LOCUS       DAZKFU020000001        65271 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZKFU020000001 DAZKFU020000000
VERSION     DAZKFU020000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.001"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..556)
                     /gene="folK"
                     /locus_tag="ACHOQQ_RS00005"
                     /old_locus_tag="pgaptmp_000001"
     CDS             complement(77..556)
                     /gene="folK"
                     /locus_tag="ACHOQQ_RS00005"
                     /old_locus_tag="pgaptmp_000001"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGU4468319.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIESQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVPQPAIW"
     gene            complement(553..1950)
                     /gene="pcnB"
                     /locus_tag="ACHOQQ_RS00010"
                     /old_locus_tag="pgaptmp_000002"
     CDS             complement(553..1950)
                     /gene="pcnB"
                     /locus_tag="ACHOQQ_RS00010"
                     /old_locus_tag="pgaptmp_000002"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGU4468320.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2010..2906)
                     /gene="gluQRS"
                     /locus_tag="ACHOQQ_RS00015"
                     /old_locus_tag="pgaptmp_000003"
     CDS             complement(2010..2906)
                     /gene="gluQRS"
                     /locus_tag="ACHOQQ_RS00015"
                     /old_locus_tag="pgaptmp_000003"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGU4468321.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPGNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2973..3428)
                     /gene="dksA"
                     /locus_tag="ACHOQQ_RS00020"
                     /old_locus_tag="pgaptmp_000004"
     CDS             complement(2973..3428)
                     /gene="dksA"
                     /locus_tag="ACHOQQ_RS00020"
                     /old_locus_tag="pgaptmp_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGU4468322.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3606..4310)
                     /gene="sfsA"
                     /locus_tag="ACHOQQ_RS00025"
                     /old_locus_tag="pgaptmp_000005"
     CDS             complement(3606..4310)
                     /gene="sfsA"
                     /locus_tag="ACHOQQ_RS00025"
                     /old_locus_tag="pgaptmp_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGU4468323.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4325..4855)
                     /gene="thpR"
                     /locus_tag="ACHOQQ_RS00030"
                     /old_locus_tag="pgaptmp_000006"
     CDS             complement(4325..4855)
                     /gene="thpR"
                     /locus_tag="ACHOQQ_RS00030"
                     /old_locus_tag="pgaptmp_000006"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGU4468324.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCIQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4929..7358
                     /gene="hrpB"
                     /locus_tag="ACHOQQ_RS00035"
                     /old_locus_tag="pgaptmp_000007"
     CDS             4929..7358
                     /gene="hrpB"
                     /locus_tag="ACHOQQ_RS00035"
                     /old_locus_tag="pgaptmp_000007"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGU4468325.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRMEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGISLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVRRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7554..10088
                     /gene="mrcB"
                     /locus_tag="ACHOQQ_RS00040"
                     /old_locus_tag="pgaptmp_000008"
     CDS             7554..10088
                     /gene="mrcB"
                     /locus_tag="ACHOQQ_RS00040"
                     /old_locus_tag="pgaptmp_000008"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGU4468326.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10308..12497
                     /gene="fhuA"
                     /locus_tag="ACHOQQ_RS00045"
                     /old_locus_tag="pgaptmp_000009"
     CDS             10308..12497
                     /gene="fhuA"
                     /locus_tag="ACHOQQ_RS00045"
                     /old_locus_tag="pgaptmp_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGU4468327.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTIEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIANDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12548..13345
                     /gene="fhuC"
                     /locus_tag="ACHOQQ_RS00050"
                     /old_locus_tag="pgaptmp_000010"
     CDS             12548..13345
                     /gene="fhuC"
                     /locus_tag="ACHOQQ_RS00050"
                     /old_locus_tag="pgaptmp_000010"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGU4468328.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLEAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVESLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13345..14235
                     /gene="fhuD"
                     /locus_tag="ACHOQQ_RS00055"
                     /old_locus_tag="pgaptmp_000011"
     CDS             13345..14235
                     /gene="fhuD"
                     /locus_tag="ACHOQQ_RS00055"
                     /old_locus_tag="pgaptmp_000011"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGU4468329.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14232..16214
                     /gene="fhuB"
                     /locus_tag="ACHOQQ_RS00060"
                     /old_locus_tag="pgaptmp_000012"
     CDS             14232..16214
                     /gene="fhuB"
                     /locus_tag="ACHOQQ_RS00060"
                     /old_locus_tag="pgaptmp_000012"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGU4468330.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFLQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16249..17529)
                     /gene="hemL"
                     /locus_tag="ACHOQQ_RS00065"
                     /old_locus_tag="pgaptmp_000013"
     CDS             complement(16249..17529)
                     /gene="hemL"
                     /locus_tag="ACHOQQ_RS00065"
                     /old_locus_tag="pgaptmp_000013"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGU4468331.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAGSVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17695..17742
                     /gene="yadX"
                     /locus_tag="ACHOQQ_RS00070"
                     /old_locus_tag="pgaptmp_000014"
     CDS             17695..17742
                     /gene="yadX"
                     /locus_tag="ACHOQQ_RS00070"
                     /old_locus_tag="pgaptmp_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGU4468332.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17754..19175
                     /gene="clcA"
                     /locus_tag="ACHOQQ_RS00075"
                     /old_locus_tag="pgaptmp_000015"
     CDS             17754..19175
                     /gene="clcA"
                     /locus_tag="ACHOQQ_RS00075"
                     /old_locus_tag="pgaptmp_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGU4468333.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19199..19264
                     /gene="yadW"
                     /locus_tag="ACHOQQ_RS00080"
                     /old_locus_tag="pgaptmp_000016"
     CDS             19199..19264
                     /gene="yadW"
                     /locus_tag="ACHOQQ_RS00080"
                     /old_locus_tag="pgaptmp_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGU4468334.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19257..19601
                     /gene="erpA"
                     /locus_tag="ACHOQQ_RS00085"
                     /old_locus_tag="pgaptmp_000017"
     CDS             19257..19601
                     /gene="erpA"
                     /locus_tag="ACHOQQ_RS00085"
                     /old_locus_tag="pgaptmp_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGU4468335.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19648..20271)
                     /gene="yadS"
                     /locus_tag="ACHOQQ_RS00090"
                     /old_locus_tag="pgaptmp_000018"
     CDS             complement(19648..20271)
                     /gene="yadS"
                     /locus_tag="ACHOQQ_RS00090"
                     /old_locus_tag="pgaptmp_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGU4468336.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20309..21109)
                     /gene="btuF"
                     /locus_tag="ACHOQQ_RS00095"
                     /old_locus_tag="pgaptmp_000019"
     CDS             complement(20309..21109)
                     /gene="btuF"
                     /locus_tag="ACHOQQ_RS00095"
                     /old_locus_tag="pgaptmp_000019"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGU4468337.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIEQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21102..21800)
                     /gene="mtnN"
                     /locus_tag="ACHOQQ_RS00100"
                     /old_locus_tag="pgaptmp_000020"
     CDS             complement(21102..21800)
                     /gene="mtnN"
                     /locus_tag="ACHOQQ_RS00100"
                     /old_locus_tag="pgaptmp_000020"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGU4468338.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21884..23401
                     /gene="dgt"
                     /locus_tag="ACHOQQ_RS00105"
                     /old_locus_tag="pgaptmp_000021"
     CDS             21884..23401
                     /gene="dgt"
                     /locus_tag="ACHOQQ_RS00105"
                     /old_locus_tag="pgaptmp_000021"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGU4468339.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23531..24955
                     /gene="degP"
                     /locus_tag="ACHOQQ_RS00110"
                     /old_locus_tag="pgaptmp_000022"
     CDS             23531..24955
                     /gene="degP"
                     /locus_tag="ACHOQQ_RS00110"
                     /old_locus_tag="pgaptmp_000022"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGU4468340.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25110..26267
                     /gene="cdaR"
                     /locus_tag="ACHOQQ_RS00115"
                     /old_locus_tag="pgaptmp_000023"
     CDS             25110..26267
                     /gene="cdaR"
                     /locus_tag="ACHOQQ_RS00115"
                     /old_locus_tag="pgaptmp_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HGU4468341.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26356..26742)
                     /gene="yaeH"
                     /locus_tag="ACHOQQ_RS00120"
                     /old_locus_tag="pgaptmp_000024"
     CDS             complement(26356..26742)
                     /gene="yaeH"
                     /locus_tag="ACHOQQ_RS00120"
                     /old_locus_tag="pgaptmp_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGU4468342.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27056..27880)
                     /gene="dapD"
                     /locus_tag="ACHOQQ_RS00125"
                     /old_locus_tag="pgaptmp_000025"
     CDS             complement(27056..27880)
                     /gene="dapD"
                     /locus_tag="ACHOQQ_RS00125"
                     /old_locus_tag="pgaptmp_000025"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGU4468343.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVYIGQSTKIYDRE
                     TGEVHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27911..30583)
                     /gene="glnD"
                     /locus_tag="ACHOQQ_RS00130"
                     /old_locus_tag="pgaptmp_000026"
     CDS             complement(27911..30583)
                     /gene="glnD"
                     /locus_tag="ACHOQQ_RS00130"
                     /old_locus_tag="pgaptmp_000026"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGU4468344.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30645..31439)
                     /gene="map"
                     /locus_tag="ACHOQQ_RS00135"
                     /old_locus_tag="pgaptmp_000027"
     CDS             complement(30645..31439)
                     /gene="map"
                     /locus_tag="ACHOQQ_RS00135"
                     /old_locus_tag="pgaptmp_000027"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGU4468345.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31807..32532
                     /gene="rpsB"
                     /locus_tag="ACHOQQ_RS00140"
                     /old_locus_tag="pgaptmp_000028"
     CDS             31807..32532
                     /gene="rpsB"
                     /locus_tag="ACHOQQ_RS00140"
                     /old_locus_tag="pgaptmp_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGU4468346.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32667..33518
                     /gene="tsf"
                     /locus_tag="ACHOQQ_RS00145"
                     /old_locus_tag="pgaptmp_000029"
     CDS             32667..33518
                     /gene="tsf"
                     /locus_tag="ACHOQQ_RS00145"
                     /old_locus_tag="pgaptmp_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGU4468347.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDSGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33665..34390
                     /gene="pyrH"
                     /locus_tag="ACHOQQ_RS00150"
                     /old_locus_tag="pgaptmp_000030"
     CDS             33665..34390
                     /gene="pyrH"
                     /locus_tag="ACHOQQ_RS00150"
                     /old_locus_tag="pgaptmp_000030"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGU4468348.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34540..35097
                     /gene="frr"
                     /locus_tag="ACHOQQ_RS00155"
                     /old_locus_tag="pgaptmp_000031"
     CDS             34540..35097
                     /gene="frr"
                     /locus_tag="ACHOQQ_RS00155"
                     /old_locus_tag="pgaptmp_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGU4468349.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35189..36385
                     /gene="ispC"
                     /locus_tag="ACHOQQ_RS00160"
                     /old_locus_tag="pgaptmp_000032"
     CDS             35189..36385
                     /gene="ispC"
                     /locus_tag="ACHOQQ_RS00160"
                     /old_locus_tag="pgaptmp_000032"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGU4468350.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36574..37332
                     /gene="ispU"
                     /locus_tag="ACHOQQ_RS00165"
                     /old_locus_tag="pgaptmp_000033"
                     /gene_synonym="uppS"
     CDS             36574..37332
                     /gene="ispU"
                     /locus_tag="ACHOQQ_RS00165"
                     /old_locus_tag="pgaptmp_000033"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGU4468351.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37345..38202
                     /gene="cdsA"
                     /locus_tag="ACHOQQ_RS00170"
                     /old_locus_tag="pgaptmp_000034"
     CDS             37345..38202
                     /gene="cdsA"
                     /locus_tag="ACHOQQ_RS00170"
                     /old_locus_tag="pgaptmp_000034"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4468352.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38214..39566
                     /gene="rseP"
                     /locus_tag="ACHOQQ_RS00175"
                     /old_locus_tag="pgaptmp_000035"
     CDS             38214..39566
                     /gene="rseP"
                     /locus_tag="ACHOQQ_RS00175"
                     /old_locus_tag="pgaptmp_000035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGU4468353.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39596..42028
                     /gene="bamA"
                     /locus_tag="ACHOQQ_RS00180"
                     /old_locus_tag="pgaptmp_000036"
     CDS             39596..42028
                     /gene="bamA"
                     /locus_tag="ACHOQQ_RS00180"
                     /old_locus_tag="pgaptmp_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGU4468354.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVQVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42150..42635
                     /gene="skp"
                     /locus_tag="ACHOQQ_RS00185"
                     /old_locus_tag="pgaptmp_000037"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42150..42635
                     /gene="skp"
                     /locus_tag="ACHOQQ_RS00185"
                     /old_locus_tag="pgaptmp_000037"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGU4468355.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42639..43664
                     /gene="lpxD"
                     /locus_tag="ACHOQQ_RS00190"
                     /old_locus_tag="pgaptmp_000038"
     CDS             42639..43664
                     /gene="lpxD"
                     /locus_tag="ACHOQQ_RS00190"
                     /old_locus_tag="pgaptmp_000038"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU4468356.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43769..44224
                     /gene="fabZ"
                     /locus_tag="ACHOQQ_RS00195"
                     /old_locus_tag="pgaptmp_000039"
     CDS             43769..44224
                     /gene="fabZ"
                     /locus_tag="ACHOQQ_RS00195"
                     /old_locus_tag="pgaptmp_000039"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGU4468357.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44228..45016
                     /gene="lpxA"
                     /locus_tag="ACHOQQ_RS00200"
                     /old_locus_tag="pgaptmp_000040"
     CDS             44228..45016
                     /gene="lpxA"
                     /locus_tag="ACHOQQ_RS00200"
                     /old_locus_tag="pgaptmp_000040"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGU4468358.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45016..46164
                     /gene="lpxB"
                     /locus_tag="ACHOQQ_RS00205"
                     /old_locus_tag="pgaptmp_000041"
     CDS             45016..46164
                     /gene="lpxB"
                     /locus_tag="ACHOQQ_RS00205"
                     /old_locus_tag="pgaptmp_000041"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGU4468359.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTEYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46161..46757
                     /gene="rnhB"
                     /locus_tag="ACHOQQ_RS00210"
                     /old_locus_tag="pgaptmp_000042"
     CDS             46161..46757
                     /gene="rnhB"
                     /locus_tag="ACHOQQ_RS00210"
                     /old_locus_tag="pgaptmp_000042"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGU4468360.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46794..50276
                     /gene="dnaE"
                     /locus_tag="ACHOQQ_RS00215"
                     /old_locus_tag="pgaptmp_000043"
     CDS             46794..50276
                     /gene="dnaE"
                     /locus_tag="ACHOQQ_RS00215"
                     /old_locus_tag="pgaptmp_000043"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGU4468361.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50289..51248
                     /gene="accA"
                     /locus_tag="ACHOQQ_RS00220"
                     /old_locus_tag="pgaptmp_000044"
     CDS             50289..51248
                     /gene="accA"
                     /locus_tag="ACHOQQ_RS00220"
                     /old_locus_tag="pgaptmp_000044"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGU4468362.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51347..53488
                     /gene="ldcC"
                     /locus_tag="ACHOQQ_RS00225"
                     /old_locus_tag="pgaptmp_000045"
     CDS             51347..53488
                     /gene="ldcC"
                     /locus_tag="ACHOQQ_RS00225"
                     /old_locus_tag="pgaptmp_000045"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGU4468363.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53545..53934
                     /gene="yaeR"
                     /locus_tag="ACHOQQ_RS00230"
                     /old_locus_tag="pgaptmp_000046"
     CDS             53545..53934
                     /gene="yaeR"
                     /locus_tag="ACHOQQ_RS00230"
                     /old_locus_tag="pgaptmp_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4468364.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53999..55297
                     /gene="tilS"
                     /locus_tag="ACHOQQ_RS00235"
                     /old_locus_tag="pgaptmp_000047"
     CDS             53999..55297
                     /gene="tilS"
                     /locus_tag="ACHOQQ_RS00235"
                     /old_locus_tag="pgaptmp_000047"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGU4468365.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(55346..55606)
                     /gene="rof"
                     /locus_tag="ACHOQQ_RS00240"
                     /old_locus_tag="pgaptmp_000048"
     CDS             complement(55346..55606)
                     /gene="rof"
                     /locus_tag="ACHOQQ_RS00240"
                     /old_locus_tag="pgaptmp_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGU4468366.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55593..55793)
                     /gene="yaeP"
                     /locus_tag="ACHOQQ_RS00245"
                     /old_locus_tag="pgaptmp_000049"
     CDS             complement(55593..55793)
                     /gene="yaeP"
                     /locus_tag="ACHOQQ_RS00245"
                     /old_locus_tag="pgaptmp_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGU4468367.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55959..56504
                     /gene="yaeQ"
                     /locus_tag="ACHOQQ_RS00250"
                     /old_locus_tag="pgaptmp_000050"
     CDS             55959..56504
                     /gene="yaeQ"
                     /locus_tag="ACHOQQ_RS00250"
                     /old_locus_tag="pgaptmp_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGU4468368.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56501..56923
                     /gene="arfB"
                     /locus_tag="ACHOQQ_RS00255"
                     /old_locus_tag="pgaptmp_000051"
     CDS             56501..56923
                     /gene="arfB"
                     /locus_tag="ACHOQQ_RS00255"
                     /old_locus_tag="pgaptmp_000051"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGU4468369.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKELTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56937..57647
                     /gene="nlpE"
                     /locus_tag="ACHOQQ_RS00260"
                     /old_locus_tag="pgaptmp_000052"
                     /gene_synonym="cutF"
     CDS             56937..57647
                     /gene="nlpE"
                     /locus_tag="ACHOQQ_RS00260"
                     /old_locus_tag="pgaptmp_000052"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGU4468370.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQVAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57847..58671)
                     /gene="yaeF"
                     /locus_tag="ACHOQQ_RS00265"
                     /old_locus_tag="pgaptmp_000053"
     CDS             complement(57847..58671)
                     /gene="yaeF"
                     /locus_tag="ACHOQQ_RS00265"
                     /old_locus_tag="pgaptmp_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HGU4468371.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDVEAKTWAV
                     KFQHQSSFTEQAIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQICSLNPFSADFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58724..60442)
                     /gene="proS"
                     /locus_tag="ACHOQQ_RS00270"
                     /old_locus_tag="pgaptmp_000054"
     CDS             complement(58724..60442)
                     /gene="proS"
                     /locus_tag="ACHOQQ_RS00270"
                     /old_locus_tag="pgaptmp_000054"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGU4468372.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVNAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60553..61260)
                     /gene="tsaA"
                     /locus_tag="ACHOQQ_RS00275"
                     /old_locus_tag="pgaptmp_000055"
     CDS             complement(60553..61260)
                     /gene="tsaA"
                     /locus_tag="ACHOQQ_RS00275"
                     /old_locus_tag="pgaptmp_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGU4468373.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MTVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61257..61661)
                     /gene="rcsF"
                     /locus_tag="ACHOQQ_RS00280"
                     /old_locus_tag="pgaptmp_000056"
     CDS             complement(61257..61661)
                     /gene="rcsF"
                     /locus_tag="ACHOQQ_RS00280"
                     /old_locus_tag="pgaptmp_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGU4468374.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61779..62594)
                     /gene="metQ"
                     /locus_tag="ACHOQQ_RS00285"
                     /old_locus_tag="pgaptmp_000057"
     CDS             complement(61779..62594)
                     /gene="metQ"
                     /locus_tag="ACHOQQ_RS00285"
                     /old_locus_tag="pgaptmp_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGU4468375.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62634..63287)
                     /gene="metI"
                     /locus_tag="ACHOQQ_RS00290"
                     /old_locus_tag="pgaptmp_000058"
     CDS             complement(62634..63287)
                     /gene="metI"
                     /locus_tag="ACHOQQ_RS00290"
                     /old_locus_tag="pgaptmp_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGU4468376.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63280..64311)
                     /gene="metN"
                     /locus_tag="ACHOQQ_RS00295"
                     /old_locus_tag="pgaptmp_000059"
     CDS             complement(63280..64311)
                     /gene="metN"
                     /locus_tag="ACHOQQ_RS00295"
                     /old_locus_tag="pgaptmp_000059"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGU4468377.1"
                     /translation="MIKLSNITKVFHQGTRIIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64499..65074
                     /gene="gmhB"
                     /locus_tag="ACHOQQ_RS00300"
                     /old_locus_tag="pgaptmp_000060"
     CDS             64499..65074
                     /gene="gmhB"
                     /locus_tag="ACHOQQ_RS00300"
                     /old_locus_tag="pgaptmp_000060"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGU4468378.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFINGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15446 a        16175 c        17961 g        15689 t
ORIGIN      
        1 aataattttt cttggtggat gaaatagccg tatcattttg tattcctctc gtcaagaaaa
       61 taaaataata cagctattac cagatggcag gttgtggtac tgctagtgaa gtgagtattt
      121 cacgtaatgt ttccccatca ggaaacacca actccggcgc gatttcaaac agcggccaca
      181 gcataaatcc acgatttttc atatcgtagt gcggaacggt caggcgttcg gtatttatta
      241 cttcattacc aaacagcatg atgtcgagat ccagcgtgcg cggtccccag cgttcagctt
      301 tacggacgcg accttgctgc gattcaatac gctgtgtgtg attgagtagc tcttcaggtg
      361 caagagaggt ttccagcgct acggctgcat ttaagtaatc gggttgatct tgcggcccca
      421 gcggtggagt gcggtaaaac gaagaaacgg caagaatgtg gctttcaggg atatcgccta
      481 atgctttcag ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa
      541 tatacgccac tgtcatgcgg taccctcacg acgtggtgcg cgtttgcgtg gacgacgagt
      601 acgacgacgc ggtgacggtt cttcatccag ctcgttgagc atcccttttt ggtctggtgg
      661 cgcggaaacc tggaactcac cccaccattt caccagacgc tgcagttcag cgttacgttc
      721 aacttcagct cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag
      781 tttccatgca cgtttaccct gacgtcggga catacgcaac tgcaactgcc agatatcgcg
      841 ggttaacgtc gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc
      901 gttcatcgcc agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg
      961 tgccgtctcc agcagtgggt accagaacat ggcggcaaac aaaaacgccg ggttaacgcg
     1021 catatcgtta tgaatacgcg tatcggtgtt cttcagtacc tgctcaatga tccgctccat
     1081 tgggctgtcg ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag
     1141 atgatattca cacagcagct tataggtttc gtaaccgtag cccgcttgta gcagtttaag
     1201 cgattcttca aacaggcgtg ccggtgggat atcgttcagc agggtagcga ggcgagggat
     1261 cggttctgcg gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtactgcgcg
     1321 cagcatacgt accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac
     1381 accgtccttc agatccttca taccgccaac gtaatcacgg acggtaaaat ccgctacgct
     1441 gtaatacagg ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa
     1501 aatgttgtcg cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac
     1561 gttaccttcg tggtgtccac ggaaggtcgc aacttcgata atctccgggc caaacatcac
     1621 atgagccaga cggaaacggc gacccaccag gcggcagtta cggaacagtt tgcgcacctg
     1681 ctcaggcgtg gcgttagtgg ttacgtcaaa gtctttcggc tttttgccaa gtaacaggtc
     1741 gcgcacgccg ccgccaacca gccaggcttc gtatcccgct ttattgagcc tgtacattac
     1801 cttcagggca ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac
     1861 cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt
     1921 gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc
     1981 aggacaaaaa aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt
     2041 acaattgccg actcaggcac tgcggtaagc cgccagtttt tgacggctga ctgaaggatt
     2101 tgttcgacgc tgaaatcctg ccagtgtgct tctacctgct gacccagaaa ttgaagtgcc
     2161 gcgattagta ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa
     2221 agtttagcgc cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg cactttccag
     2281 ccaaaaagct ggtacagcga gatttgccgc actgtcggtt cgatcaaatc cgccccgcgg
     2341 acaatttcgc tcacgccctg gaagtgatca tccaccacca ccgccaggtt ataggcaaac
     2401 aaaccatcac ggcgatgaat aataaaatct tcccgtgcca gtttttcgtc ggcgtgaata
     2461 atgcctcgca gcaggtcagt aaactgggtg accggatgct gctggcggat acgcactgcg
     2521 gcgttgcctg gtccatgatg caattcccgg caatgaccgt cgtaaatacc gccaatgctt
     2581 tgaatacgtg cacgcgtgca ggtgcagtaa taacttagtc cttgctcatg taaccaggcg
     2641 agtgcttcgc gataggcgtg gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc
     2701 aggccgtaat gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc
     2761 gggtcgatat cttctatgcg taccagccag cgaccttgcc gggcgcgagc ctgcaaatag
     2821 ctgccaagcg cagcaatcag agaaccaaaa tgaagctcgc cggaaggaga gggggcgaag
     2881 cggccaatat agtgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc
     2941 cgcctgtggt aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt
     3001 tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga
     3061 ataccaattt caacaccgca ggattcacag tagccgaaat cttcgtcttc cacttttttc
     3121 agcgtcttct cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg
     3181 ctgaactctt cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc
     3241 tgcatatgtg taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga
     3301 atacgacgga agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga
     3361 tatggttcca ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct
     3421 tcttgcatgt tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga
     3481 ggccgctata aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca
     3541 actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac cagttactta
     3601 ctttactaca atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc
     3661 gctttgtaag ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat
     3721 ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa
     3781 aagataaccg cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga
     3841 cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat
     3901 ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg
     3961 cgttctgcgc cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt
     4021 gattcattaa ggatagcctc tttcgtcaac ctgttagccc aaagcgtgtt gacgcaaata
     4081 aatgcgccgc tctggctttg agttaattcc caggtgtgtg ggtatttccg tttagtgttg
     4141 tctgaagtcg aataccagac ggtatcgcca ggcgttgcac aaccggtcat cgcacccgta
     4201 ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt
     4261 ttgtaacgct gaattagcgt cgcgcgctgt agagggggag aaaattccat tgcgacaatc
     4321 cttgttattg cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa
     4381 acgaggaggc gtaaagggtg aactccgtca ccgcatacga ccagttaaaa cctggcggcg
     4441 ggattgtcac cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat
     4501 tgctttgaat acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta
     4561 agccgcgtgg aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg
     4621 cgtcatcaag cgtgagtgtg aaaccaggtt gacgaatccg tccggctaaa agagaaagcg
     4681 ccttctcttt ctcggcgctc acttcgccta aaaatgccag agtcagatgc aaattatcgg
     4741 cggcgaccgg acgtcccgcc tcaagtggga agtgtgtggc gcgccagtgg ataatctgtt
     4801 cgcggatttc tgcaggtaag tcgatagcaa agaacagacg ttgcgggtca gacatgtgag
     4861 gcactcggtt atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg
     4921 agcgttttgt gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt accgctctcg
     4981 attgtgcgcc gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc
     5041 cgctgcaact gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc
     5101 gtctggcggc gcgtaacgtc gcgcaacggc tggcggaact gcttaacgaa aagccaggcg
     5161 ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc cgcatggaag
     5221 tagttaccga aggcgtgctg acgcggatga tccagcgtga cccggaactg agcggtgttg
     5281 gactggtgat ccttgatgaa tttcatgagc gcagcttgca ggcggatttg gcgttggcgc
     5341 tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta
     5401 cgctggataa cgaccgcttg cagcaaatgc tgccagaagc gcccgtcgtc atctcagaag
     5461 ggcgctcgtt tcctgttgaa cgccgttatt taccgctgcc cacgcatcag cgttttgacg
     5521 aagccgtcgc ggttgccacc gccgaaatgc tgcgtcagga aagtggatca ttactgttat
     5581 ttttacctgg cgtcggagaa attcagcgtg tgcaggaaca actggcttcg cgcatcggca
     5641 gtgatgtgtt gctctgcccg ctgtatggcg cgttgtcgct gaacgatcag cgaaaagcga
     5701 tcctcccggc accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca
     5761 gtttaaccat tgaaggtatt agtctggtgg tggattgtgc ccaggagcgt gtggcgcgtt
     5821 ttgatccgcg cacggggctt acgcgactga ttactcaacg cgttagccag gcatccatga
     5881 cgcagcgtgc cgggcgcgcc gggcgtctgg agccgggtat ctgcctgcat ttaatcgcca
     5941 aggaacaagc agaacgcgcc gcggcgcaaa gtgaaccgga gatcttacaa agcgatcttt
     6001 ccggtttgct gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc
     6061 tggatcaacc gccaacggtg aatctactgg ccgcgaaacg tctgttacaa atgctggggg
     6121 cgctggacgg tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc
     6181 cgcgtttagc ggcaatgctg gtgagcgcga agagcgacga cgaagctgct accgcggcaa
     6241 aaattgccgc cattctcgaa gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt
     6301 tttcgcgcaa tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgcttaaacg
     6361 tacgcggcgg tgaggcagac agttcgctta tcgcgccgct acttgccggg gcgtttgccg
     6421 atcgcattgc tcgccgccgt gggcaagatg gacgctatca actggcaaac ggcatgggag
     6481 cgatgctcga tgccgacgac gcgctaagcc gccacgaatg gttgatcgca ccgttattat
     6541 tgcagggcag cgcctcgccg gatgcgcgga ttttactggc gctaccggtc gatattgatg
     6601 aattagtgcg acgctgtccg cagctggtgc agcaatctga caccgtggaa tgggatgacg
     6661 cgcaaggtac gctgaaagcc tggcgtcggc tgcaaatcgg tcagttgacg gtgaaagtgc
     6721 agccgttggc gaaaccgtca gaagacgagt tgcatcaggc gatgcttaac ggcatccgtg
     6781 ataaaggttt aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt
     6841 tatgcgccgg aaagtggttg ccagaatatg actggccagc ggttgatgat gaaagtttgt
     6901 tggcgacgct ggaaacgtgg ctgctgccac atatggccgg tgtacattca ctacgcggcc
     6961 tgaaatcact cgacatttat caggcactac gcggattact tgattgggga atgcagcaac
     7021 gtctggatag tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc
     7081 gttatcatga agataacccg cccgcgctgg cggtgagaat gcaggagatg tttggtgagg
     7141 ccaccaatcc gacgatcgcc caggggcgcg tgccgctggt gctggagttg ctttcccctg
     7201 cccaaaggcc gctgcaaatc acgcgtgatt tgggggcctt ctggaaaggg gcgtatcgtg
     7261 aggtgcaaaa ggagatgaaa gggcgttatc ccaaacacgt ctggccggac gacccggcaa
     7321 acacggcacc gacgcgacgg acgaaaaagt attcgtaagg ttggttttct ccctctccct
     7381 gtgggagagg gccggggtga gggcatcagc gcgcacgttc accctcaccc taaccctctc
     7441 ccttaaggga gaggggaccg atcgagcaca aattttgaga gatatcttct tctgtcttgt
     7501 aacagaagaa cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa agcatggccg
     7561 ggaatgaccg cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg
     7621 taagccgtcg tcgttacgaa gatgacgatg attacgacga ttatgatgac tatgaggatg
     7681 aagaaccgat gccgcgcaaa ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac
     7741 gcggctggct atggctgctg ttaaaactgg ctatcgtttt tgccgtgctg atcgccattt
     7801 acggcgttta tctcgatcaa aaaattcgta gccgtattga tggcaaggtc tggcaactgc
     7861 ctgcggcagt ttatggtcga atggtcaacc ttgagccaga catgaccatc agcaagaacg
     7921 aaatggtgaa gctgctggag gcaacccagt atcgtcaggt gtcaaaaatg acccgtcctg
     7981 gcgaatttac cgtgcaggcc aacagcattg agatgattcg ccgtccgttt gatttcccgg
     8041 acagtaaaga aggacaggtg cgcgcgcgtc tgacctttga tggcgatcat ttggcgacga
     8101 tcgtcaatat ggagaacaac cgtcagttcg gtttcttccg tcttgatccg cgtctgatca
     8161 ccatgatctc ctcaccaaac ggtgagcagc gtttgtttgt gccgcgcagt ggtttcccgg
     8221 atttgctggt ggatactttg ctggcgacag aagaccgcca tttctacgag catgatggaa
     8281 tcagtctcta ctccatcgga cgtgcggtgc tggcaaacct gacagccgga cgcacggtgc
     8341 agggggcgag tacgctgacg caacagctgg tgaaaaacct gttcctctcc agcgagcgct
     8401 cttactggcg taaagcgaac gaagcttaca tggcgctgat catggacgcg cgttacagca
     8461 aagaccgtat tcttgagctg tatatgaacg aggtgtatct cggtcagagt ggcgacaacg
     8521 aaatccgcgg cttcccgctg gcgagcttgt attactttgg tcgcccggta gaagaattga
     8581 gcctcgatca acaggcgctg ttagtcggta tggtgaaagg agcgtccatc tacaacccgt
     8641 ggcgtaaccc gaaactggcg ctggagcgac gtaatctggt actgcgtctg ctgcaacagc
     8701 aacagattat tgatcaagaa ctctatgaca tgttgagtgc gcgtccgctg ggtgttcagc
     8761 cgcgcggtgg ggtgatctct cctcagccag cctttatgca actggtgcgt caggagcttc
     8821 aggctaaatt aggcgataag gtaaaagatc tctccggcgt gaagatcttc actacctttg
     8881 actcggtggc ccaggacgcg gcagaaaaag ccgccgtgga aggcattccg gcacttaaga
     8941 aacagcgtaa gctgagcgat ctggaaaccg cgattgtggt cgtcgaccgc tttagtggtg
     9001 aagttcgtgc gatggtcgga ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc
     9061 aggcgcggcg ttcgattggt tcccttgcaa aaccagcgac ttatctgacg gctttaagcc
     9121 agccgaaaat ctatcgtctg aatacgtgga ttgcggatgc gccaatcgcg ctacgtcagc
     9181 cgaatggcca ggtctggtca ccgcagaacg atgaccgtcg ttatagcgaa agcggcagag
     9241 tgatgctggt ggatgcgttg acccgttcga tgaacgtgcc gacggtaaat ctggggatgg
     9301 cgctggggct gcctgcggtt acggatacct ggattaaact gggcgtgccg aaagatcagc
     9361 tgcatccggt tccggcgatg ctgctggggg cgttgaactt aacgccaatc gaagtggcgc
     9421 aggcattcca gaccatcgcc agcggcggta accgtgcacc gctttctgcg ctgcgttcgg
     9481 taattgcgga agatggcaaa gtgctgtatc agagcttccc gcaggcggaa cgcgctgttc
     9541 cggcgcaggc ggcgtatctg acactatgga ccatgcagca ggtggtacaa cgcggtacgg
     9601 gtcgtcagct tggggcgaaa tacccgaacc tgcatctggc agggaaaaca gggactacca
     9661 acaataacgt agatacatgg tttgcgggca ttgacggcag cacggtgacc atcacctggg
     9721 tcggccgtga taacaaccag ccgaccaaac tgtatggtgc cagcggggca atgtcgattt
     9781 atcagcgtta tctggctaac cagacgccaa cgccgctgaa tcttgttccg ccagaagata
     9841 ttgcagatat gggcgtggac tacgacggca actttgtttg cagcggtggc atgcgtgtct
     9901 tgccggtctg gaccagcgat ccgcaatcgc tgtgccagca gagtgagatg cagcagcagc
     9961 cgtcaggcaa tccgtttgat cagtcttctc agccgcagca acagccgcaa cagcaacctg
    10021 ctcagcaaga gcagaaagac agcgacggtg tagccggttg gattaaagat atgtttggta
    10081 gtaattaacg tctaaacgtg acataccgga tggcgagttg ccatccggta aaataacatc
    10141 ccacctaaag tattaaccct ttcttttcat ctggttgttt attaaccctt caggaacgct
    10201 cagattgcgt accgcttgcg aacccgccag cgtttcgaat attatcttat ctttataata
    10261 atcattctcg tttacgttat cattcacttt acatcagaga tataccaatg gcgcgttcca
    10321 aaactgctca gccaaaacac tcactgcgta aaatcgcagt tgtagtagcc acagcggtta
    10381 gcggcatgtc tgtttatgca caggcagcgg ttgaaccgaa agaagacact atcaccgtta
    10441 ctgctgcacc tgcgccgcaa gaaagcgcat gggggcctgc tgcaactatt gcggcgaagc
    10501 actctgctac tgcgactaaa acggatacac caattgaaaa aacgccgcag tctatttcgg
    10561 ttgtgacaaa tgaagagatg cagatgcatc aatttcagtc tgtaaaagaa gcattaggtt
    10621 atacacctgg tgttacggtt agcagtcgtg gtgcttctaa tacatatgat tttgttatca
    10681 ttcgtggttt ctcatctgtt ggtctgaatc aaaataacta ccttgatgga ctaaaacttc
    10741 agggcaactt ttataacgat gctgtgattg atccttacat gctcgagagg gttgaactga
    10801 tgcgtgggcc gacgtctgtt ctctatggaa aaagtaatcc tggtgggatt atttctatgg
    10861 tgagtaagcg tccgaccatt gaacccctga aagaaattca gtttaaaatg gggacggata
    10921 atctgtttca gaccggattc gatttcagtg atgcgctgga tgataacggt gagttctcct
    10981 accgtttgac ggggctcgct cgttcaacaa acgaacagca gaaaaattct gaatctcagc
    11041 gttataccat tgctccatca ttctcatggc gtccggacga caaaaccaat tttactttcc
    11101 tgtcctattt ccagaatgaa ccagaaacgg gttattacgg ttggttgccg aaagagggga
    11161 ccgttgagcc attgcctaat ggtaaacgtc taccgactga cttcaatgaa ggtgcatcga
    11221 ataatacata ctcccgtaac cagaaaatgg tgggatatag ttttgaacat ggttttaatg
    11281 acaccttcac cgtgcgtcag aatctgcgtt tcagtgaaat gaaaacctca cagaaaagtg
    11341 tttatggcac agggattgcc aacgatggtc atactctaaa ccgcgggaca gtggtggata
    11401 atgagcgtct gcaaaacttt agcgttgata cccaacttga aagtaaattt gctacaggtg
    11461 aagtcgagca tactttgctg acaggggtag acttcatgcg tatgcgcaat gatatcaatg
    11521 ccagctttgg atctgcacca tccatcgatc tttataacaa atatcatcct gaatactttg
    11581 catttggtaa cgcagagcca taccaaatga atgaaagcaa acaaacaggt atttatgttc
    11641 aggatcaggc ggaatggaat aaatgggtat tcactctggg gggacgctac gattggtcta
    11701 agcaagcgac cactgttcgt gaaaactctt atacgccgac tgaaggttat attgagcgta
    11761 atgatcatca gttcacctgg cgcggtggtg taaactactt attcgataat ggtatttcac
    11821 cttactttag ctatagccag tcctttgaac cgagtgcttt cgatctgtgg agcaacccgc
    11881 gcgtttccta taagccatcg aaaggtgaac agtatgaagc tggcgtaaaa tatgttccga
    11941 atgatatgcc ggtcgttgtt acgggcgcag tctatcaatt gacgaaaaca aataacctga
    12001 cagcagaccc aacaaacccg ttagcgcaag tcccagcagg tgagattcgc gctcgtggtg
    12061 tggaacttga agcaaaagcg gcgttaaatg ccaatattaa cttgacggct tcttatacct
    12121 acacggatgc ggaatacacc aaagacacca atctcaaagg taaaactcct gaacaagtac
    12181 cggagcatat ggcatctctc tggggggatt ataccttcaa tgaagggccg ctttctggtt
    12241 taacattggg aacaggtggt cgttttattg gttccagcta tggtgatccg gcaaacactt
    12301 ttaaagtggg tagcgcagct gtaatggatg ctgttgtaaa atatgatctg gcacgctttg
    12361 gtatggcggg atccagcctt gctgtgaacg tcaacaattt gctcgatcgt gagtatgttg
    12421 ccagttgctt ccagacctat ggctgcttct ggggcgcaga acgtcaggtc gttgcaaccg
    12481 caaccttccg tttctaattt tctctttggg gcacgggttt ccgtgcccgc ttcacaagtt
    12541 ggctgttatg caggaataca cgaatcattc cgataccact tttgcactgc gtaatatctc
    12601 ctttcgtgtg ccagggcgca cgcttttgca tccgctgtcg ttaacctttc ctgccgggaa
    12661 agtgaccggt ttgattggtc acaacggttc tggtaaatcc acgctgttaa aaatgttggg
    12721 ccgtcatcag ccgccgtcag aaggtgaaat tcttcttgaa gcccagccgc tggaaagctg
    12781 gagtagcaaa gcgtttgccc gcaaagtggc ttatttgccg cagcaacttc ctccggcaga
    12841 agggatgact gtgcgtgaac tggtggcgat tgggcgttac ccgtggcatg gcgcgctggg
    12901 gcgctttggt gcggcagatc gcgaaaaggt cgaggaagct atctcgctgg ttggcttaaa
    12961 accgctggcg catcggctgg tagaaagcct ctctggcggc gaacgtcagc gggcgtggat
    13021 cgccatgctg gtggcgcagg atagccgttg tctgttgctc gacgaaccga cttcggcgtt
    13081 ggatatcgcc caccaggttg atgtgctggc gctggtgcac cgtttaagtc aggagcgtgg
    13141 cctgacggtc attgccgtgc tgcacgatat caatatggct gcgcgttact gtgattatct
    13201 ggtcgccctg cgcggcggtg aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg
    13261 cgaaaccctc gaaatgattt atggcatccc gatgggtatt ctgccgcatc cggcgggtgc
    13321 tgcacctgtg agttttgttt attgatgagc ggcttacctc ttatttcgcg ccgtcgactg
    13381 ttaacggcga tggcgctttc tccgttgtta tggcagatga ataccgccca cgcggcggct
    13441 attgacccaa atcgtattgt ggcgctggag tggctgccgg tggaattact actggcgctc
    13501 ggcatcgtgc cttacggcgt ggcggacacc atcaactatc gcctgtgggt cagcgaacca
    13561 ccactacctg aatcggtgat tgacgtcggt ttgcgtacag aacctaacct cgaactgctg
    13621 accgaaatga aaccgtcgtt tatggtctgg tcggcgggat atggtccttc gccagaaatg
    13681 ctggctcgta ttgcgccggg gcgtggattt aacttcagcg acggcaaaca gccgctggcg
    13741 atggcgcgta aatcgctgac ggaaatggcg gatttactta acctgcaaag cgcagcagaa
    13801 acgcatttag cgcaatatga agactttatc cgcagcatga aaccccgctt tgtgaagcgt
    13861 ggtgcgcgtc cgttattgct gacgacgctt atcgatccgc gccatatgct ggtcttcggt
    13921 ccaaacagct tgttccagga aattcttgat gagtacggca tcccaaatgc ctggcaaggg
    13981 gaaaccaact tctggggcag taccgccgtc agcatcgatc gtctggcagc gtataaagac
    14041 gttgatgtgc tctgttttga tcacgacaac agcaaagaca tggatgcgct aatggcaacg
    14101 ccgctgtggc aggccatgcc gtttgtccgc gccggacgct ttcagcgcgt acccgcagtc
    14161 tggttttatg gtgcaacgct ctcggcaatg cactttgtgc gcgttctgga taacgccatt
    14221 ggaggtaaag cgtgagtaaa cgtattgcgc ttttcccggc gttattgctg gcgctgctgg
    14281 tgattgtcgc tacggcgctg acctggatga actttttgca ggcgctgccg cgtagccagt
    14341 gggcgcaggc cgcctggtcg ccggatatta acgtcatcga gcagatgatt tttcactaca
    14401 gcttgttacc gcgtctggcg atttctctac tggtgggcgc gggcctgggg ctggtgggcg
    14461 tgctgtttca gcaagtgctg cgtaacccgc tggcggaacc gacgacgttg ggcgttgcta
    14521 caggcgcgca actggggatt accgtcacga cgctctgggc gatccctggt gcgatggcga
    14581 gccagtttgc tgcgctggca ggggcttgtg ttgttggctt aattgtcttt ggcgtcgcgt
    14641 gggggaaacg gctgtcgccg gtaacgctga ttctcgcggg gttggtagtg agcctttatt
    14701 gcggcgcaat caatcagtta ctggttatct tccatcatga ccaactgcaa agcatgtttc
    14761 tgtggagcac tggaacgctg acgcaaaccg actggggcgg tgttgagcgt ttatggccgc
    14821 agttgctggg cggtgtgatg ctgacgttgc tgctacttcg tccgttaaca ctgatggggc
    14881 ttgatgatgg cgtggcgcgc aatctcgggc tagccttgtc gctggcgcgt ctggcggcgc
    14941 tgtcgctggc tattgtcatc agtgcgctgc tggtgaacgc ggtggggatt atcggcttta
    15001 tcgggttgtt cgcgccgttg ctggcaaaaa tgctgggggc gcggcgtctg ctgccgcgac
    15061 tgatgctggc gtcgttgatt ggtgcgctga tcctctggct ttccgatcaa atcatcctct
    15121 ggctgactcg cgtgtggatg gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc
    15181 cgctgctact gtggttgctg ccgcgtttac gcagcattag cgcgccggat atgaaggtca
    15241 acgatcgtgt cgcggctgaa cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc
    15301 tgctgttgat ggctgtggtg gtggcgctgt cgtttggtcg tgatgcgcac ggctggacgt
    15361 gggcgagcgg ggcgttgctc gatgatttaa tgccctggcg ctggccgcga attatggcgg
    15421 cgctgtttgc gggcgtcatg ctggcggtgg cgggctgtat tattcagcgg ctgaccggca
    15481 acccgatggc aagcccggaa gtgctgggga ttagctctgg cgcggcgttt ggcgtggtgt
    15541 taatgctgtt tctggtgccg ggtaatgcct ttggctggct gttacccgcg ggcagtatcg
    15601 gtgccgcggt gacgctgttg attattatga tcgccgccgg gcgcggtgga ttttccccac
    15661 accgcatgtt actggcgggg atggcgttaa gtaccgcatt caccatgctt ttaatgatgt
    15721 tgcaggcgag tggtgacccg cgaatggcgc aagtgctgac ctggatctct ggttcgacct
    15781 acaacgccac cgatgcgcag gtctggcgca ccggaattgt gatggtgatt ttgctggcga
    15841 ttaccccgct gtgccgccgc tggctgacca ttttaccgct gggtggtgat accgcgcggg
    15901 ccgtgggggt ggcgttaacg ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga
    15961 cggcgaccgc gacgatgacc attggaccgt tgagctttgt tggtttaatg gcaccgcata
    16021 tcgcgcggat gatgggcttt cgacggacga tgccgcacat cgtgatttcg gcgctggtgg
    16081 gtggtttact gctggtgttc gctgactggt gtgggcggat ggtgctgttt ccgttccaga
    16141 tcccggcggg gctgctgtcg acctttatcg gcgcgccata ttttatctat ttgttgagaa
    16201 agcagagccg ttaataaaat gcctcgcccg gtgggcgagg caaaatcatc acaacttcgc
    16261 aaacacccga cgtgcagcat cgatggtgtt attgatatct tccatgctgt gcgccacaga
    16321 cataaagccc gcttcaaacg ctgacggtgc caggtaaaca ccttcatcca gcatcatatg
    16381 gaagaaacgc ttaaaacgct cgacgtcaca ggccatcaca tcctgatagc acgtcacgga
    16441 cccggcgtcg gtaaagaaaa taccgaacat gccgccaacg tggtttacca ccagcggaat
    16501 tccggcttct tctgccgctt ccagcagacc ttctgccaga cgtgttgtca gctcatccag
    16561 cgtttcgtga acgcccggct gcgcgacttc attcagacag gcgaaaccgg ctgccatcgc
    16621 aatcgggtta ccggaaagcg tacccgcctg atagaccgga cccgtcgggg ccagcgcatc
    16681 cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga tgattttgcc
    16741 gaggcaggtt aaatctggca ctacgccgta ataatcctgt gcgccagcta gcgctacgcg
    16801 gaaaccggtc atcacttcat cgatgatcag caacgcgcca aattcgtcac acagcgcacg
    16861 cagacctggc aggaactctg gcagcggcgg aacacagttc atattgcctg ccaccggctc
    16921 gacgataata caggcaatct cttgcgggta ttgctcaaac gcggcgcgta cagaagccag
    16981 atcgttataa gtacaggtta aggtatgttt ggcgaaatct gccggaacgc ccggcgagtt
    17041 tggctggcct aacgtgagtg cgccagaacc ggcttttacc agcaggcagt cagcgtgacc
    17101 gtggtaacaa ccttcaaatt taataatttt gtcgcgaccg gtaaaaccac gggccaggcg
    17161 gatggcgctc atggtcgcct cggtgccgga gttcaccatg cgcaccatat ccatggtcgg
    17221 gaccagttcg gtcaccagtt gcgccatttt cacttccatt tcggtcggtg caccaaagct
    17281 taaaccacgc tcggcggctt caatcacggc attgcggatc gccggatggt tatggcccag
    17341 caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa catcgtacag
    17401 ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag taaaggcgcg
    17461 aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa gattttcaga
    17521 cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt ctatgttatt
    17581 cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa tcctgatttc
    17641 ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat tttcatggaa
    17701 cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat tggatgaaaa
    17761 ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga caactgattc
    17821 gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg
    17881 gcacgcttgt cgggctggct gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc
    17941 aacgtatggg ggcgctggta catactgctg ataattatcc gcttctgtta accgtcgctt
    18001 ttctctgttc ggcggtgctg gcgatgttcg gctacttttt agtgcgcaaa tacgcgccgg
    18061 aagcaggtgg ttcggggatc ccggaaattg aaggggcgct ggaagatcaa cgtcccgttc
    18121 gctggtggcg tgtattgccg gtgaaattct ttggcgggct ggggacactc ggcggaggca
    18181 tggtgctagg gcgcgaaggg ccaaccgtgc agatcggcgg taatatcggg cggatggtac
    18241 tcgatgtttt ccgcctgaaa ggtgatgaag ctcgccatac gctgttggca accggtgcgg
    18301 cggcgggtct ggcggcggcc tttaacgcgc cgctggcggg tattttgttt attatcgaag
    18361 agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa agcggtattt attggcgtca
    18421 ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt gacgtcggta
    18481 aactttctga cgcgccgctt aatacgctgt ggctttatct gatcctcggt attatttttg
    18541 gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg ctgcaccgtg
    18601 tgcacggcgg caatattacc aaatgggtac tgatgggtgg tgcgattggc ggtctgtgtg
    18661 gattgctggg gtttgttgca ccagcaacgt cgggcggcgg ttttaacctg attcctatcg
    18721 ctaccgcggg gaatttcagc atgggaatgc tggtgtttat cttcgtcgcg cgggtcatta
    18781 ccaccttact ctgcttctct tccggcgcgc cgggcggtat ttttgccccg atgctggcgc
    18841 tgggtactgt gctgggaacc gctttcggaa tggttgccgt tgagctgttt ccgcaatatc
    18901 accttgaggc ggggacgttt gctattgccg gaatgggggc gttattggcg gcatctattc
    18961 gcgcgccgtt aacggggatc attctggttc tggagatgac cgataactac cagctcattt
    19021 tgccaatgat tattaccggt cttggcgcaa cactattagc gcaatttacc ggcgggaaac
    19081 cgctatactc ggcgattctt gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc
    19141 gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa ataccagggt attagataat
    19201 ggcgattatt attgggttag aatttgccca attgccgatg tcgtttggag caaaatatga
    19261 gtgatgacgt agcactgccg ctggagttta ccgacgcagc agccaacaaa gttaaaagcc
    19321 tgatcgctga cgaagataac ccgaatctga aattacgcgt gtatatcacc ggtggcggtt
    19381 gcagcggctt ccagtatggt ttcacctttg atgatcaggt gaacgaaggc gatatgacca
    19441 tcgaaaaaca gggcgttggc ctggtggttg atccgatgag cctgcaatat ctggtcggcg
    19501 gttccgttga ctataccgaa ggactggaag gttctcgttt catcgtgacc aacccgaacg
    19561 cgaaaagcac ctgcggttgc ggttcttcct ttagtatcta atctgttgtt catgtgccgg
    19621 ataaactatc cggcacatct tctctcttca acgcccattc tcatccagcg caaacgtcgg
    19681 tagcttaaga tgccaacgaa tagccgccag ccgaatcaat agcgtcacga ccatgcccat
    19741 catactggct gtttccagtg gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc
    19801 gccgataata caggcagttg cgtagatttc tgtacgtaaa atcatgggga tttcgcgggc
    19861 cagaacatca cgaatgatcc cgccgccaac gccagtaatg acgcccatac aaaccgcgat
    19921 taacggaccg gcttccgcat taaaggcttt attcacgcca atgccgacaa acaccgccag
    19981 accaacggcg tccagcaccg gcaacatcca ttttggtaag cgtcttggct ggcgcaccag
    20041 cacgatggtc agcatgctgg ttaccattgc aacgaccaga tcggtgggat ctttcaccca
    20101 aaataccggg ccgtgatcca gcgccatgtc gcgaattgtc ccgccgccta cagcggtaac
    20161 cacgcccagt accagaacac caaaagggtc catacgcaat tttccggcta acaaaacgcc
    20221 ggagatggca aataccgctg tgccgactat atccagccaa tagacgagca ttgttcaatc
    20281 cccactgagc acctaaaaag gtcaggcgct aatctacctg tgaaagcgca ttacagagct
    20341 gttgtgcagc gaggataata cgtgggcttg cacgttcaaa ccagtcactc gtgagaggaa
    20401 taacgggaat tttgagctgt tcaccccagt attgttcgat tttaggaatt tggtccggtc
    20461 cgcctgtaat gacaatcgcc tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg
    20521 gccagggaac ccggctgtct ttaaagatgt tttctccgcc acaaacttcg agtacctggt
    20581 tctgaatcga ctcttttcca ctggtaaatg gcggattaat gccgaattgc agaaaaacac
    20641 gttttttagg tttatcagca tattgcgctt tcaattgcgc gtactgatcc agcagggatt
    20701 gcgcggcttg ttcggcctta tccggttgcg gactccaggg ggccagttga cgtaacgcat
    20761 tggcaatttg ttcaatgctt gtcgcatcga cccacatcac ttttattccc agcgaagcca
    20821 gctggtcaac ctgccgctcg gcattacctc cacgccaggc aatcaccaga tcgggtttca
    20881 gcgcgacaat gcgttccaga ttcatcccct gccaggtgga aacctgctca atcttttgcg
    20941 cttgtggagg atagtcggaa tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg
    21001 caagttcagt gttggcggga gaaagcgtga tgacgcgcgg tgccgcgttg agccacagtg
    21061 gcgcgagaaa agacagggcg accagcgccc tgaacagtga cttagccatg tgccagtttc
    21121 tgcaccagtg actcaaccat caggctggac tgtttagcgg caacagccag gaactcatcg
    21181 aagctaagat gagactgttg atcggccacg tcggagatgg cgcgtacgac aacaaacggg
    21241 acgttgaaat tgtggcagac atgggcgatt gccgtcgctt ccatctctac agcaatggcc
    21301 tgtgggaagt tgtggcggat tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg
    21361 ctaacaatca ggccacgtac agcgttaaga ttcagttcgg caatgcaggc ctcagcggca
    21421 gcgatcagtt tatcgtcagc tttaaagcct gccggacagc ctggtaactg accgtattca
    21481 taaccaaatg ccgtgacatc cgcgtcgtga taacgtgctt cgtccgagac aacgatatcg
    21541 cccactttca acgttggtgc caggccaccg gcagaaccgg tgttaataat cacatctggc
    21601 ttgcagtgtt ccaacagcaa agtggcaccc agcgccgcag cgactttacc gatgcccgat
    21661 ttcagaagcg caacctcggt tccattcagt tggccggtat agatttcgca accgccgaga
    21721 ctgatagttt gacggttttc gattttgtca cgcagcagcg taacttcttc ttccattgca
    21781 ccaatgatgc cgattttcat agatttactc gcgataagcc cgatttgaag gcatagttta
    21841 ccatgcgctt acggggaagc gtatttctca cgcgggagag gacatggcac agattgattt
    21901 ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca
    21961 tgagatcctg cggatcttcg agagcgatcg cgggcgtatc atcaactctc cggcaattcg
    22021 tcgtctgcaa caaaagaccc aggtttttcc actggagcgc aatgccgccg tgcgcacgcg
    22081 tcttacccac tcgatggaag tccagcaggt ggggcgctac atcgccaaag aaattttaag
    22141 ccgtctgaaa gagcttaaat tgctggaagc atacggcctg gatgaactga ccggaccttt
    22201 tgaaagcatt gttgagatgt catgcctgat gcacgatatc ggcaatccgc cgtttggtca
    22261 ttttggcgaa gcggcgataa atgactggtt tcgccagcgt ttatacccgg aagatgccga
    22321 aagccagcct ctgactgacg atcgctgcag cgtggcggca ctacgtttac gggacgggga
    22381 agaaccgctt aacgagctgc ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa
    22441 tgcacaaggc attcgtctgg tgcatacatt gatgcggatg aatctcacct gggcgcaggt
    22501 tggcggtatt ttaaaatata cccgtccagc gtggtggcgt ggcgaaacgc ctgagacaca
    22561 tcactattta atgaaaaagc caggttatta tctttctgaa gaagcctata ttgcccggtt
    22621 gcgtaaagaa cttaatttgg cgctttacag tcgttttcca ttaacgtgga ttatggaagc
    22681 tgccgacgac atctcctatt gtgtggcaga tcttgaagat gcggtagaga aaagaatatt
    22741 taccgttgag cagctttatc atcatttgca cgaggcgtgg ggccagcatg agaaaggttc
    22801 gctcttttcg ctggtggttg aaaatgcctg ggaaaaatca cgctcaaata gtttaagccg
    22861 cagtacggaa gatcagtttt ttatgtattt acgggtaaac accctaaata aactggtacc
    22921 ctacgcggca caacgattta ttgataatct gcctgcgatt ttcgccggaa cgtttaatca
    22981 tgcattattg gaagatgcca gcgaatgcag cgatcttctt aagctatata aaaatgtcgc
    23041 tgtaaaacat gtgtttagcc atccagatgt cgagcagctt gaattgcagg gctatcgggt
    23101 cattagcgga ttattagaga tttatcgccc tttattaagc ctgtcgttat cagactttac
    23161 tgaactggta gaaaaagaac gggtgaaacg tttccctatt gaatcgcgct tattccacaa
    23221 actctcgacg cgccatcggc tggcctatgt cgaagctgtc agtaaattac cgtcagattc
    23281 tcctgagttt ccgctatggg aatattatta ccgttgccgc ctgctgcagg attatatcag
    23341 cggtatgacc gacctctatg cgtgggatga ataccgacgt ctgatggccg tagaacaata
    23401 accaggcttt tgtaaagacg aacaataaat ttttaccttt tgcagaaact ttagttcgga
    23461 acttcgcgct ataaaacgaa tctgaagaac acagcaattt tgcgttatct gttaatcgag
    23521 actgaaatac atgaaaaaaa ccacattagc actgagtgca ctggctctga gtttaggttt
    23581 ggcgttatct ccgctctctg caacggcggc tgagacttct tcagcaacga cagcccagca
    23641 gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt
    23701 agaaggtagc acaaccgtta atacgccgcg tatgccgcgt aatttccagc agttcttcgg
    23761 tgatgattct ccgttctgcc aggaaggttc tccgttccag agctctccgt tctgccaggg
    23821 gggccagggc ggtaatggcg gcggccagca acagaaattc atggcgctgg gttccggcgt
    23881 catcattgat gccgataaag gctatgtcgt caccaacaac cacgttgttg ataacgcgac
    23941 ggtcattaaa gttcaactga gcgatggccg taagttcgac gcgaagatgg ttggcaaaga
    24001 tccgcgctct gatatcgcgc tgatccagat ccagaacccg aaaaacctga ccgcaattaa
    24061 gatggcggat tctgatgcac tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt
    24121 tggtttgggc gagacggtaa cttccgggat tgtctctgcg ttggggcgta gcggcctgaa
    24181 tgccgaaaac tacgaaaact tcatccagac cgatgcagcg atcaaccgtg gtaactccgg
    24241 tggtgcgctg gttaacctga acggcgaact gatcggtatc aacaccgcga tcctcgcacc
    24301 ggacggcggc aacatcggta tcggttttgc tatcccgagt aacatggtga aaaacctgac
    24361 ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag ctgggtatta tggggactga
    24421 gctgaactcc gaactggcga aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag
    24481 ccaggttctg cctaactcct ccgctgcaaa agcgggcatt aaagcaggtg atgtgatcac
    24541 ctcactgaac ggtaagccaa tcagcagctt tgccgcactg cgtgctcaag tgggtactat
    24601 gccggtgggc agcaaactga ccctgggctt gctgcgcgac ggtaagcagg tcaacgtgaa
    24661 tctggaactg cagcagagca gccagaatca ggttgattcc agctccatct tcaacggcat
    24721 tgaaggcgct gagatgagca acaaaggcaa agatcagggc gtggtagtga acaacgtgaa
    24781 aacgggcact ccggctgcgc agatcggcct gaagaaaggt gatgtgatta ttggcgcgaa
    24841 ccagcaggca gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt
    24901 gctggcactg aacattcagc gcggcgacag caccatctac ctgttaatgc agtaatctcc
    24961 ctcaacccct tcctgaaaac gggaaggggt tctccttaca atctgtgaac ttcaccacaa
    25021 ctccatacat cttcatcatc ctttaggcat ttgcacaatg ccgtacgtta cgtacttcct
    25081 tatgctaagc cgtgcataac ggaggactta tggctggctg gcatcttgat accaaaatgg
    25141 cgcaggatat cgtggcacgt accatgcgca tcatcgatac caatatcaac gtaatggatg
    25201 cccgcgggcg aattatcggc agcggcgatc gtgagcgtat tggtgaattg cacgaaggag
    25261 cattgctggt actttcacag ggacgagtcg tcgatattga tgacgcggtg gcacgtcatc
    25321 tgcacggtgt gcggcagggg attaatctac cgttacggct ggaaggtgaa attgtcggcg
    25381 taattggcct gacaggtgaa ccagagaatc tgcgtaaata tggcgaactg gtctgcatga
    25441 cggctgaaat gatgctggaa cagtcgcggt tgatgcactt gttggcgcag gatagccgtt
    25501 tgcgggagga actggtgatg aacctgattc aggcagagga gaatactccc gcacttactg
    25561 aatgggcgca acggctgggg atcgatctca atcaaccgcg agtggtggct attgttgagg
    25621 tcgacagcgg tcagcttggt gtggacagcg caatggcgga gttacaacaa ctgcaaaacg
    25681 cgctgactac gcccgaacgt aataatctgg tggcgattgt ctcgctaacc gaaatggtgg
    25741 tgttgaaacc ggcgttgaac tcttttgggc gctgggatgc agaagatcat cgtaagcgag
    25801 ttgaacaact gattacccgc atgaaagagt acggccagct gcgttttcgc gtttcactgg
    25861 gcaactattt taccggtcct ggcagtattg cccgatccta tcgtacggcg aaaacgacga
    25921 tggtggtggg taaacagcgg atgccagaaa gtcgctgcta tttttatcag gatctgatgt
    25981 tacctgtgtt actcgacagt ttgcgtggcg actggcaggc caacgaactg gcgcgaccgc
    26041 tggcgcggct gaaagcgatg gacaataacg gcttgctgcg acgaacgctg gcggcgtggt
    26101 ttcgtcacaa tgtgcaaccg ctggcaacgt caaaggcgtt gtttattcat cgtaataccc
    26161 tggagtatcg gcttaatcgt atatcggaac tgaccgggct tgatttgggc aattttgatg
    26221 acaggttgct gctgtatgtg gcgttacaac tggatgaaga gcggtaggtt atgcgttaag
    26281 gttgaggagg cgctggctca ttcccctcag cccgaagggg agaggggaat gcccgagtta
    26341 accgtcaatt cgggattatt tacgcgttag tttttccaga tcggcttcaa tctcgctgat
    26401 cttattggtt acgactgact ccaggtgacg taagtcgtcg aggatcttac gcttaagatc
    26461 aacttcgctg cggtcacgct gacagatttg atcaagctca tcaatgatat accgtagatt
    26521 cgggctgatt tcctggactt ctttataacc ctgacccaca ccatcagcga cgaccgtttt
    26581 acgctgacgc ggatatttaa acttaacgct cttggcgaaa aactcgcctt tgtctttctg
    26641 gaaatagatt ttcagaatat cgttgttggc ttcctgccgg aggctgtaac gatcaatttc
    26701 ttcaggattg gtaataccca gacttttcag attgtcgtac atagcgttac ctcaaaatga
    26761 gtcagtaaat tgtgcttatt ttagcatttg gcctggcccg cccggctcga ctgtgatcgc
    26821 agaaagcctt tttcttccag acttctatca tggcgtaaaa atcaaaaaat tacctgcttt
    26881 attgtggcga tattccatgg gatggattag cacatttatc ttcgttttgc gaattacatg
    26941 acagtccgaa acaagatgaa aaagtattta aaaaaggggg aggaagaatg agtaggacga
    27001 attattctga accagaaagc aatcgggcag cgtcgtgctg cccgtgtgca tacttttagt
    27061 cgatggtacg caacagttcg ttaatgccga ctttgccgcg agttttcgcg tcaactttct
    27121 taacgataac cgcacagtaa agactgtatt tgccatcttt cgacggcaga ttgcctgaaa
    27181 caaccacaga ccccgccgga acgcgtccgt agtggacttc gccagtttcg cgatcataga
    27241 tcttggtgct ttgcccaatg taaacaccca ttgagatcac cgaaccttct tctacgatga
    27301 cgccttcaac gatttctgaa cgcgcgccga tgaagcagtt atcttcaata atggtcgggt
    27361 tagcctgcag cggttccagc acaccgccga tgcccacgcc accggaaagg tggacgtttt
    27421 taccaatctg cgcacaagaa ccgacggtcg cccaggtatc gaccatggtg ccttcatcaa
    27481 catatgcgcc gatgttgacg taagacggca tcagcacggt gttacgggca ataaacgcac
    27541 cctgacgtac cgccgcaggt ggtacaacgc ggaagccttc tttctggaaa cgtgcttcgt
    27601 cgtagtcggc gaatttcatc ggcactttgt cgaagtagcg gctttctgcc ccttcgatca
    27661 cctgattatc attaatacgg aaagagagca gcaccgcttt tttcaaccac tgatgcgtca
    27721 cccactgacc gtcaattttt tccgctacac gcagtgcgcc ggaatccagc agggcgatca
    27781 cctgatttac cgcttcgcgg gtaacggtgt ctgcattggc tggcgtgatc tcggcacggc
    27841 gttcaaaagc ggtttcaata atgttctgta actgctgcat tgttaaactc ttttcatatc
    27901 agtaaacaca tcacccttta tcgtttggat tgagggcctc tgtcaaccgc tgatgcactt
    27961 cctgctgcaa ctcgttatta agtgcacgcc ggtcagcggt ggcaattatg aataaatctt
    28021 ctactcgctc gccaatggtt gtaattcggg caccatgaag cgaaattccc agatcggcaa
    28081 aaattttccc gactcgcgcc agcagcccag gttggtcgag ggcgatcagt tcgaggaacg
    28141 atttgcggtc ggtatgggtt ggcaaaaacg tcacttcggt ttcaacagta aaatggcgca
    28201 atttggcggg ttggcgacgg ggctgcggtg gctgccagct actttgtgtc agtacttgct
    28261 ccagaccaaa ccgaataacc tcatggcgat ctgcggacag cgggctgcca tcgggttcca
    28321 gcacgataaa ggtatccatc gccataccgt cgcgagtggt gaatatttgt gcgtcgtgaa
    28381 cgcttaagtt gcgtctatct aactcggcac aaacagcagc aaacaagtaa gggcggtccg
    28441 ggctccagat aaaaatctcg gtgcctccac gcgtagcctg cgggctaagc aataccagcg
    28501 gtttgcttaa atcatgctgt aataaatggc gcgcatgcca ggccagttga tttgggctat
    28561 ggcggacaaa atagttagca cgacagcgtg accaaatttg gtgcagcgcc tcttcgtcga
    28621 tgttatccat gcgcagcagt gccagcgcct ggagttggtg atggcgaacc cgttcgcgca
    28681 tatccggcgt gttttgcatt ccgcgtcgta gctgtttttc ggtggcgaaa tagagttcgc
    28741 gcaacagact ttgcttccag ctattccaca gcgtttcgtt ggtggcgcaa atgtcagcca
    28801 ctgtcaggca taccagatag cgcagacgat tttccgtttg aacttcttcg gcaaactgct
    28861 tgatgacttc cgggtcctga atatcgcgac gctgagctgt caccgacatc agcaagtgtt
    28921 ggcgaaccag ccaggcgacc agctgtgttt cgcgtgagtt cagcccgtgg agttcggcaa
    28981 aatgcactac atcctgagca ccgagaatgg aatggtcgcc gccgcgtcct ttggcgatat
    29041 cgtgaaacag cgcggcgatg aaaatcagct cagttgacgg taggcgcggc cagacgtcca
    29101 cacacaacgg atggcgctgg cgcgtttctt cactggcaaa actctccagt ttcagcatca
    29161 cgcggatagt atgttcatcc accgtgtagg cgtggaacag atcaaactgc atctgcccga
    29221 cgatatgtga ccattgcggc atatacgcgc cgagtacgct atggcgatgc attggcaata
    29281 gcccgcgccg caccgctccg gggtgacgca gaatgctcaa aaacagtttt cgtgcttccg
    29341 gaatattaca cagcggttgt tgcagatggc gacgggcatg gcgtaactgg cgcagtgtgg
    29401 tggagtaaat gccggtgatc gcactgttgc gcaccatggt gtagaacata cgcaagatgg
    29461 cttccggctg gcgcataaat agtgtttcat cacgcaggtc gattagcgta ccgcgtagct
    29521 gaaactcatc gtcgattgga cgtggttttt cgtcggcggg aagggcgagg atggcttcat
    29581 cgaacagttg cagcagcatc tggttgagtt cactgacgcg gcgtgtcacg cggaagtaat
    29641 ccttcatcat ccgctcgacc ggttcgttac cttcgccact gtaattcaga cgctgggcga
    29701 cgctaagctg gcgatcgaat aacaggcgat tatcgtaacg gctgacgacc agatgcaggg
    29761 caaagcgaat acgccacaat atatgcagac attcgtttaa ttccgcccgc tccgctgagg
    29821 ttaagaagcc aaacccaacc atttcatcca gcgatgttgc gccaaaatga cggcgggcca
    29881 cccattgcag agtgtggata tcgcgcaagc cgccagggct acttttgatg tctggttcaa
    29941 gattgtagct ggtgccgtgg taacgctgat ggcgctggtt ctgttcttca actttcgccg
    30001 cgtagaactt gtcggaaggc cagaatcctt cgctgaaaat atgtttttgc agttcaagaa
    30061 atagcgcaac atcgccaatt aataagcggg attcgatcaa attggtggcg acggttaaat
    30121 ccgataaccc ttccagcatg cactcttcaa gcgtgcgcac actatgaccg acttccagct
    30181 ttacatccca gagcagcgtt aacagctcgc ccactttttg cgcctgatcg tccgggagct
    30241 ttttacggct taaaatcagt aaatcgatgt cagaaagtgg atgcagctcg ccacgaccgt
    30301 agccaccgac ggcgaccaat gccaggtcgg caatctggct gaatcccgct tcaatccata
    30361 atcgttgcag gagctggtcg ataaactcgg tgcgcgcctc aatcaactgt tctgcagaga
    30421 tcccattgtc aaaggcatca cccagccaac gctggaaagt atcgatatgg gcttttatcc
    30481 caccgacggt taattcatca cggggccaga cgcatggatt ttgcggttga ccgggcaggg
    30541 tggggagagc ggtgtttgcg tactgttctg gaagggtatt cattgtgcgc cacccataag
    30601 attaaattat cgcattaaaa aagccggcat tcgccggctt cattttattc gtcgtgcgag
    30661 attatcgccg ggatggtgtc atccttgcgt agtgtcagaa tttcgcagcc gttatcagtc
    30721 accacaatag tatgctcata ttgtgcagac aagctgcgat ctttggtttt taccgtccag
    30781 ccatctttca tggtgcggat ctctttttta cccgcgttga ccattggctc gatggtgaac
    30841 gtcatcccag gtttcagtac gacgttggtt tcacgggagt catagtgcag cacctgcggt
    30901 tcttcgtgga agccgcgacc aataccgtgt ccgcaatatt cacgaacgac ggagaagcct
    30961 tctgcttcga caaatttctg aatcgccgca ccgatttcgc gcagattaat gcctggtttt
    31021 accatgcgta gcgccaggta caggctttct tgcgtgatac ggcacagacg ttcgcccatg
    31081 atggtcggct taccgacgat aaacattttc gaggtatcgc cgtggaaacc atctttgatt
    31141 acggtgacat caatgttaac gatatcgcca tctttcagca gcttcgcatc gtccgggata
    31201 ccgtggcaca ccacttcatt aattgagatg caaacggatt tcggataacc gtgatagccg
    31261 aggcaggcag aaaccgcgtg ttgttcatta acaatgtaat cattacagat gcgatccagc
    31321 tcgccggtgc tgacgcccgg tttaacatac ggttcgatca tctccagcac ttcggcagcc
    31381 agtcggccag cgacgcgcat tttttcgata tcttctgggg tcttgattga gatagccatt
    31441 aattctgtcc atcagcgtcg gtgataccga caatatatat gtaagtgccg tcaatggtat
    31501 cacaccctgg caaattgaga atcattctga atttcgccaa acgtgccact gaaggttttc
    31561 tataatagaa aattcgacgt ctgatgctgt acacagcgcc aacaattatt ggtgtccacg
    31621 gcgtatttgt ggtataaagc gcgccggact tccgatccat ttcgtataca cagactggac
    31681 ggaagcgaca atctcacttt gtgtaacaac acacacgtat cggcacatat tccggggtgc
    31741 cctttggggt cggtaatatg ggatacgtgg aggcataacc ccaactttta tatagaggtt
    31801 ttaatcatgg caactgtttc catgcgcgac atgctcaagg ctggtgttca cttcggtcac
    31861 cagacccgtt actggaaccc gaaaatgaag ccgttcatct tcggtgcgcg taacaaagtt
    31921 cacatcatca accttgagaa aactgtaccg atgttcaacg aagctctggc tgaactgaac
    31981 aagattgctt ctcgcaaagg taaaatcctt ttcgttggta ctaaacgcgc tgcaagcgaa
    32041 gcggtgaaag acgctgctct gagctgcgac cagttcttcg tgaaccatcg ctggctgggc
    32101 ggtatgctga ctaactggaa aaccgttcgt cagtccatca aacgtctgaa agacctggaa
    32161 actcagtctc aggacggtac tttcgacaag ctgaccaaga aagaagcgct gatgcgcact
    32221 cgtgagctgg agaaactgga aaacagcctg ggcggtatca aagacatggg cggtctgccg
    32281 gacgctctgt ttgtaatcga tgctgaccac gaacacattg ctatcaaaga agcaaacaac
    32341 ctgggtattc cggtatttgc tatcgttgat accaactctg atccggacgg tgttgacttc
    32401 gttatcccgg gtaacgacga cgcaatccgt gctgtgaccc tgtacctggg cgctgttgct
    32461 gcaaccgtac gtgaaggccg ttctcaggat ctggcttccc aggcggaaga aagcttcgta
    32521 gaagctgagt aataaggcac gctcgtacga gcccttatag accaggtagt acacgtttgg
    32581 ttagggggcc tgcatatggc cccctttttc acttttatat ctgtgcggtt caatgccggg
    32641 cagatcacat ctccgaggat tttagaatgg ctgaaattac cgcatccctg gtaaaagagc
    32701 tgcgtgagcg tactggcgca ggcatgatgg attgcaaaaa agcactgact gaagctaacg
    32761 gcgacatcga gctggcaatc gaaaacatgc gtaagtccgg tgctattaaa gcagcgaaaa
    32821 aagcaggcaa cgttgctgct gacggcgtga tcaaaaccaa aatcgacggc aactacggca
    32881 tcattctgga agttaactgc cagactgact tcgttgcaaa agactctggt ttccaggcgt
    32941 tcgcagacaa agttctggac gcagctgttg ctggcaaaat cactgacgtt gaagttctga
    33001 aagcacagtt cgaagaagaa cgtgttgcgc tggtagcgaa aattggtgaa aacatcaaca
    33061 ttcgccgcgt tgctgcgctg gaaggcgacg ttctgggttc ttatcagcac ggtgcgcgta
    33121 tcggcgttct ggttgctgct aaaggcgctg acgaagagct ggttaaacac atcgctatgc
    33181 acgttgctgc aagcaagcca gaattcatca aaccggaaga cgtatccgct gaagtggtag
    33241 agaaagaata ccaggtacag ctggatatcg cgatgcagtc tggtaagccg aaagaaatcg
    33301 cagagaaaat ggttgaaggc cgcatgaaga aattcaccgg cgaagtttct ctgaccggtc
    33361 agccgttcgt tatggaacca agcaaaactg ttggtcagct gctgaaagag cataacgctg
    33421 aagtgactgg cttcatccgc ttcgaagtag gtgaaggcat cgagaaagtt gagactgact
    33481 ttgcagcaga agttgctgcg atgtccaagc agtcttaatt atcgaaaagg agccgcctga
    33541 gggcggcttc tttttgtgcc catcttgtaa attcagctaa cccttgtggg gctgcgctga
    33601 aaagcgacgt acaatgtcgc tagtattaat tcatttcaat cgttgacagt ctcaggaaag
    33661 aaacatggct accaatgcaa aacccgtcta taaacgcatt ctgcttaagt tgagtggcga
    33721 agctctgcag ggcactgaag gcttcggtat tgatgcaagc atactggatc gcatggctca
    33781 ggaaatcaaa gaactggttg aactgggtat tcaggttggt gtggtgattg gtgggggtaa
    33841 cctgttccgt ggcgctggtc tggcgaaagc gggtatgaac cgcgttgtgg gcgaccacat
    33901 ggggatgctg gcgaccgtaa tgaacggcct ggcaatgcgt gatgcactgc accgcgccta
    33961 tgtgaacgct cgcctgatgt ccgctattcc attgaatggc gtgtgcgaca gctacagctg
    34021 ggcagaagct atcagcctgt tgcgcaacaa ccgtgtggtg atcctctccg ccggtacagg
    34081 taacccgttc tttaccaccg actcagcagc ttgcctgcgt ggtatcgaaa ttgaagccga
    34141 tgtggtgctg aaagcaacca aagttgacgg cgtgtttact gctgatccgg cgaaagatcc
    34201 aactgcaacc atgtacgagc aattgactta cagcgaagtg ctggaaaaag agctgaaagt
    34261 catggacctg gcggccttca cgctggctcg tgaccataaa ttaccgattc gtgttttcaa
    34321 catgaacaaa ccgggtgcgc tgcgccgtgt ggtaatgggt gaaaaagaag ggactttaat
    34381 cacggaataa ttcccgtgat ggataaataa gggtaagatt ccgcgtaagt atcgcggggg
    34441 atcttacccg taatatgttt aatcagggct atacttagca cacttccact gtgtgtgact
    34501 gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga tatcagaaaa
    34561 gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat cagcaaaata
    34621 cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata ttacggcacg
    34681 ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac tctgaaaatc
    34741 aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc gtccgatctt
    34801 ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc gctgacggaa
    34861 gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc gcgtgttgca
    34921 gtacgtaacg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa agataaagag
    34981 atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac tgatgctgca
    35041 atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca gttctgattt
    35101 cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg cgttttgctt
    35161 tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg gctcgaccgg
    35221 ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact tccgcgtagt
    35281 tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg aattctctcc
    35341 ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga tgctacagca
    35401 acagggtagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata tggcagcgct
    35461 tgaggatgtt gatcaggtga tggcagccat tgttggcgct gctgggctgt tacctacgct
    35521 tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac tggttacctg
    35581 cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac cggtcgatag
    35641 cgaacataac gccatttttc agagtttacc gcaacctatc cagcataatc tgggatacgc
    35701 tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg gccctttccg
    35761 tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat caagcctgcc gtcatccgaa
    35821 ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca aaggtctgga
    35881 atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag tgctgattca
    35941 cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc tggcgcagct
    36001 gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga atcgcgtgaa
    36061 ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg ccgcaccgga
    36121 ttatgatcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag gccaggcagc
    36181 gacgacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg cgcaacaaat
    36241 ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg atatgcgcga
    36301 accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgcgtgaag tcgccagaaa
    36361 agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc gctatttgtt
    36421 agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg gctttataag
    36481 gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta ttcctgagta
    36541 ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca accacttagc
    36601 gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg caatggtcgc
    36661 tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc aaaatccgtc
    36721 cgccgggctg tctcttttgc ggccaacaac ggtattgagg cgttaacgct gtatgccttt
    36781 agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga actgtttgtg
    36841 tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct gcgtattatt
    36901 ggcgatacca gtcgctttaa ctcgcgtttg caagaacgta ttcgtaaatc tgaagcgcta
    36961 acagccggga ataccggtct gacgctgaat attgcggcga actacggtgg acgttgggat
    37021 atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct gcaaccagat
    37081 cagatagatg aagagatgct aaaccagcat gtctgtatgc atgaactggc ccctgtagat
    37141 ttagtaatta ggactggggg ggagcatcgc attagtaact ttttgctttg gcaaattgcc
    37201 tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca agactttgaa
    37261 ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga gcccggtgat
    37321 gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc ttttgtgtta
    37381 atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc cattgtaacg
    37441 ctggtggtct gcatgctggc agcgtgggaa tggggacagc ttagcggttt taccactcgt
    37501 tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat gctttttctg
    37561 ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact ttgggcttcg
    37621 ctgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc cgcagcaatc
    37681 tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt tcccttcttc
    37741 tggggcatgc tggcgttacg ggcctggcac tacgacgaga atcattacag tggcgcaata
    37801 tggctgctct atgtcatgat cctggtatgg ggcgctgact ctggcgcata tatgtttggc
    37861 aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac ctggcaaggc
    37921 tttataggtg gactcgcgac cgcagcagta atctcatggg gttatggcat gtgggcgaat
    37981 ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt agcctcagtg
    38041 ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga cagcggtcat
    38101 ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc tgcggtaccg
    38161 gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt aatatgctga
    38221 gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc accgtgcatg
    38281 aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt ttctcaatag
    38341 ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat gttatcgccc
    38401 tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg gtcgttccgg
    38461 aactccgcca ccatgccttc aataataaat ctgtcggcca acgagcggcg attattgccg
    38521 caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt tttattattg
    38581 gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata gctgcggaag
    38641 cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg cctgattggg
    38701 atgccgtgcg tttgcagttg gtcgataaaa ttggtgatga aagcaccacc attacagtag
    38761 cgccatttgg cagcgaccaa cggcgggatg taaagctcga tttacgtcac tgggcgtttg
    38821 agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg ccgcaaattg
    38881 aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt ttgcaagcag
    38941 gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc tttgtgatgc
    39001 ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag gggagtccct
    39061 tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt ggttttgtcg
    39121 gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc cagtatgggc
    39181 cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag ctgacggtca
    39241 gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt gggccgatct
    39301 ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac ctgccgtttc
    39361 ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc gtacttgacg
    39421 gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta tccgagcggg
    39481 ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg gggcttgcac
    39541 ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa taacgatggc
    39601 gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat acggtgctga
    39661 agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg ttggtgcggc
    39721 cctcctcagt atgccggtgc gcacaggcga cacggttaat gatgaagata tcagtaatac
    39781 cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc gtgatggtga
    39841 tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt tctccggtaa
    39901 caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggtg tgcgtgtggg
    39961 cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag acttctacta
    40021 cagcgtcggt aaatatagcg ccagcgtaaa agctgtcgtg accccgctgc cgcgcaaccg
    40081 tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc aaattaacat
    40141 tgttggtaac catgctttca ccaccgacga actgatctct catttccaac tgcgtgacga
    40201 agtgccgtgg tggaacgtgg taggcgatcg taaataccag aaacagaaac tggcgggcga
    40261 ccttgaaacc ctgcgtagtt actatctgga tcgcggttat gcccgtttca acatcgactc
    40321 tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga acatcaccga
    40381 aggcgatcag tacaagcttt ctggcgttca agtgagcggc aaccttgccg ggcactccgc
    40441 tgaaattgag cagctgacta agatcgagcc gggcgagctg tataacggca ccaaagtgac
    40501 caagatggaa gatgacatca aaaagcttct cggtcgctat ggttatgcct atccgcgcgt
    40561 acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg tgaacgttga
    40621 tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata cctcgaaaga
    40681 tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggctgggaa gcgatctggt
    40741 cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg ataccgatac
    40801 ccaacgtgtt ccgggtagcc cggaccaggt agatgtagtc tacaaggtga aagaacgtaa
    40861 caccggtagc ttcaacttcg ggattggtta cggtactgaa agtggcgtga gcttccaggc
    40921 tggcgtgcaa caggataact ggttaggtac aggttatgct gttggtatca acgggaccaa
    40981 aaacgattac cagacctatg ctgaattgtc ggtaaccaac ccgtacttca ctgtagatgg
    41041 tgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg ccgacctgtc
    41101 cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga tcaacgaata
    41161 taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca tgcagcctca
    41221 ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct ctgatcagga
    41281 taacagcttc aaaacggacg acttcacgtt caactatggt tggacctata acaagcttga
    41341 ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag tgaccattcc
    41401 tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg tgccgatcga
    41461 tgacgatcac aaatgggttg ttctggggcg tacccgctgg ggttatggtg atggtttagg
    41521 cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca ccgtgcgtgg
    41581 cttccagtcc aataccattg gtccgaaagc agtttacttc ccgcatcagg ccagtaatta
    41641 tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc tgtgtaaatc
    41701 ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca tcaccccgac
    41761 gccgtttatt agcgataagt atgctaactc ggttcgtact tccttcttct gggatatggg
    41821 taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg actatagtga
    41881 tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc cattggggcc
    41941 gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg cagaacagtt
    42001 ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta gtggtagtgt
    42061 agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac tgcaccctcc
    42121 ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct gcaggtctcg
    42181 gtttagcact ggcaacatct gctcaggcgg ctgacaaaat tgcaatcgtc aacatgggca
    42241 gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa aatgagttca
    42301 aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa atgaaaaagc
    42361 tgcagtccat gaaagcgggc agcgatcgca ctaagctgga aaaagacgtg atggctcagc
    42421 gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt cgttccaacg
    42481 aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt gccaacagcc
    42541 aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc gatgtaaaag
    42601 acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt cgactggctg
    42661 atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc atcaccggcg
    42721 ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac ccaaaatacc
    42781 gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac gatcttcctt
    42841 tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg cgcatggcgc
    42901 aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg gtgatcgacg
    42961 cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt gagtccggcg
    43021 ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa aacagcaaaa
    43081 tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc cagatcggtc
    43141 agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt tatgccaacg
    43201 atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc gatcgcgtgg
    43261 agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactatt attggcaatg
    43321 gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc gacaatacgg
    43381 cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac tgcatgatcg
    43441 gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg gttacgggca
    43501 tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc attccgctgc
    43561 aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat gacatgagca
    43621 agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc atcttttgtt
    43681 cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt attattgtcg
    43741 tttcttatat tttgacagga agagtatctt gactactaac actcatactc tgcagattga
    43801 agagatttta gaacttctgc cgcaccgttt ccctttctta ctggtggatc gcgtgctgga
    43861 ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg agccattctt
    43921 ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg aagcaatggc
    43981 acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg gtgagctgta
    44041 ctatttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg gcgatcaaat
    44101 gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta aaggggttgc
    44161 tctggtcgac ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta gccgggaggc
    44221 ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg aagagggcgc
    44281 gtcgattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc atgtcgaaat
    44341 tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggccatacta aaattggccg
    44401 cgataatgag atttatcagt tcgcctccat cggtgaagtt aaccaggatc tgaaatatgc
    44461 tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa gcgtcaccat
    44521 tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca acttactgat
    44581 gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc tcgccaacaa
    44641 cgcaacgctg gcgggtcacg tatcggtaga cgacttcgcg atcatcggcg gcatgaccgc
    44701 agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggttgct ccggtgtggc
    44761 gcaggacgtc cctccttatg tcattgcgca gggtaaccac gcaacgccgt tcggtgtcaa
    44821 tatcgaaggg ctgaagcgcc gcggattcag ccgcgaggcg attaccgcta tccgcaatgc
    44881 gtataagctg atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg aaattgctga
    44941 actggcggaa acgtatccgg aagtgaaagc ttttaccgat ttctttgcac gctcaacgcg
    45001 cggtctgatt cgttaatgac tgaacagcgt ccattaacga ttgccctggt cgccggagaa
    45061 acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg tgtgcccaac
    45121 gcccgctttg ttggcgttgc cgggccacga atgcaggctg aaggctgcga agcctggtac
    45181 gaaatggaag agttggcggt gatgggcatt gttgaagtgc tcggtcgtct gcgtcgctta
    45241 ctgcatattc gtgccgatct gacaaagcgt tttggtgaac taaagccaga tgtttttgtt
    45301 ggcattgatg cgcctgactt caatattacc ctcgaaggta accttaaaaa gcagggtatc
    45361 aaaaccattc attatgtcag tccgtccgtc tgggcgtggc gacagaaacg cgttttcaaa
    45421 ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc gttttatgac
    45481 aagtataacg tgccgtgccg ttttatcggt cacacgatgg ctgatgccat gccgttagat
    45541 ccagataaaa acgccgcccg tgatgtgctg ggtatccctc acgatgccca ctgtctggca
    45601 ttgttgccgg gcagccgtgg tgcggaagtc gagatgctga gcgccgattt cctgaaaacg
    45661 gcccagcttt tgcgccagac gtaccccgat ctggagatcg tggtgccgct ggtgaatgcc
    45721 aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct ttcagttcat
    45781 ttgctggatg ggatgggccg tgaggcgatg gtcgccagcg atgcggcact actggcgtcg
    45841 gggacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg atatcgcatg
    45901 aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgaatatgt ctcgctgcca
    45961 aatctgctgg cgggcagaga gttagtcaaa gaattattgc aggaagagtg tgagccgcaa
    46021 aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca cgcaatgcac
    46081 gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca ggcggcacaa
    46141 gccgttctgg agttagcaca atgatcgaat ttgtctatcc gcacacgcag ctggttgcgg
    46201 gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct gcggtgatcc
    46261 ttgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc gaaaaacgcc
    46321 gtctggcgct ctatgaagag atcaaagaga aagcgttgag ctggagtctg ggccgcgcgg
    46381 aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg atgcagcgtg
    46441 ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac cgctgcccga
    46501 aattaccgat gcctgcgatg gctgtggtga aaggcgatag ccgcgtaccg gaaatcagtg
    46561 ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg gatattgttt
    46621 tcccgcaata tggttttgcc caacacaaag ggtatccaac cgcttttcat ctggaaaaac
    46681 tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc aaacgagcac
    46741 tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg aagatgtctg
    46801 aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat ggcctggcca
    46861 aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg gcgatcaccg
    46921 atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc gcagggatta
    46981 agcctatcgt cggggcagat tttaacgtcc agtgcgacct gttgggtgat gagttaaccc
    47041 acctgacggt actggcggcg aacaataccg gctatcagaa tctgacgttg ctgatctcaa
    47101 aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac tggcttatcg
    47161 aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc ggacgcagtc
    47221 ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa gaacacttcc
    47281 cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa agctatctgc
    47341 acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgact aacgatgtgc
    47401 gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc cacgacggct
    47461 ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat atgcgtagcg
    47521 aagaggagat gtgcgagctg tttgccgaca tccccgaagc tcttgccaac accgttgaga
    47581 tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata cttcctgccg cagttcccga
    47641 ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagagggc ctggaagagc
    47701 gtctggcctt tttattccct gatgaggaag aacgtgttaa gcgccgcccg gaatatgacg
    47761 aacgtctgga gactgaactt caggttatca accagatggg cttcccgggc tacttcctta
    47821 tcgttatgga atttatccag tggtcgaaag ataacggtgt accggtaggg ccaggccgtg
    47881 gctccggcgc aggctcactg gtggcctacg cgctgaaaat caccgacctc gatccgctgg
    47941 aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg cctgacttcg
    48001 acgttgactt ctgtatggag aaacgcgatc aggttatcga acacgtagcg gacatgtacg
    48061 gtcgtgatgc ggtatcgcag attatcacct tcggtacgat ggcggcgaaa gcggtgatcc
    48121 gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgatcgtatc tcgaaactga
    48181 tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg cagctgccgg
    48241 aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc aaactggaag
    48301 gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg accaaaatta
    48361 ccgattttgc gccgctttat tgcgatgaag agggcaaaca tccggtcacc cagtttgata
    48421 aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg cgtacgctca
    48481 ccatcatcaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat ggcgagccgc
    48541 cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg ctgcaacgct
    48601 cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg atcaagcgtc
    48661 tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc cctggtccgt
    48721 tgcagtcagg gatggtggat aactttattg accgtaaaca tggtcgcgaa gaaatctcct
    48781 atccagacgt acagtggcag catgaaagcc tgaaaccggt actggagcca acctacggca
    48841 ttatcctgta tcaggaacag gtcatgcaga ttgcgcaggt gctttctggt tataccctcg
    48901 gtggcgcgga tatgctgcgt cgtgcgatgg gtaagaaaaa gccggaagag atggcgaagc
    48961 agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa ctggcgatga
    49021 aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg cactctgcgg
    49081 cctatgcttt ggtgtcatat caaacattat ggctgaaagc gcactatccg gcagagttta
    49141 tggcggcggt aatgaccgcc gatatggaca acaccgagaa ggtggtgggc ctggtggatg
    49201 agtgctggcg gatggggctg aaaatcctgc caccagatat aaactccggt ctttaccatt
    49261 tccacgtcaa cgacgacggc gaaatcgtgt atggtattgg cgcgatcaaa ggggtaggtg
    49321 aaggtccgat tgaggcgatc atcgaagccc gtaataaagg cggctacttc cgcgaactgt
    49381 ttgatctctg cgcccgaacc gacaccaaaa agttaaaccg gcgggtgttg gaaaaactga
    49441 ttatgtccgg ggcgtttgac cgtcttgggc cacaccgcgc agcgctgatg aactcgctgg
    49501 gcgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agccatcggt caggccgata
    49561 tgttcggcgt gctggccgaa gagccggaac aaattgaaca atcctacgcc agctgccaac
    49621 cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggtctg tacctgacgg
    49681 gacaccctat caaccagtat ttgaaagaga ttgagcgtta tgtcggaggc gtaaggctga
    49741 aagacatgca cccgacagaa cgtggtaaag tcatcacggc tgcggggctc gttgttgccg
    49801 cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg gatgaccgtt
    49861 ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag caattgctgg
    49921 aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc agcggtgggc
    49981 ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa tatgctcgcg
    50041 ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac cgactccgtc
    50101 agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat cagagggcgg
    50161 atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc gatcgtttat
    50221 taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt gactaataca
    50281 ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag ctggaagcga
    50341 aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt aacatcgatg
    50401 aagaagtgca tcgtctgcgt gaaaaaagtg tagaactgac gcgcaaaatc ttcgccgatc
    50461 tcggtgcatg gcagattgcg caactggcac gccatccaca gcgtccttat accctggatt
    50521 acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgcgcg tatgcagacg
    50581 ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg atcattggtc
    50641 atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg ccagcaccag
    50701 aaggttaccg caaagctctg cgcctgatgc aaatggctga acgctttaag atgccaatca
    50761 tcacctttat cgacaccccg ggggcttatc cgggcgtggg cgcagaagaa cgcggtcagt
    50821 ctgaagctat tgcacgcaac ctgcgtgaaa tgtctcgcct cggtgtaccg gtagtttgta
    50881 cggttatcgg tgaaggtggt tctggcggcg cgctggcgat tggcgtgggc gataaagtga
    50941 atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg tccattctgt
    51001 ggaagagcgc cgacaaagca ccgctggcag ctgaagcgat gggtatcatc gctccgcgtc
    51061 tgaaagaact aaaactgatc gactccatca tcccggaacc gctgggtggt gctcaccgta
    51121 acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg gccgatctcg
    51181 acgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg agctacggtt
    51241 acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt ttatcgccac
    51301 ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga acatcattgc
    51361 cattatggga ccgcatggcg tcttttataa agatgagccc attaaagaac tggagtcggc
    51421 gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt tgctgaaatt
    51481 tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgagt acagtctcga
    51541 tttatgtagc gatatcaatc agcttaatga atatctcccg ctttatgcct tcatcaacac
    51601 ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctggt tttttgaata
    51661 tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacaccg acgaatatct
    51721 tgataacatt accccaccgt tcacgaaagc cttgtttacc tacgtcaaag agcggaagta
    51781 caccttttgt acgccgggtc atatgggcgg caccgcatat caaaaaagcc cggttggctg
    51841 tctgttttat gactttttcg gcgggaatac tcttaaggct gatgtatcta tttcggtcac
    51901 cgagcttggt tcgttgctcg accacaccgg gccacacctg gaagcggaag agtacatcgc
    51961 gcggactttc ggcgcggaac agagttatat cgttaccaac ggaacatcga cgtcgaacaa
    52021 aattgtgggt atgtacgccg cgccatccgg cagtacgctg ttgatcgacc gcaactgcca
    52081 taaatcactg gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc tgaaaccgac
    52141 gcgtaatgcg ttggggattc tgggtgggat cccgcgccgt gaatttactc gtgacagcat
    52201 cgaagagaaa gtcgctgcca ccacgcaggc acaatggccg gttcatgcgg tgattaccaa
    52261 ctcaacctat gatggcttgc tctacaacac cgactggatc aaacagacgc tggatgtccc
    52321 gtcgatccac ttcgattccg cctgggtgcc gtacacccat tttcatccga tctaccaggg
    52381 gaaaagtggt atgagcggcg agcgtgttgc gggaaaagtg atcttcgaaa cgcaatcgac
    52441 ccacaaaatg ctggcggcgt tatcgcaggc ttcgctgatc cacattaaag gcgagtatga
    52501 cgaagaggcc tttaacgaag cctttatgat gcataccacc acctcgccca gttatcccat
    52561 tgttgcttcg gttgagacgg cagcagcgat gctgcgtggt aatccgggta aacggctgat
    52621 taaccgttcg gtggagcggg cgctgcattt tcgcaaagag gtccagcggt tgcgggaaga
    52681 gtctgacggt tggtttttcg atatctggca accgccgcag gtggatgaag ccgaatgctg
    52741 gcccgttgcg cctggcgaac agtggcacgg ctttaacgat gcggatgccg atcatatgtt
    52801 tctcgatccg gttaaagtca ctattttgac accggggatg gacgaacagg gcaatatgag
    52861 tgaggagggg atcccggcgg cgctggtggc aaaattcctc gacgaacgtg ggatcgtagt
    52921 agagaaaacc ggcccttata acctgctgtt tctctttagt attggcatcg ataaaaccaa
    52981 agcaatggga ttattgcgtg ggttgacgga atttaagcgc tcttacgatc tcaacctgcg
    53041 gatcaaaaat atgctgcccg atctctatgc tgaagatccc gatttctacc gcaatatgcg
    53101 tattcaggat ctggcgcaag ggatccataa gctgattcgt aaacacgatc ttcccggttt
    53161 gatgttacgg gcattcgata ctttgccgga gatgatcatg acgccacatc aggcatggca
    53221 gagacagatt aaaggtgaag tagaaaccat tgcgctggaa caactggtcg gtagagtatc
    53281 ggcaaatatg atcctgcctt atccaccggg cgtaccgctg ttgatgcctg gagaaatgct
    53341 gaccaaagag agccgcacag tactcgattt tctactgatg ctttgttccg tagggcaaca
    53401 ttaccccggt tttgaaacgg atattcacgg tgcgaaacag gacgaagacg gcgtttaccg
    53461 cgtacgagtc ctaaaaatgg cgggataact tgccagagcg gcttccgggc gagtaacgtg
    53521 ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac aggttcacca tattgcgatt
    53581 attgcgacgg attatgcggt gagcaaagct ttctactgcg atattcttgg tttcacgctg
    53641 caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag gggatttggc gcttaatggg
    53701 caatatgtga ttgagctttt ctcatttccg ttcccgccgg agcgccccag ccgaccggaa
    53761 gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca tcgatgcggc agtggcgcac
    53821 cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac gcaaaaacgc
    53881 ttcaccttct ttaacgatcc ggacgggctg ccgttggaac tgtatgagca gtaaggcttg
    53941 tcatcgcggc atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc gcaccatcat
    54001 gacactcacg ctcaatagac aacttctcac ctcacgccag attctggtgg cctttagcgg
    54061 cgggcttgac tccaccgttc tgctgcatca gttggtgcag tggcggacgg aaaatccggg
    54121 cgtcactcta cgcgctatcc atgtgcatca cggtttaagt gccaatgccg atgcctgggt
    54181 taggcattgc gagaacatct gccaacagtg gcaggtgccg ctggtggtcg agcgcgtgca
    54241 acttgcgcaa gaaggactgg gcattgaggc ccaggcgcgg caggcgcgtt atcaggcatt
    54301 tgcccgcacc ttgttgcccg gtgaagtgct ggtcaccgcg caacatctcg acgatcaatg
    54361 tgaaaccttt ctgctggcgc taaaacgtgg tagcggccct gccgggcttt cggctatggc
    54421 ggaagtttcg gagtttgccg gaacacggct tatccgcccg ttgctcgccc gcacgcgggg
    54481 ggaactggcg cagtgggcat tagcgcatgg tttacgctgg attgaagacg aaagtaatca
    54541 ggacgactca tacgatcgta actttctgcg cctgcgtgta gtgccgttat tgcagcagcg
    54601 ttggccgcat tttgccgaag caacggctcg cagcgccgca ctttgtgctg aacaagagag
    54661 cctgctggat gaattgctgg cagatgattt agcgcactgc caaacgccgc aggggacgct
    54721 gcagattgcg ccaatgctgg cgatgagtga tgcccgtcgc gcggcaatta tccgccgctg
    54781 gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg ctggtgcgga tctggcagga
    54841 agtggcgctg gcgcgggaag atgcctcccc ctgtttacgc tttggcgcgt ttgaaatacg
    54901 ccgctatcaa tcgcaactgt ggtggattaa atccgttgcc gggcaaagcg aaaccattgt
    54961 gccgtggcag acgtggctac aaccgctgga attaccggcg gggctgggaa gtgtacagct
    55021 tactgcggga ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg tgcgtttcaa
    55081 agcgccagga ttgctgcata ttgtcggtcg caacggcgga cgtaagctga agaaaatctg
    55141 gcaggagctg ggcgtgccgc cgtggctacg tgacaccacg ccactgctgt tttatggcga
    55201 aacgctgatt gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg aaggtgagaa
    55261 gggcgtaagt tttgtctggc agaaaacgct tagttaagtg aaagccggat aagacgcatc
    55321 aaacgtcgca tccggcaaaa gtcaatcagg attcgcttac caccaccgta ccgatttccg
    55381 ggtggctaaa gctggtaatt ttatccagac gtagctcacg ggtttcgcca gcggcctcga
    55441 cgaccagata ctccacattt ttgcgggaga ctaaatcgct ggcttttgcc tgcaattttt
    55501 cgccatcttt cagctcaagt gtcagcatta aatgatgctg gcaggcgagc tcaagattat
    55561 cgtaatcatc acaattgatt ggttgatacg tatcattcat tgacataatc gctcaccagt
    55621 aagtttgccg cagcgtatgc tgctttttcc ctgacagcct cagaaagggc gtcgtcggca
    55681 gccatttcat tcagcacttt caaaacgcag cccagcgcgt ccggaacgta tcctaagtct
    55741 ccgctggcga tttccgcgta ccgcttgcgt attaactcac aatatttttc cacatgccct
    55801 cctgtcagca ctctgactta accgtggatg caagtctaag cctacgaaga taaactctgt
    55861 ttcgcaaggt gactatacca cactcatttc tgcaatatca gcgccgcaac tgcacgtatt
    55921 ccgttacaat ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa gcgacaattt
    55981 ataaagcgac ggttaatgtg gccgatctcg accgcaacca gtttctcgat gcctctctga
    56041 cgctggcgcg ccacccttca gaaacccagg agcgtatgat gctgcgcttg ctggcgtggt
    56101 tgaaatatgc tgatgaacgt ctgcaattta cccgtggttt gtgtgccgat gatgagccgg
    56161 aagcgtggct gcgtaacgat cacctgggca ttgatttgtg gattgagctg gggctgccgg
    56221 atgagcggcg gattaagaaa gcctgcaccc aggccgcaga agtggcgctg tttacctata
    56281 atagtcgggc ggcgcaaatc tggtggcagc aaaatcagag caaatgtgtg cagtttgcca
    56341 atctttccgt ctggtatctg gacgatgaac aactggcgaa agtaagcgcc tttgccgatc
    56401 gtaccatgac gctgcaggca acgattcagg atggcgtgat ctggttatcg gatgataaga
    56461 ataatctgga agtgaactta accgcctggc aacaaccttc atgattgtga tttcccgaca
    56521 tgttgctatt cccgatggtg agcttgagat caccgccatt cgtgcgcagg gcgcgggcgg
    56581 gcagcatgtt aataagacct caacggctat tcatctgcgt tttgacattc gggcgtccag
    56641 cctgccagag tattacaaag agcgtctgct cgccgccagc catcatttga tcagcagtga
    56701 tggcgtgatt gtcattaagg cacaggaata ccgcagtcag gaactgaacc gcgaagcagc
    56761 tctggcccgg ctggtggctg tgattaaaga attaacaaca gaacaaaaag cccgacgacc
    56821 cacgcggccc acccgtgcat cgaaagagcg caggctggca tcgaaagcac aaaaatcaag
    56881 cgtgaaggcg atgcgcggca aagtgcgcag cggtcgggaa taaaaagaag gaatggatgg
    56941 tgaaaaaagc gatagtgaca gcgatggctg taatcagcct ctttactctg atgggttgta
    57001 ataatcgggc cgaagtcgat acgctttctc cggcgcaggt tgccgaactg aaaccgatgc
    57061 cgcaaagttg gcgcggcgtg ctgccgtgtg ccgattgcga aggaatcgaa acctctctgt
    57121 tcctcgaaaa agacggaaca tgggtgatga atgagcgtta tctcggtgct cgtgaagaac
    57181 cttcctcctt cgcttcctac ggtacatggg cgcgaaccgc tgacaagctg gtattaaccg
    57241 acagcaaagg tgaaaagtca tattatcggg cgaaaggcga tgcgctggag atgctcgatc
    57301 gtgaaggcaa tccgattgaa tcgcagttca actatacgct ggaaccggca caatccagtt
    57361 tacccatgac gccgatgacc ctgcggggca tgtattttta tatggctgat gcggcgacct
    57421 tcactgattg cgcgaccgga aaacgtttca tggtagcgaa taacgcagag ctggagcgtg
    57481 gctacctggc tgcgcgcggc cacagtgaaa aaccggtgtt actgtcagta gaaggtcact
    57541 ttacgcttga ggctaatccg gataccggtg cgccaaccaa agtattagca cccgatacgg
    57601 cagggaaatt ttacccaaac caggattgca gtagtttggg gcagtaaccc gtcttgagac
    57661 agaaacaaac gcaaaacagg ccagaaggat atatttcaac attttgaatt tgcacgtttt
    57721 ttgtaggccg gataaggcgt ttacgccgca tccggcaatg gtgctcaacg cctgatgcga
    57781 cgctggcgcg tcttatcatg cctacaaccc ccctcatacc ttaaggcgga taaggcattt
    57841 actttatcac cgggtcaaac caacaaagcg acccgccttg atataaatcc ctggcttcag
    57901 gtgcccaaca tactgtaact gcgtttctgg cttaaacgca gagacatcac caatacgcat
    57961 atgcatcaga tcggcagggc taatccagcc cgattgcgcc agcgtaagcg gatgcccggc
    58021 tttggcaaat gcatcagtga caaattccga acaaaaccac gactttttgt ctccttcgcc
    58081 cacactgctt aactgcgctt tcgccaggcc gctgacgcac tgttggcgaa aatctgcgga
    58141 aaaaggattc agtgagcata tctggcgggt caccataaat ggaatgaact cgacaatgcc
    58201 gcgatagtta tacccgctat ctttaatttt gttggcgaac gcggtgattt ctgtggcttg
    58261 ttgcggggta agatccggga ctcgtaagac gaaaagctta tcgctatgct tcatcgcctt
    58321 tttaagggaa acaatctgga cgccagcgcc tgtcgcttct gcaacgttat tttcacccag
    58381 atagattgca acgtgactca cagaggaagt gctgaagacg cggattccaa atgaggttac
    58441 cccaaggctt gaggagaaca gcaaatcgcc gggtttgaga tcaggtaccg ttatttcttt
    58501 tattgcttgt tcggtgaaag agctttgatg ctggaatttg acagcccatg tttttgcctc
    58561 gacatctacc gctgtggcag aggagtctgg ctggctgata tcaacggtac aggccgaaag
    58621 taaaagaaag caggggagaa gcaggcggca gtacgccttt ggtttatcca ttttatacaa
    58681 tccatgtaaa aaagggccct gaaattcagg accctttctg gcatcagcct ttaatctgtt
    58741 tcaccagata ttcgacgatg tcaccagtct taattaactg tttctcgccg ttgcgacgat
    58801 atttatattc gatatcgtcg ttgtcgaggt tacggtcgcc cagcacaata gtgtgcggaa
    58861 taccgatcag ttccatatca gcaaacatca cgcccggacg ctctttgcgg tcatccagca
    58921 gcacttcgat accttgcgcg cgcagttcgc tgtacagttt ctcagcaagc tcttgtacgc
    58981 ggaaggattt gtgcatgttc atcggcagaa tcgccacctg gaacggcgcg atagcgtcag
    59041 gccatacgat gccgcgttcg tcgtagttct gctcaatcgc cgcagctacc acacgcgtta
    59101 ccccgatacc gtagcaaccc atcgtcagga tttggttacg gccatcttca ccctgtacgg
    59161 aggctttcag tgcttcggag tacttggtac ccagctggaa gatgtgacca acttcgatac
    59221 cacgtttgat cagcagcgta ccctggccat ccgggcttgg atcgccagcc accacgttac
    59281 ggatatccgc aacttccggg gtagcgacat cgcgatccca gttgatgccg aagtagtgtt
    59341 taccatcgat gttagcacca gcagcgaaat cactcatcgc cgcaacggta cggtcaatca
    59401 ccaccggaat cggcatgttt accggaccca gtgaacccgg accggcgtta accacggcac
    59461 gaatttcttc ttcggtcgcg aaagtcagcg ggcttgcaac ctgcggcagt ttttctgctt
    59521 taacttcgtt cagttcgtgg tcaccgcgca ccagcagtgc aaccagcggg aagctactgc
    59581 cttcaaccgc tttaaccagc agagtcttaa ccgttttctc aatcggcaga ttgaactgtt
    59641 caaccagttc cgcgatggtt ttcgcgttcg gcgtatcaac cagcgtcatt tcctgggtag
    59701 cagcagcgcg cggttctttc ggcgcgatag cttctgccag ttcaatgttc gctgcatagt
    59761 cagaggtgtc ggagaagacc acatcgtctt caccgctctg cgccagcacc tggaattcgt
    59821 gagaggcgct gccgccgata gaaccggtgt cggcctgtac ggcgcggaaa tccagcccca
    59881 tgcggctgaa gattttgctg taggccgcat acattgcatc gtaggtttcc tgcagggatt
    59941 cctgagaagt atggaaagag taagcatctt tcatcaggaa ttcgcgggaa cgcatgacgc
    60001 cgaaacgcgg acgcacttca tcgcggaact tggtctggat ctgatagaag ttcagcggca
    60061 gctgtttgta agagctaagc tcgttacgaa tcaggtcagt gataacttct tcatgagttg
    60121 ggccgagtac gaacggacgc tcgccacggt caacaaaacg cagcagttcc ggaccgtact
    60181 gttcccaacg accactctct tgccacaaat cggctggctg aaccaccggc atcgacacct
    60241 cgatcgcacc ggcgttgttc atctcttcac gcacgatgtt ttcgactttt ttcagaacgc
    60301 gcacgccggt cggcagccag gtatataacc cggaggccag cttgcggatc atcccggcgc
    60361 gcagcatcag ctgatggctg atcacctcgg cgtcggcagg tgtctccttg agagtggaga
    60421 gcaggtattg gctagtacgc atgttgttac ggttccagtt ggaaggtaga acaggctcaa
    60481 ggcgagcctg ggacaaaaaa agtgatttag tttaccagtg caaaagaaat gtcaaaagag
    60541 aagggcgtga atttaacgcg gttccagcgc aaagacttca aaacctgcgt cggtgacgcg
    60601 ccagcgaacg ttaaaatcat gtagccagac ggcataggtt ttgcccgttt cctcaccttt
    60661 acgataggcc gggcgcgggt cctgcgccag tacttcgcgg ataaacagcg ttaactgcgg
    60721 ataacgcttc tccagcgtca aaagctgctt ttcgacctct gcggtaaaac tcaccgtcat
    60781 ctctgccgct ggcgcacttt gcgcatagct ggcactggca tcgggaagcg attcggcaaa
    60841 ggggagatac ggtttgatat ccaccaccgg cgtaccatcg accagatcca gactgccgag
    60901 cttcagaatc acgctgtctt tatggcaaac aacctctttc agctctacca gcgacatacc
    60961 aattgggtta gggcgaaaag tggaacgcgt agcgaaaaca cccattctgg cattaccgcc
    61021 aagacgaggc ggacgcacag tcggacgcca gccgccttcc atcgtttgat gaaagacgaa
    61081 aagtatccat aaatggctga atgcttccag gccgcgaacg gcgtcggcct ggttgtaggg
    61141 agcaatgaga tgcagttcgc cgttggcgct ttttaccaga cctggctggc gcggaacggc
    61201 gaatttttct ttatagggcg agcgaataac gcctatttgc tcgaactgga aactgctcat
    61261 ttcgccgtaa tgttaagcgc agaaccgata catacagcct gacgatagca gcctggcgta
    61321 ccgctggtga cttcgcagct atgcagtaat accgcattgg ctttcatttt cgaggcgttg
    61381 atttgcatcc gcttacgcgc ggttggaatg ctcggcggag agtcctgatt agaggcctgg
    61441 caagagtcgc cactgacttc accgagatcg cggaacggtt tgccgactaa ttcttctgca
    61501 ttggtataaa ttcggaccgg cgtggcgcgc ggcgctttcg gttttgcagg ctccgctttc
    61561 ggctggggtg cagtgctttg aacgggttcg acaggggatc tgcttaacat ggaacagccg
    61621 cttagcatga gtgctactaa acagatcggt aaagcacgca tagtatttcc tcaatgtatg
    61681 atcaaaacgt caatattgaa tcaggagctt gtaaaaatga caagacgggc aagcgcccgt
    61741 cctgaatgat attacaaatt gtggaaacag cctaaaaatt accagccttt aacagcgccg
    61801 ccgttaaaca ctttgtttgc tgcttcgtaa acttcgtcag actgataagc ctggacgaat
    61861 ttcttcacgt tctcggcgtc tttgttatct tcacgcgtta cgatcaggtt tacgtacggg
    61921 gagtctttat cttcgacaaa gataccgtct ttcgctggag tcaggccaat ctggctggca
    61981 taggtggtat tgataactgc cagagcgatt tgcgcatcgt ccagagaacg cggcagttgc
    62041 ggtgcttcca gttcaacaat tttcagattt tttgggttct caacaacatc aagaacggtc
    62101 ggcagcaggc caacgccatc tttcagtttg atcaagccca ctttttgcag cagcagcagt
    62161 gaacgaccaa ggttagttgg gtcgtttggc acggcaacct gcgaaccatc ctgcagttca
    62221 tccagtgatt tgattttctt ggagtaacca gcaatcggat aaacaaaagt gttgcctact
    62281 gcgaccagtt tgtagccacg atctttcagt tgctgatcaa ggtacggttt atgctggaag
    62341 gcgttggcgt cgatatcgcc tttgctcaat gcttcgtttg gcagaacata gtcgttgaag
    62401 gttaccagct caacgtccag gccatatttg tctttcgcaa ctttctgcgc gacttctgca
    62461 acctgctgtt cggcaccaac aatcacgccg actttaatgt ggtttggatc tttttcatcc
    62521 tgaccgcagc ctaccagtgc cagtgatcca atcagggctc ccactgccgc aaaggttttg
    62581 aatttgaacg ccatacctta ttccttcttc aattatttat gttgtgttga acgttacttg
    62641 cgagtgacag cccggacgat gcggtcgcct gcgaactgaa ttaaataaac cagaatgacc
    62701 agcaatacca gtaccgtatt catcaccgtc gcgttgtagc cgatgtagcc atactgatag
    62761 ccaatctgac ctaaaccacc ggcaccgact gcaccaccca tcgcggaata accgaccagg
    62821 gtaatcaggg tgatagttgc cgcattcacc agacccggca acgcttccgg taacagcact
    62881 ttacggacga tctgcattgg cgtggcaccc attgcgcggg aagcttcaat taacccggtt
    62941 gggatctcca gcagagcgtt ctcgaccata cgggcaataa acggtgctgc accaacggtt
    63001 aacggaacaa ttgctgcctg caaaccaatc gatgtaccga caataacgcg ggtaaacgga
    63061 atcatccata ccagcaagat aatgaacggg atggaacgga aaatgttcac aatcgcagaa
    63121 acggtacgat acagcttcgc gttagcaata atttgccccg gacgcgtgac ataaagcagg
    63181 acgccaaccg gcagaccaat cacaaaccca aaaaatccgg atacgaaggt cattgccagc
    63241 gtttcccata cgccacgaac cagcagccac atcatcggct cagacataac ccagtacctc
    63301 tacttttaca tggtgttcct gcagccaggc aatggcggct tgcgtatctt gttgtgtgcc
    63361 gtgcatttca gtcagcatga tgccgaattt aacgccacca gcgtagtcca tctgcgcgct
    63421 aataatgttg ttgttgacgt tgaaacgacg cgcggtttca gaaagcagtg gggcatcgac
    63481 cgattgaccg gtaaactcca gacgcagcat cggcacgcag tcagtgaatg gctccgcttg
    63541 cagacgttcc tggtaatctt ccgggatatc cagatgcagg gttgactgaa taaacttctg
    63601 cgccagcggc gttttcggat gtgagaacac ttcacttacc gtgtcctgct cgattagttc
    63661 gccattgctg atgaccgcaa cgcaatcaca aatacgcttc acaacgtcca tttcgtgggt
    63721 gatcaacaga atcgtcaaac ccagacggcg gttgatgtct ttcagcagtt cgagaataga
    63781 acgtgtcgtt gccgggtcca gcgcgctggt ggcttcatca cacagcaata ctttgggatt
    63841 gctggctaat gcacgggcaa tcgccacacg ttgtttctgc ccaccggaaa gattcgacgg
    63901 gtagctatca tgcttatcgc caagaccaac caatgacagc aattccgtca cgcgacgttt
    63961 gatctcgtct ttcggtgtat tgtccagctc cagcggcaga gccacgttgc caaaaacagt
    64021 acgcgaagag agcaggttaa aatgctggaa aatcatacca atctggcgac gagctttggt
    64081 caactcggat tctgacagcg tggtcagttc ctggccatcg accagcacgc taccctcggt
    64141 tgggcgctcc agcaggttta cacaacgtat aagcgtactc ttacccgcgc ctgaggcacc
    64201 gataacgcca taaatttgtc cagctggcac atgcaggctg acgttgttca acgcctggat
    64261 gatgcgggtg ccctggtgga acactttggt gatattcgaa agttttatca ttgattattt
    64321 attatcgtca ttaagttagt cgtggcatct cgaatgcctg aaacgggcaa cgccgtcaat
    64381 gaaatggatg ttaaggcatc cagacgtcta aatcaatcag gtttatgcga agagcacttt
    64441 cttgcaggtc gaaacatgcg atactagcgt cacatgcctt attaaggagc tataaaaggt
    64501 ggcgaagagc gtacccgcaa tttttcttga ccgtgatggc accattaatg tcgatcacgg
    64561 ctatgtccat gagatcgaca actttgaatt tatcaacggt gttattgacg ccatgcgcga
    64621 gctaaaaaaa atgggctttg cgctggtggt agtaaccaac cagtctggca ttgctcgcgg
    64681 taaatttacc gaagcacagt ttgaaacgct gaccgagtgg atggactggt cgctggcgga
    64741 ccgagatgtc gatctggatg gtatctatta ttgcccgcat catccgcagg gtagtgttga
    64801 agagtttcgc caggtctgcg attgccgcaa accacatccg gggatgcttt tgtcagcacg
    64861 cgattatttg catattgata tggccgcttc ttatatggtg ggcgataaat tagaagatat
    64921 gcaggcagcg gttgcggcga acgtgggaac aaaagtgctg gtgcgtacgg gtaaacctat
    64981 tacacctgaa gcagaaaacg cggcagattg ggtgttaaat agcctggcag acctgccgca
    65041 agcgataaaa aagcagcaaa aaccggcgca atgattaaaa gatgagcggt tgaaataaaa
    65101 atgcattttt ccgcttgtct tcctgagccg actccctata atgcgcctcc atcgacacgg
    65161 cggatgtgaa tcacttcaca caaacagccg gttcggttga agagaaaaat cctgaaattc
    65221 agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt g
//
LOCUS       DAZKFU020000002        58540 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZKFU020000002 DAZKFU020000000
VERSION     DAZKFU020000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 58540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.002"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..171
                     /gene="yhfL"
                     /locus_tag="ACHOQQ_RS00305"
                     /old_locus_tag="pgaptmp_000061"
     CDS             4..171
                     /gene="yhfL"
                     /locus_tag="ACHOQQ_RS00305"
                     /old_locus_tag="pgaptmp_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HGU4468379.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            467..1804
                     /gene="frlA"
                     /locus_tag="ACHOQQ_RS00310"
                     /old_locus_tag="pgaptmp_000062"
     CDS             467..1804
                     /gene="frlA"
                     /locus_tag="ACHOQQ_RS00310"
                     /old_locus_tag="pgaptmp_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGU4468380.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAVTAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1825..2847
                     /gene="frlB"
                     /locus_tag="ACHOQQ_RS00315"
                     /old_locus_tag="pgaptmp_000063"
     CDS             1825..2847
                     /gene="frlB"
                     /locus_tag="ACHOQQ_RS00315"
                     /old_locus_tag="pgaptmp_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGU4468381.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGEQASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2897..3724
                     /gene="frlC"
                     /locus_tag="ACHOQQ_RS00320"
                     /old_locus_tag="pgaptmp_000064"
     CDS             2897..3724
                     /gene="frlC"
                     /locus_tag="ACHOQQ_RS00320"
                     /old_locus_tag="pgaptmp_000064"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGU4468382.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            3724..4509
                     /gene="frlD"
                     /locus_tag="ACHOQQ_RS00325"
                     /old_locus_tag="pgaptmp_000065"
     CDS             3724..4509
                     /gene="frlD"
                     /locus_tag="ACHOQQ_RS00325"
                     /old_locus_tag="pgaptmp_000065"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HGU4468383.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIKPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPELVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4609..5340
                     /gene="frlR"
                     /locus_tag="ACHOQQ_RS00330"
                     /old_locus_tag="pgaptmp_000066"
                     /gene_synonym="yhfR"
     CDS             4609..5340
                     /gene="frlR"
                     /locus_tag="ACHOQQ_RS00330"
                     /old_locus_tag="pgaptmp_000066"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGU4468384.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNQRH"
     gene            complement(5500..6585)
                     /gene="yhfS"
                     /locus_tag="ACHOQQ_RS00335"
                     /old_locus_tag="pgaptmp_000067"
                     /pseudo
     CDS             complement(5500..6585)
                     /gene="yhfS"
                     /locus_tag="ACHOQQ_RS00335"
                     /old_locus_tag="pgaptmp_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(6597..7901)
                     /gene="yhfT"
                     /locus_tag="ACHOQQ_RS00340"
                     /old_locus_tag="pgaptmp_000068"
     CDS             complement(6597..7901)
                     /gene="yhfT"
                     /locus_tag="ACHOQQ_RS00340"
                     /old_locus_tag="pgaptmp_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HGU4468385.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDACGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWFGLFIPA"
     gene            complement(7913..8266)
                     /gene="yhfU"
                     /locus_tag="ACHOQQ_RS00345"
                     /old_locus_tag="pgaptmp_000069"
     CDS             complement(7913..8266)
                     /gene="yhfU"
                     /locus_tag="ACHOQQ_RS00345"
                     /old_locus_tag="pgaptmp_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HGU4468386.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8277..9155)
                     /gene="php"
                     /locus_tag="ACHOQQ_RS00350"
                     /old_locus_tag="pgaptmp_000070"
     CDS             complement(8277..9155)
                     /gene="php"
                     /locus_tag="ACHOQQ_RS00350"
                     /old_locus_tag="pgaptmp_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HGU4468387.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYSFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9152..10378)
                     /gene="yhfW"
                     /locus_tag="ACHOQQ_RS00355"
                     /old_locus_tag="pgaptmp_000071"
     CDS             complement(9152..10378)
                     /gene="yhfW"
                     /locus_tag="ACHOQQ_RS00355"
                     /old_locus_tag="pgaptmp_000071"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4468388.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFTTHQTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLALLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVTTQIGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10379..11542)
                     /gene="yhfX"
                     /locus_tag="ACHOQQ_RS00360"
                     /old_locus_tag="pgaptmp_000072"
     CDS             complement(10379..11542)
                     /gene="yhfX"
                     /locus_tag="ACHOQQ_RS00360"
                     /old_locus_tag="pgaptmp_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HGU4468389.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNPLEA"
     gene            complement(11626..11988)
                     /gene="yhfY"
                     /locus_tag="ACHOQQ_RS00365"
                     /old_locus_tag="pgaptmp_000073"
     CDS             complement(11626..11988)
                     /gene="yhfY"
                     /locus_tag="ACHOQQ_RS00365"
                     /old_locus_tag="pgaptmp_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGU4468390.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSTHWPAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(12005..12910)
                     /gene="yhfZ"
                     /locus_tag="ACHOQQ_RS00370"
                     /old_locus_tag="pgaptmp_000074"
     CDS             complement(12005..12910)
                     /gene="yhfZ"
                     /locus_tag="ACHOQQ_RS00370"
                     /old_locus_tag="pgaptmp_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HGU4468391.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTTLGLEATPLTDDPRFLQATEAVVLTRVDDYPIQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13173..14177)
                     /gene="trpS"
                     /locus_tag="ACHOQQ_RS00375"
                     /old_locus_tag="pgaptmp_000075"
     CDS             complement(13173..14177)
                     /gene="trpS"
                     /locus_tag="ACHOQQ_RS00375"
                     /old_locus_tag="pgaptmp_000075"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGU4468392.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14170..14928)
                     /gene="gph"
                     /locus_tag="ACHOQQ_RS00380"
                     /old_locus_tag="pgaptmp_000076"
     CDS             complement(14170..14928)
                     /gene="gph"
                     /locus_tag="ACHOQQ_RS00380"
                     /old_locus_tag="pgaptmp_000076"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGU4468393.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14921..15598)
                     /gene="rpe"
                     /locus_tag="ACHOQQ_RS00385"
                     /old_locus_tag="pgaptmp_000077"
     CDS             complement(14921..15598)
                     /gene="rpe"
                     /locus_tag="ACHOQQ_RS00385"
                     /old_locus_tag="pgaptmp_000077"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGU4468394.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15616..16452)
                     /gene="dam"
                     /locus_tag="ACHOQQ_RS00390"
                     /old_locus_tag="pgaptmp_000078"
     CDS             complement(15616..16452)
                     /gene="dam"
                     /locus_tag="ACHOQQ_RS00390"
                     /old_locus_tag="pgaptmp_000078"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4468395.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16559..17845)
                     /gene="damX"
                     /locus_tag="ACHOQQ_RS00395"
                     /old_locus_tag="pgaptmp_000079"
     CDS             complement(16559..17845)
                     /gene="damX"
                     /locus_tag="ACHOQQ_RS00395"
                     /old_locus_tag="pgaptmp_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGU4468396.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17937..19025)
                     /gene="aroB"
                     /locus_tag="ACHOQQ_RS00400"
                     /old_locus_tag="pgaptmp_000080"
     CDS             complement(17937..19025)
                     /gene="aroB"
                     /locus_tag="ACHOQQ_RS00400"
                     /old_locus_tag="pgaptmp_000080"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGU4468397.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19082..19603)
                     /gene="aroK"
                     /locus_tag="ACHOQQ_RS00405"
                     /old_locus_tag="pgaptmp_000081"
     CDS             complement(19082..19603)
                     /gene="aroK"
                     /locus_tag="ACHOQQ_RS00405"
                     /old_locus_tag="pgaptmp_000081"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGU4468398.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(20004..21242)
                     /gene="hofQ"
                     /locus_tag="ACHOQQ_RS00410"
                     /old_locus_tag="pgaptmp_000082"
     CDS             complement(20004..21242)
                     /gene="hofQ"
                     /locus_tag="ACHOQQ_RS00410"
                     /old_locus_tag="pgaptmp_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGU4468399.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSTKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21154..21558)
                     /gene="hofP"
                     /locus_tag="ACHOQQ_RS00415"
                     /old_locus_tag="pgaptmp_000083"
     CDS             complement(21154..21558)
                     /gene="hofP"
                     /locus_tag="ACHOQQ_RS00415"
                     /old_locus_tag="pgaptmp_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGU4468400.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPRWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21548..21988)
                     /gene="hofO"
                     /locus_tag="ACHOQQ_RS00420"
                     /old_locus_tag="pgaptmp_000084"
     CDS             complement(21548..21988)
                     /gene="hofO"
                     /locus_tag="ACHOQQ_RS00420"
                     /old_locus_tag="pgaptmp_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGU4468401.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRVSHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21972..22511)
                     /gene="hofN"
                     /locus_tag="ACHOQQ_RS00425"
                     /old_locus_tag="pgaptmp_000085"
     CDS             complement(21972..22511)
                     /gene="hofN"
                     /locus_tag="ACHOQQ_RS00425"
                     /old_locus_tag="pgaptmp_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGU4468402.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVWLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22511..23290)
                     /gene="hofM"
                     /locus_tag="ACHOQQ_RS00430"
                     /old_locus_tag="pgaptmp_000086"
     CDS             complement(22511..23290)
                     /gene="hofM"
                     /locus_tag="ACHOQQ_RS00430"
                     /old_locus_tag="pgaptmp_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGU4468403.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23410..25962
                     /gene="mrcA"
                     /locus_tag="ACHOQQ_RS00435"
                     /old_locus_tag="pgaptmp_000087"
     CDS             23410..25962
                     /gene="mrcA"
                     /locus_tag="ACHOQQ_RS00435"
                     /old_locus_tag="pgaptmp_000087"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGU4468404.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYYGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESVWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26123..26683)
                     /gene="nudE"
                     /locus_tag="ACHOQQ_RS00440"
                     /old_locus_tag="pgaptmp_000088"
     CDS             complement(26123..26683)
                     /gene="nudE"
                     /locus_tag="ACHOQQ_RS00440"
                     /old_locus_tag="pgaptmp_000088"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGU4468405.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            27003..29138
                     /gene="igaA"
                     /locus_tag="ACHOQQ_RS00445"
                     /old_locus_tag="pgaptmp_000089"
     CDS             27003..29138
                     /gene="igaA"
                     /locus_tag="ACHOQQ_RS00445"
                     /old_locus_tag="pgaptmp_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGU4468406.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLIDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            29203..29871
                     /gene="yrfG"
                     /locus_tag="ACHOQQ_RS00450"
                     /old_locus_tag="pgaptmp_000090"
     CDS             29203..29871
                     /gene="yrfG"
                     /locus_tag="ACHOQQ_RS00450"
                     /old_locus_tag="pgaptmp_000090"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGU4468407.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEFMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29882..30283
                     /gene="hslR"
                     /locus_tag="ACHOQQ_RS00455"
                     /old_locus_tag="pgaptmp_000091"
     CDS             29882..30283
                     /gene="hslR"
                     /locus_tag="ACHOQQ_RS00455"
                     /old_locus_tag="pgaptmp_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGU4468408.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30308..31186
                     /gene="hslO"
                     /locus_tag="ACHOQQ_RS00460"
                     /old_locus_tag="pgaptmp_000092"
     CDS             30308..31186
                     /gene="hslO"
                     /locus_tag="ACHOQQ_RS00460"
                     /old_locus_tag="pgaptmp_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGU4468409.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31249..32973)
                     /gene="yhgE"
                     /locus_tag="ACHOQQ_RS00465"
                     /old_locus_tag="pgaptmp_000093"
     CDS             complement(31249..32973)
                     /gene="yhgE"
                     /locus_tag="ACHOQQ_RS00465"
                     /old_locus_tag="pgaptmp_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGU4468410.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYCYFYQSVWHNVLILQVIFLANGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            33352..34974
                     /gene="pckA"
                     /locus_tag="ACHOQQ_RS00470"
                     /old_locus_tag="pgaptmp_000094"
     CDS             33352..34974
                     /gene="pckA"
                     /locus_tag="ACHOQQ_RS00470"
                     /old_locus_tag="pgaptmp_000094"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGU4468411.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35050..36402)
                     /gene="envZ"
                     /locus_tag="ACHOQQ_RS00475"
                     /old_locus_tag="pgaptmp_000095"
     CDS             complement(35050..36402)
                     /gene="envZ"
                     /locus_tag="ACHOQQ_RS00475"
                     /old_locus_tag="pgaptmp_000095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGU4468412.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36399..37118)
                     /gene="ompR"
                     /locus_tag="ACHOQQ_RS00480"
                     /old_locus_tag="pgaptmp_000096"
     CDS             complement(36399..37118)
                     /gene="ompR"
                     /locus_tag="ACHOQQ_RS00480"
                     /old_locus_tag="pgaptmp_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGU4468413.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37346..37822
                     /gene="greB"
                     /locus_tag="ACHOQQ_RS00485"
                     /old_locus_tag="pgaptmp_000097"
     CDS             37346..37822
                     /gene="greB"
                     /locus_tag="ACHOQQ_RS00485"
                     /old_locus_tag="pgaptmp_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGU4468414.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37919..40240
                     /gene="yhgF"
                     /locus_tag="ACHOQQ_RS00490"
                     /old_locus_tag="pgaptmp_000098"
     CDS             37919..40240
                     /gene="yhgF"
                     /locus_tag="ACHOQQ_RS00490"
                     /old_locus_tag="pgaptmp_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGU4468415.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40678..40905
                     /gene="feoA"
                     /locus_tag="ACHOQQ_RS00495"
                     /old_locus_tag="pgaptmp_000099"
     CDS             40678..40905
                     /gene="feoA"
                     /locus_tag="ACHOQQ_RS00495"
                     /old_locus_tag="pgaptmp_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGU4468416.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40922..43243
                     /gene="feoB"
                     /locus_tag="ACHOQQ_RS00500"
                     /old_locus_tag="pgaptmp_000100"
     CDS             40922..43243
                     /gene="feoB"
                     /locus_tag="ACHOQQ_RS00500"
                     /old_locus_tag="pgaptmp_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGU4468417.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43243..43479
                     /gene="feoC"
                     /locus_tag="ACHOQQ_RS00505"
                     /old_locus_tag="pgaptmp_000101"
     CDS             43243..43479
                     /gene="feoC"
                     /locus_tag="ACHOQQ_RS00505"
                     /old_locus_tag="pgaptmp_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGU4468418.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISKTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43682..44560
                     /gene="rpnA"
                     /locus_tag="ACHOQQ_RS00510"
                     /old_locus_tag="pgaptmp_000102"
     CDS             43682..44560
                     /gene="rpnA"
                     /locus_tag="ACHOQQ_RS00510"
                     /old_locus_tag="pgaptmp_000102"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGU4468419.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(44587..45357)
                     /gene="bioH"
                     /locus_tag="ACHOQQ_RS00515"
                     /old_locus_tag="pgaptmp_000103"
     CDS             complement(44587..45357)
                     /gene="bioH"
                     /locus_tag="ACHOQQ_RS00515"
                     /old_locus_tag="pgaptmp_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGU4468420.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSAREEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45395..46078
                     /gene="gntX"
                     /locus_tag="ACHOQQ_RS00520"
                     /old_locus_tag="pgaptmp_000104"
     CDS             45395..46078
                     /gene="gntX"
                     /locus_tag="ACHOQQ_RS00520"
                     /old_locus_tag="pgaptmp_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGU4468421.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46137..46712
                     /gene="nfuA"
                     /locus_tag="ACHOQQ_RS00525"
                     /old_locus_tag="pgaptmp_000105"
     CDS             46137..46712
                     /gene="nfuA"
                     /locus_tag="ACHOQQ_RS00525"
                     /old_locus_tag="pgaptmp_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGU4468422.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47073..48389
                     /gene="gntT"
                     /locus_tag="ACHOQQ_RS00530"
                     /old_locus_tag="pgaptmp_000106"
     CDS             47073..48389
                     /gene="gntT"
                     /locus_tag="ACHOQQ_RS00530"
                     /old_locus_tag="pgaptmp_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4468423.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48434..50518)
                     /gene="malQ"
                     /locus_tag="ACHOQQ_RS00535"
                     /old_locus_tag="pgaptmp_000107"
     CDS             complement(48434..50518)
                     /gene="malQ"
                     /locus_tag="ACHOQQ_RS00535"
                     /old_locus_tag="pgaptmp_000107"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGU4468424.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQEDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50528..52921)
                     /gene="malP"
                     /locus_tag="ACHOQQ_RS00540"
                     /old_locus_tag="pgaptmp_000108"
     CDS             complement(50528..52921)
                     /gene="malP"
                     /locus_tag="ACHOQQ_RS00540"
                     /old_locus_tag="pgaptmp_000108"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGU4468425.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53533..56238
                     /gene="malT"
                     /locus_tag="ACHOQQ_RS00545"
                     /old_locus_tag="pgaptmp_000109"
     CDS             53533..56238
                     /gene="malT"
                     /locus_tag="ACHOQQ_RS00545"
                     /old_locus_tag="pgaptmp_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGU4468426.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(56281..57297)
                     /gene="rtcA"
                     /locus_tag="ACHOQQ_RS00550"
                     /old_locus_tag="pgaptmp_000110"
     CDS             complement(56281..57297)
                     /gene="rtcA"
                     /locus_tag="ACHOQQ_RS00550"
                     /old_locus_tag="pgaptmp_000110"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGU4468427.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFKTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFPL
                     HEQYIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(57301..58527)
                     /gene="rtcB"
                     /locus_tag="ACHOQQ_RS00555"
                     /old_locus_tag="pgaptmp_000111"
     CDS             complement(57301..58527)
                     /gene="rtcB"
                     /locus_tag="ACHOQQ_RS00555"
                     /old_locus_tag="pgaptmp_000111"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGU4468428.1"
                     /translation="MNYELLTTENASVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDGSDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        13896 a        16163 c        14792 g        13689 t
ORIGIN      
        1 taaatgaaca aatttattaa agttgcactg gtaggtgcgg tactggctac gttaactgca
       61 tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct gctgcacccg
      121 gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta agtctctgac
      181 agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg agggcgtatt
      241 tgtttctata tccttcttgc tccccacccg taaaaatatt ttcactttaa tttcaattgg
      301 ataaccacat tcatcagcca cagcgcgatc ctgtgcgaaa ttatgtgatc ttcctccaca
      361 ttacataaca tcatatgttg ttatattcat catgcattgt catgttacct tttaaatgac
      421 tgcaaactct cccctacaac gtgttggaca agaaaaaggt atcgctatgg gaagccagga
      481 actccaacgc aagctcggat tttgggccgt tcttgcaatc gccgtcggga caaccgtcgg
      541 ctccggtatt tttgtatctg tgggtgaagt ggcaaaagcg gcgggcacgc cgtggctgac
      601 ggtgctcgca tttgtcatcg gcgggttaat tgtgatcccg caaatgtgcg tctatgcgga
      661 actatccacc gcttatccgg aaaatggcgc agattatgtt tatctgaaaa atgccggaag
      721 ccgaccgctg gctttcctct ccggctgggc cagcttctgg gccaacgatg cgccgtcatt
      781 gtcgattatg gcgctggcga tagtcagcaa tcttggcttt ttaacgccta tcgatccgtt
      841 gctcggtaaa tttatcgccg ccggattaat tatcgccttt atgctgctgc acctgcgctc
      901 cgttgaaggc ggcgcagcat ttcagacgct aattaccatc gccaaaatta tcccgttcac
      961 tatcgtcatt ggccttggga tcttctggtt taaagcggag aattttgccg cccctaccac
     1021 cactgcgatt ggcgcaacgg gcagctttat ggcgctactg gcggggatct ccgccaccag
     1081 ttggtcgtac accggcatgg cctctatctg ttatatgacc ggcgaaatta aaaaccccgg
     1141 taaaactatg ccacgagcgc taattggttc ctgtctgctg gttctggtgc tctacaccct
     1201 gctggcgttg gtgatttccg ggctgatgcc cttcgacaaa ctcgccaact ctgaaacgcc
     1261 gatttccgac gcactgacct ggatcccggc actcggcagc accgccggga tcttcgttgc
     1321 cgtcacggcg atgatcgtca ttctcggctc gctttccagt tgcgtgatgt accagccgcg
     1381 gctggaatat gcgatggcaa aagacaatct gttcttcaaa tgcttcggcc atgtgcatcc
     1441 gaaatacaac acgccggatg tctccatcat cctgcaaggg gcgctgggga tcttcttcat
     1501 cttcgtttca gatctcacca gcctgctggg ctatttcacc ctggtaatgt gtttcaaaaa
     1561 taccctcacc ttcggctcca tcatctggtg tcgtaaacgc gacgattaca aaccgctgtg
     1621 gcgtactccg gctttcgggc tgatgaccac cctcgccatt gcatcaagcc tcattctggt
     1681 tgcctcaacc tttgtctggg caccgattcc cggccttatc tgcgccgtca tcgttattgc
     1741 cactggtctg cctgcttacg ccttctgggc gaagcgtagc cgccagctca acgctttgtc
     1801 gtaatgttat ctggagaaaa taaaatgttg gatattgata aaagcaccgt ggactttctg
     1861 gtcaccgaaa atatggttca ggaagtggaa aaagttctca gccatgacgt tccgctggtg
     1921 cacaccatcg tggaagagat ggtgaagcgc gacatcgatc gtatttattt cgttgcctgc
     1981 ggatcgccac tcaacgccgc gcaaacggcg aaacatctgg cagatcgctt ttccgatctt
     2041 caggtttacg ccatttccgg ctgggagttc tgcgataaca ccccgtatcg cctcgacgat
     2101 cgttgcgcag taattggcgt ttctgactac ggtaaaaccg aagaggtaat caaagcgctg
     2161 gagctgggcc gggcctgcgg cgcactcact gcggcgttca ccaaacgcgc ggacagcccg
     2221 attacctcgg cggcggaatt tagcattgat tatcaggccg actgtatctg ggaaattcat
     2281 ctgctgctct gctacagcgt ggtgctggag atgatcaccc gcctcgcgcc gcacgcggaa
     2341 atcggcaaga tcaaaaacga tctcaagcag ctgccaaatg cgctcggtca tctggtacgc
     2401 acctgggaag agaaaggtcg ccagctcggt gaacaggcca gtcagtggcc gatgatttac
     2461 acggttgccg caggcccgct gcgcccgctg ggttacaaag aaggcatcgt gacgctgatg
     2521 gaatttacct ggacgcacgg ctgtgtgatt gagagcggtg agttccgtca cggcccgctg
     2581 gagattgtcg aaccgggcgt tccgttcctg tttctgctcg gcaatgacga aagtcgtcac
     2641 accaccgaac gcgccattaa ctttgttaaa cagcgtactg acaacgtgat cgtcatcgat
     2701 tacgccgaaa tttcgcaagg gctgcacccg tggctggcac cgttcctgat gttcgtgcca
     2761 atggagtggc tctgctacta cctgtctatt tacaaagatc acaacccgga tgaacgccgc
     2821 tattacggtg gtctggtgga atattaatcc ctctcccggc ccggcaacgg gccggattta
     2881 cgcaaggagt tacccgatga aaacaggtat gtttacctgc ggccaccagc ggctgcctat
     2941 tgaacacgca ttccgtgatg caagcgagct gggttacgac ggcatcgaaa tttggggcgg
     3001 tcgcccgcac gcgttcgcgc cggacttaaa agcgggcggc atcaaacaga tcaaggcgct
     3061 ggcgcagacg tatcagatgc cgattatcgg ttatacgcca gaaaccaacg gctatccgta
     3121 taacatgatg ctgggcgatg aacatatgcg tcgcgaaagc ctggacatga tcaagctggc
     3181 gatggatatg gcaaaagaga tgaacgcggg ttatacgctg atttctgcgg cccacgcggg
     3241 ctatctcacg ccgcctaacg ttatctgggg gcggctggca gagaacttaa gcgaactgtg
     3301 tgagtacgcg gaaaacatcg gcatggatct gatcctcgaa ccgttaacgc cgtatgaatc
     3361 gaacgtcgtg tgtaatgcta atgatgtgct tcatgcgctg gcgctggtgc cttcgccgcg
     3421 cctgttcagc atggtcgaca tttgcgcgcc gtatgtccag gcggaaccgg tgatgagtta
     3481 tttcgacaaa ctgggcgata aattacgtca tctgcatatt gtcgacagcg acggggccag
     3541 cgacacgcat tacattcctg gagaaggcaa aatgccgctg cgggaactga tgcgcgatat
     3601 tattgatcgg ggctatgagg ggtactgcac agtggaactg gtaacgatgt atatgaacga
     3661 gcccagactt tacgcccgcc aggcgctgga acgctttcgc gcgctgctgc cggaggatga
     3721 gtgatgaaaa ccctggcgac aatcggcgat aactgcgtcg atatctaccc gcaactgaat
     3781 aaagcgtttt ctggcggtaa tgcggtgaac gtggcggtgt actgcactcg ctacggtata
     3841 aagccgggat gcattacctg ggtgggtgac gatgactacg gcacaaagct gaagcaggat
     3901 ctcgcccgca tgggcgtcga tatcagccat gtccatacga aacacggcgt aaccgcacaa
     3961 actcaggtgg aactgcacga caacgatcgc gtttttggcg actacaccga aggcgtgatg
     4021 gccgactttg ccctgagcga agaagattac gcctggctgg cgcagtatga cattgtgcac
     4081 gcggcaatct ggggacatgc ggaagacgca ttcccacagc tgcacgctgc gggcaaactt
     4141 accgctttcg acttctccga caagtgggac agcccgctct ggcagacact ggtgccgcat
     4201 ctcgattttg cctttgcctc tgcaccgcaa gaagacgaag cgctgcgtct gaagatgaaa
     4261 gcgattgttg cccgtggcgc aggaacagtg attgtcacgc tgggcgaaaa cggcagcatt
     4321 gcctgggatg gcgcgcagtt ctggcgtcag gctcctgaac tggtgacggt tatcgacacc
     4381 atgggtgccg gagattcgtt cattgccgga ttcctttgcg gctggtctgc ggggatgaca
     4441 ttaccgcagg cgatggcgca gggaacggcg tgcgcggcga aaaccattca gtaccacggt
     4501 gcctggtagg tataacgttg gcgtgagcat cttcacgcca acgtgctgtt acttgccgga
     4561 aaacgaccct ataatccgag taattcattc tttatttcag ggtcgattat gtcagctacg
     4621 gaccgctact ctcatcaact cctctacgct accgtccgcc agcggctgct ggatgatatc
     4681 gcgcaggggg tttaccaggc cgggcaacag atccctaccg aaaacgagct ttgtacacaa
     4741 tataacgtca gccgcattac cattcgcaaa gccatcagcg acttagtggc agacggcgta
     4801 ctgatccgct ggcagggcaa aggcaccttt gtacaaagcc agaaggtcga aaacgccctg
     4861 cttactgtca gtggttttac cgattttggc gtctcacaag gcaaggcgac gaaagagaaa
     4921 gtgatcgagc aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa catccctggc
     4981 aacagcgaag tgttccatct ttgccgggtg atgtatctcg ataaagagcc gctgtttatt
     5041 gatagttcct ggatcccgct gtcgcgttat cctgactttg atgagattta cgtcgaagga
     5101 agctccacct atcagttatt tcaggagcgt tttgacacgc gagtggtcag cgacaaaaag
     5161 accatcgata tctttgccgc cacccgcccg caggcaaaat ggctgaaatg cgaactgggc
     5221 gaaccgttgt ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc ggtgcacgtc
     5281 tccgaactct tctgccgcgc caaccgcatc accttaacga ttgataacca acgacattaa
     5341 ccgtaggccg gataagatgc gccagcatcg catccggcga tgccggcgca ctgaatttta
     5401 catcccgcac gttcccctca ccctaaccct ctccccaaag gggcgagggg accgttcagt
     5461 gcggaattta gcttgattgc cgaacggtgc tatccagcat taaatactgg caatactctc
     5521 acgcaaaatc cgcagcaccg tctcttcacc gctgcgattc gggttaatgc gaatcgcaca
     5581 atgttcgagt tgcggattcg cctggcggaa cgttccggaa aggcgataaa agagcggcgg
     5641 gatttcatat ttcgactctg caccgaccgg gtaaggcaac gcgccacgct tttgcgcttc
     5701 ttccagcacc ctggcggcaa tcggctgatg aaactcgaca atcaacacct tcgactgcgc
     5761 attggcaatg acggcgcttt tcacttccgg cactgcacca ccgttgagca aagccagcaa
     5821 ccgttcagat acccctgcct gcaccgcgtg catcactggc gcgaacacca gcccacgcaa
     5881 cacttccaac gcatgagcgc cctggatctg gctgccgccg gagtaaagcg tggcgcgaat
     5941 ccgactaata acatcggcat cgccaaccac tgcaccaacg ccctccggcc caaacagctt
     6001 gaagcaggag aatgtcgaga cattcgcgcc gcattcacag ccgattcgcg ccaccttcat
     6061 caccgcatag ttgtcatcgg ttaacgctgg aatgccagcc aggcgcaacg ttgccagcac
     6121 atctgccagc acatagctgt cctgcggcta ctggcgcgta tgctgcacca gcgcggcatc
     6181 aggttgttgc tcgtcgacga cctgcttcag tgccgacagg tcattgaaat cagcagtaat
     6241 aagcgtcagc cccatctgct caataataac ccgtgtcgtt gggtaaacag gcgcgtcatg
     6301 caccagaaga cgctgccccg gtttgagcaa agccgccagc gccgcacgaa tcgcgccagt
     6361 acccgccccc tgtaccagcg ccgccgctgg tgcatgaaac gcatcagcca gcacctgctc
     6421 cacccgctgg gtaatgcgcg gttgattcaa ccctggagtt aaccctaaat caccgccagt
     6481 aagaaactcg ctgccgggga aatggcggca gatggtgtcc accaacgcaa actgcttttg
     6541 ctgcgcttca ttaagcgtca ggctttgcag aggaaacgtc ttcataggtg cattccttaa
     6601 gccggaataa acaggccaaa ccagtaaaga acattcagca ggatgccggt gatcattact
     6661 gccacgaccg gtgccgccat tttctgtacc ggacggccca gggattcgtt gaggaagtaa
     6721 atggcaaccg cgatagagaa tccggtataa cccgccatct taattgctgc gaaaatcgaa
     6781 ccgaccagca gcgccacttc catcagcata ttcatggcgt tacggatgtt atccgacgca
     6841 ttacgcaccg acgggtagcg tcccagccat ttgccgatag aacgaagcag caagacttcc
     6901 gccgaaataa ccactgcgcc taatactgcc gcaaccatcg gattcggtga gagatagccc
     6961 accgcataaa caaaggtaaa gcccgcaacc gcatacacgc cagtggctaa cgcggtggtg
     7021 gcaatcaacg gcacaaaacc cagcccgcgc ataaattccg ccagtgccgc ctgattaatc
     7081 agcgtttgtg attgttccgg cgttacgcct gcggagtacg ctttctccag agtgaagatc
     7141 gacacttcac tgccggcgaa aatcttcatg ctggcaacgg cggcaatcag cgcccctacg
     7201 atggcgatat acggtaagtt tttgataatc cgcgacgtgc gctcttcaaa caccgaaagc
     7261 ccgcaggcat cgatgtcatt ttcatcacga tgacgcaggt cgtgagttat cgcgatcccc
     7321 agcagcatca ccatgccaat aaagatttcg atggattcag ggttaagatg tgggaaatag
     7381 cgcacgacta ccacacgggt catcagaaca accacggcgg cgaccagact ttgcttccag
     7441 ccaaactggt agaaaatcgc caccagcggg aacagtgcaa aagcagaaac caccggcgag
     7501 cttaattccc cgaggctacc taatacatcc accggcagcg cggtcagcag ctggtttact
     7561 ggcaacaggc aagtaaggat caacacgccc cagatagcgc caagaccaaa cgccatcagg
     7621 ctgtttatcg ccagtacgcc gaggatatcg gtaggaagaa agagtaacca tgcgtttagc
     7681 agcccggttt tcagggtaaa agagatcccc accgaggcca caaaaccaat gctcagacca
     7741 aaagcgatac tccccgcctc gcgacggttc atatagcctt caatcagttg cggcaggatc
     7801 gggcggatgc cgtcatgaaa aacagccgcc gagcgatgcg ccagaagcga tgtcataccc
     7861 gtcaggcacg ccaccacgat aatctgaata tacagatcca tagtcgtgcc ttttatttca
     7921 gatggttaat cagcatcgga atcgcgtgtt cgacgtgctc aacggaaagg ccaaacgcca
     7981 cttttccttc agcgatcatt ttggcgatat gctcgtcttt cgctttaatg cctggtttgg
     8041 caatggtgca acttttgtta tagccaatca ccgcgatggc aatcgacaat gccgcgcccg
     8101 caccggtatt acacgcgccg atgtaataat ccagttgccc ggatttcacc ttcattgccg
     8161 cttccatgtc gttgtgaatg aaaacttcaa aacagccagg agccgtcgct tcaatagttt
     8221 ttttaatctg ctcacgctgt aagcctgcaa cgccaatctt tttcatgagt ctgtccttat
     8281 tggaaaaatt gagagggatt ttcacgtaac atcacatcca catcggcctg actgaatcct
     8341 gactggcgca attgcggaat aaaggtggtt agtaaatagt catagccata accaccgttg
     8401 gcttttaaat gggagcggcg cgtaatatcc atcgacagca tgacgcggtt cagcaaccca
     8461 cggtcgcgta gcgcatgaag catcgcaata cgcttttcgt ccgggtagta actgttctta
     8521 ccaatggtgt cgaactgcac atacgcgccg agatcgatca tcttcaaaat gttgtcgagg
     8581 ttgtctttca gatcgcagtg accaacggtg acgcgcgaaa gatcaactcc gtgggcttgc
     8641 aacaaagcca gttgctccag ccccatcgtg ctgaacgacg tatgcgtgga gatcgggcgt
     8701 ccggtctggt tatgtgccag cgcggcggca ataaatacct tctcttccag cggcgtaatc
     8761 ttcccttcgc tggagccgat ctccgcgatg atcccggctt tcagttccgt gccatcgata
     8821 ccctgctcaa tttcatcgac catctcctgc gccagttcct gcacgctgcg ggtcgccaca
     8881 tgttccggga aaaacgcgtc ctggtaataa ccggtacagg ccaccacgtt gatccccgtc
     8941 tcgcgcatta catcgagcat aaattgcgca ttgcgcccca tgtaacggtt ggtcatctca
     9001 atcacattac gcacgccccg ggccatcagg tcgttcatct cctggcaaat gaacgaatac
     9061 tgatcaaggc ggcagtccac gttgttttta aagccggaga gatcaatatg cagatgctca
     9121 tgggcgaggg tgtaacccgt cggatcaaaa ctcatagggt gtctcctgca aaccggaggg
     9181 aggaaagaaa agagcgacca ttttgtggcg gtggcgcacg gaaaaattca cacacggtag
     9241 cccccacatc agaaagcgtg gtgcgcacac cgatctgcgt agtgaccagc ccttgctgat
     9301 aaaccagcac tggcaccact tcacgggtat ggtggctgtg accaatggtc ggatcgttac
     9361 cgtgatcagc catgacaacc aggcaatcat ctggctgcat cgcctcaacc aggagagcaa
     9421 gattacggtc aacaacctgc aaacgttcgg cataacgtgc gacgtcttcc gcgtgaccag
     9481 cgaggtcggt ttcctgaatg ttggtgcaaa taaacgctgt ctgatgggtg gtaaattcgt
     9541 cgagagtgat atccataatc cgctggctat ccaccagatt ttgccagctc acgccataag
     9601 gattgttgac gatatctgcc accttaccca ccagcacggt tggcacgcct gcttcataca
     9661 gtttttgtgg cacctgcact ttttcatcga cgccatagcc catatgcacg acctggaaac
     9721 cgttgtcata agcgccagaa cgcggcgcat taataccaat aaagcgccct tctttgcttt
     9781 ctgcggcatc gagaatacgc tgactgtcgg ttaacaggcc gccaaatgta atgacccgac
     9841 cgacctgtac ctggtcacgc acgatacgac caattttgat tgcgtcgtca aaagagatca
     9901 cagagagatt ggcggtaatg ttatagactt ggcctaaatc cgcctcgaga ttatcgccaa
     9961 tcgcaaccgc ctgattgacc cacagaaatt gcagttcatc gccacggcgc tccacctgcc
    10021 aaccggcgga tactaatgcc tgctcaacac ggtcaatcac atcgcgaaag ggcatccgca
    10081 gcggcggtaa cgggcgcgtg cctaaaattt cctgatgccc cataaaggta tcgccacctt
    10141 catgttgcag ctctgccacg ccccaggttg cggagtctga cggctgcata tcgcctggcg
    10201 cataacccaa tgcgttgatt agccccagct tctccagcgt tggtagctgc aaatgcggca
    10261 actggctcag gatgtgacca caggtattcg ccccggcatc ctgcggacgc accagcgtga
    10321 catctttcat tgcccctacg ccaaagctat caatcactaa caccacaaat cgcgccattt
    10381 acgcctccag agggttgccg agactgtcat aacgaccaac gattttcggt tcgccacggt
    10441 gaataccgga caccagcacc acatcgctac gggtgacaaa aatctgcgtg cggaaacaga
    10501 gcaccactgc actgcttacc ggaaactcgc ctgccagcga cagggtgtag tcgatactgc
    10561 tgtcatccac agttttgaga ttggtttcag taatcttttg attttctggc gtaaacacca
    10621 gcgcatgttg cgcatgacca cgacggtagt aaccgccgcc gtagcagtag ctgtcgccac
    10681 ggaaatgatg ggagatttca cttaaccaga gcatcgcgat acgttcaggc tgatcgccct
    10741 gctggtttgc cggaatagta ccggtcagcg catgaccggg ttcggcatga gtcgcaccgt
    10801 attccgccag caatggcagc gaagtgcagc tggtcgctga aggcgcgttc agttgctcaa
    10861 tcgcaatacc agatttcgcc agttgatccc gtgcctgtat cagcgtgtga agattcggtg
    10921 tcggcaaaac tttcccggca gcctcatccc aaagcaggca agggaaatgg gttagtccgg
    10981 ctaaatgcag ccccggcagt ttcttaattt cagcaaccac ctcgtgcagc gaatgctgta
    11041 caaaaccgct ctcctgcccc ggataaagaa aatcatcgtc gctataaact ttgagcagca
    11101 cagactggat tcgcccggcc ttcaccgccg ccgcagaaac ttcccgcgct ttgtcgagag
    11161 taaacacggt gatgacgtcg gtgccctgtt caacggcgtc agcaacctga tgacaaggga
    11221 tttgtaccag atgcccctga tgcgccaccg gcaaaccagc gcggcgcatg actcgcgcct
    11281 ctttgtaatc caccgccaca atgccgctgt agcccaacgc cagcagtttt tccgccagcc
    11341 atgggttacg accgaattgt ttggtcatca gatacagttc aatgccgtaa agccgcgccg
    11401 tctcaatcag ccgtttaccg ttttccagta cctgatccac gtcgatcacc cagctgtccg
    11461 gggcgatctt gccctgctgc cacaaactta gtgcggcgga aatcagcgcc gggttctggc
    11521 gtttcaatgc ttctacaaac atcttgttgc ctcgtttctg ttccttgaat taaatattca
    11581 tttttttgaa tatttagatt gtacgacgtg gatttgtcgg gagcttcaaa cctcttcgtt
    11641 agcagccatc cataatccat aaaggttggc cagcaaatag ccttcttcac acgggttaac
    11701 ttccagtgcg aattccttca acaacacctg atgcaattgc acaacggcag gccagtgagt
    11761 ggattgcgcc agctctgcca gcaattcgtc atccagcggc tctatttctt caccacggcg
    11821 actgcgcatc agtgcactcg ccatatgtgt catcgccatc gttccttgct cactgcgcac
    11881 cggtagatgg cactctgttt ccagtacgtt gacgacctgc atcatgcctt tgcagatgtc
    11941 cttatcaata acgcctgcat cgcaaagcag gttgagtctg gtttccatat cagatgcctt
    12001 tcgtttaata actgggttcc tgttccccac tcaccacccg ttgttgatgg gcaagcaggg
    12061 cgtgtttatc aacaactgca cgcagcagtt gttgtattgg gtaatcatcg actcgcgtca
    12121 ggaccactgc ttcggtggcc tgtaaaaatc gcggatcgtc tgtgagcggc gtcgcctcta
    12181 atcccagcgt ggtcagttcg ttttccgcca ccacgttcca gataaccgca tcaacatcgc
    12241 ctttaacaat gcgttgtaaa ctctcgtgat aagagagatc gactcgttcc acatcactac
    12301 cgccaaaaaa gacatcagtc atgattttct gatccgccga acggttatcc agccccacgc
    12361 gcttcacgtt tgcggactcg cctttacggc aaatcaactg gtgctcgcca acgtaggtgt
    12421 gcggccccaa ctccagcgcg aggcataacc ctttttgcgt gagataactt tccgccgcca
    12481 gtcgggaaac caccgccatg tcatacacgc cgttaagtaa acactccacg cgaatatccg
    12541 cgccgcgcat atgcgcatag taaaaaggaa tgccatcaaa ctgggctttc aatccgctcg
    12601 ctaaaccttc gtacaagcgg gtatagggca aaggcatcgc acataccacg ttgttgatat
    12661 ccacatgact cagcaatgct ttgttatcca tctcgaccag ataactacca ttgcgcccac
    12721 ggcgttcaat ccgtatcgct ccgcttgatt ccagcgtttt caacgcggcc tgcgtcagac
    12781 caacggatga acggcattca gtggccagtt catctatagt tttcagtcga ttaccgcact
    12841 tttcacccaa caaataacgg gccagcgtcg tgataacgac gccttctttt ttgataaacg
    12901 ttcgacgcat aatatatttt caataaaatg aatgtttata tcttcattaa aatgaagata
    12961 agtggcaacg gtgagatgtg gaaacggcga ggcgcttcac attttttcgg attattgcaa
    13021 atgaggataa aaaaaccggg tttccccggt ttcagagtga tgacgaagtg atttattcga
    13081 caatgcgagc aaagggtaat tcccactggc gcttgcctgc tcccctcacc ctctccccaa
    13141 aggggcgagg ggaccgtccg agcgttttga tattacggct tcgccacaaa accaatcgct
    13201 tcgtacaccg cttttagcgt acgggaagcg tgcgcgctgg ctttttctgc gccgtctttc
    13261 atcacctgtt gcaggaaggc ttcatcgttg cggaaacggt ggtaacgttc ctgcaattca
    13321 gtcagcatac cggaaacggc atcagccact tcgcctttca gatgaccata catcttgcct
    13381 tcgaactgtt tttccagttc cgggatgctc tggcccgtta ccgcagaaag gatatccagc
    13441 aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg ctcgtcggaa
    13501 tcagtgaccg cgcgtttgat tttcttcact accgatttcg gatcttccag caggccaata
    13561 acgttattgc ggttatcgtc agacttggac atcttcttgg tcggctccag cagcgacatt
    13621 acgcgcgcgc cggatttcgg aataaacggc tccggcacct taaagatctc gccgtacagc
    13681 gcgttgaaac gctgagcgat atcgcggctc agttccaggt gctgtttctg gtcttcaccc
    13741 accggtacca gattagtttg atacagcagg atgtccgcag ccatcagcac cggatagtca
    13801 aacagaccag cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa ctgcgtcatg
    13861 cggctcagtt cgccgaagta ggtatagcag ttcagtgccc aacctaactg tgcatgttcc
    13921 ggcacgtggg actgaacaaa aatggtgctt ttctcaggat cgataccaca agccagatac
    13981 aaggccagcg tatccagcgt cgctttacgc agcttctgtg catcctggcg cacggtgatc
    14041 gcgtgttggt caacgataca gtaaatgcaa tggtagtcat cctgcatgtt tacccactga
    14101 cgcagcgcac ccatgtagtt accaatggtc aattcacctg agggctgtgc gccactaaaa
    14161 acgatgggct tagtcatttt tcgattcctg attttcgcta tgcggaagcc ctaatgcggg
    14221 cagaaggtca tttatagact gataaattac atcaggctgg ctgagatcga tagcctcgcc
    14281 gtagttatat ccgtaggtta agccaactga tgggcaacct gccgcttttg ctgcctgaat
    14341 atcattgcgt gagtcgccaa caaacagcat ctgttgtgga gcaattccca tccgctcagc
    14401 caccagtaac agcgggtccg gatgcggttt tttgttttgc acatcatcac caccaatcac
    14461 cacgctgaag tatttagcga tatctaaggc ttcgagcagc ggcgcgacga acggcgtcgg
    14521 tttgttggtg accaggccta gcggcaggcc tttagcctgc aacgcgccca acgtatcggc
    14581 aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg ccatagtagc gatcgaacag
    14641 tttacgcaga atacgtacct gttcttctgc cggaatgtcg tcatcaacgg gcggtttacc
    14701 cattgtttta cgctgagtcg cacgttcctg acgcgcccag gtcaatgcgc gctccatcag
    14761 aacatctgcg ccgttaccaa tccaggtaat aacgcgttct tcacctgcga cgggcaactc
    14821 cagcgcatac agcgccatat ctaccgcagc agcaagacca ggagcactgt cgaccagcgt
    14881 accatcaaga tcaaaagcga cgccgcgaat atcttcaaac ttattcatga cttacctttg
    14941 ccagttcact gcgcatttca tcaatgactt ttttatagtc tggctggtcg aagattgccg
    15001 aaccggcgac gaacatatcc gcgcccgccg cagcgatttc gccaatgttg ttcaccttca
    15061 cgccaccgtc cacttccaga cgaatgtcaa agccagactc gtcgatacga cgacgtactt
    15121 cgcgcagttt atccagtgtt tgcggaatga aagactgacc gccgaaacca gggttgacgg
    15181 acatcagcag gatcacatcc agcttatcca tcacgtaatc cagatagctc agaggtgtcg
    15241 ccgggttaaa taccagaccc gctttacagc cattttcttt aatcagttgc agcgtgcggt
    15301 caacatgctc ggaggcttct ggatgaaagg taatgatgct ggcaccagcg gcagcgaaat
    15361 caggcacaat gcgatcgacg ggtttcacca tcagatgtac gtcgataggg gcggtaatgc
    15421 catagttacg caaggatttc agcaccattg gcccaatcgt cagattggga acatagtggt
    15481 tatccatgac gtcaaaatgc acgacatcag cgccagctgc cagggctttt gcggtatctt
    15541 cacccaggcg ggcaaaatca gccgacagaa ttgagggggc aatcaaatac tgtttcatcc
    15601 gcttctcctt gagaattatt ttttcgcggg tgaaacgact cctggtttgt acaaagccag
    15661 cagttcgtcc acctttttac gtgtgccgcc gttgctgctt atactgcgtc gaactttgac
    15721 gacatgcaat tttgcgcgct gataccactc acgcgttaac atcgtatcgt gattggagat
    15781 cagcacagga atatggcgat caaccagacc ttcggcgatc tccgccagat gcgcttgttg
    15841 ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag acagcggcgc
    15901 ataaggcgga tcgcaataga cgacggatgc atcatctgca cgcgccatgc tatcggcgta
    15961 agactcacaa tagaaaaagg cattctgcgc tttttcagcg aagtgataca actctgcttc
    16021 cgggaaatag ggttttttgt agcggccaaa cggcacatta aactcaccgc gcagattgta
    16081 acgacacagg ccgttgtaac cgtagcggtt caaatataaa aacagtaccg cccgacggaa
    16141 cggatcctgg cttttgttga actcttcgcg gaactgatag taaacctcgg cgcaatttgt
    16201 ttcgggaaca aacagctcgc gtgcggcctg tacgtactca tcagtacgca tcttcacaat
    16261 gttatagaga ctgatcaggt cgctattgat atcggcaagg atataacgag aaaagtcggt
    16321 gttgagaaac accgacccgg cacctacaaa aggctcaacc agacattcgc ccttgggcaa
    16381 atgccgttta atatcatcaa gcaggggata cttgccccct gcccacttca aaaaagcgcg
    16441 attcttcttc atgctgacta actaattaca ccttctccgg ctgtggagaa agctccgaca
    16501 gcatccagcg cttcaggcaa ctcccgcaac gataaaccat tgcgggagat aaccttgatt
    16561 acttcagatc ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg gcctggacat
    16621 ctgctggcaa tgtagatacc gcttttttcg cctcttcttt cgaagcgtac acgccagaaa
    16681 ccaggacata ccacggctga ccattacgcg tcgtttcata gacaacgtag tttttcagat
    16741 tctctttctt cgcccaaccg ttcaggttgt cgtagttaga ggaactgctc agctgcagag
    16801 tgtaatggct ggacggtgcc gatttcaacg aaccaacatt acctgcggtc ttccctccag
    16861 cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg tactggagcc gtagtcgccg
    16921 tctcttttgg tgctggcgta gaagtcgcag ccggtgcctt cgtgctcgcc acaggagcag
    16981 ctggttcagt acgcttcggc gtctgtgcta ccggcttcgg ttccgttttc acggttgctt
    17041 gcggtttttt cggttcaatc accgcctgct gacgagctgg acgcgtagtg gccggacgtt
    17101 cagcagtttg cgttttcgcc gtgtcacgcg atgcattgcc attgcgtact ggcgcgacag
    17161 ttgctggttc agtcggcaat gtagaattga ccgccacatt gttcaactgt tgctgatttt
    17221 gtggctgggt cagcgcattg ttcaggtcac cctgcacttc aacacgttgt tgaccatccg
    17281 ttgccaccgg ggtttgccct tgagtcggcg tagaagagat cggtggcaga gaaacatcct
    17341 gctgagtatt ttccgcagac gtggttcccg gcgctggctg cacgccattc gcctgatcgg
    17401 tcgcattgcc agcaagatca atactcttct cgccagacgc ggtttgatcg ctggaagtgg
    17461 tcgagggggc ttttagcgca gaaccgatac cgatgatcaa cagcagtaga accagaatgc
    17521 cgacgcccat catcatatac tgacgagaag cgggtttact ggctgctttt ttgcgcttac
    17581 gcggacgacg ctctacgcgc tcttcatcca cggtttcatc ttcggattcg tcaatttctt
    17641 cttcgatttc cggttcctca ttgcgctctt tttgcgcacg agtcggacgg cgatcgtcag
    17701 tgtcatcaag ttcaatatca tcaaaattga tctgcggttc gccacgttca gtacgctcag
    17761 aacgttcaga agattgacga gaacgaccag tacgacgatc gctgggatcg ggtttcagct
    17821 cgtcttctgg tttgaattca tccatttaac accccactaa aaggttaatg cttaccacgt
    17881 tgcaattaac ctgaagctaa tagaccgctt gataagcggc ctgacctttc ttgttgttac
    17941 gctgattgac aatcggcaat ggcgttaaga acaagctcgt gcgaaacgcc gctgcgaact
    18001 tcactcttac caattgccaa cggaagaatt aagcgcatct ctcccgcaag gactttcttg
    18061 tcacgcagca tatgcggtaa atacgcctgc gcggacattt cgcgcggccc attgaccggt
    18121 aacccagccc gcttgagcag ggttataata cgctgcgttt cggcagaact aaactgcccg
    18181 agacgttccg acgtccgcgc cgccatcacc atacccgcag cgaccgcttc accatgtaac
    18241 caattgccat accccatttc agcttcaatg gcatgaccaa aggtgtgtcc cagattcagt
    18301 aaagcacgta acccggtttc gcgctcgtcg gcggcgacaa cttctgcctt cagttcacaa
    18361 caacggcgaa tacagtacgc cattgccgga ccgtccagac gcaacaacgc atccagattc
    18421 tcttccagcc agttaaaaaa cgcaccgtca agaataatgc cgtatttgat gacttctgcc
    18481 agccccgacg ctaactcacg cgggggaagc gttttcagac agtcgagatc caccaccact
    18541 gaagcaggct ggtagaacgc gccaatcatg tttttaccga ggggatggtt gaccgcagtt
    18601 ttgccgccaa cggaggaatc gacctgcgac agtaacgtcg tcgggacttg aatgaaacga
    18661 acaccgcgct gataactcgc cgccgcgaag ccggtcagat cgcccactac gccgccgcca
    18721 agcgccacca gcgtagtatc gcgaccatgc ggtttttgta acaacgccgt aaagacggta
    18781 tcgagtacag ccaggctttt atactgctcg ccgtcaggga ggataacgct atcgacgtta
    18841 acacccgcct gttcaagtac gccgcggacc ttatcgagat acagaggagc cagggtttcg
    18901 ttggtgacca acatgacctg ctcgcccgat ttcagcggta agaatgaagc tggttcatta
    18961 aacaaaccag atgcgatggt aattgggtaa ctacgttccc cgagagtaac gacaatcctc
    19021 tccataacgc gacatccacc ttaattactg tacccgcaga cgagtgtata taaagccaga
    19081 attagttgct ttccagcatg tgaataatct ggtttgcaac cactttagcg ctttgatcat
    19141 cagtacgaat ggtcacgtcg gcaatctctt catacagcgg attgcgttca ttggccaacg
    19201 cttccagaac ttcacgtggc ggtgtttcaa cgtgcagcaa cgggcgtttt ttatcacgct
    19261 gcgtgcgtgc aagttgcttt tcgatggtcg tttcaagata aacgacaacg ccacgagcgg
    19321 aaagacggtt acgcgtttca cgggatttca cagagccgcc gccagtagcc agcacaatac
    19381 cctgtttctc ggtcaactca ttgatgacct tttcttcgcg atcgcggaag ccttcttcgc
    19441 cttctaaatc gaaaacccag cccacatcag ctccggttcg tttctcaatc tcttgatcgg
    19501 aatcgtaaaa ttccatattg agttgttgag ctaactggcg cccaatagtg ctttttccgg
    19561 cacccatagg cccaaccaga aagatattgc gtttctctgc cattttttcg gtactactaa
    19621 gactattcgt taatgataaa cccgcttcgc tcagagagcg ccgcaggaca tgaactgaaa
    19681 cctcataaga tattgcgaga gtcagactga aaattatctc aatactccag cgggtttggc
    19741 aactgaataa atcacccatc cctctgcata tctggtcgct gcaagcgcgc tgccttgcta
    19801 ccaccgctct ggcgataaat caccgggtaa gattagcgta aaaaagacag caaaatgccg
    19861 cctgaatgat aaatcatcat catggaacgc aggcagcgaa gagcaccaac tctcaaatcg
    19921 gtactccttg tatgctaaat acctgcgcgc gtcaaataga tgaaacacgt tcagcgtaaa
    19981 aacattaccg cttttacggc tgtttactca ctggaaacca gtcgtggcgt gataaacacc
    20041 actaactcgc gtcgttcatc ttcttttccg tcatgacgaa ataattgccc gaaccaggga
    20101 atgtcgccaa gcaacggcac gctatcctga cccgatttat ttttacgggt aaaaatgccg
    20161 cccagcgcca acgtttctcc gcttttgacc tcgacctgcg tttcgatctc ctgcttatca
    20221 atcgccagca cttcgccatc ggcctgctgt agcacctgcc ccggaacgtt ctggctgatg
    20281 tgtaatttca gccggatgcg acctttttgt aacaccgtgg gcgtgacctc catccccagg
    20341 actgcctctt taaattccac cgacgtcgcg ccactttccc cgctggaaac ttgatatgga
    20401 atttcgctcc cctgtttaat gctggcaggc tgaagatgtg aggccagcag acgcggacta
    20461 gcgataatat ccagctgctg tttttgttcg agcgcagaaa gctcaagatc cagcaaacgt
    20521 ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg ccgtcgctac ggagaggtcg
    20581 ctgccaagcg tggtgacttg cccaacgcca ccagcttgtt gcgcatcggc cagcgtccat
    20641 ttcacgccta actcacgcaa acttttttca ttaatggtga caatatgcgc cgacagctca
    20701 acctgcccga ccggcagatc catttgcgct acccactgtt caagggcgct taacgccgtt
    20761 ttgttatctc gtagcaaaag gcgattggtg cgtttatcga cggtcatact ccctttggta
    20821 ctcagtagct tctccccagc tttcgccagc tctccggcgt cggcgtattg cagagtaata
    20881 ttgcgatttt ccagcggcag atttgcctgc gcccgcgcct gctccgcttc ctgacgggcg
    20941 atattgtcat tctgccaggc aatggaatgc actgagagaa tgttgccctc ctgacgcgtt
    21001 atcagtccgg cgcttttcac tacagtttgt agtgcctgct tccagggaac atctgttaga
    21061 tgtaacgaca ccgtaccgct gacgtctggc gacaccacca ggttcaactt ctcctgttca
    21121 gccagcgcct gcaacacctg agctaccgga acatcatcca ccatcagcgt cactttttgc
    21181 ggctttgccg cctgtacgcc gggtatcagc atcaacagta gtgcggctat ccattgcttc
    21241 atttgtatct ccttgccgtt gccacaacca tcgtggcggt tcgcagtttg tcccggtacc
    21301 cagcgttagt acgtctggcg tcagctgtaa aattgtccag ccgttttcca gcacatcgtt
    21361 ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt acaccgatga tgcgctcgcc
    21421 tcgccctacc atcccctgat agcgccactg gctaagttcg ctaatccggc atagatcttc
    21481 cggcggttta aaagggtcac gcataccggt taaaaggcac aatgcaatac ctgccaacaa
    21541 ccagcgttta accctcatgc ggcgtctcca gttgtagcgt gaacaaaaga tcatcacctt
    21601 ccacgcttaa tgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt gtaaatgccg
    21661 acggcactgc ttcccacagc gttttcaacg ccaactcgcc tccctgcgcg gatggatgcc
    21721 agaaaaccag ttgtgcgccg ggcaactgaa aatccagtgg cgaaaagggc agcgtttttt
    21781 cttcgctgaa gggagtggtg tcaaccaggc gatacagtgt ggcccactgc tgatgatgac
    21841 ttacccgtag tcgaattaat gcgtcgcgct cttcatggtg tgtcgatgac agaaaaatga
    21901 gcgtaactaa catcagcaac cagactgccc agcaaagctg gcggagacgg ggggatgtgg
    21961 cgaaccacca gtcaaagaac atgttcatcg ctaaccttcc ttgttaactg atactcaaat
    22021 tgccagcgtc cctgcgcatc ctgctgcgtg gccccccgct gattgagatg aaaagatgca
    22081 tcctggcgga gtgacgtttc tagtgcgttt aacgcggtaa tgcttgttgt aagccccttg
    22141 atctccagcg ttccctgctg ccagcttatc gttgtcagcc aggcgtgctc gggtaaaagc
    22201 gccgccagtg cttccagcgc agattgccag tcgcgggtaa attgtcgctg gcgctgcctt
    22261 tgcaaacgct gctcacgaat ttgttggcgc tccagcaaac gtggtttcgt tatctgtaag
    22321 ctgcgggcga gttgttgttc cccctgaagc caaacggcgt ctacgcgagc ttcggcgctg
    22381 cttgtcagac gcagtattag cgttatcccg acggccagca gcagaggcgc aacgaacatc
    22441 agcaaccaga aacgaagaaa agcggtccgg cgttgctgtc gccagggcaa aaaattaatt
    22501 ggcgggttca tcagtacgcc ttcccaagcg ccagccccag cgcgatggca aagtctccac
    22561 ctggcggcgg taacggcggc tgacgaacag aaacggcctc ccagggatca aatccgcctt
    22621 cgccacatat cgcgacgctt tcgggatcaa cggataacgc tgccgccagc tccttcgcgc
    22681 tagtcatccc caccgccagt ttgcgccccc agcgatagcg cgtcgcccac agccactgtt
    22741 cgttatcacg ccaggccaga cattgctgat gagaaggtaa aaaaggcagg aatcgctgta
    22801 atgcactggc atccggggtg atcgcactaa catgaacacg caacctttct gccagagtaa
    22861 gcagcgttgc cagctctttg ctttgcgcgg cagtcacgtt ataggcggga ctgagtgagt
    22921 cttcgctgta atcgaagcgc agggagtccg gatccatatc cagctcgcga gccatcgtcc
    22981 cggacagcca ggccgtttgc tcccgctcac cgagggacat cgacgggcgc ggaaatgacc
    23041 gctgtaatgt gcgactggcg ggaaacgcca acataatgtg atgacgctgc ggcagttcgc
    23101 gactccacgg taacaacgtt ttagccagcc gctgcgcatc aacaatccgc ccatctttga
    23161 taatgtcgtg cgccaacggc aaccgccacc agcgttgcaa aaagcattct tttgcgcccc
    23221 gcacaatagc aaccgctacc gcttcttgct gttgtaaatg caaaccaatt tgccagatct
    23281 taaatgccat tgtgatgatc tccttatcac ccgtcactct gacgggtata tcaatgcgtc
    23341 tggcttgcct ttatactacc gcgcgtttgt ttataaactg cccaaatgaa actaaatggg
    23401 aaatttccag tgaagttcgt aaagtatttt ttgatccttg cagtctgttg cattctgctg
    23461 ggagcaggct cgatttatgg cctataccgc tacatcgagc cacaactgcc ggatgtagcg
    23521 acattaaaag atgttcgcct gcaaattccg atgcagattt acagcgccga tggcgagctg
    23581 attgctcaat acggtgagaa acgtcgtatt ccggttacgc tggatcaaat cccgccggag
    23641 atggtgaaag cctttatcgc gacggaagac agccgcttct acgagcatca cggcgttgac
    23701 ccggtgggga tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg
    23761 gcaagtacca ttacccagca gctggcgaga aacttcttcc tcagtccaga acgcacgctg
    23821 atgcgtaaga ttaaggaagt cttcctcgcg attcgcattg aacagttgct gacgaaagac
    23881 gaaatcctcg agctttatct gaacaagatt tacctcggtt accgcgccta tggtgtcggt
    23941 gctgcggcac aagtctatta cgggaaaacg gtcgaccaac tgacgctgaa cgaaatggct
    24001 gtgatagccg ggctgccgaa agcgccttcc actttcaacc cgctctactc gatggatcgt
    24061 gccgtcgcgc ggcgtaacgt cgtgctatcg cggatgctgg atgaaggata tatcacccag
    24121 caacagttcg atcagacacg cactgaggcg attaacgcta actatcacgc gccggagatt
    24181 gctttttctg cgccgtacct gagcgaaatg gtgcgccagg agatgtataa ccgttatggc
    24241 gaaagtgcct atgaagacgg ttatcgcatt tacaccacca tcacccgcaa agtgcagcag
    24301 gccgcacagc aggcggtacg taataacgtg ctggactacg acatgcgcca cggctatcgc
    24361 ggcccggcaa atgtgctgtg gaaagtgggc gagtcggtgt gggataacaa caagattacc
    24421 gatacgctga aggcgctgcc gacctatggt ccgctgctgc ctgccgcagt caccagcgcc
    24481 aatcctcagg aagcgacggc gatgctggcg gacgggtcga ccgtcgcatt gagtatggaa
    24541 ggcgttcgct gggcgcgtcc ttaccgttcg gatactcagc agggaccgac gccgcgtaaa
    24601 gtgaccgatg ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg
    24661 tggctggcac aagtgccgga agtgaactcg gcgctggtgt cgatcaatcc gcaaaacggt
    24721 gccgttatgg cgttggtcgg tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc
    24781 caggcactgc gtcaggtagg ttccaacatc aaaccgttcc tctacaccgc ggcgatggat
    24841 aaaggtctga cgctggcaag tatgttgaac gatgtgccaa tttctcgctg ggatgcaggt
    24901 gccggttctg actggcagcc gaagaactca ccaccgcagt atgccggtcc aattcgctta
    24961 cgtcaggggc tgggtcagtc gaaaaacgtg gtgatggtac gcgcaatgcg ggcgatgggc
    25021 gtcgactacg ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac
    25081 accgaatcgc tggcgctggg ttcagcgtcc ttcactccaa tgcaggtggc gcgcggctac
    25141 gcggtcatgg cgaacggcgg cttcctggtg gacccgtggt ttatcagcaa aattgaaaac
    25201 gatcagggcg gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga atgtgatatt
    25261 ccggtgattt acggtgatac gcagaaatcg aacgtgctgg aaaataacga tgttgaagat
    25321 gtcgctatct cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca
    25381 aatcaggcat tagtggcgaa gactggcgcg caggagtacg caccgcacgt catcaacact
    25441 ccgctggcat tcctgattaa gagtgctttg aacaccaata tctttggtga gccaggctgg
    25501 cagggtactg gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc
    25561 ggtaccacta acagttcgaa agatgcgtgg ttctcgggtt acggtccggg cgttgtgacc
    25621 tcggtctgga ttggctttga tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga
    25681 gcgattaaag atcagatctc aggttacgaa ggcggtgcca agagtgccca gcctgcatgg
    25741 gacgcttata tgaaagccgt tcttgaaggt gtgccggagc agccgctgac gccgccgccg
    25801 ggtattgtga cggtgaatat cgatcgcagc accgggcagt tagctaatgg tggcaacagc
    25861 cgcgaagagt atttcatcga aggtacgcag ccgacacaac aggcggtgca cgaggtggga
    25921 acgaccatta tcgataatgg cgaagcacag gaattgttct gattaaaaag gcgcttcggc
    25981 gcctttttaa gtttgctgac aaagtgcact ttgtttatac cggatacggc gtgaacactt
    26041 tacccgtcta gcaacatcta gaaaaatcaa tatattgcag aaacttcgta agcctgataa
    26101 gcatcaggca aacttcacgc atttacactc gcccctgccc tttcaaccat tcgcgcacga
    26161 ggaacagcgc gctgacatta cgcgcttcgt tgaagtcagg gtcttccagc aaatccatca
    26221 tatgcgccag cggccagcgc acctgtggta gcggctctgg ctcatcgcct tccagcgatt
    26281 ccgggtagag atcttgcgct accacgatat tcattttgct ggaaaagtaa gacggtgcca
    26341 tgctgagctt cttcaaaaaa gtcagatcgt tcgcaccaaa tccaacctct tcttttagct
    26401 cacggttagc ggcttcgaag acgctttcac ccggatcaat taatcctttc gaaaaaccta
    26461 attcgtagga ttcagttccc actgcatatt cgcggatcag gatcaggtga tcgtccacaa
    26521 tcggcacaat catcactgct tcccggttgg ttggacgcat tcgttcataa acacgccgca
    26581 cgccattgct gaactccaga tccacgctct cgacggtaaa cagtcgggaa cgggctacag
    26641 tttcaacatt cagaatggtg ggtttttgta atgatttgct catcgtggga tctatgctgt
    26701 gaaatcagcg gttattgtgc gatatcggac acgctttcgg caatgtgaat tgcatgttat
    26761 ttacatttat gtaacttaat aaataatcgt cctcaaatca aattaaaagt caataggttg
    26821 aaataactcc aggaatttgc tgatattccg ccttcagagg gtttgctatg atcagcggtt
    26881 actgtgatgt gcttaatgat gctcaagtta aactccacgc ttgccgatag ccaaccgcag
    26941 aatcatgtat tgtgtccggt gcgactgacc acgcctgaca gactaagtaa gatggggaaa
    27001 gcatgagcac cattgtgatt tttttagctg ctttgctggc ctgctcacta cttgcgggat
    27061 ggctgataaa agtgcgatcc agacggcgtc agctgccctg gaccaacgcc ttcgcggatg
    27121 cgcaaacgcg taaactcaca cctgaagaac gtagcgccgt tgaaaattat cttgagagcc
    27181 tgacgcaggt attacaggtg cctggcccaa cgggagccag cgcggcaccg atctctctgg
    27241 cgctgaatgc cgaaagcaac aacgtcatga tgctgacaca cgctatcaca cgttacggca
    27301 tctctaccga cgatccgaat aaatggcgtt actacctcga ttcggtagaa gtccacctgc
    27361 cccccttctg ggaacagtac atcaacgatg agaataccgt tgaactgatt cataccgatt
    27421 cgctgccgtt ggttatttca ctcaacggtc atacgctgca ggagtacatg caggaaacgc
    27481 gcggctatgc cttgcaacct gttccgtcca cgcaggcgtc gattcgcggg gaagagagtg
    27541 agcaaatcga gctactcaat attcgcaaag aaacgcatga agaatatgcg ctgagtcgtc
    27601 cgcgcgggct gcgtgaagcg ttgctgatcg tcgcctcctt cctgatgttc tttttctgcc
    27661 tgattacccc ggatgtattt gttccgtggc tggcaggtgg cgcgttactg ctgctgggcg
    27721 caggtctgtg ggggctattc gcccccccgg caaaatcctc cctgcgggaa attcattgtc
    27781 tgcgcggtac accccgtcgt tggggattgt ttggcgaaaa cgatcaggaa cagatcaaca
    27841 atatttcact cggtattatc gacctggtct atcccgcaca ctggcagcca tacattgctc
    27901 aggatctcgg tcaacaaacc gatatcgata tctatctcga ccgccatgta gtgcgtcagg
    27961 gacgatatct ttcgctgcat gatgaagtaa aaaactttcc gttacagcac tggctgcgca
    28021 gtacgattat cgctgcgggc tcgctgctgg tgctgtttat gctgttattc tggatcccgc
    28081 tggatatgcc gctgaaattc actctctcat ggatgaaagg cgcgcagacc attgaagcca
    28141 ccagcgtaaa acaactggct gacgctggcg tgcgggtagg cgatacattg cgtattagtg
    28201 gtacgggaat gtgtaatatt cgcacttccg gaacctggag cgcgaaaacc aattcacctt
    28261 ttttaccgtt tgactgctcg cagatcatct ggaatgacgc ccgctcatta ccattaccag
    28321 aatctgagct ggtcaacaaa gcgacggcat tgactgaagc agttaatcgc cagctgcacc
    28381 ctaaaccgga agatgaatct cgcgtcagtg cctcattacg ttcagcgatt caaaaatccg
    28441 gcatggtatt gattgatgat tttggcgaca ttgtactgaa gacagcggat ctgtgttctg
    28501 ccaaagatga ctgtgtgcga ctgaaaaatg cgctggtcaa tctcggcaac agtaaagact
    28561 gggacgcgct ggtaaaacgc gccaacgccg ggaagctcga tggcgtgaat gtgttattac
    28621 gcccggtgag tgcggaatcg ctggataacc tggtggcaac ctccaccgcg ccgtttatca
    28681 cgcatgaaac ggcgcgagcg gcacaatcac taaacagtcc ggcccccggc ggattcctga
    28741 ttgtcagcga cgaaggcagc gattttgttg atcagccctg gccttcggca tcactttacg
    28801 actacccgcc gcaagaacag tggaacgctt tccagaaact ggcacaaatg ctgatgcata
    28861 cgccgtttaa cgccgagggt atcgtcacaa aaatcttcac tgacgccaat ggtacgcagc
    28921 atataggcct tcatccgatc ccggatcgtt ccggcctgtg gcgctatctc agcaccacac
    28981 tgctgctact gacgatgctg ggtagcgcca tttataatgg cgtacaggcc tggcggcgtt
    29041 accagcgtca tcgcactcgc atgatgaaga ttcaggccta ttatgaaagc tgcctgaacc
    29101 cgcaactgat caccccttca gaaagcctta tcgaataaca cgtttgcgcg gcaggttatg
    29161 ctaccctgtc gcgcaaattg cttcactctg gagattttcc tcatgcatat caacattgcc
    29221 tggcaggacg tagataccgt tctgctggat atggacggca cgttgctcga cctcgccttc
    29281 gataactatt tctggcaaaa gctggtgcct gaaacatggg gcgcgaaaaa cggggttacg
    29341 ccacaggaag cgatggaatt tatgcgccag caatatcacg acgtacagca tacgctaaac
    29401 tggtactgtc ttgattactg gagtgagcaa ctgggtctgg atatctgtgc gatgaccacc
    29461 gagatgggac cgcgtgccgt actgcgtgaa gataccattc cgtttcttga ggcactgaaa
    29521 gccagcggta agcagcgaat tttgctcacc aatgcgcatc cgcacaacct ggcggtaaaa
    29581 cttgagcata ccggtctgga cgcacacctt gatttattac tttccaccca cacatttggt
    29641 tatccgaaag aggatcagcg gttatggcat gcggtggccg aagctacggg tctgaaagct
    29701 gaaagaacgc tgtttattga tgacagcgaa gcgattctcg atgctgccgc gcaatttggt
    29761 attcgttact gcctcggcgt gactaatcct gactcaggga ttgccgagaa acagtatcaa
    29821 cgccatccgt cactgaatga ctaccgccgc ctgatcccct cgctaatgtg aaggagaggc
    29881 catgaaagaa aaacctgctg ttgaggttcg actggataaa tggctatggg ctgcccgttt
    29941 ttataaaacc cgcgcgctgg cccgtgaaat gattgaaggc ggtaaggtgc attacaacgg
    30001 gcagcgcagc aagccgagca aaatcgtcga gctgaatgcc acgctcactc tgcgccaggg
    30061 aaatgacgaa cgcacggtga ttgtaaaggc gattactgaa cagcgtcgcc ccgccagcga
    30121 ggcagccttg ctgtatgaag agactgcgga aagtgtagag aaacgcgaaa aaatggcgct
    30181 ggcacgtaaa cttaatgcct taaccatgcc gcacccggac cgacgcccgg acaaaaaaga
    30241 gcgccgcgac ctgttacgat ttaaacacgg cgacagtgaa taactgtcac ctgcaagaga
    30301 gatgattatg ccgcaacatg accaattaca tcgctatctg tttgaaaact ttgccgtgcg
    30361 cggcgaactg gtaaccgttt cggaaaccct gcaacagatc cttgagaacc acgattatcc
    30421 gcagcccgtt aaaaacgtgc tggcagaact gctggttgcg accagcctgt taaccgctac
    30481 gctgaagttt gatggtgata tcaccgtaca gctgcagggc gacggtccga tgaatctggc
    30541 agttattaac ggtaacaata accagcaaat gcgcggtgtg gcgcgcgtgc agggcgaaat
    30601 tccagaaaat gccgacctga aaacgctggt cggcaatggt tacgtggtga tcaccattac
    30661 cccgagcgaa ggcgaacgct atcagggcgt agttggtctg gaaggtgata ccctggcggc
    30721 ctgcctggaa gattacttta tgcgttctga acagctgccg acgcgcctgt ttattcgcac
    30781 cggcgacgta gacggcaaac cggctgcagg cggtatgttg ttgcaggtaa tgcctgcgca
    30841 aaatgcccag caggacgact tcgaccacct ggcgacgcta accgaaacca tcaaaaccga
    30901 agaactgctg accttaccgg caaacgaagt gttgtggcgt ttgtatcacg aagaagaggt
    30961 gacggtttac gatccgcagg atgtggagtt caaatgcacc tgctcgcgtg aacgttgcgc
    31021 cgatgcgctg aaaacgctgc ctgatgaaga agtcgatagc atcctggcgg aagatggcga
    31081 aattgacatg cattgtgatt actgcggtaa ccactatctg ttcaatgcga tggatattgc
    31141 tgaaatccgc aacaacgcgt ctccggcaga tccgcaagtt cattaatgag tatgtccggc
    31201 agagagagct ctgccggatt ttacgactaa tacacctgat gcgctttgtc actcattcag
    31261 attcacatcc agcctttcac catccactac aagatcgcgc cacgctttag gcttcgttcc
    31321 cagttttcca tccttcgcag ctgtgagtaa tttttctttc gttatatcac gcggaagtaa
    31381 actcatcgta agctcttgcc attctttctt gtcaggatca aagccataga caatgtatct
    31441 ttcatcatca aaggcaaaga ggatccgctc tgccctgcca tcactattca gatcttgctc
    31501 gaccagtacg caggcgtctt tttcaataca ggtcatcacg ttatagcgat cctggattaa
    31561 ggccgaccag aatgtcgcat ccggtttacc agaaccaggg gcaattaaca cattttcggc
    31621 taatactttt tccgatatct gttgctgaag atgttgctct ccatctaacg ccatcagtag
    31681 atcccgcgca cgtttcgggt ctttcatata ctcggcatcg cttttcagcg actcaagcgc
    31741 cgcacgtcca tagcgaccgc tgtgttcgag catgtagata gttacctggt ctggcgtgtt
    31801 tttgccgctc tgataacgcg ccatatgact gttcacacta atgcgcatac tgtccagcac
    31861 cggcgaatta agaagcacca gtattaccaa caccaataac gaaaccgcaa ggttcacttt
    31921 gccctgaaga acaaggggat tttgcccttt acgccagacg atactgacaa aataccccag
    31981 cgaccatacc agtaacacca gcaccgccag cgcgccctgc aagcggtcga cagtccagcc
    32041 gtactgagcg acccgcagcc ataacgccca ggcggcgatg aacacataca gcggagcaac
    32101 cagcagagcg gttttaatca ggcaacgcaa cggccccgtc cagggaagtg acgctttttg
    32161 cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag gccagcgtta acagcaaccc
    32221 ggcggcggag atgtggcgag aaatcgcgct caggcccgta aacggcaggg tgatgataaa
    32281 catcagggtt agcaatgata ctaacggcag caaccccgtg gcgattagcg tgaacaactt
    32341 ttgaatagag tcgattaaac gtgactgtgt tcgcgcgagg atcaccgcca gcgcggtaac
    32401 cagacctaat gtaagataca taaaccagtc ggttgcaaaa aagagcgtat tgaaaaacgt
    32461 gatgccaaca agtttaaaca actcacccca cagtaaaagc accagccacg tcaggccatt
    32521 ggcgagaaaa ataacctgta atataagtac gttatgccat actgactggt aaaaatagca
    32581 atagcggcta ctgtcattgc ggatacgaag gctttgttgt atccacggta gcaacaacat
    32641 tgccatcagc agcagatagc agccaaaatc ccaaagcgcc ttttcagctc tccaggggtt
    32701 catgccatca gtttgccact tcaaccagcc gctcatcccc agcgtggcaa taaacaccag
    32761 cgccagccat ccccaaaggc gcttctgttt aaaagagatc acggagaaga gtaaaacgga
    32821 tgaaaacgca acggtcgcag gcacgccata aacaatccaa ctgtgatttt ttccggcaag
    32881 ccaggcgatc agcagatagc aaaccagccc ctgaagtaat ccggtagcaa tcatccccca
    32941 gcgtgttgcg ggtgaaagtt caacgttatc catgttggtt atccagaatc aaaaggtggg
    33001 ttaattatcg catccgggca gtagtatttt gcttttttca gaaaataatc aaaaaaagtt
    33061 agcgtggtga atcgatactt taccggttga atttgcatca atttcattca ggaatgcgat
    33121 tccactcaca atattcccgc catataaacc aagatttaac cttttgagaa cattttccac
    33181 acctaaaatg ctatttctgc gataatagca accgtttcgt gacaggaatc acggagtttt
    33241 ttgtcaaata tgaatttctc cagatacgta aatctatgag ccttgtcgcg gttaacaccc
    33301 ccaaaaagac tttactattc aggcaataca tattggctaa ggagcagtga aatgcgcgtt
    33361 aacaatggtt tgaccccgca agaactcgag gcttatggta tcagtgacgt acatgatatc
    33421 gtttacaacc caagctacga cctgctgtat caggaagagc tcgatccgag cctgacaggt
    33481 tatgagcgcg gggtgttaac taatctgggt gccgttgccg tcgataccgg gatcttcacc
    33541 ggtcgttcac caaaagataa gtatatcgtc cgtgacgata ccactcgcga tactttctgg
    33601 tgggcagaca aaggcaaagg taagaacgat aacaaacctc tctctccgga aacctggcag
    33661 catctgaaag gcctggtgac caggcagctt tccggcaaac gtctgttcgt tgtcgacgcc
    33721 ttctgtggtg cgaatccgga tactcgtctt tccgtccgtt tcatcaccga agtggcctgg
    33781 caggcgcatt ttgtcaaaaa catgtttatt cgcccgagcg atgaagagct ggcaggtttc
    33841 aaaccagact ttatcgttat gaacggcgcg aagtgcacta accctcagtg gaaagaacag
    33901 ggtctgaact ctgaaaactt cgtggcgttt aacctgaccg agcgcatgca gctgattggc
    33961 ggcacctggt acggcggcga aatgaagaaa gggatgttct cgatgatgaa ctacctgctg
    34021 ccgctgaaag gtatcgcttc tatgcactgc tccgccaacg ttggcgagaa aggcgatgtt
    34081 gcggtgttct tcggcctttc cggcactggt aaaaccaccc tttccaccga cccgaaacgt
    34141 cgcctgattg gcgatgacga acacggctgg gacgatgacg gtgtgtttaa cttcgaaggc
    34201 ggctgctacg caaaaactat caagctgtcg aaagaagcgg aacctgaaat ctacaacgct
    34261 atccgtcgtg atgcgttgct ggaaaacgtc accgtgcgtg aagatggcac tatcgacttt
    34321 gatgatggtt caaaaaccga gaacacccgc gtttcttatc cgatctatca catcgataac
    34381 atcgttaagc cggtttcaaa agcgggtcac gcaacgaaag ttatcttcct gaccgcagac
    34441 gcctttggtg tgctgccgcc ggtttctcgt ctgactgccg atcaaaccca gtaccacttc
    34501 ctctctggct tcaccgccaa actggccggt actgagcgtg gcatcaccga accgacgcca
    34561 accttctccg cttgcttcgg cgcggcattc ctgtcgctgc acccgactca gtacgcagaa
    34621 gtgctggtga aacgtatgca ggcggcgggc gcgcaggctt atctggttaa cactggctgg
    34681 aacggcactg gcaaacgtat ctcgattaaa gatacccgcg ccattatcga cgctatcctc
    34741 aacggttcgc tggataatgc agaaaccttc actctgccga tgtttaacct ggcgatccca
    34801 accgaactgc cgggcgtaga cacgaagatt ctcgatccgc gtaacaccta cgcttccccg
    34861 gaacagtggc aggaaaaagc cgaaaccctg gcgaaactgt ttatcgacaa ctttgataaa
    34921 tacaccgaca cccctgcggg tgccgcgctg gtagcggctg gtccgaaact gtaatgactt
    34981 gaagctggag aatatctatc cagtatctta tagaaagcaa aacgggaggc accttcgcct
    35041 cccgtttatt tacccttctt ttgtcgtgcc ctgcgcccgc gttaccggca caggcagcca
    35101 ggcgcgaatg gaaagcccgc cccgctcgct ggtgccaagc tccagcatcc cgttatggtt
    35161 atccacgata cgctgcacaa tcgccagccc taatcccgtg ccgctaatgg tgcgtgcact
    35221 gtcgccacga acaaacggct ggaaaaggtg cttacgttgt tccggcgcaa ttcccggacc
    35281 gtcatcttcc acctggaacc aggcgcgatt cggctccgtc ccgctgctga ctttgatcca
    35341 gccattgccg taacgggcgg cgttgaccac catattcgcc accgcgcgtt tgatcgacag
    35401 cgggtgcatt ttcacttcaa tgctgccggg gtaaagcgcg gtttcaattt cccgctcata
    35461 gccactttcg gcagcaataa cctcaccgag tactgcgttg agatccgcca tttccatcgg
    35521 catctcctgc ccggtgcgca ggtagtcgat aaactgctca atgatggcgt tgcactcttc
    35581 gatatcttta ttgatcgatt ctgccaggta gccatcctgc tcgctcatca tctcggtcgc
    35641 caggcgaata cgcgtcagcg gtgtacgcaa gtcgtgactt acccccgcca tcagcagcgt
    35701 gcggtcatcc gccagttgct taacaccagc cgccatatgg ttaaaggcgc gggtaacgga
    35761 acgcacctcg gacgcgccat actcacgcag cggcggcgga ataatccctt taccgacctg
    35821 caaagcagca tgttcgagat cgaccaacgg tcggttctgg atacgaataa acagccacgc
    35881 cccgcctatc gccaatagca taatcgccaa cgtatagcgg aacagcggag agaaatcgcc
    35941 ctgatgaatt tcggtcagcg gcacgcgtac ccagatattg ggcgacagcc aagttttcag
    36001 ccagacgaca ggcgaacttt tgttgacctc aacgcgcact tccgtcgggc cgcccagttg
    36061 ctgcgccatc tgatggctta agaattcata gtgttgcgcc caacgcagac ctgcctcttc
    36121 ggcagcctcg ttggagtaga gagagatccc cagctcacgg tagatctccc gacggaaagc
    36181 gggaggcaca accaactgcg tgccgtcctc cagttgcagt ttgtcggtca tcaacatacg
    36241 cacttcgtac gcgaggactt tattaaactg ctggaggctc ggcaaaatcg cgaagttcag
    36301 caccaccaga taagtcgtca ccaggctggc gaacagcaag gtgacgatga gcaataacgt
    36361 acgggcaaat gaacttcgtg gcgagaagcg caatcgcctc atgctttaga gccgtccggt
    36421 acaaagacgt agcccaagcc ccaaacggtc tgaatgtaac gcggatgcgc tggatcttct
    36481 tccaccatgc ggcgcagacg cgaaatctgc acgtcgatgg agcgttccat tgcggaatat
    36541 tcacgaccac gggcaaggtt catcagctta tcgcgggaga gcggctcacg cggatggctg
    36601 accagtgcct tcagtaccgc aaactcaccg ctggtgagcg gcatcggctc gtcttcgcgg
    36661 aacatttcgc gcgtaccgag gttaagtttg aacttaccga aagcaattac cgcctcttcc
    36721 tgtgacggtg cgcctggtag ctcgttcgcc tgacgacgca gcaccgcacg gatacgggcc
    36781 agcagttcac gcgggttaaa cggttttgga atgtaatcgt cagcgccaat ctccaggcca
    36841 acgatacggt ccacttcttc ccctttcgcc gtcaccataa tgatcggcat cgggttgctc
    36901 tgactacgaa gacgtcggca aatcgacaag ccatcttcac caggtaacat caaatccagt
    36961 accataagat ggaaagattc acgagtcagc aggcgatcca tctgttctgc attagcgacg
    37021 cttcgaacct ggaagccttg ttcggtgaga taacgctcca gcagcgcgcg caggcgcatg
    37081 tcgtcatcga ccaccagaat cttgtagttc tcttgcattg tttgtactcc caaaggttcg
    37141 caacaatttg taagcgtgta ttcttaaaaa agctcacgtt cgtcaccagc taaatctggt
    37201 atgaatttca gcctaaattg ttacaaagca tattaaacag cagcttaagt atacaattta
    37261 ttcggcgaaa cattattgat tctgttgata tgatcacgtt atacccaatg tgcgcattat
    37321 caaacagaca aagggaatca acgagatgaa aacgcccctg gttacccggg aagggtatga
    37381 aaaactcaaa caagagctta attatctctg gcgtgaagaa cgcccggagg tcacaaaaaa
    37441 ggtgacctgg gccgcaagtc tgggcgaccg cagcgaaaat gctgactatc agtataataa
    37501 aaagcgtctg cgtgaaatcg accgtcgcgt gcgctatctc actaaatgca tggaaaatct
    37561 caaaatcgtc gattactccc ctcagcagga aggcaaagtc ttttttggcg cgtgggtgga
    37621 gattgaaaac gacgatggcg tgactcaccg tttccgtatt gtcggctacg atgaaatttt
    37681 tggccgtaaa gattacatct ctatcgattc cccgatggcc cgcgcattgc tgaaaaaaga
    37741 agtcggcgat ctggcggtgg tgaatacccc tgccggggaa gcgagctggt atgttaatgc
    37801 tatcgagtac gtgaaaccgt aaggaagagt cttaacctcc tgccgatggc tggcattttt
    37861 gccagccagt ccgtataact atcccctgat ttttgatccg aaaagatgaa ctcaaaccat
    37921 gatgaatgat tcgttctgcc gcattattgc gggtgaaatt caggcgcgcc cggaacaggt
    37981 tgacgctgcc gttcgcctgc ttgacgaagg gaataccgtg ccttttattg cacgttatcg
    38041 taaggaaatc accggcggtc tggatgacac gcagctgcgt aatctggaaa cgcgtctgag
    38101 ctatctgcgc gagctggaag agagacgtca ggcgatcctc aagtccattt ccgagcaagg
    38161 caaactcacc gatgatctgg cgaaggccat caacgccacc ttaagcaaaa ccgaactcga
    38221 agacctctac ctgccctaca aacctaaacg ccgcacccgc gggcaaatcg ccattgaagc
    38281 agggcttgag ccgttggctg acctgctgtg gagcgatccg tcacacacgc cagaagtcgc
    38341 cgctgcgcaa tatattgatg cagataaagg cgtggcagat accaaagccg cgctggacgg
    38401 cgcgcgctat atcctgatgg aacggtttgc cgaagatgcc gcgctgctgg cgaaagtgcg
    38461 tgattatctg tggaagaacg cgcatttggt ttcgacggtg gtgagcggta aagaagagga
    38521 aggggcgaaa ttccgcgact atttcgatca tcacgaaccg ttgtccacgg tgccttctca
    38581 ccgcgcgctg gcgatgttcc gtgggcgtaa cgaaggcgta ctccagcttt cgctgaatgc
    38641 cgatccgcag ttcgatgagc cgccaaaaga gagctattgc gagcaaatca tcatggatca
    38701 tctcggcctg cgcctgaaca atgctccggc ggatagctgg cgcaaaggcg tggtgagctg
    38761 gacctggcgc atcaaggtgc tgatgcatct ggaaaccgaa ctgatgggca ccgtgcgcga
    38821 acgcgcggaa gatgaagcaa tcaacgtctt tgcccgtaac ctgcacgatc tgctgatggc
    38881 ggcccctgcc ggactgcgtg caacgatggg cctcgatccg ggtctgcgta ctggggtaaa
    38941 agtggcggtg gtcgatgcca ctggcaaact ggtagcgacc gataccattt acccgcacac
    39001 cggacaagcc gcaaaagcag cgatgactgt tgctgcgctg tgtgaaaagc ataacgttga
    39061 actggtggcg atcggcaacg gtacagcttc ccgcgaaacc gaacgtttct atctcgacgt
    39121 gcagaagcag ttcccgaaag tgaccgcaca gaaagtgatc gtcagcgaag ctggcgcgtc
    39181 ggtttactcg gcttccgagc tggcagcgca ggagttcccg gatctcgacg tttcgctgcg
    39241 tggcgcggtt tctatcgccc gccgtttgca ggatccgctg gcggagctgg tgaaaatcga
    39301 tccgaaatct atcggcgtag gtcagtatca gcatgacgtc agccagacgc aactggcccg
    39361 caaactggat gcagtagtag aagactgcgt aaacgccgtt ggcgtcgatc tcaacaccgc
    39421 ttctgttccg ctattaactc gcgtggcggg cctgacgcgc atgatggcgc aaaacatcgt
    39481 ggcctggcgc gatgagaacg gccagttcca gaaccgccag caactgctga aagtcagccg
    39541 tctggggccg aaagccttcg agcagtgcgc gggcttcctg cgcattaacc acggtgacaa
    39601 cccgctggac gcctctaccg ttcacccgga agcctatccg gtggtggaac gcattctggc
    39661 agcaacacag caggcactga aagatctgat gggtaacagc agcgaactgc gtaacctgaa
    39721 agcgtctgac tttactgatg aaaaattcgg tgtgccgaca gtaactgaca tcatcaaaga
    39781 gctggaaaaa ccgggtcgcg atccgcgtcc ggaatttaaa accgctcagt ttgccgatgg
    39841 cgtcgagaca atgaacgacc tgcaaccggg tatgatcctc gaaggcgctg tcaccaacgt
    39901 caccaacttt ggcgcgtttg tcgatatcgg cgtgcatcag gacggcctgg ttcacatctc
    39961 ttcattgtcg aacaagtttg tggaagatcc gcataccgtg gtgaaagcgg gcgacattgt
    40021 gaaggtgaaa gtgctggaag tggatcttca gcgtaaacgt atcgccctga ctatgcgtct
    40081 ggacgagcag cctggcgaaa ccaactctcg tcgcggcggc ggtaacgatc gcccgcaaaa
    40141 caaccgcccg gcagccaaac cacgcggtcg tgaagcgcag cctgccggta acagcgcgat
    40201 gatggacgcg ctggcggcag caatgggcaa aaaacgttaa acgcccggac tggcccctaa
    40261 acctgcgcga taaggcgttc acgccgcatc tggcaacgaa cgccttgcct gacataaaag
    40321 tgccggagaa tatctccggc atatttattc cacagccaaa ctcacaatat atttcgttaa
    40381 tatttatcac ttcattaaca actgaaacct taattaaaca ttagccagtc ctggtaattc
    40441 gctattcgaa ttatattttc gctgcgatat aaccttgagc cacatcaaca ttgagtcaga
    40501 ttattattca aaccaacatt cgcacacatt ttaagtattg ctgatagaaa ccattctcat
    40561 tatcattgta ctgttgatta tttaatctct ccttcgttgg caaatcatct ggtctcatgt
    40621 cgctgtcaaa cgccccatga ggtagttatc cagttaatga gaaacaagta ggcacctatg
    40681 caatacactc cagatactgc gtggaaaatc actggctttt cccgtgaaat cagcccggca
    40741 tatcgccaaa aactgctttc tcttggcatg ttacctggct cctcttttaa tgtggtgcgc
    40801 gtcgctccac tcggcgaccc cattcatatc gaaacccgtc gtgtgagcct ggtattacgc
    40861 aaaaaagatc tggccttatt agaagtggaa gcggtttcct gttaatacgg tgataacaac
    40921 aatgaaaaaa ttaaccatag gcttaattgg taatccaaat tctggcaaga caaccttatt
    40981 taaccagctc actggcgcac gtcagcgtgt aggtaactgg gctggcgtta ccgtcgaacg
    41041 taaagaaggg caattctcca ccaccgatca tcaggtcacg ctggtggacc tgcccggcac
    41101 ctattctctg accaccatct catcgcagac ctcgctcgat gagcaaatcg cctgtcacta
    41161 cattttgagt ggcgacgccg acctgctgat taacgtggtg gatgcgtcta accttgagcg
    41221 taacctgtac ctgacgctac aactgctgga actcggcatt ccctgcatcg tggcactgaa
    41281 catgctcgac attgccgaga agcaaaatat tcgtattgaa attgatgctc tgtcggcgcg
    41341 tcttggctgc ccagtgatcc cgctggtttc aacccgtggt cgcggtattg aagcgctcaa
    41401 gctggcgatt gatcgctata aagctaacga gaatgtggaa ttggtgcatt acgcacagcc
    41461 gctgctcaac gaagcagatt cactggcaaa agtgatgcct tccgacatcc cgctgaaaca
    41521 acgtcgctgg ctgggcctgc aaatgctgga aggcgatatc tacagccgcg cctacgccgg
    41581 tgaagcgtcg cagcatctgg atgccgccct cgcccgtctg cgtaatgaga tggacgatcc
    41641 ggcgctgcac attgccgatg cgcgttacca gtgcattgct gccatctgtg atgtggtaag
    41701 caacaccctg acggcagaac ccagccgttt caccactgcg gtagataaaa tcgtgctcaa
    41761 ccgtttcctc ggtctgccga ttttcctctt tgtgatgtac ctgatgttcc tgctggctat
    41821 caacatcggc ggggcgttac agccgctgtt tgacgtcggc tccgtggcgc tatttgtgca
    41881 tggtattcaa tggattggct acacgctcca cttcccggac tggctgacta tcttcctcgc
    41941 ccagggcctg ggtggcggta ttaacaccgt gctgccactg gtgccgcaga tcggcatgat
    42001 gtacctgttc ctctccttcc ttgaggactc cggctatatg gcgcgtgcgg cgtttgtgat
    42061 ggaccgcctg atgcaggcgc tgggcttgcc ggggaaatcc tttgtgccgc tgatcgtcgg
    42121 tttcggttgt aacgtaccgt cggtaatggg tgcacgtaca cttgatgcac cgcgtgaacg
    42181 tctgatgacc atcatgatgg caccgtttat gtcctgcggc gcgcgtctgg ctatcttcgc
    42241 agtattcgcg gctgccttct tcgggcagaa cggtgcgctg gcggtcttct cgctgtatat
    42301 gctcggtatc gtgatggcgg tgctgactgg cctgatgctc aagtacacca tcatgcgcgg
    42361 tgaagcgacg ccgtttgtca tggagctgcc ggtctaccat gtaccacacg ttaaaagcct
    42421 gattatccag acctggcagc gcctgaaagg cttcgttctg cgtgctggta aagtgattat
    42481 catcgtcagc attttcctga gcgctttcaa cagcttctcg ttgagcggga aaatcgtcga
    42541 taacatcaac gactcggcgc tggcttccgt cagccgggtg atcaccccgg tcttcaagcc
    42601 gattggcgtg catgaagata actggcaggc aacagttggc ctgtttacgg gtgcaatggc
    42661 gaaagaagtg gtggtgggta cgctcaacac cctctacacc gcagaaaata ttcaggacga
    42721 agagttcaat ccggcggaat ttaacctcgg tgaagagttg ttcagcgcgg tagatgaaac
    42781 ctggcagagc ctgaaagaca ccttcagcct tagcgtactg atgaacccca ttgaagctag
    42841 caaaggcgac ggcgaaatgg gtaccggggc gatgggtgtg atggatcaga aattcggtag
    42901 cgcagctgcc gcatacagct acctgatttt cgtcctgctg tatgtaccgt gcatctcggt
    42961 gatgggagcc atcgcccgtg aatcaagccg tggctggatg ggcttctcca tcctgtgggg
    43021 gctgaatatc gcttactcgc tggcaacatt gttctatcag gtcgccagct acagccagca
    43081 tccaacttac agcctggtgt gcattctggc ggttatcctg tttaacatcg tggttatcgg
    43141 tctgctgcgc cgcgcgcgta gccgggtgga tatcgaactg ctggcaaccc gcaagtcggt
    43201 aagtagttgc tgcgcagcca gcaccaccgg tgattgccat taatggcttc acttattcag
    43261 gtgcgtgatt tgttggcgtt acggggccgt atggaagcgg cccagataag caagacattg
    43321 aacactccac agccaatgat taacgccatg ctgcaacaac tggaaagtat gggcaaagcc
    43381 gtgcggattc aggaagaacc tgacggctgc ctctccggca gttgtaaaag ctgcccggaa
    43441 ggaaaagcct gtctgcgcga gtggtgggcg ctgcgttaac catactccat cgcctgtttt
    43501 gaaaagcccg gtatgcgttt gcatccgggc ttttttgcgt gcggctttcc ataaaaatgc
    43561 aattcttgca gcgcgacgta agttcctctg aaagcgtctc gcagggatga aaactggcta
    43621 atgcacaggt gtggagtggc gcgtagagtc gcggcattca aacaacaggt gaaggaacgc
    43681 catgagcaaa aagcagagtt ccaccccaca cgatgcgctg ttcaaactat ttttacgcca
    43741 accggagacg gcacgcgatt ttcttgcgtt tcatttaccg gcacccattc acgcgctctg
    43801 tgatatgaaa accctcaagc tggagtcgag cagctttatt gatgacgatc tgcgtgaaag
    43861 ctattccgac gtgctgtggt cggtgaaaac ggaacaagga ccaggataca tctattgtct
    43921 gattgaacat caaagcacct caaacaaact gatcgcattc cgcatgatgc gttacgccat
    43981 tgccgcaatg caaaatcacc ttgatgccgg atacaaaacg ttgccgatgg tggtgccatt
    44041 gttgttttat cacggtattg aaagccccta tccctattcg ctgtgttggc tggattgttt
    44101 cgccgatccc aacctggcaa ggcagctcta tgcctctgca tttccgctga ttgatatcac
    44161 cgtcatgcct gatgatgaaa tcatgcagca ccgacgcatg gcgctgctgg agttaattca
    44221 aaaacatatt cgtcaacgcg acctgatggg gctggtagaa caaatggcct gcttattaag
    44281 tagtggatac gctaatgaca gacaaatcaa agggctgttt aattacatac tgcaaaccgg
    44341 cgatgcggta cgttttaacg attttatcga cggcgttgcc gaacgttcac cgaaacacaa
    44401 ggagagttta atgactattg cggaaagatt gcggcaggag ggggaacaat ccaaagcctt
    44461 gcatatagcc aaaataatgc ttgaatccgg agtccctctt gcagacatca tgcgctttac
    44521 cgggctgtca gaagaagagt tggctgcggc gagtcagtaa agttctgtct cgccattcaa
    44581 aagtgcctac accctctgct tcaacgccac cagcaggtga caaaactcga ccggatgcga
    44641 aataaacggc gcatgggccg ctttggcgaa gatatacgac tcgctgtgcg gccagagttt
    44701 atccagcatc ggcaccactt tgcgcggcac cagaccgtcg agataaccat acagtcgcaa
    44761 aaacggcatg ggtacgtttt gcagcggctg gcggagatcg accgttttca ggatttccag
    44821 cccaccattg agcacgtcaa cctccggcat cggtaacgcc agaacggttt tcttcaacgc
    44881 ccgcgcatcc tgacgcgccg tttcagtacc catggtttgt aacgcaagga accgttccac
    44941 cgtacgctga aaatcatcac tgagttgctg ctgaaatccc gccagcacgt ccggttttat
    45001 ccccggccac tcttcacgag cgctgaaaca cggcgacgac gccacggtga ccagcgcctg
    45061 aacacgctcg ggatgggtta acgcaatctg gcttgccacc agcccgccca gactccagcc
    45121 taaccaaatg gctttatcag gtgcctgtcg cagcacggct tcggccatat cagcaagtga
    45181 catcgcacca aatccccggc tacgcccgaa gccgggcagg tcaacaagat gcagcgtaaa
    45241 atgcgagcta agttcctcgt caatgcaacg ccacacttcg gcattcagtc cccatccgtg
    45301 cagcagcaca agatgaacat tcccctgacc tttggtctgc caccagatgt tattcatccg
    45361 ctattgttct cttttgactt acaaggatga acatatgcta acagtaccgg gattatgctg
    45421 gctatgccga atgccgctgg ctttaggtca ttgggggatt tgttcggtct gctcacgcgc
    45481 cgcccgtaca gataaaacgt tatgcccaca atgtggatta cccgccacac actcccatct
    45541 tccctgcggt cgctgcctgc aaaaaccgcc gccctggcaa agactggtca cggttgccga
    45601 ctatgcgccg ccgttaagtc tgctcatcca ccagcttaag ttttcccgcc gcagcgaaat
    45661 cgccagcgcc ctgtcacgtc tgttactatt ggaagtctta cacgctcgtc gcaccaccgg
    45721 gttgcaattg ccggatcgta tcatcagcgt tccgttatgg cagcggcgtc actggcgtcg
    45781 gggatttaat cagagcgatt tgctgtgtca gccgttatca cgctggttgc actgccaatg
    45841 ggatagcgaa gccatcacac gtacacgggc cactgcgacc cagcattttc ttagcgcccg
    45901 gctacgcagg cgcaacctga aaaatgcctt tcgccttgaa ttgcctgtgc aaggtcgcca
    45961 tatggtgatt gtggatgatg tcgttaccac cggaagtacc gtcgcagaga ttgcgcagtt
    46021 gcttttacgc aatggtgcgg cgactgtcca ggtctggtgc ctttgtcgaa ccttgtagag
    46081 cctcgatgat gggcgtatta taaccaacta aaatagtcaa ctattaggcc attactatga
    46141 tccgtatttc cgatgctgca caagcgcact ttgccaaact gctggcaaat caggaagaag
    46201 ggacacaaat ccgcgtattt gtgattaacc ctggcacgcc taacgctgaa tgtggtgttt
    46261 cttattgtcc gccggatgcc gtggaagcca ccgacaccgc cctgaaattt gacctgctga
    46321 ccgcgtatgt tgatgagtta agcgcaccat acctggaaga tgcagagatc gattttgtta
    46381 ccgaccagtt gggttctcag ctaacactga aagcgcctaa cgccaaaatg cgtaaagtgg
    46441 cagacgatgc accactgatg gagcgcgtgg agtatatgct gcagtcgcag atcaacccac
    46501 agcttgctgg tcacggtggc cgcgtttcac tgatggaaat caccgaagac ggttacgcca
    46561 ttctgcaatt tggcggcggc tgtaacggtt gttccatggt cgatgtgacg ctgaaagaag
    46621 ggatcgagaa gcagctacta aacgaattcc cggagctgaa aggtgtgcgc gatctcaccg
    46681 aacaccagcg cggcgaacac tcctactact aagttatccc ctcttttgta gattgcccga
    46741 tgcgacgcta aagcgtcatt tcgggcctac gttgaacatg tgccagacgt tggcgcagca
    46801 ttgctttggc aaatccctcc cttcgtttcc ccgcgataat atgaccaacc tctcataatt
    46861 taaatttacc ccgctctggt gattctcaaa cgccagatgt tacccgtatc attcacatgg
    46921 gtaccaaaca tactcctgac atctgactac aataattagt tttagtgggt atcagtcgtg
    46981 gtgccgcaat atctctgttc ccgattggga taattagagt ttgtcgtcag aaaattgacg
    47041 ttacccataa caaatgaaag gccaggtaaa tcatgccatt agtcattgtt gctatcggtg
    47101 taatcttgtt gttgctcctg atgatccgct tcaaaatgaa cggcttcatc gctctcgtcc
    47161 tcgtggcgct tgctgttgga ttaatgcaag gaatgccgct ggataaagtt attggctcca
    47221 tcaaagccgg tgtcggcggg acgctcggta gccttgccct gatcatgggt tttggcgcaa
    47281 tgctgggcaa aatgctggca gactgcggtg gcgcacaacg tatcgccacc acgctgattg
    47341 ccaaatttgg taaaaaacac atccagtggg cggtggtact gaccggtttt accgttggtt
    47401 ttgccctgtt ctatgaagtg ggctttgtgc tgatgctgcc gctggtgttt accatcgcgg
    47461 cttctgcgaa tattccactg ctgtatgttg gtgtaccaat ggcggctgca ctgtctgtga
    47521 cccacggctt cctgccaccg catccgggtc cgactgcgat tgccaccatt ttcaatgccg
    47581 atatgggtaa aaccctgctg tacggtacta ttctggcaat cccgaccgtg attctcgccg
    47641 gtccggttta cgctcgcgtg ttgaaaggta tcgataagcc aattccggaa ggtctctaca
    47701 gcgcgaaaac cttcagcgaa gaagagatgc cgagctttgg cgtcagcgtc tggacctctc
    47761 tggtgccggt agtactgatg gcgatgcgtg cgattgccga aatgatcctg ccgaaaggtc
    47821 acgctttcct gccggtagcg gagttcctcg gtgacccggt aatggctacg ctgattgccg
    47881 tgctgattgc gatgttcacc tttggtctga accgtggtcg ttcaatggat cagattaacg
    47941 acacgctggt ttcttccatc aaaatcattg cgatgatgct gttgatcatc ggtggtggcg
    48001 gtgcgttcaa gcaggtgctg gtagacagcg gcgtggacaa atacattgct tccatgatgc
    48061 acgaaaccaa catttctccg ctgctgatgg cctggtcgat tgctgccgta ctgcgtatcg
    48121 cgctgggttc cgcaaccgtt gcggcaatca ctgcgggtgg tatcgcggca ccgctgattg
    48181 caacgacggg tgttagcccg gagctgatgg ttattgcggt tggttccggt agtgtgattt
    48241 tctctcatgt gaacgatccg ggcttctggc tgttcaaaga gtactttaac ctgactatcg
    48301 gcgagaccat caaatcctgg tcgatgctgg aaaccattat ctcggtgtgc ggtctggtag
    48361 gctgtctgct gctgaatatg gtgatttgat aacactgact gccggatgca tcgcatccgg
    48421 catcaaccgc actctacttc ttcttcgctg cagctctgcg ccgtctgtcc aaatccttca
    48481 gcaacttgtt cacgccatca tcggcaaaca tcgtctcaag cgttgcggaa agcttgcgtc
    48541 gccagttttt atactggtaa ctggtgccag gaatattcac cggttcggcc atatccagcc
    48601 agtcttccgg ctgtagtcct aacagagcac tgttactgtc ggcaatgtag cgctgcaaac
    48661 cacggttcag cgtcggcgtc atcgacatca acgatgcctt atgcccggca cgttttggca
    48721 gacaaccata tttatgcagt gcatccagca gcccttgctt cgccagttcg cgatcctgat
    48781 acagaccgcg cagtaccact tcatccggat acagccccag ggttttgccc agagttagat
    48841 ccccgctctc ccagtaacca cgcagcgttg gcaggtcgtg tgtcgccgca accgccatcg
    48901 actgctccgg gtacgctttc ggtgcacgga acgtcttctc gtggtcgttt tcgaaataga
    48961 gcactttgta ggagtacaca ccgctgctgc gcagcttacc gacaatctct accggtacgg
    49021 taccgagatc ttcaccaatc accatacagc gatgacgttt actttcgagt gccagaatcg
    49081 agagcagatc atccaccgga tagtgaacat acgcgccctg atctgccgtc tcgccatacg
    49141 gtatccacca caaacgcagc atcgacataa catggtcaat tcgtaatgcg ccgcagtttt
    49201 gcatattggc acgcaacagc tcgataaacg gttcataggc acgcgcggtg atgatatgcg
    49261 ggtccattgg cggcaatccc cagttctgcc ccaacgggcc gaggatatcc ggcggcgcgc
    49321 caaccgacgc tttcaggcaa tacagttcac ggtcgcacca ggtttccgcc ccaccttccg
    49381 ctacgccaac cgccagatca cgatacaagc caatcggcat ttcatagccc tggcttatct
    49441 cccagcaggc ggcaaactgg ctgtaagcca gccactgcag ccagagataa aaatcaacgt
    49501 catcgcgatg ttcttcgcag aactgacgca cttctggtga atccacgttc tgatacatct
    49561 ctggccatgc aggccagccc cagcgcattt cgtcctcttt cacttgctgg gcatgtagcg
    49621 catcaaaggc tgcctgccag aacaggctgt cgccttgatc tgcaacaaac tggcgaaacg
    49681 cggccatctg ctcatcatca cgttgcgcga aacctttcca cgccattcgt aatgctgtca
    49741 tttttagggc ggtaaccgtg gagtaatcga cccagtcggc atcccgcgcc tgttgcagcg
    49801 tctgttgcgt ggtcggcaac tgccaccagg cctgagcctc ttcgctaaga tggaaatctt
    49861 cgacggcgtt aacgtcgata taaatcacat tcagccagcg gcgagaagac gggctgtatg
    49921 ggctggcgct ctccggattt gccggataga gcgcatgaat cgggttcagg ccgatgaacg
    49981 acccgccacg ttttgccaca tccaccagca tcgctttgag atcgccaaaa tccccaatac
    50041 cccagttttt ttccgatcgc agcgtataaa gctggacgca ggcaccccac agcttttgtt
    50101 tattcagcaa cgcctgcggt tcgtaacagc gtttcggggc gacaatcacc ctgcaatgcg
    50161 cgcgctggtc ttcctgggta agcgtcagcg tgtgataacc ttccggcagc ttcgtcggta
    50221 gattgaacgc tttgccccca gttacatggc ctttgtactg cgttccttct tcggtggtca
    50281 gcagccagct atattcgccg ctgccctcca ccaccatcgg catttttttg ccgctggtat
    50341 aaaccatgac attcgggact ggcgttaccg ccactttcgt ggcggtacgt tgatgcatcg
    50401 cgtcaagcaa acgccgtttg gtttcggcgc taatcgactg cggtttaccg tgggcattga
    50461 tgtaattggg gctaatcccc gccgccagcg cggcattatc cagacgtttg ctttccatcg
    50521 agcttcctta gcgttttgcc tgccagatac gagcctgata atcgcgaata gagcgatccg
    50581 agctaaacat accgcagcgg gcggtattga ggatcgccgc gcgagtccag gcctcctggt
    50641 cgcggtacag cacatccacc tgcttttgtg cctctacata ggctgcgaaa tccgccatca
    50701 ccagatacgg atcgccgccc tgtttgccga tactgtgcag catctggtcg aaggcatgct
    50761 tatcgccgtc gctgtattta ccgctttcca gctctttcaa tactgcgtcc agcaccttat
    50821 ctttcttccg ccatttcacc gggtcgtagc ctttggccag aattgccttc acttgttcca
    50881 cggtatgacc aaaaataaag atattttctt caccgacttt ctcggcgatt tcaacgttcg
    50941 ccccatccag cgtaccgaca gtaagcgcac cattgagcgc cagtttcata ttgccggtac
    51001 cggaagcttc tttacctgca gtcgaaattt gttcggagat atccgccgcc gggatcagtt
    51061 tttccgccgc cgaaacgcaa taatccggca ggaacaccac cttcaactta tcgccaacca
    51121 gcggatcgtt gttgatcacg tcagccactt tgttgatcgc aaagataata ttcttcgcca
    51181 ggtagtagcc cggtgccgct ttcgcgccga agaggaagac gcgcggtacg cgatcagcct
    51241 gcgggttttc acgaatttct ttgtacaacg ccagaatatg cagcagattc aggtgctggc
    51301 gtttgtactc atgcagacgt ttgatctgaa tatcgaaaat cgcctgtgga ttgatctcaa
    51361 taccggtacg aactttcaca aactccgcca gacggacttt attcgcctgc ttgatctcgc
    51421 gatattgctg acggaatttc gcatcatcag cgaatttttc cagattgatc agctgatcga
    51481 gatcgttagc ccactctttt ttcagtgatt tatccaacag agccgccagt gccgggttgc
    51541 actgtttgat ccagcgacgt ggggtaatac cgttggtgac gttatggaat ttgttcggcc
    51601 atagctggtg atattccggg aacagatctt tcaccaccag atccgagtgc agcgccgcaa
    51661 caccgttcac cgcgaaaccg ccaaccacac acaggttcgc catatgcact tgtttgtcgt
    51721 gcaccaccgc cagtttggcc cacacttttt catcgcccgg ccaggttttc tccaccagcg
    51781 ttttaaagcg agtattaatt tcgttaataa tctgcatgtg gcgcggcagt aagcctttca
    51841 ccagtttcac atcccagcgt tccagcgctt ctggcatcag ggtatggttg gtgtaagcga
    51901 aagttttgct ggtaatggcc caggcgtcat cccagctcat ctggtgctca tcgatcagca
    51961 cgcgcagcag ttctggaatc gcgatagttg ggtgggtatc gttcagctga ataacttcgt
    52021 aatccgccag ttcgtgcagt ttacgccccg ccagatgatg gcgacgcaaa atatccgcta
    52081 ccgaacaggc acactggaag tactgctgca tcaagcgcag ctttttaccg gcagtatggt
    52141 tgtcgtttgg atagagaact ttggtcagtt tttccgcatt gatgccctgc tgttcggcac
    52201 gcaggaaatc accgtcgtta aatttagtca gatcaaacgg atgcgcgtgc gtcgcctgcc
    52261 acagacgcag cggctgcgcc acgccattac gatagccgac aacggggaga tcccacgctt
    52321 gaccggtaat ggtaaactcc ggctcccagc gtccgtcttt cgtcacttta ccgccaatcc
    52381 ctacctgcac atccagtgct tcgttgtggc ggaaccacgg gtagttactg cgatgccagt
    52441 catccggcgc ttcaacctgt ttgccatcga caaaagactg gcggaacaaa ccatattgat
    52501 agttcagacc gtaacccgtc gcagactgac cgacagttgc cattgaatcg aggaagcacg
    52561 ccgccagacg tcccagacca ccgttaccca gcgccgggtc gatctcttct tccagcaggt
    52621 cagtcagatt gatgtcataa gccttcaacg aatcctgtac atcctgatac cagccgagat
    52681 tcaacaggtt gttgcccgtc agacgaccaa tcaaaaactc cattgagatg tagttaacat
    52741 gtcgctgatt cgccaccggc ttggcgaatg gctgagcacg cagcatttcg gccagtgctt
    52801 cactcactgc cagccaccac tggcgaggag tcatttcagc cgcagaattt aagccataac
    52861 gctgccactg acgtgaaagc gcttcctgaa attgcttatc gttaaaaata ggttgtgaca
    52921 taggagttcc acttttctta gattttcaac acaacgttat cgctagtttg ccaggctcga
    52981 tgttgacctt cctcatcctg cgggggatta ggcagggagg agttgcgggg atgagcaagg
    53041 aaatgtgatc tcaaccactt aaagctagtg caaaccacag gattagcagc aaatcaatgc
    53101 catacagcgc agaaaatctg tatctaagtg caaaaaatgg ccgttgcgta ttttcaaaaa
    53161 gcggaaggta actctataaa ttaagtaaag gagtgaaaca gtttcacaag taaaatatcc
    53221 agtgtgctcc atctcattct taatagattt attaagatca tctttttaga tagcactttc
    53281 atcaggaatg aagaagaaac ccttgcttaa atgaatctga tgaacataag ggaaaccagt
    53341 attcacgctg gatcagcgtc gttttaggtg agttgttaat aaagatttgg aattgtgaca
    53401 cagtgcaaat tcagacacat aaaaaaacgt catcgcttgc attagaaagg tttctggccg
    53461 accttataac cattaattac gaagcgcaaa aaaaataata tttcctcatt ttccacagtg
    53521 aagtgattaa ctatgctgat tccgtcaaaa ttaagtcgtc cggttcgact cgaccatacc
    53581 gtggttcgtg agcgcctgct ggctaaactt tccggcgcga acaacttccg gctggcgctg
    53641 atcacaagtc ctgcgggcta cggtaagacc acgctcattt cccagtgggc ggcaggcaaa
    53701 aacgatatcg gctggtactc gctggacgaa ggtgataacc agcaagagcg tttcgccagc
    53761 tatctcattg ccgccgtgca acaggcaacc aacggtcact gtgcgatatg tgagacgatg
    53821 gcgcaaaaac ggcaatatgc cagcctgacg tcactcttcg cccagctttt cattgagctg
    53881 gcggaatggc atagcccact ttatctggtc atcgatgact atcatctgat cactaatcca
    53941 gtgatccacg agtcaatgcg cttctttatt cgccatcaac cagaaaatct caccctggtg
    54001 gtgttgtcac gcaaccttcc gcaactgggc attgccaatc tgcgtgttcg tgatcaactg
    54061 ctggaaattg gcagtcagca actggcattt acccatcagg aagcgaagca gttttttgat
    54121 tgccgtctgt catcgccgat tgaagccgca gaaagcagtc ggatttgcga tgacgtttcc
    54181 ggttgggcga cggcactaca gctaatcgcc ctctccgccc ggcagaatac ccactcagcc
    54241 cataagtcgg cacgccgcct ggcgggaatc aatgccagcc atctttcgga ttatctggtc
    54301 gatgaggttt tggataacgt cgatctcgca acgcgccatt ttctgttgaa aagcgccatt
    54361 ttgcgctcaa tgaacgatgc actcatcacc cgtgtgaccg gcgaagaaaa cgggcaaatg
    54421 cgcctcgaag agattgagcg tcaggggctg tttttacagc ggatggatga taccggcgag
    54481 tggttctgct atcacccgct gtttggtaac ttcttgcgcc agcgctgcca gtgggaactg
    54541 gcggcggagc tgccggaaat ccaccgtgcc gccgcagaaa gctggatggc ccagggattt
    54601 cccagcgaag cgattcatca tgcgctggcg gcaggcgatg cgctgatgct gcgcgatatt
    54661 ctgcttaatc acgcctggag tctgttcaac catagcgaac tgtcgctgct ggaagagtcc
    54721 cttaaggccc tgccgtggga cagcttgctg gaaaatccgc agttggtgtt attgcaggcg
    54781 tggctgatgc aaagccaaca tcgctacggc gaagttaaca ccctgctagc ccgtgctgaa
    54841 catgaaatca aggacatcag agaaggcacc atgcacgcag aatttaacgc tctgcgcgcc
    54901 caggtggcga ttaacgatgg taatccggat gaagcggaac ggctggcaaa actggcactg
    54961 gaagagctgc cgccgggctg gttctatagc cgcattgtgg caacctcggt gctgggtgaa
    55021 gtgctgcact gcaaaggcga attgacccgc tcactggcgc taatgcagca aaccgaacag
    55081 atggcacgcc agcacgacgt ctggcactac gccttatgga gtttaatcca gcaaagtgaa
    55141 attctgtttg cccaagggtt cctgcaaacc gcgtgggaaa cgcaggaaaa agcgttccag
    55201 ctgatcaacg agcagcatct ggaacagttg ccgatgcatg agtttctggt gcgcatccga
    55261 gcacaactgt tatgggcctg ggcacggctg gatgaagccg aagcgtcggc gcgtagcggg
    55321 attgaagtct tgtcgtctta tcagccacag caacagcttc agtgcctggc aatgttgatt
    55381 caatgctcgc tggcccgtgg tgatttagat aacgcccgta gccagctgaa ccgtctggaa
    55441 aacctgctgg ggaatggcaa atatcacagc gactggatct ctaacgccaa caaagtccgg
    55501 gtgatttact ggcaaatgac cggcgataaa gccgccgctg ccaactggtt gcgtcatacg
    55561 gctaaaccgg agtttgcgaa caaccacttc ctgcaaggtc aatggcgcaa cattgcccgt
    55621 gcacaaatct tgctgggcga gtttgaacct gcggaaattg tcctcgaaga actcaatgaa
    55681 aatgcccgca gtttgcggtt gatgagcgat ctcaaccgta acctgttgct gcttaatcaa
    55741 ctgtactggc aggccggacg taaaagtgac gcccagcgtg tgttgctgga cgcattaaaa
    55801 ctggcgaatc gcaccggatt tatcagtcat tttgtcatcg aaggcgaagc gatggcgcaa
    55861 caactgcgcc agctgattca actcaatacg ctgccggaac tggaacagca tcgcgcacag
    55921 cgtattctgc gagaaatcaa tcaacatcat cggcataaat tcgcccattt cgatgagaat
    55981 ttcgttgaac gtctgctaaa tcatccagaa gtgccagaac tgatccgcac cagcccgttg
    56041 acccaacgtg aatggcaggt cctggggctg atttactccg gttacagcaa cgaacaaata
    56101 gcgggtgaac tggaagtggc ggcaaccacc atcaagacgc atatccgcaa tctgtaccaa
    56161 aaactcggcg tcgcccaccg tcaggctgcc gtgcagcacg cgcagaaact gctgaagatg
    56221 atgggttatg gagtgtgagt ttagccggat aatgcattgg atccggctta catctttata
    56281 tcattcaata ctcacccgcg ttacgccatc cgtttctatc aaaccaaacc gcaccggcaa
    56341 gaaacgctcc accaccgcga tattggtcag cagatggcat gagggatggg cgaccgtaaa
    56401 ttgtcccgcg cccgccagcg ccattggcaa cactagctgg tcagcaagat attcccccac
    56461 tgccgccggg cttgccaggt agcgtttcac ctctttcacc aactgtgccg caaccacctc
    56521 ggcactgacg cgcttttcac cgacgacaaa aaagcgttcg gtgatatttt cactttcgac
    56581 ttcaagcgag acggtattac ccggcccctg gtcgcgcggc aggttatgaa tatactgctc
    56641 atgcagggga aaactccccg ccagtgtagc gatttcacgc tcagcaacat ggcgcggcac
    56701 accagccagt agcacctctc cacgcatctg cacaatgttc ccgcgctcgc caagttgcaa
    56761 ggttttaaac gatgccactg gcgagacttc tgttgccacc acgccgcctc cggcagggta
    56821 aaaaccgtga cgtaacagcg tggtttgctg atgaattcct attttcgcca gcagcggctc
    56881 caacacccgg cggataaaat ccgcaggcgg ggccgacggg ttatcggtac cgccgctcac
    56941 ttcaacacgc gaaggtccat cggcaaacca cagcgcaggc agcaccgttt gcagcaccag
    57001 cgtacaactt cctgcgctac cgatagcaaa gcggtaatcg ccgccacgca cggtgccggg
    57061 ccggaagacc agacgctgcg accccagctc cgcgccttcc accgttgccc tacaaatttc
    57121 cgcagctgct tttaccgcgg tcagatgctg gcgcaacagt cccggttttg cccgcccggc
    57181 acgaatgccg gtgatggtaa atggctgacc ggttatcatc gacagactca gcgccgagcg
    57241 caggatctgc ccgccacctt cgccctgtgc gccatccagc gcaatcatcc ttttcatcat
    57301 ttatcctttt acgcacacca cctgacgcag ggtatagata acttccacca gatcgctttg
    57361 tgccgccatc accgcatcaa tatctttata cgccatcggg atttcgtcga tcacttcggc
    57421 atctttacgg cattccacat gcgcggtggc acgaatttga tcttccacgc tgaacatttt
    57481 tttcgcttta gtacggctca taacccgccc ggcaccgtgg ctgcacgaac agaacgactc
    57541 ttcatttccc agcccacgga cgataaagct tttcgctccc atcgaccctg gaataattcc
    57601 atattgacca gcacgcgcag acaccgcgcc tttacgcgtc acgtagatct cttcgccaaa
    57661 gtgctgttct ttttgcacat agttgtggtg acagttgatc tcttccattg ccagcgtttg
    57721 tggctgtctt accgttttct gcgtaatgct ctgcaacgcc gttaccacgt tttccatcat
    57781 cgcatcgcgg ttaaggctgg caaaaagctg cgcccaggcc accgctttca ggtaatcatc
    57841 aaagtattcc gtaccttcca taaagtacgc cagatcacgc gacggcaacg tttcaagctg
    57901 gtcctgcatc tctttttgcg ccagatcgat aaaataagtt ccgatggcgt taccaattcc
    57961 gcgtgaaccg gagtgcagca taatccacac ctgatccgac ccatcaaggc agatttcaat
    58021 aaagtggtta cccgttccca gcgttcccag gtgtttatag ttgttagtgt taaggaaacg
    58081 cggatatttt tgcgttaacc actgataacc ggcctccagc tcagcccatt tcgcatcgac
    58141 gttaacaggc gggttttccc atgcgccttt atcacgttta caacggccag tggtacgccc
    58201 gtgcggcacc gcagtttcaa tcgcctgacg cagctccgcc aggttttccg gcaggtcagc
    58261 cgccgttaaa gcggtacgta gtgcgttcat tccacaaccg atatccacgc ccaccgccgc
    58321 cggaataatc gcccctttgg tcgggatcac gctaccaatg gtggaacctt tacccaggtg
    58381 tacatcaggc attaccgcaa tatgtttgaa aataaacggc atcttcgccg tattaataag
    58441 ttgctgacgc gcatcggctt ctaccggcac gcctttggtc cacattttta ccgaggcatt
    58501 ttcagtggtc agtaattcgt aattcatttt gtttttctct
//
LOCUS       DAZKFU020000003        51460 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZKFU020000003 DAZKFU020000000
VERSION     DAZKFU020000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 51460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.003"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..722
                     /gene="elfD"
                     /locus_tag="ACHOQQ_RS00560"
                     /old_locus_tag="pgaptmp_000112"
     CDS             21..722
                     /gene="elfD"
                     /locus_tag="ACHOQQ_RS00560"
                     /old_locus_tag="pgaptmp_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4468429.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            747..3347
                     /gene="elfC"
                     /locus_tag="ACHOQQ_RS00565"
                     /old_locus_tag="pgaptmp_000113"
                     /pseudo
     CDS             747..3347
                     /gene="elfC"
                     /locus_tag="ACHOQQ_RS00565"
                     /old_locus_tag="pgaptmp_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3338..4408
                     /gene="elfG"
                     /locus_tag="ACHOQQ_RS00570"
                     /old_locus_tag="pgaptmp_000114"
     CDS             3338..4408
                     /gene="elfG"
                     /locus_tag="ACHOQQ_RS00570"
                     /old_locus_tag="pgaptmp_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4468430.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRNGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARINVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4420..4962
                     /gene="ycbU"
                     /locus_tag="ACHOQQ_RS00575"
                     /old_locus_tag="pgaptmp_000115"
     CDS             4420..4962
                     /gene="ycbU"
                     /locus_tag="ACHOQQ_RS00575"
                     /old_locus_tag="pgaptmp_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4468431.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANIVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4970..5485
                     /gene="ycbV"
                     /locus_tag="ACHOQQ_RS00580"
                     /old_locus_tag="pgaptmp_000116"
     CDS             4970..5485
                     /gene="ycbV"
                     /locus_tag="ACHOQQ_RS00580"
                     /old_locus_tag="pgaptmp_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4468432.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5451..6188
                     /gene="ycbF"
                     /locus_tag="ACHOQQ_RS00585"
                     /old_locus_tag="pgaptmp_000117"
     CDS             5451..6188
                     /gene="ycbF"
                     /locus_tag="ACHOQQ_RS00585"
                     /old_locus_tag="pgaptmp_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4468433.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSLRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6299..7309
                     /gene="pyrD"
                     /locus_tag="ACHOQQ_RS00590"
                     /old_locus_tag="pgaptmp_000118"
     CDS             6299..7309
                     /gene="pyrD"
                     /locus_tag="ACHOQQ_RS00590"
                     /old_locus_tag="pgaptmp_000118"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGU4468434.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7483..8025
                     /gene="zapC"
                     /locus_tag="ACHOQQ_RS00595"
                     /old_locus_tag="pgaptmp_000119"
     CDS             7483..8025
                     /gene="zapC"
                     /locus_tag="ACHOQQ_RS00595"
                     /old_locus_tag="pgaptmp_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGU4468435.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8022..9131)
                     /gene="ycbX"
                     /locus_tag="ACHOQQ_RS00600"
                     /old_locus_tag="pgaptmp_000120"
     CDS             complement(8022..9131)
                     /gene="ycbX"
                     /locus_tag="ACHOQQ_RS00600"
                     /old_locus_tag="pgaptmp_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGU4468436.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9375..11483
                     /gene="rlmKL"
                     /locus_tag="ACHOQQ_RS00605"
                     /old_locus_tag="pgaptmp_000121"
     CDS             9375..11483
                     /gene="rlmKL"
                     /locus_tag="ACHOQQ_RS00605"
                     /old_locus_tag="pgaptmp_000121"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGU4468437.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11495..13402
                     /gene="uup"
                     /locus_tag="ACHOQQ_RS00610"
                     /old_locus_tag="pgaptmp_000122"
     CDS             11495..13402
                     /gene="uup"
                     /locus_tag="ACHOQQ_RS00610"
                     /old_locus_tag="pgaptmp_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4468438.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13532..14785
                     /gene="pqiA"
                     /locus_tag="ACHOQQ_RS00615"
                     /old_locus_tag="pgaptmp_000123"
     CDS             13532..14785
                     /gene="pqiA"
                     /locus_tag="ACHOQQ_RS00615"
                     /old_locus_tag="pgaptmp_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGU4468439.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14790..16430
                     /gene="pqiB"
                     /locus_tag="ACHOQQ_RS00620"
                     /old_locus_tag="pgaptmp_000124"
     CDS             14790..16430
                     /gene="pqiB"
                     /locus_tag="ACHOQQ_RS00620"
                     /old_locus_tag="pgaptmp_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGU4468440.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16427..16990
                     /gene="pqiC"
                     /locus_tag="ACHOQQ_RS00625"
                     /old_locus_tag="pgaptmp_000125"
     CDS             16427..16990
                     /gene="pqiC"
                     /locus_tag="ACHOQQ_RS00625"
                     /old_locus_tag="pgaptmp_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGU4468441.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17246..17413
                     /gene="rmf"
                     /locus_tag="ACHOQQ_RS00630"
                     /old_locus_tag="pgaptmp_000126"
     CDS             17246..17413
                     /gene="rmf"
                     /locus_tag="ACHOQQ_RS00630"
                     /old_locus_tag="pgaptmp_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGU4468442.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17483..18001)
                     /gene="fabA"
                     /locus_tag="ACHOQQ_RS00635"
                     /old_locus_tag="pgaptmp_000127"
     CDS             complement(17483..18001)
                     /gene="fabA"
                     /locus_tag="ACHOQQ_RS00635"
                     /old_locus_tag="pgaptmp_000127"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGU4468443.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFNKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18070..19830)
                     /gene="ycbZ"
                     /locus_tag="ACHOQQ_RS00640"
                     /old_locus_tag="pgaptmp_000128"
     CDS             complement(18070..19830)
                     /gene="ycbZ"
                     /locus_tag="ACHOQQ_RS00640"
                     /old_locus_tag="pgaptmp_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4468444.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPILIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20016..20468
                     /gene="matP"
                     /locus_tag="ACHOQQ_RS00645"
                     /old_locus_tag="pgaptmp_000129"
     CDS             20016..20468
                     /gene="matP"
                     /locus_tag="ACHOQQ_RS00645"
                     /old_locus_tag="pgaptmp_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGU4468445.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20544..21599)
                     /gene="ompA"
                     /locus_tag="ACHOQQ_RS00650"
                     /old_locus_tag="pgaptmp_000130"
     CDS             complement(20544..21599)
                     /gene="ompA"
                     /locus_tag="ACHOQQ_RS00650"
                     /old_locus_tag="pgaptmp_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGU4468446.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IDNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKAHNNVTGESEKNHDTGVSPVFAGGVEWAITPE
                     IATRLEYQWTNNIGDAHTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTK
                     HFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQ
                     GLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRR
                     VEIEVKGIKDVVTQPQA"
     gene            complement(21956..22465)
                     /gene="sulA"
                     /locus_tag="ACHOQQ_RS00655"
                     /old_locus_tag="pgaptmp_000131"
     CDS             complement(21956..22465)
                     /gene="sulA"
                     /locus_tag="ACHOQQ_RS00655"
                     /old_locus_tag="pgaptmp_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGU4468447.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLPPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22684..23313
                     /gene="sxy"
                     /locus_tag="ACHOQQ_RS00660"
                     /old_locus_tag="pgaptmp_000132"
     CDS             22684..23313
                     /gene="sxy"
                     /locus_tag="ACHOQQ_RS00660"
                     /old_locus_tag="pgaptmp_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGU4468448.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23276..25438)
                     /gene="yccS"
                     /locus_tag="ACHOQQ_RS00665"
                     /old_locus_tag="pgaptmp_000133"
     CDS             complement(23276..25438)
                     /gene="yccS"
                     /locus_tag="ACHOQQ_RS00665"
                     /old_locus_tag="pgaptmp_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGU4468449.1"
                     /translation="MAYMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25448..25894)
                     /gene="yccF"
                     /locus_tag="ACHOQQ_RS00670"
                     /old_locus_tag="pgaptmp_000134"
     CDS             complement(25448..25894)
                     /gene="yccF"
                     /locus_tag="ACHOQQ_RS00670"
                     /old_locus_tag="pgaptmp_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGU4468450.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26017..28071
                     /gene="helD"
                     /locus_tag="ACHOQQ_RS00675"
                     /old_locus_tag="pgaptmp_000135"
     CDS             26017..28071
                     /gene="helD"
                     /locus_tag="ACHOQQ_RS00675"
                     /old_locus_tag="pgaptmp_000135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGU4468451.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28103..28561)
                     /gene="mgsA"
                     /locus_tag="ACHOQQ_RS00680"
                     /old_locus_tag="pgaptmp_000136"
     CDS             complement(28103..28561)
                     /gene="mgsA"
                     /locus_tag="ACHOQQ_RS00680"
                     /old_locus_tag="pgaptmp_000136"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGU4468452.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28657..29319)
                     /gene="yccT"
                     /locus_tag="ACHOQQ_RS00685"
                     /old_locus_tag="pgaptmp_000137"
     CDS             complement(28657..29319)
                     /gene="yccT"
                     /locus_tag="ACHOQQ_RS00685"
                     /old_locus_tag="pgaptmp_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGU4468453.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29492..29905
                     /gene="yccU"
                     /locus_tag="ACHOQQ_RS00690"
                     /old_locus_tag="pgaptmp_000138"
     CDS             29492..29905
                     /gene="yccU"
                     /locus_tag="ACHOQQ_RS00690"
                     /old_locus_tag="pgaptmp_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGU4468454.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29950..30267)
                     /gene="hspQ"
                     /locus_tag="ACHOQQ_RS00695"
                     /old_locus_tag="pgaptmp_000139"
     CDS             complement(29950..30267)
                     /gene="hspQ"
                     /locus_tag="ACHOQQ_RS00695"
                     /old_locus_tag="pgaptmp_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGU4468455.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30325..31515)
                     /gene="rlmI"
                     /locus_tag="ACHOQQ_RS00700"
                     /old_locus_tag="pgaptmp_000140"
     CDS             complement(30325..31515)
                     /gene="rlmI"
                     /locus_tag="ACHOQQ_RS00700"
                     /old_locus_tag="pgaptmp_000140"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGU4468456.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCNQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31610..31888
                     /gene="yccX"
                     /locus_tag="ACHOQQ_RS00705"
                     /old_locus_tag="pgaptmp_000141"
     CDS             31610..31888
                     /gene="yccX"
                     /locus_tag="ACHOQQ_RS00705"
                     /old_locus_tag="pgaptmp_000141"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGU4468457.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31885..32214)
                     /gene="tusE"
                     /locus_tag="ACHOQQ_RS00710"
                     /old_locus_tag="pgaptmp_000142"
     CDS             complement(31885..32214)
                     /gene="tusE"
                     /locus_tag="ACHOQQ_RS00710"
                     /old_locus_tag="pgaptmp_000142"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGU4468458.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32305..32964)
                     /gene="yccA"
                     /locus_tag="ACHOQQ_RS00715"
                     /old_locus_tag="pgaptmp_000143"
     CDS             complement(32305..32964)
                     /gene="yccA"
                     /locus_tag="ACHOQQ_RS00715"
                     /old_locus_tag="pgaptmp_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGU4468459.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33171..33258)
                     /locus_tag="ACHOQQ_RS00720"
                     /old_locus_tag="pgaptmp_000144"
     tRNA            complement(33171..33258)
                     /locus_tag="ACHOQQ_RS00720"
                     /old_locus_tag="pgaptmp_000144"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33222..33224),aa:Ser,seq:tga)
     gene            33684..34802
                     /gene="hyaA"
                     /locus_tag="ACHOQQ_RS00725"
                     /old_locus_tag="pgaptmp_000145"
     CDS             33684..34802
                     /gene="hyaA"
                     /locus_tag="ACHOQQ_RS00725"
                     /old_locus_tag="pgaptmp_000145"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGU4468460.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34799..36592
                     /gene="hyaB"
                     /locus_tag="ACHOQQ_RS00730"
                     /old_locus_tag="pgaptmp_000146"
     CDS             34799..36592
                     /gene="hyaB"
                     /locus_tag="ACHOQQ_RS00730"
                     /old_locus_tag="pgaptmp_000146"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGU4468461.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            36611..37318
                     /gene="hyaC"
                     /locus_tag="ACHOQQ_RS00735"
                     /old_locus_tag="pgaptmp_000147"
     CDS             36611..37318
                     /gene="hyaC"
                     /locus_tag="ACHOQQ_RS00735"
                     /old_locus_tag="pgaptmp_000147"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGU4468462.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            37315..37902
                     /gene="hyaD"
                     /locus_tag="ACHOQQ_RS00740"
                     /old_locus_tag="pgaptmp_000148"
     CDS             37315..37902
                     /gene="hyaD"
                     /locus_tag="ACHOQQ_RS00740"
                     /old_locus_tag="pgaptmp_000148"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGU4468463.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLGAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            37899..38297
                     /gene="hyaE"
                     /locus_tag="ACHOQQ_RS00745"
                     /old_locus_tag="pgaptmp_000149"
     CDS             37899..38297
                     /gene="hyaE"
                     /locus_tag="ACHOQQ_RS00745"
                     /old_locus_tag="pgaptmp_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGU4468464.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            38294..39151
                     /gene="hyaF"
                     /locus_tag="ACHOQQ_RS00750"
                     /old_locus_tag="pgaptmp_000150"
     CDS             38294..39151
                     /gene="hyaF"
                     /locus_tag="ACHOQQ_RS00750"
                     /old_locus_tag="pgaptmp_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGU4468465.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            39285..40829
                     /gene="appC"
                     /locus_tag="ACHOQQ_RS00755"
                     /old_locus_tag="pgaptmp_000151"
     CDS             39285..40829
                     /gene="appC"
                     /locus_tag="ACHOQQ_RS00755"
                     /old_locus_tag="pgaptmp_000151"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HGU4468466.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQSFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40841..41977
                     /gene="appB"
                     /locus_tag="ACHOQQ_RS00760"
                     /old_locus_tag="pgaptmp_000152"
     CDS             40841..41977
                     /gene="appB"
                     /locus_tag="ACHOQQ_RS00760"
                     /old_locus_tag="pgaptmp_000152"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4468467.1"
                     /translation="MFDYETLRFIWWLLIGVILVAFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41990..42082
                     /gene="cbdX"
                     /locus_tag="ACHOQQ_RS00765"
                     /old_locus_tag="pgaptmp_000153"
     CDS             41990..42082
                     /gene="cbdX"
                     /locus_tag="ACHOQQ_RS00765"
                     /old_locus_tag="pgaptmp_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGU4468468.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            42162..43466
                     /gene="appA"
                     /locus_tag="ACHOQQ_RS00770"
                     /old_locus_tag="pgaptmp_000154"
     CDS             42162..43466
                     /gene="appA"
                     /locus_tag="ACHOQQ_RS00770"
                     /old_locus_tag="pgaptmp_000154"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGU4468469.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCP
                     QSGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43586..45766)
                     /gene="etk"
                     /locus_tag="ACHOQQ_RS00775"
                     /old_locus_tag="pgaptmp_000155"
     CDS             complement(43586..45766)
                     /gene="etk"
                     /locus_tag="ACHOQQ_RS00775"
                     /old_locus_tag="pgaptmp_000155"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGU4468470.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(45786..46232)
                     /gene="etp"
                     /locus_tag="ACHOQQ_RS00780"
                     /old_locus_tag="pgaptmp_000156"
                     /gene_synonym="yccY"
     CDS             complement(45786..46232)
                     /gene="etp"
                     /locus_tag="ACHOQQ_RS00780"
                     /old_locus_tag="pgaptmp_000156"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGU4468471.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(46220..47359)
                     /gene="gfcE"
                     /locus_tag="ACHOQQ_RS00785"
                     /old_locus_tag="pgaptmp_000157"
     CDS             complement(46220..47359)
                     /gene="gfcE"
                     /locus_tag="ACHOQQ_RS00785"
                     /old_locus_tag="pgaptmp_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGU4468472.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(47405..49501)
                     /gene="gfcD"
                     /locus_tag="ACHOQQ_RS00790"
                     /old_locus_tag="pgaptmp_000158"
     CDS             complement(47405..49501)
                     /gene="gfcD"
                     /locus_tag="ACHOQQ_RS00790"
                     /old_locus_tag="pgaptmp_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4468473.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(49501..50247)
                     /gene="gfcC"
                     /locus_tag="ACHOQQ_RS00795"
                     /old_locus_tag="pgaptmp_000159"
     CDS             complement(49501..50247)
                     /gene="gfcC"
                     /locus_tag="ACHOQQ_RS00795"
                     /old_locus_tag="pgaptmp_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4468474.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLAVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(50244..50888)
                     /gene="gfcB"
                     /locus_tag="ACHOQQ_RS00800"
                     /old_locus_tag="pgaptmp_000160"
     CDS             complement(50244..50888)
                     /gene="gfcB"
                     /locus_tag="ACHOQQ_RS00800"
                     /old_locus_tag="pgaptmp_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGU4468475.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(50995..51300)
                     /locus_tag="ACHOQQ_RS00805"
                     /old_locus_tag="pgaptmp_000161"
     CDS             complement(50995..51300)
                     /locus_tag="ACHOQQ_RS00805"
                     /old_locus_tag="pgaptmp_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4468476.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT        12754 a        12695 c        13548 g        12463 t
ORIGIN      
        1 ttttttaaga gtgactattt atgaaaactt gcataacaaa gggaattgtg accgtaagtt
       61 taacggcaat attactcagc tgctcatccg catgggcggc aggtaaaggc gggattggac
      121 ttgcagcaac acgtcttgtt tattcagaag gtgaagagca aatttcactg ggtgtacgta
      181 acaccagtcc ggatgttcct tatcttattc agtcatgggt gatgacccca gataataaaa
      241 aatcagcaga ctttattatt acaccaccgc tatttgtgct gaatccggct aatgagaatc
      301 tgttacgcat tatgtacatt ggtgcgccgt tggcgaaaga cagagaaact cttttcttca
      361 ctagcgtacg ggcagtccct tcaacaacga agcgggaaga aggcaatacc ctgaagattg
      421 ccacacaaag cgtcatcaaa cttttctggc gaccaaaagg tttagcgtat cccttaggcg
      481 aggctccggc gaaactgcgt tgcacttcgt cagctgacat ggttacggtc agtaacccaa
      541 caccttattt cattaccctg acagacctga aaataggtgg aaaggtagtt aaaaatcaaa
      601 tgatttcccc ctttgataaa taccaattct ctctgccaaa gggtgccaaa aatagcagcg
      661 taacgtatcg aaccatcaat gactacgggg cggaaacgcc gcaactcaac tgtaaatcgt
      721 aattcgtctt cagttaagag agcgagatgt atagaactca ccgacaacac agcctgttaa
      781 gctctggtgg cgtgccatcg tttattggtg ggctggtggt gtttgtgtcg gcagcgttca
      841 atgcacaagc tgaaacctgg ttcgatccag cctttttcaa agatgatccc tcaatggtgg
      901 ccgatttgtc tcgtttcgaa aaaggacaaa aaataacgcc aggggtttat cgtgtcgata
      961 ttgttctgaa tcagacaatt gtagatacgc gcaacgtcaa ttttgttgag ataacgccag
     1021 agaaggggat tgccgcctgt ttgacgaccg aaagcctgga tgcaatgggc gtgaatactg
     1081 atgcatttcc ggcttttaaa caactggaca aacaagcgtg tgcgctattg gcggagatta
     1141 ttccggatgc cagcgtaact tttaatgtga ataaactccg tctggaaatt tcagtaccgc
     1201 aaattgccat caaaagtaat gctcgtggtt atgtcccccc tgaacgttgg gatgaaggga
     1261 tcaacgcgct attactggga tattcattta gcggggctaa cagtattcat agcagcgcag
     1321 acagtgattc tggcgacagt tattttctga atttaaacag tggcgttaat ttaggcccat
     1381 ggagattgcg caacaattca acatggagtc gtagtagtgg ccaaaccgca gaatggaaga
     1441 atctcagcag ctatttgcaa cgggcggtta ttccactaaa aggcgaactg accgtaggtg
     1501 atgattatac tgcaggcgat tttttcgata gcgccagctt tcgtggtgtg cagctggcat
     1561 cagatgacaa catgctgcca gacagcctga aagggtttgc gcctgtagtg cgtggtatcg
     1621 ccaaaagcaa tgcccagata acgattaagc aaaatggtta caccatttac caaacttatg
     1681 tatcgcctgg tgcttttgaa attagtgatc tctattccac gtcgtcgagc ggtgatttgt
     1741 tggttgaaat caaagaagcg gacggcagcg tcaatagcta tagcgtgccc ttttccagcg
     1801 tgccattact ccagcgtcag gggcgaatca aatacgcggt gacactggcg aaatacagga
     1861 ccaatagtaa tgaacagcag gagagcaaat ttgcccaggt cacgttgcag tggggcggac
     1921 cgtggggaac gacctggtat ggtggtggac aatatgctga atattaccgt gctgccatgt
     1981 ttggtctggg atttaacctc ggcgatttcg gagcaatttc gttcgatgcg acccaggcga
     2041 agagtacgct ggcagaccaa agcgaacata aaggtcagtc atatcgtttt ctgtatgcca
     2101 aaacgctcaa ccaattgggc actaattttc aattgatggg ctatcgctat tcgacgtcgg
     2161 gtttctacac cctttccgac accatgtata aacatatgga tggctacgaa tttaatgacg
     2221 gtgatgatga agatacgccg atgtggtcgc gttattacaa tttgttttac accaaacgtg
     2281 gcaaactgca ggtcaatatc tcccagcaat taggcgagta cggttcgttt tatttaagtg
     2341 gtagccagca aacttactgg cataccgatc aacaggatcg gctattacag tttggctata
     2401 acacgcaaat taaagatctc tcgctgggga tctcctggaa ctacagtaag tcccgtggtc
     2461 aacctgatgc tgatcaggtg tttgcactaa atttttccct gccgctcaat ctgttgctcc
     2521 ccagaagtaa tgatagctat accaggaaaa aaaattacgc ctggatgacc tctaacacca
     2581 gtatcgataa cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg
     2641 atggtaacct gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg
     2701 gtagcgccag catagactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca
     2761 gcgataacgg cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt
     2821 cacagggcat taccctgggg caatcgctgg gggaaactaa cgtgctgatt gcagcaccag
     2881 gcgcagagaa tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata
     2941 ccgttgttcc ttatgccact tcttatcgtg aaaattgaat cgcacttgat gcggcgtcgt
     3001 taaaacgtaa cgtggatctt gaaaatgcag tagtcaacgt ggttcccacc aaaggggcgt
     3061 tggttctggc ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acaacaaagc
     3121 agggtatacc gctgcgtttt ggcgcgatag cgacgctgga cggggtacag gctaatagcg
     3181 gcataattga tgatgatggc tcgctctata tggcgggttt accggcgaag ggaacaataa
     3241 ccgtacgctg gggcgaagca cccgatcaaa tttgtcatat cagttacgag cttaccgaac
     3301 aacaaattaa ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa
     3361 atgcgtgtca ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac
     3421 cgctgcttat agcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt
     3481 tggcgttaaa accctggagg aagaagaaaa tactgcaggg gtagttaaag acaaatttta
     3541 tcagtggaac gaatcgaatg attattatgt ttcctgtgat tgcgataaag acaatgtcag
     3601 aaatggtcga tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta
     3661 caaaattaat gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac
     3721 tgcggttcct ttcgaaaacg tgggcacagg gggggatacc cgatggcata tttgcgaccc
     3781 tggcggtcaa cgtttaggcg gccagggggc tagcggtaat agcggtagct tttccctgaa
     3841 aatattgcag ccgttcgttg gctcggtcgt cattcctcct atggcgctgg cgcgattatt
     3901 tgaatgctac aacatacctg caggtgattc ctgcacgact acaggtacac cggttttagt
     3961 gtattacctg tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac
     4021 aatcgaggtt gatctgggcg acgtatttgc ggctaacttt cgtgttgtag ggcataagcc
     4081 tcttggggcc agaacggcag aacttgcaat tccggtcagg tgtaacacgg gaaacgcggg
     4141 gttagttaac gtcaacctga gtctgactgc aacctcagac cccagctatc cccaggcgat
     4201 taagacgtca cgtccaggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc
     4261 cccggctggt ggaacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat
     4321 aaatgtctat ccagtcagca cgacaggtgt accaccagaa accgggcgat ttgaagccac
     4381 ggcaacggtg agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatattt
     4441 cagtgcgtta tcttgttctt tagcattctt aacatccatg tcgggatggc tgggcctgaa
     4501 caagttagta tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa
     4561 agtgcattac aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat
     4621 atcgtaagca ctgctgctga tttgaatatc gatatcaccg gttgtgccgc tggtattact
     4681 ggcgcggacg tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta
     4741 actgacacag gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg
     4801 caaagtcagc aagaaatccc gctcaatcag gtccagcctc ttacgccctt aaaagccggg
     4861 gataacacac tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt
     4921 aatgcgacgg cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg
     4981 gataatctgg attctgttct tactgggatt aacggggggt tgtgagctat ttgcccatga
     5041 tggcacggtc aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag
     5101 caagcaaatc aatgtgcagt taggcgatgt ctcactgaca cgtttttctc atgggaatta
     5161 tggcccggag aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt
     5221 tgacgtcacc ttttcaggaa cccccgacgg agtgcagagc gaaatgttgt ccattgaaag
     5281 tggtacggat gcagccagcg ggctggcaat tgcgattctg gatgatgcga agatattgat
     5341 tccgcttaac caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt
     5401 ttatgcgcaa ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc
     5461 gacatttgta cttcattatg actaatacgt ggaacagatt ggcgctcttg attttcgccg
     5521 ttttatcgct gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat
     5581 ttatctttcc ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat
     5641 cctggcttat taacagtaaa atcaaccgcc caacgcgttg ggccgggggt gaagcgtcga
     5701 cagtgccggc accattactg gccgctccgc cacttattct cctgaagccc ggtacgacag
     5761 gcacgttgcg cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat
     5821 ttgagttaag cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg
     5881 cgatgcgctc ggtatttaaa ctgttctggc gacccgaagg tttgccgggc gacccgctgg
     5941 aagcttatca acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa
     6001 cgccttacta cattaacctg attcaggtaa gcgtaaatgg taaagcgtta agtaatgtgg
     6061 gagttgtgcc gcctaaaagc ctgcgtcaga caagctggtg tcaggcaata gcaccgtgtc
     6121 atgttgcctg gcgggcgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc
     6181 tgccctgaaa caggttcgga aaacgtttgc gttttttttg ccgcaggtca attccctttt
     6241 ggtccgaact cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat
     6301 gtactacccc ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt
     6361 tacttttcag caattacgcc gcattaccgg aacaccgttt gaagcactgg tgcggcagaa
     6421 agtgcctgcg aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc
     6481 agccggtctt gataaagacg gggagtgcat tgacgcgtta ggcgcgatgg gatttggatc
     6541 gatcgagatc ggtaccgtca cgccacgtcc acagccaggt aatgacaagc cgcgtctctt
     6601 tcgtctggta gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga
     6661 taacctcgta gagaacgtaa aaaaagccca ttatgacggc gtcctgggta ttaacatcgg
     6721 caaaaataaa gatacgccag tagagcaggg caaagatgac tatctgattt gtatggaaaa
     6781 aatctatgcc tatgcgggat atatcgccat caatatttca tcgccaaata ccccaggatt
     6841 acgcacactg caatatggtg aagcgctgga tgatctctta actgcgatta aaaataaaca
     6901 aaatgatttg caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga
     6961 tctttctgaa gaagaattga tccaggttgc cgatagttta gttcgccata atattgatgg
     7021 cgtgattgcc accaatacca cactcgatcg ttctctggtt cagggaatga aaaattgcga
     7081 tcaaaccggt ggcttaagtg gtcgtccgct tcagttaaaa agcaccgaaa ttattcgccg
     7141 cttgtcactg gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt
     7201 tatcgctgcg cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt
     7261 tatttttaaa ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg
     7321 aattcctctt tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc
     7381 actgttgctt atttagacat tttgtacttt tattgttgag gttattaagc gaagcgacaa
     7441 tggattgtgt tgctgcggtt ttataaggtt ggggagaggc agatgcgaat taaaccagac
     7501 gataactggc gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat
     7561 ggtatgctat ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc
     7621 gcaggctttt gcgttgacga cgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat
     7681 tttaatttat ctaaagaaca aaaagccgaa ctggtgctga atgcactggt agcaattcgc
     7741 tatctcaaac cgcaaatgcc gaaaagctgg cattttgttt cccatggtga aatgtgggtg
     7801 cccatgccgg gggatgcggc ctgcgtttgg ttgagcgaca ctaacgagca agtgaattta
     7861 ctggtcgttg aatctggcga gaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt
     7921 attgcgggtc gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa
     7981 ccgcaggtta atgttgacag cttcagcctc gaacaggcag tctaacgcgc caacttaagt
     8041 gcagtcttcg gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt
     8101 ttcagcggcg tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg
     8161 cggcaagaat aagggatacg aattccctga ttttctaatt gctccagcaa cacctgttgg
     8221 ttatttccac gaaatgcctg tccctgccag tcaatatcta cattggcgtc cggttgttgc
     8281 gtgatgttgg cagtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgtc
     8341 agaatttcca cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga
     8401 ccaaaatcga catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt
     8461 tcgcctgccg gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta
     8521 caaggtttaa ccacatcaaa caccacatca ccaatgcgaa tcactttcca actatcttct
     8581 tcccaggctg acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg
     8641 gccggacaac gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag
     8701 ccatcagcaa atgacagggg tacagtgttg tggcgtttca cgcgccgggt catttgtggc
     8761 cccacccagc gtaattgcac ttcgcgggag aaaaatccac ttaaccattt gttgatcgct
     8821 tctggcgcaa ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg
     8881 gcgaaatcag caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca
     8941 tcatgcacag gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac
     9001 gtaccgtcag gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc
     9061 agagcatgtg taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt
     9121 aatgtcgcca cggtgtggtc ctcaaatgaa aataagccct caactttatg acatgacgcg
     9181 cttattagct ataatgcgca acaattttct tagcgcctgt cccattaggc tattttattt
     9241 gtcagtttgg ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct
     9301 ccgcgctgtc catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga
     9361 gtaaaagtga cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt
     9421 taaaaactga actggaaaac ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc
     9481 atttcaaggg cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc
     9541 gtattatgtt gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg
     9601 ttcaggcgat caactggaca gagatgttta atcctggcgc gaccttcgct gtccacttca
     9661 gtggtttgaa tgacacaatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcaa
     9721 tcgtcgatgc tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg
     9781 atatccgcgt taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg
     9841 gtgatggttt acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa
     9901 ccctggcagc cgctattgtg atgcgatccg gctggcagcc aggaacaccg ctgctcgatc
     9961 cgatgtgtgg ttccggtacg ttgctgattg aagcggcgat gctggcgacc gatcgcgcac
    10021 caggcttgca ccgtgggcgt tggggcttta gcggctgggc gcagcatgat gaagctatct
    10081 ggcaggaagt gaaagcagaa gcgcaaactc gcgcccgtaa aggcctggct gagtatagct
    10141 ctcattttta cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc
    10201 gtcttgcggg gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca
    10261 atccgctgcc gaaagggccg tacggcacag tgttgagcaa cccgccatac ggggaacgtc
    10321 tggacagcga accggcgctg attgcgctgc atagcctgct gggtcggatc atgaaaaacc
    10381 agtttggtgg ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcttgc
    10441 agctgcgtgc agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa
    10501 attaccacgt tgccgaaagc actccagaca gcaaaccggc gatggtagcg gaagattacg
    10561 ccaaccgcct gcgtaagaac ctcaaaaaat tcgagaagtg ggctcgccag gaaggaattg
    10621 aatgttaccg cctgtatgac gccgatctgc cggaatataa cgttgccgtt gaccgttatg
    10681 ccgactgggt ggtggtgcag gagtatgcac cgccaaaaac tattgatgcg cacaaagcgc
    10741 gtcagcgtct gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca
    10801 aactggtgct gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg
    10861 agaagggcga gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg
    10921 attatctcga tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga
    10981 tgagcaaagg caaagatttc ctcaacctgt tttcttatac cggcagcgcc accgtgcatg
    11041 cgggattagg cggtgcacgc agcaccacca ccgtggatat gtcgcgtact tatctggaat
    11101 gggcagaacg caacctgcgt ctgaatggcc tgaccgggcg tgcgcatcgc ctgattcagg
    11161 ccgattgcct ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc
    11221 cgccaacctt ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc
    11281 tggcgctgat gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga
    11341 acaacaaacg tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac
    11401 aagaaattac gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact
    11461 gctggctgat taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc
    11521 atggctgtcg ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa
    11581 cgaacgtgtt tgtctggtgg gccgcaacgg cgcaggcaaa tcgacgttaa tgaaaatcct
    11641 caaccgtgaa caagggctgg atgacggtcg cattatttac gagcaggatt tgattgtagc
    11701 gcgtctgcaa caggatccgc cgcgtaacgt tgagggtagt gtttatgatt tcgttgccga
    11761 aggcattgaa gaacaagcgg aatacctgaa acgctatcac gatatttcgc gcctggtgat
    11821 gaacgacccg agcgagaaaa atctcaacga actggcaaag gttcaggaac agctggatca
    11881 ccacaacctg tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga
    11941 tcctaacgtt gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg
    12001 cgcgctggtg agcaatccgc gcgtattgct gctggacgaa ccgactaacc atctggatat
    12061 cgaaaccatc gactggctgg aggggttcct taaaaccttc aatgggacga ttattttcat
    12121 ctcccacgac cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg
    12181 caagctggtg acctatccgg gcaattacga tcagtacctg ctggaaaaag aagaagccct
    12241 gcgcgtggaa gaactacaaa atgccgagtt cgatcgcaaa ctggcgcagg aagaagtgtg
    12301 gattcgccag gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa
    12361 agcgatgcgt cgcgagcgtg gcgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt
    12421 ggaagaggcc agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt
    12481 tgacggaaag caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc
    12541 cctgattggg ccgaatggct gcggcaaaac cacgctgctg aaactgatgc tcggtcagct
    12601 tcaagcggac agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca
    12661 gcaccgcgcg gaacttgatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca
    12721 agaggtgatg gttaacggca agccacgcca cgttttgggc tatttgcagg acttcctgtt
    12781 ccatccgaaa cgggcgatga cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt
    12841 gctgctggcg cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa
    12901 cgatcttgat gtcgaaacgc tggagctgct ggaagaactg atcgacagct accagggcac
    12961 ggtattgctg gtaagccacg atcgtcagtt tgttgataac accgttacag aatgctggat
    13021 cttcgaaggc ggcggtaaaa ttggtcgtta tgtcggcggt tatcatgacg cccgtggtca
    13081 gcaagagcag tatgtggcgc tcaaacagcc tgcggtgaaa aaaaacgaag aagccgccgc
    13141 gccaaaagca gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga
    13201 actggagcag ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac
    13261 gcaggtggcg gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgctcgc
    13321 tgatatggct gccgcagaac aggaactgga gcaagccttt gaacgctggg agtatcttga
    13381 agcgttaaaa aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac
    13441 ggtagaattg cctgatgcac tgggtttatc aggcatgaaa accaacactt tgttagtcat
    13501 ctgtaaaata gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca
    13561 catcctgtgc tcgcagtgtg acatgctggt agcgttaccg cgccttgagc atggtcagaa
    13621 agcggcatgt ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc cccggcagcg
    13681 tcctaccgcc tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt
    13741 tgtgaatatg aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt
    13801 gcttttttct gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt
    13861 tcccgcgttt tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggttcg
    13921 tttaaaagag caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat
    13981 tttcctcgcg ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggcgt
    14041 aggcagcagc tttctcccct ggtgtttatt ttgtgtcctg caactgcgtg cttttcagtg
    14101 cgttgatcgt cgctggttat gggacgatat cgccccgatg ccagaactgc gccagccgct
    14161 aaaaccaggc gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat
    14221 ccttcctgct gatgaacccg tgtgcccgcg ctgtggtact aaagggtacg ttcgacgtag
    14281 aaacagcctg caatggacac tcgcgctgct tgttacgtcc atcatgctgt atcttccggc
    14341 taatattttg cccatcatgg tgacggattt attaggctcg aagatgccat cgacgattct
    14401 cgctggggtc atcctgttat ggagcgaagg ctcttatccc gtcgctgcgg tgatcttcct
    14461 tgccagtatt atggtgccaa cgttaaagat gatcgccatc gcgtggctgt gttgggatgc
    14521 caaagggcat ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt
    14581 tgtaggccgc tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg
    14641 tatgggaggt ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt
    14701 cataatgaca atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc
    14761 tgaatcagag catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc
    14821 agaaagtgaa gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg
    14881 cctgggttct tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga
    14941 atgcggaagg aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg
    15001 tggttgaaag cgccacactc gctgatgatt tgacacacgt tgaaatcaaa gcgcggctga
    15061 attccggtat ggaaaaattg ctgcataaag ataccgtctt ttgggtggtg aaaccgcaga
    15121 ttggtcgaga agggattagc ggtctgggaa ccctgctgtc tggggtttat atcgaactgc
    15181 agccaggcgc gaaaggcagc aaaatggata aatacgattt gctggactcg ccaccgttgg
    15241 ccccgcctga tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct
    15301 cgccaggaga tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct
    15361 tcgatacgca aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac
    15421 tggtgaccag caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag
    15481 cgggaatgcg tgtggagatg ggctcattga caacgctgct gagtggcggt gtcagctttg
    15541 atgtgccgga aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt
    15601 atgatgatca gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt
    15661 tctttaaaga ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc
    15721 gcctcggtac cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg
    15781 atgattaccg tattccggtg ctgattcgta tcgagccaga gcggctgaaa atgcagcttg
    15841 gcgaaaatgc ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat
    15901 cgctgaaaac cggaaacctg gtcactggcg cactgtatgt tgatctcgat ttctatccaa
    15961 atacgcctgc aataaccggt attcgtgaat ttaatggtta tcagattatc ccgaccgtta
    16021 gcggcggcct ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac
    16081 tgccattgaa tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa
    16141 tgaaaaacct gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc
    16201 agcagttgcc gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct
    16261 tccagcccgg ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg
    16321 tgttgcgaga actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat
    16381 ttgaagcgaa ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct
    16441 agtgacgatt gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta
    16501 ttaccagtta cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgcct
    16561 gttatgggta gagcaggtcg ctgtacctga ctatctggcg gggaatggtg tggtttatca
    16621 aaccagtgat gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca
    16681 acagttgcgc aacaccctgg tagccaacct gagcacgcaa ctgcccggct gggtggttgc
    16741 ctcccagcct ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg
    16801 tcgctatgat ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact
    16861 gatcaaacgt ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt
    16921 taaagtgctg gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg
    16981 tctaccttaa ttataaagat ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt
    17041 ttttttgttt ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca
    17101 tgcccgtgta gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg
    17161 attcgcggta ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca
    17221 gaaaccagta tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc
    17281 acatcaacgt ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca
    17341 gacgctgaat caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt
    17401 agtaatggcc tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt
    17461 ttgatactct gtctgatgat aatcagaagg cagacgtatc ctggaacaga ccgactttca
    17521 ggtcgctggc ggtatagatc agacgaccat caaccagcac ttcgccatct gccagaccca
    17581 taatcagacg acgattaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg
    17641 tcggcagtac ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt
    17701 cgccgcccag ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc
    17761 atcccggcat aaccggatcg ccaataaagt ggcatccgaa gaaccacaga tccggattga
    17821 tatccagttc tgcttcaaca taccctttgt tgaagttacc acccgtttcg gtcattttga
    17881 ccacacggtc catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca
    17941 gttcaccgcg accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca
    18001 tgttctctgt aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa
    18061 caagtccgat cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg
    18121 acgtccttcc tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt
    18181 ttggccttcg ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc
    18241 tactgcccag atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact
    18301 taaatggcga acgttagcag tggggataat gacaccttgt ttacccgtta actcacgttg
    18361 ctggcaaata gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctgggcgcg
    18421 accgaactga tcgactgaac ctgtgatagc gatactctga ttcaccggca catcggcgag
    18481 ggcgcttatc agagcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta
    18541 tgactgctca aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga
    18601 catcaggaac gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc
    18661 tttgcgttcg atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga
    18721 aggttcgcca aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg
    18781 cccgatgcgt tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat
    18841 gcgttcagcg aggaaacctt cgcgccattc acgctgctgt agcattaagt ttagctgctc
    18901 gccggagaag gtgtcgccat cacacaggga ggcgacttct ttacactggc ggaggatcca
    18961 ctgcgggcta agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag
    19021 tatcggccag gcatccgctc ccggtgcagg taagtgatta tgtctggcgg taaatgtcac
    19081 ccagcgacac cactggctta ctgactccgc atcgacaatc tggagagtat cttcaaattc
    19141 gctataaata gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc
    19201 gcgttcgcct accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag
    19261 agggcgcgac tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag
    19321 ccgcatccac agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc
    19381 atttgcctga tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca
    19441 gccaaagagt tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa
    19501 attatcatct gcactcactg cgttacgtaa gcggatggtg tggccggaaa cctcatagtg
    19561 accgcccacc agttggcctg catcactttg tagcgcacgt gccgcatcgg caataagatt
    19621 cagatactca gactcttccg gggccttcgc cagcataaaa gaggaggatg ctcgcgtatg
    19681 cagcaactgc tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt
    19741 ttcgtcaatc aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag
    19801 gtcacgccat gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc
    19861 cgcgattata ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc
    19921 atgggcgtta aagctcacag gatggagatt cttttcttca ctggcctaaa aagctgatat
    19981 tctgtaaaga gttacacggt aacattgaga tcgctatgaa atatcaacaa cttgaaaatc
    20041 ttgaaagcgg ttggaaatgg aagtatctgg tgaagaagca tcgcgaaggg gagttaatta
    20101 cccgttacat agaagccagt gccgcccagg aagccgtcga tgtgttgctc tcgctggaaa
    20161 atgaacccgt gctggtaaat ggctggattg acaagcatat gaatccggaa ctggtcaatc
    20221 gcatgaagca aaccattcgg gccagacgga agcgccattt taatgcggaa caccagcata
    20281 cgcgcaaaaa atcgatcgat ctggaattta ttgtctggca acgtctggct ggtcttgcgc
    20341 agcgtcgcgg taaaacactt tctgaaacga ttgttcagct gattgaagat gcggaaaaca
    20401 aagagaaata cgcgaataaa atgtcttctt tgaagcagga tctgcaggca ttgctgggta
    20461 aggaataact gatgaaactc agttcaattt actaaaggcg aaaaaaaacc ccgcagctgc
    20521 ggggtttttt accagacgag aacttaagcc tgcggctgag ttacaacgtc tttgatacct
    20581 ttaacttcga tctcaacgcg acgatccgga gccaggcagt cgatcagtgc agcacgctgt
    20641 ttcacgttgt cacaggtgtt gccagtaacc gggttggatt cgcccatacc acgtgcggag
    20701 atcttgtctg ccgggatacc tttggagatc aggtaatcaa caacagactg agcacggcgc
    20761 tcggacagac cctggttgta agcgtcagaa ccgatgcggt cggtgtaacc cagaacaact
    20821 acggaaccgt ctttcggatc caggttgctc agctggctgt acagctgatc cagagcagcc
    20881 tgaccttccg gtttcagggt tgctttgttg aagttgaaca gaacgtcaga cttcagagtg
    20941 aagtgcttgg tctgtacttc cggtgccgga gctggagccg gagcaactac cggagctgct
    21001 tcgccctgac cgaaacggta ggaaacaccc aggctcagca ggccgttgtc cggacgagtg
    21061 ccgatggtgt gtgcgtcacc gatgttgttg gtccactggt attccagacg ggtagcgatt
    21121 tcaggagtga tggcccattc aacaccacct gcgaataccg gagaaacgcc ggtatcgtgg
    21181 tttttctcag attcacctgt cacattgttg tgagctttgg tgtctgcacg ccaaaccata
    21241 ccacccagac gagtgtacac gtccaggtcg tcagtgattg ggtaacccag tttagcggtc
    21301 aactgaacgc cctgagcttt gtatgcaccg ttttcaacgc tgcctttgta cggcatacga
    21361 cctaaccagt cgtaacccat ttcaaagcca acatacgggt taacctggta accaccaaaa
    21421 gcaccagcgc ccagttggtt ttcatgggtc gggccattgt tgtcgatgaa accagtgtca
    21481 tggtactggg accagcccag tttagcacca gtgtaccagg tgttatcttt cggagcggcc
    21541 tgcgctacgg tagcgaaacc agccagtgcc actgcaatcg cgatagctgt ctttttcatt
    21601 ttttgcgcct cgttatcatc caaaatacgc catgaatatc tccaacgaga taacacggtt
    21661 aaatccttca ccgggggatc tgctcaatat taactctacc gatatcttcg gcttatgccg
    21721 agcacccctg gcgatgtaaa gtctacaacg tagttgaaaa cttacaagtg tgaactccgt
    21781 caggcatatg aaaaaaaagt cttgtataag gtatgtttaa tcttttttgt cagcgacaat
    21841 ttacagaaga aaatcgcgga aaccgcttca gacaagcctc cgcaaggaaa attaatcacg
    21901 actgaaagca ttggctggac gacaaaaaaa gttccaggat taatcctaaa tttacttaat
    21961 gatacaaatt agagtgaatt tttagcccgg aaagttgtct cgttgcgtga gaggatgcgc
    22021 ttaccggacg cataataaac cccatagcgt taccttcatt tgccgcatca acaagttcag
    22081 catgctcttc ttcagtcaaa tcatctgcca accaaccgat caccacactg taattgcccg
    22141 tgcgtaaagc gcgaaccatt gactccacag tgtggcaagg ggggagctgg ctaatctgca
    22201 ttactttcgt taagggtagc ccagatgcct gaacccattc ccgactcagt ttttgttgcg
    22261 gtgttaacca gagttgccag cgcgattgct gaccgagttg ctgtaacaat ggcaacagta
    22321 gaagttgcgt catcatgggc tgatcttcgc gatagacaac ttcactgata agcccggctg
    22381 tagtgttttc cgtagagaca cgcgcaattt tacttgctgc ggatgagaac gacgaagaac
    22441 gatgtgcata gcctgaagtg tacataatca atccagcccc tgtgagttac tgtatggatg
    22501 tacagtacat ccagtgacga caaagatcaa ccctattttc ggaaagagcc tcgcaaattt
    22561 tgtcgttggt gacgggaaaa cataaattaa tcttgcccct aaagaataag ttgcctattt
    22621 tcgtatttaa cggatccgtt aatgtgaacc attcttttat gttatgattt taaaaggaat
    22681 tttatgaaaa gcctctccta taagcggatc tataaatcac aagaatacct ggcaacgttg
    22741 ggcacaattg aataccgatc attgtttggt agttacagcc tgaccgttga cgacacggtg
    22801 tttgcgatgg tttctgatgg tgagttatat cttcgggctt gtgagcaaag tgcacagtac
    22861 tgtgtaaaac atccgcctgt ctggctgaca tataaaaagt gtggccgatc cgttaccctc
    22921 aattactatc gggttgatga aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg
    22981 aaatattctc tcgatgcagc gctgaaagag aaaagcacgc gcaatacccg ggaaagactg
    23041 aaagatttgc ccaatatgtc ttttcatctg gaagcgattc tcggggaggt ggggattaag
    23101 gatgtacggg cgttacgtat acttggggca aaaatgtgtt ggttgcgact gcggcagcaa
    23161 aacagtctgg tgacagaaaa gattctgttt atgcttgaag gtgccattat cggcattcat
    23221 gaagctgcgc tcccggtggc acgccgccag gagcttgcag aatgggctga ctctcttacg
    23281 ccgaaacagg agtttcctgc ggaacttgag taatctggcg ttgcagacga ccaatctcag
    23341 gcagtaatgc aatcaataac ccaacttgtt gtacgaccag aggttctttg ctgtctgccc
    23401 gtggttcaag ttgctgcatc cgctgtttca ggccagctaa tgcctgattg acgcgttctt
    23461 catcagcagg ttgatgatgt aacgcgtcat caacatagca aactgcgtca tcaagaaatg
    23521 ccagaatttc aggattagtt aactgctccc ggtgagcacc gagggctgag atatagctgg
    23581 taaacgtatg gttaaggcac agcaaccgaa acgcggcttc gcgaatttgc ggggtaacgt
    23641 tcggttcgct ggacatattt gataccaccg acgccagttc agcatcacgg ttgtgtgcat
    23701 cgcggcgggc aatacgatac gccagacggt tatcacgccc ctgatggtat tgctccagta
    23761 tggcatcgag atagcgacag ttggcctctg tggcgcgttc gagcatgcgc ggcagattgc
    23821 gaaactgcca gtcaggccag atgtagctca ctgccgccca cgcaatggca caaccaatca
    23881 gcgtatcgat tacgcgaggt aacgctactt caaaaccttc acccagtaag ttaaaacaca
    23941 gtagcaccaa aagtgtgatg aacatcgttg catgagcgta ttgcacgtta cggaaggcaa
    24001 aaaagagcac gccggtaata accagcagca ccagctgccc ttccagtgat ggcacaaacc
    24061 acagcacagg aatgccaatg gcgataccta ccagcgtacc aataatcctt aacttcaggc
    24121 ggtggcgcgt ggcgttatag tttggctggc agacaaacaa acttgtcagc aagatccaat
    24181 acccgtgatg cattccggtt atttgaatga tggcgtagcc gaagcacaac accagcgaca
    24241 ttcttaccgc atgacggaag agggcggatt ccggcgtgaa gtgacggcta agacgcagcc
    24301 agatatcact caatccgtgc gggctgtcat cagcgagctc attttcgtca ttattatggg
    24361 gcagtgcctg ggcctgttct gattcaattg ttgccagttg ggcatcaatg gcgcgtaaat
    24421 tgttcagcaa aaatcccagt gttttgagta aatcggcagg cgcgccgtta tcgcgcatcc
    24481 gctccagggc agcatcaata tgcgtaaaag cgcgctcaaa atgcggatca tgttgataag
    24541 gctgacgcag caaaatacag cgtgacagtt gctggcacgc ctggccctgc atcgacatca
    24601 gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg ttcacgcaat gtttgatact
    24661 gaatatgaga agagctggca cgctcgtgaa tatcctgtgc gacaaagtaa taatgcagcg
    24721 tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg ggtcagcagc gagagtttcg
    24781 tctgattcaa tgtcgccatc agctgaccgt tggcgagagc caaatcgtac agcggtgcct
    24841 ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt gagctcaaga taacgcgcca
    24901 gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg gaccgggaac agcagatgac
    24961 caataagtgt caggacgttg taccagacgg caccggccag cagatacatc ggctgctgat
    25021 accagtgctc atacagtgat gttcccaaca tagtgtaaat agcgatcagc aatgcaccga
    25081 aggcaattgt tgcatagcgt tgacccagac cgccgagcaa aatgaagccg ctggtagaga
    25141 gcgttaagcc aatcgcaaat agccagggcc agggaaacag caattctact gaggccgagg
    25201 cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa acgtcccgcc agtcggtcat
    25261 cgagatcggt cagcgccgct gccaccatcc ccagcgtcag cggaatcgtc agttttacat
    25321 cacccagcca ccacggaaac gctgtggttc cacaaagcgc aataaaaata cgcgcgtaat
    25381 acagccaggc gctgttccag gtatagcgtt tgagcaaagg acttagcata taggccatat
    25441 tagtggatta ttcaaaacga cgacgtgcat tggcttcacg cgcagcttgc gctgtttcta
    25501 ccgataccac gcgacgacca accggccata gtgcaatagc ggcaatttta aagttcgcaa
    25561 taccgacagg aatgccaatg atggaaatac attgtgcgat gcccgttgca atgtgcatca
    25621 ggcataacca ccagccaaag aaaatcagcc agaaaatatt caataccgta ccgccagtat
    25681 tcagcagcac atttttgcca gccgggttca gttcatcgac atgaatagct tcattgccat
    25741 aaggcaccag agacagttta gtgatctccc agcaggatcg tgtcagcggt aaggtaaaaa
    25801 tcagcacaat actgaccaga gtcgccaaca gccagcccag agtggtggca aatccgccaa
    25861 gcacaaagtt tagaatgttc aaaacggtac gcataaaacc tcgctttact gtggttttca
    25921 gtaatgggcg gcaattgtaa cgtttttttg ggctggagca cgttttctct gacggttaca
    25981 ctgataagaa ataatttcgt gtggatctac agagtcatgg aactgaaagc gacaacgctt
    26041 ggaaaacgtc tggcacagca cccttacgat cgggcggtga tcctcaatgc cgggattaaa
    26101 gtctccggcg atcgccacga ataccttatt cctttcaatc aattactggc gattcactgt
    26161 aagcgcggtc tggtatgggg cgagctggaa tttgtactgc cggacgaaaa agtggtgcgt
    26221 ctgcacggta ccgaatgggg cgagacgcag cgtttttacc atcatcttga tgctcactgg
    26281 cggcggtgga gtggcgagat gagcgaaatt gcgtctggtg ttttacgcca gcaactggat
    26341 ttgattgcca cgcgcactgg agaaaataaa tggctgacgc gtgagcaaac ctctggcgtt
    26401 cagcaacaaa tccgccaggc tttgtcggcg ttgccgttgc cggttaaccg actggaagaa
    26461 ttcgataact gccgtgaggc gtggcgtaaa tgtcaggcct ggttgaaaga tattgaaagc
    26521 gctcggttgc agcataacca ggcgtatacc gaagccatgc ttaccgagta tgcggatttt
    26581 ttccgccagg tcgagtcttc accgctgaat ccggcgcagg cccgggcagt cgttaatggc
    26641 gagcattctc tgttagtgct ggcaggtgca ggaagcggaa aaacgtcggt gctggtggcc
    26701 cgtgcaggct ggttgctggc gcgtggtgaa gcgtcccctg agcaaatttt attgctggcg
    26761 tttggtcgca aagccgctga agagatggac gagcgtattc gcgaacggct acataccgaa
    26821 gacattaccg cacgcacgtt tcatgcgctg gcgctgcata ttattcagca gggcagcaaa
    26881 aaagttccga tagtcagcaa actggaaaat gataccgctg cccgtcatga actctttatt
    26941 gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg cgaagggctg gcggcaatgg
    27001 ctgacggaag aaatgcagtg gtcagtgcca gaaggtaact tctgggatga tgaaaaatta
    27061 cagcgtcgtc ttgcctcacg cctcgatcgt tgggtaagtc tgatgcggat gcacggtggt
    27121 gcacaggcag aaatgattgc cagtgcaccc gaagagattc gcgatctgtt cagtaaacgt
    27181 atcaagttga tggccccgtt attaaaagcc tggaaaggtg cgctgaaggc agaaaacgct
    27241 gtcgattttt cgggccttat tcatcaggcg attgtgattc tggagaaagg tcgctttatc
    27301 agcccgtgga agcatattct ggttgatgaa tttcaggata tctcgccgca gcgggcagcg
    27361 ttgttagcgg cattacgcaa gcaaaacagt cagacgacgt tgttcgccgt tggtgatgac
    27421 tggcaggcga tttaccgatt cagcggtgcg caaatgtcgc tcaccaccgc tttccatgaa
    27481 aactttggtg aaggcgaacg ctgtgattta gacacgactt accgttttaa cagtcgtatc
    27541 ggtgaggtgg caaaccggtt tattcagcag aacccaggcc agctgaaaaa gccgctaaac
    27601 agcttaacca atggagacaa aaaagccgtc acgttattgg atgagagtca acttgacgct
    27661 ttgctggata agctctctgg ttatgccaaa ccggaagagc gcattctgat cctggcgcgt
    27721 taccatcaca tgaggcctgc cagcctggaa aaagcggcaa cacgctggcc gaagttgcaa
    27781 atcgacttta tgaccattca tgccagcaaa gggcaacagg cggattacgt catcatcgtt
    27841 ggcttgcagg agggaagtga tggttttccg gctgcggcgc gggagtcgat tatggaagag
    27901 gcgctactgc caccggttga ggatttcccg gacgctgaag aacggcggtt aatgtacgtg
    27961 gcgctgaccc gggcacgcca tcgggtatgg gcactgttta acaaagagaa tccctctccc
    28021 tttgtggaaa tactgaaaaa tctggatgtg ccggtggcga gaaaaccgta agaaacaggt
    28081 ggcgtttgcc acctgtgcaa tattacttca gacggtccgc gagataacgc tgataatcgg
    28141 ggatcagaat atcgaccgcg tcgttgaaat gcggcgactg gataatgaag tctgccgttg
    28201 ccacgttggt ggcgaccgga atgttccata ccgtcgccag acgcagcaag gctttcacgt
    28261 caggatcgtg cggcacggca tttagtggat cccagaagaa aatcaataca tcaattttcc
    28321 cttctgagat caatgcgcca acctgctggt caccccccat tgggccactc aacatcgcgt
    28381 tgacgttcat gccggttgcg cgggaaatca agttaccggt agtgcctgtt gcatacagta
    28441 cgtgttgttc cagtaacggt tgatgtcgtt ccacccagct catcagcatt tgtttgcagt
    28501 gatcgtgtgc taccagcgca atatgtttcc gcgcaggtaa agtgcgagtc gtcagttcca
    28561 taatgtacat ccgtagttaa ctttcctaca gattactgta agcacttatc gctgcaagat
    28621 aaagaccgaa aaagcctgcg cacaggcaca aaaatctcag gaagatggtt gtttttccgc
    28681 ccattgcagg aaagtatttc gcgtttgtgg gtcagccagt ttaaaccaat acttcagccg
    28741 ttgttctgtg agcacctgag actgcggtgg aatagcatcc agctcggaaa cacccgaaag
    28801 cagcgtactg tcatctgcca tcatcgtggc aaattgcggt agtgaagcgc gtttcgcgga
    28861 tttgttatag cgttcaacct cgacctcata atcaatcgtt tttgccgttg aggtaatggc
    28921 gagaatatcc agctttaccg gaatcggcgt cgcatcgcca tccaacaatt caaggcgcgg
    28981 cgcggcatca aaatggttag cttcccgctc attctccagg cgaggcaggc gaaaattgac
    29041 ctggttgatg agctgggtat taaaactgac caccagcggt ggggagatat acagccgttc
    29101 ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac actaactgat gcggtccatt
    29161 atccagttca atgctgtccg caccgcgcag cagagagctg gaaaccttct tgccgtcgag
    29221 taccagaaga tcgacatcgg ttgaaagccg cagcgtggtg gcaaaaacgg ataccggcaa
    29281 acataatgca atcaaggtgg tcacgatgcc ggttttcata ggtagctcct gtcaaaagac
    29341 cgcccgaata ataatgtgtc aaaatttttc tttaatcata cttactaaca tatagacata
    29401 tttcccgctt ttgctctcat tcattcgtat tagctgcatg gttggcatgt cggctttgtc
    29461 gtacactttg tgaaacagtc tggaggaaat aatgaaagaa accgatattg caggcatttt
    29521 aacgtccact cataccattg cgctggtggg ggcaagcgac aaacccgatc gtcccagcta
    29581 tcgcgtgatg aaatatctac tcgaccaggg gtatcacgtc attccggttt cgccaaaagt
    29641 tgccggcaaa acgctgctag gccagcaggg gtatggcacg ctggcagacg ttccggaaaa
    29701 ggtcgatatg gtcgatgttt tccgtaattc agaagcggcg tggggcgtgg cacaggaagc
    29761 tattgccatt ggcgcgaaaa ccctctggat gcaactgggg gtgattaatg agcaagcggc
    29821 agtactggca cgggatgccg ggttaaatgt agtgatggat cgctgcccgg ctattgagat
    29881 ccctcgcctg ggcctggcca aataaaaaat ccccggaagg caaaaacctt ccggggattt
    29941 gttcagggat tagttacgca gacgcggggc ctggagttgt ttgcggatgg tctgcgccag
    30001 ctcatccatg gatggctgtt caggatgttc atcctgcaat tcgctactta gctgggcttc
    30061 agccaggtag gtatgaaccg gtaggccgtt atcgtcctcc atcaccacgt gataccacgg
    30121 agcggcgcga agctcgtcat tcaccgccag ctcatcaggc gacggttcag agagcgaata
    30181 aaccgggtcg atatccacga ccactccgag ataacctaac agggaatggc ggacctgctg
    30241 gccgataccg aatttgctgg caatcatagt cacctcccgg gaaatcttta cctttgatat
    30301 aagggtaaaa ttccacattt caagttacat gacgcgacag gcaaaccctt tcagatatag
    30361 cccttccgga taggtagcga tcaccggatg atcggctgcc tgacggaact gctctataaa
    30421 ttgtacatca cggccggcat caattgcggc atccgcgatg attttctgaa ataaatcgct
    30481 ggtcatcaga ccggaacagg agaaagtcag gagaataccg ccttcattca gcaactgaat
    30541 cgccagcatg ttgatgtctt tatagccacg acacgcgccc atcaactggc ttttattctc
    30601 aacaaacttc ggcgggtcca tcacgataac gtcaaatttt tcaccgcgat cgcgataagt
    30661 acgcagcaat ttaaagacat catcacggac aaactcagcc ttgctcagat ccagtttgtt
    30721 cagctcaacg ttctgccgtg caatatccag ggcttcctgg gaggtatcaa cgctgacaac
    30781 ctggttgcaa ccgcccatca gtgccgatac ggcgaaacca ccggtatagg agaaacagtt
    30841 cagtacgcgt ttattttcaa cgtagcggcg ggtagccagg cggctgtcac gctggtccag
    30901 gtagtagccc gttttgtgtc cgtgctgaat atctaccagc agcttcatgc catgttcttc
    30961 aatcggcagc agggcaggcg gcaactcgcc ggtgacgagg ccctgggtca gctccatccc
    31021 ttctttttta cgtaccgcaa catcgctgcg atcgtaaatc gcacattccg ggtacagcgt
    31081 ttgcagggcg ctaattaatg ccgcgcgctg atactctgcc ccagcactca gcagttgcag
    31141 caccagaaaa ttaccgaaac gatcgatagt aatacccggc aggccatcag attctccggc
    31201 gattaaacga tagctgtcga ggccatcttt ttgcgccagc cagtcacgcc atttttgtgc
    31261 ttgttgcaaa cggcgggaaa aaaaagcaat gtcgatagac tcagacgggt caaacgtcca
    31321 gacgcgcgcc cggatttgcg aagctggcga ataagcgccg cgtgctaacc attttccctg
    31381 atgatcaaca atatcgatgg tttcaccgag gctggcttta ccttccatac gggcaacggc
    31441 cccggaaaag acccacggat ggcgacgaag taatgatttt tcgcgccctt tggctaacac
    31501 taaacgtaca ctcataattt acttttctgt cgatgcaaag aaatgggcgt cattgtccgg
    31561 agttttccat ggatttgcaa cgcgtccatt aaggataagg agagcagcaa tgtcgaaagt
    31621 ctgcataatt gcctgggttt acgggcgagt tcagggcgta ggatttcgct acaccacaca
    31681 gtacgaagcg aaaagactgg gcttaaccgg gtacgccaaa aatcttgacg atggcagcgt
    31741 ggaagtggtt gcctgcggtg aagaagggca ggtagaaaaa ttaatgcagt ggttaaagag
    31801 tggcggcccg cgttctgcgc gtgtagaacg ggtgctcagc gagccgcatc atccctcggg
    31861 ggaattaacc gattttcgta tccgttaaat acattttacc ggttttggca ggccagcaat
    31921 tttggtggct tgcttagccg gacctttcgg aaacagtcgg tataaatagc ggctgttacc
    31981 tttttcttcg ccaaatttat tcgccatcgc ttttaccagc atacgaatcg ccggagaagt
    32041 attgaattcc agatagaaat cacgcacaaa acgcaccact tcccagtgtt ctggcgacag
    32101 cgaaatccct tcgttctctg caatcaccac tgccagcggc tcactccact ggctgctttc
    32161 tttgagatag ccttcggtat ccgtttctat ctctttacct tcgaagatca gcataattat
    32221 tactacctta atcagactgc cggcagtgta acaaaaaaca aagccccgca taaagcgagg
    32281 ctatgaaagt gttagcgggt gagattaatc gcggctggcg aagcccagaa tgctcagcag
    32341 gctgacgaag atgttgtaca gcgaaacata caggctaacc gtggcacgaa tatagttcgt
    32401 ttcaccgcca tgaatgatgt tgctggtttc aaacaaaata gcgccagagg agatcaaaat
    32461 gaagaccgcg ctgatcgcca gatgcagagc aggcagttgc aggaagatat tagcaaccat
    32521 accaatcagc accaccacaa tacccgccat cagcataccg ccgaggaacg acatatcttt
    32581 gcgggtggtc agcacatatg cagagcaaca gaagaacact aacgccgttc cgcccagtgc
    32641 catagcgatt acgtcaccca ttccggcaga cagataggta ttcagaattg gtccgaggat
    32701 ataacccaga aaaccggtaa aggcgaatgc ggagataatc ccggtcggct tattcgccgt
    32761 tttataggtc aggaacatca aaccatacat acccaccagc gtcagaatca gacccggaga
    32821 tggcagcatc agcaccgtac tggcagttgc ggtaatcgcc gaaaaggcca gcgtcaggct
    32881 cagcagaaaa taggtattac gcagcacctt atgggtgcta agcagtgatg tacggtcatg
    32941 tgaagaacta acaatacgat ccattgagtc actctctatg acagatgtaa ttaattaagc
    33001 agcataatga taatgcgtaa gggcacccag aagttttacc catctttacg catttgatct
    33061 ggaacaggtt taacagcgga ttatcaggtc attaagcaaa tataacgccc tgagaatttc
    33121 gacaggcaaa agaaaaaggg gttagcattt agctaacccc ttatcttatc tggcggaagc
    33181 gcagagattc gaactctgga accctttcgg gtcgccggtt ttcaagaccg gtgccttcaa
    33241 ccgctcggcc acacttccgg aatgacgcgc actataaaca tcccgatgcg gcgtgtaaac
    33301 ccctaatttg tttgtttgcc tgaaaaacag ccaaaagtgc attgatagcg tgaaataaaa
    33361 gcagattgat catttcatca ccataaattc cttctctttt actcgtttag caactggcta
    33421 aacatcccac cgcccggcaa aaagaaaaat aggtccattt ttatcgctaa aagataaatc
    33481 cacacagttt gtattgtttt gtgcaaaagt ttcactacgc tttattaaca atactttctg
    33541 gcgacgtgcg ccagtgcaga aggatgagct ttcgttttca gcatctcacg tgaagcgatg
    33601 gtttgccttg ctacagggac gtcgcttgcc gaccataagc gcccggtgtc ctgccggtgt
    33661 cgcaaggaga agagacgtgc gatatgaata acgaggaaac attttaccag gccatgcggc
    33721 gtcagggcgt tacccggcgc agctttctca aatattgtag tctggctgcc acgtcgctgg
    33781 gattaggcgc gggaatggca ccaaagattg cctgggcgct ggagaacaaa ccgcgcattc
    33841 cggtagtatg gatccacggt ctggaatgca cctgctgtac cgaatctttt atccgctccg
    33901 ctcacccgct ggcaaaggac gtcatccttt ccctgatttc cctcgattac gacgatactt
    33961 tgatggctgc cgccggaacc caggcggaag aagtatttga agacatcatc acgcaataca
    34021 atggcaaata tatcctcgca gtagaaggta atccgccgct gggcgagcag gggatgttct
    34081 gtatcagcag cggtcgaccg tttattgaga aactcaaacg tgccgctgcc ggagccagcg
    34141 cgattatcgc ctggggaacc tgcgcgtcct ggggctgcgt gcaggccgcg cgacctaatc
    34201 cgacacaggc aacgcctatc gacaaagtca tcaccgacaa acccattatc aaagtacctg
    34261 gctgcccgcc gatcccggat gtgatgagcg ccatcattac ttacatggtg acctttgatc
    34321 gcttgccaga tgtcgacaga atgggtcgtc cactgatgtt ttatggtcag cgtatccacg
    34381 ataaatgcta tcgccgcgct cacttcgacg ccggagagtt cgtccagagt tgggatgatg
    34441 acgctgcccg caaaggttat tgcctgtaca aaatgggctg caaagggcct accacctata
    34501 acgcctgttc ctccacacgc tggaatgatg gcgtttcttt cccaatccag tctggtcacg
    34561 gctgcctggg ctgtgcggaa aatggtttct gggatcgcgg ttcgttctac agccgcgtgg
    34621 tcgatattcc gcaaatgggt actcattcca ccgccgatac cgtcggttta accgcgcttg
    34681 gcgtggtggc agcggctgtt ggtgtgcacg cagtcgccag cgccgttgac cagcgcagac
    34741 gtcataacca gcaacctaca gaaaccgaac atcagccagg caatgaggat aaacaggcat
    34801 gagcactcag tacgaaactc agggatacac catcaataat gccggacgcc gcctggtggt
    34861 cgacccgatt acgcgcattg aaggccacat gcgctgcgaa gtgaatatta acgatcagaa
    34921 tgtgatcacc aatgccgtct cctgcggcac catgtttcgc gggctggaga tcatcctgca
    34981 agggcgcgac ccgcgcgatg cgtgggcgtt cgttgaacgt atctgcggcg tctgtactgg
    35041 cgtacacgcc ctggcttcgg tttacgccat cgaagatgca atcggtatta aagtgccgga
    35101 caacgccaat atcatccgca acattatgct ggcaacgctc tggtgccacg atcatctggt
    35161 gcacttctat cagctcgccg ggatggactg gatcgatgtg ttagatgcgc tgaaagccga
    35221 cccgcggaaa acctccgaac tggcgcaaag tctctcctca tggccgaaat catcccctgg
    35281 ctatttcttc gacgtacaaa atcgcctgaa gaaattcgtt gaaggcgggc agttggggat
    35341 cttccgcaat ggctactggg ggcacccgca gtacaaactg ccgccagaag ccaacctgat
    35401 gggctttgcc cactatctcg aagctctcga tttccagcgt gaaattgtca aaatccacgc
    35461 ggtctttggc ggtaaaaacc cgcatccaaa ctggattgtc ggcgggatgc catgcgccat
    35521 taacattgac gaaagcggcg cggtcggggc ggtcaatatg gaacgcctga acctggtgca
    35581 gtcgattatc acccgcacgg cagacttcat taacaacgtg atgatccccg acgccttagc
    35641 catcggtcag ttcaacaaac cgtggagcga aatcggcacg gggctttctg ataaatgtgt
    35701 cctcagctac ggcgcattcc cggatattgc caacgacttt ggtgagaaaa gtctgctgat
    35761 gcctggcggc gcggtgatta acggcgactt caacaatgtg ctgccagtgg atttggttga
    35821 tccgcagcag gtgcaggagt ttgtcgatca cgcctggtat cgttatccca acgatcaggt
    35881 cgggcgtcat ccattcgatg gcattaccga cccgtggtac aaccccggcg atgtcaaagg
    35941 cagcgatacc aacattcagc agctgaatga acaggaacgc tactcgtgga tcaaagcgcc
    36001 gcgctggcgc ggtaacgcga tggaagtggg gccgctggca cgcacgttaa tcgcttatca
    36061 caaaggcgat gctgcgaccg ttgagtcggt cgatcgcatg atgtcggcgc tgaacctgcc
    36121 gctttccggt atccagtcaa cgttgggccg cattttgtgc cgcgcgcacg aagcacagtg
    36181 ggccgcaggt aagttgcagt atttcttcaa caagctgatg actaacctga aaaacggcaa
    36241 tcttgccacc gcctccacgg aaaaatggga acccacaacc tggccgacag agtgccgtgg
    36301 tgtcggcttt accgaagcgc cgcgcggggc gttaggccac tgggccgcca ttcgcgatgg
    36361 caagattgat ctctaccagt gcgtggtgcc gaccacctgg aacgccagcc cgcgcgatcc
    36421 caaagggcag attggcgctt atgaagcggc gctgatgaac accaaaatgg cgatccccga
    36481 gcaaccgctg gagatcctgc gtactctgca cagctttgat ccgtgcctcg cctgttcaac
    36541 acacgtgctg ggcgacgacg gtagcgagct gatctccgtg caggtgcgtt aacagcgaag
    36601 gagaatcatc atgcaacaga aaagcgacaa cgttgtcagc cactatgtct ttgaagcgcc
    36661 agtgcgtatc tggcactggc tgacggtgtt atgcatggcg gtgttgatgg tcaccggtta
    36721 ctttatcggc aaaccgctac cttccgtcag cggcgaggcg acgtatctgt tctatatggg
    36781 ctacatcagg ttaattcact tcagcgccgg gatggttttt accgtggtct tgctgatgcg
    36841 gatctactgg gcttttgttg gcaatcgata ctcccgcgag ctgtttatcg tgccagtatg
    36901 gcgtaaaagc tggtggcagg gcgtgtggta tgaaatccgc tggtatctgt ttctggcaaa
    36961 acgtccgagt gccgatatag gccataaccc catcgcccag gcggcgatgt ttggctattt
    37021 cctgatgtcg gtctttatga tcatcaccgg ctttgcgctg tacagcgaac acagccagta
    37081 cgctattttt gcgccgttcc gttatgtggt ggaatttttc tactggacgg gaggcaactc
    37141 aatggacatt cacagctggc atcggctggg gatgtggctg attggcgcgt ttgtgatcgg
    37201 tcatgtctat atggcgctgc gtgaagacat catgtccgac gacacggtga tctccaccat
    37261 ggtcaacggc taccgtagcc acaaatttgg caaaataagt aacaaggagc gttcatgagc
    37321 gagcaacgcg tggtggtcat ggggctgggc aacctgctgt gggccgatga aggtttcggc
    37381 gtgcgggtgg cggaacggtt gtatgcccat taccactggc ccgagtatgt ggagattgtc
    37441 gatggcggta ctcagggact gaacttgctg gggtatgtcg aaagcgccag ccatctgttg
    37501 attctcgatg ccattgacta cgggctggaa cctggaacgc tgcgaaccta tgccggagaa
    37561 cgtattccgg cttatctcag cgcgaagaaa atgagcctgc accagaacag tttctccgaa
    37621 gtgctggcgc tggcagatat ccgcggacat ctgccagcac atattgccct cgtcggtctg
    37681 caacccgcaa tgctcgacga ctacggcggc agcctgagcg aactggcacg ggagcaactg
    37741 cccgcagcgg aacaggcggc gctggcgcag ctgggtgcat ggggaattgt gccgcaaccg
    37801 gctaatgaat cgcgctgtct caattatgac tgtctgtcga tggaaaatta cgaaggcgtt
    37861 cgcttgcgcc agtacaggat gacactggag gagcagggat gagcaacgac acgccatttg
    37921 atgcgttgtg gcaacgaatg ctggcgcgcg gctggacgcc ggttagtgaa ttccgtcttg
    37981 acgactggct tacgcaagcg ccagacggcg tggtgttatt aagcagtgac ccgaaacgca
    38041 cgccagaggt cagcgataat ccggtaatga ttggcgaatt actgcgcgag tttcccgact
    38101 acacatggca ggtggcgatt gctgaccttg aacagagcga agccatcgga gatcgctttg
    38161 gcgtctttcg ctttcccgcc acattagtgt ttaccggcgg aaactatcgc ggcgtgctga
    38221 atggtattca cccgtgggcg gaactgataa acctgatgcg tgggcttgtc gaaccacaac
    38281 aggagcgtgc ctcatgagcg aaactttttt ccatctgctg gggccaggaa cgcaaccgaa
    38341 cgatgacagt ttcagcatga atccgctgcc gatcacctgt caggtgaatg atgaaccgag
    38401 tatggcggcc ctggaacagt gtgctcacag cccgcaggtg attgcgctgt taaacgagtt
    38461 acaacatcaa ctaagcgaac gccagccgcc gttgggcgag gtgctggcag tcgatctgtt
    38521 aaatctcaat gccgacgatc gtcactttat caatacgctt ctcggggaag gggaagtgtc
    38581 agtgcgcatt cagcaggctg acgacagcga aagtgaaata caggaggcga tcttctgcgg
    38641 attatggcgg gtgcgcagac gtcgcggcga aaagttgctg gaggacaaac tggaagctgg
    38701 ctgcgcgccg ctggcgttgt ggcaggcggc aacgcaaaat ctcttgccga cagattcgct
    38761 gttaccgccg cccattgatg gcctgatgaa tggcctaccg ttggcgcatg agttactggc
    38821 gcatgtacgt aaccccgacg cgcagccgca cagcattaat ctgacgcaat tacccatcag
    38881 cgaggctgat cggctttttc tctcacgtct ctgtgggccg ggaaatattc agattcgtac
    38941 cattggctat ggcgagagct atatcaacgc cacggggtta cgccatgtct ggcatttacg
    39001 ctgtacggac accttaaaag gcccgttact ggaaagttat gaaatctgcc caataccgga
    39061 agtggtgctg gcagcgccag aagatttggt cgactctgcg cagcggctta gcgaggtatg
    39121 tcagtggctg gcggaaggtg caccgacgta aaaagacggt aagtatcgct ttcagtctta
    39181 tgaatatcgc aatcggcgaa tacctctggt cgtagagttt caggataaag agggagatct
    39241 accattatcg ggttattttt ctctcttcgc ctacaggagt gcgcatgtgg gatgtcattg
    39301 atttatcgcg ctggcagttt gctctgaccg cgctgtatca ctttttattt gtacccctta
    39361 ccctggggct gatttttttg ctggctatca tggaaaccat ttacgtggtc accggcaaaa
    39421 caatctaccg cgatatgacg cgcttctggg gtaagctctt cggtatcaac tttgctcttg
    39481 gcgtggctac cggcctgacc atggagtttc agtttggtac taactggtca ttctattcca
    39541 actatgtggg cgatattttt ggcgcaccgc tggcgatgga agcattaatg gccttcttcc
    39601 tcgaatccac ctttgtcggg cttttcttct tcggctggca gcggctgaat aaataccagc
    39661 acctgctggt gacgtggctg gtggcgttcg gttcaaatct ctctgcattg tggatattga
    39721 atgccaacgg ttggatgcaa tacccgaccg gtgcgcattt tgatatcgac accctgcgta
    39781 tggagatgac cagcttcagc gagctggtct ttaacccggt cagccaggtg aaatttgtgc
    39841 acaccgtaat ggcgggctac gtgaccgggg ccatgtttat tatggcgatc agcgcctggt
    39901 atttactgcg cggacgggag cgcgatgtcg cattacgctc gtttgccatc ggttccgtct
    39961 tcggtactct ggcgattatt ggcacgctgc aactcggaga cagttctgcg tatgaagtcg
    40021 cgcaagtaca accggtaaaa ctggcggcga tggaagggga gtggcaaacg gaacctgcac
    40081 ctgcaccgtt ccatgtggtt gcatggccgg aacaggatca agagcgtaac gcctttgcca
    40141 tcaaaattcc cgcgctgcta gggatcctcg ccactcactc attagataaa cccgtgccgg
    40201 gtctgaagaa tttgatggct gaaacctacc cacgcttgca acgcggacgt atggcctggc
    40261 tgttaatgca ggaaatatcg caaggcaatc gtgagccgca tgtgttgcag tcattccggg
    40321 agctggaagg cgacctgggc tacggcatgt tgctctcccg ctatgcgccg gatatgaatc
    40381 atgtcacagc cgcacagtac caggcggcga tgcgtggcgc gatacctcag gttgcgccgg
    40441 tattctggag tttccgcatc atggtgggct gtggttccct gctgctactg gtgatgctta
    40501 ttgcgcttgt ccagacgctg cgtggcaaaa tcgaccagca tcgctgggtg ctgaaaatgg
    40561 cgctctggag tttgccgctg ccgtggattg cgattgaagc cgggtggttt atgaccgagt
    40621 ttggtcggca gccgtgggcg atacaggaca tcttaccgac atactccgcg cactccgctt
    40681 taaccacagg acaactggct ttctcactga tcatgatcgt aggcctttac accctgttct
    40741 taatcgccga agtctacctg atgcagaaat atgcccgtct ggggccgagc gcgatgcaga
    40801 gtgaacaacc gacgcagcaa caggggtaaa ggagaaaatc atgtttgatt atgaaacatt
    40861 gcgcttcatc tggtggctgc tgattggcgt gatcctggtg gcctttatga tctccgacgg
    40921 atttgacatg gggattggct gtttgctgcc gctggtggcg cgtgatgatg atgaacgccg
    40981 gatagtgatt aatagcgttg gtgcgcactg ggaaggcaat caggtctggt tgatcctcgc
    41041 aggtggggca ttatttgccg cctggcccag agtgtatgca gcggcgtttt ccggctttta
    41101 tgtggcgatg atcctggtgc tgtgctcgct gttcttccgc ccgctggcct ttgattatcg
    41161 cggaaaaatc gccgatgcac gctggcgtaa aatgtgggat gccggtctgg tcatcggcag
    41221 tctggtgccg ccggtagtct tcggtatcgc cttcggcaac ttgttgctcg gcgtgccgtt
    41281 tgccttcact ccgcaattac gcgtggagta tctcggcagc ttctggcaac tgctgacgcc
    41341 attcccttta ttgtgcggat tgctcagcct tgggatggtg attttgcaag gtggcgtctg
    41401 gttacaactg aaaactgttg gtgtgattca tctgcgttca cagctggcga ctaaacgcgc
    41461 tgcactgttg gtgatgctgt gctttttgct ggcgggttac tggctgtggg tcggtattga
    41521 tggctttgta ctgctcgccc aggatgctaa cggtccttcc aatccgttaa tgaaactggt
    41581 ggcagtgcta cctggtgcct ggatgaataa ttttgtcgag tcgcccgttt tgtggatctt
    41641 cccgctgctg gggttcttct gcccattgct gacggtgatg gcgatttatc gtggtcgccc
    41701 gggttgggga tttttgatgg cgtcattgat gcaatttggc gtgattttca cggcaggcat
    41761 cacgctgttc ccctttgtca tgccgtcaag cgtgagtcca atctccagcc tgacgttgtg
    41821 ggacagtact tccagtcagc tgacgctgag cattatgttg gtaatcgtgc tgatattttt
    41881 gcccattgtg ttgctctaca ctctctggag ctactacaaa atgtggggac gcatgacgac
    41941 agaaactctc cgccgtaacg aaaacgagct gtactaagga gcaggaacaa tgtggtattt
    42001 actttggttc gtcggcattt tgttgatgtg ttcgctctcc acccttgtgt tggtatggct
    42061 ggacccgcga ttgaaaagtt aacgaacgta agtctgatgc ggcgcattag cgtcgtatca
    42121 ggcaatcaat aatgtcggat atgaaaagcg gaaacatatc gatgaaagcg atcttgatcc
    42181 catttttatc tcttctgatt ccgttaaccc cgcaatctgc attcgctcag agtgagccgg
    42241 agccggagct gaagctggaa agtgtggtga ttgtcagtcg tcatggtgtg cgtgccccaa
    42301 ccaaggccac gcaactgatg caggatgtca ccccagacgc atggccaacc tggccggtaa
    42361 aactgggttg gctgacaccg cgcggtggtg agctaatcgc ctatctcgga cattaccaac
    42421 gccagcgtct ggtagccgac ggattgctgg cgaaaaaggg ctgcccgcag tctggtcagg
    42481 tcgcgattat tgctgatgtc gacgagcgta cccgtaaaac aggcgaagcc ttcgccgccg
    42541 ggctggcacc tgactgtgca ataaccgtac atacccaggc agatacgtcc agtcccgatc
    42601 cgttatttaa tcctctaaaa actggcgttt gccaactgga taacgcgaac gtgactgacg
    42661 cgatcctcag cagggcagga gggtcaattg ctgactttac cgggcatcgg caaacggcgt
    42721 ttcgcgaact ggaacgggtg cttaatttcc cgcaatcaaa cttgtgcctt aatcgtgaga
    42781 aacaggacga aagctgttca ttaacgcagg cattaccatc ggaactcaag gtgagcgccg
    42841 acaatgtctc attaaccggt gcggtaagcc tcgcatcaat gctgacggaa atatttctcc
    42901 tgcaacaagc acagggaatg ccggagccgg ggtggggaag gatcactgat tcacaccagt
    42961 ggaacacgtt gctaagtttg cataacgcgc aattttattt actacaacgc acgccagagg
    43021 ttgcccgcag ccgcgccacc ccgttgttgg atttgatcat gacagcgttg acgccccatc
    43081 caccgcaaaa acaggcgtat ggtgtgacat tacccacttc agtgctgttt atcgccggac
    43141 acgatactaa tctggcaaat ctcggcggcg cactggagct caactggacg cttccaggtc
    43201 agccggataa cacgccgcca ggtggtgaac tggtgtttga acgctggcgt cggctaagcg
    43261 ataacagcca gtggattcag gtttcgctgg tcttccagac tttacagcag atgcgtgata
    43321 aaacgccgct gtcattaaat acgccgcccg gagaggtgaa actgaccctg gcaggatgtg
    43381 aagagcgaaa tgcgcagggc atgtgttcgt tggccggttt tacgcaaatc gtgaatgaag
    43441 cacgcatacc ggcgtgcagt ttgtaatgta taaaaaagag cattcagcta cctgaatgct
    43501 ctgaggctga tgacaaacga agaactgtct aatgcgtaga ccggaaaagg cgttcacgcc
    43561 gcatccggtc actttacctt cagttttact ctttctcgga gtaactataa ccgtaatagt
    43621 tatagccgta actgtaagcg gtgctggcgc gtttaatcac accattgagg atagcgcctt
    43681 taatattgac gcctgcctgt tccagacgct gcattgacaa actcacctct ttggcggtgt
    43741 tcaggccaaa acgtgcaacc agcaggctgg tgccgacaga gcgccccacg accgcagcat
    43801 cgctcaccgc cagcatcggc ggcgtatcga caatcaccag atcgtaatgg tcgttcgccc
    43861 attccagtaa ttgacgcatc cgatcgcgca tcagcagttc agacgggtta ggtggcacct
    43921 gaccgcgagt aatcacatca aagcctcctt tgccaaaatg ctggatcact ttgttgagct
    43981 catctttacc tgccagatat tccgacaagc catgttcatt actcacggta aacaggttat
    44041 gcgaataacc acggcgtaag tcggcatcaa taaataacac tttttgatcg gactgggcga
    44101 tcaccgctgc cagagttgaa ctgacaaatg ttttaccact gtctggcgtc gcaccggtga
    44161 tcatcagaat gttattctca gtctccatca tagcgaaatg cagactggtt cgtagcgcac
    44221 gtacggcttc cacagcagaa tccgccgggt tatccaccgc caggaagggg atatttttag
    44281 tacgatggcg ctgctgatta gaaaataaat ttttcttacg cagacgggtg cgtttatcca
    44341 gccactcgga cattgggata gtggcataaa cgctgatgcc gtgctcttcc agttgttccg
    44401 gtgcttctac accacgacgc aacatcgcac gcgccagcac ggcacccacc gaaataaaca
    44461 ggccaagaat aaaaccaagc accacgttca acgctttttt cggtttcact ggctgcggct
    44521 gagtgactgc cgggtcgata atccgcacgt taccaatggc actggatttc gaaatactca
    44581 actcctgctg gcggttaagt aattgcagat ataccgcacg gcccgcttct acgtcacgac
    44641 ttaaacgcaa cacttcctgt tgggtggaag gcatagccga tacccgctta ttcaggcgtt
    44701 tgcgttcttg ctccagcgtc tggcgttttt ccagcagcgc acgataagtt gggtgatctt
    44761 tcttatacag ctgggagatc tctgcctcgc ggaaggtcag ctcattgagt tgattatcaa
    44821 cgttcacaat ctgctcaaga acggctttgg cttccaggtt aaggtcaacc gaatcgcgct
    44881 gctggcgata aacgttgagt ttttcttccg cctggtccag ctcgctgcgc acttcaggca
    44941 actggcgctg taagaattca aggctttgtg aatcctgcgc cgcctggcga gcgatattct
    45001 gttgcaaata gttgttagcg atgctgttca gaatacgggt aatcaactgg ggatcatcac
    45061 cggtcatggt aagttccagc atcccgcttt ctttactgcg ttcgctaacg gtaaaggttt
    45121 cctgcaacgc gttaatcgct tccagttcgg tacgttggct caggacaaac tgtgttcctg
    45181 gtttggcctt aatatccgct atagtcagcg caacgccatc tttttccaga cgctggccga
    45241 ccataccatt gacggtgaac tcttcacctt ccagcgtata gtggccgttt tccccaaccg
    45301 tgagtgtcag ttgctgatcc tgaccattca gttgtggaat atgcatccag ctgatcgcca
    45361 gctcacctgg tttctctttg gttaatctcg cccagccgcg acccacaatc ggaaaatact
    45421 tctgctcaac catgtcgcgc agattcagtt cagcaatggt tttaccgaga atcatgcgcg
    45481 attgcagcag ttggatctcc ggtgcagact cgggcgatga gttagggatc atatcactca
    45541 ggccgctgag aatggcgttg ccctgttttt gctcaacctg gaccagagta tctgcctgat
    45601 aaattggtgt gcttaacagc gagtaagcga cagcgatcag cgtgaataac gcggtcacgc
    45661 tgataataaa cttacggtga tcccataact cgccgaccag acgaagcaga tcgatctcat
    45721 tttcctgagt gctgcctggt ggcgtattca tatttttagt tgtcatcgta ttttataaac
    45781 tcaaattacc ggctgaggcg cttcgcccat tcctgactgg cgcgctccaa cataccgtag
    45841 acatgttcaa atgcgtcctg acttttacga tagggatccg ggatctcttt ctgttccagc
    45901 cattgcccaa acagcattgt cttcccgcgg acctcgggag cgattgcggt aacctgagca
    45961 atatgttccg actccattgc cagaatcaga tcgtaatttc gtgccatctc agcagtgagc
    46021 ttacgtccgg catgtccttc aagagatacg ccatgattag cggcaacatc tgccgctgtc
    46081 gcatctgcag ggtgttttac cagaccatga acacctgccg atttgacttt tacgcccggc
    46141 agacgtttac gcagcaagcg ctcgccaatg ggcgaacggc aaatgttgcc ggtacaaacc
    46201 accaggattg agttaaattt tagttgggcc atgtttgaat ccaccgtgaa gtctctgtca
    46261 ggtcatgcac cccggaaatc gtcggtacca gcagagaaat cacacggttc caacgagcca
    46321 gaggcgcggt agtgacataa acaatgtcgt aaggttcaag ctggaattcc gttccgagta
    46381 tcattgccga tgcgtccttc gcattcagct gataaatatt ggcgattttg ccattctgct
    46441 tgttttgcgt tgcacgaata acaaaaatcc ccgtcgcatc agcgacatcc tgattcatac
    46501 cctcagcgtt acccagcgct tccgccaggg tcataccgct gcggtccatt ttcagcgtgc
    46561 tctgtttacc cacttcaccc atcacgaaga ctttcagatc atcgttgcgc ggaataaata
    46621 aaatatctcc cgggtgcagc agtttgttct ggcgcaagtc gccacgctgc atcagcgcat
    46681 agaggttcac tttggtcttt acgccattct gggtgagcac cacattgcgc cagtctgcat
    46741 ctgcggtcag accgcctgcg gcgttaatcg catccatgat ggtcagagga atattggtaa
    46801 tcggctgctg acccgatttg ctgacttcgc cggtgacgta cgctttttgc gatcggaacg
    46861 ccgcgacgct gacatcgacc tgcgggcttt caatgactga atccagacgc gctgtaatct
    46921 cgttgcgtac ctgggtaagt gttttcccgg ccactttcag gcgaccaata taggggtaga
    46981 aaatcgcacc atcggcgtta acccagttgc cggtatcact ggcgctacgg tattgccctg
    47041 cgggggtggt tagttccggg tgatcccaga ccgtaaccat caacacatca ccaatgccga
    47101 tacgatattc atagttcgcg atttgctgct ccagctccgg gttagcctgc gacataatcg
    47161 gtttggcgcg caactgatca atcaatcctg gcgtgacggg atagatattg accattttgt
    47221 ccaggtcata ctgattgtca ggtagttcaa tgacatcctt gttactggtc gataaattct
    47281 gaccaggaac aagggtacat gctgtcaaag acagaacagc caatgtcagt accgaaaact
    47341 taaaaatgtt ctttttcatt ggtttatatt tatcagtcaa tgtaatgtta tgaattacgt
    47401 caggttattg tccaaccggt acttctcgct ctgccgtcat tggatagagc tggtatttgc
    47461 gtgacagcat ttgtccacca tcacgcgtca atggtgtcca cgaaaccacc gcgcggttgc
    47521 ggttaggtcc aatggtcatc aagtcgaacg gaatcgagat ataaaaacct ttactaaagc
    47581 cgccttcgcc gtagtcatct ttagatacgt tactgattgc cgcccatacc cctaccgcca
    47641 cgccgctgtc aaagcgtttg gcgacgtcga tcgttgcgcc tttatctttt gccagatatt
    47701 gcccaacgct aagtttcatc agtacgccat tgagcgtcgg cgggttccag taagccgtca
    47761 cgaaacctgt gggcgtggaa taatcggtga agcgcatcat gttgtcccag tcacgttgct
    47821 tcacgtagtt aacgtccaca cccagcgccc agctggcatc tagcgggcga taaagcagct
    47881 cggaaccgac accggcgtac atcgtttcca gataaccgcc atacacctgg ccatagaaac
    47941 cattgcctaa gtcggcaaag tagttcgcct gcaagttgtt gagataaacg tcattgcgaa
    48001 cgtagtcacg gatatgcgtg cgcacgcggg gcagggtaga gtccgcgggc aacagtgaag
    48061 acttaaactt gtcgtagttg ttgtaaatat tggtgaaaat accgccatca agcagcaggt
    48121 ggtcggtaaa ccagtagcgg gcgctggaca tcagccccag ctggaacata tagaaatctt
    48181 ccgggccgcc cagcgactgt gaaagtgttg ggttgaaaga gtagctaaag cgatcttcac
    48241 gaatacggta gccccgacca aaggcagaaa gatcttcagc ttcaacacgt tgttgttgca
    48301 gtggctctga ttgaccaggc gctgtacctg ccagctgttt gcgcaggctg gctacgtcgg
    48361 tttcggtagt caccatcgcc atatgctcgc gcttttgcgt cacgctaatc ttctcaacgc
    48421 cttgcggcag gttattcacc agaatccggt tggcacgatc gaccgcttca cgagagtcac
    48481 ggtatttgta ttgctgacca gacatataca gcgtcttatc gcgtagctga atttctggcg
    48541 cgtcaaagcc cgcgttatac ttcagggcgg taagttggtt tgctaccgtg gtgtactgca
    48601 atccttcaga ttcaggcgca ggttgatatg ccggttttgg cgtatcgcgc agggcaggac
    48661 gcagatcgtt gaaattggtc cgtaaagtga aaccaaacat caacgtgtta ccgcgttcat
    48721 aactcaggtt gaggtctgcc cagctggcag cgcgataaac tgcaccgacg ttgaaatggc
    48781 ttgcctgagg cagtttgcca gcgaaatcat tctggtaatt gttgccgtcg tattcgagtt
    48841 tcagacgcag gggattccac ggcgtttgat actcaatgcc gccaaagatg gaagccgggc
    48901 cacgaaagat atcgctaaag ctgatatcgc ccgcatcgtg agactctgcg cgatgacaat
    48961 atttatcgct cacccggcaa aacgggttgg taatattgcc cgcattaccg gcgtagcccc
    49021 atgccatccc gagggtgaaa tcaaatggcc ccgcttgctt gctggccacc aaatactcac
    49081 cgtcaaacag acccgtacca gcaatatcac gtttaccaaa cgccacttgc ggtagccagt
    49141 aaccttcttc ccacaggcga agtttaaaat cgaatgattt gtctttgtat gactgatcgc
    49201 cgctgaaatc ttcccactgg ctatatttac gtgtgcgcac atccgtataa cgaatggtgc
    49261 cttccagcca tgggaacagc gccacggagg tggagtagaa acggtattga tcgttatccc
    49321 ggtagttgac gctgaattca ccttccggtg cgatgcgcgc atttggcatc tgcaacaatc
    49381 ctgtgccgcc aaagtccgat tgcgacgaac ccagcggatc cgggtaggtt aatacttcag
    49441 catgacaggc actggagacg gcaatggcca ggcagcttaa aagataagaa ttcttcttca
    49501 tcagtcagga acacgttgcg ttaaaacaga cacaatctgg tcatttaaat cagcatattt
    49561 ttcgggcagt acatgcgcag aaaagcctaa ccaaagctgg ctgccaggcg gtggttccac
    49621 atgtctttta ttccacaggg caacaggggc gacgacagtc tcaccctcag gcgtaatcac
    49681 gataacgtta ttcttgtccg cgcctgcaag acgggggtga tcttgcagat aatcggtaac
    49741 gctacggcct gcctgccagg gcaattgccc agccccagaa accgcaccca gcagagtaat
    49801 agtcaccgga cgctgtacgg tatagagcgt atagtccccg accagcgggg gattgctgtt
    49861 ctcatccact cgtacaaaat cagggtccag tttgaccgct aagcgaccag tgatattgag
    49921 attgagtaac tgctggcgca cggatttaat cgttgccgcc acatcgtcgt cagcttctgc
    49981 ttcccaggaa gcaagctgtg ccatgacatg ttggtagtct ttaagcgctt tagctttcgc
    50041 cgcagagtcg gtcagcaagg caccaggcca ccagaggcga tcgcgcagtt gcggttgtgt
    50101 caccaactgg gcaacatttt ccacggggcc aacggaaagt gtctgttgtt cgccaggcag
    50161 ataaatagtc accgttcctt gcgcaaaggc atggggtgtc attacgtaaa gtacgctggc
    50221 aatgaaatac gactgtaatt tattcatggt gcgggtttaa gaaacgtcat ttctacggga
    50281 ataacccctg cgcctaacat ttgccgactt tgacgaacct ggccagtagc cgaatcgacc
    50341 cagaatgtgt tttgccactg tttttctggc agcgtggaag tcacctcttc ctgccagaca
    50401 ttacacagca catcgcgacc cgcaatatgc agcgtctcta atccagcaaa ggaaaaagtg
    50461 gaactcaggg tcgcagagcg aaaatggttg tcttcactcc agagaatatc gcgcgtccag
    50521 cggctgccgt cttttattgc cagcgcgtta cgcagagggt cctgcccgga atttgtcact
    50581 tccagcagat tattattaag tttgacggtc tttaaaagac gtccattgtg ggtaaccagc
    50641 atggcgttat cctgggataa ccatttgctt tgttcttgtt ctatatatcc cagtaccaca
    50701 aagattcgtt gcccttcatt aaggcgtaaa tacatcgtgg aataaggcaa cgcctggatc
    50761 tgctgatccg ctacggtgat atcctgatta tcgaaaaggc tggcgcgaaa tgtatcgacc
    50821 atactttgct ggctgtgcgt acatcccgca agaaatagtg cgaaaatcga taaaataaga
    50881 gggcgcacct ttaatccttg aaaggttaat taaaaaaatg caaatctcta aagcccggta
    50941 cgaatatcgc aaccgggtat tatttaactt taatttcgac gcgtttaagc agaattagcg
    51001 cgtagtggac gtggtggttg aggtgttatg agaaccgcca tcgccactgt catttgctgc
    51061 aactactgcc aacgtcgcca caccaacagc cgttgccaca ccgacaactg tcgccgtgcc
    51121 gacagcgcca gcagccacgc caccagtggt agcagccgtc gtggttgcac cggtagtggt
    51181 cgttgtggtg ccagtagtac ccgtcgttgc ctcggtcccg tttgctgctt caggggcagc
    51241 aaacgcagct ggcgtcgtca acatgaaggc cataaggatt gcagaaagct tgtgtttcat
    51301 aacttttcct ttattcatcg catggacaat acgtacgctt gagatcagga ttgtctactt
    51361 tgagtataac atccttgtga aatctccctt tcctcatacc acttgcacta tatacagcgg
    51421 gtgagaaagg cgcgcagagg gaaatataag attgttaact
//
LOCUS       DAZKFU020000004        49832 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZKFU020000004 DAZKFU020000000
VERSION     DAZKFU020000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 49832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.004"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            136..471
                     /gene="eutS"
                     /locus_tag="ACHOQQ_RS00810"
                     /old_locus_tag="pgaptmp_000162"
     CDS             136..471
                     /gene="eutS"
                     /locus_tag="ACHOQQ_RS00810"
                     /old_locus_tag="pgaptmp_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGU4468477.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            484..963
                     /gene="eutP"
                     /locus_tag="ACHOQQ_RS00815"
                     /old_locus_tag="pgaptmp_000163"
     CDS             484..963
                     /gene="eutP"
                     /locus_tag="ACHOQQ_RS00815"
                     /old_locus_tag="pgaptmp_000163"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGU4468478.1"
                     /translation="MKRIAFVGTVGAGKTTLFNVLQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            938..1639
                     /gene="eutQ"
                     /locus_tag="ACHOQQ_RS00820"
                     /old_locus_tag="pgaptmp_000164"
     CDS             938..1639
                     /gene="eutQ"
                     /locus_tag="ACHOQQ_RS00820"
                     /old_locus_tag="pgaptmp_000164"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGU4468479.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESSPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWDNAFFPWTLNYDEIDMVLEGELHVRHAGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1636..2439
                     /gene="eutT"
                     /locus_tag="ACHOQQ_RS00825"
                     /old_locus_tag="pgaptmp_000165"
     CDS             1636..2439
                     /gene="eutT"
                     /locus_tag="ACHOQQ_RS00825"
                     /old_locus_tag="pgaptmp_000165"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGU4468480.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLGHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2436..3452
                     /gene="pta"
                     /locus_tag="ACHOQQ_RS00830"
                     /old_locus_tag="pgaptmp_000166"
     CDS             2436..3452
                     /gene="pta"
                     /locus_tag="ACHOQQ_RS00830"
                     /old_locus_tag="pgaptmp_000166"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4468481.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTE
                     PLMFAAAMVSAGIADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3491..3784
                     /gene="eutM"
                     /locus_tag="ACHOQQ_RS00835"
                     /old_locus_tag="pgaptmp_000167"
     CDS             3491..3784
                     /gene="eutM"
                     /locus_tag="ACHOQQ_RS00835"
                     /old_locus_tag="pgaptmp_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGU4468482.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3891..4178
                     /gene="eutN"
                     /locus_tag="ACHOQQ_RS00840"
                     /old_locus_tag="pgaptmp_000168"
     CDS             3891..4178
                     /gene="eutN"
                     /locus_tag="ACHOQQ_RS00840"
                     /old_locus_tag="pgaptmp_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGU4468483.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4190..5593
                     /locus_tag="ACHOQQ_RS00845"
                     /old_locus_tag="pgaptmp_000169"
     CDS             4190..5593
                     /locus_tag="ACHOQQ_RS00845"
                     /old_locus_tag="pgaptmp_000169"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGU4468484.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5604..6440
                     /gene="eutJ"
                     /locus_tag="ACHOQQ_RS00850"
                     /old_locus_tag="pgaptmp_000170"
     CDS             5604..6440
                     /gene="eutJ"
                     /locus_tag="ACHOQQ_RS00850"
                     /old_locus_tag="pgaptmp_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HGU4468485.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRIPLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6430..7617
                     /gene="eutG"
                     /locus_tag="ACHOQQ_RS00855"
                     /old_locus_tag="pgaptmp_000171"
     CDS             6430..7617
                     /gene="eutG"
                     /locus_tag="ACHOQQ_RS00855"
                     /old_locus_tag="pgaptmp_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGU4468486.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVCLYA
                     AAQ"
     gene            7834..9060
                     /gene="eutH"
                     /locus_tag="ACHOQQ_RS00860"
                     /old_locus_tag="pgaptmp_000172"
     CDS             7834..9060
                     /gene="eutH"
                     /locus_tag="ACHOQQ_RS00860"
                     /old_locus_tag="pgaptmp_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGU4468487.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHILGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            9057..10460
                     /gene="eutA"
                     /locus_tag="ACHOQQ_RS00865"
                     /old_locus_tag="pgaptmp_000173"
     CDS             9057..10460
                     /gene="eutA"
                     /locus_tag="ACHOQQ_RS00865"
                     /old_locus_tag="pgaptmp_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGU4468488.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10472..11833
                     /gene="eutB"
                     /locus_tag="ACHOQQ_RS00870"
                     /old_locus_tag="pgaptmp_000174"
     CDS             10472..11833
                     /gene="eutB"
                     /locus_tag="ACHOQQ_RS00870"
                     /old_locus_tag="pgaptmp_000174"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGU4468489.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11854..12741
                     /gene="eutC"
                     /locus_tag="ACHOQQ_RS00875"
                     /old_locus_tag="pgaptmp_000175"
     CDS             11854..12741
                     /gene="eutC"
                     /locus_tag="ACHOQQ_RS00875"
                     /old_locus_tag="pgaptmp_000175"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HGU4468490.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCTTTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12751..13410
                     /gene="eutL"
                     /locus_tag="ACHOQQ_RS00880"
                     /old_locus_tag="pgaptmp_000176"
     CDS             12751..13410
                     /gene="eutL"
                     /locus_tag="ACHOQQ_RS00880"
                     /old_locus_tag="pgaptmp_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGU4468491.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13423..13923
                     /gene="eutK"
                     /locus_tag="ACHOQQ_RS00885"
                     /old_locus_tag="pgaptmp_000177"
     CDS             13423..13923
                     /gene="eutK"
                     /locus_tag="ACHOQQ_RS00885"
                     /old_locus_tag="pgaptmp_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGU4468492.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13969..15021
                     /gene="eutR"
                     /locus_tag="ACHOQQ_RS00890"
                     /old_locus_tag="pgaptmp_000178"
     CDS             13969..15021
                     /gene="eutR"
                     /locus_tag="ACHOQQ_RS00890"
                     /old_locus_tag="pgaptmp_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGU4468493.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(15027..15926)
                     /gene="hemF"
                     /locus_tag="ACHOQQ_RS00895"
                     /old_locus_tag="pgaptmp_000179"
     CDS             complement(15027..15926)
                     /gene="hemF"
                     /locus_tag="ACHOQQ_RS00895"
                     /old_locus_tag="pgaptmp_000179"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGU4468494.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGTEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRKEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15930..16799)
                     /gene="amiA"
                     /locus_tag="ACHOQQ_RS00900"
                     /old_locus_tag="pgaptmp_000180"
     CDS             complement(15930..16799)
                     /gene="amiA"
                     /locus_tag="ACHOQQ_RS00900"
                     /old_locus_tag="pgaptmp_000180"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGU4468495.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            17013..17438
                     /locus_tag="ACHOQQ_RS00905"
                     /old_locus_tag="pgaptmp_000181"
     CDS             17013..17438
                     /locus_tag="ACHOQQ_RS00905"
                     /old_locus_tag="pgaptmp_000181"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGU4468496.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17425..17874
                     /locus_tag="ACHOQQ_RS00910"
                     /old_locus_tag="pgaptmp_000182"
     CDS             17425..17874
                     /locus_tag="ACHOQQ_RS00910"
                     /old_locus_tag="pgaptmp_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGU4468497.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGFGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            17935..18510
                     /locus_tag="ACHOQQ_RS00915"
                     /old_locus_tag="pgaptmp_000183"
     CDS             17935..18510
                     /locus_tag="ACHOQQ_RS00915"
                     /old_locus_tag="pgaptmp_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGU4468498.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18606..19505
                     /gene="yfeX"
                     /locus_tag="ACHOQQ_RS00920"
                     /old_locus_tag="pgaptmp_000184"
     CDS             18606..19505
                     /gene="yfeX"
                     /locus_tag="ACHOQQ_RS00920"
                     /old_locus_tag="pgaptmp_000184"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGU4468499.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19563..20867)
                     /gene="pbp4b"
                     /locus_tag="ACHOQQ_RS00925"
                     /old_locus_tag="pgaptmp_000185"
     CDS             complement(19563..20867)
                     /gene="pbp4b"
                     /locus_tag="ACHOQQ_RS00925"
                     /old_locus_tag="pgaptmp_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HGU4468500.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20872..22296)
                     /gene="murP"
                     /locus_tag="ACHOQQ_RS00930"
                     /old_locus_tag="pgaptmp_000186"
     CDS             complement(20872..22296)
                     /gene="murP"
                     /locus_tag="ACHOQQ_RS00930"
                     /old_locus_tag="pgaptmp_000186"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGU4468501.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSSQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22300..23196)
                     /gene="murQ"
                     /locus_tag="ACHOQQ_RS00935"
                     /old_locus_tag="pgaptmp_000187"
     CDS             complement(22300..23196)
                     /gene="murQ"
                     /locus_tag="ACHOQQ_RS00935"
                     /old_locus_tag="pgaptmp_000187"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGU4468502.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRKVLDKE"
     gene            23360..24217
                     /gene="murR"
                     /locus_tag="ACHOQQ_RS00940"
                     /old_locus_tag="pgaptmp_000188"
     CDS             23360..24217
                     /gene="murR"
                     /locus_tag="ACHOQQ_RS00940"
                     /old_locus_tag="pgaptmp_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGU4468503.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKQLALEQTCALFDFARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24346..25137
                     /gene="ucpA"
                     /locus_tag="ACHOQQ_RS00945"
                     /old_locus_tag="pgaptmp_000189"
     CDS             24346..25137
                     /gene="ucpA"
                     /locus_tag="ACHOQQ_RS00945"
                     /old_locus_tag="pgaptmp_000189"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGU4468504.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPVSVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25441..26457
                     /gene="cysP"
                     /locus_tag="ACHOQQ_RS00950"
                     /old_locus_tag="pgaptmp_000190"
     CDS             25441..26457
                     /gene="cysP"
                     /locus_tag="ACHOQQ_RS00950"
                     /old_locus_tag="pgaptmp_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGU4468505.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26457..27290
                     /gene="cysT"
                     /locus_tag="ACHOQQ_RS00955"
                     /old_locus_tag="pgaptmp_000191"
     CDS             26457..27290
                     /gene="cysT"
                     /locus_tag="ACHOQQ_RS00955"
                     /old_locus_tag="pgaptmp_000191"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGU4468506.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27290..28165
                     /gene="cysW"
                     /locus_tag="ACHOQQ_RS00960"
                     /old_locus_tag="pgaptmp_000192"
     CDS             27290..28165
                     /gene="cysW"
                     /locus_tag="ACHOQQ_RS00960"
                     /old_locus_tag="pgaptmp_000192"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGU4468507.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            28155..29252
                     /gene="cysA"
                     /locus_tag="ACHOQQ_RS00965"
                     /old_locus_tag="pgaptmp_000193"
     CDS             28155..29252
                     /gene="cysA"
                     /locus_tag="ACHOQQ_RS00965"
                     /old_locus_tag="pgaptmp_000193"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGU4468508.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDNAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29372..30283
                     /gene="cysM"
                     /locus_tag="ACHOQQ_RS00970"
                     /old_locus_tag="pgaptmp_000194"
     CDS             29372..30283
                     /gene="cysM"
                     /locus_tag="ACHOQQ_RS00970"
                     /old_locus_tag="pgaptmp_000194"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGU4468509.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQRAGI"
     gene            complement(30286..30654)
                     /locus_tag="ACHOQQ_RS00975"
                     /old_locus_tag="pgaptmp_000195"
     CDS             complement(30286..30654)
                     /locus_tag="ACHOQQ_RS00975"
                     /old_locus_tag="pgaptmp_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HGU4468510.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30759..31610
                     /gene="pdxK"
                     /locus_tag="ACHOQQ_RS00980"
                     /old_locus_tag="pgaptmp_000196"
                     /gene_synonym="thiJ"
     CDS             30759..31610
                     /gene="pdxK"
                     /locus_tag="ACHOQQ_RS00980"
                     /old_locus_tag="pgaptmp_000196"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGU4468511.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLVEA"
     gene            complement(31653..32162)
                     /gene="crr"
                     /locus_tag="ACHOQQ_RS00985"
                     /old_locus_tag="pgaptmp_000197"
     CDS             complement(31653..32162)
                     /gene="crr"
                     /locus_tag="ACHOQQ_RS00985"
                     /old_locus_tag="pgaptmp_000197"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGU4468512.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32203..33930)
                     /gene="ptsI"
                     /locus_tag="ACHOQQ_RS00990"
                     /old_locus_tag="pgaptmp_000198"
     CDS             complement(32203..33930)
                     /gene="ptsI"
                     /locus_tag="ACHOQQ_RS00990"
                     /old_locus_tag="pgaptmp_000198"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGU4468513.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33975..34232)
                     /gene="ptsH"
                     /locus_tag="ACHOQQ_RS00995"
                     /old_locus_tag="pgaptmp_000199"
     CDS             complement(33975..34232)
                     /gene="ptsH"
                     /locus_tag="ACHOQQ_RS00995"
                     /old_locus_tag="pgaptmp_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGU4468514.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34616..35587)
                     /gene="cysK"
                     /locus_tag="ACHOQQ_RS01000"
                     /old_locus_tag="pgaptmp_000200"
     CDS             complement(34616..35587)
                     /gene="cysK"
                     /locus_tag="ACHOQQ_RS01000"
                     /old_locus_tag="pgaptmp_000200"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGU4468515.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDIFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35772..36533)
                     /gene="cysZ"
                     /locus_tag="ACHOQQ_RS01005"
                     /old_locus_tag="pgaptmp_000201"
     CDS             complement(35772..36533)
                     /gene="cysZ"
                     /locus_tag="ACHOQQ_RS01005"
                     /old_locus_tag="pgaptmp_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGU4468516.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36763..37761
                     /gene="zipA"
                     /locus_tag="ACHOQQ_RS01010"
                     /old_locus_tag="pgaptmp_000202"
     CDS             36763..37761
                     /gene="zipA"
                     /locus_tag="ACHOQQ_RS01010"
                     /old_locus_tag="pgaptmp_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGU4468517.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37832..39847
                     /gene="ligA"
                     /locus_tag="ACHOQQ_RS01015"
                     /old_locus_tag="pgaptmp_000203"
     CDS             37832..39847
                     /gene="ligA"
                     /locus_tag="ACHOQQ_RS01015"
                     /old_locus_tag="pgaptmp_000203"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGU4468518.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39849..40067
                     /locus_tag="ACHOQQ_RS01020"
                     /old_locus_tag="pgaptmp_000204"
     CDS             39849..40067
                     /locus_tag="ACHOQQ_RS01020"
                     /old_locus_tag="pgaptmp_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGU4468519.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(40064..41062)
                     /locus_tag="ACHOQQ_RS01025"
                     /old_locus_tag="pgaptmp_000205"
     CDS             complement(40064..41062)
                     /locus_tag="ACHOQQ_RS01025"
                     /old_locus_tag="pgaptmp_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGU4468520.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            41152..42078
                     /locus_tag="ACHOQQ_RS01030"
                     /old_locus_tag="pgaptmp_000206"
     CDS             41152..42078
                     /locus_tag="ACHOQQ_RS01030"
                     /old_locus_tag="pgaptmp_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4468521.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(42069..42410)
                     /locus_tag="ACHOQQ_RS01035"
                     /old_locus_tag="pgaptmp_000207"
     CDS             complement(42069..42410)
                     /locus_tag="ACHOQQ_RS01035"
                     /old_locus_tag="pgaptmp_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HGU4468522.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(42530..42655)
                     /locus_tag="ACHOQQ_RS01040"
                     /old_locus_tag="pgaptmp_000208"
     ncRNA           complement(42530..42655)
                     /ncRNA_class="other"
                     /locus_tag="ACHOQQ_RS01040"
                     /old_locus_tag="pgaptmp_000208"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(42686..42761)
                     /locus_tag="ACHOQQ_RS01045"
                     /old_locus_tag="pgaptmp_000209"
     tRNA            complement(42686..42761)
                     /locus_tag="ACHOQQ_RS01045"
                     /old_locus_tag="pgaptmp_000209"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42726..42728),aa:Lys,seq:ttt)
     gene            complement(42766..42841)
                     /locus_tag="ACHOQQ_RS01050"
                     /old_locus_tag="pgaptmp_000210"
     tRNA            complement(42766..42841)
                     /locus_tag="ACHOQQ_RS01050"
                     /old_locus_tag="pgaptmp_000210"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42806..42808),aa:Val,seq:tac)
     gene            complement(42888..42963)
                     /locus_tag="ACHOQQ_RS01055"
                     /old_locus_tag="pgaptmp_000211"
     tRNA            complement(42888..42963)
                     /locus_tag="ACHOQQ_RS01055"
                     /old_locus_tag="pgaptmp_000211"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42928..42930),aa:Val,seq:tac)
     gene            complement(43008..43083)
                     /locus_tag="ACHOQQ_RS01060"
                     /old_locus_tag="pgaptmp_000212"
     tRNA            complement(43008..43083)
                     /locus_tag="ACHOQQ_RS01060"
                     /old_locus_tag="pgaptmp_000212"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43048..43050),aa:Val,seq:tac)
     gene            43342..44757
                     /gene="gltX"
                     /locus_tag="ACHOQQ_RS01065"
                     /old_locus_tag="pgaptmp_000213"
     CDS             43342..44757
                     /gene="gltX"
                     /locus_tag="ACHOQQ_RS01065"
                     /old_locus_tag="pgaptmp_000213"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGU4468523.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(44809..45201)
                     /locus_tag="ACHOQQ_RS01070"
                     /old_locus_tag="pgaptmp_000214"
     CDS             complement(44809..45201)
                     /locus_tag="ACHOQQ_RS01070"
                     /old_locus_tag="pgaptmp_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4468524.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(45203..45562)
                     /locus_tag="ACHOQQ_RS01075"
                     /old_locus_tag="pgaptmp_000215"
     CDS             complement(45203..45562)
                     /locus_tag="ACHOQQ_RS01075"
                     /old_locus_tag="pgaptmp_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4468525.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            45783..45858
                     /locus_tag="ACHOQQ_RS01080"
                     /old_locus_tag="pgaptmp_000216"
     tRNA            45783..45858
                     /locus_tag="ACHOQQ_RS01080"
                     /old_locus_tag="pgaptmp_000216"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45816..45818,aa:Ala,seq:ggc)
     gene            45898..45973
                     /locus_tag="ACHOQQ_RS01085"
                     /old_locus_tag="pgaptmp_000217"
     tRNA            45898..45973
                     /locus_tag="ACHOQQ_RS01085"
                     /old_locus_tag="pgaptmp_000217"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45931..45933,aa:Ala,seq:ggc)
     gene            46181..48370
                     /locus_tag="ACHOQQ_RS01090"
                     /old_locus_tag="pgaptmp_000218"
     CDS             46181..48370
                     /locus_tag="ACHOQQ_RS01090"
                     /old_locus_tag="pgaptmp_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU4468526.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(48420..49622)
                     /gene="nupC"
                     /locus_tag="ACHOQQ_RS01095"
                     /old_locus_tag="pgaptmp_000219"
     CDS             complement(48420..49622)
                     /gene="nupC"
                     /locus_tag="ACHOQQ_RS01095"
                     /old_locus_tag="pgaptmp_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGU4468527.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT        11659 a        12664 c        13795 g        11714 t
ORIGIN      
        1 agtttattca taacagtaaa tatctctcca ttcttagtga tctacctcac cttttaaacg
       61 cgcttgccga attttgttat ttactctgac gaaaaattgt cacgatacac gcaagttttt
      121 cacaggcggc gactcatgga taaagaacgc atcattcagg aatttgtgcc cggcaaacag
      181 gtcacgctgg cgcatctcat cgcgcatccc ggcgaggaac tggcgaagaa aatcggcgtt
      241 ccggatgcgg gcgcaattgg cattatgacg ctaactcctg gcgaaacggc gatgatcgcc
      301 ggtgatttag cgctgaaagc tgccgatgtg catatcggtt ttctcgaccg ttttagcggc
      361 gcgctggtga tttatggctc ggtgggggca gtagaagaag cgttatcaca aaccgtcagt
      421 ggtttaggtc gcttattaaa ttatacgctt tgtgaaatga ccaaaagtta atacgaggca
      481 ttcatgaaac gaattgcgtt tgtgggtacc gtcggggcag ggaaaacaac gttatttaat
      541 gtgttgcagg ggaattatac cctcgccaga aaaacacagg ccgtggaatt taatgataag
      601 ggcgatattg atacgccggg tgaatatttt agccatcccc gctggtatca cgccttaatt
      661 accacgctac aggatgtgga catgctgatc tatgtccacg gcgcgaatga cccggaaagt
      721 cggttgcctg ccgggttgct ggatattggc gtcagcaaac ggcaaatcgc cgtcatcagt
      781 aaaacggaca tgccggatgc tgacgtcgcc gcaacgcgaa agttattgct tgagacgggc
      841 ttcgaggagc cgattttcga gctaaacagc cacgacccgc aaagcgtgca gcaactggtg
      901 gattatctgg catctctcac caaacaggag gaagcaggtg aaaaaactca tcacagcgaa
      961 tgatattcgt gaagcacacg cgcgcggcga acaggcaatg tccgtggttc tgcgcgccag
     1021 cattattacc ccggaagccc gtgaagtggc ggatctactg ggttttacca tcaccgaatg
     1081 tgatgaatcg tccccggtaa cggcgtccgt tcccgccagc gtgccagccg ataaaaccga
     1141 aagtcagcgc atccgcgaaa ccatcattgc ccagctgccg gaaggccagt ttaccgaaag
     1201 cctggtcgcg cagttgatgg aaaaagtgat gaaggaaaaa cagtcgctgg agcagggtgc
     1261 aatgcagccg agcttcaaat cggtgaccgg caaaggcggc atcaaagtga ttgatggtag
     1321 cagcgtcaag tttggtcgtt tcgacggcgc agagccgcat tgcgtgggct taactgacct
     1381 tgtgaccgga gacgacggca gcagcatggc cgccggtttt atgcagtggg acaacgcatt
     1441 cttcccgtgg accctgaact acgacgaaat cgatatggtg ctggaagggg agctgcatgt
     1501 tcgccacgca ggagaaacca tgatcgccaa agcgggcgat gtgatgttta tcccgaaagg
     1561 ctccagcatc gaatttggta cgacatccag cgtgaaattc ctgtatgtcg cctggccggc
     1621 taactggcaa tccctatgaa agatttcatc accgaagcat ggctaagagc gaaccatacg
     1681 ctcagcgaag gcgcagagat ccatctccct gcggacagtc gcctgacgcc ctctgcccgg
     1741 gagttactgg aaagccgcca tctgcgcatc aagtttattg acgagcaggg ccgcctgttt
     1801 gttgacgatg agcagcagca gccacagccc gttcacgggc tgaccagtag cgatgaacat
     1861 ccgcaggcgt gctgcgaact gtgccgccag ccggtggcaa aaaagccgga tacgctgact
     1921 catctgtcgg cggaaaaaat ggtcgctaaa agcgatccgc gtctgggttt tcgcgcggtt
     1981 ctcgacagca ccattgcgct ggcagtgtgg ctgcaaattg aactggcgga accgtggcag
     2041 ccgtggctgg cggatatccg ttcgcgtctt ggcaacatta tgcgcgccga tgcgctgggg
     2101 gaaccgctgg gctgccaggc gattgtcggg ctttctgacg aagatctgca ccggctttct
     2161 catcagccgt tgcgttatct tggtcacgat catctggtgc cggaagccag ccacgggcgc
     2221 gatgcggcgt tgctcaatct gctacgcacc aaagtgcggg aaaccgaaac cgtagcggcg
     2281 caggtgttta tcacccgcag ctttgaagta ttacgcccgg acatcttgca ggcgctgaac
     2341 cgcctttcga gcacggtcta cgtgatgatg atcctgagcg taacgaagca gccgctaacg
     2401 gtgaagcaga tccaacaacg actgggggaa acgcaatgat tattgaacgt tgtcgtgaac
     2461 tggcgttgcg agcgcccgcc agagtggttt ttccggatgc gttagatcaa cgtgtgctga
     2521 aagcggcgca atatttacat caacaaggtc tggcaacgcc cattctggtc gccaatccgt
     2581 ttgaacttcg tcagtttgcg ctcagtcatg gcgtagcgat ggacgggcta caggtgattg
     2641 atccgcatgg caacctcgca atgcgggaag aatttgctca tcgctggctg gcccgcgcgg
     2701 gcgaaaaaac gccgccggat gcgctggaaa aactcaccga accgctgatg ttcgccgccg
     2761 caatggtcag cgccggtata gcggatgtct gtatcgcggg caacctctct tccacggcga
     2821 atgtgctgcg tgccggatta cgcattatcg gcttgcagcc aggctgtaaa acgctctcat
     2881 ccattttcct gatgctgcca cagtacagtg gccccgcgtt gggctttgcc gattgcagcg
     2941 tggtaccaca gccgacggcg gcgcagctgg cggatatcgc gcttgccagc gccgaaacct
     3001 ggcgcgccat caccggagaa gagccgcgcg tggcgatgct gtcgttttcc agtaacggta
     3061 gcgcccgtca cccctgcgtt gccaacgtcc agcaggcgac agaaatcgtc cgtgagcgcg
     3121 caccaaagct ggttgtcgat ggcgagttgc agtttgacgc cgccttcgtg ccggaagtgg
     3181 cggcgcaaaa agcgcctgcc agcccgctac agggcaaggc caatgtgatg gtttttccgt
     3241 cgctggaagc cggaaatatt ggttacaaaa tcgcacagcg acttggcgga tatcgtgccg
     3301 tcgggccatt gatacaagga cttgccgcgc cgatgcacga tctctctcgt ggttgtagcg
     3361 tgcaggaaat tatcgagctg gcgctggtgg cagctgtgcc gcgtcagaca gaagtgaacc
     3421 gcgaaagcag tttacaaaca ctggttgaat gaacggtccc gttctggacc ctgataaaga
     3481 ggaaagcaca atggaagcat taggaatgat cgaaacccgg ggcctggttg cactgattga
     3541 ggcttctgac gccatggtga aagctgcgcg cgtcaagctg gttggtgtta agcagattgg
     3601 cggcggcctg tgtactgcca tggttcgtgg tgatgttgca gcctgtaaag cggcgaccga
     3661 tgcaggtgcc gcagcggcac agcgtatcgg cgagctggta tctgtacacg ttatcccgcg
     3721 tccgcacggc gacctggaag aagtgttccc gatcggcctg aaaggcgata gcagcaacct
     3781 gtaatcaccc tgtgtgacgt cgattcgggc gtcacgctct ctcatttttc attttacctt
     3841 ccgcggaggg tagggggagc ttttacctga aatacgccac ggaggcgggt atgaaactgg
     3901 cagtcgtcac tggacaaatt gtttgtaccg tacgccatca cggactggcg catgacaaat
     3961 tgctgatggt ggaaatgatt gatccacatg gtaatcctga cgggcaatgt gccgtcgcca
     4021 tcgacaatat tggcgcggga accggggagt gggtgttgct ggtgagtggc agttccgccc
     4081 gccaggcgca taaaagcgaa acgtcaccgg tcgatctgtg cgtgattggc atcgtcgatg
     4141 aggtggtgtc tggcggtcag gtaattttcc acaaataagg cagaacatca tgaatcaaca
     4201 ggatattgaa caggtggtga aagcggtact gctgaaaatg caaagcagtg acacgccgcc
     4261 cgccgccgtt catgagatgg gcgttttcgc gtccctggat gacgctgttg cggcagccaa
     4321 agtcgcccag caagggttaa agagcgtggc aatgcgccag ttagccattg ctgccattcg
     4381 tgaagcaggc gaaaaacacg ccagagattt agcggaactt gccgtcagtg aaaccggcat
     4441 gggtcgcgtt gaagataaat ttgccaaaaa cgtcgctcag gcacgcggca cgccaggcgt
     4501 tgagtgtctc tctccgcaag tgctgacggg cgacaacggc ctgactttaa ttgaaaacgc
     4561 gccctggggc gtggtggctt cggtgacgcc gtccactaac ccggcggcaa ccgtaattaa
     4621 taacgccatc agcctgattg ccgcgggcaa cagcgtcatt tttgccccgc atccggcggc
     4681 gaaaaaagtc tcccagcggg cgatcacgct gctcaaccag gcgattgttg ccgcaggcgg
     4741 gccggaaaac ttactggtta ctgtggcaaa tccggatatc gaaaccgcgc aacgcttgtt
     4801 caagtttccg ggtatcggcc tgctggtggt gaccggcggc gaagcggtag tagaagcggc
     4861 gcgtaaacac actaataaac gtctgattgc cgcaggcgct ggcaacccgc cggtagtggt
     4921 ggatgaaacc gctgacctcg cccgtgctgc tcagtccatc gtcaaaggcg cgtctttcga
     4981 taacaacatc atttgtgccg acgaaaaggt actgattgtt gttgatagcg tagccgatga
     5041 actgatgcgt ctgatggaag gccagcacgc ggtgaaactg accgcagaac aggcgcagca
     5101 gctgcaaccg gtgttgctga aaaatatcga cgagcgcgga aaaggcaccg tcagccgtga
     5161 ctgggttggt cgcgatgcgg cgaaaatcgc ggcggcaatc ggccttaaag ttccgcaaga
     5221 aacgcgcctg ctgtttgtgg aaaccaccgc agaacatccg tttgccgtga ctgaactgat
     5281 gatgccggtg ttgcccgtcg tgcgcgtcgc caacgtggcg gatgccattg cgctagcggt
     5341 gaaactggag ggcggttgcc accacacggc ggcaatgcac tcgcgcaata tcgagaacat
     5401 gaaccagatg gcgaatgcca ttgataccag cattttcgtt aagaacggac cgtgcattgc
     5461 cgggctgggt ctgggcgggg aaggctggac taccatgacc atcaccacgc caaccggtga
     5521 aggggtaacc agcgcgcgga cgtttgtccg tctgcgtcgc tgtgtattag tcgatgcgtt
     5581 tcgcattgtt taaggagtaa cagatggcgc acgacgaaca atggctcacc ccacgtctgc
     5641 aaacggcggc aacgctgtgt aaccagacgc ccgccgcgac ggaatctccg ctgtggctgg
     5701 gggtggatct gggcacctgc gatgtggtgt cgatggttgt cgaccgcgac ggtcagccag
     5761 tggcggtatg cctcgactgg gccgatgtgg ttcgcgacgg catcgtctgg gatttcttcg
     5821 gcgcggtcac cattgttcgt cgccatctcg acacgctcga gcagcaattc ggtcgtcgtt
     5881 ttagccatgc ggcgacttca tttcctccag gtacggaccc gcgtatttcc atcaacgtgc
     5941 tggaatctgc cggactggaa gtgagccatg tgctggatga gccaaccgca gtcgcggatc
     6001 tgctgcaact ggacaacgcc ggtgtagtgg atatcggcgg cggcactacc ggcattgcca
     6061 tcgtgaaaaa gggcaaggtg acgtactcgg cggatgaagc gactggcgga catcacatct
     6121 ctctgactct cgccggaaat cgtcgtattc cgctggaaga ggcagagcag tacaagcgcg
     6181 gtcacggtga cgagatttgg cccgcggtga aaccggtgta cgaaaaaatg gcggacattg
     6241 tcgcccggca tattgaaggg cagggcatta ctgatttatg gctggcgggc ggctcttgta
     6301 tgcaaccggg cgtggcagaa ctgtttcgca aacaattccc ggcgttacag gtgcatttac
     6361 cgcagcacag tttgtttatg accccgctgg cgatcgccag tagtgggcga gagaaagcgg
     6421 aggggctata tgcaaaatga attgcagacc gcgctctttc aggcgttcga taccctgaat
     6481 ctgcaacggg taaaaacatt tagtgttcca ccggtgacgc tttgtggtcc gggctcggtg
     6541 agcagttgcg gacagcaagc gcaaacgcgt gggctgaaac atctgttcgt gatggcagac
     6601 agctttttgc atcaggcagg gatgaccgcc gggctgacgc gtagcctggc cgttaaaggt
     6661 atcgccatga cgctctggcc atgtccggtg ggcgaaccgt gcattaccga cgtgtgtgca
     6721 gccgtggcgc agttgcgtga atcaggttgt gacggggtga tcgcatttgg cggcggctcg
     6781 gtgctggatg cggcgaaagc cgtggcgttg ctggtgacga acccggatag cacgctggca
     6841 gagatgtcag aaaccagcgt tctgcaaccg cgcttgccgc tgattgccat tccaactacc
     6901 gccggaaccg gctctgaaac cacaaatgta acggtgatta tcgacgcggt gagcgggcgc
     6961 aagcaggtgt tagcccatgc ctcgctgatg ccggatgtgg cgatcctcga cgccgcattg
     7021 accgaaggtg tgccgtcgca tgtcacggcg atgaccggca ttgatgcgtt aacccatgcc
     7081 attgaagcat acagcgccct gaacgctaca ccgtttaccg acagtctggc gattggtgcc
     7141 attgcgatga ttggcaaatc gctgccgaaa gcggtgggct acggtcacga ccttgccgcg
     7201 cgcgagagca tgttgctggc ttcatgtatg gcgggaatgg cgttttccag tgcgggtctt
     7261 gggttgtgcc acgcgatggc gcatcagccg ggcgcggcgc tgcatattcc gcacggtctg
     7321 gcgaacgcta tgttgctgcc aacggtgatg gagtttaacc ggatggtttg tcgcgaacgc
     7381 tttagtcaga ttggtcgggc actgcgaact aaaaaatccg acgatcgtga cgctattaac
     7441 gcggtaagtg agctgattgc ggaagttggg attggtaaac gactgggcga tgttggcgcg
     7501 acatctgcgc attacggtgc atgggcgcag gccgcgcagg aagatatttg tctgcgcagc
     7561 aacccgcgta ccgccagcct ggagcagatt gtctgcctgt acgcagcggc gcaataaatg
     7621 ccggatgcga cgcttgccgc gtcttatccg gcctacggga ttgcacatgt agagcggata
     7681 aagcgttcac gccgcatccg ccagtggcgc ggtgcaattg ccggatgcga cgcttgccgc
     7741 gtcttatccg gcctacggga ttgcacatgt agggcggata aggcgttcac gcagcatccg
     7801 ctaataaata acaactaaca gggagtaaag gcgatgggaa ttaacgaaat catcatgtac
     7861 atcatgatgt tctttatgct gatagctgcc gtagacagga tcctgtcaca gttcggcggt
     7921 tctgctcgtt tcctcggtaa gttcggtaaa agtatcgaag gatcaggcgg tcagttcgaa
     7981 gaaggcttta tggcaatggg cgcactgggc ctggcgatgg tcggtatgac cgcgctggca
     8041 ccggtactgg ctcacatact cgggccggta attatcccgg tttacgaaat gctcggcgca
     8101 aacccgtcaa tgttcgccgg aacgctgctg gcgtgcgata tgggcggctt cttcctcgcc
     8161 aaagagctgg cgggcggcga cgtagcagcg tggctatact cagggttaat tctcgggtcg
     8221 atgatggggc caacgattgt gttttccatt ccggtggcgc tcggcattat cgaaccttct
     8281 gaccgtcgtt atctggcgct cggcgtgctg gcgggcattg tgaccattcc gattggctgt
     8341 attgctggtg gtctggtagc tatgtactcc ggtgtgcaga tcaacggcca gccggtggaa
     8401 ttcactttcg ccctgatcct gatgaacatg atcccggtga tcattgttgc gattctggtg
     8461 gcgctggggc tgaaattcat cccggaaaaa atgatcaacg gcttccagat cttcgccaaa
     8521 ttcctcgttg cattgatcac cctcggtctt gccgctgcgg tagtgaaatt cctgcttggc
     8581 tgggaactga tccccggtct ggatcctatc tttatggccc ctggcgataa acccggtgag
     8641 gtgatgcgcg ccattgaagt tatcggttct atctcctgcg ttctgttagg ggcgtatccg
     8701 atggtgctgc tgctgacccg ctggtttgaa aaaccgctga tgagcgtcgg taaagtgctg
     8761 aatatgaaca acatcgcggc agccggcatg gtggcaacgc ttgccaacaa catcccgatg
     8821 ttcggcatga tgaagcagat ggatacccgc ggcaaagtca tcaactgcgc cttcgccgtt
     8881 tccgctgctt tcgccctggg cgaccactta ggcttcgccg ctgccaacat gaatgcaatg
     8941 atcttcccga tgattgtcgg caagttgatc ggcggcgtaa cggcgattgg cgtggcgatg
     9001 atgctggtgc caaaagacga cgcgaccgcg gctaaaaccg aagcggaggc acaatcgtga
     9061 acactcgcca gctactgagc gtcggtatcg atatcggcac caccaccacc caggtgattt
     9121 tctcccacct ggaactggtt aaccgtgcgg cggtgtcgca ggtgccgcgc tacgaattca
     9181 tcaaacgcga aattagctgg caaagcccgg tgttctttac ccctgtcgat aaacagggtg
     9241 gtttaaaaga agcggaactg aaatcgctga ttttagagca atatcaggct gcgggcattg
     9301 cgccggaaag cgttgattct ggtgccatca tcatcaccgg tgaaagcgcg aaaacccgca
     9361 atgctcgccc ggcggtgatg gcgctctctc agtcgctggg cgattttgtc gttgccagcg
     9421 ccgggccgca cctcgaatcc gtgatcgccg gtcacggagc cggggcacaa accctttctg
     9481 aacaacggct gtgtcgggta ctgaatatcg acatcggcgg tggcaccgcg aactacgccc
     9541 tgttcgatgc cggaaaaatc agcggcactg cctgcctcaa cgtcggtggt cgcctgctgg
     9601 aaaccgacag ccaggggcgc gtggtttacg ctcataaacc ggggcagatg attgtggatg
     9661 agtgcttcgg cgcaggcacc gacgcccgtt cgctgaccgg cgctcagctg gtgcaggtta
     9721 cccggcggat ggcagagctg attgtcgaag tgattgacgg aacgctttcg ccgctcgcgc
     9781 aggcattaat gcaaaccggt ttgctgcccg caggtgttac gcccgaaatc attacgcttt
     9841 ctggcggcgt gggcgaatgt tatcgccacc agcccgccga cccgttctgt tttgccgata
     9901 ttggcccgct gctggcaacg gcgctgcatg accatccgcg cctgcgtgag atgaacgtac
     9961 agtttccggc gcaaaccgtg cgcgccacgg tgattggtgc gggtgcacat accctttcgc
    10021 tctctggcag cacaatctgg cttgagggcg tacaactgcc gctgcgcaat ttgccggtgg
    10081 cgatcccaat tgatgaaacg gatctggtga gtgcctggca acaggcgctg cttcagctgg
    10141 atcttgatcc caaaactgac gcgtacgtgc tggcgcttcc cgcctcgctg cctgtgcgtt
    10201 acgccgcggt actgacggtc atcaacgcgt tggtcgattt cgtcgcgcgt tttccgaatc
    10261 cgcatcccct gctggtggtg gccgggcagg actttggtaa agctctgggc atgttgttgc
    10321 gcccacagct acaacaactc ccgttggcag tcattgacga agtgattgtc cgcgcggggg
    10381 actatatcga cattggtacg cctctttttg gcggatcggt tgtgccggtg acggtgaaat
    10441 cactcgcatt tccttcctga gggaacgact tatgaaacta aagaccacat tgttcggcaa
    10501 tgtatatcag tttaaggatg taaaagaggt gctggctaaa gccaacgaac tgcgttcggg
    10561 ggatgtgctg gcgggcgtcg cagcggcaag ctcacaggag cgcgtggcgg caaagcaggt
    10621 gttgtcggaa atgaccgtag cggacatccg caataatccg gtgattgcct atgaagatga
    10681 ctgcgtgacg cggctgattc aggacgatgt taacgaaacg gcctacaacc agattaaaaa
    10741 ctggagcatc agcgaactgc gtgagtatgt gctgagcgat gaaaccagcg tggacgacat
    10801 tgcctttacc cgcaaagggc tgacctcgga agtggtcgcg gcggtagcga agatttgctc
    10861 caacgcggac ctgatctacg gcgcgaagaa aatgccggta atcaaaaagg ccaataccac
    10921 catcggtatt ccgggcacct ttagcgcccg tttgcagcca aatgacaccc gtgacgacgt
    10981 gcaaagtatc gccgcgcaaa tctacgaagg gctttccttc ggggtgggcg atgcggtgat
    11041 cggcgttaac ccggtgactg acgatgtgga aaacttaagc cgcgtgctgg ataccattta
    11101 tggcgtgatc gacaaattca acatcccaac tcagggctgc gtactggcgc acgtcaccac
    11161 ccagatcgaa gcgattcgtc gcggcgcgcc tggcggactg attttccaga gtatctgtgg
    11221 cagcgaaaaa gggctgaaag agtttggcgt ggaactggcg atgctcgacg aagcgcgcgc
    11281 agtgggcgca gagttcaacc gtatcgccgg ggaaaactgc ctctacttcg aaaccggaca
    11341 aggctctgcg ctatccgctg gcgctaactt cggcgctgac caggtgacga tggaagcacg
    11401 taactatggg ctggcgcgtc attacgatcc gtttatcgtc aacaccgtgg tcggttttat
    11461 tgggccggag tatctctaca acgaccgcca gattatccgt gcgggcttag aagatcactt
    11521 tatgggcaag ctgagcggca tctctatggg ctgtgactgc tgttacacca accacgctga
    11581 cgctgaccag aacctcaacg aaaacctgat gatcctgctc gccaccgcag gctgcaacta
    11641 catcatgggg atgccgttgg gcgatgacat catgctcaac taccagacca ccgcattcca
    11701 cgataccgcc actgtgcgtc agttactcaa cctgcgtccg tcaccggagt ttgaacgctg
    11761 gctggaaagc atgggcatta tggcaaacgg tcgcctgacc aaacgggcgg gcgatccgtc
    11821 actgttcttc tgatgacgcg gagataacac atcatggatc aaaaacagat tgaagaaatt
    11881 gtacgcagcg tgatggcgtc aatgggacaa acggcccccg cgccgtcaga agcaaagtgc
    11941 accaccacca cctgtgcggc accggtgacc tcggaaagct gcgcgctgga tttaggttct
    12001 gcggaagcaa aagcgtggat tggtgttgaa aacccgcatc gcgcagacgt attaacagaa
    12061 ctgcgccgca gcaccgtggc ccgcgtttgt accggtcgtg ccggtccgcg tccacgtacg
    12121 caggcgctgc tgcgtttcct ggccgatcac tctcgttcga aagataccgt actgaaagaa
    12181 gtgccggaag agtgggtgaa agcacaaggc ttgctggaag tccgctccga aattagcgac
    12241 aaaaatctct acctgactcg cccggatatg ggccgccgcc tgtgtgcaga agctgttgaa
    12301 gcgctgaaag cgcagtgtgt tgccaatccg gacgtgcagg tcgttatttc tgatggcctg
    12361 tcaactgatg cgatcaccgt gaactacgaa gagatcctgc caccgctgat ggcgggcctg
    12421 aaacaggccg gtctgaaagt cggtacacca ttctttgttc gttatggtcg cgtgaagatt
    12481 gaagatcaga tcggtgagat cctcggcgcg aaagtagtga tcctgctggt gggcgaacgt
    12541 ccggggttgg gtcagtcaga aagcctctcc tgctacgccg tttactcgcc gcgtatggcg
    12601 accaccgttg aggccgatcg cacctgcatc tctaacattc accagggcgg cacgccgccg
    12661 gttgaagccg cagccgtcat tgtggatttg gcgaaacgta tgctggagca gaaagcatcc
    12721 ggcatcaaca tgacccgata aggaggcatc atgccagctt tagatttgat tcgaccgtcg
    12781 gtaaccgcca tgcgggtgat tgcctccgtt aacgccgatt ttgcgcgtga actgaaattg
    12841 ccgccgcata ttcgtagcct cggtctgatt tctgccgact ccgatgacgt cacctacatt
    12901 gccgctgatg aagcgaccaa gcaggcgatg gtggaagtgg tgtatggccg ctcgctgtat
    12961 gcgggcgctg cacacggccc gtcaccgact gccggtgaag tgctgattat gcttggtggg
    13021 ccaaacccgg cggaagtgcg tgccggtctg gatgcgatgg ttgcgcatat tgaaaatggc
    13081 gctgctttcc agtgggctaa tgatgcgcaa gatacggcat tcctggcaca tgtagtttcg
    13141 cgtaccggtt cttatctctc atcaaccgcc gggatcacgc ttggcgatcc gatggcgtat
    13201 ctggtggcac cgccgctgga agcgacctac ggcattgatg cagcgttgaa atctgccgac
    13261 gtacagctgg tgacctatgt cccgccaccg tctgaaacta actactcggc agcattttta
    13321 accggtagcc aggcagcgtg taaagcggcc tgtaacgcct ttaccgatgc agtgctggaa
    13381 atcgcgcgta atccaatcca gcgtgcgtaa tggaggttgc cgatgatcaa tgcactggga
    13441 ttgctggaag tggacggcat ggtcgctgcg atagatgcag cggatgccat gctcaaagca
    13501 gctaacgttc gtctgctcag tcacgaagtg cttgaccctg gtcgcttaac gctggtggtg
    13561 gaaggcgatc tggcggcgtg tcgtgctgcg ctggacgcag gttgtgctgc cgcgatgcgt
    13621 accgggcgtg tcatcagccg caaggagatc ggtcggccag acgatgacac ccagtggctg
    13681 gttactggct ttaaccgcca gccgaagcaa cccgtaaagg aacccgacgc gccagttatc
    13741 gtcgcggaat ctgctgacga gttgttggcg ctgttaacat cagtacgtca gggaatgacg
    13801 gcaggagaag tggctgccca ctttggctgg ccgctggaaa aagccagaaa tgcgctcgaa
    13861 cagctctttt ctgccgggac gttacgtaaa cgcagtagtc gttatcgtct caagccccat
    13921 taacctgtcg gaggtgccgg gtgtcctaca acacccggca ttaacatcat gaaaaagacc
    13981 cgtacagcca atttgcacca tctttatcat gaacccttac ccgaaaacct gaagctcacg
    14041 ccgaaggtcg aagtggataa tgttcatcaa cgacagacaa cggatgtcta tgaacatgct
    14101 ttaacgatta ccgcctggca gcagatttac gatcagctgc atccgggcaa gtttcatggt
    14161 gaatttacgg aaattctact cgatgatatt caggtttttc gtgaatacac cggtctggcg
    14221 ctgcgtcagt cgtgcctggt ctggcctaac tcgttctggt ttggcattcc ggcgacgcgc
    14281 ggtgagcagg gatttatcgg ttcgcaatgt ctgggaagcg cagaaatagc gacgcgcccc
    14341 ggtggaaccg agtttgaatt aagtacgccg gacgattaca cgatcctggg cgtagtgctg
    14401 tctgaagatg tcatcacccg tcaggctaac tttttgcaca atccggatcg ggtattacat
    14461 atgctgcgta gccaatcggc gctggaagtg aaagagcagc ataaagccgc gctgtggggc
    14521 tttgtccaac aggcgctggc gacgttttgc gagaacccgg aaaatctcca tcagcctgca
    14581 gtgcgaaaag tgctggggga taatttgcta atggcgatgg gggcgatgct ggaagaagcg
    14641 caaccaatgg tgacggcgga aagcatcagt catcagagtt accgtagatt actttcccgc
    14701 gcccgtgaat atgtgctgga aaacatgtcc gaaccggtga cggtgctgga cttgtgtaat
    14761 caactgcatg tcagccgccg cacgctacaa aacgcgtttc acgctatttt aggcattggc
    14821 ccgaacgcgt ggctgaaacg cattcgcctg aacgccgtac gccgcgaact gataagcccg
    14881 tggtcgcaaa gcacaacggt aaaagacgcc gccatgcagt ggggattctg gcatctgggg
    14941 caatttgcca cggattacca gcagctgttt gccgagaagc cgtcgttgac gttgcatcag
    15001 cggatgcggg aatgggggtg agggagttac acccaatccc tgaccttaat aaactcactt
    15061 aacgccgctt ctgggctgcc atcttttggc tgataatcat actcccagcg taccagtggc
    15121 ggcattgaca tcaggatcga ctcggtgcgc ccgccagttt gcaggccaaa cagagtgccg
    15181 cgatcccaga ccagattgaa ctcgacataa cgaccgcgac ggtagagctg aaaattgcgc
    15241 tcgcgctcgc cgtaggccat cgctttccgt cgctctacaa ttggtaaata agcgtcggtg
    15301 tagcctttgc ctaccgcctg cataaaggca aaacagtggt cgaaatctgg cgtgttcaga
    15361 tcatcaaaga acagcccgcc aatgccgcgc tgttctttgc gatgtttaag gtagaagtat
    15421 tcgtcgcacc actttttgta acggggataa acgtcttcgc caaacggcag gcacaggtcg
    15481 cgggcggtgc ggtgccagtg aatggcatct tcttcaaaac catagaaagg ggttaaatcg
    15541 aagccgccgc caaaccacca gactggctct gcacccggtt tttcggcaat aaaaaagcgc
    15601 acattcgcgt ggctggtggg aacatacggg ttatgcggat gcactaccag tgaaacgccc
    15661 atcgcctcga aactgcgccc ggcaagttcc gggcgatgag cggtggcgga agcaggcatc
    15721 gcctcaccgt ggacgtgaga aaagttgacg cccgcctgtt cgaaaacacc accattacgc
    15781 aacacccgac tacgcccgcc gccgccacct tcgcgctgcc aactatcttc gacaaattct
    15841 gtgccatcga cggcggtcaa ctgctgacaa atcgtatcct gaaggttgag cagaaactgt
    15901 ttaacctggt gtgcgtcggg tttcataact tatcgctttt tcgaatgtgc tttctggttg
    15961 tcgaaccagt ggaaataact gatcacgcct tcagcaatcg ctgtggcgat tttctgacga
    16021 aacgccgccg tgcctaacag ccgctcttct tccgggttgg tgataaacga ggtttccacc
    16081 agcaccgaag gaaccgacgg cgatttcaac accacaaacg cggcttgttc ggtgttgcgg
    16141 ctgtgcagtt tatgcaccgg cttaatcttc ttcagaatat gcgagccaag cgtcaggctg
    16201 tttttaatag tatcggtttg caccagatca aacagcactt gctgcaatag gtgatccttg
    16261 tcagtcgcct ttttaccggc aacttcatcg gcgcggtttt cgcgttcaga caggtatttc
    16321 gccattgcgc tacttgcccc acggttagag agggcaaata ccgaagcacc ggcagctttc
    16381 gggttggtaa agccatcggc atgaattgac ataaacagat ctgcgccatg tttatgggcg
    16441 atttcaacgc gatcgtaaag tgggataaac gtatcgccag aacgcgttaa acgcgcatca
    16501 atcccatgat tacgcaaaat ggaacggacg tttttagcaa tcgccagcac cacatgtttt
    16561 tctttcgaac cgttgcgccc gatcgctccg gtatcaatcc cgccgtggcc tggatcgaga
    16621 acaacgacac gtttgccgcc agatttttta gctttcggct tgctgtgtcc gttgctggtt
    16681 tttaaaggtt cgtctttggc gatggcttgc gacattcctg acaacgtcag ggcagccaaa
    16741 ccggctttca gcacctggcg gcgcgaagtg agtgttttta gtggtttaaa agtgctcata
    16801 cggcctgagt tgtaataata aagttccaga tgttatatcg tatcgcgtaa tccgttacga
    16861 ccgtttgctg ttgagaattg ttttactttt catttcaata gttgacaact ccccattatg
    16921 ccactttttc aaccgatttt cgtcagatat tggctaattc gaccgttcac gccataatca
    16981 gccttttaac ccgcaaaaag gtaacgaata ccatggagat acgcgtattt cgccaggaag
    17041 atttcgaaga ggtcatcacc ctttgggagc gttgcgactt gctgcgtccg tggaacgatc
    17101 cggaaatgga catcgagcgt aagatgaacc atgacgtcag tttgtttctg gtcgctgagg
    17161 tgaacggtga agtggtcgga acggtgatgg gcggttatga cgggcatcgc gggtctgctt
    17221 attatcttgg cgtgcatcca gagtttcgtg ggcgtgggat tgccaatgcg ttgcttaacc
    17281 ggctggagaa aaagctgatt gctcgcggct gcccgaaaat tcagatcaac gtgccggaag
    17341 ataacgacat ggtgctcgga atgtatgaac gcctgggata tgaacacgcc gacgtgctga
    17401 gtctgggtaa gcgtttgatt gaagatgaag agtactgagt ttcatcctgt ccattatgat
    17461 gcgcacggtc gcctgcgttt acccttgctc ttctggcttg tgctattgct tcaggcgcga
    17521 acctgggtgc tgtttgtcat tgccggtgcg tcgcgcgagc agggtactgc gctgctaaat
    17581 ctgttttatc ccgatcacga taatttctgg ctggggctga ttcctggcat tccggcggtg
    17641 ctggcgtttt tgttgagcgg tcggcgggct acgtttcctc gcacctggcg tgtactctat
    17701 tttctgttgc tgttggcgca ggtggtttta ctttgctggc aaccgtggct gtggctgaac
    17761 ggtgaatccg ttagcggttt cggtctggcg ctggtagtgg cggatattgt ggcgttaatc
    17821 tggctgctga ccaatcgacg tttacgcgct tgttttaatg aagtaaaaga ataacggcac
    17881 tttttggtga atttgcactc caagcaacgt tattgaataa ccaaaggaag tgacatgaaa
    17941 tcgctgcgtt taatgttatg cgctatgcca ttgatgctga ccggctgttc cacgatgtcg
    18001 tcagttaact ggtctgccgc taacccgtgg aactggtttg ggtcgtccac caaagtgagc
    18061 gagcagggcg ttggtgaatt aacggcgtcc acaccactgc aagaacaagc cattgccgat
    18121 gcgcttgatg gcgattatcg cctgcgcagc ggaatgaaaa ccgcgaacgg caacgtggtg
    18181 cgcttttttg aagtgatgaa gggcgacaac gtggcgatgg tgattaacgg cgatcaaggc
    18241 acgatcagtc gcattgacgt gctggatagc gatattcctg ctgacaccgg tgttaaaatc
    18301 ggtacaccgt ttagcgacct ttacagcaaa gcatttggca attgccaaaa agctgatggt
    18361 gatgataatc gtgccgtcga atgtaaagcc gaaggcagtc aacatattag ctaccagttc
    18421 agcggggaat ggagtggtcc tgaagggtta atgccttcgg acgataccct gaaaaactgg
    18481 aaagtcagta aaattatctg gcggcgttaa tttgcgtctg aacaaaccgc cgccgcgaaa
    18541 aaacacgtaa aatagcgctc aacaatgcca cggattgcgt ggcattatta ttttcaggag
    18601 gaacaatgtc tcaggttcag agtggcattt tgccagaaca ttgccgcgcg gcgatttgga
    18661 tcgaagccaa cgtgaaaggg gaagttgacg ccctgcgtgc ggccagtaaa acatttgccg
    18721 acaaactggc gacttttgaa gcgaaattcc cggacgcgca tcttggtgcg gtggttgcct
    18781 ttggtaacaa cacctggcgc gctctgagcg gcggcgttgg ggccgaagag ctgaaagatt
    18841 ttccgggcta cggtaaaggc cttgcgccaa cgacccagtt cgatgtgttg atccacattc
    18901 tttctttgcg tcacgacgta aacttctctg tcgctcaggc ggcgatggaa gctttcggcg
    18961 actgcattga agtgaaagaa gagatccacg gtttccgttg ggttgaagag cgtgacctga
    19021 gcggctttgt tgacggtacg gaaaacccgg cgggtgaaga gacgcgtcgc gaagtggcgg
    19081 ttatcaaaga cggcgtggat gcgggcggca gctatgtgtt tgtccagcgt tgggagcaca
    19141 acctgaagca gctcaaccgt atgagcgttc acgatcagga gatgatgatc gggcgcacca
    19201 aagaggccaa cgaagagatc gacggcgacg aacgcccgga aacttctcac ctgacccgcg
    19261 ttgatctgaa agaagatggc aaagggctga aaattgttcg tcagagcctg ccgtacggca
    19321 ccgccagtgg tacgcacggt ctgtacttct gcgcctactg cgcgcgtctg cataacattg
    19381 agcagcaact gctgagcatg tttggcgata ccgatggtaa gcgtgatgcg atgttgcgtt
    19441 tcaccaaacc ggtaaccggc ggctattatt tcgcaccgtc gctggacaag ttgatggcgc
    19501 tgtaagcgtt tctgattcat tgattaaagg ccaggcagta acattactga caggcctttt
    19561 tattactttt gctttaacgc cgcatacacc tgatcaacta cccaaccata cgttgcaatc
    19621 ggcagctgac cgctttcgaa catattggga ttcttttgcg gatcggcaac cggcgaatgt
    19681 ggcttgttgc ttaacatcac aatcgccata tgattcaccg gatcgataac ggtcaccgtt
    19741 ccggtccagc cagtgtgacc gtaggtttgc gggcttgcca gcgtgccaaa cgtcggcgtc
    19801 atggtggcat taccattcac gcgccagccg aggccaaaag tggcatcttc cttagagctg
    19861 gtggtgaaca tcttcaccgt ttccgcactg aacagctgca catcgccata gcccccgccg
    19921 ttcagcatcg tttgcattaa caccgcaata tcgccggtat tggaaaacaa acctgcgtgc
    19981 ccggaaacgc cgcccatcga ataaaaggct ttttcatcgt gcacctgacc ccagagagtg
    20041 gaggtgcgga tattcgggaa gtggatcact ccatcgcggg tattgccgtt caattccgtg
    20101 gcggcaattt gctgcggttt aaagcctttc agcagcgggt taaataccgt atgtgtcagg
    20161 ccgagcgggc gataaatcga ctcttcaaca tagcggtcaa gcggctgacc ggtgaccgac
    20221 tcgacgataa atccaagcag catataatcg acatcactgt aaatatgctt gctgccgggc
    20281 tgatactcta gcggcgtgcg cttgatcatc tccagcgtct ggcctttatc ctgggaatat
    20341 aacgcgcccg cgaccgcttt attcggatat tgtgggtccg ccgggaaacc gccgctgtga
    20401 tgcagcaggt cagaaatccg cagcgtgttt ttgcctttga tggtgtcatt tggactatcg
    20461 gcaaatcccg gaatatattt cgcaatccga tcgtcaggat gcagcttgcc ttcgctcatt
    20521 agcttttgca gagcaaagtt agtggcgtac attttggtat tggaagccag gtcatacagc
    20581 gtcccggtgg tggctttgac aggctgttcc atcaatacgc tgccatcgta ctttttcgcc
    20641 gctccccagg ctttgcgata cacaatctga ttatctttta taatcagcag gtttacgccg
    20701 ggataaccgg cgtccacttg ctggctaatc cagcgatcca tctggttaag ccgttccacg
    20761 ttgaaccctg ctttctctgg cgaactttct gtcagaacag ggtattttgc ggcgctgacg
    20821 ctacaactaa cagccagcag agaaagatag agcattgtcc gtttcatgat atcagtccag
    20881 attgacgtta cggcagccaa agagcgtggt gaaaataaac ccgcaaaccc atgccaccag
    20941 aatcccgcca gcataaaccg ccattgccgg aaggatgcct tgtgaggaag tcatcagcgg
    21001 cagcgccacc agaccagacg gcccaaaggc gctgtttaag cccatcggca gaccccacca
    21061 ggctatcaat ccgataaaca aaccgcccgc cgcgccgcct aaacaggcgg taataaacgg
    21121 tttcatgcgg ggcagggtga cgccataaat cagcggttcg ccaacgccaa gcagaccggg
    21181 aataatcgcc ccgcgtacct gactgcgtaa tgcactgtgc ggttgcgccc gccagtagag
    21241 tgccagcgcc gcgcccacct ggcccgcgcc tgccattgaa aggatgggaa ataagctgtt
    21301 gaatccctgg ctgtccatta acgcgaggta aacaggaata aagccctgat gcacgccaaa
    21361 taccacggcg atcaggaaca gcccggctaa aaccgcacaa ccgagcggat tactgttcag
    21421 gtgcataaac agccacgaca taccttcgaa taaccagccg cccagcggca taatgatcag
    21481 gtaggccagc gtagcggtga tcagtagggt gattaacgag gtcagcagca tgtcgagatc
    21541 gtccggcata aagcggcgta ccatgccttc aatgcgagcg caggcccacg cggcaatcaa
    21601 cacgccgata atattgccgc gcggatcgat gggcagacca aagaaatcgt gaaaaccggc
    21661 gtagtaaccg gtggttgcag cggggttata accgagcaaa aagagtgcgg caataattgc
    21721 gccattgacg cccgtgccgc caaatgcctg ggcggcgtta tagcccacca gaatcaccag
    21781 gaaggtgaac aaacctttgc tgaacacctt cataaaattc agcgcgtcgg gtagcgtgcc
    21841 ctgagcatct gccggaacgt gcatcaccgt ggcaattaac gtcgctatcc ccagcagcag
    21901 accggcggca ataaaaccgg ggataagcgg cgtgaagatg gtggcgaatt tggcgagaaa
    21961 ttgttgcacg ccggaggttt gtttggcttt taactgacgt ttgttctggg cggcgatttc
    22021 tgcggcatcc tgtaccggtg cttctcccag cagctcgctc atcgcttttg cggcacgatg
    22081 cgctttacca ggcccaaaaa ccacctgcac ctgatcgctg gtcaaaatga cgcccttcac
    22141 gccttccagc gttttgatat cgggatcaac cagtgaactg tcatgtacac ccagacgcag
    22201 gcgcgtcata cagttaccac aactggcgat atttcccggt ccgccgacac gggtaagaat
    22261 ggtgttcaga agttcactgc tgatctcttt ggccatgagt tattccttgt ctaaaacctt
    22321 gcgaataaag ccgccgtgtt gatccaggcg ttttttagct tctgcggcat cgagattttt
    22381 cagcaccatc acaatggccg ttttacagtt gcgctcgcag gcaattaacg ccgcttccgc
    22441 ttgctctgcg ctacatccgg tagcgttttt gacgatattg acctggcgca cgtgcagttt
    22501 ttcgttggtg gcgaccacat cgaccatcag gttgccgaac actttgccgg atttaatcat
    22561 cagcccggtg gagagcatat tgagcaccag tttctgcgct gtgcctgctt tcattcgcga
    22621 agaaccagtg accacttcgg cacctacaat cggtgtaatg gcaaactcgg cggtggttga
    22681 aacggcactc cccggattac aggaaattcc cactgtacgg cagccgagct ggcgtgcata
    22741 ttccagtccg gcaatcacat acggcgtgcg accgctggca gcaatgccaa ccaccacatc
    22801 ctgcgccgtt aaattaatat ttttcagatc gttaatgccg ccttcccggc tatcttccgc
    22861 gccttccacc gcgtgctgaa tggcatattc gccgccagca atcaaaccca cgaccagacc
    22921 cggtttcacg ccgtaggtcg gcggacattc gctggcatcc agaatcccca gacgaccgga
    22981 tgttcccgca ccgagataaa tcagacgacc gccgccgctg acctgggcgt gaataacatc
    23041 gatcgccgcg gcgatatccg gcagtacgcg ctcaacggcg agtggtacgg ttttatcttc
    23101 atcgttgata atccggcaca tttccagcgt cgatacgcgg tcaatttcag ccgaagcggc
    23161 gttcgagcct tcggtaatca tcttttcaag ttgcatcggt ggtgacctta ctatttacga
    23221 tcaaataatt gcatatgtat ggaatcatat atcccgtatt gaccgactta aaggaatcat
    23281 tgagtctaca ctcgcagagt gtgaagagcg tcataagaac ttaagcggaa tttttgctgc
    23341 cagcccagga gagtggaaga tgttgtacct gacgaaaata cgcaatgcgg aaagtgaatt
    23401 taccgggaac gagcaaaaaa ttgctgactt tctgcgggcc aacgtgagtg agctgaaatc
    23461 cgtctcttcg cgcaagatgg cgaaacagct tggcattagc cagtcgagta ttgtgaagtt
    23521 tgcccagaag ctcggcgcgc aagggtttac tgaattgcgt atggcgttaa ttggcgaata
    23581 cagcgccagc cgggagaaaa cgaacgctac ggcgctgcac ctgcacagtt cgattaccag
    23641 tgacgattcg ctggaagtga ttgcccgtaa actgaatcgc gaaaagcagc tggcgctgga
    23701 gcaaacctgc gcgttgtttg acttcgcgcg gctgcaaaaa atcattgagg tcatcagtaa
    23761 agcaccgttt atccagataa cgggcctggg cgggtcggcg ctggtggggc gcgatctgtc
    23821 gttcaaactg atgaaaattg gctatcgcgt ggcctgcgaa gccgacacac atgtacaagc
    23881 caccgtttcc caggcgctga aaaaaggcga tgtacaaatc gctatctctt acagcggcag
    23941 taaaaaagag attgtcttgt gcgccgaggc agcacgaaag cagggggcaa cggtgattgc
    24001 cattacctcg ttaaccgatt ccccgctgcg gcggctggcg cattacaccc tcgataccgt
    24061 ttctggcgaa accgaatggc gtagctcgtc gatgtctacc cgcacggcgc aaaattctgt
    24121 taccgattta ctgtttgttg gccttgtgca actcaatgat gtcgagtcat tgaaaatgat
    24181 tcagcgcagc agtgagctaa cacaacgcct gaaatgatgc ataaagcagc gactggattg
    24241 agattttcct gaattagtga gctgaaccgc agcaatattt tgtttatcct gtattttcag
    24301 agggaatgga gtgtaacgct ctgtattaac aaggagagca ttaaaatggg taaactcacg
    24361 ggcaagacag cactgattac gggcgcattg cagggaattg gcgaaggaat tgccagaact
    24421 tttgcgcgtc atggcgcaaa cctaatcttg ctggatatct cccctgagat cgaaaagctg
    24481 gcggacgaac tgtgtggtcg tggtcatcgc tgtacggcgg ttgtcgccga tgtgcgtgac
    24541 ccggtgtcgg tagccgcagc tatcaaacgc gcgaaggaaa aagaagggcg cattgatatc
    24601 ctggtgaata acgcaggcgt ttgtcgtctg ggcagtttcc tcgatatgag cgatgaagat
    24661 cgcgatttcc atattgatat caatattaaa ggcgtatgga acgtcacgaa ggcggtgctg
    24721 ccggagatga ttgcccgcaa agatggtcgc attgtgatga tgtcttcggt cactggcgat
    24781 atggtggccg atccgggtga aacggcgtat gccttaacga aagcggcgat tgttggcctg
    24841 actaaatcgc tggcggtgga gtatgcgcag tctggtattc gcgttaacgc catatgccct
    24901 ggatacgttc gcacaccaat ggcggaaagc attgcccgcc agtcgaaccc ggaagatcca
    24961 gaatcggtgc tgactgaaat ggcgaaagca atcccgatgc gtcgcctcgc cgatccgttg
    25021 gaagttggcg aactggcggc cttcctcgca tcggatgaat ccagctattt aaccggtaca
    25081 cagaatgtga ttgatggcgg cagcacactg ccggagacgg ttagcgtcgg tatctgattc
    25141 acctctgttt cctccctgca tttgtgggga ggatttcgtc ttgaactacg ttcaccaggc
    25201 tattttattt gtcattttgg ccccgggcag tgctcgaaat cctcacgtac tatgtgtacg
    25261 ctccggtttc tccgcgctgt tcgtgtccaa actgactgca acaattacgc ctgttgaacc
    25321 aagttcttat tcccttttca acttccaaat caccaaacgg tatataaaac cgttactcct
    25381 ttcacgtccg ttataaatat gatggctatt agaaagtcat taaatatata agggtgcgca
    25441 atggccgtta acttactgaa aaagaactca ctcgcgctgg tcgcttctct gctgctggcg
    25501 ggccatgtac aggcaacgga actgctgaac agttcttatg acgtctcccg cgagctgttt
    25561 gccgccctga acccgccgtt tgagcaacaa tgggccaaag ataacggcgg cgataaacta
    25621 acgataaaac aatctcatgc cgggtcatca aaacaggcgc tggcgatttt gcagggctta
    25681 aaagccgacg ttgtcactta taaccaggtg accgacgtac aaatcctgca tgacaaaggc
    25741 aagctgatcc cggcggactg gcaaacgcgc ctgccgaata atagctcgcc gttctactcc
    25801 acaatgggct tcctggtgcg taagggcaac ccgaagaata tccacgactg gaacgacctg
    25861 gtgcgctccg atgtgaagct gattttcccg aacccgaaaa cgtcgggtaa cgcgcgttac
    25921 acctatttag ccgcgtgggg cgcagcggac aaagccgacg gtggcgataa agccaaaacc
    25981 gaacagttta tgactcagtt cctgaaaaac gttgaagtgt tcgataccgg cggtcgtggc
    26041 gcgaccacca cttttgccga gcgcggcctg ggtgatgtgc tgatcagctt cgagtcggaa
    26101 gtgaacaaca tccgtaaaca gtatgaagcg cagggttttg aagtggtgat tccgaaaacc
    26161 aacattctgg cggaattccc ggtggcatgg gttgataaaa acgtgcaggc caacggtacg
    26221 gaaaaagccg caaaagccta cctgaactgg ctctacagcc cgcaggcgca aaccatcatc
    26281 accgactatt actaccgcgt gaataacccg gaagtcatgg acaaactgaa agacaaattc
    26341 ccgcagaccg agttgttccg cgtggaagac aagtttggct cctggccgga agtgatgaaa
    26401 acccacttca ccagcggcgg cgagttagac aagctgttag cggcggggcg taactgatgt
    26461 ttgctgtctc ttccagacgt gtgctgccgg gctttacctt aagcctcggc accagtctgc
    26521 tgtttgtgtg cctgattttg ctgctgccgc tctccgcgct ggtgatgcaa ctggctcaga
    26581 tgagctgggc gcagtactgg gaggtgatca ccaacccgca ggtggtcgcg gcctacaaag
    26641 tgacgctgct gtcagcgttt gtggcatcga tttttaacgg cgtttttggc ctgctgatgg
    26701 cgtggatttt aacccgttat cgcttccctg gccgcacgct gcttgatgcg ctgatggatt
    26761 taccctttgc gctgccaacg gcagtcgctg gtttaacgct ggcctcgctc ttttccgtaa
    26821 acggttttta cggtgaatgg ctggcgaagt ttgatatcaa agtcacctac acctggcttg
    26881 ggattgcggt ggcgatggcc tttaccagca ttccgtttgt ggtgcgtacc gtgcagccgg
    26941 tgctggaaga gttaggcccg gaatatgaag aagcggcgga aacgctcggt gccacgcgct
    27001 ggcagagttt ctgcaaagtg gtgttaccgg agctttctcc ggcgctggtg gcgggtgtgg
    27061 cgctgtcgtt tacccgcagc ctcggtgagt ttggcgcggt gatttttatc gccgggaata
    27121 tcgcctggaa gacagaagtg acgtcgctga tgatctttgt gcgcttgcag gagtttgatt
    27181 acccggcggc gagcgcgatt gcttcggtga tcctcgcggc atccctgctg ctgctgttct
    27241 caattaacac tctgcaaagt cgctttggtc ggcgtgtggt aggtcattaa tggcggaagt
    27301 gacacagttg aagcgttatg acgcgcgccc gattaactgg ggcaaatggt ttctgattgg
    27361 catcgggatg ctggtttcgg cgttcatctt gctggtgccg atgatttaca tcttcgtgca
    27421 ggcattcagc aaggggctga tgccggtttt acagaatctg gccgatccgg acatgctgca
    27481 cgccatctgg ctgacggtga tgattgcgtt gattgccgtg ccggtaaatc tggtgttcgg
    27541 cattctgctg gcctggctgg tgacgcgctt taacttcccc ggacgccagt tactgctgac
    27601 gctactggac attccttttg ccgtgtcacc ggtggttgcc ggtctggtgt atctgctgtt
    27661 ctacggctcc aacggcccgc tcggcggctg gttggatgaa cataatctgc aaattatgtt
    27721 ctcctggccc ggaatggtgc tggtcactat cttcgtgacg tgtccgtttg tggtgcgcga
    27781 actggtgccg gtgatgttaa gccagggcag tcaggaagac gaagcggcga ttttgcttgg
    27841 cgcatccggc tggcagatgt tccgccgtgt cacattgccg aacatccgct gggcgctgct
    27901 ttatggcgta gtgctgacca acgcccgcgc gattggcgag tttggcgcgg tgtcggtggt
    27961 ttccggctcg attcgtggcg aaacactttc gctgccgtta cagattgaat tgctggagca
    28021 ggactacaac acagtcggct cctttaccgc cgccgcgctg ttgacgctga tggcgattat
    28081 caccctgttt ttaaagagta tgttgcagtg gcgcctggag aatcaggaaa aacgcgcgca
    28141 gcaggaggaa aatcatgagc attgagattg ccaatattaa gaagtcgttt ggtcgcaccc
    28201 aggtgctgaa cgatatctca ctggatattc cttcaggtca gatggtcgcg ttgctggggc
    28261 cgtccggttc cgggaaaacc acgctgctgc gcattatcgc tgggctggag catcaaacca
    28321 gcgggcatat tcgcttccac ggcaccgacg tgagccgcct gcacgcacgt gatcgtaaag
    28381 tcggtttcgt gttccagcat tacgcgctgt tccgccatat gacggtgttc gacaatatcg
    28441 cttttggcct gacggtgctg ccgcgtcgcg agcgcccgaa tgccgcggcg atcaaagcga
    28501 aagtgacaaa attgctggag atggtgcagc ttgctcatct ggcggatcgt tatccggcgc
    28561 agctttccgg cggccagaaa cagcgtgtgg cgctggcgcg tgcgctggct gtggaaccgc
    28621 aaattctgct gcttgatgaa ccgtttggcg cgctggatgc gcaggtgcgt aaagagctgc
    28681 gtcgctggct gcgtcaactc catgaagaac taaaattcac cagcgttttt gtgacccacg
    28741 atcaggaaga agcgaccgaa gtagctgatc gtgtagttgt gatgagccag ggcaatattg
    28801 aacaggctga cgcgccggat caggtatggc gcgaaccggc gacccgtttt gtgctcgaat
    28861 ttatgggcga agtgaaccgc ctgcagggaa ccattcgcgg cgggcagttc catgttggcg
    28921 cgcatcgctg gccgctgggc tacacacctg cgtatcaggg gccggtggat ctcttcctgc
    28981 gcccttggga agtggatatc agccgccgta ccagcctcga ttcgccgctg ccggtacagg
    29041 tactggaagc cagcccgaaa ggtcactaca cccaattagt ggtgcagccg ctggggtggt
    29101 acaacgaacc gctgacggtc gtgatgcatg gcgacaatgc cccgcagcgt ggcgagcgtt
    29161 tattcgttgg tctgcaacat gcgcggctgt ataacggcga cgagcgtatc gaaacccgag
    29221 atgaggaact tgctctcgca caaagcgcct gataggttga gtgaatgtta aacgcccgga
    29281 ggcgcttccc gcgatccggg ctttttaatg gtgtaggcct gataagacgc gcaagcgtcg
    29341 catcaggcaa caccacgtat ggatagagat cgtgagtaca ttagaacaaa caataggcaa
    29401 tacgcctctg gtgaagttgc agcgaatggg gccgaataac ggcagtgaag tgtggttaaa
    29461 actggaaggc aataacccgg caggttcggt aaaagatcgt gcggcgcttt cgatgatcgt
    29521 cgaggcggaa aagcgcgggg aaattaaacc gggtgatgtg ttaatcgaag ccaccagtgg
    29581 taacaccggc attgcgctgg caatgattgc cgcgcttaaa ggctatcgca tgaaattgct
    29641 gatgcccgac aacatgagcc aggaacgccg tgcggcgatg cgtgcttatg gtgcggaact
    29701 gattctggtc accaaagagc agggcatgga aggtgcgcgc gatctggcgc tggagatggc
    29761 ggatcgtggt gaaggaaagc tgctcgatca gttcaataat cccgataacc cttacgccca
    29821 ttacaccact accgggccgg aaatctggca gcagactggc gggcgcatta cccattttgt
    29881 ctccagcatg ggaacaaccg gcactatcac cggcgtgtca cgctttatgc gcgaacaatc
    29941 caaaccggtg accattgtcg gcctgcaacc ggaagagggc agcagcattc ccggcattcg
    30001 ccgctggcct acggaatatc tgccggggat tttcaacgct tctctggtgg atgaggtgct
    30061 ggatattcat cagcgcgatg cggaaaacac catgcgcgaa ctggcggtgc gggaaggaat
    30121 attctgtggc gtcagctccg gcggcgcggt tgccggagca ctgcgggtgg caaaagctaa
    30181 ccctggtgcg gtggtggtag cgatcatctg cgatcgtggc gatcgctacc tttccaccgg
    30241 ggtgtttggg gaagagcatt ttagtcagag ggcggggatt taagattatt gcgcaggcac
    30301 tgttttatcc gtctgcgcaa ttaacgcatg taaaaaaggc tgtgcgttct catcgctctt
    30361 accggggatc ttcacaatat acggcttccc ggtaatcgat gacgacgaag caaccttatc
    30421 aataaactgc tcggcagtgt caatgcggtt acgggtattg cccagcttca gacgcagatg
    30481 agaaaccgct tcatcgcagg tatgttcatc accgttgcgc acaaatatca aatccttttt
    30541 ctgtgctaat ccctccagca tggcgttgat gcgggcttct tcatgggcag ttaacttcgc
    30601 gtaagcgggg agtgtcatta gtagtgtaat gaccagacaa atgattttct tcactgaatt
    30661 accttatcct tgttttgtaa tagccgattt gactgccaga atcaggtaaa gtttcggttg
    30721 gaatacgatg aggatcatcg gcaaggagaa aaaattaaat gagtagtttg ttgttgttta
    30781 acgataagag cagggcactg caggcggata tcgtcgccgt gcagtcgcag gtagtgtacg
    30841 gcagcgtagg caacagcatt gccgtgcctg ctatcaaaca gaacggcctg aatgtctttg
    30901 ccgtgccgac ggtattgctg agcaatacgc cgcattatga cactttctac ggtggtgcga
    30961 ttccggacga atggtttagc ggctatttgc gtgcgcttca ggagcgtgat gcgctgcgcc
    31021 aacttcgagc tgtaaccacg ggctatatgg gaacggcatc gcaaatcaaa atccttgccg
    31081 agtggctgac tgcgctacgc aaagaccatc ctgacctatt gatcatggtc gatccggtga
    31141 ttggcgatat tgatagcgga atttatgtca aacctgacct tcccgaggcg tatcgacaat
    31201 atttactgcc gctggcgcag ggaattaccc ccaatatctt tgagctggaa atcctgaccg
    31261 gtaaaaattg ccgcgatctc gacagtgcca ttgctgccgc aaaaagtctg ctttcagaca
    31321 cactaaaatg ggtggtgatt accagcgcct ccggtaatga agaaaatcag gagatgcagg
    31381 ttgtggtggt cactgccgac agcgtgaatg tcatttccca ttcacgggta aaaaccgacc
    31441 tgaaagggac tggcgacctg ttttgtgctc agctcatcag tggcttgctg aaagggaagg
    31501 cgttaaccga cgcggtgcac cgcgcgggtt tgcgtgtact ggaagtgatg cgctacaccc
    31561 agcaacatga gagcgatgaa ttgattttgc caccgctggt ggaagcataa aaaaatggcg
    31621 ccaatcggcg ccatttttca ctgcggcaag aattacttct tgatgcggat aaccggggtt
    31681 tcacccacgg ttacgctacc ggacagtttg atcagttctt tgatttcgtc catgttggag
    31741 ataacaaccg gagtcagggt agacttggct ttctcttcca gcagcggcag atcaaattca
    31801 atgacagtat cgccaacttt cacgcgctga ccttcttcag caatacgctt gaagccttcg
    31861 cctttcagtt caacggtgtc gataccgaag tggacgaaca gttcaacgcc gctatcagat
    31921 tcgatagaga atgcgtggtt ggtttcaaag attttaccaa tggtgccgtc tacaggcgcg
    31981 accattttgt tacccgttgg tttgatagca ataccatcac caacgatttt ttccgcaaaa
    32041 acgacatccg gcacgtcttc gatattgacg atctcgccag agagcggagc aatgatctca
    32101 atagttccgg tatccttctt gtcgtcggaa accagagatt tcagtttatc gaacaaaccc
    32161 atgatcttct cctaagcagt aaattgggcc gcatctcgtg gattagcaga ttgttttttc
    32221 ttcaatgaac ttgttaacca gcgtcattaa ctcgtccgtt gtcggttgag caagagcctg
    32281 ctctgctaac accttcgcat cttcgaagtt cgtgttacgg ataatcttct taatgcgcgg
    32341 gatagaaatg gcgctcatag agaattcgtc cagacccatc cccagcaaca gaagtgtagc
    32401 acgttcatcg ccagcaagct caccacacat gccagtccat ttgccttcag catgagaagc
    32461 atcaataact tgcttgatca agttcagcac ggacggtgac attggctggt aaaggtgtga
    32521 aatcatatca ttaccacggt caactgccag agtgtactgc gttaaatcat tggtgccgat
    32581 actaaagaaa tcaacttctt tggctaaatg acgtgcaatt gttgcggcag ccggtgtttc
    32641 caccattacg ccgatttcaa ttgactcgtc aaacgcttta ccttcgtcgc gcagttcctg
    32701 tttgtagatt tcgatctctt tgcgcagtgc acgcacttct tcaacagaga tgatcatcgg
    32761 gaacataatg cgcaatttac cgaaagccga ggcacgcagg atagcgcgga gctgatcgcg
    32821 caggatctct ttacgatcca tcgcgatacg gatagcgcgc cagccgagga acgggttctc
    32881 ttctttcggg aagttcatgt atggcagctc tttgtcgccg ccgatgtcca tggtacgaac
    32941 gataaccgcc tgcgagccac acgcttcagc cactgctttg taagcagcaa actgttcttc
    33001 ttcagtgggc agcgcgtcgc ggtccatgaa caggaactca gtacgataca gaccaacgcc
    33061 ttcagcgccg ttacgctctg caccttcaac gtcacgaacc gtaccaatgt tagcgcatac
    33121 ttctacctgg tgaccgtcca gcgtaatagc tggcagatct ttcagtttag caagctctgc
    33181 tttttcagaa gccacttgct cctgaacagc gcgcatttta tcaataactt cgttggttgg
    33241 attgacgtaa acctgattat ttacggcatc cagaatcaga tagtcgtcat ttttcacctg
    33301 agaggtgacg ctaccggtac ccacgatagc aggcagttcc agagaacgcg ccatgataga
    33361 ggtgtgggaa gtacggccac ccgcgtcggt gatgaaaccc agcaccttct tcaggttcag
    33421 ctgtgcggtt tcggacggcg tcaggtcagc ggcaaccaga atgacttcat cctgaatggc
    33481 gctcaggtcg ataatcttca ggcccaggat gttgcgcagc aggcgcttac cgatatcacg
    33541 tacgtcagcc gcacgttctt tcaggtattc atcatccagc tcttccaggg cagaagcctg
    33601 accttcgata acttcatgag cagctgcgtc agctgtcatg tgcttatctt taatcagggc
    33661 tatgatttcc tgctccagct cctcatcttc gagcagcata atatgccctt caaagatggc
    33721 ttctttttct tcaccgaacg tttcaccagc tttcgttttg atcgtttcca gctgggctga
    33781 tgccttggca cgaccgctca gaaaacgttc aacttcctga tcaacctggt cggcagaaat
    33841 ttttttccgg tcaatgacaa tttcgtcttc tttcagaagc agagctttac cgaaagcgat
    33901 acccggggat gctaaaatgc ctgaaatcat aaccctacct tacttgtgac tgatttttaa
    33961 aagaacccgg gaaattactc gagttccgcc atcagtttaa ccagatgttc aaccgctttc
    34021 tgctcgtctt cgccttctgc ggagatagtc acaacggtac cttgagtcag gcccagagtc
    34081 tgcagtttaa acaggctttt cgcgctggcg cttttgccgt tggaagtcac agtaatttca
    34141 gaagtgaagc ccttagcttc ttttacaaac tgggcagcag ggcgggtgtg cagaccgttc
    34201 ggagcggtaa tggtaacttc ttgctggaac attgtatttc cccaacttat aggtttagtg
    34261 ttgtggaact aaagtctagc ctggcggttc gactttagcc tgtattgtta gcgccagttt
    34321 ttaacagacg cgacgcacga agagcgcaat acagtccatt gctggcgggt gttgaccgct
    34381 gacgtttcgt acgtcattaa acattatgcc gctaaaggaa gaattgaacc aaatcataaa
    34441 atcgattcag ctttggcttt tcctgtaacg attaatttcg cgcatcaaaa taattagtct
    34501 ggttaaatac cagttccgac aggctgaatc aatgccagga ggggcgaaag tttgaagcag
    34561 gccacaaaaa agcacctaaa aaggtgcttt tttacgcatt tttaacaagc tggcattact
    34621 gttgcaattc tttctcagtg aagagatcgg caaacaatgc ggtgcttaaa taacgctcac
    34681 ccgatgacgg tagaataacc acaatattct tgttggtaaa gctttcatct tcttgtagtt
    34741 tcaacgccgc ggcaacagct gctccagaag agatacctgc aagaatacct tcttcttcca
    34801 tcagacgacg ggcggtagaa atcgcttctt cattggtgat gccaatgact ttatcgacca
    34861 gcttgagatc gaggttagcc gggataaaac cagcgccaat accctgaatt ttatgcgggc
    34921 caggtttaat ctcttcacct gccagcgcct gggcgataac tggagaatcg gttggctcaa
    34981 cggcgacaga gataagatcg gtcttacctt tggtgccttt aatgtagcgg ctgacgccag
    35041 tcagcgtacc gccagtccca acgccagcaa taaatatatc aacctgaccg tcggtatctt
    35101 cccagatctc cggaccggtg gttttttcgt gaatttcagg gtttgccgga ttgctgaatt
    35161 gttgcagcag caggtatttc tctggattgc tggcgacaat ttcttctgct ttttggattg
    35221 cgcctttcat gcctttagca ccttcggtca gcaccaggtt tgcacctaac gctttcagca
    35281 gcttgcggcg ttcaatactc atggtttctg gcatggtcag ggtgagtttg taaccgcgag
    35341 cggcagctac ataggccagt gcaatcccgg tattaccgct ggttggttca acaagttcaa
    35401 cgcctggttt cagcacgccg cgcttttcgg catcccaaat catgttggca ccgatacggc
    35461 acttaacgct gaagctgggg ttacgagatt ccaccttcgc cagaatgcgt ccgttaccga
    35521 tgcgattcag gcgaaccagc ggcgtgtgac cgatagtcag cgagttatct tcaaaaatct
    35581 tactcatggc ctgtccttaa ctgtatgaaa ttgggataca acaggtagca tacccgctca
    35641 gagaatatgc ggaagtaagg atttagcata tctatatgca gaagggaaat aatgacatgc
    35701 aagatggaat aaggggcggc ataagccacc cctgtttcac acaaacggtt tacaaaataa
    35761 ccggtagatt gttaccgcca catcgcgtgt ttatcgcgat agcaatcgac ccacatcgcc
    35821 gtcgcgccac aaacggcaac gggcatgatg aacagattaa gcagcgggat catcgtaaac
    35881 aggctggtta aagcaccaaa ctgcatattg gtgattttgc gtgtgcgcag ggcggtgcgc
    35941 atctctttaa acggcacttt gtggttatcg aaggggtaat cgcaatactg gatggctaac
    36001 atccaggcgc taaacaggaa ccacagtacc ggcgcgacgg tttgcccaat accagggatg
    36061 aagtaaagaa ttagtaatac aattgcgcgc ggcagatacc aggcaaattt ttgccattcg
    36121 cgtttcatga ttcgcggcac atctttcatg ataccgaaaa tcccggtatc tggcggtgta
    36181 gcgccagtca atcgtgcttc cagttgttca gccaataaac cgttaaacgg agcggcaatc
    36241 cagttagcaa tcgtggagaa gaaatagcca aacactaaca gcacagagat gacagccaga
    36301 ggccacaaca gatagctcag ccattgtaac cagtccggaa cataactcat gagagtcggg
    36361 atccagacat cgagttgcgt aaagagccac cagaatgcgc cccccatcaa caaaatattg
    36421 accagcagcg gtaaaataac gaaacgccga atcccaggtt gcgagacgag cttccagcct
    36481 tgcgcaaaat agtaaaaacc gctgcgtggg gcagatgtga atgatgaaac cataatcagg
    36541 atgagctcct tttgaccaat cccaggaaaa ttgtgcgtat tttaccgggt aattgcgcaa
    36601 tggacagtta ggatatgttc gaaaaaacag caaaaagcac gatttcatct atctttgtgc
    36661 tgtgaaagtt aatagtgcac ttgcacttga ggtaatcggc aaatactctt agtgagtaaa
    36721 tgtttgccgt ggtggcaagg tgttagaaca acagagaata taatgatgca ggatttgcgt
    36781 ctgatattaa tcattgttgg cgcgatcgcc ataatcgctt tactggtaca tggtttctgg
    36841 accagccgta aagaacgatc ttctatgttc cgcgatcggc cattaaaacg aatgaagtca
    36901 aaacgtgacg acgattctta tgacgaggat gtcgaagatg atgagggcgt tggtgaggtt
    36961 cgtgttcacc gcgtgaatca tgccccggct aacgcccagg agcatgaggc tgctcgtccg
    37021 tcgccgcaac accagtacca accgccttat gcgtctgcgc agccgcgtca accggtccag
    37081 cagccgcctg aagcgcaggt accgccgcaa catgctccgc gtccagcgca gccggtgcag
    37141 caacccgtgc agcagcctgc ctatcagccg cagcctgaac agccgttgca gcagccagtt
    37201 tcgccacagg tcgcgccagc gccgcagcca gtgcattcag caccgcaacc ggcacaacag
    37261 gctttccagc ctgcagaacc cgtagcggca ccacagcctg agcctgtagc ggaaccggct
    37321 ccagttatgg ataaaccgaa gcgcaaagaa gcggtgatta tcatgaacgt cgcggcgcat
    37381 cacggtagcg agctaaacgg tgaactgctt cttaacagca ttcaacaagc gggcttcatt
    37441 tttggcgata tgaatattta ccatcgtcat cttagcccgg atggcagcgg cccggcgtta
    37501 tttagcctgg cgaatatggt gaaaccggga acctttgatc ctgaaatgaa ggatttcact
    37561 actccgggtg tcactatctt tatgcaggta ccgtcttacg gtgacgagct gcagaacttc
    37621 aagctgatgc tgcaatctgc gcagcatatt gccgatgaag tgggcggtgt cgtgcttgac
    37681 gatcagcgcc gtatgatgac tccgcagaaa ttgcgtgagt accaggacat catccgcgaa
    37741 gttaaagacg ccaacgcctg atacacttaa ggcaaattaa ctcctcttcg aacccccgct
    37801 tgtcgggggt ttttagcatt gatggtgcga tatggaatca atcgaacaac aactgacaga
    37861 actgcgaacg acgcttcgcc atcatgaata tctttatcat gtgatggatg cgccggaaat
    37921 tcccgacgct gaatacgaca ggctgatgcg cgaactgcgc gagctggaaa ccaaacatcc
    37981 agaactgatt acgcctgatt cgcctaccca acgtgtaggt gctgcgccgc tggcggcttt
    38041 cagccagatt cgccatgaag tgccaatgct gtcgctggat aacgtttttg atgaagaaag
    38101 ctttcttgcc ttcaacaaac gagtgcagga ccgtctgaaa agcaacgaga aagtcacctg
    38161 gtgctgtgag ctgaagctgg atggccttgc tgtcagtatt ctgtatgaaa atggcgtttt
    38221 agtcagtgcc gcgacccgtg gcgatggcac caccggggaa gatatcacgt ctaacgtgcg
    38281 taccattcgc gccattccac tgaagctgca cggagagaat atcccggcgc gtctggaagt
    38341 gcgtggtgaa gtgttcctgc cgcaggcggg gttcgaaaag attaacgaag atgcgcgacg
    38401 cacgggcggg aaagtttttg ctaacccacg taatgcggca gctggttcac tgcgtcagct
    38461 tgatccgcgt attacagcga agcgaccgct cacttttttc tgctatggcg ttggtgttct
    38521 ggaaggtggc gagctgccgg atactcatct tggccgttta ctgcaattta aaaagtgggg
    38581 gttgccggtc agcgatcggg taacgctttg tgaatcggcg gaagaagtgc tggcgttcta
    38641 tcacaaagtg gaagaagacc gcccgacgct gggctttgat atcgacggcg tggtgattaa
    38701 ggtcaactca ctggcacagc aggagcagct tggctttgtc gcgcgagccc cgcgctgggc
    38761 ggtagcgttt aaattcccgg cgcaggaaca gatgaccttt gtgcgtgacg tcgagtttca
    38821 ggttgggcgt actggcgcga ttacgcctgt tgcgcgtctg gaacctgtcc atgttgcagg
    38881 cgtgctggtg agtaacgcaa ccttacacaa tgcggatgaa atcgaacgtc ttggtttacg
    38941 cattggcgat aaagtggtga ttcgccgcgc tggcgacgtg atcccgcagg tggtgaacgt
    39001 cgtgctttct gaacgcccgg aagatactcg tgaggttgta ttcccgacgc attgtccggt
    39061 atgtggttct gacgttgagc gtgtggaagg tgaagcggtt gcccgctgta ccggtggcct
    39121 gatttgcggt gcgcagcgta aagagtcgct gaaacacttt gtttcccgcc gtgcgatgga
    39181 tgttgacgga atgggcgaca aaatcatcga tcagctggtt gaaaaagaat atgtccacac
    39241 tccggcggat ctgttcaaac tcaccgcagg caaacttacc ggactggagc gtatggggcc
    39301 aaaatcggca caaaacgtgg ttaacgcgct ggaaaaagcg aaagaaacca cctttgctcg
    39361 cttcctctat gcacttggca tccgtgaagt cggcgaggcc accgcagcag gtctggcggc
    39421 atatttcggc acgctggaag cgctggaagc cgcttcgatt gaagagctgc aaaaggtgcc
    39481 tgatgttggc attgtcgttg catcccacgt tcacaacttc tttgccgaag aaagcaaccg
    39541 caatgtcatc agcgagctgt tggcggaagg tgttcactgg cctgcgccga tcgttatcaa
    39601 cgcggaagag attgacagcc cgtttgctgg taaaaccgtg gtgcttacgg gcagcttaag
    39661 ccagatgtcg cgtgatgacg ctaaagctcg actggtcgaa ctgggcgcga aagtcgcggg
    39721 cagcgtgtcg aagaaaaccg atctggtgat agcgggtgaa gctgcaggat ctaaactggc
    39781 gaaggcgcag gaactgggca ttgaagtcat cgacgaagcg gaaatgctgc gtttgctggg
    39841 tagctgagat ggaaaaagag cagctgattg aaatagccaa tacgataatg ccgtttggca
    39901 aatacaaagg gcgtcgctta atcgacctgc cggaggagta tctgctgtgg tttgcccgca
    39961 aagatgagtt cccggcaggg aagctcggtg agctaatgca aatcacgctg ctgattaaaa
    40021 ccgaggggct gacgcaactg gtccagcccc tgaagcgtcc gctttaagct ttatcggtgc
    40081 tgctttcctg ctgcgcctgt aactgttcgg tctggcgttt gtatcgacgc gccagcaccg
    40141 cacagaccat caattggatc tgatggaaaa tcatcagggg cagcaccatc ataccgatca
    40201 ccgatgtggg gaacagaatg tttgccatcg ggatgccatt tgccagactc tttttcgaac
    40261 cacaaaagac gatagtaatt tcatctgcct tattgaagcc cagtcggcgt gccataaaga
    40321 cgttaactac aatcacgata gccagaagaa cgcagctgac caccacgata aacagcaatg
    40381 atccccagcc aactttatgc cagataccat taacgacggc ttcgctgaac gctgtataaa
    40441 ccaccaacag aatggacgtc tggtcagttt tcgcaatcca ttttttattg cgcgacaccc
    40501 agtcaccaat ccacggccgg gaaagatgcc ccaacacaaa cggcagcagc agttgcagca
    40561 taattttacc gacctgctca aggctgcccc ctgcaccgtg aacattcatc accagaccaa
    40621 ccagcaatgg tgaaaggaaa atccccagca ggctggatgc tgacgcagaa caaaccgccg
    40681 ccgcgacgtt accgcccgcc attgacgtga aggcgattgc agactgcacg gtagccggga
    40741 gaatgcacaa gtagagaaaa ccggagtaga gcatcgggtc gacatttacc ggtttccacc
    40801 aggcaaacag tacacccaga atcggaaaca gcacgaaggt gctgcacatt acccacaaat
    40861 gcagtcgcca gtgaccaccg ccagcaataa tcgcctcacg cgacaacttc gcgccgtgca
    40921 taaagaacag cagggcaata gctgcggtgg tcagattttc aaagaagggg acgaaatcgc
    40981 ctctggccgg aaagaaagag gccagcaaca ccaccgtgat cagggttaag gtgaaaggat
    41041 cgaggatacg aaaaagtttc ataaagactc cagatgatcg atggttctat tgtgcttctt
    41101 ccagtttgag aaataaaatt gatttattgc atccattcat gaagaaaaca gatgaattat
    41161 tctttaaaac aattaaaggt tttcgtcaca gtagcgcagg agaaaagttt tagtcgtgca
    41221 ggagagcgta tcggcctgag ccagtcggca gtaagtcaca gtgtgaagga gctggaaaat
    41281 catactggtg ttcgcctgct ggacagaacc acgcgtgaag tggtgcttac agatgcaggg
    41341 cagcagttgg ctttgcgtct tgagcgactg ctggatgaac ttaacagcac gttgcgcgat
    41401 accgggcgta tggggcaaca actgagcgga aaagttcggg tcgctgccag ccagaccatt
    41461 tccgcgcatc ttattccgca atgcattgct gaaagccatc gccgctatcc agatattcag
    41521 tttgtcctgc acgatcgtcc gcagcagtgg gtgatggaaa gtattcgcca gggagatgtc
    41581 gactttggca tcgtcatcga tccaggccca gtgggcgatc tgcaatgtga agcgattctt
    41641 tccgagcctt tctttctgct ttgccatcgg gatagcgctt tggccgcgga agattatgta
    41701 ccctggcagg cattacaggg agcgaaattg gtgttgcagg attacgcgtc aggcagccga
    41761 ccgctgattg acgcagcgct ggcgcgcaat ggtattcagg cgaatattgt gcaggagatt
    41821 ggacatccgg cgacgctatt cccgatggta gccgcaggca tcggcatcag tattcttccc
    41881 gcactggcgc taccattgcc tgaaggtagc ccgctggtgg taaagcgcat tacgccggtt
    41941 gttgaacgcc agttgatgct ggtgcgcagg aagaaccgtt cactttccac cgccgctgaa
    42001 gcgttatggg acgtggtacg agaccagggc aatgcgctaa tggctgggcg ggaaggggat
    42061 ccgttgtatc agatatagat atcgatatga gtcgtttttt caacaggatt tagaagggat
    42121 acattcagag acaaacgctg ctctttttcc tgcgcctttt ctgcctgctg tctctgcaac
    42181 tgcgccagtt gtgtttccag tagggtgatt tgctgttgaa tcaattcggc ctgtttttgt
    42241 ttgtcttcag cactgccgct agtgtctaca atctctttaa tttgttgagt gagtttgatg
    42301 atttgctgac ttatccgcgc gatttgtgaa gcaacatcgc taccttgtga tgccctgtta
    42361 ctactctgtg tttgtatgga aggaattgaa gttgaagaga tagtggccat tgttcagtcc
    42421 tcgtcactgt acgaggtaat atcggtagaa gaacaaggaa cttgagaaga aaaatcaatc
    42481 ttacaaatcg cagatagcaa aaaagcgcct ttagggcgct tttttacatt ggtgggtcgt
    42541 gcaggatgac tcgcttcgct cgcccttcgg gccgtcgccg cgagcggctc cgttgtttcg
    42601 ctgcgctcaa ctcgaacctg ctgcaggttc gaatctttga gtatcgcaga tagcaaaaaa
    42661 gcgcctttag ggcgcttttt tacattggtg ggtcgtgcag gattcgaacc tgcgaccaat
    42721 tgattaaaag tcaactgctc taccaactga gctaacgacc cgaagtggtg ggtgatgacg
    42781 ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc
    42841 cacttcggta cttcacattt ttacaagaaa tttagctggt gagaaagtgg tgggtgatga
    42901 cgggatcgaa ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca
    42961 cccacttctc aatttcttgc tacacggcgg agactacata tagtagttgg tgggtgatga
    43021 cgggatcgaa ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca
    43081 cccccgctgt gtggagtcgc attataggga gagttcaaaa tgagtcaacg cattttctaa
    43141 agaaattatt cgttcgtcgt aaatttaagc aagatgatcg caaaacagac cgtgttgcgc
    43201 aatttgtcaa cgaaaacaat aatgcgtaag gttgcaaccc gaactacatt gaggaatcag
    43261 gcgggagtga tagaatatcg cccactcaat ttttccagga tttgccggtt gtcggcatct
    43321 ttctaaacgt aaggccattt catgaaaatc aaaactcgct tcgcgccaag cccaacaggc
    43381 tatctgcacg ttggcggcgc gcgtactgct ctttactcct ggctttttgc acgtaaccac
    43441 ggcggtgagt tcgtgctgcg tattgaagac accgatcttg agcgttccac gccggaagct
    43501 atcgaagcca ttatggatgg catgaactgg ctgagcctgg agtgggatga aggtccgtac
    43561 taccagacca aacgttttga tcgctacaac gcggtgatcg atcagatgct ggaagagggc
    43621 acagcttata aatgctattg ctctaaagag cgcctggaag cgctgcgcga agagcaaatg
    43681 gcgaaaggtg agaagccgcg ttatgacggt cgctgccgcc acagtcatga gcatcacgct
    43741 gatgatgagc cgtgtgtcgt acgttttgct aacccgcagg aaggttctgt tgtttttgac
    43801 gatcagatcc gtggtccgat cgagttcagc aaccaggaac tggacgatct tattatccgc
    43861 cgtaccgatg gttccccaac ctataacttc tgtgtggttg tcgatgactg ggatatggaa
    43921 atcacccacg ttatccgtgg cgaagaccat atcaacaaca cgccacgcca gatcaacatt
    43981 cttaaagccc tgaaagcgcc ggtaccggtt tacgcgcacg tttctatgat caacggcgat
    44041 gacggcaaaa aactgtccaa acgtcacggg gcagtcagcg taatgcagta tcgtgatgac
    44101 ggttatttgc cagaagcact gctgaactat ctggtgcgtc tgggctggtc ccacggcgat
    44161 caggaaatct tcactcgtga agagatgatc aaatacttca ctttgaatgc cgtcagcaaa
    44221 tctgccagtg cgttcaacac cgacaagctg ctgtggctga accatcacta cattaacgcg
    44281 ctgccgccgg agtatgttgc tactcactta cagtggcaca ttgagcagga aaatatcgat
    44341 acccgtaacg gcccgcagct ggctgatctg gtgaaactgc tgggcgaacg ctgcaagacg
    44401 ctgaaagaga tggcacagag ctgccgttat ttctacgaag attttgctga gttcgatgcc
    44461 gatgccgcga aaaaacatct gcgtccggta gcgcgtcagc cgctggaagt agttcgtgac
    44521 aaactggccg cgattactga ctggacagct gaaaacgttc atcacgctat tcaggcgacg
    44581 gcggatgagc tggaagtggg tatgggtaaa gttggtatgc cgctgcgtgt cgccgtaacc
    44641 ggtgcggggc agtctccggc actggatgtt accgttcacg caatcggtaa gacacgtagc
    44701 attgagcgta tcaacaaagc gctggccttt attgctgaac gcgaaaacca gcagtaatta
    44761 gcgtgtaaaa agataaacgg caggagataa tatcctgccg ttttttattt atgctgattc
    44821 gtcaatatcg agacgggcga ggaaattgcg aattccttca cgtgacaaaa attgcgcgac
    44881 tttttcctgc tcaatgtctg tcatattatc tatcgcgttg atgatttgtc gatacgccgg
    44941 ggcgcgtgct ccaggagcgt attcagcaat tctctcttca gcttccatca acattggtgt
    45001 gttatggaac gcaggcgtgt tctgaataaa ctcgcaaact tgcgtatcga ccagaataag
    45061 tctggcacga ccgcctttca cgccgggaaa tttttccgtt ttccagcctt tttctctgat
    45121 ccaacggtta acagtttgtt tagccacacc gaggcattca gccagttctt cggtggtcat
    45181 tttattgcgt aatcttttca tattatttgc tatcgcgaat ccctaagcgt tgtaacaatc
    45241 cgataatccc ttcccgcaga agcaaggatg taaactgttt ttgttcaact ggcgtcatct
    45301 ctttcgccag tgtcaccagc aatacttcaa gcggcgcatc accagaaagg gcaggcgctt
    45361 ctccctgacc ttctggacgt tcagcattac gaatatattc acgaacctgt tcattgacgt
    45421 gaaccagtcg ggctttgcca ccctggacgc ctggttttgg tgacgtcgtc cagccttcct
    45481 tgcgtaccca tttattaatg gtctggcggc tatagccggt cagtctggca agttcatctg
    45541 gcgtcatcct ctccttgaac ataacaattc cctgaatgta tgtgggtcta ttagtagttc
    45601 attttatagc accactttac aggaaaccgt gacatgaata actactgaat gcgagcggtt
    45661 ttccaaaatt gttcatttgc ctgatttttc agcgattgaa actacacgct gcaatttgcc
    45721 gttgacacat tcgggcggaa ttcatatgat gccgcccgtc aactcgacaa gctttacgtg
    45781 acggggctat agctcagctg ggagagcgct tgcatggcat gcaagaggtc agcggttcga
    45841 tcccgcttag ctccaccaaa ttttgcaccc agcaaatttg gtacgtaaac gcatcgtggg
    45901 gctatagctc agctgggaga gcgcttgcat ggcatgcaag aggtcagcgg ttcgatcccg
    45961 cttagctcca ccaaatttcc aaccctcgct gcaaagcggg ggttttttgt ctctgctttt
    46021 tgccgctttt gtaatacagt ctacgtccgg gttagtgccg cctggtgaaa gcatcattgg
    46081 atgaaaaatc ggcaacagga tggcccctgt ttgcttcgca atgcgaataa acttattatt
    46141 tgtgtgcctg aaaaccccga tcagtgagag tagtgtactc atgtttgtgg agcataacct
    46201 gataaaaaat atcaagatat tcacactagc gtttacgctc accgtggtac ttattcagct
    46261 atcccgtttt atttcgccac ttgccattat ccattccagt tatatctttc tggcgtggat
    46321 gccactgtgc gtaatgctgt caatcttgtt tatctttggc tggcgcggtg tcgttcccgt
    46381 tttatgcggg atgttttgca ccaatctgtg gaactttcat ctctcttttt tacagactgc
    46441 ggtcatgctc ggcagccaga catttgtcgt gttgtgtgcc tgcgcaatat tacgctggca
    46501 gctggggacg cgttggcgtt atggattgac cagccgatat gtctggcaac gtctgttctg
    46561 gcttggtttg gtgacgccga tcggcatcaa atgcagcatg tatcttgtgg ggagcttctt
    46621 tgatttcccg ctaaagatat ccaccttttt cggcgatgcg gatgccattt tcacggtcgt
    46681 ggatttacta agccttttca ccgctgttct gatttacaac atgcttttct actatctcac
    46741 tcgcatgatt gtaagtcccc actttgcgca gatattgtgg cgcagggata tcgctccgtc
    46801 attgggcaaa gagaaacgcg catttacctt aagctggctg gcagcgttaa gcgtgctgct
    46861 acttctgctg tgcacacctt atgaaaacga ctttattgca ggttacctgg tacccgtttt
    46921 cttcatcatc tttaccctcg gggtcggtaa gcttcgctat ccgtttttaa atctcacctg
    46981 ggctgtttca acgctttgcc tgctgaatta caaccagaac tttttgcaag gggtggaaac
    47041 cgaatattcg ctggcattta ttctcgcggt gctgatttcc tttagcgttt gcctgctcta
    47101 tatggtgcgc atttatcatc gcagtgaatg gcttaatcgc cgctggcatt tgcaggcgct
    47161 gaccgatccg ttaacgctcc tgcccaactt tcgtgcgttg gaacaagcgc cggagcaaga
    47221 ggcgggcaag agtttttgct gcctgcgcat tgataatctt gagtttatga gtcgtcatta
    47281 cgggttgatg atgcgcgttc actgtatccg atcaatttac cgtacgctgc tgccattgat
    47341 gcaggaaaac gaaaagttgt atcaattgcc gggtagtgaa ctgctgttag tgctgagcgg
    47401 gccggaaacg gaagggcgac tccagcatat ggttaacatc ctgaatagtc ggcaaattca
    47461 ctggaacaat accgggctgg atatgggcta tggtgctgcc tgggggcgtt ttgatggaaa
    47521 tcaggaaacc ctgcaaccct tgttggggca gttaagctgg ctggcggagc aatcctgcgc
    47581 acatcatcat gtgctggcgc tggatagccg agaggagatg gtttccgggc agaccactaa
    47641 acaggtgcta ttgctgaata ccattcgcac ggcgttagat cagggtgatt tgctgctcta
    47701 cgcccagcca attcgcaaca aagagggtga aggttatgat gagatcctcg cgcgactgaa
    47761 atatgacggc ggcattatga ccccggataa gtttctgccc cttattgctc agtttaatct
    47821 tagcgcgcgt tttgatttgc aagtgctgga atccttgttg aagtggctgg caacacaccc
    47881 ttgcgacaaa aaaggcccgc gcttttcagt taatttaatg ccgctcacgc tgctgcaaaa
    47941 gaatattgcc gggcggatta ttcgtctatt taagcgttat cacatctccc cgcaggcggt
    48001 cattcttgag atcaccgagg agcaggcgtt ttctaacgca gaaagcagca tgtacaacat
    48061 cgagcagctg cataagtttg gtttccggat tgcgattgat gactttggca ccggctatgc
    48121 caactacgaa cggttaaagc gtttgcaggc tgatatcatc aaaattgatg gcgtctttgt
    48181 gaaagatatt gtcacgaata cgctggatgc gatgattgtg agatcaatta ccgatctggc
    48241 gaaagcgaag tcattgagtg tggtcgcgga gtttgtcgag acgcaacagc agcaggcgtt
    48301 attgcataag ctcggggtgc aatatctgca ggggtatttg attggtcgcc cgcagccatt
    48361 agctgattaa caggcgtaaa aaaaccgggg aattatcccc ggctttttta tgtatggtct
    48421 tacagcacca gtgctgcgat tgacgcagac agcacactca ccagggtaga gccgtaaacc
    48481 agtttcagac cgaagcgaga aaccacatta ccttgctctt cattcaggcc tttaactgca
    48541 cctgcaataa ttccgattga agagaagtta gcgaaggaaa ccaggaacac agagatgatg
    48601 ccttcagcac gcggagagag cgtggaagca attttctgca gatccatcat cgcaacgaac
    48661 tcgttagaaa ccagtttggt cgccatgata ctgcccactt gcagtgcttc gctggacgga
    48721 acacccatca cccatgcaat cggatagaag atgtagccca ggatgccctg gaaggagatg
    48781 ctgtagccaa accagccagt cacggtggca aacagtgcgt tcagcgcggc gatcagggcg
    48841 ataaagccga tcagcatcgc agcaacgata atggcaactt tgaaacctgc cagaatgtat
    48901 tcacccagca tttcgaagaa gctctgacct tcgtgcaggt tggacatctg aatgttttct
    48961 tcactggcat caacacggta agggttgatc agcgacagca cgataaaggt gctgaacata
    49021 ttcagtacca gcgctgcaac gacatatttc ggctccagca tggtcatgta tgcaccaacg
    49081 atggacatag aaacggtgga catcgccgtt gctgccatgg tgtacatacg gttacgggag
    49141 attttgccaa ggatatcttt ataggcaata aagttttcag actgacccag aatcagggag
    49201 ctgacggcgt taaaggattc cagtttgccc ataccgttga ctttggagag caggaaacca
    49261 attgcgcgga taatcaccgg caacacgcga atgtgctgga gaataccgat cagtgcagag
    49321 ataaagacga ttgggcacag cactttcagg aagaagaatg ccaggccttg atcattcatg
    49381 ctaccaaaga cgaagttagt cccttcgttg gcaaatccga gcagtttttc gaacatttcg
    49441 gagaagcctt tcacgaagcc taaaccaacg tcggagttca ggaagaacca cgccagtaac
    49501 acttcgataa caagcagttg aataacataa cggatacgaa tttttttgcg gtcgctgctt
    49561 accagcagtg cgagaatcgc aacaacggca agtgccagta caaaatgaag gacgcggtcc
    49621 atatttgctc caaatatgag gcaggttaaa tttccgtgca cattctatgt aacatggtta
    49681 aggaaaacga gatctgacac acactataat gacgtcctgt gacaagcttc aaaaatagtg
    49741 agctgtcata catttctgct atgttgtgta tggaagctga aagttatgta aatgtgctaa
    49801 cattaaacat aaccataaca ttcaagtcat ca
//
LOCUS       DAZKFU020000005        48920 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZKFU020000005 DAZKFU020000000
VERSION     DAZKFU020000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 48920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.005"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            370..1587
                     /gene="alaA"
                     /locus_tag="ACHOQQ_RS01100"
                     /old_locus_tag="pgaptmp_000220"
     CDS             370..1587
                     /gene="alaA"
                     /locus_tag="ACHOQQ_RS01100"
                     /old_locus_tag="pgaptmp_000220"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGU4468528.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1671..2270
                     /gene="yfbR"
                     /locus_tag="ACHOQQ_RS01105"
                     /old_locus_tag="pgaptmp_000221"
     CDS             1671..2270
                     /gene="yfbR"
                     /locus_tag="ACHOQQ_RS01105"
                     /old_locus_tag="pgaptmp_000221"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4468529.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2329..4161)
                     /gene="yfbS"
                     /locus_tag="ACHOQQ_RS01110"
                     /old_locus_tag="pgaptmp_000222"
     CDS             complement(2329..4161)
                     /gene="yfbS"
                     /locus_tag="ACHOQQ_RS01110"
                     /old_locus_tag="pgaptmp_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU4468530.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4248..4898)
                     /gene="hxpA"
                     /locus_tag="ACHOQQ_RS01115"
                     /old_locus_tag="pgaptmp_000223"
     CDS             complement(4248..4898)
                     /gene="hxpA"
                     /locus_tag="ACHOQQ_RS01115"
                     /old_locus_tag="pgaptmp_000223"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGU4468531.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLDQITVTKQPNGDVIIQ"
     gene            complement(4909..5403)
                     /gene="yfbU"
                     /locus_tag="ACHOQQ_RS01120"
                     /old_locus_tag="pgaptmp_000224"
     CDS             complement(4909..5403)
                     /gene="yfbU"
                     /locus_tag="ACHOQQ_RS01120"
                     /old_locus_tag="pgaptmp_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGU4468532.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5486..5941)
                     /gene="yfbV"
                     /locus_tag="ACHOQQ_RS01125"
                     /old_locus_tag="pgaptmp_000225"
     CDS             complement(5486..5941)
                     /gene="yfbV"
                     /locus_tag="ACHOQQ_RS01125"
                     /old_locus_tag="pgaptmp_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGU4468533.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6279..7481
                     /gene="ackA"
                     /locus_tag="ACHOQQ_RS01130"
                     /old_locus_tag="pgaptmp_000226"
     CDS             6279..7481
                     /gene="ackA"
                     /locus_tag="ACHOQQ_RS01130"
                     /old_locus_tag="pgaptmp_000226"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGU4468534.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKDSGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7556..9700
                     /gene="pta"
                     /locus_tag="ACHOQQ_RS01135"
                     /old_locus_tag="pgaptmp_000227"
     CDS             7556..9700
                     /gene="pta"
                     /locus_tag="ACHOQQ_RS01135"
                     /old_locus_tag="pgaptmp_000227"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4468535.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9890..11410
                     /gene="yfcC"
                     /locus_tag="ACHOQQ_RS01140"
                     /old_locus_tag="pgaptmp_000228"
     CDS             9890..11410
                     /gene="yfcC"
                     /locus_tag="ACHOQQ_RS01140"
                     /old_locus_tag="pgaptmp_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGU4468536.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVIFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11443..11985)
                     /gene="yfcD"
                     /locus_tag="ACHOQQ_RS01145"
                     /old_locus_tag="pgaptmp_000229"
     CDS             complement(11443..11985)
                     /gene="yfcD"
                     /locus_tag="ACHOQQ_RS01145"
                     /old_locus_tag="pgaptmp_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGU4468537.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12043..12594)
                     /gene="yfcE"
                     /locus_tag="ACHOQQ_RS01150"
                     /old_locus_tag="pgaptmp_000230"
     CDS             complement(12043..12594)
                     /gene="yfcE"
                     /locus_tag="ACHOQQ_RS01150"
                     /old_locus_tag="pgaptmp_000230"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGU4468538.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12650..13294)
                     /gene="yfcF"
                     /locus_tag="ACHOQQ_RS01155"
                     /old_locus_tag="pgaptmp_000231"
     CDS             complement(12650..13294)
                     /gene="yfcF"
                     /locus_tag="ACHOQQ_RS01155"
                     /old_locus_tag="pgaptmp_000231"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGU4468539.1"
                     /translation="MSKPAITLWSDAHCFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13430..14077
                     /gene="yfcG"
                     /locus_tag="ACHOQQ_RS01160"
                     /old_locus_tag="pgaptmp_000232"
     CDS             13430..14077
                     /gene="yfcG"
                     /locus_tag="ACHOQQ_RS01160"
                     /old_locus_tag="pgaptmp_000232"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGU4468540.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14134..14496
                     /gene="folX"
                     /locus_tag="ACHOQQ_RS01165"
                     /old_locus_tag="pgaptmp_000233"
     CDS             14134..14496
                     /gene="folX"
                     /locus_tag="ACHOQQ_RS01165"
                     /old_locus_tag="pgaptmp_000233"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGU4468541.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14517..15410
                     /gene="yfcH"
                     /locus_tag="ACHOQQ_RS01170"
                     /old_locus_tag="pgaptmp_000234"
     CDS             14517..15410
                     /gene="yfcH"
                     /locus_tag="ACHOQQ_RS01170"
                     /old_locus_tag="pgaptmp_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGU4468542.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCTRWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15458..16348)
                     /gene="rpnB"
                     /locus_tag="ACHOQQ_RS01175"
                     /old_locus_tag="pgaptmp_000235"
     CDS             complement(15458..16348)
                     /gene="rpnB"
                     /locus_tag="ACHOQQ_RS01175"
                     /old_locus_tag="pgaptmp_000235"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGU4468543.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLTAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16545..17318)
                     /gene="hisP"
                     /locus_tag="ACHOQQ_RS01180"
                     /old_locus_tag="pgaptmp_000236"
     CDS             complement(16545..17318)
                     /gene="hisP"
                     /locus_tag="ACHOQQ_RS01180"
                     /old_locus_tag="pgaptmp_000236"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGU4468544.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGAIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17326..18042)
                     /gene="hisM"
                     /locus_tag="ACHOQQ_RS01185"
                     /old_locus_tag="pgaptmp_000237"
     CDS             complement(17326..18042)
                     /gene="hisM"
                     /locus_tag="ACHOQQ_RS01185"
                     /old_locus_tag="pgaptmp_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4468545.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18039..18725)
                     /gene="hisQ"
                     /locus_tag="ACHOQQ_RS01190"
                     /old_locus_tag="pgaptmp_000238"
     CDS             complement(18039..18725)
                     /gene="hisQ"
                     /locus_tag="ACHOQQ_RS01190"
                     /old_locus_tag="pgaptmp_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGU4468546.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18815..19597)
                     /gene="hisJ"
                     /locus_tag="ACHOQQ_RS01195"
                     /old_locus_tag="pgaptmp_000239"
     CDS             complement(18815..19597)
                     /gene="hisJ"
                     /locus_tag="ACHOQQ_RS01195"
                     /old_locus_tag="pgaptmp_000239"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGU4468547.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQKIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19818..20600)
                     /gene="argT"
                     /locus_tag="ACHOQQ_RS01200"
                     /old_locus_tag="pgaptmp_000240"
     CDS             complement(19818..20600)
                     /gene="argT"
                     /locus_tag="ACHOQQ_RS01200"
                     /old_locus_tag="pgaptmp_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGU4468548.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20866..21435)
                     /gene="ubiX"
                     /locus_tag="ACHOQQ_RS01205"
                     /old_locus_tag="pgaptmp_000241"
     CDS             complement(20866..21435)
                     /gene="ubiX"
                     /locus_tag="ACHOQQ_RS01205"
                     /old_locus_tag="pgaptmp_000241"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGU4468549.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21530..23047)
                     /gene="purF"
                     /locus_tag="ACHOQQ_RS01210"
                     /old_locus_tag="pgaptmp_000242"
     CDS             complement(21530..23047)
                     /gene="purF"
                     /locus_tag="ACHOQQ_RS01210"
                     /old_locus_tag="pgaptmp_000242"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGU4468550.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23084..23572)
                     /gene="cvpA"
                     /locus_tag="ACHOQQ_RS01215"
                     /old_locus_tag="pgaptmp_000243"
     CDS             complement(23084..23572)
                     /gene="cvpA"
                     /locus_tag="ACHOQQ_RS01215"
                     /old_locus_tag="pgaptmp_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGU4468551.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(24013..24675)
                     /gene="dedD"
                     /locus_tag="ACHOQQ_RS01220"
                     /old_locus_tag="pgaptmp_000244"
     CDS             complement(24013..24675)
                     /gene="dedD"
                     /locus_tag="ACHOQQ_RS01220"
                     /old_locus_tag="pgaptmp_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGU4468552.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24665..25933)
                     /gene="folC"
                     /locus_tag="ACHOQQ_RS01225"
                     /old_locus_tag="pgaptmp_000245"
     CDS             complement(24665..25933)
                     /gene="folC"
                     /locus_tag="ACHOQQ_RS01225"
                     /old_locus_tag="pgaptmp_000245"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGU4468553.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(26003..26917)
                     /gene="accD"
                     /locus_tag="ACHOQQ_RS01230"
                     /old_locus_tag="pgaptmp_000246"
     CDS             complement(26003..26917)
                     /gene="accD"
                     /locus_tag="ACHOQQ_RS01230"
                     /old_locus_tag="pgaptmp_000246"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGU4468554.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(27073..27732)
                     /gene="dedA"
                     /locus_tag="ACHOQQ_RS01235"
                     /old_locus_tag="pgaptmp_000247"
     CDS             complement(27073..27732)
                     /gene="dedA"
                     /locus_tag="ACHOQQ_RS01235"
                     /old_locus_tag="pgaptmp_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4468555.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27815..28627)
                     /gene="truA"
                     /locus_tag="ACHOQQ_RS01240"
                     /old_locus_tag="pgaptmp_000248"
     CDS             complement(27815..28627)
                     /gene="truA"
                     /locus_tag="ACHOQQ_RS01240"
                     /old_locus_tag="pgaptmp_000248"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGU4468556.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRFDLPKPPMGPLFLAD"
     gene            complement(28627..29640)
                     /gene="usg"
                     /locus_tag="ACHOQQ_RS01245"
                     /old_locus_tag="pgaptmp_000249"
     CDS             complement(28627..29640)
                     /gene="usg"
                     /locus_tag="ACHOQQ_RS01245"
                     /old_locus_tag="pgaptmp_000249"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4468557.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29706..30842)
                     /gene="pdxB"
                     /locus_tag="ACHOQQ_RS01250"
                     /old_locus_tag="pgaptmp_000250"
     CDS             complement(29706..30842)
                     /gene="pdxB"
                     /locus_tag="ACHOQQ_RS01250"
                     /old_locus_tag="pgaptmp_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGU4468558.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKKAGIGFSAAPGCNAIAVVEYVLSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRNLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHIALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30941..31936
                     /gene="flk"
                     /locus_tag="ACHOQQ_RS01255"
                     /old_locus_tag="pgaptmp_000251"
     CDS             30941..31936
                     /gene="flk"
                     /locus_tag="ACHOQQ_RS01255"
                     /old_locus_tag="pgaptmp_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGU4468559.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31933..33111)
                     /gene="yfcJ"
                     /locus_tag="ACHOQQ_RS01260"
                     /old_locus_tag="pgaptmp_000252"
     CDS             complement(31933..33111)
                     /gene="yfcJ"
                     /locus_tag="ACHOQQ_RS01260"
                     /old_locus_tag="pgaptmp_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HGU4468560.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33420..34640)
                     /gene="fabB"
                     /locus_tag="ACHOQQ_RS01265"
                     /old_locus_tag="pgaptmp_000253"
     CDS             complement(33420..34640)
                     /gene="fabB"
                     /locus_tag="ACHOQQ_RS01265"
                     /old_locus_tag="pgaptmp_000253"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGU4468561.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34799..36805
                     /gene="mnmC"
                     /locus_tag="ACHOQQ_RS01270"
                     /old_locus_tag="pgaptmp_000254"
     CDS             34799..36805
                     /gene="mnmC"
                     /locus_tag="ACHOQQ_RS01270"
                     /old_locus_tag="pgaptmp_000254"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGU4468562.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHLHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEVPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLKLAQQQGLQIYYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDISEKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLVEQKDKAVSAPVYDDLFMLDALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36926..37204)
                     /gene="yfcL"
                     /locus_tag="ACHOQQ_RS01275"
                     /old_locus_tag="pgaptmp_000255"
     CDS             complement(36926..37204)
                     /gene="yfcL"
                     /locus_tag="ACHOQQ_RS01275"
                     /old_locus_tag="pgaptmp_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGU4468563.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37238..37786)
                     /gene="epmC"
                     /locus_tag="ACHOQQ_RS01280"
                     /old_locus_tag="pgaptmp_000256"
     CDS             complement(37238..37786)
                     /gene="epmC"
                     /locus_tag="ACHOQQ_RS01280"
                     /old_locus_tag="pgaptmp_000256"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGU4468564.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37786..38595)
                     /gene="yfcA"
                     /locus_tag="ACHOQQ_RS01285"
                     /old_locus_tag="pgaptmp_000257"
     CDS             complement(37786..38595)
                     /gene="yfcA"
                     /locus_tag="ACHOQQ_RS01285"
                     /old_locus_tag="pgaptmp_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU4468565.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38595..39419)
                     /gene="mepA"
                     /locus_tag="ACHOQQ_RS01290"
                     /old_locus_tag="pgaptmp_000258"
     CDS             complement(38595..39419)
                     /gene="mepA"
                     /locus_tag="ACHOQQ_RS01290"
                     /old_locus_tag="pgaptmp_000258"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGU4468566.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKRVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39423..40508)
                     /gene="aroC"
                     /locus_tag="ACHOQQ_RS01295"
                     /old_locus_tag="pgaptmp_000259"
     CDS             complement(39423..40508)
                     /gene="aroC"
                     /locus_tag="ACHOQQ_RS01295"
                     /old_locus_tag="pgaptmp_000259"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGU4468567.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40543..41475)
                     /gene="prmB"
                     /locus_tag="ACHOQQ_RS01300"
                     /old_locus_tag="pgaptmp_000260"
     CDS             complement(40543..41475)
                     /gene="prmB"
                     /locus_tag="ACHOQQ_RS01300"
                     /old_locus_tag="pgaptmp_000260"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4468568.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            41641..42192
                     /gene="smrB"
                     /locus_tag="ACHOQQ_RS01305"
                     /old_locus_tag="pgaptmp_000261"
     CDS             41641..42192
                     /gene="smrB"
                     /locus_tag="ACHOQQ_RS01305"
                     /old_locus_tag="pgaptmp_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGU4468569.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42306..43145)
                     /locus_tag="ACHOQQ_RS01310"
                     /old_locus_tag="pgaptmp_000262"
     CDS             complement(42306..43145)
                     /locus_tag="ACHOQQ_RS01310"
                     /old_locus_tag="pgaptmp_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGU4468570.1"
                     /translation="MKIKVILFGMLLFGLNSSVQADVTFNNVQTYGASYGSGLTTSIV
                     YKIDVLTPEGAYYGTYSQGGIMVQAGVVPNLTWTGPGVPPDITIPPGRAPIVDDSYCP
                     GMTSGWVCHRLPVNVHVTGTTHGCPWVVTTSIYSTARPPQAGPYYYQGPNAHQSNCPT
                     EPVASYDISWDENTVVHNKSLSLQSTGGVIEKSLPTFLMKDGRLCDGSDFSDDRGAYC
                     RLVSQLMTFSVQGCDNNKVNVTANRHPVTDKQLHDMQVRVDTSERQSFDATCRFQYIL
                     NEL"
     gene            complement(43146..43664)
                     /locus_tag="ACHOQQ_RS01315"
                     /old_locus_tag="pgaptmp_000263"
     CDS             complement(43146..43664)
                     /locus_tag="ACHOQQ_RS01315"
                     /old_locus_tag="pgaptmp_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4468571.1"
                     /translation="MKTWFALIVMLLLAGNSTARADNNLHFYGNLLSKSCTLVVQGQV
                     LAEVHFPSISSRDLMSREQSDRVPVVFQLKDCKGPVGYKVRVTLTGTEDSDLPGFLAL
                     DASSGAQGVSIGMETVDGIQVPINNSAGAIFNLSNGANNLNFKAWLQAKSGREVTSGE
                     FTASLTAIFEYI"
     gene            complement(43667..44143)
                     /locus_tag="ACHOQQ_RS01320"
                     /old_locus_tag="pgaptmp_000264"
     CDS             complement(43667..44143)
                     /locus_tag="ACHOQQ_RS01320"
                     /old_locus_tag="pgaptmp_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4468572.1"
                     /translation="MKILLYLLLCMSSGIVNASPDITFKGTLVLPPACTISDGNTIEV
                     EFRDVIIDSIDGNNGREVVPYDIKCDAVTPGSSWDMTLTWIGTQTSYDDAAIETDVTG
                     LGIKLRQNGQPFRVNTPIQINADTKPQLEAVPVKAVDAVLTDGTFSASATLRVEYQ"
     gene            complement(44140..44643)
                     /locus_tag="ACHOQQ_RS01325"
                     /old_locus_tag="pgaptmp_000265"
     CDS             complement(44140..44643)
                     /locus_tag="ACHOQQ_RS01325"
                     /old_locus_tag="pgaptmp_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4468573.1"
                     /translation="MRQIFVVGVGILLLGVSGIYNIILANSKPVDLILQVHVDPPPPC
                     SVTGSSVDFQTVNINDINGSNYRKDAGYTLSCPDRKADDLRMQLRGNTTTINGEKVVD
                     VGVPGFGIRIENADNNSLFSIGENNWTPFNITSQPKLNAVPVKQSGAQLSGADFSGSL
                     TMVVDYQ"
     gene            complement(44659..45411)
                     /locus_tag="ACHOQQ_RS01330"
                     /old_locus_tag="pgaptmp_000266"
     CDS             complement(44659..45411)
                     /locus_tag="ACHOQQ_RS01330"
                     /old_locus_tag="pgaptmp_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4468574.1"
                     /translation="MTNLFCSAKKSTMLLALLLTTASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDEQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTRSGQEFTVNNPTPYYVVISNASTQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LSYVNDYGARMPLYFKCGGDTCQVDVEQSRKG"
     gene            complement(45434..48073)
                     /locus_tag="ACHOQQ_RS01335"
                     /old_locus_tag="pgaptmp_000267"
     CDS             complement(45434..48073)
                     /locus_tag="ACHOQQ_RS01335"
                     /old_locus_tag="pgaptmp_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU4468575.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAEDEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDVTHSHTRLDKETAYGK
                     GSLDGNSFRVSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNIMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQSDKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPMIQEQTQQVMPTEAPVSVSAN"
     gene            complement(48155..48718)
                     /gene="yfcV"
                     /locus_tag="ACHOQQ_RS01340"
                     /old_locus_tag="pgaptmp_000268"
     CDS             complement(48155..48718)
                     /gene="yfcV"
                     /locus_tag="ACHOQQ_RS01340"
                     /old_locus_tag="pgaptmp_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4468576.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12024 a        13160 c        12127 g        11609 t
ORIGIN      
        1 gtttcacacc ttgcccccat tgcgacacct cggaaatcat cacgcggtta aactctccgt
       61 aatgcggaaa tatcatcatc gcgtttcatt agtgaattct taaatgaggc attttcacac
      121 aattatctta cagataaaaa aaccagactt acaattaaga atcagaacaa ttcactatat
      181 aacattgcat gtaaagcata tacacctcat tattttgtca ttattaagtt attaacagca
      241 caatcgagcc ttcccctctg gcaaaatctt attctgcaga ccttcaaaac accgtcctgg
      301 gggagtacat tgttctaagc tgacttccac ggcagggagt ggcgataaca gcaaaaaagg
      361 tcaagattca tgtcccccat tgaaaaatcc agcaaattag agaatgtctg ttatgacatc
      421 cgtggtccgg tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa
      481 ctgaacatcg gcaacccagc cccgttcggt tttgacgcgc cagatgaaat cctcgttgac
      541 gtgatacgca acctgcctac cgctcaaggg tattgcgatt ccaaaggtct ttactccgcg
      601 cgtaaagcca tcatgcagca ctatcaggct cgtggcatgc gtgatgttac cgtggaagat
      661 atttacatcg gcaatggtgt atcggagctt atcgttcagg caatgcaggc attgctgaac
      721 agcggggacg aaatgttggt tcctgcacca gattacccac tatggaccgc ggcggtttcg
      781 ctttccagcg gtaaagcggt gcattatctt tgcgatgaat cctctgactg gttcccggac
      841 ctcgatgata ttcgcgctaa aattacgcct cgtacgcgtg ggatcgttat tatcaaccca
      901 aataacccaa ccggcgcggt atattccaaa gagcttttaa tggagattgt ggagattgca
      961 cgtcagcata atctcattat cttcgccgat gaaatttatg acaaaattct ctacgacgac
     1021 gctgagcatc actcaattgc gccgctggca cctgacctgc tgaccattac ctttaacgga
     1081 ctgtcgaaaa cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg
     1141 aaaaaacacg ccaaaggcta catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt
     1201 gctaacgttc ctgcgcaaca cgccattcag accgcgctgg gtggttatca gagcatcagt
     1261 gaatttatta cccctggcgg tcgtctttat gagcagcgta accgcgcgtg ggaattgatc
     1321 aacgatattc cgggcgtttc ctgcgtgaaa cctcgtggtg cgctgtacat gttcccgaaa
     1381 atcgacgcca aacgctttaa cattcacgac gatcagaaaa tggtgctgga tttcctgttg
     1441 caggaaaaag ttctgttggt gcaagggacg gcattcaact ggccgtggcc ggatcacttc
     1501 cgcattgtca cgctaccgcg tgtcgatgat atcgagctgt ctttgagcaa gttcgcgcgt
     1561 ttcctttctg gttatcatca gctgtaatct taatttcact gccggagatt gcatccggca
     1621 gtgttatccc gccacaatga cctgatgatg tcatcatacg taaggtcact atgaaacaga
     1681 gccatttttt tgcccatctc tcccgcctga aactcattaa ccgctggccg ctcatgcgca
     1741 acgtgcggac ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc
     1801 tggcagctat caaaaatcgg aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac
     1861 tggcgatgta ccacgatgcc tcagaagtgc tcaccggcga tctccctact ccggtgaaat
     1921 acttcaattc gcaaatcgct caggagtaca aggctattga aaaaatcgct cagcaaaaac
     1981 tggtcgatat ggttccggaa gagctgcggg atatctttgc gccgttaatt gacgagcatg
     2041 catatagcga tgaagaaaaa tcgctggtga aacaggcaga tgcactgtgt gcatatctga
     2101 aatgtctgga agaactcgcg gccggaaata atgaattctt gctggcaaaa acgcgactgg
     2161 aagcgacgct tgaagcgcgt cgcagccagg agatggacta cttcatggaa gtatttgttc
     2221 ccagcttcca tctttcgctc gatgagatta gccaggattc accgctgtaa gcagcgggag
     2281 tctgcgtcgc atcaggcaat aagcgccgga tgcgacatca ggctcttgtc aaaacggaaa
     2341 cagcatcggg atcatcacca cacaaaccgc catcacgata atggtgaacg gtacccccag
     2401 cttcacaaag tcactgaagc tgtaatttcc cggacctaaa accagtgtgt taaccggtga
     2461 agaaaccggt gtcataaagg cggcggatgc tgccatcgcc acgaccatcg cgaatggata
     2521 aggcgacacc cccatcgttt tggcagcagc cagcgcaatc ggagccatca acaccgccgt
     2581 cgcggtatta gagataaata gcccaataac cgccgacaag acaaacaaac aacccagcat
     2641 catatgtggc ccgtaaccac cgccaatgtc catcagccct ttcaccgcca gcgcgacacc
     2701 tcccgttttc tgtaatgcca cagcaaacgg catcatccca acgatcaaaa taatgctcgg
     2761 ccagtgaatg gatttatagg cgctttcagc atctatacag cggaatttcc ccatcagcag
     2821 gcaggcgatg atagcggcaa caggattagg aatttcatct gtcagcatta acgccaccat
     2881 tagcaccaga cagaaaatgg catggggtgc ctggctgtgc gcgggcgatg cttcactcac
     2941 ctcttccggt aagttcagcg ctacgaagtc gcggccctgt ttggccagca taccgatcag
     3001 tttccagtta cccacaacca ggatgatatc gcccagcagc agaggctcat ccgccagcga
     3061 accttccagc gccacgccat tgcgctttag ccccaccaca ttcagtccgt agcgggtacg
     3121 aaaaccaatt tcgcgcaccg atttaccaat cagttctgac tcaggaatta atgaaatctc
     3181 tgccatgccc acatcaaggg cctggtcaga aaaatactcg ccgcgcagta ccatcggctc
     3241 cagcaattgc tcactacaaa attgccggag atcgacatca gccgcagaca tatcaataag
     3301 caaaacgtca cgcgcgcgaa attcagaaac cccattaacg ttcacgataa cgcgacgaaa
     3361 acgccgccag cgttcaacac cgatgacgtt agcgccataa cgctcacgta atttgagatc
     3421 atccagccgt tgaccaatca ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc
     3481 ggtcagtcga tattcacgga taagatcgcg aaaggttcga cgcgtccagc cttcgcgctg
     3541 cggggtctgg gtatcccctt tcagcatgaa acgcatcact aacatataca aaatacccag
     3601 caccagcacg accaggccaa taggtgttac gctaaagaaa ctgaagccgt gatagccttc
     3661 acgcagcaat tcactgttga ctaccaggtt cggcggcgtc gccaccagcg tcatcatgcc
     3721 gctaatcagc ccggcaaaac ttaacggcat catcagacgt gacggcgaag tttgcatacg
     3781 catggcaacg cttaacacca cgggaataaa gatagcgaca acgccggttg aactcataaa
     3841 cgcgccaagc cccgcgacgg tcagcatcaa caaaaccaac attttgattt cactgttgcc
     3901 cgcaactttg accagccatg ttcccattac ggtggcaaca ccggtacgga ccaaaccatc
     3961 gccaataata aacaaggcgg caatcaggac aacgttagga tcagaaaagc cggaaaatac
     4021 ttctgggact gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc
     4081 atccatacgc actctgcccg tcgcaaacaa gacgatggca acggccagta atgaaagaac
     4141 ccaaatcaat tcaccgttca caacttatcc ttgttaattg agggggatga cttgattctg
     4201 ccataaaaaa gccccgacga gacggggcta aatcatgatc aggtgtttca ctgaataata
     4261 acatcgccat ttggctgttt ggtcacagta atttgatcca gactgtggag gaccaaatcg
     4321 acctcattca ggcgcggggt atctgccgga gcgttaaccg caatgacatg gcaacccgcc
     4381 gccaggccag aaagcacgcc agcgggagca tcttccacca ccacacactc ctgcggcgca
     4441 agccccagca gctgtgcgcc taacagatac gcatcaggtt ctggttttcc gcgcttcact
     4501 cgctcagcgg tcacaaacac ctctggcgcg ggaagccccg ctattttatg gcgcgctcgc
     4561 gctaccggca tggagccaga agtcacaatg gcccacggaa tacctgcttt attcaaatga
     4621 ttgagtaagg caattgcccc cggaagcgcg gtaatacctt cggtttccgt ggcctcgatt
     4681 tgctccagac gcgtaaactc ggcggcaata tcagcctcgg atttgcccgc cataaaatgg
     4741 cgcagagagg tgatcgcctg tttaccgtga atgaaagcca gcacctcttc cggcgctaac
     4801 ccatgacgtc tggcccagtt gctccacgcc cgttctaccg caggcaggga atccaccagc
     4861 gttccatcaa gatcaaacag aaaacctttg caccgcacgc gggcctcctc aggcgttgat
     4921 aatttgatta atttcgttgg cgctcaaatg gtactgacgc gggcaggcat gccacacatt
     4981 aagcatgcgc tgatattttt cccacattgg cgtctgggcg ttaaaaccgt gagttccggc
     5041 gtcaaaatgg gtatagcgcc cttccacatt aaccataaag cggacataac cgaggtaacg
     5101 tgcttcagtg gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga tggattgctg
     5161 gtcctgcaaa ttagaccagg aaacatgcaa cgcatgatac atctccataa tgtcgatgat
     5221 agtgcggcag gtttcttctt tcagctcgcc aaactcgcga tccagttcac gcatctgtaa
     5281 tccataacca cgctcaatga ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc
     5341 gagcatagtc atcatcttgt actggttaga caaaataaga cgttgcgcgt tggtcatttc
     5401 cattgttgac tcctgtatca ctctattacg gtgaaaaaaa agaaggctga gtatgccttc
     5461 ttttatatgc gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt
     5521 tgaaggcgcg cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg
     5581 ccaacacctg tccagactcc tgcaatttac cgcgaacttc ataaaaccag ttgaggattg
     5641 cagggggtaa tggcgtgaca gaacgcttgc ccagccacca caatccctgc atgggtaaac
     5701 ttaaggcgaa cagggcagtg gcaactgccg gcccaagctg accgcccagg gcaatctgcc
     5761 agcagagagt aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga
     5821 taacgcgatt ttcgacaaag accggggcaa ggcgtttttc cagcggccac gtctttgagt
     5881 aatgctgtcc ccggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca
     5941 tgcttcacct caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg
     6001 ggacaacgtt caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg
     6061 aaggcctaag tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct
     6121 gaaaattacg caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg
     6181 cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct
     6241 gacgtttttt tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg
     6301 ttctgaactg cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag
     6361 agtacctttc tggtttagcc gaatgtttcc acctgcccga agcacgtatc aaatggaaaa
     6421 tggacggcaa taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca
     6481 actttatcgt taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta
     6541 tcggtcaccg tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt
     6601 ctgttattca gggtatcaaa gatgcagctt cttttgcacc gctgcacaac ccggctcacc
     6661 tgatcggtat cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg
     6721 tatttgacac cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgccgt
     6781 acaacctgta caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct
     6841 atgtaaccca ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca
     6901 cctgccacct gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca
     6961 cctctatggg cctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg
     7021 atccggcgat catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca
     7081 aactgctgac caaagattct ggcctgctgg gtctgaccga agtgaccagc gactgccgct
     7141 atgttgaaga caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc
     7201 accgcctggc gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg
     7261 ttgtattcac cggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca
     7321 aactgggcgt gctgggcttt gaagttgatc atgaacgcaa cctggctgca cgtttcggca
     7381 aatctggttt catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag
     7441 aactggttat cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag
     7501 ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc
     7561 ccgtattatt atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg
     7621 cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca
     7681 gccgcgtacc ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc
     7741 caccacgacg gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa
     7801 tcagaaagat gtgctgatgg aagagatcgt cgcaaactac cacgctaaca ccaaagacgc
     7861 tgaagtcgtt ctggttgaag gtctggtccc gacacgtaag caccagtttg cccagtctct
     7921 gaactacgaa atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac
     7981 tgacaccccg gaacagctga aagagcgtat cgaactgacc cgcaacagct tcggcggtgc
     8041 caaaaacacc aacatcaccg gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca
     8101 gggtcgtact cgcccggatc tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa
     8161 caatgttgat ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc
     8221 gtggagcttt gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac
     8281 catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg
     8341 cagcattccg cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga
     8401 ccgtcctgac gtgctggttg ccgcttgcct ggctgccatg aacggcgtag aaatcggtgc
     8461 cctgctgctg actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc
     8521 tttcgctacc ggcctgccgg tatttatggt gaacaccaac acctggcaga cttctctgag
     8581 cctgcagagc ttcaacctgg aagttccagt tgacgatcac gagcgtatcg agaaagttca
     8641 ggaatacgtt gctaactaca tcaacgctga ctggatcgat tctctgactg ccacttctga
     8701 gcgcagccgt cgtctgtctc cgccagcgtt ccgttatcag ctgactgaac ttgcgcgcaa
     8761 agcgggcaaa cgtatcgtac tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc
     8821 tatctgtgct gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa
     8881 ccgtgttgca gcgtctcagg gtgtagaact gggtgcaggc attgaaatcg ttgatccaga
     8941 agtggttcgc gaaagctatg ttggtcgtct ggtcgagctg cgtaagaaca aaggcatgac
     9001 cgaaaccgtt gcccgcgaac agctggaaga caacgtggtg ctcggtacgc tgatgctgga
     9061 acaggatgaa gttgatggtc tggtttccgg tgctgttcac actaccgcaa acaccatccg
     9121 tccgccgctg cagctgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt
     9181 catgctgctg ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac
     9241 cgcagaacag ctggcagaaa tcgcgattca gtccgctgat tccgctgcag ccttcggtat
     9301 cgaaccgcgc gttgctatgc tctcctactc caccggtact tctggtgcag gtagcgacgt
     9361 agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga
     9421 cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcta aagcgccgaa
     9481 ctctccggtt gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca ccggtaacac
     9541 cacctacaaa gcggtacagc gttctgccga cctgatctcc atcgggccga tgctgcaggg
     9601 tatgcgcaag ccggttaacg acctgtcccg tggcgcactg gttgacgata tcgtctacac
     9661 catcgcgctg actgcgattc agtctgcaca gcagcagtaa tctcgtcatc atccgcagct
     9721 ttgcgctgcg gatacctgaa ccggaaatag tcactatttc cggtttttta ttctcttaat
     9781 ttgcattaat cctttctgat tatcttgctt aactgcgctg catcaatgaa ttgcgccatt
     9841 tcactttgca tacttaccac tttgttttgt gcaagggaat atttgcgcta tgtccgcaat
     9901 cactgaatcc aaaccaacaa gaagatgggc aatgcccgat acgttggtga ttatcttttt
     9961 tgttgctatt ttaaccagcc ttgccacctg ggtagttccg gtggggatgt ttgacagtca
    10021 ggaagtgcag tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag atccacactc
    10081 atttcgcatt ctgactaacg aagcaggcga acctgagtat caccgcgtac agctgttcac
    10141 gacgggcgat gaacgcccgg gcctgatgaa cttcccgttt gaagggttaa cctcaggatc
    10201 gaaatacggg acagccgttg gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg
    10261 cattgtgatg cgtacaggaa ccattgataa cggtatcctg gcgcttattc gccatacccg
    10321 cgggaatgaa attctcttta ttcctgcgct gtttattctg ttttcacttg gcggcgcggt
    10381 atttggtatg ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc taatggtccg
    10441 gctgggctat gacagtatta ccaccgtcct ggtgacctat attgccacgc aaatcggttt
    10501 tgccagttcg tggatgaacc cgttttgtgt ggtcgttgct caggggattg ccggcgttcc
    10561 ggtgctttct ggctccgggt tgcgcatcgt ggtgtgggtt atcgccactc tgattggcct
    10621 gatctttacc atggtgtacg cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca
    10681 tgagtccgac cgcttctttc gtgaaaagca ggcggatgtt gaacaacgtc cgtttacctt
    10741 tggtgactgg ctggtattga ttgtcctgac cgccgtaatg gtctgggtga tttggggcgt
    10801 gatcgttaat gcctggttta ttccagaaat tgccagccag ttcttcacca tgggtctggt
    10861 gattggcatc atcggcgtta ttttccgcct taacggcatg acggttaata ccatggcttc
    10921 atcctttacc gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa
    10981 agggattttg ctgctggttg gtaatggtga agcaggtgat gccagcgtgt taaatactat
    11041 cctcaacagc attgccaatg ccattagcgg tctggacaac gcggtcgcgg cctggtttat
    11101 gttgctcttc caggcggtat ttaatttctt cgtgacgtcc ggttctggtc aggcggcgtt
    11161 aaccatgccg ttactggcac cgcttggcga tctggtcggt gttaaccgtc aggttaccgt
    11221 gctggcgttc cagtttggtg atggcttcag tcacatcatt tacccgacct cagcttcgtt
    11281 aatggcgacg ctcggtgttt gccgggtgga cttccgtaac tggctgaagg tgggcgcgac
    11341 cctgcttgga ctgctgttta ttatgtccag cgtcgtagtg atcggcgctc agttgatggg
    11401 ctaccactaa aaatgttaag agccgcaatt gcggctcttt tttcattctg ccgtttcagt
    11461 ctctacggct tcatttttgg cattgcgctt catccacaac gctagcgctt tcagcgagtc
    11521 tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc aaacttcact
    11581 gacttcatct tcctgtaggg cgaagggacc gtgagagacg cagctgaaca atgcgcccca
    11641 gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc
    11701 aatgcccaac tcttcttccg cttcgcgacg cgcggattcc agcagttgct catcggcctg
    11761 aaccacaccg cctgcggtcg catctaacat gccgggtaaa aagtcttttg tctcggtacg
    11821 acgctggacc agaattttgc ccatgccatc atgcacgacg atgtaagttg cacgatgacg
    11881 cagacactgt gcccgcattt gctcccggct ggcttgtgca atgacttcgt tctcttcatt
    11941 gacaatatcc acccattcag tacttgccaa acgacgctgt tccattatca ggaaaccttc
    12001 tttttctggc gctcttacgg cgcattttga gttgtgggta aattacggat taatcgcgac
    12061 ctgcgcaatg atactttgat cattgagtgc gattacacta agtacgtcat tatccagcat
    12121 gccataactc gccggattac cgcctttcgg aatactcacc gagccggggt tgaagtggaa
    12181 aatctccccc cgctgttctg ctactggcag atgggtatga ccgtaaacca gcacatcgtt
    12241 ctggtttaaa gcaggcagat tttccgggcc aaaaagatga ccatgcgtca aaaacagacg
    12301 ttgtttttcc agtaatacct gttgccacgg cgcggttatc gggaaatgca gcagcatttg
    12361 atccacttcg ctgtcgcagt tgccgcgcac agcgataacc ttatgtgcca cttcattaag
    12421 ccgttcagcg actttggctg gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt
    12481 caacacgtcg ccaaggatca ccagccactg ggcaccgctt tgggcaaaca actccagaac
    12541 acgttccgtc gccggtaacg acccatgaat gtccgatgca aacatcagtt tcatcactca
    12601 ctcctcgtcg aaaaaacgta agctatgata ctggattctg tcgttgctat cagccagatt
    12661 gcttcgccga aagtgcaata aaacgctgga cagacgctcg ctgccactgg aatgttgcat
    12721 aatccaccag gcgttctggc acctcatcgc catgtagtac caggcggtta atcattagcg
    12781 ccagatcagt atcagcaatg caccattcac caaataaatt cggctgaccc agtactaaca
    12841 aatgttctgc catcgcgaac agtttctctg cactggcttt tccctcggcc gttagtggcg
    12901 cttttttcgc ccccgcaaag acaacatccg tcggacgctc ttcgcggatg ggcatcagat
    12961 cgctgcgcaa ccaggcctga atctgtcgtg cacgcgcacg attttctaaa tcaagcggat
    13021 aaatacgttc ccaggtcggt ggcgcaaatc gatcttccag atactccgca atggcagaag
    13081 attcactcaa ctcaaaatca tcgatttgta ataacggcac acggcgtgtc tgaccgtaac
    13141 cttgccacgt cggttgcaaa tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg
    13201 acaggccttt ttcctgcaac gccacccagg cggataacac ataaggggag aaacagtggg
    13261 catctgacca aagcgtgata gcgggtttac tcattatgtc ctcggcaata aagggggctg
    13321 gctatcaaac tatagagcct ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc
    13381 gcgctttcta tacttgtggt gttcagatga ttagacaacc ggagctgcaa tgatcgatct
    13441 ctatttcgcc ccgacaccca atggccacaa aattacgctg tttctcgaag aagcaggtct
    13501 ggattatcgc ttgataaagg tagacctggg gaaaggcggt cagtttcgcc cggaattttt
    13561 gcgcatttcg cctaacaaca aaattccggc aattgttgat cattctccag ccgatggcgg
    13621 cgaaccgcta agcctctttg aatctggtgc cattttgttg tatctggctg agaaaacggg
    13681 gctctttttg agtcatgaaa cgcgtgaacg cgccgccaca ttacagtggt tattctggca
    13741 ggtaggcgga ctggggccga tgcttgggca aaatcatcat tttaatcacg cagcccccca
    13801 aaccattcct tacgctattg aacgttatca ggttgaaact cagcgtctgt accatgtact
    13861 gaacaagcgg ctggaaaact cgccctggct gggaggcgag aactacagca ttgcggatat
    13921 tgcctgctgg ccgtgggtta atgcctggac tcgccagcga attgacctcg caatgtatcc
    13981 ggcagtcaag aactggcatg agcggatccg ttcgcgccct gccaccgggc aggcactgct
    14041 aaaagcacaa ctcggtgatg agcgttcgga tagttaacag aaacaggttc tcgtgtatta
    14101 tttcatccta agtaaaacaa cggagaacct gcaatggcac aacctgccgc tattattcgt
    14161 ataaagaacc ttcgtttacg tacgtttatc ggaattaagg aagaagaaat taacaaccgt
    14221 caggatattg ttatcaatgt gacgatccac taccccgccg ataaagcgcg cactagcgaa
    14281 gatatcaacg atgcgctgaa ttatcgcacc gtaacgaaaa acattattca gcatgtagag
    14341 aataaccgtt tctctttgct ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa
    14401 catcactggg tgacgtatgc tgaagtggag atcgataaac tgcacgcgct gcgctacgcc
    14461 gattcggtat ccatgacctt aagctggcag cgttaaccgc catatcggga ggctgcatga
    14521 acatagtgat caccggaggg acgggattaa ttggtcgcca tttgattcca cgtttgctgg
    14581 agctgggcca tcaaatcacg gtaatgacgc gtaacccgca gaaagccagt tccgttctcg
    14641 gccctcgggt gacactatgg caagggcttg ccgatcaaag caacctcaac ggcgttgatg
    14701 cggtaatcaa cctggccgga gaaccgattg ctgataaacg ctggactcac gagcaaaaag
    14761 agcgtctctg ccaaagccgc tggaatatca cgcaaaaact ggtcgatttg attaatgcca
    14821 gcgacacgcc accgtcggta ctcatttccg gctcggcaac gggctattat ggcgatttag
    14881 gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga atttacccat aaactctgca
    14941 cccgctggga agaaattgcc tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc
    15001 gtaccggcgt agtgctggca ccggatggcg gtattctcgg taaaatgctg ccgccgtttc
    15061 gtcttggcct gggcgggccg attggttccg gtcggcagta tctggcctgg attcatatcg
    15121 atgatatggt caacggcatt ctctggctgc tggataacga gctgcgcggg ccatttaata
    15181 tggtttcgcc ttacccgata cgcaatgaac aatttgccca tgcgctcggt catgcgctgc
    15241 atcgcccggc tattttgcgc gtccccgcaa ccgccattcg gctgttaatg ggcgaatctt
    15301 cagtactggt attaggtgga caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg
    15361 cgtttcgttg gtacgattta gaagaggcgc tggcggatgt cgttcgctga tgtggtttac
    15421 tgcaaacatc cgccagttaa ctcccggtgt tacagggtta gtggctttgc gcgataagat
    15481 cgtctggtga aagtcgggtc agcgtcagga tgacttcgtg gtcaaatcct ttttccagca
    15541 tctcctgagc aatacgcaga gcttcttcgt gtttgccctg cattgcgcct tcttcacgta
    15601 atctgtcagc aatggtcatc agtttctcct tttcttgtgg tgcgcgttct gttatctcac
    15661 caataaatgc acggaaacgc tgggcatctc ccgtttgtaa tacataatta aacagggctt
    15721 ttagctgtct gtcattagtg ttccctgtaa ctagcagcga aacaatttgg tcgactaatc
    15781 ccaacagatc gcgctgacga atatgtttct gaattaactc caacagcgcc attttgcgat
    15841 gttgcataat ctcgtcatcc ggcaccacgg taatatccac caacggaaaa gccgatgaat
    15901 atattttacg ggcaatcgca ggctcggcaa attcatcaag ccagcagagt gaataaggat
    15961 aaggacttct gcaaccatga taaaacagca tcgggatcac caatggaagc tctttatagc
    16021 ccgcatcaag atggttttgc atcgccgcaa tggaataacg catcatgcga aaagccatta
    16081 attcttccgg cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg
    16141 ttttcacaga ccacaagagg tcggaataat attgccgcag gtcttcatca ataaaactgt
    16201 ttggttccag tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg gtaagatgaa
    16261 tatcaataaa atcccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat
    16321 gcggcgtgga agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac
    16381 gcgacaataa ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa
    16441 ttcagaagta tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat
    16501 tccccttgaa aatactccgg cagcgttagc caccggagag gcaattattt cagcgaacct
    16561 ttaaggaact gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgcct
    16621 tcttcctcta ttttcccctg atggaggaaa atgacgtgag tagaaacatg acgagcaaag
    16681 cccatttcgt gagtcactac caccatggtt ttcccctctt ctgccagctg ctgcataatg
    16741 cgcaacactt cgcctaccag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa
    16801 acttccggtt ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa
    16861 agatgcaccg gatatttccc ctgtgcacgt tcgtctatcc cgacttttgc cagatacttc
    16921 accgcccgct cgcgcgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg
    16981 acgttttcca gcaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg
    17041 cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg
    17101 cgcaccagat tgatcgtctg gccattgacc acgatcgccc cttcactcgg tttttcgagg
    17161 aagttaatgc agcgcagaaa ggtacttttc cccgatcccg acgatccaat gatgcttatt
    17221 acatctccgg cattcgcttg cagtgacacc cctttcagca cttcgtgttc gccatagcgt
    17281 ttgtgcaaat cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga
    17341 aggtttcaca tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata
    17401 agagatgatt aaatagagca ccgcggcaat gccgaaggca gtaaaaggtt gatacgtggc
    17461 ggcgttaata tcgcgggcta ttttcagcag atccggcacc gtggcagtaa atgccaacgc
    17521 agtagagtgc agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc
    17581 agaaggcaaa ataatgcagc gatacatttt aaaagtcgag aagccatagg ctctggcggc
    17641 ttcaatttcc ccatgcggaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc
    17701 gcaggtgtta agcgtcagcg ccagcacggt acagttcagg ccactgcgga aaaaggcgtt
    17761 aagaaactcg gtccccttaa caatctccag cgtatacatg ccggaataga acaccagcaa
    17821 ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat
    17881 gtatttatta ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac
    17941 taccgacaaa ataagcagcc acagggtgat cgccacacca gtaaagcgat aaccgtcggt
    18001 ccacagcagc ggtttccagt attcatgtaa gatttcgatc acagatcagc cctcttcaca
    18061 cccacggagt agcggcgctc aaggaacagc agcacaccat tggaaacggt ggtgaaaacc
    18121 aagtaaatca cgccacagac gatggcgaaa tagaacggtt cccaggtact tttgcctgcc
    18181 agctgcgtgg ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg
    18241 aggatcacct gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac
    18301 atgatccgcc gaaacacttg cccacgagta aaaccgaacg ccgtcgccgc ctctatatgt
    18361 cctttcggca ctgccataaa agcaccacgg aacgtttcgg taaaataagc accgtagata
    18421 aaaccgagag tgataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc
    18481 gcctccgtca ccgtgtttag cgcaatctgc aaaccgtaga aaatcagcag catcaacact
    18541 aagtccggca cgccacgaat cagcgtggtg tacccttcga aaatcagccc cgaaagccga
    18601 ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag
    18661 ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac
    18721 aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac
    18781 ccgaagggag cgggctttct ttcaccgatg gcgattaacc accataaaca tcaaaatcga
    18841 agtacttttt cgctaatttc tcgtaagtac cgtcagctcg catttcggca aaggctttgt
    18901 tcagtgcctc gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa
    18961 acagtttttc atctttaaca gacgggccac cgaatttgta atctttaccg acaggttgtt
    19021 tgaggaaacc ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag
    19081 tcaggtcaga ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg
    19141 cccagtgttc gttaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc
    19201 ctttcagcga ctcgactgtc ggctgaatgt cagaattttt cgccaccacc agacgagaat
    19261 cggcagcgta cagtttgtcg gtgaaggcta tttcttgctg acgtttttcc gtaatggaaa
    19321 gcgatgacat gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg
    19381 gattttcgac aaacgtacat tgcgtattga tgcgtttgca taattccttt gccagatcga
    19441 tatcgaagcc aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt
    19501 cggtaccgat gcggattttt tgcggaatcg cagcaaatgc cgcagttgcg ctggagaagg
    19561 ccagaaccag agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt
    19621 ttatagggaa tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc
    19681 aaaatatctc gccccgttag acgtattatt tttcattaaa aatgcttaag tatgcatttc
    19741 aatgcaccac gatagtgcaa caaacctggc gtgcaccatg atgatcattc catcaggtac
    19801 agcttccctg cgacgtatca gtcaccgtag acattaaagt caaaatactt tttcgccatc
    19861 ttgtcgtagg tgccgtcctg acgcagctct ccaagcgcct tattgaaagc agccgtcagt
    19921 tcagcatcat ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttc
    19981 actgatgagc cagcaaaggc gaagtcttta ccagcaggtt gcttgaggaa tccttcgctg
    20041 gcagcaactt catcctgcag tgcagcatcc agacgtcctg cagccagatc ggaatagacc
    20101 aaatcctggt tggcataggc caccacatcc acacctttgc tacgccaggt ctcgttagcg
    20161 taagcttcct gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc
    20221 gttggctgaa tcggcgaacc tttggcggca atcagacgag aatctgcggc gtacagcttg
    20281 tcggagaagg caatctcctg ctgacgttta tcggtaatgg aaagcgacga aataatggcg
    20341 tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa agtcactggc aacccaggta
    20401 catttcacct gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa
    20461 tcacctttag catctttcga cgagaacggc gcataggtgg tatcggttcc gatacgtacc
    20521 gtctccggta gcgccgcata gctggaagcc gctgcggaga gaccgactaa caaagacaga
    20581 gcgagaatcg acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta
    20641 ttgtgttgtg tgtttgcagg ctatttcatg caggtcttat gccatcttga cgacaacagt
    20701 aacattcaac gttaaatgtg ttaataagac gttgcattat tgtcctgaag ttgaagatag
    20761 caggtatagc ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat
    20821 ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca
    20881 gcgggcaaag agatcttcag gaagggttat cgcaaactgg tcaagaacac gattaaccgt
    20941 ctgatttatc acatcatcaa gggattgcgg gcgatgataa aacgccggaa cgggaggcat
    21001 aatcaccgca ccgatttctg ctgcctgagt cattaaacgc agatgaccta agtgcaatgg
    21061 tgtttcacgc acgcagagca ccaacgggcg acgctctttc agcacaacat ctgccgcacg
    21121 ggtcagtaag ccatcagtat agctatggac aatgccggac agggttttga ttgaacaggg
    21181 taaaatcacc atccccagcg tctggaaaga accggaagag atgctggcgg caatatcgcg
    21241 cgcatcatgc gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag
    21301 ggataaggtc tggcgcgccg cctggctcat caccagatgc gtttcgatat ctgtgacatc
    21361 gcgcagaacc tgtaataaac gcacgccata aatcgcgccg ctggcaccgc tgatgcctac
    21421 aatgagtcgt ttcataaata gttgccctgt cagacttgca ggcagacttt gcaggatttc
    21481 ggcgggagtt gcaagtcagg gtgccagacc ggcaccctga gcgaaggcat catccttcgt
    21541 tatgcatttc gagattttcc acttcgttct gacgttgcac tgctttggcg tcgtcattac
    21601 gtaacgtatc gaggaaatcg aggtagccct gatcaacatc tttggtgacg tagacgccgt
    21661 tgaacaccga acattcaaac tgctgaatat ccgggttttc agcgcgaacg gcttcgatca
    21721 gatcgttcag atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcta
    21781 cttcgcgacc gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg
    21841 ggaagcgaat ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg
    21901 ccatctcgat aatctgctca gaagtggtgc cacggacgat ggagtcgtcg accagcagga
    21961 cgtttttatc gcggaactcg gcgcggttgg cgttcagttt acggcgcacg gacttacgac
    22021 gcagctgctg gcccggcatg atgaaggtgc ggccaacata gcggttttta acgaagccct
    22081 ggcggtacgg cttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttccg
    22141 ggatcgggat caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcgccca
    22201 gtttcgtgcc catattcaca cgcgcgctgt aaacggaaat tttgtcgatg aacgagtccg
    22261 ggcgagcaaa gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt
    22321 gacgggtaaa caactgccct tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac
    22381 gcaggaaatc aaatcccagc gtatcgagcg caacgctttc ggaagcgacc atatattctg
    22441 tacggttctc gtcaatatca cgttttccca gtaccagcgg acgaataccg tttggatcgc
    22501 ggaaagcaac cataccgtgg ccgataatca tcgccacaca agcatacgcg ccgcggatta
    22561 agcggtttgt agcagcgatg gcggcgaaaa tgttgtcggc ttccagcggg tagtggcgga
    22621 agttgtccag ctcgctggcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga
    22681 tgtggcggcg tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc
    22741 cgttgtgggc aagcgtaatg ccatacgggg agttaacgta aaacggctgc gcttcagagg
    22801 cgctggagct gccagccgtg gggtaacgca catgaccaat gcccatattg ccctgcaaac
    22861 gctgcatatg gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga
    22921 agcagttatt ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca
    22981 ccgttaaggc atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac
    23041 cgcacatacg tctttttcct cgttaagcca catctcagag cacttacgct ctgggcaaga
    23101 aacttgacga gctttgcaga taatcaaaaa accatctgat gataaaactg aactgcggga
    23161 tcagctgtga tttactccag tcttcgcttt tcgacacccc ggtaaaggag tcgagaaaga
    23221 agagaatggc agcaacaatc aacacaccgc gcaacgcacc gaaacagacg cccagcaccc
    23281 gatcggtgcc tgacaacccc gttttctcca ccagctggcc tatcacgaag ttcacgatag
    23341 caccaacgat cagggtagcg ataaacagta ccgcgatggc aatcccattt cgaaccagtt
    23401 cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga
    23461 aagcacaacc ccatgtcacc agcgataacg cttcacgaac aaagccgcgg atcaggctaa
    23521 ccagagagga aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc
    23581 cacgatttta cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt
    23641 gcgtagggat ttccttcccg cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca
    23701 tcgacttttt atgcctttgc ggcatcggac aatgcgtgtc ggatgcggcg taaacgcctt
    23761 atccgaccta cggttctaac cctgcgtagg cctgataaga cgcgccagcg tcgcatcagg
    23821 caacggctgt cggatgcggc gtgaatgcct tatccgacct acggttctac ccctgcgtag
    23881 gcctgataag acgcaccagc gtcgcatcag gcaacggctg tcggatgcgg cgtgaacgcc
    23941 ttatccgacc tacggttcta cccctgcgta ggcctgataa gacgcgccag cgtcgcatca
    24001 ggcaagaccg tattaattcg gcgtatagcc cattaccacg ccacttaagc cagaaagttg
    24061 cttcaactca cccagcgatc ctttcagctt atctttcgag gcatctggcc caaccagaat
    24121 acgggtaatt ttaccctgca ctggcgtgga tggcgacgta taaacccgat aaccggcacc
    24181 gcgcagctta ccgacaatct cattcacttt atcggcattt ttcagcgccc ccagttgcac
    24241 aacataggct ttacccgtcg gtgcagcttt ttcttccacg accggcttcg gttctggctt
    24301 cggcgcaggt ggtgcttcaa cctttggttt aggcggctcc accggtttcg gcttcggtgg
    24361 ggcgaccggt gcaggttccg gttcaaactc ggtgttattg gctgcaatag tggccggatc
    24421 gagcgacggt gctgcggcat cacctgcccg cacctcttcc gctgcgcctt ccggcggttg
    24481 cgtcggcaac gcctgggtgg cggctggcat catatcaggc tcatcgcgat cgcccgcttt
    24541 tggcaccagc gggatagccg caaactcatc ctgataatgt tttttctgcc cgtccagcag
    24601 ccctggaagt acaatcaccc ccagcgccac cagcacgatc gtgcccacta accgattctg
    24661 aaacttactt gccaccgctt ctcctcgcgt caatcacttc catgacatgt gcgaccgtgt
    24721 ggaacgaacc acacaccagc acggtgtctt ccgctttagc gtccgccatt gcggcatccc
    24781 atgcctgcgc aacgctatca aatgatttgc cgttacccaa atgctcaagc agttgttctg
    24841 ccgtggcacc gcgcggccct tccagtggcg cacaatacca gtcatcaacc acgcttttca
    24901 accaggccag agttccggca atatctttat catgtagcat accgataacc gccagcacgc
    24961 gcccgttttt cggtagcgtt ttcatacgcc cggtgagata ttccgccgca tgtggattat
    25021 gcgcgacatc aaaaataacg cgtggcgact cgctcacaat ctggaaacgt cccggcaaaa
    25081 ttgcgctggc aatcccgtcg cgaatggcat tttcactgac ctccagcccg ctggcacgca
    25141 gtgccgccag cgccgttgcg gcattcggtt gcgggacaag cggcaacggc aggttttcca
    25201 gcgtgccgtg agcatcgcta aacgcccagt catgatcggt gacggaatag ttccactcaa
    25261 cgccccgacg ttgtaacagt gcaccttttt cctgcgctac atcagcaatg gtagaaggca
    25321 tttccggctc accgacaatt gccggttttt cgctgcgaaa gatgcctgct ttctcgcgac
    25381 caatactttc gcgatctggc cccagccagt cggtatgatc cagcgcaatg ctggttacta
    25441 ccgcgacatc ggcgtcgaca atattggttg cgtccagacg accgcccagc cctacttcca
    25501 gaatcaccac gtcaagttgt gcctgtttga acagccacaa cgccgacagc gtaccgtact
    25561 cgaaataggt cagggaaata tcaccgcgtg ccgattcaat ctccgcaaaa gaggcagtgt
    25621 gggccgattc cggcaattcc tgaccctgca cacgtacgcg ctcggtataa cgcaccagat
    25681 gcggcgaact gtagacgccc actttgtacc ctgccgccat cagaatcgac tccagcgtac
    25741 ggcaggtggt gcctttgcca ttcgtacccg caacggtaaa cacaaatggc gctggtttca
    25801 gaacgccaag acgcgccgcg acctggctca cgcgctcaag gccgagatcg atagttttac
    25861 tgtgcaggtt ttccagataa gaaagccacg aagccagagg cgacgcggct tgaggagtgc
    25921 gtttgataat catggtatcc gctgattcgt tacggtgaga atagcaaaag ggcagagcca
    25981 gtggccctgc ccttatcagt tatcaggcct caggttcctg atccggtacc gggggtacca
    26041 ctacgccttc acgcggcgct tcaggattcg gcgctggcag attcatcaac ttcgccagaa
    26101 tgctcgccag tttcaggcgc atttccggac gacggacgat catgtcgatc gcgcctttct
    26161 cgatcaggaa ttcactgcgc tggaatcctg gcggcagttt ttcgcgaacg gtctgttcga
    26221 taacacgcgg accggcaaag ccgattaacg ctttaggttc agcgatgttg agatcgccca
    26281 gcatggcgaa acttgcagaa acaccgccca tcgtcgggtc ggtcagcacg gagatgtacg
    26341 gcaagccgcg ctcctgcatt tttgccagtg ccgcagaggt tttcgccatc tgcatcagcg
    26401 acatcagtgc ttcctgcata cgtgcgccac cagaggcgga gaagcagatc agtgggcagt
    26461 tatcttccag cgcctgctca acggcacgca cgaaacgtgc acccacaaca gaccccattg
    26521 aaccgcccat aaaggcgaac tcgaatgccg cagcgacaac cggcattcca tacagagtgc
    26581 ctttcatcac caccagcgca tctttttcgc cggtttcttt ctgcgcagat gccagacggt
    26641 ctttatactt cttggagtca cgaaacttca gcacatcttt cggctcaagc tcgctaccca
    26701 gctccacaag gcttccttca tctaacaggc tatgcaggcg attacgcgct gtcatacgca
    26761 tgtgatggtc acacttcgga cagacctcaa gattacgttc cagctcagcg cggtataaaa
    26821 cctgaccgca gctatcacac ttagtccaca ccccttcagg aatgctcgcc ttgcgggtgg
    26881 gagtaatgtt gcttttaatt cgttcaatcc agctcattag ggacctttct gtctgaacct
    26941 ggttcgatgc cagttttatc tttggggacg cataatgcca tttttgcccc caacagacca
    27001 tgaatgttgc acattaaaac ataacagccc gaaactttgg ataaaaaagt ggtcgaaccg
    27061 cggagttact ttttattttg cggcgcgtgc cgcagcgcgt ttgtgacgga ttatttcgat
    27121 gacgcccggc aaaatagaaa ccacaataat cccgacgatc agcagcttaa ggttatcctg
    27181 aaccatcggg attgtaccga agaaatagcc tgcgtaggta aaaagcagta cccacaacag
    27241 tgcgccgatc acgttataag cggcgaaatg acggtacgac atgtggccca ttcccgcaac
    27301 aaacggggcg aacgttctga cgatcggcac aaaacgggcg agaataatcg ttttgccgcc
    27361 atgtttctca taaaactgat gggttttgtc gagataactg cgacggaaaa ttttcgaatt
    27421 gggattactg aataactttt caccgaacaa ccgcccgata gtgtaattga ccgcgtcacc
    27481 cacaatcgcg gcgatcagca tcagcaccac catcatatgg acgttgagat cgttggtttc
    27541 cagtgatgcc aacgctccgg caacaaacag caatgaatca cccggtaaaa acggcgttac
    27601 caccagaccg gtttcacaga acaaaattaa gaacaaaatg gcataaaccc agacgccgta
    27661 ctccgcgacc agctctgcca gatgcacgtc aatgtgcagg ataaaatcga tgaggaaata
    27721 aatcaggtcc atatttgcct atgccttgta ctcgtcatct ttcaggctgt aactacgttg
    27781 gctacagcct gaaatgttcc gagcattatt ctcgttagtc cgccagaaat agcgggccca
    27841 ttggcggttt tggaagatca aaccggtcag ggtaatccac cgcgaccaga tacagccctt
    27901 ccgcttttgc cgttgctgcc gccagcgttc tgtcctttgc cgccagcaac tctgctatcc
    27961 agctctccgg ctggttgtgg gcaccgactt ccatcaggct gccgacaata ttcctgacca
    28021 tatgatgtac aaaggcattc gctttgatat caaccaccac ataaggaccg tgacgcgtga
    28081 cattaatatg cataacgttg cgccacgggg ttcgggactg gcactgcacc gcacggaacg
    28141 aggtgaaatc attctcgccc agcaagcatt gcgcagcccg atgcatccgt tcagcgtcca
    28201 gcggttcgta aaaatgggtt acccctttac tcagtaccgc cgggcgcagc cgatgattgt
    28261 agatgatata gcgataacgg cgagccgtgg cgctaaatcg ggcatgaaaa tcatcaggta
    28321 cagctttaac ccaacgcacg gcgatgtcac caggtaaatt cgcatttacg cccaacgtcc
    28381 acgcagcgtc tttgcgctgc gcggttgttt cgaaatgcac cacctgcccg gtaccgtgta
    28441 cccctgcgtc agtacgcccg gcgcagaaga cggtgatggg ttcgttcgcc acctgggaga
    28501 gcgccttttc cagcttctcc tgcacactgc gaacttcgtt ctgccgttgc cagccgtaat
    28561 acttactgcc gtcgtactca atgcccagcg caattttata aactggcggt tgttgctggt
    28621 cggacattag tacagatact cctgcaccag tttctcggcg atttttactg ccatcagcgc
    28681 gccgccaaag cgaacgttat cggccaccga ccagaactgg acttgctccg gcataccgta
    28741 gtcattacgc acgcagccaa cagaaagatg cggcgtaccc gaagcatccc ctacctgagt
    28801 tgggaattcg ttctcttcag agagcacaat atcttcgcct tgaacaaacg catcacgcgc
    28861 ttcttctgct gccagtggac gcagagcttc aaagttgacc atctgggcat gaccgtagaa
    28921 taccggtgcc tggacgacgc ttgccgaaat catcagtcct tcgtcctgca ggattttgcg
    28981 tacttcgtca acgatacgac gttcttcacg cacgctacct tcgctatccg gcagtaacgg
    29041 cagcatgttg aacgccagct gacgcccgaa gaaatcttct tcgtcaatcg gaatgccgtt
    29101 gagcaattta gcactctgcc ccgctaacgc atcgaccgct tttttgccct gggcggaggc
    29161 tgaaatcagg ctggtaacgc tgatacgtga taaaccgccc tgatcgatta acggtttcag
    29221 tgccgccagc agctggctgg tcagactgtc cggtacggcg atgacattcc ggttgcggta
    29281 atcggtcagt acaaacgggt ttacttccgg caccaccagc ggtacgtcgg gttcgagagc
    29341 aaacaatccg ctgctgtcga tcaccaggca acctgagttg gtcgcttctt caacccaggc
    29401 tgcggtagct tctttgcctg cgacaaaaaa tgccagctgc gcctgcgtcc agtcgaattc
    29461 agcagcatcc tgcacggtga ttgtcttacc accaaagcgc agttgttcgc ctgcgctttc
    29521 gttacgtgcc agtgcataaa tttccccaac cgggaactga cgttcagcca gcgtttcaag
    29581 cagggcttcg cccacagcgc cagttgcgcc caggacggca atgttccagc cttcagacat
    29641 ggtggtttac tccagaaata gcaaagctcc ctgccaatgt tacagcagag agcatgaaga
    29701 agagattaac gtgccggatg atgaacggcg ttaaaaccca gtttacacag caatgatgcc
    29761 gcactggcgt catcacaaat tacatacaga gatgaccatt cacggcgctc aagatagttt
    29821 ttgcgcagtt tatcgaactc acccggtatc ccggcgactt tacgcagcgg tgcgtcatcg
    29881 cggcgcacat catacaccaa atgcaccagc cttttcagcg ttggttgatc gagcgggcca
    29941 tgcagcgtaa tgcgaccaaa ctctggcgcg ggcagtaatg tatccagcgc aatgtgctgt
    30001 tcatgcccaa taaacttgct ataggcttca aacacttgcg tagtaccgcg tgctttacct
    30061 tccagggtat agcctgcaat atgcggcgtg ccgatatcca cttttgtcag caactccacg
    30121 ttaagttccg gttcgccttc ccagacatcc agcactacgc ttaacttctg gccttcattc
    30181 aggcaggtca gcagcgcggt attatcgacg actgcgccac ggcaggcgtt aatcagaatc
    30241 gctccgggct tcaggttacg gatcagtttt tcatccgcca gatgcagcgt tttgtacggc
    30301 ccgtctttga agagtggcgt atggaaagtc agaatatccg catgctgaac taattcatcc
    30361 agcgagcgga aatcaccctc atccccacgg tcagcgcgag gcggatcgca aagcaaggtt
    30421 ttaatcccta acgcttccag tcgcgcctgc aatcgacgtc caacgttacc aacgcccacg
    30481 atccccaccg tacggtcgtg cagtgaaaat ccatcgcgtt cggcaagcat cagtagtgag
    30541 gagagaacat attccaccac cgcaatcgca ttacagccag gtgcagcgga aaaaccaatt
    30601 cccgccttct ttaaccatgc ttcatcgaca tggtctgtcc ccgcagtggc agtgccaaca
    30661 aatttaatgg gttttcctgc cagcaaagat tcattcactt tcgtgaccga acgcaccatc
    30721 agcgcatccg cgtctgccag ttgagcgacg gggattggac gcccgggaac cgcgatcacc
    30781 tcacccaaac ggctaaataa gtcgcgggca taaggcatat tttcatcaac aaggattttc
    30841 acgtttgtgt tacctgtatg agacgagagt taaccggaca agtgtgccat aatctcgcgg
    30901 ccaggcatac ttgcgaagat ttcaggtata aggatacgta atgatacaac ctatttccgg
    30961 ccctcctcct gggcaaccac caggtcaggg agataacctg ccgtctggcg cgggcaatca
    31021 gcctttatcc agtcagcaac gtacttcgct ggaaagctta atgacgaaag tgacctcact
    31081 gacgcaacag caaagagcag aactgtgggc gggtatcagg cacgatattg gtctgtcggg
    31141 agattcaccg ctgctttcgc gtcacttccc tgccgctgag cataatctgg cgcaacgtct
    31201 gctggccgcg caaaaaagcc attctgcccg ccagctttta gcgcaattag gggagtattt
    31261 acgtctgggg aataatcgtc aggcggtcac ggattatatc cgtcataact ttggtcagac
    31321 gccgctgaat cagctctcac cggagcaatt aaaaaccatt ctcaccctgt tgcaggaagg
    31381 caagatggtt attccgcaac cgcagcagcg cgaggcgacc gatcgtcctc tattaccggc
    31441 ggagcacaat gcgctcaaac agctggtgac caaacttgcg gcggcaacgg gggagcccag
    31501 caaacagatc tggcaatcga tgctggaact ttccggggtg aaagatggcg agttaattcc
    31561 agcgaaactg tttaaccatc tggtgacctg gctgcaggcg cgtcagacgc taagccagca
    31621 aaatacgccg acgctggaat cactacaaat gacgctaaaa caacctttag atgccagtga
    31681 actggcggcg ttatcggcat atatccagca aaaatatggc ctttctgcgc aatcatcgct
    31741 ttcttctgcc caggccgagg atattcttaa tcagctttat caacggcggg ttaaagggat
    31801 tgatccgcgt gatatgcaac cgctgcttaa tccttttcca ccgatgatgg acacgttgca
    31861 aaatatggca acgcgtcccg cgctgtggat actgttagtc gcgattatcc tgatgctggt
    31921 ctggctggtt cgttaacccc gacgaaacga cagtatcgtg acaataattc ccagcaccgc
    31981 agagatcgcc ccggcaagaa ataccgaaga gtaaccaaac gtggtcgcca gcattcccgc
    32041 cagcggcccg gagacgccga gggcgatatc ctgaaacgcg gcgtaaccgc ccagtgcggt
    32101 gccgcgaact tgtgagggga cacgtttaac cacctccacg cccagcgcag gaaagataag
    32161 cgaacatccg gctccggtta acgccgcgcc cgctaatgcg acccacgcac ccggggcttg
    32221 ccagagcagc aacaagccca ccgtttctac aagcagagag acgatcgcca ctttcacgcc
    32281 gccaaaacgg tccggcatcc agccaaacat gacgcgcatc acgacaaatg cgccgccaaa
    32341 cgcggtaaga gtaaagcccg ccatcgccca tcctttgctg gcaaagtaga gcgaaacgaa
    32401 agtcccgata accgcaaaac caacgccttg tagtgccagc cctaaccctg gtttccagat
    32461 aagcccgaca acgctccaca gcgatggacg ttctcccgcc agggccggta ctttgcgcac
    32521 tgtgccgtta caggcccacg ccagtaaggg taatgccatt gtggtgagcg ccagtgcggc
    32581 aaaaccgtaa tggctatgaa tcaacaggcc aagcggtgca ccaacagcga gggcaccgta
    32641 aatcgccatt ccgttccatg acattacttt gccagagtgt tttggcccta cgatgcctaa
    32701 tccccaggtc agagcgcctg tcagtaactg gctttcacca aagccaagaa tcaaacgccc
    32761 gacgaccaac agggcaaatt tgaacggtgc ggagacgggc aaaatcgccg ccagcagcaa
    32821 cgcgcccccc gccagaccac aagctaacat cccctgaagc gccgaacgtt ttgcgccata
    32881 ttgatcggcc agtcgcccgg cgtaaccacg cgtcagcacc gtagccagaa actgaatgcc
    32941 gacggcaatg ccgaccatgg tattgccata gcccaattca tgatgaacaa acaacgggat
    33001 aaccggcaac ggcaacccta cggtcatgta ggtgagaaaa accgcaaaag cgatgcggaa
    33061 gagcgaaaaa ttggcagaag atcgtgtttc ggtttggctt acagcagtca tgcattactc
    33121 cagaatgcag cgcaaggcga ggagtatccc cgtctcatct ctctggtttc agggttacgg
    33181 tgcgttggca ggaattaacg cgtacgtctt ttcagaagga aatcgacaaa gcgggaagtt
    33241 tgcctggaac aggcggcgat tgtcaatgat gtgaaaaagg gaaccatcgg gttccctttg
    33301 gcgttagtgc cggatgccgc attggcgcgt aacgtcggat gcgacgctgg cgcgtaacgt
    33361 cggatgcgac gctggcgcgt cttatccgac ctactccgac tactccggcc tactgcgaat
    33421 taatctttca gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc gaagctgtta
    33481 gacataacag tggtcagttc gcgatcggtc gtttcggtca cgatgttcag acccgcagcc
    33541 tgctcgtcca gctcttcaat gttgatgctc ggggcgataa agccgtgttc cagcatcagc
    33601 agagagtaga tagcttcctg tacgccagca gcgcccagag agtgaccggt catggctttg
    33661 gttgcagaaa tcgccgggct cttatcgccg aacacttcac ggatagctgc cagctccttc
    33721 acgtcgccaa ccggagtcga agtgccgtgg gagttcaggt aatcgattgg ggtatcaacg
    33781 ccatgcatcg ccatctgcat acaacgtact gcgccttcgc cagacggagc aaccatgtct
    33841 gcgccatcag acgttgcgcc gtagccaacg atttcagcat agatgtgagc accacgcgcc
    33901 agcgcgtgtt ccagctcttc aaccaccacc ataccgccgc cgccagcgat aacgaaaccg
    33961 tcacggtgag catcgtaagt acgggaggct ttttccgggg tgtcgttgta tttagtagac
    34021 agcgcaccca ttgcgtcgaa ttcgcaagcc atttcccagc acagctcttc gccgccgcca
    34081 gcaaacacga tgtcctgttt gcccagttgg atctgctcta ctgcgttacc gatacagtgt
    34141 gcggaagtcg cacacgcgga gctgatggag tagttaacgc catgaatttt aaacggggtg
    34201 gcgaggcagg cagaaacgcc ggatgccatc gctttggtga ccacatacgg gccaaccgct
    34261 ttcaggccgc gcgggccgcg cattgcgtca gcgccgaaca cctggaaacg cggggagccg
    34321 ccgccggaac ctgcaatcag gccaacgcgc gggttattct ggtaagcttc cggagagagg
    34381 cccgcatcag cgattgcctg ctccatagaa aggaatgcat aaatggatgc gtcgctcata
    34441 aagcgcacaa ctttgcggtc aatgaggcca gtggtatcca gttttacgtt gccccagacg
    34501 tggctacgca tgccggaatc cttcagctcc tgagagaaag tgatccctga acgtccttca
    34561 cgcagagatg ccaggacttc ctgctggtta ttaccgatgc tggaaacaat gcccaggcca
    34621 gtaatcactg cacgtttcat tcaatacctc tgtaagtcgc acatagagta agtttcgaat
    34681 gcacaatagc gtacacttgt acgccgaaca agtccgatca gccaattaat agagaaattt
    34741 gcgcagcctt acacacatcg ctaagatcga gccaccgcct gtaagacgag taacttacgt
    34801 gaaacactac tccatacaac ctgccaacct cgaatttaat gctgagggta cacctgtttc
    34861 ccgagatttt gacgatgtct atttttccaa cgataacggg ctggaagaga cgcgttatgt
    34921 ttttctcgga ggtaaccaat tagaggcacg ctttcctgag catctacatc ctctgtttgt
    34981 ggtagcagag agcggcttcg gcaccggatt aaacttcctg acgctatggc aggcatttga
    35041 tcagtttcgc gaagcgcatc cgcaagcgca attacaacgc ttacatttca ttagttttga
    35101 gaaatttccc ctcacccgtg cggatttagc cttagcgcat caacactggc cggaactggc
    35161 tccgtgggca gaacaacttc aggcgcagtg gccaatgccc ttgcccggtt gccatcgttt
    35221 attacttgat gaaggccgcg tgacgctgga tttatggttt ggcgatatta atgaactgat
    35281 cagccaactg gacgattctc taaatcaaaa agtagatgcc tggtttctgg acggctttgc
    35341 gccagcgaaa aacccggata tgtggacgca aaatctgttt aacgccatgg caaggttggc
    35401 gcgtccaggc ggcacgctgg cgacatttac gtctgccggt tttgtccgcc gcggtttgca
    35461 ggaagccgga ttcacgatgc aaaaacgtaa gggctttgga cgcaaacggg aaatgctttg
    35521 tggggtgatg gaacagacat taccactccc ctgctccacg ccgtggttta atcgtacggg
    35581 cagcagcaaa cgggaagtag cgattatcgg cggtggtatt gccagcgcat tgttgtcgct
    35641 ggcgctattg cgtcgcggct ggcaagtaac gctttattgc gcggatgaag tccccgcgct
    35701 gggtgcttcc ggcaatcgcc agggggcgtt gtatccgtta ttaagcaaac acgatgaggc
    35761 gctaaaccgc tttttctcta atgggtttac ttttgctcgt cggttttacg accaattacc
    35821 cgttaaattt gatcatgact ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca
    35881 gcataaaatc gcacagatgc tgtcaatgga tttacccgaa gaactggctg tagccgttga
    35941 ggcaaatgcg gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggca ttacttatcc
    36001 gcaaggtggt tggctgtgcc cagcagaact gacccgtaat gtgctgaaac tggcgcaaca
    36061 gcagggtttg cagatttatt atcaatatca gttacaggat ttatcccgta aggatgactg
    36121 ttggttgttg aattttgcag gagatcagca agcaacacac agcgtagtgg tactggcgaa
    36181 cgggcatcaa atcagccgtt tcagccaaac atcatctctg ccagtatatt cggttgccgg
    36241 tcaggtcagc catattccga cgacaccgga actggcaaaa ctgaagcagg tgctgtgcta
    36301 tgacggttat ctcacgccac aaaatccggc gaatcagcat cattgtattg gtgccagtta
    36361 tcatcgcggc agcgaagata cggcgtacag tgaggacgat cagcagcaga atcgccagcg
    36421 gttgattgat tgtttcccac aggcacagtg ggcaaaagag gttgatatca gtgagaaaga
    36481 ggcgcgctgc ggtgtgcgtt gtgccacccg cgatcatctg ccaatggtag gtaatgttcc
    36541 cgattatgag gcaacactcg tggaatatgc gtcgctggtg gagcagaaag ataaggcggt
    36601 aagcgcgccg gtttatgacg atctctttat gcttgatgct ttgggttcgc gtggtttgtg
    36661 ttctgcccca ctgtgcgcag agattctggc ggcacagatg agtgaagaac cgattccgat
    36721 ggatgccagt acgctggcgg cgttaaatcc gaatcggtta tgggtgcgga aattgttgaa
    36781 gggtaaagca gttaaggctg ggtaaccagc tttggcattg tttgtcggat gcggcgtgaa
    36841 cgccttatcc gacctaagtg tgacgtgtag gcctgataag acgcgataag cgtcgcatca
    36901 ggcatggtgc tccagacgcc gcaacttact gctgtgacgc ctgctgaaac aggttttccc
    36961 acatatcggt caccagcgcc tggtcacgcg gcgacaattc accagcacca atggcttttt
    37021 ccagactctg gctaacggtc gtatgcaccg cctgagcgga gtggtcatca ccactttcca
    37081 gttctgcgat ggctaatgtc aggtggccac gcaaataccc actggcaaac aactcatcat
    37141 cactggcatg gtcaaccata ccgtcgatta atgccagaat gcgtgattca aactccgcga
    37201 tcatcttctt tcctcattgt aaaacgtggc gctggcttca gttgagcgct tccggccacg
    37261 ggaactgttc cgccgtaagt tccggcgtgt gataataatt ttgtagtgct ttaatgaaac
    37321 gtgccgggcg ttccggaatg ccatttgtta gataatccat cacctgcgca tgtactcgcc
    37381 gttgaaacac cacgcggtcc ggttcgaagt cgccttccag attgtcgcaa ctgacgttaa
    37441 acggatatcc agccgccacg cagaacaacc agtcgagcgc ctgcggcttc acttcaacat
    37501 cttcaaactg gctttgcgtt tgggcatcgc gtccgtccgg gcaataccag tagccgaaat
    37561 caaccagctc acggcgcgct tttccggcaa tacaccagtg cgaaatctca tgaattgcgc
    37621 tggcataaaa gccatgagca aagacgattc ggttatacgg tacttccgca tcagcaggaa
    37681 gatagatcgg ttcgtcgtcg cctttaatca gacgggtatt aaaatcatcg gcaaagcagc
    37741 tattaaaaat ttcaatcaac tgctcatagt ggtgtgtact gttcattagt tcatccccaa
    37801 ccagtggagg atctcctgtc cgtggctatc gtaaagtaat ttggcactca tcaccgcgga
    37861 gacgataaca atcatcgggc ggatcagctt ttgtcctttg ctcaacacca gtcgtgaacc
    37921 catgcgcgcg ccgaggaact gccccaccag catcacaaag cccgtcgccc aaatcacttt
    37981 gccgccgaga ataaacagca gcaaaccacc gatgtttgac gttgcgttga gtaatttggc
    38041 gtgagccgtg gctttggcga ggttgaatcc gcacagcgta acaaaggcca gcgcgtaaaa
    38101 cgatccggct gccggaccaa agaatccgtc ataaaaaccg acgcagccac cggcaattaa
    38161 cgcgaagggt aaaccgtaca tccggcgctg gcgatcttct tcacccagct ttggcatcag
    38221 caagaaatag aggccgatac aaatcaccag aatgggcaaa atctgccgta agacatcagc
    38281 ctgaacgtac tgtaccagca gcgcgccgct cattgagccg acaaaggtca tggcgatatt
    38341 gagtttctga tcgcttaaac taaccacttt gcggcgaata aagtagatag tggcggaaat
    38401 agagccgccg caggcttgca gtttattggt tgccagcgca ttagcgggag acatccccgc
    38461 cgccatcaat gccggaatgg tgagtaaccc accgccaccg gcaatcgagt cgataaatcc
    38521 cgccagcatg gcgacaaaaa agagaactcc cagcaacagc ggggaaacca taaacaggct
    38581 attaaacgtt tccattagat cacgtgctca tccagtagcg cctggcagga aggcggcaac
    38641 ggaggcggtg tcttcttctc aggctttgtt gttcccggtt ttggaggttc aaaccagctt
    38701 tgcagttctg ccccgcaacc atcgcctggt ggcggtaaag gttgatcttc acactccaga
    38761 ctatcggcag gacaacgtaa tcgtacatgc atatgcgcac gatgctggaa ccagggtcgc
    38821 actttgcgca accagtcgcg atcggtgccc gcatcaaggc aaagttgttg tttaatcgcc
    38881 ggattaacaa aaatgcgcgt gacgtctttg tcctgggcgg cgagtttgat caagctgaaa
    38941 atttccggct tccacagcgt ggggacaacg cgtttaccgt cgcgggatac taagtccagt
    39001 gcttgcgggc gcaagagctg cgcggaggtc cagcgagttt tcggcagttg cagaaagata
    39061 tcgacatcca gtccggtctg gtggctggca tgaccgccgt tgaaacgccc accagcgggc
    39121 atccccatat cgccaatcag caccgtaccc atgcccagat tgctcacctg gctactcaga
    39181 cgctggataa acatcaccag gtccgggtga ccgaaatagc gacgctgatc ggtacgcatg
    39241 acctgataat gttcggactg tatcggcagc gtgtcagcgc cgacgataca gccattagaa
    39301 aaactgccta tcgattgtgc gctacccggc acaggttggg ttattttttg ccacggcgtc
    39361 gctgccaggc tgacgctact ggcaagcagg gccagtagcg caatcgcggt tttgttcatt
    39421 ttttaccagc gtggaatatc agtcttcaca tcggcatttt gcgcccgctg ccgtaacagg
    39481 tgatccatta atacgatcgc cagcatcgct tctgcgatcg gcacggcgcg gatcccgaca
    39541 cagggatcgt gacggccttt ggtgatcatc tcaacttctt cgccaaagcg gttaatggtg
    39601 cgccccggca cggtaatgct ggaggtcggt ttcagcgcca tatgggcaat gatttgctgc
    39661 ccgctgctga taccgccaag aatgccgccc gcatggttgc tctggaaacc gtctttggtg
    39721 atttcgtcgc gattctgact gccgcgcagc gccactacgt caaaaccgtc gccaatttcc
    39781 acgcctttca ccgcgttgat gctcatcagc gcatgggcga tgtcggcatc caggcggtca
    39841 aagaccggct caccaagccc ggcaggaacg ccactggcaa caacggtgac tttcgcgcca
    39901 atagagtcgc cctctttttt cagcgcgcgc atcagttcat ctaacgcgtc gattttgtcc
    39961 gggtccgggc agaagaacgg attttgttca acctgcgacc agtctttgat ttccagcgga
    40021 atgtcgccca tctgggtcag gcagccgcgg atttcaatac caaatttctc ggcgagatat
    40081 tttttggcaa tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga agaacgtccg
    40141 ccgccgcgat aatcacgcag accgtatttt tgttcgtagg tgtaatcagc atggcctggg
    40201 cggaaaacgt ctttaattgc gctgtaatcc tgagaacgct gatcggtatt ttcgatcaac
    40261 aaaccaatgc tggtgccggt agtaacacct tcaaaaacac cggagagaat tttgacctga
    40321 tccggctcac gacgctgggt ggtatagcgc gatgtcccag ggcgacgacg atcaaggtca
    40381 tgttgcaggt ccgcttccgt cagcggaatg cctggcggaa caccatcgac gatgcagccg
    40441 agcgccagcc cgtgcgattc gccgaaggtg gttacgcgaa agagttgtcc aattgtgttt
    40501 ccagccatca cggctccgtt attgttgtgt ttgcgtgttt acttaatctt tataaatcgc
    40561 gaaatgttca cgggccgcca gcagctgctc tttggtgagc ataaacacac catcgccgcc
    40621 gttatcaaac tccagccagg tgaacggaac atccggatat tgttccataa gatgtaccat
    40681 gctgttgccg acttcacaaa tcagcacacc atcatcagca aggtaatctg ccgcgttacc
    40741 gagaatgcga cgcgtcagtt tcaggccgtc agtgccagat gccaggccca gttctggctc
    40801 gtgacggtat tcgtttggca ggtcggacat atcttccgca tcgacatacg gcgggttagt
    40861 gacaatcagg tcgtactgca ctttcggcaa atcacggaac agatcggaac gaatcggaat
    40921 gacgttgtgg atcagaccgt gttcttcgat gttctgttcg gcaaccgcca gcgcgtcggg
    40981 agagatatct accgcatcga cttccgcttc cgggaaggca taagcacagg caatggcaat
    41041 gcagccgcta ccggtacaca tatctaaaat atgctgcggt tgcttgctga taagtccggc
    41101 aaatttattg ttgatcaatt caccaatcgg cgagcgcggc accagcacgc gttcatcgac
    41161 gtaaaattca tgaccgcaga accaggcttt attggtcagg taagccaccg gaatgcgttc
    41221 gttgacgcgg cggatcacgc gctcaacaat acggtgtttt tcgctggagg tcagacgcgc
    41281 ggtgcgcata tcttccggaa tatccagcgg caggtagagc gacggcaaca ccaattgtac
    41341 cgcttcatcc cacgggttat cggtgccgtg accgtaccag atatttgccg cgctgaagcg
    41401 gctaaccgac cagcgcaaca tgtcctgaat ggtttgcagc tcattaactg cttcatcaac
    41461 gaaaatttta tccacgtatt cctccagggc atgatcgcaa taatttcggc ggctagtttg
    41521 ccatgaagat gacgataaat cagcattcac gcgcggtgag tgaggaaaaa tacgtttaaa
    41581 acgatcgatt gcgctacgag tcgggtaaac tgtaggaaaa ttagaaatag agacgcataa
    41641 atgaaaaaga aaacaacact tagcgaggag gaccaggctc tgtttcgcca gttgatggcg
    41701 gggactcgca agattaagca ggacaccatt gtccatcggc cacagcgtaa aaaagtcagc
    41761 gaagtgccgg tgaaacgctt gatccaggag caggctgatg ccagccatta tttctcagat
    41821 gagtttcagc cgttactgaa taccgaaggc ccagtgaaat atgtccgccc ggatgttagt
    41881 cattttgaag cgaagaagtt gcgacgcggt gattattcgc cggaattgtt tttggattta
    41941 cacggtctga cgcagctaca ggccaagcag gaactggggg cgttgattgc cgcctgccgc
    42001 cgtgaacatg tgttttgcgc ttgcgtaatg cacgggcacg ggaagcatat tttaaaacag
    42061 caaacgccgc tgtggctggc acaacatccg catgtaatgg catttcacca ggcgccgaaa
    42121 gagtacggcg gagatgcagc gttgttagtg ttgattgaag tggaagagtg gctaccgccg
    42181 gagttgccat gaatattagg gggcggatgc gttttgccat ttcgccctga atgctgggac
    42241 caaatgccgg atgcggggta aacgccttat ccggctacga acactgtcga ggctaataat
    42301 attaattaaa gttcattaag aatatattga aagcgacatg tagcatcaaa actttgccgt
    42361 tcactagtat caacacgcac ctgcatgtca tggagttgtt tatcagttac aggatgtcgg
    42421 tttgccgtta cgttcacttt attgttatca caaccctgga cactaaacgt cattaattgt
    42481 gataccaggc gacaataggc tccacggtca tcactgaagt cactgccatc acataatcgc
    42541 ccatctttca ttaagaatgt cggcaacgat ttctcaataa ccccccccgt agattgcagg
    42601 cttaatgatt tattatgaac aacggtgttt tcatcccagg aaatatcata gcttgctact
    42661 ggctctgttg gacaatttga ttggtgcgca ttcggcccct gataataata tggccccgcc
    42721 tgcggaggcc ttgctgtgct ataaattgat gttgtcacca cccaagggca accatgggtt
    42781 gtacccgtaa catgtacatt tacaggaagt ctatgacaaa cccaaccaga ggtcataccg
    42841 ggacaataag aatcatctac tatcggtgcc cgccctggcg gaattgttat gtctgggggg
    42901 acacccggtc cagtccaggt taagttaggc acaactcctg cctgtaccat aatcccccct
    42961 tgtgaatatg tcccataata tgccccttca ggggttaaca catctatttt ataaacgata
    43021 gatgttgtta gcccactgcc ataagatgcg ccataagtct gaacattatt aaatgtaacg
    43081 tctgcttgta cagaagaatt aagcccgaac aataacatac caaataaaat cacttttatt
    43141 ttcatttaga tatactcaaa aattgccgtc aggctggcag taaattcacc agaagtgact
    43201 tcacgcccag atttcgcctg caaccaagcc ttaaagttaa gattattagc accgttactc
    43261 aggttaaata ttgcccctgc cgaattgttg atggggacct gtatcccatc aactgtttcc
    43321 atgccaatgc tcacaccttg cgcacccgat gatgcatcca aagccagaaa ccctggcaaa
    43381 tcgctatctt cggtaccggt taacgtaact ctaactttgt aaccaacagg ccctttgcaa
    43441 tctttcaatt gaaaaacgac cggcacacga tcagattgtt cccgtgacat taaatctctg
    43501 ctgctgatac ttgggaaatg gacttcagcc agcacttgtc cttgtactac cagggtacaa
    43561 gatttactta ataaatttcc gtagaagtgt aaattattgt ctgcacgcgc tgtgctattt
    43621 cctgccagta acagcattac tatgagtgcg aaccaggttt tcatcttcat tgatattcca
    43681 cccgtaatgt ggccgaagct gaaaacgttc cgtctgttaa tacagcatct accgccttca
    43741 cggggacagc ctctaattgt ggtttcgtat cggcgttaat ttgtatcggt gtatttactc
    43801 taaatggctg accattttgc cgaagtttaa tccctaatcc tgttacatca gtttcgattg
    43861 cagcatcatc ataacttgtt tgtgtgccta tccatgtcaa tgtcatatcc caggaactac
    43921 cgggtgtaac agcatcacat ttaatatcgt agggcacgac ctctcgccca ttatttccat
    43981 caatgctatc gataattaca tccctgaact ccacctcgat agtgtttcca tcactaatag
    44041 tacatgccgg aggcaataca agagttcctt tgaaggtgat atccggtgat gcattcacaa
    44101 taccgctgct catacagagc aataaataca agagtatttt cattgataat ccaccaccat
    44161 tgttaaactc ccactaaaat cggcccctga aagttgtgca ccactttgtt ttaccgggac
    44221 tgcattaagt ttcggctgtg atgtgatatt gaaaggggtc cagttgtttt ctccaatgga
    44281 gaatagtgag ttgttatctg cattctcgat tcgaatacca aatcctggaa cccctacatc
    44341 aacgactttt tctccattaa ttgtggtggt gtttcctctc aactgcatac gtaaatcatc
    44401 agcttttcgg tcagggcatg acaacgtata tcctgcatct ttacgataat tactaccatt
    44461 gatatcgttg atattcacag tctgaaaatc tactgaactc cccgttacgc tacatggcgg
    44521 aggaggatct acatgtacct gtaaaatgag atcaacaggt tttgaatttg caaggataat
    44581 gttatatatc ccactaacgc ccagcagtaa tatacccacc cccacaacaa aaatttgtct
    44641 catgtcgctc tcccggcatc agcctttgcg gctctgctct acatccacct ggcaggtatc
    44701 accgccacat ttaaaataca gcggcatccg tgcgccatag tcgttgacgt aagacagcac
    44761 cgggacgcta tccattttga cttttaacgg cacagaggtt tttggcgata tcaccatggg
    44821 ggaaaatccc gctgccggat tgccattttt ctgcgtgctg gcgttactga taaccacgta
    44881 gtacggcgta ggattattga cggtaaactc ctgaccgctg cgggttaagg tcaccttatg
    44941 ctgccacggg tttttcatgc tgacgttttc cagcgccttt ggtcgccaga acagcttgat
    45001 gcgcgtttgc agcgctatct gtaacgtgtt gggtttattc gatttcggcg gaatttcacg
    45061 cacgttgaag tagaacacgc tctcgcggtc ggcaggcagc ttgttgatat ccggcatccc
    45121 ctgcaccttc acctggccgt tcatcatgga atcgatgcgc tgcaccggcg gcaggaccgt
    45181 cagtggcgag ctaatcctgt taccctgttc atcttccatc cagctttgtg ccagatacgg
    45241 cattttcggg tcgttattac gcagcgtgac gctgattgat ttatcactct cattaaaaat
    45301 caggcgtgta cggtctggcg tgacagaggc ctgtgctgac aggctggcag tggttagtag
    45361 caatgccagc aacattgtgg attttttggc agagcaaaat agattcgtca tggatcattc
    45421 cttaatcacg ttattaattg gcggataccg ataccggtgc ttccgttggc atcacctgct
    45481 gggtctgctc ctggatcatc ggcttaattt catgaggcat cgaaggagat atcgcccctg
    45541 tttgctggca tggtaataac aggccattga acagatcggc tggcagcggg tcaggcagat
    45601 gaatatcgca gtgggccaca ccgccccatg aaacgatcat atgctcgcca ggtttgacgc
    45661 ccgcgaggta gacgttgccg tcatcgtcaa ccagaccgac gttctgcgcg ctgtcgtttt
    45721 tcacttccgc gccaaacggc ggataactgc catcttgcag acgcagcacc gccatcgctt
    45781 tttgcccgct gatcaccgaa aacttacggt agccaatggc tccttcggta agcgtcgcct
    45841 gcaccacgga ctgggtggct tcggcgtttt ccggcaggtt gttaaggtcg atatacgcct
    45901 ggttgcggta gtagttgttg acgtcggcca ccacggcctt accgaacata ttggtgtaaa
    45961 ccgccgcacc gtttccttcc accggaacac cggccacgcc atcggcatca atcagcaggc
    46021 gcgtaccgcc catattctgg gtacggtgga gcgcgccacc ctgcgcggtc agcgtcgcgc
    46081 cgccctgtaa ggaaatacct gccgaggtgt actgtccttc atgatagttg gcgctgagat
    46141 cgacctgcgc cagcgatccg tcgtggctgt aataaccgtc aacgctggag tgattgtcgc
    46201 tggtgcctac gttcaactgg taatgggttg cgtcatcgac acggctgaaa taccccacct
    46261 ggctgctgtc cgaaccgctg ccgtagttgc cgttatagct gatggtgctg ctgtcacccc
    46321 acggcatgct gagcgatatg taaacacctt tatcggactg gttgtcatac tcgtagcggt
    46381 atccggtcat ggaaatgctc atgttgcgga tactgcccag gttgaagtag tgcgagagca
    46441 tgatgttgta gttagtctgt tcgtcgcgat cccagtaggt atggcgggtg tagttgagat
    46501 aaacagatac accggcatcc ctgaagttct ggttgtaggt ggcggtgtac atctctttgt
    46561 cgttgccggt gcgcaccatt tcgctgtcgc tggcatcgag atactcgctc atggtcatga
    46621 agttctcttc cgagaagcgg tatccggcaa aagtgacgcg gctgttcagt tcatcgaagt
    46681 ctttggaata gctcacgcga aacgagttac cgtccagtga acctttaccg taggcagttt
    46741 ctttatccag gcgggtatgc gagtgggtga catcaaaggc caccgcacca aacacagaca
    46801 gatcgcgacc gacgccaagc gccgccgact gatagtgttc atctgccagc gccccgccgt
    46861 atagcgacca gccgttggca atcccccagg aagcttcgcc gccggagaag aaaccgcctt
    46921 ccacgtggtg cccccactcc tgcgggcggc ccatcatcag tttatagcgc acctggccgg
    46981 ggcgggtcag gaacggcatt gacgcagtgt taatgtcata ttcctgcacc tgaccgttct
    47041 gctcttcaat gcggatatgc agcgtaccgg agacggaatc gccaagatcc tggatacgga
    47101 acggccccgc cggaacctga gtttcgtaaa tcacgcggcc catctggctg acggtcactt
    47161 tcgcggtggt gtgcgccacg ccggagatat ccggcgcata gccgcgcaga ttcggcggca
    47221 acatttgatc gtcggtgctg atactgccac ccacgtagtt aaagccgtcg aaaatatcag
    47281 aattcaggta gtcttcacca aggccaagtt tggcttttag cgacggtaag gcccgccagg
    47341 cgtagtagcg gctccactcc cagttttttt gcgtgtcatc accgttgata acatcgtcat
    47401 cattgctttt actatgcaaa taatcagtct gccagtcggc acgaagacgc catgcgccaa
    47461 ggttcacccc aaccgtcccg ttaccgctaa tttcattact gtcatccccg ccattttctt
    47521 catgtcgtgt ctgggcggta atactgtagt cagcaattaa accagatata ccgtcatccc
    47581 agcgtgaagg cggatcccag ttgatgtcgg tatattcaag gtaagcctgg ggtaatgaaa
    47641 tgactaacgc tgactgactc aggtcagctt taatatcaat gccttccagt tgaccgggtt
    47701 tcaggcattt gccgtcgtgg atccattgca ggttttttgc cacatcctct tttaagccaa
    47761 actgcgcgac cagttcaggc gtcaggcagg cataggtttt actggcatcg ttctcagagg
    47821 cgtaccagta aatatcgtat tcttccgtca gcggctgttt atttagttga acctgtaaat
    47881 tgtatttccc aggttcgaca taaccctggc tggaaaatcg cttcagatcg atttttgtgt
    47941 cgccttttaa ctccagaaaa cgggaatcaa actgaatttc atcttcagcc caggcattga
    48001 cataactccc ggacattgcc aatgcaacgc agcagggaag tacccgcagt cggaaaagag
    48061 aatggtcagg catacacgta tccatccgta attattataa tttgcgcgaa ttccatttaa
    48121 aaaccccata gcccgaaagc tatgggggta tcacttagag ataagtaatc tggaaggtcg
    48181 tgttggcttc aaaattacct aaatctggcg catcagcagc gcccaccagc caggctttaa
    48241 agtccagcgt ctgcttcgct ttgcctttgt tggtcgccgt atcgtttacg attttctgtt
    48301 cgatacccgc gccgctttta taagaggttc cctgagcgtc accaatggcc aggctgacat
    48361 tgttaaatgc cgcaccagta tcggtattgt aaatggtgta gtaattgccg ctgttggtag
    48421 aagacgcgtt accggtgaag gtagtggtca tcgttgtctg ggtgtcaaac acacagtctt
    48481 gcagatggat ctggaaagct ttcggtgtag aagtcccgtt gtttttcagg atccctgaac
    48541 caatgtcacc catatcaact tccagtttgt gatcatcagg aacgatggag cacggggaat
    48601 cttcaatggt gccgtagaaa cgtgctgtac cattgttgcc agcggcgatt gttgaagtag
    48661 aagcgaacgc acccattacc attgttgcag caatagctgt ttttacaaac ttactcatta
    48721 tttgtcctta aaccataaat aattaaaaat ataaacattg caatacattg attcagtcaa
    48781 tagcaattat ttatacccag ccattgaata agctatccga tctaactaac aagaaaatag
    48841 gtcgcataag aagatattct ttggtgtggg cggatggtga tattttgtct ttattgtttc
    48901 aaattatatt tacttaacag
//
LOCUS       DAZKFU020000006        48186 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZKFU020000006 DAZKFU020000000
VERSION     DAZKFU020000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 48186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.006"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            331..1647
                     /gene="fucP"
                     /locus_tag="ACHOQQ_RS01345"
                     /old_locus_tag="pgaptmp_000269"
     CDS             331..1647
                     /gene="fucP"
                     /locus_tag="ACHOQQ_RS01345"
                     /old_locus_tag="pgaptmp_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGU4468577.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1680..3455
                     /gene="fucI"
                     /locus_tag="ACHOQQ_RS01350"
                     /old_locus_tag="pgaptmp_000270"
     CDS             1680..3455
                     /gene="fucI"
                     /locus_tag="ACHOQQ_RS01350"
                     /old_locus_tag="pgaptmp_000270"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGU4468578.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3564..4982
                     /gene="fucK"
                     /locus_tag="ACHOQQ_RS01355"
                     /old_locus_tag="pgaptmp_000271"
     CDS             3564..4982
                     /gene="fucK"
                     /locus_tag="ACHOQQ_RS01355"
                     /old_locus_tag="pgaptmp_000271"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU4468579.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGIGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4984..5406
                     /gene="fucU"
                     /locus_tag="ACHOQQ_RS01360"
                     /old_locus_tag="pgaptmp_000272"
     CDS             4984..5406
                     /gene="fucU"
                     /locus_tag="ACHOQQ_RS01360"
                     /old_locus_tag="pgaptmp_000272"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGU4468580.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5464..6195
                     /gene="fucR"
                     /locus_tag="ACHOQQ_RS01365"
                     /old_locus_tag="pgaptmp_000273"
     CDS             5464..6195
                     /gene="fucR"
                     /locus_tag="ACHOQQ_RS01365"
                     /old_locus_tag="pgaptmp_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGU4468581.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(6239..7339)
                     /gene="rlmM"
                     /locus_tag="ACHOQQ_RS01370"
                     /old_locus_tag="pgaptmp_000274"
     CDS             complement(6239..7339)
                     /gene="rlmM"
                     /locus_tag="ACHOQQ_RS01370"
                     /old_locus_tag="pgaptmp_000274"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGU4468582.1"
                     /translation="MNKVVLLCRPGFEKECAAEISDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7332..7727)
                     /gene="ygdD"
                     /locus_tag="ACHOQQ_RS01375"
                     /old_locus_tag="pgaptmp_000275"
     CDS             complement(7332..7727)
                     /gene="ygdD"
                     /locus_tag="ACHOQQ_RS01375"
                     /old_locus_tag="pgaptmp_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGU4468583.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7746..8663)
                     /gene="gcvA"
                     /locus_tag="ACHOQQ_RS01380"
                     /old_locus_tag="pgaptmp_000276"
     CDS             complement(7746..8663)
                     /gene="gcvA"
                     /locus_tag="ACHOQQ_RS01380"
                     /old_locus_tag="pgaptmp_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGU4468584.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(9014..9241)
                     /gene="ygdI"
                     /locus_tag="ACHOQQ_RS01385"
                     /old_locus_tag="pgaptmp_000277"
     CDS             complement(9014..9241)
                     /gene="ygdI"
                     /locus_tag="ACHOQQ_RS01385"
                     /old_locus_tag="pgaptmp_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGU4468585.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9433..10638
                     /gene="csdA"
                     /locus_tag="ACHOQQ_RS01390"
                     /old_locus_tag="pgaptmp_000278"
     CDS             9433..10638
                     /gene="csdA"
                     /locus_tag="ACHOQQ_RS01390"
                     /old_locus_tag="pgaptmp_000278"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGU4468586.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRTGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10638..11081
                     /gene="csdE"
                     /locus_tag="ACHOQQ_RS01395"
                     /old_locus_tag="pgaptmp_000279"
     CDS             10638..11081
                     /gene="csdE"
                     /locus_tag="ACHOQQ_RS01395"
                     /old_locus_tag="pgaptmp_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGU4468587.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRKTFAPLTQWEEKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAEHGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(11132..11938)
                     /gene="tcdA"
                     /locus_tag="ACHOQQ_RS01400"
                     /old_locus_tag="pgaptmp_000280"
     CDS             complement(11132..11938)
                     /gene="tcdA"
                     /locus_tag="ACHOQQ_RS01400"
                     /old_locus_tag="pgaptmp_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGU4468588.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12176..13273)
                     /gene="mltA"
                     /locus_tag="ACHOQQ_RS01405"
                     /old_locus_tag="pgaptmp_000281"
     CDS             complement(12176..13273)
                     /gene="mltA"
                     /locus_tag="ACHOQQ_RS01405"
                     /old_locus_tag="pgaptmp_000281"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGU4468589.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13482..13558
                     /locus_tag="ACHOQQ_RS01410"
                     /old_locus_tag="pgaptmp_000282"
     tRNA            13482..13558
                     /locus_tag="ACHOQQ_RS01410"
                     /old_locus_tag="pgaptmp_000282"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13516..13518,aa:Met,seq:cat)
     gene            complement(13632..14885)
                     /gene="amiC"
                     /locus_tag="ACHOQQ_RS01415"
                     /old_locus_tag="pgaptmp_000283"
     CDS             complement(13632..14885)
                     /gene="amiC"
                     /locus_tag="ACHOQQ_RS01415"
                     /old_locus_tag="pgaptmp_000283"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGU4468590.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15117..16448
                     /gene="argA"
                     /locus_tag="ACHOQQ_RS01420"
                     /old_locus_tag="pgaptmp_000284"
     CDS             15117..16448
                     /gene="argA"
                     /locus_tag="ACHOQQ_RS01420"
                     /old_locus_tag="pgaptmp_000284"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGU4468591.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16510..18336)
                     /gene="recD"
                     /locus_tag="ACHOQQ_RS01425"
                     /old_locus_tag="pgaptmp_000285"
     CDS             complement(16510..18336)
                     /gene="recD"
                     /locus_tag="ACHOQQ_RS01425"
                     /old_locus_tag="pgaptmp_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGU4468592.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18336..21878)
                     /gene="recB"
                     /locus_tag="ACHOQQ_RS01430"
                     /old_locus_tag="pgaptmp_000286"
     CDS             complement(18336..21878)
                     /gene="recB"
                     /locus_tag="ACHOQQ_RS01430"
                     /old_locus_tag="pgaptmp_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGU4468593.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESRLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLSDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYSLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAELITLMDEMFAGMTLEEA"
     gene            complement(21871..24759)
                     /gene="ptrA"
                     /locus_tag="ACHOQQ_RS01435"
                     /old_locus_tag="pgaptmp_000287"
     CDS             complement(21871..24759)
                     /gene="ptrA"
                     /locus_tag="ACHOQQ_RS01435"
                     /old_locus_tag="pgaptmp_000287"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGU4468594.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAASIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24935..28303)
                     /gene="recC"
                     /locus_tag="ACHOQQ_RS01440"
                     /old_locus_tag="pgaptmp_000288"
     CDS             complement(24935..28303)
                     /gene="recC"
                     /locus_tag="ACHOQQ_RS01440"
                     /old_locus_tag="pgaptmp_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGU4468595.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGAVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(28316..28639)
                     /gene="ppdC"
                     /locus_tag="ACHOQQ_RS01445"
                     /old_locus_tag="pgaptmp_000289"
     CDS             complement(28316..28639)
                     /gene="ppdC"
                     /locus_tag="ACHOQQ_RS01445"
                     /old_locus_tag="pgaptmp_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGU4468596.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(28624..29031)
                     /gene="ygdB"
                     /locus_tag="ACHOQQ_RS01450"
                     /old_locus_tag="pgaptmp_000290"
     CDS             complement(28624..29031)
                     /gene="ygdB"
                     /locus_tag="ACHOQQ_RS01450"
                     /old_locus_tag="pgaptmp_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGU4468597.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMYSWQTQPAVQCLQYAGTDARVCLRLLADNEALLIAGYEGVSL
                     WRAGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(29028..29591)
                     /gene="ppdB"
                     /locus_tag="ACHOQQ_RS01455"
                     /old_locus_tag="pgaptmp_000291"
     CDS             complement(29028..29591)
                     /gene="ppdB"
                     /locus_tag="ACHOQQ_RS01455"
                     /old_locus_tag="pgaptmp_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4468598.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(29582..30052)
                     /gene="ppdA"
                     /locus_tag="ACHOQQ_RS01460"
                     /old_locus_tag="pgaptmp_000292"
     CDS             complement(29582..30052)
                     /gene="ppdA"
                     /locus_tag="ACHOQQ_RS01460"
                     /old_locus_tag="pgaptmp_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4468599.1"
                     /translation="MKTQRGYTLIETLVAMLILVTLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30237..31031)
                     /gene="thyA"
                     /locus_tag="ACHOQQ_RS01465"
                     /old_locus_tag="pgaptmp_000293"
     CDS             complement(30237..31031)
                     /gene="thyA"
                     /locus_tag="ACHOQQ_RS01465"
                     /old_locus_tag="pgaptmp_000293"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGU4468600.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(31038..31913)
                     /gene="lgt"
                     /locus_tag="ACHOQQ_RS01470"
                     /old_locus_tag="pgaptmp_000294"
     CDS             complement(31038..31913)
                     /gene="lgt"
                     /locus_tag="ACHOQQ_RS01470"
                     /old_locus_tag="pgaptmp_000294"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGU4468601.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(32064..34310)
                     /gene="ptsP"
                     /locus_tag="ACHOQQ_RS01475"
                     /old_locus_tag="pgaptmp_000295"
     CDS             complement(32064..34310)
                     /gene="ptsP"
                     /locus_tag="ACHOQQ_RS01475"
                     /old_locus_tag="pgaptmp_000295"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4468602.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPCFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34323..34853)
                     /gene="rppH"
                     /locus_tag="ACHOQQ_RS01480"
                     /old_locus_tag="pgaptmp_000296"
     CDS             complement(34323..34853)
                     /gene="rppH"
                     /locus_tag="ACHOQQ_RS01480"
                     /old_locus_tag="pgaptmp_000296"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGU4468603.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(35206..35352)
                     /gene="ygdT"
                     /locus_tag="ACHOQQ_RS01485"
                     /old_locus_tag="pgaptmp_000297"
     CDS             complement(35206..35352)
                     /gene="ygdT"
                     /locus_tag="ACHOQQ_RS01485"
                     /old_locus_tag="pgaptmp_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGU4468604.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFGGSVLNLVAHSVDK
                     YGIG"
     gene            35538..36227
                     /gene="mutH"
                     /locus_tag="ACHOQQ_RS01490"
                     /old_locus_tag="pgaptmp_000298"
     CDS             35538..36227
                     /gene="mutH"
                     /locus_tag="ACHOQQ_RS01490"
                     /old_locus_tag="pgaptmp_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGU4468605.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            36296..37009
                     /gene="ygdQ"
                     /locus_tag="ACHOQQ_RS01495"
                     /old_locus_tag="pgaptmp_000299"
     CDS             36296..37009
                     /gene="ygdQ"
                     /locus_tag="ACHOQQ_RS01495"
                     /old_locus_tag="pgaptmp_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4468606.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            37147..37365
                     /gene="ygdR"
                     /locus_tag="ACHOQQ_RS01500"
                     /old_locus_tag="pgaptmp_000300"
     CDS             37147..37365
                     /gene="ygdR"
                     /locus_tag="ACHOQQ_RS01500"
                     /old_locus_tag="pgaptmp_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGU4468607.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            37473..38513
                     /gene="tas"
                     /locus_tag="ACHOQQ_RS01505"
                     /old_locus_tag="pgaptmp_000301"
     CDS             37473..38513
                     /gene="tas"
                     /locus_tag="ACHOQQ_RS01505"
                     /old_locus_tag="pgaptmp_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGU4468608.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(38545..39738)
                     /gene="lplT"
                     /locus_tag="ACHOQQ_RS01510"
                     /old_locus_tag="pgaptmp_000302"
     CDS             complement(38545..39738)
                     /gene="lplT"
                     /locus_tag="ACHOQQ_RS01510"
                     /old_locus_tag="pgaptmp_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGU4468609.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(39731..41890)
                     /gene="aas"
                     /locus_tag="ACHOQQ_RS01515"
                     /old_locus_tag="pgaptmp_000303"
     CDS             complement(39731..41890)
                     /gene="aas"
                     /locus_tag="ACHOQQ_RS01515"
                     /old_locus_tag="pgaptmp_000303"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGU4468610.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            42476..43507
                     /gene="galR"
                     /locus_tag="ACHOQQ_RS01520"
                     /old_locus_tag="pgaptmp_000304"
     CDS             42476..43507
                     /gene="galR"
                     /locus_tag="ACHOQQ_RS01520"
                     /old_locus_tag="pgaptmp_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGU4468611.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(43514..44776)
                     /gene="lysA"
                     /locus_tag="ACHOQQ_RS01525"
                     /old_locus_tag="pgaptmp_000305"
     CDS             complement(43514..44776)
                     /gene="lysA"
                     /locus_tag="ACHOQQ_RS01525"
                     /old_locus_tag="pgaptmp_000305"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGU4468612.1"
                     /translation="MPHSLFSTDTALTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            44898..45833
                     /gene="lysR"
                     /locus_tag="ACHOQQ_RS01530"
                     /old_locus_tag="pgaptmp_000306"
     CDS             44898..45833
                     /gene="lysR"
                     /locus_tag="ACHOQQ_RS01530"
                     /old_locus_tag="pgaptmp_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGU4468613.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(45820..46512)
                     /gene="ygeA"
                     /locus_tag="ACHOQQ_RS01535"
                     /old_locus_tag="pgaptmp_000307"
     CDS             complement(45820..46512)
                     /gene="ygeA"
                     /locus_tag="ACHOQQ_RS01535"
                     /old_locus_tag="pgaptmp_000307"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGU4468614.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEELSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(46641..48059)
                     /gene="araE"
                     /locus_tag="ACHOQQ_RS01540"
                     /old_locus_tag="pgaptmp_000308"
     CDS             complement(46641..48059)
                     /gene="araE"
                     /locus_tag="ACHOQQ_RS01540"
                     /old_locus_tag="pgaptmp_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGU4468615.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        11814 a        13075 c        11934 g        11363 t
ORIGIN      
        1 aacggtcatt tttctattca cctaagccag gaatgcccgt tttcggcata aacaattaat
       61 acggtcatct ggtttgtgtt ttttatgatt tattttctga aacgggcatg aaatttcgat
      121 tattaaagtg atggtagtca cataaagtca ccttccagct aataagtgtg accgccgtca
      181 tattacagag cgttttttat ttgaaaatga atccatgagt tcatttcaga caggcaaata
      241 ttcactgata tgaagcccga actcgctggt tttgcacttt tgaaaacata accgattacg
      301 tgcttaagct tctgaaccta agaggatgct atgggaaaca catcaataca aacgcagagt
      361 taccgtgcgg tagataaaga tgcagggcaa agcagaagtt acattattcc attcgcgctg
      421 ctgtgctcac tgttttttct ttgggcggta gccaataacc ttaacgacat tttattacct
      481 caattccagc aggcttttac gctgacaaat ttccaggcag gcctgatcca atcggccttt
      541 tactttggtt atttcattat cccaattcct gctgggatat tgatgaaaaa actcagttat
      601 aaagcaggga ttattaccgg gttattttta tatgccttgg gtgctgcatt attctggccc
      661 gcggcagaaa taatgaacta caccttattt ttagttggcc tatttattat tgcagccgga
      721 ttaggttgtc tggaaactgc cgcaaaccct tttgttacgg tattagggcc ggaaagtagt
      781 ggtcacttcc gcttaaatct tgcgcaaaca tttaactcgt ttggcgcaat tatcgcggtt
      841 gtctttgggc aaagtcttat tttgtctaac gtgccacatc aatcgcaaga cgttctcgat
      901 aaaatgtctc cagagcaatt gagtgcgtat aaacacagcc tggtattatc ggtacagaca
      961 ccttatatga tcatcgtggc tatcgtgtta ctggtcgccc tgctgatcat gctgacgaaa
     1021 ttcccggcat tgcagagtga taatcacagt gacgccaaac aaggatcgtt ctccgcatcg
     1081 ctttctcgcc tggcgcgtat tcgccactgg cgctgggcag tattagcgca attctgctac
     1141 gttggcgcac aaacggcctg ctggagctat ttgattcgct acgctgtaga agaaattcca
     1201 ggtatgactg caggctttgc cgctaactat ttaaccggaa ccatggtgtg cttctttatt
     1261 ggtcgtttca ccggtacctg gctcatcagt cgcttcgcac cacacaaagt cctggccgcc
     1321 tacgcattaa tcgctatggc actgtgcctg atctcagcct tcgctggcgg tcatgtgggc
     1381 ttaatagccc tgactttatg cagcgccttt atgtcgattc agtacccaac aatcttctcg
     1441 ctgggcatta agaatctcgg ccaggacacc aaatacggtt cgtccttcat cgttatgacc
     1501 atcattggcg gcggtattgt cactccggtc atgggttttg tcagtgacgc ggcgggcaac
     1561 atccccactg ctgaactgat ccccgcactc tgcttcgcgg tcatctttat ctttgcccgt
     1621 ttccgttctc aaacggcaac taactgaaca tattttccga ataaagtgag gaatctgtaa
     1681 tgaaaaaaat cagcttaccg aaaattggta tccgcccggt tattgacggt cgtcgcatgg
     1741 gtgttcgtga gtcgcttgaa gaacaaacaa tgaatatggc gaaagctacg gccgcactgc
     1801 tgaccgagaa actgcgccat gcctgcggag ctgccgtcga gtgtgtcatt tccgatacct
     1861 gtatcgcggg tatggctgaa gccgctgctt gcgaagaaaa attcagcagt cagaatgtag
     1921 gcctcaccat tacggtaacg ccttgctggt gctatggcag tgaaaccatc gacatggatc
     1981 caacccgccc gaaagccatt tggggcttta acggcactga acgtcccggc gctgtttacc
     2041 tggcagcggc tctggcagct cacagccaga aaggcatccc ggcattctcc atttacggtc
     2101 atgacgttca ggatgccgat gacacatcga ttcctgccga tgttgaagaa aaactgctgc
     2161 gctttgcccg cgccggtttg gccgtcgcca gcatgaaagg taaaagctat ctgtcgctgg
     2221 gcggcgtttc gatgggtatc gccggttcta ttgttgatca caacttcttt gaatcctggc
     2281 tgggaatgaa agtccaggcg gtggatatga ccgaactgcg tcgccgtatc gatcagaaga
     2341 tttacgacga agccgaactg gaaatggcac tggcctgggc tgataaaaac ttccgctatg
     2401 gcgaagatga aaataacaaa cagtatcaac gtaatgccga gcaaagccgc gcagttctgc
     2461 gcgaaagttt actgatggcg atgtgtatcc gcgacatgat gcaaggcaac agcaaactgg
     2521 ccgatattgg tcgcgtggaa gaatcacttg gctacaacgc catcgctgcg ggcttccagg
     2581 ggcaacgtca ctggaccgat caatatccca atggtgacac cgccgaagcg atcctcaaca
     2641 gttcatttga ctggaatggc gtgcgcgaac cctttgtcgt ggcgaccgaa aacgacagtc
     2701 ttaacggcgt ggcaatgcta atgggtcacc agctcaccgg caccgctcag gtatttgccg
     2761 atgtgcgtac ctactggtca ccagaagcaa ttgagcgtgt aacggggcat aaactcgatg
     2821 gactggcaga acacggcatc atccatttga tcaactcagg ttctgctgcg ctggacggtt
     2881 cctgtaaaca acgcgacagc gaaggtaacc cgacgatgaa gccgcactgg gaaatctctc
     2941 agcaagaggc tgacgcttgc ctcgccgcta ccgaatggtg cccggcgatc cacgaatact
     3001 tccgtggcgg cggttactct tcccgcttcc ttaccgaagg cggcgtcccg ttcaccatga
     3061 ctcgtgtcaa catcatcaaa ggcctgggac cggtactgca aatcgcggaa ggctggagcg
     3121 tggaattgcc gaaggatgtg catgacatcc tcaacaaacg caccaactca acctggccaa
     3181 ccacctggtt tgcaccgcgc ctcaccggta aagggccgtt tacggatgtg tactcggtaa
     3241 tggcgaactg gggcgctaac catggggttc tgaccatcgg ccacgttggc gcagacttta
     3301 tcactctcgc ctccatgctg cgtatcccgg tatgtatgca caacgttgaa gagaccaaag
     3361 tgtatcgtcc ttctgcctgg gctgcgcacg gcatggatat tgaaggccag gattaccgcg
     3421 cttgccagaa ctacggtccg ttgtacaagc gttaatacat tactccctga tgtgatgccc
     3481 ggtcgctccg gctaccgggc ctgaacaagc aagagtggtt agccggataa gcaatgttat
     3541 ccggctatat tgcaggagcg attatgaaac aagaagttat cctggtactc gactgtggcg
     3601 cgaccaatgt cagggccatc gcggttaatc ggcagggaaa aattgttgcc cgcgcctcaa
     3661 cgcctaatgc cagcgatatc gcgatggaaa acaacacctg gcaccagtgg tctttagacg
     3721 ccattttgca acgctttgct gattgctgtc ggcaaatcaa tagtgaactg actgaatgcc
     3781 acatccgcgg tatcgccgtc accacctttg gtgtggatgg cgctctggta gataagcaag
     3841 gcaatctgct ctatccgatc attagctgga aatgtccgcg aacagcagcg gttatggaca
     3901 atattgaacg gttaatctcc gcacagcggt tgcaggctat ttctggcgtc ggagccttta
     3961 gtttcaatac gttatataag ttggtgtggt tgaaagaaaa tcatccacaa ctgctggaac
     4021 gcgcgcacgc ctggctcttt atttcgtcgc tgattaacca ccgtttaacc ggcgaattca
     4081 ctactgatat cacgatggcc ggaaccagcc agatgctgga tatccagcaa cgcgatttca
     4141 gtccacaaat tttacaagcc accggtattc cacgccgact cttccctcgt ctggtggaag
     4201 cgggtgaaca gattggtacg ctacagaaca gcgccgcagc aatgctcggc ttacccgttg
     4261 gcataccggt gatttccgca ggtcacgata cccagttcgc cctttttggc gctggtgctg
     4321 aacaaaatga acccgtgctc tcttccggta catgggaaat tttaatggtt cgcagcgccc
     4381 aggttgatac ttcgctgtta agtcagtacg ccggttccac ctgcgaactg gatagccagg
     4441 cagggttgta taacccaggt atgcaatggc tggcatccgg cgtgctggaa tgggtgagaa
     4501 aactgttctg gacggctgaa acaccctggc aaatgttgat tgaagaggct cgtctgatcg
     4561 cgcctggcgc ggatggcgtg aaaatgcagt gtgatttatt gtcgtgtcag aacgctggct
     4621 ggcaaggagt gacgcttaat accacgcggg ggcatttcta tcgcgcggcg ctggaagggt
     4681 taactgcgca attacagcgc aatctacaga tgctggaaaa aatcgggcac tttaaagcct
     4741 ctgaattatt gttagtcggt ggaggaagtc gcaacacatt gtggaatcag attaaagcca
     4801 atatgcttga tattccggta aaagttctcg acgacgccga aacgaccgtc gcaggagctg
     4861 cgctgttcgg ttggtatggc ataggggaat ttaacagccc ggaagaagcc cgcgcgcaga
     4921 ttcattatca gtaccgttat ttctacccgc aaactgaacc tgaatttata gaggaagtgt
     4981 gaaatgctga aaacaatttc gccgttaatt tctcccgaac tattgaaagt gctggcagag
     5041 atgggacatg gagatgaaat tattttttcc gatgctcact ttcccgccca ttcgatggga
     5101 ccgcaggtga tccgcgctga tggcctgttg gtgagcgact tgctccaggc gattatcccg
     5161 ttatttgaac tggacagtta tgcaccgccg ctggtgatga tggcagcggt agaaggtgac
     5221 actctcgatc ctgaagtaga acgacgttac cgtaatgcgc tttcactaca agccccgtgt
     5281 cctgacatca tccgcatcaa tcgttttgcg ttttatgaac gggcgcaaaa agcctttgcg
     5341 atcgttatca caggcgaacg agcgaagtac gggaatattc ttttaaaaaa aggggtaaca
     5401 ccgtaatctc ataccggtac gcccgcatga cgcgggcggt tatcgaatga tggggtgaaa
     5461 aatatgaaag cggcacgcca gcaagcgata gtcgacctgc tgctgaacca taccagcctg
     5521 accacggaag ctctctctga acagctaaag gtcagtaaag aaaccattcg tcgcgatctc
     5581 aatgaattac agacgcaggg taaaattctg cgcaatcatg gacgcgctaa atatatccac
     5641 cgtcaaaatc aagacagtgg cgatcccttt cacatcaggc tgaaaagcca ttatgcgcat
     5701 aaagcagata tcgcgcgcga ggcgctcgcg tggattgaag aagggatggt gatagcctta
     5761 gacgccagtt caacctgctg gtatctggca cgccagttgc ctgacatcaa cattcaggtc
     5821 ttcaccaata gccatccgat ttgccatgaa ctcggtaaac gcgaacgcat tcaactgatc
     5881 agttccggcg gcacacttga gcgcaaatat ggctgttacg tcaatccctc gctgatttcc
     5941 caacttaaat cgctggaaat cgatctgttt attttttctt gtgaagggat cgatagcagc
     6001 ggcgcactgt gggactccaa tgccatcaac gctgattaca aatcgatgct attaaaacgt
     6061 gccgcgcaat cgttgttatt gattgataaa agtaaattta atcgttcagg ggaagcccgc
     6121 atcgggcatc tggatgaggt aacgcacatt atttctgatg agcgccaggt tgcaacttct
     6181 ttggtaacag cctgacggaa agggtagcag gccggagacg acgccccggc cttgcctgtt
     6241 atcgctcgtc gcgacgacca cccaccgcag cccagatgcg gcggacgtgc accgtcactt
     6301 cttcgcgatc gtgatacaac tgccgcgcct gaatctgagc atttatgcca tgttcatcaa
     6361 gctgtgcctg aatatacgcc agattgtgtg acacttcttc gtagcgtttt ttcatcggca
     6421 gtttgaggtt gaaaatggtt tcacggcacc agccattaac cagccactgc gccatcaatg
     6481 ccgcaacttt cgccggtttt tcaaccatat cgcataccat ccaggagata ttgctgcgtg
     6541 tcggacggaa tttgaacccg tcttcccgta gccacgtcac ctgcccggta tccatcagac
     6601 tttgcgccat cgggccgttg tcgacggaat aaacccacat gttgcgcttc accagttggt
     6661 aggtccagcc gcctgggcaa gcgcccaaat ccaccgccca catcccgttc gccaggcgtt
     6721 catcccactc atccgcagga ataaacacat gaaatgcctc ttccagtttg agcgtggaac
     6781 gactcggcgc atctgccgga aatttcaggc gcggaatgcc catatagaac ggcgaattat
     6841 tgttgctgta tgagtaaccg gtataacagc agcctggtgc aatgaagaat acatgcacta
     6901 ccggacgctt cggcgtttca tagttcgcca gcacccccgc atcgcgcaag gcagcgcgta
     6961 gcggcacggt gaatttacgg cagaatttca gcaactcttt gctttcgttg gtatcggcaa
     7021 cttcaacacg cagttcaccg cccttctcta ctacgccctg taacatgccg acaatggggg
     7081 taatacgatc ttctggcggc aaatgctgca ggagttcccc caccacaaac cactggcggg
     7141 caaaaattaa tgaactgaac ggcaactcac ggattaactt atcgccatca tcaggttgat
     7201 aacattcata aatgacataa cccgcattct ctttcacgcg ggcaaaaccg aaaatttccc
     7261 gctggccggc tttatcgcta atttctgcgg cgcactcttt ttcaaagccc ggacggcaca
     7321 gcaatacaac cttattcatg acttacgccc ttgcgcttta aacggatagc accaactaac
     7381 attaacgccc agcccgcaag gaagctcacg ccgccaaccg gagtgacaaa cgcccacaaa
     7441 cgcagatggg acagtgccag gcaataaagg ctgccgctga acagcaccgt gcctaacgcg
     7501 aggaaaacgc tactccagta aaaccagata ctgatgcgac gctgcattgc caccgccaga
     7561 cctaagatcg ccagcgtatg aaacgcctgg tattcgaggc cggtttggat ccagcccatc
     7621 tcaacggccc ccatggtttt acttaacaca tgcgcgccaa aagcgcccag agccacaaaa
     7681 atgaagccgc taatggcggc gaaaatcagc ataaaacggc tggtcatggt cgtaccctac
     7741 gtaaattatt gttcataacg aaagcggaat ttttcttgtt cagcagcggc tttcgccagg
     7801 atccattggc gaaaggcggc tattttaccc agttctgcct ggctgtcatg acaaaccaga
     7861 taaaaagcgt ttttactgac cagaacatca ttaaacgggc aaacaagacg tccggcttca
     7921 atttcagatt gcgccatcac gttatttgcc agcgccactc cctgcccgtg aatagccgct
     7981 tgcagcacca tggcgctatg gctaaaaatt ggcccttgct gaacgttgat atgatttaac
     8041 cccaattgac gagtatatgt ctgccagtca cggcgtgacg catcatgtaa taacgtatgt
     8101 ttagccagat cttccggggt cttcaagggt ttttcgccag tcagcagtag cggcgaacac
     8161 accggcaata aatattcggc gtacagtttt tccacccgta gccccggcca gttgccccga
     8221 ccataaaata tcgccacatc aacatcatcc gccagcttat cttcctgacg atcaaccgcc
     8281 tggattcgaa cgtcaattcc cggataagct gaattaaagc tggaaagtcg cggaaccaac
     8341 caatggatgg cgaaactggg gagtaaactg accgtcaacg cccccttggc gctacgggcc
     8401 tggagtttac gcgtcgcttc ggttaattgc gaaaatatct ctttgatatc gaggaaatag
     8461 ctttgacctt cctcggtcag caggagtgaa cgattacggc ggcggaacag ttttagcccc
     8521 agaaaatcct caagagactt gatttgatga ctgactgcgg cctgggtcac aaaaagctct
     8581 tctgctgcgc gagtgaaact taaatggcgt gctgcggcat caaaaactcg taaggcattt
     8641 agcggtggta atcgtttaga catggctttt aaactttgat gttaaatgaa tttaacaatt
     8701 agatcacact gtgtaaccta ttagtttttt taatctgagc cattataaat tgtccgttga
     8761 gcttctacca gcaaatacct atagtggcgg cacttcctga gccggaacga aaagttttat
     8821 cggaatgcgt gttctggtga acttttggct tacggttgtg atgttgtgtt gttgtgtttg
     8881 caattggtct gcgattcaga ccatggtagc aaagctacct tttttcactt cctgtacatt
     8941 taccctgtct gtccatagtg attaatgtag caccgcctaa ttgcggtgct tttttttacc
     9001 ttgcgatcgc gaattactga tccagttcga ccatctcttt cacgtcggta cggttgatct
     9061 gctgtttgtt gccattagcg tctttatacg aaatcatacc ggtatcgtta tcagtctgtg
     9121 gtttgccgtc agagacgatg gtacgtccgt cattggtgtg catcacatag ttcgaaccgg
     9181 aacaggcgct cagggcaaaa gtcagcatac aggcagaaat aattgcggca gtctttttca
     9241 tcatcttctc cttaaagcga ttaataaatc ttaatagtat tgattaacag gctaaaatta
     9301 acgcctaaca ctattcagca tatgttactg acgcggcttc gccaggatat ccagataatt
     9361 ctgatggtta gcactctcct tgtatcaaag tgaattttgc gtcacgatcg gtgcatcaag
     9421 ccgaggagta ccatgaacgt ttttaatccc gcgcagtttc gcgcccagtt tcccgcacta
     9481 caggatgcgg gcgtctatct cgacagcgcc gcgaccgcgc ttaaacctga agccgtggtt
     9541 gaagccactc gacagtttta cagcctgagc gccggaaacg tccatcgcag ccagtttgcc
     9601 gaagcccaac gcctgaccgc gcgttatgaa gctgcacgag agaaagtggc gcaattactg
     9661 aatgcaccgg atgataaaac tatcgtctgg acgcgcggca ccactgaatc catcaacatg
     9721 gtggcacaat gctatgcgcg tccgcgtctg caaccgggcg atgagattat tgtcagcgtg
     9781 gcagaacacc acgctaacct cgtcccctgg ctgatggtcg cccaacaaac tggagccaaa
     9841 gtggtgaaat tgccgcttaa tgcgcagcga ctgccggatg tcgatttgtt gccagaactg
     9901 attactcccc gtagtcggat tctggcgttg ggtcagatgt cgaacgttac tggcggttgc
     9961 ccggatctgg cgcgagcgat tacctttgct cattcagccg ggatggtggt gatggttgat
    10021 ggtgctcagg gggcggtgca tttccccgcg gatgttcagc aactggatat cgatttctat
    10081 gctttctctg gtcacaaact gtatggcccg acgggtatcg gcgtgctgta tggcaaatca
    10141 gaactgctgg aagcgatgtc gccctggcta ggcggcggca aaatggttca cgaagtgagt
    10201 tttgacggct tcacgactca atctgcgccg tggaaactgg aagcaggaac gccaaatgtg
    10261 gctggtgtca taggattaag cgcggcgctg gaatggctgg cagattacga tatcaaccag
    10321 gccgaaagct ggagccgtag tttagcaaca ctggcggaag atgcactggc gaaacgtcca
    10381 ggcttccgtt cattccgctg tcaggattcc agcctgctgg cctttgattt tgctggcgtt
    10441 catcacagcg atatggtgac gctgctggcg gagtacggta ttgccttgcg gaccgggcaa
    10501 cattgcgctc agccgctact ggcagaatta ggcgtaaccg gcacactgcg cgcctctttt
    10561 gcgccatata atacaaagag tgatgtggat gcgctggtga atgccgttga ccgcgcgctg
    10621 gaattattgg tggattaatg acgaacccgc aattcgccgg acatccgttc ggcacaaccg
    10681 taaccgcaga aacgttacgc aagaccttcg caccgttgac gcaatgggaa gaaaaatatc
    10741 gccagttgat catgctgggg aaacagcttc cggctttgcc agacgagtta aaagcgcagg
    10801 ctaaagagat tgccggatgc gaaaaccgcg tctggctggg atataccgtg gctgaacacg
    10861 gtaaaatgca cttctttggc gacagcgaag gacgtatagt gcgcggcctg ctggcggtgc
    10921 tgttgactgc cgttgagggg aaaaccgccg ccgaattgca ggcacagtcg ccgctggcat
    10981 tgtttgatga gctgggattg cgtgcgcaac ttagcgcctc acgcagccag gggctgaatg
    11041 cgttaagcga ggcgattatc gctgcggcga agcaggttta agcacagtac caggtcggat
    11101 aagacgttcg cgtcgcatcc gacctgatta tttacccctg acgagccgct ttcgccatca
    11161 tctttttcag cgcatgagaa accgcaacaa aaccaaaggt ggcggtcacc atcgtcgccg
    11221 cgccaaatcc agatgcacaa tccatccgct tcggcccttc tgccgtggct ttcatagcac
    11281 acaccgtacc gtctgactgc gggtacacca atgcttcggt agagaacacg caatcgacgc
    11341 cgagcttacc tttactgttt ttcactacgc caaaatcgct tttcaggcgc tcacgcaact
    11401 tcgccgccag cgggtcctga atcgttttcg ccagatcggt aacctgaatc tgcgtcgggt
    11461 caatctgccc acccgcgcca ccggttgtga ccagtgggat tttattgcgc cgacaataag
    11521 caatcagcgc cgctttgggc cgtacactat caatggcatc aatcacgtac gaataaccca
    11581 cgctcatata ctgcgctacg ttatccggcg tcacgaaatc atccaccacc gttacacggc
    11641 actccgggtt aatctggcga atacgctccg ccataacttc cgcttttgcc agcccgacgt
    11701 tatcgcgcag agcgtgaatt tgccgattgg tattggtgac gcacacatca tccatatcga
    11761 taagcgtgat tgcgccaatc cccgtgcgcg ccagcgcttc cgccgcccag gaaccgacac
    11821 cgccaatacc gaccacacaa atatgcgcgt cagcaaacag ttgcagcgct ttttcaccat
    11881 acagacgcgc tgtgccacca aaacgctgac gccatgcatc actaattacc acagacatag
    11941 cacctcagaa taaaaagggc gaggttatcc ctcaccctaa acgcataaat gcctgatgcg
    12001 ctacgcttat caggcctaca taactcctgc aatattttgg atttgcatga tttgtaggcc
    12061 ggataaggcg ttcacgccgc atccggcatg aacaacgcgc acttagtcaa taatcaggat
    12121 gtgaggttac ccctcaccct gtcatatccg taaaaacggc atacagaata tcacatcagc
    12181 cactaaagac gttacctgcg cccggagccg ttttcagcac ccaaacccgt ccatagtggt
    12241 tgtaccaacc tgcgcggtgt ccggcttccg gcccgatccc ttgatagata tcgaagtgtt
    12301 ggcctttgat tgcgccaccg acatccagcg ccaccatcag acgcaattcg tactgaccgt
    12361 taaatttgcc gttattatcc agcagcggca cttctgccag caaggttgta cctggcggaa
    12421 taatggaacg atcagaggca actgaagcgc gaccaaccag cggcaccgca cttgcccctt
    12481 tcaccggtgc aaaagattgc ggtttaaaga agacaaaaga cgggttctgt tccagcagct
    12541 cgcgaacctc ggcttcactg tgtgtttcgc cccagtgacg aatcgcctgc atcgacatat
    12601 cttctttttt cacttcgcca cggtcgatca gcaccttacc aatgctgcga taggcatgac
    12661 cgtttttccc tgcatagctg aaaaagttaa gcggactgcc atcaccaaag tcgatatacc
    12721 cactaccctg cacatccata atgaagttat ccatcaggga gttactgtaa gcgagaatat
    12781 atttatcact caatgccccc gcgtagatct ccgcacgaga cggcagacga ccacgttttg
    12841 gcggcatacg gtaaatagga tactggaact cgccctggcg ggtatggcgc gcctgaatta
    12901 ccggcgtgta atagcctgta aactgcacgt ttccatagtt gtcggcacct tccatctgcc
    12961 aggcatcaat gccgaactgg cgcatattgc gggtatcacc gcctgcgcgc agccactctt
    13021 gcaccgcgtt ataaacatta ctctggttgc catacagacg cggtgacgaa ttacggatat
    13081 ggttaatttg ctcggcaaaa tcaccggcgt taatcggcgc gccaacggca tctggctggt
    13141 tcaccagaga gaaaggctgg gtaaatttcc cgtctttata ttgctgtccg cgatcggttg
    13201 gtttggaaga gcaggcggca agcattgcca caaccgtgcc cataagaagg tactttaccc
    13261 aacgtccttt cattgttctc ttcttcaggt taaaaaataa ggcgcaacga agataacaaa
    13321 ccgccgcgtt taatgaaatg agcagcctct ccctgacgca aattttgcac aaaaaatagg
    13381 ctttagtgat ttgtttttgt tcaaaatcat gccaaatccg tgatcggggt aaaaaaaagg
    13441 ttgcatgaaa acgcgagcgg agtatagtgc gcatccacgg acgcggggtg gagcagcctg
    13501 gtagctcgtc gggctcataa cccgaaggtc gtcggttcaa atccggcccc cgcaaccaat
    13561 tattgaacac cctaacgggt gtttttttgt ttctggtctc ccataaaaaa gcgccattca
    13621 gcgccttttt atcatcccct tctcgccagc gtcgccccat cggcaaaata cgctttaatc
    13681 cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct
    13741 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
    13801 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat tttaccgagc
    13861 ttattcagca ctgctttacc aaacttcagg ctgtcggcaa tggtcagcga ctgcaccata
    13921 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc
    13981 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcacc tttggttgag
    14041 agcgcaaaca cagaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag
    14101 acaaacaggt cagcacgctg tttctgagct tttgctacgc gcacttgcaa cggaatgaag
    14161 atgtcttcat tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc
    14221 agacggcgag ctatttgcaa taccacgtct ttttcgcgcg ttttgtattt ccccaccgca
    14281 ccggagtctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
    14341 gctttacctg gttgtggacc actttgcgct ggtggcacct gcttttcgag gtcgcctttg
    14401 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
    14461 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
    14521 tttacgtttt gctttaattc aaaaaccatc cgtacggttt gcgggtcaaa ttgcccgacg
    14581 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
    14641 gagttcaggt ttacatcttc gatatccacc accacgcgtt ccgggttact caacgcgaac
    14701 tgcttatatt tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc
    14761 cagacgcgca ccgccacgac ctggctgacc gcagccagac tgacctgact tacgctcaat
    14821 agccacatgg cacccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
    14881 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
    14941 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
    15001 atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
    15061 cgaaaaaaca gaataaaaat acaataattt cgaataatca tgcaaagagg tgtgccgtgg
    15121 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
    15181 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
    15241 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
    15301 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
    15361 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
    15421 gaacattgca actggatatt actgctcgcc tgtcgatgag tctcaataac acgccgctac
    15481 agggcgcgca tatcaacgtc gtcagtggca attttattat tgcccagccg ctgggcgtcg
    15541 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc
    15601 atcgtcaact ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
    15661 gcgagagctt taacctgacc tcggaagaga ttgccactca actggccatc aaactgaaag
    15721 ctgagaagat gattggtttt tgctcttcac agggcgtcac taatgacgac ggtgatattg
    15781 tctcagaact tttccctaac gaagcgcaag cgcgggtaga agcccaggaa gagaaaggcg
    15841 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
    15901 tgcgtcgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
    15961 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
    16021 caatcaacga tatcggcggt attctggagt tgattcgccc gctggagcag caaggtattc
    16081 tggtacgccg ttctcgcgag cagctggaga tggaaatcga taaattcacc attattcagc
    16141 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
    16201 aaatggcctg tgtggcagtt cacccggatt accgcagctc atcacggggt gaagttctgc
    16261 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
    16321 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttattgc
    16381 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
    16441 tagggtaagc gatgaaaatc gccggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
    16501 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
    16561 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac ggcgacgcgc
    16621 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
    16681 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
    16741 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
    16801 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
    16861 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
    16921 cggattacga tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc
    16981 agccacgcca aatggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg
    17041 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc
    17101 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
    17161 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
    17221 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
    17281 gagactgtcg cgcaaagatg ccgcttctgt gccagttcct gccggaacgt gagtccccgt
    17341 caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat
    17401 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
    17461 cactcgcgca tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
    17521 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
    17581 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
    17641 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
    17701 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
    17761 tgccatttga attaacgctg ccagcaactt cgctacggtg gtcgttttac cggtgccagg
    17821 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgccgcaa ctttttgcca
    17881 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta aagcttcatc
    17941 aacctcaatg gcatgattca cttcgttgaa aaagcgtgcc actgtgcgct cgttacacca
    18001 catgcgattc aaataaagac gatcaccaca gaggatcatc ggcgtgggtt catctccccg
    18061 gctgaccgct tgtgaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
    18121 gacacaggtc gccaacagcg gatgcgacgc ctcattattt tccagtcgtg aaagcggcaa
    18181 acaaacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
    18241 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
    18301 ctccacagct tccagtaatt gcttctgcaa tttcattacg cctcctccag ggtcataccg
    18361 gcaaacatct catccatcag ggtaatcaac tcggcgttgg ggcgagttgt gtaaatcccc
    18421 tgttgcggat gttctttatc aacgccacgc aggaacagat aaataacgcc gccaaagtgg
    18481 cgctcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa
    18541 agctgatatt gcagatcata gcggtgtgcc tgcattgccg ctgccatagc ctgttgggtg
    18601 taagccgaac tgtcttcacc caaccagttg gatttatagt cgagcaggta ataacgccct
    18661 tcgtggcgga acaccaggtc gataaagcct tttaacatgc cacgtacctg catgaactcc
    18721 agcggcgggc agcctgcgga tagcgggtca aactggcgga ttaacgtatc aagctgactg
    18781 gcgataagcg gttcactaat cggcagataa aactccatct ccacctgttt attgcgggcg
    18841 gaaagttgac tcaggcttac gccggtttca ttgagaggtg cctggaggac agccgtgatc
    18901 cactcggtca ataccggttc ccactgcgat tcaaagccgc cgagttccag tttttcccgc
    18961 acccagttcg ggtcaaccgg ctgggtaaaa tccaggtctt caaacaaact gtgcaagaac
    19021 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
    19081 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
    19141 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
    19201 aacgtcttcg cattcagctc tgctgtagaa acatcattaa cctgccaggg ttggttatca
    19261 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
    19321 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
    19381 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagacta
    19441 caatgccaaa ccgaacgcgt cagcgccaca taaagcaaac gcagatcttc cgccagacgt
    19501 tcggcctccg cgagatcgac actttctggc gcagcgttaa gatccagaac agcctcaaac
    19561 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
    19621 cagacaagcg aatattccag cccttttgat ttatggatcg tgacaatctg caccagatgt
    19681 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
    19741 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgtagc
    19801 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
    19861 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
    19921 cgccagattt gccgataacc atcgaactct tcgaccaccg cgtcccacgc atgttcgtca
    19981 ttattcagcg tttcgatatc cagcgcgttc agccccatca ttgacgttgc cagcgcacta
    20041 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
    20101 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
    20161 aacgttaagg catcgcgcac ctgggcagcc tcctggcggc tgcgcaccag cacactgatg
    20221 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
    20281 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
    20341 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
    20401 cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
    20461 atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac gctgttcacc
    20521 attcctggtg cggaacgcca gttggtgtct aaagtgtagt gggcgtgaac ttcgctacgc
    20581 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
    20641 ccaattagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
    20701 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
    20761 gccgctgcca acacctcacc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
    20821 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
    20881 gccaatgcgc gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
    20941 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
    21001 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
    21061 gcccaggcgc tgatcttgtc gatccattta gcctgattgc tacggttaaa cttgcgtcga
    21121 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
    21181 tttaccgtat caatacgcgc cacaatttgc gcgtgacggg aagccagcgt ttcatcatcg
    21241 ggcggcggtg ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgctcagc
    21301 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
    21361 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagccgaga ctcatcttca
    21421 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
    21481 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
    21541 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca gacgttcgta cagtggattg
    21601 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
    21661 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacggtcagc
    21721 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
    21781 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
    21841 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
    21901 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
    21961 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
    22021 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
    22081 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
    22141 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
    22201 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
    22261 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
    22321 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
    22381 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
    22441 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
    22501 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
    22561 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
    22621 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
    22681 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
    22741 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
    22801 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
    22861 ctgatcttcc gtagcggtat agctaaagta tccctcgagc aatgcctgga acagctgcgg
    22921 cagacgctgg gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa
    22981 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
    23041 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
    23101 aatcaggctg acatcagctt tgggttcgct ggcaaaataa cggcttggcg catacaccac
    23161 gcgcagattc gactcatcaa caatcagctc tggatggtca tatttcttct ctgacttaat
    23221 cagcgagaaa tcgtcaggaa tataggggtt aagctccggt aaggagagcg caatactggc
    23281 agctttttgc tgccagtcag cgaaagtttg ttcgctgatt ttatcgacct gatacggcgc
    23341 atcgacaaaa taagccgttt tattgtgcgg ctctttcggg ctgatatacc agatacgcgc
    23401 attctgcggc gtcatcatcg ccagacgttc ttttactgct ttagcatcgt accgatcggc
    23461 aatattgact gcatccagcg tatgctcaac cggaacgcga atcatggtat ctgccagcca
    23521 ttcgacgtaa tccatatcac gggtgattga cggataacgg aagtcgatat ccagcacatt
    23581 cgccagttca tcgaagtatt gtttatcgat ccctttttca cgtaacaaat tgaggtagct
    23641 aaaaatagcc gccacaacct gatcgcgatt cgccagacct ttatcggtta aagacgcaga
    23701 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    23761 aactaacccc tgcttttgca gccagtcaga cagtgtacct gggctgcgat taccaatcag
    23821 ataggtaatc aattcatccg ttttactgcg gaacttcgct gagttgttat cgatgcgaaa
    23881 ctcaacgcgc agaactttac gcggcagcgc tgggacgtaa tgaatgataa tgcccttttg
    23941 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    24001 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    24061 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    24121 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    24181 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    24241 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    24301 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    24361 aacttccaga tagaaagccg tgcgatacgg tgccgtgctg gcattgtgac taccgccgtg
    24421 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    24481 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    24541 gggcaccacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    24601 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    24661 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aatggtgccc aaagggcaac
    24721 taacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    24781 ttattttctg gccagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    24841 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    24901 taagcggata gattgcgcaa tttttataca gcgctcatga ctgattaaag cgaaacagcg
    24961 gtaacaggaa acgttgcgac tgttcaacga ttgcctccat tgtctctggt gttaattgcc
    25021 gccagagcct ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    25081 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    25141 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    25201 atggcgcgga catcccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    25261 gttcggctgc aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt
    25321 caccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    25381 ccacacttaa taaagaggga cgccagcgca acaagccatc cggctgcacc tgcggcaacc
    25441 aaccagttat ctgcacaccg ttgcaagcga gatcaatttc catactctgc cccggctggc
    25501 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    25561 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    25621 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    25681 gtccttccag aataaatggc tcggtgtcgg ggatttcgct gtcttcagta cggaagttca
    25741 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    25801 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    25861 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    25921 gatagttttg tggatcaaac ggcattcggg tatggaggca agtaagatgc gcttttaccc
    25981 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    26041 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    26101 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    26161 gatagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    26221 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    26281 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc gccccggcga
    26341 gaaaacgctg gctgatacgt tcttgatcca ggcgctgtgc cagttcatca cgcaatagtg
    26401 acagcggcac cgcatcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    26461 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    26521 gcatatcgcg acaaaccggc aaccactctt ccagcggacg ctcctgtgcc agcccgcgac
    26581 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    26641 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    26701 agcccaacaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    26761 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    26821 gacgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    26881 ccagtaatgc cagcacgtct tccgacacaa agcggctgtc tggcaacgat aacaggctga
    26941 taaacgcttc aagtacagga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca
    27001 ggtaacgatc cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg tagctgtcga
    27061 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cgggtcttct tccagcatcg
    27121 ccagcaggcg atcgtgcaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg
    27181 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    27241 tgttcacacc agcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    27301 gcaggttatc tggcgtgaca tcgacaaagg cgtccagctc ctggctgctc tccaggtcag
    27361 aaaggagata aatgtagtcg cgcccaagct taccccatga agccagcagc gggttgccga
    27421 catcctgttc accatcgctg ttaaagagct gcccggcatt ttcgctatcg cgaaatagcg
    27481 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgggt cagcagtttc gccagataag
    27541 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    27601 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    27661 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    27721 cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg ttgcccgagt tcatgggtat
    27781 attccaccag agccttccac aacggcgctt gccaggcctg tgcttctccc agcccttcaa
    27841 ccaaatgtcc tgtttcccat tgtgccagcc actccgggcg atagaccaga tactggtcaa
    27901 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    27961 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    28021 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatctcc ggtaacaccc
    28081 ggacgaacat atcccagata aagctcgcag gcagcggaaa atcaatgttt gccgcaatac
    28141 caaacttttg cgacagggtc atttgcagcc actgtgccat accggtactt tgcaccagaa
    28201 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    28261 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    28321 gacgattcgg gcaatgcagg cgggtcatct cgcctgctct gcccccgggt gaaactagcg
    28381 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    28441 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt
    28501 actggtttct gctggcaaga ctgttcatta atgttcgctg gaaacccgat aacgcagtga
    28561 caatcatcac catcaacacc atcgccaaca ttacctccgg caggctaaag ccttgttgat
    28621 tcttcaggga agctgacata acgccccctc tttcagcgga caaaaatcgc tccagccgcg
    28681 tggcgaaaaa acaatttttc catcgatgac ttcacctgct cgccacaacg aaacgccttc
    28741 ataaccggca atcaataagg cttcattatc tgccaataaa cgcaaacaaa cccgggcatc
    28801 ggttccagcg tactgcaagc actgaactgc tggctgcgtc tgccaggagt acatttttcc
    28861 ccacgccagc gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac
    28921 gcgagaggca aaactgcgat cctgttggct cattccttgt aatagcaagc tacccaaaac
    28981 tagcagcatc aggaccagag ccagtgacga aacacccttt tcgcggttca caggttgaat
    29041 cctgtcacgc tatagctggc attcaccaca gtttgcggtt cagacttact ggcagcacgc
    29101 atattaaccg tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg
    29161 tcgataatga tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc
    29221 gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcggactct
    29281 tttaccggtt cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgacacag
    29341 tcaccctgtc cgacaatttc cagcccttcg ccggtacaga tgccatgaca ataacccgcc
    29401 ctctggagat gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagtgccagc
    29461 ttacgggtgc tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccaac
    29521 aacaatacgc tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct
    29581 tttacaggca tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa
    29641 ccaccagcca ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat
    29701 tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc
    29761 gtggcacaaa gaccaatggt gaactgccat gacaggtatt cgccccagca acggaactca
    29821 caaggcacca taacgtcccc tccctgataa aactgatact gtggtcgcgg ttatgccagt
    29881 tggcatcttc acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccaaagcc
    29941 gttgcgactg ctgccagtat tgccagccat agagtccact tgcgcttagc gtgaccaaaa
    30001 tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag
    30061 tatggagcga ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg
    30121 actatttacg ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaat
    30181 ggagggtaaa aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag
    30241 atagccaccg gcgctttaat acccggatgc ggatcgtagc cttcaatctc aaagtcttcg
    30301 aaacggtagt cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc
    30361 ggttcgcggc ttaattgcag atgagtttga tccatatgat tgctgtacag gtgcgtatcg
    30421 ccaccggtcc agacaaaatc acccacttcc agatcgcact gctgcgccat catatgcacc
    30481 agtaacgcgt agctggcaat gttgaacggc aggccgagga agacgtcaca ggagcgctga
    30541 taaagctggc aagagagttt gccgtctgcc acatagaact ggaagaatgc atggcacggt
    30601 gccagcgcca ttttatccag ttcgcctacg ttccacgctg aaacaataat acggcgggaa
    30661 tccgggtcgt ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatcc
    30721 ggcgttggcc aggcgcgcca ctgtttacca tacactggcc cgaggtcgcc gttttcatcg
    30781 gcccattcgt cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc
    30841 tgcagaaacc acagcagttc atggatgatg gaacgcaggt ggcaacgttt agttgtcacc
    30901 agcgggaatc catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg
    30961 gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa
    31021 tactgtttca tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc
    31081 atcatgatca cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac
    31141 tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga
    31201 aacgcgccgt agccaatcag gaacaagcct gagacagctc ccattggccg cggtttacga
    31261 atatacaggt tgaggataat aaacagcacc acaccttcca gcagcagttc gtaaagctgt
    31321 gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt
    31381 tgcagcagca aaatatcttc tgtacgggag cctgggaaca gcatggcaaa cgggaagttc
    31441 gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcgccaaga
    31501 ccaaacggaa tgagcggtgc tataaaatca gatacctgga agaaggaacg tttggtgcgg
    31561 cgggcgaaga taatcatcac cacaattacg ccaatcaggc cgccatggaa ggacatgccg
    31621 ccgtcccaga cgcgaaacag atacagcgga tcagccataa actgcgggaa gttgtagaac
    31681 agaacatacc caatacgtcc gccaaggaaa acgccaagga agcccgcata gagtaagttt
    31741 tcaacttcat ttttggtcca gccgctgccc ggacgattcg cccgacgtgt tgccagccac
    31801 attgcaaaaa tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct
    31861 attgagaaaa tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac
    31921 cacaagttct tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc
    31981 aggcgctcca aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt
    32041 aaaagatatg tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga
    32101 cgctccataa aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta
    32161 cgctgcgcaa gattttctgc ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc
    32221 gctacagaac gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg
    32281 cacatgggat cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct
    32341 tcccgggcga tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc
    32401 gccacccggg tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca
    32461 acagagatga aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggtact
    32521 tccagcatga tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca
    32581 cgtccggcac gttcaatcag gcggcgagct tcgtcaactt catcgaggct ggtgaccatc
    32641 ggcaacagaa tattcaggtt gcccgtagcg gcgttagcac gcagcatcgc ccgcacctgg
    32701 atcaagaaga tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga
    32761 ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc
    32821 aaggtgacgg gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct
    32881 tcttccgacg ggaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg
    32941 ccatcaatac ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg
    33001 acttttatac gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga
    33061 cggctaagct caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc
    33121 ggatcgacca gcaattcacc gcgatagcca tcaacgatca gcgtcctacg atgcagcacc
    33181 gaaggctgaa tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca
    33241 gcatgggagt tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc
    33301 tcagcaagag ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg
    33361 ttcggccctt gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa
    33421 tcgccagcgc gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt
    33481 tcaatgaccg tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat
    33541 tcgcgacgca gccgggtgtc cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt
    33601 tctttttgtg caccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct
    33661 tccagcgccc cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac
    33721 acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt
    33781 gctgccggta atgcgcggat tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc
    33841 gactgagaaa gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt
    33901 tcgtcatact ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt
    33961 tgaataattg gtacgcctaa aaacgcgcgg aagcgttctt cttttacgga ggggatgtat
    34021 ttgaagcagg ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc
    34081 aggccgacga tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc
    34141 ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag
    34201 acctcggtat ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg
    34261 cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg
    34321 acttaacctc ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc
    34381 agtgacatca ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat
    34441 gacaccacct gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt
    34501 gtactgctgg tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat
    34561 ttttgttttt ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat
    34621 ttgtagcgca accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct
    34681 acttcttcaa acaattcacg gtacatcgcc tgctctgcgg attctccggg gttaattccg
    34741 ccttgcggaa attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg
    34801 cgattacaaa tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta
    34861 cctcaatata aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca
    34921 ctctctgcag ggcattacac actaataaca attgaataac tcacagttat gtgcagagtt
    34981 ataaacagag gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg
    35041 aagaactacg gaattactac gggaaaaccc ggagcattct gaataagccc gatatagaca
    35101 ttttaaaata ttcattatca gtcaatgcat tacatgtaat ttaagtaatg aaaaagagta
    35161 attcgtgacc caggtcacac ctctcattta cgggttggcg aaagatcaac caatgccgta
    35221 tttatccaca gaatgtgcca ctaagttaag cactgaacca ccaaaaactg gagtttcgtc
    35281 gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc
    35341 tgtggataac atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacccgtt
    35401 gcagtgttgc gcaactcgat taccggcaac ctaaaaagca atataaatca gagaattgaa
    35461 caacgcatgt ggaaaaagtt acactgcgaa tattcggcac ataattgctg tttgtttttt
    35521 aaccaaggta tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag
    35581 aacagttgtt agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac
    35641 ttgccgggct ggttacgcca gagaatttaa aacgcgataa aggctggatt ggcgtgttac
    35701 tggagatctg gctaggtgcc agcgcaggaa gtaaacctga gcaagatttt gctgctctgg
    35761 gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg
    35821 tttgtgttgc cccgttaacg ggcaatagcg gggtgacctg ggaaaccagc cacgtgcgcc
    35881 acaagctcaa acgcgtgctg tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc
    35941 agcgtcgcgt tggatcaccg ttgctgtgga gcccgaatga agaggaagac cggcagctac
    36001 gcgaagactg ggaagaatta atggatatga ttgttctcgg tcaggttgag cggattaccg
    36061 ctcgtcacgg ggaatattta cagatacgac cgaaagcagc gaatgcgaaa gcgcttaccg
    36121 aagccattgg tgcccggggc gaacggattc tgacgctgcc acgcggcttt tatttgaaga
    36181 aaaatttcac cagtgcgctg ctggcccgtc attttctgat ccagtagcca tcgctttgac
    36241 ctgccgcttt ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt
    36301 atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga
    36361 gatcgttctt gggatcgaca atattatttt cctttctctg gtggtggcaa agcttcccac
    36421 agcacaacgt gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc
    36481 gctgctggca tcaatcgcct gggttacgcg cctgacaaat ccgcttttta caatattcag
    36541 tcaggaaatt tctgcccgtg acttgattct gcttctgggt ggcttgttcc ttatctggaa
    36601 agccagcaag gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt
    36661 ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg gatattatct tcagcctcga
    36721 ttcagtgatt accgctgtgg gtctgtcaga tcacctgttt attatgatgg cagccgtggt
    36781 gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca
    36841 tccttcggta aaaatgctgg ctctctcttt cctgattctg gtgggcttta ccctgattct
    36901 ggaaagtttc gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat
    36961 tgcggttgaa agcctcaacc tgattcgcaa caaaaagaat ccgctctgat actccgtacg
    37021 ctctcctgcg ggagggcgta ctcactcctg ttaccctcac agtttaagat tttcctgctt
    37081 tcaagatata taacgtcggt ttataaacag actattatca taggtgggcc aaatgaggat
    37141 aaccggatga aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc
    37201 gggtgttcca gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga
    37261 aaacctgaaa ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg
    37321 atgcaaatta accgtgatga tgtttcgcaa ataattgaac gttaacaaat aaggtcagca
    37381 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
    37441 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
    37501 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
    37561 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
    37621 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
    37681 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
    37741 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
    37801 tccgcgaagc gctgcatgac agcctcaagc gtctgcagac tgattacctc gatctttatc
    37861 aggtgcactg gccgcagcgc ccaaccaact gcttcggcaa actcggttat agctggacag
    37921 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgag
    37981 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
    38041 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
    38101 tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac
    38161 tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
    38221 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
    38281 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggcctg gatcctgctc
    38341 agatggcgct cgcttttgta cgtcgtcaac cgtttgttgc cagcactctg ctgggcgcaa
    38401 ccacgatgga gcagctgaaa actaacgtcg aaagtttgca tctggagttg agcgaagacg
    38461 tattagctga aattgaagcg gtgcatcagg tttatacgta tcctgcacca taagattaac
    38521 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
    38581 tgccagcgca aatagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
    38641 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctattttcgc caaggttttg
    38701 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
    38761 gagcggaacg acaaaaaagc cccccagcac gccaatcagc atcaacaagg cataggctgg
    38821 cagcagctcg tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa tcccggctgg
    38881 catacagcgt gacacggttt ccagcgtaac cagcttcgct gccgcacctg cgccaaccac
    38941 gataccaatc gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatgcccag
    39001 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
    39061 actggtgccc accagcgaaa aacgcgtttc accattgcac catagcgagg tgcaggcatt
    39121 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
    39181 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
    39241 ggcgaccagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
    39301 cgctatggta gaagcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa
    39361 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    39421 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    39481 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    39541 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    39601 atagaactgc gctttcagta acgccagagt ggcaaacagt agtgcattat cgccaaacgc
    39661 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtattagt
    39721 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
    39781 agtcaggttt gccgctgcca agtaatggca tctgtttcag ataacgaata tcacgcggta
    39841 cagcaagctc cggcacaccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
    39901 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaattgcag
    39961 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    40021 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcgacc ctgaatctgc acaaagccct
    40081 gctcgtcaaa acgcacgata tcgccagtgt cgtaccagcc gcgctccatt tcgccgcgca
    40141 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    40201 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccagga accgacaaca
    40261 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    40321 cgttgataga aacgacaggc gcgcactcgg ttacaccata gccttcaagg atgcgcaggc
    40381 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
    40441 catagcgtag acgatagaaa tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    40501 tggaggtacc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatgcggt
    40561 aatgcagcgg gcttggataa aggaacactt ctgcacctgt gagcagtggc gtaaacaggc
    40621 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
    40681 tgaagtcggc aatcgtttta atctgctcga cattcgccag aatgctttta tggctatgga
    40741 cgacgccttt cggatggcct tcagaaccgg aggtaaaaag gatcagcgcc tcttcttccg
    40801 gctgctgttt aacctgtgcc agacgcggca tcagcaaatg agcgaagatc catactttgt
    40861 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    40921 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
    40981 tgatttcagc cgccgtaatg gcgctggtca gcccttttac cccagcggtg tagttcatca
    41041 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    41101 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    41161 caaacagcgt tttagtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
    41221 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
    41281 cacgcgggcg caccgccata cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    41341 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
    41401 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    41461 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    41521 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
    41581 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
    41641 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    41701 gttgacttat tgaggtgtaa acggcaaata ccggacgtac gggtaaaaac aatgccagca
    41761 gtatgccatc aataaaagag acgtgattag gcgtgattag aacgcgctcg cccttcaatg
    41821 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    41881 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    41941 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    42001 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    42061 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
    42121 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
    42181 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
    42241 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
    42301 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
    42361 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccacccc ttgaaccaac
    42421 gggcgttttc cgtaacactg aaagaatgta agcgtttacc cactaaggta ttttcatggc
    42481 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
    42541 taataattca cccaaagcca gcgaagcgtc ccggctggct gtgcatagtg caatggagtc
    42601 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
    42661 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
    42721 acaggtggct tatcacaccg gtaatttctt attgattggc aacggttacc acaacgaaca
    42781 aaaagagcgt caggctattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
    42841 tgccaaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
    42901 gctgatcaac cgtatcctgc ctggctttga aaaccgttgt attgctcttg acgatcgtta
    42961 cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt catacccgca ttggttatct
    43021 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
    43081 tgctgaaagt ggtattccgg ccaatgatcg gctggtgaca tttggcgaac cagacgaaag
    43141 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
    43201 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
    43261 tgtaccaggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
    43321 accgcgcctg accaccgtgc gttacccaat cgtgacgatg gcgacccagg ctgccgaact
    43381 ggctttggcg ctggcggata atcgccctct cccggaaatt actaatgtat ttagcccgac
    43441 gctggtacgt cgccattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
    43501 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
    43561 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt
    43621 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg
    43681 ccttcacttc cggcaaggcg cgggtttcga catttccccc ttcctgctgg gtaaagacat
    43741 cgcccgattc acataacggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
    43801 aacgaccatc agctgccaga gcactgatat ggtggtaact accgtacatt gctgggcgca
    43861 tcagatcgtt gaacccggca tcaacaagca caaagtggcg gctacccatt tgtttgacgc
    43921 tccgcacctg agtaattaat acgccagact gcgctaccag gaagcgaccc ggttcaattt
    43981 ccagtttcac agggtggccc aaatggcggg caatttgctc acgcgcggca ttccacagac
    44041 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaaca gaaagcccac
    44101 cgcccgcaga aatagcctgt aaatcctgac cgaattcgag gacctgacgc accatagcac
    44161 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
    44221 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
    44281 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
    44341 ccgggttaac gcgcagccat acccgatgcc caggcgaaac ctggcccagt tggtcgagca
    44401 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
    44461 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
    44521 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
    44581 gcattaagcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
    44641 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
    44701 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagagcggta tcggtgctga
    44761 acagtgaatg tggcatagca aactccagat aagtgctttt ttatgattac gccacatcat
    44821 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg
    44881 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
    44941 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
    45001 tcagccgcga actggcgcgc tttgagaagg tgatcgggct gaaattgttt gagcgcgtac
    45061 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
    45121 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
    45181 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
    45241 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
    45301 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
    45361 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
    45421 gtcatccact ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
    45481 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
    45541 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtgtgc gcaatggtac
    45601 gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg
    45661 gtttagtggt gcggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
    45721 tgcatcgccc gtcatcagcg ctggtgcagg cgtttagtga gcatttacaa gcgggtttac
    45781 cgaaactggt cacttctctt gacgcgattt tgtcgtcagc tacgacagca taaaagcgac
    45841 agcatcctcg gcatggatcg ccgcggtatc aaacacaggc aaaacactga gctcttctgg
    45901 caccagtaaa ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag
    45961 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
    46021 ttcttcaaaa ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt
    46081 gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
    46141 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
    46201 aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt
    46261 attggtacat aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc
    46321 cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa
    46381 atcgacgcta tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc
    46441 ttcatttatc agacgatagt aaggaatagt ggattcccag ctcattcctc ccagcaaacc
    46501 aattgttttc attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga
    46561 gacaaacgcc tcaacggcca ggtgcccaat ctctattaac gaaaaaaggg ccggatgtac
    46621 agcacatccg gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt
    46681 ttgcgttcga tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtgatg
    46741 cctacaaacg caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgcta
    46801 tcaagtagtg tcaggaaggt cgcgccgata atcatattcg acacccagtt tgtcgtcgtt
    46861 gagcaggtga tgccaaaatc gcggcatttc agcggctgaa tttcagagca caggatccac
    46921 accactggcg cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag
    46981 agccaggaca agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc
    47041 accagagtgc ctaacgccat cacgctgaaa ccaattttca gagccggttt acgccctgct
    47101 ttatctaccg taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga
    47161 gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgcg
    47221 tagtacatga tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga
    47281 aacacagcac gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ctgttttaac
    47341 ttcaggcttt cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc
    47401 atacgcaaca cttcttccgc ctcaatatga cgcccctttt ccgctagcca gcgcgggcta
    47461 tttggcagga agaccaccag aataatcagc aggactgctg gtaaagcaag aacccccaac
    47521 attgcgcgcc agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg
    47581 ccgagtgtga ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc
    47641 atttcagaaa gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc
    47701 acacgagcgg cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt
    47761 acaaacagga tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa
    47821 ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc tactaaccac ccattcctgc
    47881 aaacgactgg tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg
    47941 atatcaagac caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata
    48001 cgccgcgtat cccgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt
    48061 ttttcctgcc agcagagagt aagacatagt gaaaaaatac gtgaacaact cacacaggtg
    48121 tcaggtcgga aacagcataa atatggatta aattgctacg acatgtcgtt atgtgatgga
    48181 tattcc
//
LOCUS       DAZKFU020000007        44473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZKFU020000007 DAZKFU020000000
VERSION     DAZKFU020000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 44473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.007"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(342..884)
                     /gene="ycfP"
                     /locus_tag="ACHOQQ_RS01545"
                     /old_locus_tag="pgaptmp_000309"
     CDS             complement(342..884)
                     /gene="ycfP"
                     /locus_tag="ACHOQQ_RS01545"
                     /old_locus_tag="pgaptmp_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGU4468616.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(907..1932)
                     /gene="nagZ"
                     /locus_tag="ACHOQQ_RS01550"
                     /old_locus_tag="pgaptmp_000310"
     CDS             complement(907..1932)
                     /gene="nagZ"
                     /locus_tag="ACHOQQ_RS01550"
                     /old_locus_tag="pgaptmp_000310"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGU4468617.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(1943..2767)
                     /gene="thiK"
                     /locus_tag="ACHOQQ_RS01555"
                     /old_locus_tag="pgaptmp_000311"
     CDS             complement(1943..2767)
                     /gene="thiK"
                     /locus_tag="ACHOQQ_RS01555"
                     /old_locus_tag="pgaptmp_000311"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGU4468618.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLHDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENAEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2748..3389)
                     /gene="lpoB"
                     /locus_tag="ACHOQQ_RS01560"
                     /old_locus_tag="pgaptmp_000312"
     CDS             complement(2748..3389)
                     /gene="lpoB"
                     /locus_tag="ACHOQQ_RS01560"
                     /old_locus_tag="pgaptmp_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGU4468619.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3403..3780)
                     /locus_tag="ACHOQQ_RS01565"
                     /old_locus_tag="pgaptmp_000313"
     CDS             complement(3403..3780)
                     /locus_tag="ACHOQQ_RS01565"
                     /old_locus_tag="pgaptmp_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGU4468620.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3783..4142)
                     /gene="hinT"
                     /locus_tag="ACHOQQ_RS01570"
                     /old_locus_tag="pgaptmp_000314"
     CDS             complement(3783..4142)
                     /gene="hinT"
                     /locus_tag="ACHOQQ_RS01570"
                     /old_locus_tag="pgaptmp_000314"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGU4468621.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4476..6665
                     /gene="fhuE"
                     /locus_tag="ACHOQQ_RS01575"
                     /old_locus_tag="pgaptmp_000315"
     CDS             4476..6665
                     /gene="fhuE"
                     /locus_tag="ACHOQQ_RS01575"
                     /old_locus_tag="pgaptmp_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HGU4468622.1"
                     /translation="MLSTQFNRDNQHQAITKPSLIAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQTTLNATHSEVE
                     FDSKMMYVDAYINKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGRYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(6725..8158)
                     /gene="ptsG"
                     /locus_tag="ACHOQQ_RS01580"
                     /old_locus_tag="pgaptmp_000316"
     CDS             complement(6725..8158)
                     /gene="ptsG"
                     /locus_tag="ACHOQQ_RS01580"
                     /old_locus_tag="pgaptmp_000316"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGU4468623.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8453..9250)
                     /locus_tag="ACHOQQ_RS01585"
                     /old_locus_tag="pgaptmp_000317"
     CDS             complement(8453..9250)
                     /locus_tag="ACHOQQ_RS01585"
                     /old_locus_tag="pgaptmp_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4468624.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9261..10265)
                     /gene="holB"
                     /locus_tag="ACHOQQ_RS01590"
                     /old_locus_tag="pgaptmp_000318"
     CDS             complement(9261..10265)
                     /gene="holB"
                     /locus_tag="ACHOQQ_RS01590"
                     /old_locus_tag="pgaptmp_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGU4468625.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYNTLAPEKGKNTLGIDAVREITEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10262..10903)
                     /gene="tmk"
                     /locus_tag="ACHOQQ_RS01595"
                     /old_locus_tag="pgaptmp_000319"
     CDS             complement(10262..10903)
                     /gene="tmk"
                     /locus_tag="ACHOQQ_RS01595"
                     /old_locus_tag="pgaptmp_000319"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4468626.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRSRYLELAAQDNSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10893..11915)
                     /gene="yceG"
                     /locus_tag="ACHOQQ_RS01600"
                     /old_locus_tag="pgaptmp_000320"
     CDS             complement(10893..11915)
                     /gene="yceG"
                     /locus_tag="ACHOQQ_RS01600"
                     /old_locus_tag="pgaptmp_000320"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGU4468627.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11918..12727)
                     /gene="pabC"
                     /locus_tag="ACHOQQ_RS01605"
                     /old_locus_tag="pgaptmp_000321"
     CDS             complement(11918..12727)
                     /gene="pabC"
                     /locus_tag="ACHOQQ_RS01605"
                     /old_locus_tag="pgaptmp_000321"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGU4468628.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12847..14088)
                     /gene="fabF"
                     /locus_tag="ACHOQQ_RS01610"
                     /old_locus_tag="pgaptmp_000322"
     CDS             complement(12847..14088)
                     /gene="fabF"
                     /locus_tag="ACHOQQ_RS01610"
                     /old_locus_tag="pgaptmp_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGU4468629.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14176..14412)
                     /gene="acpP"
                     /locus_tag="ACHOQQ_RS01615"
                     /old_locus_tag="pgaptmp_000323"
     CDS             complement(14176..14412)
                     /gene="acpP"
                     /locus_tag="ACHOQQ_RS01615"
                     /old_locus_tag="pgaptmp_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4468630.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14623..15357)
                     /gene="fabG"
                     /locus_tag="ACHOQQ_RS01620"
                     /old_locus_tag="pgaptmp_000324"
     CDS             complement(14623..15357)
                     /gene="fabG"
                     /locus_tag="ACHOQQ_RS01620"
                     /old_locus_tag="pgaptmp_000324"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGU4468631.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15370..16299)
                     /gene="fabD"
                     /locus_tag="ACHOQQ_RS01625"
                     /old_locus_tag="pgaptmp_000325"
     CDS             complement(15370..16299)
                     /gene="fabD"
                     /locus_tag="ACHOQQ_RS01625"
                     /old_locus_tag="pgaptmp_000325"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU4468632.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WAMTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16315..17268)
                     /gene="fabH"
                     /locus_tag="ACHOQQ_RS01630"
                     /old_locus_tag="pgaptmp_000326"
     CDS             complement(16315..17268)
                     /gene="fabH"
                     /locus_tag="ACHOQQ_RS01630"
                     /old_locus_tag="pgaptmp_000326"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGU4468633.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17336..18406)
                     /gene="plsX"
                     /locus_tag="ACHOQQ_RS01635"
                     /old_locus_tag="pgaptmp_000327"
     CDS             complement(17336..18406)
                     /gene="plsX"
                     /locus_tag="ACHOQQ_RS01635"
                     /old_locus_tag="pgaptmp_000327"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGU4468634.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18487..18660)
                     /gene="rpmF"
                     /locus_tag="ACHOQQ_RS01640"
                     /old_locus_tag="pgaptmp_000328"
     CDS             complement(18487..18660)
                     /gene="rpmF"
                     /locus_tag="ACHOQQ_RS01640"
                     /old_locus_tag="pgaptmp_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGU4468635.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18712..19233)
                     /gene="yceD"
                     /locus_tag="ACHOQQ_RS01645"
                     /old_locus_tag="pgaptmp_000329"
     CDS             complement(18712..19233)
                     /gene="yceD"
                     /locus_tag="ACHOQQ_RS01645"
                     /old_locus_tag="pgaptmp_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGU4468636.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19432..20016
                     /gene="yceF"
                     /locus_tag="ACHOQQ_RS01650"
                     /old_locus_tag="pgaptmp_000330"
     CDS             19432..20016
                     /gene="yceF"
                     /locus_tag="ACHOQQ_RS01650"
                     /old_locus_tag="pgaptmp_000330"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGU4468637.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20128..21087)
                     /gene="rluC"
                     /locus_tag="ACHOQQ_RS01655"
                     /old_locus_tag="pgaptmp_000331"
     CDS             complement(20128..21087)
                     /gene="rluC"
                     /locus_tag="ACHOQQ_RS01655"
                     /old_locus_tag="pgaptmp_000331"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGU4468638.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            21660..24845
                     /gene="rne"
                     /locus_tag="ACHOQQ_RS01660"
                     /old_locus_tag="pgaptmp_000332"
     CDS             21660..24845
                     /gene="rne"
                     /locus_tag="ACHOQQ_RS01660"
                     /old_locus_tag="pgaptmp_000332"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGU4468639.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQTQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25040..25993)
                     /gene="flgL"
                     /locus_tag="ACHOQQ_RS01665"
                     /old_locus_tag="pgaptmp_000333"
     CDS             complement(25040..25993)
                     /gene="flgL"
                     /locus_tag="ACHOQQ_RS01665"
                     /old_locus_tag="pgaptmp_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU4468640.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPGGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26005..27648)
                     /gene="flgK"
                     /locus_tag="ACHOQQ_RS01670"
                     /old_locus_tag="pgaptmp_000334"
     CDS             complement(26005..27648)
                     /gene="flgK"
                     /locus_tag="ACHOQQ_RS01670"
                     /old_locus_tag="pgaptmp_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU4468641.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27714..28655)
                     /gene="flgJ"
                     /locus_tag="ACHOQQ_RS01675"
                     /old_locus_tag="pgaptmp_000335"
     CDS             complement(27714..28655)
                     /gene="flgJ"
                     /locus_tag="ACHOQQ_RS01675"
                     /old_locus_tag="pgaptmp_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGU4468642.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28655..29752)
                     /gene="flgI"
                     /locus_tag="ACHOQQ_RS01680"
                     /old_locus_tag="pgaptmp_000336"
     CDS             complement(28655..29752)
                     /gene="flgI"
                     /locus_tag="ACHOQQ_RS01680"
                     /old_locus_tag="pgaptmp_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU4468643.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29764..30462)
                     /gene="flgH"
                     /locus_tag="ACHOQQ_RS01685"
                     /old_locus_tag="pgaptmp_000337"
     CDS             complement(29764..30462)
                     /gene="flgH"
                     /locus_tag="ACHOQQ_RS01685"
                     /old_locus_tag="pgaptmp_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU4468644.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30515..31297)
                     /gene="flgG"
                     /locus_tag="ACHOQQ_RS01690"
                     /old_locus_tag="pgaptmp_000338"
     CDS             complement(30515..31297)
                     /gene="flgG"
                     /locus_tag="ACHOQQ_RS01690"
                     /old_locus_tag="pgaptmp_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU4468645.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31469..32224)
                     /gene="flgF"
                     /locus_tag="ACHOQQ_RS01695"
                     /old_locus_tag="pgaptmp_000339"
     CDS             complement(31469..32224)
                     /gene="flgF"
                     /locus_tag="ACHOQQ_RS01695"
                     /old_locus_tag="pgaptmp_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HGU4468646.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGLVLQADPTLRVMSGVLEGSNVNTVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32244..33452)
                     /gene="flgE"
                     /locus_tag="ACHOQQ_RS01700"
                     /old_locus_tag="pgaptmp_000340"
     CDS             complement(32244..33452)
                     /gene="flgE"
                     /locus_tag="ACHOQQ_RS01700"
                     /old_locus_tag="pgaptmp_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU4468647.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLKFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(33477..34172)
                     /gene="flgD"
                     /locus_tag="ACHOQQ_RS01705"
                     /old_locus_tag="pgaptmp_000341"
     CDS             complement(33477..34172)
                     /gene="flgD"
                     /locus_tag="ACHOQQ_RS01705"
                     /old_locus_tag="pgaptmp_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGU4468648.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34184..34588)
                     /gene="flgC"
                     /locus_tag="ACHOQQ_RS01710"
                     /old_locus_tag="pgaptmp_000342"
     CDS             complement(34184..34588)
                     /gene="flgC"
                     /locus_tag="ACHOQQ_RS01710"
                     /old_locus_tag="pgaptmp_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU4468649.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34592..35008)
                     /gene="flgB"
                     /locus_tag="ACHOQQ_RS01715"
                     /old_locus_tag="pgaptmp_000343"
     CDS             complement(34592..35008)
                     /gene="flgB"
                     /locus_tag="ACHOQQ_RS01715"
                     /old_locus_tag="pgaptmp_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGU4468650.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTTPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35163..35822
                     /gene="flgA"
                     /locus_tag="ACHOQQ_RS01720"
                     /old_locus_tag="pgaptmp_000344"
     CDS             35163..35822
                     /gene="flgA"
                     /locus_tag="ACHOQQ_RS01720"
                     /old_locus_tag="pgaptmp_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU4468651.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNASVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35898..36191
                     /gene="flgM"
                     /locus_tag="ACHOQQ_RS01725"
                     /old_locus_tag="pgaptmp_000345"
     CDS             35898..36191
                     /gene="flgM"
                     /locus_tag="ACHOQQ_RS01725"
                     /old_locus_tag="pgaptmp_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU4468652.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36196..36612
                     /gene="flgN"
                     /locus_tag="ACHOQQ_RS01730"
                     /old_locus_tag="pgaptmp_000346"
     CDS             36196..36612
                     /gene="flgN"
                     /locus_tag="ACHOQQ_RS01730"
                     /old_locus_tag="pgaptmp_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGU4468653.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(36652..38187)
                     /gene="murJ"
                     /locus_tag="ACHOQQ_RS01735"
                     /old_locus_tag="pgaptmp_000347"
     CDS             complement(36652..38187)
                     /gene="murJ"
                     /locus_tag="ACHOQQ_RS01735"
                     /old_locus_tag="pgaptmp_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGU4468654.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38297..39220)
                     /locus_tag="ACHOQQ_RS01740"
                     /old_locus_tag="pgaptmp_000348"
     CDS             complement(38297..39220)
                     /locus_tag="ACHOQQ_RS01740"
                     /old_locus_tag="pgaptmp_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4468655.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSAGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39222..39869)
                     /locus_tag="ACHOQQ_RS01745"
                     /old_locus_tag="pgaptmp_000349"
     CDS             complement(39222..39869)
                     /locus_tag="ACHOQQ_RS01745"
                     /old_locus_tag="pgaptmp_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGU4468656.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39880..40464)
                     /gene="rimJ"
                     /locus_tag="ACHOQQ_RS01750"
                     /old_locus_tag="pgaptmp_000350"
     CDS             complement(39880..40464)
                     /gene="rimJ"
                     /locus_tag="ACHOQQ_RS01750"
                     /old_locus_tag="pgaptmp_000350"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4468657.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40700..41908
                     /gene="mdtH"
                     /locus_tag="ACHOQQ_RS01755"
                     /old_locus_tag="pgaptmp_000351"
     CDS             40700..41908
                     /gene="mdtH"
                     /locus_tag="ACHOQQ_RS01755"
                     /old_locus_tag="pgaptmp_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGU4468658.1"
                     /translation="MSRVSQARSLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41972..42619
                     /gene="grxB"
                     /locus_tag="ACHOQQ_RS01760"
                     /old_locus_tag="pgaptmp_000352"
     CDS             41972..42619
                     /gene="grxB"
                     /locus_tag="ACHOQQ_RS01760"
                     /old_locus_tag="pgaptmp_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGU4468659.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAACKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42753..43313
                     /locus_tag="ACHOQQ_RS01765"
                     /old_locus_tag="pgaptmp_000353"
     CDS             42753..43313
                     /locus_tag="ACHOQQ_RS01765"
                     /old_locus_tag="pgaptmp_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4468660.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43419..44465
                     /gene="pyrC"
                     /locus_tag="ACHOQQ_RS01770"
                     /old_locus_tag="pgaptmp_000354"
     CDS             43419..44465
                     /gene="pyrC"
                     /locus_tag="ACHOQQ_RS01770"
                     /old_locus_tag="pgaptmp_000354"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGU4468661.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESTCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        10722 a        12057 c        11080 g        10614 t
ORIGIN      
        1 acaggttaca gcaatgaaaa tgttcttcgc tattgagaat aacatgaatg gtgcattggt
       61 cataccaaaa ttgatgttaa tcaagttttg ttgctgaaaa gataggcgac tttaccgggt
      121 gagatcaaaa aacagagttt attgtttatt atttgagcga atctggaagg gtgatatagg
      181 gtgaagcgta tatcgagggc gtttggtcgt actgataaga tgcgcagcat cgcatcaggc
      241 attgatgctc gactgtcgga tgcggcgtga acgccttatc cgacttacag aatccggagc
      301 gcatcaggta ccggcaaggt tacctgatgc gacgagggca tttacccgag ggttttgaac
      361 gctttaatgc gctgcaaatg cggagagata ttcttgaatt tgtgcgtctg ctcttcgtcc
      421 cagacaatct cgtaataatg atgcaactct tcagatgtcc gctggctgtt aagcgcttca
      481 tcattacgcg acaaaatcac cagacaacga tcgcgattct tctcgcggaa gttggtcaca
      541 cacttagtgg caatatcggc atactcttcc gggcgatcaa tcttgccttc catgttctca
      601 taagggaaca aattagggtt gaagatcacc tggcggatgt cgcaaagaaa accaatccgt
      661 tccgcccagt atccgcccaa gccaacgccg caaattagcg gacgctcgtc aacgttcagt
      721 tgcaacattt tgtccacttc tttaagcaga tgctgcatat catgtttcgg atgccgcgta
      781 ctgtagctta tcaagcgtac atccgggtca ataaactgca attgtaagac tttctcgtgg
      841 ttacccggac tgttagagtc aaaaccgtgt aaatagataa tcatcgcttc ctcacataag
      901 ccagggttag tgacctgctt tctcttcctg ccagcgttca tgtaactgat tcagacgggc
      961 gctgatcgct ttccagcgag ccgagtccat cagttcctgt cgcgaaaatg aacctttatg
     1021 atacaaacgt gtaacacgtt ctgccttgat cggtgacaga ttatctaaca cgctgacggc
     1081 ccctttacga ttattgcaga ccaggatcat atcgcaaccc gcatccagtg atgcctgccc
     1141 gcgttcggca taactgccca taatcgcggc accttccatc gataaatcgt cagagaaaat
     1201 cacgccgtca aaacccagtt cctgacgcaa aacggttttc agccagtagg gagaaccgct
     1261 cgccggacgc ggatcaacat cactgtagat cacatgcgca ggcataatgg cgtcgagttt
     1321 gttttcgcgg attaaggaac tgaagaccga catatcttta gcgcgaatct ccgcttgtgg
     1381 acgtggatcg cacggtgttt ctttgtgtga gtctgccgtt actgcaccgt gtccagggaa
     1441 gtgtttcccg gtcgttttca ttccggcttc atgcatacca tcaataaacc ggctggcaat
     1501 tgccagggct ttttgtggat cggcatgata agaacgctcg ccaatcgccg cgctgatatg
     1561 cccgacatcc agcacaggcg caaagctgat atcaatatcc atagcgatca tttcgctggc
     1621 catcaaccaa cctgcctcct gcgccagttt gccaccctct tccattcctg acagcgcagc
     1681 gaatgattgc gccgctggca agcgggtaaa accttcacga aaacgctgca cgcgtccacc
     1741 ttcctgatca accgccacca ccagatgatt gcgcgaagct gcgcggatct ggcgcaccag
     1801 ttcacgtaac tgggcaggat catgatagtt acgcgtaaag agaatcagcc ctcccaccag
     1861 cggatgcgcc agtatttcac gctcttccgc gtccagttcg taaccttcga catccaacat
     1921 tactggaccc acactgctct ccttattgtt ttattaatag ctgccgccag gtgtcatcgg
     1981 ccagcctgat aaattgttga tcgccggttt gtcgccagcg gtactcaaac caccctgctt
     2041 tgagcatcag cagccaggga aaccatcgcc tgacctgacg ccataattgc gccggataaa
     2101 tcttcgcgcg agtggcatag tcattgacca attgtcggtg ctgttcagca ttttccaccc
     2161 acaccgccgc cagttccagc gcgatatcac catctcctgc atactcccag tcgatgagtt
     2221 ttaaccctga cgcgctatgc actaaatttc cggcgtggac atccatatgc aatggactta
     2281 agcgtaaagg ccgtggttcc cgcgctttgc gcagacgttt taacattcgc aaccaaccca
     2341 ctgtccgccg cgccggatcg ctttgctgcc agtacagttc cagtaacggc aacaacgtta
     2401 ttcgccagcc aaaacgtggt tgttgatgta gataatacag caagcctgcc agttcgttgg
     2461 tatccggcaa atacgttttt accgcgccgg gcagatagtc gactaccatc cagtcatgga
     2521 gatataaatg cggcttcggt gcaatgcatg cgggtagttg tgataaagcc cgatactggc
     2581 gcaagaacgc tgactgcggc gcatcaggat cgtgcggctg acgcacaaca aaacgctggc
     2641 cctggtgttc aatgagaaaa ctcccgccac taagcccact attaaacgtc gtgacgggat
     2701 gaaactgcgg gaaaaagcgc gacagcaatt cgtcgcgcgt gatgggatta ttgctgcgaa
     2761 acggcacctt tacctgacca gataatttcg cccgtctgca ccagcatcag ctgcatttgt
     2821 agggtcggag cgttaacgtt gccagaggcg ctggagtaca gcacgtaatg agcgccgaca
     2881 ttgcgggcaa tgcctatggc tttactacgg gtgcctaaac tgtcctgcgg cgacaaacct
     2941 aactgttgct tcgccatcga cagctgctgg gcggaaacca gggtaaattt cccgttatta
     3001 gccagcgcat ttcgcagcgt ttcggtcgct tctgcggcat tcagcgaacc gttagtacgg
     3061 ttgttaacgc tatcaaccag caggacgcta cccgcagtca ccccgtcagc cccaagcatc
     3121 ttactgacca tcggctgcat tgcgccattc cagtcataat ggcgaatatg cggcgcaggc
     3181 ggtgccgttt gatcttcgtg ctcaattggg cctggctgct gcgggatcgt cggcaccgag
     3241 ggcactgtgg ggacaggctg ttgtggctcg gctggttgtt ccggcgctgg tttcacttct
     3301 tctaccggtg caggttcacg ttgccccaca cacccggcga gaaacatcgc cagcgcggta
     3361 atcaaggcgt agcgactcat ttttgtcatc aagattcacc ccttacaaat atagataaag
     3421 tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc
     3481 gggaatggta acgctgcgtg gcctttccag aggatgcatc tccagccctc tggcgtcata
     3541 ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa gcgttgagga
     3601 tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt tctgcactga tgcccgcagc
     3661 caataaagat gactccatca ccagcgattg ctcatcattt accggaattt ccggatgtga
     3721 acgacagccc accagcagta acaacgccag agacaccagc ccaaagcatc cttttctcat
     3781 cgttacagac ctttatgcgc cagcattggt cccagcggac ggccacccaa caagtgcata
     3841 tggatgtggt aaacctcttg tccgccatgg cggttggtat tcatgatcag acgataacca
     3901 tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat acgtcccagc
     3961 gcctgctcat gctcagctga cacgtcgttc acagtcggaa tgaggatatt cggaatgatc
     4021 agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg
     4081 tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt ttcttctgcc
     4141 acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc gccacactgc
     4201 gagtatagag tatggtcgag ttaaacctgc tctttcaacc ttaactcacg atttcttaag
     4261 caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc tgcgaatgcg
     4321 ctgaaaagac attctctgtt tggtttacac gtcaaatatg aatgcgtata tttctcattt
     4381 gcatttacat acaaaattat tcgcacacta aatataaccg ctcatcaccc tgctccggca
     4441 tgagcctgac gacaacataa accaagagat ttcaaatgct ttcaacacaa tttaacaggg
     4501 ataatcaaca tcaagccatc accaaaccgt cactaattgc cggttgcata gcactggcac
     4561 tattaccttc tgccgctttt gctgcaccag ccactgaaga aacggtgatt gttgagggtt
     4621 cagccacagc tccagatgat ggcgaaaatg attacagcgt aacgtctacc tctgcgggta
     4681 ccaaaatgca gatgactcaa cgtgatattc ctcagtcggt cactattgtt agccagcagc
     4741 ggatggaaga tcagcagcta caaacgctgg gcgaagtgat ggaaaacacg ctggggatca
     4801 gcaaaagtca ggcggattcc gatcgtgctc tttattattc ccgcggattc cagatcgata
     4861 actatatggt tgatggtatc cctacctatt ttgaatcgcg ctggaatctg ggcgacgcac
     4921 tttctgatat ggcacttttt gaacgcgtag aagtagtgcg tggcgcgaca ggactcatga
     4981 ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg accagtcgtg
     5041 aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg tatgtggcgg
     5101 atttacaaag cccactcacc gaagacggta aaatccgcgc gcgaattgtc ggcggctacc
     5161 agaataacga ctcatggctg gaccgctaca atagtgaaaa gaccttcttc tccggcattg
     5221 tcgatgctga tttaggcgat cttacgacgc tttcagccgg ttacgaatat cagcgcattg
     5281 atgttaatag cccaacctgg ggcggtttac cgcgctggaa tactgatggc agcagcaaca
     5341 gttacgatcg tgcacgcagt accgcacctg actgggcgta caacgataaa gagatcaaca
     5401 aggtctttat gaccctgaag cagcggtttg ctgatacctg gcaaacgacg ctgaatgcca
     5461 cccactccga agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat ataaacaaag
     5521 cggatggtat gctggttggg ccatacagta attatggacc tggttttgat tatgtcggcg
     5581 gcaccggttg gaacagcggc aaacgtaaag ttgatgcgct ggatttgttc gctgacggtc
     5641 gttatgaatt gtttggtcgt cagcacaatc taatgtttgg tggcagttac agcaaacaaa
     5701 acaatcgtta cttcagttca tgggccaata ttttcccgga tgaaattggc agtttctaca
     5761 actttaatgg caatttccca caaaccgact ggtcaccaca gagcctggcg caggacgata
     5821 ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc
     5881 tgatcctcgg cgcacgttat accaactggc gggttgatac gctgacttac agcatggaga
     5941 aaaaccacac cacgccttat gctggtctag tgtttgacat caatgacaac tggtcgacct
     6001 acgccagcta tacctctatt ttccagccgc aaaatgatcg tgacagttca ggcaaatatc
     6061 tggctccaat caccggtaac aactacgagc tgggtctgaa atcggactgg atgaatagcc
     6121 gtctgaccac cacgttagcc atcttccgta ttgagcagga taatgtcgct cagtccaccg
     6181 gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat gggacagtca
     6241 gtaaaggggt ggaatttgaa ctcaacggcg caattaccga caactggcag ctgacatttg
     6301 gcgcaacgcg ctatattgca gaggataacg aaggaaacgc cgttaatcct aatctgccac
     6361 gcaccacggt taaaatgttc accagctatc ggttgcctgt catgccagag ttgacagtcg
     6421 gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacaccg tatggcacct
     6481 tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac caggtgacga
     6541 aaaacttctc gttacagggg aacgtcaata acctgttcga caaaacctac gataccaacg
     6601 tggaaggttc tatcgtctac ggcacaccgc gtaatttcag cattaccggc acgtatcaat
     6661 tctgaataac acctgtaaaa aaggcagcca tctggctgcc ttagtctccc caacgtctta
     6721 cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg gatttagtac
     6781 cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc agtttcttca
     6841 ggccggcctg atccacttta gacacatcag caacgctgac gcgcagacgg gtaatacatg
     6901 cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc ggtgccattt
     6961 cgctggtacc tgtcgctttt gcatcttcag tcgcgtcttc acgacccggc gttttcagat
     7021 ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac aatcgcataa ccgataccga
     7081 cgatcgggaa cagccacagt ttgctgctgt taccagacag aacgatgaag tcgatcagac
     7141 cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat acagattggg aatgccaggc
     7201 ctgccagaat cgcgtggatg atgtacagga tcggcgcaac gaacatgaag gagaactcga
     7261 tcggctcggt gataccggtc aggaacgagg tcagcgccgc ggagatcata ataccgccca
     7321 ctttcgcgcg gttttctggt ttagcagagt gccagatagc aattgcggca gctggcagac
     7381 cgtacatttt gaacaggaag ccaccagaca gtttacccgc agtcgggtca cccgccatat
     7441 aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca
     7501 tctggaaagg tacgttccag atgtggtgca gaccaaacgg taccaggcaa cgttcgatga
     7561 aaccgtaaat gccaaacgca actaccgggt tctggtaagc agcccactga gagaaggtct
     7621 ggattgcaga accaatcggc ggccaaatga aggacagcac aacgccagta aagatggcag
     7681 ccaggccaga aatgatcggc acaaagcgtt taccggcaaa gaagccaaga tactcaggca
     7741 gcttaatacg gtagaaacgg ttaaacatgt acgctgcgat cgcaccggag ataatccctc
     7801 cgagtacgcc agtatccgcc aggtgtttag aggcgatttc ttcagcaggt aaatgcagta
     7861 ccagtggcgc aaccacggcc atggttttaa ccatgatgcc ataggcaaca actgcggcca
     7921 gcgcggatac gccatcgtta ttggtaaagc cgagggcgac accgatcgca aaaatcagtg
     7981 gcatgtttgc aaagacggaa ccgcctgctt ctgccataac atgcgataca acggcgggca
     8041 gccagctgaa attcgcggaa ccgacgccca gcagaatacc tgcgataggc agtacggata
     8101 ccggcagcat cagcgattta ccgacctttt gcaggttagc aaatgcattc ttaaacataa
     8161 ttgagagtgc tcctgagtat gggtgctttt tttacgttct cacgcgtggc aaggggggag
     8221 agcctcgccg tgtacagggc atctaagcgc cctttattta ttacacagag taaaataatt
     8281 cagtgccaat ttgtttgacg gctatcacgt ttcaacttca gatcattgga gaagttgcac
     8341 attttgacaa aacatatgtc acaatatttc aaacgtggtt attcaccgcc caattttcgg
     8401 cggtgaactt tactcgtttt agccattaat tacgagcttt aaaaaaactc attcaacgga
     8461 tggattgaag gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg gtggttacct
     8521 gcgccagttc ttcaacggca acacctttca acacagccat gtattctgca acgtcacgaa
     8581 ccatcgcagg ttgattctct tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag
     8641 tttccaccag taaccgatcc agggggacat aacgcgcagc atcgcgcagt tgctccgcat
     8701 tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt aatttacccg
     8761 ccgtttctct gtcctctgta aaacagtgta gtacgccacc gcaatccgtc actttttctt
     8821 cgcgcagaat tgccagcgta tcggcacggg cgtcacgggt atggacgata accggcttgt
     8881 tcagttcacg accaatctgg atatgatgga tgaaggactc ttgctgacgt actttagttt
     8941 ccggtgtgta ataataatcc agcccggttt cacccagcgc tacaacaccc tcttctgccg
     9001 ccagacggcg taaatcttct acatcgtagg gatcattctg gttgagtgga tgcacgccac
     9061 aagaaaatac gacgttgtca cgttcgccta ccagatcccg catatgtaaa taacccggta
     9121 atgttgtggc gactgccaga caaaatttca catcgcgtgc ggcggctttc gccagaacgt
     9181 catccacgtc cttatgcaaa gattcataat ccagaccatc gagatggcag tgtgagtcga
     9241 ctaaaaacat gatgtctctc ttaaagatga ggaaccggta gcacaacgcc cggttgcagg
     9301 taatgctcaa tacgcagtaa aagatcggtg atgagaagct cgcggttgat gcctgtgaca
     9361 gacattaact gttcacgaat gtggcaaaca tcccccagta tagcctgcag gcgcgaggga
     9421 gaaagatggt ttgccagttc gacgaccagg cccggcacat caacattggt cacctgcgca
     9481 gcgccatgat ggcgttttag cgcatccatc agcaacgttg ccagccagtg taaacgcgcc
     9541 ggagcttgtt catgattaag ggccgctagc agcgaatacc agtcgcccga tggcacgcta
     9601 tatgccaacg cctgacacaa tgtttcacga gcctgccagt tatctccctg aaacaacgcc
     9661 agtgccgcgc caggcgaacc ggcgcttaaa cgcaatgcgg caagtaatgc atcctgtgac
     9721 attgtcactt cgcgtgaaag ccaggtcacg gcgtactgtt ccggcggcgg cgcaaggtaa
     9781 tgtaaccgac aacgactacg taatgttgcc agtaaacgtt caggctcgcg ggtagccagg
     9841 aaaaaccagg tttctgctgg tggctcttca agcgttttca gcaatgcgtt agccgcggcg
     9901 tcggtcagta aggcagcatc ggttacccag acaactttcg caccacctaa gcgtgcgtgc
     9961 tcattcagct tttcggtgat ctcacgtacc gcatcaatgc ccagcgtatt tttccctttt
    10021 tcgggagcca gggtgttgta atcgggatgc gtgccagcct gcatcaactg acatccacga
    10081 cagtgaccgc aacttttgtg gccctgcggt tgttggcaga gtaaataacg gctcagggcg
    10141 tagattaaag catcatcgcc catgcccggt aacgcctgaa tgagtagcgc atggtgacct
    10201 cttccggcct gatagctggc taccagtttt tcgaaatcag gtcgtaacca tggataccat
    10261 ctcatgcgtc caactccttc acccagtggg tcacggtagt gcggattgca tccatcacgg
    10321 cctccagcgg ctgggtggca tcaatggtat gaatactgtt atcttgtgca gccagttcca
    10381 gatagcggga gcgggtgcga ttaaagaaat cgaaagactc ttgctcaata cgatccagct
    10441 cgccgcgcgc acgcgcgcgt tttaagccaa cttccggggt aacatcgaga tagagcgtta
    10501 agtcggggcg aaaatccccg agaacagcat cacgcagtgt tgccagcata tgttggtcaa
    10561 taccacgtcc gccgccctga tacgcctgag tggagagatc gtggcgatcg ccaatcaccc
    10621 aggtgccgtt agccagcgca ggtttgatga ccgtttctac cagttgaacg cgcgcggcat
    10681 aaaacatcag aacttcggct ttatcggtaa tgacttcatc gcctaccgat ttgatatcca
    10741 gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc aggttcccgc gtgaaaacca
    10801 tgtcgcggat acccagttgc tcaagcgtct caaccaccac attacgcgcg gtagttttgc
    10861 ctgcgccttc cagcccctca atgacgatat acttactgcg catttttttc cttaagcact
    10921 ttcagataat cctgcacaga cttgttatga cttgcaagat tggtattaaa cgtgtgacca
    10981 cctttaccat cggccacaaa atagagatac ggcgtttttg ccggatgcgc agcagccttc
    11041 agcgaatccg cccccggcgt agctatcgca cccggcggca aaccggtaat ggtataggtg
    11101 ttatacgctg tcggcgtttc caggtctgca cgagaaagtt tgccattata acgctctccc
    11161 atcccgtaaa tcacggttgg gtcggtctgc aggcgcatac caatgcgtaa acggttgata
    11221 aataccgagg caacctgatc gcgttcactg gcaacggcgg tttctttttc gataattgat
    11281 gccatcgtca ccagctgatt tttatcttta taaggcagac cgtccgcacg cccttcccag
    11341 gcgctatcga ccgctttcac cattttcttg tgcgctcgct tgagtaacgc gacatcggtg
    11401 gtattggcgg tatacatcca ggtgtctggc cagaaccaac cttcaatcca ctccgggttt
    11461 tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt cactcagcgt gtgcttgata
    11521 tacggagcct cgcgcaattg cttgaggtag tcgctcagac gcatcccttc taccagtcgc
    11581 agcgggaact gtgcttcttt accgctttcc aacaatttca gcatctcgcg cacggtcatc
    11641 tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa gatccggttc gatacgcagc
    11701 aaccattgaa aaacccgtgg gcgattgatg atcttatcgg cataaagctg ttcaccaagc
    11761 gccagacgtc cggtccctgg cttcagggta aatatcgtct cttctttgat aagcaatttg
    11821 ctgtcggcaa gatggcgaac cttccagacg cccacaccag cggcgatacc cagtaccacc
    11881 agcaataaca agataatcaa taacactttt ttcatgacta attcgggcgc tcacaaagtg
    11941 gggctaaata ttcatataac gttgctgacg aaaaggagac atcgccacag gcacgtacgg
    12001 gcatcactgg cattaacgca ttacaaataa ccatctcatc tgcctgcaac gcctcttcca
    12061 gagaggcttg cacttcgaca agctgataag aggattgtgc cagtaaacgg atacagaatt
    12121 gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg cgtataaact acgttgccct
    12181 tccgccagaa caaattagcc gcacagcatt ccgtaaccca cccttcgctg tcaaggacca
    12241 gcgcctcatc agcgtttgtc tgctcaagat gagagcgaat caatacttgc tcaagacgat
    12301 tgagatgttt aatacctgca agatgaggat tgcgccccag ccgcaccggg cttagcgcca
    12361 acgtcatccc ctcgttacgc aaacggtcgt aatgtgcagg ataagccgta acggagagaa
    12421 tccgcgttgc cggtccactg ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga
    12481 tcacgacttt cagtacacca ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa
    12541 gctgtggcca gaagtcacag gaaatcatca accgctgaca agcatcctgt agtcgctgga
    12601 tatgcgccga taacaaactg actttaccgt cgataactct ggcggtggta aaacaaccat
    12661 caccaaactg cgttgcccga tcgcttactg ccagcgattc ctgcttataa ccgttaatta
    12721 agaacatacc ggctccttat cagagaattg ttagtgtggc agcatgttca ctacggaaca
    12781 agtcggaata aaagctaaga aaaaaggccc gcaagcggac cttttatatg ggtggaaaat
    12841 gacaacttag atctttttaa agatcaaaga accattagtg ccaccgaagc cgaaggagtt
    12901 acacagcgtg tattccattc cgctgacctg acgtgcttca tgcggtacga aatccagatc
    12961 gcaaccttca tccgggttat ccaggttgat ggttggcgga acagcctgat cgcgcagcgc
    13021 caggatggag tagatagatt ctactgcacc cgccgcacct aacaggtgac cggtcataga
    13081 tttcgtggag cttaccaaca cacggcttgc agcttcaccg aagatggttt tcaccgcctg
    13141 cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg
    13201 actcgcttca atgcctgcat cacgcagagc atttgccatt gccagagctg cgcctgcgcc
    13261 attttctggc ggtgacgtca tatgataagc atcgctgctc ataccaaagc cgacgagttc
    13321 agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta ccagcatacc
    13381 ggcaccatcg cccagtacga agccatcacg ctctttatcc cacgggcggc tcgccgcttg
    13441 cgggttatca ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg
    13501 cgtactggct ttctctgcgc cacctgcaac catcacgtca gcatcgccat acgcgataat
    13561 acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct
    13621 tgggccacgc aggccataca tgatagtcag atgacctgcc accatgttca caatcgttga
    13681 cggaacgaag aatgggctga tcttacgtgg accaccgttc atcagagatg tgtggttttc
    13741 ttcgatcagt ccgaggccgc caatcccgga gccaattgcg gcaccaatgc gggttgcgtt
    13801 ctcttccgtt atttcaaggc cagaatcctg catggcctga acgccagcga caattccata
    13861 ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa
    13921 atcctttact aagccagcaa atttcgttgc ataggcgcta gtatcgaaat ggtcgattag
    13981 gctgatgcca ctctgaccgg caagcagagc tttccaggta gactctacgg tattgccgac
    14041 aggagacaac atgcccagtc cggtcacaac tacacgacgc ttagacacgt ttgtcctcca
    14101 gggagggaaa aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg
    14161 cctggagatg ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt
    14221 ggtgattttc tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat
    14281 taccagctca acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt
    14341 ggtaacttct tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc
    14401 gatagtgctc atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt
    14461 gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt
    14521 attttgaggc cttacccccc aataacgcaa atcattttgc actaattatt actcataacc
    14581 acgcagactg cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc
    14641 gccgttcaca tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc
    14701 aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat
    14761 acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc
    14821 aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc
    14881 gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac
    14941 cacagaaccg atagtgataa tacgaccatg acgctttttc atcatagcgc gcattaccgc
    15001 ttttgacaga cggaaaacag atgaaaggtt ggtttcgata atatcgttcc actcttcatc
    15061 tttcattcgc attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac
    15121 ttcaccaaat tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac
    15181 attcaacatc agacctttgc cgttagcacc taaataatca ctgatcgcct gagcgccatt
    15241 ttcactggtc gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc
    15301 gcggccaatt ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcataat
    15361 tttcctcttt taaagctcga gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc
    15421 ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc actttgcccg ggccgacttc
    15481 atagagatgt tctacgcctt gcgctgccat gtactcaaca gacttcgtcc actgaaccgg
    15541 gttatacaac tgacgtacca gtgcgtcacg gatggcatca ccattggttt cgcatttcac
    15601 atcaacgtta ttcacaacag gaactgttgg tgcgttaaag gtgattttcg ctaattctac
    15661 tgccagtttg tcggctgctg gtttcatcag cgcacagtga gatggtacgc tcactggtaa
    15721 cggcagagcg cgttttgcgc ccgcagcttt acaggcagcg ccagcacgct caaccgcttc
    15781 tttatgaccg gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg
    15841 accttctgca gcttcttcac acgctttcgc aatagacgca tcatccagac cgatgattgc
    15901 cgccatagcg cccgttcctt ccggtacggc ttcttgcatg aacttgccgc gcatctcaac
    15961 cagacgcacc gcatcagcga aatcaatcac accagcgcaa accagcgcgg agtattcccc
    16021 caggctgtga ccggccatca ttgccggtgc tttaccgccc tgctgctgcc atacgcggta
    16081 cagcgcaaca gaggcagtca acagcgctgg ctgggtttgc caggttttat tcagttcttc
    16141 agctggcccc tgctgggtca tcgcccacag gtcgtagccc agcgccgcag aagcttctgc
    16201 aaacgtttct tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg
    16261 agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa
    16321 acgaaccagc gcggagcccc aggtgaatcc accgccaaag gcttcaagca gaaccaactg
    16381 ccccggctta atgcgcccgt cgcgtacagc ttcatccagc gcgcacggga cagaggccgc
    16441 agaggtatta ccgtggcgat ccagcgtcac cacgacattg tccatcgaca taccgagttt
    16501 tttcgccgtt gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg
    16561 agaacggtca agattattcg ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac
    16621 cgcaaccttg aagacttcgt tgcccgccat cgtcagatga attgaattct ctggattcac
    16681 gcggtcggcg tttggcagcg tcagcaattc accataacta ccgtcggcat gcagatgggt
    16741 ggagatgatt cccggctctt cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa
    16801 aataataata gtcccacgat ctgttggatc gcaggtgcgc gccagtacat cggaaccgac
    16861 gaccagagca tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata
    16921 ggtgaaacct gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa tgcccaacat
    16981 gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac
    17041 gatcaggcca atctggtctt tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc
    17101 aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt
    17161 acgagtgaca atccactcgt cagaggtgtc caccattttt tccaaatcgg cgtttgtccg
    17221 cacttgttcg ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc
    17281 acttttcggt tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc
    17341 gcagagttcc gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca
    17401 ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg
    17461 cgaccgcaaa agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc
    17521 ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc
    17581 tcttttgtag ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct
    17641 gagatttcag cagagaaagg aacatcctga caacaccttc catcgtcttt aatgtgacat
    17701 ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt
    17761 caagatagcc gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa
    17821 tactgtcgag accctttact tcttcttcac caatattgag caacgccacg cgaggattgg
    17881 gaatttccac cacctcttca gccagaactg agcccataat ggcaaattgc accaacattg
    17941 tgctgtcaca atcgacgttg gcccctaaat cgaggaccac cgttttgccc ttttgctgat
    18001 gcggtaatac cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt
    18061 ttgccagccc catcagtgcc ccggtattac cggcactgac acaggcttgc gctcgacctt
    18121 ctttcaccag ctccagggcc acgcgcattg aactcccacg actggcgcgg atagcttgcg
    18181 aaggccgggc atcactggcg ataactgact gcgcaggaat aatctgcaga cgcgaacgtt
    18241 gttcaaagtc agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa
    18301 gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc
    18361 caaaatcccc tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc
    18421 ccggtttggt ctttccccag ttgcctgggg aaaatcctca ctaagcttca tcacgcagac
    18481 gcgtgattac ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg
    18541 acgcaggtgt ttttcaccag aagttttgtc tacagacagg ctggtgactg cggtcagcgc
    18601 gtcatgggaa cgacgcatgc cacgtttgga acgggttggt ttattctgtt gtacggccat
    18661 ggaccttact cctcaattac ggtttatccc cgatccaacg gggagcacca attacttacg
    18721 ctttaagctg gctaatacgg caaatgggtt tggcttttgc gcttcttcag gcagttcacc
    18781 aaagaccatg tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg gaactaccgg
    18841 caaggcgagg atgatttcat cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt
    18901 aacctcaatc ggttcatacg cttccggcag tgcttcagcc tgttcgtctg aacgcacagg
    18961 actaaaacaa tacgttgtgt agacctgatg agtaaacggc ttcccgcaac gctgacactc
    19021 gagcgttacc gtcaccttcg catcgccgtt taacactgcg agacgttggt tatcgatagc
    19081 gaacgacatg gagcattcca catcactgtc cacactgact acggattcgg cgacgcgctc
    19141 aacctgatca ggggtataga taccctggta atcaaggcgt ttttgagccg tacgaaccgg
    19201 atcgagagtc aggggtaatt ttaccttttg catagggcgc gcatattaac tttgtaacgt
    19261 catagagtca aagaaaaagg cagcctgtgg ttgccttttg ccaataattc gcacacattg
    19321 cgggttactg ctttattttc gttcagtgct gataacgaac atcaacctgc aaaacgaagc
    19381 gcatagttta aaatggctat catcaatacg ctatatctga tggaaaaaaa tatgcctaaa
    19441 cttattttag cctccacctc gccctggcgt cgcgctcttc tggaaaaact gcaaatctct
    19501 tttgaatgtg cagcacctga ggtcgacgaa accccacgca gcgacgaatc accgcgacag
    19561 ttggtgcttc gactggcaca agaaaaagcg caatctctgg cgtcacgtta tccggatcat
    19621 ttaattattg gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta
    19681 acggaagaaa atgcccgtct gcaattacgc aaagccagcg gcaatatcgt caccttctat
    19741 actggactgg cgttgtttaa ttcggcgaat gggcatctgc aaacagaagt ggagcctttt
    19801 gacgtccatt tccgtcacct gagcgaggcg gagattgata attacgtgcg taaggagcat
    19861 cccctgcact gcgcgggtag ctttaagagt gaaggatttg gcattacgct gtttgagcgt
    19921 ttagaggggc gtgaccctaa cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg
    19981 ttacgtcggg aagggaaaaa tccgctgatg ggatagtttt ttgcgccaga tgcggttacg
    20041 tcgcatccgg caatcggtgc tggcggcctg atgcggcgta aacgccttat caggccaacc
    20101 gcccattatc aggcttatat gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg
    20161 cttcaaacca tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt
    20221 aaacttcaac gctgcggcgt gcaggaacag acgatttaat cccgtgcctg cttcagtgag
    20281 ctgtctgtca aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacccgcata
    20341 ctgtgtatgc acacggatct gatgagtacg tccggttacc gggctacagc gcaccagggt
    20401 ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact
    20461 cacacgcacg atacgttcgc cgctttgcag aatatttttc agtaacggcg cttgaacgct
    20521 cttcacatgc gactgccact gaccgcgcac cagcgccagg taatcttttt gcatcccttt
    20581 ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaatac
    20641 acctgaagtg tcccggtcga gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa
    20701 cgcccgcaaa ccttcgataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc
    20761 ggaaggttta ttcagcacca ggatatgatc atcttcatat aagatgacgt ccgccagcgc
    20821 cgccaccttt tgcagatgtg gtgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg
    20881 tgggatacgc acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt
    20941 cacccgcact tcgcctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa
    21001 ttgggtacgc aaaaagttat cgatacgttg ccccgcttcg tcagcggtga tagcaacaat
    21061 ttttacggat ggagtctctg ttttcatggt tggcgattct aattagccaa caggattcgc
    21121 gccactcatt tttctatgct tatatttact tttgcacctt attacttcac tgcgtgctca
    21181 ctttattgat ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg
    21241 gtgataaatg gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt
    21301 ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg
    21361 atcatccacg cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg
    21421 gttgcagtcc ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt
    21481 tgataattgc gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga
    21541 gctgacgata accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg
    21601 gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga
    21661 tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg
    21721 ggcagcgtct gtatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca
    21781 tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg
    21841 gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta
    21901 actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaggtca
    21961 ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca
    22021 gtctggcggg tagctatctg gttctgatgc cgaacaaccc gcgcgcgggt ggcatttctc
    22081 gccgtatcga aggcgacgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc
    22141 cggaaggcat ggggcttatc gtgcgcaccg ctggcgtcgg caaatctgct gaggcgctgc
    22201 aatgggattt aagcttccgt ctgaaacact gggaagccat caaaaaagcc gctgaaagcc
    22261 gcccggcccc gttcctgatt catcaggaga gcaacgtaat cgttcgcgca ttccgcgatt
    22321 acttacgtca ggacatcggc gaaatcctta tcgataaccc gaaagtgctc gaactggcac
    22381 gtcagcatat cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg
    22441 gcgagatccc gctgttcagc cactaccaga tcgagtcgca gatcgagtcc gccttccagc
    22501 gtgaagtccg tctgccatcc ggtggttcga ttgttatcga cagcaccgaa gcgttaacag
    22561 ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa actgcgttta
    22621 acaccaacct cgaagccgcc gatgagattg ctcgtcagct gcgcctgcgt gacctaggcg
    22681 gcctgattgt tatcgacttc atcgacatga cgcctgtacg ccaccagcgt gcggtagaaa
    22741 accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc
    22801 gctttggcct gctggaaatg tcccgtcagc gcctgagccc atcactgggt gaatccagtc
    22861 atcacgtttg tccgcgttgt tctggtactg gtaccgtgcg tgacaacgaa tcgctgtcgc
    22921 tctctattct gcgtctgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg
    22981 ccattgttcc tgtgccaatc gcttcttacc tgctgaatga aaaacgttct gcggtaaatg
    23041 ccattgaaac tcgtcaggac ggtgtgcgct gtgtaattgt gccaaacgat cagatggaaa
    23101 ccccgcacta ccacgtgctg cgcgtgcgta aaggggaaga aaccccaacc ttaagctaca
    23161 tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac
    23221 gtaagcgtcc ggaacaacct gcgctggcaa cctttgccat gccggatgtg ccgccagcgc
    23281 caacgccagc tgaacctgcc gcgcctgttg tagccccagc accgaaagct gcaccggcaa
    23341 caccagcaac tcctgcacaa cctgggctgt tgagccgctt cttcggcgca ctgaaagcgc
    23401 tgttcagcgg tggtgaagaa accaaaccga ccgagcaacc agcaccgaaa gcagaagcga
    23461 aaccggaacg tcaacaggat cgtcgcaagc ctcgtcagaa caaccgccgt gaccgtaatg
    23521 agcgccgcga cacccgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc
    23581 gtaatcgtcg ccaggcacag cagcagactg ccgagacgcg tgagagccgt cagcaggctg
    23641 aggtaacgga aaaagcacgt accaccgacg agcaacaagc gccgcgtcgt gaacgtagcc
    23701 gccgccgtaa cgatgataaa cgtcagacgc aacaagaagc gaaggcgctg aatgttgaag
    23761 agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta
    23821 aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag
    23881 ttgcaccggt ggttgaagaa actgccgctg ccgaaccaat tgttcaggaa gcgccagctc
    23941 cacgcacaga actggtgaaa gtcccgctac cagtcgtagc gcaaactgca ccagaacagc
    24001 aagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct
    24061 cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc
    24121 caacccaatc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca
    24181 aagtctggat ccgctatccg attgtacgtc cacaagatgt acaggttgaa gagcagcgcg
    24241 aacaggaaga agtgcaagtg cagccgatgg tgactgaggt gcctgtcgcc gccgctgtcg
    24301 aaccggttgt tagcgcgcca gttgttgaag aagtggctga agtcgtagaa gcccccgttc
    24361 agattgccga accgcaaccg gaagtggttg aaacgacgca tcctgaagta attgccgccg
    24421 cggtaactga acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagtcg
    24481 cagaacacgc agaaccggtg gttgaaccgc aggaagagac ggcagatatt gaagaagttg
    24541 ccgaaactgc tgaggttgta gttgctgaac ctgaagttgt tgctcaacct gccgcgccag
    24601 tcgtcgctga agtcgcagca gaagttgaaa cggtaaccgc ggttaaacca gagatcaccg
    24661 ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg
    24721 aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg
    24781 caggtggtca tacggcaaca catcatgcct ctgccgctcc tgcgcgtccg caacctgttg
    24841 agtaataatt agctcaagta atcaagccct ggtaactgcc agggcttttt tatttcatct
    24901 ttgaatcaac atattaaaat tcatcgaatg gcatccttgc taaccaacaa tgcaaaatag
    24961 gcaaaaaaat gcccgccccg gcgatcccag agcaggcaga caaaacatac ccagtttcat
    25021 gatatgtttt aaagcgaaat tatttgttga gctggaagag cgacaatccc tgcatatcgg
    25081 taaatgcttt atacgatgcc tgcaatgccg tttgctgcat gatgtaagat gaaatagttg
    25141 cattccagtc cacatcaacc agatcgctca tctgctgcgt ttgccctaaa gcgcgatcgc
    25201 tacctaatga atccagcgac tccagttcgt tcagctgcgt gcctaattcc gcgcgaacag
    25261 tcagcacatt gttcagcgag tttttcagtc cgcggttggt tttatccagt gccgcagcgg
    25321 cggtttcttt atccgcttcg ctatccgcga ccggcgtttt cagggctgcg atggcactat
    25381 ccagcatggc aaaaagattg gtttcagaag cgctaccgcc tggttccgct accgcgttgc
    25441 tggtaatact gtcgaaaatt ttgtcacccg tgtgccctat taccatcgaa cgcgaagcat
    25501 cgacctgttg tttaatactt tctgttccgc cgacataatc accgttagcc tcactaaacg
    25561 gcgcagtctc tgttttataa ccggcaaaaa tgtagcgccc gttaccgtca gtggtgttcg
    25621 ccagattcag caactggtca cgaagcccct gaatatccgt agccagcgag gcccggtcat
    25681 cgtcactcaa agtgccattg ctggcgtaga caattttttc ctgagcattc tggatagctg
    25741 tggtgacctg gctaagtaca ctctcttcca gtgacacttt ttgagtggcg aaagtacgcg
    25801 ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag aactacggct tgtgatgctg
    25861 caatgggatc gtcagaaggg ttaacgactc gcttacctgt cgacatctgc tcgccgtact
    25921 tcatccattc cgcctgagaa ttggtgatgc cacgcatgtt ttgctggtac atcatctggg
    25981 tactgaaacg catcttatct ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt
    26041 tgccgtctgc agaacctgcg cattcgccag gtaatactgc tgaaaacgtt gcagatttcc
    26101 gtactcctca tcgagattga caccggaaat cgactgctgc tgattggaaa gctgcgtcac
    26161 cacattacct tgcgtggtgc tactggtttt caacgtcgcg gttttattac cgatatcact
    26221 cactaacgag gcataagcgt cgttaaagga tttcgcaccg cccaccgttt tactgttgct
    26281 ttgcagatcc agcagggcct gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct
    26341 cgccatcgcg attttcgctt cgtcggtgat taatacatcc atgttgacga tggcgtcact
    26401 tactggtttc agcgtgaagc tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag
    26461 accatcaaat gccactttac cgttggcatc cggcgtcacc gtaaaagtgg tattgctggc
    26521 aaggcgggtg acctgccact gattattatc gaacgagatt ttgtaatctg tcgccagtac
    26581 cgcggaggca tcagttaccg tggcaccgat cgcaacgtta ccgttgtttt tcgtgttttg
    26641 cagaaccgcg ggcttaccga tagcaaagaa atcttcaccc tcatcgccgt tggcatcaaa
    26701 tccggctttg tgttgggtgt tgaaagcctc agcaaatgcc agcgccagtt gtccaagcgt
    26761 attacgcgtc tggtccagat cctgagaacg gaatgtcaga atgccgccca gcgacccggt
    26821 attcagtaat ttctccggga tctcaatatt gcctgccgtc ccatcaacat aagcgacagt
    26881 cgtacgagaa gggtcggcgc tggaaggaac tgccgccaat tgccgcgccg tacttccctg
    26941 aaccagtgag taaccattgg ccatcgtgat gttataagtg ccgccatcct gaacgctgac
    27001 ttctacacca acaatctggt ttaattcgct caccagttga tcgcgttgat ccagcagatt
    27061 gttaggtgac gcccctgccc ccacgcctgt caggcgggag atttgatcgt tcaggctggc
    27121 gatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga tattgacctg
    27181 tttgtcctgg tcgcgaagat actgatcggt ggttttaaac tgattcacca acccttctga
    27241 tttcccaatc agcgcctggc gcgctgccgg gtcttccgcg ttactcacca gcgtttgcag
    27301 gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg agagcatatt
    27361 gtcgattttc gacatctgct catagcgggc agtcaggccg ctactttgcg tctgcgccgc
    27421 acgtaactgg ttggtaataa atgcatcata ctcacgctgc acaccagaaa cgtagacgcc
    27481 attgccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa tagtagtttg
    27541 gcgggtatat ccggcaacgt tatagctgga gatattatta cttgctgtat ttaacgccgc
    27601 ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag ctggacatgg aggttcctta
    27661 taagccttca attacagagt attatcggca gcgccccgcc ggacttgagt tattcagaac
    27721 agattatcaa tgttcatact gtaggttttg ctcaccttgt cgcttatcga tttcatctgc
    27781 tgaatcatgt tggtgagttt acgggcatag tgaggatcgg tggcatagcc cgcgtcctgt
    27841 agggcctgcg ccccctgctc cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta
    27901 cgtgttaaca gcccaacgta atccgacaat gcttccagat acgagctgta gacccgaaac
    27961 ttcgctttta ctttcttcgc ttcgccattt tcatattcag tcgtggtgat ttcagtgact
    28021 ggccctttcc agttgccaga ggctttgaca ccaaacaggt tatagctcgg ctcgccgttt
    28081 tcgcggcgga tttgccgttg cccccagcca gattccagcg ccgcctgagc gaggatcaaa
    28141 tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg
    28201 aatgctttac tgtcacctgg cagcgaatca tcgtagttac gtggcacggc cttttgcacc
    28261 agctgcgaaa gcgcctgatt ttgataacgc accacggttt cgagcgggaa tttcatcggt
    28321 gctgctggcg tggactcctc tggcaatggt tgttctggcg tcatctgctt aaccatcatc
    28381 tctgcaagcc ccagaccttt gcccgccgtc atctgttggg caatctgctg gtcatacata
    28441 ctggtataca ggcgagtgtg ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc
    28501 atgcttttca acatcatctg cacgaacatc ccttccacct gacgggccac cggacggata
    28561 tttgccgccg ggtcttcgcc cgctttcgcc tttagttcgt tgagtgattg cgcatcccag
    28621 gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt tttgcccgca
    28681 gacatcccgc actttgcatt gattgcagta tggacattaa atccattggg gtggctccca
    28741 aggcgttgag cgcgcgcacc acgttattga ggctggcgct ggaacgtacg ctttgcagcg
    28801 aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt aaccacagtc tgtccaccac
    28861 caaacggtgt atctggctgg ctgacattgg cctgacgatt gactgtaact gagagattcc
    28921 cctgcgctac cgcgcagctg tcgagggtca cttcgcgatt catcaccacc gaaccggtgc
    28981 gcgagttaat cactacttta gcgtcctgcg gggtgacatt aacctgcata ttctggatat
    29041 cggcaaggaa acggacctgg gagctgttgc cacttggtac gcgcacctga atagtccgcg
    29101 catccaacgc ggtggcgctg ccatatccac gcactcggtt gatggtgtca gcgatttgct
    29161 gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg acgccaaact
    29221 ggctgggtaa ttcacgttca ataactgcac cattggtgat ccgtccaccg ttcagttggt
    29281 tcacctgaac actgctaccg ccagcggagg ctccagcgcc gccaaccaga atattgccct
    29341 gtgccagcgc atacacctga ctgtcaacgc ccttaagcgg tgtcatcaac aacgtacctc
    29401 cacgcaagct tttggcattt cccatggaag aaaccaccac atcgatggtt tgcccctgac
    29461 gtccaaacgg aggaagtgac gctgtcacca ttaccgcagc gacgtttttt agctgcatat
    29521 tggtgcccgt cggaacggta attcccagct gtgagagcat gttattaagc gtttgtgtgg
    29581 taaacggcgt ctgggttgtc tggtcaccgg tgccatccag ccccaccacc agaccatagc
    29641 caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata cgctcagcct
    29701 gagccgccgt cgtaaccagt agaagaatta atgcagagag aaatttaatc accacagcct
    29761 cacttacatt ggcgacaggt taaggaagaa acgctgcaac cagcccatat tttgcgcttc
    29821 gttaatgtag ccattgccta cgtattcaat gcgcgcatcc gccacctgag tagacggtac
    29881 ggtattgctg ccgctgatgg tgcgtggatt aaccacgcca gagaagcgaa taaattcggt
    29941 accctgatta atggcaatct gtttttcacc caccacatgc aggttgccgt tgaccagtac
    30001 ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg ctggcattgg ccccgccctt
    30061 tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac caaacagccc
    30121 ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt
    30181 cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc
    30241 aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa tcggctgagc
    30301 agactggaaa atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt
    30361 cgccccctgc accagcggcg tggagggtat ccaggcgcag ccggttagtg aaagcaccaa
    30421 caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca tcttcaaaaa
    30481 tcaataaacc ggtgaacctg ccaccggtta tgccttagag ttgcgtcagt ttttgcagca
    30541 tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc acctgaatca
    30601 tattgaccag ttcttccgcc acattgacgt tagacgtttc aacataccct tgatacagca
    30661 gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg
    30721 tgtagaggtt ttcgccaatg ctctccagcc cggtgtcatt cataaaggtg gtgagattga
    30781 gctgcccaac ctgaaccgga gctgcctggc cttgttgggt tacgctgacc acgccatcac
    30841 gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc acctgaaaac
    30901 caccagccgt caccagctgc ccgttctgat ccacctggaa agagccgtca cgggtatagg
    30961 ctgatgagcc atctggcaac atcacctgga aaaagccctg ccctttaatc gcgacatctt
    31021 tgctgttgtt ggtctgcgac aggttgccct ggctgtgtaa gcgttcagtg gcgaccgggc
    31081 gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa gactgtgccc
    31141 ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga cgcttaaaac
    31201 cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt tgctgggcgt
    31261 caaggcccgt tttggcgatc cataatgaac tgatcataaa atgtcctgta gtggattagt
    31321 gacaaagtgc atatgtcttc ttgccggatg cggcgtaaac gccttatccg gcctacaagt
    31381 gctcgcaaaa tcgataaatt gcgattgccc ccatagaccc gataaacttg cgcatcgggc
    31441 aacttatact tgtcatgtat cctttcaatt aactcatcga cagcagttgg ttggcacggc
    31501 ctgcgttatc atcgacgctg ctgatcacct tcatctgcat ttcaaaacgc cgcgcgctgg
    31561 caatcatgtc gctcattgcc gcaacggtat tgacattact gccttccaga acacccgaca
    31621 tcacacgcaa ggttggatct gcctgcagta caagcccacg cgtggcctgg gtttctgcgc
    31681 ttaaacgaaa aatgccgtcg tcaccgcgct gcacttcgct gcccgtggct ttcaccagtt
    31741 taagacgccc tactggcgca accgtatttg ccggatcgcc cggattcagc gccgagattg
    31801 tgccatcggc agcaatagtg atttccgccc cttccggcac agcaattggc cccgcctcgc
    31861 ctatcaccgg atgcccctga attgtcagtt gcccggtggg atcaacctga atgctgccat
    31921 tacgcgtata cccttcgctg ccgtcagcgg tctgcacggc cagccagcca tcctgctgca
    31981 atgcaacgtc cagcgggcgc gaggtgtaat ccatcttgcc gggcgtcatg tctgcacccg
    32041 gtgttgacgc cgttaccagc gtgcgcgtgg gcagtgaaag cccttccacc ggcactgcgc
    32101 gcaaggcgtt taactgcgcg cgaaaaccgg gcgtagaggc attggccaga ttactggcgg
    32161 ttaccgcctg ttgattcagc gtctggctgg ctgctcccat cgcggtataa attgcgtgat
    32221 ccattgagct atcccgtcag cgattagcgt aagttaacca gcgtgttgag gatctggtcc
    32281 tgggttttga tggtctgggc gttagactga tagttacgct gggcaacgat catattgacc
    32341 agttctttac tgagatcgac gttggacgct tccagcgcac cgttggtcag ggtgccaaag
    32401 tttcccgtcc cggctgtccc caacagcgcc acgccagaag attgcgtcgc agaccagacg
    32461 ttgtcgcctt cggatgccag accttcgttg ttggcaaagt tcgccagtac aatctgcccc
    32521 agcagttggg tttgttcatt ggaatagttg ccgacaaccg taccgtcatc attgatttga
    32581 taactcacca gatcgcccgg tttgtagcca ttctgggtgg ttgccacaat gttgttagcg
    32641 ccggtatttt gctgcatgga gttgaggaag ctcaggctaa acgtggcggg ttctgcaccg
    32701 ttaattgcgc cggttgcgat attattcgcc atcgcaccat ccactaatgt gccattagca
    32761 ttaaatttca gtgttgtcgc tgtcttcgca atgctgtttg gatcactgct atcctgggtg
    32821 tagacctgcc agttattatc cccggtcttc acaaagtaca cgctcatgtc atgagcatta
    32881 ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc attgctggca
    32941 tcaaatgcgt taacagaggg aagcggatcg ctggaattca ggttgatctg catcgacgcc
    33001 gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatattggt cggattcgcc
    33061 ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg taaaccttgc
    33121 atattcacca ggttacgatt ttcatccagc ttaaattgtc cgttacggct gtagaacacc
    33181 gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac gtccagacct
    33241 cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc aacttttacc
    33301 cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc tgatttaaag
    33361 ccgtatgtgg cggagttggc gatattgttg ccaataacat cgaggttggt ggcggcagcg
    33421 tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt gaaggcttaa
    33481 attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc cagcgtatta
    33541 ccgctgttgc cgcggatcac accctgcacc agagcaaact gcagcggctg ggcaaccagt
    33601 tgtgtaccac cgttactggc gctaatcgct acattgtaag aaccgttcgg cgcagtgctg
    33661 ccatcagtca acgtaccgtc ccaggtgaaa ctgtgaactc cggcggtcag ttcaccaata
    33721 tcaatggtgc gcacaaccgc gccattttta tcggtgatgg tggccgtaac tttgtctgcc
    33781 gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagccccttc ttcactgccg
    33841 gttcccgcaa gaacagtggt gccggggatc atcacgccgt gaccgatcag gttactggcc
    33901 tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagcgtggt attgagtttt
    33961 tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt gttttccatt
    34021 ggattggtcg ggtcctggtt tttcagctgc gccaccaaca aagtcagaaa actgctttgt
    34081 aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac gccggtattt
    34141 gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga ccgagcgtaa
    34201 gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacattggcc tgatagctgc
    34261 gtgacgccga catggtgtta accatctctc cgacaacatc aacgttcggc atttttacgt
    34321 agccctttgc atccgccagc ggattacccg gttcataaac cagtttgtcc ggggcctgac
    34381 tttctataac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca gcgttaacct
    34441 gaaataccac ctgttttgcc cgatatggct gtccatcggg accggtcacg ctatcagcat
    34501 tcgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac gccgacccgg
    34561 cgatatcgaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa acgttcatca
    34621 tgcctttgat ttgcccgctc aacgcactaa ggctcatctg gtattgcagg ctgttatcgg
    34681 caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa ggctggtccg
    34741 gaatacggta ttgcagttct gcggtaggag tcgtcagcgc ctgcgccgga atgtgttgcg
    34801 ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc atgacttttt
    34861 taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg gcattggcga
    34921 tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct tgttgaaaac
    34981 gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa ttctgccatc
    35041 agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg gtcgctattt
    35101 atgccgttga tggtcattgc ggacaggtac aattcacgtt gtagaaatgg ctgggggcga
    35161 aaatgctggc aataaaacgt agcgtggcga tcatcgcgat actgttcagt ccgttaagtg
    35221 cggcgagcaa tctcacatcg caattgcaaa ccttttttag ctcccagctc gcagggataa
    35281 gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgccg tgcgagcagc
    35341 cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct
    35401 gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat tatgtggttg
    35461 ccgcgatgcc cattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctaaaacgcg
    35521 gacggctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt gttgatgccg
    35581 ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat
    35641 ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg tttagcgcca
    35701 acgctgaagg tcaggcgctg aacaatgcat ccgtcgcaca gaatgcgcgg gtgcgcatgg
    35761 tatctggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt ataaacctgt
    35821 aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc
    35881 tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagccc gtaagcaccg
    35941 ttcaaccgcg cgaaaccact gacgcgccgg taacgaacac ccgggcggca aaaacaacag
    36001 catccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg caacccggca
    36061 gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac
    36121 taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag caagacttgc
    36181 agagtaatta atcgtatgac gcgtcttgca gagatcctcg accagatgtc cgctgtgctt
    36241 aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg gcagatcaac
    36301 ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcaac gttggattac
    36361 ctcgaacagt tacgcaggaa agaacccaat acagcaaata gcgttgatat tagtcaacgc
    36421 tggcaggaaa ttgctgtgaa aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg
    36481 ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag
    36541 gaaccgacgc tatatggggc gaacggtcag acctcaacaa cccatcgcgg cagtaaaaag
    36601 atttcgatct gaagattatc tccggcctgc actgcaggcc ggaatgcatt gttacaccgt
    36661 ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga agtacgcggc
    36721 aatccccgcc agcacgaccg ccattaaacg cagcaagcgc cagggcatgg tacccaatga
    36781 ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca gtaccgccac
    36841 caccagacgc aacagaaacg ccatccagcc aggttgcggg gtaaagattt tctgcttacg
    36901 caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag aaagtgacag
    36961 cccggcatgt ttcaacggac cgataaacgc caggttcatc aattgcgtta aaatcagcgt
    37021 aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc caggagccaa
    37081 cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg cccgctgggt
    37141 catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg tcaacggacc
    37201 tgaaagaatg cccaacgcaa ccgcactcgg cagcgccaac aggaaacaaa gacgcagccc
    37261 ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt tcgacagcga
    37321 cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gacggaaact ccattaagcg
    37381 gtcggcgtaa tacatccacg acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt
    37441 gatgattaag gagatctggc tcacagagac gccaaggatt gccggtccca tctgtttcac
    37501 cacacgcatt gctccggcat cgtggaagtt aatgcgtggc aaaaccagca tgccgatctt
    37561 cttcaggtgc ggtagctgat acaccagctg caggacgccg ccgaccgtaa cagcccacgc
    37621 cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac caatcatgct
    37681 gatgttaagc agtgttggag caaacgccgg aatcgagaag cggttccacg tattcagaat
    37741 cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa tctttagtag
    37801 ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat
    37861 cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcga gtgtcagcag
    37921 gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac ttttatattc
    37981 cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa
    38041 gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc caaagattct
    38101 ggcgacaatt gcgtcacgtg cgaagccaag cacacgcgaa aacatggtca tcgagctgac
    38161 ggcggccagc gattttaata aattcatcgg tgttctaatc cagacccacg ggcaaaacgc
    38221 ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg attatggtga attgctaccg
    38281 ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca acgatacgtt
    38341 gcgccagcac ggcctgttcg ccggcggttt gcggaactgt ctgattttgc acacattcaa
    38401 tgaagtgccg cgcacagccg acaaatccac gttgctcaag cgtgctctgc caaccaggaa
    38461 tcggtttatt cactacaccc tgcccacgct cctcacgcca ttcgcgcatt tccgtaatgt
    38521 cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg gcacggcgat
    38581 gcatacaggt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca aacagcattt
    38641 cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg cccgacaacc
    38701 acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa agatcgtgtg
    38761 gcccggcgct attgctacga tgtttatcca ttcttagcga ggctgcggtg gcgagttgcg
    38821 tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt
    38881 ttttccgcgc cgccagttcc accagccgtt cagcatcgcg cagattttct gccagcggtt
    38941 tatcgacaca gacatgtacc cccgcattga gtaacgtact gaccacgtca aagtgactgg
    39001 cggtgctgga atgtacaaaa accgcatcgc aactggcggc aaggctggat aacgaatcgg
    39061 cataaggaat gcgccagctt tcacaaattg gcagggcttt cgcgcgcgta ggcgaccagg
    39121 ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct ttttgcgcaa
    39181 tgccgcctaa tcccactacg ccgatacgta attttttcac tttaatctcc caggtgggcc
    39241 agcaacgaat caagacgctg tttcagttct gccacttcga tttccagggc ttcgacgcgg
    39301 gcctgtaaat caccgtcaac cgcgttcgac atatccgtta ccgccggctg atcctctacc
    39361 tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc
    39421 accacaaaag gaccatcttc gcgatttgcc agttgttcca gcgtcgactc cacttccgcc
    39481 atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca
    39541 ccacgcaata acaacgtggt gattaacgcc acttctgcgg cgctcagctt cagatcgcca
    39601 aattctgaat tacaaaaacg ttgttcatat ttggtgactc gattaccaaa gccactcacc
    39661 gtgcgcagat aatggcgttt taccagatta tccagttgct cctgcacctc ggattcactc
    39721 agattcatga ccggttcacg gttcgttttc tgattacagg ccgttactac accattgact
    39781 gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcagcc aatcacgcgg
    39841 gcttccagag cagtaagttg atatttcatg agattctcct tagcggccgg gcgtccagtc
    39901 tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca acagatagtc
    39961 tttcgcatag ccttcttttt caaaacccag tcgtgccagt aaatcaccgc tgcgtttatt
    40021 gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca tataacgaat
    40081 ggctgcggtc agagcttcaa acatgagtcc tttgccctgc catttttgcc caatcgaata
    40141 accgagatag caggcatgaa aagagccgcg aacaacattg gaaaaattgg caacgccaat
    40201 aatctctttt tcgtccgggt cgaataaacc aaagtagaaa gctgaacctt gtttatgaaa
    40261 ttcgttaatc atccccagcc tggcctgcca gcctgatgga tagcagtggc tttcgtcgcg
    40321 cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg ccagacgcca
    40381 ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc gcacttttgg
    40441 cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt ctttaatacg
    40501 ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtcccaaa tgccattatc
    40561 tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga taaaaaaata
    40621 ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag tgctcatctt
    40681 ttatttcccg ggaggggaaa tgtcccgcgt gtcgcaggcg aggagcctgg gtaaatattt
    40741 cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc
    40801 tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg ctctcggtct
    40861 acgccaattt attcagcaag gtctgggtat tttcggcggc gcaattgccg accgctttgg
    40921 tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg ccacaatggg
    40981 tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac tcggcggcac
    41041 gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac aacagcgtgg
    41101 tcgttttttc tcgctgttga tgatgcagga cagtgccggt gcggtcattg gcgcattgtt
    41161 ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg cagttctatt
    41221 tgtgctgtgt gcggcgttca atgcgtggtt gttaccagca tggaaactct ccaccgtacg
    41281 cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt
    41341 tctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc tgccaattat
    41401 ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg
    41461 tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt ttcgtctgga
    41521 acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg tgggcatggt
    41581 cagcggcctg caacaacttt ttaccctgat ttgtctgttt tatatcgggt cgatcattgc
    41641 cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc gcggcagcta
    41701 tatggggttt agccgtctgg gtctggcgat tggtggcgct attggttata tcggtggcgg
    41761 ctggctgttt gacctgggca aatcggcgca ccaaccagag cttccgtgga tgatgctggg
    41821 cattattggc atcttcactt tccttgcgct gggctggcag tttagccaga aacgcgccgc
    41881 gcgtcgtttg ctggaacgcg acgcctgatt tgatactccc ccctgactcc tccatactga
    41941 aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg atcactgccc
    42001 ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg aattacatgt
    42061 tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac aggttcccat
    42121 tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gatatcgttc actatgtcga
    42181 taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg aagagtggct
    42241 gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca aatcggcatt
    42301 tgatgagttt tctactcccg ccgcgtgcaa atatttcgtc gacaagaaag aggccagcgc
    42361 gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata tcagcgatga
    42421 tttacgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga
    42481 agatgatatt cagctatttc cactactgcg taatctgacg ctggtagccg gaatcaactg
    42541 gccaagccgc gttgctgatt accgcgataa tatggcgaaa cagacacaaa tcaatttgtt
    42601 atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg ggttgccgcc
    42661 gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg ttgtgtcagg
    42721 atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt gccgctgcat
    42781 tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc atcaccgaac
    42841 aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc ggtttacccg
    42901 gcgtggctga cgcccatatt gttctgacaa acctgaccag ccaaattggt cgcgaagagc
    42961 cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg ttcggtagtc
    43021 agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa gaaaaaggtg
    43081 cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa
    43141 cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac tttaaccagc
    43201 aacctgctta cgtcctgcgc gaagatggca gccagggcga agcaatggcg aaaaaactgg
    43261 cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc atttactgat taatcacgag
    43321 ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt
    43381 ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca tcccaggtat
    43441 taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac atgttaaaaa
    43501 ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc aatctggctc
    43561 cgcccgtgac caccgttgag gctgccgtgg cgtatcgcca acgtattctt gacgccgtac
    43621 ctgccgggca cgatttcacc ccattgatga cctgttattt aacagattcg ctggatccta
    43681 atgagctgga gcgcggattt aacgaaggcg tgttcaccgc tgcaaaactt tatccggcaa
    43741 acgcaaccac taactccagc cacggcgtga cgtcgattga cgccatcatg ccggtacttg
    43801 agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca catgcagata
    43861 tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaaccg ctgcgccagc
    43921 gcctgactgc actgaaagtc gtttttgaac acatcaccac caaagacgca gccgactatg
    43981 tccgtgacgg aaatgaacgg ctggctgcta ccatcactcc gcaacatctg atgtttaacc
    44041 gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtctaccc atcctcaaac
    44101 gcaatattca ccaacaggca ctgcgtgaac tggtcgccag cggttttaat cgagtattcc
    44161 tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcacctgc ggctgcgcgg
    44221 gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa gagatgaatg
    44281 ctctgcagca ctttgaagca ttctgttctg taaatggccc gcagttctat gggttgccgg
    44341 tcaacgacac attcatcgaa ctggtacgtg aagagcatca ggttgctgaa agcatcgcac
    44401 tgacagatga cacgctggtg ccattcctcg ccggggaaac ggtacgctgg tccgttaaac
    44461 aataaaaatc gta
//
LOCUS       DAZKFU020000008        43749 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZKFU020000008 DAZKFU020000000
VERSION     DAZKFU020000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 43749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.008"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..754)
                     /gene="ribB"
                     /locus_tag="ACHOQQ_RS01775"
                     /old_locus_tag="pgaptmp_000355"
     CDS             complement(101..754)
                     /gene="ribB"
                     /locus_tag="ACHOQQ_RS01775"
                     /old_locus_tag="pgaptmp_000355"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGU4468662.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(858..1006)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1128..1427
                     /gene="ubiK"
                     /locus_tag="ACHOQQ_RS01780"
                     /old_locus_tag="pgaptmp_000356"
     CDS             1128..1427
                     /gene="ubiK"
                     /locus_tag="ACHOQQ_RS01780"
                     /old_locus_tag="pgaptmp_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGU4468663.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(1462..1662)
                     /gene="glgS"
                     /locus_tag="ACHOQQ_RS01785"
                     /old_locus_tag="pgaptmp_000357"
     CDS             complement(1462..1662)
                     /gene="glgS"
                     /locus_tag="ACHOQQ_RS01785"
                     /old_locus_tag="pgaptmp_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGU4468664.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            1931..2560
                     /gene="yqiJ"
                     /locus_tag="ACHOQQ_RS01790"
                     /old_locus_tag="pgaptmp_000358"
     CDS             1931..2560
                     /gene="yqiJ"
                     /locus_tag="ACHOQQ_RS01790"
                     /old_locus_tag="pgaptmp_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4468665.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            2587..4248
                     /gene="yqiK"
                     /locus_tag="ACHOQQ_RS01795"
                     /old_locus_tag="pgaptmp_000359"
     CDS             2587..4248
                     /gene="yqiK"
                     /locus_tag="ACHOQQ_RS01795"
                     /old_locus_tag="pgaptmp_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGU4468666.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            4487..4546
                     /locus_tag="ACHOQQ_RS01800"
                     /old_locus_tag="pgaptmp_000360"
     CDS             4487..4546
                     /locus_tag="ACHOQQ_RS01800"
                     /old_locus_tag="pgaptmp_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4468667.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            4863..4922
                     /gene="ibsE"
                     /locus_tag="ACHOQQ_RS01805"
                     /old_locus_tag="pgaptmp_000361"
     CDS             4863..4922
                     /gene="ibsE"
                     /locus_tag="ACHOQQ_RS01805"
                     /old_locus_tag="pgaptmp_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGU4468668.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(5042..6475)
                     /gene="hldE"
                     /locus_tag="ACHOQQ_RS01810"
                     /old_locus_tag="pgaptmp_000362"
     CDS             complement(5042..6475)
                     /gene="hldE"
                     /locus_tag="ACHOQQ_RS01810"
                     /old_locus_tag="pgaptmp_000362"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGU4468669.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQQARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(6523..9363)
                     /gene="glnE"
                     /locus_tag="ACHOQQ_RS01815"
                     /old_locus_tag="pgaptmp_000363"
     CDS             complement(6523..9363)
                     /gene="glnE"
                     /locus_tag="ACHOQQ_RS01815"
                     /old_locus_tag="pgaptmp_000363"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGU4468670.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTVALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSENDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPTHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDVMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(9386..10687)
                     /gene="ygiF"
                     /locus_tag="ACHOQQ_RS01820"
                     /old_locus_tag="pgaptmp_000364"
     CDS             complement(9386..10687)
                     /gene="ygiF"
                     /locus_tag="ACHOQQ_RS01820"
                     /old_locus_tag="pgaptmp_000364"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGU4468671.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAEEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            10929..11549
                     /gene="ygiM"
                     /locus_tag="ACHOQQ_RS01825"
                     /old_locus_tag="pgaptmp_000365"
     CDS             10929..11549
                     /gene="ygiM"
                     /locus_tag="ACHOQQ_RS01825"
                     /old_locus_tag="pgaptmp_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGU4468672.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            11613..12851
                     /gene="cca"
                     /locus_tag="ACHOQQ_RS01830"
                     /old_locus_tag="pgaptmp_000366"
     CDS             11613..12851
                     /gene="cca"
                     /locus_tag="ACHOQQ_RS01830"
                     /old_locus_tag="pgaptmp_000366"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGU4468673.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(13032..13853)
                     /gene="bacA"
                     /locus_tag="ACHOQQ_RS01835"
                     /old_locus_tag="pgaptmp_000367"
     CDS             complement(13032..13853)
                     /gene="bacA"
                     /locus_tag="ACHOQQ_RS01835"
                     /old_locus_tag="pgaptmp_000367"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4468674.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(13944..14312)
                     /gene="folB"
                     /locus_tag="ACHOQQ_RS01840"
                     /old_locus_tag="pgaptmp_000368"
     CDS             complement(13944..14312)
                     /gene="folB"
                     /locus_tag="ACHOQQ_RS01840"
                     /old_locus_tag="pgaptmp_000368"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGU4468675.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            14417..15034
                     /gene="plsY"
                     /locus_tag="ACHOQQ_RS01845"
                     /old_locus_tag="pgaptmp_000369"
     CDS             14417..15034
                     /gene="plsY"
                     /locus_tag="ACHOQQ_RS01845"
                     /old_locus_tag="pgaptmp_000369"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGU4468676.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(15047..15979)
                     /gene="ttdR"
                     /locus_tag="ACHOQQ_RS01850"
                     /old_locus_tag="pgaptmp_000370"
                     /gene_synonym="dan"
     CDS             complement(15047..15979)
                     /gene="ttdR"
                     /locus_tag="ACHOQQ_RS01850"
                     /old_locus_tag="pgaptmp_000370"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGU4468677.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            16186..17097
                     /gene="ttdA"
                     /locus_tag="ACHOQQ_RS01855"
                     /old_locus_tag="pgaptmp_000371"
     CDS             16186..17097
                     /gene="ttdA"
                     /locus_tag="ACHOQQ_RS01855"
                     /old_locus_tag="pgaptmp_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGU4468678.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLSFENLSHTRSAL"
     gene            17094..17699
                     /gene="ttdB"
                     /locus_tag="ACHOQQ_RS01860"
                     /old_locus_tag="pgaptmp_000372"
     CDS             17094..17699
                     /gene="ttdB"
                     /locus_tag="ACHOQQ_RS01860"
                     /old_locus_tag="pgaptmp_000372"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGU4468679.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            17748..19211
                     /gene="ttdT"
                     /locus_tag="ACHOQQ_RS01865"
                     /old_locus_tag="pgaptmp_000373"
     CDS             17748..19211
                     /gene="ttdT"
                     /locus_tag="ACHOQQ_RS01865"
                     /old_locus_tag="pgaptmp_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGU4468680.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLMITGLLWMPVVLL"
     gene            complement(19254..20267)
                     /gene="tsaD"
                     /locus_tag="ACHOQQ_RS01870"
                     /old_locus_tag="pgaptmp_000374"
     CDS             complement(19254..20267)
                     /gene="tsaD"
                     /locus_tag="ACHOQQ_RS01870"
                     /old_locus_tag="pgaptmp_000374"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGU4468681.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            20505..20720
                     /gene="rpsU"
                     /locus_tag="ACHOQQ_RS01875"
                     /old_locus_tag="pgaptmp_000375"
     CDS             20505..20720
                     /gene="rpsU"
                     /locus_tag="ACHOQQ_RS01875"
                     /old_locus_tag="pgaptmp_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGU4468682.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            20831..22576
                     /gene="dnaG"
                     /locus_tag="ACHOQQ_RS01880"
                     /old_locus_tag="pgaptmp_000376"
     CDS             20831..22576
                     /gene="dnaG"
                     /locus_tag="ACHOQQ_RS01880"
                     /old_locus_tag="pgaptmp_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU4468683.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            22771..24612
                     /gene="rpoD"
                     /locus_tag="ACHOQQ_RS01885"
                     /old_locus_tag="pgaptmp_000377"
     CDS             22771..24612
                     /gene="rpoD"
                     /locus_tag="ACHOQQ_RS01885"
                     /old_locus_tag="pgaptmp_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGU4468684.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(24691..25197)
                     /gene="mug"
                     /locus_tag="ACHOQQ_RS01890"
                     /old_locus_tag="pgaptmp_000378"
     CDS             complement(24691..25197)
                     /gene="mug"
                     /locus_tag="ACHOQQ_RS01890"
                     /old_locus_tag="pgaptmp_000378"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGU4468685.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            25322..25397
                     /locus_tag="ACHOQQ_RS01895"
                     /old_locus_tag="pgaptmp_000379"
     tRNA            25322..25397
                     /locus_tag="ACHOQQ_RS01895"
                     /old_locus_tag="pgaptmp_000379"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25355..25357,aa:Ile,seq:cat)
     gene            complement(25451..26215)
                     /gene="nfeF"
                     /locus_tag="ACHOQQ_RS01900"
                     /old_locus_tag="pgaptmp_000380"
                     /gene_synonym="yqjH"
     CDS             complement(25451..26215)
                     /gene="nfeF"
                     /locus_tag="ACHOQQ_RS01900"
                     /old_locus_tag="pgaptmp_000380"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGU4468686.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEDGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            26503..27126
                     /gene="nfeR"
                     /locus_tag="ACHOQQ_RS01905"
                     /old_locus_tag="pgaptmp_000381"
                     /gene_synonym="yqjI"
     CDS             26503..27126
                     /gene="nfeR"
                     /locus_tag="ACHOQQ_RS01905"
                     /old_locus_tag="pgaptmp_000381"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGU4468687.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(27233..28753)
                     /gene="aer"
                     /locus_tag="ACHOQQ_RS01910"
                     /old_locus_tag="pgaptmp_000382"
     CDS             complement(27233..28753)
                     /gene="aer"
                     /locus_tag="ACHOQQ_RS01910"
                     /old_locus_tag="pgaptmp_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGU4468688.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSEMVKHRASRLEDAVTVLH"
     gene            29171..30550
                     /gene="ygjG"
                     /locus_tag="ACHOQQ_RS01915"
                     /old_locus_tag="pgaptmp_000383"
     CDS             29171..30550
                     /gene="ygjG"
                     /locus_tag="ACHOQQ_RS01915"
                     /old_locus_tag="pgaptmp_000383"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGU4468689.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(30592..30924)
                     /gene="ygjH"
                     /locus_tag="ACHOQQ_RS01920"
                     /old_locus_tag="pgaptmp_000384"
     CDS             complement(30592..30924)
                     /gene="ygjH"
                     /locus_tag="ACHOQQ_RS01920"
                     /old_locus_tag="pgaptmp_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGU4468690.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            31143..32126
                     /gene="ebgR"
                     /locus_tag="ACHOQQ_RS01925"
                     /old_locus_tag="pgaptmp_000385"
     CDS             31143..32126
                     /gene="ebgR"
                     /locus_tag="ACHOQQ_RS01925"
                     /old_locus_tag="pgaptmp_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGU4468691.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            32310..35402
                     /gene="ebgA"
                     /locus_tag="ACHOQQ_RS01930"
                     /old_locus_tag="pgaptmp_000386"
     CDS             32310..35402
                     /gene="ebgA"
                     /locus_tag="ACHOQQ_RS01930"
                     /old_locus_tag="pgaptmp_000386"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGU4468692.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHVEVRAEGETLTTRQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGKRYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            35399..35848
                     /gene="ebgC"
                     /locus_tag="ACHOQQ_RS01935"
                     /old_locus_tag="pgaptmp_000387"
     CDS             35399..35848
                     /gene="ebgC"
                     /locus_tag="ACHOQQ_RS01935"
                     /old_locus_tag="pgaptmp_000387"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGU4468693.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            35911..37344
                     /gene="ygjI"
                     /locus_tag="ACHOQQ_RS01940"
                     /old_locus_tag="pgaptmp_000388"
     CDS             35911..37344
                     /gene="ygjI"
                     /locus_tag="ACHOQQ_RS01940"
                     /old_locus_tag="pgaptmp_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4468694.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            37478..38548
                     /gene="ygjJ"
                     /locus_tag="ACHOQQ_RS01945"
                     /old_locus_tag="pgaptmp_000389"
     CDS             37478..38548
                     /gene="ygjJ"
                     /locus_tag="ACHOQQ_RS01945"
                     /old_locus_tag="pgaptmp_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGU4468695.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            38565..40916
                     /gene="ygjK"
                     /locus_tag="ACHOQQ_RS01950"
                     /old_locus_tag="pgaptmp_000390"
     CDS             38565..40916
                     /gene="ygjK"
                     /locus_tag="ACHOQQ_RS01950"
                     /old_locus_tag="pgaptmp_000390"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGU4468696.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKSDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            41736..>43749
                     /gene="fadH"
                     /locus_tag="ACHOQQ_RS01955"
                     /old_locus_tag="pgaptmp_000391"
     CDS             41736..>43749
                     /gene="fadH"
                     /locus_tag="ACHOQQ_RS01955"
                     /old_locus_tag="pgaptmp_000391"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGU4468697.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ANQLQEFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT        10620 a        11840 c        11212 g        10077 t
ORIGIN      
        1 gagaagcgga attaacatct tgcatagcag ggaaaaataa aaaccgaagc ctgctacaac
       61 ttcggtttct tgattaaggc agtaaattaa gcagcggttt tcagctggct ttacgctcat
      121 gtgcctgacg gtatgccacc aggtcttcaa tagtcacaag cgccatattg tgtttattgg
      181 caaactcaat gcactctggt gcacgcgcca tcgtgccatc gtcattagtc agctcacaca
      241 gcacaccagc cggtttgaag cctgccagcg tcattagatc aatagttgct tcagtatgac
      301 cgccacgcgt cagtacacca ccagcctgag cgcggagtgg gaaaacgtgg cccgggcgat
      361 tcagatcaga aggttttgcg ccatcggcaa tcgctgcgcg aacggtcgta atacggtcag
      421 cggcagaaac accggtagtc acaccttcag ccgcttcaat ggtcacggta aaaccggtgc
      481 cataggcgct ggtgttattt tctaccatca ttggcagatc gagttgttta cggcgatctt
      541 cagtaatgca caggcaaaca ataccactac cgtggcgaat ggtcagcgcc atctgctcaa
      601 cagtcatggt ttctgccggg aagatcatat caccttcgtt ttcacggtct tcatcatcaa
      661 gcaccattac accgcgtcct tcacgcagcg cagccagtgc attttcaaca cgttcgaaag
      721 gcgtaccaaa agaggaaagt agcgtctgat tcatggtaaa aaaacctcac taaaattatg
      781 gttaccagaa tcagggcagt cttaggagtg gcggcatata gccaaaataa cgtgagcggg
      841 tccatgcccg acagaatcgt tactctctcc catccggact ctaaccgtcg gccccggaat
      901 tacaccggat ctgctgtcct ttgagttcgc acccaaagcg ctcgcgggct ttcaactgag
      961 ttgatttacc gccggtgggg aatttcgccc cgccctgaga ataagcggat tcactataac
     1021 gctaatgatt agcggcagca acgcatagct tcacataatt ctggtttatg acttaccctt
     1081 atcgcactac aatggcactc aacacctatc agtacaggga aactacaatg attgacccga
     1141 aaaaaattga gcaaatcgct cgccaggttc acgaatcaat gcctaaagga atcagggagt
     1201 tcggggaaga tgtggagaaa aaaatccgcc aaaccctaca agcgcagctg acgcgtctcg
     1261 atctggtaag ccgcgaagaa ttcgacgttc agacgcaagt cctgttgcgg actcgtgaaa
     1321 aactggcgtt actggagcag cgcatcagtg aactggaagc gcgcaataat tcggtagcag
     1381 atcttcaatc tcctccggct atcccgccta ttgataaagc agaataaagc aacacaaaaa
     1441 cgggccagac ggcccgtaat atcagtgttc taattcaagt tctctggttt gcatcatctg
     1501 ttggcaatac caggcataac gtgcgcaaaa ccaggttcta tgttcttcat ccatgttacc
     1561 agtaacggcc agaatatcga ccgggcgacc ttctgcgtgc attctggcaa aactacgcgc
     1621 caggaaatcg aaattattta aagaattaag actatgatcc ataagcacat cctccggtta
     1681 tcgcatacct tttaaggcca tatgcttttt ctgatttcag tattccccaa tcagtgtagt
     1741 gcctttaaac ataacgtgcg taaatatatt gtccccgatc acacttttta gtgaaaaata
     1801 aaaaaccata ttaaaagact ttctcttcca gaacttctct aacatgtgtt attattaaca
     1861 aagacaaatg taagcaagga aactatccca aagtaaatat tgcgtcacta aatggacatt
     1921 ggagtgacat atgattttat tcgccgacta taacacccct tatcttttcg ctatttcctt
     1981 tgtactgttg atcggccttt tagaaatttt tgcgcttatc tgcggtcaca tgctctccgg
     2041 tgctcttgat gcacatcttg accattatga ttccataacc acaggtcata ttagccaggc
     2101 acttcattat ctcaatatcg gaagattgcc agctctcgtc gttctctgct tgctggctgg
     2161 tttttttggt cttattggca tcctgttgca gcatgcctgc atcatggtct ggcaatcacc
     2221 actgtcgaac ctgttcgtgg ttcctgtcag tttactgttt acgattattg cggtgcatta
     2281 taccggtaaa atagttgcgc cctggattcc acgcgaccat agttcagcta ttacagaaga
     2341 agaatatatt ggtagcatgg cattaattac cggtcatcaa gccacttcag gtaatccctg
     2401 cgaaggaaaa ctcaccgatc aatttggcca aatacattat ttactactgg agcctgaaga
     2461 aggaaaaatt ttcactaaag gagacaaagt gttaattatt tgccgacttt ctgcaacgcg
     2521 atatttggcg gaaaacaatc cctggcccca aatattgtaa tttattaatc aaaaggaaat
     2581 ttaataatgg atgatattgt taattctgtg ccctcctgga tgtttaccgc gattattgcc
     2641 gtatgcattc tgtttattat tggaattatt ttcgccaggc tctatcgtcg cgcttcggca
     2701 gagcaagctt ttgttcgtac tggtttaggt gggcaaaaag tggtaatgag cggtggcgca
     2761 atcgtgatgc cgatctttca tgaaataatc cccatcaata tgaatactct gaagctggaa
     2821 gtcagccgct caaccattga tagcctgatt acgaaagatc gtatgcgcgt cgatgtagta
     2881 gtcgctttct ttgtgcgggt aaaaccttca gtagaaggga ttgccaccgc tgcccagacg
     2941 ctggggcaac gcaccctgtc gcctgaagac ttacgtatgt tggttgaaga taaatttgtc
     3001 gatgccctcc gtgcaacagc tgcgcaaatg accatgcatg agttacagga tacccgcgag
     3061 aacttcgtgc agggagtaca aaataccgta gctgaagatc tgtcgaaaaa cggcctggaa
     3121 ctggaaagcg tttcacttac caactttaac cagacctcga aagaacattt caatccgaac
     3181 aatgcctttg acgccgaagg tttaaccaaa ctgactcagg agacggagcg ccgtcgccgc
     3241 gagcgtaacg aagttgaaca ggatgtagaa gttgcggtgc gtgagaaaaa ccgtgatgca
     3301 ctttcgcgca agttggagat tgaacaacaa gaagcgttta tgacgcttga gcaggagcag
     3361 caggttaaaa cccgtaccgc tgagcagaat gcgaaaattg cggcttttga agctgaacgt
     3421 cgtcgtgaag cagagcagac gcgaattctg gctgaacgac agattcagga aacagaaatc
     3481 gaccgcgaac aggccgtccg ctcaagaaaa gttgaagctg aacgtgaagt tcgcattaaa
     3541 gagatcgaac agcagcaggt caccgaaatc gctaaccaga cgaaatcgat cgctattgcc
     3601 gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta accttgcact cgcagaagca
     3661 gtaagcgccc aacaaaacgt tgaaaccact cgccagaccg cagaagccga tcgtgctaaa
     3721 caagttgccc taatcgctgc cgcgcaggat gcagaaacca aagcggttga actgaccgtg
     3781 cgggcaaaag cagaaaaaga agccgcagag atgcaggcgg cggctatcgt tgagttagcc
     3841 gaagctacac gtaaaaaggg tctggcggaa gcagaagcac aacgtgcgct gaacgatgct
     3901 atcaacgtac tttctgatga acaaaccagc cttaaattca aactggcctt gttgcaggcg
     3961 ctgcctgcgg taatagaaaa atccgttgag ccgatgaaat ccattgacgg tatcaagatt
     4021 attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg atgcaaacac gggtaatgtg
     4081 gggggcggaa acctggcgga acaagcgtta tcagccgcac tctcttaccg cacacaggca
     4141 ccgctgattg actccttgct caatgaaatt ggcgtttcag gcggctcact ggcggcattg
     4201 acttcaccct taacctcaac aactcccgtc gcagaaaacg tagaataaat tttcaactat
     4261 gaacatccct ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaactgca
     4321 aatgatcagg ttgttgcaac gttttgcgcg accgctcggg gaaggcaaaa ctgccacagg
     4381 gaaagcattg cgcgaggtcc ggcgctcatc aacaatcggg aagtcagcaa ggggctgaaa
     4441 cgggaaagcc cctcccgaag aaggggcctt acagaaggaa agggttatga tgaagctcgt
     4501 catcatactg attgtgttgt tactcgtaag tttcgcagct tattaacagc caatcagagg
     4561 ggggagaaat cctccctcac ccttgttcct ttactttagg ttgaaaaaaa atcagcgtca
     4621 agaaggtttc attaacacat atccctggcc caaaaagccg gggatttttt atatctgcgt
     4681 tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt tgcgtgaccg ctcggggaag
     4741 gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg ctcatcaaca atcggggaga
     4801 cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg ggccttatag aaggaaaggg
     4861 ttatgatgaa gctcgtcatc atactggttg tgttgttact gttaagtttc ccgacttact
     4921 aacaactcat cagagggggg agaaatcctc ccttaccctt gttcctttac tctaggttga
     4981 aaaaacaaca gcgtcaatag gcctgccatg tacgaagcga gatctgtgaa ccgctttccg
     5041 gttagccttt tttatcctgt tggatcttct tgataatgtt agtggtcgag caaccgtctt
     5101 caaagttgag caccaacact tcgccgccgt tggcccagac ttctttactc ccggcgatct
     5161 cttccggttt gtagtcaccg cctttcacca gcaggtctgg caggatcccg gcgatcagac
     5221 gctgcggcgt gtcttcttca aacgacacta cccagtcgac cgcttccagt gcgcccagca
     5281 caatcatacg ctgttcgagt gggtttaccg ggcgggaatc ccctttcagc cgtttggtgg
     5341 aggcatcgct gttgacggca acaatcaagc ggtcacccag cttgcgggca tttgccagat
     5401 aagagacgtg cccggcatgt aggatatcga agacaccgtt ggtcattacc actttttcac
     5461 cacgtttacg cgctgccgct acagccagct tcagttcctc ttcggtcatc acgccaaagc
     5521 cggtatctgc gcgtccacgt acagcgtttt ccagctcgat cggcgaaacc gtggaggttc
     5581 ccagtttgcc gaccaccacg ccagccgccg cattggcaaa gaagcaggct tcttccagcg
     5641 aattacccgc tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc gcaccggtaa
     5701 cgtcatacac ttcctgcgct tgggttggca tatgcagcgg cgctttaccc ggttgcagca
     5761 gcgacatacc ctgttcggaa cgggtcacta acagagccga gagttcgtaa tcggcaatca
     5821 gtttcatgcc gcgctcaaca atctcttctt cggtcttaca tttaccgaca acagcttcaa
     5881 attccgagag attcggcgtt aacagcgtag cgccgcggta gcgctcaaaa tcggtacctt
     5941 ttggatcaat cagcaccgga acacccgctt tacgcgcctg ttggatcatc tgctgtacgc
     6001 ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc gccaatcgaa ctcagcgcct
     6061 gattaatccg ttcgtgcagc ggttgcggat caacaccttc gaaaccttct tcaaaatcca
     6121 ggcggatcag ctgttggttg cgggaaagta cccgtaattt ggtgatggtc ggatgcgtcg
     6181 gtacagaaac gaagtcgcat ttgacgttga cgtcggccag agatttactc agcgcgcgcg
     6241 ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga gaagcgatat
     6301 tcatcgccac gttagccgcg ccgcccggac gttcttcgat ggtattcact ttaaccacgg
     6361 gcaccggcgc ttccggcgag atacgactgg tggggccgta ccagtaacga tccagcatca
     6421 catcaccaac caccatcact cctgcacgtt caaactctgg cagcgttact ttcattcctg
     6481 tctcctgaga gattcaaaat ttgcgcgcga taataccata cttcattctt ccaccagcca
     6541 cttctgccag cttgcccgca ccaaatcacg ctctgcggtg aagcaatcct ccgacacatg
     6601 gcccggcaat tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg tgtaagcacg
     6661 ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt tgcgccagca attccagaat
     6721 gcgcacgttg tctgaccagc gcgttaactt cggtttttca tgagcgtagc gcaacaccag
     6781 atattgagta ataaattcga tatcggtaat tccgccttca tcggctttga tatcaaagcg
     6841 atcgcgatgt ttattgccga gatgagcgcg cattttttcg cgcatttccc gcacttccgt
     6901 ttgcagagtt ttaccttcac gcggcagcgt cataatctcg cggcgcaccg cgtcaaagtg
     6961 cgcggtgagc tgcggatcgc cgtacactac acgcgcacgc accagcgcct gatgttccca
     7021 cgtccaggcc tcgtttttct gataatcagc aaatgcttct gcggatgtca ccagcattcc
     7081 cgccgccccg gacgggcgta gccgtgcatc cacttcatac aaaatgccgg aagaagtacg
     7141 cgtgctgaac agatgcataa tgcgttgcgc cagacgcaga taaaactgcc gcccgtcgat
     7201 ttcccgctcg ccgtccgtca ttacatccat cgggcaatca tggagaaaga tcaggtcaag
     7261 atcagaactg tagcctaact cccagccgcc cagcttgccg tagccgacca ccgcaaagcc
     7321 gcgaccttca cgttcattca ggtgagtcgg ttttccgtaa cgggcaacca tttgaaccca
     7381 cgcttgctgg accaccgcat ctatcattgc ctccgccagc caggttaagt gatcgctcac
     7441 tttcattacc ggcagcgtac cggcgatatc cgctgccgca atgcgtaaca gttgcgcctg
     7501 tttgaactga cgcagcgcct caagctgttg ttcttcgtca tcttccggca cgcgcagcaa
     7561 atactggcgc aactcatcac ggtaagcatc ggtcgccgtc ggctggtaga gagtgtttgg
     7621 atcgagcaat tcatccagca ataatggata acgcgccagc tggctggcaa tcatcggcga
     7681 cgcggcacac agagaaatca aatgtttaag cgccgcgggg aattcactga gcaattctaa
     7741 ataggtggtg cgggtaacaa tccccaccag caaggcggta atgcgcgaca gcgtgacggc
     7801 agcgtcttca cgcgcacaga catcacttag cagatgtggc atcagatggt cgagcacctg
     7861 acgtcctcgc ggcccgatgg tgcgcttatc cagctctttg cggaaatcag caatcagcgt
     7921 tagcacctgt ttgcgatcat tttcgctaag atgcgccagc actggcgtgg tgtcatcttc
     7981 ctgcaacgca tcctgccaca gctcacgcca ctgttccgac agcgactctt cctgagtttc
     8041 actttcatcg tcgccaatca attcattaaa cacccggcgc acattggtca tatgtgcggt
     8101 cagcgccacg gtcagttgcg gccagtcagc aaagtccatc gcccacgcca gccgcgcacg
     8161 gttaagctca tcagaaggaa gcgtctgggt ttgttcgtcg ttaatgcttt gcagcaggtt
     8221 ttccagacgc cgcaggaaca gatacgccac tcgcaattgt tcagcatcgt tttcagaaag
     8281 cagatgcagc gcggcaatga cgctgagcgt tggcagtaaa gagcgcgact gcagcgacgg
     8341 ttcgcgccca ccgcgaatga gctggaacac ctgaacgata aattcaattt cgcgaatgcc
     8401 gcctgcgccg agtttgatat tgtcggtcaa accacgtcga cgcacttcac gggcaatcat
     8461 ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac ggaaaacaaa
     8521 cgggcgcagc atcgcacgca actcgttagc atagacgcct tcgctatcgc ccataatccg
     8581 cgccttgacc atcgcgtaac gctcccagtc gcgcccctgc tcctggtaat aatcttccag
     8641 cgccgcaaag ctcagcacca gcgggccact ttcgccaaac ggacgcagcc gcatatccac
     8701 gcgatagacg aagccatcct gcgttggttg atccagcact ttaatcagcc gctgccccat
     8761 gcgggtaaaa aactgggcgt tatccagttc ccggcgtcca ccctgcgtac aaccatgttc
     8821 cggccaggca aaaatcagat cgatatcaga ggagaaattc agttccccgc cgcccagctt
     8881 acccatgcct aaaatcagca gcggttgcgc ttcgccctgc gcattgcacg gcgttcccca
     8941 ctcgcggcag caggcgtcat atagccagtc acgcgccgca acaatcagcg tctccgccag
     9001 atagctgagc tgctgcaata tgctctcttc agtaaccagt gccagcgttt gcgcccaggc
     9061 gatacgtacc atgatgcgcc gacggaacag ccgcagctcg cgcattaacc cggcttcgtc
     9121 actcacatta ctgagcgcct cctgcaacca tgcaacgtaa tgctgccatt cgtcggcctg
     9181 cggcggttgg ctttccagtt ccgtcagcca ctctggatgc gcaatcacgc tgtcctgcac
     9241 aaaatcactg aaagtaagta ctgacttcgc ctgtgcgcta agtgattcct cggctaaagg
     9301 ctctggcagc cgctcaacaa cggtctgcca gtactgctgt aacggtgaag agagcggctt
     9361 cataagcgat tttatccttg cctgattaac gttttccgct gtgcaaccag aacggttcct
     9421 gattgtttgc ctcattacgg aaatgttcaa tttcgatgcg ttgcccggtc gcaatagcgt
     9481 gatgcagccc ctgccagttc tccagccagg cttgcgcgac gacaggatca tagtaacccg
     9541 ccagcaacag tattgagtca atatcacgcg tcaggcgtgg caactggtca cggtagcgat
     9601 cgcctaacgg ctggcagaaa acgcttttca gttcagcggc atggcgggaa agatggatat
     9661 cggcaaagcg tttgaaggag tcgctgattt tgccctgcgc tttggcatct aaaaatggct
     9721 gccatgcttt gcttaccaac cattcggtca acgccagctt cgccattgcc gtttcggtag
     9781 agtagaccgc cgtcacggca gaaaccgcag aagcaatggt cgcttcgcat tgggtcagca
     9841 aatcacgtaa gtgagtgctc gctttacgcg gcacaatacc accgaacagc atcagcgtat
     9901 gacggaccag gctaatggct gccagcacct gttctttcgc cgcatcgttg ccgcgtaccc
     9961 acagttcttc atgatactgc cattgcgcta acgccagctc gagcgccgct tccagcccct
    10021 gttccacatc ggcttttgcc gcaacatgca aaatggtggt cggtttgatt tcacgcgccg
    10081 gattgccctg cgccagatga tagccacgcg ccgctttgct caggctgccc tggcgtaatc
    10141 cggtttgcga taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg ccgctaagca
    10201 gttccagttc cagctcacag ataggttcag caaattcctc cgctttcacc tccccctgat
    10261 cgagagcgat ttcaattcgg ctgccatcga ccgccaccag ccatttttcg cgataaaaat
    10321 cggtgctgaa cagcggttgt acgcgggaag cgagatcggc aggcaattcg ccgttcggcc
    10381 agacttccga gggaaactgc gccaggtcga gcgtcggttc gctcaacgcc acgttatatt
    10441 ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc atctcatagc
    10501 gaccgttttc gccacgaata cgtaagccca tatcgtgccc acgcagccag ttatccggcg
    10561 tttcgtagta aatattcagc aactgcacgg ggtcatggtg ctcgccgccc agcgtattga
    10621 gatggtcacg caacgcctca acggcactgt gattaacaat aaactttaat tcgatttcct
    10681 gagccatggc ctttacttat cggttatgtc acataagtga cgatgaacgg cgaacttaat
    10741 gcgatctttt ttgtcagtag atagtatttt gcgccaaatt gccatgcaac gggcaatttg
    10801 acgggcgtaa aagtttgaag cagtggcaag acctgacaca gaggattccg attgatgtcg
    10861 aatgctttgc gtagcgacac tgatgccact actatcgttc cactttccat gacaataacg
    10921 acagcctgat gccaaaatta cgcctgatcg gattaacttt actcgcactt agcgcgactg
    10981 ccgtctcaca cgctgaagaa acgcgctatg tttctgacga actgaatacc tgggtccgta
    11041 gcggtccggg agatcattat cgcctcgtgg gcacggttaa cgccggcgag gaagtgacct
    11101 tattacaaac tgacgccaac accaattatg cccaggtgaa agacagctct ggccgtaccg
    11161 cctggatccc gttgaaacaa cttagcactg agccaagcct gcgctcccgt gtgccagatc
    11221 tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa tatcgataac acctggaatc
    11281 agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc aacgggttaa
    11341 aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc gcagaaaaag gtcgatgccg
    11401 ccagcgtaca gctggatgac aaacagcgca ccatcatcat gcaatggttt atgtatggcg
    11461 gtggcgtgct ggggcttggc ttgctgctcg gtctggtact gccgcacctg atcccaagcc
    11521 gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt cgttgcactg tcttattatt
    11581 aggaattgtg tatttaacga agggatgatg gcgtgaagat ttatctggtc ggtggtgctg
    11641 ttcgggatgc attgttaggg ctaccggtca aagacagaga ttgggtggtg gtcggcagta
    11701 cgccacagga gatgctcgac gcgggctacc agcaggtagg ccgcgatttt cctgtgtttc
    11761 tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac cgaacggaaa tccggttccg
    11821 gttacaccgg ttttacctgc tatgccgcac cggatgtcac gctggaagat gatcttaagc
    11881 gtcgcgatct gaccattaat gcgctggccc aggacgataa cggcgagatt atcgacccgt
    11941 acaacggtct gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc gcttttggcg
    12001 aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc gcgttatgcc cacctcggtt
    12061 ttcgtattgc cgatgaaact ctggcgttga tgcgcgagat gacccatgcg ggtgaactgg
    12121 aacacctgac gcctgaacgg gtgtggaaag agacggaaag cgccctcacc acccgcaatc
    12181 cccaggtgtt cttccaggta ctgcgcgatt gcggtgcact tcgcgtttta ttcccggaaa
    12241 ttgacgccct gtttggcgtt ccggcccctg ccaggtggca tccggaaatc gacacgggta
    12301 ttcatacctt aatgacgctc tcaatggcgg cgatgctgag tccgcaggtc gatgtccgtt
    12361 tcgcaacttt atgccacgat ctcggtaaag gactgacgcc gccagaactc tggccgcgtc
    12421 atcatggtca tggcccggcg ggtgttaagt tagtggaaca actatgccag cgtctgcgcg
    12481 tgccaaatga aattcgcgat ttagccagac tggtggctga gtttcacgat ctcatccaca
    12541 ccttcccaat gctgaaccca aaaaccatcg tcaaattatt tgattccatc gatgcctggc
    12601 gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga ggctgacgtg cgcggcagaa
    12661 ccggttttga atcagcggac tacccgcaag gccgttggtt gcgcgaagcc tgggaagtgg
    12721 cgcagtcagt gccgacaaaa gccgtcgttg aagcgggatt taaaggcgtg gagattcgcg
    12781 aggagctgac ccgacggcgg attgcggcgg tagccagctg gaaggaacaa cgttgcccaa
    12841 agcctgaatg agtattgggc attttgccgg atgcggcgta aacgccttat ccggctacat
    12901 gtcaacgaca gttgtaggcc tgataagacg cgcaagcgtc gcatcaggcg ttgattgccg
    12961 gatgcggcgt gaacgcctta ttcggcctac gcaatcgtgg acaacgccag gcatccaaca
    13021 ctattcctca atcaaaagaa cacgacatac accgcagccg ccacaataaa gcgataaatg
    13081 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    13141 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg ggatatcgcc ggttgtcagg
    13201 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg caccgccagc
    13261 aggaacgaaa actcggaagc agcgtaacgg ctcaccccca tcagcatccc acctgaaatg
    13321 gtcgccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    13381 aatgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    13441 cattcggcgg caatcagcaa caaaccgcca acgaccagcg catacatcac atttatcggg
    13501 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    13561 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    13621 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    13681 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcaaacccc
    13741 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    13801 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    13861 cccctaaatt attaaaatgt accgcttgtc cgaactactg cagtatgacc aggttataac
    13921 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    13981 attacgccaa cattcgccgc ccgcgccact gcgcctggct tgctgagttt gatacgcacc
    14041 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttccgccac gcgctccacc
    14101 agcgcaaaac gtgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtagctg
    14161 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccacgc catttcgata
    14221 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    14281 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    14341 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    14401 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    14461 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    14521 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    14581 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    14641 attaggtgtc agccccttct ggctaggctt aattgccatc gccgcctgtc ttggacacat
    14701 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    14761 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    14821 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    14881 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    14941 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    15001 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    15061 agccttcatc gggtttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    15121 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    15181 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    15241 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcagcaca atctcgccgc
    15301 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    15361 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    15421 cctgtaggga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    15481 ttttattttt tgtcaaaaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    15541 tatccagatc aatattatcc tgcactaaat caatttgtcg atcgaacaat tcaaaatgca
    15601 cctgtaactc aggataattg cgcatcagtt cggtaatggc tggcgcaata tggctgcgcc
    15661 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttgcgtga
    15721 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    15781 gaccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccaacg
    15841 tattttcaag gatttggatc cgcttggtga caaatgcggg cgtctgccca agcgtcgccg
    15901 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaaa tctttggcta
    15961 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    16021 aaaacattgc cattccctgc tctggcgcaa tattcatact tattttcgcg ttgattcacg
    16081 aactgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    16141 tgtggcggtg aatttttcca gacaaataca gaaactggag ttgccatgat gagcgaaagt
    16201 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    16261 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    16321 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    16381 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    16441 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    16501 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtgaa cacaggcaaa
    16561 aataccggca gcggcgtacc gtgggtcacc tgggatatcg tccccgacgg tgacgatgcg
    16621 gaaatcgaag tttacatggc aggcggcggc tgcacgctac caggccgctc gaaagtgtta
    16681 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    16741 gcagtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    16801 gccgccgtgc tctcgcgaaa agccattttg cgcccgattg gcagccgcca ccccaatcca
    16861 aaagcggcag agctggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    16921 caagggctga ctggcaacag ttcagtgatg ggcgtgcata tcgaatctgc cgctcgccat
    16981 ccgtcaacca tcggcgttgc tgtttctaca ggctgctggg cgcatcgtcg cggcacactg
    17041 ctggttcatg ccgatctctc ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    17101 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc ggcgatgtga
    17161 tctacctgac cggtacgctg gtgacctgcc gcgacgtttg tcaccgccgc ttgatcgaac
    17221 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    17281 tgcgcaaaaa cggcgacaaa tgggagatgg tctccgtcgg tccgacaacc agtatgcgta
    17341 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gtcggcaaag
    17401 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    17461 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    17521 ggacagaact cgggatgccg gagtcactgt gggtctgccg ggtcaaagag ttcggcccgc
    17581 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    17641 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    17701 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    17761 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    17821 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtaatcg
    17881 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    17941 tcgccatact ctctccctgg ctgctgttca gccccgaaca gctcgctcag ccaggcttta
    18001 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    18061 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctg gggcgacgta
    18121 tcgcgctgat tctggtgaaa aagatggggc atcgcacgct gtttcttggc tatgcggtga
    18181 tgttctccga gctgatcctg gcacctgtca caccgtccaa ctcggcgcgt ggtgcgggga
    18241 ttatctatcc catcatccgt aacctgccgc cgctctatca atcacaacca aacgacagca
    18301 gttcgcgcag tattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    18361 gcgccatttt cctgacggcg atggctccta acttgctgtt aattggactg atgaaaagcg
    18421 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    18481 tactggtcct gctggttccc tggctggctt acgtgctgta cccgccggta ctgaagtctg
    18541 gtgatcaggt accgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    18601 gtgaaaaacg gatgctgggg ctgatggtag gcgcgctggt gctgtggatt ttcggcggtg
    18661 attatatcga tgccgcgatg gtcggttaca gcgtggtggc gctgatgctg cttctgcgca
    18721 ttatcagttg ggacgatatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    18781 cctcgcttat caccctcgcg accggactca acaacaccgg ttttattagc tggtttggca
    18841 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    18901 tggtgtttta tctactgcgc tactttttcg ccagcgccac ggcgtatacc tccgctctcg
    18961 caccgatgat gatcgccgcc gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    19021 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    19081 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    19141 tttttgggct gatattcctc gtgttgctga tgattaccgg cttactgtgg atgcccgtgg
    19201 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    19261 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa caccgagatc cgccgtcgcg
    19321 cctgctttaa accgcaccat tccggcatag gcgatcatcg caccgttatc agtacaaaat
    19381 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttccgc cagtttcgcc
    19441 cgcagcgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    19501 tccagtgctc gcttacactt aatcatcaac gtatcaacca ccgcatcttc aaaggcgcgg
    19561 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    19621 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    19681 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    19741 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    19801 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    19861 gaaaccagca gcgcaacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    19921 agatgccctt ccatatggtg tacagggatc gccggaacgt tccaggcaaa cgccagagaa
    19981 cgccccacgg tcgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    20041 acagcatcaa tgtcttttgc cgttaaacca gactccttta gcgccgcctg gatcaacggt
    20101 acggttttac gcacatgatc gcgggaggcc agctcaggca caacgccccc gtagtcagcg
    20161 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    20221 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    20281 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    20341 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaaggccgcg
    20401 gtgctttaca aagcagcagc aattgcagta aaattccgca ccattttgaa ataagctggc
    20461 gttgatgcca gcggcaaacc gaattaatca aaggtgagag gcacatgccg gtaattaaag
    20521 tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt caagcgttcc tgcgaaaaag
    20581 caggtgttct ggcggaagtt cgtcgtcgtg agttctatga aaaaccgact accgaacgta
    20641 agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa actggctcgc gaaaacgcac
    20701 gccgcactcg tctgtactaa ttccccgaga gcgttgctct ccgatcagac cgagttgtag
    20761 ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt tcgtttatga attgctaaaa
    20821 atcggggcct atggctggac gaatcccacg cgtattcatt aatgatctgc tggcacgcac
    20881 cgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa aagcagggca agaatttcca
    20941 cgcgtgttgt ccattccaca acgagaaaac cccgtcattc accgttaacg gtgagaaaca
    21001 gttttaccac tgctttggat gtggcgcgca cggcaacgcg atcgacttcc tgatgaacta
    21061 cgacaagctc gagttcgtcg aaacggtcga agagctggca gcaatgcaca atcttgaagt
    21121 gccatttgaa gcaggcagcg gccccagcca gatcgagcgc catcagcggc aaacgcttta
    21181 tcagttgatg gacggtctga atacgtttta ccaacaatct ttacagcaac ctgttgccac
    21241 gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac gaggttatcg ctcgctttgc
    21301 gattggtttt gcgccccccg gctgggacaa cgtcctgaag cggtttggcg gcaatccaga
    21361 aaatcgccag tcattgattg atgcgggcat gttggtcact aacgatcagg gacgcagtta
    21421 cgatcgtttc cgcgagcggg tgatgttccc cattcgcgat aaacgcggtc gggtgattgg
    21481 ttttggcggg cgtgtgctgg gcaacgatac ccccaaatac ctgaactcgc cggaaacaga
    21541 cattttccat aaaggccgcc agctttacgg tctttatgaa gcgcagcagg ataacgctga
    21601 acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg gtggcgctgg cgcaatacgg
    21661 cattaattac gccgttgcgt cgttaggtac gtcaaccacc gccgatcaca tacaactgtt
    21721 gttccgcgcg accaacaatg tcatttgctg ttatgacggc gaccgtgcag gccgtgatgc
    21781 cgcatggcga gcgctggaaa cggcgctgcc ttacatgaca gacggccgtc agctacgctt
    21841 tatgtttttg cctgatggcg aagaccctga cacgctggta cgaaaagaag gtaaagaagc
    21901 gtttgaagcg cggatggagc aggcgatgcc actctccgca tttctgttta acagcctgat
    21961 gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt ttgagtacgc tggcactgcc
    22021 attgatatcg caagtgccgg gcgaaacgct gcgaatatat cttcgtcagg aattaggcaa
    22081 caaattaggc atacttgatg acagccagct tgaacgatta atgccaaaag cggcagagag
    22141 cggcgtttct cgccctgttc cgcagctaaa acgcacgacc atgcgtatac ttatagggtt
    22201 gctggtgcaa aatccagaat tagcgacgtt ggtcccgccg cttgagaatc tggatgaaaa
    22261 taagctccct ggacttggct tattcagaga actggtcaac acttgtctct cccagccagg
    22321 tctgaccacc gggcaacttt tagagcacta tcgtggtaca aataatgctg ccacccttga
    22381 aaaactgtcg atgtgggacg atatagcaga taagaatatt gctgagcaaa ccttcaccga
    22441 ctcactcaac catatgtttg attcgctgct tgaactgcgc caggaagagt taatcgctcg
    22501 tgagcgcacg catggtttaa gcaacgaaga acgcctggag ctctggacat taaaccagga
    22561 gctggcgaaa aagtgattta acggcttaag tgccgaagag cgatcgggaa gcccccgaca
    22621 gccgcactga gaggcagcgg caaatatata agtacgccct cgtaattatc gttggcggta
    22681 aataaccgtt ggatttcagc gttaacggct gaaggacatc gggtcaatcg cccaacacca
    22741 acctcatgaa ataagtgtgg ataccgtctt atggagcaaa acccgcagtc acagctgaaa
    22801 cttcttgtca cccgtggtaa ggagcaaggc tatctgacct atgccgaggt caatgaccat
    22861 ctgccggaag atatcgtcga ttcagatcag atcgaagaca tcatccaaat gatcaacgac
    22921 atgggcattc aggtgatgga agaagcaccg gatgccgatg atctgatgct ggctgaaaac
    22981 accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc tttccagcgt ggaatctgaa
    23041 atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg aaatgggcac cgttgaactg
    23101 ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg aagacgggat caaccaggtt
    23161 caatgctccg ttgctgaata tccggaagcg atcacctatc tgctggaaca gtacgatcgt
    23221 gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg gctttgttga cccgaacgca
    23281 gaagaagatc tggcacctac cgccactcac gtcggttctg agctttccca ggaagatctg
    23341 gacgatgacg aagatgaaga cgaagaagat ggcgatgacg acagcgccga tgatgacaac
    23401 agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac tgcgcgctca gtacgttgta
    23461 acgcgtgaca ccatcaaagc gaaaggtcgc agtcacgctg ctgctcagga agagatcctg
    23521 aaactgtctg aagtattcaa acagttccgc ctggtgccga agcagtttga ctacctggtc
    23581 aacagcatgc gcgtcatgat ggaccgcgtt cgtacgcaag aacgtctgat catgaagctc
    23641 tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta ccctgtttac cggcaacgaa
    23701 accagcgata cctggttcaa cgcggcaatt gcgatgaaca agccgtggtc ggaaaaactg
    23761 cacgatgtct ctgaagaagt gcatcgcgcc ctgcagaaac tgcagcagat tgaagaagaa
    23821 accggcctga ccatcgagca ggttaaagat atcaaccgtc gtatgtccat cggtgaagcg
    23881 aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact tacgtctggt tatttctatc
    23941 gccaagaaat acaccaaccg tggcttgcag ttccttgacc tgattcagga aggcaacatc
    24001 ggtctgatga aagcggttga taaattcgaa taccgccgtg gttacaagtt ctccacctac
    24061 gcaacctggt ggatccgtca ggcgatcacc cgttctatcg cggatcaggc gcgcaccatc
    24121 cgtattccgg tgcatatgat tgagactatc aacaaactca accgtatttc tcgccagatg
    24181 ctgcaagaga tgggccgtga accaacgccg gaagaactgg ctgaacgtat gctgatgccg
    24241 gaagacaaga tccgcaaagt gctgaagatc gccaaagagc caatctccat ggaaacgccg
    24301 atcggtgatg atgaagattc gcatctcggg gatttcattg aggataccac cctcgagctg
    24361 ccgctggatt ctgcgaccac cgaaagcctg cgtgcggcaa cacacgacgt gctggctggc
    24421 ctgaccgcgc gtgaagcgaa agttctgcgt atgcgtttcg gtatcgatat gaacaccgat
    24481 cacacgctgg aagaagtggg taaacagttc gacgttaccc gcgaacgtat ccgtcagatc
    24541 gaagcgaagg cgctgcgcaa actgcgtcac ccgagccgtt ctgaagtgct gcgtagcttc
    24601 ctggacgatt aatcggtagg ccggatcagg cgttacgccg cacccggcac ctgtcccttt
    24661 gcacaaacgc caccttttcg gtggcgtttt ttatcgccca cgcaccacca gcgcctggtc
    24721 cagctcgcga tacgcttcaa ccagtttctc cagtgaaacg cgacttaaac cactgggatt
    24781 tggcagcacc caaatctgcg tcgaaccaat gctgagcgtt tgtttccccc actgtgcacc
    24841 gcgctggctg aatccctgtt catatgcttg tttacccaga atcgccaacg cttgcggctg
    24901 ataatcttca attttttcaa tcagcttacg cccacctgcg tgtagctctt gctttgaaat
    24961 ttcattggct tgcaccgttg gacggtctac cagtttggtg acgccacaac gataatccag
    25021 cagatgctgt gcctcctgcg gcttcaactg acggtcggta aacccggcct gatatatcac
    25081 cttccagaag cgatttgccg gatgagcaaa gggaaaacca gtcccggcgg atgaaagccc
    25141 agggttgata ccgcaaaaca cgacccgtaa ccctggagcc aaaatatcct caaccatatt
    25201 tactcctttc taacatcatc aggaaaagta taacggattg aaaatacatt gtttataaaa
    25261 acagcaggcg cgcggtaatg gctggattgc gacacggagt tactttataa tccgctacca
    25321 tggcccctta gctcagtggt tagagcaggc gactcataat cgcttggtcg ctggttcaag
    25381 tccagcaggg gccaccagat atagcaaagg ctgacgagaa atcgtcagcc tttttctttt
    25441 tatatatcag ttactttgcg tgccagtaag ccgctgcacg tacccgctgt gggtcatact
    25501 gttccgcttc aaagcggcgg cttaaattct taacgacttt accttcgccg gttatccaga
    25561 tgaagtaatc atcggcaggg atttgcatct gcgccagacg cgcatctacc gcctgctcat
    25621 catgtgccag ccattcgata ttaaaaccat caaggtgcgc gagataatcc tgacaggcgt
    25681 tatcccgcac gctaaccagc gcactaactt gcggtttaac ggcaagtttg ctcaacgttt
    25741 ccaggcggcg gcgcaatgcg ggcattccgg attcatcgca gacatacagc tgatacgcgt
    25801 aatcttccgg caccaccagc gaaccgcgcg gacctgccac cgtgagttta tcgcccggtt
    25861 gggcctgcat tgcccagcta ctggcgacgc cgtcttcgtg aataaagaaa tcaatcgcca
    25921 gttcatggcg tagttcgtca tacagtggcg tatagtcacg cgacggtggg cgtggtcctt
    25981 ccggccagac gatgccctct tccgttaccg ttggcggcac aaagtgagcg tcaggttgag
    26041 gaaagaagag tttgctgtga tcgtcaaagc cgcgcgatgt aaaaccgtcc agcgcctcgc
    26101 cgccaaggac aatgcgctga aaaccggcgc tgatacgctc aacgcgtagc acagtcagtt
    26161 cacggaaccg cagatcattg cgaacgcgct gcgggtagcg gggggagtta ttcattgtta
    26221 tcgccttcgt gatggtaatc agatatatct aaataatttt cgcaaatgat aatgattgtt
    26281 aatcatgata aatgcaagcg atttgtagaa ctgatatatc tatagtctga taagacgaac
    26341 cgcctcttct caggcatcat tactcaacgc cggatgcggc gtgaacgcct tatccggcct
    26401 acgtgtgaga tgagtccaca tttaaaaatc atttttattc ttgcaagagt gctcatatca
    26461 gatataaatt agatatatct aattaagcaa aaggaggctg atatgagcca tcatcacgaa
    26521 gggtgttgta aacatgaagg ccagccacgc catgagggct gctgcaaagg tgagaagtca
    26581 gaacacgagc actgcggaca cggtcaccag catgaacacg gtcaatgttg cggtggtcgc
    26641 cacggtcgcg gcggcggtcg tcggcaacgt ttctttggtc acggtgaatt acgtctggtg
    26701 attctggata ttctctcgcg cgatgacagc cacggttacg aattgattaa agcgattgag
    26761 aatctaaccc aggggaatta caccccaagc ccgggcgtca tctacccaac gctggatttt
    26821 ctgcaggagc agtcgctgat taccatccgc gaagaggaag gtggtaagaa gcagattgca
    26881 ctgaccgaac aaggcgcgca gtggctggaa gaaaaccgcg aacaggtgga gatgattgaa
    26941 gaacgcatca aagcgcgttg cgttggtgct gcgctgcgcc agaacccgca aatgaagcgg
    27001 gcgctggata attttaaagc ggtgctggat ttacgcgtca accagagtga tatcactgat
    27061 gcacaaataa aaaagatcat tgcggtgatc gaccgcgccg cttttgatat tacgcaactg
    27121 gattaatcac cgattgccgg atgcggcgta ccctacatac cccgtaggcc tgataagatg
    27181 cgccagcatc gcatcaggca ttgtgctcca accgccgaat cctgcatacc gattaatgca
    27241 gcaccgtcac cgcgtcttcc agtcggctgg cgcggtgttt caccatctcc gacacctgcg
    27301 cactctcttc caccagctcg gcatttttct gggtgatgag gttaagctca tccactgcac
    27361 gggtcaggct ggaaagacca tcggcctgtt ccagcgttga atggctaatc tgggcaatca
    27421 actgggtgac gtttttcacc tgtgccacaa tatcttccat cgtacgtccg gcggcgtgta
    27481 cctgctgcga accggattgc accttatcag cactggcatc aatcagcttg cgaatatcgt
    27541 tggcagcatt ggcgctgcgg ctggctaaat gacgcacttc ccccgccacc actgcaaaac
    27601 ctttgccctg ttcgccggct cgcgccgctt ccaccgccgc attcagggcc agaatattgg
    27661 tctgaaacgc aatatcgtta atcagcgaag taatggtgcc aatgcgctgg gtactgtcgg
    27721 cgatatcgtc catagtcttg atcaccgtgg tcatcgcctc cccgccctgc actgccgcat
    27781 tactggcagt gattgacagt ttatcggcag ccgacgccgt ggcagagttc tgtttcaccg
    27841 acgccgccat ttggttcatg gtcgccaccg tttgctgaac gttatcaact gtctgctggg
    27901 tatgttcgtt cagttcatcg gtgcctttcg ccagcgtctc actgccattt ctgacactgg
    27961 acacctggct tgagacatcg ttaattagcc aacggcacat caggccaagc tgccctaccg
    28021 cgcgtaatgt cagccccagc tcatcgctgc gattcagatg ctcaacacta ttacgctctc
    28081 cggtcgccac cttcagtgcc tgacgggcga cattttctat cgggcggaca atctgccatt
    28141 caaaacaggc gcttgccaac aataccacta acgcacagag gatatacgtc accaccgggg
    28201 cagcaacaaa ccaaagcatg gccgccagca agataaacat cagggtcatc actccacgcg
    28261 cccgccagcg aagcggtaat gaaggcagtt tacccagcca gcctttacgc accaccaggc
    28321 ctttatgaat acgcttaccg gtacgtccgg cgttcagcgc tttgtacagc ggctccaccg
    28381 ccgcgatctc ttcatccgtc gcccgggtac gaatcgacat atagccactg atttttccct
    28441 cgcgcaccat cggtaccgca ttggcccgca cccaataatg gtcaccattt ttgcggcgat
    28501 ttttcacgat gccgctccag ggctcccctt ttttcagggt gaaccacata tctgcaaacg
    28561 ccgcttttgg catatccggg tgacgcacca tgttgtgcgg ctgcccttgt aactcttgca
    28621 aggtatagcc gctcacctgc acaaaagtgt cattagcatg agtgatatag ctttgcagat
    28681 cggtagtgga catcagagtg gtatcgtccg ccagcggggt attttgctgg gtgacatacg
    28741 gatgagaaga catgttcgcg tcctgtgcag gttatatggt tgttaacttc ttgtcagagt
    28801 ttatgtcggc cccgctgcgg ttatctttaa ccgattaatt tgatttagat cgcaatttgc
    28861 gattaaagca caaatctaat tcgttgattt aaaatacttt cactctgtta ctatacgaaa
    28921 acgttaatta tcttgcccaa aaatcaggca attattgccc tgaaaacgtg catttgcgca
    28981 gcaattatca aatccatacc cgacaaaaac cgtgcaaaat aacaacggat gttaacaaat
    29041 agcattaaat attgcgcaaa tgataaccac atttgtgttt atcccgattt tcgcgatcgc
    29101 agccggagtg gcgcaatccc tgcaatactt aaatcggtat catgtgatac gcgagcctcc
    29161 ggagcatatt ttgaacaggt taccttcgag cgcatcggct ttagcgtgca gcgcccacgc
    29221 cctgaatctc attgagaagc gaacgctgga tcatgaggag atgaaagcac ttaaccgaga
    29281 ggtgattgaa tacttcaaag agcatgtcaa tccggggttt ttagagtatc gcaaatctgt
    29341 taccgccggc ggggattacg gagccgtaga gtggcaagcg ggaggtttaa atacgcttgt
    29401 cgacacccag ggacaggagt ttatcgactg cctgggaggt tttggaattt tcaacgtggg
    29461 gcaccgtaat ccagttgtgg tttccgccgt acagaatcaa cttgcgaaac aaccactgca
    29521 cagccaggag ctactcgatc cgttacgggc tatgctggcg aaaacccttg ctgcgctaac
    29581 gcccggtaaa ctgaaataca gcttcttctg taatagcggc accgagtccg ttgaagcggc
    29641 gctgaagctg gcgaaggctt atcagtcgcc gcgcggcaaa tttactttta ttgccaccag
    29701 cggcgcgttc cacggtaaat cacttggcgc gctgtcggcc acggcgaaat cgaccttccg
    29761 caaaccgttt atgccactgc tgccagggtt ccgtcatgtg ccgtttggca atatcgaagc
    29821 catgcgcacg gctcttaatg agtgcaaaaa aaccggtgat gatgtggctg cggtgatcct
    29881 cgaaccgatt cagggcgaag gtggcgtaat tctgccgccg ccgggctatc tcaccgccgt
    29941 gcgtaagctg tgcgatgagt tcggcgcact gatgatcctc gatgaagtac aaacgggcat
    30001 ggggcgcacg ggcaagatgt ttgcctgcga acatgagaac gtccagccag acattctgtg
    30061 tctggcaaaa gccctcggcg gcggcgtgat gccgattggc gcaaccatcg ctactgaaga
    30121 ggtgttctca gtcttgttcg acaacccgtt cctgcatacc accacctttg gcggcaaccc
    30181 gctggcctgt gcggcggcgc tggcgaccat caatgtgttg ctggaacaaa acttaccggc
    30241 tcaggctgaa caaaaaggcg atatgttgct ggacggtttc cgtcaactgg cgcgggaata
    30301 tcccgatctg gtgcaggaag cacgtggtaa agggatgttg atggcgattg agtttgttga
    30361 taacgaaatc ggctataact ttgccagcga gatgttccgc cagcgcgtac tggtggccgg
    30421 aacgctcaat aacgccaaaa cgatccgcat tgaaccgcca ctgacactga ccattgaaca
    30481 gtgtgaactg gtgatcaaag cggcgcgtaa ggcgctggcg gccatgcgag taagtgtcga
    30541 agaagcgtaa tatcaaatcg gatggcgata cgacgtcgcc atccgatctt tttatacgat
    30601 acgtacgccc gcaggcatca tccgctccgg cgttaacagc acgctttcac tgccgtcatc
    30661 ggtttccgca cacaacagca tgcattccga cgtttcaccg cgcattttcg ctttttgcag
    30721 attgcataag accaccaccg tttttcccat caactcttct tcgctgtagt acggcaccag
    30781 gctggtcacg gtttgcagtg ttttttcccc cacatcaacc tgtacgatgt acagcttgtc
    30841 ggcgttttca tggcgtttca cttccacaat ctttccgacg cgcatttcca gacgtgcaaa
    30901 atcagcgtaa gccacggttt ccattgcttc ctcccttcga gtaaaatttt actaaactat
    30961 agcaaaagtt tttctcaatc ctgtaggcta aaaatggaga atgcaggcgt gatcacattc
    31021 gtgagccgct gtgttaccgt tacagcgtca aagaaacgcg ctttatttac tgaaaacagg
    31081 tgacccgata agcacttcct ctacaatggg ggcgcacatc agggaaagta aaaaaggtaa
    31141 acatggcaac actaaaagac atcgcaatcg aagctggcgt atccctggcg acagtatcca
    31201 gggtcttaaa tgacgatccg acattgaatg tgaaagaaga gacgaaacat cgcattctcg
    31261 agatcgccga aaagctggag tacaagacca gtagcgcccg taaactacag acaggcgcag
    31321 tcaaccaaca ccatattctg gctatctaca gctaccagca ggagctggag atcaacgatc
    31381 cttactatct ggcgatccgc cacggcattg aaactcagtg cgaaaagctg gcgatcgaac
    31441 tgaccaactg ttatgaacac agcggattac cagacattaa aaacgtcacc ggtattttaa
    31501 ttgtcggcaa acccacaccc gccctgcgcg ccgccgcctg tgcgttgacc gacaatatct
    31561 gttttatcga ctttcacgaa cccggcagcg gttacgatgc ggtggatatc gatctggcac
    31621 gcatcagtaa agaaatcatc gacttctata tcaaccaggg cgttaatcgc attggtttta
    31681 ttggcggtga agatgagcct ggcaaggcgg atattcgtga ggtcgccttt gcggaatatg
    31741 gccgtctgaa acaagtagta cgcgaagagg atatctggcg cggcggtttt tccagttcgt
    31801 cgggttatga actggcaaaa cagatgctgg cgcgggaaga ctatccgaag gcactgtttg
    31861 ttgcttccga ttccattgct atcggcgtac tgcgggcaat tcatgaacgt ggcctgaaca
    31921 tcccacagga tatttcgctt atcagcgtta acgatatccc caccgcgcga tttacctttc
    31981 cgccgctctc caccgtgcgc atccattccg agatgatggg aagtcagggc gttaacctgg
    32041 tgtatgaaaa agcccgcgat ggtcgcgcgc tgccgctgtt agtcttcgtt cccagcaaat
    32101 taaaactgcg cggcacgacc cgttaaatcc ccttacacac tgtccggcaa tcatttttgc
    32161 cggacagtgc tgccgtttat tttcgtgatc cagttaaagt aaatgcattt acctgctatt
    32221 ttttagtaaa aattttacta aactccccag caattacaca aactaccatc accatgaatg
    32281 gttccgattt ctctctaccg ggaggcccta tgaatcgctg ggaaaacatt cagctcaccc
    32341 acgaaaaccg acttgcgccg cgtgcgtact ttttttcata tgattctgtt gcgcaggcgc
    32401 gtacctttgc ccgcgaaacg agcagcctgt ttctgccctt aagcggtcag tggaatttcc
    32461 acttttttga ccatccgctg caagtgccag aagccttcac ctctgagtta atggctgact
    32521 gggggcatat taccgtcccc gccatgtggc aaatggaagg tcacggcaaa ctgcaatata
    32581 ccgacgaagg ttttccgttc cccatcgatg tgccgtttgt tcccagcgat aacccaaccg
    32641 gtgcctatca acgtattttc accctcagtg acggctggca gggtaaacag acgctgatta
    32701 aatttgacgg cgtcgaaacc tattttgaag tctacgttaa cggtcagtat gtgggtttca
    32761 gcaagggcag tcgcctgacc gcagagtttg acatcagcgc gatggttaaa accggcgaca
    32821 acctgttgtg tgtgcgcgtg atgcagtggg cggactctac ctacgtggaa gaccaggata
    32881 tgtggtggtc ggcggggatc ttccgcgatg tttatctggt cggaaaacaa ctaacacata
    32941 ttaacgattt caccgtgcgt accgactttg acgaagccta ttgcgatgcc acgctttcct
    33001 gcgaagtggt gctagaaaat ctcgccgcct cccctgtcgt cacgacgctg gaatataccc
    33061 tgtttgatgg cgaacgcgtg gtgcacagca gcgccattga tcatttggca attgaaaaac
    33121 tgaccagcgc cagctttgct tttactgtcg aacagccaca gcaatggtca gcagaatccc
    33181 cttatcttta ccatctggtc atgacgctga aagacgccaa cggcaacgtt ctggaagtgg
    33241 taccacaacg cgttggcttc cgtgatatca aagtgcgcga cggtctgttc tggatcaata
    33301 accgttatgt gatgctgcac ggcgtcaacc gtcacgacaa cgatcatcgc aaaggccgcg
    33361 ccgttggaat ggatcgcgtc gagaaagatc tccagttgat gaagcagcac aacatcaact
    33421 ccgtgcgtac cgctcactac ccgaacgatc cgcgttttta cgaactgtgt gatatctacg
    33481 gcttgtttgt gatggcggaa accgacgtcg aatcgcacgg ctttgctaat gtcggcgata
    33541 tcagccgtat taccgacgat ccgcagtggg aaaaagtcta cgtcgagcgc attgttcgcc
    33601 atattcacgc gcagaaaaac catccgtcga tcatcatctg gtcgctgggc aatgaatccg
    33661 gctatggctg taacatccgc gcgatgtacc acgcagcgaa agcgctggat gacacgcgac
    33721 tggtgcatta cgaagaagat cgcgatgctg aagtggtcga tattatttcc accatgtaca
    33781 cccgcgtgcc gctgatgaat gagtttggtg aatacccgca tccgaagccg cgcatcatct
    33841 gtgaatatgc tcatgcgatg gggaacgggc cgggcgggct gacggagtac cagaacgtct
    33901 tctataagca cgattgcatt cagggacatt atgtctggga atggtgcgac cacgggatcc
    33961 aggcgcagga tgacaacggc aatgtctggt ataaattcgg cggcgactac ggcgactatc
    34021 ccaacaacta taacttctgt cttgatggtt tgatctattc cgatcagacg ccgggaccag
    34081 gcctgaaaga gtacaaacag gttattgcgc cggtaaaaat ccacgcgctg gatctgactc
    34141 gcggcgagtt gaaagtcgaa aataaactgt ggtttaccac gcttgatgac tacaccctgc
    34201 acgtagaagt gcgcgccgaa ggtgaaacgc tcacgacgcg gcagattaaa ctgcgcgacg
    34261 ttgcgccgaa cagcgaagcc cccttgcaga tcacgctgcc gcagctggac gcccgcgaag
    34321 cgttcctcaa cattacggtg accaaagatt cccgcacccg ctacagcgaa gccgggcatt
    34381 ctatcgccac ttatcagttc ccgctgaagg aaaacaccgc gcagccagtg cctttcgcac
    34441 caaataatgc gcgtccgctg acgctggaag acgatcgttt gagctgcacc gttcgcggct
    34501 acaacttcgc gatcaccttc tcaaaaatga gtggcaaacc gacatcctgg caggtgaatg
    34561 gcgagtcgct gctgacccgc gagccaaaga tcaacttctt caagccaatg atcgacaacc
    34621 acaagcagga gtacgaaggg ctgtggcaac cgaatcattt gcagatcatg caggaacatc
    34681 tgcgcgactt tgccgtagaa cagagcgatg gtgaagtgtt gatcatcagc cgcacggtta
    34741 tagcaccgcc ggtgtttgac ttcgggatgc gctgcaccta catctggcgc atcgctgccg
    34801 atggccaggt taacgtggcg ctttccggca agcgttacgg cgactatccg cacatcattc
    34861 cgtgcatcgg tttcaccatg gggattaacg gcgaatacga tcaggtggcg tattacggtc
    34921 gtggaccggg cgaaaactac gccgacagcc agcaggctaa catcatcgat atctggcgca
    34981 gcaccgtcga tgccatgttc gagaactatc ccttcccgca gaacaacggt aaccgtcagc
    35041 atgtccgctg gacggcactg actaaccgcc acggcaacgg tctgctggtg gttccgcagc
    35101 gcccaattaa cttcagcgcc tggcactata cccaggaaaa catccacgct gcccagcact
    35161 gtaacgagct gcagcgcagt gatgatatca ccctgaatct cgaccaccag ctgcttggcc
    35221 tcggttccaa ttcctggggc agcgaggtgc tggactcctg gcgcgtctgg ttccgtgact
    35281 tcagctacgg ctttacgttg ctgccggttt ctggcggaga agctaccgcg caaagcctgg
    35341 cgtcgtatga gttcggcgca gggttctttt ccacgaattt gcacagcgag aataagcaat
    35401 gaggatcatc gataacttag aacagttccg ccagatttac gcctctggta agaagtggca
    35461 acgctgcgtt gaagcgattg aaaatatcga caacattcag cctggcgtcg cccactccat
    35521 cggtgactca ttgacttacc gcgtggagac agactccgcg accgatgcgc tatttaccgg
    35581 gcatcgacgc tattttgaag tgcattacta cctgcaaggg cagcaaaaaa ttgaatatgc
    35641 gccgaaagag acattacagg tagtggaata ttatcgtgac gaaactgacc gtgaatattt
    35701 aaaaggctgc ggagaaaccg ttgaggtcca cgaaggacaa atcgttattt gcgatatcca
    35761 tgaagcatat cggtttatct gcaataacgc ggtcaaaaaa gtggttctca aagtcaccat
    35821 cgaagatggt tattttcata acaaataaca actacggcgg caaaaggagt ttgccgccac
    35881 cgctacccta ctcattttcg gagatgtgtt atgtctgata ccaaacgtaa tacaatcggc
    35941 aaattcggct tgctctcgct gacttttgcc gccgtttaca gctttaacaa cgttatcaat
    36001 aataatattg agcttggact ggcctcggca ccgatgtttt tcctcgcgac gattttttat
    36061 tttattccct tctgtctgat catcgcagaa tttgtttcgt taaataaaaa ctcagaagcc
    36121 ggtgtctacg cgtgggtaaa aagttcgctg ggcggacgtt gggcatttat tactgcctat
    36181 acctactggt tcgtaaacct gttctttttc acctcgctgt tgccgcgcgt tattgcttat
    36241 gcttcgtatg ccttcctcgg ctacgaatat attatgacgc cggttgccac caccattatc
    36301 agtatggtgc tgttcgcctt ctctacctgg gtttccacca acggggcgaa aatgctgggg
    36361 ccaattacct ccgtcacttc aacgctgatg ctgctgttaa cgctctccta cattttactg
    36421 gcaggtacgg cgctggttgg cggcgtacag cctgctgacc ccatcaccgt tgacgcgatg
    36481 atcccgaact tcaactgggc gttcctcggc gttaccacct ggatttttat ggccgcaggt
    36541 ggcgcggagt ccgtcgctgt gtacgttaac gacgtcaaag gcggttcgaa atcgttcgtt
    36601 aaagtgatca tcctcgccgg gatttttatc ggcgtactat attccgtctc ctcggtgctg
    36661 attaacgtct tcgtcagcag caaagagttg aaatttactg gcggatcggt gcaggtattc
    36721 cacggcatgg cggcgtattt tggtctgccg gaagcgttga tgaatcgctt tgtcggtctg
    36781 gtgtccttta ccgcaatgtt cggttccctg ctgatgtgga ccgcaacgcc ggtgaaaatt
    36841 ttcttctccg aaatcccgga aggcatcttt ggtaagaaaa ccgtagaact gaacgaaaac
    36901 ggcgttccgg cgcgcgcagc gtggatccag ttcctgatcg tcatcccgct gatgattatc
    36961 ccgatgctcg gttccaatac cgtgcaggat ctgatgaata ccattattaa tatgaccgcc
    37021 gcagcgtcca tgcttccgcc gttattcatc atgctggctt acctgaattt acgcgccaaa
    37081 ttagatcacc tgccacgcga tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt
    37141 tcaatgctga ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc gactggcgcg
    37201 aatattctga ccatcatttt ttataacgtc ggcggtattg ttatcttcct tggctttgcg
    37261 tggtggaaat acagtaaata tataaaggga ttaacggctg aagagcgcca tattgaagcg
    37321 acgccagcca gcaatgttga ttaaacagac taataaaaaa tgagcaggca ttttccctct
    37381 tcctggatga ggagagggaa aatagtttct gcctttatat ttttataatt acaacgataa
    37441 aaggctgtac tttttcttta gctcatggat taacacaatg aaattaatca ctgcaccatg
    37501 cagagcatta cttgctctgc cgttttgcta cgccttttct gcggcaggag aagaagcacg
    37561 tccggcagaa catgacgaca caaaaacacc cgcaattacc tcgacatctt ctccttcatt
    37621 tcgtttttac ggcgaattag gggttggtgg atatatggat ttagagggtg agaataaaca
    37681 taaatacagc gacggcacct atattgaagg tggcctggag atgaagtacg gctcctggtt
    37741 cggcctgatt tacggcgaag gctggaccgt gcaggccgac cacgacggca atgcctgggt
    37801 gcctgaccat agctggggcg gtttcgaagg tgggattaac cgtttctatg gcggttatcg
    37861 aaccaatgat ggcaccgaaa tcatgctcag tctgcgtcag gattcctcgc tggatgacct
    37921 gcaatggtgg ggcgacttca cacccgatct gggctacgtc attcccaata cccgcgacat
    37981 tatgaccgcg ctgaaggtgc agaacttaag cggcaacttt cgttatagcg tcaccgccac
    38041 acctgccgga catcatgacg aaagcaaagc atggctacat tttggcaagt acgatcgcta
    38101 cgacgacaaa tacacctatc cggcaatgat gaacggttac atccagtatg accttgccga
    38161 aggcatcacc tggatgaatg gtctggaaat taccgacggc acaggacaac tctatctcac
    38221 gggcctgctt acccctaact ttgccgctcg cgcctggcac cataccggac gtgccgacgg
    38281 gttggacgta ccgggaagtg aaagcgggat gatggtgagc gccatgtatg aagcgttaaa
    38341 gggcgtttat ctctccaccg cttacaccta cgccaaacat cgccctgacc acgctgacga
    38401 tgaaaccacc tctttcatgc agtttggtat ctggtacgaa tatggcggcg gacgtttcgc
    38461 aacggctttt gatagccgct tctacatgaa aaatgcctct aacgatccca gcgaccaaat
    38521 cttcctgatg caatatttct actggtaata aggactgtaa tttcatgaaa atcaaaacta
    38581 ttttaacgcc agtaacctgc gctctgctga taagtttttc cgcccatgcc gctaacgccg
    38641 acaattataa aaacgtgatt aaccgtactg gtgcgccgca gtacatgaag gattacgatt
    38701 acgacgatca ccagcgtttt aatccgtttt tcgatctcgg agcctggcat ggtcatctgt
    38761 tgccagacgg ccctaacacc atgggcggtt ttccaggcgt tgcgctgctg acggaagagt
    38821 acatcaactt tatggccagc aatttcgacc gcctgaccgt ctggcaggac ggcaagaaag
    38881 tcgacttcac gctggaggca tacagtattc ccggcgcgct ggtgcaaaaa ctgacagcaa
    38941 aagatgtgca ggtcgaaatg actctgcgct tcgccacgcc gcgcacgtca ctactggaaa
    39001 ctaaaatcat cagcaataaa ccgctggatc tggtgtggga tggcgaactg ctggaaaaac
    39061 tggaagcgaa agaagggaaa ccgctttccg ataaaaccat tgctggcgaa taccctgact
    39121 accagcgcaa aatcagcgcc acccgtgatg gcctgaaagt cacctttggc aaagtgcgcg
    39181 ccacttggga tctgctgacc tccggtgaat cagaatatca ggtgcataaa tccctgccgg
    39241 tgcagactga aatcaacggt aatcgcttta ccagtaaggc gcatatcaac gggtcgacga
    39301 cgctctatac cacctactcc cacctgctga ccgctcagga agttagcaaa gagcaaatgc
    39361 agatccgcga tattctggcg cgtccggcgt tttacctcac cgcctcgcag caacgctggg
    39421 aagagtatct gaagaaaggg ttaaccaatc cggatgcgac gccggaacag acgcgcgtcg
    39481 cagtaaaagc aatcgaaacg ctcaacggta actggcgttc gcctggcggg gcggtgaaat
    39541 ataacaccgt cacgccgtcg gtgaccgggc gctggttctc cggcaatcag acctggccgt
    39601 gggatacctg gaagcaggcg tttgcgatgg cgcatttcaa tccggacatc gccaaagaga
    39661 atatccgcgc ggtcttctcc tggcagatcc agcctggcga cagcgtgcgt ccgcaggatg
    39721 tgggctttgt ccccgacctg atagcgtgga accttagccc cgagcgtggt ggcgatggcg
    39781 gcaactggaa cgaacgtaat accaaaccca gccttgccgc ctggtcggtg atggaagtgt
    39841 ataacgtcac ccaggataaa gcctggttgg cagagatgta cccgaaactg gtggcctatc
    39901 atgactggtg gttacgtaac cgcgatcata acggcaacgg cgtgccggaa tatggcgcga
    39961 cccgcgacaa agcccacaac actgagagcg gcgagatgct gtttacggtg aagaaaagcg
    40021 acaaagaaga gacgcagtct gggctgaaca actacgcccg cgtggtggag aaaggccagt
    40081 acgacagtct ggaaattccg gcacaggttg ctgcatcgtg ggaatcgggg cgtgatgatg
    40141 ccgccgtctt tgggtttatc gacaaagaac agctggataa atatgtcgca agcggcggca
    40201 aacgtagcga ctggacggtg aaattcgccg aaaaccgcag tcaggacgga acgttgctgg
    40261 ggtactcgct attgcaggag tcggtggatc aggccagcta tatgtacagc gataaccatt
    40321 atctggcgga gatggcgacg attctcggta aaccggaaga ggctaagcgc tatcgccagt
    40381 tggcacagca gctcgcggac tacatcaaca cctgtatgtt cgacccggct acgcagtact
    40441 tctatgacgt gcgtattgaa gataaaccgc tggcgaacgg ctgcgcgggc aaaccgattg
    40501 ttgagcgcgg taaagggccg gaaggctggt cgccgctgtt taacggtgcg gcaacgcagg
    40561 ccaatgccga cgcggtggtg aaggtgatgc tcgatcctaa agagttcaat acctttgttc
    40621 cgctgggaac ggcggcgtta accaatccgg cttttggcgc tgatatctac tggcgcgggc
    40681 gcgtatgggt ggatcagttc tggtttggtc tgaaagggat ggagcgttac ggttatcgtg
    40741 atgatgccct gaagctggcg gatacgttct tccggcatgc caaaggatta accgccgatg
    40801 gcccaattca ggagaattac aacccgctga ctggcgcgca gcaaggcgca ccaaatttct
    40861 cctggagtgc cgcacatttg tatatgttgt ataacgattt tttccgtaag caataatttg
    40921 caacggctgg cggatgcggc gtgaacgcct tatccgccct acatattcgt tcccgtaggc
    40981 tggataagac gcgacaagcg tcgcatccgg catctgcacc gcgccaccgg cggatgctgc
    41041 gtgaacgcct tatccgccct acatgttcgt tcccgtaggc tggataagac gcgacaagcg
    41101 tcgcatccgg catctgcacc tcgccactgg cggatgcggc gtgaacgcct tatccgccct
    41161 acatgttcgt tcccgtaggc tggataagac ccgacaagcg tcgcatccgg cgtctgcacc
    41221 gcgccactgg cggatgcggc gtgaacgcct tatccgccct acatgttcgt tcccgtaggc
    41281 tggataagac gcgacaagcg tcgcatccgg catctgcacc gcgccggcgg atgcggcgtg
    41341 aacgccttat ccgccctaca tgtgcaatcc cccaggtcgg ataggacccg gcaagcgtcg
    41401 catccggcat ctgcaccgcg ccggcggatg cggcgtgaac gccttatccg ccctacatgt
    41461 tcgttcccgt aggctggata agacgcgaca agcgtcgtat ccggcatctg caccgcgcca
    41521 ctggcggatg cggcgtgaac gccttatccg ccctacatgt gcaatccccc aggtcggata
    41581 ggacccggca agcgtcgcat ccggcgttac caccgggcgt attctttttg aatcccatca
    41641 caaaccccgc actccccttt tcccttttct ccggcgacgg ctaaattaga actcatccga
    41701 ccacataaca attattttac atactggaca attttatgag ctacccgtcg ctgttcgccc
    41761 cgctggattt aggttttacc acgttaaaaa accgcgtgtt gatgggttca atgcacaccg
    41821 ggctggagga atacccggac ggcgctgaac ggctggctgc gttttatgcc gaacgcgccc
    41881 gtcacggcgt ggcgctgatt gttagcggcg gcattgcacc agatttaaca ggcgttggca
    41941 tggaaggcgg cgcaatgctc aatgacgcca gccagatccc acaccatcgc accattaccg
    42001 aagccgtaca tcaggaaggc ggcaaaatag ccctgcaaat tttgcatacc gggcgctaca
    42061 gctaccaacc gcatctggtc gcaccgtccg cattgcaggc ccccatcaac cgtttcgttc
    42121 ctcatgaact cacccatgaa gagatcctgc aactgatcga cgatttcgcc cgctgcgcgc
    42181 aactggcgcg ggaggcagga tacgacggtg tagaggtgat gggttccgaa gggtatttga
    42241 tcaacgaatt tctgacgctg cgcaccaatc agcgtagtga ccagtggggc ggcgattacc
    42301 acaaccggat gcggtttgcc gttgaagtgg tgagtgcggt acgcgaacgc gttggcaacg
    42361 acttcattat tatctaccga ctgtcgatgc tcgacctggt cgaagacggc gggacttttg
    42421 ccgaaacggt agagctggcg caggccattg aagcggcggg cgcaaccatt atcaacaccg
    42481 gcattggctg gcatgaagca cgtattccga ccattgccac gcccgtgccg cgcggcgcat
    42541 ttagctgggt cacgcgcaaa ctgaaaggcc acgtttcgct gccgctggtg accaccaacc
    42601 gaattaacga tccgcaggtt gccgacgaca ttctctcgcg cggcgatgcc gatatggtgt
    42661 cgatggcacg accgtttctt gctgatgcgg agctgctgtc aaaagcgcaa tcagggcgag
    42721 ccgatgagat caacacctgc attggctgca atcaggcctg cctcgatcaa atcttcgttg
    42781 gcaaagtcac ctcgtgcctg gtgaatcctc gtgcctgcca tgaaaccaaa atgccgatcc
    42841 ttcccgccat gcagaaaaaa aatctggcgg tggtcggtgc gggaccagct gggctggcgt
    42901 ttgccattaa cgcggcggcg cgtgggcatc aggtaacatt gtttgatgcc cacagcgaga
    42961 tcggcgggca gtttaatatc gccaaacaga tccccggcaa agaggagttt tacgaaacgc
    43021 tgcgctacta ccggcggatg atcgaagtaa cgggcgtgac gctgaaactc aatcacaccg
    43081 taacagcgaa tcagttacag gaattcgatg aaacgatcct cgccagtggg atcgtgccgc
    43141 gcactccgcc catcgacggg atcgatcatc cgaaggtatt gagttatctc gatgtactgc
    43201 gcgacaaagc gccggtgggc aacaaagttg ccatcatcgg ttgtggcggg attggttttg
    43261 atacggcgat gtatttaagt cagccgggcg aatccaccag ccagaatatc gctgagtttt
    43321 gtaatgagtg ggggatcgac agtagcctac aacaggctgg tggcttaagc ccgcagggaa
    43381 tgcagatccc gcgtagccca cggcagattg tgatgctcca gcgcaaagcc agcaaaccgg
    43441 gacaggggtt aggcaaaacc accggctgga tccatcgcac caccctgctc tcgcggggtg
    43501 tgaaaatgat cccaggcgta agttatcaga agattgacga tgacgggctg catgtggtga
    43561 tcaacggcga aacgcaggta ttagcagtgg acaatgtggt gatctgcgca gggcaagagc
    43621 caaaccgcgc gctggcgcag ccgctgattg atagcgggaa aacggtgcat ttaattggcg
    43681 gctgcgatgt ggctatggag ctggacgcac gacgggcaat tgcccaggga acacggctgg
    43741 cgctggaga
//
LOCUS       DAZKFU020000009        41895 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZKFU020000009 DAZKFU020000000
VERSION     DAZKFU020000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 41895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.009"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            18..1067
                     /locus_tag="ACHOQQ_RS01960"
                     /old_locus_tag="pgaptmp_000392"
     CDS             18..1067
                     /locus_tag="ACHOQQ_RS01960"
                     /old_locus_tag="pgaptmp_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGU4468698.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1141..1998
                     /gene="nfo"
                     /locus_tag="ACHOQQ_RS01965"
                     /old_locus_tag="pgaptmp_000393"
     CDS             1141..1998
                     /gene="nfo"
                     /locus_tag="ACHOQQ_RS01965"
                     /old_locus_tag="pgaptmp_000393"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGU4468699.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2001..3089
                     /locus_tag="ACHOQQ_RS01970"
                     /old_locus_tag="pgaptmp_000394"
     CDS             2001..3089
                     /locus_tag="ACHOQQ_RS01970"
                     /old_locus_tag="pgaptmp_000394"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4468700.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3145..4395)
                     /locus_tag="ACHOQQ_RS01975"
                     /old_locus_tag="pgaptmp_000395"
     CDS             complement(3145..4395)
                     /locus_tag="ACHOQQ_RS01975"
                     /old_locus_tag="pgaptmp_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4468701.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     MLSPATESSQVSFNNLSFTETPPKSIIEAAATGAVTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4495..5436)
                     /gene="rihB"
                     /locus_tag="ACHOQQ_RS01980"
                     /old_locus_tag="pgaptmp_000396"
     CDS             complement(4495..5436)
                     /gene="rihB"
                     /locus_tag="ACHOQQ_RS01980"
                     /old_locus_tag="pgaptmp_000396"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGU4468702.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5566..6264
                     /locus_tag="ACHOQQ_RS01985"
                     /old_locus_tag="pgaptmp_000397"
     CDS             5566..6264
                     /locus_tag="ACHOQQ_RS01985"
                     /old_locus_tag="pgaptmp_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGU4468703.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6332..7582)
                     /locus_tag="ACHOQQ_RS01990"
                     /old_locus_tag="pgaptmp_000398"
     CDS             complement(6332..7582)
                     /locus_tag="ACHOQQ_RS01990"
                     /old_locus_tag="pgaptmp_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4468704.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFTNASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7676..8614)
                     /gene="psuG"
                     /locus_tag="ACHOQQ_RS01995"
                     /old_locus_tag="pgaptmp_000399"
     CDS             complement(7676..8614)
                     /gene="psuG"
                     /locus_tag="ACHOQQ_RS01995"
                     /old_locus_tag="pgaptmp_000399"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGU4468705.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(8602..9543)
                     /locus_tag="ACHOQQ_RS02000"
                     /old_locus_tag="pgaptmp_000400"
     CDS             complement(8602..9543)
                     /locus_tag="ACHOQQ_RS02000"
                     /old_locus_tag="pgaptmp_000400"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGU4468706.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQSAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNLIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(9967..11658)
                     /gene="fruA"
                     /locus_tag="ACHOQQ_RS02005"
                     /old_locus_tag="pgaptmp_000401"
     CDS             complement(9967..11658)
                     /gene="fruA"
                     /locus_tag="ACHOQQ_RS02005"
                     /old_locus_tag="pgaptmp_000401"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGU4468707.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVASLAYAFLKRPEVDAVAKAA"
     gene            complement(11675..12613)
                     /gene="fruK"
                     /locus_tag="ACHOQQ_RS02010"
                     /old_locus_tag="pgaptmp_000402"
     CDS             complement(11675..12613)
                     /gene="fruK"
                     /locus_tag="ACHOQQ_RS02010"
                     /old_locus_tag="pgaptmp_000402"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGU4468708.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12613..13743)
                     /gene="fruB"
                     /locus_tag="ACHOQQ_RS02015"
                     /old_locus_tag="pgaptmp_000403"
     CDS             complement(12613..13743)
                     /gene="fruB"
                     /locus_tag="ACHOQQ_RS02015"
                     /old_locus_tag="pgaptmp_000403"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGU4468709.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14110..15291
                     /gene="setB"
                     /locus_tag="ACHOQQ_RS02020"
                     /old_locus_tag="pgaptmp_000404"
     CDS             14110..15291
                     /gene="setB"
                     /locus_tag="ACHOQQ_RS02020"
                     /old_locus_tag="pgaptmp_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGU4468710.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15288..15542)
                     /locus_tag="ACHOQQ_RS02025"
                     /old_locus_tag="pgaptmp_000405"
     CDS             complement(15288..15542)
                     /locus_tag="ACHOQQ_RS02025"
                     /old_locus_tag="pgaptmp_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4468711.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15697..16269
                     /gene="yeiP"
                     /locus_tag="ACHOQQ_RS02030"
                     /old_locus_tag="pgaptmp_000406"
     CDS             15697..16269
                     /gene="yeiP"
                     /locus_tag="ACHOQQ_RS02030"
                     /old_locus_tag="pgaptmp_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGU4468712.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16492..17958
                     /locus_tag="ACHOQQ_RS02035"
                     /old_locus_tag="pgaptmp_000407"
     CDS             16492..17958
                     /locus_tag="ACHOQQ_RS02035"
                     /old_locus_tag="pgaptmp_000407"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU4468713.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQSGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            18076..19062
                     /gene="yeiR"
                     /locus_tag="ACHOQQ_RS02040"
                     /old_locus_tag="pgaptmp_000408"
     CDS             18076..19062
                     /gene="yeiR"
                     /locus_tag="ACHOQQ_RS02040"
                     /old_locus_tag="pgaptmp_000408"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGU4468714.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNGLQSALLKLRLATTA"
     gene            19101..19814
                     /gene="lpxT"
                     /locus_tag="ACHOQQ_RS02045"
                     /old_locus_tag="pgaptmp_000409"
     CDS             19101..19814
                     /gene="lpxT"
                     /locus_tag="ACHOQQ_RS02045"
                     /old_locus_tag="pgaptmp_000409"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGU4468715.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFSDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20226..20792
                     /gene="mepS"
                     /locus_tag="ACHOQQ_RS02050"
                     /old_locus_tag="pgaptmp_000410"
     CDS             20226..20792
                     /gene="mepS"
                     /locus_tag="ACHOQQ_RS02050"
                     /old_locus_tag="pgaptmp_000410"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGU4468716.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            20973..22529
                     /locus_tag="ACHOQQ_RS02055"
                     /old_locus_tag="pgaptmp_000411"
     CDS             20973..22529
                     /locus_tag="ACHOQQ_RS02055"
                     /old_locus_tag="pgaptmp_000411"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4468717.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGIEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22611..24425
                     /locus_tag="ACHOQQ_RS02060"
                     /old_locus_tag="pgaptmp_000412"
     CDS             22611..24425
                     /locus_tag="ACHOQQ_RS02060"
                     /old_locus_tag="pgaptmp_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGU4468718.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSAKQQGE"
     gene            24426..25520
                     /locus_tag="ACHOQQ_RS02065"
                     /old_locus_tag="pgaptmp_000413"
     CDS             24426..25520
                     /locus_tag="ACHOQQ_RS02065"
                     /old_locus_tag="pgaptmp_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGU4468719.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPLHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25520..26545
                     /locus_tag="ACHOQQ_RS02070"
                     /old_locus_tag="pgaptmp_000414"
     CDS             25520..26545
                     /locus_tag="ACHOQQ_RS02070"
                     /old_locus_tag="pgaptmp_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4468720.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26547..28136
                     /gene="yejF"
                     /locus_tag="ACHOQQ_RS02075"
                     /old_locus_tag="pgaptmp_000415"
     CDS             26547..28136
                     /gene="yejF"
                     /locus_tag="ACHOQQ_RS02075"
                     /old_locus_tag="pgaptmp_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGU4468721.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(28140..28484)
                     /locus_tag="ACHOQQ_RS02080"
                     /old_locus_tag="pgaptmp_000416"
     CDS             complement(28140..28484)
                     /locus_tag="ACHOQQ_RS02080"
                     /old_locus_tag="pgaptmp_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HGU4468722.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28817..30007)
                     /gene="bcr"
                     /locus_tag="ACHOQQ_RS02085"
                     /old_locus_tag="pgaptmp_000417"
     CDS             complement(28817..30007)
                     /gene="bcr"
                     /locus_tag="ACHOQQ_RS02085"
                     /old_locus_tag="pgaptmp_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGU4468723.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(30035..30730)
                     /gene="rsuA"
                     /locus_tag="ACHOQQ_RS02090"
                     /old_locus_tag="pgaptmp_000418"
     CDS             complement(30035..30730)
                     /gene="rsuA"
                     /locus_tag="ACHOQQ_RS02090"
                     /old_locus_tag="pgaptmp_000418"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGU4468724.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30879..32639
                     /locus_tag="ACHOQQ_RS02095"
                     /old_locus_tag="pgaptmp_000419"
     CDS             30879..32639
                     /locus_tag="ACHOQQ_RS02095"
                     /old_locus_tag="pgaptmp_000419"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU4468725.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAELRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32764..33048
                     /gene="rplY"
                     /locus_tag="ACHOQQ_RS02100"
                     /old_locus_tag="pgaptmp_000420"
     CDS             32764..33048
                     /gene="rplY"
                     /locus_tag="ACHOQQ_RS02100"
                     /old_locus_tag="pgaptmp_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGU4468726.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33187..34194)
                     /gene="yejK"
                     /locus_tag="ACHOQQ_RS02105"
                     /old_locus_tag="pgaptmp_000421"
     CDS             complement(33187..34194)
                     /gene="yejK"
                     /locus_tag="ACHOQQ_RS02105"
                     /old_locus_tag="pgaptmp_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGU4468727.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34376..34603
                     /locus_tag="ACHOQQ_RS02110"
                     /old_locus_tag="pgaptmp_000422"
     CDS             34376..34603
                     /locus_tag="ACHOQQ_RS02110"
                     /old_locus_tag="pgaptmp_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGU4468728.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34623..36383
                     /gene="yejM"
                     /locus_tag="ACHOQQ_RS02115"
                     /old_locus_tag="pgaptmp_000423"
     CDS             34623..36383
                     /gene="yejM"
                     /locus_tag="ACHOQQ_RS02115"
                     /old_locus_tag="pgaptmp_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGU4468729.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFITFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36458..36534
                     /locus_tag="ACHOQQ_RS02120"
                     /old_locus_tag="pgaptmp_000424"
     tRNA            36458..36534
                     /locus_tag="ACHOQQ_RS02120"
                     /old_locus_tag="pgaptmp_000424"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36492..36494,aa:Pro,seq:ggg)
     gene            complement(36636..39227)
                     /gene="yejO"
                     /locus_tag="ACHOQQ_RS02125"
                     /old_locus_tag="pgaptmp_000425"
     CDS             complement(36636..39227)
                     /gene="yejO"
                     /locus_tag="ACHOQQ_RS02125"
                     /old_locus_tag="pgaptmp_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HGU4468730.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKATTRVMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            39547..40194
                     /gene="narP"
                     /locus_tag="ACHOQQ_RS02130"
                     /old_locus_tag="pgaptmp_000426"
     CDS             39547..40194
                     /gene="narP"
                     /locus_tag="ACHOQQ_RS02130"
                     /old_locus_tag="pgaptmp_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGU4468731.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(40229..41281)
                     /gene="ccmH"
                     /locus_tag="ACHOQQ_RS02135"
                     /old_locus_tag="pgaptmp_000427"
     CDS             complement(40229..41281)
                     /gene="ccmH"
                     /locus_tag="ACHOQQ_RS02135"
                     /old_locus_tag="pgaptmp_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGU4468732.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVLEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(41278..41835)
                     /gene="dsbE"
                     /locus_tag="ACHOQQ_RS02140"
                     /old_locus_tag="pgaptmp_000428"
     CDS             complement(41278..41835)
                     /gene="dsbE"
                     /locus_tag="ACHOQQ_RS02140"
                     /old_locus_tag="pgaptmp_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGU4468733.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT        10253 a        11017 c        10222 g        10403 t
ORIGIN      
        1 ggttaaagga gagggttatg acgaatatca ccttacagaa acaacatcgt acactgtggc
       61 attttattcc ggggttagcc ctgagtgcag ttatcaccgg ggtcgccctg tggggtggtt
      121 ccattcccgc ggttgcgggt gccgggttta gtgccctcac cctcgcaatc ttgttgggga
      181 tggttttagg caacaccatc tatccgcaca tctggaaaag ctgtgacggt ggtgtgctgt
      241 ttgccaagca atatttatta cgtctgggta tcattcttta tggcttccgt ctgacgttct
      301 cgcaaattgc cgatgtcggt atcagtggga tcatcattga cgtgttgacg ctgtccagta
      361 ccttcctgct ggcttgcttc ctggggcaga aagtgtttgg tctggataag cacaccagct
      421 ggttgatcgg tgccggtagc agtatctgtg gtgctgccgc ggtactggcg actgagccgg
      481 tagtgaaagc ggaagccagt aaagtaaccg tggctgttgc aaccgttgtt atcttcggga
      541 ccgtcgcgat tttcctctac ccggcgatat atccgctgat gtctcaatgg tttagtccgg
      601 aaactttcgg tatctacatt ggttctactg tgcacgaagt ggcgcaggtg gtggcggcag
      661 gtcatgccat cagcccggat gcggaaaacg cagcagttat ttccaaaatg ctgcgcgtga
      721 tgatgctggc tccgttcctc atcctgctgg cggcgcgtgt taaacagctg tctggggcga
      781 acagcggcga gaaaagcaaa atcactattc cgtggtttgc catcttgttc atcgtagttg
      841 ccatctttaa ctcgttccac ctgttaccgc agagcgtggt gaacatgctg gtaacgctgg
      901 ataccttcct gctggcaatg gcgatggcgg cgctgggtct gaccactcac gtcagcgcgc
      961 tgaaaaaagc cggggcgaaa cctctgctga tggcactggt actgtttgcc tggctgattg
     1021 ttggtggtgg tgcgattaac tatgtgattc aaagcgtcat cgcataaacc actacatctt
     1081 gctcctgtta acccgctatc attaccgttt tcctccagcg ggtttaacag gagtcctcgc
     1141 atgaaataca ttggagcgca cgttagtgct gctggcggtc tggcaaatgc cgcaattcgc
     1201 gccgccgaaa tcgacgcaac cgcgtttgcc ttgttcacca aaaaccaacg tcagtggcgt
     1261 gccgcaccgc tcacgacgca aaccatcgat gaattcaaag ccgcctgtga aaaatatcac
     1321 tacacatcgg cgcaaattct tccccacgac agctatctga ttaacctcgg acatccggtc
     1381 actgaagctc tggaaaaatc gcgcgatgcc tttatagatg aaatgcagcg ttgcgaacag
     1441 ctggggcttt ctttgctcaa cttccaccct ggcagccatc tgatgcagat ttcagaagag
     1501 gattgccttg cgcgtattgc cgaatccatc aacattgcgc tggataaaac tcaaggtgtg
     1561 accgcggtga ttgaaaacac cgccggtcag ggcagtaact tagggtttaa attcgaacat
     1621 ctcgcggcga ttatcgacgg cgtggaagat aaatcccgtg ttggcgtctg cattgatacc
     1681 tgccatgctt tcgctgccgg gtatgatttg cgtactccag ccgaatgcga gaaaacgttc
     1741 gcggattttg cccgtattgt cggctttaag tatctgcgcg ggatgcacct taacgatgcg
     1801 aaaagcacct ttggcagccg cgttgaccgc catcatagcc tcggtgaagg caatatcggt
     1861 catgatgcgt tccgctggat catgcaggac gaccgcttcg acggcattcc gctgatcctt
     1921 gaaaccatca acccggatat ctgggcagaa gagatcgcct ggctgaaagc gcaacaaact
     1981 gaaaaagcgg tagcctgaag atgaataacc gggaaaagga gatccttgca attttacggc
     2041 gtaacccgct gattcagcag aacgaaattg cggacatgct gcaaatcagt cgttcgcgcg
     2101 ttgcggcgca tattatggat ttaatgcgca aaggccggat taaaggcaaa ggttacattc
     2161 tcaccgagca ggaatactgc gtagtggtgg ggacaatcaa tatggatatt cgcgggatgg
     2221 cggatatccg ttacccgcaa tcggcttctc atcccggtac aattcattgc tcagcgggcg
     2281 gcgtgggacg caacatcgcc cacaatctgg cgctgttagg ccgcgacgtc catttgcttt
     2341 cagtgattgg cgatgacttt tatggcgaaa tgctcctgga agaaacgcgc cgtgccggcg
     2401 tgaatgtctc cggctgcgtt cgtttgcatg gtcaaagcac atcgacgtat ctggcaattg
     2461 ccaatcgaga cgatcaaacc gtgctggcga ttaacgatac ccatctgctg gaacagttga
     2521 caccgcaact actgaacggg tcgcgcgatt tacttcgtca tgcgggcgtg gtactggctg
     2581 attgcaacct gacagccgag gcgctggaat gggtctttac cctcgctgat gagatcccgg
     2641 tgtttgtcga taccgtttca gaattcaaag cgggcaaaat caaacactgg ctggcgcata
     2701 ttcacaccct gaaacccact ttaccggagc tggaaatttt atggggacag gcgatcacca
     2761 gcgatgctga ccgtaatgct gcagtgaatg cgttgcatca gcaaggtgtt cagcaactgt
     2821 ttgtttattt gcccgatgag tcagtttatt gcagcgaaaa ggatggagaa caatttttgc
     2881 tgaccgcacc agcgcatacg acagtagaca gttttggtgc tgacgatggt tttatggcgg
     2941 gcctggtata tagctttctg gagggaaaca atttccgtga cagcgcccgt tttgcgatgg
     3001 cctgcgcggc aatttcacgc gccagcggca gcttaaacaa ccctaccctg tctgccgata
     3061 acgcgctttc attagtgcca atggtgtaac aatgttgccg gattcgacgc ttgaccgcat
     3121 cttatccggc tcataaatcg cacactacgc taaaccaata aagaacccgg caatggtggc
     3181 actcatcagg ttagaaagtg tcgccgccgc cagcgcgcgt aaaccaagct gggcgatttc
     3241 cggcgcacgg tgtggcgcaa ccgcagaaaa cgcccccacc accaccccga tagaaccaaa
     3301 gttagcgaaa ccgcacaacg cgaaggaaat aatcgccacg gtttttgcat cgagagtgcc
     3361 agctgtttgc agatagggtg agaaattgag ataagcgaca aattcattta ttgccagttt
     3421 ctgtccaatc aaactcccgg caagattcgc atcagtccag tcaacaccca ttacccacgc
     3481 cagtggggcc agcaggtaac ctaaaatgga ctccagcgag gcatgttcaa aaccaaacca
     3541 gccgccaacg ccgccgataa taccgttaat taacgcaatg atggcaacaa atgccatcac
     3601 cactgttgcc acacccgcag cgattttcag cccggtcacc gcccctgtcg cggcggcttc
     3661 aataatgctt tttggcggtg tttcggtgaa agagaggtta ttaaaggaaa cctgtgaaga
     3721 ttccgtagcc gggcttagca tgcgggcaaa caagatcccg ccggggatcg ccattaacga
     3781 tgccgccagc aaatattcca caggcacgcc cagtgcggcg taaccaatca tcgtcgaacc
     3841 agcaattgag gccatgccac tacaaatcgc tgtaaataac tcattgcgat tcagccgatc
     3901 gataaagggt ttgacgattg ccggaatttc gttttgcccg aggaaaatgg tagtgaccgc
     3961 gacgaatgac tcgatcttgc tgatatttaa tgctttctgg aatataccgc cgagaatgcg
     4021 aattaagatc cccatcacac cgatgtagta gagaatactc accagcgcgg tgacgaagat
     4081 aattgccggt aacaccctga aaccaaagat aaatcctgcg ccatcaaata aggtgtccat
     4141 tttcggtccg accagagaac cgaagataaa tgcgctaccc gcgtcgctgt acgccatcac
     4201 tttatgcacg ccaaaagcga ctttttcagc gacccaacgc cctggcggta accaaagcat
     4261 aatgccgcca atcacgacct gtaacactaa cgccgcgcca acggtacgca ggctgatctt
     4321 cttcttgttt actgacagta aaaacgcaat cgtcagcaat accaccattc ccagaacact
     4381 tctcatgaca tccatagtta ttatctcttc atgtcatatt gggcgacggc tttctgcccc
     4441 tcagaaatag ccgggcctgc atcttgcgat actgacccgg cttgttatca acacttaatg
     4501 ggttttgatg tagccgcgca cgcactcttc gactaagccc cagaaccagt cggtatcaat
     4561 agtgatgccg actttggtat tagcgggctt gccaagaacg cccagctcgt cgcagacggt
     4621 acgcccatag caagggccac tgttgacgtc cacttcgacg tacatctcct gggttttaat
     4681 gccatcaggg ttaatcagat aaccgatgca ggtggcgtcg tgcaccgggc cgccagccag
     4741 gccgtagttt tcgaactggg ttttgagagt gaagttcatg atgtcgctga acagctctcc
     4801 ggcagggccg cctgcccttt ccatccgagc aattacgtcc ggggtgcaaa cggtctggtt
     4861 ggtaagatcg aggcccatca tcactaatgg aacgccggag gtgaacacca cgcgtgcggc
     4921 ttccgggtca gcaaagatgt tgaactcggc agatggcgtg aagttgcctg taccgtaagc
     4981 gccgcccatt agcacaattt cacggatttt gggcaggatt gcgggttgca tacgcattgc
     5041 caccgcgata tttgacaacg gaccaaccgg caccagagtg atatcgccat cgcttgccat
     5101 cagggtatca atgatgtatt tcaccgcgtg ggtactttct gcctggcggg tcagcggctc
     5161 gaataccggg ccatccagtc cggtttcacc gtgaatatta tcggcaacga tttgttgacg
     5221 cataatgggt tgcggcatcc ccgcataaac cggaacatta atctccagtt tctggcaaac
     5281 attcaggcca ttaattaatg ttttatcaag cgtctgatta cccgcaacaa tggtgatacc
     5341 taataaatca atcgccggat gtttcgccgc catcattata gcaatagcat catcatgacc
     5401 cggatcacaa tccagaataa tttttctctt ttccattgtt tatttcctct gtttccagtt
     5461 gcgttatttt ttctacagca aagaaatttc gatcgggccg atgattgaat cttaacaaca
     5521 gcgtacgtat gctaaatatg agaaatctca tagcggataa acatcgtgaa agaaatccac
     5581 aataatgatc ttaagcagca attgatgagt gaatccgcgt ttaaggattg cttttcaacg
     5641 gatgtttcag ccgatacgcg gctgtttcat tttttagcgc gtgactacat tgtgcaggaa
     5701 gggcaacagc cgtcctggct gttttacctg acgcgaggcc gcgccaggct ttacgccacg
     5761 ctagctaatg gtcgcgtgtc gctgatcgat ttctttgccg ccccctgttt tattggtgag
     5821 attgagttaa tcgataaaga ccatgaaccg cgtgcggtac aggctattga agagtgttgg
     5881 tgccttgcgc tccctatgaa acattaccgt ccgctgttat taaacgacac cctattttta
     5941 cgaaaactct gcgttacttt aagccataaa aattatcgca atattgtttc tttaactcag
     6001 aatcaatcat ttccgttagt gaatcgcctg gcagcattta tattactctc acaagaaggt
     6061 gatctttatc acgaaaagca tacgcaagcg gcagagtatt taggcgtttc ttatcgacat
     6121 cttttatatg ttctcgcgca attcattcac gacggtttat taattaaaag caagaaagga
     6181 tatctaatta aaaacagaaa gcagttgtca gggctggcgc tggagatgga cccggagaat
     6241 aaattctccg ggatgatgca gtaaaaatta tttgcaatag cgcgattgcc ggatgcaacg
     6301 cttaataagc gagtacatcc ggcaatgaaa ctcaagctaa accaataaag aacccggcaa
     6361 tggtcgcgct catcaggttg gaaagcgttg ctgctgccag cgcccgtaaa ccaagctggg
     6421 cgatttccgg agcacgtttt ggcgaaatag ccgaaaatgc cccaacgaca acaccgatag
     6481 aaccaaagtt agcaaaacca caaagcgcaa aggagataat cgcaatggtt ttcacttcca
     6541 gcgtgccgcc cgtttgcagg tagggggaga aactcaggta agcgacgaat tcgttaatcg
     6601 ccagtttctg cccaatcagg ctacccgcaa ggttggcatc actccagtcc acacccatga
     6661 tccacgccag cggtgccagc acatagccaa aaatactttc cagagaagca ttggtaaaac
     6721 caaaccagcc gccaattccg ccgataatgc cgttgatcag cgcaataatt gcgacaaacg
     6781 ccattaccac cgtcgccaca ccggcggcga tttttagccc ggtcatcgca ccgctcgccg
     6841 ccgcttcgat aaagcttttt ggcggcgttt cactgaacga cagattttca aatgtgacct
     6901 gcgaaggctc ggttgccgga ctaagaatac gtgcaaacaa aataccgccg gggatcgcca
     6961 tcagcgatgc cgccaacagg tagtcaattg gtacgcccat tccggcataa ccaatcatca
     7021 tcgaacccgc aatggacgcc atcccgctac aaatagccgt aaacaactcg tttcgattca
     7081 tgcgatcgat aaacggttta acgatcgccg ggatctcatt ttgcccgagg aaaatagtgg
     7141 tgactgcgac aaaagattcg attttgctga tgttgagggc tttctggaaa atgctgccaa
     7201 ggatgcgaat tagcagcccc atcacgccaa tgtagtacag cagactaatg agcgcagtga
     7261 cgaagataat cgccgggagt acgcgaaagg cgaagataaa acccgcaccg tcaaagagga
     7321 catccatttt cggcccgacc agcgaaccaa aaatgaaggc gctaccggca tcactgtaag
     7381 acatcacttt atgaacgcct aatgccgcct gttctactgc ccattttccc ggcggaaaat
     7441 agagcataat gccaccaata gcgatttgca gcagcagtgc ggctccaacc gtgcgcaaac
     7501 tgatgctctt tttatttacc gacaacagaa atgctattgc cagtaacacc accatcccca
     7561 caacacttct cattatatcc ataatgattt tcccttcatg ccagtaaacc cggcgtcagc
     7621 gccaggtttt ggtatgtttg atgagtacgg gcgacggctt tctgcccgtc agaaattaac
     7681 ccgcgagacg ctgatattct ttggcaattt cgctcgccag aatggcgttg ttgaacacca
     7741 gctggatgtt ggatttcagg ctgtcaccgc cggtcagctc agcaacgcgc gccagcagga
     7801 atggcgtact ttctttacca ataacaccct gcgcttcagc ttcagctacc gcctgatcga
     7861 tcgccgcatt gatagtgtgt tccggcatag caaactgttc cgggatcggg ttcgctacca
     7921 cgaggccacc gttcagcccg ctttgccatt tcaccgccat tgcacgggca atttcgctgg
     7981 cgctgtcgag acgaatgctg acgtcaaacg ggctggtgcg gcagaaaaac gcaggcagcg
     8041 cttttgtctg atagccaatt aacggcacac cgaaggtttc taaatactca gtggttaatc
     8101 ctaaatcgag aatagatttc gccccggcac aaacaacggt gacattagta tttgccagtt
     8161 cttgcaaatc ggcagaaata tcgaaggtat gttccgcccc gcgatgcaca ccaccaattc
     8221 ccccggtggc aaatacttta attccggcaa gcgccgcaat aatcatcgtt gaagccacag
     8281 tggttgcgcc attttttccg gcggcaacaa caaaaggtaa atcgcgacga ctaactttgg
     8341 tcacgttatg cccttcacga cccagtaatt caatttcttc tttgcttaaa cccactttca
     8401 tcacgccgcc aataatggcg atagtggcag gtacagcgcc ctgtttacga atagtttctt
     8461 caacttcaat tgcggtctgg gcattttgtg ggaacggcat cccgtgagaa ataatggtcg
     8521 attccagcgc cacaaccggt tttttgtttt ttaaagcgtc ctgcacttcc ggggaaattt
     8581 gtaataattc aggggaaatt tttaattcag acattctgcg ttctccacta acgatataac
     8641 gttggcaatc gataaatcgg ggttattggt gtattcacag gagagcgcca ttgacgaaca
     8701 tccctgtgcg aaacgaacag attcggcaaa cggcattccg tctacccaac acgaagcaag
     8761 tcccgccatc atggcatcgc ccgctccggt aacattaata acattggttt tgatcggcgc
     8821 agaccagcca ctttcaccgc tgatatcgct gtaataaacg ccatcgccgc ccatgctcaa
     8881 taccagtcgg ttcaggccat gttgatggaa ccaggcagca acttttgcca catcttcacg
     8941 ccctgacagc gcaatcccac tcagggtttc cgcttcaagg cggtttggct tgagagtgtg
     9001 gatcagattt agacggtcgc gcactttgac acatttccac gcggaaacag gatcgacaaa
     9061 tacgggtacg ttggcggcat tatccagaat ccatgccagt gcctcttcac tgatattaca
     9121 gtccgcgaca atgacctttg cactctgaat aaattcacgg tgctgtgcaa gatattcagc
     9181 tgtaatagcg ttgctaatat tcatgtcatt tatagcaacc agcatttcac cggtattatc
     9241 gagtaatgat aaataactcg acgtattttc tcccggcaca atcaggcatt tatcgacata
     9301 aacgccagat tgattggttt gcgttagcag cgattgacca taaaaatcac tgcctacggc
     9361 gctcagtagc caggctttgt tacccagcaa cgccaggttt tgtgcaatat tgcgccctac
     9421 tccaccaggc gtaaatttta ttttacctgg atttgaatcc gcataattta atgattcatg
     9481 tgaatatccg gcgacatcaa tattcgccga gcctataatt acgacataat ccttttcgcg
     9541 catagcacgt cctctggtaa aaaattacca accctgtcaa tccacaagaa tatagatact
     9601 gcaaagacgg tttaaacatg tgttcatatt taaacatgtg ctcatagtat gcatgtttta
     9661 cgtaaagtaa attaccgcca gagaggcaaa atgggcagat ttgagcaggc tcacaaaatg
     9721 cgattaaatg cctgaattac aaaagcgagg aatttgtgac gagatgcaca gaacaggaac
     9781 actaaaaaag ggcagaaaag aatctgccct tgaggaaata accttagtat ttgatgaacg
     9841 tttcaggttt ctactgaaag ataaaaccat ttaaattcat aaaattacat tcacaaaccg
     9901 gtcattctct ttgcgctcat ccaatgatac agggcagtaa atttctgccc tgtaacacac
     9961 cttttattac gcggctttcg ctactgcgtc cacttccgga cgtttcagga aggcataggc
    10021 caaactcgcc accagcgtac cggcaataat tgctaccagg taacccagta ccggcgtaat
    10081 agcgccaggg atcagcagaa caaacagacc accgtgcggt gccatcagtt tcgcaccaat
    10141 cgccattgag attgcgccag tcagcgcccc accaacgata caacacggca gcacacgcat
    10201 cggatcacga gcagcaaacg gaattgcacc ttccgaaata aagcacagac caagaaccag
    10261 agcggctttg ccaccttcct gctgcgcttt gtcgaatttg cgacgcgcca ccattgttgc
    10321 cagacccatt gccagcggtg gcaccatacc tgccgccata atcgccgcca tcgggccata
    10381 ggtttgagta ctcagcagac ccacaccgaa tgcgtacgct gctttattta ccggaccgcc
    10441 catgtcagta cacatcatgc caccgaggat cgcccccagc agaaccgcat tcgcagtccc
    10501 catggtctgc agccagtgag tcaacccttc gagaatgcca gcaaccggtt taccgatcag
    10561 gtagatcatc gccagaccga ccaccagact ggaaattagc ggaatgatca ggatcggttt
    10621 cagcgcctcc atactctgtg gcagtttcag ttgcgtactg attaacttcg caatgtaacc
    10681 agccaggaag cccgcaataa taccgccaat gaagccagaa ccggtgctga ccgccagcat
    10741 accgccaatc agacccggcg tgagacccgg acgatcggca atggagaagg caatgtaacc
    10801 tgccagtacc ggcaccatca gcgcaaaggc tgaaccacca ccaatctgca tcagcgccgc
    10861 tgccaacgta cccggctctt taaacgcttc gataccaaaa gcaaaagaaa gcgcgataca
    10921 cagaccacct gcaacgacca tcggcagcat gtaagagacg cccgtcagca agtgacggta
    10981 tgcgcctgca ctctctttct taccttcaga ggtcgccgtt tgagctttgc ccgccggttc
    11041 atacggcgtt gcttcagcaa ccgctttatc cagttcctgc gcggttttct tcagcgccag
    11101 accggtagag gtacgataca tcggtttacc agcaaatttc gccagatcca cttcgatatc
    11161 tgccgccaca atcaccagat ccgctgcggc tacttcttcg ggagtgattg cattacccgc
    11221 accaacagaa ccacgggttt caactttcac ccaccagcca cgttttttcg cttcggtttc
    11281 aatggcttca gccgccataa aggtgtgtgc tacgccagtc gggcaagcag tcaccgcaac
    11341 tacgcgtttc ggaccgctgg cggcaaccgg tgctgtcgca gcgaccggcg cagtgtaagg
    11401 tttcgcatgg cctttggctt cactcaggaa cagctcaggg tgcgcaactg cccgggaaat
    11461 atcgcccagc cagacatttt taccgttcag cgcgctgtca ttcgggatgg aatcaccgag
    11521 aacaatcgcc atttctgcgt cattcggatt gtcgatgatt tccagttttg cttttcgcgc
    11581 cgccgcgccc agcagggtct tcgccatata ggcgcgtgcc tgaccgagat tagcgtcaat
    11641 aatcagcagc gttttcatta tgcctctcct gctgtcagtt aaaaggttgt aagtcgacgc
    11701 gcgccatcat tgcggccaac tgcggacgat cggtaatacc cacattgctt tgacttaccg
    11761 ccagggctgc aacagcagtc gccagacgca gtgtgtgttc actggattca cgcattagca
    11821 agccataaat caggccacca accatagaat cccctgcgcc aacggtgctt acgacatcga
    11881 ctgacggtgg tttggcgatc cattcgccgg aggcattaac ccaaagcgcg ccttcggcac
    11941 ccagtgaaat aacgacatgc gcaatacctt gttcacgcag cgcatgcgca gcttcaatca
    12001 catctttcat ttcaggcagt ttacggcctg cccagatttc cagctcgcgg cggttaggtt
    12061 tcaccagcca cggtgccgct ttcaaacctg ctactaacgc ttcacggcta ctatcaaaga
    12121 taatgcaagg gcactgacta cgcaggcgag tcatccagtc ggtgaacgct tccgggctga
    12181 cgcctgacgg taagcttccg ctgacacaga ccatatcgaa ctgaccgagc cagctcagag
    12241 aatcagtcac aaagcgttcc cagtcggcgg gagtgacttc aaaacccgag aagttgaagt
    12301 cggtcacttc gccgtctttt tccgtcagct taacgttaat tcgggtgcgc ccctgtacaa
    12361 cctggaaacg gttggcaatg cccagctcgc tgaacagttg ctgaaaacca tcctgattgt
    12421 ctttaccgag gaagccgcca acggtgacat caattcccag atcttttaat actttggcca
    12481 cgttgatgcc tttacccgcc gcatgcagac cggtggtttt caccaggttc acttcgccgc
    12541 gttcaatttc cgggcagaaa ccaacaaggt cataagccgg attaagggtg atagtagcaa
    12601 cacgtctgct cattatgcgc cctccccaag accagcagcg atagcgtcgc cgattgcttt
    12661 cagcgcctgt tcagcatctg caccctgggc ggtaaagcgt aggcgatgac ctttcttaac
    12721 gccaagtgcc acaactttca tcagactacg tccgtttgcc ggtttgccgg tgccatcaag
    12781 gtttgtcacg gtaatatcac tgttaaattg tttaatggtg ttgaccagca tggtacctgg
    12841 acgagcatgc aggccgtgtt cattgcgcac cacaaactcc gcgcttaaca cgtcgtcggt
    12901 cggcgcatca tcgctggtca gcagcgccag caacgttgcc gcatccgctt tcagcaagcg
    12961 gtcagcttta ttgtcgagca acaaatcagc gagacgctta agaaccgcaa tgggctgatc
    13021 gtcattcatc gccacactca ccagcatggc tgccgtttcg ccgtccacat caaaagcatt
    13081 tgccgcacgg cttaccgcaa tcgcgctacg caaattgcct tcggcgctat cgctcagcca
    13141 gatgccctgt ccgaggttca gcggttgttc attgatggct ttggtgacga aagtggcgtc
    13201 aactgccccc gcttctttca gacgcgcagc gttcagcgcc tgaagagtca gcagatcgct
    13261 ggcgacgata tccagcgtca gcatttcgtt gtcgagcttc agctgctcac tctgcttttc
    13321 gcccatcagc aatgcgcgaa gttcttctgc tgttgttgct gacttcagtt gttcagcaac
    13381 ggaatcatcg ctcaggacgt gggtcagctg gcgtagcagg cccagatgtt catccgagct
    13441 ggcagcaata ccgattgcca cgtacgctac ctgaccgtca ccccaggtga cgccttccgg
    13501 gaactgaaat acctgaacgc cggttttcag cacctgatcg cgggtgtcgg tagtgccgtg
    13561 tggaatagca ataccattgc cgaggaacgt tgaggtttgc tgttcgcgcg ccagcatgcc
    13621 attgacgtag ccttctgcta cattaccggc ctgcaccagc gccgcagcga cctggcgaat
    13681 cgcctcttct ttgtctccgg ccttttcgcc cggatggatg tcctgtacgg ataactggaa
    13741 catagttctc ctctcttgct gaattgaaac gattcagcct ctatgagaaa aaagcgccaa
    13801 cctagcttag ggttaaagac aagatcgcgc tgaaacgttt caagaaagca taatacttct
    13861 gtttcagcac gcaaggaaag ccgtaaagtt atgagcaaaa gtttgatgtg ctgcacattt
    13921 ttttgtcatt tttcctaatt gctgatggga aaatccgtta tcagcaattt catttcagca
    13981 gcgtcagcag ccaatcagca acagtgaaat ggcattttga ttattcgtgg cgcttttgat
    14041 ttcgctgtct gtttattttc tgacaagcag cgtaaactcc gcgtcttcct cttccagtga
    14101 tcgaccagca tgcataactc ccccgcagtc tccagcgcga aatcgtttga cctgacctcg
    14161 acggcgtttt taatcgttgc ctttctcacc ggtattgcgg gcgctctgca aaccccgaca
    14221 ctcagtattt ttcttaccga tgaagtacat gcccgtccgg cgatggtggg attcttcttt
    14281 accggcagcg ctgtcattgg gattctggtg agtcagtttc tcgccgggcg ctctgataag
    14341 cgcggcgatc gcaaatcgct gattgtcttt tgctgcctgt taggcgtgct ggcctgcacc
    14401 ctttttgcct ggaatcgcaa ctactttgtt ttgctattcg ttggcgtctt tcttagcagc
    14461 tttggctcga ccgctaaccc gcaaatgttt gcccttgccc gtgaacatgc cgacaaaacc
    14521 ggacgtgagg cggtgatgtt cagctctttt ttacgcgctc aggtttcact ggcatgggtc
    14581 attggcccac cgctggctta tgccttagcg atgggtttca gctttacggt aatgtatctg
    14641 agcgcagcgg tagcatttat tgtttgcggc gtgatggtgt ggctgttttt accgtcgatg
    14701 caaaaagagc ttccgctggc gaccggcacg gttgaagcgc cgcgccgtaa ccgtcgcgat
    14761 acgctgctgc tgtttgtcat ttgtacattg atgtggggct cgaacagtct gtacatcatc
    14821 aacatgccgc tatttataat caacgaactg catcttcccg agaaactggc aggcgtaatg
    14881 atggggaccg ccgccgggct ggaaatcccg accatgttga ttgccggata ttttgccaaa
    14941 cgtctgggta agcgtttctt aatgcgcgtt gctgccgtgg gtggcgtctg tttttacgca
    15001 ggaatgctga tggcgcattc acctgccatt ctgttgggct tgcagctgct aaatgctatt
    15061 tttattggca ttctgggcgg catcgggatg ctctattttc aggatttgat gcccggtcag
    15121 gcaggttcag ccaccacgct ctataccaac acgtcgcgcg tgggctggat catcgcagga
    15181 tctgtagcgg gcatcgtcgc cgagatctgg aattatcacg ctgtgttctg gtttgcgatg
    15241 gtgatgatta tcgccactct gttttgctta ctgcggatta aagatgttta aggcgcggtc
    15301 agcatttcca gatcgataag ccaggtcatt gcctgctgac gcgagttacc gcacatttca
    15361 gcgctggctt gtaagcctgc acagactttc ggccgcagtg gcgaggtgaa aattttacag
    15421 cgttgctgtt catcaagctg aatgcagggc gtattggcgg gcttgccatc tggcataccg
    15481 ggaatagggc tggaaattga aggggcagtg caacacgccc cgcaacccgg acggcattcc
    15541 atacgttgtt ctcttaaggt taaccaccgg attgatgagc gcgcacagta ccaccttttt
    15601 tgcaccagca aaagtgcgaa taccacttgc ccgaaaggcc cgtcgcgagt actttgtcgc
    15661 gatatttttg acattttcga ctacaggaat ttttcgatgc caagagcgaa cgaaattaaa
    15721 aaaggtatgg tactgaatta caacggcaaa ctgctgctgg ttaaggacat tgatattcag
    15781 tcgcccactg cccgcggtgc cgctacgctg tacaaaatgc gtttttctga tgtccgtacc
    15841 gggctgaaag tagaagagcg tttcaaagga gatgatatcg ttgacaccgt gacgctgacc
    15901 cgccgttacg ttgacttctc ctatgtcgat ggcaacgaat atgtctttat ggataaagaa
    15961 gactataccc cgtatacctt caccaaagat cagattgaag aagagttgct gtttatgcca
    16021 gagggcggca tgccggacat gcaggtgctg acctgggacg gtcaactgct ggcgcttgag
    16081 ctgccgcaga ccgttgatct ggaaatcgtt gaaaccgcac caggcatcaa aggtgcatct
    16141 gccagcgccc gtaacaaacc ggcgacattg agcactggtc tggtgattca ggtaccagaa
    16201 tacttaagcc cgggcgaaaa aattcgtatc catatcgaag aacgccgtta tatggggcgt
    16261 gctgactaac ttcagccgca tgcagaaaag ggatagctca ggctgtccct tttttaattt
    16321 attataccaa tcttctattt tgcgcttcct gaacacctta cgcccgcaat tttcactcgt
    16381 tgattgatcg ccctcacact tcatcgcatt aacaatccag accaatttca attgctgtca
    16441 tataacttta cactgtcatt gttaattaat cgttactaag acgtgactcc tatgaataca
    16501 atcgcctccg ttacgctccc gcatcatgta cacgctccac gctacgatcg ccagcagttg
    16561 caatcacgta tcgttcattt tggctttgga gcctttcacc gcgcacatca ggcgttactg
    16621 accgatcgtg tgctgaatgc ccagagcggc gactggggga tctgtgaaat cagcttgttc
    16681 agcggtgatc aactgatgag ccagctccgc gcacagaacc atttatacac cgtgctggag
    16741 aaaggggcgg acggcaatca ggcgataatt gtcggtgccg ttcacgaatg cctgaatgca
    16801 aaactggatt ccttagcggc aattattgag aaattttgcg agccacaggt ggcaattgtt
    16861 tccctgacga ttaccgaaaa aggctattgt attgacccgg ccaccggtgc actcgacacc
    16921 agcaatccgc ggattattca cgatctacaa acccctgaag aacctcactc cgcaccgggt
    16981 attctcgtcg aagcactgaa acgccgccgt gagcgcggcc ttacaccgtt taccgtgctc
    17041 tcctgcgaca atattcccga caatggccat gtggtgaaaa acgcggtgct gggaatggca
    17101 gaaaaacgtt cgccagaact cgccgggtgg ataaaagaac acgtcagttt tccgggaacc
    17161 atggtcgacc gcattgttcc ggctgcaacc gacgaatcac tggcagaaat cagccagcat
    17221 ctgggggtaa atgatccctg cgcgattagc tgcgaaccgt ttatccagtg ggtggtggaa
    17281 gataacttcg tcgctgggcg tcctgcctgg gaagtcgcag gtgtacaaat ggtgaatgat
    17341 gtcctgccat gggaagagat gaaactgcgt atgcttaatg gcagccactc ttttctcgct
    17401 tatctgggtt acctctcagg attcgcccat atcagtgatt gcatgcagga tcgcgcattt
    17461 cgccatgctg ccagaacatt aatgctggat gagcaagcgc cgacactgcg gattaaagat
    17521 gtcgatttaa cacaatatgc ggataagtta attgcacgtt ttgctaatcc ggcgctgaaa
    17581 cataagacct ggcaaatcgc gatggatggc agccagaaat taccgcaacg catgctggca
    17641 ggtattcgca tacatctggg gcgcgaaacg gactggtcgt tgctggcatt aggcgttgca
    17701 ggctggatgc gttacgtcag cggcgttgat gatgccggaa atgccattga tgttcgcgat
    17761 ccgcttagcg ataaaattcg cgatcttgtt gcgggcagca gcagtgagca acgcgtcacc
    17821 gccctgcttt ccctgcgtga agttttcggt gatgatctgc cagataaccc gcattttgtg
    17881 caggccatcg aacaagcctg gcaacaaatc gcacaattcg gcgcgcatca ggcgctatta
    17941 aacaccctca aaatttaacg atttctgcgg ttaaagcgga tgaagctcgc cttcgtccgc
    18001 tctccccttc tcttttctgc cgtttttagc caggattaac gctcagttaa cttaccagag
    18061 tattgcggag ccgttgtgac caggaccaac ctcatcaccg gttttctcgg cagcgggaaa
    18121 accacgtcga ttcttcatct gttagcccat aaagatccca acgaaaaatg ggcggtactg
    18181 gttaatgaat ttggggaagt cggaattgat ggtgctttgc tcgccgatag cggcgcactg
    18241 ctgaaagaga tccccggcgg ctgcatgtgc tgcgttaatg gtttacccat gcaggtaggg
    18301 ttgaatacct tactgcgtca gggaaaacca gaccgcttgt tgatagagcc gaccgggctg
    18361 ggccatccga aacagatcct cgatctctta accgcaccag tctatgaacc gtggatagat
    18421 ctacgcgcca cgttgtgcat tctcgatcca cgcctgctgc tggacgaaaa aagcgccagc
    18481 aatgaaaact tccgtgacca gctggctgcc gcagacatca ttgtcgccaa taaatccgac
    18541 cgtgcgacac ctgaaagcga gcaagcgcta cagcgctggt ggcagcaaaa tggtggcgat
    18601 cggcaattaa ttcacagtga gcatgggaaa gttgacggtc atcttctgga tttgccgcgt
    18661 cgcaatttag ccgagttgcc cgccagcgcc gcgcattctc atcagcatgt cgtgaaaaaa
    18721 gggttagcag cgttaagcct gccagagcat caacgctggc gtcgcagtct gaacagtgga
    18781 caaggacatc aggcctgcgg ctggatattc gacgctgata cggtattcga caccattggc
    18841 attctggaat gggcgcgact tgcaccggtg gaacgcgtca aaggcgtgct gcgtattccc
    18901 gaagggctgg tgcgaatcaa ccgtcagggc gatgacctgc acattgaaac gcaaaacgtt
    18961 gcgccaccgg acagccgtat tgagctgatt tccagcagcg aagctgactg gaatggcttg
    19021 cagagcgcgc tgttgaagct tcgtttagcg actaccgcgt aaggttgcct gcgtttttca
    19081 gtaagataat tagagaaaat atgattaaaa atttgccgca aatagtgttg ttgaatattg
    19141 tcggcctagc gctgtttctt tcctggtata ttcccgttaa tcatggattc tggttgccga
    19201 ttgatgcgga tattttttat ttctttaatc agaaactggt cgaaagtaag gcctttttgt
    19261 ggctggttgc attgaccaac aatcgcgctt tcgacggttg ttcactgctg gcgatgggaa
    19321 tgttgatgct gagtttctgg ctgaaagaaa acgccccagg cagacgacgt atcgtgatta
    19381 ttggtctggt catgctatta actgcagtgg tattaaatca gctgggtcag gcattaattc
    19441 ctgtaaaacg ggccagccca acattgactt ttaccgatat taaccgcgtc agcgaactgc
    19501 tctctgtccc cacgaaagat gcctcacgag atagctttcc cggcgatcac ggcatgatgc
    19561 tgcttatttt ttcggcattc atgtggcgtt atttcggcaa agttgcaggt cttatcgccc
    19621 ttattatttt tgtggttttt gcgtttccca gagtaatgat tggcgcacac tggttttctg
    19681 acatcattgt cggttcgatg accgtgatat tgatcggttt gccctgggtg ttgctgacgc
    19741 cattaagtga tcgattaatc accttttttg acaaatcact accgggaaaa aacaaacatt
    19801 tccaaaacaa ataactcaca gtaattaaca tcatcaggtt tatttttata gtgaggataa
    19861 tcctgatgat gcgcaccgtg ctttcatcca tcgaaagcaa aaatcattct ctaagtaaat
    19921 gaatggattg catgcgtttc actcaatcgt actttaattg accaatcccg cttattaact
    19981 ttctgtatca ctttttctta taaaaaatca tgtaaaaccg ctcgccaaga ccgcaccaat
    20041 cgggtaatct cgaactcgtt ttgcctcggc ggtagattat cctcacagca tataattttg
    20101 tgcgttagtc cacagatttg gccttaagga attgtttcaa catgcccagg taattagtct
    20161 cgtgtcgctt ggcatttttt tataacgata tttgtcgtta aggacttcaa gggaaaacaa
    20221 acaacatggt caaatctcaa ccgattttga gatatatctt gcgcgggatc cccgcgattg
    20281 cagtagcggt tctgctttct gcatgtagtg caaataacac cgcaaagaat atgcatcctg
    20341 agacacgtgc agtgggtagt gaaacatcat cactgcaagc ttctcaggat gaatttgaaa
    20401 acctggttcg taatgtcgac gtaaaatcgc gaattatgga tcagtatgct gactggaaag
    20461 gcgtacgtta tcgtctgggc ggcagcacta aaaaaggtat cgattgttct ggtttcgtac
    20521 agcgtacatt ccgtgagcaa tttggcttag aacttccgcg ttcgacttac gaacagcagg
    20581 aaatgggtaa atctgtttcc cgcagtaatt tgcgtacggg tgatttagtt ctgttccgtg
    20641 ccggttcaac gggacgccat gtcggtattt atatcggcaa caatcagttt gtccatgctt
    20701 ccaccagcag tggtgttatt atttccagca tgaatgaacc gtactggaag aagcgttaca
    20761 acgaagcacg ccgggttctc agccgcagct aataaaccgt ttggatgcaa tcccttggct
    20821 atcctgacga gttaactgaa agcactgctt aggcagtgct tttttgtttt cattcatcag
    20881 agaaaatgat gtttccgcgt cttgatccag gctatagtcc ggtcattgtt atcttttaaa
    20941 tgttgtcgta atttcaggaa attaacggaa tcatgttcat acgcgctccc aattctggac
    21001 gtaagctcct gcttacctgc attgttgcag gcgtgatgat tgcgatactg gtgagctgcc
    21061 ttcagttttt ggtggcctgg cataagcacg aagtcaaata cgacacactg attaccgacg
    21121 tacaaaagta tctcgatacc tattttgccg acctgaaatc cactactgac cggcttcagc
    21181 cgctgacctt agatacctgc cagcaggcta accccgaact gaccgcccgc gcggcgttta
    21241 gcatgaatgt ccgtacgttt gtgctggtga aagataaaaa aacattctgt tcatctgcga
    21301 ccggtgagat ggacattcca ctcaatgaat tgattccggc gctcgacatt aataaaaatg
    21361 tcgatatggc gatcttaccc ggcacgccga tggtgccgaa caaacccgca atcgtcatct
    21421 ggtatcgcaa ccctttgctg aaaaatagcg gcgtctttgc cgctctgaat ctcaacctga
    21481 cgccttcact cttttatagt tcacggcagg aagattacga tggcgtcgcc ctcattattg
    21541 gcaatactgc gctatctacc ttttcttcac gtttgatgaa cgttaacgaa ttaaccgaca
    21601 tgccagtccg tgaaactaaa attgcgggca ttcctctgac cgttcggctt tatgcagatg
    21661 actggacatg gaacgatgtg tggtacgcat ttttactggg cggcatgagt ggaactgtcg
    21721 ttggcctgct ctgctattac ctgatgagcg tacgtatgcg ccccggcaga gaaatcatga
    21781 ccgccatcaa gcgcgaacaa ttttacgtgg cgtatcaacc ggtggtggat acacaagctt
    21841 tgcgagtaac gggcctggaa gtactgctac gctggcggca tcctgtcgcg ggagaaattc
    21901 ccccggatgc cttcattaac tttgccgaat cgcaaaagat gattgtgccg ctgactcagc
    21961 acctgtttga gttaattgcc cgcgatgccg cagaattaga aaaagttctg ccggtaggcg
    22021 tcaaatttgg cattaacatt gcgccggacc atctgcacag cgaaagcttt aaagcagata
    22081 tccagaaact gctcacttcc ctacccgcac accatttcca gattgtgctg gaaattaccg
    22141 agcgcgatat gctgaaagag caagaagcca cacaactctt cgcctggctg cactcggtcg
    22201 gcatagaaat tgctattgat gacttcggca ccgggcacag cgcgcttatc tatcttgagc
    22261 gttttacgct cgattatctg aaaattgacc gtggatttat caacgccatc ggtacggaaa
    22321 cgatcacttc ccccgtactt gacgcggtgc tgacgctggc gaaacgcctc aatatgctga
    22381 cggttgctga aggggtcgaa acgccggaac aggcgcgatg gctaagcgaa cgcggcgtta
    22441 atttcatgca aggctactgg attagtcgcc cgttaccgct ggacgatttt gtccgctggc
    22501 tgaagaaacc gtatacgccg cagtggtaag gtgtgcttac gtcccttatt attcatagtg
    22561 aaagcatgcc ggattgcggc taatgatgag taaaaggaaa tccgttgcag atgattgtgc
    22621 gcatactgct gctgtttatc gctctgttca cctttggtgc gcaggcgcag gctatcaagg
    22681 aaagctatgc ctttgccgtg ctgggcgaac cccggtacgc gtttaatttc aaccattttg
    22741 attatgtgaa ccccgccgcg ccaaaaggtg ggcagataac gttgtcagcc ctcggtacct
    22801 tcgataattt caaccgctat gcactgcgcg gcaacccggg cgcacgcacc gagcagctgt
    22861 acgacacgct atttacgact tccgatgacg aaccaggcag ttattacccg ctgattgctg
    22921 aaagcgcacg ctatgctgac gattattcct gggtggaggt cgctattaat ccgcgcgccc
    22981 gttttcatga tggttctccc attactgccc gcgatgtaga gtttactttt caaaaattta
    23041 tgaccgaagg cgtgccgcaa tttcgtctgg tctacaaagg caccaccgtc aaagccattg
    23101 cgccgttaac cgtgcgcatt gagttagcta aacccggcaa agaagatatg ttgagtctgt
    23161 tttcgctgcc ggtatttcca gaaaagtact ggaaggatca caaacttagc gatccgctcg
    23221 ccacgcctcc gcttgccagt ggtccgtacc gaattacgtc ctggaaaatg gggcaaaata
    23281 ttgtctattc ccgcgtgaaa gattactggg cagcaaactt accggtaaac cgtggacgct
    23341 ggaatttcga caccattcgc tacgattatt acctcgatga taatgtcgcc tttgaagcgt
    23401 ttaaagcagg tgcttttgat ttgcgtatgg aaaacgacgc taaaaactgg gccacgcgtt
    23461 ataccggtaa aaatttcgat aaaaaataca tcattaaaga tgagcaaaag aacgaatcag
    23521 cccaggatac gcgctggctg gcgtttaata tccaacgtcc ggtattcagc gatcgccggg
    23581 tccgcgaagc gatcaccctc gcctttgact ttgaatggat gaacaaggcg ttgttttaca
    23641 atgcctggag tcgcacgaac agttattttc agaataccga atacgcggcc agaaattacc
    23701 ccgacgccgc ggagctggtg cttctggcac caatgaaaaa agatctaccg ccagaagtct
    23761 tcacacaaat ctaccagccg ccggtatcca aaggcgatgg ctacgatcgt gacaacctgt
    23821 taaaagccga caaacttctc aacgaagcgg gctgggtgct gaagggtcag caacgcgtta
    23881 atgccacaac gggtcagcca ctcagctttg aattattgct tcctgcaagc agcaatagtc
    23941 agtgggtatt gccgttccag cacagcctgc aacggctggg tatcaacatg gacattcgca
    24001 aggtggataa ctcgcaaatc accaaccgca tgcgcagtcg cgactatgac atgatgccgc
    24061 gcgtatggcg ggcgatgccg tggcccagtt ccgatttaca gatttcctgg tcatcggaat
    24121 atatcaattc cacttataat gcccccggcg tgcaaagccc ggttatcgac tcgctgatca
    24181 accaaattat tgccgcgcag ggaaataaag aaaaattact gccgttgggg cgagcactgg
    24241 atcgcgtatt aacgtggaat tattacatgc tgccaatgtg gtacatggcg gaagaccgtc
    24301 tcgcctggtg ggataaattc tcccaacccg ctgtacgccc tgtttacagc ctgggtatcg
    24361 atacctggtg gtatgacgtt aataaagcgg ccaaactgcc gtcagccaag caacagggag
    24421 agtagatggg cgcttatctg attcgccgtc tgttgctggt gatcccaacg ttatgggcga
    24481 ttatcactat caactttttc atcgtgcaaa ttgcgcctgg cggtccggtc gaccaggcca
    24541 tcgccgccat tgagtttggt aatgccggag tattacccgg cgcaggcggt gaaggcgttc
    24601 gcgccagcca tgcgcaaacg ggcgtcggta atatcagcga cagtaattac cgtggcggac
    24661 gcggattaga tccagaagtg atcgccgaga tcactcatcg ctacggtttc gataagccgc
    24721 tccacgaacg ttacttcaaa atgctctggg actacatccg ctttgatttt ggtgatagcc
    24781 tgtttcgcag cgcctcggtg ctgacgctga ttaaagacag tctgccggtt tccatcaccc
    24841 tcggattatg gagcacgctg attatctatc tggtgtcgat tccgttaggc attcgcaaag
    24901 ctgtttataa tgggagccgc tttgacgtct ggagtagcgc atttatcatc atcggctacg
    24961 ccattccggc ctttttgttt gccatcctgc tgattgtctt cttcgcaggc ggcagctatt
    25021 tcgacctgtt ccctctacgc ggcctggttt ccgctaactt tgattcgctg ccgtggtatc
    25081 agaaaatcac cgattatctg tggcatatca cgctgccggt gctggcgacg gtgattggtg
    25141 gctttgcggc gctgactatg ctgacaaaaa actcattcct tgatgaagtg cgcaagcaat
    25201 acgtggtgac cgcgcgtgcg aaaggggtaa gtgaaaaaaa tattctctgg aaacatgtgt
    25261 tccgcaacgc catgctgctg gtgattgccg gttttccggc gacgtttatc agcatgtttt
    25321 ttaccggctc gctgctgatt gaggtgatgt tttcactcaa tggtcttggc ttactgggct
    25381 acgaagcgac cgtctcgcgc gattatcctg taatgtttgg taccttgtat attttcaccc
    25441 tgattggcct gctgctgaat attgtcagtg atatcagcta tacgctagtc gatccgcgta
    25501 tagattttga gggacgttaa tgtcgcgact cagccccgtc aatcaggccc gttgggcgcg
    25561 ttttcgccat aaccgtcgcg gctactggtc gttatggatt ttcctcgttt tgtttggttt
    25621 gagtttgtgt tctgaactta tagccaacga taaaccgttg ctggtgcgtt atgacggcag
    25681 ttggtatttc ccgttgttga aaaattacag tgaaagcgat tttggcggcc cgctggcaag
    25741 tcaggctgat tatcaggacc cgtggctgaa acaacggctg gaaaataatg gctgggtact
    25801 gtgggcaccg attcgctttg gtgctaccag tatcaacttt gctaccgata agcccttccc
    25861 ttctcccccc tcccggcaaa actggctggg aacggatgcc aacggcggcg atgtgctggc
    25921 acgtattctc tatggcacgc ggatctcggt tctgtttggc ctgatgctga ctctctgttc
    25981 cagcgtgatg ggcgtgctgg cgggggcgct acaaggctat tacggcggta aagtcgatct
    26041 ctgggggcaa cgctttattg aagtatggtc ggggatgccg acgctgtttt tgattatttt
    26101 actttccagc gtcgtacagc ctaacttctg gtggctgctg gcaattactg tcttgtttgg
    26161 ctggatgagt ctggtcggcg tggtgcgggc ggagttttta cgtacccgta atttcgacta
    26221 cattcgtgcg gcgcaggcgc ttggcgtcag cgatcgcagt atcatcctgc gtcatatgtt
    26281 gcctaatgcc atggtcgcga ccctcacctt tttaccgttt attttatgta gttcgatcac
    26341 caccctgacc tcactcgatt tcctcggctt cggtctgccg ctcggttcac cgtcactcgg
    26401 tgaactgctg ttacaaggga aaaataacct tcaggctccg tggcttggga tcaccgcctt
    26461 cttgtcggtg gcgatattat tgtctttgct gatctttatt ggtgaagccg tccgcgacgc
    26521 atttgatcct aataaggcgg tgtagcatga cgcaaactct gttagcgatt gaagatttgt
    26581 cggtgggttt tcgccatcag caaaccgtac gtacagtagt caatgatgtt tcactacaga
    26641 ttgaggctgg cgaaacgctg gcgctggtgg gtgagtcagg ttcaggcaaa agcgttaccg
    26701 cgctgtcaat tttacgcctg ctcccatccc cgccggttga atatctctcc ggcgatattc
    26761 gttttcatgg cgaatcgctg cttcatgcca gcgatcaaac gttgcgcggt gtacgcggta
    26821 ataagatcgc catgattttt caggagccga tggtgtcgtt aaatccattg cataccctgg
    26881 aaaaacagct ttatgaagtg ctttcactcc accgcgggat gcgtcgggaa gcggctcgtg
    26941 gcgaaattct taactgcctt gatcgcgttg gtatccgcca ggcggcaaaa cggctgacag
    27001 attatccgca tcagctctcc ggcggcgaac gccagcgggt gatgattgcg atggcgctgt
    27061 taacgcgacc ggaattatta attgccgatg aaccaaccac cgcactggac gtctctgtcc
    27121 aggcgcagat tttacagctg ttgcgcgaac tgcaaggcga gctgaatatg ggcatgctgt
    27181 ttattactca taacctcagc attgtcagaa aactggccca ccgcgtggcg gtaatgcaaa
    27241 acggtcgctg tgtcgagcaa aataacgccg ctacgctatt tgcctcaccc actcatcctt
    27301 acacacaaaa gctactcaac agtgaaccgt caggcgatcc agtgccgttg ccagaacctg
    27361 cctcaacgtt gctggatgtt gaacagcttc aggttgcctt ccccattcgc aaagggattt
    27421 tgaagcgcat tgtggatcat aatgtggtgg tgaaaaacat cagttttacg ctacgggcgg
    27481 gtgaaacgct gggtttggta ggcgagtctg gttccgggaa aagcacgacg ggactggcgc
    27541 tgctgcgact gattaattct cagggcagca tcatctttga cggtcagcca ctgcaaaatt
    27601 taaatcgccg ccagctgtta cctattcgtc atcgcattca ggtggtattt caggatccaa
    27661 actcctcgct caacccacga ctcaacgttt tgcagattat tgaggaaggc ttacgggttc
    27721 accagccgac gctttctgcc gcacaacgcg aacaacaagt gatagccgtg atgcatgaag
    27781 tgggattaga tcctgaaaca cgccaccgtt atccggcgga gttctctggt ggtcagcgac
    27841 aacgtattgc gattgccagg gcattaattc ttaagccctc gctgatcata cttgatgaac
    27901 cgacatcatc actcgacaaa acggtacagg cgcaaatatt gacgctattg aaatcattgc
    27961 aacaaaagca tcaactggcc tatttgttta tcagccatga tttgcacgtt gtccgcgcgt
    28021 tatgtcatca ggttatcgta ctgcgacaag gggaagtagt ggaacaagga ccgtgcgcgc
    28081 gcgtgtttgc cacaccgcag caggagtata cgcgtcagct actggcgttg agctgacgct
    28141 taaaaaggat tgtagtttga aaagggttcg gcaattgcca caccaaaatt tttcaatcgg
    28201 catgttgcag caaactcgtc ctggcgattt acaaacaggc acggctcacc ttcacactcc
    28261 agcaccgaac atggaacttc gatgtcgctt aacgcctggc ttagtttata catcaccgac
    28321 catgcattat caccatccgg gctaagcaat ttaagcgcca ccaggctgtt gtcaccgcac
    28381 ggtccatttt gcggaatcgg ttcaaccttc gaacctgcga aaccagcaga ccagcgatag
    28441 ttctgcggca gtcgatggac gattgagagt tgtaatgaag tcacttattt tccccggaag
    28501 cacatttact tcacaattta ttcacatcaa ttttaacaca tcatcaacaa tccgtcttta
    28561 tacagatcgt aaagattgat gcctcgcctg cgtggcctct atggctctga tttaagtaat
    28621 atctgcgggc gtggtcacgt ttgcggggct atatgtaccc gtttctgcga tctaactcaa
    28681 cctttttaac tacaatgatg tgacttttta cataaattga ttttacataa aataaacata
    28741 tatcggggga atgatagatt tgtggttgac atgcatcaac aaaggaagcc ttttagcttc
    28801 ctcgttgtgc aatagatcac cgttttttcg gccgactggc gtacagacag aagagaatgg
    28861 agctggttgc gcagaatgca attgaccaaa tcatcggcca tgcagagtta aaggtcgcaa
    28921 gagaaagcaa tgcgccaaca attgccccta tgccaaaacg gaaggttcct gccagcgaag
    28981 atgccgttcc cgccatatgg ggaaactcat caagaatgac cgccatcgca ttggatgaca
    29041 ccatcgacac gcagcccaca aacgccgcaa cgccaaccac cagcgaccag aatcccagcc
    29101 ccagtagcgc actgatgacc atccacgctg ccataataaa ctgtatccac aaccccgagc
    29161 ggaacatatt taacgcgcca atacggcgga cgaagcggct attaaagatg gtcatcacga
    29221 acaggaaaac aatgtttagt gcaaagtaat aaccaaagtt ttccggcgcg acgtggttaa
    29281 tttcaatgta gacaaacggt ccggcgctta agaacgagaa catcccggca aagctgaaac
    29341 cactggccag catataactc aggacacgct tatgacggaa cagcgccgca aagttaccaa
    29401 tagtggtacg aatgtgaaaa ggctggcgac gctccggtgg taaggtttct ttgatcagaa
    29461 agaagatcat tgccgaagcc agaatcgccg ccacggcgag gatccagaag atgtaatgcc
    29521 agctcagcca caccagcacc cagccgccaa ctatcggtgc catcagcggt gcaatggttg
    29581 tcaccagcat gacaaacgac atcatccgcg agaactcttc tttagggtag atatcacgca
    29641 tcaaagcgtt gataaccacg ctggccgcag ccgccgccag cccgtggaag aaacgcatca
    29701 caatcagctg atcgatggtt tgtgccaacg cacacgccac cgcggcggcg gcaaacacca
    29761 gcgtaccgcc aagaaccacc ggcttacgcc cgaagctgtc tgccatcggc ccgtagatta
    29821 actgccccaa cgcaaagccc agaatataag tactgagggt catctgcgta ctgcccgccg
    29881 gtacgccaaa ctgcgctgaa attaccggta gcgcgggcag atacatatca atcgacagcg
    29941 gcatcaacat ggccagcagg ccaaggataa aaacaatagc aaacgacgaa tgctgtcggg
    30001 tggtcacaac gggctcctga aagtcattga aaagttagac gacgctggca atttcttctt
    30061 cagttaacgg acgatattca ccgggggcta aatcagcatc cagcgtaatg ccgccaatac
    30121 gttcacgatg cagctcaacc acatggttac ccacggcggc gaacatgcgt ttcacctgat
    30181 gataacgccc ttcgctgatg gtcagacgaa cctgcgttgg ggtaatcact tccagcaccg
    30241 ccggcttagt gagatctttt tcgttatgca gctgcacgcc tttagcaaat tgctctgccg
    30301 tatcgtcagc tacgggcgat tccagcgtca ccagataggt cttctcgcaa tgatggcgcg
    30361 gagaagtaat gcggtgcgac cactgaccat catctgtcat tagtaccaga ccggtggtat
    30421 cgatatccag ccgccctgcg gcgtgcagtt tccacgccac tggttcatcc agaaaataga
    30481 gcaccgttgg gtgatcaggg tcgtccgtgg agcaaacata gccctgaggc ttattgagca
    30541 tgaagtaacg tggaccgtgt tgctgcgcca gcgggttgcc atcataagcg acatcatgtt
    30601 caggaagcag tttgaacgct gcattacgga cgatttcgcc atcgacggtg acacgattgc
    30661 cgcggatttc acgcccggca atagcacggc taacgccgag ttgctgtgcg ataaatttat
    30721 caagtcgcat gtgtgtgatt ttgcctgtaa aaacggaggt cgggcattga cccgaaaatc
    30781 tgaactgttg tctgcccagt atagcggtct aataacttcc ctcaagggaa aaagaaccgt
    30841 ggcatactat tagcagaata atctacctac gcgagaccat gatttttaca cttcgcccat
    30901 atcagcaaga agccgtggat gccacgctca accattttcg tcgtcataaa acccctgccg
    30961 ttatcgtgct gcccaccggt gcaggtaaaa gcctggtgat agcggaactt gcgcggctgg
    31021 ctcgtggtcg cgtactggtg ctggcacacg ttaaagaact ggtggcgcaa aaccatgcaa
    31081 agtatcaggc gctggggctg gaagccgata tttttgccgc cgggctaaaa cgcaaggaga
    31141 gccacggtaa agtggtattt ggcagcgtgc agtcggtcgc ccgtaatctt gatgcctttc
    31201 agggtgaatt ttcgctgttg attgtcgatg aatgtcaccg tattggtgat gacgaagaga
    31261 gccagtatca gcaaatcctc actcacctga caaaagtgaa tccccactta cgcctgctgg
    31321 ggctgactgc cacgcctttt cgattgggca aaggctggat ctaccagttc cattatcacg
    31381 gcatggtacg cggcgatgag aaagcccttt tccgtgactg catttatgag ctgccgctgc
    31441 gttatatgat taaacacggc tatctgacgc cgccagaacg actggatatg ccagtagtgc
    31501 aatacgattt cagccgcttg caggcacaga gtaacgggct gttcagcgag gccgatctca
    31561 accgcgagct aaaaaaacaa caacgtatta ccccgcacat catcagccag attatggagt
    31621 ttgccgaaaa gcgtaaagga gtgatgattt tcgccgcgac ggtcgaacac gcaaaagaga
    31681 ttgtgggatt actacccgcc gaagatgctg cactgattac tggcgacacc cccggcgctg
    31741 agcgcgatgt gttaattgaa gattttaaag cccagcgttt tcgctatctg gtcaacgtcg
    31801 cagtactgac caccggattt gacgccccgc acgtcgatct tatcgccatt ctgcgcccta
    31861 ccgaatcagt gagtctttac caacaaattg tcgggcgcgg tctgcgtctc gctccgggca
    31921 agactgattg tttaattctt gattatgcgg gtaatcctca cgatctctac gcgccggaag
    31981 ttggtacacc gaaaggcaaa agtgacaacg ttccggtaca ggttttctgc cctgcctgcg
    32041 gttttgccaa caccttttgg gggaaaacga ccgccgacgg gacattgatt gaacactttg
    32101 gtcgccgctg tcagggatgg tttgaagatg acgacggtca tcgcgaacag tgtgacttcc
    32161 gtttccgttt taaaaattgc ccgcaatgta acgcggaaaa cgatattgcc gcccgccgct
    32221 gccgcgaatg tgacaccgta ctggttgatc cagatgatat gttaaaagcg gagctacgac
    32281 tgaaagacgc gctggtatta cgctgtagcg gcatgtcttt acagcatggg cacgacgaga
    32341 aaggcgaatg gttgaaaatc acctattacg atgaagacgg cgcggatgtg agtgagcgtt
    32401 tccgtctgca aacgcccgcc cagcgaactg ccttcgagca gctttttatc cgcccgcata
    32461 cgcgcacacc gggcatccct ctgcgctgga tcaccgccgc cgatatcctc gcccagcaag
    32521 ccttattgcg acacccggat tttgtcgttg cccgcatgaa aggtcagtac tggcaagtgc
    32581 gtgaaaaagt gttcgattac gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat
    32641 ggtagttttg attgatgtat aaggcgattg agtatagaat ctcgcccgct tttgcatacg
    32701 caaagcagat cacttacctg ttgctgggtc gcctgtagca ggattaattt aagagagaaa
    32761 gaaatgttta ctatcaacgc agaagtacgt aaagagcagg gtaagggtgc gagccgccgc
    32821 ctgcgtgccg ctaacaagtt cccggcaatc atctacggtg gcaaagaagc accgctggct
    32881 atcgagctgg atcacgacaa agtcatgaac atgcaagcta aagctgaatt ctacagcgaa
    32941 gttctgacca tcgttgttga cggtaaagaa atcaaagtta aagctcagga cgtacagcgt
    33001 cacccgtaca aaccgaagct gcagcacatc gacttcgttc gcgcttaatt gctgaataag
    33061 ttgtaaaaaa ccccgctccg gcggggtttt ttgtatctgc agattatgcc tgattacgat
    33121 attgctattt tttgcaggcc agataaggcg ttcacgccgc atctggcatt cataacacaa
    33181 aaccgattaa ttgccgccgg acgtccggcg ctgcaattga tcgcgcaaat tcggcggcgt
    33241 gcctttaata gtcagggtat cggtcgccgg atcccagaaa atccgttcgc ccaacagcat
    33301 cgcatcaaaa ttaatagtca agccaccgcc gctaccggca aacttcgtca actgacgcag
    33361 tgtgctacgg tctgccggga agctctcttc cagttcgtag cctttttcag ccgcaaactc
    33421 cgtgaagctc acttcgctga cgcctgccag ctctttcgac agcgattcca gttcgatctc
    33481 ttcgcctgcc tgcagctgct cattacagta gctataaacc tgctggcgca cgttctgtcg
    33541 ctctgcttta tccagttgtg cttcggcagt gaaatcatca accgcttgca acagcccacg
    33601 gttttgcgct ttcgcgttca atccttcgct ggccccgagg aaatccataa agaaatccgc
    33661 cactttgcgc cctactctcc ctttcaggaa agtgagatag cgggtcgact ctggattggt
    33721 ttcccattcg gttaaatcga tacgcgcaac aatatccgca tgattgatat caagataatg
    33781 ggttgggttg atgtcgagat tttcgttaac gcgcatactg ctcaggttgc tcagaactgc
    33841 caccagcaaa tactccaccg ccagatagcg atagtggcaa aacagcacga atccaccgtc
    33901 agcaaaagga tatttcgcta attcgtcacg caggcgaccg gttgccgcgc ggctaaatgc
    33961 cagaaagtcc tcttcaccct gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc
    34021 gctaaacaag ccgtaagctt tatttttggc gctatagacc cgatgcagtt ctgctaccat
    34081 ctcaacgacg gtttctgtcg gttccagcaa tgaatcgcgc aacaccagct caaggttctg
    34141 ctcatcacgc ttgataagct ggtgcagagc aatctggttg atatccagac tcatgataaa
    34201 ctctccttta agaccgggcg gtattcaacc accgccggtt aagcgacgca ataaaagatg
    34261 gcggtcattt tacagaaggc ggcgtaacga gaatgattta acgcataaaa aagcagaaaa
    34321 aaaaccgttg ctacggtaat atgttgccct ttcatcaact aactgatttc gatttatgcc
    34381 acaaatttcc cgctacagtg atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt
    34441 actggaaaaa cataaggctc cgaccgacct ttccctgatg gtgttaggca acatggtcac
    34501 taaccttatc aacaccagca ttgccccggc ccaacgccag gcaattgcca actcttttgc
    34561 ccgcgcctta cagtcctcta tcaacgaaga caaagcgcac taagggaaac agataacagg
    34621 ttatggtaac tcatcgtcag cgctaccgtg aaaaagtctc ccagatggtc agttgggggc
    34681 actggtttgc actgttcaat attctgcttt cgctcgtcat tggcagccgt tacctgttta
    34741 tcgccgactg gccgaccacg cttgctggtc gcatttattc ctacgtaagc attatcggcc
    34801 atttcagctt cctggtgttc gccacctact tgctgatcct cttcccgctg acctttatcg
    34861 tcggctccca gaggctgatg aggtttttgt ccgtcattct ggcaacggcg ggaatgacgc
    34921 tattactgat cgatagcgaa gtctttactc gtttccatct ccatcttaat cccatcgtct
    34981 ggcaactggt tatcaaccca gacgaaaatg agatggcgcg cgactggcag ctgatgttca
    35041 tcagcgtgcc ggttatttta ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc
    35101 tgcgcagcct gacgcgtcgt cggcgcttcg cgcgcccgct ggccgcattc ttatttatca
    35161 cctttatcgc ctcgcatgtg gtgtatatct gggccgatgc caacttctat cgccctatca
    35221 ccatgcagcg cgctaacctg ccgctttcgt acccgatgac ggcgcgacgt tttcttgaga
    35281 agcatggcct gcttgatgcg caggagtatc aacgccgtct tattgagcaa ggtaatccag
    35341 acgccgtttc cgttcagtat ccgttaagcg aactgcgcta tcgcgatatg ggcactgggc
    35401 agaatgtgct gttgattact gtcgatggcc tgaactactc acgcttcgag aagcagatgc
    35461 ctgcgctggc aggttttgct gagcaaaata tttcgttcac gcgccatatg agctccggca
    35521 acactacaga caacggcatc tttggcctgt tctatggcat ctcgccgagc tatatggacg
    35581 gcattctgtc gacccgtacg cctgcggcat taattactgc gcttaatcag caaggctatc
    35641 agctggggtt attctcatca gatggcttta ccagcccgct gtatcgccag gcattgttgt
    35701 cagatttctc gatgccgagc gtacgcaccc aatccgacga gcagaccgcc acgcagtgga
    35761 tcaactggct gggccgctac gcacaagaag ataaccgctg gttctcgtgg gtctctttca
    35821 atggcactaa cattgacgac agcaatcagc aggcatttgc acggaaatat agccgggcgg
    35881 caggcaatgt cgatgaccag atcaaccgcg tgctcaatgc actgcgtgat tctggcaaac
    35941 tggacaacac ggtggtgatt atcactgccg gtcggggtat tccactgagc gaagaggaag
    36001 aaacctttga ctggtcccac ggtcatctgc aggtgccatt agtgattcac tggccaggca
    36061 cgccggcgca gcgtattaat gcgctgactg atcataccga tctgatgacg acgctgatgc
    36121 aacgcctgct acatgtcagc acacctgcca gcgaatattc gcaaggtcag gatttgttca
    36181 accctcaacg ccgtcattac tgggttaccg cagcggataa cgatacgctg gcaattacca
    36241 ccccgaaaaa gacgctggtg ctgaacaata acggtaaata ccgcacttac aacttacgtg
    36301 gtgaaagagt gaaagatgaa aaaccacagt taagtttgtt attgcaagta ctgacagacg
    36361 agaagcgttt tatcgctaac tgattaatta taaatcagtt agcgaaatat cttacttgca
    36421 atcggtgtgg aaaacggtag tattaacagc cacgagtcgg cacgtagcgc agcctggtag
    36481 cgcaccgtca tggggtgtcg ggggtcggag gttcaaatcc tctcgtgccg accaaaaatc
    36541 ccaaaaaaaa ccaaccctta cggttggttt ttttatatct gcaattaatt cgataaacag
    36601 accgtgacac atcacagcct gtttattttc tgttatcaga acgtccagac cacacccgcc
    36661 tgagtattcc acggcgattc tacccctgcg ccattgccat agctgactga caaatgaccg
    36721 cttaacgtcg gggtaaacga tgaccttatc ccagcctgat aaacgccact ggttccagcc
    36781 acatcattat taaatttgcc atcgtcatta actttcacct ggttagaatc ggcgtattcc
    36841 tgacgcacgg cggctttcag ccagggttcc agcgtcgtac cgttttgcag gtccatgtga
    36901 tagcttaccg ccgttcccgc ttcagcgcgt aatatccggg tatttcccac gtccgcgcgc
    36961 atgccgtttg ataacgtgta gtcctgacca tctgtggtaa agccggtaaa ggccagatag
    37021 ggtctaacac tccacaatcc gtcaacccaa cggaacccgc tctcaacatg agcacccgcg
    37081 ccgttactat tgtaatcgcc aaacgctgtt gccccattac tcatcttgcc atggatggtg
    37141 ttggcaaaac ggtcaacttt caccacccca tcaacatagg caccgttctg atgctcccaa
    37201 ccggcataag cccccagggt atagctatcg atattacctt ttccgccgcg atcaaaacca
    37261 atatcagaat gagagtaacc aaagaacaag ccgcgaattg tactgctttc ttcacgggag
    37321 aaacggctat cgataccgag cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca
    37381 tcagtggtca cgttgttgcg ggtgttaatt gccgaactcc acatcgccgt atcgtaacta
    37441 acgcctttta cgctaccaag acgctcacgc acggtgtcca gttctgcatc aaataccagc
    37501 ggttgtgctg ccgccatatt tagcacatca gtggttgaag gggtaatttg cgcgcgattc
    37561 tctgccagac tccagctatg gttaccatta tccagcaagg tatattcata cgtaccgata
    37621 tcaacaacgc ctccggcatt gcccaacgta aatgcagcat caccgccgcc cgttgttacc
    37681 agtgtaaggc tatctcctgc tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa
    37741 tcacctgttg cctgaccggt gacgttgagc tgatcgctct gatgattagc catatcggta
    37801 cgcatataaa aattgccgtt tcccgacagg gtattggtgg tcaacgtaat gaaattttcc
    37861 gcacttgccg tcactgatga acgagtcact ggctcatcca tcataatcac atttgcgtca
    37921 gacatcgcaa ggttggctaa gttgaggagg gtgttttggt tcgtcgtagt gatatcggca
    37981 atgatggtcc atacgctatt ttcgagcgaa acgtccgctt tgctggtatc cacggcgcca
    38041 tgcgttctaa aagaagcgcc ctcagaaacc actaccttac ccaccaacat tgcgggtgca
    38101 taatttattt gcggcttcat gacctcaaga atgccatcat tccctctgac tgaaacgtta
    38161 accattgtgc cagcccagac gttagcgcgg ccattgttga taaccatatt ttcagagatg
    38221 gcttttgagg aatacgtgat acttccattc tgatctgatc gaccgagatc atataccgca
    38281 tttgcctgca ctctcgtccc agtatccttt cccagcgatt gcatagtggc atgctcatca
    38341 aggatagtgt caatggcctg cgtgtcctcc ataacaataa gcccggaacc atcatccagt
    38401 tcataatttt ttgacacacc atcttttata ctaaattggc ctttactgtt tgtaccactc
    38461 acttccagcg cattcgttga gacaattaat tttccgcctg cttttttatc aacgccagtt
    38521 gcagtcccgc catccataac ctccagtaag ccgccactat ctacagtagt attataagcg
    38581 aagtcatttt cttcaactcg caaacttccg ccgttttcca acagcatatt gtttgcaatt
    38641 ccattcttga tatcgaactg accgagacgg tttgttccga atacctccat gacccgcgtg
    38701 gttgctttaa tagcaccgcc tgctttctga tctaccgcaa tcgctaatcc cccctcttta
    38761 acctccagta atcctccctt ttcgatagcg gcaaggacgg taatcccatc tttttcaacg
    38821 attaagcgcc cgcctttaat cgtgggatca ttcgccgcgc cataaacctg tagcacacca
    38881 ttattgaggg tagtctggtt tgccgcgcca cccatttgaa caatttgata accatcattt
    38941 aatacggagt attctgcggt gccattcatt tcaatgtact gatacccgcc gttaatttca
    39001 gtattactac ttataccaaa ttctttaata tgctgttctc cgccaggatt gattttcgta
    39061 ttattggcgg tcccataaac cagctggata tctttttcca ggacaacacc atcaactgtc
    39121 tcaccatact caactgttga tgccaatgct atgggtgaat ataacgctgt agccaccaga
    39181 acagatattg cggaacgaca aagagaaaca gaaccagatt gatgcattga gctttcatcc
    39241 tatgaaatta attgctgtta aaagcattgg gtacagaaaa tacccatagc tccatacccg
    39301 gaatcagttt ttaaaaactg tttaaagaaa tgcacaagta ttgtgattga ttttttagtt
    39361 gtttttcttg atgagaagct gatgcaaaat tccgtcttta taatgaaaat gatgccaaag
    39421 cgaacggcaa ggttgtagtt ttcactacat gtccatacat aaaatggggt aacattcacg
    39481 cgcctggtag cgttaccaac gctacgctca aacataatga ttctaataaa acctcaggag
    39541 actactatgc ctgaagcaac gccttttcag gtgatgattg tggatgatca tccacttatg
    39601 cgacgcggtg ttcgtcagtt actggagctt gatcctggct ttgaagtggt cgccgaagcg
    39661 ggcgacggcg cgagcgcgat cgatctggcg aatagactgg atatcgacgt gatcttgctg
    39721 gatctgaata tgaaaggtat gagtggcctg gataccctca atgccttgcg cagggatggc
    39781 gttaccgcgc aaattattat cttgaccgta tcagatgcct ccagcgatgt ctttgcactg
    39841 atagacgcag gcgcagacgg ttatctgttg aaagacagcg acccggaagt gttgctggaa
    39901 gcgattcgtg ccggagcgaa aggcagcaaa gtctttagcg aacgcgtcaa tcagtactta
    39961 catgaacgtg aaatgtttgg cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag
    40021 ctggatgttc tgcacgagct ggcacagggg ctgtcaaata aacagattgc ctcggtgttg
    40081 aatatttcag agcagacagt aaaagtacat attcgcaatc tgctgcgcaa actcaatgtc
    40141 cgctcacgcg tggcagccac cattctgttc ctgcaacaac gcggggcaca ataaaaatag
    40201 cccgatggca tcaccatcgg gcctcttttt atttactctc ctgcggcgac aaatgttgca
    40261 tcgcctgcgc gatactacgt tcaatcaccg cacggcgagt atcgttggca ggtaagagtt
    40321 tcaacatcat ctcccacgcg gcaacggctt cgccaaatcg ctgctgctca aaggcattaa
    40381 acgcatacat gcttaacaca cgaatattgc tatggtccgt tctcaccaac tggcgcagca
    40441 gttcgccgcc gaggcggttg tcgttgggat cagatgaacg tgtcaacgct tcagcataac
    40501 ccagtgcggc atcgctgttt ttcggatcga ggcgatacgc agtagcgtat gcatcggtgg
    40561 cgatactggc gttacccagc gccatgccaa cgcgacccaa cataatccaa ccttctatat
    40621 ctcccggatt tttttgcagt tgagtacgca tccccagcgc aaggcgcgac atctcttctt
    40681 cgttgagcgg atcggctttc ggatccagcg ccctgtccag caacgccgga gcctgtgccg
    40741 tggcctgctg ccagattttc acctgctgat aattgccagt ctggtagtag ctgacgccag
    40801 ccacaattaa cgccaccaca atacccggca gataaacaac atatccggca cgcttacctt
    40861 ccggcacgct ttgttcagga aacgcttcca gcaccacgcg tacccgacgc cgcgagcggg
    40921 cgtaaatgac ccaaccgcca atgccaatag ccactactgg cagcacccac agcagcacgg
    40981 tcagcggcgt taacggcgga tcgtaagtga cgaagttgcc gtaacgcgcc accatataat
    41041 cgacaatctc tttcttactt ttaccttcct gcatcagttc atacactttc tggcgcaggt
    41101 cggtggcaat catcgagttg gaatcggcaa tgctgttatt ctgacatttc gggcagcgca
    41161 gttcttcagt gagctgacgg aactgttgtt cctgtgcttc atctttaaac tgcaacacgt
    41221 cgatggtcgc cagcgctgag ccggagatca tcagcatcag cacgcccaat aaaaacctca
    41281 ttgtgcggcc tccttactgt atttctccca cagcggcttg atctcttctt cccagacgcg
    41341 aggattcaga tcgcccgcat ggcgatagcg aatgatgccg ttgccgtcaa taaggaacgt
    41401 ttctggcgcg ccatagacac cgagatccag ccctaacatg ccgtcgccat caaacaggct
    41461 tagcgcgtaa ggattgccca gctctttcag ccagctgatt gccttctggc gatcgtcttt
    41521 atagttcatg ccgaccacgc ggatgccctg cgcagaaagc tgattcagat attgatgttc
    41581 cgcacggcag gtcggacacc aggtcgccca gacgttaagc agcactggtt tgccctgagt
    41641 cagcacatcc gcctgataaa actgccccgg attgtccagt gattcgaggc gaaacttcgg
    41701 cacaggcttg ccaatgagcg ccgattccag attggtcgga tcatcccctt cggcattacg
    41761 cgccaactgc cacagcagcg ccgcggcaat cgccaggaag ataatcaaag gaattaacaa
    41821 tactttgcgc ttcatacagc ctccggcgca gttttctgcg ggttcacgcg cttacgatag
    41881 cgaggatcaa acagg
//
LOCUS       DAZKFU020000010        40466 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZKFU020000010 DAZKFU020000000
VERSION     DAZKFU020000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.010"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            287..373
                     /gene="leuL"
                     /locus_tag="ACHOQQ_RS02145"
                     /old_locus_tag="pgaptmp_000429"
     CDS             287..373
                     /gene="leuL"
                     /locus_tag="ACHOQQ_RS02145"
                     /old_locus_tag="pgaptmp_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGU4468734.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            466..2037
                     /gene="leuA"
                     /locus_tag="ACHOQQ_RS02150"
                     /old_locus_tag="pgaptmp_000430"
     CDS             466..2037
                     /gene="leuA"
                     /locus_tag="ACHOQQ_RS02150"
                     /old_locus_tag="pgaptmp_000430"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGU4468735.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2037..3128
                     /gene="leuB"
                     /locus_tag="ACHOQQ_RS02155"
                     /old_locus_tag="pgaptmp_000431"
     CDS             2037..3128
                     /gene="leuB"
                     /locus_tag="ACHOQQ_RS02155"
                     /old_locus_tag="pgaptmp_000431"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGU4468736.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYY
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASTIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3131..4531
                     /gene="leuC"
                     /locus_tag="ACHOQQ_RS02160"
                     /old_locus_tag="pgaptmp_000432"
     CDS             3131..4531
                     /gene="leuC"
                     /locus_tag="ACHOQQ_RS02160"
                     /old_locus_tag="pgaptmp_000432"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGU4468737.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4542..5147
                     /gene="leuD"
                     /locus_tag="ACHOQQ_RS02165"
                     /old_locus_tag="pgaptmp_000433"
     CDS             4542..5147
                     /gene="leuD"
                     /locus_tag="ACHOQQ_RS02165"
                     /old_locus_tag="pgaptmp_000433"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGU4468738.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5255..5386)
                     /gene="sgrT"
                     /locus_tag="ACHOQQ_RS02170"
                     /old_locus_tag="pgaptmp_000434"
     CDS             complement(5255..5386)
                     /gene="sgrT"
                     /locus_tag="ACHOQQ_RS02170"
                     /old_locus_tag="pgaptmp_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGU4468739.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5475..7133
                     /gene="sgrR"
                     /locus_tag="ACHOQQ_RS02175"
                     /old_locus_tag="pgaptmp_000435"
     CDS             5475..7133
                     /gene="sgrR"
                     /locus_tag="ACHOQQ_RS02175"
                     /old_locus_tag="pgaptmp_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGU4468740.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIARIMQQILASHQVTMEIKEISYDQWHKGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPLDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7362..7931)
                     /gene="espY2"
                     /locus_tag="ACHOQQ_RS02180"
                     /old_locus_tag="pgaptmp_000436"
     CDS             complement(7362..7931)
                     /gene="espY2"
                     /locus_tag="ACHOQQ_RS02180"
                     /old_locus_tag="pgaptmp_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HGU4468741.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDQLTHWLIK
                     FNELKEHARDPENMAAKASLSPKDTLCVSFFIGDEAIFTLKLQLKENTRTGCIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8173..8272
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8303..9286
                     /gene="thiB"
                     /locus_tag="ACHOQQ_RS02185"
                     /old_locus_tag="pgaptmp_000437"
     CDS             8303..9286
                     /gene="thiB"
                     /locus_tag="ACHOQQ_RS02185"
                     /old_locus_tag="pgaptmp_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGU4468742.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9262..10872
                     /gene="thiP"
                     /locus_tag="ACHOQQ_RS02190"
                     /old_locus_tag="pgaptmp_000438"
     CDS             9262..10872
                     /gene="thiP"
                     /locus_tag="ACHOQQ_RS02190"
                     /old_locus_tag="pgaptmp_000438"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGU4468743.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVIAVALAAFLALWSNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            10856..11554
                     /gene="thiQ"
                     /locus_tag="ACHOQQ_RS02195"
                     /old_locus_tag="pgaptmp_000439"
     CDS             10856..11554
                     /gene="thiQ"
                     /locus_tag="ACHOQQ_RS02195"
                     /old_locus_tag="pgaptmp_000439"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGU4468744.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSIVVADGRIAWQGKTEELL
                     SGKASASALLGITG"
     gene            complement(11641..12405)
                     /gene="yabI"
                     /locus_tag="ACHOQQ_RS02200"
                     /old_locus_tag="pgaptmp_000440"
     CDS             complement(11641..12405)
                     /gene="yabI"
                     /locus_tag="ACHOQQ_RS02200"
                     /old_locus_tag="pgaptmp_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4468745.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12491..13369)
                     /gene="araC"
                     /locus_tag="ACHOQQ_RS02205"
                     /old_locus_tag="pgaptmp_000441"
     CDS             complement(12491..13369)
                     /gene="araC"
                     /locus_tag="ACHOQQ_RS02205"
                     /old_locus_tag="pgaptmp_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGU4468746.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13708..15408
                     /gene="araB"
                     /locus_tag="ACHOQQ_RS02210"
                     /old_locus_tag="pgaptmp_000442"
     CDS             13708..15408
                     /gene="araB"
                     /locus_tag="ACHOQQ_RS02210"
                     /old_locus_tag="pgaptmp_000442"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGU4468747.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHMP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15419..16921
                     /gene="araA"
                     /locus_tag="ACHOQQ_RS02215"
                     /old_locus_tag="pgaptmp_000443"
     CDS             15419..16921
                     /gene="araA"
                     /locus_tag="ACHOQQ_RS02215"
                     /old_locus_tag="pgaptmp_000443"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGU4468748.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17090..17785
                     /gene="araD"
                     /locus_tag="ACHOQQ_RS02220"
                     /old_locus_tag="pgaptmp_000444"
     CDS             17090..17785
                     /gene="araD"
                     /locus_tag="ACHOQQ_RS02220"
                     /old_locus_tag="pgaptmp_000444"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4468749.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17860..20211
                     /gene="polB"
                     /locus_tag="ACHOQQ_RS02225"
                     /old_locus_tag="pgaptmp_000445"
     CDS             17860..20211
                     /gene="polB"
                     /locus_tag="ACHOQQ_RS02225"
                     /old_locus_tag="pgaptmp_000445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGU4468750.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGNASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20375..23281
                     /gene="rapA"
                     /locus_tag="ACHOQQ_RS02230"
                     /old_locus_tag="pgaptmp_000446"
     CDS             20375..23281
                     /gene="rapA"
                     /locus_tag="ACHOQQ_RS02230"
                     /old_locus_tag="pgaptmp_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGU4468751.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISVLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTDSKLVNAVQQDVHAILQLGEAQIEKSARALIDTARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23293..23952
                     /gene="rluA"
                     /locus_tag="ACHOQQ_RS02235"
                     /old_locus_tag="pgaptmp_000447"
     CDS             23293..23952
                     /gene="rluA"
                     /locus_tag="ACHOQQ_RS02235"
                     /old_locus_tag="pgaptmp_000447"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGU4468752.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24070..24885)
                     /gene="djlA"
                     /locus_tag="ACHOQQ_RS02240"
                     /old_locus_tag="pgaptmp_000448"
     CDS             complement(24070..24885)
                     /gene="djlA"
                     /locus_tag="ACHOQQ_RS02240"
                     /old_locus_tag="pgaptmp_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGU4468753.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGDNWQQTQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25140..27494
                     /gene="lptD"
                     /locus_tag="ACHOQQ_RS02245"
                     /old_locus_tag="pgaptmp_000449"
     CDS             25140..27494
                     /gene="lptD"
                     /locus_tag="ACHOQQ_RS02245"
                     /old_locus_tag="pgaptmp_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGU4468754.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27547..28833
                     /gene="surA"
                     /locus_tag="ACHOQQ_RS02250"
                     /old_locus_tag="pgaptmp_000450"
     CDS             27547..28833
                     /gene="surA"
                     /locus_tag="ACHOQQ_RS02250"
                     /old_locus_tag="pgaptmp_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGU4468755.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            28833..29822
                     /gene="pdxA"
                     /locus_tag="ACHOQQ_RS02255"
                     /old_locus_tag="pgaptmp_000451"
     CDS             28833..29822
                     /gene="pdxA"
                     /locus_tag="ACHOQQ_RS02255"
                     /old_locus_tag="pgaptmp_000451"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGU4468756.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAILGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLSGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            29819..30640
                     /gene="rsmA"
                     /locus_tag="ACHOQQ_RS02260"
                     /old_locus_tag="pgaptmp_000452"
     CDS             29819..30640
                     /gene="rsmA"
                     /locus_tag="ACHOQQ_RS02260"
                     /old_locus_tag="pgaptmp_000452"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGU4468757.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30643..31020
                     /gene="apaG"
                     /locus_tag="ACHOQQ_RS02265"
                     /old_locus_tag="pgaptmp_000453"
     CDS             30643..31020
                     /gene="apaG"
                     /locus_tag="ACHOQQ_RS02265"
                     /old_locus_tag="pgaptmp_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGU4468758.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31027..31875
                     /gene="apaH"
                     /locus_tag="ACHOQQ_RS02270"
                     /old_locus_tag="pgaptmp_000454"
     CDS             31027..31875
                     /gene="apaH"
                     /locus_tag="ACHOQQ_RS02270"
                     /old_locus_tag="pgaptmp_000454"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGU4468759.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(31872..32186)
                     /locus_tag="ACHOQQ_RS02275"
                     /old_locus_tag="pgaptmp_000455"
     CDS             complement(31872..32186)
                     /locus_tag="ACHOQQ_RS02275"
                     /old_locus_tag="pgaptmp_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HGU4468760.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(32189..32422)
                     /locus_tag="ACHOQQ_RS02280"
                     /old_locus_tag="pgaptmp_000456"
     CDS             complement(32189..32422)
                     /locus_tag="ACHOQQ_RS02280"
                     /old_locus_tag="pgaptmp_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HGU4468761.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(32508..32987)
                     /gene="folA"
                     /locus_tag="ACHOQQ_RS02285"
                     /old_locus_tag="pgaptmp_000457"
     CDS             complement(32508..32987)
                     /gene="folA"
                     /locus_tag="ACHOQQ_RS02285"
                     /old_locus_tag="pgaptmp_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGU4468762.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLAKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33179..35041)
                     /gene="kefC"
                     /locus_tag="ACHOQQ_RS02290"
                     /old_locus_tag="pgaptmp_000458"
     CDS             complement(33179..35041)
                     /gene="kefC"
                     /locus_tag="ACHOQQ_RS02290"
                     /old_locus_tag="pgaptmp_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGU4468763.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(35034..35564)
                     /gene="kefF"
                     /locus_tag="ACHOQQ_RS02295"
                     /old_locus_tag="pgaptmp_000459"
     CDS             complement(35034..35564)
                     /gene="kefF"
                     /locus_tag="ACHOQQ_RS02295"
                     /old_locus_tag="pgaptmp_000459"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGU4468764.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLDGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGKSHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35675..37006)
                     /gene="yaaU"
                     /locus_tag="ACHOQQ_RS02300"
                     /old_locus_tag="pgaptmp_000460"
     CDS             complement(35675..37006)
                     /gene="yaaU"
                     /locus_tag="ACHOQQ_RS02300"
                     /old_locus_tag="pgaptmp_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4468765.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(37064..37351)
                     /gene="fixX"
                     /locus_tag="ACHOQQ_RS02305"
                     /old_locus_tag="pgaptmp_000461"
     CDS             complement(37064..37351)
                     /gene="fixX"
                     /locus_tag="ACHOQQ_RS02305"
                     /old_locus_tag="pgaptmp_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGU4468766.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37348..38634)
                     /gene="fixC"
                     /locus_tag="ACHOQQ_RS02310"
                     /old_locus_tag="pgaptmp_000462"
     CDS             complement(37348..38634)
                     /gene="fixC"
                     /locus_tag="ACHOQQ_RS02310"
                     /old_locus_tag="pgaptmp_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4468767.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38685..39626)
                     /gene="fixB"
                     /locus_tag="ACHOQQ_RS02315"
                     /old_locus_tag="pgaptmp_000463"
     CDS             complement(38685..39626)
                     /gene="fixB"
                     /locus_tag="ACHOQQ_RS02315"
                     /old_locus_tag="pgaptmp_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4468768.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGTDGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39641..40411)
                     /gene="fixA"
                     /locus_tag="ACHOQQ_RS02320"
                     /old_locus_tag="pgaptmp_000464"
     CDS             complement(39641..40411)
                     /gene="fixA"
                     /locus_tag="ACHOQQ_RS02320"
                     /old_locus_tag="pgaptmp_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGU4468769.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9645 a        11072 c        10682 g         9067 t
ORIGIN      
        1 ataattcaat acaaatcatc aggataggtt ttgcaacgcg tgcattttgt cccctttttc
       61 ctcgtggatt agatgcaaaa atttattctg aaatatgtca accgatgaaa agcgtcggta
      121 gttaatcaga aattaatatc gcttgtttta accacagcag cacaattagc taattttacg
      181 gatgcagaac tcatgctggc gggacgtttt tattgcgtca gggttgacat ccgtttttgt
      241 atccagtaac tctaaaagca tatcgcattc atctggagct gatttaatga ctcacatcgt
      301 tcgctttatc ggtctactac tactaaacgc atcttctttg cgcggtagac gagtgagcgg
      361 catccagcat taagccagca cgcagtcaaa caaaaaaccc gcgccattgc gcgggttttt
      421 ttatgcccga agcgaggcgc tctaaaagag acaaggaccc aaaccatgag ccagcaagtc
      481 attattttcg ataccacatt gcgcgacggt gaacaggcgt tacaggcaag cttgagtgtg
      541 aaagaaaaac tgcaaattgc gctggccctt gagcgtatgg gtgttgacgt gatggaagtc
      601 gggttccccg tctcttcgcc gggtgatttt gaatcagtgc aaaccatcgc tcgccaggtt
      661 aaaaacagcc gcgtatgcgc gttagctcgc tgcgtggaga aagatatcga cgtggcggcc
      721 gaatccctga aagtcgccga agccttccgt attcatacct ttattgccac ttcgccaatg
      781 cacatcgcca ccaagctgcg cagcacgctg gacgaggtga tcgaacgcgc tatctatatg
      841 gtgaaacgcg cccgtaatta caccgatgat gttgaatttt cttgcgaaga tgccgggcgt
      901 acacccattg ccgatctggc gcgagtggtc gaagcggcga ttaatgccgg tgccaccacc
      961 atcaacattc cggacaccgt gggctacacc atgccgtttg agttcgccgg aatcatcagc
     1021 ggcctgtatg aacgcgtgcc taacatcgac aaagccatta tttccgtaca tacccacgac
     1081 gatttgggcc tggcggtcgg caactcactg gcggcggtac atgccggtgc acgccaggtg
     1141 gaaggtgcaa tgaacgggat cggcgagcgt gccggaaact gttcactgga agaagtcatc
     1201 atggcgatca aagttcgtaa ggatattctc aacgtccaca ccgccatcaa tcaccaggag
     1261 atatggcgca ccagccagtt agttagccag atttgtaata tgccgatccc ggcaaacaaa
     1321 gccattgttg gcagcggcgc attcgcacac tcctccggta tccaccagga tggtgtgctg
     1381 aaaaaccgcg aaaactacga aatcatgaca ccagaatcta ttggtctgaa ccaaatccag
     1441 ctgaatctga cctctcgttc ggggcgtgcg gcggtgaaac atcgcatgga tgagatgggg
     1501 tataaagaaa gtgaatataa tttagacaat ttgtacgacg ccttcctgaa gctggcggac
     1561 aaaaaaggtc aggtgtttga ttacgatctg gaggcgctgg ccttcatcgg taagcagcaa
     1621 gaagagccgg agcatttccg tctggattac ttcagcgtgc agtctggctc taacgatatc
     1681 gccaccgccg ccgtcaaact ggcctgcggc gaagaagtca aagcagaagc cgccaacggt
     1741 aacggtccgg tcgatgccgt ctatcaggcg attaaccgca tcactgacta taacgtcgaa
     1801 ctggtgaaat acagcctgac cgctaaaggt cacggtaaag atgcgctggg tcaggtggat
     1861 attgtcgcca actacaacgg tcgccgcttc cacggcgtcg gcctggccac cgatattgtc
     1921 gagtcctccg ccaaagccat ggtgcatgta ctgaacaata tctggcgcgc cgcagaagtc
     1981 gaaaaagagt tgcaacgcaa agctcaacac aacgaaaaca acaaggaaac cgtgtgatgt
     2041 cgaagaatta ccatattgcc gtattgccgg gggacggtat tggtccggaa gtgatgaccc
     2101 aggcgctgaa agtgctggat gccgtgcgca accgctttgc gatgcgcatc accaccagcc
     2161 attattatgt aggcggcgca gccattgata accacgggca gccgctgccg cctgcgacgg
     2221 ttgaaggttg tgagcaagcc gatgccgtgc tgtttggctc ggtaggcggt ccgaagtggg
     2281 aacatttacc accagaccag caaccagaac gcggcgcgct gctgcctctg cgtaagcact
     2341 tcaaattatt cagcaacctg cgcccggcaa aactgtatca ggggctggaa gcattctgtc
     2401 cgctgcgtgc agacattgcc gcaaacggct tcgacatcct gtgtgtgcgt gaactgaccg
     2461 gcggcatcta tttcggtcag ccaaaaggcc gcgaaggtag cggacaatat gaaaaagcgt
     2521 ttgataccga ggtgtatcac cgttttgaga ttgaacgtat cgcccgcatc gcatttgaat
     2581 ctgctcgcaa gcgtcgccac aaagtcacct caatcgacaa agctaacgtg ctgcaatcct
     2641 ctattttatg gcgtgaaatc gtcaacgaga tcgccacgga atacccggat gtcgaactgg
     2701 cgcatatgta catcgacaac gccaccatgc agctgattaa agatccatca cagtttgacg
     2761 ttctgctgtg ctccaacctg tttggcgaca ttctgtctga cgagtgcgca atgatcactg
     2821 gctcgatggg gatgttgcct tccgccagcc tgaacgagca aggttttgga ctgtatgaac
     2881 cggcgggcgg ctcggcacca gatatcgcag gcaaaaacat cgccaacccg attgcacaaa
     2941 tcctgtcgct ggcactgctg ctgcgttaca gcctggatgc cgatgatgcg gcttccacca
     3001 ttgaacgcgc cattaaccgc gcattagaag aaggcattcg caccggggat ttagcccgtg
     3061 gcgctgccgc cgttagtacc gatgaaatgg gcgatatcat tgcccgctat gtggcagaag
     3121 gggtgtaatc atggctaaga cgttatacga aaaattgttt gacgctcacg tagtgtacga
     3181 agccgaaaac gaaaccccgc tgttatatat cgaccgccac ctggtgcatg aagtgacctc
     3241 accgcaggcg ttcgatggtc tgcgcgccca cggtcgcccg gtacgtcagc cggggaaaac
     3301 cttcgccacc atggatcaca acgtttcaac tcagaccaaa gacattaatg cctgcggtga
     3361 aatggcgcgc atccagatgc aggagctgat caaaaactgc aaagaatttg gcgtcgagct
     3421 gtatgacctg aatcacccgt atcaggggat cgtccacgtg atggggccgg aacagggcgt
     3481 gaccttgccg gggatgacca ttgtctgcgg cgactcgcac accgccaccc acggcgcgtt
     3541 tggcgcactg gcctttggta tcggcacttc cgaagttgaa cacgtactgg caacgcaaac
     3601 cctgaaacag ggccgtgcga agaccatgaa aattgaagtc cagggcaaag ccgcgccggg
     3661 cattaccgca aaagatatcg tgttggcaat tatcggtaaa actggcagcg caggcggcac
     3721 cgggcatgtg gtggagtttt gcggcgaagc aatccgtgat ttaagcatgg aaggtcggat
     3781 gaccctgtgc aatatggcaa tcgaaatggg cgcgaaagcc ggtctggttg caccggacga
     3841 aaccaccttt aactatgtca aaggtcgtct gcatgcgccg aaaggcaaag atttcgacga
     3901 cgccgttgcc tactggaaaa ccctgcaaac cgacgaaggc gcaactttcg ataccgttgt
     3961 cactctgcaa gcagaagaga tttcgccgca ggtcacctgg ggcaccaatc caggccaggt
     4021 gatttccgtg aacgacaata ttcccgatcc agcttcgttt gccgatccgg ttgaacgcgc
     4081 gtcggcagaa aaagcgctgg cctatatggg gctgaaaccg ggtattccgc tgaccgaagt
     4141 ggctatcgac aaagtgttta tcggttcctg taccaactcg cgcattgaag atttacgcgc
     4201 ggcagcggag atcgccaaag gccggaaagt cgcgccaggc gtgcaggcac tggtggttcc
     4261 cggctctggc ccggtaaaag cccaggcgga agcggaaggt ctggacaaaa tctttattga
     4321 agccggtttt gaatggcgct tgcctggctg ctcaatgtgt ctggcgatga acaacgaccg
     4381 tctgaatccg ggcgaacgtt gtgcctccac cagcaaccgt aactttgaag gccgtcaggg
     4441 gcgcggcggg cgcacgcatc tggtcagccc ggcaatggct gccgccgcgg ctgtgaccgg
     4501 ccatttcgcc gacattcgca acattaaata aggagcacac catggcagag aaatttatca
     4561 aacacacagg cctggtggtt ccgctggatg ccgccaatgt cgataccgat gcaatcatcc
     4621 cgaaacagtt tttgcagaaa gtgacccgta cgggttttgg cgcgcatctg tttaacgact
     4681 ggcgttttct ggatgaaaaa ggccaacagc caaacccgga cttcgtgctg aacttcccgc
     4741 agtatcaggg ggcttccatt ttgctggcgc gggaaaactt cggctgtggc tcctcgcgtg
     4801 agcacgcgcc ctgggcattg accgactacg gttttaaggt ggtgattgcg ccgagttttg
     4861 ctgacatctt ctatggcaat agctttaaca accagctgct gccggtgaaa ttaagcgacg
     4921 cagaagtgga tgagctgttt gcgctggtga aagccaatcc ggggattcat tttgatgtag
     4981 acctggaagc gcaagaggtg aaagcgggtg agaaaaccta tcgctttacc attgatgcct
     5041 tccgccgcca ctgcatgatg aacggtctgg acagcattgg gcttaccttg cagcacgacg
     5101 acgccattgc ggcttatgaa gcgaagcaac ccgcgtttat gcgttagttt gtacaccgtc
     5161 cggtactcaa tcgccaaaaa gaaaaaagcc agcagataat cactgctggc gtggcgatta
     5221 acaccaatac tcagtcacac atgatgtccg caaatcagta gtcagaatga ctcccctccc
     5281 actgcatcag ttcttcaagc atggttaatc gttgtgggac gcttaaccaa cgcaaccagc
     5341 acaacttcgc tgtcgcggta aaatagtgct gataaaactg acgcatgggg cacctccttg
     5401 cttcatcgtt aggaattatt gacttaatat agggaaaata aaattgctgt cttttgcgca
     5461 ggagttcccc ttttatgcca tctgctcgtc tgcaacaaca gttcatccgc ctgtggcaat
     5521 gctgcgaggg taaatcgcag gacacaacgc tcaacgaact ggcagcgtta ttgagctgct
     5581 cgcgtcgtca tatgcgcacc ctgctcaaca ccatgcagga tcgcggctgg ctgacgtggg
     5641 aagcggaagt cgggcgtggt aaacgctcgc gtctgacatt cctctatacc gggctggcgc
     5701 ttcagcaaca gcgggcggaa gacctgctgg agcaggaccg tatcgaccaa ctagtgcagt
     5761 tagttggcga caaagcgact gtgaggcaaa tgctggtttc tcatctgggc cgtagcttcc
     5821 gccaggggcg gcacatcctg cgcgtgctct actatcgtcc gttgcgtaat ctgctacctg
     5881 gcagcgcatt gcgccgttcc gaaacccata tcgcccggca aatcttcagt tcgctaacgc
     5941 gcataaatga ggaaaatggg gaactggaag cagacatcgc ccaccactgg cagcaaatat
     6001 caccgcttca ctggcgtttc tttttgcgtc caggagtcca ttttcatcat ggtcgtgaac
     6061 tggaaatgga cgacgtgatc gcctctttaa aacgaatcaa tacgctgccg ctctattcgc
     6121 atattgctga cattgtgtcg ccgacgccct ggacgctgga tatccacctc acgcagccgg
     6181 atcgctggtt gccgttactg ctgggacaag ttccggcgat gatcctgccg cgcgaatggg
     6241 aaaccctcag taactttgcc agccatccca tcggcaccgg tccgtatgcg gtgattcgca
     6301 acagcaccaa tcaactgaaa attcaggcat tcgatgactt cttcggttac cgggcattaa
     6361 tcgatgaagt taatgtctgg gttctgccgg aaattgccga cgagccagcc ggagggctga
     6421 tgctgaaagg cccacagggc gaggaaaaag agattgaaag ccgactggag gaaggttgtt
     6481 actatttact gttcgacagc cgaacccatc gcggggcgaa tcaacaagtc agagactgga
     6541 taagctatgt gctttcgccg accaatctgg tctatttcgc cgaggaacag taccagcaac
     6601 tgtggtttcc ggcctatggg ctgctccccc gctggcacca cgcccgccca acacattgcg
     6661 aaaaacctgc cgggctggaa catctcaccc tgacgtttta tcaggatcat attgaacatc
     6721 gggtgattgc caggatcatg cagcagattc tggcgagtca tcaggtcacg atggaaatca
     6781 aagagatcag ctacgatcaa tggcacaaag gtgagatcga aagcgacata tggcttaaca
     6841 gcgccaactt tacgctgccg ctggattttt cgctgttcgc gcacttgtgc gaagtacccc
     6901 tgctgcaaca ctgtattccg ctcgactggc aagccgacgc agcccgctgg cgcaatggcg
     6961 agataaatct ggcgaactgg tgccagcaac tggtcgccag taaagcaatg gtgccactta
     7021 tccaccactg gctgatcatt caggggcaac gcagtatgcg cggcctgcgc atgaataccc
     7081 tcggctggtt cgattttaaa tcagcgtggt ttgcgccgcc ggatccagag tagcttgcgc
     7141 cttttggcgg tcatgagcgc attttttata ttcagacgga atgataaaat acattacttt
     7201 tatattttct ctgttttatg catcgtctga ttagcgtcgc aaataagatt aaagaagggg
     7261 cattccagcc cctctttcat attctgagtt aatcaataac ggcgcagtct agccttgttc
     7321 tggccggaaa gatcatcttt ttcaggttaa cctcgcgcag gtcagtcata ccaacggcta
     7381 ttgttcgttt cagcattgct caggtcaacc tcgagacagt caataccaca aatgacaacc
     7441 ccgttaaagt aagcattact cagatcaatg cacccggtcc tggtgttctc tttcagctgc
     7501 aacttcagag taaatatcgc ctcatcgcca ataaaaaaac tgacgcataa cgtatctttt
     7561 ggcgagagtg aggccttcgc ggccatattt tccggatctc ttgcgtgttc tttgagttca
     7621 ttaaatttta tcagccagtg agttaactga tcacgcgcct tcccgtcgac agccccggta
     7681 aagtattttg agtagtcatc actattctgg aaataaaatg catccttcag aatttgcctt
     7741 gccgcagtct gggaatgttt atctgaccgg aagagattgc gtaacgtctc ccatagctct
     7801 tcaaagatat tcatattctc ggtctttttg accaagttag ccatcctgat aatctgagaa
     7861 ttagtaaggc ctggatcttt agtggtatta ctagcaggga tactaatctg ttctgggttt
     7921 cttactttca tgctgatagc tccactctga gtaaaaaact cagaataagc gtcctgaata
     7981 agaatactgt caaaaatcgc tcattcacgc aataaacggt tacgttacct attttgactg
     8041 tagcgaaggg attctttcgg atcaacgctc tccagctatg caaatacgct cttgatagct
     8101 gaacgaatat tggctttttt aatacgcctt ctctatggat tgctggttga taacaaaatc
     8161 actacactaa cgccgttctc aacggggtgc cacgcggacg cgtgcgctga gaaaataccc
     8221 gtcgaacctg atccggataa cgccggcgaa gggatttgag gctccttctc aagtcctttg
     8281 ccactctttt ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac
     8341 agcgcccgtt ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga
     8401 ctgggggcca ggtccggtgg ttaaaaaagc ctttgaagcc gactgtaatt gcgaactgaa
     8461 actggtggcg ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa
     8521 cagtaaagcc gatgtggtgc tggggctgga taacaacctg ttagacgccg ccagtaaaac
     8581 cggactgttt gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa
     8641 taatgacact ttcgtaccgt ttgattatgg ctacttcgcc ttcgtttatg acaagaacaa
     8701 actgaaaaac ccgccacaaa gcctgaaaga actggttgag agcgatcaaa actggcgggt
     8761 gatttatcag gatccgcgca ccagtacacc ggggctgggt ctgttgctat ggatgcaaaa
     8821 agtctatggc gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ccgtcaccgt
     8881 caccaaaggc tggagcgaag cctacggcct gtttttaaaa ggtgaaagcg atctggtact
     8941 gagttacacc acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc
     9001 cgcgaacttc agcgaaggtc actatctgca agtggaagtc gccgctcgca ccgctgccag
     9061 caagcagccg gagctggcgc aaaaattcct ccagtttatg gtttctccgg ctttccagaa
     9121 tgcgatccca accggcaact ggatgtatcc ggtggcaaac gtcacgctgc ctgccggttt
     9181 tgaaaaattg accaaacccg caaccacgct ggagttcacg ccagccgaag tggcggcaca
     9241 acgtcaggca tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta
     9301 attccaggtg taagcgccgc cacgctggtg atagcggttg cgctggcggc gtttctcgcc
     9361 ctgtggtcga acgcgccgca gggtgactgg tcggcagtct ggcaggacag ctacctgtgg
     9421 catgtggtgc gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc
     9481 gcgatattcc tcgcccgcgc gctctatcgc aggcgctttc cgggtcggct ggcgctgttg
     9541 cgtctgtgcg caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc
     9601 gtctatggtc gccagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc
     9661 ttttcgccct acggcctgca aggtattttg ctggcgcacg tattttttaa tctaccgatg
     9721 gcgagccgct tattactcca ggcactggaa aacattcccg gcgaacaacg tcagcttgcc
     9781 gcccagcttg ggatgcgcgg ctggcatttt ttccgcttcg tcgaatggcc gtggttacgg
     9841 agacaaatcc cgccggttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc
     9901 gtactgtcgc tgggcggcgg tccgcaggcg accactatcg agctggcaat ctatcaggcg
     9961 ctgagttacg actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatggtgtgt
    10021 tgccttgggc tggtgctgtt gagtcagcga ctgagtaagg ccattgcgcc cggcaccacg
    10081 ctgctgcaag gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg
    10141 ttaattgtgc tggcgctgct gctgttgctt ccaccgttac tggcggtgat cgtcgatggg
    10201 gtaaatcgcc agttgccgga agtgctggca caaccggtac tgtggcaggc gctgtggacc
    10261 tcgttgcgta ttgcgctggc ggcaggcgta ttgtgcgtag tgctgaccat gatgctgcta
    10321 tggagcagtc gcgaactgcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg
    10381 agtggcatgt tgatcctcgc catgccgggg attgtgctgg caacgggctt ctttttactg
    10441 ctcaacaaca ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg
    10501 ttaatggcga tcccctacgc cctgaaagtg ctggaaaacc cgatgcgcga tatcaccgcc
    10561 cgctacagca tgttatgtca gtcgctgggg attgaaggct ggtcacgctt aaaagtggtg
    10621 gagctgcgcg ccctgaaacg tccactggcg caggcgctgg cctttgcctg cgtgctgtcg
    10681 attggtgatt ttggcgtggt agcgttgttc ggtaacgatg atttccgcac cctgccgttt
    10741 tatctctacc agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt
    10801 ctgctgctac tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt
    10861 aaaactgact gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacagt
    10921 agaacgcggc gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct
    10981 gaatttgatt gccggttttc tgacgccagc cagcggttcg ctgactatcg atggcgttga
    11041 tcacaccacg acgccgccgt cacgccgtcc ggtgtcgatg ctgtttcagg agaacaacct
    11101 gttcagccac ctgacggtcg cgcagaacat cgggctgggg ctaaatccgg gattgaaact
    11161 gaacgcggca cagcaggaga aaatgcacgc gattgcccgt cagatgggga ttgataattt
    11221 aatggcgcgg ttacctggcg agctttccgg cggtcagcga cagcgagtgg cgttagcgcg
    11281 ttgtctggta cgcgaacagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc
    11341 ggcgttacgt caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac
    11401 gttattgatg gtgtcgcaca gtgtggaaga tgcggcaagg attgcaacac gctcgatagt
    11461 ggtcgccgac gggcgcatcg cctggcaggg taagaccgaa gaattgttga gcggtaaggc
    11521 gagtgcttcg gcgctgttgg gaattacagg ttaatatgca ctgtcggatg cggcgtgaac
    11581 gccttatccg acctacgggt gcgttttgtc cgcatcaagc gccccacaca agactatgcc
    11641 ctaaactcca accactttac gcaaaatatc gatatacacc ggcattaacg gatggcgaat
    11701 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    11761 accgcgggac aaataatgac tcaaacggtc agtcgcttta ccactgcgcc ataaccgcca
    11821 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    11881 accgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc ctggcaggaa
    11941 gtaaaacggc ggccacagca ggcagccgat aatattcggc gtaataaatt tagccaccgg
    12001 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaaacgacc
    12061 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    12121 tttgttcttc ttcagaaatg accagcgatg caatggcttt ttaaaacgcc agcccagcca
    12181 gaaggagatc cagtcgccca gcagacaacc gacgatccct gccagccagg cgtgccagaa
    12241 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    12301 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    12361 atacacggtg gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    12421 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    12481 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    12541 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat
    12601 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    12661 gcttcgcctg gctgatgcgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    12721 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    12781 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
    12841 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    12901 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    12961 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg
    13021 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    13081 aaacccactg gtgataccat tcgcgagcct ccggatgacg gccgtagtga tgaatctctc
    13141 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    13201 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    13261 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    13321 cgttaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    13381 tcccgccatt cagagaagaa accaattgtc catattgcat cagatattgc cgtcactgcg
    13441 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    13501 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    13561 gaaaagtcca cattgataat ttgcacggcg tcacactttg ctatgccata gcatttttat
    13621 ccataagatt agcggatcct acctgacgct ttttttcgca actctctact gtttctccat
    13681 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    13741 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    13801 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    13861 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    13921 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    13981 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccagagtt tgccgaaaac
    14041 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    14101 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctacattgg cggtatttat
    14161 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    14221 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    14281 acgcgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataagtc gctatggcac
    14341 gaaagctggg gcggcctgcc gccagccagt ttctttgacg aactggaccc gatcctcaat
    14401 cgccatttgc cttccccgct gttcactgat acctggactg ccgatattcc ggtgggcacc
    14461 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    14521 gcgtttgact gccatatggg cgcagttggt gcaggcgcac agcctaacgc actggtaaaa
    14581 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    14641 gcagtgaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    14701 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt tcggtcgcgt actcggctgg
    14761 ccgctggaac agcttgccgc ccagcatccg gaactaaaag agcaaattaa cgccagccag
    14821 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacatg
    14881 ccggtggtgc tcgactggtt taacggccgc cgcacaccga acgctaacca acgcctgaaa
    14941 ggggtgatta ccgaccttaa cctcgctacc gatgctccgc tgctgttcgg cggtttgatt
    15001 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    15061 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    15121 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    15181 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    15241 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgt gcagcgagca ggcacaacgc
    15301 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    15361 ccaacttccg ccccggcaca ggctgcccag gccgttccga ctctataagg acacgataat
    15421 gacgattttt gataattatg aagtgtggtt tgtaattggc agccagcatc tgtatggccc
    15481 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc taaatacgga
    15541 agcgaaactg ccctgcaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    15601 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tatggctgca
    15661 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    15721 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    15781 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    15841 tcagcaacat gccgtggtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    15901 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgttt
    15961 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacaaatcaa
    16021 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    16081 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    16141 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    16201 ggggatgaaa cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    16261 tttgcacggt ctgaaacagc ttccaggtct ggccgtacag cgtctgatgc agcagggtta
    16321 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    16381 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    16441 aggcaatgac ctggtgctcg gctcccacat gctggaagtc tgcccgtcga tcgccgcaga
    16501 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    16561 cctgatcttc aatacccaaa ccggcccagc gattgtcgcc agcctgattg atctcggcga
    16621 tcgttaccgt ctactggtta actgcatcga cacggtgaaa acaccgcact ccctgccgaa
    16681 actgccggtg gcgaatgcgc tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    16741 gtggatcctc gcaggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    16801 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    16861 cctgccagcg tttaaagacg cgctgcgctg gaacgaagtg tattacggat ttcgtcgcta
    16921 agtagccgca tcaggtgtgt aacgcctgat gcgacgctga cgcgtcttat caggcctaca
    16981 cgctgtgatt ttgtaggccg gataagcaaa gcgcatccgg cattgttttc cccctcgccc
    17041 ctttggggag agggccgggg tgaggggaaa cgacctctca ggagtcaaca tgttagaaga
    17101 tctcaaacgc caggtattag aagccaattt ggcgctgcca aaacataacc tggtcacgct
    17161 cacctggggc aacgtcagcg ccgttgatcg cgagcgcggc gtctttgtga tcaaaccttc
    17221 cggcgtcgat tacagcatca tgaccgccga cgatatggtc gtggtcagca tcgaaaccgg
    17281 tgaagtggtt gaaggtacga aaaagccctc ctccgacacg ccaactcacc ggctgctcta
    17341 tcaggcattc ccgtccattg gcggcattgt gcacacacac tcgcgccacg ccaccatctg
    17401 ggcgcaggcg ggtcagtcga ttccagcaac tggtaccacc cacgccgact atttctacgg
    17461 caccattccc tgcacccgca aaatgaccga cgcagaaatc aacggcgaat atgagtggga
    17521 aaccggtaac gtcatcgtag aaaccttcga aaaacagggt atcgatgcag cgcaaatgcc
    17581 cggcgttctg gtccattctc acggcccatt tgcatggggc aaaaatgccg aagatgcggt
    17641 gcataacgcc atcgtgctgg aagaggtcgc ttatatgggg atattctgcc gtcagttagc
    17701 gccgcagtta ccggatatgc agcaaacgct gctggataaa cactatctgc gtaagcatgg
    17761 cgcgaaggca tattacgggc agtaatgact gtataaaacc acagccaatc aaatgaaacc
    17821 aggctataat caagcctggt tttttgatgg attttcagcg tggcgcaggc aggttttatc
    17881 ttaacccgac actggcggga taccccgcaa ggaacagagg tctccttctg gctggcgacg
    17941 gacaacgggc cgttgcaggt tttccttgcg ccgcaagagt ccgtggcgtt tattcccgcc
    18001 gatcaggttc cccgcgctca gcatattttg cggggtgaac aaggctttcg cctgacgccg
    18061 ctggcgttaa aggattttca ccgccagccg gtgtatggcc tttactgtcg cgcccatcgc
    18121 cagttgatga attacgaaaa gcgcctgcgt gaaggtggcg ttaccgtcta cgaggccgat
    18181 gtgcgcccgc cagaacgcta tctgatggag cggtttatca cctcgccggt gtgggtcgag
    18241 ggtgatatgc acaatggcgc tatcgttaat gcccgtctga aaccgcatcc cgactatcgc
    18301 ccgccgctca agtgggtgtc tattgatatt gaaaccaccc gccacggtga gctgtattgc
    18361 atcggcctgg agggctgcgg gcagcgcatc gtttatatgc tggggccgga gaatggcaac
    18421 gcttccgcgc tcgatttcga actggaatac gtcgccagtc gcccgcagct actggaaaaa
    18481 cttaacgcct ggtttgccaa ctacgatcct gatgtgatca tcggttggaa cgtggtgcaa
    18541 ttcgatctgc gaatgctgca aaaacatgcc gagcgttatc gtattccgct gcggctgggg
    18601 cgcgataaca gcgagctgga gtggcgcgag cacggcttta aaaacggcgt cttttttgcc
    18661 caggctaaag ggcggctaat tatcgacggt atcgaggcgc tgaaatccgc gttctggaat
    18721 ttctcttcat tctcgctgga aactgtcgct caggagctat taggcgaagg aaaatctatc
    18781 gataacccgt gggatcgaat ggacgaaatt gaccgccgtt tcgccgaaga taaacctgcg
    18841 ctggcgactt ataacctgaa agattgcgag ctggtgacgc agattttcca taaaactgaa
    18901 atcatgccgt ttttgctcga acgagcaacg gtgaacggcc tgccggtaga ccgccacggg
    18961 ggatcggtgg cggcgtttgg tcatctctat tttccccgga tgcatcgcgc tggttatgtc
    19021 gcgcctaatc tcggcgaagt gccgccgcac gccagccctg gcggctacgt gatggattcg
    19081 cgacctgggc tttatgattc ggtgctggtg ctggactaca aaagcctgta cccgtcgatc
    19141 atccgcacct ttctgattga tcccgtcggg ctggtggaag gcatggcgca gcctgatcca
    19201 gagcacagca ccgaaggctt tctcgatgcc tggttctcgc gagaaaaaca ttgcctgccg
    19261 gagattgtga ctaacatctg gcacgggcgc gatgaagcca aacgtcaggg taataagccg
    19321 ctttcgcagg cgctgaagat cattatgaat gccttttatg gcgtgctcgg caccaccgcc
    19381 tgccgcttct tcgatccgcg gctggcatcg tcgatcacca tgcgtggtca tcagatcatg
    19441 cggcaaacca aagcgttgat tgaagcgcag ggctacgacg tgatctacgg cgatactgac
    19501 tcaacgtttg tctggctgaa aggcgcacat tcggaagaag aagcggcgaa aatcggtcgt
    19561 gcactggtgc agcacgttaa cgcctggtgg gcggaaacgc tgcaaaaaca acagctgacc
    19621 agcgcactgg aactggagta tgaaacccat ttctgccgtt ttctgatgcc aaccattcgc
    19681 ggagccgata ccggcagtaa aaagcgttat gccgggctga ttcaggaggg cgacaaacag
    19741 cggatggtgt ttaaagggct ggaaaccgtg cgtacggact ggacgccact ggcccagcag
    19801 tttcagcagg agctttacct gcgtatcttc cgcaacgagc catatcagga gtacgtccgc
    19861 gaaaccatcg acaaactgat ggcgggtgaa ctggatgcgc ggctggttta ccgtaaacgc
    19921 cttcgccgtc cgctgagcga atatcagcgt aatgttccgc ctcatgtacg cgccgctcgc
    19981 cttgccgatg aagaaaacca aaagcgcggt cgccccttgc aataccagaa ccgcggcacc
    20041 attaagtacg tatggaccac caacggcccg gagccgctgg actaccaacg ttcaccactg
    20101 gattacgaac actatctgac ccgccagcta caacccgtgg cggagggaat actccctttt
    20161 attgaggata attttgctac acttatgacc gggcaacttg ggctattttg agcaaaaaaa
    20221 gagttcgcca gataccattt tgatgcgtga cgaatgcttt gccatccagt accatagcgc
    20281 cctttccatt cctggacctg aataacacca ctacctcata agcacggtag cgggtggtta
    20341 ttgcctgcaa ttaaagatat agagccgaac acatatgcct tttacacttg gtcaacgctg
    20401 gatcagcgat acagaaagcg aattgggact tggaaccgtt gtcgcggtgg atgcgcgaac
    20461 tgtcacttta cttttcccat ctactggtga aaaccgtctg tacgcacgca gtgattcccc
    20521 cgtgacccgc gtgatgttca accctggtga taccattacc agccatgacg gctggcagat
    20581 gcaagtcgaa gaagtaaaag aagaaaatgg cttgctgacc tatatcggta ctcgcctgga
    20641 tactgaagag tccggcgtag ccctgcgtga agttttcctt gatagcaaac tggtgttcag
    20701 caaaccgcag gaccgcctgt ttgccgggca gattgaccgt atggaccgct ttgcgctgcg
    20761 ttatcgcgcg cgtaagtatt ccagcgaaca gttccgtatg ccgtacagcg gcttgcgcgg
    20821 tcagcgtacc agcctgatcc cgcaccagct caacatcgct catgatgttg gtcgccgcca
    20881 cgcgccgcgc gtcctgctgg ctgacgaagt gggtttaggg aaaaccattg aagccgggat
    20941 gatcctgcat cagcaactgc tctctggcgc tgctgaacgt gtgctgatta ttgtcccgga
    21001 aaccttacag catcagtggc tggtagaaat gctgcgccgt ttcaacctgc gttttgcgct
    21061 gtttgatgat gagcgttatg ccgaagctca gcacgatgct tacaacccgt tcgataccga
    21121 acaactggtg atttgctcgc tggattttgc ccgtcgtagc aaacagcggc tggaacatct
    21181 ctgtgaagcc gaatgggacc tgctggtggt cgatgaagcc catcacctgg tgtggagcga
    21241 agatgcgcca agccgtgaat atcaggccat tgaacaactg gcagagcacg tgccgggcgt
    21301 tctgctgctg accgcgaccc cggaacagct ggggatggaa agccacttcg cccgtctgcg
    21361 tctgctggac ccgaaccgtt tccacgattt tgcgcagttc gttgaagagc agaaaaatta
    21421 tcgtccggtt gcggacgccg ttgccatgct gctggcaggt aataaactga gcaatgacga
    21481 actgaacatg ctcggcgaga tgatcggcga gcaggatatc gagccgctgt tgcaagcagc
    21541 aaacagcgac agcgaagatg cccagagcgc ccgtcaggaa ctggtttcga tgctgatgga
    21601 tcgccacggc accagccgcg tgctgttccg taacacccgt aacggtgtga aaggcttccc
    21661 gaaacgcgag ttgcacacca ttaagctgcc gctaccgacg cagtatcaga cggctattaa
    21721 agtctccggc attatgggcg cacgtaaaag tgcggaagat cgtgctcgag atatgctcta
    21781 cccggagcgt atttatcagg aatttgaagg tgataacgcc acctggtgga acttcgatcc
    21841 gcgcgttgag tggctgatgg gctacctgac cagccatcgc tctcagaaag tgctggtgat
    21901 ctgcgctaaa gctgccactg cgctgcaact ggagcaagta ctgcgcgaac gtgaaggtat
    21961 ccgcgctgct gtgttccacg aaggcatgtc gattattgag cgtgaccgcg ctgccgcctg
    22021 gtttgctgaa gaagacaccg gcgcacaggt actgctgtgc tcggaaatcg gttctgaagg
    22081 acgtaacttc cagttcgcca gccacatggt gatgtttgac ctgccattca acccggatct
    22141 gctggagcag cgtattggtc gtctggatcg tatcggtcag gcgcacgata ttcagatcca
    22201 tgtgccttat ctggaaaaaa ctgctcagtc ggtgctggtg cgctggtatc acgaaggtct
    22261 ggatgcattc gagcacacct gcccgaccgg acgcactatt tacgatagcg tctacaacga
    22321 tctgattaac tatctggctt caccggatca aaccgaaggc tttgacgatc tgatcaaaaa
    22381 ctgccgcgag caacatgaag cgctgaaagc acaactggaa caggggcgtg accgcctgct
    22441 ggaaatccac tccaacggtg gcgaaaaagc ccaggcactg gcagaaagca ttgaagagca
    22501 ggatgacgat accaacctga tcgccttcgc catgaacctg ttcgatatta tcggtatcaa
    22561 tcaggacgat cgcggcgaca acatgatcgt gctgaccccg tccgatcata tgctggtgcc
    22621 ggacttccct ggcctgtcgg aagatggcat caccatcacc tttgatcgtg aagtggcgct
    22681 ggcgcgtgaa gatgcacagt ttattacctg ggagcatccg ctgatccgca acggtctgga
    22741 tctgatcctt tctggcgata ctggtagcag cacgatttca gtgttgaaaa acaaagcgtt
    22801 accggtaggt acgctgttgg tggaactgat ttacgtggtc gaagcccagg cgccgaagca
    22861 gttgcagctc aaccgcttcc tgccaccgac gccggtacgt atgctgctgg ataaaaatgg
    22921 caacaacctg gcggcccagg tggagtttga aacctttaac cgccagctta acgcggtcaa
    22981 ccgtcacacc gacagcaaac tggttaatgc cgtgcagcag gatgttcacg ctatccttca
    23041 gctgggtgaa gcgcagatcg agaaatcggc ccgtgcattg attgataccg cacgtaacga
    23101 agccgacgaa aaactgtctg ccgagctgtc tcgtctggaa gcgctgcgcg cagtgaaccc
    23161 gaacattcgt gacgacgaac tgaccgccat tgaaagcaac cgtcagcagg taatggaaag
    23221 cctggatcag gcaggctggc gtctggatgc cctgcgtttg atcgttgtaa cgcatcagta
    23281 acggagccga aaatggggat ggaaaactac aatccaccgc aggaaccctg gttggttatc
    23341 ctgtatcagg atgaccacat tatggtggtc aacaagccga gcggtttgtt gtctgtgccg
    23401 ggtcgtctgg aagagcacaa agacagcgtg atgacgcgca ttcagcgtga ttatccgcag
    23461 gcagaatcgg tgcatcgtct ggatatggct accagcggcg tgattgtagt ggcgctgacc
    23521 aaagccgcgg agcgggagtt aaaacgccag ttccgcgagc gcgagccgaa aaagcagtat
    23581 gtggcccgcg tctgggggca tccatcccct gcggaaggtc tggtagatct gccgctgatt
    23641 tgcgactggc caaaccgccc gaaacagaaa gtctgttacg aaacaggtaa acctgcgcag
    23701 acggaatatg aagtggtgga gtatgcggcg gataacacgg caagagtggt gttaaaaccg
    23761 attaccgggc gttcgcatca attgcgtgtg catatgctgg cgctgggtca tccgattctc
    23821 ggcgatcgtt tttatgcatc accggaagcg agagcgatgg caccgcgttt gttgctacat
    23881 gcagagatgt tgacgattac ccatccggcg tatggcaata gtatgacgtt taaagcgcca
    23941 gcggattttt aatagtcaca ctggcgaagt tcggttggcc ggagaaggcg ttcacgccgc
    24001 atccggcaat tttgcgccga ttgcctgatg cgacgcgggc cgcgtcttat caggcctaca
    24061 gtttgaaact tatttaaacc ctttctgctg cttaatcaat tcataggcct gctgaatttc
    24121 ctgcgctttt tgcttcgcca tctccatcat ctcaggcggc aaacctttcg ccaccaactt
    24181 atcgggatgg tgttcactca tcagcttacg gtaggcacgt ttgatggtgg tcgcatcatc
    24241 cgtcggcttc acgcccagca cattacaggc atcttccagc gtcgggccac gctgggtttg
    24301 ttgccagtta tcaccgccag attgctgctg ataaccgccg ccaaactgtg caccgccctg
    24361 catcatgcgc aaaaactggt caaactgggc gcgggagatc cctaattctt cagcaatgac
    24421 atacagcacc gcccgttcat tcgggtgcag tgaaccatca gcaaacgccg cctgaatctg
    24481 gatctccaga aacatacgaa ttaagtcaaa acgaccaaag cagacactgc gaaactggcg
    24541 catcttttcg cgcagcgggt aattgtctga ttttcccacc cggaacgcgt tttgcgccgc
    24601 agtacgggaa gcgccatgaa ggttcattcg gtccatcaac tggctggcga tatgaatatc
    24661 agcctccgtg acgcgacctt tggatttggt taaatgcccc atcacttcaa aagtggtggc
    24721 aaaaaacagc gcctgacgct cacgctggtt ggcaaaccac gccattttac ggctacgggc
    24781 tttatcaaac atatggccaa ttaacaggcc taacactacg ccccaaaagc cgccgcccat
    24841 cagtaaggcc acggccacgc caatgatttt tccccaatac tgcatatatt ccccaaatcg
    24901 acacacggat atcagggcta tctcccacaa tataaaggtg cttttaccgt tttccggctg
    24961 cggtcaattg tgggacatcg cctataattt gcattatcat acctgtcatt caatgccgtg
    25021 cctaacacca ctgacgctat tcggacagga ttaacactag cgtagagatg acgagtacgt
    25081 tagtctctga ccgtttgtca cgcgcaacgt taccgatgat ggaacaataa aatcaacgta
    25141 tgaaaaaacg tatccccact ctcctggcca ccatgattgc caccgccctt tatagtcaac
    25201 agggactggc agccgacctc gcctcacagt gcatgttggg cgtgccaagc tatgaccgtc
    25261 ctctggtaca gggcgatacc aatgacttac ccgtgactat caatgctgac cacgcgaaag
    25321 gggactaccc ggatgacgcc gtgtttactg gcagcgtgga tatcatgcag ggtaacagcc
    25381 gtctgcaggc cgacgaagtg cagctccatc aaaaagaggc accaggacaa ccggagccgg
    25441 tacgtaccgt tgatgcgctc ggtaatgtcc attacgacga taaccaggtg atcctcaaag
    25501 ggccgaaagg ctgggcgaat ctgaacacca aagataccaa cgtctgggag ggtgattacc
    25561 agatggtggg tcgccagggc cgcggtaaag cggacctgat gaaacaacgt ggcgaaaacc
    25621 gctataccat tctggataac ggtagcttta cctcctgtct gccgggttct gacacctgga
    25681 gcgtggtagg tagcgaaatt attcatgacc gcgaagaaca agtggccgaa atctggaacg
    25741 cccgttttaa agttggcccg gtaccaatct tttatagccc ctatttgcag ttgccggtgg
    25801 gtgacaaacg tcgctctggt ttcttgatcc cgaacgccaa gtacaccacc accaactact
    25861 ttgagttcta cctgccatat tactggaaca tcgcgccaaa tatggatgcc accatcacgc
    25921 cgcattatat gcatcgtcgt ggcaacatca tgtgggagaa cgaattccgc tacctctccc
    25981 aggcgggcgc tggcttgatg gaactggact atctgccttc agataaagtc tatgaagatg
    26041 aacacccgaa cgatgacagt tcacgtcgtt ggttattcta ctggaaccac tccggggtca
    26101 tggatcaggt gtggcgtttc aacgtcgact acaccaaggt cagcgatcct agctacttca
    26161 atgatttcga taacaagtac ggttccagta ctgacggcta cgcaacgcaa aaattcagcg
    26221 ttggctatgc ggtgcaaaac ttcaatgcca ccgtttcaac caagcagttc caggtcttca
    26281 gcgaacagaa caccagtagc tactcggcag agccgcagtt agacgttaac tactaccaga
    26341 atgatgttgg tccgtttgat acgcgtattt acggccaggc agtgcacttt gttaacacca
    26401 gagacgacat gcctgaagca acccgtgttc acctggaacc gaccatcaat ttgccgctct
    26461 ctaataactg gggcagtatc aataccgaag cgaagttgct ggcaacccat tatcagcaaa
    26521 ctaatcttga ctggtataac tccagaaaca cgaccaagct ggacgaatcc gttaaccgcg
    26581 taatgccgca attcaaagtt gacggcaaaa tggtctttga acgcgatatg gaaatgctgg
    26641 ctccgggtta tacccaaacg ctggaaccgc gcgcgcagta tttgtacgtg ccatatcgcg
    26701 atcagagcga catctataac tacgactcat ctctgctgca atctgactac tctggcctgt
    26761 tccgggaccg gacttacggc ggtcttgacc gtattgcctc cgctaaccag gtgacgaccg
    26821 gtgtcacatc tcgcatatat gatgatgctg ccgttgaacg ttttaatatt tccgttggtc
    26881 aaatctacta tttcacggag tctcgcactg gcgatgacaa cataacatgg gagaatgacg
    26941 acaaaacggg ctcactggtg tgggcaggcg atacttactg gcgtatctcc gagcgttggg
    27001 gattgcgtgg cgggattcag tacgatacac gtctggataa cgtagcgacc agtaactcca
    27061 gcattgaata ccgtcgggat gaagaccgtc tggtacagct gaattaccgt tacgccagcc
    27121 cggaatatat tcaggctacg ctgcctaagt actattccac tgctgagcaa tataagaatg
    27181 gtatttcgca ggtaggtgct gtcgccagct ggccaattgc cgatcgttgg tccattgttg
    27241 gggcctacta ctacgacacc aatgcgaaca agcaagccga ctctatgtta ggtgtgcaat
    27301 acagctcctg ctgctatgca attcgcgtcg gttacgagcg gaagctgaac ggttgggata
    27361 acgataaaca acatgcggta tatgacaacg caatcggctt taacatcgaa cttcgcggcc
    27421 tgagctccaa ttacggtctg ggtacgcaag agatgctgcg ttcgaacatt ctgccgtatc
    27481 aaaacacttt gtgatttgtt gatttaccac gtaatccgca gtgcggttaa ttgaaatgga
    27541 aaaagtatga agaactggaa aacgctgctt ctcggtatcg ccatgatcgc gaataccagt
    27601 ttcgctgccc cccaggtagt cgataaagtc gcagccgtcg tcaataacgg cgtcgtgctg
    27661 gaaagcgacg ttgatggatt aatgcagtcg gtaaaactga acgctgctca ggcaaggcag
    27721 cagcttcctg atgacgcgac gctgcgccac caaatcatgg aacgtttgat catggatcaa
    27781 atcatcctgc agatggggca gaaaatggga gtgaaaatct ccgatgagca gctggatcag
    27841 gcgattgcta acatcgcgaa acagaacaac atgacgctgg atcagatgcg cagccgtctg
    27901 gcctacgatg gtctgaacta caacacctat cgtaaccaga tccgcaaaga gatgattatc
    27961 tctgaagtgc gtaacaacga ggtgcgtcgt cgcatcacca tcctgccgca ggaagtcgaa
    28021 tccctggcgc agcaggtggg taaccaaaac gacgccagca ctgagctgaa cctgagccac
    28081 atcctgatcc cgctgccgga aaacccgacc tctgatcagg tgaacgaagc ggaaagccag
    28141 gcgcgcgcca ttgtcgatca ggcgcgtaac ggcgctgatt tcggtaagct ggcgattgct
    28201 cattctgccg accagcaggc gctgaacggc ggccagatgg gctggggccg tattcaggag
    28261 ttgccgggga tcttcgccca ggcattaagc accgcgaaga aaggcgacat tgttggtccg
    28321 attcgttccg gcgttggctt ccatattctg aaagttaacg accttcgcgg cgaaagcaaa
    28381 aatatctcgg tgaccgaagt tcatgctcgc catattctgc tgaaaccgtc gccgatcatg
    28441 actgacgaac aggcccgcgt gaaactggaa cagattgctg ctgatatcaa gagtggtaaa
    28501 acgacttttg ctgccgcagc gaaagagttc tcgcaggatc caggttctgc taaccagggc
    28561 ggcgatctcg gctgggctac accagatatt ttcgatccag ccttccgtga cgccctgacc
    28621 cgcctgaaca aaggtcaaat gagtgcaccg gttcactctt cattcggctg gcatttaatc
    28681 gaactgcttg atacccgtaa tgtcgataaa accgacgctg cgcagaaaga tcgtgcatac
    28741 cgcatgctga tgaaccgtaa gttctcggaa gaagcagcaa gctggatgca ggaacaacgt
    28801 gccagcgcct acgttaaaat cctgagcaac taatggttaa aacccaacgt gttgtgatca
    28861 ctcccggcga gcccgccggg attggcccgg acttagttgt ccagcttgca cagcgtgagt
    28921 ggccggtcga actggttgtt tgtgccgatg ccactctcct taccgaccgg gcagcgatac
    28981 tcggtttgcc gctcaccctc cgcccttatt cccccaactc ccctgcacaa ccgcaaactg
    29041 cgggcacatt aacgctactt cctgtcgcgc tacgagaatc tgtcactgcg gggcagttag
    29101 cggttgaaaa tgggcattac gtggtggaga cgctggcgcg agcgtgtgat ggctgtctga
    29161 acggtgaatt tgctgcgctg atcacaggcc ccgtgcataa aggcgtcatt aacgacgcag
    29221 gcattccgtt taccggtcat accgagtttt tcgaagagcg ttcgcaggcg aaaaaagtgg
    29281 tgatgatgct ggcgaccgaa gaacttcgcg tggcgctggc aacgacgcat ttaccgctgc
    29341 gcgatatcgc agacgctatc acccctgcac ttttgcacga agtgattgct attttgcatc
    29401 acgatttgcg gaccaaattt ggtattgccg aaccgcgcat tctggtctgt gggctgaatc
    29461 cgcacgcggg cgaaggcggt catatgggta cggaagagat agacaccatt attccggtgc
    29521 tcgacgaact gcgggcgcag gggatgaaac ttagcgggcc gctgcctgct gataccctgt
    29581 ttcagcctaa atatctcgat aacgccgacg cggtgctggc gatgtaccac gatcagggtc
    29641 ttcccgtgct aaaataccag ggcttcgggc gcggtgtgaa cattacgctg ggcctgccct
    29701 ttattcgcac atcagtggac cacggcaccg cgcttgaact ggcgggacgt ggcgaagccg
    29761 atgtcggcag ttttattacg gcgcttaatc tcgccatcaa aatgattgtt aacacccaat
    29821 gaataatcga gtccaccagg gccacttagc ccgtaaacgc ttcgggcaaa actttctcaa
    29881 cgatcagttc gtgatcgaca gcattgtttc tgccattaac ccgcagaagg gccaggcgat
    29941 ggtcgaaatc ggccccggtc tggctgcatt gaccgaaccg gtcggcgaac gtctggacca
    30001 gctgacggtt atcgaacttg accgcgatct ggcggcacgt ctgcaaacgc atccattctt
    30061 aggcccgaaa ctgacgattt atcagcagga tgcgatgacc tttaactttg gcgaactggc
    30121 cgagaaaatg ggtcagccgc tgcgtgtttt cggcaacctg ccttataaca tctccacgcc
    30181 gttgatgttc catctgttta gctatactga tgccattgcc gacatgcact ttatgttgca
    30241 aaaagaggtg gtgaatcgtc tggttgcagg accgaacagc aaagcgtatg gtcgattaag
    30301 cgtcatggcg caatactatt gcaatgtgat cccggtactg gaagtaccgc cgtcagcctt
    30361 tacaccacca cccaaagtgg attccgccgt cgtgcgcctg gttcctcatg caacgatgcc
    30421 tcacccggtt aaagatgttc gcgtgttgag ccgcatcacc accgaagcct ttaaccagcg
    30481 tcgtaaaacc attcgtaaca gcctcggcaa cctgtttagc gtcgaggtgt taacgggaat
    30541 ggggatcgac ccggcgatgc gagcggaaaa tatctctgtc gcgcaatatt gccagatggc
    30601 gaactatctg gcggagaacg cgcctttgca ggagagttaa cgatgatcaa ttcgccccga
    30661 gtgtgtattc aggttcaaag cgtctacatt gaggctcaat cttcacctga taatgaacgt
    30721 tacgtttttg cttataccgt aaccatacgc aatctggggc gagcgccagt gcagttgttg
    30781 gggcgttact ggctgatcac caatggcaat ggccgtgaaa ccgaagtcca gggcgaagga
    30841 gtggttggcg tccagccact tatcgcgcct ggcgaagagt accagtacac cagcggtgca
    30901 atcattgaaa cgccgctggg caccatgcag ggtcactacg aaatgatcga tgaaaatggc
    30961 gtccctttca gcatcgacat tcccgtattc cgactcgccg ttcccacact cattcattaa
    31021 aagaatatgg cgacatacct tattggcgac gttcatggtt gttacgatga actgatcgca
    31081 ttgctgcata aagtagaatt tacccctggg aaagataccc tctggctgac aggcgatctg
    31141 gtcgcgcgcg gcccgggttc gctggatgtt ctgcgctatg tgaaatcctt aggcgacagc
    31201 gtacgtctgg tgctgggcaa tcacgatctg catctgctgg cggtatttgc cgggatcagc
    31261 cgcaataaac cgaaagatcg cctgacaccg ctgctggaag cgccggatgc cgacgagctg
    31321 cttaactggc tgcgtcgcca gcctctgctg caaatcgacg aagagaaaaa gctggtgatg
    31381 gcccacgcag ggatcacgcc gcagtgggat ctacagaccg ccaaagagtg cgcgcgcgat
    31441 gtagaagcgg tgctgtcgag tgactcctat cccttctttc tcgacgctat gtacggcgat
    31501 atgccaaata actggtcacc ggaattgcgg gggctgggaa gattgcgttt tatcaccaac
    31561 gcctttaccc gtatgcgttt ttgctttcca aacggccaac tggatatgta cagcaaagaa
    31621 tcgccggaag aagcccctgc cccactgaaa ccgtggtttg cgattcctgg ccctgtcgct
    31681 gaagaataca gcatcgcctt tggtcactgg gcatcgctgg aaggcaaagg tacgccggaa
    31741 ggtatttacg cgctggatac cggctgctgc tggggtgggt cattaacctg cctgcgctgg
    31801 gaagataaac agtattttgt ccagccgtcg aaccggcata aggatttggg tgagggagag
    31861 gcggtcgcgt cttaaatccc gtcgagcata aaatctaatg ctcctttaat caacgttcgg
    31921 tgcgcgctgg catcgcaaaa tttcgttccc agcgcgttaa ctggaacagt tgcagtctca
    31981 tgcgtcatga gcacatattc tttgccgtct accagtaaaa ggattgggtt aagccgttct
    32041 ggtgggcgaa tacggctaaa tcgttcaata ggtgttaatg gaataacaat acggcgatta
    32101 atcacaccaa taatgtcgct ggtaacatcg ataacaaagg gaaacgcggc gttcctgctg
    32161 cgactgcggt ataccgtaaa ttgcatcgct agaagctccg gtactcatca gagaaacatc
    32221 cggtttcatc agccaattga tttaacgcag cgatagcttc tgcgttctct tcacgccaac
    32281 gtgttgctgc atgtttttta agttcagcat tgagcgcaac cgtaagggtg gcgctcatat
    32341 ttaagcctgc atcacgagcg cgattgacta actcacggtc gacggtgacg gtcacacttt
    32401 gtgtggtacg ttttgcagtc atgatatcgt gctcctgtac aatttatacg tctagtatac
    32461 acggatatct gcacgtatca gtgatgccgg aattctaata tacaaaatta ccgccgctcc
    32521 agaatctcaa agcaatagct atgagagttc tgcgcatcag cgtcgtggaa ttcgctgaat
    32581 accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc cacttctgcg
    32641 tcgatatgcg tcagatacag tttttgcgct tttgccagga actgctcata aacgcgaccg
    32701 ccgccaatca ccatgatttc tggtacgtca ccacacgccg cgatggcttc atccaccgac
    32761 ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat atttttgcgt
    32821 cctggcaacg gacgaccgat ggattcccag gtatggcggc ccataatcac gggtttattt
    32881 aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat ggcgttttcc
    32941 atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga gatttcccga
    33001 taaaaaaaat tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg tgcgaggatg
    33061 atgttgagga aaattttata ttctgctggc gagtccacgc tctccccctg gactcgccgc
    33121 attacaatga aacaaaaaca aacagttagc tgtaaagtgt gatttacgtc actctttatt
    33181 aggatgaggg tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta ccttcttctg
    33241 ttccctgcca gccatgtcgt tgaattaatg acagatgttc gcggtcctcg gtgatgatct
    33301 cacttaacat cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg tcattctcaa
    33361 ccattgccat ctcttccacc atctgaatat taaagcggcg gaacacatcg gcacgttctc
    33421 gcgcttcata cggccccaga cctaaacttt ccagcgccag acgcccggtt ttcagcgcac
    33481 cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg acgcaaacga atgtagtggt
    33541 cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa atgttctttc accatctccg
    33601 tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc gctttcgctg
    33661 ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc gtaaaacact ttcataccaa
    33721 atttacgcag cgtttcgata tggtccggat cgtgatcgag caccaccatt ttcaccccgc
    33781 tggagagcag taaacgtccg gtaatctgtc caaaacgacc gaatccggcg ataatcacgc
    33841 gcggctgttc ttcatcgatc tcatcggctt cacgcgcttc ctcagtagaa gattgctcaa
    33901 ggcgattgag gatcaccagc agaatcggcg ttgctgccat cgacagcgcc accgccaggg
    33961 tcagcgattt cgcccactcc ggctccagca cattcgccat ctgcgccgcg ccaaatacca
    34021 caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc tgtttatttg
    34081 gcacctgcaa cggtcgggca atcagccaca gcatggcgat tttgatgatg aggaaaccga
    34141 gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt gccaaagtct atcgacatgc
    34201 caacaccgat gaaaaacagc cccaacagca aacctttaaa cggttcgata tcgctttcca
    34261 gcgcatgacg gtattccgag cttgccagca gtacgcccgc cagaaacgcg cccatcgcca
    34321 tcgacaagcc gacctcttcc agcagcaaac caaagccaaa cacaaggaat aacgccacgg
    34381 cactaaacac ttcccgcaag ccagagcggg caacaaaacg cagcgccgga cgcgtaacat
    34441 agcgccccag caataccacc agtaccagcg cacccgccac ttttaacgcc gagagagcaa
    34501 atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag cggaatcatc gccaccagcg
    34561 ggatcgccgc aatatcctgg aacagcagca ccgcaaaggc gctgcgaccc atttgcgtca
    34621 ccatcagatt gcgttcattc atcgcctgca tggcaatcgc cgtagaggag agcgccagcg
    34681 tcatgccgat caattccgcg acctgccagc gcagcccaag taacatgcag aacagcccca
    34741 gcaggccgcc gcaaatcacc atctgtaatg cgccaccgcc gaacaccgcc gcacgcagct
    34801 tccacagcct ttgtggatcg agttcgaggc cgataataaa cagcatcagc accacaccaa
    34861 tttcagcaaa gtgcagaata gattcggcat cggtcaccag ccgcaacccc cacgggccaa
    34921 taatgcagcc tgcaatcagg tagccaagta ctgatcccag accgaggcgt accgcaatgg
    34981 gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg aaccagcgta tgactatcca
    35041 tgatgggcct cctgccattc cagcagacgt tgcttatagt ggcgcgcctg cccttcgagg
    35101 gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg gtggcagcca gttcagcccg
    35161 cagtagattg ccgtcgcctg tagcggctgc gacagcacat caaagcccgg atgcgcacca
    35221 atttcaaaat ggcttttccc gccgccggtc gtcaccgccc acagcaaatg tttgccatgc
    35281 agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaga aaactttatc gatccaaagt
    35341 ttaaggagcg gtggaatgct gtaccactgc atcgggtgct gccagacgat cagatcggcg
    35401 cgagacagcg cctcctgctc ggcggcaata tcgatattga agtcaggata gagttgataa
    35461 agagagcgaa tttcgacgcc atccagcgtc cttgcctgtt caagcatccg tttattcgca
    35521 tgggaatgat gcggatacgg atgcgcataa attatgagga tcatagttat tagcccgcgt
    35581 gtttacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt atagtcaata
    35641 atattgattc agaaaagaga gaaatctgaa caaattatcc cattctctgc ccgcggatcg
    35701 tcatattgct ggtctgcgcc agtgacattc ctcgagtctc cggggcaaac gctacggaaa
    35761 tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg ataccgtaat
    35821 tattgataaa tatcggcagt gcccaggtcg aaacaatggt gccaatacga cttaaggaca
    35881 taatcacgcc cacggcagag gcgcggatat ccgtcgggaa gagttcatta ggatagagcc
    35941 actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc atcactacca
    36001 gccagatccc catatccggg attagcccca aaaccgccag cgccagcgtc atcatggcaa
    36061 agctgccaat caacaatgga cgccgtccgg cagtgtttag ccacagcatc ggcggaatac
    36121 agccgagcat aaagaacagg ctaatcacca cattccctag tgccgcgttt ttgccaaccc
    36181 ccaatcccaa caaaccaacg atttgcgggc caaaggtgta aatggcgaac attgggatca
    36241 cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg cgattaaaca
    36301 gatcgcgaaa acgggtttgc tgcggctgct cttcctcgaa agccaccggt tcgccaaaca
    36361 gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaacagc cagcggggag
    36421 attctggcag ttcgaatcga ccaatcaaaa tcaacaaaca agggatcgcc gcgctaccca
    36481 gcatccagcg ccagccgcct tccacatcat aaagccagta gccgaccaga tcggcgcagg
    36541 tcgcgccgac ataccacatg gcggcgataa agctgatgga aaatgcccgc tgacgggtac
    36601 tggagaactc ggtgatcatt gaggtggcga tgggataatc tgcaccgatg acaatgccga
    36661 taagtacccg catcaccaac agttcgacgg gggatgaaac aaacatcgtc gccaccgata
    36721 tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact ttatcggaaa
    36781 tataaccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg cccagcaagc
    36841 caatccagtc agcgtccagt ttcagcgccg gcgtcagttg ctccagcgcc acgccaatca
    36901 ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa atgcggcggt
    36961 gaatagagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt tattcctgtt
    37021 ttttgcaatg agagtcagtg cggcgttacg tatcaaacca acatcagccg taacggaact
    37081 ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc agccccagaa
    37141 tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg ccgtcatcct
    37201 gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagggcc tgtttatcgg
    37261 catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta ttgacgccca
    37321 gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca tgccatcctt
    37381 gattagattg atgaagccca ctttcttacc gtggcggagg attttcttgc gcatcagttc
    37441 tggcgcgctg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca gttccgggta
    37501 gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca tacgcatatc
    37561 gcgcagcggg ccactctcaa gatgctgacg atattccgcg agtttttgct tactgaaatc
    37621 atcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg caatcgccag
    37681 atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat caccggcaat
    37741 caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg gcactacgtg
    37801 agcggaatat tccaccagct tgccacccgc gatcagcggt gcaacggccg gatgttgttt
    37861 gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat gcagaccaca
    37921 aaccagcccc agcgacaggg tgttttcatt ggtataaaga aaaccgccgc ccatcaggcc
    37981 atcagtaggt gatcccgcaa acaggcaagc cgcgccctga ttaccctgca actgaaaacg
    38041 gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg ccacatccgt
    38101 cggtttgacg cgttttgcca tccccaattt ttcggcaagg atggagttca ccccatcagc
    38161 aaggatcacc gttttcgctt caatcacatc gccatcggct tctacaccga cgactttgcc
    38221 atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcac ccgcttcttc
    38281 ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag aacgctggga
    38341 tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt ccgtcataaa
    38401 cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gagtcggcga aacctggaat
    38461 aatgtgttcc agactgtggg catagagacg cccgccggtg acgttcttgg cacctgcgga
    38521 attgccacgc tcgataacta acacttgcgc accttcgcgg gcgagcacca gtgcggcaac
    38581 cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg acataagagc
    38641 tccctgtcag cggcagggcc gtaatgcagc cctgccagag tggatcaacg cgctaaagct
    38701 gcggtcagcg ccggaaggat cttcacggcg tcgccaacaa tgccgtaatc cgcgtactgg
    38761 aagatcggcg catttttatc tttgttgatg gcgaaaatgg tttgcgatgc attagcacca
    38821 accatgtgct ggatctgccc ggagatcccc accgccaggt acagttcagg tttcagcatc
    38881 aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc cgccaccgga
    38941 cgagaacagg ccaactccgc acctatcgcc ttacaaagct gttctgccag cgcaatgttc
    39001 tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc gagatcgacg
    39061 ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg agcctgccac
    39121 tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc gaacgtgcca
    39181 ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat cgccaggccg
    39241 ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt gctggcatcg
    39301 ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt gccgcgacgg
    39361 gtgtttggca acagcaccag gccgtctgtg ccgtgctggc gaatagtgtc agccatgaca
    39421 ctggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca gacatgatta
    39481 gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggt gttgatttga
    39541 ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagaaggggt atcgctgaat
    39601 acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact ttgcgaagat
    39661 tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg cgctgacgct
    39721 cgcgctgttt cggcgcggca acctgttgtt ctgaccaggc tgcctctgcg ttaaaaccga
    39781 tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg gctttcatcg
    39841 aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc agcggaatgc
    39901 ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct gccgtcaggg
    39961 agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc accagcagac
    40021 caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga tcaaagcctg
    40081 ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc tgctcgaact
    40141 ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct ttacgccctt
    40201 tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc tctgctgcct
    40261 gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt attttggcat
    40321 cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc tgttcatcag
    40381 gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg aaggaaacgt
    40441 cgttcatatc ggtggtgtta attaca
//
LOCUS       DAZKFU020000011        37888 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZKFU020000011 DAZKFU020000000
VERSION     DAZKFU020000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 37888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.011"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            165..2408
                     /gene="pdeF"
                     /locus_tag="ACHOQQ_RS02325"
                     /old_locus_tag="pgaptmp_000465"
     CDS             165..2408
                     /gene="pdeF"
                     /locus_tag="ACHOQQ_RS02325"
                     /old_locus_tag="pgaptmp_000465"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGU4468770.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2447..3988)
                     /gene="ppx"
                     /locus_tag="ACHOQQ_RS02330"
                     /old_locus_tag="pgaptmp_000466"
     CDS             complement(2447..3988)
                     /gene="ppx"
                     /locus_tag="ACHOQQ_RS02330"
                     /old_locus_tag="pgaptmp_000466"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU4468771.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3993..6059)
                     /gene="ppk1"
                     /locus_tag="ACHOQQ_RS02335"
                     /old_locus_tag="pgaptmp_000467"
     CDS             complement(3993..6059)
                     /gene="ppk1"
                     /locus_tag="ACHOQQ_RS02335"
                     /old_locus_tag="pgaptmp_000467"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGU4468772.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6230..6868)
                     /gene="purN"
                     /locus_tag="ACHOQQ_RS02340"
                     /old_locus_tag="pgaptmp_000468"
     CDS             complement(6230..6868)
                     /gene="purN"
                     /locus_tag="ACHOQQ_RS02340"
                     /old_locus_tag="pgaptmp_000468"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGU4468773.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6868..7905)
                     /gene="purM"
                     /locus_tag="ACHOQQ_RS02345"
                     /old_locus_tag="pgaptmp_000469"
     CDS             complement(6868..7905)
                     /gene="purM"
                     /locus_tag="ACHOQQ_RS02345"
                     /old_locus_tag="pgaptmp_000469"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGU4468774.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8230..8856
                     /gene="upp"
                     /locus_tag="ACHOQQ_RS02350"
                     /old_locus_tag="pgaptmp_000470"
     CDS             8230..8856
                     /gene="upp"
                     /locus_tag="ACHOQQ_RS02350"
                     /old_locus_tag="pgaptmp_000470"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGU4468775.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8942..10231
                     /gene="uraA"
                     /locus_tag="ACHOQQ_RS02355"
                     /old_locus_tag="pgaptmp_000471"
     CDS             8942..10231
                     /gene="uraA"
                     /locus_tag="ACHOQQ_RS02355"
                     /old_locus_tag="pgaptmp_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGU4468776.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10326..11027
                     /gene="hda"
                     /locus_tag="ACHOQQ_RS02360"
                     /old_locus_tag="pgaptmp_000472"
     CDS             10326..11027
                     /gene="hda"
                     /locus_tag="ACHOQQ_RS02360"
                     /old_locus_tag="pgaptmp_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGU4468777.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11165..11524)
                     /gene="arsC"
                     /locus_tag="ACHOQQ_RS02365"
                     /old_locus_tag="pgaptmp_000473"
     CDS             complement(11165..11524)
                     /gene="arsC"
                     /locus_tag="ACHOQQ_RS02365"
                     /old_locus_tag="pgaptmp_000473"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGU4468778.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11545..13008)
                     /gene="bepA"
                     /locus_tag="ACHOQQ_RS02370"
                     /old_locus_tag="pgaptmp_000474"
     CDS             complement(11545..13008)
                     /gene="bepA"
                     /locus_tag="ACHOQQ_RS02370"
                     /old_locus_tag="pgaptmp_000474"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGU4468779.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13221..14282
                     /gene="yfgO"
                     /locus_tag="ACHOQQ_RS02375"
                     /old_locus_tag="pgaptmp_000475"
     CDS             13221..14282
                     /gene="yfgO"
                     /locus_tag="ACHOQQ_RS02375"
                     /old_locus_tag="pgaptmp_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4468780.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVIGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14320..15168)
                     /gene="focB"
                     /locus_tag="ACHOQQ_RS02380"
                     /old_locus_tag="pgaptmp_000476"
     CDS             complement(14320..15168)
                     /gene="focB"
                     /locus_tag="ACHOQQ_RS02380"
                     /old_locus_tag="pgaptmp_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HGU4468781.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15190..17202)
                     /gene="hyfR"
                     /locus_tag="ACHOQQ_RS02385"
                     /old_locus_tag="pgaptmp_000477"
     CDS             complement(15190..17202)
                     /gene="hyfR"
                     /locus_tag="ACHOQQ_RS02385"
                     /old_locus_tag="pgaptmp_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HGU4468782.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLKKDCDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLIQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17232..17645)
                     /gene="hyfJ"
                     /locus_tag="ACHOQQ_RS02390"
                     /old_locus_tag="pgaptmp_000478"
     CDS             complement(17232..17645)
                     /gene="hyfJ"
                     /locus_tag="ACHOQQ_RS02390"
                     /old_locus_tag="pgaptmp_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGU4468783.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFCCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDTSHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17638..18396)
                     /gene="hyfI"
                     /locus_tag="ACHOQQ_RS02395"
                     /old_locus_tag="pgaptmp_000479"
     CDS             complement(17638..18396)
                     /gene="hyfI"
                     /locus_tag="ACHOQQ_RS02395"
                     /old_locus_tag="pgaptmp_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HGU4468784.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDN
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLTGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(18393..18938)
                     /gene="hyfH"
                     /locus_tag="ACHOQQ_RS02400"
                     /old_locus_tag="pgaptmp_000480"
     CDS             complement(18393..18938)
                     /gene="hyfH"
                     /locus_tag="ACHOQQ_RS02400"
                     /old_locus_tag="pgaptmp_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGU4468785.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18948..20663)
                     /gene="hyfG"
                     /locus_tag="ACHOQQ_RS02405"
                     /old_locus_tag="pgaptmp_000481"
     CDS             complement(18948..20663)
                     /gene="hyfG"
                     /locus_tag="ACHOQQ_RS02405"
                     /old_locus_tag="pgaptmp_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGU4468786.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTLEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGDSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALRIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20653..22233)
                     /gene="hyfF"
                     /locus_tag="ACHOQQ_RS02410"
                     /old_locus_tag="pgaptmp_000482"
     CDS             complement(20653..22233)
                     /gene="hyfF"
                     /locus_tag="ACHOQQ_RS02410"
                     /old_locus_tag="pgaptmp_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGU4468787.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRAVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGELGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22238..22888)
                     /gene="hyfE"
                     /locus_tag="ACHOQQ_RS02415"
                     /old_locus_tag="pgaptmp_000483"
     CDS             complement(22238..22888)
                     /gene="hyfE"
                     /locus_tag="ACHOQQ_RS02415"
                     /old_locus_tag="pgaptmp_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGU4468788.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22900..24339)
                     /gene="hyfD"
                     /locus_tag="ACHOQQ_RS02420"
                     /old_locus_tag="pgaptmp_000484"
     CDS             complement(22900..24339)
                     /gene="hyfD"
                     /locus_tag="ACHOQQ_RS02420"
                     /old_locus_tag="pgaptmp_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HGU4468789.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24356..25303)
                     /gene="hyfC"
                     /locus_tag="ACHOQQ_RS02425"
                     /old_locus_tag="pgaptmp_000485"
     CDS             complement(24356..25303)
                     /gene="hyfC"
                     /locus_tag="ACHOQQ_RS02425"
                     /old_locus_tag="pgaptmp_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGU4468790.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25314..27332)
                     /gene="hyfB"
                     /locus_tag="ACHOQQ_RS02430"
                     /old_locus_tag="pgaptmp_000486"
     CDS             complement(25314..27332)
                     /gene="hyfB"
                     /locus_tag="ACHOQQ_RS02430"
                     /old_locus_tag="pgaptmp_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HGU4468791.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGTISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYLVAALVVLLIAIAV"
     gene            complement(27332..27949)
                     /gene="hyfA"
                     /locus_tag="ACHOQQ_RS02435"
                     /old_locus_tag="pgaptmp_000487"
     CDS             complement(27332..27949)
                     /gene="hyfA"
                     /locus_tag="ACHOQQ_RS02435"
                     /old_locus_tag="pgaptmp_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGU4468792.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNENLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPQGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28202..28672)
                     /gene="bcp"
                     /locus_tag="ACHOQQ_RS02440"
                     /old_locus_tag="pgaptmp_000488"
     CDS             complement(28202..28672)
                     /gene="bcp"
                     /locus_tag="ACHOQQ_RS02440"
                     /old_locus_tag="pgaptmp_000488"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGU4468793.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FDVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28672..29244)
                     /gene="gcvR"
                     /locus_tag="ACHOQQ_RS02445"
                     /old_locus_tag="pgaptmp_000489"
     CDS             complement(28672..29244)
                     /gene="gcvR"
                     /locus_tag="ACHOQQ_RS02445"
                     /old_locus_tag="pgaptmp_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGU4468794.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29390..30268
                     /gene="dapA"
                     /locus_tag="ACHOQQ_RS02450"
                     /old_locus_tag="pgaptmp_000490"
     CDS             29390..30268
                     /gene="dapA"
                     /locus_tag="ACHOQQ_RS02450"
                     /old_locus_tag="pgaptmp_000490"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGU4468795.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30285..31319
                     /gene="bamC"
                     /locus_tag="ACHOQQ_RS02455"
                     /old_locus_tag="pgaptmp_000491"
     CDS             30285..31319
                     /gene="bamC"
                     /locus_tag="ACHOQQ_RS02455"
                     /old_locus_tag="pgaptmp_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGU4468796.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31532..32245
                     /gene="purC"
                     /locus_tag="ACHOQQ_RS02460"
                     /old_locus_tag="pgaptmp_000492"
     CDS             31532..32245
                     /gene="purC"
                     /locus_tag="ACHOQQ_RS02460"
                     /old_locus_tag="pgaptmp_000492"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGU4468797.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32413..33276
                     /gene="ypfJ"
                     /locus_tag="ACHOQQ_RS02465"
                     /old_locus_tag="pgaptmp_000493"
     CDS             32413..33276
                     /gene="ypfJ"
                     /locus_tag="ACHOQQ_RS02465"
                     /old_locus_tag="pgaptmp_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGU4468798.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33291..35306
                     /gene="tmcA"
                     /locus_tag="ACHOQQ_RS02470"
                     /old_locus_tag="pgaptmp_000494"
     CDS             33291..35306
                     /gene="tmcA"
                     /locus_tag="ACHOQQ_RS02470"
                     /old_locus_tag="pgaptmp_000494"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGU4468799.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARYGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSNAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35380..36078
                     /gene="ypfH"
                     /locus_tag="ACHOQQ_RS02475"
                     /old_locus_tag="pgaptmp_000495"
     CDS             35380..36078
                     /gene="ypfH"
                     /locus_tag="ACHOQQ_RS02475"
                     /old_locus_tag="pgaptmp_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4468800.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36159..36359)
                     /gene="ypfN"
                     /locus_tag="ACHOQQ_RS02480"
                     /old_locus_tag="pgaptmp_000496"
     CDS             complement(36159..36359)
                     /gene="ypfN"
                     /locus_tag="ACHOQQ_RS02480"
                     /old_locus_tag="pgaptmp_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGU4468801.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36387..37514)
                     /gene="dapE"
                     /locus_tag="ACHOQQ_RS02485"
                     /old_locus_tag="pgaptmp_000497"
     CDS             complement(36387..37514)
                     /gene="dapE"
                     /locus_tag="ACHOQQ_RS02485"
                     /old_locus_tag="pgaptmp_000497"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGU4468802.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37518..37874)
                     /gene="yffB"
                     /locus_tag="ACHOQQ_RS02490"
                     /old_locus_tag="pgaptmp_000498"
     CDS             complement(37518..37874)
                     /gene="yffB"
                     /locus_tag="ACHOQQ_RS02490"
                     /old_locus_tag="pgaptmp_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGU4468803.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9217 a         9820 c         9972 g         8879 t
ORIGIN      
        1 ttcattaatc agggttattt tgtttgatat atatcaattg gaactttcat atgacactta
       61 gaatcaatgc tctctatagt gatgaataat catcattcga agtcaggtgg gatgcctgtc
      121 tgaatacacc tccttcagga tgtgggggat tctgctgagc atctatgaaa ctgaatgcaa
      181 cttatataaa aatacgtgat aaatggtggg ggcttccgct gttcctgcct tctttaatct
      241 tgcccatttt cgcccacatt aatactttcg cgcatatttc ctccggtgag gtttttctct
      301 tttatctgcc tttggcactg atgatcagca tgatgatgtt tttcagctgg gcggcattgc
      361 cagggatcgc cttagggatt tttgtccgca aatatgcaga gcttggtttt tacgaaacgc
      421 tctcattaac ggctaatttt attatcatta tcattctctg ttggggcggt tacagggtct
      481 ttactccccg gcgtaacaac gtttcacatg gtgatacccg tttaatttcc cagcgtatat
      541 tctggcagat tgtgtttcct gcaacgctgt ttctgatact tttccagttt gctgcatttg
      601 taggattact ggcgagcaga gaaaatctgg ttggcgtcat gccttttaat ctcgggacct
      661 taatcaatta tcaggcctta ctggtgggca atctgattgg tgtcccgctg tgttacttca
      721 tcattcgggt agtgcgaaat ccgttttatt tacgtagtta ttattcgcaa ttaaaacagc
      781 aggttgatgt caaagtcacc aaaaaagagt tcgcactctg gctactggca ttaggtgctt
      841 tactgttgct gttatgcatg ccgttaaatg aaaaaagcac aatttttagc accaattata
      901 ccttgtcatt attgctgccc ctgatgatgt ggggagcgat gcgctatggt tataagctga
      961 tttcactgct ctgggcggtc gtgttgatga tcagcatcca cagctatcaa aattacattc
     1021 ccatttatcc cggctatacc acgcagctga ccataacctc ctccagttat ctggtattct
     1081 cttttattgt caattatatg gctgtactgg caacccgtca gagaacggta gtcagacgca
     1141 ttcagcggct tgcgtatgtg gacccggtgg ttcatctgcc aaatgttcgc gccctgaatc
     1201 gcgcgttgcg tgatgccccc tggtctgcgc tttgttattt acgcatccct ggcatggaaa
     1261 tgctggttaa aaactatggc atcatgctgc ggattcaata caagcaaaaa ctttctcact
     1321 ggctgtcacc attgctggaa ccgggtgaag atgtttatca gctttcgggt aacgatctcg
     1381 cgctgcgact gaatacagaa tcgcaccagg agcgcattac cgcactggat agccatctca
     1441 agcaatttcg tttcttttgg gatggcatgc cgatgcaacc gcagattggc gtcagttact
     1501 gctatgtgcg ctcgccagtg aatcatatct acctgctgct gggagagcta aatacggtcg
     1561 ccgaactttc catcgtgacc aacgccccgg aaaatatgca gcgtcgcggg gcaatgtatt
     1621 tgcaacgcga attgaaagat aaagtcgcga tgatgaatcg actacagcag gcgctggaac
     1681 acaaccattt tttcctgatg gcccagccga ttaccggtat gcgtggtgat gtttaccatg
     1741 aaattcttct gcgcatgaaa ggtgagaatg atgaactgat cagccccgat agcttcttgc
     1801 cggtcgcgca cgaatttggt ttatcgtcga gtatcgacat gtgggtcatt gagcatacgc
     1861 tgcaatttat ggctgaaaac agagcgaaga tgcccgctca ccgttttgct attaatctgt
     1921 ctccaacctc ggtatgtcag gctcgttttc ctgttgaagt cagtcagttg ctggctaaat
     1981 atcagattga agcgtggcaa cttatttttg aagtcaccga aagtaatgct ctgaccaatg
     2041 ttaagcaggc gcaaatcacc ttgcagcatc ttcaggaatt aggctgccag attgcgattg
     2101 atgatttcgg caccggctac gccagctatg cgcggcttaa aaatgtgaat gccgatctgc
     2161 ttaaaattga cggcagtttt atccgcaata ttgtgtcaaa tagtctggat tatcagatag
     2221 tggcatcgat ttgccacctg gcgcgaatga agaaaatgcg ggtagtggca gagtacgttg
     2281 aaaacgaaga gatccgcgag gcagtgctct ctttggggat cgattatatg cagggttatc
     2341 ttattggtaa gccgcaaccg ttaattgata cgctgaatga aatcgaaccc attcgcgaaa
     2401 gtgcctgaat aatgcgggcc gacatttctc gtcggcccgc aaagcattaa gcggcgattt
     2461 caggtgtact ttcttcttca attttcaacc gccagccagc cacgccttcc cagtattctt
     2521 gctccttttc cagatcaagc agtaccaacg cattctgact aaaccagtca tgcgggaaac
     2581 gcagtgtcca gtgactgtca tcagtaatca gtgtcaatgt tggcggtgtg gtggttgcct
     2641 gacgttgatt gttgagtaat acgccaaggc gcaatagctg aatcagtggc aggaactgtt
     2701 tcttcttaaa caaggtaaag cgcggcagat cgtcgagttt aatcgcttta cggtgatagc
     2761 gcaccagtgt cgccatcatc agctgctgtt cctgattaaa acccggcaag tcgctgtttt
     2821 gtagaatata agcggagtga cgatgcaaac cgctgtggtt gatattcaac ccgacctcat
     2881 gcagcatggc ggcccatcgc agtagcgcct ccagttgcgg atgcgccagc ttcggttgct
     2941 gttcccgcca ctgttcgtac atttgcatag tggtatccag cacccgtcgg gcctgttcgc
     3001 tgtcgatgtg atactggttg gcgaggctgc tggcggtgcg actacgcaca tcctgatgac
     3061 ggaaacgtcc ttccatttca tacagtacgc cttcgcgtaa cgccccgtca gaaaggcgca
     3121 gttcacggat ggctaaagca tcaaacacac cgcataaaat cgccagtccc ggaacgaaga
     3181 ctgttttccg ctcttcggaa agacctggta aactcagcga tgcgaaatta cggtgacgta
     3241 aaacttcttt taccagtttt tccagacgtt ccggggtaat tatcccgtct ttctcgccca
     3301 tttccattaa tacttcatgg gcggctttta tggtaccgga agcgcccatt gccacgttcc
     3361 agccctgaat acggaattgc caggttaaag tttccagttt ttgtgctgcc gccatgcgag
     3421 cacgctgaaa attctcttta ttgatgactc cgccagggaa ataaagctgg gcaaagctga
     3481 cacaccccat ccggcggctt tcaacgagaa tcggttcgaa attttcacca atcaccagtt
     3541 ctgtagaacc gccgccaata tcaataacca gtttgcgacc tttttccggt tgggtatgtt
     3601 ccacgcccat aaaaatcaga cgggcttctt cattaccgga aataatttca atcgggtagg
     3661 gaatgacctt ttccgcacgt ttcagaaagt cagtggcgtt cagcgcctga cgcagcgtat
     3721 gggtaccaac tatacagacg ctggcaggag aaaacccttg tagccgttcg gcaaacagcg
     3781 acagacagtt caaaccgcgc gtcattgcct cttcactcaa catattatct ggccccaggc
     3841 cgtccgccag atgcacccgc tgtttcaggc ggccaataat ctgcatggca ccatctacca
     3901 cacgggctat gaccatgtga aaactgtttg aaccaagatc gactgcagca aactcctgcg
     3961 gacgagggga tttatcgtgt attggcatag ggttattcag gttgttcgag tgatttgatg
     4021 tagtcataaa tcgccaactg cgcccgtact ttgcggcgat tgccgcgggg aacgtagcga
     4081 ttactgagtt ctttatcgat ataacgtgct ttgaccgtat cgctgaacaa tatgtcgatg
     4141 atgtccagca cccgctgctt caggcgcgga tcgagcagcg gcgtcgccac ttcaatacga
     4201 taatcaatat tgcgcgtcat ccagtcggcg gaagaaaggt agaccttttt atcgccgcca
     4261 ttttcaaaaa tataaacccg gtcatgttca aggtaacggt caacaatact gatggcacga
     4321 atgttgtcgc taatgccttc cagattgggg atcagcgaac acattccgcg aaccagcaga
     4381 ttaaccggta cgccggagct ggaggccgca tacagacgat caaccaggcc tttatcgaca
     4441 aggttattta gcttcagggt gataccactg ggcagccctt gctgcgcgtt ggcgatctcg
     4501 cggtccacca tttcatacaa caggcggcgg gagttttgcg gcgacaccat taaataatca
     4561 aatgtcaccg gacggtatgg gttttcaata aagttgaaca cccgccgtac ttcgttagtg
     4621 atgcgcgcat cggcggtcag caacgaatag tcagtataaa gacgcgcggt tttttcgtta
     4681 aagtttccgg tcccaatgtg tgcgtaacgc accacttcac cgttttcttt acgtgaaatc
     4741 aggaacagtt tggcgtgaat tttcagcccc ggcgcagaga agataacgtg cacgcctgct
     4801 tcggtcaggc gcttcgccca gtgaatgttt gcttcttcgt cgaaacgcgc ctgtaactca
     4861 accaccacgg tgactttctt accgttgtgt gcggcgtgga tcatcgagtc gatgatgcgt
     4921 gaatctttcg ccacgcggta aatgttaatt ttaatcgcca gtacgctcgg gtcgaacgaa
     4981 gcctgacgca gcagttccag cacatgctca aaggtgtgat aaggataata gagcaacaca
     5041 tcgcgttcgc gaatggcatc aaaaccattg cggaactggg ctttatcaaa ccaaatgtgg
     5101 cgtaaacgcg gcagtggttt gttcaccaga ttggctttgc cgacattggg gaaattaata
     5161 aagtctttaa aattatgata acgaccgccg gggacgatgg agtcgtagcg ggaaatagtc
     5221 agtttttcgc gtaacacttc aaccagcgca ttgggcatat cgcgctgata aacaaaacgc
     5281 accggctcag cagttaaacg ctgcttgaga ctggaagaca tcaactccat caggctggct
     5341 tccatctcat gcactaaatc gtattcggca tcgcgggtca tcttcattga ataggcattc
     5401 agcgcgtcat aatcaaagaa gcctttgaaa atatcatcaa ggcagtaacg cagaatgtta
     5461 tccagaagaa tcatcggctt gcgtcgacgc ggcgcttctg gcggtaaatt cacaaagcgc
     5521 ggcactttat ctgatgggat ctccagcagc gcgtaacgga tggtatcgcc acggataatt
     5581 tccaccgcca gataggtgta atcatctttc aggaactgca ctaagtcagt gtcaggattg
     5641 attaaaatcg gcgtaatgtg ctgacgcaga tactgcttaa aataatgacg cagccagttt
     5701 tgttgattga cggagagctg gcgttcatta atcaggaaga tctggttgcg cgccatctcc
     5761 agcaatagct cgttgtagag gccgtcgaat tcctgatcgg ctttcagcac ccgggactga
     5821 attttgccca gtaaatggcg ggaatgagag ttggagcctt gttcttcgct aataatgatg
     5881 cgtcgcttca gttcagcgaa gcggacttta tagaactcat caaggttatt ggaatagatc
     5941 cccaggaaac gcatcctttc aatcagcggg ttagatttgt ccgccgcttc ctgaagcacg
     6001 cgttcattga acgataacca actgagctct ttttcgatgt atagcttttc ctgacccatt
     6061 accactttta ctccgtttta ttcacgggac actgcttgga tattatggcg agcaatattg
     6121 gcagatgtcc aactatgcca gaaaaggggg aaaaaaactg ctggagcttg cgatttacag
     6181 gtgaattgaa aaacgccagc ggcagagctg gcgctttaac tacggggcat tactcgtcgg
     6241 cagcgtagcc ctgcggcggc agacgttgac catccagcca cgcggcgttt tcgtgcattt
     6301 tcagacgacc atcggcaaac cagctaatca ccagtggata aatggcgtgt tcctgggttt
     6361 gcacgcgggc ggtgacgtca tcttccgtat caccagcaaa taccgggact ttcgcctgta
     6421 aaataaccgg gccaccgtcc agttcatcgg tgacgaaatg caccgatgta ccgtgctctt
     6481 catcgccatt ttccagcgcc tgacgatggg tgtgtaatcc gggatatttc ggcagcagag
     6541 aagggtgaat gttcagcaaa cgcccggcat agtgggagac aaacgccggg ctgagaatgc
     6601 gcataaaacc agccagcacg accacatcgg gtgcgtacat gtcgatttca tgaatcaact
     6661 cccggtcata ggcttcacga ctgtcaaacg cgctggcgat gagcgtatgc gttgcaatac
     6721 ccgcctggcg ggcgcgttca aggccgaacg cgtcggcctt attgctgaaa actgcccgta
     6781 cggtgccttt aattttgttg gttttacagg cgtcaataat tgcctgtaaa ttacttccgt
     6841 tgccggaaat aagcaccaca atattcatta ttcgataacc acgcgttgtt cggaatcaga
     6901 ggctttgatg ataccgattt tccacgcgtt ttcaccgttg gcattgagca gggcgagggc
     6961 tttgtccact tccggagcag gcagggcgat aatcatcccg acgccgcagt tgaaggtgcg
     7021 atacatttca tggcgctcaa cgttaccagc cgtttgcagc cagttgaaca cttccggcca
     7081 ctgccaggag gattcatcaa tcactgcctg ggtattatct ggcaatacgc gcggaatgtt
     7141 ttcccagaag ccgccgccgg tcaggtgcgc aatggcatgc acatcgacct tttcaatcaa
     7201 ctccagcact gacttcacgt aaatgcgggt cggtgccagc agatgatcgg ctaatggctt
     7261 accatcaagt tcggtggttt gcggatcaca accgctgact tcaagaattt tgcgcaccag
     7321 cgaataaccg ttcgagtgcg gaccgctgga accgagtgca atcagcacat cgccgtcgct
     7381 gactttagag ccgtcgatga tttctgattt ttctaccacg cccacgcaga aacccgcgac
     7441 atcgtaatcc tcaccgtgat acatccccgg catttctgcc gtttcgccac ccaccagtga
     7501 acagcccgat tgcagacaac cttccgcaat gccgctgatc accgctgaag cggtatcaac
     7561 atccagtttt ccggttgcgt aatagtcgag gaaaaacagc ggttctgcac cttgcaccac
     7621 caggtcatta acgcacatgg cgaccagatc aataccaatg gtgtcgtgac gttttaagtc
     7681 cattgccaga cgcagcttgg tacctacgcc gtcagtgccg gaaaccagca cgggttcacg
     7741 atatttttgc ggcaatgcac acagcgcacc gaagccgccc agaccgccca tcacttccgg
     7801 acgacgcgtt ttcttcacta cgcctttgat tcttccaacc agagcattac ccgcgtcaat
     7861 atcaacaccg gcatctttgt agctaagaga ggttttatcg gtcactgctt gggtccccac
     7921 gcgttacttg cggtagaaaa ataaaattcg gcgcaattct aacagggaaa gcaaacgttt
     7981 gcgagactgc tttacacaac ctttttgcac gtcttttccc caggcgcgcg gcgaaagaag
     8041 acttgtgcca gggcaaaggt tagttttcgg atggaataat cttctttcat aaccatttga
     8101 atataaaata actttatctc aaaccgttat cattttgact aaagtcaacg aaaataatat
     8161 tgccgccttg aagaaaggag gtataatccg tcgatttttt ttgtggctgc ccctcaaagg
     8221 agaaagagta tgaagatcgt ggaagtcaaa cacccactcg tcaaacacaa gctgggactg
     8281 atgcgtgagc aagatatcag caccaagcgc tttcgcgaac tcgcttccga agtgggtagc
     8341 ctgctgactt acgaagcgac cgccgacctc gaaacggaaa aagtaactat cgaaggctgg
     8401 aacggcccgg tagaaatcga ccagatcaaa ggtaagaaaa ttaccgttgt gccaattctg
     8461 cgtgcgggtc tggggatgat ggacggtgtg ctggaaaacg ttccgagcgc gcgcatcagc
     8521 gttgtcggta tgtaccgtaa tgaagaaacg ctggagccgg taccgtactt ccagaaactg
     8581 gtttctaaca tcgatgagcg tatggcgctg atcgttgacc caatgctggc aaccggtggt
     8641 tccgttatcg cgaccatcga cctgctgaaa aaagcaggct gcagcagcat caaagttctg
     8701 gtactggtag ctgcgccaga aggtatcgct gcgctggaaa aagcgcaccc ggacgtcgaa
     8761 ctgtataccg catcgattga ccagggactg aacgagcacg gatacattat tccgggcctc
     8821 ggcgatgccg gtgataaaat ctttggtacg aaataaagaa taaaaataat taaagccgac
     8881 tttaagagtc ggcttttttt tgagtaaagc gcctataaca cataatacag aggataatac
     8941 tatgacgcgc cgtgctatcg gggtgagtga aagaccgcca cttttacaga caatcccgct
     9001 tagtttgcaa catttgttcg ccatgtttgg tgcaaccgtc ctggtgcccg tcttatttca
     9061 tattaacccg gcgactgtac tgttatttaa cggtattgga acgctgctgt atctcttcat
     9121 ctgtaaaggg aaaattccgg cttatcttgg ttccagcttt gcctttattt caccggtatt
     9181 gttactgttg ccgttagggt atgaagtcgc gctgggcggc tttattatgt gcggcgtgct
     9241 gttctgcctg gtttctttta tcgtgaagaa agcggggacc ggctggctgg acgtgctgtt
     9301 tccacctgcg gcaatgggcg caatcgttgc cgtcatcggt ctggagctgg cgggcgtagc
     9361 tgccggtatg gcgggtttac tcccggctga agggcaaacg ccagactcca aaaccatcat
     9421 catctctatt accaccctgg cggtcacggt tttaggttcc gtgctgtttc gtggtttcct
     9481 ggcaattatc ccgattttaa ttggcgtgct ggtggggtac gcgctctctt tcgcaatggg
     9541 aattgtcgat accacgccga ttattaatgc tcactggttt gcgctgccaa ccctctatac
     9601 gccgcgcttc gagtggtttg ccattctgac tattctgcca gcggcgttag tggttattgc
     9661 cgaacacgta gggcacctgg tagtaacggc taatatcgtc aaaaaagatc tgctgcgcga
     9721 tccaggtctg caccgttcga tgtttgctaa cggcctgtcg accgtgatct ccggcttctt
     9781 tggctctacg ccaaatacca cttacggaga aaacattggc gtgatggcga tcacccgtgt
     9841 ttacagtacc tgggttatcg gcggggcggc gattttcgct atcctgcttt cctgcgtcgg
     9901 taaactggct gctgctatcc agatgatccc attgccggtg atgggtggtg tttcgctgct
     9961 gctttacggt gtcatcggtg cttccggtat tcgtgtgttg atcgaatcga aagtggatta
    10021 caacaaagca cagaacctga tcctgacttc cgtgattttg atcatcggcg tcagtggcgc
    10081 gaaggtaaac attggcgcgg cggaactgaa aggtatggcg ctggcgacca tcgtcggtat
    10141 cggcttaagt ttgattttca aactgattag tgtgctgcgc cctgaagaag tggtgctgga
    10201 cgcagaagac gcggatataa cagacaaata atgtaccgac cgggcagtgt tgctgcccgg
    10261 ttcaaacatc atgggattct gtggtaaact tctcgcgatt ttgtgaaatc ctggttgagg
    10321 tatctctgaa cacaccggca cagctctctt tgccacttta tcttcctgac gacgaaacct
    10381 ttgcaagttt ctggccgggg gataactcct ctttactggc cgcgctgcaa aacgtgctgc
    10441 gtcaggaaca tagcggttac atctatctct gggcacgcga aggcgcgggg cgcagccatc
    10501 tgctgcacgc ggcttgcgcg gaattgtcgc agcgtggcga tgcggtgggc tatgtcccgc
    10561 tggataaacg cacctggttt gttccggaag tgctcgacgg tatggagcat ttgtcgctgg
    10621 tctgtatcga caacattgag tgtattgcag gcgatgagtt gtgggagatg gcgattttcg
    10681 atctctacaa tcgaattctg gaatcgggca aaacacggtt gttgatcacc ggcgatcgtc
    10741 caccgcggca gttgaatctg ggattaccgg atctcgcgtc gcgactcgac tgggggcaga
    10801 tctacaaatt gcagccactt tctgatgaag ataagttgca ggcgctacag ttacgcgcgc
    10861 gtttgcgtgg ttttgaactg ccggaagatg tggggcgttt cttgctgaag cggctcgaca
    10921 gagaaatgcg cacgctattt atgacgttgg atcagttgga tcgtgcgtcg attaccgcgc
    10981 aacgtaagct gaccattccg tttgtgaaag aaattctgaa gttgtagata aggtgtttat
    11041 tgtcggatgc ggcgcgaacg ccttatccga actacgcgaa gttaatccat agtattcgta
    11101 ggccggataa gacgtggcaa gcgtcgcatc cggcaatgtg cttcaactag cgatggagca
    11161 catcttaacc cacgatttcc agtacctgct ccggtggacg accaattcgc gctttgccat
    11221 tcgcgaccac aatcgggcgc tccatcagct tcggattatc caccattgcc tgaatcagcg
    11281 cctcttcgct aagcgaactg tcagccaggt tcagttcttt ataaagatcc tctttctggc
    11341 gcatcaattc acgggcgcta ttcatcccca gcattttcag caaatcgcgc agcgttgccg
    11401 catcggcggg tgtctcaagg tagagcacca cttctggctc cacgccgttt tctttcagca
    11461 ggttcagcgt ttcccggctc tttgaacagc gtgggttatg gtagattttt acctgtttgg
    11521 tcattgttct tcctttaatg cgaattacat cttggtataa ggcttaaagc gttcctgcag
    11581 ctggcgcaac tggtcgatgc gcgcatcgta acgcgcttgt tgcaggctgc ctaatttcac
    11641 ctgcgaactg gcgctactca acagcgaaat ggcctgatcg agtcgtccgg cgagcgcata
    11701 accttctgct cgcgcagcca gctcctgatc gcggttattt agcgcggctt ccgcctgtgc
    11761 cagcaaatcc cagccgttgc tgtcatcttt attattaaag gtgtagcgat tcagaatatt
    11821 ggccgcttct tgtggttgac cgccttgcag ataagcgttc gccaggttga gctgcaacac
    11881 aggattggtg cgtaaatcgc gggcattttt cagacgattg atcgcctcat tggctttgtt
    11941 ttgcccaaga tcgatatcag tagccagatc gagataccat gcgttgccag gttctgccgc
    12001 cagtaacggt tgcagcgttt tacgcgcctc gtcgtattta ttggcttcca ttgcctgtaa
    12061 agcacgacca tattgtgctg ctcgttgctg acgaacgttt cctttcgccc attcatccag
    12121 caaatcactg gtgagctggt tacgtccgga attatacatc cccagtgtgc gcgctttcgc
    12181 cagatagaaa tcttccgacg actgcaccac catcgggcgc atctgattag cacggttgcg
    12241 ggcatctgcc agacgacttt ccggcaacgg gtgagtcaat aaaatttccg gcggacgcga
    12301 ggagtaacgc gcctgatcga gtaatttttc gaggaaggtc ggcatcgcct gcggatcgaa
    12361 tcctgagcgt tgcagcacct gaataccaat gcggtccgct tcctgttcat tttgctgggt
    12421 gaaactgatc atcccctgac gcgttcccgc cagtgtaccg gtcagcgccg ccatccccgc
    12481 ctgcggactg gccatcgcca gtaaaataga acctaacgcg ccgacccagg tcagcggcgc
    12541 gctgcgctgc tgatcttcca tcgctcgcgc caggtgacgt tgggtgacgt gggagatttc
    12601 gtgcgccata actgaagcca gttgactttc gttatcggaa taacggaaca gggcagagtg
    12661 cagcaccacg ttgccgccaa agaaagcaaa ggcgttaatt tcgtcgttgt tgatcagaaa
    12721 aaaatgaaac ggtgtcttaa ccgaattggc atgcgaaacc agacgcatcc ccagcgaatt
    12781 aatatattgc gttaacagcg ggtcattaat taacggcgcg ctgccgcgta gctggcggac
    12841 ataatagtcg cccatctgca tttcctgacc aatggaaagc gtgcttcctg cggaggttcc
    12901 catatccggc aaggtgtctg cgctgtcggc aaacgccggg gctacctgac caatggtcat
    12961 agcagcaatg agggttgcaa ccaggttttt tttcaactgc ctgaacataa cctctatcct
    13021 gtatttctga agatagtcat ttgaccgata ctgtgaaata tcgttcccac cgcgccgcct
    13081 ttgagtgtaa cggcgaagat tagcccactc aaatgacttt tcagtttcct gaatccgtca
    13141 taaaaagcga agtctttttt gtgacatttc gatacaattg cggatcgcaa tcccgctctt
    13201 gaggttcagg gaaggtcttt atgctcgaaa tgttgatgca atggtatcgc cgccgtttta
    13261 gcgacccgga agcgattgcc ttgctggtta ttttagttgc cggatttggc attatctttt
    13321 tctttagtgg cctgcttgct ccgttgctgg tggctattgt gctggcctat ttgctggaat
    13381 ggccaaccgt gcgcctgcaa tctattggct gctcccgccg ctgggcgacg tcgattgtat
    13441 tggtggtttt tgtcggtata ttgctactga tggcgttcgt ggtactgcct atcgcctggc
    13501 aacagggcat ctacttaatc cgcgatatgc cggggatgct caataagctt tctgactttg
    13561 ccgccacgtt gccgcgccgc tatccggcgt taatggatgc gggcattatt gatgcaatgg
    13621 ccgaaaatat gcgcagtcgg atgctgacca tgggcgattc ggtggtgaaa atttccctcg
    13681 cctcgctggt cggtttgctg accatagccg tctatctggt gctggtgcca ttgatggtct
    13741 tcttcctgct gaaagacaaa gagcagatgc tgaacgccgt tcgtcgggtg ctgccgcgta
    13801 accgtgggct ggcaggacag gtgtggaagg agatgaatca acaaatcacc aactatatcc
    13861 gcggcaaagt gctggagatg atcgtggtgg ggatcgccac ctggctgggg ttcttgctct
    13921 tcgggctgaa ctattcgctg ctgctggcag tgctggtcgg cttctcggtt cttattccgt
    13981 acattggcgc atttgtggtg accattccgg tgataggcgt ggcgctattc cagtttggtg
    14041 ctggcacgga attctggagc tgcttcgcgg tgtatctgat tattcaggcg cttgacggca
    14101 acctgttagt accggtgttg ttctccgaag cggttaacct gcatccgctg gtgattattt
    14161 tatcggtggt gatcttcggt ggtttgtggg gattctgggg cgtattcttc gccattccat
    14221 tggcgacgct gatcaaagcc gtgatccacg cctggcccga tgggcaaata gcgcaagaat
    14281 aatcttcagc ccgacgaact gtttcgccgg gctgattttt caggattcct gacgtaaata
    14341 aatagcccga taacacatac tcaccagcac tgcaccgccg ataatattac ccagcatcac
    14401 cgggagcaga ttggcggtaa taaaatggct taccgtcagt gccggaaaat tgtctgcgct
    14461 actgtgcgcc agttgccaga aggaggtagg ggcgaaatgg cgaatggcaa tggcgaacgg
    14521 aatcacaaac aaattggcga tacagtgctc aaagccactg gcgacaaaca gggtgatggg
    14581 caaaatcatg gcgacgattt tgtcgcataa cgaacgcccg caataactca tccacagcgc
    14641 caggcaaacc atcagattgc acataatgcc gaggctgaca gattcagtaa atgtatgatg
    14701 cattttgccc tcggcgcagt gtaaaaccgc gactccccac atcgcgtttt cactcatcac
    14761 cagcccggaa aaccagatta acaaactgaa acaggcaata cctgccagat taccgcaggc
    14821 caccagaagt gcgttaatca gccaagttcg ccaactaata acgccccgac tttttgccat
    14881 caccgtcatt accgacgagg tgaacaggct ggtgccgcaa accgccagca aaataaagcc
    14941 gagtgtaaag caaaggccgc ccaccagatg ggttaatgcc tgtgaagacg gggcatcggc
    15001 gataacggaa aggtaaaaag taaatccgat cgccataaac acgccagcgg acatcgccat
    15061 taaaaagacc gacactttac tgcgggcaat tttatgggcg gcaatccgtt cagcgattgc
    15121 cgcttttctg gcgctcaact gcaagtcgaa agagagtttg tttctcatgc atactcctgc
    15181 aaaagcagat tacaacacct cgcgaaccga gatccccaga cgctgcattc gtgacagcag
    15241 cgtggtgcgc ttcatcccta atcgtgtcgc cgcgccacgg ggcccggcaa caatgccatt
    15301 ggtttctcgc aatacctgaa caatgcgctg acgttcttct tcgtcattct ctggcgtcgt
    15361 cgggtgcagc aactgcgcca ttgaactgcg aagcgctgaa tcttcattta gcgtcggcag
    15421 taaacggctt tgtcggacat taagatgtaa attcagactg ttaccacgag tcaacagtac
    15481 cgcccgctca atcacgtttt ccagctcgcg cacgttgccc ggccaatccc acgacatcaa
    15541 ctggcgtagc gcctcggtcg ggatggcgtc aattgcgcga ttcatatggc gcgccatttt
    15601 ttgcgtgaaa tgttttgcta aaagagggat atcttccgga cggtcgcgta gcggcggcaa
    15661 ttccagtggg aagacattca ggcgataaaa gagatcgctg cgaaactggc gatcttcaac
    15721 catttgccac aaatcacggt tggtggcggc aatgactctg acattcaccg ggatcgttct
    15781 actcccgccg agacgctcaa tctcccgctc ctgcaatacg cgcagcagtt taggctgaag
    15841 ttctaacggc agatcgccaa tttcatcgag aaacaacgtg ccgccatcgg caatttcaaa
    15901 acgaccacga tgggtattaa tcgcaccagt aaacgcccct ttgtcatgac cgaataactc
    15961 actttccaga agactggcgg ggatggcagc gcagttgatt ttgaccagcg gcttgtcgcg
    16021 tcgcgggcta agttgatgga tcgctctggc gattacctct ttgccagttc cggtttcacc
    16081 gcaaatcaac accgtactgt cgctcttcgc cacaatatct acctgctgga gcaggtcttc
    16141 cattgcctgg ctttgataga taatgtcacc gacgcccaga ttcgaaagga gctgctcgct
    16201 aagctggtgg ttttcttgct gcaaactttc ctgcaaatcg gtcatgctac gccaggcatc
    16261 ggcattgcca acggcaatag cgatgcgatc ggctatgtgt tgtagtagct ggcagttttc
    16321 ctcactaaag agagtggaag aggtatgcgc cagcaacaat gcacccggtg tatggttgcc
    16381 aaaggtaagc ggtataagga gcgcagattc gcagccgtta gatgcaagta agtgtaataa
    16441 cgggtcgcgt tgccagagaa acagatcatc tgcacggtgc gtcaaggtcg gttgtcgggt
    16501 ttgtagcgtt tgtatcagca atacactgtc gccaggcata ttgcgtggca gacacgcaca
    16561 atgtgaggca gaaagatcgc tgcaccacag gctgaacttc tcgttctttc gatgatcgcc
    16621 cagtaccata ctgactgaag ccagaccgaa aaaatgatgg atctcacggg cgacgtcagc
    16681 gatcagatcg tcgagatcaa gatgtgacag cactgcatta gtaatatcaa ccagtacccg
    16741 atgatgatcg caatctttct tcagcaaacg cagcgccacc tgctgttctt catcattgag
    16801 ccatgattgc agaaccgggg tgataatgcg tgccagccag ttaaacagtg tgcaatcctg
    16861 gggagtaaaa cgactgccgt gggtgcgaat aaagcgtagc tccgccattc cactactgcc
    16921 gagaaggacg gcgtgacgcg aataatcgac catctcgccc tgatactcta ctgttgccgt
    16981 cacatcgctg ccatcatcat ggtagcggta atagaagttg cttttgagaa catgcagttc
    17041 aataagttgt accgggctga acggcggcgg cagcgggatg aaggcgcgta ataaagacgc
    17101 cttgccgtgt ttctgcgcta accgctccgc gagcattccg tttaccgctt caattgttat
    17161 tccttgcggc ggggcaaaca tcgcctcgtc tgacatagcc atcaacagtc cttattaatg
    17221 agaaatcctt ctcacgcaat ttttctggcc atcagataga tggccggttc ctgcacaatt
    17281 tcatccaaca gatgaatgaa ttgaatgcta cgcgcctgcc acacagacgt cgcgtcatcc
    17341 gccagtggcg cgaatgcccg ggtcaacagg gcggtgtggc tggtgtcaat aacaatctca
    17401 ccaaaagtca tcacccccag catcttacgt cgggcttgct cctcttcgac cagtgcaagc
    17461 caatcttcgt attccgtcag tgggcagcag aaggcgacat tcagacaatc aatcacgcca
    17521 acgtgatgac cgatagccag tgagtaatac attacctgag agctgtgttc cggcatctcg
    17581 tcgctactgg cgacaaactt ttttcgcagc gtccagaaaa caatttcccc gcactcctca
    17641 gccatgtaac ccccggagaa cgcgaaagag ttgctgaacg atactattga gacgcggatc
    17701 gtcggcatcg cgcaaccagg tgttaacccg atttcctgta ggatcatcgc ttaaatgacg
    17761 caggagccga tcgcaaattt ctcgcccctg acgatagccc gtcagccgcc gcgcttctcg
    17821 ctcaatggcg atacgctgtg atggcgggat ctgcggccac aacggttgca tagtcacccc
    17881 ggtgggatcg cgataatcca cagcgtgaat cttctgttgc agcaaaccga gcgccacggc
    17941 gaaaccgtga atggtggcgg ccggtgttgg cgggcagccg gggatccaaa catcaatggg
    18001 gacaatggtg tcgctgccgc cccagacgct gtagagatcg tggaaaatac cgccgccgac
    18061 accgcacgcg ccgtaggaaa cacaaatttt attatcgggg gcagactcat acgcccgaag
    18121 tgcaggcata cgcatcgccc gggtgactgc gccagtaaat aacaaaatat cggcgtgacg
    18181 cggtgatgaa acaaccttaa tgccaaaacg ttctgcgtcg aatacgggtg tgatggcagc
    18241 aaaaatttcg atttcacagg cgttgcagcc gccgcaatcg acgcgataaa cgtaagccga
    18301 gcgacggata tcttgtaaca aatgctgctt catcttttgc gtttgctcgt ccagcgtgat
    18361 gggctggctg acatgttgcg taagcactgg actcatagct gctccttagc caccagcggt
    18421 acatctgtat cgtcgttgat cagcgtcgcg cgttgtttgc actccgggca gacgctcgct
    18481 tgcgcccgca acatttcgcg gttttgtggt gcattttgtt gctgtgccaa caattcagca
    18541 gccagtgcga cggttttttg cggggcaaac gggcgttcgc aacggctgca acgttgtaga
    18601 tggaacgtcg cgcgggtata gagatcggct ttattggtga cggtcagttc aaagttattg
    18661 gtaagctgga tggctctggt cgggcacact tcttcacaac gtccgcagta aatacaacgc
    18721 cccagataga gctgccaggt gcgcgaattt tgctggtcgt cggtctggat agtcagcgca
    18781 tttgccggac aagcacaggc gcaggcaccg caggcaatac attggctggg catcaggtcc
    18841 ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga agggatattt caccgtcgcg
    18901 gttccggcgc gcataatagt tttcagtaac ttcagcatct tctgtcctta cttcagcggc
    18961 gaacggttac gatcaatgcc gtagcgttcg atctctttat acggcacggt ttttgactgg
    19021 cgcttgcgca catcaaccag cgtcacacgg tcggtacagg agtagcaggg atcaaggcta
    19081 ccgataatca gcggtgcgtc agaaacggta ttgccgcgta gcatgtaacg cagcaccggc
    19141 cagttggcgt aggtggcggc acgacagcgc cagcggaaca atttttggtt atcaccgagc
    19201 atgctccagt gcacgtcttc accgcgtggc gcttcaacaa agccgagcgc gaatgcgtga
    19261 ggtttatagc taaagccttc ggtcagcagc ggggtatccg gcatgttgtc gagggcaaat
    19321 tccagcattg ccagcgaatc aaacgtctct ttgacacgga ccatcacgcg ggagaaaaca
    19381 tcgccgccgg tgaaggtaaa cagtgttttt ggaatattgc cgtagtcggc gtaggggtga
    19441 tcaaagcgca aatcacgggc aaaaccactg ccgcggatca gcggccctac agggctataa
    19501 tcacgggcga tttgtcggtc gagaatgcca atgccctgag tgcgttgttc catattcggc
    19561 gtagcaagca gcatctctac cagctccgac acgtcggcgc gcatctcgcg caccagtttc
    19621 agcgtttgca gacgttgctc tttgagaata tcgcggcgaa caccaccaat cagattcaga
    19681 ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg tcatcgactt ttcccgcacg
    19741 cgaaaaaatt gcataaagcc ggtatcaaaa ccaacaaaat ggcaggagag gccaaggttg
    19801 agcaaatgac tgtgtagccg ttcgacttcc agcagaatcg agcgaatggt atgcgctcgt
    19861 tgcggcacct caatcctcag tgcattttca accgagttgg tataggccac actgtgggca
    19921 aaaccgcaaa tcccacacac gcggtccgat aagaaggtca cttcgttgta gcccatccgc
    19981 gtttctgcca gtttctccat gccgcgatgg acataaaaca gacggtaatc agcatcgaca
    20041 atttgctcgc catccacaaa caagcggaag tgacccggtt catcggaggt gatatgcagc
    20101 gggccaatag ggatcacccg cgcgtcgctg tcgccctcgt tgataaacgg atacgtttcg
    20161 gtatcagtcg tcggttcagg gcgcagtcga taatccatcg cgtctttgcg cagcggatgc
    20221 atatcttccg gccagtcatc gggcaacacc aggcgacgct gatccggcag gccaaccgga
    20281 atcagcccgt acatatcgcg aatttctcgc tcgccccaga ccgcggcagg gacgcgcggt
    20341 gtgactgacg gaaactcccg actgtcggca tcgaccagtg ccttcaccac gatccagcat
    20401 ttttcggccc cttccataga aagggcataa taaacggcgt aatgaccgtt aagtgtccgc
    20461 tcgtcgttgc caaacaggac cggaagccag ccatcatgtt gataataaag atactgtaca
    20521 acgtcaggca gcagattgat tttcaccgta atggtgacct gttcaagcgt ttgtcgctct
    20581 tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg cgagaatcgc ttcgccacga
    20641 tttgacgatg aattaacgtt cactgtgttt ctccgaaaca gatgcggtgc ctgaaggcaa
    20701 aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc gtccctgaga ggactatagt
    20761 ggaagcgcct gccaggatcc tgatgacagg ttgtggaata tgcgttccca tcgccagcat
    20821 catgaccagc agaatcacca ttggcgaggt ggtcaaccag ccaagttcac cccggttaac
    20881 ggcctgcggc ggtttcgcca ttaacacccg cgcagccatc cgtaccaggc ccgccagcac
    20941 cagcgttaac agcaataaca gcaggacgat aatcagcaga tgattacgtg ccagtccggc
    21001 ggtaacggtc ataaattcgc taagaaaaat gttgaagggc ggcatccctg ccagcgccag
    21061 cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt ttgagcatcc cacagacgac
    21121 gttgagatcg cgcgtgccgt acttgagcag tacattgccg gaaccgcaga acagcagcgt
    21181 ttttgccaga ctgtggttta aggtgtgcag cagcgcggca aaaattccca gcggcccgcc
    21241 aatgcctagc gccaccgcga ccagtcccat gttctccacg ctggagtacg ccagcagacg
    21301 cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg gcaaccgaca acatgccgaa
    21361 gatgagcaac aaccggttgg ggaaatcgct gccgatggcc tggcaaataa tgatgtaata
    21421 gcgaatcagc accaacagcg cgcagttcag caatacggcg gagagtaggg cgctgaccgg
    21481 actcggcgct tcactgtgag catccggcag ccaggcgtgc atgggaaata gcccggtttt
    21541 ggtaccaaag ccaattagca caaacacaaa ggccaacagc attaatgttg gatcaagcaa
    21601 ggacgattgc ttaagaacct cgctccagaa tatcgccatt tctgcctgcg gcataacgct
    21661 ggcggcgttg gcgtatacca gcacggtacc gaacagacca aaagcgacac caacagtaca
    21721 aataatgatg tacttccatg cagcttccag cgatgaacgc tgaccgtaaa tgcctaccag
    21781 aaacgccgag cttaaggtgg tggcttcgat cgccgcccac atcacaatca ggttattgct
    21841 ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag aaaccgtagt aatcgcacag
    21901 cgtaacgggt gaaagctcgc cgtgctccac ttcatgacgc atgtagccaa tcgagtaaac
    21961 accggtaaga aagccaatca caccaagaat ggcgaggaac aaaccgccca gaccatcaat
    22021 atgaagccac agtcccgcag cgaatatttc tcccgcatca gcggcggttt ggaccaccca
    22081 gagtgccaga atcagcagta gtgtgatccc taagctatgt aatacggtca ccgcgcgagt
    22141 cgcagagtgc ccccgtttcc ggcaggcaaa acagagcagc gaaaaaagca gcggcgtgag
    22201 caggagtaaa gcgaacatca cagaataact catctcatta tcccttcagc gcggtcaagt
    22261 tgttcacgtc cagcgtgccg tgggtacgcc atatttttct tgccagtaac accatcacaa
    22321 tgacggcgaa gatggcgtcg gtagcgatac ctatttccac cagttccggt gcacgccagg
    22381 caagaagcgc cagcaccaga tgggagccgt tttccatcag gcagtaacca aaaatttgcc
    22441 gcaggatatt gcgctggctg acaatgcaca gcaggccaag cagaaaatga cctaacgcta
    22501 ccgccagcgc cggtttcagc ccggtagcca tcggtagctt cacgggctga acgacaaatg
    22561 cgcaaagcag gacaattaac gccgcgagca gtgccatcat tgccggaccg aataacgctt
    22621 tttccgggat gttctgggga atatttcgtg cagcgtaagt catgattaac ggtaccagca
    22681 gcactttggt gataaaggcg ctggcggacc agatcagcag ttgctctgcg gcgaacaggc
    22741 acgagagagt ggcgaaaata gacaccagca ccagcgactg gcaggcgtaa aatccgcatg
    22801 acaggcgata gcttttgacg ctaatcacaa acagcgatgt cagcatcatc agtcccgcca
    22861 gattatttac gatcatagaa ccggtcattc atcatctcct tactgcaacc aggtcgcggc
    22921 gattacgctg gaaatcagcg acatcacaat cagtacaatc aacaccaggc gcattgatcc
    22981 tggcagcggt gcggcatcgg cgacggcctc gctcggtttg ccaggcacga cgcgaccaaa
    23041 ccagcgaata aaccaagcga aactggcgac cgattcaatc atcagcagaa tcatggcggg
    23101 cagcaggatc cagtactcca ctgacaacgc aaaaccggcg gcaaacagcg ggaatttact
    23161 gaagaagccg ttgaacggcg gcacgccggt aatcgccagc gctgccacgc agaaacccac
    23221 gcctggcaac ggcagggtgt gcaatacgcc acgcagacgc ggcaacaagc gcgtgccgca
    23281 gctgtaactc agcgcacctg ctacaaggaa aaacaggctt ttagcgaacg cgtggttgac
    23341 gatgtaggcg atgctaccct ccagcgccag ccgcgagccg aagatggaga gcgacaagcc
    23401 gaagaacatc cagccaagtt gagtgatggt cgaccaggcc agcaaccgct tcatatcctg
    23461 ctgtggcaaa tacatcagaa atccataaag aatggtgacc agggccatga ccatgccaac
    23521 accgccaatc acatgcggga tattgccgcc gtcgataatg gcgcgggcaa aaatgtaaac
    23581 gcccactttc accatcgatg cggcgtggag ataggcgctg atcggtgttg gcgcttccat
    23641 tgcgtccggt agccacgctt gcatcggtag ctgggccgat ttcccccacg cggcaaacag
    23701 gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac tcgctcatcg cgctaagcgc
    23761 aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca acgagccgat
    23821 atgggtgata agcaacgctt ttagtgctga acgctgcgct ttatcgctct ggtaataact
    23881 gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac aacaactgac cgagcagcgt
    23941 cgaggagagt accagtcccg ccatcgcgcc gataaacaac agcaaaaatg cgtaataacg
    24001 attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc agataacccg tggagtagat
    24061 cgtgaccagc aaaccgagaa acaccaccac aaacagaatc agcgtactca cgcgatcaat
    24121 gaccaggcca aacaatgcca catcccccac gttgaccaac gtcaacgtga cggcaacttt
    24181 atcggcctga taaaacgcgg agatcagtga caacatgcac agcgtggtca gcgcggcgaa
    24241 caaaaccccc cattcggcgg cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat
    24301 aaaaggcagc aataacgtcg tcagagcaag attttccata ttccgtcagt gctccttaca
    24361 gaccggttaa ccagaagacc catgcaagcg cagcaaggct gaagccaagc caggtcacat
    24421 ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgatagag gccagaacaa
    24481 aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag gcgagagaaa
    24541 gctcttgcgc gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc atcacgacct
    24601 gtttcagccc cagcccccat ttcgctagcg ccagcccggc accggaatat tcggtcagcg
    24661 ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt cccatctcaa
    24721 tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc gagttccagc
    24781 ccgttgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc agcgccaata
    24841 ccagcagtga gagaatgagc atgggttcga ccagaatgcc gagcgtcaac tcgcgactgg
    24901 caccgactcc cgcaaacggg cttccggtat ccagcccgga aagagcaaag aaaaaacgaa
    24961 acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa ggggaaacgg
    25021 taataaatag tggtagggcc atcgccagca ccagcatgct gctgattaat acccacggca
    25081 tcaggcggaa catcagacct gaagatgtcg gcgcaacttc ctggcgttta aacagtttgt
    25141 ggatatcgcg ataatcctgc cagatccccg gtccgcggcg ggagtgcatt cgcgcgcgta
    25201 tctgccggga aatacccgta aaaagtgggg ttagcatcag cagaatcacg gcctgtgcta
    25261 acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct catggtgatt tccttagacg
    25321 gcaatagcga ttagcagcac aaccagtgcg gcgaccagat acaggcaata gagacgaaag
    25381 tcgccgcttt gcagatgctg tatttctttg gccagccgtt gggtggcgct cacgatgggg
    25441 cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt gacgtgcgca
    25501 agacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa tagcgcagaa
    25561 aagaccacac gcagcggctg catcacgcca ttgcctgacg gggccatcgc attttcccag
    25621 ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg cgaacgacac
    25681 cacagccagt aaaggccagg cagcaaaggc atcgccagta gtaacagcaa cagtaatgag
    25741 ggggtgacct gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc gacagtgacg
    25801 ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag ccaactggca
    25861 ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat catcgtattt
    25921 ggcacttcct gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa agtaataccg
    25981 tacattttta cgaagcacat tactgccagc ccaccagtga ctgccagcat taccatagca
    26041 ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa cagcgactgc
    26101 caggtgtacc attcgctgat aaaaccattc agcggaggaa tggctgatat cgcgaggcaa
    26161 ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc cattttttcc
    26221 atgtcgtggg tatgcaaacg cgaaataatc gcgcccgcgc cgagaaatag tagccctttg
    26281 aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaaccac ggtgagcagc
    26341 gggtcgtgca gtgacagacc gaccatcgcc acaccgactg ccagcaaaat aatgccgacg
    26401 ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag cgcatatagc
    26461 acgcccagga gcgcggagat tgtgccgatc gccatcacca gaatgcccca ccacagcggc
    26521 aaacccgttt gcgccagcag atccatcgcc actttcagga tgccgaaaat accgattttg
    26581 accattacgc cagacatcaa cgccgaagcg tgcgatggtg ctgcagggtg agcgcgcggc
    26641 aaccagctgt gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa ggccagcagg
    26701 aacaccgccg acgccagccc cggagaaagt gaaagcgtgc ggaaactggc aaaatcgagg
    26761 ctgccgcttt cgcgtcccat cagcaagaag gcgatcataa tcagcaccga tccggcgtgg
    26821 gcgataaaaa agtagagcat gccagcgttg atcgacgttt tatcctgcct ggcaatgacc
    26881 agaaaccagg aagacagtga catcatttca aacagcacga tgaaccaaaa agcgttgtcc
    26941 atcaccagca gggcaaccat cgatgcgatg aaaatattca taaagaagcc catcgccgcc
    27001 gcgcctttgc cctcgtattc gcgcatataa gtcaatgaat agagcgaaca aaccaccacc
    27061 agcaaggaga taaccagcac cataaatgcc gagaggctat ccattcgcag ggttaaatcg
    27121 gcaaagtcaa aaggggtggc aaaacgggcg actaaagtta cgcctgcatg taattgcgta
    27181 attccgctga taatgccaat cacgccgccc accagcgatg tgatgccgga aagcttaata
    27241 gccagtctgt ccagaccgag taaaaacagc gaagccagac tggcaaacag atagaggatc
    27301 agcgaccagg ttaataattg cagggcatcc attagcgttg ctcctgagtg agggaaaggg
    27361 gatcctcccc gccattggca aaccagcttg cggcaaggcg ctgtttttct ttcatctgac
    27421 gttgcaggct atcgtcggtg atcagccgta acgcctgatt cgggcaagcg cgaacgcagg
    27481 ccggaccttg tggcaagaaa tcacacaggt cgcatttcac cgcgacggtc tggacacccg
    27541 gttcccagcg cagcaaagca tgttgggttg gcgcgttttc ttcgccgtct ttgagtgagc
    27601 cttcagcctg aaaaacatat tgcgcagggg cattcaccgg acggcttcct gaagcgctga
    27661 ttgcgccaaa tgggcagacc acggcgcaaa gcttgcagcc aatacagagg ttttcgttga
    27721 gttggatcgc atcatccctc tgggagatgg cattgaccgg gcagacctgc aggcacgggg
    27781 cttcctcaca gtgatgacac acgacagggg cagtgagtgt tgacgtcttc gccagggcca
    27841 ggcgcgggtg ttgctgtaaa ccttgcgttt tatgcacgtc cgaacaggca gcgagacagg
    27901 tattacatcc tgtacaccac agtggttcgg ccaccacaaa gcggttcatg tcggctcctt
    27961 gaaaatgata tgaaacattg catttcatat tgcataaagg atgccagtct gattaattta
    28021 atatattcaa taagttatga attaactgga ttctgaacgg tacaaaaatg atggtcatta
    28081 acgttcagca aaaaatggat tcgacacccg tgtcgcaggg gaacgcgaga atgtgatctt
    28141 gatcacgaga agtgaggtat gctgaccgca agcgcagcca gcacggaatg gagcaaagta
    28201 atcaggcgtg ttctttcaac cagttcagca caacgtcgtg gtgattgctg gttttgaaat
    28261 cgtcaaagac atgttcgatt ttgccatcag cgtcaatcag gaagctgatg cgatgaatac
    28321 catcgtaggt tttgcccatg aaggactttt ccccccagac gtcgaattgc tcgcacacct
    28381 ggtggtcctc atcagacagg agcgtaaagt taagcagctc tttttccgca aaacgggaga
    28441 gtttttcggg tttatcggtg ctgataccca gcacatcaac gcccgctttt ttcaactcat
    28501 ccatgttatc gcgtaagccg caggcctgta cggtacagcc gggggtcatg gctttcgggt
    28561 agaaataaac cagaacacgc tgtccctgga agtcggtcaa attaacttgt tctccgtctt
    28621 gatccggcaa gctaaatttc ggtgcgatat caccggcttt cagtggattc attacttaac
    28681 tccatcctgt tcatcatgtt gggaataatt gacgacgtta atactgcctt gtgcattgag
    28741 ttctgtacat agggctttga acgcttgctc aatatttgct gcgtccgcag atgcggggct
    28801 gtgggcagta atctgaatat gcaactgcgc agcccgttca ttttcagcag gttgcgtgcg
    28861 cgacaccagc tccgcaatgt tcatatgatg cgcgtcgaaa agtgctgtga agcgttcaat
    28921 taaatgcggg gagtctgcca catcgacctg aacccagaca gatgctggca ttggcggacg
    28981 cggacgcgcc gtcgtgcgct tcatcacgat taaaagatcc agttcggcac ctttcaacgg
    29041 taacgttgat tcaatcagag taatggcatt ccatgaaccg gaaagcagca tgataaacgt
    29101 gaactcttct cccagcatcg ccaggcgact gtcttcaata ttacagccgc aactactgac
    29161 atgacgggtg atggtgttca caattccagg gcgatcggca cccaacgcag tgatcaccag
    29221 ataatgttgc gatgacagtg tcaaactggt tattccttta aggggtgagt tgttcttaag
    29281 gaaagcataa aaaaaacatg catacaacaa tcagaacggt tctgtctgct tgcttttaat
    29341 gccataccaa acgtaccatt gagacacttg tttgcacaga ggatggccca tgttcacggg
    29401 aagtattgtc gcgattgtta ctccgatgga tgaaaaaggt aatgtctgtc gggctagctt
    29461 gaaaaaactg attgattatc atgtcgccag cggtacttcg gcgatcgttt ctgttggcac
    29521 cactggcgag tccgctacct taaatcatga cgaacatgct gatgtggtga tgatgacgct
    29581 ggagctggct gacgggcgca ttccggtgat tgccgggacc ggtgctaacg ctactgcgga
    29641 agccattagc ctgacgcagc gcttcaatga cagtggtatc gtcggctgcc tgacggtaac
    29701 cccttactac aatcgtccgt cgcaagaagg tttgtatcag catttcaaag ccatcgctga
    29761 gcatactgac ctgccgcaaa ttctgtataa tgtgccgtcc cgtactggct gcgatctgct
    29821 cccggaaacg gtgggccgtc tggcgaaagt aaaaaatatt atcggaatca aagaggcaac
    29881 agggaactta acgcgtgtaa accagatcaa agagctggtt tcagatgatt ttgttctgct
    29941 gagcggcgat gatgcgagcg cgctggactt catgcaatta ggcggtcatg gggttatttc
    30001 cgttacggct aacgtcgcag cgcgtgatat ggcccagatg tgcaaactgg cagcagaagg
    30061 gcattttgcc gaggcacgcg ttattaatca gcgtctgatg ccattacaca acaaactatt
    30121 tgtcgaaccc aatccaatcc cggtgaaatg ggcatgtaag gaactgggtc ttgtggcgac
    30181 cgatacgctg cgcctgccaa tgacaccaat caccgacagt ggtcgtgaga cggtcagagc
    30241 ggcgcttaag catgccggtt tgctgtaaag tttagggaga tttgatggct tactctgttc
    30301 aaaagtcgcg cctggcaaag gttgcgggtg tttcgcttgt tttattactc gctgcctgta
    30361 gttctgactc acgctataag cgtcaggtca gtggtgatga agcctacctg gaagcggcac
    30421 cgcttgcgga gcttcatgcc ccggctggaa tgattttgcc ggtgacctcc ggtgattatg
    30481 caatcccggt gaccaacggt agtggtgctg tcggtaaggc gctggacatt cgtccaccag
    30541 cccagccgct ggcactagtt tctggcgcgc gtacccagtt cacgggcgat accgcttcat
    30601 tgctggtgga aaatggtcgt ggcaatactc tgtggccgca ggtggttagc gtgctgcagg
    30661 cgaaaaacta caccatcacc caacgtgatg atgctggtca gacactgacc accgattggg
    30721 tacaatggaa ccgtctggac gaagacgagc agtatcgtgg tcgttatcaa atctctgtta
    30781 agccgcaggg ttatcagcag gcggttacgg ttaaactgct gaacctggaa caggcgggca
    30841 aaccggttgc agacgcggct tccatgcagc gttacagcac ggagatgatg aacgttattt
    30901 ccgccggtct ggataaatct gccactgacg ccgcgaacgc tgcgcaaaat cgtgcctcca
    30961 ccactatgga cgtacaaagt gcagctgatg acaccggttt accaatgctg gtcgtacgcg
    31021 ggccgttcaa tgtggtttgg caacgtctgc cagcggcgct ggaaaaagtg ggcatgaaag
    31081 tgaccgacag cacccgttcg cagggcaaca tggccgtaac ttataagccg ctgtctgaca
    31141 gcgactggca ggaactgggc gcgagcgatc caggcctggc atccggtgac tataaactgc
    31201 aggttggcga tttagataac cgcagcagcc tacagttcat cgatccgaaa ggtcatactc
    31261 tgactcagag tcagaacgac gcgctggtag ctgtcttcca ggctgcgttt agcaagtaaa
    31321 aatacagggc tggaatcatc cggccctttt ttctgatatg atacgcaaac gtgtgcgtcg
    31381 gcaggaaaac gcgattttag cggtaattcg cacgaaattt gtttgtcgga cgtagttcgg
    31441 ataaggcgtt cacgccgcat ccgacaaaac atccggcaca ccagacagca aaagatttta
    31501 aaacgttaat tcacacccag gagtgataaa gatgcaaaag caagctgagt tgtatcgtgg
    31561 taaagcgaaa accgtataca gcacggaaaa cccggacctg ttggtgctcg aattccgcaa
    31621 tgatacgtca gcaggggatg gcgcgcgcat tgagcagttt gatcgcaaag gtatggtgaa
    31681 caacaagttc aactacttca ttatgagcaa actggctgaa gcgggtatcc cgactcaaat
    31741 ggagcgtctg ctctccgata ccgaatgtct ggtgaaaaag ctggatatgg taccggttga
    31801 gtgtgtcgtg cgtaaccgtg ctgcgggctc tctggtgaaa cgtcttggaa tcgaagaagg
    31861 tattgagctg aacccgccgc tgttcgatct gtttctgaaa aacgacgcca tgcacgatcc
    31921 gatggtcaac gaatcttact gcgaaacctt tggctgggta agcaaagaga acctggcgcg
    31981 tatgaaagag ctgacctaca aagcgaacga cgtgctgaaa aaactgttcg atgatgctgg
    32041 tctgattctg gtcgacttca agctggaatt tggtctgtac aaaggcgaag tggtactggg
    32101 tgatgagttc tccccggacg gtagccgcct gtgggacaaa gaaacgctgg agaaaatgga
    32161 caaagaccgt ttccgccaga gcctcggtgg cctgatcgag gcctatgaag ccgtcgcccg
    32221 ccgcctgggt gtacagctgg actgattttt ctgttcatca tcttgccgtg ctgctggcac
    32281 ggcaagacaa ccattctcgt aagatgtgca ttgaacgctc attcctccgc catttcatcc
    32341 cgctttcctg tggtaatctt cctctgaacc acctacgatc atttctataa tgaatatatt
    32401 gttgaggtat ctatgcgttg gcaagggcga cgtgaaagtg acaatgttga agacaggcgc
    32461 aacagctctg gtggtccgtc tatgggcggt cccggttttc gcctgccgag cggtaaaggc
    32521 gggctgattt tactgatagt cgtgctggtt gcaggctact atggcgttga tttaaccggg
    32581 ttgatgaccg gacagccggt ttcccaacag caatcaacgc ggtcaattag cccaaatgaa
    32641 gacgaagccg caaaattcac ctcggtgatt ctggcaacta cggaagacac ctggggacaa
    32701 cagttcgaga agatgggtaa gacctatcag caaccgaagc tggtcatgta ccgtggaatg
    32761 acgcgtaccg gatgcggggc gggccagtcc ataatggggc cgttctattg cccggcggat
    32821 ggcacggttt atatcgatct ctccttctat gatgacatga aagacaaact tggcgcggat
    32881 ggcgattttg cccaggggta cgttatcgcc catgaagtcg gtcatcatgt gcagaaactg
    32941 ttaggcatcg agccgaaagt tcgtcaactg caacaaaacg cgacgcaggc ggaagtgaac
    33001 cgcttatctg tgcgtatgga actccaggcc gactgttttg ccggtgtctg ggggcatagt
    33061 atgcagcagc aaggcgttct ggaaaccggc gatctggaag aggcgctgaa cgcggcgcag
    33121 gccatcggcg atgaccgttt acaacagcaa agtcaggggc gagtagtacc agacagtttc
    33181 actcatggca cttctcagca acgctacagc tggtttaaac gtggtttcga cagcggcgat
    33241 ccggcacaat gcaatacttt tggtaaaagc atttaacttc cggggcaggg atggctgaac
    33301 tgactgcgct tcacacatta acagcgcaaa tgaaacgtga agggatccgc cgcttgctgg
    33361 tgttgagcgg ggaagagggt tggtgttttg atcatgcgct taagttacgt gatgccttac
    33421 ctggcgactg gctgtggatt tcgccgcagc cagatgctga aaaccactgt tctccctcgg
    33481 cactacaaac tttacttggg cgcgagttcc ggcatgcggt attcgacgcc cgctacggct
    33541 ttgatgccgc tgcctttgcg gcacttagcg gaacgttgaa agcgggaagc tggctggttt
    33601 tgttactccc tgtatgggaa gagtgggaaa accaacctga tgccgactcg ctgcgctgga
    33661 gtgattgccc tgaccctatt gcgacgccgc attttgtcca gcatttcaaa cgcgtactta
    33721 cggcggataa cgacgctatc ctctggcggc aaaaccagcc gttctcgttg gcgcatttta
    33781 ctccccgtac tgactggcac cccgcgaccg gcgcaccaca accagaacaa cagcaactct
    33841 tacagcagtt actgaccatg ccgtcgggcg tggcagcggt aactgctgcg cgtgggcgcg
    33901 gtaagtcggc gttggcaggg caactcattt ctcgtattgc gggcagtgcg attgtcaccg
    33961 cgcccgcaaa agcggcaacg gatgtactgg cacaatttgc gggcgagaag tttcgcttta
    34021 ttgcgccgga tgccttgtta gccagcgatg agcaagccga ctggctggtg gtcgacgaag
    34081 ccgcagccat acctgcgccg ttgttgcatc aactggtatc gcgttttcct cgaacgttgt
    34141 taaccactac ggtgcagggc tacgaaggca ccggacgtgg ttttttgctg aaattttgcg
    34201 ctcgctttcc gcatttacac cgttttgaac tgcaacagcc gatccgctgg gcacagggat
    34261 gcccgctgga aaaaatggtt agtaatgcac tggtttttga cgatgaaaac ttcacccata
    34321 caccacaagg caatattgtc atttccgcat ttgaacagac gttatggcga agtgaaccag
    34381 aaacgccgtt aaaggtttat cagttattgt ctggtgcgca ctaccggact tcgccgctgg
    34441 atttacgccg catgatggat gcaccagggc aacatttttt acaggcggct ggcgaaaacg
    34501 agattgccgg ggcgctgtgg ctggtggatg aggggggatt atctcaacaa ctcagtcagg
    34561 cggtatgggc aggttttcgt cgcccgcggg gtaatctggt ggcccagtcg ctggcggcgc
    34621 acggcagcaa tccactggcg gcgacattgc gcggacggcg ggtcagccgg atagcagttc
    34681 atccggctcg tcagcgggaa ggcacaggac agcaacttat tgctggtgct ttgcaatata
    34741 cgcaagacct cgactatctt tcggtgagtt ttggttacac cggggagtta tggcgtttct
    34801 ggcaacgctg cggttttgtg ctggtgcgaa tgggtaatca tcgtgaagcc agcagcggtt
    34861 gctatacggc gatggcgctg ttaccgatga gtgatgcggg taaacagctg gctgaacgtg
    34921 agcattaccg tttacgtcgc gatgcgcaag ctctcgcgca gtggaatggc gaaatgcttc
    34981 ctgttgatcc actaaacgat atcgtgcttt ctgacgacga ctggcttgaa ctggccggtt
    35041 ttgctttcgc tcatcgtccg ctattaacat cgttaggttg cttattgcgt ctgctacaaa
    35101 ccagtgaact ggcattaccg gcgctgcgtg ggcgtttaca gaaaaacgcc agtgacgcgc
    35161 agttatgtac cacacttaaa ctttcaggcc gcaagatgtt actggtccgt cagcgggaag
    35221 aggccgcgca ggcactgttc gcacttaatg atgttcgcac tgagcgtctg cgcgatcgca
    35281 taacgcaatg gcaatttttt cactgactcc ttcaattttc tcccatggtc attgtgagtc
    35341 agcggcgtaa aattaatgac atcagttaag gagttcgcca tgaaacatga ccattttgtt
    35401 gttcaaagcc cggataaacc tgcgcaacag ttgttactgc ttttccacgg tgtcggggat
    35461 aacccggtgg caatggggga aataggcagc tggtttgcgc cgctatttcc tgatgcgctg
    35521 gtggtgagtg tcggaggcgc ggagccgagc ggtaatccgg cggggcgtca gtggttttcg
    35581 gtgcagggta ttacggaaga taatcgccag gcgcgtgtgg atgcgatcat gccgacgttt
    35641 attgaaacgg tacgctactg gcagaaacag agtggagtag gggctaacgc cacggcgcta
    35701 attggtttct cacaaggcgc gattatggcg ctggagagca ttaaagccga accgggcctt
    35761 gcctcacgcg tcattgcctt taacggacgc tatgcgagcc tgccggaaac cgcgtctaca
    35821 gccacgacaa ttcacctgat tcatggcggt gaagatccgg ttatcgatct ggcgcatgca
    35881 gttgcggcgc aggaagcgtt aatcagtgcc ggtggtgacg tgacgctgga tatcgtggag
    35941 gatttaggcc atgcgatcga caatcgcagt atgcaatttg ccctggatca cttgcgttat
    36001 accattccga aacattattt cgacgaagcg ctaagcggcg gtaagcctgg cgatgatgat
    36061 gtgattgaaa tgatgtaata atagccgggt aggcctgata agaccgtatc agacatcatg
    36121 gccggatgcg gcgttaacgc cttatccggc cactcagctt acttcttcgg ttgatccttt
    36181 ttcggccagt cgtcgtcatc gtcccactta tcgttgaaat cacgatgcgg gggaagctcc
    36241 ggcttgttgg cgagaaattt cttatggtca acgcgcttga gatctttaat cacgttcagc
    36301 aggacgccta ccaaaaaaac aatcaccaga atccaccaat atttagccag ccagtccatg
    36361 cttatttcct cttgcagaac cactcatcac gcgacgagct gttccatgat acgttgatac
    36421 atacgggcaa gtagctgcag gtcggcagcg ttcacacatt cattaatttt atgaatagtg
    36481 gcattgaccg gcccgagttc caccacctgc gcccccatgc gggcaataaa gcgcccgtcg
    36541 gacgttccgc ctgtggtcag tagctgcggt ttaatttcat tatagtgctc aaccgcgtta
    36601 acgaccgcat ccaccagttt accgcgcgcg gtcaaaaatg gctgcccgga aagccaccaa
    36661 tccaccgtat agcgcagttg atgtttttca agcagggcaa gcacctgcgc tttgatcatc
    36721 tcatcagtca gttcggtgct gaagcggaag ttaaactgca caaacagttc acccggaata
    36781 acgttgttac tgcccgttcc cgcctgaata ttggcaatct gcatactggt cgccgggaaa
    36841 aattcattgc cctgatccca ctcaatagcc actaattcat taaggaaagg tgctgcgcga
    36901 tgtaccggat tgtcagccag atgtgggtag gcaacatgcc cctgaacgcc atgaatggta
    36961 aggttgcagg ttaacgatcc gcgacgacca tttttcacca catcacctac cacttcgata
    37021 ctcgacggtt cgccaaccag gcagtaatcg agacgctcat tacgtgccat taacgcttcg
    37081 acgactttta ccgtaccgtt gtgggcactg gcttcttcat cagaggtgat cagaaatgcc
    37141 agtcgccccg tatggttggg atgttgtgcg acaaaacgtt ctgcagctac caccatcgcc
    37201 gccagcgagc ctttcatatc tgccgcaccg cgcccgaata acatgccgtc acgaatggtt
    37261 ggttcaaacg gcggattgat ccaacgatcg gcgtcgccag gcggcaccac gtcggtatgc
    37321 ccggcaaagg ctaacgtttc accttgccca cgccatgccc aaaaattctg cgtatcggca
    37381 aagtccatgc gttcaacggt aaaaccgatc gcctgcaaac gttcaatcaa caaagcctgg
    37441 catcctgcgt catcaggact cagggaaggg cggcgaataa gctgttgtgt cagctcaata
    37501 accgggcacg acatagacta cacctcatgg aaaaattgct gataactgga atcactgaaa
    37561 cccagcagca taggcttacc aggcgcgcag agcaatggac gtttgataat tgcaggcatt
    37621 tcagtcatta atgccgccgc agaggccgca tcggtgattt tattgcgggt ggtttcgtcc
    37681 agtttacgcc aggttgtacc acgggtgttg agtaacgctt cccagcctaa ttcgttgata
    37741 aaaccgttca ataattcgct gtccagccca tcgacgcggt aatcatgaaa acgatagtcg
    37801 atgttattgg cttccagcca acggcgagcc tttttaatgg tgtcacaatt tttgatgccg
    37861 taaagtgtaa ccataatgaa tccttttt
//
LOCUS       DAZKFU020000012        36915 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZKFU020000012 DAZKFU020000000
VERSION     DAZKFU020000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 36915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.012"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(70..861)
                     /gene="kdgR"
                     /locus_tag="ACHOQQ_RS02495"
                     /old_locus_tag="pgaptmp_000499"
     CDS             complement(70..861)
                     /gene="kdgR"
                     /locus_tag="ACHOQQ_RS02495"
                     /old_locus_tag="pgaptmp_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGU4468804.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            1038..2411
                     /gene="yebQ"
                     /locus_tag="ACHOQQ_RS02500"
                     /old_locus_tag="pgaptmp_000500"
     CDS             1038..2411
                     /gene="yebQ"
                     /locus_tag="ACHOQQ_RS02500"
                     /old_locus_tag="pgaptmp_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4468805.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMADLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMYALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(2461..3342)
                     /gene="htpX"
                     /locus_tag="ACHOQQ_RS02505"
                     /old_locus_tag="pgaptmp_000501"
     CDS             complement(2461..3342)
                     /gene="htpX"
                     /locus_tag="ACHOQQ_RS02505"
                     /old_locus_tag="pgaptmp_000501"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGU4468806.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(3534..5582)
                     /gene="prc"
                     /locus_tag="ACHOQQ_RS02510"
                     /old_locus_tag="pgaptmp_000502"
     CDS             complement(3534..5582)
                     /gene="prc"
                     /locus_tag="ACHOQQ_RS02510"
                     /old_locus_tag="pgaptmp_000502"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGU4468807.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(5602..6300)
                     /gene="proQ"
                     /locus_tag="ACHOQQ_RS02515"
                     /old_locus_tag="pgaptmp_000503"
     CDS             complement(5602..6300)
                     /gene="proQ"
                     /locus_tag="ACHOQQ_RS02515"
                     /old_locus_tag="pgaptmp_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGU4468808.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(6397..6894)
                     /gene="msrC"
                     /locus_tag="ACHOQQ_RS02520"
                     /old_locus_tag="pgaptmp_000504"
     CDS             complement(6397..6894)
                     /gene="msrC"
                     /locus_tag="ACHOQQ_RS02520"
                     /old_locus_tag="pgaptmp_000504"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGU4468809.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7024..8307
                     /gene="yebS"
                     /locus_tag="ACHOQQ_RS02525"
                     /old_locus_tag="pgaptmp_000505"
     CDS             7024..8307
                     /gene="yebS"
                     /locus_tag="ACHOQQ_RS02525"
                     /old_locus_tag="pgaptmp_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGU4468810.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            8276..10909
                     /gene="yebT"
                     /locus_tag="ACHOQQ_RS02530"
                     /old_locus_tag="pgaptmp_000506"
     CDS             8276..10909
                     /gene="yebT"
                     /locus_tag="ACHOQQ_RS02530"
                     /old_locus_tag="pgaptmp_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGU4468811.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            10989..12428
                     /gene="rsmF"
                     /locus_tag="ACHOQQ_RS02535"
                     /old_locus_tag="pgaptmp_000507"
     CDS             10989..12428
                     /gene="rsmF"
                     /locus_tag="ACHOQQ_RS02535"
                     /old_locus_tag="pgaptmp_000507"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGU4468812.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRPWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            12546..12782
                     /gene="yebV"
                     /locus_tag="ACHOQQ_RS02540"
                     /old_locus_tag="pgaptmp_000508"
     CDS             12546..12782
                     /gene="yebV"
                     /locus_tag="ACHOQQ_RS02540"
                     /old_locus_tag="pgaptmp_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGU4468813.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            12887..13078
                     /gene="yebW"
                     /locus_tag="ACHOQQ_RS02545"
                     /old_locus_tag="pgaptmp_000509"
     CDS             12887..13078
                     /gene="yebW"
                     /locus_tag="ACHOQQ_RS02545"
                     /old_locus_tag="pgaptmp_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGU4468814.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(13079..13735)
                     /gene="pphA"
                     /locus_tag="ACHOQQ_RS02550"
                     /old_locus_tag="pgaptmp_000510"
     CDS             complement(13079..13735)
                     /gene="pphA"
                     /locus_tag="ACHOQQ_RS02550"
                     /old_locus_tag="pgaptmp_000510"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4468815.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(14131..14472)
                     /gene="yebY"
                     /locus_tag="ACHOQQ_RS02555"
                     /old_locus_tag="pgaptmp_000511"
     CDS             complement(14131..14472)
                     /gene="yebY"
                     /locus_tag="ACHOQQ_RS02555"
                     /old_locus_tag="pgaptmp_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGU4468816.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(14485..15357)
                     /gene="copD"
                     /locus_tag="ACHOQQ_RS02560"
                     /old_locus_tag="pgaptmp_000512"
     CDS             complement(14485..15357)
                     /gene="copD"
                     /locus_tag="ACHOQQ_RS02560"
                     /old_locus_tag="pgaptmp_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGU4468817.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(15361..15735)
                     /gene="yobA"
                     /locus_tag="ACHOQQ_RS02565"
                     /old_locus_tag="pgaptmp_000513"
     CDS             complement(15361..15735)
                     /gene="yobA"
                     /locus_tag="ACHOQQ_RS02565"
                     /old_locus_tag="pgaptmp_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGU4468818.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWRVVSVDGHKTKGHYTFSVK"
     gene            15874..16104
                     /gene="holE"
                     /locus_tag="ACHOQQ_RS02570"
                     /old_locus_tag="pgaptmp_000514"
     CDS             15874..16104
                     /gene="holE"
                     /locus_tag="ACHOQQ_RS02570"
                     /old_locus_tag="pgaptmp_000514"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGU4468819.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            16206..16862
                     /gene="yobB"
                     /locus_tag="ACHOQQ_RS02575"
                     /old_locus_tag="pgaptmp_000515"
     CDS             16206..16862
                     /gene="yobB"
                     /locus_tag="ACHOQQ_RS02575"
                     /old_locus_tag="pgaptmp_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGU4468820.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            16886..17548
                     /gene="exoX"
                     /locus_tag="ACHOQQ_RS02580"
                     /old_locus_tag="pgaptmp_000516"
     CDS             16886..17548
                     /gene="exoX"
                     /locus_tag="ACHOQQ_RS02580"
                     /old_locus_tag="pgaptmp_000516"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGU4468821.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(17545..19605)
                     /gene="ptrB"
                     /locus_tag="ACHOQQ_RS02585"
                     /old_locus_tag="pgaptmp_000517"
     CDS             complement(17545..19605)
                     /gene="ptrB"
                     /locus_tag="ACHOQQ_RS02585"
                     /old_locus_tag="pgaptmp_000517"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGU4468822.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRNGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(19814..20473)
                     /gene="yebE"
                     /locus_tag="ACHOQQ_RS02590"
                     /old_locus_tag="pgaptmp_000518"
     CDS             complement(19814..20473)
                     /gene="yebE"
                     /locus_tag="ACHOQQ_RS02590"
                     /old_locus_tag="pgaptmp_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4468823.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(20799..21155)
                     /gene="yebF"
                     /locus_tag="ACHOQQ_RS02595"
                     /old_locus_tag="pgaptmp_000519"
     CDS             complement(20799..21155)
                     /gene="yebF"
                     /locus_tag="ACHOQQ_RS02595"
                     /old_locus_tag="pgaptmp_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGU4468824.1"
                     /translation="MKKRGAFLGLLLVSVCASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVVWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(21222..21512)
                     /gene="yebG"
                     /locus_tag="ACHOQQ_RS02600"
                     /old_locus_tag="pgaptmp_000520"
     CDS             complement(21222..21512)
                     /gene="yebG"
                     /locus_tag="ACHOQQ_RS02600"
                     /old_locus_tag="pgaptmp_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGU4468825.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            21646..22824
                     /gene="purT"
                     /locus_tag="ACHOQQ_RS02605"
                     /old_locus_tag="pgaptmp_000521"
     CDS             21646..22824
                     /gene="purT"
                     /locus_tag="ACHOQQ_RS02605"
                     /old_locus_tag="pgaptmp_000521"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGU4468826.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(22880..23521)
                     /gene="kdgA"
                     /locus_tag="ACHOQQ_RS02610"
                     /old_locus_tag="pgaptmp_000522"
                     /gene_synonym="eda"
     CDS             complement(22880..23521)
                     /gene="kdgA"
                     /locus_tag="ACHOQQ_RS02610"
                     /old_locus_tag="pgaptmp_000522"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU4468827.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(23558..25369)
                     /gene="edd"
                     /locus_tag="ACHOQQ_RS02615"
                     /old_locus_tag="pgaptmp_000523"
     CDS             complement(23558..25369)
                     /gene="edd"
                     /locus_tag="ACHOQQ_RS02615"
                     /old_locus_tag="pgaptmp_000523"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGU4468828.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(25604..27079)
                     /gene="zwf"
                     /locus_tag="ACHOQQ_RS02620"
                     /old_locus_tag="pgaptmp_000524"
     CDS             complement(25604..27079)
                     /gene="zwf"
                     /locus_tag="ACHOQQ_RS02620"
                     /old_locus_tag="pgaptmp_000524"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGU4468829.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            27417..28286
                     /gene="hexR"
                     /locus_tag="ACHOQQ_RS02625"
                     /old_locus_tag="pgaptmp_000525"
                     /gene_synonym="yebK"
     CDS             27417..28286
                     /gene="hexR"
                     /locus_tag="ACHOQQ_RS02625"
                     /old_locus_tag="pgaptmp_000525"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGU4468830.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            28414..29856
                     /gene="pyk"
                     /locus_tag="ACHOQQ_RS02630"
                     /old_locus_tag="pgaptmp_000526"
     CDS             28414..29856
                     /gene="pyk"
                     /locus_tag="ACHOQQ_RS02630"
                     /old_locus_tag="pgaptmp_000526"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGU4468831.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(29987..30958)
                     /gene="lpxM"
                     /locus_tag="ACHOQQ_RS02635"
                     /old_locus_tag="pgaptmp_000527"
                     /gene_synonym="msbB"
     CDS             complement(29987..30958)
                     /gene="lpxM"
                     /locus_tag="ACHOQQ_RS02635"
                     /old_locus_tag="pgaptmp_000527"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU4468832.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLMIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(31078..32400)
                     /gene="mepM"
                     /locus_tag="ACHOQQ_RS02640"
                     /old_locus_tag="pgaptmp_000528"
     CDS             complement(31078..32400)
                     /gene="mepM"
                     /locus_tag="ACHOQQ_RS02640"
                     /old_locus_tag="pgaptmp_000528"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGU4468833.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(32416..33348)
                     /gene="znuA"
                     /locus_tag="ACHOQQ_RS02645"
                     /old_locus_tag="pgaptmp_000529"
     CDS             complement(32416..33348)
                     /gene="znuA"
                     /locus_tag="ACHOQQ_RS02645"
                     /old_locus_tag="pgaptmp_000529"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGU4468834.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            33427..34182
                     /gene="znuC"
                     /locus_tag="ACHOQQ_RS02650"
                     /old_locus_tag="pgaptmp_000530"
     CDS             33427..34182
                     /gene="znuC"
                     /locus_tag="ACHOQQ_RS02650"
                     /old_locus_tag="pgaptmp_000530"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGU4468835.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            34179..34964
                     /gene="znuB"
                     /locus_tag="ACHOQQ_RS02655"
                     /old_locus_tag="pgaptmp_000531"
     CDS             34179..34964
                     /gene="znuB"
                     /locus_tag="ACHOQQ_RS02655"
                     /old_locus_tag="pgaptmp_000531"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGU4468836.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(35178..36188)
                     /gene="ruvB"
                     /locus_tag="ACHOQQ_RS02660"
                     /old_locus_tag="pgaptmp_000532"
     CDS             complement(35178..36188)
                     /gene="ruvB"
                     /locus_tag="ACHOQQ_RS02660"
                     /old_locus_tag="pgaptmp_000532"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGU4468837.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(36197..36808)
                     /gene="ruvA"
                     /locus_tag="ACHOQQ_RS02665"
                     /old_locus_tag="pgaptmp_000533"
     CDS             complement(36197..36808)
                     /gene="ruvA"
                     /locus_tag="ACHOQQ_RS02665"
                     /old_locus_tag="pgaptmp_000533"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGU4468838.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         8757 a         9579 c         9153 g         9426 t
ORIGIN      
        1 tcaactttat ttttctcatg ctaaataaag aacctgtctg tttgatattc cgacaggttc
       61 ttactctcat cagaacggat agtcgtgata acccatttgg gcagaaattt tgcgcgctgc
      121 ggtatgcaac attgcgacat attcctgtaa acgctcttca gagaaacgca acgtcgggaa
      181 ggagatgctc aaaccggcaa tgaccacgcc aaagcgatca aataccggaa ccgcaatgca
      241 tcgcagccct tcttcctgct cttcgttatc ttcgccatac ccctgctcgc gcacctggtc
      301 cagaaccggt aataacgctt ctgtactggt gatggtccgc tcggtactgc gtttatactc
      361 cacgccctca agaatttgct tcacttcatc gcgatcgcgc catgccagca gtaccttacc
      421 aatcgcggtg ctgtacagtg gattacgacg cccaatccgt gaatacatgc gcaaattgta
      481 catagagtca attttgtgaa tgtaaacaat actgtcttcg tccagtgcgc cgaggtggat
      541 agtttctttg gtcaggcggg agatctcacg catctggata tctgcgctac gaattaaatc
      601 gacgttttgt aacgcgcgag cgcccagttc aaacaatttc agggtcaggg aatatttctc
      661 cgactcccct tcctgcgcca cataacctaa ggttttcatg gtctgtaaaa agcgataaac
      721 ggtgcttttt gacatcatga cgcgctgcga cagctcggtt atccctattt cgcgctcttc
      781 acccagcgcc tgcaaaatgc caaaaacttt tagcacggaa gatacagaat caggctgttt
      841 atccagatct gcgttagcca tttatcacct cattgcgagt gttttataaa aatcagaact
      901 gttttttatt ataatttcgc accagggtgg tcgcaatcca tcttttgccg gttagttaca
      961 attctgcgac atccaccgtg aatatcagtg ctagaatcat acccctgttg attattcacc
     1021 aaagatttaa aattcctatg ccaaaagttc aggccgacgg cctgccattg ccccagcgat
     1081 acggtgcgat attaaccatt gtgattggta tttcgatggc tgaccttgac ggcgcaatcg
     1141 ccaacgtcgc cctgccaaca atcgccacgg accttcatgc cacgccagcc agttccatct
     1201 gggtagtgaa cgcctatcaa atcgccattg tcatctccct gctctcgttt tcgtttctgg
     1261 gtgatatgtt tggctatcga cgtatttata aatgcggtct ggtcgttttt ctgttgtctt
     1321 cactgttctg cgccctttct gattcgctgc aaatactcac ccttgcgcgt gtcatacaag
     1381 gtttcggcgg tgcagcgttg atgagcgtta ataccgccct tatccgcctg atctatccac
     1441 aacgttttct gggtagaggg atgggcataa actcgtttat tgttgccgtc tcttctgctg
     1501 ccgggccgac aattgctgca gcaatcctct ccatcgcatc ctggaaatgg ttatttttaa
     1561 tcaacgtacc gttaggtatt atcgccctgc ttctggcgat gcgttttctg ccgcccaatg
     1621 gttctcgcgc cagtaaaccc cgtttcgacc tgcccagcgc cgtgatgtac gcgttaacct
     1681 tcggcctgct tatcactgcg ttgagtggtt tcgctcaggg gcaatcgctg acgttaattg
     1741 ctgcggaact ggtggtaatg gttgttgttg gtattttctt tattcgccgc cagctttctc
     1801 ttcccgtacc gctgctaccg gtggatttac tgcgtatccc gctgttttca ctttctattt
     1861 gcacatctgt ttgctctttc tgcgcacaaa tgctggcaat ggtttccctg cccttttacc
     1921 tgcaaaccgt cctcgggcgt agtgaagtcg aaacaggttt acttctgaca ccgtggccgt
     1981 tagcaacgat ggtgatggct ccgttggcag gctatttgat tgaacgcgta catgcaggat
     2041 tgctgggtgc tttaggatta ttcatcatgg ctgcggggct tttttccctg gttctgctgc
     2101 ccgcgtcacc tgcggatatc aatattatct ggccgatgat cttatgtggc gctggatttg
     2161 gcttgttcca gtcacccaat aaccacacca ttattacctc cgctcctcgc gaacgtagcg
     2221 gtggagccag tggcatgtta ggaacggctc gtctactggg tcagagtagc ggcgcggcgc
     2281 tggtggcgct gatgctaaat cagtttggag ataatggtac acacgtctcg ctgatggctg
     2341 cggctattct ggcagtgatt gctgcctgtg tcagtggttt acgtatcact cagccacgat
     2401 ccagggcata aaaaaaagcg cgtcgatcag gacgcgcttt ttagtggtta cttcataata
     2461 ttacttcagg tattcacccg tacgcagagc ttcaattcgt ttatccagcg gcgggtgagt
     2521 catgaacaac tcactgagcg atttcgactt accgttaatg cagaaagcca tcatgctggt
     2581 tgcttcttgc ggttcatagc tggttttcag gcgctgcagc gcggcaatca ttttctcgcg
     2641 accaaccagt tttgccgaac cggcatcagc atggaattca cgatgacgcg agaaccacat
     2701 ggtgataatg ctcgccagaa taccaaacac cagttccaga accgttgcaa ccgcaaagta
     2761 gatcagcggg ttgccgttgc tctcttcacc ttcatcacga tttccgccca taaaacccgc
     2821 ggcaagctgc gccaggatac gggaaataaa gataacgaag gtgttcacca cgccctgaat
     2881 cagcgtcatg gtgaccatat caccattggc gatgtggctg atttcgtgag caattaccgc
     2941 ctcggcttca tccgggctca tgttctgcag caaaccggtg ctgacagcaa ccagagaggc
     3001 atcacggcgc gcaccggttg caaaagcgtt gatgtccggc gcatggtaga tagccacttg
     3061 cggcatagcg atccccgcct gacgagcctg ggttgctaca gtattgacca gccaacgttc
     3121 cctttcgtta cgcggttgct cgatcacttc cccgccaaca gatcgtaatg ccatccattt
     3181 ggacatcaga agcgaaacga aggaaccacc aaaaccgaac agcaaggcca tgatcatcag
     3241 cccctgaacg ctgctcgact gtatccctgt caggctcagt accagcccga aaacgaccat
     3301 tacggccagg ttcgttagca ggaagagcgc gattcgcatc ataattttct ttttacctca
     3361 gtttaacaaa acgcaatatg cgatacccac atcgtatggg ttacgcgact attttcaagt
     3421 ctggatagtg cgtaagtcac cagaaagaca caactttaca ttttgtagca tctgatttac
     3481 ggcatcttgc cgctgttaaa aaatcaggca caatttcttg tgcctgattg atattacttg
     3541 acgggtgcgg gttgttccgc gggtctggct ttttcaagct tcgccagatc gagtgcgata
     3601 ttcaccgtct catccagata aggatccggc tcctggtaat ctttcggtag atcatccagt
     3661 ttcttcaact ccggtttacc ttcgcgttta aagcgttcgt tcaaacgcgc cagacgcgtc
     3721 gcatcatctt cattattctc tttctcacgc acagcgtaat tcagagaaac gatattgcgc
     3781 ttgtccttca tggcgttgaa gcgcgcgata tccttcatga tgttctggaa ctcaggatct
     3841 ttcgcgatac gcgcattatg ttccttcagc agctccggtt caaaggccgt taaatcgcct
     3901 gatttcacat aggtcgcggc atcaatgcta tcccacggca gcgcgttatc ttcgaatttc
     3961 tcacccgttt ccgtttcttc attacccgtc ggcatgatga tgtctggcgt tacgccttta
     4021 cgttgcgtac tgccgccgtt aacgcgatag aatttctgga tcgtgtactg cacagaaccc
     4081 agcgctggcc attcaggacg taacatctga tcgtaaatac ggttcaatga acggtattgc
     4141 tgaacggtgc ctttaccaaa cgtcggttca cccacaacca gcgcacgacc gtaatcctgc
     4201 attgccgcgg caaagatttc tgaagccgaa gcactgaagc ggtcaaccag taccaccagc
     4261 gggcctttat agaaaacctg tccgtcggta tcgctgtctt cacgaacttt accgttgtta
     4321 tcacggacct gaacaatagg acccgcagga ataaacagac ctgagagcga taccgcttcg
     4381 gtcagcgccc caccaccatt gctacgcagg tcgatgatca cactgctgac attctgtttt
     4441 tccagtttct gcagttgtac tttgacatcg tctgtcaaac ccacatagaa gcccggaata
     4501 tccagtacgc cgactttctc tttaccgacg gtcttcaccg acattttaac cgcgcggtct
     4561 tcgagacgaa tacgttcacg ggtcaatgtt acagtgcggg tcttggtccc tttaccagca
     4621 ggtaaaattt ccagacgaac tttactgccc ttcggccctt taattaaggc aaccacatca
     4681 tcaagacgcc agccaatcac gtcaaccatc ggcttgcctg tttgaccaac accgacaatt
     4741 ttgtcaccaa cgctgatagc tttactcttc gctgccggac cacctgccac catcgaattg
     4801 ataacggtgt agtcatcatc catttgcagc actgcgccaa taccttccag cgacaaactc
     4861 atttcagtgt tgaactgttc ggtattacgc ggggaaagat agttggtatg cgggtcgatt
     4921 tcacgcgcaa acgccgtcat tgccagcgag aaaacatctt cgctgttggt ttgcgccaga
     4981 cgacgaatgg caaatttgta gcgtcgagtc agggtttcac gaatttcttt atccgttttt
     5041 cctgccagct tcaggcttaa ctcgtcgaat ttgactttac tgtcccacag cgcgttcaac
     5101 tcagcctcgt ttttcggcca gggcgctttg ctgcggtcaa ggttataagt gtcgttgccg
     5161 gtgaaatcca tcggcttttc cagtaccgac aaagcgtact ggtaacgttc aaaacgacgc
     5221 ttttgcgcca gattgtagag atcgtagaaa acgtcgagtt tgcctgaacg cagttcatcg
     5281 cctaactcgg ttttcttttt cgcgaactgt tcaacatcgc ttgccagcag cacgttgtgg
     5341 ctgtaatcga gcagattcag gtagcggtca aagattttgg ccgaaaatgc ctgatcgagg
     5401 tcgaactggc gataatgaga acgggtgaag cgcgacgtta cgcgctcact caccgtcgca
     5461 tgctgcgtct cttcctttaa taccggaatt tgatcagcac gcgtgatatc ttctacagcg
     5521 aaggtctggc ctgctattgc aagcaggcca gctaacgcgg taagcctaaa aaacatgttc
     5581 atgcctggcc cggcctccgt ttcagaacac caggtgttct gcgcgcacaa tcaaagacat
     5641 acccgaattc agctggacgc ggacgccgtc tttggtgatt tctaatacgg tggcatccat
     5701 cgcgttttga cccgctttca ccttcagggc ttgtccgaca gtcagagctg aaatgtcaga
     5761 aactggggtg tgctgttctt cgcgaggtgc ttttgctgtt tttggcgctt tctctaccgg
     5821 cttttgcgca cgaggtttac gttcagcgcc ttctttgcgg cgtggcgtgg tcggacgtgg
     5881 cttgcgttcg cgacgcggtg cgtcttcttt ctcaccagca gctgcggcag cttcgcgttt
     5941 tttcgcttgc tgttcagcac gctgtgcctg aacacgcgct ttcgcttctt caagctgctt
     6001 gcgagcatgc tctacatgtt gctcgtccag ctcaccgcat gggttgccgt caagatcgac
     6061 acgcgttgcg ccgggtttaa caccgtaaag gtaacgccag ctcgaagtgt agagacgtaa
     6121 agcggatcgt aattgcgttt tgctcaggtt catttccccg gcaacacgat cgaccaaatc
     6181 ctgaaaaata ccgattttca gcggacgcgc ttcaccttcc gcactgaaac agtggggaaa
     6241 acgttcggcc agaaacgcga ttacttcttt actgctattc aacttaggtt gattttccat
     6301 gaaatttcct gattacaacg gacgtagcca acaagcgcag gcatgaacag gcgtcattat
     6361 aatgacgcta tcagtaaatg ctacgttatc cgttgattat cctgcgacgc tcgcaaagaa
     6421 ttttttgtaa tccgtcgttg caagcacctt ttcaagctgt gccacaagct gacgtaagcc
     6481 ttgctcgtcc tcgtctgtaa agcgaccgaa gacggtacta tcgatgtcga gaacaccaat
     6541 aatctgattt ttcaccacca gcggcagaac aatttcagaa ttactcgccg catcacaggc
     6601 aatatgcccg tcaaacgcat gaacatcctc gatacgctgc acttgattgc gggcaaccgc
     6661 agtgccgcac acgccacgcc cgacgggtat ccggacacag gcaattttgc cctgaaatgg
     6721 tccgagcacc agtgtatcgt cctcaagcaa ataaaaacct gcccagttta cgtcagtgag
     6781 acgctcatat aacaacgcac tggtgttcgc aagcgttgcc agaaaactgg tttctcccgc
     6841 catcagcgcg ttaaagtcgc gatttaaatc cgcgtaaaat tctgttttgt tcattatata
     6901 atcacttggt tgtcttacct ggaactgcca gcctattaaa ataagcatta aatgcgttaa
     6961 tgctcaagat cattcccatc atgggttaag attaatgtta attcttatta catttggcac
     7021 gtcatggctc ttaacacacc acaaattacg ccgacaaaaa agataacagt gagggcaatc
     7081 ggcgaggaac tgccgcgtgg tgattaccaa cgttgcccgc aatgtgacat gctgtttagc
     7141 ctgcccgaga taaattctca tcaaagtgcc tattgtccgc gctgtcaggc aaaaattcgt
     7201 gacgggcgcg actggtcgct aacgcgcctg gcggcaatgg ccttcaccat gctgttgctg
     7261 atgccgtttg cctggggcga accgctgttg catatctggc tgttaggtat tcgtatcgac
     7321 gccaatgtca tgcaaggcat ctggcaaatg accaaacagg gcgatacgat aacgggggcg
     7381 atggtctttt tctgcgttat tggtgccccc ctcattctgg tgtcctccat agcatattta
     7441 tggtttggga accgactggg aatgaattta cgtccggtac tgctgatgct tgagcgactt
     7501 aaagagtggg taatgctcga tatctacctg gtcggcattg gcgttgcttc tataaaggta
     7561 caggattatg cccatatcca ggcgggtgtc ggcttgttct cttttgtggc gttggtgatt
     7621 ttaacgacgg tgacgttgtc acatcttaat gtcgaggagc tgtgggagcg attttatccg
     7681 cagcgccccg ctacgcgtag ggacgagaaa cttcgtgtct gtcttgggtg ccattttacc
     7741 ggctatcctg atcagcgtgg tcgctgcccg cgttgtcata ttccgctacg cctgcgtcgc
     7801 cgtcatagtt tgcaaaaatg ctgggcggcg ctgttagcgt caatcgtttt gttgttacct
     7861 gccaacctgt tgcctatttc tatcatttat ctgaatggtg gccggcagga agatacgatt
     7921 ctttccggga ttatgtcgct ggcaagtagc aacattgccg ttgcgggaat cgtgtttatc
     7981 gccagtattc tggtaccgtt tactaaagtg atcgtcatgt tcactttact gttgagcatt
     8041 cattttaaat gccagcaagg tttacgcaca cgcattctgt tactgcgaat ggtgacctgg
     8101 attggtcgct ggtcgatgct cgacctgttt gtcatatctt taaccatgtc gctgattaat
     8161 cgcgatcaga tcctcgcttt tactatggga ccggctgcgt tttatttcgg cgcagcggta
     8221 attttgacta ttcttgctgt ggaatggctg gacagccgct tactttggga tgcacatgag
     8281 tcaggaaacg cccgcttcga cgactgaagc gcagattaaa aataaacgcc gtatctcacc
     8341 tttctggctg ctgccgttca tcgcgctaat gattgccggt tggctgattt gggacagtta
     8401 tcaggaccgg ggtaataccg tcaccatcga ctttatgtcg gcggatggta ttgttccagg
     8461 ccgtacgcct gttcgttatc agggcgttga agtcggaaca gtgcaggata tcagcctcag
     8521 cgacgatctt cgtaagattg aagtcaaggt cagcatcaag tccgatatga aagatgcact
     8581 gcgcgaagag actcagttct ggctggtgac gccaaaagca tcgttggcag gtgtctccgg
     8641 gctggacgcc ctcgtcggtg ggaactatat cggcatgatg ccgggtaaag gtaaagagca
     8701 ggatcacttt gtcgcgctcg atacccaacc gaaatatcgg ctggacaatg gcgatctgat
     8761 gatccacctg caagcccccg atctcggttc gctgaacagc ggttcattgg tctatttccg
     8821 caagatcccg gtgggaaaag tctacgacta tgccatcaat cccaataaac aaggcgtggt
     8881 gattgatgtc ctgatcgagc ggcgttttac tgacctggtg aaaaaaggta gccgtttctg
     8941 gaatgtttct ggcgttgatg ccaacgtcag tatcagtggc gcgaaggtga aactggaaag
     9001 tctggcggca ctggttaacg gtgcgattgc cttcgattca cctgaagagt cgaaacctgc
     9061 cgaggcggaa gatacctttg gtctgtatga agatctggcc cacagccagc gtggcgtaat
     9121 aataaaactg gaactgccga gtggggccgg attaaccgcc gactcgacgc cattaatgta
     9181 tcagggactg gaagtcggac agctaactaa actggattta aatcctggtg gtaaagtcac
     9241 tggggaaatg accgttgatc ccagcgtcgt taccctgctt cgtgaaaata cccgcatcga
     9301 attacgtaat ccgaaattat cccttagcga tgccaatctc agcgccctgc tgactggcaa
     9361 aaccttcgag ttggtacccg gcgatggcga gccacgcaaa gagttcgttg ttgtgccagg
     9421 cgaaaaagca ctgctgcatg aacctgatgt tctgacgctg accctgaccg cgccggaaag
     9481 ttacggtatt gatgcgggtc agccgctcat tcttcacggc gtgcaggtag gccaggttat
     9541 cgatcgtaaa ctcaccagca aaggcgtcac ctttaccgtc gccatcgagc ctcagcatcg
     9601 agaactggta aaaggcgata gcaaatttgt cgtcaacagc cgtgtcgacg tgaaggtggg
     9661 gctggatggc gttgagtttc tcggtgccag cgcctcagaa tggattaacg gcgggatacg
     9721 tattctgccg ggcgataaag gcgagatgaa agccagctat ccactgtatg ccaatctgga
     9781 aaaagcgctg gagaacagcc ttagcgattt acccaccaca accgtgagtt tgagtgcaga
     9841 gacgctgccg gatgtgcagg caggatcggt agtgctgtac cgtaaatttg aagttggtga
     9901 agttattacc gtgcgtccgc gagctaacgc gtttgatatc gatctgcata ttaagccgga
     9961 gtatcgcaac cttctgacca gcaatagcgt gttctgggca gaaggcgggg cgaaagttca
    10021 gctgaatggt agtggcctga ccgtacaggc atccccgctc tccagagcat taaagggagc
    10081 cattagcttc gataacctca gcggtgccag cgccagtcag cgtaaaggcg ataagcgtat
    10141 tctgtatgct tccgaaacag cggcccgtgc ggttggcggg cagattacgc ttcacgcttt
    10201 cgatgccgga aaactggcgg tcgggatgcc aattcgctat ctcggtattg atatcgggca
    10261 aatccagaca ctggatctga ttaccgcgcg taatgaagta caggcaaagg cggtgctcta
    10321 tccggaatat gtccagactt ttgctcgcgg aggtacgcgc ttctcagtgg tcacaccgca
    10381 aatttcggca gctggcgttg agcatctaga tactatcctc cagccgtata tcaacgtcga
    10441 accaggccgt ggcaatcctc gtcgcgactt tgaattacaa gaggccacca ttactgattc
    10501 gcgttacctg gatggtttaa gcattattgt tgaagcgccg gaagccggtt cgttaggcat
    10561 cggtacgcct gtgctgttcc gtggtctgga agtaggtacg gttaccggaa tgacgctggg
    10621 gacattgtca gatcgcgtga tgattgcgat gcgcatcagt aaacgctatc aacacctggt
    10681 gcgtaacaat tccgtcttct ggttggcatc gggttacagt ctggattttg gtctgacggg
    10741 cggcgtagtg aaaaccggca cctttaacca gtttatccgt ggcggcatcg ccttcgccac
    10801 gcctccgggt acgccactgg caccgaaagc ccaggaaggc aagcacttcc tgttgcagga
    10861 aagtgaaccg aaagagtggc gtgaatgggg aactgcgctt cccaaataat gcccactgct
    10921 ccggcgtgcc tgcgccggag cgtttatgct aaactgcgcg cctgtttttt tgccagtggt
    10981 atatgctcgt ggcccaacac accgtttatt tcccggacgc ctttctgacg caaatgcgcg
    11041 aagcgatgcc ttcgacgctc tcttttgatg attttcttgc cgcctgtcag cgcccgttgc
    11101 gccgcagcat tcgcgttaat acgctgaaaa tctccgttgc tgatttcctg caattaaccg
    11161 ctccttatgg ctggacgctt acgccaattc cgtggtgtga agaaggtttc tggattgaac
    11221 gcgacaatga agatgcattg ccattgggta gtaccgccga gcatttaagc ggcctgtttt
    11281 atattcagga agccagttca atgttgcccg ttgccgcctt gtttgctgac gataatgcac
    11341 cacagcgggt gatggatgtc gcagctgcgc caggctccaa aacgacgcaa attgccgcgc
    11401 ggatgaataa cgaaggggca atccttgcca atgagttttc cgccagtcgg gtaaaagtgt
    11461 tacatgccaa tatcagccgc tgtggtatca gtaatgttgc gctcacacat tttgatggcc
    11521 gcgtgtttgg tgcggcattg ccagaaatgt tcgatgccat tttgctggac gctccctgct
    11581 ccggcgaagg cgtggtgcgt aaagatcccg atgcgctaaa aaactggtca ccagaaagca
    11641 atcaggaaat cgcagctacc caacgggaac tgatcgacag tgcctttcat gcattacgcc
    11701 ctggcggtac actggtttac tcgacctgta ccttaaaccg ggaagaaaac gaatccgttt
    11761 gcctgtggct taaagagact taccccgacg cagtagagtt tttaccactt ggcgatctct
    11821 tccctggtgc aaacaaagcg ctgaccgaag aaggcttttt gcatgttttc ccacaaattt
    11881 acgactgcga aggcttcttc gttgctcgtc tgcgtaaaac tcaggccatt cccgccttac
    11941 ccaccccaaa atacaaagtg ggcaatttcc cgttcagccc ggtgaaagat cgcgaagccg
    12001 cacaaattcg tcaggcggct gcaagtgttg gcttaaactg ggatggaaac ctgcgcccct
    12061 ggcagcgtga caaagaactg tggttgttcc cggtgggcat tgaagccctg atcggtaaag
    12121 tccgattttc tcgcttgggg attaaacttg ccgagacgca taacaaaggt tatcgctggc
    12181 agcatgaagc ggttattgcc cttgcctccc ccgacaacga gaacgctttt gaactgacac
    12241 cgcaagaagc ggaggagtgg tatcgcgggc gcgatgttta cccgcaagcc gcgccagtgg
    12301 cggatgacgt gttggttact ttccagcatc agccgattgg tttagccaaa cggattggtt
    12361 cgcgactgaa aaacagctac ccgcgtgaac tggtgcggga cgggaaactt tttaccagta
    12421 acgcatgaca gcgcacaaaa aaagcgcact ttttgactgg cacattcggc ggcctcaact
    12481 aggctgaaaa atggtgcgat cggactggtc gtaccacaat cggcagctaa atggagagca
    12541 cgaagatgaa aacgagtgtg cgcataggcg cttttgaaat cgacgacggc gaattacacg
    12601 gcgaatcgcc gggtgatcga acgttaacca ttccttgtaa atctgacccc gatttatgta
    12661 tgcaactgga tgcctgggat gctgaaacca gtatccctgc cctgctaaat ggcgaacact
    12721 ctgtccttta ccgtacccgt tacgatcaac aatctgatgc ctggattatg cgtcttgcct
    12781 gatccaaaaa gaacccgtcg gcatggcggg ttatttgtcc tggttattcc cccgttgtaa
    12841 aatctctcct aaacttaacg gtacggcacc acacttcggg gatgaaatgt tcgcgctggt
    12901 actttttgtt tgctacctgg atggcggttg tgaagatatt gttgtggatg tctacaacac
    12961 ggaacagcag tgtctttatt ctatgagcga tcaacggatc cgccatggcg gttgttttcc
    13021 gattgaggat tttatagatg gtttctggcg accagcacag gagtatggtg atttttaatt
    13081 attgcaattg cacgaaagtc agttcgcccc caaagacagc gccggtatca atataatgca
    13141 ggttgccaat atccacgcga tggcgcaacg gtgtatgacc aaaccagaaa tgatcagcac
    13201 ctgtaattcc ctgccctttt tggcgttcac ttaatcgcga gcggctccac aagacctgat
    13261 gcaaatcaac gtccttttgc cattcataaa catcatctgg ataatcggca tgagcaataa
    13321 catgtttgcc ggtgcggctg tgtacttcaa gaataaaggg caaatgctga catttttcca
    13381 gcgccgtttt cgcttgtttc tgttgattat ctgccagcgc aataaaccag tcgccgccat
    13441 tcatcaacca caaagacatc tgctgggatg ccagcgcatc catcgccatc tgttcatgat
    13501 tgcctcttac cgcacgaacc caatgttgtt ccagtaactg cagacaacgt aaactttgcg
    13561 gcccacgatc gataacgtct cccactgaga taagtaaatc tcgccacgga tcaaaacgac
    13621 aatgccataa tttgcggcgc aactgctcaa gacaaccgtg tatatcgcca gaaagccaga
    13681 tatgtcgcca ttgatgaccc gcaattctct gataaacggg cgcaggctgt ttcatcaata
    13741 ttttcctccc gcgctaaaga tcacataatc ttaacaagaa tgttaaaaaa cgctggactc
    13801 agacagtaga gtgtgtgtta tggttgacta taaagtcagc gaaggaaatg cttctggctt
    13861 ttaacagata aaaagagacc gaacacgatt cctgtattcg gtccagggaa atggctcttg
    13921 ggagagagcc gtgcgctaaa agttggcatt aatgcaggct tagttgcctt gccctttaag
    13981 aatagatgac gacgccaggt tttccagttt gcgtgcaaaa tggtcaataa aaagcgcggt
    14041 ggtcatcagc ttaaatgtta aaaaccgccc gttctggtga aagaactgag gcggtttttt
    14101 tattggaaat caaaaggcta ttttaggtaa ttaacagagt ttttcagccc gttctataaa
    14161 cggtgccaga ctcatttttt cgccgggatt gttgggatca tcaatctgaa tcaccgaaat
    14221 gggttgggct ttagtcttcc cactggcaac ttccttctgt gcgatatcgt ttaaaggata
    14281 ctgcacgagg gtacttgggt taatgacata caaagcatta cccggtctgc aagtcagcat
    14341 cacctcttcg cgattaaacg cccatttgtc tttacccact tcaaaacggc tgacggtaat
    14401 cacctgcggt gcagccagcg ccgctgcaga actggtgagt aacagaaacg ccagaatact
    14461 ttttttcatc attttcgtaa tccatcaaaa aggttcgaga gtcgcaagca ggctgatgat
    14521 caccagcact accgcaccta tcgcccattc gagtttagtc atccacacaa accagggtgc
    14581 cgcacgatct tcatcctgcc tcatgcgcgg tacaagaaca taacgattag ccagcgcaat
    14641 gaccaccata atcatcacca ggatggcttt gagcaataag agttggcccc agtaagtcgt
    14701 aagcgtgggg ggaaatccgg taatcaacaa tgcattaagc acgccgcttg ccagtacgcc
    14761 gatcaccgca aaatgcccgc accaggaaaa acgcatcagc gcctgaatag cctggtgtcg
    14821 ccaacggcct ttgatgagtt gcatacacca gagcaccggc agtaatcccc caaaccacgc
    14881 cgccgcacaa atcaggtgaa tcgcgtgatt agtctgatgg attttcgccg ttaccccttc
    14941 attcagcgtc gcatgcccca ctcccgccag cagaataaat tgcgcggtag tgagcataaa
    15001 cagcaaacgt ggcatattcc gcggttgcat aagggcgacg atcaacgtta cgagggcgag
    15061 aacaatttgc cataaccaga ccccaccaaa ctgcgtttgc aaaacagcct gccaaatgtt
    15121 tggcgaaaat acatctgtcc atcccgttcc catcaacccg ccctgaacgg cgagcattgc
    15181 agtggcactg atcaaactcc agacggcagc gtgctgttgt aaacgcagaa aacgcttcgt
    15241 cagcaagcga cgaatcgtta agggggccag ccaggcaccg tacattgcga agccaaaaac
    15301 cagcatcagc gaggtgaaat ggataaatcg cagcgcgatc caggtaaacg ccagcatgat
    15361 ttatttcacg ctaaaggtgt agtgtccttt cgttttatgc ccgtccaccg aaacaacacg
    15421 ccagtcaacg gtgtaggttc cgggtttcag cgaatcggcc agtggaacaa tcagttgctt
    15481 ttggtcctgc tcatttcgct tcgccggtaa tgttttaata ttttcgtttt ttggccccgt
    15541 gatttttgca ccactgaatc ctgtttcaac accttccgag aagtttaaag tgattgcctg
    15601 cggggcagct gtcacttgcg cgtttgccgc aggatactga tgcgttaaat gcgcatgtgc
    15661 ccagacggaa ggtgtcacca acgaagtggt caggattgcc agcgcgtagc gaagggagcg
    15721 tgcagttgaa gccatattat ctattccttt ttgtaataac ttttttacag agcataacct
    15781 tgtctaatgt ctgagtcgag gatcatcaat tccggcttgc catccaggct cactcttagt
    15841 aacttttgcc cgcgaatgat gaggagatta agaatgctga agaatctggc taaactggat
    15901 caaacagaaa tggataaagt gaatgtcgat ttggcggcgg ccggggtggc atttaaagaa
    15961 cgctacaata tgccggtgat cgctgaagcg gttgaacgtg aacagcctga acatttgcgc
    16021 agctggtttc gcgagcggct tattgcccac cgtttggctt cggtcaatct gtcacgttta
    16081 ccttacgagc ccaaacttaa ataaaactta tacagagtta cactttctta cataacgcct
    16141 gctaaattat gagtattttc taaaccgcac ccataatttg cagtcatttt gaaaaggaag
    16201 tcattatgtc gttctggaaa gttgcagcag cgcaatatga gcctcgtaaa acctcactca
    16261 ccgaacaggt agctcatcat ctggagtttg tgagggccgc cgccaggcaa caatgtcagc
    16321 tgttagtttt tccatcgctt tctttactgg ggtgtgatta ttcgcgacgt gcccttcctg
    16381 ccccacccga tctctcactg ttagacccgc tttgttatgc cgcaacgacc tggcgaatga
    16441 ccatcattgc cggccttccc gttgaatata acgatcgctt tattcgtgga attgcggtgt
    16501 tcgcaccgtg gcgaaaaacg cctgggatct accatcagag tcatggtgca tgtctgggcc
    16561 gtcgttccag aaccatcacg gtggtcgatg aacaaccgca aggtatggat atggacccga
    16621 cctgttcact gtttacgaca gggcaatgtc tgggagaacc cgacctgctg gcgtcggccc
    16681 gtcgcttaca atttttttca catcagtact cgattgccgt gctgatggca aatgcccgtg
    16741 gtaacagtgc actatgggat gaatatggtc gtctcatcgt tcgcgccgat cgtggttcat
    16801 tattgttagt cggtcagcgt tcatcacagg gttggcaagg cgatatcatt ccattacgct
    16861 aggctttttc ggcctggagc atgccatgtt gcgcattatc gatacagaaa cctgcggttt
    16921 gcagggaggg atcgttgaga ttgcctctgt tgatgtcatt gacggaaaaa tcgtcaaccc
    16981 catgagccac ctggtgcgcc ccgatcgtcc tattagtcca caagcgatgg cgattcatcg
    17041 catcaccgaa gccatggtcg ccgataaacc gtggattgaa gatgtgatcc cacactatta
    17101 cggtagtgaa tggtatgtcg cgcataacgc cagctttgac cgccgcgtac tgcctgagat
    17161 gcccggtgag tggatttgca ctatgaaact ggcccgtcgt ttgtggccag ggatcaagta
    17221 cagcaatatg gcgttatata aaacacggaa gctcaatgta cagacgccgc cgggcctgca
    17281 tcatcaccgc gcgttgtatg actgttatat caccgccgcg ttgcttatcg atattatgaa
    17341 cacctccggc tggacggcag aacagatggc cgatatcacc ggacgtccgt cgttgatgac
    17401 gaccttcacc tttggcaaat accgtggcaa agcggtttcc gacgttgccg aacgcgatcc
    17461 gggctatctg cgctggttat ttaataacct ggacagcatg agcccggagc tgcgtttaac
    17521 actgaaacat tatctggaaa atacttaatc cgcaggcgta gcgggtaatg ttccctgcgc
    17581 cagcgcgacc agaaaagcat attccatcgc tacgccttcg tacgatttaa agcgaccaga
    17641 tttaccgcca tggcctgagt ccatgtcggt acagagcaat aaaagatggt catcggtttt
    17701 cagctcgcgc aatttagcga cccattttgc cggttcccaa tattgcacct gagaatcgtg
    17761 caaaccggtc gttaccagta aatgcggata agcctgtgcg gtgacgttgt catatgggct
    17821 gtagcttttc atgtactcgt aatattgcgg atcctgcgga ttcccccact cttcaaactc
    17881 accagtggta agaggaattg attcatcaag catcgttgtt acaacatcaa caaacggtac
    17941 ctgggcgata acgccgtgga ataattccgg gcgttgatta attgcaacgc ccatcaacat
    18001 gccccccgca ctcccgccca tcgcataaca aagcgaagga gagccatagc ccagttttaa
    18061 caatgcatcg caggcatcaa gataatcatt aaacgtattt ttcttcttca gaaatttacc
    18121 gtcttcgtac cattgttgcc ccagctcacc gccgccgcga acatggacaa tggcgtagac
    18181 aaaaccacga tctaacaaac tcaagcggct aaaactgaaa tcggcatcaa tacttgcgcc
    18241 gtaagaacca tagccataca ccagcaacgg gttgtgccct ttgcgaaaat gtttgcgatg
    18301 gtagaccaac gaaaccggaa cttcgacgcc atcacgggcg actatccaca ggtgttcact
    18361 gcggtaattc gccgcatcaa aaccaggaac ttccgtttgt tttaatacac gacgctcacc
    18421 ggtatccata tccagttcaa acaaagtgtc tggtgtggtc atggaagaat aaccataacg
    18481 caatcgcgcg gtttcaggtt ctggattgta ggcaatccag gtcacatagg ccggatcatc
    18541 aaaggcaata ccaatgactt cccgggtctt gcggttaatt tggcgcaaac tggttaaccc
    18601 gcgctgacgc tcttcaacca ccagccagtc ggtaaacagc gtaaaccctt ccagcatgat
    18661 gttttcgcgt ggcggaatta actcttccca ctgttgctca tcacgcatac gggtacggta
    18721 taagccaaag tttttgccgt tgcggttgga acgcagataa aaacgatgct ggtagtgatc
    18781 aaggctgtat tcgtgatctt tgcggcgcgg cagaaaaaca aacggctcgg catcggccat
    18841 ttccgcgtcc agcaggcgaa cttcactggt ggtggcgctg gccaaatgaa tgactacata
    18901 gtgcttcgag gtcgttttat gcaggctgac gtaataggta tcgtcttttt cttcgtaaat
    18961 cagtttatct tgcgatgctg gcgtaccgat ggcgtgacgc cagacctgat aaggcagcag
    19021 cgtcaccgga tgcttgcgaa catagtagaa agtccaggag tcatttgccc agacaaagct
    19081 gggttcaacg ttatccagca gttccgggta ccagttacct gtttccagat tacgaaaacg
    19141 aatgccgtac tggcgtcggg aaagaaaatc ttctgccagc gccataatgg tgttatcggg
    19201 cgtaatcgcc attccgccca tcgaataaaa ctcactatga gccgcgcgct tgttggcatc
    19261 gagcaatatt tcccactcgt cccactcttc actgaacgcc gattgacgct ggtagatagc
    19321 atattcacag cctggttcat aaatatgccg atagcggtag ccatttttga tgtagggcgc
    19381 agaaacttct ctttgcggaa tgcggtcgat gatttccttt aagatgcgat cctgcaaggc
    19441 ttgttgtgag gccatcaccc gatgaccgta actattttct tgttgcaggt agtccaggac
    19501 ttccggctga gaacgcgtat cgtcccgcag ccagtagtaa ttatcgatgc gcgtatcgcc
    19561 atgaagcgtc atggcgtggg gaatgcgggc ggcttttggt agcatgttat tgttctttct
    19621 ggttgaaaca tcttataagg gtggcaaaac tcaccgggga tgcaagcgaa acagggcagt
    19681 cattgcttag atgatgacag gtaatggcgc gaatatcgaa tgttatgcag acagagaaaa
    19741 tcagcctgtt caaatggctg tgcggttctg gatagcccga aatagtcaac ttcaggctat
    19801 ccagagagcg ggattattcc gccaaagtgc gtttttgctg ttcgagatcg cgttcaatgc
    19861 cttcacgaac atcctggggg attttcagcg cgtcacccag tgcattcagg taactgcgtt
    19921 ccataaaatg gtcaatatca atagccgcgc agctcaggaa atagatttcc agcgcctctt
    19981 cttcattgcg gaccccggta gccaggcgtt gtggatccag cggttgttcg attgcctgct
    20041 caatgagtac ccgcccctgc tcttccacgc cggcttcacg caattgctgg tcgattgccg
    20101 cacgttcttt ggcatcaata tgaccatcac ttttagcggc aaagaccagc gcaaggatca
    20161 aacgttccgt acgctcatcg agcggcgtac tttgcgcgcc aaactgcggt tcgtcctgat
    20221 gcgccgcgcg aattttatct ttgtatttat tccacagcac cgtacccgct accgctccgc
    20281 cgccaaccag taacgcgttg gtgccatatt tcgtaagaag tttacgtgct gatttatttg
    20341 cgaccagcag cccagccagt ccgcctaatg cgcctggcac taacagtttg cccaatccct
    20401 gatccgcaga cgaggaggta gaagaactgc tttgcccaag cagggattgc agttgattta
    20461 accagttagc catatttgct cctcaataac catttttagt gcctgtagca tagcggatga
    20521 ggatgtcaga aaatgtatat cgagacaaaa gatgcgcaaa taagcggggc ggttggccgt
    20581 tattcaaaga aattcgcgcc aggtaaggtg tttactatat atcaggtgat aagggtattt
    20641 taggtgaaag tagtttgacg ttttgagtgg cactgtatga tgctgattgc ccgatgctac
    20701 gctgttgcgt cttctcaggc ctacgattgt ggtgtgttgt gggcggacaa ggcactcgcg
    20761 ccgcatccgc caacggtcaa tcgaattatc agaagccatt aacgccgctg atattccgcc
    20821 attcccgctt tgcagtccac gctgacctgg taatgaatat cggcactttt accacgtacg
    20881 gttagcggta ctgaccattt atcatcttta ccctgaatgt cctgcaaact gacccatacc
    20941 acgggatcgg cctgaccgac aattttttga tcatctgccc agcgcgccac gcgattttgt
    21001 tgataatcac gttttacgct cgcggcaatt ccggcagcat ccagaccttc acactttggg
    21061 aaagtgaccg acttgctggt ttcattattg gcagcgaaaa ctgatgcgca gacagaaacc
    21121 aacaacagcc ctaaaaacgc ccctcttttt ttcatgtttt tctccatagc acaatgattc
    21181 aggagaaagc atggtacaaa ttgtcaggag cgcaagttgc ttcaggccgc gtgtgaggca
    21241 tcttcctctt cggattcagc accgacgacc tgtggggacg gtaatttgcc ggttttcaga
    21301 atggtgctca gcacatcttt ttgttctgcc agccatagcg aaagggcttc acgttgctcg
    21361 tcttccattt gcactggaga atttgtcagc caggtgtcaa gcagatccgc cgtatcaagc
    21421 attttgtcat aagcatcggc ttcctttttg ctggtaaacg acattttctc ttcgccctca
    21481 cgaatgacta cgtatttaac ttcaaccgcc atttgcagcc tctcataata actgtgattt
    21541 tatacagtat atttcttttc ggttgagaaa tcaacaccag caataaagac acacgcaaac
    21601 gttttcgttt atactgcgcg cggaattaat caggggatat tcgttatgac gttattaggc
    21661 actgcgctgc gtccagcagc aactcgcgtg atgttattag gctccggtga actgggtaaa
    21721 gaagtggcaa tcgagtgtca gcgtctcggc gtagaggtga ttgccgtcga tcgctatgcc
    21781 gacgcaccag ccatgcatgt cgcgcatcgc tcccatgtca ttaatatgct tgatggtgat
    21841 gcattacgcc gtgtggttga actggaaaaa ccacattata tcgtgccgga gatcgaagct
    21901 attgccaccg atatgctgat ccaacttgaa gaggaaggac tgaatgttgt cccctgcgct
    21961 cgcgcaacga aattaacgat gaatcgcgag ggtatccgtc gcctggcggc agaagagctg
    22021 cagctgccca cttccactta tcgttttgcc gatagcgaaa gccttttccg agaggcggtt
    22081 gccgccattg gctatccctg cattgtaaaa ccggtgatga gctcttccgg caaggggcag
    22141 acgtttattc gctctgcaga gcaacttgct caggcatggg aatacgctca gcaaggcggt
    22201 cgcgccggag cgggccgcgt aattgttgaa ggcgtcgtta agtttgactt cgaaattacc
    22261 ctgctaaccg tcagcgcggt ggatggcgtc catttctgtg caccagtagg tcatcgccag
    22321 gaagatggcg actaccgtga atcctggcaa ccacagcaaa tgagcccgct tgcccttgaa
    22381 cgtgcgcagg agattgcccg taaagtggtg ctggcactgg gcggttatgg tctgtttggt
    22441 gtcgagctgt ttgtctgtgg tgatgaggtg attttcagtg aggtctcccc tcgtccacat
    22501 gataccggga tggtgacgtt aatttctcaa gatctctcag agtttgccct gcatgtacgt
    22561 gccttcctcg gacttccggt tggcgggatc cgtcagtatg gtcctgcagc ttctgccgtt
    22621 attctgccac aactgaccag tcagaatgtc acgtttgata atgtgcagaa tgccgtaggc
    22681 gcagatttgc agattcgttt atttggtaag ccggaaattg atggcagccg tcgtctgggg
    22741 gtggcactgg ctactgcaga gagtgttgtt gacgccattg aacgcgcgaa gcacgccgcc
    22801 ggacaggtaa aagtacaggg ttaaacccgg caaaaaaacg ctacaaaaat gcccgatcct
    22861 cgatcgggca ttttgacttt tacagcttag cgccttctac agcttcacgc gccagcttag
    22921 taatgcggtc gtaatcgccc gcttccagcg catctgccgg aaccagccag gaaccaccga
    22981 tgcacagcac gcttttcagc gccaggtagt cacggtagtt agccggagaa ataccacccg
    23041 tcgggcagaa acggacctgg gagaacggac ccgcgatcgc ctgcagggct ttcacgccgc
    23101 cgttagcttc agccgggaaa aatttgaact ctttcaaacc gtagtccata cccagcatca
    23161 gttcggaaac agtgctgatc cccggaatca gaggaatagt cccttcggta gcagctttca
    23221 gcagcggctc ggtcagaccc gggctaattg cgaactgtgc acccgcttcg gtgacttctg
    23281 ccagctgctg tggattcagc accgtaccgg cacccacaat cgcttcaggc acttctttgg
    23341 cgatagcacg gatagcgtca actgcacact cggtacgcag agtcacttcc agaacgcgca
    23401 ccccaccagc aaccaacgct tttgccatcg gcaccgcgtg ttccagtttt tttaccacga
    23461 taaccggtac aaccgggccg gtggtcagga ttgattctgc acttgttttc cagtttttca
    23521 tcagagtttt ctctcgcctg attacaaatt tgtcgtctta aaaagtgata caggttgcgc
    23581 cctgttcggc accggacagt ttttcacgca aggcgctgaa taattcacgt cctgtcccca
    23641 cgcgtgacgc gctcaggtca ggaatgtgcg gttcgcgagc agccagttcc gcttcgtcta
    23701 ccagcagcgt cagttcgcct gtctgtccat tcacacgaat gatgtccccg tcgcgcactt
    23761 ttgccagcag cccgccatcg taggcttctg gtgttacgtg gatagctgac ggcactttac
    23821 ctgaagcgcc ggagagtcgt ccatcggtaa ctaacgcaat tttgaaacac cggtccaata
    23881 atacaccaag tggcggcatg agtttatgta attctggcat tccgttcgct tttggcccct
    23941 gatgacggac aacaacgaca caatcgcggt ccagcaaacc cgcttcaaag gccggcataa
    24001 cgtcatgctg gctttcaaaa acaaccgctg gcgcttcaat cacctggttc tcaaccggca
    24061 cggcagaggt tttcataacc gcacggccca ggttaccgct taacactttt gtcccaccat
    24121 gatgagagaa aggttgttcg aaggaagcga tcacattgct gtcgagtgat ttttccgccc
    24181 cttcccgcca gtccagttca ccattattca gccatggttc aagggtataa cgagacagac
    24241 caaagcctgc caccgtattg acatcttcat gcagcaggcc tgctttgagc agttcacgca
    24301 ccagaaccgg tacgccacct gccgcctgga agtggttaat atcggccgga ccgttcgggt
    24361 agagacgagc catcagcggt acaacatcag aaaggtcaga gaagtcatcc cagttaatct
    24421 gaataccggc cgcgcgcgcc atcgccacca ggtgcatggt gtggttagtg gaaccaccgg
    24481 tcgccagcag tgcaacgata ccgttcacca ccactttctc atcgatcatc ttaccgatcg
    24541 gcatccattc attaccatta ccggtcatgc gtgtaacctg acgcgcagcg gcagcggtca
    24601 aagcatcgcg cagcggagaa tccggatgaa caaaagaaga gcctggcaac tgcatcccca
    24661 taaactccac caccatctgg ttggtgttgg cagtaccgta gaaagtacat gttcccggcg
    24721 catggtaaga cgcggcttct gactccagta aggccatgcg gtccacttta ccttcggcat
    24781 aaagctggcg aatacgcact ttttctttat ttggcaaacc gcttgccatc ggtccagacg
    24841 gcacaaacac cgcaggcaaa tgaccaaacg acagggctgc catcgtcaga cccgggacaa
    24901 tcttgtcgca caccccgagg aacagagcac catcaaacat gttatgggac agcccgaccg
    24961 ccgcagacat cgctatcact tcgcggctta gcagcgacaa ctccattcca tcctgcccct
    25021 gggtgacacc atcacacatc gccggaacac cgcccgcaac ctgaccaacc gcattcgctt
    25081 catgcagggc tttacgaatg atttctggat agtgttcata aggctggtga gcggagagca
    25141 tgtcgttata ggaggtgatg atggcgatat tgttacgcaa catgcttttc aaagaggctt
    25201 tgtcttctgg ctggcaggca gcgaaaccgt gtgccaggtt accgcatgcc aactgcgaac
    25261 gatgaacggt cgacgttttc gcttgttcta tccgggcgag ataagcagag cgagtctcgc
    25321 gcgaacgttc aatgattcga tttgttacgc gtaacaattg tggattcata aaggctcctg
    25381 aaaatgagtt gtcagagcag gatgattcac aatgcgtttc attcagagga tttatgagtg
    25441 aaacgcctgt aaccggagct catagggcaa aaacgtttca gtcagtgtaa taaaaaaagc
    25501 ctcgtgggtg aatccgcacg aggcctgaaa gtgtaaaaat tgttctacaa tctgtgcaag
    25561 atcatgttac cggtaaaata accataaagg ataagcgcag atattactca aactcattcc
    25621 aggaacgacc atcacgggta atcatcgcca ccgaggcaac gggtccccag gttccggcct
    25681 gatacggttt cggcgcatca ttgtccatcg cccacgcctc agtaatggag tctacccatt
    25741 tccaggcttc ttccacttcg tcgcgacgta caaacagtgc ctgaatacca cgcatggttt
    25801 ccagcagcaa acgttcatag gcatccgcca gatgcgtctg attaaaggtt tctgaatagc
    25861 tcagatccag cttggtgatt tgcaggttat gtttgtggtc aaggccagga actttattca
    25921 gtacctggat atccacgcct tcatcaggtt gcagacggat agtcagttta ttctgcggca
    25981 gatcctgcca cgactcttta aacagattca gttcaggtgt tttgaaatag accacgactt
    26041 cagaacattt ggtcggcaga cgtttaccag tacgcaggta gaatggcaca ccggcccagc
    26101 gccagttatc aatgtcgacg cggatcgcca cgaaggtttc tgtattgctg ctcttgttcg
    26161 cgccctcttc ttccagatat cccggcactt ttttgccctg ggcgaagccc gcagtatatt
    26221 gcccgcgtac ggttttttcg cgtacgttgg agcggtcgat gcggcgcaga gacttcagta
    26281 ctttcacttt ttcatcgcgg atgctgtctg cgctcaggtc agacggcgga gacatcgcaa
    26341 tcatgcaaag aatttgcagc aggtggttct ggatcatgtc gcgcatctga ccggctttat
    26401 caaaatagcc ccagcgccct tcgatcccca cttcttctgc cacggtaatc tcaacatgat
    26461 caatggtgcg attgtcccag ttattcacaa acagggagtt agcaaaacgc agcgccaaca
    26521 ggttcagcac cgtttcttta ccaagatagt ggtcgatacg gtaaacctgg cactcctcga
    26581 agtattcgcc aacctgatca ttgatttcct gcgaggtcgc cagcgacgtc cccagcggtt
    26641 tctccatgac tacgcgtgcc ggtttagcat tcagttttgc ctcgccaagc cctttgcaaa
    26701 ttgcgccaaa agtgctgggc ggcatggcaa agtagttaat ggtgatacga tttttttgat
    26761 ccagcatcgc accgagacgg ttgaatgcag cagtgtcatt gacatcgaga ttacaaaaat
    26821 ccagacgtgc actcagggtg tcccataaac cttcatcaat ggtttctttc atgaaagttt
    26881 cgagcgcctc gcggacaact ttggtatatg ccgctttatc ccagtcagca cgccctacgc
    26941 cgataatccg ggtgtccggg ttgagctgac cggctttttc cagttgatac agggaaggca
    27001 gcaatttacg acgcgcaagg tcgcctttcg cgccgaaaat gaccaggtca caggcctggg
    27061 ctgtttgcgt taccgccatg tcattctcct taagttaact aacccggtac ttaagccagg
    27121 gtatacttgt aattttctta cggtgcactg tactgctttt acgagcttgc gaaaactgta
    27181 aacgcttatc cacccgtgcg attacggtaa aagcgcgcaa agtgcggcaa aactgataaa
    27241 aaaatcatcg ttttccattg ggtaaaaatc tgacactgat catgttatga aaaaaaataa
    27301 caactttttt atctgctttt gtcattaaca cagcacacag gcgtaatatc tgacaaaact
    27361 gcatttcgat ttctttcagt gcggaaatcg tcattacccg tgagtctctt tacatcatga
    27421 atatgctgga aaaaatccag tctcagctgg aacatttgag caaatcagag cgcaaagttg
    27481 ccgaggtcat tctggcttcg cccgataacg cgatccattc gagtattgct gctctggcac
    27541 tggaagccaa tgttagcgaa ccgacggtga atcgtttctg tcgcagcatg gacacgcgcg
    27601 gttttcctga ttttaaactt catctggcac aaagtctggc gaatggcact ccctatgtta
    27661 atcgcaacgt caatgaagat gacagcgttg aatcatacac ggggaaaata tttgagtccg
    27721 caatggcaac gctcgatcat gtccgtcatt cactggataa atctgccatc aaccgcgccg
    27781 tcgacttgct cactcaggca aaaaaaatcg cctttttcgg attaggctct tcagccgccg
    27841 ttgcccacga tgcgatgaat aagttctttc gttttaatgt tccggtggtg tactccgatg
    27901 atatcgtgct gcaacgcatg agttgtatga attgtagcga cggagacgtg gtggtgctga
    27961 tttctcacac tggaagaaca aaaaatctgg tcgagctggc gcagctggca cgcgaaaacg
    28021 acgctatggt gattgccctc acctctgcgg gtaccccgct cgcccgggaa gcaacgctgg
    28081 caattaccct cgacgtaccg gaagatactg acatttatat gcccatggtt tctcgacttg
    28141 cacagctgac cgtgatagat gtgctggcga caggatttac tttgcgacgc ggtgcaaaat
    28201 tcagagataa cttgaagcgg gtcaaggaag cgctgaagga atcgcgtttt gataagcagt
    28261 tacttaattt aagtgacgat cgctaaaagc gactgtcact gtcctaatct tatacgacat
    28321 ccgaatgaga ttaatttatc gccatcgcgg cgttatttca ttcggatttc atgttcaagc
    28381 aacacctggt tgtttcagtc aacggagtat tacatgtcca gaaggcttcg cagaacaaaa
    28441 atcgttacca cgttaggccc agcaacagat cgcgataata accttgaaaa agttatcgcg
    28501 gcgggtgcca acgttgtacg tatgaacttt tctcacggct cgcctgaaga tcacaaaatg
    28561 cgcgcggata aagttcgtga gattgccgca aaactggggc gtcatgtggc tattctgggt
    28621 gacctccagg ggcccaaaat ccgtgtatcc acctttaaag aaggcaaagt tttcctcaat
    28681 attggggata aattcctgct cgacgccaac ctgggtaaag gtgaaggcga caaagaaaaa
    28741 gtcggtatcg actacaaagg cctgcctgct gacgttgtgc ctggcgacat cctgctgctg
    28801 gacgatggtc gcgtccagtt aaaagtactg gaagttcagg gcatgaaagt gttcaccgaa
    28861 gtcaccgtcg gtggtcccct ctccaacaat aaaggtatca acaaacttgg cggcggtttg
    28921 tcggctgaag cgctgaccga aaaagacaaa gcagacatta agactgcggc gttgattggc
    28981 gtagattacc tggctgtctc cttcccacgc tgtggcgaag atctgaacta tgcccgtcgc
    29041 ctggcacgcg atgcaggatg tgatgcgaaa attgttgcca aggttgaacg tgcggaagcc
    29101 gtttgcagcc aggatgcaat ggatgacatc atcctcgcct ctgacgtggt aatggttgca
    29161 cgtggcgacc tcggtgtgga aattggcgac ccggaactgg tcggcattca gaaagcgttg
    29221 atccgtcgtg cacgtcagct taaccgcgcg gtaatcacgg cgacccagat gatggagtca
    29281 atgattacta acccgatgcc gacgcgtgca gaagtcatgg acgtagcaaa cgccgttctg
    29341 gatggtactg acgctgtgat gctgtctgca gaaactgccg ctgggcagta tccgtcagaa
    29401 actgttgcag ccatggcgcg cgtttgcctg ggtgcggaaa aaatcccgag catcaacgtt
    29461 tctaaacacc gtctggacgt tcagttcgac aatgtggaag aagctattgc catgtcagca
    29521 atgtacgcag ctaaccacct gaaaggcgtt acggcgatca ttaccatgac cgaatcgggt
    29581 cgtaccgcgc tgatgacctc ccgtatcagc tctggtctac caattttcgc catgtcgcgc
    29641 catgaacgta cgctgaacct gactgctctc tatcgtggcg ttacgccggt gcactttgat
    29701 agcgctaatg acggcgtagc agctgccagc gaagcggtta atctgctgcg cgataaaggt
    29761 tacttgatgt ctggtgacct ggtgattgtc acccagggcg acgtgatgag taccgtgggt
    29821 tctactaata ccacgcgtat tttaacggta gagtaagtac gttgccggat gcggcgaaaa
    29881 cgccacatcc ggcctacagt tcaatgatag ttcaacagat ttcgaatatt ctgaagcaaa
    29941 cttgaactta tcatcaggcg aaggcctctc ctcgcgagag gcttttttat ttgatgggat
    30001 aaagatcttt gcgcttatac ggctggattt cgcccggttt gcgagttttc agcaatttta
    30061 gtatccaggt gtattgttct ggtcgcggac caacaaaaat ctcgacttct tcattcatcc
    30121 gccgcgcaat cgtatgatca tccgcctcta acagatcatc catcggtggg cgcacctgaa
    30181 tcatcagacg atgcgtcttg ccatcataaa tcggaaacag cggtacaacg cgcgcacggc
    30241 acactttcat caaacgacca atcgcgggca acgtcgcttt ataggtggca aagaaatcca
    30301 caaattcgct gtgctctggg ccatgatcct gatcgggtaa ataatatccc cagtacccct
    30361 gacgtaccga ctggatgaat ggtttaatac cgtcatttct cgcatgcaga cgaccgccaa
    30421 agcgacgacg caccgtgttc cagacataat caaaaaccgg gttgccctga ttatggaaca
    30481 tcgctgccat tttctgccct tgcgaggcca tcagcatggc aggaatatcg acggcccaac
    30541 cgtgcggcac cagaaagata actttctcgt tattacgccg catctcttcg atgatctcca
    30601 gcccttgcca gtcaacgcgc ggctgaattt tctccggccc gcgtattgcc aactcagcca
    30661 tcattgccat cgcttgcggc gcggtggcaa acatctcatc aacaatcgct tcgcgttcag
    30721 cttcactacg ttctggaaag cagagcgaca gattgattaa cgcacgacgg cgtgagcttt
    30781 ttcccagtcg tccggcaaaa cgtcccagcc gtgccagaat gggatcacgg aactttggcg
    30841 gcgttaaagc gatacccgcc atcgctgcta cgcccagcca tgctccccag tagcgcgggt
    30901 ggcgaaagga tttatcaaac tcaggaatgt attcgctatt attttttttc gtttccatgc
    30961 ttttccagtt tcggataagg caaaaatcaa tctggtgata gtgtagcggc gcaacttgcc
    31021 ccgcaccaaa taaaaaaagc cggtactgac tacgtaccgg ctgcgaatgg atgttaatta
    31081 atcaaaccgt agctgcggca caatctcttt ggcctgcgcc aggaattcgc gacgatcgga
    31141 gccggtcagc ccttcggtac gcggcagttt tgccgtcagc gggtttacgg cctgctggtt
    31201 tatccatact tcatagtgca gatgcggccc ggttgaacgt ccggtattac cggaaagcgc
    31261 gatacggtcg ccacgtttca ccttctgtcc cggtttcacc agaatcttgc gcaagtgcat
    31321 ataacgcgtg gtgtagctgc gaccatgacg aatagccaca taataacctg ctgcgccact
    31381 gcgtttggca accaccactt caccgtcacc cactgaaagc actggcgtac cctgcggcat
    31441 ggcgaaatca acacctctgt gtggtgcaac gcgaccggtc accggattag tacgacgcgg
    31501 gttaaagtta gacgagatac ggaactgttt cgccgtcggg aatcgcaaga atcctttcgc
    31561 cagaccagta ccgttacgat cgtagaattt gccatcttca gcgcggatag cgtaataatc
    31621 tttaccttct gaacgcaaac gtacgcccag cagctggctt tgctcacgtt taccatcaag
    31681 catttctcga gacattaata ccgcaaattc atcgcctttt ttcagtttgc ggaaatccat
    31741 ttgccactgc atggctttaa tcactgcgct cacttcggcg ctggttaaac cggcgtttct
    31801 ggcgctggca acaaagctcc ccccgacggt acctttcagc agattgttaa cccactctcc
    31861 ttgctgcatt tcgctggtca ttttaaaacc gttagcggca gtacggtcat aggttcgggt
    31921 ttcacgacga gacacttccc aggtgaggcg ctgcagttcg ccgtccgcgg ttaatgtcca
    31981 ggagagttgt tgaccgattt tcaggttacg caattctttg tcggccgcag ccagttgggt
    32041 gatatcaccc atatcaatac catactgatt gagaatgctg cttagcgtat cgccagtgga
    32101 aacaacatat tcatgcacgc ccgcttcacc ggcgattttg tcatccagtt cgtcctgggg
    32161 aatggcttca tcttcttgtg cagcttgatc aatcggctca ctggcttcag gtaagagcga
    32221 acgaatttcg ttctgttcca gctcaatggt tttgacaatt ggcgtggcat cgcggtgata
    32281 aacatagggc cgccagacag cgacggccag agtaagaacg gtgagcgacc ccaacataac
    32341 gcggtgtggt cgcggtaaat tattaaacgc cagggcgaca gagcgggcta tctgttgcac
    32401 gtaatcactt cctcattaat ctcctttcag gcagctcgca tactggttgg ctaattgact
    32461 caggaattct gaatagcttg ttttacccag tttgatattc gtccccaggg gatccaacgt
    32521 tcccatacga acggatgtcc ctcgtgcgac gctctcaacg accgctggcc tgaactgtgg
    32581 ctcagcaaaa acgcaggttg ctttttgctc aaccaactgt gttcttattt catgtaaacg
    32641 ctgcgcgcca ggttgaatct cagggttaac ggtaaaatga ccaagcggtg tcagtccgaa
    32701 ctgtttttcg aaatagccgt aagcatcgtg aaaaacgaaa taacctttcc ccttgagcgg
    32761 cgcgagctcg ttaccaacct gcgtttcggt tgaggctaat tgtgcctcaa aatccttcag
    32821 gttggcgtca agtttggctc gactttgcgg cataagttcc actaattttc catggattgc
    32881 aaccgctgta gcccgcgcta tctctgggga aagccaaaga tgcatgttga aatcgccgtg
    32941 atggtgatct tcgtcacttt tttccgcgtg gtcgtgatca tcatcatcgc cgtgaatact
    33001 tttcatcagt agcggtttca catcttctag ctgcgcaatc gttacctgct tcgcttctgg
    33061 taatttgctt accggttttt gcataaacgc ttccatctcc gggccaaccc aaacgactaa
    33121 gtccgcgttc tgtaagcgtt ttacatccga tggacgcagt gaataatcat gttctgaagc
    33181 accgtcaggc agtaaaacct ccgtttctgt taccccatca gcaatggcag aagcgatgaa
    33241 cccaacgggt ttaagcgaag cgacaacggc ggcatctgcg gcctgtgttg cacctcccca
    33301 gagagcggcg gataatgctg cgaaaagaag cgttttttta tgtaacataa tgcgaccaat
    33361 catcgtaatg aatatgagaa gtgtgatatt ataacatttc atgactactg caagactaaa
    33421 attaacatga caagtctggt ttccctggaa aatgtctcgg tttcttttgg ccaacgccgc
    33481 gtcctctctg atgtgtcgct ggaacttaaa cctggaaaaa ttttgacttt acttgggcca
    33541 aatggcgcag gtaagtcgac actggtacgg gtagtgctcg ggctggtaac acccgatgaa
    33601 ggggttatca agcgcaacgg aaaactgcgc atcggctatg taccgcagaa gctgtatctc
    33661 gacaccacgt tgccactgac cgtaaaccgt tttttacgct tacgccctgg tacacataaa
    33721 gaagatattt tgcctgcact gaaacgtgtc caggccgggc acctgattaa cgcaccgatg
    33781 caaaagctct ctggtggcga aacgcagcgt gtactgttag cgcgagcatt gttaaatcga
    33841 ccgcaattat tagtgctgga tgaacccact caaggcgtgg atgtaaatgg ccaggtggcg
    33901 ttatatgacc ttattgacca actgcgtcgt gaactggatt gtggcgtttt aatggtttct
    33961 cacgatctgc atctggtgat ggcaaaaacc gatgaagtgc tgtgcctgaa tcaccacatt
    34021 tgttgttccg gcacaccgga agttgtttcc ctgcatccgg agtttatttc aatgtttggt
    34081 cctcgtggtg ctgaacaact gggtatctat cgccatcatc ataatcatcg tcacgattta
    34141 cagggacgaa ttgttttgcg tcggggaaat gatcgctcat gattgaatta ttatttcccg
    34201 gttggttagc cgggatcatg ctcgcctgtg ccgcgggtcc gctgggttcg tttgtagtct
    34261 ggcgtcgtat gtcttatttc ggtgatacgc tggctcatgc ctcattactt ggtgtcgcgt
    34321 ttggtttgtt gctggacgtg aatccattct atgcggtgat tgccgttacg ctgctgctgg
    34381 cgggcggtct ggtatggctg gagaagcgtc cacagctggc gatcgacacg ttattaggga
    34441 ttatggcgca cagtgccctg tcgctgggcc tggtggtcgt tagtctgatg tctaacattc
    34501 gtgttgattt gatggcttac ctgttcggtg atttactggc agtgacgcca gaagatctca
    34561 tctctattgc aattggcgtg gtcatcgtgg tggctatttt gttctggcaa tggcgcaatt
    34621 tgctgtcgat gacgattagc ccggatctgg cgtttgttga tggtgtgaaa ttacagcgag
    34681 tgaaattgtt gttgatgctg gtgacggcat tgacgattgg tgtagcgatg aaatttgtcg
    34741 gtgcgttgat tattacttcg ttgctgatta ttcctgcagc tactgcgcgt cgctttgccc
    34801 gtacgccgga acagatggct ggtgtcgctg ttttggtggg gatggtggca gtaactggcg
    34861 gtttaacctt ttccgcgttt tacgatacgc cggcgggtcc gtcggtggtg ctatgtgcgg
    34921 cactgttatt tattatcagt atgatgaaaa agcaggccag ctaatctatc gctgaacaca
    34981 tttgtcggat gcggcgcgag cgccttatcc gacctacggt tcgctatctc tggtaggcct
    35041 ggtaagacgc gaacagcggc gcgagcgcct tttccgccct acggttcgct atctctggta
    35101 tatgcctgat aagacgcgaa tagcgtcgca tcaggcacac tgccaatgtc ggatgcggtg
    35161 cgagcgacca atccgattta cggcatttct ggcggcgtta tgccaaagtg attccacgcc
    35221 cgcgtcgtcg ccatacgccc gcgcggtgta cgctgcaaaa agccttgctg aatcaaataa
    35281 ggttccagca catcctcaat ggtttcacgt tcttcgccaa tagccgccgc caggttatcc
    35341 agacctaccg gcccaccaaa gaacttatcg attaccgcca gcaacaattt gcggtccata
    35401 taatcgaaac cttcagcatc gacattcaac atatccagcg cctgagcagc gatatctgcc
    35461 gagatggtgc catcgtgctt cacttcggcg aaatcacgca ctcgacgcag cagacggttg
    35521 gcaatgcgcg gcgtaccgcg agcgcgacga gcaacttcca gcgcgccgtc atcactcatc
    35581 tcaagcccca taaagcgtgc gctgcgactg acgatatatt gcagatccgg cacctgataa
    35641 aactccagac gttgcacaat accaaaacgg tcgcgcaacg gtgatgtcag cgaacctgcg
    35701 cgcgtggttg caccaatcag ggtaaacggc ggcaaatcaa ttttaatgga gcgtgccgcc
    35761 ggaccttcac caatcatgat atccagttgg tagtcttcca ttgccgggta cagcacttct
    35821 tcaacaactg gcgataaacg gtggatctca tcaataaaca gcacgtcatg cggttcaagg
    35881 ttagtgagca tcgcagccaa atcgcccgcc ttttccagca ccggaccaga agtcgtgcgt
    35941 aaattaacgc ccatttcatt ggcgacaatg ttggcaagcg tagttttacc caaccccgga
    36001 ggaccaaaaa tcaacaaatg atcgagggca tcgccgcgca gtttcgctgc tttgatgaaa
    36061 atctccatct gtgaacgaac ctgcggctga ccaacatact cttccagtaa tttagggcga
    36121 atggcgcgat ctgctacatc ttccggcaaa gtggtaccgg cagaaatcag acggtctgct
    36181 tcaatcatcc tttacctcat aacgcggcgc gtagggcttc gcgaattaat gtttcactgc
    36241 tggcgtcagg gcgagcgatt ttgctcacca tgcggcttgc ttcttgtggt ttatagccca
    36301 gcgccaccag cgcggcaacc gcttcttgtt cagcatcgtc ggtcgccggg ctggcaggag
    36361 acgtgagtac caggtcggcg gctggcgtaa agagatcgcc atgcaaacct ttaaatcggt
    36421 ctttcatttc aacaatcaag cgttcggcgg tttttttgcc aatacccggc agtttcacca
    36481 gtgcccccac ttcttcacgc tcaacggcat taacgaactg ctgcgctgac attccggaga
    36541 ggatcgccag cgccaacttc gggccgacgc cgttggtttt gatcaactct ttaaacaatg
    36601 tgcgctcttg tttattgtta aaaccgtaca gcagttgcgc gtcttcacgc accacaaagt
    36661 gggtgaagac gatcgcttcc tgacccgctt cagggagttc ataaaaacag gtcatcggca
    36721 tatgcacttc atagcctacg ccgcccactt caattaacac cagcgggggt tgtttttcaa
    36781 tgatgatgcc tctgagtctg cctatcacat gacgctcctg cgtaatgaat caaagataat
    36841 gctgtatgat aaaaaaatgc tggatagata tccagcgaag gatgaagaaa acttgcgagg
    36901 tgtctcgatg aactg
//
LOCUS       DAZKFU020000013        35189 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZKFU020000013 DAZKFU020000000
VERSION     DAZKFU020000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.013"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..832)
                     /gene="rlmB"
                     /locus_tag="ACHOQQ_RS02670"
                     /old_locus_tag="pgaptmp_000534"
     CDS             complement(101..832)
                     /gene="rlmB"
                     /locus_tag="ACHOQQ_RS02670"
                     /old_locus_tag="pgaptmp_000534"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGU4468839.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1013..3454)
                     /gene="rnr"
                     /locus_tag="ACHOQQ_RS02675"
                     /old_locus_tag="pgaptmp_000535"
     CDS             complement(1013..3454)
                     /gene="rnr"
                     /locus_tag="ACHOQQ_RS02675"
                     /old_locus_tag="pgaptmp_000535"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGU4468840.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3493..3918)
                     /gene="nsrR"
                     /locus_tag="ACHOQQ_RS02680"
                     /old_locus_tag="pgaptmp_000536"
     CDS             complement(3493..3918)
                     /gene="nsrR"
                     /locus_tag="ACHOQQ_RS02680"
                     /old_locus_tag="pgaptmp_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGU4468841.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4123..5421)
                     /gene="purA"
                     /locus_tag="ACHOQQ_RS02685"
                     /old_locus_tag="pgaptmp_000537"
     CDS             complement(4123..5421)
                     /gene="purA"
                     /locus_tag="ACHOQQ_RS02685"
                     /old_locus_tag="pgaptmp_000537"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGU4468842.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKERSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5525..5722)
                     /gene="yjeT"
                     /locus_tag="ACHOQQ_RS02690"
                     /old_locus_tag="pgaptmp_000538"
     CDS             complement(5525..5722)
                     /gene="yjeT"
                     /locus_tag="ACHOQQ_RS02690"
                     /old_locus_tag="pgaptmp_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGU4468843.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5804..6808)
                     /gene="hflC"
                     /locus_tag="ACHOQQ_RS02695"
                     /old_locus_tag="pgaptmp_000539"
     CDS             complement(5804..6808)
                     /gene="hflC"
                     /locus_tag="ACHOQQ_RS02695"
                     /old_locus_tag="pgaptmp_000539"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGU4468844.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6811..8070)
                     /gene="hflK"
                     /locus_tag="ACHOQQ_RS02700"
                     /old_locus_tag="pgaptmp_000540"
     CDS             complement(6811..8070)
                     /gene="hflK"
                     /locus_tag="ACHOQQ_RS02700"
                     /old_locus_tag="pgaptmp_000540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGU4468845.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8156..9436)
                     /gene="hflX"
                     /locus_tag="ACHOQQ_RS02705"
                     /old_locus_tag="pgaptmp_000541"
     CDS             complement(8156..9436)
                     /gene="hflX"
                     /locus_tag="ACHOQQ_RS02705"
                     /old_locus_tag="pgaptmp_000541"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGU4468846.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9513..9821)
                     /gene="hfq"
                     /locus_tag="ACHOQQ_RS02710"
                     /old_locus_tag="pgaptmp_000542"
     CDS             complement(9513..9821)
                     /gene="hfq"
                     /locus_tag="ACHOQQ_RS02710"
                     /old_locus_tag="pgaptmp_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGU4468847.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9907..10857)
                     /gene="miaA"
                     /locus_tag="ACHOQQ_RS02715"
                     /old_locus_tag="pgaptmp_000543"
     CDS             complement(9907..10857)
                     /gene="miaA"
                     /locus_tag="ACHOQQ_RS02715"
                     /old_locus_tag="pgaptmp_000543"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGU4468848.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10850..12697)
                     /gene="mutL"
                     /locus_tag="ACHOQQ_RS02720"
                     /old_locus_tag="pgaptmp_000544"
     CDS             complement(10850..12697)
                     /gene="mutL"
                     /locus_tag="ACHOQQ_RS02720"
                     /old_locus_tag="pgaptmp_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGU4468849.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPTPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPHEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12707..14044)
                     /gene="amiB"
                     /locus_tag="ACHOQQ_RS02725"
                     /old_locus_tag="pgaptmp_000545"
     CDS             complement(12707..14044)
                     /gene="amiB"
                     /locus_tag="ACHOQQ_RS02725"
                     /old_locus_tag="pgaptmp_000545"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGU4468850.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14063..14524)
                     /gene="tsaE"
                     /locus_tag="ACHOQQ_RS02730"
                     /old_locus_tag="pgaptmp_000546"
     CDS             complement(14063..14524)
                     /gene="tsaE"
                     /locus_tag="ACHOQQ_RS02730"
                     /old_locus_tag="pgaptmp_000546"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGU4468851.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14496..16043)
                     /gene="nnr"
                     /locus_tag="ACHOQQ_RS02735"
                     /old_locus_tag="pgaptmp_000547"
     CDS             complement(14496..16043)
                     /gene="nnr"
                     /locus_tag="ACHOQQ_RS02735"
                     /old_locus_tag="pgaptmp_000547"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGU4468852.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQDSDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIASLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTEKNHD
                     ESSNSAP"
     gene            16042..17181
                     /gene="queG"
                     /locus_tag="ACHOQQ_RS02740"
                     /old_locus_tag="pgaptmp_000548"
     CDS             16042..17181
                     /gene="queG"
                     /locus_tag="ACHOQQ_RS02740"
                     /old_locus_tag="pgaptmp_000548"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGU4468853.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17164..17217)
                     /locus_tag="ACHOQQ_RS02745"
                     /old_locus_tag="pgaptmp_000549"
     CDS             complement(17164..17217)
                     /locus_tag="ACHOQQ_RS02745"
                     /old_locus_tag="pgaptmp_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4468854.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17451..17526)
                     /locus_tag="ACHOQQ_RS02750"
                     /old_locus_tag="pgaptmp_000550"
     tRNA            complement(17451..17526)
                     /locus_tag="ACHOQQ_RS02750"
                     /old_locus_tag="pgaptmp_000550"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17491..17493),aa:Gly,seq:gcc)
     gene            complement(17562..17637)
                     /locus_tag="ACHOQQ_RS02755"
                     /old_locus_tag="pgaptmp_000551"
     tRNA            complement(17562..17637)
                     /locus_tag="ACHOQQ_RS02755"
                     /old_locus_tag="pgaptmp_000551"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17602..17604),aa:Gly,seq:gcc)
     gene            complement(17674..17749)
                     /locus_tag="ACHOQQ_RS02760"
                     /old_locus_tag="pgaptmp_000552"
     tRNA            complement(17674..17749)
                     /locus_tag="ACHOQQ_RS02760"
                     /old_locus_tag="pgaptmp_000552"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17714..17716),aa:Gly,seq:gcc)
     gene            complement(17960..18505)
                     /gene="orn"
                     /locus_tag="ACHOQQ_RS02765"
                     /old_locus_tag="pgaptmp_000553"
     CDS             complement(17960..18505)
                     /gene="orn"
                     /locus_tag="ACHOQQ_RS02765"
                     /old_locus_tag="pgaptmp_000553"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU4468855.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18600..19652
                     /gene="rsgA"
                     /locus_tag="ACHOQQ_RS02770"
                     /old_locus_tag="pgaptmp_000554"
     CDS             18600..19652
                     /gene="rsgA"
                     /locus_tag="ACHOQQ_RS02770"
                     /old_locus_tag="pgaptmp_000554"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU4468856.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19749..20717
                     /gene="asd"
                     /locus_tag="ACHOQQ_RS02775"
                     /old_locus_tag="pgaptmp_000555"
     CDS             19749..20717
                     /gene="asd"
                     /locus_tag="ACHOQQ_RS02775"
                     /old_locus_tag="pgaptmp_000555"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU4468857.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20739..24062
                     /gene="mscM"
                     /locus_tag="ACHOQQ_RS02780"
                     /old_locus_tag="pgaptmp_000556"
     CDS             20739..24062
                     /gene="mscM"
                     /locus_tag="ACHOQQ_RS02780"
                     /old_locus_tag="pgaptmp_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU4468858.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAGLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24213..25715)
                     /gene="yjeM"
                     /locus_tag="ACHOQQ_RS02785"
                     /old_locus_tag="pgaptmp_000557"
     CDS             complement(24213..25715)
                     /gene="yjeM"
                     /locus_tag="ACHOQQ_RS02785"
                     /old_locus_tag="pgaptmp_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU4468859.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(25934..26911)
                     /gene="epmA"
                     /locus_tag="ACHOQQ_RS02790"
                     /old_locus_tag="pgaptmp_000558"
     CDS             complement(25934..26911)
                     /gene="epmA"
                     /locus_tag="ACHOQQ_RS02790"
                     /old_locus_tag="pgaptmp_000558"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU4468860.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27236..29044
                     /gene="frdA"
                     /locus_tag="ACHOQQ_RS02795"
                     /old_locus_tag="pgaptmp_000559"
     CDS             27236..29044
                     /gene="frdA"
                     /locus_tag="ACHOQQ_RS02795"
                     /old_locus_tag="pgaptmp_000559"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU4468861.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29037..29771
                     /gene="frdB"
                     /locus_tag="ACHOQQ_RS02800"
                     /old_locus_tag="pgaptmp_000560"
     CDS             29037..29771
                     /gene="frdB"
                     /locus_tag="ACHOQQ_RS02800"
                     /old_locus_tag="pgaptmp_000560"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4468862.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29782..30177
                     /gene="frdC"
                     /locus_tag="ACHOQQ_RS02805"
                     /old_locus_tag="pgaptmp_000561"
     CDS             29782..30177
                     /gene="frdC"
                     /locus_tag="ACHOQQ_RS02805"
                     /old_locus_tag="pgaptmp_000561"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4468863.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30188..30547
                     /gene="frdD"
                     /locus_tag="ACHOQQ_RS02810"
                     /old_locus_tag="pgaptmp_000562"
     CDS             30188..30547
                     /gene="frdD"
                     /locus_tag="ACHOQQ_RS02810"
                     /old_locus_tag="pgaptmp_000562"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4468864.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            30610..31743
                     /gene="ampC"
                     /locus_tag="ACHOQQ_RS02815"
                     /old_locus_tag="pgaptmp_000563"
     CDS             30610..31743
                     /gene="ampC"
                     /locus_tag="ACHOQQ_RS02815"
                     /old_locus_tag="pgaptmp_000563"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4468865.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            31832..32365
                     /gene="blc"
                     /locus_tag="ACHOQQ_RS02820"
                     /old_locus_tag="pgaptmp_000564"
     CDS             31832..32365
                     /gene="blc"
                     /locus_tag="ACHOQQ_RS02820"
                     /old_locus_tag="pgaptmp_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4468866.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32362..32679)
                     /gene="sugE"
                     /locus_tag="ACHOQQ_RS02825"
                     /old_locus_tag="pgaptmp_000565"
     CDS             complement(32362..32679)
                     /gene="sugE"
                     /locus_tag="ACHOQQ_RS02825"
                     /old_locus_tag="pgaptmp_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4468867.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(32855..33001)
                     /gene="ecnB"
                     /locus_tag="ACHOQQ_RS02830"
                     /old_locus_tag="pgaptmp_000566"
     CDS             complement(32855..33001)
                     /gene="ecnB"
                     /locus_tag="ACHOQQ_RS02830"
                     /old_locus_tag="pgaptmp_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4468868.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33112..33237)
                     /gene="ecnA"
                     /locus_tag="ACHOQQ_RS02835"
                     /old_locus_tag="pgaptmp_000567"
     CDS             complement(33112..33237)
                     /gene="ecnA"
                     /locus_tag="ACHOQQ_RS02835"
                     /old_locus_tag="pgaptmp_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4468869.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33289..33855)
                     /gene="efp"
                     /locus_tag="ACHOQQ_RS02840"
                     /old_locus_tag="pgaptmp_000568"
     CDS             complement(33289..33855)
                     /gene="efp"
                     /locus_tag="ACHOQQ_RS02840"
                     /old_locus_tag="pgaptmp_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4468870.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            33897..34925
                     /gene="epmB"
                     /locus_tag="ACHOQQ_RS02845"
                     /old_locus_tag="pgaptmp_000569"
     CDS             33897..34925
                     /gene="epmB"
                     /locus_tag="ACHOQQ_RS02845"
                     /old_locus_tag="pgaptmp_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4468871.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVITSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8359 a         9639 c         8975 g         8216 t
ORIGIN      
        1 aatattaaca aaatgaatat ttgcagtacg taattatctt accagctata gacaaaaaaa
       61 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgcaccg
      121 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      181 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      241 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      301 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      361 caaggttagt cacccgaatc agtggaacgc tttctgccgc gccgcaggct acttttttcg
      421 ccgtggcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcgtga acgccagcgg
      481 cgtccgcgct acgcaggcac gcgccgaggt tgtgcggatc ggttacaccg tcgaggatca
      541 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      601 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      661 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      721 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      781 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      841 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      901 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      961 aaagcgcatt ttgtcagcaa tctaaccctc ttctgtttga agaggatatt gatcactctg
     1021 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg
     1081 ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc tttcggcttc gtttttttct
     1141 caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga cgacgcttgc
     1201 cgcctttttt gcctgcatcg ccttttttcg ctttctcacg cgccgtttta ccgacgttgc
     1261 gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt gcgctcgtcc atattaaccg
     1321 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattcaccca
     1381 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
     1441 ccagcccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
     1501 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
     1561 cagccacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
     1621 gttgcagcat ctcttccatc gaataatggt agccaccggt ttcagtggtg ttgccctgat
     1681 gcccctgctc tttcgccaac agatacttaa tggcgcggtg cagcgtcagg tcaggataac
     1741 gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc caggccaaag tgaccacggt
     1801 tttccggatc gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc agcatttctg
     1861 catcaggacg gtcggcaacc gactccagca gctccgcgta gtcgcgcggt tccggcttgt
     1921 taccgcccgg cagttccagc cccagctctg ccagcactga acggaaagag gtaatcgctt
     1981 cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac
     2041 gcgccgccga gatattcgcc agaatcatgc actcttcaat taacttgtgt gcgtcgttac
     2101 gctgagtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt
     2161 cgctctcaaa tgagatcccg ccgcgttctt cacgggcttt atccagcact ttatagaggt
     2221 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
     2281 cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt
     2341 cgtagaattt gtagccagtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
     2401 acaggcggtc tacctgcgga ttgagcgagc acagaccgtt agagagcact tccggcagca
     2461 tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca
     2521 gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
     2581 caccaccgcg ttttttctcg cagtaaaccg catcgtcaaa gtcacgggcg tcttcgccat
     2641 caatggtaac cagcggtaaa tcgcgcagat cgacacggcc cgcttttgct tcttccggca
     2701 cttcttcttt cagcccggca acctgttgct caacagcctg cggccagatg tacggaattt
     2761 catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt
     2821 cgacgatttt acccaccgct ttggtacggc gagtcgggcg ttgggtcagt tcgactacga
     2881 ccacaaagcc catccgcgcg cccataatct gatcgggcgg gattaaaata tcgaagctca
     2941 gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
     3001 tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt
     3061 cagcgcccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
     3121 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
     3181 ggccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
     3241 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
     3301 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
     3361 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
     3421 cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga
     3481 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
     3541 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
     3601 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
     3661 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccaa
     3721 gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
     3781 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
     3841 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
     3901 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
     3961 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
     4021 cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca
     4081 ctgttagccc agcaggcaaa atatctgcca ggcgtaccaa aattacgcgt cgaacgggtc
     4141 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
     4201 ggtcagctct tcaatacgtt tgatgtagtt cagcgccgcc tgcggcagac cgctacgttc
     4261 tttcacgccg aaggtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
     4321 tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta
     4381 agccacacag agtttcacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa
     4441 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
     4501 acgacgacga cgacccgtag ttgcgccgaa ttcgttaccc tgcttgcaga ggaactcgcc
     4561 agtttcatca aacagttcag tcgggaacgg acctgcaccc acacgagtgg agtaagcttt
     4621 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
     4681 accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag
     4741 cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
     4801 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
     4861 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
     4921 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
     4981 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
     5041 gatcgctttc gcgccacgcg ctttctcacg cgcgttatca agcgcaacgt gataatcaag
     5101 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
     5161 cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat
     5221 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
     5281 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
     5341 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
     5401 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
     5461 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
     5521 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
     5581 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
     5641 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
     5701 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
     5761 agaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
     5821 tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat
     5881 tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat
     5941 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcatga
     6001 tacggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc
     6061 gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
     6121 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
     6181 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
     6241 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgccg
     6301 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
     6361 cgagggtcag acgaccacgg gaatcagtga cgatatcttt cacgtccagg cgaccaattt
     6421 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
     6481 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
     6541 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
     6601 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagacccg
     6661 gctcataaac cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
     6721 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
     6781 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
     6841 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
     6901 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
     6961 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
     7021 caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
     7081 cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag
     7141 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
     7201 cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggtata
     7261 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
     7321 cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat
     7381 acccatgtca tacgggcgga tagtctcttc cagttcgcgc tgagtatcgc tacgaatcac
     7441 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc
     7501 gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc
     7561 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
     7621 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
     7681 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
     7741 gcgtgttacc acgccacgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
     7801 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
     7861 ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt
     7921 tttactcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
     7981 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
     8041 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
     8101 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
     8161 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
     8221 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
     8281 gctgataaaa acgacttctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
     8341 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
     8401 tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt
     8461 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
     8521 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg
     8581 catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
     8641 ctgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt
     8701 caccgacatc tgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt
     8761 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
     8821 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
     8881 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
     8941 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
     9001 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
     9061 gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa
     9121 ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
     9181 ggctcagggc atggtcaaaa agaacgaccg aagcacccgt cgctttgaca gcttccgcaa
     9241 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
     9301 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
     9361 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
     9421 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
     9481 ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc
     9541 gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc gccaccggca
     9601 ttgttactgt gatgagaaac cgggcgagac gggacaacag tagaaatcgc gtgcttgtaa
     9661 accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga ctcgatttgc
     9721 ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc ccgacgcagt
     9781 gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt tccttatatg
     9841 cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg tctcaattgt
     9901 acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg cctgttctgg
     9961 tttttcacta tcaagccagt gaaccccttc ccaaccacgc agccaggtta tctgccgctt
    10021 cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg agatttcgcc
    10081 ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca agtccgtatg
    10141 caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag ccaacatctg
    10201 atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg cgatggcgaa
    10261 ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt
    10321 acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat
    10381 ccttgctgcc gcaaccggat cgatctcctg aagttgacga tgcaatgact cccaaccttg
    10441 ctctgccgcc tgttgctcaa ttctggcccg tacttccggg tctgccgacg gtagcggcga
    10501 caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca gtgggatccg
    10561 ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat cagcagccga
    10621 gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga gtaactcttc
    10681 agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag aatcaacgct
    10741 tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt taccggaggc
    10801 cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat cactcatctt
    10861 tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt
    10921 tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc tgtgccattg
    10981 accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt
    11041 cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt tgtaaatttt
    11101 gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct gactggaaat
    11161 caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt tcttcgccag
    11221 aaacttttag ccgcaacgga atcagcaacg gctgggcgca aacgggcgct tcacccggcg
    11281 tcagttgtac ctgacgcagc caacgttctg ccactggcaa ggctaaaagt gaaatgttgc
    11341 cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga ccaaaactct
    11401 gactgttcgc cgcaagtgca ggttcatgcg gttctggcgc ttttggtttt tgcatcggcg
    11461 cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtaa cctggttgcg
    11521 cattcggcca gggggcagcc ggacgactgc ctgatgctgg cgcaggagtg tagcgcggag
    11581 ctactggctc acgaactgcc ggttcagcaa agtgattgcg ccccgccgcc acgcggtttt
    11641 ccgggatggc acgcggtgtg ggttgtggtt catcgtccag cggtagcggc gtttccagct
    11701 gctgttgtag cacgctcagc acgccctgat agataaagtc atgcaccagg cgcgactgat
    11761 ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc cacctgatgc gggtcgatct
    11821 ccagatacaa cacaaacgcc ggttgttgat cggcccccag tttgtcttcg caggcctggc
    11881 ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc attcacgtag caatactgaa
    11941 tttccgccag tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgc agcgtgagat
    12001 cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa agcggtgcca caaatcgcgc
    12061 ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactggcgt acgattttgc
    12121 cgttatgcga caggttgatc gtgacgtcga aacgcgccag tgcgatgcgg cggatgattt
    12181 catcgatatg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt
    12241 tgtagaacag gtccagtacc tccagcgtcg ttcccaccgg atgcgccgcc ggtttaaccg
    12301 tcacgtccat atcgcgccct tcggcatagg cctgccaggc ttcctgctgt tctgcggtgc
    12361 gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc
    12421 ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg gcatgacgcg
    12481 ccagcgccag cgccagctcg tcttttttga taccgcagcc gttatcacga atgcggataa
    12541 gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca tcgaggctgt
    12601 tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct gcggcaatct
    12661 ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag tttggcagcg
    12721 tacgatctgg cgtcgtcacc gtactggcag tttgtgccgt tgcaccctgc ggcgcagatt
    12781 gcatcggatg cgcaaggaaa taattgcgca gacctttata aatggcttct gccagctgtt
    12841 gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata aaaccggttt
    12901 cgaccagtac tgatgggata tccggcgaac gcagaacgcc aaggctggcg tgttctggtc
    12961 gacgtttatg aatttcgcca atgcgttgca actgactgat catactggtc gctacatcat
    13021 accctacccg ctgggaatga ccgaactgta aatccagcac agcctggctt aaataggggt
    13081 cagactgact gttcgccagc acatcgcccg ctccgcccag tagctccgac tgtttctcgt
    13141 gctgctccag ccagctggcc atttcgctgt tggcgcgacg gttagagagc acccatacgg
    13201 aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg aatcgacacg aggaaattgg
    13261 cgttttgctt acgtgccaca tcgctgcgcc ccatcaccga gataaagtaa tccccgtcac
    13321 gggttaaaac gcccttaaac atcggatcgt cattgagcaa agtacgcaat ttacgcgcga
    13381 tggcgatggt gacatttttc tcccgcgtac cgccggggcc gatagcgcca gggtcctgac
    13441 cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc gtcgcgcgcg
    13501 ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta ctttccgttt
    13561 taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgtaggc gtttcaacgc
    13621 gtttcgcaac cacaggcggc ggaggaggcg gtggcggcgc atcggcgtta atcgtaaaga
    13681 cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttacca ttttcggtaa
    13741 gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccggagcga atcgccttca
    13801 ccagattatt accgctgaac aacaacggca gtccctgaat cacgcccgtt tgtttgatat
    13861 cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg ccaataaaac
    13921 tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag agcgtcgcgg
    13981 cacccacctg cgcgcacagc agcagcaacg tcgctaccaa ccaatttctg atgcgataca
    14041 tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat tcacccgcag
    14101 aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg tgtatttcga
    14161 catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg cagatggcat
    14221 cgttggcaaa atagtcgcgg atccccataa actccagctc ctcgggatcg gcaaggcggt
    14281 acaaatcaaa gtgataaacc attaagttgt cgagcgtata gggttcgacc agcgtatagg
    14341 tcgggctttt gacattaccc tgatgaccca aagcctgcaa aaagccccgg ctaaaggtgg
    14401 ttttacctgc gcctaaatcg ccatacagat agatgacggt tgcaccatcg caggctttgg
    14461 ctacccgctc gcccaggtct aaggttgcct gctcatcagg gagcggaatt actcgattca
    14521 tcatggtttt tttcagtcac ttccgggtta acaatacgct gtagcgtgga aaagagatcg
    14581 gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc cgcaccgtgc
    14641 gcgacacagc ctgcacaggc tgcatcatac ggcgacagtt tttgcccaag caatgcgcca
    14701 ataataccag agagcacatc gcccatgccg ccgcttgcca tgcctgcatt tccggcatca
    14761 ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt cagcaccgct
    14821 acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact ttcaatttca
    14881 gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat cacgcgattg
    14941 tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca caacatcggt
    15001 ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg gcccagaccg
    15061 ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc catagtcagt
    15121 tcatgcacca tcaattctgg tcgtgcagtc agcagcgacg caatgttctc actgcgggtc
    15181 agtactcgga ctaaaccagc accagcacgc agcgccgctt cccccgtcat acgaatagcc
    15241 cccgccgtgc cgtgatcgcc accgataatc accagccgcc cgtgatcgcc tttatgcgaa
    15301 gtcgggcgac gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg ctgaattttc
    15361 gtctcctgac ctgccagcca actatccaac cccagtgagt caaaatgcag ttgtccggta
    15421 acatcccgcg cttttccagt gagcaagcct ggtttcagcg caataaaagt gatggtgtga
    15481 tcggcgttga tcaccgcacc tggcgtagcg ccggtttcag ccagcaggcc ggaagggata
    15541 tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg gctaatggat
    15601 tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat caacaatcag atctaccgat
    15661 tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca tgcttcgcgt
    15721 gccagcgcgg cctcttccgg caacggtttg tcgctatcct gggccaacaa cgtgacctca
    15781 atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt attaccatga
    15841 ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac ctggaatgcg
    15901 gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc tgccgcctcg
    15961 cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact tacggggttt
    16021 ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt agcgcaaaaa
    16081 attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga taccgatctc
    16141 agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg cgaaatggac
    16201 tggatggcac gtcacggtat gctgcgcgct cgcccccatg agttattgcc cggtacgctg
    16261 cgcgtgatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc cagcacgctg
    16321 aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta tcacaaactt
    16381 ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt ttcgctgaat
    16441 tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga aaaagctggg
    16501 ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc gttcttcttt
    16561 ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga ggaaggatgc
    16621 ggcaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga gccatatacc
    16681 gtcgatgctc gccgctgtat ctcttatctc accatcgaac tggaaggggc gatcccggaa
    16741 gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca gcttatctgc
    16801 ccgtggaatc gctattcaca actcaccaca gaagaggatt tcagcccgcg taagccgcta
    16861 cacgcaccgg aactcattga gttattcgcc tggagcgaag agaagttttt aaaagtcacg
    16921 gaaggatcgg cgattcgtcg tattggtcac ctgcgttggc tgcgtaatat cgccgtagca
    16981 ttaggcaatg cgccctggga tgaaacgatt ctggcggcgc tggagagtcg caaaggtgag
    17041 cacccacttc ttgatgagca catagcgtgg gcgattgcgc agcaaatcga gagacgaaat
    17101 gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt aattgaaaaa
    17161 gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa aacgcattgc
    17221 aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc ttaattttta
    17281 acttatctat caataggtta aaaaaatact attcaccgtg cgaagtttct ttgaaattaa
    17341 gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag acaacaaaaa
    17401 ccagcaataa cgcatcgcgt cgctgtggat attttattga gagaagaatt tggagcggga
    17461 aacgagactc gaactcgcga ccccgacctt ggcaaggtcg tgctctacca actgagctat
    17521 tcccgctttg gtggtctgtg ccttacagca ctttcaaatt ttggagcggg aaacgagact
    17581 cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta ttcccgcttg
    17641 ggtggtctgt gctttgcagc acttttcaaa ttttggagcg ggaaacgaga ctcgaactcg
    17701 cgaccccgac cttggcaagg tcgtgctcta ccaactgagc tattcccgcg tactacttaa
    17761 ttttgctttc gtaattttcg catttctgcg tcgttacggg aggcgcatta tacgagaaat
    17821 ctttccctct gcaacccccc taaaaaattt tttttgagtt tttatctcaa gtgctgaaat
    17881 tatcagcaag gcgttcaatt tagcgtcaaa atcacgccgc cgcaagtgga ttccgacgct
    17941 tacgtgacca ggaaaaattt tacagcttga taaaatgctc gcggtagtaa gccagctccg
    18001 ccaccgattc acggatatca tccatcgcct gatgcgtccc ctgcttggta aaaccatcca
    18061 gaatttccgg cttccagcgg cgcgccagct ctttcagggt gctgacatcg agataacggt
    18121 agtggaagta ggcttccagc tccggcatgt atttaaacag gaagcgacga tcctggccga
    18181 tgctgttacc gcaaatcggc gattttcccg caggcaccca ctgttttaag aattcgagcg
    18241 ttgccagttc agcttcacga tcgcccatcg tgctcgcttt cacgcgctct accagcccgc
    18301 tggcggtatg ggtgcgcacg ttccagtcat ccatcagcgc cagctgttca tcggactggt
    18361 gtactgcaat ggtcggccct tctgccagaa tattcaggtt ggcatcggtc accagcgtgg
    18421 caatctcaat aatgcgatcg cgctcgggat ccagaccggt catctcaaga tcgatccaaa
    18481 tcaggttgtt ttcattggca ctcatgctat tttccaccca ttcacagtga ctatattgat
    18541 ccacaattgc gtgtatcata gatgttttgc ccatcagggg cgaccaggag tctgtacgat
    18601 tgagtaaaaa taaactctcc aaaggccagc agcgccgcgt gaacgccaat caccagcgtc
    18661 gtcttaaaac gtctaaggag aagcccgact acgacgacaa tctgtttggc gagcctgatg
    18721 aaggtatcgt catcagccgc tttggtatgc acgctgatgt ggaatccgcc gatggcgacg
    18781 ttcaccgctg caatattcgc cgtaccatcc gttcgctggt gaccggcgac cgcgtagtct
    18841 ggcgtccggg taaaccggcg gcggaaggcg taaatgtcaa agggatcgtg gaagcggtgc
    18901 atgagcgcac ctcggtgttg acgcgtccgg atttctacga cggcgtgaaa cctattgccg
    18961 ccaacatcga ccagattgtc attgtctccg ccattttgcc ggagctgtca ctcaatatta
    19021 tcgaccgtta cctggtggcc tgcgaaacct tgcagattga gccgattatt gtgctcaaca
    19081 agatagacct gctggacgac gaaggcatgg cgttcgtcaa cgaacagatg gatatctacc
    19141 gcaatatcgg ttatcgcgta ttgatggttt ccagccatac tcaggatggg ctaaaaccgc
    19201 tggaagaggc gttgaccggg cgcatcagca tttttgccgg gcagtctggc gtcggcaaat
    19261 ccagcctgct gaacgcgttg ctggggctgc aaaaagagat cctgaccaac gatgtctctg
    19321 acaactcggg gcttggtcag cacaccacga ccgccgctcg gctgtatcac ttcccgcacg
    19381 gcggtgatgt gattgattcc cctggtgtgc gtgagttcgg cctctggcac ctggagccgg
    19441 aacaaatcac tcagggcttt gtcgaattcc atgactattt aggtctgtgt aaatatcgcg
    19501 attgcaaaca cgataccgat ccgggctgcg ctatccggga agcggttgag gaagggaaaa
    19561 tcgcggaaac ccgtttcgaa aactatcacc gtattctgga aagcatggcg caggtaaaaa
    19621 cgcgtaaaaa cttttctgat acggatgact gacaattaag ctaaccgtcg ttagaatcgt
    19681 cccctttttt tcaggatccg gcatgtaggc cggatcagga acgacaaaac aatggcctgg
    19741 aggctacctt gttaaattca tttaaacttt cgctacagta cattctgccg aaactatggc
    19801 ttactcgcct ggcgggttgg ggcgcaagca agcgggcagg atggctgaca aaactggtta
    19861 tcgatctgtt cgttaaatac tacaaggtcg acatgaaaga ggcgcaaaag ccggacaccg
    19921 ccagctaccg cacctttaac gaattctttg tccgtccgct gcgtgacgaa gtacgcccaa
    19981 tcgataccga tccgaatgtc ctggtcatgc ctgccgatgg cgttatcagc cagttgggta
    20041 aaatcgaaga agataaaatc ctgcaagcca aaggccacaa ctacagcctc gaagccctgc
    20101 tggcaggcaa ctatctgatg gcggacctgt tccgcaacgg tacgtttgtg accacttacc
    20161 tctccccgcg tgactaccac cgcgtacaca tgccgtgcaa cggtattctg cgtgagatga
    20221 tctacgtgcc gggcgatctc ttctccgtta accatctcac ggctcagaac gtgccgaatc
    20281 tgtttgcccg taacgaacgc gtgatctgcc tgttcgatac cgaatttggc ccaatggcgc
    20341 agattctggt cggtgcgacg attgtaggca gcattgagac tgtctgggcg ggcaccatta
    20401 cgccgccgcg cgaaggcatc atcaagcgct ggacctggcc tgccggggaa aacgacggtt
    20461 ctgtggcgct gctgaaaggc caggaaatgg gtcgctttaa acttggctct accgttatca
    20521 acctgtttgc accgggtaaa gtgaatctgg ttgagcaact ggaaagcctg tctgtcacga
    20581 aaattggcca gccgctggca gtatctaccg aaacctttgt tacgccagac gctgaaccag
    20641 ccccacttcc tgctgaagag atcgaagcag aacacgacgc cagcccattg gttgacgata
    20701 aaaaagacca ggtctaatcc atcaaaggaa acgctgacgt gcgcctgatt atcacttttc
    20761 tgatggcctg gtgcctcagt tggggggcgt acgccgcgac ggcccccgat agcaaacaaa
    20821 tcacccagga actggagcag gcaaaagcgg cgaaacccgc gcagccagaa gtcatagagg
    20881 cactccagtc tgccttaaat gcgcttgagg aacgaaaagg ttcccttgag cgcatcaagc
    20941 aatatcaaga agttattgat aattatccga aactctccgc tactctgcgc gcacaattaa
    21001 acaacatgcg tgacgagccg cgcagcgtgt cgccggggat gtctaccgac gcgctgaatc
    21061 aggaaattct ccaggtcagc agtcagttgc tggataaaag ccgtcaggcc cagcaagagc
    21121 aggagcgcgc ccgcgagatt gccgattcgc tgaatcaact gccgcaacag caaaccgacg
    21181 cccgccgtca gttaaacgag atcgagcgcc gcctgggaac gcttaccggc aatactccgc
    21241 tcaatcaggc acaaaatttc gcgttgcagt ctgactctgc acgtcttaag gcgctcgttg
    21301 atgaactgga gctggcgcag ctgtctgcca ataaccgcca ggaattagcg cgcttgcgct
    21361 ctgagctggc tgaaaaagag agccagcaac tggatgcgta tttgcaggcc ttgcgtaatc
    21421 aattgaacag ccaacgccag cttgaggcag agcgggcgct ggaaagtacc gaattactag
    21481 cagaaaacag tgccgatttg ccgaaagata tcgtcgcgca attcaaaatt aaccgcgaac
    21541 tatcggcagc gctgaatcaa caggcgcagc ggatggatct cgttgcctcg caacagcgtc
    21601 aggctgccag ccagacgtta caggtccggc aggcgttgaa tacgctgcgt gaacagtcgc
    21661 aatggctggg atcgtccaat ctgctcggtg aagcgttgcg ggcgcaggtg gcacggctgc
    21721 cggaaatgcc gaaaccacaa cagcttgata ccgaaatggc gcagttgcgt gtgcaacggt
    21781 tacgttatga ggatctgctt aataaacagc cgctgctacg gcaaattcat caggccgacg
    21841 gtcagccgct gaccgccgag caaaaccgta ttctggaagc acaactacgc actcagcgtg
    21901 agttgctgaa ctcattgttg cagggtggcg acacactact gctggaactg accaagctga
    21961 aagtctctaa cgggcaactg gaggatgcgc tgaaagaggt gaacgaagcg acgcaccgct
    22021 atctgttctg gacctctgac gtgcgcccga tgaccatcgc ctggccgctg gaaatcgccc
    22081 aggatctgcg tcgtctcatt tcgctggaca ccttcagtca gttgggcaaa gccagtgtga
    22141 tgatgctgac cagcaaagag acgattttgc cgctgtttgg cgcgttgatt ctggtcggtt
    22201 gcagtattta ctcgcgccgc tattttaccc gttttctcga acgttcggcg gcgaaagtcg
    22261 gcaaagtgac tcaggatcac ttctggctga cgttacgcac tcttttctgg tcgattctcg
    22321 tcgcgtcacc gctgccggtg ctgtggatga cgctgggtta cggcttgcgc gaggcgtggc
    22381 cttatccgct ggcggtcgcg attggcgatg gtgtaacggc caccgtgccg ctgctgtggg
    22441 tagtgatgat ttgcgccacc tttgcccgcc cgaacggctt gtttatcgct cattttggct
    22501 ggccgcgcga acgtgtttcc cgtgggatgc gctactacct gatgagcatc gggcttattg
    22561 tgccactgat tatggcgctg atgatgttcg ataacctcga cgaccgcgaa ttttccggtt
    22621 cgctgggacg gctttgcttt atcctcattt gcggtgcgct ggcggtggtc accctcagcc
    22681 tgaaaaaggc cgggataccg ctgtatctca acaaagaggg cagcggcgac aatattacca
    22741 accatatgtt gtggaacatg atgattggcg cgccactggt tgccattctg gcgtcggcgg
    22801 tgggttatct ggcaacggca caggcgctgt tagcgaggct tgaaacctcg gttgccatct
    22861 ggttcctgct actggtggtt tatcatgtta tccgccgctg gatgctgatc cagcgccgca
    22921 ggctggcgtt tgaccgggcg aagcatcgcc gggcagagat gttagcgcaa cgtgcacgtg
    22981 gcgaagagga agcgcatcat cacagtagcc cggaaggagc aattgaagtc gatgaaagcg
    23041 aagtcgatct cgatgccatt agtgcgcaat ccttgcggct ggtgcgctca attttgatgt
    23101 tgatcgccct gctttcggtc attgtgctgt ggtcagaaat ccattccgct ttcggcttcc
    23161 tcgaaaatat ttcgctgtgg gatgtcactt ccacggtaca gggcgtggaa agtctggagc
    23221 cgattaccct cggtgcgggg ctgattgcca ttctggtgtt tatcatcacc acgcagctgg
    23281 tgcgcaatct gcccgcgctg ctggaactgg cgattttgca gcacctggat ttaacgccgg
    23341 gtaccggcta cgccatcacc accatcacca aatatctgct gatgctgatt ggcgggctgg
    23401 tcggcttctc aatgattggt attgagtggt cgaaattgca gtggctggtc gccgcgctcg
    23461 gtgttggtct cggttttggt ttgcaggaaa ttttcgccaa ctttatctct ggcctgatca
    23521 tcctgttcga aaaaccgatt cgcattggcg atacggtaac gatccgcgat cttactggta
    23581 gcgtgacaaa aattaacacc cgcgccacca ccatcagcga ctgggaccgt aaagagataa
    23641 tcgtgccgaa caaggcgttt attaccgagc agtttatcaa ctggtcgctc tctgactcgg
    23701 tcacgcgcgt ggtgttgacg attccggccc ctgccgatgc caacagtgaa gaagtgacgg
    23761 aaatcctgct caccgcagcg cgtcgctgct cgctggtgat cgacaacccg gcaccggaag
    23821 tcttcctggt ggatctgcaa caggggattc agattttcga gctgcgtatt tacgccgctg
    23881 agatgggtca ccgtatgccg ctacgccatg agatccacca gctgattctg gctggcttcc
    23941 atgcccacgg tatcgatatg ccattcccgc ccttccagat gcgtctggaa agcctcaacg
    24001 gtaaacaaac ggggagaacg ctgacgtctg cgggcaaagg tcgtcaggcg ggaagtttgt
    24061 aatgcatgaa gctgccaaat gatgtcaacg tggctcattt agcagcttaa catcatcttc
    24121 agttatcacc ggccctggag agtatcatcc atacgcatta cttcctgaca actcatcgcc
    24181 ggagagacga tgagttgcag gatttcatat gatcagtcga gagctaactc aggtttattc
    24241 gccatgcgac tgcaatagtt ctggtaaatc gccatcgcta acagcgagaa gaagacaggg
    24301 ccgccaatca tccacaatgt gctgtcccag tctccggctt caaccacagg ttgaatgatg
    24361 gtgaagacgt tcgcaaatgt caccaccagt acgacaacca ctgtcgcaat cattgccgac
    24421 atacgcgttt tgaaaatcac aaatggtctg tcgagatcct gacgtgcttt aaagaatggg
    24481 aaagccagcg cgaggaacag gtaaggaagc gtcatagaca cgttcgccat cagcgtcagc
    24541 ttgttaaaga acgccgatgc ggtaccgcca ccaaacgaaa ccagcaggat gaagacagta
    24601 accaacccgc actgcatcca catggcgata gacggcatcc ccatcgcatt caggcgcgtc
    24661 atcggttccg gccacaacgc tttcggcgtc ccctggatga tggctttcag cggtgaatag
    24721 caaagcgtaa agaacgcacc ggtataagcg aggaacatcg aaagtccggt aatacgcgca
    24781 aaccatacgc ccagcgacaa tgacgcttca ggtgacaaat gcagtgcgtt acccagcgtc
    24841 accccgaggc tcttcatcag cacataggta atattgccaa ggttaacgga accattactt
    24901 aatacctgct gccagtttgt gctgacgccc cataaaaata ttgccagcga ataaccgatt
    24961 gaaataacaa tagcggcaaa aacaatacct ttggcaaagt tcttttctgg attttccgtt
    25021 ttatcgacca gaccaccaac cgcttcaatt ccgccatagg caaaaatagc aaataccaca
    25081 aacgatagca ttgccagacc ggactgataa cctgggttcg gtgatgcaag gaaattaata
    25141 tcctgcgcga aatgcccacc atttaataac aaaatagtaa tgcttactaa cagcaatact
    25201 aaattcagac acattactgc aatacccccc accgcagtaa tgcgggcaat tttattaatc
    25261 ccctttgaag caacgacggt gaccagaatc atccatgcca ctgccagcag accaaccacc
    25321 tgcgtaggct ccagtccggc aatacgccag tgctgggtca tgtcgctacc atagaggaat
    25381 gttgagaacg gtacccaaac tttcgcggag gtgctcacca tccagatgat ataagaggaa
    25441 aaccacataa acgtaccaat gaaggcaaaa cgtggtccga cactattatt catccaggaa
    25501 tagataccgc cttcttcttt gcgataagca gctcccattt cagccatcat taaggcgaat
    25561 ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag
    25621 taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt
    25681 cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt
    25741 gtataaaacg cgcccactga actgcactgc ccaacattga gcgggcagcg taaaaaccaa
    25801 tacagaattg atacctggat gatatcaaat atccagtctt aaattgtggc ctttagcgga
    25861 aaaatgaaaa catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag
    25921 taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg
    25981 cacccagcgc caacatcacc agacgatcaa cgcctaatgc cacgccggaa cagtcaggca
    26041 taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg
    26101 ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgtcaatt
    26161 catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc
    26221 gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa
    26281 aggtcggttt ttctttacca atatttggtt ctacgccaaa ggtaaacagc aattgtagca
    26341 acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgccactt
    26401 cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt
    26461 gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca
    26521 cctcgttcat caaccggtac atatcatagt gcggtcgata ccactcaagc atagtgaact
    26581 cagggttgtg ataacgcccc atctcttcat tacggaaact gcggcacagc tggaataccg
    26641 gcccgcaacc ggcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga
    26701 gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa
    26761 tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg
    26821 caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg
    26881 cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca
    26941 cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaataaa acaacggtgg
    27001 gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga
    27061 taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc
    27121 agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc
    27181 aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca
    27241 aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat
    27301 tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat
    27361 gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag
    27421 cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt
    27481 cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtgggggtg
    27541 cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat
    27601 cgagcgtacc tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca
    27661 gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct
    27721 ggttgatgat ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt
    27781 gcagatccgt gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg tttatcgtta
    27841 caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt
    27901 tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat
    27961 cctgatgacc gaaggttgcc gtggcgaagg cggtattctg gtcaacaaaa atggctaccg
    28021 ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata
    28081 tatggaactg ggtccacgcg acaaagtctc tcaggccttc tggcacgaat ggcgtaaagg
    28141 caacaccatc tccacgccgc gtggcgatgt ggtttatctc gacctgcgtc acctcggcga
    28201 gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt
    28261 cgatccggtt aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat
    28321 cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc
    28381 ctctgttggg ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt
    28441 cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa
    28501 cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa
    28561 ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg ctatggaaga
    28621 aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca agctggctga
    28681 gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga
    28741 cctgctctac accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc
    28801 cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga
    28861 gcgtgacgac gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac
    28921 tcgcctggag tacagcgacg tgaagattac tacgctaccg ccagctaaac gcgtttacgg
    28981 tggcgaagcg gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg
    29041 ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac
    29101 cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg
    29161 gctacatcaa agacaacctg gcaccggacc tgagctaccg ctggtcctgc cgtatggcga
    29221 tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc
    29281 tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg
    29341 atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca
    29401 tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcga
    29461 agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt
    29521 ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg
    29581 aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg
    29641 tatggagctg tactttcgtg ggctactgct ccgaagtctg cccgaaacac gtcgatccgg
    29701 ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga
    29761 aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg
    29821 tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg
    29881 gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc
    29941 ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttattgt gatcattaac
    30001 ctgatcactc tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa
    30061 gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc
    30121 tgggcggtaa ctgtagttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga
    30181 gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt
    30241 cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggttggtat
    30301 tctgctgccg ctggggctgt ttccgggtga tgcactgagc tacgagcgcg ttctggcgtt
    30361 cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg
    30421 tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtac ctgcgggcaa
    30481 atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac
    30541 aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa
    30601 ccagacccta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca
    30661 tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag
    30721 caacaaaaga tcccgggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt
    30781 acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag
    30841 ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg
    30901 gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg
    30961 aacgggatca cgctattaca tctcgcaacc tacaccgctg gcggcctgcc attgcaggtg
    31021 ccggatgagg tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca
    31081 tgggcgccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg
    31141 gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca
    31201 ctcaaactca accatacgtg gattaatgta ccgcccgcag aagaaaagaa ttacgcctgg
    31261 ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaaacttat
    31321 ggtgtgaagt cgaccattga agatatggcc tgctgggtac gaagcaatat gaatccccgt
    31381 gatatcaacg acaaaacact tcagcaaggg atacaactgg cacaatctcg ctactggcaa
    31441 accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt aaatcctgac
    31501 agcatcatta acggcagtga caataaaatt gcactggcag cacgccccgt aaaagcgatt
    31561 acgcctccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga
    31621 tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac
    31681 aaaaactatc ccaatccagc gagagtcgcc gccgcctggc agattctcaa cgctctgcag
    31741 taaaatttca tcgggtccga attttcggac cttttctccg cttttccttg ctgtcatcta
    31801 cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc
    31861 gacagctgca tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtgaccgt
    31921 agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga
    31981 tcaccgcttt gagcgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg
    32041 cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga
    32101 agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg
    32161 tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt
    32221 ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga
    32281 agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaaatttat
    32341 ttgggtacag cagcccggca gttagtgagt gctgagtttc agaccaataa tccccaatac
    32401 gattaacgcc agactcgcca ggcgcatcgg gttagcggac tcaccgagca gcacaatgcc
    32461 ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctactgg
    32521 taacgatttc atcgcccagg caagtagcgc catactgaca atcatcgccg tcacggtaat
    32581 aacactcggc gtcaaacgac taaagccgtg ggtatatttc aggccaacgg cccataccac
    32641 ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg
    32701 gccgtccccg gtgaaaagac gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga
    32761 tattttgcca ggtggcaggg gaatttcaac tattaccttt gtgactatcg cagagaaaac
    32821 aaacggcacg acacagctgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcac
    32881 cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg
    32941 cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca
    33001 taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca
    33061 acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc
    33121 agcgcgggag atggagttgc cgagatgttt gatgtcttcg ccgaaaccgc gagcggtgtt
    33181 acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc
    33241 tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg
    33301 agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag
    33361 cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt
    33421 atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt
    33481 aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca
    33541 tttcgcgttg tcgccaattg cttttgcgtc agcagacagc tgctcgaaag tttcgttgtt
    33601 catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc
    33661 agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag
    33721 acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc
    33781 ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct
    33841 atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg
    33901 cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg
    33961 ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt
    34021 tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc
    34081 gcatggagaa aggcaatccg gacgatccgc ttttgcgtca ggtaattacc tcgcaagatg
    34141 agtttgccat cgcgcccgga ttctccaccg acccgctgga agaacagcac agcgtagtgc
    34201 ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg
    34261 taaattgccg ttattgcttc cgtcgccact tcccttatgc agaaaatcag ggcaacaagc
    34321 gtaactggca aactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt
    34381 tctccggcgg cgatccgctg atggcgaaag atcacgagct ggactggttg ctcacacaac
    34441 tggaagccat cccgcatatc aaacgtctgc ggattcacag tcgtctgccg attgtgatcc
    34501 cggcacgcat cacagatgcg ctggttgaac gcttttcaca ttctacgctg caaatcttgc
    34561 tggtgaatca catcaaccat gccaatgaga tagatgaaac attccgtcag gcgatggcta
    34621 agttgcgccg ggtaggcgtt actttgctga accagagcgt tctgttacgt gatgtgaacg
    34681 ataacgcaca aacgctggca aacctgagta atgcgttgtt cgatgccggc gtgatgccgt
    34741 attacctgca tgtgctcgat aaagtacagg gtgcggcgca ttttatggtg agtgatgacg
    34801 aagcacggca gattatgcgt gagttgctga cactggtgtc gggatatctg gtgccgaaac
    34861 tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc
    34921 agtaactgat taaacaggga agtcaaacat taaaattgcc tgatgcgcta cgcttatcag
    34981 gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt
    35041 ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat taaacaggga
    35101 agtttaatct ttgcccgttg cataaccttt aattattcat atgcattgct gttgttactc
    35161 tttatataac taacaccaat aattaacct
//
LOCUS       DAZKFU020000014        35127 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZKFU020000014 DAZKFU020000000
VERSION     DAZKFU020000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.014"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..637
                     /locus_tag="ACHOQQ_RS02850"
                     /old_locus_tag="pgaptmp_000570"
     CDS             <1..637
                     /locus_tag="ACHOQQ_RS02850"
                     /old_locus_tag="pgaptmp_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HGU4468872.1"
                     /translation="KNEINKEDILKLMAAREKVAGASDKWTKASGLYSAIVKGHTEIV
                     AAWMETAEVIASHYENDKDVVRELLSLSRNNAVCSLYVASYKTMSKQVIDVYLNAAIR
                     LALQHGFTFDEIVEQFTRDFDGKSFSHVVEKADDIYMGLWLKIFKIVVGENENYLKDV
                     MMQLEAKNNEGKSVISQANGNPVFKELFWKAIDEFNFPQEELNRLNQYRSL"
     gene            complement(776..2140)
                     /gene="mnmE"
                     /locus_tag="ACHOQQ_RS02855"
                     /old_locus_tag="pgaptmp_000571"
     CDS             complement(776..2140)
                     /gene="mnmE"
                     /locus_tag="ACHOQQ_RS02855"
                     /old_locus_tag="pgaptmp_000571"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGU4468873.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGISEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2246..3892)
                     /gene="yidC"
                     /locus_tag="ACHOQQ_RS02860"
                     /old_locus_tag="pgaptmp_000572"
     CDS             complement(2246..3892)
                     /gene="yidC"
                     /locus_tag="ACHOQQ_RS02860"
                     /old_locus_tag="pgaptmp_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGU4468874.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3895..4152)
                     /gene="yidD"
                     /locus_tag="ACHOQQ_RS02865"
                     /old_locus_tag="pgaptmp_000573"
     CDS             complement(3895..4152)
                     /gene="yidD"
                     /locus_tag="ACHOQQ_RS02865"
                     /old_locus_tag="pgaptmp_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGU4468875.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(4116..4475)
                     /gene="rnpA"
                     /locus_tag="ACHOQQ_RS02870"
                     /old_locus_tag="pgaptmp_000574"
     CDS             complement(4116..4475)
                     /gene="rnpA"
                     /locus_tag="ACHOQQ_RS02870"
                     /old_locus_tag="pgaptmp_000574"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGU4468876.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4492..4632)
                     /gene="rpmH"
                     /locus_tag="ACHOQQ_RS02875"
                     /old_locus_tag="pgaptmp_000575"
     CDS             complement(4492..4632)
                     /gene="rpmH"
                     /locus_tag="ACHOQQ_RS02875"
                     /old_locus_tag="pgaptmp_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGU4468877.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4862..4942)
                     /locus_tag="ACHOQQ_RS02880"
                     /old_locus_tag="pgaptmp_000576"
     CDS             complement(4862..4942)
                     /locus_tag="ACHOQQ_RS02880"
                     /old_locus_tag="pgaptmp_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4468878.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            5239..6642
                     /gene="dnaA"
                     /locus_tag="ACHOQQ_RS02885"
                     /old_locus_tag="pgaptmp_000577"
     CDS             5239..6642
                     /gene="dnaA"
                     /locus_tag="ACHOQQ_RS02885"
                     /old_locus_tag="pgaptmp_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGU4468879.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6647..7747
                     /gene="dnaN"
                     /locus_tag="ACHOQQ_RS02890"
                     /old_locus_tag="pgaptmp_000578"
     CDS             6647..7747
                     /gene="dnaN"
                     /locus_tag="ACHOQQ_RS02890"
                     /old_locus_tag="pgaptmp_000578"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGU4468880.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7747..8820
                     /gene="recF"
                     /locus_tag="ACHOQQ_RS02895"
                     /old_locus_tag="pgaptmp_000579"
     CDS             7747..8820
                     /gene="recF"
                     /locus_tag="ACHOQQ_RS02895"
                     /old_locus_tag="pgaptmp_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGU4468881.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8849..11263
                     /gene="gyrB"
                     /locus_tag="ACHOQQ_RS02900"
                     /old_locus_tag="pgaptmp_000580"
     CDS             8849..11263
                     /gene="gyrB"
                     /locus_tag="ACHOQQ_RS02900"
                     /old_locus_tag="pgaptmp_000580"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGU4468882.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11287..11385)
                     /locus_tag="ACHOQQ_RS02905"
                     /old_locus_tag="pgaptmp_000581"
                     /pseudo
     CDS             complement(11287..11385)
                     /locus_tag="ACHOQQ_RS02905"
                     /old_locus_tag="pgaptmp_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11503..11901
                     /gene="yidB"
                     /locus_tag="ACHOQQ_RS02910"
                     /old_locus_tag="pgaptmp_000582"
     CDS             11503..11901
                     /gene="yidB"
                     /locus_tag="ACHOQQ_RS02910"
                     /old_locus_tag="pgaptmp_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGU4468883.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGDVAPQANNDLLSAGMELLKGKLFR"
     gene            12016..12828
                     /gene="yidA"
                     /locus_tag="ACHOQQ_RS02915"
                     /old_locus_tag="pgaptmp_000583"
     CDS             12016..12828
                     /gene="yidA"
                     /locus_tag="ACHOQQ_RS02915"
                     /old_locus_tag="pgaptmp_000583"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGU4468884.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12874..13530)
                     /gene="yidX"
                     /locus_tag="ACHOQQ_RS02920"
                     /old_locus_tag="pgaptmp_000584"
     CDS             complement(12874..13530)
                     /gene="yidX"
                     /locus_tag="ACHOQQ_RS02920"
                     /old_locus_tag="pgaptmp_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGU4468885.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGTSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13666..13779
                     /locus_tag="ACHOQQ_RS02925"
                     /old_locus_tag="pgaptmp_000585"
                     /pseudo
     CDS             13666..13779
                     /locus_tag="ACHOQQ_RS02925"
                     /old_locus_tag="pgaptmp_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13776..14840)
                     /gene="cbrA"
                     /locus_tag="ACHOQQ_RS02930"
                     /old_locus_tag="pgaptmp_000586"
     CDS             complement(13776..14840)
                     /gene="cbrA"
                     /locus_tag="ACHOQQ_RS02930"
                     /old_locus_tag="pgaptmp_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGU4468886.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDNKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFATLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFICGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            14941..16155
                     /gene="yidR"
                     /locus_tag="ACHOQQ_RS02935"
                     /old_locus_tag="pgaptmp_000587"
     CDS             14941..16155
                     /gene="yidR"
                     /locus_tag="ACHOQQ_RS02935"
                     /old_locus_tag="pgaptmp_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGU4468887.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGEMVSFTYNDHVMHERSPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCALVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPKDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAESGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16157..16489)
                     /gene="yidQ"
                     /locus_tag="ACHOQQ_RS02940"
                     /old_locus_tag="pgaptmp_000588"
     CDS             complement(16157..16489)
                     /gene="yidQ"
                     /locus_tag="ACHOQQ_RS02940"
                     /old_locus_tag="pgaptmp_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4468888.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASASM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16729..16801
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16795..17208
                     /gene="ibpA"
                     /locus_tag="ACHOQQ_RS02945"
                     /old_locus_tag="pgaptmp_000589"
     CDS             16795..17208
                     /gene="ibpA"
                     /locus_tag="ACHOQQ_RS02945"
                     /old_locus_tag="pgaptmp_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGU4468889.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17320..17748
                     /gene="ibpB"
                     /locus_tag="ACHOQQ_RS02950"
                     /old_locus_tag="pgaptmp_000590"
     CDS             17320..17748
                     /gene="ibpB"
                     /locus_tag="ACHOQQ_RS02950"
                     /old_locus_tag="pgaptmp_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGU4468890.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            17944..19605
                     /gene="yidE"
                     /locus_tag="ACHOQQ_RS02955"
                     /old_locus_tag="pgaptmp_000591"
     CDS             17944..19605
                     /gene="yidE"
                     /locus_tag="ACHOQQ_RS02955"
                     /old_locus_tag="pgaptmp_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGU4468891.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19602..20318)
                     /gene="yidP"
                     /locus_tag="ACHOQQ_RS02960"
                     /old_locus_tag="pgaptmp_000592"
     CDS             complement(19602..20318)
                     /gene="yidP"
                     /locus_tag="ACHOQQ_RS02960"
                     /old_locus_tag="pgaptmp_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4468892.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            20614..22230
                     /locus_tag="ACHOQQ_RS02965"
                     /old_locus_tag="pgaptmp_000593"
     CDS             20614..22230
                     /locus_tag="ACHOQQ_RS02965"
                     /old_locus_tag="pgaptmp_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4468893.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22230..23552
                     /locus_tag="ACHOQQ_RS02970"
                     /old_locus_tag="pgaptmp_000594"
     CDS             22230..23552
                     /locus_tag="ACHOQQ_RS02970"
                     /old_locus_tag="pgaptmp_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGU4468894.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23549..24442)
                     /gene="yidL"
                     /locus_tag="ACHOQQ_RS02975"
                     /old_locus_tag="pgaptmp_000595"
     CDS             complement(23549..24442)
                     /gene="yidL"
                     /locus_tag="ACHOQQ_RS02975"
                     /old_locus_tag="pgaptmp_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4468895.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24609..26324
                     /gene="yidK"
                     /locus_tag="ACHOQQ_RS02980"
                     /old_locus_tag="pgaptmp_000596"
     CDS             24609..26324
                     /gene="yidK"
                     /locus_tag="ACHOQQ_RS02980"
                     /old_locus_tag="pgaptmp_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGU4468896.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26321..27814
                     /gene="yidJ"
                     /locus_tag="ACHOQQ_RS02985"
                     /old_locus_tag="pgaptmp_000597"
     CDS             26321..27814
                     /gene="yidJ"
                     /locus_tag="ACHOQQ_RS02985"
                     /old_locus_tag="pgaptmp_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4468897.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARTEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYQLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVNTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEDKKQKF"
     gene            complement(27861..28310)
                     /gene="yidI"
                     /locus_tag="ACHOQQ_RS02990"
                     /old_locus_tag="pgaptmp_000598"
     CDS             complement(27861..28310)
                     /gene="yidI"
                     /locus_tag="ACHOQQ_RS02990"
                     /old_locus_tag="pgaptmp_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGU4468898.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28419..28766
                     /gene="yidH"
                     /locus_tag="ACHOQQ_RS02995"
                     /old_locus_tag="pgaptmp_000599"
     CDS             28419..28766
                     /gene="yidH"
                     /locus_tag="ACHOQQ_RS02995"
                     /old_locus_tag="pgaptmp_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGU4468899.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28756..29118
                     /gene="yidG"
                     /locus_tag="ACHOQQ_RS03000"
                     /old_locus_tag="pgaptmp_000600"
     CDS             28756..29118
                     /gene="yidG"
                     /locus_tag="ACHOQQ_RS03000"
                     /old_locus_tag="pgaptmp_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGU4468900.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29115..29612
                     /gene="yidF"
                     /locus_tag="ACHOQQ_RS03005"
                     /old_locus_tag="pgaptmp_000601"
     CDS             29115..29612
                     /gene="yidF"
                     /locus_tag="ACHOQQ_RS03005"
                     /old_locus_tag="pgaptmp_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU4468901.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDTLQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     HPRRDCQACDWHRLCPHAREETPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29620..30804)
                     /gene="emrD"
                     /locus_tag="ACHOQQ_RS03010"
                     /old_locus_tag="pgaptmp_000602"
     CDS             complement(29620..30804)
                     /gene="emrD"
                     /locus_tag="ACHOQQ_RS03010"
                     /old_locus_tag="pgaptmp_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGU4468902.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNGGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            30886..30960
                     /locus_tag="ACHOQQ_RS03015"
                     /old_locus_tag="pgaptmp_000603"
     CDS             30886..30960
                     /locus_tag="ACHOQQ_RS03015"
                     /old_locus_tag="pgaptmp_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4468903.1"
                     /translation="MNVSQICARNGEKKNGRICKQKRQ"
     gene            complement(31084..31173)
                     /gene="tisB"
                     /locus_tag="ACHOQQ_RS03020"
                     /old_locus_tag="pgaptmp_000604"
     CDS             complement(31084..31173)
                     /gene="tisB"
                     /locus_tag="ACHOQQ_RS03020"
                     /old_locus_tag="pgaptmp_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGU4468904.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31738..31836
                     /gene="ivbL"
                     /locus_tag="ACHOQQ_RS03025"
                     /old_locus_tag="pgaptmp_000605"
     CDS             31738..31836
                     /gene="ivbL"
                     /locus_tag="ACHOQQ_RS03025"
                     /old_locus_tag="pgaptmp_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGU4468905.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            31942..33630
                     /gene="ilvB"
                     /locus_tag="ACHOQQ_RS03030"
                     /old_locus_tag="pgaptmp_000606"
     CDS             31942..33630
                     /gene="ilvB"
                     /locus_tag="ACHOQQ_RS03030"
                     /old_locus_tag="pgaptmp_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGU4468906.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPVRVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33634..33924
                     /gene="ilvN"
                     /locus_tag="ACHOQQ_RS03035"
                     /old_locus_tag="pgaptmp_000607"
     CDS             33634..33924
                     /gene="ilvN"
                     /locus_tag="ACHOQQ_RS03035"
                     /old_locus_tag="pgaptmp_000607"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4468907.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34187..>35127
                     /locus_tag="ACHOQQ_RS03040"
                     /old_locus_tag="pgaptmp_000608"
     CDS             34187..>35127
                     /locus_tag="ACHOQQ_RS03040"
                     /old_locus_tag="pgaptmp_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4468908.1"
                     /translation="MASPINSGIMMSNLCSSTFLDKNINVAAELDITNSEKKYSPGSN
                     FAKWMLQEIKRLIANIMSGSRSVNTEILNYFHPMPGTENNGNRTWMAATGENEYTVIE
                     QTGGKSFKVIQFDAGEKNTKKIVKETSYSGVAVATIIKSLSEKTAALETHSADTVLRK
                     KLVNSIVMKNTDFKHVITADIENNIYDLLKLRVQQDEGNLPVQKDPEFPDDCFDSMRA
                     DAHRCVKDQEQKKSGTKINSGTKITCCGVELSKDILKILADKTKKYETAVEHTLGLVV
                     ADVCFKEDECSQVRELLKKPNGNVTDDKIDEIVACLA"
BASE COUNT         8558 a         8821 c         9262 g         8486 t
ORIGIN      
        1 taaaaatgaa ataaataaag aagatatatt gaaactgatg gcagcaagag aaaaagtggc
       61 gggagcatca gacaaatgga cgaaggcatc aggcttatat tctgcgatag tgaaagggca
      121 tacggagatt gttgctgcct ggatggagac agctgaagtg atagccagcc attatgaaaa
      181 tgataaagat gtagtgagag aactactgtc gctgagcaga aataatgcag tttgctcttt
      241 gtatgttgcc agctataaga caatgagtaa gcaggtcatt gatgtatatc tgaatgcggc
      301 gattcgcctg gcgttgcaac acgggttcac tttcgatgaa attgtggagc agtttacccg
      361 tgactttgat gggaagtcat tctctcatgt ggtagagaaa gcggatgata tatatatggg
      421 actctggctg aaaatattca aaattgtggt tggtgaaaat gaaaattatc ttaaagatgt
      481 gatgatgcaa ctggaagcga agaataatga aggtaaatct gtaataagtc aggcaaatgg
      541 caatcctgta tttaaagaac tattctggaa agcaatagac gaatttaact ttccgcaaga
      601 ggagcttaat cggttaaacc aatatcgttc tctgtaaaga caggactgca agatgttgaa
      661 agtcgctcca gccagtagta aaggcagttt tggtgcagga tgctcataaa aaaacaagcg
      721 ctgttccgtt ctgatagtag aacggaacag tgccctttat atcaattaag acgacttact
      781 taccaataca gaagctggag aaaatccgcc ccagcaggtc gtcagaggta aattccccgg
      841 tgatttcgct taagttctgc tgcgccaggc gcaactcttc cgccagtagt tcgcctgccc
      901 aggcacctaa cagttgcgct ttaccttgct gcaaatgctc cgctgcctgt tccagcgcct
      961 gtaggtggcg acggcgcgcc aggaagccgc cttccatgtt ggtgtcaaag cccatgctct
     1021 gtttgagatg gttacgcagc acgtccacgc cttcgccagt ccttgcagac agacgaatta
     1081 acgcgtgacc gttcacttca cttattccca gcgtttcgcc ggtgatatcg gctttattgc
     1141 gcaccacggt gatcggcagt ttcgctggca gacgggcgat aaattccggc cagatctctg
     1201 ccggatcaac ggcgtctgtc gtggtgccat cgaccataaa cagcacgcgg tcggcctgtt
     1261 caatttcctg ccacgcgcgc tcgatcccaa tacgttcaac ttcgtcactg gcttcacgta
     1321 gcccggcggt atcgatgata tgcagcggca ttccgtcaat atggatatgc tcacgcagca
     1381 cgtcacgcgt ggttccggcg atatcggtta cgattgccgc ttcacggccc gccagcgcgt
     1441 ttaacaggct cgatttaccg gcgttaggac gtccggcaat caccaccttc atcccttcgc
     1501 gcaacaaact accctgacgt gcttcagcac gcactgcatc gagatcggca atcacgtcat
     1561 taagctgggc ttcaattttt ccgtcggaga ggaaatcgat ctcttcatcg gggaaatcaa
     1621 ttgccgcttc gacgtaaatg cgcaagtggg tgagggcttc caccagatgg ttgacccgtg
     1681 cggagaatgc gccttgcagc gagttaagtg ccgaacgggc tgcctgttcc gaactggcat
     1741 cgataagatc ggcaatcgcc tcggcctggg ctaagtcgag tttatcgtta agaaacgcgc
     1801 gttcggaaaa ctcaccaggg cgagcaatcc gcaggccggg aatggtcaga atgcgtttta
     1861 acagcaggtc gaggatcacc ggaccgccat gaccctgcag ttccagcaca tcttcgccgg
     1921 tgaacgagtt cgggccaggg aaccatagcg caatcccctg atcgagcacg ctgccgtcgg
     1981 cgtctttaaa cggaagataa tcggcgtagc gcggcttagg cagtttaccc agcacggttt
     2041 cggcaacttc acgggctttg aagccggaga tgcgcaggat gccaacgccg ccacgtcccg
     2101 gaggcgtggc ctgggctacg atagtgtcat tatcgctcat gatgttcctg ttgctttgtg
     2161 tggcggatgc gcggtgctta tccgccctac gaaaagaaaa aaggcggtca actgaccgcc
     2221 cttattttag cgaaaactca ccgaatcagg attttttctt ctcgcggcta tgcaggccac
     2281 gtttttccag accacggtaa atcagctgct gctgaataat ggttaccagg ttgctgacga
     2341 tatagtacag caccagacct gacgggaacc acaggaagaa cacggtgaag atgaccggca
     2401 taaaggtcat gatcttctgc tgcatcgggt cggtcacagt ggtcggcgac atcttctgaa
     2461 tgaagaacat cgttacgccc atcaggatcg gcaggatgta gtacgggtcc tgtgcagaca
     2521 ggtcgtggat ccacagtgcg aacggcgcct gacgcagttc aacggaaccc atcagcatgt
     2581 agtacaacgc caggaagatt ggcatctgga tcagcagcgg gaagcagccg cccagcgggt
     2641 taaccttctc agctttgtac agcgccatca tttcctggct gatacgctgt ttgtcatcgc
     2701 ccagacgctc acgcatcgcc tgaatcttcg gctgcagcat acgcatcttc gccatggagg
     2761 tgtactgcgc tttggtcagc gggtacatga tgccacgaac gataaaggtg atgatgataa
     2821 tggagaagcc ccagttaccc acaaagctat ggatccattt cagcagtttg aacagcggct
     2881 gagagatgaa ccacaaccaa ccgtaatcaa cggtcagatc caggtgcgga gcaacagctg
     2941 ccattttgtc ctggatttcc gggccaaccc acagggtgct gttcatcgcg ccagtctgac
     3001 caggctgaac cagtaccggc tgagatttat agccgatagc ggcgatgccg ttacccagat
     3061 tagcagtata gaagttgttg gtaccgtcgt tatgcgggat ccacgccgtc gcgaaatact
     3121 gttgcagcat tgccacccaa ccacctttcg aagagatgtt caggttttcg ttatcggcaa
     3181 tggtatcgaa cttgtatttc tcatacttct cgtcaggcgt ggagtacgcc gcgccacgga
     3241 aggtgtgcag tgcgaagttg ctgcttccgg tatcgagatg cggtggcaga gtgatggatt
     3301 gcttcaactg accaaatgag gagatttcca gcggtttctc gccagcattc tgcacgttgt
     3361 agttgacgtt gacagcgtaa tcaccacgtt ttaggacaaa cgttttggta aacgtgttgc
     3421 ctgccgcgtc ggtatacgtc atcggcacct gcagttcgtt ttgaccttca gccagcacat
     3481 aagcgtcttt ttcaacgtta tacagcggac gcgggccgtt agccgggtta tccgggccat
     3541 cacgaccggt cagaccgctc tgtgcctgat aaataaactg cggtgaagtt tccagcagct
     3601 ggaacggctg ggtagagttc agctctttcg ggtaagcagg cagcagagct tgctcaacat
     3661 caccaccacg ggtgttgatg gtcagatcaa gcacgtcggt cttaaccgag atcagtttcc
     3721 cctggccact ggccggtacg ccctggtcgg cggcgctacc cgctgcggtg gtcgttgtct
     3781 gcgtggtctg ttgggcctga ggttgcgggt ttttatcctg ctcccaggct tgccagatca
     3841 tgaaagacac gaacagcaaa gcgatgacta aaagattgcg ttgcgaatcc atcgttagtg
     3901 ttctctggta tcaaatggtc cgggcgggac gggatcgtca ccaccagggt gtaaagggtg
     3961 gcattttaat acgcgtttca ccgtcaacca actgcctttt atcactccaa acctgcgcaa
     4021 tgcctcaatt ccgtagcttg aacaggttgg agtgaaacga caatgcggcc cgagtagcgg
     4081 actaatcagg cgttgataga cccgaatgag ggctatcagg acccgcgagc caggcgacag
     4141 tggcggcgcc ataatttttc caacgcttcc gagagagcac ggttatcgag gtcggcaacc
     4201 ccttttttcg ccaccaccac gaaatccata gccgggagtt catgttggcg cagacggaag
     4261 ctttcacgcg tcagacgttt aatccgattg cgttcatggg cgcgtcgaac gtttttcttg
     4321 gcgactgtaa gaccgatacg gggatgcccc agcgaattca ggcggccgag aatggtaatt
     4381 tgcggcgtgc cagcccgttg tggctgctgg aagacgaatg tgaattgact gggagttaac
     4441 aagcgtaact ccctgggaaa tgcgagctta accactcagg ggttagcttt attacttaga
     4501 aacggtcaga cgagcgcggc ctttagcacg acgacgtgcc agaacctgac gaccattttt
     4561 agtagccata cgagcacgga agccgtgaga acggttgcgc ttcagtacag acggttgaaa
     4621 agtgcgtttc atggcgattt ctacctaaac ttgaataaat tcaatggctt tattggatat
     4681 ccgccgaaaa atgaaacgat ggacaccgaa gccatgggtg attaaagagg ccggattgta
     4741 ataattgtac actccggagt caattctctt tccttattta ccgcgctttt ccgcaccttt
     4801 tcgcagggaa aacgtacggc ctcacaccag tgaaaaccag catggcgcgc cgggtggagg
     4861 attatacggg ctgacgggta aagcgcaagg atcgtcctgg atctttatta gatcgattaa
     4921 gccaattttt gtctatggtc attaaatttt ccaatatgcg gcgtaaatcg tgcccgcctc
     4981 gcggcaggat cgtttacact tagcgagttc tggatcatcc tgtggataaa tcgggaaaat
     5041 ctgtgagaaa cagaagatct cttgcgcagt ttaggctatg atccgcggtc ccgatcgttt
     5101 tgcaggatct tgatcgggca tataaccgca gacagcggtt cgtgcgtcac cctcaagcag
     5161 ggtcttttcg acgtacgtca acaatcatga atgtttcagc cttagtcatt atcgactttt
     5221 gttcgagtgg agtccgccgt gtcactttcg ctttggcagc agtgtcttgc ccgattgcag
     5281 gatgagttac cagccacaga attcagtatg tggatacgcc cattgcaggc ggaactgagc
     5341 gataacacgc tggccctgta cgcgccaaac cgttttgtcc tcgattgggt acgggacaag
     5401 taccttaata atatcaatgg actgctaacc agtttctgcg gagcggatgc cccacagctg
     5461 cgttttgaag tcggcaccaa accggtgacg caaacgccac aagcggcagt gacgagcaac
     5521 gtcgcggccc ctgcacaggt ggcgcaaacg cagccgcaac gtgctgcgcc ttctacgcgc
     5581 tcaggttggg ataacgtccc ggccccggca gaaccgacct atcgttctaa cgtaaacgtc
     5641 aaacacacgt ttgataactt cgttgaaggt aaatctaacc aactggcgcg cgcggcggct
     5701 cgccaggtgg cagataaccc tggcggtgcc tataacccgt tgttccttta tggcggcacg
     5761 ggtctgggta aaactcacct gctgcatgcg gtgggtaacg gcattatggc gcgcaagccg
     5821 aatgccaaag tggtttatat gcactccgag cgctttgttc aggacatggt taaagccctg
     5881 caaaacaacg cgatcgaaga gtttaaacgc tactaccgtt ccgtagatgc actgctgatc
     5941 gacgatattc agttttttgc taataaagaa cgatctcagg aagagttttt ccacaccttc
     6001 aacgccctgc tggaaggtaa tcaacagatc attctcacct cggatcgcta tccgaaagag
     6061 atcaacggcg ttgaggatcg tttgaaatcc cgcttcggct gggggctgac tgtggcgatc
     6121 gaaccgccag agttagaaac ccgcgtggcg atcctgatga aaaaggccga cgaaaatgac
     6181 attcgtttgc caggcgaagt ggcgttcttt atcgccaagc gtctacgatc taacgtacgt
     6241 gagctggaag gcgcgctgaa ccgcgtcatt gccaacgcca actttaccgg acgggcgatc
     6301 actatcgact tcgtgcgtga ggcgctgcgc gacttgctgg cattgcagga aaaactggtc
     6361 accatcgaca atattcagaa gacggtggcg gagtactaca agatcaaagt cgcggatctc
     6421 ctttccaagc gtcgatcccg ctcggtggct cgtccgcgtc agatggcgat ggcgctggcg
     6481 aaagagctga ctaaccacag tctgccagag attggcgatg cgtttggtgg tcgtgaccac
     6541 actacggtgc ttcatgcctg ccggaagatc gagcagctgc gtgaagagag ccacgatatc
     6601 aaagaagatt tttcaaattt aatcagaaca ttgtcatcgt aaacctatga aatttaccgt
     6661 agaacgtgag catttattaa aaccgctaca acaggtgagc ggtccgttag gtggtcgtcc
     6721 tacgctaccg attctcggta atctgctgtt acaggttgct gacggtacgt tgtcgctgac
     6781 cggtactgat ctcgagatgg aaatggtggc acgcgttgcg ctggttcagc cacacgagcc
     6841 aggagcgacg accgttccgg cgcgcaaatt ctttgatatc tgccgtggtc tgcctgaagg
     6901 cgcggaaatt gccgtgcagc tggaaggtga gcggatgctg gtgcgctccg ggcgtagccg
     6961 tttttcgctg tctaccctgc cagcggcgga tttcccgaac ctcgatgact ggcagagtga
     7021 agtcgaattt accctgccgc aggcgacgat gaagcgtctg attgaagcga cccagttttc
     7081 tatggcgcat caggacgttc gctattactt aaacggtatg ctgtttgaaa ccgaaggtga
     7141 agaactgcgc accgtggcga ccgacggcca ccgtctggcg gtctgttcaa tgccaattgg
     7201 tcaatctttg ccaagccatt cggtgatcgt gccgcgtaaa ggcgtgattg aactgatgcg
     7261 tatgctcgac ggcggcgata atccgctgcg tgtgcagatt ggcagcaaca atattcgcgc
     7321 ccacgttggc gactttatct tcacctctaa actggtggat ggtcgcttcc cggattaccg
     7381 ccgcgttctg ccgaagaatc cggacaaaca tctggaagca ggctgcgatc tcctcaagca
     7441 ggcgtttgct cgcgcggcaa ttctctctaa cgagaaattc cgcggcgtgc gtctgtatgt
     7501 cagcgaaaac cagctgaaaa tcaccgccaa taacccggaa caggaagaag cggaagagat
     7561 cctcgacgtt acctatagcg gtgcggagat ggaaatcggc ttcaacgtca gctatgtgct
     7621 ggatgtcctg aacgcgctga aatgtgaaaa cgtccgcatg atgctgaccg attcggtttc
     7681 cagtgtgcag attgaagatg ccgcatcaca gtcggctgcc tatgttgtca tgccaatgag
     7741 actgtaatgt ccctcacccg cttgttgatc cgcgatttcc gcaacattga aaccgcggat
     7801 ctcgccttat ctcccggctt taactttctg gtaggtgcca acggcagtgg caaaaccagc
     7861 gtgctggaag ccatctatac gctcggccat ggtcgggcgt ttcgcagttt gcagattggt
     7921 cgcgtcattc gccatgagca ggaggcgttt gttctccacg ggcgattaca gggcgaagag
     7981 cgcgagacgg cgattggctt aaccaaagac aaacagggcg acagcaaagt ccgcatcgac
     8041 ggtactgacg ggcataaagt cgcggaactg gcgcacctga tgccaatgca gctgataacg
     8101 ccagaagggt ttactttact caacggcggc cccaaataca gaagagcatt cctcgactgg
     8161 ggatgctttc acaacgaacc cggatttttc accgcctgga gcaatctcaa gcgattgctc
     8221 aagcagcgca atgcggcgct gcgccaggtg acacgttacg aacagctacg cccgtgggat
     8281 aaagaactga tcccgctggc agagcaaatc agcacctggc gcgcggagta tagcgccggt
     8341 atcgcggccg atatggctga tacctgtaag caatttctcc ctgagttttc tctgactttc
     8401 tctttccagc gcggctggga gaaagagaca gaatatgctg aggtgctgga acgtaatttt
     8461 gaacgcgatc gccagctaac ctacaccgcg catggcccgc ataaagcgga cttacgcatt
     8521 cgcgccgacg gtgcgccggt ggaagatacc ttatcgcgtg ggcagcttaa gctgttgatg
     8581 tgcgccttac gtctggcgca aggagagttc ctcacccgtg aaagcgggcg gcggtgtctc
     8641 tacctgatag atgattttgc ctctgagctt gatgatgagc gtcgcgggct gcttgccagc
     8701 cgcttaaaag cgacgcaatc gcaggtcttt gtcagcgcga tcagtgctga acacgttata
     8761 gacatgtcgg acgaaaattc gaagatgttt accgtggaaa agggtaaaat aacggattaa
     8821 cccaagtata aatgagcgag aaacgttgat gtcgaattct tatgactcct ccagtatcaa
     8881 agtcctgaaa gggctggatg cggtgcgtaa gcgcccgggt atgtatatcg gcgacacgga
     8941 tgacggcacc ggtctgcacc acatggtatt cgaggtggta gataacgcta tcgacgaagc
     9001 gctcgcgggt cactgtaaag aaattatcgt caccattcac gccgataact ctgtctccgt
     9061 acaggatgat gggcgcggca ttccgaccgg tattcacccg gaagagggcg tatcggcggc
     9121 ggaagtgatc atgaccgttc tgcacgcagg cggtaaattt gacgataact cctataaagt
     9181 gtccggcggt ctgcacggcg ttggtgtttc ggtagtaaac gccctgtcgc aaaaactgga
     9241 gctggttatt cagcgcgagg gtaaaattca ccgtcagatc tacgaacacg gtgtaccgca
     9301 ggctccgctg gcggttaccg gcgagactga aaaaaccggc accatggtgc gtttctggcc
     9361 cagcctcgaa accttcacca atgtgaccga gttcgaatat gaaattctgg cgaaacgtct
     9421 gcgtgagttg tcgttcctca actccggcgt ttccattcgt ctgcgcgaca agcgtgacgg
     9481 caaagaagac cacttccact atgaaggcgg catcaaggcg ttcgttgaat atctgaacaa
     9541 gaacaaaacg ccgatccacc cgaatatctt ttacttctcc accgaaaaag acggtattgg
     9601 cgtcgaagtg gcgttgcagt ggaacgatgg cttccaggaa aacatctact gctttaccaa
     9661 caacattccg cagcgtgacg gcggtactca cctggcaggc ttccgtgcgg cgatgacccg
     9721 taccctgaat gcctacatgg acaaagaagg ctacagcaaa aaagccaaag tcagcgccac
     9781 cggtgacgat gcgcgtgaag gcctgattgc ggtcgtttcc gtaaaagtgc cggacccgaa
     9841 attctcctcc cagaccaaag acaaactggt ttcttctgag gtgaaatcgg cggttgaaca
     9901 gcagatgaac gaactgctgg cggaatacct gctggaaaac ccaactgatg caaaaatcgt
     9961 ggttggcaaa attatcgatg ctgcccgtgc ccgtgaagct gcgcgtcgcg cgcgtgaaat
    10021 gacccgccgt aaaggtgcgc tcgacttagc gggcctgccg ggcaaactgg cagactgcca
    10081 ggagcgcgat ccggcgcttt ccgaactgta ccttgtggaa ggggactccg cgggcggctc
    10141 tgcgaagcag gggcgtaacc gcaagaacca ggcgatcctg ccgctgaaag gtaaaatcct
    10201 taacgttgag aaggcgcgct tcgacaagat gctctcttct caggaagtgg cgacgcttat
    10261 caccgcgctt ggttgtggta tcggtcgtga cgagtacaac ccggacaaac tgcgttatca
    10321 cagcatcatc atcatgaccg atgcggacgt cgacggctcg cacattcgta cgctgctgtt
    10381 gaccttcttc tatcgtcaga tgccggagat cgttgaacgc ggtcacgtct acatcgctca
    10441 gccgccgctg tacaaagtga agaaaggcaa gcaggaacag tacattaaag acgacgaagc
    10501 gatggatcag taccagatct ctatcgcgct ggacggcgca acgctgcaca ccaacgccag
    10561 cgcaccggca ttggctggcg aagcgttaga gaaactggtg tctgagtaca acgcgacgca
    10621 gaaaatgatc aaccgcatgg agcgtcgtta tccgaaagca atgctgaaag agcttatcta
    10681 tcagccgacg ctgacggaag ccgacctctc tgatgagcag accgttaccc gctgggtgaa
    10741 cgcgctggtc agcgaactga acgacaaaga acagcacggc agccagtgga agtttgatgt
    10801 ccacaccaat gccgaacaaa acctgtttga accgattgtt cgcgtgcgta cccacggtgt
    10861 ggatactgac tatccgctgg atcacgagtt tatcaccggt ggcgaatatc gtcgtatctg
    10921 cacgctgggt gagaaactgc gtggcttgct ggaagaagat gcgtttatcg aacgtggcga
    10981 gcgtcgtcag ccggtagcca gctttgagca ggcgctggac tggctggtga aagagtctcg
    11041 tcgcggtctc tccatccagc gttataaagg tctgggcgag atgaacccgg aacagctgtg
    11101 ggaaaccacc atggacccgg aaagccgtcg tatgctgcgc gttaccgtta aagatgcgat
    11161 tgctgccgac cagttgttca ccacgctgat gggcgacgcc gttgaaccgc gccgtgcgtt
    11221 tattgaagag aacgccctga aagcggcgaa tattgatatt taatggcgtt aaccatgcga
    11281 gcgtgcctga tgcgctacgc ttatcaagcc tacgagactc ctgcaatata ttgattttgc
    11341 atgtttttgc aggccggata aggcgttcac gccgcatccg gcaagaagct ataagaaaag
    11401 ggcggagatc atctccgccc tttttatttc tgcaatccgc aattatcatc ccgcagttct
    11461 atactttttg ggtcaacacg taacgcttta caggtgatta tcatggggct ttttgatgaa
    11521 gttgtcggcg cctttctgaa aggcgatgcg gggaaatatc aggcgatttt aagttgggtt
    11581 gaggagcagg gcggcattca ggtgctgctg gaaaaactgc aaagtggcgg cttaggggcc
    11641 attctctcaa cctggctgag taatcaacag ggcaatcaat cggtgagcgg cgaacaactg
    11701 gaatcggcgc tcggcacaaa tgcggtatcc gatctcgggc aaaaacttgg cgtggatacc
    11761 agtacggctt ccagtttact ggcagaacaa ttgccgaaga ttattgatgc tctctcgcca
    11821 cagggcgatg tagcgccgca agccaacaat gatttacttt ccgcagggat ggaactactg
    11881 aaagggaaac tcttccgcta agcaaatggg gagcatgcaa cccgcgttgc atcccccttt
    11941 tccctcgggt gttgtactga tttttgagcg gaatcgcgtt agcatgggtc aggaaccaat
    12001 ctacctgggg aactcatggc tatcaaactc attgctatcg atatggatgg cacccttctg
    12061 ctgcccgatc acaccatttc acctgccgtt aaaaatgcga ttgccgcagc tcgcgcccgt
    12121 ggcgtgaatg tcgtgctaac gacgggtcgc ccttatgcag gtgttcacaa ttacctgaaa
    12181 gagctgcata tggaacagcc gggtgactac tgcattactt ataacggcgc gctggtacag
    12241 aaggccgctg atggtagcac cgtggcgcaa actgctctca gctatgacga ctaccgtttc
    12301 ctggaaaaac tctctcgcga agtcggttct catttccacg cactggaccg caccacgctg
    12361 tacaccgcca accgtgatat cagctactac acggtgcatg aatcctttgt tgccactatt
    12421 ccgctggtgt tctgcgaagc ggagaaaatg gaccccaata cccagttcct gaaagtgatg
    12481 atgattgatg aacctgccat cctcgaccag gctatcgcgc gtattccgca ggaagtgaaa
    12541 gagaaatata ccgtgctgaa aagtgcgccg tacttcctcg aaatcctcga taaacgcgtt
    12601 aacaaaggta cgggtgtgaa atcactggcc gacgtgttag gtattaaacc ggaagaaatc
    12661 atggcgattg gcgatcagga aaacgacatt gcaatgattg aatatgcagg cgtcggtgtg
    12721 gcgatggata acgctattcc ttcggtgaaa gaagtggcga actttgtcac caaatctaac
    12781 cttgaagatg gcgtggcgtt tgctattgag aagtatgtgc tgaattaatc agtggacggg
    12841 caaacagcat aaacgcgttt gcccgcttac tgattattgg tttactgcat atacaaccag
    12901 aaattgcagc ggtgcagtaa tgatgtccag cgtaacagtc actggcagta atgctgcgga
    12961 aacaccatcc ggaatgcctc tgggtgaata gtattcataa aatcgcactt taaatggatg
    13021 gtagaacgcc atcacctttg agtaatccat gtttttattt ttctcatgga ttgtgccttt
    13081 taggttttcc agtgacctgg tacagtgttg aacaccacag gcaaaaccat aatgcgttgc
    13141 cagtgccttt tcttcttcgt tattccagta gtagtaaagc gatattgtgc cggtaaattt
    13201 ctttttcccc gcatttaaaa caaatcttgc gtcatcagca acctgaatat tgtgccggtt
    13261 aagtgctgga tcattgagca tcttaacgat gtcatcagca ccatctgtga taaggtaatc
    13321 cagcgattcc cctacaaaaa catagccttt tgttccatta ctatcctttg cctgcgatag
    13381 ccccgtgatg gtgtctgact gcgattgata ctttcctgaa gtaccctttg cggtatggga
    13441 aaccagagca tacgagatac agcctgaaag catcagtgct agtgggaata aactggcagc
    13501 cttaaaaaat cccttaaaat tcaacttcat gattaatcct ttaaacgagt agctaagccg
    13561 ttgaaagcat actcttcttt ccctcctgtt tagcgcctga tctccctttt cagatttctg
    13621 cccgatataa gaccttttct tatgaggtgt ttttttgtgg actaatatct gttgcaaggc
    13681 tatggcttcg ccccggcact ggtttatatc tccaccgtcg cgttgattgg tgcgctctct
    13741 tacatcctgc tggtgggcga tgtgaagcgc gttggctaat ccttcaactg tggaatatgt
    13801 gccacaccac tgcgcataat ccactttctt aaagccggtg cggtcaggca tcggcttttt
    13861 actatcttgc cgaagagttt taaacgcagt ttgcgggtgg cgcgccagta ggctgcgttg
    13921 atcttctctg gctgcttcag taacaccgaa cgcagaatct ctgcgctatc cagcgcatag
    13981 cttatcccct ccagcgagct ggcgctgata aatcccgccg cttcaccaat cagaaaggcg
    14041 ttgtccttac cgcagataaa atcctgccag cgtgagggaa acagcactgt gcatttttcg
    14101 cttttcaccg ctttgccaaa ctggaactga aaggcgctca ttttctcttt cagcgtcgcg
    14161 aaacgcgtct gaccgtcttt cattggatag gcaccaccaa agataaaata accgtctttg
    14221 ctgatactcc atgagtaaca gtcagttatc gcattatcaa agatgcagga gtagaacggc
    14281 accggatgtt tctctgcgaa ccactgctgg attgcgacat atttacggat ttgatgatcc
    14341 gggtagagat gtcgccgcac catcgagttg gcaccatcgg caccgaccag atagcgggcg
    14401 gtaatatgct gctcccagcc gtctgcacga aaaatgacat gccatttatt atcctcacgc
    14461 cagattttcc ggcacaggct atcgtggtaa acctcaacgc tggcggggat cagcgatttc
    14521 atccacaagt cgaaagcatg gcgattaata ttgatatagc ttcgctggta gttacgcgtg
    14581 agcgatgcgg cgacgtcgac ggttttgacg ctgaaaatct gcggattagc gatcacatcg
    14641 acaggaagcg tcagtccatc gcgaataaaa gatcgctgcg cgtccggtgc cagcagaccg
    14701 ccgcagggtt tgctgaaacc ttcagtacca cactggtgct ttttatccag cgcgatcacc
    14761 tgcattttgc ctgctaactt tcgtgccaac gccgatcctg ccgggccgag gccaataatc
    14821 gccacgtcga aatgttccat cttttcactc acattcatca cgatggcaaa agactattgt
    14881 ctgaaagtga agtccttgtg aagtatgatg gcgtgaaccg ttttgtatca ggaccacgcc
    14941 atgaaacaga taacctttgc tccccgtaat cacctgctca ccaataccaa tacctggacg
    15001 cccgacagcc agtggctggt atttgacgtg cgtccttcag gggcgtcgtt taccggcgag
    15061 accattgagc gtgtgaatat ccatactgga gaggtcgagg ttatctatcg cgcgtcacag
    15121 ggcgcacacg tcggcgtggt gaccgttcat ccgaagtcag aaaaatatgt cttcattcac
    15181 ggcccggaaa atcctgatga aacatggtat tacgatttcc atcatcggcg cggagtcatt
    15241 gctgaaggcg gcaaggtgag caatctcgat gcaatggata ttactgcacc gtacacccca
    15301 ggagcgctgc gcggcggcag ccatgtgcat gtctttagcc cgaacggtga aatggtgagc
    15361 tttacctata acgaccatgt aatgcatgaa cgcagcccgg cgctggattt gcgaaacgtc
    15421 ggggttgctg caccgtttgg cccggtcaac gtacaaaagc agcatccgcg tgaatacagc
    15481 ggtagccact ggtgcgcact ggtgagtaaa acaacgccca cgccgcagcc tggcagcgat
    15541 gaaatcaatc gtgcttatga agaaggatgg gtaggaaatc acgcgctggc gtttattggc
    15601 gacacacttt cgccaaaggg cgagaaagta ccggagctgt ttattgtcga gttgccgaaa
    15661 gatgaagctg gctggaaagc agctggtgat gcgccgttaa gcggaacgga aacaaccctg
    15721 cccgcgccac cgcgtggcgt cgtgcagcga cgtttaacct ttacccacca tcgggcttat
    15781 ccggggttag tcaacgtccc gcgccactgg gtgcgctgta atccgcaggg tacacaaatc
    15841 gcgtttttaa tgcgagatga taacggcatt gtgcaactgt ggcttatctc gccacagggc
    15901 ggcgagccgc gccagttaac ccataacaaa acggatattc agtctgcatt taactggcat
    15961 ccatcaggag aatggttggg ctttgtgctg gataatcgaa ttgcttgtgc ccatgcggaa
    16021 agcggggagg ttgagtattt aaccgaaaac cacgccaatc caccttctgc ggacgccgtg
    16081 gtcttctcgc cggatggtca atggctggcg tggatggaag gcggtcagct gtggatcacc
    16141 gaaactgatc gctaaatcag ttatcaggca ttctggccgg tggaataacc gcattggtgg
    16201 cttgcgcgtt ggcttcgctt ttttcaacgc gtgagcgcac cgagctgtct ttgcggaaca
    16261 catcccacgg cagtaaaagt gtgtccatca cagcggtaaa cggcatatcg agaatcgcca
    16321 gcgacttagt gccccagttg gtttcatcgt cgccaatcat gctggcgctg gcgcgggtgc
    16381 cgggatacgt tccttctttc cccccggtat gggacatcac gctggagcat cctccgagta
    16441 atgtcattcc gctgcatatc atgaacgcta acaacacatt tcttatcata ataaattcat
    16501 ctgttgatcg tgggtgttgg cctgatgagt tatagcgatc ccttgctgaa aataacatca
    16561 tcattacgtc gcactgtggc ggctatcgca ctttaacgtt tcgtgctgcc ccctcagtct
    16621 atgcaataga ccataaactg caaaaaaaag tccgctgata aggcttgaaa agttcatttc
    16681 cagacccatt tttacatcgt agccgatgag gacgcgcctg atgggtgttc tggctacctg
    16741 acctgtccat tgtggaaggt cttacattct cgctgatttc aggagctatt gattatgcgt
    16801 aactttgatt tatccccgct ttaccgttct gctattggat ttgaccgttt gtttaaccac
    16861 ttagaaaaca accagagcca gagtaatggc ggctaccctc cgtataacgt tgaactggta
    16921 gacgaaaacc attaccgcat tgctatcgct gtggctggtt ttgctgagag cgaactggaa
    16981 attaccgccc aggataatct gctggtggtg aaaggtgctc acgccgacga acaaaaagag
    17041 cgcacctatc tgtaccaggg catcgctgaa cgcaactttg aacgcaaatt ccagttagct
    17101 gagaacattc atgttcgtgg cgctaacctg gtaaatggtt tgctgtatat cgatctcgaa
    17161 cgcgtgattc cggaagcgaa aaaaccgcgc cgtatcgaaa tcaactaatt ccctaaggcc
    17221 gccttgcgcg gcctgacatc tccatgctcg ccgtcaggga gcatatgcga atcttcggat
    17281 ttgcaggtac ttactcgctt cttagaagga gaaatgacta tgcgtaactt cgatttatcc
    17341 ccactgatgc gtcaatggat cggttttgac aaactggcca acgcactgca aaacgccggt
    17401 gaaagccaga gcttcccgcc gtacaacatt gagaaaagcg acgataacca ctaccgcatt
    17461 acccttgcgc tggcaggttt ccgtcaggaa gatttagaga ttcaactgga aggtacgcgc
    17521 ctgagcgtaa aaggcacgcc ggagcagcca aaagaagaga aaaaatggct gcatcaaggg
    17581 cttatgaatc agccatttag cctgagcttt acgctggctg aaaatatgga agtctctggc
    17641 gcaaccttcg taaacggttt actgcatatt gatttaattc gtaatgagcc tgaacccatc
    17701 gcagcgcagc gtatcgctat cagcgaacgt cccgcgttaa atagctaact agctattctc
    17761 tttgccccgc cattctggtg gggctttttt tgtctttctt cttctacaat gcgttaaatt
    17821 tcgactgttt aagatatttc ggcacgttct ttcccgtgat aatgtgcgct ataaggcaaa
    17881 tctgcttcac cgattcagcc ataatccgta gcaattaaaa tgttatctca agagaaatgg
    17941 acgatgagtg atatagcatt aacggtcagt attctggctt tggtggcagt cgtcggtttg
    18001 tttatcggca acgtcaaatt tcgcggcata ggattaggta ttggcggcgt gctgtttggt
    18061 gggatcatcg tcggccattt tgtttctcag gcggggatga cattaagtag cgatatgctg
    18121 catgttattc aggaatttgg tctgatcctg ttcgtttata ctatcgggat tcaggtaggg
    18181 ccgggcttct ttgcctcatt gcgcgtctcc ggattacgcc tcaacctgtt tgctgttctg
    18241 atcgtcatca tcggtggtct ggttaccgcc atcctgcata aactgtttga tattccactg
    18301 ccggtagtgc tggggatttt ctccggtgcg gtaaccaata cgccagcgct gggggcaggg
    18361 cagcagatct tgcgcgacct gggtacacca atggaaatgg tcgatcagat ggggatgagt
    18421 tacgcgatgg cgtatccatt cggcatttgc gggatattgt tcaccatgtg gatgttgcgg
    18481 gttattttcc gcgtcaatgt cgagacagaa gcccagcagc acgaatcttc acgcaccaat
    18541 ggcggcgcgc tgatcaggac tatcaatatt cgcgttgaga accctaacct gcatgattta
    18601 gccattaaag atgtaccgat tctcaacggc gacaaaatta tctgctcgcg tctgaaacgc
    18661 gaagaaaccc taaaagttcc ttcgccagat accattatcc aactgggcga tttgctgcat
    18721 ctggtgggtc agccagcgga tttacataat gcgcaactgg tgattggtca ggaggtcgat
    18781 acttcgctgt ccacgaaagg cactgatttg cgcgtcgagc gtgtggtggt caccaatgaa
    18841 aacgtgcttg gtaagcgtat tcgcgacctg cattttaaag aacgctatga cgttgttatc
    18901 tcgcgcctga accgtgccgg ggtcgaactg gtcgccagtg gcgatatcag cctgcagttc
    18961 ggcgatatcc tcaacctggt ggggcgtccg tccgcaattg atgccgttgc caatgtgctg
    19021 gggaatgcgc agcaaaaact gcaacaggtt cagatgctgc cagtgtttat tggcatcggg
    19081 cttggcgtat tgttaggttc tattcccgtc tttgtgcctg ggttcccggc cgcgttgaaa
    19141 ctggggctgg cgggcggtcc gctgattatg gcgttgatcc tcgggcgtat cggcagtatc
    19201 ggcaagctgt actggtttat gccgccaagt gccaacctcg cgctgcggga gctggggatc
    19261 gtgctgttcc tctcggtcgt tggtctgaaa tctggtgggg attttgtgaa taccttggtc
    19321 aatggcgaag ggctaagctg gattggttat ggtgccctga tcaccgccgt tccgctgatt
    19381 actgttggca ttctggcgcg gatgttagcc aaaatgaatt acctgaccat gtgcgggatg
    19441 ctggcaggtt ccatgaccga tcctccggcg ctggcgtttg ctaataatct tcatccaacc
    19501 agcggtgcgg cggcgctctc ttacgccact gtctatccgt tagtgatgtt cctgcgcatt
    19561 atcacccccc aattactggc ggtgctcttc tggagtatcg gttaactctt gtccgggtgg
    19621 agtcgccgta aatggtactc cacctggtat tcgctggcat ttctgaacat cactgaataa
    19681 ttcaaaaact ccccgctctc gctatatgac aacgaggtga tccgcagcag tggcgtgtgt
    19741 tccgctacct tcatttgccg cgccagcagt ctatcggcga gcaccggcgt caggctttca
    19801 taattaccgc caatcagaat cccgcactct tgttcaatat attcaaactt cgacccttcc
    19861 agatgttgca gcgaaagatt acggaacagt tttaccggca tatagctgtc ttccagcatc
    19921 agcggtttcc cttccacaaa acgaacgcga cgggagaagt agatctgctc gttgatttga
    19981 atccgtaact ggctggcaat tgccggaggc gcaggcatga tttcaaaaat cagcacctgg
    20041 ctggtgactg tttttccctg ccgttttaac acctccacca gcccggtcag gctggcggtt
    20101 tgatgcagta catctttgcg caccaggtaa gtgccgctgc cgtggcggcg gaccaccagc
    20161 ccccacgcta ccagcaggtc aatggcttta cggatggtca tccgcgatac cgcaaactct
    20221 tccgccagct ttttttcacc gggaagaagg ctgtttagcg tgaaatctgc ggaattaagt
    20281 ctaattctta accgctccgc aatgcttttg tagatcatct ctagatctct tttttgcctg
    20341 caagcccggg taatgcgccc acaagttctg cttaatcgat tgaaaaaaca tctttttttt
    20401 aaagatgtgt tcgatgctgt gtacctgtgc tcacagaggt ctactttttc gcgaaaacgt
    20461 agatctctac cgcccaacga aaagcatgaa agcgatcacg aatcccattg ggtcgtcatg
    20521 ttctgccgat cgcatcttcc tatcctcgct ccaggcctgc cgcataacca atcaggcttc
    20581 ctacttacag aattgagaaa agaggatgtg gaaatgctca gtcaaattca acgctttggc
    20641 ggcgcgatgt tcacgccagt gctgctgttt cccttcgccg ggattgtggt gggtcttgcc
    20701 atcttgctgc aaaacccgat gtttgtcggg gaatcactga ccgatccgaa cagtttattc
    20761 gcgcaaatcg tacacattat tgaagagggc ggatggacgg tattccgtaa tatgccgctg
    20821 atttttgctg tcggtttacc cattggcctt gctaagcaag cgcaggggcg tgcttgtctg
    20881 gcggtgatgg tgagtttcct gacctggaac tatttcatca acgcgatggg aatgacctgg
    20941 ggaagctact tcggcgtcga tttcactcag gacgcggtgg caggtagcgg tctgacaatg
    21001 atggccggga ttaaaaccct cgataccagc attatcggcg caattatcat ttccggcatt
    21061 gtgacggcgc tgcataaccg tctgttcgat aaaaaactgc cggtgtttct cggcattttc
    21121 caggggacgt cttatgtggt gattatcgcc ttcctggtga tgatcccctg tgcctggctg
    21181 acattgctcg gctggccaaa agtacaaatg gggattgaat ctctgcaagc gttcctgcgt
    21241 tcggcgggtg cgcttggggt gtgggtttac accttcctcg aacgtattct gatcccaacc
    21301 ggtttacacc acttcatcta cggaccgttt atctttggtc cggcagctgt tgaaggcggc
    21361 attcagatgt actgggcgca gcatctgcaa gagttcagtt tgagcgccga gccgctgaaa
    21421 tcgttgttcc cggaaggcgg ttttgccctg cacggtaact caaaaatctt tggtgccgtg
    21481 ggcatttctt tagcgatgta cttcactgcc gcaccggaaa atcgggtaaa agtggcgggc
    21541 ttgctgattc ccgcaacctt aaccgccatg ctggtgggaa ttaccgaacc gctggaattt
    21601 accttcctgt tcatttcacc gttgctgttt gcggtacacg ctgtgcttgc ggcctcaatg
    21661 tcgaccgtga tgtatctctt tggtgtggtg ggcaacatgg gcggaggtct gattgaccag
    21721 gttttaccgc aaaactggat cccgatgttc agcaaccacg cggatatgat gttgacccaa
    21781 atcgccattg ggttgtgctt taccctgctg tacttcgtgg ttttccgcac cctgattctg
    21841 caattcaaca tgtgcacgcc gggacgtgaa gatgcggaag tgaaactcta ctcaaaagcc
    21901 gaatacaaag cctcgcgagg ccaaaccacc gcggcagagc caaaaaaaga gctggatcag
    21961 gctgccggta tcctgcaagc cctgggcggg gtcggcaata tctccagcat caacaattgc
    22021 gcgacgcgtc tacgtattgc actgcatgac atgtcacaaa cgctggatga cgaagtcttt
    22081 aaaaagctgg gagcgcacgg cgtcttccgt agtggcgatg ccattcaggt gatcattggt
    22141 ctgcatgtat cccagctgcg tgaacagctc gatagcttaa ttaattctca tcaatcagca
    22201 gaaaatgttg ccattacgga ggcagtataa tgaccaaatt ctcagtggtt gtcgcaggcg
    22261 gtggaagcac ctttacgcca ggcatcgtgt tgatgctcct ggcgaatcag gaccgtttcc
    22321 cgcttcgtgc actgaaattt tatgataacg atggcgcgcg gcaggaagtg attgccgaag
    22381 cctgtaaagt catccttaaa gagaaagcgc cggacattgc gtttagttac accaccgatc
    22441 ctgaagtggc gttcagcgac gttgattttg tcatggcgca catccgcgtc ggcaaatacc
    22501 cgatgcgcga actggatgaa aaaatcccgc tgcgccacgg cgttgttggt caggaaactt
    22561 gcggacccgg cggaatagcg tacggcatgc gttccattgg cggcgtcctg gaactggtgg
    22621 attatatgga aaaatattca ccaaacgcct ggatgctcaa ctactccaac ccggcagcca
    22681 ttgtcgcaga agccacgcgt cgtctgcgcc cgaatgcgaa aatcctcaac atctgtgaca
    22741 tgccaatcgg tattgaaagc cggatggcgc aaattgttgg gctgcaagat cgcaaacaga
    22801 tgcgcgtgcg ctactacggc ctgaaccact ttggctggtg gacatcaatt gaagatttgc
    22861 agggcaacga cctgatgccc cagctgcggc aatatgtctc gaagcacggt tatgttccac
    22921 cgcagcaaga tacacatact gaagcgagct ggaacgacac ctatgcaaaa gcgcgggatg
    22981 tccaggcact ggacccggat acattaccaa acacctatct gaaatattat ctcttcccgg
    23041 attatgtcgt tcagcattcc aaccctgaac atacccgcgc gaatgaggtg atggaacatc
    23101 gcgagaaaca ggttttcgat gcttgccgcg ccattacggc ggcaggaaat tcagcggcgg
    23161 gcaagctgga gattgacgaa catgcgtcat acatcgtcga tctggcggcg gcaattgcct
    23221 tcaacactca ggagcggatg ttgctgattg tgcctaacaa cggggcaatc cataactttg
    23281 atgatgaagc gatggtcgag atcccgtgtc tggtcgggca caacggacca gaaccactgg
    23341 tggtcggcga tatcccgcag tttcagaaag ggttaatgag tcagcaagtg gcggtggaaa
    23401 aactggtcgt ggacgcctgg gaacagcgtt catatcagca cctgtggcag gcgattacgt
    23461 tgtcgaaaac ggtaccgagc gcctcggtcg ccaaagctat tctggatgag ttgctggagg
    23521 ccaacaaagc gtactggcca gagttacgtt aattgtgtgg actggcatcc gttgggtgct
    23581 ggtccgtatt ctttagcacg gctgaaggcg cataaccaaa tttatgttta aaggctttgc
    23641 tgaaatgaaa ggagtcgaag aagttgagcg tatcagcgac ttcgccaacc gagtttcctt
    23701 gttgctttaa tagcgatagc gccagatcca gacgcgcatc gaggtaatat tctttcggcg
    23761 tcttgccggt atagcgtaaa aacagacgac gcaaccaggc ttcgctacag gggatcgtgg
    23821 cagccatatc ggctacgctc cagcgttgtt gcagactggc atgtaaagtg gcaattaatt
    23881 tttcaatttg ccgccgttgt ggatcttttt taccgtctgc gtacataaga caaatccact
    23941 gataaataat tttcgtcaga aaagcgactg ccagattatt ttttattgct tctggtgaag
    24001 ttattaactc agcaacttcg gtgagttcct gattataaat ttcgccatta taaataacgc
    24061 tttgctgacc aacgggaata tccatcatac tggtgggggt aaattccatc cagtactgtt
    24121 cccagactaa accttcacag tgataagagt gaatatccat tggctttaaa aatataatac
    24181 agttgccatg cagagttatt tgttcgccat tctttaaaag aattttccca catccttgta
    24241 cggtatacac cgcaacccat gagttggcga taagcggttt cttcttgtcc cgcggccaga
    24301 tcacccggta atgctcgtcg gcgtaggtat gccagagcga gatcagagaa attccgctgg
    24361 agatcacatt ttcgttaatc aataagggaa tattgtatgg agtttcgttt tttacatctg
    24421 aagtttgcaa ttttccattc attttaatat ccctccggcg aattatgctc acaacctgaa
    24481 cttaacagaa aacaatccgt tcagggatac gacgtttgta ttttaagaat ctgactgcct
    24541 gacccgacgc attttacctc tccctatatt catgcgtccg ggacataaac ataaataagg
    24601 gctatgagat gaattcgtta caaatcttga gttttgtcgg ttttacgctg ctggtggcga
    24661 tcatcacctg gtggaaggtt cgcaaaacag ataccggatc gcaacaaggc tattttcttg
    24721 ccggacgttc actaaaagcg ccggttattg ctgcttcgtt aatgttaacc aacctttcca
    24781 cggaacaact ggttggtctt tccgggcagg cctacaaaag cggcatgtcg gtgatgggct
    24841 gggaagtgac ttcagcggtg acgctgatct tcctcgcgct aatcttttta ccgcgctatc
    24901 tgaagcgcgg cattgccacc atccccgatt ttctggagga acgttatgat aaaacgacgc
    24961 gtattatcat cgacttctgc ttcctcattg ccaccggcgt ctgctttctg ccgattgttc
    25021 tctactccgg cgcgttggcg ctcaacagcc tgtttcacgt cggggaatcg ctacagattt
    25081 ctcacggtgc ggctatctgg ctactggtaa ttttgcttgg tctggcggga attttgtatg
    25141 cggtgatcgg cggactgcgc gcaatggcag tggcggactc catcaacggt attgggctgg
    25201 ttatcggcgg gttgatggtg ccggtatttg gcctgatcgc gatgggcaag ggcagcttta
    25261 tgcagggcat tgagcaaatt accaccgttc acgccgagaa attaaactca gtcggtggcc
    25321 cgaccgatcc cttgccgatt ggcgcggcat ttaccggttt aattctggtg aacacctttt
    25381 actggtgtac aaatcagggc atcgtgcaac gcacgctggc gtcaaaaagc ctggcggaag
    25441 ggcaaaaggg ggcgctgtta acggcggtgc tgaaaatgct cgacccgctg gtactggtgc
    25501 tgccagggtt gattgcgttt catctgtatc aggatctacc gaaagccgac atggcctacc
    25561 cgacgctggt caataacgtt ctgccagtgc cactggtggg tttcttcggc gcggtgttat
    25621 ttggtgcggt gatcagtacc ttcaacggct ttctgaatag cgccagtacg ttattcagta
    25681 tgggtattta ccgtcgcatc attaaccaga atgccgagcc gcagcagctg gtcaccgtcg
    25741 ggcgcaaatt tggtttcttt atcgctatcg tttcggtgct ggtagcgccg tggatcgcca
    25801 acgcgccgca ggggctgtat agctggatga aacagctcaa cggcatttac aacgtgccgc
    25861 tggttaccat catcattatg ggctttttct tcccgcgcat cccggcgctg gcggcaaaag
    25921 tggcgatggg gattggcata atcagctaca tcaccatcaa ctatctggtg aagttcgact
    25981 tccatttcct ctatgtgctg gcctgtacgt tctgcatcaa cgtggtcgtg atgctggtga
    26041 tcggttttat caaaccgcgc gccacgccgt tcaccttcaa agatgcgttt gcggtggaca
    26101 tgaaaccgtg gaaaaacgtc aagatcgcgt caattggcat cctgttcgcg atgattggcg
    26161 tctatgccgg gctggctgaa ttcggcggct acggtacgcg ctggttagcg atgatcagtt
    26221 atttcattgc cgccgtagtg attgtctacc tgatttttga cagctggcgg catcgtcacg
    26281 acccagccgt aacctttact cccgatgcaa aggatagcct atgaaacgcc ccaattttct
    26341 gttcatcatg accgataccc aggccactaa tatggtcggt tgctatagcg gtaagccgct
    26401 gaatacgcaa aatattgata gtctggcggc ggaaggtatt cgctttaact ccgcctacac
    26461 ctgttcaccg gtttgtacac cggcgcgcgc cgggctgttt accggtatct acgctaacca
    26521 gtccggcccg tggaccaaca acgtcgcgcc aggcaaaaac atttccacta tggggcgcta
    26581 ctttaaggat gcgggctatc acacttgtta catcggcaaa tggcatctcg acggtcatga
    26641 ctatttcggc actggcgagt gtccgccgga gtgggacgct gattactggt tcgatggggc
    26701 gaactatctt agcgaactga cggaaaaaga gattagcctg tggcgcaatg gcctaaacag
    26761 cgtcgaagat ttacaggcga accatattga cgaaaccttc acctgggcgc accgcatcag
    26821 caatcgggcg gtggattttc tgcaacagcc cgcgcgcacc gaggaaccct tcctgatggt
    26881 ggtttcgtat gatgagccgc atcacccgtt cacctgtccg gtggagtatt tagagaaata
    26941 cgctgatttt tactaccaac tgggcgagaa agcacaggat gacctggcga acaaaccaga
    27001 acatcaccgc ttatgggcgc aggcgatgcc atcgccagtc ggtgatgacg ggctttatca
    27061 ccatccgctc tattttgcct gtaatgactt tgttgatgac caaataggac gggtcatcaa
    27121 tgccttaacg ccagagcaac gtgaaaatac gtgggttatt tatacatccg atcacggcga
    27181 aatgatgggc gcacataagc tgatcagtaa aggagcggcg atgtatgacg acatcacccg
    27241 tattccgctg atcatccgtt cgccgcaagg ggagcggcga caggtcaata cgccagtcag
    27301 tcatatcgat ttactgccga caatgatggc gctggcagat attgaaaaac cagagattct
    27361 gccgggggaa aatatccttg ccgtgaaaga gccacgcggc gtgatggtgg aatttaaccg
    27421 ctacgagatt gagcatgaca gctttggcgg ttttattccg gtgcgttgct gggtgacgga
    27481 tgactttaaa ctggtgctca acctcttcac cagtgatgaa ctttacgatc gccgtaatga
    27541 cccaaatgaa atgcataacc tgatcgatga tgcccatttt gccgacgttc gcagcaaaat
    27601 gcatgatgcc ttattggatt atatggacaa aattcgcgat ccgttccgca gctaccaatg
    27661 gagcctgcgt ccgtggcgta aagatgcacg gccgcgctgg atgggggcgt ttcgtccacg
    27721 cccacaagat ggctattcgc cagtggtacg tgactatgac accggcctgc cgacacaagg
    27781 ggtgaaagtg gaggataaaa aacagaagtt ctgacgataa tgcccgctga tgatcacccg
    27841 gcgggcatta ttcaggctgc ttaaaccagt gaaccgccgg tcaggaagga aaagataagg
    27901 aatatcagca aaatgctgca cacgatgccg ataccaaaaa gcaaggtatg gaaagccagc
    27961 gtcccgccgc caaaaaccag cgcgccgcga atgccccagc gattttcctt acgcataccg
    28021 ccaataaatg cgcaaagtag cgcagcaatg gccagtgcga taccgctttg atcgaggatt
    28081 ttatcgacat ctacgtcctc ttttttctcc acggtggtaa ttttttcgcc ttttattccg
    28141 gcgagaagac ctcttttaat ttcagccgtt ttgtccgcca cgatgctttc aagcgttggc
    28201 ggtggtgcgg agaaagggcc gaatgaaaag tggataatcc ccatcattaa agcgatggca
    28261 ccgaatagca tgcccaatga actgatttta ttttgcgcta tgatacccat ctgacctcct
    28321 tgtagcggat ggcaaaagca tatcaggaaa cgggcggacg aaattcttaa aaaaaaccgc
    28381 taaaggtaga gcgttagtga aattgcaaag gagcaacaat gaaaatttcc cgcctcggag
    28441 aagcgcccga ttaccgcttc tcgctggcta atgagcgtac ttttctggcg tggatccgta
    28501 ctgcactagg atttctggcg gcgggggttg ggcttgatca acttgcgcca gatttcgcca
    28561 cgcccgtcat tcgcgaactg ctggcgttgt tgttgtgtct gttttccggc gggttagcga
    28621 tgtatggcta cttgcgctgg ttacgcaatg aaaaagcgat gcgtctgaaa gaggacctgc
    28681 cctacaccaa cagcttatta atcatcagct taatattgat ggtcgtagca gtgatcgtta
    28741 tgggactggt gttgtatgcc ggatagccgc aaagccagac gcattgccga cccagggctg
    28801 cagccggagc gtacatcatt ggcgtggttt cgtaccatgc tgggctacgg cgcgttgatg
    28861 gcgttggcta tcaaacacaa ctggcaccag gcgggcatgt tattctggat ttcgattggc
    28921 atcctcgcca tcgtggcgct gatcctctgg cactacactc gtaatcgcaa tttaatggat
    28981 gtcacgaata gcgatttttc ccaatttcac gtagtccgtg acaaattttt gatctccctc
    29041 gcggtgttat ctctcgcaat actgtttgct gtaacgcata tacatcaact tatcgtattt
    29101 attgagagag tcgcatgaca ggaagtcagg tcattgatgc tgaagaggac aggcataaac
    29161 tggttgtaga gtataaggat actctgcaac ctgccgattt ttatcacaat ttcaaacagc
    29221 ggggcatccg ctccgtacag cttattcccc atcttgaatt tgatgaccgg ggcgatatga
    29281 cggccgcttc ggtgacagca gagctgtggg ggaaattttt aattgccctg tttgagtgtt
    29341 gggtgcgggc agatatcagt cgtatttcga ttgagctttt tgacgccact ttacaaaaat
    29401 ggtgtggcag cgaaaacccg cacccgcgcc gtgactgcca ggcgtgcgac tggcatcggc
    29461 tgtgtccgca cgctcgggag gagacgccag acaacgtgct ttgtgcgggc tatcaggcgt
    29521 tttattctta ctccgcaccg catatgcggg tgatgcgtga tctgataaag cagcatcgtt
    29581 ctcccatgga gctgatgacg atgctgcggt gatgtgcgct taaacgggct gcccctgatg
    29641 cgacatccgc gtcgccagcg gtagccagca cagcacgatc aacaatccca ttaaggtcat
    29701 caacaacccc aggctgccct gaccggtttg cggcaacatc gctgagagcg acgccagcac
    29761 gccggaacca atgttttgca gaccaccgac cagcgcgcca gccgtgcccg ccaggaaggg
    29821 gaacggctcc atcgcgccgc tggtcgccag cggaaacagc atcccggcac cgaaaaagaa
    29881 tagcgcggcg ggaacaagca gcgtccagac attcatcacg ccaaaccagt cggggatcca
    29941 catcagcaag ccagccagca ggcagcagat aaccgactgc cacatcaacg ttgagaagcg
    30001 tttattagga cgcccggcaa accatgcccc aaaaaatgcc gccggaatcg gcagaataaa
    30061 caaaatactg accgtcatac tgctcagccc taacaccgcg cccatcagca cgccggagca
    30121 ggcttcaaag gcggcaatcc cggccagacc gccaatcagc atcagcaaat aacagttaaa
    30181 accgccgtta ccgaaaagcg ttttataact ggtaagcagg cgcgtgcgcg gtgcatcgac
    30241 cggacgcgtt tccggcatcc agcgggccat actgaaggtc acgccagcac aaagaaccaa
    30301 caaaaagaga taacaggcgc gccagttcca catcgtatcc agcagaccgc cgattagcgg
    30361 tgcgagcaac ggactaacaa gaatccccat gtttaacagg ctgttagcat ggcgcaactg
    30421 tgtccgttca tataaatcac gcggcaaagt acgcgccatt acgccgccaa cgccggtgcc
    30481 catcccctgc atcgcgctgg cggcaatcaa taccgtcaaa ctggaggtcg tgaccgcgac
    30541 cagcgttgcc agcataaaaa tggacattcc gacgaggatc accggtcggc gacccacacg
    30601 gtcggaaatc gggccataaa acagctgtga gacaccgtaa gtcagcagat aagcgcccat
    30661 tacgctctgc accgcccctt cacggacgtt gagatcgcgc gccatatcgg caatagctgg
    30721 aatataaatg gtttgcgcca tctgaccgac ggccacgagt aataccaaca tcaataacaa
    30781 attgacgttt ctatgccttt tcattatcac ggatgctttt ataaaaataa ttagaataag
    30841 tcactacgca ttcccataaa tgcgggagag gaatctatca cattgatgaa tgttagccag
    30901 atatgtgcca ggaatggcga aaagaaaaac ggcagaatat gtaaacaaaa gcggcaataa
    30961 atgttgccgg aagggatggt aagtcggata agatgcgtca gcatcgcatc cgacaccaac
    31021 ccacaagcag aatataaaag gggagcggtt tcccgctccc ctttggtgcg acttgaatct
    31081 gaattacttc aggtatttca gaacagcatc aagtagttgc agtgctgcaa caatgagttt
    31141 gaggataaga atggcgatat ccaccaggct catacgcgtc tcctgtggtt caggagcact
    31201 gccagctgac gtacctttcc gctgcctgtt gccagctgtt gtgcacgcgg tatctcaagg
    31261 agaaacgttg tgcttgttga cataacacag tgtgctcacg cggggttaag gcgctgacgg
    31321 caccacccgt ttcagctagg acttagtgcg ccgggtaacg atgcggcccc cgcataacac
    31381 attgcgtaca gtgatattat agaagtttac tgtataaata aacagtaata gttggacaaa
    31441 acgcaaactg tgatcgatac tcatctatgc actaaatacg tcaaaattcg tgccgaaatt
    31501 gcgcgttctg cgcggaacag gtatactttc agtgttgaca taatacagtg tgctttgcgg
    31561 ttaccagccg caggcagctg acgaaacctc gctccggcgg ggttttttgt tacctgcaat
    31621 tcagtataaa acgtgatcaa cccctcaatt ttcccttgct gaaaaatttt ccattgtctc
    31681 ccctgtaaag ctgtgcttgt ataaatattg ttaaacacaa aaccaacaag gtccccaatg
    31741 actacttcca tgttcaacgc aaagctacta ccaactgcgc catccgccgc agtggtcgtc
    31801 gtgcgtgtgg tggtggtcgt cggcaatgcg ccgtagggac tggaacaaca cacgattcca
    31861 aaaccccgcc ggcgcaaacc gggcggggtt tttcgtttaa gcacctcccg gaaagtcggc
    31921 ccagaaaaaa aggactggag catggcaagt tcgggcacaa catcgacgcg taagcgcttt
    31981 accggcgcag aatttatcgt tcatttcctg gaacagcagg gcattaagat tgtgacgggc
    32041 attccgggcg gttctatcct gcctgtttac gatgccttaa gccaaagcac gcaaatccgc
    32101 catattctgg ctcgccatga acagggcgcg gggtttatcg ctcagggaat ggcgcgcacc
    32161 gacggtaaac cggcggtctg tatggcctgt agcgggccgg gtgcgactaa cctggtgacc
    32221 gccattgccg atgcgcggct ggactccatc ccgctgattt gcatcactgg tcaggttccc
    32281 gcctcgatga tcggcaccga cgccttccag gaagtcgata cctacggcat ctctatcccc
    32341 atcaccaaac acaactatct ggtcagacat atcgaagaac tcccgcaggt catgagcgat
    32401 gccttccgca ttgcgcaatc aggccgccct ggcccggtgt ggatagacat tcctaaggat
    32461 gtacaaacgg cagtttttga gattgaagct cagcccgcgg tggcagaaaa agccgctgca
    32521 cccgccttta gcgaagaaag cattcgtgac gcggctgcga tgattaacgc cgccaaacgc
    32581 ccggtgcttt atctgggcgg cggtgtgatc aatgcgcctg tgcgggtgcg tgaactggcg
    32641 gagaaagcgc aactgccaac taccatgacc ttaatggcgc tgggtatgct gccaaaagcg
    32701 catccgctgt cgctgggtat gctggggatg cacggcgtgc gcagcaccaa ctatattttg
    32761 caggaggcgg atttgttgat tgtgctcggt gcgcgttttg atgaccgggc gattggcaaa
    32821 accgagcagt tctgcccgaa tgccaaaatt attcatgtcg atatcgaccg cgcagagctg
    32881 ggtaaaatca agcaaccgca tgtggcgatt caggcggatg ttgatgacgt gctggcgcag
    32941 ttgatcccgc tggtggaagc gcaaccgcgt gcagagtggc accagttggt ggcggatttg
    33001 cagcgcgaat ttccgtgtcc aatcccgaaa gcgtgcgatc cattaagcca ttacggcctg
    33061 atcaacgccg ttgccgcctg tgtcgatgac aatgcgatta tcaccaccga cgttggtcag
    33121 catcagatgt ggaccgcgca agcctatccg ctcaatcgcc cacgccagtg gctgacctct
    33181 ggcgggctgg gcacgatggg ctttggcctg cctgcggcga ttggcgcggc gttggcgaac
    33241 ccggatcgca aagtgttgtg tttctccggc gacggcagcc tgatgatgaa tattcaggag
    33301 atggcgaccg ccagtgaaaa ccagctggat gtcaaaatca ttctgatgaa caacgaagcg
    33361 ctggggctgg tgcatcagca acagagtctg ttctacaagc agggcgtttt tgccgctacc
    33421 tatccgggaa aaatcaactt tatgcagatt gccgccggat tcggcctcga aacctgtgat
    33481 ttgaataacg aagccgatcc gcaggctgca ttgcaagaaa tcatcaatcg ccctggtccg
    33541 gcgctgatcc atgtgcgtat tgatgccgaa gaaaaagtgt acccgatggt gccgccaggt
    33601 gcggcgaata ctgaaatggt gggggaataa gccatgcaaa acacaactca tgacaacgtg
    33661 attctggaac tcaccgttcg caaccatccg ggcgtaatga cccacgtttg tggccttttt
    33721 gcccgtcgcg cttttaacgt tgagggcatt ctttgcttgc cgattcagga cagcgacaaa
    33781 agccacattt ggctactggt caatgacgac cagcgtctgg agcagatgat aagccagata
    33841 gacaagctgg aagatgtggt gaaagtgcag cgtaatcagt ccgatccgac gatgtttaac
    33901 aagattgcgg tgttttttca gtaaggagcg acattaaacc gctcgggggg gtactgatga
    33961 gcgatttttg atagatccgt tcagatatat agggtgaaat tgtgccagag accatctcag
    34021 gcacaattta ttagccctga atgttaattt aagtcgctgc accagtaatt tgggggttca
    34081 cagttagggc taaaatttcc agcgatggtg ttaaattctg attgctgtct ataccaatgg
    34141 tcatgaccag cattctatta attaatggtt catcgtgagg tatattatgg caagccctat
    34201 aaacagcgga ataatgatgt caaatctctg ctcttccacc tttttggata aaaatataaa
    34261 tgtcgctgct gagcttgata ttacaaatag cgagaaaaaa tattcgcccg gcagtaattt
    34321 tgcaaaatgg atgttgcagg aaataaagcg cttaatagca aatattatgt ccggttctcg
    34381 cagtgttaac accgaaatcc ttaactattt ccaccctatg ccaggcactg aaaataatgg
    34441 caatcgtacc tggatggcgg caactggaga gaacgaatat accgtaatcg aacaaacagg
    34501 tggcaaatcc tttaaagtta tacagtttga tgcaggtgaa aaaaatacaa aaaaaatagt
    34561 aaaagaaaca tcatattctg gagtggctgt ggcgactatt atcaaatcgc tgtctgaaaa
    34621 aactgctgcg ttagaaacac attctgctga cacggttttg aggaaaaaat tagttaatag
    34681 tattgttatg aagaatacgg attttaaaca cgtaattact gcagatattg agaataatat
    34741 atatgattta ttaaagttga gagttcagca ggacgagggg aatttacctg ttcagaaaga
    34801 tcctgaattt cctgatgatt gcttcgattc tatgcgggca gatgctcata gatgtgtaaa
    34861 agatcaagaa caaaaaaaat ctggcacaaa aataaatagc ggcaccaaaa ttacttgttg
    34921 cggtgttgag ttaagtaaag atattcttaa aatattagca gataaaacta aaaaatatga
    34981 gaccgctgtt gaacataccc ttggactcgt ggttgcagac gtatgcttta aagaagatga
    35041 gtgttctcag gtaagagagt tattaaaaaa accaaacggt aacgttacgg atgataagat
    35101 tgatgaaata gtcgcttgct tagccag
//
LOCUS       DAZKFU020000015        35041 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZKFU020000015 DAZKFU020000000
VERSION     DAZKFU020000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.015"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..678
                     /locus_tag="ACHOQQ_RS03045"
                     /old_locus_tag="pgaptmp_000609"
     CDS             82..678
                     /locus_tag="ACHOQQ_RS03045"
                     /old_locus_tag="pgaptmp_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4468909.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIEPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            765..1490
                     /locus_tag="ACHOQQ_RS03050"
                     /old_locus_tag="pgaptmp_000610"
     CDS             765..1490
                     /locus_tag="ACHOQQ_RS03050"
                     /old_locus_tag="pgaptmp_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4468910.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVAPFSQASFGVTGLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1532..4132
                     /locus_tag="ACHOQQ_RS03055"
                     /old_locus_tag="pgaptmp_000611"
                     /pseudo
     CDS             1532..4132
                     /locus_tag="ACHOQQ_RS03055"
                     /old_locus_tag="pgaptmp_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
     gene            4146..4694
                     /locus_tag="ACHOQQ_RS03060"
                     /old_locus_tag="pgaptmp_000612"
     CDS             4146..4694
                     /locus_tag="ACHOQQ_RS03060"
                     /old_locus_tag="pgaptmp_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4468911.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVQD
                     GGPVVTGSNGEYSLTIPDVGLDKVVTGDVAAQANFKLVASDCSNGYSKIFTTLTGTTT
                     SGKLIVNEASSGATGVGMGIKRRDTADSTFFTPNNTDKFEWTADEKTNGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4709..5323
                     /locus_tag="ACHOQQ_RS03065"
                     /old_locus_tag="pgaptmp_000613"
     CDS             4709..5323
                     /locus_tag="ACHOQQ_RS03065"
                     /old_locus_tag="pgaptmp_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4468912.1"
                     /translation="MKLKVIATLIATVAVGVSVSNNLTYASTTSASLTVNSNLTMGTC
                     SAKVVDTSGAVIDTVVLGNVYISELVAKSKVQPFKIRFSDCAGLPGKSAQLVLAPRAN
                     VGCAGGMSNTAAFSNQLNSTTDGGSSRAAVEVWTTTSPEGNGSEQFNCYQRNVINVDL
                     SNASTTQPYDFDLSARMIIAEGWSVNDVLPGRFLSPTTFTITYQ"
     gene            5344..5934
                     /locus_tag="ACHOQQ_RS03070"
                     /old_locus_tag="pgaptmp_000614"
     CDS             5344..5934
                     /locus_tag="ACHOQQ_RS03070"
                     /old_locus_tag="pgaptmp_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4468913.1"
                     /translation="MLCRHHKFVRFLGLATALITPFAYAGQDVDLTAQIVASTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQVNNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            5946..7058
                     /locus_tag="ACHOQQ_RS03075"
                     /old_locus_tag="pgaptmp_000615"
     CDS             5946..7058
                     /locus_tag="ACHOQQ_RS03075"
                     /old_locus_tag="pgaptmp_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4468914.1"
                     /translation="MQRKGNKLLIQLCSAILLFFTTSWYALANECYIERNAEGPYDMV
                     INPKPFSIQSAMVTVPEEIGFATWDSYIDLRGDKIGCKSLGSNTSSVHFLNTADASLL
                     STYTTSHGDALLKTTVPGIVYSVELVCTSCGAAEELDLYLPAQSGADNHTDNASSKWA
                     YEYSDESWYLRFRFFWTPEFKPQNGVSEGTAIPGKIASWYIGNNDQPWINFFVDTSSV
                     HFYVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKI
                     TVKLKAANKPSDATLVGKTNGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAVWSN
                     NLLLLPFTAQLVPDGSGAAVGVGTFSGNATFSFTYE"
     gene            7194..7988
                     /gene="panB"
                     /locus_tag="ACHOQQ_RS03080"
                     /old_locus_tag="pgaptmp_000616"
     CDS             7194..7988
                     /gene="panB"
                     /locus_tag="ACHOQQ_RS03080"
                     /old_locus_tag="pgaptmp_000616"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGU4468915.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALVIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8000..8851
                     /gene="panC"
                     /locus_tag="ACHOQQ_RS03085"
                     /old_locus_tag="pgaptmp_000617"
     CDS             8000..8851
                     /gene="panC"
                     /locus_tag="ACHOQQ_RS03085"
                     /old_locus_tag="pgaptmp_000617"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGU4468916.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLQEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8947..9156)
                     /locus_tag="ACHOQQ_RS03090"
                     /old_locus_tag="pgaptmp_000618"
     CDS             complement(8947..9156)
                     /locus_tag="ACHOQQ_RS03090"
                     /old_locus_tag="pgaptmp_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4468917.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYQEELQKVRQEFAQRFLSK
                     GMSREDVAEVTTLPLTEIDKLINSN"
     gene            complement(9225..10097)
                     /gene="rpnC"
                     /locus_tag="ACHOQQ_RS03095"
                     /old_locus_tag="pgaptmp_000619"
     CDS             complement(9225..10097)
                     /gene="rpnC"
                     /locus_tag="ACHOQQ_RS03095"
                     /old_locus_tag="pgaptmp_000619"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGU4468918.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEERQEFVLRLLNKGMSREDVAE
                     MANLPLAEIDKLIN"
     gene            10371..10751
                     /gene="panD"
                     /locus_tag="ACHOQQ_RS03100"
                     /old_locus_tag="pgaptmp_000620"
     CDS             10371..10751
                     /gene="panD"
                     /locus_tag="ACHOQQ_RS03100"
                     /old_locus_tag="pgaptmp_000620"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGU4468919.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10755..11984)
                     /gene="yadE"
                     /locus_tag="ACHOQQ_RS03105"
                     /old_locus_tag="pgaptmp_000621"
     CDS             complement(10755..11984)
                     /gene="yadE"
                     /locus_tag="ACHOQQ_RS03105"
                     /old_locus_tag="pgaptmp_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU4468920.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12048..12488)
                     /gene="yadI"
                     /locus_tag="ACHOQQ_RS03110"
                     /old_locus_tag="pgaptmp_000622"
     CDS             complement(12048..12488)
                     /gene="yadI"
                     /locus_tag="ACHOQQ_RS03110"
                     /old_locus_tag="pgaptmp_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4468921.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12593..13363)
                     /gene="yadH"
                     /locus_tag="ACHOQQ_RS03115"
                     /old_locus_tag="pgaptmp_000623"
     CDS             complement(12593..13363)
                     /gene="yadH"
                     /locus_tag="ACHOQQ_RS03115"
                     /old_locus_tag="pgaptmp_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4468922.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13360..14286)
                     /gene="yadG"
                     /locus_tag="ACHOQQ_RS03120"
                     /old_locus_tag="pgaptmp_000624"
     CDS             complement(13360..14286)
                     /gene="yadG"
                     /locus_tag="ACHOQQ_RS03120"
                     /old_locus_tag="pgaptmp_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4468923.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14395..15057
                     /gene="can"
                     /locus_tag="ACHOQQ_RS03125"
                     /old_locus_tag="pgaptmp_000625"
     CDS             14395..15057
                     /gene="can"
                     /locus_tag="ACHOQQ_RS03125"
                     /old_locus_tag="pgaptmp_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGU4468924.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15098..15634)
                     /gene="hpt"
                     /locus_tag="ACHOQQ_RS03130"
                     /old_locus_tag="pgaptmp_000626"
     CDS             complement(15098..15634)
                     /gene="hpt"
                     /locus_tag="ACHOQQ_RS03130"
                     /old_locus_tag="pgaptmp_000626"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGU4468925.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15840..18230
                     /gene="gcd"
                     /locus_tag="ACHOQQ_RS03135"
                     /old_locus_tag="pgaptmp_000627"
     CDS             15840..18230
                     /gene="gcd"
                     /locus_tag="ACHOQQ_RS03135"
                     /old_locus_tag="pgaptmp_000627"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGU4468926.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRYSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            18269..18367
                     /locus_tag="ACHOQQ_RS03140"
                     /old_locus_tag="pgaptmp_000628"
                     /pseudo
     CDS             18269..18367
                     /locus_tag="ACHOQQ_RS03140"
                     /old_locus_tag="pgaptmp_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18432..19982)
                     /gene="cueO"
                     /locus_tag="ACHOQQ_RS03145"
                     /old_locus_tag="pgaptmp_000629"
     CDS             complement(18432..19982)
                     /gene="cueO"
                     /locus_tag="ACHOQQ_RS03145"
                     /old_locus_tag="pgaptmp_000629"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGU4468927.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20148..20495
                     /gene="yacC"
                     /locus_tag="ACHOQQ_RS03150"
                     /old_locus_tag="pgaptmp_000630"
     CDS             20148..20495
                     /gene="yacC"
                     /locus_tag="ACHOQQ_RS03150"
                     /old_locus_tag="pgaptmp_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGU4468928.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20601..21467
                     /gene="speE"
                     /locus_tag="ACHOQQ_RS03155"
                     /old_locus_tag="pgaptmp_000631"
     CDS             20601..21467
                     /gene="speE"
                     /locus_tag="ACHOQQ_RS03155"
                     /old_locus_tag="pgaptmp_000631"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGU4468929.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21483..22277
                     /gene="speD"
                     /locus_tag="ACHOQQ_RS03160"
                     /old_locus_tag="pgaptmp_000632"
     CDS             21483..22277
                     /gene="speD"
                     /locus_tag="ACHOQQ_RS03160"
                     /old_locus_tag="pgaptmp_000632"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGU4468930.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22315..22677)
                     /gene="yacL"
                     /locus_tag="ACHOQQ_RS03165"
                     /old_locus_tag="pgaptmp_000633"
     CDS             complement(22315..22677)
                     /gene="yacL"
                     /locus_tag="ACHOQQ_RS03165"
                     /old_locus_tag="pgaptmp_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGU4468931.1"
                     /translation="MDYEFLRDTTGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22852..25449)
                     /gene="acnB"
                     /locus_tag="ACHOQQ_RS03170"
                     /old_locus_tag="pgaptmp_000634"
     CDS             complement(22852..25449)
                     /gene="acnB"
                     /locus_tag="ACHOQQ_RS03170"
                     /old_locus_tag="pgaptmp_000634"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGU4468932.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEKEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25804..27651
                     /gene="yacH"
                     /locus_tag="ACHOQQ_RS03175"
                     /old_locus_tag="pgaptmp_000635"
     CDS             25804..27651
                     /gene="yacH"
                     /locus_tag="ACHOQQ_RS03175"
                     /old_locus_tag="pgaptmp_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGU4468933.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAILAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHDDDYHHHDGGHRDGNGWQHNGD
                     NINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSATQ
                     LPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFSRR
                     QPLTQQQKDAARQRYQSASPEPRQAVRERMQTNPQNQQRREAARERIQPASPEQRQAV
                     REKMQTNPQIQQRREAARERIQSASPEQRQVFKEKVQQSPLNQQQRDNARQRVQSASP
                     EQRQVFREKVKESHPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27722..29146)
                     /gene="lpdA"
                     /locus_tag="ACHOQQ_RS03180"
                     /old_locus_tag="pgaptmp_000636"
     CDS             complement(27722..29146)
                     /gene="lpdA"
                     /locus_tag="ACHOQQ_RS03180"
                     /old_locus_tag="pgaptmp_000636"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGU4468934.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29354..31246)
                     /gene="aceF"
                     /locus_tag="ACHOQQ_RS03185"
                     /old_locus_tag="pgaptmp_000637"
     CDS             complement(29354..31246)
                     /gene="aceF"
                     /locus_tag="ACHOQQ_RS03185"
                     /old_locus_tag="pgaptmp_000637"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGU4468935.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31261..33924)
                     /gene="aceE"
                     /locus_tag="ACHOQQ_RS03190"
                     /old_locus_tag="pgaptmp_000638"
     CDS             complement(31261..33924)
                     /gene="aceE"
                     /locus_tag="ACHOQQ_RS03190"
                     /old_locus_tag="pgaptmp_000638"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGU4468936.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34085..34849)
                     /gene="pdhR"
                     /locus_tag="ACHOQQ_RS03195"
                     /old_locus_tag="pgaptmp_000639"
     CDS             complement(34085..34849)
                     /gene="pdhR"
                     /locus_tag="ACHOQQ_RS03195"
                     /old_locus_tag="pgaptmp_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGU4468937.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYARREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8596 a         9266 c         8593 g         8586 t
ORIGIN      
        1 cccctattac atcctccaaa aactttgagt aatatccgct cgcaatatta atttcatata
       61 atttttgtaa ggatgcatgt aatgaaaaaa gcacttctcg cagccgctct ggttatggct
      121 tctggttccg ccctggctgt agatggtggt catatcgact ttaacggtat ggtacagtcc
      181 ggtacctgta aagtgggtgt ggtagatact ggtatgcaca gcgttaccac tgatggcgtg
      241 gttaccctgg atactgcgaa tgttactgat acttttgctg aagttagcgc aactgctgtc
      301 ggtttactgc cgaaagagtt catgatttct gttgagtgtg atccaggtgc tccgaagaat
      361 gctgagttaa ctatgggttc tgcaagctac gcgaacacca gcggtaccct gaataacaat
      421 atgaacatca ctgttaacgg tattgaaccg gctcagaacg taaacattgc agttcataac
      481 atgaaaaaca aagctggcgc tgctgaaatt aagcaggtcc atatgaacaa ctcttctgaa
      541 gttcaggaac tgacattaga cgcagaaggt aaaggccagt acgtatttaa cgcatcttac
      601 gttaaagcac cgaacagccc ggctgtaact gctggtcatg taaccactaa cgcgctgtac
      661 accgttgctt ataagtaatt gatggtgtgt tgagcataag gtttgccagc aggcaaacct
      721 tattttcgcg aatttaatct ttctgcagac agcaaggagt gcttatgttt cccttggtta
      781 aaaaaacggt tagtgcatta tttgtgagta ctctgttagc cagtgcacca gcattcgctg
      841 acattattat ttccggaaca cgtatcattt ataacgctga taaaaaagat gttaacgttc
      901 gtctggaaaa taaagggaat cgccccttac tgattcagaa ctggttggat actggcgacg
      961 acaatgccga tcccagccag atcaaagtgc cgtttaccgc gaccccacca gtttcacgtg
     1021 tggagccaaa acgcggtcaa accgtaaaag tgatgtatac cggtgcaaca gccctgccga
     1081 aagaccgtga aagcgtctac tggtttaacg tgctggaagt gccgcctaag ccgaaagatg
     1141 ctgaggctga caaaaacttg ttacagctgg ctttccgcac acgcatcaaa ctcttttatc
     1201 gcccgagtgg gttacaggga gaaccggctg aagcaccggc gaaaatcacc tggaaattaa
     1261 acaacgctca gttacaggca aataacccaa cgccatatta cgtgtccttt aatgaggtca
     1321 agctggaaag cggcggcaaa acctacaacg tcaattccag catggtcgcg ccgttcagcc
     1381 aggcaagctt tggcgtaacc ggtctgccag gaagcgtgtc atccggaaaa gtggttttta
     1441 aagcgatcaa cgactttggc ggcaacattg atggttccgc aacattctaa aaaataaacg
     1501 gattcttttt aacatcgcca ggaatgcaac cttgtaccag tttactcatc aaaaaagccg
     1561 tatcccgaaa aaaacgctac ttgcggcctg ttgcgccctt ttttacagcg gcaacagttc
     1621 tgcggcagac acggtggaat atgacagttc cttcttaatg ggaactggcg catcaacgat
     1681 tgatgttaaa cgttatgctc aaggcaaccc gacaccgccg ggtctctata atgtccgcgt
     1741 gtttgtaaac ggtcaggcga cgtccagctt agatattccg tttgtggata ttggcgaaaa
     1801 cagcgcagcg gcctgtatta cccacaaaaa cctggcacaa cttcacatta agcaacctga
     1861 acagcctgtc actttactcg ccagagaagg tgaggaagag gattgtctgg atttggcaaa
     1921 gtcatacgaa aaggcggatg tgcgctttga cggtagtgac cagtttctcg atctgacgat
     1981 ccctcaggcc tatgttttga aaagctatgg cggctacgtt gacccttctt tatgggaatc
     2041 gggaattaac gctgccacac tggcatatac cctgaacgcg tatcacacaa gttcagataa
     2101 cgacaatagt gacagcgtct atggcgcatt taactcgggt atcaatttag gagcctggca
     2161 cttccgtgcg cgcggtaact ataactggac aacagataac ggcagcgatt tcgatttcca
     2221 ggatcgttac ttacagcgtg acattccggc aatccgttca cagataatta tgggtgacgc
     2281 ctacaccacc ggtgaaacgt ttgactctgt caacgtccgt ggtgttcgcc tgtacagcga
     2341 tagccgtatg ctaccttcgg cgctggccag ttacgccccg accatccgcg gtgtagcaaa
     2401 ctccaacgcc aaagttacgg tgacgcagaa cggttataaa atctatgaaa ctaccgttcc
     2461 gcccggtgaa tttgttatag acgacattag cccctccggc tttggtagcg aactggtcgt
     2521 aaccattgaa gaagcggatg gttccaaacg cacctttacg caacccttct cgtcggttgt
     2581 gcaaatgcaa cgtcctggtg taggtcgttg ggatttcagt gccggtaaag tcattgatga
     2641 tagcctgcgt tccgaaccca atatggggca agcctcttat tactatggtc tgaataacct
     2701 cttcacgggt tataccggca ttcagttcac cgataacaac tatcttgccg ggctgttagg
     2761 tgtgggtatc aacaccagca tcggcgcctt tgcggtagac gttacccatt cccgtgctga
     2821 aattccggat gataaaacct accaggggta aagttatcgc gtgacctgga acaaactttt
     2881 ccaggatacc gggacatcat ttaacctcgc ggcgtaccgc tattccaccc aggattacct
     2941 gggcctgcat gatgcgttag tcctcattga cgacgccaaa catttgtctg ccgatgaaga
     3001 caaaaacacc atgcagacgt actcacgtat gaaaaaccag tttaccgtca gcatcaacca
     3061 gccgctgaaa acgacttacg aagattacgg ttcactgttt atttccggta gctggacgga
     3121 ttactgggcg gcgaacaata gccgcactga atataatgtt ggttacagta aaagcgtttc
     3181 gtggggcagt ttcagcgtca acctgcaacg tagctggaat gaagacggcg agaaagatga
     3241 cgcgatgtac gtcagcgtta gcgtacctat tgagaatatt ttaggtggca agcgcaaatc
     3301 ttctggtttc cgcaatttaa atactcagct caataccgat ttcgatggtt cacatcagtt
     3361 gaatgttaac agttccggta acactgaaaa caatctggtg aactacagtg tcaacgctgg
     3421 ttatagcctc gataaaaact ccggcgattt agcctctgtt ggcggttatc tcaactatga
     3481 gtctgggtta ggcggtattt ccgcttcggc ctcggccact tctgataata gccagcagta
     3541 ctccatctcc accgatggcg gctttgtatt acacagtggt ggtttaacgt tcactaacaa
     3601 cagtttcagc agtaacgata cgctggtgtt aatcaacgcc ccaggtgcta aaggcgcacg
     3661 aatcaataac agtaataacg aaatcgatcg ctggggatat gccgtgacgt cctctgtcag
     3721 cccctaccgt gaaaaccggg taggtctgaa cattgaaaca ctggaaaacg atgttgaact
     3781 gaaaagtacc agcgcaacca ccgtaccacg cagcggctcc gttgttttga cccgtttcga
     3841 aactgacgag gggcgttctg ccgtgctgaa tattactgcc gccaatggca aatccattcc
     3901 gtttgctgcg gaggtttacc agggtgaggt gatgatcggc agcatgggcc agggtggtca
     3961 ggcatttgtg cgcggtatta acgacagcgg ggaattaaca gtgcgctggt atgaaaacaa
     4021 ccaaaccgtt gactgtaagt tgcactacca gttcccggcg cagccacaaa cgctgggaag
     4081 taccaacacc ttattactta acaatcttac ctgtcaggta gcaaatcact aatatggaaa
     4141 attctatgaa aaggatacta ctaacatccg cgttaatagg tctgggttta ccggcggttg
     4201 gctcagcaac agatcttaat gtcgatttta cggccaccgt attagccacc acctgtacga
     4261 taactatcgt acaagatggt ggtccagtag tgacgggtag caacggtgaa tatagcctga
     4321 caattcccga tgttggcctg gataaggttg tcactggaga tgttgcagct caagccaact
     4381 ttaaattagt ggcttccgat tgcagcaatg gttattcaaa gatttttacc acacttacag
     4441 gaacaacgac ttccggtaag ttgattgtca acgaagcaag ctctggcgct acgggtgtgg
     4501 gtatggggat taagcgtaga gataccgcag actcgacttt ctttacaccc aataatactg
     4561 acaaatttga atggaccgcc gatgagaaaa ctaacggtgt gcctctgacc gtagcactgc
     4621 gcgaaacaac ggcgggagca ggacgcaccg gtgcgtttca ggcaaaagcc acctttaact
     4681 tcacctacga ataacaggga cagccaccat gaaattaaaa gtcatcgcta cactgattgc
     4741 tactgttgcc gtgggtgtca gcgttagcaa caatctcact tatgcgagta cgacatccgc
     4801 ctctttaacc gtaaacagta acctgactat gggtacctgt agtgcgaagg tagtggatac
     4861 cagtggtgcc gtgatcgaca ccgttgtctt gggcaatgtt tatatttctg aactggtagc
     4921 caaaagcaag gtacaaccat ttaaaatccg cttcagcgat tgtgccggcc ttccaggaaa
     4981 gagtgctcaa ctcgtcttag cgcccagagc aaatgtcggt tgtgcaggcg gcatgtctaa
     5041 cactgcagcc ttttcaaatc aactcaactc caccaccgat ggtggttcaa gcagagcagc
     5101 agtcgaagta tggacgacta caagccccga gggaaatggc agtgagcaat tcaattgtta
     5161 tcagcgaaac gtcatcaatg tcgatctttc taatgccagc acaacacagc cttatgattt
     5221 tgatttaagc gcacgaatga ttatcgctga aggatggtcg gtcaatgacg tattaccggg
     5281 tagattcctc tctcctacga ctttcacaat cacttatcag taatagcgga taaggatatt
     5341 agaatgctat gcaggcatca taaatttgta cgttttttgg ggctggcaac agccctcatt
     5401 acgccttttg cctatgctgg ccaggatgtg gatctcacgg cgcagattgt ggccagcacc
     5461 tgtcaggttg aagtcagtaa taatggcgtt gtcgatctcg gcacagtgac gctggactat
     5521 tttgccgaca atgtaacccc gacaacggat tatgcgggtg gcaaaacgtt taatgtgaat
     5581 gtggtctctt gcgataacat tcaaacgaca cagtcgcaaa tgaaattgga ttttcagccg
     5641 caggcgggat cgcttgcaca ggtgaataat caaattttta gtaatgaata cgaacagcag
     5701 gctaccgggg cgaaaaacgt cggcattgtg atattttcgg cccaaccgaa tcagcagaca
     5761 tttaacgtgc gaggaacgga tggttcctcg acagcaattt attcggtcgc gcctggcaat
     5821 gctgttccct caacctggac cttttattcc cgtatgcagc gggtgaataa tgcccttcct
     5881 cctgaatcgg gaatggtgag aagccaggtt attgttaacg tatcttacga ataataagga
     5941 taattatgca aaggaaaggc aataaactgt tgattcagtt atgcagtgcg atactgctat
     6001 tttttaccac atcctggtat gcattggcga atgaatgtta tatagagaga aatgctgaag
     6061 ggccttatga tatggtgata aacccaaagc ccttcagtat acagtcagcg atggtcacag
     6121 tcccggaaga aataggcttc gcgacctggg attcttatat tgatcttcgg ggcgataaga
     6181 ttggttgtaa atccttaggt agtaacacaa gctcagtgca ttttcttaat actgctgatg
     6241 ccagtctctt atcaacctat actaccagcc atggcgatgc tttattgaaa acaaccgttc
     6301 ccggtattgt ttattcagta gaattagtat gtacaagttg tggagccgcg gaggagcttg
     6361 atttgtatct tcctgcacaa agtggtgcag ataatcatac agataacgcc agctcaaagt
     6421 gggcctatga gtatagtgac gagagctggt atttacgatt tcgttttttc tggaccccgg
     6481 aatttaaacc tcaaaatggg gtttcagaag gaactgcgat accaggtaaa atagcttcct
     6541 ggtatatagg caataatgac caaccatgga taaatttttt cgttgataca agctccgttc
     6601 atttttacgt tgacgaaccg acctgtgcaa cagttgccct cgcacaagat cagggcaacg
     6661 tcagtggcaa ccaggtaacg cttgggaaca gctatgtttc ggaagtgaaa aatgggctta
     6721 cgcgggaaat ccctttttct atccgtgctg aatactgcta tgccagtaaa attacggtta
     6781 agttgaaagc ggcaaataaa cccagcgacg ccacactagt aggtaaaacg aatggctcgg
     6841 cttcaggcgt ggctgtaaaa gtaaattcaa cttatgacaa tagcaaagta ttgttaaaag
     6901 cagatggtag caacacggtt gactataact tcgccgtctg gtcaaacaac ctgctgcttt
     6961 taccttttac ggcgcagctg gtaccggatg gcagtggtgc tgctgtcggt gttggaacct
     7021 tctctggtaa cgcgaccttc tcctttacct acgaataagt aaaacgtcat tccttcctca
     7081 tcgcaccaga gttctgctct ggtgcgaacg ttttccccac actaaaaacc attgcccccc
     7141 tgatttcgac tgttagaatg cgatcaatct gactttagcc atcagggtac gttatgaaac
     7201 cgaccaccat tgcttcattg caaaaatgca aacaggaaaa aaaacgtttc gcgaccatca
     7261 ccgcttacga ctatagcttc gccaaactct ttgctgacga agggctgaac gtgatgctgg
     7321 tgggtgattc gctgggcatg accgttcagg gccacgactc cactctgcca gtaaccgttg
     7381 ccgatatcgc ctaccacact gccgccgtac gtcgcggcgc gccaaactgc ctgctgttgg
     7441 ctgacctgcc gtttatggcg tatgccacgc cggaacaagc ctttgaaaac gccgcaacgg
     7501 ttatgcgtgc cggtgctaac atggtcaaaa ttgaaggcgg tgagtggctg gtcgaaaccg
     7561 taaaaatgct gaccgaacgt gccgttcctg tatgtggtca cttaggttta accccacagt
     7621 cagtgaatat tttcggtggc tacaaagttc aggggcgcgg cgatgaagcg agcgatcgac
     7681 tgcttagcga tgcattagcc ttagaagccg ctggtgcaca gttgctggtg ctggaatgcg
     7741 tgccggttga actggcaaaa cgtattaccg aagcactggt gatcccggtt attggcattg
     7801 gcgcaggcaa cgtcactgac gggcagatcc tcgtgatgca cgatgcgttc ggcattaccg
     7861 gcggccatat ccctaaattt gccaaaaatt tcctcgctga aacgggcgac atccgcgcgg
     7921 ctgtgcggca gtatatggct gaagtggagt ccggcgttta tccgggcgaa gaacacagtt
     7981 tccattaagg agtgacgttg tgttaattat cgaaaccctg ccactgctgc gccagcaaat
     8041 tcgccgcctg cgtatggaag gcaagcgcgt ggcgctggta cctaccatgg gcaacctgca
     8101 tgatggccac atgaagctgg tcgacgaagc caaagcccgc gccgatgtgg tcgtcgtcag
     8161 tattttcgtt aacccgatgc agttcgaccg cccggaggac ctggctcgtt acccacgcac
     8221 cttgcaggaa gactgcgaaa agctgaacaa gcgcaaagtg gatctggttt tcgcgccctc
     8281 ggtaaaagag atctacccga acggtactga aacccatact tacgttgacg ttccagggct
     8341 ttcgaccatg ctggaaggcg ccagccgtcc ggggcatttt cgcggcgtgt cgactatcgt
     8401 cagcaagctg tttaacctgg tccagccgga catcgcctgc ttcggtgaaa aggactttca
     8461 gcaactggcg ctgatccgca aaatggttgc tgatatgggc tttgatattg agattgtcgg
     8521 tgtgccaatt atgcgcgcca aagacggtct ggcactgagt tcccgtaacg gttatctgac
     8581 ggcagaacaa cgcaaaattg cgcccggtct gtacaaagtt ttaagttcga ttgccgacaa
     8641 attgcaggct ggcgaacggg atctcgatga aattattgct attgccgggc aagaactgaa
     8701 tgaaaaaggc ttccgcgccg atgatattca gattcgcgat gccgacacgt tgcaggaggt
     8761 ctctgaaaac agcaaacggg ccgtaattct ggtagccgcc tggcttggcg atgctcgcct
     8821 gatcgacaac aagatggtcg aactggcgta atacttaact ggcgctacgg ctgatggcgc
     8881 cagttattaa tttgccctac gtcatggcga tgacgtctgt aagtgattat tcatatcgct
     8941 ggcaatttag ttagagttaa ttagcttatc aatctctgta agaggtaaag ttgtcacctc
     9001 tgcaacgtct tcccgagaca tccctttact tagaaaacgc tgggcgaatt cctgtcttac
     9061 cttctggagt tcctcttgat atcccttctg aaatcccttc tcttcaaacc actgcgccaa
     9121 cgtctgcctg gtttctctcc gtgtctttat ttccataggt tcccttaatt atctggtcag
     9181 ttaacaataa caattatctg gctataactg atgatgcaga caacttagtt aatcagctta
     9241 tcaatctcgg caaggggtaa atttgccatc tctgcaacgt cttcccgaga cattccttta
     9301 ttcagaagac gcaggacgaa ttcttgcctt tcctcctgtc ttcccctctc ttcaaaccac
     9361 tgcgccagcg tcatcataga ctcccctccc gtttccctgt ctctcagcac accataaaac
     9421 aaatccgctt gttcagtatg accgcgttgc agcatatagt tttgcatggc aactaactga
     9481 cttccgctag tgtacccttc gtcaatcagc gtgaccagtt gctccagcaa taacattaag
     9541 tcgcgctggc gaatatgttt ttgcagtagt tcgagaatcg caatccgccg atgttgcatg
     9601 atttcgtcat ccggtgtgat ggtgatatcc accagcggga aaggactgtt atagacgcgt
     9661 cgcgccagct caggcgagta aaacatatca aaccagcaca ttgagagcgg ataaggtgtg
     9721 gcctcgccct gataaaacag tatcggcacc accagcggca gcttatcgtg gtcagcctcc
     9781 agatgccggt gcatggcggc aatagaataa cgcatcatgc gaaaagccat tttcttatct
     9841 ggcttgcttt gatgttcaat cactacatgc agataaccgg gattgccctg catttgcacg
     9901 gaatagagca cgtccgtgct gtgtcctttc aggctctctt caatgaaact ccccgactct
     9961 aaatgaagcg tgttgaggtc acaaagttct cgtaattcca ctggcaaatg tatctccaga
    10021 aagtcgcgag ccgtctccgc atgcattaaa aattgtttaa ataccgcgtc atgcggtgtg
    10081 gtactcggtg catccatctt ccctgtcttc cgtgttgtct tgcgtgaagg cagtatccag
    10141 tcaaggcgtg ctatcgtcag ccacctcttt agtgtttacc gagcagcgtt cagagaaatt
    10201 tcaattggtt acagccgtta catggcacgc ttcggcgcgg tacgcaaact gctgatgcaa
    10261 atgccgtcag atgagtagac actaaacaaa aatcgggcaa tactgcgtga gaatttcctc
    10321 aaagcaggca ccgcctgctt cgttaacgac agggtagaaa ggtagaagtt atgattcgca
    10381 cgatgctgca gggcaaactc caccgcgtga aagtgactca tgcggacctg cactatgaag
    10441 gttcttgcgc cattgaccag gattttcttg acgcagccgg tattctcgaa aacgaagcca
    10501 ttgatatctg gaatgtcacc aacggcaagc gtttctccac ttatgccatc gcggcagaac
    10561 gcggttcgag aattatttct gttaacggtg cggcggccca ctgcgccagt gtcggcgata
    10621 ttgtcatcat cgccagcttc gttaccatgc cagatgaaga agctcgcacc tggcgaccca
    10681 acgtcgccta ttttgaaggc gacaatgaaa tgaaacgtac cgcgaaagcg attccggtac
    10741 aggttgcttg attgttatcc ctgcggctgg ttactcacca gccgcgacat cgtctccagc
    10801 gaatccgttc ttaagatata aagtcgtttt agtaacaacg gattatcccc cggttttact
    10861 ttgcctttca tggttgtcac cgccaggtga aatccggcat cgtttgctgc cttcacggct
    10921 ttgtcattaa agccgccaaa cggatacgaa agataccaga catgcggatt aaattgcgcc
    10981 agagcgcggc gtgaacgtgc aaaatcaaac agaatattgt gctcactacg gctcagtaat
    11041 atgggtcggc gataaccatc tacccgatgc aaaaaatggg tatgtgactg gaaatcaaat
    11101 acatcgcgaa tttcgttaag ctcagaaacg ctcataaatt gcagcgattt tgggttccac
    11161 ttctgcgggt gacgtttgat gcgtgaggta acaataaacg ccgtcgcctt catgccatat
    11221 tgtttcaaca caggatacgc atagcggctc accgacttga ggccatcatc aaaggtaatc
    11281 accaccgctc gcgcagggag attgatctta ttcttcacgt aaccttccag ctgcaccatg
    11341 ctcagtgtcg cgtatcccct gtcgcgcagc caggccatct ggttattgaa agcgcgtacc
    11401 gaggtggtcg tcgaagtatg gcgaaaacgg gtgttttctt cgtcgcgcag aatatggtga
    11461 taagttagca ccggaaggcc attatcgggt tgggcatcca gcgcgctgat ataggccagt
    11521 cgatcgccaa tacggacctg ataccaggtt tgatttaacc tgtctttcag tttatgcaaa
    11581 atcgggtagc gcaaattgtc cgccagtacc ccaaatggcg cactgcccgc actcggcgcg
    11641 ttatagaccg gcgtatcttt ccaggtaact aagttctgat tactcagcgg cttgttgagg
    11701 tcacccaaac cgtcttcaac tttttgtcgc ccctgaaccg gctcgagatg ccctttatcg
    11761 ataaaccctt taccaaagcc aaaattaaat gcgtaataac ttgcggcagt gggctccacg
    11821 gcaatgattt gtccggcccg aatattcccc acggtcacca tcttgtcacc aatttgcgcc
    11881 cagaccgcag cattttcgat ggtttgcata taacgggcag gtaacgcggc actgacactt
    11941 gcggtaaaca gcatcagcag gagaataaca gcttgtttgt acatgaaaaa ataaccgagg
    12001 gtgaaacaac gcttttattg tagcaaaagc gctataagaa tcgtgggtta atactcatac
    12061 aaattatgtt tgaggacgaa aggcggattt ttttgttgtt ggtgccaaag actactcacc
    12121 tccggcgcac ccagttcgac aatacgctcg cgaaactctg aactggtcat ggtttcacgg
    12181 caagccatca tctgttcaat taacggtaac gtgacaccag aaatcacttc gcaacgggag
    12241 tgtttgtggc ttaataatga agccacgcga tacggtggcg ctccggctat atcggttaag
    12301 aagatgacac cctcaccaga gtccgcttca tgcagagcat cgcacatcat gcggctgagc
    12361 atattagagc ttaacccacg ccagaaattc acggcccggc actgaagaag cgccccatgt
    12421 tttttctcca gcgcatccag tatctcttgc gcccggtcat cgtgacaggt aattacccaa
    12481 cctaacatcg ccttcctcct tagccgaaaa tcagtctacc ctgaccattg ttgataaagg
    12541 gtgatagccg tcaaaactcc tctcccccaa atccagagga gaggaaatag ccttagctac
    12601 gcaaaccacg tccacgttgg atcagcgacc aacagatcaa ataaaacgcc acaataaaga
    12661 ccaccagtac gccaaaggta gtgaccagcg gaacatcatt gataccgagg aagccgtagc
    12721 ggaaaccact gatcatataa acgattgggt tcaggtgcga cagcccttgc cagaacggcg
    12781 gcaacaaagt cagggagtaa aagaccccac ccaaatacgt gagtggcgtt aacacaaagg
    12841 ttggcaccag gctgatgtca tcgaacgttt tggcaaacac accgttcagc aaacccgcaa
    12901 gggagaacaa caccgccgtg agcaccaggg ttaaggcaac gaatacccac gaatgcacct
    12961 gaaacggcac aaaaaacagt gaaattgccg tcaccagaat gccaacaaac agaccacgcg
    13021 ccacgccacc gccgacatat ccggcaataa tgacgtgagt cggaaccggc gctaccagca
    13081 gctcttcaat attacgctgg aatttggcac caaaaaatga tgacgcgacg ttggcgtagg
    13141 cgttggtgat caccgacatc atgatcagcc ccggtacgat gaactgcatg tagctgaagc
    13201 catgcatatc gccaatacgc gaaccaatca ggttaccgaa aataataaag taaagggtca
    13261 tggtgataac tggcggcacc agcgtctgca cccagatacg cataaagcga tggatctctt
    13321 tcgcccagat gctttttaac gccacccagt aaagatgcat catgcgcgat ctccttgttt
    13381 ttcattaacc agtgaaacaa acagctcttc cagacggtta gctttgttac gcatacttaa
    13441 tacctgaatg ccctgctcac ttaactgcgt aaatacgctg ttgatcccct gctcacgcag
    13501 cacttcaact tccagcgtcg cggtatcgac cagtcgatac tgatagccat cgagcttcgg
    13561 taacggactt ttcggtgcga gatcgagaat aaaggtttcc gatttcagct tcgccagcag
    13621 cgccttcatc gaggtatttt ccaccagctc accgtgttga ataatgccga tattgcggca
    13681 cagcatttct gcttcttcca ggtagtgtgt ggtgagaatg atggtggtgc ctttgtcgtt
    13741 taaatccttc aaaaagcccc acattgagcg gcgaagttca atatccacgc ctgcagtcgg
    13801 ctcgtcgaga atcagtagtt taggttcatg cattaacgca cgggcaatca ttaaacggcg
    13861 cttcatcccg ccagataaca tgcgcgcacg ttcgttgcgt tttccccata aatcgagctg
    13921 cttaagatat ttttcgctgc ggatgtacgc ctctttgcgc tccacaccgt agtaccctgc
    13981 ctgattcacc acaatttgct gcacggtttc aaacgggttg aagttaaatt cctgcggcac
    14041 cagtcccaac tgacgtttag cgttcacgac atctttctcg agatcgtaac caaatacgct
    14101 gacccgcccg gaggttttat ttaccagaga gctgataata ccgatagtgg tcgatttccc
    14161 ggccccgttc ggcccgagaa gcgcataaaa atcacccgct tcgacctgca aatctattcc
    14221 acgaagcgcc tgaacgccgc ctggataggt ttttttaagc tgttgaagtt ccagtgcaat
    14281 ggtcataaat ttttacttac cttacgttct tacactttat ctgtggttta aatcgtcccg
    14341 gagttgccct atattagcca aacgtaatta tttggttaca ggtcgttaac ctccatgaaa
    14401 gacatagata cactcatcag caacaatgca ctatggtcaa aaatgctggt ggaagaggat
    14461 cccgggtttt ttgagaaact ggcacaagcg caaaaaccgc gctttctatg gattggatgt
    14521 tccgacagtc gcgttcctgc agaacgttta accggtcttg agccgggcga actctttgtt
    14581 caccgtaatg ttgctaacct ggtcattcac accgacctga actgcctttc cgtggttcag
    14641 tatgcagtgg atgtactcga agttgaacac attattatct gtggccacta cggttgcggc
    14701 ggcgtacaag ccgcagttga aaacccggaa ctggggctta tcaacaactg gctgctgcat
    14761 atccgcgata tctggttcaa acatagctca ttgctcggcg aaatgccgca agagcgccgt
    14821 ctggatacct tgtgtgaact gaacgtcatg gaacaggtgt ataacctggg ccactccacc
    14881 attatgcaat cagcgtggaa acgcgggcag aaagttacca ttcacggctg ggcctatggc
    14941 attcacgacg gcttgctgcg tgatctggat gttactgcca ccaaccgcga aacccttgag
    15001 caacgttacc gtcacgggat ttccaacctc aagctgaaac acgccaacca caaataaaaa
    15061 tgccatgccg gatgcaacac atccggcaac ttcacactta ctcgtccagc agaatcactt
    15121 tgccgatata cggcagatga cgataacgct gtgcgtaatc aataccgtaa cccaccacaa
    15181 actcatccgg gatcgagaaa ccgataaatt ctaccgggac gttcacttca cggcgggacg
    15241 gtttatccag cagcgtacaa atcgccagcg acttcggttc gcgcaggctt aagatctcac
    15301 gcactttcga cagtgtattc cccgagtcga tgatatcttc aacaatcagc acgtccttgc
    15361 cacggatatc ttcatccaga tctttgagga ttttcacatc acgggtggtg gacatgccgc
    15421 taccgtagct ggaggcggtc ataaagtcga cttcatgaga tacctgaact tcacggcaca
    15481 ggtccgccat aaacataaat gagccacgca gcagacccac cagcaccata tcgctgccgc
    15541 tgtctttgta acgctcagta atctgacgac ccagttcggc gatacgcgct ttaatctccg
    15601 cttcggggat cattacttct acagtatgtt tcatatctct aaccatatga tttaaaaaca
    15661 aatcacttcg cacgggcagg ttttggcacc gcaacaaagc gcaagacacg cagtatacca
    15721 gtaaaactat tcattcgcgg aatctgttaa taaagctcga attgtgatga cggtcacaca
    15781 tgttaaaacc tataattaat tgctactaaa atattaatga attgaaatgg tgtctcttta
    15841 tggcaattaa caatacaggc tcgcgacgat tactcgtcac gctaacagcc ctttttgcag
    15901 cgctttgcgg gctgtatcta ctcattggcg gaggctggct ggtcgcgatt ggcggctcct
    15961 ggtactaccc tatcgctggc cttgtgatgc tcggcgtcgc ctggatgctg tggcgcagta
    16021 aacgtgccgc gctttggcta tacgccgccc tgctgctcgg caccatgatt tggggcgtct
    16081 gggaagttgg tttcgacttc tgggcgctga ctccgcgcag cgatattctg gtcttcttcg
    16141 gcatctggct gatcctgccg tttgtctggc gtcgcctggt cattcctgcc agcggcgcag
    16201 ttgctgcgct ggtggtcgca ctgctgatta gcggtggtat cctgacctgg gccggattta
    16261 acgatccgca ggaaatcaac ggcaccttaa gcgccgatgc cacacctgcc gaagctatct
    16321 cacccgttgc cgatcaggac tggcctgcgt atggtcgtaa tcaggaaggt caacgctatt
    16381 cgcccctgaa acagatcaac gccgataacg tccataacct gaaagaagcc tgggtgttcc
    16441 gtactggcga tgtgaagcag ccgaacgatc cgggtgaaat caccaatgaa gtgacgccga
    16501 ttaaagtggg cgacaccctt tacctgtgta ccgctcacca gcgactgttt gcgcttgatg
    16561 ccgccagcgg caaagagaaa tggcattacg atcctgagct gaaaaccaac gagtctttcc
    16621 agcacgtaac ctgccgtggc gtctcttatc atgaagccaa agcagaaacg gcttcgccgg
    16681 aagtgatggc ggattgcccg cgtcgtatca ttcttccggt caatgatggt cgactgattg
    16741 cgattaacgc tgaaaacggc aaactgtgcg aaaccttcgc caataaaggc gtgctcaatc
    16801 tgcaaagcaa tatgccagac accaaaccgg gtctgtatga accgacttcg ccaccgatta
    16861 tcaccgataa aaccattgtg atggcaggtt cagttaccga taacttctca acccgcgaaa
    16921 cgtctggcgt gatccgtggt tttgatgtca acaccgggga gctgctgtgg gcttttgatc
    16981 cgggcgcgaa agatccgaac gcaatcccgt ctgacgaaca cacctttacc tttaactcgc
    17041 caaactcgtg ggcaccagcg gcctatgacg cgaagctgga tctggtgtat ctgccgatgg
    17101 gcgtgaccac gccggatatc tggggcggta accgcacacc ggaacaggaa cgttatgcca
    17161 gctcgattct ggcgctgaat gccacaaccg ggaaattggc gtggagctac cagaccgttc
    17221 accacgacct gtgggatatg gatcttccgg cacagccgac gctggcggac atcaccgtta
    17281 atggtcagaa agtgccagtt atttacgctc cggcgaaaac cggcaacatt tttgtgctcg
    17341 atcgtcgtaa tggcgaactg gtggttccgg caccggaaaa accggttccc caaggtgctg
    17401 ccaaaggcga ttacgttacc cctactcaac cattctcgga actgagcttc cgtccgacga
    17461 aagatttgag cggtgcggat atgtggggag ccaccatgtt tgaccaactg gtgtgccgcg
    17521 tgatgttcca ccagatgcgc tatgaaggca ttttcacgcc gccatctgaa cagggcacgc
    17581 tggtcttccc gggtaacctg ggaatgtttg aatggggcgg gatttccgtt gatccaaatc
    17641 gtgaagtggc aattgccaac ccaatggcac tgccgtttgt ttcgaaactg atcccacgcg
    17701 gtccgggtaa cccgatggag cagccgaaag atgccaaagg cacgggtacg gaatccggca
    17761 ttcagccaca gtacggtgta ccgtatggtg tcacgctcaa cccgttcctc tcaccatttg
    17821 gtctgccatg taaacagcca gcatggggtt atatctcggc gctggatctg aaaaccaatg
    17881 aagtggtgtg gaagaaacgt attggtacgc cgcaggacag tatgccgttc ccgatgcctg
    17941 taccggtgcc gttcaatatg ggtatgccga tgctgggcgg gccaatctcc acggcgggta
    18001 acgtgctgtt tatcgccgct acggcagata actacctgcg cgcttacaac atgagcaacg
    18061 gtgaaaaact gtggcagggt cgtttaccag cgggtggtca ggcgacgcca atgacctatg
    18121 aagtgaatgg taagcagtat gtggtgatct ccgcaggcgg tcacggttca tttggtacga
    18181 agatgggcga ctatattgtg gcttatgccc tgccggatga tgtgaagtaa gacttgctca
    18241 gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc gtgaacgcct tatctagcct
    18301 acaaaattgt gcaaaatcaa tggattgcac aggtagcgta ggcctgataa gcgtagcgca
    18361 tcaggcagat ttgcgtttgt catcagttta atgcccggag agatccgggc atattcccga
    18421 atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg ctccagcaga
    18481 tggcagtgcg ccatataagc atgttctttc ggtgcgtcgt gattaaactt caccagcact
    18541 tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg agccgctggc
    18601 ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa cggatgcagc
    18661 atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc cgccgcaaac
    18721 atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg gaaatcgaac
    18781 ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat catctggctg
    18841 tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg catccccatc
    18901 atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc ttccagcgaa
    18961 ggtaacgcag gcaggctact taatgtgtct ggcaaagcac cggaggcact aatagcaatc
    19021 ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat ccccatctgg
    19081 ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac cagcacttca
    19141 aaacgctcgc ccatcagcac cggcagctcg ttcaccttca ccggttcagg tagcagacca
    19201 ccgtcgctgg caatcacata aagcgggcga ttgtcgctgg tggcgaaatt gagcgagcgg
    19261 gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc gtgttgcggg
    19321 tagattgcac cgttggtcag caacgtatcg ccaaaccagc ccacggcggc ggtcatcaca
    19381 tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac gatcaccgga
    19441 acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc atcttcaatc
    19501 accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg ttgatgtgga
    19561 tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg cttgccacct
    19621 ggcggaataa ttccctgtgg gccgccgtcg acttcacccg gtacttccag cccgtgccag
    19681 tgcaacgtcg tctcttccgt cagttggtta tagatatcaa ccgttaccgc tttgccgcgc
    19741 tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt tgcagttttc
    19801 tcgccaaagg tagactgacc tgcgccaata gttaactgaa tgcgattacg agcatcggtc
    19861 gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc acggctccac
    19921 agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc acgacgttgc
    19981 atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc atagtccttc
    20041 cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt cgtcgcctcg
    20101 ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg aagacgtttt
    20161 tcagaacagt tttattcggc agcctgatgg ccgtttgcgc aaacagttac gcgctcagcg
    20221 agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg aagaacgatt
    20281 gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt ttcgcccagc
    20341 aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagccctc ggtgaagaca
    20401 gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaagtgcaaa gccctggctc
    20461 gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt tgtagaaata
    20521 ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt tacgggtgtt
    20581 aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg ctacacgacc
    20641 agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc gatcaccagg
    20701 atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat ggcgtagtac
    20761 aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt ccgctactgg
    20821 cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc atgctgcgtg
    20881 aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat gcgggtgtcg
    20941 tatcgttctg ccgtcagtat ctacccaacc ataacgccgg tagctacgac gatccgcgct
    21001 ttaagctggt gatcgatgat ggcgtcaatt tcgttaatca aaccagccag acctttgatg
    21061 tcattatctc cgactgcacc gatcctatcg gtcccggcga aagccttttc acttcggcat
    21121 tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca caaaacggcg
    21181 tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc cattacttca
    21241 gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc atgacttttg
    21301 catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt caggcgcgtt
    21361 ttctcgcctc tggcctgaaa tgccgttatt acaatccggc aatccatacg gcagcttttg
    21421 ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga gaagataaga
    21481 aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg agtttttgta
    21541 tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt gcttatatcg
    21601 atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt tccattatcg
    21661 gggctaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc agcgtcacta
    21721 ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa caccccggcc
    21781 cactgccaga aacggtcgtt gcccatcttg ataaaagtca tatttgcgta catacctacc
    21841 cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa gtctctacct
    21901 gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt gagtccgata
    21961 tcgtaaccat tgattatcgc gtgcgcggtt ttacccgcga cattaacggt atgaagcact
    22021 ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg aaggcgctgt
    22081 atgacatggt ggatgtgaac gtctatcagg aaaatatctt ccataccaag atgttgctta
    22141 aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagactta accgacagcg
    22201 agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat tacgggcgca
    22261 atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat ttatttactt
    22321 ctgctgcacg aaattacggt aagccgccac gacctgcaga aaatcctcaa cgccgcatag
    22381 cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc cagcgaattc
    22441 cagttgattg gcgcgaacca tcacctcttc accgtccatc cacagggtgt attcatgccc
    22501 tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt ccacttcatc
    22561 aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca cttcatgccc
    22621 catggacata cgcaccttta ccactccggt ggtatcgcgc agaaattcgt aatccatagt
    22681 gtgttcctct gctcccggca atgcgccgaa atcgcattgc gccattgatg taattatcgc
    22741 agccttaccg gagaaaaaca gcggaacggc gatggtttag aaataaaaaa tgcccgggaa
    22801 tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgctgactt tttaaactgc
    22861 agtctggaaa atcacaccat cggctttttc agtgtactga gaaagctggt tgaagttcag
    22921 gtaacggtaa gtatcaacgg cagttttatc cacctgcgcc acgtaggtct ggtactcttc
    22981 cggcgtcggt agtttgccaa tcagcgccgc aacagcagcc agttccgcag aagccaggaa
    23041 gacattcgcg ccagtcccca gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt
    23101 tgcaccgtcc gccacacgcg cctggttacc catacacagg gaacagccag ggatctcgat
    23161 acgcgcacca ctcttaccga agacgctgta gtagccttct tcggtcaact gtgcggcgtc
    23221 catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tggcctttat gcgcatccag
    23281 cagtttaccc gcagcacgga agtgaccgat gttggtcatg caggaaccga taaacacttc
    23341 gtcgatcttc tcaccctgta ccgcagacag cggacgcgcg tcatccgggt cgttcggagc
    23401 acacaggatt ggctctttaa tatccgccag atcgatgtcg atcactgccg cgtattccgc
    23461 atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc cctgaatacg
    23521 acgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca gcaggacgat
    23581 attagagttc aggtattcga tgatcggttc tttgttcagc ttgatggtac aaccggcagc
    23641 agaacgctcg gcggacgcat cggtcagttc aaatgcctgc tcaactttca gatccggcag
    23701 accttcaatt tccagaatgc gacctgagaa gatgtttttc ttgcctttct tctcaacggt
    23761 cagcagacct tgtttgatcg catacagcgg aatagcgtgt accagatcgc gcagggtgat
    23821 gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat ccagcggcat
    23881 tacgcctgtt gcggctgcaa atgccaccag accagaaccc gccggaaaag agataccgat
    23941 cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca gcatacggtt
    24001 cagccaggag tggattacgc cgtcacccgg acgcagcgac acaccgccac ggttcataat
    24061 gaagtccggc agcgtgtggt gcgtgttcac gtcaaccggc ttcggatacg ccgctgtgtg
    24121 gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt ctttcagttc
    24181 atcacgggtc atcgggccgg tggtgtcctg ggaacctaca gaagtcattt tcggttcaca
    24241 gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt tctgcgccag
    24301 cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat cactgtgcgg
    24361 cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca gcggaatacg
    24421 gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg tcgccagcag
    24481 ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa tcacgtcgcc
    24541 catgttcagg ttagagacgt cgacttcgat tggcagtgca cccgcgtctt ccatcgtgtt
    24601 aaagaagatt ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt tgttcggcac
    24661 atgtggaata tcatcgccca taaaccacag aacggagttg gtggcggatt tacgcgatga
    24721 acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc
    24781 aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt cacgggcgtt
    24841 tttcagcatc gccagcgcgt gcagtgggat atccgggcgc gaccacgcat ccggtgccgg
    24901 agaaaggtca tcggtgttgg tttcgccagt gactttgaag acggtaacgg tcagtttttc
    24961 agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca ttacctgctt
    25021 cgcatattcg ttgcctgctt tggctttctc ttctacgtca tagaagttat cgaacatcag
    25081 cagcgtgtga gaaagtgctt tggcagcgat cggtgccagt ttggcatcat ccagcgcgtc
    25141 gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga tggctttttc
    25201 cggagtcagc agtggggatt tggcttcacc tttcgcgaca gcagccagga agcctgcttt
    25261 gacataggcg gcttcatcga cgccaggggg aacacggttg gttaatagat ctaacaggaa
    25321 ttctttttcg cccgcgggcg ggtttttcag cagctctaca agtgcggcca tttggtttgc
    25381 atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct tacggtattc
    25441 ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga ttctcttcac
    25501 gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg cagaatagca
    25561 ggattttgac gatgtgttaa tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg
    25621 ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg catagtgata
    25681 atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc tctttatact
    25741 cagatttaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct tgagatccga
    25801 attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg cagtgccggg
    25861 ctttgtgccg cctctgccgg gctatatata aaaagccgca cagtggaagc gcctgtggaa
    25921 ccgcaatcga cacaacagac tgcgcctgac atcaccgcag ttacgcttcc tgcaacggtt
    25981 tccgcaccgc ccgtaacgcc cgccgtcgtt aaatccgcat tcagcactgc acaaatcgat
    26041 caatgggttg cacctgtcgc gctatacccc gactctctgc tttcacaagt gttaatggca
    26101 tcaacctatc cggcaaacgt tgctcaagca gtgcaatggt cgcacgataa tccacttaaa
    26161 caaggcgatg ctgctattct ggcggtatct gaccagccgt gggacgctag cgtcaaatca
    26221 ctggtggcct ttccacaatt gatggcgttg atgggcgaaa acccgcaatg ggtgcaaaac
    26281 ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca gcgattgcgg
    26341 caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt tattaccaca
    26401 acgaagaaaa ccgtaccggt aacacagaca gtcacggctc ccgtcatacc atccaatacc
    26461 gtttcaactg ccaaccccgt cattacagag cctgcaacaa ccgtcatttc cattgagccc
    26521 gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta cgggaactgg
    26581 gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc gtttgttgac
    26641 agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc actattcagc
    26701 agcatcgact gggatgacga cgatcatgac catcatcatg acgatgatta tcatcaccac
    26761 gatggcggtc atcgtgacgg taatggctgg caacacaacg gcgacaacat caatatcgac
    26821 gtcaacaatt tcaaccgtat caccggtgag catcttactg ataagaatat ggcatggcgg
    26881 cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc aaagcggttt
    26941 catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc aacacgcgat
    27001 agccagcgtc aggcggcagc aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt
    27061 acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga acactatggg
    27121 agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca acaaaaggac
    27181 gccgctcgtc agcgttatca gtcagcttct cctgagccgc gccaggcagt tcgcgagaga
    27241 atgcagacta acccgcagaa ccagcagcga agagaggcag cgcgtgagcg cattcagccc
    27301 gcctcgcctg agcagcgcca ggcagtccgc gagaaaatgc agactaaccc acagatccag
    27361 cagcgaagag aggcagcgcg tgagcgtatt cagtcagcct cgcctgagca gcgccaggtg
    27421 tttaaggaaa aagtacagca gagcccactg aaccaacagc aacgtgataa cgcccgccag
    27481 cgtgttcaat cggcatcacc tgaacaacgt caggtttttc gggagaaagt taaggagagc
    27541 cacccacaac gtctaaacga cagtaaccat actgccaggc taaataacga gcaacggtca
    27601 gcagtacgcg aacgtctctc tgagcgcgga gcaaggcgac tggaaaggta aattacagac
    27661 gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaaa
    27721 attacttctt cttcgctttc gggttcggca ggtcggtaat gctaccttcg aacacttctg
    27781 ccgccaggcc cacagactcg tgcagagtcg ggtgcgcatg gatggtcagc gcgatgtctt
    27841 cagcgtcaca acccatttcg attgccaggc cgatttcacc cagcagctcg ccgccgttgg
    27901 taccgacaat cgcaccaccg atcacacggt gagattcttt gtcgaaaatc agcttggtca
    27961 taccgtctgc gcagtcggaa gcgatagcac gaccagaagc agcccacggg aaggtggcgg
    28021 tttcatagct gatgcctttc tctttcgctt ctttctcagt cagacctacc catgcaactt
    28081 ctggttcggt ataggcgatg gacgggataa ctttcggatc gaagtagtgt ttcttaccgg
    28141 cgataacttc agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc atcggctgac
    28201 cgacgatatc gccgatagca aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc
    28261 ggatgaaacc acgatcgtca acttccacgc cagctttgcc tgcgtcgagg tttttaccgt
    28321 tcggcacacg accaatcgct accagcactg cgtcgtaacg ctgcggttca gctggtgctt
    28381 ttttgccttc catcgtcaca taaataccgt cttctttcgc ttcaacggcg gtaactttgg
    28441 tttccagcat caggttgaat ttcttgctga tacgcttggt gaagacttta acgatgtctt
    28501 tgtcagctgc cgggataacc tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg
    28561 cgtggtaaac ggtgcccatt tccagaccga tgataccgcc acccattacc agcaggcgtt
    28621 ctggtacttc tttcagttcc agcgcgtcag tggagtccca gatacgcgga tcttcatgcg
    28681 gaataaacgg cagttggatc gggcgagaac ccgctgcaat gatcgcgttg tcgaagttga
    28741 tcacggtttt accgttctca ccttcaactt ccagggtgtt agccccggta aatttaccca
    28801 gaccgttgac cactttgact ttgcggcctt tcgccatacc agccagacca ccggtcagct
    28861 gattgatcac tttctctttc caggtacgaa tcttgtcgat atcggttttc ggttcgccga
    28921 agacgatacc gtgttcagcc agcgctttgg cttcttcgat aacttttgct acgtgcagca
    28981 gtgctttaga agggatacag ccgacgttca ggcaaacacc gccaagggtg ttgtaacgtt
    29041 ctacgattac ggtttccaga cctaaatcag cgcaacggaa ggcagcggag taacctgcgg
    29101 ggcctgcccc aagtaccacg acctgagttt tgatttcagt actcatcatg acctctatat
    29161 atttatctcc ggcgggtctg acgttttttt tatgacgccc attatccacc gggtcgttct
    29221 atccgtcggt attttacaaa attgttaaca atttttaaac aacaaacggc aaccgatttg
    29281 tctattcgct aataacctca atacattcat gagattacca gaaaaaagcc ggccgttggg
    29341 ccggctcttt tacttacatc accagacggc gaatgtcaga cagcgtgttg ttaatgatgg
    29401 taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg gtcgaaggag agagaaatcg
    29461 gcagcatcag acgcggcacg aactctttac cattccacac cggctccatc gcggacttgg
    29521 aaacgccgag gatagccact tccggcgcgt tcacaatcgg cgcgaagtgg gtagtaccca
    29581 ggccgccgat gctggagatg gtgaagcaac cgccctgcat ttcgcccgca gtcagcttac
    29641 cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga cagctcgatg atgcctttct
    29701 tgttgacgtc tttgaatacc ggaacaacca aaccgttcgg ggtatccacc gccacaccga
    29761 tgttgatgta tttcttcagg gtcagacgct gaccgtcttc cgacagcgaa ctgttgaagc
    29821 gaggcatctg ctcaagagct gcagcaacgg ctttcatgat gaagacaacc ggggtgatct
    29881 tcacatccag cttacgtttc gccgcttctt cgttctgctg tttacggaac gcttccagct
    29941 cggtgatatc ggttttgtcg aagtgagtaa catgcgggat catcacccag ttacggctca
    30001 ggttcgcacc agaaattttc tggatacggc ccagttccac ttcttcgatt tcaccaaact
    30061 tgctgaagtc caccttcggc cacggcagca tgcctgggat accgccgcca gtcgccgccg
    30121 gagctgcttc tgcacgtttg atagcttctt tcacgtaagc ctgaacgtct tcgcgcagga
    30181 tacgaccttt acggccagtg cccttcactt tcgccaggtt aacgccaaac tcgcgtgcca
    30241 gacggcggat cagcggagtc gcgtgaacat aagcgtcgtt ttcagcaaat tcagatttgc
    30301 cttccgcttt cgcagctggt gctgctgccg gggcttcagc ttttgctgcc ggagccggcg
    30361 ctgccgcttc ctgtttcgca ggagctgccg caggcgctgc gccttcaact tcgaagatca
    30421 taatcagcga gccagttttc actttatcgc caacgttgac tttcagttcc ttcacgacgc
    30481 ctgcaaacgg agccggaact tccatagaag ctttgtcgcc ttctacggta atcagtgact
    30541 gttcagcggc aactttgtcg cccactttca ccatcacttc ggtcacttca acttcgtcac
    30601 cgccgatatc cggaacgtta acttctttca cgccagctgc tggtgcaggg gccgctgccg
    30661 gagccgcttc ctgtttagcc gccggagctg ccgcgcctgc ttcacccgcg acttcgaaga
    30721 ccataatcag cgagccggta gacactttgt cgccggtgtt cactttgatt tctttcacgg
    30781 tgcctgcaaa cggagccgga acttccatag aagctttatc gccttctacg gtgatcagcg
    30841 actgctcagc ttcaactttg tcgccaactt tcaccaggat ttcggtcact tcaacttcgt
    30901 cgctgccgat atccggaacg ttaacgtctt ttgccgcggc agccgcgggt gctgctgccg
    30961 gagctgcttc tttcttctct tctgcctgag caggtgcagc gtctgctgca ccgtcggcgg
    31021 aatcgaaaat cataatcagt gcgccggtct gggttttatc gccaacagag actttgatct
    31081 ctttaacgat acccgcctgc ggagacggaa cttccataga ggctttgtcg ccttctacgg
    31141 tgatcagcga ctgttcggct tcaactttgt cgcccacttt gaccaggatc tcggtgattt
    31201 caacttcatc agccccgatg tccggtactt tgatttcgat agccattatt cttttacctc
    31261 ttacgccaga cgcgggttaa ctttatctgc atcgatgttg aatttggcga ttgcgtcagc
    31321 aaccactttc ttatcgattt cgccacgttt agccagttcg cccagcgccg caaccacgac
    31381 ataagaagca tcaacttcga agtggtgacg caggttctca cggctgtcgg aacgaccgaa
    31441 gccatcagta cccagtacgc ggtagtcgtc agccggtacg taagtacgga cctgctcagc
    31501 gaacagtttc atatagtcgg tagatgccac tgccggagcg tcgttcatca cctgcgcgat
    31561 atacggaacg cgcggagttt ccagtgggtg cagcatgttc cagcgttcac aatcctgacc
    31621 atcacgcgcc agctcggtga aggaggtcac gctataaacg tcagaaccta cgccgtaatc
    31681 tttcgccagg atctctgccg cttcacggac gtgacgcagg atagaaccgg agcccagcag
    31741 ctgaacttta cctttgctac cttcaatggt ttcgagtttg tagatacctt tacggatacc
    31801 ttcctcagca ccttccggca ttgccggcat gtggtagttt tcgttcagcg tggtgatgta
    31861 gtagtaaacg ttctcttgtt tttcaccgta catacgctcc agaccgtcat gcataatgac
    31921 agcaacttcg taagcgtaag ccgggtcgta agagatacag ttcggaatag tcagcgactg
    31981 aatatggctg tgaccatctt cgtgctgcag accttcaccg ttcagggtgg tacgaccgga
    32041 agtaccgccg atcaggaagc cacgcgcttg ctggtcgcca gccgcccagc acagatcgcc
    32101 gatacgctgg aagccgaaca tcgagtaata gatgtagaac ggaatcatcg gcagattgtt
    32161 ggtgctgtaa gaggtcgccg ctgccagcca ggaacaacct gcgcccagct cgttgatccc
    32221 ttcctgcaga atctgacctt tctcgtcttc tttatagtaa gcaacctgct cgcggtcctg
    32281 cggggtgtac tgctgaccgt tcgggctgta aataccaatc tgacggaaca gaccttccat
    32341 accgaaagta cgcgcttcgt cggcgatgat cggtaccaga cgatctttga tcgacttgtt
    32401 cttcagcatc acgttcaggg cacgaacgaa agcgatagtg gtagagatct ctttgctttg
    32461 ctcttccagc agcgcgccga agtcttgcag gctcggcagc tcaagcttct cggtgaagtt
    32521 cggctgacgg cttggcagat aaccgtgcag tttctgacgc tgagcgtgca gataggtatg
    32581 ctcttcagaa ccttccggga aggtgatgta cggcagtttt tcgatatctg catcagacac
    32641 cggcacattg aaacggtcgc ggatatgacg cacaccgtcc atgttcattt tcttaacctg
    32701 gtgcgcgatg tttttacctt cagccgcgtc gcccatgccg taacctttaa tggtatgagc
    32761 aaggattact gtcgctttgc ctttggtttc ctgcgctttc ttgaatgcag cgtagatttt
    32821 cttcggatcg tgaccaccac ggttcagtgc ccagatctgc tcgtcagtcc agtctgcaac
    32881 cagtgctgcg gtttcaggat atttaccgaa gaagtgttca cgaacgtacg caccatcttt
    32941 cgatttgaag gtctggtagt cgccgtcaac ggtttcgttc atcagctgga tcagtttacc
    33001 gctggtatct ttacgcagca gttcatccca acggctaccc cacatcactt tgatcacgtt
    33061 ccagccagca ccttcgaaga tgccttccag ttcgttgatg atcttgccgt taccggtgac
    33121 cgggccgtca agacgctgca ggttacagtt gataacgaag accaggttat ccagtttttc
    33181 acgggtagcg atggtgatcg cacctttgga ttccggttcg tccatttcac cgtcgccgag
    33241 gaacgcgtaa acggtctgtt tagaggtatc tttcaggcca cggtgttcca gatatttcag
    33301 gaatttagcc tggtaaatag caccaatcgg acccagaccc atagatacgg tcgggaactg
    33361 ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag aggccattgc cgtgaacttc
    33421 ctgacggaag ttatccagct gctcctgagt cagacgacct tccaggaaag cacgagcgta
    33481 aacgcccggg gagatgtggc cctggaagta aaccaagtcg ccgccatcct gctcgttgcg
    33541 tgcacggaag aagtggttaa agcacacatc ataaatggtt gcggaagact ggaaggacgc
    33601 catgtggccg cccagttcga ggtctttttt cgacgcacgc agaaccgtca tgatggcgtt
    33661 ccagcggata gctgaacgaa tacggcgttc cagttccaga ttacccggat actccggttg
    33721 ttcttcaacg gggatggtgt tgatgtagtt gctgataccg gtgcctgcgg ctacgttgac
    33781 accgcctttg cgggcttcag caagcagttg gtcgatcaga tactgagcac gctcaacacc
    33841 ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg cgagtttcga tcggatccac
    33901 gtcatttggg aaacgttctg acatgggtta ttccttatct atctaataac gttgagtttt
    33961 ctggaacctg tcccattgaa ctctcgccgg aaagctcaat aagacaggtt ctacgtttag
    34021 ttgccgcgct tttatatgcg cttgatttac aacatcttct ggataatttt taccagaaaa
    34081 atcactaatt ctttcgttgc tccagacgac gcagagaacg ctcacggcgg ctctcttcac
    34141 ggcttctgtc gagcaaaatt tcttcgataa aggccagatg gcgatgcgat gcttcgcgcg
    34201 cttcttccgg cttaccggcc ataattgctt cgaatatacg ggtgcggtga ctactcacca
    34261 gcggcagcat ctcgcgacgc gcatagagca attcgaagtt ctgacggaca ttctgggcca
    34321 acatcggctc catacacctt agcagatgaa gcaaaaccac attgtgggct gcttcggtga
    34381 cggcaatctg atactggagt acggcgtttg attccgcgtc cagatcgcca gactgctgcg
    34441 ccagttctat ggcgtggtgg agttcacgga tgcgttcctt gtcttcatcg gtactacgca
    34501 gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg tgtttcgagc aagtcatact
    34561 gtgattcagg atggtcggag agcagctcta ccagcggatc gctgaagctt tgccataggc
    34621 tgctctggac aaaagtgccg ccaccctggc gacgaagcaa caagcccttc gcttcgagac
    34681 gttgaatcgc ctcacgcaag gagggacggg agacgtcaaa ctgttttgcc agttcgcgtt
    34741 ccggtgggag tttttcgccc gggcggagag tgccttcgag gatcaaaaac tccagttgct
    34801 gctcaatcac atcggagagt tttggttggc ggattttgct gtaggccatg agttcctgtc
    34861 ttaagccact tgccgaagtc aattggtctt accaatttca tgtctgtgac gctaaagtaa
    34921 caaagtattc accttatgtc catacaggtt ttgattgaaa tcatgaaact gtgcacattt
    34981 taacaacttg acatatataa cgtttcaaag ttgtaactat gcacaaatgt agactttacg
    35041 t
//
LOCUS       DAZKFU020000016        34683 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZKFU020000016 DAZKFU020000000
VERSION     DAZKFU020000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.016"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..1295
                     /gene="uidC"
                     /locus_tag="ACHOQQ_RS03200"
                     /old_locus_tag="pgaptmp_000640"
     CDS             30..1295
                     /gene="uidC"
                     /locus_tag="ACHOQQ_RS03200"
                     /old_locus_tag="pgaptmp_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HGU4468938.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(1340..2848)
                     /gene="ydgA"
                     /locus_tag="ACHOQQ_RS03205"
                     /old_locus_tag="pgaptmp_000641"
     CDS             complement(1340..2848)
                     /gene="ydgA"
                     /locus_tag="ACHOQQ_RS03205"
                     /old_locus_tag="pgaptmp_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4468939.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEEMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(2949..4124)
                     /gene="manA"
                     /locus_tag="ACHOQQ_RS03210"
                     /old_locus_tag="pgaptmp_000642"
     CDS             complement(2949..4124)
                     /gene="manA"
                     /locus_tag="ACHOQQ_RS03210"
                     /old_locus_tag="pgaptmp_000642"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGU4468940.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIL
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            4323..5969
                     /gene="fumB"
                     /locus_tag="ACHOQQ_RS03215"
                     /old_locus_tag="pgaptmp_000643"
     CDS             4323..5969
                     /gene="fumB"
                     /locus_tag="ACHOQQ_RS03215"
                     /old_locus_tag="pgaptmp_000643"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4468941.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            6112..7515
                     /gene="fumC"
                     /locus_tag="ACHOQQ_RS03220"
                     /old_locus_tag="pgaptmp_000644"
     CDS             6112..7515
                     /gene="fumC"
                     /locus_tag="ACHOQQ_RS03220"
                     /old_locus_tag="pgaptmp_000644"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGU4468942.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(7512..8441)
                     /gene="tus"
                     /locus_tag="ACHOQQ_RS03225"
                     /old_locus_tag="pgaptmp_000645"
     CDS             complement(7512..8441)
                     /gene="tus"
                     /locus_tag="ACHOQQ_RS03225"
                     /old_locus_tag="pgaptmp_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGU4468943.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHNPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(8517..9818)
                     /gene="rstB"
                     /locus_tag="ACHOQQ_RS03230"
                     /old_locus_tag="pgaptmp_000646"
     CDS             complement(8517..9818)
                     /gene="rstB"
                     /locus_tag="ACHOQQ_RS03230"
                     /old_locus_tag="pgaptmp_000646"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGU4468944.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(9822..10541)
                     /gene="rstA"
                     /locus_tag="ACHOQQ_RS03235"
                     /old_locus_tag="pgaptmp_000647"
     CDS             complement(9822..10541)
                     /gene="rstA"
                     /locus_tag="ACHOQQ_RS03235"
                     /old_locus_tag="pgaptmp_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGU4468945.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            10670..11005
                     /gene="ydgC"
                     /locus_tag="ACHOQQ_RS03240"
                     /old_locus_tag="pgaptmp_000648"
     CDS             10670..11005
                     /gene="ydgC"
                     /locus_tag="ACHOQQ_RS03240"
                     /old_locus_tag="pgaptmp_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGU4468946.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(11002..11724)
                     /gene="folM"
                     /locus_tag="ACHOQQ_RS03245"
                     /old_locus_tag="pgaptmp_000649"
     CDS             complement(11002..11724)
                     /gene="folM"
                     /locus_tag="ACHOQQ_RS03245"
                     /old_locus_tag="pgaptmp_000649"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGU4468947.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(11761..13143)
                     /gene="ydgI"
                     /locus_tag="ACHOQQ_RS03250"
                     /old_locus_tag="pgaptmp_000650"
     CDS             complement(11761..13143)
                     /gene="ydgI"
                     /locus_tag="ACHOQQ_RS03250"
                     /old_locus_tag="pgaptmp_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4468948.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(13329..14273)
                     /gene="ydgH"
                     /locus_tag="ACHOQQ_RS03255"
                     /old_locus_tag="pgaptmp_000651"
     CDS             complement(13329..14273)
                     /gene="ydgH"
                     /locus_tag="ACHOQQ_RS03255"
                     /old_locus_tag="pgaptmp_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGU4468949.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            14797..16329
                     /gene="pntA"
                     /locus_tag="ACHOQQ_RS03260"
                     /old_locus_tag="pgaptmp_000652"
     CDS             14797..16329
                     /gene="pntA"
                     /locus_tag="ACHOQQ_RS03260"
                     /old_locus_tag="pgaptmp_000652"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGU4468950.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            16340..17728
                     /gene="pntB"
                     /locus_tag="ACHOQQ_RS03265"
                     /old_locus_tag="pgaptmp_000653"
     CDS             16340..17728
                     /gene="pntB"
                     /locus_tag="ACHOQQ_RS03265"
                     /old_locus_tag="pgaptmp_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGU4468951.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(17753..18787)
                     /gene="tqsA"
                     /locus_tag="ACHOQQ_RS03270"
                     /old_locus_tag="pgaptmp_000654"
     CDS             complement(17753..18787)
                     /gene="tqsA"
                     /locus_tag="ACHOQQ_RS03270"
                     /old_locus_tag="pgaptmp_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HGU4468952.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            19199..19564
                     /gene="mdtJ"
                     /locus_tag="ACHOQQ_RS03275"
                     /old_locus_tag="pgaptmp_000655"
     CDS             19199..19564
                     /gene="mdtJ"
                     /locus_tag="ACHOQQ_RS03275"
                     /old_locus_tag="pgaptmp_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGU4468953.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            19551..19880
                     /gene="mdtI"
                     /locus_tag="ACHOQQ_RS03280"
                     /old_locus_tag="pgaptmp_000656"
     CDS             19551..19880
                     /gene="mdtI"
                     /locus_tag="ACHOQQ_RS03280"
                     /old_locus_tag="pgaptmp_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGU4468954.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(19919..20740)
                     /gene="ydgD"
                     /locus_tag="ACHOQQ_RS03285"
                     /old_locus_tag="pgaptmp_000657"
     CDS             complement(19919..20740)
                     /gene="ydgD"
                     /locus_tag="ACHOQQ_RS03285"
                     /old_locus_tag="pgaptmp_000657"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGU4468955.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(20840..20923)
                     /locus_tag="ACHOQQ_RS03290"
                     /old_locus_tag="pgaptmp_000658"
     CDS             complement(20840..20923)
                     /locus_tag="ACHOQQ_RS03290"
                     /old_locus_tag="pgaptmp_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4468956.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(21016..21324)
                     /gene="asr"
                     /locus_tag="ACHOQQ_RS03295"
                     /old_locus_tag="pgaptmp_000659"
     CDS             complement(21016..21324)
                     /gene="asr"
                     /locus_tag="ACHOQQ_RS03295"
                     /old_locus_tag="pgaptmp_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGU4468957.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            21595..21699
                     /locus_tag="ACHOQQ_RS03300"
                     /old_locus_tag="pgaptmp_000660"
     CDS             21595..21699
                     /locus_tag="ACHOQQ_RS03300"
                     /old_locus_tag="pgaptmp_000660"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGU4468958.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(21748..23001)
                     /gene="ynfM"
                     /locus_tag="ACHOQQ_RS03305"
                     /old_locus_tag="pgaptmp_000661"
     CDS             complement(21748..23001)
                     /gene="ynfM"
                     /locus_tag="ACHOQQ_RS03305"
                     /old_locus_tag="pgaptmp_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4468959.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            23108..24001
                     /gene="ynfL"
                     /locus_tag="ACHOQQ_RS03310"
                     /old_locus_tag="pgaptmp_000662"
     CDS             23108..24001
                     /gene="ynfL"
                     /locus_tag="ACHOQQ_RS03310"
                     /old_locus_tag="pgaptmp_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4468960.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            24136..25356
                     /gene="mlc"
                     /locus_tag="ACHOQQ_RS03315"
                     /old_locus_tag="pgaptmp_000663"
                     /gene_synonym="dgsA"
     CDS             24136..25356
                     /gene="mlc"
                     /locus_tag="ACHOQQ_RS03315"
                     /old_locus_tag="pgaptmp_000663"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGU4468961.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AVYNGSLLIRLLQG"
     gene            25481..26176
                     /gene="bioD"
                     /locus_tag="ACHOQQ_RS03320"
                     /old_locus_tag="pgaptmp_000664"
     CDS             25481..26176
                     /gene="bioD"
                     /locus_tag="ACHOQQ_RS03320"
                     /old_locus_tag="pgaptmp_000664"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4468962.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(26129..27421)
                     /gene="clcB"
                     /locus_tag="ACHOQQ_RS03325"
                     /old_locus_tag="pgaptmp_000665"
     CDS             complement(26129..27421)
                     /gene="clcB"
                     /locus_tag="ACHOQQ_RS03325"
                     /old_locus_tag="pgaptmp_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGU4468963.1"
                     /translation="MHHLHIYPNLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(27582..28196)
                     /gene="dmsD"
                     /locus_tag="ACHOQQ_RS03330"
                     /old_locus_tag="pgaptmp_000666"
     CDS             complement(27582..28196)
                     /gene="dmsD"
                     /locus_tag="ACHOQQ_RS03330"
                     /old_locus_tag="pgaptmp_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGU4468964.1"
                     /translation="MTHFSQQDNFSVVARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGKLARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(28239..29093)
                     /gene="ynfH"
                     /locus_tag="ACHOQQ_RS03335"
                     /old_locus_tag="pgaptmp_000667"
     CDS             complement(28239..29093)
                     /gene="ynfH"
                     /locus_tag="ACHOQQ_RS03335"
                     /old_locus_tag="pgaptmp_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGU4468965.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(29095..29712)
                     /gene="ynfG"
                     /locus_tag="ACHOQQ_RS03340"
                     /old_locus_tag="pgaptmp_000668"
     CDS             complement(29095..29712)
                     /gene="ynfG"
                     /locus_tag="ACHOQQ_RS03340"
                     /old_locus_tag="pgaptmp_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGU4468966.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(29723..32146)
                     /gene="ynfF"
                     /locus_tag="ACHOQQ_RS03345"
                     /old_locus_tag="pgaptmp_000669"
     CDS             complement(29723..32146)
                     /gene="ynfF"
                     /locus_tag="ACHOQQ_RS03345"
                     /old_locus_tag="pgaptmp_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HGU4468967.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPKMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(32207..34633)
                     /gene="ynfE"
                     /locus_tag="ACHOQQ_RS03350"
                     /old_locus_tag="pgaptmp_000670"
     CDS             complement(32207..34633)
                     /gene="ynfE"
                     /locus_tag="ACHOQQ_RS03350"
                     /old_locus_tag="pgaptmp_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HGU4468968.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
BASE COUNT         8677 a         8543 c         8945 g         8518 t
ORIGIN      
        1 aaaataatca gaacatcaaa ggtgcaacta tgagaaaaat agtggccatg gccgttattt
       61 gcctgacggc tgcctctggc cttacctctg cttacgcggc gcaactggct gacgatgaag
      121 cgggactacg catcagactg aaaaacgaat tgcgcagggc tgataagccc agtgctggcg
      181 cgggaagaga tatttacgca tgggtacagg gaggattgct cgatttcaat agtggttatt
      241 attccaatat tattggcgtt gaaggcggag cgtattatgt ttataaatta ggtgctcgtg
      301 ctgatatgag tacccggtgg tatcttgatg gtgataaaag ttttggcttt gccctggggg
      361 cagtaaaaat aaaacccagt gaaaatagcc tgcttaaatt aggtcgcttc gggacggatt
      421 atagttatgg tagcttacct tatcgtattc cgttaatggc tggcagttcg caacgtacat
      481 taccgacagt ttctgaagga gcattaggtt attgggcttt aacgccaaat attgatctgt
      541 ggggaatgtg gcgttcacga gtatttttat ggactgattc aacaaccggt attcgtgatg
      601 aaggggtgta taacagccag acgggaaaat acgataaaca tcgcgcacgt tcttttttag
      661 ccgccagttg gcatgatgat accagtcgct attctctggg ggcatcggta cagaaagatg
      721 tttccaatca gatacaaagt attctcgaga aaagcatacc gctcgacccg aattatacgt
      781 tgaaagggga gttgctcggc ttttacgcgc agctcgaagg tttaagtcgt aataccagcc
      841 agcccaatga aacggcgttg gttagtggac aattgacctg gaatgcgccg tggggaagcg
      901 tatttggcag tggtggttat ttgcgccatg caatgaatgg tgccgtggtg gataccgaca
      961 ttggctatcc cttttcatta agtcttgatc gtaaccgtga aggaatgcag tcctggcaat
     1021 tgggcgtcaa ctatcgttta accccgcaat ttacgctgac atttgcaccg attgtgactc
     1081 gcggctatga atccagtaaa cgagatgtgc ggattgaagg cgcgggtatc ttaggtggta
     1141 tgaactatcg ggtcagcgaa gggccgttac aggggatgaa tttctttctt gctgccgata
     1201 aagggcggga aaagcgcgat ggcagtacgc tgggcgatcg cctgaattac tgggatgtga
     1261 aaatgagtat tcagtatgac tttatgctga agtaagaaat aacgccggag agaaaaacct
     1321 ccggcgtttt ggctgtgaat tactgctgcg gaatagcggg tacgaccgga acgtttaatg
     1381 ccggcattgc aaacatacca acgaaatctt ccagtggcat tttctgccca tttaacgtta
     1441 tctgaccgtt agcatattgc aggctggtgg tgatggtatt gtcctgcaag gtggtcagac
     1501 ggaacatctg ccccattgct gatgcacctt caacttgctg tttcgccagt tttttcgctt
     1561 gatcttcctg ataaccttcc agcttcgcta cctgagtcat caactcagtt gccatatcca
     1621 ccggaatggt cagtttcgca tccagagatt taaccgaacg atctacttcc tgcgccagcg
     1681 tttgcggcgc ttctttagtc gttgccgggt ctttcaggaa cagcgacaga ttcagcgcac
     1741 tttcaccctg actgtttttc cagcttagcg gcgcgatagt aatcaccgga tcgcctttca
     1801 acatcagcgg cagggcgcta aagaaggctt ccgtcacttt ctcctgataa agttcgggat
     1861 tgttggcaat ttctggctgt gccagcagcg cctgagtttg cgcgttatat tgctggctaa
     1921 actgatgcca ggcttcacca tcaatctggc cgacttttaa agtcagcttg ccgctgccca
     1981 gatcctgatt ctgtaccttc aggctgttta gcgagtaatc cagttggcta ttgatcgttt
     2041 taccgtcatt gaccagatcc gatttaccgc tgatctccat gccttccagc agtgccagtt
     2101 ctttgccttc cactgaaatg gtcatttttt ccagtgacag tttttggttt cctacgcgct
     2161 caccaaaact tgccagcgtg ctggaaccgt cggttttcag attattaaag gtcaactgta
     2221 ctttctggtt gtattcgtta accgcgtcta tccgaccact ttgcgcctcc ccggaaaggg
     2281 agatagcttt gccgtctctg tccgcattta actggaactc gccgccgcta aaggcgactt
     2341 tttcatcctt ttgctcgtaa ttcagcggct tgagcgaaat atcggaactg gaatcaccgc
     2401 tgtaaccaat gcgcgagtta atctcaaaag gcgtttcacc ttttgccata tcaaacagtg
     2461 gtttgcttac ttcgttatta accagcgtgg tttgaattga tgccatcgat gggatcaggt
     2521 tcagtttttt aagctgggca agggggaagg gaccatgatc aaccgattcg ttgaagatga
     2581 cgctctgacc gcttttaatc cacggatttt ctttcccggc aatgggtttc accaacagtt
     2641 gcaactggct gctgaatacg ccgcgatgat agttttgata actcacttcc aggttggatt
     2701 caggagctgt cagtttgagt tgcgcgttcg cctgcgcgac catctcttcg agatgggttt
     2761 caatcttctt gcctgtatac catgcgccgc ctgtccagac tacgcctagc gcaacaatga
     2821 cgcctaccgc taccagcgat ttattcataa tgattatcca taaaatgaaa tcaggcggac
     2881 tggccgcctg aaggtgttat aagcctttaa taagcttagc aagagatgtt aattttttca
     2941 gtaagctctt acagcttgtt gtaaacacgc gctaaacggc cgtggccttt gacagtcacc
     3001 ggtgattcgt tggcggcaat aaacgctgat tcacccggtt taagctgtaa ctgctgagaa
     3061 cctttccaca acgttgcatc gccttcgacg cagaacaaaa tggcggcact ctgctggcta
     3121 atggtggttt ctttatcact aaggtcatgc agcgagaagg caaaatcatc aacaggaatc
     3181 gggaagtcca gttctgcacc ttgtttcacc ggctgggtca acaactggtt agccggtttg
     3241 gcttcgaact tcacattggc aaccagttcc ggaatatcaa tgtatttagg cgtcagaccc
     3301 gcacgcagca cgttatcgga gtttgccatc acttccagcg ccacgccttg caggtaagcg
     3361 tgcggtgttt cagcgaacag gaacatcgct tcgccaggat tcaatttcac cacattcagc
     3421 aacagcggag agaacaggcc gctgtcatcc gggtaaaatt cagaaattaa aagaatcgtt
     3481 tgccacggtt caccctgctg actgtcgagc gccgatttta aaatcgccag cgcgcgggat
     3541 ttttcttcac cctgcatatt caacaggctg gcgaacagtt cgcttaaacg ttcggcatca
     3601 ggctgttgta aaaagtgagc aatcgccgga tgtgcacctg cgaccggctg gagtagggag
     3661 acaatctcgg aaaattcacg aaacgcgttc atcgcaagga aaggcgtcag cgcaaaaacc
     3721 agctcaggct tgtggttagg atctttatag ttacgctcgg cggcatccat cgggatacct
     3781 gcggcatttt ctttggcaaa accgatttca gaattgtgtt tgtttggatg aacctgaatg
     3841 gagagcggct gtgctgcgca taatactttg aacaggaaag gcagttcgcc aaagcgtttg
     3901 gcaacggcct ctccgagcag agtcgattta tcactctcaa tcacatcacg cagtgaaacg
     3961 atatctccgg cggcattctg cactcgtgaa ctgcttttcg gatgtgcgcc catccacagc
     4021 tcggccatcg gctggctgga cggattttcc ataccataaa gttcagtcaa cgccgttttg
     4081 ctgccccagg catagttttg cactgagtta atgagttttt gcatgatcaa tccctgtttt
     4141 aatgtggaaa ttaatcccgc tattaaagca agaatcctac gggaagtaac ctggagccgc
     4201 aaaaagtcgt actagtctca gtttttgtta aaaaagtgtg taggatattg ttactcgctt
     4261 ttaacagggc aacggaacac ccgcccagag cataaccaaa ccaggcagta agtgagagaa
     4321 caatgtcaaa caaacccttt cattatcagg ctccttttcc actcaaaaaa gatgatactg
     4381 agtattacct gctaaccagc gaacacgtta gcgtatctga atttgaaggg caggagattt
     4441 tgaaagtcgc acccgaagcg ttaactctgt tggcgcgtca ggcgtttcat gatgcgtcgt
     4501 ttatgctgcg tccggcgcac caacaacagg tggccgacat tctgcgtgac ccggaggcca
     4561 gcgaaaatga taaatatgtg gcgctgcaat tcctgcgtaa ctccgacatc gcggcgaaag
     4621 gcgttctgcc aacctgtcag gataccggca ccgcgattat tgttggtaaa aaagggcagc
     4681 gtgtatggac cggtggtggt gatgaagcgg cgctggcgcg cggtgtctat aacacttata
     4741 tcgaagataa tctgcgctac tcgcaaaacg cgccgctgga tatgtataaa gaagtgaata
     4801 ccggcaccaa tctaccagcg cagatcgatc tttatgccgt tgatggcgac gagtacaaat
     4861 tcctctgtat cgccaaaggt ggtggttcgg caaacaagac gtatctctat caggaaacca
     4921 aagcgttact gacgccgggg aaactgaaaa attacctggt tgagaagatg cgcacgctgg
     4981 gtacggcggc ctgtcctccg tatcatattg cgttcgttat tggtggaact tctgcagaaa
     5041 cgaaccttaa aacggtgaaa ctggcttccg cgaaatacta tgatgaactg ccaacggaag
     5101 ggaatgagca cggtcaggcg ttccgcgatg tggaactgga aaaagaattg ctgatcgaag
     5161 cgcaaaatct tggtctgggt gcgcagtttg gtggtaaata cttcgctcac gacatccgcg
     5221 tgattcgcct gccacgtcac ggcgcatcct gcccggtcgg tatgggcgtc tcctgctctg
     5281 ctgaccgtaa tatcaaagcg aagatcaacc gtcaggggat ctggatcgaa aaactggaac
     5341 ataatccagg caaatatatc ccggaagagc tgcgcaaagc gggagaaggc gaagcggtgc
     5401 gcgttgacct taaccgtccg atgaaagaga tcctcgcaca gttgtcgcag tatcccgttt
     5461 ctacacgctt atcgcttaac ggcacgatta tcgttggtcg tgatattgcc cacgccaaac
     5521 tgaaagagcg gatggataac ggtgaagggc tgccgcagta cattaaagat catccgattt
     5581 actacgcggg tccggccaaa acgccggaag gttatgcctc cggttctctt ggcccaacga
     5641 ccgccggacg gatggattct tatgtcgatc aactgcaagc gcagggcgga agtatgatca
     5701 tgctggcgaa aggcaaccgc agccagcagg tgacggatgc ctgtaaaaaa cacggcggct
     5761 tctaccttgg cagtatcggt ggtccggccg ctgtattggc gcagggaagt attaagagcc
     5821 tggaatgtgt tgaatatccg gaactgggaa tggaagccat ctggaaaatt gaagtggaag
     5881 atttcccggc gtttattctt gtggatgata aaggaaatga cttctttcag cagatacaac
     5941 tcacacaatg cacccgctgt gtgaaataaa cagagccgcc cttcggggcg gtttttttac
     6001 atggcacgaa agaccaaaca tttgttatca aatggtaaat aataagtgag ctaaaagttg
     6061 cttaacgaaa gcaaaacaga aagaaaaaat taatcaggtg aggagcaggc catgaataca
     6121 gtacgcagcg aaaaagattc gatgggggcg attgatgtcc cggcagataa gctgtggggc
     6181 gcacaaactc aacgctcgct ggagcatttc cgcatttcga cggagaaaat gcccacctca
     6241 ctgattcatg cgctggcgct aaccaagcgt gcagcggcaa aagttaatga agatttaggc
     6301 ttgttgtctg aagagaaagc gagcgccatt cgtcaggcgg cggatgaagt actggcagga
     6361 cagcatgacg acgaattccc gctggctatc tggcagaccg gctccggcac gcaaagtaac
     6421 atgaacatga acgaagtgct ggctaaccgg gccagtgaat tactcggcgg tgtgcgcggg
     6481 atggaacgta aagttcaccc taacgatgac gtgaacaaaa gccaaagttc caacgatgtc
     6541 tttccgacgg cgatgcacgt tgcggcgctg ctggcgctgc gcaagcaact cattccgcag
     6601 cttaaaaccc tgacacagac actgaatgag aaatcccgtg catttgccga tatcgtcaaa
     6661 attggtcgta ctcacttgca ggatgccacg ccgttaacgc tggggcagga gatttccggc
     6721 tgggtagcga tgctcgagca taatctcaaa catatcgaat acagcctgcc tcacgtagcg
     6781 gaactggctc ttggcggtac agcggtgggt actggactaa atacccatcc ggagtatgcg
     6841 cgtcgcgtag cagatgaact ggcagtcatt acctgtgcac cgtttgttac cgcgccgaac
     6901 aaatttgaag cgctggcgac ctgtgatgcc ctggttcagg cgcacggcgc gttgaaaggg
     6961 ttggctgcgt cactgatgaa aatcgccaat gatgtccgct ggctggcctc cggcccgcgc
     7021 tgcggaattg gtgaaatctc aatcccggaa aatgagccgg gcagctcaat catgccaggg
     7081 aaagtgaacc caacacagtg tgaggcatta accatgctct gctgtcaggt gatggggaac
     7141 gacgtggcga tcaacatggg tggcgcttcc ggtaactttg aactgaacgt cttccgtccg
     7201 atggtgatcc ataatttcct gcaatcggtg cgcttgctgg cagatggcat ggaaagtttt
     7261 aacaaacact gcgcagtggg tattgaaccg aatcgtgagc gaatcaatca attactcaat
     7321 gaatcgctga tgctggtgac tgcgcttaac acccacattg gttatgacaa agccgcggag
     7381 atcgccaaaa aagcgcataa agaagggctg accttaaaag ctgcggccct tgcgctgggg
     7441 tatcttagcg aagccgagtt tgacagctgg gtacggccag aacagatggt cggcagtatg
     7501 aaagccgggc gttaatctgc aacatacagg tgcagccgtg gaatgatcaa acgaagcggc
     7561 tgcgcctgag gtttataacg atgctgcaca ttgtcggcat cgtaatttaa caactcacca
     7621 acgtccggca cgcccgcacc attatcccga ttaatcagtg caatcagcgg tgtagggcag
     7681 gcgtgttgca cttgtttttg atctcctttg taccagacgc gggcaatcgg ctgcaccttc
     7741 accggacgtt tgatttttaa cttcgcgttc tgtggcaggg cagcgatatc ctgatactct
     7801 cgctccagtt ttctttgcca ctcctcgcgc gtccacggtg cgacactgcg tggtgatttc
     7861 aggctttttt ccagctgtgc caggacttca tcacgatgca aatttttaat gatatgttta
     7921 ttagcccagc caaaacgtaa agtggcgggg ttgtgcagaa cggtgagcgt gcggtaagca
     7981 ttaagggtga tcagccccgg caaatgacga tgcacccatt caaaacgtgc cgcggtgggg
     8041 agttctgatt caaccgtgac gatatgctcg aacgtggtct tgagtttatt gatgtgctga
     8101 atatgactga ccaacgctgc ttgcgaaagg ttatcgacct ggtaacacaa cacgccaggc
     8161 agacggacag cggctttgct gctgcgattt tcggactgtt gttgaataaa taaatgacgg
     8221 aaatgacgca acgccagcga ctgcgcgtcg ttgccgagat gttgtttcac ctcaatacga
     8281 ttaagcggat tatgctcatc ctcttttttt acctccggca aagagaacac gcgggcaacc
     8341 aatagcttgt gttgctcaag atgagcggca aatgcagcca gctcttgttc catctggcga
     8401 aaagtagtgt tgagtcggtc tacgagatcg taacgcgcca taatattaac cactttagtt
     8461 acaacatcct taatttatag cacagtggtg gtgacttgac caccgtgcgc gtagtgtcaa
     8521 gcagaggtaa attgcgggat gttatgccat aacggccagc taaacgagaa gcgggcacca
     8581 cccagttcgc tggtgtcaca attaaccgta ccgcccattg ccagtgctat agagtggaca
     8641 attgccagcc ccagcccgca gccgccggtt gagcgatccc ggctgggatc gaggcgaaca
     8701 aaaggttcaa agatatgttc gcggttttct ggggcaatcc ctgggccatc atcctcaaca
     8761 attaatgtcg ctctattccc cgacagtagc aggctggttt caaccgttga atggcagtag
     8821 cgcagggcgt tattgagcaa attatccagc acgcgctcca ttaagcgcat atccaacgcc
     8881 gcataatggc cttgcatgag cgtttttatc cgtaccgttt tatcgggcgt tactgcctga
     8941 atatctgcca gatgcgttga tagccacaac ggcaggtctg gttcgctaag atgaagctcg
     9001 ttttgtgggc gatcgagtcg ggcataagtc agcagctctt caattaaagc ttcaagttga
     9061 ctgatatcac gattcaacgc ctgggattcg gcggcgctca ggttatcgct catctccagt
     9121 cgataacgca ggcgcactaa cggtgttcgc agttcgtgag cgataccgtc aataagctgt
     9181 tttttgctgg caatcaaggc gttgatattg tccgccatct ggttaaatgc gataccaagt
     9241 cgttcaaagc tcgaaccctc atcaaagtgg atacgttcat tgagatgccc atcgccaaat
     9301 cgttgcgccg ctgcttccag ttttaacata tcctgccagt gcggacgcat ccagataaac
     9361 accggaaagg cgagggaaat agcaataaaa gcgatcaggg cgatatccag caatcgcatc
     9421 tgatggaggt aataaagata aggaacagga ccaactgcca gcacgtagtg gctgcgcggg
     9481 atacgctgca aaaacgtgta ctgatcgtcc agggcgacaa tttcgccgcc acgcagtcgg
     9541 tgcatggaaa tatcatcaag atggtattta ctcagtggct cgacacgcag atcgaaagag
     9601 agatttaaat ccatctcttt cagcgtctta ccccagtcgt gtggggggat ctcacgcaat
     9661 tcgctgcgca tcagatacag cgaactgttc atcaaatcat ccagcgactg tttgcccgcg
     9721 cgttcggcgg taaatttgta caccagccca accagcagag acattacaag gaagcagaca
     9781 aacaataata ggtaaaactg gataaacagt tttttcatcg cttattccca tgcatgaggc
     9841 gcaaagagat agcctttgtt acgcacagtt ttaatgcgat aaggttctgc ggcgttatcg
     9901 agcagttttt ttcttaaccg cgaaatagcc acgtccacgc tacgatccag gccgtcataa
     9961 ctgacgccgc gtaaattttt cagcaatgca tcgcggtcca tgatttgccc ggcatgggta
    10021 gctaattccc acaataattc gaaatcagct gtcgagagcg agatttcagt gttagccagg
    10081 gtgactacgc ggttgatggg atcgatggtc aacgtgccga aatgcagggc tttgtaggga
    10141 gtcagagacg tttcctgaag acctttggtc agtgtggctt gctcattctg acgcaaatgc
    10201 aaacgtaaac gcgctagcaa aacagcaggg ggcgtcgttt tgagaatata gtcgcaggca
    10261 cccatttcca gtgccaggat gtggttcata tcgctatcga gagaggttag aagaacaatc
    10321 ggtccagacc actttgcgcg taaatcacga caaatggtca tgccgtcctt gcctggtagc
    10381 atgatgtcga gtaacaccaa atccggattt tctcgcaaaa tggtttcttc ggcctggtcg
    10441 ccgcgcggct ctacggtaac ctgcatatca tgttttgcta ggtacgcggc aatcagtgaa
    10501 ccgacttccg catcatcttc cacaaatacg atagtgttca taacattcac ggtagatata
    10561 aaaacgtcac aatacaccgc gctgttttta cttactatca atcgtttcta aacaagtttt
    10621 catcaggtat tctactgatg acttgttatg gaagtgttaa ggtaaaaaga tggggctggt
    10681 aatcaaagcc gcgctcggtg cgctggtagt gctgttgatt ggtgttttag caaaaacgaa
    10741 aaattattat atcgccgggc tgattccact tttcccgacc tttgcgctca tcgcgcatta
    10801 tattgttgcc agcgaacgcg gcattgaagc cttacgcgca accatcattt ttagtatgtg
    10861 gtcgattatt ccctattttg tttacctggt gtcgctgtgg tatttcaccg gaatgatgcg
    10921 cttgcccgcc gcttttgttg gttctgttgc gtgttggggg ataagcgcat gggtgttgat
    10981 tatatgctgg ataaagctgc attaacgcag atgacgaccg ccatcaagtg ggaaactgcg
    11041 tccggtgaca aagcaactgg taagtaagta atcgaccagg tcgatcactt ctttctcgcc
    11101 aggcgcggtt ttcatcagtg atttattcag cgcctgttgt cgatattcgg catcatcatg
    11161 ttcattaaac aggatcagcg atggcgcaat agaattcact ttcacttccg gtgccagctt
    11221 gcgggcaaac gagcgggtca tattatccag tgccgctttg cttgcagcat acgcaatatg
    11281 cttgtcgcta ccgcgctcca ccacataatc ggtaaagtga atgatatcgc tggcggcgtg
    11341 tccgtgccca cgcagtaatc tttccagcgc atggttgagc aggtatgggg tattaacgtg
    11401 gatctgcatc atgcaagcca gtacgtcggc cagtggcgca cccggttttt ccgccatcca
    11461 cgcactggcg ttatgcaaaa tagcacgcag accatgggtg ctttttagta cttcatcggc
    11521 aaacgccatc acaccgtcgt tggtcgaaaa atcagcctga atacactgcg cacctgcatt
    11581 aatcagtcca tcaatggctg gatagtgtgt ccgatagctg acaatcaccg gttgcttttg
    11641 attaatgaaa tgccatgcaa gggcgaggcc gatgcgacga cctccgccag taattaatat
    11701 tggcaagggc tgggttttac ccatcgttat ctcctttact atccaacgat gggatgaacg
    11761 ttatcccacc agcatccagg tcgccggaac tgaggcaatt aacagcatgc caatcagcac
    11821 catttcctga cggttcagta cattatcatg tgtatgcgtt ttgcgcgcat acaggaaaac
    11881 cagcagcccc ggtgcataca gaacaacgga taacagcaag tgcattggac cagaggcgta
    11941 caataaccat aagccataaa tgcaggcacc gacacctacc gctttatgca gtggacgggt
    12001 tgcgattttc agcaagaacg cgccgaccag gaaatagggc accagaatca tttctgaggc
    12061 gatggtcagc aacgtgttgt aatcggaacc ggtcagccag atgagtacca gacaaatttg
    12121 cacgcagatg ttggtcaacc acagtgaggc ggatggcgca gcttgtgcgt tctggcgcgc
    12181 aaaaatgcgt gggaatgctt tatgagtggc agccaggaac ggaacttctg ccgccatgat
    12241 ggtccagctt aagtacgcgc cgcaaacgga aacgatcaaa ccggcagcga tgatgatttc
    12301 gccccatggt cccatcattt ccaccatcag accggccatt gatgggttac gaatttcagc
    12361 cagttcagga cgtgccacca cacccagcga aagcagcgtt accagcaagt aaacgcccag
    12421 agcggagaga accgccagta gtgtcgcttt gccaacatca cgtttattac gcgcacgcgc
    12481 agaaacaacc acggcacctt ccacaccaat gaaaacccac agggtgatca gcatggtgtt
    12541 tttcacctgt tcccaaacag gtacgccaag tgcaagtccg gtgaagtcga gcttgaaggt
    12601 atccagtttg aacatcatca tcgccagcac aacaaacaga cccagcggca acaattttgc
    12661 cagtgtcgcc accaggttaa tgctggcagc ggtttgcacc ccgcgcagga tcaaaaagtg
    12721 aacaatccac aataaagccg atgcaccgac tatcgattgc caggtattac catcgccaaa
    12781 caggcgcaat tccggcgtgt ccgtaaaaaa gcttaacgcg gaaaaaacga tcaccagata
    12841 ggagacgttg gcgatgaccg cgcacagcca gtatccccat gcggaacaaa agccgattag
    12901 ctcgccaaac ccttcgcggg cataggtaaa gataccgccg tcaagttcgg gacgaatgcg
    12961 cgtgaggatc agcatggcaa aggccagcaa taaaatgcca gcgccagtaa taccccagcc
    13021 gatgagcagt gctgccgggc tggcaactgc cgccatattt tgcggcagac tgaaaacacc
    13081 cgcgcccagc attgagctta ataccagcgc ggtgagtgcg ctcagtccca gtttcttttc
    13141 catcgatatc cagtgattaa aacgtgataa gaagaataaa tattagggca aaacgcataa
    13201 aaatggagtt tgccactgaa gcgcgggatt ttacgaagag atgtgtctgc atgcaatggt
    13261 cgcagggaaa tttgtgacta aaaatgcgaa aggcggcatc aagccgcctt ataggagtaa
    13321 ctgaatagtt atttatacag atctgcgctg acggtcaggt tattaccacg ttcctgccac
    13381 tggcgggtga tgtggtaata cttagcacct ttcttcgcgg cacgtttcgc aacctgatag
    13441 gagacttcgg tcatgttgcc gtagttgcca gagaatttga tgctgtcgaa cgggaccatc
    13501 atcgctgcgg tcgctttgtt cagttcttcg actttagtgc catccgggag cgtgacggtg
    13561 taacgcccgc cttttgatga ctgggtttca aagaagcgac cgacttcaga acttggtgat
    13621 gcggtagtcg caacacccgg gatctcaact ttcttcgcgg cttcgccacc ggcagccaga
    13681 gctgcacgtc ctgcttcgga atctgccggg atcacatccg ggctctggac gatacgtttc
    13741 ttagcatctt ttttatagat gaatgcagta atacgctggt tgccgccctg gttggcatcg
    13801 atttgacgaa cgatgtagaa agagtaggca cctttcgctt ttgccgcttt ggtgatggca
    13861 tcattgactt ctggctggct acgatagaag ccctggacgg tgaccgtgtc aaacggttca
    13921 atcagaacag cctgatcttt cggcagttcg acaacaccgt taattacgcg attacttgtt
    13981 tcttctgctt tttcagcatc ggctttatag aggtcagcga ccacacgcca gttaccgctg
    14041 ttaccaaaat cagaagtgtc gacaacataa aaagaggcgg caccttcttt atctgcgcga
    14101 cgagaaacgg ctttcaccgc ttcgccaata gcattaaaac gaccggtaac cactacacgg
    14161 tcaaaaggtt taaccgctgc cgcttgctcc ggtgtcagtt ctgttgctgc gttaacggag
    14221 aatgccgtag cagaaagcag tgccgacgcc aggagggtgt tcttaagctt cataaaaata
    14281 atccttcgcc ttgcgcaaac caggtactgg tattgttatt aacgagaaac gtggctgatt
    14341 attgcattta aacggtgtaa ctgtctgcgt catttttcat atcacattcc ttaagccatt
    14401 tttaatcctg ctcaaatgac cgtctatgct taaaaaacag ccgtatcagc atcattacta
    14461 ctgaagcaac tgaattgtat aagttaattt aatgttaagt agtgattcgt gccggggcga
    14521 tgtctcgttt tacccgaccg tcgaagacaa ttatcagtct ttatccggcg ttctaaggtg
    14581 tttatcccac tatcacggct gaatcgttaa tattttgcga gttcacgccg aaatactgat
    14641 ttttggcgct agatcacagg cataattttc agtacgttat agggcgtttg ttactaattt
    14701 attttaacgg agtaacattt agctcgtaca tgagcggctt gtgtggctcc tgacacaggc
    14761 aaaccatcat caataaaacc gatggaaggg aatatcatgc gaattggcat accaagagaa
    14821 cggttaacca atgaaacccg tgttgcagca acgccaaaaa cagtggaaca gctgctgaaa
    14881 ctgggtttta ccgtcgcggt agagagcggc gcgggtcaac tggcaagttt tgacgataaa
    14941 gcgtttgtgc aagcgggcgc tgaaattgta gaagggaata gcgtctggca gtcagagatc
    15001 attctgaagg tcaatgcgcc gttagatgat gaaattgcgt tactgaatcc tgggacaacg
    15061 ctggtgagtt ttatctggcc tgcgcagaat ccggaattaa tgcaaaaact tgcggaacgt
    15121 aacgtgaccg tgatggcgat ggactctgta ccgcgtatct cacgcgcaca atcgctggac
    15181 gcattaagct cgatggcgaa catcgccggt tatcgcgcca ttgttgaagc ggcacatgaa
    15241 tttgggcgct tctttaccgg gcaaatcact gcggccggga aagtgccacc ggcaaaagtg
    15301 atggtgattg gtgcgggtgt tgcaggtctg gccgccattg gcgcagcaaa cagtctcggc
    15361 gcgattgtgc gtgcattcga cacccgcccg gaagtgaaag aacaagttca aagtatgggc
    15421 gcggaattcc tcgagctgga ttttaaagag gaagcgggta gcggcgatgg ctatgccaaa
    15481 gtgatgtcgg acgcgttcat caaagcggaa atggaactct ttgccgccca ggcaaaagag
    15541 gtcgatatca ttgtcaccac cgcgcttatt ccaggcaaac cagcgccgaa gctaattacc
    15601 cgtgaaatgg ttgactccat gaaggcgggc agtgtgattg tcgacctggc agcccaaaac
    15661 ggcggcaact gtgaatacac cgtgccgggt gaaatcttca ctacggaaaa tggtgtcaaa
    15721 gtgattggct ataccgatct tccgggccgt ctgccgacgc aatcctcaca gctttacggt
    15781 actaacctcg ttaatctgct gaaactgttg tgcaaagaga aagacggcaa tatcactgtt
    15841 gattttgatg atgtggtgat tcgcggcgtg accgtgatcc gtgcgggcga aattacctgg
    15901 ccggcaccgc cgattcaggt atcagctcag ccgcaggcgg cacaaaaagc ggcaccggaa
    15961 gtgaaaactg aggaaaaatg tgcctgttca ccgtggcgta aatacgcgtt gatggcgctg
    16021 gcaatcatcc ttttcggctg gctggcaagc gttgcgccga aagagtttct tgggcacttc
    16081 accgttttcg cgctggcctg cgttgtcggt tattacgtgg tgtggaatgt atcgcacgcg
    16141 ctgcatacac cgctgatgtc ggtcaccaac gcgatttcag ggattattgt tgtcggagca
    16201 ctgttgcaga ttggccaggg cggctgggtt agcttcctta gttttatcgc ggtgcttata
    16261 gccagcatta atattttcgg tggcttcacc gtgactcagc gcatgctgaa aatgttccgc
    16321 aaaaattaag gggtaacata tgtctggagg attagttaca gctgcataca ttgttgccgc
    16381 gatcctgttt atcttcagtc tggccgggct ttcgaaacat gaaacgtctc gccagggtaa
    16441 caacttcggt atcgccggga tggcgattgc gctaatcgcc accatttttg gaccggatac
    16501 gggtaacgtt ggctggatct tgctggcgat ggtcattggt ggggcaattg gtatccgtct
    16561 ggcgaagaaa gttgaaatga ccgaaatgcc agaactggtg gcgatcctgc atagcttcgt
    16621 gggtctggcg gcagtgctgg ttggctttaa cagctatctg catcatgacg cgggaatggc
    16681 accgattctg gtcaatattc acctgacgga agtgttcctc ggtatcttca tcggggcggt
    16741 aacgttcacg ggttcggtgg tggcgttcgg caaactgtgt ggcaagattt cgtctaaacc
    16801 gttgatgctg ccaaaccgtc acaaaatgaa cctggcggct ctggtcgttt ccttcctgct
    16861 gctgattgta tttgttcgca cggacagcgt cggcctgcaa gtgctggcat tgctgataat
    16921 gaccgccatt gcgctggtat tcggctggca tttagtcgcc tccatcggtg gtgcagatat
    16981 gccagtggtg gtgtcgatgc tgaactcgta ctccggctgg gcggctgcgg ctgcgggctt
    17041 tatgctcagc aacgacctgc tgattgtgac cggtgcgctg gtcggttctt cgggggctat
    17101 cctttcttac attatgtgta aggcgatgaa ccgttccttt atcagcgtta ttgcgggtgg
    17161 tttcggcacc gacggctctt ctactggcga tgatcaggaa gtgggtgagc accgcgaaat
    17221 caccgcagaa gagacagcgg aactgctgaa aaactcccat tcagtgatca ttactccggg
    17281 gtacggcatg gcagtcgcgc aggcgcaata tcctgtcgct gaaattaccg agaaactgcg
    17341 cgctcgtggt atcaacgtgc gtttcggtat ccacccggtt gcggggcgtt tgcctggaca
    17401 tatgaacgta ttgctggctg aagcaaaagt accgtatgac atcgtgctgg aaatggacga
    17461 gatcaacgat gactttgctg ataccgatac cgtactggtg attggtgcta acgatacggt
    17521 taacccggcg gcgcaggatg atccgaagag tccgattgct ggtatgcctg tgctggaagt
    17581 gtggaaagcg cagaacgtga ttgtctttaa acgttcgatg aacactggct atgctggtgt
    17641 gcaaaacccg ctgttcttca aggaaaacac ccacatgctg tttggtgacg ccaaagccag
    17701 cgtggatgca atcctgaaag ctctgtaacc ctgacggcct ctgcggaggc cgtcactctt
    17761 tattgagatc gcttaacaga acggcgatgc tttgacctcc cgctgtttgt tcaagcgcaa
    17821 ttttgacaat aattgttaac ggcacggaaa gcagcatacc caccggtcct aacaaccatc
    17881 cccaaaaaat caacgacaaa aataccacca atgtggaaag tcccagccca cgccccatga
    17941 tgcgtggctc cagaatattg ccgaagacca gattaatcag cagatatccc gccagcacca
    18001 acaacgcttc gtagaagcca ttaaacacca gtacctgagc gatagggggg attgccgcga
    18061 gaactgaacc aatattaggg atgtaattaa gcgcaaaggc cagcaatccc cagacaaaag
    18121 cgaagcgaac atcgagggcg gcgagcatcg cccatgcgac caggccggtg atgatgctga
    18181 tggctgtttt cagcaccaga taatgagaca cactgtcaat cgcacgctga atcgccgcca
    18241 tcccttcaac cggacgcgcc atcatttgct gaaattttcc gggcaattgt ggcacttcga
    18301 gcagcataaa cagcaccgtc agcagcaata aaaatattga tgacatggca ttagataact
    18361 gcgtcaataa gttggtgagc aacgtcatcg ccgcgttcgg atcaatataa tgcgccagct
    18421 ggtcaactga gacgtcaatc cctacgcgtt gcaacaacgg ttcaagcgct tgcagcggcg
    18481 tcataataga gttgcgatat tgcggtaacg tccgcgtcaa ctcgttgagc gcggaaccca
    18541 agtaagctaa tagcaacacc atcgccatca cgatgatggt catcaaaatc gacactgcca
    18601 gtacacgcgg cacacgccag cggaccatgt gttgcaccag cgggttaaga ataacagcaa
    18661 taaataatgc gagaataaac ggcacgatga tctcggcggc aaaacggata ccgcagagaa
    18721 taatgaccag cattcccaac ataatgacga tttttaggcc attgagcgtg atgatcggct
    18781 ttgccatgct tgtccccgtt ttttcttcct ttgctaataa taatgtcttt ttcgctccgt
    18841 catcataacg aaaatcaaac cgctcaggta aagaaatgaa aatattttga gttaattctt
    18901 aagctatgat acaaatcagg cgtgttcaac taccgaggac aattatcatc cgcgatgacg
    18961 agaagcaaca ctgcggataa ttgtaatatt atggacaatt tgttcaggac gttattttct
    19021 acatttaccc atcttcgtac ctcatccact atcttgctcg taggtgagca gcactggcgc
    19081 aacgcgcttt agtcctttct tctgccaaag cttgcgctaa gcgcggggct tgaccacttt
    19141 actttggttt cgctgaatta agcgaaaatt aaaataattc tcttgcagga gaaggacaat
    19201 gtatatttat tggattttat taggtctggc tattgctaca gaaattaccg gtacgctgtc
    19261 catgaaatgg gcgagcgtca gtgagggaaa tggcggcttt attttaatgc tggtgatgat
    19321 ttctctgtcg tatatatttc tctctttcgc cgttaaaaaa atcgccttag gcgtagctta
    19381 tgcgctgtgg gaaggtatcg gtattttatt tattaccttg tttagcgttt tgttattcga
    19441 cgaaagttta tcgctgatga aaattgccgg gttaaccacc ctggtcgccg ggattgtgtt
    19501 gataaaatca ggtacccgta aagcgcgtaa acctgaactg gaggtgaacc atggcgcagt
    19561 ttgaatgggt tcacgccgcc tggctggcat tggcaatcgt gctggaaatc gttgctaacg
    19621 tctttttgaa attttctgac ggctttcgtc gcaaaatatt tggcttgctc tcccttgcgg
    19681 cggtgctggc tgcctttagt gcgctttctc aggccgttaa agggatcgac ttgtctgtcg
    19741 cttatgcatt gtggggcggg tttggtattg ccgccacgtt agccgcaggt tggatcttgt
    19801 ttggtcaacg gttaaatcgt aaaggctgga ttggcctggt cttgctgttg gctggaatga
    19861 tcatggtgaa acttgcctga tgaagacgct gcccgcgctg tcgggcagcg tttgaacatt
    19921 atttttgcga cagttgatcc agcttgtcgc gaaaaccggt aacagaaatg gcccggttat
    19981 cggcgcgcca gcgatctttc gcggcaggag ccgaactttg caccccaatt aattgccagc
    20041 cgtcatcggt atgcaacatc agaggcgaac cgctgtcacc cggcaaggta tcgcactgat
    20101 gtgacatcac cgacgtttgc gcccagccag tcacttcaca gttttgatga ctgtacaacg
    20161 tatcgagatg atcttcaggg tatcctgcct gagtcacttt acgacctgcc gcttttaatg
    20221 cggcggtaag cgcggcttta tctccctcaa ataacggcaa cggcgtaatg ccagaagggg
    20281 gattacgtag cacaatcaat ccgaagtccc acggcgcggc tgcgggaggt acaatccaac
    20341 catccccatc tgcttttaac cgctttccca gtgtcggatc aacgcggcct tctatgtcgt
    20401 ggatctcata gcgccaaaga cctttatttg acacaaaacg cagcgccacc gctttatcgg
    20461 ctttaccctt tggaggtgtc aataaacagt gtcctgccgt taatgccaga ttgggtgcaa
    20521 tcagcgtcgc cgtacataaa ttgccgctgg ccgtttccag ttgcccaacc gcatcccacg
    20581 gtgattgggt cgtgtcattc actggcacac gatcatcatg accaaaaaac aaggtgctga
    20641 cctcatcgtt tgccgatttg gcaacgtctg gtttatctgc aaacacaaaa gcagacgtca
    20701 aactaattgc acccaacact acagcaatgg ttgtacgcat atcacactct ggtgggtaat
    20761 tatgattatt aaaagcaaac cctcataaat actatagacg ggacgggatg aaagtgggag
    20821 taaaatcaga taactacagt caggaaagat aaaaacgggc ggcaatcagt gcgcataaaa
    20881 taaggatgat aagaatgaac tcaaagcgat aacggcccac catactaccc tccgctaaag
    20941 gcggtgctaa acgttacccg cttagcaccg taattttcag gcgcgagggg gcgcgccagc
    21001 attactgttg aaaacttacg ctgcgggttg tgcagcaggt tttgccggtt gctgatggct
    21061 gtgtttcttg gcgtgtttct tcgctgcctg cgctttttgt tcaggggctt tctgttcagc
    21121 tttcgcattt ttatgatgct ttttagccgc ctgcgctttc tgggcaggtg ctgctttatg
    21181 ctgtttttta tgatgtgtag ttttcgccgg cgctgctttg gtggtcgtcg cagtcggagc
    21241 aggtgtggtc gcagtctctg cagcaaaggc ggcagaagac agacccatag cagcggcaac
    21301 aaccagagct aatacttttt tcattgtcat accctcaatt tgttttttca tttaacccca
    21361 ctgcggggcc gttgaaataa ctatatccct gggaatggca gacttccgtg agtggttggt
    21421 ttcagcgtgt aacgatatgt acaaacgctg aataaattac agcgttgata ataacgtatt
    21481 gtgacttaag ggaaatttag ctaccaataa tagtagtctt gatcgggtta actattttac
    21541 tacttacaaa gcggtagaat aactgcgcat cagtaataaa tgacacacag caaaatgaat
    21601 ccgtttattt gggtacttat aatcctgatg acgctagacg cgctgcggga attggctggc
    21661 gcttcgtcta ttttaggatg gctattaacg ttggtttgag caggcaataa gtccggacgg
    21721 gtatttaccg cagtccggac ttatttttca ggcgtgcaga cgacgatgca aacgcgtccc
    21781 gaccagcaga gcaatgacca gcatcagagc aataaatgcg ccgacgccgt tccagccata
    21841 gttatgccag aaaacaccac ccagcgtccc ggcaatactc gaccccagat agtaactgaa
    21901 cagatacagc gaggaggcct ggcctttagc gcgttttgcg cgggggccga tccagctgct
    21961 ggctactgag tgggctgcga agaatcctgc tgagaagagt aacattccgg caaagatcag
    22021 ccacagcgag ctgaataagg tcatcagtaa accaaacagc ataacccccg tcgaaaacaa
    22081 catcaccgga ccgcgcccat agcgggtggt catggttccg gctttgggtg agctccatgt
    22141 accggtcaaa taagccagcg ataataagcc aaccacggcc tgactgacat gccaggggga
    22201 gagcatcaac cgatagccga tgtaattaaa cagcgtgacg aacgacccca tcagcaaaaa
    22261 gccttctgcg aacaataacg gtaatccccg gtcacgccag tgcaaacgaa agttgataaa
    22321 caacgactta ggtcgcagcg aggtcgggcg aaaatggcgt gattccggga ggattttcca
    22381 gaacatcaac gccgaggcta gcgcgaaaca accgatagcc gccagagcaa ttcgccagtt
    22441 gaaaaagtcc gtgaagacac cactaattaa gcgtccgctc atgccgccaa ttgagttgcc
    22501 gctgatatac aaccccatcg aaaaggccac gaaactgggg tggatttcct cgctaagata
    22561 agtcatgcca acagctgcca cgccacttaa cgaaagccca atcaaggcgc gcataatcaa
    22621 aatgccgtgc cagctggtca tcattgtcga aagtaacgta caaatggagg ccaacagtag
    22681 cgccgtaacc atcactggtt tgcgaccaat ggcatcggat agggggccag taaacagcaa
    22741 accaatagcc aacatcgccg tggaaatgga cagtgaaata ctactgttcg cgggcgttaa
    22801 gccaaactcc tgcgaaagca ccggaaggat aggctgcaca caatagagaa gtgcaaatgt
    22861 tgccagtccg gcagagaaca gcgccagggt gacgcgcata aattgcggcg taccgcgttt
    22921 aataaattga tttggctgag aaatgctttg cttgtcagtg tcgcttgccg gagcgccatc
    22981 aacagttgta gtacggctca cttgaaatcc ttgctaaata tgcctgtgga tcaggcttat
    23041 acataggtta ggaaaatcga attgttctgt ctaatatatt aataatctca aataagatgt
    23101 tttaaatatg aatattgaac ttcgtcatct gcgttacttt gttgctgttg cggaagagct
    23161 gcatttcggg cgcgccgctg cccgcctgaa tatttcgcaa ccgccgctaa gtcagcagat
    23221 tcaggcgctg gagcaacaaa ttggtgcccg actgctggca cgaaccaatc gcagtgtatt
    23281 gctgacggca gcaggaaaac agtttcttgc agatagtcgg caaatcctgt ctatggtgga
    23341 tgacgctgcc gctcgcgctg aaagactgca tcagggtgaa gcgggggagt tgcgcattgg
    23401 ttttacttcg tcggctcctt ttattcgggc ggtgtccgat acgttatcgc tgtttcgccg
    23461 agattatccc gatgtccatt tacaaacccg cgaaatgaac actcgcgagc aaatcgctcc
    23521 gctcattgaa ggaacgctgg atatgggatt gctgcgtaac acagcgttac cggagacgct
    23581 tgaacacgca gtcatcgtcc atgaaccgct tatggcgatg atcccgcacg atcatcccct
    23641 ggcaaataac ccgaatgtaa cgctggctga actggcgaaa gaaccctttg tcttttttga
    23701 tccgcacgtc gggacagggc tgtatgacga tattctcggg ctgatgcgac gttaccattt
    23761 gacgcccgtc atcactcagg aggtgggcga ggcaatgacc atcatcggtc tggtttccgc
    23821 cggtctgggt gtttcaattt tgcctgcgtc atttaaacgt gttcagctca acgaaatgcg
    23881 ctgggtgccg attgctgaag aggatgcggt ttctgaaatg tggttggtct ggccgaaaca
    23941 tcatgaacaa agtccagctg cgcgtaactt tcgtattcat ctgctgaatg ctctcaggtg
    24001 agggaaattt cagcgaaaaa gcccgaaaaa tgtgctgtta atcacatgcc taagtaaaaa
    24061 tttgacgaca cgtattgaag tgcttcacca tagcctacag attatttcgg agcgcgaaaa
    24121 tatagggagt atgcggtggt tgctgaaaac cagcctgggc acattgatca aataaagcag
    24181 accaacgcgg gcgcggttta tcgcctgatt gatcagcttg gtccagtctc gcgtatcgat
    24241 ctttcccgtc tggcgcagct ggctcctgcc agtatcacta aaattgtccg tgagatgctc
    24301 gaagcacacc tggtgcaaga gctggaaatc aaagaagcgg ggaaccgtgg ccgtccggcg
    24361 gtggggctgg tggttgaaac tgaagcctgg cactatcttt ctctgcgcat tagtcgcggg
    24421 gagattttcc ttgctctgcg cgatctgagc agcaaactgg tggtggaaga gtcgcaggaa
    24481 ctggcgttaa aagatgactc gccattgctg gatcgtatca tttcccatat cgatcagttt
    24541 tttatccgcc accagaaaaa acttgagcgt ctaacttcga ttgccataac cctgccggga
    24601 attattgata cggaaaatgg cattgtacat cgcatgccgt tctacgagga tgtaaaagag
    24661 atgccgctcg gagaggctct ggagcagcat accggcgttc cggtttatat tcagcatgat
    24721 atcagcgcat ggacgatggc agaggccttg tttggtgcct cacgcggggc gcgcgatgtg
    24781 attcaggtgg ttatcgatca caacgtgggg gcgggcgtca ttaccgatgg tcatctgcta
    24841 cacgcaggca gcagtagcct cgtggaaata ggtcacacac aggtagaccc atatggtaaa
    24901 cgctgttatt gcgggaacca cggctgcctc gaaaccatcg ccagtgtgga cagtattctt
    24961 gagctggcac agctgcgtct caatcaatcc atgagctcga tgttacatgg gcaaccgtta
    25021 accgtggact cattgtgtca ggcggcattg cgcggcgatc tgctggcaaa agacatcatt
    25081 accggggtgg gcgcgcatgt agggcgcatt cttgccatca tggtgaattt atttaaccca
    25141 caaaaaatac tgattggctc accgttaagt aaagcggcag atatcctctt cccggtcatc
    25201 tcagatagca tccgtcagca ggcccttcct gcgtatagtc agcacattag cgttgagagt
    25261 actcagtttt ctaaccaggg cacgatggca ggggctgcgc tggtaaaaga cgcggtgtat
    25321 aacggttctt tgttgattcg tctgttgcag ggttaacatt ttttaactgt tctaccaaaa
    25381 tttgcgctat ctcaatttgg gccaggaaag cataacttag actttcaagg ttaattattt
    25441 tcctggttta tatttgtgaa gcataacggt ggagttagtg atgctgaagc gtttctttat
    25501 taccggtaca gacacttctg tagggaaaac ggtggtttcc cgcgcattgc tacaagcgtt
    25561 agcctcccag ggaaaaacgg ttgcgggata taaacccgta gcgaagggga gcaaagagac
    25621 acccgaaggg ctgcgtaata aagatgccct ggtgttgcag agtgtttcaa ccatcgaact
    25681 gccttatgaa gcagttaatc ctatcgcgtt aagcgaagaa gaaagtagcg tagcgcacag
    25741 ttgcccaatc aattacaccc tcatttcaaa cggcctggca aacctgaccg ataaagtcga
    25801 tcatgtcgtg gtagaaggga ctggcggctg gcgcagtctg atgaatgatt tgcgtccact
    25861 ctctgaatgg gtggtgcagg aacaactgcc ggtgttgatg gttgtcggta ttcaggaagg
    25921 ttgcattaac catgcactgc taacagctca ggcgattgcc aacgacgggc tgccgctcat
    25981 tggctgggtg gctaaccgaa tcaacccagg actggcgcat tatgcggaaa tcattgatgt
    26041 gctcggtaaa aaacttccgg caccgctcat tggtgaactg ccttatctgc cgcgcgccga
    26101 acagcgtgaa ctggggcaat acatccgctt agctatgctg cgcagtgtgc tggcggtaga
    26161 tagagtcacg gtgtaacgtc cgcgagatta ccgacgcaat tacgcaggca atcaataaac
    26221 cggggagtag ctgatactcc ccggtcattt cacatatcat caacgtcgac ataatcggcg
    26281 cgtgcgtggt tgccgccaac agtgtcgcca tcccggtcaa tccgagtaaa agcgtaattt
    26341 cttcgccatc agggaaccat aatcccaggc tacgaccata caacatgcca atggcaagac
    26401 cgataaatag cgtcggtgta aagacaccac cgggtgcgcc ggaaccgcta ctcgccagca
    26461 cggcacacag tttacaaagg aagatcccgg caatgatcat taacagtggc ggggcggtta
    26521 aaaaggattg tacggtgcta tagccgttgc cccacactgc aggtgtgaac agggaaagca
    26581 gacccacgat caacccgcct agtgccagtt gccagggcgg cgcaagtttg agactcacaa
    26641 atccacgatg acaggcgttc attaacgtta acaacagtgg tccgcacaga cctgccagca
    26701 cacctgtact gataatcagc gcatagtcac gagcctgaac cgtcactgag agttgtacgc
    26761 tgtagagtaa cgcgtcgcta tgattaatca gattgctaac cagcaatgcc acgacggcgg
    26821 aaataatcac cgggccgaga gaggccaaca tcatagtgcc aaacagcact tcggctataa
    26881 ataaactgcc agcaagcggg gcacgatagg ccgcagccat tcccgccgcg gccccacagg
    26941 cgatccataa tttccactcc tggcgtggcg taaaacgttg ggcaaaacag gaggcggcaa
    27001 gggcagctaa aagaatcatc gcaccttcgc gaccaattgc actgccgctg gttactacca
    27061 gcagagaggc aagcgattta accaggcttg ctgcgtaatc aaactgacca tcggtttgca
    27121 acgcttccat ataatcggtc ggcgcatgag ggcgttgctg ggtaaatttc tgccagccca
    27181 tcagcaacaa acccgccgcc agtccgccga gcgccggagt tagcagccgt cgccaggggg
    27241 aaaggtttgt cgctgcattg accagactgc cggagtcatt attgaggaac aaccactcca
    27301 gtagcagcat cgcatgacga aacccggcaa cggcaaaggc cgcgagaata ccgacgactg
    27361 ttgcgataag cagacggcgg aacatagtgc gcagattcgg atagatgtgt agatgatgca
    27421 tgagcagaaa tgggcaatga aacacgggcg actattttgc ggcaaaagag gccagccgca
    27481 aaagcagtgc gtgctttaag agataaaaaa aaacgtgaca ctgtaacgta ttattgccga
    27541 attgcgcgta atcgtcacca tccggcaata ttatggtgat cctatcgaaa cagcggttta
    27601 accgcgacag gaattaacag ttgtgattgc cattgcgcca gcgttaagcg agccagttta
    27661 cccagtgcac ggtagaaagg gtgttctgct ttttcgataa aaacgtcaag aaaacgtgtt
    27721 gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt ctgtctggcg accattctcc
    27781 gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat cttccggttc gttttgcttc
    27841 atttcaaact gaatgccttt ctcgcgcatc cactgacgaa gtgccaatgt tgaatcgcca
    27901 aacagcacag attcgcgatc cagccagacc gacccccacg gcggtgaagg cagtgcccac
    27961 gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct cttcacactg tgtctgaaaa
    28021 gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc actgagtttc ccagccgtca
    28081 cgggtgagta ccgcaaccag aggtgcggct tccgcactct ctggagcgta ataaaacagc
    28141 gcgcccaaca cgcgcgccac gacagaaaaa ttatcttgct gtgaaaaatg ggtcattcct
    28201 aacatcctga aatgtgcggg ttgccccgca ccaatttgtt aacctgcaat agccattcct
    28261 accgtcatgt gcaggccata aaagagcaca cgcccgatca tctcaccgcc aagaatgagg
    28321 attaacccca gaataagtcc ggcaacgtga ggttcacggc gacgaatgag cggacaaagc
    28381 cagcatccta gacctgcgca taacaatacc acacgccaga cctgtaagga ggcgtaatct
    28441 ggtaccagtg cgctggcttg ttgcacggat gagtgaatag acgacagaga caaaccttgc
    28501 agcacaatca ccccggcaca agcgatcagc gccagaacgc taatgatggc aaatggtgtg
    28561 gtattaaatg taacgcgggc tgcccgcaga attgcggcag caagtattgg accactcaac
    28621 aataccgtca ggaagaaagc cagcgtagtg taaccgttat gccaggttgg cacagtgtcg
    28681 atttgataca cgcaggtcat catccagacg aaaatgacgc cgagcgccat gctgacaagt
    28741 aaccagagtt tccccaacgc ttgcggcatt ttaccgatga ccgccaccag ccaccatagt
    28801 ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt tacttagacc agaagctccg
    28861 attcgattca gcgagttaaa cgcccgcagt ggcgatccca ggtgcatgac agaggcaata
    28921 aacccgatgc ccatcaacag ccagaggaaa aacatgccac gaacaatgtg ctgacggtct
    28981 gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa tcagcgcgcc tactacgcat
    29041 tgacccaata cggtaaagat aaccagtggc cattcatgcc atccatttcc cattttacac
    29101 ttcctccgga ttagccagat aaccagtggt atcgccggtc gggcggctgt tggcgttagg
    29161 tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc agtggcgcga cggcagccag
    29221 agtgccgtgc ttctggcgca gctcttcaat tggaccgaac tcaagtgcac gcagcgggca
    29281 ggattcgaca catatgggtt gtttcccctc ggcgacgcgc gaataacaac catcgcactt
    29341 cgtcatgtgc cctttttcag cattgtactg tggcgcgccg tacggacaag ccatgtggca
    29401 gtagcggcag ccgatacaaa catcttcatc aaccaccaca aagccatctt cacgcttatg
    29461 cattgcgccg ctagggcaga cttttgtaca tgcggggtca tcgcaatggt tacaggagat
    29521 ggagagataa taagcgaaca cgttctggtg ccagacgcca ttatcttcct gccagtcgcc
    29581 gccagcatat tcataaatac ggcggaaact gacttccggg ccaagatctt taaaatcttt
    29641 gcacgccagt tcgcaggttt tacagccagt acagcggctg gaatcgataa aaaatccata
    29701 ttgtgtggtc atgggctact ccttaaacct tttcgatctg gacaagattg ctgtgcgacg
    29761 ggtttccctt tgccagcggt gaagggcggt gagaggtcag aatattaata ctgccgccgt
    29821 gatcgacccg gtcaccaaac atatccgctt taagccacgc accttgcccg atggcggtaa
    29881 cgccaggcag aatacgcgga gtcacttttg cggcaatcag catttctcca ttattgttaa
    29941 atacccgcac ggtatcgcca tgacggatac cgcgagcctg agcatcaatg gggttgatcc
    30001 acacctcttg tgggcaggcc tgctgtaaca catcaatatt gccgtagctg gagtgggtac
    30061 gcgctttgta gtggaagccc gttaactgca gtggataggt tttccgcagg ggatcgtccc
    30121 agccatcaaa acctggggta tacgcaggaa ggggatgaat aatttcatct tttttcaatt
    30181 cccaggtatc tgcaatcttc gccagtcgtt cagaataaat ttcgattttc cccgaaggtg
    30241 ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg gaaagcgacg tagtgttctt
    30301 ccgggcattt tttcttaaag atcccggtcg ttttcatctc ctcgtagtcg ggcatcttag
    30361 ggttacgttc cttcgttttc gcatggagat atttgatcca ttcatgctga ctgcgacctt
    30421 cagtaaaggt ttgataaacg tccggtccta agcgtttggc gacttcactc agcatccagt
    30481 agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc acatagccca
    30541 tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc aggtcgggca
    30601 acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca acaatcatct
    30661 cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca tgttgattga
    30721 tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt ttttcttttc
    30781 cacgaacacc atcacgggtc gcggtcattt ccgtaccatg gtcgatggca tctgtccatg
    30841 taaagacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat tctaccccca
    30901 gatcccagct accttcgcgt acgcctgagt tgccgccgtt tatgccgacg ttaccggtga
    30961 gaacggaaag catggcaata gcgcgggatg tttgttcgcc gttggagtgt cgttgtggcc
    31021 cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc aactggataa
    31081 ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta gctatgccgt
    31141 caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg ttggcgggca
    31201 gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg accatgtttt
    31261 cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca gggcgaatgg
    31321 gcagccattc atcttcacgc ccggcagcag tgtcgttata acgtggatcg atgacgatca
    31381 tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca ccgccgctca
    31441 tccgcgtttc tgctgggtta tttccgaaca taacgaccag tttcgtattg gcgatatcat
    31501 ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg atctgtgcgg
    31561 tactgtagct accatagcga ctgagaaagc caccgcaaga gttcatcaga cggtacggga
    31621 cgtttgagtt ggtgatgttt ccgccatcta cacctgttcc gtacaggaca tgtacagcct
    31681 cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc agggcttcgt
    31741 cccaacttat ccgttcaaat ttaccttcac cgcgcttgcc gacgcgcttc atgggatatt
    31801 tcaacctatc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa cacgctcgaa
    31861 cctgatgatt accgtagacg tcgtcacctg tcgtatcaga ctccacccag tacacggtgt
    31921 catctttcac atgcaaacgt aacagacagc ggctcccgca gttaacggtg caggaactcc
    31981 agaccgcttt ctcttctacc ggagcctctg ccgcccggac catttgggaa aatggcagag
    32041 tgaaagcact gcttgccagc gcaagactgc caagtgcgga ggttttcatc agacttctac
    32101 ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt actcacttat
    32161 tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat attttttcga
    32221 tctcgaccag attcgtatgc tgcgggtttc ccttcgccag tggtgaaggg cgcagagtgg
    32281 ttagcgtatt cacacagcct ccatggtcga ttttatcgcc agacatattg gcctcgtgcc
    32341 aggctccctg gcccatagcg ctaaccccag ggagaatgcg tggtgtcact ttggctggta
    32401 gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca atcccacgtt
    32461 tctgcgcatc tatagggttg atccacacct cctgacggca ggcagccttc aggagatcaa
    32521 tattgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt tgcagtggga
    32581 aggttctacg ttcaggtgag tcccagcctt caaaggtaga ggcataaact ggcagtgggc
    32641 ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc ttgctggaat
    32701 aaatttcaat cttaccggaa ggtgttttaa gtggatttgc ctcggggtcg tcacgaaatg
    32761 ctttgtaggc gacaaaatga ccattgggat ctttacgctt atagataccc atttttttca
    32821 gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac agatgttgta
    32881 accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt ccaagacgtt
    32941 tcgcgacttc actcaggatc cagtaaatcg gtttgcgttc gaatttttcg ctggtgacag
    33001 gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata atatcttcct
    33061 gctcaacggt catcaggtct ggcagcagaa tgtcggcata ttttgccgat gaggtcataa
    33121 agttttcgat gaccacaatc atttcgcatt tcgattcgtc ttgcagaatt tcatgcgttt
    33181 tgttgatgtc agaatgctga ttaaccaggg tatttcccgc gtagttccag atgaacttaa
    33241 tgggcacatc cagtttatct ttgccgcgga cgccgtcgcg gattgccgtc atttgcgggc
    33301 catgatcgat agcatccgtc cagctgaagc aggagattga cgttttgacc ggattatcca
    33361 gcaccggcag gcgctctatg gtaatggtat aggtcgattc acgcgcgcca ctatttccgc
    33421 cgctgatgcc gacattgccc gtcaaaatag gcaacatagc aatagcgcgt gcagtcagct
    33481 cgccgtttgc ctggcgttgc ggcccccagc cctggcagat ataagcgggt tttgctgtgc
    33541 caatttcacg cgccagtttg atgatacggt ccaccgggat accggtaatt tgcgaagccc
    33601 actgcggcgt tttcgctgtg ttatcatcac cttcaccaag aatataggct ttatagtgac
    33661 cattttttgg tgcatctgcg ggtaaggttt tttcgtcata gccgacgcag tatttatcga
    33721 gaaaaggttg atcaacgaga ttttcgttaa tcaataccca ggcaatcccc gcaaccagcg
    33781 cggcatcggt gcccgggcga ataggaagcc attcatcctc acgaccagca gccgtatcgg
    33841 tatatcgcgg atcgataaca atcattttgg cgttcgattt ctcgcgcgct ttttcaagaa
    33901 gataagtgat gccaccgccg ctcatgcggg tttctgccgg gttgttacca aacatcacga
    33961 ccagcttgct gttttcaata tccgtggtgc tgttgccatc attactgccg taggtgtagg
    34021 gcatggcaca ggaaatttgt gcggtgctgt aggagccata ctgattgagt gaaccgccgt
    34081 agcagttcat caggcgtttg accgccgagg ctgatggcga agagcgggtc atattgccgc
    34141 caacgatccc cgaagagtac tgaatatata cagcctcatt gccatattgt tcgacggttt
    34201 tcttcaggct actggcgata gtatccaggg cttcatccca gctaatccgt tcgaatttgc
    34261 cttcaccgcg tttacccacg cgtttcattg ggtaattcaa gcgatcggga tgattaatac
    34321 gccggcggat ggagcgaccg cgcaaacagg cgcgtacctg atggttgcca tactcatcgc
    34381 tgccggtgtt gtcagtttcc acccaggcca cttcattatc tttaacatgt agacgaagtg
    34441 cacagcggct accacagttg acggaacagg caccccagac cacttgttcg ctggcctgtt
    34501 gtactgctgc cgctgcactg cgcagggtga acggcaaaga aaaaccgcct gcagccagcg
    34561 ccagagaacc tatcgcggta gatttaacga gtgttctgcg gctgatgccc accatttgtt
    34621 catttttgga cataactcac tccctgttct ttatcgttat ataaaagttt atatattgaa
    34681 tat
//
LOCUS       DAZKFU020000017        34352 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZKFU020000017 DAZKFU020000000
VERSION     DAZKFU020000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.017"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(18..1877)
                     /gene="gss"
                     /locus_tag="ACHOQQ_RS03355"
                     /old_locus_tag="pgaptmp_000671"
     CDS             complement(18..1877)
                     /gene="gss"
                     /locus_tag="ACHOQQ_RS03355"
                     /old_locus_tag="pgaptmp_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGU4468969.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2082..2948
                     /gene="yghU"
                     /locus_tag="ACHOQQ_RS03360"
                     /old_locus_tag="pgaptmp_000672"
     CDS             2082..2948
                     /gene="yghU"
                     /locus_tag="ACHOQQ_RS03360"
                     /old_locus_tag="pgaptmp_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGU4468970.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3071..3319)
                     /gene="hybG"
                     /locus_tag="ACHOQQ_RS03365"
                     /old_locus_tag="pgaptmp_000673"
     CDS             complement(3071..3319)
                     /gene="hybG"
                     /locus_tag="ACHOQQ_RS03365"
                     /old_locus_tag="pgaptmp_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGU4468971.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3332..3673)
                     /gene="hypA"
                     /locus_tag="ACHOQQ_RS03370"
                     /old_locus_tag="pgaptmp_000674"
     CDS             complement(3332..3673)
                     /gene="hypA"
                     /locus_tag="ACHOQQ_RS03370"
                     /old_locus_tag="pgaptmp_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4468972.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIIYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3666..4154)
                     /gene="hybE"
                     /locus_tag="ACHOQQ_RS03375"
                     /old_locus_tag="pgaptmp_000675"
     CDS             complement(3666..4154)
                     /gene="hybE"
                     /locus_tag="ACHOQQ_RS03375"
                     /old_locus_tag="pgaptmp_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGU4468973.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPLMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4147..4641)
                     /locus_tag="ACHOQQ_RS03380"
                     /old_locus_tag="pgaptmp_000676"
     CDS             complement(4147..4641)
                     /locus_tag="ACHOQQ_RS03380"
                     /old_locus_tag="pgaptmp_000676"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGU4468974.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4641..6344)
                     /gene="hybC"
                     /locus_tag="ACHOQQ_RS03385"
                     /old_locus_tag="pgaptmp_000677"
     CDS             complement(4641..6344)
                     /gene="hybC"
                     /locus_tag="ACHOQQ_RS03385"
                     /old_locus_tag="pgaptmp_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGU4468975.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6341..7519)
                     /gene="hybB"
                     /locus_tag="ACHOQQ_RS03390"
                     /old_locus_tag="pgaptmp_000678"
     CDS             complement(6341..7519)
                     /gene="hybB"
                     /locus_tag="ACHOQQ_RS03390"
                     /old_locus_tag="pgaptmp_000678"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGU4468976.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7509..8495)
                     /gene="hybA"
                     /locus_tag="ACHOQQ_RS03395"
                     /old_locus_tag="pgaptmp_000679"
     CDS             complement(7509..8495)
                     /gene="hybA"
                     /locus_tag="ACHOQQ_RS03395"
                     /old_locus_tag="pgaptmp_000679"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGU4468977.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8498..9616)
                     /gene="hybO"
                     /locus_tag="ACHOQQ_RS03400"
                     /old_locus_tag="pgaptmp_000680"
     CDS             complement(8498..9616)
                     /gene="hybO"
                     /locus_tag="ACHOQQ_RS03400"
                     /old_locus_tag="pgaptmp_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGU4468978.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVIGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9805..10092)
                     /gene="yghW"
                     /locus_tag="ACHOQQ_RS03405"
                     /old_locus_tag="pgaptmp_000681"
     CDS             complement(9805..10092)
                     /gene="yghW"
                     /locus_tag="ACHOQQ_RS03405"
                     /old_locus_tag="pgaptmp_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGU4468979.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10211..11098)
                     /gene="yghX"
                     /locus_tag="ACHOQQ_RS03410"
                     /old_locus_tag="pgaptmp_000682"
     CDS             complement(10211..11098)
                     /gene="yghX"
                     /locus_tag="ACHOQQ_RS03410"
                     /old_locus_tag="pgaptmp_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGU4468980.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11255..12295
                     /gene="gpr"
                     /locus_tag="ACHOQQ_RS03415"
                     /old_locus_tag="pgaptmp_000683"
     CDS             11255..12295
                     /gene="gpr"
                     /locus_tag="ACHOQQ_RS03415"
                     /old_locus_tag="pgaptmp_000683"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGU4468981.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12335..12829)
                     /locus_tag="ACHOQQ_RS03420"
                     /old_locus_tag="pgaptmp_000684"
     CDS             complement(12335..12829)
                     /locus_tag="ACHOQQ_RS03420"
                     /old_locus_tag="pgaptmp_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGU4468982.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13020..13904
                     /gene="yghA"
                     /locus_tag="ACHOQQ_RS03425"
                     /old_locus_tag="pgaptmp_000685"
     CDS             13020..13904
                     /gene="yghA"
                     /locus_tag="ACHOQQ_RS03425"
                     /old_locus_tag="pgaptmp_000685"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4468983.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIHLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVS
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14176..14601)
                     /gene="exbD"
                     /locus_tag="ACHOQQ_RS03430"
                     /old_locus_tag="pgaptmp_000686"
     CDS             complement(14176..14601)
                     /gene="exbD"
                     /locus_tag="ACHOQQ_RS03430"
                     /old_locus_tag="pgaptmp_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGU4468984.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14608..15342)
                     /gene="exbB"
                     /locus_tag="ACHOQQ_RS03435"
                     /old_locus_tag="pgaptmp_000687"
     CDS             complement(14608..15342)
                     /gene="exbB"
                     /locus_tag="ACHOQQ_RS03435"
                     /old_locus_tag="pgaptmp_000687"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4468985.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15594..16781
                     /gene="metC"
                     /locus_tag="ACHOQQ_RS03440"
                     /old_locus_tag="pgaptmp_000688"
     CDS             15594..16781
                     /gene="metC"
                     /locus_tag="ACHOQQ_RS03440"
                     /old_locus_tag="pgaptmp_000688"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGU4468986.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16921..17580
                     /gene="yghB"
                     /locus_tag="ACHOQQ_RS03445"
                     /old_locus_tag="pgaptmp_000689"
     CDS             16921..17580
                     /gene="yghB"
                     /locus_tag="ACHOQQ_RS03445"
                     /old_locus_tag="pgaptmp_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGU4468987.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17620..18519)
                     /gene="yqhC"
                     /locus_tag="ACHOQQ_RS03450"
                     /old_locus_tag="pgaptmp_000690"
     CDS             complement(17620..18519)
                     /gene="yqhC"
                     /locus_tag="ACHOQQ_RS03450"
                     /old_locus_tag="pgaptmp_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGU4468988.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18713..19876
                     /gene="yqhD"
                     /locus_tag="ACHOQQ_RS03455"
                     /old_locus_tag="pgaptmp_000691"
     CDS             18713..19876
                     /gene="yqhD"
                     /locus_tag="ACHOQQ_RS03455"
                     /old_locus_tag="pgaptmp_000691"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGU4468989.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19981..20808
                     /gene="dkgA"
                     /locus_tag="ACHOQQ_RS03460"
                     /old_locus_tag="pgaptmp_000692"
     CDS             19981..20808
                     /gene="dkgA"
                     /locus_tag="ACHOQQ_RS03460"
                     /old_locus_tag="pgaptmp_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGU4468990.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21008..21934
                     /locus_tag="ACHOQQ_RS03465"
                     /old_locus_tag="pgaptmp_000693"
     CDS             21008..21934
                     /locus_tag="ACHOQQ_RS03465"
                     /old_locus_tag="pgaptmp_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HGU4468991.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSVRIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITETD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRIDEIRVLEKTADSARIHIRFTLTNGNNEEQELVLQR
                     RDGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21983..22240
                     /gene="yqhH"
                     /locus_tag="ACHOQQ_RS03470"
                     /old_locus_tag="pgaptmp_000694"
     CDS             21983..22240
                     /gene="yqhH"
                     /locus_tag="ACHOQQ_RS03470"
                     /old_locus_tag="pgaptmp_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGU4468992.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22283..24502)
                     /locus_tag="ACHOQQ_RS03475"
                     /old_locus_tag="pgaptmp_000695"
     CDS             complement(22283..24502)
                     /locus_tag="ACHOQQ_RS03475"
                     /old_locus_tag="pgaptmp_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGU4468993.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGGRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24613..26025)
                     /gene="ftsP"
                     /locus_tag="ACHOQQ_RS03480"
                     /old_locus_tag="pgaptmp_000696"
     CDS             complement(24613..26025)
                     /gene="ftsP"
                     /locus_tag="ACHOQQ_RS03480"
                     /old_locus_tag="pgaptmp_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGU4468994.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26100..26837)
                     /gene="plsC"
                     /locus_tag="ACHOQQ_RS03485"
                     /old_locus_tag="pgaptmp_000697"
                     /gene_synonym="parF"
     CDS             complement(26100..26837)
                     /gene="plsC"
                     /locus_tag="ACHOQQ_RS03485"
                     /old_locus_tag="pgaptmp_000697"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGU4468995.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27071..29329)
                     /gene="parC"
                     /locus_tag="ACHOQQ_RS03490"
                     /old_locus_tag="pgaptmp_000698"
     CDS             complement(27071..29329)
                     /gene="parC"
                     /locus_tag="ACHOQQ_RS03490"
                     /old_locus_tag="pgaptmp_000698"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGU4468996.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMLRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29467..31074)
                     /locus_tag="ACHOQQ_RS03495"
                     /old_locus_tag="pgaptmp_000699"
     CDS             complement(29467..31074)
                     /locus_tag="ACHOQQ_RS03495"
                     /old_locus_tag="pgaptmp_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4468997.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTILQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQALLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31183..31665)
                     /locus_tag="ACHOQQ_RS03500"
                     /old_locus_tag="pgaptmp_000700"
     CDS             complement(31183..31665)
                     /locus_tag="ACHOQQ_RS03500"
                     /old_locus_tag="pgaptmp_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGU4468998.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(31718..32110)
                     /gene="ygiW"
                     /locus_tag="ACHOQQ_RS03505"
                     /old_locus_tag="pgaptmp_000701"
     CDS             complement(31718..32110)
                     /gene="ygiW"
                     /locus_tag="ACHOQQ_RS03505"
                     /old_locus_tag="pgaptmp_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGU4468999.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32262..32921
                     /gene="qseB"
                     /locus_tag="ACHOQQ_RS03510"
                     /old_locus_tag="pgaptmp_000702"
     CDS             32262..32921
                     /gene="qseB"
                     /locus_tag="ACHOQQ_RS03510"
                     /old_locus_tag="pgaptmp_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGU4469000.1"
                     /translation="MRVLLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALGERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32918..34267
                     /gene="qseC"
                     /locus_tag="ACHOQQ_RS03515"
                     /old_locus_tag="pgaptmp_000703"
     CDS             32918..34267
                     /gene="qseC"
                     /locus_tag="ACHOQQ_RS03515"
                     /old_locus_tag="pgaptmp_000703"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGU4469001.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFVRTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
BASE COUNT         8396 a         9209 c         8694 g         8053 t
ORIGIN      
        1 ggttgctaca acatgtatta ctttttcacc acaattaacg gttcaatatc actctctttt
       61 ttaataacca gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc gccaacggtg
      121 aaggtacata cctgaatgta tttaccgtcc actttcggca aacaccacag ttgctgatag
      181 atgtttttct gctcggcaaa tttaccactg gttttgtcca gcacctcttc atgatggctg
      241 acgaggtcga tgttactgcc acagcgaccg gcgatcggtt tcactgcgta acctgttttc
      301 accaattcat cattaacagt gaaatcggta tccagcaggt aacgatggtg cgggaacagc
      361 gaccagagga tcggcagaat cgctttgttg ccggggatca ccgtccacag cggctcaaag
      421 accagcactt ccgggcgcag caatacgtcg ataagacgca cttcattttg cggatgaccg
      481 gtacggatcg gcaccgcagc aaactcacgg tcgctaactt cacgaatctg atcaaacgcg
      541 gtttcccacg cccaggtttt ccacacgcag ttaaccagtc gcccttcccc atcaatcagt
      601 tgcccggcag catcccagcc cagttcatcc agcccgcgca agatacgcgt ttcaaagccc
      661 gcctggtgca gcgcctgctc cataaactgc gcgtgatagt tttcctcgat atctttgtcc
      721 tgcatgatat ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc cagttcgtta
      781 atcagccctt ccgccggatt gaagccgttg cctttatagc cctgctccgc ccaacgttcg
      841 aggatcaagc ccgcttcggt atgacatgag gcggagtcgg cgttgtactc gtaaaccttc
      901 agaccacgct catccatgca gaaatccata cgaccggtga tcatatggtg acggcgacgc
      961 tgccaggaga gacgcaaacg tggccagagg attttcggaa tgtcgaacag cgccagcagg
     1021 ttgtcatctt tcagcacctt gtcggttgcg tgaagataca tcaggtgcag ctcgttggtg
     1081 gctttaatca gctcctgctc ggcgctctcg gtaatggtgt agtaatgata cggatcctga
     1141 ttgatcacct gaccgttggc ctgcacatag gcgttttgca gcggatcttt ttcatccagc
     1201 catttaccgt caaactggcc tttgttttcc agacgcgctc cgctgatttt cagcagctcg
     1261 cctgcaattt ccggctgcgg taagctgtat tcagtatctt ccgtctggat catccagccc
     1321 agaatggtgg tgtcatcaaa agtgtcttta agggtatagc agccgttttc gaccaccatc
     1381 tccagttcgc gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc
     1441 gcaatacgga ctttgttgcc atgtaattgg gtaatgatgg cgacatggcc agtgtcttta
     1501 aattcaccgc ctttatccca gataagaagc gcacccgcga ccggcgcacg cggcgagccg
     1561 ttaggaaatg cctgcaatgg caggatgttg tcattaacca cttcacgcag gaagcgcagc
     1621 gagaaaatct cccacgccat acccacgtca gtaaagacca caccgtagtt cagaaagaga
     1681 aaacggcgag caaattcaac gcattgccat ttgtggccca tatattcgtc gtcgatatag
     1741 ctacggaata cggcgtcatc ttcgtattcc tgcggatcga gagaactgta atctgaagag
     1801 tagattgcta ccccacctgg ggcgtagccc aataatgtcc cgaacggggc atcctggctg
     1861 gtcgttcctt tgctcatcac tttaccttta acaatacaac ctaagcagtt ggcgtaattg
     1921 ttgtgctctg attacctgct cagcgacatt aaccggacag aggtcagact aatcatacac
     1981 ctcatcgcag cggctggcgc aaagggttaa aaaattcaca ttctgtacag caagtgacct
     2041 gctacactgc ttcaacacta ccactcagaa ggcaactcac tatgacagac aatacttatc
     2101 agcccgcgaa agtctggacg tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc
     2161 gcccggtttc tggtccgacg catgaaaaaa cgctgccggt gggcaaacat ccattacaac
     2221 tttattcgct gggaacgccg aacggtcaga aagtaacgat tatgcttgag gagctgctcg
     2281 cgctgggcgt tactggcgca gagtacgatg cctggctgat acgcataggc gatggtgatc
     2341 aattctccag cggctttgtc gaagtgaacc caaattcgaa gatcccggcg ctgcgcgatc
     2401 atacccataa tccgccgatc cgcgtgtttg agtcaggatc gattctgctt tatctggcgg
     2461 agaaatttgg ctacttcctg ccgcaggatc tggcaaaacg gactgaaacg atgaactggc
     2521 tgttctggtt acagggtgct gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt
     2581 acgcgccggt gaaaattgag tacgccatca atcgctttac catggaagcc aagcgccttc
     2641 tcgacgtgct ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat gagtacacca
     2701 ttgcggatat ggcgatttgg ccgtggtttg gcaatgtggt gttaggcggc gtatatgatg
     2761 ccgcagagtt tcttgatgcg ggcagttata agcatgtgca acgctgggca aaagaagtgg
     2821 gcgaacgtcc ggcggtgaaa cgtgggcgta ttgttaaccg caccaacggg ccgctgaacg
     2881 agcagttgca tgagcgccat gacgccagtg attttgagac gaatacggaa gataagcgtc
     2941 aggggtaagg gttagcgttc gtcacagcaa gccatccagg ccggataagg cattcacgcc
     3001 gcatccggca atcgagcaca atgcctgatg cgacgctgcc gcgtcttatc agacctacga
     3061 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
     3121 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
     3181 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
     3241 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
     3301 ctggaacgcc aatacacatt gttaactccg gttattcaac ttcaatactt ttgacgatca
     3361 gcgaatcgcc ggtatcgaca cgcaaccgct cgccatgaca gagcggacac tgcgcatcgt
     3421 gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga
     3481 tgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
     3541 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
     3601 gcttaacatc gtgctgctcc gcctgccgct ggataatttc aacggcgctc tggcaaagcg
     3661 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
     3721 ggtacatccg gattggtgac cggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
     3781 cgttgaccct cttcaatcga catactgtgc gaaagcggtg acatcagcga acaggagagg
     3841 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
     3901 agaccgattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
     3961 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
     4021 aaatcagaaa catacaccgg cattaaagga tgcagaaaag agagatcgtg catcgaacgc
     4081 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     4141 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgccagatt cacgcagcgc
     4201 agccagaacc tgctcaagcg caggttcaat catcgcttcg accgtcggcg tcaagccgat
     4261 gtgcggctcc agtgattccg gaattacgcc gaccagggtc agctttttcg gaaactcgcc
     4321 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     4381 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgcgctctt
     4441 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     4501 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     4561 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     4621 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcagca
     4681 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     4741 tccagcggtt tattcggatc ggcaaccggt gtacccacca gcgactgctc gtaaggaccg
     4801 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac cgcctggtag
     4861 ttgctgatga taccgtcttt aataaccatc cagtgagaga gcataccgcg cggcgcttcg
     4921 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggcttcac aaaggtggtg
     4981 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     5041 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     5101 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     5161 tgggtagatt cgcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     5221 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     5281 ccatcataag ccggaatggt ggtaccttcc cacggtgcct gcggcgcttc gtctttatac
     5341 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     5401 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     5461 ccgtttttac tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     5521 cgcgtcagcc attccgggta gaacgcagca ataactgcgg tatcaacctt ataaacctgt
     5581 tcaacaaagt cgctcagctt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     5641 agcacgccca gaccgtcgag gttgattggg ttagcgaccc cacctaccgc caggttctga
     5701 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     5761 agcgcctgca ggtagtgcgc taccgcgatc aggttcactt ccggcggcag tttcatcgcc
     5821 gggtgacccc agtagccatt ggcgaaaata cccaactgac cgctggcaac cagatctttg
     5881 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     5941 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     6001 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     6061 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     6121 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     6181 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     6241 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     6301 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     6361 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     6421 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     6481 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     6541 ttgcacaacc taacagcgcg ctcagtgccg acaggaacag catgcgggaa tcattacgca
     6601 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     6661 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     6721 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttaacga
     6781 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     6841 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     6901 catagctctg ccacagcgga tgcaccttgt agcccgccga gatcatcagc gaccccattg
     6961 aagactggtg catggtcggc agcagcgcac cgagcgcgat gatgaagaac atcaccttgt
     7021 tcagacgctg tagcgacacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     7081 gagccatcac gccgatgtag atggtcatac agaccgccgt ctcgaacagt accgagttca
     7141 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     7201 tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     7261 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     7321 aggcaaagcc ggtgccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     7381 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     7441 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     7501 gttttggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     7561 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     7621 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     7681 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     7741 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     7801 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     7861 gccatcagct cttcacgcgt accaaaaatc accgcgcccg ccgggcacac ttctacgcag
     7921 ccaggcagac cgcctttatc gagacgttcc acacctttct ggttgcacag ctcgcactta
     7981 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtacgg acaggcgacc
     8041 atgcagtaac ggcagccggt gcacacgtct ttgtcgtaat gaacaatgcc ggttttcgga
     8101 tcttttttca gtgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     8161 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     8221 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     8281 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     8341 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     8401 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     8461 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     8521 tatctttctt ttgctgacga cccagttcac gcaccgccat cacactgaca ccggcaacca
     8581 gcccaatcac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     8641 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     8701 tgcctttatg gaagccgata ccttcttcgt tacagccata gcacgggtga ccaatcgcca
     8761 ccggccacac accgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     8821 gccctttaca gccgaggtgg tacaggcacc agccttcgcg gtggccttca tcaccgaact
     8881 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     8941 ggccataggc gaaggtcgga cggtttttgt catccagttt cggcggtttg ccgtaagtga
     9001 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatgttga
     9061 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     9121 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatggct gctgcgcctt
     9181 ccgccgcttt gcggatgtga tccacaatcg gctcaccggc aaccatgcaa taaataccgt
     9241 tatcttttaa tgggatggaa ccatccacca ctaacacata ctgccctttg tacttctcga
     9301 gagcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     9361 actccagaga gatagtctcc agtacgaggt tttctaccgt tggatgcgtt gcacgaagca
     9421 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     9481 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     9541 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     9601 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     9661 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     9721 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     9781 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     9841 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     9901 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     9961 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
    10021 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
    10081 atggttattc atcgttcacc tcattcgcta tatatcgtca tatatagcga tattttaggc
    10141 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tcccgtaaga
    10201 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
    10261 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
    10321 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
    10381 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
    10441 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
    10501 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
    10561 ctttgcctgt cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
    10621 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
    10681 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
    10741 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc accaccacgg
    10801 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
    10861 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
    10921 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
    10981 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
    11041 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
    11101 ttgcactcct cagctggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
    11161 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
    11221 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
    11281 ttacgggcag atgcagtacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
    11341 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
    11401 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
    11461 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
    11521 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
    11581 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
    11641 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
    11701 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
    11761 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
    11821 gttaattcat caaccttcgt acaatttact aaaccgctgg gtggataaaa gcggcctgct
    11881 ggataccctg caaaataacg gcgtgggctg cattgccttt actcctctgg ctcagggatt
    11941 gctgaccgga aaatatctca acggcattcc tgaagattca cggatgcatc gtgaagggaa
    12001 taaagttcgt ggtctgacgc cgaaaatgct caccgaagcc aacctcaaca gcctgcgctt
    12061 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
    12121 gctgaaagat gagcgagtga cgtcggtatt ggttggtgcc agccgcgcgg agcaacttga
    12181 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagatcga
    12241 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcttccgata aatgacctgt
    12301 taataacggg cgcgagaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
    12361 caagatagcc cattacaaat gcagagagca caaacgtcag atggataatg acgtaccaca
    12421 tcagtttgtt atcagggaca tttttcgcat ccataaagac gcgcagtaag tgaatggaag
    12481 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
    12541 gccagttcag cttctctttg ttctcggaga tatcgagttg tgagacgaaa ttctcataac
    12601 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
    12661 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
    12721 cctggaagaa cttcagcgcc agggcaacta acgcaagcga aaggccaaag tacacggggg
    12781 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
    12841 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcgcgca acagcaaatc
    12901 aacatatccg ttaaaccttt gtcgcataat tctgagggtt agggatcttc ctggttccct
    12961 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
    13021 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    13081 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    13141 atgttggtag cggtcgcctg aaagatcgta aagccctggt gacagggggc gattccggaa
    13201 tcggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    13261 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    13321 aagccgttct gctgccaggc gatttaagcg atgagaaatt tgcccgttcg ctggttcacg
    13381 aagcgcataa ggccttaggt gggctggata ttatggcgct ggtcgccggg aaacaggttg
    13441 ccattcctga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    13501 ttttcgcact gttctggcta acccaggaag cgatccacct gctaccgaaa ggtgcaagta
    13561 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    13621 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc tcggagaaag
    13681 gtattcgggt gaatattgtc gcgccaggcc cgatctggac agcactgcaa atttccggcg
    13741 gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc
    13801 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    13861 ccgcagaagt gcacggcgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    13921 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    13981 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    14041 tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt atccggccta
    14101 caaacgcacg aagattcaac atgttgcaat agattttgta ggcctgatga gcgtagcgca
    14161 tcaggcaatt ctactttact tcgctttggc ggtttcttca ccgaccagac ctatcttcag
    14221 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    14281 gtccgctcgg aagaagatgg tggtgtcttt cttaccttcg gttaacgcat tcaacgccgt
    14341 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    14401 cgacagataa accggttttt ccggacgcgg ctgcggcgtg ctggtagaag caggcaagtt
    14461 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    14521 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    14581 ttcgttaaga tgcattgcca tcggatatta tcctgcacgt aatttttgtg cgacacgaac
    14641 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    14701 cgctgcgaca tcacccagca tcgctttaaa gccgccaatc tgacgcgcga atacgttata
    14761 gataacgacc gccggaatcg ctgccaccag accgattgcc gttgctaaca gagcttctgc
    14821 gatacccggc gcaacgactg ccaggttagt agtctgcgtt tgcgcgatac caataaagct
    14881 gttcatgatg ccccagaccg taccaaacag cccaacgaac ggcgaaatcg cgccgatggt
    14941 tgccagatag ccgttaccgc ggcccatttg acgacctact gcggcgaccc gacgctccag
    15001 acgaaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    15061 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    15121 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    15181 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aggtgactac
    15241 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    15301 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    15361 aagtcacaaa atctgcgcat aataatatca aaacgacgtc gaattgatag tcgttctcat
    15421 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    15481 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    15541 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    15601 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    15661 cggtaaatag cgtgattcag cgcgcttctt cgctggtatt tgagagtatg gaagccaaaa
    15721 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    15781 cccatttttc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    15841 ttccctgcgg ggcggcagcg gttgctaatt cgattcttgc ttttgtcgaa cagggcgatc
    15901 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    15961 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    16021 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc gggttccatc actatggaag
    16081 tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtgccggat gccatcatta
    16141 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    16201 tttctattca agccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    16261 ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa tgcctatctg atgggccaaa
    16321 tggtcgatgc tgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    16381 tgcgtcaaca tcatgaaagc agtctgaaag tggccgaatg gctggcagaa catccgcaag
    16441 ttgcgcgagt taaccaccct gccctgccag gcagtaaagg acacgaattc tggaaacgag
    16501 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aatgatgaag
    16561 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    16621 atgaatcgtt gatcctggca aatcaaccag aacatatcgc cgccattcgc ccacaaggcg
    16681 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    16741 tgattgccga tctggacgcc ggttttgcgc ggattgtata acattgccac ttttggacaa
    16801 ttttgcagac attttattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    16861 ggacgattca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    16921 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    16981 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    17041 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatattggc tggcgcattg
    17101 attgcccagg gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    17161 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    17221 ggctggctgg cacagcttcc tgctaaatat catcagcgcg ccacctgcat gtttgaccgc
    17281 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    17341 accatggcgg gaatttccgg tctgccaaac cgccgtttcc agtttttcaa ctggttaagc
    17401 ggattgctgt gggtcagcgt ggtaaccagt ttcggctatg ccttaagtat gattccgttc
    17461 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    17521 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    17581 cgattttccc tgttcccggt tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    17641 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    17701 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    17761 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    17821 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatattgg cttctgccgc
    17881 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    17941 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    18001 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    18061 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    18121 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    18181 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    18241 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    18301 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgtc cggaaaagag
    18361 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    18421 caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac
    18481 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    18541 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag
    18601 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    18661 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    18721 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    18781 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    18841 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    18901 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    18961 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    19021 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    19081 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    19141 ttcagaatcc aacgccggtg cggtgatctc ccgtaaaacc acaggcgata agcaggcgtt
    19201 ccattcagcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    19261 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacacgg tggaacagta
    19321 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    19381 gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    19441 cgtcatgtgg gcggcgacgc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    19501 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    19561 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgatacca agcgcgctaa
    19621 gctgctgcaa tatgccgaac gcgtctggaa catcactgaa ggttccgacg atgagcgtat
    19681 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    19741 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    19801 cggcatgacc caactgggcg aaaatcatga cattacgctg gatgtcagcc gccgtatata
    19861 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    19921 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    19981 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gttgggactg
    20041 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    20101 ggttatcgct cgattgatac cgccgcggcc tataagaacg aagaaggtgt cggcaaagcc
    20161 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    20221 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactaca gcttgattat
    20281 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    20341 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    20401 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    20461 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    20521 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    20581 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    20641 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    20701 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    20761 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaacg tgcaaattct
    20821 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    20881 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    20941 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    21001 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    21061 acagcccccc tcacagaccg tagaacaaac agtccggcat atttatcaga actataaatc
    21121 agatgccacc gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgtgcgtat
    21181 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggtaatattg gctggctgga
    21241 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgtagcgat
    21301 aaccgaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    21361 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    21421 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    21481 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    21541 tgaacatatt gccgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    21601 ggcggtcaat gccttctata aaaccacctt caagacggcc aacaatcccg atgaagatat
    21661 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    21721 ttcacgcatt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gcattcatat
    21781 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac aacggcgcga
    21841 cggtaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    21901 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    21961 acaataaaaa agggaacact atatgaaaac gattttcacc gtgggagctg ttgttctggc
    22021 aacctgcgtg ctcagtggct gcgtcaatga gcaaaaggtc aatcagctgg cgagcaatgt
    22081 gcaaacatta aatgccaaaa tcgcccggct tgagcaggat atgaaagcac tacgcccaca
    22141 aatctatgct gccaaatccg aagctaacag agccaatacg cgtcttgatg cacaggacta
    22201 ttttgattgc ctgcgctgct tgcgtatgta cgcagaatga taaaaaaatc cccggcagca
    22261 tgtcagttgc cggggatttt ttttaacgtc caaccgccgc tttagggcgt ttcttcgcac
    22321 cagcattcac cggacgagat tgcgtagacg acgctttttt tgccgtagca ggcgtctgac
    22381 gctgggtcgc catcggcgta tgtttcgtca acgctggacg agtattgcgg ttctgacgac
    22441 gagcctcacg catctcttca atggttggcg caggcactaa gcaatcgcga cggctgccaa
    22501 tcagatgctt tttacccatt gcttccagcg cctgacggat taacggccag tttgccggat
    22561 cgtggtaacg caacaacgct ttatgcaaac gacgctgttt gtcacccttc ggtacaaaga
    22621 cgtcttcact cttataacca atcttcgcca gcgggttttt cccggtgtaa tacatggtgg
    22681 tcgagttagc cagcggcgat gggtagaaat tctgtacctg gtcgagacgg aaacgatgct
    22741 ttttcagcca cagcgccaaa ttcaccatat cttcatcacg cgtaccgggg tgcgcagaga
    22801 tgaaatacgg gatcagatac tgctctttac ctgcctgttt cgagtaagta tcgaacagct
    22861 ctttaaagcg gtcatagctg cccatgcccg gcttcatcat cttcgacaac ggcccttctt
    22921 cggtatgttc cggagcaatc ttcagataac cgccgacgtg atgggtcgcc agttctttga
    22981 tatagcgcgg atcttccacg gcgatgtcat aacgtacgcc tgaggcaatc agaatctttt
    23041 taatgccttt cagatcgcga gcgcggcgat agaggttgat cgtcggttcg tggttagtgt
    23101 ccatatgcgg acaaatatcc gggtaaacgc aggacaaacg acggcaggtt tgttcagcgc
    23161 gtggcgattt gcagcgcaac atatacatgt tggcagttgg cccaccgaga tcggaaatta
    23221 cgcccgtaaa acctggaacg gtgtcgcgga tcgcttcgat ctcattaatg atcgaatctt
    23281 cggaacggct ctgaataatg cgcccttcgt gctcggtgat agaacagaaa gagcagccgc
    23341 caaagcagcc gcgcataatg ttgaccgaaa aacggatcat ttcgtaagcc ggaatacggg
    23401 cattgccata ggccggatgt ggcacgcgct tgtatggcag cgcaaaaacg ctgtccatct
    23461 cttcggtaga aagcggaata gcaggcgggt tgatccacac atagcggccg ccgtgttttt
    23521 gcatcaatgc gcgggcacag cctgggttgg tttcgtggtg cagaatacgc gaagcatggg
    23581 cgtacagcac tttatcgccc ttcactttct cgaaagaagg cagcaacacg taggtttttt
    23641 cccacggttt cgggcgcggt ggctgcacgg ttacggcttt ggcttcctgc tttttcggtg
    23701 ccaccggttt gttatccgcg cacggcaaat cttcgccata cggatgcggg atcgggtcga
    23761 ttttcccagg ggtatcaaga cgagtagaat ccacaccgct ccagcctggc aacgcctctt
    23821 ttacgataat cgcagtatta cgcacatcgc ggatttcgct aattggctcg cccatcgcca
    23881 gacggtgcgc cacctccacc agcggacgct caccgttacc aaacatcagc atgtcggctt
    23941 tcgaatccac cagcacggaa cggcgcacgg tatcggacca gtaatcataa tgcgcggtac
    24001 ggcgcagact agcctcaata ccaccgagga tcaccggtac atctttccac gcctctttac
    24061 aacgctgggt ataaaccagt gtggcgcgat ccgggcgctt acccgcgacg ttatctggcg
    24121 tgtaggcatc gtcatgacgt aaacggcgat cggcggtata acggttgatc atcgaatcca
    24181 tgttgccagc agtaacaccg aaaaacagat tcggtttacc cagacgcatg aaatcgtctt
    24241 tgctgctcca gtctggctga gcgatgatcc caacgcgaaa gccctgcgct tccagcatac
    24301 gaccgcaaat cgccatcccg aagcttgggt gatcgacata cgcgtcgcca gtaaccaaaa
    24361 tgatgtcgca gctatcccag ccaagttgat ccatctcttc acgagacatc ggcaaaaacg
    24421 gtgccggtcc aaaacaggcg gcccagtact gcggccatga gaacaggtcg cgatccggtt
    24481 ggatcaggga gatagagctc attttgcttc cagaaatgat aaaaaaataa tcaaaggccg
    24541 gggattataa gccggaacga aagagaaatc gaaaagtatt ccatactcgc cctcctcggg
    24601 cgagtatgaa gattacggta ccggattgac taacagttgc ccaatcgagc cacggtccgc
    24661 catttccagc gtctgactgt tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa
    24721 ataaacaagc agctccactt gtccgtcaac ccaaacggta tctttccagc ctctgtcttc
    24781 cgggaacggc atcgcaccat tcacgttacg gatctggaac atcacgcctt caatatggaa
    24841 cgcttgcggc tcgtccgcgc gtaccgtcca gcgttcccac gttccttgct gcgcggtgac
    24901 atcaatacgg ttgacgtccc acagttgtcc attaataccc gggtcatcac ccagactgat
    24961 atcgcgactg cgaatcggcg aaccggccat gatttcagtt ggcagcaagc gcatcggaag
    25021 actgtctgtg accagcggca gaaggccggt tgggcgcagc gttagcacca gggtagaaac
    25081 cagaatgctg gatggctcaa agaagccacg aatacgatca acaatgctcg ccgcttcgcc
    25141 acaggtgatc gacacttcat caccgttgct catatccacc agaatctcgc ggcgctcgcc
    25201 cggtgccagc gaaagttgct tcaccgatac aggagcaggc aggaatccct gatcgccaga
    25261 aatcacatgt aacgggcgac catcgctcat ctgtagttga tagcgacgag agttcgacgc
    25321 gttcagcagt cgcaagcgca cccagccacg cgagacttca acgtacgggc tttgtacacc
    25381 gttaaccagc agcgtatcac caacaaagcc gccgcttccc ggttcgttgt attctggcgt
    25441 accaaagtta tccagccgtt tatcctgaat aatgaccgga aaatcatcca caccataatg
    25501 gttggggata ggcagcgact tgctgacttc atcttccacc agccacattc cggcaaggcc
    25561 gttatagacc tgctgagccg tgcggttggg agtattggcg tgataccaca gagtagctgc
    25621 gttctggcga atgggcagta cgggtgccca gtcagcgttt ggcgacatca tccgtgccgg
    25681 accgcccatc agcgggcctg gtacctgtag cccggccacc gtcattgaga cattttctgt
    25741 caggcggttg ctgtaaataa gcttaacatc gtcgcccttc cagacgcgga tagtcggccc
    25801 caggtaacga ccattgattc cccagaccga cgcgcgtgtc cctggcgtaa atgaccagtg
    25861 cgcacgttgt acagtcataa acagcggttg cccacggcga gattcaagca gcggcggaac
    25921 gggtagcggt tgctgttgcc cggctgcgct ggccttcagg ggaacagcgc ctgcacaaag
    25981 tgcaatcccc gatgcctgaa tgaactgacg ccgactgagt gacatatttg ctccatgtaa
    26041 aactggctaa agtaataccg tgcaggaaag tgttccccgc aaaaatacag attgccgact
    26101 taaacttttc cggcggcttc gcgttctgcg acttctttat cgagctcggc gattttttgt
    26161 tccattatcg aacgacaatg ggcagccagc tcacgaacct gatctttgcc atactgactg
    26221 acgtcaattg gcggcagcat ttcgacaatc accagaccgt tgtgcagtcg attaagatta
    26281 atcttattcg aagttgtaga gacgcacacg ggaataatcg ggacgcctgc tgcaattgcc
    26341 gcgtgaaatg ctccagtctt gaacggtagc aggccgcgac cacgactgcg ggttccttcc
    26401 gggaacatcc aaatggaaat acggcgtttt ttgaaatgat tcactacttc cgcaatagtg
    26461 ccgtgagctt tagtgcgatt gtttctgtcg atcaataagt tgccggttaa ccagtacaac
    26521 tgcccgaaga aagggatcca cagcaagctc tttttaccta ccgtcaccgt cggcggttgc
    26581 acaatgttcg atgctgtcac catgtcatag ttgttctggt ggttagcgat atagatagca
    26641 ttgccgtagc tttcagcatc tgcaggtttg cggcactcaa ctttcaggcc gaacagcggc
    26701 gcaagacgac caaacatatg cccaaaggtg gccacatgtt tcgggttacg cgggctgaaa
    26761 aggcagtaaa tggagccgaa tacacagact aagatgctgt aaatcacggt aataataaga
    26821 cgaaagatat atagcataac accctctgaa ggtctgagag actggcattg tacgtcacct
    26881 gaaatcaggt tagggcattg ttaaaagccg ctctctggaa aagcgtattg ttaatcgcaa
    26941 cgcatgaata aacaacggtt tcaagggaat ttttatcgtt tccgggggtt gaatacgctg
    27001 ccggatgacg acttaacgtt tcatccggcg ttccttgcaa gcgggaggaa acagcgccct
    27061 ccccggcata ttactcttcg ctatcaccgc tgctggcacg gcgaggagag tcgatctcaa
    27121 cacgatcgat acgctgcaaa ccgcgcatca acgtaccgcg gcgtccacgt tcgccagtga
    27181 ctttctgtaa ctcttccggg cgcagtttaa ttttgcgttt cccaacatga atggtcagcg
    27241 tgctttgcgg cggcagaacg tacagttgcg ccagaccatc ttctccacgc gcggcttctg
    27301 ccgatggaat gttgataatc ttgttgcctt tgcccttcga cagctgcggc agatcgctta
    27361 ccgggaacat caacatacgg cctgcctgag tgattgccag cagcatatcg gaagcatctt
    27421 caattaccac cggcggcata acatgggcat tttccggtaa ggtgatcaaa gccttacctg
    27481 cacggttacg cgccaccaga tcgttaaagg tgcagacgaa accgtaaccc gcatcggaag
    27541 ccatcagcag tttctgatcg tcgctttcca tcagcatatg gtcaacggtc gccccaggcg
    27601 gcaacgttaa tttgccggtg agcggttcgc cctgaccacg cgccgacggc agcgtaatcg
    27661 ggtcaatggc atagctacga ccggtggaat caacaaacac taccggttgg ttgctcttac
    27721 ctttcaccgc cgctttgaag ctatcacccg ctttataatt caggcccggc gcgtcgatat
    27781 catggccttt agcgctgcgt acccagccca tctgcgacag cacaatggtg acaggttcag
    27841 acggcagcat gtcgtgctcg ctcatcgctt tcgcttcttc gcgttcctgc aacggcgaac
    27901 gacgatcgtc accgtaggct tgcgcgtctg cctgcagttc tttcttcagc aggttattca
    27961 ttttacgctc ggaagccaaa atgccctgca actggtcgcg ctctttttcc agttcactct
    28021 gctcaccgcg aatcttcatc tcttccagtt tggcaagatg acgcagtttc agttcgagga
    28081 tcgcttccgc ctgggtttcc gtaaggccaa accgcaacat cagcgccggt ttcggttcat
    28141 cttcattacg aatgatctca atcacttcgt cgatattgag aaacgccacc agcaaacctt
    28201 cgaggatatg caggcgcttg aggactttct ccagacgata gttcagtcgg cggcgcacgg
    28261 tatcgcggcg gaacaccagc cattcggaga gaatttccag caggtttttc accgccggac
    28321 gaccatccag accgatcata ttgaggttaa tgcgatagct cttttccaga tcggtggtag
    28381 cgaagaggtg gttcatcacc tgatccatat ccacgcggtt ggaacgcggc acaatcacca
    28441 gacgggtcgg gttctcgtgg tcagattcat cgcgcagatc gtcaaccatc ggcagctttt
    28501 tgttgcgcat ttgcgcagca atttgctcca gtacgcgcgc acctgaaacc tgatgcggca
    28561 atgcgctgat aaccaccgcg ccatcttctt tcttccacac cgcacgcata cgcactgaac
    28621 cacgtccgtt ctcgtagatt ttacggatct cggcgcgcga agtgataatt tccgcttcag
    28681 tcggataatc cggcccctgc acgatatcca gcagctgatc gagcgtggtt ttcggctggt
    28741 cgattaatgc gattgccgcc tgagccactt cacgcaggtt atgcggtgga atatcggtcg
    28801 ccatgccgac ggcaataccg gtggtgccgt taagcaaaat gtttggcaga cgggcaggta
    28861 gcattttcgg ctcctgcaaa gtgccgtcga agtttggcac ccagtcagcc gtcccctgcc
    28921 ccaactcgct caatagcagc tcggaatatt tcgacaaccg ggattcggtg taacgcattg
    28981 ccgcgaacga tttcggatcg tccggcgcgc cccagttccc ctgaccatca accagcggat
    29041 aacggtaaga gaacggctgc gccatcagga ccatcgcttc ataacaggcg ctatcgccgt
    29101 gcggatggta tttacccagt acgtcaccga cggtacgggc cgatttttta aatttggcgc
    29161 tggcattcag gcccagttca gacatcgcat acacaatgcg gcgctgaaca ggtttcagac
    29221 catcaccaat aaacggcaac gcacggtcca tgatcacgta catggagtag tttaagtagg
    29281 cgttttccgt aaattcatgt agcgcaaggc gctctgccat atcgctcatt aattctgatt
    29341 cctcaactta ttcgccagcc ttcaaagggg caatattgcc ggagatacta cattatctgc
    29401 cgcgtcgagt cacaggagat cagcgtgaat agcgccgccg gacgtgctcc gacggcgata
    29461 aaggcatcaa tgtgccttga tatacaactc tttgctgtag acataatcct ggggattatg
    29521 cagcggaaaa ccgccaacgt agggtttaag cagtttgatt aacggctgat agtagatagg
    29581 aatcagcagt gcctgttggt tgatgatcac ttctgcctgc tgatacaacg cattacgctt
    29641 tgtcgcatca gtgatctgcg tggcctggtt tagtaaggcg tcatactgcg catttttcca
    29701 gtgaccgacg ttttcttcac tatcgctttt gagcgtgttc aggaagctgg aagcatcatt
    29761 gtacgtcgca tcccacgact gccgagacag catgaaatca ccggctcgtc gggcatcaag
    29821 ataggttttc cactccattg tgcgcagcgt cacctgtgca cccagccatt ttttccattc
    29881 ggaagacaac gctatcgcgg tcttttcatg cagatcgtac ttgttgtaga acagctcaaa
    29941 gcgtagcgga tgagaggcgt cgtatcccgc ctgtttcagc aaggcttttg ccatcgcgac
    30001 gcgctcactc attggctttt gcagttcatc gaacgtcgtc gcgctaaagc cttttacctc
    30061 tggcggcgtc agcgtggttg cgggcgttct caaccccagt accttttgcg caataagctg
    30121 tcgatcaacc gtaagatata gcgcccgacg cacccgcaca tcgttaaatg gcggtttctc
    30181 aaggttgaag ttgtaatatt cgctgttcag acgcggaata attcgtagct cgccaggcag
    30241 tgatttttca atggcgggaa tttgctgcgc cggaacccag gtgagatcga cctctcccgc
    30301 gcgatagcgg ttatagccgg tgaccgaatt atccagcgcc agatactcaa cctgttgcaa
    30361 tattgtatgt tgcgcatcgc ggtactttgg atttttgcgt gcagtaatct tttcgttaac
    30421 tacccactga tcaagcacaa aggcaccgtt gtaaaccatg ttctctggct tactccagct
    30481 atcgccatgt ttagcgatga catgatgagg aaccgggaac agcgtcggcc aggcgagcat
    30541 cgtcgtgaac caaggaaccg gctgctcaag cgtaacttca agagtacgat catccgtcgc
    30601 tttgacaccc agcgatgtaa catctgcttt acccgcaaca atagccgcgg cattgttaat
    30661 gtgcgcctgt gccagatagc cagcaaaagg gcttgccgtt ttcgggtcaa ccgcgcgctg
    30721 ccagccgagg acaaaatcct ctgccgtcag aggctgaccg tcggaccact gcaaaccgct
    30781 acgcagatgg aaaatatagc gcctgccgcc gtccagtatc tcccagcgtt cagcctgagc
    30841 gggctgcacc tggccttcac catccatcca taccagacct tcaaacagat ccagcacaat
    30901 ctgcgcggca gtattctcct ccaccttttg cgggtcgagc gtacctgggt cgctatgatt
    30961 gttgtaacga aagacttgtt gcggagcgag cggtgtgttg gtgggaacgt cagcagcgta
    31021 gagaggagcc gcacttacca ggctgaccag ccataataaa tttcgcgtat acatggtatt
    31081 ccttaccgga gaaattataa gcccaacaac aatacgcctg tggcattgtt cgctcaaact
    31141 tatcacgagt gatttggctc acactccggt aaagattccc catcacgcta acggcacata
    31201 aacatccgtt tccagtcgct gctctgtcac cccttcggca agattggtgt agtgaaacag
    31261 aatcggtgct ttacgcattt tctcgccgct tgcaggcagc cagtggcgaa taatgcccca
    31321 tatcgtgtgt gaaatatcgt ccagctcgcc aacgtggcgg gccacggcat aacgtccacc
    31381 ggtaagttca ccattgctca caccataacg attatcggga atcggttcgc taacgctgcc
    31441 gcagatatca aagcgaaacg cttccggtgc ggtggtggca gggtcgtccc aggcgacgcc
    31501 aaaagtctgg ctttggttaa caggagaaag ccccgtctct ttacgccaca tgataaattt
    31561 cgccacactg tagttgagca actcagggct acagcggtgc ggcaacatcg ccacagggat
    31621 tgatgggaaa tcgataatgt ttacatccag tgtcaggttt gtcattttgc ctccctgcga
    31681 aattattccc gggaacgcgt cccgggagcg gtaacaatta cggatttact ttgcggatct
    31741 gtttgacgtc aatttcaaca gaattccagt ctttatcgac ttcaccctga atctcaaccg
    31801 tatctttcgg cgtcaccgtc acgccgttcc agcgtttgtg atcgatatca acattgatag
    31861 taccgctggc atctttgaac acgtagagat cgtcagagat gcgttcaacg atattgccgc
    31921 gcagggttac ccaggtgtcg tcacgcaggg attttgcgct ttctacagtc gttacgctgc
    31981 cgttcggccc ctggaatcct ccggcctgac tttgcgttgc cgatgggcca gaaaaaccgc
    32041 cctgctctgc tgccatcacc ggtgcgctgc acagggccat tactgcgatt actgctgcga
    32101 attttttcat gtttattact ccctttaatg tctgtttccg agcatttaac aagatagtcc
    32161 ttaacaactt cttaagggaa aaaaataaaa tttagtgctg tacagagcgc gttacaacac
    32221 ggtttactgg cagcaaatac ggttatcgca gggatgaaaa aatgcgagtt ttactgatag
    32281 aagatgacat gctgattggc gacggcatca aaacgggcct tagtaaaatg ggttttagcg
    32341 tcgactggtt tacacaaggt cgtcagggaa aagaggcgct ttatagcgca ccttatgatg
    32401 cggtgatcct ggatttaacc ttaccaggca tggatggtcg cgatattttg cgcgaatggc
    32461 gagaaaaagg tcagcgtgag ccggtactga tcctgaccgc gcgcgatgcg ctgggggaac
    32521 gtgtagaagg gctgcgtctg ggagctgacg attatctgtg taaacctttt gcgttgatag
    32581 aagtcgccgc caggctggaa gctctgatgc gccgaaccaa cggccaggcc agcaacgagc
    32641 tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat cgccacgctg gctggcgaac
    32701 ccttaacact gaaaccaaaa gaatttgccc tgctggaatt actgatgcgt aacgctggtc
    32761 gggtactgcc gcgcaaactg attgaagaga aactgtatac ctgggacgaa gaggtcacca
    32821 gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa actcggtagt gattttattc
    32881 gtaccgtgca tggtattggc tacacattag gtgagaaatg aaatttaccc aacgtcttag
    32941 tctgcgcgtc aggctgacgc taatcttttt aattctggcc tcggtgacct ggctgctttc
    33001 cagctttgtc gcctggaaac aaacaacgga taacgtcgat gaattgttcg acacccaact
    33061 gatgcttttt gccaagcggt taagtacgct cgatctcaac gaaatcaacg cggcggatcg
    33121 catggcacag acgccaaata aattaaaaca cggtcatgtt gatgacgatg cgctgacctt
    33181 tgccatcttt acccacgacg gcagaatggt ccttaacgat ggcgataacg gagaagatat
    33241 tccctatagc tatcaacggg aaggttttgc tgacgggcaa ctggtcggtg aagacgatcc
    33301 ctggcgtttt gtctggatga cctcacctga tggcaaatat cgcatcgttg ttggtcagga
    33361 atgggaatac cgtgaagaca tggcgctggc gattgttgcc gggcaattga tcccgtggct
    33421 ggtcgcactg ccgattatgt taatcatcat gatggtacta ctgggtcgtg aactcgcgcc
    33481 gctgaacaag ctggcgctgg cgctacgtat gcgtgaccct gactcggaaa aaccactaaa
    33541 cgcgactggc gtacccagcg aagtgcgacc actggttgag tcgctaaatc aactgttcgt
    33601 ccgcacacat gcgatgatgg ttcgtgaacg acgctttacc tccgacgcag ctcacgaact
    33661 tcgtagcccg ttaacggcgc tgaaagtgca aaccgaagtt gcgcagctct ctgacgatga
    33721 tccgcaggcg cgaaaaaaag cactgcttca attacattac gggatcgatc gcgctactcg
    33781 tctggttgat caactgctca cgctatcgcg gctggactca ctggataacc ttcaggacgt
    33841 cgcggagatc ccgcttgaag atctcctgca atcgtcggtg atggatattt accacacggc
    33901 gcagcaggcg aaaattgacg tgcgactgac actcaatgtc cagggcatca aacgcaccgg
    33961 acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg gataacgccg tgcgttacag
    34021 tccacagggc agcgtggtgg acgtcacgct gaatgccgat aatttcatcg tgagggataa
    34081 cggccccggt gtgacaccag aggcactggc gcgaattggc gaacgcttct atcgcccacc
    34141 cggacaaacc gctaccggca gtggacttgg gctatcgatt gtccagcgaa tcgccaagct
    34201 gcatggcatg aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag
    34261 ctggtaactt ctctgtctat gagagccgtt aaaacggctc tcatagattt tactaatagc
    34321 aaaatataaa ccgtccccaa aaaagccacc aa
//
LOCUS       DAZKFU020000018        33654 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZKFU020000018 DAZKFU020000000
VERSION     DAZKFU020000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.018"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..588
                     /gene="yraH"
                     /locus_tag="ACHOQQ_RS03520"
                     /old_locus_tag="pgaptmp_000704"
     CDS             4..588
                     /gene="yraH"
                     /locus_tag="ACHOQQ_RS03520"
                     /old_locus_tag="pgaptmp_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4469002.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            668..1363
                     /gene="yraI"
                     /locus_tag="ACHOQQ_RS03525"
                     /old_locus_tag="pgaptmp_000705"
     CDS             668..1363
                     /gene="yraI"
                     /locus_tag="ACHOQQ_RS03525"
                     /old_locus_tag="pgaptmp_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4469003.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGLVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1392..3908
                     /gene="yraJ"
                     /locus_tag="ACHOQQ_RS03530"
                     /old_locus_tag="pgaptmp_000706"
     CDS             1392..3908
                     /gene="yraJ"
                     /locus_tag="ACHOQQ_RS03530"
                     /old_locus_tag="pgaptmp_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4469004.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYSYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3919..5010
                     /gene="yraK"
                     /locus_tag="ACHOQQ_RS03535"
                     /old_locus_tag="pgaptmp_000707"
     CDS             3919..5010
                     /gene="yraK"
                     /locus_tag="ACHOQQ_RS03535"
                     /old_locus_tag="pgaptmp_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4469005.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5053..5913)
                     /gene="rsmI"
                     /locus_tag="ACHOQQ_RS03540"
                     /old_locus_tag="pgaptmp_000708"
     CDS             complement(5053..5913)
                     /gene="rsmI"
                     /locus_tag="ACHOQQ_RS03540"
                     /old_locus_tag="pgaptmp_000708"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGU4469006.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5978..8014
                     /gene="lpoA"
                     /locus_tag="ACHOQQ_RS03545"
                     /old_locus_tag="pgaptmp_000709"
     CDS             5978..8014
                     /gene="lpoA"
                     /locus_tag="ACHOQQ_RS03545"
                     /old_locus_tag="pgaptmp_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGU4469007.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7972..8367
                     /gene="yraN"
                     /locus_tag="ACHOQQ_RS03550"
                     /old_locus_tag="pgaptmp_000710"
     CDS             7972..8367
                     /gene="yraN"
                     /locus_tag="ACHOQQ_RS03550"
                     /old_locus_tag="pgaptmp_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGU4469008.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8387..8977
                     /gene="diaA"
                     /locus_tag="ACHOQQ_RS03555"
                     /old_locus_tag="pgaptmp_000711"
     CDS             8387..8977
                     /gene="diaA"
                     /locus_tag="ACHOQQ_RS03555"
                     /old_locus_tag="pgaptmp_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGU4469009.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8987..9562
                     /gene="dolP"
                     /locus_tag="ACHOQQ_RS03560"
                     /old_locus_tag="pgaptmp_000712"
     CDS             8987..9562
                     /gene="dolP"
                     /locus_tag="ACHOQQ_RS03560"
                     /old_locus_tag="pgaptmp_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU4469010.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9676..10716)
                     /gene="yraQ"
                     /locus_tag="ACHOQQ_RS03565"
                     /old_locus_tag="pgaptmp_000713"
     CDS             complement(9676..10716)
                     /gene="yraQ"
                     /locus_tag="ACHOQQ_RS03565"
                     /old_locus_tag="pgaptmp_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4469011.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10789..11424)
                     /gene="yraR"
                     /locus_tag="ACHOQQ_RS03570"
                     /old_locus_tag="pgaptmp_000714"
     CDS             complement(10789..11424)
                     /gene="yraR"
                     /locus_tag="ACHOQQ_RS03570"
                     /old_locus_tag="pgaptmp_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU4469012.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11552..12070
                     /gene="yhbO"
                     /locus_tag="ACHOQQ_RS03575"
                     /old_locus_tag="pgaptmp_000715"
     CDS             11552..12070
                     /gene="yhbO"
                     /locus_tag="ACHOQQ_RS03575"
                     /old_locus_tag="pgaptmp_000715"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGU4469013.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12050..12493)
                     /gene="yhbP"
                     /locus_tag="ACHOQQ_RS03580"
                     /old_locus_tag="pgaptmp_000716"
     CDS             complement(12050..12493)
                     /gene="yhbP"
                     /locus_tag="ACHOQQ_RS03580"
                     /old_locus_tag="pgaptmp_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGU4469014.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12544..12846
                     /gene="yhbQ"
                     /locus_tag="ACHOQQ_RS03585"
                     /old_locus_tag="pgaptmp_000717"
     CDS             12544..12846
                     /gene="yhbQ"
                     /locus_tag="ACHOQQ_RS03585"
                     /old_locus_tag="pgaptmp_000717"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGU4469015.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12833..13336)
                     /gene="yhbS"
                     /locus_tag="ACHOQQ_RS03590"
                     /old_locus_tag="pgaptmp_000718"
     CDS             complement(12833..13336)
                     /gene="yhbS"
                     /locus_tag="ACHOQQ_RS03590"
                     /old_locus_tag="pgaptmp_000718"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4469016.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13330..13854)
                     /gene="ubiT"
                     /locus_tag="ACHOQQ_RS03595"
                     /old_locus_tag="pgaptmp_000719"
     CDS             complement(13330..13854)
                     /gene="ubiT"
                     /locus_tag="ACHOQQ_RS03595"
                     /old_locus_tag="pgaptmp_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGU4469017.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14063..15058
                     /gene="ubiU"
                     /locus_tag="ACHOQQ_RS03600"
                     /old_locus_tag="pgaptmp_000720"
     CDS             14063..15058
                     /gene="ubiU"
                     /locus_tag="ACHOQQ_RS03600"
                     /old_locus_tag="pgaptmp_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGU4469018.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMASNIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15067..15945
                     /gene="ubiV"
                     /locus_tag="ACHOQQ_RS03605"
                     /old_locus_tag="pgaptmp_000721"
     CDS             15067..15945
                     /gene="ubiV"
                     /locus_tag="ACHOQQ_RS03605"
                     /old_locus_tag="pgaptmp_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGU4469019.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16026..17033
                     /gene="yhbW"
                     /locus_tag="ACHOQQ_RS03610"
                     /old_locus_tag="pgaptmp_000722"
     CDS             16026..17033
                     /gene="yhbW"
                     /locus_tag="ACHOQQ_RS03610"
                     /old_locus_tag="pgaptmp_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HGU4469020.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17151..18395)
                     /gene="mtr"
                     /locus_tag="ACHOQQ_RS03615"
                     /old_locus_tag="pgaptmp_000723"
     CDS             complement(17151..18395)
                     /gene="mtr"
                     /locus_tag="ACHOQQ_RS03615"
                     /old_locus_tag="pgaptmp_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGU4469021.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18549..20438)
                     /gene="deaD"
                     /locus_tag="ACHOQQ_RS03620"
                     /old_locus_tag="pgaptmp_000724"
     CDS             complement(18549..20438)
                     /gene="deaD"
                     /locus_tag="ACHOQQ_RS03620"
                     /old_locus_tag="pgaptmp_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGU4469022.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20431..20511)
                     /gene="yrbN"
                     /locus_tag="ACHOQQ_RS03625"
                     /old_locus_tag="pgaptmp_000725"
     CDS             complement(20431..20511)
                     /gene="yrbN"
                     /locus_tag="ACHOQQ_RS03625"
                     /old_locus_tag="pgaptmp_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGU4469023.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20618..21502)
                     /gene="nlpI"
                     /locus_tag="ACHOQQ_RS03630"
                     /old_locus_tag="pgaptmp_000726"
     CDS             complement(20618..21502)
                     /gene="nlpI"
                     /locus_tag="ACHOQQ_RS03630"
                     /old_locus_tag="pgaptmp_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGU4469024.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21611..23746)
                     /gene="pnp"
                     /locus_tag="ACHOQQ_RS03635"
                     /old_locus_tag="pgaptmp_000727"
     CDS             complement(21611..23746)
                     /gene="pnp"
                     /locus_tag="ACHOQQ_RS03635"
                     /old_locus_tag="pgaptmp_000727"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGU4469025.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23993..24262)
                     /gene="rpsO"
                     /locus_tag="ACHOQQ_RS03640"
                     /old_locus_tag="pgaptmp_000728"
     CDS             complement(23993..24262)
                     /gene="rpsO"
                     /locus_tag="ACHOQQ_RS03640"
                     /old_locus_tag="pgaptmp_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGU4469026.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24411..25355)
                     /gene="truB"
                     /locus_tag="ACHOQQ_RS03645"
                     /old_locus_tag="pgaptmp_000729"
     CDS             complement(24411..25355)
                     /gene="truB"
                     /locus_tag="ACHOQQ_RS03645"
                     /old_locus_tag="pgaptmp_000729"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGU4469027.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25355..25756)
                     /gene="rbfA"
                     /locus_tag="ACHOQQ_RS03650"
                     /old_locus_tag="pgaptmp_000730"
     CDS             complement(25355..25756)
                     /gene="rbfA"
                     /locus_tag="ACHOQQ_RS03650"
                     /old_locus_tag="pgaptmp_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGU4469028.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25921..28593)
                     /gene="infB"
                     /locus_tag="ACHOQQ_RS03655"
                     /old_locus_tag="pgaptmp_000731"
     CDS             complement(25921..28593)
                     /gene="infB"
                     /locus_tag="ACHOQQ_RS03655"
                     /old_locus_tag="pgaptmp_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGU4469029.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIESESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28618..30105)
                     /gene="nusA"
                     /locus_tag="ACHOQQ_RS03660"
                     /old_locus_tag="pgaptmp_000732"
     CDS             complement(28618..30105)
                     /gene="nusA"
                     /locus_tag="ACHOQQ_RS03660"
                     /old_locus_tag="pgaptmp_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGU4469030.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30133..30585)
                     /gene="rimP"
                     /locus_tag="ACHOQQ_RS03665"
                     /old_locus_tag="pgaptmp_000733"
     CDS             complement(30133..30585)
                     /gene="rimP"
                     /locus_tag="ACHOQQ_RS03665"
                     /old_locus_tag="pgaptmp_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGU4469031.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30792..30868)
                     /locus_tag="ACHOQQ_RS03670"
                     /old_locus_tag="pgaptmp_000734"
     tRNA            complement(30792..30868)
                     /locus_tag="ACHOQQ_RS03670"
                     /old_locus_tag="pgaptmp_000734"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30832..30834),aa:Met,seq:cat)
     gene            31216..32559
                     /gene="argG"
                     /locus_tag="ACHOQQ_RS03675"
                     /old_locus_tag="pgaptmp_000735"
     CDS             31216..32559
                     /gene="argG"
                     /locus_tag="ACHOQQ_RS03675"
                     /old_locus_tag="pgaptmp_000735"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGU4469032.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVDNLENKGQ"
     gene            complement(32567..>33654)
                     /locus_tag="ACHOQQ_RS03680"
                     /old_locus_tag="pgaptmp_000736"
     CDS             complement(32567..>33654)
                     /locus_tag="ACHOQQ_RS03680"
                     /old_locus_tag="pgaptmp_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4469033.1"
                     /translation="FATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT         8268 a         9058 c         8344 g         7984 t
ORIGIN      
        1 taaatgaata aagttacaaa aacagctatt gcgggcttac ttgcgctttt cgcaggtaat
       61 gccgctgcaa ccgatggtga aatagttttt gatggcgaga ttttaaaatc cgcttgtgaa
      121 atcaatgact ctgataagaa aattgaagtt gctcttggtc actataatgc cgaacagttt
      181 cgttctgtag gcgatcgtag cccaaaaatt ccattcacta ttcctttagt taactgccca
      241 gtgactgggt gggagcacga taacggcaac gttgaagcgt ctttccgttt atggctggaa
      301 acgcgggata atggtaccgt ccctaacttc cctaacttgg caaaagtggg ctcttttgct
      361 ggcacagcag caacaggtgt gggtatccgt attgacgatg cggaaagcgg aaaccttatg
      421 ccactaaatg ctatgggcaa tgataacaca gtttatcaga tccctgcgga ctccgctgga
      481 attgtcaatg ttgacctcat cgcttactac gtatcaaccg ttgaagcatc cgaaatcacc
      541 ccaggggaag cagacgctgt cgttaacgta acgctggatt accgttaata cgttacggcg
      601 ttatctgacc tttcagataa cgcccttttc cttcctcttt ctcgttgtat caggttgaaa
      661 aataactatg tcaaaacgaa cattcgcggt gataataacc ttgttgtgta gcttctgtat
      721 cggccaggcg cttgcagggg gtatcgtttt acagcgaacg cgagtgatct atgatgccag
      781 ccgcaaagag gctgcgttac ctgtcgcaaa caaaggcgca gaaacgcctt atttactgca
      841 atcatgggta gataatatag atggtacaag ccgtgcccca tttattataa ccccaccgct
      901 atttcgtctt gaagctggcg atgattcatc actgcgaatt attaaaacag ctgataacct
      961 gcctgaaaat aaagagtcgc tgttctacat taatgttcga gccattccag caaagaaaaa
     1021 atcagatgat gttaatgcta acgagttgac gctggtattt aaaacacgga tcaaaatgtt
     1081 ttatcgcccc gcacacttga aggggttggt caacgatgcg tggaaatcac tggaatttaa
     1141 acgtagtgac cattcactca atatatataa cccaactgaa tattacgtcg tatttgccgg
     1201 actggcagtc gataaagccg atctcacaag caaaattgaa tatatcgcgc ccggagaaca
     1261 taaacagtta ccacttccag catctggcgg aaagaacgtg aaatgggctg cgatcaatga
     1321 ttatggcggc agttccggga cagaaactcg tccactgcaa taacaaatat aaaaacacag
     1381 gtcatcaggg aatgccacaa cgacaccacc agggacataa acgcacaccg aaacagttgg
     1441 cgctcattat caaacgctgt ttgccgatgg tgctcactgg cagcggcatg ctttgcacta
     1501 ccgctaacgc cgaagagtat tatttcgacc ccattatgct ggaaaccaca aaaagtggta
     1561 tgcaaacaac cgatctgtca cgtttttcaa aaaaatacgc acaactacca ggaacttatc
     1621 aggttgatat ctggctgaat aaaaagaagg tttcacagaa aaaaattaca tttaccgcca
     1681 atgcagagca acttctgcag ccacagttta cggtagaaca actacgtgag ctgggtatta
     1741 aggtggatga aatcccggcg ctggctgaaa aagatgacga tagcgtgatc aactcgcttg
     1801 aacaaatcat tcccggtaca gctgctgaat ttgatttcaa tcatcagcga cttaatttga
     1861 gcattcccca aattgcactg taccgtgatg caagaggtta cgtctcccct tctcgttggg
     1921 acgatggtat accaacgctg tttaccaact actcgtttac aggttctgat aaccgttacc
     1981 gccagggcaa tcgtagccaa cgacagtacc tgaatatgca aaatggtgct aattttggcc
     2041 cctggcgatt acgtaactat tctacgtgga cacgcaacga tcaggcgtca agctggaaca
     2101 ctatcagtag ttatttacaa cgtgatatca aggcgttgaa gtctcagttg cttctgggag
     2161 aaagcgccac cagcggcagt attttttcca gctacacctt tactggcgtg caactcgctt
     2221 ccgacgataa tatgttgcca aacagccagc gcggatttgc cccaacggta cgcggtatcg
     2281 caaacagtag tgcaatcgtg actatcaggc aaaatggtta tgtgatctat caaagcaacg
     2341 tgccagcggg tgcctttgaa attaacgatc tctacccctc ttccaacagc ggcgatttag
     2401 aagtcacgat tgaagaaagt gacggtacac aacgtcgctt tatccagcct tattcttcat
     2461 tacccatgat gcagcgacct gggcatctaa aatatagcgc gaccgctgga cgctatcgcg
     2521 ctgatgcaaa cagtgatagc aaggaacccg aatttgctga agccacggca atatatggtt
     2581 tgaataatac ttttacgctg tatggcggcc tgctcggttc tgaagattat tatgcgctgg
     2641 ggatcggtat cggcggcaca cttggcgcac tgggcgcgtt gtcgatggat atcaacagag
     2701 ctgacaccca attcgataac cagcactctt ttcatggcta tcaatggcgt acgcagtaca
     2761 tcaaagatat cccggaaacc aacaccaata tcgctgtcag ctactatagc tataccaacg
     2821 atggctattt tagttttgat gaagccaata cccgcaattg ggactataac agtcgccaaa
     2881 aaagtgaaat tcaattcaac atcagccaga caatatttga tggggtaagt ctgtatgcct
     2941 ccggttcaca gcaagactat tggggcaata acgagaaaaa caggaatatc tctgttgggg
     3001 tttccggcca gcaatgggga attggttaca gcctgaatta tcaatacagc cgctacactg
     3061 atcaaaataa tgaccgcgca ctctctttga atctcagtat tccgttagaa cgctggttac
     3121 cgcgtagccg ggtttcctat cagatgacca gccagaaaga tcgcccaacc caacatgaaa
     3181 tgcgtcttga tggctcactg ctggatgatg gtcgcctgag ctatagcctg gaacaaagtc
     3241 tggatgacga taacaaccat aacagtagcc tgaacgccag ttaccgttca ccttatggaa
     3301 ccttcagtgc cggatacagt tacggtaatg acagcagcca atacaattac ggcgttaccg
     3361 gcggcgtggt tatccatcct catggcgtga cgctctcgca atatctgggc aacgcttttg
     3421 cgcttatcga tgctaacggg gcatctggcg tgaggataca aaactatccg gggattgcta
     3481 ccgatccctt tggctatgca gtggttcctt atctcacaac ttatcaggaa aaccgtctct
     3541 cggtagatac tacgcagctg cccgataacg tcgatcttga acaaacaaca cagtttgtgg
     3601 tgcccaacag aggtgcaatg gtagcggcgc gtttcaacgc caatatcggt tatcgcgtac
     3661 ttgttacagt cagcgatcgc aacggtaaac cgttgccctt tggcgctctt gccagcaacg
     3721 atgatacggg gcaacaaagt atcgtcgatg agggcggcat actatatctc tctgggatat
     3781 cgagtaaatc acaaagctgg actgtacgct ggggaaatca ggcagatcaa caatgtcagt
     3841 ttgcttttag tacaccggat tcagaaccaa caacctctgt attacaaggc acagcgcagt
     3901 gccattaagg ataaaaaaat gaaaagagcg cctcttataa caggactttt gttgatatcc
     3961 acatcctgcg cttatgcctc ctcaggaggg tgtggagccg acagcactag cggtgcgaca
     4021 aattacagca gtgtggttga tgatgttacg gtgaaccaga cagataacgt gacaggacgg
     4081 gagtttacct ctgcaacgct aagtagcact aactggcaat acgcctgttc ctgctctgcg
     4141 ggtaaggcag ttaaacttgt ctatatggtc agccccgtac ttaccaccac tggacatcag
     4201 acaggatatt acaaactcaa tgacagcctg gatattaaaa ccacattaca ggcaaacgac
     4261 attccaggac tcacaaccga ccaggttgtc tctgttaaca cccgattcac acagataaaa
     4321 agcagcaccg tatattctgc tgcaacccaa acgggtgttt gccagggtga cacgtctcgt
     4381 tatggacccg ttaatattgg tgcaaatacc acctttaccc tgtatgtcac caagccattt
     4441 ctcggctcga tgaccattcc gaaaacggat attgccgtca ttaaaggcgc gtgggtcgat
     4501 ggaatgggaa gcccgtctac aggtgacttc catgatttag tcaagttatc gatacaggga
     4561 aatctcaccg ccccacagtc gtgcaaaatt aatcagggcg atgttattaa ggttaatttt
     4621 ggattcatca atggtcagaa gtttaccacc cgcaatgcca tgccagacgg ttttactcca
     4681 gtagactttg atatcactta tgactgtggt gatacttcaa agattaaaaa ctcgttgcaa
     4741 atgcgcatcg acggtacaac tggggtagta gaccagtaca acctggtcgc cagacgaaga
     4801 agttcagaca atgcgcccga tgtcggtatt cgtattgaaa atctcggtgg cggagttgca
     4861 aatattcctt ttcagaacgg tatccttccc gttgatcctt ccgggcatgg caccgtcaat
     4921 atgcgcgcct ggccagttaa tctggtcggt ggtgagctgg aaacaggaaa atttcagggc
     4981 acagccacca ttaccgtcat cgtgcggtaa aagaagacgt tgcgttttga ttaacgcaac
     5041 gcttcggcac tcttacccct gctgctccag cgcatactta tatagcgcat ttttcttcac
     5101 gccgtgaatt tctgcggcca gcgccgccgc ttttttcagc ggcagttctg cctgtagcag
     5161 cgccagcgtg cgcagggcat cggcgggtaa gtcttcttcc tgtgctttat gaccttcgac
     5221 aatcagcacc atttcgcctt tgcgacggtt ttcatcttcc tttacccacg ccagcagctc
     5281 gccaacgggc gcgccgtgaa tggtttccca ggttttggtc agctcacgcg ccagaaccac
     5341 gtagcgggat tcgcctaata ccgcaacgat atcttccagg ctatctaaca gacggtgggt
     5401 agattcataa aaaatcagcg tgcgcggctc cgcttcaatg gcttttagcg catcacggcg
     5461 gccttttgat ttggcaggta aaaagccttc gtaacagaaa cggtcagagg gtaaacccgc
     5521 ggcgcttaac gcagtgatag cagcacacgg tccgggtagc ggcaccacac gaatccccgc
     5581 ttcacggcag gtacgcacca gatggtagcc aggatcgtta attagcggcg ttccggcatc
     5641 ggaaaccagc gcaatgtttt gcccctcttg cagcttcgcc agcagcgttt cggctttttg
     5701 ttgttcgtta tggtcgtgca gcgcaaacaa ccgggcatta atcccaaaat gttgcagcaa
     5761 taaaccggtg tgacgagtat cctcggcggc aatcagatca acggcctgta atacctctaa
     5821 cgcacgctgg gtgatatccg ccagattgcc gattggcgtc ggtacaatgt aaagctggcc
     5881 ctgagaatta tccgccgatt ggtgttgttt cattgtgtcg tccgtattgc cgatttaata
     5941 ttgagcattg cgtaaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg
     6001 tttgaaagcc gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg tcggttgtgg
     6061 cacccatact cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc
     6121 cttttatctt cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact
     6181 cgccattcgt gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca
     6241 actaccgcaa gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat
     6301 taaactggcg cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa ttacacccgc
     6361 cgatttagaa caaaaccagc aagcccgtta ctggcaggca aaaatcgatg ccagccaggg
     6421 gcgtccttcc attgatttac tgcgcgcgtt aattgctcag gaaccgctgc ttggcgcgaa
     6481 agaaaaacag cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca
     6541 ggcgaatacg ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca
     6601 gcgcgtctgg tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg
     6661 gcagaaacgt tatccgaaca acccgggcgc gaaaatgctg ccaacgcagt tggttaacgt
     6721 aaaagcgttt aaaccagcct cgaccaacaa aatcgccctg ctgttgccac tgaatggcca
     6781 ggcagcggta tttggtcgca ctattcagca aggctttgaa gcggcgaaaa atatcggcac
     6841 tcagccagtg gcagctcagg tagctgccgc acctgccgca gacgtagcag aacaacccca
     6901 accgcaaacc gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg
     6961 tgaacagcct gcagcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt
     7021 aagcgcaccc gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact
     7081 tagccagatc ttaagccagg ttcagcagga tggcgcgagt attgtggtcg gtccgttgct
     7141 gaaaaataac gttgaagagt tgctgaagag caacactccg ctgaacgtac tggcactgaa
     7201 ccagccggag aatatcgaaa atcgcgtcaa tatttgttac ttcgcgcttt caccggaaga
     7261 cgaagcgcgc gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct
     7321 gatcccacgc agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa
     7381 actgggcggc ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg
     7441 tgttaacggc ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac
     7501 cgactccggc atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt
     7561 caccaataat ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc
     7621 ttttatcaaa ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa cgctgtacgc
     7681 cagctcccgc agtgcgcaag ggaccgctgg cccggatttc cgactggaga tggaaggctt
     7741 gcagtacagc gaaatcccga tgctggcagg cggtaatcta ccgttaatgc agcaggcact
     7801 cagcgcggtg aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg
     7861 gtcgctggca aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa
     7921 taccggaagc ctgacggcta acccggattg cgtgattaac aggaagttat catggctaca
     7981 gtaccaacaa ggtcaggtag tccccgccag ttaaccacca aacagaccgg cgatgcgtgg
     8041 gaagtacaag cgcgtcgctg gctggaaggc aaaggactgc gatttgtcgc cgctaacgtg
     8101 aacgagcgtg gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc
     8161 gaggtgcgtt accgccgttc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc
     8221 aaacaacaca aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt
     8281 gatactgtgg attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt
     8341 aaggatgcct ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat
     8401 taaagcttgc ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga
     8461 tgccatctcc cgtgcggcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct
     8521 ctgttgtggt aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa
     8581 ccgtttcgaa acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt
     8641 cttaacggcg attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc
     8701 gctgggtcat gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat
     8761 tgtaaaagcg gtagaagctg ccgttacgcg tgatatgacc attgtggcat tgaccggcta
     8821 tgacggcggt gaacttgcag gtttgttagg gccacaggat gtggagatcc gcatcccttc
     8881 gcatcgcagt gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct
     8941 gatcgataac acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc
     9001 gccaatcgca gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt
     9061 gggtaccgct gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt
     9121 ggacgatggt accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa
     9181 gaaagaagcg cgcattaatg taacggccta tcagggcaaa gtgctgctgg ttgggcagtc
     9241 accaaacgct gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa
     9301 cgaagtgtat aacgagattc gtcagggcca gccgattggt ctgggcgaag cgtctaatga
     9361 tacgtggatc accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc
     9421 caacgtgaaa gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg
     9481 tgaagcgaaa gcggcggcag atattgccag ccgggtgagc ggcgtgaagc gggtaactac
     9541 ggcgtttacg tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc
     9601 ttatcaggcc tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac
     9661 atgttttccc tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc
     9721 caccatcgcc cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag
     9781 tgacggcaaa ctcaccgccg ggagcgtcat caacaatgcc agcgccggag cagttcccat
     9841 acctgccagc atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa
     9901 gcatcctgct accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc
     9961 atgggggaat aaccagacgc gagcggcacc caacaccagt actgcaagga tgtaaaccgg
    10021 gatcgtactc cagaaaagcg tccatagcgc cctgccccag cggctaaaaa atccgccctg
    10081 tgcttccggt atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt
    10141 ttgcaccagc gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc
    10201 aaaaccccag ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt
    10261 gcccatccag aatgccagcg caccgcccat cgacacctgt tggcgacgca ttcccgccgc
    10321 gaccggagcc gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag
    10381 cgtgccgcga aagcgcgatt gcccaagcgt acgcaacaac cagtcacgcg ggatcagcac
    10441 ctgaatcaac gaaccgagga tcacccccag cactgccgct ttccataccg cgaggaaata
    10501 gatcatcgcg taatccaacg ccgcttgcca cggattagca tccgcctgcg caaggataga
    10561 tttaccgata ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt
    10621 cacataccag agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat
    10681 gggcgttgcc gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg
    10741 ataattgagc gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct
    10801 tttgcgtaat tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc
    10861 tgccagcatg acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggtaacaa
    10921 acggaacagc ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagtaa
    10981 catcgacggg cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat
    11041 ctcccctttg acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac
    11101 caccagcata tgctgcgcgc ccagccgccg cccggttaat gcggtatcca ctaccagcgt
    11161 gtaatcggca tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca
    11221 acaaaacaca atgtcgatgg gatcggtgac ctgcgccagc gcgtcagtca gttgcggatc
    11281 atggggatta aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctattgc
    11341 gttaactttc ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc
    11401 gcctgtaatc agtacctgac tcatcttcgc tcctttacag aattgtctac cttgcgctcc
    11461 acggctcatg caccaggctt aatagaccat gaggtaatta tccccgattg tgggaaattc
    11521 gcctcatcca atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcac
    11581 tgatgaattt gaagattcag aatttacttc acccgcagac gagttccgta aagccggaca
    11641 cgaagtgatt accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc
    11701 cagcgtgacc atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgtt
    11761 gctaccgggc ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac
    11821 ccgtgatttt gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct
    11881 gatcagcgcc gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga
    11941 tgttaaaaat gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct
    12001 ggttaccagc cggacgccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct
    12061 cggtgcctga gccgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga
    12121 atttgatttc atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa
    12181 agcgacgatt gtacgccttg cgcgcgaggt cgctttcctc accttccagc ctgcggatct
    12241 cacctttaaa ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc
    12301 cggcaaccgc agcctgcggc ccactcatct gcgcatgacg cgttttttct tccgtcaaaa
    12361 tgtagaaggc gactttctgc gcatcaaaca gataaaaagc attagcgcac cagagttccc
    12421 cttcctgctg cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga
    12481 tgagtgtttc cattgctgtt ctcttttata ctgtgggcct gaccttaaca tgcgacgcag
    12541 acgatgacac cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg
    12601 atcaccacgg atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca
    12661 ctgcgcggga aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg
    12721 gcgttacggg cggaatatcg cgttaaacaa ctgaccaaac ggcaaaaaga gcgtctggta
    12781 gcggaaggcg cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc
    12841 gattgaaatg ctcgtgatac tcaaccagac cagttacgcc attcaacgcg tcatccgcta
    12901 aacgatgtac ctggaaggcg ctttcagtgc ctggccagcg gcaacgcaga tcgtgatgtg
    12961 ccgccagttc aaagccaaaa cggctgtaca gcgccggatc gcccagcgtc accactgcgg
    13021 catagccgaa ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct
    13081 gtccacggta tttttcatct accgccaacg gtgccatgcc gacccattgc aggtcttcgc
    13141 cctgcacatc aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg
    13201 ccaccagccc cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg
    13261 catcactttc gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt
    13321 ctactcgaat tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat
    13381 tcccgcctca acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg
    13441 ctccagttca atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc
    13501 aatcaccagc cgacgctgga agaagagcgt atccggatcc tgtttacgcg ccgcaatcat
    13561 cagcagatcg ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac
    13621 cagtttgcca ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact
    13681 taaccagcgg ccttcaagaa actccagctc gccatcatcc agcgcctgac ggaattgcca
    13741 gcttaagacc tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac
    13801 actcaacaga gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt
    13861 tactccctgt ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata
    13921 aatcaacaat tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc
    13981 aaaaattgtc gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgat
    14041 agcgcaacct tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct
    14101 taaggcggcc atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgataccaa
    14161 tgcccgtcac ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt
    14221 tgtccatcaa catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg
    14281 ttacgcccgt tggcagcgtg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat
    14341 cctcgccgac ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca
    14401 tgtgtcagtg caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt
    14461 tgacgttgcc cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc
    14521 acgggtcaca cctgtcccgc tggaagtgtt tgctttcggc agcctgtgca ttatgtcgga
    14581 aggtcgttgc tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg
    14641 ttctccggcc cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa
    14701 cgaagtgctg atcgatcgtt atcaggacgg cgaaaacgca ggttatccga cgttgtgtaa
    14761 agggcgttat ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa
    14821 taccctggaa ctgctaccgg agttaatggc gtcgaatatc gcttcggtga aaattgaagg
    14881 ccgccagcgt agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga
    14941 ccgttgtaag gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg
    15001 gtcgatgtcc gaaggcacgc aaaccaccct tggcgcgtat caccgtaaat ggcagtgaga
    15061 agagcaatga aatattcctt agggccagtg ctgtggtact ggccaaaaga gacgctggaa
    15121 gatttttatc agcaggccgc caccagcagc gccgacgtga tttatcttgg cgaagcggta
    15181 tgcagcaagc gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc
    15241 ggaagtggta agcagatagt gctctccacg ctggcgctgg tgcaggcatc atctgaactg
    15301 ggcgaactga aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc
    15361 gtggtgaata tgtgcgctga acgcaaactg ccgttcgtcg ccggacacgc gctgaactgc
    15421 tataacgcgg tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg
    15481 gtggagcttt cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gctgggcatt
    15541 cgcaaccagt ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgct
    15601 cgctgcttta ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt
    15661 aagtatccga acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat
    15721 ggcattcaga ccatgagcgg ctacgtttac aacctcggta acgagctggc atccatgcag
    15781 ggcctggttg atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac
    15841 gccttccgcg ctaatgaaaa tggcgctgca ccactgccgt tgacggcgaa cagcgactgt
    15901 aacggctact ggcggcggct ggcaggactg gagctgcaag cctaagtaaa tagctcactt
    15961 tgttaacaac tttaactact ctttaatgca gtattaaaga ttaatcggta acaaagtgag
    16021 ctgttatgac tgataaaacc attgcgtttt cgctactcga tctggccccc attcccgaag
    16081 gttcttcagc gcgagaagca ttctcccact ctctcgatct cgcccgtctg gctgaaaagc
    16141 gcggctatca tcgctactgg ctggcagaac accacaatat gactggcatt gccagtgctg
    16201 ccacgtcggt attgattggc tatctggcgg cgaataccac cacgctgcat ctggggtctg
    16261 gcggcgtgat gttgcctaac cactcaccgt tggtcattgc agaacagttc ggcacgctca
    16321 atacactcta tccggggcga atcgatttgg ggctgggtcg tgctccgggt agtgaccagc
    16381 gaaccatgat ggcgctacgt cgtcatatga gcggcgatat tgataatttc ccccgggatg
    16441 tcgcggagct ggtggactgg tttgacgccc gcgatcccaa tccgcatgtg cgcccggtac
    16501 caggctatgg cgagaaaatc cccgtgtggt tgttaggctc cagcctttac agcgcgcaac
    16561 tggcggcgca gcttggtctg ccgtttgcgt ttgcctcaca cttcgcgccg gatatgctgt
    16621 tccaggcgct gcatctttat cgcagcaact tcaaaccgtc agcacggctg gaaaaaccat
    16681 acgcgatggt gtgcatcaat attatcgccg ccgacagcaa ccgcgacgct gaatttctgt
    16741 ttacctcaat gcagcaagcc tttgtgaagc tgcgccgtgg cgaaaccggg caactgccgc
    16801 cgccgattca aaatatggat cagttctggt caccgtctga gcagtatggc gtgcagcagg
    16861 cgctgagtat gtcgttggta ggtgataaag cgaaagtgcg tcatggcttg cagtcgatcc
    16921 tgcgcgaaac cgacgccgat gagattatgg tcaacgggca gattttcgac caccaggcgc
    16981 ggctgcattc gtttgagctg gcgatggatg ttaaggaaga gttgttggga tagtgtgtct
    17041 taacgcggga agccttatcc gagctggcaa cgctgtccta catagacctg ataagcgaag
    17101 cgcatcaggc attgtgtagg cagcagaaat gtcggataag gcaccgctga ttactgatac
    17161 accggcagta aattaaagct cgataaaata tgcaccagtg cgttgccgac gccaaacacc
    17221 agaatcagcg caatcatcgg cttgccaccc cagacgcgga atttcgggct gccaaagcgt
    17281 ttacgcgatg cgcgggccag cagtgccgga acaattgccg cccagatggt agccgctaaa
    17341 ccagcataac caatggcgta caggaatccg ttcgggaaca gcaggccccc cacgacaggc
    17401 ggggcaaagg tcagcaatgc tgttttcaag cggcccacag ctgagtcatc gaaaccaaac
    17461 agatctgcca gatagtcaaa caaacccagc gttacgccaa ggaacgaact cgctaccgca
    17521 aagtttgaga acacgaccag cagcagatcc agactacggc tgttcagtac gccgcttaac
    17581 gcctgtacca gcacatcaat attaccgccc ttctctgcaa taccgataaa ctccggacgc
    17641 gggatgttac ccatcgtcgc cagcaaccag atggtataca gcgccagcgc catcagcgta
    17701 ccgtacacca ggcatttcac gatggttttc ggatctttgc cgtaatactt catcaggctt
    17761 ggcacgttac cgtgataacc aaacgatgcc agacagaacg gcagggtcat caacagatac
    17821 ggtgcataag acgcattgct ttcggcgacg ttgaacaatg tcgcaggctg cacatgcccc
    17881 agcaggctac caaaggtgag gaagaaggta atgactttcg cccccagcac aatcgctgtc
    17941 atgcgactga cggctttagt gctcaaccac accacaaacg ctaccaacaa tgcaaaacca
    18001 aaacccgccg cccgtgccgg gacgtttagt gacatctctg cgaaggtgtg atgcagaatc
    18061 gaaccactgg cagaaatata ggcataggtc aggatataga gcacaaaggc aatggaaatg
    18121 ccgttgacca cgttccagcc tttgcccagc aaatctttgg tgatggtgtc aaaactcgaa
    18181 ccgattctgt aattcaggtt agcttccaga atcatcaagc cggaatgcag catacagaac
    18241 caggtaaaga tcagcgccgc cattgaccag aaaaaccacg ccccggacat gaccactggc
    18301 agagaaaaca tccctgcgcc aataatggtg ccgccgataa tcaccacgcc gccaagcagc
    18361 gacggtgacg tttgggtggt ggttagtgtt gccatgaggg cttctctcca gtgaaaaata
    18421 gtgcgactgc gttgttatgt attgcactgt accagtacac gagtacaaaa gacagaaaaa
    18481 aagccccgat ggttcaaatc ggggctgtat atattatttt acagactgtg ttcgctgttc
    18541 agcgatgatt acgcatcacc accaaaacga cgacgaccgg tagagtcatc acgacgcgga
    18601 gcgcggcctt cacgacgttc gccgctaaaa cgacgaccat caccacgacc accttcacgg
    18661 cgttcaccgc tgaagttacg gccgccttca cgacgctcgc cactgaaacc acgaccaccg
    18721 ccacgacgct caccgccagt atgcggctgt gcatcgccca gtaattgcat attcatcggc
    18781 ttgttcagaa tgcgagtacg tgtaaagtgt tgcagcactt cacccggcat acctttcggc
    18841 agttcgatgg tggagtgaga agcaaacagc ttaatgttac cgatgtaacg gctgctgatg
    18901 tcgccttcgt tagcaatcgc accaacgata tgacgaactt caacaccatc atcgcggccc
    18961 acttcaatac gatacaactg catgtcacca acatcacgac gttcacgacg cggacgatct
    19021 tcacggtcgc cacgcgggcc acggtcgtta cgatcgcgcg gaccacggtc atcacggtca
    19081 cggaattcac gtttcggacg catcggcgca tctggcggaa caatcagagt acgttcaccc
    19141 tgtgccattt tcagcagtgc ggcggccaga gtttcgagat ccagctcttc accttctgca
    19201 gtcggctgaa ttttgctcag cagtgcgcgg tattgatcca gatcgctgct ttccagctgc
    19261 tgctgtactt tagcggcgaa tttttccaga cggcgtttgc ccagcagttc tgcgttcggc
    19321 agttctactt ccggaatagt cagcttcata gtacgttcaa tgttgcgcag cagacgacgc
    19381 tcgcggttct caacgaacag cagcgcgcgg ccagcacgac ccgcacgacc ggtacgaccg
    19441 atacggtgaa cgtaagactc agaatccatc gggatatcgt agttaaccac caggctgata
    19501 cgctcaacgt ccaggccacg ggctgcaacg tcggtcgcaa tcaggatgtc cagacgacca
    19561 tctttcaggc gttccagtgt ctgctcacgc agcgcctggt tcatgtcacc gttcagcgcg
    19621 gcgctgttgt agccgttacg ctcaagagct tcagccactt ccagagtcgc atttttggta
    19681 cgaacgaaga taatcgccgc atcaaaatct tccgcttcca ggaaacgtac cagtgcttcg
    19741 tttttacgca taccccagac agtccagtag ctctggctga tgtcaggacg ggtagtcacg
    19801 ctggactgaa tgcgcacttc ctgcggctct ttcataaagc ggcgggtaat gcgacgaatc
    19861 gcttccggca tggttgcaga gaacagagcg gtctgatgac cttccgggat ctgcgccata
    19921 atggtttcaa cgtcttcgat gaagcccatg cgcagcattt cgtcagcttc atccagaacc
    19981 agaccgctca gtttagagag gtccagagtg ccacgtttca ggtggtccag cagacggccc
    20041 ggagtaccga caacgatctg cggcccctga cgcagggcgc gtaattgcac gtcataacgc
    20101 tggccgccgt acagggcaac cacatttacg ccgcgcatgt gtttagagaa atccgtcatt
    20161 gcttcagcaa cctgtaccgc cagttcgcgg gtcggtgcca gcaccagaat ctgtggtgct
    20221 ttcagctcag gatcaagatt ctgcagcaga ggtaaagaga atgctgcagt ttttccgctc
    20281 cccgtctggg ccatacccag aacgtcgcgg ccattcagca gatgtggaat acactccgcc
    20341 tgaattggag atggtttttc gtaacccaga tcgttaaggg cttcaaggat aggagccttc
    20401 aggcccagat ctgcaaaagt ggtttcgaat tcagccatgt agtacgtgtg cctcaaaatt
    20461 aatggcggcc agtctacata actcatcatg aaattgatca gcaattttca ttgaaaagtg
    20521 tgaaccggct caaagtaggt gtattaacga acaacaacgc cctcacccgt taaggtgatg
    20581 gcaatcaaaa aagattacgg gctgatgtgt acgtcagcta ttgctggtcc gattctgcca
    20641 ggtcatcttg gtcctggccc aggagcgata attccaacaa tgcgtatcgg tgctcaacaa
    20701 agttatgaac gttgttggca accgccagtt tgaacagtgc cgtggcgctg tccaaatccc
    20761 ccagacttag gtagtactta cctaaataga agttggtttc actgagatgc tcagcgagcg
    20821 aggtgttatc cgttgcgtcc gccttgagcc tttccattaa cgtttgttcg ctaatgttgc
    20881 ccaggtagaa ctcgacaatg ttccatcccc actgttcctt atccgatttt tcgaagtgct
    20941 gtttcaacac ttctttagcc tgcttctcat cgagcttctg ctcggcgaga taaagccaca
    21001 gactacggaa aggatcattg ggatcgtctt gataaaacgc cagcagatca tcttgcgcta
    21061 acttgtcacg accgccgtaa tataatgcga tcccgcgatt caagtgcgcg tagttgtaag
    21121 ttggatcaag ctcaagtaca gaatcaaacg cttcataggc agcatcaaaa ttgcctgcct
    21181 gcgttaaata tatgcctaag taattgaata cttcaggcat atccggtcgg attgccagcg
    21241 cttgcgaaaa atcgttacgc gctaatgccc tcagaccgag actatcatac aacactccgc
    21301 gctcatataa aagctgtgcg cgttcgtcat cggttaaagc ccgactggca aggatttgtt
    21361 ccatacgtgc cagaatcact tcctgctgta aagtcggttg caatggtacc gcgaggactt
    21421 cacttttacg ccaggaagta ttactgcatc ctgcaagcgt aagtgctgtc gcaacgaaac
    21481 accagcgcaa aaaaggcttc atttcccact cccgaagacc acggttgaat gaacgtcctg
    21541 ttcccggttg ctaacaaggc gtcctgcccg gttaaaagcc ccccgccgca gcggagggca
    21601 aatggcaacc ttactcgccc tgttcagcag ccggagcttc cggtgctgca gcaggttgag
    21661 actgctcagt cgcttcttta atgctcagac ggatacggcc ctggcgatca acttccagaa
    21721 ctttcaccgg tacttcctga cccatctgca ggtaatcggt cactttctca acgcgtttgt
    21781 cagcgatttg agagatgtgt accagacctt ctttaccgcc gccgatggca acaaatgcgc
    21841 cgaagtcaac gatacgggtc actttaccag tgtagacgcg gcccacttcg atttctgcag
    21901 tgatctcttc gatacgacga atagcgtgtt tcgctttctc gccgtcggtc gctgcgatct
    21961 tcacagtacc gtcatcttcg atttcgatgg tggtgccggt ttcttcggtc agggcacgga
    22021 taacagagcc gcctttaccg ataacatctt tgatcttgtc cgggttgatc ttgatggtat
    22081 ggatacgcgg tgcgaactca gagatatcgc cacgcggcgc gttgatcgcc tgttccatta
    22141 cacccaggat gtgcagacgc gcacctttag cctggttcag tgcaacctgc atgatctctt
    22201 tggtgatacc ttcaattttg atatccatct gcagtgcaga gataccgtcg cgggaacccg
    22261 caactttgaa gtccatatcg cccaggtgat cttcgtcgcc caaaatgtca gacagtacaa
    22321 cgtagttgtc gccttctttc accagaccca ttgcgatacc cgcaacggca gctttgattg
    22381 gcacacctgc gtccatcagc gccagagacg caccgcacac ggaagccata gaagaagaac
    22441 cgttggattc agtgatttca gacacaacac gtacggtgta cgggaatttg tccatatccg
    22501 gcattactgc cagcacgccg cgcttcgcca gacgaccgtg accaatttca cgacgcttcg
    22561 gagaaccgac catgccggtt tcgcctacgg agtacggagg gaagttgtag tggaacagga
    22621 aggtatcggt acgttcgccc atcagttcat caagaacctg cgcgtcacga gcagtaccca
    22681 gcgttgcggt aaccagcgcc tgcgtttcac cacgggtgaa cagcgcagaa ccgtgagtac
    22741 gcggcagcac gccagtacgc acatccagac cacggatcat atctttttca cgaccatcga
    22801 tacgcggttc gcctgccagt acgcggctac gaacaacgtt tttctcgatc gcgtgcagaa
    22861 tttcacccag ttcgttttcg tccagggttt cgtcttcagc aagcagcgtc gcgatggttt
    22921 cagatttgat gacatcaacc tgcgcataac gctcttgttt gtcggtgatg cggtaagcat
    22981 cgctcagacg agcttcagcc agtgcagcaa cgcgcgcgtt cagcgcttcg tttaccggct
    23041 ccggctgcca gtcccaacgc ggtttaccgg cttctttcac cagttcattg atgttctgaa
    23101 taacaacctg ctgttgttca tgaccgaaca ctactgcgcc cagcatctgg tcttcgctca
    23161 gcagttcagc ttcagattca accatcagta cagcggcttc agtacccgca acaaccagat
    23221 ccagtttgct ctctttcagc tcgtcctgag tcgggttcag tacgtactgg tcattgatgt
    23281 aacctacgcg ggcagcacca atcgggccat tgaacggaat accagacaga gacagtgctg
    23341 cggaagcacc aatcatcgcg acgatatccg ggttaacctg cgggttaaca gaaaccacgg
    23401 tggcgataac ctgaacttcg ttgacgaagc cttccgggaa cagcgggcga atcgggcgat
    23461 caatcagacg cgcgatcagg gtttcgcctt cgcttggacg accttcacga cggaagaagc
    23521 tacccgggat acgaccagca gcgtaggtac gctcctgata gttaacggtc agtgggaaga
    23581 agtcctgacc tggtttggct tttttctggc caacaacggt aacaaatacc gcggtgtcat
    23641 ccatgctaac cataacagcg gcagtagcct gacgagccat catgccggtt tccagagtca
    23701 cggtgtgttg gccgtactgg aatttacgaa cgatcggatt aagcaatgta atatcctttc
    23761 tctttcttag acagtacctt acggcactgg tgttaatacc cgatcttctg cgcatcctcg
    23821 cgactaatga caaccctaac ccagctctat gtgggtaaag cctctcatta gccgcgcgaa
    23881 cctctgcaac ggaagatcat tcatagcaac aatacattag tttccagtga attgctgccg
    23941 tcagcttgaa aaaaggggcc actcaggccc ccttttctga aactcgcaag aattagcgac
    24001 gcagacccag gcgctcgatg agctgggtgt aacgtgctac gtctttacgt ttcaggtagt
    24061 cgagcagttt acgacgctga gaaaccatgc gcagcagacc acgacggctg tggtgatctt
    24121 ttttgtgctc tgcaaagtgg ccctgcaggt ggttgatctg tgcagtcagc agtgctacct
    24181 gaacttcggt agaaccggtg tcgtttgcgt cacgaccaaa ctcagaaacg attttagctg
    24241 ttgcttcagt acttagagac attttaaaac tccaaagtat atagaatgaa aggacgccga
    24301 tctctaattc agcgatccca gtgtacgtta cgcaaagtgt taaacaattt acgcgacgtt
    24361 aagcggcagt attctactcg tagcgacctg ttatcgcaag acggttaaca ttacgccggg
    24421 tattcaacca ccagacgacg aggcgcaacg cggccttcat cgtcaatttc gcccataccg
    24481 ataaatttgc cgttctcacc ttccgtgacg cgaaccagtc cttccagcgg cgcgccagat
    24541 gtacgaaccg ggttaccatt tttgaagtaa acagaagacg ttaacggaag attcaccacc
    24601 ggatagtccg aagctggact gtccattggc atcagtaatg gatcaagtaa ctccgcagct
    24661 ggaatatcct gctgttcggc ttgttcgacc agttcacgca gatgttccag ggtcaccatc
    24721 cgttcaaccg gatatttact taccgccaga cggcgcaggt aaataacatg cgcgccacaa
    24781 ccgagtttct cacccaggtc gtcaatgata gtgcggatat aagtgccttt tgagcagtga
    24841 atttccagct ccagctcatt gccttcatgg cgaataaaca gcaattcata aacggtaatc
    24901 ggacgcgctt cacgcggaac ttcaatgccc tgacgcgcat attcgtacag ttttttgccc
    24961 tggtatttca gtgctgaata catcgatggg atctgttcga tatcgccacg gaaagtatcc
    25021 agtgccgccg ccagttgttc tgcactaaag gttaccggac gctcttcaac gatttgtccg
    25081 tcggcgtcag aagtatcagt acgctgtcca agacgcgcaa tgacccgata gcgtttgtcg
    25141 gagtccagca gatactggga aaacttcgtc gcttccccga ggcaaatcgg caacatgccg
    25201 gtcgccagcg ggtccagcgc accggtatga ccggcacggt tggcgttata aatccgtttc
    25261 actttttgca gcgcatcgtt gctggacata ccctgaggtt tatccagcaa caaaacgccg
    25321 ttaatgtcgc gaccgcgacg acgaggacga ctcattagtc ctccttgctg tcgtccgggt
    25381 taacacgacg ttcttcgtca tgtttgacca cgctggtcac caggtttgac atgcgcatcc
    25441 cttcaaccag agagttgtcg tagaagaagg tcagttccgg cacgatacgc aggcgcatcg
    25501 ctttccccag caggctgcgg atgaaaccag aagcttcctg caacgctttg atgcccgctt
    25561 taactgcgtc ttcatctttg tcgttgagga aagtgacata cactttggca tacgccaggt
    25621 cgcgagacat ttcgacaccg gaaacggtgg tcatcatgcc cagacgagga tctttaattt
    25681 cacgctgcag gatgagagcg atctcttttt gcatttcctg cgctacgcgc tgcgggcgac
    25741 caaattcttt cgccataata aattctcctg acaaaaaagg ggctgttagc ccctttttaa
    25801 aaattaattt caggtggaag ggctgttcac gttggcctga taagacgcgc tagcgtcgca
    25861 acaggcaatc catgccggat gcggcgtaaa cgccttatcc cgcatagaac cctaaaaacc
    25921 ttaagcgatg gtacgttgga tctcgatgat ttcgaatact tcgatcacat cgccagtgcg
    25981 gacgtcgttg tagttcttaa cgccgatacc acattccata ccgttacgga cttcgttaac
    26041 gtcatctttg aagcggcgca gggactccag ctcgccttca tagataacca cgttgtcacg
    26101 cagaacgcgg atcgggttgt gacgtttaac cacaccttcg gtaaccatac agcctgcgat
    26161 ggcaccaaat ttcggcgatt tgaacacgtc acgaacttcc gccagaccga taatctgctg
    26221 tttcagttcc ggagacagca taccgctcat cgccgctttc acttcgtcga tcaggttata
    26281 gatgacggag tagtaacgta gatccaggct ttcggattca atcactttac gtgcagaggc
    26341 atcagcacgt acgttgaagc caaccaggat ggcgttggat gccgcagcca gggtggcgtc
    26401 ggtttcggtg ataccaccta cgccagaacc gatgatcttc actttaactt cgtcagtaga
    26461 cagtttcagc aaggagtcgg agatcgcttc cacagaaccc tgtacgtctg ccttcaggac
    26521 gatattcact tcgtgaactt cgccttcggt catgttggcg aacatgttct cgagtttaga
    26581 tttctgctga cgcgccagtt taacttcgcg gaatttaccc tgacgataga gtgcaacttc
    26641 acgcgctttc ttctcgtcac gtacaacggt aacttcatca cccgcagccg gaacaccgga
    26701 caagccgagg atttccaccg gaatggacgg acccgcttcc agcacttcct gacccagttc
    26761 gttacgcatc gcacgaacac gaccgtattc gaagccacac agaacgatat cgcccttgtg
    26821 cagagtacct tcacgtacca gaacggtagc aaccggacca cgacctttat cgaggaagga
    26881 ttcgataacc gcaccgctcg ccataccttt acgtaccgct ttcagctcca gaacttccgc
    26941 ctgcagcagg atagcgtcca gcagttcatc gataccggta cccgctttcg cagatacgtg
    27001 tacgaactgg ctttcaccgc cccactcttc cggcaggatg ccgtactggg agagttcgtt
    27061 cttaacgcga tccggatcgg cttctggttt atcgatcttg ttcactgcaa ccaccaccgg
    27121 tacctgcgcc gctttcgcgt gctggattgc ttcgatggtc tgcggcatta cgccgtcgtc
    27181 ggcagcaaca accaggacta cgatgtcagt tgcctgcgca ccacgtgcac gcattgaagt
    27241 aaacgcggcg tgccccgggg tgtccaggaa ggtgatcatg ccgttttcag tttcaacgtg
    27301 gtatgcacca atgtgctggg taatgccgcc cgcttcgcca gaggccactt tcgttgaacg
    27361 aatgtagtcc agcagagagg ttttaccgtg gtcaacgtga cccatgatgg tcacaaccgg
    27421 agcgcgcggt tcagccgcag cacccgtgtc acggtcgctc attaccgcct cttccagctc
    27481 gttttcacga cgcaggataa ctttgtggcc catctcttca gcaaccagct gtgcggtttc
    27541 ctgatcgata acctggttga tggttgccat tgcgcccagt ttcatcatcg ctttgatgac
    27601 ctgagagcct ttaaccgcca tcttgttcgc cagttcgcca acggtgatag tttcgccgat
    27661 cacaacgtca cggttaacgg cctgagcagg cttctggaag ccttgctgca gcgaagaacc
    27721 tttacgtttt ccgcccttac cgccacgtac tgctgcgcgt gcttcttcac gatcagcttt
    27781 tgattcagcg tgtttgttgc ctttcttcgg acgcgctgct ttcgcgttac gaccacggcc
    27841 acggccgcct tcgacttcac gatcgctttc gtcttctgcc tggcgagcat gttgagaagt
    27901 agtgacgtga taatcgctgg aatcctcagt cggttccgcg ttatcagtcc atttgttttc
    27961 ttccgccata cgacgtgctt cttcagcaac gcgacgtgct tcttcttcga gtttacgacg
    28021 cgcttcttct tcggctttac gcttgagctc tgcagcttcc tgctcacggc gggctttttc
    28081 agcctgggcg tttttagtca tatcgtcttg ttgattgctc actttgtctt tttccgcagc
    28141 ttcacgtttc gcttgttcag cagcttcacg cttagcttgt tctgcggcct cacgttcagc
    28201 tttttgttgc gcctcgcgtt tagccgattc ttctgcctca cgacgggctt gctcttccgc
    28261 ttcacgctgc gcttgctctt ccgctgcaag gcgttcagcc tcttgcggat cgcgtttcac
    28321 aaaggtgcgt ttcttgcgga cttcgatttg taccgatttg ctttttccac cggtaccagg
    28381 aatgttaagg gtgctgcgtg ttttacgttg cagcgtcaat ttgtccgggc ctgaattttt
    28441 ctgattcagg tggtcaatca aagtctgttt ctcttgtgca gacacagagt cgtcagcaga
    28501 cttccggata cctgcatcag caaattgctg taccaggcgt tccacggagg tctgtcgctc
    28561 tgcggccagc gttttaatcg ttacatctgt catgctgttc cttcctgcta cagtttatta
    28621 cgcttcgtca ccgaaccagc aaatattacg ggcagccata atcagtgctc cggctttttc
    28681 gtcggtcaac ccttcgatat cagccagatc atcaatgccc tgttcggcga gatcttccag
    28741 cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca cgatctaccc cttcaaggtt
    28801 cagcagatcg tcagccggtt tgttatcacc gaggctttct tcctgggcct gtgcaatggt
    28861 ggccagtgca tttttagcac gctcgcgcag tgcttcaacg gtcggctcat caaggccttc
    28921 gatttccaac agctctttca tcggcacata ggccagttct tccagcgtcg agaagccttc
    28981 ctctaccaga acagtcgcga agtcttcgtc gatgtcgaga tatttggtga aggtgtcgat
    29041 cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg tcaacggtca tcacgttgag
    29101 ttcccaaccg ctcagctgcg aagccagacg cacgttctga ccgttacggc caatcgcctg
    29161 cgccaggtta ccggcttcaa cggcgatatc catggtgtgt ttatcttcat ccaccacgat
    29221 agaagcaacg tctgctggtg ccattgcgtt aatcacaaac tgcgccgggt tatcatccca
    29281 caggacgata tcgatacgct cgccgcccag ttcagtagaa accgcctgaa cacgcgcgcc
    29341 acgcatacct acgcaagcac ctaccggatc gatacgttta tcgttggttt tcaccgcgat
    29401 tttcgcacga gaacccgggt cgcgagccgc tgctttaatt tcaatcactt cttcgccgat
    29461 ttctggcact tcaatacgga acagttcgat cagcatttcc ggcttggaac gagtgacgaa
    29521 cagttgcgcg ccacgcgctt ccgggcgaac ggaataaagc acgccacgaa cgcggtcgcc
    29581 agggcggaag ttttcacgcg gcagcatatc ttcgcgcagg atcacggctt cagcgttgtt
    29641 gcccagatcc agagagatgt tgtcgcggtt tacttttttc accacgccgg tgatgatttc
    29701 accttcgtgt tcacggaact gatcaaccac catcgcacgt tcggcttcac gcactttctg
    29761 cacgataacc tgttttgccg tctgggtagt gatacggtca aaggtaacag actcaatctg
    29821 atcttcaacg taatcgccca ggttcaggct ttcatcttca taacgtgcgg cttcaagggt
    29881 gatttccttg gtcggctggg tgacttcatc aacaactaac cagcgacgga aggtgtcaaa
    29941 atcaccgctt ttgcgatcga tctgtacgcg gacgtcgatc tcttgttcat attttttctt
    30001 tgttgctgtc gccagcgcgc tttccaatgc ttcgaaaatc ttctcgcgag gtagcgcctt
    30061 ttcattggat acggcttcaa ctacagccaa aatttctttg ttcatcgcgg gcttttcacc
    30121 tcatccagac tattaaaagt ggggaaccag gttcgccttc tggatattac tcagcgcgaa
    30181 cacttcatct ttaccttcga cggtaactgt gatcatttca ccgtctaccg ctttgataac
    30241 gccctgccat ttacgacggt tttgtaccgc catacggaga accagagtca cctcttctcc
    30301 gacaaaacgg gcgtagtgtt cagccgtgaa cagtgggcga tcgagacccg gtgaggagac
    30361 ttccaggtta taagcaacgg tgatgggatc ttcaacatcc agcacagcac ttacctggtg
    30421 gctcacatca gcacaatcat caacattgat gccatcttca ctatcaatat agatgcgcag
    30481 tgtggatgtg cgaccgcgaa taaattcgat gccaaccagt tcaaaaccca gggcctcaac
    30541 tggcgcagta atcatctctg ttaatttttg ctctaatgtg gacaagccca cccccaagac
    30601 ataaaaaaag ggcctaaagc ccagttattc tgtagtcaga taacaaaaaa ccccgataaa
    30661 tcggggcttt atataactga accctataac cgcaactgcg gtctggagca ctttccagaa
    30721 ggattttttc aaatcccact acgaaggccg aagtcttcac agtatatttg aaaaaggact
    30781 ctaagggaaa gtggttgcgg gggccggatt tgaaccgacg accttcgggt tatgagcccg
    30841 acgagctacc aggctgctcc accccgcgcc tgaaacgtgg caaattctac tcgttttggg
    30901 taaaaaatgc aaatactgct gggatttggt gtaccgagac gggacgtaaa atctgcaggc
    30961 attatagtga tccacgccac attttgtcaa cgtttattgc taatcacgtg aatgaatatc
    31021 cagttcactt tcatttgttg aatacttttg ccttctcctg atccccctta agcgcattat
    31081 tttacaaaaa acacactaaa ctcttcctgt ctctgataaa agatgattaa atgaaaactc
    31141 atttattttg cataaaaatt caatgagagc ggaaatccag gctcatcatc agttaattaa
    31201 gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca acgtattggt
    31261 atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg acaaaaggga
    31321 gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga ttatgatgcg
    31381 atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga ctgccgcaaa
    31441 caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa caccaccggt
    31501 ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggcac catgctggtt
    31561 gctgctatga aagaagatgg cgtgaatatc tggggtgacg gcagcaccta taaaggaaac
    31621 gatatcgaac gtttctatcg ttacggtctg ctgaccaatg ctgaactgca gatttacaaa
    31681 ccgtggcttg atactgactt tattgatgaa ctgggcggtc gtcatgaaat gtctgaattt
    31741 atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcttactc cacggactcc
    31801 aacatgcttg gtgcaacgca tgaagcgaag gatctggaat acctcaactc cagcgtcaaa
    31861 atcgtcaacc cgattatggg cgtgaagttc tgggatgaga gcgtgaagat cccggcagaa
    31921 gaagtcacag tacgctttga gcaaggtcat ccggtggcgc tgaacggtaa aacctttagc
    31981 gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg tctgggcatg
    32041 agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta cgaggctccg
    32101 gggatggcgc tgctgcacat tgcgtatgaa cgtctgctta ccggtattca caacgaagac
    32161 accattgaac agtatcatgc acatggtcgt cagttgggcc gtctgctgta ccagggacgt
    32221 tggtttgatt cccaggcgct gatgctgcgt gactctctgc aacgctgggt tgccagccag
    32281 atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat cctgaatacc
    32341 gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg cgactcggtg
    32401 ttctcgccag atgatcgtat tggtcaattg accatgcgta acctggatat caccgatacc
    32461 cgcgagaaac ttttcggtta tgccaaaacc ggcctgcttt cctcctctgc cgcttcaggc
    32521 gtgccgcagg tggataatct ggaaaacaaa ggccagtaat tcgacatcaa ccctgccctt
    32581 caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc acataatcga
    32641 aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca atcacttcct
    32701 caagcgtttg cggctgttcc ggctttgtta cccccatcca cgcattaata aggtaattat
    32761 tgtaagctgt cgaaaagatg ttgttttccg ttgtgcgatc cacaccatcc ccaagaacgg
    32821 ggctatacca gataaacatc ggaacatgat atgcctgctg gctggcctcc ctgcctccat
    32881 gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca aaatacatga
    32941 ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta tctgtgtaat
    33001 gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg gcgctggatt
    33061 gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca atcagtttct
    33121 tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat tcatcaaatc
    33181 ctctgacata aactgtttcc atggcacgca tggcgatact ggtaactgct gtaccattct
    33241 gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga ttagccatat
    33301 taataatgtt gtcggggtaa ttatgaatgt catggctcaa aacagaatca gcagttaaag
    33361 aaaggggaac cgacagcgca gtgtaaggtg caccgcttat tgcttgatta aacagtttga
    33421 tctgttttct ttgtgcttcg acttgcggtg tcgtagagcg tgtatatcca tacaaagaca
    33481 tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata cctgtatccc
    33541 tgactgataa ttgaaaatac ggcactgtgt ttgcaattga gagtaatctt tgatgctctt
    33601 tcgctgctaa agcaaaataa ttgagattga aaaacggcgt ataggtagca aatc
//
LOCUS       DAZKFU020000019        33640 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZKFU020000019 DAZKFU020000000
VERSION     DAZKFU020000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.019"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            19..255
                     /gene="ydhI"
                     /locus_tag="ACHOQQ_RS03685"
                     /old_locus_tag="pgaptmp_000737"
     CDS             19..255
                     /gene="ydhI"
                     /locus_tag="ACHOQQ_RS03685"
                     /old_locus_tag="pgaptmp_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGU4469034.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            258..1115
                     /gene="ydhJ"
                     /locus_tag="ACHOQQ_RS03690"
                     /old_locus_tag="pgaptmp_000738"
     CDS             258..1115
                     /gene="ydhJ"
                     /locus_tag="ACHOQQ_RS03690"
                     /old_locus_tag="pgaptmp_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU4469035.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVFSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1115..3127
                     /gene="ydhK"
                     /locus_tag="ACHOQQ_RS03695"
                     /old_locus_tag="pgaptmp_000739"
     CDS             1115..3127
                     /gene="ydhK"
                     /locus_tag="ACHOQQ_RS03695"
                     /old_locus_tag="pgaptmp_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4469036.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3128..3649)
                     /gene="sodC"
                     /locus_tag="ACHOQQ_RS03700"
                     /old_locus_tag="pgaptmp_000740"
     CDS             complement(3128..3649)
                     /gene="sodC"
                     /locus_tag="ACHOQQ_RS03700"
                     /old_locus_tag="pgaptmp_000740"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU4469037.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3730..4626)
                     /gene="ydhF"
                     /locus_tag="ACHOQQ_RS03705"
                     /old_locus_tag="pgaptmp_000741"
     CDS             complement(3730..4626)
                     /gene="ydhF"
                     /locus_tag="ACHOQQ_RS03705"
                     /old_locus_tag="pgaptmp_000741"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGU4469038.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4675..4914)
                     /gene="ydhL"
                     /locus_tag="ACHOQQ_RS03710"
                     /old_locus_tag="pgaptmp_000742"
     CDS             complement(4675..4914)
                     /gene="ydhL"
                     /locus_tag="ACHOQQ_RS03710"
                     /old_locus_tag="pgaptmp_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGU4469039.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5017..5616
                     /gene="nemR"
                     /locus_tag="ACHOQQ_RS03715"
                     /old_locus_tag="pgaptmp_000743"
     CDS             5017..5616
                     /gene="nemR"
                     /locus_tag="ACHOQQ_RS03715"
                     /old_locus_tag="pgaptmp_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGU4469040.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRNSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKTIIATSAV"
     gene            5654..6751
                     /gene="nemA"
                     /locus_tag="ACHOQQ_RS03720"
                     /old_locus_tag="pgaptmp_000744"
     CDS             5654..6751
                     /gene="nemA"
                     /locus_tag="ACHOQQ_RS03720"
                     /old_locus_tag="pgaptmp_000744"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGU4469041.1"
                     /translation="MSSEKLYSPLQVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSQEQIAAWKKITAGVHAENGHIAVQL
                     WHTGRISHASLQPDGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRTRFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     TRLQRKAGLNPQRAESFYGGGAEGYTDYPTL"
     gene            6832..7239
                     /gene="gloA"
                     /locus_tag="ACHOQQ_RS03725"
                     /old_locus_tag="pgaptmp_000745"
     CDS             6832..7239
                     /gene="gloA"
                     /locus_tag="ACHOQQ_RS03725"
                     /old_locus_tag="pgaptmp_000745"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGU4469042.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7342..7989
                     /gene="rnt"
                     /locus_tag="ACHOQQ_RS03730"
                     /old_locus_tag="pgaptmp_000746"
     CDS             7342..7989
                     /gene="rnt"
                     /locus_tag="ACHOQQ_RS03730"
                     /old_locus_tag="pgaptmp_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGU4469043.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8082..12698
                     /gene="lhr"
                     /locus_tag="ACHOQQ_RS03735"
                     /old_locus_tag="pgaptmp_000747"
     CDS             8082..12698
                     /gene="lhr"
                     /locus_tag="ACHOQQ_RS03735"
                     /old_locus_tag="pgaptmp_000747"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGU4469044.1"
                     /translation="MADNPNLSSPLPDVFSPATRDWFLSAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDVAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRATPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILSARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRTSQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPAMISGQERLACMDDAARLRDALGVQLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(12749..13096)
                     /gene="grxD"
                     /locus_tag="ACHOQQ_RS03740"
                     /old_locus_tag="pgaptmp_000748"
     CDS             complement(12749..13096)
                     /gene="grxD"
                     /locus_tag="ACHOQQ_RS03740"
                     /old_locus_tag="pgaptmp_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGU4469045.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13431..14258
                     /gene="mepH"
                     /locus_tag="ACHOQQ_RS03745"
                     /old_locus_tag="pgaptmp_000749"
     CDS             13431..14258
                     /gene="mepH"
                     /locus_tag="ACHOQQ_RS03745"
                     /old_locus_tag="pgaptmp_000749"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGU4469046.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14386..14967
                     /gene="sodB"
                     /locus_tag="ACHOQQ_RS03750"
                     /old_locus_tag="pgaptmp_000750"
     CDS             14386..14967
                     /gene="sodB"
                     /locus_tag="ACHOQQ_RS03750"
                     /old_locus_tag="pgaptmp_000750"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGU4469047.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15113..16282)
                     /gene="ydhP"
                     /locus_tag="ACHOQQ_RS03755"
                     /old_locus_tag="pgaptmp_000751"
     CDS             complement(15113..16282)
                     /gene="ydhP"
                     /locus_tag="ACHOQQ_RS03755"
                     /old_locus_tag="pgaptmp_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4469048.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16448..16537)
                     /gene="cydH"
                     /locus_tag="ACHOQQ_RS03760"
                     /old_locus_tag="pgaptmp_000752"
                     /gene_synonym="ynhF"
     CDS             complement(16448..16537)
                     /gene="cydH"
                     /locus_tag="ACHOQQ_RS03760"
                     /old_locus_tag="pgaptmp_000752"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGU4469049.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16836..17861
                     /gene="purR"
                     /locus_tag="ACHOQQ_RS03765"
                     /old_locus_tag="pgaptmp_000753"
     CDS             16836..17861
                     /gene="purR"
                     /locus_tag="ACHOQQ_RS03765"
                     /old_locus_tag="pgaptmp_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGU4469050.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17858..18790)
                     /gene="punR"
                     /locus_tag="ACHOQQ_RS03770"
                     /old_locus_tag="pgaptmp_000754"
     CDS             complement(17858..18790)
                     /gene="punR"
                     /locus_tag="ACHOQQ_RS03770"
                     /old_locus_tag="pgaptmp_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGU4469051.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            18903..20114
                     /gene="punC"
                     /locus_tag="ACHOQQ_RS03775"
                     /old_locus_tag="pgaptmp_000755"
     CDS             18903..20114
                     /gene="punC"
                     /locus_tag="ACHOQQ_RS03775"
                     /old_locus_tag="pgaptmp_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGU4469052.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            20405..21553
                     /gene="cfa"
                     /locus_tag="ACHOQQ_RS03780"
                     /old_locus_tag="pgaptmp_000756"
     CDS             20405..21553
                     /gene="cfa"
                     /locus_tag="ACHOQQ_RS03780"
                     /old_locus_tag="pgaptmp_000756"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGU4469053.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21593..22234)
                     /gene="ribE"
                     /locus_tag="ACHOQQ_RS03785"
                     /old_locus_tag="pgaptmp_000757"
     CDS             complement(21593..22234)
                     /gene="ribE"
                     /locus_tag="ACHOQQ_RS03785"
                     /old_locus_tag="pgaptmp_000757"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGU4469054.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22449..23822
                     /gene="mdtK"
                     /locus_tag="ACHOQQ_RS03790"
                     /old_locus_tag="pgaptmp_000758"
     CDS             22449..23822
                     /gene="mdtK"
                     /locus_tag="ACHOQQ_RS03790"
                     /old_locus_tag="pgaptmp_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGU4469055.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(23863..25119)
                     /gene="ydhQ"
                     /locus_tag="ACHOQQ_RS03795"
                     /old_locus_tag="pgaptmp_000759"
     CDS             complement(23863..25119)
                     /gene="ydhQ"
                     /locus_tag="ACHOQQ_RS03795"
                     /old_locus_tag="pgaptmp_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4469056.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIIENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25427..25503
                     /locus_tag="ACHOQQ_RS03800"
                     /old_locus_tag="pgaptmp_000760"
     tRNA            25427..25503
                     /locus_tag="ACHOQQ_RS03800"
                     /old_locus_tag="pgaptmp_000760"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25461..25463,aa:Val,seq:gac)
     gene            25508..25584
                     /locus_tag="ACHOQQ_RS03805"
                     /old_locus_tag="pgaptmp_000761"
     tRNA            25508..25584
                     /locus_tag="ACHOQQ_RS03805"
                     /old_locus_tag="pgaptmp_000761"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25542..25544,aa:Val,seq:gac)
     gene            25692..25997
                     /gene="ydhR"
                     /locus_tag="ACHOQQ_RS03810"
                     /old_locus_tag="pgaptmp_000762"
     CDS             25692..25997
                     /gene="ydhR"
                     /locus_tag="ACHOQQ_RS03810"
                     /old_locus_tag="pgaptmp_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGU4469057.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26123..27727
                     /gene="ydhS"
                     /locus_tag="ACHOQQ_RS03815"
                     /old_locus_tag="pgaptmp_000763"
     CDS             26123..27727
                     /gene="ydhS"
                     /locus_tag="ACHOQQ_RS03815"
                     /old_locus_tag="pgaptmp_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HGU4469058.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADVAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEAIRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(27739..28551)
                     /gene="ydhT"
                     /locus_tag="ACHOQQ_RS03820"
                     /old_locus_tag="pgaptmp_000764"
     CDS             complement(27739..28551)
                     /gene="ydhT"
                     /locus_tag="ACHOQQ_RS03820"
                     /old_locus_tag="pgaptmp_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGU4469059.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARIAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(28555..29340)
                     /gene="phsC"
                     /locus_tag="ACHOQQ_RS03825"
                     /old_locus_tag="pgaptmp_000765"
     CDS             complement(28555..29340)
                     /gene="phsC"
                     /locus_tag="ACHOQQ_RS03825"
                     /old_locus_tag="pgaptmp_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGU4469060.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29337..30005)
                     /gene="ydhX"
                     /locus_tag="ACHOQQ_RS03830"
                     /old_locus_tag="pgaptmp_000766"
     CDS             complement(29337..30005)
                     /gene="ydhX"
                     /locus_tag="ACHOQQ_RS03830"
                     /old_locus_tag="pgaptmp_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4469061.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30069..30707)
                     /gene="ydhW"
                     /locus_tag="ACHOQQ_RS03835"
                     /old_locus_tag="pgaptmp_000767"
     CDS             complement(30069..30707)
                     /gene="ydhW"
                     /locus_tag="ACHOQQ_RS03835"
                     /old_locus_tag="pgaptmp_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGU4469062.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30720..32822)
                     /gene="ydhV"
                     /locus_tag="ACHOQQ_RS03840"
                     /old_locus_tag="pgaptmp_000768"
     CDS             complement(30720..32822)
                     /gene="ydhV"
                     /locus_tag="ACHOQQ_RS03840"
                     /old_locus_tag="pgaptmp_000768"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGU4469063.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(32843..33469)
                     /gene="ydhY"
                     /locus_tag="ACHOQQ_RS03845"
                     /old_locus_tag="pgaptmp_000769"
     CDS             complement(32843..33469)
                     /gene="ydhY"
                     /locus_tag="ACHOQQ_RS03845"
                     /old_locus_tag="pgaptmp_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGU4469064.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLTGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8061 a         8870 c         8291 g         8418 t
ORIGIN      
        1 cgtaaaggaa tctactttgt gaagtttatg ctcaatgcaa caggattgcc cttacaagat
       61 ctggtgttcg gtgcgtccgt ctactttcct ccgtttttca aagcattcgc gtttggattc
      121 gtcatctggc ttgtcgtaca ccgcctgctt cgtggctgga tctacgccgg tgacatctgg
      181 catcccttgt taatggattt atcgctgttt gcgatttgcg tttgccttgc tctggcaata
      241 ctgattgcgt ggtaactatg tcaattaaaa caattaagta tttctcaaca atcattgtag
      301 cggtagttgc ggttcttgcc ggatggtggc tgtggaatta ttacatgcaa tctccgtgga
      361 cacgcgacgg gaaaatacgc gcagaacagg tttctatcac cccacaggtg tcgggacgta
      421 ttgttgagct gaatataaaa gacaaccagc tggttaacgc gggggatctt ctgctcacga
      481 tcgataaaac gccctttcag atcgccgagc tgaacgcaca ggcacagctg gcgaaagcgc
      541 aatctgacct cgcaaaagcc aataacgaag cgaatcgtcg tcgtcatctc tcacaaaatt
      601 ttatttctgc cgaagaactc gataccgcta acctcaatgt taaagcgatg caggccagtg
      661 ttgatgccgc acaggcgacg ctgaaacaga cacaatggca actggcgcaa acggaaattc
      721 gcgccccggt gagtggatgg gtgactaacc tcactacccg catcggcgat tacgctgaca
      781 ccgggaaacc gctgtttgcc cttgtcgaca gccactcgtt ttacgtcatt ggttattttg
      841 aagaaaccaa gttgcgccat atccgcgaag gtgcaccagc acaaattacc ctctatagtg
      901 ataacaaaac gttacagggt cacgttttca gtatcggtcg ggcgatttat gatcagagcg
      961 ttgaaagtga ctcaagcctt attccggatg tgaaacctaa cgtcccctgg gttcgtctcg
     1021 cccaacgcgt tcccgtccgc tttgcgctgg ataaagttcc aggcgatgtc acgctggtgt
     1081 cgggcaccac ctgtagcatt gccgtaggtc aataatgaac gcatcgtcat ggtccttgcg
     1141 caatttgccc tggttcaggg ccacgctggc gcaatggcgt tatgcgttac gcaataccat
     1201 tgctatgtgt ctggcgctga cggttgccta ttatttgaat ctggatgaac cctattgggc
     1261 gatgacctcg gctgcagtgg ttagctttcc caccgttggc ggtgtcatca gcaaaagcct
     1321 cggacgcatc gctggcagtt tactgggagc cattgcggca ctgcttcttg ccggacatac
     1381 gctcaatgag ccgtggtttt ttctattgag catgtcggcg tggcttggct tttgtacctg
     1441 ggcctgtgcg cacttcacga ataacgtcgc gtatgcattt caactggcgg gctacacggc
     1501 tgccatcatc gcctttccga tggttaatat tactgaggcc agccagctgt gggatatcgc
     1561 tcaggcgcgc gtttgcgagg tgattgtcgg tattttgtgc ggcggcatga tgatgatgat
     1621 cctgccgagc agttccgatg ctactgccct tttaaccgca ttgaaaaaca tgcacgcccg
     1681 attactggaa catgccagtt tactctggca gcctgaaaca accgatgcca ttcgtgcagc
     1741 acatgaaggg gtgattgggc agatactgac catgaatttg ctgcgtatcc aggctttctg
     1801 gagccactat cgttttcgcc agcaaaacgc gcgccttaat gcgctgctcc accagcaatt
     1861 acgtatgacc agtgtcatct ccagcctgcg acgtatgttg ctcaactggc cctcaccgcc
     1921 aggtgccaca cgagaaattc tcgaacagtt gctgacggcg ctcgccagtt cgcaaacaga
     1981 tgtttacacc gtcgcacgta ttatcgcccc gctacgcccg accaacgtcg ccgactatcg
     2041 gcacgtcgcc ttctggcagc gactacgtta tttttgccgc ctttatctgc aaagtagtca
     2101 ggaattacat cgtctgcaaa gcggtgtaga tgatcatacc agactcccac ggacatccgg
     2161 cctggctcgt cataccgata acgccgaagc tatgtggagc gggctgcgta cattttgtac
     2221 gttgatgatg attggcgcat ggagtattgc ttctcaatgg gatgccggtg ccaatgcatt
     2281 aacgctggca gcaattagct gcgtactcta ctccgccgtc gcagcaccgt ttaagtcgtt
     2341 gtcactgctg atgcgcacgc tggtgttact ttctctattc agctttgtgg tcaaatttgg
     2401 cctgatggtc cagattagtg atctgtggca atttttgctg tttctctttc cactgctggc
     2461 gacgatgcag cttcttaaat tgcagatgcc aaaatttgcc gcactgtggg ggcaactgat
     2521 tgtttttatg ggttcgttta tcgctgtcac taatcccccg gtgtatgatt ttgctgattt
     2581 tcttaacgat aatctggcga aaatcgttgg cgtcgcgttg gcgtggttag cgttcgccat
     2641 tctgcgtcca ggatcggatg ctcgtaaaag ccgtcgccat attcgcgcgc tgcgtcggga
     2701 ttttgtcgat caactaagcc gccatccaac actgagtgaa agcgaatttg aatcgctcac
     2761 ttatcatcac gtcagtcagt tgagtaacag ccaggatgcg ctggctcgcc gttggttatt
     2821 acgctggggt gtagtgctgc tgaactgttc tcatgttgtc tggcaattac gcgactggga
     2881 atcgcgttcc gatccgttat cgcgagtacg ggataactgt atttcactgt tgcggggagt
     2941 gatgagtgag cgtggcgttc agcaaaaatc actggcggcc acacttgaag aattacagcg
     3001 gatttgcgac agccttgccc gtcatcatca acctgccgcc cgtgagctgg cggcaattgt
     3061 ctggcggctg tactgctcgc tttcgcaact tgagcaagca ccaccgcaag gtacgctggc
     3121 ctcttaatta cttaattaca ccacaggcat agcgttcacc gccaccgccc agcggtttag
     3181 gttgatcgga catattatcg ccgccaacgt ggaccatcag cgctttgtct ttgatttcat
     3241 ccagtgattt cagacgaggc gcgatgacgg catcggtagc tttgccgtca ttattgacta
     3301 ccagtgcagg cagatcgcct aaatgcccgg caccttctgg cccttcatgt ttgccggtat
     3361 tttgtggatc aagatgcccg cctgcggatt ccgcggcgct ggctttgcca tctttggtgg
     3421 ctggctggca gcttcctttg gcatgaatat ggaagccatg ttcaccgggg ggtaatgctt
     3481 tcagatcggg cgaaaactcc agacctttat cggtttcagt aatggtgacg ctaccaattg
     3541 actgcccaac cccttgcgac gtgacgaggt tcatctcgac tttttcactg gcagcttgtg
     3601 cgccggttgc aacaaccagc gccagaatag ccagactaaa acgtttcata ggacctccgt
     3661 tcattgtgca ttcctgaata agtgtaaacc agtgacacat ttttgaacgg cggctattcc
     3721 taaaagtgct tacggtacgt cgtaccccag tgccgcttta cggatacgaa accattgttg
     3781 acgggtcatt ttcagtgttt ctgcttcgac agctgcccgt acgcgctcaa ttttacctga
     3841 gccgataatt ggcagcggct gcgacggtaa acgtaatacc caggcgtaaa ccacctgttc
     3901 aatcgagccc gcgtttaact cctccgccac cacagccagt tcatcacgta gcggctggaa
     3961 ataatcatca ttaaacagac gaccaccacc aaggcaggac cacgccatcg gacgaacacg
     4021 cagttgttgc agttggtcga gcgtgccatc cagcagtaac ggctgatgta ccggggatat
     4081 ttccacctga ttagtggcaa gggtaaacgg caggcgtgat tgcaacaggg caaattgcgc
     4141 gggcgtgaag ttggatacgc caaaatgacg cactttgccg ctctgatgca gatgtttgaa
     4201 cgcgtccgcc acttcatcgg catccattaa cgggtccggg cggtggatta acagcagatc
     4261 cagatgatcg gtcgcgagat taatcagcga ctgttcggcg cttttaataa tatggtcgcg
     4321 gtcagtgatg taatggccaa tgacgttttc ttcacgcgca gtcgtagcaa tgccacattt
     4381 gctgacgatt tccatccgtt cacgcagatg tggggccagt ttcagcgcct cgccaaacgc
     4441 cgcttcgcac tgatagccac cataaatatc agcatggtcc acggtggtca cgccgagatc
     4501 cagatgctct tcaataaaac tgaccagctg gcgggcggac atattccagt ccatcaatcg
     4561 ccagtagccc atcacaaaac gggaaaactc cgggccttgc ggcgcaatag taatacgctg
     4621 aaccatattc gcttcctctt atcagatatg agaggagtat acgcaagatt aggctcaaaa
     4681 gagtgatggt tgctccggtt cgtctgatga cgctggctta tttgcacgta atttgcgcat
     4741 taatcgctgc cgacaaaggc gcagcacctc ttgtttttcg ccatcgctca ttttattcca
     4801 gttaaaacgc tcatcccggc ttcgaaaaca gccgcgacaa aacccgcgtt cgtcagactg
     4861 gcaaattccc cggcacgggc tctggacggg aaagaactct aattgctccg ccacttcgcc
     4921 ctcctcagat aagattatta ccattattga agctgttaat gtccaaagta gcaactttgc
     4981 ttgcgctaga ccgactggtc tactacactc caacacatga acaaacacac cgaacatgat
     5041 actcgtgaac atctcctggc gacgggcgag caactttgcc tgcaacgtgg attcaccggg
     5101 atggggctaa gcgaattact aaaaaccgct gaagtgccga aagggtcctt ctatcactac
     5161 tttcgctcta aagaagcgtt tggcgttgcc atgcttgagc gccattacgc cgcatatcac
     5221 cagcgactga cggagttgct gcaatccgga gaaggtaact accgcgaccg catactggct
     5281 tattaccagc aaacactgaa ccagttttgc caacatggaa ccatcagtgg ttgcctgaca
     5341 gtaaaactct ctgccgaagt gtgcgatctg tcagaagata tgcgcagcgc aatggataaa
     5401 ggtgctcgcg gcgtgatcgc cctgctctct caggcgctgg aaaatggtcg taatagccat
     5461 tgtttaacct tttgcggcga accgctacaa caggcgcaag tgctttacgc gctgtggctg
     5521 ggtgcgaatc tgcaggccaa aatttcgcgc agtttcgagc cacttgaaaa cgcgctggca
     5581 catgtaaaaa ccattattgc gacgtctgcc gtttagcagg cattttttta tcaccagacg
     5641 accgggagcc tttatgtcat ctgaaaaact gtattcccca ctgcaagtgg gcgcgatcac
     5701 ggcggcaaac cgtattttta tggcaccgct gacgcgtctg cgcagtattg aaccgggtga
     5761 catccctacc ccgttgatgg cggaatacta tcgccaacgt gccagtgctg gtttgattat
     5821 tagcgaagcc acgcaaattt ctgcccaggc aaaagggtat gctggtgcgc ctggcatcca
     5881 tagccaggag caaattgccg cgtggaaaaa aatcaccgct ggcgttcatg ctgaaaatgg
     5941 tcatattgcc gtgcaactgt ggcataccgg acgtatttct cacgccagcc tgcaacctga
     6001 cggtcaggca ccggtagcac cttcagcact tagcgcggga acacgcactt ctctgcgcga
     6061 tgaaaatggt caggcgatcc gtgttgaaac atccatgccg cgtgcgcttg aactggaaga
     6121 gattcctggt atcgtcaacg atttccgcca ggccatagcc aacgcccgcg aagccggttt
     6181 tgatctggtc gagctccact ctgctcacgg ttatttgctg catcagtttc tttcgccttc
     6241 ttcgaaccat cgtaccgatc agtacggcgg tagcgtggaa aatcgcgcac gtctggtgct
     6301 ggaagtggtc gatgcaggca ttgaagaatg gggtgccgat cgcattggca ttcgcgtttc
     6361 gccaatcggt actttccaga acacggataa tggcccaaat gaagaagccg atgcgctgta
     6421 cctgattgaa caactgggta aacgcggcat tgcttatctg catatgtcag aaccggattg
     6481 ggcgggaggt gaaccgtata ctgatgcgtt ccgcgaaaaa gtacgcaccc gtttccacgg
     6541 tccgattatc ggcgcaggtg catacacagt agaaaaagct gaaacgctga tcggcaaagg
     6601 gttaattgat gcggtggcat ttggtcgtga ctggattgcg aaccctgatc tggttacccg
     6661 cttgcagcgc aaagccggac ttaacccaca gcgtgctgaa agcttctatg gcggtggtgc
     6721 ggaaggctac actgattacc cgacgctgta atccaacatt gcgagcggcg caaagccgcc
     6781 gctatactaa aacaacattt tgaatctgtt agccattttg aggatataaa gatgcgtctt
     6841 cttcatacca tgctacgcgt tggcgatttg caacgctcca tcgattttta taccaaagtt
     6901 ctgggcatga aactgctgcg taccagcgaa aacccggaat acaaatactc actggcgttt
     6961 gttggctacg gcccggaaac cgaagaagcg gtgattgaac tgacctacaa ctggggcgtg
     7021 gataaatacg aactcggcac tgcttatggt cacatcgcgc ttagcgtaga taatgccgct
     7081 gaagcgtgcg aaaaaatccg tcaaaacggg ggtaacgtga cccgtgaagc gggtccggta
     7141 aaaggcggta ctacggttat cgcgtttgtg gaagatccgg acggttacaa aattgagtta
     7201 atcgaagaga aagacgccgg tcgcggtctg ggcaactaat ctcctgccgg gcgcgaactc
     7261 atcgcgcccg catctttact gcatcgacaa gtaatatttg tcataatgcg cgctgcaatt
     7321 tatccgtatt aagagaatca gatgtccgat aacgctcaac ttaccggtct gtgcgaccgt
     7381 tttcgtggtt tttatcctgt tgtgatcgat gttgaaacag ccggatttaa cgccaaaacc
     7441 gatgcgctgc ttgagattgc cgccatcacc ctgaaaatgg atgaacaagg ctggctgatg
     7501 ccggacacca cattacattt ccacgtcgaa ccatttgtcg gcgcaaattt gcaaccagaa
     7561 gcccttgcct tcaacggcat tgacccgaat gatcccgatc gcggcgcggt cagcgaatac
     7621 gaggcgctgc acgaaatttt taaagttgta cgtaaaggta ttaaagcgag cggctgtaac
     7681 cgcgccatta tggtggcgca caacgccaat ttcgatcaca gctttatgat ggccgccgct
     7741 gaacgcgcct cactgaaacg taacccgttc caccctttcg ccacttttga cactgctgca
     7801 ctggccgggc tggcactcgg acaaaccgta ttgtcaaagg cttgccagac cgctggcatg
     7861 gacttcgaca gcacccaagc tcactccgcg ctgtacgaca ccgaacgcac tgcggtgctg
     7921 ttttgtgaaa tcgtcaaccg ctggaaacgt ctgggaggct ggccgctacc tgccgccgaa
     7981 gaggtgtaat cgagtcgatg cctgatgaca tgcaatgatt caggcatcta tagtgaggct
     8041 attccacgca tcatgcatga tattcacggg gaatagcgtt aatggcagat aatccaaacc
     8101 tttcatcgcc cctgccggac gtgttttcac cggcgacccg cgactggttt cttagcgcct
     8161 ttaaacagcc gaccgctgtc cagtcgcaaa cctggcatgt ggcggcgcga ggcgaacatg
     8221 cgctggtgat tgcaccgacc ggctccggga aaacgctggc agcatttctc tacgccctcg
     8281 atcggctctt ccgcgaaggc ggcgaagata cccgcgaggc gcataagcgt aaaacctcac
     8341 gcattctcta tatttcaccg ataaaagccc tgggcaccga cgttcagcgc aacttgcaga
     8401 tcccgttgaa gggtattgcc gatgaacggc ggcggcgcgg cgaaacggaa gtcaatcttc
     8461 gcgtggggat ccgtactggc gatacgcctg cacaggaacg cagcaaactc acccgtaatc
     8521 caccggatat tctgatcacc acacccgaat cactctatct gatgctgacc tcccgcgcgc
     8581 gcgaaacgct acgcggcgtc gaaacggtaa ttattgatga agtccacgcg gtagcgggca
     8641 gtaaacgtgg tgcgcatctg gcgttaagtc tggagcggct cgatgcgctg ctccacacct
     8701 cagcacagcg aattggcctt tctgccactg tgcgctcagc cagcgatgtg gcagcatttc
     8761 ttggcggcga tcgcccggtt acggtagtca acccgcccgc aatgcgccat ccgcagatac
     8821 ggattgtcgt accggtcgcc aatatggatg atgtctcatc ggtcgccagc ggcactggcg
     8881 aagacagcca tgccggccgg gaaggctcca tctggccata tattgaaacg ggtatccttg
     8941 atgaagtgtt gcgccatcgc tcgaccattg tctttactaa ttcgcggggg ctggcggaaa
     9001 aactgacggc acgattaaat gagctttacg ccgcacgctt acagcgttcc ccttctatcg
     9061 ccgttgatgt ggcccatttc gagtcgacct ccggcgcaac ctctaaccgt gtgcaaagta
     9121 gcgacatttt tattgcccgc tcacaccacg gctccgtctc taaagaacaa cgagcaatca
     9181 ccgaacaggc gctgaaatcg ggtgaattac gctgcgtggt cgcaacctcc agtctcgaac
     9241 tggggattga tatgggcgct gtggatctgg tgattcaagt ggcaacgccg ctttctgttg
     9301 ccagtgggtt acaacgcatt ggtcgcgccg gacatcaggt tggcggtgta tctaaagggc
     9361 tgtttttccc ccgaacccgg cgtgacttag tcgattccgc agtcattgta gagtgtatgt
     9421 tcgcaggcag gctggaaaac ctgacaccac cgcataatcc actcgacgtc cttgcgcagc
     9481 acaccgttgc cgccgcggcg atggatgcat tacaggtaga cgaatggtac tcccgcgtac
     9541 gccgtgccac accgtggaaa gatctgccaa gacgtgtttt tgacgccacg ctggatatgc
     9601 tttccgggcg ctatccctct ggcgattttt ctgcttttcg accaaaactg gtctggaacc
     9661 gggagaccgg gatattgtcc gcccgacctg gcgctcagct gttggcggtt accagcggtg
     9721 gcaccattcc ggatcgtggc atgtatagcg tgttattacc cgaaggtgaa gaacaggccg
     9781 gttcgcggcg ggtgggtgaa ctggatgagg agatggtata tgagtcgcgg gtgaacgata
     9841 ttatcactct cggcgctacc tcatggcgga tccagcaaat cacccgcgat caggtgattg
     9901 tgactcctgc tccgggtcgt tctgcccggc ttcccttttg gcgtggtgaa ggtaacggac
     9961 gtccggctga attaggcgag atgatcggcg attttcttca tttactggcg gatggcgcgt
    10021 tcttttccgg gactattccc ccgtggctgg cagaagaaaa tacgatcgcc aatattcagg
    10081 ggttgattga cgagcagcgc aacgcgacgg gtattgttcc ggggagtcgt catctggtcc
    10141 tcgaacggtg ccgtgatgaa attggcgact ggcgtattat tttgcactct ccctatggaa
    10201 gacgggtgca tgaaccctgg gcgctggcga ttgccgggcg aatacatgcg ctatggggcg
    10261 ctgacgcgtc tgtggtcgcc agtgatgacg gcattgttgc gcgcattcct gacactgatg
    10321 gtaaattgcc cgacgccgcg atttttttgt ttgaaccaga aaagttgctg caaattgtcc
    10381 gcgaggcggt aggcagctca gcacttttcg ccgcccgttt tcgcgaatgc gccgcgcggg
    10441 cgttattaat gccgggccgc acgccgggcc atcgcacccc gctctggcaa caacggctgc
    10501 gcaccagtca gttgctggaa atcgctcagg gatatccgga ttttccggtc attctcgaaa
    10561 ccctacgcga atgtctgcaa gatgtttatg atcttcccgc actggaacgt ttgatgcgtc
    10621 gcctgaacgg tggcgaaatt caaatatccg atgtaacgac tactacgccc tcgcctttcg
    10681 ccaccagttt attgttcggc tatgtcgcgg aatttatgta ccagagcgac gccccgctgg
    10741 cagagcgccg ggcatccgta ctgtcactgg acagcgagtt actgcgcaat ctactcgggc
    10801 aggtcgatcc gggggaatta ctcgacccgc aggtcattcg ccaggtggaa gaagagttgc
    10861 aacgactggc tcctggcaga agagcgaaag gtgaagaagg attgttcgac ctgctgcgcg
    10921 aactggggcc aatgaccgtt gaagacctgg cgcaacggca tacaggcagc agtgaagaga
    10981 ttgcgtcgta tctggaaaat cttcttacag taaaacgcat cttcccggcg atgattagcg
    11041 gacaggagcg tcttgcctgt atggatgatg ccgccaggct gcgcgatgcc ctcggcgtac
    11101 aactaccaga gtctctgcca gagatttatt tgcatagagt cagttacccg cttcgcgacc
    11161 tctttctgcg ctatctccgg gctcatgctc tggtcacggc tgaacaactg gctcatgagt
    11221 ttagtctcgg tattgccatt gtcgaagagc agcttcagca actgcgcgaa cagggtctgg
    11281 tgatgaatct gcaacaagac atctgggtga gcgatgaagt atttcgtcgt ctgcgtttgc
    11341 gctcgctgca agccgccaga gaagcgacgc gtcccgttgc ggccacgacc tatgcgcgat
    11401 tgctgctgga acgtcagggc gtattacccg ccaccgatgg tagcccggcg ctctttgcct
    11461 caacatcgcc aggcgtttat gagggcgtgg atggcgtgat gcgggtgatc gaacagcttg
    11521 ccggagtcgg tttacccgcc tcactctggg aaagccagat cctgcctgcc cgcgtacgcg
    11581 actattcgcc agaaatgctc gatgaattac tggcaaccgg tgcggttatc tggtcggggc
    11641 aaaaaaaact gggtgaagat gacggcctgg tggcactgca tctacaggaa tatgctgcag
    11701 aatcgttcac tcccgctgaa gcggatcagg cgaatcgttc ggcgctgcaa caagcgatag
    11761 tcgctgttct ggctgacgga ggagcctggt ttgcacaaca aatcagccaa cggatacgcg
    11821 acaaaatcgg cgaatcggtt gatccctcgg cactgcaaga ggcgttatgg gcgctggtct
    11881 ggcaaggcgt catcaccagc gatatttggg caccgttacg cgccctcacc cgcagcagtt
    11941 ccaacgcacg cacctcaact cgccgcagtc accgggctcg tcgtgggcgt cctgtctatg
    12001 cacaacccgt ctcgccgcgg gtatcttaca acacaccaaa tctggctgga cgctggtcgt
    12061 tattgcaggt ggagccacta aacgataccg aaaggatgct ggcgctggcg gaaaatatgc
    12121 tcgaccgcta cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt
    12181 ttccatcgat gcaaacgctt tgtcgaagta tggaagattc cgggcgaatt atgcgaggtc
    12241 gttttgtaga aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgcttgc
    12301 gcgacctggc gacacaagcc gcgcaaacgc gccactatac accagtggcg ctctccgcca
    12361 acgatccggc taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg
    12421 taccaacgcg tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc
    12481 tggcgcaagg tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag
    12541 aggttttcca cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa
    12601 cagaagtgaa tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg
    12661 gattttcaag ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg
    12721 ttcgcatccg tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt
    12781 tagcggcagt ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga
    12841 tatcacaacc gccgaccagc tcgccgtcaa cccacagttg cgggaaggtc ggccagttag
    12901 catatttcgg cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac
    12961 gttcgccaca tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt
    13021 tcggtgaacc tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt
    13081 cgatagtggt gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg
    13141 tagcgggtca gtgcagcatc ggaaaataac attttcatca cgctttagct attttatccc
    13201 tttgctctat ttgttgcatt tcaaatattc gtttcttctc tgatgcgcgg caaactggcg
    13261 tatttataac catttttagc tttcactgct gcattttttt gcgctcgcca acgaaacgta
    13321 ttttttaaca ataaaagcta ttaactttct cttcttctat gcattagaat catcaagttt
    13381 tgtaaatcag acgcaggcat gatagacctg ccttaacaga gggacgctca gtggcgcgga
    13441 taaaccgtat ttcgatcacg ctctgtgctt tactttttac caccctgcct ttaacgccta
    13501 tggcccatgc ttcaaagcaa gccagggaga gttctgctac cactcatatc accaaaaaag
    13561 cagataaaaa gaaaagcacg gcaaccacca aaaagaccca gaaagccagc cagaaaacag
    13621 cgaaaaaagc cgccagtaaa agtacgacca aaagcaaaac cgcttcttcc gttaaaaaat
    13681 cttccattac cgcttctaaa aacgccaaaa ctcgcagcaa acacgccgtc aataaaacgg
    13741 cctcagccag ctttaccgaa aagtgtacca aacgtaaggg ctataaatcg cattgtgtga
    13801 aagtcaaaaa tgccgcgtca ggaactcttg ccgacgcgca caaagcgaag gtgcaaaaag
    13861 ctacgaaagt ggcaatgaat aaactgatgc agcaaattgg taagccatat cgttggggtg
    13921 gcagctcacc gcgtaccggt tttgattgca gcggcctggt ttattacgct tataaagatt
    13981 tggtaaaaat tcgtattccg cgtacggcga atgaaatgta tcacctgcgc gatgcagcgc
    14041 caatcgaacg tagtgaactg aaaaacggcg acctggtctt tttccgtact cagggacgcg
    14101 gtacagccga tcatgtcggt gtgtatgtcg gcaacggcaa atttattcag tcaccgcgta
    14161 cgggtcagga aattcaaatc acttctctca gtgaagacta ctggcagcgc cactatgttg
    14221 gcgctcgtcg ggtaatgacc ccaaaaacac ttcgctaaaa ctttaccctg ttgttacggc
    14281 aacagggtaa gttcatcttt tgtctcacct tttaatttgc taccctatcc atacgcacaa
    14341 taaggctatt gtacgtatgc aaattaataa taaaggagag tagcaatgtc attcgaatta
    14401 cctgcactac catatgctaa agatgctctg gcaccgcaca tttctgcgga aactatcgag
    14461 tatcactacg gcaagcatca tcagacttat gtcactaacc tgaacaacct gattaaaggt
    14521 accgcgtttg aaggtaaatc actggaagag attattcgca gctctgaagg tggcgtattc
    14581 aacaacgcag ctcaggtctg gaaccatact ttctactgga actgcctggc accgaacgcc
    14641 ggtggcgaac cgactggaaa agtcgctgaa gctatcgccg catcttttgg cagctttgcc
    14701 gatttcaaag ctcagtttac tgatgcagcg atcaaaaact ttggttctgg ctggacctgg
    14761 ctggtgaaaa acagcgatgg caaactggct atcgtttcaa cctctaacgc gggtactcca
    14821 ctgaccaccg atgcgactcc gctgctgacc gttgatgtct gggaacacgc atattacatc
    14881 gattatcgca atgcacgtcc tggctatctg gagcacttct gggcgctggt gaactgggaa
    14941 ttcgtagcga aaaatctcgc tgcataataa ctgatggcaa atgcagcatt gcctgatgcg
    15001 cttcgcttac catgccaacg cggatgaggc gttaacagcg cattcggcaa gataaatcgc
    15061 acgttgtcag caactgtaac gcagaaggtt atccttctgc gtttttgttt aattagctgt
    15121 tagcaacgca aactgtttca ggttgttttc tggctgacat aaacaccagc aataatgcca
    15181 gtcccgcgac aatcgccccc atcaccggca caaagctgta tcccagccca gcggaaatta
    15241 ccgcaccacc agcggctgct cccagcgcat ttccaagatt aaaggcacca atattgactg
    15301 atgaagacag acctggcgct tcactggcga cacgcatcac gcgcatctgt aacggcggta
    15361 cgaccgcaaa ggttgcagcg ccccacacca ccatgctaat agctgcgccg aactcattgc
    15421 gggccaggaa cgggattgcc agcataatca ccatcagcag caacaaaaag cctttcaacg
    15481 tgccgttaac tgaacgatct gccagtttgc cgccgagata gttaccgata gagaatccga
    15541 caccaatcag caccagcatt gccgtgacga acaccggtgt tgcgtgggta atactttgca
    15601 gtaccggaga gatataggtg tagagagtaa acattgcacc agctcccagt accgtcgtca
    15661 gcaatgcaga cagcacctgc ggacgcatta ataccgccag ctcttttttc acttcaggtc
    15721 gtgcccctgc accaccttta ggtaatgaga agaacagact taccattgaa atcactccca
    15781 gccccgccgt tgccagaaat gacatccgcc agccgatggt ttcacccaac caggtcgccg
    15841 ccggcacgcc accgatattt gccagggtta accccataaa catagtggca actgcgctgg
    15901 cctgtttatg ttttggcacc acgcttgcag ccacgactga acccaaacca aaaaatgctc
    15961 catgattcag gctggtcaaa atgcgtgaaa gcatcagggt catataatcc ggcgcgatgg
    16021 cggaaagtac gttgccgagc gtgaaaattg ccatcaggaa aatcaacgca ctgcggcggg
    16081 cacgatgaga aagtagaagc gtcatcagcg gcgcgccaac cattacgcca actgcatagg
    16141 cactgattaa cattccggca gcgggaatcg agacatccac accgcgcgca atgacgggca
    16201 acaagcccat tggcgagaac tccgttgtcc cgataccaaa cgcgccaatc gccagcgcca
    16261 gcaacggata gttaattttc atgccttatc tccacctctt cgcgtcatta cgcgatattc
    16321 attaaagtgg cgaaagcatg acagcaatca caaaaaaatg aaaataacaa aaagagaaaa
    16381 cacttttgcc attttgctaa caaacaggaa ggagatgcga gggagaacgc gctccctcga
    16441 gaggaaatca gtgcagcgcg gcagtcaaac ccacggctac gatcaaaccg aggacgataa
    16501 tcgttgttac cagtgaaaat ttaaggtcgg tgctcatcaa gttttctcct tttttattac
    16561 cacacaaaaa gtgatattac gcatttttac acactgtgat gaaaaaatct cccgtcattt
    16621 ataatgataa gtgtttttac cacttcccct tttcgtcaag atcggccaaa attccacgct
    16681 tacactattt gcgtactggc cattgacccc ttcctgacgc tccgtgtcgt ttttccggcg
    16741 taccgcaaca cttttgttgt gcgtaaggtg tgtaaaggca aacgtttacc ttgcgatttt
    16801 gcaggagctg aagttagggt ctggagtgaa atggaatggc aacaataaaa gatgtagcga
    16861 aacgagcaaa cgtttccact acaactgtgt cacacgtgat caacaaaaca cgtttcgtcg
    16921 ctgaagaaac gcgcaacgcc gtgtgggcag cgattaaaga attacactac tcccctagcg
    16981 cggtggcgcg tagcctgaag gttaaccaca ccaagtctat cggtttgctg gcgaccagca
    17041 gcgaagcggc ctattttgcc gagatcattg aagcagttga aaaaaattgc ttccagaaag
    17101 gttacaccct gattctgggc aatgcgtgga ataatcttga gaaacagcgg gcttatctgt
    17161 cgatgatggc gcaaaaacgc gtcgatggtc tgctggtaat gtgttctgag tacccagagc
    17221 cgttgctggc gatgctggaa gagtatcgcc atatcccaat ggtggtgatg gactggggtg
    17281 aagcaaaagc tgacttcacc gatgcggtca ttgataacgc gttcgaaggc ggctacatgg
    17341 ccgggcgtta tctgatagaa cgcggccatc gcgaaatcgg cgttatcccc ggcccgctgg
    17401 aacgtaacac cggcgcaggc cgccttgccg gttttatgaa ggcgatggaa gaagcgatga
    17461 tcaaggtgcc ggaaagctgg attgtgcagg gtgattttga acctgaatcc ggttatcgcg
    17521 ccatgcagca aatcctgtcg cagccgcatc gccctactgc cgtcttctgt ggtggcgata
    17581 tcatggcaat gggcgcactt tgtgctgctg atgaaatggg gctgcgcgtc ccgcaggatg
    17641 tttcgctgat cggttatgat aacgtgcgca acgcccgcta ttttacgccg gcgctgacca
    17701 cgatccatca gccaaaagac tcgctgggtg aaacagcgtt caacatgctg ttggatcgta
    17761 tcgtcaacaa acgtgaagaa ccgcagtcca ttgaagtgca tccgcgcttg attgaacgcc
    17821 gctccgtggc tgacggcccg ttccgcgact atcgtcgtta atcacccgtt gcgggagtct
    17881 cttccggctc ccgcagccac tccttattca gcgtctcact atcgccgaga tactcaagca
    17941 accaggttaa cgcaggcgac atatcatttt gctgccatgt cagacaacat gccgaatccg
    18001 gaaaggggtt ttccagttct aatgctaccc acttcccctc attaagccac ggtttggcga
    18061 aatgtgttgg caccatccct atgcataatc ctgccgagat acaggttgcc gatgattccc
    18121 agtcaggcac gacaagtctt ttttggttat ccagcaacca ggtaatacgc ttaggtagcg
    18181 ttcgcgaggt gtcttcgcgc accaacgacg gccagttgcg caacgtatca tcgctgaacg
    18241 ggccatccat caacgccagc gggtgatggc tggcaacaac gcaactccag cttagcatcc
    18301 ccatatcccg gaaggcataa cgaccgccta ccggaatcgc gcgtgttgcg ccaatcgcca
    18361 gttccacgcg cccgtcggaa agcgcatccc agacaccgtt gaacacttcc tgaaagacaa
    18421 gaagttcgac atcatcaaaa tggcgataaa aatcaacgat catctgccgt gtacgttctg
    18481 gcctgacaat attatccact gcaatagcta actgaccacg ccagccgttc gctatctgct
    18541 gacattgctg gcgggtgatc tgcatttttt tgacaacaga gcgaccttct ttgagaaacc
    18601 acgccccggc agcggtcagt tccacatcac ggtgacgtcg ttcaaacagc ggcaccgcca
    18661 gccactcttc cagctgacgt acggtatagc tgaccgcaga aggaacgcga tgcaactcct
    18721 gggctgcggc actaaaacta ccattacgcg ctaccgcatc aacaacttcg agtgaatatt
    18781 ctgaccacat agtctgcctg caaaattttt gaaaccagtc atcaaatatt accgtttcac
    18841 aacactaatt tcactcccta cactttgcgg cggtgtttaa ttgagagatt tagagaatat
    18901 acatgcaacc tgggaaaaga tttttagtct ggctggcggg tttgagcgta ctcggttttc
    18961 tggcaaccga tatgtacctg cctgctttcg ccgccataca ggccgatctg caaacgcctg
    19021 cgtctgctgt tagtgccagc cttagtctgt tccttgccgg ttttgccgca gcccagcttc
    19081 tgtgggggcc gctttccgac cgttatggtc gtaaaccggt attattaatc ggcctgacaa
    19141 tttttgcgtt aggtagtctg gggatgctgt gggtagaaaa cgccgctatg ctgctggtat
    19201 tgcgttttgt acaagctgtg ggtgtctgcg ccgcggcggt tatctggcaa gcgttagtga
    19261 cggattatta tccttcacag aaagttaacc gaatttttgc gaccatcatg ccgctggtgg
    19321 gtctatctcc ggccctggct cctctgttag gaagctggct gctggtccat ttttcctggc
    19381 aggcgatttt cgccaccctt tttgccatta ccgtggtgct gattctgcct attttctggc
    19441 tcaaacccac gacgaaggcc cgtaacaata gtcaggatgg tctgaccttt accgacctgc
    19501 tacgttctaa aacctatcgc ggcaacgtgc tgatatacgc ggcctgttca gccagttttt
    19561 ttgcatggct gaccggttca ccgttcatcc ttagtgaaat gggctacagc ccggcagtta
    19621 ttggtttaag ttatgtcccg caaactatcg cgtttctgat tggtggttat ggctgtcgcg
    19681 ccgcgctgca gaaatggcaa ggcaagcagt tattaccgtg gttgctggtg ctgtttgctg
    19741 tcagcgtcat tgcgacctgg gctgcgggct tcattagcca tgtgtcgctg gtcgaaatcc
    19801 tgatcccatt ctgtgtgatg gcgattgcca atggcgcgat ctaccctatt gttgtcgccc
    19861 aggcgctgcg tcccttccca cacgcaactg gtcgcgccgc agcgttgcag aacactctac
    19921 aactgggtct gtgcttcctc gcaagtctgg tagtttcctg gctgatcagt atcagcacgc
    19981 cattgctcac caccaccagc gtgatgttat caacagtagt gctggtcgcg ctgggttaca
    20041 tgatgcaacg ttgtgaagaa gttggctgcc agaatcatgg caatgccgaa gtcgctcata
    20101 gcgaatcaca ctgatctata tcgatatact tatacttagg ctgctaacaa aattttgttg
    20161 tatgattgaa attagcggcc tatactaatt tcgagttgtt aaagctacga taaatattat
    20221 gtttttacgg ggacaggatc gttccctact cactatggat ggtcatttcg gcaagggttc
    20281 ctcctttccc tctgttctac gtcggattat agactcgcgg ttttttctgc gagatttctc
    20341 acaaagccca aaaagcgtct acgctgtttt aaggttctga tcaccgacca gtgatggaga
    20401 aactatgagt tcatcgtgta tagaagaagt cagtgtaccg gatgacaact ggtaccgtat
    20461 cgccaacgaa ttacttagcc gtgccggtat agccattaac ggttctgccc cggcggatat
    20521 tcgtgtgaaa aaccccgatt tttttaaacg cgttctgcaa gaaggctctt tggggttagg
    20581 cgaaagttat atggatggct ggtgggaatg tgaccgactg gatatgtttt ttagcaaagt
    20641 cttacgcgca ggtctcgaga accaactccc ccatcatttc aaagacacgc tgcgtattgc
    20701 cggcgctcgt ctcttcaatc tgcagagtaa aaaacgtgcc tggatagtcg gcaaagagca
    20761 ttatgatttg ggtaatgact tgttcagccg catgcttgat cccttcatgc aatattcctg
    20821 cgcttactgg aaagatgccg ataatctgga atctgcccag caggcgaagc tcaaaatgat
    20881 ttgtgaaaaa ttgcagttaa aaccagggat gcgcgtactg gatattggct gcggctgggg
    20941 cggactggcg cactacatgg cgtctaatta tgacgtaagc gtggttggcg tcaccatttc
    21001 tgccgaacag caaaaaatgg ctcaggaacg ctgtgaaggc ctggatgtca ccattttgct
    21061 gcaagattat cgtgacctga acgaccagtt tgatcgtatt gtttctgtgg ggatgttcga
    21121 gcacgtcgga ccgaaaaatt acgataccta ttttgcggtg gtggatcgta atttgaaacc
    21181 ggaaggtata ttcctgctcc atactatcgg ttcgaaaaaa accgatctga atgttgatcc
    21241 ctggattaac aaatatattt ttccgaacgg ttgcctgccc tctgtacgcc agattgctca
    21301 atccagcgaa ccccactttg tgatggaaga ctggcataac ttcggtgctg attacgatac
    21361 tacgttgatg gcgtggtatg aacgattcct cgctgcatgg ccagaaattg cggataacta
    21421 cagtgaacgc tttaaacgaa tgtttaccta ttatctgaat gcctgcgcag gtgctttccg
    21481 cgcccgtgat attcagctct ggcaggtggt gttctcacgc ggtgttgaaa acggccttcg
    21541 agtcgctcgc taaaggctat tctatcgccc ccgctccggg ggcgatttca gatcaggctt
    21601 ctgtacctgg ttgattcatg gcattttctc gtgccgccag cacacgttct accgtatcta
    21661 ccactgcctg agtttgtgga tcgatttcaa tgttgacgcg tgcgccaagt tttttcttcc
    21721 caagagtcgt gcgttccagt gtttccggaa ttaaatggac gcaaaaacgc gttggcgtga
    21781 cttcgccgac ggtcaggcta ataccgtcga tgccaataaa tcctttgtac aggatatatt
    21841 tcatcaactg actatcctgg actttaaacc agatctggcg attattttcc gaggttaata
    21901 ttttcgccac ttcagcggtg gtcataatat gacctgacat taagtgtccg ccaatttcat
    21961 cactgaattt cgccgcacgc tcaacgttta cccaatcccc cacttttaaa tcgccaagat
    22021 tggtaatgcg taacgtttct ttcatcaggt caaaactgac atggttgccg ttaatttccg
    22081 tcacggtcag gcagcaaccg ttatgcgcca cggacgcacc ggtttccagg ccgtccagca
    22141 tgtggtcggg taactccacc acatgcgtac gaaaatttgg tttctcgtca atcgacacca
    22201 gttttgcggt gccctgtaca atccccgtaa acatacttac aactcctgaa atcagttaag
    22261 acattctgtt cagcacaata gcaggtggca aacgccctta ccagtgaagg ggtaagaatg
    22321 actatttttt cactggagaa ttaataaatc ctcgctacaa tagactgaat ttcccctgct
    22381 tcttcttttt gctgcccatt caggcggctt tttagtctct catataacta caaataaaag
    22441 gtgttcacgt gcagaagtat atcagtgaag cgcgtctgtt attagcatta gcaatcccgg
    22501 tgattctcgc gcaaatcgcc caaactgcga tgggttttgt cgataccgtg atggcgggcg
    22561 gctacagtgc caccgacatg gcggcggtcg ctatcggtac ttctatctgg cttccggcga
    22621 tcctctttgg tcacggactg ctgctggcat taacgccggt tatcgcgcaa ttaaatggtt
    22681 ccggtcgacg tgagcgcatt gcgcatcagg tgcgacaagg tttctggctg gcaggttttg
    22741 tttccgttct cattatgctg gtgctgtgga atgcaggtta cattatccgc tccatggaaa
    22801 acatcgatcc ggctctggcg gacaaagccg tgggttatct gcgtgcgttg ttgtggggcg
    22861 cgccgggata tctgttcttc caggttgccc gtaaccagtg tgaaggtctg gcaaaaacca
    22921 agccgggtat ggtaatgggc tttatcggcc tgctggtgaa catcccggtg aactatatct
    22981 ttatttatgg tcatttcggt atgcctgagc tcggtggcgt tggttgtggc gtggctactg
    23041 cggcggtgta ttgggtcatg ttccttgcca tggtttctta cattaaacgc gcccgctcca
    23101 tgcgcgatat tcgtaacgaa aaaggcaccg cgaaacccga tcctgcggtt atgaaacgac
    23161 tgattcaact cggtttgccg attgcgctgg cactattctt tgaagtgaca ctgtttgccg
    23221 tcgtggctct gttagtgtct ccgctcggta ttgttgatgt cgcaggacac cagattgccc
    23281 tgaactttag ttcactaatg ttcgtgcttc caatgtcgct ggcggcagcg gtaactatcc
    23341 gcgtaggtta tcgtctgggt cagggctcaa cgctggatgc gcaaaccgct gcgcggaccg
    23401 ggcttatggt gggtgtctgt atggcaaccc tgacggccat attcacggtt tcactgcggg
    23461 agcaaatcgc cctgttgtac aacgacaatc ccgaggttgt aacgctggct gcgcatttga
    23521 tgttgctggc ggcggtatat cagatttctg actcaatcca ggtgattggc agtgggattt
    23581 tgcgtggtta taaagatacg cgttccattt tctatatcac ctttacggct tactgggtgc
    23641 tgggcttgcc aagcggctat attctggcac tgaccgatct ggtcgttgaa cctatggggc
    23701 cagcaggctt ctggataggc tttattattg gcctgacgtc ggcagccatt atgatgatgc
    23761 tgcgtatgcg gttcctgcaa cgtatgccgt cagccatcat tctgcaacga gcgtcccgct
    23821 aataaagaca aggcgcaacc ttcacgggtt gcgcctgtat ttttacgcag gctggagcgt
    23881 tgcgccaatc ccgtcttcgt ctggctgtag tttcagtgca ttacacagag tttgccgact
    23941 ggcatctgtt atctcaacgc gtatatcccc ggtaacttcc cctttcacat tgatgaagtc
    24001 aggtgaaatt tctttcgcac tggtttgcag gcaaatcgct ccctctccgc gcaggcgttt
    24061 cactttcagt tgcacatgcg ttgccgcacc agtgatcagc cacagttttc cgccattcag
    24121 attgatgtta ccaatactga aagtgacact gtcacctttt accgtactgc cgtcgagaat
    24181 caatacaccg ccctgctgta cagtaatcgt tgattgttgc agatctgcgc catagccaat
    24241 aatcattgaa gcgccgtcca gtaatgcaac actcccggtc agttcaaccg gtgcgcgatc
    24301 ttcctctacg acaaaatttt cgtccgcgct ggtgggtgac aacaggatca ctgttccacc
    24361 ttgtattacc gcattttcca gcgtaccggc atgcacttca gcccgaccac ccacattaac
    24421 ggacaggttt tgcgtcttac cggaactgta gagctgaaga ccatctgtcc ccagacgata
    24481 gatggctcca ttctcaataa tagtgtcaga gtcttcacca ccgagattat tcatggtgcc
    24541 accagcaagc aacgtatcac tggctttccc cgccagcata aaaacgccgc cctgattaat
    24601 ctcacaattg ctggcttccc cacctggata caccaacaat tcaccacctt catctaccac
    24661 gaccgctgag gttgtcccat tgaccaacag gccgccttct tgatcgagaa taattttttc
    24721 cgcgcgatgt ccttccagta cacgcaggtt accgccattt tccagcaaca aaccgcaggc
    24781 ataaccttta tcaacgctga attcaccttc gggatggcgg ccattaaccg tagcgagcgt
    24841 agagagacta attgcgcctc cctcggactg aacaatatct gtggcaatgg caccgtcgct
    24901 gaccgatagc gtgccattgt taccgtactg tgtgcatttg gcgatcccac cggttgccac
    24961 tttcaaccag ccagcccccg tgaccacggt attttcggct cttccgccag cttcaacaat
    25021 caactcgcct tcagtaagtt gcgtcgcgcc aatgacctcg ccggtcttaa caatttgcac
    25081 attcccgtcg ctcatcaagt ttttcgcatc agatcccatg atttattcct ttgctgcatc
    25141 tgtgtgcctt tattgctacc taagtgtaaa ggctacggag gatttatcca cgacagattt
    25201 gagatggtgg caaacaactc tgtttaaact ctgatacacg aattattggg ttgtatcaga
    25261 tgtaaatgcg atcctgaata aaaatcgccc ttgcaaatca acaaaatata gccaatttgc
    25321 ttaaaagctc tgcaaacggt cgaaatagcg cagaaaatta cgttttgcct cttgccacct
    25381 tcccactctg ccgctaatat tcgtccccgt tgtcacctac aacgttgcgt tcatagctca
    25441 gttggttaga gcaccacctt gacatggtgg gggtcgttgg ttcgagtcca attgaacgca
    25501 ccatcctgcg tccgtagctc agttggttag agcaccacct tgacatggtg ggggtcggtg
    25561 gttcgagtcc actcggacgc accagatttt cttaatctgg tcttctcctt tttccctctg
    25621 cttcttctct gtatccaata cgttaaaaga tttacactgt cttcgtatgc gttatcagaa
    25681 ggagaatcgc tatggcaact ttgttacaac ttcatttttc tttcaatggc ccatttggcg
    25741 acgcaatggc tgagcagctt aaaccgcttg ctgagtcgat taatcaggaa cctggttttc
    25801 tgtggaaggt atggaccgaa agtgaaaaga accacgaagc cggtgggatc taccttttca
    25861 ctgatgaaaa aagcgccctt gcctatctgg aaaaacacac tgcccgactg aaaaatctcg
    25921 gcgttgagga agttgtcgcc aaagttttcg atgtcaatga gccactgagt caaatcaatc
    25981 aggcaaaact cgcctgacag attttaatca agggcggtta gcgccctttt catccctgtc
    26041 tgaaatttct caaattctaa aaatttcaac caaacttatc tgataacact aaattcgaaa
    26101 gaatgcgtac aggtaagtaa caatgaaaaa aattgctatt gtgggtgccg ggcctacggg
    26161 tatctacacc ttattctcgc ttctacagca acaaactcca ctttctattt ctatcttcga
    26221 gcaggctgat gtggccggtg tcgggatgcc atacagtgat gaggaaaact caaaaatgat
    26281 gctggcaaat attgccagta ttgaaatacc gccgatttat tgtacgtatc tcgaatggct
    26341 acaaaagcaa gaagccagcc atctccagcg ttatggcgtt aaaaaagaaa ccttgcacga
    26401 tcgtcagttt ttaccgcgaa ttctgctggg cgaatatttc cgcgatcaat ttttacgatt
    26461 agtagaccag gcacgaaagc aaaaatttgc agtggctgtt tatgaatcat gccaggttac
    26521 cgatctgcaa attacaaatg ctggcgtcat gctcgctaca aatcaggatt tacccagcga
    26581 gacgtttgat ttagcggtga ttgccacggg tcacgtctgg cctgatgaag aagaagcaat
    26641 ccgaacgtat tttcccagcc cgtggtcagg cctgatggaa gcaaaggtcg atgcgtgtaa
    26701 cgtgggtatt atgggaacat ccttgagcgg actggatgcg gcaatggcag tggcaattca
    26761 gcatggttcg ttcattgaag atgataaaca acacgtcgtt tttcaccgcg ataacgcaag
    26821 tgaaaagcta aatattacgt taatgtcgcg cacgggtatt ttacccgaag ccgatttcta
    26881 ttgccctatt ccctacgagc ccttacacat cgtcaccgat caggcattaa atgctgagat
    26941 tcaaaaaggc gaatatggcc ttttggatcg ggtatttaga ttgatagtag aggaaatcaa
    27001 gtttgctgat ccagactgga gtcaacgcat agccttagag agcctgaatg tcgattcctt
    27061 tgctcaagcc tggtttgccg agcgcaaaca acgcgaccca tttgactggg cagaaaaaaa
    27121 tctccaggaa gtcgaacgca ataaacgaga aaaacatact gttccctggc gttatgtcat
    27181 tctgcgtctg catgaagccg tacaggaaat tgttccacat ctgaatgaac acgaccataa
    27241 acggttcagt aaaggccttg cccgggtttt catcgataat tatgcggcaa tcccttcaga
    27301 gtctattcgt cgcctacttg ccttacgtga agcgggaatc attcatattc tcgctctcgg
    27361 tgaagactac aaaatggaaa ttaacgagtc gcgcaccgtc ctgaaaacgg aagacaacag
    27421 ctactcgttt gacgttttta ttgatgcccg cgggcagcgt ccgcttaaag tgaaagatat
    27481 tcctttccct ggactacgcg aacaattaca gaaaacaggg gatgaaatcc ctgatgttgg
    27541 tgaagattat acgttacagc aacccgaaga tattcgtggg cgcgtagcgt tcggcgcgtt
    27601 gccctggttg atgcacgacc agcctttcgt tcagggactt acggcatgtg cagaaattgg
    27661 tgaggcgatg gctcgggcgg tcgtaaaacc tgcctcccgt gcacgtaggc gtctttcgtt
    27721 tgattaaagg taaagctatc agcactcaac aaactgatgc tgttcattaa gacgatagcg
    27781 gactccggct cgaatattgt tttcaccttc aatggcgacg gcgaaacgaa ctctttcgcc
    27841 gtcatgatat gccagtgcgg cgcttcctcc tggcccgaga ataattgagt cgaccacgcc
    27901 cgtgctcact accgttgaat tttcaccgct ggcaatgatg tgtacattgt cgccgctgtt
    27961 ggcaattctg gcatttgcgc caaagctggc gatttgcacc aggtcaccat tactagcgac
    28021 atgacatcgt tcacccaatg tacaaacgcg tactcgcatt ccggtattcg caatccgact
    28081 gctatctcca gcgctgctta tccgcgaaga attgccggtt acagcaatcc gtgcgcgctc
    28141 accggagctg ccaatgtgac tgttaaagcc gacacttcca atccgcacgc tataacccat
    28201 actggcaatt tgtgcagcat acccagcggt tgcgattctg gcgttatcct cacaacagcc
    28261 gatgcgagca tgatcgtcgc tgcgggcaaa ctgactgcaa aatgctctct ctccgggatc
    28321 tgtggccagt ttttgcatag aggaaacttc ctgatgcgcg aagttttcat ccgccagcca
    28381 ttttttccac gcatactcaa caagactctc cgcccagtcc gtatatcctt cctcaatcag
    28441 tgcatgatga atatcagcat aactaccgcc gcgcgggaaa tggcgcagga accagcgata
    28501 catcaccgct ccgatacgcc attctcgcag gtctgctcgt gtaatgatca ttcgttagtg
    28561 ccggtgatag ccatcgacca tgcttttaaa ggtttcgtgt ggcgtacgcc ccgtggtgca
    28621 aagataaaga tgaccgaaga taaaaaagag gcttataaat gccagagcaa aatgcgcctg
    28681 caataaccag tatcttacgc caggaaacac atctcccacg gcttgcggat agagacacag
    28741 cagccccgtc aatagtaaca acggcagcaa tccatacatg acaccaacat aggcaacctg
    28801 ctgtaaggga ttaaatttag actgggttgt tgccgggaaa ggatgttctt ccccctgcat
    28861 aatgccaaac aaataaaatc gcgtttgttt tgccgcccgc tccagccaac cctgacgacg
    28921 aatgcgatag tggtggccat tacccccaac ggcattgatc agaacaaagc cgagccagca
    28981 tgccagtaac aaaaatccgc aaacttcatg caccgcaacc agacttttaa ccgcagttgc
    29041 gcccaccagc gcaaagtgat ttatcagccc acttgccagc aacaatacaa agagtaacgc
    29101 attcgaccag tgccataatc tgaccgcttt gctgtaaaga taaatcttct caccatgatc
    29161 ggtggctgat ttcttcacgc cacgagcacg aagcaaggcg tgcagcccca gcaccaacca
    29221 catcccaacc agcaatactc cggcaatgat caaccacacc ggccaaaatt cgggagtgaa
    29281 ctgtggcaca tagttcgcta actggctctg aaactgttca gcatgttgcg acgggttcat
    29341 acattttcct ttttaatcaa atgttgaccg aaccggcgat acagatgcgg tttcccggca
    29401 ccaggtagct gatattgata gtatttatta tcctgcaacc acgcctgaat ttctgggcta
    29461 tcttcgcgac caaagatcaa tgcgtgttcc gggcaggcgc tgacgcaaat gggcggaaag
    29521 ccttttgcca gtcgggactc agcgcagaaa tcacatttat ctgcaacttt agtgacaggg
    29581 ttaaggtagc gaacctggta cggacaagcg ccaatgcaat aactgcagcc gatgcactgc
    29641 gatttttcta cccgcacgat cccctgctca tcacgccacg acgcccctgt cgggcatacg
    29701 tcgatgcacg gagcatcttc gcagtgctga catgactggc gaaaaaagtg atactgcgtt
    29761 tcgttgtcgt tatcggtgac tggaatatgt gctatcgata aacggcttcc ctgagcgggg
    29821 acgtggttag ttttacggca tgcgcgggcg caaatattac aaccattgca ccgtgactcg
    29881 tcatgaatca tggcataccg aacttccttt tcttgcctcg tgttcgctaa cagagaagac
    29941 gcagaaccag taaaaaagat tactgttccc atccccagaa caaatttgcg tcgagtgaag
    30001 gacatctgat tatcccctaa tgcagtggct gcaactgtcc accctatcca tgacatctcc
    30061 ctgaaatttc aggggttctg aaactgctct acctcgaacc attcgcataa gccataagcc
    30121 ttgccatagg tcataaatcg ctcgctaaac aagtacaaca ccgccgtcga tgactccacc
    30181 tgccgtatat cggcatattc aggtgcaaca tccatcgcgg cgatcgcggc ttcaatctgc
    30241 gcttcctgga agtaatatgg cgcttgcatc agcatggcga ctttgtaagg acgaggatac
    30301 gtctggcact caaaacgaac ggcgtgtgca atggcgcgac aaatgtcctg ctccacaact
    30361 tgcaaagaca tcgccgcgta gttttcactc atggcctgcg ttgagtaata gtaatcatcc
    30421 tgacttcccg tcatacaagc gatatccgcg tagtctccgt tctgcttaat gctttccagc
    30481 aaaactgaca gttcctcttc tgcaaccgaa tagggtggcg agagaaacac ggcgcgcgcc
    30541 accagctgcc cagccacaga atgctggcga atgaactctg ccaggatttc agccggttct
    30601 ggttccgtta ccgtatcaac ggggtgacgc atcccctgca accattgccg cttcgcctcg
    30661 tcaacttcgc tgactttcgc taacggcaat tcatcctgat ggttcatctt acccattcct
    30721 tacgcaggca gtagattgtg tgctgccaga tcggcggcaa tatcttccag ccccagacgc
    30781 tgcaatgttt cgcgggttgg gcaaccaagc tgtgggtccc agcccatttc tttgtagaac
    30841 atcgtgagcg aagcatgcat atcgtcacga tccattttgt cagtaccttc agtaaagacc
    30901 gggatctgcg gatccttgtc gaatacccag gaacagataa gatcgtgttc attacgcata
    30961 tctttggttt gcatcagttt caccgtgtag gcacgatgca atgtaaagat acgctctgca
    31021 gctaaatcta atttttcctg agtcatctct tcgccagtga tcgctttgaa gaatttggct
    31081 tcaagcgcca gatcgccccg gtaattacgg cttttcagcg gcgaaacggt cattggccag
    31141 acccagttgc acagggtgac ggcgttatgc aaacagaccc gcaacagcga ccacttagca
    31201 tatttaattt ttgcgtcatt gatcggcgtg tagtttttgg tttcatcgta agcatcttca
    31261 gatccaaaaa gttctttcgc cacttcacgt tgcagtttca atggcaagcc ggaaccaata
    31321 aagttgatat gggtatgcgt catgcagtca cggttgaaca tacagttaac aatggaaccg
    31381 acctgcgctg acgcttcatt ggcatggtga accggatagc caaacggcga ccagagttta
    31441 tttttcgcgt agccccagta ctcttcaccc aaattccagc gttctgcgat ggcatatgaa
    31501 ccatcagcca ggtggctcag ctcacccaca cgatgcgcca gacggtagta aaaatcttta
    31561 atgaagttaa cgtcacccgc ttccagttga tcccagcgaa tttccgcata ctcttcagct
    31621 ggcagaacac gcttgaacac acctttgctg taacaataag taaagtcgcg atgcagctgc
    31681 ccgtagttac accacaggcc gtagtcatcg aacaaattca gaccaaccag gttaccaatc
    31741 acacgaccat cgtctttatc ttcaaaatct ttcggcccgt tcgggaagat ggtggtatgg
    31801 acaaagtttg ctacacaagt gttaccacct gtactgggca cgccaaactc ttttacccga
    31861 ggaatattca tttgggtcat acaacggatc gggcaagagt ggcagccgct catttttact
    31921 gtgtatttct ctgccgccgg acctaagtca aaaacggatt tataggtacg aaagccgacc
    31981 gtattctgat tgcctggcgg aatttcaccc gtttcaattg gaccgccttc agccgcgccc
    32041 caaaacagcc ctttacgtgc tgtccagcgt gacttgggat ctgaatactc tgcccacgat
    32101 tgcggcgtac ttggcacgac atggttgtta ttcgcaccga taagttcagt catcatgtaa
    32161 tcattgagac gcttcatctc ctgacgatcg gcaatgttga cccctttcgt cccttcaacc
    32221 gcaatcgctt tcaggttttt cgaacccatt attgcgccag ttcccgcacc accgctgtgg
    32281 ttacggctat tcaacatgcc agagagagga acaaggtttt ccccagcctg accaatagcc
    32341 gccacacagg tttccggact ggtcaatcga caaatttctt ccgtcgttgc gcgcgtccct
    32401 tttccccata agaaatcggc tttttccagg ctaactttgt catctttaat cttcagccat
    32461 accggtgatt tcgccttccc ttcgataata atgacgtcgt atccagcgaa tttcatttgc
    32521 gctgcaaaaa agccccccat atgggcatcg acgactaaat ttcctttggt aaaagtagaa
    32581 agtgaggtga tatttacgcg agaactacag ggggcaccag atccagttaa tgggccggta
    32641 gcaaagacta atttattcgc ttcatcgaaa ggtttcgtgc ctggcggtac ttcgtcatac
    32701 ataattttgt agccgaagcc catgccaccg acaaaacttt taaacttact ggaatcttcg
    32761 agggtaatat ttcctgtcgt gagattgact cttaatatat tacctgtcca accgttagcc
    32821 atgatttttt cctttgcaag attcacacag taatatcttt ccactcgata atttttaacg
    32881 cccctgttgg gcaggcgttt gcgcattcac cgcataacac acatttcgag gattttttac
    32941 tttcggtatt tacggtggcc atcatccacg ggcatgccgt agtacaggcg ctacagccaa
    33001 tacaacgttt atgatcgacg gtaatacagc cttctttctg ctgccaggta atcgcaccaa
    33061 tcgggcagac gttcatgcat tgcggctctt tgcattgacg gcaggtgtcc gcggtatagt
    33121 tgagatcgcc atacaggccg ccgccagagc caaccccgtt gtcgccaaag aaataattgc
    33181 gatggatttt gatacgggag aagaatgtcc ctactgagcc atcgttgaag ttggtacaag
    33241 agatttcaca gcggtggcaa cccgtgcatc gcgctcgctg ggtcaccagc acccctttgg
    33301 gtgtatttat caaccctact gtgccgctat caatatcttc ctgtttgcaa cccaaaagtg
    33361 acagtaacgc aggggcaatt gttagaccag taaggccttt tcctgatata cgcaagaatt
    33421 ctaatcgcgt taagccaata tctaatagtg gacgatcaac cgggttcatt tatttcgcat
    33481 cctcttcgca aacacagtgc tgacaattca gcagcctgat attaagcatt caactaatac
    33541 gctgctgact ctatccccaa caccagatat tttccctaac ccgatagtgt atgagaaaag
    33601 atttaacgcc gataaactaa tcctttatgg ttaggagaaa
//
LOCUS       DAZKFU020000020        33166 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZKFU020000020 DAZKFU020000000
VERSION     DAZKFU020000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.020"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            58..822
                     /gene="puuD"
                     /locus_tag="ACHOQQ_RS03850"
                     /old_locus_tag="pgaptmp_000770"
     CDS             58..822
                     /gene="puuD"
                     /locus_tag="ACHOQQ_RS03850"
                     /old_locus_tag="pgaptmp_000770"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGU4469065.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            849..1406
                     /gene="puuR"
                     /locus_tag="ACHOQQ_RS03855"
                     /old_locus_tag="pgaptmp_000771"
     CDS             849..1406
                     /gene="puuR"
                     /locus_tag="ACHOQQ_RS03855"
                     /old_locus_tag="pgaptmp_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGU4469066.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            1556..3043
                     /gene="puuC"
                     /locus_tag="ACHOQQ_RS03860"
                     /old_locus_tag="pgaptmp_000772"
     CDS             1556..3043
                     /gene="puuC"
                     /locus_tag="ACHOQQ_RS03860"
                     /old_locus_tag="pgaptmp_000772"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGU4469067.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDLDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            3045..4325
                     /gene="puuB"
                     /locus_tag="ACHOQQ_RS03865"
                     /old_locus_tag="pgaptmp_000773"
     CDS             3045..4325
                     /gene="puuB"
                     /locus_tag="ACHOQQ_RS03865"
                     /old_locus_tag="pgaptmp_000773"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGU4469068.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTTKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            4363..5628
                     /gene="puuE"
                     /locus_tag="ACHOQQ_RS03870"
                     /old_locus_tag="pgaptmp_000774"
     CDS             4363..5628
                     /gene="puuE"
                     /locus_tag="ACHOQQ_RS03870"
                     /old_locus_tag="pgaptmp_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGU4469069.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHDVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5748..6725)
                     /gene="pspF"
                     /locus_tag="ACHOQQ_RS03875"
                     /old_locus_tag="pgaptmp_000775"
     CDS             complement(5748..6725)
                     /gene="pspF"
                     /locus_tag="ACHOQQ_RS03875"
                     /old_locus_tag="pgaptmp_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGU4469070.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIILDPFKRRPPEEANAVSENTSLPMLP
                     LDLREFQMQQEKELLQHSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            6892..7560
                     /gene="pspA"
                     /locus_tag="ACHOQQ_RS03880"
                     /old_locus_tag="pgaptmp_000776"
     CDS             6892..7560
                     /gene="pspA"
                     /locus_tag="ACHOQQ_RS03880"
                     /old_locus_tag="pgaptmp_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGU4469071.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            7614..7838
                     /gene="pspB"
                     /locus_tag="ACHOQQ_RS03885"
                     /old_locus_tag="pgaptmp_000777"
     CDS             7614..7838
                     /gene="pspB"
                     /locus_tag="ACHOQQ_RS03885"
                     /old_locus_tag="pgaptmp_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGU4469072.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            7838..8197
                     /gene="pspC"
                     /locus_tag="ACHOQQ_RS03890"
                     /old_locus_tag="pgaptmp_000778"
     CDS             7838..8197
                     /gene="pspC"
                     /locus_tag="ACHOQQ_RS03890"
                     /old_locus_tag="pgaptmp_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGU4469073.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            8206..8427
                     /gene="pspD"
                     /locus_tag="ACHOQQ_RS03895"
                     /old_locus_tag="pgaptmp_000779"
     CDS             8206..8427
                     /gene="pspD"
                     /locus_tag="ACHOQQ_RS03895"
                     /old_locus_tag="pgaptmp_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGU4469074.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            8502..8816
                     /gene="pspE"
                     /locus_tag="ACHOQQ_RS03900"
                     /old_locus_tag="pgaptmp_000780"
     CDS             8502..8816
                     /gene="pspE"
                     /locus_tag="ACHOQQ_RS03900"
                     /old_locus_tag="pgaptmp_000780"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGU4469075.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            9026..10705
                     /gene="ycjM"
                     /locus_tag="ACHOQQ_RS03905"
                     /old_locus_tag="pgaptmp_000781"
     CDS             9026..10705
                     /gene="ycjM"
                     /locus_tag="ACHOQQ_RS03905"
                     /old_locus_tag="pgaptmp_000781"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HGU4469076.1"
                     /translation="MKQEITDYLAEIYGGTFTSTHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSGKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSEEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSN
                     AEGNCLTGLFNVSKNIQHVNITGLHGRDLISEVDILGNKITLRPWQVMWIK"
     gene            10719..12011
                     /gene="ycjN"
                     /locus_tag="ACHOQQ_RS03910"
                     /old_locus_tag="pgaptmp_000782"
     CDS             10719..12011
                     /gene="ycjN"
                     /locus_tag="ACHOQQ_RS03910"
                     /old_locus_tag="pgaptmp_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4469077.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQASQKKLDELIEQH"
     gene            12032..12913
                     /gene="ycjO"
                     /locus_tag="ACHOQQ_RS03915"
                     /old_locus_tag="pgaptmp_000783"
     CDS             12032..12913
                     /gene="ycjO"
                     /locus_tag="ACHOQQ_RS03915"
                     /old_locus_tag="pgaptmp_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4469078.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            12900..13742
                     /gene="ycjP"
                     /locus_tag="ACHOQQ_RS03920"
                     /old_locus_tag="pgaptmp_000784"
     CDS             12900..13742
                     /gene="ycjP"
                     /locus_tag="ACHOQQ_RS03920"
                     /old_locus_tag="pgaptmp_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4469079.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            13773..14825
                     /gene="ycjQ"
                     /locus_tag="ACHOQQ_RS03925"
                     /old_locus_tag="pgaptmp_000785"
     CDS             13773..14825
                     /gene="ycjQ"
                     /locus_tag="ACHOQQ_RS03925"
                     /old_locus_tag="pgaptmp_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGU4469080.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPAGAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            14843..15631
                     /gene="ycjR"
                     /locus_tag="ACHOQQ_RS03930"
                     /old_locus_tag="pgaptmp_000786"
     CDS             14843..15631
                     /gene="ycjR"
                     /locus_tag="ACHOQQ_RS03930"
                     /old_locus_tag="pgaptmp_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU4469081.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEVLAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            15641..16696
                     /gene="ycjS"
                     /locus_tag="ACHOQQ_RS03935"
                     /old_locus_tag="pgaptmp_000787"
     CDS             15641..16696
                     /gene="ycjS"
                     /locus_tag="ACHOQQ_RS03935"
                     /old_locus_tag="pgaptmp_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4469082.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVNPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQARVMRDTARRQGKVLAYDFHHRFALDTQQLREQVTNGILGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            16693..18960
                     /gene="ycjT"
                     /locus_tag="ACHOQQ_RS03940"
                     /old_locus_tag="pgaptmp_000788"
     CDS             16693..18960
                     /gene="ycjT"
                     /locus_tag="ACHOQQ_RS03940"
                     /old_locus_tag="pgaptmp_000788"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HGU4469083.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLCLLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGLSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHREDE"
     gene            18957..19616
                     /gene="pgmB"
                     /locus_tag="ACHOQQ_RS03945"
                     /old_locus_tag="pgaptmp_000789"
     CDS             18957..19616
                     /gene="pgmB"
                     /locus_tag="ACHOQQ_RS03945"
                     /old_locus_tag="pgaptmp_000789"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGU4469084.1"
                     /translation="MKLQGVVFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGDKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLCAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDFLTWSRLSAFWQN
                     V"
     gene            19630..20712
                     /locus_tag="ACHOQQ_RS03950"
                     /old_locus_tag="pgaptmp_000790"
     CDS             19630..20712
                     /locus_tag="ACHOQQ_RS03950"
                     /old_locus_tag="pgaptmp_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4469085.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKERNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            20757..21662
                     /gene="ompG"
                     /locus_tag="ACHOQQ_RS03955"
                     /old_locus_tag="pgaptmp_000791"
     CDS             20757..21662
                     /gene="ompG"
                     /locus_tag="ACHOQQ_RS03955"
                     /old_locus_tag="pgaptmp_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HGU4469086.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(21713..22711)
                     /gene="ycjW"
                     /locus_tag="ACHOQQ_RS03960"
                     /old_locus_tag="pgaptmp_000792"
     CDS             complement(21713..22711)
                     /gene="ycjW"
                     /locus_tag="ACHOQQ_RS03960"
                     /old_locus_tag="pgaptmp_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4469087.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPHQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMSLK"
     gene            22866..24263
                     /gene="ycjX"
                     /locus_tag="ACHOQQ_RS03965"
                     /old_locus_tag="pgaptmp_000793"
     CDS             22866..24263
                     /gene="ycjX"
                     /locus_tag="ACHOQQ_RS03965"
                     /old_locus_tag="pgaptmp_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGU4469088.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            24260..25321
                     /gene="ycjF"
                     /locus_tag="ACHOQQ_RS03970"
                     /old_locus_tag="pgaptmp_000794"
     CDS             24260..25321
                     /gene="ycjF"
                     /locus_tag="ACHOQQ_RS03970"
                     /old_locus_tag="pgaptmp_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGU4469089.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQVQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            25469..27010
                     /gene="tyrR"
                     /locus_tag="ACHOQQ_RS03975"
                     /old_locus_tag="pgaptmp_000795"
     CDS             25469..27010
                     /gene="tyrR"
                     /locus_tag="ACHOQQ_RS03975"
                     /old_locus_tag="pgaptmp_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGU4469090.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(27054..27560)
                     /gene="tpx"
                     /locus_tag="ACHOQQ_RS03980"
                     /old_locus_tag="pgaptmp_000796"
     CDS             complement(27054..27560)
                     /gene="tpx"
                     /locus_tag="ACHOQQ_RS03980"
                     /old_locus_tag="pgaptmp_000796"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGU4469091.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            27679..28644
                     /gene="ycjG"
                     /locus_tag="ACHOQQ_RS03985"
                     /old_locus_tag="pgaptmp_000797"
     CDS             27679..28644
                     /gene="ycjG"
                     /locus_tag="ACHOQQ_RS03985"
                     /old_locus_tag="pgaptmp_000797"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGU4469092.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQQILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFNLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEQALQFTTGELHL"
     gene            complement(28619..29347)
                     /gene="mpaA"
                     /locus_tag="ACHOQQ_RS03990"
                     /old_locus_tag="pgaptmp_000798"
     CDS             complement(28619..29347)
                     /gene="mpaA"
                     /locus_tag="ACHOQQ_RS03990"
                     /old_locus_tag="pgaptmp_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGU4469093.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(29638..30276)
                     /locus_tag="ACHOQQ_RS03995"
                     /old_locus_tag="pgaptmp_000799"
     CDS             complement(29638..30276)
                     /locus_tag="ACHOQQ_RS03995"
                     /old_locus_tag="pgaptmp_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4469094.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(30297..31226)
                     /locus_tag="ACHOQQ_RS04000"
                     /old_locus_tag="pgaptmp_000800"
     CDS             complement(30297..31226)
                     /locus_tag="ACHOQQ_RS04000"
                     /old_locus_tag="pgaptmp_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4469095.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHL"
     gene            complement(31226..32146)
                     /locus_tag="ACHOQQ_RS04005"
                     /old_locus_tag="pgaptmp_000801"
     CDS             complement(31226..32146)
                     /locus_tag="ACHOQQ_RS04005"
                     /old_locus_tag="pgaptmp_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4469096.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            32284..>33166
                     /gene="pgrR"
                     /locus_tag="ACHOQQ_RS04010"
                     /old_locus_tag="pgaptmp_000802"
     CDS             32284..>33166
                     /gene="pgrR"
                     /locus_tag="ACHOQQ_RS04010"
                     /old_locus_tag="pgaptmp_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGU4469097.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSYPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLRGYHLYY
                     PHRRHAGSAFSLFIDRLK"
BASE COUNT         8039 a         8177 c         8937 g         8013 t
ORIGIN      
        1 aaaggtcatt taaaccgcag taatggcgat aatttagtcc actttgtgag attgagcatg
       61 gaaaatataa tgaacaatcc ggttatcggt gtcgtaatgt gcaggaacag gcttaagggt
      121 catgcgaccc agactctgca agaaaagtac ctgaatgcca tcatccatgc aggcggcttg
      181 ccaattgcgc tgccacatgc gctggcggaa ccgtccttac ttgaacaact tttgccgaaa
      241 ctcgatggca tttatcttcc tggtagtccc agcaatgtgc agccgcacct atatggtgaa
      301 aacggcgatg agcctgacgc cgatcccggg cgtgatcttc tgagcatggc gttaattaat
      361 gccgcactcg aaaggcgcat ccccattttc gccatctgcc ggggtttaca ggaactggtg
      421 gtggcaaccg gtggttcgtt gcatcgcaag ctgtgcgaac agcctgaatt gctggaacat
      481 cgggaagatc ccgaactgcc ggtggaacag caatatgcac cgtcgcatga agttcaggtt
      541 gaagaggggg gattactgtc tgcgttgtta cctgaatgta gcaacttttg ggtaaactct
      601 ctgcatggac aaggggcgaa ggtcgttagc ccacggttgc gtgttgaagc tcgctcgccg
      661 gatggcttag ttgaggcggt tagcgtcatc aatcatcctt ttgcgctggg cgtacagtgg
      721 cacccggaat ggaacagtag cgagtacgcg ctttcgcgta tattgttcga gggctttatc
      781 accgcttgtc agcaccatat cgctgaaaaa cagcgactct gaccactaca gtttaaggaa
      841 atgcaaatat gagtgatgag ggactggcgc caggaaaacg cttgtcggaa atccgccagc
      901 agcaggggct ttcacaacgt cgtgccgccg aactctccgg gctgactcac agtgctatca
      961 gtacgataga acaagataaa gtcagccctg ccatcagtac gctgcaaaag ctgctgaagg
     1021 tgtatggtct gtcactctcg gaattctttt ccgagccgga aaaacctgat gagccgcagg
     1081 tcgtcattaa tcaggacgat ttaattgaga tgggcagtca gggtgtgtca atgaagctgg
     1141 ttcataacgg taacccgaat cgcacgctgg cgatgatctt tgaaacgtac cagccgggca
     1201 caaccactgg ggaaagaatt aagcatcagg gtgaggaaat aggcactgta ctggaaggtg
     1261 aaattgttct gacgattaat ggtcaggatt accatctcgt cgcggggcaa agttatgcca
     1321 ttaataccgg catcccgcac agtttcagta atacatcggc aggtatttgc cgaattatta
     1381 gcgcccatac gcccaccacg ttttaatctt tttgttctgt aagccgggta agcgcaatgc
     1441 gcccggcaat ccataaagaa tgtcgccaga aataaacggg catacggccc ggggatctct
     1501 gcgccctgac gttcacaaac tgcatatatc tgatagtcgt gaaacaggag tcataatgaa
     1561 ttttcatcat ctggcttact ggcaggataa agcgttaagt ctcgccattg aaacccgctt
     1621 atttattaac ggtgaatata ctgctgcggc ggaaaatgaa acctttgaaa ccgttgatcc
     1681 ggtcacccag gcaccgctgg cgaaaattgc ccgcggcaag agcgtcgata tcgaccgtgc
     1741 ggtgagcgca gcacgcggcg tatttgaacg cggcgactgg tcactctctt ctccggctaa
     1801 acgtaaagcg gtgctgaata aactcgccga tttaatggaa gccaacgccg aagagctggc
     1861 actgctggaa actctcgaca ccggcaaacc gattcgtcac agtctgcgag atgatattcc
     1921 cggcgcggcg cgcgccattc gctggtacgc cgaagcgatc gacaaagtgt atggcgaagt
     1981 ggcgaccacc agtagccatg agctggcgat gatcgtgcgt gaaccggtcg gcgtaattgc
     2041 cgccattgta ccgtggaact tcccgctgct gctgacttgc tggaagcttg gcccggcgct
     2101 ggcggcggga aacagcgtga ttctaaaacc gtctgaaaaa tcaccgctca gtgcgattcg
     2161 tctcgcgggg ctggcgaaag aagcaggctt gccggatggt gtgttgaacg tagtaacggg
     2221 ttttggtcat gaagcggggc aggcgctgtc gcgccataac gatatcgatg ccatcgcctt
     2281 taccggttcg acccgtaccg ggaaacagct gctgaaagat gcgggcgaca gcaacatgaa
     2341 acgcgtctgg ctggaagcag gtggcaaaag cgccaacatc gttttcgctg actgcccgga
     2401 tctgcaaaaa gcggcgagtg cgaccgctgc gggcattttc tacaaccagg ggcaggtgtg
     2461 tatcgccgga acgcgcctgt tgctggaaga gagcatcgcc gatgaattct tagccctgtt
     2521 aaaacagcag gcgcaaaact ggcagccggg ccatccactt gatcccgcaa ccaccatggg
     2581 caccttaatc gactgcgccc acgccgactc ggtccatagc tttattcggg aaggcgaaag
     2641 caaagggcaa ctgttgttgg atggccgtaa cgccgggctg gctgccgcca tcggcccgac
     2701 catctttgtg gatttagacc cgaatgcgtc cttaagccgc gaagagattt tcggtccggt
     2761 gctggtggtc acgcgtttca catcagaaga acaggcgcta cagcttgcca acgacagcca
     2821 gtacggcctt ggcgcggcgg tatggacgcg cgacctctcc cgcgcgcacc gcatgagccg
     2881 acgcctgaaa gccggttccg tcttcgtcaa taactacaac gacggcgata tgaccgtgcc
     2941 gtttggcggc tataagcaga gcggcaacgg tcgcgacaaa tccctgcatg cccttgaaaa
     3001 attcaccgaa ctgaaaacca tctggataag cctggaggcc tgaaatgacc gaacatacca
     3061 gcagttacta cgccgccagt gcgaataaat acgcgccatt cgacacactg aatgagtcaa
     3121 tcacctgcga cgtatgcgtg gtcggcggcg gctataccgg gctgtcttcc gcgctacatc
     3181 tggcggaagc gggctttgac gtagtggttc tcgaagcctc acgcatcggc tttggcgcca
     3241 gcggacgcaa cggggggcag cttgtgaact cctacagccg cgacatcgac gtgatcgaaa
     3301 aaagctacgg catggacacc gcccgtatgc tcggcagcat gatgttcgag ggtggcgaga
     3361 tcatccgcga acgtatcaaa cgctatcaga ttgactgcga ctaccgcccc ggcggcctgt
     3421 ttgtggcgat gaatgataaa cagctcgcca cactcgaaga gcagaaagag aactgggaac
     3481 gctacggcaa taaacagctg gagctgctgg acgccaacgc cattcgccgc gaggtcgcca
     3541 gcgatcgcta cactggtgcg ctgctggatc acagcggcgg gcatattcac ccgctgaacc
     3601 tcgccattgg tgaagcggac gctatccgcc tgaacggcgg gcgcgtgtat gagctttctg
     3661 cggtgacgca gatccagcac accacgcccg ccgtggtgcg tactaccaaa ggccaggtga
     3721 cggcgaagta tgtgattgtc gccgggaatg cgtacctggg cgataaagtg gagccggaac
     3781 tggcgaagcg tagtatgccg tgcggcacgc aggtgatcac cactgagcgg ctgtcagaag
     3841 atttagcccg ttcgctgatc ccgaaaaact actgtgtgga agactgtaac tatctgcttg
     3901 attactaccg tcttaccgcc gacaaccgtt tgctgtacgg cggcggcgtg gtgtacggcg
     3961 cgcgcgaccc ggatgacgtt gagcgtcttg tggtgccgaa actgctgaaa accttcccgc
     4021 agttgaaggg cgtgaaaatc gactatcgct ggacgggcaa cttcctgctg accctgtcgc
     4081 gtatgccgca gttcggtcgc ctcgatacca acatctatta catgcagggt tacagcggcc
     4141 acggcgtcac ctgtactcat cttgccggac gtttgattgc cgaactgctg cgcggcgacg
     4201 ccgaacgttt cgatgccttc gccaatctgc cgcattaccc gttccccggc gggcgcacgc
     4261 tgcgtgtgcc gtttaccgcg atgggcgcgg cttattacag cctgcgcgat cgtctgggcg
     4321 tttaatttcc gattaacagt gaagagtcaa aaggtgtgaa acatgagcaa caatgaattc
     4381 catcagcgtc gtctttctgc caccccgcgc ggggttggcg tgatgtgtaa cttcttcgcc
     4441 cagtcggctg aaaacgctac gctgaaggat gttgagggca acgagtacat cgatttcgcc
     4501 gcaggcattg cggtgctgaa taccggacat cgccaccctg atctggtcgc ggcggtggag
     4561 cagcaactgc aacagtttac ccacaccgcg tatcagattg tgccgtatga aagctacgtc
     4621 accctggcgg agaaaatcaa cgcccttgcc ccggtaagcg ggcaggctaa aaccgcgttc
     4681 ttcaccaccg gtgcggaagc ggttgagaac gcggtgaaaa tcgcccgcgc ccataccgga
     4741 cgcccaggtg tgattgcgtt tagcggcggc ttccacggtc gtacgtacat gaccatggcg
     4801 ctgaccggaa aggtcgcgcc gtacaaaatc ggcttcggcc cgttccccgg ttcggtgtat
     4861 cacgtgccgt atccgtcaga tttacatgac gtttcaacgc aggactccct ggacgccatc
     4921 gaacgtttgt ttaaatcaga catcgaagcg aaacaggtcg cggcgattat ttttgaaccg
     4981 gttcagggcg agggcggttt caacgtggcg ccaaaagaat tggttgccgc cattcgccgc
     5041 ctgtgcgacg aacacggtat tgtgatgatt gccgatgaag tgcaaagcgg ctttgcgcgt
     5101 accggcaaac tgtttgcgat ggatcattac gccgataagc cggacttaat gacgatggcg
     5161 aaaagcctcg cgggcgggat gccgctttcg ggcgtggtcg gtaacgcgaa tattatggac
     5221 gcacccgcgc cgggcgggct tggcggcacc tacgccggta acccgctggc ggtggctgcc
     5281 gcgcacgcgg tgctcaacat tatcgacaaa gaatcactct gcgaacgcgc gaatcaactg
     5341 ggccagcgtc tgacaaacac gctgattgat gccaaagaaa gcgttccggc catcgcggcg
     5401 gtacgcggtc tggggtctat gattgcggca gagtttaacg atccgcaaac gggcgagccg
     5461 tcagcggcga ttgcacagaa aatccagcaa cgcgcgctgg cgcaggggct gcttctgctg
     5521 acctgtggcg catacggcaa cgtgattcgt ttcctgtatc cgctgaccat cccggatgcg
     5581 caattcgatg cggcaatgaa aattttgcag gatgcgctga gcgattaata gcccaacgca
     5641 ataatgtctg atgcgctgcg cttatcaggc ctgcaaacgg cgtattgatt atgtatgccg
     5701 gataaagcat tcacgccgca tccggcaagt tgtattgccc aacttcgcta aatctggtgc
     5761 tttttcaaca acgcgcggaa ctgatgatag gttaacccca gcaattcagc tgcgcgcttc
     5821 tggttatatt tcccttgttg caaactgtgt tgcagcaact ctttttcctg ctgcatctga
     5881 aactcacgta aatccagcgg cagcatcgga agcgaagtgt tttctgaaac ggcgttggct
     5941 tcttctggtg gacgccgttt aaagggatca agaatgatgt catcaagcgg ataatcgctg
     6001 gtgccgtggc gatacactga acgttccacc acgtttttca attcacgaat atttcccggc
     6061 cagcgataat tcagcaatgt ttctctggcg tgctcggtaa aacccgggaa cagaggcagc
     6121 ttgatttccc gacacatctg gatggcaaag tgttctgcca tcaacataat gtcgctttcg
     6181 cgctcgcgca gtggtggcag ttgtacaaca tcaaaagcca gtcggtcgag caggtctgcg
     6241 cgaaaagtgc cttcattgac catcgccggg agatcggcat tcgtcgcgca taccaaccgc
     6301 acattcacct gcaatggctg actaccgcct acgcgctcca gttcaccgta ctcaatcacg
     6361 cgcaataatt tttcctgcac catcatcggt gccgtagcga gttcatcaag aaatagcgta
     6421 ccgccgtcgg cacgttcaaa tctccctgga tgacgttttt gcgcaccggt aaacgcgcca
     6481 gcttcgtgac caaacagttc ggaatccagc agattttcat ttaacgccgc gcagttaagg
     6541 gaaataaacg gcccttgcca acgggaggag agataatgca gacggctggc gatcagttct
     6601 ttacctgtgc cgcgctcgcc gatgatgagc accggtttgt ccagcggtgc gagatgcgaa
     6661 acctgttcca gcacttcgag aaagctgttc gcctcaccga gtaaattatc tttgtattct
     6721 gccatgatga aattcgccac ttgttagtgt aattcgctaa ttcatcctgg catgttgctg
     6781 ttgattcttc aatcagatct ttataaatca aaaagataaa aaattggcac gcaaattgta
     6841 ttaacagttc agcaggacaa tcctgaacgc agaaatcaag aggacaatat tatgggtatt
     6901 ttttctcgct ttgccgacat cgtgaatgcc aacatcaacg ctctgttaga gaaagcggaa
     6961 gatccacaga aactggtgcg tctgatgatc caggagatgg aagatacatt ggttgaagta
     7021 cgttctactt cggcgcgtgc gctggcggaa aagaaacagc tgactcgccg tattgaacaa
     7081 gcgtcggcgc gtgaagttga atggcaggaa aaagccgaac tggcactact gaaagagaga
     7141 gaagatctgg cgcgtgcggc gttaattgaa aaacagaaac tgaccgatct gattaagtcc
     7201 ctggaacatg aagtgacgct ggtggacgat acgctggtgc gtatgaagaa agagatcggc
     7261 gagctggaaa acaaattgag cgaaacccgt gctcgccagc aggcattgat gttacgccat
     7321 caggcggcaa actcgtcgcg cgatgtgcgt cgtcagctgg acagtggcaa actggatgaa
     7381 gcaatggctc gtttcgaatc tttcgaacgt cgaattgacc agatggaagc ggaagcagaa
     7441 agccacagct tcggtaaaca aaaatcgctg gacgatcagt ttgccgaact gaaagccgat
     7501 gatgcaatca gcgagcaact ggcacaatta aaagccaaaa tgaagcaaga caatcaataa
     7561 taacatccag gcggcgtccg aacgcgccgc cgctcatcgt ctaaggagta cttatgagcg
     7621 cgctatttct ggctattcca ttaaccattt ttgtgctgtt tgttttaccg atctggttat
     7681 ggctgcatta cagcaatcgt tctggtcgca gtgaattgtc gcaaagtgag cagcagcgat
     7741 tagcgcaact ggctgatgaa gcaaaacgga tgcgcgaacg tattcaggcg ctggaatcta
     7801 ttcttgatgc agaacatccg aactggaggg atcgctaatg gcgggcatta atctcaataa
     7861 aaaattatgg cgtattccac agcagggcat ggtccgtggc gtctgcgccg ggattgccaa
     7921 ctattttgat gtaccggtaa aactggtgcg tatcctggtg gtgctgtcga ttttcttcgg
     7981 tctggcgctg tttaccctgg ttgcttacat cattttgtca tttgcgcttg atccaatgcc
     8041 ggacaacatg gcctttggtg agcagctacc ttccagcagc gaattgctgg atgaagtcga
     8101 ccgtgaactg gcggcaagtg aaacgcgttt acgcgagatg gaacgttatg tcacttccga
     8161 tactttcacg ttacgtagcc gtttccgtca actgtgagga aagttatgaa tactcgctgg
     8221 caacaggccg ggcaaaaggt aaagcctggt ttcaaattag caggcaagct ggtacttctt
     8281 accgcactgc gctatggccc agcgggtgtg gcgggctggg cgataaaatc agttgctcgc
     8341 cgaccgctga aaatgttgct ggctgtggcg ctggaaccgc tgttaagtcg ggctgccaat
     8401 aaactggcgc agcgttataa aaggtgaggg gagtttcgca aaaattgtta aatctcaggc
     8461 gtataatgga tggcaatttt catccataga aggacgctta catgtttaaa aaaggcttac
     8521 ttgctctgac actggtgttt tcactgcccg ttttcgccgc tgaacactgg atcgatgttc
     8581 gtgttccaga acagtatcag caggagcacg ttcagggggc catcaatatt cccctgaagg
     8641 aagtgaaaga gcgcattgcc accgccgttc cggataaaaa cgacaccgtg aaagtgtatt
     8701 gcaatgccgg acgccagtcc gggcaggcaa aagagatcct tagcgagatg ggatataccc
     8761 acgttgagaa cgccggtggc ctgaaagaca tcgcaatgcc gaaggtcaaa ggttaaaaga
     8821 attcaccatg agcggcgctt atgcgccgtt tttttctgtc acaccttatt tacatcccta
     8881 tagattattt gcgtcagctc acaaatacgc tttttccctg gtaaaaaatg atttcctgcg
     8941 tgactaaaac ccttgtgctc aattgacagt ttattttctg cggagtagtc tctcgtttca
     9001 tgggaccgct accacggaaa ggcacatgaa acaggaaatt acggattact tggcagaaat
     9061 ttacggtgga acatttacct caactcattt acagaaactt gtaacgcgtc ttgagagtgc
     9121 gaaacgatta attacacagc gacgtaaaaa acactgggat gaaagtgacg tcgtgttaat
     9181 tacctatgcc gatcaatttc acagtaatga tttaaaacca ctacccacat ttaatcagtt
     9241 ttaccatcaa tggctgcaaa gcattttttc acatgttcat ttattacctt tttatccatg
     9301 gtcatctgat gatggatttt cggtaattga ttatcatcag gtcgccagtg aagcggggga
     9361 gtggcaggat attcagcagc tcggtgaatg cagtcattta atgtttgatt ttgtctgcaa
     9421 ccatatgtcg gcaaaaagtg aatggtttaa aaactatttg caacagcttc cgggttttga
     9481 agatttcttt attgcggttg atccgcaaac cgatctcagt gccgtcaccc gtccgcgtgc
     9541 gttaccgcta ttaacgccgt ttcaaatgga tgataattcc acgcgccatt tatggaccac
     9601 ctttagcgac gatcaaattg acctcaatta ccgtagccct gaagtgttgc tggcgatggt
     9661 ggatgtattg ctgtgctacc tggaaaaggg cgcagagtat gtccgtctgg atgccgttgg
     9721 ctttatgtgg aaagagccgg gaacaagctg catccatctg gaaaaaacac atctgattat
     9781 caaactgtta cggtcgatta ttgataacgt cgcgccaggc acagtgatca ttaccgagac
     9841 caatgttccg cataaagaca acattgctta ctttggcaac ggcgatgatg aagcccatat
     9901 ggtgtatcag ttctcgctgc cgccgctggt gctgcatgcg gtgcaaaaac agaacgtcga
     9961 ggcgctgtgc cagtgggcgc aaagtctgag cttgccctcc ggtaaaacga cctggtttaa
    10021 cttcctcgcc tctcacgatg gcatcgggct gaacccactg cggggcttgt taccagaaag
    10081 cgaaatatta gcgctggtcg aggcattaca gcaggaaggt gcattagtaa actggaaaaa
    10141 taatcccgac ggtacgcgca gtccctatga aataaatgtc acctatatgg atgcgttaag
    10201 ccgccgcgag agtagcgatg aagaacgttg cgccaggttt atccttgccc atgcgatttt
    10261 gttaagtttc cccggtgtgc cagcgatata tattcaaagt attctgggct cgcgtaatga
    10321 ttacgcaggt gtcgaaaaac tcggatataa ccgtgcgatt aaccgtaaaa aatatcacag
    10381 tgaagagata acccgagaac tgaacgatga agctacatta aggcatgcgg tatatcatga
    10441 gttgtcgcgt ttaattacac ttcgtcgcag ccataacgag tttcatccgg ataataattt
    10501 taccatcgat acggttaatt catccgtaat gcgtattcaa agaagtaacg cagagggtaa
    10561 ttgtctgacc ggattgttta atgtcagtaa aaatattcag catgtaaata ttactggcct
    10621 gcacggtcgg gatctgatta gtgaagttga tatattgggt aataaaataa cgctgcgccc
    10681 ctggcaggtt atgtggatta aataaaaagg aacatctcat gattaaatca aaaatcgtgc
    10741 tgttatcagc actggtttcc tgcgccctga tttcaggctg taaagaagaa aataaaaccc
    10801 atgtatccat cgaatttatg cattcttcgg tggagcagga gcgtcaggca gttatcagca
    10861 aattgattgc ccgttttgaa aaagaaaacc caggtatcac cgttaaacaa gtgcccgtgg
    10921 aagaagacgc ttataacacc aaagtcatca ccctttcacg tagcggctcg ctgccggaag
    10981 tgatcgaaac cagccatgac tacgccaaag ttatggacaa agagcagctt atcgatcgca
    11041 aggcggttgc cacggtcatc agcaacgttg gtgaaggcgc gttttacgat ggcgtactgc
    11101 gtattgtgcg caccgaagat gggagtgcct ggaccggtgt tcccgtcagc gcctggattg
    11161 gcggcatctg gtatcgcaaa gatgtgctgg caaaagccgg gctggaggag ccgaaaaact
    11221 ggcaacagct gctggacgtt gcacagaaac tgaatgaccc ggcgaataaa aaatacggta
    11281 ttgcgctgcc tacggcagaa agcgtgctga cggaacaatc cttctcccag tttgcgttat
    11341 ccaaccaggc caacgtcttt aacgccgaag gcaaaatcac ccttgataca ccagagatga
    11401 tgcaggcgct gacctattac cgcgaccttg ctgccaacac catgccgggt tctaacgaca
    11461 tcatggaagt gaaagacgcc tttatgaacg gcaccgcgcc gatggcgatt tactccacct
    11521 atatccttcc ggctgtaatt aaagaaggcg acccgaaaaa cgtcggtttc gtggtgccaa
    11581 ccgagaaaaa ctctgcggtc tacggcatgt tgacctcgct gaccattacc gccggacaaa
    11641 agaccgaaga gaccgaagca gcagaaaaat ttgtcacctt tatggagcag gcagacaaca
    11701 ttgccgactg ggtgatgatg tcgccaggcg cggcgctgcc ggtgaacaaa gcggttgtca
    11761 ctaccgccac ctggaaagac aacgacgtga tcaaggcgct gggtgaactg ccgaatcagt
    11821 taattggtga actgccaaat attcaggttt ttggcgcagt aggggataaa aactttaccc
    11881 gcatgggtga tgtgacgggt tctggcgtgg tgagttcaat ggtgcataac gtcaccgtgg
    11941 gtaaagccga tctctctact acgctgcaag cgagccagaa aaaactggat gaactgatcg
    12001 aacagcacta aacccaggac aggaatccgc aatgaacagg cttttttcag gtcgttccga
    12061 tatgcccttt gcgctgctgc ttctcgcgcc cagcttatta ctgctgggcg gtctggtggc
    12121 gtggccgatg gtgtcgaata tcgaaatcag ttttttacgt ctgccgctca atcccaacat
    12181 cgagtcaacg tttgttgggg tgagcaacta tgtgcgtatc ctctccgatc ccggcttctg
    12241 gcattcgctg tggatgacgg tctggtatac cgcgctggtg gtggcgggca gcaccgtgct
    12301 cgggctggcg gtggcgatgt ttttcaaccg cgagttccgc ctgcgcaaaa ccgcgcgttc
    12361 gctggtgatc ctctcctacg taacgccgtc catttcgctg gtgttcgcct ggaaatacat
    12421 gttcaacaac ggctacggca ttgtgaacta cctcggcgtc gatcttctgc atctctacga
    12481 gcaggcaccg ctgtggttcg ataacccggg cagtagcttt gtgctggtgg tgctgttcgc
    12541 catctggcgc tatttcccat atgcctttat ctcgtttctg gcgattttgc agaccatcga
    12601 caaatcgctg tacgaagcgg cagagatgga tggcgctaat gcctggcagc ggtttcgcat
    12661 cgtcacgctg cccgcaatta tgccggtcct ggcgacggtg gtgaccctgc gcaccatctg
    12721 gatgttctac atgttcgctg atgtttatct gctgacaacc aaggtcgata ttcttggtgt
    12781 ctatctctac aaaaccgcct ttgcctttaa tgacttaggg aaagcggcgg cgatctcggt
    12841 ggtgctattc atcattattt tcgctgtcat tctgctgacc agaaaacggg tgaacctcaa
    12901 tggcaacaaa taaacgcaca ctcagtcgca tcggttttta ctgcgggctg gcgctgtttc
    12961 tcatcatcac gctatttccg ttttttgtga tgctgatgac ctcgttcaag agcgcaaaag
    13021 aggcgatctc gctgcatcct acgctgctgc cacagcagtg gacgctggag cattacgtcg
    13081 acatttttaa cccggtgatt ttcccgtttg tcgactactt ccgtaacagc atggtggtgt
    13141 cggtggtttc atccgtggtg gcggtatttc tcggtattct cggggcttac gcgctttctc
    13201 gcctgcgctt taaaggtcgg atgaccatca acgccagctt ttacacggtg tacatgttct
    13261 ccggcatttt gctggtggtg ccgctgttca aaattatcac cgcacttggc atttacgaca
    13321 ccgagatggc gctgatcatt accatggtga cgcaaactct gcccaccgcc gtgtttatgc
    13381 tgaaaagtta cttcgacacc atccctgatg agatcgaaga agcggcgatg atggatggcc
    13441 tcaaccgtct gcaaatcatc ttccgtatca ccgtgcccct ggcgatgtcc gggctgatat
    13501 cggtgttcgt ctactgcttt atggtggcgt ggaacgacta tctgtttgcg tcgattttcc
    13561 tctccagcgc cagcaatttc accttaccag tgggcctgaa cgcgctgttc agtacgcctg
    13621 attacatctg gggacggatg atggcggcct cgctggtgac cgcattgccg gtggtgatta
    13681 tgtatgcgct ttcggaacgt tttattaaga gtggtttgac cgccggtggc gtgaagggct
    13741 aaagcggcca gtttctttac aaggagtttt acatgaaaaa gttagtagcc acagcaccgc
    13801 gtgttgctgc gctggttgag tatgaagatc gggcgatttt agccaatgaa gtgaagatcc
    13861 gcgtgcgttt cggcgcaccg aaacacggaa cggaagtggt cgacttccgc gccgccagcc
    13921 cgtttatcga tgaagacttt aacggcgagt ggcagatgtt caccccgcgt ccggcaggtg
    13981 cgccgcgcgg cattgagttt ggcaaattcc agcttggcaa catggtggtt ggcgacatta
    14041 tcgagtgcgg cagcgacgtt accgactatg cggtgggcga cagcgtatgc ggctacggcc
    14101 cgctctccga gacggtcatc attaacgcag tgaataacta caagttgcgc aaaatgccgg
    14161 aaggcagctc ctggaaaaac gccgtctgct acgacccggc gcagttcgcc atgagtggcg
    14221 ttcgcgatgc caacgtacgc gtaggggatt ttgtagtggt ggtagggctt ggcgcgatcg
    14281 gtcaaattgc catccaactg gctaaacgcg ctggcgcgtc ggtggtaatt ggcgtcgatc
    14341 ctattgccca tcgttgtgat attgctcgtc gtcacggtgc ggatttctgc cttaatccca
    14401 ttggcactga cgtaggcaaa gagatcaaaa cgctgaccgg caagcagggt gccgatgtga
    14461 ttatcgaaac cagcggttac gccgacgcgc tgcagtcggc gctgcgcggc ctggcttacg
    14521 gtggcaccat ctcctatgtc gcgtttgcta aaccgtttgc cgaaggtttt aacctcggac
    14581 gcgaagcgca tttcaataac gccaaaattg ttttctcccg cgcgtgcagc gaaccgaacc
    14641 cggattatcc gcgctggagc cgcaagcgta tcgaagaaac ctgctgggag ctgctgatga
    14701 acggttatct caactgtgag gatttaatcg acccggtggt gacctttgct aacagcccgg
    14761 aaagctacat gcagtatgtc gaccagcatc cggaacagag catcaaaatg ggcgtcacgt
    14821 tttaattcaa aggaatgaaa aaatgaaaat aggcacacag aatcaggcgt tcttcccgga
    14881 aaatattctg gagaaatttc gttatatcaa agagatgggc ttcgatggtt ttgagattga
    14941 cggcaaactg ctggtcaaca acatcgaaga agtcaaagcg gcaatcaaag aaaccggttt
    15001 accggtgacc accgcctgcg gtggctatga cggctggatt ggtgacttta tcgaagagcg
    15061 tcgtcttaat ggcttaaagc agatagagcg cattctcgaa gtgctggcag aagtgggcgg
    15121 taaaggtatc gtcgttccgg cagcgtgggg catgtttacc ttccgcttac cgccgatgac
    15181 ctcgccgcgc agcctggacg gcgatcgtaa aatggtgagt gattccctgc gcgtactgga
    15241 acaggtcgcc gcgcgtaccg gaaccgtggt gtatctcgaa ccgttaaacc gttatcagga
    15301 tcacatgatc aacactctcg ccgatgcccg ccgttacatc gtcgaaaacg atcttaaaca
    15361 tgtgcagatt atcggcgatt tctatcacat gaatattgaa gaagataacc tggcgcaggc
    15421 gctgcatgac aaccgcgacc tgctcggtca tgtgcatatt gccgataacc atcgttatca
    15481 gccgggcagc ggcaccctgg atttccacgc gctgtttgaa caactgcgcg ccgataatta
    15541 tcagggctat gtggtgtatg aagggcgcat tcgggcagaa gatcctgccc aggcgtaccg
    15601 tgattctttg gcctggttgc gtacctgcta agaggtcttt gtgaaaagtg caatgacaag
    15661 ctctccgctg cgggtcgcga tcataggcgc gggccaggtg gcggataaag ttcatgcttc
    15721 gtactactgc acccgcaacg atctggaact ggtggctgtc tgtgacagcc gcctttccca
    15781 ggcgcaggcg ctggcagata aatacggtaa tgcatccgtg tgggacgatc cgcaggccat
    15841 gctgctggcg gtgaaccctg acgtggttag cgtctgctca cctaaccgtt ttcactatga
    15901 acataccctt atggcactgg aagccggctg ccatgtgatg tgcgaaaagc cgcctgctat
    15961 gacgccggaa caggctcggg tcatgcgcga taccgcgcgc agacaaggaa aagtactggc
    16021 ctacgacttc catcaccgtt ttgcgcttga tacacaacaa ctgcgtgagc aagtgactaa
    16081 cggcatcctg ggcgaaattt acgtcaccac cgcccgcgcc ctgcgtcgct gcggcgttcc
    16141 cggctggggt gtctttacca ataaagaact gcagggcggt ggcccgctga tcgacatcgg
    16201 tattcatatg ctggatgcgg cgatgtatgt gctgggcttt ccggcggtga aaagcgtgaa
    16261 tgcgcatagc tttcaaaaga tcggcacgca aaagagctgt ggtcaatttg gcgagtggga
    16321 tccggcaact tacagcgtcg aagattcgct gtttggcacc attgaatttc ataacggcgg
    16381 cattctgtgg ctggaaacgt catttgcgct caacatccgc gaacagtcga ttatgaacgt
    16441 cagcttttgt ggtgataaag ccggtgcgac gctgtttcca gcacatatct acaccgataa
    16501 caacggtgag ttaatgacgc tgatgcaacg ggaaatggca gacgacaacc gccatttgcg
    16561 cagcatggaa gcctttatca atcacgtaca gggcaagccc gtgatgatag ccgatgccga
    16621 gcaggggtac atcatccagc aactggtggc ggcgttgtat caatccgcag aaacagggac
    16681 gcgtgtggaa ttatgaccag gccagtaacg ttatcagaac cccatttcag ccagcatacc
    16741 ctgaacaagt atgcatcact gatggcgcag gggaacggct atcttgggct tcgcgccagc
    16801 catgaagaag attacacgcg ccagacgcgc ggcatgtatc tggcggggct gtatcatcgg
    16861 gcgggtaaag gtgaaatcaa cgaactggtg aacctgcccg atgtggtggg gatggagatt
    16921 gccataaatg gtgagatttt ctcattatcc cgcgaagcct ggcagcgtga gcttgacttt
    16981 gccagtggcg aattacgccg cagcgttgtc tggcgtacca gcaacggcac aggttacacc
    17041 atcgccagcc gtcgctttgt ttcggcagac caactgccgc tcattgcgct ggaaatcact
    17101 attactccac tggacgccga cgcgtcagtg ctgatttcaa caggcattga cgccacgcaa
    17161 accaaccacg gtcgccagca tctcgacgaa acccaggtgc gagtgtttgg acagcatttg
    17221 atgcaaggaa tctataccac tcaggatgga cgcagtgatg tggcaattag ctgttgctgc
    17281 caggtgagtg gtgacgttca gcaatgctat acggctaaag agcgccgttt gctgcaacat
    17341 accagtgcgc agcttcatgc aggcgagaca gtgacgttgc aaaaactggt gtggatcgac
    17401 tggcgggatg acaggcaagc cgttttagac gagtggggca gcgcgtcgct ttgcctgctt
    17461 gaaatgtgcg cgcagcagag ttacgaccaa cttcttgcag catcaacaga aaactggcgt
    17521 caatggtggc agaaacgtcg tatcacggta aacggcggcg atgcgcacga tcagcaagcg
    17581 ttagattatg cgctttatca tctgcgcatc atgacgcctg ctcacgacga gcgcagcagt
    17641 attgcggcaa aaggcttaac cggagaaggc tacaaaggcc acgttttctg ggatacagaa
    17701 gtatttttgc taccgtttca tctgtttagc gatccgacgg ttgcccgaag tttactgcgt
    17761 tatcgctggc acaacttgcc aggcgcgcag gagaaagcgc gacgcaacgg ctggcagggc
    17821 gcgctatttc cgtgggaaag cgcgcgcagc ggcgaagaag agacgccgga atttgccgcc
    17881 attaacattc gcaccgggct gcggcaaaaa gtggcttcgg cgcaggcgga acatcatctg
    17941 gtcgccgata tcgcctgggc ggttattcaa tactggcaga ccacagggga tgaaagtttc
    18001 attgctcatg aaggcatggc gctacttctg gagacggcaa agttctggat tagccgcgcg
    18061 gtgagagtta acgatcgtct ggaaattcat gatgttattg ggccagacga atataccgaa
    18121 catgtcaata ataacgcctt caccagctat atggcgtatt acaacgtcca gcaggcgctg
    18181 aacatcgctc gtcaatttgg ctgtagcgac gatgcgttta tccatcgcgc cgaaatgttc
    18241 cttaaagaac tgcggctgcc agaaattcag cccgacggcg ttttgccgca ggatgattcg
    18301 tttatggcta agccggcgat taacctggcg aaatacaaag cggcggcggg aaagcaaacc
    18361 attctgctgg attattcacg cgcagaagtg aacgagatgc agatcctcaa acaagctgat
    18421 gtggtgatgc tcaattacat gctgccggag cagttctcag cggcatcgtg tcttgccaat
    18481 ctgcaatttt atgaaccgcg cactattcac gactcgtcat taagtaaagc aatccacggc
    18541 attgttgccg cacgctgtgg cctgctgacc caaagttatc agttctggcg cgaggggact
    18601 gaaatcgatc ttggtgctga tccgcatagt tgtgatgatg gtatccacgc tgccgcaact
    18661 ggcgctatct ggctgggggc gattcagggt tttgccgggt tgagcgtgcg tgacggtgaa
    18721 ttgcatctca atccggcgtt acctgagcag tggcaacagt tgtctttccc tctgttctgg
    18781 cagggctgcg aattacaggt cacgctcgac gcgcagcgta ttgcgattcg aacttctgcg
    18841 cccgtttcac tgcgtttgaa cggccagctt atatccgtgg ctgaagaatc tgttttctgt
    18901 ttgggcgatt ttattttgcc cttcaatggg accgctacca cgcatcggga ggatgaatga
    18961 aactgcaagg agtagttttc gacctggatg gtgtaatcac cgataccgcg catctgcatt
    19021 tcaaggcgtg gcagcagatt gctgctgaaa ttggcatcag cattgatgcg cagtttaacg
    19081 aatccctaaa agggatcagc cgcgatgagt ctctgcggcg cattctgcaa cacggggaca
    19141 aagagggcga ctttaacccg caggagcggg cgcaactggc gtatcgcaaa aatctgctct
    19201 acgtccactc actgcgcgaa ctgacggtta atgctgtgtt gcccggcatt cgctctttgc
    19261 tggcagatct ctgcgcacag cagatcccta tagggctggc ttctgtctcc ctgaatgcgc
    19321 cgacgatttt agcggcgttg gagctacgtg agtttttcac cttctgcgcg gacgcttccc
    19381 aacttaaaaa ctcgaaaccg gacccggaaa tctttctcgc tgcctgtgcc gggctgggcg
    19441 tgccgccgca ggcatgtatc ggcattgaag atgcgcaggc gggcattgac gccattaacg
    19501 ccagtggtat gcgctcggtg gggatcggcg cgggcttaac cggggcgcaa ttactgttgc
    19561 cctcaacgga cttcctgacc tggtcgcggt tatcggcctt ctggcaaaac gtatagcaaa
    19621 ggaatcaaca tggctcagct ttcgttacaa catattcaaa aaatctacga taaccaggtg
    19681 catgtggtga aggacttcaa cctggaaatt gccgataaag agttcatcgt atttgtcggt
    19741 ccgtcaggct gcggtaaatc gactacgctg cgcatgattg ccgggcttga ggagatcagc
    19801 ggcggcgatc tgttgatcga cggcaaacga atgaatgacg ttccggctaa agagcgcaat
    19861 atcgcgatgg tgttccagaa ctatgcgctg tatccgcata tgacggtcta cgacaacatg
    19921 gcgtttggcc tgaagatgca aaaaatcgcc aaagaggtga ttgatgagcg cgttaactgg
    19981 gctgcgcaaa ttctcggcct gcgtgagtac ctgaaacgca agccgggcgc gctttccggc
    20041 gggcaacgcc agcgtgtggc gctcgggcgg gcgattgtac gcgaagcggg cgtgttttta
    20101 atggatgaac cgctctctaa ccttgatgcc aagctgcgcg tgcaaatgcg cgcagagatc
    20161 agcaagctgc accagaagct gaataccacc atgatctacg tgacccacga tcagaccgaa
    20221 gcgatgacca tggcgacgcg gattgtgatt atgaaggatg ggattgttca gcaagttggt
    20281 gcgccgaaaa cggtttataa ccaaccagcg aatatgtttg ttgccggatt tattggatca
    20341 ccggcgatga attttattcg tggcacgatc gatggcgata aattcgttac ggaaacgctt
    20401 aaattaacca ttcccgaaga gaaattagcg gttctgaaaa cacagggaag tttgcataag
    20461 cccatcgtga tgggaatacg tccggaagat attcatccgg acgcgcaaga ggaaaataac
    20521 atttccgcca aaattagcgt ggcagaatta accggtgcgg aatttatgct ctacaccacg
    20581 gttggggggc acgagttagt ggtccgtgct ggtgcgttaa atgattatca tgcaggagaa
    20641 aatatcacta ttcattttga tatgacgaaa tgtcatttct ttgatgcaga aacggaaata
    20701 gcaattcgct aaatacaggg ggaaggcatt cccccagaat aatacaagga acaataatga
    20761 aaaagttatt accctgtacc gcactggtga tgtgtgcggg aatggcctgc gcacaggccg
    20821 aggaaaagaa cgactggcac tttaatatcg gcgcgatgta cgaaatagaa aacgtcgagg
    20881 gttatggcga agatatggat gggctggcgg agccttcagt ctattttaat gccgccaacg
    20941 ggccgtggag aattgctctg gcctattatc aggaagggcc ggtagattat agcgcgggta
    21001 aacgtggaac gtggtttgat cgcccggagc tggaggtgca ttatcagttc ctcgaaagcg
    21061 atgatttcag tttcggcctg accggcggtt tccgtaatta tggttatcac tacgttgatg
    21121 aaccgggtaa agatacggcg aatatgcagc gctggaaaat cgcgccagac tgggatgtga
    21181 aactgactga cgatttacgt ttcaacggtt ggttgtcgat gtataaattt gccaacgatc
    21241 tgaacacgac cggttacgct gatacccgtg tcgaaacgga aacaggtctg caatatacct
    21301 tcaacgaaac ggttgccttg cgagtgaact attatctcga gcgcggcttc aatatggacg
    21361 acagccgcaa taacggtgag ttttccacgc aagaaattcg cgcctatttg ccgctgacgc
    21421 tcggcaacca ctcggtgacg ccgtatacgc gcattgggct ggatcgctgg agtaactggg
    21481 actggcagga tgatattgaa cgtgaaggcc atgattttaa ccgtgttggt ttattttacg
    21541 gttatgattt ccagaatggt ctttccgttt cgctggaata cgcgtttgag tggcaggatc
    21601 acgacgaagg agacagtgat aaattccatt atgcaggtgt cggcgtaaat tactcgttct
    21661 gataatgggc taaattgccg gatgcggcgc gagcgccaaa tccggcatac atttatttca
    21721 acgacatatg cgtggcaacg gtaatattct gtggtgacgg ttttccagaa attaagcgga
    21781 ataataactc gcagctttgt tgacctaact cctgcgtcgg aacatcgatg ccgcccggtg
    21841 caggcgttaa tataaacgac agcgtttcat tactataacc caccaccgct aactgatgcg
    21901 gaatagcaat gtttttctct gccgctgcac gataaatgct cattaatttc aggctgtcag
    21961 tggcaaacac tgcctcaggc aacggtgact gacttaataa ttgccgtgct gcttgcagtg
    22021 ctgtttcatg ggtataaccg ccatcaacaa tccattcatc acgcactgca atattatgat
    22081 cttccagact ctgcttataa ccatttaccc gatccactga aacatggaca tcaagcggtg
    22141 catgcagaca ggcaatattt tgatgtccac tttcaattaa cgcatcggtc aacgcaatgc
    22201 tgtcgccaaa attatcggta tcgacagaat aaacatgggc atattgacct tcaactttgc
    22261 caatcaccac caccggaata tcgtatttat cgagttgcgc aaaaaacgat tcatcagcag
    22321 gtgagcttag cataataatg cctttaatca ttttctgctt aattttactt tcacattttt
    22381 gcagatcttc tgccgggttg tgcgacgtct gcaatatcac gtcgaaacct tcttcttcag
    22441 ctttggcggt gatggcatgc aaaacttcag agaaaaacgg attacccgcc gtagttttgg
    22501 tcgaacgggt agaaatcacc ataatggcat caaaacccga agaggtcagc gcgcgggcca
    22561 gcttgtttgg ttgatactgt aattcttcaa tggcccgtaa cactttttcg cgcgcttccg
    22621 gggagatatt ggtttgctta ttcagcacgc gtgatacggt ggattttgat acgcctgcaa
    22681 ccctggcaat atcataaata gtaggggaca taggtcagga actccgtccg aacgtgaatg
    22741 ctgcacatct tatggagttc gcccgtcgat gacaacacgg gtagttgata atcaatggcc
    22801 tggcccccac attcatatcc ttatgaatga tttttttctg gtcttcagag cagcacagga
    22861 cagcaatgaa gcgacttaaa aatgaactta atgcgctggt gaatcggggt gtcgacagac
    22921 atctgcgcct cgctgtaacc ggacttagcc gcagcggcaa aacagcgttt atcaccgcga
    22981 tggttaatca gttgctcaat attcatgccg gagcacgttt gccgctgtta agtgcggtgc
    23041 gtgaagagcg cctgctgggc gtaaaacgca ttcctcagcg tgactttggc attccgcgtt
    23101 ttacctacga cgaagggttg gcgcagctgt atggcgatcc tcccgcctgg ccgacgccaa
    23161 cgcgcggcgt cagcgaaatt cgcctggcgc tacgctataa atcgaacgat tcgctgctgc
    23221 gccactttaa ggatacctcc acgctgtatc tggagattgt ggattacccc ggcgaatggt
    23281 tgctcgacct gccgatgctg gcgcaggact atttaagctg gtcgcgccag atgacgggct
    23341 tactcaatgg tcagcgcggc gaatggtcgg cgaaatggcg aatgatgtgc gaagggctgg
    23401 acccgctagc gcctgccgac gaaaaccggc tggcagacat tgccgccgcg tggaccgatt
    23461 atctccacca ctgtaaacag caggggctgc actttattca gccagggcgc tttgtcttgc
    23521 cgggagatat ggcaggtgcg cccgcgctgc aattcttccc gtggccggat gtcgatgcct
    23581 ggggcgagtc caaactggcg caggccgata agcacaccaa tgccggaatg ctgcgcgagc
    23641 ggtttaatta ttactgcgag aaggtggtga aggggttcta taagaatcat tttctgcgct
    23701 ttgaccgcca gattgtgctg gtggactgcc tgcaacctct caacagtggg ccacaggcat
    23761 ttaatgatat gcgtctggcg ctgacgcaac tgatgcaaag ttttcactac gggcagcgta
    23821 ccctgttccg gcgtttgttt tcgccggtta tcgataagct attgtttgct gccactaaag
    23881 cggaccatgt gaccatcgat cagcacgcta atatggtttc attactgcaa caactgattc
    23941 aggatgcctg gcaaaatgcg gcgttcgaag ggatcagcat ggactgcctg gggctggcgt
    24001 ctgttcaggc gaccaccagc ggcattattg atgttaacgg tgagaaaatc ccggcgctgc
    24061 gcggtaatcg acttagtgat ggcgcaccgc tcactgttta tcctggcgaa gtccccgcac
    24121 gtttgcctgg tcaggcgttc tgggataagc aaggcttcca gtttgaggcg tttcgtccgc
    24181 aggtgatgga tgtcgacaaa ccgctgccgc atattcgtct tgatgccgcg ctggaatttt
    24241 taataggaga taaattgcga tgaccgaacc gttaaaacca cgtattgatt tcgacggtcc
    24301 tctggaggtc gatcagaatc ctaaattcag ggcgcagcag acctttgacg aaaatcaggt
    24361 gcaaaatttt gccccggcca cgctcgacga agcgcaggaa gaagaggggc aagtcgaagc
    24421 ggtaatggac gcagcgttac gtccgaaacg cagcctgtgg cgcaaaatgg tgatgggcgg
    24481 gctggctctg tttggcgcaa gcgttgtcgg gcagggtata cagtggacaa tgaatgcctg
    24541 gcaaactcag gactgggtgg cgctgggtgg atgtgccgct ggggcattga ttatcggcgc
    24601 tggcgtaggt tctgtggtaa cagagtggcg gcgcttatgg cgcttgcgac agcgcgccca
    24661 tgaacgcgac gaagcgcgtg atttattgca tagccacgga accggcaaag gccgcgcatt
    24721 ttgcgaaaaa ctggcgcagc aggcgggtat tgatcagtcg catccggcgc tgcaacgctg
    24781 gtatgcctca atccatgaaa cgcaaaacga ccgtgaagtg gtcagcctgt atgcgcattt
    24841 ggtccagcca gttttagatg cccaggcgcg gcgcgaaatc agccgttcgg cagcggaatc
    24901 aacgttgatg attgcggtca gcccgctggc gctggtggat atggcattta tcgcctggcg
    24961 caatctgcgt ttgattaatc gcatcgccac gctgtatggc attgaactgg gatattacag
    25021 ccgtttgcgc ctgttcaagc tggtattgct gaatatcgct ttcgccggag ccagcgaatt
    25081 ggtgcgcgaa gtggggatgg actggatgtc gcaagatctc gctgctcgtt tgtctacccg
    25141 cgcagctcag gggattggtg ctgggcttct gacggcacga ctcgggatta aagctatgga
    25201 gctttgccgc ccgctgccgt ggattgacga tgacaaacct cgcctcgggg atttccgtcg
    25261 tcagcttatc ggtcaggtga aagaaacgct gcaaaaaggc aaaacgccca gcgaaaaata
    25321 atgcaatatc gggtgctgac cggatatctt tacgccgaag tgcccgtttt tccgtctttg
    25381 tgtcaatgat tgttgacaga aaccttcctg ctatccaaat agtgtcatat catcatatta
    25441 attgttcttt tttcaggtga aggttcccat gcgtctggaa gtcttttgtg aagaccgact
    25501 cggtctgacc cgcgaattac tcgatctact cgtgctaaga ggcattgatt tacgcggtat
    25561 tgagattgat cccattgggc gaatctacct caattttgct gaactggagt ttgagagttt
    25621 cagcagtctg atggccgaaa tacgccgtat tgcaggtgtt accgatgtgc gtactgtccc
    25681 gtggatgcct tccgaacgtg agcatctggc gttgagcgcg ttactggagg cgttgcctga
    25741 acctgtgctc tctgtcgata tgaaaagcaa agtggatatg gcgaacccgg cgagctgtca
    25801 gctttttggg caaaaattgg atcgcctgcg caaccatacc gccgcacaat tgattaacgg
    25861 ctttaatttt ttacgttggc tggaaagcga accgcaagat tcccataacg agcatgttgt
    25921 tattaatgga cagaacttcc tgatggagat tacgcctgtt tatcttcagg atgaaaatga
    25981 tcaacacgtc ctgacaggtg cggtggtgat gttgcgatca acgattcgta tgggccgtca
    26041 gttgcaaaat gtcgccgccc aggacgtcag cgccttcagt caaattgtcg ctgtcagccc
    26101 gaaaatgaag catgttgtcg aacaggcgca gaaactggcg atgctaagcg cgccgctgct
    26161 gattacgggt gacacaggta caggtaaaga tctttttgcc tacgcctgcc atcaggcaag
    26221 cccaagagcg agcaaacctt acctggcgct gaactgtgcg tctataccgg aagatgcggt
    26281 cgagagtgaa ctgtttggtc atgctccgga agggaagaaa ggattctttg agcaggcgaa
    26341 cggtggttcg gtgctgttgg atgaaatagg tgagatgtca ccacggatgc aggcgaaatt
    26401 actgcgtttc cttaatgatg gcactttccg tcgggttggc gaagaccatg aggtgcatgt
    26461 cgatgtgcgg gtgatttgcg ctacgcagaa gaatctggtc gaactggtgc aaaaaggcgt
    26521 gttccgtgaa gatctctatt atcgtctgaa cgtgttgacg ctcaatctgc cgccgctacg
    26581 cgactgcccg caggacatca tgccgttaac cgagctgttc gtcgcccgct ttgccgacga
    26641 gcagggcgtg ccgcgtccga aactggccgc tgacctgaat actgtactta cgcgttatgc
    26701 gtggccggga aatgtgcggc agttaaagaa cgctatctat cgcgcactga cacaactgga
    26761 cggttatgaa ctgcgtccac aggatatttt gttgccggat tatgacgccg caacggtagc
    26821 cgtgggcgaa gatgcgatgg aaggttcgct ggatgaaatc accagccgtt ttgaacgctc
    26881 ggtattaacc cagctttatc gcaattatcc cagcacgcgc aaactggcaa aacgtctcgg
    26941 cgtttcacat accgcgattg ccaataagtt gcgggaatat ggtctgagtc agaagaagaa
    27001 cgaagagtaa gcgcgaatat gcctgatggt gcaacaccat caggcatatt aacttatgct
    27061 ttcagtacag ccagagctgc ttcgtaatcc ggctcggtgg tgatttcatc caccagctga
    27121 ctgaaaatca cattgtcatt ttcatcaata accacaacgg cacgcgctgc cagacctttc
    27181 agtgggccat cagcaattgc cacaccgtag gcttgcagaa attcagcgtt acggaaagtg
    27241 gagagggtga taacgttgtt cagaccttct gcgccgcaga aacgagactg ggcgaacggc
    27301 agatcggcag aaatacacag cacaacggtg ttgtcgatct cggttgccag ttggttaaac
    27361 ttacgtactg atgcggcgca aacaccggta tcaatactcg ggaaaatgtt cagcactttg
    27421 cgtttacccg caaactgacc gagggtgacg tcagacagat cttttgccac gagagtaaaa
    27481 gtctgcgctt tgctacccgc ctgcgggatg gagttggcga ctgtaaccgg gttgccctgg
    27541 aaatgaacgg tttgtgacat gattatcttt cctgtttaca tatagttaac gtcacaccta
    27601 gtttatgcta actgtcaata gcacagcaaa cgctatttgc gcttaatccg cagaccaccg
    27661 cgacaacaag gagtaaagat gagaaccgtt aaggtattcg aggaagcctg gcccttacat
    27721 accccgtttg tgattgcccg gggaagtcgc agtgaagcgc gcgtggtggt ggttgaactg
    27781 gaagaagagg gtattaaagg caccggcgaa tgcacgccgt atccgcgtta tggagaaagt
    27841 gatgcctcgg taatggcgca aattatgagc gtcgtgccgc aattagagaa agggctgaca
    27901 cgggaggagt tgcaacaaat tctccctgcc ggcgcagcac gtaatgcgct ggattgtgca
    27961 ttgtgggatc tggccgcgcg aaaacagcag caatcgctgg ctgatttgat cggcataacg
    28021 cttcccgaga cagttatcac tgcacagacg gtagtcatcg gtacgcctga tcagatggcc
    28081 aatagtgcat caacactctg gcaggccggc gcgaaattac tgaaagtgaa gctggataac
    28141 catcttatca gtgagcggat ggtggcaatt cgcacagctg tgcccgatgc cacgctgatc
    28201 gttgatgcaa atgaatcctg gcgtgcagaa gggttggcgg cacgttgcca gctattggcg
    28261 gatttaggcg ttgcgatgct tgaacaaccg cttcctgcgc aggacgatgc ggcactggag
    28321 aattttattc atccgttgcc gatttgtgct gatgaaagtt gtcatactcg tagcaatctg
    28381 aaggcgctga aagggcgcta tgagatggtt aacattaagc tcgataaaac cggggggctg
    28441 acggaagcgc tggcgctggc gactgaagcg cgtgcacaag gtttcaatct gatgctgggc
    28501 tgcatgttgt gtacctctcg tgccattagc gccgctttac cgctggtgcc gcaggtcagt
    28561 ttcgccgatc ttgacggacc gacctggctg gcggtagatg tggaacaggc gcttcagttc
    28621 acgacgggcg aattgcatct ttaggatgcc agcgcagcaa gtttgccatc gcaaagagat
    28681 atttttcgct ggcttcgtcg gaggagatgg gcggaaactc tgcggtgatg caatgcaagt
    28741 tgagatctgc acaccaactg ccgaaagagc caggcgtttc ataaccgacg ctggttacca
    28801 gaggcaattc aaacgcctgg gccagccatt cgcctaattc gctgtgtctg ggatcttcaa
    28861 tacaagccag tggatcgtgg aatgagacca cccaggcagg ttgaatgcgg tgtataagct
    28921 ggcacagcgc ctgggtttca ggttcggagc caggtttgtc acctgtcagt aatacgacat
    28981 cacgttcttc agcggcgctg ttccagcgat aaaccgtttc accttccttc cagttcgccg
    29041 ccggaaagtt tctgtttaaa tccacaccat tagcattggc ccgtaacccc agctggcagc
    29101 cgtcaggatt cacacacagc accacatgat ggcggcgcaa agaaggtgtc aaagtccgca
    29161 gagcacagga gagcgtgacg accgaagagt tttcatcacc gtgagtgcca gccagaatca
    29221 aaccactttc gcgactggct gcaggggccg gaaaccagat taacggcgcg cccaataatg
    29281 aacgtccgta atgttctgtt ccgggcggaa atgcgccgcg ttcggcgcgt gggcgggtta
    29341 cggtcatgat cgtctctggt tcaggaagta ttacaggcag tgttgtgcaa attttgtgga
    29401 ttatcaaatc cttttcttca acgctatgtc atcgatcttg tatcactttt ttgccggaag
    29461 ttgtttgtat tttcattgat gtgcagatat tagaattgtc gtaatgttat atacatggcg
    29521 tattttattc ttactggttc tacacatgtt aatcgcatct gattgatcag ttcaccttaa
    29581 taagtttaat aaatcgtact gcacatagta cagtacgatg gtggctatat aattgattta
    29641 catgaagaat ggcttgtcgc catcagtccc gggttgattg atgcctatat tctcaccaat
    29701 gtgtttctca ggtttatcga tgatatctgt aattaatgct gcgacactct tacgtgatac
    29761 gatcgtacct ttgaatggtt cattgcgtga ggtaagctca taatcaataa tatcttcatc
    29821 cgtcagccag gcagggcgaa gaattgtata ttccagtcca gaagcctcaa ttgcatccgc
    29881 agccctgcgg aatggtttta ggggctcacc aataactgca ttattccatt cgccaaattt
    29941 tcctggaact tcatcataaa tgccgaggga aagaacaaag atcagacgtt taacatcaca
    30001 ggctttcatc gcagcgatca ctgaatttgc ctgaatatct aaatcttccc ccgtaaggtt
    30061 ggcatacaca atatcctgtc cctgcatggc ctgcttcagt gctgcatgat tcagtacatc
    30121 ccccataata atctggctat tagttggata gggtttgtgt atttttgctg gctgacgagc
    30181 aaataaagtt tgtttaatgg tttgtttatc tgccagttga ttaatcacat ggcgggctat
    30241 ctgaccgcca gcaccaagaa tgagtacatt tttcataata tttctcatga aacttatcac
    30301 agatgggtgt tgaaaaaagg aataactttt tccagcgcct cctgaactgc ttccggtttg
    30361 tcatacagtt cataatgtga gaaatcgggt aatgaaacta actgacgatc ctgcgaaacc
    30421 atcgcccggc catagacttc cagtccatca cgataagcac cgaaggcacc gactttttca
    30481 ccaactacta ccatcaccgg ctgagtcagt aacacttcag taaatgcgaa ggcatcccag
    30541 gccagcgttt tctgtgcatg tgagaacaac atttttgtcg cacctcccgg ttgttgcccg
    30601 cgtggtgttt tatagtagtc agttgcttca taaacatcgc gttcggtcag tccatgggct
    30661 tttgctgcat ccaggctggc gggaagtaat tcgttgatct gtaattctcc accacgagcc
    30721 tctttggttc gttgcgacgc cattgcattc aatgcgccaa tcggatcgta attgctgaaa
    30781 ccttcccgaa aaagacgccc gatattaaca cccgtaatac tcactacagc tttaattcgt
    30841 ttttctgtca gcgcggcatt aatggcatag ccaccccccc cacacacacc cagcacgcca
    30901 atgcgttcag catcaacata tggcagagtt actgcataat cgataacacg gctaatatcc
    30961 tctacgcgct gtgtcggatc ttctatccaa cgaggctccc caccagattc tccctggaaa
    31021 ctggcgtcat aagcgagaac gacatagcct ttttcagcca atgccttgcc atagatattg
    31081 ccagacgtct gctccttaca actgccaaaa ggatggacgc tgataatcgc cggatactgt
    31141 tttgactcat caaaatttgg tggaaactga atattggcag caatatgcca ggccatatca
    31201 ctattgcgga tttgaatatg tttcattaca gagtttcctc aaagaacgga gctaatactg
    31261 aaattgcttc agccacatag gctttgccgt cataaagatc catatggttt gcgccttcaa
    31321 caatgtgata gcgtttatcc tggcttgatg cccgatcgta caggtcgtca ctcatccatt
    31381 tgctccctgc ctggctgccc gccacaatct gcgtcggctg agtcaggtac acttccgcca
    31441 tatggtaagc atcataggta ataatctggt taaggctgcg caaagtagcg taacccggtg
    31501 ctgttggata ctgcgcgcga ggggtgtggt aatactccca ggcctgacgc agttcttcgt
    31561 tcggcgcatc ggactccttc attggtgcca gtggcataat ggcgtattct ccgctgctaa
    31621 tatcgctggt tctggcgttt gaacccgctt caacgtatgg cagggcatca atagatttca
    31681 cattgttttc ccaaccatta cggaacatcg aaccaatatt gaccgcacta acggtaccga
    31741 tggccttgat gcggcgatcc tgaattgcag cattggctgt atatcctgca ccggcacaaa
    31801 ttcccatcgc accaattcgg gtattgtcga cataagaaag cgttgtcagg taatcaatca
    31861 cggcactgat gtcttcagta cgaatgtatg ggttttctaa ctgacgcggc tcgccgccac
    31921 tttcaccctg ataagatgcg tcataagcaa tagtgacaaa tcccttttcc gccagttttt
    31981 cggcataggt cccggccgtt tgttctttaa cgcccccacc tgggtgagat accacaattg
    32041 ccggatattg acgggtttca tcaaattttg gaggaaaata gatcactgca gacaaagaga
    32101 tggttggatt attgctgtta gtgaagctga ctttattatt catcattccg ttcctcatgg
    32161 agttgttggc tcgttttaac ggttggtgat atcactatag atattgatca ttaagttgat
    32221 tagacccaaa atcatgatta gacctatcat ttaaatgatt aatagattgt atggagatac
    32281 tgaatgaagc gtgaagaaat cgctgatctg atggcgtttg tcgtcgttgc agaggagcgt
    32341 agcttcactc gcgcagcagc ccgcctgagc atggcgcagt cagctttaag ccagatagtg
    32401 cgccgtatag aagaacgatt gggattgcgg cttctgacgc gaactacgcg cagcgttgta
    32461 cccactgaag cgggcgagca tcttttgtct gttcttggcc ctatgttgca tgacatagat
    32521 tctgccctga catccctgag cgatctgcag aaccgcccat ccgggacaat acgtattact
    32581 actgtagaac atgcagcaaa aacgatattg ttaccagcaa tgcgcacatt cctgaaatcg
    32641 tatcctgaaa ttgatattca gctcaccatt gattatggtt tgaccgatgt cgtttctgaa
    32701 cgttttgatg caggcgtccg tctgggtggg gagatggata aagatatgat cgccattcga
    32761 atcgggccag atataccaat ggctattgtt ggctcaccgg attatttttc tcgccgaagt
    32821 gttccaacgt cagtgtcaca attaatagat catcaggcaa ttaatttgta tcttcccaca
    32881 tcgggtacag caaatcgctg gagattaata cgcggtggac gtgaagttcg tgttcgcatg
    32941 gaaggtcagc ttttactgaa tacgatagac ctgatcattg atgctgcaat tgatgggcat
    33001 ggattggcgt atctacctta tgatcaggtt gagcgggcta ttaaagaaaa aaaactgata
    33061 cgtgttttgg ataaattcac accagattta cgcggttatc acctgtacta tccacaccgt
    33121 cgacatgctg gctcggcatt ctcattattt atagataggc tgaagt
//
LOCUS       DAZKFU020000021        32583 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZKFU020000021 DAZKFU020000000
VERSION     DAZKFU020000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.021"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            207..1580
                     /gene="aroP"
                     /locus_tag="ACHOQQ_RS04015"
                     /old_locus_tag="pgaptmp_000803"
     CDS             207..1580
                     /gene="aroP"
                     /locus_tag="ACHOQQ_RS04015"
                     /old_locus_tag="pgaptmp_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGU4469098.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1623..2477)
                     /gene="ampE"
                     /locus_tag="ACHOQQ_RS04020"
                     /old_locus_tag="pgaptmp_000804"
     CDS             complement(1623..2477)
                     /gene="ampE"
                     /locus_tag="ACHOQQ_RS04020"
                     /old_locus_tag="pgaptmp_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGU4469099.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2474..3025)
                     /gene="ampD"
                     /locus_tag="ACHOQQ_RS04025"
                     /old_locus_tag="pgaptmp_000805"
     CDS             complement(2474..3025)
                     /gene="ampD"
                     /locus_tag="ACHOQQ_RS04025"
                     /old_locus_tag="pgaptmp_000805"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGU4469100.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDISNNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3113..4006
                     /gene="nadC"
                     /locus_tag="ACHOQQ_RS04030"
                     /old_locus_tag="pgaptmp_000806"
     CDS             3113..4006
                     /gene="nadC"
                     /locus_tag="ACHOQQ_RS04030"
                     /old_locus_tag="pgaptmp_000806"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGU4469101.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4209..4649
                     /gene="ppdD"
                     /locus_tag="ACHOQQ_RS04035"
                     /old_locus_tag="pgaptmp_000807"
     CDS             4209..4649
                     /gene="ppdD"
                     /locus_tag="ACHOQQ_RS04035"
                     /old_locus_tag="pgaptmp_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGU4469102.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTIRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4659..6044
                     /gene="gspE"
                     /locus_tag="ACHOQQ_RS04040"
                     /old_locus_tag="pgaptmp_000808"
     CDS             4659..6044
                     /gene="gspE"
                     /locus_tag="ACHOQQ_RS04040"
                     /old_locus_tag="pgaptmp_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGU4469103.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6034..7236
                     /gene="hofC"
                     /locus_tag="ACHOQQ_RS04045"
                     /old_locus_tag="pgaptmp_000809"
     CDS             6034..7236
                     /gene="hofC"
                     /locus_tag="ACHOQQ_RS04045"
                     /old_locus_tag="pgaptmp_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HGU4469104.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRFPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7271..8314)
                     /gene="guaC"
                     /locus_tag="ACHOQQ_RS04050"
                     /old_locus_tag="pgaptmp_000810"
     CDS             complement(7271..8314)
                     /gene="guaC"
                     /locus_tag="ACHOQQ_RS04050"
                     /old_locus_tag="pgaptmp_000810"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGU4469105.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8470..8514)
                     /locus_tag="ACHOQQ_RS04055"
                     /old_locus_tag="pgaptmp_000811"
     CDS             complement(8470..8514)
                     /locus_tag="ACHOQQ_RS04055"
                     /old_locus_tag="pgaptmp_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469106.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8539..9159
                     /gene="coaE"
                     /locus_tag="ACHOQQ_RS04060"
                     /old_locus_tag="pgaptmp_000812"
     CDS             8539..9159
                     /gene="coaE"
                     /locus_tag="ACHOQQ_RS04060"
                     /old_locus_tag="pgaptmp_000812"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGU4469107.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9159..9902
                     /gene="zapD"
                     /locus_tag="ACHOQQ_RS04065"
                     /old_locus_tag="pgaptmp_000813"
     CDS             9159..9902
                     /gene="zapD"
                     /locus_tag="ACHOQQ_RS04065"
                     /old_locus_tag="pgaptmp_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGU4469108.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9912..10109
                     /gene="yacG"
                     /locus_tag="ACHOQQ_RS04070"
                     /old_locus_tag="pgaptmp_000814"
     CDS             9912..10109
                     /gene="yacG"
                     /locus_tag="ACHOQQ_RS04070"
                     /old_locus_tag="pgaptmp_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGU4469109.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10325..10714)
                     /gene="mutT"
                     /locus_tag="ACHOQQ_RS04075"
                     /old_locus_tag="pgaptmp_000815"
     CDS             complement(10325..10714)
                     /gene="mutT"
                     /locus_tag="ACHOQQ_RS04075"
                     /old_locus_tag="pgaptmp_000815"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGU4469110.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10774..13479)
                     /gene="secA"
                     /locus_tag="ACHOQQ_RS04080"
                     /old_locus_tag="pgaptmp_000816"
     CDS             complement(10774..13479)
                     /gene="secA"
                     /locus_tag="ACHOQQ_RS04080"
                     /old_locus_tag="pgaptmp_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGU4469111.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13541..14053)
                     /gene="secM"
                     /locus_tag="ACHOQQ_RS04085"
                     /old_locus_tag="pgaptmp_000817"
     CDS             complement(13541..14053)
                     /gene="secM"
                     /locus_tag="ACHOQQ_RS04085"
                     /old_locus_tag="pgaptmp_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGU4469112.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14284..15201)
                     /gene="lpxC"
                     /locus_tag="ACHOQQ_RS04090"
                     /old_locus_tag="pgaptmp_000818"
     CDS             complement(14284..15201)
                     /gene="lpxC"
                     /locus_tag="ACHOQQ_RS04090"
                     /old_locus_tag="pgaptmp_000818"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGU4469113.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15302..16453)
                     /gene="ftsZ"
                     /locus_tag="ACHOQQ_RS04095"
                     /old_locus_tag="pgaptmp_000819"
     CDS             complement(15302..16453)
                     /gene="ftsZ"
                     /locus_tag="ACHOQQ_RS04095"
                     /old_locus_tag="pgaptmp_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU4469114.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16514..17776)
                     /gene="ftsA"
                     /locus_tag="ACHOQQ_RS04100"
                     /old_locus_tag="pgaptmp_000820"
     CDS             complement(16514..17776)
                     /gene="ftsA"
                     /locus_tag="ACHOQQ_RS04100"
                     /old_locus_tag="pgaptmp_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGU4469115.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17773..18603)
                     /gene="ftsQ"
                     /locus_tag="ACHOQQ_RS04105"
                     /old_locus_tag="pgaptmp_000821"
     CDS             complement(17773..18603)
                     /gene="ftsQ"
                     /locus_tag="ACHOQQ_RS04105"
                     /old_locus_tag="pgaptmp_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGU4469116.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18605..19525)
                     /gene="ddlB"
                     /locus_tag="ACHOQQ_RS04110"
                     /old_locus_tag="pgaptmp_000822"
     CDS             complement(18605..19525)
                     /gene="ddlB"
                     /locus_tag="ACHOQQ_RS04110"
                     /old_locus_tag="pgaptmp_000822"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4469117.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQADMSFSQLVVRILELAD"
     gene            complement(19518..20993)
                     /gene="murC"
                     /locus_tag="ACHOQQ_RS04115"
                     /old_locus_tag="pgaptmp_000823"
     CDS             complement(19518..20993)
                     /gene="murC"
                     /locus_tag="ACHOQQ_RS04115"
                     /old_locus_tag="pgaptmp_000823"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGU4469118.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAKVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21047..22114)
                     /gene="murG"
                     /locus_tag="ACHOQQ_RS04120"
                     /old_locus_tag="pgaptmp_000824"
     CDS             complement(21047..22114)
                     /gene="murG"
                     /locus_tag="ACHOQQ_RS04120"
                     /old_locus_tag="pgaptmp_000824"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGU4469119.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22111..23355)
                     /gene="ftsW"
                     /locus_tag="ACHOQQ_RS04125"
                     /old_locus_tag="pgaptmp_000825"
     CDS             complement(22111..23355)
                     /gene="ftsW"
                     /locus_tag="ACHOQQ_RS04125"
                     /old_locus_tag="pgaptmp_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGU4469120.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23355..24671)
                     /gene="murD"
                     /locus_tag="ACHOQQ_RS04130"
                     /old_locus_tag="pgaptmp_000826"
     CDS             complement(23355..24671)
                     /gene="murD"
                     /locus_tag="ACHOQQ_RS04130"
                     /old_locus_tag="pgaptmp_000826"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGU4469121.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADGRCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKISGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24674..25756)
                     /gene="mraY"
                     /locus_tag="ACHOQQ_RS04135"
                     /old_locus_tag="pgaptmp_000827"
     CDS             complement(24674..25756)
                     /gene="mraY"
                     /locus_tag="ACHOQQ_RS04135"
                     /old_locus_tag="pgaptmp_000827"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGU4469122.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25750..27108)
                     /gene="murF"
                     /locus_tag="ACHOQQ_RS04140"
                     /old_locus_tag="pgaptmp_000828"
     CDS             complement(25750..27108)
                     /gene="murF"
                     /locus_tag="ACHOQQ_RS04140"
                     /old_locus_tag="pgaptmp_000828"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGU4469123.1"
                     /translation="MISVTLSQLTDILNGELQGADITFDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27105..28592)
                     /gene="murE"
                     /locus_tag="ACHOQQ_RS04145"
                     /old_locus_tag="pgaptmp_000829"
     CDS             complement(27105..28592)
                     /gene="murE"
                     /locus_tag="ACHOQQ_RS04145"
                     /old_locus_tag="pgaptmp_000829"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGU4469124.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     NDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28579..30345)
                     /gene="ftsI"
                     /locus_tag="ACHOQQ_RS04150"
                     /old_locus_tag="pgaptmp_000830"
     CDS             complement(28579..30345)
                     /gene="ftsI"
                     /locus_tag="ACHOQQ_RS04150"
                     /old_locus_tag="pgaptmp_000830"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGU4469125.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30361..30726)
                     /gene="ftsL"
                     /locus_tag="ACHOQQ_RS04155"
                     /old_locus_tag="pgaptmp_000831"
     CDS             complement(30361..30726)
                     /gene="ftsL"
                     /locus_tag="ACHOQQ_RS04155"
                     /old_locus_tag="pgaptmp_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGU4469126.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30723..31664)
                     /gene="rsmH"
                     /locus_tag="ACHOQQ_RS04160"
                     /old_locus_tag="pgaptmp_000832"
     CDS             complement(30723..31664)
                     /gene="rsmH"
                     /locus_tag="ACHOQQ_RS04160"
                     /old_locus_tag="pgaptmp_000832"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGU4469127.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31666..32124)
                     /gene="mraZ"
                     /locus_tag="ACHOQQ_RS04165"
                     /old_locus_tag="pgaptmp_000833"
     CDS             complement(31666..32124)
                     /gene="mraZ"
                     /locus_tag="ACHOQQ_RS04165"
                     /old_locus_tag="pgaptmp_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGU4469128.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7747 a         9170 c         8028 g         7638 t
ORIGIN      
        1 aacggtttct ttttaagcaa ctcatcttca accatgcata aagcgggtgc attcgctgcc
       61 gcataccatt attcttgatc tgacggaagt ctttttgtaa caattcaaac ttctttgatg
      121 taaacaaatt aatacaacaa acggaattgc aaacttacac acgcatcact gcgtagatca
      181 aaaaaacaat caccgcacga ggtttcatga tggaaggtca acagcacggc gagcagctaa
      241 agcgcggcct taaaaaccgc catattcagc ttatcgcgct gggtggcgcg atagggaccg
      301 ggttattcct gggtagcgcc tccgtaatac agtccgcagg gccagggatt atcctgggtt
      361 acgccattgc tggttttatc gcctttctga tcatgcgtca gctgggtgaa atggtggtcg
      421 aagaacctgt cgcaggctcc tttagccact ttgcttataa atactggggc agctttgctg
      481 gcttcgcttc tggctggaac tactgggtac tgtacgtttt agttgccatg gcagagctga
      541 ctgctgtggg taaatacatt cagttctggt atccggaaat cccaacctgg gtttctgccg
      601 ccgtgttctt tgtggtgatt aacgccatca acctgaccaa cgtaaaagtg tttggtgaga
      661 tggagttctg gtttgccatt atcaaagtta ttgcggtagt agcgatgatc atcttcggcg
      721 gctggctgct gttcagtggt aacggcggtc cgcaggcaag cgttagcaac ctgtgggatc
      781 agggtggttt cctgccgcac ggcttcaccg ggctggtgat gatgatggcg attatcatgt
      841 tctcgttcgg tggtctggaa ctggtgggga tcaccgcagc agaagctgat aacccggagc
      901 aaagtatccc gaaagcgact aaccaggtta tctaccgcat cctgattttc tatattggtt
      961 cgttagccgt tctgctctca ctgatgccgt ggacccgcgt taccgccgat accagtccgt
     1021 ttgtgctgat cttccacgag ttaggcgata cctttgtggc gaatgcgctg aacatcgtgg
     1081 tgctgactgc ggcgctctcc gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt
     1141 ttggtctggc acaacagggt aatgcgccaa aagcgctggc gtctgtcgat aaacgcggcg
     1201 taccggttaa caccattctg gtgtctgcac tggtaacggc attgtgcgta ctgattaact
     1261 atcttgctcc ggaatccgca tttggcctgt taatggcgct ggtggtatct gcactggtga
     1321 tcaactgggc gatgattagc ctggcgcata tgaaattccg tcgcgccaag caggaacaag
     1381 gcgtggtaac tcgcttccct gctctgcttt atccgctggg taactggatc tgcctgctgt
     1441 ttatggcggc ggtactggtg attatgctga tgaccccagg aatggcgatt tcggtatacc
     1501 tgatcccggt atggctgatc gtgttaggta tcggctatct gtttaaagag aaaaccgcca
     1561 aagccgtaaa agcgcattaa tctctctacg ccctcacctg tacacggtga gggcaataat
     1621 ctttacacca acgccccgta aatcgtcagt agcgcaatca ccaccacgac cacgaacgag
     1681 gttttcttcg ccattgaaac cgctgccttc ggcgtctcca ccttatcgac atgcggttca
     1741 cgcgccagag agaactgcgc cagacgcgtt aacacctgat actgcgaagt atggaaatca
     1801 cccagcgaag caaaccaggc cggtaacgct ttctcaccat gaccgatcaa ggcatatacc
     1861 acacccgcaa gacgaactgg cacccaatcc agtacatgaa gcacggcatc aatgccggac
     1921 tgtaaacgat gatgcggcgt ctggtatcgc gccagccagt attgccatgc acgcaagaac
     1981 gcatacccca tcagcgtaac gggtccccag gttcccccca caatcagcca gaacagcggt
     2041 gcaagataaa aacgaaagtt aatccacagc aatgcatttt gcagctcacg caaatactca
     2101 cgttcgtcgc agcctgccgg gacgccgtga atcatggtga gttcgccagc catcgtggca
     2161 cgggcatggc tatcattacg tgaagcagct gtcagataag catgataatg aagacgaact
     2221 ttacctgcgc caatacacag caaaccaatc agcagccaca ccagcagcgt gggaacgttg
     2281 aacaatacgc cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc aataatggtc
     2341 atgcctaacg tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga
     2401 tgatcaagct gccagtgctc gcccaactta aacaggcgct cgaaaattaa caccagtaag
     2461 gttgtaaata gcgtcatgtt gtctccttgc tgaccagcgc acgaaaccgt gcccaatcaa
     2521 atgcaggacc gggatcggtt ttccgatccg gcgcaatatc acaatggccc gtcatgttat
     2581 tagagatatc cggatagcaa tcaatcagtg cccgcgtaac cgccgcaagc tgttgatact
     2641 gcgcatcggt atacgccagc gtatcggtgc cttcaagctc aatcccaata gaaaaatcat
     2701 tgcagcgttc gcgcccctga tactgagaga ctcccgcatg ccatgcacgt ttatcgaaag
     2761 gaacatactg gactatttca ccatcacggc gaatcaaaca gtgagcggag acgcgcaaat
     2821 gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat agttccagtg aataatgcgt
     2881 cgatccacgg accgccaaac tcgcctggcg gcaggctaat attgtgcacc accagcaggg
     2941 tgggtgtttc gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa
     3001 ccagccaccc ctgttctaac aacatgcagg atctccttat atgtggtgct aatacccggt
     3061 tcagagtagc atgtttctac cttatgattc gttagctatc tggagtttta acatgccgcc
     3121 tcgccgctat aaccctgaca cccgacgtga cgagctgctg gaacgcatta atctcgatat
     3181 ccccggcgcg gtggcccagg cgctgcggga agatttaggc ggaacagtcg atgccaacaa
     3241 tgacattacg gcaaaacttt taccggaaaa ctctcgctct catgccacgg tgatcacccg
     3301 cgagaatggc gtattttgcg gcaaacgctg ggttgaagag gtgtttattc aactggcagg
     3361 cgacgatgtc accataatct ggcatgtgga tgacggcgat gtcatcaacg ccaatcaacc
     3421 cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg ggcgaacgca ctgcgcttaa
     3481 ttttgtgcaa accctttcag gagttgccag taaggtacgc cactatgtcg aattactgga
     3541 aggcaccaac acgcagttgt tggatacgcg caaaacctta cccggcctgc gttcagcgct
     3601 gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt ctggggcttt ctgatgcctt
     3661 cctgatcaaa gaaaaccata ttattgcctc cggctcagtg cgccaggcgg tcgaaaaagc
     3721 gtcctggctg cacccggatg cgccagtaga agtcgaagta gagaatctgg aagaacttga
     3781 tgaagccctg aaagcaggag ccgatatcat catgctggat aacttcgaaa cagaacagat
     3841 gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac
     3901 tgacaaaaca ctgcgcgaat ttgccgaaac gggcgtggac tttatctccg tcggtgcgct
     3961 aactaagcac gtacaggcac tcgacctttc aatgcgtttt cgctagttgc tgacagattt
     4021 atccgctcca tagtgggcgg ataatcaccc cttccccaac tttcttcgta acgcctcgca
     4081 aatttcttgt ctttcgctgc aacggaataa aaaacctcgg aagctgcttt cccgtttctt
     4141 tttttgcccc tcgcttgtgc tgatggcgtt tgccaaagta gcgccaaccc aatcaaggag
     4201 cgaaacagat ggacaagcaa cgcggtttta cacttatcga actgatggtg gttattggca
     4261 tcattgccat tttaagcgcc attggtattc ccgcttatca aaactacctg cgcaaagccg
     4321 cactcaccga catgctacaa acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc
     4381 tggaacatgg tggattagat acctgcgacg gtggcagcaa tggcattccc tcgcctacca
     4441 ccatccgcta tgtttcagcc atgagtgtgg caaagggcgt ggtgtcgctg accgggcaag
     4501 aaagtctcaa tgggctaagc gtcgtcatga cgccaggttg ggataacgca aacggcgtca
     4561 ccggctgggc gcgcaactgc aatattcaaa gtgacagcgc attgcagcaa gcctgcgaag
     4621 atgtcttccg ctttgatgac gccaactaag gagcggcaat gaatattcca cagctcacgg
     4681 ccctgtgtct gcgttatcag ggagtcttgc tggatgccag cgaagaagtg gttcatgttg
     4741 cggtggtcga tgccccctca catgagttgc tggacgcatt gcatttcgct accaccaaac
     4801 gtattgagat cacctgctgg acgcgccaac aaatggaagg tcacgccagt cgcacacaac
     4861 agacattgcc cgtagctgtt caggagaagc atcagcccaa agcagagttg ctgactcgaa
     4921 cgttacaatc tgcgctggaa caacgcgcgt ctgatattca tatcgaacca gcggacaatg
     4981 cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc tttaccggat gtttcaccgg
     5041 atgccggagt cgcattaacc gccagattaa aagtgctggg aaacctggat attgcggaac
     5101 atcgcctgcc gcaggacggg caattcactg tcgaactggc aggaaacgcc gtctcatttc
     5161 gtattgcgac cttaccatgt cggggtggtg aaaaggtggt attaaggttg ttacagcagg
     5221 tgagccaggc actggatgtc aacacgctgg gaatgcagcc gttacaactg gcggactttg
     5281 ctcatgcctt gcaacaacca cagggactgg tgctggtaac tggccctacc ggcagcggca
     5341 aaacggtcac gctttatagt gccctgcaaa cgctgaatac cgctgacatt aatatttgta
     5401 gcgtcgaaga tccggttgag atccccatag ccggactaaa ccagacgcaa atccatccgc
     5461 gtgccggact cacctttcag ggcgttttgc gtgcgttatt gcgccaggat cctgacgtca
     5521 tcatgatcgg agagatccgc gatggcgaaa cagcagagat cgctattaaa gccgcgcaaa
     5581 ccggtcacct ggtgttgtct accctacaca ccaattccac ctgcgaaacg ctggtacgtt
     5641 tacagcaaat gggagtcgcc cgctggatgc tctcatcagc gcttacgctg gtaatagccc
     5701 agcgtctggt acgtaaactt tgcccacatt gtcgccagca gcaaggggag cccatccata
     5761 ttccagacaa tgtatggccg tcgccgctgc cccactggca ggcacctggt tgtgtacatt
     5821 gctaccacgg tttttatggt cgcacggcct tatttgaagt tctgcccata acaccggtca
     5881 tacgtcagct tatttccgct aataccgacg ttgaatcgct ggaaactcac gcccgacagg
     5941 cgggtatgcg tacgcttttt gaaaacggct gcctcgccgt ggagcaaggc ttaactacct
     6001 ttgaagagtt aatccgcgta ctggggatgc cgcatggcga gtaagcaact ctggcgctgg
     6061 catggcataa ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga gagccgtact
     6121 ttactgctta tggcactaca gcaacagatg gttaccccac taagcctgaa gcgaatcgcc
     6181 atcaattctg cgcagtggcg gggagataaa agcgcggaag tcattcatca actggcgacg
     6241 ctactcaaag cagggttaac gctttctgaa gggctggctc tgctggcgga acagcatccc
     6301 agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca gggcattgct
     6361 ttttccaatg ccttattacc ctggtcagag gtatttccgc cgctctatca ggcgatgatc
     6421 cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc gcgtcagcaa
     6481 aaagcccagc gtcagttgac cgacaaagtg aaatcagcgt tgcgttatcc catcatcatt
     6541 ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgttctgcc ggagtttgcc
     6601 gctatatata agaccttcaa caccccacta ccggcactaa cgcaggggat catgacgctg
     6661 gcagacttta gtggcgaatg gagctggctg ctggtgttgt tcggcattat gctgacgata
     6721 gccaataagt tgctgatgcg ccgaccgacc tggcttatcg cgcggcagaa attgctgtta
     6781 cgcttcccga ttatgggttc actgatgcgg ggacaaaaac tcacgcagat ctttacgatt
     6841 ctggcgctga ctcaaagtgc aggcattact tttttacagg gcgtagagag cgtcagagaa
     6901 acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa tccagcacga tatcagtaac
     6961 ggtcatccca tctggctggc gctaaaaaat gccggtgagt ttagcccgct ctgtttgcaa
     7021 ttagtgagaa cgggagaggc ctccggctcg ctggatctca tgttagacaa cctcgcccat
     7081 catcatcggg ataacacaat ggcgctggcg gataacctag cagccttact ggaaccggcg
     7141 ttgctgatca taacgggagg aattatcggt acgctggtgg tggcgatgta tctgccaatt
     7201 ttccatttag gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc tccacaccag
     7261 cgttggaaga ttacaggttg ttgaagatgc ggttttcttg ttcctgcaca cggataaatg
     7321 tggtgcgctt ggtcagttct ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca
     7381 ggccgcccaa aatatcacgc gcggtatttt caaccgggcc tcgcagcggc agcttaacgg
     7441 ttttaccttc tgctgcgcga tattccgcaa cgccgccaac gtgacgtttc atcgcagact
     7501 cggagctcat gccgtagaac agcataaatt tctcgccgtt ctcctcaacg atgcgaccgc
     7561 cgctctcttc gtggcccgcc agcatgccgc caagcatgac gaaatcggca ccgccgccaa
     7621 aagctttcgc gacatcgccc ggcgtggtgc aaccaccatc gctgacgatc attccgccca
     7681 gaccgtgcgc agcatcggca cattcgatta ccgcagaaag ttgcggataa ccgacgcctg
     7741 ttttgacacg agttgtacaa acagaacctg ggccaatgcc aactttaacg atatcggcac
     7801 cggagaggat aagctcctca cacatttcac cagtcactac gttgccagca caaatggttt
     7861 tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg gaataaccat
     7921 tcgccacatc aatacaaacg aagtttaatg ccgggttcag gtcgagaatc tgtttagttt
     7981 tttcgaaatc cgcatcagac gtaccggtag aaaccatcac atgtttcagc acatcagcgg
     8041 aagaattgtt gataaacgct tgccactctt cgacagaata gtgtttatgc acagcagtca
     8101 aaatatcaaa agaagccagc gcagaggcca tagaaaatgt gcctacggtg tccatatttg
     8161 cggcgataat cggcacgccg gaccagctct gacctgaatg tttgaaggtg aattgacgtt
     8221 ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg agaacgtctt
     8281 taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg ttaatggcta
     8341 taagggtaag agcgcaactc cagtgacgct atcatacgca ctaatcaatg cggcgcaaga
     8401 ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa atagtgagcg
     8461 aacgtttcat caatttcgct ttcgtgcgct catttttaac gttttgataa tcatgatgaa
     8521 ttcccgggaa tataaattat gaggtatata gttgccttaa cgggaggcat tggcagtggc
     8581 aagagtaccg ttgccaatgc gtttgctgat ctcggaatta acgtcattga tgccgatatt
     8641 attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc tgatcacttt
     8701 ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc
     8761 ttcgccaacc cggaagagaa aaactggctt aacgacctgc tgcatccgct gattcagcaa
     8821 gagacgcaac accagatcca gcaagcaacc tccccctatg tactgtgggt tgtgccattg
     8881 ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga tgtcagccca
     8941 gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa ctcgcgagca tgtcgaacaa
     9001 atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga cgtcattgat
     9061 aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc acactatttg
     9121 cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag gtcctttttg
     9181 aacatccact aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg attcagcaac
     9241 tcaccgttaa tttacccatc gttgaccacg ctggcgcgct gcatttcttc cgtaatgtca
     9301 gtgaattact ggatgttttc gagcgcggcg aagtccgcac tgagctgttg aaagaacttg
     9361 accggcagca acgtaaactc cagacctgga ttggcgtgcc tggcgtggac cagagccgta
     9421 ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt attaatctcc gcgccgcgta
     9481 tcgggcaatt tctgcgtgaa gatcgcttga ttgctctggt gcgtcagcga ctgagcatcc
     9541 caggcggctg ttgcagcttt gatttaccaa cattgcacat ttggctgcat ctaccccagg
     9601 cgcagcgcga cagccaggta gaaacctgga ttgccagcct gaacccgctc acccaggcac
     9661 ttaccatggt gctggattta attcgccagt cggccccctt ccgtaaacaa accagcctga
     9721 atggttttta tcaggataac ggtggcgatg ccgacttgct gcgcctgaat ctgtcgctcg
     9781 attcacagct ttatccgcaa atttccggtc ataagagccg ttttgccatt cgttttatgc
     9841 cgctggacag tgaaaacgga caggtaccgg aacgtctgga tttcgaactg gcctgttgct
     9901 aaggagtaaa aatgtcagaa actattacgg tgaattgccc aacctgcggg aaaacggtgg
     9961 tgtggggtga aatcagcccg tttcggccat tttgctccaa acgttgtcag ctgatcgacc
    10021 tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt tccgaaagcg
    10081 atgactggag cgaagaacca aagcagtgac atttgtctga tgccgcacgt aggcctgata
    10141 agacgcggac agcgtcgcat caggcatcgt gtgcaaatgt cggatgcggc gtgaacgcct
    10201 tatccgacct acaaaaaaaa atttatcaat gagttagaaa tccctttagg cctgataaga
    10261 cgcgccagcg tcgcatcagg catcgtgtgc aaatgtcgga tgcggcgaaa acgccttatc
    10321 tgatctacag acgtttaagc ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg
    10381 cattaagacc gactagcgac atccactcac cgggttgccc ttctttaccc cacggctccc
    10441 cttcccagct ttcgaccagc cagaaccaca gagttatatg cctgtccggg aattcatatt
    10501 ccagtttttc aaatagcgaa aaatgttgtg gggtaatccc gacttcttcc tgaagttcac
    10561 gcaccaccgc ctgttccggc gtttcaccca tttcaatttt accgccggga aactccagtt
    10621 tattcgccat gtgcgcatct gctgcgcgac gcgttataaa gatttcattg ttctcgttgc
    10681 gaataatacc taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca
    10741 gaatcctgcg ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt
    10801 gtatttttta ccggaaccgc acgggcaagg atcgttacgt cctactttgc gttcaccggt
    10861 ttgcgccgcc agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat
    10921 ttgcgctaaa cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cctcttcagg
    10981 catacgtacc tgaactttgc tcagcgtact gataacttca tatttcaacg actccagcat
    11041 cgctgcaaac atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata
    11101 gccacgcagg tggataccct gacgcagata gtccatcgct gccaggtgct ctttccacag
    11161 ggagtcaagc gtttgcagca tgacgccttt ctcgaagtga cgcatcatct cagcaccaac
    11221 cacttcttct ttacgctgat acacttcgat ggactgcgcc agaatgcgct cacgcagcgt
    11281 ctcttcatgc agttcgggtt ctttatccag ccactcagca attggtaaat cgaggtcgaa
    11341 atcgttcttc agacgttcct gcagccccgg aatatcccac atttcttcca gcgactgtgg
    11401 cggaatgtag gcatcaatgg tggctttgaa cacatcttca cgaatgctgt tgatggtttc
    11461 gctcacatcg ctgacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc
    11521 gttagccacg tcatcatatt ccagcagttg cttacgaatg tcgaagttac gactttcaac
    11581 tttacgctgg gcgttggcaa tcgctttagt cacccacggg tgttcaatgg cttcgcctgg
    11641 cttcataccc agtttacgca tcattccaga tactcggtcg gaagcaaaaa tacgcatcag
    11701 cgcatcttcc atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg
    11761 accgcgcaac tggttatcga tacgacggga ttcgtgacgc tcggtaccga taatatgcag
    11821 gccacctgct gccagtaccg catcgtgacg tacctgccag tcggctttaa ttttttcaat
    11881 ttgctctgcg gtcggatttt ccagcgcggc aacttctgcc tgccagctac caccgagcac
    11941 aatatctgta ccacgacccg ccatgttggt cgcgatagtc accgcagccg gataacctgc
    12001 ctgagcaaca atcgccgctt cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat
    12061 accggctttg gtcagttcgt ttgacaccag ctccgatttt tcgatggaga tagtacccac
    12121 cagcaccggc tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt
    12181 ttccgcttca gtcatgtaga ccaggtcagg cagatcttta cgaatcattg gacggttggt
    12241 cggaacaacg acggtatcca gtttgtagat ggagctaaat tcgaaagctt cggtatcagc
    12301 agtaccggtc atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga
    12361 agccagcgtt tggttttcgt tctggatctg cacaccttct ttcgcttcca cagcctggtg
    12421 cagaccatca gaccagcgac ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat
    12481 aacttcacca tctttaacga tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag
    12541 cgccgccgtt acgtgatgca tcagcatgat gttggccgga gagtacagag actccccttc
    12601 atccatgatg ccctctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag
    12661 gttcacctgg cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga
    12721 gtcttctttt tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt tatacatttc
    12781 cgagctgtct tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat
    12841 ggagtccact tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt cttcagggct
    12901 gaacgccatg ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat
    12961 gtcagctgcg taagcttcgc gctttgccgg tgctggcatg cccggcaggt tgataccgac
    13021 agtcaggcca aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta
    13081 gtcgttgacg gtaactacat gcacgccttt accggttagt gcgttcagat aagcaggcag
    13141 cgttgcggtc agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag
    13201 aaccataccg ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc
    13261 ctcacgtacc acggcgaaag cttccgggat cagattttcc agcacttcgc ctttttccag
    13321 acgtgcacga aactctgcgg ttttcccttt cagttcttcg tcggagagtt tttccatctc
    13381 cggttccatg gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt
    13441 acgactaccg aaaactttag ttaataattt gattagcata ataaaatctc aaacgccccg
    13501 cgttgcggag ttaataaaat gaagtaaagg tttattgttg ttagctgagg cgttgagggc
    13561 cagcacggat gccttgcgcc tggcttatcc agacgggcgt gctgaatttt gcttgtgggg
    13621 taaaatgcgc ataatcaatg cgataaccct tttcagacgg cgtgccttcc tgggtcagca
    13681 gcgcgctgag cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag
    13741 caacgggcag cgtttgcggt gccattgcga aggaaagatg acggattacc gtgcgaatgg
    13801 catgttgatg ccagtaatca acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca
    13861 gcaaggccaa ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt
    13921 ttgcgggcgc gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcaa
    13981 ccatccctaa taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca
    14041 gtattccact cacgttattg ccatcccgat cgtattcaaa cgtatttagc gcacaaatct
    14101 tatcattaaa gcctgaagtc cacagcacga atgaagataa gttgtccgta aaagtgctta
    14161 agaaaacagc gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaacgactgg
    14221 ttcacctggc cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg
    14281 tcgttatgcc agtacagctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg
    14341 gaaggtcaca tattcccagg cttcctgttt cgccaggaca gcctgcagca gtttgttatt
    14401 cagtgcatga ccggatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa
    14461 caagtcaccg atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc
    14521 gtcttcgttc agtacgcgat aatcgtcaac aacgatggca caatcgaagc tgccgcccag
    14581 gcacaaacca cgggactgca gatattcgat atcacgcatg aaaccgaacg tacgcgcacg
    14641 gctgatctgg cgcataaacg catcagcgga gaagttcatc gcatagcgct ggttgctgga
    14701 atcaatagcc ggatggttaa aatcgatggt gaaatccagc gaaaaaccat tgtacggctt
    14761 aaattcagcc cacttatcgc catcttcgac acgaacagtc tctttgatgc gaacaaattt
    14821 cttggcgcag ttcaactcgt cgataccggc gtcaagcagc aggtatacaa acggagcggc
    14881 gctgccgtcc atgatcggga tttccggcgc gttaacttcg ataacaatgt tatcgatgcc
    14941 caggcccgcg agagcagcat tgaggtgctc tacggttgaa atccgtacat catgctcgtt
    15001 gaccagacac gtacagagca tggtatcacg cacagatttg gcatcggccg ggaaatctac
    15061 cggtggattc aagtcggtgc gacgatagat gaccccggtg ttggccggcg cagggcgtaa
    15121 cgtcagggtg actttcttgc cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt
    15181 aagtgtcctt tgtttgatca tcgtattatc tcgccaaatt acctatccaa ccgaagtgta
    15241 ctatacattc ggcgggccag tttagcacaa agagcctcga aacccaaatt ccagtcaatt
    15301 cttaatcagc ttgcttacgc aggaatgctg ggatatccag ataatccggc tctttcgcag
    15361 tttgcggcgc attgtcattc acgactttag caaccggctt ctgctcctgg gtcagcggag
    15421 ccatcccgtg ctgctggtag cgatccatca ctggctgctg aacctgctta ttggtcacca
    15481 gagtgatttc aggacgtttg tccatgccga tacctgtcgc aacaacggtt acgcgcagct
    15541 cgtcattcat atccgggtca agagaagtac cgataaccac agtcgcgttg tcggaagcaa
    15601 atgcacggat ggtgttacct accgtttcga actcatccag acgcaggtcg aagcccgccg
    15661 tgatgttaac cagtacgccg cgcgcgccag acaggtcgat atcttccagc agcggagaag
    15721 agatagccat ttcagcagct tcttccgcac ggtcttcacc gctcgccacg ccagaaccca
    15781 tcattgcgta gcccatttca gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca
    15841 aacccggacg agtaatcagt tcggcgatac cttgcacagc gcctttgagt acatcgttcg
    15901 ctgcgccaaa cgcatccagc agggagatac cgcggcccag aactttcagc agtttgtcgt
    15961 tcgggatagt gatcagagag tccacatgct tggacagttc agtgatcccc tgctccgcga
    16021 atgccatacg cttcttgcct tcaaagttga agggcttagt gacgacagca acggtcagga
    16081 tacccaaatc ttttgccact tcagcgacga ctggtgctgc acctgtaccg gtaccaccac
    16141 ccatacccgc agcaataaag accatgtctg caccttccag cgccgcacgc agtgcatcgc
    16201 gatcctcatc agccgcattg cggccaactt ctggattagc gccagcgccc agtcctttgg
    16261 tgataccgct accgatttga atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat
    16321 cggtatttac cgcgaagaat tcaacacctt caatgcgctc gcgcaccatg tgttcaacag
    16381 cattaccgcc gccgccgccg acgccgatga ctttaatcac cgcgtcattg gtaagttcca
    16441 ttggttcaaa catagtttct ctccgatttg tgcctgtcgc ctgaggccgt aatcattgtc
    16501 ggcctcataa aaattaaaac tcttttcgca gccaactatt gagtcgcttg atccacgagc
    16561 caactgatgc tgtaacacgt ttttctactt cagcttcacc gttaagatgt gactctttcc
    16621 catagtgaag caatcccacc gccgttgaat aatacggctc ctgagcataa tccgttaaac
    16681 cggtaatgtt cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg
    16741 ctgcaagacc ttcgatctgc gctgcgccac cagttaatac aatgcctgcc gccaggtgat
    16801 gtttaacccc ttgttggcga agcttttcct gcaactgcaa tatctcttcg ttgaccaggt
    16861 tgagcagctc ggtatagcgc ggctcgatca cctctgccag ggtctgacgt tgcagactcc
    16921 gcggcggacg accgcctacg ctcggcactt ccacgctctc atcttttcca acgatggaac
    16981 ccagcgcaca accgtggcga actttaatcg cttcggcgtc gcttggcggc gtgccaaagg
    17041 cgtaagcgat atcactggtc acgacattgc cagcataagg aattacctta gtgtggcgca
    17101 atgccccacc ggtataaacg gcgatatcca ttgtaccacc accgatatcg acgacgcaga
    17161 cacccagttc acgttcatct tccgtcaata ccgaataact tgatgccaga ccggcaaata
    17221 tcagttggtc aactttcagc ccacaacgtt caaccgcttt gacgatgttt ttcgccatat
    17281 cgttgtggca tgtgatcagg tgcacttttg cctgcatccg cacgcccgaa agtcctaccg
    17341 gattcttgat cccttcctga tagtcaatcg catactcttg cgggatcaca tgcagcacac
    17401 gatgctcatc gcgcacacgc accgatttcg cggtatggac gacgttttcc acatcttctt
    17461 gcgtcacttc ttcttcagaa ataggcacca taccaatttc attctggcag ctgatgtgct
    17521 taccagaaag cgccagatat accgaagaga tctgacaatc tgccatcaat tctgcctggt
    17581 caatggcgcg ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg cctttatcca
    17641 taccacgcga cgggcagctg cccacgccaa tgatattgac cataccgtcg ggcagaactt
    17701 cccctactaa agcggcaacc ttcgcggtac caatctccag tcctactacc agttttctgt
    17761 ccgtcgcctt gatcattgtt gttctgcctg tgcctgattt tgttgctgag tagattcctc
    17821 tggcggcaag ggcgcccagc ctactgccgc tccagagtca taacgcaaat caacgtagct
    17881 aatccgtttg ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg
    17941 agccaaacgt ttcatcgtat cgccccggcc aagattgagc ttaatatcgt tattcagcgt
    18001 caactgccag gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc
    18061 cagcatctgc cccatttcgc gatagccctg caacacttca ttggcgctgc cttccgggcc
    18121 atacagcatt ggaagcacct gcttgctggt gcgatctggc ggcacgctga aggtatttcc
    18181 ttccgcgtct accatgtgtt gatcattcca ccgcgcaatc ggcacatatt caaccagatg
    18241 aatcttcaat tcatcaggcc actgctttct gacgctcacc tgcttaatcc acggcaggcg
    18301 ttgttctatt tgcgtctgga tgatgttgac atcctgggtc ataaaggtac ccggctcacc
    18361 caatgccagg atcgactgcc ggatatcgtc attacgcgtg taatggcgtt caccggtcaa
    18421 caccagcttt gagagcggca ggcgttgcgc atcttccatc cagcccaaca cgacccagcc
    18481 gctcaccaac actgtcgtta aaacggtcag caggaaaagg atccccgcca gacgcgttcc
    18541 attattgcgg cgagaagaaa cctcttcttc gctgtttcgc gtgttcagag cagcctgcga
    18601 catattagtc cgccagttcc agaattcgta ctaccaactg cgagaagctc atatctgcct
    18661 gacgtgccgc catcggcacc aggctgtggc tggtcatacc cggtgaggta ttggcttcca
    18721 gcagataaaa ctgtccatca ctgtccagca taacgtcaat acgtccccat cctttgcaac
    18781 ctaacgtcgt ccatgctttc agcactaatg cctgcaaatt ggcctcttgt gacgcttcca
    18841 gacctgcggg gcagaaatac tgtgtctcat cagagagata cttcgcctca taatcataga
    18901 aggttccgga cggttgaata cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg
    18961 tgaactccgg cccacttagc catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca
    19021 atcttaatgc atcttgtaga gcattttctg ctactacttt tgacattccc acactggaac
    19081 cttcgcggct cggcttaacg ataaccggca aacccagagc agaaatttct gctaactgct
    19141 tatcgctaag gcctttttca aactctgcgc gggttaacgc tacccacggt gcgaccggta
    19201 aaccagcacc ttgccacagc agtttgctgc gtagtttatc cattgaaagc gcagatgcca
    19261 tcactccgct gccggtataa ggcaagccca tcagctcgag catcccctgc agcgtaccat
    19321 cttcaccgcc gcggccgtgt agcgcgataa acactttctg aaagcccatc gacttcagtt
    19381 gcgtcacgtc gacttctttc gggtcgacag gatatgcgtc aataccgcct tcacgcagcc
    19441 cggctaacac cgctgcgcca gaattcaaag aaacttcccg ctcagcggag gtcccgccca
    19501 acaggaccgc gattttatca gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg
    19561 atttcagcta aagaacgggc aatttttccg atattaccag ccccctgaac gagaatcagg
    19621 tcgttaccgg ttaataccgg tgccagcatc tcggctacct tcgccggatc cggcaccaga
    19681 atgggatcta ttttcccacg tccacgaatt gtgcgacaca gcgaacggct gtccgctccc
    19741 ggaattggcg cttcgccagc cggatacact tccagcatca acagggtatc aacctgcgtc
    19801 agcacattgg cgaaatcatc atacaggtcg cgcgtacggg taaaacggtg cggctgaaac
    19861 agcattacca ggtttttatc cggccagcct gcacgcgccg ctttaatagt ggcgtccact
    19921 tccgtcgggt ggtggccgta gtcatcgacc agcattgccg taccgctttt accattcact
    19981 ggctccagcg ggaattcacc aaggaaatca aaacggcgac cagtcccctg gaagctctcc
    20041 agcgcacgca aaatagcctc gtcgtcaatg ccctcttccg tagcaaccgc aaccgcagct
    20101 gcggcgttca gcgcgttgtg acgacctggt gcattcaggg tgacgcgcat cggctctttg
    20161 tcctggcgca gcagcgtaaa gtgcccctgc gggccaatct gctgataatc ttctacacgc
    20221 acgtcggcat cttcgctgaa gccgtaagtt gtggtctgac gtcccactcg cggtaacaat
    20281 tcgcggatca ccggatcatc aacacacatc accgcacgac cgtaaaacgg caggttgtgc
    20341 agaaaattaa taaaagtctg ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg
    20401 tcggcttcga tattggtgac aatcgccacc atcggttgca gatgcaggaa cgatgcatca
    20461 ctctcatctg cttcggcaat caggtaccga ccatgcccca aacgcgcatg aacccccgcc
    20521 gcttttacca gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg
    20581 gagaccatcg cggtagttgt cgttttgccg tgcgttcctg caatggcgat gccatgacga
    20641 aaacgcatta actcagccag catttcggca cgacggatca ccggaatacg cgcttcatga
    20701 gctgcgacaa tttccgggtt atcggcagaa atagcgctgg aaacaacgac cacgctggca
    20761 tcacgtacgt tttccgggcg atggttgaaa taaatcgtcg ctcccagatt cattaactgc
    20821 tgcgtgaccg gatttggcgc taaatcggaa ccactgatct gataaccttc attggccaga
    20881 acttcggcaa taccgcccat accggcacca ccgatgccga caaaatgtat gtgccgaacg
    20941 cgacgcattt cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt cattctttac
    21001 gccattaact tcttaaattc atacgatgca aaaggcatcg ctacaattac gcccgggcag
    21061 cccggctcac ttcatttgcc actcgctcgg tggcatccgg aatggatgca gcgcgggcgc
    21121 gttctgccat ggttaataag gtttctcgcg accacccggc cagggtgttg gcgacagcat
    21181 ccacgctaag ctgtggctgc tcgataattt tggctgcgcc cgctttttcc agcggtagcg
    21241 cattccagta ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg
    21301 ccgcggcgat ttcactcacc gttaaagcac cggagcggca aacgacgaca tccgcccacg
    21361 catacgccgc cgccatatca tcaataaatt cagtcacttt atgctgcggt tgccccgctt
    21421 cggcatacgc ctgttcaacg gattgttgcg aacctttgcc gctctgatgc cagatagtga
    21481 ctgaatcacc cagtttcgca gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct
    21541 gggaaccacc cactaccagc acacgaaccg gaccttcacg tccagccaaa cgttgctgcg
    21601 gcaacggtag cgccaacaca tcggtacgca ccgggttacc caccacttcc gcattgggga
    21661 aagcacctgg aaacgcctgc atcactttgg tggcaatctt cgccagccat ttattggtta
    21721 agcccgcaat accgttttgt tcatgaagta caaccggaat gcctaacgac cacgcggcca
    21781 gaccaccagg acctgatacg tagccgccca taccgagcac cacgtcaggt ttgtacgctt
    21841 tcataatcgc ccgcgcctga cgccaggcat tgaagatacg cagcggcgca gctatcagtg
    21901 cttttatacc ttttccacgc agaccagaga tacgaatgaa atcaatttcg atgccatgtt
    21961 ttggcactaa gtccgcttcc atacggtcgg cagtccccag ccagcgaact tgccaaccct
    22021 gagccattag atggtgcgca accgccagtc ccgggaatac atgtccaccg gttccgcctg
    22081 ccatcaccat taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc
    22141 tttctccaga cgcgtttcat aatcaatacg caacagcatc atgatggctg tcgacataat
    22201 cagtaagctc gaaccaccgt aactgatcag cggcaatgtc agacctttgg tcggtaacat
    22261 ccccgccgcc gcgcctacgt taaccagcgc ctggaagcta aaccagatgc caatagaaca
    22321 ggcgagaaaa ccggaaaaac ggtggtcaat ttctaatgct ttacggccaa tcgacatcgc
    22381 gcgaaaagcg acgaagaata ccattaaaag tgccagcacc acaccgacat accccagttc
    22441 ttcgccgata atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact ccagtttttg
    22501 taccgagtta cctaaacctt gtccccacag ttcgccgcga ccaaacgcca tcagcgattg
    22561 cgttaactga tagccgctgc caaagggatc ttcccacggg ttccagaatg cggtaacacg
    22621 gcggatacgg tacggttcgg cgaggatcag caacacaacc gctgaaatgc ccataccgat
    22681 aatggcaatg aactgccaca atttcgctcc cgccaggaac aacatcgcca gcgtagtcac
    22741 aaacaacacc accaccgtac caaggtctgg ctgtgccagc agtaacactg ccaacaccag
    22801 aatcacgccc atcggtttca ggaagccgcg caggttatta cgtacttcgt cgcctttacg
    22861 caccagatag ttggcgatat agcaaaacag cgacagtttt gtcagctccg caggctggat
    22921 acgcagcaaa ccgagatcga tccaacgcga tgccccttta accgagctcc ccactaccag
    22981 cacgatcatc agcaggatga tagatccgag cagcatcgtg gcactgtagc gttgccagaa
    23041 ctccatcggc agacgcagcg taatgatcgc cagaataaac gccaaaatca gatagacacc
    23101 atcacgcttc gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg gcattgacgc
    23161 cgaggtcacc atgataaagc caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg
    23221 atcgtacatg atcaggctgt cggtatcttt ttcccgcgag cccatcaccc agccctttag
    23281 cgccgtggag atccagacca ggatactgaa tcctggcagg cgcggcattt tcaggcgagg
    23341 gagagataaa cgcatcaacc taactccttc gccagacggg caaactcatt gcctcgttgt
    23401 tcaaagttct tgaactgatc aaggctggca caggccgggg agagtagaac catatcgccc
    23461 ggctgaacac gcggagccag caagcgcatc gcctgttcca tagtttcggt ttgttctgcc
    23521 acttccgggc gtagcgccgc cagctgcacg ccgtcacgac cgaaacaata cagacgtacg
    23581 ttatcgccat tcaggtaacg cgccagtggg ctaaagtccg ccgatttacc atcgccaccc
    23641 agcaacaaat gcagcgtgcc gtctacgtgc aggccattca gcgccgcttc cgtactgccg
    23701 acgttggtcg ctttcgaatc gttaatccaa cgtacgccgt tatgctccag cacaacttca
    23761 aagcgatgcg gcagaccagt gaatgtggtt aacgctttca ggctgctggc tcgcggtaac
    23821 cctgcggcat ctgccagtgc cagcgccgcc agcgcattgg tgtagttatg ctgcccggaa
    23881 attttcattt ctttcacgtt cagcactttc tcgcctttca cccgcagcca ggtttcgccc
    23941 tgctgatgat tcaggtgata gtcacccatg ttgacgccaa agctgacgca gcgtccatcc
    24001 gcaccgcgaa tcggcattgt taaggcatca tcagcattaa ccacgcaaac tttcgcgttt
    24061 tcgtaaatgc gcagttttgc tgcacgatac tgttgtaaac caaacggata gcgatccata
    24121 tgatcttcag tcacgttcag aatggtcgct gctaccgcct gtaagctgga ggtggtttcc
    24181 agctggaagc tcgacagttc cagcacgtac agttcacact catcatccag tagcatcaac
    24241 gcaggcaggc caatattgcc acccacacca acgttaaccc ccgccgcttt cgccatttca
    24301 cccactagcg tggtgaccgt gcttttgccg ttagaaccgg taatcgccac aatcggtgct
    24361 tgtgcttcgc gacagaacag ctcgatatcg ccaacgattt cgattccggc atcagcggca
    24421 gcgcttaagg atggatgcgc cagtgcaata ccgggactgg cgacaatcag atctgccgcc
    24481 atcagccatt catcattcag actgcccgtg tggcgttcaa cggcttcggg taatttatcc
    24541 aggccaggcg gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa
    24601 aagtccacgc aggaaagccc ggtgaggccc aggccgataa tgacaacatt tttaccctgg
    24661 taatcagcca tgattaacgt accttcagcg ttgccagacc aatcaggacc agcatcagcg
    24721 aaataatcca gaaacgcaca atgacgcgcg gttccggcca gcctttcagt tcatagtggt
    24781 gatgaatcgg tgccatgcgg aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca
    24841 ggatgacaga aagcgtttct accacgaaca cgccccccat aatcaccagc aggaattcct
    24901 gacgtagcag tacggcgata atgcctaacg caccacctaa cgccagcgaa cctacatcgc
    24961 ccataaagac ctgcgccgga taggtgttaa accacaggaa gcccagtcct gccccgacta
    25021 tcgcggtaca gacaataacc agttccccgg cgtgtcgcag atacggtatg tgtaaatagc
    25081 tggcaaagtt catgttgccg gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata
    25141 cggtcggcat aattgccaga ccgtcaagac catcggtcag gtttaccgcg ttgccagtac
    25201 ccacaatgac gaagtaagcc agcaaaatgt agaacagccc cagttgcggc atcacatctt
    25261 taaagaatgg gaccaccagc tgcgttgcgg gcgtgtcttt gccaacaagg tacagcgcga
    25321 aggcgacacc cagcgcaatg accgacatcc agaaatactt ccaacgagcg atcaaccctt
    25381 tggtgtcttt acgcactact ttgcgataat catcaacaaa gccaataaca ccgtaaccta
    25441 ccagcaccac caacacgcac cagacgtacg gattggacgg gtaagcccac agcagtacgg
    25501 agatcacaat cgccgtcagg atcataatcc cgcccatggt cggcgtaccg cgcttgctga
    25561 agtgtgattc aggaccgtcg ttacgcacca cctgaccaaa ggaaagtttt tgcaaatgag
    25621 caatcatacg cgggcccatc cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg
    25681 cgcgaaacgt cagataggaa aagacgttaa agccggaata atatttgacc aaatgttcgg
    25741 ccagccaaac taacatgtcc cattctcctg taaagcgcgt actacctctt ccatggcggc
    25801 actacgtgaa cccttaacta aaatcgtaat tacctgttgc tcagcaatca gtgatttaag
    25861 acgcgtaatt aacgcagttt tatcagcaaa atgttcgcca acgccgctgg cggtgctgat
    25921 agcatggctt tgtttaccca cgcttaacac gcggtcaata ccagcagctt ttgccgcctc
    25981 gcccacctgt acatggcagg cttcgctttc agcgcccagt tccgccatat cgcccaccac
    26041 cagcacgcgg tagcccggca tttcagccag tacctggact gctgcggtca ttgaaccgac
    26101 attggcgttg taggagtcgt cgagcagcaa ctggttttct gccagttgga tggggaacag
    26161 acggcctgga acagctttca gatttgccag ccccgctttg atagcatcaa gcgttgcgcc
    26221 cacggacatg gagagcgcag cggctgccag cgcattcgca atattgtgac gccccggcaa
    26281 cggcagcaga acatccacgc taccggttgg ggtttgcagg gtaaattccg taccgtgcga
    26341 agtcacatgg atattggtgg cggtgaaatc gctgttggcg gcattaggtg agaaacgcca
    26401 cactttgcgt gagccaatta cgctctgcca gttcagccag tcgttgttgt cggcgttcat
    26461 aatggcgata ccgttttccg gcaggccgct aaagatttca cctttcgctt tcgcgacacc
    26521 cgcaagcgag ccaaaacctt ccagatgcgc cgctgccagg ttgttgacca gcgcagcttc
    26581 cgggcgagtc agactcacag tccaggcaat ttcgccctga tggttcgcgc caagttcaat
    26641 aactgcgtaa tcgtattccg gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt
    26701 gttgagattg cctgccgtat aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat
    26761 ctctttaacg gaggttttgc cggaggaccc cgtcagagca accacgcgcg ccggaacttg
    26821 ctggcgaacc catgcagcca gttcaccaaa cgccagacgc gtatccttga cgattaactg
    26881 cggcaggtcg atgtccagcg gacggctaac cagtagtgcg cccgcgccgc cagctttcgc
    26941 ctggtcggca aaatcatgag catcgaaacg ttcgcctttc agggcaacaa acaggcagcc
    27001 cggcgtcagt tttcgggtat cagtggttac agcatcaaag gtgatatctg caccttgcag
    27061 ttcaccgttg agaatgtcgg taagttggct aagggttacg ctaatcatgc aatcaccccc
    27121 agcagacgcg ccaccgtgac gcgatcggag tagtccagac gctgattgcc aacaatctgg
    27181 taatcttcat ggcctttgcc cgcgaccagc accacatcgt tctctttagc ctgcataacg
    27241 gcgcaagtca ccgcttcagc acggccttcc atcactttgg catgtccggc atctaacatt
    27301 cccgccagaa tatcgttgat gatggcacgc ggctcttcgg tacgcgggtt atcgtccgtc
    27361 accaccgcca catcagcaaa ctcttcggca attgcgccca tcagtggacg cttaccttta
    27421 tcgcgatcgc caccacagcc aaagacacac cacagcttgc ccgcacagtg cagacgcgcc
    27481 gcctgtaagg ctttttccag tgcatccggc gtatgcgcgt aatccaccac caccgtcggt
    27541 ttgcctggcg cagtgaacac ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt
    27601 ttcaacagat ccgccaatgg atagcccagt gccaacagcg tcgccagcgc gagcagcagg
    27661 ttgctgacgt taaaagcgcc catcagatgg ctctcaattt cgccatcgcc ccaacttgag
    27721 ctaaagcgaa tcgtcgcacc gctgtcgtga tagttcacgt cgttcgcttt taaccagcgt
    27781 ccgtgacagt tcggattgat atgatcttcc attgataccg caaccgcgtc cggcagtttt
    27841 gccagccagc ggcggcccac ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgc
    27901 tcagaataaa gcagccattt cgcggcttcg tagtgttcca tatcaccatg ataatcaagg
    27961 tgatcgcggc ttaagttggt aaagaccgac gccgcaaatt tcaatgccgc cacacggtgc
    28021 tgtaccagcc cgtgggagga aacttccatt gcgcaaaacg tcgcgccctg atccaccagc
    28081 cccgccagct cgtgctgaac atcgactgcc gaaccggtag tattttctgt cgggatcact
    28141 ttccccagca gaccgttacc aacggtaccc attaccgcgc tggtttcgcc aagcagttgg
    28201 ctccactgcg ccagcagctg ggtagtcgtg gttttgccgt tggtgcccgt tacgcctacg
    28261 agacgtaaat tgtcagaggg ttcatggtaa aagcggcccg ccagtgcaga taaacgctca
    28321 ttgagctggc tgagatagat gaccggtacg ccgtgcattt cacggatttc accgtcggtc
    28381 gcctcatctt tcgcctctgc aataatggca gccacacctt gcgctatcgc ctgcgggata
    28441 tatcgacgcc cgtccgcctg atgacctact acagctacaa agagatcgcc cgccgcagcc
    28501 acacggctgt cgagtgtcat ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga
    28561 gcaagaaggt cgcgcaaatt acgatctgcc acctgtcccc tcgccttgat taatcacaaa
    28621 ttcattttta tcgcccgttg tcagcgcatc cggctcgatg ttcatggtac gcaatacgcc
    28681 gcccatgatg gcaccaaaga ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg
    28741 cggatcgttg ataacaacaa ccagcgcgaa gcgcggctga ctcgcaggcg caacgcctgc
    28801 ggtataagca atatatttat tgatgtagcg accgtccggc ccgacctttt tcgcggtacc
    28861 ggttttaatg gcgatacgat agcctttaat cgccgccttc acgccgccgc cgcccggtag
    28921 cgccacgctt tccatcatat gcaccacagt gcggacaatg gattccggga agacacgttc
    28981 accgggaacc ggggggtcaa ctttggtaat cgacagcggg cgataaatgc cgtagctgcc
    29041 gatagttgcg tagactcgcg ctaactgtaa cggtgttacc attagcccgt agccgaaaga
    29101 gaaggtggcc ctctctatgt cagaccaccg ttgtttttga ggatataagc cactgcgttc
    29161 tccgaccaac cccaaattgg tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa
    29221 cgctgaggac ggcatcgcta acgccagctt ggaaacaccg acgttactcg acttctgtaa
    29281 taccccggtc agggttaatt cgctgtagcg tgccacgtct ttgatttcgt ggccgttaat
    29341 tcgataagga atggtattga gtaccgagtt ttcccgcacc acgccacgtt gcaacgcggt
    29401 catcaccacc atcggtttaa ccgttgagcc cggttcaaac acgtcggtga tggtacggtt
    29461 acgcatcgcc tcttttggcg tgccgctcag attatttggg ttgtatgatg ggctgttagc
    29521 catcgccagc acttcaccgg tgttgacatc caccagcacg gcgctaccgg actcagcctt
    29581 gttaaaggcc accgcgttat tcagttcgcg ataaaccagc gcctgcaggc gttcatcaat
    29641 actcagcgcc aggttgtgcg cagcctggct gtcagtagaa gaaatatctt caattacgcg
    29701 accatagcgg tctttacgca caatgcgctc acccggctgc ccggtaagcc atttatcgaa
    29761 actcttctcg acgccctcaa tcccctgact atcgacgtta gtaaagccga tgaggtgagc
    29821 agtcacttcg ccggatggat aataacggcg agattcttca cgcagatgaa tccccggcag
    29881 tttcagtttt ttgatgtagt ccgccatgtc agggtttacc tgacgcgcca gataaataaa
    29941 gcgccctttc gggttggcgt taatgcgggc tgaaagctga tccagcggaa tattgagcgc
    30001 gttagccagc gccttccagc ggtcaccgac gctgataccg ccagcgtcat gcacttcttt
    30061 cgggtcagcc caaatcgctt ttaccggcac gctcaccgct aacgggcgac cagaacggtc
    30121 agtaatcatg ccgcgggagg tggaaacttg ctgaacgcga agagaacgca tgtcgccctc
    30181 tttcaccagc atatccgggg agataacttg taaccacgct acgcgtccga gcagaaaagc
    30241 cagcgccagg agaatacagc cgcataacaa cgcaaaacgc caactgataa agttggcatg
    30301 ttcttcctga cgttttggtt tctgcgtttt cgccgctgct ttcatgcgtc gcgtttatcc
    30361 ttatttttgc actacgatat tttcttgtga cggatcaaca tgctgcattt gcagcttttc
    30421 cgtggcgatc ctttccaccc ggctatggtc gccgagcgca ttctcttcaa ggatcaggtt
    30481 gcgccattca atgtctaaag catctcgctc cagcaccagt tgttcgcgct gagcggtcag
    30541 taaacgggta tggtgcgccg tggttaccac ggtcaccgcc gtcaaaataa tgcaaatgaa
    30601 caggcagagt ggcagcttcc caaatcgcaa aagatcgtca ccgataacac caggcaatgc
    30661 atggcgctcg tggcttccca tcgatccttt aactttgctt agagcttctg tcactctgct
    30721 gatcatgcgt tcgtcctctc tgcaatacgc agaactgaac tacgggcacg aggattctca
    30781 gccacctctt cttcgcccgg cattaacttg cctagtgctc gcagctgacg gccacccagt
    30841 tttttgagct gctcttcagt catcggtaac cctgccggaa cttgcggacc gcggctgttt
    30901 tcacgcataa aacgtttcac aatacggtct tccagcgagt ggaagctgat gatcgaaagc
    30961 cgcccacccg gtgccagcac gttgagcgag ctttttagcg cctgctctat ctcctccagt
    31021 tcactgttta cccaaatgcg caccgcctgg aaggtacggg tcgcgggatg tttaaactta
    31081 tctttcaccg gcgttgcagc agccacgact tccgccagtt ctttggtgcg ggtcatcggc
    31141 tgttcgcggt tacgctcgac aatggcgcgg gcaatgcgtt tggcaaaacg ctcttcacca
    31201 taggttttca atacccaggc gatatcggct tcttctgcgg tttgtagcca ttcagcggct
    31261 gactgcccac gggttgggtc catacgcatg tccagcggac catcgcgcat aaaggaaaag
    31321 ccgcgttcag catcatcaag ttgcggtgaa gagacgccaa gatcgaggag aatgccgtcg
    31381 atcttgccga taagatcgcg ctcggcaacg tattcgccca gcgcggaaaa aggtccgtgg
    31441 atgatggaga agcgcggatc atcaatagtc ttcgcaacgg cgatagcctg cgggtcgcga
    31501 tcgatcgcca gcaaacgccc ctcttcgcca agctgcgaga ggatcagacg tgagtgacca
    31561 ccgcgaccaa aagtcccatc aatgtagatg ccatcaggac ggatattgag gccattaacg
    31621 gcttcatcca acagcaccgt agtatgttta tagttttcca tcattttata gagacaagtc
    31681 ctgcagtcgc tccgataagt ctccggtagc caactgctct gcgtcgatat cttccttgac
    31741 ctgttgatgc caggttgttt catcccacag ctcaaacttg ttgaactgtc caaccagcat
    31801 cacttctttt gtcagcccgg catgttgccg cagtactggc gcgattaaca atcgacctgc
    31861 gccatccatc tgacattcgc tggcatgacc taacagtagg cgctgcacac ggcgctcaac
    31921 cgggttcatg ctcgacagac gcgataattt ttgctcgata atttcccatt caggcagggg
    31981 gtaaagcagc aggcacgggt gatgaatgtc aatggtgcaa accatttgac cggcagcgtt
    32041 ctcaagcagc tgttcccgat aacgggtagg cactgataag cgccctttgc tgtcgagatt
    32101 gactaacgtt gctccccgga acatgccagc ctcacccctt attcccactt tgccccacaa
    32161 tttcccactg aaaggagttt acggagcgga ggaaaagctt gtcaagccag tcacaaggca
    32221 taccgaggta aaaaacccga tgtttacggc taatatcagc gttgtttatt ttacgtccaa
    32281 cgtgcgaagc aaattaacgt tatgaatagc tgtaagaaaa aaaccctcat cgtcatcacc
    32341 gttttaaaat gactcactat tttccacagc aaatataaag tgtcagtttg cgacgcgagc
    32401 ggcattttag gacatatctt ccccggttaa cagtccctgt tgcgtctgtg tagcgcttga
    32461 tgccagcggg tttgcgcaaa agcaaagaaa agtgcttgtt aaatcggcaa ttcatctctg
    32521 catgtaacaa aataatgcaa aaacgcgctg tcgatatcac ttaattattg tgaatttcag
    32581 ctg
//
LOCUS       DAZKFU020000022        32495 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZKFU020000022 DAZKFU020000000
VERSION     DAZKFU020000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.022"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(344..430)
                     /locus_tag="ACHOQQ_RS04170"
                     /old_locus_tag="pgaptmp_000834"
     tRNA            complement(344..430)
                     /locus_tag="ACHOQQ_RS04170"
                     /old_locus_tag="pgaptmp_000834"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(394..396),aa:Leu,seq:gag)
     gene            complement(445..777)
                     /gene="secG"
                     /locus_tag="ACHOQQ_RS04175"
                     /old_locus_tag="pgaptmp_000835"
     CDS             complement(445..777)
                     /gene="secG"
                     /locus_tag="ACHOQQ_RS04175"
                     /old_locus_tag="pgaptmp_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGU4469129.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1005..2342)
                     /gene="glmM"
                     /locus_tag="ACHOQQ_RS04180"
                     /old_locus_tag="pgaptmp_000836"
     CDS             complement(1005..2342)
                     /gene="glmM"
                     /locus_tag="ACHOQQ_RS04180"
                     /old_locus_tag="pgaptmp_000836"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGU4469130.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2335..3183)
                     /gene="folP"
                     /locus_tag="ACHOQQ_RS04185"
                     /old_locus_tag="pgaptmp_000837"
     CDS             complement(2335..3183)
                     /gene="folP"
                     /locus_tag="ACHOQQ_RS04185"
                     /old_locus_tag="pgaptmp_000837"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGU4469131.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3273..5207)
                     /gene="ftsH"
                     /locus_tag="ACHOQQ_RS04190"
                     /old_locus_tag="pgaptmp_000838"
     CDS             complement(3273..5207)
                     /gene="ftsH"
                     /locus_tag="ACHOQQ_RS04190"
                     /old_locus_tag="pgaptmp_000838"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGU4469132.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5307..5936)
                     /gene="rlmE"
                     /locus_tag="ACHOQQ_RS04195"
                     /old_locus_tag="pgaptmp_000839"
     CDS             complement(5307..5936)
                     /gene="rlmE"
                     /locus_tag="ACHOQQ_RS04195"
                     /old_locus_tag="pgaptmp_000839"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGU4469133.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6062..6355
                     /gene="yhbY"
                     /locus_tag="ACHOQQ_RS04200"
                     /old_locus_tag="pgaptmp_000840"
     CDS             6062..6355
                     /gene="yhbY"
                     /locus_tag="ACHOQQ_RS04200"
                     /old_locus_tag="pgaptmp_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGU4469134.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6511..6987)
                     /gene="greA"
                     /locus_tag="ACHOQQ_RS04205"
                     /old_locus_tag="pgaptmp_000841"
     CDS             complement(6511..6987)
                     /gene="greA"
                     /locus_tag="ACHOQQ_RS04205"
                     /old_locus_tag="pgaptmp_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGU4469135.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7235..8668
                     /gene="dacB"
                     /locus_tag="ACHOQQ_RS04210"
                     /old_locus_tag="pgaptmp_000842"
     CDS             7235..8668
                     /gene="dacB"
                     /locus_tag="ACHOQQ_RS04210"
                     /old_locus_tag="pgaptmp_000842"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4469136.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHTRFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8708..9880)
                     /gene="cgtA"
                     /locus_tag="ACHOQQ_RS04215"
                     /old_locus_tag="pgaptmp_000843"
     CDS             complement(8708..9880)
                     /gene="cgtA"
                     /locus_tag="ACHOQQ_RS04215"
                     /old_locus_tag="pgaptmp_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGU4469137.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9896..10861)
                     /gene="yhbE"
                     /locus_tag="ACHOQQ_RS04220"
                     /old_locus_tag="pgaptmp_000844"
     CDS             complement(9896..10861)
                     /gene="yhbE"
                     /locus_tag="ACHOQQ_RS04220"
                     /old_locus_tag="pgaptmp_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4469138.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(10988..11245)
                     /gene="rpmA"
                     /locus_tag="ACHOQQ_RS04225"
                     /old_locus_tag="pgaptmp_000845"
     CDS             complement(10988..11245)
                     /gene="rpmA"
                     /locus_tag="ACHOQQ_RS04225"
                     /old_locus_tag="pgaptmp_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGU4469139.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11266..11577)
                     /gene="rplU"
                     /locus_tag="ACHOQQ_RS04230"
                     /old_locus_tag="pgaptmp_000846"
     CDS             complement(11266..11577)
                     /gene="rplU"
                     /locus_tag="ACHOQQ_RS04230"
                     /old_locus_tag="pgaptmp_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGU4469140.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11836..12807
                     /gene="ispB"
                     /locus_tag="ACHOQQ_RS04235"
                     /old_locus_tag="pgaptmp_000847"
     CDS             11836..12807
                     /gene="ispB"
                     /locus_tag="ACHOQQ_RS04235"
                     /old_locus_tag="pgaptmp_000847"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGU4469141.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPKEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13035..13313
                     /gene="sfsB"
                     /locus_tag="ACHOQQ_RS04240"
                     /old_locus_tag="pgaptmp_000848"
     CDS             13035..13313
                     /gene="sfsB"
                     /locus_tag="ACHOQQ_RS04240"
                     /old_locus_tag="pgaptmp_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGU4469142.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13361..14620)
                     /gene="murA"
                     /locus_tag="ACHOQQ_RS04245"
                     /old_locus_tag="pgaptmp_000849"
     CDS             complement(13361..14620)
                     /gene="murA"
                     /locus_tag="ACHOQQ_RS04245"
                     /old_locus_tag="pgaptmp_000849"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGU4469143.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14675..14929)
                     /gene="ibaG"
                     /locus_tag="ACHOQQ_RS04250"
                     /old_locus_tag="pgaptmp_000850"
     CDS             complement(14675..14929)
                     /gene="ibaG"
                     /locus_tag="ACHOQQ_RS04250"
                     /old_locus_tag="pgaptmp_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGU4469144.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15089..15382)
                     /gene="mlaB"
                     /locus_tag="ACHOQQ_RS04255"
                     /old_locus_tag="pgaptmp_000851"
     CDS             complement(15089..15382)
                     /gene="mlaB"
                     /locus_tag="ACHOQQ_RS04255"
                     /old_locus_tag="pgaptmp_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGU4469145.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGVTCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15382..16017)
                     /gene="mlaC"
                     /locus_tag="ACHOQQ_RS04260"
                     /old_locus_tag="pgaptmp_000852"
     CDS             complement(15382..16017)
                     /gene="mlaC"
                     /locus_tag="ACHOQQ_RS04260"
                     /old_locus_tag="pgaptmp_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGU4469146.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16036..16587)
                     /gene="mlaD"
                     /locus_tag="ACHOQQ_RS04265"
                     /old_locus_tag="pgaptmp_000853"
     CDS             complement(16036..16587)
                     /gene="mlaD"
                     /locus_tag="ACHOQQ_RS04265"
                     /old_locus_tag="pgaptmp_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGU4469147.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16592..17374)
                     /gene="mlaE"
                     /locus_tag="ACHOQQ_RS04270"
                     /old_locus_tag="pgaptmp_000854"
     CDS             complement(16592..17374)
                     /gene="mlaE"
                     /locus_tag="ACHOQQ_RS04270"
                     /old_locus_tag="pgaptmp_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGU4469148.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17382..18191)
                     /gene="mlaF"
                     /locus_tag="ACHOQQ_RS04275"
                     /old_locus_tag="pgaptmp_000855"
     CDS             complement(17382..18191)
                     /gene="mlaF"
                     /locus_tag="ACHOQQ_RS04275"
                     /old_locus_tag="pgaptmp_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGU4469149.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAKALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18401..19378
                     /gene="yrbG"
                     /locus_tag="ACHOQQ_RS04280"
                     /old_locus_tag="pgaptmp_000856"
     CDS             18401..19378
                     /gene="yrbG"
                     /locus_tag="ACHOQQ_RS04280"
                     /old_locus_tag="pgaptmp_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGU4469150.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19392..20378
                     /gene="kdsD"
                     /locus_tag="ACHOQQ_RS04285"
                     /old_locus_tag="pgaptmp_000857"
     CDS             19392..20378
                     /gene="kdsD"
                     /locus_tag="ACHOQQ_RS04285"
                     /old_locus_tag="pgaptmp_000857"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGU4469151.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20399..20965
                     /gene="kdsC"
                     /locus_tag="ACHOQQ_RS04290"
                     /old_locus_tag="pgaptmp_000858"
     CDS             20399..20965
                     /gene="kdsC"
                     /locus_tag="ACHOQQ_RS04290"
                     /old_locus_tag="pgaptmp_000858"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGU4469152.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            20962..21537
                     /gene="lptC"
                     /locus_tag="ACHOQQ_RS04295"
                     /old_locus_tag="pgaptmp_000859"
     CDS             20962..21537
                     /gene="lptC"
                     /locus_tag="ACHOQQ_RS04295"
                     /old_locus_tag="pgaptmp_000859"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGU4469153.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21506..22063
                     /gene="lptA"
                     /locus_tag="ACHOQQ_RS04300"
                     /old_locus_tag="pgaptmp_000860"
     CDS             21506..22063
                     /gene="lptA"
                     /locus_tag="ACHOQQ_RS04300"
                     /old_locus_tag="pgaptmp_000860"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGU4469154.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22070..22795
                     /gene="lptB"
                     /locus_tag="ACHOQQ_RS04305"
                     /old_locus_tag="pgaptmp_000861"
     CDS             22070..22795
                     /gene="lptB"
                     /locus_tag="ACHOQQ_RS04305"
                     /old_locus_tag="pgaptmp_000861"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGU4469155.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22843..24276
                     /gene="rpoN"
                     /locus_tag="ACHOQQ_RS04310"
                     /old_locus_tag="pgaptmp_000862"
     CDS             22843..24276
                     /gene="rpoN"
                     /locus_tag="ACHOQQ_RS04310"
                     /old_locus_tag="pgaptmp_000862"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGU4469156.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24299..24586
                     /gene="hpf"
                     /locus_tag="ACHOQQ_RS04315"
                     /old_locus_tag="pgaptmp_000863"
     CDS             24299..24586
                     /gene="hpf"
                     /locus_tag="ACHOQQ_RS04315"
                     /old_locus_tag="pgaptmp_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGU4469157.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24704..25195
                     /gene="ptsN"
                     /locus_tag="ACHOQQ_RS04320"
                     /old_locus_tag="pgaptmp_000864"
     CDS             24704..25195
                     /gene="ptsN"
                     /locus_tag="ACHOQQ_RS04320"
                     /old_locus_tag="pgaptmp_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGU4469158.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25241..26095
                     /gene="rapZ"
                     /locus_tag="ACHOQQ_RS04325"
                     /old_locus_tag="pgaptmp_000865"
     CDS             25241..26095
                     /gene="rapZ"
                     /locus_tag="ACHOQQ_RS04325"
                     /old_locus_tag="pgaptmp_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGU4469159.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26092..26364
                     /gene="npr"
                     /locus_tag="ACHOQQ_RS04330"
                     /old_locus_tag="pgaptmp_000866"
     CDS             26092..26364
                     /gene="npr"
                     /locus_tag="ACHOQQ_RS04330"
                     /old_locus_tag="pgaptmp_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGU4469160.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26578..27210
                     /gene="yrbL"
                     /locus_tag="ACHOQQ_RS04335"
                     /old_locus_tag="pgaptmp_000867"
     CDS             26578..27210
                     /gene="yrbL"
                     /locus_tag="ACHOQQ_RS04335"
                     /old_locus_tag="pgaptmp_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGU4469161.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGERTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27207..27935)
                     /gene="mtgA"
                     /locus_tag="ACHOQQ_RS04340"
                     /old_locus_tag="pgaptmp_000868"
     CDS             complement(27207..27935)
                     /gene="mtgA"
                     /locus_tag="ACHOQQ_RS04340"
                     /old_locus_tag="pgaptmp_000868"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGU4469162.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPERWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(27932..28585)
                     /gene="elbB"
                     /locus_tag="ACHOQQ_RS04345"
                     /old_locus_tag="pgaptmp_000869"
     CDS             complement(27932..28585)
                     /gene="elbB"
                     /locus_tag="ACHOQQ_RS04345"
                     /old_locus_tag="pgaptmp_000869"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGU4469163.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28815..31151)
                     /gene="arcB"
                     /locus_tag="ACHOQQ_RS04350"
                     /old_locus_tag="pgaptmp_000870"
     CDS             complement(28815..31151)
                     /gene="arcB"
                     /locus_tag="ACHOQQ_RS04350"
                     /old_locus_tag="pgaptmp_000870"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGU4469164.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31247..32176)
                     /gene="yhcC"
                     /locus_tag="ACHOQQ_RS04355"
                     /old_locus_tag="pgaptmp_000871"
     CDS             complement(31247..32176)
                     /gene="yhcC"
                     /locus_tag="ACHOQQ_RS04355"
                     /old_locus_tag="pgaptmp_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGU4469165.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
BASE COUNT         8064 a         8832 c         7856 g         7743 t
ORIGIN      
        1 tttaaatgat gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
       61 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
      121 aagtaaacat atccgtattc gtaccggaac tggagttaac acaataaagt gccaattatg
      181 tcagcagtag ggaaaagttt ataacgaagg cgtaccggca ccatcgtttc aaggtgccag
      241 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc agaccaggac
      301 caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
      361 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
      421 ccacctcggc acggaaacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
      481 agctggctga gtttgttcgg ttttcgccgg cgcactcagg ttttcccatt cgctaccttt
      541 attggttttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
      601 cagcaacgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
      661 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
      721 cagaccaaca aggccaattg ccacaataag gaaaactacc aaaagagctt cgtacataat
      781 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
      841 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
      901 aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgccgaga
      961 aaaaaggcga caatcgccac ctttttagcc agttatctaa cgctttaaac ggcttttact
     1021 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
     1081 acgcgaatta acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc
     1141 gccgcttcaa cctctgcggt cacagctttg actgattcat gctccagcgg atcgccgcta
     1201 cctgcggtgt aacgcacgtt taccaggatt tgcgggaaca ttttcatgcc gctgcaaagg
     1261 tcgtgcaggc tcatatggtt acgcgccatc gccgccagca cctgcaaacc agcaacgatg
     1321 ccgtcaccgg tagtggtttt atccagcagg atcacatgac cggaattctc tgcaccgata
     1381 cgccagcctt tctcctgcat tttttccagt acgtagcggt cacccacttt cgcgcgcgca
     1441 aatggaattc ctaactgttt caacgccagt tcaagcccca tgttgctcat caatgtaccc
     1501 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
     1561 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccgtcgccg
     1621 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cgcgcgaacg
     1681 tcggtagccc ccacttcggc attgatgttt acaccgttcg gctcacaacc gatagcgata
     1741 acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
     1801 caatccacca caatcttcag ttcactaagg ctaagttcgt tcgggaacgt ggctttgcaa
     1861 aactcgatat agcgacccgc agcatcaacg atacggctgg ctttacccag ttctgccgaa
     1921 tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc
     1981 agtttggtgc cgtcgataga gaagaattta atgccattat cgtagaacgg gttatgcgat
     2041 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
     2101 gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt
     2161 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
     2221 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggggtg
     2281 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
     2341 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
     2401 ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca
     2461 ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga
     2521 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
     2581 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
     2641 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
     2701 cacttctgca aagacatcgt catacttcgg agcttcctgc atggttttgg gatttccctg
     2761 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
     2821 ggaaagggag cggatatcat taataatgtg agcgccaact tttgctgact cacggatgac
     2881 ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
     2941 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt
     3001 ggactcgcca ccaacgtcaa tgatcgtcgc gccagcgttg atcatcagat tcgcatgttt
     3061 caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt
     3121 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt
     3181 catgatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt
     3241 tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
     3301 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca
     3361 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
     3421 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
     3481 tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag
     3541 ttacgttcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
     3601 tgtttcgctt tcgctacgct acggccgagg aacacttcac cttcttcttc cgcgtacagc
     3661 agtggaccca atttctcaga gaagccccac tgagtcacca tgttacgtgc caggttggtc
     3721 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccgta gatgatctct
     3781 tctgccagac ggccgccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg
     3841 ctgattgcgt cgccctcagg caggaagaaa gtcacaccca acgcacgacc gcgtgggata
     3901 atcgtcactt tgtgcaccgg atcgtgctcc ggcaccaggc gaccgataat cgcatgaccc
     3961 gcttcgtggt aagccgtcga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
     4021 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
     4081 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
     4141 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggtgccaa tggtacgcga
     4201 cgcatgtgaa ccttcaggat ctgctcacga ccgcgaacat ctggcaagcc aaccacaacc
     4261 tgacggtcga aacggccagg acgcagcagc gctgggtcga gaacgtccgg acggttagtc
     4321 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
     4381 ttcagagtct gttcacgttc atcgtgacca ccgcccagac cagcgccacg ctggcggcct
     4441 acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac
     4501 atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag
     4561 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcag cgttttaccg
     4621 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
     4681 cggctcggct cgcgcagata ctcaaccagt tcagcaactt cttcttttgc ttcgtcgcag
     4741 cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc
     4801 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
     4861 cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
     4921 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtagt
     4981 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
     5041 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
     5101 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
     5161 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
     5221 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
     5281 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
     5341 tcccgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
     5401 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
     5461 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
     5521 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
     5581 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
     5641 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
     5701 atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca
     5761 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
     5821 agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac atatttatcg
     5881 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
     5941 ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt
     6001 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
     6061 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
     6121 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
     6181 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac
     6241 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
     6301 aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta
     6361 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
     6421 aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa
     6481 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
     6541 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
     6601 gccacgagca ataggtgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
     6661 gccaacgatg cgataagtct gttcttcgtc agaatcgaga ttcagcaccg ttacggtagc
     6721 accaaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgttcga
     6781 cagcttggct tcgatgtctt taatacggcc ttcgcagaaa ccctgctgtt cacgagctgc
     6841 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
     6901 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
     6961 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
     7021 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
     7081 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatagtc
     7141 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
     7201 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
     7261 attgaccagc tgtatagcgt tcagtgttca ggccgcaaat gttgatgagt acattactca
     7321 actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc
     7381 tattgattac cacagtcagc agatggcgct gcccgccagt acccagaaag tgattactgc
     7441 gctggcggcg ttgattcaac tcggccccga ttttcgtttt accacgacgc ttgaaaccaa
     7501 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
     7561 gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
     7621 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
     7681 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ccgccatagt
     7741 tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aaacctggtg atatggcttt
     7801 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
     7861 tggttctgcc gaagcgcaat actgcgaact ggatgtcgta ccaggcgacc tgaaccgctt
     7921 tacgctgacg ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttgccgtgca
     7981 ggatggagcc agctatgccg gtgcaattct gaaagatgag ttaaaacagg cgggtatcac
     8041 ctggagcgga acactgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag
     8101 taaacagtcg gccccgctgc acgatctgct taagattatg ctgaaaaagt cggacaacat
     8161 gatcgccgat acggttttcc gcatgatagg ccatacgcgc ttcaatgtgc ctggaacatg
     8221 gcgggccggg tcggacgctg tgcgtcagat cctgcgccag caagccggtg tcgatattgg
     8281 aaacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
     8341 catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat
     8401 gctgccgctg gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
     8461 cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
     8521 attcattacc acagcgagcg ggcaacgaat ggcgtttgta caatatcttt ctggctatgc
     8581 agtagaaccg gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt
     8641 gtataaagat atttatcaga acaattagtc aaaaagaaac cccggcacat ggccggggtt
     8701 ttgctgatta acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg tcccagtcgt
     8761 catcccagtc ctcatcatcc tcttcagcaa tctcttcaag ctgctggcga tgataatcat
     8821 cccacatgaa ttcgactttc tctggctgtt tcgcttcttc agcctgcacg accgggtttt
     8881 caatgataaa ggtcatcaca tcccagcaga gatctttcac gcccagtcca ctcgccgcag
     8941 agatcagata atatttatct tcccagccca gcgcttcagc gatcgctttc gctttctctt
     9001 cggcttctgc cttatccagc agatcgatct tattgaagac taaccaacgc ggtttcgccg
     9061 ccagatcctg gctgtatttt tccagctcgc tgataataat acgcgcgttt tcaaccggat
     9121 cggtgccgtc aatcggatcg atatcgatga ggtgcaacag gacgcggcaa cgttccaggt
     9181 gcttcaggaa gcgaatgccc agacctgcgc cttccgcagc gccttcaatc agtcctggaa
     9241 tatcggcaac aacgaagctc ttttcgttgt ccattcgtac cacacccaga cttggcacca
     9301 gagtggtaaa cggataatcc gccactttcg gtttagccgc cgataccgca cgaataaagg
     9361 tcgatttacc cgcgtttggc atccccaaca taccgacgtc agccagcagc atcagctcca
     9421 gcagcagctc acgcttatcg cccggcgtac cgttagtttt ctgccgtggt gtacggttaa
     9481 ctgacgattt gaaacgggta ttgcccagac cgtgccagcc gcccttagca accagcagac
     9541 gctgaccgtg tttggtcata tcgcccatgg tttcaccagt accctggtcg attacacgcg
     9601 tacctaccgg cactttaatt gtgacgtctt taccgcgctt accggtacag tcgcggcttg
     9661 cgccattctg accgcgctct gcacggaaag atttttcaaa acgataatcg ataagcgtgt
     9721 tcaggttctc gtcggcttcc atccatacgt caccaccatc accgccgtcg ccgccatccg
     9781 ggccgccttt cggaatatac ttttcgcggc ggaagctcac gcaaccatta ccgccatcac
     9841 ctgcaacgac cagaatcgat gcttcatcaa caaacttcat tttattctcc gtaaatcatt
     9901 cgcctgcgcg gggttgcgat accaccgttg tatgcttacg taatccgccc caaatacgat
     9961 gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga taacctaaaa
    10021 ggtttaacat cggtctggcg aagaaatcgg gccaggccag tgataaaaga tctgaaaaaa
    10081 acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac ctgcgctgcc tgccagcgag
    10141 ccatcgcttc cgccagggcg ccatatccta ccaaggtatt cagtccgcaa aaaattaaac
    10201 atgcgagctg ccagtggcta agctgcgcta tcactccagg ctttgccaga gggaagagcg
    10261 caattgtaca taaagtgtac agtaaaaaca ggatctgcgg tgaggccagc cgacgcaata
    10321 aaaccttttg cgccacgcca taactcaccc aaaccgtcgc cgcaccgacc ccaaagataa
    10381 ctccccaggt gtaatcggtt agcttggtaa atatctcgac cagactggtg ttaaaaaaca
    10441 tcaccaggcc gctcaggagc atcaatgccc ctacaacctg agtgctgcgc attttctctt
    10501 tcaggataaa tacgctggca accatcatgc caactggcga gagttgccca atcacctgcg
    10561 aagcggtcgg actcaggtat tgcaaggatg agctgaacag gatgaagttc ccaaacagcc
    10621 cggcggtcgc cactgccaac aaaatcaacc agcgtggctt acgaaacacg cgtaatggcg
    10681 gcaacctctt cttcaccgca agaatggcac ccaggccaat actcgccatc aagaaacggt
    10741 aaaacacgat tgtcggaggt tccatcacct ccagcacctg cttcattgcg attggcaacg
    10801 ccccccagca aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc tgctgcttca
    10861 taccgtattc cctgcaaagc gcatttgcgg attaacgtcg caccatgcgc atttttaccg
    10921 gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt accgggacgc
    10981 gaaaaactta ttcagcttcg atgctgataa atttacggtt tttcgggcct ttaacttcga
    11041 atttcacttt accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa cctacgttag
    11101 cgccagcgtg gaatttggta ccacgttgac gaacgatgat gctacccgcc agaacggatt
    11161 cgccaccgaa acgcttaacg cccaggcgtt tagcttctga atcgcgaccg ttacgtgtgg
    11221 agccgccagc ctttttatgt gccatttgaa atctctcctc aggtcttagg cgctgatgcc
    11281 agtaattttc acatcagtga accactgacg atggccctgc tgcttacgat agtgtttacg
    11341 acgacgaaac ttaacgattt taactttctc gccacgaccg tgagcaacaa cttcagcttt
    11401 gattacgccg ccatcaacga aaggaacgcc gattttgact tcttcaccgt ttgcgatcat
    11461 cagcacttca gcgaactcaa cagtttcgcc agttgcgatg tccagctttt ccaggcgaac
    11521 ggtctgacct tcgcttactc ggtgttgttt accaccactt tggaaaaccg cgtacatata
    11581 aaactccgct tccgcgcaca ccttttcaat gattcagagt gcgctataaa tattcacaat
    11641 agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt gctacagtca atacacgaag
    11701 aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca ttttttcagt acaatcaccc
    11761 tatattccta accataaacc ctaagttgcc tttgttcaca gtaaggtaat cggggcgaaa
    11821 agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca agatatggcg
    11881 ggtgttaatg cggcaatcct tgagcagctt aattccgacg tccaactgat caatcagtta
    11941 ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc cgatgattgc tgtactggct
    12001 gcacgagctg ttggctatga gggaaatgcg catgtcacca ttgctgccct gatcgagttt
    12061 atccacacgg cgactctgct acacgacgac gttgtggatg aatcagatat gcgcaggggt
    12121 aaagctaccg ccaacgccgc atttggcaat gccgccagcg tgctggtagg cgattttatt
    12181 tatacccgcg cgttccagat gatgaccagc ctcggttcgc tcaaagtgct ggaagtcatg
    12241 tcagaagccg taaacgtcat cgcagagggt gaagttctgc aactgatgaa cgttaacgat
    12301 ccggacatca ctgaagaaaa ctacatgcgc gttatctata gcaaaaccgc gcgtctgttt
    12361 gaggctgccg cgcagtgttc cggaattctg gcgggctgta cgccgaagga agagaaaggc
    12421 ctgcaggatt atgggcgcta tctcggcact gctttccagt tgatcgacga tttactcgat
    12481 tacaatgccg atggcgaaca gttaggtaaa aacgtcggcg acgatctgaa cgaaggtaaa
    12541 ccgacgctgc cgctgctgca tgcgatgcat catggcacac cagaacaggc acagatgatc
    12601 cgtaccgcca tcgaacaggg taacggtcgc catcttctgg aaccggttct ggaagcaatg
    12661 aacgcttgtg gatctcttga atggacgcgt cagcgcgccg aggaagaagc agacaaagcc
    12721 atcgcagcgt tacaggtgct cccggacacc ccttggcgag aagcactcat cggcctcgcg
    12781 cacatcgctg ttcaacgcga tcgttaatcc cctcccctca tcccgcgcag tgcgcgggat
    12841 gattccagta aactccataa gaatacttat tcagcgtaat aatgcctgaa aaaccagtgt
    12901 atacatcttt atatattctg aatattcaca ctctttacag gaacttttta gagcaatcgg
    12961 ccatcaggag tatagtgatg ctcgacagaa gaagtgttct gaatgaaagc gactaactta
    13021 aggagtgagg aaagatggaa agtaatttca ttgactggca tcccgctgac atcattgcgg
    13081 gtttgcgcaa gaagggaacg tcaatggcgg cggaatctcg cagaaatggt ttgagttcct
    13141 caacgctggc gaacgcatta tcgcgcccat ggccgaaagg agagatgatt attgcgaaag
    13201 ccctgggaac tgacccctgg gttatatggc catcacgcta ccatgatccg cagacccatg
    13261 agtttatcga cagaacgcag ttgatgcgta gctacactaa accgaaaaaa tgagtggtct
    13321 ggcggtagcc ccgcgaacgg ggctgccagc tctcagacga ttactcgcct ttcacacgct
    13381 caatatttgc acctaaagcg cgcagtttgt cttcaatgcg ttcgtagcca cgatcgatgt
    13441 gataaatacg atcaaccacc gtcgtccctt ccgcaataca gccagccagc accaggcttg
    13501 ctgatgcacg cagatcggtt gccataacct gtgcgccaga aagtttttca acaccgtgac
    13561 aaataacggt attgctttcg atttcggcgt gcgcgcccat acggctcagc tctggcacat
    13621 gcataaagcg gttttcaaaa accgtttcgg tgatgaatcc ggtcccttct gccaccaggt
    13681 tcaacagcgt gaactgggcc tgcatatcgg tcgggaatgc cggatgcggc gcagtacgta
    13741 cgttaacagc cttcggacgt ttgccatgca tatccaggct aatccagtct tcgccgactt
    13801 cgatgtccgc tccagcatca cgcagtttcg ccagcacggc gtcgagagta tctggctgcg
    13861 cgttacggca gataattttg ccgcgagaga tcgccgccgc caccaggaaa gtaccggttt
    13921 cgatacgatc cggcagcacg cgatagacac cgccgcctaa acgttccaca ccttcgatga
    13981 cgatacgatc ggtgccctga ccgctaattt tcgcacccag cgtaatcagg aagttcgcgg
    14041 tatcgacgat ttccggttca cgcgctgcat tttcaataat cgtggtgcct tccgccaggg
    14101 ttgcagcaca catgatggtc accgttgcgc caacgctgac tttatccatc acgatatgtg
    14161 cgcctttcag acgaccatcg acggaagctt taacgtaacc ttcttccagt ttgatggtcg
    14221 cgcctaattg ttcgaggcca gaaatgtgta gatcaaccgg acgcgcaccg atcgtacaac
    14281 cgccaggtag tgaaacttgc ccctgaccaa agcgcgctac cagcggcccc agtgcccaga
    14341 tagaagcacg catggtttta accagatcgt aaggtgcgca gaatacatta acgtcgcggg
    14401 catcaatatg cacagaacca ttacgttcta ctttcgcacc cagctggctt agcagcttca
    14461 ttgatgtatc gacgtctttc agtttcggga cgttctggat ctctaccggt tcttccgcca
    14521 gtagagcggc aaaaaggata ggcagagcag catttttagc gccggaaatt gtgacttcgc
    14581 cctggagctt cgttggcccc tgaacacgaa atttatccat ttagtttgtt ctcagttaac
    14641 aattcatatc cgctaccggc gaatcgccca tagctcaaaa gccgttcagt ttgcgatcgc
    14701 gcgcccactc cgcaggggta tacgctttga tcgacacagc atgaatgcgg ttatccgcaa
    14761 tatattccat cagcggacca tagaccgtct gctgtttttt aacccgactc atgccgtcaa
    14821 acaactcacc cacggcaata acctgaaagt ggctgccatc gccggaaacg tggacttcct
    14881 ggagggagag agcgttcatc aacacgctct gaatttcatt attttccatg ggatcttcaa
    14941 tcatcagtta ataaaccagc gaaacatctt agagcaaagt tgcgctggca taaataagca
    15001 aaaagcctcg ctgataaatc agacaaggct cgacttgcag gcaggtttgc cggacaggcg
    15061 gttaacgcca tatccggcct gaaaaaattt aacggggcag aacatcagca ggcaaattat
    15121 acaatttcgc cagggtatac actttgtcgt ttaccccctg aagcgtcaca ttgttgccct
    15181 gctttttcgc cagatcgata agatggagca gcagtgccag tccacccgta tccacgcggg
    15241 acacacggct aagatcgatg caggtaaccc ccttcaccgc ttcctcacgc atttcccaaa
    15301 gcggtagcag aacgtcctga tccagctctc cggataacgc cagcgtgtca cccgtctgca
    15361 tccagctcag tgactcgctc attatttttt ctcttccaga gtgattttct gttgagaaat
    15421 cgatttcagt tgcgcagtca ggccgtcgat acctttggta cgcagcagcg ttccccactc
    15481 gttttgtttg gtggtgatca tactgacgcc ttcagcaatc atgtcgtaag cctgccagtt
    15541 gcccgtctgg gagtttttac gccactggaa gtccagacgc accggcgggc ggccattcgg
    15601 gtcaataatg gtaacgcgaa taggcacaat ggttttatca cccagcggct gttctggcgc
    15661 aatctgatag gtttgaccgt gatacatcgc cagcgcctga ccgtaagcct gcttcaggta
    15721 ctcacggaaa gcggcaaagt aggcttcacg ttgagctggg gtcgcactct tgtaatactg
    15781 gcccagcacc agcgcaccgg cgtatttcac ctgtacgtat ggcagcagtt cctgatcaac
    15841 aatggtacgc agataatccg ggttggcccg aatttgcggt tgctcattct tcaggcgatc
    15901 gaacgttttc tgcgccgcct cgtccatcag cttatacgga ttggtctggt ctgccgcggt
    15961 tgccgcactc agcggtgcaa tcaccagcaa agcgaccatc attaaacgtt taaacatgcg
    16021 tcggttctcc tgaaattatt tcgttgtacc cacaggttca gtggtttcat tattacctgg
    16081 cgcagcagct ggcgcatcgc cactattctt attgtcatcg cctttactac cgtaaaggaa
    16141 ctgaccaatg agatcttcca gcaccatcgc agacttggtg tcctgaattg tatcgccatc
    16201 cttcaggata gcagtcccca gttccgggtc ttcaaaaccg acgtttaatg ccagatattg
    16261 ttcccccagc aggccggaag tacgaatgct cagcgaactg gtatcaggaa tatggttata
    16321 acgttgttca atttccagcg ttacgcgcgg cagataggtt ttcgggtcca gcgtaatatc
    16381 cgccacccgg cccacaacaa cgccaccaat actgaccgga gagcgcgctt tcaggccgcc
    16441 aatgttatcg aacgtcgcat aaagcgtata ggtcggttca gtacgtattg acgtcacgtt
    16501 cgccgccttc aggcaaacaa acagcgccgc cagcagtgct gctaataaaa agatacccac
    16561 ccaaatttca ttttttttcg tttgcatgaa ctcaattccc aaacatcaat gcggtcagca
    16621 caaaatccag ccccagaaca gccagagacg agtggacaac ggtgcgagtg gttgcccggc
    16681 taatcccggc agacgtcggg atggcgtcgt agccgttaaa caacgaaatc cacgtcaccg
    16741 tgatggcgaa caccacgctc ttaatcagac agttgaccag atccatacgc cagtcgacgg
    16801 cattttgcat tgccgaccag aagaacccgc tatcaatgcc tttccaactg acgccgacca
    16861 gcgatccgcc ccagatcccc acggcgacga agataaccgt caacagtggt aatgaaataa
    16921 ccccagccca gaaacgggga gaaataaccc gacgcagcgg atccaccgcc atcatctcca
    16981 tactggagag ttgctctgta gcgcgcatca ggccgatttc tgcggttaac gccgaaccag
    17041 cacgcccggc aaacaacagc gcggcaacca ccggccccag ttcacgcagt agcgataacg
    17101 ccaccagcat acccagactg gtttccgcac tataagtggt cagaaccaga taaccttgca
    17161 gccccaacac cattccgatg aacacgccag aaactacaat aatcagcatc gacaggacgc
    17221 cgacattata gagctggcgc accagcagcg gcgcatgttt gcgaaattcc ggtttgccga
    17281 ccagcgcatt gaataacatt aacccggccc gcccgaacgt tctcagggtt ttaatccctt
    17341 tatgtccgag cgacgccagc gcatttaaca gcatgagtgg cttaactccc tggtaaaaga
    17401 tcagcgtgat aatcgccggc aggatagcgg aacggaacag gcccgtcagc tatcccgtcc
    17461 agaaactgac gtacgcgcgg atcaggattc gcctgcaacg ccttggcact gccatgagcg
    17521 acaatttttt tgtccgccag gatccaggcg tgatccgcaa tacttaacac ttccggcaca
    17581 tcgtgagaaa ccaccacaca agtcacgccc agcgcgctgt tcagctcaga aatcagcttc
    17641 accagtacgc ccatggtaat gggatcttgc ccaacaaagg gctcatcaaa catgatgaga
    17701 tccggctcca gcgcaatcgc acgcgccagc gctgcacgcc gcgccatccc accggaaagt
    17761 tcagaaggca ttagtttagc cgctccacgc agccccacgg cctccagctt catcatcacc
    17821 gtactatgca acaatggcgc gggaagttgg gtatgttcgc gcagtggata ggcgacgttg
    17881 tcaaatacgt tcatatcagt gaacaacgcc ccggactgaa ataacatgct catccgtttg
    17941 cgcactgtat acaggcgcga acgagacatc gccggaatat tctcaccatc gaaaaggatc
    18001 tcaccatgat ctggtgcgat ttgcccgcca atcagacgga gtagcgtcgt tttaccgatg
    18061 cccgatggcc ccatgatcgc cgtgatcttc cctcgcggca cggtcaggga aatattatcg
    18121 aagatgcagc gattgccacg cgtaaaactg acatcgcgca tatcgactaa attcgccaca
    18181 gactgctcca taattcaccc ttcgtcttgc gttgattttc taagcatggc gctcaattta
    18241 accttgaacc caacatattt acagaatatt acccgccgtg gttagcgaaa gctggcattt
    18301 gttttacttt ttagccgcat aaagtcaaaa ttaagaatcc gttacggctt tctgaaaatc
    18361 ttcagcggac cggcgagtat acctgaagaa aggacgttag atgcttttag ctacggcact
    18421 gttaattgtt ggtttacttt tggtcgttta cagtgccgac cgcctggttt ttgccgcgtc
    18481 tattctttgc cgaacctttg gcatcccgcc gctgatcatc ggcatgacgg tggtcagtat
    18541 tggtacatcg ttaccagaaa tcatcgtctc gcttgccgcg tctctgcacg aacaacgcga
    18601 tttagccgtt ggtacagccc tcggctcaaa cattatcaat atattgctga tcctcggtct
    18661 ggctgcgctg gttcgtcctt ttaccgtcca ttctgatgtt ctacgccgtg aattaccctt
    18721 aatgttgttg gtcagcgtgg tggccggttc cgtactctat gacggacaac ttagtcgcag
    18781 cgatggtatc tttctcctct ttctggctgt gctatggctg ctgttcattg ttaaacttgc
    18841 acgtcaggct gaacgtcagg ggactgacag cctgaccaga gagcagcttg cagagctgcc
    18901 gcgtgacggc ggattgcccg tcgcgttttt atggctcggc attgcgctta tcatcatgcc
    18961 agtggccacg cggatggtgg ttgataacgc cacggtgctg gcgaattact ttgccatcag
    19021 cgagttgacg atgggtctga cggcaattgc tatcggaacc agcctgccgg aactggcaac
    19081 cgcaatcgcg ggggttcgca aaggtgaaaa cgacattgct gtcggaaata tcattggcgc
    19141 aaacattttt aatattgtca tcgtgttggg tttacccgcg ctgataacgc caggagagat
    19201 tgatccactg gcgtacagtc gtgactacag cgtgatgttg ctggtgagca ttatttttgc
    19261 gttgctgtgc tggcggcgct ccccgcaacc gggccgtggt gtaggggtat tattaactgg
    19321 cggatttatc gtatggctgg cgatgttgta ctggttatcg ccaatactcg ttgaataact
    19381 ggaaacgcat tatgtcgcac gtagagttac aaccgggttt tgactttcag caagcaggta
    19441 aagaagtcct ggcgattgaa cgtgaatgcc tggcggagct tgatcaatac atcaatcaga
    19501 atttcacgct tgcctgtgaa aagatgttct ggtgtaaagg gaaagttgtc gtcatgggga
    19561 tggggaaatc ggggcacatc gggcgcaaaa tggccgcaac gtttgccagc accggtacac
    19621 cttcattttt cgtccatcct ggtgaagccg cgcatggtga tttaggcatg gtcaccccac
    19681 aggatgtggt gattgctatc tctaactcag gtgaatccag cgaaatcact gccttaattc
    19741 cagtgcttaa gcgtcttcac gtaccgttaa tctgcatcac cggtcgcccg gagagcagca
    19801 tggcgcgcgc cgcagatgtg catctgtgtg ttaaagtagc gaaagaagcc tgtccgttag
    19861 ggctggcacc gaccagcagc accaccgcca cgctggttat gggcgatgcc ctcgctgtcg
    19921 cgctgttaaa agcacgcggc tttactgctg aagattttgc gctctcacac ccaggcggcg
    19981 cactgggtcg taaacttctg ctgcgcgtaa acgatattat gcatacgggc gatgagatcc
    20041 cgcatgttaa gaaaacggcc agtctgcgtg acgcattgct ggaagttacc cgcaaaaatc
    20101 ttggtatgac tgtcatttgc gatgacaata tgatgattga aggcatcttt accgacggtg
    20161 atttacgccg tgtcttcgat atgggcgtgg atgttcgtca gttaagtatt gccgatgtga
    20221 tgacgccggg gggaatacgc gtgcgccctg gcattctggc cgttgaggca ctgaacttaa
    20281 tgcagtcccg ccatatcacc tccgtgatgg ttgccgatgg cgaccattta ctcggtgtgt
    20341 tacatatgca tgatttactg cgtgcaggcg tagtgtaaag attcaaggat aaacaacaat
    20401 gagcaaagca ggtgcgtcgc ttgcgacctg ttacggccct gtcagcgccg acgttatagc
    20461 aaaagcagag aacattcgtc tgctaatcct cgacgttgat ggcgtactgt cagatggcct
    20521 gatttatatg ggcaataacg gcgaagagct gaaagcgttc aatgttcgtg acggttatgg
    20581 cattcgttgt gcgctcacct ctgatattga agtcgctatc attaccgggc gaaaggctaa
    20641 actggtagaa gatcgttgtg ccacattggg gatcactcac ttgtatcagg ggcagtcaaa
    20701 caaactgatc gcctttagcg atctgctgga aaaattggcg attgccccgg aaaatgtggc
    20761 ttatgtcggc gatgatctca tcgattggcc ggtaatggaa aaagtgggtt taagcgtcgc
    20821 cgtggccgat gcgcatccac tgttgatccc gcgcgccgat tacgtgacgc gcattgctgg
    20881 cggtcgtggc gcagtgcgag aagtttgcga cttattactc ctggcgcagg gcaaactgga
    20941 tgaagccaaa gggcaatcga tatgagtaaa gccagacgtt gggttatcat tgtgctatca
    21001 ctggcggttc tggtgatgat cggtattaat atggccgaaa aagacgatac cgcccaggtg
    21061 gtcgtcaaca acaatgatcc cacctataaa agcgagcata cggacacgct cgtctataac
    21121 ccagaagggg cgctaagcta tcgattgatt gctcaacacg ttgaatatta ttccgatcag
    21181 gccgtttcgt ggtttacgca gccggtactt accacgtttg ataaggataa aatcccgaca
    21241 tggtccgtaa aagcagataa agccaagctg accaatgacc ggatgctcta tttatatgga
    21301 cacgttgaag tcaacgcact tgtgccggac tctcaacttc gcagaatcac gacggataac
    21361 gcgcagatca atctggtgac gcaggatgtt acctctgaag acctcgtcac gttatacgga
    21421 acaacattta actccagcgg tctgaaaatg cgcggcaact tacgcagcaa gaacgccgag
    21481 ctgattgaaa aggttagaac atcctatgaa attcaaaaca aacaaactca gccttaatct
    21541 tgtgcttgcc agctcacttc tggccgccag cattccggca tttgccgtaa ccggagacac
    21601 tgatcagccg atccacattg aatcggacca gcaatctctt gatatgcaag gcaacgtggt
    21661 tacctttacc ggtaatgtca tcgtcaccca gggcaccatc aaaattaatg ccgacaaagt
    21721 ggtcgttacc cgtccgggcg gcgaacaagg taaagaagtg attgacggct acggtaaacc
    21781 ggcaacgttc taccagatgc aggacaacgg taaacccgtt gaaggtcacg cttcccagat
    21841 gcactacgaa ctggcaaaag attttgtcgt tctgacgggt aatgcttatc tgcagcaggt
    21901 cgatagcaac attaagggcg ataagatcac ttacctggtg aaagagcaga aaatgcaggc
    21961 tttcagcgac aaaggcaagc gcgtaaccac cgttctggta ccgtcgcagc tgcaggacaa
    22021 aaacaacaaa ggccagaccc cggcacagaa gaagggtaat taattcgtta tggcaacatt
    22081 aactgcaaag aaccttgcaa aagcctataa aggccgtcgc gtggtagaag acgtcagcct
    22141 gaccgtcaac tccggggaaa ttgtcggtct gctggggcca aacggtgccg gtaagaccac
    22201 cactttctac atggttgtag gcattgtgcc gcgcgatgcg ggcaacatca ttattgatga
    22261 tgacgatatc agtctgctgc ctctgcatgc acgcgcgcgc cgcggtatcg gctatctgcc
    22321 acaggaagcc tccattttcc gtcgcctcag cgtttacgat aacctgatgg cggtactgca
    22381 aattcgtgac gacttgtctg ctgaacaacg tgaagaccgc gcgaacgagc tgatggaaga
    22441 gtttcacatt gagcacctgc gtgacagcat ggggcagtca ctctccgggg gtgaacgtcg
    22501 ccgtgtagaa attgcccgcg cactggctgc gaatccgaaa tttattctgc tcgacgaacc
    22561 gtttgccggg gttgacccga tctcggttat cgacattaaa cgcatcattg agcacctgcg
    22621 cgacagcggc ctgggcgtgc tgatcactga ccacaacgtg cgtgaaacac tggcggtttg
    22681 tgaacgcgct tatatcgtca gtcaggggca tttgatcgcc cacggcacgc ctacagaaat
    22741 cttacaagac gaacacgtta agcgtgtata ccttggggaa gacttcagac tctgataggg
    22801 tagaagtttg cgacgtttta gcaggagagt acgattctga acatgaagca aggtttgcaa
    22861 ctcaggctta gccaacaact ggcgatgacg ccacagcttc aacaggcaat tcgtctgttg
    22921 cagttgtcga cgctggaact tcagcaggag ctacagcagg cgctggagag taatccgctg
    22981 cttgagcaaa tcgacactca tgaagaaatc gacacccgcg aaacgcaaga cagtgaaacg
    23041 ctggacaccg ccgacgcgct cgaacaaaaa gagatgccgg aagagctgcc gctcgatgcc
    23101 agttgggata ccatttacac cgctggtaca ccatccggca ccagcggtga ctacattgac
    23161 gacgagctgc cggtctatca gggcgaaacg acgcagacct tgcaggatta cctgatgtgg
    23221 caggtcgagc tgacaccgtt ttccgacact gaccgcgcta ttgctacctc tatcgtcgat
    23281 gccgttgatg acaccggtta tctgactgtc ccgctggaag atattctcga aagtatgggc
    23341 gatgaagaga tcgacatcga cgaagttgaa gccgtcctta agcggatcca acggtttgat
    23401 ccggtcggtg tggcggcaaa agatctgcgt gactgcctgc tgatccaact ctcccaattc
    23461 gataaaacca cgccgtggct ggaagaggcc agactgatca ttagcgatca tctcgatctg
    23521 ttagccaatc acgacttccg cactttaatg cgcgtcacgc gtctgaaaga agatgtgctg
    23581 aaagaagccg tcaatctgat ccagtcgctc gatccgcgcc ccgggcaatc gatccagact
    23641 ggcgaacctg agtatgtcat tccagatgtg ctggtgcgta agcataacgg tcactggacg
    23701 gtagaactca acagtgacag cattccgcgt ctgcaaatca accagcacta cgcctcgatg
    23761 tgcaataacg cgcgcaacga tggtgatagc cagtttatcc gcagcaatct gcaggatgcc
    23821 aaatggttga tcaagagtct ggaaagccgt aacgatacgc tactgcgcgt gagtcgctgt
    23881 atcgttgaac agcagcaagc cttctttgag caaggtgaag aatatatgaa accgatggta
    23941 ctggccgata tcgcccaggc tgtcgaaatg catgaatcga cgatatctcg cgtgaccacg
    24001 caaaaatacc tgcatagtcc acgaggcatt tttgaactga agtatttctt ttccagtcac
    24061 gtcaataccg agggcggcgg cgaagcttcc tccacggcga ttcgtgcgct ggtgaagaaa
    24121 ttaatcgcgg cggaaaaccc agcgaaaccg ttgagcgaca gcaagttaac ctctttgctg
    24181 tcggaacaag gtatcatggt ggcacgccgc actgttgcga agtaccgaga gtctttatcc
    24241 attccgccgt caaaccagcg taaacagctc gtttgaccca accgataagg aagacactat
    24301 gcagctcaac attaccggaa ataacgtcga gatcaccgag gcactgcgcg aatttgttac
    24361 agccaaattt gccaaacttg agcaatattt tgaccgaatc aaccaggtct atgttgttct
    24421 gaaagtggag aaagtcaccc acacctcaga tgcaacactg catgtaaacg gtggtgaaat
    24481 tcatgccagc gcggaaggtc aggacatgta cgctgccatt gatggcttaa ttgataagct
    24541 ggcacgccag ttgaccaaac ataaagataa actgaaacaa cactaattgt ccgggcaatt
    24601 agcatgtgca tggcggcctg ttgtgcggca caacgggcca tttgtacggt taatgctccg
    24661 agcctgttcc actgtttgag tgggcaggtt cttaggtgaa attatgacaa ataatgatac
    24721 aactctacag cttagcagtg ttcttaacag ggaatgtacg cgaagccgcg tccactgtca
    24781 gagcaaaaaa cgcgccctgg aaatcatcag cgagctggcg gcgaaacaac ttagcctgcc
    24841 gcctcaggtg gtttttgaag ctatcctgac gcgggaaaaa atgggcagta ccggtatcgg
    24901 caatggtatt gccattccgc atggcaaact ggaagaagat actctgcgtg ccgttggcgt
    24961 tttcgttcag ctcgaaacgc ctatagcttt cgatgccatc gacaaccaac cggtggactt
    25021 actttttgcc ctgctggtgc cggcagacca aactaaaacg cacctgcata ctctgtcgct
    25081 ggtggcgaaa cgtctggcgg acaaaaccat ctgccgccgt ttacgcgcag cccagagcga
    25141 tgaagagctg tatcaaatca ttacggatac cgaaggtact ccggatgaag cgtagttatt
    25201 cggtaatgtc tcttttagac gttgtgagga gaaacagtac atggtactga tgatcgtcag
    25261 cggacgttca ggttcaggta aatctgtcgc cctgcgtgcg ctggaagata tgggttttta
    25321 ctgcgtggat aaccttcccg tagtgttgtt acccgatctg gctcgaaccc tggccgatcg
    25381 cgagatttct gccgccgtca gcattgatgt tcgtaatatg ccggagtcac cagaaatatt
    25441 cgaacaggcg atgagtaacc tgcctgacgc tttctcaccg caactcctgt tcctggatgc
    25501 cgaccgtaat accttaattc gtcgttacag tgacacgcgc cgactgcatc cgctttccag
    25561 caaaaacctg tcgctggaaa gtgctatcga caaagaaagc gatctgctgg agcctctgcg
    25621 ttcgcgagcg gatctgattg tcgatacctc agaaatgtcc gttcacgagc tggcagaaat
    25681 gctgcgtacc cgtctgctgg gtaaacgtga acgcgaactg acaatggtct ttgagtcttt
    25741 cggcttcaaa cacggtatcc ctatcgatgc agattacgtc tttgacgtgc gcttcttgcc
    25801 gaacccgcac tgggatccga aactgcgtcc aatgacaggt cttgataagc ctgtcgccgc
    25861 gttcctcgac cgccacacag aagtacacaa ttttatctac cagacgcgaa gctatcttga
    25921 gctatggtta cctatgctgg aaaccaacaa ccgtagctac ctgacggtcg ccattggttg
    25981 taccggcggg aagcaccgtt cggtgtatat tgcagagcaa ctggctgact acttccgctc
    26041 gcgcggtaaa aacgtccagt cacgccatcg tacgctggaa aaacgtaaac catgaccgtc
    26101 aagcaaactg ttgaaatcac aaacaagctg ggcatgcatg cccggcctgc aatgaagctg
    26161 tttgaattaa tgcagggttt tgacgctgaa gtgctgttac gtaatgacga aggcaccgag
    26221 gctgaagcca acagcgttat tgcgctgctg atgttggatt ctgccaaagg acggcagatt
    26281 gaagttgaag cgaccggtcc acaggaagag gaagcactgg ccgccgttat cgccctcttt
    26341 aattctggtt ttgatgaaga ttaatcttca tcactttgac atacaactcc cttcaaactc
    26401 cccccgacaa taagaaaatc acgtactgaa atcgttctca atcaacgtca attgtacatt
    26461 tcgtgcgctt tttactttca gaagaacctt aagaaaacct taagaagcat tgtttaggtt
    26521 tcgtttaagt taatcgacca tactggagat cgtcagaaaa tatttccagg agatggcatg
    26581 attcgcttat ctgaacaaag tcccctgggc accggaagac atcggaaatg ttatgcgcat
    26641 ccggaagatg cccaacgctg tatcaagatt gtctaccatc gtggcgatgg tggcgataaa
    26701 gagatccgcc gtgagttaaa gtactacgcg catcttggtc gccggttaaa agactggagt
    26761 ggaataccgc gctatcacgg taccgttgag acggattgcg gaaccggtta tgtctacgat
    26821 gtgattgccg attttgacgg caagccctcc attacgctga ccgaatttgc tgaacaatgc
    26881 cgttacgaag aagacgttgc acaactgcgc caattactga aacagctgaa gcgttatttg
    26941 caggataacc gtatcgtgac gatgtcgctg aagccgcaga atatcctctg tcaccgcatt
    27001 agtgagtcag aggttatccc cgtcgtctgc gacaacattg gtgaaagaac attgattcca
    27061 ctggcgacct ggtcaaagtg gtgttgtctg cgtaagcaag agcggttgtg gaaacgattt
    27121 atcgcccaac cggcgctggc tattgccctg cagaaagatc tgcaacctcg tgagagcaaa
    27181 acgctggccc tcacttcgcg cgaagcttaa tccagctggt gttgttgcat aaacggctca
    27241 ccgcctaact gatacatctg ccgtaaaatc cacgcctgac ggctgcgcac gtagcccgat
    27301 ggtgcggaga ctttgaaacg aagtggatta ggtaatacag ctgccagtaa ggcagcttcc
    27361 gatcgggtaa gtttgctcgc gggtttgtgg aaatagcgtt gtgccgcagc ttcgacgcca
    27421 aacacaccgt cgccaaattc ggcgatattc aggtaaaccg tcaggatacg ctttttgctc
    27481 cagactgttt ctatccccag cgttaatccg gcttccagcc cttttcgaac ccagctacgc
    27541 ccatcccata aaaagagatt tttggccgtc tgctgagaaa tcgttgaagc accgcgaata
    27601 cggttttcat tgcgctcgtt gtgcgccagg gctttctcaa tggaagcgac atcaaagccc
    27661 cagcgctcag gaaatttctg atcttctgcg gcaatcaccg ccagtcccat ccacggcgag
    27721 atttgatcca tactgaccca gtcagaatgt gccacgtaac gaaaattgcc atgcagccag
    27781 gcgctgacct gtcgctcgac cattaccgct gagaaaggaa caggcgcaac gctaaacaac
    27841 gcgatgcccc cgccccagaa aacggcgagg acaaccatta accgcagaag aaaacggcga
    27901 acgaaactaa acaccgttaa gcggctttta ctcattcagc cagaaccagc acgcgggaaa
    27961 ccagcttatc aatgccgctc gccgcttctg caatgttctg cgccagcata tatgccgggg
    28021 tggtgacaat cttattgtct tcatcaacca cgatatcatc gacaggacac ggcacatgct
    28081 ctgcgcccat ctcttccagc acttctgcgg tatcgatatc agtaccgatg gtcaaacgca
    28141 gcgggaaatc gaaaattttc ggcagcatcg ccggggcaat acacataaaa ccaagcggtt
    28201 ttccggcctg atgcatcgct tgtgccagcg cctttaattc acggtcaacg gtgcattcgc
    28261 gaccaagact ggcaaaattg cttaaattct ttgccgcgcc aaaccccccc ggcacaatca
    28321 acgcatccag ttcagcggca tcggcctggg ccagaggacg gatttcaccg cgcgttattc
    28381 gtgccgcttc aatcagcaca ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga
    28441 taacatcaac ctgctgctta tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa
    28501 tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc gtcatagaca ccgcatccgc
    28561 tcagaattac gccaattttc ttcattgtga tcatcctttc gcaattgact gaaacacatt
    28621 aattttttta ataaaaatgg tacgcatcac acatttaact gattcatgta acaaatcatt
    28681 taagttttgc tatcttaact gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca
    28741 tcataataat ttacggcgca gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc
    28801 cggtctagcc ggggtcattt tttagtggct tttgccaccc acgctttcag cacttctacg
    28861 tcgtgacgcc actcttcttt catctcttca atccattcac caacgttatc ttcccaggca
    28921 ggaaggtcag gagactgaat ttgctgaccc agctgttgca gatggcgtaa ccccactgac
    28981 cccgccgcac ctttaatttt atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc
    29041 agattcgact ccagcacgct gacatagccc ggcatcatct tctcaaacac cgctaacccg
    29101 tcggtgatca gcttcggtcc tacaagttcg agatactgtt ccagcatggg aatatcgagc
    29161 aatgcttctg atttactgtt ctcttctgtc gtcaccgtac tctcctcatc atcctgggta
    29221 tcccagaatt tcttgatcat cgcggttagc gccggaacag aaagcggctt actcagcaca
    29281 tcatccattc cagcattgag gtactcttgc ttgtctttca gcacgttagc ggttaaagcc
    29341 accagcggcg gtaaatcctc gcgcggataa cgcttcgtca gttcacgaga gatatccagc
    29401 ccggtcatat ctggcaactg aatatccagc aacaccaggt cgtattcgcc cggtttaaac
    29461 atctccagcg ccgccttgcc ggtcatggcg acatcaacgc tgttacctaa tttttccagc
    29521 acagaacgcg cgacaatcac gttcagttca atgtcttcca ccagtagcac attcagcgcc
    29581 ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct cttctgccac cgacggtgcg
    29641 tggatcgtca acgtaaaggt tgaacctttg ccctgttcgc tggtaaccgt aatatcgccg
    29701 cccatatttt tcgccagacg acgagaaacg gccagaccaa taccggtgcc ggtggcaggt
    29761 ttaccgccat gactgtcttt cacctggtaa tacatggcga agattttatc cagctcatcc
    29821 tgcggaatgc cgataccaga atcttccact tcaaaatgca gcatatcgcc ttcatcgtag
    29881 cgcacgcgca cggtaacctg gccttgctgg gtgaatttga cggcgttact gatgaggttc
    29941 cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta
    30001 ggctccaggt taaagcgcaa tcctttttgt tgcgcctgca aggcggagag attttccaga
    30061 tcggcaagga agctggtgaa atcaaccggc tgattatcaa gctggacctt gcgccgttcc
    30121 atcttatcca tgtcgataat atcgttgaag atattcccca gcgtgacggc cgaaacatgg
    30181 atagttttga gatatttttc ctgctcggcg gtgagttcgg tatccagtag gatgcggctc
    30241 aggccgacga taccgttcag cggtgtacgc aattcgtgac tgatggtgga aataaacgtc
    30301 gttttgtcgc ggctggcgcg ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg
    30361 cgaccaaagc ccatcaaacc gtgacgttta cccacgcggt cgtagtacgg cactttacga
    30421 atttcaaagc aggctttgcg tccgtcggga taatccagcc actgctcata ggtcagtgac
    30481 acattatgac ggaacacttt ttcatcggtt tcaatgactt tcgcagcggc ttccggtgag
    30541 taaacatcag caggtttcag atgaaccagt tgtttttcgc tttttccggt cagcagctcc
    30601 atcgcgcggt tacagccgga aaactcttta tcttcgttac gataaaaaac caggtcgggt
    30661 gaagcatcaa ggaaggaacg taagaatgag gattgctgct cgagctgaat ttgtgtctct
    30721 tcgcgctctt tgatttcaat tttcagttgg ccgaaggttt cctgcagttc tgcttccgct
    30781 ttttcacgaa cggcaatttc ctgatttagc tgggcaatat tatcttttaa ctgaacgttg
    30841 aggctcaaat cgcgctcgcg catctcctcc agtttttgca ccagccgtga cagacgttgt
    30901 cgtgactcct ccagttgctc gacgaccacc gatagaaagt agaccgccca cggcgtaatc
    30961 agcaaaccaa agaagataga acgaataaca tcaatgcttt cgacctgacc atgcagcacc
    31021 atggttaccg ccatttgtac cacaatggca agaacgacga gggccagcgc cagcaacatt
    31081 gagaagcgca ccagacctaa cttcatcatc aggtcaacat aatactgcgc cagcagacga
    31141 atttgcttca ttagggaatt ccttcacgac aacctgcccc aataataccc aattctgaca
    31201 gttacgttat aaattgtgtg agaaatgcgg aattcccctc aatgacctac tccgtaggag
    31261 gtagccacgg acgtcccagc gccgatccct gtacgccatg ttcattcaga tagcgatcca
    31321 gctcgaccat tcccgtccat cgattttcgc accacagcgg agcaagcagc gtcgggcgac
    31381 gggcgctggc ggaaatgcgg tggtagatca cttccggtgg cgtatggcga atcatttctc
    31441 cggcagtgag tgtgtaatcc tccagttcaa taccgttcaa acgccccgct tcccaggctt
    31501 tcgccataat gctgcctttc acaatatgca gcggatgcag ctttatgccg tccacgccgg
    31561 tttcaaccac gcgctccagc gtttgcaaac attcagcctg cccttcgcca ggcaggccga
    31621 caattaagtg ggaacacacc ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat
    31681 aacaggcaaa atcatggccg cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta
    31741 gccccagctc cagccacact tcgtaaccct ggtccttata ttcgcaaagc agatccagca
    31801 ccgcatccgg cacgcagtcc gggcgggtac caacacacaa accgacaata ttggcctggc
    31861 tcaccgcctg ctgatacata gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg
    31921 cctgaaagta ggccagatag cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt
    31981 gctcggcaat ggaacgatgt tgctgcgctt catcagcaaa cgaggcaaca ttacagaatg
    32041 tgcagccgcc acgcccgatg gtaccgtcac ggttagggca gctaaaaccg ccatgcagcg
    32101 tcagcttatg caccttttgc ccataacgac gggtgagatc accaccaaac atattgacta
    32161 atttctgtaa ctgcataatc tgatagaccg cgccttgaaa agaggccaaa gcctgccatt
    32221 tttagctcat ttcggcgatg acctggatca atcgtcccgg cctgccttta ccaactgcat
    32281 aatcaatcaa aattaccgaa atttcatgca taatcacata aatcactttt gcttatcttg
    32341 tgtcagattt ttttatctcc ttatggattt taggcaaaaa cagtagcatg aaacgtcatt
    32401 accaattaag gcagtataaa atgctggttt tctcgtcagt tcaaggcagg ataagggtta
    32461 acacgccttt atgacagtca ggaattggct gtttc
//
LOCUS       DAZKFU020000023        32342 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZKFU020000023 DAZKFU020000000
VERSION     DAZKFU020000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.023"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(18..93)
                     /locus_tag="ACHOQQ_RS04360"
                     /old_locus_tag="pgaptmp_000872"
     tRNA            complement(18..93)
                     /locus_tag="ACHOQQ_RS04360"
                     /old_locus_tag="pgaptmp_000872"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="ACHOQQ_RS04365"
                     /old_locus_tag="pgaptmp_000873"
     tRNA            complement(152..228)
                     /locus_tag="ACHOQQ_RS04365"
                     /old_locus_tag="pgaptmp_000873"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHOQQ_RS04370"
                     /old_locus_tag="pgaptmp_000874"
     CDS             complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHOQQ_RS04370"
                     /old_locus_tag="pgaptmp_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4469166.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHOQQ_RS04375"
                     /old_locus_tag="pgaptmp_000875"
     CDS             complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHOQQ_RS04375"
                     /old_locus_tag="pgaptmp_000875"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGU4469167.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHOQQ_RS04380"
                     /old_locus_tag="pgaptmp_000876"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHOQQ_RS04380"
                     /old_locus_tag="pgaptmp_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGU4469168.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILIGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHOQQ_RS04385"
                     /old_locus_tag="pgaptmp_000877"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHOQQ_RS04385"
                     /old_locus_tag="pgaptmp_000877"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGU4469169.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDNCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKSR
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHOQQ_RS04390"
                     /old_locus_tag="pgaptmp_000878"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHOQQ_RS04390"
                     /old_locus_tag="pgaptmp_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGU4469170.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVIGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHOQQ_RS04395"
                     /old_locus_tag="pgaptmp_000879"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHOQQ_RS04395"
                     /old_locus_tag="pgaptmp_000879"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGU4469171.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHOQQ_RS04400"
                     /old_locus_tag="pgaptmp_000880"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHOQQ_RS04400"
                     /old_locus_tag="pgaptmp_000880"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGU4469172.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHOQQ_RS04405"
                     /old_locus_tag="pgaptmp_000881"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHOQQ_RS04405"
                     /old_locus_tag="pgaptmp_000881"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4469173.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHOQQ_RS04410"
                     /old_locus_tag="pgaptmp_000882"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHOQQ_RS04410"
                     /old_locus_tag="pgaptmp_000882"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4469174.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHOQQ_RS04415"
                     /old_locus_tag="pgaptmp_000883"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHOQQ_RS04415"
                     /old_locus_tag="pgaptmp_000883"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGU4469175.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHOQQ_RS04420"
                     /old_locus_tag="pgaptmp_000884"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHOQQ_RS04420"
                     /old_locus_tag="pgaptmp_000884"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU4469176.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHOQQ_RS04425"
                     /old_locus_tag="pgaptmp_000885"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHOQQ_RS04425"
                     /old_locus_tag="pgaptmp_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGU4469177.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHOQQ_RS04430"
                     /old_locus_tag="pgaptmp_000886"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHOQQ_RS04430"
                     /old_locus_tag="pgaptmp_000886"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGU4469178.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHOQQ_RS04435"
                     /old_locus_tag="pgaptmp_000887"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHOQQ_RS04435"
                     /old_locus_tag="pgaptmp_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGU4469179.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHOQQ_RS04440"
                     /old_locus_tag="pgaptmp_000888"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHOQQ_RS04440"
                     /old_locus_tag="pgaptmp_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGU4469180.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHOQQ_RS04445"
                     /old_locus_tag="pgaptmp_000889"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHOQQ_RS04445"
                     /old_locus_tag="pgaptmp_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGU4469181.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHOQQ_RS04450"
                     /old_locus_tag="pgaptmp_000890"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHOQQ_RS04450"
                     /old_locus_tag="pgaptmp_000890"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGU4469182.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="ACHOQQ_RS04455"
                     /old_locus_tag="pgaptmp_000891"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="ACHOQQ_RS04455"
                     /old_locus_tag="pgaptmp_000891"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGU4469183.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHOQQ_RS04460"
                     /old_locus_tag="pgaptmp_000892"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHOQQ_RS04460"
                     /old_locus_tag="pgaptmp_000892"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGU4469184.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22141..22590
                     /locus_tag="ACHOQQ_RS04465"
                     /old_locus_tag="pgaptmp_000893"
     CDS             22141..22590
                     /locus_tag="ACHOQQ_RS04465"
                     /old_locus_tag="pgaptmp_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU4469185.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFITTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNIINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22785..23066
                     /gene="ppiC"
                     /locus_tag="ACHOQQ_RS04470"
                     /old_locus_tag="pgaptmp_000894"
     CDS             22785..23066
                     /gene="ppiC"
                     /locus_tag="ACHOQQ_RS04470"
                     /old_locus_tag="pgaptmp_000894"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGU4469186.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23112..24587)
                     /gene="ilvC"
                     /locus_tag="ACHOQQ_RS04475"
                     /old_locus_tag="pgaptmp_000895"
     CDS             complement(23112..24587)
                     /gene="ilvC"
                     /locus_tag="ACHOQQ_RS04475"
                     /old_locus_tag="pgaptmp_000895"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGU4469187.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24737..25630
                     /gene="ilvY"
                     /locus_tag="ACHOQQ_RS04480"
                     /old_locus_tag="pgaptmp_000896"
     CDS             24737..25630
                     /gene="ilvY"
                     /locus_tag="ACHOQQ_RS04480"
                     /old_locus_tag="pgaptmp_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGU4469188.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(25682..27226)
                     /gene="ilvA"
                     /locus_tag="ACHOQQ_RS04485"
                     /old_locus_tag="pgaptmp_000897"
     CDS             complement(25682..27226)
                     /gene="ilvA"
                     /locus_tag="ACHOQQ_RS04485"
                     /old_locus_tag="pgaptmp_000897"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGU4469189.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27229..29079)
                     /gene="ilvD"
                     /locus_tag="ACHOQQ_RS04490"
                     /old_locus_tag="pgaptmp_000898"
     CDS             complement(27229..29079)
                     /gene="ilvD"
                     /locus_tag="ACHOQQ_RS04490"
                     /old_locus_tag="pgaptmp_000898"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGU4469190.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29144..30073)
                     /gene="ilvE"
                     /locus_tag="ACHOQQ_RS04495"
                     /old_locus_tag="pgaptmp_000899"
     CDS             complement(29144..30073)
                     /gene="ilvE"
                     /locus_tag="ACHOQQ_RS04495"
                     /old_locus_tag="pgaptmp_000899"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGU4469191.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30093..30356)
                     /gene="ilvM"
                     /locus_tag="ACHOQQ_RS04500"
                     /old_locus_tag="pgaptmp_000900"
     CDS             complement(30093..30356)
                     /gene="ilvM"
                     /locus_tag="ACHOQQ_RS04500"
                     /old_locus_tag="pgaptmp_000900"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGU4469192.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30353..31999)
                     /gene="ilvG"
                     /locus_tag="ACHOQQ_RS04505"
                     /old_locus_tag="pgaptmp_000901"
     CDS             complement(30353..31999)
                     /gene="ilvG"
                     /locus_tag="ACHOQQ_RS04505"
                     /old_locus_tag="pgaptmp_000901"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGU4469193.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLSTTKMPATCTLKGLGAVAADYSYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLSDEHAWRYDHPGDTIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32002..32052)
                     /gene="ilvX"
                     /locus_tag="ACHOQQ_RS04510"
                     /old_locus_tag="pgaptmp_000902"
     CDS             complement(32002..32052)
                     /gene="ilvX"
                     /locus_tag="ACHOQQ_RS04510"
                     /old_locus_tag="pgaptmp_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGU4469194.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32139..32237)
                     /gene="ilvL"
                     /locus_tag="ACHOQQ_RS04515"
                     /old_locus_tag="pgaptmp_000903"
     CDS             complement(32139..32237)
                     /gene="ilvL"
                     /locus_tag="ACHOQQ_RS04515"
                     /old_locus_tag="pgaptmp_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGU4469195.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         7814 a         8934 c         8182 g         7412 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgcg
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttcccgtg
      361 gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccaac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 gggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgcctatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcacc ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact ggacataaac
     1381 gccaatggcg gtgatttcag agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaagaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtaatgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagcccca gcgtttgcca gattttcggt gcgcgtttca
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaatactgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccaa agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccaacgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcgtgctgca
     2581 tatcacgcca accgatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgcg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagc gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt tggctgagtt
     3061 cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggcgagaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgatgcct atcaggatca cccacgtaag
     3661 attggtttcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacgtagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagaca acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgcaga tagttcgggc taaagaaggc aatggtgttt
     4081 ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcaccggcaa atgctgttcc gtcatatcct gccagaacgg attttcccgg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaca gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc gagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcagaac ttgcagattt tcttcgctga ataactccag cccagcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggcg cgcaaggcag caatatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt ttcccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagagtccat ccgcgtcggg aagtacagca gttcgccccg cacctttgga
     4621 tgcgttttgg caaaaaagct caaatcaccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagcgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 agatctgccc cccagatatg ccagaaaaac tggcgggact taatgccacc actcagcaaa
     4801 gccagccaca gtttgggatt gaactgaccg tggaagaaaa aacgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agttatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catataggcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg aagctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg atggcggcag
     5761 gaatcaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccaacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaagc
     6061 caattaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgggga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg tgctaacgca
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttcca
     6961 agcgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttctac cacaaacaga
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc ctattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg taaaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgactg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacta cagcaccgct atcactggca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcacccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacgcgt ctggcgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttccaccg
     8281 tctgtacaaa ggtgctggct tcaatcaggc tgtcgtgagt gccagtgtcc agccaagcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttccagcga gatagcgcgg aaattgtcgt caaactccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccaactttca ttagccagat accactgcac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 agcgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga taatgcacca
     9241 cgccttgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcgatttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taagggccgt agttattcga gcagttggtg ataagcgtcg
     9541 gcagaccata ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtca gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgttagcgc attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg cgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc gtcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atctttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agagtttcgc cgctatgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagccgc caggcaatca gccaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt cacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg tgggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 agtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagtt tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaag
    11821 ctttcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat aaacgggtaa
    11881 tttgccgcaa gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatctgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaaatgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg taaaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcga ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggcccgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgctt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttggtcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaacg tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgccgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagatcacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacacag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagc
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcattgccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtagataact tcgtccattt tagaaccagt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacgg
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttcgcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tggttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttccgtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcagccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccatgccga
    17281 tgcggaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg gtggtgatgg
    17341 ttacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caacaccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct attagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 tacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcctgaa
    18301 tcacttactg tagtaagtga ttcaggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa
    18481 aacgcaaagt gcgtctggct gctggcctga atagtgaaaa tgccctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat attcctccct
    18601 cgcaaattcg cgttgtcgct acggcgacgt tacgccttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatt ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cacgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggagccagt actgaactgg taaccggcac gggtgcacaa actacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacgctg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagcg ccgcaacacg
    19501 ctgcttatct ggtgcgtaat ctggatcttc ccggttttac ccccgcacag aaaaaattgc
    19561 tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc gccgcgcgtc gcagaacaac tctgccgttt actacgcctg gccatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aagccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ttccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 gctgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatgctgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc ctttcgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg tcaccacctg
    20341 ggttaacgtc atcggctcat ccagttcact gccttcgagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcacgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccattcac ccaacttttt tagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatggt taccgcgata
    20881 aagaatggcg taatctttgt actgcgtttt attgacgaag tgatgggcga tcagctcgcc
    20941 agtaacgcgc tcggcttcat gttcctcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc ggattattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctgac tcagcagcac caggttttgt ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcgtcat cgcccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagc tcatactggc tggtgttggt gtcctgatac tcatccacca gcagatagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagcagca acgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagaacgtt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac gatcccgctc gccctttgcc tctgccgccg cctgcgccgg
    21481 tgttttgaga tcattcttcc agttagagat ggtcgaaatc agttgttgca ggagaacttt
    21541 gtcatcttca atcagcccct cggtcaactc tttaagcaaa gcaagctgat cggtatcgtc
    21601 aaacaacgag aagttcgctt tcatcccaag cgccgcatac tcgcgtttga tgatatccag
    21661 ccccaacgta tggaaagtgg agatcatcag cccacgcgcc tctttgcgcc ccagcgtctg
    21721 ccctacacgc tctttcatct cgcgcgctgc tttattagta aaggtcaccg ccgcaatgtg
    21781 ccgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggtgt attgctcaat cttcgaacgg ggggaggatt
    21961 gtagcagaat cgcatttgaa tgtgaccgcg tgtaagccgc taaccggttg gctgcggatg
    22021 tttaaatcag agagagaaga ccgaaatcgg agttcatcgt gaatccatta gacttagaaa
    22081 atatcgggtc gtaacgttgt gttattactt tttcagagtt tactaaggag aggtttactc
    22141 gtggcgatta atttcagccc taaggttggt gaaatactgg aatgcaactt tggtaactat
    22201 cctgttagcc aaaatggacc gttcatcacc acgtattacg atggccgcat tcctcctgaa
    22261 atgataaaaa acaggttagt tgtcgtgctc aacggaaaaa ttaatggcaa cgcatgtatc
    22321 gtcgttccat tatcaaccac tcgcgaccat gacaagttaa atcgtggaat gcatgttgaa
    22381 atagccagta atattatcaa tgacctgcaa ttctttgatc agcagattcg ttgggccaaa
    22441 gccgacctgg tacaacaagt cagtcgaaac agattaaaca gagcaagaac ttacagaggg
    22501 tatcttaatc aatgtttgcc acatgagttg gttgcagaca tacagagggc ggtcatcaag
    22561 tcaatcaatg ccatatcgct cataaattga caataaaatc accatccccg ataatgactc
    22621 tgtagcccat agaggcccta agttttctcc ccttgaggga cctgcgaagc ccggattttc
    22681 cgggcttttg ccatatatgt gacccgtcct gactgacaat tacgttagca tctgcggatc
    22741 atgctaaaat cgccgcctga caattattca ctcaaggcac gatcatggca aaaacagcag
    22801 cagcactgca tatccttgta aaagaagaga aactggctct ggatcttctc gagcagatta
    22861 agaacggggc cgattttggc aagctggcga agaaacactc catttgccca tcaggcaaac
    22921 gcggcggtga tttaggtgaa ttccgccagg gtcagatggt tccggcgttc gataaagttg
    22981 tgttctcttg tccggtactg gagccgactg gcccgctgca tacccagttc ggttaccaca
    23041 tcattaaggt gctttaccgt aactaacagt aaggccttct ccaggagaag gccttgaact
    23101 ctgaatcgct attaacccgc aacagcaatg cgtttcatat ctgtcatata gccgcgcagt
    23161 ttcttaccta cctgctcaat cgcatggctg cgaatcgctt cgttcacatc acgcagttgc
    23221 gcgttatcta ccgcgccttc cggaatagct ttacccaggt cgcccggttg cagctctgcc
    23281 ataaacggtt tcagcaacgg cacacaagcg taagagaaca gatagttacc gtactcagcg
    23341 gtatcagaga taaccacgtt catttcgtac agacgcttac gggcgatggt gttggcaatc
    23401 agcggcagct cgtgcagtga ttcataatat gcagactctt caatgatgcc ggaatcgacc
    23461 atggtttcga acgccagttc aacgcccgct ttcaccatcg cgatcatcag tacgccttta
    23521 tcgaagtatt cctgctcgcc gattttgcct tcatactgcg gcgcggtttc aaacgcggtt
    23581 ttgccggttt cttcacgcca ggtcagcagt ttcttatcat cgttggccca gtccgccatc
    23641 ataccggaag agaattcgcc ggagatgatg tcatccatat gtttctggaa cagcggtgcc
    23701 atgatctctt tcagctgttc agacagcgcg taagcacgca gtttcgccgg gttagagaga
    23761 cggtccatca tcagggtgat gccgccctgt ttcagcgctt cggtgatggt ttcccagccg
    23821 aactgaatca gtttttctgc gtatgccggg tcggtgcctt cttccaccag cttgtcgaag
    23881 cacagcagcg agccagcctg caacataccg cacagaatgg tttgctcgcc catcaggtca
    23941 gatttcactt ccgcaacgaa ggacgattcc agcacgcccg cacggtgacc gccggtcgct
    24001 gccgcccagg ctttggcaat cgccatgcct tcaccttttg gatcgttttc cgggtgaacg
    24061 gcaatcagcg tcggtacacc gaatccacgt ttgtactctt cacgtacttc ggtgccaggg
    24121 catttcggcg caaccattac tacggtgatg tctttacgga tctgctcgcc cacttctacg
    24181 atgttgaaac catgcgagta acccagcgcc gcgccgtctt tcatcagtgg ctgtacggtg
    24241 cgcactacat cagagtgctg cttgtccggc gtcaagttaa ccaccagatc cgcctgcggg
    24301 atcagttctt cgtaagtacc aactttaaag ccgttttcgg tcgctttacg ccaggacgcg
    24361 cgcttctcgg caatcgcttc cttacgcagc gcgtaggaga tatccagacc agaatcacgc
    24421 atgttcaggc cctggttgag accctgtgcg ccacagccga cgatgaccac ttttttaccc
    24481 tgaaggtagc tcgcgccgtc agcgaattcg tcgcgaccca taaagcgaca tttgcccagc
    24541 tgtgccagct gctggcgcag gttcagtgta ttgaagtagt tagccatggt gattcctcgt
    24601 gatgttgtgc ttcttattgt tcggttcgct tttgcgagat tgaattcact atatgacagg
    24661 aaatttattg cggaaattga tatattcaca acgtcacatt gcaatttttg caacgtcaac
    24721 atcgagggct gtccctgtgg atttacgcga tctgaaaacc ttcctgcatc tggcggaaag
    24781 ccgccatttt ggccgcagcg cgcgggcgat gcacgttagc ccatccacgc tctcacggca
    24841 gattcagcgc ctggaagaag atctcggtca gccgctgttt gtgcgcgata accgcacggt
    24901 gacgctgact gaagcgggcg aagaactgcg cgttttcgcc cagcaaacgc tgttgcaata
    24961 tcagcaattg cgccacacca tcgaccagca aggaccgtcg ctctctggcg aattacatat
    25021 cttctgctcg gtgaccgctg cctacagcca tctgccgccg attctggatc gcttccgcgc
    25081 ggaacatccg tcggtggaga tcaaactcac cactggtgat gcggcagatg caatggaaaa
    25141 agtggtcacg ggagaagcgg atctggcgat tgccgggaaa ccggagacgc tacccggcgc
    25201 tgtggcgttt tcgatgctgg agaatctggc ggtagtgctg attgcgcccg cgctgccctg
    25261 cccggtgcgt aatcaggtct cggtagagaa gccggactgg tcaacggtac cgtttatcat
    25321 ggccgatcag gggccagtac gccgccgcat tgaactgtgg tttcgacgca ataaaatcag
    25381 caacccaatg atttacgcca cggttggcgg gcatgaagcg atggtatcga tggtggcgct
    25441 cggctgtggt gtggcgctgt tgccggaagt ggtgctggaa aacagcccgg agcctgtacg
    25501 caaccgggta atgattttag atcgcagcga tgagaaaacg ccgtttgaac tgggcgtgtg
    25561 tgcacaaaaa aagcggctgc atgagccgct gattgaggcg ttctggagga ttttgccgaa
    25621 ccacaaatga cgttgtcgcg cgagtaggcc tgataagcga agcgctatca ggcatttttc
    25681 cctaacccgc caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg caatcgtagc
    25741 ccagctcatt tagccgggtt tcgaaatccg gttcatggtc gccaagctcg aacgccgcca
    25801 gtacacgccc gtagtcggta ccgtgactgc gataatggaa cagagaaata ttccagtgcg
    25861 tacccagcgt gttgaggaag cgcagcagcg cacccggcga ttccggaaat tcgaagctgt
    25921 agagacgttc ctgcaacgga tgcgatggac gccccccaac catatagcgc acgtgtaact
    25981 tcgccatttc gtcgtcggag agatcaacca cgctgtagcc gccgtcgttg agcatctgca
    26041 aaatttcttt gcgctcttcg aggccgcggc tcaggcgcac gccgacaaag atgcaggcgt
    26101 ttttggcatc ggcaaaacgg tagttgaact cggtgaccga tcgcccgcca agcagttggc
    26161 agaatttgag gaagctgcct ttttcttccg gaatggtcac cgccaacaac gcttcacgct
    26221 gttcgcccag ttcgcagcgt tccgagacgt agcgcaggcc gtggaagttc acgttggcac
    26281 cggaaagaat atgcgccagc cgttcgccgc gaatgttgtg ctgggcgata tattttttca
    26341 ttcccgccag cgccagcgcg ccagagggtt ccgccaccgc gcgcacatct tcgaataaat
    26401 ccttcatcgc cgcacagatc gcatcactat cgacggtgat gatatcgtcg agatactcct
    26461 ggcacaaacg gaaggtttcg tcgccgatgc gttttaccgc aacgccttca gcaaacagcc
    26521 ccacgcgcgg cagatcaacc ggatgacccg catccagcgc tgctttcagg caggcggagt
    26581 cttccgcttc tacggcgatc actttgattt gcggcatcag ttgtttgatc agcaccgcca
    26641 cgcccgcagc cagaccgccg ccgccgactg gcacaaatac gcggtcgaga tgggcgtcct
    26701 gctggagcag ttccagcgcc agcgtgcctt gcccggcaat caccatcgga tgatcgaacg
    26761 gcggcaccca ggtgaacccc tgctgctgtg acagttcgat cgctttggct ttcgcttcat
    26821 caaagttcgc gccgtggagc agcacttcgc cgccgaagcc gcgcaccgcg tcgactttga
    26881 tatcggcggt ggcggttggc atgacgatca gcgccttcac gcctaaccgt gcggaagaaa
    26941 acgcgacgcc ctgcgcgtgg ttacccgcag aagcagtgat cacgccgtgc gctttttgtt
    27001 cttccgtcag gcccgccatc atggcgtatg cgccgcgcag cttaaagctg tgcactggct
    27061 ggcgatcttc gcgcttcacc agaatgacgt tatcaagacg cgacgacagt ttttccattt
    27121 tttgtagcgg cgtaacctgc gccgcctcgt aaaccggcgc gcgcagcact gctcttaaat
    27181 attcggcacc ttccggagca ccggacaggg gttgcgagtc agccattatt aaccccccag
    27241 tttcgattta tcgcgcaccg cgcctttgtc ggcgctggtt gccaggctgg cataagcacg
    27301 cagggcaaag gagacttgac gttcacgatt tttcggcgtc caggctttgt caccccgagc
    27361 ttcctgcgct tcacgacgcg ccgccagttc ggcatcgctt acctgtaact gaatgccacg
    27421 gttcggaatg tcgatggcga tcaggtcgcc gtcttcaatc aggccaatgc tgccgccgct
    27481 tgccgcttcc ggtgagacgt ggccgataga aaggccagag gtgccgccag agaaacgacc
    27541 gtcggtgatc agcgcacagg ctttgccgag gcccattgat ttcaggaagc tggttgggta
    27601 gagcatttcc tgcatgccag ggccgccttt cggaccttca tagcggatca ccaccacatc
    27661 gcccgcgaca accttgccgc cgagaatcgc ttctaccgcg tcgtcctggc tttcgtatac
    27721 tttcgccggg ccggtgaatt tgaggatgct gtcatcgacg cccgcggttt taacgatgca
    27781 gccgttttct gcgaaattac cgtagagcac tgccaggccg ccgtctttgc tgtacgcatg
    27841 ctccagcgag cgaatacagc catttgcgcg atcgtcgtcc agcgtatccc aacggcaatc
    27901 ctgcgagaat gcctgtgtag tacgaatgcc cgccgggcct gcacggaaca tattttttac
    27961 cgcgtcatcc tgggtcagca taacgtcgta ttgttccagc gtttgcggca acgtcaggcc
    28021 aagtacgttt ttcacatcac ggttcagtaa ccccgcacga tccagctcgc cgagaatacc
    28081 gataacacca ccagcacggt gaacatcttc catatggtat ttttgggtgc tcggcgcaac
    28141 tttgcataac tgcgggactt tacgcgaaag tttgtcgata tcactcatgg tgaagtcgat
    28201 ttccgcttcc tgcgccgccg ccagcaggtg aagtacggtg ttagtcgatc cacccatcgc
    28261 gatatccagc gtcatggcat tttcaaacgc cgctttgcta gcaatattgc gaggcagtgc
    28321 actttcgtcg ttttgctcgt agtaacgttt ggtcaattca acaatgcgtt taccagcatt
    28381 gaggaacagc tgcttacggt cggcgtgagt tgccagcagc gagccgttgc ccggctgcga
    28441 caggcccagc gcttcggtca ggcagttcat tgagttagcg gtaaacatcc cggagcagga
    28501 accgcaggtt gggcatgcgg aacgttcaac ctgatcgctc tgggagtcag aaactttcgg
    28561 gtctgccccc tggatcatcg catcaaccag atcgagcttg atgatctgat cggacagttt
    28621 ggttttcccg gcctccatcg ggccgccgga aacaaagatc accggaatat tcaggcgcag
    28681 agaggccatc agcatccccg gggtgatttt gtcacagtta gagatgcaga ccatggcgtc
    28741 ggcgcagtgg gcgttgacca tatactcaac ggaatcagcg atcagttcgc gagatggcag
    28801 tgaataaagc atccccccat ggcccatggc aatcccatca tccaccgcaa tggtgttgaa
    28861 ctctttggca acgccgccag ccgcttcaat ttgttcggcg accagtttac cgagatcgcg
    28921 cagatggacg tgacccggta caaattgggt gaacgagttc acaaccgcga taatcggctt
    28981 accgaaatcg gcgtcggtca ttccggtggc gcgccacagc gcacgagcac ccgccatatt
    29041 acgaccatga gtggtggtgg cggaacggta cttaggcata ctttatttac tcccagtgtc
    29101 tgtctcgtaa atgggacggt gcgtgccgtc ccattttttg tatttattga ttaacttgat
    29161 ctaaccagcc ccatttatct tcggtttcgc cggtgaagag gccgaagaag gcttgctgaa
    29221 tacgtttggt aaccgggcca caacggcctt cgccaacctg aataccgtct acgctgcgca
    29281 ctggcgtgat ttctgccgcc gtaccggaca taaacacttc atccgccagg tacagggatt
    29341 cgcgcgacag tacctgctca cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg
    29401 catcacgggt aatacccggc agcgcggagg aggtgaacgg tggggtgaac agcacaccat
    29461 ctttcacttc aaacaggttt tcgcctgcgc cttcagagat gtaaccattc acatccagcg
    29521 cgataccttc ctgataaccg tggcggcgtg cttcgctacc caccagcagg gaagagagat
    29581 agttaccacc ggctttcgcc gcggttggga tggtgtttgg tgctgcacgg ttccaggagg
    29641 aaaccatcgc atcgatcccc tgctccagcg cttctgcgcc cagatacgct ccccacggga
    29701 aagcggcgat aatcacatcg gttgagtatc ccgctggcgg gttaacgccc ataccaacat
    29761 caccgacgaa gatcagcgga cggatatagg cgctggtgag attgtttttg cggatcacgt
    29821 cacgacaagc ttccatcagc tcatcaatgc tctgcgaaac cgggaagcga tagattttgg
    29881 cggagtcatg cagacgctgc atatgctcac gatggcggaa tacaaccggt cctttgtgcg
    29941 agtcgtagca acggatgcct tcaaaaaccg aggtgccata gtgcagcgcg tgcgacatca
    30001 catgcacctt cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg taatcagctt
    30061 tcttcgtggt catttttata ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt
    30121 gtgctctggc agatggcaac gtgtgcgacg tccaccagtt tatttaactg actaaacagt
    30181 aagtcgaccg accgtgggct ggcaacggtc aattcgatat ttatattttg tgcatcgctg
    30241 gcggcggcca tattcattga gcagacgtgg aaaccacgat gacgcaccac gcgtaaaaca
    30301 cgttctaagg tttctggatt gaagcgagcc gatacattga cctgatgttg catcatgata
    30361 atttctccaa catttctgaa ttactggcgc caggcggcac cagcggccag acgttctcaa
    30421 gttcgtcgat tgagacatga agcaggtatg gcccatcact gttcagcatg gtgtcgagtg
    30481 ccgcttcaac ctggtcttta cgggtgatgt gttggccagg gatgccgaag gcgctggcta
    30541 acatgaggaa atcggggtta tcagtaaggg tggtttcgct gtatcgttcc tgaaaaaaca
    30601 gttgctgcca ttgtcgaacc atccctaacc gttggttatc gagtaagacg attttcaacg
    30661 gtaactgctt gcgttttacg gtgcccagct cttgcacatt catcatgaaa gagccgtcac
    30721 cggagataca gacaacggta tcgttcggtc gcgcgacttg tgcgccaacc gccgccggta
    30781 aaccaaaacc catggtgcct aagccgctgg aggtaatgaa attttccggg cgagtgtgtg
    30841 cgatgtgctg cgcggcccac atctgatgct gccccacatc tgtggtcaca acgcaatccg
    30901 caggtttacg atccgacagt tgttttaaca acaacggcgc gtagatagtg tcaccgggat
    30961 ggtcgtaacg ccaggcatgt tcatcactca gctgcgcgca gtattgctgc cagtcattga
    31021 tatttaatgg ctgctgtaat gctggtaaca gcgcatttaa atcaccctgt aatgccacat
    31081 gtgcctgacg cagcttgttc atttctgccg ggtcgatatc catatggata acactggcgt
    31141 gtggtgcgaa ggtgttcagt ttgccggtca cccggtcatc aaaacgtgca cccacggcga
    31201 tcagcaagtc gcactcctgc accgcgaagt tcgccgcttt ggtgccgtgc attcccagca
    31261 ttcccagata gtacgaataa tctgctgcaa ctgcgcccag ccctttcagc gtgcaggtgg
    31321 caggcatttt tgtggtagag agaaattctc gtaaagcagg aactgcctgc gccataccca
    31381 cgccaccacc aacgtacagc atcggttttt gcgcttttgc cagcatctgg cgcgcttgct
    31441 cgacttcggc atgggggaaa gtcacttcgt tttcaacggt ggtgaaccac ggttccaggt
    31501 cgccgctggc taactggata tcttttggga tatcgaccag aaccggacca ggacgacctg
    31561 agctggcaac gtcgaatgct tcagccatga tgcgcggcaa ctcttccagc gactgcacca
    31621 ggaagctgtg cttagtacag gctaacgaca atcccaggac atccacttcc tgaaatgcgt
    31681 cagtgccgat aaacggtgcg gacacttgac cggtgatggc aacaacaggg atagaatcta
    31741 acagtgcgtc cgcaagcccg gttatcaggt tggttgctcc cggaccagac gtggcgatac
    31801 atacgccagt tttgccggta gcacgagcat aaccgatagc cgccattgcc gcgccctgtt
    31861 catgtcggca cagcaagtgc tccacgccgc cgtcatacaa tgcatcgtaa accggcataa
    31921 ttgcgccacc cggataaccg aaaacggtgt tcacaccctg tgcccgcaac gcatgtacca
    31981 cccactgtgc gccattcata gttagttccc cgtcctgaat cttgagaaac agaattttgt
    32041 gctgttattc attgtctgct cctcggttat gtttttaagg tcaaaaaaaa cccccggacc
    32101 tttcggtgcg ggggtcttag ttcgttaagg cttgatctct aagcctttcc tcgtccaagt
    32161 gcagccccgc acggtgggat aataatcacc accacgctaa tcacgaccag gctaatcact
    32221 cgtagaaggg ctgtcatttt gtcttttctt gcatcttgtt cgaaggaatg cctaaagagt
    32281 taccatagat tttgcgaata gcacaagata ttttttggcc gatttttatg atgcctttaa
    32341 ta
//
LOCUS       DAZKFU020000024        31770 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZKFU020000024 DAZKFU020000000
VERSION     DAZKFU020000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.024"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            159..1727
                     /gene="cydA"
                     /locus_tag="ACHOQQ_RS04520"
                     /old_locus_tag="pgaptmp_000904"
     CDS             159..1727
                     /gene="cydA"
                     /locus_tag="ACHOQQ_RS04520"
                     /old_locus_tag="pgaptmp_000904"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGU4469196.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1743..2882
                     /gene="cydB"
                     /locus_tag="ACHOQQ_RS04525"
                     /old_locus_tag="pgaptmp_000905"
     CDS             1743..2882
                     /gene="cydB"
                     /locus_tag="ACHOQQ_RS04525"
                     /old_locus_tag="pgaptmp_000905"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4469197.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2897..3010
                     /gene="cydX"
                     /locus_tag="ACHOQQ_RS04530"
                     /old_locus_tag="pgaptmp_000906"
     CDS             2897..3010
                     /gene="cydX"
                     /locus_tag="ACHOQQ_RS04530"
                     /old_locus_tag="pgaptmp_000906"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGU4469198.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3010..3303
                     /gene="ybgE"
                     /locus_tag="ACHOQQ_RS04535"
                     /old_locus_tag="pgaptmp_000907"
     CDS             3010..3303
                     /gene="ybgE"
                     /locus_tag="ACHOQQ_RS04535"
                     /old_locus_tag="pgaptmp_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGU4469199.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     DLEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3453..3857
                     /gene="ybgC"
                     /locus_tag="ACHOQQ_RS04540"
                     /old_locus_tag="pgaptmp_000908"
     CDS             3453..3857
                     /gene="ybgC"
                     /locus_tag="ACHOQQ_RS04540"
                     /old_locus_tag="pgaptmp_000908"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4469200.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3854..4546
                     /gene="tolQ"
                     /locus_tag="ACHOQQ_RS04545"
                     /old_locus_tag="pgaptmp_000909"
     CDS             3854..4546
                     /gene="tolQ"
                     /locus_tag="ACHOQQ_RS04545"
                     /old_locus_tag="pgaptmp_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGU4469201.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4550..4978
                     /gene="tolR"
                     /locus_tag="ACHOQQ_RS04550"
                     /old_locus_tag="pgaptmp_000910"
     CDS             4550..4978
                     /gene="tolR"
                     /locus_tag="ACHOQQ_RS04550"
                     /old_locus_tag="pgaptmp_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGU4469202.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5043..6308
                     /gene="tolA"
                     /locus_tag="ACHOQQ_RS04555"
                     /old_locus_tag="pgaptmp_000911"
     CDS             5043..6308
                     /gene="tolA"
                     /locus_tag="ACHOQQ_RS04555"
                     /old_locus_tag="pgaptmp_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGU4469203.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6441..7733
                     /gene="tolB"
                     /locus_tag="ACHOQQ_RS04560"
                     /old_locus_tag="pgaptmp_000912"
     CDS             6441..7733
                     /gene="tolB"
                     /locus_tag="ACHOQQ_RS04560"
                     /old_locus_tag="pgaptmp_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGU4469204.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7768..8289
                     /gene="pal"
                     /locus_tag="ACHOQQ_RS04565"
                     /old_locus_tag="pgaptmp_000913"
     CDS             7768..8289
                     /gene="pal"
                     /locus_tag="ACHOQQ_RS04565"
                     /old_locus_tag="pgaptmp_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGU4469205.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8299..9090
                     /gene="cpoB"
                     /locus_tag="ACHOQQ_RS04570"
                     /old_locus_tag="pgaptmp_000914"
     CDS             8299..9090
                     /gene="cpoB"
                     /locus_tag="ACHOQQ_RS04570"
                     /old_locus_tag="pgaptmp_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGU4469206.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9255..9330
                     /locus_tag="ACHOQQ_RS04575"
                     /old_locus_tag="pgaptmp_000915"
     tRNA            9255..9330
                     /locus_tag="ACHOQQ_RS04575"
                     /old_locus_tag="pgaptmp_000915"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9288..9290,aa:Lys,seq:ttt)
     gene            9367..9442
                     /locus_tag="ACHOQQ_RS04580"
                     /old_locus_tag="pgaptmp_000916"
     tRNA            9367..9442
                     /locus_tag="ACHOQQ_RS04580"
                     /old_locus_tag="pgaptmp_000916"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9400..9402,aa:Val,seq:tac)
     gene            9445..9520
                     /locus_tag="ACHOQQ_RS04585"
                     /old_locus_tag="pgaptmp_000917"
     tRNA            9445..9520
                     /locus_tag="ACHOQQ_RS04585"
                     /old_locus_tag="pgaptmp_000917"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9478..9480,aa:Lys,seq:ttt)
     gene            9572..9647
                     /locus_tag="ACHOQQ_RS04590"
                     /old_locus_tag="pgaptmp_000918"
     tRNA            9572..9647
                     /locus_tag="ACHOQQ_RS04590"
                     /old_locus_tag="pgaptmp_000918"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9605..9607,aa:Val,seq:tac)
     gene            9651..9726
                     /locus_tag="ACHOQQ_RS04595"
                     /old_locus_tag="pgaptmp_000919"
     tRNA            9651..9726
                     /locus_tag="ACHOQQ_RS04595"
                     /old_locus_tag="pgaptmp_000919"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9684..9686,aa:Lys,seq:ttt)
     gene            9873..9948
                     /locus_tag="ACHOQQ_RS04600"
                     /old_locus_tag="pgaptmp_000920"
     tRNA            9873..9948
                     /locus_tag="ACHOQQ_RS04600"
                     /old_locus_tag="pgaptmp_000920"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9906..9908,aa:Lys,seq:ttt)
     gene            9982..10057
                     /locus_tag="ACHOQQ_RS04605"
                     /old_locus_tag="pgaptmp_000921"
     tRNA            9982..10057
                     /locus_tag="ACHOQQ_RS04605"
                     /old_locus_tag="pgaptmp_000921"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10015..10017,aa:Lys,seq:ttt)
     gene            10088..10213
                     /locus_tag="ACHOQQ_RS04610"
                     /old_locus_tag="pgaptmp_000922"
     ncRNA           10088..10213
                     /ncRNA_class="other"
                     /locus_tag="ACHOQQ_RS04610"
                     /old_locus_tag="pgaptmp_000922"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10491..11534
                     /gene="nadA"
                     /locus_tag="ACHOQQ_RS04615"
                     /old_locus_tag="pgaptmp_000923"
     CDS             10491..11534
                     /gene="nadA"
                     /locus_tag="ACHOQQ_RS04615"
                     /old_locus_tag="pgaptmp_000923"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGU4469207.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11572..12291
                     /gene="pnuC"
                     /locus_tag="ACHOQQ_RS04620"
                     /old_locus_tag="pgaptmp_000924"
     CDS             11572..12291
                     /gene="pnuC"
                     /locus_tag="ACHOQQ_RS04620"
                     /old_locus_tag="pgaptmp_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGU4469208.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALTWLAVCVIAIGLMTVFINPVFAFLTRVAVIIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12288..13229)
                     /gene="zitB"
                     /locus_tag="ACHOQQ_RS04625"
                     /old_locus_tag="pgaptmp_000925"
     CDS             complement(12288..13229)
                     /gene="zitB"
                     /locus_tag="ACHOQQ_RS04625"
                     /old_locus_tag="pgaptmp_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGU4469209.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13343..13723)
                     /gene="ybgS"
                     /locus_tag="ACHOQQ_RS04630"
                     /old_locus_tag="pgaptmp_000926"
     CDS             complement(13343..13723)
                     /gene="ybgS"
                     /locus_tag="ACHOQQ_RS04630"
                     /old_locus_tag="pgaptmp_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGU4469210.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14039..15091
                     /gene="aroG"
                     /locus_tag="ACHOQQ_RS04635"
                     /old_locus_tag="pgaptmp_000927"
     CDS             14039..15091
                     /gene="aroG"
                     /locus_tag="ACHOQQ_RS04635"
                     /old_locus_tag="pgaptmp_000927"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGU4469211.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15249..16001)
                     /gene="gpmA"
                     /locus_tag="ACHOQQ_RS04640"
                     /old_locus_tag="pgaptmp_000928"
     CDS             complement(15249..16001)
                     /gene="gpmA"
                     /locus_tag="ACHOQQ_RS04640"
                     /old_locus_tag="pgaptmp_000928"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGU4469212.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16203..17243)
                     /gene="galM"
                     /locus_tag="ACHOQQ_RS04645"
                     /old_locus_tag="pgaptmp_000929"
     CDS             complement(16203..17243)
                     /gene="galM"
                     /locus_tag="ACHOQQ_RS04645"
                     /old_locus_tag="pgaptmp_000929"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGU4469213.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALTSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHLWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            complement(17237..18385)
                     /gene="galK"
                     /locus_tag="ACHOQQ_RS04650"
                     /old_locus_tag="pgaptmp_000930"
     CDS             complement(17237..18385)
                     /gene="galK"
                     /locus_tag="ACHOQQ_RS04650"
                     /old_locus_tag="pgaptmp_000930"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGU4469214.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18389..19435)
                     /gene="galT"
                     /locus_tag="ACHOQQ_RS04655"
                     /old_locus_tag="pgaptmp_000931"
     CDS             complement(18389..19435)
                     /gene="galT"
                     /locus_tag="ACHOQQ_RS04655"
                     /old_locus_tag="pgaptmp_000931"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGU4469215.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19445..20461)
                     /gene="galE"
                     /locus_tag="ACHOQQ_RS04660"
                     /old_locus_tag="pgaptmp_000932"
     CDS             complement(19445..20461)
                     /gene="galE"
                     /locus_tag="ACHOQQ_RS04660"
                     /old_locus_tag="pgaptmp_000932"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGU4469216.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPMFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMCAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20723..22195)
                     /gene="modF"
                     /locus_tag="ACHOQQ_RS04665"
                     /old_locus_tag="pgaptmp_000933"
     CDS             complement(20723..22195)
                     /gene="modF"
                     /locus_tag="ACHOQQ_RS04665"
                     /old_locus_tag="pgaptmp_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGU4469217.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILCGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            complement(22263..23051)
                     /gene="modE"
                     /locus_tag="ACHOQQ_RS04670"
                     /old_locus_tag="pgaptmp_000934"
     CDS             complement(22263..23051)
                     /gene="modE"
                     /locus_tag="ACHOQQ_RS04670"
                     /old_locus_tag="pgaptmp_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGU4469218.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23180..23329
                     /gene="acrZ"
                     /locus_tag="ACHOQQ_RS04675"
                     /old_locus_tag="pgaptmp_000935"
     CDS             23180..23329
                     /gene="acrZ"
                     /locus_tag="ACHOQQ_RS04675"
                     /old_locus_tag="pgaptmp_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGU4469219.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23496..24269
                     /gene="modA"
                     /locus_tag="ACHOQQ_RS04680"
                     /old_locus_tag="pgaptmp_000936"
     CDS             23496..24269
                     /gene="modA"
                     /locus_tag="ACHOQQ_RS04680"
                     /old_locus_tag="pgaptmp_000936"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4469220.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24269..24958
                     /gene="modB"
                     /locus_tag="ACHOQQ_RS04685"
                     /old_locus_tag="pgaptmp_000937"
     CDS             24269..24958
                     /gene="modB"
                     /locus_tag="ACHOQQ_RS04685"
                     /old_locus_tag="pgaptmp_000937"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGU4469221.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24961..26019
                     /gene="modC"
                     /locus_tag="ACHOQQ_RS04690"
                     /old_locus_tag="pgaptmp_000938"
     CDS             24961..26019
                     /gene="modC"
                     /locus_tag="ACHOQQ_RS04690"
                     /old_locus_tag="pgaptmp_000938"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGU4469222.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26020..26838)
                     /gene="ybhA"
                     /locus_tag="ACHOQQ_RS04695"
                     /old_locus_tag="pgaptmp_000939"
     CDS             complement(26020..26838)
                     /gene="ybhA"
                     /locus_tag="ACHOQQ_RS04695"
                     /old_locus_tag="pgaptmp_000939"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGU4469223.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            26993..27988
                     /gene="pgl"
                     /locus_tag="ACHOQQ_RS04700"
                     /old_locus_tag="pgaptmp_000940"
     CDS             26993..27988
                     /gene="pgl"
                     /locus_tag="ACHOQQ_RS04700"
                     /old_locus_tag="pgaptmp_000940"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGU4469224.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28029..28982)
                     /gene="ybhD"
                     /locus_tag="ACHOQQ_RS04705"
                     /old_locus_tag="pgaptmp_000941"
     CDS             complement(28029..28982)
                     /gene="ybhD"
                     /locus_tag="ACHOQQ_RS04705"
                     /old_locus_tag="pgaptmp_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4469225.1"
                     /translation="MKHELSSMKAFVILAESNSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFS
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29166..30218
                     /gene="ybhH"
                     /locus_tag="ACHOQQ_RS04710"
                     /old_locus_tag="pgaptmp_000942"
     CDS             29166..30218
                     /gene="ybhH"
                     /locus_tag="ACHOQQ_RS04710"
                     /old_locus_tag="pgaptmp_000942"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGU4469226.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIIPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30294..31727
                     /gene="ybhI"
                     /locus_tag="ACHOQQ_RS04715"
                     /old_locus_tag="pgaptmp_000943"
     CDS             30294..31727
                     /gene="ybhI"
                     /locus_tag="ACHOQQ_RS04715"
                     /old_locus_tag="pgaptmp_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGU4469227.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT         7605 a         7779 c         8393 g         7993 t
ORIGIN      
        1 tcattaacat atttgtaact tttttatcat tgtcttttcc tctttagatg aaatggcaac
       61 gatagaggcg agcaaataat tttgtattgg ggcatgcgtg tgaccctttc taacggggtt
      121 cactctcgga gtcttcatgc gatgagcaag gagtcatgat gttagatata gtcgaactgt
      181 cgcgcttaca gtttgccttg accgcgatgt accacttcct ttttgtgcca ctgacgctcg
      241 gtatggcgtt cctgctggcc attatggaaa cggtctacgt cctttccggc aaacagattt
      301 ataaagatat gaccaagttc tggggcaagt tgtttggtat caacttcgct ctgggtgtgg
      361 ctaccggtct gaccatggag ttccagttcg ggactaactg gtcttactat tcccactatg
      421 taggggatat cttcggtgcg ccgctggcaa tcgaaggtct gatggccttc ttcctcgaat
      481 ccacctttgt aggtctgttc ttcttcggtt gggatcgtct gggtaaagtt cagcatatgt
      541 gtgtcacctg gctggtggcg ctcggttcaa acctgtccgc actgtggatt ctggttgcaa
      601 acggctggat gcaaaaccca atcgcgtccg atttcaactt tgaaactatg cgtatggaga
      661 tggtgagctt ctccgagctg gtgcttaacc cggttgctca ggtgaaattc gttcacactg
      721 tagcgtctgg ttatgttact ggcgcgatgt tcatcctcgg tatcagcgca tggtatatgc
      781 tgaaaggtcg tgacttcgcc ttcgctaaac gctcctttgc tatcgctgcc agcttcggta
      841 tggctgccgt tctgtctgtt attgttctgg gtgatgaatc cggctacgaa atgggcgacg
      901 tgcagaaaac caaactggct gctattgaag ccgagtggga aacgcaacct gcgcctgctg
      961 cctttactct gttcggcatt cctgatcagg aagaggagac gaacaaattt gcgatccaga
     1021 tcccttacgc gctgggcatc attgcaacgc gttccgtgga tactccagtt atcggcctga
     1081 aagaactgat ggtgcagcat gaagaacgca ttcgtaacgg gatgaaggcg tactctctgc
     1141 tcgaacagct gcgttctggt tctaccgacc aggcggttcg tgaccagttc aatagcatga
     1201 agaaagacct cggttatggt ctgctgctga aacgctatac gccaaacgtg gctgatgcga
     1261 ctgaagctca gattcaacag gcaaccaaag actccattcc gcgtgtagcg ccgctgtact
     1321 tcgcgttccg tatcatggtg gcgtgtggct tcctgctgct ggcaatcatc gcgctctctt
     1381 tctggagtgt tatccgcaac cgcattggcg agaaaaaatg gctgctgcgc gccgcgctgt
     1441 acggtattcc gctgccgtgg attgctgtag aagcgggctg gttcgtggct gaatatggcc
     1501 gccaaccgtg ggctatcggt gaagtgctgc cgacagctgt ggcgaactcg tcactgaccg
     1561 caggcgatct catcttctca atggtgctga tttgcggcct gtataccctg ttcctggtgg
     1621 cagaattgtt cttaatgttc aagtttgcac gcctcggccc aagcagcctg aaaaccggtc
     1681 gctatcactt tgagcagtct tccacgacta ctcagccggc acgctaagac aggagtcgtc
     1741 aaatgatcga ttatgaagta ttgcgtttta tctggtggct gctggttggc gttctgctga
     1801 ttggttttgc agtcactgat ggtttcgaca tgggggtggg catgctcacc cgtttcctcg
     1861 gtcgtaacga caccgagcgt cgaattatga ttaactccat cgcaccacac tgggacggta
     1921 accaggtttg gctgatcacc gcaggcggcg cactgtttgc tgcctggccg atggtctatg
     1981 ccgctgcgtt ttccggcttc tatgtggcga tgatcctcgt gctggcgtct ttgttcttcc
     2041 gtccggtcgg ttttgactac cgctccaaga ttgaagaaac ccgctggcgt aacatgtggg
     2101 actggggcat cttcattggt agcttcgttc cgccgttggt gattggtgta gcgttcggta
     2161 acctgttgca gggcgtaccg ttcaacgttg atgaatatct gcgtctgtac tacaccggta
     2221 acttcttcca gctgcttaac ccgttcggcc tgctggcagg cgtggtgagc gtagggatga
     2281 tcattactca gggcgcaacc tatctgcaaa tgcgtaccgt gggcgaactg cacctgcgta
     2341 cccgtgcaac ggctcaggtg gcggcactgg tgacactggt ctgtttcgca ctggctggcg
     2401 tatgggtgat gtacggtatc gatggttatg tcgtgaaatc gacaatggac cattacgcag
     2461 cctctaaccc gctgaataaa gaagtggttc gtgaagctgg cgcatggctg gttaacttca
     2521 acaacacgcc aattctgtgg gctattccgg cactgggtgt ggttctgccg ctgctgacca
     2581 tcctgactgc acgtatggat aaagccgcat gggcgtttgt gttctcctcc ctgacgctgg
     2641 cctgcatcat cctgactgct ggtattgcaa tgttcccgtt tgtgatgccg tccagcacca
     2701 tgatgaacgc aagcctgaca atgtgggatg caacttccag ccagctgacg cttaacgtca
     2761 tgacctgggt tgcggtggtt ctggtaccga tcattctgct ctacaccgcc tggtgttact
     2821 ggaaaatgtt cggtcgtatc accaaagaag atattgaacg taacacccac tctctgtact
     2881 aagtaaggag ctaaaaatgt ggtatttcgc atggattctg ggaacgcttc ttgcctgttc
     2941 gtttggggta atcaccgcgc tggcgcttga gcacgtcgaa tcaggcaaag ccggtcaaga
     3001 agacatctga tgagtaagat tatcgcgact ttgtatgcgg taatggacaa gcgccccctg
     3061 cgggcgcttt ccttcgtgat ggcgcttctg ttagcaggat gtatgttttg ggacccatca
     3121 cgtttcgccg cgaagaccag tgatctggaa atctggcatg gtttattgct gatgtgggcc
     3181 gtctgtgctg gtgtgattca cggcgtgggc tttcgtccgc agaaggttct ttggcaaggg
     3241 attttttgcc cattgcttgc cgatattgtt ctcattgtcg ggctgatttt cttcttcttt
     3301 taaatcagaa ttctctttaa aaaattatgg gccgcgccag gcccataaat ttttacgctc
     3361 ccttaacttg ccctcattcc caaacctcaa tcgcgcgcgt atagtagcag cgtttaaaag
     3421 ctctaacttt tgttgcatta ccgggatgta aagtgaatac aacgctgttt cgatggccgg
     3481 ttcgcgtcta ctatgaagat accgatgccg gcggtgtggt gtaccacgcc agttacgtcg
     3541 ctttttatga aagagcacgc acagagatgc tgcgtcatca tcacttcagt cagcaggcgc
     3601 tgatggctga acgcgttgcc tttgtggtac gtaaaatgac ggtggaatat tacgcacctg
     3661 cgcggctcga cgatatgctc gaaatacaga ctgaaataac atcaatgcgt ggcacctctt
     3721 tggttttcac gcaacgtatt gtcaacgccg agaatacttt gctgaatgaa gcagaggttc
     3781 tggttgtttg cgttgaccca ctcaaaatga agcctcgtgc gcttcccaag tctattgtcg
     3841 cggagtttaa gcagtgactg acatgaatat ccttgatttg ttcctgaagg ctagccttct
     3901 ggttaaactt atcatgttga ttttgattgg tttttcaatc gcatcctggg ccattattat
     3961 ccagcggacc cgtattctta acgcagcggc gcgcgaagcc gaagcgtttg aggataaatt
     4021 ctggtctgga atcgaactct ctcgcctcta tcaagagagc caggggaaac gggataatct
     4081 gaccggttcg gaacaaatct tttacagcgg gttcaaagaa tttgtgcgcc tgcatcgtgc
     4141 caatagccat gcgccggaag ccgtagtgga aggggcgtcg cgtgcgatgc gtatttccat
     4201 gaaccgtgaa cttgaaaatc tggaaacgca cattccgttc ctcggtacgg ttggctccat
     4261 cagcccgtat attggtctgt ttggtacggt ctgggggatc atgcacgcct ttatcgccct
     4321 cggggcggta aaacaagcaa cactgcaaat ggttgcgcca ggcatcgcag aagcgttgat
     4381 tgctacagca atcggtctgt tcgccgctat tccggcggta atggcttata accgtctcaa
     4441 ccagcgcgta aacaaactgg aactgaatta cgacaacttt atggaagagt ttaccgcgat
     4501 tctgcaccgc caggcgttta ccgttagcga gagcaacaag gggtaagcca tggccagagc
     4561 gcgtggacga ggtcgtcgcg atctcaagtc cgaaatcaac attgtaccgt tgctggacgt
     4621 actgctggtg ctgttgctga tctttatggc gacagcgccc atcatcaccc agagcgtgga
     4681 ggtcgatctg ccagacgcta ctgaatcaca ggcggtgagc agtaacgata atccgccagt
     4741 gattgttgaa gtgtctggta ttggtcagta caccgtggtg gttgagaaag atcgccttga
     4801 gcgtttacca ccagagcagg tggtggcgga agtgtccagc cgtttcaagg ccaacccgaa
     4861 aacggtcttt ctgatcggtg gcgcaaaaga tgtgccttac gatgaaataa ttaaagcact
     4921 gaacttgtta catagtgcgg gtgtgaaatc ggttggttta atgacgcagc ctatctaaac
     4981 atctgcgttt cccttgcttg aaagagagcg ggtaacaggc gaacagtttt tggaaaccga
     5041 gagtgtcaaa ggcaaccgaa caaaacgaca agctcaagcg ggcgataatt atttcagcag
     5101 tgctgcatgt catcttattt gcggcgctga tctggagttc gttcgatgag aatatagaag
     5161 cttcagccgg aggcggcggt ggttcgtcca tcgacgctgt catggttgat tcaggtgcgg
     5221 tagttgagca gtacaaacgc atgcaaagcc aggaatcaag cgcgaagcgt tctgatgagc
     5281 agcgcaagat gaaggaacag caggctgctg aagaactgcg tgagaaacaa gcggctgaac
     5341 aggaacgcct gaagcaactt gagaaagagc ggttagctgc tcaggaacag aaaaagcagg
     5401 ctgaagaagc cgcaaaacag gccgagttaa agcagaagca agcggaagag gcggcagcga
     5461 aagcagcggc agatgctaaa gcgaaggccg aagcggatgc taaagctgcg gaagaagcag
     5521 cgaagaaagc ggctgcagac gcgaagaaaa aagcagaagc agaagccgcc aaagccgcag
     5581 ccgaagcgca gaaaaaagcc gaggcagccg ctgcggcact gaagaagaaa gcggaagcgg
     5641 cagaagcagc tgcagctgaa gcaagaaaga aagcggcaac tgaagctgct gaaaaagcca
     5701 aggcagaagc tgagaagaaa gcggctgctg aaaaggctgc agctgataag aaagcggcag
     5761 cagagaaagc tgcagccgac aaaaaagcag cagaaaaagc ggctgctgaa aaggcagcag
     5821 ctgataagaa agcagcggca gaaaaagccg ccgcagacaa aaaagcggca gctgcaaaag
     5881 cagcagctga aaaagccgct gcagcaaaag ctgccgcaga ggcagatgat attttcggtg
     5941 agctaagctc tggtaagaat gcaccgaaaa cggggggagg ggcgaaaggg aacaatgctt
     6001 cgcctgccgg gagtggtaat actaaaaaca atggcgcatc aggggccgat atcaataact
     6061 atgccgggca gattaaatct gctatcgaaa gtaagttcta tgacgcatcg tcctatgcag
     6121 gcaaaacctg tacgctgcgc ataaaactgg cacccgatgg catgttactg gatatcaaac
     6181 ctgaaggtgg cgatcccgca ctttgtcagg ctgcgttggc agcagctaaa cttgcgaaga
     6241 tcccgaaacc accaagccag gcagtatatg aagtgttcaa aaacgcgcca ttggacttca
     6301 aaccgtaatc gcgatgttga ctgttccgac ggtcaacatc aggcaccggt tgccacggag
     6361 ttctggtagt tttgtgtatt ttagtttgtt aacattctgc taaattatcg tgggccatcg
     6421 gtccagataa gggagatatg atgaagcagg cattacgagt agcatttggt tttctcatac
     6481 tgtgggcatc agttctgcat gctgaagtcc gcattgtgat cgacagcggt gtagattccg
     6541 gtcgtcctat tggtgttgtt cctttccagt gggcgggtcc tggtgcggca cctgaagata
     6601 ttggcggtat cgttgctgct gacttgcgta acagcggtaa atttaatccg ttagatcgcg
     6661 ctcgtctgcc acagcagccg ggtagtgcgc aggaagtaca accagctgca tggtccgcgc
     6721 tgggtattga cgctgtagtt gtcggtcagg tcactccgaa tccggatggt tcttacaatg
     6781 ttgcttatca acttgttgac actggcggcg caccgggtac tgtacttgct cagaactcgt
     6841 acaaagtgaa caagcagtgg ctgcgttatg ctggtcatac cgccagtgat gaagtgtttg
     6901 aaaaactgac cggtattaaa ggtgcgttcc gtacccgtat tgcctacgtt gttcagacca
     6961 acggcggtca gttcccgtat gaactgcgcg tatctgacta tgacggttac aaccagtttg
     7021 tcgttcaccg ttcaccacag ccgctgatgt ccccggcgtg gtcaccagac ggttctaaac
     7081 tggcttatgt gaccttcgaa agcggtcgtt ccgcgctggt tatccaaacg ctggcaaacg
     7141 gcgctgtacg tcaggtggct tcattcccgc gtcacaacgg tgcgcctgca ttctcgccag
     7201 acggcagcaa actggcattc gccttgtcga aaaccggtag cctgaacctg tacgtaatgg
     7261 atttggcttc tggtcagatc cgccaggtga ctgatggtcg cagtaacaat accgaaccga
     7321 cctggttccc ggacagccag aacctggcat ttacttctga ccaggccggt cgtccacagg
     7381 tttataaagt gaatatcaac ggcggtgcgc cacaacgtat tacctgggaa ggttcgcaga
     7441 accaggatgc ggatgtcagc agcgacggta aatttatggt aatggtcagc tccaacggtg
     7501 ggcagcagca cattgccaaa caagatctgg caacgggagg cgtacaagtt ctgtcgtcca
     7561 cgttcctgga tgaaacgcca agtctggcac ctaacggcac tatggtaatc tacagctctt
     7621 ctcaggggat gggatccgtg ctgaatttgg tttctacaga tgggcgtttc aaagcgcgtc
     7681 ttccggcaac tgatggacag gtcaaattcc ctgcctggtc gccgtatctg tgataataat
     7741 taattgaata gtaaaggaat cattgaaatg caactgaaca aagtgctgaa agggctgatg
     7801 attgctctgc ctgttatggc aattgcggca tgttcttcca acaagaacgc cagcaatgac
     7861 ggcagcgaag gcatgctggg tgccggcact ggtatggatg cgaacggcgg caacggcaac
     7921 atgtcttccg aagagcaggc tcgtctgcaa atgcaacagc tgcagcagaa caacatcgtt
     7981 tacttcgatc tggacaagta cgatatccgt tctgacttcg ctcaaatgct ggatgcacat
     8041 gcaaacttcc tgcgtagcaa cccgtcttac aaagtcaccg tagaaggtca cgcggacgaa
     8101 cgtggtactc cggaatacaa catctccctg ggtgaacgtc gtgcgaacgc cgttaagatg
     8161 tacctgcagg gtaaaggcgt ttctgcagac cagatctcca tcgtttctta cggtaaagaa
     8221 aaacctgcag tactgggtca tgacgaagcg gcatactcca aaaaccgtcg tgcggtactg
     8281 gtttactaag agaattgcat gagcagtaac ttcagacatc aactattgag tctgtcgtta
     8341 ctggttggta tagcggcccc ctgggccgct tttgctcagg caccaatcag tagtgtcggc
     8401 tcaggctcgg tcgaagaccg cgtcactcaa cttgagcgta tttctaatgc tcacagccag
     8461 cttttaaccc aactccagca acaactctct gataatcaat ccgatattga ttccctgcgt
     8521 ggtcagattc aggaaaatca gtatcaactg aatcaggtcg tggagcggca aaagcagatc
     8581 ctgttgcaga tcgacagcct cagcagcggt ggtgcagcgg cgcaatcaac cagcggtgat
     8641 caaagcggtg cggcggcatc aacgacgccg acagctgatg ctggtactgc gaatgctggc
     8701 gcgccggtga aaagcggtga tgcaaacacg gattacaatg cagctattgc gctggtgcag
     8761 gataaatccc gccaggatga cgcaatggtg gcatttcaga atttcatcaa aaattaccct
     8821 gattcaactt acctgccaaa cgccaattat tggctgggtc agttaaacta caacaagggt
     8881 aaaaaagatg atgcggcgta ctattttgct tcggtagtga aaaactatcc gaagtcacca
     8941 aaggctgcag atgcgatgtt taaagtcggc gtcatcatgc aggacaaagg tgacaccgca
     9001 aaagcgaaag ccgtatacca gcaggttatc agtaaatacc ctggtactga tggcgctaaa
     9061 caggcgcaaa aacgtctgaa cgcgatgtaa tgcataacac acgaccagaa gtcgcattat
     9121 ttctggtcgt gtcgtgcgaa tcataagcag ttgagtgatc tacatcgaaa tttttgttgc
     9181 gctcaagtct gaaatcagta atatatgccg ccgttgccac gggatatcaa acaaaccgaa
     9241 agcaacgaaa aagtgggtcg ttagctcagt tggtagagca gttgactttt aatcaattgg
     9301 tcgcaggttc gaatcctgca cgacccacca actaacctag ttagttgtag tatccagcgt
     9361 agtatcgggt gattagctca gctgggagag cacctccctt acaaggaggg agtcggcggt
     9421 tcgatcccgt catcacccac caccgggtcg ttagctcagt tggtagagca gttgactttt
     9481 aatcaattgg tcgcaggttc gaatcctgca cgacccacca ctaattacgg tgggttcggt
     9541 ggaagtagtt tgtagtatcc agcgcagtat cgggtgatta gctcagctgg gagagcacct
     9601 cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccactc gggtcgttag
     9661 ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac
     9721 ccaccagttt taacatcaaa ctcagatgtt aagcgtgaag gataacgttg cgccagcaac
     9781 ggcccgtagg gcgagcgaag cgagtcatcc tgcacgaccc accaatctta aagattggcc
     9841 ccgagtaaaa atctttcagg taacacccgt atgggtcgtt agctcagttg gtagagcagt
     9901 tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccatc ctgaatgatt
     9961 aaagcagcat aatcccgcaa ggggtcgtta gctcagttgg tagagcagtt gacttttaat
    10021 caattggtcg caggttcgaa tcctgcacga cccaccaatg taaaaaagcg ccctaaaggc
    10081 gcttttttgc tatctgcgat actcaaagat tcgaacctgc agcaggtttg agttgagcgt
    10141 agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg cgagcgaagc gagtcatcct
    10201 gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatt caggcatctc
    10261 tcaatttcaa ttcttgaccc tgatgacata ctccgcgctt gctgcgtaaa caactttacg
    10321 tcggaggaat aagacgcaaa gtgtggcatt cggcatctca tacgtaattc gagtgacttt
    10381 tccccaccat tcgactatct tgtttagcat ataaaacaaa ttacaccgat aacagcgaat
    10441 atcacgctaa tgtcggtttt aacgttaagc ctgtaaaacg agatggtaag atgagcgtga
    10501 tgtttgatcc agacacggcg atttatcctt tccccccgaa gccgacgccg ttaagcattg
    10561 atgaaaaagc gtattaccgc gagaagataa aacgtctgct aaaagaacgt aatgcggtga
    10621 tggttgccca ctactatacc gatcccgaaa ttcaacaact ggcagaagaa accggtggct
    10681 gtatttctga ttctttggaa atggcgcgct tcggtgcaaa gcatcccgct tctacgttgt
    10741 tagtcgctgg ggtgagattt atgggagaaa ccgccaaaat tctcagtccg gaaaaaacaa
    10801 ttctgatgcc gacacttcag gctgagtgtt cactggatct cggctgccct gttgaagaat
    10861 ttaacgcatt ttgcgatgcc catcccgatc gtactgtcgt cgtctacgcc aacacttctg
    10921 ctgcggtaaa agcgcgcgca gattgggtgg taacttcaag cattgccgtc gaacttattg
    10981 atcatcttga tagtttgggt gaaaaaatca tctgggcacc cgacaaacat ctggggcgtt
    11041 acgtgcaaaa acagacgggc gcagacattt tatgctggca gggtgcttgc attgtgcatg
    11101 atgaatttaa gactcaggcg ttaacccgct tgcaagaaga atacccggat gctgccatac
    11161 tggtgcatcc agaatcacca caagctattg tcgatatggc ggatgcggtc ggttccacca
    11221 gtcaactgat cgctgctgca aaaacattgc cacatcagcg gcttatcgtg gcaaccgatc
    11281 ggggcatttt ctacaaaatg cagcaggcgg tgccagataa agagttactg gaagcaccaa
    11341 ccgcgggtga gggcgcaacc tgccgcagct gcgcgcattg tccgtggatg gccatgaatg
    11401 gccttcaggc tatcgctgag gcattagaac aggaaggaag taatcacgag gttcatgttg
    11461 atgagaggct gcgagagagg gcgctggtgc cgctcaatcg tatgctggat tttgcggcta
    11521 cactacgtgg ataacgaata ataaggcgta acgttacgct ttgggggaaa gatggatttt
    11581 tttagtgtgc agaatatcct ggtacatata ccaatagggg caggcggtta tgatctctca
    11641 tggatcgaag cggtaggcac gatcgccggg ttgctgtgta ttggccttgc cagtctggag
    11701 aagatcagca actacttctt tggcctgatc aacgtcacct tgtttggcat tattttcttt
    11761 cagattcagc tgtatgccag cctgctatta caggtgtttt tctttgccgc gaatatttac
    11821 ggctggtatg cgtggtcgcg acaaaccagt cagaacgagg cggagttgaa aattcgctgg
    11881 ttaccattgc cgaaggcatt aacctggctg gcggtttgtg tcattgctat tggtctgatg
    11941 acggtattta tcaatccggt gtttgcattt ttgacccgcg tggcagtcat catcatgcaa
    12001 gcattaggat tacaggttgt gatgcctgaa ctgcaaccgg acgctttccc gttctgggat
    12061 tcatgcatga tggtgttatc tatcgtggca atgattctga tgacgcgtaa gtatgtggaa
    12121 aactggctgt tgtgggtgat tattaacgtg attagcgtcg ttatttttgc acttcagggc
    12181 gtttacgcca tgtctctgga gtacatcatc ctgaccttta ttgcgctcaa cggcagccgg
    12241 atgtggatca acagcgcacg tgaaagaggc tcacgcgcgc tgtcccatta atggtgatga
    12301 tgtgaatggc cagacacccc ttcattcagg tggcagtctg gcccatgaca aggttgatat
    12361 tccatctgaa tggtggcgtg ctcaatctga tagtgatcca tcaggtagtg ttggatctga
    12421 tccaacaagg catcgtgatc gtgtggcggg atcacctgca catgcaacgt catcaccggc
    12481 ttctcgccta ccatccatac atgcacatgg tgcacattgc ggacttccgg gatttcccgg
    12541 cacatacgac gcttcagttc agcgatatcc agcgataccg gtgcaccctc aagtaattca
    12601 ttcacgctat ctttcaacag tcgccacgcg ctacgcagaa ccagaagcga caccagtatc
    12661 gagagaatgg ggtcagcagg tgtccagccg gtccagataa taatcaacgc ggcaataata
    12721 gccccaaccg aacccagcag gtccccgagc acatgcagtg ccgctgctcg tacgttgagg
    12781 tttttctctt cgctgccgtg atgaagtaac cagaaagaaa gtatatttgc cagcaaccca
    12841 gccacggcaa ttgccatcat catgccgccc tcgaccggac gcggcgtgcg gaagcgttct
    12901 atcgcctccc agacaatcaa aatggtaatt accacgaggg cgatggcatt cacaaacgcc
    12961 gcgagcgtgg tcagtcttag ccaaccgaaa gtgtggcgaa tggtgggagg acgacgtgaa
    13021 aattgcacgg cgagtagggc gaaaagcagg gcggcagtat cggtcaacat atgacccgca
    13081 tcggccagca aagccagaga accagaaaga aaaccaccaa tgacttctac cagcataaac
    13141 ccggcagtca cgccgaaagc atacaacaag cgacgagcat tattatcttc tggcaggtgt
    13201 gaggacgtgt gtgagtgtga gtgcgccatg acgagtcatc ccttaattat tctcacttaa
    13261 ctttatgaca tcatactgct tttagaaggg aaaaattaaa agggagagac tccgctctcc
    13321 cattattggc tattttgcag ggttactgcg tggtaccgtc ggttttggta tcgacatcat
    13381 tattgatgcc atcaccggtt tgtacctttt tattgatatc cggacagcga ccatctttgc
    13441 acatggtgtt cttgtgctct tcatctttgg tcatgccgtc attgttcatt gaagaaccat
    13501 ccggatgcag cattgtgccg ccagaacccg tatttacccc gttattgtcg acgttatttg
    13561 gcgcgacatt ttcacgggcg tcaggggcta cctggcccgc atcagcggcg gcgtttgcct
    13621 ggccgttatt agtttgcgct ccgctatcgg cagccagtgc ggcaccgctg gcaaggctta
    13681 gagtggcagt cagaaataat gtggccagtt ttgtcatttt cataggatgc tcctgttatg
    13741 gtcgttatgt cggataacct cttccaacag tgcatttgca ggtgaatata aggcattggt
    13801 ttaagatttc agccaggcaa agaaatccag cagagaatct tgaaataatt aacaaacaaa
    13861 ggagttacag ttagaaattg taggagagat ctcgtttttc gcgacaatct ggcgtttttc
    13921 ttgctaattc caggattaat ccgttcatag tgtaaaaccc cgtttacaca ttctgacgga
    13981 agatatagat tggaggtatt gcattcacta agataagtat ggcaacactg gaacagacat
    14041 gaattatcag aacgacgatt tacgcatcaa agaaatcaaa gagttacttc ctcctgtcgc
    14101 attgctggaa aaattccccg ctactgaaaa tgccgctaat acggttgccc atgcccgaaa
    14161 agcgatccat aaaatcctga aagggaatga tgatcgcctg ttagtggtga ttggtccgtg
    14221 ctcaattcat gatcctgtag cggctaaaga gtacgccact cgcttgctgg cgctgcgtga
    14281 agagctgaaa gatgagctgg aaatcgtaat gcgcgtctat tttgaaaagc cgcgcaccac
    14341 ggtgggctgg aaagggctga ttaacgatcc gcatatggac aatagcttcc agatcaacga
    14401 cggtttgcgt attgcccgta aattgctgct tgatattaac gacagcggtc tgccagcggc
    14461 gggtgagttt ctcgatatga tcaccccaca atatctcgct gacctgatga gctggggcgc
    14521 aattggcgca cgtaccaccg aatcgcaggt gcaccgcgaa ctggcatcag ggctttcttg
    14581 tccggtcggc ttcaaaaatg gcaccgacgg tacgattaaa gtggctatcg atgccattaa
    14641 tgccgccggt gcgccgcact gcttcctgtc cgtaacgaaa tgggggcatt cggcgattgt
    14701 gaataccagc ggtaacggcg attgccatat cattctgcgc ggcggtaaag agcctaacta
    14761 cagcgcgaag cacgttgctg aagtgaaaga agggctgaac aaagcaggcc tgccagcaca
    14821 ggtgatgatc gatttcagcc atgctaactc gtcaaaacaa ttcaaaaagc agatggatgt
    14881 ttgtgctgac gtttgccagc agattgccgg tggcgaaaag gccattattg gcgtgatggt
    14941 ggaaagtcat ctggttgaag gcaatcagag cctggagagc ggggagccgc tggcctacgg
    15001 taagagcatc actgatgcct gcattggctg ggaagatacc gatgctctgt tacgtcaact
    15061 ggcgaatgca gtaaaagcgc gtcgcgggta aggtttaatt gtcggatgca ccgccagagt
    15121 ggcgtatccg atgaatcacc gtaggcctga taagacgcgc agcgccgcat caggcaatgt
    15181 gctccattgt tagcaacaaa aaagccgact cacttgcagt cggctttctc attttaaacg
    15241 aatgacgttt acttcgcttt accctggtta gcaaccgccg ctgctttcgc tgcgatctcg
    15301 tcagcattac ccagataata gcgtttcagc ggtttgaaat tctcgtcgaa ctcatacacc
    15361 agcggcacgc cagtcgggat attcagctca agaatctctt cttcgctcat gttatcaaga
    15421 tatttcacca gcgcacgcag ggagttaccg tgtgcggcga tgattacgcg ctcaccgctc
    15481 ttcatacgcg gtagaatagt ttcattccag taagggatca cgcggtcaat ggtcagcgcc
    15541 aggctttcag tcagcggcag ctctttctcg ctcagtttcg cgtaacgcgg atcgtgaccc
    15601 ggataacgct catcatcttt agtcagttcc ggcggagtca cggcaaaacc acgacgccat
    15661 tgtttcacct gctcgtcgcc atacttttcg gcggtttccg ctttgttcag accctgcaac
    15721 gcaccgtagt gacgttcgtt cagtttccag gatttctcaa cgggcagcca tgcctgatcc
    15781 agttcgtcca gcacattcca cagggtatgg atagcgcgtt tcagcacaga agtgtaagca
    15841 aagtcaaagc tgtaaccttc ctctttcagc agcttacctg ctgcttttgc ttcgcttacg
    15901 cctttctcag aaagatccac gtcgtaccaa ccggtgaaac ggttttcttt gttccactga
    15961 ctttcgccat gacgaaccag aaccagctta gttacagcca tatacttact cctcaaatca
    16021 tcttttaatg ataataattc tcattatatt gccgtgacga agcaacagca atgcttacgc
    16081 ataaccatag cgaaaatagt ggcgcagtgt aaggttgttg tgaatattga gttgcaaata
    16141 tgtcggtgtt tgctggtgat ttgaacaata tgagataaag ccctcatgac gagggcttaa
    16201 acttactcag caataaactg atattccgtc aggctggaat actcttcacc aggatgcagg
    16261 aagcagtccg gttgcggcca ttcagggtgg ttcgggctgt ccggcagaaa ctcgctttcc
    16321 agagccagcc cttgccagtc ggcgtaaggt tcggttcccc gcgacggtgt gccgccgagg
    16381 aaattgccgg agtaaaattg cagagccgga gcagtggtgt agaccttcag ctgcaatttt
    16441 tcatctgctg accagagatg cgccgccact ttcttgccat cgcctttggc ctgtaacaag
    16501 aacgcgtgat cgtaaccttt tactttgcgc tgatcgtcgt cggcaagaaa ctcactggcg
    16561 atgattttgg cgctgcggaa atcaaaagac gttccggcga cagatttcag gccgtcgtgc
    16621 ggaatgccgc cttcatcaac cggcagatat tcgtccgcca gaatctgcaa cttgtgattg
    16681 cgcacgtcag actgctcgcc gtcaagattg aaatagacgt gattagtcat attcaccggg
    16741 caaggtttat caactgtggc gcgataagta atggagatac ggttatcgtc ggtcagacga
    16801 tattgcaccg tcgcgccgag attacccggg aagccctgat cgccatcatc tgaagtcagg
    16861 gcaaacagca cctgacgatc gttctggttc acaatctgcc agcgacgttt gtcgaaccct
    16921 tccgggccgc cgtgcagctg gttaacgccc tgacttggcg aaagcgtcac ggtttcaccg
    16981 tcaaaggtat aacggctatt ggcgatgcgg ttggcataac gaccaataga ggcccccaga
    17041 aacgcggcct gatcctgata gcattccggg ctggcacagc cgagcagcgc ctcgcggacg
    17101 ctgccatcgg aaagcggaat acgggctgaa agtaaagtcg caccccagtc catcagcgtg
    17161 actaccatcc ctgcgttgtt acgcaaagtt aacagtcggt acggctgacc atcgggtgcc
    17221 agtgcgggag tttcgttcag cactgtcctg ctccttgtga tggtttacaa acgtaaaaag
    17281 tctctttaat acctgttttt gcttcatatt gttcagcgac agcttgctgt acggcaggca
    17341 ccagctcttc cgggatcagc gcgacgatac agccgccaaa tccgccgccg gtcatgcgta
    17401 cgccaccttt gtcgccaatc acagctttga cgatttctac cagagtgtca atttgcggca
    17461 cggtgatttc gaaatcatcg cgcatagagg catgagactc cgccatcaac tcgcccatac
    17521 gtttcaggtc gccttgctcc agcgcgctgg cagcttcaac ggtgcgggcg ttttcagtca
    17581 ggatatgacg cacgcgtttc gccacgattg ggtccagctc atgtgcaaca gcgttgaact
    17641 cttcaatggt gacatcgcgc agagctggct gctggaagaa acgcgcaccg gtttcgcact
    17701 gttcacgacg ggtgttgtat tcgctgccaa ccagggtacg tttgaagtta ctgttgatga
    17761 tgacgacagc cacacctttc ggcatggaaa ctgctttggt ccccagtgag cgacagtcaa
    17821 tcagcaaggc atgatctttc ttgccgagtg cggaaattag ctgatccatg atcccgcagt
    17881 tacagccaac aaactggttt tctgcttcct gaccgttaag cgcgatttgt gcgccgtcca
    17941 gcggcagatg ataaagctgc tgcaatacgg ttccgaccgc gacttccagt gaagcggaag
    18001 aacttaaccc ggcaccctgc ggcacattgc cgctgatcac catgtccacg ccgccgaagc
    18061 tgttgttacg cagttgcaga tgtttcacca cgccacgaac gtagttcgcc cattgatagt
    18121 tttcatgcgc gacaatgggc gcatcgaggg aaaactcgtc gagctgattt tcataatcgg
    18181 ctgccattac gcgaacttta cggtcatcgc gtggcgcaca actgatcacg gtttgataat
    18241 caatcgcgca gggcagaacg aaaccgtcgt tgtagtcggt gtgttcacca atcaaattca
    18301 cgcggccagg cgcctgaatg gtgtgagtgg cagggtagcc aaatgcgttg gcaaacagag
    18361 attgcgtttt ttctttcaga ctcatttctt acactccgga ttcgcgaaaa tggatatcgc
    18421 tgactgcgcg caaacgctct gctgcctgtt ctgcggtcag gtctcgctgg gtctctgcca
    18481 gcatttcata accaaccata aatttacgta cggtggcgga gcgcaacaga ggcggataaa
    18541 agtgcgcgtg cagctgccag tgttgattct cttcgccatt aaatggcgcg ccgtgccagc
    18601 ccatagagta ggggaaggag cactggaaga ggttgtcata acgactggtc agctttttca
    18661 acgccagcgc cagatcgctg cgctgggcgt cggtcaaatc ggtgatccgt aaaacgtggg
    18721 ctttgggcag cagtagcgtt tcgaacggcc aggcagccca gtaaggcact acggctaacc
    18781 agtgttcggt ttcaacaacg gtacggctac cgtctgccag ctcgcgctga acataatcca
    18841 ccagcattgg tgatttctgc tcggcaaaat attctttttg caggcggtct tcgcgctcag
    18901 cttcgttagg caggaagcta tttgcccaaa cctgtccgtg cggatgcggg ttagagcagc
    18961 ccatcgccgc gcctttattt tcaaagacct gcacccatgg gtacgttttc cccagttctg
    19021 cggtttgctc ctgccaggtt ttgacgattt ccgtcaatgc cgcaacactg agctctggca
    19081 gcgttttact gtgatccggt gaaaagcaga tcacccggct ggtaccgcgc gcgctctggc
    19141 aacgcatcaa aggatcgttg ctttctggcg catctggcgt gtcagacatc aaggccgcaa
    19201 agtcattagt gaaaacgtaa gtcccggtgt aatcggggtt tttatcgcct gtcacccgca
    19261 cattacctgc gcagaggaag caatctggat cgtgcgcagg taacacctgt ttggctggcg
    19321 tttcctgcgc cccctgccag gggcgcttag cgcggtgcgg tgaaaccaga atccattgcc
    19381 cggtgagcgg gttgtagcgg cgatgtggat gatcaacggg attaaattgc gtcatggtcg
    19441 ttccttaatc gggatatccc tgtggatggc gtgactgcca gtgccaggtg tcctgcgcca
    19501 tttcatcgag tgtgcgcgtt acgcgccagt tcagttcacg gtcggctttg ctggcgtccg
    19561 cccagtaggc cggaaggtcg ccctcgcgac gcggtgcaaa atgatagtta accggtttgc
    19621 cgcaggcttt gctgaaggca ttaaccacgt ccagcacgct gctgcctacg ccagcaccga
    19681 ggttgtagat gtgtacgcct ggcttgttcg ccagtttttc catcgccacg acgtgaccgt
    19741 ccgccagatc cattacgtgg atgtaatcgc gtacgccagt accgtcttcg gtcggataat
    19801 cgttaccaaa aatcgccagc gagtcgcgac ggcctacagc aacctgggcg atgtatggca
    19861 tcaggttatt cggaatgcct tgcggatctt cgcccatatc gcccgacgga tgcgcgccaa
    19921 ccgggttgaa gtagcgcagc agggcaatgc tccagtccgg ctgggctttt tgcagatcgg
    19981 tgaggatctg ttccaccatc agcttgcttt tgccgtaagg gctttgcggt gtgccggtcg
    20041 ggaagctttc aacgtatgga attttgggct gatcgccata aacggtggcg gaggagctaa
    20101 aaataaagtt tttgacgtta gcggcgcaca tggcgctaat caagcgcaga gtaccgttga
    20161 cattgttgtc gtaatattcc agcggttttt gtaccgattc gccaacggct ttcagcccgg
    20221 cgaagtggat cacggtgtcg atagcgtgat cgtgcaggat ctcggtcatc aacgcttcgt
    20281 tacggatatc gccttcaaca aacatcggat gtttgccgcc taaacgctcg ataacaggca
    20341 gtacgctgcg cttactgtta cagaggttat caagaatgat gacatcatga ccgttttgca
    20401 gtaattgcac acaggtatga cttccaatgt aaccgctacc accggtgacc agaactctca
    20461 taattcgctc cattaggctt atggtatgaa ataaccatag cataacaaag atgcgaaaag
    20521 tgtgacatgg aataaattag tggaatcgtt tacacaagaa tttagccgtt ttttatgcgc
    20581 gattaagtga ttataaaaca gagggattgt gaatgattgc gctttttatc tgaaaaaaag
    20641 acgcagtttc atgcctggat gcgtctgacc gtgtgccgga taaagcgttt acgccgcatc
    20701 cggcacagtc agcattaccg acttagttta tttttgtcag cgcataatga tagaggccac
    20761 cgtccggcac gaactccaga cgatgggtaa tacaagctgg cgcatcttca gcatggtgcg
    20821 aaacaaacaa caattgcgtt tcaccttcgc taatcagcac atcaacaaaa cggcgaataa
    20881 gctggcgatt cagcggatca agcccctgca atggttcgtc gagaataagc aacgtcggat
    20941 gtttcaccag tgcgcggacg atcagcgcca gacgctgctg tccccaggaa agactatgga
    21001 acggagcgtc agccgtgcgt ttatcaatgc cgagaatatc cagccactgc tgcaccagtt
    21061 tttgctggcg atccgaaacg gcctgataaa tgccaatcga atcaaaatag ccacaaagaa
    21121 tcacattacg cacagtagta ctgacccggt aatccagatg caaactactg ctgacgtaac
    21181 cgatatgctt tttgatatcc cagatggttt cgccgctgcc acgacgtcgt ccgaaaagcg
    21241 tcaaatcgtt gctgtaacct tgcggatgat cgccagtaat caggcttaat aacgtcgatt
    21301 ttcccgcacc atttggcccg acaatttgcc agtgctcgcc aggattcacc tgccagctaa
    21361 ggttattaag aatggggcga tcattataag aaaccacgcc attattcagc acaatgcgcg
    21421 gttcgttggt gggtaaggcg tgacgtgctg aaggttcatc cggctccggc agttgcacac
    21481 cttcaagctg ttcactatgc gccagttgcg cgacgagtgc ttgttggagc agttcctctt
    21541 tagcgccagt ttccgctaac gtgcaatccg ccagcacgcc agcaaactgg acaaactccg
    21601 ggatctcatc gaagcgattg agcaccagta ccagagtaat accggactga tgtaacgagg
    21661 cgagtcgctc agccagctgc tgacgtgagg caacatccag gccatcgaac ggctcatcaa
    21721 gaatcaacaa gtcaggctcc gacatcagcg cctgacacag cagggttttt cgcgtctcgc
    21781 cagtggaaag gtatttaaag cgtcggtcga ggagggcggt aataccgaac tgctgtgcca
    21841 gttgcatgca gcgcggtgca tcctttactt catcctgaat aatctcagcc gtagtgcgtc
    21901 cggtgtcatc ttcgccaggg ccgagcatat cggtgttatt ccgctgccat tcgtcgctga
    21961 cgagcttttg caattgctcg aaggagagac gagtgatgtg ggaaaactgg ctttgccgtt
    22021 cacctttcaa aagcggaagt tcccccgcca gcgcgcgggc cagggccgat ttcccgcttc
    22081 cattcgaacc gacaaacgcc caactatcac ccgcgtttaa cgttagctga ggcaattgca
    22141 gcgtttttgt gtcgctaaga cgaaacgtgc cttgcaaaat ttgcaacgat gacattttat
    22201 atcccatttt gtgcagcgat tactgacagg gatacgtgtt tcataacaaa ttgtcaacac
    22261 gcttagcaca gcgtggcgat aatcacgctg tcggcattaa agtaggccgt gacattctgt
    22321 ccttgctgaa gagaagtcgc ttcatttacc ggcactgtgg cgcacagtgt ttgcccgtcc
    22381 ggtagcgcca ttaatacttc gcactgctct gcgccgcgct caatatgact aataataccc
    22441 ggtaattggt tgtcagcgtt ttgcgcgacc gcttcgtcct gagtaatgcc tacccacggc
    22501 gcttttagca atatcaacac ttctttgcct tcatccagcc ccagacgcgc gccgctttgt
    22561 gcggtaattg cgactttcag gcgtgttttt ccatcagcca gtaagacatc aacatgctgt
    22621 tgaacgtcat catgatcgcg ggcggtgatg gtaccgaacc actggttacg ggcgctggtt
    22681 tgcagtgaaa aacgtgagat cgcggccagc aggctgttca gcggcagggc gtcatcgtca
    22741 cttaacacat caaaggcttt ttgctggatt tgcgccagta agtcatagag ctgaatcagt
    22801 cgctgaccat agcgggtcag tactgcgccg ccgccacctt taccgcctgt tgcgcgctcg
    22861 accagaatat gctcacttaa ctgattcatc tcgttaatgg catcccaggc gcttttatag
    22921 ctgataccgg catctttcgc tccctggcta atggaaccgg aaagcgcaat gtgttttagt
    22981 agcgaaatgc ggcgcgggtc ggcgaataat ttttgttgga gcttaagggt gagaaggatt
    23041 tcggcctgca taacaatgtc ctggcaaaag tcttattgtg acggaaaacg aacgccacgc
    23101 aaagctgacc gcacaaaagg ggagtgcttt tctgtgctta gcggttagaa tagtctcatg
    23161 actatatctg gagttgacca tgttagagtt attaaaaagt ctggtattcg ccgtaatcat
    23221 ggtacctgtc gtgatggcca tcatcctggg tctgatttac ggtcttggtg aagtattcaa
    23281 catcttttct ggtgttggta aaaaagacca gcctggacaa aatcattgat tccctgaatg
    23341 cccgcttagt cgggcatttt ctttttctca acttcctgct tttcctgccg atattttttc
    23401 ttatctacct cacaaaggtt agcaataact gctgggaaaa ttccgagtta gtcgttatat
    23461 tgtcgcctac ataacgttac attaaggggt taccaatggc tcgtaaatgg ttgaacttgt
    23521 ttgctggggc ggcactctct ttcgctgttg ctggcaatgc actggcagat gaagggaaaa
    23581 tcacggtgtt cgccgccgca tcactgacta acgcaatgca ggacattgct acgcagtata
    23641 aaaaagagaa aggcgtggat gtggtttctt ctttcgcttc gtcatctact ctcgcccgtc
    23701 agattgaagc gggtgcccct gcggatctgt ttatttctgc cgatcagaaa tggatggatt
    23761 atgcggttga taaaaaagcg atcgatacag ctacgcgtca gacactgctc ggcaatagcc
    23821 tggtcgttgt agcaccgaaa gccagcgagc agaaagattt taccatcgac agcaaaacca
    23881 actggacttc actgctgaat ggcggtcgcc tggcggttgg cgatccggaa catgttcccg
    23941 ctggcattta tgcaaaagaa gcactgcaaa aactgggcgc atgggatacg ctctccccga
    24001 aactggctcc ggcggaagat gttcgtggcg cgctggcgtt ggtcgaacgc aatgaagcgc
    24061 cgttgggcat tgtttacggt tctgacgcag ttgccagcaa aggggtaaaa gtggttgcca
    24121 ccttcccgga agattcacat aaaaaagtgg aatatccggt tgctgttgtg gaagggcata
    24181 acaatgcgac agtgaaagca ttttatgatt atctgaaggg accgcaggct gcggaaatct
    24241 ttaaacgtta cggatttaca accaagtaat gatactgacc gatccagaat ggcaggcagt
    24301 tttattaagc ctgaaagttt cttccctggc tgtgctgttt agcctgccgt ttgggatctt
    24361 ttttgcctgg ttactggtgc gttgcacgtt tccgggcaaa gctctgctcg acagcgtact
    24421 gcatctaccg ctggtgttac cgcccgtggt cgtcggttac ttattattag tttcgatggg
    24481 acggcgcgga tttatcggtg aacgtttgta tgactggttt ggcattacct tcgcctttag
    24541 ctggcgcggc gcggttctcg ctgccgccgt catgtcgttt ccgctgatgg tgcgggcaat
    24601 tcgtctggcg ctggaaggag ttgatgtcaa actggaacag gccgcaagaa cactgggtgc
    24661 cgggcgctgg cgcgttttct ttactatcac gttaccgctg acattaccgg gcattattgt
    24721 tggtacggta ctggcttttg ctcgttctct cggtgagttt ggtgcaacca tcacctttgt
    24781 gtcgaacatt cctggtgaaa cgcgaaccat tccttctgcc atgtataccc tgattcagac
    24841 ccccggcggg gaaagtggcg cagcgagact gtgcattatt tctattgcgc tggcgatgat
    24901 ctccctgttg atttcagaat ggctggccag aatcagccgt gaacgggcgg ggcgctaatc
    24961 atgctggaac tgaatttttc ccagacgttg ggcaaccatt gcctgactat taatgaaacg
    25021 ctgcccgcca atggcatcac tgctatcttt ggcgtctccg gtgccggaaa aacttcgctg
    25081 attaacgcca tcagtggact gacgcgcccg caaaaaggga ggattgtcct caatggtcgg
    25141 gtactaaatg atgccgagaa aggtatctgc ctgacgccgg aaaagcgtcg cgttggctat
    25201 gtttttcagg atgcgcggct gttcccgcat tacaaagtgc gtggcaatct gcgctacggc
    25261 atggcgaaaa gcatggtcga tcagttcgat aagctggtgg cgcttttagg cattgaaccg
    25321 ttgcttgacc gtttaccagg cagcctgtcc ggaggcgaaa aacagcgcgt agcgattggg
    25381 cgggctttgc tgacggcacc agaattgctg ttgctggatg aaccgctggc gtcactggat
    25441 attccgcgta aacgcgaact gttgccttat ctgcaacgtc tgacgcggga aatcaacatc
    25501 ccgatgttgt atgtcagcca ttcgctggat gagatcctcc atctggcaga cagagtgatg
    25561 gtactggaaa acggtcaggt gaaagccttt ggcgcgctgg aagaagtctg gggtagtagc
    25621 gtgatgaatc cgtggctgcc gaaagagcaa caaagtagca ttctgaaagt gacggtactt
    25681 gagcatcatc cgcattacgc gatgactgcg ctggcgctgg gcgatcagca tttgtgggtc
    25741 aataagctgg acgaaccgct gcaagctgcg ctacgtatcc gcattcaggc ttccgatgtt
    25801 tctctggttt tacaaccgcc gcagcaaacc agcattcgta acgtattgcg ggcaaaagtt
    25861 gttaatagtt atgacgacaa cggccaggtg gaagtggaac tggaggtcgg cggtaaaacg
    25921 ctgtgggcgc gtatcagccc gtgggccagg gatgaactgg cgatcaaacc tggcctgtgg
    25981 ctgtacgcgc aaattaaaag tgtgtcgata accgcctgat taaatcaggt ggctataaat
    26041 gaactgggca atgctgtcgg tggtgttatc accaatcaca atgttggcgc gcgcttttac
    26101 cgcgtcatcg gcgttgccca tcgccacgcc tgtaccagcg gcctccagca tactgatatc
    26161 attaaagtta tcgccgaacg ccacgacgtt ttccatcgac cagccttgcg cctcaaccca
    26221 tttcgtcaaa cgtttacctt tgctgttgcc gccgcgtgca atatcaacct gatcgtgcca
    26281 ggaccattca cactccagtc ccagttcatg ttcgacatgc ttaccaaaat gctgcaattg
    26341 cggcaggtca tcgtgcgtga gggcgaactt ccatacggcg ttaacttgtt gcgccgtttc
    26401 agccagagaa gcgacttgtg tgaaagtcgg acgctgttcc ggcggcaggg tttgcgccca
    26461 gttagacgtg cgaatgacat gcccggtcgg gtgctcataa accatcgcgt catcgacata
    26521 catcagaccg tgaatgtggt gttcattcag catctcaatg agttgcaggg ctttgttaac
    26581 aggcattgga tcggcttcaa gcacagtttt tgcatgataa tcatacaaat aggtgccatt
    26641 acagcaaata gcaggtgtat ccagcgccag cgcctgataa aaaggatgaa tagcgacgtg
    26701 atggcgacct gtgacgatga ttaattgata acctgcttcg cgagcgcggg ccagggcttc
    26761 tatcgatgaa ggtagcaggg tctttttcgg ggtcaataag gtgccgtcta agtcgagagc
    26821 aatcacgcgt gtggtcatgg cgtattccag attaagatta agaattttct tctgcgcgaa
    26881 tggtacaccg ataccactat cggacaaaat tctgcgtttt aattcagcat tcaccgccaa
    26941 aagcgactaa ttttagctgt tacagtcagt tgctaaatgc aaaggagcat tcatgaagca
    27001 aacagtttat atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga
    27061 aggcgcactg acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt
    27121 cagcccggac aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta
    27181 tcgtatcgcc ccggacgatg gcgcactgac ctttgccgca gagtctgcgc tgccgggtag
    27241 tccgacgcat atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc
    27301 gggtaacgtg agcgtaacgc gtctggaaga tggcctgcca gttggcgtcg tcgatgtggt
    27361 cgaggggctg gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt
    27421 tccggcatta aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt
    27481 ggcgcaggac cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt
    27541 ccatccaaac gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg
    27601 ggaactgaaa gatccgcacg gcaatatcga atgtgtccag acgctggata tgatgccgga
    27661 aaacttctcc gacacccgtt gggcggctga tattcatatt accccggatg gtcgtcactt
    27721 atatgcctgt gaccgtaccg ccagcctgat tactgtcttc agcgtttcgg aagatggcag
    27781 cgtgttgagt aaagagggct tccagccaac tgaaacccag ccgcgcggct ttaatatcga
    27841 tcaccgtggt aagtatctga ttgccgccgg gcaaaaatct caccatatct cggtatacga
    27901 aattgttggt gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc
    27961 gatgtgggtg gtggttaacg cacactaacc gctgatttac ccggcgcagt ctctcctgcg
    28021 ccggtgtatt aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac
    28081 aaattgtctt tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttcagctgcc
    28141 ggagaaagca cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc
    28201 accagtggga taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc
    28261 gctgaaatcc ccagtcctgc ctccaccagt cctaatgacg tcgaaagatg acgcacttcg
    28321 taaaaccagt ccagcttcca gggcttgtcg gctagctgtt gttctatcaa cagtcggttg
    28381 ccgctggagg agcgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc
    28441 tgctttttgg ccaatggatg gtcacgccga caggccagca cgaacggctc gttgaccagt
    28501 gggctaaaat caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg
    28561 ttacatagca ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata
    28621 ttgggataca gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc
    28681 ggaatacacg ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa
    28741 agcgtttcat cgaatttctt tattaattcc cgcgcctctg ggagcaacct ttttcctgct
    28801 tttgttaacg taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc
    28861 atctttttta ttcttcgtgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg
    28921 ttaaatgaat tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc
    28981 attattactc cgggaaatgg aagcgacgat tttgggtggc tggccgttaa aaattttaac
    29041 tgcatttagc caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta
    29101 gagatgcttt gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca
    29161 ttattatgaa aaaaataccc tgcgtgatga tgcgaggtgg aacctcgagg ggcgcgttcc
    29221 tgttagcgga acatttaccc gaagatcaaa cgcagcgcga taaaatattg atggcaatta
    29281 tgggttccgg taacgatctg gaaattgacg gtattggcgg cggtaatcca ctgaccagta
    29341 aagtcgccat tattagccgt tccagcgatc cgcgtgctga tgttgattat ctgtttgctc
    29401 aagttatcgt ccatgagcaa cgtgtcgata ccacgcctaa ctgcggcaat atgctgtctg
    29461 gcgttggggc attcgccatt gaaaatggtt tgattgcagc gacttcgcca gttacccgcg
    29521 tacgtatccg caacgtcaat acgggtacgt tcatcgaagc tgatgtgcaa acgccaaatg
    29581 gtgttgtcga gtacgagggt agtgccagaa ttgacggcgt accgggtact gccgcaccgg
    29641 ttgcgctcac tttcctgaat gccgctggaa ccaaaaccgg aaaagttttc ccgactgata
    29701 atcagattga ttattttgac gatgtcccgg tgacctgtat cgatatggcg atgccagtcg
    29761 tcattattcc ggctgaatat ctgggaaaaa caggctatga attaccggcg gaactggatg
    29821 ccgacaaagc attattagcc cgcattgaat ctatccgtct acaagcgggt aaagcaatgg
    29881 gcttaggtga tgtcagtaat atggttatcc ctaaacctgt gcttatttct ccagcgcaga
    29941 aaggcggggc aattaatgtg cgttatttta tgccgcattc ttgccatcgc gcgttggcga
    30001 taaccggtgc tattgctatt tccagtagtt gtgcattgga aggcaccgtc acccgacaaa
    30061 tcatcccttc tgtaggatat ggcaatatca atattgaaca ccccagtggt gcgctcgacg
    30121 ttcatttaag taatgaaggt caggatgcca cgacgttacg cgcatctgtt attcggacga
    30181 ccagaaaaat attttccggt gaagtttatc ttccctgaaa agattcgttg tcaggataag
    30241 gacaatcaat aaaggacttc tgtatgagtc atacagaaag aacaggattt taaatgaata
    30301 agaaatcgtt atggaagcta attctgatat tagcgatccc atgtattatt ggctttatgc
    30361 cagctccggc aggattaagc gaactggcgt gggtgctttt tggtatttac ctagcggcca
    30421 ttgtggggct ggttatcaag cctttcccgg aacctgtcgt actgttaatt gccgttgctg
    30481 cctccatggt ggtggtcggt aacttatccg acggtgcgtt taaaaccacc gccgtattaa
    30541 gcggttactc ttcaggtact acctggctgg tgttctcggc gtttacctta agcgccgcat
    30601 ttgtgaccac cggtttaggt aaacgtattg cctatctgct gattggtaaa atcggtaaca
    30661 ccacgctggg tctgggttac gttacggttt tcctcgatct ggtactggct ccggcaacac
    30721 cgtctaacac cgcgcgtgcg ggcggcattg tgttaccgat catcaacagc gtggcggtgg
    30781 ctttggggtc cgaaccggaa aaaagtccgc gtcgtgtcgg acattacctg atgatgtcca
    30841 tttacatggt caccaaaacc accagctata tgttctttac cgcaatggcg gggaacattc
    30901 tggcgctgaa aatgatcaac gacattctgc acctgcaaat tagctggggg ggatgggcgc
    30961 tggccgccgg attgccgggc atcattatgc tgctggttac tccgctggtg atttacacca
    31021 tgtatccgcc agaaattaag aaggtggata acaaaaccat cgctaaagcg ggccttgccg
    31081 aactgggacc gatgaaaatc cgcgaaaaaa tgctgctcgg tgtctttgtg ctggcgctgc
    31141 tgggctggat tttcagtaag tctctggggg ttgatgaatc caccgtggca atcgttgtta
    31201 tggcaaccat gctgctgctg ggtattgtta cctgggaaga cgtggttaaa aataaaggcg
    31261 gctggaatac tttaatttgg tacggcggta ttatcggctt aagctcctta ttatcgaaag
    31321 ttaaattctt cgaatggtta gctgaagtct ttaaaaataa cctggcattt gatggtcacg
    31381 gtaacgttgc tttcttcgtt attattttcc tcagcatcat cgtgcgttat ttcttcgctt
    31441 ccggtagtgc ctatatcgtt gctatgttac ctgtatttgc catgctggcg aacgtctccg
    31501 gcgcaccgtt aatgttaacc gcgctggcac tgttgttctc caactcctat ggcggcatgg
    31561 ttactcacta tggcggcgcg gcaggtccgg tcatctttgg cgtgggttac aacgatatta
    31621 aatcctggtg gttggtcggt gcggtactga cgatattaac cttcctggtg catatcaccc
    31681 tcggcgtgtg gtggtggaat atgctgatcg gctggaacat gctgtaaata tactcgtcat
    31741 acttcaagtt gcatgtgctg cgtctgcgtt
//
LOCUS       DAZKFU020000025        31709 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZKFU020000025 DAZKFU020000000
VERSION     DAZKFU020000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.025"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            374..499
                     /gene="timP"
                     /locus_tag="ACHOQQ_RS04720"
                     /old_locus_tag="pgaptmp_000944"
     CDS             374..499
                     /gene="timP"
                     /locus_tag="ACHOQQ_RS04720"
                     /old_locus_tag="pgaptmp_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGU4469228.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNLSLSGNNLLVVNNLQNSR"
     gene            complement(531..1307)
                     /gene="sseB"
                     /locus_tag="ACHOQQ_RS04725"
                     /old_locus_tag="pgaptmp_000945"
     CDS             complement(531..1307)
                     /gene="sseB"
                     /locus_tag="ACHOQQ_RS04725"
                     /old_locus_tag="pgaptmp_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGU4469229.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDVEEIIQAAGSVATDTLPADEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1450..2733)
                     /gene="pepB"
                     /locus_tag="ACHOQQ_RS04730"
                     /old_locus_tag="pgaptmp_000946"
     CDS             complement(1450..2733)
                     /gene="pepB"
                     /locus_tag="ACHOQQ_RS04730"
                     /old_locus_tag="pgaptmp_000946"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGU4469230.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVFACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2792..2992)
                     /gene="iscX"
                     /locus_tag="ACHOQQ_RS04735"
                     /old_locus_tag="pgaptmp_000947"
     CDS             complement(2792..2992)
                     /gene="iscX"
                     /locus_tag="ACHOQQ_RS04735"
                     /old_locus_tag="pgaptmp_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGU4469231.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3004..3339)
                     /gene="fdx"
                     /locus_tag="ACHOQQ_RS04740"
                     /old_locus_tag="pgaptmp_000948"
     CDS             complement(3004..3339)
                     /gene="fdx"
                     /locus_tag="ACHOQQ_RS04740"
                     /old_locus_tag="pgaptmp_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGU4469232.1"
                     /translation="MPKIVILPHQELCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3341..5191)
                     /gene="hscA"
                     /locus_tag="ACHOQQ_RS04745"
                     /old_locus_tag="pgaptmp_000949"
     CDS             complement(3341..5191)
                     /gene="hscA"
                     /locus_tag="ACHOQQ_RS04745"
                     /old_locus_tag="pgaptmp_000949"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGU4469233.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5208..5723)
                     /gene="hscB"
                     /locus_tag="ACHOQQ_RS04750"
                     /old_locus_tag="pgaptmp_000950"
     CDS             complement(5208..5723)
                     /gene="hscB"
                     /locus_tag="ACHOQQ_RS04750"
                     /old_locus_tag="pgaptmp_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGU4469234.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5819..6142)
                     /gene="iscA"
                     /locus_tag="ACHOQQ_RS04755"
                     /old_locus_tag="pgaptmp_000951"
     CDS             complement(5819..6142)
                     /gene="iscA"
                     /locus_tag="ACHOQQ_RS04755"
                     /old_locus_tag="pgaptmp_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGU4469235.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6159..6545)
                     /gene="iscU"
                     /locus_tag="ACHOQQ_RS04760"
                     /old_locus_tag="pgaptmp_000952"
     CDS             complement(6159..6545)
                     /gene="iscU"
                     /locus_tag="ACHOQQ_RS04760"
                     /old_locus_tag="pgaptmp_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGU4469236.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6573..7787)
                     /gene="iscS"
                     /locus_tag="ACHOQQ_RS04765"
                     /old_locus_tag="pgaptmp_000953"
     CDS             complement(6573..7787)
                     /gene="iscS"
                     /locus_tag="ACHOQQ_RS04765"
                     /old_locus_tag="pgaptmp_000953"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGU4469237.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7899..8387)
                     /gene="iscR"
                     /locus_tag="ACHOQQ_RS04770"
                     /old_locus_tag="pgaptmp_000954"
     CDS             complement(7899..8387)
                     /gene="iscR"
                     /locus_tag="ACHOQQ_RS04770"
                     /old_locus_tag="pgaptmp_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGU4469238.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8658..9398)
                     /gene="trmJ"
                     /locus_tag="ACHOQQ_RS04775"
                     /old_locus_tag="pgaptmp_000955"
     CDS             complement(8658..9398)
                     /gene="trmJ"
                     /locus_tag="ACHOQQ_RS04775"
                     /old_locus_tag="pgaptmp_000955"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGU4469239.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9517..10320
                     /gene="suhB"
                     /locus_tag="ACHOQQ_RS04780"
                     /old_locus_tag="pgaptmp_000956"
     CDS             9517..10320
                     /gene="suhB"
                     /locus_tag="ACHOQQ_RS04780"
                     /old_locus_tag="pgaptmp_000956"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGU4469240.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            10465..11319
                     /locus_tag="ACHOQQ_RS04785"
                     /old_locus_tag="pgaptmp_000957"
     CDS             10465..11319
                     /locus_tag="ACHOQQ_RS04785"
                     /old_locus_tag="pgaptmp_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4469241.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKNGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            11510..12790
                     /gene="csiE"
                     /locus_tag="ACHOQQ_RS04790"
                     /old_locus_tag="pgaptmp_000958"
     CDS             11510..12790
                     /gene="csiE"
                     /locus_tag="ACHOQQ_RS04790"
                     /old_locus_tag="pgaptmp_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGU4469242.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVCFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12782..13921)
                     /locus_tag="ACHOQQ_RS04795"
                     /old_locus_tag="pgaptmp_000959"
     CDS             complement(12782..13921)
                     /locus_tag="ACHOQQ_RS04795"
                     /old_locus_tag="pgaptmp_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGU4469243.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14081..14971)
                     /gene="hcaR"
                     /locus_tag="ACHOQQ_RS04800"
                     /old_locus_tag="pgaptmp_000960"
     CDS             complement(14081..14971)
                     /gene="hcaR"
                     /locus_tag="ACHOQQ_RS04800"
                     /old_locus_tag="pgaptmp_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGU4469244.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            15107..16468
                     /gene="hcaE"
                     /locus_tag="ACHOQQ_RS04805"
                     /old_locus_tag="pgaptmp_000961"
     CDS             15107..16468
                     /gene="hcaE"
                     /locus_tag="ACHOQQ_RS04805"
                     /old_locus_tag="pgaptmp_000961"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGU4469245.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16465..16983
                     /gene="hcaF"
                     /locus_tag="ACHOQQ_RS04810"
                     /old_locus_tag="pgaptmp_000962"
     CDS             16465..16983
                     /gene="hcaF"
                     /locus_tag="ACHOQQ_RS04810"
                     /old_locus_tag="pgaptmp_000962"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGU4469246.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            16983..17303
                     /locus_tag="ACHOQQ_RS04815"
                     /old_locus_tag="pgaptmp_000963"
     CDS             16983..17303
                     /locus_tag="ACHOQQ_RS04815"
                     /old_locus_tag="pgaptmp_000963"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGU4469247.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17300..18112
                     /gene="hcaB"
                     /locus_tag="ACHOQQ_RS04820"
                     /old_locus_tag="pgaptmp_000964"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17300..18112
                     /gene="hcaB"
                     /locus_tag="ACHOQQ_RS04820"
                     /old_locus_tag="pgaptmp_000964"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGU4469248.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAAIGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18122..19324
                     /gene="hcaD"
                     /locus_tag="ACHOQQ_RS04825"
                     /old_locus_tag="pgaptmp_000965"
     CDS             18122..19324
                     /gene="hcaD"
                     /locus_tag="ACHOQQ_RS04825"
                     /old_locus_tag="pgaptmp_000965"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGU4469249.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            19421..19843
                     /locus_tag="ACHOQQ_RS04830"
                     /old_locus_tag="pgaptmp_000966"
     CDS             19421..19843
                     /locus_tag="ACHOQQ_RS04830"
                     /old_locus_tag="pgaptmp_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4469250.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19891..20763)
                     /locus_tag="ACHOQQ_RS04835"
                     /old_locus_tag="pgaptmp_000967"
     CDS             complement(19891..20763)
                     /locus_tag="ACHOQQ_RS04835"
                     /old_locus_tag="pgaptmp_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU4469251.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNHFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20775..21869)
                     /locus_tag="ACHOQQ_RS04840"
                     /old_locus_tag="pgaptmp_000968"
     CDS             complement(20775..21869)
                     /locus_tag="ACHOQQ_RS04840"
                     /old_locus_tag="pgaptmp_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4469252.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMEHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(21902..22900)
                     /locus_tag="ACHOQQ_RS04845"
                     /old_locus_tag="pgaptmp_000969"
     CDS             complement(21902..22900)
                     /locus_tag="ACHOQQ_RS04845"
                     /old_locus_tag="pgaptmp_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4469253.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22924..24435)
                     /locus_tag="ACHOQQ_RS04850"
                     /old_locus_tag="pgaptmp_000970"
     CDS             complement(22924..24435)
                     /locus_tag="ACHOQQ_RS04850"
                     /old_locus_tag="pgaptmp_000970"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4469254.1"
                     /translation="MFTATEAVPVAKMVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSGLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEIRALRHKP
                     KLDDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITHPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASRILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(24458..25441)
                     /locus_tag="ACHOQQ_RS04855"
                     /old_locus_tag="pgaptmp_000971"
     CDS             complement(24458..25441)
                     /locus_tag="ACHOQQ_RS04855"
                     /old_locus_tag="pgaptmp_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4469255.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQFVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEITQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(25538..28819)
                     /locus_tag="ACHOQQ_RS04860"
                     /old_locus_tag="pgaptmp_000972"
     CDS             complement(25538..28819)
                     /locus_tag="ACHOQQ_RS04860"
                     /old_locus_tag="pgaptmp_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGU4469256.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDSLDYRCNLALAMLEYNR
                     ADFPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASKLL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKALGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            28937..30130
                     /locus_tag="ACHOQQ_RS04865"
                     /old_locus_tag="pgaptmp_000973"
     CDS             28937..30130
                     /locus_tag="ACHOQQ_RS04865"
                     /old_locus_tag="pgaptmp_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGU4469257.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30193..31446)
                     /gene="glyA"
                     /locus_tag="ACHOQQ_RS04870"
                     /old_locus_tag="pgaptmp_000974"
     CDS             complement(30193..31446)
                     /gene="glyA"
                     /locus_tag="ACHOQQ_RS04870"
                     /old_locus_tag="pgaptmp_000974"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGU4469258.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
BASE COUNT         7435 a         8811 c         7965 g         7498 t
ORIGIN      
        1 ccggtaaaag atattactta aacatgtaaa ttcactttct ttaagcaaca aaaaaccgcc
       61 aaaatcaggc ggttatttgt tgccggtttg gttcgcggcc tctccagcag attgacttgt
      121 gttgcatgag caacgcaggt gcttcacagc agagcaatat tcgccagcaa ctctcttttt
      181 gtcaagcaaa agagagtaat tattgtttat ttagcgtatt atcgacaccg gccctttccg
      241 ccgtctcgca aacgggcgct ggctttagga aaggatgttc cgtggccgta aatgcaggtg
      301 tttcacagcg cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg
      361 catggaccgc acaatgaaga tacggtgctt ttgtatcgta cttattgttt ctggtgcgct
      421 gttaaccgag gtaaataata acctgagtct ctccggcaac aatttactgg tggttaacaa
      481 ccttcagaac agcaggtaag cccgaatgcc gccctttggg gcggcatatt ttagattatc
      541 cggttctgtt taaagtcacg caaaaaacca ccccaacgac gttcatagaa tggcgcaata
      601 tgttcggtaa taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt
      661 ggttcatcgg caggtaatgt atcggtcgct acacttcccg ccgcctgaat aatttcttcg
      721 acatcaccat cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta
      781 attgaacata taaaagcacg cttcaccggc ttaatagttt taaataaggt ggtgagtgaa
      841 tcaatcattt gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc
      901 aggatttcct ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc
      961 ggcataaatt ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc
     1021 atctcaaaca atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt
     1081 tgaagtgctt ctaacgaggt gaaaaaagga ataacgctgg tgccgtcttc tttttcccag
     1141 tgctgtaaat caagcgcgct atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc
     1201 acccagacgg tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt
     1261 gctgcttttt ccagcaggtc ttcaagttca ttttttgttt cggacataag aaccacaatt
     1321 cattcaacgt tcggggcaca agtgcctgat gcgccatact tatcaggcct acaagggagt
     1381 cgcaatgtat tgaatttgca atactttgta ggccggataa ggcgtttacg ccgcatccgg
     1441 catattagtt tacgccgtta acagattagc aatcgtgcgc acaccaagtc ccgtagcacc
     1501 cgcagaccac tgttcaaccg gcgctttacg gtaagtcgcc gagcagtcga tatgcagcca
     1561 gccttgctga tagttctcaa caaagtgcga caggaagccc gccgccgtgc tcgcgcctgc
     1621 cggatacgcc gcgcttccgg tattgttcag ttcggcaaag ttagacggca gctggctgcg
     1681 gtggaactcc gccagcggca gacgccagaa tggttcgttc tcttgtgagg cactcgccag
     1741 caaacgaccg gccagcgcat cgtcaaaact gaacagcgca tgataatcat tacccagcgc
     1801 ggttttcgcc gccccggtga gggtcgccgc atcaatgatc agttccggtt tctgcgcact
     1861 ggcatcaatc aggccatcgg ccagcaccag acgcccttcg gcatcagtgt tcatcacttc
     1921 aactttttta ccgttgcgat aggtgatgat atcgcccagc ttgaacgcat tgccgctaat
     1981 caggttatcc gcacagcaga ggaacagctt cacgcgcttg ttcagtccgc gcgtaatggc
     2041 aaatgccagc gccccggtaa ccgttgccgc gccgcccatg tccgacttca tcgagtccat
     2101 aaacgcagtc tgtttgatgc tgtagccgcc ggagtcaaaa gtgatacctt tacctaccag
     2161 gcacgcgaac actggcgctt ctttatcgcc agttgggttg taatccagcg ccagcaatac
     2221 cggagaacgt tctgaaccgc gtccgacggt gtgcagcccc atataacctt gctcacgcag
     2281 atcttcgcct ttggtgatcc gataagtcac acgatcgccc gcgacgttgc tgatcagatc
     2341 aacagcacgc tgtgccagtt gcgatggtcc caattcttct gccggtgcgt tgatggtgtc
     2401 acgcacccag tcgatgatca tcaggcggtt atccagttcc tggcgctggg catcgtccag
     2461 atccggccac tccactttac gcgtgccttt cggggctttg taaccttgcc agaatgccca
     2521 gcagcgatcc gcatcccagc cttcaccgct tagctgaaca tgcttgatgc ccagaccgtc
     2581 aatcttgcgc gccgcacgct ggatcagccc cagatcgtct gccccgttca aatgcagagt
     2641 aatgccatca ttattaatgc tgtaagttgc tttttctccc cagcgcgcgt cggcaggttg
     2701 ggtagagagg gtaattttca tcgcttctgt cattttagtt atccttattc gtttaacagc
     2761 aaaacgggct acccccaggc agcccgttca ttcactcggc ttcgtccagc cagactaaca
     2821 aaatcgcttc gaggattttc tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat
     2881 cgcaaatcca ctgatgcata tcggtgaatc gaaccgtttt cggatcaaga tcgggatacg
     2941 catcgtacag tgcttcgcca atttcgcggc tatcggtcca cttaagtccc atactaacct
     3001 ctgttaatgc tcacgcgcat ggttgatagt gtaacgcggg atttcgacca ctaaatcttc
     3061 gtcggtaacg cgcgcctggc agcttaaacg gctttccggc tccagtcccc aggctttgtc
     3121 cagcatgtcg tcttcctgct ctgagctttc cggcagcgag tcaaaacctt cgcgaacgat
     3181 gcagtggcag gtggtgcaag cacaggattt ttcacaggcg tgttcaatct cgataccgtt
     3241 acgcagagct gcgtcgagaa tggtttcacc gctattagct tccagaacag cgccatcagg
     3301 gcagagctcc tgatgaggca aaataacaat ctttggcata ttaaacctcg tccacggaat
     3361 ggcctttcag cgcacgacga accgactggt ccatgcggcg agcggcgaaa tcctgggttt
     3421 gtttgtctac gtttttaatc gcttgttcga tggcgtcaac atcatcgccc tgcgccactt
     3481 cactcaggtg agcggcagca tcgtcaatga cctgacgttc tgcggcgctt aacagcgcgg
     3541 catcagcagc cagcgcgccg tgcagacttt ccagcacacg cgccgcttct actttttgtt
     3601 ctgccagcat tcgggctttt acgtcctgct cggcatagct cattgagtct ttgatcatcg
     3661 aagcgatttc gctatcggtc agaccgtaag acggtttgac ctgaatagac gcctcaacgc
     3721 cggtggattt ctccatcgcc gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca
     3781 cgcgaatatg cgcaccgcca gccggtagcg ccggaatacc acgcagcgca aaacgcgcca
     3841 gtgagcggca gtcctgcacc agttcgcgct caccctgcat tacatggata gacatcgccg
     3901 tctgaccatc tttaaaggtg gtgaaatcct gagcgcgggc caccggaata gtggtattac
     3961 gcggaatcac tttctccacc aggccgccca tcgtttcgag gcccagcgac agtgggatca
     4021 catcaagcaa cagcatttcg ctgtctggct tgttacccac cagaatatcc gcctgaatcg
     4081 ccgcgccaat agcgacgact ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa
     4141 attcgcctac ccgttcacgc accagcggca cgcgagtaga accgcccacc atcaccactt
     4201 ccagcacttc atcagcttct acacccgcgt ctttcagcgc gcgacgacaa gccagtaagg
     4261 ttcgttttac cagtggcgcg atcagttcat tgaattgttc acggctgatt tcgccctgcc
     4321 agcccgcaac gttaacggtc acggagtccg catcgctcag cgcgattttg gctgcaatgg
     4381 cggcatccag cagttcacgc tgaacgcggt tatcgctacg atcaggaatg cccgcctgct
     4441 cgcgaatgta atccgccagc agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc
     4501 cggttgccag cacttcaaac acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc
     4561 cgccaccgag gtcataaacg gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga
     4621 tagccgcagc ggtcggttcg ttaagtaagc gcaggacgtg aaggcccgcc agacgcgccg
     4681 cgtctttggt gccctgacgc tgggcatcgt caaagtacgc cggaacggtg ataacgacac
     4741 catccagctc gcctgccagg gcttcagttg cccgcgccgc cagtgctttg aggatgtccg
     4801 cagaaacgcg caccgggttc agcagccccg ccgccgtttc aatcatcggc aggccgtttt
     4861 cgctggcctg gaattgataa ggcagatgcg gatagcgttg ctggatatca gccagcgagc
     4921 gtcccatcag gcgtttaaca gaactaattg tgttggcggt atcgagcgct gcattagtac
     4981 gcgcgtcata acccaccgaa tgcccttgct gttgatagtg aacaacagat ggcagcaggt
     5041 gacggccttc atggtcggct aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg
     5101 agttggttgt gcccaggtca ataccggccg ccagacgacg ctgatgcggc gcagcactca
     5161 aaccaggttc actaatttgt aataaggcca tgtttagctt ccagaaatta aaaatcgagc
     5221 agtttttctt cgagttgttc ggcactgctt cgcagtttat cgagaaaacg cagcttacgc
     5281 acggtatccg ccgccgcgtc ccaggtctcg ttgtctaact gttcaaccat caactgatgg
     5341 cgggtatcaa acatcttttt cacacgtttg ataaagcttt ccagccgcgc ttcatctttc
     5401 gcctgttcga tctcgtccag ctcttcgcgc agctccaact gttccatcag gaacgcggtg
     5461 tcgcgcacag tatgctgctc gctggcgaga tcaaagccgt gcaaagaaag caaatattcc
     5521 gcgcgcatta acggatgacg cagcgtttgc caggcctggt taatggttgc agattgctgt
     5581 acggcggcga gttgttccgc ctggcttccg ctggcgaatt tatcaggatg atactgacgt
     5641 tgtagatcct gaaaacgcag gctcagcgcc tgggtatcga gttgataacg ggcaggcaag
     5701 ccaaagaggg tgaagtaatc cataacattc tcagggctgc gatccgccgc gataacggcg
     5761 aatcaggtaa aacaaacccc acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc
     5821 aaacgtggaa gctttcgccg caaccacact catctttaac gttcgggttg gtgaatttaa
     5881 acccttcgtt caggccttct tttacgaagt ccagctgcgt accgtccaga aattgcaggc
     5941 ttttgccatc gaccacgact ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg
     6001 gttcgtcaac aaattccagt acataagcca tacctgaaca tccggaggtt ctcacgccca
     6061 gacgcaggcc aaaccctttg ccgcggttag ccagaaaggt atttactcgc gctgctgcac
     6121 tgtcgctcag tgtaatcgac ataaccaaac ctcaactctt attttgcttc acgtttgctt
     6181 ttatagtccg caatggcggc tttgatcgcg tcttctgcca gaatagaaca gtgaattttc
     6241 accggcggca gttcaagttc ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg
     6301 agagacttcc ctttcaccca ttcggtgacc agggagctgg aagcgatagc ggaaccgcag
     6361 ccgtaagttt taaaacgcgc gtcttcaatg ataccttcat cgttgacttt aatctgcaac
     6421 ttcatcacgt cgccacaggc cggtgcaccc accatgccgc tgccgacgtt ctcgtcgttg
     6481 ttgtcaaagg aacccacgtt acgcggattc tcgtaatggt cgataacttt ttcgctgtaa
     6541 gccattataa attctcctga ttccgatacc gattaatgat gagcccattc gatgctgttc
     6601 agatccacgc cctgcttgta catttcccac agcggagaaa ggtcacgcag acgaccgatg
     6661 gatttacgaa ctaactcgat ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa
     6721 gagaaacgga tagagctatg tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag
     6781 gacggttcga ggcttgctga cgtacaggcg gagccggaag aaaccgcgag gtctttcagc
     6841 gccataatca gcgactcacc ttcaacgtag ttgaagctga cgttgagaat gttcggcgca
     6901 ccgtgttcca ggtcaccgtt caggtaaact tcttcgatat ctttgatgcc gttccacaga
     6961 cggttacgca gtccgcgcag acgttccatc tcggtcgcca tctcttcttt tgcgatgcga
     7021 taggcttcgc ccattccgac gatctggtga acaggcagag tgccggaacg cataccgcgt
     7081 tcgtgaccgc cgccgtgcat ttgcgcttcg atgcgtacgc gcggtttacg acgcacatac
     7141 agcgcaccaa tacctttcgg gccatagatt ttgtgaccgg agaaagacat caggtcaact
     7201 ttcaactggc tcagatcgat aggcagtttg cccacgctct gggttgcatc aacgtgatag
     7261 ataatgccac gagcacggca catttcgccg atagccgcga tatcctgcac cacgccgatt
     7321 tcgttattca cgtgcatgat agaaacgagg atggtgtcgt cacgcatcgc cgcttcgagt
     7381 tctttcaggt cgataatacc gttacgctgc ggtgccaggt aggtgacttc gaaaccttcg
     7441 cgctccagct gacggcaggt atccagtacc gctttgtgtt cggttttgct ggtgatgatg
     7501 tgcttgcctt ttttctgata aaagttggct gcacctttga tcgccaggtt gtcagattcg
     7561 gttgcaccag aggtaaagac gatttcacgc ggatcagcgc cgaccagatc ggcaatctga
     7621 ttacgggcga tatctaccgc ttcttcagcc tgccagccga aacggtgaga acgggaggcc
     7681 gggttaccaa aggttccgtc catcgtcata aactgcatca ttttctcggc aacacgcggg
     7741 tccaccggcg tggttgcgga gtagtcgaga taaatcggta atttcattgc tctataaact
     7801 ccgtacatca ctcaatgcaa ggaatcaggc taccggctgg atgtacgacc gtgtttacgg
     7861 agtatttagc actccggcct gattctgaat tctttttatt aagcgcgtaa cttaacgtcg
     7921 atcgcgtctt gtgtgcgggt gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc
     7981 agcacttcct ggttattaac cagttcgcct aaagtaatgt tgttgagaaa accggtgaga
     8041 cggtcgctca aatcacgcca cagcgcgtgg gtcaggcatt tatccccgcc ctggcagccg
     8101 cctttaccct gacaacgggt agcatctaca gattcgtcaa cggcgctaat cacttcgcca
     8161 acggcgatgc tgctggcatc tttgcctaac agataaccac cgcctggtcc acgtacgctg
     8221 gaaaccagac catttttacg cagacgggaa aacagttgtt ccagataaga aagggaaatt
     8281 ccctgacgtt cggaaatatc agccaacggt accgggcccg cttcagagtt gagcgcaacg
     8341 tcaagcattg cggtcacggc atagcgccct ttagatgtca gtctcatgtc ttacttcacc
     8401 tcaaactcgc ccctgcccgg ggttttttat tgtaaaagtg ggggtattgc atagcagggt
     8461 caagtctgac attcccgagt aaattggtca actatttact tgactgattt agtcgggtat
     8521 ttaaccttca gtgccatttt tttatcgtgg cgtttgtacc tgttgtcgga tgcggtgtga
     8581 acaccttgtc cgacctggga ttccgtgcac ttacaggcct gataagacgc gttaagcatc
     8641 gcatcaggca accactgtta ttcggcctta ttacctttat tctgctgctc tatagaagcc
     8701 agaatcccgc gcaggatatt caattcctgg ctttccgggc gcgcacgggt aaacagacgg
     8761 cgcaatttat tcatcacctg ccccggatgg ttttcacgga taaaaccggt tgccagcagc
     8821 gtttgttcca gatgaccata aaaacgctcc agatcatcga ccagcggata cggcgtctct
     8881 tcatgctcga cctgctcgcc attttcctga gttgccagcc aggccatacg cacttcatag
     8941 gcaataacct gaaccgccat tgccaggttc agcgaactgt attccgggtt tgccgcaatc
     9001 gcgacatgat aatggcattt ctgcaactct tcattggtca agccgacgcg ctcacgacca
     9061 aacaccagcg ccaccggggt atttgccgct tcagcgacac ttttcagacc gcattcgcgc
     9121 gggtcgagca tcggccacgg cagcgtgcgg gagcgtgcgc tggtgcctac caccaggcta
     9181 caaccggcta acgcttcgtc gagcgtatcg acgatgtgag cattaccaat cacatcgctg
     9241 gcccccgccg ccagggcaat agcctgggag tcaggtttca ccagtggatt aaccagccac
     9301 agattggtta atcccattgt tttcatggca cgggcaacag aacccatatt gccggtgtgt
     9361 gacgtctcca ccagcacaat tcgaatattt tgcagcattg tctttcttcg tctaaagatt
     9421 attcgcgcat cttatcataa aacgaagaca gatgccgatc tcgctgctat actctgcgcc
     9481 gttttcccgt tctttaacat ccagtgagag agaccgatgc atccgatgct gaacatcgcc
     9541 gtgcgcgcag cgcgcaaggc gggtaattta attgccaaaa actatgaaac cccggacgct
     9601 gtagaagcga gccagaaagg cagtaacgat ttcgtgacca acgtagataa agctgccgaa
     9661 gcggtgatta tcgacacgat tcgtaaatct tacccacagc acaccatcat caccgaagaa
     9721 agcggtgaac ttgaaggtac tgatcaggat gttcaatggg ttatcgatcc actggatggc
     9781 actaccaact ttatcaaacg tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa
     9841 ggccgcaccg aagttgctgt ggtatacgat cctatgcgta acgaactgtt caccgccact
     9901 cgcggtcagg gcgcacagct gaacggctac cgtctgcgcg gcagcaccgc tcgcgatctc
     9961 gacggtacta ttctggcgac cggcttcccg ttcaaagcaa aacagtacgc cactacctac
    10021 atcaacatcg tcggcaaact gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg
    10081 gcgctggatc tggcttacgt cgctgcgggt cgtgttgacg gtttctttga aatcggtctg
    10141 cgcccgtggg acttcgctgc aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc
    10201 gacttcaccg gtggtcataa ctatatgctg accggtaaca tcgttgctgg taacccgcgt
    10261 gttgttaaag ccatgctggc gaacatgcgt gacgagttaa gcgacgctct gaagcgttaa
    10321 tgactcaggc gggtgatatc actcacccgc cctcgccttt caggcgctat tccgaaatac
    10381 ttcctcaccg ctttactttc tttcgtcact ctcccaccat ctttccccga ttaatggatg
    10441 ataaagaagt cgcaaggaaa ggttatggca ctgccagtga acaaacgcgt tctcaaaatt
    10501 ctgtttattc tctttgttgt tgccttctgc gtttatttag tgccgcgcgt tgccatcaac
    10561 ttcttctatt atcctgacga taaaatttac ggtcccgacc cctggtcggc ggaatccgtc
    10621 gaatttacgg ctaagaacgg tactcgtctg caaggctggt ttatcccttc ttcgacgggc
    10681 cctgctgaca acgccatcgc aaccatcatt catgctcacg gcaatgccgg aaatatgtcc
    10741 gcccactggc cgctggtcag ttggttaccc gagcgtaatt tcaacgtttt tatgtttgat
    10801 tatcgcgggt ttggtaaatc aaaaggcacg ccgtcccagg ccggattgct ggacgatacg
    10861 caaagcgcca tcaatgtggt gcgccatcgc agtgatgtaa acccacaacg gctggtgctg
    10921 ttcgggcaga gtattggcgg ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa
    10981 ggcatacgtg cggtgatcct cgactccaca tttgcctctt atgcaaccat cgccaaccag
    11041 atgatccccg gcagtggcta cttacttgat gagagttaca gcggcgaaaa ttacatcgcc
    11101 agcgtcagcc cgatcccgct tttactcatt cacggtaaag ctgatcacgt tatcccatgg
    11161 cagcacagcg aaaagttgta tagcctggca aaagagccaa aacggttgat cctaatcccg
    11221 gatggcgaac acattgatgc tttttccgat cgtcacggcg atgtttatcg cgaacagatg
    11281 gtggacttta tccttagcgc gttgaatccg cagaactaac ccatgatcgc tagcacgata
    11341 atcattcaca aaaccacctt aagacatgct aatccactgg tcagaacagt ttaagatgag
    11401 aaaaattctg tgacgcttgc caacatttct gatgattagc attcccttcg ccatttccct
    11461 gagcaaactt tagctattct tatcaattat gcttatggga gatctacaga tgatgcctac
    11521 gcttgctcca ccatctgtcc tttcggctcc ccagcgccgc tgccagatct tgctgacgct
    11581 ctttcagccg gggttgaccg ccaccatggc aaccttcagc gagcttaatg gtgtggatga
    11641 tgatattgcc agtcttgata tcagcgaaac aggacgggag atcctgcgct atcatcaact
    11701 cacactgacg actggttatg acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg
    11761 tttgtgttta tttcactggc tacgacgtgg tttccgtctg tgtccgtcat ttattaccag
    11821 ccaattcacc cccgccctga agagtgaact gaagcggcgc ggaattgcgc gtaactttta
    11881 cgacgatacc aatctacaag cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt
    11941 tgaatcgcgc gatattcatt tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca
    12001 cgctggaatt acgcctcagt ttaatccgct ccaacgtcgc tgggcggaat cctgccttga
    12061 atttcaggta gcgcaggaaa ttggacgcca ctggcagcgt cgggcgctcc agcctgtacc
    12121 acctgatgag ccactgttta tggcactact tttttccatg ttgcgggttc ccgatccatt
    12181 gcgggatgcg catcagcggg acagacaatt gcgtcagtct atcaaacgtc tggtaaacca
    12241 ttttcgtgag ctgggaaatg tttgttttta tgatgaacag gggttatgtg atcagcttta
    12301 tacccacctc gcccaggcgt taaatcgcag tttgtttgcc atcggtattg ataataccct
    12361 gccggaagag ttcgccagac tgtatccacg cctggtacgc accacccgcg cggcgctggc
    12421 cggatttgaa agtgaatacg gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt
    12481 gattttcggt gcctggctaa tgcaggaaaa cgatctgcat gaaaaacaga ttattctact
    12541 gaccgggaat gatagcgagc gagaagcgca gattgagcag cagttacgcg aactaacgtt
    12601 actgccgctc aacattaaac atatgtcggt aaaggcattt ttgcagacag gcgctccgcg
    12661 tggcgcggca ctgattattg ctccttatac catgccgtta ccgctctttt caccaccgct
    12721 gatctatacg gacctgacac tgacaacaca tcaacaggag cagatccgca aaatgctcga
    12781 atcagcatga gggaacaact ttcgggcgca aaaacattgc cggaagcgcc accagcgcca
    12841 tcacccagaa cacgccgtgg cccagatatt gatacaggaa accggcgaaa acggtcatga
    12901 tagcgatact gccgcccatc gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc
    12961 taccctggcg agcggcaata tagcgcatgg cggccaggtg gcagaccgtg aaggtgccgc
    13021 aatgcagaat ttgcaccact atcaaccacg gcaacgccgt agttgctccc ataatgcccc
    13081 agcgcactac gccgcaaatc gccgagatca acagcatatc gcgtgcacta caacggcgga
    13141 aaagtttatt actcagtgca aagataatga cttccgccac cacgcccagc gaccacaaat
    13201 accccaccgc cgaggccgag tagccagctg cctgccagta aatggcgcta aaaccgtaat
    13261 aggccgcatg cgccccctgc aataaacaaa cgcaggccag aaagcgccag ttctggcgaa
    13321 ccagcgccaa ccacgctgac caaccggtgc tctcctgttg gcggcttgcc ccttgtggct
    13381 gaatcgtcgg acggatgaga aagccgagca gcatggatgc cacgcccaac gtcaacagca
    13441 cgaggatcac ccgataatca aacatagtga ccagtttgcc cgtcagcgcc gagccaatga
    13501 caaacgccac cgagccccac agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc
    13561 acgtattcgc cagtgcatcg gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa
    13621 tcatcaccag catcagccac gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa
    13681 gtgtcagcag tgccagcacg cgcaaggcgg aaatcaggcg agaaggatcg ctgacgcggg
    13741 gcgcgatgag caaactcccg agaaaacggg caaccagacc tgcccccaat aacaggccga
    13801 tggtttctgg cgttaaacca atccctttaa gccagacgct ccagaaaggt agaaaaatgc
    13861 cgtaactaaa aaagtatgtg aaatagccga gcgccaacca gcgcgtggat tgcaaaacca
    13921 tgagtccctc ccgtcaggag gcgttagtct ggagttaatc gaatgttggt gcaagttgaa
    13981 aaataagaat attcgagcct gcttcccgat gtctgtaacg ccagttcaca agcactcaaa
    14041 aactgcgatc cgtgacgcat gttgcaaaga taatgtgtaa ttatgccgtt acgcttgcca
    14101 aacgttcctg cacaatggcg atgaaatcgc gtaatgccgg cttcatctcc cctttcttcc
    14161 acgccatcag taaagcaatg gaaggcacat ttccggcaat gggacgaaaa acaacctgcc
    14221 cggtattaaa attattcata taaccgggta tcaaagtgac gcccagcccc atgcccacca
    14281 gattcatggt caccagaata ttcgttgcca cctggacgat atttggctgg ctgttttctt
    14341 gtgcaaacca cgctttaacg atcggcgcaa gcgaaccgga atacgccgga tcggtactga
    14401 cgaaattcac gccatccagt tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat
    14461 caaccggtaa caccaccact aatggttcgt caaaaagctc cagataatca atctccgggc
    14521 tataaacagg atggcgcatt aagccgacgt cgagttcacc acggcgaatt ttttcctcct
    14581 gttgcgtggt gattaaactc accagctcaa tcaaggtgtc tggctgtctg agacgaaaca
    14641 tcggtaatac ttttggcagt aaattcactt ccgccgatgg cacaaagcca atggttaatt
    14701 gtctgtcttc ctgaacaatt ttccgcgccc gtaatttggc attttccgct tgttcgagga
    14761 ttgccagcgc gtcctggaga aaacattctc ccgccgccgt caacgcgact ttgcgcttat
    14821 ccctcaccag taacggaaca ccgacacagt tttcaagatc gcggatctgg ctgcttaacg
    14881 aaggctgtga ggtatgcagt ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg
    14941 cgacgaaata gcgtaaatgc cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc
    15001 tcagctggta gaaattaact atttcacata ttagcaacca accagcaaca tccttatggc
    15061 acaaaaatag aaggtcaata catcttatct ttcaggatta aaaaatatga ccacaccctc
    15121 agatttgaac atttaccaac tgattgacac ccaaaacggt cgggtcactc cgcgtattta
    15181 taccgacccg gacatttacc aactggagct tgagcgtatt ttcggtcgtt gctggctatt
    15241 tctcgcccac gaaagccaga tcccaaaacc cggtgatttc tttaacacct acatgggaga
    15301 agatgcagtt gtcgtggtgc gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg
    15361 ccgccaccgg gccatgcgtg tgagttatgc agattgcggc aacactcgcg cctttacctg
    15421 cccgtatcac ggctggtctt atggcattaa cggcgagttg atcgatgtac cgctggaacc
    15481 tcgcgcctac ccacaagggt tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt
    15541 ggagagttat aaagggctga tttttggcaa ctgggatacc agcgcaccgg gcctgcgtga
    15601 ttacctgggt gacattgcct ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac
    15661 cgaaattgtc ggcggcgtac aaaagtgggt gatcaactgc aactggaaat tcccggcaga
    15721 gcagttcgcc agtgaccagt atcatgctct gttcagccat gcttctgccg ttcaggtatt
    15781 aggggcgaaa gatgatggca gcgataagcg cctcggtgat ggacaaaccg cccgcccggt
    15841 gtgggaaacc gccaaagatg cgctgcaatt tggtcaggac ggtcacggta gcggtttctt
    15901 ctttactgaa aaaccggatg ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg
    15961 cgaaacctat gccgaagcag aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg
    16021 tcataacaat attttcccca cgctttcatg gctcaacggc actgccacgc tccgcgtctg
    16081 gcatccgcgc ggccctgatc aagtcgaagt gtgggcgttc tgtattactg acaaagccgc
    16141 ctccgatgaa gttaaagccg cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg
    16201 ttttctcgag caggatgact cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca
    16261 ccgcgcccgc aacagcaaac tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga
    16321 cgacggcatt cctggcatta ctaactatat cttttcagaa actgccgctc gtggaatgta
    16381 ccaacgctgg gccgatcttc tgagtagcga aagctggcag gaagtgctcg ataaaaccgc
    16441 cgcttaccag caggaggtga tgaaatgagt gcgcaagttt cactagagtt acatcaccgc
    16501 attagccagt ttctctttca cgaagccagc ttactggacg actggaaatt tcgtgactgg
    16561 ctggcgcagc tcgacgaaga gattcgttac accatgcgca ccacggttaa cgcgcaaacg
    16621 cgcgaccgcc gcaaaggcgt ccagccgccg acgacctgga tttttaatga cactaaagac
    16681 cagctggagc ggcgaatcgc ccgtctggaa acgggcatgg cctgggcaga agagccaccg
    16741 tcacgcaccc gtcacttaat cagcaactgc cagataagcg aaaccgacat cccaaacgta
    16801 tttgctgttc gggtgaatta tctgctttat cgggcacaaa aagagcgcga tgaaacgttc
    16861 tatgtcggga ctcgttttga caaagttcgc cgtctggaag atgacaactg gcgcttgctg
    16921 gaacgggata tcgtcctgga tcaagcggta atcacttccc ataacctgag tgtactgttc
    16981 tgatgaatcg aatttatgcg tgtcccgttg cggatgtgcc ggagggtgag gctctccgga
    17041 tcgatacctc gcccgtcatc gccctgttca acgttggcgg cgagttttat gccattaacg
    17101 atcgttgcag ccatggtaat gcgtcgatgt cagaagggta tctggaagat gacgccacgg
    17161 tggagtgccc gctacacgcc gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc
    17221 ccgccaccga tccgctcacc acttatccag tacacgttga aggtggtgac attttcatcg
    17281 acttaccgga ggcgcagcca tgagcgatct gcataacgag tccattttta ttaccggcgg
    17341 cggatcggga ttagggctgg cgctggtcga gcgatttatc gaagaaggcg cgcaggttgc
    17401 cacgctggaa ctgtcggcgg cgaaagtcgc cagtctgcgt cagcgatttg gtgaacatat
    17461 tctggcggtg gaaggcaacg tgacctgtta tgccgattat caacgcgcgg tcgatcagat
    17521 cctgactcgt tccggcaagc tggattgttt tatcggcaat gcgggcatct gggatcacaa
    17581 tgcctcactg gttaatactc ccgcagagac gctcgaaacc ggcttccacg agctgtttaa
    17641 cgtcaacgta ctcggttacc tgctgggtgc aaaagcctgc gctccggcgt taatcgccgg
    17701 tgaaggcagc atgattttca cactatcaaa tgccgcctgg tatcccggcg gcggtggccc
    17761 gctgtacacc gccagtaaac atgccgcaat cgggcttatt cgccaactgg cttatgaact
    17821 ggctccgaaa gtacgggtga atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg
    17881 cccacaggcg ctcgggcaaa gcgaaacctc gataatgcag tctctgacgc cggagaaaat
    17941 tgccgccatt ttaccgctgc aatttttccc gcaaccggcg gattttacgg gtccgtatgt
    18001 gatgttggca tcgcggcgca ataatcgcgc attaagcggt gtgatgatca acgctgatgc
    18061 gggtttagcg attcgcggca ttcgccacgt agctgctggg ctggatcttt aaggaagtac
    18121 gatgaaagaa aaaacgatca ttattgtcgg tggtgggcaa gcggcggcaa tggctgcggc
    18181 ctcgctacgc cagcaagggt tcaccggtga gctgcatctg ttttccgatg agcaacatct
    18241 tccttatgaa cgccctccgc tctcgaaatc catgttgctg gaagattccc cacagttgca
    18301 gtctgtgtta cccgctcact ggtggcagga aaacaatgtt catctgcatt ccggtgtaac
    18361 catcaaaaca ttgggccgcg acacacgaga gttagtgtta gccaacggcg aaagctggca
    18421 ctgggatcag ctttttatag caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc
    18481 actgggagaa cgctgcttta ccttgcgcca tgccggtgat gccgccagac tgcgagaagt
    18541 tctgcagcca gaacggtcag tcgtaattgt cggtgccgga actattggtc tggaactggc
    18601 tgccagcgcc acgcagcgcg gatgtaaggt gacagtgatt gaactggcgg caaccgtcat
    18661 gggccgtaat gcaccaccgc ccgtgcaacg ctatctttta cagcgccatc aacaggctgg
    18721 tgtgcgcatt ctgctcaata atgccattga acatgtggtc gatggtgaaa aagtagaact
    18781 gacgctgcaa agtggcgaga cgcttcaggc cgatgtggtg atttacggta ttggtatcag
    18841 cgccaacgag caactggctc gcgaggccaa ccttgatact accaatggca ttgtcattga
    18901 tgaggcttgc cgcacctgcg atcccgcgat ctttgccggt ggcgatgtgg caatcactcg
    18961 tcttgataat ggtgcactac accgctgcga aagctgggaa aacgccaata accaggcgca
    19021 aattgccgct gccgcaatgt tagggctacc gcttccgcta ctgccgccgc cgtggttctg
    19081 gagtgatcag tacagtgata acttacagtt tattggcgat atgcgtggcg atgactggct
    19141 ttgtcgtggc aacccggaaa cccagaaagc gatttggttt aatctgcaaa acggcgtgct
    19201 tatcggtgcg gtaacgctga atcagggacg tgagattcgc tcaatccgca aatggatcca
    19261 gagcggcaaa acgtttgatg cgaaacagct gacagatgag aacatcgcgc ttaaatcact
    19321 gtaaccagga taattagcga atatctcaac gcctggggcg tggcgaggta ccagagtgtg
    19381 tattacgttt aaatcacatt atcttgcaaa gggattggtt atgaactcac tacgttattt
    19441 tgattttggt gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg tggtcctaat
    19501 tttcattatt tttggttttc ccaaaatgat gggctttgac ggtacggtcc aatatatggc
    19561 ctcgctgggc gcaccgatgc cgatgctggc agcgattatt gcggtagtta tggaagtgcc
    19621 cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg ctggcggtac tgtttatttt
    19681 ctacacgctg ggtacggcgg tgattggtca ccattactgg gatatgaccg gcgatgccgt
    19741 cgggccaaat atgattaatt tctggaagaa tgtcagtatc gctggcgcgt tcttgctatt
    19801 ggcaattacc gggccggggg caatttctct cgatcgccgt taggaaagat gccggatgcg
    19861 gcgtgaacgc cttatccggc attttataaa ttacagccac tctacccgca acgacatctc
    19921 tgaggttgtt gattcccccg gcgcaagcgc aatgaggtca cccccttccg ggcgatgatg
    19981 gtcatccggc gcatggctca tcggctccag acagaagaaa tcaaacgcat atcctttatc
    20041 aaacgccggg tcggaaacaa agatgaaata acacggtgca ggtggcgtcg tttctatgat
    20101 gatggcataa ccctcctgcg gctgctcgat gcgggcctga ccattccatc cggcaaaacc
    20161 attgttcacc cactggcgcg gcaacggcgc tagctggtta aaatccagtt cttgcggtag
    20221 ctgctcgcaa aactcacccg ccagccactg ctcccgctcc agccagtaac cgctcgcctg
    20281 tgcctgaatc cgcgtttgcg gcgacaacgg aaaataagga tgccagccgg taccaaatgg
    20341 cagcgtctct gccccttgat tggtgacaga gagcgtcacc gtcagcgtat ccgccgttaa
    20401 atgaaacgcc tgacttaccc gatagtgata gacaccgctg cgatgttcat acaccagaca
    20461 caaactatca tcgctgtgcg agacacattg ccactcgccc agccagccat cgccgtgtag
    20521 atagtgcgca tcccactcaa cattcggttg cagttgatac tcacgcccct gccagacaaa
    20581 atggttgccg ctcacccggt ttgcaaatgg cactagcgga aaacaagagg catcggttgc
    20641 cacaccgctt tttttaccag gacgtaagag cggcgtcgta tcacgccaga aaccttcgat
    20701 gacaccgccc tggtcagaga catccagctt tagcggtccg tgagataagg tatagatggt
    20761 catccgctaa ctccttaatc cgggaagtta atcacaactt tcccgcattt gccgctcgcc
    20821 atcagcgcat aggcatcacc tgcctgttcc agcgagaagc gatgggtaat ggcgttacgc
    20881 ggccacagct tccagtcagt caaatcgtgg gcgcattttt ccatatggaa cagactggtc
    20941 acccaggagc caataatccg ccgttgatgg tgcatcagat cggcgctgac ctcgaattcc
    21001 acttttccgg tttcaccaat gtaaaccacc cgcccccagt cagcagtgga ttgcagtgcc
    21061 agcaagcgac ctgcggcatt accggaacaa tcgagcgcaa catccgcgcc accgtgggta
    21121 agttcagcaa ttatctgcgg cagaccttcg gtggtcgcta aataaccgtg ctccatcacc
    21181 cctaactgtt ttgccatcgc cagacgttcc ggcagcatat caacgccgat gatccgtttt
    21241 gcaccgcgac ctttcgccag catcatcgcc atcatgccga ccggccccag accgaccacc
    21301 agcacgttat cgctgccgga aacttcgccg cgcaaaatcc cttcataggc cgtgccaacg
    21361 ccgcaactga taaacgcacc atcttcgtag ctcagcgcat ccggcaggag gatcagatct
    21421 ttttcttccg ccagcagata ttcggcatga ccgccgtcac gctgccagcc gtaagccgct
    21481 tttcctttgc cagtacaaga gataggaaag ccgcggcggc agttcgggca aaaaccacag
    21541 ccagaaatgt gatacaccag cacgcgatca ccctctttaa aatggcgaca gccttgcccc
    21601 atcgccacaa tttgcccgca cggctcatga ccgttgataa agccctggta taacggttta
    21661 tcgggcgccg ccgccgtggc gcggtgttga tggtagatat agtggacatc gcttccgcaa
    21721 atcccggagg atttcatttt gatcagtacc tggttaatcc ccggcgtcgg caccgcaact
    21781 tcccgcagat cgacggtcga atttcctggt aaataagctg ccagcatcgt tttcataaat
    21841 ccctcattaa caatacgatt aattttcatc cctgccgcac ccgcgccagg ggcgttaagg
    21901 tttagcgttt cgctttactg ctacgctggg tcagcaagat attcgccagc accgccacca
    21961 cgatgatgac gccgcgcacc acctgctgga aaaaggagtt aatcccgagc agcaccagac
    22021 cgttaccgat tagcgtaatc accagtacgc caagcagcgt accgaacaac gagccgcgac
    22081 caccggaaag cgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac tccagaccgt
    22141 ttgcggcacc tgcgttaccg gaaccgaggc gcgccgccaa caaaatgccg gtcaccgccg
    22201 ctaataatcc cgaaagggta aagataagaa tgcgtacccg acgaacgtta ataccgcaca
    22261 actgcgccgc cgtggcatta ccgccaacgg caaaaaccga gcgcccgaag gcggtttttc
    22321 ggctaatgaa cacaaacagc gcaaacagca ccatcatgat cagcgcggat accggcacac
    22381 cgagaaattg tccgcccagc cagtccagca cctcgttttc gtcaatcggc actggcaggg
    22441 cgttggtcat aaacaagccc attccgcgca gggcgctcca cagccccagc gtggccacga
    22501 aacttggcac gttaaacacg ccacgcagca ccccggcgag cgttcccatc agcgcgccta
    22561 acagcaacac cagcagacaa gcaatcgcca acggaacttc aaattgcagc aaaaatgcca
    22621 ggcacaccga gacaaaagcc accatcggcc caacgctgac atcaatttca ccggagataa
    22681 taatcagcgt catcgcccag gcggcaatcc caatggtggc ggcatcgcgc agcacgttca
    22741 tctggttatt caatgagata aagccaggcg cgttcaggga gaagaccaga taaagaatgg
    22801 caatcaccac cagcaaaccg atctcattaa tatggcgact gacaaattgt ttgagcgaga
    22861 cgctcttgcc ctgcggcaat ggtaatgacg aagcagacat cttaggttcc tcggtaaaat
    22921 tcatcagtgc acagacagaa tggcggacat cagctcatcc acattgaccg gagcgtgaaa
    22981 ctcctgcgag aacgtaccgt gctgtaacaa cagaatgcgg tcgcacacca gcggtaactc
    23041 ctccacttca ctggagataa acaccacgct ttttccttcg gcagccagct cacggacaat
    23101 acggtaaatc tgctgtttgg cttcgatatc gacgccgcgc gtcggctcgt cgagcaacaa
    23161 aatccggctg gcggcataga cccaacgacc gatcaccact ttttgctgat tgccgccaga
    23221 aagcgtaccg atgggcgttt cactactggc ggccttgacc gtcatccgct gcatcacctc
    23281 ttcggttagg cggcggatgg tggaccattg cagcacaccg ttggcgctga ttttttgccg
    23341 attggtcagc actgtatttt cgtcaacgcc caaccaggga atgatccccg cttctttgcg
    23401 gttttctggc gtatagccaa tgccgcgttt cagcatgtcg ccgtaatcgg ggtgcgtgat
    23461 tttctcgccg ttgataacaa tttcgccctg ttcatactcc tccagcccaa caatcgcttt
    23521 cagcaattca ctgcgtcctg cccccagcag gccagcaatg ccgagcactt cgccacgacg
    23581 tagcgtaaag ctgatgtcgt ccagcttggg cttatggcgt aacgcacgga tttccagcac
    23641 ggcctgatcc acaatttcct gaggggcaac cggcgcaata tcaacgtgat cgcgcccgag
    23701 catcagcgac acaatatgat gcgtggaggt gttttcgagc atcacatcgc ccgccacctg
    23761 accatcgcgc atgacggtgg cacaggaggc aatgcggcga atttcttcca tccggtggct
    23821 gacataaatc accgccacgc ccagtgccga catctttttc acggcgctta tcaccagttc
    23881 aacttccgca ctggcaagcg aactggtagg ttcatcaaga atgaccacgc gcggttcgcc
    23941 tttcatcacc cgcgcaattt ccaccagctg cttttgcgcc gggcttagcc ctgaaacaag
    24001 ttgttcaggg ctaacgtcaa cgcccagcgc ctgtaagcaa cgttgggcat cctgcgccat
    24061 ttgcaggtaa tcaatcatgc cgttgcggcg gggccactga ccgaggcaga ggttttccgc
    24121 cactgtcagc ccttccacca aacttaactc ctgataaacc gcgcgtaccc ccagttcagc
    24181 ggcacggcga gtcaacgtag cttcgtcgcc ttccagtcgc gtctcaccaa tccagatatc
    24241 accgctatcc ggacgttcgc taccggtaag cattcgaatg agggtcgatt tgcccgcgcc
    24301 gtttttgcct aacagcgcac gaacctcgcc tttattgagc gtgaagttaa cgttatccaa
    24361 cgcaacgacg ccgggataac gcttatttcc tgccaccatt tttgctaccg ggactgcctc
    24421 tgttgccgtg aacataggga cctctgcgaa tcagcgatta gggcagacca tcaacgtgcg
    24481 ttgccagcca ctgtttaccg tcttccgttt tggtatagag atcgattggc acctgaatca
    24541 ctttttcacc gtcggcttgt ttattgataa ccttcaatgt ttgcgcgaaa acagcattgc
    24601 ccattttctt accggaaata tccactaccg ctttcagcac ctgattgttt tccagctcct
    24661 gagtaatttc ggttgtcata tccgaaccga aaacagcaat ttttccggcc tgattttgat
    24721 tacgtaccgc ttttaccgcg ccgagtgtcg caccgcccga ttcccccata atggcgttga
    24781 gatccggcgt ggagataatc agtttttcac caacggaaat cgctttatct aaaacagtcc
    24841 cttcctgatt agcgacaaat tgcgcgccgg gaacgcgggc ttttaatact tcttcaaacc
    24901 ctttacgtcg ttgcacacaa acttcaaagg cttcgcaatt gatgacggca attttcggct
    24961 ggtcaatttt gttggcaata aaataatcgg cggcagcgtt acccagtttt ttaccaaatt
    25021 ccagcgggtc acccaccaga tatgccgaga catatttatc gacccccttt tgattaatac
    25081 aggtgttgta gcaaatcacc ggaatacccg cttcactggc gcgacgaacg gtacggctac
    25141 tgccattttc agacactgcc gataaaataa tggcatcgac attacgcgcc acgagggtat
    25201 caacaaaggt actttctttc gaaatatcac cctgggcgtt agtttcaatt aactgtacct
    25261 gtactgaaga atcttttgcc gcatcctgaa cgccctgacg cactccagcg taatatccct
    25321 gagtatcaag gtatattgcg ccaatggtca tttctttttc cgcagccctg gcaaataaag
    25381 cgctacctaa tagcgtagcc attaataata gattacgggt tgttctcatt tttttaggca
    25441 tagagcctcc tgtagggttt ttattaacaa cggcttattc taattatttt gtgatgagcg
    25501 gcagcgcgtg ccgctcaaat attacagtta atagggttta gtgaacgtag ttaaaaataa
    25561 aaggcatcac ggtggtgaat aatgccgctt tcggccaggc cggattaatc gccgtcaatt
    25621 cagcgcgagc agactcatat tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca
    25681 ccatcaggca tttttcttta tgctgctgtt gtaaatcgcc atacagcgac aacagatcag
    25741 gttgtgaaac agcaaagaaa tccgcctcaa tactggtttt cgccatctct tgcgcccact
    25801 gtttcatttc actaaacagt tgctgtgcgg tgtgttgttc gcccagtaat cgcagcgcca
    25861 ttccttgcca gaagaggtaa tcaaccggct gatcgttgta atagctgtgg atgttaatgg
    25921 tgcgatcgcc ggtcgccgcc agacgtaaac agcgcgtcgc ttcagtttca tcacccaggg
    25981 ctttggcaca tatcgcctgc cagaaccaga tgtcgttgtc agtttgcccc ggtaatcggc
    26041 cttcgcttaa attctccgga tagtgcagcg cggcatgaag cagtttgctg gcctgctgcg
    26101 gttgtctggc atcaaggtgt tgccaggcgc gtaacaactg gttgaggata aactgactgg
    26161 tgactttccc ttctccgcct tcccacgggt ggaatttgcg cgtggcgaga atgtccgccg
    26221 ctttgtctgc ctgccccgtg agatgccaca aattgagcag ttctgcggtc atgtcgtcgc
    26281 gtttcagcgc aatttccaga ttattttcca gacgcgccag tcgtttctcc ggtgtggctc
    26341 cacttaactt atcaagcaaa tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct
    26401 gataagcatt atcaagataa cgcgcggcca gctcataatc gtgttgctta ttccacgcat
    26461 ggatcgctaa cccgcgccag ccgtcggcaa actccggcga catctctacg caacgttgcc
    26521 ataaggcaat ggctttgttg tagctacgtt tgttgtagta gaagcaggcc agtaaatggc
    26581 gagcaaacca gcactcttca atactctcca gcgccgccac ttcttccagc gtattcggga
    26641 aacggacaaa ctgcgggaag acatcaatgg cttttgcgac cagttcgcca cgttcggctt
    26701 tcggcagcaa gctggcttgc aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca
    26761 gcatctctgc cgccagggtg ggcattcccc agttaatcag ttgcccggcg gtcatcaggg
    26821 cgttaacgtc gcgtccctga cacagaccgc gccactgcgc gagagctgat tcgctacgac
    26881 catcgaacca gttcagccac cacagagtgg cgttcagcgg ataatcgcgc agcagtttct
    26941 cgcgctgcaa acgcgcgttg tcctgacgac cacttaacac cagcagcaga ttatgcaggc
    27001 aaagcacttc ctgattggtt gggcaggcgc gaagactttg ttggcaaaaa tccagacccg
    27061 cgtcgaagtt accattacgc gccgccagtc gtgccagacc ataatagcct ccggctttac
    27121 tgttgccgct ccagaccgca cgccagaaat cctcttcggc ttgttgatat tgtccctgac
    27181 gttcgtaagc actggcgcga atcaaactgg cctgtccgca ctgcggattt ttgttcagcg
    27241 catgtgcgcg tttcagcgcc tgactggcat acgccaccgc ttgcgggaaa tctgcgcggt
    27301 tatattccag catcgccagc gccagattac agcggtaatc cagcgaatcc agcgccacgc
    27361 cgcgcaggta gtaatcgaac ggtgaacggc tggcgtgatg atattgctcc agatgctgac
    27421 cgataaacca ggcttcatct gtactggtaa tgtcttgtgc tgacagtggc gctttggcga
    27481 cgtccggcaa cggcaactct tgcggctgat gttcctgata actcagtaca atattgccgt
    27541 cggcatcgga gagctcaatg gtcagccttt caggattgat accgtgcaac acgccctgga
    27601 tggcggtcgc tggtgtcagg gccacggcat catcaagtaa cgcgttgcat ttgccgattt
    27661 cgcggatcgc caggcggtat ccgttcaacg gagagatggc atacagcccc cactcaatcc
    27721 cccgctcact acgctggagt tttatcaccg catcgcggga ggcattttgc accatgccca
    27781 gagaatgata aggcaggaaa tactgctcaa aacgcttctc ttcgtaagca tcaagccagg
    27841 taaaatcggg ctggttatcg gcaaaaatac cggtcatcag ttcgatatac gggccgttat
    27901 tatcagttag gctcttatcc cacgcctggc caaattcact gtgtccccag ctccactgct
    27961 ttttacctgg cgcaatatgg tggttggcaa cgtgtagcaa accgccatct tcatcgtgac
    28021 accacgcgcc aacaaaatcg tactgcgatt tttcggccat atatgaggtt ggaacgggca
    28081 catttttata gcgagaaatg tccactccag cggagtagtc cactttgtag taagtgccgg
    28141 tggcgatggg gaaagcggag acggcccgtt tgccgtgatc aaacaccgca gtcacatccg
    28201 gcgggaaaac gctctgatgc ccttcccccc ctttcactgc cgggttggcc caccacaaga
    28261 aatgacgcgg cgtggcgttg ccgttataga cgcggctggc gatttccagc gccgcccggt
    28321 cagggcgcag ggtgaaacct gtcatcacct gtaaaccatg catcggctcc gtttcgccta
    28381 cccacaccgt ctgtgcgccg tcttcatggg cttcgagggt gaaatcaacg ggcataaagg
    28441 tggtcgggcg atggtgttgc ggccagttaa actcaatccc gccggagatc cacggtccca
    28501 gcagccccac cagcgcaggt ttaatgactt cattgtgata gacaaaatcg cgttgtttca
    28561 ctttatccca tgcgcgatgc acccgaccgc ccaattccgg caggatcatc actttgatgt
    28621 agtcgttttc cagccacacc gcctgccagg atttcagggt tttctgctcg ctcagcgtat
    28681 cggtcacgcc gtagggataa accgcgccgg acgatccctg ataaacgcga ttttccagga
    28741 acatgggatg tatatcctgc ggcccggttt cataggtcgg tatctcaacg cgctcttgcc
    28801 acacttttac tggagtcatg gtgccctcaa tattaacaag acatactgaa ttaaaagatt
    28861 tgtggcagtg tattgaacaa tctggcaatg ttttcgcgga ataatcacgc aattaactaa
    28921 acaaggttta gtgaagatga gagcctgcat taacaaccaa cagattcgcc accataacaa
    28981 atgcgtgatt ctggaactgc tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg
    29041 gctggcgcaa atttcgattc cggcagtcag taatattttg caggaactgg aaagcgaaaa
    29101 acgggtggtg aatatcgacg atgaaagcca gacgcgcggg catagtagcg gtacatggct
    29161 gattgcgccg gaaggtgact ggacgctgtg tctgaacgtg acgcccacca gtattgagtg
    29221 tcaggttgct aatgcttgtt taagtccgaa aggtgaattt gagtatttac agattgatgc
    29281 accgacgccg caggcgctgc tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt
    29341 gtggccggac cgcaccatca atctggcgct ggcaatccac ggtcaggttg atccagtgac
    29401 aggagtgtcg caaaccatgc cgcaagcgcc gtggacaacg ccggttgagg tgaagtatct
    29461 gctggaagag aagctcggca ttcgggtgat ggtcgataat gactgcgtga tgctggcgct
    29521 ggcggaaaaa tggcaaaata attcgcagga acgggatttc tgcgtgatca acgttgatta
    29581 cggcattggc tcgtcgttcg tgattaacga gcaaatttat cgcggcagtt tgtatggtag
    29641 cggacagatt ggtcacacca tcgttaatcc ggatggcgtc gtctgcgact gtggacgtta
    29701 cggctgcctg gaaaccgtcg cctcgttaag tgctttaaaa aaacaggcgc gggtatggct
    29761 aaaatcacaa ccggttaata ctcagcttga tcctgaaaaa ctgacaaccg cgcagttaat
    29821 cgctgcctgg cagagtggag aaccgtggat caccagctgg gttgaccgca gtgccaatgc
    29881 cattggtttg agtctgtata acttcctcaa catcctcaat attaatcaga tttggttgta
    29941 cggtcgcagt tgtgcctttg gtgagaactg gcttaatact attattcgcc agacaggatt
    30001 taacccgttc gaccgcgacg aaggaccgag cgtgaaagcg acgcaaattg gcttcgggca
    30061 attaagccgc gcacaacagg tgctgggaat tggctatttg tatgttgagg cgcagttacg
    30121 acagatttga tggcgcgata acgtagaaag gcttcccgaa ggaagccttt ttcaacagat
    30181 taaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa ctttaccttt
    30241 gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca tccagccagc
    30301 cagttctttc gcttctgctt ctttgaagcc gcgacgcgta atcgccggag tacccacgcg
    30361 aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt tgttgacggt
    30421 gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt tatcgaccag
    30481 atcaaccagg aacagatggt tatcagtacc gccggaaacc actttgtagc cgcgttccag
    30541 gaacacttct accatcgctt tagcgttttt agcgacctgc tgctggtaag ttttgaactc
    30601 aggctccatc gcttctttca gagcaaccgc tttaccagcg attacgtgca tcaacggacc
    30661 gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt cgctaccacc
    30721 tttcgccagg atcagaccgc cgcgcggacc cgccagggtt ttgtgagtgg tggtagtaac
    30781 aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc ccgcaacgtg
    30841 cgccatatca acgaacaggt aagcaccgat gctgtcagcg atttcacgca ttttcgccca
    30901 gtcaaccacg ccggaatatg cagagaaacc accgataatc attttcggct tgtgttcttt
    30961 ggcttgtttt tccagatcgg cgtagtcgat atgaccggta gcatcgatac cgtaaggaac
    31021 gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt gaccgccatg
    31081 cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt acaccgcaaa
    31141 gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga acagttcttt
    31201 cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc cgtagtagcg
    31261 tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg cctgcattac
    31321 gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct gacgtacttt
    31381 ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca tttcacgctt
    31441 taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat aggcggtcct
    31501 gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct tgacaaagga
    31561 atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag agcttgcaac
    31621 cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca cattctttct
    31681 gaaaaacacc aaagaaccat ttacattgc
//
LOCUS       DAZKFU020000026        31481 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZKFU020000026 DAZKFU020000000
VERSION     DAZKFU020000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.026"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(319..444)
                     /locus_tag="ACHOQQ_RS04875"
                     /old_locus_tag="pgaptmp_000975"
     CDS             complement(319..444)
                     /locus_tag="ACHOQQ_RS04875"
                     /old_locus_tag="pgaptmp_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469259.1"
                     /translation="MSIFLSWVILIISVLMAIGIMRIIHTLKDIELFFTGDKRRK"
     gene            complement(441..683)
                     /locus_tag="ACHOQQ_RS04880"
                     /old_locus_tag="pgaptmp_000976"
     CDS             complement(441..683)
                     /locus_tag="ACHOQQ_RS04880"
                     /old_locus_tag="pgaptmp_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HGU4469260.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTKDESLRNSLRGAIMGLYN
                     CALGSDDREALEELKSIMTELFEGEIVEPYFTGVAA"
     gene            complement(702..1037)
                     /locus_tag="ACHOQQ_RS04885"
                     /old_locus_tag="pgaptmp_000977"
     CDS             complement(702..1037)
                     /locus_tag="ACHOQQ_RS04885"
                     /old_locus_tag="pgaptmp_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HGU4469261.1"
                     /translation="MKQQRNARINNNTERHVARQHFRKNSATVTTEIHATPDGHPVKQ
                     TGKHTWAIGDTGIVIHKSFRSPVTGRFNFTLTRGDDYFGQDFTFFEALRTADRLISGL
                     RFQYPGSKH"
     gene            complement(1063..1257)
                     /locus_tag="ACHOQQ_RS04890"
                     /old_locus_tag="pgaptmp_000978"
     CDS             complement(1063..1257)
                     /locus_tag="ACHOQQ_RS04890"
                     /old_locus_tag="pgaptmp_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469262.1"
                     /translation="MTDSTQTKDLYSDLRFIIIQQQSGRMDPTRASKAWKEALTDQGI
                     DVISEVMAEVIFRLTRDESL"
     gene            1256..1693
                     /locus_tag="ACHOQQ_RS04895"
                     /old_locus_tag="pgaptmp_000979"
     CDS             1256..1693
                     /locus_tag="ACHOQQ_RS04895"
                     /old_locus_tag="pgaptmp_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469263.1"
                     /translation="MSIPSNLDVFNETVGKTFAFLYETFPRKVTIDICALTGVSELTF
                     EEDRSSRRQLVEALELRRDSIAWLVDAGYVSAKSSPPHYFSDAVLTAKGLELLKLDPE
                     SLKQSFGDKLVDAAKSGSMDAIKSTASSALTTGLSFAFKSLLG"
     gene            complement(1775..2200)
                     /locus_tag="ACHOQQ_RS04900"
                     /old_locus_tag="pgaptmp_000980"
     CDS             complement(1775..2200)
                     /locus_tag="ACHOQQ_RS04900"
                     /old_locus_tag="pgaptmp_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HGU4469264.1"
                     /translation="MQEVTQNAPESPAETQCDQLTDAPETAHQKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGLRNKRISKKAA"
     gene            2308..2607
                     /locus_tag="ACHOQQ_RS04905"
                     /old_locus_tag="pgaptmp_000981"
     CDS             2308..2607
                     /locus_tag="ACHOQQ_RS04905"
                     /old_locus_tag="pgaptmp_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4469265.1"
                     /translation="MSTNVGEKIRIMRKSEMLTQEKMAEITGLTVAALRQYEQGRNEP
                     NLESTKKLLKSPVFRKYRDWFLFDEVEPKAGQVVPALAHIGQSEAESSHSGKKTG"
     gene            2674..3669
                     /locus_tag="ACHOQQ_RS04910"
                     /old_locus_tag="pgaptmp_000982"
     CDS             2674..3669
                     /locus_tag="ACHOQQ_RS04910"
                     /old_locus_tag="pgaptmp_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4469266.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKDLESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIITLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            3959..4210
                     /gene="chpS"
                     /locus_tag="ACHOQQ_RS04915"
                     /old_locus_tag="pgaptmp_000983"
     CDS             3959..4210
                     /gene="chpS"
                     /locus_tag="ACHOQQ_RS04915"
                     /old_locus_tag="pgaptmp_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HGU4469267.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            4204..4554
                     /gene="chpB"
                     /locus_tag="ACHOQQ_RS04920"
                     /old_locus_tag="pgaptmp_000984"
     CDS             4204..4554
                     /gene="chpB"
                     /locus_tag="ACHOQQ_RS04920"
                     /old_locus_tag="pgaptmp_000984"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HGU4469268.1"
                     /translation="MVKKSEFERGDIVLVGFDPASSHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(4634..5164)
                     /gene="ppa"
                     /locus_tag="ACHOQQ_RS04925"
                     /old_locus_tag="pgaptmp_000985"
     CDS             complement(4634..5164)
                     /gene="ppa"
                     /locus_tag="ACHOQQ_RS04925"
                     /old_locus_tag="pgaptmp_000985"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGU4469269.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            5474..6430
                     /gene="ytfQ"
                     /locus_tag="ACHOQQ_RS04930"
                     /old_locus_tag="pgaptmp_000986"
     CDS             5474..6430
                     /gene="ytfQ"
                     /locus_tag="ACHOQQ_RS04930"
                     /old_locus_tag="pgaptmp_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGU4469270.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            6570..8072
                     /gene="ytfR"
                     /locus_tag="ACHOQQ_RS04935"
                     /old_locus_tag="pgaptmp_000987"
     CDS             6570..8072
                     /gene="ytfR"
                     /locus_tag="ACHOQQ_RS04935"
                     /old_locus_tag="pgaptmp_000987"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HGU4469271.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQPISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIEMLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            8086..9108
                     /gene="ytfT"
                     /locus_tag="ACHOQQ_RS04940"
                     /old_locus_tag="pgaptmp_000988"
     CDS             8086..9108
                     /gene="ytfT"
                     /locus_tag="ACHOQQ_RS04940"
                     /old_locus_tag="pgaptmp_000988"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGU4469272.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            9095..10054
                     /gene="yjfF"
                     /locus_tag="ACHOQQ_RS04945"
                     /old_locus_tag="pgaptmp_000989"
     CDS             9095..10054
                     /gene="yjfF"
                     /locus_tag="ACHOQQ_RS04945"
                     /old_locus_tag="pgaptmp_000989"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HGU4469273.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLISEESIPINHPVYDTLSSLVWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQVGYALAGVGVELDAIASVVIGGTLFGVAIQGLIQTYINFDGTL
                     SSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(10087..11085)
                     /gene="fbp"
                     /locus_tag="ACHOQQ_RS04950"
                     /old_locus_tag="pgaptmp_000990"
     CDS             complement(10087..11085)
                     /gene="fbp"
                     /locus_tag="ACHOQQ_RS04950"
                     /old_locus_tag="pgaptmp_000990"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGU4469274.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            11261..12634
                     /gene="mpl"
                     /locus_tag="ACHOQQ_RS04955"
                     /old_locus_tag="pgaptmp_000991"
     CDS             11261..12634
                     /gene="mpl"
                     /locus_tag="ACHOQQ_RS04955"
                     /old_locus_tag="pgaptmp_000991"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGU4469275.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(12790..13341)
                     /gene="yjgA"
                     /locus_tag="ACHOQQ_RS04960"
                     /old_locus_tag="pgaptmp_000992"
     CDS             complement(12790..13341)
                     /gene="yjgA"
                     /locus_tag="ACHOQQ_RS04960"
                     /old_locus_tag="pgaptmp_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGU4469276.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            13435..14787
                     /gene="pmbA"
                     /locus_tag="ACHOQQ_RS04965"
                     /old_locus_tag="pgaptmp_000993"
     CDS             13435..14787
                     /gene="pmbA"
                     /locus_tag="ACHOQQ_RS04965"
                     /old_locus_tag="pgaptmp_000993"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGU4469277.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            14971..15357
                     /gene="cybC"
                     /locus_tag="ACHOQQ_RS04970"
                     /old_locus_tag="pgaptmp_000994"
     CDS             14971..15357
                     /gene="cybC"
                     /locus_tag="ACHOQQ_RS04970"
                     /old_locus_tag="pgaptmp_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4469278.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15402..15866)
                     /gene="nrdG"
                     /locus_tag="ACHOQQ_RS04975"
                     /old_locus_tag="pgaptmp_000995"
     CDS             complement(15402..15866)
                     /gene="nrdG"
                     /locus_tag="ACHOQQ_RS04975"
                     /old_locus_tag="pgaptmp_000995"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGU4469279.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16056..18194)
                     /gene="nrdD"
                     /locus_tag="ACHOQQ_RS04980"
                     /old_locus_tag="pgaptmp_000996"
     CDS             complement(16056..18194)
                     /gene="nrdD"
                     /locus_tag="ACHOQQ_RS04980"
                     /old_locus_tag="pgaptmp_000996"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGU4469280.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(18588..20243)
                     /gene="treC"
                     /locus_tag="ACHOQQ_RS04985"
                     /old_locus_tag="pgaptmp_000997"
     CDS             complement(18588..20243)
                     /gene="treC"
                     /locus_tag="ACHOQQ_RS04985"
                     /old_locus_tag="pgaptmp_000997"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGU4469281.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPEQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(20293..21714)
                     /gene="treB"
                     /locus_tag="ACHOQQ_RS04990"
                     /old_locus_tag="pgaptmp_000998"
     CDS             complement(20293..21714)
                     /gene="treB"
                     /locus_tag="ACHOQQ_RS04990"
                     /old_locus_tag="pgaptmp_000998"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGU4469282.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(21833..22780)
                     /gene="treR"
                     /locus_tag="ACHOQQ_RS04995"
                     /old_locus_tag="pgaptmp_000999"
     CDS             complement(21833..22780)
                     /gene="treR"
                     /locus_tag="ACHOQQ_RS04995"
                     /old_locus_tag="pgaptmp_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HGU4469283.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            22965..23018
                     /gene="mgtL"
                     /locus_tag="ACHOQQ_RS05000"
                     /old_locus_tag="pgaptmp_001000"
     CDS             22965..23018
                     /gene="mgtL"
                     /locus_tag="ACHOQQ_RS05000"
                     /old_locus_tag="pgaptmp_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGU4469284.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            23159..25855
                     /gene="mgtA"
                     /locus_tag="ACHOQQ_RS05005"
                     /old_locus_tag="pgaptmp_001001"
     CDS             23159..25855
                     /gene="mgtA"
                     /locus_tag="ACHOQQ_RS05005"
                     /old_locus_tag="pgaptmp_001001"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGU4469285.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(26061..26447)
                     /gene="ridA"
                     /locus_tag="ACHOQQ_RS05010"
                     /old_locus_tag="pgaptmp_001002"
     CDS             complement(26061..26447)
                     /gene="ridA"
                     /locus_tag="ACHOQQ_RS05010"
                     /old_locus_tag="pgaptmp_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGU4469286.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(26520..26981)
                     /gene="pyrI"
                     /locus_tag="ACHOQQ_RS05015"
                     /old_locus_tag="pgaptmp_001003"
     CDS             complement(26520..26981)
                     /gene="pyrI"
                     /locus_tag="ACHOQQ_RS05015"
                     /old_locus_tag="pgaptmp_001003"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGU4469287.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(26994..27929)
                     /gene="pyrB"
                     /locus_tag="ACHOQQ_RS05020"
                     /old_locus_tag="pgaptmp_001004"
     CDS             complement(26994..27929)
                     /gene="pyrB"
                     /locus_tag="ACHOQQ_RS05020"
                     /old_locus_tag="pgaptmp_001004"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGU4469288.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(27933..28067)
                     /gene="pyrL"
                     /locus_tag="ACHOQQ_RS05025"
                     /old_locus_tag="pgaptmp_001005"
     CDS             complement(27933..28067)
                     /gene="pyrL"
                     /locus_tag="ACHOQQ_RS05025"
                     /old_locus_tag="pgaptmp_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGU4469289.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(28348..28743)
                     /gene="yjgH"
                     /locus_tag="ACHOQQ_RS05030"
                     /old_locus_tag="pgaptmp_001006"
     CDS             complement(28348..28743)
                     /gene="yjgH"
                     /locus_tag="ACHOQQ_RS05030"
                     /old_locus_tag="pgaptmp_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4469290.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGS
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDNIIDITSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(28874..29587)
                     /gene="bdcA"
                     /locus_tag="ACHOQQ_RS05035"
                     /old_locus_tag="pgaptmp_001007"
     CDS             complement(28874..29587)
                     /gene="bdcA"
                     /locus_tag="ACHOQQ_RS05035"
                     /old_locus_tag="pgaptmp_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4469291.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDHL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            29658..30251
                     /gene="bdcR"
                     /locus_tag="ACHOQQ_RS05040"
                     /old_locus_tag="pgaptmp_001008"
     CDS             29658..30251
                     /gene="bdcR"
                     /locus_tag="ACHOQQ_RS05040"
                     /old_locus_tag="pgaptmp_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4469292.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            30396..30848
                     /gene="tabA"
                     /locus_tag="ACHOQQ_RS05045"
                     /old_locus_tag="pgaptmp_001009"
     CDS             30396..30848
                     /gene="tabA"
                     /locus_tag="ACHOQQ_RS05045"
                     /old_locus_tag="pgaptmp_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGU4469293.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            30971..>31481
                     /gene="yjgL"
                     /locus_tag="ACHOQQ_RS05050"
                     /old_locus_tag="pgaptmp_001010"
     CDS             30971..>31481
                     /gene="yjgL"
                     /locus_tag="ACHOQQ_RS05050"
                     /old_locus_tag="pgaptmp_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HGU4469294.1"
                     /translation="MSKISDLNYSQHITSADNFKPKNEVLNTWCVGMNDFARIAGGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADSSKVIIEISLSTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDDS
                     SLHLITNKVL"
BASE COUNT         7472 a         7731 c         8315 g         7963 t
ORIGIN      
        1 cagatatgtg cacgctcggc ttttcttgcg gccatgctga tatgcgcatg atggttagct
       61 ggtctggcgg ttatttctct gccgggtgat tcggctgttc tcatttgttg actcctaaaa
      121 aaaagacaaa gggaacccga aggcatgaat gccttaatta attgctcgtg tgttttctgg
      181 tgttattgcg ttcttgtttg cagcttcagt ttatccacag attttcaggg ctaaccaggt
      241 gaggtcacac accataaata tcacccatac agtggcgatg gtgagtattg cttttacatt
      301 cttcataaag tttttgcttt atttacggcg tttatcgccg gtaaagaata attcgatatc
      361 cttcagtgtg tggataattc gcataatgcc gattgccata agcacggaga taatcagaat
      421 tacccatgaa aggaatatgc tcatgcggct acccctgtga agtaaggctc gacaatttca
      481 ccttcaaaca gttctgtcat aatcgacttt aattcttcca gcgcttcacg gtcatcgctg
      541 cccagtgcgc agttgtatag ccccataatt gcgccgcgca gtgagttacg aaggctttca
      601 tccttagtta cgccccggtt tgcgagttca aagtgataac gttgcaacgc gttattgata
      661 agtattttgt aggcttcgat catggctgaa tcctttattt atcagtgctt gcttcccgga
      721 tattgaaaac gcaggccgct gattaatctg tctgccgttc ttaaggcttc aaaaaatgtg
      781 aagtcctgtc cgaagtaatc atccccgcga gtcagggtga aattaaatct gccagtaaca
      841 gggctgcgaa aacttttgtg aataacgatg ccagtgtcgc caatcgccca ggtgtgcttt
      901 ccggtttgtt ttaccgggtg accgtctggt gtggcatgga tttcagtggt tacggtcgca
      961 ctatttttac ggaagtgctg gcgtgcaacg tggcgttcgg tgttgttatt gatgcgtgca
     1021 tttcgttgtt gcttcattct tcactcggtc cttatgcgat gattacagtg attcgtctct
     1081 tgtcagccta aaaatcactt cagccatgac ttcgctaatt acatcgatcc cctgatcggt
     1141 aagggcttct ttccaggcct tgcttgcacg tgttggatcc atcctgcctg attgctgttg
     1201 tatgatgatg aagcgtaaat cgctgtataa gtcttttgtt tgagtggagt cagtcatgtc
     1261 aataccttca aatcttgatg tttttaatga aacagttggt aaaacttttg cgtttcttta
     1321 tgaaactttt ccccgcaaag taactattga tatttgtgcc ttaaccggtg tttcagagct
     1381 gacttttgaa gaagaccgat cctccaggag gcaactagtt gaagctcttg agcttcgccg
     1441 tgactccatc gcatggcttg ttgatgctgg ttatgtatcc gctaaatctt ctcctccgca
     1501 ttacttttct gatgcggttc tcacggccaa aggtcttgaa ttacttaagc ttgatccaga
     1561 gtcacttaaa caatcatttg gcgacaagct tgttgatgca gccaagagtg gttcaatgga
     1621 tgcgataaaa agcaccgcat catccgctct tactactggg ttatcctttg ctttcaaaag
     1681 tcttcttggt tgattgttgc gcccccgcat aacaccaacg attagattat ttacggcgaa
     1741 atctcctgtt gtggatttcg ctgtgtgttt gatattaagc tgctttttta ctgatacgtt
     1801 tattccgtaa accagctgct ggcttaaata aaaaaagtcg gttttcccat tcgtgccatt
     1861 cttgcggtgc gttttcagtg agtcgatcgc aggcttcatc ccacgctttc cggttgatgt
     1921 agagttcggc gtttccgcca ggcttgagag ggtccgccat gtacacagcg ggtaatttcc
     1981 ctgcttttgc catgctcacg atggcgtcaa cggtcttgcc gatatacatc gcaaaccctt
     2041 cttttgacag gagatgagaa gggttttgtg aaagcctgat ttcctggcgt tcaggggctt
     2101 tgcgcccggt cttttggtgt gcggtttctg gcgcatctgt caactgatcg cactgtgttt
     2161 cggcaggcga ttcaggcgcg ttttgagtga cttcttgcat ctggtatcct ccgttattgg
     2221 tgctatttct atttagcacc agttagagcc gcctagattg tctctaggtg tgtttgttga
     2281 tgtataaact aagagagtta actcactatg tcaactaacg taggtgaaaa aattagaatc
     2341 atgcgtaagt ctgaaatgct tacacaagaa aaaatggctg aaattacagg gctcacggta
     2401 gcggccctgc gtcaatatga acaggggaga aatgagccaa atcttgaatc cacaaaaaag
     2461 ctgctcaagt ctcccgtctt ccgtaaatac cgtgactggt ttttgttcga tgaggtcgaa
     2521 ccaaaggccg ggcaggtggt accggctctc gcacacattg ggcaaagcga agcggaatca
     2581 tcccactccg gcaagaagac tggttaacgg tccatctggc ctttatttct caaaattgtg
     2641 atcaagatga tgttttcatt ggggggcttc cttatgtcga ttaagaagct cgctgatggc
     2701 aaatactgtg tggacgtgcg gcctgctgga tctgaaggaa gaagatttcg gcggcgattt
     2761 cctacccgtg gtgaagcggc gatgtatgaa cgccatgttc tgacgcatta tcacgataag
     2821 gactgggtcg aaaaacctat tgaacgtaaa gcgttaagcg atcttctgga actgtggtgg
     2881 gtgtaccacg gtaagaatca tcgttatggc gcgatgaatc gggtcagaat tgaagcggtt
     2941 cttcgtgata tgcaagagct tggtattaac agaagtgacc acttaacccg aaaaaatatt
     3001 atcagatatc gccttgaatt aatgaatcgc ggaattaagc agtcaacggt aaatcgttat
     3061 tgtgcaatga tgagcggttt ctttgaaaag ctgattgatg ttgaagagtt tgtgggtgat
     3121 aacccgtttc acgagatacg caagctaaca attaatcagc cggaaatggc ttatcttgca
     3181 catgatgata ttcccaggct aaccgcactt ttatctggcg ataatcttaa ggctgtattg
     3241 ttaagcctgg ctacaggcgg gcgctggggt gaagttgcca atattaaagg tgaacacata
     3301 attggtggga aggttatttt catggagaca aagaatggat cgcggcgggt tattcctata
     3361 gctaaagatc ttgaatctct gattaaagtc aaagcgacgg gaagattaat gaaccccagt
     3421 tatgcgatag tcagaagtgc aataaggact gtgcggcctg atttaccagt cggtcagtct
     3481 gtgcatgttt tacggcacac attcgcaact catttcatga taaatggagg gaacattatt
     3541 acccttcagc gtattttagg gcacagcaca attcagcaga caatgaccta tgcgcacttt
     3601 gcacctgatt atctccagga cgcggtaagg ttcaatccgg ttgcggagtt gtccagattt
     3661 tgcccataaa cggggactaa atggcactat tccgaactac tttgaactcg taactatttg
     3721 atatataaag ggggatagcg atacgcgcta cccccttatg ccaccttcgg gtggcgttgt
     3781 tttttgcgag acgactcgca ttctgttttg taattccctc accttttgct tttctctccg
     3841 agccgctttc catatctatt aacgcataaa aaactctgct ggcattcaca aatgcgcagg
     3901 ggtaaaacgt ttcctgtagc accgtgagtt atactttgta taacttaagg aggtgcagat
     3961 gcgtattacc ataaaaagat gggggaacag tgcaggtatg gtcattccca atatcgtaat
     4021 gaaagaactt aacttacagc cggggcagag cgtggaagcg caggtgagca acaaccaact
     4081 gattctgaca cccatctcca ggcgctactc gcttgatgaa ctgctggcac agtgtgacat
     4141 gaacgccgcg gaacttagcg agcaggatgt ctggggtaaa tccacccctg cgggtgacga
     4201 aatatggtaa agaaaagtga atttgaacgg ggagacattg tgctggttgg ctttgatcca
     4261 gcaagcagcc atgaacagca aggtgctggt cgacctgcgc ttgtgctctc cgttcaagcc
     4321 tttaatcaac tgggaatgac gctggtggcc cccattacgc agggtggaaa ttttgcccgt
     4381 tatgccggat ttagcgttcc tttacattgc gaagaaggcg atgtgcacgg cgtggtgctg
     4441 gttaatcagg tgcggatgat ggatctacgc gcccggctgg caaagcgtat tggtctggct
     4501 gcggatgagg tggtggaaga ggcgttatta cgcttgcagg cggtggtgga ataaggtgtg
     4561 tttatttatc gcgggcataa aaaaaccctt actaaccgaa gcccggcgtt cagggttatt
     4621 gcgctagaag aacttattta ttcttggcgc gctcgaagga ggcaacgatt tcagctttag
     4681 cggcttctgc gttttcccaa ccttcaactt tcacccactt gcctttttcg aggtctttgt
     4741 agtgctcgaa gaagtgagcg atctgcgctt tcagcagctc tggcagatcg ttaacgtctt
     4801 taatgtgatc gtattctttg ctcagcttgc tgtgcggaac cgcaaccagt ttcgcatctt
     4861 caccggcttc gtcggtcatt ttcagaacgc caaccggacg gcaacggatc acagaacccg
     4921 gctgcagcgg gtacggagtc gggaccagta cgtcaaccgg gtcaccgtcc agagacaggg
     4981 tgtggttgat gtaaccgtag ttgcatggat agaacatcgc ggtggacatg aagcggtcaa
     5041 cgaacagtgc gccgctctct ttgtcgattt cgtatttgat cggatctgcg ttagccggga
     5101 tctcaataac aacgtagatg tcttccggca gatctttacc cgcagggacg ttgagtaagc
     5161 tcatgtctgt ttcctttaaa aatatgtggc aaacaagtgc cgagtattat agccaactcg
     5221 cgccgaatgt cttcgcttgt tttcgtcttc attttccttt ttcgccagtt ttaagacggt
     5281 atacagagca ggaaaatcca taaccgtagc cgcatttttc atagtgagat gaaagcgatt
     5341 acaaacttgt gattaacgtt ttatttactt ttttgaagtg tgatgtaacg caatccgtta
     5401 cataacgaat tgtctatagt tttttcgcga acatcttttc accaataata attaccccga
     5461 cgaggacaac cctatgtgga aacgcttact tgtagtctct gcagtctcgg cagccatgtc
     5521 gtctatggcg ttggccgctc cattaaccgt aggattttcg caggtcggat cggaatccgg
     5581 ctggcgcgcc gcagaaacca atgtggcgaa aagtgaggcc gaaaaacgcg gaattacgct
     5641 gaaaattgcc gatggtcagc aaaagcagga aaaccagatt aaagcggtac gttccttcgt
     5701 tgcacaaggg gtggatgcga tctttatcgc tccggtggtt gcgacgggtt gggaaccggt
     5761 attaaaagag gcgaaagatg ccgaaatccc ggtcttcttg ctcgaccgtt ccatcgatgt
     5821 gaaagacaaa tctctctata tgaccaccgt tactgccgac aacatcctcg aaggcaagtt
     5881 gattggtgac tggctggtaa aagaagtgaa tggcaaacca tgcaacgtgg tggagctgca
     5941 gggcaccgtt ggggccagcg tcgccattga ccgtaagaaa ggctttgccg aagccattaa
     6001 gaatgcgcca aatatcaaaa tcatccgctc acagtcaggt gacttcaccc gcagtaaagg
     6061 caaagaagtc atggagagct ttatcaaagc ggaaaacaac ggcaaaaaca tctgcatggt
     6121 ttacgcccat aacgacgaca tggtgattgg tgcaattcag gcaattaaag aagcgggcct
     6181 gaaaccgggc aaagatatcc tcacgggttc cattgacggt gtaccggaca tctacaaagc
     6241 gatgatggat ggcgaagcga acgccagtgt tgaactgacg ccgaacatgg cgggacccgc
     6301 ttttgacgcg ctggagaaat acaaaaaaga cggcaccatg cctgaaaagc tgacgttaac
     6361 caaatccacc ctttacctgc ctgataccgc aaaagaagag ttagagaaga agaaaaatat
     6421 ggggtattga gggttgctat gcctgatgcc gattcgtagg ccggataagg cgctcgcgcc
     6481 gcatccggcg atggtgcact gaagcctgat gcgacgctta ccgcgtctta tcatgcctac
     6541 tgggagcacg ctttacaccg ggggaaacca tgacgaccga ccaacaccag gagatcctcc
     6601 gcaccgaagg attaagtaaa tttttccccg gcgtcaaagc gttagacaac gttgatttca
     6661 gcctgcgccg tggcgaaatc atggcgctgc tcggtgaaaa cggggcggga aaatcaacgc
     6721 taatcaaagc attaactggt gtataccatg ccgatcgcgg caccatctgg ctggaaggcc
     6781 agcctatctc accgaaaaat accgcccacg cgcaacaact cggcattggc accgtctatc
     6841 aggaagtaaa cctgctaccc aatatgtcgg tcgctgataa tctatttata ggccgcgaac
     6901 ccaaacgctt cggccttcta cgccgcaaag agatggaaaa gcgcgccacc gaactgatgg
     6961 catcttacgg tttctccctc gacgtgcgcg aaccgctcaa ccgcttttca gtcgcgatgc
     7021 agcaaatcgt cgctatttgc cgggctatcg atctctctgc caaagtgctg atcctcgatg
     7081 aaccaaccgc cagtctcgac acccaggaag tggagttact gtttgacctg atgcgtcagt
     7141 tgcgcgatcg cggcgtcagc ctgatttttg tcactcactt tctcgatcag gtctatcagg
     7201 tcagcgatcg gatcaccgtc ttacgcaacg gcagtttcgt aggctgtcgg gaaacgtgcg
     7261 agctaccgca gatcgaactg gtaaaaatga tgctggggcg cgagctggat acccacgcgc
     7321 tacagcgtgc cgggcgaaca ttgttgagcg acaaacccgt tgccgcgttc aaaaattacg
     7381 gcaaaaaagg aacgatcgca ccgtttgatc tcgaagtacg ccccggcgag atcgtcggtc
     7441 tggctggctt gctgggatca ggacgtaccg aaaccgccga agtgatcttc ggtatcaaac
     7501 ctgctgacag cggcactgcg ttgatcaaag gcaaaccgca aaccctgcga tcgccacatc
     7561 aggcgtcggt actgggtatc ggcttctgcc cggaagacag gaaaaccgat ggcatcatcg
     7621 ccgctgcctc ggtgcgggaa aatatcatcc tcgcgctaca ggcccagcgc ggctggctgc
     7681 gaccgatctc ccgcaaagaa cagcaagaga ttgccgaacg ctttatccgc cagcttggca
     7741 ttcgcacccc ttccactgaa caaccgattg aatttctctc cggcggcaat cagcaaaaag
     7801 tgctgctctc tcgctggctg ctgactcgtc cgcaatttct gatcctcgac gagccaacgc
     7861 gtggaattga cgttggtgcg cacgccgaga tcatccgcct gattgaaatg ctatgcgccg
     7921 atggtctggc gctgctggtg atctcctccg aactggaaga actggtgggc tatgccgacc
     7981 gggtgatcat catgcgcgat cgcaaacagg tggcggagat cccgctggca gagctttccg
     8041 ttccggcgat catgaacgcc attgcggcgt aaggagaaca gtgtgatgcc tcaatctctt
     8101 ccggacacca ctacgccaaa aaggcgcttt cgctggccga cggggatgcc gcagctggta
     8161 gcactattgc tggtgctgct ggtcgatagc ctggtagccc cgcatttctg gcaggtggtg
     8221 cttcaggatg ggcgtttgtt cggtagcccc atagacattc ttaaccgtgc agctcccgtt
     8281 gcgttactgg cgattggcat gacgctggtg atcgccaccg gtgggattga tctctccgta
     8341 ggggcggtga tggctatcgc cggagccaca acggctgcga tgacggtcgc gggattcagc
     8401 ctgccgattg ttttgttaag cgccctgggc accggcatcc tggcgggatt gtggaacggc
     8461 atactggtag cgatcctcaa aattcagccg tttgtcgcca ctctgatcct gatggtcgcc
     8521 gggcgcggcg tggcgcaact gatcaccgcc gggcagatcg tcacgtttaa ctcgccggat
     8581 ctctcatggt tcggcagtgg atcgctgttg ttcctgccaa cgccggtcat tatcgcggtg
     8641 ctgacgctta tcctgttctg gctgttgacc cgcaaaacgg cgctggggat gtttatcgaa
     8701 gccgttggta tcaacattcg ggcggcaaaa aatgccgggg taaacacgcg gatcatcgtc
     8761 atgcttacct acgtgttgag cgggctgtgt gcggcgattg cgggcattat cgtggcggcg
     8821 gatattcgcg gtgccgatgc caataacgcc gggttatggc tggagctgga cgccattctc
     8881 gcggtggtga ttggcggcgg atcgctgatg ggcgggcgct ttaacctgct actttcggtg
     8941 gtgggggcgc tgattattca ggggatgaac accggaattt tgctttcggg ctttccgcca
     9001 gagatgaacc aggttgtaaa agcggtggtg gttctttgcg tgctgattgt ccagtcgcaa
     9061 cgctttatca gtctgattaa aggagtacgt agccgtgata aaacgtaatt tgccactgat
     9121 gatcaccatc ggcgtctttg tgttgggtta tctttactgc ctgacccagt ttcccggttt
     9181 tgcttccaca agagtgatct gcaatatcct gaccgataac gcctttcttg ggatcattgc
     9241 cgttggcatg acctttgtga tcctctccgg tgggatcgat ctctccgtcg gttcggtgat
     9301 cgcctttacc ggcgtgtttc tggcaaaagt gattggagat ttcggtctct caccgctgct
     9361 ggcgtttccg ctggtgctgg tgatgggctg tgccttcggc gcatttatgg ggcttttgat
     9421 cgacgccctg aagatcccgg catttatcat tacccttgca gggatgttct ttttgcgcgg
     9481 tgtcagctat ctcatctcag aagagtcgat cccgataaac catcctgttt atgacacgct
     9541 ctcaagcctt gtatggaaaa tccctggcgg cggtcgctta agtgcgatgg ggctgctgat
     9601 gctggcggtg gtggttatcg gcatattcct cgcgcatcgt acccgttttg gtaatcaggt
     9661 atacgccatt ggcggcaatg cgacgtcggc aaacctgatg gggatttcta cccgtagcac
     9721 tactattcgc atttatatgc tctccaccgg actggcaacg ctggcgggga ttgtcttctc
     9781 gatttatacc caggtcggat atgcgctggc gggcgtaggt gtggaactgg acgctatcgc
     9841 ctcagtggtg attggcggta cgctttttgg cgtggcgatt cagggactga ttcagactta
     9901 cataaacttt gatggcacgc tgagctcatg gtggacgaaa atcgccatcg gtattttgtt
     9961 gtttattttt atagcattac agcgtgggtt aacggtgctg tgggagaatc gtcagagttc
    10021 gccagtgaca agagtcaaca ttgcgcagca ataaaaacgc ctctccgtgt ggagaggcgc
    10081 aggagattac gcgtccggga actcacggat aaagcgttcg acatcttcaa ccatatggtc
    10141 gttgccgaca aagaacgaac ggcgctggtg cagggtttcc gggataatat ccagaatacg
    10201 ctctttgcca tcgctcgctt taccgcccgc ttgttccgcc aggaatgcca tcgggttgca
    10261 ctcatacagc aaacgcagtt tgccgtccgg gtggctggcg gtgcttgggt agagataaat
    10321 accgcctttc agtaggttac ggtggaaatc cgcaaccagt gagccgatat aacgtgaggt
    10381 atacgggcgg ttggtggatt tatcttcttc ctggcagaat ttaatgtact tcttcacccc
    10441 gttcggaaac ttaatgtagt ttccttcgtt gatggagtag gttttgcctt tctccgggaa
    10501 gcgcatccgt tcctggcaca ggcagaaaac gccgagcgaa ggatcgtaag taaaggcgtg
    10561 aacaccgcat ccggtggtgt aaaccagcat ggtagaggag ccgtatacca cgtaacctgc
    10621 cgcaacctgt ttgttaccag gctggaggaa atcttcttcc gttaccggcg tgccaacagg
    10681 cgtaacgcgg cggtagatgg agaaaatggt accgacagag acgttaacat cgatgttgga
    10741 cgagccatcc agggggtcca tcagcaccac gtattttgcg tgttcacagc cttcaaagac
    10801 gacaatctca tcttcttctt cagaggcaat gcccgcaacg atatcgcgtg ctttcagtgc
    10861 ggctttcagt ttttcattag cgaacaagtc gagtttctgt tgaacctcgc cctgcacgtt
    10921 ctcagcaccg ctggcaccca ggatatcaac cagtcctgct ttgttgatat cacgatggat
    10981 aatcttggcg cccagtttta ttgccgacag caaagcagtg agctcaccgg tagcatgaga
    11041 aaactcgtgc tgcttttcga caataaattc acctaacgtt ttcataaaac tttccctgca
    11101 atgtttatgg agtaaagcga ccgcaacaat cttaacaaat aatctcaatg ttgcgctcag
    11161 gtgaatcgcg ccagcaaatt gcggattatc ctgaaatgcg tttctcactt gcccgacata
    11221 tgcgtaaaat gagcggcaga ttaaaaaagg atagtgacgt atgcgcattc atattttagg
    11281 aatttgtggc acgtttatgg gcggtctggc gatgctggcg cgccagttag gccatgaagt
    11341 aacgggttcg gacgccaatg tgtatccgcc gatgagcacc ttacttgaga agcaaggcat
    11401 tgaactgatt cagggttacg atgccagcca gctcgatccg cagccggatc tggtgattat
    11461 tggcaacgcc atgacccgtg gaaatccgtg tgtggaagcg gtactggaaa aaaacatccc
    11521 ttatatgtca ggtccacagt ggctgcacga ttttgtgctg cgcgaccgct gggtgctggc
    11581 cgttgccggt acacacggca aaaccaccac cgcgggaatg gcgacctgga ttctggaaca
    11641 gtgtggttac aaaccgggct ttgtaatcgg cggtgtgccg gggaactttg aggtttcggc
    11701 gcgtctgggc gaaagcgact tctttgttat cgaagcggat gagtacgact gcgccttctt
    11761 cgacaaacgc tctaaatttg tccattactg cccgcgtacg ctgatcctca acaaccttga
    11821 gttcgatcac gccgatatct ttgacgacct gaaagcgatc cagaaacagt tccaccatct
    11881 ggtgcgtatc gttccggggc agggccgtat tatctggcca gaaaacgaca tcaacctgaa
    11941 acagaccatg gcgatgggct gctggagcga gcaggagctg gtgggtgagc agggtcactg
    12001 gcaggcgaaa aagctgacca ccgatgcttc cgaatgggaa gttttgctgg atggcgaaaa
    12061 agtgggcgaa gtgaaatggt cgctggtagg cgaacataat atgcacaatg gcctgatggc
    12121 gattgcagcg gctcgccatg ttggtgtagc gccggcagat gccgctaacg cgctgggttc
    12181 gtttattaat gctcgtcgcc gtctggagtt gcgtggtgaa gcgaatggcg tcacggtata
    12241 tgacgatttt gcccatcacc cgacggcgat tctggcaacg ctggcggcgc tgcgtggcaa
    12301 agttggtggt acggcgcgca ttattgctgt gctggaaccg cgctcgaata ccatgaaaat
    12361 ggggatctgc aaagacgatt tggcaccttc tttaggtcgt gccgatgaag tcttcctgct
    12421 gcaaccggcg catattccgt ggcaggtggc agaagtggca gaagcctgcg ttcagcctgc
    12481 acactggagt ggcgatgtgg atacgctggc agatatggtg gtgaaaaccg ctcagcctgg
    12541 cgaccatatt ctggtgatga gcaacggcgg ttttggtggg atccatcaga aactgctgga
    12601 tggtctggcg aagaaagcgg aagccgcgca gtaattcggc cttagcctga tatagtgtgc
    12661 gtaggccgga taaggcgttc acgccgcatc cggcatttga gcatagtgcc tgatgcgacg
    12721 cttgatgcgt cttatcaggc ctacaggtgc accgcatccg gcaaaccata ctcacatcaa
    12781 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
    12841 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gagtacgcag
    12901 ttgctgacga tcggcatcgg gccacagatt taatacctca gcgatggcgt catcaccttg
    12961 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
    13021 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
    13081 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
    13141 aatagccgcg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
    13201 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
    13261 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
    13321 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
    13381 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
    13441 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
    13501 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
    13561 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagc
    13621 gatggcgcgc tggggatcac tgtttatcac cagaaccgca aaggtagcgc atcatccacc
    13681 gatttaagcc cgcaggccat tgcccgtact gtacaggcgg cgctggatat tgcccgttat
    13741 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcacca
    13801 gatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
    13861 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
    13921 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
    13981 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
    14041 cgtgattacg cctacaccat tggtcgtgcg atgagcgatc tgcaaacgcc agagtgggtt
    14101 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
    14161 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
    14221 gcgatagcgg gtggatcggt ttaccgtaaa tctaccttcc tgctggattc gctgggtaaa
    14281 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
    14341 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
    14401 atcctgactc agtggctgct gaccagctat tcggcgcgaa aactggggct gaaaagcacc
    14461 ggacatgcgg gcggtattca caactggcgg attgccggac aaggtctaag cttcgagcag
    14521 atgctcaaag agatgggcac cgggctggtg gtgacggaat tgatgggcca gggcgtgagt
    14581 gcaattaccg gtgattattc ccgtggtgcg gcgggcttct gggtagaaaa cggcgaaatt
    14641 cagtatccgg ttagcgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
    14701 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
    14761 ccggagatga aaatcgccgg acagtaaaaa aactggcgcg acctgccgcg ctataaacta
    14821 aaaaattaca caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag
    14881 cgtactcgca gtacgtgatc cggtcgaatg agcgtagtcg tcgatgaggc agtgtgaagg
    14941 atgaagtgta aataaaaaag gaagtgagca atgcgaaaaa gcctgttagc tattcttgca
    15001 gtctcctcgt tggtattcag ttcggcgtcg tttgccgctg atcttgaaga caatatggaa
    15061 accctcaacg acaatttaaa agtggtcgaa aaagccgata acgcggcgca agtcaaagac
    15121 gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc gccgaagctc
    15181 gaagataaat caccggacag cccggaaatg aaagatttcc gccacggttt cgacattctg
    15241 gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa agaagcgcag
    15301 gctgccgcag agcaactgaa aacgacccgc aacgcctatc accagaagta tcgttaattc
    15361 cttatttccc tgttgcctgc actcaggtaa cagggaactt atcatcgcaa atgatgcacc
    15421 acctgattgc tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg cacaaatttg
    15481 ccgtcgacca gcacgttaat caaatcaaca acctgcattt gcgcagcgtt gagttcgtcg
    15541 agtttatagc ctgtccacac ccagatgtct ttacccggac actcggcgcg gatgcgttgt
    15601 accagtttca gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc ggagagggaa
    15661 ataccctggc gtttgatacg agtgtcattc agatcgttaa tgatctggtc ttccattgct
    15721 ttagtaaatg gctgaccgga atttacccgc catgtgcttt tgttatagca accggggcat
    15781 tcatgaacac acccggagac aaacagggtg cagcgagtgc cgggaccgtt gacgatgtcg
    15841 acaggatagt actgatgata attcattatt tcgcctggct tatcagtccg cgtgtaggct
    15901 gtatcaggca atcaacgcct gatgcgacgc tgtcgcgtct tatcaggcct acagccgttg
    15961 ccaaatgtag gccggataag gcgtttacgc cgcatccggc aatcaacgcg ggatgcgacg
    16021 ctttcacatc acatccggca tttgcttagt gcagatcaac ctatctgccc atttcccaaa
    16081 tgtttaacgc ggcgcttaac ttcttcctgc ttaccagcgt taaacggacg tgcatccggg
    16141 ctacctaaat atccgcacac gcggcgagtt accgacacac gggaggcgtc atggttacca
    16201 catttcgggc aagtgaagcc tttgctggtg cactcgaact caccggtaaa gccacactcg
    16261 tagcactcat caatcggtgt attggtgccg taatacggta catgctgata gctgtaatcc
    16321 cagacatctt ccagcgcctt caggttgtgc tgaatgtttg gatactcgcc gtagcaaatg
    16381 aaaccaccgt tcgccagcgg cgggtaaggc gcttcaaagt cgatcttgtc gtacgggttc
    16441 accttcttct ccacatcgag gtggaaactg ttggtgtagt aacctttatc ggtcacgccc
    16501 ggcaccacgc caaactcagc agtatcgaga cggcagaagc ggtcgcacag gttttcactc
    16561 ggcgtgctgt agagactgaa accataaccc gtttcttctt tccactgatc cactgcctga
    16621 cgcagacgtt caacaatggc gatacctttc gcgcgaagct gctcgttgtc gtaaacatgc
    16681 tcgccgccga acagcgcgtt aatggtttcg tggatgccga tgtaacccag cgaaatagac
    16741 gcacgaccgt ttttgaagat ttcagaaaca tcatcgtcag cattcagacg cacgccacaa
    16801 gcaccttcca tatagaggat cggggccacg cgcgctttca cgccttcgag acgagcgata
    16861 cgggtcatca gcgccttacg tgccagcacc agacgttcat ccagcagctt ccagaaggtg
    16921 gcttcatcgc cttttgcttc cagagcaata cgcggcaggt tcaggctgat cacgccgagg
    16981 ttgttacgac catcgtggat ctgctcgccg ttttcatttt cccacacgcc gaggaagctg
    17041 cggcagccca tcggggtttt aaacgaaccg gtgactttca ctacctgatc gtagttcagg
    17101 atatccggat acatgcgctt gcttgcgcac tccagcgcca gctgtttgat gtcgtagttc
    17161 ggatcgcctt ttttatggtt caggccatcg cgaatcgcaa acaccagttt cgggaacacc
    17221 gcagttttac ggtttttacc gaggcctgcg atacggttac gcaggataga ttcctgaatc
    17281 aggcgcgact cccagctggt tcccagacca aaaccaaagg ttacaaacgg cgtctgaccg
    17341 ttggcggtgt gcagggtgtt tacttcgtac tccagcgact ggaaggcatc gtagcactct
    17401 ttaatggtac gagagttagc gtagccttcg gcgtccggga tgttccactc ttctgcggtc
    17461 ttgcgatgtt tgttgtagct ggcggtgaca aacggtgcca gcacttcatc gatacggtta
    17521 atggtggtgc cgccataaat atggctggcg acctgagcaa taatctgcgc agttacagcg
    17581 gttgccgtag agatcgactt cggcggttca atctcggcgt tccccatctt aaagccctgg
    17641 gtcagcatgc ctttcaggtc gatcaacatg cagttgaaca tcgggaagaa cggcgagtaa
    17701 tcgagatcgt gatagtgaat atcgccacgc tcatgtgcct gcaccacgtc acgcggcagc
    17761 aggtgctgac gtgcatagtg tttagccacg atcccggcca gcaggtcgcg ctgggttgga
    17821 atcaccttgc tgtctttgtt ggcgttttcg ttgagtaacg aggcgttggt ctgctcgacc
    17881 agaccacgga tctcctgatt caggcgaccg cgtttttcac gttcaatgtc gcgatcgtga
    17941 cggtactcga tgtaagcacg agccagttgt ttgtatggac ctgacatcag ctggttttca
    18001 acagcggtct ggatctcatt gatatccacc tggttgcggc cctgcatctg ctcgctgaca
    18061 actgcagcaa cagtggcgca ataatcggca tcatcgactt ccgctgcttt agctgcacgc
    18121 agaatcgctt ctttgatgcg ctctgattta aacggcactt tgcagccgtc tcgtttcatc
    18181 acatgcggtg tcatgatcgc tccatatttt taagaacagg ttatccacag aaattgggaa
    18241 aggcgttttc cggttttttc gtttcgtttt ccggtgcttt ccgcaatcgc catccgctta
    18301 tccacaacgc cgcacctttt tcgtggggag ctgtagtagc aattatagtc gattaataca
    18361 acatattggg ttgggacgca ttttaaagtc tatatatagt gctttgcatc aaggatgttt
    18421 gaactttttt tgatgtagct caaagtaaaa agcagagcgt acggatgacg ggcgctacag
    18481 cgttatgtaa attttttaat gaatttgctg gttaaaaaat caacaaaaac aacatactga
    18541 cagacaaaac cccggaatga ccggcattac cggggcttag ggaagattta cttctgtaac
    18601 caccagacag cctcaaaagg ccgtaaagtc atggcacagg gttgtggtga ggcttcttcg
    18661 tagttatgca tcacaagctg ccagttgccg cgcatttgct ctggctgcca gggttggatc
    18721 tcacggctaa ggttggcaat gaccagcaag gtttgcccct tccattcacg gcgatagcac
    18781 cacaatacag ggctgtttgg cagcagatcc tggtaattgc cccatgtcag aacggcttcc
    18841 tgcttacgca gtgcgattaa cttttggtag gtgtaaaaca ccgaggactc atcggccagc
    18901 gcggcttcta cgttgatttg ttgatagtta tcgcccaggc caatccacgg ttcgccagcc
    18961 gtaaacccgg cattatcgcc gttgctccat tgcatgggcg tgcggctgtt gtcacgggat
    19021 ttactggcga ggattgccaa taactcgtcg gcatcacgcc catcgttgcg cagctcggca
    19081 aacatattga ggctctctac gtcgcgatag tcagtaatgc gcgtgaaatg cgggttggtc
    19141 atgccaatct cttcgccctg gtagatatac ggcgttccct gcatgccatg cagcaccatc
    19201 gccagcattt ttgccgcagg cacgcggtat tcaccttcat caccaaagcg agaaacaatg
    19261 cgcggctgat cgtggttaca ccagaacaag gcattccatg ctacgttgtg cattccttgt
    19321 tgccagtggc ggaacaatgt tttcaacgcc acaaagtcag gtttagccag cgtccatttt
    19381 tcaccaccgg gataatcgac cttcaggtga tgaaaattaa aggtcatcga caattcactg
    19441 ccagtcagtg ccgcgtatcg ctggcaatgc tcaaggctgg tggaggacat ttcacctacg
    19501 gtcattaacc cgcgtggcgt aaacacatcg cggttcatct cgtgcaaaaa ctcgtgtgct
    19561 cgtggcccgt cggtgtagaa gcgacgcccg tcgccatcca ggtcatcagg gaaacgcggg
    19621 tctttggaga tcagattcac cacatccagg cgcaacccgt cgaccccacg atcggcccag
    19681 aactcacaga cttttttcag ctctgcgcgt accgctggat tctcccagtt gagatccgcc
    19741 tgttctggtg caaagagatg caaatagtac tgttcgcttt ccgcatgcca gcgccacgca
    19801 ctaccgccaa attttgaacg ccagttgttc ggtggcgttt ctggttctcc atcgcgccag
    19861 atataaaact ggcggtaagg gctttctttg ttcagcgcct cgcgaaacca ggcatgttgg
    19921 gtagaggtat ggttaaacac catatcgaga atgatacgaa tcccgcgcga ttttgcctgc
    19981 gtcaccagtt cgtcaaaatc gtccagcgtg ccgtaggtgg gatcaatcgc cgtatagttc
    20041 gctacgtcgt aaccgttatc gacctgggga gagacataaa agggggttag ccagatggca
    20101 tcaacgccca gtttatgcag atagtccagg cgttggataa cgccacgtaa atcgccggtg
    20161 ccgctacccg tggtgtcctg aaaactcttt ggataaatct ggtagataac gccgttttgc
    20221 caccagtggg gaagattagt cattacgtta ttcctgcaaa tgcgaggggg cgcaattgcg
    20281 ccccgaagaa aattaaacaa tgtccagcgt gcccaggcgg tatttccgct gatagataaa
    20341 cgaggtgagt acaatcggga tgatgatggc gatagccatt gccagcgcaa acacctgcca
    20401 gtagctcggt tgaatcgaga gaattcccgg caggccgcct acgccgatgc cattcgccat
    20461 aacgccgttc aggccgcata gcaatcctgc cagaccagaa ccaatcatcg cgcacagcat
    20521 cgggaagcga tatttcaggt tgatgccgta cattgcaggc tcagtgaccc caagccaggc
    20581 ggagatcgcc gcaggcacgg agatctcgcg ttcattgtgt ttgcggctgg aaatgataat
    20641 gcctatcacg gcggagccct gagcgatatt cgacagcgca atcagcggcc acactggcgt
    20701 accacccatg ctttgaatca tctgcaaatc aatagcaagc gtggtctggt gtaccccggt
    20761 gatcaccagc ggggcgtaca ggaagccaaa caatgccgcg ccaatcggag caaagctgcc
    20821 ggtcatcagg tgacgtaccg caaaggcaac gccatcgcca atcatgcgac caaacggacc
    20881 aatcagcgca tgggcgagga acaccgcgag gatcagcgaa cagacgggca ccaccaccag
    20941 atagaggtaa tctggcacga tgcgtttaag gcgagtttca ataacgccca gcgccagccc
    21001 ggctaatagt gccgggatca cctgcgcctg atagcccact ttggcgatgc tgaacatgcc
    21061 aaagtcccac acttccggca gctgctgccc gagcagataa gcgttcatca gctgggggga
    21121 aaccagtgtc acgccaagca cgataccaag gatcggcgtg ccgcccattt ttttcaccgc
    21181 tgaccagcaa atcccgaccg gcaggtagaa gaagatcgct tcaccgatca accacagaaa
    21241 atcgtagatc gtttgcaggg aagggtacat ttgcgccagc gtttgaccgt tgctcatggg
    21301 caaatcgccg atcacattgc gaaaaccgag gatcaaaccg ccgctaatca acgcgggcag
    21361 caacgggaag aagatctctg cgaaatgaga gatcaactgc tcatgccatt tcatattctg
    21421 ccgggcggct ttttttacct gctctttatc aacctgcgcc tgtccggttg acgctatcag
    21481 tgcttgatag taatcaccca cgttggtgcc aatcaccacc tgaaattgcc cggcattggt
    21541 gaaacagcct ttcaccatgg ggagttgctc aatttctttc ggtctggcat tggccggttg
    21601 gttgaggaca aagcgtaggc gagtaataca gtggctcacc gtcgcaatat tgccgcgccc
    21661 gccgaccagt tcaatcaacc gatcgatatc cgtttggttt attttgctca tcataaagcc
    21721 ccatggcaga tgacattttt ggttggctgc agaatatatt gcgcggaaaa atttaaaaac
    21781 gggaacgttc ccgaaacgca gcgaagatca caatttatcg ttcaggaaac gatcaggaca
    21841 gggtggcggg gatgatgatt tgttgcggtt cgctgcgtcc ggttacctgt gcgatcaact
    21901 ggcaggccgc ctggcgtcca gcttcggcgt aaccgggatc tacggtgacg atctccggat
    21961 ggaggaattt cattaacggc gtattaccga cgctcgccag ttgcaaggtg tcgatgcgtt
    22021 gctcttgcag gtatttactt gcgccaagtg ccagcgtgtc ggttgcgcac agtaaggcgg
    22081 tagtttcagg cgtaatcact tttgctacgt tctcatagcc ttgcttcata gcaaggcctg
    22141 gcagagcggc aacgggatgc agtttatgcg ctttgcagaa cgccaggtag gcttcgtgac
    22201 gtcgcttacc ggtcgtcacg tcactgtgcg gcacgccgag ataactgata ttacgatgcc
    22261 cctggtcata cagccgttgc atcaggatct tgattgcccc ttcgtcgtca taacagaccg
    22321 aagcaaagcc ttttgcgtca cgcgccagca gaaccagcga tgactgccag tgggctaaca
    22381 tttcttctgt tatgccagta aaaccgaaca gcactacgcc gtcgatatta cgccgtttca
    22441 gcacccccaa atgttcggca actaattgcg gggaaaactg actttccatc atgattgggt
    22501 cgtaaccttg ttcatagaac gctggcagca tggtttgaac ggcgagattt tctgacaacg
    22561 aatccagacg ggtaacaatg atggcgacca ctttatcgct ttgcccacgc atagcgcgcg
    22621 cagagcggga aggggaaaat ccatgctgat tcatcactgc ttcaacacgc tcgcgggtgc
    22681 gctggctcac gccgctttcg ttattcagca cccgggaaac tgtagatttc cccacgccgc
    22741 ttaagcgcgc gatgtctttg atggtcagcc gattttgcat cctgttgtcc tgtaacgtgt
    22801 tgtttaatta tttgagccta actttacccg tgcattcagc aatgggtaaa gtctggttta
    22861 tcgttggttt agttgccagc aggtattata tcgccataga tgctacgaat attattggat
    22921 tctccttatt atttgcggcg cttttttcac ttaccggagg ttatatggaa cctgatccca
    22981 cgcctctccc tcgacggaga ttaaaacttt tccggtaagc ccgtcttttc acggcgttac
    23041 cggatgcgta aggccgtgac gttttaacgt ccctgctcag ctttattacc ttcaggtaag
    23101 gcttcgccac gcctgaagac atttctgtac tgtttcagac agtgcggagg gactccttat
    23161 gtttaaagaa atttttaccc ggctcattcg ccatttacct tcccgtctgg ttcatcgtga
    23221 tccattgcct ggcgcgcagc agacagtgaa tacggtggtc ccgccgtcct taagtgcgca
    23281 ttgcctgaaa atggcggtga tgcccgaaga agaattgtgg aaaacgttcg acacccatcc
    23341 ggaagggtta aatcaggcgg aagtggaatc tgcccgcgaa caacatggtg aaaataaatt
    23401 acccgcacaa caaccgtcgc cgtggtgggt acatttatgg gtctgctatc gcaacccctt
    23461 taatatttta ctcaccattc tcggcgctat ttcttacgcc acggaagatt tatttgctgc
    23521 gggcgttatc gcgctaatgg tcgctatttc tacgttgctg aactttattc aggaagcacg
    23581 ttccactaaa gcggcagatg ccctgaaagc gatggtcagc aatactgcga cggtgctgcg
    23641 cgtaattaac gacaaaggcg aaaatggctg gctggagatc ccgatcgacc agctggtgcc
    23701 cggcgatatt ataaaactgg cggcgggaga tatgatcccg gcagatttac gtatcttgca
    23761 ggcgcgggat ctgttcgtcg ctcaggcgtc gttaaccggt gagtctctgc ccgtagaaaa
    23821 agccgctacc actcgccagc cggagcacag caatccgctg gagtgcgaca cgctgtgttt
    23881 tatgggcacc accgtggtga gcggcacggc acaagcaatg gtgattgcta caggtgccaa
    23941 tacctggttt ggtcaactgg cggggcgtgt tagtgagcag gaaagcgagc cgaatgcctt
    24001 tcagcaaggg atcagccgcg tcagtatgct gctgattcgc tttatgctgg tgatggcacc
    24061 ggtggtgctg ttaatcaatg gctacaccaa aggcgactgg tgggaagcgg cgctgtttgc
    24121 gctttcggtc gcggtaggcc taacgccgga aatgttgccg atgattgtta cctcgacgct
    24181 ggcgcgcggc gcagtaaagc tgtcgaaaca gaaagtgatc gtcaaacatc tggatgctat
    24241 tcagaacttt ggcgcaatgg atattctgtg cactgataaa accggcaccc tgacgcagga
    24301 taaaattgtg ctggagaatc ataccgatat ctccggtaaa accagcgaac gcgtgctgca
    24361 tagcgcgtgg ttgaacagtc attatcagac cggacttaaa aacctgctcg atacagcggt
    24421 gctcgaaggt acggatgaag agtcagcgcg ctcgctggcc agtcgttggc aaaaaatcga
    24481 tgagattccg tttgatttcg agcgtcgccg gatgtcggtg gtagtggcag aaaataccga
    24541 gcaccatcag ctggtttgca aaggcgcatt gcaggaaatc ctcaatgtgt gttcgcaggt
    24601 gcgtcacaat ggcgagattg tgccgctcga tgacatcatg ctgcgtaaga ttaagcgggt
    24661 tactgatacg ctgaatcgtc aggggctgcg cgtggttgcg gtggcgacga aatacctgcc
    24721 agcgcgtgaa ggagattacc agcgggcgga tgaatccgac ctgatccttg aaggatatat
    24781 tgcttttctt gatccgccaa aagagacaac cgctccggca ctgaaggcat taaaagcgag
    24841 tggaattacc gtaaaaatcc ttactggcga cagtgagtta gttgcagcga aagtgtgcca
    24901 tgaagtgggg ctggacgcgg gagaggtggt cattggtagt gatattgaaa cgctatctga
    24961 cgacgaactg gcaaatcttg cacagcgcac cacgctgttt gcccgcctga cgccgatgca
    25021 taaagaacgc attgtgacct tactgaagcg cgaagggcat gtggttggct ttatgggcga
    25081 tggtattaat gatgcgcccg ccttacgcgc ggcggatatc ggcatttctg tggacggcgc
    25141 ggtagatatt gcccgtgaag cggctgatat catcctgctg gaaaaaagcc tgatggtgct
    25201 ggaagagggg gttattgagg gacgtcgcac tttcgccaac atgctgaaat acatcaaaat
    25261 gacggcgagt tctaacttcg gtaatgtgtt cagcgtgctg gtggcgagtg ctttcttgcc
    25321 cttcctgccg atgttgccgt tacacttgct tattcagaac ctgctgtacg atgtgtcaca
    25381 ggtggcgatc ccgtttgata atgtcgacga cgagcaaatt caaaagccgc agcgttggaa
    25441 tccggcggat ctggggcgct ttatgatctt cttcggaccg atcagctcga tcttcgatat
    25501 tctgacgttt tgcctgatgt ggtgggtatt ccatgccaac acgccggaaa cgcaaacgct
    25561 gttccagtcg ggatggtttg tggtgggctt actgtcgcaa acgctgattg tgcatatgat
    25621 ccgcacccgc cgtgtgccgt ttattcagag ctgtgcatcg tggccgttaa tgatcatgac
    25681 cgtgatcgtg atgattgtcg gtatcgcatt gccgttttca ccgctggcca gttatctgca
    25741 gctgcaggcg ctgccgttaa gctatttccc gtggctggtt gcgattctgg cagggtatat
    25801 gacattaacc cagttggtga aagggttcta tagccgtcgt tacggctggc aataaagaat
    25861 caactgggca tggtgacccg gattcagatt gcagacaaag tgagttttgt tcatgccgga
    25921 tgcggcgtaa atgccttatc cggccaacaa attcaataaa ttgcaggaat cacgtaggcc
    25981 tgataagcgt agcgcatcag gcgattttgc gtttgtcatc agacttaatc cgggcatgat
    26041 agcccggatt tccatcaaga ttagcgacga acagcgatcg cttcgatctc aatcttcacg
    26101 tctttcggca gacgggctac ttcaacgcaa gaacgtgccg ggaaggtggc gttgtgttcg
    26161 gtgaagaagg cttcgtaagt ggcgtttacg gttgcgaagt cgttcagatc ttttacaaac
    26221 acggtagttt taacgatgtc gcccactttc aggccagcgg cttcgacgat cgcttttacg
    26281 ttatccagcg actgacgtgc ctgtgcagcg acgtctgccg gtacttcgcc cgttttcgga
    26341 tttaccggga tctgaccgga ggtgatgatc atattgccca gatcaacgcc ctgtacgtaa
    26401 ggaccgatag ctgccggtgc attttccgtc gcgatagttt tgctcatgat ttctccttta
    26461 ttacagcggt aaaaagtctg gctcattata gggagccaga cttttattac caaccgcaat
    26521 taattggcca gcaccacatt atgggaaaac tctttttcac agtatttgca tttgagcgcg
    26581 atatcattgg cgcgttttcg cacggcaaag ctggaagaaa ccggttcggc atggctgata
    26641 cagttgctgt tcgggcagac cagcacattg tcgatgcgct ccggcagact tgggcgcgat
    26701 ttacccacca cttcatagtt gtcgatacgg ttaaccgtgg cttgcggcgc atacaatgcc
    26761 agttgatcta cttgatcttc actcaaaaag gtattttcga ttttgattag atctttgcgg
    26821 cccatctcgc cagaaggcag gttcagaccg atggtaatgc gctgatccgt ttcggtcagc
    26881 ttgaacagac tcaacagctt aaaaccgatc tgggcgggga tatggtcaat taccgtgccg
    26941 cgtttaatag cttcaacctg caatttatta tcgtgtgtca tctctatttc cccttacagt
    27001 accagatcgc gattcagaac cagtgccagt aacgcctggc gagcgaaaat cccattgcct
    27061 gcctgctgga agtaccaggc gtgtggcgtt ttatcaacat ccgtcgcaat ctcatcaaca
    27121 cgcggcagcg gatgcagcac tttcatattg gctttggcgt tgtggagatc gctggcgcga
    27181 agaacaaact gcgctttcac gttggcgtac tcggacgggt ccagacgctc tttttgcacg
    27241 cgggtcatgt acaggatgtc aacttccgcc atcacttctt caatagagct gtgcagactc
    27301 catgcgatcc ctttttcatc gagcatatcc agaatgtatt gcggcattgc cagcgcgtcc
    27361 ggcgcgatga agtaaaaacg gttgccgtcg aacttcgcta acgcctgagt cagggagtga
    27421 acggtgcggc catatttcag gtcaccaacc attgcgacgt ggagattgtc cagacgcccc
    27481 tgggtttcct gaatagtgaa taagtccagc aaggtttgcg tcggatgttg gttggagcca
    27541 tcaccggcat tcagtaccgg tacattgccg gaaaactcgg tggccaggcg cgccgcacct
    27601 tcctgcggat gacgcatcac tatcgcatcg acgtaagtgc tgataaccga aatggtatcg
    27661 gccagcgttt cgcccttttt acccagtgat gtattggcgc tgtcggagaa gcccaccacg
    27721 ctggcaccca ggcggtgcat ggaagtttcg aaagagaggc gggtacgggt agaggcttcg
    27781 aagaagcagc tggcaatgac tttgtgtttc aacagctctg gttgcgggtt tgctttcagt
    27841 tttgccgctg ttgccagcac cagattaagg tcatcgcgac taaggtcgtt tatggaaatg
    27901 atatgtttct gatatagcgg attagccatc ttttatctcc tgacgcctgg gcaaaaaaaa
    27961 gcccctcgat tgaggggctg ggaatgggtg atcaacggga agaaaaacgg caggccagcg
    28021 tcttttttca gacgcggtaa gacaaaatgt cgaacacact gaaccataca tcctcccggc
    28081 aaattgtccg gcattatact aatcgtcaga agcggtgcaa gcatttgatg caatattttg
    28141 tcagcgcaaa cggtttattt gaatttaaag tcaaaatata tgcattttta tttactgtga
    28201 ttctggaggg gaactgtcgt tcggtatcaa taattgcaga catttcctgc ttttttcttt
    28261 ttcactttca cgcaatcaga tatgcatttt attccattca tccgacttat agggcggagt
    28321 gtgaaaacga acggctaaca ctattgctta cagctcaggg atgcgcgcta tcactttaat
    28381 ttcaaaatca aagcctgcca gccatgtaac acccaccgcc gtccagtttg gataaggtgg
    28441 ggcgctaaat atttcatttt tcaccgtcat gatgtcttcg aattgttttt ctggatcggt
    28501 atgaaagctc gtaatatcaa tgatattgtc aaaagtgcat cccgcagcag ccagggtcgc
    28561 atgcaaatta tcaaatgcca gtctgacttg ttgctgaaaa tcgggttctg gtgatccgtc
    28621 ctctcgactt cctacttgcc cggaaacaaa cagcaaatcg ccggaacgaa tagccgcaga
    28681 ataacgatgt tctgcataca gtgaatgtcg gccagcaggg aaaacagcgg ttctttctac
    28741 catttggtta tcctcaagat ttacgacatg aacagaagat ttatatttac cgggagccgc
    28801 ttttagcgga cgacgtgagt aaacaaaacc cagacatcat ggataatggc tgggcttaat
    28861 tgagcgtagt cggttatgcg ccaaacgcgc catcaatggt atgcatcgcg ccggtaacaa
    28921 aactggcttc tggccctgct aaccatgcga ccataccagc gacctcttcc ggttgcccat
    28981 gtcttttgat ggccataaaa ccatgcaaca tatcgcgcat tggcccgttg gcgggattag
    29041 cgtcggtatc aattggccct ggctggacga cgttgatggt gatcccacgc ggtccaaaat
    29101 cacgggccag cccgcgcgcc atgccttgta gggcagattt actggcggca taagcagcca
    29161 tgcctgcaac aggcatacga tcgccattca cggagccgat gattaagatg cgcccgcctt
    29221 cgggcatctg ccgggcggct tcaacagagg catggtaagg agcatgaata ttgattttga
    29281 aaaggtgatc aatatcgtcg gcatttaatt ccagggcatc gccaaagacg ccaatacctg
    29341 catttaccac caggatatcc aatgcgccgc tcttacgaac gacatcaata acagcgtctc
    29401 tgtcagcact atctgtgaat actgctgtcg ctccagtctc ttgtgccagg cgttcagcgg
    29461 catctttcga ccccgcatag gtgaatcgta cattggcccc atcggtgacg aaacgacgta
    29521 cgatagcggc accgattcca cggctaccac cgaggatgag aactgtctta cctgtaaaag
    29581 cgcccataag gactccttga tttattatgt aacatgcatt acaaaactgt tttaactttc
    29641 tgtcaacaag ttttgtaatg gtcactaaaa aacaatctcg cgttccaggt cgtcccagac
    29701 gtttcgctcc tgagcaggca gtctctgcgg caaaagtgct ttttcaccaa aaaggtttcg
    29761 atgctgtcag tgttgctgaa gttactgatt atcttggtat taaccccccg agcctctacg
    29821 cggcttttgg cagtaaggct gggttattta gccgtgtact caatgaatat gtcggtacgg
    29881 aagctattcc gcttgtcgat attcttcgtg atgatcgtcc ggtaggggag tgcctggctg
    29941 aggtattaaa agaagcggcg cgcagatata gccaaaacgg cggctgcgct ggctgtatgg
    30001 ttcttgaagg cattcatagt catgatccac aagcgcgtga tattgccgtt caatattatc
    30061 acgccgcaga aacggccatt tatgactata tcgccaggcg gcatccacaa agcgcacaat
    30121 gtgtgactga ttttatgagt accgtgatgt cagggctttc tgcgaaggca cgagaggggc
    30181 actccctcga acaactctgt gcaacagctg cactggcggg gggagcgata aaaactattc
    30241 tcaaggagtg atgcgggcct tgacccgaaa ggcgggaacg cgcctgccga taagttgtga
    30301 taagacaata attcacgcgt taaggctagc ggaattgatc atcttttcgt ataacgctag
    30361 aaatgaaacg ttgttttaat taaggagtgg aaaagatgat catcggaaat attcataatc
    30421 ttcagccgtg gctaccccag gagttacgcc aggcgattga gcatatcaaa gcacacgtta
    30481 cggtagaaac gccaaagggc aagcacgata tcgaaggcaa tcgtctgttt tatcttatct
    30541 cggaagatat gaccgagccg tacgaagctc gccgtgcgga gtaccatgcc cgctatctcg
    30601 acattcagat tgtgttaaaa ggtcaggaag gcatgacctt cagcacacaa ccggcaggta
    30661 cgccggatac cgactggtta gctgataaag acatcgcatt tttgccggaa ggcgttgatg
    30721 agaaaacagt tatccttaat gaaggtgatt ttgttgtgtt ttatccgggg gaagtgcata
    30781 aaccgctgtg cgcagtgggc gcgccagccc aggttcgcaa agcagtagtg aagatgctga
    30841 tggcgtgatg acttttcgcc gtaaataact ccaggtttac ggcgagtttg tgaaaagagc
    30901 gttttttgat atttttttgt gagtaaaatt tgtaatgctt agacgttctt attaactcaa
    30961 ggagttcgtc atgagcaaaa tatcagattt gaattattct caacacatta catcagccga
    31021 taattttaaa ccaaaaaatg aagtcttaaa tacctggtgt gtgggaatga atgattttgc
    31081 ccgtattgcc ggggggcagg atagcagaag gaatattctt tctcctggag catttttgga
    31141 gtttttggca aaaatattta ccctgggtta tgtggatttt agcaaacgct ccaacgaagc
    31201 gggtagaaat atgatggctc atattgagtc ctcatcttat atcaaaaata atgatggcag
    31261 tgaaataatg aagtttgtta tgaataatcc tgaaggtgag cgagcggatt catctaaggt
    31321 gattatagaa atttcacttt ccactactac tatggggact cgtcaaggac atacagccat
    31381 tatatttcca caatctgatt ttttgagttt tcgttatgaa gggaagtctt ttgaaagaaa
    31441 agatgatagt tcattacacc tgattactaa caaggttctg g
//
LOCUS       DAZKFU020000027        31348 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZKFU020000027 DAZKFU020000000
VERSION     DAZKFU020000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.027"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..556
                     /locus_tag="ACHOQQ_RS05055"
                     /old_locus_tag="pgaptmp_001011"
     CDS             <1..556
                     /locus_tag="ACHOQQ_RS05055"
                     /old_locus_tag="pgaptmp_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGU4469295.1"
                     /translation="WSGRLYPDSSAFEHNWPTTNEFVENSTLAMPESLPEELTVDYWF
                     RKNKVSEHVNAKHVFTARAGLVRFKAVAEGDDSKKSYKRLHRWRYSPTAAYGNNEVHL
                     HPYKPRRITVAEALAIQSLPKEFELPSYMTLSNMFKTVGNGVPYLAALGIAKSILEHL
                     GIKDEFNSNESCECDSKSTQKQAV"
     gene            492..1568
                     /locus_tag="ACHOQQ_RS05060"
                     /old_locus_tag="pgaptmp_001012"
     CDS             492..1568
                     /locus_tag="ACHOQQ_RS05060"
                     /old_locus_tag="pgaptmp_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4469296.1"
                     /translation="MNLTAMNLVNAIANLPKNRQFDYVNESNAGKIVIDSIKLPEGPI
                     RFKRFNPTKGETLRDAKVDSISSQMLWRLANAIQEDAPINLDRVFGASYNSRSVIESL
                     LAHTPQFYWCKLDRLEVINTKQTIKKGHKHLIYLPNLPHENGKLSEYKTNIVISEMNT
                     EVVYQSVDSETIRPIEGMSIEESRRHAQIQIALVKIGHHLGYRTWVAANDRGLKYNGK
                     AIAQMDGVIDSLSNEKVLSSYSEAIAGARLIDCIWFRNGKLMPAVMEIEHSTGIIRGL
                     ARMKNFCDYAPRLQDIRWTIVAPDEYREKVIEFANRPQFRELDTKFFPYSAVEELYSL
                     CERRKPQGITDQFLDSFMEKCIKH"
     gene            2278..2467
                     /locus_tag="ACHOQQ_RS05065"
                     /old_locus_tag="pgaptmp_001013"
                     /pseudo
     CDS             2278..2467
                     /locus_tag="ACHOQQ_RS05065"
                     /old_locus_tag="pgaptmp_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999882.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2950..3342
                     /locus_tag="ACHOQQ_RS05070"
                     /old_locus_tag="pgaptmp_001014"
     CDS             2950..3342
                     /locus_tag="ACHOQQ_RS05070"
                     /old_locus_tag="pgaptmp_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4469297.1"
                     /translation="MRDIQQVLERWGAWAANNHENVTWSSVAAGFKGMIPPKITSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGKTFMALARKHGCSDGHIGKRLQKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(4027..4401)
                     /locus_tag="ACHOQQ_RS05075"
                     /old_locus_tag="pgaptmp_001015"
     CDS             complement(4027..4401)
                     /locus_tag="ACHOQQ_RS05075"
                     /old_locus_tag="pgaptmp_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469298.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDAAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            5124..5948
                     /gene="dinD"
                     /locus_tag="ACHOQQ_RS05080"
                     /old_locus_tag="pgaptmp_001016"
     CDS             5124..5948
                     /gene="dinD"
                     /locus_tag="ACHOQQ_RS05080"
                     /old_locus_tag="pgaptmp_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGU4469299.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQISIKQLENCVKITEKK"
     gene            6240..6857
                     /gene="yicG"
                     /locus_tag="ACHOQQ_RS05085"
                     /old_locus_tag="pgaptmp_001017"
     CDS             6240..6857
                     /gene="yicG"
                     /locus_tag="ACHOQQ_RS05085"
                     /old_locus_tag="pgaptmp_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGU4469300.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(6854..8536)
                     /gene="ligB"
                     /locus_tag="ACHOQQ_RS05090"
                     /old_locus_tag="pgaptmp_001018"
     CDS             complement(6854..8536)
                     /gene="ligB"
                     /locus_tag="ACHOQQ_RS05090"
                     /old_locus_tag="pgaptmp_001018"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGU4469301.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVADVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNQARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLVSTEQFWQQLPGIGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            8794..9417
                     /gene="gmk"
                     /locus_tag="ACHOQQ_RS05095"
                     /old_locus_tag="pgaptmp_001019"
     CDS             8794..9417
                     /gene="gmk"
                     /locus_tag="ACHOQQ_RS05095"
                     /old_locus_tag="pgaptmp_001019"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGU4469302.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9472..9747
                     /gene="rpoZ"
                     /locus_tag="ACHOQQ_RS05100"
                     /old_locus_tag="pgaptmp_001020"
     CDS             9472..9747
                     /gene="rpoZ"
                     /locus_tag="ACHOQQ_RS05100"
                     /old_locus_tag="pgaptmp_001020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGU4469303.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            9766..11874
                     /gene="spoT"
                     /locus_tag="ACHOQQ_RS05105"
                     /old_locus_tag="pgaptmp_001021"
     CDS             9766..11874
                     /gene="spoT"
                     /locus_tag="ACHOQQ_RS05105"
                     /old_locus_tag="pgaptmp_001021"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGU4469304.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            11881..12570
                     /gene="trmH"
                     /locus_tag="ACHOQQ_RS05110"
                     /old_locus_tag="pgaptmp_001022"
     CDS             11881..12570
                     /gene="trmH"
                     /locus_tag="ACHOQQ_RS05110"
                     /old_locus_tag="pgaptmp_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGU4469305.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITEEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12576..14657
                     /gene="recG"
                     /locus_tag="ACHOQQ_RS05115"
                     /old_locus_tag="pgaptmp_001023"
     CDS             12576..14657
                     /gene="recG"
                     /locus_tag="ACHOQQ_RS05115"
                     /old_locus_tag="pgaptmp_001023"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGU4469306.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLQTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALNVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(14833..16038)
                     /gene="gltS"
                     /locus_tag="ACHOQQ_RS05120"
                     /old_locus_tag="pgaptmp_001024"
     CDS             complement(14833..16038)
                     /gene="gltS"
                     /locus_tag="ACHOQQ_RS05120"
                     /old_locus_tag="pgaptmp_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGU4469307.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16317..17708
                     /gene="xanP"
                     /locus_tag="ACHOQQ_RS05125"
                     /old_locus_tag="pgaptmp_001025"
     CDS             16317..17708
                     /gene="xanP"
                     /locus_tag="ACHOQQ_RS05125"
                     /old_locus_tag="pgaptmp_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGU4469308.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            17829..19538
                     /gene="yicH"
                     /locus_tag="ACHOQQ_RS05130"
                     /old_locus_tag="pgaptmp_001026"
     CDS             17829..19538
                     /gene="yicH"
                     /locus_tag="ACHOQQ_RS05130"
                     /old_locus_tag="pgaptmp_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4469309.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLLLDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PAQ"
     gene            complement(19582..20262)
                     /locus_tag="ACHOQQ_RS05135"
                     /old_locus_tag="pgaptmp_001027"
     CDS             complement(19582..20262)
                     /locus_tag="ACHOQQ_RS05135"
                     /old_locus_tag="pgaptmp_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HGU4469310.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEASLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(20243..21175)
                     /locus_tag="ACHOQQ_RS05140"
                     /old_locus_tag="pgaptmp_001028"
     CDS             complement(20243..21175)
                     /locus_tag="ACHOQQ_RS05140"
                     /old_locus_tag="pgaptmp_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGU4469311.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(21223..22083)
                     /locus_tag="ACHOQQ_RS05145"
                     /old_locus_tag="pgaptmp_001029"
     CDS             complement(21223..22083)
                     /locus_tag="ACHOQQ_RS05145"
                     /old_locus_tag="pgaptmp_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HGU4469312.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMNILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            complement(22164..23015)
                     /locus_tag="ACHOQQ_RS05150"
                     /old_locus_tag="pgaptmp_001030"
     CDS             complement(22164..23015)
                     /locus_tag="ACHOQQ_RS05150"
                     /old_locus_tag="pgaptmp_001030"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGU4469313.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAYAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVTLALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADMMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            complement(23027..24118)
                     /locus_tag="ACHOQQ_RS05155"
                     /old_locus_tag="pgaptmp_001031"
     CDS             complement(23027..24118)
                     /locus_tag="ACHOQQ_RS05155"
                     /old_locus_tag="pgaptmp_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGU4469314.1"
                     /translation="MKDNKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            complement(24143..24457)
                     /locus_tag="ACHOQQ_RS05160"
                     /old_locus_tag="pgaptmp_001032"
     CDS             complement(24143..24457)
                     /locus_tag="ACHOQQ_RS05160"
                     /old_locus_tag="pgaptmp_001032"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGU4469315.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(24475..24945)
                     /locus_tag="ACHOQQ_RS05165"
                     /old_locus_tag="pgaptmp_001033"
     CDS             complement(24475..24945)
                     /locus_tag="ACHOQQ_RS05165"
                     /old_locus_tag="pgaptmp_001033"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HGU4469316.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(24972..26525)
                     /locus_tag="ACHOQQ_RS05170"
                     /old_locus_tag="pgaptmp_001034"
     CDS             complement(24972..26525)
                     /locus_tag="ACHOQQ_RS05170"
                     /old_locus_tag="pgaptmp_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGU4469317.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVKIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLGAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(26820..29138)
                     /gene="yicI"
                     /locus_tag="ACHOQQ_RS05175"
                     /old_locus_tag="pgaptmp_001035"
     CDS             complement(26820..29138)
                     /gene="yicI"
                     /locus_tag="ACHOQQ_RS05175"
                     /old_locus_tag="pgaptmp_001035"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGU4469318.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKETVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANAQGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAW
                     HEGTAFQLFNLQDGHEAVCEVPAADGSVIFTLKATRTGNTITVTGAGEVRNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(29148..30530)
                     /gene="yicJ"
                     /locus_tag="ACHOQQ_RS05180"
                     /old_locus_tag="pgaptmp_001036"
     CDS             complement(29148..30530)
                     /gene="yicJ"
                     /locus_tag="ACHOQQ_RS05180"
                     /old_locus_tag="pgaptmp_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGU4469319.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISIAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIVAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            30823..30917
                     /locus_tag="ACHOQQ_RS05185"
                     /old_locus_tag="pgaptmp_001037"
     tRNA            30823..30917
                     /locus_tag="ACHOQQ_RS05185"
                     /old_locus_tag="pgaptmp_001037"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30857..30859,aa:Sec,seq:tca)
     gene            30996..31157
                     /gene="rhuM"
                     /locus_tag="ACHOQQ_RS05190"
                     /old_locus_tag="pgaptmp_001038"
                     /pseudo
     CDS             30996..31157
                     /gene="rhuM"
                     /locus_tag="ACHOQQ_RS05190"
                     /old_locus_tag="pgaptmp_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT         8209 a         8139 c         7538 g         7462 t
ORIGIN      
        1 ctggagtgga agattatacc cagatagtag tgcttttgaa cataattggc caactacgaa
       61 tgaatttgtt gagaactcaa cactggcaat gcctgagtca cttcctgaag agttaactgt
      121 tgactactgg tttaggaaaa acaaagtttc tgaacatgtg aacgcaaaac atgtgtttac
      181 tgctagggct gggttagttc gctttaaagc tgtagctgaa ggggacgata gcaaaaaatc
      241 ttacaagcgt cttcatcgct ggagatattc tccaacagct gcttacggta ataatgaagt
      301 acatttgcat ccatataagc cacgtagaat aacagttgca gaagcacttg ccattcagtc
      361 tttaccgaaa gaattcgaat tgccatctta catgactctt tcaaatatgt ttaaaactgt
      421 tggcaatggt gttccttacc ttgcggcttt aggcatagca aaatccatat tagagcattt
      481 agggataaaa gatgaattta acagcaatga atcttgtgaa tgcgatagca aatctaccca
      541 aaaacaggca gtttgattat gtgaatgaaa gtaatgccgg gaagattgtt atcgactcga
      601 ttaagttacc agaaggccca atacgcttta aacgtttcaa tccaaccaaa ggtgaaacgc
      661 tcagagatgc caaagttgat agcatatcta gccaaatgct atggcgtttg gctaacgcaa
      721 ttcaagagga tgcgcctatc aatttagata gagtattcgg tgcaagctat aactctcgtt
      781 cagttattga aagtttgctt gctcatacac ctcaatttta ttggtgtaaa ctcgaccgct
      841 tagaggttat taatacgaaa cagacgatta aaaaaggcca caagcatctt atatacttac
      901 cgaatttacc acatgaaaac ggcaagcttt ctgaatacaa aactaatatt gtaatatcag
      961 agatgaacac agaagttgta taccaaagtg ttgattcaga aacaatccgt ccaattgaag
     1021 gcatgtctat tgaagaatca agaagacatg cacaaatcca gatagcatta gtaaaaatcg
     1081 gtcatcatct gggatacaga acatgggttg cagctaatga ccgaggatta aaatacaatg
     1141 gtaaagcaat cgctcaaatg gatggtgtca ttgacagtct gtctaatgaa aaagtcctaa
     1201 gttcctacag cgaagccatt gctggcgcaa gattgataga ttgcatctgg tttagaaatg
     1261 gtaaattgat gcctgccgtg atggagatcg agcatagtac cggaataata aggggtttag
     1321 cacgtatgaa gaacttctgt gattatgctc cccgtctgca agatatacga tggacaatcg
     1381 tagctccaga tgaatataga gaaaaagtaa ttgagtttgc aaatagacca caatttcgtg
     1441 agctagacac aaaatttttt ccttactctg ccgtagaaga attgtattct ctttgtgagc
     1501 gccgaaagcc tcaaggaata accgatcaat tcttagattc tttcatggaa aaatgcatca
     1561 aacattaaaa aagccgggat aaacccggtt ctgatgacta aatagctgtt cggaatagta
     1621 gatcacttcg tatttgaggg aactcaatcc agattatgcg atctgatcaa taacacaaat
     1681 cccaaataag caaatcggac tgagcgatgt cgatacgttt aacgatctaa ttaaggccgt
     1741 aaaactattg acgacatggg cgaaagataa cttgcgagca atatacctca tgcacatcaa
     1801 agaaatgtca caacggaagg cctgatttta aaatcgcgta agaaatctgc aattacccgt
     1861 agtgattgcg gctgacagta agtcagttac aaagtcagtt acatatcgct cctgatgcac
     1921 acattccgta ggacaacaat tccccgaaaa gtgcgcagaa aactgcgcaa attcgatcat
     1981 aaaaccgatc acaaaatcga ttatgctgac caccagcaca aagccagtaa cgacgcggct
     2041 tgcatgggga aaaccaccgg aaaaagtgcg caaaacgaaa cacaacagcc gcgcaaaacg
     2101 aaaccgatac gtgatgatcg atttttacgc cagaactgac cagagcaagg ggcagatttc
     2161 cgcccttgta ttttgtgaat gactaaaagt cacccgcaaa aatgaacgtt tttacagggc
     2221 aggaaacgag agtactggtt aacattcctg gcataaccgc aactcacagg gggaccaatg
     2281 accgaccgtt caccaacaga ggtaattcac gggattattg aagagcagga ggagcgcgag
     2341 ttaatcctca tgattgaaga gcagagagaa cgccagcgta aacaggagac cacgcagccg
     2401 gacagcgtga tcgcagaaaa ttatgcgcgt gttgtggctg atgtgccgtg atggtgtcga
     2461 actgagtatc tttaagcgac ttcgtgactg gttttaaacg agtcgcaaaa aaagccggag
     2521 aaaaaccacg aatctggggt tttttctaag cacgctgtac acggtggaac atatggtggt
     2581 caggagacgg caggagcaac gccagaacgc gaccccgtac cagtggcata agccggagcg
     2641 gttacggcgg taacttgttg attaaaaggc ggctcaattc tgagccgggt agctgacggc
     2701 gttaactggc tgatattgtt acaaagctca tttttgagct ttggcctaac ctactgatat
     2761 tttcggaaac ctcaatttga ggagaccgca aacgcaaaat tgcgctggct acatccctcg
     2821 cttctgagga ctgttaacaa gctgcctgaa agacagtcac aagctacgaa attttcgtag
     2881 gttaacttct atccagtaca tacccagaat tacgggtatg taaccacagc cattacgagg
     2941 tgattgttga tgcgtgatat tcagcaggtt ctggaacgat ggggggcatg ggcggcaaat
     3001 aatcacgaga atgtgacctg gtcatccgtt gctgccggat ttaaggggat gattccgcca
     3061 aagataacat ctcgtccaca gtgctgcgac gatgacgcga tggtgatatg cgggtgcata
     3121 gcccgccttt accggaacaa tcgcgatctg catgacttgc tggttgatta ctacgtgctg
     3181 gggaaaacgt ttatggcgct ggcacgtaag catgggtgct ctgatggaca tatcggaaag
     3241 cggttacaga aagcggaagg tgttgttgaa ggcatgctga tgatgctgga tgtgaagctg
     3301 gagatggatc ggtatgttga gcgcgattca gtgacggcat aacgcgcaga aaaaaccgca
     3361 gctatgagaa ctgcggtgga gaagtaagac ttgccagtca atcatgaaaa acgtaatcgc
     3421 ctgataatta tgccacgaga caaagggcgg tgcaccctga atggtgataa tcaccgcgca
     3481 gaccatccag acaaccataa cgaacatcgt tacagttgga gcttctacca ccattggtgg
     3541 aaaaggtgca catctgaatc atcattacgg cgatcatgcc ttccctaccg gaacaaggaa
     3601 ggctggccta cgaaaatttc gtaaccctcc cccgcacaat gtattaaata atcactacaa
     3661 gccattgatt aaacttaatt atcgcgaatc gcgagaatta ggtttttcag tctggtaaac
     3721 agttaactgg aaaaaaagcc aggtagggtt ccagatgctc ccccatcgat ataaccccca
     3781 tctctccctg accacggttg cccgttaagt gcaagcgcac aggaacgacc tcgctaacca
     3841 gttatcagcg ctcacgctca aatatccaca gcaatgggga taaatcgcat tctgccgcag
     3901 tattcgctat cagggcaact ccgcaggaaa tgatatacag gctgtcagaa tgcttaaaat
     3961 tagatggacg gatgacaggg caggatggtg agacctacac aaaaagcgca ggcctccatg
     4021 actcggtcag gacagtttgt gtttaaccag aatatttagc gcctgtatcg gggcttcgtg
     4081 gagttcccgc agacattcag ggtcagttac atgttcctgg tatcttgttt tctcaagtaa
     4141 caagcggtgc atatgcagca tacgggcaag agccgcgcac acttcctcaa atgcggcatc
     4201 ccgcttaccg tcactgtcag agccacaaaa cagcggtaca acctccacgc cgtcgctgtg
     4261 ggcctttagc caggcatcaa ccacggttaa atcatcggtg aagaaccacg cactgttagc
     4321 gctgaatcgc attttccagg cttccggtac tggttgcggg gagtatgaat atgctggtgg
     4381 tatcttgtcg tgagtatcca taggctatcg ttccgtcatt ctgttattca ccacggcgct
     4441 aaaaacgcca tgaaggacca gaataccgat tgcctcgttt gctgtatgag gtgcttacca
     4501 cacattgggt agacggatgt atcaggcgga agtgtaatta accggaaaaa atccctttca
     4561 gtctgacggg caaaaaaacc gtagtgcgat aacactacgg catgccacca aagaaacaca
     4621 acaagaggtc aacaatgagt aatggatatg accagacgat tttatcatgc cggaacaaaa
     4681 gttatgtgac acagtcggat aaaaacaggt aaagcccacc agcttcataa cggataaccg
     4741 gaaaaaatac gctgagtatt tgccctaata gtcaccatat cggcgctaca cctgaaaaaa
     4801 tcgtgtacac aagggtgtac acaatcaaaa tcttcattac agaaacccaa gcaatgaagc
     4861 aactttgatg cagatactca atgaacagat tcaattgaat aactcgtaac gccaattctt
     4921 acttttcagc cttcacaaat gccgcccact caaacagagc ggcatttttc ttctccgcaa
     4981 ctttcaattc tgttttgcgt gcctgttcca gattttgcga tgtctttttg cccagcacac
     5041 tgagaacgtg agatactcac aactgtatat aaatacagtt acagatttac tttctttgca
     5101 atcgatatca catggagtgg gcaatgaacg aacatcatca accttttgaa gaaatacggc
     5161 attatggcac tgaagggcaa gaattttggt cagcgagaga attggcaccg ttactggatt
     5221 atcgggactg gcgcaatttt caaaaggtac tggcccgcgc gactcaagcg tgcgaagcca
     5281 gcaatcaagc tgcatctgac catttcgttg aaaccaccaa aatggttgta ttaggttcag
     5341 gggctcaacg agaacttgaa gatgttcatc tctcccgcta tgcctgttac ctggtagtac
     5401 aaaacggcga ccctgcgaaa ccggttattg cggcagggca aacttatttt gctatccaga
     5461 cccgacggca ggagctggct gacgatgaag ctttcaggca acttcgtgaa gacgaaaagc
     5521 gtctttttct gagaaatgaa ttgaaagaac ataacaaaca attagttgag gcagcgcagc
     5581 aagctggggt agcgacagct atcgatttcg ccatatttca gaatcatggt taccgggggc
     5641 tgtatggtgg attagatcag aaagctattc atcagcggaa ggggctgaaa aagagtcaga
     5701 agatcctgga tcatatgggt tcaacagaac tggcggctaa tctctttcga gctacccaaa
     5761 cagaagaaaa actcaagcgg gatggcgtga attcaaaaca gcaagctaac accacacact
     5821 ttgacgtggg tcgcaaggtg agacaaacca ttcaggaact tggcggaacc atgcctgaag
     5881 agttgccgac cccgcagatc agcatcaagc agttggaaaa ctgtgtaaaa attacagaga
     5941 agaaatagca gaaactaaaa actctttttg ttgattgaga cacccgatgc gtaagccaaa
     6001 gtccaggtgc ggtgaataca atagctaaac attgcacctt tcttttccac ccctcagttt
     6061 taacctattt tttcttatgc attttctcag acaagaagtc agaagaatgc atctccgcta
     6121 cagaaaatag cgatttcaca taactacaag ttatcaattc cccctcacct taaaaaatct
     6181 caatcgtgac aatgcgcaca aatcgctacc ctgcaagaca gatttttagg gagagaacca
     6241 tgctgttaca cattttgtat ttggttggca ttactgccga agccatgacg ggggcgctgg
     6301 cggccgggcg acggcgcatg gatacatttg gcgtaattat tattgcgacc gccaccgcaa
     6361 ttggcggagg gtcagtgcgc gatattctgc tgggccacta tccgcttggc tgggtcaaac
     6421 acccggaata tgtgattatc gtcgctaccg ccgcagtact caccactatc gttgctcctg
     6481 taatgcctta cctgcgcaaa gtgtttctgg tgctcgatgc gctcggactg gtggtctttt
     6541 ctatcattgg cgcacaggtt gcactggata tggggcacgg cccaattatt gccgttgtcg
     6601 cggcggtaac caccggcgtg tttggcggcg ttttgcgcga tatgttctgt aaacgcatcc
     6661 cactggtatt ccagaaagag ttgtatgccg gtgtctcatt tgcctccgcc gtgctgtaca
     6721 tcgcgctgca acactatgtt tctaaccatg atgtggtgat tatctccacc ctggtattcg
     6781 gcttttttgc ccgtttactg gcgctacgtc ttaagctggg attaccggtt ttttactaca
     6841 gccacgaagg tcactaaggt tcaaaacctg tgatctgctg ggcagccagc caactgccca
     6901 gcttcttgat ttgcgcattt tccttccatt caataacctg tctggcgcgc ccagatccaa
     6961 tccccggcag ctgctgccag aactgttccg tgctaactaa aagttgcgac caggaccgtt
     7021 catcactggc attaagcgcc gcccgggtta gcggtattcc cattgccatc acccagcgag
     7081 taaaaggctg cttacgagcc tgattaaact gatgccataa ctgcgcactt ttacttttcg
     7141 cgatccccgg cgtgttctgt aattgctctg gcgttaataa aagccaggaa aagatatgct
     7201 caaagcgatg agtctgatgc agcgcgcgcc aaccggcctc accaatgcca tccagcccca
     7261 gaacctgttt tgaccccagc cagactaagc gtgaaatgaa ctgttcctga caaacatcag
     7321 aagcaaagta gcaggtcaac gagttaaagc ggttttctgg tggtgtcggt tttgtacgtt
     7381 ctgcaccgcg ccagaccaca tcatcaatgc gaggaatacc ctgaccggca aggctgacga
     7441 ggatctgatc accaggtgca atatcccact cctgccagcg cctgacggaa ccaatattca
     7501 cccgctggac ttttttatca tccagcatga caggtgcgag tgacgcaacc accgatattt
     7561 taccgctctt acccaccgca aactgaatcg ccttcacatc ggcaacctga gctacaggtt
     7621 gatatttcca ggctaccagc cactctgcct ggcccggtag ccaatggcgg gattctggct
     7681 ctttcgccgc tcgtacaact acgccatcgg tgacgaaggg taatttcgct ttccaccact
     7741 cattgcgtac gcgcgcaact tcatcagcat ttttcaccgc acgggtatac gtctgcgtta
     7801 gagtaaaacc agcggtagcc agctctttta aacgatcgga cattaactgc ggtccatccg
     7861 gccatgccca gacaaaaaca gccagtgagt ttagcgtgtc gctattcccc tggcgcatca
     7921 tcaggccagc aacttttgcg cgggcattta ttccccccat ttgttgttgg atatgcccct
     7981 cgcgctggag aaatatttcc ccctgaagcg tactgttggc taaaggcccg ctaacggttt
     8041 gcggcacagc ggaaattaag ctaactttct gcgtccagtc ctcgcctttc aggccattac
     8101 cgcgactgat tgctttgttc agtttcccgt cccgataaac cagggttacc gcaacgccat
     8161 caacttttgg ctgcacccaa agatcgctac gttctcgcat ccacagactt aatgcgtttt
     8221 tatccaccat tttgcgtaca cccgtatggg caaccggatg cataaccgca ccatttaaag
     8281 gcggcatcat cacgtcgcgg ggctcgctcc caaagcagcg ttgccactgc gtaagacggg
     8341 cgcttaactg atcgtaaaca ccgtcttcca cctcactttt tccttccttc cagtagtcat
     8401 cgtcccactg ttttatttgc tgttgcaggc gggaaatttc ttcctgtgct ctggctggcg
     8461 accaggccgg acagaccgcc cacacagatg attgccagca caagatactt attaatatcg
     8521 ccatccatac tttcatcatc acctccgctg tagatagtca ggcagatata caacgtgatg
     8581 aaagcaaagc cgagtggcaa aaacggagtc tgcgaggacg cttcctgaga atcgtcttta
     8641 ttgcagtgaa tcactggcaa atgcggaagc agctacgcaa aacgcaacaa ctttgcgcaa
     8701 aaagtgtgag caagggctac gtcacatggc cgcgccgtgt ataataagct cgtatgtagg
     8761 ctttatttcg ctaatcacat acgaaagata ctcatggctc aaggcacgct ttatattgtt
     8821 tctgccccca gtggcgcggg taaatccagc ctgattcagg ctttattaaa aacccaaccg
     8881 ttgtatgaca cccaagtttc tgtttcacac accacacgcc aaccgcgtcc tggtgaagtc
     8941 cacggtgaac attatttctt tgttaatcat gatgaattta aagaaatgat tagcagagat
     9001 gcgttcctcg aacacgcaga agtttttggt aattactatg gcacttcgcg tgaggccatt
     9061 gagcaagtac tggcgaccgg tgtcgatgtt tttctcgata tcgactggca gggtgcgcag
     9121 caaattcgcc agaagatgcc gcacgcgcgg agtatcttta ttttaccgcc gtccaaaatt
     9181 gaactggacc gccgtctacg cggtcgcggt caggacagcg aagaggtcat tgcaaagcgt
     9241 atggcgcaag ctgttgcaga aatgagccat tacgccgaat atgattatct gattgtgaat
     9301 gatgacttcg ataccgcgtt gaccgatttg aagaccatta ttcgcgccga acgtctgcgc
     9361 atgagccgcc aaaagcagcg tcatgacgct ttaatcagca aattgttggc agactgaacc
     9421 tgatttcagt atcatgccca gtcatttctt cacctgtgga gctttttaag tatggcacgc
     9481 gtaactgttc aggacgctgt agagaaaatt ggtaaccgtt ttgacctggt actggtcgcc
     9541 gcgcgtcgcg ctcgtcagat gcaggtaggc ggaaaggatc cgctggtacc ggaagaaaac
     9601 gataaaacca ctgtaatcgc gctgcgcgaa atcgaagaag gtctgatcaa caaccagatc
     9661 ctcgacgttc gcgaacgcca ggaacagcaa gagcaggaag ccgctgaatt acaagccgtt
     9721 accgctattg ctgaaggtcg tcgttaatca caaagcgggt cgcccttgta tctgtttgaa
     9781 agcctgaatc agctgattca aaactacctg ccggaagacc aaatcaagcg tctgcggcag
     9841 gcgtatctcg ttgcacgtga tgctcacgag gggcaaacac gttcaagcgg tgaaccctat
     9901 atcacgcacc cggtagctgt tgcctgcatt ctggccgaga tgaaactcga ctatgaaacc
     9961 ctgatggcag cgctgctgca tgacgtgatt gaagatactc ccgccaccta ccaggatatg
    10021 gaacagcttt ttggtaaaag cgtcgccgag ctggtagagg gggtgtcgaa acttgataaa
    10081 ctcaagttcc gcgataagaa agaggcgcag gccgaaaact ttcgcaagat gattatggcg
    10141 atggtgcagg atatccgcgt cattctcatc aaacttgccg accgtaccca caacatgcgc
    10201 acgctgggct cacttcgccc ggacaaacgt cgccgcatcg cccgtgaaac tctcgaaatt
    10261 tatagccctc tggcgcaccg tttaggtatc caccacatta aaaccgaact cgaagagctg
    10321 ggttttgagg cgctgtatcc caatcgttac cgcgtaatta aagaagtggt gaaagccgcg
    10381 cgcggcaacc gtaaagagat gatccaaaaa atcctctctg aaatcgaagg gcgtttgcag
    10441 gaagcgggaa taccgtgccg cgtcagtggt cgcgagaagc atctttattc gatttactgc
    10501 aaaatggtgc tcaaagagca gcgttttcac tcgatcatgg acatctacgc tttccgcgtg
    10561 atcgtcaatg attccgacac ctgttatcgc gtgctcggcc agatgcatag cctgtataag
    10621 ccgcgtccgg gtcgcgtgaa agactacatc gctattccaa aagcgaacgg ctatcagtcg
    10681 ttgcacacct cgatgattgg cccgcacggc gtgccggttg aggtccaaat ccgtaccgaa
    10741 gatatggatc agatggcgga gatgggtgtt gccgcgcact gggcttataa agagcacggc
    10801 gaaaccagca cgactgcaca aatccgcgcc cagcgctgga tgcaaagcct gctggagctg
    10861 caacaaagcg ccggtagctc gtttgaattt atcgagagcg ttaaatccga tctcttcccg
    10921 gatgagattt acgttttcac accggaaggg cgcattgtcg aactgcctgc cggtgcaacg
    10981 cccgtcgact tcgcttatgc agtgcatacc gatatcggtc atgcctgcgt gggcgcacgc
    11041 gtcgaccgcc agccttaccc gctgtcgcag ccgcttacta gcggtcaaac cgttgaaatc
    11101 attaccgcac cgggcgctcg cccgaatgcc gcgtggctga actttgtcgt tagctcgaaa
    11161 gcgcgcgcca aaattcgtca gttgctgaaa aacctcaagc gtgatgattc tgtaagcctg
    11221 ggccgtcgtc tgctcaacca tgctttgggt ggtagccgta agctgaatga aatcccgcag
    11281 gaaaatattc agcgcgagct ggatcgtatg aagctggcaa cgcttgacga tctgctggca
    11341 gaaatcggac ttggtaacgc aatgagcgtg gtggtcgcga aaaatctgca acatggggac
    11401 gcctccattc caccggcaac ccaaagtcac ggacatctgc ccattaaagg tgccgatggc
    11461 gtgctgatca cctttgcgaa atgctgccgc cctattcctg gcgacccgat tatcgcccac
    11521 gtcagccccg gtaaaggtct ggtgatccac catgaatcct gccgtaacat ccgtggctac
    11581 cagaaagagc cagagaagtt tatggctgtg gaatgggata aagagacggc gcaggagttc
    11641 atcaccgaaa tcaaggtgga gatgttcaat catcagggcg cgctggcaaa cctgacggcg
    11701 gcaattaaca ccacgacttc gaatattcaa agtttgaata cggaagagaa agatggtcgc
    11761 gtctacagcg cctttattcg tctgaccgct cgtgaccgtg tgcatctggc gaatatcatg
    11821 cgcaaaatcc gcgtgatgcc agacgtgatt aaagtcaccc gaaaccgaaa ttaatgtttt
    11881 atgaacccaa cacgttatgc acgcatctgc gaaatgctcg ccaggcggca gcctgatctg
    11941 accgtctgca tggagcaggt ccacaaacct cataacgttt ctgcgattat tcgtaccgca
    12001 gatgccgttg gcgtacatga agttcacgcc gtctggcctg gtagccgcat gcgtaccatg
    12061 gcctctgcgg cggcgggtag caacagttgg gtgcaggtga aaacgcaccg caccattggc
    12121 gatgccgtcg ctcatctcaa aggccagggc atgcaaattc tggcaaccca tctttctgat
    12181 aacgctgtcg attttcgcga aattgattac actcgcccga cctgcatttt gatgggtcag
    12241 gagaaaacgg gcatcactga ggaagcgttg gccctggcag atcaggacat catcattccg
    12301 atgatcggca tggtgcagtc gctgaatgtt tccgttgcct cagccctcat tctttacgaa
    12361 gcccagcgtc agcggcaaaa tgcaggcatg tacctgcgtg aaaacagcat gttgccggaa
    12421 gcagagcaac aacgcctgtt gtttgaaggc ggctatccgg tgctggcgaa agtcgcaaaa
    12481 cgcaaaggcc tgccttaccc ccacgtcaat cagcaaggcg agatcgaagc tgatgccgac
    12541 tggtgggcta ccatgcaggc tgcagggtaa gtgccatgaa aggtcgcctg ttagatgctg
    12601 tcccgctcag ttccctaacg ggcgttggcg cagcgcttag taacaagctg gcgaaaatca
    12661 acctgcagac tgtgcaggat ctactcttac accttcccct gcgctacgaa gatcgcaccc
    12721 atctctaccc catcggagaa ctactaccgg gcgtttatgc cacggtggaa ggcgaagtgc
    12781 tgaactgcaa tatctccttc ggcggtcggc ggatgatgac ctgccagatc agcgacggtt
    12841 ccggcattct caccatgcgc tttttcaact tcaacgcggc aatgaaaaat agcctggcga
    12901 cgggccgccg cgtgttggcc tatggagaag caaagcgcgg taaatatggt gcggagatga
    12961 tccacccgga ataccgcgtg cagggcgatc tcagtacgcc agagttgcag gaaacgctca
    13021 cgccggttta tccaacaacc gaaggcgtaa agcaggccac gctgcgtaaa ttaaccgacc
    13081 aggcgctgga tctgctcgac acctgcgcca ttgaagaact cctgccgccg gaactgtcac
    13141 aaggaatgat gacgctaccg gaagcgttgc gcactttgca ccgcccgccg ccgacgctgc
    13201 aacttagcga tctggaaacc gggcagcatc cggcgcaacg tcgtctgatt ctggaagaac
    13261 tgctggcgca taacctcagc atgttagcct tacgcgccgg agcgcaacgt ttccatgccc
    13321 agccgctgag cgccaatgac acgctgaaaa ataaacttct tgccgcctta ccgttcaagc
    13381 caacgggcgc tcaggctcgc gtggtggcgg agatcgagcg cgatatggcg ctgaatgtgc
    13441 cgatgatgcg cctggtacag ggcgatgtag gttccggtaa aacgctggtc gctgccctcg
    13501 ccgcgctgcg tgcgattgcc cacggcaaac aggtggcatt gatggcacca accgaattac
    13561 tcgccgaaca gcacgccaat aacttccgca actggtttgc accgctcggt atcgaagtgg
    13621 gctggctcgc cggtaagcag aaaggtaaag cgcgactgtc tcagcaggaa gccatcgcca
    13681 gcggtcaggt gcagatgatt gttggtacgc acgccatctt ccaggaacag gtgcagttta
    13741 acggtctggc gctggtgatt atcgacgaac agcatcgttt tggcgtgcat cagcgtctgg
    13801 cattgtggga gaaaggccag cagcaaggct tccatccgca tcagttgatc atgaccgcca
    13861 cgccgatccc ccgcacgctg gcgatgactg cgtatgccga tctcgacacc tcggtcattg
    13921 atgaactgcc gccaggccgc acgccagtga ctacggtcgc tattcctgat actcgccgta
    13981 ccgacatcat tgaccgcgtg cgccacgcct gcatgactga aggtcgtcag gcgtactggg
    14041 tttgtacgtt gattgaagag tcggaattgc tggaagcgca ggcggcggaa gctacctggg
    14101 aagagttgaa actggcgcta ccagagttga atgttggcct ggtacacggg cggatgaaac
    14161 ctgccgagaa acaggcggtg atggcgtcgt ttaaacaagg tgagctacac ctgctggttg
    14221 ccacaaccgt tattgaagtc ggcgttgatg tgcctaacgc cagtctgatg attatcgaaa
    14281 acccggagcg tctgggtctg gcgcagttgc accagctacg tgggcgtgta ggtcgtggcg
    14341 cggtggcttc tcactgcgtg ctgctctata aaacgccgct ttctaaaacg gcgcaaattc
    14401 gcctgcaagt gctgcgcgac agcaacgacg ggtttgtgat tgcacaaaaa gatctggaga
    14461 ttcgcggccc tggcgaattg ttaggcactc gtcagacggg taatgctgaa tttaaagtgg
    14521 cggatttact gcgcgatcag gcgatgatcc cggaagttca gcgcctggca cgccatattc
    14581 acgaacgtta cccacaacag gcaaaagccc tgatagaacg ctggatgccg gagacggaac
    14641 gttactcgaa tgcgtaaaag gcggcagtga gaagaccgcc atttcaggtt accctacctt
    14701 cctgccggat gtgcttcgtc ctacaaaaac ttataaattc aataaattgt gaatcaatgc
    14761 gcagccttga taagcgaagc gtatcaggca tttgtctttg ttgcggcggc acttctaccg
    14821 ccgcttcatc ggttaacccg caaaaatcgg caacatcaaa tacagcttaa tcaccagcgc
    14881 attgacgata tcgataaaga acgcaccgac catcggcacc accagaaacg ccatgtgcga
    14941 cgggccaaaa cgttcagtga tcgcctgcat gttggcgatt gccgttggcg ttgcaccgag
    15001 gccaaaacca cagtggcccg cagccagcac tgccgcatcg tagtttttgc ccatcatgcg
    15061 ccaggtaacg aagatggcat acaacgccat gaagatggtc tgtaccacca gaatcgccag
    15121 catcggcagc gccagcgaag ccagctccca cagtttcagc cccatcagcg ccatcgccag
    15181 gaacaacgac aggcttacgt tacccagcac ggataccgca cgctcaaaga cgcggtaaaa
    15241 gcccatcatt gacagaccgt tgctcagaat cacgccaaca aacagtacac agacgaaggt
    15301 cggcagttca aaagcagtgc cagccaaaag ttgcgcaaca attttcccca ccgtcaggca
    15361 gatagcaatc agcgcgatag tttcaatcag caccagcgag gtgatcatgc ggcccacatc
    15421 cggcttttca aacgccgtcg ggacttcctg gtcatccgga ataccgttcg gcgtggtgga
    15481 gtgtttcacc agatagcgcg ccaccggacc gccaatcaag ccgcccagca ccagaccgaa
    15541 cgttgcacag gccatcgcca cttctgtcgc attggtgaag ccataacgtt caatgaacaa
    15601 tttactccat gcagctcccg taccgtgacc gccggaaagc gtaatagaac cggccaacag
    15661 ccccatcaac ggatcaagcc ctaacaggct agccatccca atgccaatgg cattttgcat
    15721 caccaacaga ccaacaacca caatcaggaa gatgccaacc acacgcccac cggcacgcaa
    15781 actggcaatg ttggcgttca ggccaatggt ggcgaagaat gccagcatta acggatcgcg
    15841 cagggacata tcaaagttga cttcccagcc catgcttttt ttcagtacca gtagcgccag
    15901 cgccaccaac aaaccacccg caacaggttc cggtatggtg tatttcttca aaaaggagac
    15961 ggaatggacc aacttacgcc cgagcagcaa cgtcagcgtt gcggcaacaa gcgttgctaa
    16021 agtatcgaga tgaaacatag ttactccttt gtatccgcat cactctttca tactcgtcat
    16081 acttcatcgc gcagtggcaa cttatgaact atgacgggta tcgagttatg ctttcctggg
    16141 tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa ataccaaaag
    16201 ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg gcaaacgttt
    16261 gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg ttttcgatgt
    16321 ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc gcagactcaa aacagcgaac
    16381 tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc gcctgtcagc
    16441 atttgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc caggcgctgg
    16501 gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc tccggtgtgg
    16561 catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg tctattcagg
    16621 gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg aaaaccggtg
    16681 gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg gcaagttgca
    16741 ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg ccgctggttt
    16801 ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg acctcaattg
    16861 gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat ctgctgctgg
    16921 caggcgtggt cttagcctta attatcctgc ttaaccgtca acgtaaccct tacttacgcg
    16981 tggcctcact ggtgattgcg atggcggccg gatatgcgct ggcgtggttt atgggcatgt
    17041 tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg ccgctctatt
    17101 acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt atgatcactt
    17161 cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag ccagtgtccg
    17221 gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg aactcgtttg
    17281 tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcagaacaac ggagtgatcc
    17341 agttgaccgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg ttgatcgtgc
    17401 tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg gttctgggcg
    17461 gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc gtttctcgtg
    17521 agccgctgaa ccgtcgggcg attctgatta tcgccctgtc gctggcggtt ggtctgggcg
    17581 tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac ctgctctcct
    17641 ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc ccaccagaaa
    17701 aacaataatt cactggcggc ggtaagataa ttaccgccgc agtctcacct ctttcaccat
    17761 tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat ttcacgggat
    17821 ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct ctgttagtgg
    17881 tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat atcagcgcat
    17941 gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac cgtttttccg
    18001 cgccatctca tattgtgctg gagaacgtca cgtttggtcg tgatggccag cccgcgaccc
    18061 tggtggccaa aagtgtcgac attgcgctaa gtagtcggca actgaccgaa ccacgccatg
    18121 tcgataccat cctgctggaa aacggaacgc tgaatctcac cgaccagacc gcgccgctac
    18181 cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg aatagcgaat
    18241 ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca gaagccggta
    18301 aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg ctcaatgatg
    18361 ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt acgctgacta
    18421 acctgggtgc cgacatcgcg cgcgggacat taaccggaaa cgcgcagcgt aacgccgacg
    18481 gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc gaaaaatcgc
    18541 taaccgactt ctttgcacca ttacgctctg tcccgtcgtt gcagattggt cgcctggaag
    18601 tgatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat ctcagcttgc
    18661 gcaacatgac cttcagtaaa gatgactggc agacacaaga aggcaaactg tcgatgaacg
    18721 ctagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac gcggaatttt
    18781 ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt atggtcagaa
    18841 cgtcagggaa ctggctgcgt gacgggaaaa cgttactcct tgatgatgcg gcaattgccg
    18901 ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg acaccgggtt
    18961 ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc attgatatcg
    19021 accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac ctgacgctgg
    19081 ttaccgatca taaatggggc gtctggagtg gttcggcgaa tctgaatgcc gccgccgcga
    19141 cgtttaatcg tgttgatgtc cgtcgtccat cgctggcgct aaccgccaac agcagcacgg
    19201 tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc accgccagtg
    19261 tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt gtgccggtga
    19321 atattttgca acattgggga tggcctgaat taccgttgac tggcgacggc aatattcagc
    19381 ttaccgccag tggcgatatt caggccaatg tcccgctgaa acctacggtt agcgggcaat
    19441 tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg ggcgtagttt
    19501 ccagtggtga agttacgtcg acggagccgg cgcagtaaaa ataaggacgc tagcggatat
    19561 cagcgctagc gttccccgtt attagcgcaa aattccatac tcactgcaca ataaccattt
    19621 caccggttca tcttcttcta tcgcaggaaa acgtcctccc ggctcaagaa aatagttatc
    19681 gtgttggtca tcatttacca tcgaaatttc ccagccgaga acaaacgctt tttccgccca
    19741 gaagcgatga tacagacgcg gcggcaggca aatgccctgc cccggcttta acaccagcgt
    19801 ttcaccaggt ttcatggtac gccgctgacc atcaacactc acggtaatga ctcgttgatc
    19861 atcacagaga ctggcttcgt tggcccaggc caactgcatg cataagtctc caccgccccg
    19921 attgaggata tcttccattt tgtgggtgtg aaaatgccag ggagtctgct gatcttgttg
    19981 gatctgcaac attttctcag catacggttt tggatatgcc gccgacccca gcgcgccgtt
    20041 gcgcatcgtt agcagtgtta gtcccgtttg gttgaaatca ccacgaccaa aatcggtgat
    20101 atcccaaccg agctgtaagt cgaccacttc ttgccagtta tcccgttccc gctgcagcca
    20161 gtcatccacc gtaaaatagg cgaactctgg caaacaaacg cggaaggtct gcgcaatggc
    20221 atgtgcttta tcaataacat aattaatttg tgaacgtttc attggcttct ccgttgcgca
    20281 tagcaagcca ggcagcgcca cgcattccgc tactgtcgcc gtgctgtgct tttacaatgg
    20341 gagtggtgaa ttgatcggtg aaaaccagtg gagcgacttc tgcgttcaga tcggtgataa
    20401 gcagttccac attcgaaagc ccgccgccga tcacaatcac gccagggtca acaacattaa
    20461 cgatggttgc gagcgtgcgg gcaagctgtt ggcgaaaacg tgccacctgc tgcatggcac
    20521 aagcatcacc acgttgtgcc agggtgacaa tcgccgcagg agtgagcaag ttaccagtca
    20581 tctgttgata gcgttcacta aaaccgctgc cggaaacaaa cgactccacg cagttgtgtt
    20641 tgccgcaata gcaactgact gatggaccat cttcctgctc cgtatagcct ggcaacgtga
    20701 tatgaccgca ttcggcagca tttccccagg cccctataaa cggtcgttgc ttgatggcaa
    20761 tgccaccgcc gcagcccgta ccaagcgtaa taccaaatac cacgtcataa tcttgcccgg
    20821 caccgtcgca agcttctgat agcgcgaaac agttaccatc attggctatc accaccggtt
    20881 gcccaagcaa ttgctgcaaa tcagcctgta acgcatgacc gttaatcacc tgaatattcg
    20941 cgtttttaat caggccagtg agtggcgaga tactgccggg taaggcgatc cctgtcagca
    21001 tcggtcgttg aacgtcccgc ctaatctgct cgataagtgc cacaacgcat gagacaaatt
    21061 gttgatatgt cgacttttgc gtcgggcagc ggtaacggcg aatctcccag ccatgcgcat
    21121 ccatgacgac ggcggcgatt ttggtcccac caatatccaa ccccagatag tgcatatctc
    21181 gttccttgtt gcgtaaaagg cgggtcaccc cgcccttctg gcttagtaca gtgatgcttt
    21241 accgagcgaa ccgaacagtt tcattttgtg gcgaatgact tcccgcgccg cgttaatcgc
    21301 atccggatag atgacgttcg ggtcccacca gctctcttta gcgagaattt cacgcacttt
    21361 ctggaaatag gcgtatttca tatcgctgga gatattgatt ttgccgacgc cgagcgtaac
    21421 tgattcagca atctccgcgt ccgggtttgc cgaaccaccg tgtagtacca gcggaatatc
    21481 aatacgtccg gcaatgtcgc gcaaaatgtt catttgcagc ttcggttgca tcccttttgg
    21541 atagatgcca tgcgctgtac caatcgccac agccagtgtg tcggcacctg tacgagcaat
    21601 aaaatcggcg gcctgcgccg gatctgtgta ggtcacttgt gaaacaccac cttctaccga
    21661 cgttcctgtt tgtccgatgg ttcccagttc accctcaacc gacacgccaa ccgcgtgagc
    21721 gaggcgaacc acttctgcgg tcagcgccac gttttcttca tacggcagca gcgaaccgtc
    21781 gatcattact gaggtaaatc cgcagcgaat cgcacgcaaa acatgctcta cagaggcacc
    21841 atgatcgagg tgcagtacaa acgggactgg actacgctgg gtaatttcgc gaacgtgagc
    21901 gaaaaaggca tcgcccacaa aatcatgttc gctgggatgg atggcaataa tcgccggagt
    21961 atttgtcgct tctgcttcat ccacgactgc gcgaaggaaa cagttatccg ccacattaaa
    22021 tgcgccaatg gcaaagccgt gctggcgggt aggttgcagt aattctttca tcgaaactaa
    22081 catgttaaat ctccagtttc agcgtgtagt ccgccctgcc ggtgcgttat tgcactggca
    22141 taaaggccat gacagaaaat gaatcaggct ttgccgatag agccgctcgc ctgtaaatac
    22201 ggtgtaatcg cttcttcgaa ggccagttcc atagctttca gcgcgtccac cagttcaatg
    22261 gatatatcgt gacagagggt atgcaggagc gcggatttcc cccgttcaaa aatggccgcc
    22321 cccacgttga tttttgctac ccccagcgtc gtacagagct ggagctgatc gaacggcgta
    22381 ccgctaccgc catgtaatgc cagaggcacg ctcgagcagt ggaaaatctc cgccagacgc
    22441 tgaaaatcaa tagcaggtac aacaccaggc gcgtacatgc cgtgcgccgt acccacagag
    22501 acagccagta gatcaatgcc tgtctgcgcg ataaacggtt ccacatcagt gggttgcgtc
    22561 atcatgtctg cattggcggc ctggtcgtaa cgcggcgcat ctgccagatg gcccagttcg
    22621 ccttccacgc agagctgcgc gctggcggcg agttccacca ccgccctggt ctggcgcacg
    22681 ttttcctcca ggggaaaagc agaagcatca atcattaatc cgctgaaacc agcacgaaac
    22741 gcctgacgaa tacagtccgg atttttgcca tgatcaagcg ccagagtgac cggtactgag
    22801 gcagattccg cgagcgtttt aaccactgcc gccgcgacgg gtgcaggaaa atgtgccaga
    22861 tgcccctgat agagattgag aatgatcgga gcgcgattct tctccgccac gcggatagct
    22921 gcgtaagcac tttccagatt catacagttg atcgccagca aggcgtaatg ttcatgccag
    22981 gctttcatca ccatactttt catatcggca aacatagggc ctccggttat tcgagggtga
    23041 aagtgatctc ttcatctttc agcttttgat cgctcagttc gtcaaactct tcgtcgcgtt
    23101 ccgttaccgg tttcttccag agtgaaagca tcacgccaca aatcaccgtg cctataccca
    23161 gcgccaggca aaacagcaga ggatgggtga acagaggcac aacaaacatg ccgccatgcg
    23221 gaaccggact ctccacgccc cacaccatga ttaagccgcc cgcgaccgcc gatgccacca
    23281 cacaagatcc cactactcgc agcaggtcac gcgcggcgat cggaatgact ccttcagtga
    23341 tcatgcagat ccccatcgga aacgcggctt tcagcgcctc gcgctccgca cgggtgtatt
    23401 tatggcgtgt cagtaagaac gaaagtgtga tgccaaacgg agggatgatt gagccgacaa
    23461 atttcacggc ttctggtccg tagacgccac tgaccagcaa gccatcggaa aacagcgaca
    23521 tggttttgtt gaccggacca ccgaaatcga aggttgccat cgcgccaagt atggccccca
    23581 tcaggaagcg cgatccgccc tgcatcgact cgagcaaatg aatcagtgct tcttgtaacc
    23641 aggcgatggg tttaccaatc agcgtcatca ttaacagccc accgataatg gtgcttaata
    23701 ccggcaacac catgattggc atgagtcctt gcatcgactg cggcaggcgg atatagcgtt
    23761 taagtagcaa aatggtgtag cccaccagga aaccacccaa cataccgcca ataaatccgg
    23821 tatggatttg cccacagaga aagccgacaa tcagacccgg ggcaaatccg ggacgatcgg
    23881 caatagaata ggcaattgcc gcgctaatca gcggcacaat cagccccatt ccccagccgc
    23941 caatctgatt aagcatccac gggattgttc ctgttttttc accaacatct gtaccgccaa
    24001 gaacttgccc caacgcaata catatcccgg cggccacaat aaggggtatc atccacgaga
    24061 tgccggttaa taaatgtttt ttaatttcct gtgaaaccgg aattttattg tctttcatat
    24121 taagatccct ccagtataag gatcatttgt ttcccagtga tttttcgacc ttttcaagaa
    24181 attgaacggg cgatttaatg acgatctctg tctttactcg cacaatacgt ttattggtga
    24241 agcgttcttc tccttttatt ttcacatcta cagcaagaat cacgacgtca gcagcagaga
    24301 tatctgccgc ctgtaattca ttttctgtac caatggttcc ctgggtttca actttaatgg
    24361 tatggcccag agcttttgct cctttgatta acttttcacg agcaatatag gtatgagcaa
    24421 tacccgccgt acaggcggca acacagacaa tattcatgac atactccctg ttatttagca
    24481 gacatcggat tgtgcatact gactaaagag atcaatgatt ttttgcggac tcgtctcaac
    24541 taacaattgt tctatgatgt catcatccgc cagcgcgccc gcgacctggg aaagtaaacg
    24601 gatatgggtg gtattttgat cttcaagacg taccgcaaat aaaataatac agcgcacgct
    24661 actaccatcc agcgtttccc agggaatatc ctggcgggta cggccaattg ccagcgtagt
    24721 attaattacc gcagaagatt taccatgcgg gatcgcaata tgattttcaa aaccagtcga
    24781 gccttccgct tcgcgctgcc agacatcttt aataaaggtt tcccgacaat cgatcgcacc
    24841 atcttttttg agaagattag ttaattcttc aatcgcctcc gctttattcg tcgcttgcat
    24901 atctaaaata acgcgattcg tattcaggat ttttgttatg tccattcgtc atacctcata
    24961 aaattctgac attaaatttc aacgacccga gacgtcgcat gatgtaattt ttccgtaatc
    25021 accatttcag tgacccgctt aatatcggca tcattaaaaa aagcggtaac ataagcgacc
    25081 ggcatagtga ctgtaggcaa attaatggtc gaaataatca gttcgatatt gtcaggcaaa
    25141 acctgtgata aattcgctgc tgaaataaca tcaacaatcg tccattcagg aaatgctcgc
    25201 agaatacggc ttttcagtag atgcgaagtt ccgattccgg ttgaacagac cagtaataca
    25261 cgtttacggg caatttgccg ctccatcgca gcctgaaaat gaacggtcag gtaacccact
    25321 tcatcctcgc tcactgcatt ctcaccccag gttttaaata cctgattcac cacatattgc
    25381 gtcaaccgcc agacatccgc taattctgct ttaatatctt ccagcaatgg attacggata
    25441 tgaatacgat aatttagtcg gttaatcagc ggtttaatat gcaccaataa gccatcatat
    25501 aatgcgctgt cttcactaaa atcgcagtcc acgatgtcac tgaataccgt aattaaacgc
    25561 caggtaataa gacgcgcttc attgctggcc tgcatatgtg aaataatgct aacatctttt
    25621 tgtccatcaa tcgccacacc tgatgaaatg atatattgat aaataaacca gacttcatca
    25681 tcgggcaatg tatgtgcaat tcgttgttct atcttatgaa tcatctgact ggcaacatta
    25741 aagatatttt catcatattg cctggtttga tcttcttctc ttgataacgc attcccgtgc
    25801 gtgttgcgat acatcataat aaggatgtga gtaaaaatgt taacgtaata aggttctccc
    25861 aaagaccaac ttaattcatt ttccatatcc agtaacaatg actggacaaa taacacctct
    25921 tcttctccaa aataatggac taatgcttta taacttccgg gatccagacg tgaatacacg
    25981 acaccttgcg gctcattatg gttaataatg ccgttaatca gtaatgccat tgcctgtcgg
    26041 acttgccctt cactaccttc aatatgcgta ccacttgggc tgcggatcaa cgataacccc
    26101 aacggcgcaa gccaggactc aattactctc aggtcattaa caatagaggc tccgctgata
    26161 aagtagcgtt ctgacaattt actgatcgac gtttcattcg gtgtttcact taataactgc
    26221 gacgctattt taacgcgcct ggcattattc attacaccat caatctcttc acattctgct
    26281 cccaacaaat gatcaagatg cagcaaatca gcaatattct ccgcgcttaa catcacaccc
    26341 gcgccagcac gcttctcaac atttattctc cattgcccca gccattgctc aagtaaacgt
    26401 aaatcacgtt gaatggtttt ttccgaaaca tttaaatagc cggcaatctt aacaatagtc
    26461 gtatattccc ttcgaggtaa aaggaatcgt aataatctgt tttgtcgcga ggtaatcatt
    26521 tgcatttttt acccttatca cagattaaga agccccatga ggaagagctt cagttgccat
    26581 ctctgagggt aaatataaag cagaataatt tcacaacaat actaaaatct gtccgtaatc
    26641 cggacagtga taaaacaaag caaggcggtt aagggatatt tttcagcaca gatcacattt
    26701 tcgcgatgaa taatcgtgat catgctcgca aatacgatgg aagaaatatt tattaccaga
    26761 tgtgagatta tttaccggat acgacacgaa accattcgta tccggcattc ttcaatagat
    26821 tacaacgtaa ttgtcagcgc attcccttga ggcgtcacca ccagcccctg ctcactttca
    26881 gcctgcgaac cgccttgcag accatttact ttcacaatat tgcgcaagca cagtgtccag
    26941 ttcctcacct cgcccgcacc agtcacagta atcgtgttac cagtacgtgt tgcttttaga
    27001 gtaaagatca ccgaaccgtc agcagcgggc acttcacaga cggcttcatg cccgtcttgc
    27061 agattaaaga gctggaatgc cgtgccttcg tgccacgcgt aatcgggacg ttgatcgttg
    27121 ttgcccagcg ccagtagggt gttatcacgc acataaatgg gcagactcag gaagctgtgc
    27181 tgctgtttat gccagcgact accgtcgagc tcatcgttgt gccacaggtg tgtccagcga
    27241 ccttctggca ggtagaactg cacatcgccc gcttcagtga acaccggcgc aaccatcacg
    27301 ttgtcgccta acatgtattg acggtcaagg taatcacaag ccggatcgtc cgggaactcc
    27361 atcatcatgg cccgcatcat cggcgtaccc tgcgcgttcg cacgagcagc ttcacgatac
    27421 agatacggca tcatgcggca tttcagttgc gtgaagaagc gcaccacatc acaggactca
    27481 tcatcgtagg cccacggcac acgataagat ttgctgccat gtaaacgact atggctggag
    27541 agcaaaccaa acgcgcacca gcgtttgtaa acgtgcgccg gagcggtatt ctcaaagccg
    27601 ccgatatcgt ggctccagaa gccaaaacct gaaaggccaa tagacaaacc accgcgcaga
    27661 ctttccgcca ttgattcgta gttagcgtaa cagtcacccc cccaatgtac cgggaatttc
    27721 tgcgcaccga cggaggccga gcgggcaaac aagacggctt cttcctctcc gacggtttcc
    27781 ttgagcacgt tccacaccag ttcgttgtag atgtacgcat aatggttatg cattttctgc
    27841 gaatcggaac cgtcaaacca ttgaacatcg gtaggaatgc gttcgccaaa gtcggtctta
    27901 aagcaatcaa cgcccatcgc gaccagacct ttcagtttgt cggcgtacca tttgcaggca
    27961 tccggattgg taaagtcata aatcgccaga cctggctgcc atttatccca ctgccacagc
    28021 gaaccgtccg ggcgtttgag taaataacct ttctcttgta actctttaaa gacgggggat
    28081 ttttgcccaa tataggggtt aatccagacg cagattttca gccctttcgc tttcagacgg
    28141 cggatcatcc cttccgggtc cggaaaagtc agcgggtccc actcaaaatc gcaccactgg
    28201 aaggctttca tccagaaaca gtcaaagtgg aaaacatgca gcggcagatt gcgttccgcc
    28261 ataccatcaa taaagctatt taccgtcgct tcgtcgtagt tggtggtaaa tgaagtggtt
    28321 agccacagac cgaaggacca cgcgggcggc agcgccggac gaccagtaaa gcgggtataa
    28381 cgatcaagta ccgctttcgg cgtcgggccg tcgataacaa agtattcgag atattcactc
    28441 tcaacgctga actgtacttt ggagactttc tccgatccca cttcaaaaga gacgcattgg
    28501 ggatgattga ccagtacccc ataaccacgg ttagtcatat aaaacgggat atttttatac
    28561 gcctgttcag tacttgtgcc gccgtcccgg ttccaggtct ctaccgtctg accattgcgc
    28621 accagggcag taaagcgctc tcccaggccg taaactgttt cgccaacgcc aagatcaagc
    28681 cgttcaaaca tataatttcg ttgattattc gtgtcctgca cgtagccatt atttttcacc
    28741 tgactaccgg taatacgttc gccgttgcgc agaaaatcca gtgaccagaa ctcacctttg
    28801 ctgacacgcg cgcttaagtt gccactttta aactcagcat aacgttctgt gttttcgatt
    28861 gtgaccttca cgtcctgcaa aatattgagc ggataatgag ggccgttatt cagcgccccc
    28921 tgaaaatgct caatccgcac cccgacaata ccttcctgtg gggagaaaaa gcgcagcgta
    28981 aataaaggcg tgtcaagctg ccaggtacgt tcacgtacat cacggggggc agcatagacc
    29041 accatttcat taccctgctg ttcaacctcg aatacctgaa gcgggtgaat caaattgagg
    29101 ccaggttgaa tcaaccagtt tccatcgcta attttcattg ccgttcctta gttctgcact
    29161 tcttgagagg tgaattgttg ctggcaacgg cgtttaccct gagccagctg ttccataacg
    29221 gttttcagat tgtgcgtcgt gagtgagtag tagcgtttag cgacaatcgc gctcagcaaa
    29281 taacagatcg ccggaacaat cgtgaatagc gcaataatga tgctaatcgt ggcgctgttc
    29341 tgcgcttttt ctgccgcatc atatccgcca taagccagca tccagccgat aagagcgccg
    29401 ccgaaggcca accccagttt gagcacaaac agcgtgccag caaaactgat cccggtcagg
    29461 cgtttaccat tgcaccactc gccgtagtcg acggtatcgg acatcattac ccactggata
    29521 ggtgtcacca gttgatgcaa cacaccaatc acgaagatga agacaaacat agtgatgctg
    29581 gcctgcatgg gaacaaagaa catcgcgatg ctaatcactg ccagcagggc gttcgtccac
    29641 cagaagatag tgactttaca tttccagtcg gtcagcggtt ttgccagtgc ggaaccaatc
    29701 aagttaccca cgcaataagt ggtgagaaaa gcgacaaaca cttccggcgt acccaaaatc
    29761 catgtgacgt aatacatcat cgctccaccg cgtacgcaca cagccaggat attgaaaatg
    29821 gttagtaaac cgacaatccg ccactggtcg ttttgccaga tatcacgtaa atcttcccgc
    29881 atagacgtcg ttgtaggtgg tgcttcaacg cgttctttag tggtgaagaa acagaatgcc
    29941 agcatcatga atgccaccac ggaaaggacc gcgataccgc cctggaaacc gagtggttta
    30001 ttatcaccac caattaaatt aaccaatggc atcatcagaa ccgtagaaag catgcctccc
    30061 gcggtcgcca gcacaaaacg ccaggattgc agcgagatac gctgcgtcgg gtcattggtg
    30121 attacaccac ccaatgcgca ataagggata ttaacgacgg tataaagtaa ggtaagtagg
    30181 gtgtaagtaa ttgctgcata aatcattttt ccgttcatac tgagatctgg cgtgctatag
    30241 gccagtacac agacgatccc gaatggcagt gcgccaaaca gtacccacgg acgaaattta
    30301 ccccagcgag agcgcgttcg atcggccaac aaccccatgc aaggatcgga aatcgcatcc
    30361 agtgcacgag cgaccaaaaa catagttccg acaaatccgg caggaatgcc aaaaatatcg
    30421 gtataaaaga acatcatata taacattacg ttatcgaaaa taatgtggct ggcggcgtct
    30481 cccatgccat aaccaatttt ctctttaacg gacaacactt cactcttcat ctgcttttcc
    30541 ttgatgctat aggggtgctg agaccgataa cgcattttgt aaaccatccc tgtgagagcg
    30601 aatattccga tatctggtta ttaatttatg attcttgttt tatgtgatcg tggtagcgtt
    30661 aattccgctc atatatcatt gtaaaatatg ggttttatat gaactataat gctttcgtga
    30721 taatactctg cgtgtattag gcggaaaaaa ctgatctggg ggatgtagaa actcaaggaa
    30781 gtagctataa tgcgccccgc ctccatgtag caatcgaggc gcggaagatc gtcgtctccg
    30841 gtgaggcggc tggacttcaa atccagttgg ggccgccagc ggtcccgggc aggttcgact
    30901 cctgtgatct tccgccaaaa tgcctcttca tacgttctcc aacccctcgt aatactgtca
    30961 atccagatat tttatactgc tcaatattca atcggcttca ggaatacctg gtcaaaggtt
    31021 ttgtgatgga cgatgagcag ctgaagaatc cgcctgtcgg ttcttctgtc ctcgaacgca
    31081 tccgcgatat tcgcgccagc atgcgccgtg tttatttaca ggtgattttc ccccctgctg
    31141 actctcagcc aactgtatcg ccattaaggt gaagatgttg ttttacacgc tccagaacac
    31201 ccttttttta tacgactgtt ctattccttt aacagctggc aaaaaaactg acctataaac
    31261 ttcaccaata atcagactgc catcaagatt aatttgttca tttaatatat caaatcaact
    31321 tgttttattt ttactatcaa aaatagct
//
LOCUS       DAZKFU020000028        31258 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZKFU020000028 DAZKFU020000000
VERSION     DAZKFU020000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.028"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            452..2041
                     /gene="purH"
                     /locus_tag="ACHOQQ_RS05195"
                     /old_locus_tag="pgaptmp_001039"
     CDS             452..2041
                     /gene="purH"
                     /locus_tag="ACHOQQ_RS05195"
                     /old_locus_tag="pgaptmp_001039"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGU4469320.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            2053..3342
                     /gene="purD"
                     /locus_tag="ACHOQQ_RS05200"
                     /old_locus_tag="pgaptmp_001040"
     CDS             2053..3342
                     /gene="purD"
                     /locus_tag="ACHOQQ_RS05200"
                     /old_locus_tag="pgaptmp_001040"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGU4469321.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(3339..4664)
                     /gene="zraR"
                     /locus_tag="ACHOQQ_RS05205"
                     /old_locus_tag="pgaptmp_001041"
     CDS             complement(3339..4664)
                     /gene="zraR"
                     /locus_tag="ACHOQQ_RS05205"
                     /old_locus_tag="pgaptmp_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGU4469322.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(4661..6037)
                     /gene="zraS"
                     /locus_tag="ACHOQQ_RS05210"
                     /old_locus_tag="pgaptmp_001042"
     CDS             complement(4661..6037)
                     /gene="zraS"
                     /locus_tag="ACHOQQ_RS05210"
                     /old_locus_tag="pgaptmp_001042"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGU4469323.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            6275..6700
                     /gene="zraP"
                     /locus_tag="ACHOQQ_RS05215"
                     /old_locus_tag="pgaptmp_001043"
     CDS             6275..6700
                     /gene="zraP"
                     /locus_tag="ACHOQQ_RS05215"
                     /old_locus_tag="pgaptmp_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGU4469324.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(6702..7397)
                     /gene="yjaH"
                     /locus_tag="ACHOQQ_RS05220"
                     /old_locus_tag="pgaptmp_001044"
     CDS             complement(6702..7397)
                     /gene="yjaH"
                     /locus_tag="ACHOQQ_RS05220"
                     /old_locus_tag="pgaptmp_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGU4469325.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(7410..7682)
                     /gene="hupA"
                     /locus_tag="ACHOQQ_RS05225"
                     /old_locus_tag="pgaptmp_001045"
     CDS             complement(7410..7682)
                     /gene="hupA"
                     /locus_tag="ACHOQQ_RS05225"
                     /old_locus_tag="pgaptmp_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGU4469326.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            complement(7869..8459)
                     /gene="yjaG"
                     /locus_tag="ACHOQQ_RS05230"
                     /old_locus_tag="pgaptmp_001046"
     CDS             complement(7869..8459)
                     /gene="yjaG"
                     /locus_tag="ACHOQQ_RS05230"
                     /old_locus_tag="pgaptmp_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGU4469327.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            complement(8502..9173)
                     /gene="nfi"
                     /locus_tag="ACHOQQ_RS05235"
                     /old_locus_tag="pgaptmp_001047"
     CDS             complement(8502..9173)
                     /gene="nfi"
                     /locus_tag="ACHOQQ_RS05235"
                     /old_locus_tag="pgaptmp_001047"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGU4469328.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            complement(9183..10247)
                     /gene="hemE"
                     /locus_tag="ACHOQQ_RS05240"
                     /old_locus_tag="pgaptmp_001048"
     CDS             complement(9183..10247)
                     /gene="hemE"
                     /locus_tag="ACHOQQ_RS05240"
                     /old_locus_tag="pgaptmp_001048"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGU4469329.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGDVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            complement(10287..11060)
                     /gene="nudC"
                     /locus_tag="ACHOQQ_RS05245"
                     /old_locus_tag="pgaptmp_001049"
     CDS             complement(10287..11060)
                     /gene="nudC"
                     /locus_tag="ACHOQQ_RS05245"
                     /old_locus_tag="pgaptmp_001049"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGU4469330.1"
                     /translation="MDRIIEKLDHGCWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            11155..11631
                     /gene="rsd"
                     /locus_tag="ACHOQQ_RS05250"
                     /old_locus_tag="pgaptmp_001050"
     CDS             11155..11631
                     /gene="rsd"
                     /locus_tag="ACHOQQ_RS05250"
                     /old_locus_tag="pgaptmp_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGU4469331.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     regulatory      11683..11783
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11864..13759
                     /gene="thiC"
                     /locus_tag="ACHOQQ_RS05255"
                     /old_locus_tag="pgaptmp_001051"
     CDS             11864..13759
                     /gene="thiC"
                     /locus_tag="ACHOQQ_RS05255"
                     /old_locus_tag="pgaptmp_001051"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGU4469332.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGAHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     gene            13759..14394
                     /gene="thiE"
                     /locus_tag="ACHOQQ_RS05260"
                     /old_locus_tag="pgaptmp_001052"
     CDS             13759..14394
                     /gene="thiE"
                     /locus_tag="ACHOQQ_RS05260"
                     /old_locus_tag="pgaptmp_001052"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGU4469333.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQTADWRLATAQLLEIAGVGDE"
     gene            14387..15142
                     /gene="thiF"
                     /locus_tag="ACHOQQ_RS05265"
                     /old_locus_tag="pgaptmp_001053"
                     /pseudo
     CDS             14387..15142
                     /gene="thiF"
                     /locus_tag="ACHOQQ_RS05265"
                     /old_locus_tag="pgaptmp_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            15126..15326
                     /gene="thiS"
                     /locus_tag="ACHOQQ_RS05270"
                     /old_locus_tag="pgaptmp_001054"
     CDS             15126..15326
                     /gene="thiS"
                     /locus_tag="ACHOQQ_RS05270"
                     /old_locus_tag="pgaptmp_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGU4469334.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINHQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            15328..16098
                     /gene="thiG"
                     /locus_tag="ACHOQQ_RS05275"
                     /old_locus_tag="pgaptmp_001055"
     CDS             15328..16098
                     /gene="thiG"
                     /locus_tag="ACHOQQ_RS05275"
                     /old_locus_tag="pgaptmp_001055"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGU4469335.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            16095..17228
                     /gene="thiH"
                     /locus_tag="ACHOQQ_RS05280"
                     /old_locus_tag="pgaptmp_001056"
     CDS             16095..17228
                     /gene="thiH"
                     /locus_tag="ACHOQQ_RS05280"
                     /old_locus_tag="pgaptmp_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGU4469336.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(17638..18176)
                     /gene="yjaZ"
                     /locus_tag="ACHOQQ_RS05285"
                     /old_locus_tag="pgaptmp_001057"
                     /pseudo
     CDS             complement(17638..18176)
                     /gene="yjaZ"
                     /locus_tag="ACHOQQ_RS05285"
                     /old_locus_tag="pgaptmp_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
     gene            complement(18389..22612)
                     /gene="rpoC"
                     /locus_tag="ACHOQQ_RS05290"
                     /old_locus_tag="pgaptmp_001058"
     CDS             complement(18389..22612)
                     /gene="rpoC"
                     /locus_tag="ACHOQQ_RS05290"
                     /old_locus_tag="pgaptmp_001058"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGU4469337.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(22689..26717)
                     /gene="rpoB"
                     /locus_tag="ACHOQQ_RS05295"
                     /old_locus_tag="pgaptmp_001059"
     CDS             complement(22689..26717)
                     /gene="rpoB"
                     /locus_tag="ACHOQQ_RS05295"
                     /old_locus_tag="pgaptmp_001059"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGU4469338.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            complement(27037..27402)
                     /gene="rplL"
                     /locus_tag="ACHOQQ_RS05300"
                     /old_locus_tag="pgaptmp_001060"
     CDS             complement(27037..27402)
                     /gene="rplL"
                     /locus_tag="ACHOQQ_RS05300"
                     /old_locus_tag="pgaptmp_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGU4469339.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            complement(27469..27966)
                     /gene="rplJ"
                     /locus_tag="ACHOQQ_RS05305"
                     /old_locus_tag="pgaptmp_001061"
     CDS             complement(27469..27966)
                     /gene="rplJ"
                     /locus_tag="ACHOQQ_RS05305"
                     /old_locus_tag="pgaptmp_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGU4469340.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            complement(28258..28962)
                     /gene="rplA"
                     /locus_tag="ACHOQQ_RS05310"
                     /old_locus_tag="pgaptmp_001062"
     CDS             complement(28258..28962)
                     /gene="rplA"
                     /locus_tag="ACHOQQ_RS05310"
                     /old_locus_tag="pgaptmp_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGU4469341.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            complement(28966..29394)
                     /gene="rplK"
                     /locus_tag="ACHOQQ_RS05315"
                     /old_locus_tag="pgaptmp_001063"
     CDS             complement(28966..29394)
                     /gene="rplK"
                     /locus_tag="ACHOQQ_RS05315"
                     /old_locus_tag="pgaptmp_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGU4469342.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            complement(29553..30098)
                     /gene="nusG"
                     /locus_tag="ACHOQQ_RS05320"
                     /old_locus_tag="pgaptmp_001064"
     CDS             complement(29553..30098)
                     /gene="nusG"
                     /locus_tag="ACHOQQ_RS05320"
                     /old_locus_tag="pgaptmp_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGU4469343.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            complement(30100..30483)
                     /gene="secE"
                     /locus_tag="ACHOQQ_RS05325"
                     /old_locus_tag="pgaptmp_001065"
     CDS             complement(30100..30483)
                     /gene="secE"
                     /locus_tag="ACHOQQ_RS05325"
                     /old_locus_tag="pgaptmp_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGU4469344.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            complement(30713..>31258)
                     /locus_tag="ACHOQQ_RS05330"
                     /old_locus_tag="pgaptmp_001066"
     CDS             complement(30713..>31258)
                     /locus_tag="ACHOQQ_RS05330"
                     /old_locus_tag="pgaptmp_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HGU4469345.1"
                     /translation="PIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCT
                     GVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKD
                     EGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGL
                     RFAIREGGRTVGAGVVAKVLS"
BASE COUNT         7239 a         8547 c         8103 g         7369 t
ORIGIN      
        1 cactgtcgcg aggtggcgta tattacgctt tcctctttca gagtcaaccc tgaatttcag
       61 ggtttttctc ttcaaccgaa ccggctgttt gtgtgaagtg attcacatcc gccgtgtcga
      121 tggaggcgca ttatagggag ttctccgcag gtcgcaatag aaaaattgca taaaaataac
      181 tgactgctgc attccccagc aaaaccccgc tttatacctt tttacacaca gagttatcca
      241 caatcatcag cgttattttt gtatttttca cacggtaacc actgtcaaaa ttgtgatcac
      301 cattgaaaga gaaaaattcg cgagcgttgc gcaaacgttt tcgttacaat gcgggcgaaa
      361 aataaggatg ccccgttagg ggcgttagct gagtttttcg cgaaaaattc agctaacgct
      421 ctctgtaata gtcaaatcca ggggatttac catgcaacaa cgtcgtccag tccgccgcgc
      481 tctgctcagt gtttctgaca aagccggtat cgtcgaattc gcccaggcac tttccgcacg
      541 cggtgtggag ctgctgtcta cagggggcac tgcccgtctg ttagcagaaa aaggtctgcc
      601 ggtaaccgaa gtttccgatt acaccggttt cccggagatg atggatggac gcgtgaagac
      661 cctgcatccg aaagtacatg gtggcattct gggccgtcgc ggccaggacg atgccattat
      721 ggaagaacat cagatccagc ctatcgatat ggtggttgtt aacctgtatc cgttcgccca
      781 gacagtggcc cgtgaaggtt gctcgctgga agatgcggtt gagaacatcg atatcggcgg
      841 cccaacgatg gtgcgctccg ccgccaagaa ccataaagat gtcgcaatcg tggtgaagag
      901 cagcgactac gacgccatta ttaaagagat ggatgacaac gaaggatcgc tgacgcttgc
      961 aacccgtttc gacctcgcca tcaaagcctt cgaacacact gccgcctacg acagcatgat
     1021 tgccaactac ttcggcagca tggttccggc ttaccacggt gaaagcaaag aagccgccgg
     1081 tcgcttcccg cgcacgctga acctgaactt cattaagaag caggatatgc gctacggcga
     1141 gaacagccac cagcaggctg ccttctatat agaagagaat gtgaaagaag cctccgttgc
     1201 taccgcaacc caggttcagg gtaaagccct ctcttataac aacatcgccg ataccgatgc
     1261 ggcgctggag tgcgtgaaag agttcgccga gccggcatgt gtgattgtga agcacgccaa
     1321 cccttgcggc gtggctatcg gcaattccat tcttgatgct tacgatcgcg cgtacaaaac
     1381 cgacccgacc tccgcattcg gcggcatcat tgcctttaac cgcgagctgg atgcggaaac
     1441 cgcacaggcc atcatttctc gtcagtttgt tgaagtgatt attgcgccgt ccgccagcga
     1501 agaagccctg aaaatcaccg ccgccaaaca gaatgtacgc gttctgacct gcggtcagtg
     1561 gggcgagcgt gttccgggcc tcgatttcaa acgcgtgaac ggcggtctgc tggttcagga
     1621 tcgcgatctg ggcatggtcg gtgcggaaga actgcgcgtc gtcacccaac gtcagccgac
     1681 cgaacaggaa ctgcgtgatg cgctgttctg ctggaaagtg gcgaagttcg tgaaatccaa
     1741 tgctatcgtc tatgccaaaa acaatatgac catcggtatt ggcgcgggcc agatgagccg
     1801 cgtgtactcc gcgaaaatcg ccggtattaa agctgccgat gaaggcctgg aagtgaaagg
     1861 ttcctcgatg gcttctgacg cgttcttccc gttccgcgac ggtattgatg ccgccgcggc
     1921 tgcgggcgtg acctgtgtaa tccagcctgg cggttctatc cgtgatgacg aagtgattgc
     1981 cgccgccgac gagcacggta ttgcgatgct cttcaccgac atgcgccact tccgccatta
     2041 atggagcaat agatgaaagt attagtgatt ggtaacggcg ggcgcgagca cgcgctggcc
     2101 tggaaagcgg cccagtcgcc gctggttgag actgtttttg ttgctccggg taatgcaggc
     2161 actgcgctgg aacccgcgct gcaaaacgtt gctatcggcg tgaccgatat cccggcgctg
     2221 ttggatttcg cacaaaacga aaagattgat ctgaccatcg tcggcccgga agcgccgctg
     2281 gtgaaaggcg tggtcgatac cttccgcgct gccgggctga aaatcttcgg cccaaccgca
     2341 ggtgcggccc aactggaagg ctcaaaagcg tttaccaaag atttcctggc ccgccataag
     2401 atccctacgg cggaatacca gaacttcacc gaggtagaac ctgcgctggc gtatctgcgt
     2461 gagaaaggcg cgccaatcgt cattaaagcg gacggtctgg ctgccgggaa aggcgttatc
     2521 gtggcgatga cgctggaaga agcggaagcg gctgttcacg atatgctggc gggcaacgct
     2581 tttggcgacg cgggtcatcg catcgttatc gaagagttcc tcgatggcga agaagcgagc
     2641 tttatcgtga tggtggacgg cgagcatgtg ctgccgatgg ctaccagcca ggatcacaaa
     2701 cgcgtaggcg ataaagatac cggaccaaac acgggcggga tgggcgctta ctcccccgcg
     2761 ccggtagtaa ccgatgaagt tcatcagcgc accatggaac gtattatctg gccaaccgtg
     2821 aaaggcatgg cggcggaagg caacacctac accggttttc tctacgcggg cctgatgatc
     2881 gacaaacagg gcaatccgaa ggttattgaa tttaactgcc gctttggcga tccggaaacc
     2941 cagccgatta tgctgcgcat gaagtccgat ctggttgagc tctgcctggc ggcctgtgaa
     3001 ggcaaactgg acgagaaaac gtcagagtgg gatgaacgtg cttctctcgg cgtggtgatg
     3061 gctgcgggtg gatatccggg cgattaccgc accggcgatg tgatccacgg cctgccgctg
     3121 gaagaagtgg ctgacggcaa agtgttccac gcgggcacga aactggcgga taacgagcag
     3181 gtagtgacca gcggcgggcg cgtactgtgc gtcaccgcgc tgggtcatac cgtggcagaa
     3241 gcgcagaaac gcgcttatgc cttaatgacc gatattcact gggacgactg cttctgccgg
     3301 aaagatatcg gctggcgcgc tatcgaacgc gagcagaact aacgcgacag ttttgccaat
     3361 agcgttttgc gcgtgatccc taactgacgg gcggcttcgg ttttgttgcc gcccgttttc
     3421 tccagtgccg ccagaatcac ttctttttcg acttccacca gcggctgaat atcctgactt
     3481 tgccccagcg ggatcggcgt accggcaatc gccagcggca gctcgcgttc ggaaatatat
     3541 tccccggtca gcagcaccac cgcccgttcc accgcgtttt ccagttcacg aatatttccc
     3601 ggccagtcgt agtggatcag caaatccatc gcctgtggcg taaagccttt taccgccttg
     3661 cgattacgct cggcaaagcg ctgaagaaaa tggccagcca gcagagggat atcttcccgc
     3721 cgttgccgca gcgatggtac ttcaatcgcc accacattca ggcgatagta gagatcctgg
     3781 cgaaaacgcc cggcattcac ctctgcggca agatcgcgat gggtcgccgc aatcagccgg
     3841 acatcaaccg agatagtctg gttgctaccg acacgctgaa cttcgcgctc ctgaatcgca
     3901 cgcagcagac gcacctgcat catcggcgag atatcgccaa tttcatcgag aaacagcgtg
     3961 ccgccgtccg cctcaacaaa gcgcccttcc cggcgtttat cggctccggt aaacgcccct
     4021 ttttcgtgac cgaacaattc agattccagc aaggattcgt taagtgccgc acagttgagc
     4081 gttaccagcg gtttttcgct acgtgcgcta ctggcgtgaa tcgccctggc gaccagctct
     4141 ttaccggtgc cggaatcgcc gtggatcagc accgtggctt ccgatggcgc gacgagggcg
     4201 atttcactga gcaggtgttg catcgccggg cttttaccga ccataccgaa ctggctggca
     4261 gtcaccgcag gtgtttcagc atcaatactg tgcgtatgcg cgagcgcctt ttccagcgtc
     4321 gcctgtaggt tatcgaaatc cagcggcttg atgagataat ccagcgcccc agttttcagt
     4381 gcctctaccg ccgtctcgac gctggagtac gcagtcataa tcagcaccgg aattgccggg
     4441 tttaacgctt tgatctcttt cagcgtggcg atgccgtcca tctccgccat tcgcacatcg
     4501 caaagcacaa gatcaaaaac ctgttcccgc acctgctcca gcgcctgtcg cccgctgttc
     4561 gccagcgcga cgttatagcc ccagccgcgc agtaaagcct gcaaaatagt gcagtggcta
     4621 atgtcatcat ccaccaccag aatatcgata ttatcgtgcg tcatccttgt gggtccttac
     4681 gcgtaatatt gaccggaagc cagagggtga acgttgagcc ttttccctcc tggcttgcga
     4741 cctgaattgt accaccgtgt tgttcaacaa tattatgcac gaccgccagc cccagtccgg
     4801 tgccttcggc tttagtggtg aagtacggag tgaagatggc atcaagctga tctgccgcaa
     4861 ttcccttacc gctgtcggta acgctgattt tcacgcccgc gccgctttcg ctggccgtca
     4921 cgctaatcac gccatgctga ccaatcgcct gaatagcatt gagatagaga ttcaacagga
     4981 cctgagtcag cctgtccgga tcggcctgaa tttccggtaa tgtgtcgttg gcggtaaagc
     5041 gtaactggat ctcccggctg tttgcatcct ggcttaccag ctgtaatgag tggttaatca
     5101 gcgtgttgag atccaccgcc tgcaaagcca gatgcgttgg cttaaccagt tccagcaact
     5161 cgcttaccac gcggtttaaa cggtcggcct ctttcgccat cacctgcgcc agttgatgcg
     5221 cttctccccc tgcaggcgcg cgctcggcaa agtattttgc cagtccttta atcgaggaaa
     5281 gtgggttacg gatttcgtgg gcaacgcctg ccgcaagatg ccccagcgcc accagcttct
     5341 ctttgcgctt catttcatct tgtagaagct ggcgcgagcg cagatagcgg cgataccaga
     5401 agaatgacaa tacgcttgcc agcaagaccg tcgccagggc gaagaggata atcagggtgt
     5461 ttctctgctc acgatcttcg gcactcacaa tattactggc gtcgaaagcg atgaaaattg
     5521 cttgtggtgt cgcggcatac tgctgcatat ggcgcatccg gtgcattcca gcagcaaaca
     5581 tcggttgaaa ctggcgataa atttccagaa caggctcacc gtctgcgcta tcgatccgcc
     5641 gccacgcttc ttcatctccc ggatgtaact gctgcatttc ctgcggggaa taaagctgtt
     5701 ttcccaccat gccggagttg ctatgcatca cgattgttcc ttgttcatcc gtgaccgcaa
     5761 accaacgtac tccaggctgc ccggccattt cttccagtaa ggcctgctgc tgcgcatgat
     5821 gcatgcgcat ccccatgccg acgcgcgagc cggattcgag agcacgaata agtacactgc
     5881 ctttttccag cagcgtttgt ctggcagcgg cagtctcgcg cccataatcg cgaatcaccg
     5941 tcaccgcaaa caaccctacc agcccaacaa tgaccacggg gaggatcgcg cttaaccatt
     6001 tagctaagga gtctttagaa cgttgcataa aacgcatctt cttctttgcc tgctcatccc
     6061 tttttgacag cagaaatcat gccatctttt atcagcgctt accctgcgct gtaacacaaa
     6121 ggcttaagtt tcaatgagta aaaatgactc gctacccgca gcaggcgagt catttttact
     6181 cgtttatcat gccagattac ccgtcatatc agcgtttcat cgttggcacg gaagatgcaa
     6241 tacccgaagt aagacaacca ctggaggatt aaccatgaaa cggaacacga aaattgccct
     6301 ggtgatgatg gcgctttcag caatggcgat gggatcgaca tctgcatttg ctcacggcgg
     6361 acacggtatg tggcagcaaa atgccgcgcc tttgaccagc gaacaacaga cagcgtggca
     6421 gaaaatccat aatgactttt acgctcaaag cagcgcactg caacagcaac tagtgacgaa
     6481 gcgttatgaa tacaatgccc tgttagccgc gaacccaccg gatagcagca aaattaacgc
     6541 ggtcgccaaa gagatggaga atttgcgtca gtcgttagat gagttacggg tgaaacggga
     6601 tattgcgatg gctgaagcgg gtattccgcg cggagccgga atgggcatgg gctacggcgg
     6661 ctgcggtggt ggcggtcata tgggtatggg ccactggtaa atcagaacgt tttctcgttg
     6721 ggttgccagc gacagaaatc agagtttgcc actaacagta attgcgaacc ttcgggcgct
     6781 tccagccatg ccacgctgac atcaacagaa gagcggctct ggcggcgctc gatatgcgct
     6841 atcgcctgcg aatctaaatc aggtttaatg gtttgccctt cgcaggtggt cacggtctgt
     6901 tccatcgcat gccagcgccc ctggcgcagc acgactctcc cctgacgtaa ggcatcgcta
     6961 atctggcgga tctgatcagc ccgataacgg tataaatcga tttggtcgtt agaaagctgc
     7021 tgcttttccc catcggattc acgttgcata aagctcagat caccgcgatc gtcaaaacgc
     7081 gcgcgaatgt tcaccggcgg tttgctgtaa acattgatat tgatgagcgt gaggttatcg
     7141 ccctgccagc gatactctcg cgtcgtggta tcgccactgc gccatgggct aaacacggca
     7201 agcagatgca cattatcgcc gctgtctttt cgccagatgc gtaccgcgcc ctggtcttca
     7261 gcaaatccac tggcggtaaa gggaggaagt gacgagttgt gactacaggc agtgagaaac
     7321 agcgcacctg ccagcatcaa tgatcgacgc cagaaagaca aaaggggtga aaccacccct
     7381 tcgttaaaac tgttcactgc cacgcaatct tacttaactg cgtctttcag tgccttgcca
     7441 gaaacaaatg ccggtacgtt agctgcggca attttgattt ctttaccggt ctgcgggttg
     7501 cggccagtac gctcagcgcg gtggttcact ttgaaggtac cgaaaccaac cagttgtaca
     7561 gcatcgcctt ctttcagaga ctcagtaatt gcagccagag tggactccag agcagcttta
     7621 gcctgggttt tggacagttc tgctttctct gcaattacat caatcagttg agtcttgttc
     7681 ataagttatc cttacaatgt gtttatcgct tgctaagcat cgagtgcgac ggaaatgcca
     7741 gaatagcact ctcctgcata cacgcaccga tagccacttt ttttcgcccc ccaaatgtag
     7801 accagacggg gggcgaaagg gaagactcca ggtatgacaa atcaggcgtt aaatcacgtt
     7861 ttctggtctt attgctgaaa aattatacca atattgctct cacccgcctc acgcaggtct
     7921 gccctaaggc cttttatcag ctcgatatcg cgttcttcgc actcagctaa aagtcggaat
     7981 atttcccact gaatgtccca ttcttgctct acagctgggt tttctttgag ctcttcatcg
     8041 ctcatttcgc gaccagcctg agtcatttcc agcatcgcaa cggtcgtgat ggaggtctta
     8101 ctcacttcca ccgcgtgttc gagcgtttca ccactcaaac gcgaatggac cagttcactt
     8161 aacgccacgc aggcatcgat tgccggataa acgccgtaca gatcgaaatc gtcggctgaa
     8221 ggaatcgctt cttcaaattt ctccagttgg ctgtcgaaat ttacttttgc gtctttgacg
     8281 gtcagcgttt cccagatgag atcgagaata cggcggtaaa tttgcccatc gccaaaaccg
     8341 gtttgctggc agaacatggc gtaattgggg tacatgcgtt cgcataagca agccatgaaa
     8401 gtgacatgct gccagctttc caggcgctcc agacgcagat gaattgggtt ttgtaacatg
     8461 ttgagttctc aaatacggaa attacccgca gtttacctga attagggctg atttgctgta
     8521 tagcgcacga acgctggacg ttccgaggcc accgcgtccg cccagcgcgt tggctccggc
     8581 agacgatagc ctttcatgca gcgttgtacc cacgccagcg cgctgtccac gctgacccga
     8641 tggccggtag cgataaacaa cgggttacag cgcgctttgc tgcgccagac ccaggccagc
     8701 tgctcgcctt tatccatcag tggggccagc gcgcccggtt cgctggagag cggttcgaat
     8761 ttaccgcata gtcgtttttt cgctacgcca atggtcggca catccaccag caaaccaaaa
     8821 tggctggcga cgccaagacg gcgaggatgc gagatcccat gaccatcgac aaacactaaa
     8881 tccggttttt gtgacagcat ctcccacgct gccaggagcg caggatattc gcggaaggaa
     8941 agaaaacctg gaatgtaagg catggtggta gcgatgcggg ccactttata ctccaccagc
     9001 tcaagcgagg ggtatttcag cagcaccatc gccgctcgcg tcacttctcc gccctgctca
     9061 aacccgacat cggctccggc gatcagatcc ggcggatcct tatcgagtcg atcctcgcgg
     9121 atcacagaag aagccagttc gatttgttga gcgcgtaatg acgcgagatc cataatcgct
     9181 ccttagcggt gatactgttc agacagtcga tgcactgcct ccacgaacac gccagcatgt
     9241 tctggcggca catcctgatg aatgccgtga ccaaggttaa agacatgacc ttcgccgtga
     9301 ccgaaacctg caagtatagt cgctacttct tcttcaatgc gggcaggcgg cgcgtacagc
     9361 atcgacggat ccatattacc ctgcaacgcg actttattgc ccacacggcg gcgcgcatcg
     9421 gcgatgtccg ttgtccagtc gaggcccagc gcatcgcaac cggtttctgc catggcttcc
     9481 agccactgtc cgccgccttt ggtaaacagc gtgaccggta cgcggcgacc gtcgttttca
     9541 cgcagtaaac catcaacaat tttatgcatg taataaagcg agaactgttg ataatcgcgc
     9601 ccggtaagca cgccgcccca ggtgtcgaaa atcatcacag cctgagcacc ggctttaatc
     9661 tgcgcattca gatacaaagt gacgcttttc gccagtttat cgagtagagc gtgcagcgcc
     9721 tgcggatcgg catacatcat ttttttgatc acggtgaagg ctttgctgct gccgccttcc
     9781 accatgtagg tcgccagcgt ccacgggctg ccggaaaaac caatcagcgg gacgtcgcct
     9841 ttcagttcgc ggcgaatggt acgcaccgcg ttcatcacgt aacccagctc atcttccggg
     9901 tccggaattg gcagtttatc gacgtcagct ttgcaggtga ctggcgaggt aaaacgcgga
     9961 ccttctccgg cctcaaaata gagccctaac cccatcgcgt ccggcacggt gaggatatcg
    10021 gaaaagagga tcgccgcatc cagcgggtag cgacgcagcg gttgcaaagt cacttcgcac
    10081 gccagctcgg cgtttttgca cagcgacata aaatcgcctg cctgggcgcg cgtggcttta
    10141 tattccggta gatagcgacc cgcctggcgc atcatccata ctggagtgac atcaacgggc
    10201 tggcgcagca gcgcccgcag ataacgatcg tttttaagtt cggtcatttt ggctgttcct
    10261 tagtgcgtca ggcggtcagt gtatcatcac tcgtactctg cccgacacat cgccaccgta
    10321 tcttctatca gacggcgcgc cacggtgccg ggcggcggga gtaacggcaa atcgtcatag
    10381 cgataccagt tcgcctcgag caattctttc gggtcgatca cgatgtcgcc gctgtcatat
    10441 tccgccataa acgcggtcat taaagactga ggaaacggcc acggctggga agtcacataa
    10501 cgcaagtttt taactttaat tccgctctct tccatcactt cccgcgcgac cgcctgctcg
    10561 agggtttcgc ccacttcgac gaatccggca agtaccgtat ggacaccgtt acgatggcgg
    10621 gtatgctggg cgaggaggat cgaatcatcg cgacggatgg caacaataat gcagggggcg
    10681 atttgcgggt agtaacgctc acggcaatgg ctgcacagca tcgcccattc ggttttgctt
    10741 ggatacattt catgcccgca gtaaccacag tatttatgcg atcggtaaaa ctccgccagt
    10801 tgcacgccac gtccggccag ttgaaacagc ccaacatcga gatcaatgac ctgacgtacc
    10861 gaccccatat cgtgacgccg ctgctgttgt attaaccaaa caggttcccc ctgccattcg
    10921 ccgatttgta gtgcgcgctg acccacaaga tcgaaatttg ccgcttcgcc atatggcaat
    10981 tctcccttcg gcaaccataa tttttgttca tggctgacga cccagcagcc gtgatctaat
    11041 ttttcaatta tacgatccat agctcttgca ctacctttgc atcactggca tgtttaacat
    11101 ggtttttaca tttctcactg agcagttttt gaatacaaac ttgcggagtc aatcatgctt
    11161 aaccagctcg ataacctgac ggagcgcgtc agaggaagta acaaactggt tgatcgctgg
    11221 ctacatgtac gtaagcatct gctcgtggct tactacaatc tggttggcat taagcctggc
    11281 aaagaatcgt acatgaggct aaacgaaaaa gcccttgatg atttttgtca gagcctggtc
    11341 gattacttgt ctgccggaca tttcagtatt tatgagcgca ttcttcataa gctggaaggc
    11401 aacgggcaac tcgcgcgcgc cgcgaagatt tggccgcaac tcgaagccaa tacccaacag
    11461 attatggatt actacgattc cagcctggaa accgctatcg atcatgataa ttaccttgag
    11521 tttcaacagg ttttatctga catcggcgag gcgctggaag cgcgctttgt gctggaagat
    11581 aagctgattc tgctggtgct tgacgccgcc cgcgtcaaac atcctgcttg agttctgcgc
    11641 tgttaacgcg taatttacat tcaacgcccc gtttgtaggg ctaatttctt gtcggagtgc
    11701 cttaactggc tgagaccgtt tattcgggat ccgcggaacc tgatcaggct aatacctgcg
    11761 aagggaacaa gagttaatct gctatcgcat cgcctttgcg gcgatcgtct cttgcttcat
    11821 ccgtcgtctg acaagccacg tccttaactt tttggaatga gctatgtctg caacaaaact
    11881 gacccgccgc gaacaacgcg cccaggccca acattttatc gacaccctgg aaggcaccgc
    11941 ctttcccaac tcaaaacgca tttacatcac tggcgcacac cccggcgtgc gcgtgccgat
    12001 gcgtgagatc cagcttagcc cgacgctaat cggcggcagc aaagaacagc cgcagtacga
    12061 agaaaacgaa gcgattccgg tctacgacac ctccggcccg tatggcgatc cgcagatcgc
    12121 cattaacgtg caacaagggc tggcaaaact acgccagccg tggatcgatg cgcgcggcga
    12181 taccgaagaa cttaccgtgc gcagttccga ttacactaaa gcgcggctgg cagatgatgg
    12241 cctcgacgag ctgcgtttta gcggcgtatt aacgccaaaa cgcgccaaag caggacgccg
    12301 cgtcacacaa ctgcactacg cccgccaggg catcatcaca ccggaaatgg aattcatcgc
    12361 catccgcgag aatatgggcc gcgagcgcat ccgtagcgaa gttttacgcc accagcatcc
    12421 gggaatgagc tttggcgcac gtctgccgga aaatatcact gcggaatttg tccgtgatga
    12481 agttgctgcc ggacgtgcga ttatcccggc caacattaat catccggaat cggagccgat
    12541 gattattggc cgtaacttcc tggtaaaagt taacgccaat atcggcaact cagcggtcac
    12601 ctcttccatc gaagaagaag tggaaaagct ggtatggtcc acgcgctggg gagcggatac
    12661 ggtgatggat ctctccaccg gtcgctatat tcacgaaacc cgcgagtgga ttttgcgtaa
    12721 cagcccggtg ccgatcggta cagtgccgat ctaccaggcg ctggagaagg ttaacgggat
    12781 cgccgaagat cttacctggg aagcgttccg cgacacgctg ctggaacagg ccgagcaagg
    12841 tgtggattac ttcactatcc atgcgggggt gctgctgcgc tatgtgccga tgaccgcgaa
    12901 acgcctgacc ggtatcgtct cgcgcggcgg ttcgattatg gcgaaatggt gcctctccca
    12961 tcatcaggaa aatttcctct atcaacactt ccgcgaaatt tgtgaaatct gtgccgctta
    13021 tgacgtttcg ctgtcgctgg gcgacggtct gcgccccggt tctattcagg acgccaacga
    13081 tgaagcacag tttgccgagc tgcatacgct gggcgaactg accaaaattg cctgggaata
    13141 tgacgtgcag gtgatgattg aaggcccagg ccacgtgccg atgcagatga tccgccgcaa
    13201 tatgaccgag gagttagagc actgccacga agcgccgttt tacactctgg ggccgctaac
    13261 taccgatatt gcgccgggct atgaccactt cacgtcgggg attggtgcgg cgatgattgg
    13321 ctggtttggc tgcgcgatgc tctgttacgt aacgccaaaa gagcatctgg gtctgcctaa
    13381 taaagaagat gttaagcagg ggcttatcac ctataagatt gccgcccacg ccgctgacct
    13441 ggcgaaaggg catccgggcg cgcaaattcg cgataacgcc atgtcgaaag cccgcttcga
    13501 atttcgctgg gaagaccagt ttaatctggc cctcgacccg tttaccgccc gcgcttatca
    13561 cgatgaaacc ctgccccaag agtccggcaa agtcgcccat ttttgctcca tgtgtggacc
    13621 gaaattttgc tcgatgaaaa tcagccagga agtgcgtgat tacgccgcca cgcaaactat
    13681 tgaaatggga atggcggata tgtcggagaa cttccgtgcc agaggcggag aaatctacct
    13741 gcgtaaggag gaagcgtgat gtatcagcct gattttcctc ctgtaccttt tcgtttagga
    13801 ctgtacccgg tggtggacag cgtacagtgg atcgaacgtc tgttggatgc aggcgtacgt
    13861 actctccagc tacgcatcaa agatcggcgc gatgaagagg tggaagccga tgtcgtggcg
    13921 gcaattgcgc tgggccgccg ctataacgcg cgattgttta tcaacgatta ctggcggctg
    13981 gcgatcaagc atcaggcgta tggcgtccat ttggggcagg aagatttgca agccaccgat
    14041 ctcaatgcca tccgcgcggc aggcctgcgg ctgggcgttt cgacacatga cgatatggaa
    14101 atcgacgtcg cgctggcagc acgcccctct tatatcgcgc tgggacatgt gttcccgacg
    14161 caaaccaaac agatgccttc tgcaccgcag gggctggaac agctggcacg gcatgttgag
    14221 cgcctggcgg attatcccac cgtggcgatt ggcggtatca gtctggcgcg tgcgcctgcg
    14281 gtgatagcaa cgggtgtcgg cagtatcgcc gtcgtcagcg ccattactca aaccgcagac
    14341 tggcgtttgg caacggcaca gttgctggaa attgcaggag ttggcgatga atgaccgtga
    14401 ttttatgcgt tatagccgcc aaatcctgct cgacgatatc gctcttgacg ggcagcaaaa
    14461 actgctcgac agccaggtgc tgattatcgg tcttggcggg ctgggtacac ctgctgcgct
    14521 atacctggcg ggcgctggcg tcgggacgct ggtactggca gatgacgacg atgtgcattt
    14581 aagcaatctg caacgacaaa tcctctttac cactgaagat atcgatcgcc cgaaatcgca
    14641 ggtcagccat cagcgactga cacagttgaa tcccgatatt caactgacag cattacaaca
    14701 acggttaacg ggtgaggcgt taaaagatgc ggttgcacaa gccgatgtgg tgctcgactg
    14761 taccgacaat atggcgactc gccaggagat taatgccgcc tgcgtggcac tcaacacgcc
    14821 gcttatcacc gccagcgcgg tcggatttgg cggtcagttg atggtactga cgccgcccta
    14881 ggagcagggg tgttaccgct gcctgtggcc agataaccag gagccagaac gcaactgccg
    14941 cacggcgggc gtggttggcc cggtggtcgg ggttatgggc actttgcagg cactggaagc
    15001 cattaagtta ttaagcggta tagagacgcc tgcgggagaa ctccgactgt tcgacggtaa
    15061 gtcgagccag tggcgcagcc tggcgttgcg ccgcgccagt ggttgcccgg tatgcggagg
    15121 aagcaatgca gatcctgttt aacgatcaac cgatgcagtg cgccgccggg caaactgttc
    15181 acgaactact ggagcaactc gaccaacgac aagcgggcgc ggctctggcg attaatcatc
    15241 aaatcgtccc gcgtgagcag tggacgcaac atatcgtgca ggatggcgac cagatcctgc
    15301 tttttcaggt tattgcaggg ggttgagatg ttacgtattg cggacaaaac gtttgattca
    15361 catctgttta ccggcaccgg gaaattcgct tcttcacaac tgatggtgga ggcgatccgc
    15421 gcttccggca gccagctggt gacactggcg atgaaacgtg tcgacttgcg ccagcacaac
    15481 gacgctatcc tcgaaccgct tatcgcggcg ggtgtgaccc tgctgccaaa tacatccggg
    15541 gcgaaaacag cggaagaagc cattttcgcc gcccatctgg ctcgtgaagc gttaggcaca
    15601 aactggttaa aattagagat tcaccctgac gcccgctggc tgttgcccga tcccatcgaa
    15661 accctgaaag ccgccgaaac gctggtacaa cagggatttg tcgtgctgcc ttactgcggg
    15721 gccgatccgg tattgtgtaa acgtctggaa gaagtcggct gtgcagcggt gatgccgctc
    15781 ggcgcgccga ttggctcgaa tcaggggctg gaaacccgcg ccatgctgga gattattatc
    15841 cagcaggcca cagttccggt ggttgtcgat gctggcatcg gcgttcccag ccatgccgcg
    15901 caggcgctgg aaatgggggc cgacgcggtg ttagtgaata cggcgattgc cgtcgcggac
    15961 gatcccgtca acatggcgaa ggcatttcgt ctggcagtag aagcaggcct gctggcacgt
    16021 cagtccggac cgggcagccg cagtcatttt gctcatgcca ccagcccgct gaccggattt
    16081 ctggaggcat cggcatgaaa accttcagca atcgctggcg acaactggac tgggacgaca
    16141 tccgcctgcg tatcaacggc aaaacggctg ctgacgtaga gcgggcgcta aatgcctcgc
    16201 aactcacccg cgacgacatg atggcgctgt tatcgcctgc cgccagtggc tatctggaac
    16261 aactggccca acgggcacag cgtctgaccc gtcagcgatt tggcaacaca gttagtttct
    16321 acgtcccgct ttatctttcc aatctttgcg ctaacgactg cacgtactgc ggattttcca
    16381 tgagtaatcg catcaagcgc aaaacgctgg atgaagcgga tattgccagg gaaagcgccg
    16441 ctatacggga gatgggcttt gaacatctgc tattagtcac tggtgaacat caggcgaaag
    16501 tggggatgga ttactttcgt cgtcatctcc ctgccctgcg tgaacagttc tcttcactac
    16561 agatggaagt gcaaccgctg gcggagacgg aatacgccga gttaaagcaa cttggtctgg
    16621 atggggtgat ggtttatcag gagacatatc acgaggcgac atatgcccgc catcatctga
    16681 aaggcaaaaa acaggacttc ttctggcggc tggaaacgcc ggatcggctg ggccgtgcgg
    16741 ggattgataa gataggcctc ggcgcgctaa ttggcctttc cgacagctgg cgcgttgact
    16801 gctatatggt tgcagaacat ttgctatggc tgcaacagca ttactggcaa agccgttact
    16861 ctgtctcctt tccgcgcctg cgcccgtgta ctggcggcat tgagcctgcg tcgattatgg
    16921 atgaacgcca gttagtgcaa accatctgcg ccttccgact gcttgcaccg gagattgaac
    16981 tgtcactctc cacgcgggaa tcaccgtggt ttcgcgatcg cgttattccg ctggcgatca
    17041 ataacgtcag cgccttctcg aaaacgcagc caggtggcta tgctgataat caccccgagt
    17101 tggaacagtt ctcaccgcac gacgatcgca gaccggaagc ggttgctgca gcgttaaccg
    17161 ctcagggttt gcagccggta tggaaagact gggacagcta tctgggacgc gcctcgcaaa
    17221 gactatgaca cggaatgtaa gaacgtaaat ttattccgag ccgccacgga agatgaaaaa
    17281 cgcaaggttg ttgaaagcgt tgcgtttttt tatggtagtg ccgtcagcag gaatgttgtc
    17341 cagggcgaaa agcttcttcc tcgtttcgcc ctgcctttct taaaaattcc gtaatgcaaa
    17401 gtctgaagcc atcgcctgtt gagcaatgaa agagatctga ttgtaagaga gtaaatactc
    17461 aactatgata gagacgaaaa taagaacaca tgttctcatc ttccaggatg cagcagactg
    17521 aagaaattca gacatcccgc aacctgcgat tatcgcaagg tcaaggcaaa gtccggtaat
    17581 ggcgttctga ataccagaga taattctctg gcgaaaccca ccttaaggtg ggttttgtta
    17641 ttttgagggc tgaggaagct gcttatttct caataagttg taggcaatgg tgaaaaattt
    17701 cactgattta cctttctgtt tttcagaaat gggataagcg ctagttacat gcaaacgaag
    17761 ttttttcttt tccctgaaag ccctgaacgc aacaaagtaa aatgccttgt ctcctccgtc
    17821 taagtccacc tcaatcacgg catagctacc atacccggca tgaataacgt tgctttctgg
    17881 cagcgccaaa atagttcttt ttaagtgtgt gcgcgctaag ttataacggt gctggcagaa
    17941 gggacgagat tctttaggcg cgtggtacat taacgattgt ttttcttcgt tcgtctgatg
    18001 ttcataatct tttgtgaagc agtgaagaga gtaggtaacc caaaagcgat actctaaagg
    18061 ctctttgccc tcatgatgct gaacgtagac agcctgatga gcatcaagaa atgataaatc
    18121 acgatatcac catccggatg tccaaaaggt cgccactttt ccacttcctg tttcattctc
    18181 gttccttaag aaagttacac aggaatagta tcgaatcctt gccgagtttg actcaattag
    18241 ttcagtcagt ttcaggatat tagtcatctc tacattgatt atgagtatcc agaaattcat
    18301 taaatattct gacaaatgct ctttccctaa actcccccca taaaaaaacc cgccgaagcg
    18361 ggtttttacg ttatttgcgg attaacgatt actcgttatc agaaccgccc agacctgcgt
    18421 tcagcagttc tgccaggctg gcagatgcgt cttctgcagt cacctgcggt gcagccggag
    18481 cttcacccgc agcacggcga cgcatacgat cctggtggta cgcgtaaccg gtacctgccg
    18541 ggatcagacg acccacgata acgttctctt tcaggccgcg cagttcgtcg cgtttgcccg
    18601 caacggctgc ttcggtcagc acgcgagtgg tctcctggaa cgatgccgcg gagatgaagg
    18661 actcggttgc cagagacgct ttggtgatac ccagcagatc gcgggagtaa gttgcaccca
    18721 ctttgccgtt cgcttccagt tcgcggtttg cgatcttgac gcgagagtat tcaacctgtt
    18781 cgccttccag gaagtcggag ctgcccgcat taacgatggt agctttacgc agcatctgac
    18841 gaacgataac ttcgatgtgt ttatcgttaa tcttaacgcc ctgcagacgg tatacgtcct
    18901 gtacttcgtt aacgatgtaa cgagtcacag catgaacacc acgcagacgc agaatgtcgt
    18961 gcggcgcttc cggaccgtcg gaaattacgt caccacgttc tacacgttca ccttcgaaca
    19021 cgttgagctg acgccatttc ggaatcatct cttcgtacgg atcgctaccg tctaccgggg
    19081 tgataaccag acgacgttta cctttggttt ctttaccgaa ggaaacgata ccgctgattt
    19141 cagccaggat tgccggctct ttcggacgac gtgcttcgaa caggtccgca acgcgtggca
    19201 gaccaccggt gatgtccttg gtaccgccgg attcctgcgg aatacgcgcc agggtgtcac
    19261 cagagctgat ctgtacgcca tcttccagct gaacaatcgc tttacccggc aggaagtact
    19321 gcgcaggcat atcggtacct gggatcagaa cgtcgttacc ctgagcatca acgattttca
    19381 gtgccggacg cagatcttta ccacctgcgg tacgttctgc ggaatccaga accaccagcg
    19441 aagacagacc ggtcagttcg tcggtctgac gagtaatggt ctggccgtcg atcatgtcag
    19501 taaagcgtac aaaaccgctt acttcggtga taaccggcat ggtgtgcggg tcccagtttg
    19561 caacggtttc gccgccagca acctgttcgc catcgccttt cgccagtacc gcaccgtaag
    19621 gtactttgta gctttcttta gtacgaccga attcgtcgat cagtttcagt tcggtgttac
    19681 gggaagtgat aaccagttta ccgctggagt tcacaaccga cttcacgttg ctgagcttga
    19741 tgctaccttt gtttttcacc tggatgctgg attcagcagc cgcacgagat gccgcaccac
    19801 cgatgtggaa cgtacgcatg gtcagctgtg tacccggttc accgatggac tgtgccgcga
    19861 taacaccgat tgcttcaccc ttgttgatga tgtggccacg cgccaggtca cgaccgtagc
    19921 agtgcgcaca tacaccaaag tcggtgtcac aagatacaac agaacgtact ttaaccgcgt
    19981 cgacagagtt ctcttccagc aggtcacacc actgttcgtg cagcagcgtg ttgcgcggaa
    20041 cgaggatatc agcagtaccc ggcttcagaa cgtcttcagc agttacacga cccagtacgc
    20101 gatcgcgcag cggctcttta acgtcaccac cctcgataac cggagtcatc atgatacctt
    20161 catgggtacc acaatcgtct tcggtaacca ccaggtcctg cgccacgtca accagacgac
    20221 gagtcaggta accggagttc gcagttttca gtgcggtatc cgccagacct ttacgagcac
    20281 cgtgggtgga gatgaagtac tggagtacgt tcagaccttc acggaagttc gcggtgattg
    20341 gcgtttcgat gatggagcca tccggcttcg ccatcagacc acgcatacca gcaagctgac
    20401 gaatctgtgc cgcagaacca cgcgcaccgg agtcggccat catgtagatg ctgttgaagg
    20461 aaacctgctt ctcttcctga ccgtcacggt taataacggt ttcagtttgc aggttatcca
    20521 tcatcgcttt ggatacacga tcgttcgccg cagcccagat atcgataact ttgttgtagc
    20581 gttcgcccgc agttaccaga ccagactgga actgctcctg aatttcagca acttctgctt
    20641 ctgcctcgga gatgatttcg tgtttcttct ccgggatgac catgtcatcg ataccaacag
    20701 atgcaccaga acgcgctgca tatgcaaagc cggtgtacat gatctggtcc gcaaaaataa
    20761 cggtcggttt cagaccgaga atgcggtagc aggtgttcag cattttggag attgcttttt
    20821 tacccagcgc ctggttgacg atggagtaag gcagaccttt cggtacaatc atccacagaa
    20881 tggcacggcc aacagtcgtg tctttcaggc tggttttcgc tactaattca ccgttagcat
    20941 ctttttcata ctcggtgata cgcactttaa cgcgcgcatg cagagaagcc agaccagagc
    21001 gatacagacg ttctgcttct ttcgggccag tcagcaccat gccttcgcct ttggcgttaa
    21061 cacagtcacg ggtcatgtag tacagaccca gtacaacgtc ctgagacgga acgatgattg
    21121 gttcgccgtt cgccggggac aggatgttgt tggtagacat catcagcgca cgcgcttcca
    21181 gctgggcttc cagcgtcagc ggtacgtgaa cagccatctg gtcaccatcg aagtcggcgt
    21241 tatatgccgc acaaaccagc gggtgcagct ggatagcttt accttcgatc agtaccggtt
    21301 caaatgcctg gatacccaga cggtgcagag tcggtgcacg gttcagcagt accgggtgtt
    21361 cgcggataac ttcgtccagg atatcccaaa cgacagcttc ttcgcgctca accattttct
    21421 tcgcagcttt aatggtggta gcaagaccac gcagttcaag cttgccgtag atgaacggtt
    21481 tgaacagctc cagcgccatt ttcttcggca gaccgcactg atgcagacgc aggtatggac
    21541 ctacggtgat tacagaacga ccggagtagt caacacgctt accgagcagg ttctgacgga
    21601 aacgaccctg tttacctttg atcatgtcgg ccaaagattt cagaggacgc ttgttagaac
    21661 cggtgatcgc acgaccgcga cgaccgttat ccagcagggc gtctaccgct tcctgcagca
    21721 tacgtttttc gttacgtacg atgatgtccg gcgcagccag atccagcaga cgtttcagac
    21781 ggttgttacg gttaatgacg cgacgataca gatcgttcag gtcagaagtc gcgaaacgac
    21841 caccatccag cggaaccagc ggacgcagat ctggcggcag taccggcaga acggtcagga
    21901 tcatccactc tggtttgtta ccagactgaa cgaacgcttc cagcagtttg atacgcttgg
    21961 tcagcttttt acgcttagtt tcggagttgg tttcgttcag ctcttcacgc agctgttcgc
    22021 actcttgctc cagatccatg ctcttcagca gagcctggat tgcttccgca cccattttcg
    22081 cgtcgaattc gtcaccgaac tcttccagcg cgtccagata ctgctcttca gtcaggatct
    22141 gctgacgttc caggttggtc ataccgcctt cgataaccac ataggattcg aagtacagta
    22201 cgcgttcaat atcgcgcagc ggcatatcga gcagcaggcc gatacgggac ggcagcgatt
    22261 tcaggaacca gatgtgcgca gtcggggaag ccagttcgat gtggcccata cgctcacggc
    22321 gtactttagt ctgggtcact tcaacgccac acttctcaca gatgacgcca cggtgtttca
    22381 ggcgcttgta cttaccgcac aggcactcgt aatcttttac cggcccaaag atacgggcgc
    22441 agaaaaggcc gtcacgttct ggtttgaacg tacggtagtt gatggtttcc ggctttttaa
    22501 cttcaccgaa agaccatgaa cggatcatgt ctggcgaagc cagagcaatt ttgatcgcat
    22561 caaactcttc ggttttagtc tgcgctttca gaaactttaa taaatctttc acggatttgc
    22621 tcccgtcgga gttagcacaa tccgctgccg ggttttaccc cgacagcagt gacctgtttg
    22681 agcgagaatt actcgtcttc cagttcgatg ttgataccca gcgaacgaat ctctttcaac
    22741 aatacgttga aggattctgg catgcccggc tccatctgat ggttgccgtc cacgatgttt
    22801 ttatacatct tagtacgacc attcacgtca tcagacttaa cggtgagcat ttcctgcagg
    22861 gtgtatgctg cgccgtatgc ttccagcgcc cacacttcca tctccccgaa acgctgacca
    22921 ccgaactgtg ccttaccacc cagcggctgc tgagtaacca ggctgtaaga accggtggaa
    22981 cgcgcgtgca tcttgtcgtc gaccaggtgg ttcagtttca gcatgtacat gtaaccaacg
    23041 gttaccggac gctcgaactg ttcaccagtg cgaccatcgt acaggcggat ctgaccggaa
    23101 gtcggcaggt cgccaagttt cagcagctct ttaatttctg cttctttcgc accgtcgaac
    23161 accggcgttg cgattggcat acctttgcgc aggttttcag ccagacgcat aacttcttca
    23221 tcgctgaagg tactcaggtc aactttctga cgaacgtcag cgcccagatc gtacgcacgc
    23281 tggatgaatt cgcgcagttt cgcgacttct tgctgctgtt tcagcatggc gttgatcttg
    23341 tcgccgatac ctttcgcagc catacccagg tgggtttcga ggatctgacc gatgttcata
    23401 cgagacggta cgcccagcgg gttcagtacg atgtctaccg gcgtaccgtt ttcatcgtaa
    23461 ggcatatctt cgatcgggtt gatcttagaa attacaccct tgttaccgtg acgacctgcc
    23521 atcttgtcac caggctggat acggcgttta accgccagat ataccttaac aatcttcagc
    23581 acgcccggtg ccagatcgtc gccctgggtg attttgcggc gtttcgcttc gagtttcttc
    23641 tcgaactcgt gtttcagttc gtcatactgc tcagccagct gttccagctg attttgtttc
    23701 tcttcgtcgg tcaggcccag ctccagccag cgatcgcgcg gcagtttgtc gagcttctca
    23761 gcttcaacgc caccggctac cagcacagca cggatacggc tgaacagacc cgcttcgagg
    23821 atctgcagtt cttcagacag gtctttcttc gcctgtttga gctgcatttc ttcgatttcc
    23881 agcgcacgtt tgtctttttc tacgccatcg cgagtaaaga cctgaacgtc gataaccgta
    23941 ccggatacac cgtttggtac gcgcagagaa gagtctttaa cgtcagacgc tttctcaccg
    24001 aagatcgcac gcagcagttt ttcttctggg gtcagctgag tttcaccttt cggcgtaacc
    24061 ttaccaacca gaatgtcgcc accggtcact tccgcaccaa tataaacgat accggattca
    24121 tccagtttgg agagcgcagc ttcacccacg ttcgggatgt cagcggtgat ctcttccggc
    24181 cccagcttgg tgtcacggga cacacacgcc agttcctgaa tgtggatggt ggtgaaacgg
    24241 tcttcctgaa caacacgctc ggatacgagg atggagtctt cgaagttgta accattccac
    24301 ggcatgaacg ctacgcgcat gttctgacca agcgccagtt caccgaggtc ggtggacgga
    24361 ccgtctgcca gcacgtcgcc acgttcaacc ggttcaccca gagacacaca cggcatctgg
    24421 ttgatacagg tgttctggtt agaacgggtg tatttggtca ggttgtagat gtcgatacct
    24481 gcttcacccg gatacatctc gtcttcgtta actttgataa cgatacggga agcatccacg
    24541 tactgaacga caccaccacg tttagcaacc gcagttacac cggagtcaac ggcaacagca
    24601 cgttccatac cagtaccaac cagcggctta tcagcacgca gagtcggaac ggcctgacgt
    24661 tgcatgttcg cacccatcaa tgcacggttg gcgtcatcgt gttccaggaa cgggatcagg
    24721 gacgcaccga cggataccac ctgctgggtg gatacgtcca tgtagtcaac ctggtcacgg
    24781 ctgaacaagc tggattcgcc tttgctacgg caggttacca ggtcttctac gaagtggcct
    24841 tcttcatcca ggttggagtt cgcctgggcg ataacgtagt tgccttcttc gatagcagac
    24901 aggtagtgaa tttcgtcagt tacaacaccg tcggtcactt tacgatacgg agtctcaagg
    24961 aagccgtatt cgttagtctg tgcgtacacg gacagagagt tgatcagacc gatgttcgga
    25021 ccttcagggg tttcgattgg acatacgcga ccgtagtgag tcgggtgtac gtctcgaact
    25081 tcgaagcctg cacgttcacg ggtcagaccg cctgggccga gtgcggagat acgacgtttg
    25141 tgcgtaatct cagacagcgg gttgttctgg tccataaact gagacagctg gctggaaccg
    25201 aagaactctt tcactgctgc ggaaatcggc ttggcgttga tcatatcctg aggcatcagg
    25261 gtatccagat cgcccagaga cagacgctct ttcaccgcac gctctacacg taccaggcca
    25321 acgcggaact ggttttccgc catttcgcca acggaacgga tacgacggtt gccgaggtgg
    25381 tcgatatcat cgacttcgcc tttaccgtta cggatatcga tgagcttttt cataacatca
    25441 atgatgtcgt ctttgctcag gataccagaa ccttcgattt cttcgcgcag cagagaacgg
    25501 ttgaacttca tacgaccaac cgcagacagg tcataacggt cttcggagaa gaacaggttc
    25561 tcgaacaggc tttccgctgc ttcacgagtc ggcggctcgc cagggcgcat catgcggtag
    25621 atttctacca gtgcgctcag acggtcgtta gttgggtcga cacgtaaggt ttcagagata
    25681 tatgggccgt gatccagatc attggtgaac agcgtttcga tacgcttgtg accagactgg
    25741 ctcagcttag ccagcagatc caggctcagc tccatgttcg ctgcgcagat cagctcgccg
    25801 gtagactcat caatatagtc tttagcaacc actttacctg cgatgtactc aaccgggact
    25861 tcgatcagtt tgacgtcgtc tttttccagc tggcgaatgt ggcgcgcagt gatacggcgg
    25921 cctttttcta cgtacacttt accgttagct tcgatgtcga aagatgcggt ttcaccacgc
    25981 aggcgttccg gcaccagttc catctgcagc ttgttatcac ggatttcaaa gataactttt
    26041 tcaaagaaca ggtcgaggat ctgctctgtg gtgtagttca gggcacgcag aatgatggtc
    26101 gcaggcagtt tacggcgacg gtcgatacgt acgaacaggt tgtccttcgg atcgaattcg
    26161 aagtccagcc aggaaccacg gtaagggatg atgcgcgcgt tatacagcac tttacccgaa
    26221 gagtgggttt tacctttgtc ggagtcaaag aagacgcccg gactacggtg cagctgggaa
    26281 acgataacac gctcagtacc gttgataaca aaggtaccgt tgtctgtcat gagcggaatt
    26341 tcgcccatgt agacttcttg ttctttaatg tcttttacgg tgccttccgg cgcttcgcgc
    26401 tcatagatca ccagacgcag tttaacgcgc agcggtgcgg aataggtcac gccacggatt
    26461 tgacattcct ggacgtcaaa caccggttcg ccaaggcggt agctgacgta ttgcagctcg
    26521 gaattaccgc tgtagctctg aatcgggaat acggaacgga aagcagcttc cagaccatac
    26581 tgcccttcag gatcttgctc gataaatttc tgaaacgagt caagctggat agaaaggaga
    26641 taaggtacat ccagaacttg tggacgttta ccaaaatcct tacgaatacg ttttttctcg
    26701 gtataggagt aaaccatagg gttcctcagc tcgctgacaa gtcgacccat ctgtccattg
    26761 agcggacagt ttgtgcaaca ctattttgtt gaccggaaaa tggaacactt tccgcaatgc
    26821 ctgttgctat cacgcttaaa ccatttcatt gcgatttaca cagaacggac gtcctgtcgc
    26881 agtatattaa gtcgtcgata gaaacaagca ttgaaaggca cagcagtagt caaacagtgt
    26941 gaaacgctac tggcgcctta cagcgcaaaa aggctggtga ctaaaaagtc accagccatc
    27001 agcctgattt ctcaggctgc aaccggaagg gttggcttat ttaacttcaa cttcagcgcc
    27061 agcttcttcc agagcttttt tcagtgcttc tgcgtcgtct ttgctcacgc cttctttcag
    27121 agcagccggt gcagattcta ccaggtcttt agcttctttc agacccaggc cagttgcgcc
    27181 acgtactgct ttgataacag caactttgtt agcgccagca gctttcagaa ttacgtcgaa
    27241 ttcagttttt tcttcagcag cttcaaccgg gccagcagct acagctacag cagcagcagc
    27301 ggaaacaccg aatttttctt ccattgcaga gatcagttct acaacgtcca ttacagacat
    27361 agctgcaact gcttcaatga tttgatcttt agtgatagac atttaaattg ttcctgaata
    27421 tcagaataag tttatacgta agcgaatgcg ttaaaaagat aactgcgatt aagcagcttc
    27481 tttcgcatcg cgtacagcag ccagagtacg aaccagtttg ccagccgaag cttctttcat
    27541 ggttgccatc aggcgtgcaa ttgcttcttc gtaggtcggc agagttgcca ggcggtcgat
    27601 ctgagacgcc gggatcagct caccttcaaa ggcagcggct ttgacctcaa attttgcatt
    27661 cgctttcgcg aactctttga acagacgagc agcagcgccc gggtgttcca tagagtatgc
    27721 aatcagggtc ggaccaacaa acgcgtcttt caggcactcg aacggagtac cttcaacagc
    27781 gcggcgcagc agggtgttac gaacaacacg catgtatacg ccagcttcgc gacctgcttt
    27841 acgcagttca gtcattttat ctacagttac gccacgggaa tccgcaacta ctgcagacag
    27901 cgcgcctttg gctacttcgc tgacttcagc aacaatcgct tgtttgtctt gaagatttaa
    27961 agccattagc tttgctcctg gatgtttgcc agagaaaatc tctggaactc acttcactcc
    28021 tccaaacgga gagagcgtct taattacggt gagcagaaac aagccagaat ataaaagaat
    28081 aatcttagcg ttctgtcacc gtctacgcag gggattaagt ttcttgtgaa actcctgcgg
    28141 tcttcgacgg aggcctggat aggccaggct ccaacgaaca aatcttttta gctgctaact
    28201 ttcgttagca tacgtggggg taagattgta gacaaaatca ccgcccacgt aaaggcatta
    28261 gtttacagaa gcgctcaggc cagcctggtc aactgcaaca cctgcaccca tggtggtgga
    28321 gatgctaact ttcttgatgt acacgccttt cgcctgagtc ggttttgctt ttttcagcgc
    28381 aaccagcaga gcttccaggt tttctttcag tttgtcagcg tcaaagtcca ctttaccgat
    28441 ggtggtgtgg atgatgccgt ttttgtcgtt acggtaacga acctggccag ctttagcgtt
    28501 tttaaccgct tcagcaacgt tcggcgttac agtacccact ttcgggtttg gcatcaggcc
    28561 gcgcggaccc agaacctggc ccagctggcc aacaacgcgc attgcatccg gagaagcaat
    28621 aacaacgtca aagttcattt cgcctttctt gatctggtca gccagatctt ccatacctac
    28681 cagttcagca cctgcagctt tagcagcttc agcgtttgca ccctgggtaa atacggctac
    28741 gcgaacggaa cggccagtac cgtgcggcag tacagttgca ccacgtacgt tctggtcaga
    28801 tttacgagcg tcgatgccga ggttaacagc aacgtccacg ctttctacga atttagcagt
    28861 cgccagctct ttcagcagag cgatagcttc gttgatgtcg tactgtttag ttgcatcaac
    28921 tttctcgcgg ataacacgca tgcgcttggt cagtttagcc atttcttagt cctccactac
    28981 caggcccatg gaacgtgcag taccttcgat ggagcgagtc atcgcttcaa tgtcggcacc
    29041 agtcatgtcg gcagctttgg tctgcgcgat ttcctgcagc tgagcgcggg aaattttacc
    29101 cactttgtct ttgttcggct taccggaacc agacttgata ccagccgctt ttttcagcag
    29161 aactgctgcc ggcggagtct tggtaacgaa agtgaaagaa cggtcagcgt aaacggtgat
    29221 tactaccgga atcggcagac ctttttcgat ggaatcagtt tttgcgttga acgctttgca
    29281 gaattccatg atgtttacgc cctgctgacc cagagccgga cctactggcg gactcgggtt
    29341 agccatacca gctgcaacct gcagcttgac ataggcttgt actttcttag ccattataaa
    29401 ttcctcaagt tgggtaataa cgcctctgag aggctccccg tgatataaaa aatcgtttta
    29461 cgggcaaggc ccataaaaac aaaaggcgcg aaattgtatt ccaatctcgc gccttgtgca
    29521 acgattaaat cgccgctttt ttgatcgctg ggttaggctt tttcaacctg gctgaagtcc
    29581 agctctaccg gggtcgcacg accgaagata gaaacagaca ctttcagacg agatttctcg
    29641 taatccactt cttcaacaac accgttgaag tcagcgaacg gaccatcatt aacacggacc
    29701 atttcacccg gttcaaacag cgttttcgga cgcggcttat caccaacctg ctgcaggcgg
    29761 ttcataatcg catcgacttc tttatcgctg attggcgcag gacgatcgga agtaccgccg
    29821 atgaagccca tcacacgcgg tacgctgcgc accaggtgcc agctcgcgtc gttcatcacc
    29881 atctgaacga ggacgtagcc agggaagaat ttacgttcgc ttttgcgacg ctgaccgcca
    29941 cggatttcaa ccacttcttc ggttggtacc atgacttcac caaacaaatc ttccatgttg
    30001 tgtaatttga tatgctcacg cagcgacgtt gctacgcggc cttcaaaacc ggaaaacgcc
    30061 tgaacgacgt accagcgctt tttaggagct tcagacatct cagaacctca ggccagtgat
    30121 aaaggatacc aggcgaacca gaataccatc cagtccccac aggatcagtg acattactgc
    30181 ggtaaccgca gccacaatca gcgtggtgtg caatgtttcc tggcgagtcg gccaaatgac
    30241 cttacggact tcggtacgcg cttcacgggc aaaagcaacg gtagctttac cttttgtcgt
    30301 taacagcgcg acaccacccg ctgcagcaat cagaattact acggccagcg cacgcagcgg
    30361 cagcataatg tcgcgataaa ggtagttgcc gacaatcgcc accaggagca atgccaccac
    30421 aacgacccac ttcatcgctt ccaggccgcg cccgcttcct tgagcttcgg tattcgcact
    30481 cataaaccaa cctgtcagaa gtattctaca aacattttca ccccgcgatc gcgaggcaaa
    30541 ccaaatcgaa acgcgtatta gcttttcgga ttatacgccc tcaacagagc ctgtctcagc
    30601 aatgattatg acaaagaaaa tcactgatga gccaggttct ggtacgaaag cgtgcaaaaa
    30661 gggcatcaaa tgatgccctt ttagtgcgca ttgcgtcaaa tgttatcggc aattagctca
    30721 gaacttttgc tacaacgccc gcgccaacgg tacggccgcc ttcacggatt gcgaaacgca
    30781 gaccgtcgtc catcgcgatc gggtggatca gggtaacaac cattttgatg ttgtcgcccg
    30841 gcattaccat ctctacgcct tccggcagtt cgatggtacc agtcacgtca gtagtacgga
    30901 agtagaactg cggacggtag cctttgaaga acggagtatg acggccgcct tcatctttgg
    30961 acagaatgta cacttcagat tcgaacttgg tgtgcggctt gatggtgccc ggcttagcca
    31021 gtacctgacc acgttcgatt tcttcacgtt tgataccacg cagcagaaca cctacgttct
    31081 caccagcacg gccttcgtcc agcagtttgc ggaacatttc aacgccagta caggtagact
    31141 tctgagtctc tttgatacca acgatttcaa cttcttcacc aactttgatg ataccgcgtt
    31201 ctacacgacc ggtaacaacg gtaccacgac cggagatgga gaatacgtct tcgatcgg
//
LOCUS       DAZKFU020000029        30903 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZKFU020000029 DAZKFU020000000
VERSION     DAZKFU020000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.029"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(228..1169)
                     /gene="fabY"
                     /locus_tag="ACHOQQ_RS05335"
                     /old_locus_tag="pgaptmp_001067"
                     /gene_synonym="yiiD"
     CDS             complement(228..1169)
                     /gene="fabY"
                     /locus_tag="ACHOQQ_RS05335"
                     /old_locus_tag="pgaptmp_001067"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGU4469346.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1214..1651)
                     /gene="dtd"
                     /locus_tag="ACHOQQ_RS05340"
                     /old_locus_tag="pgaptmp_001068"
     CDS             complement(1214..1651)
                     /gene="dtd"
                     /locus_tag="ACHOQQ_RS05340"
                     /old_locus_tag="pgaptmp_001068"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGU4469347.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1648..2520)
                     /gene="yihY"
                     /locus_tag="ACHOQQ_RS05345"
                     /old_locus_tag="pgaptmp_001069"
     CDS             complement(1648..2520)
                     /gene="yihY"
                     /locus_tag="ACHOQQ_RS05345"
                     /old_locus_tag="pgaptmp_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGU4469348.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2514..3113)
                     /gene="yihX"
                     /locus_tag="ACHOQQ_RS05350"
                     /old_locus_tag="pgaptmp_001070"
     CDS             complement(2514..3113)
                     /gene="yihX"
                     /locus_tag="ACHOQQ_RS05350"
                     /old_locus_tag="pgaptmp_001070"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGU4469349.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TAFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3254..4141)
                     /locus_tag="ACHOQQ_RS05355"
                     /old_locus_tag="pgaptmp_001071"
     CDS             complement(3254..4141)
                     /locus_tag="ACHOQQ_RS05355"
                     /old_locus_tag="pgaptmp_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU4469350.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4131..5480)
                     /locus_tag="ACHOQQ_RS05360"
                     /old_locus_tag="pgaptmp_001072"
     CDS             complement(4131..5480)
                     /locus_tag="ACHOQQ_RS05360"
                     /old_locus_tag="pgaptmp_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4469351.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(5579..7012)
                     /locus_tag="ACHOQQ_RS05365"
                     /old_locus_tag="pgaptmp_001073"
     CDS             complement(5579..7012)
                     /locus_tag="ACHOQQ_RS05365"
                     /old_locus_tag="pgaptmp_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4469352.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNTLCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLIHWEPLGANVDAEDAEVLAELKK"
     gene            complement(7082..8092)
                     /locus_tag="ACHOQQ_RS05370"
                     /old_locus_tag="pgaptmp_001074"
     CDS             complement(7082..8092)
                     /locus_tag="ACHOQQ_RS05370"
                     /old_locus_tag="pgaptmp_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HGU4469353.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(8125..9012)
                     /locus_tag="ACHOQQ_RS05375"
                     /old_locus_tag="pgaptmp_001075"
     CDS             complement(8125..9012)
                     /locus_tag="ACHOQQ_RS05375"
                     /old_locus_tag="pgaptmp_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HGU4469354.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQIMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(9037..9915)
                     /gene="yihU"
                     /locus_tag="ACHOQQ_RS05380"
                     /old_locus_tag="pgaptmp_001076"
     CDS             complement(9037..9915)
                     /gene="yihU"
                     /locus_tag="ACHOQQ_RS05380"
                     /old_locus_tag="pgaptmp_001076"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGU4469355.1"
                     /translation="MATIAFLGLGQMGSPMANNLLHKGHSLQVFDVNALAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            10088..10984
                     /locus_tag="ACHOQQ_RS05385"
                     /old_locus_tag="pgaptmp_001077"
     CDS             10088..10984
                     /locus_tag="ACHOQQ_RS05385"
                     /old_locus_tag="pgaptmp_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4469356.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIARLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            11024..11812
                     /locus_tag="ACHOQQ_RS05390"
                     /old_locus_tag="pgaptmp_001078"
     CDS             11024..11812
                     /locus_tag="ACHOQQ_RS05390"
                     /old_locus_tag="pgaptmp_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4469357.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(11923..13188)
                     /gene="yihN"
                     /locus_tag="ACHOQQ_RS05395"
                     /old_locus_tag="pgaptmp_001079"
     CDS             complement(11923..13188)
                     /gene="yihN"
                     /locus_tag="ACHOQQ_RS05395"
                     /old_locus_tag="pgaptmp_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4469358.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVKLKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(13290..14270)
                     /gene="yihM"
                     /locus_tag="ACHOQQ_RS05400"
                     /old_locus_tag="pgaptmp_001080"
     CDS             complement(13290..14270)
                     /gene="yihM"
                     /locus_tag="ACHOQQ_RS05400"
                     /old_locus_tag="pgaptmp_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU4469359.1"
                     /translation="MMTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKKSIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(14278..14988)
                     /gene="yihL"
                     /locus_tag="ACHOQQ_RS05405"
                     /old_locus_tag="pgaptmp_001081"
     CDS             complement(14278..14988)
                     /gene="yihL"
                     /locus_tag="ACHOQQ_RS05405"
                     /old_locus_tag="pgaptmp_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4469360.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(15205..17028)
                     /gene="typA"
                     /locus_tag="ACHOQQ_RS05410"
                     /old_locus_tag="pgaptmp_001082"
     CDS             complement(15205..17028)
                     /gene="typA"
                     /locus_tag="ACHOQQ_RS05410"
                     /old_locus_tag="pgaptmp_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGU4469361.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            17401..18810
                     /gene="glnA"
                     /locus_tag="ACHOQQ_RS05415"
                     /old_locus_tag="pgaptmp_001083"
     CDS             17401..18810
                     /gene="glnA"
                     /locus_tag="ACHOQQ_RS05415"
                     /old_locus_tag="pgaptmp_001083"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGU4469362.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            18984..20033
                     /gene="glnL"
                     /locus_tag="ACHOQQ_RS05420"
                     /old_locus_tag="pgaptmp_001084"
     CDS             18984..20033
                     /gene="glnL"
                     /locus_tag="ACHOQQ_RS05420"
                     /old_locus_tag="pgaptmp_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGU4469363.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            20045..21454
                     /gene="glnG"
                     /locus_tag="ACHOQQ_RS05425"
                     /old_locus_tag="pgaptmp_001085"
     CDS             20045..21454
                     /gene="glnG"
                     /locus_tag="ACHOQQ_RS05425"
                     /old_locus_tag="pgaptmp_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGU4469364.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            21566..21676
                     /gene="yshB"
                     /locus_tag="ACHOQQ_RS05430"
                     /old_locus_tag="pgaptmp_001086"
     CDS             21566..21676
                     /gene="yshB"
                     /locus_tag="ACHOQQ_RS05430"
                     /old_locus_tag="pgaptmp_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGU4469365.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(21866..23239)
                     /gene="hemN"
                     /locus_tag="ACHOQQ_RS05435"
                     /old_locus_tag="pgaptmp_001087"
     CDS             complement(21866..23239)
                     /gene="hemN"
                     /locus_tag="ACHOQQ_RS05435"
                     /old_locus_tag="pgaptmp_001087"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGU4469366.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(23428..23934)
                     /gene="yihI"
                     /locus_tag="ACHOQQ_RS05440"
                     /old_locus_tag="pgaptmp_001088"
     CDS             complement(23428..23934)
                     /gene="yihI"
                     /locus_tag="ACHOQQ_RS05440"
                     /old_locus_tag="pgaptmp_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGU4469367.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(24053..24219)
                     /locus_tag="ACHOQQ_RS05445"
                     /old_locus_tag="pgaptmp_001089"
                     /pseudo
     CDS             complement(24053..24219)
                     /locus_tag="ACHOQQ_RS05445"
                     /old_locus_tag="pgaptmp_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24516..25148
                     /gene="yihA"
                     /locus_tag="ACHOQQ_RS05450"
                     /old_locus_tag="pgaptmp_001090"
     CDS             24516..25148
                     /gene="yihA"
                     /locus_tag="ACHOQQ_RS05450"
                     /old_locus_tag="pgaptmp_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGU4469368.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(25530..28316)
                     /gene="polA"
                     /locus_tag="ACHOQQ_RS05455"
                     /old_locus_tag="pgaptmp_001091"
     CDS             complement(25530..28316)
                     /gene="polA"
                     /locus_tag="ACHOQQ_RS05455"
                     /old_locus_tag="pgaptmp_001091"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGU4469369.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIESGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALDEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAYEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LKYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            28680..29612
                     /gene="yihG"
                     /locus_tag="ACHOQQ_RS05460"
                     /old_locus_tag="pgaptmp_001092"
     CDS             28680..29612
                     /gene="yihG"
                     /locus_tag="ACHOQQ_RS05460"
                     /old_locus_tag="pgaptmp_001092"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU4469370.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRHDK"
     gene            complement(29653..>30903)
                     /locus_tag="ACHOQQ_RS05465"
                     /old_locus_tag="pgaptmp_001093"
     CDS             complement(29653..>30903)
                     /locus_tag="ACHOQQ_RS05465"
                     /old_locus_tag="pgaptmp_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HGU4469371.1"
                     /translation="KGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPITM
                     LMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVASAE
                     YGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLEEK
                     KFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLTKG
                     NQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEMNAALFK
                     EYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVKLP
                     LNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLHYTP
                     GKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         7807 a         7928 c         7414 g         7754 t
ORIGIN      
        1 aaatcgctca aacatgaact aagatgatga attaagcatt atctgcgata attgccatac
       61 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggagc
      121 caacgaagaa taaaaattta tagagttctt attcaatata accgaaatag ccaaggacta
      181 atataaatga cctgaatgag agaggcagca ccgcccctct ccatcagcta ctcttcttcg
      241 ttcccgccct cttcatacgg gccaaatggc ttcgcgggca gaacgatata cgtgccttca
      301 aacactgcac ccggcgtctc gtcgccaaag atttcgacct gcatctgcac ccgtgctttt
      361 cgtccgcgcg ccagacggtc gagatcgccg cttaaggcac cgaggtcggc taccgcatga
      421 ggtttaccgc taatcggctt gctgtagcgg atatgcgcat ccgcaagaat aatcgttccg
      481 ccgaggtggc gttcacgcag catcagccag ataagtcccc aaccggtgag cgtcgccagt
      541 gagaataaac tcccggcaaa cagcgtatgg tgcggattct gattgccggt ttctggcatg
      601 gtagtgataa atttttgccc ggtatattgc tgaatgcgca cgcccatttt ttcactaagc
      661 gggatatgtt cgtaccacgc ctgttgcagc tgcgcgcacc agtcgccgcg atgcagaata
      721 tcatccagag tggcgacggg cttaatcatc aaaaaatggc gaatcggcgt ggtggttggc
      781 gtggtgattt ctccctgatt aacaaacccc agcttggcga aaaactccac cgcgtcttca
      841 cgggcgctac aggtcacgcg cttaacgcct tcctgacgcg ccaccgactc cagggtcatc
      901 gccatcagcg tgcctaaccc tttgtcctgc acgtcgggat gaacggccat aaagcgaatg
      961 gacgcttcat tgtcggcatt aatatacagt cggcctaccg ccaccagatt accctgctcg
     1021 tcgacgacca tctggtgatg cgccatcgca tcccacgcgt cgcgttccga accttttggt
     1081 tgatgcaggg gcttacgcaa catttcccag cgaaactgat agtaacgctc taattcttct
     1141 tctgtttgtg gaacccgaag gtgatacata gcgatactct ctcttgtttc ccgtgacaac
     1201 cctggaagct ggctcatacc tgcaaccaga atgtcacggg gccatcattg accagcgata
     1261 cctgcatatc cgcagcgaag cgtcctgttt gcgtgttcat ctcttgctga cggcagcgtt
     1321 cgacgaaata gtcatataac gcctctgcgc gatccggtga tgcacctttg gagaaacttg
     1381 gacgcatccc ccgttcggta tctgcggcga gggtaaactg ggaaaccacc agcacactgc
     1441 cgcccgcctg ttgcacgttg aggttcatct tgccttcggc atcgctaaag atgcgatagc
     1501 cgagcacacg ctcgcacaga cggtttgctt tctgttcgtc gtcatccttt tcgacaccca
     1561 ataacaccaa aagtcccgcg ccaatttcgc ccgtcacttc tccctccacg gtgacgctgg
     1621 cacgggttac gcgttgaatt aatgcaatca tggttcgtcg tcttcttctt gttcagctgc
     1681 ttgttttagt ttgcggtatt ccccgagagt gacagtaatt tccgcgccaa gcaagacgat
     1741 acaccatgtc cagtagaccc aaacaaagag aatggggatc accgccagca caccgtaaat
     1801 gagctgatat gacgggaaca tggtgatata aagcgcgaaa cccttctttc ctgcttcgaa
     1861 caggagcgcg gcgacaaacg cgccgacaat cgcgtcgcgg ttaggtacgc ggatggtagg
     1921 aacaatgctg tacagcaacc agaaggagat ccacgacaac agcagcggaa aaatacgcag
     1981 cacattgtcg atgacagtat tgagatcgct cgcccagcgc agggagagca aataggaact
     2041 gatcgccaga ctggcccctg ccagcagcgg ccccagcgtt aaaatcatcc agtacacggc
     2101 gaacgagtaa atttttggtc gagctcgttt actgcgccag atggtattca acgcgctatc
     2161 gatggagtac atcaacaata acgcggtgac gatcagcccg cacgccccaa cggcggtcat
     2221 cttgttggaa ttggcaacaa attgttcgat ataccgctga ataacatcgc cagtagcagg
     2281 cagaaagttg gcaaaaataa agtgacgtaa ctgaatgctg acgtcggaaa acatgggaaa
     2341 agcggcgaaa agcgcaaaaa caacggcaac cagcggcact aatgagagca acgacacata
     2401 ggcaaggtta cctgccaggg ttgtcatatt gtcctcatca atgcgttgcc agagtagttt
     2461 tagccaggcc catagtggac gggtacggtg cctggctttg tcctgaatgg tttttagcat
     2521 aacaccttcg cgaaatagtc cgggatggtg gttttatctt tcaccagaat actggtaatg
     2581 cctagctgat tagctccttc tatattatcg gcgttatcgt cgaaaaaggc cgtatcgctg
     2641 ggtgaaaaac cttccgcctg caaaacatgc tggtaaattc gagcttcagg tttgcgcatc
     2701 cccagatctt gcgacagata gatatggtca gcagcatcac gaatttccgg gtattcttcc
     2761 ggccagaagg tggtatgcag gcggttggta ttggaaagca ccaccacgcg atgcccctgc
     2821 tcacgcagtt tatgcatgat ggcgatcact tccgggcgca gcgcaacaaa caccgcctgc
     2881 cagccgtgag agaactgctc gtagcttagc ggtagagcca tctcatgaca cagcgcctct
     2941 gcgaacgctt cgtcgctaat ttccccacgc tcatgctgat gaaacgcctc ccccatatga
     3001 aaactcttct taagcgtagc cagcggaata cgcgttaaat cgctccaggc tcccagcaca
     3061 cggttaaagt cgatatcgac aatcacatta cctaaatcaa agatatagag catttttgcc
     3121 tcctttcgcg ccacaagaaa ttaactgtag cgggaaagga tgggtttggc tatgtggcaa
     3181 taagggtaat cttgtgatgc tgattgcgaa aaagcagagt aatttcactg gctttttcgc
     3241 aatagatgaa tggttaagat tcaggtgtaa attgccaacg agtttcgata accagcggcc
     3301 tatctggtgt caccaattcc ggcgttagat ttattgcgtt aggtgcctga gtcagcgggt
     3361 taatacaggt ggcatcgggc tgtttatcaa ataccaccag actgtttgca gaagagatca
     3421 tggtcatcgc gagtttacca ggccacagta acttaacctt caccccgtca taaaaaccaa
     3481 aacagtcatc ccagggaccg acttgaggag agatgcgatt tccagttggc agttcatcac
     3541 tacccgcttc ttcttgccag tccgcatcaa aaagcacctg caacgattct gtattttgcg
     3601 gagtcagctt tttcgcaaac cacggatgcc agcctgcgga ggccgggaat gtatcggcat
     3661 atgaatggac ttccagttgc aaaaccagcg catcgttctc cagcaaaaaa gtctgaatca
     3721 cttccccttg ccaaggccac ggtgaagcca ggggcatacg taaagtcagg gaatctgctg
     3781 ttttgtcgat gatttcccac gttgagtaac aagccatacc atgcaacgca tgtggtggtt
     3841 tattggcggg taaagaataa cattgcccac cagcattcag cgtcgcattc cccagtcgcc
     3901 ctgcccacgg caccattggg aaacagccat actgaaaagc tctacgctgc ggctgccatt
     3961 ggcgtaatac ttcagaacca aatgccttta acgaggttat cctggcacca tcatgcggat
     4021 aaatctgcag gtgaatattg cctttttcca gcgtcactaa ctctcctaca ggcccaggcc
     4081 atggcgcgtc aaacgcctgt tgtagcgcca ggtaatgctc gttttttcgt tcagttttca
     4141 tgagattgtt ccctgatgta gcggtaagaa gcggtttctt gttcatcggt cggctcaatc
     4201 agccagacgc aaacaaagga aagaactgag acagcaaata aaaacagagc gacataccac
     4261 ggttttcctc cccctagcgc cagcaaactt gaggcgataa gggggcttag tccgccaaga
     4321 atcgcggaaa attgataagc aaaagaaagg ccggtatatc gtactttggt accgaacatg
     4381 cgggtgaaaa gtgttggctg cacggcaaac atcatcgtcg gacctaagtt gtagccgagg
     4441 atcatactgc accagatgat gagcgtactt ttgctatcca gtaataagaa gaaaggaaac
     4501 gcgaacagga cacaaaatcc tgctcccgac aaatacagac ttttttgacc tatacgatct
     4561 gataaccatc caaccaatgg acaactgaaa ataccgatcg ccgaggcaat caacataccc
     4621 gtcaggggaa tatctcgcga taatgctaat tggctggaaa tatagacaat accaaaggcg
     4681 ttaataatat tagaggagac actttccgcc agcctcgcac ccagtgccaa taaaatattt
     4741 cgtggatgtt ttttgaataa ttcaatcaac ggaggaacgg acttttcttt attattcgtc
     4801 gttttttgct tttgaaaatc cagcgtttct tcggtatgca ggcggataaa cagcccaacg
     4861 atcaacatca ccgcagaaag ccaaaacgga atgcgccatc cccaggagag aaactgctcc
     4921 gaagtaagaa aatggttaca gagagcaaaa ataccggttg ccagcataat acccacagac
     4981 gcagccgttt gcgggagtga acctaagaat ccgcgtcggg attcgggcgc tgattctatg
     5041 gtcatcaatg ccgctccacc gtactcgcca ccaagaccaa agccctgaat caaacgcagc
     5101 accattaata caagcggtgc ccaaatgcca atttcctgat acgtcggaat aaaaccaatc
     5161 aaaaatgtgg agcaaccgac aatagccagc gtccagataa gtgttatttt tcgcccgatt
     5221 ttatcgccaa aatgaccaaa gacgatgccc cctaaaggac gagtcagaaa accgacacca
     5281 aaggtcgcaa aggaagctat aagacctata aaaggatcgc tgtctggata aaacagcggt
     5341 gcaaatacca gtcctgctgc cgtgccaaaa ataaagaagt cataccattc cattaacgcg
     5401 cctgcgaaac tgcccataac gacacgtctc atggctttac cggaaagccc ttcacgcggt
     5461 gcgggtaact tttcagacat aattttcctc tcctggtagg cgagaaatct gggatacgaa
     5521 ccactaaacg tgggcagttt gaattaatgc gcatcggctt aagccgatgc gcattaaatt
     5581 atttcttcaa ttcagccaga acctctgcgt cctcagcatc aacatttgca cccagtggtt
     5641 cccaatgaat caatacgcag gtcaggaacc ccaggaatgg cacgattgcc aaccagtaaa
     5701 acgcttcagt tttcaacgca ccccacagca tcggccagaa aatgagtcct gcaattgccc
     5761 ctgtgcgcat aatagcgcgg gcaaatccga ccccagtagg gcgaatagcg ggaggataag
     5821 agagtgtggc aatggtcatg cccagcccac caggacccgc tgaatggaag aaaataattg
     5881 atcccaataa ccctaaagac agccagggat tagtcgttgc cagtgcgcct aacgacagca
     5941 atgctaaggt cacaacagca aatccataca tcgactgtcg gcgagtaccc agtcgttgga
     6001 gaataaacga gccgatcatc ccgcctgtca ccccacaaag ggtattcacg acggcagaac
     6061 cggtcaacgc gccagtaagc ccgccactta ttattcccgc caatgtaaga ggaagataaa
     6121 cacctacggc gttatattgc cacgcctgca tggttgccac gacacagcca agaatggtgc
     6181 gtttgcggta acgatcatta aataagatgc catatccacc actgtattta ttctctgctt
     6241 tttccgagaa tttcgcttct gttgtactcg atgccgcaac gcgagcctga acaccataac
     6301 gttttcccag aatgtcgcac gcttcctggt aacgcccgac gcgtgcggcc cacattgcgg
     6361 attcaccaat aaagaagtaa cgcaaaatca tgaagatgac tgcaaaaata gctccgatga
     6421 atattccgta acgccacaat tgttcttcag cgatacccga cagaagcagc ggcaaaagta
     6481 ccaaataaac aacggttgtc gaaacatacc aggccatttg ccagaggttg accgacgttc
     6541 cggttttctt cgacgttgaa ccacgaagct ctgccacggc attggttgcc aaagggaagt
     6601 cgatgcctaa accaaagccc atgatgacgc ggcacaccag cacccaccag atactgggag
     6661 caatagctac cgctgcagca ccgatggtgc acagccccat gccaataata aatgcccgct
     6721 tttgcccaaa gcggtttatt actcggttcg ctaataaacc gccaattaac gcggcgatta
     6781 atactgatgc attgacggtt gcagcaagac ccggcgaaat gccgaattga gcggcaatgt
     6841 atttattacc aaaccccacc atcgccgcct ggtaggcatc aatcaatatg ccgcccaaag
     6901 caataaaaat aacgccgata ctggtgttga ttgcagagtt cttattaaca atatcaatta
     6961 cgtcctgtgg ccgactaatt gaataagttg caggactggt gatttgactc atcgcagtgt
     7021 cctttttaat tgttgtaggg tggcgctgac gtgacgcagc gccagacaga gatttagaaa
     7081 atcattccgg cgcaaattgc agcatgacct ttatggagtc attttgctgc gcgcgttcga
     7141 atgccgccgc tgcatcctca atggcaaata aatcggtaac cagttcactg gtatcaaacc
     7201 gcccatccgc cagccattcg ataacagcat cgaaatcttc agggatgtac atgccagagt
     7261 tgagcaagtc acgctcgaaa cgttgcatca tcggtaatgg tatttcacgc ggcccacttg
     7321 gtacgcccat gcacacgacg gtgcctccgg catatacacg ggtacatgca tcgttaagcg
     7381 tggcctgcgc tgcaacgaca tcaatcgccc ccgtaaatcg cacatctgcg gccagctcac
     7441 ctggtgccca aacttctgct gcgcccagct ttaacgccag cgcgcgtttg gcagcgtcag
     7501 gttcaatcac ggtaatctta cctgccccca taatgcgcag tgcctgcacg atggataagc
     7561 ctatcgttcc ggcaccaatc accagtacgc tttccagaga agctttcggc attcggttaa
     7621 cgcagtgacg ggcacaagcc gccggttcgg caaaggccaa cacttttagc ggtaaagagg
     7681 ctggtgcgac atggcaatta cgcgcaggaa caatgtgttg tgagcgagca aagcccggtg
     7741 cgcgaaaacc agcaacctgc ggtggttcac aaagattaaa acgtcctgct ttgcaggctc
     7801 ggcattccat gcaagccatg atgggatcga ccacaacatg atcgcccggc tgtacatttt
     7861 taacgtcgct gccaacttcc gtaacgcgcg ctgcaatttc atgaccgggg acgacaggtg
     7921 gtttggcaaa cggatgaccg cctttcagaa catgcagatc cgaaccgcat atcccataga
     7981 acacaggcgc aatccgcact tcgtgcggtt taagttcctg gtgtggtact tcaacatatt
     8041 gagtggtgac tttcccaata tctgaaaata aaacctgggt aattttatcc attgttattc
     8101 tccagaatcg atatccatag gggattagcg ttgcgcgata ccttcatcga caatttctgc
     8161 caaccgttgt aaacgaggaa cagagacatc gcgcagcata atttgtggat cttgtgcacc
     8221 aacaaccgac gcccaaaccg ctcgtccagc caggaagcct gacgcaccac ctttcatggc
     8281 aatacttact gcacggccaa aaatatcggc atcaacgcca gaagaaagaa ttacccacgg
     8341 catcttcatc tgatcgttaa gttgctgaca ggcggcaagt aaggtttttt catcgccttt
     8401 gccccctaat ggcatctcgg ctttgtaaag atcagcctct gttccaccaa gctctgcggc
     8461 ggcggcaaca atggcgcttt cacgatcaaa gtcccaaccg cgacgaggtg gacgcaccac
     8521 aggttcaata atgctaacca gcccggcact acggcagcga cgaacaaatt tatcaaccat
     8581 tgccagacgt tcctctgcgg gttcatcttc acgccagagt acaagtaatt tcattgcctt
     8641 agcccccatt tcacgggctt tatgcgggtc gacggacata tctatttcaa cgctatcaac
     8701 cgggatgcca ttacctggaa taaaaagatc ggcagcgaca atcaggccgc aagaattagc
     8761 aacagcgccc tgctcgacta tttgctcaag acaaaattgt ttatccgcca gaacggcaga
     8821 agcataagga gaaaggattc gggtcgcggc aactttaaaa tcggtgagta cactgtcggc
     8881 gatgggtttt ggctgccctg ctgccgcaaa catcaagcgc atggcctcac gctgatcgac
     8941 agctaacatg gcaaatccac cacctgggcg ggagatatct ttcagcgtaa atggggtgaa
     9001 gtgggatgac atagaaactc ctgttaatag ataaaattaa agaccagcct gctggcgaac
     9061 ctgatttagc agggcactcc agtcctgccg ccctttgccg tcttgacttg ctgcagcgta
     9121 ataactttct gctgcaatcc ccatattgag cggtgccccc gtctcccgag cgacgtcaac
     9181 tgcaatacgc agatctttca acgccagatc caccatgaag gcgggtgaaa gatcgccttt
     9241 cagcactttg ccaggccagg tggtcgtaaa atgtcctttc cctgctgccg taccactcat
     9301 caccttaatt gcgacatcaa gatttaatcc cagtgactca cataacacag cggcctcgga
     9361 ggacagcgca ttaagcgcaa tgctcatata gttgttaatt agcttcacgc ggatccccat
     9421 tccagggccg ccagcctcga ccagctcatt acccatgcac atgagaatcg ggcgtgcacg
     9481 tgcaatctgc tcatcagtgc cacccgctaa aatcagcaat gtgccatcaa tcgcattcac
     9541 ggacgttctg cccacggggg cgtccatcat actgatgcct ttttcttgta actcccgtat
     9601 taatgcatcg gtctgtaagg gatgaatagt cgacatatca atcaataacg cgtcagatga
     9661 aacagcctca cagacgcctt tttcccccaa cagaacctgt cgaacaatgt caccattggg
     9721 aagcatagtg atgataaatt cagcgcctgc tgctgcttcc gctggggttt gtgccgcagt
     9781 ggccccctgc gtaactagcg catcgacggc cagtgcattg acatcaaata cctgcaatga
     9841 atgcccttta tgcaatagat tgttagccat tgggctaccc atctgcccca gcccgagaaa
     9901 tgcaatggtt gccatgaata ctctcctgtc tgacattgac aaaataatga caattgaaaa
     9961 ttcgttttcg tctgtttttg agcatatttg taatttatgt tcaaaaacac gacatacatc
    10021 actaatttga tcatttgtgt catttaaatt gaccaaagac gacaaaatta aacaggacaa
    10081 gacaaatatg accagaattg catgcgtggg tatcacagtt caggacagga tttattcttt
    10141 gcccactctg ccggagggcg gcgggaaata tcaggcaaat cattatcttg agattggtgg
    10201 cggcccggca gcaacagccg ctgttgcaat cgcaaggctt ggcgtggaag ttgatttcat
    10261 cggtcgcgtc ggcgatgaca gttgtggaaa taccttactt gcagaacttg agggttgggg
    10321 cgtcaataca gcattttgcc gccgatatcc taacgcccgt tcgtcgcaat cagcgatcct
    10381 cgttgatcag catggcgaac ggattatcgt taattatcca agtcctgatc tcggtactga
    10441 tgccgaatgg ttagaagcga tcgatttctc tcgttatgat ctgattctgg ccgatgttcg
    10501 ctggcatagc ggtactgaaa aagcattctc gctagcgcgc cttgcgggtg tgacaacgct
    10561 tttagacgcc gatatgacac cgcaggatat ttctccactt gtcgcgcttg ccgatcacgc
    10621 cgtgttctca acgccagggc tgaaacgcat gactggcctg cagtcacctg aagaaggttt
    10681 gttccaggca acgacacaaa cagcaggtaa agtgtatgtt acgctgggtt ctgaaggatc
    10741 gttgtggatt gaagatggtc atttgtgtca acaggaggca ttttctgtca atgtggttga
    10801 taccactgga gcaggcgatg tttttcacgg cgcactcgcg gtagcgttgg cagaaaaaat
    10861 gccaacaaaa gaggctattc gttttgccag tgcggttgcc gcgatgaaat gtacccagcc
    10921 tggcggtcgg gccggcattc ctaatcgtga gcaaaccgaa tcatttttgt cactttatgc
    10981 gtaaaatggt aaacatctac cgaggagggg caagggagta ttcatgagta tcatcgaagt
    11041 gacaggtaat ccacgccatg accaactcgt tcatctaatc gcagaacgcg gttatatgaa
    11101 tattgaagaa ctggcacaac tgctggatgt ttcaacgcaa accgtgcgcc gggatatccg
    11161 aaaactcagt gagcagggct tgatcacccg tcatcatgga ggggccgggc gcgtctccag
    11221 cgtcatgaat accgcttttg agcaacggga actttcgctc accgccgaga aacgggcgat
    11281 cgctgaggca gtcgccgatt accttcccga acgctgtacc gtctttatca ccatcggtac
    11341 aaccgtagaa gccgttgcca gggcattact caaccggcgt gatttacgca ttatcactaa
    11401 cagcctacgt gtggcacaga ttctttataa gaatcaggat attgaagtga tggtgccggg
    11461 aggcacttta cgcgctcata acggcgggat tatcggccca ggagccgtgg attttattga
    11521 aggcttccgc gcagattatt taatcaccag tataggtgcc attgaacatg acggcaccct
    11581 actggaattt gatgtaaatg aagcgttagt cgcgagaacg atgattaaac atgcgcggaa
    11641 tacattgtta gtggccgatc atacgaagtt tgccgcgtct gctgccgttt caattggcaa
    11701 tgcacggaat gtcagggctt tttttactga tgccccgcct cccaattctt tctgccagtt
    11761 gttaagtgaa gagaatgttg aactggtggt tgccgagcag gaagtttcct gaaattgaaa
    11821 cttcgaaaaa tccgctgggg attcagcgga ttttattttg ccggatgcgg cctacggttg
    11881 atacggtttt cgttggccgg attaagcaca tccggccatt aattaagctt cagccgccag
    11941 catctctttc ttacgttggc taatacgctt aaccagcatt acggaaacca ccgcaccaca
    12001 gaatgcgaag caggccataa tgccaaacac gattttgtag ccaataatcc ccggatttaa
    12061 atccagaatg tagccataca gactgaagca gaacatcgcc ggggcgtaac caataaagct
    12121 acccaacgcc atcgccgcgc ctgttttatt ttcagcaatt tttgcttcgc cgataggtgc
    12181 aaaaaatacg gctcgctgtg taaagactat cgcgccaaag cccagcgtac atgccatccc
    12241 taaatagacc ggcatacttt cgtgaggtag cataatcaac agtacgagcg cagcggtact
    12301 gatgataaag gtgtagcata gatatttact cggcgatttc aggatcttat ctgaaatcat
    12361 gccaccaatc ggtccaccaa tcattttcag acagtattgg ttaatgatgc cgtaagcccc
    12421 caccagcgca acgggcaata gatagatgtt tttcaggaat ggaatgaaga atgtcaggcc
    12481 acagtaaacc gcgtaaacga agaagacgtt gaaagcgata agccagatag ttttgtcttt
    12541 cagcaccgag gtcatactgg tgttttgcga tgccccgtct tcttttttca acttaacgga
    12601 cggtgcctct tctttgtcat tcaggacaaa gaaaataata atacctacgg caatcacaat
    12661 aagggaatag aaccagatgc ctgctttaaa acccaataag ccactgccaa accaggtaaa
    12721 tactgccaac gcagaaaatg ccaccacggt atcgacaatg ccacgccctg tttcgaagaa
    12781 gccaaacaac cgaccttgtt gttcgctatt gcccaatcga cttaccgact tgagcaagac
    12841 cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc cagacaaaga ggatgcccca
    12901 ataccccggc attgtcgtca aataaacacc cagtaatcct gtcgcaatga gtgagaatga
    12961 catggtgtat ctgcgcggta gtttatcagc aaaataaata gacagaaaaa agcccactgt
    13021 ggtaacaaat gagtttaccg acatagcatt accaatttga ccattggtca aatggaaata
    13081 ttcctgcatc gggatataaa acgcatcttt cagcgacggt aatttataaa ttgtaccgcc
    13141 acacagtgtt aatagactaa ataacgccca tttctttttc gtgagcataa catcccctaa
    13201 gcgattttta aaatcactgt tttaaaggca ataagatacc gaccattcat atggaatggt
    13261 tttattaact gcgtatgagt taaaacttat tagttgttca gaagatggtt tgcctcttgt
    13321 ttgatttgtt gtagtacgtt acgcacatga tttatctgat taattgcatc ttctttttct
    13381 ttacgtaacc gcgcatccag taaatgattt tctggtaggg gtgtttcact catctggcga
    13441 taagggatcc acggattatc atcttcgtct tcaaagcgga acgccggcgc atagtaatca
    13501 acctcttctt ccaggccata tgccgtcacc tgcggctcat catcacccag gcagataagg
    13561 tgcgttaata acgctttgaa cggcatatca ccctgacctg aaatacaggc tttatgaccc
    13621 aggccaccct gttctttaac gatcaaggca tctttaatat ggacctgggt aatatgcggt
    13681 gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat tgatcatgtt cgcaaaatca
    13741 aacagtaagg aaagagactc catctcactt tctttgacca gcgacaccaa ctcatgactt
    13801 tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc tgtcctgata cgtttctcgt
    13861 acataggcaa tgtcgttagc gataatcgat aatacgtcgc gcaaattacc ttcataacgc
    13921 ggataaaaac gtacggacga tgccccagtt ttcaacgcga tggcgacggc ttcgtcaata
    13981 gattttttat ctgaggcgct ggtttcaata tgaatatcaa ggttcagacg gcgggcttta
    14041 tccccaaagg cagagagttc tttatcgtcc atattaccaa gagaaaaacg ctcgccatcc
    14101 agaacatgga ttttgacgcc acgtagatta ttttcacttg cgatatcaag taaatcagca
    14161 ggcaacactc tttccagccg catatttaaa tgaaaggcat aagcatgtaa ataaagagga
    14221 agagtgtcga cacgttgtag aatctttctt gcgttattta ttgtcatcat aaaacaccta
    14281 cagattaatg atgaggtcta cggcctcaaa tcgccaatat tcaatatcta tctggacaat
    14341 attgccttgc tggtcggcgc gatgcttttc aattaagatc gctggcatac ccgcagaacc
    14401 gccaagatac ttgctgatat cgcccggcat tctgacgggt ttgaaaatca atttttttac
    14461 taccgtctct ttttggtagc acttttccca gactgcagaa aatgagtggt tttcaagttg
    14521 ttcaataaaa cccggagcaa cttcaggatt aatatatgtt tcgtgataga aaactttatg
    14581 tccttccagc gccccccagc cggtgatgcg atagagttca gttgatggtg tcactgcaat
    14641 cagtggcgcg agcgtctcgg gaatatcgct ggtacggctt ttctcggtaa acccccagga
    14701 aggttcccgc ccttgctcaa ttgcggcccg ctgaaagctc gccgacagct ccggactgta
    14761 attaaatcgc ggctgggtca cgaaccaccc cttacgatcc ttacggaaga ttttggattc
    14821 tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt ttaatgccca gcagttctcc
    14881 cagctctctt tctgaaggga gttttccacc cggcgtggtg atgccatctt tcaaccaccg
    14941 atccagtttc tcttttgcat tttctacggt ggattgattc tccgccattg tttaatcctt
    15001 ttggtttaaa ccagatgaag catcttacgc ttacgccaaa aaagaaagca attaacggca
    15061 tatgactttg cagataattg tgacaaagct ctctattgac gtaaccacag acttataagg
    15121 gagatactcg cattggtgtg cgctcacgac aggcaaaaaa aaacctgcca tcgctggcag
    15181 gttttttata actaaaaaac gaaattaatc gtctttcggt gcgcggttgg cgcgacgacg
    15241 atcgttttcc gtcaggtgac gtttacgaat acggatagag gtcggagtca cttctaccag
    15301 ttcgtcatca tcgatgaact ccagagcttg ttccagagtc atgcggatag gcggaaccag
    15361 aacaacggct tcgtcagtac cggaagcacg catgttggtc agtttcttac cggtcaggca
    15421 gtttacagtc aggtcgttag agcggctatg aataccgata atctgacctt cgtaaacttc
    15481 tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga ccgaacagcg cgaacgcgac
    15541 cgctttaccc tgaccgttag agatcagtac accgttctga cgctgaccca cttcgcccgg
    15601 acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga cccgtaccgg aagtcatggt
    15661 catgaactca gaacggaagc caatcagacc acggcttgga atcacgtagt cgagacgtac
    15721 gcggccttta ccgtctggat tcatgttttt caggtcgcct ttacgttcgc ccagcgcctg
    15781 cattacagaa ccctgatgct gttcttcaac gtccagagtc acgttttcat acggctcttg
    15841 tttacgaccg tcgatttcac ggaagataac tttcggacgg gataccgcca gttcgaaacc
    15901 ttcacgacgc atgttttcga tcagaacaga caggtgtagt tcgccacgac cagaaacgcg
    15961 gaacgcatca gcgtcttcgg tttcttctac gcgcagcgca acgttgtgta ccagttcttt
    16021 gttcagacga tccaggatct gacgagacgt tacgaactta ccttctttac cgcagaacgg
    16081 cgaggtgtta acgcagaaga acatagaaac ggtcggctca tcaacggaga gtgccggcag
    16141 cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg ttcagttcgc ccaggcccgt
    16201 gatcgccacg atatcgccag cttccgccag atcggtttcg atacgttcca gaccgaggtg
    16261 gcccagcact ttaccgactt tcgcgttgcg ggttttgcct tcgctatcga tgatagtgac
    16321 ctgctggttc ggcttcactt taccgcgctt gatgcggcca atgccgataa cgccaacata
    16381 gctgttgtaa tcgagctgag aaatctgcat ctggaacgga ccgtcaaggt caacgtccgg
    16441 cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc atgtcttccg ccatatcttc
    16501 gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa acgatcggga agtccagctg
    16561 ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc tgatccacaa cccaatcagg
    16621 acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc ggcttcaggc cgtaagcaaa
    16681 cgcttttttg gttacgaagc gcgtttgcgg catcgggccg tcaaatgcgt caaccaccag
    16741 cagcactgag tctaccatgg acattacacg ttcaacttca ccaccgaagt cggcgtgccc
    16801 cggggtatca acgatgttga tacggtaatc attccatttg atagcggtgt ttttcgcgag
    16861 gatggtaatc ccacgctctt tctccaaatc gttggagtcc atcacgcgct cttgggtttc
    16921 ggcacgagag tcgaacgtac cggattgttg gagcagcttg tctaccaggg tggttttacc
    16981 atggtctacg tgcgcgatga tggcgatatt acgcaatttt tcgatcacaa ctttgcctca
    17041 ggcattagaa atagcgcgtt attgtacacg gattaatcgc actacaaaac aggatcacaa
    17101 acatcctccg caaacaagta ttgcagagtc cctttgtgat cgctttcacg gagcataaaa
    17161 agggttatcc aaaggtcatt gcaccaatat ggtgcttaat gtttccattg aagcactata
    17221 ttggtgcaac attcacatca tggtgcagcc cttttgcacg atgttaagca tgataacgcc
    17281 ttttaggggc aatttaaaag ttggcacagt tttcgcttta tcttttttac ggcgacacgg
    17341 ccaaaataat tgcagatttc gttaccacga cgacaatgac caatccagga gagttaaagt
    17401 atgtccgctg aacacgtact gacgatgctg aacgagcacg aagtgaagtt tgttgatttg
    17461 cgcttcaccg ataccaaagg taaagaacag cacgtcacta tccctgctca tcaggtgaat
    17521 gctgaattct tcgaagaagg caaaatgttt gacggctcct cgattggcgg ctggaaaggc
    17581 attaacgaat ccgacatggt gctgatgcca gacgcatcca ccgcagtgat tgacccgttc
    17641 ttcgccgatt ctaccctgat tatccgttgc gacatccttg aacctggcac cctgcaaggc
    17701 tatgaccgtg acccgcgctc catcgcgaag cgcgctgaag attacctgcg ttccactggc
    17761 attgccgaca ccgtactgtt cggaccagaa ccagaatttt tcctgttcga tgacatccgt
    17821 ttcggatcat ctatctccgg ttcccacgtt gctatcgacg atatcgaagg cgcatggaac
    17881 tcctccaccc aatatgaagg tggtaacaaa ggtcaccgtc cagcagtgaa aggcggttac
    17941 ttcccggttc cgccggtaga ctcggctcag gatattcgtt ctgaaatgtg tctggtgatg
    18001 gaacagatgg gtctggtggt tgaagcccat caccacgaag tagcgactgc tggtcagaac
    18061 gaagtggcta cccgcttcaa taccatgacc aaaaaagctg acgaaattca gatctacaaa
    18121 tatgttgtgc acaacgtagc gcaccgcttc ggtaaaaccg cgacctttat gccaaaaccg
    18181 atgttcggtg ataacggctc cggtatgcac tgccacatgt cgctgtctaa aaacggcgtt
    18241 aacctgttcg cgggcgacaa atacgcaggt ctgtctgagc aggcgctgta ctacattggc
    18301 ggcgtaatca aacacgctaa agcgattaat gccctggcaa acccgaccac caactcttat
    18361 aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg cttactctgc acgcaaccgt
    18421 tctgcgtcta tccgtattcc ggtggtttct tctccgaaag cacgtcgtat cgaagtacgt
    18481 ttcccggatc cggcggctaa cccgtacctg tgctttgctg ccctgctgat ggccggtctt
    18541 gatggtatca agaacaagat ccatccgggc gaagctatgg acaaaaacct gtatgacctg
    18601 ccgccagaag aagcgaaaga gatcccacag gttgcaggct ctctggaaga agcactgaac
    18661 gaactggatc tggaccgcga gttcctgaaa gccggtggcg tgttcacgga tgaagcgatc
    18721 gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg tgcgtatgac tccgcacccg
    18781 gtagagtttg agctgtacta cagcgtctaa tagttgaagt tgtactaccc ggcgcaacaa
    18841 cgccgggatt tagttgccgt ggaaactttc agcccatctc tgcatgggct tttttctccg
    18901 tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac taaaatggtg
    18961 caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct gggcagatcc
    19021 tcaactcgct gattaacagt attttgttaa tcgatgacaa cctggcgatc cattacgcca
    19081 accccgctgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt acgccgttac
    19141 cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagc ctggaggcgg
    19201 ggcaaggttt taccgataac gaagtgacgc tggtgatcga cgggcgctcg catatccttt
    19261 ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggcg ccgatggata
    19321 accagcgccg cttaagtcag gaacagctac agcacgccca gcaggttgct gcccgtgatt
    19381 tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc
    19441 agcttctcag caaagcatta cctgacccgt cactgctgga atataccaaa gtgattatcg
    19501 aacaggcgga ccggctgcga aatctggtcg accgtctgtt ggggccgcaa ctgcccggta
    19561 cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt ggtgacgctg gtgtcgatgg
    19621 aactgccgga caacgtgcgg ttgattcgtg attacgaccc cagcctgcca gaactggcgc
    19681 acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc
    19741 tggggccgga gggtggagaa atcattctgc gtacccgcac cgcgtttcaa ctgaccttac
    19801 acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg ccaggcattc
    19861 cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg
    19921 ggcttggctt atccatcgct cgtaatttga ttgatcagca ttcaggcaaa attgaattta
    19981 ccagttggcc agggcatacc gagttctcgg tttacctgcc tatcaggaaa taaaggtgac
    20041 gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc gttgggtgct
    20101 tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca tttgagaacg gcgcggaggt
    20161 actggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc gtatgccggg
    20221 aatggacggg ctggcactgc tcaagcagat taaacagcgc catccgatgc ttccggtcat
    20281 cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac aaggggcgtt
    20341 tgattatctg cccaaaccgt ttgatatcga cgaagccgtg gcgctggttg agcgcgccat
    20401 cagtcattac caggaacagc agcagccgcg taatgttcag cttaacggcc caacgaccga
    20461 tatcatcggc gaagcgccag ccatgcagga cgtgttccgt attatcggtc ggctttcgcg
    20521 ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac tggtcgctca
    20581 tgccctgcat cgccacagtc cgcgagccaa agcgccgttt atcgcgctga atatggcagc
    20641 tatcccaaaa gatttgatcg aatcagaact gtttggtcac gagaaaggcg cgtttactgg
    20701 cgcgaatacc attcgtcagg ggcgttttga acaggctgat ggcggtacat tattcctcga
    20761 tgaaattggc gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg
    20821 tcagttttac cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc
    20881 cactcaccag aatctcgaac agcgggtgca ggaaggtaag ttccgtgagg atctgttcca
    20941 ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgt gagcgtcggg aagatattcc
    21001 ccgcctggcg cgccattttt tacaggttgc cgcgcgagaa ctgggcgtag aagcgaagtt
    21061 gctgcatccg gaaaccgaag ccgcgttgac gcgtctggcg tggccaggca acgtgcgcca
    21121 gctggaaaac acctgccgct ggctaacggt aatggctgcc ggacaggaag tgttgattca
    21181 ggatttgcct ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc
    21241 ggacagttgg gcgacgcttc ttgcgcagtg ggcagacaga gcgctacgtt ccggtcatca
    21301 aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga ccgcgttgcg
    21361 acatacgcag gggcataaac aggaagcggc gcggctactc ggctggggcc gcaacactct
    21421 gacgcgtaag ttaaaagagc tggggatgga gtgattcacg gcttgtgtgt aaagattgat
    21481 tattgagcgc aaattgctgg tattttgcgc tttactgttc cgataagttc agtatgatct
    21541 tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg tatcgagtgg
    21601 cgcagttgac agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg ccgcgatgat
    21661 tgggctgttt agttgagttt cagattgctg atgaagtgct ctttgttcat gccggatgcg
    21721 gcgtgaacgc cttatccggt ctacaaaagc aatattttca acaggttttg taggcctgat
    21781 aagcgtagcg catcaggcaa tcttgttctc agcacattga tggcgctttc gttttctact
    21841 ttgtaaacga agcgccattc actatttaaa tcacccgaga gaactgctgc atccgcgctt
    21901 tctggcgcag ataggtatca aagcacatgc aaatgttgcg gatcagcaag cgacctttcg
    21961 ccgtcacctg tattcccttc tcatccacat ccaccagccc atcttttgct aacggggcga
    22021 gcagcttgag atcttccgca aagtaatcag cgaagtgcaa atcccactgt ttctcaatag
    22081 gggcgtaatc cagacggaag ttgcagatga gcgacttaat cacatcgcgg cgaatacagt
    22141 catcacgcgt tagcgcaata ccacgccaca gcgcattgcc ttgttcatcc acttgctgat
    22201 agtactgttt caactctttc tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg
    22261 aaacgcccat ccccagcaga tcggtatcgc cctgagtggt gtagccctgg aaattacgat
    22321 gcagcacgcc ttcacgctgg gccaccgcca gctcgtcatc cggacgggca aagtgatcca
    22381 taccgataaa ctgatagccc gattgcgtca ggaaggcgat ggtttcctgc aggatatcga
    22441 gtttttgctg cggactcggc aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg
    22501 tcggcagatg cgcatagtta aagacgctca gacggtcagg gtttagctcc gccacgcgtt
    22561 tcagggtaaa ggcgaaactt tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt
    22621 tggtggaggt aaagccaatc tcacgcgcat ggttaagcag cgcaaagata aactcttcat
    22681 cctgctcgcg gttaaccaga cgttgcactt ctttgttgaa gtcctgcacg cccatactca
    22741 ggcgattaaa gccctcggcg cgtaaatgat cgagtacatc cagttcgatt tcccgcggat
    22801 cgacttcgat cgaaatctcc gcatcggcat tgaactggaa gttttcgcgc agcagcttca
    22861 tcaggcggct gatttgcgct ttattaagat acgtcggcgt tccgccgccc cagtgcagct
    22921 ggctgacgtg acgcccggcg aacagcggtg cacgatggac gatttcttgc tccagcgcgt
    22981 ccagatactg atcggccttg tgctgctggc gagtaacaat cttattgcaa ccgcagaagt
    23041 aacaaagctt atggcagaac gggatatgta cgtagagaga taatggacgc tcaggatagc
    23101 gcgccacggc ttgtaaaaac gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg
    23161 ggtacgaggt gtatcgtggc ccggaatagt tatatttctg gatcagggcc agatcccagt
    23221 cgatttgctg tacagacatg ctcactcctt ccgatggcgt ctctggcggc tacggcgtac
    23281 cggcggatgc ccgtttcggg tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa
    23341 ccgctgtagt ttaacgaata accacaccag ataacatata accggaaggg ttatcagcag
    23401 aacgggaagg cccacggcgg caatccgtta gttgccccgc agcagacgca tcatgtcttc
    23461 ttgcttctcg tcttcttcct cttcttcgtc atcgtcataa gagaggccga gtttctgcat
    23521 caactcatca atacgatcca gtttagcatc cacccaggat tgctcttcgg cactcagggt
    23581 ttcgcctgct tccagacgtt ccagcagcgc atccagacgc tcatccgttt ccagtaattc
    23641 caactccgcc tgcggtgaaa gcataggttt ctcactcttc ggtttgtgct gtttggtgac
    23701 tttttcagtc acacccaatg gaataggggt tttactgcca atacgtggat ctttcggtgc
    23761 gttctggcct ttgctgcctg acgtgttgtt tccgcccgct gcgcggctgc ccggcgcatg
    23821 accacgacgt tttttctgac gcttgcggtc acgagcttcc tgattcagct cttcgcgcgt
    23881 tttgcgacgc gcttttgcat gacctttgct gcgtgatggt gaagatggtt tcataatggt
    23941 tcgtctttta gccgttttat tcagtataga tttgcggcgg aatctaacag aaagcaagca
    24001 aagaaaaaag gcgacagatt actctgtcgc cttttttcct gactcataac ccttaacggg
    24061 tcttacaatc cttgcaggcc gccaacacac cctgtttttc cattagccgg aacaatccgt
    24121 gttgattcca tttccgttta attacgtctc cggacgtttg tcttcctgac aatcctctgt
    24181 cttcgcctcc tggcgctcct gaccctcatc ctgagtcatt gttcctgtta gcgtcctcgc
    24241 tctatgggtg ttactttacg cctttgcttt aaacaaacaa tcaaccgcca tcagtatttg
    24301 cacattttca gagttttata taaaagcaat tcactgattt atatagctta tttgacatgc
    24361 atcgcagaat tttctctgct atttcgcata aagactatcg gcaatgtctc acaaagcgta
    24421 tggcttttac ttacaacgct cacgccgaaa aatccgcctt ttattatggt tcaggtataa
    24481 tcgccacaaa gctttgactg acggagacga ccgctttgac taatttgaat tatcaacaga
    24541 cgcattttgt gatgagtgcg cctgatattc gccacctacc ttccgatacc ggaattgaag
    24601 tggcttttgc aggccgttcc aacgcaggta aatccagcgc gctgaacacg ctgactaacc
    24661 agaaaagcct ggctcgtacc tcaaaaaccc caggacgcac ccagcttatc aacctgtttg
    24721 aagtggctga cggcaagcgt ctggttgact tgcctgggta cggttatgcg gaagtcccgg
    24781 aagagatgaa gcgcaaatgg cagcgcgcgc tcggcgagta cctcgaaaaa cgtcagagcc
    24841 tacaaggtct ggtagtgcta atggatattc gccatccgct gaaagatttg gatcagcaga
    24901 tgattgagtg ggcggtagac agcaatatcg ccgtgctggt actgctgacc aaagcggaca
    24961 aactggcaag cggcgcacgt aaagcgcaat tgaatatggt gcgtgaagcg gtgctggcgt
    25021 ttaacggtga tgtgcaggtt gaaacgtttt cttcgctgaa gaaacaaggc gtggacaagc
    25081 tgcggcagaa actggatacc tggtttaacg agatgcagcc tgtagaagaa acgcaggacg
    25141 gcgaataatt ttcttacctt aatgcttatg ccggatgtgg cgtgtccggc ccataaattc
    25201 aatagattac aattctaccg ttggcctgat aaacatggtg catcaggcat tgcggatttt
    25261 ttctttcgcc caataaaaaa cgccccagtc attactgact ggggcggcta aaatattcag
    25321 ccaaatccga ttacgtgaag taaaaggtct gaaagataga acatcttacc tctgtaccct
    25381 acgcgactaa ctttactctt ttttgttcag ttcagaaagc actttttgta attttttttc
    25441 agttgttgca taggaatttc taatagccat cacaaaacgt gacagcttat gttgcttact
    25501 tacgaaaaaa gggcatgttc aggcgaatct tagtgcgcct gatcccagtt ttcgccactc
    25561 cccacttcca ccagcaacgg cacatccaga cgggtacagt tttccatcag ttgatgaatc
    25621 tgcttcgcga cggcatcgac atcatctcta tgaacttcaa ataccagttc atcgtgtacc
    25681 tgcatgatca tacgtacacg cggttgctca gcctgtaacc acgcatcaac ggcaatcatc
    25741 gcccgtttga taatgtcggc ggcggttccc tgcattggcg cgttaatggc tgcacgttca
    25801 gccgctgcac gacgagcacc attgctggat ttgatatccg gcagatacag acggcgtccg
    25861 tccagcgttt caacgtagcc ctgctctttc gcctgagcac gggtgcgttc catatacttc
    25921 agcacgccag ggtagcgttc gaagtaaagg tccatgtact tctgcgcttc tttacgtgga
    25981 atgttcaatt gccgcgccag accgaaagca ctcatgccat aaatcagacc aaagttgatc
    26041 gctttcgcgc tacggcgttg ctcgctggtg acggtttcca gtggcaaacc aaacacttct
    26101 gccgccgttg cccggtggat atcttttcct tccgcgaatg cggtcagcaa gcctttgtca
    26161 cgcgagagat gcgccataat gcgcagttca atctgcgagt agtccgctga gacaatcaca
    26221 taatcctctg gcgcaataaa cgcctggcgg atacggcgac cttcttcgtt acgtaccgga
    26281 atgttttgca ggttaggatc ggttgacgat aaacgtcccg ttgcagttac tgcctggtga
    26341 taagaggtat gcacacgccc ggttttcggg ttgatcatca gcggcagctt gtcggtgtag
    26401 gtcgatttca gcttcgccag accacgatac tccagaatca cttttggcaa cggatagtcc
    26461 agcgccagtt cttccagtac ctcttccgac gttgacggcg cgccacccgg cgttttcttc
    26521 agcggtttaa tgccctgttt ttcaaacaga atggtttgta actgcttggt ggaagaaagg
    26581 ttaaattcct cacctgcaat ttcatacgct ttcttttcca gctcagccag acgaagggtg
    26641 agctcttcag aatgattgtg cagcactttc ggatcgatct tcacaccatt acgttcaatg
    26701 cgtgaaagca ccggcaccag cggcatttcg atattctcga agacgttcaa cggccctttg
    26761 tgtttttgca gatccggcca cattttcaga tgcaactgca aggtgacatc tgcatcttcg
    26821 gcggcgtaac gtccggcctc atcgagggca atctggttaa aggtcagttg atttttgcct
    26881 ttacccgcaa tctcttcaaa agtgatggtt ttgtgcttca accaacgttc cgcgaggctg
    26941 tccatatcgt gacgcccggc aacgctattg agaatgtagg actccagcat ggtatcaaac
    27001 gcaatcccac gcagctcaat gccgtaattc gccagaatac cgcgatcgta tttcaggttt
    27061 tgcccgacct tcagcgcctt ttcatcttcc agcagcggtt ttagcaactc gagtgcacgc
    27121 tcgcgagaga tttgatcggg cgcatcaaga taatcatgag caaccggaat atatgccgct
    27181 acgcctgact cgatagcaaa agaaagcccg accaggttag cagagatgtt atcaaggctg
    27241 tcggtttcgg tatcaaatgc aaataccggc gctttttcca gcttcgcaat ccacgctttc
    27301 agtgtttctt catcaaggat ggtgacgtag ttgtcataag aaatcaccgt tgccgtcact
    27361 tctggtgctt cgtctgcaac actggtttcc tgtggcttcg cggcgggttt tgcccctttg
    27421 gcctgtaacc atttgcccgc ttcgacatca gcagtccagc gtttgaactc atactttttg
    27481 aacagcccca acaactcttc cgctgccggt tgctgcactt ccagttgttc acaggtcagc
    27541 tccagttcaa cgtcggtttt aatcgtcgcc agctggtatg agagataagc aacttctttg
    27601 ttttgctcga gcttcgctgc cattgttttc gcgccacgga agctcaaccc agcaattttt
    27661 tctggctcgg catacagcgt atccagcccg ccaagacctt gcagcaatgc ctgcgcggtt
    27721 ttttcaccga cgcccggtac gccaggaatg ttatcagagg agtcacccat cagcgccagg
    27781 aaatcgatga tcagttctgg cggcacgccg tacttattca ccacctcttc cggtccgagg
    27841 atggtattcg tcatggtatt gataagcgta atatttggcg tcaccagctg cgccatatct
    27901 ttatcgccag tgctgatcag caccggacgc ccggcttttt cggcttcgcg cgccagagta
    27961 ccgataacgt cgtccgcttc tacgccagaa accgccagca gcggcagtcc catcgcttta
    28021 accatcgcgt gcaagggttc gatttgtgct cgcagatcgt ccggcattgg cgggcgatgt
    28081 gatttgtaat gttcaaacag ttcatcacga aaggtttttc ccttggcgtc aaagaccacc
    28141 gctgcatgcg tcggtttata ttgcatgatc agactgcgca gcatgttgag gacaccatac
    28201 atcgcaccgg tcggttcgcc tgcgctgtta gtcagcggag gaaacgcgtg atatgcgcga
    28261 taaagataag atgaaccatc tacaaggata agtggatttt gggggatctg aaccataatg
    28321 tccgtgcctg tttatcagat tatgggtaaa ggatgccaca gacaggataa aaacatgagt
    28381 ttttcaccgc ttttcgtgaa aatcttttgt agatcttctg gatcgctcgc aaatcgtcat
    28441 gtggataact ttgtgcataa attatttaca tcgcttttaa tttattatca tgctttttac
    28501 agtatgatgt ttgtctttgt ttttcatgca gttaaaaata aagccatccc taataattga
    28561 gataatggga ttttttctaa tgtggataga tatgaattat ttttctcctt aaggatcatc
    28621 cgttatttgg gtcgttttct gctaaaatct gcgccttgcg cttctttagc gcactgtcta
    28681 tggctaacct tttgaataaa ttcattatga cgagaatact cgctgcgata acccttttgc
    28741 tgagtatcgt attgaccatt ttggtcacta ttttttgctc cgtcccgatc atcattgccg
    28801 ggattgtaaa actattgcta cctgtgccag tcatttggcg aaaggtttcg cgtttttgtg
    28861 attttatgat gtattgctgg tgtgaaggtc tggcggtatt actgcatctg aacccacacc
    28921 tgcaatggga agttcacggg ctggaagggt taagtaagaa gaactggtat ctgcttattt
    28981 gtaaccaccg tagttgggca gatattgtcg tactgtgcgt gctgtttcgt aagcacattc
    29041 cgatgaataa atatttcctc aagcaacagc tggcctgggt gccatttctt ggcctggcgt
    29101 gctgggcgct ggatatgccg tttatgaagc gttattcccg cgcttattta ttacgtcatc
    29161 cggagcgacg tggtaaagat gttgaaacca ctcggcgttc ttgtgaaaag tttcgcctgc
    29221 atcccaccac cattgtcaat ttcgttgaag gctcccgttt cacgcaagaa aaacatcagc
    29281 aaacccactc cacttttcaa aacttgttgc caccaaaggc ggcaggcatt gcgatggcgc
    29341 taaatgtact gggtaaacaa ttcgataaac tgcttaatgt tacgctgtgt tatccggaca
    29401 ataaccgtca gccgttcttc gatatgttaa gcggtaaact gacgcggatt gtcgtacatg
    29461 tggatttaca gcctattgcc gatgagttac atggcgacta catcaatgac aaaagcttta
    29521 agcgccattt tcagcaatgg ctgaactcac tgtggcagga aaaagaccgg cttctgacgt
    29581 cgttgatgtc atcacagcgg catgacaagt aaattttgct cgcctctgaa tgagcagagg
    29641 cgagtgagta ttttaatgaa caaaacgtcc ggcacgagac ataaattctt cttcgctcat
    29701 ctcctgtccg ttaaaaacaa ctttacccgg tgtataatgc agctgcagcg aggcggtatt
    29761 gttgtcaatc gtgattaact gaaacatctg acctaatgtc atcatcccgc tgatttgttt
    29821 atcagcctgc ttttgcgctt tttccgcatc cattccttca gataaattaa gctgttttgc
    29881 ggtttctgtg acgacattaa gcggtaactt cacatcaaaa ttgagcgatt tgatatcttt
    29941 gtttgtggat gatgaagatt tggctgggtc ggcaatactg atatccagat tggcatttag
    30001 ttcgccgagt gcgttcttcc atcttaccgg ttgtttaatg gtcggctcac ttttttgtaa
    30061 taacggtaaa tactctttga acaatgcagc attcatttct tgcagagcaa cttcatcgtt
    30121 tgctaactca gggtgtgcag caagctgctt ctgcatcgca atgttatact ggatgataaa
    30181 ttggcgcact gctgagggat caatagattc agcaatcaat gaccactcac cactaccgag
    30241 ttgctgattc ccttttgtta acttatcaag ttcgtaggtc agattgacat tgatataatc
    30301 tttgtcttta acgcgatcca gcctggtttt tgcaacgaaa gcatcgattt gggcaacgtc
    30361 ttccccctga ttgataatat taacctgatt aaatttcgac tcgctttcac caaccggaaa
    30421 tttcttttct tctaatcgtg tcagagaatc aatggtaaaa ctttttgccg ttactttgtt
    30481 cattggtgat aattgcagaa caatatcttc gactttgcct tcaatatcca ggttagacaa
    30541 tgaactacca tcaccattaa aagtaaactg gccctcaccg aaagaaaact ttccatactc
    30601 tgcagaggca acatctaatg tcgtagagaa tgagccacca aacgcgaatc gcaatgtcgc
    30661 ttccacgggc gatttatttt tcgcggcgat aaatagcggt tgtgtcagtt cattattcac
    30721 taagttcact tttgccgctg ccagtgctgg gatgacatta ccatgcatta acattgtgat
    30781 gggcaacgga ccgtgctcaa catccacatc aaatgcaact ttctggcctg gcttgatatt
    30841 gagatcgggt gcaccgttat caaatgaaat ttgcatttga aaatgggaat taaaaaagcc
    30901 ttt
//
LOCUS       DAZKFU020000030        30627 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZKFU020000030 DAZKFU020000000
VERSION     DAZKFU020000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.030"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            309..1892
                     /gene="yegH"
                     /locus_tag="ACHOQQ_RS05470"
                     /old_locus_tag="pgaptmp_001094"
     CDS             309..1892
                     /gene="yegH"
                     /locus_tag="ACHOQQ_RS05470"
                     /old_locus_tag="pgaptmp_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4469372.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2344..4197)
                     /gene="asmA"
                     /locus_tag="ACHOQQ_RS05475"
                     /old_locus_tag="pgaptmp_001095"
     CDS             complement(2344..4197)
                     /gene="asmA"
                     /locus_tag="ACHOQQ_RS05475"
                     /old_locus_tag="pgaptmp_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGU4469373.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     TAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQILRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4219..4800)
                     /gene="dcd"
                     /locus_tag="ACHOQQ_RS05480"
                     /old_locus_tag="pgaptmp_001096"
     CDS             complement(4219..4800)
                     /gene="dcd"
                     /locus_tag="ACHOQQ_RS05480"
                     /old_locus_tag="pgaptmp_001096"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGU4469374.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4892..5533)
                     /gene="udk"
                     /locus_tag="ACHOQQ_RS05485"
                     /old_locus_tag="pgaptmp_001097"
     CDS             complement(4892..5533)
                     /gene="udk"
                     /locus_tag="ACHOQQ_RS05485"
                     /old_locus_tag="pgaptmp_001097"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGU4469375.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5851..9168
                     /gene="dgcE"
                     /locus_tag="ACHOQQ_RS05490"
                     /old_locus_tag="pgaptmp_001098"
     CDS             5851..9168
                     /gene="dgcE"
                     /locus_tag="ACHOQQ_RS05490"
                     /old_locus_tag="pgaptmp_001098"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4469376.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9277..10125)
                     /gene="alkA"
                     /locus_tag="ACHOQQ_RS05495"
                     /old_locus_tag="pgaptmp_001099"
     CDS             complement(9277..10125)
                     /gene="alkA"
                     /locus_tag="ACHOQQ_RS05495"
                     /old_locus_tag="pgaptmp_001099"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGU4469377.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMSLKRAEALIHLANAALEGSLPMAIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10259..11611
                     /gene="yegD"
                     /locus_tag="ACHOQQ_RS05500"
                     /old_locus_tag="pgaptmp_001100"
     CDS             10259..11611
                     /gene="yegD"
                     /locus_tag="ACHOQQ_RS05500"
                     /old_locus_tag="pgaptmp_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4469378.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQRVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALEEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11624..13564)
                     /gene="yegI"
                     /locus_tag="ACHOQQ_RS05505"
                     /old_locus_tag="pgaptmp_001101"
     CDS             complement(11624..13564)
                     /gene="yegI"
                     /locus_tag="ACHOQQ_RS05505"
                     /old_locus_tag="pgaptmp_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGU4469379.1"
                     /translation="MKPTLYTATGECVTTGRELGKGGEGAVYDINEFADSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPAVQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSAVSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRT
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMELLRQAAEKLAQAQADLSRC"
     gene            complement(13561..14322)
                     /gene="pphC"
                     /locus_tag="ACHOQQ_RS05510"
                     /old_locus_tag="pgaptmp_001102"
     CDS             complement(13561..14322)
                     /gene="pphC"
                     /locus_tag="ACHOQQ_RS05510"
                     /old_locus_tag="pgaptmp_001102"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HGU4469380.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMAGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14319..14978)
                     /gene="yegL"
                     /locus_tag="ACHOQQ_RS05515"
                     /old_locus_tag="pgaptmp_001103"
     CDS             complement(14319..14978)
                     /gene="yegL"
                     /locus_tag="ACHOQQ_RS05515"
                     /old_locus_tag="pgaptmp_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4469381.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15199..15258)
                     /gene="ibsA"
                     /locus_tag="ACHOQQ_RS05520"
                     /old_locus_tag="pgaptmp_001104"
     CDS             complement(15199..15258)
                     /gene="ibsA"
                     /locus_tag="ACHOQQ_RS05520"
                     /old_locus_tag="pgaptmp_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGU4469382.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15531..15587)
                     /locus_tag="ACHOQQ_RS05525"
                     /old_locus_tag="pgaptmp_001105"
     CDS             complement(15531..15587)
                     /locus_tag="ACHOQQ_RS05525"
                     /old_locus_tag="pgaptmp_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4469383.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15859..15915)
                     /locus_tag="ACHOQQ_RS05530"
                     /old_locus_tag="pgaptmp_001106"
     CDS             complement(15859..15915)
                     /locus_tag="ACHOQQ_RS05530"
                     /old_locus_tag="pgaptmp_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4469384.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16194..17441
                     /gene="mdtA"
                     /locus_tag="ACHOQQ_RS05535"
                     /old_locus_tag="pgaptmp_001107"
     CDS             16194..17441
                     /gene="mdtA"
                     /locus_tag="ACHOQQ_RS05535"
                     /old_locus_tag="pgaptmp_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGU4469385.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNEPQSAAPGATKQAQ
                     HPPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATAPEEKATSREYAKKGARS"
     gene            17441..20563
                     /gene="mdtB"
                     /locus_tag="ACHOQQ_RS05540"
                     /old_locus_tag="pgaptmp_001108"
     CDS             17441..20563
                     /gene="mdtB"
                     /locus_tag="ACHOQQ_RS05540"
                     /old_locus_tag="pgaptmp_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGU4469386.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKIFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMLAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20564..23641
                     /gene="mdtC"
                     /locus_tag="ACHOQQ_RS05545"
                     /old_locus_tag="pgaptmp_001109"
     CDS             20564..23641
                     /gene="mdtC"
                     /locus_tag="ACHOQQ_RS05545"
                     /old_locus_tag="pgaptmp_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGU4469387.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23642..25057
                     /gene="mdtD"
                     /locus_tag="ACHOQQ_RS05550"
                     /old_locus_tag="pgaptmp_001110"
     CDS             23642..25057
                     /gene="mdtD"
                     /locus_tag="ACHOQQ_RS05550"
                     /old_locus_tag="pgaptmp_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4469388.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPIGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25054..26457
                     /gene="baeS"
                     /locus_tag="ACHOQQ_RS05555"
                     /old_locus_tag="pgaptmp_001111"
     CDS             25054..26457
                     /gene="baeS"
                     /locus_tag="ACHOQQ_RS05555"
                     /old_locus_tag="pgaptmp_001111"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGU4469389.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26454..27176
                     /gene="baeR"
                     /locus_tag="ACHOQQ_RS05560"
                     /old_locus_tag="pgaptmp_001112"
     CDS             26454..27176
                     /gene="baeR"
                     /locus_tag="ACHOQQ_RS05560"
                     /old_locus_tag="pgaptmp_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGU4469390.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27367..27699
                     /gene="yegP"
                     /locus_tag="ACHOQQ_RS05565"
                     /old_locus_tag="pgaptmp_001113"
     CDS             27367..27699
                     /gene="yegP"
                     /locus_tag="ACHOQQ_RS05565"
                     /old_locus_tag="pgaptmp_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGU4469391.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27897..28194
                     /locus_tag="ACHOQQ_RS05570"
                     /old_locus_tag="pgaptmp_001114"
                     /pseudo
     CDS             27897..28194
                     /locus_tag="ACHOQQ_RS05570"
                     /old_locus_tag="pgaptmp_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
     gene            28196..28492
                     /locus_tag="ACHOQQ_RS05575"
                     /old_locus_tag="pgaptmp_001115"
     CDS             28196..28492
                     /locus_tag="ACHOQQ_RS05575"
                     /old_locus_tag="pgaptmp_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4469392.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28595..29956
                     /gene="yegQ"
                     /locus_tag="ACHOQQ_RS05580"
                     /old_locus_tag="pgaptmp_001116"
     CDS             28595..29956
                     /gene="yegQ"
                     /locus_tag="ACHOQQ_RS05580"
                     /old_locus_tag="pgaptmp_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGU4469393.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(30286..30603)
                     /locus_tag="ACHOQQ_RS05585"
                     /old_locus_tag="pgaptmp_001117"
     CDS             complement(30286..30603)
                     /locus_tag="ACHOQQ_RS05585"
                     /old_locus_tag="pgaptmp_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469394.1"
                     /translation="MKKIVAISLFSIFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CVE"
BASE COUNT         7053 a         8157 c         8086 g         7331 t
ORIGIN      
        1 gttacagcta acggaatagc aggcaaggta acaattcggc aattggctat ttttaagaat
       61 tatattaagt gtcattcaat atggttttta ggagtttctt taggttgaca atatttaata
      121 ttgcgtctcc acatgcgata tttcttaaat aatgttttat tattaccact tttaattcag
      181 ggataatgtg aggttattac cctcaaataa tatgagataa atagtgctgc aacattgcat
      241 ttttgccccg atttatccat gatcgaattg tgacatttgt catacaacga ataggttttg
      301 tacttactat ggaatggatt gccgatccgt ctatctgggc cgggttaatc acgctgattg
      361 tgatcgaact ggtcctcggc attgataacc tggtctttat tgccatcctc gccgaaaaac
      421 taccgccgaa gcagcgcgac cgcgcacggg ttaccgggct gttgctggcg atgttaatgc
      481 gcctgttact gctggcgtca atctcctggc tggtcaccct gactcaaccg ctgttcagct
      541 tccgctcgtt tacctttagc gcccgcgact taatcatgct gtttggtggt ttcttcttgt
      601 tgttcaaagc cacgatggag ctgaacgaac ggctggaagg gaaagacagc aataacccca
      661 cgcaacgcaa aggtgcgaag ttctgggggg tggtgacaca aattgtggta ctggacgcca
      721 tcttctcact cgactcggtg attacagccg tcgggatggt cgaccattta ctggtcatga
      781 tggccgccgt ggttatcgct atcagcctga tgttgatggc cagcaagccg ttaacgcaat
      841 ttgttaacag tcacccgacc atcgttattc tctgcttaag cttcctgttg atgattggct
      901 ttagcctggt ggcagaaggt ttcggcttcg tcattccgaa aggctacctg tacgctgcca
      961 ttggtttctc ggtgatgatc gaggcgctca atcagttggc tatctttaac cggcgacgtt
     1021 ttctttccgc taaccagacg ctgcgccagc ggactaccga agcggtaatg cgcctgctta
     1081 gcgggcaaaa agaagatgcg gaactggacg ccgaaaccgc gtctatgctg gtggatcatg
     1141 gtaaccagca gatctttaat ccgcaggaac ggcggatgat tgagcgggta cttaatctta
     1201 accagcgtac cgtcagcagc attatgacgt cgcgccacga tattgagcat atcgatctca
     1261 acgcgccgga agacgagatc cgccagttgc tggagcgaaa tcagcatacg cggctggtgg
     1321 ttaccgacgg tgatgacgca gaagatttgc tcggtgttgt tcacgttatc gacctgctac
     1381 aacagtcact gcgcggcgaa ccgctcaacc tgcgggtgtt gattcgccag ccgctggtgt
     1441 tcccggaaac cttgccgttg ttacctgctc tggagcagtt ccgtaatgcc cgcacgcact
     1501 ttgcttttgt ggtggatgag tttggttcag tggaagggat tgtgacatta agtgacgtca
     1561 ctgaaaccat tgccggtaac ttaccgaacg aagtggaaga gatcgacgcc cgccatgata
     1621 ttcagaagaa tgcggacggt tcctggacgg cgaatggtca tatgccgctg gaagatctgg
     1681 tgcaatatgt gccgctaccg ctggatgaaa aacgtgaata tcacaccatt gccgggctgt
     1741 tgatggaata tttacagcgt attccaaaac ctggcgaaga agttcaggtg ggggattatt
     1801 tgcttaaaac gttgcaggtg gaaagccatc gcgtgcagaa ggtgcagatt attccgctac
     1861 gtaaggatgg cgagatggag tacgaggtgt gatggtatcg gtcattttac ccgatgctgc
     1921 gtaacgcctt ataacttcag caatgaagcg ttgtctgtgg tacatggaca ttacagcggg
     1981 ggtgtgggtc cggctgtaaa tcgccgaatc gtttcctgta ggccggggca gcccacatgg
     2041 atgtgggctg agggcgcgtt ttacagggat gttacctcgc gcccgtaccc gatagccgaa
     2101 aggaataaga tgagggaacc gcgccagcgg cgatttaccg ccgggagccc gggttgccag
     2161 gatggtggcg attgagccac cctggcacgt tcaccggctt gagcgttcca tagtagtgag
     2221 gaacatcaag tgaacggaac agccttcgat cttacatatt cagaatataa tggtgccgcc
     2281 gcgaacgcct gatgcgacgc tttacgcgtc ttatcaggcc tacaggacag gtcacctggt
     2341 aacctacatc ttctccagca acttcttcac atctttgcca ttgcgggaat ctttattccg
     2401 ctcggcccag tcattcaggc gacgtttcgc ttcgtcctgt agatgtttgc gcagtatctg
     2461 atccacttgc agactgtaat tgagttgctg ccagttgcca taaacccgca gcggtactgg
     2521 cgtttctttg aggaaatcaa tcagtttgct ttccccgttc cagccaccca cgacccgaat
     2581 atcaaactgg gtgtcgcagg tttgatctgc cagattcaac atgccttccc ctgtaagcgc
     2641 cagcacaggc gattgacctt gcatgtcgtt taacgtcacg acgccatcct tcaacgtcaa
     2701 atcggtggta aagcggtcaa gacgcgtcac gttatcgaag ttttcagcgg ccttcacatc
     2761 accaccatta cgttctaccg cttgctgaat catctgctgg aagttcatcc cttccatgcg
     2821 cgtatcggcc atttcgacat gcgccgtccc ctgccagttg tggcggaatg cgtcggcatc
     2881 tatgtcagca ccggagaaat caccagccag tgacatcttt ccggtcaacg aaatcggata
     2941 gttaaacgcc ttcagaatgg tgccaatctc aacgttttcc agccgtggct ggaagtttat
     3001 ccgcggattt attgatgtcg cgtccagcgt gcccggcagt gaaacctgtc caccgttaag
     3061 tttgccctgc agttgagtaa tttccagcaa acccgacttg ttggtcattt gcgtggcaac
     3121 atctgtaaaa ttcattccgc gccagcgcac gttactggcc tgcaacaaaa tatcagccgt
     3181 aaagccttgc agtccctgat aggccggttc atcaatacgc gaagaaatga ccgggcgcgg
     3241 caacgttgat tggctctgcc cctgctgcgc ggcaccgttt tcaccattcg cggtttcatt
     3301 aagcgggatg aggttgtcaa gattcagttg cgggaattgc agtctcagct gccattccgg
     3361 tttctctgtc agcgtgacct gtgcttgccc gctcagcgta ctatcattgg cggtcaaact
     3421 aatttggtta aatgaaaggc gtttatgtga ctcctgccac tgggcctgga aactcccctg
     3481 cccctgaata ccttgttttg gtaaatctgc accctgcaac tgccagttaa tttgttcaat
     3541 agccgccgtt aaatcatgcg gataatcaga agcatctacc gtaccgttaa gggatatcgt
     3601 gagatcgcgc tgatcgcgat taacccgccc ggaaaactca aatgagccac gatgttgggg
     3661 atcttgttcc atttgcaggc gaatattgcg gattgtcacc tgctcgtcat cttcatgctg
     3721 gaacaccaga acgctgtccg ccaccttaag actggatata tcaaacgacc atccgcgatc
     3781 gtctgacaga tccggcaagg tattgtcgcg cggtgcaacc ggagcgtctt cactgcgcac
     3841 cgcttccgtc tgcggcgtca gttggatcac tgcccctttt agcatcacct gcttaacgct
     3901 cagttgatga ctcagtagtg gtaaaagcgc cacgtccagg cgcatgttgt cggcgcgaac
     3961 cagtggctgg ctcgcgccct gggcggtgag agacattcgc ccggagagga tactaagctg
     4021 cggccagacg tgccaacgca gtggcccgtc gagctgcaat tgataaccgc tacgtgcagc
     4081 aacttgcttg accatatagt cgcggaaatc attcggattc accagcaaca ctaacgcaga
     4141 taacccggcc accagcacga ccaggagtat catcagcgtc gtcagaaatc gtctcatggt
     4201 atcctcaatg ggcctgaatt agtctttatc gattcggctg gctaccgcgc cctgctggtt
     4261 gcgatatttc gcatcttcac ggcggttgta aggtcgcgcc gccgggccgg aaagcggctc
     4321 aaagctcagc gcaccaatta acatgcccgg acgcagcgcc agcggcagct taccggagtt
     4381 gtagaactcc agcacaatgc agccagacca gcccggatcg atgcggtgcg cggtgacgtg
     4441 caccatcagc cccagacgcg ccaaagagga acgcccgtcc agccagccca ccagatcggc
     4501 tggcagcgtc accgactcca gcgtcaccgc cagcgccagc tctcctgggt gaagatagaa
     4561 cgcctcgctt tcatcgagaa cgatctcatc gctcatcacg cggtcaagcg cggcgctcac
     4621 ttcatctttg ggaccgctca gatcgataaa cgctgccgtg tgaccacgga aggtacgaaa
     4681 tttattgccc aggcgtacat ccaccgtcgc gccgttaata cgctccactg gcggacgtgg
     4741 gttgatcgac aaacggcctt catcaagcca ggcttcaata tctcggtcac acagacgcat
     4801 ggcattttct cctttcgcgc atcactccct taacgccaat tacgtcaagg gcataccagg
     4861 ttatcactga acggtacaca atttatcaag cttattcaaa gaactgactt attttcgctt
     4921 tcaatatatc gatcgcgatg cggtttttcc cgccgcgcgg cacgataatg tccgcatatt
     4981 gtttagaagg ctcaatgaat tgcaggaaca tcgggcgcac ggttttttga tattgcgcca
     5041 tcacggaatc cattgaacgc ccacgctcgt taacgtcacg cttgatgcgg cgcatcaggc
     5101 agatatccag cggggtatca acgaaaatgg agaagttaag ttcgtcacgc aaacgcgcat
     5161 ccgtcagcaa caaaatgcct tcgagaatga tgaccttctt cggctcaacc gtcaccgttt
     5221 ctttcatacg cgtatgttca acatagctgt aaaccggcag gtcaattgcc gagccgcgtt
     5281 tcaacgcttg taaatgctca agcagcaggc tgtgatccat cgcgctggga tggtcgtagt
     5341 tggtcttaac gcgttcttcc atcgacagat ggctttgatc tttgtaatag cagtcttcgg
     5401 gaattacgcc gatgtgttca tcaccgactt gctcacgtaa ttcacgataa agggtactgg
     5461 caataagact cttgccggaa gccgatgcgc cagcgatacc gataatgacg cactgatgag
     5521 actgatcagt catatattta gcgacctgat taacctggat gttaggaagg gggcgacgaa
     5581 gcgccaaacg cggcaattat agggatttca tccgcctgat accagtcgaa tagcgttgcc
     5641 gcgcgctcag agttaattgt tgacaaagaa ttcccggtgg caaattacgt tgatcagttt
     5701 tatacaaagt aaaaaatgtt atacgcagtt gcgcaaatta tccgccttta cgtcacttta
     5761 tgagcaattc gcatataaaa tgtaaaactt ttgtactagc ataaacacag aaacgaatac
     5821 tggcgacctg gccttgcgga taaagcggta atgagcaaac aatcacagca tgtattaatt
     5881 gccctgcccc acccgctgct tcacctggtc agtttaggtt tagtctcgtt tatctttacc
     5941 cttttctcgc ttgagctttc gcagtttggc acccaactcg ccccactgtg gttcccgacg
     6001 tccatcatga tggtggcgtt ttatcgccat gccgggcgca tgtggccggg aattgcgctg
     6061 agctgttcgc tgggaaatat cgccgcatcc atcctgcttt tttccaccag ctcgctgaac
     6121 atgacctgga cgaccatcaa tattgttgaa gccgtggtcg gggcagtgct gctacgtaaa
     6181 ttgctgccgt ggtataaccc cttgcaaaat ctggctgact ggctgcgtct ggcactcggc
     6241 agcgccattg ttccgcctct gttggggggt gttttggttg tcctgctgac gcccggagac
     6301 gatcctctca gggcattttt gatatgggta ctgtcagaat ccatcggcgc tctggcactg
     6361 gtgccgctgg gattgttatt taaaccacac tatctgctgc gccatcgcaa cccaaggttg
     6421 ctttttgagt cgctgctcac gttagccatc acactgacgt taagctggct ttcgatgctg
     6481 tatctgccgt ggccttttac tttcattatt gtgctgttga tgtggagcgc cgtgcgcctg
     6541 ccacgaatgg aagccttttt gatcttcctt accacggtga tgatggtgtc gctgatgatg
     6601 gccgcggatc cctccctgct tgctacgccg cgtacgtacc tgatgagcca tatgccgtgg
     6661 ctaccgtttt tgctgatcct gctgcccgcc aacatcatga cgatggtgat gtatgccttt
     6721 cgtgcggaac gcaaacacat ttccgaaagc gaaacccgct ttcggaacgc gatggaatat
     6781 tccgctatcg gtatggcgtt agtgggcacc gagggacaat ggctgcaatc caacaaagca
     6841 ctctgccagt ttctcggtta cagtcaggaa gagctgcgcg gactcacctt tcagcaactg
     6901 acctggccgg aggatctcaa taaagatctc caacaggttg aaaagctgat aagcggtgaa
     6961 ataaacacct attcaatgga aaaacgttac tacaaccgca atggcgatgt tgtctgggcg
     7021 ttgcttgccg tctctctggt gcgccacacg gatggcacgc cgctctattt tatcgctcag
     7081 attgaagaca ttaacgagct aaaacgcacc gaacaggtga atcagcaact gatggagcgc
     7141 atcacgctgg ctaacgaagc gggcgggatt ggcatctggg agtgggagct gaagccgaat
     7201 atttttagct gggataagcg gatgttcgaa ctgtatgaaa ttcctccgca tatcaaaccg
     7261 aactggcagg tgtggtacga gtgcgtgctg ccggaagatc gtcaacacgc cgaaaaagtg
     7321 attcgtgatt cgttgcaatc acgctcgccc tttaagctgg aatttcgcat tgccgtgaaa
     7381 gatggcatcc gccatatccg cgccctcgcc aaccgggtac tgaataaaga aggcgaagtc
     7441 gaacgcctgc tcggcattaa tatggatatg accgaggtta aacagcttaa cgaggcattg
     7501 tttcaggaaa aagagcgcct gcacattacg ctggactcca ttggcgaagc cgtagtctgt
     7561 attgatatgg cgatgaaaat tacctttatg aatccggtgg cggagaagat gagcggctgg
     7621 acgcaggaag aagcattagg tgttccgctc ctgacggtgt tgcatattac ttttggcgac
     7681 aacgggccat taatggagaa tatttacagt gccgacacct cacgttccgc gatcgaacaa
     7741 gatgtggtgt tgcactgtcg gagcggcggc agctacgacg tgcattacag tattacgccg
     7801 ttaagtactc tggacggcag caatattggt tcggttctgg tgattcagga cgttaccgaa
     7861 tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact gacgcatctc
     7921 gccaaccgcg ccagttttga gaaacaactg cgtatcctgc tgcaaacggt aaacagtacg
     7981 catcagcgac atgcactggt gtttatcgat cttgatcgct ttaaagcggt gaatgacagc
     8041 gccgggcatg cggcgggcga cgctttgctg cgcgaactgg cgtcgttgat gctgagtatg
     8101 ctgcgctcca gtgacgtgct ggcgcggctc ggcggtgatg aatttggtct gctgctgcca
     8161 gactgcaatg ttgaaagcgc tcgttttatc gctacacgta ttatcagtgc tgtgaatgac
     8221 tatcacttta tctgggaagg ccgggtacat cgggtaggtg ccagcgccgg gattaccttg
     8281 atcgatgaca acaatcatca ggcggctgaa gtgatgtcgc aggctgatat cgcctgttat
     8341 gcctccaaaa atggtggccg gggccgggtg acggtttacg aaccgcagca agctgccgca
     8401 catagcgaac gggcggcgat gtcgcttgat gaacagtggc ggatgattaa agagaatcag
     8461 ttgatgatga tcgcccacgg tgtcgcttcg ccacggatcc cggaagcgcg taatttgtgg
     8521 ctgatttcac ttaagctctg gagttgcgaa ggcgagatta ttgatgaaca aacatttcgt
     8581 cgtagcttca gcgatccggc gcttagccat gctcttgacc gccgggtatt ccacgaattt
     8641 ttccagcagg ccgcaaaagc ggttgccagt aaaggcataa gcatcgctct ccccctttcc
     8701 gttgccggtt tgagtagcgc cacgctggtg aatgatctgc ttgagcagct ggaaaatagc
     8761 cctctaccac cacggttatt acatctgatt attccggctg aagcgatttt agatcacgca
     8821 gaaagcgtgc aaaaactgcg gctggcggga tgtcggatag tgctcagcca ggtgggccgc
     8881 gatctgcaaa tcttcaactc gctgaaagcg aatatggcag attacctgct acttgatggt
     8941 gagttatgcg ccaacgtgca gggtaatttg atggatgaga tgctgattac gattattcag
     9001 gggcacgctc agcgactcgg gatgaaaacc atcgccgggc cagtcgtttt acccttagtg
     9061 atggatacgc tttctggcat cggcgtcgat ctgatttatg gtgatgtgat tgccgatgcc
     9121 caaccgctgg atttgctggt gaatagtagt tatttcgcga ttaactgagg cgtgcttccc
     9181 catcgcctga tgcgacgcta acgcgtctta tcatgcctac aaatcgctca ttccccaggc
     9241 cggataaggt gctcgcaccg catccggcga ccaacgtcat gcttcgtctg gttgccagcc
     9301 ttccgtatac cagatatgca acagcgcata agaacgccag ggcttccagc gttcggcata
     9361 acggcggatt tgtgccggtg tcattcccgg aaatcgctgt ttaatcagat aatcatccgg
     9421 caggaaaaca tctttcgcct gccagccacg caaagcgaaa taattcgccg tccagcgccc
     9481 gatacccgga aaagtttgca gcgttttcat cgcctgctcc acatcgcccg gaattgccat
     9541 tggtaagctg ccctccagcg ccgcatttgc cagatgaatc agtgcctctg cccgtttcaa
     9601 cgacatgcct aacgctttta atgcctgcgg gtcggctgct gccagccgct gaggcgtggg
     9661 gaagcagata tactccggaa aatcatccag ccgttcgcca taaagctgta ccactttggc
     9721 agtcaatttt gccgccatcg ccacgctcac cagttggcct aaaatcgccc gcacgccctg
     9781 ctcaaaagca tcaacactgc cgggtaaacg caatccgggc cgcgccgcgc ctaacttgcc
     9841 caacgcaccg ttaacaattt gtgggttaca ttgcagatca aacaggcggc tcattttcgc
     9901 cagacactct gcggcaacag gttctaaacc tgcacttaaa tttatgtgca gagtatggcg
     9961 ggctatatcc ggaatagcag tcactacgcc gcgatattcg cccaccgcca gactacgggc
    10021 ataataactg tccgcgaccg tttccacacc gctcaccgca cgggcggcga gaaatcccaa
    10081 catccacgac cagtcatacg gcggctgcca gttcagggta tacatcgcat ctccttattc
    10141 atccgctttc agcataaacg ttattcagac gctgcgcttt gctttcatat tccggttgtc
    10201 gcgacggcaa catttcgcta aagtcacgcc ccttcttcac cggcatgggg attatttcgt
    10261 gtttattggt tttgattacg gtacagcaaa ctgttcagtg gcggtcatgc gtgacggtaa
    10321 accgcacttg ctaaaaatgg aaaacgacag cacgctgctg ccttcaatgc tttgcgcgcc
    10381 aacgcgtgag gcggtaagcg aatggctgta ccgccatcat gatgttccgg cagacgacga
    10441 tgaaacgcag gcgctgttgc gtcgggcgat tcgttataac cgcgaagaag atatcgatgt
    10501 tacggcgaaa agcgtgcagt tcggtctttc ctcactggca cagtacattg atgatccgga
    10561 agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt gccagcggct taaaaccgca
    10621 gcgggtagcg ctgtttgagg atctggtctg cgcaatgatg ttgcacattc gccagcaggc
    10681 gcaggcgcag ctgccagaag cgattactca ggcggtgatt ggtcgcccga tcaacttcca
    10741 ggggctgggc ggcgatgaag caaacgccca ggcgcaaggg attctggaac gcgcggcgaa
    10801 gcgtgccgga ttcagggacg tggtattcca gtacgagccg gtagcggctg ggctggatta
    10861 cgaagccacc ttacaggaag aaaaacgggt actggtggtg gatatcggcg gtggtacgac
    10921 tgactgttca ttgctgctga tggggccgca gtggcgttcg cgtctcgatc gtgaagccag
    10981 cctgctgggt cacagtggtt gccgtattgg cggtaacgat ctggatatcg cactggcgtt
    11041 taaaaacctg atgccgttac ttggcatggg tggcgaaacc gaaaaaggca tcgccctgcc
    11101 gattctgccg tggtggaatg cggttgccat caacgacgtt cctgcgcaga gtgatttcta
    11161 cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc gatgcccgcg aaccggaaaa
    11221 agtggccctg ttacagaaag tctggcgtca gcgtttaagc tatcgcctgg tgcgtagcgc
    11281 agaagagtgc aaaattgctc tttcaagcgt agcggaaacc cgcgcctcac tgccgtttat
    11341 cagcgatgaa ctggctacgc tgattagcca gcaagggctg gaaagcgccc tcagccagcc
    11401 actggcgcgg attctggaac aggtgcaact ggccctcgat aacgcccagg aaaaaccgga
    11461 cgttatctat ctgaccggcg gtagcgcccg ttctccgctg attaaaaaag cgctggaaga
    11521 acagttgccg ggcattccga ttgcaggcgg cgatgacttt ggctccgtca ccgccgggct
    11581 ggcacgctgg gcggaagtgg tgtttcgtta attgaaaaat ggctcagcag cggcttaaat
    11641 ctgcctgcgc ctgagccagt ttttctgccg cctgacgtaa cagttccatc aacggcatgg
    11701 tccgtgctgg agcatgaagg cggaatcgct gcaactctgt cgcgccgaca gtcaacgccg
    11761 attccagccg atgtcgtttg gcggccattt tcgccattac cgcttgtctg tctgcaggcg
    11821 ttaccgcttc gttcggcctg aacacaaaac ggcgttcaca gctcgctttc cagtcgatga
    11881 ccgcctgggt cagatgatca ccaaaccctt taacttgctt aaccccacga cgggtaacat
    11941 ccgctgctgt ttcaatacca aaggaccgta acgccgcttt acgcgcaggg ccaacaccgg
    12001 gaatagaggc aacatcaata aaaaatccct ccagaaactt ctgcttctgc cgttcccttg
    12061 cagtgtcgtg aagtgctgcc agagcacgtt tttcttcttc cggtaacccg agcatttcgt
    12121 ccttcatttt ttcgagcatc gtccgtttgg caataaaacc ttccagccca cccaactgtt
    12181 ggatctggct gactaaatgg tcatagtcca ttttcgtgcg attaaatgcc tcacggcgtt
    12241 gctggatttc tgctttataa gctttgcttg tcagactgcc aataatccag atagccgcca
    12301 gcacaggaac caaaataata taacgcggtt ctgcctgaag gccgcaaagc agcagcgata
    12361 ccgctgacag tgcgatctca agcagaatga tgtattcgcg ccgtaacagg cctaaaggaa
    12421 gcggcctgcc agtcggttgg aaatgatcgg gtaatggcaa ttgcactgct ggcggtgcta
    12481 ctgacgccat caccatcgcc cagactttcg ccagcacaaa atccccgccg gtggtaataa
    12541 cctcttcgcc gagatcaata aaatagataa cgccttgatt atccagcgta caccacgggc
    12601 agtcagtcaa atgagcgggg taaacatgca ttgctgaaac ggtacatttc tttaactgtt
    12661 ggcgtagaga gtccagtgcc gctacccacg cttttgccgt cggacgcccc gttgccacgc
    12721 cactttccgt gaatgcctgc tgaaacatgg cttcaacatc tcccggtaac atcgacagcg
    12781 gaatagatcg tggcggcggt tttaaaccac gtcgctgatt atctgacgcg taggcataac
    12841 ggaaatgggc aatatccgtc tccagcgcat tacccgcatc agagataagc ggcacaccag
    12901 aataaggatg ccgcccacca aacaagacgt gaaaaatcag caacgcaagg ccaaaattat
    12961 cgtgattcgc ggtacgttca aaaccgacaa atgatggcaa ggtttgtagc tctggcggcg
    13021 taaaatgcga cacgccgact tcgcataaat gcagtgtgcc attggcgtta atctgaaagg
    13081 agtcactatc gatcaacacc actttgctgt cgcgacctac cataaagctg ttctggttta
    13141 cgtcacccac gacgtgcccg tgctcgtgaa ccgtagcaaa agatgaagca atattgcgcg
    13201 caacatagag tagaaaatcc cacgcacaat gagggtaact ctggcgacga tgtgccgggc
    13261 tatagatcat atgaatcggt tctttaccag aaacttttgg catcataaaa ccgataactt
    13321 ttccgcctcg tccaccgtga agcgttgcct gcggccaggc gacataattc aacaactgcg
    13381 cgtcagctgt cgcagccata aaggcaagtt tgtcctgttt taaggcgggt ggcggcgtgt
    13441 gataaatctt ggcgacgcta tcggcaaact cattgatatc ataaaccgca ccttcaccgc
    13501 ctttgcccag ttcacggcct gtcgtaacgc actcaccagt agcagtatat aaagtgggtt
    13561 tcatggtaac cacagcgcca gtgccaacgt tttatcatca tcggtacgct cgtttaccgc
    13621 cgggctggac aaaaactgct ttaacaattc aggtaataaa tcgagctgtt cctgcgttgc
    13681 tgccgccagt ccattgaaaa aaggggtaaa gaaaggcaca tgcggagaat tatccaacat
    13741 atttaacgcc agtcgctgga taccatccgt aaatgctgca actttatgca cacgctcagt
    13801 gctggtgaaa gtttccagtc tggaaacggc atcttcatcg gtaatgaaat gcgtcatatt
    13861 ggcatattca cccgccatcg gcgtaagcgg taattgcaga ccgtgaccaa aatccaccac
    13921 cacgccgcca tcgccaatct gcataatcaa ggtgccgtta gccgatgaaa tcaatcccag
    13981 aaacgtgcag gcaaaatcgc gaactgctaa ttctttggct tccgcctcgg caaaaagtcg
    14041 ctgacgaacg gtcagcacaa tatccgtggc gaggatatca ttgagtccaa attcaccgtg
    14101 ctgcactttt tgcgacacat aagccatcgc ttcattgatg gcaagcatcg cgccttcgcc
    14161 accctgcgag acactgcctg cgccatcagc gacaaatacc gacaataatg gctgctggtc
    14221 atttagccag gcaacctgca tctggcaggc atcctggcag ggtaaatcag cgctaatgtg
    14281 cgacgttcca acagcggatg cataaaccag acgccagctc acactgacgt ccagcctttt
    14341 ggcgcttcca gtacaacttc cgttcccggc gtggagcggg aaaccgaacg cagtgaactg
    14401 gataaccagc taaacagttc gcggaattgt aatccctgca gagacaaagg ctgacgaacg
    14461 ctgatttgtg ccagagtctt catatccgcc ccctgaacac cgatggaaaa gaaggcaaac
    14521 ttcttatctt cttccccctg aaacactttg ttggcggcag cctgccactc atcggttgga
    14581 gcgccatcgg tgatcaggaa aatccacggg cgataatagg aaataccatt tgcgcggtac
    14641 tcacgtttcc gctcctctac catatccagg gctttggtaa tggcggcacc cattggtgta
    14701 tcgccctggg caaacaggat gggcgggaaa aaattagcgg cgctggtaaa gggttgttcc
    14761 acatgcaccg ggccgaacgt cacaatacca agttccactc tttttaaggc cagcgggtcg
    14821 gcaagcagct catcgcgaaa ggtaaccaat ccggcgttaa gttcattaat gggtctgcca
    14881 ctcatcgaac cagaaacatc cagcagcaga atgcaggggc aacgtggttc cgggttactg
    14941 gcaaaatcgc tggtggcaaa cgtgatttgt tcgctcataa gaaatccttt cttagagaat
    15001 tattgaggtc cggtatcata ctgattgcaa taatgaaata agagcaaacg aagaaacttt
    15061 cagaattagt cccggctgcg cagaaaattt tgctattcgc ctcgcaagaa cggagagccg
    15121 tcgattgaca ctaagggcgg agtgacataa tttcaggagt gagggttagg gagaggtttc
    15181 cccctccccc tggtgttctt agtaagcctg gaagctaatc actaagagta tcaccagtat
    15241 gatgacgtgc ttcatcataa ccctttcctt attaaaagcc ctcttctccg ggagagggtt
    15301 tcccgtttca gcgtcccgct gaaatcgtcg gcttacctcc tttcgccctg aatgcagtct
    15361 atcgctaacg cgtgaacaaa atagttccct ctgtgattat tgcgtaacga ctcgcaaaga
    15421 atatttgctg gcaggatcgc agactgcaaa gcctgcagat tgacaatctt atcgtgaagg
    15481 catactttca ggagtaaggg tagagcgggg tttcccccgc cctggtagtc ttagtaagcc
    15541 gggaagctta tgactaagag caccacgatg atgatccact tcatcatgac cctttcctta
    15601 tttaaggccc cttcctcggg aggggctttc ccgtttcagc gtcccgctga aatcgtcggc
    15661 ttacctcctt tcgccctgaa ttcagtctat cgctaacgcg tgaacaaaat agtttcctct
    15721 gtgattattg cgtaacgtct cgcaaagaat atttgctggc aggatcgcag actgcaaagc
    15781 ctgcggattg acattctgaa cgtgaaggca tactttcagg agtgagggta gagcggggtt
    15841 tcccccgccc tggtagtttt agtatgctgg gtagctaatc actaagagca ccacgatgat
    15901 gagtatcttc atcatgaccc tttccttatt taagacccct tcctcgggag gggctttccc
    15961 gtttcagcat cccgctgaaa tcgtcggctt acctcctttc gccatgcatg cagtctatcg
    16021 ctaacgcgta gataaaatag tttcctgtgt tattactgga tgcgtgctcg caaatgtgcc
    16081 cgccattcag acgattcccg acagtgtttc ataattcctc catttttctc ccttattggc
    16141 tggctacact agtatcattc cgcgaaacgt ttcaggaaga gaaactctta acgatgaaag
    16201 gcagttataa atcccgttgg gtaatcgtaa tcgtggtggt tatcgccgcc atcgccgcat
    16261 tctggttctg gcaaggccgc aatgaacccc agagcgcagc accaggtgcg acgaaacaag
    16321 cgcagcaccc acctgcgggg gggcgccgtg gtatgcgttc tggcccttta gcgcctgttc
    16381 aggcagcgac tgccgtggaa caggcggttc cgcgttatct caccgggctt ggcaccatca
    16441 ccgcggctaa caccgttacg gtgcgcagtc gcgtggacgg ccaactgatg gcgttgcatt
    16501 tccaggaagg ccagcaggtc aaagcaggcg atttactggc agaaattgac cccagccagt
    16561 ttaaagttgc attagcacaa gcccaggggc aactggcaaa agataaagcc acgcttgcca
    16621 atgcccgccg tgatctggcg cgttatcagc aactggcaaa aaccaatctc gtatctcgtc
    16681 aggaactgga tgcccaacag gcgctggtca gtgaaaccga aggcaccatt aaagccgatg
    16741 aagcgagcgt cgccagcgca caactgcaac tcgactggag ccggattact gcgccagtcg
    16801 atggtcgtgt tggcctgaag caggttgatg ttggtaacca aatctccagt ggcgacacga
    16861 ccgggattgt tgtgatcacc cagacgcatc ctatcgattt gctctttact ctgccggaaa
    16921 gcgatatcgc taccgtagtg caggcgcaaa aagccggaaa accgctggtg gtagaagcct
    16981 gggatcgcac caactcgaag aaattaagcg aaggcacgct gttaagtctg gataaccaaa
    17041 tcgatgccac taccggtacg attaaagtga aagcacgctt taataatcag gatgatgcgc
    17101 tgtttcccaa tcagtttgtt aacgcgcgca tgttagtcga caccgaacaa aacgccgtgg
    17161 tgatccccac cgccgccctg caaatgggca acgaaggcca ttttgtctgg gtactgaata
    17221 gcgaaaacaa agtcagcaaa cacctggtga cgcctggcat tcaggacagc cagaaagtgg
    17281 tgatccgcgc aggtatttct gcgggcgatc gcgtggtgac agacggcatt gatcgcctga
    17341 ccgaaggggc gaaagtggaa gtggtagaag cccagagcgc caccgcaccg gaagagaaag
    17401 ccaccagccg cgaatacgcg aaaaaaggag cacgctcctg atgcaggtgt tacccccgag
    17461 cagcacaggc ggcccgtcgc gcctgtttat tatgcgtcct gtggccacca cgctgctgat
    17521 ggtggcgatc ttactcgccg ggattatcgg ttatcgcgcc ctgcccgttt cggcgctgcc
    17581 ggaagtggac tatccgacca ttcaggtggt cacgctctac ccaggtgcga gcccggatgt
    17641 catgacctct gccgttaccg cgccgctcga acgccagttc gggcagatgt ctggtctgaa
    17701 acagatgtcg tcgcaaagtt ccggcggtgc gtcagttatc actttgcagt tccagctaac
    17761 attgccgctg gatgtcgccg agcaggaagt tcaggccgcg attaacgccg ccactaacct
    17821 gctacctagc gatctgccta acccgccggt ttacagcaaa gtgaacccgg cagatccgcc
    17881 gattatgacg ctcgccgtca cctcaaccgc catgccgatg acccaggtag aagatatggt
    17941 ggaaacccgc gtcgcgcaga agatctcgca gatttccggc gtcggcctgg tgacactttc
    18001 cggcggccag cgtccggctg ttcgcgtcaa acttaacgct caggcgattg ccgccctcgg
    18061 cctgaccagc gaaaccgtgc gcaccgccat taccggtgct aacgttaact cggcaaaagg
    18121 tagcctcgac ggcccttccc gcgcggtcac gctttccgcg aatgaccaga tgcaatccgc
    18181 cgaagagtat cgccagttga ttatcgccta ccagaacggc gcgccgattc gtctgggcga
    18241 tgtcgcaacc gtagagcaag gggcagaaaa cagctggctc ggcgcgtggg cgaacaaaga
    18301 acaggccatt gtgatgaatg ttcagcgcca gcccggtgct aacattatct ccaccgccga
    18361 cagcatccgg cagatgctgc cgcagcttac cgagagtctg ccgaaatcgg tgaaggtgac
    18421 ggtgctttct gaccgcacca ccaatatccg agcatccgtc gacgataccc agtttgaatt
    18481 gatgatggct atcgcgctgg tagtcatgat tatctacctg tttttgcgca atattccggc
    18541 gaccatcatt cccggtgtcg ctgtgccgct gtcattaatc ggcaccttcg cggttatggt
    18601 gtttctcgat ttttcaatca ataacctgac gctgatggcg ttaactatcg ccaccgggtt
    18661 cgtggtcgat gacgccatcg tagttatcga aaatatttcc cgctatatcg aaaaaggcga
    18721 aaaaccgttg gcggcggcgc tcaaaggcgc gggtgaaatc ggctttacca ttatctcgct
    18781 gaccttctca ctgattgcgg tgttgatccc actgctgttt atgggcgata tcgtcgggcg
    18841 actgttccgc gaatttgcta ttaccctggc ggtagcgatt ttaatctcag ccgtggtgtc
    18901 gctaaccctg acaccgatga tgtgcgcacg gatgctcagc caggagtcgt tgcgtaaaca
    18961 gaaccgcttc tcccgtgcct cggaaaaaat attcgacagg ataatcgccg cctatggtcg
    19021 tggactggcg aaagtgctga atcatccgtg gctgacctta agcgtggcgc tcagcacgct
    19081 gctgcttagc gttctattgt gggtgttcat tccgaaaggt ttcttcccgg tacaggataa
    19141 cggcattatt cagggcactt tgcaggcacc gcaatccagc tcctttgcca atatggccca
    19201 gcggcaacgc caggtcgcgg acgtgatttt gcaggatccg gcagtgcaaa gtctgacctc
    19261 atttgttggc gttgatggca ctaacccgtc gctgaacagt gcgcgtttgc agatcaacct
    19321 caaaccgttg gatgaacgtg atgatcgggt gcaaaaagtc atcgcccgtc tgcaaacggc
    19381 ggtggataaa gtgccgggcg tcgatctctt cctgcaacca acgcaggacc tgaccatcga
    19441 tactcaggtc agccgcaccc agtaccagtt taccttgcag gccacgtcac tggatgcgct
    19501 cagtacctgg gtgccacagt tgatggaaaa actccagcag ctgccacagc tttctgatgt
    19561 ctccagcgac tggcaggaca aagggctggt ggcgtatgtc aatgttgatc gcgacagcgc
    19621 cagccgtctg gggatcagca tggcggacgt cgataacgcc ctgtacaacg cgtttggtca
    19681 gcggctgatt tccactattt atactcaggc taaccagtat cgcgtggtgc tggagcacaa
    19741 caccgaaaat accccaggcc tcgcggcgct ggataccatt cgcctgacca gcagcgacgg
    19801 cggcgtggtg ccgctaagct caattgccaa aattgagcag cgttttgcgc cgctttccat
    19861 caaccatctg gatcagttcc cggtaacgac catctccttt aacgtgccgg ataactattc
    19921 gctgggcgat gcggtgcagg caattatgga caccgagaag acgctgaatc tcccggtgga
    19981 tatcaccacg caattccagg gcagcaccct cgccttccag tcggcgctgg gcagcactgt
    20041 ctggctgatt gtcgcggcgg tggtggcgat gtatatcgtg ctcggcattc tgtacgagag
    20101 ctttattcac ccgatcacca ttctctcgac gctacccacc gcaggggtcg gcgcactgct
    20161 ggcattaatg cttgccggaa gtgagctgga tgtgattgcg attatcggca ttattttgct
    20221 gatcggtatc gtgaagaaga acgccatcat gatgatcgac ttcgcgctgg ctgctgagcg
    20281 cgagcaaggc atgtcgccgc gcgatgcaat ctaccaggct tgtctgttgc gttttcgtcc
    20341 gatcctgatg accaccctgg cggctctgct tggcgcgctg ccgctgatgt tgagtaccgg
    20401 ggtcggcgcg gaactgcgtc gtccgttagg tatcggcatg gtcggcggcc tgattgtcag
    20461 ccaggtgctg acgctgttca ccacgccggt gatttatctg ctgtttgacc gcctggcatt
    20521 gtggaccaaa agtcgctttg ctcgccatga agaggaggcg taagtgaagt tttttgccct
    20581 cttcatttac cgcccggtgg cgacgatttt actgtcggtt gccattaccc tgtgcggcat
    20641 actgggtttc cgtatgctgc cggtcgcccc gctgccgcag gtcgattttc cggtgattat
    20701 ggtcagcgcc tcgctgcccg gtgcatcgcc ggagacaatg gcgtcttccg ttgccacgcc
    20761 gctggagcgc tcacttgggc gcattgccgg ggtcagtgaa atgacctcca gtagttcgct
    20821 cggcagcacg cgcattattt tgcagtttga ttttgaccgg gatatcaacg gcgcagcgcg
    20881 tgatgtgcag gcggcgatca acgctgcaca aagtttgctg cccagtggga tgcccagccg
    20941 tccgacctat cgcaaagcga acccgtcgga tgcgccaatt atgatcctga cgctaacctc
    21001 cgatacttat tcgcagggtg aattgtacga tttcgcctcg acgcagctgg ctccgacgat
    21061 ttcgcaaatc gacggcgtcg gtgatgtcga tgtcggcggc agctcgctgc ccgccgtgcg
    21121 cgtcgggctg aacccgcagg cgctgtttaa tcagggcgtg tcgctggacg acgtacgcac
    21181 tgccatcagc aatgccaacg tgcgtaaacc gcagggggcg ctggaagatg gcactcaccg
    21241 ctggcagatc cagaccaacg atgaactgaa aaccgccgct gaatatcagc cgctgattat
    21301 tcactacaac aacggcggcg cggttcgtct gggcgatgtg gcgacggtga ccgactcagt
    21361 gcaggatgtg cgcaacgccg ggatgaccaa cgccaaaccg gctattttgc tgatgatccg
    21421 caagctgccg gaagccaata ttatccagac ggttgacagc atccgggcaa aattaccgga
    21481 gttgcaggaa accattccgg cggcgattga tctgcaaatt gctcaggatc gctcccccac
    21541 cattcgcgcc tcgctggaag aagtcgagca aacgctgatt atctcggtgg cgctggtgat
    21601 tctggtggtc tttttattcc tgcgctcggg tcgcgccact attattcccg ccgttgcggt
    21661 gccggtttcg ctgattggta cgtttgcggc gatgtacctg tgcggattca gtctcaataa
    21721 cctgtcgtta atggcgctca ccatcgctac tggtttcgtg gtggatgacg ccatcgtggt
    21781 gctggaaaac attgcccgtc atctggaagc ggggatgaaa ccactacagg ccgcgctaca
    21841 aggtactcgc gaagtcggct ttacggtgct gtcgatgagt ctgtcactgg tggcggtgtt
    21901 cctaccgctg ctgttgatgg gcggattgcc gggccgactg ttacgcgagt ttgccgtgac
    21961 gctttctgtc gccattggta tttcgttgct ggtttctctg acattaacgc caatgatgtg
    22021 tggctggatg ctgaaagcca gcaagccgcg cgagcaaaag cgactgcgtg gttttggtcg
    22081 catgttggta gccctgcaac aaggctatgg taagtcactg aaatgggtgc tcaatcatac
    22141 ccgtctggtg ggcgtggtgc tgcttggcac cattgctttg aatatctggc tatatatctc
    22201 gatcccgaaa accttcttcc cggagcagga cactggagtg ttgatgggcg ggatacaggc
    22261 ggatcagagt atttcgtttc aggcgatgcg cggtaagttg caggatttta tgaaaattat
    22321 ccgtgacgat ccggcagtgg ataatgtcac cggctttacc ggcggttcac gagttaacag
    22381 tgggatgatg tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat
    22441 tatcgaccgt ctgcgggtga agctggcgaa agaaccgggg gcgaatctgt tcctgatggc
    22501 ggtacaggat attcgcgttg gcggacgtca gtctaacgcc agttatcagt acacgttgtt
    22561 atccgacgac ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac
    22621 attgccggaa ctggcggacg tgaactccga ccagcaggat aacggcgcgg agatgaatct
    22681 ggtttacgac cgcgacacca tggcacggct gggaatcgac gtacaggccg ccaacagcct
    22741 gttaaataac gccttcggtc agcggcaaat ctcgaccatt taccagccga tgaaccagta
    22801 caaagtggtg atggaagtgg atccgcgcta tacccaggac atcagcgcgc tggaaaaaat
    22861 gttcgttatc aataacgaag gaaaagcgat cccgctgtcg tatttcgcta aatggcaacc
    22921 ggcgaatgcc ccactatcgg tgaatcatca gggattatcg gcagcctcga ccatctcgtt
    22981 caacctgccg accggaaaat cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac
    23041 ccagcttggt gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc aggtgttcca
    23101 ggagacgatg aactcacagg tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt
    23161 gctgggtatc ctttacgaga gttatgtaca tccgctgacg attctctcca ccctgccctc
    23221 ggcgggcgtt ggggcgctgc tggcgctgga gctgttcaat gccccgttca gcctaatcgc
    23281 cctgataggg atcatgctat taatcggcat tgtgaagaaa aacgccatta tgatggtcga
    23341 ttttgcgctt gaagcccaac ggcacggtaa cctgacgccg caggaagcta ttttccaggc
    23401 ctgtctgctg cgttttcgcc cgattatgat gactaccctg gcggcgctgt ttggcgcgtt
    23461 gccgctggtg ttgtcgggcg gcgacggctc ggagctgcgc caacccctgg ggatcaccat
    23521 tgtcggcggt ctggtaatga gccagctcct gacgctgtat accacgccgg tggtgtatct
    23581 ctttttcgac cgtctacggc tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta
    23641 aatgacagat cttcccgaca gcacccgctg gcaattgtgg attgtggctt tcggcttctt
    23701 tatgcagtcg ctggacacca ccatcgttaa caccgcgctt ccctcaatgg cgcaaagcct
    23761 gggggaaagt ccgttgcata tgcacatggt tattgtctct tatgtgctga ccgtggcggt
    23821 gatgctgcct gccagcggct ggctggcgga caaagtcggc gtgcgcaata ttttctttac
    23881 cgctatcgtg ctgtttactc tcggttcact gttttgcgcg ctttccggca cgctgaacga
    23941 actgttgctg gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc cgatcggaag
    24001 gcttacggtg atgaaaatcg taccgcgcga gcaatatatg gcggcgatga cctttgtcac
    24061 gttacccggt caggtcggtc cgctgctcgg tccggcgctt ggcggcctgc tggtggagta
    24121 cgcgtcgtgg cactggatct ttttgatcaa cattccggtg ggaattattg gtgcgatcgc
    24181 cacattgatg ttaatgccga actataccat gcagacgcgg cgctttgatc tctccggctt
    24241 tctgttgctg gcggttggca tggcggtatt aactctggcg ctggacggca gtaaaggtac
    24301 aggtttatcg ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct
    24361 ttatctgctg cacgccagaa ataacaaccg cgctcttttc agtctgaaac tgttccgcac
    24421 tcgtaccttt tcgctgggcc tggcggggag ctttgccgga cgtatcggta gcggcatgtt
    24481 gccctttatg acgccggttt tcctgcaaat tggccttggc ttctcaccgt ttcatgccgg
    24541 actgatgatg atcccgatgg tgcttggcag catgggaatg aagcgaattg tggtacaggt
    24601 ggtgaatcgc tttggttatc gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt
    24661 caccctgttg tttatgacta ccgctctgtt gggctggtac tacgttttgc cgttcgtcct
    24721 gtttttacaa gggatggtca actcgacccg tttctcctcc atgaacaccc tgacgctgaa
    24781 agatctcccg gacaatctgg cgagcagcgg caacagtctg ctgtcgatga ttatgcaatt
    24841 gtcgatgagt atcggcgtca ccatcgccgg gctgttgctg ggactttttg gttcgcagca
    24901 tgtcagcgtc gacagcggca ccacacaaac cgtctttatg tacacctggc ttagcatggc
    24961 gtttatcatc gcccttccgg cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa
    25021 tgtagctatt tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc cggtattacc
    25081 ggcaaactgt ttctggcgat tttcgccacc tgcattgtct tgctgatcag tatgcactgg
    25141 gcggtgcgta ttagttttga gcgtggcttt attgattaca tcaagcatgg taatgaacag
    25201 cgattacaac tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg caactggcgc
    25261 ttcctgcgca acaatgatcg ctttgtcttt cagatcctgc gttcatttga acacgataat
    25321 tcggaagata aacccggccc gggtatgcca ccgcacggct ggcgtaccca gttctgggtg
    25381 gttgatcaaa acaacaaagt gctggttggt ccgcgagcgc cgattccacc tgacggtaca
    25441 cggcgaccca ttctggtcaa cggtgcggaa gttggcgcgg tgatcgcctc ccccgttgag
    25501 cggttaacgc gcaatactga tatcaatttc gataaacaac agcggcaaac cagctggctg
    25561 attgtcgccc tggcaacgtt actcgcggcg ctcgccacct ttctgctggc gcgtggtctt
    25621 ttggccccgg tcaaacggct ggtggacggc acgcacaaac tggcggcggg cgatttcact
    25681 acccgcgtaa cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag
    25741 cttgccagca cgctggagaa aaaccagcaa atgcggcgcg attttatggc cgatatttct
    25801 cacgaactgc gtacgccatt agcggtgctg cgcggtgaac tagaagctat tcaggatggc
    25861 gtgcgcaaat tcacgccgga gacggtggcg tctttacagg cggaggtcgg tacactgacc
    25921 aaactggttg acgatcttca tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa
    25981 aaagcaccgg tagatttgat cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga
    26041 ttcgccagcc gtggcctgaa actgcaattt tccctgccag acagtattac cgtatttggc
    26101 gatcgcgacc gtttaatgca gttgttcaat aacttactgg aaaacagcct gcgctacact
    26161 gacagcggcg gcagcctgaa aatctctgcc gagcagcacg acaaaacggt gcgcctgacc
    26221 tttgccgaca gtgcgccagg tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt
    26281 tatcgcaccg aaggttcccg caaccgtgcc agcggcggtt ccgggctggg gctggcgatt
    26341 tgcctgaaca ttgttgaagc acataatggc cgcattattg ccgcccattc gccttttggc
    26401 ggggtaagca ttacagtaga gttaccgctg gaacgggatt tacagagaga agtatgaccg
    26461 agttaccaat cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg
    26521 ggcagttgct cattgattat ctgcgtgctg cgagctatgc gccgacgctt atcagtcacg
    26581 gcgatcaggt actgccgtat gtgcgccaga caccaccgga tctgatcctg ttggatctga
    26641 tgctccctgg caccgatggc ctgacgctgt gccgggaaat tcgtcgtttt tctgacattc
    26701 ctatcgtgat ggtgacagca aaaatcgaag agatcgaccg cctgctgggg ctggagattg
    26761 gcgcagatga ctacatctgc aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa
    26821 ccattttgcg ccgttgcaaa ccgcagcgtg agttgcaaca acaggatgct gaaagcccgt
    26881 tgattatcga cgaaggccgt tttcaggctt catggcgcgg taaaatgctt gacctgacgc
    26941 ctgcggaatt tcgtctgctg aaaacgctct ctcatgaacc aggaaaagtg ttctcccgcg
    27001 agcaattgct caatcatctt tatgacgact accgcgtagt aaccgaccgc accatcgaca
    27061 gccacattaa aaacctgcgc cgcaagctgg aatctctcga cgccgaacag tcatttatcc
    27121 gcgccgttta cggcgttggt taccgctggg aagccgacgc ctgccgcatc gtttagtttt
    27181 agcgacatga ctttgttagc cggagatggc gttcagaccc gatccggcaa cattatccca
    27241 cgcatggtca gcaaactgac attatctctc cgccctggca ggcatcagaa aatcgctcat
    27301 actttaatcg gtaaacagca cctttagatg ctgttttcga tacacaattt caatcaagga
    27361 gtcattatgg ctggttggtt tgaactcagt aagagcagtg ataatcagtt ccggtttgtg
    27421 ctaaaagcgg gcaatggtga gactatcctc accagcgagc tgtatacttc aaaagcctct
    27481 gcggaaaagg gcatcgcgtc ggtgcgtagc aacagcccgc aagaagaacg ctatgagaaa
    27541 aaaacggcaa gcaacggcaa attctatttc aatctgaaag ccgccaatca tcaaattatc
    27601 ggctccagcc agatgtatgc caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa
    27661 gccaatggca caagccagac ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt
    27721 gttggcgtcc ggctttattt ccaaataacg cttatttatt tacctgcgcc attaaaatag
    27781 tggacactga tgaatactca ttgtgctttc tttgtttaac atctccatgc ttatcctcct
    27841 gaacatgttc atctgccaaa atatcttcta atacttttcg tgacgctatc cggtttatga
    27901 catacactga tgtaagattt ttacttacag gagagcataa acatggccag aacgatgacg
    27961 gttgatctcg gcgatgagtt gcgtgaattt atcgagtcct taattgaatc cggcgactat
    28021 cgcacccaaa gcgaagtgat ccgtgagtca ctgcgcttac ttcgagaaaa gcaagcagag
    28081 tctcgcctgc aagcattacg tgacatgctt gcagaagggt tgagtagtgg tgaagctcag
    28141 ccgtgggaaa aggacgcgtt tttacggaag gtgaaagcag ggataaggaa ataatgtgcg
    28201 tattattaaa ctcatgccaa aagccaatga ggatttagaa ggcatctggt attatagtta
    28261 tcaccatttc ggcgaaccac aagccgacag atatgtggaa catctttcag acgttctcca
    28321 gattctgagt aacaataata taggtacgcc cagacctgaa ttaggcgaag gtatttttgt
    28381 tcttcctttt gaacgccacg taatttattt cctgcaatca ccaggggaaa ttatcgtcat
    28441 tcgtatcctt aaccaaaacc aggacgccac cagacattta cactggagct aaacccgccg
    28501 attgcgctac aatgcccgcc cttaaagtgg gggcactccc ctaaccgctt catcaggtga
    28561 agcggatctg acctgtcatc agaacgagag aattatgttt aaaccggaac tcctttcccc
    28621 ggcgggaacg ctgaaaaata tgcgttacgc tttcgcttat ggcgcagatg ctgtttatgc
    28681 gggccagccg cgttactccc tgcgtgtgcg caacaacgaa ttcaaccacg aaaaccttca
    28741 gctcggcatc aatgaagccc acgcgctggg gaaaaagttt tatgtcgtgg tcaacattgc
    28801 accgcacaac gccaagctga aaacctttat ccgtgacctg aaaccagtgg tggaaatggg
    28861 gccggatgcg ctgattatgt ccgatccagg gctgattatg ctggtgcgtg agcacttccc
    28921 tgaaatgccg atccatctct cggtacaggc taacgccgtg aactgggcga cggtgaaatt
    28981 ctggcagcaa atgggcctga cccgcgtgat cctctctcgc gagctgtcgc tggaagagat
    29041 tgaagagatc cgcaatcagg tgccggatat ggagatcgag atcttcgttc acggcgcact
    29101 gtgcatggcc tactccggtc gctgcctgct ctctggctat atcaacaagc gcgatccgaa
    29161 ccagggtacc tgcaccaacg cctgccgctg ggagtacaac gtccaggaag ggaaagaaga
    29221 tgacgttggc aacatcgtac acaagtacga gccgattccg gtgcaaaatg ttgagccgac
    29281 gctgggtatc ggcgcgccaa ccgacaaagt gtttatgatc gaagaagccc agcgtccggg
    29341 cgagtatatg accgcgtttg aagatgagca cggcacttac atcatgaact cgaaagatct
    29401 gcgcgctatc gcccatgttg aacgcctgac caaaatgggc gtgcattcgc tgaaaatcga
    29461 aggccgtacc aaatctttct actattgcgc acgcaccgca caggtttacc gcaaagctat
    29521 tgatgacgcc gctgcgggca aaccgtttga caccagcctg ctggaaaccc tggaaggcct
    29581 ggcgcatcgc ggttacaccg aaggattcct gcgtcgtcat actcacgacg attatcagaa
    29641 ctacgaatac ggttattcgg tttctgaccg ccagcagttt gttggtgagt ttaccggtga
    29701 gcgcaagggg gacctcgcgg cggtagcggt gaaaaataaa ttctccgttg gcgacagcct
    29761 tgagctgatg acgccgcaag gcaacattaa ctttaccctt gagcacatgg aaaacgccaa
    29821 aggtgaagcg atgccggtcg caccaggcga tggttatact gtgtggctcc cggtgccgca
    29881 ggatcttgag ctcaattacg cgctgctgat gcgtaatttc tccggggaaa ccacgcgtaa
    29941 cccccacggt aagtgattaa tttcgattat ttttcccgga tggaaaattc ttagaaaccg
    30001 atcacataca gctgcattta ttaaggttat catccgtttc gctgaaaaac ataacccata
    30061 aaatgctagc tgtaccagga accacctcct tagcctgtgt aatctccctt acacgggctt
    30121 attttttatg cataagccct atccctggtc accgtcttcc attgaccaca tcgatagaat
    30181 ctcccatcat agcacgatgc ctttcacgta acggcatcgt gctcgcacag gttccggcta
    30241 cgcacagcca gaacgcgcat gtttgacgct taccaaaaaa tattctcact ctacacattt
    30301 gaatgtcaga cgagcgacgc catgtaatcc tgcaccttct gtcttcaggt caactatctg
    30361 catttttttg ccctgagtaa cacagaaatg ggctgcatca ttttttacta tattttctgc
    30421 accagatatt ctccccctgg ctaaagaagc ttcggcttcg gtgtagtatt ggttatcgag
    30481 tttacgctga atattacttt tatatgcaag accaaattta ccgatacttg tctcatcatt
    30541 atgcacagca caaccagaca taagaaaaat actaaataat gatatagcaa ctatcttttt
    30601 catctcacct ttccccatta aatacca
//
LOCUS       DAZKFU020000031        29688 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZKFU020000031 DAZKFU020000000
VERSION     DAZKFU020000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.031"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..1720
                     /gene="rtcR"
                     /locus_tag="ACHOQQ_RS05590"
                     /old_locus_tag="pgaptmp_001118"
     CDS             122..1720
                     /gene="rtcR"
                     /locus_tag="ACHOQQ_RS05590"
                     /old_locus_tag="pgaptmp_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGU4469395.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIVSVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLEVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEALQDQHSSS"
     gene            complement(1702..2460)
                     /locus_tag="ACHOQQ_RS05595"
                     /old_locus_tag="pgaptmp_001119"
     CDS             complement(1702..2460)
                     /locus_tag="ACHOQQ_RS05595"
                     /old_locus_tag="pgaptmp_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGU4469396.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2477..3307)
                     /gene="glpG"
                     /locus_tag="ACHOQQ_RS05600"
                     /old_locus_tag="pgaptmp_001120"
     CDS             complement(2477..3307)
                     /gene="glpG"
                     /locus_tag="ACHOQQ_RS05600"
                     /old_locus_tag="pgaptmp_001120"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGU4469397.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3352..3678)
                     /gene="glpE"
                     /locus_tag="ACHOQQ_RS05605"
                     /old_locus_tag="pgaptmp_001121"
     CDS             complement(3352..3678)
                     /gene="glpE"
                     /locus_tag="ACHOQQ_RS05605"
                     /old_locus_tag="pgaptmp_001121"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGU4469398.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3868..5373
                     /gene="glpD"
                     /locus_tag="ACHOQQ_RS05610"
                     /old_locus_tag="pgaptmp_001122"
     CDS             3868..5373
                     /gene="glpD"
                     /locus_tag="ACHOQQ_RS05610"
                     /old_locus_tag="pgaptmp_001122"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGU4469399.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLNVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHFARTYGSNSELLLGNAGAVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLSAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5588..6178)
                     /locus_tag="ACHOQQ_RS05615"
                     /old_locus_tag="pgaptmp_001123"
     CDS             complement(5588..6178)
                     /locus_tag="ACHOQQ_RS05615"
                     /old_locus_tag="pgaptmp_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469400.1"
                     /translation="MNKIRLYIYPLYLGFVIVPLLVWPTVVVVGITLSFLMEMLFSNS
                     FMHRRISLLELQLWLSAEYALFFCVMCCVGWQFTRRTQSELKSRLHCWLVFAPVYLWL
                     ILWNIIFYIAPAKIALLESIRNFFLLVVWLPLNFSPFWPQPWTDFIGPISAQLGFALG
                     YYCQWRSKNRCQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(6193..6957)
                     /locus_tag="ACHOQQ_RS05620"
                     /old_locus_tag="pgaptmp_001124"
     CDS             complement(6193..6957)
                     /locus_tag="ACHOQQ_RS05620"
                     /old_locus_tag="pgaptmp_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469401.1"
                     /translation="MQCSPAMQALIEQIYHIFRRYPVPSRFHVCCPYCFSEEQQQALR
                     GVSLRAIPFPLLHAWGTSIGPTPQNDDEIRYLLPRLFEFIAQRQFPGIYEGCCLQRIA
                     EADNQNWRTDELQIISDFAYLYMQDWVSAKEVVELEIILEMFYRGGIKIEPLLDAIMS
                     ISGYWPTASMACLLWRNSKEYVENSDFEQSDDNKTITTQINTWARNNHPLLKERARQA
                     IENPLKQPEPMTEYQVWEDDWMIDECLCAMYDASPE"
     gene            complement(7060..8568)
                     /locus_tag="ACHOQQ_RS05625"
                     /old_locus_tag="pgaptmp_001125"
     CDS             complement(7060..8568)
                     /locus_tag="ACHOQQ_RS05625"
                     /old_locus_tag="pgaptmp_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4469402.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWVGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDIWAW
                     QPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWDYLE
                     NSRTPEAYNKIVKGDADIIFVAQPSGGQKKRAKESGVTLLYTPFAREAFVFIVNADNP
                     VNSMTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIIDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(8693..11140)
                     /gene="glgP"
                     /locus_tag="ACHOQQ_RS05630"
                     /old_locus_tag="pgaptmp_001126"
     CDS             complement(8693..11140)
                     /gene="glgP"
                     /locus_tag="ACHOQQ_RS05630"
                     /old_locus_tag="pgaptmp_001126"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGU4469403.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11159..12592)
                     /gene="glgA"
                     /locus_tag="ACHOQQ_RS05635"
                     /old_locus_tag="pgaptmp_001127"
     CDS             complement(11159..12592)
                     /gene="glgA"
                     /locus_tag="ACHOQQ_RS05635"
                     /old_locus_tag="pgaptmp_001127"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGU4469404.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(12592..13887)
                     /gene="glgC"
                     /locus_tag="ACHOQQ_RS05640"
                     /old_locus_tag="pgaptmp_001128"
     CDS             complement(12592..13887)
                     /gene="glgC"
                     /locus_tag="ACHOQQ_RS05640"
                     /old_locus_tag="pgaptmp_001128"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGU4469405.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(13905..15878)
                     /gene="glgX"
                     /locus_tag="ACHOQQ_RS05645"
                     /old_locus_tag="pgaptmp_001129"
     CDS             complement(13905..15878)
                     /gene="glgX"
                     /locus_tag="ACHOQQ_RS05645"
                     /old_locus_tag="pgaptmp_001129"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGU4469406.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDTNGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(15875..18061)
                     /gene="glgB"
                     /locus_tag="ACHOQQ_RS05650"
                     /old_locus_tag="pgaptmp_001130"
     CDS             complement(15875..18061)
                     /gene="glgB"
                     /locus_tag="ACHOQQ_RS05650"
                     /old_locus_tag="pgaptmp_001130"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGU4469407.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18334..19437)
                     /gene="asd"
                     /locus_tag="ACHOQQ_RS05655"
                     /old_locus_tag="pgaptmp_001131"
     CDS             complement(18334..19437)
                     /gene="asd"
                     /locus_tag="ACHOQQ_RS05655"
                     /old_locus_tag="pgaptmp_001131"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4469408.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            19629..20222
                     /gene="yhgN"
                     /locus_tag="ACHOQQ_RS05660"
                     /old_locus_tag="pgaptmp_001132"
     CDS             19629..20222
                     /gene="yhgN"
                     /locus_tag="ACHOQQ_RS05660"
                     /old_locus_tag="pgaptmp_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGU4469409.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20268..21563)
                     /locus_tag="ACHOQQ_RS05665"
                     /old_locus_tag="pgaptmp_001133"
     CDS             complement(20268..21563)
                     /locus_tag="ACHOQQ_RS05665"
                     /old_locus_tag="pgaptmp_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGU4469410.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(21470..23566)
                     /locus_tag="ACHOQQ_RS05670"
                     /old_locus_tag="pgaptmp_001134"
     CDS             complement(21470..23566)
                     /locus_tag="ACHOQQ_RS05670"
                     /old_locus_tag="pgaptmp_001134"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HGU4469411.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(23706..25046)
                     /gene="gntU"
                     /locus_tag="ACHOQQ_RS05675"
                     /old_locus_tag="pgaptmp_001135"
     CDS             complement(23706..25046)
                     /gene="gntU"
                     /locus_tag="ACHOQQ_RS05675"
                     /old_locus_tag="pgaptmp_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4469412.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(25050..25577)
                     /gene="gntK"
                     /locus_tag="ACHOQQ_RS05680"
                     /old_locus_tag="pgaptmp_001136"
     CDS             complement(25050..25577)
                     /gene="gntK"
                     /locus_tag="ACHOQQ_RS05680"
                     /old_locus_tag="pgaptmp_001136"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU4469413.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(25716..26711)
                     /gene="gntR"
                     /locus_tag="ACHOQQ_RS05685"
                     /old_locus_tag="pgaptmp_001137"
     CDS             complement(25716..26711)
                     /gene="gntR"
                     /locus_tag="ACHOQQ_RS05685"
                     /old_locus_tag="pgaptmp_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGU4469414.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTAAIIARGHRHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(26935..27630)
                     /gene="yhhW"
                     /locus_tag="ACHOQQ_RS05690"
                     /old_locus_tag="pgaptmp_001138"
     CDS             complement(26935..27630)
                     /gene="yhhW"
                     /locus_tag="ACHOQQ_RS05690"
                     /old_locus_tag="pgaptmp_001138"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGU4469415.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(27753..28790)
                     /locus_tag="ACHOQQ_RS05695"
                     /old_locus_tag="pgaptmp_001139"
     CDS             complement(27753..28790)
                     /locus_tag="ACHOQQ_RS05695"
                     /old_locus_tag="pgaptmp_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4469416.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            29125..29613
                     /gene="yhhY"
                     /locus_tag="ACHOQQ_RS05700"
                     /old_locus_tag="pgaptmp_001140"
     CDS             29125..29613
                     /gene="yhhY"
                     /locus_tag="ACHOQQ_RS05700"
                     /old_locus_tag="pgaptmp_001140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4469417.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         7436 a         7955 c         7272 g         7025 t
ORIGIN      
        1 gcagattaat tttttaattc attgttttaa aaaagattat atctttacgt ccgtaaccgg
       61 agatttcccg caaagccgat ttaccgataa tgaaatatcg tcttttataa ggatatctaa
      121 gatgcgtaaa acagtggctt ttggctttgt cggcaccgta ctggattatg ccggacgcgg
      181 cagtcagcgc tggtcgaaat ggcgtccgtc actctgtata tgccagcaag aatcgttagt
      241 catcgatcga ctggaattat tgcacgacgc ccgctcgcgc tcgctgtttg aaacgcttaa
      301 acgcgatatc gtcagcgttt cgccagaaac agaagtggtg agcgttgaga ttgagctgca
      361 taacccgtgg gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacga
      421 gtttcagcca gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca
      481 gatttgctgg tttctgctgg cggaagcgcg ttacctgccc gcccggttga tacaatcttc
      541 acctccgcgc aaaaaagaac agccgcgcgg cgcaggtgaa gtgacgatta tcgatctcga
      601 tttaagccgt tataacgcca tcgccagccg ctttgccgag gaacgccagc aaacgcttga
      661 ttttcttaag tccggtattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat
      721 tgaaaaagtg gcgattaaat cccgcgcacc gatcctgcta aacggcccaa ccggtgcggg
      781 caagtcattt ctggcgcgac gtatatttga attaaaacag gcgcggcatc agtttagcgg
      841 cgcgtttgtg gaagtgaact gcgcaaccct gcgcggcgat accgccatgt cgacgctgtt
      901 tggtcatgtg aaaggcgcgt ttaccggggc gcgggaatct cgtgaaggat tattacgcag
      961 cgccaacggc ggaatgttgt ttcttgatga gattggcgaa ctgggcgcag acgaacaggc
     1021 aatgctgctt aaagccattg aagaaaaaac cttttatccg tttggcagcg atcgccaggt
     1081 gagcagtgat tttcagctta tcgccggaac ggtgcgtgat ttgcgccagc tggttgccga
     1141 aggaaaattt cgcgaagacc tgtacgcgcg gatcaatctc tggaccttca ccctgccggg
     1201 gctacgccag cgtcaggaag atattgaacc taacctggat tatgaagtgg agcgccacgc
     1261 ttcactcacc ggcgacagcg tgcgttttaa caccgaagcg cggcgcgcct ggctggcctt
     1321 tgcgacatct ccccaggcaa catggcgcgg taactttcgc gagctttctg ccagcgtcac
     1381 gcggatggct acctttgcca ctagcggacg catcactctg gaagtggtgg aagatgagat
     1441 aaaccgtctg cgctataact ggcaggagag tcgcccctcc gcgcttacgc agctactggg
     1501 cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg ttatcgctat
     1561 ctgccgccag gcaaagtcac tttctgcagc cggacgacag ctttttgatg tttcgcgcca
     1621 gggcaaagcc agcgtcaacg acgcggatcg gctacgcaaa tacctggcgc gttttggtct
     1681 gacgtgggaa gcattgcagg atcagcacag ctccagttga atatggtggt ccgtcagcac
     1741 ctgcatcacg cttactggcg gcggggcatc ggtgtagacg gcatctacca tgctgatgct
     1801 gcccatattg accatcgcgt tacggccaaa tttcgagtga tcgacaacca gcataacgtg
     1861 gcgcgagttc tcaataatgg cgcgtttggt gcgaacttcg tggtaatcga actccagcag
     1921 cgagccgtcg ctatcgatgc cgcttatccc cagaatgccg aaatcaaggc ggaactggga
     1981 gataaaatcg agcgtcgctt cgccaatgat cccgccatcg cggctgcgta attcgccacc
     2041 ggcgagaata atacggaaat cctcttttac catcagcgtg ttagcaacgt taagattgtt
     2101 ggtgacaatg cgcaaattgc tgtgattaag cagcgcgtgc gccaccgctt ccggcgtggt
     2161 gccgatatcg ataaacagcg tcgagccgtt ggggatttgc tccgccactt tacgggcgat
     2221 gcgctctttt tcttcggtct gggtggcttt acgatcgtgc cacggcgtgt taaccgaact
     2281 ggaaggcagt gccgcaccgc catgatggcg cagaatcagg ttttgctccg ccagctcatt
     2341 gagatcgcgg cgaatagtct gcgggctgac ggagaagtgc tccaccagct cctcggtgct
     2401 gacataaccc tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat
     2461 ttataaatcc ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg
     2521 ctaaacccac ggctaacccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat
     2581 caaaccatcc ggcgacaatc cagatcagcg caaagataat taacccacgt tgcaggtaaa
     2641 tgccactttg cggatcgcgt tcgccacgta gccagacgta gcccatcagc gcatacacca
     2701 cgccagaaag cccgccaaac cacggcccgc tgaatttttg ctgcacatag ccgcttaaca
     2761 gggcgctaat gagagtaata acaattagct taccgctacc gaggcgtttt tccaccgcgc
     2821 cgccgagata ccaccaccag agcaggttaa agaggatatg catcagcgag aagtgcatta
     2881 acgcgtgggt gaagtaacgc cagaactcaa atttcagtgc tggatcgaat ggccaggcca
     2941 gccataacat cacttcctga tcgccgagaa tttgcatggc aataaacacc accacgcagg
     3001 cgatcatcat cacccaggtt accggacctg cgcgttcacg caaggcggca aagaaaggat
     3061 aacggcgata atgcaggcca ctgccggtat ggcctgcctg ccagctcgcc gccagataac
     3121 gcggatctgc cgggttttcg agaaaacgcg ccagctccgc ccgtacgcgc tcggcctggg
     3181 actcatccgc cagccagaca tcgctttggt tatgttgttg aatcgtgagg ataacaccct
     3241 gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca
     3301 tcaacatcgt tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc
     3361 gtacgccacc tctgcgggaa actgacgttg ccaggcttca aagccgccgt caatgctata
     3421 gaccacatcg tagccctgtt gcagcagata ctgcgccgcg cctttgctgc tattgccgtg
     3481 ataacacatc accatcaccg gagtgtcaaa gtcgttatta cgcataaaag cgcccagcgt
     3541 gtcgttggtt aaatggaaag cctgcaccgc atgtcccatt gcgaaactct gtggatcgcg
     3601 aatatcgacc agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat
     3661 acattcgaac tgatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca
     3721 cagtttacgt cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt
     3781 ttttcaatgt tacctaaagc gctattcttt gctaatatgt tcgataacga acatttatga
     3841 gctttaacga aagtgaatga gggcagcatg gaaaccaaag atctgattgt gatagggggc
     3901 ggcatcaatg gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg
     3961 ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt
     4021 ggcctgcgct accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt
     4081 gaagtgctgc tgaaaatggc cccgcatatc gccttcccga tgcgttttcg cctgccacat
     4141 cgtccgcatc tacgcccggc gtggatgatt cgcattggtc tgtttatgta cgatcatctg
     4201 ggtaaacgca ccagcctgcc gggatcaact ggtttgcgtt ttggcgcaaa ttcagtgtta
     4261 aaaccggaaa ttaagcgcgg attcgaatat tccgactgtt gggtagacga tgcccgtctg
     4321 gtgctcgcca acgcccagat ggtagtgcgt aaaggcggcg aagtgcttac tcgtactcgt
     4381 gctacttctg ctcgtcgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc
     4441 ggcaaaaaat atagctggca agcgcgcggc ctggttaacg ccaccggccc gtgggtgaaa
     4501 cagttcttcg acgacgggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc
     4561 agccatattg tggtgccgcg cgtgcatacc caaaagcagg cctacattct gcaaaacgaa
     4621 gataaacgta ttgtgttcgt gatcccgtgg atggacgagt tttccatcat cggcaccacc
     4681 gatgtggaat acaaaggcga tccgaaagcg gtgaagattg aagagagtga aatcaattac
     4741 ctgctgaatg tgtataacgc gcactttaaa aagcagttaa gccgtgacga tatcgtctgg
     4801 acctactccg gtgtgcgacc actatgtgat gatgagtccg actcgccgca ggctattacc
     4861 cgtgattaca cccttgatat tcatgatgaa aatggcaaag caccgctgct gtcggtattc
     4921 ggcggtaagc tgaccaccta ccgaaaactg gcggaacatg cgctggaaaa actaacgccg
     4981 tattatcagg gtattggccc ggcatggacg aaagagagcg tgctaccggg tggcgccatc
     5041 gagggcgacc gcgacgatta tgccgctcgc ttacgccgcc gttatccgtt tctgaccgaa
     5101 tcgctggcac gtcatttcgc gcgtacttat ggcagcaaca gcgagctgct gctcggcaat
     5161 gcgggagcgg taagcgatct tggggaagat ttcggtcatg agttctacga agcggagctg
     5221 aaatatctgg tcgaccacga gtgggtccgc cgcgccgacg atgccctgtg gcgtcgcaca
     5281 aaacaaggta tgtggctaag tgcggaacaa caatctcgtg tgagtcagtg gttggtggag
     5341 tatacgcagc agaagctgtc gctggcgtcg taaattaacg taaggtgatc aggtcagatt
     5401 tcaatctggc ctgagactga tgacaaacac aaaattgcct gatgcgctac gcttatcagg
     5461 cctacgtggt ttatgcaata tattgaattt gcatgatttt gtaggcctga gaaggcattc
     5521 acgccgcatc aggcatgaac aaagtgccac gttgtcaaca atctgaccta ctttttgatt
     5581 cagggaatta ccgcaaagcc cacgccatca tacccaacgt aacaaaactt aagcacgttc
     5641 cccactcact ccacttccgt atctggcatc tatttttgct gcgccactgg caataatatc
     5701 ccaacgcaaa accaagctgt gcgcttattg gaccgataaa atccgtccac ggctgaggcc
     5761 aaaatggcga aaaattcagg ggtagccaga caacaagcag aaagaaattg cggatacttt
     5821 ccagcaacgc aattttcgct ggcgcgatgt aaaaaatgat attccagagg atcaaccata
     5881 aatagaccgg tgcgaatacc aaccaacaat ggagcctgct tttcaattca gattgagttc
     5941 tacgagtaaa ttgccaacca acgcaacaca ttacacagaa gaaaagcgca tattcagccg
     6001 ataaccaaag ttgtaattcc agaagggaaa tacgcctgtg catgaacgaa tttgagaaca
     6061 gcatctccat tagaaaactc agtgtgatac caacaacaac caccgtcggc cacaccaaca
     6121 acggcacaat aacaaagccg agatatagcg gataaatata aagtctgatt ttattcatct
     6181 ttattgtcct gattattcgg gtgatgcgtc atacatcgcg cataaacact catcaatcat
     6241 ccagtcgtct tcccagactt gatattctgt cattggttca ggctgtttta gtgggttctc
     6301 aatcgcttgt cgcgctcttt ctttcagcaa aggatgatta tttctagccc aggtattaat
     6361 ttgagtggta atagttttat tatcatcact ctgctcaaaa tcgctatttt cgacgtattc
     6421 tttactgtta cgccagagca aacatgccat agacgccgtc ggccagtaac cggaaatgga
     6481 cataatggcg tcaagtaagg gttcaatctt tataccaccg cgataaaaca tttctaatat
     6541 tatttccagt tccacgacct ccttagccga cacccaatcc tgcatatata aataggcgaa
     6601 atcgctaatt atttgtagtt catccgttcg ccagttctgg ttatctgcct ctgctattct
     6661 ctgtaaacag cagccttcat aaatgcctgg aaattgcctt tgggcaataa attcaaacaa
     6721 tctgggtaat aaatagcgaa tctcatcatc attttgcggg gtaggaccaa tacttgttcc
     6781 ccaggcatgt aataaaggga aaggtatggc acgcagtgaa acccctctta aggcttgttg
     6841 ttgttcttcg ctaaagcagt aaggacaaca aacatgaaat ctcgacggca ccggataacg
     6901 acgaaaaata tgataaatct gctcaattaa tgcctgcatg gcggggctgc attgcataat
     6961 tactccctct tccgctgatt gcgtgtaaac ggttgataaa caaaacgagc attggccgcg
     7021 ttcaggcgaa taataccggt atccatgtga ttatcctgct taaggtaagg ttttatacat
     7081 cggcacataa cctacatctt ccaccaaact ctgcccctgc ggcgttaaaa accattccac
     7141 caatttttgt gtctctgacg tcgtattttc cctcgttacc ataaaggcat cgatgatata
     7201 gggatatttg ccattgcgaa tattttccgc agtcggtgca ataccgttaa tcgctagcaa
     7261 ttttatattt ttatcagcat tcatttgcgt cgcgtaatag cggaaggtat agcctattgc
     7321 gttgtttgta ttacggtatt cggcaacgac tttaatcatt ccctccatca cgcttgccac
     7381 ttccgtttcc tgcggcgaga tcatgcggac atttttcatg acctgtgatt gcatcactgt
     7441 ctggctgcca gagtcttccg ggcgctgcca ggtctggatc tcctgatcgt taccgccaac
     7501 agtacgccag ttggtaattg caccactgaa aatgtcacgc acctgttgtt cagtcatgga
     7561 attaaccgga ttatccgcat tgacgatgaa aacgaatgct tcacgggcaa agggggtgta
     7621 cagcaaagtg acaccagatt cctttgcacg ttttttttgc ccaccggaag gttgcgccac
     7681 gaaaataata tcagcatcgc ccttaacgat tttgttatat gcttctggcg tacgagagtt
     7741 ttccagataa tcccaaacgt gaaaatcctc tggtattaca cttaatgcat aaaatgcaga
     7801 agcataaatt gggtacgcag ccgtggcgcc atcgattcgc ggccagtttt gcctaaactg
     7861 aatttgcggt ttgccacgca gcggcgtcag tcgattattg agtttatcgg gttgccaggc
     7921 ccagatatca atattttcgc gtacaccgac accaggattg agctgtttat ataaataacc
     7981 ctgataacca gcgacaaaac caagcatgag aaggataaac aggagggaat tacggcaacg
     8041 tagcccggat gtaccggtta aaggatgtct ggttggccgg ccgtacccca gcgaaaaact
     8101 gatatgcgcg ccaacgggta taatgactat ccaccagtac tcacctccga ctactgagac
     8161 taacaaaacg cccagccagg ggagcataag atcagccaac caacccgatc ccatctggtg
     8221 aaattgataa tctgccagat gcagcgcgat tatccagcca accagggtta atagcgcggg
     8281 taagagaaaa aagcattgcc gccagacggc tatttctctg ttttctggcg cgctatcacc
     8341 gacccacacg cgaccagaga aaaatattat tatcgccccc agcgtcagca taaagacgtt
     8401 gagtagaaga taaattgttg gcgtgataag gatattacaa acttcaataa taccggtaag
     8461 cccgacaccc agcatggcaa caaccgctgc caaaaattgc gccagtatgg ggatgccgaa
     8521 aaaagtcatt atcagcgagg tcaaaatcca ttttctattt ggcatcattc tgtccattct
     8581 tcctgaatga tggaattata ctccccgaga accctcgccc cctctggaca cttttccgaa
     8641 atgatggcga aaaaaacggg accctttggc cccgttctat ttattggtga aattacaatc
     8701 tcaccggatc gatatgccag atatgatcag cgtactcttt gatagtacgg tcagaagaga
     8761 agtagcccat attggcaatg ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt
     8821 aaagttcgtc aactttatcc tgacaatcga catagctgcg ataatccgcc agtacctggt
     8881 agtgatcgcc gaagttgatc agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg
     8941 gactgaatac accgctgccg atttgcgtca gcacctgatg cagctcctca tctttctcat
     9001 agtattcacg cggtttgtag ccctgacgac gcagttcttc cacttcttcc gctgtgttac
     9061 caaaaataaa gatattgtca gcaccgacat gatccagcat ctcgacgttt gcaccgtcca
     9121 gcgtaccgat agtcagcgcg ccgttaagcg caaacttcat gttactggtg ccggaagctt
     9181 ccgtccccgc cagcgaaatc tgttcagaca gatcggccgc cggaatgatc aactgcgcca
     9241 ggctaacgct gtagttcggg atgaacacga ctttcagctt gtcgccaatc tgcggatcgt
     9301 tgttgatcac tttcgctacg tcattgatca aatggataat atgcttcgcc atgtaatagg
     9361 ccgaagccgc cttaccgcca aaaatattca cgcgcggtac ccacttcgca tccgggtcgg
     9421 ccttgatgcg gttatagcgg gtaatcacat gcaacacatt catcaattga cgtttgtatt
     9481 cgtgaatacg tttgatttgt acatcgaaca acgccttagg attcaccacc acattcagct
     9541 gctgggcgat atactccgcc agacgctttt tgttctccag cttcgcctga tgcacagcgt
     9601 gattaaccat tgggaaatca cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc
     9661 gccagttacg gcccaggtgt tcgtccagca cggctgaaag cgatgggttc gctaccgcca
     9721 gccagcgacg cggcgtcaca ccgttggtga cgttggtgaa acgacccggg aagattttcg
     9781 caaagtcggc aaacaacgat tgcaccatca gattagagtg cagttccgat acaccgttaa
     9841 ctttgtggct cacaacaacc gccagccagg ccatacgcac acgacgaccg ttggattcat
     9901 caatgatcga cgcccgtccc agcagatcgg tatcgttcgg atactgttcc tgcaaggttt
     9961 tcaggaaata gtcgttgatt tcaaagatga tctgcaggtg acgcggcaga attttaccca
    10021 gcatatcaac cggccaggtt tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga
    10081 agacctgaca acacacctca aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca
    10141 gacgcatcat ctcaggaatc gacagtaccg gatgggtatc attgagatga atcgcgattt
    10201 tatccgccag gttatcgtag gttttatgca actgataatg gcggcttaaa atgtcctgaa
    10261 tggtcgagga aaccaggaag tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg
    10321 agtcatccgg atacagtacg cgagatacgt tctcggagtg gtttttatct tccactgccg
    10381 cgaagtagtc accctggttg aatttaccga ggttaatttc gctactggct tgcgcactcc
    10441 acaaacgcag cgtgttggtc gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga
    10501 ctcccagaat ctcttcggtt tcaatccagc gcgttttttt accttcctgc tgaatgcgac
    10561 caccaaaacg gactttatag cgcgtgttgt ggcgtttgaa ttcccacggg ttaccatatt
    10621 ccagccagta gtcaggcgac tctttctggc taccgttaac gatgttctgc ttgaacatac
    10681 cgtagtcata gcggatgccg taaccgcgcc ccggcaaccc taacgtcgcc agagaatcga
    10741 ggaagcaagc cgccagacgt cccaggccac cgttaccgag gcccgggtca ttttcttcat
    10801 caatcagctc ttcgagattt aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa
    10861 ttcctagcga caacatggcg ttggagagcg tacggccaat caaaaactcc atcgacaggt
    10921 agtaaacctg acgagtttct tgcgacaact gggcacggtt tgaacgtaac cagcgctcca
    10981 cgagacgatc gcgcacagca aataacgttg cgttcagcca ttcatgttta ttggcgacga
    11041 ccgggtcctt tccaatcgta aacatcagct tgtaagcgat agagtgctta agagcttcta
    11101 cgctaagcgt gggcgatgaa tatgtaaacg gagcattcat ataggcgttt cctgaaaact
    11161 atttcaagcg atagtaaagc tcacggtacg acttcgccgc gacctgccag ctaaaatcca
    11221 ttgccatagc ctgacgttgc acaaaccgcc acagtgaagg acgggaccac agtacaaaag
    11281 cacgtcgaat agcccgtaac agcgaccagg cattactatc ttcaaagaca aacccactgg
    11341 cgacgccatc tgcaaggttc tcaagagaac agtcagaaac cgtatcagca agcccaccgg
    11401 tgcgccgcac taacggcagc gtaccgtact tcaatccata aagttgcgtt aagccgcacg
    11461 gttcaaaacg gctgggcacc agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg
    11521 cttcgtgata gccaatctga acgcccactt ggccggggta ttccgctgcc gccgcaagga
    11581 aaccttcctg cagcaccgga tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca
    11641 gaaggcccgg taaggcttcc agcaccaggt cgagaccttt ctggctggtc agacggctca
    11701 ccactgcaaa aagcggcact ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc
    11761 gcttattttc cgctttatct tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt
    11821 ccgtctctgg actccagatt ttctcgtcca cgccgttcag tacgccggaa agacgccctt
    11881 cacggtgacg ctgttgcaac agaccttcca taccgtaggc aaactgcggt tcggtgatct
    11941 cgcgagcgta ggttggactg accgccgtaa tgtgatcggc atagtacaga ccggccttca
    12001 ggaaagagat ttgtccgttg aattccagcc catgaatatt aaagaatgac catggcaatt
    12061 ggatgtcatt catgtgatgt gcataaaaca tgccttgata ggccaggttg tgcacagtaa
    12121 acaccgactt cgccggacgc ccgcgcgccg ccagatacgc aggcgcaagg cctgcatgcc
    12181 agtcgtgcgc atgcaccaca tcaggacgcc agaatgggtc aagcccgctg gccatttctg
    12241 ccccaaccca ccccagcagc gcaaaacgca atacgttgtc ggtataggca aataagttgg
    12301 tatcgtgata cgggcttccc ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc
    12361 caaccccgtt gtaatgaccg aacaacagcg tgatatgccc ggcaaaggta tcacgacggg
    12421 atactacctg cgcatcggtc acgccacggc gaatatcggg aaatgcaggc aacagtacgc
    12481 gagcgtcaac gccgtctgcg atttgtgctg cgggtaatgc cccaataaca tcagccagac
    12541 cgccggtttt aagcagcggg aacatctctg aacatacatg taaaacctgc attatcgctc
    12601 ctgtttatgc cctaacttcc gtagcatttc gcgcgttacc agcacgatgc cttcttctga
    12661 acgatagaaa cgacgtgcat cttcctctgc gttttcacca atcaccatgc cttccggaat
    12721 aacacaagca cgatcgatga cgcagcggcg cagacggcac gagcgaccta cccatacttc
    12781 cggtaacaat acggcggaat caatgttgca gaatgaattc acgcgaacgc gcgagaacag
    12841 aacggactgc accaccaccg aaccggagat cacacaaccg ccggaaacca gtgagttaag
    12901 ggtcatcccg tggctaccgg agcgatcctg cacgaatttc gctggcggta atgattcatt
    12961 gtaggtgcga attggccaat tgcgatcgta catatccagc tccggcacca cagaggccag
    13021 atcgaggttc gctttccagt aagcttccag cgtacccaca tcgcgccagt acggctcggc
    13081 atccggatcg gattgtacgc aagagagcgg gaacgggtgc gcataggcca gaccggcttc
    13141 ggtgatcttg ggaatcaaat ctttgccaaa gtcgtggctg gagttctcat cgcgatcgtc
    13201 ttcttccagc agttcataca gatagtcggc gtcaaagacg tagataccca tactcgccag
    13261 agatttgctc ggatcgttcg gcattgacgg tgggttagca ggtttttcaa cgaattcgat
    13321 aattttatcg ttctcatcaa ccgccataac gccaaatgcg gaggcttctt caatcggtac
    13381 tggcatacaa gcaacggtgc aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga
    13441 gtagtcttgc ttgtagatat ggtcgcccgc caggatcacc acgtattccg ctttataacg
    13501 gcgaataata tcgaggtttt gggtgaccgc atctgcggtg ccgcgatacc agttttcccc
    13561 tttcattctc tgctgtgctg gcagcagatc gacaaactcg ttcatttctt cattgaagaa
    13621 tgaccagccg cgctgaatgt gctgcaccag agtgtgggac tggtactggg tgatcacgcc
    13681 catacgacgg atcccggagt tgatgcagtt agacagcgca aagtcgataa tgcggaactt
    13741 accgccgaag tgtacggccg gttttgctcg cttattggtt aaatccttca ggcgggtacc
    13801 acgtcctccc gccagtatca gggcaacaga tttcaatggc agctggcgcg ccaacattaa
    13861 gtgatcgttc ttctctaaac taaccatgac taactccttt tttatcatct ctggaacaca
    13921 cacaatccgt gtgcaggtcc ctgccagaca gccgtaatca ctgggttatc ctctccagcg
    13981 aatgggggaa tggcgtgcca ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg
    14041 gcgttaattg cgatcaaaaa gcgatccgag agcagaattt gcagctgttt cggcccgttt
    14101 tgccactcat ccgtgcttaa aggttgagca tatcgattta gccaacggac attgccgtcg
    14161 ccttcttccc accagcgatt ctccaccaaa gcgggaatac gcttgcgcag atggattaac
    14221 gcggcggtaa atgcggttaa accactgctt gcctgcgacc agtccaacca ggttaattgg
    14281 ttatcctgac agtaggcatt gttattgcca tgctggctgt gaccatgttc gtcaccggcc
    14341 agtaacatcg gcgtgccctg ggagagcaac aacgttgtta acagggcatg aatgctgtcg
    14401 cgccgccgtt caaccagatc aagagtaccg cctaaccctt ctttaccatg attgttactg
    14461 taattgttgt tggtcccgtc gcgattttct tctccgtttg cttcattgtg tttatggttg
    14521 aagcaaacgc agtcgcgaag cgtaaaaccg tcatgtgcgg tgacgagatt aatcgcggca
    14581 ctcggcagac gaccattacg tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac
    14641 gcccccagag gcaaatcata atgcagccag aaacgacggg cagcatcgcg gaaatgatcg
    14701 ttccactcgg caaacagcgg cgggaaattt cccacctgat aaccaccagg agcgatatcc
    14761 cacggttcag caattaactt cacctgcgag agcaccgggc agttctggat agcggtaaac
    14821 aacggcgcat cctgacggaa ctctggcgta cggcccatga ctgccgccag atcaaagcgg
    14881 aaaccatcga cgtggcaggt ttctacccaa taacgcaggc aggcgctggc ataatccacc
    14941 accgccgggt gactcaaatt gagcgtgttg ccacaaccgg tccagttgtg ataatcgccg
    15001 tcttctctta tccaataata gctacggttg tcgatcccac gcagcgagaa taacgggccg
    15061 tcgaggtcca gttccgcact atggttgagc acgatatcaa gaatgacttc gatacccgct
    15121 ttatgcagtg ctttgattgc atcgcgaaac tcatgcagcg ccgtttctgg cgagcaggca
    15181 tacgccggat gcagcgcaaa catcgccacc gggttgtaac cccagtagtt acttagcccc
    15241 atgcgttgca gacgtggttc actggcaaac tgcgccactg gcagcagttc cagcgcggta
    15301 atgcccaact gtttcaaata gttgatcatc accggatgcc cgagggcttt ataagtgcca
    15361 cggatctcga ccgggatctc cgggtgcagg tacgttaatc ctttaacatg ggcttcataa
    15421 atgatggtgc tgccccacgg cgtgcgcggc ggggcatcat cttcccagtc atagtgatca
    15481 accaccacta cgcatttcgg cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga
    15541 ccggcgtgca gcagcgggtt atctttaaac tccccgtcaa tttgccgcgc gcaaggatca
    15601 atcaacaact tcgccgggtt aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg
    15661 cgataaccat aacgcaaacc cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca
    15721 ctgtgccctg gcaagtcata gcgatgttcc tggccattgg tgtcaaagac acacagctct
    15781 acccgctcgg catgagcgga gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg
    15841 ccgaggggag cgggtttgcc aatggcgagt tgtgtcattc tgcctcccga accagccaga
    15901 tagtggccag cggtggtagc gtcaggctta gtgaatgctg acgaccatgg ctggcaatct
    15961 catcgctgtg taccgtgccg ccattgcctg cattactgcc gtgatagtgc atggaatcgg
    16021 tattgaggat ctcacgccat ttgcctggct gattaatgcc gaagcgataa tcatgacgcg
    16081 gcaccggcgt aaagttactg gcaacgatga tttcgttacc ctctttatcg cgacgcacaa
    16141 agatcagcac cgagcgttct ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa
    16201 aatccagttc atgcatcgct ttatggtggc ggtaggtgag gttcagatcg cgcaccagac
    16261 gctggacacc gtggtgccag ttatcgccgc cttccaacag atgccagtcg aggctggcgt
    16321 catggttcca ctcgcggccc tgggcaaatt cgttccccat gaacagcagt ttcttgcccg
    16381 gaaatgccca catccagcca tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc
    16441 ccggcatgcg gtcgagaatc gattttttac cgtggaccac ttcatcatgc gacaacggca
    16501 ggacgaagtt ttcagtgtag ttgtagagca tcccgaaggt cagtttatcg tgatgatact
    16561 gacgataaat cgggtcgagc ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc
    16621 acttgtacca gaagcccaga ccgcccatat cctgcggacg agaaacgcca gggaaatcgg
    16681 tagactcctc agccattgtc accgcaccgg aaacctgctc accaagaata cggttggtat
    16741 tacgcaagaa ttcaatcgct tcaagattct cgcgcccgcc aaattcgttc gggatccact
    16801 ccccctcttt acggctgtag tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg
    16861 catcaatacc aaaacgttca atccagtaaa gcgcgttacc gacgaggaag ttactgactt
    16921 cacggcgacc atagttgtaa atcagcgtgt tccagtcctg atgatagcct tcgcgcggat
    16981 cgctgtgttc atacaagttc gtaccatcaa attcggcaag cgcaaagtca tcagtcggga
    17041 agtggcctgg cacccagtcg agaatcacgt tcagaccagc tgcgtgtgca gcatcaatga
    17101 aataacggaa gtcgtcgcga gtaccaaaac ggcgggttgg tgcatacagg ccggttggct
    17161 gataacccca actgccatcg aagggatgct cgttaatggg cagtagttcg aggtgggtaa
    17221 agcccatcca tttagcataa ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga
    17281 aattgttgtc ggtgtggcga cgccaggagc ccaggtgaac ttcatatata gagattggcg
    17341 catcaaactg attcgctttt ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc
    17401 aaataagaga cgcggtttcc gggcgcattt gcgcttcgaa ggcataaggg tcggacttca
    17461 gacgcaagtt gccattggca tcaatcatct cgtatttata gagctgaccg ttatgcgccc
    17521 cagggataaa cagttcccag atgccgctct ctttacgcag gcgcatcggg tgacggcgac
    17581 cgtcccagta gttgaattgc ccaaccaccg agacccgacg ggcgtttgga gcccagacag
    17641 agaaacgcgt acctgtgacg ccatccatag tatctgcatg cgcgcctaaa gtttcatacg
    17701 ggcgcaggtg agtaccttca gataatagcc aggcatccat ttcctggatt agcggaccaa
    17761 aacggtaagg atcatcaatc aggttttgct gcccatgcca gacaacagcc aactgatagc
    17821 ggaaaaaatt cttacgtcgc ggaatgacgc cactaaagaa tccccgtgag tcgagacact
    17881 ccagttttgc gagtttgcgc ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg
    17941 gtaaaagggc acggacttcc agtcccgcgg tggttttatg cattcccagt acggaaaaag
    18001 gatccgcaaa atggcctgca attagcgcgt taatcacgtc tctatcgata cgatcggaca
    18061 tgcttgtctt cctgttttat tgtgtcaccc catccaattg gatttttgac ttctggttgt
    18121 gacatttttt tgacctgaac ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact
    18181 tcaggtaagg ctgtgaatac tcatgtattc cgccaccctt aaagaatagc caatgctcta
    18241 tttaactccc ggtaaatcat gaaacatctg cgcttactcc tgtattacgc actaacaggg
    18301 gcggcatcgc gccccagatt taatgaataa agattacgcc agttgacgaa gcatccgacg
    18361 cagcggctcc gcggcccccc acagcagctg gtcgcccacg gtaaaggctg acaggaactc
    18421 tggtcccata ttcagcttac gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc
    18481 agctggggtt agctcacgca tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg
    18541 attgtgcgca gccagcagtt cttccacggt cggaatcgac acatcttttt tcaatttaat
    18601 agtgaatgcc tggctgtggc agcgcaatgc cccgacacgc acacataaac catctaccgg
    18661 aattacggaa gatgtgttga ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg
    18721 gctctgaccg ttatcgagct gtttgtcgat ccacggaatc aggctacccg ccagcggcac
    18781 gccaaagtta tccaccggca gctcaccgct acgggttaag gttgtgactt tgcgttcgat
    18841 atcgagaata gcagaggacg gggtcgcgag ttcatctgct acatggccat acagatggcc
    18901 catctgggtt aataactcac gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt
    18961 tgcaacggac acccaatcaa caagatcatt ggcgaataaa ccacccaacg acatcaacat
    19021 caggcttacg gtacagttac cgccaacaaa agtcctgatg ccattattta atccgtcggt
    19081 aatgacgtcc tgattgacgg ggtcaagaat aatgatggcg tcatctttca tgcgcagaga
    19141 cgatgctgcg tcaatccagt aaccctgcca tccgctttca cgaagctttg gatagatttc
    19201 gttggtataa tcgccgccct gacaggtcac aatgatatcg agggccttta gcgcctccag
    19261 atcaaaggca tcctgaagtg tgccagtggt tccgccaaaa gacggcgcag cctgaccaag
    19321 ctgagaagta gaaaagaaga cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg
    19381 ttgcatgaga acggagccga ccataccgcg ccagccgata aaaccaacat ttttcataag
    19441 cgttttttcc tgcaaagatg tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt
    19501 tcaccataca aaaagcagcc aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc
    19561 agtaatgcga cttatcctgc tttgttagca cgcagaaagt ccgcggcaat tatcagggaa
    19621 tttgagttat gaatgaaatc atttctgcag cagttttatt gatcctgatt atggatccgc
    19681 tcggaaacct acctattttc atgtccgtac tgaaacatac tgaaccgaaa agacggcggg
    19741 caatcatggt gcgagagttg cttattgctc tcctggtgat gctggtgttc ctgtttgcgg
    19801 gtgagaaaat tctggcattt ctcagcctga gagcagaaac cgtctccatt tctggcggca
    19861 tcattctgtt tctaattgcc attaagatga ttttccccag cgcttcagga aatagcagcg
    19921 ggcttccggc aggtgaagag ccatttatcg tgccgctggc aattccgctg gtcgccgggc
    19981 caactattct cgccacgctg atgttgttgt ctcatcagta cccgaatcag atgggacatc
    20041 tggtgattgc tctgctgctg gcctggggcg gcacctttgt catcctgcta cagtcttcgc
    20101 tatttttacg tctgctgggc gagaaagggg tgaacgcact tgaacgcctg atgggattga
    20161 ttctggtgat gatggcaacc cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt
    20221 aaggttcaaa caggtgctgc acacaaccag acgcggcacc tgttttatta cctgaactga
    20281 taatgacctt cgcccaatcg gataaccctt ccctcttctt ctgcgcggtt cagccacaca
    20341 ctaatttgtt cgatggttaa accagtacaa gacgccagcc tttcaatact tatcgattcc
    20401 tttgcaagga tcgccagttc cgccacaaag agttgataaa aatcgacagg cgtatacgcg
    20461 ggcattactg cgtaatttgc gtcatcttca tgcagcgaaa acagttctgg ttgagcgtta
    20521 cggagtgctc tgtcatacat cagcccagga acgtctggca gtctgatcgt cacattctca
    20581 gcctgttcag cactccatga cgccccattt tctactaatc gtgaattggc tgattgcata
    20641 tcctgatttg gtttaacctg cacaggcagc cactgatgtt ttaatgcctc catcgccccg
    20701 gttatcgttc caccggtcat tcccgcacgt acaaccattg cgctttcagc aaggcaataa
    20761 atatatttat ttcgcgccat cgcattgccg acggtgaaac gtacctctgg gtaaaacggc
    20821 gaaatcaaca ccaggttgcc tgctataagc ccttcacgcc atttcactaa cgtactcgtt
    20881 tttaacaagc tatcagctaa tacgccaaca gcagttcccc cggcctccag tgccgacgcc
    20941 attgcacatt catcaatacc tcgcgcacca ccagagataa cgcaaatccc ctgttgggcc
    21001 agtttcgcgg ccagttgttg ggtatagcgc aaatcgtcag tcggagcatc tcgcgagcca
    21061 acaatcgcca taccttccgc ttgcagtaat gctttattcc cgcagccaaa taaaacggga
    21121 ggtgcatccg ttcgcaatcg gtttttcaag cgaacgggat aatcagcatc tccgcgggtt
    21181 aagatccata aaccggcgcg ttgccactta tctaccgcca acgccagact atgaccacgc
    21241 gccagcaagc caagaatacg ctcctgagaa atacgcggat cgctccagcc tgtaagtttc
    21301 tcttgcggtt gcggcaccag aagctcggcg ggactgatac gttggtgctt cagccataat
    21361 gcaaacttcc cccattcact attactaaga ggtttatact cactcgccgc cgcgcgagaa
    21421 aaatcgctgg ttagcaggag agttgcttgt gcattggctg aaagattcat caatttttta
    21481 ccgaggtaga agcaagggca agaggataaa ccgtcgggca acctgcctgg cgtaacagtg
    21541 ctgtcagaac tgtcagcgtc catgcagaat ccacgatatc gtcaaccagc aacgccggac
    21601 caggcatcaa aggaggggta atcacaaacg ccccgtcgag attttgacac tggtggaaac
    21661 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
    21721 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
    21781 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
    21841 tttccgcaca tgcctccacc agttcatcac tgaatcgccc cgtttttttc tcctgtgcta
    21901 cctgctttcc ccacccggaa tctccccaac gagaaaggat tcgccccgta gatccttgta
    21961 aactcgcagg caagttccct ttaaatccgt attgcgtaaa agccccagct gccacctgtt
    22021 tattgagatt taatggcagg tcagcgtgtt taataaataa gtttgccgca tggagtaagc
    22081 cgctatcaat gtcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaat
    22141 gttcggcacc tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
    22201 tgatatagct ttcaacgtct gccagctcac tttttctctg attcatcaaa tggttaattc
    22261 gttcatggtc aggagaaaaa ctgacaattg ttctgcgcca taatttctcg gtatacacca
    22321 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
    22381 gattcgtccg ttgttcaatg cctcgtaagg taagaccgtc attcacgcta agtacgttga
    22441 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
    22501 cacggtcttc actgccgcaa agcagtatgc caacagcaga atcgataccg cgaccggcac
    22561 gccccacctg ttggtagtaa ccaactatcg aacctggcat ctggtaatga ataacaaacc
    22621 ccaaatccgg tttgtcaaag cccattccca gcgccgttgt cgcaaccagc actttaattt
    22681 tatttgccag caacgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
    22741 cgcaagtcac gccgctgtag taagcaaatg cactgatacc atttttcctt aaccactcgg
    22801 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
    22861 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
    22921 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
    22981 ggatatcctc aacgacacgg ttattcgctg tcgcggttgt acccagaaca ggggtattcg
    23041 caggtagttg gcgcaaaata tctaatatac gtcggtaatc cgggcgaaaa tcgtggcccc
    23101 aatcagagat gcagtgcgcc tcgtcgacca caagcaagcc aatacgatcg gctataggat
    23161 ataaaaccgt ttcgataaag tcctggtttg ccaaacgttc cggggaaacc agcaagcaat
    23221 cgacctcacc ttgcagcaac ttatcgctaa tgcgctgcca ttcttctctg tttgtagaat
    23281 tcaacgtttc agccgtaata cctaaccgtt cagcggcggc aacctggtta cgcatcaggg
    23341 caagcaaagg agagatgata attgtcgggc cagcgccgcg atcgcggaag attttactgg
    23401 cgatgaagta cacagcactt ttcccccagc cagtgcgttg gacaaccagc agttttcgcc
    23461 gttggttcac caccgcgtct atggcttccc attgcccttc cctgaaagta gcggtggtat
    23521 ttcctaacat ccgctgtaaa agcaattcag ctgcgtgctt ttccatcttt atctcctgcg
    23581 atctttaggc agtgattaga acggattatt tgcaggtaaa tttgcgagag agatcaaaaa
    23641 gatgaagaga ggagaaaaga gaaatgaatt actgcgagca agctcgcagt aatttgtcgc
    23701 agtaattaac tcaacagctg gaacgcaatc atcccaacga tggcaccgac agtgccgagg
    23761 atggtttcca tcatggtcca ggttttcagc gtttcggctt cggtcgcgcc ggtaaattta
    23821 ccgaacaacc agaaaccagc gtcgttaacg tggctgacaa caatcgaacc accggcgata
    23881 caaatcgaca gcgccgccat ttgcgcaccg gagtagttca gttgttcaat aaccggcatc
    23941 accagtccca ccgccgttaa acaggcaacg gttgcagaac cctgaatgat gcgcactgca
    24001 gcggccagca cgaagcaggt gatggcaatc ggcaggccca tgccggttaa cgcttcaccc
    24061 agcgccggac ctacgccaga gtcaaccagc acctgtttga acacgccgcc tgcaccaatc
    24121 accagcagaa tgatccccgc cggttgcagc gcgtgaccgc aaatctccat cactttgtct
    24181 tttggcatgc cctgacgcat tgccaggcca taaatcgcta ccagacaagc aaccagaatc
    24241 gcggtaaacg gatgaccaat aaactcgaac cattcgtaag cggtagagcc ttccggcaca
    24301 aaacgcgcgg caatggtttt cagccccacc agcaccagcg gcagcaggat cagcgacagg
    24361 ctgaatccga aggacggcat tttgccttcg ccgagatgcg gctcgctgat gtcgtcagga
    24421 atatgcagct caacgtaacg gctgatgaag ttaccccaca gcggcccggc aataatcatt
    24481 cccggaattg ccgcacacag gccaatcagg atcatccagc caaaatcggc gttcatctgc
    24541 gatgccagca gcattggcgc tggccctggc accaggaacg ccgccgctgc cgccacgcct
    24601 gcgaataatg ggattaccag cttcaccagg ttcgtaccgg tgtggcgcgc cattgagaaa
    24661 gcaacgctaa tcagcagaac aatcgccact tcaaagaaca gcggtagcgc acagaccagc
    24721 cccgcaaggc cgatggcata atgcgcgcgg ctgtgaccga aggatttaag cattttgacg
    24781 gcaatctgat cgactgcgcc ggtttcatgt aagatcttgc caaacatagc tcccagggcg
    24841 acaaccaccg ccaggaagcc gagggtgcct cccatccctt tttccatcgt cgctgcgatt
    24901 ttatcgagcg gcataccgga aaaaaggcca gcccccatgg acaccaccat taaagccagg
    24961 aaagcgtgca tacgcgcctt catgactaaa aacagcagca gtaaaacaga ccctactgct
    25021 gttaaaacaa gcgttaatgt agtcactact tatttgcctt ttttaataac ctcaatggtg
    25081 cttgccacaa caccttccag cggttgatcg atatccacca ccagtacatc ggtttcgtcc
    25141 gcacccggct cctgcagcgt ttcaaactgc gtcaccaaca tttgggtttt aaagaaatgg
    25201 cctttgcgcg ctttcaggcg gctttcaatc acatcaaaat cgcctttcag gtagatgaaa
    25261 gagagattcg gattaccttc acgcagcaag tcgcgatagt gttttttcaa tgcagaacag
    25321 acgatcagcg acactttatt agtgcgctgc atagcaaacg cggcgtcgtt cagcgcctgc
    25381 aaccacggtt tgcgatcgtc gtcattcagc ggttcgccag acgccatttt ttcgatattg
    25441 cgccgtggat ggaggaaatc gccatcaaga aacgcggcat gaagttgatg cgccacttca
    25501 cttgcgaccg cagatttgcc gctgcccgat acgcccatca agacgtaaat gtggtgatca
    25561 tggttagtcg tgctcaaagt ggtgccccca caatacaaga attaaaaatg ttacgggtaa
    25621 ctgttatcgg taacattctc cggccggaca atatcagaag caatatccat ccgtgcctta
    25681 agtgtataag tgtgagctac ttcaaatttg tgggcttaaa tagatccgcc cggtgacaag
    25741 gtgaaaccta aatctaacat tttcggtgtc acagattcgc cacgaatacg cgccagaagg
    25801 cgttcagcgc caatactgcc catccgctca cgcggcgtca gcacgctcgc aagtcgtggc
    25861 tccatcacct gaccaatgtc atgaccgtgg aaaccggcaa tcgccatatc gtcaggaact
    25921 tttaacccca gacgctgaca ttcaaacgcc gcgccgaccg ccagatcatc attagtacag
    25981 aacacgccat ccagctgcgg atattcccgc cgcgcctggc gaatcagttc aataccggaa
    26041 gagtaagaag aagattgctc aaccatcacg ctatacggca ccaggcctgc atcccgcatc
    26101 gcctgttcgt atcccttctg tttgatgata gtacgttcgt cgagacgtgc gccgagatag
    26161 gcaatatggc gatgcccgcg agcaataatg gcggcggtca tctggcgtgc cgcttcaaag
    26221 ttatcaaaac cgacggcaat atcgaggcat ggcgacttgc tgtccatcag ttccaccacg
    26281 ggaatccccg ccacttcaat catctttaag gtgcgcggcg tgtgggtacg ttcggtgagg
    26341 ataagtccgt cgatattcca tgagagcatt gattcgagac gttcttgctc catttccggt
    26401 ttataaccgt agtgggccag catggtctga taaccgtgcg cgtcggtgac gctttcgatt
    26461 ccgcgtaata cttccgcgaa aacctggttg gtgagagaag gtaacaggac gccaatcgcc
    26521 cggctggtgg cgttagagag gatatcgggc gcacgattgg gaatatagcc cagttcatca
    26581 agagccgcgg caatcttgcc gcgtagagcg acggaaacct gctccgggtt gcgtaaaaaa
    26641 cggctgaccg tcattttggt cacgcctaca cggtcagcca catcctgaag tacgggtctt
    26701 ttctttttca tcgtcctgaa ggtacaaaag agatagattc tcttagttta acacggacgc
    26761 atcagaacct tcccggggaa aacagtgctt gtttacaatt attagatata cactaaatca
    26821 attgcatcgc attgtgctaa cgacgcggca aaaccccgga agcgtacatc agcacgcgac
    26881 cggagcgagc gaggaagcct gcaaaagggc aggcttcaaa gatgccgttg agttttaaac
    26941 cggcggcaga tcgaacagta acacttcgct gtcgctatcc gcatggatgg agattgcctg
    27001 ctcatcccag attgccagac catcgctggt cgaggctttc acgccgttaa tggtgacatt
    27061 gcctttcacc acctggatcc agacgcggcg ttcagcggca atctgatgca ccgactgctc
    27121 atctttcagc aacgcccagc ggtacagttc catatcctga tgcactttca atgaaccatc
    27181 tcgcgcatct ggcgaaagca ccagttgttt gccctgcacg gcatcgaagc gacgctgttc
    27241 ataacgcggc gtaataccgt tttcttcagg catgatccag atctgataca gatgcagacg
    27301 ctcggtgctg cttgggttgt actctgagtg acgaataccc gtacccgcac tcataatctg
    27361 gaactcgccc gccggaacct gctctttatt gcccatgctg tcctggtgct caacagtacc
    27421 ttccagcacg taggtcaaaa tttccatatc tttatgcggg tgagtgccga agccctgccc
    27481 tgcttcaatc acgtcgtcgt taatcacgcg cagcgcggag aagcccataa agttcggatc
    27541 gtaatagttg gcaaaagaga aagtatgcca ggagtccagc cagccatgat ttgcatgacc
    27601 acgttcattt gctttgcgta agtagatcat ttgtattcgc cccctgaatg atttcgatgg
    27661 gcttagtgtg gacccgatct gcctgggatg atagagggtg aaaattgacc cctctgttca
    27721 aaaaaattga actatttgag gggccatcaa acttacttcg cgagagtcac ggtggaggga
    27781 gaggcttgct cgaatccgcg ttcaaggatt tccagattcg taagaacttc agattccttg
    27841 acgtaatttg gcgcaccgtg ggtgatggtt tgatacaacg catcataaac gcgcccgtaa
    27901 tcgcccatct ccggcttcat ctcttctctg accgtcacgc cctcgtcatt gacatactcc
    27961 agcacaccga ccgaatcatc cgctgcgaat cccggttcgc ccggcataat attagccttc
    28021 aggctggttt cctgctggtc gataccgtat ttaataaacg aacctttctt accgtgaacg
    28081 ataaatttcg gataatcgat tttcaccaga tggctggttt tgacgatggc ttttagatcg
    28141 ccataaaaca gttgcgcttc gaaagtatcg tcaggattgg ctttattgcg caggctgcgg
    28201 atgtcataag cgacgtgatc cgggcgaccg aacagagaaa taatctggtc catcgtatgc
    28261 acaccaaggc catagaacgc gccatcctgc ggcagcccag gtttggtttc tgccaccggg
    28321 cggtaatagt caaaatggct ttccacttcg acaatctctc ccagcttgcc gctttcaatc
    28381 gcttttttcg ctgtcaggaa gcaggaatca aagcgacgat tctgatatgg cgtgacggtc
    28441 agccctttgc ttttcgccag tgcaaacagc tctttcgcct gcgcaagtgt cggagtgaac
    28501 ggtttttcga ccagcacatt tttcccggct tccagcgcgc gtttcgcgta ctcgaaatgg
    28561 ctgtccgcgt gggtgcagac cacaaccagc ttaacatcgg gatcgtttag cacttcgtcg
    28621 agatcgctgg tgaaatggat atgagagtaa atgggagcct gttcttccgg cttcgcatgg
    28681 cgacgaaaaa tatgcgcgac atgccagcta tccttgcggt taagtacata cggcagatgg
    28741 taacgggtgg tgcttttgcc gaagccaata aaggcgcagt tgatgaccat gatttcgtcc
    28801 tttttaaggt ggttatttac accttagcgc aaagcggacg tggttcctac tggagtgcgc
    28861 ataacgaaca caagcacttc ccgaggataa attgagaacg aaagatcaaa aaaaaagcca
    28921 gcacccggct ggctaagtaa tactggaagc aatgtgagca atgtcgtgct ttcaggttct
    28981 ccgcgagggt cttcctgatc gcgagacaat aataatcatt ctcattcgca cttgtccaac
    29041 actttttgca aaaaaaatgc atttgactcg cattcgaaag tcaatgatgt tgaaagggac
    29101 atttacccca aagaggacaa aggaatgagt gagatagtga tacgccatgc tgaaacgcgc
    29161 gattacgagg ccatcaggca gattcacgcc cagccggagg tgtattgcaa cacattacag
    29221 gtgcctcatc cttccgatca tatgtggcag gagcgactcg ccgatcgtcc cggcatcaaa
    29281 cagttagttg cctgtattga tggaaccgtc gtgggtcacc tcaccattga cgtgcaacag
    29341 cgcccacgcc gcagtcatgt tgccgatttt ggtatttgtg tcgactcccg ttggaagaat
    29401 cgcggcgtcg ccagcgccct tatgcgagag atgattgaaa tgtgcgacaa ctggttgcgg
    29461 gtagatcgta tcgagctaac ggtgttcgtc gataacgcgc cagcaattaa ggtctataaa
    29521 aaatacggct ttgaaattga agggactggt aagaagtacg cattgcgtaa tggtgaatat
    29581 gtcgatgcgt attatatggc gcgggtgaag taaagaatat tctcaaattg cctgttacca
    29641 atgtgaataa ttagttttgt tcatctaatt tgagtttttc cttatatt
//
LOCUS       DAZKFU020000032        28965 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZKFU020000032 DAZKFU020000000
VERSION     DAZKFU020000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.032"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            321..695
                     /gene="tomB"
                     /locus_tag="ACHOQQ_RS05705"
                     /old_locus_tag="pgaptmp_001141"
     CDS             321..695
                     /gene="tomB"
                     /locus_tag="ACHOQQ_RS05705"
                     /old_locus_tag="pgaptmp_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGU4469418.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            721..939
                     /gene="hha"
                     /locus_tag="ACHOQQ_RS05710"
                     /old_locus_tag="pgaptmp_001142"
     CDS             721..939
                     /gene="hha"
                     /locus_tag="ACHOQQ_RS05710"
                     /old_locus_tag="pgaptmp_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HGU4469419.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            1111..1662
                     /gene="maa"
                     /locus_tag="ACHOQQ_RS05715"
                     /old_locus_tag="pgaptmp_001143"
     CDS             1111..1662
                     /gene="maa"
                     /locus_tag="ACHOQQ_RS05715"
                     /old_locus_tag="pgaptmp_001143"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGU4469420.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            1778..2248
                     /gene="ylaC"
                     /locus_tag="ACHOQQ_RS05720"
                     /old_locus_tag="pgaptmp_001144"
     CDS             1778..2248
                     /gene="ylaC"
                     /locus_tag="ACHOQQ_RS05720"
                     /old_locus_tag="pgaptmp_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGU4469421.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2412..3962
                     /gene="pdeB"
                     /locus_tag="ACHOQQ_RS05725"
                     /old_locus_tag="pgaptmp_001145"
     CDS             2412..3962
                     /gene="pdeB"
                     /locus_tag="ACHOQQ_RS05725"
                     /old_locus_tag="pgaptmp_001145"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGU4469422.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(4004..4357)
                     /gene="ybaA"
                     /locus_tag="ACHOQQ_RS05730"
                     /old_locus_tag="pgaptmp_001146"
     CDS             complement(4004..4357)
                     /gene="ybaA"
                     /locus_tag="ACHOQQ_RS05730"
                     /old_locus_tag="pgaptmp_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HGU4469423.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4477..4573)
                     /gene="ffs"
                     /locus_tag="ACHOQQ_RS05735"
                     /old_locus_tag="pgaptmp_001147"
     ncRNA           complement(4477..4573)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHOQQ_RS05735"
                     /old_locus_tag="pgaptmp_001147"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4736..5047
                     /locus_tag="ACHOQQ_RS05740"
                     /old_locus_tag="pgaptmp_001148"
     CDS             4736..5047
                     /locus_tag="ACHOQQ_RS05740"
                     /old_locus_tag="pgaptmp_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGU4469424.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(5078..5650)
                     /gene="ybaY"
                     /locus_tag="ACHOQQ_RS05745"
                     /old_locus_tag="pgaptmp_001149"
     CDS             complement(5078..5650)
                     /gene="ybaY"
                     /locus_tag="ACHOQQ_RS05745"
                     /old_locus_tag="pgaptmp_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGU4469425.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5868..6728
                     /gene="tesB"
                     /locus_tag="ACHOQQ_RS05750"
                     /old_locus_tag="pgaptmp_001150"
     CDS             5868..6728
                     /gene="tesB"
                     /locus_tag="ACHOQQ_RS05750"
                     /old_locus_tag="pgaptmp_001150"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGU4469426.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKKMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6777..8063)
                     /gene="amtB"
                     /locus_tag="ACHOQQ_RS05755"
                     /old_locus_tag="pgaptmp_001151"
     CDS             complement(6777..8063)
                     /gene="amtB"
                     /locus_tag="ACHOQQ_RS05755"
                     /old_locus_tag="pgaptmp_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGU4469427.1"
                     /translation="MKIATIKTGLASLAMLPGLVIAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(8093..8431)
                     /gene="glnK"
                     /locus_tag="ACHOQQ_RS05760"
                     /old_locus_tag="pgaptmp_001152"
     CDS             complement(8093..8431)
                     /gene="glnK"
                     /locus_tag="ACHOQQ_RS05760"
                     /old_locus_tag="pgaptmp_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGU4469428.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(8612..10393)
                     /gene="mdlB"
                     /locus_tag="ACHOQQ_RS05765"
                     /old_locus_tag="pgaptmp_001153"
     CDS             complement(8612..10393)
                     /gene="mdlB"
                     /locus_tag="ACHOQQ_RS05765"
                     /old_locus_tag="pgaptmp_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4469429.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10386..12158)
                     /gene="mdlA"
                     /locus_tag="ACHOQQ_RS05770"
                     /old_locus_tag="pgaptmp_001154"
     CDS             complement(10386..12158)
                     /gene="mdlA"
                     /locus_tag="ACHOQQ_RS05770"
                     /old_locus_tag="pgaptmp_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4469430.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(12188..12646)
                     /gene="decR"
                     /locus_tag="ACHOQQ_RS05775"
                     /old_locus_tag="pgaptmp_001155"
     CDS             complement(12188..12646)
                     /gene="decR"
                     /locus_tag="ACHOQQ_RS05775"
                     /old_locus_tag="pgaptmp_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGU4469431.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(12800..13618)
                     /gene="cof"
                     /locus_tag="ACHOQQ_RS05780"
                     /old_locus_tag="pgaptmp_001156"
     CDS             complement(12800..13618)
                     /gene="cof"
                     /locus_tag="ACHOQQ_RS05780"
                     /old_locus_tag="pgaptmp_001156"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGU4469432.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            13718..15418
                     /gene="ybaE"
                     /locus_tag="ACHOQQ_RS05785"
                     /old_locus_tag="pgaptmp_001157"
     CDS             13718..15418
                     /gene="ybaE"
                     /locus_tag="ACHOQQ_RS05785"
                     /old_locus_tag="pgaptmp_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HGU4469433.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15483..16178
                     /gene="queC"
                     /locus_tag="ACHOQQ_RS05790"
                     /old_locus_tag="pgaptmp_001158"
     CDS             15483..16178
                     /gene="queC"
                     /locus_tag="ACHOQQ_RS05790"
                     /old_locus_tag="pgaptmp_001158"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGU4469434.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLTDKP
                     TVMAAMKQKTGLK"
     gene            complement(16230..16628)
                     /gene="fadM"
                     /locus_tag="ACHOQQ_RS05795"
                     /old_locus_tag="pgaptmp_001159"
     CDS             complement(16230..16628)
                     /gene="fadM"
                     /locus_tag="ACHOQQ_RS05795"
                     /old_locus_tag="pgaptmp_001159"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGU4469435.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(16734..17105)
                     /gene="ybaV"
                     /locus_tag="ACHOQQ_RS05800"
                     /old_locus_tag="pgaptmp_001160"
     CDS             complement(16734..17105)
                     /gene="ybaV"
                     /locus_tag="ACHOQQ_RS05800"
                     /old_locus_tag="pgaptmp_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGU4469436.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(17256..19127)
                     /gene="ppiD"
                     /locus_tag="ACHOQQ_RS05805"
                     /old_locus_tag="pgaptmp_001161"
     CDS             complement(17256..19127)
                     /gene="ppiD"
                     /locus_tag="ACHOQQ_RS05805"
                     /old_locus_tag="pgaptmp_001161"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4469437.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDKVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(19319..19609)
                     /gene="hupB"
                     /locus_tag="ACHOQQ_RS05810"
                     /old_locus_tag="pgaptmp_001162"
     CDS             complement(19319..19609)
                     /gene="hupB"
                     /locus_tag="ACHOQQ_RS05810"
                     /old_locus_tag="pgaptmp_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGU4469438.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(19800..22154)
                     /gene="lon"
                     /locus_tag="ACHOQQ_RS05815"
                     /old_locus_tag="pgaptmp_001163"
     CDS             complement(19800..22154)
                     /gene="lon"
                     /locus_tag="ACHOQQ_RS05815"
                     /old_locus_tag="pgaptmp_001163"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGU4469439.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(22342..23616)
                     /gene="clpX"
                     /locus_tag="ACHOQQ_RS05820"
                     /old_locus_tag="pgaptmp_001164"
     CDS             complement(22342..23616)
                     /gene="clpX"
                     /locus_tag="ACHOQQ_RS05820"
                     /old_locus_tag="pgaptmp_001164"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGU4469440.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(23742..24365)
                     /gene="clpP"
                     /locus_tag="ACHOQQ_RS05825"
                     /old_locus_tag="pgaptmp_001165"
     CDS             complement(23742..24365)
                     /gene="clpP"
                     /locus_tag="ACHOQQ_RS05825"
                     /old_locus_tag="pgaptmp_001165"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGU4469441.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGKSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(24611..25909)
                     /gene="tig"
                     /locus_tag="ACHOQQ_RS05830"
                     /old_locus_tag="pgaptmp_001166"
     CDS             complement(24611..25909)
                     /gene="tig"
                     /locus_tag="ACHOQQ_RS05830"
                     /old_locus_tag="pgaptmp_001166"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU4469442.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFSIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(26253..26570)
                     /gene="bolA"
                     /locus_tag="ACHOQQ_RS05835"
                     /old_locus_tag="pgaptmp_001167"
     CDS             complement(26253..26570)
                     /gene="bolA"
                     /locus_tag="ACHOQQ_RS05835"
                     /old_locus_tag="pgaptmp_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGU4469443.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            26875..27453
                     /gene="yajG"
                     /locus_tag="ACHOQQ_RS05840"
                     /old_locus_tag="pgaptmp_001168"
     CDS             26875..27453
                     /gene="yajG"
                     /locus_tag="ACHOQQ_RS05840"
                     /old_locus_tag="pgaptmp_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4469444.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            27497..>28965
                     /gene="ampG"
                     /locus_tag="ACHOQQ_RS05845"
                     /old_locus_tag="pgaptmp_001169"
     CDS             27497..>28965
                     /gene="ampG"
                     /locus_tag="ACHOQQ_RS05845"
                     /old_locus_tag="pgaptmp_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGU4469445.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHL"
BASE COUNT         6910 a         7821 c         6966 g         7268 t
ORIGIN      
        1 ttctttattc attcctcggt tattacgtca tattcagagc aatcctggta ttagtgtcac
       61 caatttcatc tggcgataat cctgaaatgt tatgaacagt tcgagcaaac tgcttttacc
      121 tgctgcgggt tggtgctagt atgaaaaagt gagccctgtc ccgcttcctt cctaattgta
      181 atttttcgta ataatgcgat gaaaacctgc aaagagtggc ttatagttaa gctaacaaac
      241 gagagggcaa gtccaggtca gtaagttttt tccatcccga aaggtgtccg ttagttcaac
      301 cgctaagaag gggacgcgtt atggatgaat actcacccaa aagacatgat atcgcacagc
      361 ttaagtttct ctgtgaaact ctgtatcatg actgccttgc aaaccttgaa gaaagcaatc
      421 atggctgggt taacgaccca acctcggcga tcaacctcca gttgaatgaa ctgattgagc
      481 atattgcgac cttcgcactt aattacaaaa ttaagtataa tgaagacaat aagctcattg
      541 agcagatcga cgaatatctg gatgacacct ttatgttgtt cagcagttat ggtattaata
      601 tgcaggatct tcagaaatgg cggaagtcag gtaatcgact attccgttgt tttgtcaatg
      661 cgacgaaaga gaatcctgcg agtttatctt gttagaatta ttacaaccat gggtagaagt
      721 atgtccgaaa aacctttaac gaaaaccgat tatttaatgc gtttacgtcg ttgccagaca
      781 attgacacgc tggagcgtgt tatcgagaaa aataaatacg aattatcaga taatgaactg
      841 gcggtatttt actcagccgc agatcaccgc ctcgccgaat tgaccatgaa taaactgtac
      901 gacaagatcc cttcctcagt atggaaattt attcgctaat aaataattcg ctttcggagc
      961 tataaccggc tgtttattaa gaattttata ctttttcgcc atgaagacat accctatgtg
     1021 atctttatca cacagatgta atgggaacgt tctcttcact gacttttcgt cttactgtgt
     1081 tgccgcattt tcagcaaccg gaggtcagta atgagcacag aaaaagaaaa gatgattgct
     1141 ggtgagttgt atcgctcggc agatgagacg ttatctcgcg atcgcctgcg cgctcgtcag
     1201 cttattcacc gatacaatca ttccctggcg gaagagcaca cattacgcca gcaaattctc
     1261 gctgatttat tcggtcaggt gacagaggct tatattgagc caacgtttcg ctgtgactat
     1321 ggctataaca tttttctcgg taataatttt ttcgccaact tcgattgcgt gatgcttgat
     1381 gtctgcccta ttcgcatcgg tgataactgt atgttggcac caggcgttca tatctacacg
     1441 gcaacacatc ccatcgaccc tgtagcacgt aatagcggtg ctgaactggg gaaacccgtc
     1501 accatcggta ataacgtctg gattggcgga cgcgcggtca ttaaccctgg tgtgaccatt
     1561 ggtgataacg tcgtggtagc ctcaggtgca gttgtcacaa aagatgtccc ggacaacgtt
     1621 gtcgtgggcg gtaatccagc cagaataatt aaaaaattgt aatcggtttt tcgcaactgt
     1681 tatgcaaaat tgtggtaaat ctgttacttt ccctctacta ttcccacgtt aaaatagggt
     1741 gttccctgga aagttgcaga taccacgaag gcaaacgatg accgaaatac aacgcctgct
     1801 gaccgaaacg attgagtctc tgaatacccg cgaaaaacgc gacaacaaac cccgctttag
     1861 tatcagtttt atccgtaaac atccggggct gtttatcggt atgtacgttg ctttttttgc
     1921 caccctggcg gtgatgttgc agtccgaaac gctttcaggc tctgtctggc tactggttgt
     1981 attatttatc ctgcttaatg gtttcttctt tttcgatgtc tacccacgct accgctatga
     2041 agatatcgac gtgctggatt tccgcgtttg ctataacggc gaatggtaca acacgcgctt
     2101 tgtacctgcc gcgctggttg aagccatctt gaactctccg cgtgtcgcgg atgttcataa
     2161 ggaacaactg caaaaaatga tcgtccgtaa aggtgaattg tctttttacg atatttttac
     2221 cctcgctcgc gccgaatcaa catcttaagt taggattaca taccaggcgt aaagctctgc
     2281 gcctggtcaa atgacaacga tcgtttccac ccatcacttc atgaaatacc agctctacct
     2341 ccttatctcc tgccagcctt tttccacaat cagatatact ttccctacac tgtgttaata
     2401 aggatatgct ggtgagaaca cgacatctgg tcggccttat ttcgggagta ctgattcttt
     2461 cagtattgct gcctgtcggc ttaagcatct ggctggccca tcagcaggta gaaacatcgt
     2521 ttattgaaga gctggatacc tattcctccc gcgtcgctat tcgggccaat aaggtggcga
     2581 cacaagggaa agatgcgctg caagagctgg aaagatggca aggcgctgac tgtagcgaag
     2641 cccatctcat ggaaatgcgt cgggtatctt acagttatcg ctatattcag gaagtggttt
     2701 atatcgataa caacgttccc caatgttcgt ctctggagca tgagagtccg cccgatacct
     2761 tccccgagcc aggtaaaatt tcgaaagatg gttatcgtgt ctggttaaca tcgcataacg
     2821 atttaggcat tatccgttac atggtcgcca tgggaacggc acattatgtc gtcatgatcg
     2881 accccgcttc ctttattgat gtcattccct atagctcatg gcaaattgat gccgccatta
     2941 ttggcaatgc ccataatgtt gtcataacca gcagcgatga aattgctcag ggaattatta
     3001 ccaggctaca aaaaacaccc ggtgagcata tcgaaaataa tggaatcatt tacgatatcc
     3061 tgcccttccc ggagatgaat atttcgatca tcacctgggc ttcaacgaaa atgttgcaga
     3121 aaggctggca tcggcaagtc tttatttggt taccgctcgg gttggtgatt ggcctgctgg
     3181 cagcgatgtt tgtgctgcgt attttgcgcc gcatccagtc accgcatcat cggctgcagg
     3241 atgctatcga aaatcgtgat atttgcgtgc actatcagcc gattgtctcc ttagccaatg
     3301 gcaaaattgt cggtgctgag gcactggcgc gctggccgca gacagacggc agttggttgt
     3361 caccagacag ttttattccg ctggcacagc aaacgggcct ttctgagcca ttgacgctac
     3421 tgattataag aagcgtcttt gaagatatgg gcgactggct gcgtcagcat ccacagcagc
     3481 atatttcgat caatcttgaa tccaccgtgc tcacctcgga aaaaatcccg caattgctgc
     3541 gtgaaatgat caatcactat caggttaatc ccagacagat cgcgcttgaa ctcactgaac
     3601 gcgagtttgc cgatccgaaa accagcgccc cgataatttc tcgctaccgg gaggcgggcc
     3661 atgaaattta tcttgatgat tttggtacgg ggtattcaag tttaagttat ttacaggatc
     3721 tggatgtcga cattctgaag atcgataaat ctttcgttga tgcgctggaa tataaaaatg
     3781 tcacgccgca tatcatcgaa atggcaaaaa cactgaaact gaaaatggta gcggagggaa
     3841 tcgaaaccag taaacaagaa gagtggctac gccagcatgg cgtgcactac ggtcagggct
     3901 ggctctacag caaggcatta ccgaaagaag atttcttacg ctgggccgag caacatttgt
     3961 gaatttttgg gctgacggca cgccgcacag cccgtcacgc tacctattcg tcgatgattg
     4021 actcgaatcc gccatagatc attcgcttgc cgtcaaacgg catggactcg ccgaactctt
     4081 tcatccgtgg gtccgacatc atcttttgat tagctgcgtc gcggacctct tttgaagggt
     4141 attcaatcca gctaaagaca acctcttcat tctcttccgc tttcaccgcc atacgaaaat
     4201 cggtcacttt gccatccggt acatcgctgg cccagcattc gacaatacga agcgcgccaa
     4261 actctttaaa caatggcgcg gccttagcgg ccatttcccg ataggcatcc tttttatcgg
     4321 caggaacggc aaccacaaaa ccatcaacat acttcatcag acatacctcc gaagcgtact
     4381 gcgtagccag acaattcttg cgacgtcagt tgtggttaaa gtttagtcac gctgggattt
     4441 tttgacgaaa cggtgggaag cagaaatggg tgggggccct gccagctaca tcccggcaca
     4501 cgcgtcatct gccttggctg cttccttccg gacctgacct ggtaaacaga gtagcgttgc
     4561 gggagaacca acagagcccc cattgagagc gttgagaacc aacgcgcagg cgcattatca
     4621 ctgctgcccc ggctaattgc aacccattgc gtgccagatg ctggtttctt gcgcaatgcg
     4681 acttcactcg ggcgtttttc cagattatgc tgaaaaatta cctcaggaag aaaagatgga
     4741 aaaagaagat tcatttcccc aacgcgtctg gcaaatcgtc gccgctattc ccgaaggcta
     4801 tgtaaccact tacggtgatg tggcgaaact ggcgggatcg ccccgcgccg cgcgccaggt
     4861 gggcggtgtg ttaaagcgtc tccctgaagg cagcacctta ccctggcacc gggtggttaa
     4921 tcgccacggc acaatttcgc taaccggacc ggatttacag cgtcagcgac aggcattact
     4981 ggcagaaggt gtgatggtat cgggaagcgg gcaaatcgac ttgcagcgtt atcgctggaa
     5041 ctactaaccc tctccgggcg gagagggtta acgtcgctta atattgcgta ggagctggaa
     5101 ctgccgaaga cggattcacc tgagttggtg aagtcgaagg tacggtagtc gttgcgccac
     5161 cgctggcctg aaccggcacg gcagtttgct gtaccggcac caatgtcagg tcggctttag
     5221 ttccgccctg gttgatcacc ggctgaacgg tatcggtgat aaataccagt ttgtcattca
     5281 cggtaatcgc cgcactcaac agaatacgcg cgttcggctg aacatctgcc gggttaaatg
     5341 gcagaacaaa gctgaatggt gactgtttac cttcagtacg caccgctttc tgcgccagca
     5401 cttttgacgg tgcatcggct aacgacgcgt cagaaagtgt cacggtcagc acagcatcag
     5461 gcggcagtgc gactttctga cggatccaga cggtaccgga gacattcggt tgctggatag
     5521 caggttgttg tgttgctgaa atagacgtat ttgcagccgg ggctggcgtc tgaatatccg
     5581 cgcttttatc tgcgcaagcc gccaacgcaa tcgcaaccgc taaaccactg gccatgtgca
     5641 cgagtttcat tcatttctcc ttattatcaa tgcaccagcg ggctaacttt cctcgccgga
     5701 agagtggtta acaaaatagt aacgtcaaca agtgtggcac acatcacgca tttctgcctg
     5761 taattagccc gtaattcaga ccattgcacc catcggacca gtagcaaatt tgcgttatac
     5821 tcaactcact ttggcttgct gcggcagctt tgttactgga gagttatatg agtcaggcgc
     5881 taaaaaattt actgacattg ttaaatctgg aaaaaattga ggaaggactc tttcgcggcc
     5941 agagtgaaga tttaggttta cgccaggtgt ttggcggcca ggtcgtgggt caggccttgt
     6001 atgctgcaaa agagaccgtc cctgaagagc ggctggtaca ttcgtttcac agctactttc
     6061 ttcgccctgg cgatagtaag aagccgatta tttatgatgt cgaaacgctg cgtgacggta
     6121 acagcttcag cgcccgccgg gttgctgcta ttcaaaacgg caaaccgatt ttttatatga
     6181 cagcctcttt ccaggcacca gaagcgggtt tcgaacatca aaaaaaaatg ccgtccgcgc
     6241 cagcgcctga tggcctgcct tcggaaacgc aaatcgccca atcgctggcg cacctgctgc
     6301 cgccagtgct gaaagataaa ttcatctgcg atcgtccgct ggaagtccgt ccggtggagt
     6361 ttcataaccc actgaaaggt cacgtcgcag aaccacatcg tcaggtgtgg atccgcgcaa
     6421 atggtagcgt gccggatgac ctgcgcgttc atcagtatct gctcggttac gcttctgatc
     6481 ttaacttcct gccggtagct ctacagccgc acggcatcgg ttttctcgaa ccggggattc
     6541 agattgccac cattgaccat tccatgtggt tccatcgccc gtttaatttg aatgaatggc
     6601 tgctgtatag cgtggagagc acctcggcgt ccagcgcacg cggctttgtg cgcggtgagt
     6661 tttataccca agacggcgtg ctggttgcct cgaccgttca ggaaggggtg atgcgtaatc
     6721 acaattaaaa aaatagccgg aggtgaaaac cgtccggctg ttttttgcag tgcttgttac
     6781 gcgttatagg cattctcgcc gtgactgttg acgtccagcc cttctcgctc ttgttcttcc
     6841 ggcacacgca ggccaaccgt cagatccgcc agtttgtagc caataaatgc caccacaccg
     6901 gaccagacga tcgtaatggc aatgctttcc aactgtacca gcaactgatg gcccatcgtc
     6961 acaccttcag cgaagcccac gccgcccagc gaactggcgg caaaaatccc ggtcatgata
     7021 cagccgacaa tgccacaaac gccgtgcaca ccgaagacat cgcagggatc atccacccgc
     7081 agcaagcgtt tgagcatggt aacgccccac aagcccgcca gaccagctac cacgccgata
     7141 atcaacgcgc cgccaacccc aatgtagcca caggctggcg tcacgccgac cagaccggca
     7201 atcgcgccag aacacgcccc cagcagtgaa ggcttaccac gcagtgccca ttcaccgaag
     7261 atccagccaa gaattgcagc cgccgttgcg accacagtat tcacaaaagc cagtgccgcg
     7321 atttcattcg ccgtgcccgc tgacccggcg ttaaagccaa accaaccgat atagagaatg
     7381 gcagtcccgg tgaagaccat cggcaggttg tgtggtttaa acgcctcttt accgaagccc
     7441 acgcgttttc ctatcagata cgcgcccacc agaccggcga ttgcggcgtt aatgtgcacc
     7501 accgtgccac ccgcgaaatc cagcgcaccg tgagaagcca gcaaaccacc gccccagacc
     7561 atatgcgcaa ttggaatgta agagagcgtc agccatacca ccacgaaaat caacacagct
     7621 gagaagcgga ttcgttccgc cagcgcccca actatcaagc ctacggtaat gcaggcaaac
     7681 gatccctgaa acgctacgtg gatatactga taaatgctgc ccatcaccgc cgtcagttcg
     7741 atgtttttca gcatcagcca gttaatgttg ccgaagaagt tgttgccctc accaaacgcc
     7801 agcgagtaac cgtaaaccac ccagagaata cagaccagtg caaacgtcac cgtcacctgc
     7861 gtcagcatcg acagcacgtt tttgccgcga atcaacccac cgtaaaacag ggcaatcccc
     7921 ggaatagtca taaacagcac cagcgcagta caaatcatca taaacgcatt gtcggcttta
     7981 tcggccaccg caggtgcagc tattaccagt cccggcagca tcgccagtga agcaagccca
     8041 gtttttatcg tcgctatctt catttttcgt tcctgttgct gtgtgccaga gattacagcg
     8101 ccgcttcgtc ggcttcgccg gtacgaatac gaatgacgcg ttgcaattca gcgacgaaga
     8161 ttttgccgtc gccaattttt ccggtgtaag ccgccttact gacgatatcg atcacttcat
     8221 cgagttggtc atcagcaatc gccacatcaa tttttacttt tggcaggaag ttgacgctgt
     8281 attccgcccc ccggtacagc tcggcatgcc ctttctgacg cccgaaacct ttcacttcgg
     8341 tgacggtcag gccctgaata ccaatggaag ataacgcttc acgaacgtct tccagcttga
     8401 atggtttgat tatcacggtc accagcttca tagatcccct ccggtcagaa ttcggtaatg
     8461 gttctgctac acgaagaagg tattgcaagc ggtgtgccag aaatgaaaaa cgagaaagag
     8521 agcaggaagt taagaagatt gaaaatgaaa acgcactatg acagtgcaca gtgcgttaca
     8581 tttttgcacc aacgatgaaa tattgcgcta ttcaggcgct caatgattct tcttcacgca
     8641 cgctggctgc cagctcttcg cccgcaagtt gcagttgata catctgccag tagcgtccct
     8701 gggccgccag cagttgctgg tgagtgccct gctccacggc ttgcccacga tgaagcacca
     8761 gaatagtgtc ggcatcaaca atggtcgata agcggtgagc aatcaccacc agcgtggtat
     8821 gttcacgcac cgccgccaga gcatgttgaa tcgcctgttc agtaccggag tcaatgctgg
     8881 cggttgcctc atcaaggatc aggatttgcg gcgtctccac cagcacgcgc gccagtgcca
     8941 gcagttgctt ttgcccgact gagagattat ttccctgctc gcccagcggc gtgtaaatac
     9001 cgtcgctcat gctacgcgcc agctccgcca gttgcacggt ttccagcgcc tgccagacgc
     9061 gttcttcgga gatatcacgc cccagcgtca cgttggcgag gaaggtatcc gccagcacca
     9121 ccggatcttg ctgcaccatt gccacgccct ggcgcagcgc gctgtgactt agcgaactta
     9181 atggacgacc atcaaggcga atctcacctt ccgttagcgg gtaatagccc atcaataaac
     9241 tggcgagggt acttttacca ctgccggtat gcccgaccag cgccacaaag ttacgcgaag
     9301 gcacagagag attaatgttc tttagcacca gattgtcatc gcgataagca aatgacacgt
     9361 tatcgacttc gatggtgccg ctctgtaacg ggcgatcatc attgccatat tgctggcgcg
     9421 gtccgtccat cagctcaaac acgcgctcac cagcaacaac agcctgttgc agcatcgcct
     9481 gctgcgtggt cagttcgatt aagggttcgt taagtcgccc aagatagctg ataaacgcat
     9541 acaacacgcc cacttcaatg gtgccgctgg cggagaagcc aaacagcatc aacaagccac
     9601 agagaatgag cgatgaaaac agactcagca gcggacgcag gagaaaaccg tcgaggcgca
     9661 gggtttgcat cctcgccata tagtgtgaac gactggcctc acccatgcgt tcgccaaatc
     9721 gcgcctgctg gcggaactgc tggataacgc tcatgccatt gatgatttcg ttaaagccgt
     9781 cgttgatatc cgccagatag gcgcgcacac gacggacaat cggcgtgctg taacgctggt
     9841 atatcaccat taccaccagc accaccggga aaatcattat cgccaccagc gccattcgcc
     9901 agtcgaggct gaacatcgcc accagcatcg cgcccaccag tgcggcactg cgcaggacag
     9961 ttgccactac ggtaacgtag agatcgcgga tcacttcagt gtcattagtg acgcgggaaa
    10021 tcacctgccc gacgggttgg gtatcaaact cgcttaatgg ctggcgtaac gcagcatcca
    10081 tcacgtcggt acgcaactgt tgcactacgc ctactgccgc ccgattaaac agcagcgact
    10141 gcgcgtaatg tagcccggcg gcaaacagtt gtagcccaac atacgccgca gccagccctg
    10201 caaccacttt caacggcagg ttatttttcg ctaccatatt gtcgataaaa tagctgataa
    10261 gcagcggccc actgacttct gccgctgccg caacccacat catcaggacc gcaatcccca
    10321 gcggtttacg ccacggcgaa ccgtacgcta acagacgctt gagagtcggc cacagttggc
    10381 taaaactacg catcgacggc ctcctcgcga atttccggag cgtcgtcgag cgccgcctcc
    10441 agttgttgat agcgatacat atcgcgatac cagccacttt gttgcgccag ctcatcatga
    10501 ttgccacgct gggcgatatg tccgtgctgc atcacaataa tttcactggc ttccgtcagt
    10561 gcagaaaggc gatgggcact gatgattacc gttcttccct gcccccactg acgcaggtta
    10621 tgcaggatct ggtgctctgt gcgtccgtcc accgccgaaa gcgcatcatc aaggatgagg
    10681 atttccgcgt tgactaataa cgcacgagca atggagatac gctgtttttg cccgccggaa
    10741 agcatcacac cgcgctcgcc cacctctgta tcgtaacctt gcggtagacg caaaatatcg
    10801 tcatgtacgc tggctaaccg cgcgacatgc tcaatctctt gctgggtggc attcgggcaa
    10861 cccagcgcga tgttattcgc cacggtgtca gaaaaaagga atggcgtctg gctaactacc
    10921 gccaggcggc tacgccagct atcgagttgt aacttcgtca gaggaatatc atgaaagcga
    10981 atatccccct cgctgacgtc gaaatgacgc tgaatgagcg acaacagggt acttttgccg
    11041 gaaccagtcg gcccgcagat acccagcatc tgaccgggtt tcagggcgaa attgacgttt
    11101 tccagcgcag gatggtcagt ctgcggatac gtgaactggt gaatatttac atccagttcg
    11161 ccacgccctt ccggcacggg ttcgctacca tcgttcacca ccggcgcttc cgccagcatc
    11221 gcgcgaatac ggctgtacgc agcactacca cgttccacaa tgttaaacat ccatgccagc
    11281 gccagcattg gccaaatcat cagacctaaa tacatcataa aactggtgag ctggcccagc
    11341 gttaaactgc cctgcaccac catccaacta ccgccgccaa tcgccagcaa gttcgccata
    11401 ccaatcgcga tatagatggt cgggtcgaaa cgagcatcaa tacgcgccac ccgcatgttt
    11461 tttttgccgg tatcttcggc atccgcggca aataacgccg actggcgatc ttccagacca
    11521 aaggctttga tcatgcggat actggtgagg ctttcctggg tgcggtcatt aagactggaa
    11581 aacgccgcct gtgccagctt aaagcgttca tgcaaagcat cgccgttgcg cttaatcatg
    11641 atcgccatca ctggcatcgg taacagggca aataaggtca actgccagct aatttgcgta
    11701 gacatcataa tcaacacagc gcagcccatt accagtgaat ccaccagcgt cagcacccct
    11761 tctccggcgg caaacacgac gcgatcgacg tcattagtcg cacgagccat gaggtcaccc
    11821 gtgcgatgac gcaggtaaaa ctcaggatgc tgccggctta gctgacggta ataatcttca
    11881 cgcagttcaa cagccagttg ataagacgca ccaaacagca atacgcgcca gacgtaacgc
    11941 aggagataaa ccacaacggc aatcagcacc atggtggcga tccacatcag gatctgcccg
    12001 gtagtaaagt gttgttctgt cacgccatcg acaacaatac caaccacttt tggtggaacc
    12061 agttgcagca tcgcgataat gacaagcaag gcgacagccc cgagatagcg acgccattcc
    12121 cgacggaaat accagcttaa ttgagcaaat aatcgcacgc gttgtgtcct gacctgattc
    12181 tggatattta ttcgatgggt aaagaagtgg tgtatttaat ctgttccatc gcgaagctgg
    12241 aagtgacgtc cgacagcccc ggcacgctgt ttaccagacg cttataaaac tcgtcgtagc
    12301 gtttcatgtc ggcaacctgg acgcgcatca gataatcgta ttcaccagcc atgcgccaga
    12361 accccagcac ttctggcatt tcggtaacca ccgtgacaaa gcggcaatac cattcgctgc
    12421 tgtgatgttg cgtttttatc agcacaaagg cggtcaggcc gaggcctatt ttttccggat
    12481 ccagcagggc gactttgccg ataaggatac cgtcgtcctc cagccgtttc aggcgcttcc
    12541 agcaaggggt ggttgtcaga ttaacggctt cagccagtgc ctgcaaagag agggtgcaat
    12601 cctgctgcag taaggccagc agcttacggt caattttatc taacatagcc cttccacaga
    12661 gaattttttt ctcttttaat tctattttaa aggtgaaatc gcgacaattt attctgtgcg
    12721 gtttaaacac cacggcacaa aatgacaaat tcaacgcaaa aaaaagccgg gcaattgccc
    12781 ggtgctggaa ggaatctcgt tattcggggg aataaggtag atgtggatag tccagccagt
    12841 gcgtcaaata gtgagagaca gcctgatttc ggcaatgtcc aatcaccggt aaatgcggga
    12901 gctccgcgcg cagttgcggc atcgcattgc ccataataaa tccgctaccg acgctgccta
    12961 acatttcgcg atcgttcatc gcatcaccaa aggccatgca atcgcgcaac gataaaccta
    13021 aatgttgggt cagtaccgtc aatgcagcgc ctttattgca gcccaccggc agcacttcaa
    13081 ggcaatccgt ggcggaaaaa cacaaatgtg cacgctcgcc taatgcttcg tatagctgga
    13141 tctgcaaacg tgtaagatcg tcgtgatcgc cacagaagca gatcttggtg acgctgccga
    13201 gtggcatttt tttgacatcg attatttgat aacgaaaacc gctatagaca aatgcctgca
    13261 acaacgcagg gatctctttc ccggtaaacc aaccgtcgtc attgaagata tgcatgctgg
    13321 ctcgggtatc ccattgctga tacagcacca gctccgcgac atccgcaggt aaatcatcac
    13381 gatgtaaaag ttcaccttcc agagaatgca cgcgcgttcc gttgccggta atcaaatacg
    13441 catccagcga tagcgccccg agaatatgct gcatctccag cgcatgacgc cccgtggcaa
    13501 aagtgagggt aatgtcgcgt tcacgcagtc gcgccaaagt agagagggtt ttctcaccta
    13561 aatgatgatc aggcatcaat aaagtgccat ccatatcaaa tgctgccaga cgagccattt
    13621 ctttctccac tctgtgacac ggttaatggt gattgagtat cacctgatat atacggaagt
    13681 aatagtgaat agttaaataa tattgttccg ggtttatatg cgactgctca accgtcttaa
    13741 ccagtatcaa cgtctgtggc aaccttccgc cggaaagccg caaaccgtca ccgtcagcga
    13801 actggccgaa cgctgttttt gcagcgaacg ccatgttcgt acgctgttgc gtcaggcaca
    13861 ggaggcggga tggctggagt ggcaggcgca gtcaggacgc ggaaagcgcg gacaattacg
    13921 ctttctggtc acgccagaat cgctacgcaa tgcgatgatg gaaaaggcac tggaaaccgg
    13981 aaagcagcaa gatgtgctgg agctggcgca actggcccca ggtgagctgc gcactctgtt
    14041 acagccgttt atgggcggac aatggcaaaa cgatacgccc acgttgcgta ttccctacta
    14101 tcgcccgctc gaaccgctac aaccgggctt tttgcccggc cgtgccgagc agcatctcgc
    14161 cggacagata ttttccggcc tgacccgctt cgataataat acccagcgcc cgattggcga
    14221 tttagcgcat cactgggaaa cctctgctga cgggttacgc tgggactttt atcttcgttc
    14281 aaccctacac tggcataacg gcgatgcagt aaaagcctca cacttacacc agcgattatt
    14341 gatgctgtta caactgccag cactggatca attatttatt agcgtgaagc gtattgaggt
    14401 cacccatccg cagtgtctga ccttcttttt acatcgccct gattactggc ttgcgcaccg
    14461 gctggcgagc tattgcagcc atctggcgca tccgcaattc ccactgatcg gcacgggtcc
    14521 ttttcgctta acacaattca cagcggagct ggtgcgcctg gaaagccatg attattacca
    14581 tttacgtcat ccgctgctta aagcggttga gtactggata actccgccgc ttttcgaaaa
    14641 agatttggga accagttgtc ggcatcccgt gcaaatcacc atcggcaaac cggaggagct
    14701 gcaacgggtc agccaggtca gtagtggcat cagtttaggt ttttgctatt taacgttgcg
    14761 caaaagtccc cgactctccc tctggcaggc gcgaaaagta atctccatta ttcatcaatc
    14821 cggtttatta caaacgttag aagtcggaga aaacctgatc accgccagtc atgcattact
    14881 gccaggctgg actattccgc attggcaagt accggatgaa gtcaaactac cgaaaacctt
    14941 gacgctggtt tatcacctac cgatagaact tcataccatg gcagaacgcc tacaggcgac
    15001 actggcagcg gaaggctgtg aactcacaat tatttttcat aacgcaaaaa actgggacga
    15061 cacgacccta ctggcacacg cagacctcat gatgggcgac agattaattg gcgaagcacc
    15121 ggaatatact ctggagcaat ggctacgttg cgatccactg tggccacatg ttttcgacgc
    15181 tccagcatat gcccatttgc aatcgacact ggacgcggtg caagtaatgc ctgatgagga
    15241 aaaccgattt aatgccctga aagcggtttt tagccagtta atggcagacg cgacgctgac
    15301 gccgctattc aactatcact atcgcattag tgcccctccc ggcgtgaacg gtgtgcgact
    15361 gacaccgcgc ggctggtttg aatttaccga agcctggctt cccgcgccgt cgcaatgaat
    15421 agctggtccg ggttaatcgc aggcgttacc ataacgcttt tattgttcaa gcaggattat
    15481 ctatgaaacg tgctgtcgtt gtgttcagtg gaggtcagga ttccaccacc tgtctggtgc
    15541 aggcattaca acaatatgat gaagtccatt gcgtgacgtt cgattacggt cagcgccatc
    15601 gcgcagaaat cgacgtggca cgcgaactgg cgctgaaact gggggcacgc gcgcataagg
    15661 ttcttgatgt aacgctgctc aacgagctgg cggtcagcag cctgacgcgt gacagcattc
    15721 cggtgcctga ttatgaacct gaagccgatg gcatcccgaa tacgtttgtc ccagggcgta
    15781 atattttgtt cctgacactg gcggcaatat atgcgtatca ggtaaaagca gaagccgtaa
    15841 ttactggcgt ctgcgaaacg gatttctccg gctacccgga ttgccgcgat gagtttgtga
    15901 aagcactaaa ccatgccgtc agtttgggca tggcgaaaga tattcgtttt gaaacgccgc
    15961 tgatgtggat tgataaagcg gaaacctggg cgctggcaga ttattacggc aaactggatt
    16021 tagtccgtaa cgaaacgttg acctgctata acggcattaa aggcgacggt tgcggtcatt
    16081 gtgcggcgtg taatttacgc gccaacggtt tgaaccatta tctgaccgat aaaccgacgg
    16141 tgatggcagc gatgaagcag aaaacagggt tgaagtaatt attccaagta ttgccggatg
    16201 cggcttgagc atccggcaca acaaaacgtt tacttaacca tctgctccag cttttcgcgc
    16261 aattcccctt ccagagctaa tgctttctgc gttttaagat caatacaaac aaacgtaata
    16321 agcgcatccg ctactacctg cccttccggc tccagtgtaa tgacctggct taagatgccg
    16381 cttttaccgt ttaattgctg taactggctc gtaatggtta acaagtcgct taataccgcc
    16441 gggcgacgat agttaatatt gatattcacc accacgaaag cgatgttatg ggccgtcatc
    16501 cagtgaaaac tgtcgctatt ttccaaccca tcccagcggg cttcttcgag aaattcaagg
    16561 tagcgggcgt tgtttacgtg ctggtaaacg tcgagatgat atccacgaac tttgatttgt
    16621 gtttgcatag cgcaataacc ttacgttgtt tttataaaaa ctgttataag tacagaggtc
    16681 ataactggta tgacctcttc agtctggcaa aaaattgcca ctatgcaaat taattacagg
    16741 gttaataccg ccagattacg ttccaccagc gaattgccca tccccggcac ctgctttaga
    16801 tcctccaccg ttttaaacgg accgtactct tcgcgataac tgacaatcgc ctgcgctttc
    16861 ttcaggccaa cgccattcat cgcgcgggct agctcttccg cgctggcatt attaatgctg
    16921 acccgggtgc cttcttcgtc actggctttc gccggtactg ctgctttact ttgtgctgca
    16981 ggagcttccg ctttggtttc taccgccgtc ggtttcgcca cagaagctgc cgccagcgcg
    17041 ctatgagaca ttccggcaca ggccagggac agggtaatga gcagtgcttt aattccgtgt
    17101 ttcatactgt tttctccttg tttgctaaca gtggaatcac cttaacggga gtgaaaacag
    17161 taaacaaatg gcaatgttca gaaatggaaa aggccgcgaa agcgaccttt tcttgttaca
    17221 gctgcaaagc atttttttgc gaggcggctt caggattatt gctgttccag cgcatcgcca
    17281 attttgattt tcgcctcttt acgcaggtta ctcatcagag cttcaaagac gatttgtgcg
    17341 ttgttctggg tgataccctg caccatcgct tttttctgat cttccggcat tgaaccttgt
    17401 ttcacttcat ccagcgccag cagaaccaca ttaccttgca tatcggtcgc cataccgtag
    17461 ctcggtttgt ctttcgctgg cagtggcagt gcaaacgccg cctggctaat cgggtcacga
    17521 ccggaacggc ttaaggtttt cggctctcca aatttcagac cggcagcctg catagcttcc
    17581 gcacctttgc cggctttcaa atcaaccagc agtttctcag catccacttt cgcctgttgt
    17641 tcagctttgt tgtgctgaac cagtgcctta acttgttcct gaacatctgc caacggtttc
    17701 accgcttccg gtttgtgctc gctgatgcgc agcacgaatg cgcggtcgcc gtctacggtg
    17761 atgatgtcag agttgatgcc cggcgcgccg ttttcaccta ccagaccgcc gttaaagata
    17821 gcgtcggcaa ccggcttgaa gttcaactct tccggcaggt tatctttgct gaaccaaccc
    17881 gtctgagtgg ctttaacgcc ggcagcttgc tctgcaccgg ccagagactc ggtgtcgttg
    17941 cttgccgcat cgctcacttt ctgctgcagc gcgtagtacg catcgagggc tttttcgtgt
    18001 ttcactttcg ccgcaatgtc gtcacgtact ttgtctaacg atttcacttt cgctggctga
    18061 atgtcgtcca gacgtacaat caggaaaccg accgaagatt tgatgacacc agacagttgg
    18121 cctttttctt tcagaccagc atttttcagt tcatccggga tagtggcatc ttctaaccaa
    18181 cccatatcgc cgccgttacg agcagagata atatcggcag atttttcttt ggctaatgca
    18241 gcaaaatcac cgcctttatt cagctcatca agtaccgctt tcgcttcatc ttcagttttg
    18301 gtctggatga tgctgtagcg ggtacgctgc ggctgggtga attgatcctg atgctggtcg
    18361 tagtagctct ggatatccgc atcgctaacc ggttgctgca tcgttgcggc atccagcttg
    18421 atgtaactca cgcggaattg ttccggcgtc atgaaattgt ttttgttttg ttcgtagtag
    18481 ctggcaatct cctgttcggt cacaggctgc ttcgccgcca gcgcgttaac atcgatagtc
    18541 gcttcacgta ccacgcgttg ttgagcgact agtgccgcca gctcgtcggt ttcacctttc
    18601 agcataaaat cggtaccggc aacgccgtta atcagctgtt gggtagtgag ctggttacgc
    18661 agcgcctggg cgtactgatc ggcggtcatc cccatctggt tgaggatacc gttatagcgg
    18721 ctgttatcaa atttgccgtc aacctggaag gctggggtcg cgaaaatcgc ctgtttaacc
    18781 tgctcatcgc tgatacccag tttcagctca cgtgcgtact gatccagcag cgcctcgtcg
    18841 atcagacgat tcagcacctg ttgacgcagg gttttcatat agccttcgtt cgctgccagc
    18901 tcggagtatt gatcgcccag ctgttgctgc atgcgattac gctcgctgtt gaaggcattc
    18961 tcgaactgcc cacggctgat ttcctggtca ttcacttttg cggcgtagtt attgcctccg
    19021 ccaatcaggt aaccactcac gccggtcaat atgaacgaca cgataatgat accgaaaata
    19081 atcttgagca cgagactgtt tgcagccgtg cgtaagctgt ccatcatggt gtaacaacac
    19141 tccgctgtag atgactgtgt acctcacgca acgtatcgat gcgcgcaaag gcctattgtg
    19201 acaagaaacg ggggcaattg tcagcccaca agacacagaa agtcaccgaa tacaaataaa
    19261 aaaggcacat cagtagatgc gcccttgaac ttcgtcacat ccccactggg gacaacgctt
    19321 agtttaccgc gtctttcagt gctttacctg cacggaagct cggtacttta gcggcagcga
    19381 tggtgatctc tttaccggtc tgcgggttgc ggccagtacg ggcagcacgc tctttaacgg
    19441 caaaagtacc aaaacctacc agtgctacat catccccttc tttcagagat tcagttacgg
    19501 aagcaataat agcatctaac gcacggccag ccgcagcttt agagatatca gcccctgcag
    19561 caatcttgtc gatcaattga gatttattca ctcttctctt cctctttata atttatatcg
    19621 cacttgaatc cttcaaggta cgaacgcgca gcagttatat caggccagcc atccccttac
    19681 aacacccctt aataagggca agcccgatcc gccatctaac ttagcgagac aaaaaaaggc
    19741 tggcaagccc gaattagcct gccagccctg tttttattag tgcattttgc gcgaggtcac
    19801 tattttgcag tcacaacctg catgccagac ggttcatttt gcagcgccag agtcagaact
    19861 tcctcaatgc gcttcacagg atgaatgtcc agatcggcaa ttacgttgtc aggaatctct
    19921 tccagatcgc gtttattttc gaacggaatt aacactgttt taatcccacc gcgatgcgct
    19981 gccagtagtt tttctttcaa accaccgatc ggcagtacct gaccacgcag agtgatctca
    20041 ccggtcattg ccacatcggc acgaaccggg ttaccggtca ggcaagaaac cagcgcggtg
    20101 cacatagcaa taccagcact cggaccatct ttcggcgtcg caccttccgg tacgtggacg
    20161 tggatgtcgc gtttttcgta aaaatcaggg ttgatcccca gtttttccgc acgcgcacga
    20221 accaccgtta acgccgcctg aatggactcc tgcatcactt cgccgagcga accggtatag
    20281 gtcagtttgc ctttccccgg aacgcacgcg gtttcaatgg tcagcaagtc accgcccact
    20341 tccgtccacg ccagaccggt tacctggccg acacggtttt cgttatcagc gcgtccatag
    20401 tcgaaacgct gaacaccgag atagtcatgc aagttatcgc cgttaatttc gatatgtttt
    20461 aatgacttat cgagcagtaa ctgcttaacc gctttacggc acagtttgga gatttcacgc
    20521 tccagaccac gcacgcccgc ctcacgggtg tagtaacgaa taatgccgat aatggcgcta
    20581 tcgtcgacgg tcagctcacc ttttttcagt gcattacgtt caatctgctt cggcagcagg
    20641 tgacgtttgg cgatgttcag tttttcatct tcggtataac cggagaggcg aatcacttcc
    20701 atacgatcca gcagcggtgc cggaatgttc atggagttcg acgtcgcgac aaacatcacg
    20761 tcgctgagat cgtaatccac ttccaggtag tggtcgctga acgctacgtt ctgctctgga
    20821 tccagcactt caagcagtgc agaggccgga tcgcctcgca tgtcagaaga cattttgtcg
    20881 atctcatcga gcaggaacag cgggtttttc acgcccactt tcgccatttt ctggatcagt
    20941 ttacccggca tagaaccgat gtaagtacgg cggtgaccac ggatttccgc ttcatcacgc
    21001 acgccgccca gcgccatacg gacatattta cgcccggtgg ctttggcgat ggactgaccc
    21061 agggaggttt tacctactcc cggcggccct accaggcaga ggatcggtcc cttgattttg
    21121 ttgacacggc tttgaaccgc aagatactca aggattcgat ctttcacgcg ctccagacca
    21181 taatggtcgg tatcaaggat ttcctgcgcc tgacgcaggt cttttttgac cttgctgcgc
    21241 gcattccacg gtacctgtac catccagtcg atataaccac gcactacggt cgcttctgcc
    21301 gacatcggag acatcatttt cagcttctgc aactctgctt ccgctttctc ttttgcctct
    21361 ttcggcatct tcgccgcgtc gattttgcgc ttcagggctt cgttttcgtc cggcgcgtcg
    21421 tccatttcac cgagttcttt ctgaatagct ttcatttgct cgttcagata gtactcacgc
    21481 tgggatttct ccatctgctt tttaacgcgg ttgcgaatgc gtttctcaac ctgcagcaga
    21541 tcgatttccg attccatcat tgccatcaga tattccagac gttcgttaac gtcggacatc
    21601 tccagaacgg actgtttgtc agccagtttc agcggcatat gtgcagcaat ggtatccgcc
    21661 agacgcgccg gatcgtcgat gctattcagc gacgtcagca cttctggtgg gatttttttg
    21721 ttcagcttga tgtagccttc gaactggctg attgcagtac gcaccagcac ttcctgttct
    21781 cgctcatcaa tggtcggcga ctccagatac tccgccttcg cagaaaagtg ttcgccattg
    21841 tcagagagcg cagaaatacg cgcgcgctgt aacccctcga ccagcacttt gacggtgccg
    21901 tcaggcagtt tcagcatctg caatatagag gccacggtcc cgacggtgaa aagatcgttt
    21961 acacccggct catccgttga agcttctttc tgcgcgacca gcataatttt tttatcatgg
    22021 tccatcgccg cttccagaca acggatagat ttttcccgcc cgacaaataa ggggatgacc
    22081 atgtgcggat aaaccaccac atcgcgcagc ggcaatacgg ggatttcaat gcgttcagaa
    22141 cgctcaggat tcatagagct ctctcttagt ttaatttccg ccaggtaatc agatgacacg
    22201 actgtgcttc acgccatcta ttaacatgta cgtcagtata tggggatgtt tcccccacat
    22261 tcaacgccga gaatagagga aaaattaaag gggagataaa atcccccctt tttggttaac
    22321 taattgtatg ggaatggtta attattcacc agatgcctgt tgcgcttccg gcttgccata
    22381 aatcagcaac ggtttgcttt gaccatcaat taccgactcg tcgataacca ctttttcgac
    22441 atcttccatg gacggcagat cgtacatggt atcgagcagt gcggcttcta cgatggaacg
    22501 caggccacgg gcaccggttt tacgcgccat cgctttctta gcgatagcat ccagcgcctc
    22561 gtcacggaat tccagatcca cgccttccag attaaacagc gcctgatact gcttggtcag
    22621 ggcgtttttc ggctctttga ggatctgaat cagagcttct tcgctcagtt cattcaacgt
    22681 tgcgacaacc ggcagacgac caataaactc agggataaga ccaaacttga tcagatcttc
    22741 cggttcaacc tgcgccagca gctcgccttc gcttgctttg tcggacttcg cttttaccgt
    22801 cgcgccaaaa ccaatgccgg agccggtttc tacacgatgg gaaatcactt tatccagacc
    22861 ggcaaacgca ccgccacaaa taaacaggat cttagaggta tcaacctgca agaattcctg
    22921 ctgcggatgt ttacgcccac cttgcggtgg aacagcagct accgtacctt cgatcagttt
    22981 caacagtgcc tgctgtacgc cttcaccgga aacgtcacgg gtaatagacg ggttgtctga
    23041 cttacgagaa atcttgtcga tttcatcgat gtagacaatc ccgcgctgcg ctttctgtac
    23101 gtcgtaatcg cacttttgca acagcttctg aatgatgttt tcaacgtctt cgcccacata
    23161 accggcttcg gtcagcgtgg ttgcgtcggc catggtgaac ggaacgtcca gcaggcgcgc
    23221 cagcgtttca gccagcagcg ttttaccgga accggtcgga ccgatcagca gaatgttact
    23281 tttgcccaac tcgacgccat tgctggtatc gccgttgcgc agacgtttgt agtggttgta
    23341 taccgcgacc gccagcactt ttttcgcctg ttcctggccg ataacgtaat cgtccaggtg
    23401 gttacgaatt tcatgcggcg tcggtagcgc actgcgttca cgatgcggtg caacttcttt
    23461 aatctcttcg cgaatgatgt cgttacataa atcaacacat tcgtcgcaga tatacacgga
    23521 tggaccggca atcagcttgc gcacttcatg ctggcttttg ccgcaaaaag agcaatacag
    23581 caatttgcct gagccatctt tgcgtttatc tgtcatgggt caaaacctct tctttgttct
    23641 ttgtgccgca cacgacgacg caaatgccat tctcaggcgc aagccgctta cagcgttgtg
    23701 ccgccctgga taagtatagc ggcacagttg cgcctctggc atcaattacg atgggtcaga
    23761 atcgaatcga ccagaccgta ttccaccgct tcaggggcgg aaaggaagcg atcgcgctcg
    23821 gtatcacgtt caatctgttc taatgattta cccgtatgaa gcgccataag ttcattcata
    23881 cgccctttaa ctttcagaat ttcacgggca tgaatttcga tatcggtcgc ctggccctgg
    23941 tagccgccca acggttggtg aatcatcacg cgcgaattcg gcaggcaaaa acgtttacct
    24001 tttgcccctg cggtcagcaa gaaagcgccc atcgaggccg cctggcccat acagatggtg
    24061 ctgacatcag gcttgataaa ctgcatggtg tcatagatag acatcccggc agtgatcacc
    24121 ccgcctgggg agttaatgta cagatagata tctttttctg ggttttctgc ttccaggaac
    24181 agcatctgcg ccacaatcag gttagccatg tggtcttcaa cctggccagt cagaaaaatg
    24241 acgcgttcct taagtagacg agaatagata tcaaaagagc gctcaccacg tgaggtctgt
    24301 tcaatgacca tcggcaccag cgccatatgg ggtgcaaagt tatctcgttc gccgctgtat
    24361 gacatttccg tctcctggat aaaattgaaa aaacctgctg taccgattgt aaccttatgg
    24421 acggattatc agctagtccc tgtgttatgg gtacggcatc accatatttc aagcataaca
    24481 atcttttgtt gttacgctaa caccgaaaga ggcttttcgc acggttccat gcaaaattca
    24541 tgacaaaaaa aagcccacca cctggcggtg acgggccttt gtgcgaattt agcgcgttat
    24601 gctgcgtaaa ttacgcctgc tggttcatca gctcgttgaa agtggtttct ttttcagtca
    24661 ctttcgcttt cgccagtaca gcttcaacag cctgttcttc cagagcaaca ttgcgcatgt
    24721 tgtccatcag ttctttgttt ttgctgtaga actcgataac ttctttcgga tcttcgtacg
    24781 cagaagccat ctcttcgatc aggcctttca cgcgctcttc gtcagctttc agctcgttgg
    24841 tgcggataac ttcgcccagc agcaggccaa caactacgcg gcgtttagcc tgttcttcga
    24901 acagttcgcg cggcagttcc agagcttgtt tttcgttgcc accgaaacgc tgtgcagcct
    24961 ggcgacgcag aacgtcgatt tcgctgtcaa tcagcgcagc cggtacgtcg atgtcgttag
    25021 cttttaccag accttcgatc gcctgagact taacgcggtt acggatggcg ctcttcagct
    25081 cgcgctccat gtttttacgc acttcagcgc gcagaccttc tacggaacca tcttcaacgc
    25141 cgaaacgttt gatgaattct gcagtcagtt ccggcagttc acgctcttca actttcttca
    25201 ggttgatagc gaatttcgct gctttacctt tcaggttttc tgcgtggtat tcttccggga
    25261 aggtcacgtc gatggagaac tcttcgccag ctttgtggcc tttgataccg tcttcaaagc
    25321 ccgggatcat acgaccctgg cccatcgcca gtacgaaatc agacgcttta ccgccttcga
    25381 actcttcgcc gtctacagaa ccggtgaagt cgatggttac gcggtcttct gcttcaacag
    25441 cgccgtcttt ttctttccag gtcgcctgct gtttacgcag agtatccagc atgccgtcaa
    25501 cgtcagcgtc ggtcacttca acgatcggtt tttcaacttc gatcgcttcc agaccctgca
    25561 gttcaacttc cggataaact tcaaactcta cagagtaggt gaagtcttca cccagcttgt
    25621 attcgcccgg aacataagtc ggtgcgccag ccggattgat tttttcttta atgatggcgt
    25681 caatgaagtt acggctcatc aggtcaccca gaacgtcctg gcgtacagac gcgccataac
    25741 gctgagcaac gatattcatt ggcactttgc ctttgcggaa gccgtcaata cgtacttttt
    25801 ttgcaacgtt gaccagctcg cttttaacag cggtctcgat gctgtcagca gcgatagtaa
    25861 tcgttacacg gcggccaagg ccttgagtgg tttcaactga aacttgcatc ttgttacctc
    25921 aaaaaatcac agtgctcggt caactcttac cccgcaagca caggttgttg gcttcgcgga
    25981 accgggatgt tcttataatc aatcacattc cctgttacca gaatcatccc gaaagcattc
    26041 aaataggacg cggcattata gcggcatcac ttttatgagt cgagaacggt tattgcgcgc
    26101 tgtcgcgtat tttttcacaa ttcccgggca attttcgtct gaatgctggg caaaaagcgc
    26161 aaaatccaga caaaacaaaa cggcccgcag gccgtttttc ataaaatctt tagcaacata
    26221 ctggaaaagc gccgacagtt gcaaatgcgt ttttacgcga tgcttcctgc tccacgacag
    26281 ggaggagagg caaagacggt gtcctgcaac ccttcccact ccttaatagt gtaagtatgc
    26341 agagccagcg catgaacggt agtagagagt tcctccgcta aagtactgta aatcattcga
    26401 tgacgattca gaaaacgttc acccgtaaaa cgatcgctga ccagcacaac tttaaaatgg
    26461 ctttcagagc cggctgggac attgtgacga tagctttcat ccactacttc gaggaatacg
    26521 ggttggaacg ccgcccttaa tttttcttct atccgctcac gtatcatcat gaaatatctc
    26581 ctccgacaac gctgagatgt catccatccc tttaaatact agccgctttt accgtttcca
    26641 ttgcagctta acaacaaata tttaggtttc gtcctggttt ttccgtcaag cgtatgaagt
    26701 ttactgattt aaagattgaa acacaggcct cgcatcagcg tttcttttac caggggcgat
    26761 tatttcgtca acatgatgaa tttacatgcc ttacctactt cccctcgccg ttggcaatgt
    26821 tatgatggcg ggaattttta ccaaacacgc ttaagagtct ctgagagccc gaacatgttc
    26881 aaaaaaatcc tcttcccgtt agttgctctg tttatgcttg caggatgcgc aaaaccgcca
    26941 acaactattg aagtttcccc gacgattaca ctgccacagc aggatccaag cctgatgggc
    27001 gtcaccgtaa gcattaatgg tgccgatcag cgtaccgatc aggcgctggc aaaagtcacc
    27061 cgcgataatc aaatcgttac cctgaccgcc tcccgcgatc tgcgtttcct gctgcaagaa
    27121 gtgctggaaa aacagatgac cgcgcgtggt tacatggttg gtccgaatgg cccggttaat
    27181 ctgcaaatca tcgttagcca actgtatgct gacgtgtccc agggcaacgt gcgctacaac
    27241 atcgctacca aagcggatat cgccatcatc gctaccgcgc agaatggcaa caaaatgacc
    27301 aaaaactatc gtgccagcta caacgttgaa ggtgcgttcc aggcctccaa caaaaatatc
    27361 gccgatgcgg ttaacagtgt gctgaccgat accatcgctg atatgtctca ggacaccagc
    27421 attcacgaat tcatcaagca gaacgcgcgt taatttctgc cctctggccc ggtgcaagcc
    27481 gggcctgtag acgcccatgt ccagtcaata tttacgtatt tttcaacagc cgcgttcagc
    27541 catattgctg atcctgggtt ttgcttccgg gctaccgctc gccctgacat ccggcacctt
    27601 acaggcctgg atgacggtcg agaatatcga tctcaaaacc attggtttct tctctctggt
    27661 aggccaggct tacgtcttta aattcctctg gtcaccgctg atggaccgct acacgccccc
    27721 attttttggg cggcggcgcg gttggctgct cgccacgcaa atcctgttat tagtcgccat
    27781 tgcggcgatg ggttttctcg aaccaggcac ccaactccgc tggatggcgg cgctggcggt
    27841 ggtgatcgct ttttgctctg cctcccagga tattgtcttc gatgcgtgga aaaccgatgt
    27901 gctcccggca gaagaacgtg gtgcgggcgc ggcaatcagc gtgctgggtt accgtttagg
    27961 gatgttggtt tccggcggcc tagccctgtg gctggcagat aaatggctgg gctggcaggg
    28021 catgtactgg ttgatggcgg cgctgttgat cccctgtatt atcgcgacgt tgcttgcacc
    28081 agaaccaacc gacaccattc ctgtgcccaa aacgctggaa caagcggttg ttgcacctct
    28141 gcgagatttc tttggtcgca ataatgcctg gcttattttg cttcttatcg tgctgtataa
    28201 gctgggcgac gcattcgcca tgagcctgac aaccacgttt ttgattcgcg gcgtcgggtt
    28261 tgatgcgggt gaagtaggcg tggttaacaa aacactgggc ttactggcga ccattgttgg
    28321 cgcattgtac ggtgggattt tgatgcagcg cctgtcactg ttccgggcac tgctgatttt
    28381 cggcatttta caaggtgcgt ctaacgctgg ttactggctg ctgtcgatta ctgataagca
    28441 tctctacagc atgggcgcag ccgtcttttt cgaaaacctc tgtggcggga tgggcacatc
    28501 agcctttgtc gcgctgttaa tgacgctatg taataagtca ttttccgcta ctcaatttgc
    28561 cctgctctca gcgctttctg ctgtagggcg agtttatgtc ggccccgtgg cgggttggtt
    28621 tgttgaagca cacggctggt cgacattcta tctattctcc gtcgccgctg ccgtaccagg
    28681 gcttattttg ctgctggttt gccgccagac gcttgaatat acacgagtaa atgacaactt
    28741 tatctcccgt accgcatatc cggcaggtta tgcctttgcc atgtggacac tggcggcggg
    28801 cgtcagcctg ttggccgtgt ggttactgct gttgacgatg gacgcgctgg atttgacgca
    28861 cttctctttc ctgcctgctc tgctggaagt cggggtttta gtcgcccttt ctggcgtcgt
    28921 gcttggtggt ttgctggatt atctggcgct acgaaaaacg catct
//
LOCUS       DAZKFU020000033        28747 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZKFU020000033 DAZKFU020000000
VERSION     DAZKFU020000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.033"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..513
                     /locus_tag="ACHOQQ_RS05850"
                     /old_locus_tag="pgaptmp_001170"
     CDS             <1..513
                     /locus_tag="ACHOQQ_RS05850"
                     /old_locus_tag="pgaptmp_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4469446.1"
                     /translation="KFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETK
                     TVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGG
                     AVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRI
                     QPNTKCPEKS"
     gene            529..1068
                     /locus_tag="ACHOQQ_RS05855"
                     /old_locus_tag="pgaptmp_001171"
     CDS             529..1068
                     /locus_tag="ACHOQQ_RS05855"
                     /old_locus_tag="pgaptmp_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGU4469447.1"
                     /translation="MKKVFLCAILVSLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1161..2738)
                     /gene="guaA"
                     /locus_tag="ACHOQQ_RS05860"
                     /old_locus_tag="pgaptmp_001172"
     CDS             complement(1161..2738)
                     /gene="guaA"
                     /locus_tag="ACHOQQ_RS05860"
                     /old_locus_tag="pgaptmp_001172"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGU4469448.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFITVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2807..4273)
                     /gene="guaB"
                     /locus_tag="ACHOQQ_RS05865"
                     /old_locus_tag="pgaptmp_001173"
     CDS             complement(2807..4273)
                     /gene="guaB"
                     /locus_tag="ACHOQQ_RS05865"
                     /old_locus_tag="pgaptmp_001173"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGU4469449.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4435..5805
                     /gene="xseA"
                     /locus_tag="ACHOQQ_RS05870"
                     /old_locus_tag="pgaptmp_001174"
     CDS             4435..5805
                     /gene="xseA"
                     /locus_tag="ACHOQQ_RS05870"
                     /old_locus_tag="pgaptmp_001174"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGU4469450.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5802..6017)
                     /locus_tag="ACHOQQ_RS05875"
                     /old_locus_tag="pgaptmp_001175"
     CDS             complement(5802..6017)
                     /locus_tag="ACHOQQ_RS05875"
                     /old_locus_tag="pgaptmp_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4469451.1"
                     /translation="MELHCPQCQHVLDQDNGHAHCPSCGEVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6086..7558)
                     /gene="der"
                     /locus_tag="ACHOQQ_RS05880"
                     /old_locus_tag="pgaptmp_001176"
     CDS             complement(6086..7558)
                     /gene="der"
                     /locus_tag="ACHOQQ_RS05880"
                     /old_locus_tag="pgaptmp_001176"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGU4469452.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7676..8854)
                     /gene="bamB"
                     /locus_tag="ACHOQQ_RS05885"
                     /old_locus_tag="pgaptmp_001177"
     CDS             complement(7676..8854)
                     /gene="bamB"
                     /locus_tag="ACHOQQ_RS05885"
                     /old_locus_tag="pgaptmp_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGU4469453.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8865..9485)
                     /gene="yfgM"
                     /locus_tag="ACHOQQ_RS05890"
                     /old_locus_tag="pgaptmp_001178"
     CDS             complement(8865..9485)
                     /gene="yfgM"
                     /locus_tag="ACHOQQ_RS05890"
                     /old_locus_tag="pgaptmp_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGU4469454.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9503..10777)
                     /gene="hisS"
                     /locus_tag="ACHOQQ_RS05895"
                     /old_locus_tag="pgaptmp_001179"
     CDS             complement(9503..10777)
                     /gene="hisS"
                     /locus_tag="ACHOQQ_RS05895"
                     /old_locus_tag="pgaptmp_001179"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGU4469455.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10888..12006)
                     /gene="ispG"
                     /locus_tag="ACHOQQ_RS05900"
                     /old_locus_tag="pgaptmp_001180"
                     /gene_synonym="gcpE"
     CDS             complement(10888..12006)
                     /gene="ispG"
                     /locus_tag="ACHOQQ_RS05900"
                     /old_locus_tag="pgaptmp_001180"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGU4469456.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12033..13046)
                     /gene="rodZ"
                     /locus_tag="ACHOQQ_RS05905"
                     /old_locus_tag="pgaptmp_001181"
     CDS             complement(12033..13046)
                     /gene="rodZ"
                     /locus_tag="ACHOQQ_RS05905"
                     /old_locus_tag="pgaptmp_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGU4469457.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13331..14485)
                     /locus_tag="ACHOQQ_RS05910"
                     /old_locus_tag="pgaptmp_001182"
     CDS             complement(13331..14485)
                     /locus_tag="ACHOQQ_RS05910"
                     /old_locus_tag="pgaptmp_001182"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGU4469458.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14635..15066)
                     /gene="ndk"
                     /locus_tag="ACHOQQ_RS05915"
                     /old_locus_tag="pgaptmp_001183"
     CDS             complement(14635..15066)
                     /gene="ndk"
                     /locus_tag="ACHOQQ_RS05915"
                     /old_locus_tag="pgaptmp_001183"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGU4469459.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15206..16060)
                     /locus_tag="ACHOQQ_RS05920"
                     /old_locus_tag="pgaptmp_001184"
     CDS             complement(15206..16060)
                     /locus_tag="ACHOQQ_RS05920"
                     /old_locus_tag="pgaptmp_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4469460.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIECAVCQFICPQEAIRGVNSPLRYFRNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNERLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRAP"
     gene            complement(16060..16881)
                     /locus_tag="ACHOQQ_RS05925"
                     /old_locus_tag="pgaptmp_001185"
     CDS             complement(16060..16881)
                     /locus_tag="ACHOQQ_RS05925"
                     /old_locus_tag="pgaptmp_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HGU4469461.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSAPPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16874..17503)
                     /locus_tag="ACHOQQ_RS05930"
                     /old_locus_tag="pgaptmp_001186"
     CDS             complement(16874..17503)
                     /locus_tag="ACHOQQ_RS05930"
                     /old_locus_tag="pgaptmp_001186"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HGU4469462.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17500..19881)
                     /locus_tag="ACHOQQ_RS05935"
                     /old_locus_tag="pgaptmp_001187"
     CDS             complement(17500..19881)
                     /locus_tag="ACHOQQ_RS05935"
                     /old_locus_tag="pgaptmp_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HGU4469463.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSM
                     DTLLDTKLVILWGHNPTETIFGHTNYFFQKMKQNGTRFIVVDPRYSDTVSSLADQWIP
                     LLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAK
                     DGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRHTCGERTARGSTLL
                     ASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQ
                     DGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSA
                     KYADVLLPETSFMERWNIGETWGTASYLILSEKLIEPDFERRTDYDWLRDVAKKLGVE
                     AEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDP
                     QNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGK
                     NRANSTQYANPWLQEVQTQKLWLNPQDAKQRGIVEGDSVKIYNDRGVSIIPVEITPRI
                     IPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
     gene            complement(20045..22357)
                     /gene="pbpC"
                     /locus_tag="ACHOQQ_RS05940"
                     /old_locus_tag="pgaptmp_001188"
     CDS             complement(20045..22357)
                     /gene="pbpC"
                     /locus_tag="ACHOQQ_RS05940"
                     /old_locus_tag="pgaptmp_001188"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGU4469464.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARQGKAGKLRLQPDDPLLERPLMSSEAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQAQQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
     gene            complement(22358..27319)
                     /gene="yfhM"
                     /locus_tag="ACHOQQ_RS05945"
                     /old_locus_tag="pgaptmp_001189"
     CDS             complement(22358..27319)
                     /gene="yfhM"
                     /locus_tag="ACHOQQ_RS05945"
                     /old_locus_tag="pgaptmp_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HGU4469465.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLEVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRCEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSTEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            27526..28371
                     /gene="sseA"
                     /locus_tag="ACHOQQ_RS05950"
                     /old_locus_tag="pgaptmp_001190"
     CDS             27526..28371
                     /gene="sseA"
                     /locus_tag="ACHOQQ_RS05950"
                     /old_locus_tag="pgaptmp_001190"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGU4469466.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
BASE COUNT         7001 a         7873 c         7057 g         6816 t
ORIGIN      
        1 aaatttaaaa aatgtcttct gcctgtggca atgttagcgt cattcactct ggcaggatgc
       61 cagtcaaatg ctgacgatca tgccgccgat gtttatcaaa ccgatcaact gaataccaaa
      121 caagaaacta aaaccgttaa tattatttcc attcttcccg caaaagttgc cgtagacaac
      181 tcccaaaata aacggaacgc acaagccttc ggcgcgctta ttggcgcagt cgctggcggt
      241 gttatcggcc acaacgtcgg gtctggcagc aattccggaa cgacggcagg tgcagttggc
      301 ggcggagctg taggcgcggc agcgggttct atggtgaatg ataaaacctt agtggaaggt
      361 gtttctttaa cgtataagga aggcaccaaa gtgtatacct ctactcaggt gggtaaagag
      421 tgccagttta cgacaggttt agccgttgtt attaccacga cgtataacga aacgcgtatt
      481 cagccaaata ccaaatgtcc tgaaaagagc taataatcag gaggagtcat gaagaaagtt
      541 tttctttgcg ccatcttggt ctccttaagc tatccggcta tcgcctcatc attgcaggat
      601 caactctctg ctgtcgcaga agcggaacag caaggtaaaa atgaagagca aaggcagcat
      661 gacgaatggg tcgcggagcg caacagggaa atccagcaag agaagcaacg tcgcgcaaat
      721 gcccaggccg ccgctaacaa aagagcggca acggcagcgg caaataagaa agctcgtcag
      781 gataaactgg acgccgaagc cactgcggac aaaaaacgcg atcaaagtta tgaagatgag
      841 ctacgcagct tagagattca gaaacaaaaa ctggcgctgg cgaaagaaga agcccgcgtt
      901 aagcgcgaaa acgaatttat cgatcaggaa ctgaagcaca aagctgcgca aaccgatgtg
      961 gtgcaatctg aagctgacgc caacagaaat atgactgaag gcggtcgcga tctgatgaaa
     1021 agcgtgggca aagcagaaga gaacaaatcg gacagctggt ttaattaatc gatgttacta
     1081 acttcaagcc tatgattctt gaagataaaa aaccctctgt agtaacagag ggttttgttc
     1141 attcatagtg cagggtcaaa tcattcccac tcaatggtag ctggcggctt gccgctgatg
     1201 tcatacacca cgcgggaaat accgttcact tcattgataa tgcggttgga aacgcgaccg
     1261 aggaaatcgt acggcagatg cgcccagtga gcggtcataa agtcgatggt ttcgaccgca
     1321 cgcagagaaa caacccagtc atacttacga ccatcgccca ttacgccaac ggaacgtact
     1381 ggcaggaaga cggtgaacgc ctggctgact ttgtcgtata ggtccgcttt acgcagttct
     1441 tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt cacttcaccc
     1501 agaacacgaa cgccaaggcc tggtcccggg aacgggtgac ggtacagcat gtcgtacggt
     1561 agacccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
     1621 accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg
     1681 tgtgctttac cggttgcaga cgccgcagat tcgataacgt cagggtagat ggtgccctgc
     1741 gccagccact tcacgtcctc cagtttcagc gcttcttcat cgaatacttc aacgaaaacg
     1801 cgaccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc tgacaggaag
     1861 cgatcttctg ccggtacgtg aacaatgttc agaccaaagt gatcgccaaa catatccaga
     1921 acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca agtcaggttt
     1981 ttaccgatag cgcggtgcag cagcattgcg gttacggagg aatccacacc accagagagg
     2041 ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc gagctacagc atcatcgata
     2101 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
     2161 atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
     2221 ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac ggtgatgaag
     2281 tcggacggga tagcggtaac tttatcgccg tggctcatcc agacatcgag cagcggttta
     2341 ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca
     2401 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
     2461 gccatggtct gcatgccata gcaaacgccg aataccggta cgcctgcttc aaagacatac
     2521 tgcggcgcac gcggactgtt ttcttcagta gtactttccg ggccgccgga aagaataatg
     2581 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca cagttcgcag
     2641 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtcc
     2701 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
     2761 gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag gagcccagac
     2821 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg
     2881 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
     2941 tcagacccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
     3001 ctttataagc tacgcgacct tcgatacctt ccggcaccag tttgtcggca gcgttatcgc
     3061 tctggaaata acggtcagag gaacctttgg acatcgcgcc cagggaaccc ataccacggt
     3121 aagatttgta agaacggccc tggtagagtt cgatttcacc cggagattct tcagtacccg
     3181 ccagcatgga acctaccatc accgcgcttg cgccagcggc gatagctttg gcgatgtcgc
     3241 cggagaagcg aataccacca tctgcgataa ccggaatacc ggtgccttcc agggcttcaa
     3301 ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc agtaacgata cgagtcgtac
     3361 agatagaacc tgggccgata ccgactttaa ccgcgctgca accagcttct gccaaagcgc
     3421 gtgcacctgc agctgttgcc acgttgccgc cgatgatttg cagatccgga tatttagcac
     3481 gggtttcacg gatacgttgc agtacacctt ctgagtgacc gtgggaggag tcgatcagca
     3541 gaacgtcaac gccagcggca accagcgcgt caacacgctc ttcgttaccc gcacctgcgc
     3601 caactgcagc accaacacgc agacggcctt gctcgtcttt acaggcgttc ggtttacgtt
     3661 ccgctttctg gaaatctttc acggtgatca tgccgatcag gtggaattcg tcatcaacca
     3721 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcac
     3781 cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggttca
     3841 ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc caccagttcg ttttcttcgg
     3901 taacgaccgg atagcccgca aaaccgttac gctcggtcag ttctttcact tcgcgcagcg
     3961 tcgtggttgg cagaacagtc tgcggatcag tcaccacgcc agattcgtgt tttttcacac
     4021 ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag ccgataccgc
     4081 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
     4141 gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca gcagtattcg
     4201 gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
     4261 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
     4321 tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct atccccgaag
     4381 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
     4441 ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg
     4501 cttgagcatg agatgggaca ggtttggatc agcggcgaaa tctctaattt cacacaaccg
     4561 gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg
     4621 ttccgcaaca gcaaccgccg ggtgaccttc cgcccacagc atgggcaaca agttttagtt
     4681 cgcgccaata ttacactcta cgagccgcgc ggcgactacc agataatcgt tgagagtatg
     4741 cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc gaagttgcag
     4801 gctgaaggat tgttcgatct gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt
     4861 ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt
     4921 cgcgatcctt ctctgccggt gatcatctac cccaccgccg ttcagggcga tgacgcgccg
     4981 gggcaaattg ttcgcgccat tgaactggcg aatcagcgca atgagtgcga cgtgttgatc
     5041 gttgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga acgcgtagcg
     5101 cgggcgattt ttgccagccg cattccggtc gtcagcgccg tcgggcatga gacggatgtg
     5161 accattgccg attttgttgc cgatctgcgt gcaccaacac cgtcggctgc cgccgaagtg
     5221 gtgagccgta atcagcaaga gttactgcgc caggtgcaat cgacccgtca acggctggag
     5281 atggcgatgg attattatct cgccaaccgc acacgtcgct ttacgcaaat tcatcaccga
     5341 ttacagcaac agcatccgca gctccggctg gcacgccagc aaaccatgct tgaacgcctg
     5401 caaaaacgga tgagctttgc gctggaaaat caacttaagc gtgccgggca acagcagcag
     5461 cgattaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg cgcgcaaacg
     5521 cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgcacagct tagcgccacg
     5581 cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact gtcaacgctg
     5641 gcgcgtggat acagcgttac tactgctact gacggcaatg tactgaaaaa agtgaagcaa
     5701 gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg gctggataga aagtgaagta
     5761 aaaaacatcc agccggtaaa aaaatcgcgt aaaaaggttc attaagccag cacgaattca
     5821 acgcgttttt tcgaaatcaa gccatgcccg tgctgacaga agtaatctac cgcaccgcag
     5881 gcttttaaca cctgcaacgg ttgatggcag tccgggcaaa gagctttcat ttcaataact
     5941 tccccgcagg atgggcaatg cgcatggccg ttatcctgat caagcacatg ctgacattgt
     6001 ggacaatgca gttccatatg aattcctccg gtagccaccc ctctacattc atagagggaa
     6061 tggcagataa aatacttacg gataattatt tatttttctt gatgtgcttc atcagacgct
     6121 tacgtttacg catctgggtt ggcgtcaggg tgttacgctt gttcgcatac gggttttccc
     6181 cttctttgaa ctgaatacga atcggcgatc ccattacgtc cagcgatttg cggaagtagt
     6241 tcatcaagta acgcttgtag gaatcaggca ggtctttcac ctgattaccg tgaatcacca
     6301 caatcggcgg attataacca ccggcgtgtg catatttcag cttcacacga cgaccgcgta
     6361 ccagcggcgg ttggtgatct tcaacagcca tcgtcatgat gcgcgtcagc atagaggtcc
     6421 ccacacgacg ggtggagctg tcatacgctt cacgtactga ttcaaacaag ttaccaacac
     6481 cactgccgtg caaggcagag ataaagtgca cacgagcaaa atcgataaag cccagacgga
     6541 agtccagcgt ttctttcacc tgctctttca cttcctggct caggccatcc cacttattca
     6601 ccacaatgac aagtgagcgc ccactattga gaataaagcc cagcagcgag agatcctgat
     6661 cggaaatacc ttcgcgcgca tcaatcacta acatcaccac gttggcgtct tcaatggcct
     6721 gcaacgtttt gattacggag aatttctcta cagcatcggt gattttgccg cgtttacgta
     6781 cgccagcggt gtcaatgagc acatactcac gtccatcgcg ttccattgga atgtagatac
     6841 tgtcacgcgt cgtaccaggc atgtcataaa caacaacgcg ctcttcacca agaatacggt
     6901 tagtgagtgt agacttacct acgttcggac gacccacaat cgccagtttg atcggcagac
     6961 tttgcgggtc gaagtcgtct tcctcttctt cttcgccgtt ctcttccgct tcaaattgcg
     7021 cccagtattc agcgtcttcg tcgacttcct cttgcggtgc gagatcttcc atccacggca
     7081 gtagcacatg ctccagcaga cttaatacgc cacgaccgtg agacgcggcg atcgggtaga
     7141 tttcacctaa accaagcgag tagaaatcaa ccactgcctg atcgggatcc agaccgtcag
     7201 ttttgtttgc caccaggaag gtcggttttt cacgggagcg cagatgtttg gcaatcgctt
     7261 catctgccgg catcaggccc gcgcgcgcat ccaccataaa cagtacgacg tccgcttctt
     7321 caatcgccag cagcgactgt tccgccatgc gggtttctac accgtcttct gtgccatcaa
     7381 tcccgccggt atcaatacag ataaactcac ggccttcaat ttccgcacga ccgtacttac
     7441 ggtcacgagt cagacccggg aaatccgcaa ccagcgcatc tcgggtgcga gttagacggt
     7501 taaataacgt ggattttcct acgttagggc gcccgacaag cgcgaccaca ggtaccatgt
     7561 ttaaagcctc atttttataa atcatcagac aacgcacgct atattcgcgt cgttgttaaa
     7621 aacaggaaaa cggcccctgt ccaggagccg ttttcaaagt gaacgacaga gacgattaac
     7681 gtgtaataga gtacacggtt ccgtcttttg cctggatcag cagtttgccg tcagcggcaa
     7741 ccggttcagt ctggaaaccg gaactatcaa ctttttgctg ggcaacgaaa cgaccatctt
     7801 cgacgttaat ccagtgcaaa taaccttcac tgtcaccgac caccaggttg ccattataca
     7861 gcaccggaga agtcagcagg cgatgcagca gatcgctttg tgtccacagc gtaacgccgc
     7921 catcaatggt caacgccatc acccggtcat tttgatcgac cagatagatg cgattgccgt
     7981 cgacgatgaa atcattcacc gaacccagtt cgcgtttcca cataatctga ccactgcgca
     8041 gatcaagcgc cgtcaggtta ccattatagg ccagcgcgaa aacaacgccg ttaacaacga
     8101 cgggagtcgt gtcaacatcg ctcagacggt caatttcggt agaaccggtc gcctgggaaa
     8161 tacgctgctg ccaaatcatc tggccctgtt ccatcagcac tgcgctgacg cgaccattat
     8221 cgccccccac gacggccgca ccaaaagccg ttgccggcgc agactcgcca cgcaaagaga
     8281 gcgaaggcat atcgaggtta actgtccatt tgacagcgcc gtcagcttcg ttcagcgctt
     8341 gtaactgacc gttactggtg tggattaaca ccagaccgtc gctgaccacc gggcgcgaaa
     8401 gtgcttcacc cgcgacttta gtttgccatg ccacagtacc atcgctggta ttcagcgcgt
     8461 aaacctgcgc cttttcgctg ccaatgtaga catgcccacc agacacggtc acaccgccag
     8521 aaagtaatgc aggctcttta gagaaccagc catctttctc ggccaggctg acagaccaga
     8581 tttctttgcc atcatccgca ttcagcgctt ttactaaacc agcgcggtcc gctgcataga
     8641 caacgttgtc cgccagtgcc ggatgaagat tggaatagaa gttgccaatg ccgctaccaa
     8701 cggaagtgct ccacgccgtg gtcggcgtaa actggttttc aacggttggc aatggggaca
     8761 tctttaccac atcttcttcg ctgttaaaca gcgaacagcc gcttaaaagg gtaacggaaa
     8821 gcagtcctgg cagcagtaat ttacgcaatt gcatcgggtc cctctcagat ggacaaatta
     8881 ttaattttca tctgcatcat ttcgctcagt gccggagtaa catcgctttt cacgcctgct
     8941 tcccatgcac tacgcgcacc ttgcttatca cctttgctca gcaatgcttc accacgcagg
     9001 tcggcaacaa tggcagccca cccttcacct ttgatggtat caagggtttt cagcgcggca
     9061 tcagcctgct tgagctgtac ctgaacgcga gcaagacgca gatttatcac ggctttgaga
     9121 ttttcatcgc tcgtgtctgc cagcccctgt tgtaactggg cggcagcttt ctctagttca
     9181 tttttgtcaa caaattgctg cgcaagttcc aaagaagcca gcgcaccata agtattttta
     9241 ttttcagcag caaatttttc tgccgccggg atgctatccg gtttgccttc gctcactgcg
     9301 gtaaccgcat tttgataggc aagagaagcg gagcgtgcag aatcaacctg atggctgttc
     9361 cagtagcgcc agccaatcag tgcgccaacg cccaaaatca ccccaacagc cagtgctttg
     9421 ccattttcag caaaaaagcg tttaaccgct tctacctggt cgttttcgtt ctcgtaaatt
     9481 tccacgctgt ccttctcctt ccttaaccca gtaacgtgcg caaatgcgcg gctacgctat
     9541 cctgcgcaac tgccgtttgc tcaccagagc gcaaatcctt cactactgct gtgccgttag
     9601 ccacttcaga ctcacccagc accacagcaa cgcgggcacc ccatttatca gcacgggcaa
     9661 actgtttctt aaagttgccg ccgccgtggt tggtcatcaa tttcacgccc ggtaattcat
     9721 cacgcagacg ctcagctaat gccatagccg cagattgtgt atcagcacct gaagccacca
     9781 ggtatatatc gacaacagga tcggctttaa attccggatt aacggcctgt actaacaata
     9841 caagacgttc gaggcccatc gcaaaaccga cagccggtgt tgcacggccg cccagttgtt
     9901 ccacaagacc gtcataacga ccgcctgcac acacggtgcc ctgggagccg agactgttag
     9961 tcacccactc gaaaacggta cggttgtagt aatccagacc acgcaccaga cgctggttta
    10021 cggtgtaagc gatccccgcg ctctcaagca gtttgcacag accggcaaaa tgctcacgag
    10081 attcctcgtc cagataatca cctaatgccg gagcgtcgtt gagaagcgcc tgcacttccg
    10141 gattttttga atccagcacg cgcagcgggt tagtgtacat gcggcgtttg cagtcttcgt
    10201 ccagcttttc tttatgctgc tcaaggaatg ccaccagcgc atcgcggtaa ttggcgcgtg
    10261 cttccagcga accgatagaa ttcagctcaa gagttacgtg ttcggaaata cccagcgcgc
    10321 gccaccagcg ggcggtgagc ataatcagtt cagcgtcgat atccggacct tgcagaccga
    10381 aaacttcgca gcccaactga tggaactgac gataacgccc tttctgcgga cgctcgtgac
    10441 ggaacatcgg cccgatatac cacagacgct gttcctgatt gtacagaaga ccatgctcga
    10501 tgccggcgcg tacacagccc gccgtccctt cagggcgcag agtcaggctg tcgccattgc
    10561 gatcctcaaa ggtgtacatc tctttttcaa ccacgtcggt gacttcacca atcgcacgtt
    10621 tgaatagcgg ggtctgctct acaatcggca agcggatttc actgtaaccg tagctgccga
    10681 gcacgttttt cagtgtgcct tcaatgcgct gccagatggc cgtttcgcca ggcaggtaat
    10741 cgttcatgcc gcgaatggct tgaatgtttt ttgccacgtt tattctcttt ctgaatataa
    10801 aaatgaaccc tcaacgcttc cctcaatgtt tcgggagcca tgcgggttca atcatacacg
    10861 ggaagcgagg cgcttcccat cacgttatta tttttcaacc tgctgaacgt caattcgacg
    10921 cgcttcgtcc agctgactgg ctttcgcacg aatgcgtgcc tccagctggt cgatcatatc
    10981 gttgttgtcc agacggtctt tgcgcacgcc atcttcatag agaccgcttt tcttgttgcc
    11041 gccggtgacg ccgagtgtag aaaccagcgc ctcacccggg ccattcacca cgcagccaat
    11101 aatcgaaacg tccatcggag tgatgatatc ttccaggcgt tgctccagcg cgttaactgt
    11161 accgataaca tcaaattcct gacgcgaaca ggtcggacag gcgatgaagt tgatacctcg
    11221 cgaacggata cgaagcgatt tcaaaatatc gaaaccgact ttgatctctt cgaccggatc
    11281 ggccgccagt gatacgcgca gcgtgtcgcc gatgccttca gacagcagca gacctaaacc
    11341 aatggcggat tttactgccc cgctgcgcgc gccacccgct tcggtgatcc ccagatgcag
    11401 cggctgatcg atttgttttg ccagcaaacg ataagactca acagcgagga agacgtcaga
    11461 cgctttcacg ctgactttga actgatcgaa gttcaggcga tcgagatgat caacatggcg
    11521 catggcggat tccagcaacg cctgcggcgt cggttcgcca tacttttctt gcagatcttt
    11581 ttccagcgat ccggcgttaa cgccaatacg gatcggaatg tttttatcgc gcgcacagtc
    11641 aaccaccatg cgaatacgct cttcattacc gatattgcca gggttaatac gcagacaatc
    11701 gacgccgtat tccgctactt tcagcgcaat gcgatagtcg aagtggatgt cagccaccag
    11761 cggcacgtta acctgctgtt tgatgagttt gaacgcttct gccgcgtcca tcgtcggtac
    11821 ggagacacgg acgatatcag cgccaacgcg ttccagcgcc ttgatttgat tgaccgttgc
    11881 ttcgacatct gtcgtacgcg tattggtcat ggactgtacg gcgatgggag caccatcgcc
    11941 aatcggcaca ttcccaacgt aaatacgtgt tgattttcta cgttgaattg gagcctggtt
    12001 atgcatgaaa aatctcccgc gttacccgtc tgttactgcg ccggtgattg ttcggcattg
    12061 agggtcagac gcgcaacctg gttagttctg ataaaacgac tcagatcgac aggtttccct
    12121 tgatactgga tctgtactgc ggctggcgca ccaattttca gtttgtatgg tgcctggccg
    12181 gttaagttca aattaccgtc tttacgctgc ataccgctaa acaatttttt accggtagca
    12241 tcagtgacct ccagccagca atcggcagta aagttcatca ccagcgcatt cggatcagcc
    12301 accggcgtgg tcacgccagc ctgatcggtt ggcaacggcg cagcaccatc tggcgttgtt
    12361 gctgccgttg gtgccggagt cgcggcggta tcaacatttg cctgcgaagg cgaaacaacc
    12421 gcattctgtt gcggatcaac agctggtgct ggcgcagtta cggcaggtgt ttgcgtgttg
    12481 gttgcggtag tatccacaga cgctggcggc gtggacgttg tagccgggtc tgtagttgtc
    12541 gacgtattta acggaacact ctgcccctgc tcgctattac tgctcagttc cgccgaagat
    12601 tgatcggcca tagtggtgat ctcttcctgc tgagctttgt ggtcttgcca ccaccaggca
    12661 ccgctcaggc cgataaccac aaacaacacc agccaggtga aggtcatcag ccagccgtcg
    12721 cgttttttgc ggcgtttacc gagggaaaaa ctctgcatcg gcgcaacttt tgcagcccgt
    12781 agtggagcct gcttttccag ccctggcagc agttcttctt ctggaatatg taccagacgc
    12841 gcataagagc ggatatatcc gcgcaggaat gttgaagcaa gatcggcggg tgccttatct
    12901 tcttcaatgt cgcgtaccgt ggaaaccttc aggcaaagtc gctcggcaac ggcctgctga
    12961 ctaagtccta gttgttcgcg agcattacgc aggcgagcgc cggtagtaag tgcttcattt
    13021 tggtcgtgcg tggcttcagt attcattcgc tgcaggtacg tttaaatgag aattgagatg
    13081 ccggtgaacc atcatgctca cccacaccgc gaaacatccg ttaagttaac cgttatcata
    13141 cagtataaga ccgtctgtgt gctcgtgaca aagcccacat tcactaaggc taacttactg
    13201 ttgcatcgtt acatactgcc ttaaagtcag caaaaacgca ccgttaatat tgaccagaca
    13261 attgcaactt aatacttcat tcataaaaca ttacgccacg gtacataaag taaccgtggc
    13321 gtaatggcta tcagaccgct ttaatgtcga tggcttcacc ctgcatccgt ttacgcagtg
    13381 tacgtttcgt acggtcgata acatcgcccg ccaactgacc acaggcagca tcgatatcat
    13441 caccacgcgt tttacgcaca atagtggtga aaccgtagct catcagcact tttgagaaac
    13501 ggtcgatacg gctgttcgag ctgcgtccat acggcgcacc cgggaacggg ttccacggga
    13561 tcaggttgat cttacacggc gtatctttca gcagttccgc cagttggtgc gcgtgttcag
    13621 tgccgtcgtt aacgtggtca agcatcacgt attcaatagt gactcggccc tgattggcgt
    13681 tggatttctc cagataacgg cgcaccgcag caaggaacgt ttcgatattg tactttttgt
    13741 tgatcggcac aatttcatca cgaatttcgt cgttcggcgc gtgtaaggaa attgccagtg
    13801 caacgtcgat catatcgccc agtttatcca gcgccggaac tacaccggaa gtggaaagcg
    13861 tgacgcgacg tttagacaga ccaaaaccga aatcatcaag catgatttcc atcgccggaa
    13921 cgacgttgtt caggttgagc agcggctcgc ccatgcccat catcactacg ttagtgatcg
    13981 ggcgctgacc ggtgactttt gctgcgccga cgattttcgc cgcacgccac acctggccga
    14041 taatttccga cacccgcagg ttgcggttaa agccctgctg ggcggtggaa cagaatttac
    14101 actccagcgc acaccccacc tgcgaagaga cgcagagcgt ggcacggtcg tcttcaggga
    14161 tatacaccgt ttcgacgcgc tgatcgccta cggcgatcgc ccatttaatg gtgccgtcag
    14221 atgaacgctg ttcttcaacc acttccggtg cgcggatctc cgccacctct ttcagtttgc
    14281 cgcgcaacac tttgttgatg tcggtcatct catcaaaatt gtcgcagcaa tagtgataca
    14341 tccacttcat cacctgatcg gcgcggaagg gtttttcacc taaatcttta aaaaactccc
    14401 gcatctgctg acgattgaga tccagcaggt tgatttttcc atctttggtg gtgacgtttt
    14461 caggtgtgac taattgttca gacatatgct attccggcct cgttattaca cgttatggcc
    14521 cctggagggt tgaaaaaaga aacgccccgg tgagcggctg ctcgtccggg ggcgctgcat
    14581 tgtacaaatt ctggcgcacg gatgccacgt ttgcacgcgg catttacgaa attattaacg
    14641 ggtgcgcggg cacacttcgc cttcgccgaa gaaataagcg atttcgcgag cggcagattc
    14701 gacggagtca gaaccgtggg taccattttc ggtcaggctg tcagcgtaat cagcgcgcag
    14761 agtaccggcc agtgcgtttg ccggattggt cgcgcccagc agatcgcggt gacgctgaac
    14821 ggcgttttca ccttccagca cggaaaccac gatcgggcca gaggtcatga attcaaccag
    14881 accatcaaag aacggttttc catcgtgttc agcataaaag ccacgagcct gttcaacggt
    14941 caggtgcagc attttggtgc cgacaatttt gaaccctgca gcttcaaagc gcgcaaagat
    15001 attaccaatg acgttttttg ctaccgcgtt cggtttgata atggaaaaag tacgttcaat
    15061 agccattttt acctctgtaa agtattcgtt gttgtctgta cctgcgtacg agatggcgcg
    15121 gattataaag agcaacaggg ccgttgacta ttgatgaagg taacattttt ttaaaataaa
    15181 atgagtttta gcaacaactc gacttttatg gtgctctcat cccatagcga tgacgttggc
    15241 aataaagaca attttgcgca ttttgctgaa aagtaagaaa ggatttttga caggttttac
    15301 agactttgcg attagcatga agattcacaa tttttgccgg tagaatcgtc aattcaacgg
    15361 tcattgcctg atgaaaacaa acagcaatac aattttggca tccgttacac aaggcatcat
    15421 ttatttgtaa atggccttcg ttaagcgaaa agacctgttg ctcgcaggcc cgccagcatg
    15481 caccgcaaag ctgacacttt tgaggatcca tcatcgggcg agcatcacta atctggggcc
    15541 ataaataatg taaccgacgt aatcctggtt gtatttgtgt catctgaaca tccgcacgct
    15601 gcacatggaa caaacgacgc cgggaaacat tcacctcact ggtatgtgga gataccacag
    15661 accagccttc ctcgccatat tgccgtaagc gttcgtttaa cagcaataat gcgcgctccc
    15721 atgccttcgc ctgactcaga tcaatcgcga tcgctgtgat cccctgctct ttatgccata
    15781 gcaaaagttc ttccagcgtc ggcgcaatag tggtgaaagg tgcgaccagg gtgtgattgc
    15841 gaaaataacg taaaggggaa ttaacgccac gaatagcctc ttgtggacaa ataaactggc
    15901 aaaccgcaca ctcaatacat tgttccgcgt cgaccgctat gccctcttct gtcagcgtta
    15961 gcgcctgtgc cgggcaagcc tcgacacagg catgacatgt tgcgaaacga aagcgttggc
    16021 gcgcgcattc cgcgccaatc gttatgttat gacttaacat tagccgatac taaaaaagac
    16081 aaaacgcagc ataacttcac tccccactag cattacgcta ccgaataacg ccgccgggat
    16141 attcttttta caaccaccga cagcaaagaa catcatccca atgacggaaa ttccccagct
    16201 tagtgtcagt atgggtttta actgtcccag catcgctaat ggcgcatgtg gcaatgtggc
    16261 tatctgcatc gcatttgccg ttaaatcccc tatccacaac ggctgaacca acaaacgcag
    16321 cagaaccagg agagcgatca ccaacacgca tccattacgc agggcatcaa tctgcggcaa
    16381 aattccgcta atacgaaaca ctgctaccag cgctgaaccg agaattccca ctgtacctaa
    16441 gaacataatc agcgtgttgt aatgctgcca ggtgataatc gacgtatgga tgtaaacttg
    16501 cgccatgcag aaaacatcaa caatcccgac cactgccgct agcataagca ggagttgcca
    16561 gccgcctttg cgtaacatga ccagtaatgt ggataaaccc aatagcgcca gatacagcgc
    16621 accaaagata atttcccgac tcagccatga actggaaaag tgacttagcg catgcatcgc
    16681 attaagtgga taacccaggt gtaaggtaga aatcagtaac cctacggccc ctgccaaaaa
    16741 agccaacagt aatggcggcc gcataaccgg gggggcgctg gaggtgatta aaatatgacg
    16801 atgaagaaac gccagccaca acgtcatacc gaccgatgcc tgtaacaaca gcgtaaaagc
    16861 cattaacggt aattcatgca tgatgtgacg cctccttttc cgcgccctga tgcggtttaa
    16921 tcaccagatt tggctgcgta atcgacgaat caggtaaacc tttcacatca ctgacatcgc
    16981 catacttttc acgcaactca tcaatagggc cgaacttaat cgcccccaac gggcacgttg
    17041 ccacgcaaat gggttgttct cctttagctt gcagatcaat acagaaatcg catttcgaca
    17101 tctgcccggt ctgctcattc atttgtggtg cgccgtaagg acaggaccaa gcgcagtaac
    17161 cgcaaccgac gcacttatca gtattcacgc gcacaatgcc gtctccggga cgcttatgca
    17221 tcgccatcgt cgggcaattt ttggtacaga tcggatccgc gcaatggtta caggaaacag
    17281 aaagggtata agcaaagaca ttatgactca caccgccctc tccagtggcg atgaagccac
    17341 ctccgttcac ttcatacacg cggcgaaaac gtcgcccaac ctcaaggttg tttttgtctt
    17401 tacaggccac ctgacacgct ttacagcccg aacaacgcga agagtcaata aagaacccta
    17461 attgtttgtc gctgacagat gcaaattctt taaattggct catgctttct caacctccac
    17521 cagcattgtt tgatgcgaat tccctttcgc cagcgccgta atgcgggtcg aactcagtac
    17581 attggcacag cctccgcgat cgataccttg tgcatccggt tgccaccatg cccccgcctg
    17641 catagcaact acgccaggga tgatacgggg ggttatttcc accgggataa tgctcacacc
    17701 acgatcgtta taaattttta cgctatctcc ttcgacaatc ccgcgctgtt ttgcatcttg
    17761 cggattcagc cagagttttt gcgtctgaac ttcttgcagc cacgggttag catactgggt
    17821 ggaattagca cggtttttcc ctttccaggt gatgagctgc agcgggtatt tcgccgtcag
    17881 tttatcttct ggcccttcaa aggcagggac atagtgcgaa agcgcgggaa tttctggatc
    17941 ttgcatatca aaaagacgct tagagaagat ctcaattttg ccggaaggcg tcgggaaagg
    18001 attattctgc ggatcgcgaa tatttgcttc gaaagcgatg tgcggcgcgc ttttgaaaag
    18061 atgtcgacgt gttttttgca gggtcgcgaa gtccggcaaa ttttcatccg gcatcgccag
    18121 acgagtttgc tcccagatat gctcgatcca ttgcttctca tcacgtcctt gactgaactc
    18181 ggcttcgaca cccagttttt tcgccacgtc acgcaaccag tcataatcgg tgcgacgttc
    18241 aaaatccggc tcaatcagtt tttctgacag aataagatag ctcgcagttc cccatgtttc
    18301 gccgatattc cagcgttcca taaagctggt ttccggcagc aacacatcgg catatttcgc
    18361 gctcggagtc atgaacagat cgctgagtac gatgaattct attttagatt cgtcttcaag
    18421 gagcttcgcg gcctggtgga cgtccgggtt ctggttcgcc agataattac ctgccagtga
    18481 gaacagcagg cggatattac tgtcgagttt atcgacacct ttaagcccat cttgcggcgt
    18541 aactttagaa gcatcatccg ccgcctgcat ccagttcata atcgagattt tcgcttgtac
    18601 cggattttct ggcatatctg gtccaacgca gaatttacga ttagaactac cgccgtaacc
    18661 tgccgcccag ccacctttga tgccgacgtt accggtaatc gaggccagta aagttgaacc
    18721 tcgggcggtg cgttcgccgc aagtatgtcg ctgtggcccc catccctgaa tcaacgctgc
    18781 gggttttgta gtggcgtaat cgcgtgccag ttgacgaatg gattgtgctg gaacgtgggt
    18841 aattttttct gcccattccg gcgttttgtg tatcccatct tttgcgccaa aaaggtaggc
    18901 aaccaacgat tcgttggctg gtacgccttc tggcatagag ttttcatcaa aaccgagagt
    18961 atatgtgtcg ataaatgtct tatcgtggag attctcgctg ataatgacgt acatcatcgc
    19021 gtccatcaac gcattgtccg tggtcggcag cagcgggatc cactgatcgg caagcgaaga
    19081 tacagtgtcg gaataacgcg gatctaccac gataaaacgc gtaccgtttt gcttcatttt
    19141 ttgaaaaaag taattggtgt gaccgaagat ggtttccgtt gggttatgcc cccagaggat
    19201 caccagtttg gtatccagaa gagtatccat cgagttaccg ctggccgcca cgccatacgt
    19261 gtaaggcgtt gccgccgcag tattacccag actaactgag tgataatatt caagataacc
    19321 accggttaaa tttaacagcc gacgcgccat cgcatcgcca gaaaatgttc caccagaaac
    19381 agccgtcccg acatggacat aacgtgaagc cgggccatat tgttgcgtga tccttttcag
    19441 gttatcggca attaaggtcg tcgcctcatc ccatgaaata cgctcaaatt taccctcgcc
    19501 tcgtttacct acccgcttca tcggatattt caaacggtct ggatgataaa caaatttacg
    19561 atagccgcga ccacgaacac aggcgcgcat aatcggcatt tgtggatcca gttcgttatc
    19621 gggaagcgtg gtaatttgcg tcaccacacc gtcgcgcata tgcgcccgaa tatcgcactt
    19681 accaccgcag tcaaatgtgc tacaggtggg cacgacttta tcagccaccg gagctgtaac
    19741 ttctggcgac gcatctgggg attgcgcctt tgcggtagaa gaaataaaag gcaaagtgat
    19801 aagcgcggaa gtcgcttgta aaaaactacg tcgtgatact tgcggaaatg taatgcgttc
    19861 cttttcgtct ggcgtatcca tcttgcgttc ccgaaaatga aaaaataaat attcacgtat
    19921 ttattcgaga acaatgctga taacaatcaa ataattagtc gtttaaaaaa acgtgagatt
    19981 attaaattat taatgcattc caaatataaa taccataatt agattaataa atattttgta
    20041 tatttcactg aaatgaaaaa tgtactgacg cgatttgccc tacgtcatcc atcaccagta
    20101 attgataatc acctttatcc gtcaaatgca gggtcacgtt gcgcccgcgt tcagttaacg
    20161 gttcgccatt cagaaaccac cagcgttcac ctgcccctcc acttgattgc aacggcaaag
    20221 tcgcttccgc tgcgcccggt aaacgtttaa taatctcgcc atcgcgaacg cctgtcagct
    20281 gcagcgggag ctgggcatcg tgaccgtatg gcgggcaaat tgtcgaggcc ggtggtaagc
    20341 gcacagcgcg acgttctgat gcgggcaacc agggttccag cggtagcggc cagacattta
    20401 tcatttcttg ttgcgcctga gggcaatcag cggcaacacg tttgccattt tcatccagcc
    20461 agatggggaa acgaatgcca ttaatgcctt cctgctccgg cagtaataga gttggcggct
    20521 gactcccgtc cagcagccag gttgccaggc ggcggcgaca gttaccgtca ccttccggca
    20581 aagactgtcc gcccggccag caaataacgc cacgagtgac tgagttcggg cgcgggtctt
    20641 ccggcagatt agcactgcgc gacagtaaga tattattgac ctgattcaac aatggtacgg
    20701 cactggcaaa gccaaactga ccaacaacgg gcgtgccgtc cggtctgcca gtccagatcc
    20761 caatgacata gcgagcgtta accccaatcg cccaggcgtc acgatagcca tagctggtgc
    20821 ccgttttcca tgccagtggg gcgacgcgcg gcaaggcacc atccggcaag ggttgcgctt
    20881 catcagccat aatccggcga atgatccacg cagcctccga cgacattaaa ggccgttcaa
    20941 gcagcggatc gtcaggctgt aagcgcaatt tgcctgcctt gccctggcga gcaaacgcgg
    21001 tatacgctgc cgccatatct tccagttttg caccagcgcc gccgagaatg agcgaaagat
    21061 tcggcgccgc accgttgggc aaatataacg gcaatccaac attgcgtaac tttgccgcaa
    21121 accgtttcgg tccataggct tccagtacct gcacagcagg taaattcagc gagcgcacca
    21181 gcgcctcgct catgctgatc gggccatgaa aaccgctatc aaagttacct ggtcgataat
    21241 caccggtgcg ccgggggacg tcttgcaaca gcgatgccgg atggatcaag ccttcatcca
    21301 gcgccagacc gtaaacaaac ggtttgagca ccgaccctgg cgatcggatc gcattgacca
    21361 tatcaacatg accaaagcgt gaatcatcgt tgagatcaac cgaacccacc cagccgcgaa
    21421 cacgcatatc ggtatgatca accacgatca tcgccagtga gctgcgcggt ggtaatcgtc
    21481 ctttccagtt ttgcgccagt tcttccagac gtcgttgaag accggcatcc agcgtagtaa
    21541 cgattttgtc gcttttgctt ttaccgagca tcatgcgcga aaacagcggt gccagttgcg
    21601 gcatttgtcg gggtgccagc cagatgggtt cttcccttga ctcttttacc tgctcacggg
    21661 accacacacc ttgcacggcc atccgttcga gcactttatt acgcgcgact tcggcacgct
    21721 ccggccaacg atccgggcga agacggctgg gcgcttgcgg caaaaccgcc agcattgccg
    21781 cctcggaata gcttaaattc gcaggcgact ttccgagata agcccaactt gccgcaccga
    21841 tcccctgcaa cgtaccgcca aacggagcgc ggttaagata caaggtcaga atttcacgct
    21901 tagacagatg ccattccagt tgcaacgcgc gccagagctg gcgaattttg ccgccaaatg
    21961 ttttggggtg aggatcaagc agacgagcaa cctgcatagt gagcgtgctg ccaccggaaa
    22021 taacccgtcc cgaagtgaga tcttgccatg ctgcgcgcgc caccgagaat ggattcaccc
    22081 ccggatgctt ccagaaccag cgatcttcat aattgatcag cgcttcaagg taacgtggag
    22141 aaacatcttc gattgttacc ggataacgcc agatgccgtc agcatcggcg aagcgccaga
    22201 gcggcgtacc atcctgcgcc acgaccactc gtgcgggatt gacttcatgc agcggtagag
    22261 gccagagttt atctgctccc cacgctgcaa gaaaaaggag aaatggcgca gctgccagcg
    22321 ttatccagca gccgcgtttg gttaacaagc gaggcattta cggtctgaca atcagcaaat
    22381 cttcagccgc gccggtcgcc cgccattggg gaacatacat tgattccacc atcggttgcg
    22441 gtacctgata cgttcccggc gtcaccgccc gcgccagata caccagcgtt accggttggt
    22501 attcatcaac ggcaaccgcc gccacaaagc gatcgtcacg gaactcaatg tgcttaatac
    22561 tcgcctgctg catctggttc agtaagttct gcacttcgcc accactttgc tccaggctgg
    22621 cgctaccgtt cgccagattc tggttttcca gttccagacc cgcaggcagc agatccacga
    22681 ctaacgcatc cggcacgctg ttactggctt ttacctgcaa ccagaccagc accagatcgc
    22741 cgctacgtaa cgagtccagc gatttgctct taccatcagt accaagaata tgacgctcaa
    22801 tttgcagcac attgttcgcc ggtaaaggtg cggattgcgg ataaccgctg gcatccacac
    22861 gcagccataa cggctgctcg ccactgttgg tcacctgcaa ggtggcaagt tgatcgctat
    22921 tcagattgct gttttgcgct ttctcgcctg tcagcggctc agttgagaaa gaggtttgcg
    22981 cctgccattt acctggtaaa tcctgaatcg tacgggcagc caggaacaac gcgttacttt
    23041 cctgcgtcga tagccagcgt tcaccaaacg cctgctgcga aagtgtgttc agcagggagt
    23101 actgctcatc gggtagcagt ttgttctctt ccagcaagga gagcatcaac gcgttgtcgc
    23161 gcagtggact accgtaatca cccagccata tccgctcatc actattacgc ggcgttttta
    23221 gcgccagcgc aatcgcttct tcacaacgcg tcgcatcacc catggttttc agcgcaacgc
    23281 caagttgcag cagcggtaaa ccagaagcgg catctgcacg atgctcccag atttcacgca
    23341 gcgcacccag cggagccttt tgctgacggg ccaacaccag cgcagcgtat gactgtacgg
    23401 cgaatttact ggctttgaga ttatccgcgt acgggatcga catcatgccc ggatcttgta
    23461 aatagcgcag cagacgctca ttaccccggt taatggcgtc tgtcggcacg ctgtaaccct
    23521 gttcgcctgc gcggaccagg aaatccatca cgtaagccgt cagccagtac tcttcgtcac
    23581 cgtttttatc ccacagcgca aagccgccgt tatcacgttg catttgcagc aaacgggaaa
    23641 tgccgatatc gaccgatgca cggcgtttct catcactgtc gcctttgatg cccaacgcct
    23701 gcagttgggc tgcgttggta taaagtgacg ggaacaggcc gctggcggtt tgctcaagac
    23761 agccgtacgg atacgctttt aactctttga tataacgtgc gatgttcagc ggtggtttgc
    23821 cgctcaacaa caattgccct tccagcgtaa caggtgagaa gttttgcaat ccatccgccg
    23881 gaatcgccca tgtctcacca ggctgtaacg ccgtaccgta attaaccgtt tgtgccggga
    23941 acgccggacg gacgccgatt ttccactgct tatgctgatc ggcaacggtt tcacccggta
    24001 acgctaaccc gctaatggtg gcctgaattt ctccatcgcc ataacccggc aatgctcgca
    24061 ccgggataaa cagcgtagta cgcacacctg gcgctaattc aacggctgcg ggtgaatcgc
    24121 tgaccagttc aagcaaacca ctggcggtca gggcaacgtt cagtttttgc ggtttatcgg
    24181 taagattagt gatatccagc gtcagacgcg aggtatcgcc actcgccata aagcgcggca
    24241 tgttcagttc agcaatcacc ggtgcggcaa ctatcacttt gctttcgttg ctaccgaagt
    24301 catctgccgt ccaggcttgc gccatgacgc gcaattcacc gttaaaatcg ccaatcggca
    24361 gtgtaaccga gccttcgccc tgttcgttga gcgttaccgg cagcgcctgc tgcgcgacaa
    24421 tattgacgtg attgaccggc ggtttaccac cacgtttcag ctcatcacca tcgccaccga
    24481 aacgcagagc tgccagacgc ccctgacctt caataacctg accgtaaata tcgtaaatgt
    24541 ctgcgccata gcgtttctga ccaaagaacg cctgccacgg atctggcgtg acgtagtcag
    24601 taatattcag cacaccacta tcaacggcag acaccagcac attcacctgt ttaggcttct
    24661 cgccattttt agtgctggct ttaattttca cggttaatgg ttgattggga cgcatttttg
    24721 ctggtgtttc cagcgccaga tcgaggcgac ggttttcatc accaagcggc agatgcaaca
    24781 caccaaccgc gcgttttggc gtcgcggagc gagatttatc gccaggacgc accaccagcg
    24841 tactcaaata aagatcatga cgattccagg ttttatcgac cggaatcgtc agatccagcc
    24901 cttgagccgg aacatcaatc tcttgccacc acagcggccc ttcactggac tcgaccatcg
    24961 cataaccttt acccgccgtt ggcgcggcaa tatgcaactt aatggtatcg ccagggcgat
    25021 aactggcttt atctagtttc agcgtgacac ggtcgggtcg cacggcaccg ctcccgtcgc
    25081 tgttgtcctg ccagctatag ccagcccaga aacgcacact actgaccgct tcattcggcg
    25141 ctttgacttc cagacgataa gcgccccact ctaccgggaa actgaccttg ccggtttcgt
    25201 ccgctttcag atccagagtt tgttcatttt caatcagatc tttttgatca aactgtgatt
    25261 gccagccttc atcttctgac cagttccagt agtaatcgcg gcgttcgcga atcaggcgca
    25321 cctgcaagcc cgacacggct tttttcacgc cttgcgcatc gctataaacg atgtcaaaag
    25381 cggcgttact gccttcatca acaatgggct gtttcaccgt gctgtcagtg cgataatcgt
    25441 aaaccgattt cgaggcgaac tgcggacgga tccccggcaa tgcatcggca ggccagatag
    25501 cctgctcagc gcgacgcgtc actgggcgac cgcccgattc cagcaaactg ccctggaaaa
    25561 taacctgtaa tggggaatgc gtttccttcc actggctttc tgtagaaact tcgccgcgcc
    25621 ctttatcatc cagcgtcaac tgaacttcat ccagcgtgcg ggaaaggttt tcggcagcta
    25681 tatcgccgaa ttcaaaacca ggtaaggctg acacagcctc acgcagtggg cgcaggaaaa
    25741 gttgcccttg caaagtatta ccattagcag gtgcaccgta caggtagtat cccaccacgg
    25801 agaatttcac ttcatcattc ggcgtaagcg gggttttctc accggtcaga ttcagcgcca
    25861 tacgctctgg cataaaatct tcgacgtgga aatcccacat ccgatactgg ttatcgcccg
    25921 tgttagcgcg aatatgccac ataccggttg ccgcattgct atcgagtggc caggtaaagt
    25981 ggtagaggcc attttccggc tgactaacga cgctcctgag tacctgccca tcgggtttaa
    26041 tcacttctaa cttgatgggt tgattgggca acgctttacc gtctgcatca cgcagcaaac
    26101 cattgaggat gaccgtttca cccgggcgat aaagatcgcg cgggccaaac atgaaaaact
    26161 gtttgctata gcctggcgcg ccagcaatgt taaattctgc taagtccagc gccggaagtt
    26221 ttaaatcgag tagcgttgtc tgaccgtctt tacgtgccag caataatgcg gcgtttttat
    26281 cattttccag ctgcacatgc ccctgagcgt cactggttgc ctgagtcaga gtctgccctt
    26341 tctcatttaa taaagagact tcaattcctt gctgggccgc gccgttttcc agactttggg
    26401 taaagatatc cagacgattg tgataacggt gagctgaaac gccgatatca cttaacgtaa
    26461 acagcgtcgc gggattactg taatcgtaac gtccagcctg attcatcaca gccagataca
    26521 cgcccgcctg ctgaagcggt ttgatatcgc ccagcggcag caataatttt tcacgggtgt
    26581 tacgcgcagg attgagatca aaccgtccgg tgtagaccag atccgccatc tgcagcagtt
    26641 tgtctgactg ccagttcgcc agcgaattgc ggtattccca ttggctaatg aatgctggca
    26701 gagattctgg cttaacgcgg aagaagttaa catcgacatt attaacgttg agcgccatta
    26761 ccggcagccc ttcaacgact ttaccaggca gcagcgaacc acggctggca aaaccgacgc
    26821 tgggttggat gtcgcgggta gttatagttt tttcgtaatc tttactgaag gttgcgttgt
    26881 tgagtgcttt gacctccttg ccaatagtaa cgatcaaatc acgtttcggt tcgaggtggc
    26941 gtaaacgcag ctctttaaga ttatctgaca gctcccaggc accatccact ttgccgcttt
    27001 ttttatcgac gacatgaata acgcgtgaga aatcctgatc cgggtcgaga gggatggaga
    27061 acgtcagcac cagcgtggct gcaccatcaa gttggacttc agagagatcc agcaatgtca
    27121 gcgccttacc ggcactctgt tgtgccagtt tttgtcgctc cggcacggag agttttgctg
    27181 aactcgcgtt ttctgaagag gccgctttag taacttcaga aggcgcatct tttttcaccg
    27241 ctgttggcgc gttatcgttg ttgtcgcacc ctgccagcgc cagcattagc atgcaggcgg
    27301 ctacgcgtaa ctttttcatt tttcatccct gccacaatgg cccgttagca acgtcgaata
    27361 attattatgc gtgagaattt acgttatgta attcagtatc acgcccatac aatccgaatt
    27421 ttagtgagaa ttgtggctca gacagccaaa tcagcctctt ttgcttgtcg cttttcacta
    27481 aacatccgac aatttagcct aaccccggca aaaatggaga tgcctatgtc cacgacatgg
    27541 tttgtaggag ccgactggct cgccgaacat attgatgacc cggaaattca gattatcgat
    27601 gcccgcatgg cgtcgcctgg acaggaagat cgtaacgttg ctcaggagta tctgaatgga
    27661 catattcccg gcgcagtgtt ttttgatatc gaagcgcttt ctgatcacac ttccccgctt
    27721 ccgcacatgc tgccgcgccc ggaaacgttc gccgtggcga tgcgtgaatt aggcgttaac
    27781 caggataagc acctgattgt ctatgacgaa ggtaatcttt tctcagcccc acgagcatgg
    27841 tggatgctgc gcacctttgg tgtagagaaa gtgtcgattc tggggggtgg acttgcaggc
    27901 tggcagcgcg atgatctgtt gttagaagaa ggtgcagtag agctgccgga aggagagttt
    27961 aacgccgcgt ttaatcctga agcagtggtg aaagtaaccg atgtgttatt ggcaagccat
    28021 gaaaatacgg cgcaaattat tgatgcccgc ccggctgcac gttttaacgc agaagttgat
    28081 gaacctcgcc caggtttacg tcgcggacat attcccggtg cattgaacgt tccgtggacg
    28141 gaactggtgc gcgaaggcga actaaaaacg accgatgaac tggatgcgat attttttggt
    28201 cgcggcgtca gctatgacaa accgattatc gtcagctgcg gctctggtgt aacagcagcc
    28261 gtggttttgt tggcactcgc gacgctggat gtgccaaacg taaaattata cgacggtgcg
    28321 tggagtgaat ggggcgcacg ggcagattta ccggttgagc cattgaaata agctttatac
    28381 aggcaataaa aaaccgccga aaataaaaaa ccgccgaatt tggcggtttt ttattgctgg
    28441 tctggttcgc ggcctttcca gcaggttgac ttgtgttgcc ggagcaacgc aggcacccca
    28501 cagtgttgaa atactctctt ttatgcctgg ctgtgtcaag ataataaaca gagtaattat
    28561 tcaaacataa gagtattatt cttaatggcc cttttcgccg tctcgcaaac gggcgctggc
    28621 tttaggaaag gatgttccat gaccgtaaat acaggcaccc cacagtgctt gctatcgcgg
    28681 caatatcgcc agtggtgctg tcgtgatgcg gtcttcgcat ggaccgcaca atgaagatac
    28741 ggtgctt
//
LOCUS       DAZKFU020000034        28667 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZKFU020000034 DAZKFU020000000
VERSION     DAZKFU020000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.034"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..617)
                     /locus_tag="ACHOQQ_RS05955"
                     /old_locus_tag="pgaptmp_001191"
     CDS             complement(<1..617)
                     /locus_tag="ACHOQQ_RS05955"
                     /old_locus_tag="pgaptmp_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4469467.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA"
     gene            complement(688..2802)
                     /gene="fusA"
                     /locus_tag="ACHOQQ_RS05960"
                     /old_locus_tag="pgaptmp_001192"
     CDS             complement(688..2802)
                     /gene="fusA"
                     /locus_tag="ACHOQQ_RS05960"
                     /old_locus_tag="pgaptmp_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGU4469468.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2899..3369)
                     /gene="rpsG"
                     /locus_tag="ACHOQQ_RS05965"
                     /old_locus_tag="pgaptmp_001193"
     CDS             complement(2899..3369)
                     /gene="rpsG"
                     /locus_tag="ACHOQQ_RS05965"
                     /old_locus_tag="pgaptmp_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGU4469469.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3466..3840)
                     /gene="rpsL"
                     /locus_tag="ACHOQQ_RS05970"
                     /old_locus_tag="pgaptmp_001194"
     CDS             complement(3466..3840)
                     /gene="rpsL"
                     /locus_tag="ACHOQQ_RS05970"
                     /old_locus_tag="pgaptmp_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGU4469470.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3966..4253)
                     /gene="tusB"
                     /locus_tag="ACHOQQ_RS05975"
                     /old_locus_tag="pgaptmp_001195"
     CDS             complement(3966..4253)
                     /gene="tusB"
                     /locus_tag="ACHOQQ_RS05975"
                     /old_locus_tag="pgaptmp_001195"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGU4469471.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4261..4620)
                     /gene="tusC"
                     /locus_tag="ACHOQQ_RS05980"
                     /old_locus_tag="pgaptmp_001196"
     CDS             complement(4261..4620)
                     /gene="tusC"
                     /locus_tag="ACHOQQ_RS05980"
                     /old_locus_tag="pgaptmp_001196"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGU4469472.1"
                     /translation="MKRIAFVFSTVPHGTAVGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4620..5006)
                     /gene="tusD"
                     /locus_tag="ACHOQQ_RS05985"
                     /old_locus_tag="pgaptmp_001197"
     CDS             complement(4620..5006)
                     /gene="tusD"
                     /locus_tag="ACHOQQ_RS05985"
                     /old_locus_tag="pgaptmp_001197"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGU4469473.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(5006..5728)
                     /gene="yheO"
                     /locus_tag="ACHOQQ_RS05990"
                     /old_locus_tag="pgaptmp_001198"
     CDS             complement(5006..5728)
                     /gene="yheO"
                     /locus_tag="ACHOQQ_RS05990"
                     /old_locus_tag="pgaptmp_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4469474.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5895..6707)
                     /gene="fkpA"
                     /locus_tag="ACHOQQ_RS05995"
                     /old_locus_tag="pgaptmp_001199"
     CDS             complement(5895..6707)
                     /gene="fkpA"
                     /locus_tag="ACHOQQ_RS05995"
                     /old_locus_tag="pgaptmp_001199"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4469475.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6928..7146
                     /gene="slyX"
                     /locus_tag="ACHOQQ_RS06000"
                     /old_locus_tag="pgaptmp_001200"
     CDS             6928..7146
                     /gene="slyX"
                     /locus_tag="ACHOQQ_RS06000"
                     /old_locus_tag="pgaptmp_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGU4469476.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7195..7785)
                     /gene="slyD"
                     /locus_tag="ACHOQQ_RS06005"
                     /old_locus_tag="pgaptmp_001201"
     CDS             complement(7195..7785)
                     /gene="slyD"
                     /locus_tag="ACHOQQ_RS06005"
                     /old_locus_tag="pgaptmp_001201"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4469477.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7880..8080)
                     /gene="yheV"
                     /locus_tag="ACHOQQ_RS06010"
                     /old_locus_tag="pgaptmp_001202"
     CDS             complement(7880..8080)
                     /gene="yheV"
                     /locus_tag="ACHOQQ_RS06010"
                     /old_locus_tag="pgaptmp_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGU4469478.1"
                     /translation="MAIRKRFIAGAKCPVCQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8090..9895)
                     /gene="kefB"
                     /locus_tag="ACHOQQ_RS06015"
                     /old_locus_tag="pgaptmp_001203"
     CDS             complement(8090..9895)
                     /gene="kefB"
                     /locus_tag="ACHOQQ_RS06015"
                     /old_locus_tag="pgaptmp_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGU4469479.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHTDT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9895..10446)
                     /gene="kefG"
                     /locus_tag="ACHOQQ_RS06020"
                     /old_locus_tag="pgaptmp_001204"
     CDS             complement(9895..10446)
                     /gene="kefG"
                     /locus_tag="ACHOQQ_RS06020"
                     /old_locus_tag="pgaptmp_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGU4469480.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10577..12490
                     /gene="yheS"
                     /locus_tag="ACHOQQ_RS06025"
                     /old_locus_tag="pgaptmp_001205"
     CDS             10577..12490
                     /gene="yheS"
                     /locus_tag="ACHOQQ_RS06025"
                     /old_locus_tag="pgaptmp_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4469481.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12490..13512
                     /gene="yheT"
                     /locus_tag="ACHOQQ_RS06030"
                     /old_locus_tag="pgaptmp_001206"
     CDS             12490..13512
                     /gene="yheT"
                     /locus_tag="ACHOQQ_RS06030"
                     /old_locus_tag="pgaptmp_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4469482.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13506..13724
                     /gene="yheU"
                     /locus_tag="ACHOQQ_RS06035"
                     /old_locus_tag="pgaptmp_001207"
     CDS             13506..13724
                     /gene="yheU"
                     /locus_tag="ACHOQQ_RS06035"
                     /old_locus_tag="pgaptmp_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGU4469483.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13778..14647
                     /gene="prkB"
                     /locus_tag="ACHOQQ_RS06040"
                     /old_locus_tag="pgaptmp_001208"
     CDS             13778..14647
                     /gene="prkB"
                     /locus_tag="ACHOQQ_RS06040"
                     /old_locus_tag="pgaptmp_001208"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGU4469484.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14702..15106)
                     /gene="yhfA"
                     /locus_tag="ACHOQQ_RS06045"
                     /old_locus_tag="pgaptmp_001209"
     CDS             complement(14702..15106)
                     /gene="yhfA"
                     /locus_tag="ACHOQQ_RS06045"
                     /old_locus_tag="pgaptmp_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGU4469485.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15408..16040
                     /gene="crp"
                     /locus_tag="ACHOQQ_RS06050"
                     /old_locus_tag="pgaptmp_001210"
     CDS             15408..16040
                     /gene="crp"
                     /locus_tag="ACHOQQ_RS06050"
                     /old_locus_tag="pgaptmp_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGU4469486.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16091..18181
                     /gene="yhfK"
                     /locus_tag="ACHOQQ_RS06055"
                     /old_locus_tag="pgaptmp_001211"
     CDS             16091..18181
                     /gene="yhfK"
                     /locus_tag="ACHOQQ_RS06055"
                     /old_locus_tag="pgaptmp_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGU4469487.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTAPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRIIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKT"
     gene            complement(18248..19468)
                     /gene="argD"
                     /locus_tag="ACHOQQ_RS06060"
                     /old_locus_tag="pgaptmp_001212"
                     /gene_synonym="dapC"
     CDS             complement(18248..19468)
                     /gene="argD"
                     /locus_tag="ACHOQQ_RS06060"
                     /old_locus_tag="pgaptmp_001212"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGU4469488.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     YQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19554..20117)
                     /gene="pabA"
                     /locus_tag="ACHOQQ_RS06065"
                     /old_locus_tag="pgaptmp_001213"
     CDS             complement(19554..20117)
                     /gene="pabA"
                     /locus_tag="ACHOQQ_RS06065"
                     /old_locus_tag="pgaptmp_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGU4469489.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20149..20751)
                     /gene="fic"
                     /locus_tag="ACHOQQ_RS06070"
                     /old_locus_tag="pgaptmp_001214"
     CDS             complement(20149..20751)
                     /gene="fic"
                     /locus_tag="ACHOQQ_RS06070"
                     /old_locus_tag="pgaptmp_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGU4469490.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20741..20908)
                     /gene="yhfG"
                     /locus_tag="ACHOQQ_RS06075"
                     /old_locus_tag="pgaptmp_001215"
     CDS             complement(20741..20908)
                     /gene="yhfG"
                     /locus_tag="ACHOQQ_RS06075"
                     /old_locus_tag="pgaptmp_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGU4469491.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(21013..21585)
                     /gene="ppiA"
                     /locus_tag="ACHOQQ_RS06080"
                     /old_locus_tag="pgaptmp_001216"
                     /gene_synonym="rotA"
     CDS             complement(21013..21585)
                     /gene="ppiA"
                     /locus_tag="ACHOQQ_RS06080"
                     /old_locus_tag="pgaptmp_001216"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGU4469492.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21856..23037
                     /gene="tsgA"
                     /locus_tag="ACHOQQ_RS06085"
                     /old_locus_tag="pgaptmp_001217"
     CDS             21856..23037
                     /gene="tsgA"
                     /locus_tag="ACHOQQ_RS06085"
                     /old_locus_tag="pgaptmp_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGU4469493.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23299..25842
                     /gene="nirB"
                     /locus_tag="ACHOQQ_RS06090"
                     /old_locus_tag="pgaptmp_001218"
     CDS             23299..25842
                     /gene="nirB"
                     /locus_tag="ACHOQQ_RS06090"
                     /old_locus_tag="pgaptmp_001218"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGU4469494.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLIGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25839..26165
                     /gene="nirD"
                     /locus_tag="ACHOQQ_RS06095"
                     /old_locus_tag="pgaptmp_001219"
     CDS             25839..26165
                     /gene="nirD"
                     /locus_tag="ACHOQQ_RS06095"
                     /old_locus_tag="pgaptmp_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGU4469495.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26291..27097
                     /gene="nirC"
                     /locus_tag="ACHOQQ_RS06100"
                     /old_locus_tag="pgaptmp_001220"
     CDS             26291..27097
                     /gene="nirC"
                     /locus_tag="ACHOQQ_RS06100"
                     /old_locus_tag="pgaptmp_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGU4469496.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27116..28489
                     /gene="cysG"
                     /locus_tag="ACHOQQ_RS06105"
                     /old_locus_tag="pgaptmp_001221"
     CDS             27116..28489
                     /gene="cysG"
                     /locus_tag="ACHOQQ_RS06105"
                     /old_locus_tag="pgaptmp_001221"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGU4469497.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6942 a         7704 c         7229 g         6792 t
ORIGIN      
        1 gcacgctctg gttccggaat gtaagaatcc aggaagccag ccagttccag gattttcgct
       61 tcccactctg cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg
      121 tcgcccggga agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc
      181 agctcttcgt catcaaccat gtcgcatttg ttcaggaaca cgatgatgta cggaacgcct
      241 acctgacgac ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca
      301 actaccagga tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg
      361 gcgtgccccg ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcata ttcaacgtga
      421 gaagtgttga tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat
      481 gcacgagcag caccaccgta ggttttagcc agtacggtgg tgattgcagc ggtcagagta
      541 gttttaccgt ggtcaacgtg gccgatagta ccaacgttaa cgtgcggttt tgtacgttca
      601 aatttttctt tagacacggc tatattcctt actatagtgc tctccccttc aggagagagc
      661 acgggacttt ggtattaacc cttaggctta tttaccacgg gcttcaatta cggcctgagc
      721 aacgttactc ggcgcttcat catacttcag gaattccata gtgtatgatg cacgaccttt
      781 ggtcagagaa cgcagctgag ttgcgtatcc gaacatttca gacagcggta cttcagcgtg
      841 gatcttaacg ccagtaactt cagattcctg acctttgagc ataccacgac gacggctcaa
      901 gtcaccgata acgtcaccgg tgttctcttc cggagtttct acttcaacct tcatgatcgg
      961 ctcaagcaga actggtttcg ctttcttaaa gccttcttta aaggcgatag aagcagccag
     1021 tttaaacgcc agttcagagg agtcaacgtc atggtaagaa ccgaagtgca gacgaatacc
     1081 catgtctact accgggtagc ctgccagcgg acctgctttc agctgttcct ggataccttt
     1141 atcaacggcc gggatgtatt cgccagggat tacaccacct ttaatgtcgt tgatgaactc
     1201 gtagcctttc gggtttgaac ccggctccag cgggtacatg tcgataacaa catgaccata
     1261 ctgaccacga ccaccagact gtttcgcgtg tttaccttca acatcggtaa ctttctggcg
     1321 gatagtttca cggtaagcaa cctgcggttt acctacgttc gcttcaacgt tgaattcacg
     1381 cttcatacgg tcaacgatga tgtcgaggtg cagttcgccc atacccgcaa tgatggtctg
     1441 gttagattct tcgtcagtcc atacacggaa agacgggtct tctttagcca gacggcccag
     1501 agccagaccc attttttcct ggtcagcttt ggttttcggt tcaactgcga tggagattac
     1561 cggctcaggg aattccatac gttccagaat gatcggcgca tccgggtcac acagggtgtc
     1621 accagtggtt acgtctttca gaccgatagc agcagcgatg tcgcccgcgc gaacttcttt
     1681 gatctcttca cgtttgttag cgtgcatctg aacgatacga ccgaaacgct cacgtgcagc
     1741 tttcacggag ttcagtacgg tatcaccaga gttaaccaca ccggagtaaa cacggaagaa
     1801 ggtcaggtta ccaacaaacg ggtcggtagc gattttgaac gccagtgcag agaacggctc
     1861 gtcatcactt gcgtgacgtt cagccggagt gtctttaccg tcgtccagga taccgttgat
     1921 cgcaggtacg tcaaccgggg atggcaggta atcaattacc gcatccagca tcgcctgaac
     1981 acctttgttc ttgaacgcag aaccacaggt taccaggatg atttcgttgt tcagaacgcg
     2041 ctgacgcaga gcacctttga tttctgcttc agtcagttct tcaccaccca ggtatttttc
     2101 catcagctct tcagaagctt cagctgcgga ttcgatcagg ttctggtgcc attcgttagc
     2161 cagttcaacc atgtctgccg ggatatcttc gtattcgaag gttacgccct ggtcagcgtc
     2221 gttccagttg atagctttca ttttcaccag gtcaacaaca cctgtgaaat gttcttcagc
     2281 accaatcgcc agctgcagcg gaaccgggtt cgcgcccaga cgggttttga tctggttaac
     2341 aactttcagg aaattcgcac ccatgcggtc cattttgtta acgaacgcaa tgcgcggaac
     2401 tttatatttg tttgcctgac gccatacggt ttcagactgc ggctgaacac caccaactgc
     2461 gcagtaaacc attaccgcac catcgagaac acgcatggaa cgttctactt cgattgtgaa
     2521 gtcaacgtgc cccggggtgt cgatgatgtt gatgcgatgc ggctcatact gcttagccat
     2581 accagaccag aatgcagtag tcgcagcgga agtgatggta ataccacgtt cctgctcctg
     2641 ctccatccag tccatggttg cagcgccgtc atgaacttca ccgattttat ggtttacacc
     2701 ggtgtagaac agaatacgtt cggtagtagt ggttttaccg gcgtcgatgt gcgcactgat
     2761 accgatgtta cggtagcgtg cgatgggtgt tgtacgagcc atttgtttcc tcgtttatct
     2821 tttaggcgtt caatttaagt agcccaaagc gggctgctta ctggaagcgc ccgcctggtg
     2881 actaaaactc cgaagggatt accaacggta gtgtgcgaac gccttgttgg cttcggccat
     2941 acggtgaacg tcttcacgtt tcttaactgc agtacctttg ttttctgcag catcagaaag
     3001 ttcgttcgcc aggcgcagag ccatggattt atcaccgcgt ttacgagcag cttcaacgat
     3061 ccaacgcatt gccagagcat tacgacgaac cggacggact tcaactggta cctgataagt
     3121 agaaccacca acgcggcgag acttaacttc tacagtcggg cgcacgtttt cgagagctac
     3181 ttcgaatgct tccagttcag atttaccaga gcgctgagcc agggtctcca gcgcgctgta
     3241 tacgatagat tcagcagtag attttttacc atctaccatc aggatattta caaatttagc
     3301 cagcagttct gatccgaact tcggatccgg cagaatttta cgctgaccaa tgacgcgacg
     3361 acgtggcatg gaaatactcc gttgttaatt caggattgtc caaaactcta cgagtttagt
     3421 ttgacattta agttaaaacg tttggcctta cttaacggag aaccattaag ccttaggacg
     3481 cttcacgcca tacttggaac gagcctgctt acggtcttta acgccggagc agtcaagcgc
     3541 accacgtacg gtgtggtaac gaacacccgg gaggtcttta acacgaccgc cacggatcag
     3601 gatcacggag tgctcctgca ggttgtgacc ttcaccaccg atgtaggaag tcacttcgaa
     3661 accgttagtc agacgaacac ggcatacttt acgcagcgcg gagttcggtt ttttaggagt
     3721 ggtagtatat acacgagtac atacgccacg tttttgcggg catgcttcca gcgcaggcac
     3781 gttgcttttc gcaactttgc gagcacgtgg tttgcgtacc agctggttaa ctgttgccat
     3841 taaatagctc ctggttttag cttttgcttc gtaaacacgt aataaaacgt cctcacacaa
     3901 tatgaggacg ccgaatttta gggcgatgcc gaaaaggtgt caagaaatat acaacgatcc
     3961 cgccatcacc aggccatctg gctggagtgc ttaaccgtaa gtctgacgaa atcagtatag
     4021 tcaatgggaa tgatgtcgtt cgaaatttga ccagtcaaac cgcgggcaat aaggtcttcg
     4081 ttcagggcat agaccttaat gggggcatta cgcagacttt caaggtagcg gttaccgtca
     4141 actgcggcag ttacgccatc ttgcaataat agcagttcgt cgccttcact gagcagacgc
     4201 agcagcgcag caaaatccgt cagccagggt gaacgatgta acgtgtgcag cataaacgcc
     4261 tcaaaacctc aaaataacat cgtagttggc gagttcgcgg cgtaaggcat ctgcttcgag
     4321 cggcgtggct tcgacaacaa agggggtctg cggatctaac ccgcgttcgc gcagtgaagc
     4381 cgcacaaacc cagcactgtt caatgtcgta cagacccaac aatttaaaag tggcaatgta
     4441 atcacgcgcc agcactgcat cgggcttttg tcctggcagt agctgaaaaa cgccatcagc
     4501 aataaagaag acggccagct cgtcagttaa cgcggaagtt gccagtaaag catctaaacc
     4561 ttcccgcccg acagcggtac catgaggcac agtagaaaaa acaaacgcaa ttcgtttcat
     4621 cagaactgta ccaccctgtc acaggtcagc gaggcttccg ccagcgcgcc aagtccgctt
     4681 aaggtaaatc cctgctgaag gtttgacgaa gccagcccca gtcttccggc ctccgtttca
     4741 tcaacaacgc cacggcgtaa tgctgccgct acgcagatat tcagcgccac accatgttgc
     4801 gcattcaact gttgccagcc gcgcacaagg tcaaattcgt cgcttgccgg tgaggtcaag
     4861 tggttagcgt tatagacccc ttcccgatag aaaaagacgc tgcttaactt atggccttct
     4921 gctatcagcg cctgcgcaaa ctgaaaagca ctactcgcct gttgcgtacc gtatgctggc
     4981 ccggtcacca cgatggcaaa acgcattact tatcttgccc ctggaaatca ccgctcttga
     5041 actggcggat gtagagatag acagtgtgtt tggagatgtt caggcggtca gcaacctggt
     5101 tgatcgcatc tttaatatcg aagatccctt tctcgtagag attcagcacg atctgacgat
     5161 ttttggcgtt attagaaaca ttgcgatcgg cattcacttc ttcgatggtg aactccagcg
     5221 tttgggtaac cagatcttca acagaagagg caaagttgac gcttgaaccg acatccgggg
     5281 tttctggcgg cacaaaggtg ctcataatct gcgagaaggg aacatcaaga ttcatattga
     5341 tgcacagcag accaattaca cgctgttcgc ggttacgaat cgcgatagtc agggacttca
     5401 ttaatacgcc gcttttggcg cgagtaaagt agcatttaga aacgctgcta tccgctcccg
     5461 tcatatcgtg cagcatacgt agcgccaggt cagtaattgg cgaaccaatc ttccggcctg
     5521 tatgttcacc gttagcaatg cgaatggctg aacattttag atcctgcaaa gagtgcaaaa
     5581 cgatttcaca gtgggagcca ataagcatcg ctaacccgtc caccaccgct tcgtaggatt
     5641 tcagaatatc aaaatcggtc tggtcgaaag gacgttgatc cagtaaatcc aactcactgg
     5701 tttcgttggt taaaagcgac ctggacatga aaaaaaacac tccttttcag gagcctgtcg
     5761 ttaacttttc agggcaggct cattaatgat gcgggtaact aaattaatac agcggaggtt
     5821 ccgctttcca gcactaatta tatccggcct gtaataaaaa aaccgccgcc tggtcaggcg
     5881 gcggttctta atgcttattt tttagcagaa tctgcggctt tcgcatcagc ttccggcttt
     5941 gcatcagcct tcggcgctgg tttcacatcc agcagctcta cgtcaaacac cagggtagaa
     6001 ttcggtggga tccccggaac acccgctttg ccgtaagcca gttctggtgg aataaccagt
     6061 ttgatcttac cgcctttctt gatgttcttc agaccttctg tccaacccgg gataacaccg
     6121 tccagacgga aagaaagcgg ttcaccacgg gtgtaagagt tgtcgaactc tttaccgtcg
     6181 atcagcgtac ctttgtagtt cactacaaca gtatcgctgt ctttcggtgc ttcgccttta
     6241 ccggcttcta ctacctgata aaccagacca gttgaagagg ttttcacacc tttctctttg
     6301 gcaaatttct cgcggtactc tttacctttt gcttcgttat cagccgcgtc tttttccatc
     6361 ttcgcctgag cagaagactt cacgcgagct tcgaatgctt gcagagtctg ttcgatctct
     6421 tggtcggaga gtttgctctt atcagcaaat gcatcctgaa caccagcgat cagctgatct
     6481 ttatccagtt tgatgcccag tttttcttgt tcttttagag agttttccat gtaacgaccc
     6541 agcgaggcac ccagtgcata agctgatttc tgatcgtcat ttttgaacgc tgctttgctg
     6601 tcagcagttg tagcaggttt tgcagcttca gcagcaaaag tgattggtgc atgcagggca
     6661 acggccattg tggtcgccag cagcgttact ttaaacagtg atttcatcca tatctccagg
     6721 atcggggcat ctcaccccag ggttaactat tatcagaagg gtactataaa gcgttgtcga
     6781 acaaatctac atacagacac gccctattat catctatttt cagactcttt ttgtttaaat
     6841 tagtttcgat gaccgcgaaa tgagtgctgt ctcgggcagc aaagttaagt agaatccgcg
     6901 gcgaccattc gacaaaagag gtgaatcatg caggatttat cattggaagc acgcctggca
     6961 gagctggaga gccgactggc ttttcaggaa atcaccattg aagaactgaa cgtcacggtg
     7021 accgctcatg aaatggagat ggcgaaactg cgcgatcatc tgcgtctgct gaccgagaag
     7081 ttaaaagcca gccagccgtc gaacatcgcg tcgcaggctg aagaaacgcc accgccacat
     7141 tattgaggcg taaaaaaagc ggggattccc cgcttttttg tcactttttc ggtattagtg
     7201 gcaaccgcaa ccgccgttac ctttaccgcc acagcagcct tcgccaccgt gctcatgacc
     7261 gtgatcatgg ccatgaccac cgcagcaacc gtcgtggtcg tgatcgtggt gatgatcgtg
     7321 cgcaccgtga acgtggccat gagccagctc ttcttcagtc gcttcgcgaa tcgccacaac
     7381 ttcaacgttg aatttcaggt tctgaccggc cagcatgtgg ttaccatcaa ccacgacgtg
     7441 atcgtcttca accgcagtga tttcaaccgg taccggaccc tggtcggttt cagccaggaa
     7501 acgcatacct acctgcagtt catcaacgcc cataaatacg tctttaggca cacgttgcac
     7561 caggttttcg tcgtactgac cgtaagcgtc gttcgcgcca acagcgacat caaatttgtc
     7621 gccaacttca tgaccttcca gcgccgtttc caggccagag atcagggaac cgtgaccatg
     7681 caggtagtcc agcggcgcac tcaccggaga ctcatcaacc aacacaccgt cttctgtacg
     7741 tacctgatag gccaggctga ccaccaggtc ttttgctact ttcatgatat ctcctgagca
     7801 tgggaagaat agtggcgcag attgtagcgg aattctgcag ccgtgtactc actagcttaa
     7861 aaaaactcgg cgcatatcgc tagtccggat gaaaaatccc gatcacttgc tcatctttgc
     7921 gaacgtgatc gcgagcttct ttgtctgctt ctcgcatctg atgtccgcac ttaacacatt
     7981 caacaatatc aatattattt tcgcgccaca tcgccattga atcctgcgcc tgacagaccg
     8041 ggcattttgc gcccgcaata aaacgttttc ggattgccat ctttaccctc tactcaaatt
     8101 catcccagcc gtccagctgg cgtcgttctt gttgcatttc acgctggaaa atctcttcca
     8161 gttcgcgtcg ggcttccctg gcgcgagaaa tttgtacggt atcggtatgc attgggatga
     8221 gctctcgcag catccgcata tccaggcggc gaaaatgcag ttgcgcccgc tgtgcctgat
     8281 gcggatgcat accaagcgtg accagcgtct tgcgccccag ctctaacgca ctggagaatg
     8341 tttcacggga aaactgcgtc acccctgcct gtaataactc atgcgcttcc acacgtccgc
     8401 gcgctcgcgc aagaatatgc aaatgcggaa agtgctgctg gcatatttcc accagcttca
     8461 tggtgtcttc cggctcgtta caggtaatga cgatagactc agcggcctct gcacccgcag
     8521 aacgtaaaag atctacctgc gtggcgtcgc cgtaataaac tttgtagcca tatttgcgca
     8581 tcaggttaac ggcgctgata tcccgctcca gcacagtaat gcgcatttta tttgccatca
     8641 acaaacgacc aatcacctgc ccaaaacgtc caaagcccac gacaatgacc tggggtttat
     8701 catcgttgac ccacggtttt tcgtcttctt cttccggtcc gttaaactgg cgggatagcc
     8761 atttatccac cagcttcatc agcagcggcg tggtcatcat ggaaagcgtc accgtcacca
     8821 gcaacagcgc catctggtcg ccctggaata agcgttgtga agaagcggta gaaaagagga
     8881 caaaggcaaa ctcaccgccc tgactcaaca cgccagcaaa ctgcatccgc tctgagctac
     8941 gcacgccata caatcgcgcc agcagataca gcacgagaat tttcaccgcc accagcacaa
     9001 ccacgcttat cactacccac aacagatggg tataaagcac cccgaggttg agtgacatcc
     9061 cgacagagat aaagaacaaa ccgagcagca agcctttaaa gggatcgata gccgtttcca
     9121 gttcatggcg atattcactt tccgccagca gcacgcctgc aataaacgta ccgagcgcca
     9181 tcgacagccc caacgcatcc ataaacaatg cggaacccaa taccagcagc agcgtcgcgg
     9241 cggtgaacac ttcccgcacg ccagaagctg caataaaacg gaataccgga cgcaataaat
     9301 agcgcccgcc aatcagcatg ccgacaaacg ccagcacctt catgccgatc ttcatccagt
     9361 cgaaatgttc gtctgccgaa cccgccaaca acggcactaa cgccagtgcc gggatcaccg
     9421 ccagatcctg aaacagcagt accgaaaacc cgagctgccc ggattcgctg cgattcatcc
     9481 ctttctcacg catcaattgc aacgccattg cggtcgaaga catcgcaagg ccaatgccac
     9541 cgaccaccgc cgcctgccag gcgaaatccg tcagcatcaa taatcccgcc agcaacgccg
     9601 cgcttaacag cacctgtgcc gcgcctacgc caaaaatcga acgccgcagt tgccaaagct
     9661 tggagggatt caactcaagg ccgatgataa acatcaggaa caccacgccg agttccgaaa
     9721 agtggaggat ctcatcgacg tcgctaataa accccagccc ccacgggcca attgcaatcc
     9781 ctgccagcaa atatcccaac acagcgccaa tacccagccg cgatgccaac ggcaccgcag
     9841 ccaccgccgc gaagagaaac agcactcctg caagtaaaaa atcggaacct tccatcagcg
     9901 gcctcctgga gacagcggat ttgccagcca gtcaccgtag gctctggcgt ggctcgccag
     9961 ctcctgtgcg ctttgccgtc tcgcccagta aataatgatg ggacttaacc aatgcatccg
    10021 gcacatgccc gccgccagtt caaaggggcg caggacatcg ctcatcgggt agcgattcaa
    10081 cgcgtcataa cggtaagcac tttccggctc gccggtggta atcacactac gccagtactt
    10141 tcccgccagt tggtttcctc ccggcccgct ggcaaaacca cggcttaata cccggtccag
    10201 ccactctttc agtagcgccg ggcagctata ggtataaaga ggatgctgaa agacaatcac
    10261 ctcgtgctcg cgcagtaatg cctgctcacg ggggatatca ataaaaaaat cgggatagtg
    10321 cgcgtaaagg tcgtgcacgg taacattgct gagctgcgtg gccggtttaa gcagtacccg
    10381 gtttgccacc gagtcctgag attccggatg ggcatacagc agcaaaactt tcgctggctg
    10441 agacatcatc cccctcccgg tatggttttt gtgtatagtc gctgttttgg gctaccattg
    10501 cgcccggtgc ggcagctcgc ccatacatta cattatcata atgataagtt aacatagtct
    10561 gaacatacgg caccttatga ttgttttctc ctcgttacaa attcgtcgcg gcgtgcgcgt
    10621 cctgctggat aatgccaccg ccaccatcaa ccccgggcag aaagtcggcc tggtgggtaa
    10681 aaacggctgt ggtaaatcta ccctgctggc attgctgaaa aatgaaatca gcgccgacgg
    10741 cggcagctac acctttccgg gaagctggca actggcgtgg gtgaatcagg aaacgccggc
    10801 gttaccgcaa gcggcgctgg aatatgtcat tgacggcgac cgtgaatatc gtcaactgga
    10861 agcgcagcta cacgacgcca acgaacgtaa cgacgggcac gccattgcga ccattcatgg
    10921 caagctggat gctattgacg catggagtat tcgctcccgt gccgccagcc tgctgcacgg
    10981 cctcggtttc agcaatgaac aactggagcg cccggtaagt gatttctctg gtggctggcg
    11041 tatgcgtctt aaccttgccc aggcgctgat ttgccgttca gacttgctgc tgctcgacga
    11101 accgactaac cacctcgatc tcgatgccgt tatctggctg gaaaaatggc tgaagagcta
    11161 tcagggcacg ctgatcctga tctctcacga ccgcgacttc ctcgatccga tcgtcgataa
    11221 aattattcat atcgaacaac aaagcatgtt cgagtacacc ggcaactaca gttcgtttga
    11281 agtacagcgc gccacccgtc tggcgcagca acaagcgatg tacgaaagcc agcaggaacg
    11341 cgtagcgcat ctgcaaagtt atatcgaccg tttccgtgcc aaagccacca aagcgaagca
    11401 ggcccagagc cgcattaaga tgctcgagcg tatggagctg attgcccccg cgcacgtcga
    11461 caacccgttc cgctttagct tccgcgcgcc ggaaagcctg ccaaatccgt tactgaagat
    11521 ggaaaaggtc agcgcaggct atggcgatcg cattattctc gactcgatta aactgaatct
    11581 ggtccccggt tcgcgcattg gtctgttagg ccgcaacggc gcgggtaaat cgacattaat
    11641 caaactgtta gccggtgaac ttgcgccagt cagtggtgaa ataggcctgg caaaagggat
    11701 caagctcggc tacttcgccc agcatcaact tgaatacctg cgcgccgacg aatcgcctat
    11761 tcaacatctg gcacgtttag cgccgcagga actggagcaa aaactgcgtg actacctcgg
    11821 cggctttggt ttccagggcg ataaagtaac cgaagaaacg cgccgcttct ccggtgggga
    11881 aaaagcccgc ctggtgctgg cattaattgt ctggcagcgg ccgaatctgc tgctgctcga
    11941 cgaaccgact aaccaccttg acctcgacat gcgtcaggca ctcaccgaag cattaatcga
    12001 gtttgaaggc gcgctggttg tcgtttcgca cgaccgtcat ttgctgcgtt ccaccactga
    12061 cgacctctac ctggttcacg atcgtaaagt cgagccgttc gacggcgatc tggaagatta
    12121 tcaacagtgg ttgagcgacg tacaaaagca ggaaaaccag accgacgacg cgccaaaaga
    12181 gaacgcgaac agcgcccagg cacgtaaaga tcagaagcgt cgggaagcgg agttgcgtgc
    12241 gcaaacccag ccactgcgta aagagattgc ccgtctggaa aaagagatgg agaagctgaa
    12301 cgcgcaactg gcgcaggcag aagagaaact cggcgacagc gaactgtatg accagagccg
    12361 taaagcggag ttgaccgcct gcctgcaaca gcaagccagc gccaaatccg gcctggaaga
    12421 gtgcgaaatg gcatggctgg aagcccagga gcagcttgag cagatgttgc tggaaggcca
    12481 aagcaactga tggcgcagat aacgacgacc gatgccaatg aattcagcag cagtgctgaa
    12541 ttcaccccta tgcgcggctt tagcaattgt catctgcaaa ccatgctgcc gcgtctgttt
    12601 cgtcgcaagg tgaaattcac cccgtactgg cagcggctgg agttgcccga cggcgatttt
    12661 gtcgatctcg cgtggagtga agccccagca caggcgcggc ataaaccgcg tttagtggta
    12721 tttcacgggc tggaaggcag tctcaatagc ccttacgccc acggtctggt tgaggcggcg
    12781 caaaagcgcg gctggctggg cgtggtgatg cattttcgcg gatgcagcgg tgaaccaaac
    12841 cgtatgcacc gcatttacca ttcgggcgaa accgaagacg ccagttggtt tttacgctgg
    12901 ctgcaacgcg aatttggtca tgcgccaacg gctgccgtcg gctattcgct cggcggtaat
    12961 atgctggcct gtttgctggc aaaagaaggc aatgatctcc ctgttgatgc ggcggtgatt
    13021 gtctctgcgc cgtttatgct ggaagcctgt agctatcata tggaaaaggg cttttcccgc
    13081 gtttatcagc gttacttgct gaacctgtta aaagccaatg ccgcgcgcaa gctggcagcc
    13141 taccccggaa cactgccgat taatctcgcg cagttaaaat cggtacgtcg catccgtgaa
    13201 tttgacgatc tcatcaccgc cagaattcac ggctacgctg acgctatcga ctattatcgt
    13261 cagtgtagcg ccatgccgat gctgaaccgg atcgccaaac cgacgctgat tattcacgcc
    13321 aaagacgatc cgtttatgga tcatcaggtg atcccgaaac cggaaagtct ccccccgcag
    13381 gtggagtatc aactgactga acatggcggt catgttggct ttattggcgg tacattactt
    13441 catccgcaaa tgtggctgga gtcacgcatt cctgactggt taacaacgta tctggaggcg
    13501 aaatcatgtt gattccgtgg caagacctct cccccgaaac gctggaaaat ttaattgaaa
    13561 gctttgtgtt acgtgaaggc accgattatg gtgaacatga gcgtacactt gaacagaaag
    13621 tcgccgacgt caaacgccag ctacagtgcg gagaagcggt gctggtatgg tcggagctgc
    13681 acgaaacggt caatattatg ccgcgcagtc agtttcgcga ataacctgca accgtcggcg
    13741 gacgagataa taatgatagc taagtcatgg agttactatg tctgccaaac atccggtcat
    13801 tgcggtaaca ggatccagcg gcgcggggac caccaccacc agcctcgcgt ttcgtaaaat
    13861 attcgcgcag ttaaatctgc atgcagccga ggtggaaggc gacagttttc accgttacac
    13921 ccgcccggaa atggacatgg cgatccgcaa agcgcgcgac gccgggcggc atatcagcta
    13981 cttcggcccc gaagcgaacg acttcggcct gctggaacaa accttcattg aatacggtca
    14041 gagcggcaaa gggaaatctc gcaaatatct gcatacctac gatgaagccg taccgtggaa
    14101 tcaggtaccg ggaacattca ccccctggca acctttacca gaacccactg atgtactgtt
    14161 ttatgaaggt ttacacggcg gcgtagtcac gccacagcat aacgttgcgc agcatgtgga
    14221 cttactggtc ggcgtggtgc ctatcgttaa ccttgagtgg attcaaaaac tgatccgcga
    14281 caccagcgag cgcgggcact cacgagaagc agtgatggac tcagtagtgc gttcaatgga
    14341 agactatatc aactacatca caccgcagtt ttcccgcacc catcttaact tccagcgcgt
    14401 tcccaccgtc gacacttcaa acccgttcgc ggcaaaaggt atcccgtcgc tggatgaaag
    14461 ctttgtggtg atccattttc gtaatctgga agggatcgat ttcccctggc tgctggcgat
    14521 gttgcaaggc tcattcattt cccacatcaa tacgttagtg gtacctggcg gcaaaatggg
    14581 gctggcaatg gaattaatta tgctgccgct ggtgcaacga ttgatggaag gaaagaaaat
    14641 cgagtaactt tgctattatg ccggataaaa tactatccgg cttcacaacg ggatagttaa
    14701 atcacgcggc aaccacttca tacgagtgag taatattcac cgctttttcc agcatcaacg
    14761 ccactgaaca atatttctca gcagagagat caaccgcacg cgcaaccgct gcgtctttca
    14821 ggtcgcgacc ggtgacgata aaatgcagat taatgtgcgt aaacaggcgt ggtgcctctt
    14881 cgcggcgttc agaggtcaat tttacttcac aatcgaccac atcctgacgc cctttttgca
    14941 ggatagatac cacatcgatg gcactgcaac cacccgccgc catcaacacc atttccatcg
    15001 gacttggtgc tttatcgcct gagttgccgt ccattaaaat ctgatgacca gaggcggatt
    15061 cgcccagaaa agttaaccct tcgacccact tcactcgcgc ttgcattttt gctactccac
    15121 tgcgtcaatt ttcctgacag agtacgcgta ctaaccaaat cgcgcaacgg aaggcgacct
    15181 gggtcatgct gaagcgagac accaggagac acaaagcgaa agctatgcta aaacagtcag
    15241 gatgctacag taatacattg atgtactgca tgtatgcaaa ggacgtcaca ttaccgtgca
    15301 gtacagttga tagccccttc ccaggtagcg ggaagcatat ttcggcaatc cagagacagc
    15361 ggcgttatct ggctctggag aaagcttata acagaggata accgcgcatg gtgcttggca
    15421 aaccgcaaac agacccgact ctcgaatggt tcttgtctca ttgccacatt cataagtacc
    15481 catccaagag cacgcttatt caccagggtg aaaaagcgga aacgctgtac tacatcgtta
    15541 aaggctctgt ggcagtgctg atcaaagacg aagagggtaa agaaatgatc ctctcctatc
    15601 tgaatcaggg tgattttatt ggcgaactgg gcctgtttga agagggccag gaacgtagcg
    15661 catgggtacg tgcgaaaacc gcctgtgaag tggctgaaat ttcgtacaaa aaatttcgcc
    15721 aattgattca ggtaaacccg gacattctga tgcgtctgtc tgcacagatg gcgcgtcgtc
    15781 tgcaagtcac ttcagagaaa gtgggcaacc tggcgttcct cgacgtgacg ggccgcattg
    15841 cacagactct gcttaatctg gcaaaacaac cagacgctat gactcacccg gacggtatgc
    15901 aaatcaaaat tacccgtcag gaaatcggtc agattgtcgg ctgttctcgt gaaaccgtgg
    15961 gacgcattct gaagatgctt gaagatcaga acctgatctc cgcacacggt aaaaccatcg
    16021 tcgtttacgg cactcgttaa tcccgtcgga gtggcgcgtt acctggtagc gcgccatttt
    16081 gtttcccccg atgtggcgca gactgattta tcaccccgat attaactatg cactacgaca
    16141 aacgctggtg ctatgtttgc ccgtggccgt tgggttaatg cttggcgaat tacgattcgg
    16201 tctgctcttc tctctcgttc ctgcctgttg caatattgcg ggccttgata cgcctcataa
    16261 acgttttttc aaacgcttaa tcattggtgc gtcgctgttt gccacctgta gcttactgac
    16321 acagctgcta ctggcaaaag atgttcccct gccctttttg ctgaccggat taacgctggt
    16381 acttggcgtc accgctgagt tggggccatt gcacgcaaaa ttgcttcccg catcgctgct
    16441 ggcagccatt tttaccctca gtctggcggg atacatgccg gtctgggaac cgttgctcat
    16501 ctatgcgttg ggcactctct ggtacggatt gtttaactgg ttttggttct ggatctggcg
    16561 cgaacaaccg ctgcgcgagt cactaagtct gctgtaccgt gaactggcag attattgtga
    16621 agccaaatac agcctgctta cccagcacac cgcccctgaa aaagcgctgc cgccgctgct
    16681 ggtgcgccag caaaaagcgg tcgatctaat tacccagtgc tatcagcaaa tgcatatgct
    16741 ttccgcgcaa aataatactg actacaagcg gatgctgcgt attttccagg aggcgctgga
    16801 tttgcaggaa catatttcgg tcagtttgca tcagccggaa gaggtgcaaa aactggtcga
    16861 gcgtagccat gcggaagaag ttatccgctg gaatgcgcaa accgtcgccg ctcgcctgcg
    16921 cgtgctggct gatgacattc tttaccatcg cctgccaacg cgttttacga tggaaaagca
    16981 aattggcgca ctggaaaaaa tcgcccgcca gcatccggat aatccggttg ggcaattctg
    17041 ctattggcac ttcagccgca ttgcccgcgt gctgcgcacc caaaaaccgc tctatgcccg
    17101 tgacttactg gccgataaac agcgacgaat gccgttactt ccggcgctga aaagttatct
    17161 gtcactaaag tctccggcgc tacgcaatgc cggacgactc agtgtgatgt taagcgttgc
    17221 cagcttgatg ggcaccgcgc tgcatctgcc gaagtcgtac tggatcctga tgacggtatt
    17281 gctggtgacg caaaatggct atggcgcaac ccgtctgagg attgtgaatc gctccgtggg
    17341 aaccgtggtc gggttaatca ttgcgggcgt ggcgctgcac tttaaaattc ccgaaggtta
    17401 caccctgacc ttgatgctga ttaccaccct tgccagctac ctgatattgc gcaaaaacta
    17461 cggctgggcg acggtcggtt ttactattac cgcagtgtat accctgcaac tgttgtggct
    17521 gaacggtgag cagtacatcc ttccgcgtct tatcgatacc attattggtt gtttaattgc
    17581 tttcggtggt accgtctggc tgtggccgca gtggcagagc gggttattgc gtaaaaacgc
    17641 ccatgacgct ttagaagcct atcaggaagc gattcgcttg attctgagcg aggatccgca
    17701 accaacgcca ctggcctggc agcgaatgcg ggtaaatcag gcgcataaca ctctgtataa
    17761 ctcattgaat caggcgatgc aggaaccggc attcaacagc cattatctgg cagatatgaa
    17821 actgtgggta acgcacagcc agtttattgt tgagcatatt aatgctatga ccacgctggc
    17881 acgggaacac cgggcattgc cacctgaact ggcgcaagaa tatttgcagt cttgtgaaat
    17941 cgccattcag cgttgtcagc agcggctgga gtatgacgaa ccggggagtt ctggcgatgc
    18001 caatatcatg gatgcgccgg agatgcagcc gcacgaaggc gcggcaggta cgctggagca
    18061 gcatttacag cggattattg gtcatctgaa caccatgcac acaatttcgt cgatggcatg
    18121 gcgtcagcga ccgcatcacg ggatttggct gagtcgcaag ttgcgggatt cgaagacgta
    18181 atacaggcta aaccatcgga tgaggtgttc acgccgcatc cgacatcttt ttcctgatgc
    18241 gtcttattta cgccccaaca actttcgcca ccgcgtgggc gaaacgttgc atcccttcat
    18301 cgatatccgc atcttccacc accagcgacg gtgcaaaacg catcacatcc ggtccggcat
    18361 tcagcaccat tacgccagcc tctgcgcccg catacaggaa atcacgcgcc cgacctttgt
    18421 actgtggctt aagctccgcg ccaatcaaca gccccatacc gcgaatatcg ctaaacacat
    18481 cgtactgctg atcgatcttc tgcaaatggt caacaaaacg ctggcgtttc gcctgaatgc
    18541 cttccagcac ttcaggtgta ttgatgatat caaacgccgc ccccgctact gcacaggcca
    18601 gaggattacc gccgtaggtg gaaccgtgag aaccaggatg aaacgcagaa gcaatttccg
    18661 ccgtggtcag catggcgcta atcgggaagc cgccgcctaa cgctttcgca gaagtcagaa
    18721 tatccggcgt cacgccgtag tgcatgtaag caaacaaatc gccagtccgc cccatcccgc
    18781 actgcacttc atcaaacacc aataatgcct gatattgatc gcacagctcg cgcaagccct
    18841 gcaaaaactc tggcgtcgct gccgtcacac cgccctcgcc ctggatcggc tcaaccacca
    18901 ccgcacaggt gtgatcatcc atcaccgctt tcactgcgtg gagatcgtta aagggaacgt
    18961 ggatgatgtc tgctggtttt ggcccaaagc cgtcggaata ttttggctgc ccacccaccg
    19021 aaacggtaaa cagcgagcga ccatgaaaag cgttatggaa ggcaataatt ttggttttga
    19081 acgggctatg acgcacacag gcgtaatggc gtgccagttt aaaggcggtt tcgttagctt
    19141 ccgtgccaga gttcataaac accacacgtt cggcaaacgt tgcctcaatc agtttacgcc
    19201 caagacgcag cgccggttca ttggtgaaaa cgttactgat atgccacaga gtttcgccct
    19261 gggtttttaa cgcgttcacc agcgcaggat ggcaatggcc caacgccgta actgcaatgc
    19321 cacccgcgaa atcgacatac tccttgcctt gctgatccca gattcggctg ccctgacctt
    19381 ttaccggaat gaactctgcc ggagcataaa tcggcaggat cacttcatcg aaagtcgcgc
    19441 gtgtaattgc tgtttgttca attgccatct catgatcacc ctgttacgca taaataaatg
    19501 tgaaattata atcacaaaat atgcataaaa aatcattgaa tggcaatcag aaatcagcga
    19561 tgtaggaaat tagccagcag ttgatgtcct tgttcgctaa gaatactttc tggatggaac
    19621 tgcacacctt ccagatccca ctggcgatgg cgaatcccca taatctcgcg ggtttcgctc
    19681 caggccgtca cgtcaaagca cgcaggtaat gagtcaggtt ccaccaccag cgaatgatag
    19741 cgtgtcacag taagtggatt tgccagcccc cgaaatacgc cctcaccgct atgtgtaatc
    19801 ggcgaggttt tgccgtgcat gacctttgca gcgcgcacaa ctttaccgcc aaatgcctgc
    19861 gccattgcct gatgaccgag gcagacgcca agaatcggca agcgcccggc atagtggcga
    19921 ataacgtcaa gggagatccc ggcttcatct ggcgtacagg ggccgggtga gatgacaatt
    19981 ttttgtggtt taagggcgtc gatatccgcc agcgtcaacg catcgttgcg cttaaccagc
    20041 acatccgccc ccagttcaca aaaatactgg tagaggttcc aggtaaaaga atcgtagtta
    20101 tctataagca ggatcatggc ggctccggta cgaaagaacc gcgctatttt actcagattc
    20161 cccggcttcg cttactactt tgctaaatat catctgcagt gcggtgagat cccccattgc
    20221 cccactctga tttgcctgat tccaggcctc tttttcaata ccctgccagc tcagttgata
    20281 tccggcatga atcgccagtt gctcgaagaa gatccgctgt gccagaccac ttcccacccg
    20341 gaagggatgc agcacgttga tttcacaata gtaatgcgcc agccgctcga cgaacttcgc
    20401 tttctccagg ccaaccagat aaccttcttc ctccagatcc tgcatcaggg cattgccctc
    20461 tttttcgata taagcaaagt ggcagaacgg cgtatcgccc tgataaatat caacttcacg
    20521 cagttgccct gcccagtcga aaatatcctg atacagctgg cgatggatag tgcgcaaatg
    20581 cggtaaaccg cgcaccagcg gaccaagctc aatggtcgca gcacgcagcg ccgtcatttc
    20641 gtaagcggcc tgttccagcc gctgctgctg gcggatgttc agccggttac gcatgatatc
    20701 aaggcctgga taaagatacg gatcgcgccc ttcgccgaat ttatcgctca tagtgcctcc
    20761 tcagctcttc aaggcgcgct aaagcctctg cggcagtaag agtgactaaa ggcatctcga
    20821 cgccttcaag acggcgactg gcctggaaat tacgattacg ctgaagctcc cagagacggg
    20881 acttttgctt atcggtgagt ttcttcactt gatgcctccc tgaacgtgtc catttgccac
    20941 aagtataagc ggcaaatccg ggttacgccg ggagagcaga agcgcgggca agattgcccg
    21001 cgcgagaaat cattacggca ggactttagc ggaaaggata actaccggtt ttgacggcac
    21061 attctggtac ggaccaacgt catgagtcgg cacctgggaa atcttatcgg caacgtccat
    21121 gcctttcacc actttaccaa ataccgcgta accgaaatca cgctgaccat ggtcaaggaa
    21181 ggcgttatcg gcaacgttga taaagaactg gctggtggcg ctgtctttgt cggcggtacg
    21241 cgccatcgcg atggtaccac gcgtgttgcg caggccgtta tcggcttcat ttttgattgg
    21301 cgggtttggt tttttctgct gcatctgctc ggtgaaaccg ccgccctgaa tcataaagcc
    21361 aggaatgacg cggtgaaagg tagtattgtt ataaaaaccg ctgttcacat aatcgacaaa
    21421 gttctgcaca gacactggcg ctttttgttt atccagctcc agttcgatgt taccagctga
    21481 ggttgtcaac aatacgtgcg ggtccccttt cgctgccatt gctgcgggag aaagagcaga
    21541 aagagcgaaa acagcagcca tcgccgccag ggtcgatttg aacatgagat ttccttaaca
    21601 aacagagcag aaaaaaagca agtgaagaga ttctaaatag ccttcaagaa caaggccatc
    21661 cctttaccta attttacgta tctgaaatat atgtaacctg acgatcactt tttattccgt
    21721 gatcaaaatc acctcttaaa atgcaattta gcaaccgatt gcaataaaac atttaaacag
    21781 atcacaaaat cacctaaaat cgcccgctcg cggcatatcc cgctatgcca ttttattttt
    21841 gaacgcaggc caatcatgac taacagcaat cgcatcaagc tcacatggat tagctttctc
    21901 tcctacgcac tgactggtgc gttggttatt gtcaccggga tggtgatggg aaatatcgcc
    21961 gattatttca atctgcctgt ttccagtatg agtaacacct tcaccttcct caacgccggc
    22021 attttaatct ctatattcct caacgcctgg ctgatggaaa tcgtcccgtt gaaaacgcag
    22081 ttacgttttg gctttctcct gatggtgctg gcggttgccg gtttgatgtt cagccacagc
    22141 ctggcgctgt tctcggcggc gatgttcatt ctcggggtgg tcagcggcat caccatgtcg
    22201 attggtacat tcctggtaac acaaatgtat gaagggcgtc agcgcggttc ccgcctgtta
    22261 tttaccgact ccttcttcag tatggctggg atgattttcc caatgatcgc cgcgtttcta
    22321 ctggcgcgca gcattgagtg gtactgggtt tatgcctgca tcgggctggt gtatgtcgct
    22381 atttttattc tgactttcgg ctgtgagttc ccggcgctgg gcaaacatgc gccaaaaacg
    22441 gatgctccgg tagcgaaaga aaagtggggg atcggcgtac tgtttctctc tgttgcggcg
    22501 ctgtgctaca tcctcggtca gttaggtttt atctcctggg tgcctgagta tgccaaaggc
    22561 ctgggcatga gcctgaacga cgcgggcacg ctggtgagta acttctggat gtcatacatg
    22621 gtcggcatgt gggcgttcag ctttattctt cgcttctttg atttgcaacg cattctgacc
    22681 gtactggctg gtctggctgc gattctgatg tacgtcttta acaccggaac accggcacat
    22741 atggcgtggt caattctcgc cctgggcttc ttctccagcg cgatctatac caccatcatc
    22801 accctgggtt cacagcagac caaagtaccg tcgccaaaac tggttaactt tgtcctgacc
    22861 tgcgggacca tcggtactat gttgaccttt gtggttaccg gcccgattgt tgaacatagc
    22921 ggtccgcagg cggcactgct tacagcaaac ggtctgtacg ctgtcgtctt tgtaatgtgc
    22981 ttcctgttag gttttgtcag ccgtcaccgt cagcataaca ccctgacctc tcattaattg
    23041 ctcatgccgg acggcactat cgtcgtccgg ccttttcctc tcttcccccg ctacgtgcat
    23101 ctatttctat aaacccgttc attttgtcca ttttttgcac aaacatgaaa tgtcagacaa
    23161 ttccgtgact taagaaaatt tatacaaatc agcaatatac cccttaagga gtatataaag
    23221 gtgaatttga tttacatcaa taagcggggt tgctgaatcg ttaaggtagg cggtaataga
    23281 aaagaaatcg aggcaaaaat gagcaaagtc agactcgcaa ttatcggtaa cggtatggtc
    23341 ggccatcgct ttatcgaaga tcttcttgat aaatctgatg cggccaactt tgatattacc
    23401 gttttctgtg aagaaccgcg catcgcttat gaccgcgtac acctctcgtc ttacttctct
    23461 caccacaccg ccgaagagct gtcgctggtg cgcgaaggct tctacgagaa acacggcatc
    23521 aaagttctga tcggcgaacg cgctatcacc atcaaccgtc aggagaaggt gattcactcc
    23581 agcgccgggc gcaccgtttt ttatgacaag ctgatcatgg cgaccggttc ctacccgtgg
    23641 atcccgccaa tcaaaggttc tgatactcag gactgctttg tctatcgtac tattgaagac
    23701 ctcaacgcca ttgaatcctg cgcccgtcgc agcaaacgcg gtgccgttgt tggtggcggc
    23761 ctgttaggtc tggaagccgc tggcgcgctg aaaaacttag gtattgaaac ccacgttatc
    23821 gaatttgccc ctatgctgat ggcggaacag cttgatcaga tgggtggcga gcagctgcgt
    23881 cgcaaaatcg aaagtatggg cgtgcgcgtt cacaccagca aaaacaccct tgagattgtg
    23941 caggaaggtg tcgaagcgcg taaaaccatg cgttttgccg acggcagcga actggaagtc
    24001 gactttatcg tcttctctac cggtatccgt ccgcgcgata agctggcaac ccagtgtggt
    24061 ctggacgttg ctccgcgtgg gggtattgtt attaatgatt cctgccagac ttccgatccg
    24121 gatatctacg ccatcggtga atgcgcaagc tggaacaacc gtgtatttgg tctggtagca
    24181 ccaggctaca aaatggcgca ggtcgccgtt gaccatattc tcggtagcga aaacgccttt
    24241 gaaggtgctg accttagcgc caagctgaaa ctgctgggcg tagacgtagg cggtattggt
    24301 gatgcgcacg gtcgcacacc aggcgcacgt agctacgttt acctcgacga aagcaaagag
    24361 atctacaaac gccttattgt cagcgaagac aacaaaaccc tgctcggtgc ggtactggtg
    24421 ggcgatacca gcgactacgg caacctgctg caactggtgc tgaatgctat cgagctgccg
    24481 gaaaacccgg attctctgat tctgccggcg cactcgggta gcggtaagcc gtctatcggt
    24541 gttgataaac tgccggacag cgcgcaaatc tgctcctgct tcgacgtcac caaaggtgat
    24601 ctgattgctg ccatcaacaa aggctgtcac accgttgcgg cgctgaaagc cgaaaccaaa
    24661 gcgggtactg gctgcggtgg ctgtatcccg ctggtcactc aggtactgaa cgcggaactg
    24721 gcgaaacagg gcatcgaagt taacaacaac ctgtgcgaac actttgctta ttcgcgtcag
    24781 gaactgttcc atttgatccg cgttgaaggc attaaaacct tcgaagaact gctggcgaaa
    24841 cacggcaaag gttacggttg tgaagtttgt aaaccgaccg tcggttcgct gctggcctcc
    24901 tgctggaacg aatacattct gaagccggaa catactccgc tgcaggattc taacgacaac
    24961 ttcctcgcta acatccagaa agacggcacc tactcggtga tcccgcgctc tccgggcggt
    25021 gaaatcaccc cggaagggct gatggcagtg ggtcgtatcg cgcgtgaatt taatctctac
    25081 accaaaatca ccggctccca gcgcctggcg atgtttggcg cacagaaaga cgatctgccg
    25141 gaaatctggc gtcagctgat tgaagccggt ttcgaaaccg gtcatgccta tgcgaaagca
    25201 ctgcgtatgg cgaaaacctg cgtgggtagc acctggtgcc gctacggcgt gggcgacagc
    25261 gtgggcctcg gcgtggaact ggaaaaccgc tacaaaggca tccgtacgcc gcacaaaatg
    25321 aagttcggtg tctccggctg tacccgtgaa tgttcagaag ctcagggtaa agacgtgggt
    25381 attatcgcca ctgaaaaagg ctggaacctg tatgtttgcg gtaacggcgg catgaaaccg
    25441 cgtcatgcgg atctgctggc ggcggatatc gatcgcgaaa cgctgatcaa atatctcgac
    25501 cgcttcatga tgttctacat ccgtaccgcc gacaaactga cgcgtaccgc gccgtggtta
    25561 gaaaacctcg aaggcggcat cgattacctg aaagcagtga tcattgacga caaactgggg
    25621 ctgaacgcac atctggaaga agagatggcg cgcctgcgtg aagcggtagt gtgtgagtgg
    25681 actgaaacgg tcaatacgcc gtctgcgcag actcgcttca aacacttcat caacagcgac
    25741 aaacgtgacc cgaacgtaca gatggtgcca gagcgcgaac agcaccgtcc ggcaacgccg
    25801 tatgaacgta tcccggtaac tctggtggag gacaacgcat gagccagtgg aaagacatct
    25861 gcaaaatcga tgacatcctg cctgaaaccg gcgtctgcgc gctgttaggt gacgagcagg
    25921 tcgcgatttt ccgcccgtat cacagcgatc aggtgtttgc gatcagcaac atcgacccgt
    25981 tcttcgagtc cagcgtgcta tcacgcggac tgattgcgga acaccagggc gagctgtggg
    26041 tcgccagccc gctgaaaaaa cagcgttttc gcttaagcga cggcttgtgc atggaagacg
    26101 aacagttttc cgtcaaacat tacgaagcgc gagtgaaaga cggcgtggtg cagctgcgcg
    26161 gttaatgttt taacgggagg cgcaatgcct cccctttttg catggtcctg taataatctt
    26221 cggtatattg caggacattt tttaaacttt ttgttttatt ttttgttttt attttttaaa
    26281 ggataatcaa atgtttacag acactattaa taagtgtgcg gctaacgctg cgcgcattgc
    26341 acgcctgtcg gcaaataacc cgctcggctt ttgggtcagc tccgccatgg cgggcgcgta
    26401 tgtgggtctt gggatcatcc tgatttttac gctcggtaat ttgctcgatc catccgtacg
    26461 ccctctggtg atgggcgcga cctttggtat cgccttaacg ctggtgatta tcgccggttc
    26521 tgaactgttc accggacaca ccatgttcct cacctttggg gtgaaagcgg gcaccatcag
    26581 ccacgggcaa atgtgggcaa tcctgccgca aacctggctg ggtaacctgg tcggttccgt
    26641 cttcgttgcc atgctttata gctggggggg cggtagcctg ctgccggtag ataccagcat
    26701 cgttcactcc gtcgcgctgg ctaaaaccac tgcaccggca atggtactgt tcttcaaagg
    26761 tgcattgtgt aactggctgg tttgtctggc aatctggatg gcgctgcgca ctgaaggggc
    26821 ggcgaaattt atcgcaatct ggtggtgtct gctggcattt atcgcgtccg gctacgagca
    26881 ctccatcgct aacatgacgc tgttcgcgct ctcctggttc ggcaaccaca gcgaagccta
    26941 cacgctggcg ggtattggtc ataacctgct gtgggtgacg ctgggtaata ctttatcagg
    27001 tgccgtattc atgggattgg gttattggta tgctacgccg aaagcgaatc gtccggttgc
    27061 ggacaaattt aatcaaactg aaacggctgc cggttaatta ctaaggggtt tttacgtgga
    27121 tcatttgcct atattttgcc aattacgcga tcgcgactgt ctgattgtcg gcggtggtga
    27181 tgtcgcggaa cgcaaagcaa ggttgctgtt agacgcaggc gctcgcttaa cggtgaatgc
    27241 attagcgttt attccacagt tcaccgcatg ggcagatgca ggcatgttaa ccctcgtcga
    27301 agggccattt gatgaaagcc ttctcgacac ctgctggctg gcgattgcag cgacggatga
    27361 tgacgcgctt aaccagcgcg tcagcgaagc cgctgaagct cgtcgcatct tctgtaacgt
    27421 ggtcgatgcg ccgaaagctg ccagcttcat tatgccgtcg attattgacc gctcaccgct
    27481 gatggtggcg gtttcctctg gcggcacctc tccggttctg gcacgcctgt tgcgcgaaaa
    27541 acttgaatca ctgctgccgt tacatctggg ccaggtagcg aaatacgccg ggcaattacg
    27601 cggtcgagtg aaacaacagt tcgccacgat gggtgagcgt cgccgtttct gggagaaatt
    27661 gtttgttaac gaccgcctgg cgcagtcgct ggcaaacaac gatcagaaag ccattactga
    27721 aacgaccgaa cagttaatca atgaaccgct cgaccatcgc ggtgaagtgg tgctggttgg
    27781 tgcaggtccg ggcgatgccg ggctgctgac actgaaagga ctgcaacaaa ttcagcaggc
    27841 agatgtggtg gtctacgacc gtctggtttc tgacgatatt atgaatctgg tacgccgcga
    27901 tgctgatcgc gttttcgtcg gcaaacgcgc gggataccac tgcgtaccgc aggaagagat
    27961 taaccagatc ctgctgcggg aagcgcaaaa aggcaaacgc gtggtgcgtc tgaaaggtgg
    28021 cgatccgttt atttttggcc gtggtggcga agagctggaa acactgtgca acgcgggtat
    28081 tccgttctcg gtggttccgg gtattaccgc agcttctggt tgctctgcct attcaggtat
    28141 tccgctcacg catcgcgatt atgcccagag cgtacgctta attaccggac acttaaaaac
    28201 cggtggcgag ctggactggg aaaacctggc ggcagaaaaa cagacgctgg tgttttatat
    28261 ggggctgaat caggctgcga ctattcagca aaagctgatt gaacacggta tgcctggcga
    28321 aatgccggta gcaattgtcg aaaacggaac agcagtcacg cagcgcgtga ttgacggtac
    28381 gctcacacaa ctgggcgaac tggcgcagca aatgaacagt ccatcgctaa ttattattgg
    28441 tcgggttgtt ggcctgcgcg ataaactgaa ctggttctcc aaccattaat ttaatcaggg
    28501 ccagagaatt ctggcctttt tcgtttacaa ctctctttat atattcagca gataaaaaca
    28561 taacccctta ataaatatta tctgctaatg ggttatattc accgttacag cgacttacaa
    28621 aattaagtca ttcactcact gacgccgatt aaatattcct gtatcgt
//
LOCUS       DAZKFU020000035        28400 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZKFU020000035 DAZKFU020000000
VERSION     DAZKFU020000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.035"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..696)
                     /locus_tag="ACHOQQ_RS06110"
                     /old_locus_tag="pgaptmp_001222"
     CDS             complement(<1..696)
                     /locus_tag="ACHOQQ_RS06110"
                     /old_locus_tag="pgaptmp_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4469498.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVKRPVAITIVVVVMLITLTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFL"
     gene            828..2045
                     /locus_tag="ACHOQQ_RS06115"
                     /old_locus_tag="pgaptmp_001223"
     CDS             828..2045
                     /locus_tag="ACHOQQ_RS06115"
                     /old_locus_tag="pgaptmp_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4469499.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMAVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(2049..2606)
                     /gene="dcrB"
                     /locus_tag="ACHOQQ_RS06120"
                     /old_locus_tag="pgaptmp_001224"
     CDS             complement(2049..2606)
                     /gene="dcrB"
                     /locus_tag="ACHOQQ_RS06120"
                     /old_locus_tag="pgaptmp_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGU4469500.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(2679..3344)
                     /locus_tag="ACHOQQ_RS06125"
                     /old_locus_tag="pgaptmp_001225"
     CDS             complement(2679..3344)
                     /locus_tag="ACHOQQ_RS06125"
                     /old_locus_tag="pgaptmp_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGU4469501.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLTDKSEINAL
                     QAS"
     gene            3565..3810
                     /gene="tusA"
                     /locus_tag="ACHOQQ_RS06130"
                     /old_locus_tag="pgaptmp_001226"
     CDS             3565..3810
                     /gene="tusA"
                     /locus_tag="ACHOQQ_RS06130"
                     /old_locus_tag="pgaptmp_001226"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGU4469502.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(3912..6110)
                     /gene="zntA"
                     /locus_tag="ACHOQQ_RS06135"
                     /old_locus_tag="pgaptmp_001227"
     CDS             complement(3912..6110)
                     /gene="zntA"
                     /locus_tag="ACHOQQ_RS06135"
                     /old_locus_tag="pgaptmp_001227"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGU4469503.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(6184..6810)
                     /locus_tag="ACHOQQ_RS06140"
                     /old_locus_tag="pgaptmp_001228"
     CDS             complement(6184..6810)
                     /locus_tag="ACHOQQ_RS06140"
                     /old_locus_tag="pgaptmp_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGU4469504.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISSFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            6951..7310
                     /locus_tag="ACHOQQ_RS06145"
                     /old_locus_tag="pgaptmp_001229"
     CDS             6951..7310
                     /locus_tag="ACHOQQ_RS06145"
                     /old_locus_tag="pgaptmp_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGU4469505.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(7313..7582)
                     /locus_tag="ACHOQQ_RS06150"
                     /old_locus_tag="pgaptmp_001230"
     CDS             complement(7313..7582)
                     /locus_tag="ACHOQQ_RS06150"
                     /old_locus_tag="pgaptmp_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGU4469506.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(7572..8168)
                     /gene="rsmD"
                     /locus_tag="ACHOQQ_RS06155"
                     /old_locus_tag="pgaptmp_001231"
     CDS             complement(7572..8168)
                     /gene="rsmD"
                     /locus_tag="ACHOQQ_RS06155"
                     /old_locus_tag="pgaptmp_001231"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGU4469507.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAKCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLYREKVAGQVAYRLYQREAQGESDAD"
     gene            8318..9814
                     /gene="ftsY"
                     /locus_tag="ACHOQQ_RS06160"
                     /old_locus_tag="pgaptmp_001232"
     CDS             8318..9814
                     /gene="ftsY"
                     /locus_tag="ACHOQQ_RS06160"
                     /old_locus_tag="pgaptmp_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGU4469508.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKDEM
                     GEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            9817..10485
                     /gene="ftsE"
                     /locus_tag="ACHOQQ_RS06165"
                     /old_locus_tag="pgaptmp_001233"
     CDS             9817..10485
                     /gene="ftsE"
                     /locus_tag="ACHOQQ_RS06165"
                     /old_locus_tag="pgaptmp_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGU4469509.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            10478..11536
                     /gene="ftsX"
                     /locus_tag="ACHOQQ_RS06170"
                     /old_locus_tag="pgaptmp_001234"
     CDS             10478..11536
                     /gene="ftsX"
                     /locus_tag="ACHOQQ_RS06170"
                     /old_locus_tag="pgaptmp_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGU4469510.1"
                     /translation="MNKRDAINHIRRFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            11781..12635
                     /gene="rpoH"
                     /locus_tag="ACHOQQ_RS06175"
                     /old_locus_tag="pgaptmp_001235"
     CDS             11781..12635
                     /gene="rpoH"
                     /locus_tag="ACHOQQ_RS06175"
                     /old_locus_tag="pgaptmp_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGU4469511.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            12906..14009
                     /gene="livJ"
                     /locus_tag="ACHOQQ_RS06180"
                     /old_locus_tag="pgaptmp_001236"
     CDS             12906..14009
                     /gene="livJ"
                     /locus_tag="ACHOQQ_RS06180"
                     /old_locus_tag="pgaptmp_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGU4469512.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(14209..14592)
                     /gene="panM"
                     /locus_tag="ACHOQQ_RS06185"
                     /old_locus_tag="pgaptmp_001237"
     CDS             complement(14209..14592)
                     /gene="panM"
                     /locus_tag="ACHOQQ_RS06185"
                     /old_locus_tag="pgaptmp_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGU4469513.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            15016..16125
                     /gene="livK"
                     /locus_tag="ACHOQQ_RS06190"
                     /old_locus_tag="pgaptmp_001238"
     CDS             15016..16125
                     /gene="livK"
                     /locus_tag="ACHOQQ_RS06190"
                     /old_locus_tag="pgaptmp_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGU4469514.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            16173..17099
                     /gene="livH"
                     /locus_tag="ACHOQQ_RS06195"
                     /old_locus_tag="pgaptmp_001239"
     CDS             16173..17099
                     /gene="livH"
                     /locus_tag="ACHOQQ_RS06195"
                     /old_locus_tag="pgaptmp_001239"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGU4469515.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            17096..18373
                     /gene="livM"
                     /locus_tag="ACHOQQ_RS06200"
                     /old_locus_tag="pgaptmp_001240"
     CDS             17096..18373
                     /gene="livM"
                     /locus_tag="ACHOQQ_RS06200"
                     /old_locus_tag="pgaptmp_001240"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGU4469516.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            18370..19137
                     /gene="livG"
                     /locus_tag="ACHOQQ_RS06205"
                     /old_locus_tag="pgaptmp_001241"
     CDS             18370..19137
                     /gene="livG"
                     /locus_tag="ACHOQQ_RS06205"
                     /old_locus_tag="pgaptmp_001241"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGU4469517.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            19139..19852
                     /gene="livF"
                     /locus_tag="ACHOQQ_RS06210"
                     /old_locus_tag="pgaptmp_001242"
     CDS             19139..19852
                     /gene="livF"
                     /locus_tag="ACHOQQ_RS06210"
                     /old_locus_tag="pgaptmp_001242"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGU4469518.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            19971..20717
                     /locus_tag="ACHOQQ_RS06215"
                     /old_locus_tag="pgaptmp_001243"
     CDS             19971..20717
                     /locus_tag="ACHOQQ_RS06215"
                     /old_locus_tag="pgaptmp_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4469519.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQNKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            21077..22393
                     /gene="ugpB"
                     /locus_tag="ACHOQQ_RS06220"
                     /old_locus_tag="pgaptmp_001244"
     CDS             21077..22393
                     /gene="ugpB"
                     /locus_tag="ACHOQQ_RS06220"
                     /old_locus_tag="pgaptmp_001244"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGU4469520.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            22491..23378
                     /gene="ugpA"
                     /locus_tag="ACHOQQ_RS06225"
                     /old_locus_tag="pgaptmp_001245"
     CDS             22491..23378
                     /gene="ugpA"
                     /locus_tag="ACHOQQ_RS06225"
                     /old_locus_tag="pgaptmp_001245"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGU4469521.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            23375..24220
                     /gene="ugpE"
                     /locus_tag="ACHOQQ_RS06230"
                     /old_locus_tag="pgaptmp_001246"
     CDS             23375..24220
                     /gene="ugpE"
                     /locus_tag="ACHOQQ_RS06230"
                     /old_locus_tag="pgaptmp_001246"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGU4469522.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            24222..25292
                     /gene="ugpC"
                     /locus_tag="ACHOQQ_RS06235"
                     /old_locus_tag="pgaptmp_001247"
     CDS             24222..25292
                     /gene="ugpC"
                     /locus_tag="ACHOQQ_RS06235"
                     /old_locus_tag="pgaptmp_001247"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGU4469523.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            25289..26032
                     /gene="ugpQ"
                     /locus_tag="ACHOQQ_RS06240"
                     /old_locus_tag="pgaptmp_001248"
     CDS             25289..26032
                     /gene="ugpQ"
                     /locus_tag="ACHOQQ_RS06240"
                     /old_locus_tag="pgaptmp_001248"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4469524.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNYKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(26019..26459)
                     /locus_tag="ACHOQQ_RS06245"
                     /old_locus_tag="pgaptmp_001249"
     CDS             complement(26019..26459)
                     /locus_tag="ACHOQQ_RS06245"
                     /old_locus_tag="pgaptmp_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGU4469525.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            26579..28324
                     /gene="ggt"
                     /locus_tag="ACHOQQ_RS06250"
                     /old_locus_tag="pgaptmp_001250"
     CDS             26579..28324
                     /gene="ggt"
                     /locus_tag="ACHOQQ_RS06250"
                     /old_locus_tag="pgaptmp_001250"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGU4469526.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT         6586 a         7257 c         7943 g         6614 t
ORIGIN      
        1 caaaaacgcc agtactgccc acatcagcgc aaactgcacc cccatcagct ccagccccag
       61 ccagacgatg acacctgtcc ataaactgag tagcgtcttt aacgccagat aatgcgaaac
      121 gcctttaagt gcgcggtgta atcctgcgat gtgaatctgt gggttattca atgcaaaacg
      181 cattttgtaa gggacgtggc gcacttcaaa cagcataaaa actacggtca tcaccagcaa
      241 aagcacgctc gccattgccc cggaaagccc ggtcattagc gctgtggtga aggtaaccac
      301 tttttccgag tccatccgct gcagcattcg ctccggcgac atatgcaaat taagaaaagg
      361 caacatctcc tgcaatttaa aaagtttgcg cgtcagctcc ttattaaact tcggcagcat
      421 agagataaat tcgttaaacg atgccgccag tacgccgacc agcgcggtta gtgtgatcag
      481 catcaccacc accacaatcg taatggcaac ggggcgtttt actccccgtc gaataaacca
      541 ggtaacgagc gggttgagga cgatggcaaa aaacagcgcc agtaacagct gcacaatgat
      601 atctgccgct gcgtgaatgc ccgcgaggat cactaccagc gaggccagct tgagcagaat
      661 gtgcatgccc gttttatcgg gttgaggggt ttccattggg gcttccttgt gactttttgt
      721 attaagtgta gcgggagtca cgccagcaat attctgattc ttactgctga ttttcacgtc
      781 agcacgtggt aaaaatgaaa cactgttgta aaaatgtggt gatcctcatg cccgaacccg
      841 tagccgaacc cgcgctaaac ggattgcgcc tgaatttgcg cattgtctcc attgtcatgt
      901 ttaacttcgc cagctacctc accatcgggt tgccgctcgc tgtattaccg ggctatgtcc
      961 atgatgtgat gggctttagc gctttctggg caggattggt tatcagcctg caatattttg
     1021 ccaccttgct gagccgtcct catgccggac gttacgccga tttgctgggc cccaaaaaga
     1081 ttgtcgtctt cggtttatgc ggctgctttt tgagcggtct gggatatctg acggcaggat
     1141 taaccgccag tctgcccgtc atcagcctgt tattactttg cctgggacgc gtcatccttg
     1201 ggattgggca aagttttgcc ggaacgggat cgaccctgtg gggcgttggc gtggttggct
     1261 cgctgcatat cggacgggtg atttcgtgga acggtattgt cacttatggg gcgatggcga
     1321 tgggtgcgcc gttaggtgtc gtgttttatc actggggcgg cttgcaggcg ttagcgttaa
     1381 tcattatggc cgtggcgctg gtggccattt tgttggcgat cccgcgtccg acggtaaaag
     1441 ccagtaaagg caaaccgctg ccgtttcgcg cggtgcttgg gcgcgtctgg ctgtacggta
     1501 tggcgctggc gttggcttcc gccggatttg gcgtcatcgc cacctttatc acgctgtttt
     1561 atgacgctaa aggttgggac ggtgcggctt tcgcgctgac gctgtttagc tgtgcgtttg
     1621 tcggtacgcg tttgttattc cctaacggca ttaaccgtat cggcggctta aacgtagcga
     1681 tgatttgctt tagcgttgag ataatcggcc tgctactggt tggcgtggcg actatgccgt
     1741 ggatggcgaa aatcggcgtc ttactggcgg gggccgggtt ttcgctggtg ttcccggcat
     1801 tgggtgtagt ggcggtaaaa gcggttccgc agcaaaatca gggggcggcg ctggcaactt
     1861 acaccgtatt tatggattta tcgcttggcg tgactggacc actggctggg ctggtgatga
     1921 gctgggcggg cgtaccggtg atttatctgg cggcggcggg actggtcgca atcgcgttat
     1981 tactgacgtg gcgattaaaa aaacggcctc cggaacacgt ccctgaggcc gcctcatcat
     2041 cttaaaactt actgaataac cagcgtatta atgatgtttt ctgcggtggt ctgcgctttt
     2101 tgctgatcgt cagctggcag agtaatttgc atggtcagca gttgattacc cacgttaccc
     2161 agaataacgg aagagtacgc cgtctggcct ttcgcggaga taatgctgtc taactgctgc
     2221 attttgtgac ctttcagctc aatggctttg ttggtcacta cttgcagctg cggatcgcgg
     2281 ctacgttgct gatcttccag acgcttcgcc agcaccgcca gatcttcttt cggatcatcg
     2341 cccatgatga cgatgactgc tttctgcccg gtggcgtcgg accagacatg catgttattg
     2401 gcctgcgttc ccagcttacc gctctggtcg gtcatatccg ctggcagcga gaaacttaac
     2461 ttgccatcaa gcaggttgac gggattcccg gtagcgttac tttccgcgac cgaaccctgc
     2521 gccgtagcgt tagtgtcttt atcatcacag gccgcaagcc ccataaccag caggccaatt
     2581 ccgacatatt taaccagatt gcgcattgac ttcttccttt cgataaacgg ccataacggc
     2641 tcattcatcc atcttatcac aactctgata acgaaccttt aactcgcctg caaagcgttg
     2701 atttcggatt tatctgtcag tcttttcaac agcatattga gtaatacgcc atacattggc
     2761 aggaagaaaa cgatactgat taacactttg aaacagtaat cgaccagcgc gatttccatc
     2821 cagtgttcag ccataaaggc atccgggcta cgccagaagg caatgaagaa aaaggccagc
     2881 gtgtcgctga cgttaccgaa cagtgtggac gctgtcggtg ccagccacca gcggcgactc
     2941 tgacgcaggc ggttaaaaac gtgcacgtcg aggatctgtc ccagcgcgta ggccatgaaa
     3001 ctggcggtgg cgatacgggc gacaaacagg ttgaagtggg cgagtgcgcc gaatccctgc
     3061 caggaaccca tatagaatag cgacgagatg acgtaggaga tcagcaatgc cgggatcatc
     3121 accgcaaaga tgatacgacg ggccagcggt gcgccaaaaa tacgtacggt caggtcggta
     3181 gcaaggaaaa taaacgggaa gctaaacgcg ccccaggtgg tatggaaacc aaaaatggag
     3241 acgggaagct gaaccagata gttactggag gtgatcacca gcagatgaaa taacgataac
     3301 cagaacaacg ccttatagcg ttgagtttgc gagaaaacgt tcatattgta cctttttgat
     3361 taaccattgg ggtgagggaa cccaatacgt acgacacgtc tgttacctta acataataac
     3421 gtgcttaccc ttttttcgag ccgccgcatg atactgcttt acgttgacaa tgcaatggtt
     3481 aattttcacg caatcgttaa cctggtttgc ttacggactc acagggcgta aactagcgcc
     3541 gtttttttat gtgatgagaa gaaaatgacc gatctctttt ccagccctga ccacacactc
     3601 gacgcacttg gcctgcgctg cccggaaccc gtaatgatgg tgcgcaaaac agtgcgcaat
     3661 atgcagcctg gtgaaacgtt gctgattatt gctgacgatc cggcgacgac ccgcgatatt
     3721 cctgggtttt gtacctttat ggaacacgaa ctggttgcta aagagacgga tggactgcct
     3781 tatcgttatt tgattcgtaa aggcggttga tgggggcaga ttggcttcga tgccgccttt
     3841 tcccctcacc ctaaccctct ccccagagga gcgaggggac cgatcgcgct caatgttgcg
     3901 atcggtttgc cttatctcct gcgcaacaat cttaacgcat tcgctgtcac cagcaccgtc
     3961 gcacccgtat ctgccagcac cgccagccat agtccggtca ttcctaacag cgtggtgacg
     4021 aggaagatcc ctttcagccc cagcgcaata gtgatgttct ggcggatatt ggcgtgagtg
     4081 gcgcgtgcca gttcaatcat ttgcaccaga ccgcgcaggt ggttatgggt taatgctgcg
     4141 tcggcggttt ccagcgccac gtctgtgccg ctgcccattg caatcccgat ggcggcagct
     4201 ttcatcgctg gcgcgtcgtt aataccgtca ccgaccatcg ccagcggcgc atgttgattc
     4261 agctcggtta ccgctttgac tttatcttcc ggcaacaggc ccgctttaaa ctccagcccc
     4321 agctccccgg caattgccgc cgctgcgcgt ggattatcgc cggtgaggat cacccctttg
     4381 acgcccagcg cgttcagttc actgatggca gttgcagcat cggcgcgcag ggtatcctgt
     4441 aacgcaatga cacccagcac gtcatcgtta cgtactacca gcactaccgt ttgcccggcg
     4501 ctttccagtt cgttaattaa accagcaaat gcatcagcgg gatgtttccc ggcagcgcaa
     4561 atcaatacgc gctcaccgtt aacctgcgct tcaatgccag acccgaccag cgcccgctgt
     4621 gattcggcgg cgggaatggc gagtgcagca acctgtgctt cgcgcacgat ggcctgcgcc
     4681 agtggatgcg tcgcgccttg ctcgaccgcc gccgccagtg tcagcagttc agattcacta
     4741 atacccgttg ccggatgaat cgcggtaacg cgcggtttac cgacggtcag cgtaccggtt
     4801 ttatcaaacg ccacctgggt aacacgacct agctgctcca gcgccgcacc gcctttaatt
     4861 aacgccccac gacgtgccgc tgctgccagc ccggaggtaa tcgccgcggg cgtggagata
     4921 actaacgcac acgggcagcc aatcagcagc agcgtcaggc ctttataaat ccactcttgc
     4981 cagctggcgg caaacagcag cggcggcacc agcgttacca gcagggcgac ggccataatc
     5041 gctggcgtat aaatacggct gaaacggtcg ataaaccgct caatgggagc gcgacgctct
     5101 tcggcttctt cgatcagttt cagaatccgg tcaatggcgc tggctcccgg ttcggacaat
     5161 acttccagcg ttaccagacg gtctacgctg gtggctcctg caggaacttt atcgcccgtt
     5221 gcgcgctcca ccggaatgga ttcgccggtc agggcgcttt catcaaaact ggcaaagggc
     5281 gagagcagtt taccgtcggc aggcaaacgc ccgcccgccg caacttcaat cacatcgcca
     5341 gggcgcaggc tgttaatcgc cacctcttcc cgctcaccgt tacgcaggcg cgtggcggtt
     5401 tctggtttca gcgccatcaa cgcgctaacg ccctgacgcg cgcggctggc ggcccagcct
     5461 tccagtcgtt caccaatcaa aaacagcagc aacaccatcg cagcttcagc cgttgcgcca
     5521 ataaacagcg caccaatagc agctacgctc attaacgttt caatggcgaa gtaactgcct
     5581 gatttgatca gccgtaatgc ctgacgagca atcgggtaca gcccaaccag cgtggtcgcg
     5641 ataaacgcca gttgaccgaa cggatgatta aattgctcca gtccccagct gattgccatc
     5701 atcacgataa gcgtaatcag cggcagattc tctttcaggc gcgattcttg tggctcgtcg
     5761 gcggcctgct catcgcgcag ggaatagccc gctttttgca ccgcagattc aacctgggca
     5821 cggatgtcat tgtcggcatc aaccaccagt ttttcggtgg cgaacagtac ctgcacctga
     5881 ttcacgcctg caagctggcg cacggcattt tctaccttgc gcgcacaggc ggcgcagtcc
     5941 atgccgctga ctttccagct atagcgggtg ccggagacgt tttcagagag cgttggcgtg
     6001 ctggaacatg cgccgtcgca gcaacagtcg ttggcgttct gtaccgtggt tagcggtttg
     6061 aacgcagcaa attgaggggc tttcttgccg tgattgtcag gagtcgacat ggcatcctcc
     6121 ggttaagttt tttctcatta accgaaggat acactctgga gtcgactcca gagtcaagtt
     6181 ttatcagaga tacagcgagc ggacgatgag gaagtgaccg gcaaagtagc aggccgcagc
     6241 aatcgcgtta tccgcacgga agcgtcggcg atagtggcta cccagccaga caaagctact
     6301 gataaacagc aacgaagcgc cgacaaacgc agagagtgcc ggagcggtcg gacggaagaa
     6361 ccacagttca cctgccagcc acaccatcac cagcgtcatg ccgataaacg tgcagatagg
     6421 ccaacgatac tcttccagcc gcgtccagat aatcgccagt aacagcgcgc ccagaaccag
     6481 cagtaccaac ggcagaggcc agaagaaaga gagcgtcatc tgactggcga aatagatggt
     6541 gtacagcagg tgcgagagga aaaacgcgcc gatggcgtac atcagacgtt gacgtggcaa
     6601 cagggttagc gcatcgccca gcagtgaggc gcacagccct gccagcacca gatagctaat
     6661 ggcgtcgaac atcggcgctt gccaggccag taacagcagg agaagaaggg ttaacggttt
     6721 aaacacccag cgttgccagg ttggcccacg atacgatgca tccacagata gccatgcgga
     6781 aagacagaca gcgataaacg accaaagcat cttagttcct tgaattcgtt attttttcgt
     6841 aggcggaatg cctacggtct tgcttccagt ttagtggcga gggtggacgg atgacaacac
     6901 cataactggc agggtatgct taattccgca ttttccgatg agggataaag atgagcaaac
     6961 cacctctttt ctttattgtt atcattggct taatcatcgt cgccgcatcg tttcgtttta
     7021 tgcaacagcg acgggaaaaa gctgataatg atatggcacc gctccagcaa aagctggtgg
     7081 tggtgagcaa caagcgggaa aaaccgatta acgatcgccg ttcgcgtcag caggaagtga
     7141 ctccggcagg caccagtatg cgctatgagg caagcttcaa accgcaaagc ggaggaatgg
     7201 agcagacgtt tcgcctcgac gcccagcagt accacgccct gacagtgggc gataaaggta
     7261 cgctgagcta taaaggaacg cgctttgtta gctttgttgg cgaacaataa ggttattttt
     7321 taactttaaa tttcttctgc cagaccagca gttcaaacac gccgaaaagg aaaattcgca
     7381 ccttttcggc ggtggtcatc tgcgggccat ctttcggtaa agtggatttc aatagcgcca
     7441 gctgcatacc atgcatcagc acggtaaaaa tcagcgccac gttaacgaaa atattcagcg
     7501 ggcgcgggaa gggatgcacc aggttgagga ttaaaaatcc ccaaacgcag agcattaaca
     7561 aacgtccaat attaatcagc atcactttct ccttgtgctt cgcgttgata cagccgataa
     7621 gccacctgac ccgccacttt ttcccgatac aatgaccagt ttgccggaac agtgggcaga
     7681 ccgttttcga cttcgctttc gacataaatc agggcttcgt cagccagcca gccgttatct
     7741 tccagtaaat ttatcgtctc ttctaacaag ccacggcgga acggtggatc gacaaacacg
     7801 atattatgcg gtgtgccttt ttgcgccagg aatgacatcg cgttgctgtt cactacgcgt
     7861 gcattgcctg cttttagtgt cgccagattc ttaattaact gctgagaaac tgcacgatcc
     7921 atctcaatca acgtggcccc cgcagcgtag cgtgataacg cttccagccc cagcgcgccg
     7981 cttccggcga agcaatccag acatttggcg tcaacaatga ccggagccag ccagttaaat
     8041 aacgtttcgc gtacgcggtc ggtggtgggg cgcagacctg ggctatcagg aaccgggagt
     8101 ttacggcctc gccactgccc gccaataatg cggatttggc cgctgccgga atgattcggt
     8161 tttttcatga taaattgctc aatccgccag ataacacata cttgcaggcg gtgatgtgaa
     8221 ttaagttaag tgatagacta tttcatcatt tttttagctg ctatgtacat agcgttaacg
     8281 ctgtgccatg aagcaacagc gaggagtgta gtcgcaaatg gcgaaagaaa aaaaacgtgg
     8341 ctttttttcc tggctgggct ttggtcaaaa agagcagacc ccggaaaaag agacagaagt
     8401 tcagaatgaa caaccggttg tagaagaaat cgttcaggcg caagagcctg tgaaggcttc
     8461 ggaacaagcc gttgaagagc agccgcaggc gcatactgaa gccgaggcgg aaacttttgc
     8521 tgccgacgtt gtggaagtca ctgaacaggt agctgaaagt gaaaaagcgc agcctgaagc
     8581 ggaagtcgtt gcacagccgg aaccggtcgt agaagaaacg ccggagccag tggctatcga
     8641 acgtgaagaa ctgccgttgc cggaagacgt caacgccgaa gaggtttcgc cagaagagtg
     8701 gcaggctgaa gcggaaaccg tagagattgt cgaagcggcg gaagaagaag cggctaaaga
     8761 agaaattacc gacgaagagc tggaagcaca ggcgctggct gccgaagcgg cagaagaggc
     8821 ggtgatggtg gttcctccgg cagaagaaga gcagccggtg gaagaaatcg ctcaggagca
     8881 ggaaaaaccg accaaagaag gttttttcgc gcgcctgaaa cgcagcctgt taaaaaccaa
     8941 agaaaatctc ggttccggat ttatcagcct gttccgcggt aaaaaaatcg acgatgatct
     9001 gtttgaggag ctggaagagc agcttttgat cgccgatgtg ggtgtggaaa ccacacgtaa
     9061 aattatcacc aatctgacgg aaggcgcatc ccgcaagcag cttcgtgatg ccgaggcgct
     9121 ctatggcctg ctgaaagacg agatgggcga gattctggcg aaagtcgatg agccgctgaa
     9181 tgttgaaggc aaagcgccgt ttgtgatcct gatggtgggc gtcaacggtg tgggtaaaac
     9241 cacgacgatt ggtaagctgg cacgtcagtt tgagcagcag ggtaaatcgg tgatgctggc
     9301 ggcgggcgat actttccgtg cagcagcggt tgaacagctt caggtctggg gtcagcgcaa
     9361 caatattccg gtgattgccc agcataccgg tgcggattcc gcctctgtta tcttcgacgc
     9421 cattcaggca gctaaagcgc gtaatatcga cgtcctgatt gccgataccg ccggacgtct
     9481 gcagaacaaa tcgcatctga tggaagagtt gaagaaaatc gtccgcgtga tgaagaaact
     9541 cgacgttgaa gcgccgcatg aagttatgct gactattgat gccagcaccg ggcagaacgc
     9601 ggtaagccag gccaaactgt tccacgaagc tgttggctta accggcatca cgctaacgaa
     9661 actggacggc acggcgaaag gcggggtaat tttctcggtg gctgaccagt ttggtatccc
     9721 tatccgctac attggtgtcg gcgaacgtat tgaggatttg cgtccgttta aggcggacga
     9781 ctttatagag gcactttttg cccgagagga ttaacaatga ttcgctttga acatgtcagc
     9841 aaggcttatc tcggtgggag acaggcgctg cagggcgtta cgttccatat gcagccgggt
     9901 gagatggcgt ttctgaccgg tcattccggc gcagggaaaa gtaccctcct gaagctgatc
     9961 tgtgggattg agcggcccag cgccgggaaa atctggttta gcggccatga catcacgcgt
    10021 ctgaaaaacc gtgaagttcc gtttctgcgc cgccagattg gcatgatttt ccaggatcac
    10081 catctactga tggaccgtac tgtctacgat aacgtggcga tcccgctgat tatcgccggt
    10141 gccagcggtg acgatattcg tcgccgggtg tcggcggcac tggataaagt cgggctactg
    10201 gacaaagcga agaacttccc tattcagctt tctggcggtg aacaacagcg tgttggcatt
    10261 gcccgcgcgg tggtgaataa gcccgcggta ctgctggcgg acgaaccgac tggtaacctg
    10321 gacgacgcgt tgtcggaagg gattctacgt ctgtttgaag agtttaaccg cgttggggta
    10381 accgtattga tggcaacgca cgacatcaac ctgatttcgc ggcgttccta tcgcatgctc
    10441 accctgagcg atggtcactt gcatggaggc gtgggccatg aataagcgcg atgcaatcaa
    10501 tcatattcgg cggtttggcg ggcgtctgga tcgcttccgt aaatcggtcg gcggctcagg
    10561 cgacggcggt cgtaacgcgc cgaaacgcgc gaaatcctcg ccaaaaccgg taaatcgcaa
    10621 aaccaacgtt ttcaacgaac aggtgcgcta tgccttccac ggcgcattgc aggatctgaa
    10681 aagcaaaccg ttcgccacgt ttttaacggt gatggttatc gccatctctc tgacgctgcc
    10741 cagcgtctgt tatatggtgt acaaaaacgt taaccaggcg gcgacgcaat attatccgtc
    10801 accgcaaatc actgtttatc tgcaaaaaac gctggacgat gacgctgctg cgggcgtggt
    10861 ggcacagttg caggccgagc agggcgtgga gaaagtgaat tatctttctc gtgaagacgc
    10921 actgggcgag ttccgtaact ggtctggttt tggtggtgca ctggatatgc tggaagaaaa
    10981 cccgcttccg gcagtggcgg tggtgatccc gaaactcgat tttcagggga cggaatcact
    11041 caatacgctg cgtgatcgta tcacgcagat taacggcatt gacgaagtgc ggatggatga
    11101 cagctggttt gcccgtctgg cggcgttgac cgggctggtc gggcgcgttt cggcgatgat
    11161 cggcgtgttg atggtggcgg cagtgttcct cgtcatcggt aacagtgtac gcctgagtat
    11221 ctttgctcgc cgtgactcca tcaacgtgca gaaactgatt ggtgcgacgg atggtttcat
    11281 cctgcgtccg ttcctgtacg gtggcgcact gctgggattt tctggcgcat tgttgtcatt
    11341 aattttgtca gaaattctgg tgctgcgatt gtcatcggcg gttgcggaag tggcacaggt
    11401 tttcggaacg aagtttgata tcaatggttt atcgttcgat gaatgcctgc tgttgctgct
    11461 ggtatgctcg atgattggct gggtggcagc gtggcttgcc acggtacaac atttacgcca
    11521 ctttacgcct gaataataaa agcgtgttat actctttccc tgcaatgggt tccgtagcag
    11581 ggaaagagac cccgttgtct cttcccggta tttcatctct atgtcacatt ttgtgcgtaa
    11641 tttattcaca agcttgcatt gaacttgtgg ataaaatcac ggtctgataa aacagtgaat
    11701 gataacctcg ttgctcttaa gctctggcac agttgttgct accactgaag cgccagaaga
    11761 tatcgattga gaggatttga atgactgaca aaatgcaaag tttagcttta gccccagttg
    11821 gcaacctgga ttcctacatc cgggcagcta acgcgtggcc gatgttgtcg gctgacgagg
    11881 agcgggcgct ggctgaaaag ctgcattacc atggcgatct ggaagcagct aaaacgctga
    11941 tcctgtctca cctgcggttt gttgttcata ttgctcgtaa ttatgcgggc tatggcctgc
    12001 cacaggcgga tttgattcag gaaggtaaca tcggcctgat gaaagcagtg cgccgtttta
    12061 acccggaagt gggtgtgcgc ctggtctcct tcgccgttca ctggatcaaa gcagagatcc
    12121 acgaatacgt tctgcgtaac tggcgtatcg tcaaagttgc gaccaccaaa gcgcagcgca
    12181 aactgttctt caacctgcgt aaaaccaagc agcgtttggg ctggtttaac caggatgaag
    12241 tcgaaatggt ggcccgtgaa ctgggcgtaa ccagcaaaga cgtacgtgag atggaatcac
    12301 gtatggcggc acaggacatg acctttgacc tgtcttccga cgacgattcc gacagccaac
    12361 cgatggcacc ggtgctctat ctgcaggata aatcatctaa ctttgccgac ggcatcgaag
    12421 atgataactg ggaagagcag gcggcaaacc gtctgaccga cgcgatgcag ggtctggacg
    12481 aacgcagcca ggacatcatc cgtgcgcgct ggctggacga agacaacaag tccacgttgc
    12541 aggaactggc tgaccgttac ggtgtttccg ctgaacgtgt acgccagctg gaaaagaacg
    12601 cgatgaaaaa actgcgcgct gctatagaag cgtaatttcc gctattaagc agagaaccct
    12661 ggatgcgagt ccggggtttt tgttttttgg gcctcaacaa taatcaattt cccctccgac
    12721 aaaacgccaa tccccacgca gattgttaat aaactgtcaa aatagctatt ccaatatcat
    12781 aaaaatcggg tatgttttag cagagtatgc tgctaaagca cgggtagtca tgcataaaac
    12841 gaaataaagt gctgaacaac aacatcacaa cacacgtaat aaccagaaga atggggattc
    12901 tcaggatgaa cataaagggt aaagcgttac tggcaggatg tatcgcgctg gcattcagca
    12961 atatggctct ggcagaagat attaaagtcg cggtcgtggg cgcaatgtcc ggtccggttg
    13021 cgcagtacgg tgaccaggag tttaccggcg cagagcaggc ggttgcggat atcaacgcta
    13081 aaggcggcat taaaggcaac aaactgcaaa tcgtaaaata tgacgatgcc tgtgacccga
    13141 aacaggcggt tgcggtggcg aacaaagtcg ttaacgacgg cattaaatat gtgattggtc
    13201 acctctgttc ttcatcaacg cagcctgcgt ctgacatcta tgaagacgaa ggcattttaa
    13261 tgatcacccc agcggcaacc gcgccggagc tgaccgcccg tggctatcag ctgatcctgc
    13321 gcaccaccgg cctggactcc gaccaggggc cgacggcggc gaaatatatt cttgagaaag
    13381 tgaaaccgca gcgtattgct atcgttcacg acaaacagca atacggcgaa ggtctggcgc
    13441 gtgcggtgca ggacggcctg aagaaaggca atgctaacgt ggtgttcttt gatggtatca
    13501 ccgccgggga aaaagatttc tcaacgctgg tagcgcgtct gaagaaagag aatatcgact
    13561 tcgtttacta cggcggttat cacccggaaa tggggcaaat cctgcgtcag gcacgcgcgg
    13621 cagggttgaa aactcagttt atggggccgg aaggcgtggc taacgtctcg ctgtctaaca
    13681 ttgcgggcga atcagcggaa gggctgctgg tgaccaagcc gaagaactac gatcaggttc
    13741 cggcgaacaa acccattgtt gatgcgatca aagcgaaaaa acaggaccca agcggcgcat
    13801 tcgtttggac cacctacgcc gcgctgcaat ctttgcaggc gggcctgaat cagtctgacg
    13861 atccggctga gatcgccaaa tacctgaaag cgaactcggt tgataccgtg atgggtccgc
    13921 tgacgtggga tgagaaaggt gatctgaaag gctttgagtt cggtgtgttt gactggcacg
    13981 ccaacggcac agcgaccgat gcgaagtaat tgttaatcgg caactttggg ttgccgccaa
    14041 attgctaata tagagtacgt tgcttcatgc cggatgcggc gtaaacgcct tatccggcct
    14101 acaagatcca aagaaatcag taaattgcaa cacacattgt aggcctgata agcgtagcgc
    14161 atcaggcaat ttctcgttgg tcatcacttt tgaaatcaaa cttgacgatt aacgcttctc
    14221 ccagccgccc tgctgtgccg taaatcccag tgcctgcata aacgccgtca tcacaccgcg
    14281 atcttccacg cctgcatccg ccatccacca gcatgaaacg ccaggattgt tacgcaaaac
    14341 ctcttccagc agatattgcc ccacaccgcg acgtcgagtg acttcccgca cgcgcaggga
    14401 atccagtgcc ccctcggtgc cgcttaaggt tacccgcacg gccgcaagca ggcgctcgtt
    14461 aaaacgcgcg gcgtagatgc ggtggttatc gtcaacctgt aaagaggaag gggaatactc
    14521 cggccagatc ttttgcaggt caatccggtc ctggtcgcta aatttttcta atcgaatgat
    14581 ggtcagcttc atgggtaacc cgtgtaaatc acaaaagtgg aaccagtgta gcgaaataat
    14641 ttaatcggag gctttctctt ttttatttct tttggcaggt gattaatttt ttaacagcaa
    14701 taattacaaa gttaaaacat tagagaatga aaaatgtcca gcataatccc ctgaatgata
    14761 gtgaattatt ccgccctttg tgccgttatt ttatgctgac aaaggcactt ttttctgttt
    14821 atctatcaat aaattcagaa tattatctgt tcttaatcga ctgaaaaata gggattttaa
    14881 tcgctatcat cacaaaatac tgcgctaacc ccttaatcag acaggcaaaa acagtgcagt
    14941 ataaaaaaag aacagtctga tttgttaaca cataaaaaca aagcaacaca acatcacgaa
    15001 tggggatttt tgactatgaa acggaatgcg aaaactatca tcgcagggat gattgcactg
    15061 gcaatttcac acaccgctat ggctgacgat attaaagtcg ccgttgtcgg cgcgatgtcc
    15121 ggcccgattg cccagtgggg cgatatggaa tttaacggcg cgcgtcaggc gattaaagac
    15181 attaatgcca aaggggggat taaaggcgat aagctggttg gcgtggaata tgacgacgcc
    15241 tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg ttaacgacgg cattaaatac
    15301 gttattggtc atctgtgttc ttcttctact caacctgcat cagatatcta tgaagacgaa
    15361 ggtattctga tgatctcgcc gggagcgacc aacccggagc tgacccaacg cggttatcaa
    15421 cacattatgc gtactgccgg gctggactct tcccaggggc caacggcggc aaaatacatt
    15481 cttgagacgg tgaagcccca gcgcatcgcc atcattcacg acaaacaaca gtatggcgaa
    15541 gggctggcgc gttcggtgca ggacgggctg aaagcggcta acgccaacgt cgtcttcttc
    15601 gacggcatta ccgccggcga gaaagatttc tccgcgctga tcgcacgcct gaaaaaagaa
    15661 aacatcgact tcgtttacta cggcggttac tacccggaaa tggggcagat gctgcgccag
    15721 gcccgttccg ttggcctgaa aactcagttt atggggccgg aaggtgtggg taacgcatca
    15781 ttgtcgaata ttgccggtga tgctgccgaa ggcatgttgg tcactatgcc aaaacgctat
    15841 gaccaggatc cggcaaacca gggcatcgtt gatgcgctga aagcagacaa gaaagatccg
    15901 tccgggcctt atgtctggat cacctacgcg gcggtgcaat ctctggcaac tgcccttgag
    15961 cgtaccggca gcgatgagcc gctggcgctg gtgaaagatt taaaagctaa cggtgcaaac
    16021 accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc ttaagggatt tgattttggt
    16081 gtcttccagt ggcacgccga tggttcatcc acggcagcca agtgatcatc ccaccgcccg
    16141 tgaaatgcgg gcgggtttag aaaggttacc ttatgtctga gcagtttttg tatttcttgc
    16201 agcagatgtt taacggcgtc acgctgggca gtacctacgc gctgatagcc atcggctaca
    16261 ccatggttta cggcattatc ggcatgatca acttcgccca cggcgaggtt tacatgatcg
    16321 gcagctacgt ctcatttatg atcatcgccg cactgatgat gatgggcatt gataccggct
    16381 ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat tgccagcgcc tacggctgga
    16441 gtatcgaacg ggtggcctat cgcccggtgc gtaactccaa gcgcctgatt gcactcatct
    16501 cggcaatcgg tatgtccatc ttcctgcaaa actacgtcag cctgaccgaa ggttcgcgcg
    16561 acgtggcgct gccgagcctg tttaacggtc agtgggtggt gggccatagc gaaaacttct
    16621 ctgcctctat taccaccatg caggcggtga tctggattgt caccttcctc gccatgctgg
    16681 cgctgacgat tttcattcgt tattcccgta tggggcgcgc ctgccgtgcc tgcgccgaag
    16741 atctgaaaat ggcgagtctg cttggcatta acaccgaccg ggtgattgcg ctgacctttg
    16801 tgattggcgc ggcgatggcg gcggtggcgg gtgtgctgct cggtcagttc tacggcgtca
    16861 ttaaccccta tatcggcttt atggccggga tgaaagcctt taccgctgcg gtgctcggtg
    16921 ggattggcag cattccggga gcgatgattg gcgggctgat tctggggatt gcggaggcgc
    16981 tctcttctgc ctatctgagt acggaatata aagatgtggt ctcattcgcc ctgctgattc
    17041 tggtgctgct ggtgatgccg accggtattc tgggtcgccc ggaggtagag aaagtatgaa
    17101 accgatgcat attgcaatgg cgttgctctc tgccgcgatg ttttttgtcc tggcgggcgt
    17161 ctttatgggc gtacaactgg agctggatgg caccaaactg gtggtcgaca cggcttcaga
    17221 catccgttgg cagtgggtgt ttatcggcac ggcggtggtc tttttcttcc agcttttgcg
    17281 accggctttc cagaaagggt tgaaaagcgt ttccggaccg aagtttattc tgcccgccat
    17341 tgatggctcc acggtgaagc aaaagctgtt tctcgtggcg ctgctggtgc ttgcggtggc
    17401 gtggccgttt atggtctcac gcgggacggt ggatatcgcc accctgacca tgatctacat
    17461 tatcctcgga ctcgggctga acgtggttgt tggtctttct ggtctgctgg tgctggggta
    17521 cggcggcttt tacgccatcg gcgcttacac ttttgcgctg ctcaatcact attacggttt
    17581 gggcttctgg acctgtctgc cgattgccgg gttaatggcg gcggcggcgg gcttcctgct
    17641 cggcttcccg gtgcttcgct tgcgcggcga ctatctggcg atcgttaccc tcggtttcgg
    17701 cgaaattgtg cgcatattgc tgctcaataa caccgaaatt accggcggcc cgaacggaat
    17761 cagtcagatc ccgaaaccga cactcttcgg actcgagttc agccgtaccg ctcgtgaagg
    17821 cggctgggac acgttcagta atttctttgg cctgaaatac gatccctccg atcgtgtcat
    17881 cttcctctac ctggtggcgt tgctgctggt ggtgctaagc ctgtttgtca ttaaccgcct
    17941 gctgcggatg ccgctggggc gtgcgtggga agcgttgcgt gaagatgaaa tcgcctgccg
    18001 ttcgctgggc ttaagcccgc gtcgtatcaa gctgaccgcc tttaccatca gtgccgcgtt
    18061 tgccggtttt gccggaacgc tgtttgctgc gcgccagggc tttgtcagcc cggaatcctt
    18121 cacctttgcc gaatcagcct ttgtactggc gatagtggtg ctcggcggta tgggctcgca
    18181 atttgcagtg attctggcgg ctgttctgct ggtggtatcg cgcgagttga tgcgtgattt
    18241 caacgaatac agcatgttaa tgctcggtgg tttgatggtg ctgatgatga tctggcgtcc
    18301 gcagggcttg ctgcccatga cgcgcccgca actgaagctg aaaaacggcg cagcgaaagg
    18361 agagcaggca tgagtcagcc attattatct gttaacggcc tgatgatgcg cttcggcggc
    18421 ctgctggcgg tgaacaacgt caatcttgaa ctgtacccgc aggagatcgt ctcgttaatc
    18481 ggccctaacg gtgccggaaa aaccacggtc ttcaactgcc tgaccggttt ctacaaaccc
    18541 accggcggca ccattttact gcgcgatcag catctggaag gtttgccggg gcagcaaatc
    18601 gcccgcatgg gcgtggtgcg taccttccag catgtgcgtc tgttccgtga aatgacggta
    18661 attgaaaacc tgctggtggc gcagcatcag caactgaaaa ccgggctgtt ctcaggcctg
    18721 ttgaaaacgc cgtctttccg tcgcgcccaa agtgaagcgc tcgaccgcgc cgccacctgg
    18781 cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg cgagtaacct ggcctacggt
    18841 gaccagcgcc gtctggagat cgcccgctgc atggtgacgc agccggagat tttaatgctc
    18901 gacgaacctg cggcaggtct taacccgaaa gaaacgaaag agctggatga gctgattgcc
    18961 gaactgcgca atcatcacaa caccactatc ttgttgattg aacacgatat gaagctggtg
    19021 atgggaattt cggaccgaat ttacgtggtc aatcagggga cgccgctggc aaacggtacg
    19081 ccggagcaaa tccgtaataa cccggacgtg atccgtgcct atttaggtga ggcataagat
    19141 ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac tacggcaaaa tccaggcgct
    19201 gcatgaggtg agcctgcata tcaatcaggg cgagattgtc acgctgattg gcgcgaacgg
    19261 ggcggggaaa accaccttgc tcggcacgtt atgcggcgat ccgcgcgcca ccagcgggcg
    19321 aattgtgttt gatgataaag acattaccga ctggcagacg gcgaaaatca tgcgcgaagc
    19381 ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg atgacggtgg aagagaacct
    19441 ggcgatgggc ggcttttttg ccgaacgtga tcagtttcag gagcgcataa agtgggtgta
    19501 tgagctgttt ccacgtcttc atgagcgtcg tgttcagcgg gcaggcacca tgtccggcgg
    19561 tgaacagcag atgctggcga ttggtcgcgc gctgatgagc aatccgcgtt tgttgttgct
    19621 tgatgagcca tcgctcggtc ttgcgccgat tatcatccag caaattttcg acaccatcga
    19681 gcagctgcgc gagcagggga tgactatctt cctcgtcgag cagaacgcca accaggcgct
    19741 gaagctggcg gatcgcggct acgtgctgga aaacggccat gttgtgctct ccgataccgg
    19801 cgatgcgctg ctggcgaacg aagcagtgag aagtgcgtat ttaggcgggt aaattatcga
    19861 atacccggcg gccacgttct gccgggctct tcgtccgtat acagacttgt ttcaactgac
    19921 cgggaggatt aaaaccagac cacttatttg ccacggaaag gttccctccc atgcattatg
    19981 ccgttgttgt catgcttgaa ttgctttgct gtattcacgc ttaccgtacc ggacaagagc
    20041 ggtactggat ttttatcatt ttctgttttc cggtgattgg ctgcgtggcg tatttcgtca
    20101 tagtgatgct gcctgaaacg ggcgctgacc gtcacgggca tactttgctg atgcgcctgc
    20161 agaacaaact caaccctgag cgccacctgc gcaaattaac cgaagaactg gcgattgccg
    20221 agacgaatca aaaccattat gcgttggcta atgaactggc gcgtctgggg cgttatcacg
    20281 aagctgttcc ccattatcag caggcgttaa gcgggatatt cgcccatgaa gcggcaatga
    20341 tgctaagcct ggcgcaggca cagtttgcta ttcaggagtt cgccgcctgt cagcagacgc
    20401 tggaagatgt gatgcgttat aaccctgatt ttcagtcggc agacgggcat ttgttatttg
    20461 ccagaacgtt agccgcacaa gaaaaatacg ctgatgcgga aagcgagttt gaagtattga
    20521 tttcttatta ccccggcccg caggcgcgta tttactatgc ggaaatgttg gcgaaaatga
    20581 gtcggttacg tgaagccaac gagcagtatg ttgctgtagt tgatacggct aaacgctcaa
    20641 gacctcatta tagaaaacac caccgggaat ggattaagac cgccaatgaa cggctgaagc
    20701 agagtgtggt gcaatgaaaa tcttctttgc cgttagacca gtgtcgcggc aatcttttgt
    20761 taaaattgaa aaatgtagct ctcatcgggc agcaaaactg gacgcagaat ttgctgccat
    20821 ttttgctctt catggtaagg agttctggcg attagctggc cgttaatcgc ctcttttcag
    20881 tgcattctcg ggatatccgt gccccatctc ccattttccc tcacccgtac cgtcaccgcc
    20941 ttgtcatctt tctgacacct tactatctta caaatgtaac aaaaaagtta tttttctgta
    21001 attcgagcat gtcatgttac cccgcgagca taaaacgcgt gaattcgcgc attcggtaca
    21061 acaagagaga taaacgatga aaccgttaca ttatacagct tcagcactgg cgctcggact
    21121 ggcgttaatg gggaatgcac aggcagtgac gaccattccg ttctggcatt ctatggaagg
    21181 ggaactgggt aaagaggtgg attctctggc ccaacgtttt aacgccgaaa acccggatta
    21241 caaaattgta ccgacctata aaggcaacta cgaacagaat ttaagcgcgg ggattgccgc
    21301 atttcgtacc ggcaatgctc cggctatttt gcaggtttat gaagttggca ccgccaccat
    21361 gatggcgtcg aaagccatta aaccggtata tgacgtgttt aaagaggcgg ggattcaatt
    21421 cgatgagtcg cagtttgtgc cgacggtttc aggttactat tccgacagca aaaccggcca
    21481 cttactctcc cagccgttca acagctcgac tcccgttctc tattacaaca aagacgcctt
    21541 caagaaagcg gggttagacc cggaacaacc gccgaaaacc tggcaggatc tggcggacta
    21601 tgccgcaaaa ctaaaagcct ccggcatgaa gtgcggctac gccagcggct ggcagggctg
    21661 gatccaactg gaaaacttta gcgcctggaa cggtctgccg tttgccagca aaaacaacgg
    21721 ctttgacggc acggatgcgg tgctggagtt caacaagccg gagcaggtga aacacatcgc
    21781 catgctcgaa gagatgaaca agaagggcga tttcagctac gtcgggcgta aggatgaatc
    21841 caccgagaag ttctataacg gtgattgcgc gatgaccacc gcctcttccg gttctctcgc
    21901 taatattcgt gagtacgcca aatttaacta tggcgtaggc atgatgcctt acgacgccga
    21961 tgcgaaagat gcaccgcaaa acgccattat cggcggggcc agcctgtggg tgatgcaggg
    22021 caaagataaa gaaacgtata ccggcgtggc gaagttcctc gacttcctcg ctaagccaga
    22081 aaatgctgcc gagtggcatc agaaaactgg ttatctgcca atcaccaaag cggcgtatga
    22141 cttgactcgt gagcagggct tttatgagaa aaacccaggg gcggataccg cgacgcgtca
    22201 gatgctgaat aagccgccgt tgccgttcac caaagggctg cgtctgggca acatgccgca
    22261 gatccgcgtg attgtggatg aagagctcga gagcgtgtgg accggcaaga agacgccaca
    22321 gcaggcgctg gataccgccg ttgagcgtgg gaatcagttg ctgcgccgtt ttgagaaatc
    22381 tacgaagtct taatcagtgt aatgtcggat gcgtttcgct tatctgacct ggcatcgcgt
    22441 gtaagccgga taagcgaagc gcatccggca cagttcagga attaaccgta atgtcatcat
    22501 cccgtccggt gttccgctcg cgctggctgc cttatctgct ggtcgcgccg cagctcatca
    22561 tcaccgttat cttttttatc tggcctgcgg gcgaagcgtt gtggtactcg ctgcaaagcg
    22621 tcgatccgtt tggtttctcc agccagtttg tcggcctgga taacttcgtc gcgctgtttc
    22681 atgacagcta ctatatcgac tccttctgga cgacgataaa attcagcacc tttgtcaccg
    22741 tcagcggttt gctggtatcg ctgttctttg cggcgctggt ggagtacatc gtgcgcggca
    22801 gtcgtttcta tcaaaccttg atgctgctgc cgtatgccgt ggctcccgcc gttgccgccg
    22861 tattgtggat atttttgttt aaccccggtc gcgggctgat cactcatttt ctcgccgagt
    22921 tcggctacga ctggaaccac gcgcaaaaca gcggtcaggc gatgtttctg gtggtgtttg
    22981 cctcggtgtg gaagcaaatc agctacaact ttctgttctt ctatgccgcg ctgcaatcca
    23041 ttccccgttc gttgattgaa gccgcagcca ttgatggcgc aggcccgatt cgccgcttct
    23101 ttaagattgc gctaccgctt atcgctccgg tgagtttctt cctgctggta gtgaacctgg
    23161 tgtatgcctt cttcgacacc ttcccggtga tcgacgccgc cacgtccggt gggccggtgc
    23221 aggccaccac gacgctgatt tataagatct accgtgaagg ctttaccgga ctggatctgg
    23281 cttcgtctgc cgcacagtcg gtggtgttga tgttcctcgt catcgtgctg acggtggtgc
    23341 agttccgcta tgttgaaagc aaggtgcgtt accaatgatt gagaaccgtc cgtggctgac
    23401 gatattcagc cataccatgc tgatcctcgg gatcgcggtg atcctattcc cgctgtacgt
    23461 ggcgtttgtc gcggcgacgc tggataaaca ggccgtctac gccgcgccga tgacgctcat
    23521 ccccggcacg catctgctgg aaaacatcca caacatctgg gtaaacgggg taggcacaaa
    23581 tagtgcgccg ttctggcgga tgttgcttaa cagcttcgtg atggcgttca gcattacgct
    23641 cggcaaaatt accgtctcga tgctctcggc atttgccatt gtctggtttc gttttccgct
    23701 acgtaacctc ttcttctgga tgatttttat caccctgatg ctgccggttg aagtgcgtat
    23761 cttcccgacg gtggaagtca tcgccaacct gaaaatgctc gacagctacg ccggtttaac
    23821 gctgccgctg atggcctcgg cgaccgctac cttcctgttc cgccagttct ttatgacgct
    23881 gccggatgag ctggtggagg ccgcgcggat cgacggcgcg tcaccaatgc gtttcttttg
    23941 cgacatcgtt tttccgttgt ccaaaactaa tctggcggcg ctgtttgtga tcacctttat
    24001 ttacggctgg aaccagtatt tgtggccgtt gttgattatt accgacgtgg atctcggcac
    24061 caccgtggca gggatcaaag ggatgatcgc cactggcgaa ggcaccacag aatggaactc
    24121 agtgatggcg gcgatgttgt taaccctgat cccaccagta gtgattgttt tagtgatgca
    24181 gcgtgccttc gtgcgcggcc tggtcgatag tgagaaataa gatggcagga ctgaaattac
    24241 aggcagtaac caaaagctgg gatggcaaaa cccaggtgat taaaccgctg acccttgatg
    24301 tggcggatgg cgaatttatc gtgatggtcg ggccgtcagg ctgcgggaaa tcgacgctgc
    24361 tgcgcatggt tgccgggctg gagcgggtga cggaaggcga tatctggatc aacgaccagc
    24421 gggtgaccga aatggagccg aaagatcgcg ggattgcgat ggtgttccag aactacgcgc
    24481 tttatccgca tatgagtgtt gaagaaaaca tggcgtgggg gctgaaaatt cgcggcatgg
    24541 gcaagcagca aattgccgag cgcgttaaag aggcggcgcg cattctggaa ctggacggtc
    24601 tgcttaagcg ccgcccgcgc gagctttccg gcggtcagcg tcagcgtgtg gcgatgggcc
    24661 gcgcgattgt gcgcgatccg gcggtgttcc tgtttgatga gccactctct aacctcgatg
    24721 ccaagctgcg cgtacagatg cgtcttgaac tgcaacagct gcaccgtcgc ctgaaaacga
    24781 catcactcta cgttactcac gatcaggttg aggcgatgac cctcgcccag cgagtaatgg
    24841 tgatgaacgg cggcgttgcc gaacagattg gcacaccagt agaagtctac gaaaagcccg
    24901 ccagcctgtt tgtggcgagt tttattggca gcccggcgat gaatctgctg acaggccgcg
    24961 tgaataacga aggcacgcat ttcgaactgg acggcggtat tgtgctaccg ctaaacggtg
    25021 gctaccgtca gtatgccggg cgtaaaatga ctctcggcat tcgcccggaa catattgcgc
    25081 taagctcgca agcagaaggt ggcgtaccgc tggtgatgga cacgctggag atcctcggcg
    25141 cagataacct ggcgcacgga cgatggggcg aacagaagct ggtggtgcga ctggcgcatc
    25201 aggagcgccc gacggcaggc agcacgctgt ggctgcatct ggcggaaaat cagctgcatc
    25261 tttttgatgg tgaaacagga caacgagtat gagtaactgg ccttatcccc gcatcgtcgc
    25321 acatcgtggc ggcggtaagt tggccccgga aaacaccctg gcggcaatcg acgtcggggc
    25381 aaaatacggt cataagatga tcgaatttga cgcgaagttg tcgaaagatg gcgaaatctt
    25441 cctgctccat gacgacaatc tcgaacgtac cagcaacggc tggggcgtcg cgggtgaact
    25501 gaactggcag gatttactac gtgtggatgc aggcagttgg tacggcaaag cgtttaaagg
    25561 tgagccgctg ccgttgcttt cgcaggtggc ggaacgctgt cgcgaacatg ggatgatggc
    25621 gaatatcgaa atcaaaccca ccactggcac cgggccatta acgggcaaaa tggtggcgct
    25681 ggcggcacgc gaactgtggg ccggtatgac gccgccgcta ctgtcatcgt ttgagattga
    25741 tgctttagaa gccgcacaac aggcagtacc agaactgccg cgcggtttgt tgctggatga
    25801 gtggcgcgac gactggcgag aactgaccgc gcggctgggc tgcgtctcta ttcatctcaa
    25861 ttataaattg cttgataaag cacgagtgat gcagttgaaa gacgccggat tacggattct
    25921 ggtttatacc gtcaataaac cccagcgcgc ggcagagttg ctgcgctggg gcgtggattg
    25981 catctgtacc gatgcgattg atgtgattgg tccgaacttt acggcccaat agttttcaac
    26041 ggtatatcag gctgcggcgt gccgggctga ttcagcatgt cgccgtttct cctctccggc
    26101 aacatatgct gctgattcaa cgaactatcc ggattacggt tgctgtttaa catcccgcca
    26161 ttggtgttgg gcagcatttg ctgccgggcg ggatttcgct cccccggctg tgactgcaac
    26221 acccgctgag aattattgtt tatctggttt tctaaatgct gttgttgcaa ctgagtttgc
    26281 gttttcagtt gctgattcag catccctttt tgctggattt gctgcgtttg catctgggtt
    26341 tgcatccgct gctgactggg gatctgatac cccggctggt tagggttgtt cagagtatta
    26401 atgggctgtg caaagccgac aaacggcagg agtgccgtaa gaatcagaag tcgtttcatc
    26461 gcgtatcctc ctctgaagat attctttaag tttactcctt tcctgcaatc atcaggatta
    26521 attcagagtt atataccagg cttagctggg gttgcccctt aatctctgga gaataacgat
    26581 gataaaaccg acgtttttac gccgggtggc cattgctgct ctgttcacag gaagttgttt
    26641 tagtacggta gccgcgccac ctgcgccgtc tcctgtttct tacggcgtgg aggaagatgt
    26701 ctttcatccg gtacgcgcga aacaggggat ggtggcatct gtagatgcta ctgccactca
    26761 ggtgggggtg gatatcctca aggagggcgg gaatgccgtt gatgccgccg tggctgtggg
    26821 ctacgcgctg gcggtaacgc atccgcaggc agggaatctg ggcggtggtg gttttatgct
    26881 gatccgctcg aaaaatggca ataccaccgc tatcgatttc cgcgaaatgg cacccgccaa
    26941 agcgacccgc gatatgttcc ttgatgacca gggcaacccg gacagcaaaa aatcactcac
    27001 ttcgcatctg gcttccggca cgcccggtac ggtcgctggt ttctcgctgg cgttggataa
    27061 atacggcaca atgccgttga acaaagtggt gcagccagcg tttaaactgg cacgcgatgg
    27121 ttttatcgtt aacgacgcgc tggctgacga tctcaaaacc tacgggagcg aagtgctgcc
    27181 gaatcacgaa aacagcaagg ctatcttctg gaaagagggc gagccgctga aaaagggcga
    27241 caagctggtg caggcgaacc tggcaaagag cctggagatg attgccgaaa acggcccgga
    27301 cgaattctac aaaggcacga tagcggaaca gattgcccag gagatgcaga aaaacggtgg
    27361 cctgatcact aaagaagatt tagcggcgta taaagcggtc gaacgcacac cgataagcgg
    27421 cgattatcgc gggtatcagg tttactccat gccaccgcca tcctccggcg ggatccatat
    27481 cgtacaaatc ctcaatattc tggaaaactt cgatatgaag aaatacggtt ttggcagcgc
    27541 cgacgcgatg cagatcatgg cagaagcgga gaaatatgcc tacgctgacc gctcggaata
    27601 tcttggcgac ccggattttg tcaaagtccc gtggcaggcg ctgaccaata aagcctatgc
    27661 caaatctatt gccgagcaaa tcgatatcaa taaagcgaaa ccgtccagcg agattcgccc
    27721 tggcaagctt gcgccttatg agagtaatca aactacccat tactcagtgg tggataaaga
    27781 cggtaacgcc gtggcggtga cctatacgct gaacaccacc ttcggtacgg gtattgtcgc
    27841 gggcgagagc ggtattctgc ttaataacca gatggatgat ttctccgcca aaccgggcgt
    27901 accgaacatt tatgggctgg tgggcggtga tgccaacgcc gtcgggccga acaaacgccc
    27961 gctgtcatcg atgtcaccca ccattgtggt gaaagacggt aaaacctggc tggtcaccgg
    28021 tagccctggc gggagccgga ttattaccac cgtgcttcaa atggtggtga atagcatcga
    28081 ttatggcatg aacgtcgccg aagcgaccaa tgcgccgcgt ttccaccatc agtggttgcc
    28141 ggacgagctg cgtgtcgaaa aagggtttag cccggatacg ctcaagctgc tggaagcaaa
    28201 aggtcagaaa gtggcgctga aagaggcgat gggcagtaca caaagcatta tggttgggcc
    28261 ggacggtgag ctgtatggtg catccgaccc gcgctcggtg gatgatttaa ctgcggggta
    28321 ttaaggttag cggccctctt cgtgggaaga gggctgtttt cttgaacaag ataaattagt
    28381 gtctggattg cttttactat
//
LOCUS       DAZKFU020000036        28268 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZKFU020000036 DAZKFU020000000
VERSION     DAZKFU020000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.036"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..1515
                     /gene="norV"
                     /locus_tag="ACHOQQ_RS06255"
                     /old_locus_tag="pgaptmp_001251"
     CDS             76..1515
                     /gene="norV"
                     /locus_tag="ACHOQQ_RS06255"
                     /old_locus_tag="pgaptmp_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGU4469527.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            1512..2645
                     /gene="norW"
                     /locus_tag="ACHOQQ_RS06260"
                     /old_locus_tag="pgaptmp_001252"
     CDS             1512..2645
                     /gene="norW"
                     /locus_tag="ACHOQQ_RS06260"
                     /old_locus_tag="pgaptmp_001252"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGU4469528.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVMNIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(2871..5123)
                     /gene="hypF"
                     /locus_tag="ACHOQQ_RS06265"
                     /old_locus_tag="pgaptmp_001253"
     CDS             complement(2871..5123)
                     /gene="hypF"
                     /locus_tag="ACHOQQ_RS06265"
                     /old_locus_tag="pgaptmp_001253"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGU4469529.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRVFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLTTFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(5276..5803)
                     /gene="hydN"
                     /locus_tag="ACHOQQ_RS06270"
                     /old_locus_tag="pgaptmp_001254"
     CDS             complement(5276..5803)
                     /gene="hydN"
                     /locus_tag="ACHOQQ_RS06270"
                     /old_locus_tag="pgaptmp_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGU4469530.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(5953..6963)
                     /gene="ascG"
                     /locus_tag="ACHOQQ_RS06275"
                     /old_locus_tag="pgaptmp_001255"
     CDS             complement(5953..6963)
                     /gene="ascG"
                     /locus_tag="ACHOQQ_RS06275"
                     /old_locus_tag="pgaptmp_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGU4469531.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVMGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            7223..8680
                     /gene="ascF"
                     /locus_tag="ACHOQQ_RS06280"
                     /old_locus_tag="pgaptmp_001256"
     CDS             7223..8680
                     /gene="ascF"
                     /locus_tag="ACHOQQ_RS06280"
                     /old_locus_tag="pgaptmp_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGU4469532.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEIVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAISGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            8689..10113
                     /gene="ascB"
                     /locus_tag="ACHOQQ_RS06285"
                     /old_locus_tag="pgaptmp_001257"
     CDS             8689..10113
                     /gene="ascB"
                     /locus_tag="ACHOQQ_RS06285"
                     /old_locus_tag="pgaptmp_001257"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGU4469533.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(10272..10742)
                     /gene="hycI"
                     /locus_tag="ACHOQQ_RS06290"
                     /old_locus_tag="pgaptmp_001258"
     CDS             complement(10272..10742)
                     /gene="hycI"
                     /locus_tag="ACHOQQ_RS06290"
                     /old_locus_tag="pgaptmp_001258"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGU4469534.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(10735..11145)
                     /gene="hycH"
                     /locus_tag="ACHOQQ_RS06295"
                     /old_locus_tag="pgaptmp_001259"
     CDS             complement(10735..11145)
                     /gene="hycH"
                     /locus_tag="ACHOQQ_RS06295"
                     /old_locus_tag="pgaptmp_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGU4469535.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(11142..11909)
                     /gene="hycG"
                     /locus_tag="ACHOQQ_RS06300"
                     /old_locus_tag="pgaptmp_001260"
     CDS             complement(11142..11909)
                     /gene="hycG"
                     /locus_tag="ACHOQQ_RS06300"
                     /old_locus_tag="pgaptmp_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGU4469536.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGNMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(11909..12451)
                     /gene="hycF"
                     /locus_tag="ACHOQQ_RS06305"
                     /old_locus_tag="pgaptmp_001261"
     CDS             complement(11909..12451)
                     /gene="hycF"
                     /locus_tag="ACHOQQ_RS06305"
                     /old_locus_tag="pgaptmp_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGU4469537.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(12461..14170)
                     /gene="hycE"
                     /locus_tag="ACHOQQ_RS06310"
                     /old_locus_tag="pgaptmp_001262"
     CDS             complement(12461..14170)
                     /gene="hycE"
                     /locus_tag="ACHOQQ_RS06310"
                     /old_locus_tag="pgaptmp_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGU4469538.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(14188..15111)
                     /gene="hycD"
                     /locus_tag="ACHOQQ_RS06315"
                     /old_locus_tag="pgaptmp_001263"
     CDS             complement(14188..15111)
                     /gene="hycD"
                     /locus_tag="ACHOQQ_RS06315"
                     /old_locus_tag="pgaptmp_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGU4469539.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(15114..16940)
                     /gene="hycC"
                     /locus_tag="ACHOQQ_RS06320"
                     /old_locus_tag="pgaptmp_001264"
     CDS             complement(15114..16940)
                     /gene="hycC"
                     /locus_tag="ACHOQQ_RS06320"
                     /old_locus_tag="pgaptmp_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGU4469540.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(16937..17548)
                     /gene="hycB"
                     /locus_tag="ACHOQQ_RS06325"
                     /old_locus_tag="pgaptmp_001265"
     CDS             complement(16937..17548)
                     /gene="hycB"
                     /locus_tag="ACHOQQ_RS06325"
                     /old_locus_tag="pgaptmp_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGU4469541.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(17673..18134)
                     /gene="hycA"
                     /locus_tag="ACHOQQ_RS06330"
                     /old_locus_tag="pgaptmp_001266"
     CDS             complement(17673..18134)
                     /gene="hycA"
                     /locus_tag="ACHOQQ_RS06330"
                     /old_locus_tag="pgaptmp_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGU4469542.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            18346..18696
                     /gene="hypA"
                     /locus_tag="ACHOQQ_RS06335"
                     /old_locus_tag="pgaptmp_001267"
     CDS             18346..18696
                     /gene="hypA"
                     /locus_tag="ACHOQQ_RS06335"
                     /old_locus_tag="pgaptmp_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4469543.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            18700..19572
                     /gene="hypB"
                     /locus_tag="ACHOQQ_RS06340"
                     /old_locus_tag="pgaptmp_001268"
     CDS             18700..19572
                     /gene="hypB"
                     /locus_tag="ACHOQQ_RS06340"
                     /old_locus_tag="pgaptmp_001268"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGU4469544.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     TPEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            19563..19835
                     /gene="hypC"
                     /locus_tag="ACHOQQ_RS06345"
                     /old_locus_tag="pgaptmp_001269"
     CDS             19563..19835
                     /gene="hypC"
                     /locus_tag="ACHOQQ_RS06345"
                     /old_locus_tag="pgaptmp_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGU4469545.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            19835..20956
                     /gene="hypD"
                     /locus_tag="ACHOQQ_RS06350"
                     /old_locus_tag="pgaptmp_001270"
     CDS             19835..20956
                     /gene="hypD"
                     /locus_tag="ACHOQQ_RS06350"
                     /old_locus_tag="pgaptmp_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGU4469546.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            20953..21963
                     /gene="hypE"
                     /locus_tag="ACHOQQ_RS06355"
                     /old_locus_tag="pgaptmp_001271"
     CDS             20953..21963
                     /gene="hypE"
                     /locus_tag="ACHOQQ_RS06355"
                     /old_locus_tag="pgaptmp_001271"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGU4469547.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLIAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLASLYGVKRTLDLPHAEPLPR
                     IC"
     gene            22037..24115
                     /gene="flhA"
                     /locus_tag="ACHOQQ_RS06360"
                     /old_locus_tag="pgaptmp_001272"
     CDS             22037..24115
                     /gene="flhA"
                     /locus_tag="ACHOQQ_RS06360"
                     /old_locus_tag="pgaptmp_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGU4469548.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            24160..24588
                     /locus_tag="ACHOQQ_RS06365"
                     /old_locus_tag="pgaptmp_001273"
     CDS             24160..24588
                     /locus_tag="ACHOQQ_RS06365"
                     /old_locus_tag="pgaptmp_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HGU4469549.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(24627..24971)
                     /gene="ygbA"
                     /locus_tag="ACHOQQ_RS06370"
                     /old_locus_tag="pgaptmp_001274"
     CDS             complement(24627..24971)
                     /gene="ygbA"
                     /locus_tag="ACHOQQ_RS06370"
                     /old_locus_tag="pgaptmp_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGU4469550.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(25048..25182)
                     /locus_tag="ACHOQQ_RS06375"
                     /old_locus_tag="pgaptmp_001275"
                     /pseudo
     CDS             complement(25048..25182)
                     /locus_tag="ACHOQQ_RS06375"
                     /old_locus_tag="pgaptmp_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25258..27819
                     /gene="mutS"
                     /locus_tag="ACHOQQ_RS06380"
                     /old_locus_tag="pgaptmp_001276"
     CDS             25258..27819
                     /gene="mutS"
                     /locus_tag="ACHOQQ_RS06380"
                     /old_locus_tag="pgaptmp_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGU4469551.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIAPGYNEELDEWRALADGATDYLERL
                     EIRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            27925..>28268
                     /locus_tag="ACHOQQ_RS06385"
                     /old_locus_tag="pgaptmp_001277"
     CDS             27925..>28268
                     /locus_tag="ACHOQQ_RS06385"
                     /old_locus_tag="pgaptmp_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGU4469552.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FELNDMEQKEAIG"
BASE COUNT         6688 a         7752 c         7621 g         6207 t
ORIGIN      
        1 tagataaaaa actggcacgc aatctgcaat tagcaagaca tctttttaga acacgctgaa
       61 taaattgagg ttgctatgtc tattgtggtg aaaaataaca ttcattgggt gggtcaacgt
      121 gactgggaag tgcgtgattt tcacggcacg gaatataaaa cgctgcgcgg cagcagctac
      181 aacagctacc tcatccgcga agaaaaaaac gtgctgatcg acaccgtcga ccataaattc
      241 agccgcgaat ttgtgcagaa cctgcgtaat gaaatcgatc tggcggatat cgattacatc
      301 gtgattaacc atgcagaaga ggaccacgcc ggggcgctga ccgaactgat ggcacaaatt
      361 cccgatacgc cgatctactg tacagccaac gctatcgact cgataaatgg tcatcaccat
      421 catccggagt ggaattttaa tgtggtgaaa actggcgaca cgctggatat cggcaacggc
      481 aaacagctca tttttgtcga aacaccaatg ctgcactggc cggacagcat gatgacttac
      541 ctgacaggcg acgcggtgct gttcagtaac gatgctttcg gtcagcacta ctgcgacgag
      601 catctgttca acgatgaagt ggatcagacg gagcttttcg agcagtgcca gcgttactac
      661 gccaatatcc tgacgccgtt cagccgcctg gtaacgccga aaattaccga gatcctgggc
      721 tttaacttac cagtcgatat gatagcaacc tcccacggcg tggtctggcg cgataacccg
      781 acgcaaattg tcgagctgta cctgaagtgg gccgctgatt atcaggaaga cagaatcacc
      841 attttctacg acaccatgtc gaataacacc cgcatgatgg ctgacgctat cgcccagggg
      901 attgcggaaa ccgacccacg cgtggcggtg aaaattttca acgtcgcccg aagcgataaa
      961 aacgaaatcc tgaccaatgt cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg
     1021 aataacgtga tgatgccgaa aatcgccggg ctggtggagg agatgaccgg attacgcttc
     1081 cgtaacaaac gcgccagtgc tttcggctct cacggctgga gcggcggtgc ggtggatcgt
     1141 ctttccacgc gcctgcagga tgcgggtttc gaaatgtcgc ttagcctgaa agcgaaatgg
     1201 cgaccagacc aggacgctct ggagttatgc cgtgaacacg gtcgcgaaat cgcccgtcag
     1261 tgggcgctcg cgccgctgcc gcagagcacg gtgaatacgg tagttaaaga agaaacctct
     1321 gccaccacga cggctgacct cggcccacgg atgcagtgca gcgtctgcca gtggatttac
     1381 gatccggcaa aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg gagtgaagtc
     1441 ccggataact tcctctgccc ggaatgctcc ctcggcaaag acgtctttga cgaactggca
     1501 tcggaggcaa aatgagcaac ggcattgtga tcattggttc gggctttgcc gcccgccaac
     1561 tggtgaaaaa tattcgcaaa caggacgcca ctattccatt aaccctgatt gccgccgaca
     1621 gcatggatga gtacaacaaa ccggatctca gccatgttat cagccagggg caacgtgccg
     1681 atgaccttac ccgccagacg gcgggtgaat ttgccgagca gtttaatctg cgcctgtttc
     1741 cgcacacctg ggtgatgaat atcgatgccg aagcccgtgt ggtgaaaagc cagaataatc
     1801 agtggcaata cgacaagctg gtgctggcaa ctggtgccag cgcctttgtc ccgcctgtgc
     1861 ctgggcgtga gttaatgctg acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc
     1921 aactgcggga tgcccgacgc gtgttgattg ttggcggtgg tttgattggt agcgaactgg
     1981 cgatggattt ttgtcgtgca ggcaaagcgg tcacgctaat cgacaacgct gccagtattc
     2041 tggcgtcgtt aatgccacca gaagtaagca gccgcttgca gcatcggttg acggagatgg
     2101 gcgttcatct gctgttgaaa tctcagttgc aggggctgga aaaaacggat tctggcattc
     2161 tggcaacgct ggaccgccag cgtagcatcg aagtggatgc ggtaattgcc gccaccggac
     2221 tgcgcccgga aaccgccctg gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg
     2281 tcgatagtta tctgcaaacc agtaatgccg atatttacgc gctgggcgat tgcgcggaaa
     2341 ttaacggtca ggtactgccg ttcctgcagc cgattcaact tagcgcgatg gtgctggcaa
     2401 aaaatcttct cggcaataac acgccgctga aactaccggc gatgctggtg aaaatcaaaa
     2461 cgccggaatt accgctgcat ctggcaggcg aaacccagcg tcaggattta cgctggcaga
     2521 ttaataccga acgccaggga atggtggcgc gcggtgttga cgatgcagac caacttcgcg
     2581 cctttgtggt cagtgaggat cggatgaaag aggcatttgg attgttgaaa acattgccgg
     2641 tgtaggcggg ctactgtgcc caaaatgtcg gatgcgacgc tgacgcgcct tatccgacct
     2701 acggggagcg catgtgttgg tcacataatg cgttcatgtc gcatccgcct actgtgccca
     2761 aaatgtcgga tgcgacgctg gcgcgtctta tccgacctac ggggacgcat gtgtaggccg
     2821 gataaggcgt ttacgccgca tccggcaatg gtgtccaaat gcaacacgat ttatccgttc
     2881 tggacttcac ccgctaacca acgcgccgca gcaataaccc cctgccccag agacaaaccg
     2941 ccatcacccg ccggtaaact ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc
     3001 agacgtgcac gcagcaaacg gttatgaata accccgccgc taaataccag cgtagtgata
     3061 ccacgcatcg tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga
     3121 aacgcccacg cgcgttgatt aaccggtgcc tgccagttca gccactgctg ccagaaagtg
     3181 gtgagatcca gttgattgtc caccagcggc attgtcaccg gatgcgtcac tccgtggcat
     3241 gaggctgcga gcgcctccag agcacaagcc gcttcacctt cataacttaa cgtggctggc
     3301 gcacagccca gtgccgccgc cactgcatcg aacacccgcc cacacgatga cgccagcggc
     3361 gcgttaattc cacgctcaat ggcccgcgcc agaacgctcc agttttgctg ttgtacactt
     3421 gccgtttcag ggtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggtta
     3481 cgccacggct gcttcgccgc caaatcgcca cccggaagcg ccactgcagg caagccgccc
     3541 aggtgctcac attcgcgata gttcacccgc aggcactcgc cgccccacaa agcgccgttc
     3601 tcccccatac cgataccgtc gagcgtcaaa gcaatgacat caccgccctc cagtggccag
     3661 tgatgttctg ccagacacgc cgccgcgtgg gcatgatgat gcagcaccgt ttgcgtcggc
     3721 agattcattt cgcgcgccca ctggctggag acatagcccg gatgtgcgtc atgcacaacg
     3781 tattgcgggg tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc
     3841 tggatgccat cgtcacttaa atcgcccaga tgctggctca acaccgcttg ttcaccgcgc
     3901 accaggcaga aggtattttt cagatccgcg ccgagacaca gcacaggcgg aacattttta
     3961 aagcccggag gcaaagccag cgcatccggc acataccccc gcgaacggcg tagcatttcg
     4021 ccgctttcgc gcaccaccga atcatccatc cgctgcacga tgtcgcggtt atgtatcaag
     4081 aatccgtcgg caatgccctg caaatctgcc agcgcctgtt cgttgctgat agccggtggt
     4141 ttaccgctca ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa
     4201 tgctggagcg ggttcgcagg caacattacc ccgacttcgt taaggtcagg ggcgatatca
     4261 tcacaaagct caggtacgta ttttttatcc accagcacaa tcggcgcggc gggcgtggta
     4321 agcaactggc gcgcagcgtc tggtaaaccg tcagccactg gcaacatgac cgccagcggt
     4381 ttcgccgggc gatgtttgcg cgcccgcagt gtcgccaccg cgttactgtt acgtgcatcg
     4441 caggcaagat gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt
     4501 gcgatagccg cctgtaatgc cgcctcttgt tctgcatgtt caccatgact tacccattca
     4561 agatgcgggc cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg
     4621 tcgcggtact ctttatcaca ggctggacac agcggaaacg ccgccatcac ggtaaacggg
     4681 cgatcgtaag gcatggcgcg aataatggtg aaacgcggac cgcagtgggt acagttgata
     4741 aacggataac gccagcgtcg ctcgccaggg gtattcattt cggcaaggca ggcagggcaa
     4801 gtggcggcat cgggaacaat ttgcgtattc atggcgccgc ccgcgctctg gcggatagtg
     4861 aactcggtgg gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc
     4921 agcggcgggc agtgctgatg caattgaaca agaaacgttt ccgggtcttc cagcagccgg
     4981 acttctacgc catcgccgtc attacagaca tcgccgtgaa gatttaattg ctgtgccagc
     5041 tgccagacaa acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca
     5101 ccgcaagatg tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgtc
     5161 cagccggaca cgacagcgtc gcatccggta gtcacaggtc ggcgatactg cccgcttcgt
     5221 attctacgaa tatttccggg aattcctttg atgccagaac agttctgtaa gatttttaga
     5281 acatcagcgc cgtgcggcga cgtttttctg cgctcagttg ttcaagttta ttacgatcga
     5341 cacaaatcag cgcatgagtc gggcaagccg ccatacacgc cggaccgtct tcacgatggt
     5401 tgcacaggtc gcatttattg gcttcggctt tgtcagcccg tacattcagc cctgcgccgc
     5461 tgttgcgaat caccggacgt accaccactt ccatcgcgcc atacgggcaa gccacaacgc
     5521 aggttttgca accaatgcaa cgttcctgca tcacatgaac aaacccttta tcacggctga
     5581 tagcaccatt cgggcagacg ttagcgcacg gtgcatcttc acactgacgg caaactgtcg
     5641 ccgtggaaat gttcacacct ttaatgacat ggatacgcgg taaaaatgtt tccggggtca
     5701 gcgatgcaca gtcctgattt tcctgatgag aaaccacgca cgccacttca caggtacggc
     5761 aaccaataca tttactcgcg tcagcaatga tgaaacggtt catcaaattc tccagcaatg
     5821 acagttaatg cgccgataca ttcacaaatc atgccagttt ttaattcact gttatttaag
     5881 gaaattaatt tctgtaatgc aggaaaaaaa gatgtcatcg acactagtga cgatgacatg
     5941 tgatgacaat gtttatcgcg aaagagcaat gagtgagtcg cggcggatca gttttccgct
     6001 gaaggttttc ggcggtgaga aatccccgcc atcgagcata aaaatcagcc gtccaataat
     6061 ttcctgaatc atctctgtta ccggaatttt tacgctggag agcgccggaa cggtgtaggg
     6121 ggcaatagcg atatcatcga atcccataac tgacacctgc tctggcaccg ctacgccgcg
     6181 ctcgtgtaac gctttcatcg cacctatcgc catatcgtcg ttactggcaa ctaacgcgct
     6241 aaatttagcc ccacgttcga gcaacatttc taccccttcg gccccgctgg caggcgtcca
     6301 tttaccgtta gcgataagtt tttcattgag cgcaatacca tgctgcgaca gcgcgtcttt
     6361 atacccggca agacgttcaa tgctggtggg ggaatccatc gagccggtaa ggaaagcaat
     6421 ctcctgatgt ccggcgttta tcaactctgc cacggcgtta aagctggtct gtttatgatc
     6481 gcaccagacg ctatggctgc tgtttttgcg caggcggcga ttgagcacca ttatcggctg
     6541 actgtgcgcg tcaatgatgt catcgatctc atccacgctt aaaaaacgcg ggtaaatcat
     6601 aatcgcgtcg cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt cttcggcgct
     6661 gtgtttacca tctgccaata gtaactgtcg ccctttctct tccgccattc gcgcggcatg
     6721 aaacagtaat tcactaaaat aaatgccgtg gtaaagcgtg ttggtcacta ccagccccag
     6781 cgtctgagta ctcttcgccg acagattacg cgccagcaag tttggacggt aaccgctctc
     6841 ttctaccgcc tgaaacacgc gatctttagt ctcctggctg acgtagccat tacctgaaag
     6901 cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca gcatcgtcgt
     6961 catcattttc atccctttac acgcaatcaa cgcagtgtac tgcaccgttt gccgattgtc
     7021 cttgcgcaat cggcgggaaa aatattcagg tgaccggttt cacaaatata aaaaatgaac
     7081 aattaactct cttgcttatt tggtgacaac tattcatgat tttgtgaaac cggtttctta
     7141 attccgtttc agcatcggca tttttccgtc acgtcgactg ataacaacca catctaccct
     7201 actgataaca ggataaaatc cgatggccaa aaattatgcg gcgctggcac gctcggtgat
     7261 agcggcactg ggcggcgttg ataacatctc ggcagtcacg cactgtatga cgcgtttgcg
     7321 ctttgttatc aaagatgatg cgcttatcga cagcccgacg ttaaaaacca tccccggcgt
     7381 gctcggcgtg gtacgcagtg acaaccagtg tcaggtgatt atcggcaata ccgtttcaca
     7441 agcctttcag gaaatcgtca gcctgctgcc gggagatatg cagcccgcac agcccgtagg
     7501 taaacccaaa ctcacgctac gtcgcattgg tgcggggatc ctcgatgcgc tgatcggcac
     7561 catgtcaccg ctgatcccgg cgattatcgg cggatcgatg gtcaaactgc tggcgatgat
     7621 cctcgagatg agcggcgtgc tgacaaaagg atcgccgacc ttaaccattc tgaatgtgat
     7681 tggtgacggt gctttcttct tcctgccgct aatggtcgcg gcatctgccg ccatcaaatt
     7741 taaaaccaat atgtcgctgg cgattgccat ctcgggtgtg ctggtacatc cgagttttat
     7801 tgaactgatg gcgaaagcgg cccaggggga acatgtcgaa tttgccctga tcccggtcac
     7861 cgcggtgaaa tacacctaca cggtgatccc ggcgctggtg atgacctggt gcctgtcata
     7921 tatcgaacgc tgggtggatc gcattacacc cgcggtgacc aaaaacttcc tcaagccaat
     7981 gctgattgtg ttgattgccg caccgctggc aatcctgctg attggcccga ttggtatctg
     8041 gatcggtagc gccatttcgg cgctggttta caccattcat ggttatctgg gctggctttc
     8101 agtcgccatt atgggcgcgc tgtggcctct gctggtaatg accgggatgc accgcgtctt
     8161 tacgccaacc atcattcaga ccattgccga aaccggcaaa gaagggatgg tcatgccgtc
     8221 agagatcggc gctaacctgt cgctgggcgg ttcatcactg gcggtggcgt ggaaaacgaa
     8281 aaacccggaa ctgcgccaga ctgcgctggc tgcggcggca tcagccatta tggcggggat
     8341 ttccgaaccg gcgttatacg gcgtggcgat ccgcctgaaa cgtccgctta tcgccagtct
     8401 tatcagcggt tttatttgcg gcgcggttgc cggtatggcg gggcttgcca gccactcaat
     8461 ggcagcgccg gggctattta ccagcgtgca gttcttcgat ccggcgaatc caatgagcat
     8521 cgtctgggtg ttcgcggtca tggcgctggc ggtggtgctg tcgtttatcc tcacactgtt
     8581 gctcggcttt gaggatattc ctgttgagga agcggctgcc gaggcgcgaa agcatcagag
     8641 cgtacaaccg accgtcgcca aagaagtaag tcttaattga ggatgaaaat gtcagtattt
     8701 ccagaaggtt ttttatgggg cggcgcgctt gccgccaacc agtctgaagg tgcgttccgt
     8761 gaaggtggca aaggactgac aacggtcgat atgatcccac acggcgagca tcgaatggcg
     8821 gtgaaactgg ggctggaaaa acgttttcag ttgcgcgatg acgagtttta tcccagccat
     8881 gaggcgacgg gtttttatca tcgttataaa gaagatatcg ccctgatggc agagatggga
     8941 ttcaaggttt tccgtacctc aattgcctgg agccgtctct ttccgcaggg cgatgaaatc
     9001 acgcccaatc agcagggcat tgctttttat cgttctgtct ttgaagagtg taaaaagtac
     9061 ggtatcgaac cgctggtcac gttgtgccac ttcgatgtgc cgatgcatct ggtcaccgaa
     9121 tatggctcct ggcgtaaccg caagctggtg gagtttttca gccgctacgc ccggacctgc
     9181 tttgaagcat ttgatggtct ggtgaaatac tggctaacct tcaatgaaat caacattatg
     9241 ttgcatagcc cgttctccgg cgcgggtctg gtgtttgaag aaggtgaaaa tcaggatcag
     9301 gtgaaatatc aggccgcgca tcaccagctg gttgccagtg cgctagccac caaaatcgcc
     9361 catgaggtta acccgcaaaa tcaggtgggc tgtatgctgg cgggcggcaa cttctaccct
     9421 tacagttgca agccggaaga tgtctgggcg gcgctggaga aagatcggga aaacctgttt
     9481 tttatcgatg tgcaggcgcg gggcacgtat ccggcttact ctgcccgcgt attccgcgaa
     9541 aaaggggtaa ccatcgacaa agcaccgggc gatgatgaaa tcctgaaaaa caccgtcgat
     9601 tttgtctctt tcagctatta cgcctcgcgc tgcgcctcgg cggagatgaa cgccaacaac
     9661 agcagtgcgg cgaacgtggt gaaatcgctg cgtaacccgt atctgcaagt gagcgactgg
     9721 ggctggggaa ttgatccact cggtctgcgt atcaccatga acatgatgta cgaccgctat
     9781 cagaagccgc tgtttctggt ggaaaacggc ctcggcgcaa aagatgaact tgctgccaat
     9841 ggcgagatta acgatgacta tcgcatcagc tatttacgcg aacatatccg cgcaatgggc
     9901 gaagcgattg cagacggcat tccgctgatg ggctacacca cctggggctg tattgattta
     9961 gtttccgcct ctacgggtga aatgagcaaa cgctacggtt ttgtctttgt tgaccgtgac
    10021 gacgcaggca acggcacgct gacgcgcaca cgtaagaaat cgttctggtg gtataaaaaa
    10081 gtgattgcca gtaatggtga agatttagag taagtaaccg tgccggatgc ggcgtgaacg
    10141 ccttatccgg cctacatagt tcgtgcgaca tgtaggcccg ataagcgcag cgcatcgggc
    10201 aatttagcat ttggcatcag agctggttca ccccatcaag aacatccctg tcctgattcc
    10261 ttattgaaaa actactcttc ttccaccgct aactgcgcga agccgccgtt cccttcccag
    10321 ccttccagtc gttgataaac ggtttctacc gcatctttaa tcggctgggt catcgggtag
    10381 taaaagccga cgatatccgg ctggatgccg aggaaaatca cttcgccaat atcttctttc
    10441 aactgatcga taaggtagtt caacggcatg ttatgggtag tcatcataaa catctcggcg
    10501 atatcatccg ggtcgatgat gcggatttcg ccggggttta gccccatatc agtagcgtca
    10561 acaatcagta accgcgtagg gcgcagttca cggatggcga cgatgtcgtt ttccggtgcg
    10621 ctaccgccgt caatcaccac ccagttacct ttcggcgcgg cggcgcactt ttccgccagc
    10681 agcggacctg cgccatcatc gcccatcatg ctattgccaa cacagagtaa aacgtcagtc
    10741 acgcagtctc ctcaccatca aatagatggc gttttcctga tgaatatcat gcagcatact
    10801 taacagcatt gtactccatt cctgctgctg cgaagtttgc gctgcccggg ctttatcgaa
    10861 tgcattggcc agcatcggca catggttaat gtcgatgacg atctcaccat atttcggcac
    10921 accttccatt ttgcggcggg cttcgctgcc cgcctccaga gtggcaatcc atgccagata
    10981 ttcatcccac gggcaggtga gcgccgcttc caggcaatcg ataaccccaa ggtggtgacc
    11041 aatcgccagg ctgtaataga ccacctgctg cgcctcggcg ggcgtggcat cgttctcatc
    11101 aataaattta cggctcagtt gactgaacac caccttttca ctcatcggat acgcgcctct
    11161 tctacaacat gattcagatg gctgacaatc tcgttcagac gcggatcgtt ttccgcttcc
    11221 agccagcgtg caacctgttc ttcgccctgc cctaactgtg taaggtaatc atcggcaatc
    11281 tgacgaccgt aacgataacc cgccagtcga cgtgcttcgc gatccacttt cacacgcagc
    11341 ggctgcacca tattgccatg cagaatttct gccggttgtt catcctgctc acccggccca
    11401 cgggcgtgaa ttttctgctc cagcaggccg agcgccattg caaagccgta cagcgtggcg
    11461 gcaggcgttg gcgggcagcc agggatataa acatccaccg ggacgatttt atccgtaccg
    11521 ccccacacgc agtagagatc gtggaagatc ccgccgctgt taccgcaggc accgtaggag
    11581 atacaaattt tcgggtccgg cgcggactgc cacgcacgca gcgcagggga tcgcattgca
    11641 cgggtgaccg cgccggtaaa cagtaaaata tccgcatgac gcggtgaagg aacgacttta
    11701 atgccgaagc gttctgcatc gaacagcggc gacaaagtgg cgaaaatttc gatttcgcag
    11761 ccgttgcagc cgccgcagtc cacgcggtaa acataggcag aacgtttgat ttttttcagt
    11821 aacgacgcct tcatgctggc gatggattca tccaccgtca tcgggaccgg aatgccgttg
    11881 gcgtcacggg ggcctaataa attgctcatc agatggcctc tttcatatgg cgagtcagtt
    11941 caatacggtc ggacggcacc aggcatttct ggcgcttaca ttccgggcaa gtctcaaagc
    12001 tttcgcggtg gttttccgcg cggctgtcgc cgttgtgctt aagcagcgca atggcgtagt
    12061 cgatctcttt ctggacggcg aaaggacgat tgcagacgcg acagttgcac agcgcgaagc
    12121 gggactgttg caggaagtct tctttcttcc acaccgccag ttcgtactct tgcgacagct
    12181 taatcgccgc cgtcgggcag acttcttcgc agcgtccgca gaagatgcaa cgcccgagat
    12241 taaactccca ggccagctct cctgtggcga ggtcagtttc aaccgttaag gcgtttgacg
    12301 ggcaggcatt gacgcaggcc gcgcagccga tgcactgctg cgggttctgc tctggcttac
    12361 cacggaagtt tttatcaacc gcaatcggct ccagcggata agacgaggtc gccgtgccgg
    12421 ttttgatgac ttttttgata aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt
    12481 tacgctcaat gctgtaacgc tcgagttctt tgtacggcac cactttgctc ttcttcttac
    12541 gcacatcgac cacggtcatg cggtcggtac aggagtagca agggtcgagg ctaccgataa
    12601 tcagcggcgc atcggaaacg gtattgccac gcagcatgta gcgcagggtc ggccagttcg
    12661 cgtaagtcgc ggcacggcag cgccagcggt acagcttctg gttgtcgccg gtcatgctcc
    12721 agtggatatc atcgccgcgc ggcgcttcgg caaagcccag cgcaaagcgg tgcggaatgt
    12781 aggtaaagcc ttccaccatc agtgggccac ccggcaggtt atccagaccg tagtcgatca
    12841 tgttcagcgc ggtatagact tcgttgatac gcactttcag acgggaaata acgtcgcagc
    12901 cctgctcgct gtggacttcc attggcagca ggccataacc aacaaacggg tgatcggcgc
    12961 gagtatcgcg agcgtgaccg ctggcacgga ccatcgggcc gacgttactg aagtcgcgag
    13021 cgatttccgg gtccagacga ccaatgccga cagtgcgttg ttccatgttc ggtgtgctca
    13081 acagcacatc caccagctcc tgcacttcac gacgcatctg ttgcgccagc tggcgggtct
    13141 ggatcatgtc atctttcagc agatcgcgac gaatcccgcc gatcaggttc aggccgtagg
    13201 ttttacgcgc accggtaagg atctctgcca ttttcatgga ggtttcacgc acgcggaaga
    13261 actgcataaa gccggagtca aagccggtaa agtggcaggc aagaccgagg ttgagcagat
    13321 gcgagtgcag gcgttccacc tccagcagaa tggcgcggat catctgcgca cgttctggca
    13381 cctgaatacc catcgcgttt tccaccgacg tggtgtaggc ggtgctgtga gcaaagccgc
    13441 agatcccgca cacacggtca gagaggaagg tcacttcgtt gtaacccata cgggtttccg
    13501 ccagtttttc catgccgcga tgaacgtaga acagacggta gtcggcgtcg ataatgtttt
    13561 cgccatcgac gaacagacgg aagtgacccg gttcgtcaga agtgacgtgc agcggaccaa
    13621 tcggcacgac gttgtttttc ttgtcgccca gttcgttgat gaactcgtag gtttcagcat
    13681 cggtggtcgg tgccggacgt tgacgataat ccatgctgtc tttacgcagc ggataaagtt
    13741 catccggcca gtcatccggc agcaccagac ggcgttcatc cggcagccct accggaatca
    13801 aaccgtacat atcgcgcact tcgcgctcgc cccacaccgc cgccggaacg cgcggcgtca
    13861 cggacggata ctccggtttg ttggcgtcaa cttcgacgcg aaccgtaatc caacacttag
    13921 tgcccttctc catcgacagc acgtagtaaa cggcgtaatg accattcagt ttgcgttcgt
    13981 cgttaccaaa cagcaccgac agccagccac cctgtttgta gtagagaaac tccaccactt
    14041 ccggcagata gttcaccttc acggtcacgg tcagctgatc tttggtctgc caggcgtggt
    14101 ccagcacgac gcccggaaat gcctcattca gcgcggcgag ataatgttga cctaattttt
    14161 cttcagacat gctcaaactc tctttaatca cgccgccagc aaggagacga acgctaaaaa
    14221 tgcaaagcca aacccagccc aggtaatgcg cggagtaata tcaagacgca gacgggccat
    14281 gctgttttcg aacagcgcga taaccaggac gccgaccacc agtttgacga tggcaatcac
    14341 cagcgccagc agcagtccac cagcggtgaa ggtttccatt tgtccccacg gaataaacac
    14401 cccgacgaac atctgcagca ccaccagctg tttcaggctg ataccccatt tcatgacgcc
    14461 aaagccgctg ccgctatact cagagagcgg accttcctgc aactcctgct cggcttccgc
    14521 caggtcgaac ggcagtttgc ccatttcgat aaaagtggcg aacgcacagg cacaaagcgc
    14581 cagcaccagc gggatgctct ggctcagcgg ccagtgataa acggtgtcgg tgatgttgct
    14641 gatgttggtg gaaccggcaa cctgtgcggc aacccacaga ccaagcagca acatcggttc
    14701 gaccagcacg ccaagcatcg cttcacggct cgcgccgata gcggtaaacg ggctaccggt
    14761 atccagacca gaaatggcaa agaagaaacg cgcgatggca aagagataca gtaaggtgat
    14821 caaatcaccc agttgcggca gcggagaacc aacggtcacc accggcagcg cagtagcgat
    14881 agtcagcatg acgcccacca tcacatacgg cgtcaggcgg aacacccagc cggaggcatc
    14941 cgggccaacg ctctggcgac ccagcagttt gataatgtcg cgatactcct gcaacacgcc
    15001 cggcccgcga cggttatgca agcgggcgcg cgccacgcgg gttataccgg agagcagcgg
    15061 cgcaacggca aatagcacca gcgcctgaat taacggatat aaaacactca ttctcaggct
    15121 cctcgtgaaa caataatcac caccagcacc gccagttcaa ccagcgccat ccggcggaac
    15181 agcaacgcac tcccctcgca ctgccagccc ggcaccagag acaccggatt cagccatttg
    15241 cgtagtttca gcaccggcgc aaacgcctgt ttcaccggca tggcaaaacc gtgagcggta
    15301 atcaccattg atttttcgtg gtcgtagccg cacacccagg ccgcgccgcg ggaacgcgat
    15361 ggcaaacgat cgcctttgca aatcgccata atgatgaatg gcagcagcgg gcaggcaatc
    15421 agcagcaacg tgatcattgg ttgagaaacg gtggtgttag caggctccag aggcagaggt
    15481 actgcagcag agagcatcgg cagcagccac ggcgcagcaa caccgccaat tacgcagcaa
    15541 atcgccagtg ccactacgct tacgctcatc aggagcggtg cacaggtggc gttttcggct
    15601 tctttggtac gcggcgcgcc gaggaacgtg acgccataga ctttcgccat acacatcacc
    15661 gccagcgcac cggtaattgc cagccccaca gcgagcagcg gccccagcag acgggcaaca
    15721 aacgcgccac tattgctcag tttgaaaaag gattgataga taacccattc cccggcaaac
    15781 ccgttcagcg gcggcagcgc agccattgcc atcaacccga ctaacatggc gatggagata
    15841 accggcattt tcttgccaat accaccgagt ttttcgatat cgcgatgacc ggtacggaac
    15901 cagacgctcc ccgccccgag gaacagtacg cttttgaaca ggctatggtt aagcagatgg
    15961 tacagaccac cgaccagacc aagggcaatc agcgccggtt gttcgagcgc gatacccgtt
    16021 acgccagcgc ccagccccag caggatgatg ccgatatttt ccagggtgtg gtaagccagc
    16081 aggcgctgga tgttgtgctc catcagcgca tacagaccac cgacaaacgc ggtgatcatg
    16141 ccgagcacca gcagcgcgat cccccaccac agcggtgcat taccgcccag cagtgacagg
    16201 gttaaaatgc ccagcaggcc aattttcatg actaccgtag aaaacaacgc ggcagctggc
    16261 gcagaggcgt tagcatgtgc ctgcggcacc cagccgtgca gcggaataat cccggccagc
    16321 aggccaaagc caatcacacc gagcagccag atatcggaac cgagcggcag ctgttgcata
    16381 cgcatatcca gcaggcgcag atccagcgtg ccataacgct gccacagcag ccagcaagca
    16441 atcaccagca gcagagtgcc aagacgcccc agcgcaaacc acagtttgcc ctctttgctg
    16501 ttgctggtga ggaacaccgc acacaaggcc atgacttcgg ccattaccac gaacatgccg
    16561 aggttgctgg caatgacggc gcagacggcg gcagccatca acatattgat ctgcaagccg
    16621 ttgcacttca cctgcgcgtg gcgatgccag tcaatgttgt agaggctgac aaacagaccg
    16681 cacagaccga gcgtaatcag ccaaatcgcg ttaagcggag agatttgcac atcgtagctt
    16741 accagcgaca gcgcaccgct cacgccaacc gcgccagtca gtacagtgaa gcccgcggct
    16801 gccgtataca gactaccaac cgcgccgcca attccggcta tccagccgct tagcgccttt
    16861 tgaaaagaaa agagaaatgc cagaacagcg gcggcgacaa accacgccac gccgctattg
    16921 atcagggaaa ttgcgctcat ttagcctctc cactttgagc ctgctgaaac aaggtgagat
    16981 cgccaaagtc cgtgttaaag gtcagctcac gcttacgttt gctgacgcgg gcgatatcgg
    17041 tgttatccac cagatgcagg gctttagtcg ggcacatccg cacgcaggcc ggaccttgtt
    17101 catcaaagct acaaaggtca catttgacgg cgatcgcgcg aatacctggc acccagtcaa
    17161 gcaatgtgct gacacgcgcc ggagcaggcg gtgccggtgg cgctttcggg gtattggcgt
    17221 ttgccggaat atccagcgga cggctgccgg aaaattcaat tgcgccaaac gggcaggcga
    17281 tgccgcacag tttgcagctt acgcacaggc tttcattcaa ctgcacggcc ccatcaacgc
    17341 gggtgatggc gttaaccggg cagaccaccg cgcagggtgc atcttcacag tggtgacaga
    17401 gctgcggcgc agattctttt tcattcagca tcactctcag gcgcggcatt gattgcaggc
    17461 cgtgctggcg atgcgtctct gaacaggcgg cctcacaagt gtggcagccg atacagagcg
    17521 tggagtcagc aattacaaaa cgattcacca ggcattcctc aggtgattgt catttttgac
    17581 gaaaacatgc cgatgaaatg tcattttcga cactcatcga cacgcccatc cccaaacagg
    17641 cgtaacgcct gcaaaacggg caaagcctca gctcatgctg ccgggctttg tccctttacc
    17701 agttggctta aattcaccgg cacattgttt tcaatggcgg tgtataagct gtcgtaaacg
    17761 ccagcgattt tttcgagata gtgttccaga acctgttcgc gatcgcggat gttgaccttg
    17821 ccatcaatca tgccgtcaat gctcagttgc gcctgcgcaa tccgctgttt gtcttcgcca
    17881 tcttttgttt cgacgtaata ctcgatggtg tggctgttaa agccatccgc cagggtgacg
    17941 taaatacgaa aatgttcaaa aaggacgaaa cagagttctt tgtccggcgc gcagctcatg
    18001 gcgtgatgca ggcgcgcttt cgataacgtg atcccctgaa ccagcgaatt gcagtagatg
    18061 tgccactggt cctgtaggcg acgatgccgc tgtgcgatgt aatcggcttt ctcgcttatt
    18121 tcccaaatag tcattgtcag gttacccgtt taacagagat gccagcttta agcatttttt
    18181 gtgccaactt ttaatttatt gttattaaag cgatttttaa gattttggtg aatttcgtcg
    18241 ccgtgtcgac gtgtcatttc gacatcatcg acattattca ctgcagggat aatcaacact
    18301 ggcacaatta ttgcttgtag ctggcaatag ttaatgggag gcgatatgca cgaaataacc
    18361 ctctgccaac gggcactgga attgatcgaa cagcaggccg caaaacacgg cgcaaaacgc
    18421 gtaactgggg tctggctcaa aattggcgca ttttcttgtg tcgaaaccag ctctcttgcc
    18481 ttttgttttg atctggtttg ccgcggcagc gtggcggaag gttgtaaact gcacctcgaa
    18541 gaacaagagg ccgaatgctg gtgtgaaaca tgccaacagt atgtgacgct actgacccag
    18601 cgcgtccgcc gctgtccaca gtgtcatggt gacatgctac agattgtggc agacgacggt
    18661 ttacagattc ggcggataga aatagaccag gagtgagcga tgtgtacaac atgcggttgc
    18721 ggtgaaggca acctgtatat cgagggtgat gaacataacc ctcattccgc gtttcgtagc
    18781 gcgccatttg ccccggcggc acgcccgaag atgaaaatca ccggcattaa aacgcctgaa
    18841 tttaccccca gccagactga agaaggcgac ctgcattacg gtcatggcga agcgggcact
    18901 cacgcgccag gcatgagcca gcgccggatg ctggaagtcg aaattgacgt gctggacaaa
    18961 aacaaccgtc tggctgaacg caaccgcgcg cgctttgctg cccgcaagca actggtgctc
    19021 aacctggttt ccagccctgg ttccggtaaa accaccctgc tgacggaaac cttaatgcgc
    19081 ctgaaagaca gcgttccgtg cgcggttatt gaaggtgacc agcaaaccgt gaacgatgcc
    19141 gcacgcattc gcgctaccgg cacaccagcg attcaggtga acaccggtaa aggctgccat
    19201 cttgacgcac agatgattgc cgacgccgca ccgcgtctgc cactggacga taacggtatt
    19261 ctgtttatcg aaaacgttgg caacctcgta tgcccggcca gcttcgatct cggtgaaaaa
    19321 cacaaagtgg cggtgctttc cgttaccgaa ggtgaagaca aaccgctgaa atatccgcat
    19381 atgtttgccg ccgcctcgct gatgctgctc aacaaagttg acctgttgcc gtatctcaac
    19441 tttgacgttg agaagtgcat cgcctgcgcc cgcgaagtca atccagaaat tgaaatcatc
    19501 cttatttccg ccaccagcgg cgaagggatg gaccagtggc tgaactggct ggagacacag
    19561 cgatgtgcat aggcgttccc ggccagatcc gcaccattga cggcaaccag gcgaaagtcg
    19621 acgtctgcgg cattcagcgc gatgtcgatt taacgttagt cggcagctgc gatgaaaacg
    19681 gtcagccgcg cgtgggccag tgggtactgg ttcacgttgg ctttgccatg agcgtaatta
    19741 atgaagccga agcacgcgac actctcgacg ccttacaaaa catgtttgac gttgagccgg
    19801 atgtcggcgc gctgttgtat ggcgaggaaa aataatgcgt tttgttgatg aatatcgcgc
    19861 gccggaacag gtgatgcagt taattgagca tctgcgcgaa cgtgcttcac atctctctta
    19921 caccgccgaa cgccctctgc ggattatgga agtgtgcggt ggtcataccc acgccatttt
    19981 taaattcggc ctcgaccagt tactgcctga aaacgttgag tttatccacg gtccgggttg
    20041 cccggtgtgc gtactgccga tgggcagaat cgacacctgc gtggagattg ccagccatcc
    20101 ggaagtcatc ttctgtacct ttggcgacgc catgcgcgtg ccggggaaac agggatcgct
    20161 gttgcaggca aaagcacgcg gtgccgatgt gcgcatcgtt tactcgccga tggatgcgtt
    20221 gaaactggcg caggagaatc caacccgcaa agtggtgttc ttcggcttag gttttgaaac
    20281 caccatgccg accaccgcta tcactctgca acaggcgaaa gcgcgtgatg tgcagaattt
    20341 ttacttcttc tgccagcaca ttacgcttat cccgacgctt cgcagtttgc tggaacagcc
    20401 ggataacggt atcgacgcgt tcctcgcgcc gggccacgtt agtatggtta tcggcactga
    20461 tgcctataat tttatcgcca gcgattttca gcgtccgctg gtggtcgctg gtttcgaacc
    20521 ccttgatcta ctgcaaggcg tggtgatgct ggtggagcag aaaatagcgg cccacagcaa
    20581 ggtagagaat cagtatcgtc gggtggtacc ggatgccggt aacctgctgg cgcaacaggc
    20641 gattgccgat gtgttctgtg tcaacggcga cagcgaatgg cgcggcttag gcgtgattga
    20701 atcttctggc gtgcacctga cgccggatta tcaacgattc gatgccgaag cacatttccg
    20761 cccggcaccg cagcaggtct gcgatgaccc gcgcgcgcgt tgtggcgaag tcttgacggg
    20821 caaatgtaag ccgcatcaat gcccgctgtt tggtaacacc tgtaatcctc aaaccgcgtt
    20881 tggtgcgctg atggtttcct ccgaaggagc gtgcgccgcg tggtatcagt atcgtcagca
    20941 ggagagtgaa gcgtgaataa tatccaactc gcccacggca gcggcggcca ggcgatgcag
    21001 caattaatca acagcctgtt tatggaagcc tttgccaacc cgtggctggc agagcaggaa
    21061 gatcaggcac gtcttgatct ggcgcaactg atagcggaag gcgaccgtct ggcgttctcc
    21121 accgacagtt acgttattga cccgctgttc ttccctggcg gtaatatcgg caagctggcg
    21181 atttgcggca cagccaatga cgttgcggtc agtggtgcca ttccgcgcta tctctcctgt
    21241 ggctttatcc tcgaagaagg attgccgatg gagacactga aagccgtagt gaccagcatg
    21301 gcagaaaccg cccgcacggc aggcattgcc atcgttactg gcgatactaa agtggtgcag
    21361 cgcggcgcgg cagataaact gtttatcaac accgcgggca tgggcgcaat tccggcgaat
    21421 attcactggg gcgcacagac gctaaccgca ggcgatgtat tgctggtgag cggtacactc
    21481 ggcgaccacg gggcgactat ccttaacctg cgtgagcagc tggggctgga tggcgaactg
    21541 gtcagcgact gcgcggtgct gacgccgctt attcagacgc tgcgtgacat tcccggcgtg
    21601 aaagcgctgc gtgatgccac ccgtggtggt gtaaacgcgg tggttcatga gttcgcggca
    21661 gcctgcggtt gcggtattga aatttctgaa tcagcgctgc cggttaaacc tgccgtgcgc
    21721 ggcgtttgcg aattgctggg actggacgcc ctgaactttg ccaacgaagg caaactggtg
    21781 atcgccgttg aacgcaacgc ggcagagcaa gtgctggcag cgttacattc ccatccactg
    21841 gggaaagacg cggcgctgat tggtgaagtg gtggaacgta aaggtgttcg tcttgccagt
    21901 ctgtatggcg tgaaacgaac cctcgattta ccacacgccg aaccgcttcc gcgtatatgc
    21961 taataaaatt ctaaatctcc tatagttagt caatgacctt ttgcaccgct ttgcggtgct
    22021 ttcctggaag aacaaaatgt catatacacc gatgagtgat ctcggacagc aagggttgtt
    22081 cgacatcact cggacactat tgcagcagcc cgatctggcc tcgctgtgtg aggctctttc
    22141 gcaactggta aagcgttctg cgctcgccga caacgcggct attgtgttgt ggcaagcgca
    22201 gactcaacgt gcgtcttatt acgcatcgcg tgaaaaagac acccccatta aatatgaaga
    22261 cgaaactgtt ctggcacacg gtccggtacg cagcattttg tcgcgccctg atacgctgca
    22321 ttgcagttac gaagaatttt gtgaaacctg gccgcagctg gccgcaggtg ggctataccc
    22381 aaaatttggt cactattgcc tgatgccact ggcggcggaa gggcatattt ttggtggctg
    22441 tgaatttatt cgttatgacg atcgcccctg gagcgaaaaa gagttcaatc gtctgcaaac
    22501 atttacgcag atcgtttctg tcgtcaccga acaaatccag agtcgcgtcg ttaacaatgt
    22561 cgactatgag ttgttatgcc gggaacgaga taacttccgc atcctggtcg ccatcaccaa
    22621 cgcggtgctt tcccgcctgg atatggacga actggtcagc gaagtcgcca aagaaatcca
    22681 ttactatttc gatattgacg atatcagtat cgtcttacgc agccaccgta aaaacaaact
    22741 caacatctac tccactcact atcttgataa acagcatccc gcccacgaac agagcgaagt
    22801 cgatgaagcc ggaaccctca ccgaacgcgt gttcaaaagt aaagagatgc tgctgattaa
    22861 tctccacgag cgggatgatt tagcccccta tgaacgcatg ttgttcgaca cctggggcaa
    22921 ccagattcaa accttgtgcc tgttaccgct gatgtctggc gacaccatgc tgggcgtgct
    22981 gaaactggcg caatgtgaag agaaagtgtt taccactacc aatctgaatt tactgcgcca
    23041 gattgccgaa cgtgtggcaa tcgctgtcga taacgccctc gcctatcagg aaatccatcg
    23101 tctgaaagaa cggctggttg atgaaaacct cgccctgacc gagcagctca acaatgttga
    23161 tagtgaattt ggcgagatta ttggccgcag cgaagccatg tacagcgtgc ttaaacaagt
    23221 tgaaatggtg gcgcaaagtg acagtaccgt gctgatcctc ggtgaaactg gcacgggtaa
    23281 agagctgatt gcccgtgcta tccataatct cagtgggcgt aataatcgcc gcatggtcaa
    23341 aatgaactgc gcggcgatgc ctgccggatt gctggagagc gatctgtttg gtcatgagcg
    23401 tggggctttt actggtgcca gcgcccagcg cattggtcgt tttgaactgg cggataaaag
    23461 ctccctgttc ctcgacgaag tgggcgatat gccactggag ttacagccga agttgctgcg
    23521 tgtattgcag gaacaggagt ttgaacgcct cggcagcaac aaaatcattc agacggacgt
    23581 gcgtctaatc gccgcgacta accgcgatct gaaaaaaatg gtcgccgacc gtgagttccg
    23641 tagcgatctc tattaccgcc tgaacgtatt cccgattcac cttccgccac tacgcgagcg
    23701 tccggaagat attccgctgc tggcgaaagc ctttaccttc aaaattgccc gtcgtctggg
    23761 gcgcaatatc gacagcattc ctgccgagac gttgcgcacc ttgagtaata tggagtggcc
    23821 gggtaacgta cgcgaactgg aaaacgtcat tgagcgcgcg gtattgctaa cacgcggcaa
    23881 cgtgctgcag ctgtcattgc cagatattgc tttaccggag cctgaaacgc cgcctgccgc
    23941 aacggttgtc gctcaggagg gcgaagatga atatcagttg attgttcgcg tgctgaaaga
    24001 aactaacggc gtcgttgccg ggcctaaagg cgctgcgcaa cgtctggggc tgaaacgcac
    24061 gaccctgctg tcacggatga agcgactggg aattgataaa tcggcattga tttaacagat
    24121 gtggttatac tcgatcaaca ttcaatgaaa ggaggcagta tggcagtttc agcaagaaat
    24181 caattaaccg gaacagttag cgcagtagca atgggcgcgg tgaatgacga agtagaactg
    24241 acgctggcag gtggtgcaaa gctggtggca attgtgactc acagcagcca gcaggctctg
    24301 ggtctggcga aagggaaaga agcgattgcc ctgattaaag ccccgtgggt aacgctggca
    24361 acggaagact gtggactgaa attctctgct cgcaaccagt ttgctggcag cgtgtcgaca
    24421 atcaccgaag gcgcagtgaa cgccactgtc catatcaaaa cggatgcggg ttttgaaatt
    24481 gtggcagtgg taacaaatga aagtcaggat gaaatgaaac tcaccactgg ctcacgcgtt
    24541 attgcgttga tcaaagcatc agcaatcctg attgctacca aagcgtaagc gccttatttg
    24601 ccggagagta tggctctccg gcagatttat ttctttggac gatatttttc tggcaactcc
    24661 ggcaccggac gcttgtcatc gatgagatga cgcacggtta agattggatg acgccacagc
    24721 attctcggcc cggcccaacg cataatctgt ttcatctctt cacgctttgc aggctggtaa
    24781 cagtgcaccg gacactgctt acaggctggt ttctcttcgc cgaacacaca tttatccagc
    24841 cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg gctccgctga cgcctgcggg
    24901 catttcgctt gataaagatc gatcattttt ttaatcgtca gtttttcacg agagatacgc
    24961 ttgccggaca tactgcctcc atactcttaa ggtgtattta tactacaact taatttgaaa
    25021 ggggactatg gctctgaagg aggaggttta ttcaaccgat acaattttgc gtacttgctt
    25081 cataagcatc actcaaaagc tgcaaaacag catctttccc ggaaccagca tcaagaactc
    25141 gccgttcgct tcttcccctg aaatgattaa ctccggtatc atgtgcgcct tatgtgatta
    25201 caacgaaaat aaaaaccatc acaccccatt taatatcagg gaaccggaca taaccccatg
    25261 agtgcaatag aaaatttcga cgcccatacg cccatgatgc agcagtatct caagctgaaa
    25321 gcccagcatc ccgagatcct gctgttttac cggatgggtg atttttatga actgttttat
    25381 gacgacgcaa aacgcgcgtc gcaactgctg gatatttcac tgaccaaacg aggtgcttca
    25441 gcgggagagc cgatcccgat ggcggggatc ccctaccatg cggtggaaaa ctacctcgcc
    25501 aaactggtga atcagggcga gtccgttgcc atctgcgaac aaattggcga tccggcgacc
    25561 agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta cgccaggcac catcagcgat
    25621 gaagccctgt tgcaggagcg tcaggacaac ctgttggcgg ctatctggca ggacagcaaa
    25681 ggtttcggct acgcgacgct ggatatcagt tccgggcgtt ttcgcctgag cgaaccggct
    25741 gaccgggaaa cgatggcggc agaactgcaa cgcactaatc ctgcggaact gctgtatgca
    25801 gaagattttg ctgaaatgtc gttaattgaa ggccgtcgcg gcctgcgccg tcgcccgctg
    25861 tgggagtttg aaatcgacac cgcgcgccag cagttgaatc tgcaatttgg cactcgcgat
    25921 ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt gtgctgccgg ttgtctgttg
    25981 cagtatgcga aagataccca acgtacgact ctgccgcata ttcgttccat caccatggaa
    26041 cgtgagcagg acagcatcat tatggatgcc gcgacgcgtc gtaatctgga aatcacccag
    26101 aacctggcgg gtggtgcgga aaatacgctg gcttctgtgc tcgactgcac cgtcacgccg
    26161 atgggcagcc gtatgctgaa acgctggctg catatgccag tgcgcgatac ccgcgtgttg
    26221 cttgagcgcc agcaaactat tggcgcattg caggatttca ccgccgagtt acagccggta
    26281 ctgcgtcagg tgggcgatct ggaacgtatt ctggcgcgtc tggcgttgcg tactgctcgc
    26341 ccgcgcgatc tggcccgtat gcgccatgct ttccagcaac tgccggaact gcgcgcgcag
    26401 ttagaaaatg tcgatagtgc accggtacag gcgctgcgtg agaagatggg cgagtttgcc
    26461 gaactgcgcg atctgctgga gcgggcaatc atcgacacac caccagtgct ggtacgcgac
    26521 ggtggtgtta tcgcaccggg ctataacgaa gagctggatg agtggcgggc gctggctgac
    26581 ggcgcgaccg attatctgga gcgtctggaa atccgcgagc gtgaacgtac cggcctggac
    26641 acgctgaaag tcggatttaa cgcggtgcat ggttattaca ttcagatcag ccgtggacaa
    26701 agccatctgg cccccatcaa ctacatgcgt cgccagacgc tgaaaaatgc cgagcgctac
    26761 attattccag agctaaaaga gtacgaagat aaagttctca cctcaaaagg caaagcactg
    26821 gcactggaaa aacagcttta tgaagagctg ttcgacctgc tgttgccgca tctggaagcg
    26881 ttgcaacaga gcgcaagcgc gctggcagaa cttgatgtgc tggttaacct ggcggagcgg
    26941 gcctataccc tgaactacac ctgcccgact tttattgata aaccgggtat tcgcatcacc
    27001 gaaggccgcc atccggtggt tgaacaggtg ctgaatgagc cgtttatcgc caacccgctg
    27061 aacctgtcgc cacagcgtcg catgttgatc attaccggtc cgaatatggg cggtaaaagt
    27121 acctatatgc gccagaccgc actgattgcg ctgatggcct acatcggcag ctatgtaccg
    27181 gcacaaaaag ttgagattgg acctatcgat cgcatcttta cccgcgtagg ggcagcggat
    27241 gatctggcat ccgggcgttc aacctttatg gtggaaatga ccgaaaccgc caatatttta
    27301 cataacgcca ccgaatacag tctggtgtta atggatgaga tcgggcgtgg aacgtccact
    27361 tacgatggtc tgtcactggc atgggcgtgc gcggaaaatc tggcgaataa gattaaggca
    27421 ttgacgctgt ttgctaccca ctatttcgag ctgacccagt taccggagaa aatggaaggc
    27481 gtcgccaacg tgcatctcga tgcactggag cacggcgaca ccattgcctt tatgcatagc
    27541 gtgcaggatg gcgcggcgag caaaagctac ggcctggcgg ttgcagctct ggcgggcgtg
    27601 ccaaaagagg tgattaagcg cgcacggcaa aaactgcgtg agctggaaag tatttcgccg
    27661 aacgccgccg ctacacaagt ggatggtacg caaatgtctt tgctgtcagt accggaagaa
    27721 acctcgccag cggtcgaagc tctggaaaat ctcgacccgg attcactgac tccgcgtcag
    27781 gcgctggaat ggatttatcg cttgaagagt ctggtgtaat aacaattccc gatagaaaaa
    27841 tactatcggg aatattatct acagctgacg aatcaaataa aaataccctg tataatagga
    27901 aagtttattt tacagggtaa aaccatgcca tctacacgct atcaaaaaat caatgcccat
    27961 cactatcgcc atatatgggt cgttggtgat attcatggtg aatatcagtt attacaatcc
    28021 cgcttacatc aactctcttt tttccccgaa accgacttac ttatttctgt cggcgataat
    28081 attgatcgtg ggccggagag tcttgacgtc ctgcgcctgc taaaccagcc ctggtttatc
    28141 tctgttaaag gcaaccacga agcaatggcg ctggatgcgt tcgcgaccgg cgatggcaat
    28201 atgtggcttg ccagcggtgg tgactggttc tttgagttaa atgatatgga acaaaaagaa
    28261 gctatagg
//
LOCUS       DAZKFU020000037        28157 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZKFU020000037 DAZKFU020000000
VERSION     DAZKFU020000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.037"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(27..1424)
                     /gene="ccp"
                     /locus_tag="ACHOQQ_RS06390"
                     /old_locus_tag="pgaptmp_001278"
     CDS             complement(27..1424)
                     /gene="ccp"
                     /locus_tag="ACHOQQ_RS06390"
                     /old_locus_tag="pgaptmp_001278"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGU4469553.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1829..3478
                     /locus_tag="ACHOQQ_RS06395"
                     /old_locus_tag="pgaptmp_001279"
     CDS             1829..3478
                     /locus_tag="ACHOQQ_RS06395"
                     /old_locus_tag="pgaptmp_001279"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4469554.1"
                     /translation="MLNQKIQNPNPDELMIEIDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3529..4131)
                     /locus_tag="ACHOQQ_RS06400"
                     /old_locus_tag="pgaptmp_001280"
     CDS             complement(3529..4131)
                     /locus_tag="ACHOQQ_RS06400"
                     /old_locus_tag="pgaptmp_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU4469555.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4651..5550
                     /locus_tag="ACHOQQ_RS06405"
                     /old_locus_tag="pgaptmp_001281"
     CDS             4651..5550
                     /locus_tag="ACHOQQ_RS06405"
                     /old_locus_tag="pgaptmp_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4469556.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5599..6612
                     /gene="yhjD"
                     /locus_tag="ACHOQQ_RS06410"
                     /old_locus_tag="pgaptmp_001282"
     CDS             5599..6612
                     /gene="yhjD"
                     /locus_tag="ACHOQQ_RS06410"
                     /old_locus_tag="pgaptmp_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGU4469557.1"
                     /translation="MTQENEIKRPTQDLEHEPIKPLDNSEKGGKVSQALETVTTTAEK
                     VQRQPIIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7005..8327
                     /locus_tag="ACHOQQ_RS06415"
                     /old_locus_tag="pgaptmp_001283"
     CDS             7005..8327
                     /locus_tag="ACHOQQ_RS06415"
                     /old_locus_tag="pgaptmp_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4469558.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8509..10569)
                     /locus_tag="ACHOQQ_RS06420"
                     /old_locus_tag="pgaptmp_001284"
     CDS             complement(8509..10569)
                     /locus_tag="ACHOQQ_RS06420"
                     /old_locus_tag="pgaptmp_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4469559.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10639..11406)
                     /gene="pdeH"
                     /locus_tag="ACHOQQ_RS06425"
                     /old_locus_tag="pgaptmp_001285"
     CDS             complement(10639..11406)
                     /gene="pdeH"
                     /locus_tag="ACHOQQ_RS06425"
                     /old_locus_tag="pgaptmp_001285"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGU4469560.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11638..12567
                     /gene="kdgK"
                     /locus_tag="ACHOQQ_RS06430"
                     /old_locus_tag="pgaptmp_001286"
     CDS             11638..12567
                     /gene="kdgK"
                     /locus_tag="ACHOQQ_RS06430"
                     /old_locus_tag="pgaptmp_001286"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGU4469561.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICAELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12663..14159)
                     /locus_tag="ACHOQQ_RS06435"
                     /old_locus_tag="pgaptmp_001287"
     CDS             complement(12663..14159)
                     /locus_tag="ACHOQQ_RS06435"
                     /old_locus_tag="pgaptmp_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4469562.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14380..15666)
                     /gene="dctA"
                     /locus_tag="ACHOQQ_RS06440"
                     /old_locus_tag="pgaptmp_001288"
     CDS             complement(14380..15666)
                     /gene="dctA"
                     /locus_tag="ACHOQQ_RS06440"
                     /old_locus_tag="pgaptmp_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGU4469563.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15849..17837)
                     /gene="hmsP"
                     /locus_tag="ACHOQQ_RS06445"
                     /old_locus_tag="pgaptmp_001289"
     CDS             complement(15849..17837)
                     /gene="hmsP"
                     /locus_tag="ACHOQQ_RS06445"
                     /old_locus_tag="pgaptmp_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGU4469564.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17919..21392)
                     /gene="bcsC"
                     /locus_tag="ACHOQQ_RS06450"
                     /old_locus_tag="pgaptmp_001290"
     CDS             complement(17919..21392)
                     /gene="bcsC"
                     /locus_tag="ACHOQQ_RS06450"
                     /old_locus_tag="pgaptmp_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGU4469565.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLHQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21374..22480)
                     /gene="bcsZ"
                     /locus_tag="ACHOQQ_RS06455"
                     /old_locus_tag="pgaptmp_001291"
     CDS             complement(21374..22480)
                     /gene="bcsZ"
                     /locus_tag="ACHOQQ_RS06455"
                     /old_locus_tag="pgaptmp_001291"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGU4469566.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22487..24826)
                     /gene="bcsB"
                     /locus_tag="ACHOQQ_RS06460"
                     /old_locus_tag="pgaptmp_001292"
     CDS             complement(22487..24826)
                     /gene="bcsB"
                     /locus_tag="ACHOQQ_RS06460"
                     /old_locus_tag="pgaptmp_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGU4469567.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAIAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24837..27455)
                     /gene="bcsA"
                     /locus_tag="ACHOQQ_RS06465"
                     /old_locus_tag="pgaptmp_001293"
     CDS             complement(24837..27455)
                     /gene="bcsA"
                     /locus_tag="ACHOQQ_RS06465"
                     /old_locus_tag="pgaptmp_001293"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HGU4469568.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27452..>28157)
                     /gene="bcsQ"
                     /locus_tag="ACHOQQ_RS06470"
                     /old_locus_tag="pgaptmp_001294"
     CDS             complement(27452..>28157)
                     /gene="bcsQ"
                     /locus_tag="ACHOQQ_RS06470"
                     /old_locus_tag="pgaptmp_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HGU4469569.1"
                     /translation="TITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWAR
                     AMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKSSGRY
                     QWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIG
                     SQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANW
                     CLLNYSGLKTPVGSKS"
BASE COUNT         6613 a         7789 c         7111 g         6644 t
ORIGIN      
        1 cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg cgtgtaaacc
       61 ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg cagctctttg
      121 cctacctgat agcgcagcat cagtttcacc gccccgtcca gcgtcggcac gtcaccgcga
      181 tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg acgcaattta
      241 tcacgctctt ctttagtcac attcatacga ccaatatccg ccgccgtaat ttccccaaag
      301 ttaaagtctt ttttcagccc caacggttcg aaggaacgtc cgccgagaat aattccacca
      361 tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg ttgcgccgtc
      421 agcgcattct catctccacg caaccattta tcaaatgggg aatccggcgt aattaatgtt
      481 ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg cggatagact
      541 tcgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat ttcgtcccag
      601 gatttcgacg ccatttcaat cgggttcaac ggcggtccac cagcctgatc ctgcaatgtt
      661 gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac cgtcggcgcg
      721 ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc gacgcccccc
      781 gcattcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa acggggatcg
      841 tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt ttgcgggatg
      901 ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta atattccgcg
      961 cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac cttacccgcc
     1021 cagtgtagcg cggtataacg cgttggtggc atagtttcat actgcatcac ccattcaatc
     1081 ttattcagat cgctttgcga aacgggttta tcagccagca gtgccgcacg cacggcctcg
     1141 aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt cgcgccagga
     1201 atataataat aggcgggtaa ttctgccgaa ggcgtgtggc aatagtcgca tcctttttcg
     1261 cggagaaagc ctaaaacctt attattttca ctgacagcag atgcctgaac atcggcctgt
     1321 ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata gcaaatcgcg
     1381 acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct tccctcacgg
     1441 tggtgagtta ttgtgaccca gaaaattttc cagaatgctg caaacgtctc tgcgagacaa
     1501 agccatcata aaaaacaggg agatacactt tttgatagga atcaatcaat tacatgaata
     1561 taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca tatcatgatt
     1621 gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acaccaaatc tgtattttta
     1681 taaattccat ggatattagt gcgttatatt ttttgacagg ttaataaacg tgatccaccg
     1741 cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag cccgataatt
     1801 agcgacgaat ttcggaggtt ggatccttat gctcaatcag aaaattcaaa accctaatcc
     1861 agacgaactg atgatcgaaa tcgatctctg ctatgaactg gacccgtatg aattaaaact
     1921 ggatgagatg atcgaggcag aaccggaacc cgagatgatt gaagggctgc ccgcctctga
     1981 tgcgctgacg cctgccgatc gctatctcga actgttcgag catgttcagt cggcgaaaat
     2041 tttccccgac agtaaaacct ttcccgactg cgcacctaaa atggacccgc tggatatttt
     2101 aatccgctac cgtaaagtgc gccgtcatcg tgattttgac ttgcgcaagt ttgttgaaaa
     2161 ccacttctgg ctgccggagg tctactccag cgagtatgta tcggacccgc aaaattccct
     2221 gaaagagcat atcgaccagc tgtggccggt gctaacccgc gaaccacagg atcacattcc
     2281 gtggtcttct ctgctggcgc tgccgcagtc atatattgtc ccgggcggcc gttttagcga
     2341 aacctactat tgggattcct atttcaccat gctggggctg gcggaaagtg gtcgggaaga
     2401 tttgctgaaa tgcatggccg ataacttcgc ctggatgatc gaaaactacg gtcacatccc
     2461 caacggcaac cgcacctatt atttgagccg ctcgcaacca ccggtttttg cgctgatggt
     2521 ggagttgttt gaagaagatg gtgtacgcgg tgcacgccgc tatctcgacc acctgaaaat
     2581 ggaatatgcc ttctggatgg acggtgcaga atcgttgatc cctaatcagg cctatcgcca
     2641 tgttgtgcgg atgccggacg gatcgctgct caaccgttac tgggacgatc gcgacacgcc
     2701 gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa cattctggtc gcccgcccaa
     2761 cgaggtgtac cgcgatttac gcgcgggggc ggcctccggt tgggattact cttcccgttg
     2821 gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc cagttcatcc ccatcgatct
     2881 gaatgccttc ctgtttaaac tggagagcgc catcgccaac atctcggcgc tgaaaggtga
     2941 gaaagagaca gaagcactgt tccgccagaa ggccagtgcc cgtcgcgatg cggtaaaccg
     3001 ttacctctgg gatgatgaaa acggcatcta ccgcgattac gactggcgac gcgaacaact
     3061 ggcgctgttt tccgctgccg ccattgtgcc actctatgtc ggtatggcga accatgaaca
     3121 ggccgatcgt ctggcaaacg ccgtgcgcag tcggttactg acacctggcg ggattctggc
     3181 aagcgagtac gaaaccggtg aacagtggga taaacccaac ggctgggcac cgttacaatg
     3241 gatggcgatt cagggattta aaatgtatgg agatgacctt ctgggtgatg aaatcgcgcg
     3301 cagctggctg aagacggtga atcagttcta tctggaacag cacaaactga tcgagaaata
     3361 ccatattgcc gatggtgttc cccgcgaagg cggcggtggc gagtatccgt tgcaggatgg
     3421 gtttggctgg actaacggtg tggtacgccg tttaattggt ttgtacggcg aaccataata
     3481 tttttacagc cagccgctaa cttcctgctg gctgtaaaat tattctcttc aggaggagat
     3541 atttaacatc attgccgcct gggtgcgatt tttcacttcc agacggcgat acagggattc
     3601 cagatgcgct tttaccgttc cggtactgat attcagcgcc ctgccgatct ccttatttga
     3661 ttcgcccgcc gctaacatgg ttaaaatctc ccgctggcgg gcgcttaacg atttgagatc
     3721 tttaatgtcc ttttccggcg tcgtccgcca gtctccaggc agaaacatca tccccatcgc
     3781 cgcactattt accgccaacg caaatgtctc gacggttgaa tcacgaggca caatggccag
     3841 cacattaaaa tgaataactt cctgtaacca ccgtttattg caatccgtcg ccgtaattaa
     3901 caccttaacc tcaggaaatt gcaccacggt tttttgcagc aaccagtagc aaaactcacc
     3961 atcctgatcg ccatcgagca taactaaggc ttcagggtaa ctttccagct tttgccatag
     4021 ctcgtctgcc tgactggccc cctgaatact cactcctgga atacgctgct gtaaactgat
     4081 tttcattcca tgaataaata ttgactgcct gtcaaacatg actatttgca taactgaatc
     4141 tccacctgaa tacgttaaaa agacttaagt agtggaaggg tattacccgc gagaaaaaat
     4201 aagaattcgc catttggcgg tggccattct acagagatgg cgcgtagaaa atagttaccg
     4261 atataaatag ttacagctaa acgcctgaaa ttacatgtcg agggcactat ttaaaacaat
     4321 tttgaggatt tccttatatt aatggttagt acgcatgcaa ttaaaaatga aattccgcga
     4381 ccacaagcca aaataacaaa tggcaatgag acaaaaataa gcacaaatag ccaatacgtc
     4441 ctctattcac tttaaaggga atcgctgaaa aatacgctct gtttaagggg attcaccttt
     4501 ctcagaaagc tattccgccc ttttcctgct gagaaatcgc cacattcgtc atgacaacat
     4561 tgtgaaaccc ggcattagat gttagaaaaa acaataaaca atgcgatatg cgcgttactg
     4621 tttcgctgtg aacaacaatc ggtcaaagaa atggataaaa ttcacgcaat gcagttgttc
     4681 atcaaagtcg cggagctgga aagtttttcc cgcgcggcgg atttctttgc tttgccaaaa
     4741 ggaagtgttt cgcgccagat acaggcactg gaacatcaac ttggcaccca gcttctccag
     4801 cgcaccacgc gacgggtcaa actcacgcca gaaggcatga cctattatca acgagcaaaa
     4861 gatgtgttga gtaatctcag cgaactggac ggtctgtttc aacaggatgc caccagtatc
     4921 agcggtaaat tacgcatcga catcccgcca ggaatcgcga aaagcctgtt actgccgcgc
     4981 ctgtcggaat ttctctatct gcatccggga attgagctgg aactgagtag ccatgaccgt
     5041 ccggtagata ttcttcatga tggttttgat tgcgtgatac gcactggcgc gttaccggaa
     5101 gatggcgtta tcgcccgtcc cctcggcaaa ctgaccatgg tcaactgtgc cagtccgcac
     5161 tatctgacgc gctttggtta tccgcaaagc cccgatgatc tgacttcaca cgcaatagtg
     5221 cgttatacac cgcacctggg tgtacatccg ttaggttttg aggttgccag cgttaatggc
     5281 gtccagtggt ttaagtctgg cggcatgttg acggtaaaca gtagcgaaaa ctatctcgcc
     5341 gccggtcttg ccggtctggg gattattcag atcccgcgca ttgccgtgcg cgaagccctg
     5401 cgtgccgggc ggcttattga agtgttgcca ggctaccgtg ccgagccgct ctccctttcg
     5461 ctggtttatc cgcagcgtcg ggagctttcc cggcgtgtaa acctgtttat gcagtggctg
     5521 gctggcgtaa tgaaagagta cctggactga ccgactatac tttttaagaa tgaccacgac
     5581 agaaggacaa aagagcggat gacgcaggaa aacgagatca aacgtcccac ccaggatctg
     5641 gagcacgagc cgattaagcc gttggataat agcgagaaag gcggcaaagt tagccaggcg
     5701 ctggaaaccg tcaccaccac tgccgaaaaa gtccaacgcc aaccgattat tgcgcacctt
     5761 attcgcgcga cagaacgctt taacgatcgg ctgggtaacc agtttggtgc ggctatcacc
     5821 tatttctcat ttttgtcgat gatcccgatt ttgatggtgt cgtttgccgc agggggcttt
     5881 gtgctggcct cccatccgat gttgctacag gatatcttcg acaaaattct gcaaaacatc
     5941 agcgatccga cgctagccgc tacgttgaaa aacaccatca acaccgccgt tcagcagcgt
     6001 acgactgtag ggcttgtcgg cctggcggtg gcgctttatt ccggcatcaa ctggatgggt
     6061 aacctgcgtg aagcgattcg cgcccagtcg cgcgatgtct gggaacgctc gccgcaagat
     6121 caggagaagt tctgggtaaa atatctgcgt gattttattt cgctgattgg tttgttgatt
     6181 gcgctgattg tgacgctttc gatcacctcg gttgccggtt cggcgcagca aatgattatt
     6241 agcgccctgc acctgaacag cattgagtgg ctgaaaccga cgtggcggtt gattggcctg
     6301 gcgatttcca tcttcgccaa ctatctgctt ttcttctgga tcttctggcg gttaccgcgc
     6361 caccgtccgc gcaaaaaagc cctgattcgc ggaacatttc tggctgctat tggttttgaa
     6421 gtgattaaaa tcgtgatgac ctacacccta ccatcgctga tgaaatcccc ctctggcgca
     6481 gcatttggtt ccgtgctggg gctgatggca tttttctact tcttcgcccg tttgacgctg
     6541 ttttgcgcgg cgtggattgc caccgccgaa tataaagacg acccgcgaat gccggggaaa
     6601 acacagcctt aaaataggcc ggatacggcg tacgaattta ttcggcctgg ctccccgtag
     6661 gccggataag atgcgccagc atcgcatccg gctataatgc gcacataacc tcttgaaact
     6721 cttccccaga gcctcttcag ccatctattt gggagcaaac aatttcatgc caactcataa
     6781 ccccagcata taaatccagt tggtaacttt tatttaacct gaaaccagtt ttatccacca
     6841 tttataaaat tatgtgaagc atttcataga agaaaaatca ctggcctaaa cattatcccc
     6901 tttttgcctt atttttgacc atttccgcga tttgttacac attgaaatat cacttttgct
     6961 gtgcgtaata tggctattcg ttagccaaaa aataagaaaa gattatgcaa gcaacagcca
     7021 caacactcga ccacgagcaa gaatacacgc cgatcaactc gcgtaataaa gtccttgtcg
     7081 cctctctcat tggcacggcc attgagttct tcgactttta catttacgcc actgcggccg
     7141 ttattgtgtt tccgcatatc ttcttcccgc agggcgatcc aacggcagca acgctacagt
     7201 cgctcgccac cttcgccatc gccttcgtcg cgcgccccat tggctctgcc gtatttggtc
     7261 attttggcga tcgcgttggg cgtaaagcga cgctggtcgc ctcgttgcta acgatgggga
     7321 tttcgaccgt ggtgattggt ctgctgccgg gctatgccac gattggtatt ttcgccccgc
     7381 tgctgctggc gctggctcga tttggtcagg gtcttggctt aggcggtgaa tggggcggtg
     7441 cggcgctgct ggcgactgaa aacgccccac cgcgcaaacg tgctctgtat ggctccttcc
     7501 cacagctggg cgcaccgatt ggcttcttct ttgccaacgg cactttcttg ctgctttcct
     7561 ggctactgac cgacgagcag tttatgagct ggggctggcg tgtgccattt atcttctcgg
     7621 cggtgctggt cattatcggc ctgtatgttc gcgtgtcgct gcatgagtcg ccggtgtttg
     7681 agaaagtcgc taaagcgaaa aaacaggtga agatcccgct gggtacgctg ctgaccaaac
     7741 atgttcgcgt aaccgtactg ggtacgttca ttatgctggc aacctatacg ctattttaca
     7801 tcatgacggt ctactctatg acctttagta ccgccgccgc gccagttggg cttggcctgc
     7861 cgcgcaacga agtgttgtgg atgctgatga tggcagttat tggttttggc gtgatggtgc
     7921 cagtcgccgg attactggct gatgcctttg gtcgccgtaa aagcatggta attatcacca
     7981 cgctgatcat cctgttcgcg ctgttcgcct ttaacccgct gctcggttct ggcaacccga
     8041 ttctggtttt tgccttcctg ctgctggggt taagtctgat ggggctgacc ttcgggccaa
     8101 tgggtgcgct gttaccagag ctgtttccga cagaagtgcg ttacaccgga gcatcgttct
     8161 cttacaacgt agcgtcgatt ctcggggctt ccgttgcgcc atatatcgca gcctggttgc
     8221 agactaacta cgggctaggt gcggtggggt tatatctggc ggcgatggct ggcttgacgt
     8281 taatcgccct gctgctgacc catgagacgc gacatcagtc gttgtaataa gagtgaaaaa
     8341 cctgatgcgc tatgcttatc aggcctgaat ttccatacaa tatattgaat tcacaaggat
     8401 ttgtaggccg gataaggcgt taacgccgca tccggcatga acaaagcaca ctttgccaac
     8461 aatctaaatc tgtttaattg ccggatgtca gacatccggc aattatcatc acttcttcat
     8521 ctgcgacaaa atcgtccgac actgattagc ctccccttcg gaaggtgaga tcagcgccag
     8581 taacgccgcc gccggtgtta ccagcgttgc cagtgccgca gcaacagcac cacgggcaat
     8641 caacggcccg gctttcaccc cagcctgagg atttttaaac gtcccacgca catacagcgg
     8701 cgaacgcagt gtgataatcc gaatcccttt actctccgga tcaatagtca aatccagctg
     8761 ttccgaagca aaacttgccg tgccggtaac gttaatcaac gcgttctcag tatcgaaagc
     8821 aaaaatctgc gggcgtgcca cgccgttggc aatattcaga ttcgccgccg cgcagttcac
     8881 ccgcacctca tcgtcaccaa atatcgcacc gacaatgtag ttgccgacat tcagcccaac
     8941 aatctccatc aggttgcggc tcaccagccc gtcattcatc aacagtttca ggttgccgtt
     9001 actgttgcct aaaagcgccg ccaccgagtt accgctaccg cgtagttccg cgtcaccgtt
     9061 catttccccc agcgtcttct gcatcagttc cacatcgggc atcagttctt tcagtttcag
     9121 tcgacgagcc tgaatatctg cccgcccctg catcggcttt ttatcgcctt ccagatgaat
     9181 attcgccgca atgctgccac ccgccatgcc aaatttcagc ggttgcaggc gcaggtcagc
     9241 atttttgagg atgatatgag tagaaagatc gctaatcggc aggctactgc catgctcgat
     9301 acgtcggcct ttgaaacgaa catcggcatc catgacgtcc catttatcgg tttcgaagcg
     9361 gtcataaggc agcactttac ccgcaggctg aacgcttttt tcgcccttct tctgttcaga
     9421 ccgtttcgac ttttcagccc ctttcccgga atcaacgcca atcaacggtc ccaggtccgc
     9481 cagccgcaat tgccgcgact cgacatcacc ttccagtttt ggtcgtggct ttccggtggt
     9541 gtagaccaga gaaccgtgga tatcgctatc gccaatgcgc ccattaaaac cgcgataatc
     9601 aaagaccgac gatttttcag tgtcgatttt cgctaccagc cgaccatccg tttcaaacgg
     9661 cggggtatcg ggcagcagaa cgcccgtcag ttcatagaga tcacccagtg aatcgccaga
     9721 aaatttaagc cgtaaatcga caccgcccat cttcattggg tcattcacga cgccatcaaa
     9781 agcaacacgg gtattacctg aacggaaatc agcctgtacc ggaaacggcg tcccttcgcc
     9841 acgcagcgcc agcataccgc ctatttttcc cgtaccagtg agcggttcac cgttataacg
     9901 tccctgcgcc ttcaggccaa aaacgtaatc gcccaccttt tctttatccg ctttaccttt
     9961 cgatccagta acttcgctga acggcagtgg cttgcctaac ggatcgacaa aaatttccag
    10021 atccgctttg cttactttgt catcaatggc gatccgccct tgatcgaaaa gaatattatc
    10081 cagccgaaac gaccatgccg acggctttgc attcgcgtct ttgttgtcat cgttggcaag
    10141 attaaacgtc cagttattgt tcttttcaga gaggcgaatc aggcgtgcgt cgggcttttc
    10201 cagtttgatc cacggtagcc agaccgtttt ggtcagcagc gccaacggtg ccagcgttgc
    10261 ctctacgcgt ggcaaatgca ccatcgtgac ttcgggaata tccggtggat tgccaagaat
    10321 gatgtcttcc gcatgtacat ggggccacgg cacccagctg cgccagccag tttcttgttt
    10381 ttgccgctcc cacaccacgc ccagatcgcc acggatagcg aacggacgat tcaactccgc
    10441 agagactttc tggttgatgg tcggtttgag tcgattccag tcaaaagttg caatcaaaat
    10501 gatcgccacg acaatcaaca acaagaaagc ccctgaaatc gcagcggtta ttttgcctgc
    10561 cttgctcatc gttcgcttcc tcatattctt cctgtgccag tcctaaagat agtccagcca
    10621 ggcggaaaat gaggcagctt atagcgccag aaccgccgta ttcagcgttt ctatcggtgc
    10681 cgggcgtgaa agaaaccagc cttgtgcggc gaatgcgggc gagttctgaa catcacgcca
    10741 ctcttccggt gtttctacgc cctcgacaat caccccgcga caatagcgat tcattagatg
    10801 taaaagctga gaaaagagtg tgcggccttc cggcgactga cgcagcatca caaacagttc
    10861 tcgcgcgatt ttgatgtagt cataacgcac ttcacttagt gcagagaaat ttgccatccc
    10921 ggtaccaaaa tcatccagcc acagcgggcc aaattcacac atcgaggcaa aggttgaatc
    10981 tttcggcaga cggatatgct ccaccagttc gaaacgcagc cagggaagac gctcaatttg
    11041 gcgcaggatt tttggttgct gacgcagggc gatgagcgta gggccatcaa tattgaccga
    11101 tgccagcagg ccgtgctcta taaagaagtc ggctttttgc gccagcaaat caatctgctc
    11161 tttcacaacc tccatccgat ggctgacggt gatttcagta aaatagcgat ccggcggcag
    11221 gcgttgcgaa gggttcaagg gatgcgtgac caccgttaat agctccacgg ccattaaccg
    11281 cccgcatgtt tgatagatcg gctgccaagt gtaagcacgc tcacactgca accaaaaacg
    11341 ccgttcctgc aagctctcga tgcttgcttc agggttgctt attcgctgga taacctgcct
    11401 tatcatcaaa gatgtcctgt gtttaaaagt gatgctgccc ggactcgaca aagattatcg
    11461 gcgcgtaagg gcagaacttt accgcctggc cggggtaaat ttcctcactc cacaacggaa
    11521 tctttcagga atacgttctg gctcaaaaaa ataatggaac actgttttaa tatggttgac
    11581 cagcaaacca ccacagcgca aactaacgct aattttttac agatcaggtt cacgactatg
    11641 tccaaaaaga ttgccgtgat tggcgaatgc atgattgagc tttccgagaa aggcgcggac
    11701 gttaagcgcg gtttcggcgg cgatacactg aatacttccg tctatatcgc ccgtcaggtc
    11761 gatcctgcgg cattaaccgt tcattacgta acggcgctgg gaacggacag ttttagccag
    11821 caaatgctgg acgcctggca cggcgagaac gttgatactt ccctgaccca acggatggaa
    11881 aaccgtctgc cgggccttta ctacattgaa accgacagca ccggtgaacg tacgttctac
    11941 tactggcgta acgaagccgc tgccaaattc tggctggaga gcgagcagtc tgcggcgatt
    12001 tgtgcagagc tggcgaattt cgattatctc tacctgagcg ggattagcct ggcgatctta
    12061 agcccgacca gccgcgaaaa gctgctttcc ctgctgcgcg aatgccgcgc caacggcgga
    12121 aaagtgattt tcgacaataa ctatcgtccg cgcctgtggg ccagcaaaga agagacacag
    12181 caggtgtacc aacaaatgct ggaatgcacg gatatcgcct tcctgacgct ggacgatgaa
    12241 gacgcgctgt ggggtcaaca gccggtggaa gacgtcattg cgcgcaccca taacgcgggc
    12301 gtgaaagaag tggtggtgaa acgcggggcg gattcttgcc tggtgtctat tgctggcgaa
    12361 gggttagtgg atgttccagc ggtgaaactg ccgaaagaaa aagtgatcga taccaccgca
    12421 gctggcgact ctttcagtgc cggttatctg gcggtacgtt tgacgggcgg cagtgcggaa
    12481 aacgcagcga aacgcgggca cctgaccgca agtaccgtta ttcagtatcg cggcgcgatt
    12541 atcccgcgtg aggcgatgcc agcgtaaacg cagcatatgc aaaaggctgg ataaagtgtt
    12601 acccctctat ccagctcatc cataaatacc tgatggttgt gctaactcat caccatcagg
    12661 cattactgtg caggtggaat atccgtcact tcaggatgta cgtcatccgt ggcgacagag
    12721 gtggtcgcag cggcggtaga tggggccatg atttgatccc aggccgcctg caacgctttc
    12781 atgttaaatt ccggctcgcc tttcggctgc agcagtatta acgccatatc attcgacaac
    12841 tgctgacgca gatcctgatt taacatctcc acagtcaggc tgttaaggaa atcctgccgc
    12901 agtttctgat actgctccgg cgcgatatcg acgacctgat tttgcaacga acgcatccgc
    12961 tgacccatca gaatatcggt atcagctcgt gcataggcgg caaacagttt ctgcagctcc
    13021 agtttctttt gcgccactaa cgcattgaac tcttcttccg gcagaccttt atcgcgaacc
    13081 ttcgccagtt cacgcgccac cagattaagg ttgctgttca gcttgtcgtt tggtgattcg
    13141 atgttgatgg cacactgcgc acgcagatac agcacacggc agtcaaatcc aagaccgatg
    13201 tctttgctgt tactggcgct taacgcttgc tgaacatgcc agaacagcgc ctcacgggcc
    13261 aggtccgcac gccagtagcg cagcagtgcg gctgattcac gaatcggctg ccacggcgta
    13321 tcccacatga tagataaccg gtcctgacgc accgcgtcag tcataatact caccgcttcc
    13381 gcacgcagcg gagaaagcgt cggcaccgga gccggtgttt cacgtttgcc tttcagttcg
    13441 ccaaacgttt tgttgatttg gtcgacgacc gagcgcgcat ccacgtttcc caccaccagc
    13501 agcgtcattg catccggggt gtaccatttc tgatagaaat ctttaatttt ttccgcttca
    13561 acgggttgtt tcagcggatc ggcaggatcg tgacctaaca gggttgatcc tttcagacga
    13621 taacgccacc agccctcttt agtatcggca ggccaggttg ccaccatgtc ctgactttgc
    13681 agcgcgtggt tgatggtttc tggcgtgata gtcagtttgc cagtggcatt tgccagataa
    13741 gagagcgcct ctttcagcaa atcgttacgg ttattgggca aactcagatt aaacagcgtg
    13801 gtgtcataag agacaattac cggcggcatc gggcgtttag ggtcgatccc ctgctgccac
    13861 aatgaacgcg cctgtgctgc gtcaaggcca ccgctttgcg ttagcgcaat acgagggatg
    13921 gcgtgactgt aaccgctctg ttgtgtactt tcggcgagcg aaccggtatt aaccagcagg
    13981 cgaatttcaa cacgatcgct gggacgctgg ggggtggtca gcacttgcca ctgtaaaccg
    14041 ttggaaagcg tcccctgttg ccatgctgga tcaggctgga gcgcatctgc ctgcacatag
    14101 ccagcagtgg ccatcatcag caaaccgccc gctaaaagtc gaatttttgt gccctgcatg
    14161 tgaacccctg atcaactatc ctggtaataa aaaaactgcc cgttgtcggt cagtctaaat
    14221 gacgttaaaa acacttcgtg ttagaccgca agaacatgaa aatgtcacgg aagaagtgaa
    14281 ataaacctga actcacccag gtggtaagtt caggcacagg ggtcaattat gcgcaaacac
    14341 ccgcactcgg ggaagggagt gcgggcataa gtgatgagat taagaggata attcgtgcgt
    14401 tttgccatcc ggcgcacgat tattcagcac atcgtccagt tttttgtggt ccagttcttt
    14461 cacccactta gcaacgacaa tggtcgctac gccgttaccg accaggttag tcagcgcacg
    14521 agcttctgac ataaagcggt cgataccgag gatcagcgcc agacccgcta ccggcaaatg
    14581 gcccaccgca gagagcgtcg ccgccagcac gataaagcca ctacccgtta cccctgccgc
    14641 ccctttagaa gaaagcagca acacgattaa cagcgtgatt tggtggacga tatccatctg
    14701 actgttagtg gcctgggcga taaacaccgc cgccattgtc aggtatatcg atgtgccatc
    14761 aaggttaaac gagtagcctg tcgggatgac cagccccacc accgatttac ggcagccgag
    14821 tttctccatc ttgtcgagca tacgcggcag cgccgactcg gaagatgaag tccccagtac
    14881 aatcagcagt tcttcacgga tgtagcggat aaatttgaag atactgaaac cagtcgcttt
    14941 agcgattgaa cccaatacca gcaccacaaa caggatacag gtaatgtaga aacagataat
    15001 cagctgcccc agttgcacca gtgtgccgac gccgtattta ccgatggtaa acgccattgc
    15061 cccgaacgca ccaataggtg ccagacgcat gatcatattg atgatgccga agatgacctg
    15121 cgagaaactt tcgatgacgt taaaaatcag ttggcctttg ctgcccagac ggtggagcgc
    15181 aaaaccaaac agtacggcaa acagcagcac ctgcagaatg ttaccgctgg caaatgcgcc
    15241 aatgacgctc gccgggatga catccataat gaaggcgaca atgccctggt ctttcgcctg
    15301 atcggcgtaa accgctaccg ctttcgcatc aagcgttgcc ggatcgacgt tcattccggc
    15361 accaggctgc acgacgttaa cgatgataag accaataatc agcgcgatgg tactgacaat
    15421 ttcaaagtaa agcagtgcga ctgcgccggt acgaccgacc gccttcatgc tttccatgcc
    15481 cgcaatgccc gttacgacgg tacaaaagat gacaggagcg atgatcatct taatgagctt
    15541 aacgaagccg tcgccaagcg gtttcatttg ctcgcctatt tcaggataga aatggccaag
    15601 gagaatacca atggctatcg ctgtcaggac ctgaaagtaa aggcttttaa agagagaggt
    15661 tttcataggg tgtcctttag taaaaccaca ggtcttgtaa ggttatgggg tacctgcggc
    15721 cttaaaataa cacccagaca acatcacaga aatgtacctg gatcataatt gaaacaaaaa
    15781 ggttaaaaag tttgagctgg ctcgcacaaa ccagcacttt ttaaagtttt gtaatcagtt
    15841 tggggtagct acttttcttc caggtaactc tcttcgaaga tttcaatagg gagtggacga
    15901 gcaaaaagga agccctgggc aataccaacg cccgcttttg ccagccagtc gcgttgtgct
    15961 tcagtctcca cgccttcggc aatcatttgt aagttcaggc tctgcgccag catgataatt
    16021 gcagcaatca tgctgctatc ttccggcaag ccttcaacaa acattttgtc gattttcagt
    16081 acgtcgattg gcaacgattt catatgctgc agctgacgca gccctgcgta gcccatgccg
    16141 aaatcatcca gcgccacccg aactccggca ttgcgcagcg gacggaggat tgccaccgca
    16201 gcatgagggt cgtcaatacg tcggctttct gtcacttcca gaatcagtgt tcccggctgg
    16261 atgcgatagc gggttaacag ttccagcata tccgccacca tattcgggtg catcagttgc
    16321 agcgcagaga ggtttaccga caagggcagc ataatgccgc gctcttgcca ggctgcaagc
    16381 aatcgacagg actcttccag cacccagtga ccgacggtaa ccatcagccc acagcactca
    16441 atgcgatcga ttaagccatc cggcaggtcc caactgccat ccggttgctg gatacgcagt
    16501 aacacttccg cactgaccag tttaccgctg gtcatctcga cctgtggctg taaccaaata
    16561 gcaaactgat gattttccag tgcattaagg atatcgctct cttccgtcaa ccgcttctgg
    16621 gcagcttcca tctgctgcgg atcaaagaac tgaatctgat tcttgccttt atggcgagcg
    16681 gtaaatgccg cagaaatagc gcgactgtaa agctgttcgg cggtgagatc gccgtagaac
    16741 atcgccacgc caatgctaca gtgcggacgg agttgaatac gttcaatcgg caggcgctcg
    16801 ctcatgatag tgagcacttg ctgacccaaa gtgattgcgt gccacggttc ctgtacaccg
    16861 ttggcaatga cagcaaagtc ataaccgcta atctgcgcga ggatcatacg tggcgacagt
    16921 accgatttga gtttttccac cagcgtcagc agcagaattt ctcgttgcgc ctctttcagc
    16981 acgcccgcag tatcacgcag agtttcacag gtgatgatca tcagcgcggt ggtttgttta
    17041 cgcgcgacaa cctgctccag catctccatc agcaaggctt tgttcggcaa atccgacacc
    17101 gggaagcgca tcgcattttc gttctgttct tcataatggc gctgcagcaa ttgctggttg
    17161 aggttgtaac tgcgcaccaa cataccgatt tcatcgtcct gatgcagacg cggtaatgcc
    17221 agttggtgac caacaagctc ctgggctggg atggcgttaa gttcgcgagc aatattgcgt
    17281 aacggatgca aaatcaggcg gttaatgcac cagctaatcg ccaccgtcaa tattaacgac
    17341 aaaagtaagt aaatggtcac taacgttgag agggtgctca tcacgaactt atacatacgg
    17401 aaggaatccg cctgtagcac cagataggca attggctgcg gatttgccgg acgttcgagc
    17461 gagtagacgc ccagcgagat ttgaaccggt agctcaaaca ggcgagtaac cattaccggc
    17521 accgggcgct ctggaataaa acttttacgc agcgcctgga actggttagg cagcactaca
    17581 tcggcacggc tgaccacgcc tgccggttta atgctggcaa gaatagcttc cgcttcggga
    17641 atatcgcctt tcaaaatggc tgaagataag ggttcgcgga cagagcgagc aatgctttcc
    17701 agttgcgtag ccgtgttata gcgattctgc tggaccagat ggaacagcaa aacggtgcaa
    17761 aaaataaaaa cgaacaccag gacaacggct gccaccattg ccatctgctt gattgttaac
    17821 gagcgactta cgcgcaaatt gtctcccccc ggaaatccca gattgccgcc cgagtatacc
    17881 cgatcgcagc ggcattaaga gaggcgctat ctgaaaactt accagtcggc gtagggtatc
    17941 agcggctgcg gcggtaaatc catgtcaccc tgccatccag cggcggaata acgtacatag
    18001 agcaggaaat ggctgggcgc gtaatctttc gcctgctgga tatcaattgc cgtaccgaca
    18061 aaccagttgg atgtgacgcg acgctcaagt aatgcccgcg ccgtatagcc aaagccctga
    18121 ctgctgccgc catcgttgga ttgccgcgca gcttcttcct gccagtcagt cgggatcaga
    18181 ttcatcagcg gataccgcgg catggttttg gtgcgcgaat gcgaccacga gccagacgcc
    18241 cccagctccc acgaccagtt ttccgtgcgc tcccgccaca tcaccggtat ggcaaacgac
    18301 aggtattcct gcggactgta gtaaccgccc tgaccgagtg agtagccact caggtctttg
    18361 tcgtaatgcc agatcatgtt gttcaggccg attgtgacgc ggcgattgtt ctggttgatg
    18421 accttatagt aatagcccgt catccagcgc acgcgccagt tatcttcgac atttttaccg
    18481 gttaactggt cgccactaag cgatgcccag acgccgtttg cttcaccttt atcgtagctc
    18541 aggcttagcc ccacgccgtc ggcacgcaca ccgccccatt ttttcccggt attgctcggg
    18601 gagtcttttt gcccaccaaa ggccagcaaa gaactggaga tgggccgacg gtgggcgtta
    18661 acggtgtaac ccagcggccc gatatcatcg ctgtaactga tgccgccgac cacatccacc
    18721 acgttgaagc ccatcggcgt ggtgccgata tcccagctcc agacgtcatt tcgccagccg
    18781 accgccacgc tggcaccgga atccgactgg ctgcggttgc cgctacagtc ctgtaatgta
    18841 caggtgcccc agttatcatc ccatttacca tcggcattag tggagaaact gccgacgttc
    18901 atattgacga aatcactgcg aaagaacatc cgcccgtcag aatacggcgc gtccacctgc
    18961 aacatggtag tgtgcgcttt cagatcggag taaccaccgg tgccgctcga tccccaataa
    19021 tcatgctcaa gggtgacgtt aagatcctgc tggcgataga ggtccgccgc atcgctgcgc
    19081 acgccgcgtt tcagccagtc atctttctcg tcgttacggg tcagtcgggt aaaggtgtcg
    19141 ttatcctgcg gacgcgtcgt agtcacaccg gatgcgacca tggcgtcttt gtaggtttcc
    19201 agcgcctgct ttggatcgcc cgcctgcgct tcaaacttcg caccgtcgcg cagcaccatc
    19261 gcgctttcca tcgacggtgg ctgagatttt gcctgcggga tcaacttatt aaacgtctgc
    19321 tgcgccgctg cggtatcgcc aagctgcgcc tgtgccagcg ccacgcgccg ctgtgtgttc
    19381 agcgaggcgt tatcggtagc gggcagtttc gccagctggc tacgtgccgc cgctttgtca
    19441 ccggcagcaa tatccacttc cgtcagacca agaatggcat cggcgttagt tggctcccgc
    19501 gtcaggacat tctgatacgc agcgcgggcg gcggtgtaat cacgtcgttg ttgcgcccag
    19561 tcagccagcg tgaggtcaat acgcgaggaa ggcggttgct ggtgcagcat cgcttccgct
    19621 tctgcctctt tgccgctttc tcgcaggcga ttagcggttt ccagcacctg atcgctttgc
    19681 agtcgattaa ccagctcctg aatattgctg ctccactgcg cacgcggcag gctgttgata
    19741 tgcgccagcg ccgctctgtc ctggtcatga ccagagaggt acagcccgta agcgtaaacc
    19801 tgctccgggt cgttcggctt ctgctgcgcc agattgcgca ttaacgtatc ggcctggctg
    19861 cgttgtccgg cctgccagag atcctgcgaa agtcggtaag taatccatac gctgccgggg
    19921 tccagcgcca gtcgttgccg ctgaagtgct gccgcctgcg cccatttgcc ctggttttcc
    19981 agtgcctctg cctgctgtgc cagacggtcg ttttgcaggc tgcgttcgat atcatcaatg
    20041 ctacgccgct gactggcaga gagtgaggcg ataaacgctt cagctttttc tggcgattgc
    20101 tggcggtaaa tatttgccag cccgcgcacg gcgttagtgt tgccgctgtc catacgcaag
    20161 gtctgctgat aataacgttc ggcggcggga taatcttttc gcgccatcgc cacatcgccc
    20221 agccccagca ctgcataact gtcggtgtta tcgacattac gcgcctgctg gaacaggcgt
    20281 tctgcccggt caggattatt ggctttcagc gcagcatcgc cctgctggat tgccagccag
    20341 tagcggttta ctttcagcag actgttccat ttgtcgttgt tgctgctgtg cgggtccagt
    20401 gcgagggctt tttccagatt cgccactgca ttggcgcgat cgcctttctg tgaatatgcc
    20461 tggcccagcg cccccagagc ctcactgtct ttcgggttcg cccgcactgc ctgttgtagt
    20521 tcgggaatgg ctttacccgc cataccagag tccaccgccg ctaaaccttg cgcacgagcg
    20581 cggaaagcag gatcggccag ctgtttttgc tgttctgcca gttgcgattg cgcagccgcc
    20641 acgctatcgc catcactaaa gatcgaaaga tattttttca gcgcctgcac gctggcatcg
    20701 ctgacgggca tgtctttaat ctgcccgtac cagattttag aggccccttc gcgcccggcg
    20761 ttcgatttcg ccatctgttc caggacggca aaaccttcgt cacggcgatc gctactaaac
    20821 agcaatagcg ccagattgtt ttgcaggccc gtattgcccg gtgcatccgc attgatgcgt
    20881 tttaactgat taatcgcttc gccacggcga gccggaattt tcgccaccgt actccagtac
    20941 tcgacagcaa tgtcaccttc cggcggcgca ccgttgaaca gcttgttgta actcgccaca
    21001 gcttcttccg catgaccggt agtcgcctgc aatcttgcct gttgcagtgc ctgacgacca
    21061 tccggcgtgg aaagtagcat cgtagtccgc gacgatttat acgcatttga actcggcgct
    21121 aactgcgaca gccgatcgag ctgtttttgc gcgccatcaa tatcgccctg acgtaacaaa
    21181 gaacggaaac gggcggcaac gacgtccggg ttattcggat caataagttc cagccgatac
    21241 aacgactgct gcaccaggtc ttcacgatgg gtcgcttcgc ctaaccgaac ttgctccagc
    21301 aactgttgct gagcggttgg tgctgcctcg accatcggca tgacggccag accgagggaa
    21361 agcgtgaata tgtttagtgt gaatttgcgc attcctggcc ccagtcaggt aataactcac
    21421 cttttgtcga gaagcggaaa cggtgttgat cccagccttg tccaaacagg gtcagcacat
    21481 agttgtaata ggcatcgctg ccgggaaagt tatcggccac gcgctggcgc tgaacggcct
    21541 gcgcatcacg gttttgtaaa aagggcagca tggcggcaga aaaaccgacc ggtcctttac
    21601 cctgcgcttt ccccgtagcc acatccactt tttccggcgg ataaccgttt ttctcagtga
    21661 atgtcgccat cggtttaaac cggttgagca tccgcgcttt ttgcggatcg ctgtcaggca
    21721 tcatgcctac ccacatgtaa acgcggatgg cgtcgtagct gctgatcaag gttttttcgg
    21781 cttttagctg ccagcctttg tctttctcat agcgcaccca gtctggcgaa aagcctttcg
    21841 gggcggtttc cagcaataaa cgttgattgg tttcgcgcag cgtggtccac ggcgcgccaa
    21901 agcgggtgaa atactgcgcc agcgtcggcg gcaggtagct ggggttaaaa cgccagctgt
    21961 tatcctctgc aaaacccact ttgcccggta acaacatgga acccagccca ggcaccgtca
    22021 ccacttcctc ccgcgcaata cgttttagca acgcgctgcc gatgtcggta taacgctgct
    22081 ctttccacaa acgccccgcc tccagcaacg accaggccat ccagacatca ccatcggagg
    22141 ccgaattgct gtccagcact tcccacttac tgttctcttt cttgccccac agccaggcgg
    22201 gcaaacgttc ttttaaagaa ccctgagcga gattgttctg cgtccagtcg agaatattat
    22261 cgaaagctgc acggtcgtta gccgccaggg caaagaacat gccgtaactt tgcccttcgg
    22321 aggtggtgat tttgcgcgcg tcgctggggt cgatgacgcg cccttcctga ctgatgtaat
    22381 cctttttaaa ctgctcccag gcaggccagg tacaggctgc ctgaacacta aaggcagcca
    22441 acagcagcat cgtcacgagt ccactacgca acacattcat cttcaattac tcgttatccg
    22501 ggttaagacg acgacgacta ataatacgca gcagacgcca cagtacccat gccagcaata
    22561 tcacactgat agccgccagc accgccagca gaatcggatg gtttgccagc gcataccaca
    22621 agcgctcgaa ccacggcaga tgacctacgt aataaacgtc gccaacacgt aggctgttga
    22681 taccggactc gcggatcacc gcgaccgaac cgaacatggt ggcgcgtttg ccgctatcgt
    22741 tcaccgcatc gttaagcatt tcataaccgc gtgggctatc tgccagcagc gcaatcacgc
    22801 tgcgctggtc gttatacggc gactggaagc caatcaccgc cgccatcgca ccggaagagg
    22861 tcagcgttga ctgggtttct gccgcgcgat cgctctcgtc cggcacaatg ccggggaacg
    22921 gggtctggcg catcggcgtt ttcacccagc tttcggtcgc ctgcaccaac aggtcgatct
    22981 gcttatcgtc tttcagtttg tccgggatac caccgatgat catgatgtcg gcatctttgc
    23041 cctgaatggt gctgccatca tcggtcaccg tcagattaat cgccgggaag cccgtctgtg
    23101 cgccgataaa accaacagta ttcagcaacg tttccatctg tgcttcgtta ggcgttttcg
    23161 gcatcacggt gatggtttgc gacagatccg ccatccggct gaacgggaag cccgcgttag
    23221 caaaggcgcg tagatccggc atcgggatga agtggtaata cttcgagaag tcgatggtgg
    23281 aatcgtcacc aatcaccaca tgattctgca ccggctggaa ggtaatacag ttatccaccg
    23341 aaccgcccgg catcgggttc atatactcaa agtcgaagcg cagctggttg gtcgcgccca
    23401 gtttcagcgc cggaatagag acatccgttt tgccatccag caaaccttgt aataccggaa
    23461 tccgaagcag caggcggttc gcctcctgtt tactgctcag gttgaaggat tgcaggaact
    23521 ggttattcag gctgatatcc atccgcgaac tgtctttcac cggcggcatg gtgtagcggt
    23581 aattaatatc catatcaatg ccggtactgc gcatcaggta gagatccggc ggcaggttta
    23641 gcgaaacgtt aatcgctgct ggctcaagac cgctggattg taactgttct tcataggttt
    23701 tcagttcgcc aaatgtgacc ggacgatcgg tacgtaccca gttcggcgca tcgtacggct
    23761 tacgcggtag cagcggtttc acttcattca ctaccacgct ttcaccacgg aacagaatgt
    23821 taccctgagc gatacctttc gctgcctgca acaggtcttt gtcgtcacga ccaaacacca
    23881 ccagcagttt gacgtaagga ttctgcggat ggttaatcat ctcaatcacc ggggctttta
    23941 ccgccggatg atcgcgcagg aagtccggcc gtttgtcgtt ggttgcaaag acaatggcat
    24001 tgcgatccgg cagttggtta tagagcaccg ggaagttctg cccacgccag ccagaacgcg
    24061 aaccaaacca cgaggcgaca atggcagatg cttgttgcag cccaacatcc ggcgcacccg
    24121 caaagaccat cggcaaggtg ttggtgcggt tatcacgcgg gtcaaagaat ggtaccggga
    24181 agtgtgacag gtcattcttc acattcaggg tctgataggt caaatccagt ccactgctcc
    24241 gcccaacatc cagccaaagc gtggtgctgg ccgggttttc gcacacgtcc tgataatggc
    24301 cgacaaactc cagccgcaca cggttgaagt cggtaataaa cagtgggtta atgggcattt
    24361 gcgccagcgt ttttttaccc aactgttctt tggtcactgg cagcacgccc atcagctcat
    24421 cattgagata aacctttaac tgcgactgga caggcagtaa cgatggcgat ggcgtgtatt
    24481 cgaggttgag catcgccttc gtcaccactt catcgctgcg cataccaaac tcaatgctgc
    24541 cgttcgggtt aatgccacgt agcaccatgc tgcccggcgg cggcgcaatt tgcgcaaagg
    24601 tcagcttcac atcacgcgaa ggaccgttct gcgccacgac tggcgcatca gcgccctgca
    24661 cgcccggcat cacttgcccc acctgcggat tttgttccgt ctgggcggct atagctggct
    24721 cagcattgat cagtggttgc gttgctggcg tcgcctgcgt catgaaagag gggaacgcac
    24781 tcatccccat agccactgca caaatccaga atagttttct tttcatcgcg ttatcatcat
    24841 tgttgagcca aagcctgatc cgatggttgt gccgtttcgc tccgctccgg gcggcgcgga
    24901 ataaacgata caacccagga aaccagagaa gtcagcacac ggaatatgcc cttcaccgaa
    24961 gaaggcgcaa actccgccag atggcggtag ccacggaagc cgagcttcag aatatccagc
    25021 agactttcca gcggcttatc ttccggatag ctgtcctgcc agagcgccca tgtatccgca
    25081 cgggcaaacg tacactgcac aaaatcgata tgttgctggg tggtgagcgg cattaatttc
    25141 agcccaactt cattacccat cacgcgcgcc acctgggtcg ggaagacgta ttcctgctga
    25201 ccgcgtttaa gcaacagatt cactttctgc ccttccagaa tctgcgcctg accgttgatc
    25261 ttgatcccca aaccaccgtc ggagaaatcc tgaacggtac acgagaagag gtgaccatct
    25321 tcgcgggcaa ttgccgcggg catcgtcatc tccacgcggt gcgatcggcg tacctgtttg
    25381 ctttctaccg ataccgcaac tgcgccgcca agaacaatca ggttgtagaa tacccacacc
    25441 atactgacga ccacggtgag catctcggtt ggcgggccat agaagtagcg ccagatgcct
    25501 accgcaacgc ccaccaggtt gagcagaaca aggaagatgt agggccgcga gatcacccag
    25561 tcgacgtact cttcttccac cagtccacct ttggcggtga cgttaaattt gcctttgtgc
    25621 gggttaatca gcgccaccag cgtcggtggt gcgatatacc acgccagcac cgtttcgtag
    25681 atttcactcc agaaagagtg gcgatatttg ccctggatct tggagttggt caggctggca
    25741 tggatcatat gcggcagcac gaacagggcg atcatcaacg ctggcgcata gatgatgtag
    25801 gcatgaagca gcaggaacgc cagcggcgca gtcaggaaga tcagccgtgg aatgcccgac
    25861 aagaagtgga acatggcgtt gacgtaacat agccgctgag caaacttcag ccctttaccg
    25921 gtgagcgggt tatcgagacg gaagatttgc accatcccgc gcgcccagcg aatacgctga
    25981 ccgatatgcg ccgacagact ttcggtcgcc agccccgccg cctgcggaat acgcatatat
    26041 gcagaggtgt agccacgacg gtgcaggcgc aaagaggtat gcgcatcttc agtcacggtt
    26101 tcgacagcaa tgccgccaat ttcatccagc ggcttacggc gaatcaccgc acaggaaccg
    26161 cagaagaaag tggcgtccca catatcgttg ccatcctgca ccagaccata gaacagcgtg
    26221 ccttcgttcg gtgttttacg aaaacgcccc aggttgcgtt caaacgggtc cggcgagaag
    26281 aaatggtgtg gggtttgcat catcgccagc tgtttctctt tcaggaacca gcccatggtc
    26341 atttgcagga acgatcgcgt tggtacgtgg tcgcagtcga aaatcgacac gaactcgcct
    26401 ttggcatatt tcagcgcatt gttgatgttg cccgctttcg catgttcatg agtggtgcgg
    26461 gcgatatatt tcacccccac gttttgcgca aactggcgaa actcttccct gccgccgtca
    26521 tcgaggatcc agatgttcag tttgtctttc ggccagtcga tacccagcga ggcgtaaatg
    26581 gtatttttca ccacgttgag atcttcgttg taagtcggga caaagatatc caccgacggc
    26641 cacagcgaca tatctttcgg caatggcacc ggctgacgat tcagcggcca tactacctgg
    26701 aagtagccga gcaccagcac aatccacgcg tacgtttcgg cgaagagcag aataagcccg
    26761 cacaccaggc tgaccggatc gtcccagttc agcgtagagg tgtaacgcca ccagatataa
    26821 cggcaagaaa cagtcagcga cagcacaatc aacattagcg ctgagaagcg ccccggcatc
    26881 cgccgtacga tcagcgctac cccccacagc agcatcagga agataaactg cgccagcggg
    26941 ttaaacggct gagtaacgca gattaacgcc agaatcagcg agaaggtgac gatgataccg
    27001 aggatcaacc gccgcgcccc ggcactcaaa tgaccgagct cttttttctc atcaagatgc
    27061 tgtgttttat ggctaacgcg ctcaggcagc tcgttcatcc attgatggta acgtccacga
    27121 atattctgca gacctgaaaa tgcccgcctg cgcggtttcg gcgtttcttt gcgcgatgta
    27181 ccgatcagta accagcatgt ttgaatgaga taacggaccg ggtccagcgg acgcggacgc
    27241 gaggcgttga tatgcggata caggttttta tgttctgcgc gaatacgctg ccagcgcggg
    27301 tgctccagcg gaataaaaat ccaggccagg atcatccaga aacagccgag cgtcgcgctg
    27361 aaagccgacg caccgtgacg acgataatcg cgataacgcc cgataagccg cgcgttgacc
    27421 ggcgggatta gcaaccaccg ggtcaggata ctcatgattt actcccgact ggcgttttca
    27481 gcccggaata gttcaacagg caccagttcg ccagcgtcag tatctcttca gcggccagcg
    27541 catcactgcg atattcacct actggttgct tagccgccaa gcattcagcc atcgcttcat
    27601 cacgatgaat gagcatcggc agtaatcggc gctggctttg caaccaaagc tggtaaatat
    27661 cgtcctgaac ctgactgcca atacggaagt cattaatcaa aatatgtgcg ccatccggca
    27721 gcgcttgctg atgcagtcgg atatggcagt tggcatccac attgacgatt gccagcgagt
    27781 gatcgcacaa actcagcagc tggtgggtta tctgcgaggc atcacgcggt aagtcgatta
    27841 aaatccactg gtaacgcccg ctggatttta gttgctgtaa gccggagcaa atatcgctca
    27901 gccgagtttg ccagtgctgt ggattttctt gttcttcaat ggataactga ccaaaaggca
    27961 gcaaatcgag ctgcgaggtg tagcgcaacc cagcgtcacg ccagtcctgg ccatccagca
    28021 tagctctggc ccagccctga cggtgggtaa aatcaacgtt aaatgacagg cgcaacaagt
    28081 tgtccgggca ggcatcgacc accaggacat tttctcccaa catttgtaat gaccaggcta
    28141 atgcggcggt gatggtt
//
LOCUS       DAZKFU020000038        27265 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZKFU020000038 DAZKFU020000000
VERSION     DAZKFU020000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.038"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..582)
                     /gene="yeeO"
                     /locus_tag="ACHOQQ_RS06475"
                     /old_locus_tag="pgaptmp_001295"
     CDS             complement(<1..582)
                     /gene="yeeO"
                     /locus_tag="ACHOQQ_RS06475"
                     /old_locus_tag="pgaptmp_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU4469570.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKEAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPL"
     gene            743..818
                     /locus_tag="ACHOQQ_RS06480"
                     /old_locus_tag="pgaptmp_001296"
     tRNA            743..818
                     /locus_tag="ACHOQQ_RS06480"
                     /old_locus_tag="pgaptmp_001296"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:776..778,aa:Asn,seq:gtt)
     gene            complement(856..1806)
                     /gene="cbl"
                     /locus_tag="ACHOQQ_RS06485"
                     /old_locus_tag="pgaptmp_001297"
     CDS             complement(856..1806)
                     /gene="cbl"
                     /locus_tag="ACHOQQ_RS06485"
                     /old_locus_tag="pgaptmp_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HGU4469571.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1908..2825)
                     /gene="nac"
                     /locus_tag="ACHOQQ_RS06490"
                     /old_locus_tag="pgaptmp_001298"
     CDS             complement(1908..2825)
                     /gene="nac"
                     /locus_tag="ACHOQQ_RS06490"
                     /old_locus_tag="pgaptmp_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGU4469572.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3151..3226
                     /locus_tag="ACHOQQ_RS06495"
                     /old_locus_tag="pgaptmp_001299"
     tRNA            3151..3226
                     /locus_tag="ACHOQQ_RS06495"
                     /old_locus_tag="pgaptmp_001299"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3184..3186,aa:Asn,seq:gtt)
     gene            complement(3282..4217)
                     /gene="ldtA"
                     /locus_tag="ACHOQQ_RS06500"
                     /old_locus_tag="pgaptmp_001300"
     CDS             complement(3282..4217)
                     /gene="ldtA"
                     /locus_tag="ACHOQQ_RS06500"
                     /old_locus_tag="pgaptmp_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4469573.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4279..5358)
                     /gene="cobT"
                     /locus_tag="ACHOQQ_RS06505"
                     /old_locus_tag="pgaptmp_001301"
     CDS             complement(4279..5358)
                     /gene="cobT"
                     /locus_tag="ACHOQQ_RS06505"
                     /old_locus_tag="pgaptmp_001301"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGU4469574.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5370..6113)
                     /gene="cobS"
                     /locus_tag="ACHOQQ_RS06510"
                     /old_locus_tag="pgaptmp_001302"
     CDS             complement(5370..6113)
                     /gene="cobS"
                     /locus_tag="ACHOQQ_RS06510"
                     /old_locus_tag="pgaptmp_001302"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGU4469575.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLMALL"
     gene            complement(6110..6655)
                     /gene="cobU"
                     /locus_tag="ACHOQQ_RS06515"
                     /old_locus_tag="pgaptmp_001303"
     CDS             complement(6110..6655)
                     /gene="cobU"
                     /locus_tag="ACHOQQ_RS06515"
                     /old_locus_tag="pgaptmp_001303"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGU4469576.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRKGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQVINTEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            6879..7013
                     /locus_tag="ACHOQQ_RS06520"
                     /old_locus_tag="pgaptmp_001304"
     CDS             6879..7013
                     /locus_tag="ACHOQQ_RS06520"
                     /old_locus_tag="pgaptmp_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGU4469577.1"
                     /translation="MEEISLVKVWLREAGAHNVLVTSAVNNNGVAELLALLHTEEGCR
                     "
     gene            complement(7114..7443)
                     /gene="tmaR"
                     /locus_tag="ACHOQQ_RS06525"
                     /old_locus_tag="pgaptmp_001305"
     CDS             complement(7114..7443)
                     /gene="tmaR"
                     /locus_tag="ACHOQQ_RS06525"
                     /old_locus_tag="pgaptmp_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGU4469578.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(7615..8673)
                     /gene="yeeA"
                     /locus_tag="ACHOQQ_RS06530"
                     /old_locus_tag="pgaptmp_001306"
     CDS             complement(7615..8673)
                     /gene="yeeA"
                     /locus_tag="ACHOQQ_RS06530"
                     /old_locus_tag="pgaptmp_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4469579.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRAVRK"
     gene            complement(8872..9345)
                     /gene="sbmC"
                     /locus_tag="ACHOQQ_RS06535"
                     /old_locus_tag="pgaptmp_001307"
     CDS             complement(8872..9345)
                     /gene="sbmC"
                     /locus_tag="ACHOQQ_RS06535"
                     /old_locus_tag="pgaptmp_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGU4469580.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(9464..10630)
                     /gene="dacD"
                     /locus_tag="ACHOQQ_RS06540"
                     /old_locus_tag="pgaptmp_001308"
     CDS             complement(9464..10630)
                     /gene="dacD"
                     /locus_tag="ACHOQQ_RS06540"
                     /old_locus_tag="pgaptmp_001308"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGU4469581.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            10839..12266
                     /gene="sbcB"
                     /locus_tag="ACHOQQ_RS06545"
                     /old_locus_tag="pgaptmp_001309"
     CDS             10839..12266
                     /gene="sbcB"
                     /locus_tag="ACHOQQ_RS06545"
                     /old_locus_tag="pgaptmp_001309"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGU4469582.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNLIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGNNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(12309..12536)
                     /gene="tsuB"
                     /locus_tag="ACHOQQ_RS06550"
                     /old_locus_tag="pgaptmp_001310"
     CDS             complement(12309..12536)
                     /gene="tsuB"
                     /locus_tag="ACHOQQ_RS06550"
                     /old_locus_tag="pgaptmp_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HGU4469583.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(12550..13608)
                     /gene="tsuA"
                     /locus_tag="ACHOQQ_RS06555"
                     /old_locus_tag="pgaptmp_001311"
     CDS             complement(12550..13608)
                     /gene="tsuA"
                     /locus_tag="ACHOQQ_RS06555"
                     /old_locus_tag="pgaptmp_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGU4469584.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASSLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(13788..15146)
                     /gene="plaP"
                     /locus_tag="ACHOQQ_RS06560"
                     /old_locus_tag="pgaptmp_001312"
     CDS             complement(13788..15146)
                     /gene="plaP"
                     /locus_tag="ACHOQQ_RS06560"
                     /old_locus_tag="pgaptmp_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGU4469585.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(15136..15198)
                     /gene="yoeI"
                     /locus_tag="ACHOQQ_RS06565"
                     /old_locus_tag="pgaptmp_001313"
     CDS             complement(15136..15198)
                     /gene="yoeI"
                     /locus_tag="ACHOQQ_RS06565"
                     /old_locus_tag="pgaptmp_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGU4469586.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(15413..16342)
                     /gene="yeeY"
                     /locus_tag="ACHOQQ_RS06570"
                     /old_locus_tag="pgaptmp_001314"
     CDS             complement(15413..16342)
                     /gene="yeeY"
                     /locus_tag="ACHOQQ_RS06570"
                     /old_locus_tag="pgaptmp_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4469587.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANCAIAGVYAKSDDGKSAI"
     gene            complement(16388..17212)
                     /gene="yeeZ"
                     /locus_tag="ACHOQQ_RS06575"
                     /old_locus_tag="pgaptmp_001315"
     CDS             complement(16388..17212)
                     /gene="yeeZ"
                     /locus_tag="ACHOQQ_RS06575"
                     /old_locus_tag="pgaptmp_001315"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4469588.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            17639..17689
                     /gene="hisL"
                     /locus_tag="ACHOQQ_RS06580"
                     /old_locus_tag="pgaptmp_001316"
     CDS             17639..17689
                     /gene="hisL"
                     /locus_tag="ACHOQQ_RS06580"
                     /old_locus_tag="pgaptmp_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGU4469589.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    17665..17788
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            17835..18734
                     /gene="hisG"
                     /locus_tag="ACHOQQ_RS06585"
                     /old_locus_tag="pgaptmp_001317"
     CDS             17835..18734
                     /gene="hisG"
                     /locus_tag="ACHOQQ_RS06585"
                     /old_locus_tag="pgaptmp_001317"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGU4469590.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            18740..20044
                     /gene="hisD"
                     /locus_tag="ACHOQQ_RS06590"
                     /old_locus_tag="pgaptmp_001318"
     CDS             18740..20044
                     /gene="hisD"
                     /locus_tag="ACHOQQ_RS06590"
                     /old_locus_tag="pgaptmp_001318"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGU4469591.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSTLASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            20041..21111
                     /gene="hisC"
                     /locus_tag="ACHOQQ_RS06595"
                     /old_locus_tag="pgaptmp_001319"
     CDS             20041..21111
                     /gene="hisC"
                     /locus_tag="ACHOQQ_RS06595"
                     /old_locus_tag="pgaptmp_001319"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGU4469592.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLMAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            21111..22178
                     /gene="hisB"
                     /locus_tag="ACHOQQ_RS06600"
                     /old_locus_tag="pgaptmp_001320"
     CDS             21111..22178
                     /gene="hisB"
                     /locus_tag="ACHOQQ_RS06600"
                     /old_locus_tag="pgaptmp_001320"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGU4469593.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            22178..22768
                     /gene="hisH"
                     /locus_tag="ACHOQQ_RS06605"
                     /old_locus_tag="pgaptmp_001321"
     CDS             22178..22768
                     /gene="hisH"
                     /locus_tag="ACHOQQ_RS06605"
                     /old_locus_tag="pgaptmp_001321"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGU4469594.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            22768..23505
                     /gene="hisA"
                     /locus_tag="ACHOQQ_RS06610"
                     /old_locus_tag="pgaptmp_001322"
     CDS             22768..23505
                     /gene="hisA"
                     /locus_tag="ACHOQQ_RS06610"
                     /old_locus_tag="pgaptmp_001322"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGU4469595.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSAEDVAALLEAGVARVVVG
                     STAVKSPDMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            23487..24263
                     /gene="hisF"
                     /locus_tag="ACHOQQ_RS06615"
                     /old_locus_tag="pgaptmp_001323"
     CDS             23487..24263
                     /gene="hisF"
                     /locus_tag="ACHOQQ_RS06615"
                     /old_locus_tag="pgaptmp_001323"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGU4469596.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDEGRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            24257..24868
                     /gene="hisIE"
                     /locus_tag="ACHOQQ_RS06620"
                     /old_locus_tag="pgaptmp_001324"
     CDS             24257..24868
                     /gene="hisIE"
                     /locus_tag="ACHOQQ_RS06620"
                     /old_locus_tag="pgaptmp_001324"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGU4469597.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPKALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGETAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(24965..25942)
                     /gene="wzzB"
                     /locus_tag="ACHOQQ_RS06625"
                     /old_locus_tag="pgaptmp_001325"
     CDS             complement(24965..25942)
                     /gene="wzzB"
                     /locus_tag="ACHOQQ_RS06625"
                     /old_locus_tag="pgaptmp_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGU4469598.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETAEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(26089..27255)
                     /gene="ugd"
                     /locus_tag="ACHOQQ_RS06630"
                     /old_locus_tag="pgaptmp_001326"
     CDS             complement(26089..27255)
                     /gene="ugd"
                     /locus_tag="ACHOQQ_RS06630"
                     /old_locus_tag="pgaptmp_001326"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGU4469599.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         7098 a         7163 c         6547 g         6457 t
ORIGIN      
        1 tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaatta gagtgatggc
       61 agctgctgga taactgagta ccgtcagctc cagataagtc aacgccagtg ctttaacttc
      121 tgtcgtggca tcacccgcga cgaaatcaat aatttgctcg ccaaaatgat gaataagcgt
      181 tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc
      241 tcgtcgtcga tcccgcttac cgagactaaa tgccacaacg acagtagtac caagatcgat
      301 agcagcaaaa aaagccataa tgaccatatt gaagctgtcc gccaatccca cgccggccat
      361 cgcttctttt cccagccagc tgaccagaaa agtgctcaga acccccatca acaggacaca
      421 ggcattctcc atgaagatag gaacagcaag cggggttatc tcgcgccaga acaacacttt
      481 gtagctcttg cgtttagcgt gccagcgagt gccgtgaaca acctggcgta aagcagagga
      541 gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaata atgagggaga
      601 atcagcaaag ctgcaaagat tttcgccaac aaattgtctg caaatgcaac aaactgttga
      661 tagaaacggc aaacagttgg ggaatttaaa aatcgggttt gacaaaagat ttttcgccgt
      721 taagatgtgc ctcaacagcg attcctctgt agttcagtcg gtagaacggc ggactgttaa
      781 tccgtatgtc actggttcga gtccagtcag aggagccaaa tttctgaaaa gcccgctttt
      841 atagcgggct ttttgctata tctgataatc aatttcctct tcactgtttt ccatcacctg
      901 ccgcttgata tcctcaactg aaagtcctgc attacaaagt tccagaaagc gccagacata
      961 gttacgctga agttgtcctc gcttcagtcc caaccagaca gtattagcat caaaaagatg
     1021 ccgcgtatcc aggcggatta aattcttttc ctcttgttca ccgctggatt gttcggcaac
     1081 tattccgatc ccaagcccaa gagcaacata ggttttaatg acatcagaat cctgcgcact
     1141 taatacaata tctgccagca aacctttgcg ggcaaatgcg tcatcaatac gtgagcgccc
     1201 cgtaatcccc tgtcggtaag tgattaacgg ccacttcgct attgactcca gcgtcaatgg
     1261 tgtaatttgc gtcaagggat gatcgagtgg aacaagcaaa ctatggtgcc aacgaaacca
     1321 cgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac caatatcagc
     1381 ttcaccatta tgcaacaacg tcgcaatttc ctgtggcgtc ccctggatta gctcgagccg
     1441 aacctccggg aaaagttcgc gaaaagcttt aatgacctct ggcaagctat aacgtgcctg
     1501 agtatgcgtc gttgcaatag tgagaacgcc agacgtatcg ttggtaaaca ggtctgcaag
     1561 ccgacgaaca ttactggctt cattcagaat acgttctgca atgaccagta atgctttgcc
     1621 cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca aatatttcga tgccaagttc
     1681 atcctccagt tcccgaatat gacggctgac gcctgactgt gaggtaaaaa gcatattcgc
     1741 aacctctgtc aggttgtaat cctgacgtgc agcttcgcgg attatcttta gttgttggaa
     1801 attcacggta aactccgggc agttcagata ttccgttatt gttaaagtct aacgcccggc
     1861 ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacgctta gctcaccaat
     1921 tgccactgcc ttttttccat cactggagaa ctaatcactg acattaacaa ctctttcact
     1981 gcctgtgcct gtggcgataa gttcgctctg gcgggtaaat ttaatgacaa agagagactc
     2041 atggaaggcg tggtaatacg tgacatccac ccatttactg cgccacataa cgaacgcgcg
     2101 gccgattcgg gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt aagcgtggca
     2161 atagactcaa tttcaccaat gacttttgcc gtgagtcgcc gtagggaaaa cgcctcatca
     2221 acacgaagtc taacagcgct gtaatcactg gggagaaaga ggttcatttg tgcaatagca
     2281 ttaacatcaa cgctttgccc cgggcaatct tgagttccta ccaggaaaag atcttctttc
     2341 agcaaagcct gactggatac cccagccaca ggggaatgct cataaatcac cgccatatcg
     2401 agttggtgat ttatcagttt ttcgttaagc actgcaccgc tattttcatg aagatagata
     2461 acgatctccg ggaattcagc gcgaaccgct tgtaataagg gcatggtgat agatgacgca
     2521 gcggttcctg gcgcaaagcc aatcgagact tgccccgata atgcctgacc aacgttatgc
     2581 accgccagtt gggcctgctc acactgacgt aaaatggccc gcgcatgggt atagagaatt
     2641 tttccggcgt ctgttggcgt aacgccccgt tttgtgcgaa tcaaaagttg ttgatttaac
     2701 tcaccttcca gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg caatacttca
     2761 gcagcctgag tcaggctacc aatatctaca atttttacga agtatttcag gcgtctgaag
     2821 ttcatgttgc ctccggtttt tgagaatcgg cccaagtgcc gccattactt acaaccagac
     2881 tgcaagatgc ttgccagttt tattttggcg ttgatgtaca agctaaccaa ctgtcaaata
     2941 agagattatg atagattcgt cattggctct tttcaccagc agtcgcgttc ccctgcccca
     3001 taaaaggaga gtatgcacca cgatggttca taaccaataa gattgaaagc tcaccacttt
     3061 gttgaaattg acagcaaaca aacaaaaaaa tgcatttcac cctttgacat caccatgcac
     3121 tgccattaat atgcgccccg ttcacacgat tcctctgtag ttcagtcggt agaacggcgg
     3181 actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt caaaaaagcc
     3241 tgctttctag caggcttttt gctttctaat taccaacgct cttaaaacat ctgtcttgaa
     3301 ccagaactaa tttgcacagg cattcccgat cgacgttgca acgcagcatt tgcgcgattc
     3361 acatcaactt cttgcccgtt gataaacgcc cgcaaagatg gggttaccgg caatggcact
     3421 tttcggtcag actcatattc tgcacgattg cgcgacagcg gctcatgaac ttccagccag
     3481 ttcgagccat ctggttcagt ggtgtatttt actggctggt cgataatttg cacacgcgtc
     3541 ccaacaggaa cattatcaaa cagatatttg atatcgtcat tgcgcagacg aatacagccc
     3601 tgacttaccc ggagcccaat accaaaattg gcattggtac cgtggatggc atacaacctg
     3661 ccaatataaa tcgcgtacag ccccatggga ttatcgggcc ccgcaggaac aaatgcgggc
     3721 aaactctccc ctcgtttcgc atattcgcgc cgagtgttcg gcgttggcgt ccaggttgga
     3781 gcttcttgtt tacgttcaac ggtagtcacc cagttacgcg gggtttctcg cccagcctgg
     3841 ccgataccaa taggaaagac ttccacagta ttactgtctg gtgggtagta ataaagacgc
     3901 atctcagcga cgttaaccac aatcccttta cgaacagtgg cgggcaaaat cagttgctgc
     3961 ggaatggtga gttgcgagcc agacttcggc aagaaaacat cagcgcccgg gttcgcttcc
     4021 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     4081 tgatcaggaa cagttacagt aaacgactgc cccactaaac ggctaccctc tggaggtaat
     4141 ggataagtta ccgccaggct agtatggctg gcaaaaagca gagcaaatga gcaaagaata
     4201 tttacacgac gcatcatgtc cctttcctat gtcgcgaaag ctatccgtta agtatagctt
     4261 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     4321 attactggca gcaagttcgc ccatgttgtt gtatatcgca caggcagctt cgatgatggg
     4381 catcgccaga gctgcgccac tcccctcacc taaacgcatc tccatattga gataaggctc
     4441 cagccccaaa tgcgacagcg ctatacgcgc ccctttttct gccgacaggt gagaaggaat
     4501 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcagcag cataagaaag
     4561 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     4621 tccgaccaaa tcaaatccac ccacttttgc cagaacatca acgccatcct gcggatttgg
     4681 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     4741 caggtttgcg ccaatcccaa ccacttcttc aggatcccgg ccagtgattg tgctgactat
     4801 tgctgctgcc ggtgtcgtgt ttgccatccc cagttcacct acaccaaaca gcgtgacacc
     4861 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt cagcctgacg
     4921 gcgactcatt gccggagctg aagcaatatt gccgctacct cgtgcgacac gcatgttgat
     4981 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtgga cgttagcacc
     5041 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     5101 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     5161 cataaccagt accgcttttt tgcccacatg cggtatgcca ttcaaccccg gcattcctgc
     5221 cagttgtatg gcaagcgcct ccagccttcc aaggctacca acaggtttga gtaacccgtc
     5281 aatatgccgt tgtgcacgcg acatagcggc aggatcgata gcagggatcg tattcagtaa
     5341 atcggcaagt gtttgcatgt cacgtccctt tatagcagtg ccatcaggaa gaccagttca
     5401 ccaagctcta tggctgctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     5461 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     5521 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     5581 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     5641 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     5701 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     5761 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     5821 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     5881 cccccggtca tcagcgccag cacaagcacg ctaaacagtg ccgccagtgg tacgccacac
     5941 catgcctgca gcaccatgaa aaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     6001 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     6061 ggtacaggca gacgcgtaat aaatgagagc atcgcccaaa ataatttact catttgattt
     6121 ttactccaat acccgaaacc accagccata cttcatttgc cgcagcggcc agctgctgat
     6181 ttacccgccc ggcaatatca cgaaaatgac gtgccagacg actctccggc acaatcccca
     6241 ttcccacttc gttagtcact aatacaacct ttgcggggca acgttggcag gcagcaatca
     6301 acgactgaat ctcagtatta atcacctgtt ccatcgcctg ataatcccat tcatcagggt
     6361 ctttatcgcc gccataatca aacaacagat tagtcaccat tgtggtaacg cattccagca
     6421 acacagcttc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccaacgct
     6481 ccaccgtgcg ccagtgcgcc gggcggcctt tccgatgatg ctctatccgt gcagccatct
     6541 catcatcaag gatttgggag gtggcgatat acagaacctg tgaagagtcc ccaataagcg
     6601 cctctgcgtg gcgacttttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     6661 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     6721 tgaccgcaaa acatttacac gattaatact gcgcgtaata taattaaaaa tccagcattc
     6781 tcaatccatt acgaaagaag atggcattgt ggaatagcca gacttcctcg tgttaacgtc
     6841 cgatcattgg agtagtcacc aaagcagatt tagccagcat ggaagaaata tctttggtga
     6901 aagtttggtt acgggaagcc ggagcgcaca acgtgttagt aaccagtgcg gttaataaca
     6961 atggagttgc agaactttta gccttgctgc atacagaaga aggctgtcgc taatttcgac
     7021 aggttctcag atgagaatga tgactttgat acacagaaaa gaatcaatga gcaatgaggg
     7081 ttgccgggca accctcattg aataaaacag gaattacttc gcttcgccgt ttttcatttc
     7141 gcccatagct ttcagctttt tggagatatc gcggcgttct ttggagagct cggcattttt
     7201 gatgatgtaa tcgtcaacac gatcttcata gtcacctttc atgctggcga tgatgccctg
     7261 gattgcttca acgctcatcc ccggcttgat gtaatcgctc aggttgtcca gcagcaggac
     7321 gcgcttctgg ttgtcacgga tctttttctc aacgtcctga atttcacgtt gcagtttgtt
     7381 cttacgacgg aacagacgaa caaattccag tacgtcctgg aatgaaggct tggtagtttc
     7441 catttttata cccctgataa tgtgagagtc ggattcgttt aagcaaccgc tattcgttag
     7501 ggccaacatt actcatagct gccgcagatg acaatgcttt tatccctttt cactatcata
     7561 ccctttatcc ttgctgaatc gaagcagcag caagatgatt ctgaagttca ggaattattt
     7621 gcgcacggcc cggcaaatca gattcgatag caactcaagc tgatgtgccg tttccatgtt
     7681 cagccacaca taaccataga ttggtgtttc cacaaccttc aggtcatggt ggttattgag
     7741 caactgacgc agctcttcca cagcatcgtt caatttttcc gtattggcaa aaaccggctg
     7801 cggattacct tcgtacagcg catgaacaag gctcagcagt atttgctgca tcatgtgctg
     7861 ggtatcacga agtttttgcg cgttcaataa cacgaaatgg ctggggcgcg tggcccagta
     7921 cgcattgatt tgcaactcca gcatacaaac cagattgcga ttaattgtct ggataccttc
     7981 atatatcgat tttggaatac gggtttcttt gctggcgggc gcaatcaacc cacgcatttt
     8041 cacggcatcg gtcaggagtt tttgtagatg gctttccaga cgtgggcgtt cgagtaagtt
     8101 cggtgagaat gcagattgat agacccgatt atactcggtc agacttttcg ccagttgaat
     8161 gcgccagtgg atgaacgccc gttgtggcca gataccggta aacaacattg ccagtaaaga
     8221 gccgaggatc acatcgccgc ttcgccataa cgccgtatca atttcacctg tcggggaacc
     8281 aacaacaatt gccagcgtca ccccaatcaa taaaccttga tacggtttct tgcccagcgc
     8341 cagccaaccg caaaggaaca tggccgccgc gcaccagact aacatcagcg gtaacgagat
     8401 taactccagt tgcagagcga taagacctaa aatcgaaccc aacaccgtac cgccaatacg
     8461 ttcgaaggcg cgagggacaa cgttacccca gaacgagatt ggccccataa tcaccaccat
     8521 ggtgaccagc ggccaggtgc tttccgggat agtaaacagg cggataatga gaaaagtgag
     8581 caggaatgcc agcgcgaccc gagtaccatg cacaatgcgg taatggcggt atacccggat
     8641 ttcaaacggg cttaatgact tatcggcacg cacgcagaaa ctccgtaatg aagattaaaa
     8701 gcgctcataa ctcatctgtc ggccagaagg cgcgcctctg aattatccga cggacaaacc
     8761 gggaacagtg taatttcccg gcaggagcga aaaaattgaa aggcgcatct tactcttttc
     8821 gctttcaaaa aaaagagtgg tcatcgcgtt aacacaccgc cctgagatga attagtgatg
     8881 ttttggctgc accgcaacat acatttcgat atcccagtac ccatcttccg cgccattgtt
     8941 caaataaacc tcgaagcagg gctttggtaa catttcataa gcactgtcct gcaagaggct
     9001 attaaagaac tgataccagg gtttagcaaa atcatcaccg actacacgag ctaccgccac
     9061 cgcatactga ccacctgaaa tttctgtcag aatgacgccc tcactgtttt cggggagcgt
     9121 aaagtaaccc ggcaccgtca cgacggtgtc gcagcgtaat ttttcggcgg gtgtttcatc
     9181 tggattgtcg taatagacag caacccattc cttcggcaca atatttttgc tatctaccca
     9241 catcatcaac tgctcaaaac ctttctttac cgtctgttcc cacgggccaa cgagatggaa
     9301 acctgcaacg gtacgtttat cttcctgctt aatctcgtag ttcatgacgc ctccattaac
     9361 actgttttta tatacagtat agttgcaata ttaaaaccac aaggaatgag tgttgattat
     9421 gcgagcagac tcgcactcct gccagtctgc tgcaaaagaa aggtcaggcc ttatggtgga
     9481 aataatcact caggcgggaa aacatgccgc cttccccgac agattccagg gtaaccagcg
     9541 gccagtgcgc cacctgttta tcacggtcgt aaagttcaat ttcccctacc cgctgatggg
     9601 cgctaattgg cgcggtgagc tctttaccat caagggtata tttggctttg atatgtggaa
     9661 tttcggcttt cggtagcacc atccagaact cttgttccgt tcccaggtcg atattttctt
     9721 tatcgccata ccagatgcgt tccgttccga cctttttccc acggtgcaaa atttgcaccg
     9781 tagtaaagtt ttgttgcccc caacgtagta attttcttgc ctcttcctca cgaccttttg
     9841 cactgtcagc ccccattacc acggcaatga gacgacgctg cccatctaca gccgaagcaa
     9901 tgagattaaa cccggcacca gaagtatgac ccgttttcag gccgtcaaca ttcatggttt
     9961 tatcccacaa caacccgtta cggttttgct gggtgatacc gttccaggtg agacttttct
    10021 cactgtacat atgataaaac tcgggctcgc cgtggatgat agcgcgagaa agtacagcta
    10081 aatcataagc cgagctatgc tgacctggcg catccaggcc atgcactgtt tcaaaatgcg
    10141 tatccttgag atgcagcttc tcggcatagt tgttcatcat ttcaacaaac tgccgttgcc
    10201 caccggcaat atagtcagcc agtgcaacac aagcgtcatt tccggaatcc acaattaaac
    10261 cacggcttaa atcacgtacc gatacgcgat cgccctcttt caaaaacatc agtgaagaac
    10321 cgacaaacac cggattatct ttcgcccacg catcgcgccc cacggtgaca atatcgtctg
    10381 gcgtaatgcg atgactatcg atagcgcgat ccacgacata acccgtcatc agctttgtca
    10441 ggctggcggg attgcgctgt tgatgctcat tacccgcggt gaggatctgt ccggtggtgt
    10501 aatccatcag tacccaggac ccggcatgaa tctctggagg ctgaggtgaa aaaggaatgt
    10561 tttccgccgc aaaaccagac gataagttaa aaacgaacaa agaagcagca ataataagac
    10621 ggcgtttcaa cagcaaaccc tcaggagttt caaatagctg ttctttttac ggaaatactt
    10681 atgaactggc tggaataaag tgcaaaaaaa tgtgactacc ctctcatttt tatctgacat
    10741 gatctgttgc cactcgctgc caaattgtgg cgctaaagct gattagcacg gtgatatttg
    10801 atactctggc agacagcaga aataacggat ttaacctaat gatgaatgac ggtaagcaac
    10861 aatctacctt tttgtttcac gattacgaaa cctttggcac gcaccccgcg ttagatcgcc
    10921 ctgcacagtt cgcagccatt cgcaccgata gcgaattcaa tctcatcggc gaacccgaag
    10981 tcttttactg caagcctgcg gatgactatt taccccagcc aggagcagta ttaattaccg
    11041 gtattacccc gcaggaagca cgggcgaaag gagaaaacga agccgcgttt gccgcccgta
    11101 ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat gtgcgtttcg
    11161 acgacgaagt cacacgcaac attttttatc gtaatttcta cgatccttac gcctggagct
    11221 ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt tatgccctgc
    11281 gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt cgccttgagc
    11341 atttaaccaa agctaatggt attgaacata gcaacgccca cgatgcgatg gctgatgtgt
    11401 acgccactat tgcgatggca aagctggtaa aaacgcgtca gccacgcctg tttgattatc
    11461 tctttaccca tcgcaataaa cacaaactga tggcgttgat tgatgttccg cagatgaaac
    11521 ccctggttca cgtttccgga atgtttgggg catggcgcgg caataccagc tgggtggcac
    11581 cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggtggatttg gcaggagaca
    11641 tttcgccatt actggaactg gatagcgaca cattgcgcga gcgtttatat accgcaaaaa
    11701 ccgatcttgg caataacgcc gccgttccgg ttaagctggt gcatatcaat aaatgtccgg
    11761 tgctggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga attaatcgtc
    11821 agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgc gaaaaagtgg
    11881 tggcgatatt cgcggaagcc gaaccgttta cgccttcaga taacgtggat gcacagcttt
    11941 ataacggctt tttcagtgac gccgatcgcg cagcaatgaa aattgtgctg gaaaccgagc
    12001 cgcgtaattt accggcactg gatatcactt ttgttgataa acggattgaa aagctgttgt
    12061 tcaattatcg ggcacgcaac ttcccaggga cgctggatta tgccgagcag caacgttggc
    12121 tggagcaccg ccgccaggta ttcacacccg agtttttaca aggctatgca gaagagattc
    12181 agatgctggc gcagcagtat gccgacgata aagagaaagt ggcgctgtta aaagcgcttt
    12241 ggcagtacgc ggaagagatc gtctagtttg aagcctccgc cgctgttacg gcggaggaca
    12301 atgttgggtc aggctttttg aacggtgatg ctccacgcag catcgccaat ctgctgataa
    12361 tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt tgcctgggtg
    12421 caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc tgcttttgct
    12481 tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat cgccatcttt
    12541 acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata
    12601 gacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa cccagccctg
    12661 ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac ctgcaatact
    12721 ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt gtgcatcagc
    12781 tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgacccca cgaagatccc
    12841 taacaccaag aaaacgcccc agttaatgta tttcacgtca cctgcgacca gaaattgcag
    12901 aatattggcc gttggggaag tgattcctaa cccaaacatg cgtccggttg cttcactcaa
    12961 gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg gatgccagcg
    13021 tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg cgactttcag
    13081 ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca aaacggcaac
    13141 caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag tactgtagtg
    13201 ctgcaaggtt tgatttaaac tactggcatg tggagaacgc atcaccgcac tcatcaccat
    13261 ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat accaggtccc
    13321 ggtggcacat ccacccgcca gaacaattcc cagcccgaag atatacccac ctataacagt
    13381 accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa
    13441 aacccccacg ctttgtaccg aaattgcaat cagcagggcg taaaacatgc gattattttt
    13501 cgcgatatac atatcgcgaa aaccacctgt caggcaaaaa cgcccacgtt gcatgacaaa
    13561 tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa attaattaac
    13621 cagatgaatg ttaatgagga aattattcat gactggtgga atgcagacca ataaccaaat
    13681 tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa taaaaaaaac
    13741 cggagccaaa tgttcattcg actccggttt attattagaa aagatgatta tgctacgtct
    13801 tcgtactgcg gaaccgggtt acggaagctc ttggtgacgc aagccaggta aatcagaccg
    13861 atagcagccc agatcagacc cagaaccatt gagctttctt ccaggttaac ccacagcgca
    13921 ccaacggtca gcgcaccaca catcggcagg aacagatact ggaagtgatc tttcagcgtc
    13981 ttattacgct tctcacggat ccagaactgc gagatgaccg acaggttaac gaaggtgaac
    14041 gccaccaacg caccaaagtt gatcagcgcc gtagccatca ccaggtcgaa gttgattgcc
    14101 agcagggcaa tcgcgccaac caggatgatg ttcatcgccg gagtacgcca tttcgggtgt
    14161 acataaccga agaagctttt cgggaatacg ccgtcacgac ccattacgta catcagacgc
    14221 gctacgcctg cgtgcgccgc cataccggac gccagtacgg taatggtgga gaagatcagc
    14281 gcgccaacct ggaacgcttt acccgcaacg tacagcatga tttcaggctg tgacgcatcc
    14341 ggatctttaa agcgagagat atccgggaag tacagctgca ggaagtaagt tgcaaagatg
    14401 aagatcatgc cgccgatcag cgcggtcagg aaaatcgcac gcgggatcac gcgctctgca
    14461 tctttggttt cttccgacag gttgctgatg ccgtcaaagc cggtaaagga gaagcacagg
    14521 attgtcgccc cggtaatcat cgggataaca tgcgcatcgc cagaccagaa tggacgagtg
    14581 ctcgccagcg taccagcgcc ttcaccttca aatacgccat aaacaaccat gcccagaatc
    14641 accgcgatca gcactacctg caacacgacg attacagtgt tgaagttcgc tacggatttc
    14701 agactacgca ggttaaaggc ggtcataaag gccaccagcg ccaccacaaa catccacgat
    14761 gggatggaag gcaccagagc ttcaaaatag attttcgcca gcagaatgtt gatcatcggc
    14821 gcgaacagat agtcgagcag agaagaccaa cccaccataa agccgacagt cgggctaatg
    14881 gatttctggg cgtaagtata tgcagagcca gcagaaggat agcggcgaac cagcttcccg
    14941 tagctcagag ccgtaaacag gatcgcaatc aatgcgaacg cataggctgt cggcacatga
    15001 ccatccgtga ggcctgaaac gataccaaaa gtatcaaaca gcgtcatcgg ctgcatatag
    15061 gcaagaccca tcattacaac cggaactaac gtaagcgttt tacgcaattc cacgcgagag
    15121 gtgtttggag taacgttatg cgacatggtc attctccttt acggtgataa ccgtcgcgta
    15181 agcaaaaaat tgccccattt ttttggattc ctcagcgaca acaactgtcg atttttagta
    15241 aatatctatc cggtacgaag cccggcctct tggtatgaat gattggtttg aagcaaaaaa
    15301 taaccgacgc tgatgaaacg tcggttttta gtcatttttt gacagcggcg cattgtgcct
    15361 aaatgggggg gaaatgacaa gagaatgaga ggcttgtcat aataattttt ctttaaatgg
    15421 ctgattttcc gtcatcagat tttgcataaa caccggcgat cgcacaattt gctaaaattt
    15481 catcccgcca ccagccactg gcaagtcctg cggtctgttc attccagcca atccagacgg
    15541 gttcaaagag agtttgggcg accactttct tctcgattaa cgcgccacta tcgagaaaac
    15601 gttgcgccag ataacggggt aaatagccgc agccgaggcc gctaatttgc agctccagct
    15661 tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc gagaagttgt gaggccgtag
    15721 aagcgcctgc ctgcgcagtg tcccccacca caatcgcccg atatcgttta atgatacgac
    15781 ggtttaacgg ttcttcttcc tgggctaacg gatgatgagg cgcgaccgca aagacttgtt
    15841 ccagatcgcc caaccgcgag aaaccaaact cactggaaga tggtggctca tgcatcgcgc
    15901 caacaataat atccgctcgc ccctgagtca gggcatccca ggaaccaccg agtacgccat
    15961 tgataaactt caggcgcgtc acgctatgat gttgataaaa agcttcaatc agtggcgcaa
    16021 gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag ctcgttttcc cagccttcat
    16081 gcagtttgat cgcctgcttt tccagttccc gcacggtatg cagaacttcc cgccctttct
    16141 ctaataacat ttttccggtg cgggtgaatt tagcgcggtg accgctacga tcaagcaatt
    16201 gaatattaag gtcgctttcc agcttgtgaa cggtgtaact cagagcggac ggcgtcttgt
    16261 agagtttggc cgacgccgcc gcaaaactgc cttctttttc taaggcatcg aggatcatca
    16321 acacatccag cagtggtttc atacacgccc ccttgcagtg catgcggcgt cctgctggcg
    16381 tgatcgttta ctccagcggc attaccagcg gatcgggata ctggtattca aatcccagtt
    16441 cattacaaat ccgactgcca tcaataatct tacctttgcc gctgtccaga ctatttctga
    16501 actgcggagg ttccagcccc agtaaacggg ccatctgcgg atagaaaaca ttacgcgcag
    16561 ggtgagctgg cgcacatata ttatagatgt gtccgccttt aggtgcctgt aacaacagag
    16621 tgatagcgcc aataacatct tctaaatgga ctaaattaac accatgttca ccatcaggcg
    16681 cggtttttcc ggcaaagaag cgtccgggat gacgccccgg tccgaccagg cccgcaagac
    16741 gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca gtcttcgagt tcttctaata
    16801 ctcgtccact gttggttacc ggattacgcg gtgtagtttc tttcaccgtg ccttgcgcat
    16861 cgccatagac agatgttgag ctggtaaaaa taatgcgggg aatacgatga gccagcgcgc
    16921 tatccactaa ctcttgtacc gcttgtaaat agaactcatc gccggggccg ctacgacgtg
    16981 ccggaagcgt aatgaccagc gcatcggcat ccatcagggc atccagatcg tcagaatcgc
    17041 aaactaactc aggctccatg cgaagcagat agctatcaat gccactcatt cgggccgctt
    17101 cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca gcctcgcgct gaaagtgaca
    17161 tcgccagcgg catgcctaac caccctaacc cgacaattgc gacctttttc atccgttatc
    17221 tcctgataca ccttagatct ataaggctac gctagcgtat caaaactgac aattcattct
    17281 atgaatgaaa tcgaaacggt tagcaaatat accattgcct gatgcgctcc gcttatcagt
    17341 cctacaaaaa catcaatata ttgaatttag gcaacatttg tagaccagat atggcattca
    17401 cgccgcatcc ggcaggaaca aagcccactt tagttacaga acacaatgtt cttgagggtt
    17461 ttaatgacat attttcacga ttactttttt aacctagtca ctcttagccc cctcctttcc
    17521 ccgctcattc attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta
    17581 acgtgaaagt ggtttaggtt aaaagacatc agttgaataa acattcacag agacttttat
    17641 gacacgcgtt caatttaaac accaccatca tcaccatcat cctgactagt ctttcaggcg
    17701 atgtgtgctg gaagacattc agatcttcca gtggtgcatg aacgcatgag aaagcccccg
    17761 gaagatcacc ttccgggggc ttttttattg cgcggttgat aacggttcag acaggtttaa
    17821 agaggaataa gaaaatgaca gacaacactc gtttacgcat agctatgcag aaatccggcc
    17881 gtttaagtga tgactcacgc gaattgctgg cgcgctgtgg cattaaaatc aatcttcaca
    17941 cccagcgcct gatcgcgatg gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg
    18001 acgacattcc cggtctggta atggacggcg tggtagatct tgggattatc ggtgaaaacg
    18061 tgctggaaga agagctgctt aaccgtcgcg cccagggtga agatccacgc tactttaccc
    18121 tgcgtcgtct ggatttcggc ggctgccgtc tttcgctggc aacgccggtt gatgaagcct
    18181 gggacggccc gctctcctta aacggtaaac gtatcgccac ctcttatcct cacctgctca
    18241 agcgttatct cgaccagaaa ggcatctctt ttaaatcctg cttactgaac ggttctgttg
    18301 aagtcgcccc gcgtgccgga ctggcggatg cgatttgcga tctggtttcc accggtgcca
    18361 cgctggaagc taacggcctg cgcgaagtcg aagttatcta ccgctcgaaa gcttgcctga
    18421 tccagcgcga tggcgaaatg gaagaatcca aacagcaact gatcgacaaa ctgctgaccc
    18481 gtattcaggg tgtgatccag gcgcgcgaat caaaatacat catgatgcac gcaccgaccg
    18541 aacgtctgga tgaagtcatc gccctgctgc caggtgccga acgcccaact attctaccgc
    18601 tagcgggtga tcaacagcgc gtagcgatgc acatggtcag tagcgaaacc ctgttctggg
    18661 aaacgatgga aaaactgaaa gcgctgggtg ccagttcgat tctggtcctg ccgattgaga
    18721 agatgatgga gtgatcgcca tgagctttaa cacaatcatt gactggaata gctgtactgc
    18781 ggagcaacaa cgccagctgt taatgcgccc ggcgatctcc gcttctgaaa gcattacccg
    18841 cactgttaac gatattctcg ataacgtgaa agcacgcggt gatgacgccc tgcgggaata
    18901 cagcgcgaag tttgataaaa ccacggttac cgcgctgaag gtgtctgctg aggaaattgc
    18961 cgccgccagc gaacgcctga gcgacgagct aaaacaggcg atggcggtgg cagtaaagaa
    19021 tattgaaacc ttccacactg cgcaaaaact gccgccggta gatgtagaaa cgcagccagg
    19081 cgtgcgttgc cagcaggtca cgcgcccggt cgcttcagtt gggttgtata ttcctggcgg
    19141 ctccgccccg ctcttctcaa cggtattaat gctggcgact ccggcgcgta ttgcgggctg
    19201 taaaaaagtg gtgctgtgct caccgccgcc gattgccgat gagatccttt atgcggcgca
    19261 gttgtgcggt gtgcaggacg tgtttaacgt cggcggcgca caggccattg ccgcgctggc
    19321 gtttggtacg gaatctgtgc cgaaagtgga caaaatcttc gggccaggaa acgcctttgt
    19381 caccgaggca aaacgtcagg tgagccagcg tctggacggt gcggcgatcg atatgcccgc
    19441 aggcccgtcg gaagtgttgg tcattgccga cagcggcgca acgccggatt tcgtggcttc
    19501 tgatttgctt tctcaggctg aacacggccc ggactcacag gtgattttac tgacgcccgc
    19561 tgctgatatg gcgcgtcgcg ttgccgaagc tgtcgaacgc cagttggcag aactgccgcg
    19621 tgccgaaacc gcccgtcagg cactgagcgc cagccgcctg atcgtgacta aagatttagc
    19681 gcagtgcgta gagatctcca accagtacgg cccggagcac ctgatcatcc agacccgcaa
    19741 cgcccgcgat ctggtcgatg gcatcaccag cgccggttca gtatttcttg gtgactggtc
    19801 accggaatcg gcaggtgatt acgcctccgg caccaaccac gttctgccga cttacggtta
    19861 caccgccacc tgttccagcc tcgggctggc ggatttccag aagcggatga ccgtgcagga
    19921 actgtcgaaa gaaggctttt ctacgctggc ttcaaccatt gaaacactgg ccgccgccga
    19981 gcgcctgacc gcccataaaa atgccgttac tttgcgtgtt aacgccctta aggagcaagc
    20041 atgagcaccg tgactattac cgatttagcg cgtgaaaacg tccgcaacct gacgccgtat
    20101 cagtcggcgc gtcgtctggg cggtaacggc gacgtctggc tgaacgccaa cgaatacccc
    20161 acagccgtgg agtttcagct tactcagcaa acgctcaacc gctacccgga atgccagccg
    20221 aaagcggtga ttgaaaatta cgcgcaatat gcaggcgtaa aaccggaaca ggtgctggtc
    20281 agccgtggcg cggacgaagg tattgaacta ctgattcgcg ctttttgcga accgggtaaa
    20341 gacgccatcc tctactgccc gccaacgtac ggcatgtaca gcgtcagcgc tgaaaccatt
    20401 ggcgtcgagt gccgcacagt gccgacgctg gaaaactggc aactggactt acagggcatt
    20461 tccgacaagc tggacggcgt aaaagtggtc tatgtttgca gccccaacaa cccaaccggg
    20521 caactgatca atccgcagga ttttcgcacc ctgctggagt taacgcgcgg taaggcgatt
    20581 gtggttgccg atgaagccta tatcgagttt tgcccacagg catcgctggc tggctggctg
    20641 gcggaatatc cgcacctggc tattttgcgc acactgtcga aagcttttgc tctggcgggg
    20701 cttcgttgcg gatttacgct ggcaaacgaa gaagtcatca acctgctgat gaaagtgatc
    20761 gccccctacc cgctctcgac gccggttgcc gacattgcag cccaggcgtt aagcccgcag
    20821 gggatcgtcg ccatgcgcga acgagtggcg caaattattg ctgaacgcga atacctgatg
    20881 gccgcactga aagagatccc ctgcgtggag caggttttcg actccgaaac caactacatt
    20941 ctggcgcgct ttaaagcctc cagcgcagtg tttaaatctt tgtgggatca gggcattatc
    21001 ttacgtgatc agaataaaca accctcttta agcggctgcc tgcgaattac cgtcggaacc
    21061 cgtgaagaaa gccagcgcgt cattgacgcc ttacgtgcgg agcaagtttg atgagtcaga
    21121 agtatctttt tatcgatcgc gatggaaccc tgattagcga accgccgagt gattttcagg
    21181 tggaccgttt tgacaaactc gcctttgaac cgggcgtgat cccggaactg ctgaagctgc
    21241 aaaaagcggg ctacaaactg gtgatgatca ctaatcagga tggtctggga acacaaagtt
    21301 tcccgcaggc ggattttgat ggaccgcaca acctgatgat gcagatcttc acctcgcaag
    21361 gcgtgcagtt tgatgaagtg ctgatttgtc cgcacctgcc cgcagatgag tgcgactgcc
    21421 gtaaaccgaa agtaaaactg gtggagcgtt atctcgctga gcaagcgatg gatcgtgcca
    21481 atagttatgt gattggcgat cgcgcgaccg acattcaact ggcggaaaac atgggcatta
    21541 atggtttacg ctacgaccgc gaaaccttga actggccgat gattggtgag caactcacta
    21601 aacgagaccg ttatgcccat gtagtgcgca acaccaaaga gacgcaaatt gacgtccagg
    21661 tgtggctgga tcgtgaaggt ggcagcaaga ttaataccgg cgttggcttc tttgatcaca
    21721 tgctggatca gatcgccacc cacggcggtt tccgcatgga aatcaacgtc aaaggcgacc
    21781 tctatatcga cgatcaccac accgtcgaag ataccggcct ggcgctgggc gaagcgttaa
    21841 aaatggctct tggcgacaaa cgcggtattt gccgctttgg ttttgtgctg ccgatggacg
    21901 aatgccttgc ccgctgcgcg ctggatatct ctggtcgccc gcacctggaa tataaagctg
    21961 agtttaccta ccagcgggtt ggcgatctca gcactgaaat gatcgagcac ttcttccgtt
    22021 cgctctctta caccatgggc gtgacgctcc acctgaaaac caaaggtaaa aacgatcatc
    22081 accgtgtaga gagcctgttc aaagcctttg gtcgcaccct gcgccaggcc atccgcgtgg
    22141 aaggcgacac cctgccctcg tcgaaaggag tgctgtaatg aacgtggtga tccttgatac
    22201 cggctgcgcc aatttgaact cggtgaagtc agccattgcg cgccacggtt atgaacccaa
    22261 agtcagccgt gacccggacg tcgtgttgct ggccgataaa ctatttttac ccggcgttgg
    22321 cactgcacaa gcagcgatgg atcaggtgcg tgagcgcgag ctgtttgatc tcatcaaagc
    22381 ctgtacccaa ccggtgctgg gcatctgctt agggatgcaa ctgctggggc ggcgcagcga
    22441 agagagcaac ggcgtcgact tgctgggcat cattgacgaa gacgtgccga aaatgaccga
    22501 ctttggtctg ccgctgccgc atatgggctg gaaccgcgtt tacccgcagg caggcaaccg
    22561 cctgtttcag gggattgaag atggcgcgta cttttacttt gttcacagct acgcaatgcc
    22621 agtcaatccg tggaccatcg cccagtgtaa ttacggcgaa ccattcactg cggcggtaca
    22681 aaaagataac ttctacggcg tgcagttcca cccggagcgt tctggtgccg ctggcgctaa
    22741 gttgctgaaa aacttcctgg agatgtgatg attattccgg cattagattt aatcgacggc
    22801 accgtggtgc gtctccatca gggcgattac ggcaaacagc gcgattacgg taacgacccg
    22861 ctgccgcgct tacaggatta cgccgcgcag ggtgccgaag tgttgcatct ggtggatctg
    22921 accggggcaa aagatccggc taaacgtcaa atcccgctga ttaaaaccct ggtcgcgggc
    22981 gttaacgttc cggtgcaggt tggcggcggc gtgcgtagcg cagaagacgt ggcggcgtta
    23041 ctggaagcgg gcgttgcgcg cgtggtggtc ggctccaccg cggtgaaatc accagatatg
    23101 gtgaaaggct ggtttgaacg tttcggtgcc gatgccttag tgctggcgct ggatgtccgt
    23161 attgacgagc aaggcaacaa gcaggtggca gtcagcggct ggcaagagaa ctcgggcgtt
    23221 tcactggaac aactggtgga aacctatctg cccgtcggcc tgaaacatgt gctgtgtacc
    23281 gatatctcgc gcgacggcac gctggcaggt tctaacgtct ctttatatga agaagtgtgc
    23341 gccagatatc cgcaggtggc atttcagtca tccggcggca ttggcgacat tgatgatgtg
    23401 gctgccctgc gtggcactgg cgtgcgcggc gtaatagttg gtcgggcatt actggaaggt
    23461 aaattcaccg tgaaggaggc catcgcatgc tggcaaaacg cataattcca tgtctcgacg
    23521 ttcgtgatgg tcaggtggtg aaaggcgtac agtttcgcaa ccatgaaatc attggcgata
    23581 tcgtgccgct ggcaaaacgc tacgctgaag aaggcgctga cgaactggtg ttctacgata
    23641 tcaccgcttc cagcgatggc cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag
    23701 tgatcgacat tccgttctgt gtggcgggtg ggattaaatc gctggatgac gccgccaaaa
    23761 ttctttcctt tggcgcggat aaaatttcca tcaactcccc tgcgctggcg gacccgacgt
    23821 taattactcg cctggcggat cgatttggcg tgcagtgtat tgtggtcggt attgatacct
    23881 ggtacgacgc tgaaaccggt aaatatcatg tgaatcaata taccggcgat gaaggccgca
    23941 cccgcgtcac tcagtgggaa acgctcgact gggtacagga agtgcaaaaa cgcggtgccg
    24001 gagaaatcgt cctcaacatg atgaatcagg acggcgtgcg taacggttac gacctcgaac
    24061 aactgaaaaa agtgcgtgaa gtttgccacg tcccgctgat tgcttccggt ggcgcgggca
    24121 ccatggaaca cttcctcgaa gccttccgcg atgccgacgt tgacggcgcg ctggcagctt
    24181 ccgtattcca caaacaaata atcaatattg gtgaattaaa agcgtacctg gcaacacagg
    24241 gcgtggagat caggatatgt taacagaaca acaacgtcgc gaactggact gggaaaaaac
    24301 cgacggactt atgccggtga ttgtgcaaca cgcggtatct ggcgaagtgt taatgctggg
    24361 ctatatgaac ccgaaagctc tggacaaaac catcgaaagc ggcaaagtca ccttcttctc
    24421 gcgcactaaa cagcgactat ggaccaaagg cgaaacgtcg ggcaatttcc tcaacgtagt
    24481 aaatattgcg ccggactgcg acaacgacac gctactggta ctggcgaatc ctatcggacc
    24541 aacctgccat aaaggcacca gcagctgctt cggcgaaact gctcaccagt ggctgtttct
    24601 gtatcaactg gaacaactgc tcgccgaacg caaatctgcc gacccggaaa cctcttacac
    24661 tgccaaactg tatgccagcg gcaccaaacg cattgcgcag aaagtgggcg aagaaggcgt
    24721 ggaaaccgca ctggccgcga ctgtacatga ccgctttgag ctcaccaacg aggcgtctga
    24781 tttgatgtat cacctgctgg tgctgttgca agatcaggat ctggatttaa cgacggtgat
    24841 tgagaacctg cgtaaacggc atcagtgagt tgcggggtaa gcggatgcaa tattgttgtc
    24901 gcatccggca aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
    24961 aagattactt cgcgttgtaa ttacgcagag cgttacgccc cagcacaatc cccgcaccaa
    25021 ccatgccgcc cagcagcacc gccagaatca aggtgattgc ctttttcgga ctatcgcgac
    25081 gaataggtaa cgtcggtttc atcacataac ggtaagcatg aatatcaaga tcatcaactt
    25141 ttaagctttc gatatccagc aggttttgac gagtctgata gtagtttggt gagaacacca
    25201 acggacgggt cgcctcatgc ttaatcatcg actccagcgc ttcgctcccc aaaaggaaca
    25261 acgtatcttg tgtcacatct tcagtctgct gaacctgtgg ctttgtcacc tgcgcctgat
    25321 tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc tcctgcgcga
    25381 ctacttcctg agttttcaga gaatcctgca agtttttcat acgcagcgcg atgttgtctt
    25441 tcaggtcttt ttccagttct tcgtttacct ggtcgtcaac ttgctggata tacttcgcca
    25501 gctgcttctg cgcaccttca gctgtttcac ccacataaga aactgctaaa ggtaacgact
    25561 ggtttttaac ggtaggctca atggtcagct tctctgcttc ttcctggtta tccagcgttt
    25621 ccgctaatgc tgagaatgca gtgctgtaac gtccgataag cgaagcctga atgtcggaga
    25681 ctttcggtac agccgatccg tagatcacat tcatcgcgtt ggtataacca gcgatttgcc
    25741 ccacatcagg ttgagtaacg atagctgtgg aagtccactt ttctttggca acggccagat
    25801 accctatagc cagggcaata gcaacaatga cagagatgat aattgtcatc ttgccgcgcc
    25861 acaactgcac cagtaaatca atcaaatcaa tctgttccgg gtcatggttt tgcccagaaa
    25921 cattattatt ttctactctc atcattaccc taacggacga aataatttta agtcgccata
    25981 gtttagcgat aatcgccatg aatgctatag gataaatgat aaaaataatg aattattaca
    26041 aagggaacat aagagaatca gggcgaaaat cgccctgata acaggatgtt agtcactgcc
    26101 aaagagatcg cgggtgtaga ctttgtctgc cacatcctta agctcttctg ccatacggtt
    26161 ggaaataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca ggcgagagtt
    26221 gaagaatgag tcttctttca ttaccggttc gtaaatgatt acttcaacgc ctttcgcctt
    26281 gatacgcttc ataatcccct gaatggaaga cgcacggaag ttatctgacc cgctcttcat
    26341 aatcagacga taaataccca ccacttgcgg tttgcgtgac aaaatggcat cggcaataaa
    26401 gtctttacgc gtgcggtttg catcgacaat tgccgagatc aggttattcg gcacagactg
    26461 gtagttcgcc agtaactgct tggtatcttt cggcagacaa taaccaccat aaccaaacga
    26521 cggattgttg taatggttgc caatgcgtgg gtcgagacaa acgccttcga taatctggcg
    26581 agtattcagc cctaaacttt ctgcatagct atccagttca ttaaagtatg ctacgcgcat
    26641 cgccagatag gtattagcaa acagtttaat cgcttctgct tcggttgaat cggtgaacag
    26701 ggtcgggata ttttgcttaa tcgcgccttc ctgtaataac gcagcaaaac gttctgcgcg
    26761 ttccgaacgc tcaccgatta caatacgtga cggatgaagg ttatcgtaaa gagctttacc
    26821 ttcacggaga aattccggag agaaaataag gttatcgata cccagacgtt ctttaattga
    26881 tttggtgaaa cctaccggaa ttgtagattt aatcaccatt accgcgttgg gattaatttc
    26941 tgtcacatcg cgaatgaccg cttccacgct tgaggtatta aaataatttg ttttcgggtc
    27001 ataatcggta ggcgtggcaa taataacgta atcagcattt ttatacgcgt catacttatc
    27061 tgtcgtagcg cggaaattga gatctttagt cgccagatac tcttcaatct ccttatcaac
    27121 aatcggtgac tgcttcttgt taagcatgtc aactttggcc tgaacgatat ccagtgcaac
    27181 cacttcgtgg ttttgcgcaa tcagaatacc atttgaaaga ccaacataac ctgttcctga
    27241 aattgttatt ttcatagtcg tttag
//
LOCUS       DAZKFU020000039        26807 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZKFU020000039 DAZKFU020000000
VERSION     DAZKFU020000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.039"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..924
                     /gene="yieE"
                     /locus_tag="ACHOQQ_RS06635"
                     /old_locus_tag="pgaptmp_001327"
     CDS             43..924
                     /gene="yieE"
                     /locus_tag="ACHOQQ_RS06635"
                     /old_locus_tag="pgaptmp_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGU4469600.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1129..2598
                     /gene="lysP"
                     /locus_tag="ACHOQQ_RS06640"
                     /old_locus_tag="pgaptmp_001328"
     CDS             1129..2598
                     /gene="lysP"
                     /locus_tag="ACHOQQ_RS06640"
                     /old_locus_tag="pgaptmp_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGU4469601.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2890..4869
                     /gene="cirA"
                     /locus_tag="ACHOQQ_RS06645"
                     /old_locus_tag="pgaptmp_001329"
     CDS             2890..4869
                     /gene="cirA"
                     /locus_tag="ACHOQQ_RS06645"
                     /old_locus_tag="pgaptmp_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HGU4469602.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4901..5737)
                     /gene="yeiG"
                     /locus_tag="ACHOQQ_RS06650"
                     /old_locus_tag="pgaptmp_001330"
     CDS             complement(4901..5737)
                     /gene="yeiG"
                     /locus_tag="ACHOQQ_RS06650"
                     /old_locus_tag="pgaptmp_001330"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4469603.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5995..6663
                     /gene="folE"
                     /locus_tag="ACHOQQ_RS06655"
                     /old_locus_tag="pgaptmp_001331"
     CDS             5995..6663
                     /gene="folE"
                     /locus_tag="ACHOQQ_RS06655"
                     /old_locus_tag="pgaptmp_001331"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGU4469604.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6680..7837
                     /gene="yeiB"
                     /locus_tag="ACHOQQ_RS06660"
                     /old_locus_tag="pgaptmp_001332"
     CDS             6680..7837
                     /gene="yeiB"
                     /locus_tag="ACHOQQ_RS06660"
                     /old_locus_tag="pgaptmp_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGU4469605.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDTQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFTQFDR
                     LGLLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            7979..9019
                     /gene="galS"
                     /locus_tag="ACHOQQ_RS06665"
                     /old_locus_tag="pgaptmp_001333"
     CDS             7979..9019
                     /gene="galS"
                     /locus_tag="ACHOQQ_RS06665"
                     /old_locus_tag="pgaptmp_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGU4469606.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9299..10297
                     /gene="mglB"
                     /locus_tag="ACHOQQ_RS06670"
                     /old_locus_tag="pgaptmp_001334"
     CDS             9299..10297
                     /gene="mglB"
                     /locus_tag="ACHOQQ_RS06670"
                     /old_locus_tag="pgaptmp_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGU4469607.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10358..11878
                     /gene="mglA"
                     /locus_tag="ACHOQQ_RS06675"
                     /old_locus_tag="pgaptmp_001335"
     CDS             10358..11878
                     /gene="mglA"
                     /locus_tag="ACHOQQ_RS06675"
                     /old_locus_tag="pgaptmp_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGU4469608.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11894..12904
                     /gene="mglC"
                     /locus_tag="ACHOQQ_RS06680"
                     /old_locus_tag="pgaptmp_001336"
     CDS             11894..12904
                     /gene="mglC"
                     /locus_tag="ACHOQQ_RS06680"
                     /old_locus_tag="pgaptmp_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGU4469609.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(13147..14382)
                     /gene="preA"
                     /locus_tag="ACHOQQ_RS06685"
                     /old_locus_tag="pgaptmp_001337"
     CDS             complement(13147..14382)
                     /gene="preA"
                     /locus_tag="ACHOQQ_RS06685"
                     /old_locus_tag="pgaptmp_001337"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGU4469610.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14376..15614)
                     /gene="preT"
                     /locus_tag="ACHOQQ_RS06690"
                     /old_locus_tag="pgaptmp_001338"
     CDS             complement(14376..15614)
                     /gene="preT"
                     /locus_tag="ACHOQQ_RS06690"
                     /old_locus_tag="pgaptmp_001338"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGU4469611.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCIAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MVADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(15808..16047)
                     /gene="yeiS"
                     /locus_tag="ACHOQQ_RS06695"
                     /old_locus_tag="pgaptmp_001339"
     CDS             complement(15808..16047)
                     /gene="yeiS"
                     /locus_tag="ACHOQQ_RS06695"
                     /old_locus_tag="pgaptmp_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGU4469612.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(16050..16769)
                     /gene="sanA"
                     /locus_tag="ACHOQQ_RS06700"
                     /old_locus_tag="pgaptmp_001340"
     CDS             complement(16050..16769)
                     /gene="sanA"
                     /locus_tag="ACHOQQ_RS06700"
                     /old_locus_tag="pgaptmp_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGU4469613.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16919..17803)
                     /gene="cdd"
                     /locus_tag="ACHOQQ_RS06705"
                     /old_locus_tag="pgaptmp_001341"
     CDS             complement(16919..17803)
                     /gene="cdd"
                     /locus_tag="ACHOQQ_RS06705"
                     /old_locus_tag="pgaptmp_001341"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGU4469614.1"
                     /translation="MHPRFQTAFAQLAENLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17933..18628)
                     /gene="yohK"
                     /locus_tag="ACHOQQ_RS06710"
                     /old_locus_tag="pgaptmp_001342"
     CDS             complement(17933..18628)
                     /gene="yohK"
                     /locus_tag="ACHOQQ_RS06710"
                     /old_locus_tag="pgaptmp_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGU4469615.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPMILAVMG"
     gene            complement(18625..19023)
                     /gene="yohJ"
                     /locus_tag="ACHOQQ_RS06715"
                     /old_locus_tag="pgaptmp_001343"
     CDS             complement(18625..19023)
                     /gene="yohJ"
                     /locus_tag="ACHOQQ_RS06715"
                     /old_locus_tag="pgaptmp_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGU4469616.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            complement(19154..20062)
                     /locus_tag="ACHOQQ_RS06720"
                     /old_locus_tag="pgaptmp_001344"
     CDS             complement(19154..20062)
                     /locus_tag="ACHOQQ_RS06720"
                     /old_locus_tag="pgaptmp_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4469617.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            20189..21547
                     /locus_tag="ACHOQQ_RS06725"
                     /old_locus_tag="pgaptmp_001345"
     CDS             20189..21547
                     /locus_tag="ACHOQQ_RS06725"
                     /old_locus_tag="pgaptmp_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4469618.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            21559..22587
                     /gene="gtdA"
                     /locus_tag="ACHOQQ_RS06730"
                     /old_locus_tag="pgaptmp_001346"
     CDS             21559..22587
                     /gene="gtdA"
                     /locus_tag="ACHOQQ_RS06730"
                     /old_locus_tag="pgaptmp_001346"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HGU4469619.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            22603..23304
                     /locus_tag="ACHOQQ_RS06735"
                     /old_locus_tag="pgaptmp_001347"
     CDS             22603..23304
                     /locus_tag="ACHOQQ_RS06735"
                     /old_locus_tag="pgaptmp_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU4469620.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            23313..23957
                     /gene="maiA"
                     /locus_tag="ACHOQQ_RS06740"
                     /old_locus_tag="pgaptmp_001348"
     CDS             23313..23957
                     /gene="maiA"
                     /locus_tag="ACHOQQ_RS06740"
                     /old_locus_tag="pgaptmp_001348"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HGU4469621.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            23972..25165
                     /locus_tag="ACHOQQ_RS06745"
                     /old_locus_tag="pgaptmp_001349"
     CDS             23972..25165
                     /locus_tag="ACHOQQ_RS06745"
                     /old_locus_tag="pgaptmp_001349"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HGU4469622.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            25325..26275
                     /gene="dusC"
                     /locus_tag="ACHOQQ_RS06750"
                     /old_locus_tag="pgaptmp_001350"
     CDS             25325..26275
                     /gene="dusC"
                     /locus_tag="ACHOQQ_RS06750"
                     /old_locus_tag="pgaptmp_001350"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGU4469623.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSHDIARAIQAIDIDKLR"
     gene            complement(26663..26746)
                     /gene="yohP"
                     /locus_tag="ACHOQQ_RS06755"
                     /old_locus_tag="pgaptmp_001351"
     CDS             complement(26663..26746)
                     /gene="yohP"
                     /locus_tag="ACHOQQ_RS06755"
                     /old_locus_tag="pgaptmp_001351"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGU4469624.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         6378 a         6724 c         7172 g         6533 t
ORIGIN      
        1 gattatatat ttataattaa tctttataag tggtaagcga ctatgcacat caccctccgg
       61 cagttggaag tttttgcaga agtattgaaa agtggatcaa ccacccaggc gtcggtgatg
      121 ctggcgttgt cgcaatcagc agtgagcgca gccttgaccg acctggaagg gcagcttggc
      181 gtgcaactgt ttgatcgcgt ggggaaaaga ctggttgtta atgaacacgg gcggctgctc
      241 tatccgcgtg cgttggcact gcttgaacag gcggttgaaa tcgaacaact gtttcgcgaa
      301 gacaacggcg cgattcgtat ctatgccagt agtaccatcg gtaactacat tctgcctgca
      361 gttatcgccc gttatcgcca tgattatccg cagttgccga ttgaacttag cgttgggaac
      421 agccaggacg tgatgcaagc ggtgctggat ttccgcgttg atattggctt tattgaagga
      481 ccgtgccaca gcactgaaat catttctgaa ccatggctgg aagacgagct ggtggttttc
      541 gccgcgccga cttcgccgtt ggcccgtggt ccggtcacct tagaacagct ggccgctgcg
      601 ccgtggatcc tgcgtgaacg cggctccggc acgcgggaga ttgtcgatta tctgttgctg
      661 tcacatttac cgaagtttga gatggcgatg gaattaggta actccgaggc aatcaaacat
      721 gcggtgcgtc atgggttggg aattagttgc ctgtcgcgac gggtgattga agaccaattg
      781 caggcaggca cattaagtga agttgcggtc cctctgccgc gcctgatgcg tacgttgtgg
      841 cgtatacatc atcggcaaaa acacctttcc aacgcgctac ggcgctttct ggactattgc
      901 gatcccgcga atgtcccgcg ttaagttgct gtacaagaac atgctggtgc tgtgtcgatt
      961 tcgtgacgca gcgccttcag catgcattcg ccagaaaaga gattggctgc tttacttata
     1021 atccctgggc gatcatgaag gtgtcttata accgtgtatt tctgccggaa ggattgccaa
     1081 tcgtctgcta caatcgcgcc tcatttttaa gatggatagc atttttgtat ggtttccgaa
     1141 actaaaacca cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
     1201 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
     1261 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
     1321 ctgatgacca gtctcggtga actggctgca tatatgccgg tttccggttc gtttgccact
     1381 tacggtcaga actatgttga agagggcttt ggcttcgcgc tgggctggaa ctactggtac
     1441 aactgggcgg tgactattgc cgttgacctg gttgcagctc agctggtcat gagctggtgg
     1501 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgctg
     1561 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
     1621 acgacagtta ttgtctttat catcgttggc gtgctgatga ttatcggtat cttcaaaggc
     1681 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
     1741 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
     1801 ggtattgctg caggcgagtc cgaagatccg gggaaaaaca ttccacgcgc ggtacgtcag
     1861 gtgttctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
     1921 ccgtacaccg atccaagcct gctacgtaac gatgttaaag acatcagcgt tagtccgttc
     1981 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
     2041 ctgacggcgg tgctgtcagc gggtaactcc ggtatgtatg cgtctactcg tatgctgtac
     2101 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aactgtcgcg tggtggcgtg
     2161 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtc tgtgcttcct gacctccatg
     2221 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac cggttttatc
     2281 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
     2341 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactgggtcc gatcttcgca
     2401 ttcattctgt gtctgattat cactttgggc cagaactacg aagcattcct gaaagatacc
     2461 attgactggg gcggtgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
     2521 ttcggctaca agctgattaa aggaacccac ttcgtacgct acagcgaaat gaagttcccg
     2581 cagaacgata agaaataagt ttctcccttc cttgctaagc cctctcaacc gagagggctt
     2641 tttcaattcc atttccctga caaatcatgc ggatataaaa tttaacattt ggattgataa
     2701 ttgttatcgt ttgcattatc gttacgccgc aatcaaaaaa ggctgacaaa tcagaggctg
     2761 gtccggtttt ctgggatgat cacctgcata caaataagtc caccgcgatg ctgccgtacg
     2821 caaggggacg tgaagaagat gtgagcgata acccatttta ttttcgtagt tacctcatgg
     2881 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
     2941 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
     3001 tcttccgttg aacaaaacct taaagatgca cccgccagta tcagcgtcat tacccaggaa
     3061 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
     3121 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
     3181 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
     3241 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggtccgt
     3301 ggcccgatgt cgtcgctgta cggttccgat gcgctcggcg gtgtagtgaa tatcatcacc
     3361 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
     3421 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
     3481 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
     3541 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
     3601 aatgtcgaat ttgcctggac accgaatcaa aatcacgatt ttactgccgg atacggtttc
     3661 gaccgtcagg atcgtgattc cgactcgctg gacaaaaacc gcctggaacg ccagaactac
     3721 tccgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaata ctacggtgag
     3781 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
     3841 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
     3901 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
     3961 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
     4021 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
     4081 gcctacctgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
     4141 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
     4201 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
     4261 gggctgtact acatgggtga agaaggctgg ctggaagggg ttgaatccag cgttaccgtt
     4321 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caatgctgca
     4381 ccgggctacc aaaactttgt tggttttaca gcgaacggtg tgccggtatt tagctactac
     4441 aacgttaaca aagcacgtat tcagggcgtg gaaaccgagc tgaaaattcc gttcaacgat
     4501 gaatggaaac tgtcactcaa ctacacctac aacgatggtc gtgatgtcag caacggcgaa
     4561 aacaaaccgc tatccgatct gccgttccat accgccaacg gtacgctgga ctggaaaccg
     4621 ctggcgctgg aagactggtc attctatgtt tctggtcact ataccgggca gaaacgagcc
     4681 gacagcgcga cggctaaaac accgggcggt tataccatct ggaataccgg cgcggcctgg
     4741 caggtgacta aagacgtcaa actgcgcgca ggcgtgctga accttggcga caaggatctc
     4801 agtcgtgacg actacagcta taacgaagac ggacgtcgtt actttatggc agtggattat
     4861 cgcttctgat gagaagatgc ccggcgaacc gggcggactt tcacttcagt aaatactgcg
     4921 catggaagcg caggtgatcc tctataaaag aggcgatgaa gtagtaactg tgatcatatc
     4981 ccggctgaat acgcagcgtc atcggccacg ctttctggcg cgcggcttct gccagaactg
     5041 cgggttgcaa ctgatcggca agaaactgat cattatcgcc ctgatcgata agcgtcggga
     5101 tcgcatcctg cgcgttactg gcatacatca gcgcgcaact gtcccattcc agccatgcat
     5161 ttttgtcctc acctaaatag cggctaaacg ctttgattcc ccacgggacg ctgcacggat
     5221 tcacaattgg cgcaaaggcg gaaacgctgg tgtatttgcc cgggtttttc agcgccataa
     5281 tcagcgcacc gtgaccaccc attgaatgac cgctaatagc gcagcggtcg ctgacattaa
     5341 attgcgactg aaccagcgcc ggtaattcat cgcgcagata atcatacatc cggtaatgcg
     5401 tcgcccacgg cggttgcgtg gcattaagat aaaagcctgc gccctggcct aaatcgtagc
     5461 catcgtcgtt ggcaaccttt tcgccgcgcg ggctggtgtc tggcatcacc agtacaatcc
     5521 ccagttctgc cgccacgcgc tgggcacccg ccttggtggt gaagttctcg tcattgcagg
     5581 ttaatccgga aagccagtac agcactggtg gcggggtgtg atcacgaggg ggggggagaa
     5641 agatactgaa cgtcatcggg cagtttaagg tactggagtc gtgtcgccag cgttgctgcc
     5701 agccttcaaa acagcggtgc tcttcgagca tttccatgcg tggctccttg ttgtgttgtt
     5761 tgcaatttct tcatcatacc gtttattcaa tgtgctgtga gtaactttca cttccgtatt
     5821 tgcataacga tgttttaaca tctgctgatg aaaggcagcg gcaattacaa taattatcgc
     5881 tgtgaatact ggattatgtg cgccgcctca cgcacaataa tcaggctgta aatcagctta
     5941 ataactttgc ccccacgcag ggcggaggcg tcacacctgc aggagaaatc ataaatgcca
     6001 tcactcagta aagaagcggc cctggttcat gaagcgttag ttgcgcgagg actggaaaca
     6061 ccgctgcgcc cgcccgtgca tgaaatggat aacgaaacgc gcaaaagcct tattgctggt
     6121 catatgaccg aaatcatgca gctgctgaat ctcgacctgg ctgatgacag tttgatggaa
     6181 acgccgcatc gcatcgctaa aatgtatgtc gatgaaattt tctccggtct ggattacgcc
     6241 aacttcccga aaatcaccct cattgaaaac aaaatgaagg tcgatgaaat ggtcaccgtg
     6301 cgcgatatca ctctgaccag tacctgtgaa caccattttg ttaccatcga tggcaaagcg
     6361 acggtggctt atatcccgaa agattcggtg atcggtctgt caaaaattaa ccgtatcgtg
     6421 cagttctttg cccagcgtcc gcaggtgcag gaacgtctga cgcagcaaat tcttattgcg
     6481 ctacaaacgc tgctgggcac caataacgtg gctgtctcta tcgacgcggt gcattactgc
     6541 gtgaaggcgc gtggcatccg cgatgcaacc agtgccacga caacgacctc tcttggtgga
     6601 ttgttcaaat ccagtcagaa tacgcgccac gagtttctgc gcgctgtgcg tcatcacaac
     6661 tgattaaaag gcaggaacca tggagcgcaa cgtcacgctc gattttgttc gcggcgtcgc
     6721 cattctgggg atcctgctat taaacatcag cgcctttggg ctaccaaagg cggcttatct
     6781 taatcccgcc tggtacggcg ctattacgcc gcaggatgca tggacctggg catttctcga
     6841 tctcatcggc caggtgaaat tcctcacgct ttttgcgctg ctgtttggtg ctggcctgca
     6901 aatgttgctg ccccgtggca gacgctggat ccagtcgcgg ttaacgctgt tagtcttgct
     6961 gggctttatt cacggtttat tgttctggga cggcgatatt ctgctggctt acgggctggt
     7021 gggcttaatc tgctggcggc tggtgcgcga tgcgccatcg gtaaaaagcc tgtttaatac
     7081 cggcgtcatg ctttatctgg tggggcttgg cgttttgctg ttattggggc tgatttccga
     7141 tacccagacc agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg
     7201 gaagcttcac ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt
     7261 actggcactg ggcgcacagt atggctggca actggctggg atgatgctca ttggtgccgc
     7321 attgatgcgc agtggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg
     7381 ttttgtgctg gtggcgattg gggtgatcat taaccttcct gccatcgccc tgcaatggcg
     7441 gctggactgg gcatatcgct ggtgcgcctt cttacttcag atgccgcggg aactgagtgc
     7501 accgtttcag gcgattggct atgcgtcgct gttttatggt ttctggccgc aattgagccg
     7561 ctttaagctg gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt
     7621 gcaaacgctg atttgtacca cgctttttta tcacctcggt ttgtttacgc agtttgaccg
     7681 tctgggattg ctggcgtttg ttattccggt atggctggcg aatattctct tctctgttat
     7741 ctggctgcgt tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg
     7801 tgctgccgga ccggcaatat ctaaaacatc aagataacga tctggatcac attcgttaac
     7861 aaaacggctg taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc
     7921 tgacaaaata gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat
     7981 gatcaccatt cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt
     8041 gctcaataac agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag
     8101 tgagctggat tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac
     8161 cattggcgtg gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt
     8221 ggatctggtc gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc
     8281 ggaaaaagaa cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cattgattgt
     8341 tcactcaaaa gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat
     8401 ggtgttaatc aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct
     8461 cagcggtgcc cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta
     8521 tctttcttcc agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc
     8581 gttgaaagag caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat
     8641 gccgggcggt gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt
     8701 atttgcttat aacgacaata tggctgctgg cgcactgaca gcattaaaag ataatggcat
     8761 tgcgattccg ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac
     8821 cgacccgcaa ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga
     8881 actggccttg cagggtgcag caggcaatat tgatcctcgt gccagccact gttttatgcc
     8941 gacgttagtg cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc
     9001 aacaaatcag gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat
     9061 gtgatagcta tgattgcacc gttttaacgt tgtaacccgt atgtaacagt gaataatcac
     9121 tttcaccgag gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg
     9181 catggaagaa cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta
     9241 ctgccccgcc gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat
     9301 gaataagaag gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc
     9361 acacgctgct gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc
     9421 tgtagtgcgc aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat
     9481 gaatgattct cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa
     9541 aggggtgaag gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga
     9601 gaaagcgcgt gggcaaaacg tgccggtggt tttctttaac aaagagccgt ctcgtaaggc
     9661 gctggatagc tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat
     9721 tcagggcgat ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaataaaga
     9781 cggtcagatt caattcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg
     9841 taccacttac gtgattaaag agctgaacga caaaggcatt aaaactgaac agttacagtt
     9901 agataccgca atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg
     9961 cccgaacgcc aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc
    10021 ggtagaagca ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgctct
    10081 gccagaagcg ctggcgctgg tgaaatccgg tgcactggcg ggcaccgtac tgaacgatgc
    10141 taacaaccag gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gtaaaggtgc
    10201 ggctgatggc accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt
    10261 agataaagac aacctggctg aattcagcaa gaaataagac tgattgggcg cagtctatta
    10321 ctgcgccctt attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc
    10381 ttccggggaa tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc
    10441 acttgataac gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa
    10501 cggtgcagga aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg
    10561 caccatttta ttccagggta aagagatcga tttccattcg gccaaagaag ccctggaaaa
    10621 tggtatttcg atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa
    10681 catgtggctg gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg
    10741 cgaaaccaaa gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg
    10801 cacattatct gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc
    10861 gaaaattgtg attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct
    10921 gttcactatt attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa
    10981 aatggaagaa atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat
    11041 cgccaccgaa ccgctggcag gactgacgat ggataagatc atcgccatga tggttgggcg
    11101 ttctcttaac cagcgtttcc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt
    11161 gcgtaacctg acgtcactgc gccagccgtc gatccgtgat gtctcgtttg atctgcataa
    11221 aggggagatc ctcggtattg ccggtctggt gggggcgaaa cgtaccgata ttgttgagac
    11281 gttatttggt attcgcgaga aatcggctgg caccattacg ttgcacggca aaaagatcaa
    11341 taaccataat gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg
    11401 ctcaacagga atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg
    11461 caactacaaa aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg
    11521 ggtgattgat tcgatgcggg taaaaacacc ggggcatcgg acgcaaattg gttcgctctc
    11581 cggtggtaat cagcaaaagg tgattattgg gcgctggcta ctaacgcaac cggaaatatt
    11641 aatgctcgat gaaccgacgc gcggtattga tgtcggggcg aagtttgaga tctatcagtt
    11701 aattgccgaa ctggcgaaga aaggcaaagg gattattatt atctcctctg aaatgcctga
    11761 gttgttaggg ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt
    11821 cgatacaaaa acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga
    11881 ttaggggctt cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg
    11941 cggtatttac gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt
    12001 tttaagtctg ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc
    12061 gctcggtgtg gcagggttaa ttgtcaccca ggggaccgac ctttctgcgg ggcgtcaagt
    12121 agggctggcg gcagtggtgg cagcgacatt attgcagtcc atggataacg ccaacaaagt
    12181 gttcccggaa atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg
    12241 tgcggtgatc ggtttgatca acggtctgat tatcgcttat ctcaacgtta cgccgttcat
    12301 taccacgctc ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgatttcgt
    12361 cggggcgtcg ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt
    12421 cgcgctgggg agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt
    12481 cgtctgggtg ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa
    12541 cccggaagcg gcaaaagtat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc
    12601 gttgtctggc gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc
    12661 caccaacaac ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg
    12721 cgtatcgttc agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt
    12781 taccgtcatc aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat
    12841 caaaggggcg attattatct tcgccgtagc gctggattca ctgaagtacg cgcgtaagaa
    12901 gtgatatgat tgtggtccgc attcggtcat aaacgcacat aacatcatga tctgtgtggt
    12961 tttgtaggcc ggataaggcg ttcacgccgc atccggcgac aggagccaaa tgccggatgc
    13021 aacgctggcg cgttttatcc ggcctacaat gatctcaaca tactgatttg tatggttttg
    13081 taggccggat aaggcgttca agccgcatcc ggcgacagga accaaatgcc ggatgcgtct
    13141 ctggttttac aacgttaccg ggtgttcttt ctcgcctttc ttaaacttca cttccccgag
    13201 ctcaatacaa cccaccgggc agacgtgacc acaaagcaga caacccacac atttctcggt
    13261 attacaatgc ggtgtgcggg ttttctcgct ccattccatc gcctggtgac cgccgtcgta
    13321 acaggaaata taacagcgtc cacagccaac acatttatca agattgatac ggggatagac
    13381 aatataactg cggtctaaat cttccgccgg gacaatattg ttattcgcca gacctaccat
    13441 ctcctgcaac gaatcaaatc cctgatcggc gagataatgc gacaacccgc tcgccatatc
    13501 ttccactatc cgatacccgt actgcataat gccggtggtc acctgtaacg ttgctgcgcc
    13561 gagcaataaa aactccgccg catcctccca ggtttcaatg ccgccgatac cgctgattgg
    13621 gaaatcgcgc agttctggat gggtgcgcat ttgctgaatg aagcgcaggg cgatcggttt
    13681 taccgcttta ccggaatatc cggaaatact cgattttccg ttaacgatcg gcataccgat
    13741 tttctgatta agatcgatat tggtgatgga tttaacggtg ttaatcgccg caatgccatc
    13801 tgcgccgccg cgcttcgccg ccagcgccac ttcgcacata tcaccgatat tcggcgtcat
    13861 cttcgccagc attggcagcg tggaaccccg tttcactgcc cgacaatatt tttctaccag
    13921 ctccgggctt tgcccgacat cgctacccat cgcatgagaa gtcatttgcg gacaggagaa
    13981 gttacactcg atcatatccg cgccagcttc ttgcaccagg cgcgccagct cctcccattg
    14041 ctgctcattt tcccccatga tcgaagcgat caatactttg tccgggtaat cttccttcag
    14101 ccgacgcagg gcggccagat tctcttccaa cggatgttca gcaatctgct ccatattttt
    14161 gaagccgata aaaccggtat cttctttcac cagatgatca aaacgcggcg agacttcgtt
    14221 ggcgataaaa aagccgatcg ttttaaacac cacgccgccc caacctgtgt cgtaggcttt
    14281 ggcacacatc tcatagcagt tgcctaccgg cgaagaagag aggcagaacg ggttgggaaa
    14341 cttcacgccg caaaaagtaa tcgaaagatc tttcgttaac atgagcaagc tccctctaaa
    14401 tagtgatgaa tcgcctcggc ggcttctttc ccggttttca cggcatagac cacggttttg
    14461 tcaccctcaa caatatcgcc agcagcaaag acttgcggat cgcgggtctg ataattttgt
    14521 gttttgatgg tgttacgctg cggctctaac tccgcaaagg catccagtct ggcatgctga
    14581 ccgacggcga gaataatttt atctgccacc atcgtcagtt cgcccgataa ccgtacatgc
    14641 ttaaacgtga ctttattgcc ttccacggct actggcgtga atccatcgat gatcgaaacg
    14701 cccagctccc gggcgctggc aaactctttt tcgcttgccg gaaactcatc taactcttca
    14761 cgcgctatgc aggttaccgc ctgacagccg agaactttca gcgtgctggc tacgtccatc
    14821 gcgacatcac cgccgccgat aattaatgcg ctttgtggaa tgctgatatc gccttgcgcc
    14881 tgtcgtgcac gtagcaggaa gtcgacggca atctcaacgt cgctatgctc aaacagcggt
    14941 agcccggaac cgcttgataa ccccacggtg accagtaccg cgcggttttc cgctttcagt
    15001 tgctccaggg tgagtgtttt accgatttcg ttattgcact taatggtcac gcccattttt
    15061 tcaatacggg cgatctctgc atccagcact gattgcggta accggaattg cggaataccg
    15121 ttacgcaacc agccaccggg gtgcgcttct ttctcataaa tcgtgacgtc ataaccctgg
    15181 tttgtcagtg tcacactggc ctgcaatccg gcaggacctg cgccaataat cgcgactttg
    15241 ccgagcgttt tagtaccagg ctgataaatt tccatccctg tttgttgttc aaaatcagta
    15301 acaaaacgct gtaagcggcc gatatcaatg ggagcatcaa caccggcacg ggtacaaccg
    15361 ctttggcata atttctccgt cgggcacact ctggcacaaa cggcaccgag ggcattattt
    15421 tcgcgaattg tctcggcagc gcctttaaaa ttacgaaagt agattgagcg aataaatttc
    15481 cccggatcgg tctgtgccgg gcaagcctga ctacagggag cgtcgtgaca taataaacag
    15541 cgagaggctt ctttaatcgc cagtaaaggc gtaaaagccg gagtgagttc atccagataa
    15601 ttttgttgcg gcatgtgatc ttccttaatg ctatgagtaa ttaaggcgaa cgctaaataa
    15661 atattgcgtg ctgatattaa ggctatgcgc gtggggaaat ttaaactgtg aaaggattca
    15721 caaatagctg cggttcagaa tgttatatcc gcagcttatt gagtgaagta ataattattc
    15781 tcaataatta acagaaaaga gaaaatatta acgatagaaa ataagataaa caatcatgcc
    15841 gatagataaa attccgaagc cgatatatgc caccatataa gcgaaaaaga gtccgggatt
    15901 ttttgctcgc cagacataca ccggttccgg tgcattttta acccgtttat caatcgcgcc
    15961 tgcgcgccag cctttccatg cttcgcggaa acaaaatatc gggataagga aaaaaacggc
    16021 aacgacaaaa aactgctgta catccataac tactttcctt gtttcttttg taattcaagt
    16081 aactgttcgg gtgtgacggc gggatacccc tgcgcatctt ccggcacctg gtgcatagcc
    16141 ggaataggga ccagcggccc taaaaaacgc ggttcacgtt taaaaatata aaggtcagcc
    16201 agcgcaccga aacgggcggc aaattcgcga atacgtactg acagcatatc tttcggtgac
    16261 ggtacggcat aacactgagc ctgaatcccc atatgcagcg caataaataa tgcccgctca
    16321 cagtggaaac gttgggtgat aataatgaaa tcattagtat cgaaaacttt gcgtgtacgc
    16381 acgatggaat ccagcgtacg aaagcctgcg taatcgagaa caatatctga tgggtcgaca
    16441 ccagcagcga ttaaatcttt gcgcatggtc atcggctcat tataactttg caatgcgtta
    16501 tcgccgctca gtaatagata atttacctta ccgctgttat aggcattaat cgctccttga
    16561 atgcggtagc gataatactg attaattacg ccagtacgat aatattttgc tgttccgagc
    16621 accacaccga cctggcggta ggggagatcc tgcaattcgt cgtagatata aggcgcggtt
    16681 ttccagctca tccagcgatc gaggccgagc acagtcaaca gcagcaagcc gataaggact
    16741 aacaggctga ggaacacgcg ctttaacatg caggagactc gatctaagga aaatttccat
    16801 caggctactt tacccgctgg ttttgcgcaa gaaacggcaa actgtttgcc gggattttcg
    16861 acaccaggag agggctggca agccggacaa ggcgttcacg ccgcatccgg caccaggctt
    16921 aagcgagaag cacgcggtcg atactgtgac agccgagcgc tttcagcgtt gcggaggtgg
    16981 catcccactg aatcaacggc gcatcggctt tttctgccag aaccgcgcgc tggatatccg
    17041 ggtaatcata acccttgaga ttcaacagaa ttaacgctcc ctgcaacggc ggcagagtcg
    17101 ggttgaatgc ggcgttttca gcgtagctgc cgctgaaaat tcgaccgtct ttacattcca
    17161 gcgcaacacc gcttggcgac ttactgtaag gcatgtgcga acggtttgcc gccgcaatcg
    17221 ccgcctgaga aagcgcatca ccagtcagcg catagccgtg atcctgctcg tccatcagca
    17281 gcgttttaat ctccagatct ttcggcccaa aggcatccgg cagatagtca cgcagcgcgt
    17341 gtgcctcgcg gcccggcaga tgaatacgca gatccagacc gctgttcagt tcattcataa
    17401 actgacggca gtgaccacaa ggcgtgtagt taacggtgat ggctgcaagc gctttttcac
    17461 cactcaacca ggcgtggctg atcgcgcttt gttcggcatg aacggtttgc tgcattgtcg
    17521 caccaataaa ttccatattg gcaccaaaat accaggttcc gctcacaccg cgcgcaattg
    17581 cgccaacatt aaaattcgac aatggcgtac gcgcacaggc cgccgccagc ggaagtagtg
    17641 cgaatgccag cgcgtcttcg tccagccccg ttgcgctctt cagcgatgag acttgctccc
    17701 cggtcaacaa agcggggaag tacttgtctg ccagaatagg ttccagtgca gattgcaagt
    17761 tttccgcaag ttgggcaaaa gcggtttgaa aacgtggatg catggcgttg cctcataatc
    17821 atgtaatggg ataacagttt acgaacccgt tatggcttta tatgtgatct gaatctcatt
    17881 aattatgcaa cgcgtgaaac ttacatcaaa aatgcgcgac gcatcgcaaa ttttagccca
    17941 ttactgccag aatcatcggg aaaaggaacg gtgcgatcag cgaagtaatt atcccgcata
    18001 acaccagcgc tagcgaacta aatgcacctt cctgataatc cagctcggcg cagcgcgctg
    18061 taccgagggc gtgcgaggca gtccccattg ccagaccgcg cgcagctttg gtacgaatac
    18121 gcatcgcatt aagcaatgta tggccaaata ccgcgccgag gatgccgacg aaaatcacgc
    18181 aaactgcgct gattgccgga ataccgccaa tactgccgcc aaccgccatt gcaataggcg
    18241 tggtgactga tttcggcagg attgacgcgg cgatttccgg tgaagcgccc atcaacaatg
    18301 ccacggaagt ccccgtcacc atcgcaacca cgctgccgat aaaacagatg gtgatgatcg
    18361 atttccagcg cgcgcggatc tggtgtagct gctcatataa aggataggcc agcgcgacga
    18421 ccgccggttg cagcagatcg tttagcactt cgctaccttt aaagtaactg tcgtaagaga
    18481 tgccggtcag cattaaaaaa ggaatgatca ccaccattgc taccagcaac gggttaagca
    18541 ggggaaactt ataccgtgcc gccagtttgc gggcggcaaa aaagacaatc aacgtcagcg
    18601 gtaatgacca ccagatattc gccatcattc ttctgatcct ttctgaccta cgactttacg
    18661 ttcaccgtgt accagttgcg aactccagct caccaccaga aaaaccacca gcgtactgat
    18721 tgcacaggaa accactaccg ggccaaactg tgcgcggagc aaatcaaaat attgcatgac
    18781 gcctacgcca atcggcacaa acaatagcgc catatagcga atcagtacgt agcaccccgg
    18841 attgacccat ttcgccggaa gaatttgcaa ggccagcagg acaaacagga tcagcatccc
    18901 gatgatgctg cccggaatgg ttaccggtag cagggaagca atgaaaatgc ctgcatacag
    18961 gcaggcataa atcaggacga aagcgcgtaa atattgccag ataatgttca gtgtcttact
    19021 cataatcaac accccagatg agatggattc atcatacaat taattcgtaa aacgtgctac
    19081 cgatcacatt aagaattctc agcatagttg aattttaggg aggtaacctc gggcgagcgc
    19141 cccgttcagc acactaattc tgctgtaaaa agtgcagaaa ctgacttaaa gcataagaag
    19201 gcgtttcgtt attgcgccag aacactccga cgctaccttt ctgttctatc tttggcaggt
    19261 tgagtatcac cagttgtccc tggctggcat aacgttgcgc caggcgtaat gacaaaatag
    19321 agatcatatc gctgctttgt aacaggttgg tactgacgtt catcgacgcc gactcaatcg
    19381 tgttttctgg cagcattacg ccgtggtcta ccagcgcatt atcaatacta atgcgaatgg
    19441 gggtacccgt cggccagacg atccagcgcc agttggcaag atcattccag ctgagggtgg
    19501 cgtatttcgc cagtgggtgc tcagggcggg cgacaaagca tactggctcg gtgtacagca
    19561 cctgataatt cagtggcagc tgcaatgcgc gaccgccaat tcgcccaacg accacatcga
    19621 cagaacctgc caatagatct tgcagcaatg gggtcatcac cttctcttcg atattcaggt
    19681 gtagagttgg catcttcgta agcaaatgca aaatcgcctg ggatacgcag tcagtggcga
    19741 cgggagaaca gccaattttc agactgccga ccagtccacc ttgtttaaaa cgggccagtt
    19801 catgttgcga tcgctccatg tcattgatta accgttgggc atgttgcaat aacaatttgc
    19861 cgccttcagt cggacgtaat cctttgctgt gccgctcaaa aagtgtgatt ccaatctcat
    19921 cttcgagttg tgacaaccat ttcgaaagcg caggttgggt gatattcatc attcgtgcga
    19981 catgggttag attgccctgt tcacccagcg ccaccagcat ttcaaggtga tgtagtttta
    20041 atttttgcgc ccagttcgcc atttgccata acctccaggt tatctctctg cccgaaatgc
    20101 cattgtacat ctcatctctt taatcacaat atcgcaacat atacaaaaca aaagaaacat
    20161 ctaccttacc tgcactgagg catttactat gactcaacga cgtgatttac aagcacttat
    20221 cgatgctgct ccggtcggca agatgcagtg gcgcgttatc atctgctgtt ttctggtggt
    20281 aatgctcgat ggttttgaca ccgcggctat cggatttatc gcccctgata ttcgcactca
    20341 ctggcaactc accgcaggcg atctcgcccc gctttttggc gcgggtctgc taggattaac
    20401 cgcaggcgca ctgctttgtg gcccgttgtc cgatcgcttt ggtcgcaagc gggtgattga
    20461 gctgtgcgtc tttttgtttg gcgcgttaag cctcgcctcg gctttctcac cggatctgca
    20521 aacgctggtc ttcctgcgct ttctcaccgg cctgggtctg ggcggcgcga tgccgaacac
    20581 catcaccatg acctcggagt atctgcctgc ccgccgccgc ggcgcgctgg tgacgctgat
    20641 gttttgcggc tttacgctgg gttcggcgtt tggcgggatt gtcagtgcgc agctggtgcc
    20701 ggtgattggc tggcacggca ttctggtgtt ggggggcgtg ttaccgctga tgctgtttgt
    20761 cgcactgctg gtagttctgc cggaatcgcc acgctggcag gtgcgccgcc agctaccgca
    20821 ggcggtgatt gcgaaaaccg tcagcgccat cacccgcgag cgttatgtcg atacccactt
    20881 ttatctcatt gaatcagcct ccgttacaaa aggcagcatt cgtcaactgt ttatgggacg
    20941 tcagttaccc atcacgttga tgctgtgggt agtgttcttt atgagcctgc tgattattta
    21001 cctgctctca agctggatgc cgacgctact gaaccatcgc ggcatcgacc tgcaacacgc
    21061 gtcctgggtc accgccgcgt tccagattgg tggtacgtta ggcgcgctgg cgctgggtgt
    21121 gctgatggat aagttcaatc ctttccgcgt gctggcgctg agctatgcca taggcgctat
    21181 atgcatcgtg atgattggct taagccagga tggattatgg ttgatggcgc tggcgatttt
    21241 cggcactggc attggcatta gcggatcgca ggtggggctg aatgcgctga ccgcaacact
    21301 ttatccaaca caaagtcgcg ccacgggcgt gagctggtca aatgctatcg gccgctgcgg
    21361 cgcgattgtt ggttcgctgt ctggcggcgt gatgatggcg atgaatttct ctttcgacac
    21421 cctgtttttc attatcgctg tgcctgcggc gatcagtgcg gtgatgttaa ccctgctgat
    21481 taccgtggtt cgccagtcga cctctgtccc tgactcactg cctcgcgcag gcgttgtgaa
    21541 cgaataagga gcatcccaat gactgataac aatcaaaata gccgcgagca attttatcag
    21601 catatttccg ggcaaaacct gaccccgctg tgggagtcac tgcaccatct ggtgccgaaa
    21661 acgcccaacg ctaactgtgc gccagcgtac tggaattatc aggagatccg cccgctgcta
    21721 ctggagagcg gcggcctgat tggtgcgaaa gaggccgtgc gccgcgtgct ggtgctggaa
    21781 aacccggcgc tgcgcgggca gtcttccatt accgcgactt tatatgccgg tttgcaactg
    21841 atcatgccgg gcgaagtggc accgagtcat cgtcataatc agtcggcgct gcgttttatt
    21901 gttgaaggca aaggggcatt taccgccgtt gatggcgaac gcacgccaat gaatgaaggc
    21961 gattttatcc tgaccccgca gtggcgctgg cacgatcacg gtaaccctgg cgacgaaccg
    22021 gttatctggc tcgatgggct ggatctgcca ttagtgaata ttctgggctg cggttttgcc
    22081 gaagattatc cggaagagca acaaccggta acgcgtaaag agggagatta tctgccgcgt
    22141 tacgctgcca atatgttgcc gctgcggcat cagacgggga actcctcgcc gatctttaac
    22201 tatcgttatg accgcagccg cgaagtgctg cacgatttaa cccgactggg cgatgccgac
    22261 gagtgggatg gctacaaaat gcgctacgtc aacccggtca ccggcggcta tccgatgccg
    22321 tcgatgggcg ctttcctgca actgctgccg aaagggtttg cctcacgcgt tgcgcgtacc
    22381 actgacagca ccatctacca tgtggtggaa ggtagcgggc aggtcatcat cggcaatgaa
    22441 accttcagct ttagtgcaaa agatatcttc gtggtgccga cctggcatgg cgtgtcgttc
    22501 cagaccacgc aagattccgt tttatttagt ttttcggaca gaccggtaca agaagccctg
    22561 gggctgttcc gcgaagcgcg ttattaattt aggagagaaa aaatgactca atacgtattc
    22621 gaaccgcaag cgcccgttac cgtaccggtt gttggcagtg atgaacaatt ccccgtgcgc
    22681 cgcgtttact gcgtcggacg taactatgcc gctcacgccc gcgaaatggg ctttgatccg
    22741 gatcgtgaac cgccattctt cttctgcaaa ccggcggatg ccgttgttcc ggttgccgct
    22801 ggtgaaacgc tggcgctgcc gtatccggca cagaccgata actaccatta cgaaattgaa
    22861 ctggtggtgg cgattggtaa aaaaggcagc gatatcccgc tggaacacgc ccatgaatat
    22921 gtctggggtt atgccacggg tcttgatatg actcggcgtg accgtcaaat ggaaatgcgc
    22981 cagatgggcc gcccgtggga aatcggtaaa gcgtttgatc tctcagcgcc catcgcgccg
    23041 ctgcacaaag cccgcgatat cggcggtatt gataatgcgc caatctggtt acaggttaac
    23101 ggcgaagatc atcagcgcag cgatattcgc catctcatct ggtcggtgaa tgaaaccatc
    23161 agctatctgt caggcttctt tgagctgcaa ccgggcgatc tgatcttcac cggtacgccg
    23221 gaaggggttg gcccggtagt gaaaggtgat gtgatcacag gcaacgtcga gggcttaacg
    23281 cctatcgcgg tgaagattgt ctgaggtgca tgatgaagct gtacagtttt tttaatagtt
    23341 cagcgtcgta ccgggtacgc attgcgctgg cgctgaaagg cattaattac cagaccgaag
    23401 gggtgaatat ccgtattggt cagcaaaatg aactggccta tcggcggatg aatccggtcg
    23461 ggctggtgcc gacgctgctg actgacgagg gcgaatcact cggacaatcg ctggcgatta
    23521 ttgactggct ggagaggcac tacccgcaag ttccgctggt gccgcaggaa gagccggccc
    23581 gcaacaaggt gctggaaatt gtctatgcca tcgcttgtga tattcatcca ttgaataact
    23641 tgcgcgtgct gcgctacctg accgaagaac tgaacgtcag tgaagaagaa aaaaaacgtt
    23701 ggtatgcgca ctggatccag caagggttaa gcgcggtgga acaactgttg cgccagaacc
    23761 agtcggggca attttgtgtg ggcgagacgc cgacgctggc agattgctgc cttgtcccgc
    23821 agtgggcgaa cgcgctacgc atgaactgcg atttgagcgg gtatccacgc tgcaaagcgg
    23881 tgtacgacgc ctgcacacag ctgccagcgt tcattgccgc agcgccagaa aatcaacaag
    23941 ataaaatttc agcctgacca ggagaactat aatgacgaaa gtgacacgcg cagtaattgt
    24001 gggaggcggg atcggcggtg cggcaactgc gctgtcactg gcccgcctgg ggatcaaagt
    24061 ggtgctgctg gaaaaggcgc atgagatcgg tgagattggc gcgggcatcc agttggggcc
    24121 aaatgcgttt tcggcgctgg acagcctcgg cgtcggcgaa gttgcccgcc agcgcgcggt
    24181 gtttaccgat cacattacca tgatggatgc ggtgaacgcc gaagaagtgg tgtgcattga
    24241 aaccgggcag gcttttcgtg accatttcgg cggcccttac gcggtgatcc accgggttga
    24301 tattcacgcc tcggtgtggg aagcggtact gacgcatccg gacgtggaat atcacacctc
    24361 caccaacgtc gtggatattc gcgagacgga agacgatgtc accgtgtttg acgatcgcgg
    24421 caatagctgg accggcgata ttctgattgg ctgtgacggc gtgaagtcag tggtgcgcca
    24481 gagcttattg ggcgatgcgc cgcgtgtcac cggacatgtt gtctaccgtg cggttatcga
    24541 tcgtgccgat atgccggaag atttgcgcat taacgcaccg gtactgtggg cggggccgca
    24601 ttgtcacttg gtgcattacc ctttgcgcgg cggcaagcag tataacctgg tggtaacatt
    24661 ccacagccgc cagcaggaag agtggggcgt gaaggatggt agtaaagagg aagtgctctc
    24721 ttactttgaa gggattcacc cccgtccgcg ccagatgttg gataaaccga cttcatggcg
    24781 acgctggtca accgctgacc gtgaaccggt ggcgaagtgg ggaacgaagc gcataacgct
    24841 tgtgggcgat tcggcccatc cggtggcgca atatatggcg cagggagcct gtatggcgct
    24901 ggaagatgca gtaacgctgg gtaaggcgct ggcgcagtgt gagggtgatg cggcgcaggc
    24961 ctttgcgctg tatgagtcgg tacgaattcc gcgcaccgcg cgcatcgtct ggtcgacccg
    25021 tgaaatgggg cgggtttacc acgccgcagg ggtagaacgc caggtacgta atctgctgtg
    25081 gaaagggaaa tcgcaggcag aattttatcg cggcatggag tggttgtacg gctggaaaga
    25141 agataactgt ttgcaaccac gctgagcact tcttcaccca atcaggcgca cgggcggtcc
    25201 cctcgcccct tcgggagagg gttagggtga gggtaaaaat cttgcccgaa ttaatcagta
    25261 agtcagtttg tctgcaaaca caggaggcgc taacatagcg cctcattttt ttgcgggtga
    25321 tgatatgcgt gtgttactgg caccgatgga gggtgtgctt gactctctgg tgcgtgaatt
    25381 gctgaccgaa gttaacgact acgatctgtg catcaccgag tttgtccgcg tggtggatca
    25441 actgctgccg gtaaaagtct ttcatcgcat ttgccctgag ttacaaaacg ccagccggac
    25501 accatccggt acgctggtgc gcgttcagtt gttaggtcag ttcccacaat ggctggcaga
    25561 gaacgccgcc cgtgcggtgg aattaggttc ctggggcgtg gatctcaatt gcggctgccc
    25621 gtcgaaaacg gtcaacggta gcggtggtgg ggcgacgtta ctcaaagatc ctgaactcat
    25681 ctaccagggt gcaaaagcga tgcgtgaagc tgtaccggcg catttgcccg tcagcgtgaa
    25741 agtgcgtctg ggctgggaca gcggtgagaa gaaatttgaa atcgccgatg cggttcaaca
    25801 ggctggtgct acggagctgg tggtgcatgg acggacaaaa gagcagggtt accgcgcgga
    25861 gcatattgac tggcaggcga ttggtgagat tcgccagcgg ctgaatattc cggtgattgc
    25921 caacggtgaa atctgggact ggcagagcgc gcaacaatgc atggcgatca gcggctgcga
    25981 cgcagtgatg attggtcgcg gggcgctcaa tattcccaac ctgagccggg tggtaaaata
    26041 taacgaaccg cgaatgccgt ggccggaggt ggttgctttg ctgcaaaaat atacccgtct
    26101 ggaaaagcag ggcgataccg ggttatatca cgtagcgcgg attaaacagt ggttgagtta
    26161 tttgcgtaaa gaatacgatg aagcaacgga attatttcag catgttcggg tgttgaataa
    26221 ttcccatgat attgcaaggg ctattcaggc aattgatatc gataagctgc gataatagtg
    26281 gaatcgcagt ttatcagaga caatgaatcg cttattcagc ttcatcatct tgttgcgctc
    26341 ctttcatgaa ctaagccaca tattgccact ggcgcaagga gcgcgcaggg ggagggcaat
    26401 cgcctcctcc ccctgcaccc ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt
    26461 cagcttatcc ccttcggcta ccgggtcggg cgcgaggaaa cgtccttgta agacgcgccc
    26521 tcagcccaca tccatgtggg ctgccccggc cttcggggaa cgcttcagcg attttgacgc
    26581 caccaacacc ggagctgttt gcaaatcaag tacacaaaat atctagttcc tgacctgaca
    26641 acttatatgt catatctgat ttttaaaata tcatcttaaa tacgccagtc accaccagta
    26701 gcccaatcag gaaaataatc aatacagccc agagtataat tttcattggc gttcctttat
    26761 ttgtgagtgc tgaataagta taggaaaaaa cgaagccctt tgccgtt
//
LOCUS       DAZKFU020000040        26523 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZKFU020000040 DAZKFU020000000
VERSION     DAZKFU020000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.040"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1270
                     /locus_tag="ACHOQQ_RS06760"
                     /old_locus_tag="pgaptmp_001352"
     CDS             <1..1270
                     /locus_tag="ACHOQQ_RS06760"
                     /old_locus_tag="pgaptmp_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HGU4469625.1"
                     /translation="APLPQWANAPAVTPVAQLSLQESILRAFARNPDVTQQAAQIGIG
                     EAQIDEAKSAWYPHVGLTGNAGPSRQTDSSGRLDNNVSYGITLTQLVYDFGKTNNDIN
                     LQTAARDSYRFKLMATLTDVAEKTATAYMEVSRYQALCDAAQRNIHSLENVYNMAALR
                     ANAGLNSSSDELQAQTRIAGMRSTLEQYQAQMVSAKAQLAVLTGVQPEAIAAPPAELA
                     EQPVSLKNIDYQSIPLVLAAENLRQSAQYGVEKTKAQYWPTLSIQGGKTRYQTSDRSY
                     WDDQLQLNVNAPLYQGGAVSAQVQQAEGQQKISASQVEQAKLDVLQRASVAYANWTGA
                     RGREEAGLAQSESAHKTRDVYQNEYKLGKRSLNDLLTVEQDVFQAQSAEINANYDGWV
                     AAVNYAAAVNNLIPLAGIKQGLYNDLPDLK"
     gene            1373..22150
                     /locus_tag="ACHOQQ_RS06765"
                     /old_locus_tag="pgaptmp_001353"
     CDS             1373..22150
                     /locus_tag="ACHOQQ_RS06765"
                     /old_locus_tag="pgaptmp_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU4469626.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTTVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDTTGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGEVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINVAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVDAL
                     ADGNATVTASVNDVAGNPTSVSRVALVDATPPVVTINPVATDNVINTPEHIQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSSIGNSTTASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAAVGDAVTVTLGGNNYTTTVQAGLSWSVD
                     VPADAIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSLGLAEGTPLTVTINDVEYTTAVQADGSWSVGITAAQVSAWPAGAVNI
                     AVSGESSAGNPISITHPVTVDLTPAAITINTIATDDVINAAEKGANLTLSGTTTNVEA
                     GQTVTVTFGGKNYTASVAGDGSWTATVPAADLAALPEGSASAQASVSNINGNSASAVH
                     SYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTVTLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDSETHTVTVNLTAPTIGINTIATDDVINATEKGAD
                     LQISGTSNQPAGTTITLTLNGQNYTATTDASGNWSTTVPASAVGALGEASYTVTANVT
                     DSAGNSNSASHNVQVNTALPGVTLNPVASDDIINAAESGVAQTISGQVTGAAAGDTVT
                     VTLGGKTYTATVQGNLSWSVDVPAADIQAIGNGELTVNAWVTNGVGNTGSGSRDITID
                     ANLPGLRVDTVAGDDVVNSIEHGQALVITGSSSGLAAGAALTVVINNVTYGATVLADG
                     TWSLGVPAANVGNWPAGTVNITVSGATSAGNPVTITHPVTVDLAAVAISINTVSGDDV
                     INAAEKGADLTLSGSTSGVEPGQTVTVTFDGKTYTATVAGDGSWTTTVPAADLAALRD
                     GDATVQASVSTINGNTASATHVYSVDATTPTLTINTIATDDILNAAEAGNPLTISGSS
                     TAEAGQTVTVTLNGVTYTGTVQADGSWSVSVPTADLSNLTASQYTVSASVNDKAGNPV
                     SATHGLAVDLTVPVLTINTVSGDDIINATEHGQALVISGSSTGGEAGDVITVTLNSKT
                     YTTTLDASGNWSVGVPLSDVTALGSGPQNITATITDAAGNSDDASRTVTVNLAAPTIG
                     INTIATDDVINATEKGADLQITGTSNQPAGTTITVTLNGQNYTATTDASGNWSATVPA
                     SAVSALGEANYTVTANVTDKAGNSNSASHNVLVNSALPAVTINAVATDDIINADESDN
                     AQTISGQVTGAAAGDTVTVTLGGNTYTATVQANLSWSISVPAADIQALGNGDLTINAL
                     VTNGVGNTGSGSRDITIDANLPGLRVDTVAGDDVINSIEHNQALVITGSSTGLTAGTA
                     LTVEINNVTYAATVLADGTWSLGVPAVDVSNWPAGTVNITVSGTNSAGTTSTITHPVT
                     VDLAAVAITINTLSGDDVINAVEKGETLVVSGSTSGVEAGQTVTVTFGGKNYTTTVEA
                     NGSWTVNVPPADLAALPDGAGNVQASVSNINGNNAQADRAYSVDATAPLVTINTIASD
                     DTLNVSEAGAGITISGTTTAQAGQTLTVTLNNNTYQTTVQADGTWSVNVPAADLSGLT
                     ASSYTVTATVSDKAGNPASADHALAVDVTAPDLTINTVAGDDIINAIEHGQALVVSGT
                     STGAAAGDVVTVNLNGKNYTTTLDASGNWSVGIPAADVTALATGSQTITASLSDRAGN
                     SDSTTHNVTVDLSGPTLTISIVSGDDIINNAEKTQDLIISGGSSGLATGTTVTVMLNG
                     LAYSATTDGSGNWSVTVPASAVGALGEAVYQISASATDSAGNSGITTHTVNVESLLPG
                     VIINTVAGDDIINAAEIGVNQTISGQVTGTAAAGDTVTVTLGGNQYVATVQPDLSWSV
                     SVPANVLQALGNGELTISASVTNSANNTGTTTHDIVIDANLPGLRVDTVAGDDVINSI
                     EHTQALVITGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPAADVTNWPAGTVN
                     IAVSGTNTAGTTTSITHPVTVDLAAVAITINPLSTDDVINAAEKGSDLQLSGTTSDVE
                     AGQTITVIFGGKSYTTTVAADNTWGLTIPAADLAALPDGAANVQASVSSVAGNSAQAT
                     HVYSVDATAPSVTINTIAADDILNAAEAGSALIISGTSTTEAGQTVTVTLNGVNYSGN
                     VQADGSWSVSVPTGDLANLTASSYTVNASVSDKAGNPASATHNLTVDLAAPVVTINTV
                     AGDDIINATEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVI
                     SALAQGDVNISATVTDSAGNSGTASHVVSVALGAPVLAINTIAVDDIINATEKGADLA
                     ISGTSDQPAGTQITVTLNGQNYTTTADASGNWSVTVPASAVGTLGEATYTVTASATDA
                     DGNSGSASHNVQVNTALPGVTINVVATDDIINAAEAGVDQTISGQVTGAAAGDTVTVT
                     LGGATYTATVQANLSWSVDVPASALQALGNGELTISASVTNSVGNTGNGTREITIDAN
                     LPGLRVDTVAGDDVVNIIEHGQALVITGSSSDLAAGSNVTLTINGQTYVAAVLADGSW
                     SVGVPAADVSAWPAGTVTITASGNTTAGNPVSVTHPVTVDLTAVAVSINAITVDDVIN
                     AAEKGAALTLSGSTSGVEAGQTVTVNFGGKTYTASVATNGSWSTTVPAADMAALRDGD
                     ASAQASVSNVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTA
                     EAGQTVTVTLNGENYTGTVQTDGSWSVSVPQADVSALTASNYTVSAAVNDKAGNPASV
                     NHNLTVDTSVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFT
                     TVLDASGNWSVGVPASVVSALANGTVTINASVTDVAGNSGSATHQVTVNTGLPTITFN
                     AISGDNVLNADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVS
                     DLAALGQANYTVSASATSAAGNTASSQANLLVDSGLPDVTINTVAGDDIINAAEAGAD
                     QTISGVVTRAAAGDTVTVTLGGNTYTATVQSNLSWSVSVPTADLQALGNGDLTITASV
                     TNANGNTGSGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVL
                     TVTINSVAYSATVQADGSWSVGIPAANVSAWPAGPLTVEVAGQSSADNPVSVSHPFTV
                     DLTAVAISINTVASDDVINAAEKGTNLTLSGSTSGIESGQTVTVTFGGKTYTASVAAN
                     GSWSKTVPVADLATLPDGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDD
                     ILNATEAGSPLTISGSSTAETGQTVTVTLNGATYTGTVQADGGWSVSVPTSALGALNA
                     SNYTVSATVNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTS
                     TGGEAGDVVSVVLNGKTYTTTLDASGNWSVGVPAADVAALGSGAQTITASVSDRAGNS
                     DDASRTVTVSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQN
                     YSATTDASGNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGIT
                     INPVATDDIINASEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQPDLSWNVSVP
                     AADWQALGNGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHS
                     QAQVITGSSSGFAAGTALTVVINNQTYAATVLVNGTWSVGVPAADVSNWPAGTLNITV
                     SGANSAGTQTSITHPLTVDLTTVAISINAITPDDVINAAEKGAALTLSGSTSGVEAGQ
                     AVTVTFGGKTYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEY
                     SVDSAAPTVTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQA
                     DGSWSLTLPASDLTALANNGYTLTATVSDLAGNPGSASKGVTVDTTAPVISFNTVAGD
                     DVINNVEHTQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGL
                     ADGTVTISATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNIINAAEAGSALTLSG
                     TGTNFATGTVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSA
                     GNSATASRSVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFG
                     GKTYTATVAANGTWALNVPAADLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAP
                     TVTIATVAGDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQSWSATVGSGGSWSVF
                     IPAQQFAGLSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAE
                     HGSSLVISGTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVI
                     NASVSNAIGNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSL
                     TAALASNETAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGA
                     TDSQNVVIDTTAPDPAVKTIAISAITTDTGLITNDFVTSDTTLAVSGTLGATLSAGEF
                     AQISIDGGTTWQNLSVSGLTWSYLDGRTLTDGNYNYQVRVIDTAGNIGATASQIVTVD
                     TTAPLASKTIAIASISDDTGLSSSDFVTRDTTLTVRGTLGAALAADERAQISLDGGVT
                     WTTLTVIGTSWSYADGRTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSI
                     TITGISDDTGTSSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTLAADG
                     LNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATGSQSAQIDTVNPAQVLTIASISTDTG
                     SSATDFITSDTSLTLTGSLGAGLASGEVAQISLDGGATWTTLTTNGTQWTYTDSRTLT
                     DGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPGIVSYTDDVGQRQGTLSSSQAT
                     DDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSAR
                     VVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVV
                     INGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNANI
                     SNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKT
                     ALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTV
                     GTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATV
                     GGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNTTN
                     TFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPVAV
                     GATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVGGASNWTQSALGGSQSGT
                     TSGVAAMDYDWDGAVDVLVTKQSGSVFLIRNTNTVSYGTSLHLRITDPNGINVYYGNT
                     VKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNID
                     QTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGW
                     VYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSNFN
                     DTLTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTW
                     EGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQ
                     IDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            22154..24316
                     /locus_tag="ACHOQQ_RS06770"
                     /old_locus_tag="pgaptmp_001354"
     CDS             22154..24316
                     /locus_tag="ACHOQQ_RS06770"
                     /old_locus_tag="pgaptmp_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HGU4469627.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADVEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIVATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            24313..25488
                     /locus_tag="ACHOQQ_RS06775"
                     /old_locus_tag="pgaptmp_001355"
     CDS             24313..25488
                     /locus_tag="ACHOQQ_RS06775"
                     /old_locus_tag="pgaptmp_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HGU4469628.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            25485..25892
                     /gene="cueR"
                     /locus_tag="ACHOQQ_RS06780"
                     /old_locus_tag="pgaptmp_001356"
     CDS             25485..25892
                     /gene="cueR"
                     /locus_tag="ACHOQQ_RS06780"
                     /old_locus_tag="pgaptmp_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGU4469629.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(25896..26099)
                     /locus_tag="ACHOQQ_RS06785"
                     /old_locus_tag="pgaptmp_001357"
     CDS             complement(25896..26099)
                     /locus_tag="ACHOQQ_RS06785"
                     /old_locus_tag="pgaptmp_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469630.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIN"
     gene            complement(26096..26518)
                     /locus_tag="ACHOQQ_RS06790"
                     /old_locus_tag="pgaptmp_001358"
     CDS             complement(26096..26518)
                     /locus_tag="ACHOQQ_RS06790"
                     /old_locus_tag="pgaptmp_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469631.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
BASE COUNT         6071 a         7847 c         7871 g         4734 t
ORIGIN      
        1 tgcaccgctg ccacaatggg cgaacgcccc agcggtaacg ccagttgccc aattatcctt
       61 acaggaaagt atattacgcg cctttgcgcg aaatcccgac gtcactcaac aggcggcgca
      121 gataggcatt ggcgaagcgc aaattgatga agccaaaagt gcctggtatc cgcacgttgg
      181 attaaccggc aacgcggggc cgtcccgaca aacggactcc agcggcaggc ttgataacaa
      241 cgtttcgtat ggcataaccc tgacacagct ggtgtatgac tttggtaaaa ctaacaacga
      301 tatcaatctg caaactgccg cccgtgacag ctaccgcttt aaattgatgg caaccttaac
      361 cgatgttgca gagaaaacgg cgactgccta tatggaagtc agtcgttatc aggctttgtg
      421 cgatgcggct caacgcaata ttcactcgct ggaaaacgtc tacaacatgg cggcattgcg
      481 cgctaacgct ggcctgaact catcgtcgga tgaattacag gcccagacgc gtattgccgg
      541 aatgcgctca acgctggagc aatatcaggc gcagatggtg agcgccaaag cgcaactggc
      601 ggtgctcact ggcgtacagc cggaggcgat agccgcgcca ccggctgaat tagccgagca
      661 gccggtatcg ctaaaaaata ttgattacca gtccatcccg ctggtgctgg cggcagaaaa
      721 cttacgccag tcagcacagt acggcgtgga aaaaacgaaa gcgcaatact ggccaacgct
      781 cagtattcag gggggtaaaa cgcgctacca gaccagcgac cgctcgtatt gggatgatca
      841 gctacaactg aacgttaacg cgccgctgta tcagggcggc gcggtttctg cccaggtgca
      901 acaggccgaa gggcaacaaa aaatctctgc ctcgcaggtc gaacaggcaa aactggatgt
      961 gctgcaacga gcgtctgtgg catatgcgaa ctggaccggc gcacggggtc gtgaagaagc
     1021 cggtttagcg caatccgaaa gtgcgcacaa aacgcgagat gtgtaccaaa atgaatataa
     1081 attgggtaaa cgcagtctga atgatctatt aaccgtcgag caagatgtct ttcaggcgca
     1141 atcggctgaa ataaatgcca attatgatgg ctgggtcgcc gccgtaaatt atgccgctgc
     1201 ggtgaataac cttattccat tagcgggaat taaacagggc ttatacaacg acttacccga
     1261 cttgaaataa tcctgccatt attactagtg ggccgttgtt aagtgattac ctttgtctta
     1321 tttagataaa tggactcgct aaggatgtgc gccatttatc aggaatgcca ttatgagcct
     1381 gattattgat gttatttcgc gtaaaacatc cgtcaaacaa acgctgatta atcctggcga
     1441 cgtcacggtt gttatttatg agccttccgt ggtgcaggtt catgctcagg cctctgccgt
     1501 tgcgcgttac gtccgtgaag gaaatgacct gctgatctat atgcaggacg gcacggtgat
     1561 ccgctgcaac ggttatttcc tgcaagcggc gaatacagct gaacaatcgg aactggtgtt
     1621 tgccgatggt caacagctaa cccatatcac ctttgccgat actgctgcgg gtggattagc
     1681 ccccgtagaa ctgactgccc agaccactgc gattgaaagc attgcgccat ttcttgatac
     1741 cgttgctcag accagcgctt tcccgtgggg ttggctggcg ggggcggcgg taggtggtgg
     1801 cgcgcttggt gcactgctgg caagcggtgg cgatggcgac tcgaaaacag aagtgattaa
     1861 taaccctacg ccacccgctg agcctggcaa cgccacacca tcatttttag ttaccgataa
     1921 tcagggcgat cagcgcggca ttctcgccac caatgacatc accgatgaca ccacgccaac
     1981 ctttagcggc agcgggcagg cgggggcgac tattcagatt aaagacagta acggcaatac
     2041 tattgccagt actcaggtag acaacaacgg tcactggagt gtctcgttac ccacgcaaag
     2101 tgcaggtgaa catacctggt cagtggtgca aattgtcggc agtaccatca ctgacgccgg
     2161 ttcgataacg ttaaccatcg acaatagtca ggccagcgtg caggttgcca ccaccgcagg
     2221 cgataacatt attaacgcca gcgaacaggc cgccgggttt acgctttctg gcaccagtag
     2281 ccatctggcg cagggaacag aactcaccgt cacacttaac ggcaaaacct acaccacaac
     2341 tgtaggggct aacggtgcct ggagcgtgca ggtgccgacc gccgatgcac aggcgttagg
     2401 cgaagggaat caggcggtac tggtcagtgg gaaagacacc acaggcaata cggtcaccgg
     2461 cgcgcagctg ctaacggtcg atacccaacc gccaacgctt gccatcaaca ccatcgccca
     2521 ggacaacatc atcagtgctg cggaacataa cgtcgcgctg gtactgagcg gcacgtcgaa
     2581 tgcagaagcg gggcaaaccg taacactgac cgtcaacggg aaaagccata cagcaaccgt
     2641 cggtagcgac ggaacctggc aagtgacgct gcctgccacg gaagtccagg cactggcgga
     2701 gggtaattac gctgtcaatg ccagtgtcag cgatcgggca gggaacacca ccagccacag
     2761 cgcgaatttc acggtagaca cctcagcacc cgtggtcagt gttaataccg tggcgggcga
     2821 cgatattctt aataatgccg agcaggccgt cgcgcagatc atctccggac aagttagcgg
     2881 tgcttctcct ggcgatacgg taacggtgaa attgggcact catgtcctga cgggcatcgt
     2941 gctggcagat ggcagctgga atgtggcgtt ggacccggcg gtaacccgca cgctggatcg
     3001 cggagccaat acgattttcg tcaccgtgac agatgctgca ggaaatactg gcgcggcgtc
     3061 tcgagcaatc acgctggtcg gtgtttctcc gttgatcacc attaacaccg tctccggcga
     3121 tgacattatc agtggcgcag aaaaaggtgc gccactgacc cttaccggta gcactcaaca
     3181 ggctgagaca ggacaaaccg tcacagtaac cctggctgga cagagtttta ccactaccgt
     3241 gcaggccgat ggctcctgga gtctgacggt acctgccgcc gcgatgggaa atctgcctga
     3301 cggcgaggtg gcgattaccg cttctgtgac ggatctcagc ggcaataccg gcaacacctc
     3361 ccgcaccatt accgtcgaca gccaggcccc ggccttaagc attgatccac tgaccgctga
     3421 taacatcatt aacgtcgccg aaagcgggca ggatctgccc atcaccggca ccaccgacgc
     3481 ccagccaggg cagacggtaa cggtgacgct taatgggcag acgtatcagg gcgtcgtgca
     3541 gccagacggc acctggagcg tgaccgttcc cgccgccaac gttgacgcgc tggctgacgg
     3601 taacgccacg gtcaccgcca gcgtgaacga tgtcgccggt aacccgacca gcgtctcacg
     3661 cgtggcgctg gtcgatgcca cgccgccggt ggtgaccatt aatccggtgg cgaccgataa
     3721 tgtcattaac acgccggaac atatccaggc gcaaattatc agcggcacgg tcactggcgc
     3781 tcaggcgggc gatatcgtca ccgtgacgct gaataatgtg gattacacca cggtggtgga
     3841 tggttccggc aactggagtc tgggcgttcc ggcctcggtg gtcagtgggc tggcggacgg
     3901 cagttatcct gtcagcgtct cggtaaccga caaagccgga aacacgggca gccagtcatt
     3961 gaccgtcacg gtcaataccg ccgcgcccct tatcggcatt aacagcattg cgggcgatga
     4021 tgtgattaac gccagcgaaa aaggggccga tctccagatt accggcacca gcgatcagcc
     4081 tgttaacacc gccatcaccg tgacgctgaa cgggcaaaat tacaccacca cgaccgacgc
     4141 ctccggcaac tggagcgtca ccgttccggc atcggcggta accgcactgg gccaggccaa
     4201 ctacacggta acggcggcgg tgaccagcag tatcggcaat agcaccaccg ccagccataa
     4261 cgtgctggtc gacagcgcgc tgcccggcgt gaccattaac ccggtggcaa ccgacgatat
     4321 tattaacgcc gccgaagcgg gcgtggcgca aaccatcagc gggcaggtga caggagccgc
     4381 cgttggcgac gcggtaaccg ttacgctggg tggcaataac tacaccacca ccgtgcaggc
     4441 cggcttaagc tggagcgtgg acgttccggc ggacgctatt caggcgctgg gtaatggcga
     4501 tttaacggtt aatgcctcgg tcaccaatca aaacggcaac accggcagcg gcacgcggga
     4561 tatcaccatc gacgccaatc tgcccggcct gcgggtcgat acggtggcgg gcgatgatgt
     4621 ggtcaatatc atcgagcacg ggcaggcgct ggtggtcacc ggcagcagct tggggcttgc
     4681 tgaaggcacg ccgcttaccg tcacgattaa cgatgtggaa tacaccactg cggtgcaggc
     4741 cgatggcagc tggagcgtgg gcatcacggc ggcgcaggtt agcgcctggc ctgcgggggc
     4801 ggttaatatt gccgtatccg gggaaagtag cgccggaaat ccgataagca ttacgcatcc
     4861 ggtaacggtg gatctcactc cggcggcgat caccatcaac accatcgcca cggatgacgt
     4921 gattaacgct gcagaaaaag gggctaattt aactctttcc ggcaccacca ctaacgtaga
     4981 agcagggcaa accgtcactg tcacctttgg cggtaaaaat tacaccgcca gcgtggcggg
     5041 cgatggtagc tggactgcca ccgttcccgc cgccgatctg gcagcattac cagagggcag
     5101 cgcctccgcg caggccagcg tcagcaatat caacggcaat agcgcctcgg cggtgcacag
     5161 ctacagcgtc gacagcagcg cgccaaccat cattatcaat accgtcgcca gcgacaatat
     5221 cgtcaacgcc agcgaagccg atgcgggtgt gacggtgagc ggcagcacca ccgctgaagc
     5281 ggggcagatt gttacggtaa cgctcaatag cccgaccgtg cagacgtatc aggcaacggt
     5341 gcaggcggac ggcagctgga gcatcaatat tccggcggca gatcttgagg cattgaccga
     5401 tggcagccac accctgaccg ccacggtcaa tgacaaagcg ggcaacccgg cgagcaccac
     5461 gcataatctg gcggtggatc tcaccgttcc ggtactcacc atcaacacca ttgcgggcga
     5521 tgacattata aacgccaccg aacacgggca ggcgctggtg atttccggtt ccagcactgg
     5581 cggagaagcg ggagatgtcg tcaccgtcac gctaaacagt aaaacctaca ccacgacgct
     5641 ggatgcctcc ggcaactgga gcgtcggcgt tccggcggcg gatgtcacgg cgctgggtag
     5701 cggcccgcaa accgtgactg cgaccgttac cgatgccgcc ggtaacagcg atagtgagac
     5761 gcacacggtc accgttaacc ttaccgcgcc gaccattggc attaacacca tcgccaccga
     5821 tgacgtgatt aacgccaccg aaaaaggcgc ggatctgcaa atttccggca ccagtaatca
     5881 gccagcaggc accacaatta cgctgacgct gaacgggcaa aattacactg ccaccacaga
     5941 tgcttccggc aactggagca ccacagtgcc agcttccgca gtcggtgcgc tgggcgaagc
     6001 cagctacacg gtgacggcga acgtcaccga cagcgcgggc aacagcaact ccgccagcca
     6061 taacgtgcag gtgaataccg cgctgcctgg cgtcaccctt aatccggtag cgagcgacga
     6121 tattatcaac gctgccgaat cgggcgtggc gcagaccatc agcggccagg tcacgggcgc
     6181 ggcggcgggc gatacggtta ccgtaacgct tggcgggaaa acctacaccg ctaccgtgca
     6241 ggggaattta agctggagcg tggacgttcc ggcggcggat attcaggcca tcggcaatgg
     6301 cgagctgacg gttaacgcct gggtaaccaa cggcgttggc aataccggca gcggttcgcg
     6361 cgatattact atcgacgcca acctgcctgg cctgcgcgtc gataccgtgg cgggcgatga
     6421 tgtggttaac agcatcgaac acggtcaggc actggtgata actggtagca gcagcgggct
     6481 ggcggcgggc gctgcgctga cggtggtgat caataacgtc acctatggcg cgacagtatt
     6541 agccgatggc acatggagcc ttggcgttcc ggcggcaaat gtaggcaact ggcctgcggg
     6601 tacggtgaat atcacggttt caggtgctac tagcgcagga aacccggtca ccatcaccca
     6661 tccagtcacc gtcgatctgg cagctgtggc gatctccatt aacaccgttt ccggcgacga
     6721 tgtgattaac gccgccgaaa aaggggcaga tttaaccctt tccggcagca cctccggcgt
     6781 ggagccaggg caaacggtca ccgtcacctt tgacggcaaa acctacaccg ccaccgtagc
     6841 aggcgatggt agctggacaa ccaccgtccc tgccgccgat ctcgccgcat tacgcgacgg
     6901 cgacgccacg gtacaggcca gcgtcagcac tattaacggc aacacggctt cggcaaccca
     6961 cgtctacagc gtcgatgcca cgaccccgac gcttaccatt aacaccatcg ccaccgacga
     7021 tattctgaac gccgccgagg cgggtaatcc gctaaccatc agcggcagca gtaccgccga
     7081 agcggggcag acggtaaccg tcacgcttaa tggcgtgact tacaccggca ccgttcaggc
     7141 ggacggtagc tggagcgtca gcgtgccgac ggcggatctc agcaatctga ccgccagcca
     7201 gtacaccgtc agcgcctcgg taaacgataa agcgggtaac ccggtgtccg ccactcacgg
     7261 gctggcggtg gatctcaccg tgccggtgct gaccattaac accgtctcgg gcgatgacat
     7321 tattaacgct actgaacacg ggcaggcgct ggtgatctcc ggttccagta ctggcggcga
     7381 agcgggcgat gtcatcaccg tcacgttaaa cagtaaaacc tacaccacca cgctggacgc
     7441 ttccggcaac tggagcgtcg gcgttccgct gtctgatgtc accgcgcttg gcagcggccc
     7501 gcaaaacatc acggccacaa ttaccgacgc ggcaggcaac agcgatgacg ccagccgcac
     7561 ggtcaccgtg aatctcgccg cgccaaccat tggcatcaac accatcgcca ccgatgacgt
     7621 gattaacgcc acggaaaaag gcgcggatct gcaaattacc ggtaccagta atcagccagc
     7681 gggcaccacg ataacggtga cgctgaacgg gcaaaattac accgccacca cagatgcttc
     7741 cggcaactgg agcgccacgg tgccagcctc ggcagttagc gcattgggtg aagctaacta
     7801 cacggtaacg gcaaacgtca ccgacaaggc aggcaacagt aattccgcca gtcataatgt
     7861 gctggtcaac agcgccttgc ctgccgttac cattaacgcg gtggcgactg acgatattat
     7921 caatgccgac gagtcggaca atgcgcaaac catcagcggc caggtgacgg gggccgcggc
     7981 gggagatacc gttaccgtga cgctgggcgg caacacctac accgccacgg tgcaggctaa
     8041 tttaagctgg agtatcagcg ttccggcggc ggatattcag gcgctgggta acggcgacct
     8101 gacaattaat gccttggtca ccaatggcgt gggcaacacc ggcagcggct cgcgcgatat
     8161 cacgatcgac gccaatctgc ctggcctgcg ggtcgatacc gtggcgggcg atgatgtgat
     8221 taacagcatt gagcacaatc aggcgctggt gatcaccggc agtagcaccg ggctgacggc
     8281 gggaacggcg ttaacggtcg agattaacaa cgttacttac gctgccacgg tgttagccga
     8341 cggcacgtgg agcctcggtg ttccggcggt agacgtcagc aactggccag cgggtacggt
     8401 gaatatcacg gtaagcggca ccaacagcgc cggaacaacc tccaccatta cccatccggt
     8461 caccgtcgat ctggcggcgg ttgccatcac cattaacacc ctttccggcg atgatgtgat
     8521 caacgccgtc gaaaaaggcg aaacgctggt ggtgagcggc agcaccagcg gtgtggaagc
     8581 cgggcagacg gtgaccgtca cctttggtgg caaaaattac accactaccg tggaagccaa
     8641 cggcagctgg acggtgaatg tgccgcctgc cgatctcgct gcgctgccgg atggcgcggg
     8701 caacgtgcag gcgagcgtca gcaacatcaa cggcaataac gcccaggcgg atcgcgcgta
     8761 cagcgttgat gccaccgcgc cacttgtgac catcaacacc atcgccagcg acgatactct
     8821 taacgtgagc gaagcgggcg cggggataac catcagcggt accaccacgg cgcaggccgg
     8881 gcagacgctc accgtcacgc tcaataacaa cacgtaccag accaccgttc aggcggatgg
     8941 cacctggagc gtgaatgttc cggcagcgga tttaagcgga ttaaccgcca gcagttacac
     9001 cgtgaccgcc acggtgagcg acaaagcggg taacccggca agcgccgacc acgcgctggc
     9061 ggtggacgtt accgcaccgg atctcaccat taataccgtc gcgggcgatg acattatcaa
     9121 cgccatcgaa cacggtcagg cactggtcgt cagcggaacc agtacgggcg cggcggcggg
     9181 cgatgtggtc acggttaacc tgaacggtaa aaactacacc accacgctgg acgcctccgg
     9241 taactggagc gtgggcattc cggcggcgga tgtcacggcg ctggcaaccg gcagccagac
     9301 gatcaccgcc agcctgagcg atcgcgcggg caacagcgac agcactaccc ataatgtgac
     9361 cgttgatctc agcggcccga cgctgaccat cagtatcgtc tccggtgatg acattattaa
     9421 taatgcggag aaaacccagg atctaatcat cagcggcggc tccagcggcc tggcgaccgg
     9481 cactacggtc accgtgatgt tgaacggcct ggcgtacagc gcaactaccg acggttctgg
     9541 caactggagc gttactgtgc ctgcttcggc ggtcggcgca ctgggcgagg cggtgtacca
     9601 gattagcgcc agcgctaccg acagcgcggg caacagcggc atcaccaccc atacggtgaa
     9661 tgttgaatcg ctcctgccag gggtgatcat taataccgtg gcgggcgatg acattatcaa
     9721 cgccgctgaa attggtgtta accagaccat cagcggacag gttacgggga cggcagcagc
     9781 cggagatacg gtcactgtca cgctcggcgg taatcaatac gtcgccaccg tacagccaga
     9841 tttaagctgg agcgtcagcg taccggcgaa cgttttgcag gcgctgggca acggtgagtt
     9901 gaccatcagc gcctcggtga ccaattccgc aaataatact ggcaccacaa cgcacgacat
     9961 cgtgatagat gccaacctgc caggcctgcg ggtcgatacc gtggcaggcg atgatgtgat
    10021 taacagcatc gagcacactc aggcgttggt gatcaccggt agcagtagcg gactggcggc
    10081 gggcgcggcg ttgacggtgg ttatcaatag cgtcacctac ggcgcaacgg ttttagcgga
    10141 tggtagctgg agcgttggtg ttccggcggc agatgtcaca aactggcctg cggggacggt
    10201 caatattgcc gtctctggca ccaacaccgc cggaacgaca accagtatta cccatccggt
    10261 gaccgtcgat ctcgcagccg tggcgatcac catcaacccc ctttccactg acgatgtgat
    10321 taacgccgct gagaaaggct ctgatttgca gctctccggc accacctccg atgtggaagc
    10381 ggggcaaacc atcaccgtta tcttcggcgg caaaagctac accaccacgg ttgccgcgga
    10441 taatacctgg gggctgacaa tcccagccgc cgatctcgcc gctttaccgg atggcgcagc
    10501 aaacgtccag gccagtgtca gcagcgtcgc aggtaacagc gcccaggcaa cgcatgttta
    10561 cagcgttgat gccaccgcgc cctccgtcac catcaacacc atcgccgcag acgatattct
    10621 taacgccgct gaagcgggat cggcgctgat catcagcggc accagcacga cagaagccgg
    10681 gcagacggta accgtgacgc ttaacggcgt taattacagc ggcaacgtgc aggctgacgg
    10741 gagctggagc gtcagcgtac caactggtga tttagctaac cttaccgcca gctcgtatac
    10801 cgttaacgcc tcggtcagcg acaaagccgg aaatccggct tcggcaacgc ataatctgac
    10861 ggtggacctt gccgctccgg tcgtcaccat caacacggtg gcgggcgatg acatcatcaa
    10921 cgccacggaa cacggacagg cgcagatcat cagcggctcg gcaacgggcg cgactaccgg
    10981 taatacggtt tccgtgacga ttggcacgac cacctatacc accgtgctgg acgccaacgg
    11041 caactggagc atcggcgtgc ctgccagcgt gatttccgca ctggcgcagg gcgatgtgaa
    11101 cattagcgcc acggttaccg actccgcagg caacagcggc acagcctcgc acgttgtcag
    11161 cgtggcgctc ggcgctccgg tgctcgccat taacaccatt gccgtcgatg acatcatcaa
    11221 cgccacggag aaaggcgcgg atctggcgat ttccggcacc agcgaccagc ctgcgggcac
    11281 gcagattacc gttacgctca acgggcaaaa ttacaccacc acagccgatg cttccgggaa
    11341 ctggagcgtg accgttccgg cgtctgcggt tggtacgctt ggtgaagcga cttataccgt
    11401 gaccgcctcc gccactgacg ccgatggcaa cagcggttcc gccagccata acgtacaggt
    11461 taataccgcg ctgccgggcg tcaccattaa cgtggtggca acggacgata ttattaacgc
    11521 cgccgaagcg ggcgtggacc agaccatcag cgggcaggtg acgggtgcgg cggcaggcga
    11581 cacggtgacc gtcacgctcg gcggggcgac ttacactgcc acggtgcagg cgaatttaag
    11641 ctggagcgtc gatgttccgg cctccgcgtt acaggcgttg ggcaacggtg agctgaccat
    11701 ttcggcttcg gtaacgaaca gcgtaggcaa taccggcaac ggcacgcgtg aaatcaccat
    11761 cgacgcgaat ctgccaggtc tgcgggtcga taccgtggcg ggcgatgatg tggtcaatat
    11821 catcgagcac gggcaggcgc tggtgattac cggcagcagc tccgatctgg cagcgggcag
    11881 caacgtcacg ctgaccataa acgggcaaac gtatgttgcg gcggtgctgg cggatggctc
    11941 atggagcgtc ggtgttccgg cggcagatgt cagcgcctgg cctgcgggga cggtcacgat
    12001 tacagccagc ggcaacacca ccgccggaaa cccggtgagc gttacgcatc cggtgactgt
    12061 cgatctcacg gcggtggcgg tcagcatcaa cgccattacc gtcgatgatg tgattaacgc
    12121 cgctgaaaaa ggcgcggcgt taacgctctc cggcagcacc tctggcgtcg aagccgggca
    12181 aacggtcacc gtgaactttg gcggaaaaac ctacactgca agcgtggcga cgaacggttc
    12241 ctggagcacc acggttccgg cggcagatat ggcggccctg cgtgatggcg atgccagcgc
    12301 acaggccagc gtcagcaatg ttaacggcaa cagcgccacc actactcacg cttacagcgt
    12361 cgatgccagc gcgccaacgg tgaccattaa caccattgcg ggcgacgaca ttcttaacgc
    12421 cgccgaagcc ggagcggcgc tgaccatcac tggcagcagc acggcggaag cggggcagac
    12481 ggtgaccgtc accctcaatg gcgaaaacta tactggcacg gttcaaacgg acggtagctg
    12541 gagcgtcagt gtgccgcaag ccgacgtaag cgccctgacc gccagcaact acaccgtgag
    12601 cgcggcggtg aacgacaaag ccggaaaccc ggcctcggtt aaccacaacc tgacggtgga
    12661 tacttctgtt ccggtcgtca ccatcaacac ggtggcaggc gatgatgtga tcaacgcgac
    12721 ggaacacgcc caggcacaga tcatcagcgg atccgccacg ggtgcggcaa ccggtagcac
    12781 cgtgacggtg actatcggca caaatacttt caccacggtg ctggatgcca gcggcaactg
    12841 gagcgtcggc gttccggcaa gcgtcgtctc ggcactggcg aacggcacgg tgaccattaa
    12901 cgccagcgtc accgatgtcg ccggtaacag cggcagcgcc acgcatcagg tgacggtcaa
    12961 taccgggctg ccgaccatta cttttaacgc catcagcggc gataacgtcc tgaacgctga
    13021 tgaaaaaggc cagccgttga ccatcagcgg cggcagtacg gggctggcga cgggcgcgca
    13081 ggtcaccgtc acgctcaacg gtcacaacta cagcgccacc accgacgcgt cgggcaactg
    13141 gaccttaacc gtgccggtga gcgatctggc ggcattaggt caggccaact atacggtcag
    13201 cgccagcgcc accagtgcag caggcaacac cgccagcagc caggcgaatt tactggtcga
    13261 cagcggcctg ccggacgtca ccatcaacac cgtggcaggc gacgatatta tcaacgccgc
    13321 cgaagcgggg gccgatcaaa ccatcagcgg ggtggtgact cgcgccgccg ctggcgatac
    13381 ggtcaccgtg acgctgggcg ggaacactta caccgctacg gtacagagca acttaagctg
    13441 gagcgtcagc gttccgacag ccgatctcca ggcgttgggc aatggtgatt tgaccattac
    13501 cgcctcggtc accaacgcta atggcaacac cgggagcggc acgcgggata tcaccattga
    13561 tgccaacctg ccggggctgc gcgtagatac cgtggcgggc gatgatatcg tcaacagcat
    13621 cgagcacggg caggcgctgg tgatcaccgg cggcagtagc ggcctgaatg caggtgctgt
    13681 gctgacggtt accatcaaca gtgtggcgta ttccgccacg gtgcaggcgg acggaagctg
    13741 gagcgttggc attccggcgg caaacgtcag cgcctggcct gcggggccgt taaccgttga
    13801 ggtagccggg caaagcagcg ccgataaccc agtcagcgtc agccatccgt tcaccgtcga
    13861 tttaacggcg gtggcaatca gcatcaatac ggtcgccagc gacgacgtga ttaacgccgc
    13921 cgagaaaggc accaatctga ccctttccgg cagcaccagc gggattgaaa gcgggcaaac
    13981 cgtaaccgtc acctttggcg gcaaaaccta caccgcaagc gtcgccgcca acggaagctg
    14041 gagcaaaacg gttccggtgg cagatctggc aactctgcca gacggcgcgg cgaacgtgca
    14101 ggccagcgtt agcagcgcga gcggtaacag tgcctcggcg acccatgcgt atagcgttga
    14161 cgccagcgcg ccgacgctca ccattaacac catcgccagc gacgatatcc ttaacgccac
    14221 ggaggccgga agcccgctca ccatcagcgg ctccagcacc gccgaaaccg ggcagacggt
    14281 caccgtcacc cttaacggcg caacctacac cggcactgta caggcggacg gcggctggag
    14341 cgtcagcgtt cctacttcag ccctgggcgc gctcaacgca agcaattaca ccgtcagcgc
    14401 cacggtcaac gacaaagcgg gcaaccccgg cagcgccagc cacaatctgg cggtagatac
    14461 caccgcgccg gttctcacca ttaacaccgt ggcgggcgat gacatcatca acgacgccga
    14521 acacgcccag gcgctggtga tctccggtac cagcacgggc ggggaagcgg gcgatgtggt
    14581 gagcgttgtg ctcaatggca aaacctacac caccacgctg gatgcctccg gtaactggag
    14641 cgtcggcgta ccggcggcgg atgttgcggc gctgggtagc ggcgcacaga ccatcaccgc
    14701 cagcgtcagc gatcgggcag gcaacagcga cgacgccagc cgcaccgtga ccgtcagcct
    14761 cagcgcgcca gtgattagca tcaacaccat cgcgggcgat gatgtgatca acgcgacgga
    14821 aaaaggatct gatctggcgc tttctggcac cagcgatcag cctgcgggta cggcgatcac
    14881 cgtcaccctg aacggacaaa actacagcgc caccacggat gcctccggca actggagtgt
    14941 taccgtgcct gcctcggcgg tcagtgcgct gggcgaagcg acctacagcg tgacggcgag
    15001 cgtcaccaat gctcagggta acagcagcac cgccagccat aacgtgcagg ttaataccgc
    15061 gctgccgggc atcaccatta atccggtggc gacggacgat attatcaacg cttcagaggc
    15121 aggcagcgcg caaactatca gcggccaggt aaccggtgcg gcggcgggca gcaccgtcac
    15181 cgttgaactg ggcggtaaaa cttacaccgc caccgtgcag ccagatttaa gctggaatgt
    15241 aagcgtacct gccgccgact ggcaggcgct gggtaacggt gagttaacgg ttaatgcctc
    15301 ggtgaccaac gccgttggca ataccggcag cggcacgcgc gatatcacca tcgatgccag
    15361 tctgcccggc ctgcgggtgg ataccgtcgc gggagatgat gtggtcaata tcatcgaaca
    15421 cagtcaggcg caggtgatca ccggcagcag ctccggcttt gccgcaggca cggcgttgac
    15481 ggtggtgatt aacaaccaaa cttacgccgc cacggtgctg gtgaatggta cctggagcgt
    15541 cggcgttccg gcggcggatg tcagtaactg gcctgcggga acgctgaata ttaccgttag
    15601 cggcgcgaac agtgccggaa cgcaaaccag cattacccat ccgctgactg ttgatctcac
    15661 taccgttgcc atcagcatta acgctattac ccctgatgat gtgattaacg ccgccgaaaa
    15721 aggcgcggca ttaacgctct ccggcagcac gtccggcgtc gaagcggggc aagccgtcac
    15781 cgtcaccttt ggcggtaaaa cctacaccac cacggtggcg gcaaacggta gctggagcac
    15841 cactgttccg gctgcagatt tggcggcgct gcgtgacggc gatgccagcg cccaggtgcg
    15901 ggtgactaac gtcaacggca acagcgccac agccacgcat gaatacagcg tcgatagcgc
    15961 cgcgccaact gtgaccatca acaccattgc cagcgataac atcatcaacg ccagcgaagc
    16021 ggcggcgggc gtaacggtgt ccggcaccag cacggcgcaa accgggcaga cgctcaccgt
    16081 cacgcttaac ggcactaact accagaccac ggtgcaggca gacggcagtt ggagcttaac
    16141 gctgcccgcc agcgacttaa ccgcactggc caataacggc tacaccctga ccgctacggt
    16201 cagcgatctg gcgggtaacc ctggcagcgc cagcaaaggt gtgaccgtcg ataccaccgc
    16261 gccggtgatc agctttaaca ccgtggcggg cgatgatgtg attaacaacg tcgaacacac
    16321 tcaggcgcag attatcagcg gcaccgccac gggcgcggtc gcaggcgacc gcctggtggt
    16381 gaccatcgcc gggcagcagt atgtcaccag caccgatgcc agcggcaact ggagcgtcgg
    16441 cgtgcctgcc agcgtgattt ccggccttgc ggacggcacg gtgaccatca gcgccaccat
    16501 taccgatagc gcaggcaaca gcagcacgca gacgcacaac gtacaggtga acacggcggc
    16561 agtgtcgctt tcggtcagca ccatcagcgg cgataatatt attaacgccg ccgaagcggg
    16621 cagtgcgctg accctgagcg gcactggcac taatttcgcg acaggtacag tggtgaccgt
    16681 gttgcttaac ggcaaaggct acagcgccac cattcagagc aacgggagct ggagcgtgaa
    16741 cgtgcctgcg gcggatgttg cggcgctcag tgatggcacc agctacacgg ttagcgcctc
    16801 cgctcaggac agtgccggaa acagcgccac ggcttcgcgc agcgtggcgg tggatctcac
    16861 cgcgccggtg atcagcatta acaccgtttc gacggatgac cgactcaacg ccgccgaaca
    16921 acagcagccg ttaaccctta acggctcgac cagcgcggaa gtgggacaga cggtcaccgt
    16981 cacctttggc ggcaaaacct ataccgccac ggttgctgcc aatggcacct gggcgctgaa
    17041 cgtgcctgcg gcagatctgg ccgcgctcgg gcagggcgcg cagaccatta ccgccagcgt
    17101 gaacgatcgc gccggtaacc ccggacaggc cacgcacgcc ctgaccgtcg ataccgttgc
    17161 gccaacggtc accatcgcca cggtggcggg tgacgatatt atcaacaacg ccgagcagct
    17221 tgccgggcag accatcagcg gcaccaccac cgccgaagta ggccagacgg tgaccgtcac
    17281 ttttaatggt cagagctgga gcgcaacggt cggcagcggc ggaagctggt cggtatttat
    17341 tccggcgcag cagtttgccg gattaagcga cggcagctac accatcagcg cgacggttag
    17401 cgatcaggcc ggaaaccccg gcagcgccag ccgtggcgtg acgctgaacg gcgacgtgcc
    17461 caccgtcacc atcaacacct ttgcgggcga tgatgtggtg aatgcagcag aacacggctc
    17521 gtcgctggtg atcagcggca ccaccaccgc gccggtcgga cagacgctga ccctgacctt
    17581 aaacggcaaa acctacacca ccacggtaca gaccggcggt agctggagct acaccctagg
    17641 cagcgccgat gttaccgcgc tggcggacgg caacgcctac gtgattaacg cctcggtgag
    17701 caatgccatt ggcaacaccg gtagcagtaa tcacaccatt accgtcgatc tcagcgctcc
    17761 ggcgatgggc attaatatcg attccctgca agccgacact ggccttagcg ccagcgactt
    17821 tatcaccagc gtcagtccgg tagtggtcaa cggctcgctc accgccgcgc ttgccagtaa
    17881 cgagacggcg caaataagta tcgacggcgg cacaacctgg accacgctta ccgttaccgg
    17941 cacgacctgg cgctataacg acagccgcac gctgaccgat ggcaactatc tctatcaggt
    18001 gcgggtgatt gacgcagcgg gcaacgttgg cgcgaccgac agccagaatg tggtgatcga
    18061 cactaccgcg ccagatcccg cggtgaaaac catcgccatc agcgcgatca ccaccgatac
    18121 gggcttgatc actaacgatt ttgtcaccag cgacacgacg cttgcggtga gcggcacgct
    18181 gggggcgacg ctttctgccg gtgagttcgc gcaaatcagc attgatggcg gcaccacctg
    18241 gcagaatctt tcggtaagcg gtttgacctg gagctatctt gacgggcgta cgctgaccga
    18301 tggcaactac aactaccagg tgcgggtgat cgacaccgcc ggaaatatcg gcgcgaccgc
    18361 cagccagata gtcactgtgg ataccaccgc gccgctggcg agcaaaacca tcgccattgc
    18421 gagcatcagc gatgacaccg ggttaagcag cagcgatttt gttacacgcg acaccacgct
    18481 gaccgtgcgc ggcacgctcg gcgcggcgct tgccgccgat gaacgggcgc aaatcagcct
    18541 cgacggcggc gtcacctgga ctacgttaac cgttatcggc accagctgga gctatgccga
    18601 tggtcgcacg cttactgacg gcacctggaa ctacacggtg cgggtggtgg atctggcggg
    18661 gaacgttggg cagaccgcaa cgcaaaacgt ggtggtcgat accaccagcc cggaagcggc
    18721 gaaaagtatc accattaccg gcatcagcga tgacaccgga accagcagca gcgattttat
    18781 taccagcgac accacgctta ccgtgcgcgg cgtgttgggc gcggcgctcg gcgctaatga
    18841 gttcgcacaa atcagtaccg acaacggcgc aacctgggtg aacgtgacgc tagcagcaga
    18901 cggcctgaac tggagttacg ttgacggacg aaccctcacc aacggcacca ccacctggca
    18961 ggtgcgggtg gtcgatctgg cgggcaacgt tggcgcaacc ggcagccagt cggcgcagat
    19021 tgacaccgtt aacccggcgc aggtgttgac catcgccagc atcagcaccg acacggggag
    19081 ttcggcaact gactttatca ccagcgatac ctcgctcacg ctaaccggtt cgctgggagc
    19141 ggggcttgcc agcggcgaag tggcgcagat tagccttgat ggcggcgcga cctggactac
    19201 gctcaccacc aacggcacgc agtggaccta cactgacagc cgcacgctga ccgacggcag
    19261 ctatgtttat caggtgcggg tgctggatct ggccgggaac accggtccgg tggtgtcgaa
    19321 aacggtggtg gtcgatacga ttaaccccac cgccacgcca ggtattgtgt cgtataccga
    19381 tgatgtcggg cagcggcagg ggacgttaag cagttcgcag gccaccgacg acactacgcc
    19441 gctgctgaac ggtgtacttt ccgcgccgct tgccagcggt gaagtggtct atctctaccg
    19501 taacggcctg ctgttaggtg cggtaacgat ggtcggcgca ctgaactgga cctacagcga
    19561 cagcgggctg gtcagcggtg cctataccta cagcgcgcga gtggtggatt tggcggggaa
    19621 tatcacctcc tccagtgatt ttgtcctgac ggtcgatacc tctattccga ccacgctggc
    19681 gcaaatcacc agccagacca cgcgcgatac tacgccgatt atcagcgggg tgatcaccgc
    19741 cgcgcttgcc agtgggcagt atgttgaagt ggtgatcaac ggcaaaacct acacctctga
    19801 accgggcggc gcggtagtgg tcgatccggc gcacaacacc tggtatgtac agttgccgga
    19861 taccgatgcg ctgacagttt ccgcgaccgc ctataccgtt actgcacagg taaaaagttc
    19921 ggcgggtaac ggcaataacg ccaatattag caacggcacg gtgacggtta acgcggcgat
    19981 tgattacaca ccgacctgga ccaccgccag caaaaccacc gcctgggggc tgacctacgg
    20041 cctcgactcg cacgggatgt ggacggtgct ggcaaaccag caggtaatgc aatcgactga
    20101 cccactcacc tggtcgaaga ccgcgctgac gctgtatcag agcggcaaca actacgccac
    20161 cagctccatc gccgattacg accgtaacgg cacgggcgat ctgtttatca cccgtgatga
    20221 ctacggtaca ggctatatca acggctttac caataacggc gacggcacct tttccagcgc
    20281 tattcaggtc accgtcggca ccctgacgtg gtacggctcg attgtggcat ttgataaaga
    20341 gggcgacggc tatctcgact tctggattgg cgacgcgggc gggccggact ccaacacctt
    20401 tctgtggaac aacgcaggca cgctggtagg caactccacc acgtcgaaca gcggcggtag
    20461 cgccacggtg ggcggggcgg tgacggggta tctttcgctc aacgaaggtt ctggcgtcga
    20521 tctgaacaat gacggcagga tcgacctggt tcagcacacc tataacctga acaactatta
    20581 cacgctgtct tcgctcatca accaggggaa tgggacgttt gtctgggggc agaacaccac
    20641 caataccttc ctgagcgggg cgggtagtgg cgctatgagc agcagcgttt ccatgacctg
    20701 ggccgatttc gatggtgacg gcgatatgga tctcttcctg cccgccagcc agggaagagc
    20761 taactacggc tcgctgttat tcaacaccaa cggcgtactg ggtagcccgg tggcggtggg
    20821 cgcaacggca accacctacg ccagccagtt tagcctggcg gtggactgga accatgacgg
    20881 cctgatggat atcgcccgta tcgcccagac cggacaatcg tatctttaca ccaacgttgg
    20941 tggtgccagc aactggacac aatcagccct cggcggcagc cagagcggta ccaccagcgg
    21001 cgtggcggca atggactacg actgggacgg cgcggtggat gtgctggtga ccaaacagtc
    21061 gggcagcgtg ttcctgatcc gcaacaccaa cacggtgagc tacggcactt cgctacacct
    21121 gcgcatcacc gatcccaacg gcattaacgt ctattacggc aataccgtga agctgtacaa
    21181 ctcggcggga gtgctggtcg ccacgcaaat catcaacccg cagtcgggta tgggggttaa
    21241 cgacacctcg gcgctggtca acttctacgg gctgaatgcc ggagaaacct acaacgcggt
    21301 gctgatcaaa tccaccggca ccaccgccag caatatcgac cagacggtca acaccagctg
    21361 gggcggttta caggccaccg atgccactca cgcttacgac ctcagcgccg aagcgggtac
    21421 cgcgagcaac aacggcaagt tcgtcggcac cggttataac gacaccttct ttgccaccgc
    21481 aggcaccgat acctacgacg gttccggcgg ctgggtgtac agctccggca ccggaacgtg
    21541 gctggcgaac ggcgggatgg acgtggttga tttccggctt tcgacggtgg gcgtgacggc
    21601 taacttaagc agcaccgccg cgcaggccac cggttttaac acctcgacat ttaccaatat
    21661 cgaaggcatt tccggatcga attttaacga cactctgacc ggcagcagcg gcgataacca
    21721 actggaaggg cgcggcggca acgacacgct caacatcggc aacggcggcc acgacacctt
    21781 gctctataaa ctgctcaacg ccagcgacgc cactggcggc aacggttcgg atgtggtgaa
    21841 tggctttacg gtaggcacct gggaaggcac tgccgacacc gatcgcattg atatccgcga
    21901 gttgttgcag ggcagcggct acaccggaaa cggcaaagcc agctatgtca acggcgtggc
    21961 gacgctggat gcgcaggccg gaaacatcgg tgactttgtc aaagtcaccc agagcggcag
    22021 cgacaccatc gtgcagatcg accgcgacgg cacgggcggc acttttgcga caactaacgt
    22081 ggtcacgctg acgggcgtgc acaccgacct cgccactttg ctggcgaatc atcagttgat
    22141 ggtggtgtag ataatgaaaa aaaatgccca gtccgtggaa gcctggctgg aggcgatgat
    22201 cgccgtggcg cgctattatc ggctcgattt ttctcaggag aatgtgcgcg cgaccgtcaa
    22261 ctgggagcgc gacagtaagc gggaagagtt gctcaccgac atggcgcggc agttgggaat
    22321 ggggttacgg ctggtggagt tttccgccga ttcgcttaac ccctggcgtc tgccgctgat
    22381 cgcggtgttt gataatcagc agattggcgt gatcacccgt cgcgacaatc atgacaatat
    22441 cagcgttcag ttcagcggtg acgaagggct ggaaaccacg cttaacgtcg cggatgtcga
    22501 ggataaaatc gttgaactgg cgctactgcg cccgctcagc gccatccctg acgcgcgggt
    22561 agatgactat attcgcccgt atcaggcgaa ctggttctgg agtctttcgc taaaagactg
    22621 gcggcgctac ggcgatatta tgctggcgtc actggtggcg aacgtgctgg cgctggcggc
    22681 gatgattttc tcgatgcagg tttacgaccg cgtcgttccg gcgcagtcct acccgacgct
    22741 atgggtgctg tttgccgggg tgatgatggc gatcctgttt gagttctgta tgcgcatggt
    22801 gcgtacgcat ctctcggatg tgatcggcaa gcgggcggat ctgcgtattt cggatcgcgt
    22861 ttttggtcac gcattacggc tgaaaaacaa cgtgcgatcg aaatccaccg gctcgtttat
    22921 ctcgcagatc cgcgaactgg aatcggtgcg ggagctgatt acttccacca ctatcggcgc
    22981 ggtggcggat ctgccgttct tcctgctgtt tgtttttatt ctgtggatga ttggcggctg
    23041 gctggtgctg gttgttttgc tggcgctgcc gttattggtg atccccggcc tgctggtgca
    23101 acgcccgctg gcgcggctgg cgaacgaagg aatgcgcgag tcagcggtac gcaacgctac
    23161 gctggtggaa gccgtgcagt cgattgaaga tattaaactg cttcgcgctg aacagcgttt
    23221 tcagaatcag tggaatcaca ccaacgacgt ggcctcgtcc atcagcatga agcaacgttt
    23281 tctcaccggg ctactgctca cctggaccca ggaagtgcaa tccatcgtct acgtcgtggt
    23341 gctgttggtg ggctgcttta tggtgatgaa cggcgacatg accactggcg cactggtcgg
    23401 gacgacgatc ctggcttcgc gcaccatcgc gccgctgtcg cagatatccg gcgtgctctc
    23461 gcgctggcag caggcgaaag tagcacgtaa cggccttgat gaactgatga agcgccccgt
    23521 ggatcagccg gaacatggca aactggtgca taaagcggtg ctgcacggta attatcagtt
    23581 cagcaacgcg gtgttttatt acgacgaaga agagaaaatt gccgatgtgg cgatcggtaa
    23641 actcaacatt caggccggag agaaaatcgc cattctcggg cgcaacggcg cgggtaaatc
    23701 gacgctactg caaatgctcg ccggaatgcg tatcgcccag caggggcagg tgttgctgga
    23761 taacatcagt atcgggcagc ttgatccggc agatctgcgg cgagacatgg ggctactcag
    23821 ccagaccggg cgactgttct ttggttcgct gcgggaaaac ctcaccatgg ggatgccgga
    23881 agccagcgat gaagatatcg aacgcgcgtt aaccctcagc ggagcgctgc cctttgtgca
    23941 gaagcagaaa aacggcctca actacatgat tcaggaaggg ggatttggtc tttctggcgg
    24001 tcagcgacaa acgctgctgc tggcgcggct actcatctct caacccaata tcgtcctgct
    24061 tgatgaaccc agcgcctcgc tggatgaaat ggcggaagcg tatttaatcg agcaactgaa
    24121 acagtggatt gggcatcgca ctctgatcgt cgctacccac cgcacggcga tgctgcaact
    24181 ggtggatagg ataatcgtga tggatcaggg gcggattgtg atggacggcg cgaaagaggc
    24241 cattttgcgt gaacagggcg aacccacggc gcgacgggtg gtgttgcagg aaaagaataa
    24301 ggggtctgcc gcatgaacgg acaactgcaa tataaccgtg gcctgactga accccgcttg
    24361 ccgcgttcgg cgctggcggt acgcgttacc gctgtgatgc tgctctgctt tcttggctgg
    24421 gcgtggtact tccagcttga tgaagtcacc accggcagcg gcacggtaga accctccggg
    24481 cgcgagcagg tggtgcagtc gctggaaggc ggaattttgt atcatctcga cgtcaaggtt
    24541 ggcgatattg ttgaacaggg ccagccgctg gcgcagctta accgcaccaa aacggaatcc
    24601 gacgtccagg aagcgatgtc gcggctgtac gctgcgctgg caacctcggc gcggctgcgg
    24661 gcggaagtga gcaataaacc gctggtcttc cctgacgaac tgaataaatt ccccgaactt
    24721 atcgagtcgg aaacggcgtt gtacaacact cgccgcgacg ggttgaacaa agccaccacc
    24781 gggctgacgc aggggatcag tctggtcaat cgcgaactgg cgatgaccca gccgctggta
    24841 aaacaggggg cagcgagcag cgtcgaagta ctgcgcctgc aacgccaggc caatgaactg
    24901 gaaaacaaac tcagcgacgt gcgcacgcaa tactacgttc aggcgcggga agagttagcg
    24961 aaagccaatg cggaagtgga aacccagcgt tcggtgatcc gtgggcgtga agactccctc
    25021 accaggctca actttactgc gccagttcgt ggcattgttc aggatattga cgtcaccacg
    25081 gttggcggcg tcatcgcccc cggtggtaaa ctgatgacca tcgtgccgct ggatgagcaa
    25141 ctgctgattg aggcaaaaat ctccccgcgc gatgtggcgt ttatccatcc ggggcaaaaa
    25201 tcgctggtga aaataactgc ctacgattac tccatctacg gcggtctgcc gggagaagta
    25261 gcggtgatat caccagacac cgtgcaggat gaagtgcggc gcgatgttta ttactatcgg
    25321 gtgtacattc gcactttcag caaccatctg gaaaacaaaa gcaaacagca gtttccgata
    25381 ttccccggta tggtggcgac ggtcgatatc cgcaccggca aaaaaagcgt gctggattat
    25441 ctgttgaaac cctttaacaa agcacaggag gcgttgcgcg aacgatgaac attagcgatg
    25501 tagcaaaaat taccggcctg accagcaaag ccattcgctt ctatgaagag aaggggctgg
    25561 tgacgccgcc gatgcgcagc gaaaatggtt atcgaaccta cacgcagcag catctcaacg
    25621 aattgacctt actgcgccag gcgcgccagg tgggctttaa cctggaagag agcggcgagc
    25681 tggtgaatct gtttaacgac ccgcagcgtc acagtgccga cgtcaaacgt cgcacgctgg
    25741 agaaggtggc ggagattgaa cggcatatcg aagaactgca atctatgcgc aaccagctac
    25801 tggcgctggc gaatgcctgc cccggtgatg acagcgccga ctgcccgatt atcgaaaatc
    25861 tctccggctg ctgtcatcat cgggcggggt gaaaatcagt taattcccgt ttgcaccgaa
    25921 agaatatgct gcaaactttc taccataatg cgccccgact gaaataagcc ttcctggatt
    25981 tcgttgttaa cattcacagc gtgaaccagg gaggctaata atactacttg caagaaggca
    26041 aaaataaacg caccgataat cataatatgc gtcaggggta ttttcctttg ctccttcatt
    26101 ttaaataacc cacaaactgg ccttctttca ttaaggcgac ttttcctgtg tgatgataaa
    26161 caatgacgtt atacagacct gactgataaa cagtggcatc tttattgtct tcatcaattg
    26221 cacataatga atcattgaga taagcaaagt ttttactgct cattctaaag gtcatgccaa
    26281 atcctttata aatggctttc tttgccgcct gggcatgttg tgcggcttct gcatttttaa
    26341 atgcctgcgc ttcggaagca cttaaaatag ccgtctggcg ctgctgttct gcgagataac
    26401 aggtgtcatg gctgacgccc tgcgcctggc aatctgcgat gcgggaagcg ggtgagctac
    26461 agccagccag taatataacc gccagcaggg aaaacgattt aatggagcta ttcatcattg
    26521 tta
//
LOCUS       DAZKFU020000041        26164 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZKFU020000041 DAZKFU020000000
VERSION     DAZKFU020000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.041"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..317
                     /gene="bhsA"
                     /locus_tag="ACHOQQ_RS06795"
                     /old_locus_tag="pgaptmp_001359"
                     /gene_synonym="ycfR"
     CDS             60..317
                     /gene="bhsA"
                     /locus_tag="ACHOQQ_RS06795"
                     /old_locus_tag="pgaptmp_001359"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGU4469632.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(399..1361)
                     /gene="ldtC"
                     /locus_tag="ACHOQQ_RS06800"
                     /old_locus_tag="pgaptmp_001360"
     CDS             complement(399..1361)
                     /gene="ldtC"
                     /locus_tag="ACHOQQ_RS06800"
                     /old_locus_tag="pgaptmp_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGU4469633.1"
                     /translation="MMIKTRFSRWLTFFTFATAVALALPVKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1505..4951)
                     /gene="mfd"
                     /locus_tag="ACHOQQ_RS06805"
                     /old_locus_tag="pgaptmp_001361"
     CDS             complement(1505..4951)
                     /gene="mfd"
                     /locus_tag="ACHOQQ_RS06805"
                     /old_locus_tag="pgaptmp_001361"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGU4469634.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEVINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQNDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREVLRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5079..6152)
                     /locus_tag="ACHOQQ_RS06810"
                     /old_locus_tag="pgaptmp_001362"
     CDS             complement(5079..6152)
                     /locus_tag="ACHOQQ_RS06810"
                     /old_locus_tag="pgaptmp_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU4469635.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVLQW
                     LALSALNQWLAPERDLSNASNAAYADSTGGFLHGMITASTSLWYLYALIVYFVLCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6414..7613
                     /gene="lolC"
                     /locus_tag="ACHOQQ_RS06815"
                     /old_locus_tag="pgaptmp_001363"
     CDS             6414..7613
                     /gene="lolC"
                     /locus_tag="ACHOQQ_RS06815"
                     /old_locus_tag="pgaptmp_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGU4469636.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRFPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWHDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7606..8307
                     /gene="lolD"
                     /locus_tag="ACHOQQ_RS06820"
                     /old_locus_tag="pgaptmp_001364"
     CDS             7606..8307
                     /gene="lolD"
                     /locus_tag="ACHOQQ_RS06820"
                     /old_locus_tag="pgaptmp_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGU4469637.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8307..9551
                     /gene="lolE"
                     /locus_tag="ACHOQQ_RS06825"
                     /old_locus_tag="pgaptmp_001365"
     CDS             8307..9551
                     /gene="lolE"
                     /locus_tag="ACHOQQ_RS06825"
                     /old_locus_tag="pgaptmp_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGU4469638.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9580..10491
                     /gene="nagK"
                     /locus_tag="ACHOQQ_RS06830"
                     /old_locus_tag="pgaptmp_001366"
     CDS             9580..10491
                     /gene="nagK"
                     /locus_tag="ACHOQQ_RS06830"
                     /old_locus_tag="pgaptmp_001366"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGU4469639.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10507..11328
                     /gene="cobB"
                     /locus_tag="ACHOQQ_RS06835"
                     /old_locus_tag="pgaptmp_001367"
     CDS             10507..11328
                     /gene="cobB"
                     /locus_tag="ACHOQQ_RS06835"
                     /old_locus_tag="pgaptmp_001367"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGU4469640.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11484..12530)
                     /gene="potD"
                     /locus_tag="ACHOQQ_RS06840"
                     /old_locus_tag="pgaptmp_001368"
     CDS             complement(11484..12530)
                     /gene="potD"
                     /locus_tag="ACHOQQ_RS06840"
                     /old_locus_tag="pgaptmp_001368"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGU4469641.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(12527..13321)
                     /gene="potC"
                     /locus_tag="ACHOQQ_RS06845"
                     /old_locus_tag="pgaptmp_001369"
     CDS             complement(12527..13321)
                     /gene="potC"
                     /locus_tag="ACHOQQ_RS06845"
                     /old_locus_tag="pgaptmp_001369"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGU4469642.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(13318..14175)
                     /gene="potB"
                     /locus_tag="ACHOQQ_RS06850"
                     /old_locus_tag="pgaptmp_001370"
     CDS             complement(13318..14175)
                     /gene="potB"
                     /locus_tag="ACHOQQ_RS06850"
                     /old_locus_tag="pgaptmp_001370"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGU4469643.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(14159..15295)
                     /gene="potA"
                     /locus_tag="ACHOQQ_RS06855"
                     /old_locus_tag="pgaptmp_001371"
     CDS             complement(14159..15295)
                     /gene="potA"
                     /locus_tag="ACHOQQ_RS06855"
                     /old_locus_tag="pgaptmp_001371"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGU4469644.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15545..16771
                     /gene="pepT"
                     /locus_tag="ACHOQQ_RS06860"
                     /old_locus_tag="pgaptmp_001372"
     CDS             15545..16771
                     /gene="pepT"
                     /locus_tag="ACHOQQ_RS06860"
                     /old_locus_tag="pgaptmp_001372"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGU4469645.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(16820..17941)
                     /gene="roxA"
                     /locus_tag="ACHOQQ_RS06865"
                     /old_locus_tag="pgaptmp_001373"
                     /gene_synonym="ycfD"
     CDS             complement(16820..17941)
                     /gene="roxA"
                     /locus_tag="ACHOQQ_RS06865"
                     /old_locus_tag="pgaptmp_001373"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGU4469646.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(18017..19477)
                     /gene="phoQ"
                     /locus_tag="ACHOQQ_RS06870"
                     /old_locus_tag="pgaptmp_001374"
     CDS             complement(18017..19477)
                     /gene="phoQ"
                     /locus_tag="ACHOQQ_RS06870"
                     /old_locus_tag="pgaptmp_001374"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGU4469647.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(19477..20148)
                     /gene="phoP"
                     /locus_tag="ACHOQQ_RS06875"
                     /old_locus_tag="pgaptmp_001375"
     CDS             complement(19477..20148)
                     /gene="phoP"
                     /locus_tag="ACHOQQ_RS06875"
                     /old_locus_tag="pgaptmp_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGU4469648.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(20316..21686)
                     /gene="purB"
                     /locus_tag="ACHOQQ_RS06880"
                     /old_locus_tag="pgaptmp_001376"
     CDS             complement(20316..21686)
                     /gene="purB"
                     /locus_tag="ACHOQQ_RS06880"
                     /old_locus_tag="pgaptmp_001376"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGU4469649.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(21690..22331)
                     /gene="hflD"
                     /locus_tag="ACHOQQ_RS06885"
                     /old_locus_tag="pgaptmp_001377"
     CDS             complement(21690..22331)
                     /gene="hflD"
                     /locus_tag="ACHOQQ_RS06885"
                     /old_locus_tag="pgaptmp_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGU4469650.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(22367..23473)
                     /gene="mnmA"
                     /locus_tag="ACHOQQ_RS06890"
                     /old_locus_tag="pgaptmp_001378"
                     /gene_synonym="trmU"
     CDS             complement(22367..23473)
                     /gene="mnmA"
                     /locus_tag="ACHOQQ_RS06890"
                     /old_locus_tag="pgaptmp_001378"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGU4469651.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRVDVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(23527..23988)
                     /gene="nudJ"
                     /locus_tag="ACHOQQ_RS06895"
                     /old_locus_tag="pgaptmp_001379"
     CDS             complement(23527..23988)
                     /gene="nudJ"
                     /locus_tag="ACHOQQ_RS06895"
                     /old_locus_tag="pgaptmp_001379"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGU4469652.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(23998..24651)
                     /gene="rluE"
                     /locus_tag="ACHOQQ_RS06900"
                     /old_locus_tag="pgaptmp_001380"
     CDS             complement(23998..24651)
                     /gene="rluE"
                     /locus_tag="ACHOQQ_RS06900"
                     /old_locus_tag="pgaptmp_001380"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGU4469653.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            24823..26073
                     /gene="icd"
                     /locus_tag="ACHOQQ_RS06905"
                     /old_locus_tag="pgaptmp_001381"
     CDS             24823..26073
                     /gene="icd"
                     /locus_tag="ACHOQQ_RS06905"
                     /old_locus_tag="pgaptmp_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGU4469654.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         6320 a         6672 c         6855 g         6317 t
ORIGIN      
        1 tagttatcga tcgttaagta attgcttgcg acgtcattca tctgcataag gccactatta
       61 tgaaaaacgt aaaaactctc atcgctgcgg cgattttaag ctccatgtca tttgccagct
      121 ttgcggctgt cgaagttcag tcaacgccag aaggccaaca aaaagtcggt acaatcagtg
      181 ctaacgcggg gacaaatctg ggatcgctgg aagagcagct ggcgcaaaaa gcggatgaga
      241 tgggcgcaaa atctttccgt attacttctg taaccggtcc gaataccctc catggaacag
      301 cagtaattta taaataagca ttaaccctca ttaatgcctg ctactgctga ttttttcccc
      361 gcgacatgcc gtgtcgcggg gattttttta tccaggattt acagagtttg tgggctcact
      421 tgatgacggc ggacatccac cggcatcccg gaacggacat ccatcacatg ttgcatcact
      481 tcagcgtcag tttgtgctgc atctttaaat gattgcattg cgctattcag cgtaattggc
      541 agcagctgcg gatcgtcatc aatcttctct gacagcggct gatgtacttc aaccagacgc
      601 gcaccgtttg gttcggcaga gacttttatc ggagtgttga tgatattcac tttggtgcct
      661 ggggtgacct ggctaaagag tgttttgata tcgtcatccc gcagacgaat acagccagaa
      721 cttacccgca tgccaatgcc gaaatcggcg ttcgtaccgt gaagcaaata aacgccgcca
      781 taggccgcca gacgaatcgc atgatggccc attgggttat ccggtccagc cggcactacc
      841 gcaggcaact caattccctg tgccttatac cgcgcgcgga tgtttgccgt tggcgtccag
      901 gttgggtttg ctcgtttgtc tgaaacggtg gtcaccattg tcggtgtcag cgtgtcacca
      961 cccaactgac caatacctat tggatacacg gttaccgaat ttttacccgg cgggtagtaa
     1021 tagagacgca gctccgcaat attgatcaca atgccttcgc gcggcgcatc tggaagtagg
     1081 gtttgcaacg ggatcgttaa cacgctgccc gcgcgcggta cgtaaggatc aacgccgggg
     1141 ttagcctgta acagagcgag aaagccgacg ttatattttt tggcgatggc ttccagagaa
     1201 ccaccgtcat tttccaccac atgaaatttg ttttcgccaa ccagacgact gcccgctggc
     1261 ggcagcggcc aggtgttggc ttttaccggt agcgccagcg ccacggcagt ggcgaacgta
     1321 aaaaacgtta gccagcgaga aaaacgcgtt ttgatcatca ccaaaaatcc ataataaata
     1381 taaggttatt gtaataaaca gataagcctg aattatggat ggtgacagtg tcggatagtg
     1441 cagggaagtg caaagaattt gtaaatgttg cagatggggg cgcagaaacg cccccgattt
     1501 accattaagc gatcgcgttc tcttccagtt cacgcataaa ctggcgtacc cattcgatac
     1561 gcgttttccg ctcactcaaa tcctgaataa atttcaggcg cgtcggacca tcaaggcggt
     1621 aatgctgcgg ctgtttttgc agcaaaccaa tcaaccaggc cggattaacg tgattcttct
     1681 cggcaaattc gatcacgccg cctttctcat taccttccag cttcctgatc cccagtttct
     1741 gcgcttgctg gcgcagacgg gcaatatcca gcagggtacg cgccggatcc ggcagcaggc
     1801 cgaagcgatc gataagctcg actttgatct cttccagttc gttttccgtt ttggcgctgg
     1861 caatacgttt gtagaacgac agacgcgtgt tcacgtcagg gatgaaatca tctggcaata
     1921 gcgacggcat ccgcagctcg acttctgttt gctggctggt gagatcttcc agcgacggct
     1981 cgcgtccggc tttcagtgca tcgacggcgt tttccagcaa ctccatatac agcgagaaac
     2041 cgatggtttc cattgagccg ctttgttctt cgccaagcag ttcacccgcg ccgcggatct
     2101 ccagatcgtg cgttgccagc gcaaaacctg ccccgagatc ttccagcgag gcaatcgctt
     2161 caagacgttt ttgcgcatcg gtagtcatcg cttttggatg cggcgtcagc agccatgcat
     2221 atgcctgatg atgtgaacgt ccgacgcgac cgcgcaactg gtgcagctgc gccagaccga
     2281 agtgatccgc gcgttcaatg ataatggtgt tggcggtagg gatatcgatt ccggtttcga
     2341 taatggtggt acaaaccagc acgttgaaac gttgatgatg gaaatcattc atcacccgtt
     2401 ccagttcgcg ctcgcgcatc tgcccgtgac cgatggcaat ccgtgcttct ggcaccagtt
     2461 ctgccagccg ttcggcagct ttctggatgt tttccacatc attgtagaga taataaacct
     2521 gaccgccgcg cagaacttca cgcaggatcg cctcccggac caccagactg tcatactcgc
     2581 ggacaaacgt tttgaccgcc agacgacggg cgggcggcgt ggcgataatc gacaggtcac
     2641 gcattccgct catcgccata ttcagcgtac gtgggatcgg cgtagcggta agcgtcagaa
     2701 tatccacgtt cgcacgcatc gctttaatgc gctctttatg acgcaccccg aagcggtgtt
     2761 cttcatcgac aatcagcaga cctaaatctt taaacttgac gtcattttgc agcagtttgt
     2821 gcgtaccgat cagaatatcg attttccctt ccgccacttc cgcaaggatt tgcgtttgct
     2881 ctttggcgct gcggaaacgg gagatcattt cgatacgtac cggccagttg gcgaaacggt
     2941 cgcggaagtt gtcgtaatgc tgctgcgcga gaagggtggt aggcaccagc accgctacct
     3001 gcttgtggtt atctactgcc aggaaagcgg cgcgcatcgc cacttctgtt ttcccaaagc
     3061 caacatcgcc gcacaccaga cgatccattg ccagcggctg acacatgtcg ctaagcaccg
     3121 cattaatggc ctgcgcctga tccggcgtgg tttcaaacgg gaagctgtcg cagaacaact
     3181 gatactgctc acgatcgtgt ttaaacgcga agccctcttt ggcggcgcgt tgcgcgtaga
     3241 tatccagcaa ttctgccgcc acatcacgca ctttttccgc cgctttctgc cgcgcgcgtg
     3301 accacgcatc gccgccaagt ttatgcagtg gggcgttttc ttccgcgcca cctgcgtaac
     3361 ggctaatcag atgcagtgac gacaccggaa catacaattt ggcgtcgttg gcataggtga
     3421 gcatcaggta ctcgccggta atgccgccag cttccagcgt ggtcattcct gcatagcgcc
     3481 cgacgccgtg ctccagatgg accaccggct gaccaatgtg caactccgcg aggttacgga
     3541 tcagtgtatc ggggttgatg gtgcggcgag agtcctgacg acggcgggca acgcgttcac
     3601 cgagcagatc gctttcgcaa atcagcgcca ggttacgcac cttatcgaca aaaccatgtt
     3661 cggcagcgcc aatcatcaaa taacgcccac ggtcgctggc ttcatcaaga cgcataatgc
     3721 gttgcggagc aattttaatt cgcgcgagca gttcacccag cgcttcacgg cgaccttcac
     3781 tttctaccga gaacaccacc ggaccgtcga aagactcgag gaacttacgc agcgcatcca
     3841 gcggcgcttt ttgttgtgcc tgaacggcca ggtctggcag tttctggaaa cctaaattgg
     3901 cattcgcggc ttttgtcggt aaatgttcag tttttagctg cactcggggc cagtttttca
     3961 gctctgagaa gagctcgtcc acccgcagcc agagcgattg tggtggcaac agtgggcgca
     4021 tcggatcgac gccgcgattc tcaaaacgcg ccagcgtgtc agcctggaaa cgttcggcac
     4081 tgttttccag atcgccagta ttcaccagca aggtattggc agggaaataa ctgaacagcg
     4141 gcggcagtgg ttcgctgaag aacaatggct gccagtactc gatcccggca ggtaatgtgc
     4201 ctttactcac ttgctggtaa atatgttccg gatcgcgctt cacttcgaag gtatcgcgcc
     4261 actggctgcg gaacagttca attgccgctt tatcggtcgg aaattcgtgc gcgggcagca
     4321 gattgatcac ttctacttcc tccagcgtgc gctggctgtc gacgtcaaac acccgcaggc
     4381 tgtcgatttc atcatcaaag aaatcaagac gataaggcag ctcactcccc atcgggaaga
     4441 gatccagcaa cgcgccgcgc gtggcgtatt cgccgtgctc catcacctgg tcaacatggc
     4501 gataaccggc gctgtccagt tgggttcgta atgcatctcg tgacaggcgc tgaccttttt
     4561 tcatcaccag cgcatgaccg tggagaaaac tgtgtgggca aacgcgctgc ataagcgtat
     4621 tcaccggaac aatcagtacg ccacgctgca tcgtcggtag ctggtaaagg gtggaaaggc
     4681 gcgaggagat aatgtcctga tgaggcgaaa aactgtcgta agggagggtt tcccagtccg
     4741 ccagattcat caccatctga tcggtgaact ggctgatttc atcatgcaaa cgcagagcat
     4801 tttgcatatc tggtgcaatg agtaccaccg gaccggcgtg acgttcggca atttccgcca
     4861 ccagcgtcgc gcaggcagcg ccggttaact cgcccagcag acgctgctca cccgctttga
     4921 cgggcagcgt ataacgatat tgttcaggca taacggttgt cagattctcg ttaggatatg
     4981 cctcaacata tgggggcata tctctgataa gcaatgctgt tcattatccg taatcgacat
     5041 gctttagcaa atcgaatcgc cccggcgggg gcgatggctt agcgggagta actgacggca
     5101 gcgggcagtg aaggcgggga gaacaacaga tcgctgaatg cacgctgtga aagttttctt
     5161 accagcaagc ccgcaacagt acagatgaac aaactgacaa agggataaac cagcaggagt
     5221 gtcagttcga cttgcggcga ccagcgtgct gcgttcattt gcgcaagcag agttaagctg
     5281 aatatttcaa ccagaatgcg atgggtggtg tagatagcaa tggtgttgga gccaatcaca
     5341 ttcagcaggc tggtggagcg cataccgaat cgttgctcgt attgataaaa cagcttcatg
     5401 atcaccacaa tcgataccag cgacaacagc agcgagatag taaacaacca ggcaccgacc
     5461 gccagaacgg tcagcaaaga agccatcagc agatggcggc gcaacggcac ctctttaaca
     5521 caggtcatta ttgttgcgcc aaaccatgcg ccaaggctgt aataaggcaa attgcggatc
     5581 acactgttca ttccccacca cggcgtggga acgaaattaa ccgccacact cagcagtaca
     5641 aacaaggcga atagtggcag cgccagacgg ctaaaaattt tacataacac gaaatagaca
     5701 attaacgcat acagatacca caagctggtg ctggcggtga tcatcccgtg caggaaccca
     5761 ccggtggaat cggcataagc ggcattggag gcattgctta aatcgcgctc aggtgccagc
     5821 cactggttca gcgcacttaa cgccagccac tgcagcacgc cccaaagcgc cagcacccag
     5881 aagatgttcc agatccgttt atcgagacaa tttccccacg gtacgctgtc gatatagcgg
     5941 cgaatcaaat agccggaaat aaagaaaaaa accggcatac gaaagggggc aaggtaaaga
     6001 ttgaaataga tccagcattt gctcaggact tccgataacg gatgctgaaa agtggtcaga
     6061 tgcggataaa aggtaatgac cgagtgataa atcacgacca gacaaataca taaccctttg
     6121 atctggttaa tccatagctc tttttgtttc atcaggacaa cacgctcttt tttaatttac
     6181 atgcggttga tcctgcgggt tatgacccga ggtgtaatgg gtatctgtct gtattggatg
     6241 atttttcaga ttaagatcag gcggcaagat tgatgataaa acatggcaat ttagccgatt
     6301 gatttacggg ggcttttcag attagccctg acgatcactt acagttcaga cgtttaccca
     6361 tcctgctttc gcttatatac tcgtgtcttt gctacagcaa ccagacggat ttcatgtacc
     6421 aacctgtcgc tctatttatt ggcctgcgtt acatgcgtgg gcgtgcagcg gatcgcttcg
     6481 gtcgtttcgt ctcctggctt tctaccatcg gcattaccct cggggtgatg gcgctggtca
     6541 cagtattgtc agtgatgaac ggctttgagc gcgagctgca aaacaacatc cttggcctga
     6601 tgccacaggc aattctttct tctgagcatg gctctcttaa cccgcagcaa ctcccggaaa
     6661 cggcagtcaa actggacggc gttaatcgcg ttgcacctat tactaccggt gatgtggtac
     6721 tgcaaagcgc gcgcagcgtg gcggtcgggg tgatgctggg tatcgacccg gcgcaaaaag
     6781 atccactaac gccgtatctg gtcaatgtga aacaaactga cctcgagccg gggaaatata
     6841 atgttatcct cggtgaacag cttgcctcac agttgggcgt caatcgcggc gatcaaatcc
     6901 gcgtgatggt gccatctgcc agccagttca cgccaatggg gcgtattcca agccagcgcc
     6961 tgttcaatgt gattggcact ttcgccgcca acagtgaagt cgatggctat gaaatgctgg
     7021 tgaatattga ggatgcctcg cgtctgatgc gttttccggc aggcaatatt accggctggc
     7081 gtttgtggct ggatgagccg ctgaaagttg actctttaag tcagcaaaaa ctgcctgaag
     7141 gcagcaaatg gcatgactgg cgtgaccgta aaggcgagct gttccaggcc gtacgcatgg
     7201 aaaaaaatat gatgggctta ctgctgagcc tgattgtcgc cgttgcggcg tttaacatta
     7261 ttacctcgct ggggctgatg gtgatggaga agcagggcga agtagcgatc ctgcaaacgc
     7321 aaggcttaac tccgcgacaa atcatgatgg tctttatggt gcaaggggcc agcgccggga
     7381 ttatcggtgc gatcctcgga gcggcgcttg gcgcactgct tgccagccag ttaaataatc
     7441 tgatgccgat aatcggcgtc ctgcttgatg gcgcggcgct gccggtggct atcgaacctt
     7501 tacaggtcat tgttattgcg ctggtggcga tggctatcgc gctgctgtct acgctttacc
     7561 cttcatggcg cgctgccgcc acacaacccg ctgaggcttt acgttatgaa taagatcctg
     7621 ttgcaatgcg acaacctgtg caaacgctat caggaaggca gtgtgcaaac cgatgtgctg
     7681 cacaatgtca gtttcagcgt gggcgaaggt gaaatgatgg caattgtcgg tagctctggt
     7741 tccggtaaaa gtaccttgct gcacctgctg ggcgggctgg atacaccaac ctccggcgat
     7801 gtgatcttta acggtcaacc gatgagcaaa ctgtcttcgg cggcgaaagc agagctgcgc
     7861 aaccagaagc tgggctttat ttatcagttt caccacctgc tgccggattt taccgccctg
     7921 gaaaacgtgg ctatgccgct gctgattggc aagaaaaagc ccgctgaaat taacagccgt
     7981 gcgctggaga tgttaaaagc ggtggggctg gagcatcgtg cgaatcaccg cccatctgaa
     8041 ctttctggcg gcgaacgcca gcgtgtggcg attgcccgtg cgctggtcaa taacccgcgt
     8101 ctggtactgg cggatgaacc taccggtaac ctcgatgcgc gtaacgccga cagcatcttc
     8161 cagttgctcg gagaattgaa tcggttgcag ggcactgcct tcctggtggt tactcacgac
     8221 ctgcaactgg cgaaacgtat gagccgccag ttagaaatgc gtgatggtcg tttgacggca
     8281 gaactaagcc tgatgggggc ggagtaatgg cgatgccttt atcgttatta attggcctgc
     8341 gttttagtcg cggacggcga cgcggcggca tggtgtcgct gatctccgtt atttctacca
     8401 ttggcattgc cctcggcgtg gcagtattga tcgtcggctt aagcgcgatg aacggctttg
     8461 aacgcgaact gaataaccgc attctggcgg tggtgccgca tggcgaaata gaggcggtgg
     8521 atcaaccgtg gactaactgg caggaagcac tggataacgt gcaaaaagtg ccaggtattg
     8581 ccgccgctgc gccgtatatc aatttcaccg ggctggtgga aagtggcgcg aatctgcgcg
     8641 caatccaggt gaagggcgtt aacccgcaac aggaacagcg tctgagcgca ttaccctcgt
     8701 ttgttcaggg ggatgcctgg cgcaatttta aagcgggcga acagcaaatt atcatcggca
     8761 aaggcgtggc ggatgcgctg aaagtgaagc agggcgattg ggtgtcgatt atgatcccca
     8821 actcgaatcc cgagcataaa ctgatgcagc caaaacgtgt gcgtttgcac gttgccggta
     8881 ttttgcagtt gagtggtcaa ctcgatcaca gttttgccat gatcccgctg gcggatgccc
     8941 aacaatatct tgatatgggt tccagcgtgt caggtattgc ccttaaaatg acggatgttt
     9001 tcaacgccaa taagctggta cgcgatgcgg gtgaagtgac caacagctat gtttatatta
     9061 aaagttggat tggtacttac ggctatatgt atcgcgatat ccagatgatc cgcgccatta
     9121 tgtatctggc gatggtactg gtgattggcg tggcctgttt caacatcgtc tccaccttag
     9181 tgatggcggt gaaagacaag agtggcgata tcgcagtatt aagaacgctg ggggcgaaag
     9241 atggtttaat tcgcgccatc tttgtctggt atggattgct ggcagggctg ttcggcagcc
     9301 tgtgtggggt gattatcggc gtggtggttt cactgcaact taccccgatt attgagcgga
     9361 ttgaaaagct gatcggtcat cagttcctct ccagcgatat ctattttatt gacttcctgc
     9421 catcggaatt gcactggctg gacgtcttct acgtactggt cacagcattg ttgctgagtc
     9481 ttttggcaag ttggtatccg gcgcggcgcg ccagtaatat tgaccctgcg cgagtcctta
     9541 gcggccagta aaggcagtac attaaaacaa ggagcggcaa tgtattacgg gtttgatatt
     9601 ggtggaacaa aaattgcgct tggcgtgttt gatagcggtc ggcagttgca gtgggaaaag
     9661 cgggtgccga caccgcgtga cagctatgac gcatttttag atgcagtgtg tgagctggta
     9721 gctgaagctg accaacgttt tggctgtaaa ggttctgtcg gcatcggtat tccgggaatg
     9781 ccggaaacag aagatggtac gctgtatgcc gccaatgtcc ccgctgccag cggtaaaccg
     9841 ctgcgtgccg acctgagcgc acgtcttgat cgcgatgtcc gccttgataa cgatgctaac
     9901 tgttttgccc tttcagaagc ctgggatgat gaatttactc aatatccact ggtgatgggg
     9961 ttgattctcg gcaccggcgt tggcggcggg ctgattttca acggtaaacc gattaccggt
    10021 aaaagctata ttaccggcga gtttggccat atgcgtctgc cggttgatgc gttaaccatg
    10081 atggggttgg atttcccgtt acgccgctgc ggctgtggtc agcatggctg cattgaaaat
    10141 tatctgtctg gtcgcggttt tgcgtggctg tatcaacact attatcatca accgttgcag
    10201 gctcccgaaa ttattgcgct ttatgatcaa ggcgatgagc aggcaagggc gcacgttgag
    10261 cgttatctgg atttattagc ggtttgtctg ggaaatatcc tgaccattgt tgaccctgac
    10321 ctggtcgtca ttggtggtgg cttatcgaat ttcccggcaa tcacaacgca actggcggac
    10381 aggctgcctc gtcatctctt acctgtagct cgtgttccgc gcattgaacg cgcgcgccac
    10441 ggtgacgcgg gaggaatgcg tggtgcggcc ttcctacatc taaccgatta aacaacagag
    10501 gttgctatgc tgtcgcgtcg gggtcatcgg ttaagtcgtt ttcgtaaaaa taaacgccgc
    10561 ctgcgcgagc gtttgcgtca gcgtattttt ttcagagata aagtggtgcc ggaagcaatg
    10621 gaaaaaccaa gagtactcgt actgacaggg gcagggattt ctgcggaatc aggtattcgt
    10681 acctttcgcg ccgcagatgg cctgtgggaa gaacatcggg ttgaagatgt ggcaacgccg
    10741 gaaggtttcg atcgcgatcc tgaactggtg caagcgtttt ataacgcccg tcgtcgacag
    10801 ctacagcagc cagaaattca gcctaacgcc gcgcatcttg cgctggctaa actgcaagat
    10861 gctctcggcg atcgcttttt gctggtgacg cagaatatag acaacctgca tgaacgcgca
    10921 ggtaatacca atgtgattca tatgcatggg gaactgctga aagttcgttg ttcacaaagt
    10981 ggtcaggttc tcgactggac aggagacgtt accccagaag ataaatgcca ttgctgccag
    11041 ttcccggcac ccttgcgccc gcacgtagtg tggtttggcg aaatgccact cggcatggat
    11101 gaaatttata tggcgttgtc gatggccgat attttcattg ccattggtac ttcggggcat
    11161 gtttatccgg cggctgggtt tgttcacgaa gcgaaactgc atggcgcgca caccgtggaa
    11221 ctgaatcttg aaccgagtca ggttggtaat gaatttgccg agaaatatta cggcccggca
    11281 agccaggtgg tgccagaatt tgttgaaaag ttgctgaagg gattataaag caggaagcat
    11341 tgcctgatgc gctacgctta tcaggcctac aagcggacca caataccgac gaaagagcgg
    11401 gataaaactt tactgtcctg actcattagc cacatccttg ctaactaaaa aacgggcggt
    11461 aataccaccg cccgcttgct gaattaacgt cctgctttca gcttctgata atactcttca
    11521 taaatgctgc tggctgcgcc aacgtcattc tgccattcgc catttttaat ggtttcagca
    11581 tccgggtaga gcgtcttatc gttcgccact tctggactta acagcttacg cgccgcaagg
    11641 tttggcgttg gataaccgat agtttcagca acctgttttg ccacatccgg gcgcagcagg
    11701 aagttgatca atttcagcgc gccttctttg tttttggcat ttgccgggat cgccaggctg
    11761 tccatccaga atatgccgcc ttctttcggc cacaccacgt caataggcgt acccgcctgg
    11821 cgtgcaacga aagcagaacc gttccagatc atgccgaggt taacttcgcc ttccatgtac
    11881 gggttagccg ggttatcgga gttaaacgct gcgacgtttg gcatcagttt tttcagctcg
    11941 ttatatgcag cttcaatctc tttcggatcg gtggtgttac cggagtagcc cagcttacgc
    12001 agcgccatct ggaacacttc acgggcatcg tcggtcagca gcaggctgcc tttgtactca
    12061 ggcttccaca gatcggccca gctggtaacg gatttcggat ctaccgcatc accgttaacg
    12121 ccaatcgccg tcgcccccca gatatacgga atggagtagt cgttattcgg gtcaaacggc
    12181 ttgttgagca tgtctggatc gaggttgctg aaattgctta acttcgactt gtcgatcttc
    12241 tggatcatcc cttctttacg cattttatcg acgtaatagg ttgaaggaac caccaggtcg
    12301 taggcaccgt ctttgtacgt tttcagcttg gcgtacatgg tttcgttcga ctcgtaagtc
    12361 gaatagataa ccttaatacc ggtttctttg gtgaactgtt caagcagtcc tggcggcacg
    12421 tactcggtcc agttgtagaa atacagcgtg ttgttgtcat cggcgtgagc ggcgctcatg
    12481 cccagtgcca gagcacccgc cgcgagcagg tggcgtgacc attttttcat ttaacgtccc
    12541 ctgtgttacc tttcgtttta tcacgagcaa taagctggct ggcaataacc atcaccagcg
    12601 acagcaccag taatatggtt gccagcgcgt taacttccgg cgatacgcca actttgacca
    12661 tcgaataaat ttttaacggc agaatttcat aacttggtcc ggtgacgaac gaagaaacta
    12721 ccacatcgtc catcgacagg gtaaagctta acacccagcc cgccgccacc gctggcattg
    12781 ccagtggcag aatgattttt cgcagaatgg taaattcgct ggcaccgaga tctttcgccg
    12841 cttccagcat tcgcacgtca aaacctttca ggcgcgaata caccgtcacc accacaaatg
    12901 gcaggcagaa ggtgatatgc gagaacagca gcgaccagaa gccaagctga atacccagca
    12961 gcataaacag caccagcagc gaaatcgcca tcacgatatc tggtgacatc atcaccacaa
    13021 acagcattcc gctaacgaac ggcttaccac gaaagcgata acggtacagc gcaactgccg
    13081 tcagtgaacc gataagcgta gcaaacgtcg ccgaaaacac cgccattgtc agtgaatgct
    13141 gcgctgcctg taacaggctg tcgttgttca tcagcaagct ataccattta gtggtaaaac
    13201 cctgccagtt gatgccaaag cgcgagctgt taaaggagtt cacaatcaaa ataatgattg
    13261 ggatatacag gtacgcgtag atagcggtca taaaaccgcc gcgaagcagt cgaccgatca
    13321 ttcgagttcc accttcttat tcagcagacg agaagcgcgc cagtaaacca gcaacatcag
    13381 gcccattacg atagtcagcg taatgctggt ggctgcacca aacggccagt cacgaatatt
    13441 aaggaactgg accttgatga cgttaccgat cagcaggttt ttcgcaccgc ccatcaggtc
    13501 ggacacatag aacaggccca tcgctggcag catcaccagc agacatccgg caataatccc
    13561 cggcatcgtc agtggaataa tgatacggat aaaagtctgt aacttgctgg caccgagatc
    13621 gcgcgccgcc tcaagcaacg gcttatccag tttttcgata ctggagtaca gcggcatcac
    13681 cataaacggc agcagaatgt aaaccagacc gataatcacc gcactgggcg tgaacatgat
    13741 acggattggt gtgtcgataa cgcccagcca gagcaaaaac tcgttgaggt agcctttggt
    13801 gctgaggaaa attttcagcc cgtagatgcg aatcagtgag ttggtccaga agggaacaat
    13861 cagcagaaac agcagcagcg gacgcacctt gtgtggcagc ttcgccagaa accaggcaaa
    13921 cggatagccc agcaccaggc aggcgagggt ggcgatcagc gccatattca gcgagtgcaa
    13981 tagcacttca aaatagagcg gatcgagcag acgtgtgtag ttatccagcg taaagaccat
    14041 tttgacgaaa ctggcgtcgt cgcgggtcaa aaagctggtg ccaatgatca tcaggttggg
    14101 cagaaagaca aacaacacaa gccaaccgac aatagtgaca atcactacat tctggaactt
    14161 acttgtgttc ttcatcagcc agtacgacct cccagctttc tacccaatta atggccattt
    14221 tttggtcgag agagtggtca aagtcaggat cgtcttcatt gaagaattcg ctgaccatca
    14281 ccatcttgcc attttccagt tcaacaaccg actccagcgt catgcctttg tagttacgct
    14341 cgcgaacgta accaatcagc ccttcagcgt ggttgtcgtc gttaatctct tcaacacgta
    14401 agtcttccgg gcgcagcaga acatgcagtt tttgccccgg ttcaacggcg aagttaacgt
    14461 agatattaca ttcgcggcct tcaacgttgg cgcgtacgcg ctgctcgtcg agacgttcga
    14521 tgacagtggc gttaaacata ttgatttcgc caatgaaccc ggcaacaaac aggtttttcg
    14581 gctcttcgta gatttcacgc ggcgtgccgt cttgctcaat gcgaccatcg cgcatcacca
    14641 caatcctgtc cgacatggtg agtgcttctt cctggtcgtg agtcacgaag acgaatgtaa
    14701 tgccaagctt acgctgtaac gctttcagct cgttctgcat ttgcttgcgc agtttgtaat
    14761 ccagcgctga gagcgactca tccagcaaca acagacgagg cttgttaacc accgcgcgag
    14821 caatggcgac gcgttgctgt tgaccaccag agagctgatg cggtttacgt tgagcgaagg
    14881 tttccagctg caccatccgc agggcttcca tcacgcgggg cgtaatttca gcagcggggg
    14941 ttttttgcat gcgcaaccca aaggccacat tttcgaacac ggtcatgtgg gggaaaagtg
    15001 cgtagctttg gaaaacagtg ttcacatagc ggttttccgc cggaacgtgg gtgatgtcct
    15061 cgttatccaa catgatgcgt ccggaatcaa cagtttccag acctgcaatc aggcgaagaa
    15121 cggttgtttt accgcagcca gaagggccaa gcagcgtgag gaactcgcca ttgttgatag
    15181 tcagatccag ctggggaatg acctctttac catcaaagca tttgcgaatt cccgccaatt
    15241 gcaccagcgg tgaaagcgaa ctcggttgtt tattcaattt tttactctgt cccatgtaaa
    15301 cgcaacggat ggcttaccga tgcggggttt gtggttaacc accttggtga ctcttaatga
    15361 gggcggtaat tctacggcaa accgcttgaa tcgccaatct ttgttgtgaa ttactggctt
    15421 agctttatat tcattaaggt aatgctgata aatattcccg cttgcagggg taaaagtgac
    15481 ctgacgcaat atttgtcttt tcttgcttct taataatgtt gtcacaaaaa gtgagggtga
    15541 ctacatggat aaactacttg agcgattttt gaactacgtg tctctggata cccaatcaaa
    15601 agcaggggtg agacaggttc ccagcacgga aggccaatgg aagttattgc atctgctgaa
    15661 agagcagctc gaagagatgg gccttatcaa tgtgacctta agtgagaagg gcactttgat
    15721 ggcgacgtta ccggctaacg tccctggcga tatcccggcg attggcttta tttctcatgt
    15781 ggatacctca ccggattgca gcggcaaaaa tgtgaatccg caaattgttg aaaactatcg
    15841 cggtggcgat attgcgctgg gtatcggcga tgaagtttta tcaccggtta tgttcccggt
    15901 gctgcatcag ctactgggtc agacgctgat taccaccgat ggtaaaacct tgttaggtgc
    15961 cgatgacaaa gcaggtattg cagaaatcat gaccgcgctg gcggtattgc aacagaaaaa
    16021 cattccgcat ggtgatattc gcgtcgcctt taccccggat gaagaagtgg gcaaaggggc
    16081 gaaacatttt gatgttgatg ccttcgatgc ccgctgggct tacactgttg atggtggtgg
    16141 cgtaggcgaa ctggagtttg aaaacttcaa cgccgcgtcg gtcaatatca aaattgtcgg
    16201 taacaatgtt cacccgggca cggcgaaagg agtgatggta aatgcgctgt cgctggcggc
    16261 acgtattcat gcggaagttc cggcggatga aagcccggaa atgacagaag gctatgaagg
    16321 tttctatcac ctggcgagca tgaaaggcac cgttgaacgg gccgatatgc actacatcat
    16381 ccgtgatttc gaccgtaaac agtttgaagc gcgtaaacgt aaaatgatgg agatcgccaa
    16441 aaaagtgggc aaagggttac atcctgattg ctacattgaa ttggtgattg aagacagtta
    16501 ctacaatatg cgcgagaaag tggttgagca tccgcatatt ctcgatatcg cccagcaggc
    16561 gatgcgtgac tgcgatattg aaccggaact gaaaccgatc cgcggcggta ccgacggcgc
    16621 gcagttgtcg tttatgggat taccgtgccc gaacctgttc actggcggtt acaactatca
    16681 tggtaagcat gagtttgtga ctctggaagg tatggaaaaa gcggtgcagg tgatcgtccg
    16741 tattgccgag ttaacggcgc aacggaagta agcgaaaagg gatgcggcat gtgatgccgc
    16801 atccggctta aatccaaact tacccttcga agaaccaata cccgctattg accagcgccg
    16861 cgagcatcgc gaggaatgac ggatcttcca gcgcatcgcc aaaattctcc gcggtcagcg
    16921 caatgttgct ggcgagtgca tccagtgccg gacggtgcgg ggaatcgatc ttctcaccat
    16981 tggcatacac gtcgtcgcca atgcgcaata cgcgcagacc acccaggcgc accagcacat
    17041 caccttgttt cagcgcatcg tagatttcat ccggctgata aggcggctct ggcggcgcga
    17101 tatccagttc atgacgtgac tgggatataa actccccaaa ccattgctta aagtgttccg
    17161 gctggttgat caattcgagc atcatctcac gcagtttatc catctcttgc ggcagaacat
    17221 ccgcaggatg agcgcgaggt ggaacatcag gatcgctgta gtagttgccg cccagttcac
    17281 gttgcagcac ataatcggca aatccactaa tcagttcccg cgtatttggc gcgcgaaaac
    17341 ccacggaata gttcatcgca ttttccagcg cgtagccttc atgcgggaat cctggcggaa
    17401 tataaagaat atcgccaggc tccagctctt catcgatgat ggcttcgaac ggatcgacct
    17461 gtaacagatc cgggtgcgga cagtgctgtt tcatttgcag cttttcgccc actcgccagc
    17521 gacgacgacc ggtaccctga atgataaaca cgtcgtactg atcgagatgc gggccgacgc
    17581 cgccgccagg tacagaaaaa gagatcatca gatcatcaat acgccagtcc ggtaattcac
    17641 ggaacggtcg catcagcgcg gcggtcggct catgccagtg gttcactgct tgcactaaca
    17701 atgaccagtt agtttcaccg agatgatcgt agctttcgaa cggaccgtgg ctgacctgcc
    17761 attttccatc ctggtgactg accagtcgac tgtcgacttc gctttccatc gccagacccg
    17821 ccaactcgtc tggagagatc gggtcaataa aattattaaa gccgcgtttt aacaccaccg
    17881 ggcgtttctg ccagtgacgt tcaagaaaat cgggccagtt aagagtgagt tggtattcca
    17941 tgttaagcat ccacaggctg gtatctgcaa ccgattataa cggatgctta acgtaatgcg
    18001 tgaagtatgg acatatttat tcatctttcg gcgtagaatg ctggcgacca aaaatcacct
    18061 ccatccgcgc accgccaagc atgctctctc cggcgacgat tttaccctca tattgctcgg
    18121 tgatttcgcg ggctaccgcc agccccacac cttgcccagg gcgtaaagta tcaacccgtt
    18181 gaccacggtc gaaaatgacc tctcgcttgc ttaatggaat accgggtcca tcatcctcga
    18241 ccacaatata gagatgctcg tcggtttgcc ttgcagaaat ttcgacaaac tcgaggcaat
    18301 atttacaggc attatccagc acattgccca tcacctcgac aaaatcgttc tgctcaccga
    18361 caaagctgat ctctggcgaa atatcgagag agatattgac ccctttgcgt tgatacactt
    18421 tgttcagcgc cgaggtgaga ttgtccagca gtggggcgac cggatgcagc tcgcggctga
    18481 gcaatgtccc gccgcgcata ctggcacgat gcaggtagta gccaatttgc tgtgaaatgc
    18541 ggctgatttg ctccagcatt accggctcag catcactgac gctcattttc tcactacgca
    18601 gagaacgcag cgtactttgc agcaccgcca gtggcgtttt cagactatgg gtcaggtcgg
    18661 tgagcgttgt acgatatttg tcgtaacgtt cgcgttcact ttttaacaat cggttcaggt
    18721 ttcgtaccag actggtcagt tctcgcgttg tggctggatt gagcaattcg cggttatgtt
    18781 cttccagttc gcggacttct tttgccaggg cttcgatggg gcgtaaactc caccaggcgg
    18841 cgacccacag cagcgggatc actaacagca gattggctga gagcacatag ataaaccagc
    18901 tccagaccat ataggaactt tttagctcca ccggaatggt atccaccacc acaatggtta
    18961 actttggcat ccgcgatgtt gccgggtaga cgtttaccgc caccgagtgg gtcatctccg
    19021 cgtcgtcatc atcttcccgc acttcctgca actgttgctg tatcgaatga tctccactca
    19081 gcaagaggct ggtatcgtta acatccgctt caatttcatg aaaaccattc gatttcagcc
    19141 agtcaggctg gatcatcttc atcagccagg gcacgtcacg ttgcgcccat aaaagctgcc
    19201 cgttctcatc ataaattagc gtcatggtgg ggctttgctt gtcgatattt tcgggtaact
    19261 cgacatgcaa cttattgttt tcccacttcg caagggtata gaacagattg ctctcgccac
    19321 gtaacagccg aaacgtagtt ttatcgaaac tgacgctata accgatcagc gcgaccattc
    19381 cgtaggcaag cgaaagcacc agtactaccg ctgccgttgc caacagaaaa cgtacccgca
    19441 gcgagagcgg gaaaaaaaga cgcagtaatt ttttcatcag cgcaattcga acagatagcc
    19501 ctggccgcga acggtggtaa tcacttcttg gggatattgt gcctgaattt ttttgcgcag
    19561 acgtcccatc agtacatcaa tggtatggct ttcccgcagc tccgcatccg gatagagttg
    19621 gagcattaac gaatctttgc tcaccacttt gccattattg cgtatcaacg tttccataat
    19681 ggtgtattcg aacgcggtca gtttgatcac ttcgtcatta atagataatt cacggcgaga
    19741 gagatcaacc tgaaacggtg ggagcgaaat gacctgtgaa gccaggccgc tattacgccg
    19801 cattaatgcc tgcattcgcg ccatcacctc ttcaatatga aacggtttag tcacgtaatc
    19861 atcagcaccg gcacttaata cttcgacttt gtcctgccag ctttcacggg cggttaatac
    19921 cagaatcggc agtgaaacat cattgctacg ccagcggcga atcagtgaca gaccgtcctc
    19981 gtctggcaat ccgagatcga caatcgcaat atccggtaaa tgttcattga gataataatc
    20041 ggcttctttg gcatcttctg catcatcgac ctgatgacca gcatcctgaa tctgaacttt
    20101 aaggtggtga cgtaacaacg cattgtcttc aacaaccagt acgcgcattt ttatttctcc
    20161 ctgtcttaat tattaaaata gtgtaacgcg attatgtggt tatgggggta aacattaaat
    20221 aaaccagcgg ggaggggagg taaagtgaaa aaataaaaag cggataatct taataagcag
    20281 gccggacagc atcgccatcc ggcactgata cgagtttatt tcagctcatc aaccatggtg
    20341 atggcgcgac caatgtagtt tgccggcgtc atcgctttca ggcgggcttt ctcttcttct
    20401 ggcaacgcca gaccgtcgat aaactgcttc atgccttcgg cgtcaacgcg cttaccacga
    20461 gtcagctctt tcaatttctc gtacggtttt tcgatgccat agcgacgcat aactgtctgg
    20521 attggttctg ccagcacttc ccagttgtga tccagctcat ccagcagatg gtcacggttc
    20581 acttccagtt tgctcacgcc tttcagggtg gattgatacg caatcagcgc ataaccgata
    20641 cccacgccga ggttacgcaa cacggtggag tcggtcaggt cacgctgcca gcgggaaacc
    20701 ggcagtttgc ttgccagatg ctgtaatacc gcgttggaaa ggcccagatt cccttcggag
    20761 ttctcgaagt cgatcgggtt aactttatgc ggcatggtgg aagaaccaat ctcaccggcg
    20821 atggttttct gtttgaagtg gttaagggcg atataacccc agacgtcacg gtcaaagtcg
    20881 atcagaatgg tgttgaagcg cgcaacgcaa tcaaacagtt cggcaatgta gtcgtgcggt
    20941 tcaatctggg tagtgtacgg gttccattga atacccagcg aggtgacgaa ctcttcgctg
    21001 aactgatgcc agtcaacttc cgggtaagcg gcgatgtggg cgttatagtt accgaccgcg
    21061 ccgttgattt tgccgaggat ctccacctgg ttaagctggc ggtactggcg ctccatacgg
    21121 taggcgacgt ttgccatctc tttaccgatg gttgacggcg tggctggctg accgtgggta
    21181 cgagacagca gcgggatatc gcggtactga acggcgagat ctttaatgcc atcaattagt
    21241 tgacgccagt acggcaggat cacttcatca cgcgcggttt tcagcattaa tgcgtgggag
    21301 aggttattga tatcttccga agtacaggca aagtggatga attcagaaac cgcgtgcagt
    21361 tccgggatct ccgccacttt ttctttcagg aaatactcaa ccgctttaac gtcgtggtta
    21421 gtagtacgct cgatggtttt gatgcgtgcg gcatcttctt cgctgaaatt ggcgacaatt
    21481 gcatcaaggt aaccgattgc gtcggcagca aaagcaggaa cttccttgat cgctgcgtgc
    21541 gcggccagtt tttgcagcca acgtacttca acttgtacac ggaatttcag caaaccatat
    21601 tcgctgaaaa tcccgcgcag cgcgctgact ttatcgccgt agcgtccatc gacaggggaa
    21661 acggcggtca gtgaggataa ttccatagat cacaactccg gggttaaatg agcaagaatt
    21721 tgttttgcct gagtggtcag gcgattacga gaaaacatca gttgcagacg tccgccgccg
    21781 acctggtgcc agagcacggc ggcgcgaatg cctgccagca gggttgcgcg aactttcgcc
    21841 tgcacttgtg ggctttgcag tacagcaggg gagccggtga cctgaatgcg cgggccaagc
    21901 gggctaatca catcaacata gatagcagcc atcgcgctca tcagcgtttc ggactgtaaa
    21961 tcgaagtgtt cgagctggcg ttgcaggccg ttgatccggt tgcccagagt gtcgagcgca
    22021 cctttcgctg aggagagttt gcgctcaagc accatcaggc tgagtgtgta gcgggttaat
    22081 tcggcgttta agccctggcg actgctggca ttgagcacgc cgagcagggt ttccagcccg
    22141 acgcgcaggt tggcttcgct accgccaaaa accgccagcg tcgagctggg gttcatatca
    22201 ataatactgt tgagtgagac gtgtagcgca tcggcatcac aatgcccctg gtgagcgagt
    22261 tgttgcacca ggcgtgccga ctgacaaata ccggccaggg cgagggtgat gtcatagtaa
    22321 ttctttgcca cgttcactgc ttccttgttt taagtaaaga taataatcag accggcagcg
    22381 gcagacgctg ctcaataata ccgccaccga ggcacacttc accgttatag aagacggcag
    22441 actggcccgg cgtcacggcg gcaacgggct catcgaaaat cacttcaatg cgatcatcgt
    22501 ccagcgcctt gacggtgcag gggatatcgg tctggcgata gcgggttttt accgtgcaac
    22561 gcatagtacc ggtgaatggt tcacgatcga cccagtgcag ctgctgggca atcagaccga
    22621 cagacatcag acgcgggtgt tcatggccct gcgcaacaat cagaatgttg ttttcgacgt
    22681 ctttgtccac cacataccac ggttcttcgg tgccttcttt ggtgccaccg atacccagac
    22741 ctttacgctg accgagggtg tgatacatca gcccctggtg ctcaccaatt tcatcgccgt
    22801 caacagtaat gattttgccc ggttgtgccg ggagataacg gcccaggaac tcgcggaatt
    22861 tacgttcgcc aatgaagcaa atgccggtag agtctttttt cttcgcggtg accagaccaa
    22921 gatcttcagc aatcttacgc acctgcggtt tttccagttc gccgaccggg aacaggcttt
    22981 gcgcaatctg ctcatggctg agcgtataaa ggaagtagct ctggtcttta ttgctgtcca
    23041 gaccacgcag caggcggctc ttgccatcga catcgacccg acgaacgtaa tgaccggtag
    23101 cgatataatc ggcacctaaa tcttcggcgg caaattcgag gaaggcttta aatttgatct
    23161 ctttgttgca gagaatatcc ggattcggcg tgcgaccggc tttatattcg gcaaggaaca
    23221 gttcgaagac gttgtcccag tactcggcag caaagttaac ggtgtgcagt tcaatgccga
    23281 gcttgtcgca gacagcctgg gcatcagcca gatccgctgc cgctgtgcaa tattcctcac
    23341 cgtcgtcttc ttcccagttc ttcataaaca ggccttcgac ctgatatccc tgttgttgca
    23401 acagccaggc agaaacggag gaatcaacac cgccggacat gccgacgatt actttttttg
    23461 cggtttcaga cattggatca ctcacgacat tgaacttcaa ggcggcgtat tctatcacgc
    23521 agccttttag atgacaccct ttgtaaaagg ccagttaaaa tcgccaatca tctccagcgg
    23581 atagcgttgc ccgctttgat aacaacgtat actttccgcc accagtggcg aacgaagatt
    23641 tgacgcctgt aaaatttctt cggcgctaac ccaacggcag cagtcgatat cgctgtcatg
    23701 aggctgggtc gggcatattt gctcaagctc aatggcaaaa aggaaacgca aaaacggcgt
    23761 tttatctggc gcaatccact gatgcatacg aataaagtgt tgcggctgcg cgctgatgcc
    23821 ggtttcttcc cacagctcac gggcggcggc ttccactaag gtttcatcgg cctccagatg
    23881 cccggcaggt tggttccata aggctttacc attaatcgtc tcttcaacga ctaaaaattt
    23941 gccttctgcg tgcaccacgc aagcaacggt aacgtgcggt ttaaacattt tgcatcctta
    24001 atctgtcact tctcgccatt cgccattagc aagattatcc aaagagtaat cacccatcgc
    24061 atagcgaatc agtcgcagcg tggggaaacc aacatgggcg gtcatgcggc gcacctggcg
    24121 attacgtcct tcatataagg tgatcttcag ccagctggtg ggaatacttt tgcgttcacg
    24181 aattggtgga ttccgcggcc ataaccacgc aggttcgtca accagctccg cgccggcggg
    24241 cagggtaggg ccatcattta aggttacgcc attgcgcaag gcttcaagtg cgtcttgtgt
    24301 gggaatgcct tccacctgca cataatagat ttttccggtg cgtttgcctg gctgggttaa
    24361 acgcgcctgt agtgcaccat tattggtcag caccaataac ccttcgctat cgcggtcaag
    24421 acgacctgct gcataaacgc cctgaaccgg gatgaattct tttaatgttt tgcgtccggc
    24481 ttcatcggtg aactgcggaa gaacatcgta gggtttattg aacaggatca cacgcgtggg
    24541 ctggttttca ggtttacgcc tggtagaacg ttgcgagctg aatcgcttaa cctggtgatt
    24601 tctaaaagaa gttttttgca tggtattttc agagattatg aattgccgca ttatagccta
    24661 ataacgcgca tctttcatga cggcaaacaa tagggtagta ttgacaagcc aattacaaat
    24721 cattaacaaa aaattgctct aaagcatccg tatcgcagga cgcaaacgca tatgcaacgt
    24781 ggtggcagac gagcaaacca gtagcgctcg aaggagaggt gaatggaaag taaagtagtt
    24841 gttccggcac aaggcaagaa gatcaccctg caaaacggca aactcaacgt tcctgaaaat
    24901 ccgattatcc cttacattga aggtgatgga atcggtgtag acgtaacccc agccatgctg
    24961 aaagtggtcg acgctgcagt cgagaaagcc tataaaggtg agcgtaaaat ctcctggatg
    25021 gaaatttaca ccggtgaaaa atccacacag gtttatggtc aggacgtctg gttgcctgct
    25081 gaaacccttg atctgattcg tgaatatcgc gttgctatta aaggcccgct gaccactccg
    25141 gttggtggcg gtattcgttc tctcaacgtt gccctgcgcc aggaactgga tctctacatc
    25201 tgcctgcgtc cggtacgtta ctatcagggc actccaagcc cggttaaaca ccctgaactg
    25261 accgatatgg ttatcttccg tgaaaactcg gaagacattt atgcgggtat cgaatggaaa
    25321 gctgactctg ccgacgccga gaaagtgatt aaattcctgc gtgaagagat gggcgtgaag
    25381 aaaattcgct tcccggaaca ttgcggtatc ggtattaagc cgtgttctga agaaggcacc
    25441 aaacgtctgg ttcgtgcagc gatcgaatac gcaattgcta acgatcgtga ctctgtgacc
    25501 ctggtgcaca aaggcaacat catgaagttc accgaaggcg cgtttaaaga ctggggctac
    25561 cagctggcgc gtgaagaatt tggcggtgaa ctgatcgacg gcggcccgtg gctgaaagtt
    25621 aaaaacccga ataccggcaa agagatcgtc attaaagacg tgattgctga tgcattcctg
    25681 caacagatcc tgctgcgtcc ggctgaatat gatgttatcg cctgtatgaa cctgaacggt
    25741 gactacattt ctgacgccct ggcagcgcag gttggcggta tcggtatcgc ccctggtgca
    25801 aacatcggtg acgaatgcgc cctgtttgaa gccacccacg gtactgcgcc gaaatatgcc
    25861 ggtcaggaca aagtaaaccc aggctctatt atactctctg ctgagatgat gttgcgccac
    25921 atgggttgga ccgaagccgc agacctgatt gttaaaggta tggaaggcgc aatcaatgcc
    25981 aagaccgtaa cctatgactt cgaacgtctg atggaaggcg ctaaactgct gaaatgttca
    26041 gagtttggtg atgcgatcat caagaacatg taatctctac atgtgttaaa tattgaaacg
    26101 ggcgtataac acgcccgttg ttttatttat gtggatatta ttaatagcat atcgagcata
    26161 ttta
//
LOCUS       DAZKFU020000042        26054 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZKFU020000042 DAZKFU020000000
VERSION     DAZKFU020000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.042"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..578)
                     /locus_tag="ACHOQQ_RS06910"
                     /old_locus_tag="pgaptmp_001382"
     CDS             complement(<1..578)
                     /locus_tag="ACHOQQ_RS06910"
                     /old_locus_tag="pgaptmp_001382"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGU4469655.1"
                     /translation="MSTLKPAIFSFFSGSGFLDLGFENSGYEVVFVNEFHPPFMDAYK
                     HSRAVMNKPIPRFGYAEESIEEVDHNKLKDNIKALKKEKRLIGFIGGPPCPDFSVAGK
                     NKGRDGENGKLSQSYINIIVEMKPDFFLFENVKGLYRTLKHREFFEHLKKQLMDVGYI
                     LHERLINSIEYGVPQDRERIILLGFQNKSLTK"
     gene            complement(1625..1786)
                     /locus_tag="ACHOQQ_RS06915"
                     /old_locus_tag="pgaptmp_001383"
     CDS             complement(1625..1786)
                     /locus_tag="ACHOQQ_RS06915"
                     /old_locus_tag="pgaptmp_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469656.1"
                     /translation="MTDRSPTEVIHGIIEEQEERDLILMIEEQRERQRRQEASQPDSV
                     SAENQADVP"
     gene            complement(2320..2418)
                     /locus_tag="ACHOQQ_RS06920"
                     /old_locus_tag="pgaptmp_001384"
                     /pseudo
     CDS             complement(2320..2418)
                     /locus_tag="ACHOQQ_RS06920"
                     /old_locus_tag="pgaptmp_001384"
                     /inference="COORDINATES: protein motif:HMM:NF017845.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
     gene            complement(2489..2791)
                     /locus_tag="ACHOQQ_RS06925"
                     /old_locus_tag="pgaptmp_001385"
     CDS             complement(2489..2791)
                     /locus_tag="ACHOQQ_RS06925"
                     /old_locus_tag="pgaptmp_001385"
                     /inference="COORDINATES: protein motif:HMM:NF017845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4469657.1"
                     /translation="MIRDDKAEALENSGLWRRAAERWGELFRQAKTDEERHYITQRRA
                     QCTAKATRPKTTEWGHVCAVRAAATRTLKDMGIDQKKEDPLRGTKYRGETGRRDGS"
     gene            2876..3262
                     /locus_tag="ACHOQQ_RS06930"
                     /old_locus_tag="pgaptmp_001386"
     CDS             2876..3262
                     /locus_tag="ACHOQQ_RS06930"
                     /old_locus_tag="pgaptmp_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469658.1"
                     /translation="MRLTQYKKEILSYFTPDNLSWVRGEIGNPPFDVSGVAYLLYGID
                     SHNNRYQLESTRRTLEAMVRDGLLERINVYERRDSVGHGVRRKVARYGLPGRSVVARD
                     GEGRNGAIDGECIRINATDVVLVEGQ"
     gene            complement(3713..4006)
                     /locus_tag="ACHOQQ_RS06935"
                     /old_locus_tag="pgaptmp_001387"
     CDS             complement(3713..4006)
                     /locus_tag="ACHOQQ_RS06935"
                     /old_locus_tag="pgaptmp_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4469659.1"
                     /translation="MIADSVAQKLEERGLWRRAATRWSDVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFADVKKAADRTLKEMGINPQDEWKNYSFSDAGDDLALS"
     gene            complement(4026..4310)
                     /locus_tag="ACHOQQ_RS06940"
                     /old_locus_tag="pgaptmp_001388"
     CDS             complement(4026..4310)
                     /locus_tag="ACHOQQ_RS06940"
                     /old_locus_tag="pgaptmp_001388"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGU4469660.1"
                     /translation="MFGAENIKPLKAGVIHDMLQDAKARELAIGHGVLRGALTSYIHT
                     TRYLKAIVAGGARYDLNGQPCGEVTEEDKSVASELLKRRLEQMKQKNSQQ"
     gene            complement(4459..4908)
                     /locus_tag="ACHOQQ_RS06945"
                     /old_locus_tag="pgaptmp_001389"
     CDS             complement(4459..4908)
                     /locus_tag="ACHOQQ_RS06945"
                     /old_locus_tag="pgaptmp_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4469661.1"
                     /translation="MTAQIAAYGRLVDDPQEKQTSKGTPMTLTRMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGETRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGTRGNQPPAGGDDPFSDDIPF"
     gene            complement(4905..5354)
                     /locus_tag="ACHOQQ_RS06950"
                     /old_locus_tag="pgaptmp_001390"
     CDS             complement(4905..5354)
                     /locus_tag="ACHOQQ_RS06950"
                     /old_locus_tag="pgaptmp_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469662.1"
                     /translation="MTEKHAKKSHSHAIDLTEPESVVRFVSVRGRATLYIPDEHLHHC
                     DKHKLPILIVWKRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERTGKIYEKYAANS
                     KRAARAITYHFMTLYDLKADDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            complement(5633..7456)
                     /locus_tag="ACHOQQ_RS06955"
                     /old_locus_tag="pgaptmp_001391"
     CDS             complement(5633..7456)
                     /locus_tag="ACHOQQ_RS06955"
                     /old_locus_tag="pgaptmp_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HGU4469663.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWLEQDGKLA
                     GDNIPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKERGKGDDATSLALNQMGAS
                     QRGEVLLAHYGGELVINADSDTVHYYNSVVWVPVQDKELQRTMAQIFIDEDVCYSQNA
                     IKSAVDTMKLSLSVMGNTARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFTPPA
                     EGETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGK
                     SVMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAIT
                     GGDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEIVPENER
                     DPMLPEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGY
                     LMSSVMCDGLLVGNAEIIPFSPRRYLYHAYLSYMRAHGFSKPVTLTRFGADMPGAMAE
                     YGREYMKKRTKEGVRSNVILTDESEDWLPSCAPVKNEGDKN"
     gene            complement(7462..7575)
                     /locus_tag="ACHOQQ_RS06960"
                     /old_locus_tag="pgaptmp_001392"
                     /pseudo
     CDS             complement(7462..7575)
                     /locus_tag="ACHOQQ_RS06960"
                     /old_locus_tag="pgaptmp_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(7642..7836)
                     /locus_tag="ACHOQQ_RS06965"
                     /old_locus_tag="pgaptmp_001393"
     CDS             complement(7642..7836)
                     /locus_tag="ACHOQQ_RS06965"
                     /old_locus_tag="pgaptmp_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469664.1"
                     /translation="MSDLFDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDMQ"
     gene            complement(7840..8025)
                     /locus_tag="ACHOQQ_RS06970"
                     /old_locus_tag="pgaptmp_001394"
     CDS             complement(7840..8025)
                     /locus_tag="ACHOQQ_RS06970"
                     /old_locus_tag="pgaptmp_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469665.1"
                     /translation="MLKTFRVFSRAVNARGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDKE"
     gene            complement(8018..8206)
                     /locus_tag="ACHOQQ_RS06975"
                     /old_locus_tag="pgaptmp_001395"
     CDS             complement(8018..8206)
                     /locus_tag="ACHOQQ_RS06975"
                     /old_locus_tag="pgaptmp_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4469666.1"
                     /translation="MATIPALVHSQTAFIWRFIIFGASESQIINVTAWTEREARNRCP
                     SGCVAVFAARIRQEVTHA"
     gene            complement(8237..10072)
                     /locus_tag="ACHOQQ_RS06980"
                     /old_locus_tag="pgaptmp_001396"
                     /pseudo
     CDS             complement(8237..10072)
                     /locus_tag="ACHOQQ_RS06980"
                     /old_locus_tag="pgaptmp_001396"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            complement(10059..10859)
                     /locus_tag="ACHOQQ_RS06985"
                     /old_locus_tag="pgaptmp_001397"
     CDS             complement(10059..10859)
                     /locus_tag="ACHOQQ_RS06985"
                     /old_locus_tag="pgaptmp_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HGU4469667.1"
                     /translation="MKRPNMPVQGQGFVQPEELSQQYFAAIIPVSPGIIGGYETGIVS
                     ARALHKALGVGRDFTNWIKGRIEEYEFKKGMNFEVVENLSSPNPASAKSRQQLAHDYL
                     LTVNMGKELAMVERTEQGRAIRQYFIKCEEELHKAAPERAAALRRELKARVTVASFFK
                     PMCAALELYRVELGKETHQHHYTTESNMLARIVLGGMTAKQWAQANGIKGKPRDSMSV
                     MQLEHLSYLEQSNITLIELGMTYQQRKAELIRLSQRWLARRMGEHEHA"
     gene            complement(10927..11142)
                     /locus_tag="ACHOQQ_RS06990"
                     /old_locus_tag="pgaptmp_001398"
     CDS             complement(10927..11142)
                     /locus_tag="ACHOQQ_RS06990"
                     /old_locus_tag="pgaptmp_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4469668.1"
                     /translation="MTANHQEAIPTTGYIRRCNLAKLLDTSVSTIDRWVRIGKLPKPT
                     KLGEKNTVFNAVEINHWLNERRNNTKQ"
     gene            complement(11529..12179)
                     /locus_tag="ACHOQQ_RS06995"
                     /old_locus_tag="pgaptmp_001399"
     CDS             complement(11529..12179)
                     /locus_tag="ACHOQQ_RS06995"
                     /old_locus_tag="pgaptmp_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469669.1"
                     /translation="MINILKKSPIERLCASVSITPHEMALALADLNPSMRIGDVPQDK
                     FEQVESARTHIARAIRLHSGVKTSKDEPYRAGDIFLASFPFMEAGTPEAIIKAVTDTI
                     DDLRGTKNWEEKALNLGGRDLVRYIKETSRSGRGQHRKLDEEQGNMKMMGLLVLLLVK
                     KSGTTAYIQDGEPNRSAIYRDVEALMKEKGISPKGIAKSTFMQKISAALLAVSQAD"
     gene            complement(12176..13438)
                     /locus_tag="ACHOQQ_RS07000"
                     /old_locus_tag="pgaptmp_001400"
     CDS             complement(12176..13438)
                     /locus_tag="ACHOQQ_RS07000"
                     /old_locus_tag="pgaptmp_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4469670.1"
                     /translation="MPKLTDIQIRAWIKADERFDGKADGNGLYLCYPKNYTMPFWRFR
                     YKFAGKQRAMVIGSYAELSLSRARETAKELSARVALGYDVAAEKQERKAEALAKMEAE
                     KNAIRVSELAAEYFERQILPRWKHPDILRRRIDKDINPRIGHMKVEDVKPRHVDEMLK
                     AIVDRGAPTIATDVLRWTRRIFDYGVKRHVLEINPCSAFEVSDAGGKESSRDRWLTRE
                     ELIRFFQAMRTAKGFSRENELTFKLLLALCVRKMELCAARWEEFDLDNAVWHLPEERS
                     KNGDPIDIPLVHPVIEWLRELRVLSCNSAWVLPARKMQHRMIPHIQESTLPVALAKVR
                     AEMPEVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRYQYFNERRD
                     ALAKWTNLLVSLERGEHYNVTPIRRAKS"
     gene            complement(13630..14493)
                     /gene="yicC"
                     /locus_tag="ACHOQQ_RS07005"
                     /old_locus_tag="pgaptmp_001401"
     CDS             complement(13630..14493)
                     /gene="yicC"
                     /locus_tag="ACHOQQ_RS07005"
                     /old_locus_tag="pgaptmp_001401"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGU4469671.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKALIEQRL
                     EGVTAEVVKVRSHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNVE"
     gene            14620..15336
                     /gene="rph"
                     /locus_tag="ACHOQQ_RS07010"
                     /old_locus_tag="pgaptmp_001402"
     CDS             14620..15336
                     /gene="rph"
                     /locus_tag="ACHOQQ_RS07010"
                     /old_locus_tag="pgaptmp_001402"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGU4469672.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            15402..16043
                     /gene="pyrE"
                     /locus_tag="ACHOQQ_RS07015"
                     /old_locus_tag="pgaptmp_001403"
     CDS             15402..16043
                     /gene="pyrE"
                     /locus_tag="ACHOQQ_RS07015"
                     /old_locus_tag="pgaptmp_001403"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGU4469673.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(16080..16676)
                     /gene="slmA"
                     /locus_tag="ACHOQQ_RS07020"
                     /old_locus_tag="pgaptmp_001404"
     CDS             complement(16080..16676)
                     /gene="slmA"
                     /locus_tag="ACHOQQ_RS07020"
                     /old_locus_tag="pgaptmp_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGU4469674.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(16783..17241)
                     /gene="dut"
                     /locus_tag="ACHOQQ_RS07025"
                     /old_locus_tag="pgaptmp_001405"
     CDS             complement(16783..17241)
                     /gene="dut"
                     /locus_tag="ACHOQQ_RS07025"
                     /old_locus_tag="pgaptmp_001405"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGU4469675.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(17219..18439)
                     /gene="coaBC"
                     /locus_tag="ACHOQQ_RS07030"
                     /old_locus_tag="pgaptmp_001406"
     CDS             complement(17219..18439)
                     /gene="coaBC"
                     /locus_tag="ACHOQQ_RS07030"
                     /old_locus_tag="pgaptmp_001406"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGU4469676.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            18611..19279
                     /gene="radC"
                     /locus_tag="ACHOQQ_RS07035"
                     /old_locus_tag="pgaptmp_001407"
     CDS             18611..19279
                     /gene="radC"
                     /locus_tag="ACHOQQ_RS07035"
                     /old_locus_tag="pgaptmp_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU4469677.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            19496..19732
                     /gene="rpmB"
                     /locus_tag="ACHOQQ_RS07040"
                     /old_locus_tag="pgaptmp_001408"
     CDS             19496..19732
                     /gene="rpmB"
                     /locus_tag="ACHOQQ_RS07040"
                     /old_locus_tag="pgaptmp_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGU4469678.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            19753..19920
                     /gene="rpmG"
                     /locus_tag="ACHOQQ_RS07045"
                     /old_locus_tag="pgaptmp_001409"
     CDS             19753..19920
                     /gene="rpmG"
                     /locus_tag="ACHOQQ_RS07045"
                     /old_locus_tag="pgaptmp_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGU4469679.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            20018..20827
                     /gene="mutM"
                     /locus_tag="ACHOQQ_RS07050"
                     /old_locus_tag="pgaptmp_001410"
     CDS             20018..20827
                     /gene="mutM"
                     /locus_tag="ACHOQQ_RS07050"
                     /old_locus_tag="pgaptmp_001410"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGU4469680.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(20866..21345)
                     /gene="coaD"
                     /locus_tag="ACHOQQ_RS07055"
                     /old_locus_tag="pgaptmp_001411"
     CDS             complement(20866..21345)
                     /gene="coaD"
                     /locus_tag="ACHOQQ_RS07055"
                     /old_locus_tag="pgaptmp_001411"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGU4469681.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(21353..22630)
                     /gene="waaA"
                     /locus_tag="ACHOQQ_RS07060"
                     /old_locus_tag="pgaptmp_001412"
     CDS             complement(21353..22630)
                     /gene="waaA"
                     /locus_tag="ACHOQQ_RS07060"
                     /old_locus_tag="pgaptmp_001412"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGU4469682.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSSSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            23043..24101
                     /gene="rfaQ"
                     /locus_tag="ACHOQQ_RS07065"
                     /old_locus_tag="pgaptmp_001413"
     CDS             23043..24101
                     /gene="rfaQ"
                     /locus_tag="ACHOQQ_RS07065"
                     /old_locus_tag="pgaptmp_001413"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGU4469683.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTSLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            24098..25222
                     /gene="waaG"
                     /locus_tag="ACHOQQ_RS07070"
                     /old_locus_tag="pgaptmp_001414"
     CDS             24098..25222
                     /gene="waaG"
                     /locus_tag="ACHOQQ_RS07070"
                     /old_locus_tag="pgaptmp_001414"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4469684.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVEANCGEAIVEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            25215..26012
                     /gene="rfaP"
                     /locus_tag="ACHOQQ_RS07075"
                     /old_locus_tag="pgaptmp_001415"
     CDS             25215..26012
                     /gene="rfaP"
                     /locus_tag="ACHOQQ_RS07075"
                     /old_locus_tag="pgaptmp_001415"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGU4469685.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPSDVRVKRMLIKRVATMVRNMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         6231 a         6505 c         6433 g         6885 t
ORIGIN      
        1 atcttggtta gacttttatt ttggaaaccc aacagaataa tgcgttcacg gtcttgcggg
       61 actccgtatt caatcgaatt tattaacctt tcatgaagga tgtaaccaac gtccattaac
      121 tgttttttga gatgctcaaa aaactcacgg tgtttgagcg tgcgataaag acctttgacg
      181 ttctcgaaca agaagaagtc aggcttcatc tcaacaatta tattgatgta actttgagac
      241 agcttaccgt tctctccatc gcgacctttg ttcttacctg caacggagaa atcagggcac
      301 ggtgggccac cgatgaagcc aattagtctt ttttctttct tgagagcttt aatattatct
      361 ttcaacttat tgtgatcaac ttcctcgatg ctctcttctg catatccaaa acgtgggata
      421 ggtttgttca tcaccgctcg tgagtgcttg taagcatcca taaatggtgg gtgaaattca
      481 ttcacaaaaa ccacctcgta gcctgagttt tcgaagccaa gatctagaaa acctgagccg
      541 gaaaagaaag agaagatggc gggtttaagt gtggacattt tttacctatc ggtgatcgtt
      601 gagtcatgca aaaacgcccc tgcatcttac cagatgaaag gggcattgca acgggattgc
      661 aggctcttgt aaaggtcgtc cgttgtcatt tttatggata tagtctttaa tcaacgtgtt
      721 cacctcctat gatgtgttta aggttgctat gtcttatgct gattgctgta aggtccatca
      781 gccaaataca tctctcgatt gtgagggatg taaaaatcat gtctggtcag gtcttgtttt
      841 caatgatgtg actgtcttta agacaggtgg ttcacatacc cagatatcgg ggatgtacaa
      901 atcaggggtt acggctggtg gatttgctca aatcaaatag agttcaagca atcgaataaa
      961 ttagccaaaa atcaattgtt taggtgcgag caacagagat tatcgacttt ttgcattgca
     1021 ataagttgaa taagcgggca aggccaaagc tcaaaaaaga gcgctggaac aatatcagca
     1081 agttaccgcc gtcagctacc cggctcagaa ttgagccgcc ttttaatcaa caagttaccg
     1141 ccgcaaccgc tccggcgtct tccactggcg gcctgattgc gctatccctt cacaagcaag
     1201 agcggcgtgc ccgctgctaa aatattacga tcgccgataa aaacaaaagc ctccacgcgg
     1261 gaggctatgt tgactcagcg ttgaccaagc ttgttatcgc tgcggtcacg ctggctacaa
     1321 tggacgcacc ttagagtagt ctcaaatgcc tgtcatcaga tattactgac gcaggagaca
     1381 atttaagcct accataaaat gcttggtttt aggcattgag tgcgctggca atttgtagtg
     1441 atagttccgt atgacatccg gctctactgt actgcaagca acaacagtgg ccaggttagc
     1501 cctgcccgct atatttccag ataaaatgat tactggcctt gatgatttga ccatttccgg
     1561 ctcttttttg caactcgttt aaaactggtc acagagtgac tttaagttac tcagttcgac
     1621 accatcacgg cacatcagcc tgattttcag cgctcacgct gtccggctgc gaggcctcct
     1681 gtctgcgctg gcgttctctc tgctcttcaa tcatgaggat taaatcgcgc tcctcctgct
     1741 cttcaataat cccgtgaatt acctctgttg gtgaacggtc ggtcataggt ccctccctgt
     1801 gagttgctgt tatgccagga atgttaacca gtactctcgt ttcctgccct gtaaaaacgt
     1861 tcatttttgt aggttacttt aagtaactta caaaaaatcg atcatcacgt atcggtttcg
     1921 ttttgcgctt cctgttgcgt ttcgtttttg agaaaaatgc gcagtttttt gcgcactttt
     1981 tgaggtgtct atccccctgc aagccgcgtc atatctagct ttgctctgtt tgtctgtgtg
     2041 atcgattttg tgatcggttt tatgatcgat ttgcgcactt ttttgcgcat tttttcaggg
     2101 atttttcacc ccatcgaaga ggcttctaag gggctgtgaa gtgggtagca ttttgctgtg
     2161 atggaaggac taccgctact gtctgataat ggtgacggag gccgctatgt tcggctctga
     2221 ttgcagcctg ccgtatattc accaatttac ctggctgtta tttccataac ccgtaatgtc
     2281 gccgatcccg tctgattccc atatcgtcaa cagtcttatt tacagcatcc tgaagttcgc
     2341 aaggcatatt tcccgccgtc tggctatatg ctctcgctgc ggatcggtgt gggcctcttt
     2401 cattacgtca agccaacgag cggcagctct tcaccatagt ccctgagatt ccagttgttc
     2461 ggctttttta tcagctattg tgtccatatt aacttccgtc tcttcgccct gtttcacccc
     2521 tgtacttcgt gccccgtaaa ggatcttctt ttttctggtc aatccccata tctttaagcg
     2581 ttctggtcgc tgctgccctg acggcgcata catgccccca ctctgttgtt tttggtctgg
     2641 tggcttttgc cgtgcattgc gcccttcgtt gggtaatata gtgcctctct tcatcagttt
     2701 ttgcctgtct gaacaattct ccccagcgtt cagcagcacg ccgccagagg ccgctattct
     2761 ccagcgcttc agccttgtca tcacgtatca tctgtacccc gccgattctg tcctttcaaa
     2821 atttgtctta tggcttattg tatgagggat tcatcctaca acaagaaagg tcactatgag
     2881 actgacgcaa tacaaaaaag agatcctcag ttacttcact cctgacaatc tgtcgtgggt
     2941 cagaggtgaa attggtaacc cgccgtttga tgtctccggt gtggcttacc tgctgtatgg
     3001 catagattca cacaacaacc gatatcagct cgaatcaaca aggcgcacgc ttgaagcaat
     3061 ggtgcgtgat ggtcttcttg agcggattaa tgtgtatgag cggcgtgaca gcgttggtca
     3121 tggcgttcgt cgaaaggtgg cgcgttatgg cctgccaggt cgttctgttg ttgcgcgtga
     3181 cggtgagggc cggaacggtg caattgacgg cgaatgtata cgcatcaatg ctacagatgt
     3241 ggtgctggtg gaggggcaat aaaaaaccct gaataactcc gctgggtgct tcaattgcac
     3301 ggggcgaaat cattcagggt cagctgtcac gggcctctgt acagagggct ccagttgccc
     3361 agagatgagc acccgctaca gcctgctgtg ataagccgct ttacagtgag cttcaattgc
     3421 tcaaagaggc gcgtttttat cacaacctga ccgcgataac ccgctgtaca gagggctcca
     3481 gttgcccaaa gaggcgcatt cttatcgcgg catgacgaaa tcaccgtgat gttttcgtcg
     3541 ttcactcatc ccgatgtgtg tgacaagtac acatcaaaat gagtgccgaa aagccgctgg
     3601 ctggtttcat tcggcgaggc tcccgttgcc tgcggagaac accatcaacg gcctttcggt
     3661 ggtgattata gcgaatcgcg ataattgagc aaatacggcg atgagatgcg tatcacgata
     3721 gcgctaaatc gtcgcctgca tcactgaagc tgtaattttt ccattcatcc tgcggattaa
     3781 tccccatctc tttcagcgtt ctgtctgcgg cctttttcac atcggcaaag gtgacgaact
     3841 gctcaggcat acgacgggat ttaatgatac atatcgcccg acgtctggca gcttcctcgc
     3901 gttccctgtc cgtttctgcg tgaagcagga cgtcagacca tcgggtcgcc gcacgtcgcc
     3961 ataaaccgcg ctcctcaagt ttctgtgcca cgctgtccgc tatcactggc tttacctccg
     4021 gctgattact gctgactgtt tttctgcttc atctgctcca gtctccgctt cagtagttcg
     4081 ctggctactg atttatcttc ttccgtgact tcaccgcaag gttgcccgtt caggtcatac
     4141 cgtgcgccgc cagcgacaat ggctttcagg tatcgcgtcg tgtggatgta tgaggttaat
     4201 gcgccacgta atacgccatg accaatcgcc agttcccgcg ccttcgcgtc ctgcaacata
     4261 tcgtgtatca cgccagcctt aaggggtttt atattctctg ccccaaacag ttcaggtcag
     4321 tactcagcca gtcggtttat cctcctgcgt ttttttcgct gccgctttgt catgtgctgc
     4381 cacggtatgc gccgtgatgg tttacgtgtg gtctggtcct gttgtgtggt ctgctggttc
     4441 atgttgtgct cctgtcattc agaacggaat atcgtcagag aacgggtcat cacctcccgc
     4501 tggtggctga ttcccccgtg ttccggtctg tcgttttgct ctttccagtg cttcggtcgc
     4561 cttcccctgt tgcccccgct tcccgccagg acgcgccgaa cgggcactga ttacgctgtc
     4621 agcgataacc tgccagccct gccgtgtttc tccgttctgt ccggtccact gactgatctg
     4681 catgttaccc gccacgctga taaggtcgcc tttttcatgt ctcgccagcg cgtcggcctg
     4741 cttaccaaat gccaggactg atagccataa cgtcgcctgt ccgtcctgtg cctgactgca
     4801 tggcaaagat accgccatac gcgtcagcgt catcggtgtg cccttgctgg tctgtttttc
     4861 ctgcgggtcg tccaccagcc gcccgtaagc tgcaatctgt gctgtcatgg tctgcgctcc
     4921 ttgttccggt actcctggat aatacccatt gtcatatcga ataaatcaca tgccgctttt
     4981 tctgcatcat ccgctttcag gtcatacagc gtcatgaaat ggtaagtgat cgcccgtgcg
     5041 gctcttttgc tgtttgctgc atatttctca tagatttttc ctgtccgctc ttcaatatcc
     5101 cgccggaact cttcacgttc aaacaggtca cggtgtatca acatcagcga atcattcagc
     5161 catgtcacat cggcgtatac cgttcgcttc cagacaatca ggatcggaag tttatgtttg
     5221 tcgcagtgat gcagatgttc gtcaggtata taaagcgtgg ctctgcctcg taccgatacg
     5281 aacctcacaa cagattccgg ttcggttaaa tcgatagcgt gtgaatgtga ttttttggcg
     5341 tgtttctctg tcataaaatt tttctcagag gaataaataa aaatttatgg caaaaaactg
     5401 ttcaactgtt caccttttgc tatttcgcct ttagattcat gaagttaagg ggtgaacact
     5461 ttttgtaaag gtgttcacca actgttcacc cgtgactgtt cgaaaatcat acaaaaaagg
     5521 tgaacaggtg aatactttgg tgaatagttt tacaaaactg ttcacccctt aacatattga
     5581 tactaaatac atttattgca gggtgaacag tggtgaacag tttttctata acttaatttt
     5641 tatcaccctc atttttcact ggtgcacatg acggcagcca gtcctcagac tcatccgtca
     5701 gtatcacgtt tgatcgcaca ccttctttag ttctcttttt catatactcc ctgccatatt
     5761 ccgccattgc gcctggcata tctgcaccga agcgcgtcag tgttacgggc ttactgaaac
     5821 cgtgcgccct catatacgag agataggcgt gataaagata acggcgtgga ctgaaaggaa
     5881 taatctcagc attaccgact aacaggccgt cacacattac cgacgacatg agatagccgc
     5941 agaagtccac cagcgaatcc ccctcacgct ttatcgccag tgcttcttct gatttctgct
     6001 gctcatataa aaggcgtctg gcttcgtcct gatcagcaaa ccgtgtaagc agatgacgaa
     6061 tcaccacagc aagctcacct tctatttttt ccggcagcat cggatcgcgt tcgttttccg
     6121 gtacaatctc cgaaaaattg aatatcacac gacgacgtga gatccccccg ctgcggtcac
     6181 tgaatgacat ggcgctgttg ttaaccgcca gcacaaccgc cggaatacgc gttgagtatg
     6241 gcgctttgtg tttcgggtca attgccacct tgtcaccgcc cgtaatggcc tttatccctg
     6301 caccatcacc agcgtagcgg gtcatatccg gcagaataat cagtgaaaag ccaaccacta
     6361 atgcgcgttc ccttgcatcc tccagtgcct tcatgcttgc cgataccgtg ttggccttac
     6421 ctgccagcat ggtacaaatc tccgccataa cacttttacc gcttccacca ggacctgtta
     6481 cctcaatgaa taactgccag tcgtaccggt tcgccagcac cataaataac gctgccagaa
     6541 cgcgatctgc cttgcggtca ttctcagcca ccgaacggcg taaccacttc cagaaattcg
     6601 gcgcatgtgt tgccagcgtt tcaccctctg ctggtggggt gaacggtaat tcactggcaa
     6661 ttaacagcca gtcattttta tcgtgttcac ggaaatcacc tgtacgggta tcaaataccc
     6721 cgttactgaa tccaatcagg ttacgggcgg tattccccat tacggataaa cttaacttca
     6781 tggtatcaac cgccgatttt atggcgtttt gtgaatagca gacatcttcg tcaatgaaga
     6841 tttgcgccat tgtgcgctgt agctctttat cctgtacggg aacccataca acactgttgt
     6901 aataatgcac tgtgtcagag tcggcgttta ttaccagttc gccgccataa tgtgccagaa
     6961 gaacttcgcc gcgctggctt gcccccatct ggtttagcgc cagtgatgtg gcatcatcac
     7021 cttttccccg ctcttttttc tttacaggca gctcaataac cagcccgtca gaaatgtttt
     7081 tgcgctcagt ctccagatag ttacgccagt tctgccgctc gttatggtgc attccttcgg
     7141 ggtagtaatc agcatcctgt acgcctgccg ccgccagctt ctgacctatg cctttgacca
     7201 tcacctgatc gaggtgtccg gccttgtata tccgcgctga tttgcgcccg tcgggtacaa
     7261 tttgcagatt gccaatatct ttaagttgct catcagccag cacgacaggc ggtatattat
     7321 ctccggcaag ttttccgtcc tgctccagcc actgtttcgc gtgcgcccac gcatcactgc
     7381 cagcgaaaat aatcacttcc gtatcggcgt gttttttccc tcgaggctgt ttttttaagt
     7441 tcggtgcgct tttcatttct tacctctgct gatcatcgtc atgtggctac cgtattcatt
     7501 gcggtaaata tccccgcaca taaattcggg gtgagttttg ctactggcag ttaagccaga
     7561 atatttacgt ttcatcgttt tatttcctga tatgtaacgc tttaattcgc tggtatttat
     7621 ttcatcctgt tacttcttcc cttattgcat atcacccgat gcagcaatca gccagctacc
     7681 tgccttacct gtaagctgct taatcagggc gagcaatgac tcaatctcac cctcagtcat
     7741 ttcacctgtg ttacccatca tcagagaaca aataagttct gcctgcattg cgctttttgc
     7801 tgcttcatca acagtcatat cgaaaagatc actcatggat tactccttat cctgaataca
     7861 tgtaacggcg tggataatta cgccggataa accggcttta gctgatttgt taattactgt
     7921 gctaattgcg gcctggacgt tttcggcctt cacgttctgc gtgatgccaa cggtatagcc
     7981 gcgtgcatta acggcacggg aaaagacgcg gaaggtttta agcatgagtc acctcctgac
     8041 ggatacgggc ggcgaataca gcaacgcaac ctgacgggca acggttacgc gcttcgcgtt
     8101 ccgtccaggc ggttacgttg atgatttggg attctgacgc gccaaagatg ataaagcgcc
     8161 agataaaggc agtttgagaa tgtacgaggg cagggatagt agccatgttg gcagcctcct
     8221 tacagtgatg aataacgtca ccgcaggagt tctcacgctc acacagggcg gtgacactgg
     8281 cgggggtgag aaaaccggac tgtaaggact ccggcctacc tttcggtagc cccgccagca
     8341 tcaccattgt ttgctggtgg cgctgtgcaa aatgcaactc gtgaaatgac tggatggtgc
     8401 acgagtacgc acaatacaca atgccattga tatggcgtaa agcgctggca gaaaaaaaga
     8461 cgcttctggc gtcatttatc gccttacagt taaacgggtt ctcacgcccg atccccgttt
     8521 tataagggat agcctcagta tatgccgctt ttttgcttaa gtggtcggat gtgttgcgag
     8581 agaagtttgt gcgaaaaata ttcacatatc cttccagaaa ggatggatac cagaaagaaa
     8641 gacaacgtac cagaaaataa ccagcgccgt gatatgaggc atcacgacat aatgcattac
     8701 cggaatccag tatgagtaag cacagatttt cgcccgttac cccgaatatc agcgcactac
     8761 aacgccagcg actgatacgt gacatcattg aaaccaggca agaaaaatta accccgccag
     8821 aaaaaaccag cgaaccgcca ccgcttgagg tgtttctcag tggcagaaaa aaccaacgca
     8881 acaaattaca acaacagaaa ccatcatcgc tgtagcgtgg tgtgttaggg cgcattgctt
     8941 tacagggcag cagcatgaac aaaaagtacc aatccattac agatattttt gccgaacaac
     9001 gaagacagct tttgcaccgc atacttgagc gccaactgga actgaaacgc gctaaagaga
     9061 tatcagaacc cgaagagtta acgcctcttc ccggagtgaa aaaacaccca caaaaacgat
     9121 ttgcccttga tgactttatt gagaaatacc cacggcggtt aaaagccact aaaaaccgac
     9181 cgccaggctg atagctcgct gtttcttctg cggctctatg aaacagacag aaaccatcag
     9241 cgccgtgatg tgaggaagta cggcgcattg ttttacaggt gcacaatatg gataacacac
     9301 aacacacttc acaatcagag aataaaaaag atgatctcac agcatatgag caacatttcg
     9361 ttgaaaaaaa acagtcaata acgagacaca tcgcagatat ggatatgcgc aagcgatttg
     9421 aaaaaacttt caataccaat tttgcgcttg aggatttttt aaagcagcat tccagccgat
     9481 caagcaataa gggatagctt atggatgacc agaaaaaaaa ctcctttgcc atgatggtta
     9541 cactgaccag cgcccgtata actgaacatg aacgaaagcg gaaagctgca cgcgaacagg
     9601 caagagcaac agaattaaca cgtaaagaac ttaacgctct ggcacgggaa cacagagaag
     9661 cacctgaagc ttttgcactt gataattttc tgaaaagtac atgccgtaag aaaatacgaa
     9721 catcgggacc aaggggccat tcgtcgtaaa ctgcatattg tccgccagcc tcagatatgg
     9781 cattgtggat aatgcgcttg atttccttta tctccactgg cgaccctttc gtggtcgcct
     9841 ttgttttgcc tgccattcct gcatcatgcg gatattcgcg ccgtacaggg ccaccagcgc
     9901 gataatttga taaaacctcc ctcaagtatt gcttaacgtg tttcatgcgc tcagcggcga
     9961 aatggctcac gatatggcgc atgtaatccg catggttgat gttcagtagt gcgaacttat
    10021 ccgccgtgtc gatgtaccgg acagcaggtt tgaggatgtc aggcatgttc atgctctccc
    10081 atgcgacggg ccagccagcg ctgtgaaagg cggattaatt cggcttttcg ctgctggtaa
    10141 gtcataccca actcaatcag cgtgatattg ctctgctcaa ggtaggaaag gtgttcaagc
    10201 tgcataacgc tcatgctgtc acgcggtttt cctttgatgc cgttcgcctg cgcccattgt
    10261 ttcgccgtca tgccgcccag cacgatacgc gccagcatat ttgattctgt ggtgtagtgg
    10321 tgttgatgag tctcttttcc cagctctacg cggtacaact ccagcgcagc gcacataggc
    10381 ttaaaaaaac tggcaacggt gacacgcgct ttcagttcac ggcgcaaagc tgcggcacgt
    10441 tccggcgctg ccttgtgtag ctcttcttca catttgatga agtactggcg aattgcgcgg
    10501 ccctgttcag tgcgttcgac catcgccagt tctttaccca tgttcacggt gagcaagtaa
    10561 tcatgagcaa gctgctggcg agattttgcg ctcgccggat ttggcgagct caaattttct
    10621 acaacctcaa aattcattcc ctttttaaat tcatactcct caatacgccc tttaatccaa
    10681 ttggtgaaat cacggcctac accgagcgct ttatgcagtg cgcgtgcgct gacaatgccc
    10741 gtttcatatc cgccaataat gccaggtgat accggaataa ttgcggcgaa atactgttga
    10801 ctcagttctt caggctgaac gaagccctgc ccctgtacgg gcatattcgg acgtttcatt
    10861 aaaaactcct gctgtagaat caggtttggt gctgacgggc gcggaactgt acgcccgtca
    10921 tgctgattac tgcttggtgt tgttgcggcg ctcgttcagc cagtggttga tctccaccgc
    10981 attaaatacg gtgtttttct cccccagctt cgtgggcttc ggtaatttgc cgatacgcac
    11041 ccagcggtcg atagttgaca cgctggtatc aagcaactta gccaggttgc agcggcggat
    11101 atagcccgtg gtagggatag cttcttgatg gttagctgtc atgattccct cttgggttaa
    11161 aagttgatgt tttctccatt ctattggttg tttgtacagc attccatcaa atctggaatc
    11221 tattaaaata ttcacaaatt ctattgaaat aatatgtttc cagtaaaaga ttgggtgagc
    11281 gggaagaggt gctaagactt tgaaagtttt tgctttattg gcgataagtc actgaatccg
    11341 tatacatcct gataccgtga tatacccact gcgtcgttat gcgctgttat ggtttgccat
    11401 cttttgccag tctttaacgt gctaatcctg tttatatcga tttataacga ttaataacgg
    11461 tgagtaaatt ttgctggtgg gggatgtatg gggattacag ccccgtgaat acagggcgtg
    11521 ttggctggtt aatctgcctg gcttactgcc agtaatgcgg ctgatatttt ttgcataaac
    11581 gtggatttgg cgatcccttt tggtgatatc cctttctcct tcatcaacgc ttccacgtcg
    11641 cggtaaattg ctgaacggtt tggctctccg tcctgaatgt atgcggttgt gcctgatttt
    11701 ttcacgagga gtaaaaccag taaccccatc atcttcatgt ttccctgctc ttcgtcgagt
    11761 ttcctgtgct gcccccttcc gctgcggctt gtttctttga tgtaacggac cagatcccga
    11821 ccgccaagat taagggcttt ttcctcccag ttttttgtcc cacgcaaatc atcgatagta
    11881 tctgttaccg ccttgattat tgcctctggc gtgcctgcct ccatgaacgg gaaggaagca
    11941 aggaatatat cccccgctct gtatggctca tccttgcttg tttttactcc actatgcagc
    12001 ctgatagcac gtgcgatatg agttctggct gattcgacct gttcgaactt gtcctgcggt
    12061 acgtcgccta tcctcattga cggattaaga tcagctaacg caagggccat ttcgtgggga
    12121 gtgattgaaa cagaagcgca aaggcgctct attggcgatt tcttcaggat gttaatcatg
    12181 atttagccct tctgattggt gtcacgttgt agtgttctcc gcgttccagc gacactaaca
    12241 gattggtcca cttcgccagt gcgtcccgtc gttcattgaa atactgatag cggttgtata
    12301 tgccttcgac tcccttgatg cggtgattga ggcatctttc agcgactacc ggatcaacac
    12361 caagcgcagc tagatgtgtg cgtgctgtac ggcggaagtc gtgaatagtg aaatttggaa
    12421 cttccggcat ctctgcgcgg acttttgcca gcgcaacagg tagggtgctc tcctgaatgt
    12481 gggggatcat cctgtgctgc attttccgcg ccggaagcac ccaggcacta ttgcatgaca
    12541 acacacgcaa ctctcgcagc cattcaataa cgggatggac gagtggaata tcgatggggt
    12601 cgccgttttt gctgcgttcc tccggcagat gccatacagc gttatcaagg tcgaattctt
    12661 cccatctggc ggcgcacaac tccatcttcc tgacacaaag cgccagcaat agcttaaatg
    12721 tgagttcatt ctcgcggctg aaaccctttg cggtccgcat agcctgaaag aatcggatta
    12781 gctcttcacg agtcagccaa cggtcgcggg aggactcctt gccaccagca tcagagactt
    12841 caaacgcaga gcatgggtta atctccagca catgacgttt taccccgtag tcgaatatgc
    12901 gacgcgtcca gcgcagaacg tccgttgcta tggttggcgc tccacggtca acaattgctt
    12961 ttagcatctc gtcaacgtgt cgtggtttta catcctcaac cttcatatga ccaatacgcg
    13021 ggtttatgtc cttgtcgatg cgacgacgga ggatatcggg atgtttccag cgcggcagga
    13081 tctggcgttc gaaatattcg gcggcaagct ctgatactcg tatggcgttt ttttctgcct
    13141 ccatttttgc cagtgcttcg gctttacgct cctgtttctc cgctgctacg tcgtacccaa
    13201 gtgcgacgcg ggcggatagt tcctttgctg tttctctggc tctggatagt gataactctg
    13261 catatgaccc gataaccatc gcccgttgtt tgccagcaaa cttatagcgg aatcgccaga
    13321 agggcattgt gtaatttttg ggatagcaga gatacaggcc gttaccgtca gctttgccgt
    13381 caaacctctc gtctgccttt atccaggcac ggatctgaat atctgtaagt tttggcatta
    13441 aggcatccct aaacattgaa tagaggtgtg tacataaagc gatgtacata aatggcatta
    13501 tgtacacctt tatgtacacg ctaatcatgc gctttcttgc gtcgttatga tagtcattaa
    13561 ttacaaaaaa aacccgcatt ttcgcgggtt tgattggatt attgcgcttt attgatttcc
    13621 tctgatagct tactccacat tttgaatctg ctcgcgcatc tgctcaatca acactttcag
    13681 ctcaatcgcg gagtttgtca cttcggcatt gatagacttc gaagcaagag tgttcgactc
    13741 gcggttgaac tcctgcatca taaagtccag acgacgacca acggcttctt ttttcttcag
    13801 aatgttgtac gtctctttaa catgcgcttc gaggcggtcc agttcttcgg caacgtcaat
    13861 tcgttgtgcc agcagaacca actcctgctc cagacggttg ttttccaact gcacctgagc
    13921 atcttccagc ttcgcgacca agcgttcacg ctgccattgc aggatttccg gcatatggga
    13981 gcggactttg accacttcgg cggtgacgcc ttccagacgc tgctcaatca acgctttcag
    14041 cgcctgacct tcggtttcgc gagcgatgat aaagtcatcc agggtgccat ccaacgccgc
    14101 gagaatttca gcggcaatcg cgtcgagatc ctgctcctgg gccgccatca cgcccggcca
    14161 acggagaata tcaactgggt tgatttctcc ttcgtcgctc tgcattttta cccagttcgc
    14221 ggcagttacc agctgtttag ccagtttttc gttgaggatc agctcacctt gcgcgctaac
    14281 atctggctca tagcgcaggg tacattccac tttaccgcgt gtcaggcgag aacgaatacg
    14341 ctcacgaacg acaggttcaa ggctacggaa ctgctccggc agacgaaagt aagtttccag
    14401 ataacgctgg tttaccgagc gcatttccca ggttgcgctc ccccattcac ccttgatttc
    14461 acgccgggcg taggcggtca tactgcggat catagacgtt cctgtttata aaaggagagg
    14521 tggaaggatt atagccatcg atgccttgtc aggataggaa taaccgccgg aagtccgtat
    14581 aatgcgcagc cacatttgtt tcaagccgga gatttcaata tgcgtccagc aggccgtagc
    14641 aataatcagg tgcgtcccgt taccctgact cgtaactata caaaacatgc agaaggctcg
    14701 gtgctggtcg aatttggcga taccaaagtg ttgtgtaccg cctctattga agaaggcgtg
    14761 ccgcgcttcc tgaaaggtca gggccagggc tggatcaccg cagagtacgg catgctgcca
    14821 cgttctaccc acacccgtaa cgctcgtgaa gcggcgaaag gaaagcaggg tggacgtaca
    14881 atggaaatcc agcgtctgat cgcccgtgcg cttcgcgcgg cagtagattt gaaagcgctg
    14941 ggtgagttca ccattacgct ggactgcgac gtgcttcagg ctgatggtgg cacgcgtacc
    15001 gcttcgatta cgggtgcctg cgtggcgctg gcagatgcgt tacagaagct ggtggaaaac
    15061 ggcaagctga aaaccaatcc aatgaaaggg atggtagccg cggtttctgt cggtattgtt
    15121 aacggcgaag caatttgcga tctggaatac gttgaagact cagccgcaga gaccgacatg
    15181 aacgtagtga tgaccgaaga cgggcgcatc attgaagtgc aggggacggc agaaggcgag
    15241 ccgttcaccc atgaagagtt actcaccttg ttggcgctgg cccgaggggg aatcgaatcc
    15301 attgtagcga cgcagaaggc ggcgctggca aactgatttt taaggcgact gatgagtcgc
    15361 cttttttttg tctgtagaaa agtaagatga ggagcgaagg catgaaacca tatcagcgcc
    15421 agtttattga atttgcgctt agcaagcagg tgttaaagtt tggtgagttt acgctgaaat
    15481 ccgggcgcaa aagcccctat ttcttcaacg ccgggctgtt taataccggg cgcgatctgg
    15541 cactgttagg ccgtttttac gctgaagcat tggtggattc cggcattgag ttcgatctgc
    15601 tgtttggccc tgcttacaaa gggatcccga ttgctaccac aaccgccgtg gcgctggcgg
    15661 agcatcatga cctggacctg ccgtactgct ttaaccgcaa agaggcaaaa gaccacggtg
    15721 aaggcggcaa tctggtcggt agcgcgttac aaggacgcgt aatgctggtg gatgatgtga
    15781 tcaccgccgg aacggcgatt cgcgagtcga tggaaattat tcaggccaat ggcgcgacgc
    15841 ttgctggcgt attgatttcg cttgatcgtc aggaacgcgg gcgcggcgag atttcggcga
    15901 ttcaggaagt tgagcgtgat tacaactgca aagtgatctc tatcatcacc ctgaaagatc
    15961 tgattgccta cctggaagag aagccggaaa tggcggaaca tctggcggcg gttaaggcct
    16021 atcgcgaaga gttcggcgtt taaagaaact cgccggatga aaagtcatcc ggcgtcatat
    16081 tactgcaact gcgcggcaat tagcggccag cgggcgtcaa aatcatccgt cgggcgatat
    16141 ttaaattcgc tgcggacaaa acgtgacagc ataccttcac agaaggccag caactggctt
    16201 gccagcagcg tttcatcagt ggtgtaacct tcaccctcac gcattctctt ttcacgcaat
    16261 acctgacgca gctgcgcttc aatacgctcg aaaagttggt tgatgcgccc ttgcaggcgg
    16321 tcttgttcaa acatcagcgc atgcccggta aggatgcggg tcaggccagg attacgctca
    16381 ccaaaaccga gaagcagcaa cactatcaga cgcaggcgcg ctgtggtgtc tttctcatct
    16441 ttcagaatca gattgatgcg agtaatcagg ctatcttcga taaactcaat caggctatcg
    16501 aacatgcggg tcttactggg gaagtggcga tacagtgccg cttcggaaac gccgacagag
    16561 gcggccagtt ttgccgtcgt gatacgttgg cttccatcgc tggattccag catcagcgcc
    16621 agagactgaa gtatttcctc gcgacggttc cttttcgcag tttgtttttc tgccatgtta
    16681 caaaataccc ctgaaaataa gcacttgcca ggcggcaccc acgctatgac cgcaaacgaa
    16741 atgtttgcgg ctatgttatg acgttattcg gatgcgtatg tgttactgac gaccagagtg
    16801 accaaagccg ccttcaccgc ggtcggtggc gtcgaaatct tccaccagat taaattcagc
    16861 ctgtactacc ggaacaaaaa tcatctgggc gatgcgttcg ccaggttgaa tggtgaagct
    16921 gtcctgacca cggttccaca cggaaatcat caactggccc tgatagtcgg aatcgatcaa
    16981 tcctaccagg ttaccaagca cgataccgtg cttatgtccc aatccggagc gcggcagcat
    17041 cattgccgcc agtgaaggat cggcaatatg aattgccagc ccggtcggaa ccagcgtagt
    17101 gtcacccgga gccagttcta cggcgtcgtc gagacaggca cgcaggtcaa gtccggcaga
    17161 gccagaggtg gcataagtcg ggagcggaaa ttccttccca acgcgcgggt ccagaatctt
    17221 aacgtcgatt tttttcatca taacgggtca cgatctcgtc gagtaataat tggccaagga
    17281 gctctttgcg ctcaagcggt aagactttat ctccgtcctg ccagaaaagg tgtaatgcgt
    17341 tgttgtcgct gttaaatcct tgagttggct gggaaacatc gttcgcgcag atcagatcaa
    17401 ggtttttacg gatacgtttt tgccgggcgt attcttccac attatttgtt tcggcggcaa
    17461 atccaacgac gtagggtcga tggtctttta gtgcggcaac gcctgcaacg atatcggggt
    17521 ttttaaccat ttttattgtt aattcatcac cctgcgtggc ctgctttttg attttttctg
    17581 gggccacggt agctgcgcga taatccgcca cggcggcgca gccgataaaa atattttgct
    17641 gctgtacaga agcattcacg gcggcttcca tttccagcgc ggtcatcaca tcaacacgtt
    17701 taacaaacgg tggcgtcggt agtgaaactg gacctgatac cagcgtgacg ttcgcgccac
    17761 gacgggcagc ggcggcggcg atagcaaaac ccatcttgcc ggagctgtga ttagagatat
    17821 aacgcaccgg atcgagcggt tcacgcgtcg ggccggcggt aatcataatg ttcagatgtt
    17881 tcaggtcgtt gacgggcgaa aaatgcgcta ctgccatatc cacaatggtt aacggatcga
    17941 gcattcgccc aggaccgata tcaccacaag cctgactgcc actgtctggc ccccagatga
    18001 gcaaaccacg ggaagcaagc acctctaaat tatgctgcgt ggcagcggca cggtacatct
    18061 gctggttcat ggcggggagc acggctacag gcgcaggtgt agccagacaa atcgtcgata
    18121 ccaggtcatt cgccattccg gcagcaacac gggcaatcaa atctgccgtg gcgggggcga
    18181 gaatcactaa atcagcccat ttacccagct caatatggcc catagcggct tcggctgccg
    18241 ggtcaagcag actgtcggaa acgggatacc cagaaaccgc ctgcaagcta agtggggtga
    18301 taaaggcttt tgccgcttcg gtcatggcta cgcggacgtc ggccccgcga tcgcgcaaac
    18361 gacgcaccag ttcaggggtt ttataggcag caataccgcc gctaacgccg agaacgattt
    18421 ttttaccggc caggctcatg atgatttttc ctgtagggtg acaccagaag ttggcgattt
    18481 tatcacaata cttttgttgt cgtgccttca cctgagattc actttgcgag gcgctttcca
    18541 ggattgaaaa ctgaccgtcg atttaacgga acggctatga caggatgcga gcaccacaaa
    18601 ggaggtgaag gtgaaaaaca atgcacagct gttgatgccg cgcgaaaaaa tgttgaagtt
    18661 tggtattagc gccttaacgg atgtcgagct gctggcgcta tttctgcgta ccggaacgcg
    18721 cggtaaagat gtattaaccc tggcaaaaga gatgctggag aatttcggct ctctttatgg
    18781 cttgttaacc tctgaatatg aacaatttag tggtgttcat ggaattggcg tagcgaaatt
    18841 tgcccagtta aaggggattg ctgaactggc gcggcgttac tacaacgtgc ggatgcgtga
    18901 agaaagccct ttactcagcc cggagatgac gcgggaattt ttacaaagcc agctcacggg
    18961 tgaggagcgg gagatcttta tggtgatctt tctcgactca caacaccggg ttataacgca
    19021 tagccgtctt ttttccggca cgctaaacca tgttgaagtc catcctcggg aaattatccg
    19081 cgaagcgata aaaataaacg cctcggcgct gatccttgca cataatcacc cttcgggttg
    19141 tgctgaaccc agtaaagcgg ataaactcat tactgaacgg ataataaaga gttgtcagtt
    19201 catggattta cgcgtgctcg accatatcgt gattgggcgt ggagagtatg tttcttttgc
    19261 cgaacgcggc tggatttaac ccgctatgcg cgatccttcg ggatctttgt ctgttcggga
    19321 cttgagcaca tcgctgagtc agcgtatact acgccacctt tgagaatctc gggtttggca
    19381 tttgggcctg gcaatcgaga gttcacatag aactgcgatg accgggctgt aaagcctgac
    19441 gaggcgccaa taccccatac gaagctcgag ctaatttgat ttttggagaa tagacatgtc
    19501 ccgagtctgc caagttactg gcaagcgtcc ggtgaccggt aacaaccgtt cccacgcact
    19561 gaacgcgact aaacgccgtt tcctgccgaa cctgcactct caccgtttct gggttgagag
    19621 cgagaagcgt tttgtcaccc tgcgcgtatc tgctaaaggt atgcgtgtaa tcgataaaaa
    19681 aggcatcgat acagttctgg ctgaactgcg tgcccgtggc gaaaagtact aagtacttag
    19741 aggaaataaa tcatggctaa aggtattcgt gagaaaatca agctggtttc ttctgctggt
    19801 actggtcact tctataccac tacgaagaac aaacgtacta agccggaaaa actggaactg
    19861 aaaaaattcg atccagttgt tcgccagcac gtgatctaca aagaagcgaa aatcaaataa
    19921 ttctcgcttt gatgtaacga aaaacctcgc tccggcgggg ttttttgtta tctgcttacc
    19981 cccatattga ttgcatctgt tcattcctgg agatgctatg cctgaattac ccgaagttga
    20041 aaccagccgc cgcggcatag aaccgcatct cgttggtgca accattcttc atgcggtggt
    20101 gcgcaacgga cgcttgcgct ggccggtttc agaagagatc taccgtttaa gcgaccaacc
    20161 agtgcttagc gtgcagcggc gggctaaata tctgctgctg gagctgcctg agggctggat
    20221 tatcattcat ttggggatgt ctggcagcct gcgcatcctt ccagaagaac ttccccctga
    20281 aaagcatgac catgtggatt tggtgatgag caacggtaaa gtgctgcgct acaccgatcc
    20341 gcgccgcttt ggtgcctggc tgtggaccaa agagctggaa gggcacaacg tgctggctca
    20401 tcttgggccg gagccgctta gcgacgattt caatggtgag tatctgcatc agaagtgcgc
    20461 gaagaaaaaa acggcgatta aaccgtggct gatggataac aagctggtgg taggcgtagg
    20521 gaatatctat gccagcgaat ctctgtttgc ggcggggatc catccggatc ggctggcgtc
    20581 atcactgtcg ctggcggagt gcgaattgct ggctcgggtg attaaagcgg tgttgctgcg
    20641 ttcgattgag cagggcggta caacgctgaa agattttctg caaagtgatg gtaaaccggg
    20701 ctatttcgcg caggaattgc aggtttacgg gcgaaaaggc gagccctgtc gggtgtgcgg
    20761 tacgccgatt gtggcgacta aacatgcgca gcgggcaacg ttttattgtc ggcagtgcca
    20821 gaagtaattc atgcgcgccg gatggcatac catccggcat aaacgctacg ctaacttcgc
    20881 catcagcgcc tgatggacat tctccggcag gaaatgggtg acatcgccct gatggcgcgc
    20941 cacctctttc accaacgatg aagagataaa cgaccactct ttcgacggca tcagaaacac
    21001 actttccagt tccggcatta agtggcgatt catatgcgcc agctgcattt catattcaaa
    21061 atccgccacc gcacgcaggc cacgaatcag caccgtagcg tgttgattac gggcgaagtt
    21121 cgccatcaaa tcactaaacc cgaccacttc cacgttcccc agatgcgcgg ttgcctgctg
    21181 tgccagctca acacgctctt ccagggtaaa catcggtttt ttactggggc tggcggcaat
    21241 cgccagaata acgtgatcga acatctgcgt ggcgcgcgtc acgatatcga tatgaccatt
    21301 ggtaatggga tcgaaagtac ccggataaat cgcccgtttt tgcataacaa cctcaatgcg
    21361 ttttcggtgg caggtaaggt tccagcagtt gaagcagacg ctgtagcgcg ccctggtttt
    21421 gatacagtac ttcaacggca tgacggccat agaaactacg gtaatcggcg tcggtgagta
    21481 aagaggaaac ctcttttgca agcgtagtgg catcggtaac ggtaatcagc ccacttgcct
    21541 gctccagccg cgcgcaaatg tctttaaagt taaaagtatg cggccccatc aataccggaa
    21601 ttgcgtgtgc ggcagcttcc agcggattat gcccaccacg ttcaaccagt gaaccgccaa
    21661 caaaggcgag atcggcaatg ccgtacagta acatcagctc gcccatcgta tcgccaacca
    21721 caacctgcgt gctggaggaa gggacttccc ctgaagagcg tgtgatatag cttagtccag
    21781 cctggcggac aaggttaatc gcatccggga agcgttccgg atgacggggt accaggatga
    21841 gcagtaaatt cgggaattgc tgtaacaatg cctgatgtgc ggcgatcacc acactctctt
    21901 cgccttcgtg agtgctggtg gcaatccata ccgggcggtg tggtgcccac tggcggcgca
    21961 gcgtcacggc tttagcagcc aactgcggcg ttacagaaat atcgaatttc aggctaccgg
    22021 taacggttac ttggttattt tttgcgccca gcgccacaaa acgtgcacca tcttcttcat
    22081 tttgggcggc aatcagcgta atacgacgca gcaagcgacg gacgaattta cccagtttgg
    22141 cataacctgc ggccgagcgg gcagagagtc gcgcgttagc gatcaccagc ggaattttac
    22201 gtttatgtag cgccgcaatc aggttaggcc atagttcggt ttccataatc aacaccagtt
    22261 tagggtcgac tttattcagg aaacggttga gtgcatcggg cagatcatac ggcagataaa
    22321 cgtgctgaac atccttcccg aaagccgatt gtacgcgctc cgaaccggtt ggcgtcatgg
    22381 ttgtaacggt aatcggtaaa tcaggataac gatgacgcag cgcgcgcacc aacgggatcg
    22441 ccgccagagt ttcaccgacg gagacggagt gcagcataat gccgcctggt tttagcggat
    22501 ggcggtaaaa accgtaacgt tcaccccagc gttttcgata ggccggagcc ttacgtccgc
    22561 gcacccagag ccgtatccag atcagcggct gaataaggta gagaagggcg gtgtaaagca
    22621 attcgagcat agtaaatagc tgacttatgg atgtgctggg gattctatgt atttagctgt
    22681 ggctttacca ttacttttcc cgtttttgac ttaaatagca atttacccgc caattcttgc
    22741 tgaacatcta tacttttttt gtatttttat gcgattcatt gatttctgct gtacgtgcag
    22801 gaaggaatcg cagagcaaca gtgaaagaat atggttgcta atgattttat ttgattgatt
    22861 tctttattga aaatcaacaa tgtgcttaaa aatcattcgg gtcggattgc cgcgaaagaa
    22921 gtgatacact agcgcgtcaa tatcagtgcg gtatcagtat tcaggtagct gttgagcctg
    22981 gggcggtagc gtgctttttc tgcttcacct taatcggata atctcaacaa aaagagttac
    23041 ttgtggataa gccatttcga agaattttgc tcattaagat gcgttttcat ggggatatgt
    23101 tattaactac tcccgtcatt agttcgctga aaaaaaatta ccctgacgca aaaatcgatg
    23161 tgctgcttta tcaggacacc atcccgatcc tgtctgaaaa tccagagatt aacgcgctct
    23221 acggcataaa aaataaaaaa gcaaaagcct cagaaaaaat tgccaacttt tttcatctca
    23281 tcaaggtatt acgtgccaat aagtatgacc ttatcgtcaa tcttaccgat caatggatgg
    23341 ttgctatact ggttcgctta ttaaatgccc gtgtgaaaat ttcccaggat tatcatcatc
    23401 ggcagtctgc tttttggcgt aaaagtttca cccatttggt gccgttgcag ggtggaaatg
    23461 tggtggaaag taacttatcc gtgctgacat cattgggact tgattcgttg gtgaagcaga
    23521 caaccatgag ttacccgcct gcaagctgga aacgtatgcg tcgcgaactt gatcacgctg
    23581 gtgttggaca aaattatgtg gttatccaac ctacggcgcg gcaaatcttc aaatgctggg
    23641 acaacgccaa gttttccgct gtgattgatg ccttacatgc tcgtggttat gaagtcgttc
    23701 tgacgtccgg cccagataaa gacgatctgg cctgcgtcaa tgaaattgcg cagggatgcc
    23761 agacgccacc agtaacggcg ctggctggaa aggtgacctt cccggaactt ggtgcgttaa
    23821 tcgatcatgc gcagctgttt attggcgttg attccgcacc ggcgcatatt gccgctgcag
    23881 ttaatacgcc gctgatatcg ctgtttggtg cgacagacca tattttctgg cgtccctggt
    23941 caaataacat gattcaattc tgggcgggag attaccggga aatgccaacg cgcgatcagc
    24001 gtgaccgaaa tgagatgtat ctttcggtta ttccggcggc agatgtcatt gctgctgtcg
    24061 ataaattatt gccctcctcc acgacaggta cgtcgttatg atcgttgctt tttgtttata
    24121 taaatatttt ccctttggcg gtttgcagcg cgattttatg cgtattgctc agacagtcgc
    24181 cgcccgaggt catcatgttc gggtttatac ccagtcgtgg gaaggcgaat gccctgatgt
    24241 atttgaactg atcaaagtgc cggttaaatc gcataccaat cacgggcgca atgcggagta
    24301 ttttgcctgg gtgcaaaaac atttacgcga acatcccgtc gataaagtcg ttggattcaa
    24361 caaaatgccg gggctggacg tttattatgc cgctgatgtt tgttatgccg agaaagtagc
    24421 gcaggaaaaa ggctttttct atcgcctgac gtcacgttat cgccattatg ccgcctttga
    24481 gcgggcaacc ttcgaacagg gcaagccgac acagctgctg atgctgacag ataagcaaat
    24541 cgccgatttc cagaaacatt atcagactga agcggagcgt tttcatattc tgccaccggg
    24601 gatttatcct gatcgtaaat atagccagca gccagccaat agccgtgaaa tcttccgtaa
    24661 gaagaatgga ataaccgaac aacaatattt attgttgcag gtcggttcag acttcacgcg
    24721 taaaggtgtc gatcgttcca ttgaagcact tgcttcgtta ccggattcgc tgcgccacaa
    24781 cacattgcta tatgttgttg ggcaggataa accgcgaaaa tttgaggcgc tggcagaaaa
    24841 acgcggcgtg cgcagtaatg ttcacttctt ctcggggcgc aacgatgtct cggaattaat
    24901 ggcggcggcg gatttattac tgcatcctgc ctaccaggaa gcggcgggaa ttgtgctgct
    24961 ggaagcgatt actgcaggat taccggtact aacaacagcc gtttgtggct atgcgcatta
    25021 tattgtcgag gccaattgcg gcgaggctat tgttgagcca ttccgccagg aaacattgaa
    25081 tgagatttta cgcaaagcgt taacgcaatc ttcattgcgc caggcctggg cggaaaatgc
    25141 gcgacattat gctgatacac aagatttata cagtctgcca gagaaagcgg cggatatcat
    25201 aacgggtggt ctggatggtt gaacttaaag agccgtttgc cacgttatgg cgcggtaaag
    25261 atccttttga ggaagttaaa accttgcagg gtgaggtatt tcgtgaactg gaaactcgcc
    25321 gtactctgcg ctttgaaatg gcgggcaaaa gctattttct caaatggcat cgcggcacga
    25381 ccctgaaaga gataatcaaa aatttactct cattgcggat gccagtatta ggcgcagacc
    25441 gcgaatggaa tgcgattcat cgactgcggg atgtcggcgt tgatactatg tatggggtgg
    25501 catttggcga aaaaggcatg aatccgctga ccagaacttc atttattatt accgaagatc
    25561 tgacaccaac cataagtctg gaagattact gtgctgactg ggcgactaac ccttcagatg
    25621 ttcgcgtaaa gcgtatgctt attaagcgtg tcgcgacgat ggtgcgcaat atgcatgctg
    25681 tgggcattaa ccaccgtgac tgttatatct gtcatttcct gctgcacttg cctttttccg
    25741 gtaaggaaga ggagttaaaa atttcggtaa ttgacctgca ccgggcgcag cttcgcacgc
    25801 gcgttccacg tcgttggcgc gataaagatc ttattgggct ttatttttca tcgatgaata
    25861 tcggcctgac tcagcgggat atctggcggt ttatgaaagt gtattttgcc gccccgctta
    25921 aagacattct caagcaggaa caaggactgc tgtcgcaagc agaagcaaaa gccacaaaaa
    25981 tcagggaaag aacgattcga aaatcgttgt aacccgtaac ttatttttgc caaaattttg
    26041 gatacagaat aaat
//
LOCUS       DAZKFU020000043        25898 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZKFU020000043 DAZKFU020000000
VERSION     DAZKFU020000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.043"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..996
                     /gene="robA"
                     /locus_tag="ACHOQQ_RS07080"
                     /old_locus_tag="pgaptmp_001416"
     CDS             127..996
                     /gene="robA"
                     /locus_tag="ACHOQQ_RS07080"
                     /old_locus_tag="pgaptmp_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGU4469686.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(993..1640)
                     /gene="gpmB"
                     /locus_tag="ACHOQQ_RS07085"
                     /old_locus_tag="pgaptmp_001417"
     CDS             complement(993..1640)
                     /gene="gpmB"
                     /locus_tag="ACHOQQ_RS07085"
                     /old_locus_tag="pgaptmp_001417"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGU4469687.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            1692..2204
                     /gene="yjjX"
                     /locus_tag="ACHOQQ_RS07090"
                     /old_locus_tag="pgaptmp_001418"
     CDS             1692..2204
                     /gene="yjjX"
                     /locus_tag="ACHOQQ_RS07090"
                     /old_locus_tag="pgaptmp_001418"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGU4469688.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(2351..2677)
                     /gene="trpR"
                     /locus_tag="ACHOQQ_RS07095"
                     /old_locus_tag="pgaptmp_001419"
     CDS             complement(2351..2677)
                     /gene="trpR"
                     /locus_tag="ACHOQQ_RS07095"
                     /old_locus_tag="pgaptmp_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGU4469689.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(2767..4704)
                     /gene="sltY"
                     /locus_tag="ACHOQQ_RS07100"
                     /old_locus_tag="pgaptmp_001420"
     CDS             complement(2767..4704)
                     /gene="sltY"
                     /locus_tag="ACHOQQ_RS07100"
                     /old_locus_tag="pgaptmp_001420"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGU4469690.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            4915..6582
                     /gene="ettA"
                     /locus_tag="ACHOQQ_RS07105"
                     /old_locus_tag="pgaptmp_001421"
     CDS             4915..6582
                     /gene="ettA"
                     /locus_tag="ACHOQQ_RS07105"
                     /old_locus_tag="pgaptmp_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGU4469691.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(6689..6856)
                     /gene="nadS"
                     /locus_tag="ACHOQQ_RS07110"
                     /old_locus_tag="pgaptmp_001422"
     CDS             complement(6689..6856)
                     /gene="nadS"
                     /locus_tag="ACHOQQ_RS07110"
                     /old_locus_tag="pgaptmp_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGU4469692.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(6890..8122)
                     /gene="nadR"
                     /locus_tag="ACHOQQ_RS07115"
                     /old_locus_tag="pgaptmp_001423"
     CDS             complement(6890..8122)
                     /gene="nadR"
                     /locus_tag="ACHOQQ_RS07115"
                     /old_locus_tag="pgaptmp_001423"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGU4469693.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(8143..9525)
                     /gene="radA"
                     /locus_tag="ACHOQQ_RS07120"
                     /old_locus_tag="pgaptmp_001424"
     CDS             complement(8143..9525)
                     /gene="radA"
                     /locus_tag="ACHOQQ_RS07120"
                     /old_locus_tag="pgaptmp_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGU4469694.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(9574..10542)
                     /gene="serB"
                     /locus_tag="ACHOQQ_RS07125"
                     /old_locus_tag="pgaptmp_001425"
     CDS             complement(9574..10542)
                     /gene="serB"
                     /locus_tag="ACHOQQ_RS07125"
                     /old_locus_tag="pgaptmp_001425"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGU4469695.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            10648..11292
                     /locus_tag="ACHOQQ_RS07130"
                     /old_locus_tag="pgaptmp_001426"
     CDS             10648..11292
                     /locus_tag="ACHOQQ_RS07130"
                     /old_locus_tag="pgaptmp_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HGU4469696.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            11320..12336
                     /gene="lplA"
                     /locus_tag="ACHOQQ_RS07135"
                     /old_locus_tag="pgaptmp_001427"
     CDS             11320..12336
                     /gene="lplA"
                     /locus_tag="ACHOQQ_RS07135"
                     /old_locus_tag="pgaptmp_001427"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGU4469697.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            12368..12631
                     /locus_tag="ACHOQQ_RS07140"
                     /old_locus_tag="pgaptmp_001428"
     CDS             12368..12631
                     /locus_tag="ACHOQQ_RS07140"
                     /old_locus_tag="pgaptmp_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4469698.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(12715..13434)
                     /gene="deoD"
                     /locus_tag="ACHOQQ_RS07145"
                     /old_locus_tag="pgaptmp_001429"
     CDS             complement(12715..13434)
                     /gene="deoD"
                     /locus_tag="ACHOQQ_RS07145"
                     /old_locus_tag="pgaptmp_001429"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGU4469699.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(13491..14714)
                     /gene="deoB"
                     /locus_tag="ACHOQQ_RS07150"
                     /old_locus_tag="pgaptmp_001430"
     CDS             complement(13491..14714)
                     /gene="deoB"
                     /locus_tag="ACHOQQ_RS07150"
                     /old_locus_tag="pgaptmp_001430"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4469700.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(14766..16088)
                     /gene="deoA"
                     /locus_tag="ACHOQQ_RS07155"
                     /old_locus_tag="pgaptmp_001431"
     CDS             complement(14766..16088)
                     /gene="deoA"
                     /locus_tag="ACHOQQ_RS07155"
                     /old_locus_tag="pgaptmp_001431"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGU4469701.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(16215..16994)
                     /gene="deoC"
                     /locus_tag="ACHOQQ_RS07160"
                     /old_locus_tag="pgaptmp_001432"
     CDS             complement(16215..16994)
                     /gene="deoC"
                     /locus_tag="ACHOQQ_RS07160"
                     /old_locus_tag="pgaptmp_001432"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGU4469702.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            17252..18802
                     /locus_tag="ACHOQQ_RS07165"
                     /old_locus_tag="pgaptmp_001433"
     CDS             17252..18802
                     /locus_tag="ACHOQQ_RS07165"
                     /old_locus_tag="pgaptmp_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HGU4469703.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            18774..19637
                     /locus_tag="ACHOQQ_RS07170"
                     /old_locus_tag="pgaptmp_001434"
     CDS             18774..19637
                     /locus_tag="ACHOQQ_RS07170"
                     /old_locus_tag="pgaptmp_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGU4469704.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVEEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(20034..20816)
                     /locus_tag="ACHOQQ_RS07175"
                     /old_locus_tag="pgaptmp_001435"
     CDS             complement(20034..20816)
                     /locus_tag="ACHOQQ_RS07175"
                     /old_locus_tag="pgaptmp_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4469705.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEHAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(20813..21886)
                     /locus_tag="ACHOQQ_RS07180"
                     /old_locus_tag="pgaptmp_001436"
     CDS             complement(20813..21886)
                     /locus_tag="ACHOQQ_RS07180"
                     /old_locus_tag="pgaptmp_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGU4469706.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(22008..22169)
                     /locus_tag="ACHOQQ_RS07185"
                     /old_locus_tag="pgaptmp_001437"
     CDS             complement(22008..22169)
                     /locus_tag="ACHOQQ_RS07185"
                     /old_locus_tag="pgaptmp_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGU4469707.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(22296..22901)
                     /gene="osmY"
                     /locus_tag="ACHOQQ_RS07190"
                     /old_locus_tag="pgaptmp_001438"
     CDS             complement(22296..22901)
                     /gene="osmY"
                     /locus_tag="ACHOQQ_RS07190"
                     /old_locus_tag="pgaptmp_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGU4469708.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(23294..24880)
                     /gene="prfC"
                     /locus_tag="ACHOQQ_RS07195"
                     /old_locus_tag="pgaptmp_001439"
     CDS             complement(23294..24880)
                     /gene="prfC"
                     /locus_tag="ACHOQQ_RS07195"
                     /old_locus_tag="pgaptmp_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGU4469709.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVA
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            25100..25348
                     /locus_tag="ACHOQQ_RS07200"
                     /old_locus_tag="pgaptmp_001440"
     CDS             25100..25348
                     /locus_tag="ACHOQQ_RS07200"
                     /old_locus_tag="pgaptmp_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4469710.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            25775..25888
                     /locus_tag="ACHOQQ_RS07205"
                     /old_locus_tag="pgaptmp_001441"
     CDS             25775..25888
                     /locus_tag="ACHOQQ_RS07205"
                     /old_locus_tag="pgaptmp_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HGU4469711.1"
                     /translation="MNSILIITSLLIIFSIFGHALIKLGIGISNNPDKTDV"
BASE COUNT         6066 a         7109 c         6637 g         6086 t
ORIGIN      
        1 ttatttccgt caagacctaa aacatactct actaagaaaa aaacactgaa tgctaaaaca
       61 gcaaaaaatg ctattatcca attacctgat gtcaggtgct cgttgttgaa aggatgagga
      121 tattttatgg atcaggccgg cattattcgc gaccttttaa tctggctgga aggtcatctg
      181 gatcagcccc tgtcgctcga caatgtagcg gcgaaagcag gttattccaa gtggcactta
      241 cagagaatgt ttaaagatgt cactggccat gctattggcg cgtatattcg tgctcgtcgt
      301 ttgtcgaaat cggcggtcgc actacgcctg actgcgcgtc cgattctgga catcgcgctg
      361 caataccgct tcgactctca acagacattt acccgcgcat tcaagaagca gtttgcccag
      421 actcctgcac tttaccgccg ttctcctgaa tggagcgcct ttggtattcg cccgccgctg
      481 cgtctgggtg aattcaccat gccagagcac aaatttgtca ccctggaaga tacgccgctg
      541 attggtgtta cccagagcta ctcctgttcg ctggagcaaa tctctgattt ccgccatgaa
      601 atgcgttatc agttctggca cgattttctc ggcaacgcgc cgaccattcc gccagtgctc
      661 tacggcctga atgaaacgcg tccgagtcag gataaagacg acgaacaaga ggtattctat
      721 accaccgcgt tagcccagga tcaggcagat ggctatgtac tgacggggca tccggtgatg
      781 ctgcagggcg gcgaatatgt gatgtttacc tatgaaggtc tgggaaccgg cgtgcaggag
      841 tttatcctga cggtatacgg aacgtgcatg ccaatgctca acctgacgcg ccgtaaaggt
      901 caggatattg agcgatacta cccggcagaa gacgccaaag cgggagatcg cccaattaat
      961 ctgcgctgtg aactgctgat tcctatccgc cgttaacgct gcagctcatc taatgcaggg
     1021 gcgtccagat gcgagatgtc ccctgcggtt tcaacgaccc agccagacgc cagccacagg
     1081 ctttcctgat aatccacgcg cgaaatagaa cagttacgca gacgtaagcg gcgctctgcc
     1141 catgctggta atccgagaat cgtactcacc agacatccca gtgcaatacc atggctcacc
     1201 agcagcggtc ggcttccctg cggtaagtcc cggcaagatt ccagtgccgc attgacgcga
     1261 tcgctgagct cctgcattga ctcgccttca ggaatacgcc catcaacggt gccattgacc
     1321 agctgccgac gccagttctc ttcttcttcg gtcagagaat cgatatgtct tttttccagc
     1381 acacccatgt ttaattcacg caggcgagaa tcaaagatga tgtcacagcc gcaggcctgg
     1441 gcgatgattt ccgccgtacg ccgggtgcgt cctaaatcgc tactgatgat atgcgtaatg
     1501 ccaagctctt tggcacgggt tgccacctgc atcgcctgtt gctcaccttt ggcggtcagc
     1561 gggctgtcag actggccctg aatacgtcgc tcggcgttcc actgcgtttc accgtggcgg
     1621 actaggtata cctgtaacat gctttttttc cgtatactgc tgtaacgtta attttctgag
     1681 taatactgat tatgcaccaa gttgtctgtg cgaccaccaa tcccgctaaa attcaggcca
     1741 ttctgcaggc atttcacgag atcttcggcg aaggatcctg ccatattgca tccgttgccg
     1801 tcgagagcgg tgtaccggaa cagccctttg gcagtgagga aacgcgcgct ggcgcacgaa
     1861 atcgggtagc aaatgcccgc cgtttacttc cagaggctga tttttgggtg gcgattgaag
     1921 ctggcattga tggcgacagc actttcagtt gggtggtgat tgaaaacgcc agccagcgcg
     1981 gcgaggcgcg ttcggctact ttgccgttgc ctgcggtgat tctggaaaaa gtgcgtgaag
     2041 gtgaagcgct tggtcccgtg atgtcgcgtt ataccggtat tgatgagatt ggtcgtaagg
     2101 aaggggcgat cggcgtgttt accgccggga aactcactcg cgccagcgtc tatcaccagg
     2161 cggtgatcct ggcgctgagt ccgtttcata atgccgtgta ttaagcgcct gatgcgacgc
     2221 tgccgcgtct tatcatgcct accaaacata ttgaaattac gggtatttgt aggacggata
     2281 aggcgttcac gccgcatccg gcattcggtg cacgatgcct gatgcgccac gtcttatcag
     2341 gcctacaaaa tcaatcgctt ttcagcaaca cctcttccag ccactggcgc agctcgacgg
     2401 gcgcggcttt caggctgtta gatccacgcg taatcgtcgc gatgcctgcg ccgagttcat
     2461 tttttaactc acgctggctc atttcgccgc gcaacagctc ttcgacaata cgcacgcgag
     2521 tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat
     2581 cgttttggta ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt
     2641 ctgccatcgc tgctgaatag ggtgattgtt gggccataat atgtcgccat tgttagcggg
     2701 ggaagcaaaa tgcctccccc ggttgtactc gctaaagagt acgatagcat atcataaacg
     2761 tgtggatcag taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat
     2821 gaaatagcgg tagtaagcgt cataagccag cacgttcttc acataaccgc gcgtctcgga
     2881 gaatggaata ctctcgacaa atgccactgc gtcgatacgc ccggcgctgt tgccaagcca
     2941 ggttcgcacc cgccctggtc cggcgttata agctgctgag gagaaaatac gattattgcc
     3001 aaattgctga taaacatatt gcaggtaact ggtgccaatg ttgatattcg tttccggatc
     3061 cagcaattgc ccagggctgc tataaccagg aatagagaac atcttcaccg tatgggtcgc
     3121 cgtgcccggc ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt
     3181 ccaggcgctc tcctgacggg caatcgccat cgcatagctt tgcgggatct ccttaccgct
     3241 ggtgtagcgt ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca
     3301 caacttcccg gcgatcgttg cctgaacgct aagatcccac cattggttgt tgaaagcata
     3361 ccgcgccagt tgagcttgct ctgtttttga cttgctcttc accagattgg cccactcgct
     3421 acgcgcggtg ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc
     3481 ctgagtcagg gcgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc
     3541 tccgatgcgt tgcgctgcaa ccatcgggta gaaaccacgc tgttgcatga gttgatgcaa
     3601 aatctctttt gcttcagctt cacgtccgcg ttccagcaat aaatccgcct gccagtaacg
     3661 ccattcatct ttctctttcg cctccatcgg caaacgcgcc agccaggtat tcaggccgcg
     3721 acgatcgcca gtgccaagcg ccattcgcac acggcgttca atcaacgacg tggattgcga
     3781 gcgcataatg gcgtcatcgc gccatttcgc ctgctcgtcg gtgacatcgt tgcccatcaa
     3841 acgccaggcg acgatatcgc gcagctcctg aatctgatct tcattaagct gctgcgcctg
     3901 ggcaagcgac gggatcatca gccgtgcatt ttcagcatcc tgccgcgcca cactggcaaa
     3961 cgccaccgcc gccatttgac gggtaaaatc ggtcgctccg gttgtacgcg cgaaggtcag
     4021 taccgtatta gggttgttcg ccagtgaaat gattgccgag gcgatagtct ggtaatcggc
     4081 aggcatctgc cctgccagca ctgttaccag gccagtgtta cccgctttca tcgccagacg
     4141 gatacgctct aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa
     4201 cttgtcacag gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca
     4261 ggcttcttca ctctgcccgg tgttccattt cgcatagtag taattacatt gcgcttcggt
     4321 agttccgggc ttttccgggc taaaggctaa caagccacgc cagtcttcac gccgcgccag
     4381 ttcattgacg aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac
     4441 aaagttagtg accgtcaccg ccggttgatt catcaggtca tcggtgatct ggcggtattc
     4501 caggtaggga taaagcggat aatccttcag tccaggcatc atttgttcga ccacatccat
     4561 ttgtcgatta tcccaggcct gcttgatttg cgcgtaacga ctacgctgct catccagtga
     4621 gtcggctcgc gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca
     4681 ggtaacttgt ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc
     4741 gtcatgccgc gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac
     4801 acttttttac ttttactgcg aaggtgatgg accaactggg attaggcggg gaaaaaggct
     4861 acacttcgcc tttgaaaact cattatcacg ataaacatag aggcgaagtc caacgtggct
     4921 caattcgttt ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg
     4981 aaaaacatct ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc
     5041 gcgggtaagt ccaccctgct gcgcattatg gcaggcattg ataaagacat cgaaggtgaa
     5101 gcgcgtccgc agccagacat caagattggt tatctgccgc aggaaccgca gctgaacccg
     5161 gaacacaccg tgcgtgagtc cattgaagaa gcggtttcag aagtggttaa cgccctgaaa
     5221 cgcctggatg aagtgtatgc gctgtacgcc gatccggatg ccgattttga caagctggcc
     5281 gctgaacaag gccgtctgga agagatcatt caggctcacg acggtcataa tctgaacgta
     5341 cagctggagc gtgcggcgga tgcgctacgt ctgccggact gggacgcgaa aatcgctaac
     5401 ctctccggtg gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga aaaaccagac
     5461 atgctgctgc tcgacgaacc gaccaaccac ctggatgccg aatccgtggc gtggctggaa
     5521 cgcttcctgc acgacttcga aggcaccgtg gtggcgatta cccacgaccg ttacttcctc
     5581 gataacgttg cgggctggat cctcgaactt gaccgcggtg aaggtattcc gtgggaaggt
     5641 aactactcct cctggctgga gcagaaagat cagcgtctgg cgcaggaagc ttcacaagaa
     5701 gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc
     5761 cgccagtcga aaggtaaagc acgtctggca cgctttgaag agctgaacag caccgaatac
     5821 cagaaacgta acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa
     5881 gtgctggaag tcagcaacct gcgtaaatcc tacggcgatc gcctgctgat tgatgacctg
     5941 agcttctcga tcccgaaagg ggcgatcgtc gggatcatcg gtccgaacgg cgcgggtaaa
     6001 tcgaccttgt tccgtatgat ctccggtcag gaacagccgg acagcggcac catcactttg
     6061 ggtgaaacgg tgaaactggc atcggttgat cagttccgtg actcaatgga taacagcaaa
     6121 accgtttggg aagaagtttc cggcgggctg gatatcatga agattggcaa caccgagatg
     6181 ccaagccgcg cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgcgtt
     6241 ggtgaactct ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc
     6301 ggcaacatgc tgctgctcga cgaaccaact aacgacctgg atatcgaaac cctgcgcgca
     6361 ctggaaaacg ccctgctgga gttccctggc tgcgcgatgg ttatctcgca cgaccgttgg
     6421 ttcctcgacc gtattgccac gcacatcctg gactaccagg atgaaggtaa agttgagttc
     6481 ttcgaaggta actttaccga gtacgaagag tacaagaaac gcacgctggg cgcagacgcg
     6541 ctggagccga agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg
     6601 cggcgcgaac gccttatccg gcctacaaaa ccagcaattt caatacgttg taaaactgta
     6661 ggcctgataa gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc cggatgcggc
     6721 gtgaacacct tttccggtct acaacagcct tagcatcctc aatttcgtgg cgggtcaccc
     6781 gtgcaggttt tttcaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact
     6841 catcaaaaaa actcatttta tacctcgcct ttgagcagtt tcatcacggt tatctctgct
     6901 cccccatcat ctcccgcacc agctccacgc agcgcaggaa acggctgtcg taatcttcct
     6961 cttcaacccg cacaaattcg atattgttct cttccagcat ctccaccagc agattctgga
     7021 actctttacg atccaccgaa ctgccgaggc tgcgtaaacc atccgccacc cacggcgtgt
     7081 tgttctccag caggatcacc agatcgaaac ggtattcatc aatcagcgcc tgcacgaacg
     7141 gatgttcccg cccttcgtac tttttgcaga acgcctgagt ggtgacaaaa tcggtatcga
     7201 taaatgccac tttattggca tatttcaccg caaaatcaat gtattgagcg tggcccagcg
     7261 cgattttatc gtagtcagaa tactgcaatg cgatctcatc accgccgagg tgtgaaaaga
     7321 cataatcgcg gccatattcc cacgcactgg tggtgttgaa gatattggca agtttgttta
     7381 ccagggtgga tttaccgctc gactcgccgc caaggatcgc cacggtacgc acaaaaaacg
     7441 gcttcacttc ggtaggaata tattcccagt agcggaacgg gttttcgcgg atctgcgcac
     7501 cgctgatact cataaaggta cgtttcggat cgaccagcac cgtctcgatc cccagatgtt
     7561 ccatatactg tggcgcatcg gcttcttccg aggtgtagat cagatccggc tggatccctt
     7621 tttcagccat aaactttttg ataccgttgc tccacacatc ccagccgtgc ggatacggct
     7681 ccatgccctc ttcgttgaaa gcatgaatgc gaatattttt ctgatattta aaagtttgca
     7741 ataaccagcg cagacgatcc ggcacggtcg gctgctgcga catggcactg tcttcgaaca
     7801 acgcgcggtc acgggtatcg tcaaaaccca taatgatatg cagctcgtca acctggctac
     7861 aggcgcgctg gataaggtag atatgtccgg tatgcagtgg gtagaactta ccgaatacga
     7921 caccgatcgt tttcttctgc cggggaaact caagccccaa aaaacggtgc aacgcctcca
     7981 gcttttgcgc gctggggctt ttgattttgg cattcagtaa ctggcttaaa taccctttgg
     8041 tcataccgct ggcatcagct acctgctgta gcgtgcagcc ctgttgcttg atggcagttt
     8101 tcaggtaatc aaatgacgac atatctccct ccgtatttct cattataagt cgtcgaacac
     8161 gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc atcccttccg gcgctttttt
     8221 cggtacgtta gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg cttcagagat
     8281 tcgttcctga ccgctgggca ccgggcggat ctcccctgcc agcccgactt caccaaacac
     8341 caccagatcc tgcggtagcg gtctgtcacg caggctggaa accatcgcca gcagtaacgc
     8401 taagtcggcg ctggtttcgg ttacctttac gccgccgacc acgttcacaa acacatcctg
     8461 atcggccatt tgcagaccac cgtgacggtg caagacagcc agcaggattg ccagacggtt
     8521 ttgttccagc cccactgcca cgcggcgcgg attcgccatc atcgagtgat cgaccagcgc
     8581 ctgaatctcc accagcaacg gacgcgttcc ttcccacacc accatcacgg agctgccgga
     8641 ggtcacttca tctccgcgac ttaagaaaat tgccgaaggg ttgctgactt cacgcagccc
     8701 ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt ttttatggct
     8761 gcgcaaggtg cgaaaacggg agtcggcatc gccatccaaa agcaccgaac agtcgataca
     8821 gtgttccagc actttcgggc cagccagcga accatctttg gttacgtgcc ccaccatgac
     8881 aatcgccaca ccgcgcgttt tggcgaagcg tgtcaaataa gccgccgttt cacgcacctg
     8941 cgccacgcta ccaggcgatg actgtacatc cgccatatgc atcacctgga tcgagtcaat
     9001 taccatcagc ttcggttgct cttcttcggc aatcaggcag atctgttcga tgctggtttc
     9061 cgacaacata ttgagattgt cagtcggcag gccaaggcga tgagcgcgca ttgccacctg
     9121 ttgcagcgac tcttcgccgg tgacatacag cgttttcatc tgctgggcca gtttgcacag
     9181 cgtttgcagt agcagcgtgg atttccccgc accagggtta ccgccaatca gaatggcact
     9241 tcctggcacc acgccgccgc ctagtacgcg gtcgaactct ttaaatccgg tggaaaaacg
     9301 cggcagctct tcaaggctga tatcggagag tttctggact tttgccaccc cggcgctacc
     9361 ggcatagccg ctgagacgct cgttacgcgc caccgtaggc gacgcagcaa gacgcacctc
     9421 ggtgatggtg ttccaggcat gacaggcact gcactgcccc tgccagcgcg gataatcggc
     9481 cccgcattca ttacaaacaa aggcgcgttt tggagctttt gccacggtgt acctcgttaa
     9541 tgctgtgccg cccgcaggat ggcgggcgag caattacttc tgattcaggc tgcctgagag
     9601 gatgcagaat acccccatca ggtcagcgtg acggatggtg acttccgcct tttcattcac
     9661 ttttggcttg gcatggtagg caatccccag ccctgccgct ttgatcatcg gcaggtcatt
     9721 ggctccatcg ccaatcgcca cggtctgcgc cagcgggatt tcatactcct gcgcgaggcg
     9781 agtcagagtt ttcgctttgt actgcgcgtc tacgatgtcg ccgatcacat tgccggtaaa
     9841 tttaccgtcc atgatctcca gttcattggc taccacggct gtcaggcgca gcttgtcgcg
     9901 caggtattca gcaaagaaag taaagccgcc ggaggcaatc gccactttcc agcccagcgt
     9961 ttccagcttg agcaccagtt gcgttaagcc tggcatcagc ggcagatttt cacgcacctg
    10021 ttgcagaata ttggcgtcag cgcctttcag cgtcgccacg cggctgcgca ggctggcggt
    10081 aaaatcgagt tcgccgcgca tcgcccgttc ggttacttcc gccaccatct cgcccgttcc
    10141 ggccagtttg gcaatttcat caatacattc aatctggatg gcggtggaat ccatgtccat
    10201 caccagcaaa cccggcgtgc gcaggtgcgg gatttttccc agcggcgcga catccagctg
    10261 cgcttcgtgg gccaggcgtg tagcccgtgc ggtgagtgaa cctgccagac gaatcacctg
    10321 ataatcttcc acgcaccagg cggcaacaat caccatcgcc gcacccagtt tgctctggta
    10381 ttgggtcaga cgctgtttat ccagcccacg accatacagc agccagccgc tacgacctgc
    10441 gtggtaatcc agtggcatca cttcatcacc acttaatgaa agaggcagac ccggccataa
    10501 agagacatct tcaggcaggt cgcaccaggt aatgttaggc attaaggctc ctgtaaaatc
    10561 gttcgaagca gggaaaataa cgcatgaggc taccttgtat ccattgcttc tggcaacatt
    10621 aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt ccggctacat
    10681 cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg cactgatgca gggggcgtca
    10741 tggtttagtc aaaaccacca gcgacagcgt aatccacagc tggaagaact ggcccgcacc
    10801 ctggcgcgtc aggtgacgct gaacgttgca ccgctgatgc gtaccgactc accggatgaa
    10861 aaacgcattc aggcgatcct cgatcagtta acggatgaaa gccgtatcct cgacgcgggt
    10921 gtgtatgacg aacaaggcga tcttatcgcc cgttctggcg aaagcgtcga agtgcgcgac
    10981 cggctggcgc tcgacggcaa aaaagcgggc ggctatttta accagcagat tgtcgaaccc
    11041 attgccggga aaaacggacc gctcggctat ctgcgcctga cgctcgacac ccatacgctc
    11101 gccaccgaag cccaacaggt ggataacacc actaacattt tacgcctgat gttgctgctc
    11161 tcactggcaa tcggcgtagt gctgacccgc acgctgctac agggtaaacg tacccgctgg
    11221 cagcaatcgc ccttcctgtt aaccgccagc aaaccggtgc cggaagagga agaaagcgag
    11281 aaaaaagagt gacccattac tacaagaaag gaaatcgtta tgtccacatt acgcctgctc
    11341 atctctgact cttacgaccc gtggtttaac ctggcggtgg aagagtgtat ttttcgccaa
    11401 atgcccgcca cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt agtaattggt
    11461 cgcgcgcaga acccgtggaa agagtgtaat acccggcgga tggaagaaga taacgtccgc
    11521 ctggcgcggc gcagtagcgg tggcggcgcg gtgttccacg atctcggcaa tacctgtttt
    11581 acctttatgg ctggcaagcc ggagtacgat aaaaccatct ccacgtcgat tgtgctcaat
    11641 gctctgaacg cgctcggcgt cagcgccgaa gcgtccggac gtaacgatct ggtggtgaaa
    11701 accgccgaag gcgaccgcaa agtctcagga tcggcctatc gcgaaaccaa agatcgtggc
    11761 ttccaccacg gcaccttgct gctcaatgcc gacctcagcc gcctggcaaa ctatctcaat
    11821 ccggataaaa agaaactggc ggcgaaaggc attacctcag tgcgttcccg cgtgaccaac
    11881 ctcaccgagc tgttgccggg gatcacccat gagcaggttt gcgaggccat aaccaaggcc
    11941 tttttcgccc attatggcga gcgtgtagaa gcggaaatca tctccccaga caaaacgcca
    12001 gacttgccaa acttcgccga aacctttgcc cgtcagagta gctgggaatg gaacttcggt
    12061 caggctccgg cattctcgca tctgctggat gaacgcttta cctggggcgg cgtggaactg
    12121 catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tgtttaccga cagcctcaac
    12181 cccgcgccgc tggaagccct cgccggacga ctccaaggct gcctgtaccg cgcagatatg
    12241 ctgcaacagg agtgcgaagc gctgttggtt gacttcccgg aacaggaaaa agagctacgg
    12301 gagttatcgg catggatggc tggggcggta aggtaactcg ctgattaaaa tatccaagga
    12361 aggaaatatg atcgatctgg aaaaccagga acgggagatt atcaatttaa tgttcagcca
    12421 gcgtatttcc tggctagccg ccgtgcgtat caggcataaa ttatcgcttg ctgaagtaag
    12481 taaaatgtta ggtatctcga ttaattctct aaaacagatc gaaaagacgg aacggcttag
    12541 ctcaaacatc aaaagcaaaa tggcagaaat ttatggttgt ccaccagagt tgctgatatg
    12601 cccttcctgg atgacggctg aacataaatg aatagattcg tgaatagata ctaaaaagcc
    12661 ggagcagtcc cccactccgg cttaacacga caatcgcctt gcagcgaaac acaattactc
    12721 tttatcgccc agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg
    12781 acgctcagcg gcagtggtct gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt
    12841 cagggctttc gcgccaaatt ctgcagcgac gccgtagata ccagccgctt ccatttccac
    12901 gccgaggatg ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag
    12961 gtcagcggag aacaggttac ccacgcgagc atcaatgccc agtgctttag ctgcatctac
    13021 tgcgttacgc accatgtcga agtcagcgat agctgcgaag tcatggtctt taaagcggat
    13081 acggttaact ttggaatcgg tgcaggcacc cataccaatc accacgtcac gcagttttac
    13141 gtgcggcaga acagcgccac aggaaccaac gcggataatt ttcttcacgc cgaaatcggt
    13201 gatcagttct ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga
    13261 aattttgcgg cctttgtaag taccagtgaa gcccagcata ccgcgtacgt tgttcacttc
    13321 acgggcatct tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat
    13381 caaaactacg tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt
    13441 atcctttttg tgacataaca aaggcggagt caacgctccg cccaaatcca tcagaacatg
    13501 gctttgccat attccatatc agaagtacca aaatattttg ccagagtctg gccgatatcc
    13561 gcgaaggttt cacggtgacc cagtgagccc ggttttactt tcgggccgta aaccagtacc
    13621 ggaatgtgtt cacgcgtgtg gtcagtaccg gtccaggtcg gatcacagcc gtggtcagcg
    13681 gtaaggatca ggatgtcgtc atcgcgcagc agagacatca gctccggcag acggcggtcg
    13741 aacagttcca gacccgcagc ataaccggcg acgtcgcgac ggtggcccca ggaagagtcg
    13801 aagtcaacga agttggtgaa gacgatggtg ttatcacccg cttctttcat ctctttgatg
    13861 gtggcgtcaa acagcgcgtc caggccagtc gctttcactt ttttggtgat accgcagttg
    13921 gcgtagatgt ccgcaatttt accgacagaa accacctggc cgtgtttttc atcaaccagt
    13981 ttctgcagca cggtcggtgc tggcggctca acagccaggt cgtgacggtt accggtacgc
    14041 tggaagttac cggctttgtc gccgataaac ggacgagcga taacacgacc gatattgtag
    14101 ccgccgttgg tcagctcttc acgggcgatt tcgcacagtt cgtagagttt atccagaccg
    14161 aaagtttctt catgacaggc aatctggaac acggagtcag cggaggtata gaaaatcggc
    14221 ttgccggttt tcatgtgctc ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg
    14281 cagttaccga ggtaacccgg cagattagcg cgttcgacca gtttatccag cagctcttgc
    14341 gggaagctgt tttcgtgatc ggagaaatat ccccactcaa acagaaccgg gacaccggcg
    14401 atttcccagt gaccagacgg ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac
    14461 gcgccgataa cttcagcgtt gccgtccatt cccgccggaa tgaaaccggt agaaccttcg
    14521 tgtgctttcg ccagccccag acgggtcaga tttggcagat tgagcgggcc tttacgaccg
    14581 ttatcagctt cgcctttggc acaagcttca gcgatatgac ccagggtgtc agccccgacg
    14641 tcaccaaagc gttctgcatc ttctgtagcg ccgatgccga atgagtccag caccataata
    14701 aatgcacgtt tcatatgttc tccgtactca gtgcttcaaa tttaaatgat cagatcagta
    14761 taccgttatt cgctgatacg gcgatagaca gttggtgtgc tttccggtgc tttatcggca
    14821 agtttaattg ccgctttcac cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg
    14881 tggataaccg ccagcggacg ctgaccgtct acctggtcgc ccagacgtgc catatcagta
    14941 aagccgacgc tgtaatcgat ggtgtcagat gcctgacggc gtccgccgcc cattgcaacc
    15001 actgccatcc ccagcgcgcg ggtatccatt tcactgacaa aaccttcggt atcagcatag
    15061 actgctttcg tcagcatcgc tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc
    15121 gggccttttt gtgccgctac catacgacca aagacttctg ccgctttacc gttgtccagc
    15181 accgcctgca atttcgcgcg cgcttcggcg tcatctttcg ccagtttgcc ggagatcagc
    15241 atctccacac acagcgccat cgtgacatca aacagacgcg ggttacgata ttcacccgtc
    15301 aggaactgca ccgcttcacg aacttcaacc gcgttacctg cactggaggc cagtacctga
    15361 ttcatgtcgg tgagcagcgc ggtggtgcgc acgccagcgc cgttagccac gccaacaatc
    15421 gcttcggcaa gggcttcaga gagttcgtag gtcggcataa acgcgccgct acccactttc
    15481 acgtccatca ccagcgcgtc cagaccttcc gccagtttct tcgccaggat cgaggcagtg
    15541 atcagcggga tggagtccac ggttgcggta atatcgcggg tcgcgtagaa acgtttatct
    15601 gccggagcca gtgagctggt ctggccaata atcgccacac cgacgtcttt aataatttcg
    15661 cggaaacggt tgtcatccgg gaaaatgtcg aagccaggga tggattccag tttgtcgagc
    15721 gtaccgccag tatgaccgag gccacgaccg gagatcatcg gaatatagcc gccgcaggct
    15781 gcgaccatcg gccccaacat cagcgaagtg acatcgccga cgccaccggt ggagtgttta
    15841 tcaacaatcg gtccattcag atgcaggctt ttccagtcga gaacggttcc tgaatctcgc
    15901 atcgccatgg tcagcgagac acgctcaggc attgtcatat cgtggaagaa aatggtcatc
    15961 gcgagggcgg caatctgccc ttcggagata gtgttgtcgc gaataccgtt gataaagaaa
    16021 cgaatttctt catcgctcag cgcatgacca tcacgttttt tacgaataat ttcttgtgcg
    16081 agaaacaagg taacctcccg aggaaaagag tatgaggcgg cttttgccgg atgcgtctac
    16141 gccttatccg gcctacgggt agatacgtct cgtaggcctg ataagcgcag cgcatcaggc
    16201 gtaaagcatc ttacttagta gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc
    16261 agcaggcttg ccagcaggct ggaagcgcca aagcggtagt gacgcgcatc agcccagtca
    16321 gcaccgaaca gttcatctgc gatggcgaga tatttctgcg catcttccgc agtacgcacg
    16381 ccgcccgccg gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc
    16441 atgatgcgcg cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg
    16501 aagtccgcac ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc
    16561 agttcgccgg tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta
    16621 caggctttca ccaggtcaaa accaacctgc tcgttacccg ccatcagcgc gcggtacggg
    16681 aacacaacgt caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagtgcg
    16741 atgtcgatgt cgtcgttacc gtgtgggaag ttggttaccg tagcaatacg gatttccggg
    16801 gtgccctgct ctttcagtgt tttgcgagca atcgggataa agcgaggata gatacagata
    16861 gcggcggtat tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg
    16921 gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct
    16981 ttcagatcag tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg
    17041 ttagtattct aacatctact ccgcaacaca cttcgatata catcacaatt aaggaaatct
    17101 acttacaagt ttgcatcact ataatgcgat ctggttcaaa taattcactt tcaaatgaat
    17161 gcgtcagtgg tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg
    17221 cgcatcacct tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca
    17281 acaacgttgc cagcaaattg tcaccagccc agtgcttagc ccggagcaga agcgccattt
    17341 tctggcactg gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg
    17401 cgcgctggat gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg
    17461 ctatgtctta cccgattacg cccgttttct ggcgaacggt tccgaatggc tggagctgga
    17521 aggcgcgaaa gatcttgatg acgcactctc tctgctgacc attctttacc accacgtacc
    17581 gtcggtcaca tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt
    17641 tagaattcta acacaagacg agatcgatgt tcgaataaaa cgtttctggc gttacctcga
    17701 cagaaccctg ccagacgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac
    17761 ccgcgcgatc ttacgtgcag acgcagagtt gaagcaggtt tcaccgaacc tgacctttat
    17821 ctacgatcct gaaatcaccc ctgatgacct gctgctggaa gtggcgaaga acatctgtga
    17881 atgtagcaaa ccgcacatcg ccaacggtcc ggtgcatgat aaaattttca caaaaggggg
    17941 ctacgggatt gtgagctgtt acaactcact gccgctggcg ggtggtggca gcacgctggt
    18001 acgccttaac ctgaaagcca ttgccgagcg cagcgaatcg ctggatgact tctttacgcg
    18061 cactctaccg cactactgcc agcagcagat cgccatcatc gatgcgcggt gtgaattcct
    18121 ctatcaacaa tcacacttct ttgagaatag cttcctggtg aaagaagggc tgattaaccc
    18181 tgaacgtttt gtgccaatgt ttggcatgta tgggctggcg gaagcggtta acttgctgtg
    18241 tgagaaagaa gggattgccg cgcgctacgg taaagaagcc gccgcaaatg aagtgggtta
    18301 tcgcatcagc gcgcaactgg cggagtttgt cgccaatacc cccgtgaaat atggctggca
    18361 aaaacgcgcc atgttacacg cacagtcggg gatcagttcc gatatcggca ccacgccggg
    18421 cgcgcgtttg ccgtatggcg atgagccaga tccgatcacc catctgcaaa ctgtcgcccc
    18481 gcatcatgct tattattatt ccggcatcag cgacattctg acgctcgacg aaaccatcaa
    18541 acgtaatccg caggcgctgg tacagctttg cctcggtgcc tttaaagccg gaatgcgtga
    18601 atttaccgcc aatgtcagcg gtaacgatct ggttcgcgtt accggttata tggtgcgttt
    18661 gtcggattta gaaaaatatc gcgccgaagg ttcacgcacc aacaccacct ggctgggcga
    18721 agaagccgca cgcaacactc gtattctgga acgccagccg cgcgtgataa gccatgaaca
    18781 gcagatgcgc tttagtcagt aagattatcc ccttctcctg cgttgacggg ccaggcagtc
    18841 gtctggctct gtttttgcag ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga
    18901 cgatgggacg ttgcaatgac tgtggggagt gcgtgccaca gtgtccgcat caggcgttgc
    18961 agattgttga cggcaaagtg gtgtggaacg ctgtggtctg cgagcaatgt gatacctgcc
    19021 tgaagaggtg tccgcaacat gccacaccaa tggcgcaatc catgagcgtg gaggaggtgc
    19081 ttagccatgt gcgcaaagcg gtgctgttta ttgaagggat aacggtaagc ggcggcgaag
    19141 ccacgaccca actgccgttt gtggtggcgc tgtttactgc tatcaaaaac gatccgcaac
    19201 tgcgccacct cacctgtctg gtggacagta acggcatgtt gagcgaaacc ggctgggaaa
    19261 aattgctccc ggtgtgcgac ggcgcaatgc tcgatctcaa agcgtggggg agcgaatgtc
    19321 atcagcaatt aaccggacgc gataatcagc agattaagcg cagcatctgt ttgctggcag
    19381 agcgcggtaa gctggcggaa ctgcgtttgc tggtaatccc tggccaggtg gattatttgc
    19441 aacacatcga agaactggcg gcgtttatca agggagtcgg cgatgttccg gtacgcctga
    19501 acgcgtttca tgcccacggc gtgtatggcg aggcgcaaag ctgggcgagc gccacgccgg
    19561 aagacgttga gccgttggct gatgcgttac gggagcgcgg ggtgagccgg ttaatatttc
    19621 cggcgctgta tttgtgacat gccggatacg gcctacggtt cggcacagac ttgtaggcat
    19681 gataagacgc gtcaagcgtc gcatcaggca ttatgcgccg actgccggat gcggcgtgaa
    19741 cgccttatcc ggcctacggt tcggcacaaa cctgtaggca tgataagacg cgacaagcgc
    19801 cgactgccgg atgcggcgtg aacgccttat ccggcctacg gttcggcaca gacttgtagg
    19861 catgataaga cgcgacaagc gtcgcatcag gcattgtgcg ccaactgccg gatgcggcgt
    19921 gaacgcctta tccggcctac ggttcggcac aaacctgtag gcatgataag acgcgttaag
    19981 cgtcgcatca ggcattatgc gccgactgcc ggatgctgcc tacgaagggc aaactaccca
    20041 cgaacgttaa acaccgcata cgtgttatta agcaacactt cggcaatctc atccgcgggt
    20101 tccgggcgta actcgcaaag cacggcaaac acacgggcag catgctccgg gcggttaggc
    20161 tgcccctgaa aaccgttgag cggcatatcc ggcgcatcgg tttccagcaa caacgatgcc
    20221 agcggtaatt ttgcgatgac atcgcgggtt ttactggcgc gtggataggt gatagtaccg
    20281 cctacgccga ttttgtagcc cagcagtaca aaccgttcag cctgttgctg gctgccagaa
    20341 aatccgtgga caacaccagc acgcggcaaa tcgtggcgtt taagatgcat cgccagtttg
    20401 tcgtgcgtgc gccgtgaatg caggatcacc ggcagatcgt agcgtttcgc cagtttcagt
    20461 tgttcgtcga gtaaccactg ctgcctctca aattgcggat cgtcgccgaa gagatccaga
    20521 ccgatctccc ccaccgccac caccttcgcc ggacgccttt ccagcgcctg ctgtagctga
    20581 tcaagagaca catcgctatg tttttccaac ataccaggat gcaagcccaa tgcggcatac
    20641 agcggttgat aattttccgc taacgcctgc acgcgggcaa aattttccgc ctctgtcgcc
    20701 ggaacaatga tcttgcctac gcccgcttgt gccgcgcgtt gcaggctggc ctcttcatcg
    20761 ccactaaacg gcgggaaatc aaaatggcag tgggtgtcga taaaacggca aatcaagcca
    20821 gatcctcatt gttaaatgtc gggtcgttcg cctgcggagc gtcgatcact tcggcaacca
    20881 gcgtatcgtt ggcgactggc gcaggcggaa tgacaatcga ttccggcgtc accactggca
    20941 ccagatggcg ggtaagcggc gctttttcag ttaatagctt gccaaccgtt gccaggaaat
    21001 aacgaccgca taaacgccca agtttatagt cttcgcgcag cgccggaatc cgactgccaa
    21061 gcgcgatact atgtaatggc ttcggcggat aaatttcgaa tatccgcagc ttgcccggtg
    21121 gtttctcaat aaattgctga atgtcacgat agctggtttc atgatgctgc accagattga
    21181 ccagcggctg caggctactg tcacccagcc agcgttccat gcgtttgaac cactgcggcg
    21241 tgtagtacat ttgtgacggc acagtgcgga tgacgaccaa cgttttagcg ccctgccttg
    21301 ccgcctcttt aaccggaatc gcatcactga tcccgccatc caggtagtta atgccttcca
    21361 gcgacactcc gctacgataa aagccaggta tcgcactgga ggcgcgaatc acatccagcc
    21421 agttttgttt ggttggtaaa aagtaattcg gcgcgtagtc atcctgacga caggcgcaca
    21481 tataaaacga tttgccgctg tcaaacaacc gcgcggcggt atccatttgc aacggcatct
    21541 ggcttgcggt ggcctccacc agccagtcga gatcgataag atttcctcca cggacaaagc
    21601 gcaatggatc gaaaaattcg cgttttgtgg tatagcgcat gatgactttg cgcgcgtaac
    21661 cgggctgatt gcagataaac gccgagaggt tctgcgcccc ggcagatgtg ccgagataaa
    21721 gatcgaaagg attaaactgc gcgcgcataa actcatccag cacgccagcc gtgaaaattc
    21781 cacgctgccc gccgccttcg cacaccagag caattcgtcc gggctggaac ggccttagcg
    21841 acaacggcgc aatattacca agcgttacag gtattcgctg ccccacctct gccttcctgt
    21901 ttttgttgtg aaagaatgac aaagtaacgc aatttattat tcgctaaaac cgcaaaagcc
    21961 agtccgctgg actggctttg gataaaattt tcttaatggt ctgaaatcta gggtcgtttt
    22021 cggcccatga acaaactcac caggaacaga ataatcccga cgacaaagac aatttttgct
    22081 gcgcctgcag cggtaccggc cagaccacca aatccaagtg cggcggcgat taacgcgata
    22141 accagaaata tgatgcccca acgaaacata agcgtctcct ttaccatagt taatgtcacc
    22201 gcttgatatg agcgtgaaaa tcactcaatg cgatattttt agtgtggtgc acatcgcgcc
    22261 tcccgacaaa gttcgggagg acgaattacg acgaattact tagttttcag atcattttta
    22321 acgcttttca caccatctac cgctttggcg atactttcag cacggtcact ttgtgcctga
    22381 gaatctacgg taccggagag ctgaaccacg ccatcggtgg tttcaacttt cacatgacgg
    22441 gaagggacga tatcgtccgc cagcagtttg gctttgattt cactggtggt ggcggtgtca
    22501 cccgcgtagc ccttcaccga gccttcttta gcgtcgcgaa cgtgcagttt gtcgctgaca
    22561 gaggtcaccc cttcaacgcc tttcgccact ttcactgcct cttcggcctg ggcctggctt
    22621 tcaacgaaac cgctcagggt cacgactttt tgatcggttt tgacagagat atcggtgctc
    22681 ttgatgttgt catgatccac cagggccgcc ttcactttcg cggtgatggc gctgtcatcc
    22741 atgaaattac cgactttatt catagagcta tcgacttttt gccctgcgct ttcattggta
    22801 gtctgtgcgt tgttttccgc gtaggcagag ccggtcgcga cggcagaggt caacattaca
    22861 gccagcagag ttttcgaaat cttcagtctt gtcatagtca tcgatttatt cctgtatgtt
    22921 tgctcgtaat ttgagcctgg caacacgagg ttgcattgct gaatagggag agacttcacc
    22981 ctctacagaa gtcaatggtc gccatcacaa aagcgatgag tgatgaataa cgaccattac
    23041 agcctctgaa tcagttatta atatcggtag aatgacaatc gacggcttta gatactgata
    23101 tctacgcatt gaacggtatt taacgccgtc agaaatgtca tcactttgtt aaatatagat
    23161 cacaattttg aaaccgctcg ggatatcagc gaaaacataa gcaaaagtga atgttttaag
    23221 aacattccgt aagcggctaa taaggaaggg aaattgacag ggcgcagcgg ctaccgcgcc
    23281 ctggaggcaa gaattaatgc tcgcgggtct ggtggaactg aacgtccgga taacgttcct
    23341 gtgccaggcg caggttgacc atgctggtag cgatgtaagc gaggttatcg ccgccatcaa
    23401 gcgccagttg gctttcgttc ttacgcttga actcttcgaa tttcttcgcg tctgcacatt
    23461 ctacccagcg ggcagtggca acgttgactg actcatacac tgcttcaacg ttgtattcgc
    23521 tcttcaggcg cgctaccacc acatcaaact gcagcacacc aactgcacca acgatcagat
    23581 cgttgttgga gattggacgg aacacctgca ccgcgccctc ttcggaaagc tgtaccagcc
    23641 ctttgagcag ctgtttttgc ttcagcggat ctttcaggcg gatacgacgg aacagttctg
    23701 gtgcgaagtt cggaataccg gtgaacttca tcatctcacc ctgggtaaag gtgtcgccga
    23761 tctgaatggt gccgtggttg tgcaggccga ggatatcgcc cggatacgct tcttcaacgt
    23821 gcgaacggtc acccgccata aaggtcagcg cgtcggagat caccacatct ttcgcagtgc
    23881 gcacctggcg cagtttcatg cctttttcat atttaccgga caccacacgc ataaacgcca
    23941 cgcggtcgcg gtgtttcggg tccatgttgg cctgaatttt aaatacgaag ccggtaaatt
    24001 tatcttcgct cgcttctacg gtacgggtat cggtctgacg cggcatcggc gcaggcgccc
    24061 actccaccag gccatccagc atatgatcaa cgccgaagtt acccagcgca gtaccgaaga
    24121 ataccggagt gatttcgccc gcaaggaaca gctctttgtc gaactcgtta gacgcgcctt
    24181 taaccagttc cagttcgtca cgcagctgct gtgccagatc ttcaccaacc gcagcatcga
    24241 gatccgggtt attcagccct ttaacaatgc ggacttcctg aatggtgtgc cctttaccgc
    24301 tctgatagag ataggtttca tctttataaa ggtggtaaac gcctttaaac agcttgccgc
    24361 agccaattgg ccaggtgatc ggtgcgcagc caattttcag ctcgttctca acttcatcga
    24421 gcaattccat cgggtcgcgg atatcacggt caagtttgtt cataaaggtg aggatcggcg
    24481 tgtcgcgcag acgggtaact tccatcagct tacgggtacg atcttcaaca ccttttgcgg
    24541 cgtcgataac catcaggcag cagtccaccg ccgtcagggt acgataggta tcttccgaga
    24601 agtcttcgtg ccccggggtg tcgagcaggt taaccaggca atcgtgatac ggaaactgca
    24661 tcacagacgt agtaatggag atcccacgct gcttttccat ctccatccag tccgacttag
    24721 cgtgctggtt ggaaccacgg ccttttactg taccggcagt ctgaatggcc tgtccgaaca
    24781 gcagcacctt ctcggtgatg gtagtcttac cggcgtccgg gtgagaaata attgcaaagg
    24841 ttcttctttt cgctacctct gcggccaaaa ggcttgtcat aattgttgtc tctttgatta
    24901 tgtacaatca tatgtacaaa gaaaaattca tgccgctgat tgtacatttt ggaggtatca
    24961 gagcatagca gaacatgatc agtaggtgta tttgtagcca aagaacattt gtacatgcag
    25021 caatctttgt tctattgaca ctgtttattc atacagttaa aaataacact gtatacaaac
    25081 acagtgtaga gggattttta tgcgtattga gatctgcata gccaaagaga aaatgactaa
    25141 aatgccaaac ggtgctgtgg atgcgttaaa ggaagaatta actcgacgca tcagtaagcg
    25201 ttatgacgat gtagaggtga tcgtaaaagc caccagcaac gatggccttt ctgttacgcg
    25261 caccgccgat aaagattcag ctaaaacttt tgttcaggaa actctgaaag atacctggga
    25321 gtctgctgac gagtggtttg ttcactaatt aacacgtaaa atcggtaacg gctggaaatc
    25381 attcaatact cgcactatcg gaagttcacc agccaaccgc agcacgttct tgcatacgac
    25441 gtgtctgcgg tttttcaacc attcagatac agcactctca tcacattcat tcttccgcat
    25501 taaagtatat atgaccatat caccttaata tttttcttct tcagataaaa attgttatct
    25561 atgtatactt ttaaaccgaa tccgtgtaga gtctctacct aagttagttc gcagctaccc
    25621 cttcagtttg cgccattaac ctcctgactt ttgccgctat ctgtaagcat tcctgtatac
    25681 ctgaaactac agggttcatc tacgaacttt aagaacaccc aggataaaaa ttgtcaacta
    25741 tattatatat aacaccatac taattcgagg ctatatgaac agcatactga taatcacttc
    25801 actacttatc atattcagca tttttggtca tgctctaata aaattaggga ttggcatatc
    25861 caataacccg gacaaaaccg atgtataagt caacatat
//
LOCUS       DAZKFU020000044        25804 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZKFU020000044 DAZKFU020000000
VERSION     DAZKFU020000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.044"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..117)
                     /gene="shoB"
                     /locus_tag="ACHOQQ_RS07210"
                     /old_locus_tag="pgaptmp_001442"
     CDS             complement(37..117)
                     /gene="shoB"
                     /locus_tag="ACHOQQ_RS07210"
                     /old_locus_tag="pgaptmp_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGU4469712.1"
                     /translation="MTDCRYLIKRAIKIIIAVLQLILLFL"
     gene            complement(538..918)
                     /gene="acpS"
                     /locus_tag="ACHOQQ_RS07215"
                     /old_locus_tag="pgaptmp_001443"
     CDS             complement(538..918)
                     /gene="acpS"
                     /locus_tag="ACHOQQ_RS07215"
                     /old_locus_tag="pgaptmp_001443"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU4469713.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(918..1649)
                     /gene="pdxJ"
                     /locus_tag="ACHOQQ_RS07220"
                     /old_locus_tag="pgaptmp_001444"
     CDS             complement(918..1649)
                     /gene="pdxJ"
                     /locus_tag="ACHOQQ_RS07220"
                     /old_locus_tag="pgaptmp_001444"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGU4469714.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRNACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1661..2389)
                     /gene="recO"
                     /locus_tag="ACHOQQ_RS07225"
                     /old_locus_tag="pgaptmp_001445"
     CDS             complement(1661..2389)
                     /gene="recO"
                     /locus_tag="ACHOQQ_RS07225"
                     /old_locus_tag="pgaptmp_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGU4469715.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2401..3306)
                     /gene="era"
                     /locus_tag="ACHOQQ_RS07230"
                     /old_locus_tag="pgaptmp_001446"
     CDS             complement(2401..3306)
                     /gene="era"
                     /locus_tag="ACHOQQ_RS07230"
                     /old_locus_tag="pgaptmp_001446"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGU4469716.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3303..3983)
                     /gene="rnc"
                     /locus_tag="ACHOQQ_RS07235"
                     /old_locus_tag="pgaptmp_001447"
     CDS             complement(3303..3983)
                     /gene="rnc"
                     /locus_tag="ACHOQQ_RS07235"
                     /old_locus_tag="pgaptmp_001447"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGU4469717.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4256..5230)
                     /gene="lepB"
                     /locus_tag="ACHOQQ_RS07240"
                     /old_locus_tag="pgaptmp_001448"
     CDS             complement(4256..5230)
                     /gene="lepB"
                     /locus_tag="ACHOQQ_RS07240"
                     /old_locus_tag="pgaptmp_001448"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGU4469718.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5246..7045)
                     /gene="lepA"
                     /locus_tag="ACHOQQ_RS07245"
                     /old_locus_tag="pgaptmp_001449"
     CDS             complement(5246..7045)
                     /gene="lepA"
                     /locus_tag="ACHOQQ_RS07245"
                     /old_locus_tag="pgaptmp_001449"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGU4469719.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7243..7722)
                     /gene="rseC"
                     /locus_tag="ACHOQQ_RS07250"
                     /old_locus_tag="pgaptmp_001450"
     CDS             complement(7243..7722)
                     /gene="rseC"
                     /locus_tag="ACHOQQ_RS07250"
                     /old_locus_tag="pgaptmp_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGU4469720.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7719..8675)
                     /gene="rseB"
                     /locus_tag="ACHOQQ_RS07255"
                     /old_locus_tag="pgaptmp_001451"
     CDS             complement(7719..8675)
                     /gene="rseB"
                     /locus_tag="ACHOQQ_RS07255"
                     /old_locus_tag="pgaptmp_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGU4469721.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8675..9325)
                     /gene="rseA"
                     /locus_tag="ACHOQQ_RS07260"
                     /old_locus_tag="pgaptmp_001452"
     CDS             complement(8675..9325)
                     /gene="rseA"
                     /locus_tag="ACHOQQ_RS07260"
                     /old_locus_tag="pgaptmp_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGU4469722.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9358..9933)
                     /gene="rpoE"
                     /locus_tag="ACHOQQ_RS07265"
                     /old_locus_tag="pgaptmp_001453"
     CDS             complement(9358..9933)
                     /gene="rpoE"
                     /locus_tag="ACHOQQ_RS07265"
                     /old_locus_tag="pgaptmp_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGU4469723.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9930..10085)
                     /gene="rseD"
                     /locus_tag="ACHOQQ_RS07270"
                     /old_locus_tag="pgaptmp_001454"
     CDS             complement(9930..10085)
                     /gene="rseD"
                     /locus_tag="ACHOQQ_RS07270"
                     /old_locus_tag="pgaptmp_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGU4469724.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10341..11963
                     /gene="nadB"
                     /locus_tag="ACHOQQ_RS07275"
                     /old_locus_tag="pgaptmp_001455"
     CDS             10341..11963
                     /gene="nadB"
                     /locus_tag="ACHOQQ_RS07275"
                     /old_locus_tag="pgaptmp_001455"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGU4469725.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERN
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVHGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11948..12685)
                     /gene="trmN"
                     /locus_tag="ACHOQQ_RS07280"
                     /old_locus_tag="pgaptmp_001456"
     CDS             complement(11948..12685)
                     /gene="trmN"
                     /locus_tag="ACHOQQ_RS07280"
                     /old_locus_tag="pgaptmp_001456"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGU4469726.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12817..14151
                     /gene="srmB"
                     /locus_tag="ACHOQQ_RS07285"
                     /old_locus_tag="pgaptmp_001457"
     CDS             12817..14151
                     /gene="srmB"
                     /locus_tag="ACHOQQ_RS07285"
                     /old_locus_tag="pgaptmp_001457"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGU4469727.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14184..15065)
                     /gene="yfiE"
                     /locus_tag="ACHOQQ_RS07290"
                     /old_locus_tag="pgaptmp_001458"
     CDS             complement(14184..15065)
                     /gene="yfiE"
                     /locus_tag="ACHOQQ_RS07290"
                     /old_locus_tag="pgaptmp_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4469728.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            15168..15755
                     /gene="eamB"
                     /locus_tag="ACHOQQ_RS07295"
                     /old_locus_tag="pgaptmp_001459"
     CDS             15168..15755
                     /gene="eamB"
                     /locus_tag="ACHOQQ_RS07295"
                     /old_locus_tag="pgaptmp_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGU4469729.1"
                     /translation="MTPTLLSAFWTYTLITALTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISYSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15810..16193)
                     /gene="grcA"
                     /locus_tag="ACHOQQ_RS07300"
                     /old_locus_tag="pgaptmp_001460"
     CDS             complement(15810..16193)
                     /gene="grcA"
                     /locus_tag="ACHOQQ_RS07300"
                     /old_locus_tag="pgaptmp_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGU4469730.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16498..17187
                     /gene="ung"
                     /locus_tag="ACHOQQ_RS07305"
                     /old_locus_tag="pgaptmp_001461"
     CDS             16498..17187
                     /gene="ung"
                     /locus_tag="ACHOQQ_RS07305"
                     /old_locus_tag="pgaptmp_001461"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGU4469731.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(17235..18272)
                     /gene="yfiF"
                     /locus_tag="ACHOQQ_RS07310"
                     /old_locus_tag="pgaptmp_001462"
     CDS             complement(17235..18272)
                     /gene="yfiF"
                     /locus_tag="ACHOQQ_RS07310"
                     /old_locus_tag="pgaptmp_001462"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4469732.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18479..18898
                     /gene="trxC"
                     /locus_tag="ACHOQQ_RS07315"
                     /old_locus_tag="pgaptmp_001463"
     CDS             18479..18898
                     /gene="trxC"
                     /locus_tag="ACHOQQ_RS07315"
                     /old_locus_tag="pgaptmp_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGU4469733.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            18967..19665
                     /gene="tapT"
                     /locus_tag="ACHOQQ_RS07320"
                     /old_locus_tag="pgaptmp_001464"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             18967..19665
                     /gene="tapT"
                     /locus_tag="ACHOQQ_RS07320"
                     /old_locus_tag="pgaptmp_001464"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGU4469734.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAEAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19697..22357
                     /gene="pat"
                     /locus_tag="ACHOQQ_RS07325"
                     /old_locus_tag="pgaptmp_001465"
     CDS             19697..22357
                     /gene="pat"
                     /locus_tag="ACHOQQ_RS07325"
                     /old_locus_tag="pgaptmp_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGU4469735.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22471..23826
                     /gene="pssA"
                     /locus_tag="ACHOQQ_RS07330"
                     /old_locus_tag="pgaptmp_001466"
     CDS             22471..23826
                     /gene="pssA"
                     /locus_tag="ACHOQQ_RS07330"
                     /old_locus_tag="pgaptmp_001466"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGU4469736.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23872..24195
                     /gene="yfiM"
                     /locus_tag="ACHOQQ_RS07335"
                     /old_locus_tag="pgaptmp_001467"
     CDS             23872..24195
                     /gene="yfiM"
                     /locus_tag="ACHOQQ_RS07335"
                     /old_locus_tag="pgaptmp_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGU4469737.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24192..25490)
                     /gene="kgtP"
                     /locus_tag="ACHOQQ_RS07340"
                     /old_locus_tag="pgaptmp_001468"
     CDS             complement(24192..25490)
                     /gene="kgtP"
                     /locus_tag="ACHOQQ_RS07340"
                     /old_locus_tag="pgaptmp_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU4469738.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25607..>25804)
                     /locus_tag="ACHOQQ_RS07345"
                     /old_locus_tag="pgaptmp_001469"
     CDS             complement(25607..>25804)
                     /locus_tag="ACHOQQ_RS07345"
                     /old_locus_tag="pgaptmp_001469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469739.1"
                     /translation="KRKAILTDGLFALVKKMPDATLRVLYSHICQIQQRIRLPQLAHL
                     HTCSPHQKFILKLLNNHFPAN"
BASE COUNT         6231 a         6824 c         6440 g         6309 t
ORIGIN      
        1 ggttcaccgg acgcacctta agtacgtttc cttgtgttat aagaacagaa ggatcagctg
       61 taaaacagca atgatgattt tgatggcccg tttaataagg tatcggcaat cagtcattcg
      121 tttttcctta aacaaggaat gcagccgttc agattagcca ttacatctcc ccaaaactga
      181 acgtgcgagt tattgagggt gcatgctgca ctccacacca gagctttgac gacaccactc
      241 gtttcaatgg gggaattctg tggcatggtg taaagcacag caaaatcgcc aataacgaaa
      301 ccaattttaa tgtacttacg aattggcggt cacgttaatc ttgccatcaa tatcgctttc
      361 ttcgtaaagg ctcgagtttt tatgataaag attgcaagtt gcttgtaaaa gataactacc
      421 ctgatccata atcgctgttg ttgagggtgc gtgctgcact aaattaaagt tacaaagtaa
      481 aacccccgtt ccttaccagt tcgggggttt tactttttaa agagaacggt attattttta
      541 actttcaata attaccgtgg cgcaagcata gtggcgctca tcggccagcg tcacatgcat
      601 atttgcaacg cccagctttt ccgccagttt taatgcctcg ccccacagtc gcagccgtgg
      661 cttaccaagc tcatcgttaa atacttcaaa ctgattaaac gccagaccat tgcggatccc
      721 ggtgccaaac gcttttgctg cggcttcttt tacggcaaag cgtttcgcca gaaaacgcac
      781 cggctggtgg tgcgttttcc aaatttccca ttcgttatcg cttaatacgc ggcgtgccag
      841 gcgctcaccg gatcgggcga tcactgcttc tatgcgagcg atctcgacga tatccgtgcc
      901 taaacctaat attgccatta gccacgcgct tccagcatca ggcgcttcat ttctgccacc
      961 gcatctttca gtccggtcat cactgcacga ccaataatgg catgaccgat attcagttcg
     1021 tgcatctcag ggatggcggc aatggctttt acgttgtgat aggtcagacc gtgtccggcg
     1081 ttaactttca aaccgagact agcggcaaag gtcgcggctt tggcgatacg cgccaactct
     1141 tgcgcctgtt cggcgtcagt tttggcatca gcatagcaac cggtgtggat ctcgataaac
     1201 ggtgcgccaa cctctgccgc agctttgatc tgctcttcat cggcgtcgat aaacagagaa
     1261 acctgaatcc cggcatccgc cagacgtttg caggcattgc gcattttttc acgctgccct
     1321 gcaacgtcca ggccgccttc ggttgttact tcctgacgct tttctggtac caggcagcaa
     1381 aaatgcggct tcgtctcaac cgcgatcgcc agcatctctt cggtcaccgc catctccaga
     1441 ttcatgcggg tatccagcgt ctgacgcagg atgcgcacat cgcggtcagt aatgtggcgg
     1501 cgatcttcac gtaaatgcac ggtaatgccg tccgcccccg cctgctcggc aataaacgcg
     1561 gcctgcaccg gatccgggta agcggtaccg cgcgcgttgc gcagcgtagc gatatggtca
     1621 atgttgacgc ctaacagtaa ttcagccatg acaatcctca tcattcataa tgtgttttca
     1681 ccgttcgctt aggcataaat tgccggaaca gttccctgct ctttaaaggt ttaccgccaa
     1741 gatacggctt aagcgccatg cgggtaaagc gtttcgcggc gcgcagtgtg tctgcgtcag
     1801 gaaattcccg tgcgtttaac gcttttaact gccttccggt gaacgtttta ttgtcgataa
     1861 cgacgcttgc gataaaccct ttttcttcgc gataacgata cgtcatggtg tcatctaccg
     1921 gctcgccgct acccgcacaa tgggtaaaat tgacgccata acccagatgc ccgagcagtg
     1981 ccagttcaaa gcggcgcagc gcgggttctg gcgtaccagt gacccctgca agagactgaa
     2041 tgcagtgcaa gtaatcgaaa aagagttcag agaagcgcgt ctcgtattcc agtacgcggg
     2101 agagaagttc gttgatgtac agaccgctgt aaagcgtgat accgcttaat ggcagcgcca
     2161 gcgagacagc ttcagcactg cgcagcgttt tgacttcgcc acgcccgcca aaacgtagca
     2221 agagaggggt gaaaggctgt aatgcacctt tcagggttga gcgtttagag cgtgcgcctt
     2281 tggcaaccag acgcacgcga cccgattcct ccgtgaagac gtccagcatc aggctggttt
     2341 cgctccacgg gcgactatgc aggacaaatg cgcgctgcca gccttccatc ggagttactc
     2401 ttaaagatcg tcaacgtaac cgagactgcg cagtgcgcgt tcgtcgtcgg cccaaccgga
     2461 tttcactttt acccacagct caaggtgaac aggcgcttca aacatttcct gcatgtcttt
     2521 acgcgcttca atcccgatgg ttttgatctt ggcccctttg ttgccaatga ccatcttctt
     2581 ctgcccttca cgctcaacga gaatcaaacc gttgatgtca taaccaccgc gttcgttaga
     2641 gacgaaacgt tcgatctcca cggtcacgga gtacggcagt tcagcgccga ggaaacgcat
     2701 cagtttttcg cggatgattt cagacgccat aaaacgctgt gagcgatcgg tgatgtaatc
     2761 ttccgggaag tgatgagtcg cttcaggtag atgcttacgc acgattgccg caatagtgtc
     2821 aacattcagc ccggtttcgg cagagattgg cacgatatcg aggaagttca tctggcttgc
     2881 cagaaactgc aggtgcggca gcagatcggc tttctcctgc acgttgtcca ctttgttcac
     2941 cgcgaggatt accggcgctt tgccttcgcg cagtttgttg agcaccattt cgtcgtccgg
     3001 cgtccagcgg gtgccttcaa cgacaaaaat caccagctca acatcgccaa tagagctgct
     3061 cgccgctttg ttcatcaggc ggttaatggc gcgtttttct tccatatgca ggcccggtgt
     3121 atcgacgtag atcgcctgat acgcgccttc agtatggatc cccacaatgc ggtgacgagt
     3181 tgtctgcgcc ttgcgggagg tgatggagat tttctgcccc agcagtttgt tcaacaatgt
     3241 ggatttgcca acgttcggac gtccgacgat ggcaataaat ccgcagtaac ttttatcgat
     3301 gctcattcca gctccagttt tttcaacgcc tgttcggcgg cagcctgctc agccttacga
     3361 cggcttgaac ctgtgccaac caccggttca ctcaggccgc tgacctggca gtggattgta
     3421 aattcctgat cgtgcgcttc gccacgtacc tggactacca gataagtcgg cagcggcaga
     3481 tgacgaccct gcaaatattc ttgcaagcgc gttttcggat ctttttgttt atcgcctggg
     3541 ctaatttcgt ccagacgagt ttgataccag ttgaggatta atttctcgac ggtttgaata
     3601 tcactgtcga ggaatacgcc accaattaat gcttcgacgg tgtcggcgag aattgactca
     3661 cgacgaaatc caccgctttt aagttcacct ggtcctaaac gtaagcactc gcctaactca
     3721 aattcgcgcg ccagttccgc cagcgtattg ccacggacca gcgtggcgcg catccggctc
     3781 atatcgcctt catccacacg agggaaacgg tgataaagcg cattggcgat aacgtagctc
     3841 agaatagagt cgcctaaaaa ttctaaacgc tcgttatgtt tgctgctggc actacgatga
     3901 gttaatgcct gctgcaacag ttcctgatga ttaaaagtgt agcccagctt ccgttgaagc
     3961 cgattaatta cgatggggtt catgcgatac caataaataa atgcgtcaac aattcagcac
     4021 acgaaacaga cctgatatac atggctctgc taactgcttc gctgcagttt cagtttagcg
     4081 gtatatgggg accaacgctg tttcgtgtgc cgtggcaacc tggaggtgcc aaccttaaac
     4141 ttcgggggaa tattctatac acaacgacgg gggatgtcgt tagccacggg agatttatct
     4201 cataaataat tcacgttgtc gccataacgg cgacaacgtg aacgaagatg agctattaat
     4261 ggatgccgcc aatgcgactt aagcgcacac ctgtcggcca ttcgccttct tgcttatcga
     4321 agctcatcca gatagccgtt gcccgaccga ccagattcgc ttcaggcaca aagccccagt
     4381 aacggctgtc cgcgctgttg tcgcggttgt cgcccatcat gaagtattgt cccggaggaa
     4441 caatccaggt tgccagttgt tgccctggct gctggtaata catccccacc tgatcctgcg
     4501 caatcggcac tgtcagaatg cggtgcgtca catcacccag tgtctcttta cgctcggaaa
     4561 gacgaattcc attttctttg gtttcgtttt tcggcacttc aaagaatccg ctggtcgctt
     4621 ccccaccatt acggcgtgag aaggtctgaa cgaaatcgct cggttccacg tttgagtagg
     4681 tgaccggcag cgcgttttca cacgcctggc cggaactgca tcccggttga atcgtcagct
     4741 cttttgagac cggatcgtaa gtgactttat cgcccggtaa acccaccgcg cgcttgatgt
     4801 aatcaagctt tggatcttcc ggatatttaa agaccacgat atcgccgcgt ttcggatgac
     4861 cggtttcgat cagcgttttc tggtagatag gatctttaat gccataagca aacttctcta
     4921 ccagaataaa atcaccgatt aacagagtcg gcatcatcga acctgacgga atctggaacg
     4981 gttcataaat aaacgaacgc acaatcaata cgatagccag taccggaaaa acagaagcac
     5041 cggtttccag ccagccaggc ttcggcgcaa cctttttcaa cgttgcttta tccagtgagt
     5101 caccggcagc cgcctgcgcc gctgcctgac gttcccgccg tttaggtgcg aaaaagaatt
     5161 tatccacgca ccataaaatg cccgtcacca gtgtggcaat caccagaatc agggcaaaca
     5221 tattcgccat gccaactcct aagggttatt tgttgtcttt gccgacgtgc agaatggcga
     5281 ggaacgcttc ctgcggcagc tcgacgttac cgatctgctt catgcgtttc ttaccttctt
     5341 tctgcttctg cagcagcttt ttcttacggc tgatatcgcc gccataacat ttagccagta
     5401 cgtttttacg cagctgtttc acggtggatc gcgcaataat gtgcgtacca atcgctgcct
     5461 gaatggcgat atcaaactgc tggcgtggga tcagatcttt catcttctcc accaactcgc
     5521 gaccgcggtt ttgcgaatta tcacggtggg tgatcaacgc cagcgcatca acacgttcac
     5581 cgttgattaa tacgtctaca cgtaccatgt cggacgcctg gaagcgtttg aagttgtaat
     5641 ccaaagacgc atagccacgc gaggttgatt tcagtcggtc gaagaaatca agcaccactt
     5701 ccgccatcgg gatctcgtac gtcagcgcca cctgattacc gtggtaaacc atattggtct
     5761 gcacgccgcg tttttctacg cacagcgtaa taacgttgcc gagatatgcc tgcggcagca
     5821 gcatgtgaca ctctgcaatc ggctcgcgca gttcgtaaat gttatttacc gcaggcagct
     5881 tggatgggct gtcgacgtag ataacttccc ttgacgtggt ttcaacttca tacactacgg
     5941 tcggcgcagt ggtgatcaga tccagatcgt attcacgttc cagacgctcc tggatgatct
     6001 ccatgtgcag caggccaagg aagccgcagc ggaaaccaaa gcccagcgcg ctggagcttt
     6061 ccggctcata gaacagtgag gcatcgttca ggctgagttt acccagcgcg tcacggaagg
     6121 cttcatagtc gtcggaactt accgggaaca gaccggcgta tacctgcggt ttgactttct
     6181 taaagccagg cagcgccttt tctgccggat tacgcgccag cgttaaggta tcgccgactg
     6241 gagcgccgtg gatatcttta atcgcacata cgagccagcc tacttcgcca catttcagtt
     6301 cagtgcggtc aacctgtttc ggcgtgaaga tgcccagacg gtcggcgtta taggtctgcc
     6361 cggtactcat gactttcact ttgtcgccct tacgcagggt gccgttttta atacggataa
     6421 gtgaaacaac gcccaggtag ttgtcgaacc atgagtcgat aattagtgcc tgcaacgggc
     6481 cttccggatc gccttccggc ggcggaatgt cgcgcaccag acgttcgaga acgtcctgca
     6541 cgccaacgcc ggttttcgct gaacagcgca ccgcgtcagt ggcgtcgatg ccgacgatat
     6601 cttcaatttc ttccgccacg cgttcaggat cggctgccgg caggtcaatc ttgttcagta
     6661 ccggcactac ttcgagatcc atctccatgg cggtgtagca gttcgccaga gtttgcgctt
     6721 ccacgccctg cccggcgtcg accaccagca atgcgccttc gcaggccgcc agcgaacggg
     6781 aaacttcata ggagaagtca acgtggcctg gagtgtcgat aaagttaagc tgataggttt
     6841 cgccgtcaga cgctttgtag tccagcgtca cgctttgcgc tttgatggta atgccacgct
     6901 cacgctcaag atccatggaa tcgagaacct gcgcctccat ttcacggtca gacaggccac
     6961 cgcagatctg gataatacgg tcagacagcg tcgatttacc gtggtcaatg tgagctatga
     7021 tcgaaaagtt acgtatattc ttcataaagt atgattattg tgccttaatg cccggttaac
     7081 caggctttta gaagtcgctg ttctgagctt aacgtctgta ttaatagaaa cgccgcattc
     7141 tacactacaa cattgaggcg aggaaatgtt cataccgtat ggattgtggt atctggaaac
     7201 gtcctcgcat ttgttatgca aaatgcaaca aagccagtga aatcactggc tcgcgtcttc
     7261 cgaagatgtt tcaaatcgca caaggccagg cggcaatgcc acgcttaaga tgatcggttg
     7321 ccattctgcc cgggcagcaa acttacgcga gtagccacgc gcaatcagga acccgccaat
     7381 accgccgagg accgcaccac ataatgctgc gacgtcagaa gcaaagagta gctgaaatag
     7441 cgaagcgatg aggaataatc ccaccagcgg cgacatataa accagtaatg cggagctaag
     7501 caggctgcct tcggcgatcc ccaattccac tttttgcccc ggcactaacg gttcatcaca
     7561 gggtacgaca atggtatgcg tggtttgcgg gccaagttta ttcaacacgc ggctaccgca
     7621 accggcgcgt gaagcgcagc tgctgcatga ggctttaaca tcacaactga ccagcgcctg
     7681 cccgttttgc caggagacga cggtagccca ctctttgatc attgcgctgc cccgaactta
     7741 atattctcgg caatgcgttt cgccgtttgc ggcggcagtt caccgacaat ggtgatttcg
     7801 gcgttatcac gtacgcttgt actgacggtt ctgcgtccgg tgcgcaacat ctgatcggtg
     7861 ctcgatggcg tagcgcggtt aacgtttacc gagaagctga ataatccgtc ggaatagaga
     7921 cgtgattcga taggcatgtt gtccatcgtc ggtagcggac gtcgactact ggaaacttcg
     7981 ctaaaaccct gtggcaacca ggttggcgtc cagctgaatt tagctttttc acctacagga
     8041 acagaaagca acggcggcaa atttgccttc gccagcgtct gcatactgct gctgatatcc
     8101 tgattgacgt taaaagcaat cacgcgaaat tgttccagcg tttcaccatc gcgatcaaga
     8161 agatcaaccc gcatcggtaa tttcgattcg gtgtccatcc acacgatgta gctgtagcgt
     8221 gtgccatcac gagcaaccac gcgaataact tcacaaagac gatcagcaat acgcgtgcgc
     8281 ccgacggaga taaagtcgta gtaaggagaa aggcgtttga aatcggtata gatgagcgat
     8341 ggcagagaat caacaatgta atcgccatta agagtaaacg gttcaaggcc cggttcaaaa
     8401 tagctgattt cattgccgcg ctgtaccact tcccggcgcg ggccatccat ttgcaacaat
     8461 tgtgcaagag gacggttatc gaggcgtgca tgtcgataac gcagagactc aacaccctgt
     8521 ttattgatgc tgatgaatga cagctcgtaa ttcagtgact gactggccag gttcatctgc
     8581 tgtaataacg ccccggacgc gggagtggcc gaggcgttag cagagaataa caggctacct
     8641 gtcactaatg acatggcaaa ccaaagttgc ttcattactg cgattgcgtt cctaaagttt
     8701 gaattcctgg cacctgtaca gcggcttgct gggtttgcgc ctgctcaaac tgaagctgtt
     8761 cagagtggag tcggcgttgc agttcgtaat cctgcaacat tgcattaatg cgacgacgct
     8821 gctcctgtac ctgctgctgt tgaccattat ttgcggtcgc ttcagaaggt actcccaggc
     8881 taaccgggct ggctttaccc atcatcggca gtgtattaaa taccggtgtt tcgggctgct
     8941 gggacgtttc agattgtcca ttatagtgct ggacgccaac gataactgca agcgatacgc
     9001 atgcggctac gcccatttgg gtaagctgtg ccgcccacgg acgtactttc tgccagaatg
     9061 gcattttctg ccattgatgc ggcgcaggct gggcttccgg gatcaatgtc gccggttgac
     9121 gtactggctc ttcttcaatg gcggccatca cgcgtgaaga gatatcgaaa tggagcacct
     9181 cgggagtatc accccgcatt gagtcacgga ttaagtgata gctttcccag gttttctgca
     9241 tttctgggtt atgagccagt tcgttaagca gctcactatc cagcgtttcg ccatccatta
     9301 aagcggaaag ttgttctttc tgcatgccta atacccttat ccagtatccc gctatcgtca
     9361 acgcctgata agcggttgaa ctttgttatc aatagcttcc ctcgctcgga agatacgtga
     9421 acgcaccgta cctaccggac aatccatgat agcggctatc tcttcatagc tcaggccatc
     9481 cagctcccgc aaggttattg ccatgcgtaa atcttccggg agggactcaa tagttcggaa
     9541 aactatctgt ctcagttctt ctgacaacat taagttctca gggttcgaaa tttctttcaa
     9601 cgcgccgcca ctttcgaagt tttcagcttc aatggcatcc acatcactgg aaggtggacg
     9661 acgcccctga gcaaccaggt aatttttcgc tgtatttaca gcaatccgat acagccatgt
     9721 ataaaaagcg ctatctcccc ggaacgaatc cagcgcacga taggctttaa taaaagcttc
     9781 ttgtaccaca tcgggaacat cacccgacgg cacatagcgg gaaaccagac tcgccacttt
     9841 atgctgatag cgcactacca gtaagttaaa ggctttctga tctcccttct ggacccgttc
     9901 aaccaggacc tggtccgtta actgctcgct catccgaggt aaagtctccc caaaccaaat
     9961 ttccacgcgc tatcgaaacg ccactccatt agctgcaatt tgagcaagca aagggttaga
    10021 gtgtctcgtt tttgtaaagt tccgtaacgc atctgttttt gtttgtcatg ctgtagacgg
    10081 atcattatct atcattataa gtctacagaa tctgaacatc gcattatctg tgtagaaatg
    10141 cccatttaac tgcctgaaga gtaacccaac ggccttttta tttcaccacc taatcctcca
    10201 ccagccagta acttctcttt ttctcgccgc cctgcgtcag cgtgtttagc cactgtaaca
    10261 aatattaaaa tagcaggtgt ttattcgcac aacatgatgc tatgctgacc aaaccgtgtt
    10321 tagtaaatta aacaaagaaa atgaatactc tccctgaaca ttcatgtgac gtgttgatta
    10381 tcggtagcgg cgcagccggg ctttcactgg cgctacgcct ggctgaccag catcaggtca
    10441 tcgttctaag taaaggcccg gtaacggaag gttcaacatt ttatgcccag ggcggtattg
    10501 ccgccgtgtt tgatgaaact gacagcattg actcgcatgt ggaagacaca ttgattgccg
    10561 gggctggtat ttgcgatcgc catgcagttg aatttgtcgc cagcaatgca cgatcctgtg
    10621 tgcaatggct aatcgaccag ggggtgttat ttgataccca cattcagccg aatggcgaag
    10681 aaagctacca tctgacccgt gaaggtggac atagtcaccg tcgtattctt catgccgccg
    10741 acgcaactgg tagagaagta gaaaccacgc tggtgagcaa ggcgctgaac catccgaata
    10801 ttcgcgtgct ggagcgcaac aacgcggttg atctgattat ttctgacaaa attggcctgc
    10861 cgggcacgcg acgggttgtt ggcgcgtggg tatggaaccg aaataaagaa acagtggaaa
    10921 cctgccacgc aaaagcggtg gtactggcaa ccggcggtgc gtcgaaggtt tatcagtaca
    10981 ccaccaatcc ggatatttct tctggcgatg gcattgctat ggcgtggcgc gcaggctgcc
    11041 gggttgcaaa tctcgaattt aatcagttcc accctaccgc gctgtatcac ccacaggcac
    11101 gcaatttcct gttaacggaa gcactgcgcg gcgaaggcgc ttatctcaag cgcccggatg
    11161 gcacgcgttt tatgcccgat tttgatgtgc acggcgaact ggccccgcgc gatattgtcg
    11221 cccgcgccat tgaccatgaa atgaaacgcc tcggcgcgga ttgtatgttc cttgatatca
    11281 gccataagcc cgccgatttt attcgccagc atttcccgat gatttatgaa aaattgctcg
    11341 ggctggggat tgatctcaca caagaaccgg taccgattgt gcctgctgca cattatacct
    11401 gcggtggtgt aatggttgat gatcatgggc gtacggacgt cgagggtttg tatgccattg
    11461 gcgaggtgag ttataccggc ttacacggcg ctaaccgcat ggcctcgaat tcattgctgg
    11521 agtgtctggt ctatggctgg tcggcggcag aagacatcac cagacgtatg ccttatgccc
    11581 acggcgtcag tacgttacca ccgtgggatg aaagccgcgt tgagaaccct gacgaacggg
    11641 tagtaattca gcataactgg cacgagctac gtctgtttat gtgggattac gttggcattg
    11701 tgcgcacaac gaagcgcctg gaacgtgccc tgcggcggat aaccatgctc caacaagaaa
    11761 tagacgaata ttacgcccat ttccgcgtct caaataattt gctggagctg cgtaatctgg
    11821 tacaggttgc cgagttgatt gttcgctgtg cgatgatgcg caaggagagt cgtggcctgc
    11881 attttacgct ggattacccg gaactgctca cgcattccgg tccatcgatc ctttctcccg
    11941 gcaatcatta cataaacaga taaaaagcct gggttagcgc cgtatacgct tcggaatagt
    12001 tctggtctgg cccacgaatg actaagcgat cgctaaagca ttctcccgcc tgcggggaga
    12061 atgccagcag cacccgatgc ggcagtcgcg cttcgttttc cgccacatcc gtccgcaaac
    12121 gtaaatgcca gcccatactt aatgccagtt cagtaaaacc attaccaatc tgctctggca
    12181 gcaccacgca gaaaaatccc tcttcggtaa tgcactccgc cgcacaggtc agcaacgatg
    12241 ggtgatcaag cgtagtagta tagcgagcct gttcccgttg aggtgtcgag cactctactc
    12301 cctgctgata gtaaggtggg ttactgatga ttaaatcgaa gcgtactgtc tgctgtgtga
    12361 tccactgctg aatatccgcc gtatggacgt taatccgctc tgcccacggg gactggttga
    12421 tattttcctg cgcctgcgcg gcagcttcac tttccagttc aactgcatca atcatcacgc
    12481 tgtcatcggt tcgctgcgcc agcattaatg ccagcaaccc gctccccgcg ccgatatcaa
    12541 ggcaacgttt taccccagcc accggtgccc atgcgcccaa taaaatacca tccgttccca
    12601 ctttcatcgc acagcgatcg tgagcaacaa aaaactgttt aaaagtaaat ccattacgac
    12661 gaagcacgga tgtagactgt gacatgaaaa taaaaccttg caggaaaaac ggcgatagca
    12721 ccgggtgaga acaatacccg agaagcgata tccatacaaa cagatgaaga ttgcagccgt
    12781 aacgtctata atcagcgccc cacacagagg tagaacatga ctgtaacgac tttttccgaa
    12841 cttgaactcg acgaaagcct gctggaagcc ctccaggata aaggtttcac tcgcccgacc
    12901 gccattcagg ctgccgccat tccgcctgcg ctcgatggcc gtgatgtact cggttctgcg
    12961 ccgacaggca ccggtaaaac ggcggcgtat ctgctgccag cgttgcagca cctgctcgat
    13021 ttcccgcgta agaaatccgg tccgccgcgt attttgatcc tcaccccaac tcgcgagctg
    13081 gcgatgcagg tggccgatca tgcccgcgaa ctggcgaaac atacgcatct ggatatcgcc
    13141 accatcaccg gcggcgtagc ctatatgaac cacgcggaag tgttcagcga aaatcaggac
    13201 atcgtggtcg ccacgaccgg acgtctgctg caatacataa aagaagagaa cttcgattgc
    13261 cgcgcggttg aaacgctgat cctcgacgaa gcagaccgta tgctggatat gggcttcgcc
    13321 caggatattg aacatattgc tggcgaaacg cgctggcgta aacagaccct gctcttttcg
    13381 gcaacgctgg aaggcgatgc gattcaagac tttgccgagc gtctgctgga agatccggtg
    13441 gaagtttctg ctaatccttc cactcgtgag cgcaaaaaaa ttcatcagtg gtattaccgc
    13501 gccgatgacc tggaacataa aaccgcattg ctggtgcatc tgttaaaaca gccggaagcg
    13561 acccgctcaa ttgtgtttgt gcgtaagcgt gagcgtgtgc atgagctggc aaactggctg
    13621 cgcgaagcgg gcatcaacaa ctgctatctc gaaggtgaga tggtacaagg caagcgtaac
    13681 gaagcgatca agcgtttgac cgaaggtcgc gtaaacgtac tggttgcaac cgatgttgct
    13741 gcgcgcggta tcgacattcc tgacgtcagc cacgtcttta acttcgatat gccgcgcagt
    13801 ggcgatactt atttgcaccg tatcggacgt accgcgcgcg ccggtcgtaa aggcaccgca
    13861 atttcgctgg tagaagccca tgaccatctg ctgctgggca aagttggtcg ctatattgaa
    13921 gagccaatta aagctcgcgt aattgatgag ttacgcccga aaacgcgtgc accaagcgag
    13981 aagcagaccg gcaagccatc gaagaaagta ctggcaaaac gcgccgagaa gaaaaaagct
    14041 aaagagaaag agaagccgcg ggtgaaaaaa cgccatcgcg ataccaaaaa tattggtaag
    14101 cgccgtaaac caagcggaac gggcgtgcca ccgcaaacga cagaagagta atctcaatgc
    14161 cgggtttaag cccggcatta tttttatccc ggcaaaaaac aatcttcaac acactgaata
    14221 aaagtgtgca tcgccgggct aaccgctttt ccggcatggt gagcgcacat tgccgtaatg
    14281 gtctgcgagt gttcgccaaa aggcaattca attaattctc cgctctcaag ctctttttcc
    14341 accgcaaagc ggggcaagta actgacaccg atattcgccg cgacacaacg cttgatactt
    14401 tcaatgctta acagctcaat ggtgttttcc accgtgatcc gtcgctggcg cagcgtgctc
    14461 tcaaatatct gccggaagac acattgcggt tcgttgataa taaagctaca ggcgttatgt
    14521 cttcccggct cagtaaaatc gacatctgca atttgtggtg aagccaccag caccagcgat
    14581 tgttcaccca actctcgtcg gttcagggca tcatcattcc ctacgcgata aaagacgccg
    14641 acatcggcct catcattcag tagcgcatca cgaatcacgt aacagttcag cgactgcaac
    14701 gataaacgca cttttggtgc gcgttgccga aaacgctgca acacctgcgg cattcggtaa
    14761 gagagtaacg tttcacccga aacaacgcgt agttccccgt ccggatccga ctcctttttg
    14821 gcagcctcac gaagggtatc catcactctg gtaagttcat agatgtgcgg cagcagcttt
    14881 ttcccttcgc gggtaaggca cattcgtcga ccaattttct caaataactg tactgagaac
    14941 tcctgctcaa gctgctgaat atgaaaagtc actgtcgatt gtgtgcagca caatttttgc
    15001 gaagctcgca aaaaggaacc ctcttccacc acggttttaa gcgtaataaa acggcgcaga
    15061 tccataaccc caaacctatc gaaaatatcg aatttagaat gtaaaaacat tcattttttt
    15121 aaatattccg tgtcgggtac tgtctaccaa aacagaggag ataacaagtg acaccgacgc
    15181 ttttaagtgc tttttggacc tacaccctga ttaccgccct gacgccagga ccgaacaata
    15241 ttctcgctct tagctcagcc actacgcacg gctttcgtca gagtacccgc gtactggcag
    15301 ggatgagtct gggatttttg attgtcatgc tgctgtgtgc aggcatttcg tattcactgg
    15361 cagtgattga tccggcagcg gtacaccttt tgagttgggc aggagcggca tacattgtct
    15421 ggctggcatg gaaaatcgct accagtccaa caaaggaaga cgggcttcag acaaaaccga
    15481 tcagtttttg ggccagcttt gctttacagt ttgtgaacgt caaaatcatt ttgtacggcg
    15541 ttacggcact ttcgacgttt gtcctaccgc aaacgcaggc gttaagctgg gtagttggcg
    15601 tcagcgtttt gctggcgatg attgggacgt ttggtaatgt gtgctgggcg ctggcggggc
    15661 atctgtttca gcgattgttt cgccagtatg gtcgccagtt aaatatcgtg ctcgcgctgc
    15721 tgctgatcta ttgcgcggta cgcattttct attaattaaa aaagcggaag aagtcgccct
    15781 cttccgctta gtaacttgct acttaagcct tacaggcttt cagtaaaggt acgagcaata
    15841 acgtcgcgct gctgttccgg agtcagagag ttaaagcgaa ctgcatatcc ggatacacgg
    15901 atggtcagct gcggatattt ttccggatgc ttaactgcat cttccagagt ttcacgacgc
    15961 agaacgttaa cgttcaggtg ttggccacct tcaacgcgaa cttctggttt cacttctact
    16021 ggaacttcac ggtactcaat atcacccagt ttgcttactg caaccacttc gtcttctgca
    16081 aaacctgctt tcgcaacgat gcaacgcgct tcgccttttt cgctgtccag cagccagaaa
    16141 gagttcagca gatcgtcgtt agcggcttta gtaatctgga tacctgtaat catgtgatgc
    16201 ctccccggca aaattatttg atttgttcag cctgtcgcgg ccaattggta aaaccattgt
    16261 tgcttgagtg tatatatact cctcaaacac ccttgaatct ttgatttaaa tcaataaaaa
    16321 ccacacatca agtgtggtcg caaatggatt ttattgtttt acatcaactt atgcgggtgt
    16381 gaaattttac caatttacat ttttttgcac tcgtttaagt ctaaaaaatg agcatgattt
    16441 tgttctgtag aaagaagcag ttaagctagg cggattgaag attcgcagga gagcgagatg
    16501 gctaacgaat taacctggca tgacgtgctg gctgaagaga agcagcaacc ctattttctt
    16561 aatacccttc agaccgtcgc cagcgagcgg cagtccggcg tcactatcta tccaccacaa
    16621 aaagatgtct ttaacgcgtt ccgctttacc gagttgggtg acgttaaagt ggtgattctc
    16681 ggccaggatc cttatcacgg accgggacag gcgcatgggc tggcattttc cgttcgcccc
    16741 ggaatcgcca ctcctccgtc actgctgaat atgtataaag agctggaaaa tactattccg
    16801 ggcttcaccc gccctaatca tggttatctt gaaagctggg cgcgtcaggg cgttctgcta
    16861 ctcaatactg tgttgacggt acgcgcaggt caggcgcatt cccacgccag cctcggctgg
    16921 gaaaccttca ccgataaagt aatcagcctg attaaccagc atcgcgaagg cgtggtgttt
    16981 ttgttgtggg gatcgcatgc gcaaaagaaa ggggcgatta tagataagca acgccatcac
    17041 gtactgaaag caccgcatcc gtcaccgctt tctgcgcatc gtggattctt tggctgtaac
    17101 cattttgtgc tggcaaatca gtggctggaa caacatggcg agacgccgat tgactggatg
    17161 ccagtattac cggcagagag tgagtaaatt tgcgggaaaa tgccggatgg cagagttgcc
    17221 acccggctga tttatcaggc tttattctga cgccaccatt caccaagcaa aacgcctgtt
    17281 gcaacagaga tattcagccc ggcaacgttg cccgtaccgt caatcttcac gcgcagatcg
    17341 ttcggatcgc gtgcggcatc cggtaaccct tcatattcct gacccagcac cagtaccatt
    17401 ttcgctggca ggctggtttt gaacagcggt ttaccctgct cgctggaagt tgtcactacg
    17461 gtgtaacccg cctgacggaa atcatccagc acgttaacaa tgttgtcgcc agtaatcggc
    17521 tgaacgtgtt ctgcgccgcc ttctgcggta cggatagccg cacccgactc cagcagtgcc
    17581 gcatcctgca ccacaacgcc tttcacgccg aagtgcgcgc agctgcgcat catgccaccc
    17641 aggttatgcg ggttagattc gttttccagt gccagaacgc aatcctgcgc acctgcctgg
    17701 cttacccact gctgcacggt tgtgccatta cgctttttga tcaggaagca aacgccgccg
    17761 tgatgttccg tgcctgacgc ttttgtcaat tccgcttcat ccaccacatg gtacgctttg
    17821 cggtttgccg ccatccagcg caaggcttct ttaaaacgtg gcgttacgct ctggataaac
    17881 caggcgcgaa caatcgcttc cggacggctc tggaacagtg cctgacatgc attttcgccg
    17941 tagacgcggg tttcttccgc acgctgacga cgcaacactt ccggatcaat aaaactttta
    18001 ccactgatac caccgtgatc ggccttttcc ggcgtctcat cacccggcgc gcgggaaacc
    18061 gtgcgccacg gcgactcttc ccacttgcgg tcacggggct gactctgttt gtcatcgcgg
    18121 gcggggcgac ggccaccgtc agcacgagat tttcctggac gcccgccccc tttcccggta
    18181 cgcgggttgt gggtacgttt atcagaatca tcatcactgc ggacatacat cactttgacc
    18241 ttgccgcttt tacctttcat ttcatcgttc atgcttttct ccaccagcgc tgcgcgaagc
    18301 gcgcagatta cccgaagtcc gcgcggttcg ccatgatttc gtaccaaagc ctgcgactat
    18361 catacctatt gaataaaaca gattgttgtc tggaacaatg tccccgataa tatgtaacat
    18421 attagaaaca taccggcgtc gttgccgata agtctcctta ctcatcccga ggttagttat
    18481 gaataccgtt tgtacccatt gtcaggccat caatcgcatt cccgacgatc ggatcgaaga
    18541 tgcggcaaaa tgcggacgct gcggtcacga cttgtttgac ggagaggtga ttaatgcgac
    18601 cggtgaaacg ctcgacaaat tgctgaaaga tgatctacca gtggtgatcg acttctgggc
    18661 accgtggtgc ggcccctgcc gtaatttcgc accaattttt gaagatgtcg cgcaagagcg
    18721 tagcggtaaa gtgcgctttg tgaaagtgaa taccgaagct gaacgtgaat taagcagccg
    18781 ctttggtatt cgcagtattc cgacgatcat gattttcaaa aacggtcagg ttgtcgacat
    18841 gcttaatggc gcagtgccga aagcgccgtt cgacagctgg ttgaacgaat ctctttaatc
    18901 ttaccggggc gcatcttgtg ccccgttttc tcctctgcga caatggcgtt ttttcgacgc
    18961 tctcttatga ccgaaaacgc tgttctccag ttacgcgccg aacgtattgc gcgcgcaaca
    19021 cgtccttttc ttgcccgcgg taatcgcgtt cgtcgctgcc aacgctgtct tttgccagag
    19081 aaattatgtc tctgttcaac aatcacgcca gcggaagcaa aaagtcgctt ctgtttgctg
    19141 atgttcgaca ccgagccaat gaagcccagt aataccgggc gtctgattgc tgatattttg
    19201 cctgataccg ttgcgtttca atggtcgcgt accgaaccct cgcaggattt gctggatctg
    19261 gtacaaaacc cggattatca gccaatggtg gtctttcccg cctcgtatgc tgatgagcaa
    19321 cgggaagtga tcttcacacc acctgccggt aagccaccgc tgtttatcat gctcgatggt
    19381 acctggccgg aagctcgcaa gatgtttcgt aaaagtccgt atctggataa tcttcccgtc
    19441 atttccgtcg atctttcccg gctttctgcc tatcgcctgc gtgaagccca ggctgaaggc
    19501 caatattgta ctgccgaggt agccatcgca ctgttagata tggccggcga taccggggcg
    19561 gcagcagggt taggcgagca ttttacccgc tttaaaacac gctatctggc aggaaaaacg
    19621 caacatctgg gtagcatcac agcagaacag ttagaaagcg tttaaaatca ttcggtcact
    19681 tctgcgggag accggtatga gtcagcgagg actggaagca ctactgcgac caaaatcgat
    19741 agcggtaatt ggcgcgtcga tgaaacccaa tcgcgcaggt tacctgatga tgcgtaacct
    19801 gctggcggga ggctttaacg gaccggtact cccggtgacg ccagcctgga aagcggtgtt
    19861 gggtgtgttg gcctggccgg atattgccag cttgcccttt acacccgacc ttgcggtttt
    19921 atgtaccaat gccagccgta atcttgctct tctggaagag ctcggcgaga aaggctgtaa
    19981 aacctgcatt attctttccg ccccggcatc gcaacacgaa gatctccgcg cctgcgccct
    20041 gcgccataac atgcgcctgc ttggaccaaa cagtctgggt ttactggctc cctggcaagg
    20101 tctgaatgcc agcttttcgc cggtacctat taaacgcggc aagctggcgt ttatttcaca
    20161 atcggctgcc gtctccaaca ccatcctcga ctgggcgcaa cagcgtgaga tgggcttttc
    20221 ctactttatt gcgctcggcg acagcctgga tatcgacgtt gatgaattgc ttgactatct
    20281 ggcacgcgac agtaaaacca gcgccatcct gctctatctc gaacagttaa gcgacgcgcg
    20341 acgctttgtt tcggcggccc gtagtgcctc gcgtaataaa ccgattctgg tgattaaaag
    20401 cggacgtagc ccggcggcac agcgactgct caacacgacg gcaggaatgg acccggcatg
    20461 ggatgcggct attcagcgtg ccggtttgtt gcgggtacag gacacccacg aactgttttc
    20521 ggcggtagaa acccttagcc atatgcgccc gctgcgtggc gaccggttga tgattatcag
    20581 caacggtgct gcgcctgccg cgctggcgct ggatgcctta tggtcacgca atggcaagct
    20641 ggcaacacta agcgaagaaa cctgccagaa actgcgcgat gcactgccag gacatgtggc
    20701 aatctctaac ccgctcgatc tgcgcgatga cgccagcagc gagcattatg tcaaaacgct
    20761 ggacattctg ctccatagcc aggattttga tgcgctgatg gttattcatt cgcccagcgc
    20821 cgctgctccc gcaacagaaa gtgcgcaagc attaattgaa gcggtaaagc atcatccccg
    20881 cagcaagtat gtttctctgc tgacgaactg gtgcggcgag cactcctcgc aagaggcacg
    20941 acgtttattc agcgaagccg ggctgccgac ctaccgtacc ccggaaggaa ccatcactgc
    21001 ttttatgcat atggtggagt accggcgtaa tcagaagcaa ctacgcgaaa cgccggcgtt
    21061 gcccagcaat ctgacttcca ataccgcaaa agcgcatctt ctattgcaac aggcgattgc
    21121 cgaaggggct acgtcgctcg atacccatga agttcagccc atcctgcaag cgtatggcat
    21181 gaacacgctc cctacctgga ttgccagtga tagcaccgaa gcggtgcata ttgccgaaca
    21241 gattggttat ccggtggcgc tgaaattgcg ttcgccggat attccacata aatcggaagt
    21301 tcagggcgtc atgctgtacc tgcgtacagc caatgaagtc cagcaagcgg caaacgctat
    21361 tttcgatcgc gtaaaaatga cctggccgca ggcgcgggtc cacggcctgt tggtgcaaag
    21421 tatggctaac cgtgctggcg ctcaggagtt gcgggttgtg gttgagcacg atccggtttt
    21481 tgggccgttg atcatgctgg gtgaaggcgg tgtggagtgg cgtcctgaag atcaagccgt
    21541 cgtcgcactg ccgccgctga atatgaacct ggcccgctat ctggttattc aggggatcaa
    21601 aagtaaaaag attcgtgcgc gcagtgcgct acgcccattg gatgttgcag gcttgagcca
    21661 gcttctggtg caggtttcca acttgattgt cgattgcccg gaaattcagc gtctggatat
    21721 tcatcctttg ctggcttctg gcagtgaatt taccgcgctg gatgtcacgc tggatatcgc
    21781 gccgtttgaa ggcgataacg agagtcggct ggcagtgcgc ccttatccgc atcagctgga
    21841 agaatgggta gaattgaaaa acggtgaacg ctgcttgttc cgcccgattt tgccagaaga
    21901 tgagccacaa cttcagcaat tcatttcgcg agtcaccaaa gaagatcttt attaccgcta
    21961 ctttagcgag atcaacgaat ttacccatga agatttagcc aacatgacgc agatcgacta
    22021 cgatcgggaa atggcgtttg tagcggtacg acgtattgat caaacggaag agatcctcgg
    22081 cgtcacgcgt gcgatctccg accctgataa catcgatgcc gaatttgccg tgctggttcg
    22141 ctcggatctc aaagggttag gcttaggtcg acgcttaatg gaaaagttga ttacctatac
    22201 gcgagatcac ggactacaac gtctgaatgg tattacgatg ccaaacaatc gtggcatggt
    22261 ggcgctggcc cgcaagctcg ggtttaacgt tgatatccag ctcgaagagg ggatcgttgg
    22321 gcttacgcta aatcttgccc agcgcgagga atcatgagta aggtactgga aatgttgacc
    22381 acttaatcgg gactggtgtt attattgccc gcttatgtcg tctgcattgc acagaggacc
    22441 cttcaatgaa cagagaagaa atgcactgtg atgttgtcaa aatttaagcg taataaacat
    22501 caacaacacc ttgcccaact acccaagatt tctcaatcag ttgatgatgt cgatttcttt
    22561 tacgctcccg ccgacttccg ggagacgctg ctggaaaaaa tagccagcgc gaagcagcgc
    22621 atttgcattg tcgccctgta tctcgaacag gatgacggtg gcaaaggcat tctgaacgcg
    22681 ttgtatgaag ctaaaaggca gcgtccggaa ctggatgtgc gggtgctggt cgactggcat
    22741 cgtgcacaac gtggacgcat tggcgctgcg gcatctaaca ctaacgctga ctggtactgc
    22801 cgcatggcgc aggaaaatcc gggcgtagat gttccagttt atggcgttcc aatcaatacc
    22861 cgtgaagccc ttggcgttct gcactttaaa ggctttatca tcgacgatag cgtactttat
    22921 agcggtgcca gcctgaacga tgtttacctg catcagcacg ataaatatcg ctacgaccgt
    22981 tatcacctta tccgtaaccg taagatgtca gacattatgt ttgaatgggt tacacagaat
    23041 attatgaatg gccgcggcgt taatcgtctg gatgatgtta atcggccaaa aagcccggaa
    23101 atcaagaacg atattcgtct gttccgccag gagctgcgtg atgccgctta tcatttccag
    23161 ggcgatgccg acaacgatca gctttccgta acgccgctag tggggctggg gaaatcgagt
    23221 ctgttgaaca agaccatttt ccatcttatg ccttgtgccg agcagaaact aaccatctgt
    23281 acgccatact tcaacctgcc agcaatcctt gtgcgcaata ttatccagtt gctgcgcgaa
    23341 gggaaaaagg tcgaaattat tgttggtgat aaaaccgcga acgacttcta cattccggaa
    23401 gatgaacctt tcaagataat tggcgcattg ccttatctct atgagatcaa tctgcgtcgt
    23461 ttcctgagcc gtttgcagta ttacgtcaat actgaccagc tagtggttcg gttatggaaa
    23521 gatgacgaca acacctatca cctaaaaggg atgtgggttg atgataagtg gatgttgatc
    23581 accggtaata acctgaaccc gcgcgcctgg cgtctggatc tggaaaacgc cattttgatc
    23641 cacgatccac aactggagct tgcgccgcag cgagagaaag aactggagct gatccgcgag
    23701 cataccacca tcgttaagca ctatcgcgat ctgcaaagta ttgccgatta tccggtgaag
    23761 gttcgtaaac tcatccgccg tttgcgccgt atccgcatcg accgattaat tagccgcatc
    23821 ctgtaatcac aaccccgtcc tgtacggggt ttgttttttg gaggccacgt tttgcgtatt
    23881 ctttttgtct gttcgctatt gttactttct ggatgcagcc atatggctaa cgatagctgg
    23941 agcgggcagg ataaagctca acactttatc gcctcggcga tgctttccgc cgccggaaat
    24001 gaatattcac agcatcaggg gatgagccgg gatcgcagtg ccatgtttgg attgatgttc
    24061 tctgtcagtt tgggggcgtc aaaagagctt tgggatagcc gccccgaagg gagcggctgg
    24121 agctggaagg atttggcctg ggatgtcgcc ggtgcaagca ccggctatac cgtctggcaa
    24181 ctgacccgtc actaaagacg catccccttc cctttgcgat gtagcatcaa agaaaccaga
    24241 aacgccacca cggccatcaa ggtcacatac cagaagaagg ctgtttccat tcctattgat
    24301 ttcagcgaca acgctacgta ctccgccgaa ccaccaaata tagcattagc gaccgcatat
    24361 gacagaccaa cgcctaatgc gcgaacctgt gccgggaaca tctcagcctt cagtattcca
    24421 ctgattgatg tataaaaact cactatcagc agggcacaca tcaccagacc aaaagcggca
    24481 taaggcgagg aaacgttttg caatgctgag agaataggaa cggtaaaaat ggctgccagt
    24541 gaaccgaaac ataacattga ggtacggcga ccaatcttat ccgacagcgc gccaatgagt
    24601 ggttgaataa gcatgaatac aaacaatgcg gcagtcataa tgccactcgc cacgttggca
    24661 tgcattcccg cagtatttac cagatacttc tgcatataag tggtgaaggt atagaaacaa
    24721 agggagcccg cagcggtaaa accgagaacc atgatgaatg cacggcgatt gcgccataat
    24781 cctttcagag atccagcttc ttttaaagcg cgcgtttctt gttgcgaagt ttcatctaac
    24841 tgacgacgta accacaacgc cacaacagct aacacagctc ctaacgcgaa aggaatacgc
    24901 catccccact ctctgagtgc agcgtcttcc atggtgtgtt gtaaaaccac gacaaccagt
    24961 agggctagca gttgtccgcc gatcaacgtc acatactgaa atgatgcgta aaaacctttg
    25021 cgcccttcaa cggcaacttc actcatatag gtggcgctgg tgccatattc tccgccaacg
    25081 gataatccct gaaataaacg cgcgagaagc aataatgccg gagcccacgt accgattgtt
    25141 tcataaccag gcaaacaggc gattaccagt gagccgaaac acatcataca caccgatagc
    25201 agcatcgatt ttttgcgacc atgtttatcg gctatgcggc caaatagcca accgcctatt
    25261 gggcgcatga ggaatcccgc agcaaaaaca cctgctgttt gtagtagttg agtcgtcgtg
    25321 ttcccggaag ggaagaagat gtgggcaaag tagagtgaac agaacgagta gacatagaaa
    25381 tcgaaccact cgaccagatt acctgaagag gcccccacaa tcgcccaaat gcggcgacgt
    25441 gtatcactac ttgtcagttt gctgtctgcc gttacagtac tttcagccat gccattatgt
    25501 ctcctgccgt aatccgatgc ttttgtcggt cgcttttgtt tatttctttt ggtaaagaaa
    25561 atgttataca tttgttgcgt gtcattatgc aaccttaacc atgaatttag ttagcaggaa
    25621 aatggttatt gaggagctta aggataaatt tctggtgagg agaacacgta tggaggtggg
    25681 caagttgggg aagccgtatt cgctgctgaa tctggcatat gtgggagtat aagacgcgca
    25741 gcgtcgcatc aggcattttt ttcaccaacg caaaaaggcc atccgtcagg atggcctttc
    25801 gctt
//
LOCUS       DAZKFU020000045        25476 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZKFU020000045 DAZKFU020000000
VERSION     DAZKFU020000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.045"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            107..1543
                     /gene="mdtQ"
                     /locus_tag="ACHOQQ_RS07350"
                     /old_locus_tag="pgaptmp_001470"
     CDS             107..1543
                     /gene="mdtQ"
                     /locus_tag="ACHOQQ_RS07350"
                     /old_locus_tag="pgaptmp_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGU4469740.1"
                     /translation="MNRDSFYPAIACFPLLLMLTGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSELFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1596..2357
                     /locus_tag="ACHOQQ_RS07355"
                     /old_locus_tag="pgaptmp_001471"
     CDS             1596..2357
                     /locus_tag="ACHOQQ_RS07355"
                     /old_locus_tag="pgaptmp_001471"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4469741.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2487..3065)
                     /locus_tag="ACHOQQ_RS07360"
                     /old_locus_tag="pgaptmp_001472"
     CDS             complement(2487..3065)
                     /locus_tag="ACHOQQ_RS07360"
                     /old_locus_tag="pgaptmp_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4469742.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKIIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            3235..3822
                     /locus_tag="ACHOQQ_RS07365"
                     /old_locus_tag="pgaptmp_001473"
     CDS             3235..3822
                     /locus_tag="ACHOQQ_RS07365"
                     /old_locus_tag="pgaptmp_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGU4469743.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            3996..4928
                     /gene="pbpG"
                     /locus_tag="ACHOQQ_RS07370"
                     /old_locus_tag="pgaptmp_001474"
     CDS             3996..4928
                     /gene="pbpG"
                     /locus_tag="ACHOQQ_RS07370"
                     /old_locus_tag="pgaptmp_001474"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGU4469744.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(4967..6682)
                     /gene="dld"
                     /locus_tag="ACHOQQ_RS07375"
                     /old_locus_tag="pgaptmp_001475"
     CDS             complement(4967..6682)
                     /gene="dld"
                     /locus_tag="ACHOQQ_RS07375"
                     /old_locus_tag="pgaptmp_001475"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4469745.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            6878..9175
                     /gene="bglX"
                     /locus_tag="ACHOQQ_RS07380"
                     /old_locus_tag="pgaptmp_001476"
     CDS             6878..9175
                     /gene="bglX"
                     /locus_tag="ACHOQQ_RS07380"
                     /old_locus_tag="pgaptmp_001476"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGU4469746.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSSQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9427..10344
                     /gene="osmF"
                     /locus_tag="ACHOQQ_RS07385"
                     /old_locus_tag="pgaptmp_001477"
     CDS             9427..10344
                     /gene="osmF"
                     /locus_tag="ACHOQQ_RS07385"
                     /old_locus_tag="pgaptmp_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGU4469747.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLGRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10351..11508
                     /gene="yehY"
                     /locus_tag="ACHOQQ_RS07390"
                     /old_locus_tag="pgaptmp_001478"
     CDS             10351..11508
                     /gene="yehY"
                     /locus_tag="ACHOQQ_RS07390"
                     /old_locus_tag="pgaptmp_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGU4469748.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKFGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11501..12427
                     /gene="yehX"
                     /locus_tag="ACHOQQ_RS07395"
                     /old_locus_tag="pgaptmp_001479"
     CDS             11501..12427
                     /gene="yehX"
                     /locus_tag="ACHOQQ_RS07395"
                     /old_locus_tag="pgaptmp_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGU4469749.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVVDYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            12432..13163
                     /gene="yehW"
                     /locus_tag="ACHOQQ_RS07400"
                     /old_locus_tag="pgaptmp_001480"
     CDS             12432..13163
                     /gene="yehW"
                     /locus_tag="ACHOQQ_RS07400"
                     /old_locus_tag="pgaptmp_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGU4469750.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13144..13251)
                     /locus_tag="ACHOQQ_RS07405"
                     /old_locus_tag="pgaptmp_001481"
     CDS             complement(13144..13251)
                     /locus_tag="ACHOQQ_RS07405"
                     /old_locus_tag="pgaptmp_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGU4469751.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(13311..14042)
                     /gene="mlrA"
                     /locus_tag="ACHOQQ_RS07410"
                     /old_locus_tag="pgaptmp_001482"
     CDS             complement(13311..14042)
                     /gene="mlrA"
                     /locus_tag="ACHOQQ_RS07410"
                     /old_locus_tag="pgaptmp_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGU4469752.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGYDIFPLGI"
     gene            14264..15949
                     /gene="btsS"
                     /locus_tag="ACHOQQ_RS07415"
                     /old_locus_tag="pgaptmp_001483"
     CDS             14264..15949
                     /gene="btsS"
                     /locus_tag="ACHOQQ_RS07415"
                     /old_locus_tag="pgaptmp_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGU4469753.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            15946..16665
                     /gene="btsR"
                     /locus_tag="ACHOQQ_RS07420"
                     /old_locus_tag="pgaptmp_001484"
                     /gene_synonym="yehT"
     CDS             15946..16665
                     /gene="btsR"
                     /locus_tag="ACHOQQ_RS07420"
                     /old_locus_tag="pgaptmp_001484"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGU4469754.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16712..17182
                     /locus_tag="ACHOQQ_RS07425"
                     /old_locus_tag="pgaptmp_001485"
     CDS             16712..17182
                     /locus_tag="ACHOQQ_RS07425"
                     /old_locus_tag="pgaptmp_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGU4469755.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17223..17684)
                     /locus_tag="ACHOQQ_RS07430"
                     /old_locus_tag="pgaptmp_001486"
     CDS             complement(17223..17684)
                     /locus_tag="ACHOQQ_RS07430"
                     /old_locus_tag="pgaptmp_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGU4469756.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(17809..19812)
                     /locus_tag="ACHOQQ_RS07435"
                     /old_locus_tag="pgaptmp_001487"
     CDS             complement(17809..19812)
                     /locus_tag="ACHOQQ_RS07435"
                     /old_locus_tag="pgaptmp_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGU4469757.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMAQRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSAGVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHMLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(19809..20945)
                     /locus_tag="ACHOQQ_RS07440"
                     /old_locus_tag="pgaptmp_001488"
     CDS             complement(19809..20945)
                     /locus_tag="ACHOQQ_RS07440"
                     /old_locus_tag="pgaptmp_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4469758.1"
                     /translation="MSELNDLLTNRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARNFDFKSTLRANLQHWHPQHSKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVNLTADVADPVELLMKVQ
                     LGGGTSIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(20938..23217)
                     /locus_tag="ACHOQQ_RS07445"
                     /old_locus_tag="pgaptmp_001489"
     CDS             complement(20938..23217)
                     /locus_tag="ACHOQQ_RS07445"
                     /old_locus_tag="pgaptmp_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HGU4469759.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFLGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQSWCWQWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHKLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESDGKPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHVPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLN
                     DDDFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(23228..24316)
                     /locus_tag="ACHOQQ_RS07450"
                     /old_locus_tag="pgaptmp_001490"
     CDS             complement(23228..24316)
                     /locus_tag="ACHOQQ_RS07450"
                     /old_locus_tag="pgaptmp_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGU4469760.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDAAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5979 a         6477 c         6928 g         6092 t
ORIGIN      
        1 tggatatgtc gttttattgc tactgatgta ttgccacgcg aaactaaaaa atatatcgca
       61 acacacggta atctccgcgc accttttctt acctgattat tcccccatga atcgtgattc
      121 cttttatcca gccatcgcct gttttccgct gttactgatg ctgaccgggt gtgcgcctat
      181 gcatgaaacc cgccaggcgt taagccagca aacgcccgct gcacaagttg ataccgcatt
      241 acccacggcg ctgaaaaatg gttggccaga cagccaatgg tggctggagt atcacgataa
      301 tcaactcact tcattaatta acaatgcact acagaacgca ccggatatgc aggtggctga
      361 acagcgcatt cagctagctg aagcccaggc gaaggcaatt gccactcagg atggtccgca
      421 gatcgacttt tctgcggata tggagcggca aaaaatgtcg gcagaagggc taatggggcc
      481 gtttgctctg aacgatccgg ccgcaggtac gaccggtccg tggtacacca acggaacttt
      541 tggcttaacg gcgggctggc atctcgatat ctggggaaag aatcgggcgg aggttactgc
      601 ccgcctgggt acggttaaag cacgggcggc ggaacgcgag caaacccgcc aattgctggc
      661 tggcagcgta gcccgcctgt actgggagtg gcaaacccag gcggcgttaa acacggtctt
      721 gcagcaaatc gaaaaagagc agaacaccat tatcgcgacc gatcgccagc tatatcagaa
      781 cgggattact tcttcagttg aaggtgtgga aaccgatatt aatgccagca aaacccggca
      841 gcagctcaac gatgtcgcgg ggaaaatgaa aattattgag gcacggttaa gcgcacttac
      901 aaatcaccag acaaagtcat tgaagcttaa accggtcgcg ttgccgaaag tggcaagcca
      961 gcttcctgat gaactggggt actccttact ggcccggcgg gcagatttgc aggcggcgca
     1021 ctggtacgtt gagtcatcgc taagcaccat tgatgcggca aaagcggcat tttatcctga
     1081 catcaacctg atggccttcc tgcaacagga tgcgttgcac ttaagcgagc tgttccgtca
     1141 ttccgcgcag caaatgggcg ttacggcagg cctgacgcta cccattttcg atagtggtcg
     1201 tcttaacgcc aatctcgata tcgcaaaagc cgaaagcaac ttgtctatcg ccagctacaa
     1261 caaagcggtg gttgaagcgg tgaatgacgt ggcgcgggca gccagtcagg ttcagacact
     1321 ggcggagaaa aaccagcatc aggcgcaaat tgagcgcgat gccttgcgtg tggtaggtct
     1381 tgcgcaggcc cgctttaacg cgggcatcat tgctggttcc cgcgtcagcg aagccagaat
     1441 ccccgcgctg cgtgagcggg ccaatggcct gttattgcaa gggcagtggc tggatgcctc
     1501 cattcaactc actggtgcgt tgggcggggg ttacaaacgc tgatgatatg tgacttctat
     1561 acttaacgtt tattcagcgt taagtggaga actcgatggc acaggtagcg attattaccg
     1621 cctccgattc ggggatcggc aaagagtgcg cgttattact ggcgcagcaa gggtttgata
     1681 ttggcattac ctggcactca gatgaagaag gggcaaaaga taccgcgcgt gaggtagtta
     1741 gccacggcgt acgtgcggag attgtgcagc tggatctcgg taaactgcca gaaggtgcgc
     1801 aggcgttgga gaaactcatt caacggctgg ggcgtattga tgtgctggtg aataatgcgg
     1861 gcgcaatgac caaagcgccg tttcttgata tggctttcga tgagtggcgc aagattttta
     1921 ccgttgatgt cgatggtgca tttttatgct cacaaattgc ggcccgccag atggtgaaac
     1981 aagggcaggg cggtcgcatc atcaacatta cgtcggtaca tgaacatacg ccgctgccgg
     2041 atgccagcgc ctacacagcc gctaaacatg cgctcggtgg gttaaccaaa gcaatggcgc
     2101 tggagctggt cagacataag attttggtga acgcagtcgc gcctggggcg atcgccacgc
     2161 caatgaatgg catggatgac agcgacgtga agcccgacgc ggagccttcg attcctctgc
     2221 ggcgttttgg cgcaacgcat gagattgcca gcctggtagc gtggctgtgc tcagagggcg
     2281 caaattacac caccgggcag tcgttgatag tggatggcgg ctttatgttg gcgaatccgc
     2341 agttcaaccc agaatagcgc agtgaggtgc gtggttattt caatgggtaa aattttggta
     2401 ggcatgataa gacgcacaag cgtcgcatca ggcatggtgc cccggatgtc ggatgcggcg
     2461 cgaacgcctt atccagggca atggttttac gcctgattat ccggcttttt cttcccgcgc
     2521 cgtttcagcc accagcgcac gcctaccacc agaaccacga ccagaatcaa ccagacccag
     2581 tgcttcaaat gctgatcgag attgtgcaac cacggcgcaa tcacctgacc accagcgtaa
     2641 ccgatagtgg taaaaatcaa cgcccaggca aatgcgccga gaatattcag cggcagaaag
     2701 attttcggcg gcagctggct ggcaccaatc agcgtcgggc caatcacccg aaagccatac
     2761 ataaagcgcg taccaatgac aaacagatac ggatggcgtt ggataatttt ctgcgcccgc
     2821 tcaattttat cctgatgttt cgagaaacgg cgtaacagct tgccgccaaa ccgccgcccg
     2881 cacagataaa gcacctggtc gccaatcatg ccgccaagcg ccacagaaag taccaccagc
     2941 gggaacttta atagcccctg atgcgccgca acgcctccca gcaaagtcac ggtttcacct
     3001 tccgccaggc taccgatcac cagcgcggca taaccatatt gtgagatgag tgtattgaga
     3061 tccataggcc agtctgtctc cctttggttg cctgtaagca tacaccctgg aaatcattca
     3121 cgctgaaaaa ggacgtacat cagacggtgc aaattacatg agcgaacaaa aaataatcac
     3181 aagctgactt atacttgaac tgtcaggttc atgaactcac aacaaggggg cggtatgagc
     3241 catgtctggg gactgttttc ccatcccgat cgtgaaatgc aggtgattaa tcgcgaaaac
     3301 gaaacgattt ctcaccatta cacccaccac gttttgctga tggcggcgat tccggtgatt
     3361 tgcgccttca ttggcactac acagattggc tggaattttg gcgatggcac tatcctgaag
     3421 ttatcctggt ttaccggact ggcgctggct gtcttgtttt acggcgttat gctggctggg
     3481 gttgcggtga tggggcgggt catctggtgg atggcgcgca attacccgca gcgtccgtca
     3541 cttgcgcact gcatggtctt cgcgggctac gttgcaactc cgctgttttt aagcggtctg
     3601 gtggcgcttt atccgctggt ctggttgtgt gcgctggtcg gcacggtggc actgttttac
     3661 accggttacc tgctttacct tggtattccg agcttcctga atatcaacaa agaggaaggc
     3721 cttagcttct ccagttcgac gctggcgatt ggcgtgctgg tactggaagt gctgctcgcc
     3781 cttaccgtta tactatgggg ctacggttat cgtttgttct agaaagttct ttgacgttgc
     3841 attgctggcg taaatgtcag caatgcagca tttcctcacg attctccttt gacgatcgca
     3901 ctttttgctc gttatgatgg cgtcgctagc ctcagtaaat ccttactccg gcggtgcgca
     3961 acccgtgcgc gtgaaccact atctgaatgc tcatcatgcc gaaatttcga gtttctttat
     4021 ttagcctggc cctgatgctg gctgtgcctt tggcaccgca ggccgttgcg aaaacggcag
     4081 ccgctaccac cgcttcacaa ccggaaattg cctccggtag cgcgatgatt gttgatctga
     4141 ataccaacaa agtgatctat tcgaaccacc cggatctggt gcgtccgatt gcgtctatca
     4201 gcaaattaat gaccgcgatg gttgtgctgg atgcgcgact gccgctggat gaaaaactaa
     4261 aagtggatat cagccagacg ccggagatga aaggggtcta ttcgcgcgta cgactgaaca
     4321 gcgaaatcag ccgtaaagat atgctgttgc tggcgttgat gtcttcagaa aaccgcgcgg
     4381 cggcaagcct tgcgcaccat tatcccggtg gttacaaagc ctttattaag gcgatgaatg
     4441 cgaaagcgaa atcgctcgga atgaacaaca cgcgctttgt tgaaccgacc ggactatcgg
     4501 tgcataacgt ttcaaccgcc cgtgacttaa ccaaactgct cattgccagc aaacaatatc
     4561 cgttgatcgg gcagttaagt accacccggg aagatatggc aaccttctct aacccgacgt
     4621 atacgctgcc gttccgcaat actaatcatc tggtgtatcg cgataactgg aatatccagc
     4681 taaccaaaac cggctttacc aatgcggcgg gccattgtct ggtgatgcgt acggttatca
     4741 ataataaacc ggtggcgctg gtagtgatgg atgcgtttgg caaatatacc cattttgccg
     4801 atgccagccg cctgcgtacc tggattgaaa ccggtaaagt gatgcctgtt ccggcagcag
     4861 cgttgagcta taaaaaacaa aaagccgccc aaatggcggc ggcggggcag acggcacaga
     4921 acgattaagt gaattaggga tggcgatact ctgccatccg tcatttttac tccacttcct
     4981 gccagttttt ccgcttactg gttttaccga tccccggatt catactgttg gtcggatcgt
     5041 tctcgcgata aaacttctgc aacgtctccg gtgctttata caaatgaccg acgttatgtt
     5101 cggcagggta ttgcgcgccg cgctgctgta gcagctccag catctgttct ttcaacgcat
     5161 gcacatccac gcctttttta acgatgtaat cctgatggaa gacatagcac ataaaatggc
     5221 cgtaatagag cttgtgcacc agctggctgt cgatctccgg cggtaaatgc tcataccact
     5281 cggtgtcgtt acgccgcaga gcgatatcca gcgccagaat gtcttcgact tcatcggaat
     5341 gcaccgcctg ataacgaatt gctgcgcccg cagcggcgaa acggtgtaaa aacgctttgc
     5401 tgccttcctc cggcgtacag acaaagaaat cgccctcggc ctgtttgaaa taatccacca
     5461 gccacgattt ggcttcacca acgccatcgc ccgccatttt taacagcaga tgatgctcgt
     5521 atttatcgcg ccagtttttc atgcgcggcg gtaaatggct ggggaacagg tgaccgaatt
     5581 tttgcatcgc acggtcagta aaatgcggac ggaagaattt cactttctcc agcatcgcat
     5641 cggtgcgtcc cttgagatta aagaagaacg gcatcttgtc ggtgcctaac ttatcaatca
     5701 tcaggaaggt gtctttgccg tatttttccg caatatcgta gatatcccgg tgcatatatt
     5761 ccccggcaac cggcagattt tcgaagttag ccagaatatg acggcggatt tcggtcagca
     5821 cttccggctg gttggtgccg atataaaaca cctgctgatt tttttccgct tcgaaggtat
     5881 caagacgtac tgcaaagacg gccagcttcc cggcgcaacc agaagattca aataaccgat
     5941 ccggatcggc gttataacgt gcgggcgtgt cggcttcaat atcacgaacg cggtggacat
     6001 agtcataatc gtgggcgtga cgaccatcgt gacgcacatc gtcatctttg atgcgatcgt
     6061 catccagctt gctaaggatc tgctccggcg tttcgcccag atcaatcccc agatggttca
     6121 ccagcgtcag tttgccatct tcatttatac gcgcgaataa cgacatttcg gtatacgccg
     6181 ggccacgctg caccagcgaa ccaccagagt tattacagat accgccgatg accgatgcgc
     6241 ctatacacga tgatccaatc accgagtgcg gttcgcgtcc cagcggtttg agggcttttt
     6301 ccagcgaata gagcgtggtg cccggatagg ccagcacctg ttcgcccttg ccgagaacgt
     6361 gcagcttgtc gagacgcagg gtgctgatga tcacgatatc gcgatcataa tcgttaccgt
     6421 ttggcgtcga tccttcggtc aggcctgtat tggcagcctg catcagaata attttgtcgg
     6481 cggtgacgca ggctttcagc acccgccaca attccagtag tgagccaggg aaaacgacag
     6541 ccagcgcgtc gccctgacca gaacggaagc ccttgcgata gcgggccgtt tttgcgggat
     6601 cggtgagcag gtgtgaatga ccgaccagac gggcaagttc attcaaaaag gctttattat
     6661 cagttgttgt catggaagac attttccact ccttgtggtg gcgaaaaaat atcacaaact
     6721 agaatagcgc cttgtatgac aattcagcga gtgaatattt tgaaaattca gacaataaac
     6781 tgagcattct ttttctctct ccatcatgct tatggcaaac tacggttttt gcaccatttt
     6841 cgctgccacg tcgggcaaca aaggaagaaa aatccatatg aaatggctat gttcagtagg
     6901 aatcgcggtg agtctggccc tgcagccagc actggcggat gatttattcg gtaaccatcc
     6961 attaacgcct gaagcgcggg atgcgttcgt caccgaactg cttaagaaaa tgacagttga
     7021 tgagaaaatt ggtcagctgc gcttaatcag cgtcggcccg gataacccga aagaggcgat
     7081 ccgcgagatg atcaaagacg gtcaggttgg agcgattttc aacaccgtaa cccgtcagga
     7141 tatccgcgcc atgcaggatc aggtgatgga attaagccgc ctgaaaattc ctcttttctt
     7201 tgcttacgac gtgctgcacg gtcagcgcac ggtgttcccg attagcctcg gtctggcctc
     7261 gtcttttaac ctcgatgcag taaaaacggt cggacgtgtc tctgcttatg aagcggcaga
     7321 tgatggcctg aatatgacct gggcaccgat ggtcgatgtc tcgcgcgatc cgcgctgggg
     7381 acgtgcttcc gaaggttttg gcgaagatac gtatctcact tcaataatgg gtaaaactat
     7441 ggtggaagcg atgcagggta aaagcccggc agatcgctac tcggtgatga ccagcgtcaa
     7501 acactttgcc gcttacggcg cggtagaagg cggtaaagaa tacaacaccg tcgatatgag
     7561 ttcgcagcgc ctgtttaatg attatatgcc gccgtacaaa gcggggctgg acgcaggcag
     7621 cggtgcggta atggtggcgc tgaactcact caacggcacg ccagccacct ccgactcctg
     7681 gctgctgaaa gatgttctgc gcgatcagtg gggttttaaa ggcatcaccg tttccgatca
     7741 cggtgcaatc aaagaactga ttaaacatgg cacggcggca gacccggaag atgcggtgcg
     7801 cgtggcgctg aaatccggaa tcaacatgag catgagcgac gagtactact cgaagtatct
     7861 gcctgggttg atcaaatccg gcaaagtgac gatggcagag ctggacgatg ctgcccgcca
     7921 tgtactgaac gttaaatatg atatggggtt gtttaacgac ccatacagcc atctcggtcc
     7981 gaaagagtct gacccggtgg ataccaatgc cgaaagccgc ctgcaccgca aagaagcgcg
     8041 tgaagtggcg cgcgaaagcc tggtgttgct gaaaaaccgt ctcgaaacgt taccgctgaa
     8101 aaaatccgca accattgcgg tggttggccc gctggcagac agcaagcgtg acgtgatggg
     8161 cagctggtcc gcagccggtg ttgccgatca atccgtgacc gtgctgaccg ggattaaaaa
     8221 tgccgtcggt gaaaacggta aagtgctgta tgccaaaggg gcgaacgtta ccagtgacaa
     8281 aggcattatc gatttcctga atcagtatga agaagcggtc aaagtcgatc cgcgttcgcc
     8341 gcaagagatg attgatgaag cggtgcagac ggcgaaacaa tctgatgtgg tggtggctgt
     8401 ggtcggtgaa gctcagggga tggcgcacga agcttccagc cgtaccgata ttactattcc
     8461 gcaaagccaa cgtgacttga ttgcggcgct gaaagccacc ggtaaaccgc tggtgctggt
     8521 gctgatgaac gggcgtccgc tggcgctggt gaaagaagat cagcaggctg atgcgattct
     8581 ggaaacctgg tttgcgggga ctgaaggcgg taatgcaatt gccgatgtat tgtttggcga
     8641 ttacaacccg tccggcaagc tgccgatgtc cttcccgcgt tctgtcgggc agatcccggt
     8701 gtactacagc catctgaaca ccggtcgtcc gtataatgcc gacaagccga acaaatacac
     8761 ttcgcgttat tttgatgaag ctaacggggc gctttatccg ttcggctatg gtctgagcta
     8821 taccactttc accgtctctg atgtgaaact ttctgcgccg accatgaagc gtgacggcaa
     8881 agtgacggcc agcgtgcagg tgacgaacac cggtaaacgc gaaggggcca cggtagtgca
     8941 gatgtacttg caggatgtga cggcttccat gagtcgtccg gtgaaacagc tgaaaggctt
     9001 tgagaaaatc accctgaaac cgggcgaaac tcagaccgtt agcttcccga ttgatatcga
     9061 ggcgctgaag ttctggaatc aacagatgaa atatgacgcc gagcctggca agttcaatgt
     9121 ctttatcggc actgattccg cacgcgttaa gaaaggcgag tttgagttgc tgtaatgctt
     9181 tagtaagggc gcgacgtttg cgccctttgc ctgatgttca tatcttaact tgctgaattt
     9241 tcgccctttg taggccggat aaggcgctcg cgccgcatcc ggcaggaaac gccggaccga
     9301 atatgcctct taagtattta tctgattctg caactgaatc cgtccgctca ggctaactct
     9361 gtcattatca actatgcttt tctcttaatt cgctgaaaac aacagtaaaa gaatgaggaa
     9421 agcatcatgc cactctcaaa ggtctgggca ggttcactgg ttatgttggc agccgtgagc
     9481 ctgccgctac aggcagcttc tcccgttaaa gtcggttcaa aaatcgatac cgaaggcgcg
     9541 ctgctcggca atatcatttt gcaagtactc gaaagccacg gcgtaccgac ggtgaataaa
     9601 gtgcaactcg gaacgactcc tgtggtgcgc ggggcgatta cttccggtga actggatatc
     9661 tatccggaat ataccggcaa tggcgctttc ttctttaaag atgaaaacga tgcggcatgg
     9721 aaaaacgcgc agcaaggtta cgagaaagtc aaaaaactcg atgcagagca aaacaagtta
     9781 atctggctga cgcctgcacc ggcgaataac acctggacca tcgccgtgcg tcaggatgtg
     9841 gcagagaaaa acaaactcac ttcgcttgcc gatctcggtc gttatctgaa agagggcggc
     9901 accttcaaac tggcagcctc ggcagagttt atcgaacgcg ccgatgcgtt acccgcgttt
     9961 gaaaaagcct acgactttaa actcgatcag gatcagttac tgtcactggc tggcggcgac
    10021 acggcggtaa cgattaaagc cgctgcccaa caaacttctg gcgttaatgc cgcaatggct
    10081 tacggcactg acggtccggt cgcggcgctg gggctgcaaa ccttaagcga tccgcaaggc
    10141 gtgcaaccta tctacgcgcc tgcaccagtg gtgcgtgaat cggtgttgaa ggagtatccg
    10201 caaatggcac agtggctaca gccagtcttc gccagcctcg atgaaaaaac attgcagcaa
    10261 ctgaatgcca gcattgcagt tgaaggactg gatgccaaaa aagtggctgc cgactacctg
    10321 aaacaaaaag ggtggacgaa gtaatttccc gtgacttatc tacgtatcaa tcctgttctg
    10381 gcgctgctgc tgttgctgac ggcaatcgca gcggcgctgc cgtttatcag ttacgcgcct
    10441 aatcgtttag tctcgggtga agggcgacat ctctggcagc tgtggccgca aacgatttgg
    10501 atgctggtgg gcgttggttg cgcctggctg acagcctgtt ttattcccgc taaaaaaggc
    10561 agcatttttg cactcattct ggcgcaattc gtcttcgtat tgctggtgtg gggagcagga
    10621 aaggcggcga cccaactggc gcaaaatggc agtgcgctgg cgcgtaccag cctcggcagt
    10681 ggtttctggc tggctgcggc gctggcattg ctggcctgta gcgatgccat ccgccgaatc
    10741 tccacgcatc cgctgtggcg ctggttgttg catatgcaga ttgccattat tccgctgtgg
    10801 ttgctgtact ccggcacgct taacgatctc tcactaatga aagaatacgc caaccgtcag
    10861 gatgtgtttg acgacgcgct ggcacaacat ctgacgttgc tgtttggtgc ggtgctgcct
    10921 gcgttagtga ttggtgtgcc gttgggcatc tggtgctatt tttccactgc gcggcagggg
    10981 gcaatttttt ctctgctcaa tgtcattcag accgtgcctt cggtggcgct ctttggcctg
    11041 ctgattgcgc cgcttgccgg actggttacg gcctttccgt ggctggggaa gttcggcata
    11101 gcaggaaccg gaatgacacc cgcactgatt gcgctggtgc tctatgcctt gctgccgctg
    11161 gtgcgcggcg tggtagtagg cttgaaccag atcccgcgcg atgtgctgga gagcgccaga
    11221 gcgatgggga tgagcggggc gcagcgattc ctgcatgttc agttaccgct ggcgttaccg
    11281 gtatttctgc gcagcctgcg ggtggtgatg gtgcaaactg taggcatggc ggtgattgcg
    11341 gcgttaatcg gcgcaggcgg ttttggtgcg ctggttttcc aggggctgct aagcagcgcc
    11401 attgatttag tgttgctggg ggtgatcccg gtaattgttc tggcggtgct gatcgatgcg
    11461 ctgttcgatt tacttatcgc actgttgaag gtgaaacgta atgattgaat ttagccatgt
    11521 cagcaaactg ttcggcgcac aaaaagccgt taacgatctc aatctcaatt ttcaggaagg
    11581 gagtttttcg gtgctgattg gcacatctgg ttccggcaaa tccaccaccc tgaaaatgat
    11641 taaccgcctg gtggaacatg acagcggaga gatccgcttt gccggagaag aaattcgctc
    11701 gctgccagta ctggagttgc gccgccgtat gggctatgcc attcaatcta ttggcctgtt
    11761 tccccactgg agcgtggcac aaaacattgc caccgtgccg caattacaaa aatggtcgcg
    11821 ggcgaggatt gacgatcgta tcgacgaatt aatggcgcta ctggggctgg agtcaaattt
    11881 acgtgagcgt tatccacatc agctttccgg tggtcagcag caacgtgtgg gagtggcgcg
    11941 cgcactggct gccgatccgc aagtcttact gatggatgaa ccttttggcg cactggaccc
    12001 ggtaacgcgc ggcgcgttgc aacaagagat gacgcgcatt caccgtttgc tggggcgcac
    12061 tattgtgctg gtcactcatg atattgatga ggcgctacgg ctggcagaac atctggtatt
    12121 gatggatcac ggtgaagtgg tgcagcaggg gaatccgctg acgatgctga ctcgtccggc
    12181 gaatgatttt gtccgccagt tttttggacg tagtgaactg ggtgtgcgcc tgctttcgtt
    12241 acgtagtgtg gtggattacg tgcgtcgcga agaacgagca gaaggtgagg cactggcaga
    12301 agagatgacg ctacgcgatg cgctctctct gtttgtcgcg cggggatgcg aggtgctgcc
    12361 ggtggtgaac acgcagggcc agccttgcgg cacgctgcat tttcaggatc tgctggtgga
    12421 ggcgtaagcg tatgaagatg ttgcgcgatc cgctgttctg gctcattgct ttgtttgtgg
    12481 cactgatttt ctggctgcct tacagccagc cgctgtttgc tgccttgttc ccacaactgc
    12541 cacgacccgt ttatcagcaa gaaagttttg cagctctggc actggctcat ttctggctgg
    12601 tgggaatttc gagtttgttt gcggtgatca ttggtactgg tgccggaatt gctgtcactc
    12661 gcccgtgggg cgcggaattt cgcccactgg tggaaactat tgccgccgtt ggacagactt
    12721 ttccgcccgt cgcggtgctg gcgatcgccg ttccggtgat cgggtttggt ctgcaaccag
    12781 cgattatcgc cttgatcctt tacggtgtgc tgcccgtcct gcaggcgaca cttgccgggc
    12841 tgggagcgat tgatgccagc gtgacagaag ttgcgaaagg tatgggaatg agtcgtggtc
    12901 agcgactgcg taaggtcgag ctaccgctgg cggctccggt gattctggcg ggcgtgcgaa
    12961 cttcggtgat tatcaacatt ggtacggcga cgatagcctc aacggtaggg gccagcacgc
    13021 tgggtacgcc catcatcatc gggcttagcg gatttaatac cgcgtatgtg atccaggggg
    13081 cgttactggt ggcactggcg gcgatcatcg cagaccgcct gtttgaaagg ctggtgcagg
    13141 cgcttagcca gcacgcaaaa taaaggtata acctgcgagc atgacgccac caattccgcc
    13201 taacgccata aacaggaaca gggcgatgac cccaatttta gctatgcgca taatgcactc
    13261 cttatgttaa cgaaaggatt gtacagtaaa gcgcatttgt taacgaatca ttaaatgccg
    13321 agtgggaaaa tatcatagcc ttgttcttgc caactggtga gttgctgctg ttgggcggag
    13381 gttcgatttt caccgcacca caccagcaat gtacggcctt cgaacagttc agggcgtagt
    13441 tgattgagtg agtgggcgag gacatcaatg cgccagcctt gttgactggc aatccagccc
    13501 tccagccaca gacgggtggt atcgtgaata ttccagccaa ccaccagcgc atctttaccc
    13561 tgttttttac gtgccgaagc cagacaaatg gcgatgtagt tgatcagtac gccgtcgagg
    13621 atcgccagca gcgcctggag agtcggttgt tggcactgaa gccgtcggcg cagaggaata
    13681 aacagatgtg tggtgagcgt ctgggcgggg taatcctgac cgcgctcttt gatccacgtt
    13741 cgcaggctat gcagattgcc gctttgcagg taggtcagta atgtttcttg ctgatcgcgc
    13801 cagccgttct gcacatcaac attttcatta ctgagcagca ttttaacttt gctgacctgc
    13861 acgccgttgt cgatccagcg tttgatctcg cggatccggt caatatcggc atcgttgaac
    13921 agccgatgac cgccgtctgt ccgttgcggt ttcagcaatc cgtaacgcct ctgccacgcg
    13981 cgtaacgtga cagggttaat atcacaaagc aacgccactt caccaattgt gtaaagcgcc
    14041 atcgtctcac ccttgctcgc gaggtcccgg tttaacttta gacgcagttt tgcgaaccag
    14101 gtagttttgc ccgttttttg tgcatttata gggtgatttt atttttgcca ggcgattttg
    14161 agtgatcgta ctcacgaatt ctcatttttc tgcaagagtt caaagaaagt taaacgcagg
    14221 caatgtatgt tacgcgtttt aaagggaagt gtggtttgcg ggtatgtacg attttaatct
    14281 ggtgttgctg ctgcttcagc agatgtgcgt ttttttagtc attgcgtggt taatgagtaa
    14341 aacgccatta ttcataccgt taatgcaggt cacggttcgt ctgccgcata aatttctctg
    14401 ctacatcgtc ttttccatct tctgcatcat gggcacctgg tttgggttgc acattgacga
    14461 ttctattgcc aatacccgtg cgataggcgc ggtaatgggc ggcttactcg gcggtccggt
    14521 cgtcggtggg ctggttggcc tgaccggcgg gttacatcga tattcgatgg ggggcatgac
    14581 cgcgctaagt tgcatgatct cgaccatcgt tgaaggattg ctcggcggcc tggtacacag
    14641 catcctgatc cgtcgcgggc gcactgataa agtctttaac cccattaccg ccggtgccgt
    14701 cacgttcgtc gctgaaatgg tgcaaatgtt gatcatcctg gcgatcgccc gaccttatga
    14761 agatgcggtg cgtctggtga gtaatattgc tgcgccaatg atggtcacca ataccgtcgg
    14821 cgcggcgctg tttatgcgta tattgctcga taaacgcgcg atgtttgaaa aatacacttc
    14881 agctttttct gccactgcgc tgaaagtggc ggcctcgacg gaaggcattt tgcgccaggg
    14941 gtttaacgaa gtgaacagca tgaaagtggc acaggtgctg tatcaggagc tggatattgg
    15001 tgcagtcgcg attaccgatc gagagaaatt gctggccttt accggaattg gtgacgacca
    15061 ccatttaccc ggcaaaccga tttcttcgac ttatacctta aaagcgattg aaaccggtga
    15121 agtggtctac gctgatggca acgaagtacc ttaccgttgc tctttgcatc cgcaatgcaa
    15181 actggggtcg acgctggtaa ttccgttacg tggtgaaaat cagcgggtga tgggcaccat
    15241 caaattgtat gaagccaaaa accgtttatt cagttcaatc aaccgcacgt tgggtgaggg
    15301 gattgcgcaa ctactttcgg cgcagatcct tgccgggcaa tatgagcggc aaaaagcgat
    15361 gctcacccag tcagagatca aactgcttca cgcccaggtg aacccccatt ttttgtttaa
    15421 tgcgcttaac accattaaag cggtgatccg ccgcgacagc gaacaggcca gccagctggt
    15481 gcagtatctt tccacttttt tccgcaaaaa cttaaagcgg ccttcggagt ttgttactct
    15541 cgccgacgaa attgaacatg tgaatgctta tctgcaaatt gaaaaggcgc gcttccagtc
    15601 gcggttgcag gtcaacattg ctattccgca agaattatcc cagcagcaat tgcccgcgtt
    15661 taccctgcaa ccgatagtgg aaaacgccat taaacatggg acatcacaac tgctggatac
    15721 agggcgagtg gcaatcagcg cccgacgtga ggggcaacat ttaatgctgg agatcgaaga
    15781 caatgccggt ttgtatcaac cggtaaccaa tgccagtggg ctggggatga atctggtgga
    15841 taagcgttta cgtgaacggt ttggcgatga ctatgggata agcgtcgcct gtgagcctga
    15901 tagttacacc cgaataacgt tacgactacc atggagggac gaggcatgat taaagtctta
    15961 attgtcgatg atgaaccgtt agcacgggag aacctgcgcg tatttttgca ggagcagagc
    16021 gatattgaaa tcgttggtga gtgttcaaac gccgtggaag ggatcggcgc ggtgcataaa
    16081 ctgcgcccgg atgtgctgtt tctcgatatc cagatgccgc gcatcagtgg tctggaaatg
    16141 gtggggatgc ttgacccgga acatcgcccg tatattgttt ttctcaccgc gtttgacgaa
    16201 tacgccatta aagcctttga agaacacgcc tttgattatc tgctgaagcc aattgatgaa
    16261 gcgcgactgg agaaaacgct ggcgcgtttg cggcaggaac gcagcaagca ggatgtttcg
    16321 ctgttaccag aaaatcaaca ggcgctgaaa tttatccctt gtactgggca tagtcggatt
    16381 tatttgctgc aaatgaaaga tgtggcattt gtcagcagtc ggatgagcgg tgtctacgtt
    16441 accagccacg aagggaaaga gggctttacc gaattgacat tacgtaccct ggaaagtcgt
    16501 acaccactac tgcgctgcca tcgtcagtat ctggttaacc tcgcgcattt acaggagatt
    16561 cgtctggaag ataacggcca ggccgagttg attttgcgta atggcttaac cgtgccggtc
    16621 agccgccgtt atctgaaaag cttaaaagag gcgattggcc tgtaaaagac tgctaaaatg
    16681 gctttttgcc tcatcaacac ctgaaggcct catgctaagt aacgatattc tgcgcagcgt
    16741 gcgctacatt ttgaaagcca ataataatga cctggtgcgt atactggcgc tgggtaatgt
    16801 cgaagccacc gcggaacaga tcgccgtctg gctacgtaaa gaagacgaag agggttttca
    16861 gcgttgtccg gacattgttt tgtcgtcatt cctcaatggc ctgatttatg aaaaacgcgg
    16921 caaggatgag tctgctccgg cactggagcc ggaacgtcgc attaataaca acatcgtgct
    16981 gaaaaaatta cgcatcgcgt tttcgctgaa aaccgatgac attctggcga tcctcaccga
    17041 acagcagttc cgcgtttcga tgccggaaat tacagcgatg atgcgcgcac cggatcataa
    17101 aaacttccgc gaatgcggcg atcaattttt acgttatttt ctgcgtggac tggcagcgcg
    17161 ccagcatgtg aagaaaagct aagacgggta tggcggccat gcgaaacatg gccgccgaca
    17221 gattatttca cttctttaaa accagcggct ttcatcacca gttccatttg cgccatagtg
    17281 ataccttttt tggcatcttc agcagaaacg ttgattcctg aaataccctg cagggcttta
    17341 aaatccactt tttccatatc gatagtcacg ttttcctgcg cgtaggtatc ggtataggtt
    17401 aatttttctt caacacccgc gatgtttttg tatttggcgc ttaacggctc aagtgtcttg
    17461 gcagcatctt ctttggtggt tgcaccaatg gaggcaaatt gaattttggt ttcagaagat
    17521 tgcttaagca ccttgtcacc tttgtagaca taggtaatgg caatttcagt gccgttcaga
    17581 ttggcgctga atttcttcga ttcttctttg tcaccgcagc cagcaagaga gaaaaccaga
    17641 acagatgcaa caacaaggga aaacagctta ttgaaagcct tcatgtaaaa ctccatttta
    17701 tttaatcaag gaactggtga ctctcaccag gggctatata ggatatgcct aataccgtgg
    17761 cgtgagcagt ccggaactgg agtagaactc ttagtaaaaa gcactatttc atccttgttg
    17821 ctgaagcatg gggaataatt gttcgcacag caaaacaccg ttattcatag cttctacccg
    17881 tgcctcgctt tctgtattac gaaattgtcc caacatatgt gccagccgat aaaaacccac
    17941 cgcggtgagg tcattcgcca gcaactctgc ctgaccaata gcactctgtt cctgatagcg
    18001 ccagccgtta tggagcagtt gaataagtaa tgcctggcag cgcatcagca actgatgagc
    18061 agtagacggc acaggcagaa cgccggcaga aggtagtgat gccaccacag gcgcagtttc
    18121 tgcgtccagc gcccaggcac gagtctttgt catcattacc tgtggttcca gtgtcaattg
    18181 cccatcaaca aaactgacaa agccagaaac cagacacacg gggtcgtctg tttgttgcaa
    18241 aagcgccgcc atgcgttcaa cggcataagg cgcgctggct gaggctggca gtgataacgt
    18301 cagcagatta tcttcacctt cgccgctgat tacctgcgca tccagcgtct ggcggctgct
    18361 atcccaaccg agcgaaatac actcagcgac cggcagaata aataagttat cgacctgatt
    18421 aagaggccgt atgcaggagg ggggacgctg gcgtaaatat tcccgcaaag ccacaatgcc
    18481 cggctggcgt aacggcgcgc tcaacattcg ccaggcatca ggcgacagcg gcacaacgct
    18541 gcttaagcgg ttgcgggtag ctaacagcag ctcgccatcg gcactgcgtt ttgctgcttg
    18601 tgaaacaatt tgcccacccg ccagtgcgcc agcctgaaaa ctaaacagcc gacgcgtagc
    18661 tgccggtgag ttttcctgtt cacttcgcgg ccaactgcgc gaaaggtgca aaatactgcc
    18721 ggtgtcggga tcggtaaacc agatgcgtaa accataatgc tcaatatcct gccagcaacg
    18781 catacctaaa gacaccagcc gcagatgatc aagctttgct tctccggcaa tgccagagcc
    18841 aacgaccgtg cgccacggca caggaggaac ttcaccaaca ctgtcgcgct gggccatctc
    18901 ttgtgcgcaa tttaatcgac tgtttaatgc cgcaagctga cgtaagcatt ctccggcatg
    18961 atagtggctg gcgcgggcgt ggaaggcatc aacgctggcg cgcagttgcc gtagcgattc
    19021 actcacccat cgccagttgc agcgttccgc tgcttgctgc gcgcggctga aagcggcctc
    19081 gtagtgaata agcggctggc tgatgccgcc cagccataat gcctggctta gttgctgaac
    19141 atattgacga cacgttttgc cttcttcgct ggcaaacggg tcgtcagatg atgtgacgtg
    19201 ttcgctgcgc atctgccaga ttaaatgagt caattccgct tgctgcgctt ttgcctggac
    19261 aaaggcctgt actgccagta cgacatgttc gcaaagtgtg ccttcaatac aatcacaacg
    19321 ggcgaaacga atgctgctac gggaataaaa acgcacatcg ctcatcggta agcgggcaga
    19381 gggaatttcg cccggcgtac agaacaactc aatggtgatg cctttagcga ccagcgcctg
    19441 tgcgcgtttg cgggtggcat cgggaagggt agccagttct tccagccaga ttgccggatc
    19501 ccattcctct tcttttcccg taggctgggc ggtagcacaa agtcgttgat aacttaacac
    19561 cagcatcacg cgatgacggc acatgccgct ggccccgcag ctgcactgag cctctttcag
    19621 tgcctgaccg ttcgccagct gggtacggac accgtcactg aaggtggcga ttaaagcgcc
    19681 gttctcatgg ctgatttccg ggacgttgcc attttccagt tccttaaggc tgcgcttaac
    19741 aaaaccggca ttgcttaacg ccgtcagtgc ctgcggtgtc agttctaata attccggacg
    19801 tagtgaattc atgactgaag attctccgca agccatgatg ccagctcgcc cggcgtcatg
    19861 gcggctattt gtgcgccgac attaaccagc gcctgggccg tatcgcggtc atagcaaggc
    19921 gttgctgtgc tatcgagcgc tgccagtccc agcactttga tgccgctctg gacacacttt
    19981 ttcacctgat gcgtcagcaa tgatgatgaa cccccttcgt aaaaatcgct cacgaggata
    20041 atgacgcttt ttgctggttg ttcaataagt tgccgcccat actccacggc actggcgata
    20101 ctggtcccgc cgcccaactg tactttcatt aataactcta ccggatcggc aacgtctgcc
    20161 gtgagattaa cgacgcttgt gtcaaacgcc accagatggg tacgaatgcc gggtaactgc
    20221 cacaaacagg ccgccatcac cgcagagtgg atcaccgaat cgaccatcga tccgctttga
    20281 tcaaccagta agaccagttg ccattgttca ctgtggcgct taatacggct gttaaagcgg
    20341 ggggattcga tatacaactt gctgtgttgt gggtgccagt gttgcaggtt ggcgcgtaga
    20401 gtacttttga aatcaaagtt tcgcgccagt ggaatagatg agcggcgacg gcgatcgcgg
    20461 acaccagaaa aagcctgacg aacttccttt gccagtcgag ccataatttc ttcaacaacc
    20521 tggcgcacta tccggcgggc ggcagccagt acttcggggt tcatcagatg tttggtgtgc
    20581 aaaacggcgc gtagcaggct ttcagaaggc tgcatacgtt ccagcacgtc gagattcgtc
    20641 accacatctt caatgccata gcgcagtacg gcatcgcttt ccagccgctc aatcacctgt
    20701 tgcggaaaca gcgtgtgaat actgttgatc cactcagggg tggtgagatt tgagccacct
    20761 aatccaccgg agcgttcacc acgctgtagc cgttcaggat cgcgcccata cagccactcc
    20821 agcgcgtggt ctatctgccg ggcgttgtca tccagcccac aaagcgtcgt ttctgccgct
    20881 tcgccaagaa ttaatcgcca gcgttgtagc tcacggttgg tcagaagatc gttcagttca
    20941 gacatggaaa actccccagt tttgcagcga tgccagtgcc tgctgttcga gttgttgatg
    21001 atgtgcaatg gcttgtggtg gacaatgcaa cggcatttgc agcgccgaaa cgggaagttg
    21061 cgccagttga taatgctcaa gcacctgatg cgccagcgtc ccgcgttctc gtggtggtag
    21121 ccaggccatc gccgcgcgta aatcgggcag ggcgttaata aaatcgtcgt cattcagttg
    21181 atttaaatga ctgctgaaac cggcgataaa tgccggctgg caggccagtt gatgacgggc
    21241 gagcgccagc aaaccgtgca gcgcctcgcc ggattgtgcc ggggataact gcgccagcat
    21301 tgtcagcgcc gcttcggcac tggcgttggg atgttccaga cgcatcagtg cgccaagagc
    21361 cgcgccgcga tccagcgccg ggacatgaat tgcctgactg cggcgttcca gtaaggccac
    21421 cgccgcagaa agagaaacgc cgggtaaatt aacgccgcta tgtagatcgc gcaggatatg
    21481 gcaaagcgcc tgccagctat gcaggtgagc gtgaaactcc ttttcatccg gtttgccgtc
    21541 ggattcacac agccacagcg tgcgatcgat agccgcacat aacgtcgtct gcaatatctg
    21601 cgcgccttgc ataccgctga tttcatctaa ccgccataag gcataaagga cttccagcgc
    21661 ctgccccatt tcggcaaatt gattttcctg ggcgattaat aatgtgagtt gctccagtaa
    21721 ttgttgactg aaggacgcca gacccgctaa cgccgcctgg ctaagacatg tggtgatact
    21781 gccgattcca cccgcgtcca gcatatcggc ttctaattta tggcgtgcgg cttcctggag
    21841 tgtggcacca aagcaggcgg cctcaatcaa tgccgcatgc tggctaagcg ggcgggttaa
    21901 tttccagcgt tcttcaccgt tgcctgcaag tgtcagtgtg cttccctgct ggcgtacaat
    21961 ccctgggatc tccagtattg ccagccgatg taacacctga ctttgcgcta gtccattggg
    22021 gttaaagcga tttaaggtaa gctccgccgg aagagagatt gcggtacgtt ccagttcgca
    22081 ggtgacatct tttggcagag gcggttgtgg cgtagaaggg gcaagtttac cgaatccgtc
    22141 acccgctaat gtgtctatca acgttagcag aatcggatcg gtgtcgggat gaataactcc
    22201 gcgatagctc cacggcaacg gcgcgttcag ggcttcttta atcagcgagc ctgctatggc
    22261 atccagccag tcagtgcgta acggtagttt atgtccgcgc aactgtgcca gcgccatcgc
    22321 atgcagatga gcggcagcca tatccgcggt cgaagcaggc aagtggtgtt ggcgcaaacg
    22381 ggtaagaacc gtttttagca gttgttctcc cgcctgctgt aagccccact gccagcacca
    22441 gctttgccag accggggcag gcattcctga aaggtatcct gccagcacat caaggcgctt
    22501 ttcactgtag ggtgtgagat aacaacctgt aacggcatct gccagcgagg gcaattctgg
    22561 cttgttaatg tcctgcgggc attcgcgcca catttttgcc agtgccggag cgtgccagcc
    22621 accgcagacg actaacacgt cgccattatt ttgctgcacc gcccatgcaa tccagcgagc
    22681 cataaaggct tcgcgctgac gattgagtgc atcgccgagg aaatcgcccc gcagttgggc
    22741 aaaatagtgc gccagtgcag agggtaatgc agtttgctgg ctttcatctt cgaacaagtg
    22801 atcccacagg gtatcgctgt tatccatgcg ggtggcacgc agcagcaagg tctggctttc
    22861 ctcttgcgta tcaggcgaat cgtcctcttc ttcactctgt gcccagcaag gcaaatcgat
    22921 gaagtaagtt tgtgcctgaa tacgacgagc ggcttgtagc gcctgccact ccggcgaaaa
    22981 ttcagcaaat ggcgtccagg caccacgccc gggggctgca ccgtcctgat actggcaata
    23041 actgtaaata gctaccggaa gctggtgggc taaaaacagt tcgtctaccc ggtcattaaa
    23101 atcagccggg ccttcaatca acacgtatcg tggccgctgg ctttcgatta acgatttcac
    23161 cagccgggcg caggccggac tatgatgccg gatgccgaca attaacggct cgctcatgca
    23221 tctttcctca cggcaggcgg tggcgggctt gataataagc ctgccagtga gcttctttat
    23281 gtgtggcgac acgctgttcg aagtaacggc gcagacgagc gcgatcttct tcgttatctt
    23341 tgacgatggt tccggcaatg cagtccacca gatcggctgg ctcacctgcg cgattcgcta
    23401 aaaaccaggc gcggacgccc acggcgtggg ccacgttcac ggcttcagcg gtggacataa
    23461 tcgctgttag tgtatccatt gaggttttct tttcgccgtt ggcacgcaga tcgcggaagg
    23521 tgcggaccag taactccagt accgcgtcag gcactttatg cggaataccg ctatgggcca
    23581 gcaaacgcgc cgaagcactg gcgacaagct ccagctcctg ggcgaaatcc ataatcggaa
    23641 agacagtttc aaaatcgaag cgacgcttta gcgcagcact catttcattg acgccgcgat
    23701 cgcgggtatt ggcagtggca ataatattaa agccttctcg cgcatagagc tgactggctt
    23761 caccagtgag ttccggcacc gtcatcaccc tgtcagaaag catacctaac agacagtctt
    23821 gcacttccag cggtgtacgc gttatctctt caaaacggac gattttgccg tcgcgcattc
    23881 cctggtaaag cggggcaggg accagcgctt ccgttgatgg accatgattg ataagcaggg
    23941 cgtagttcca gccgtacttg atttgatctt cagtagtaga tgcgccgccc tggatggtta
    24001 acccggcatc gccgctaata gcggttgcca gtaattcaga caggagagat tttgcggtgc
    24061 caggttcccc caccaacatc aaaccgcgcc cggtggcaag agtgactaac atacgttcga
    24121 cagcggaggg actaatcacc actttacggc taatattttg cgcgctatcg ccaaggataa
    24181 aagcacgggc cgcaggcaaa cttaactgcc aaccgggcgg gcaaggtgcg gcatcatttt
    24241 gttttaattt tgccagttca tcggcgtata acacagcagc aggcggacgc tgcagatgat
    24301 tattctgcgg tgacatatat tcatccctga aatgtattaa tgaaataaac gatattttac
    24361 gcgcaataaa tgtttggcgc attagttgtt tgagcgaaaa atatctggaa agcagatcac
    24421 agtaaataaa aaaaactgct ggtgaggcag gttcttttat ttactatttt caggcctaac
    24481 accacagcct gtgcaacatc gtgttccaac gaacgaatta taaccacggc tggaccccgt
    24541 tacaattaag ataatgagat ttataatcca gaccgttggg acgttgcgcc ggatcgaacc
    24601 tatttcaagt cctggctacc ggacgggtgg tgctgcaggc ggtgccctga tccggagtgc
    24661 aacatcgtgg tccaacatac gtaaaccagt atcgacaggc agtttaaggg taaggtgctg
    24721 gtgttgcgct ccttaataag gagagcggat ggacacgctg acacaaaagt taaccgtgct
    24781 cattgctgta ctggagctat tagtagctct gttacggttg attgatttgt tgaagtaacg
    24841 ggcaaattga aacgcatcta agggggaaaa ccgcttcccc ctttaataaa atgattactg
    24901 tttttattaa ttcatcatat aactcatgtt tatttataag ttcatcgatt ttatattcaa
    24961 cgcaccattt gaccgggatg tgaatattta tggtgggcta ttggcaaaaa atatctggaa
    25021 agtagatcgc agaaaataaa aaaacctgct ggtgaggcag gttcttttat ttactacagt
    25081 gacgctaact tgctgattgt gcaacatttc gtaccaacga ataaattata accatatcca
    25141 cactccattg caattcagtg aatgagattt ataatcctgc ccgttgggag gttgtgccgg
    25201 atcggaccaa ttttaagtcc tggctgctgg acgggtggtg ccgcaggcgg tgccctgatc
    25261 cggagagcaa cgtttcgtac caacatacgt aaaccagtat cgacaggcag tgtaagggta
    25321 aggtgctggt gttgcgctcc ttaataagga gagcggatgg acacgctgac acaaaagtta
    25381 accgtgctca ttgccgtact ggagttatta gtggctctgt tacggttgat tgatttgttg
    25441 aagtaacggg caaattgaaa cgcatctaag ggggaa
//
LOCUS       DAZKFU020000046        24731 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZKFU020000046 DAZKFU020000000
VERSION     DAZKFU020000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.046"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..565
                     /locus_tag="ACHOQQ_RS07455"
                     /old_locus_tag="pgaptmp_001491"
     CDS             <1..565
                     /locus_tag="ACHOQQ_RS07455"
                     /old_locus_tag="pgaptmp_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGU4469761.1"
                     /translation="LSGCGYNDIQTYDEQVNASWSEVLNQYQRRADLIPNLVASIKGY
                     SSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQQAQAQVTRTLGQLTIISER
                     YPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKFPAVLTAKVMDYTP
                     KKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            579..1250
                     /locus_tag="ACHOQQ_RS07460"
                     /old_locus_tag="pgaptmp_001492"
                     /pseudo
     CDS             579..1250
                     /locus_tag="ACHOQQ_RS07460"
                     /old_locus_tag="pgaptmp_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            1487..2635
                     /locus_tag="ACHOQQ_RS07465"
                     /old_locus_tag="pgaptmp_001493"
     CDS             1487..2635
                     /locus_tag="ACHOQQ_RS07465"
                     /old_locus_tag="pgaptmp_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4469762.1"
                     /translation="MRIFLLLLMLCSFQVFAERLPSVNDLTGTLTTEEQSVLNQQLQT
                     LEQQNHLQVAVLVTPTTGGKNIKLAASQSYGLYHWKPIGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPKQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLAPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASIKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSGRW"
     gene            2632..3534
                     /locus_tag="ACHOQQ_RS07470"
                     /old_locus_tag="pgaptmp_001494"
     CDS             2632..3534
                     /locus_tag="ACHOQQ_RS07470"
                     /old_locus_tag="pgaptmp_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4469763.1"
                     /translation="MIVMRLIFILLALWSLPGLAQQIAVPELRQQVTDITGTLRTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKERASRKGSSSSSSG
                     GGSSGGGFSGGGGSSGGGGASGRW"
     gene            complement(3594..4892)
                     /gene="eno"
                     /locus_tag="ACHOQQ_RS07475"
                     /old_locus_tag="pgaptmp_001495"
     CDS             complement(3594..4892)
                     /gene="eno"
                     /locus_tag="ACHOQQ_RS07475"
                     /old_locus_tag="pgaptmp_001495"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGU4469764.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4980..6617)
                     /gene="pyrG"
                     /locus_tag="ACHOQQ_RS07480"
                     /old_locus_tag="pgaptmp_001496"
     CDS             complement(4980..6617)
                     /gene="pyrG"
                     /locus_tag="ACHOQQ_RS07480"
                     /old_locus_tag="pgaptmp_001496"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGU4469765.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6845..7636)
                     /gene="mazG"
                     /locus_tag="ACHOQQ_RS07485"
                     /old_locus_tag="pgaptmp_001497"
     CDS             complement(6845..7636)
                     /gene="mazG"
                     /locus_tag="ACHOQQ_RS07485"
                     /old_locus_tag="pgaptmp_001497"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGU4469766.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7707..8042)
                     /gene="mazF"
                     /locus_tag="ACHOQQ_RS07490"
                     /old_locus_tag="pgaptmp_001498"
     CDS             complement(7707..8042)
                     /gene="mazF"
                     /locus_tag="ACHOQQ_RS07490"
                     /old_locus_tag="pgaptmp_001498"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGU4469767.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(8042..8290)
                     /gene="mazE"
                     /locus_tag="ACHOQQ_RS07495"
                     /old_locus_tag="pgaptmp_001499"
     CDS             complement(8042..8290)
                     /gene="mazE"
                     /locus_tag="ACHOQQ_RS07495"
                     /old_locus_tag="pgaptmp_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGU4469768.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNGITPENLHENIDWGEPKDKEVW"
     gene            complement(8368..10602)
                     /gene="relA"
                     /locus_tag="ACHOQQ_RS07500"
                     /old_locus_tag="pgaptmp_001500"
     CDS             complement(8368..10602)
                     /gene="relA"
                     /locus_tag="ACHOQQ_RS07500"
                     /old_locus_tag="pgaptmp_001500"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGU4469769.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10650..11951)
                     /gene="rlmD"
                     /locus_tag="ACHOQQ_RS07505"
                     /old_locus_tag="pgaptmp_001501"
     CDS             complement(10650..11951)
                     /gene="rlmD"
                     /locus_tag="ACHOQQ_RS07505"
                     /old_locus_tag="pgaptmp_001501"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGU4469770.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYSRAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            12008..14764
                     /gene="barA"
                     /locus_tag="ACHOQQ_RS07510"
                     /old_locus_tag="pgaptmp_001502"
     CDS             12008..14764
                     /gene="barA"
                     /locus_tag="ACHOQQ_RS07510"
                     /old_locus_tag="pgaptmp_001502"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGU4469771.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(14997..16337)
                     /gene="gudD"
                     /locus_tag="ACHOQQ_RS07515"
                     /old_locus_tag="pgaptmp_001503"
     CDS             complement(14997..16337)
                     /gene="gudD"
                     /locus_tag="ACHOQQ_RS07515"
                     /old_locus_tag="pgaptmp_001503"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGU4469772.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(16358..17698)
                     /gene="gudX"
                     /locus_tag="ACHOQQ_RS07520"
                     /old_locus_tag="pgaptmp_001504"
     CDS             complement(16358..17698)
                     /gene="gudX"
                     /locus_tag="ACHOQQ_RS07520"
                     /old_locus_tag="pgaptmp_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HGU4469773.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(17700..19052)
                     /gene="gudP"
                     /locus_tag="ACHOQQ_RS07525"
                     /old_locus_tag="pgaptmp_001505"
     CDS             complement(17700..19052)
                     /gene="gudP"
                     /locus_tag="ACHOQQ_RS07525"
                     /old_locus_tag="pgaptmp_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGU4469774.1"
                     /translation="MSSLSQAASRVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(19487..19936)
                     /gene="yqcA"
                     /locus_tag="ACHOQQ_RS07530"
                     /old_locus_tag="pgaptmp_001506"
     CDS             complement(19487..19936)
                     /gene="yqcA"
                     /locus_tag="ACHOQQ_RS07530"
                     /old_locus_tag="pgaptmp_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGU4469775.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(19954..20736)
                     /gene="truC"
                     /locus_tag="ACHOQQ_RS07535"
                     /old_locus_tag="pgaptmp_001507"
     CDS             complement(19954..20736)
                     /gene="truC"
                     /locus_tag="ACHOQQ_RS07535"
                     /old_locus_tag="pgaptmp_001507"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGU4469776.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(20736..21065)
                     /gene="yqcC"
                     /locus_tag="ACHOQQ_RS07540"
                     /old_locus_tag="pgaptmp_001508"
     CDS             complement(20736..21065)
                     /gene="yqcC"
                     /locus_tag="ACHOQQ_RS07540"
                     /old_locus_tag="pgaptmp_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGU4469777.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDVPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21687..22232)
                     /gene="syd"
                     /locus_tag="ACHOQQ_RS07545"
                     /old_locus_tag="pgaptmp_001509"
     CDS             complement(21687..22232)
                     /gene="syd"
                     /locus_tag="ACHOQQ_RS07545"
                     /old_locus_tag="pgaptmp_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGU4469778.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            22300..23148
                     /gene="queF"
                     /locus_tag="ACHOQQ_RS07550"
                     /old_locus_tag="pgaptmp_001510"
     CDS             22300..23148
                     /gene="queF"
                     /locus_tag="ACHOQQ_RS07550"
                     /old_locus_tag="pgaptmp_001510"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGU4469779.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGADIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGNVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            23260..24624
                     /gene="ppnN"
                     /locus_tag="ACHOQQ_RS07555"
                     /old_locus_tag="pgaptmp_001511"
     CDS             23260..24624
                     /gene="ppnN"
                     /locus_tag="ACHOQQ_RS07555"
                     /old_locus_tag="pgaptmp_001511"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGU4469780.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKHTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         6081 a         6500 c         5938 g         6212 t
ORIGIN      
        1 tttatcaggc tgtggatata acgatattca aacctatgat gagcaggtta acgcctcctg
       61 gtctgaggta ttgaatcaat atcaacgtcg cgccgatctt attcctaatc ttgtcgccag
      121 cattaaaggt tattccagcc atgaacgaga tgtactggaa gccgtaacat tggctcgtag
      181 ccaggctaat cgcgccagca gcgatcttca gcaaactccg ggagatgaac aaaaattaca
      241 agcctggcaa caggctcaag cgcaggtgac tcgcactctc ggtcagttaa cgataattag
      301 tgagcgttac cctgagttaa aatctcagga actctatcaa aatttaatgg tacagcttga
      361 aggaagtgaa aatcggattg ccgttgcgcg aggaagatat attaaagcca ttgaacaata
      421 taatgtcact atccgcaaat tccccgctgt gttaaccgca aaggtcatgg attacacacc
      481 aaagaaaaat tatttgccgg atgatgtcgc cgcagtaagt aaagccccaa caatagattt
      541 tagccagaat gctaatgctc attaagcggg gaagttctat gcgaaatttt atcttcctta
      601 tggctttctt ctgttcatct gtgtttgcca cacaaattcc agtacctgaa tcacccaagt
      661 atgtgaatga cttaacgggc acattaacaa acagcgaagt aaacaccctg accaaccaga
      721 ttaaagccct gacgcaaaaa agccacgcac aactggtcgt gttattggtt gaaacaacag
      781 gcgatgaaac catcgagcag tacgcaacgc gggtattcga tagctggaaa ccgggtgata
      841 aagaccgcga tgatggcgtt ctactgttag tcgcctggca ggatcatacc gtgcgtattg
      901 aaatcggtta cggactggaa ggtattatta cggatgcgca gtccgggaaa attattcgca
      961 acagtattat tcctgcattt aaaaaaggcg acctggctgg cggtttacaa aaggggatca
     1021 gcgatattga aagtcgtctt acggggaata atttagcgac gatagcaccg gccgatcacc
     1081 ctttaccttt tagcggttgg tgggccttgt tagcctgggc gatagtactg acgtttattt
     1141 ctgccagagg atatatcaaa acgctgggag taatttgctt cgcagctatt gtactggcct
     1201 ttgttttacc tatagcaggt ttcagcggaa gctggggagt actcacaaca ttactctgct
     1261 tcgccacacc gttcctggct gtcgccatcg cttttacccc cttcggcaaa aaagttcggg
     1321 atttgatgcg tgatgccaac cagccatcac gccatacaag aagcaattct tcctcccact
     1381 attcatcatc gtcatcctct tcattcaaca atgacaattt cagcggtggt ggcggttcat
     1441 ctggtggcgg tggcgcttcc gggcgctggt aacaaaagga attgatatgc gtatttttct
     1501 gttacttctg atgctatgca gttttcaggt attcgctgaa cgattgcctt cagtgaatga
     1561 cttaaccggg acattaacga cagaagaaca aagtgtttta aatcaacaat tacagactct
     1621 cgagcagcaa aaccacttac aggtggccgt gttagttacg ccaaccacag ggggcaaaaa
     1681 cattaaactt gcagcctcgc agagctacgg gttatatcac tggaaaccca ttggagagaa
     1741 gcgatacggc gaaggtatct taattctcgt cgtttggccg gaagggctgg cgtcaatgaa
     1801 gattggacat ggactggaag agatgttgcc gccgaaacaa gctgcgcaaa ttgttcgtta
     1861 ccatatgcag ccagaattcg aaaaaaacaa tctctttgct ggattaacgg gcggaattga
     1921 aagcatcgca cagtttaccc atatcaaagc gaaccttagc cctttggatg ctttagccaa
     1981 tcatctgttt gctaatccgc aacgttcgct gccatgtctt gcctggacac tgctgatgat
     2041 cgttgccatc gttgtcctct ggcgatttgc cagccgccca cggcgtggca tatggatgat
     2101 atcgatgatt acccctatgg tctggttttt ctcctttcag gacgatgtca tcattcggcg
     2161 ggtatcggtc gtttgtttta gtttattgtt agcaacaacc tgcaagcagc gtctcgcggt
     2221 agtttttaat atgtgccgcg tatttcctat gatgctggcc ccaaaaaata aaaacgctaa
     2281 agtgaaaaaa caaaagaaga ctccgcaggt tcgtgaatca ggacgaaatt cattgtttgt
     2341 cgtgatgctg ataattattg tgggatggtg cctggtgttc gccagtatta aagatattgc
     2401 attcatctca acaattattg ggttaattga tcattttatc ggtccaataa ccattgctgg
     2461 catcgttctg ctgatcattg tggccaaatt tactggcaat cttaagctgg gatcaggaga
     2521 aaaaagcaac aggaaaacgg ggaatcgtaa tgctcactct cgctcttcgt cgagaaattc
     2581 tttccgcggt ggtggcggtt cttcaggagg cggcggttca tccggccgtt ggtgatcgta
     2641 atgcgcttaa tctttattct acttgctttg tggagccttc ccgggcttgc ccagcaaatt
     2701 gctgtgccgg agctgcgcca acaagtgact gatattactg gtacgttaag aacgtctgaa
     2761 caacaatcac tgacacagca gctgcaggat attacacata aaacacgggc gcaggtcgct
     2821 gtattagtag tgccgtccac cggagacgat tccattgaac aatatgctac aagggttttt
     2881 gatagctgga aactgggaga taagcagaga aacgatggta ttttacttct ggttgcctgg
     2941 gaggatcatg ctgttcgtat tgaggttggc tacgggctgg aaggggtggt taccgacctg
     3001 caagcggcga agattatcag ggatatattg atccctgcct tcaaaagtga tgacctgatg
     3061 ggtggattaa cactggcaag cgaaaatatc ggcgcgcttc tgttgaatgg tgaattaccg
     3121 gaagacaggg gggattatta cagtatcaat ccccctattc cattatcgct tgctgtaatt
     3181 atattgctgg cggtactttc atattttatt gtttttaccg atccgtcgaa tttaccctgg
     3241 atcactctca ctggcgccat ttacgggatg gtattcctct atgttgccga gccaggacca
     3301 tggaccaatt taatcgttgc ctgcggtatg ttaacgcctt ttgcgatcgt accactggtt
     3361 attttttggc ttatcgtcaa taaaaagcta cgcgccaaat acaaaaaact cagtaaagaa
     3421 agagcttcaa gaaaaggttc ttcttcatcc tcttccggtg gaggctcaag tggcggcgga
     3481 tttagcggcg ggggcggttc ttctggaggt ggcggcgcat ccggccgctg gtaactcaac
     3541 cataaaaaat gccagcctcc gggctggcat ttttaaatca gataaagtca gacttatgcc
     3601 tggcctttga tctctttacg accgttgtac ggtgcttttt cgcccagagc ttcttcgata
     3661 cgaatcagct ggttgtattt agcaacgcgg tcagaacggc tcatagaacc agttttgatc
     3721 tggcctgcag cagtaccaac agccaggtca gcgatggtag cgtcttcagt ttcgccagaa
     3781 cggtgagaga taactgcagt gtagccagcg tctttcgcca tcttgattgc agccagagtt
     3841 tcggtcagag aaccgatctg gttgaatttg atcaggatgg agttagcgat acctttttcg
     3901 ataccttctt tcaggatctt ggtgttggtt acgaacaggt cgtcaccaac cagctggatt
     3961 ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc agtcagattc gtccagaccg
     4021 tcttcgatag aaacgatcgg gtactgtttg gtcagttctt ccaggaagtg agtgaattct
     4081 tcagaggtga aagctttgtt gccttcgcca gccagaacgt atttaccgtc tttgtagaat
     4141 tcagaagccg cgcagtccat cgccagagtg atgtctttgc ccagttcata accagcagct
     4201 ttaacagctt cagcgataac agccagagct tcagcgttgg aacccaggtt cggcgcatag
     4261 ccaccttcgt caccaactgc agtgttcatg cctttcgctt tcagaacttt tgccagatga
     4321 tggaaaactt cagaacccat gcggatggct tctttcacag ttttcgcgcc aaccggctga
     4381 atcatgaatt cctggatatc gacgttgttg tcagcgtgct caccaccgtt gatgatgttc
     4441 atcatcggaa ccggcatgga gtatttgccc ggagtgccgt tcagttcagc gatgtgctcg
     4501 tacagcggca tacctttagc agctgcagca gctttggcgt tagccagaga tacagccagg
     4561 attgcgttcg cgccgaattt ggatttgttt tcggtgccgt ccaggtcgat catgatcttg
     4621 tcaatgccag cctgatcttt agcatctttg ccaatcagcg cctgagcgat cgggccgttt
     4681 accgcagcaa cagctttggt tacgccttta cccaggaaac gggatttgtc gccatcgcgc
     4741 agttccagag cttcacggga accagtagaa gcacctgacg gagcagctgc cataccgacg
     4801 aaaccaccct ccagatgtac ttcggcttca acagtcgggt taccacggga gtcgatgatt
     4861 tcacgaccga tgatttttac gattttggac attaggtttt cctcaagtca ctagttaaac
     4921 tgaaactcca gacaaacaac gcgtacccag ggtacgcgtt gccgttctaa cttttttact
     4981 tacttcgcct gacgtttctg gaactcgctg gcggctttca caaagcctgc aaacagcggg
     5041 tgaccatcac gtggagtaga agtaaactcc ggatggaact ggcaagccac gaaccacggg
     5101 tgattcggaa cttcgatgat ctcgaccaac tgatcatccc cggaacggcc cgcaacgcgc
     5161 agacctgcat cttcaatctg tttcaacagc atgttgttga cttcgtaacg gtgacgatga
     5221 cgctcaacaa ttgtcggcgc attgtacagc tggcgaacca agctatcgtc aaccaactgg
     5281 cactgctgtg cgccgagacg catggtaccg ccgagatcgc tcttctcgct acgaacttca
     5341 acgttgccgt tttcatcgcg ccactcggta atcagcgcca caaccgggta cttacagtct
     5401 ggcacaaatt ccgtagagtt ggcgttctcc atgttggcaa catggcgagc gtaatcaatt
     5461 aacgccacct gcatacccag gcaaatgccc agataaggaa tattgttctc acgcgcaaaa
     5521 cgcgcggtcg taatcatgcc ttctacgcca cgatagccga aaccgccagg tacgaggatt
     5581 gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa catcttgtga atcgatcagt
     5641 ttgatgttga cgctgacacg attcttcagc ccaccgtgtt tcagtgcttc gatcacggat
     5701 ttataagcat ccggcagttc aatgtacttg ccgaccatac cgatggtgac ttcacttacc
     5761 ggattcgctt cttcgaagat aacctgttcc cattcggaca gattcgcttc cgggcagttt
     5821 aagctgaatc gtttacaaat ataatcgtcc agcccctgag atttcaacag gcccggaatt
     5881 ttatagatgg aatcgacgtc tttcagagaa ataaccgctt tttccggaac attacagaac
     5941 aatgcaatct tcgcacgttc gttcgccgga acggcgcgat ctgaacgaca aatcaggatg
     6001 tcaggctgga taccgatgga gagcagctct tttacagagt gctgagtcgg tttggttttg
     6061 acttcaccag acgctgccat gtacggcacc agcgtcaggt gcataaacag agtgtgctca
     6121 cggccaattt caacagccat ctggcgaatc gcttcgagga acggcaggga ttcgatatca
     6181 cctactgtac cgccgatttc gaccagtact acgtcatgac cttcgccacc ttccagcacg
     6241 cgctctttga ttgcgttagt gatgtgcgga ataacctgta cggttgcgcc gaggtagtca
     6301 ccgcggcgtt ctttacgcag aacgtcagag tagatacgac ccgtggtgaa gttgttgcgg
     6361 cggctcattt tggtacggat gaaacgctcg tagtgcccca ggtccaggtc ggtttcagcg
     6421 ccgtcttcag taacgaacac ttccccgtgt tggattgggc tcatagtacc tggatcgacg
     6481 ttgatgtacg gatccagttt catgatggtc acattgaggc cacgggcttc aagaatggct
     6541 gcgagggagg ctgcggcaat gcctttaccc agagaggata cgaccccgcc ggtcacaaaa
     6601 atatagttcg ttgtcatgct gaacctgaga agttaggttg aaaagacgat ggaataacca
     6661 ggacgggaaa gcagtatacc cgaacatgac ctgtgccaca aactttcatt atccctcctc
     6721 ttcgccagcg cactattgaa atcaggagtg agaaaatagc ccctttgggg taaatgtttt
     6781 tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa atggcgcttg accgcgtaat
     6841 tcccttagag atcaatttcc tgccgtttta cctgttgcca gacttcttcc attgtttcga
     6901 ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat acgctccact tcgcgaaaac
     6961 gacgctcgaa tttttcgttc gctttttgca atgcgatttc cgcttttgtc cctaaatgac
     7021 gagccagatt gaccgtggca aacagcaggt cacccatttc ctcctccagt ttagcctggt
     7081 cgataacagc ctgccgtgcc tcgtacatca cctcgtcgat ctcttcgtag actttatcaa
     7141 ccactgggcc aagcgtcgtc cagtcgaagc caacgttggc gcaacgtttc tggatttttt
     7201 gcgcccgcat taaagccggt aaactacgag gaatatcgtc cagcgccgaa tgctgcgctt
     7261 tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc aagcacttca ctactgtttt
     7321 cggcagaact gtcagcaaaa acatgcggat ggcgacgctc taatttatcg ctaatcgcag
     7381 cgcaaatatc attaaagtca aagcgccctt cttcctgagc catttgggcg taaaacacca
     7441 cctggaacag cagatcgccg agttcaccac gcagatcgtc aaaatcttca cgggcgatgg
     7501 cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc aatggtggca aatgtctgct
     7561 ctttatccca cgggcagccg ttttccgggt cgcgcaggcg ctgcataata gtgagcaaac
     7621 ggtcgatttg attcattaga atgtcctgaa aattgcgggt ccgtcaggtg gaaacctgtg
     7681 accagaatag aagtgagtta gtaacactac ccaatcagta cgttaatttt ggctttaatg
     7741 agttgtaatt cctctggggc aactgttcct ttcttcgttg ctcctcttgc ccgccaggcg
     7801 atacttttta cctgatcagc taacgctacg ccatcacgtt cctgaccgga taaaacaact
     7861 tcgaacggat atccttttga ttgcgttgta caaggaacac acagacacat acctgttttg
     7921 ttgttgtaca tgaacggact caggacaaca gccggacgat gtccggcttg ctcgctacct
     7981 tttgtcgggt caaaatcaac ccaaatcaga tcgcccatat cgggtacgta tcggcttacc
     8041 attaccagac ttccttatct ttcggctctc cccagtcgat attctcgtgg aggttttccg
     8101 gcgtgatgcc gttgaccagt tcagcaagcg taaatacggg ctctttacgc actggctcaa
     8161 taattaattt gccatccacc aggtcaatct tcacttcatc atcaatattc agattgagcg
     8221 cctgcattaa cgtagccggg atccgcaccg ccggtgaatt tccccaacgc tttacgctac
     8281 tgtggatcat aaccctttcc tcaaaccgct atcatatgta gatacagtat atatcaatct
     8341 acattgtaga tacgagcaaa tttcggccta actcccgtgc aaccgacgcg cgtcgataac
     8401 atccggcacc tggttgagtt tacccagcac gcgacccagc acctgcaggt tgtaaatctc
     8461 aatggtcatg tcgatggtcg ccagttgctg tttggtgtcg ctacggctgg caacgccaag
     8521 cacgttcacc ttctcgttgg cgagaatggt cgtgatatca cgtaacaacc cactacgatc
     8581 attagccacc acgcggacca ccagcgaata tccggcggag tagctctcac cccataccgc
     8641 gtcaacaatg cgttctggcg catgggagcg cagttccgcc agttgttcgc aatcggcgcg
     8701 gtgtactgaa ataccgcgcc cctgggttat gaagccgaca atctcatctc caggaatcgg
     8761 ctggcagcag cgcgcgatgt ggtgcatcag gttaccgaca ccttcaacca ccacacgacc
     8821 gttatcttta ctgcggtttt gcggtgtgta gcttttttgc tgaagctgtt tcagcgcggc
     8881 ggcgtcctgc tcttcggcac tcggcttatt aaattgcgat tgcaggaagt tcaccatctg
     8941 attgagacgg atatccccgc caccaatcgc cgccagcaac tcgtcgacat cattgaagtt
     9001 gtaacgcggc agcagatgtt tttctgcttc tttcaggctg atccccagat gttccagctc
     9061 gtcgtcaagg atttgccgcc cagccagaat gtttttgtca cggtcctgtt tacggaacca
     9121 ggcgtgaatt ttcgaacgcc cacggctggt tgtgacgtaa ccgaggtttg ggtttaacca
     9181 gtcacggctg gggttcggct gtttctgggt gataatttca atctggtcgc ccatttgcag
     9241 ctggtaggtg aacggcacaa tgcgcccgcc aatttttgcc ccgatacagc ggtgtccgac
     9301 atcactgtgg atgtggtaag cgaagtccag cggcgttgat cccgcaggca aatcaacgac
     9361 atcacctttc ggcgtaaaga catacacccg gtcgtcaaac acctgactac gtacttcgtc
     9421 gagcatttcg ccggaatcag ccatctcttc ctgccacgca atcagtttac gcagccaggc
     9481 aatccggtct tcatgtcccg aacgtgcgcc gccagctgcc gcgccctctt tatatttcca
     9541 gtgcgcagca acacccaact ctgcatcttc atgcatctgt ttggtgcgga tttggatctc
     9601 aacggttttt ccacccggcc ccagaaccac ggtatgaata gactgataac cgtttggttt
     9661 cgggttagcg acgtaatcat caaactcatc cggcaggtgg cgatagtgtg tgtgcactat
     9721 ccccagtgcg gcataacaat cctgtaaacg ctcggcgaca atacgtaccg cacgcacatc
     9781 aaacagctca tcgaaggcga ggttcttttt ctgcattttg cgccagatgc tgtagatgtg
     9841 tttcggacga ccatacactt cagctttaac gccttcagct ttcatctcag cgcgcagatg
     9901 accgacaaac tcttcgatat agtgttcgcg gtcgagacgg cgttcatgca acagttttgc
     9961 gatgcgtttg tattcggtcg ggtggagata acggaagcag taatcttcca gctcccattt
    10021 cagttgccca atcccaagac ggttcgccaa cggcgcgtag atattggtgc actcttttgc
    10081 cgccagtacg cgttcatctt ccggcgcatc ttttacttca cgcagatgag caatacgctc
    10141 cgccagtttg atgaccacgc agcgaaaatc atcgaccatc gccaataaca tccggcgaac
    10201 gttatcgacc tgttcggagg aaacagaatc agtgtgcgtc gctttcagct ggcggatcgc
    10261 cgccatatca cgcacgccgt gaataaggtt aacgaccgac ttaccgacgc tctcacgcag
    10321 cacatcttcg ctgactacgt tggcatccgc cagagggaaa agcagcgccg cccgcagcgt
    10381 gtcaatgtcc atacttaatg tcgagaggat ctccaccatc tcaacaccac gccacaataa
    10441 cagactggca tccggatgcc cctgcgtctg ttgcagacaa tacgcccagg tttcggctaa
    10501 gcactcacac gacttctggc tggtaatacc cagacttgcg atccattttt ccggatcaaa
    10561 ttcaccagcc ttattgatat gtgcacttct taccgcaacc atcgtcctct cctttaggga
    10621 ccagacctgc cgaaatcggc aaatcgcaac tatttaacgc gcgagaaaag taccatcgat
    10681 tccagatgtc ccgtgtgtgg gaacatatcc agcatcgcca gtcgcgcaat ggtatatcct
    10741 gcttttaata acgcttcgct atcccgagcc agcgttgcag ggttacagga tacataaact
    10801 atacgaatag gttccagttt tataatttgc tgcataacac ctgcggcacc tgctcgcgcg
    10861 gggtccagca acactttatc gaagccgttt ttcgcccacg gctgctttgt gacatcttct
    10921 tcaagatttt catgataaaa cgtcacattc tgtaagccat taagacgcgc attctgctgg
    10981 cctttttcca ccagcgcagg aacaccttct acacccacca cactggctgc ttgtgtcgcc
    11041 aatggcagtg taaagttgcc cataccgcag aacagatcca gtacgcgatc ttcaggttgc
    11101 acatccagcc attccaacgc acgcgctacc attttttggt tcacacccgc attgacctga
    11161 ataaaatcgc gcgggctaaa agttaagcgc aacccatttg agtcatacca gggcatctca
    11221 ccagagacgg tttcgagtat ctcactatcg ggggcgagat acagatccag gccttcagaa
    11281 tgcgaaaagc gttccagttt ttcgcgatct gccgaactta gcggtgcggt atggcgcaaa
    11341 atcatcagcg tgccgctggt tgcctgtacc agttcaacat gaccaagatg gcgcatagct
    11401 tgtaagctgc ccaggcatgc cctgactttg ggcagcaatg cttcaagttg gggcactaaa
    11461 atggggcatt gtttaacgtc gacaatgtca ctggagcccg ctttgcgaaa ccccatctga
    11521 agttgctgtg ttttcggtaa gtaattcaga cttaaacgcg cgcgacggcg atagccccag
    11581 ggaacatcgg cgatcacttc agagacttcg tgtttcatta atcgggcgag tgccgcactt
    11641 ttgcttcgct gctgtaaatc cacgctggcg tgttgttgct gacagccgcc gcatacgcca
    11701 aaatgaggac agcgtggcgt ttcgcgttcc gggctatcgc ttaaccggcg tacgacttta
    11761 gcgcgggaat actgtttttt atcttcagta acagtaactt ccgcgttttc ctgcggcaat
    11821 aatccgggga taaatagcgt tttgccgtta tgtcgcgcca cgccctgacc aaaagagtcg
    11881 aggtcgttga ctgaaacggt tatgatctga cgcgtcgtcg tgcgtcgttt tgcagagtag
    11941 aattgcgcca ttggcgagac tttctcaatt taacagtgtg accttaattg tcccataacg
    12001 gaactccatg accaactaca gcctgcgcgc acgcatgatg attctgatcc tggcaccgac
    12061 cgtccttatt ggtttattgc tgagtatctt tttcgtcgtg catcgctata acgacttgca
    12121 gcgtcaactg gaagatgccg gtgccagcat tattgagccg cttgcagttt ctactgaata
    12181 tggcatgagc ctgcaaaatc gcgaatctat cggtcagtta ataagcgtac tgcatcgtcg
    12241 ccattccgat attgttcgcg cgatttcggt ttatgatgaa aataaccgac tctttgtcac
    12301 ctccaatttt catcttgatc cctcatcaat gcagctcggc agcaacgtgc cgtttcctcg
    12361 ccagctcact gtcactcgtg acggcgatat tatgatcctc cgcacgccga ttatttctga
    12421 gagttactcc cctgacgaat cgcccagtag cgatgccaaa aatagtcaaa atatgttggg
    12481 atatattgcg cttgagctgg atcttaaatc ggttcgcttg cagcaatata aagagatctt
    12541 tatttccagc gtgatgatgc tgttttgtat cggtattgcg cttatttttg gctggcgctt
    12601 aatgcgcgat gtaaccggtc cgattcgcaa catggtgaat accgtcgacc gcatccgtcg
    12661 cgggcaactc gacagccggg tggaaggatt tatgctcggc gagctggata tgctgaaaaa
    12721 cggtatcaac tcgatggcaa tgtcgctggc tgcttatcac gaagagatgc agcacaatat
    12781 tgaccaggcg acgtccgatc tgcgtgaaac gctggagcag atggaaattc agaacgttga
    12841 gttggatctg gcgaaaaagc gcgcccagga agcggcgcgt attaaatccg agtttctggc
    12901 taatatgtca cacgagctgc gtacaccact gaatggtgtt attggcttta cccgcctgac
    12961 gctgaaaaca gaattaacac caacgcagcg cgatcacctg aatacgattg aacgttcggc
    13021 aaataatttg ctggcaataa ttaatgatgt tctcgacttc tcaaaactgg aagcaggtaa
    13081 gctgattctg gaaagtattc cattcccact acgcagcacg ctggatgaag tcgttaccct
    13141 gctggctcat tcttctcacg ataaagggct ggaactgacg ctcaatatta agagcgacgt
    13201 gcctgataac gtgatcggcg acccactgcg attacagcaa atcatcacta acctggtggg
    13261 gaatgcaatt aaattcaccg agaatggcaa cattgatatt ctggtagaaa aacgtgcgct
    13321 gagtaatacc aaagtgcaga ttgaagtgca gattcgggat accggcattg gtattcctga
    13381 acgcgatcaa tcgcgcttat tccaggcctt ccgacaggct gatgccagta tttcccgccg
    13441 tcatggtggc accgggctgg ggctggtgat tacacaaaaa ctggttaatg aaatgggcgg
    13501 cgatatttcg ttccatagcc agccaaatcg cggttcaact ttctggttcc acattaatct
    13561 cgatctgaac ccgaacatta ttattgaagg gccatccacc cagtgcctcg ccggtaaacg
    13621 cctggcctat gtcgaaccaa actccgcagc agcgcaatgc acgctggata ttttaagtga
    13681 aacgccgctg gaagtggttt atagcccaac gttctccgcg ctgcctcccg cgcattacga
    13741 catgatgttg ttaggcatcg cggtgacctt ccgcgagccg ttaacaatgc aacatgagcg
    13801 attagcgaaa gcggtatcga tgaccgattt cctgatgctg gcacttcctt gccatgcaca
    13861 agtcaatgct gaaaaactca agcaagatgg tatcggcgcg tgtctgctga aaccattaac
    13921 acctacgcgc ctgctgcctg ccctgacgga attttgtcat cacaaacaaa acacgctttt
    13981 gcctgtaacc gatgaaagta agctggcaat gacagtcatg gcggttgatg acaaccccgc
    14041 taacctgaaa cttatcggcg cattgctgga agatatggtg caacatgtgg aactttgcga
    14101 tagcgggcat caggcggttg aacgggcgaa acagatgccg ttcgatttga tcttaatgga
    14161 tattcaaatg ccggacatgg atggcattcg ggcctgcgag ctcatccacc agctccccca
    14221 tcagcaacaa acgccggtta tcgcggtaac ggcgcatgca atggccgggc aaaaagagaa
    14281 gctgcttggc gcaggaatga gcgattatct ggcgaaaccg attgaagaag agcgattgca
    14341 taatttgttg ttgcgctaca agcctggcag cggtatttcg tctcgcgtcg tgacgcccga
    14401 agtcaacgaa attgtggtga acccgaatgc gaccctcgac tggcaactgg cactacgcca
    14461 ggcagcagga aaaaccgatt tagcgcgcga tatgctgcaa atgttactcg atttcctgcc
    14521 tgaagttcgc aacaaagttg aggaacagct ggttggagaa aacccggaag gcctggttga
    14581 tttgattcat aaactgcatg gcagttgcgg ctatagcggt gtgccgcgta tgaagaatct
    14641 ctgccaactg atcgaacaac agctacgtag cggtactaaa gaagaagatt tggagccgga
    14701 gctgctggaa ctgttggacg agatggataa tgtcgcgcgc gaagccagca aaattctcgg
    14761 gtaacggacg tcttgtcgag tagcaatcgg ctttttatga cgctggtttc agattggtga
    14821 caaagtgcct gtgtttatgc cggatgcggc gttaacgcct tatccggcct acaaaaccaa
    14881 ttaaattcaa taaattaaaa agcatgtagg cctgataagc gtagcgcatc aggcaatttt
    14941 gtgtttggca tcagtctcaa accggctcct gacagagccg gttttggttt tctgtcttaa
    15001 cgcaccatgc acgggcgctt gttatcgaac gtccagccag gaatcagata ctgcattccc
    15061 atcgcatcgt cacgcgcgcc aagcccgtgt ttctgataca gctcatgggc tttcatcact
    15121 tgatccatat cgatttctac acccagcccc ggtttttctg gcacctgtac cagcccgcct
    15181 ttgatctcaa acggttcttt ggtcaggcgc tgattgcctt cctgccaaat ccagtgcgta
    15241 tcaatagcag taattttacc cggtgcagcg gcggcaacat gggtaaacat cgccagggaa
    15301 atatcgaagt ggttgttaga gtgtgaaccc caggtcaggc caaattcatg gcacatttgc
    15361 gccacacgta ccgaaccttg catcgtccag aaatgcggat ccgccagcgg gatatcaacg
    15421 gattgtaacg agagcgtatg gcccatctgc cgccagtcgg tggcgatcat attggttgca
    15481 gtcggcaggc cagtcgcgcg acggaactct gccatcactt cacgcccgga gaaaccttgc
    15541 tccgcaccac acggatcttc tgcataagcc agcgaacctt tcaggtattt accaatttta
    15601 atcgcttcgt tcagcgacca ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag
    15661 cgttgcgcca gtgccacaat agactcggcc tcttcttccc cggccagtac accgcctttc
    15721 agtttgaaat cgttgaaacc atatttttca tacgccgctt ccgccaggcg caccaccgca
    15781 tccggcgtca tcgcttcttc atgacgcagg cgataccagt cgcatgagtc atccggctgg
    15841 ctttgatacg gcagcggtgt ggctttgcga ttaccgacga agaacagata accgagcatt
    15901 tcgacttcgc tacgctgttg accatcgccc agcagcgatg ccacgtttac ccccagatgc
    15961 tgccccagca gatccagcat tgccgcttct atcccggtaa ctacatgaat agtggtacgc
    16021 aaatcaaatg tctgcaaacc gcgcccgccg gcatcacgat cggcaaaagt attacgcacc
    16081 agcgtcagaa cgtttttgta ttcacccagc gttttaccca ctaccagcgg aatcgcatct
    16141 tccagcgttt tacggatttt ctcgccgccg ggaatttccc ctacgccagt gtgaccagaa
    16201 ttatctttga taatcacaat attacgcgtg aagaacggtg cgtgtgcacc actcagattc
    16261 atcagcatac tgtcatggcc cgccaccggg atgacctgca tttcagtaac aacaggcgtc
    16321 gtaaattgag aactcatatt tatgtccttt ttcagaatca atgacggccg aaaacgggac
    16381 gtttacggtc aaaggtccag ccggggatca ggtactgcat cgggcctgcg tcgttacgcg
    16441 caccgccagg cagacgttta taggcctcat gtgccttttg tacctgttcc cagtccagtt
    16501 ccacgcccag accgggcgca tcaggaacgg caatttttcc gtttttaatc tccagcggat
    16561 tttgggtcag gcgacaatcg ccctcctgcc aaatccagtg ggtatcgata gcggtaggat
    16621 tacccggcgc cgccgcgccc acatgggtaa acatcgccag agagatatcg aaatggttat
    16681 tagaatggca gccccaggtc agcccccagt cgtcgcaaag ctgcgccaca cggactgcac
    16741 cggaaagcgt ccagaagtgc ggatcggcaa gtggaatatc taccgcattg agcatcaccg
    16801 catgacccat ttcgcgccag ttggtggcga tcatgttagt cgcgacgggc aagccggtcg
    16861 cccgccgaaa ctccgccatc acttcacgtc cggagaagcc ctgttctgcg ccgcatggat
    16921 cttcggcata ggtaagaaca tcattcagcc ctttgcacag agaaatggct tcatcaagca
    16981 gccatgcacc gttgggatca acggtaatcc gcgcatccgg gaagcgtttc ttcaatgcac
    17041 gaacagtgtc gatttcttgc tcgccaggta acacgccgcc cttaagttta aaatctttaa
    17101 agccgtagcg atcctgtgag gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt
    17161 tctgatgccg caactgatac cactcatggt tgcccggcgt attttccaga taaggaagat
    17221 cggttttggt ccgatcaccg atataaaaca gataaccgag gacggtaata gcctcgcgtt
    17281 gcttgcctgg ccctaacagt tcgcagaccg gaacattcag cgccttacct aacaggtcaa
    17341 gcaaggcggc ttccagcgcc gctacggcat taacgcgcaa ttcaaaagtc caggcacctt
    17401 tgccgaaggt atcaaaatcg gctgcttgat tacctttatg cacctgctga accactttat
    17461 tcaggcgcgc gacttcctgg cccagcacca tcggaatagc ttcgacaagc gtctgataaa
    17521 tcacctctcc gcccggcgct tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca
    17581 caatattgcg ggtgaaatat gcgttatgtg ccccaccaat attaagcaac atgctatcgt
    17641 gcccggcgac cggaatgact ttcatatcag taataacagg actggattgt gtcgtcatct
    17701 tattgccccg cgacaggttt cagctcgata cgcttgatat cgcccaccag caccaggtag
    17761 ctcagtaccg cgattaaggc atgaacacca acataaatca gcgccccatt aaacgagcca
    17821 gtcgtgccaa cgatataacc aattgcgatt ggcgtgacga tgccagaaat gttaccgaac
    17881 atgttgaaca ggccaccgga aagaccgctg atctcttttg gcgcggtatc tgccattact
    17941 gcccaaccca gcgccccgat gcccttaccg aagaaggcca gcgccataaa gccgatgatc
    18001 atccactcaa cgttgacgta gttgcagaac accatcacca tcgacagcaa catgcccatt
    18061 acgatcggtg ttttacgcgc aatgttcagc gaccccgtgc ggcgcatcag ccagtcggaa
    18121 attatcccac ccagcacacc gccgataaaa ccgcaaaccg ccggaacgga agccacaaag
    18181 cccgctttca gaatcgacat cccgcgcgcc tgcaccagat aaaccgggaa ccaggtaatg
    18241 aagaagtaag tcagggcgtt gatacagtac tgaccgatat aaacgccgat catcatccgt
    18301 gatccgagca gctgtttgat ctgcccccac ttcacgctga acggcacttt aactttggtg
    18361 ttttgctgat ccatattgat cagcgcgcca cccgcggcga tgtactccag ctctttctga
    18421 tttacccccg gatgttgatt tggctcgtgg atgactttca accagataaa gctgatgaca
    18481 atccccagac cgcccataaa gaagaagacg tgtgaccagc ccacttcatg cgtcagccag
    18541 cccataatcg gcgcgaagat caccgttgcg aagtattgag cggagttaaa aatcgacacc
    18601 gccgttcccc tttcctgcgc cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa
    18661 gatggcgctt cagcaagccc gaccaggaag cgcagagtaa acagggcaac gataatgccg
    18721 aatccactaa agatatcgac gaagccttgc agcaaggtaa acatcgacca gataaagatc
    18781 gaccagaagt agacgcgttt tgaaccaaaa cgatccagca accaaccacc agggatctgc
    18841 ccgataacat aagcccatga gaaggcagag aacacatagc ccattcccac gggatcaagg
    18901 ccgatatctt tggccatttc cgaaccggca atagaaagcg tagcgcggtc gccgtagttg
    18961 aaggatgtga cgataaacaa catcaccact atccagtaac gagcattcgt gcgtttttcc
    19021 acacggctcg cagcctgact taaagaactc attgttgcac tcctgaaaat tcgcgttagc
    19081 cacgctcact ctggactgcg acatcgccag gaaatcagag gtgacgtagg gtgttttttg
    19141 ccgtttttat aggtcgttcg ccgaatacgg cgcgtgttta tatctggcaa tagcagtata
    19201 aaaagcgcgc catagcggct caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc
    19261 cgattttggc attagccctg gacggtggaa tccacttcac ggaaatgaaa acaagaacaa
    19321 gaaaggaagg gttaaaacga agaaataaag aagagtatga aatggatcgc ttgactccag
    19381 gcaaacgcca gtaaaaatcc gcgctatgaa gcagttttta ctggcatttg cctgaaaaga
    19441 ttcgattcag caccgctaaa acgacattta ccgctcgctg aacatatcag gacaacagcg
    19501 tgccccactg ttcgacccac ggatttgatt ccgtttccgg ttccgggttc tcactggcgt
    19561 caatcaacag catttcgcca acccgctgag cgctctgttc ctgcaacaag gcatcgaact
    19621 gtttgccgcc attgcagaaa tttacataac tgctgtcgcc gagcgcaatc acgccataac
    19681 gcagattcgg ctggaagccc agactatctt tgattccctg aaagagcggc acaatgctat
    19741 caggaaggtc gccctgcccg gtcgtggacg taaccaccag aacatactta tcctgatagg
    19801 gcagccagtc gcttaattca ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca
    19861 gaattgcttc tgcttcttcc gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa
    19921 taccaatttc cgccataccg ttttctccct ggattaggaa cttatctctc catcctgacc
    19981 tgatggcgca ctgaactcaa ccctttcatt ttcaggaagc agaccgcgcc agccaaattg
    20041 tgataacgcc tgcatccagg tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt
    20101 aaaaggatgc gtcaatgaca actgactggc atgcagcatt aaccgctgaa gaccaaaatg
    20161 ctcagcaccg ctgcgattct ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg
    20221 acgcaaatgg gcaagatgtc ggcgaagctg atgtttgcgt ccggttttcg gctccagttc
    20281 caccaggccg tagcgcgtgg tcgggtaacg tccggtcgct accggcattt ctacggtcgc
    20341 cagaccgcga taatgcgtca ctgctggctg cgggccttta tcttcgcggg caaatttatc
    20401 agcgattttg tccagttctt ccaccagtgg ataatccagc accgcttctt ccatcaacca
    20461 gccgcgcaca atcgcatggt aacgtttctg gatttggtgc tgttcaaact gttgtgccag
    20521 cagccgtccg gcctcgctgg ataatcccat caacaacaca ccagaagtgg gtcggtccag
    20581 acgatgagca gtaaaaacat gctgtcctat ctggtcacgc acggtttgca tgaccactac
    20641 tttctcgtcg cgatccagcc agctgcggtg aaccagccag ccggagggtt tatttaccgc
    20701 aaccagccat tcatcctgat agagtatttc cagcattagc tcgcatcatc cgcaaaaaga
    20761 gcatccagtt tttccaactc agccagaata agcgcgcgtt gcggatggtc cgtcgccagc
    20821 gccatttcat aatagggagc aacggcaaaa gcgcccggta acggctggtt gttatttaac
    20881 aaatcgtgca ttcgcgggat cagcacccac tgcaaccact ccagtggttc catggtgtcc
    20941 ataaagaacg gttgggtact attaaattga tgcggctggg gtacatcgtt tcgccagtgc
    21001 tgatgttcac gcagtaacgc ttcaagcgcc tgcaactgga gacgaacgcg gtcatgagtg
    21061 gtcataaagc aacctcaata agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg
    21121 agcactgtat tcacagtgct cccggttcgt ttcgcagcat tccagctaca tttgttgctc
    21181 cctgctcatc cttgacaact tttcctctgg ccttgcggcc aatcgttcat cctgaactat
    21241 tgcttcctgc tcacaccacc ccgatgtgat acttcatcct gaagtgtccc tggccttcct
    21301 gacccaccga atcatcctga ccggttctca ttctccatcc tggaggtgtc ctttaacgcg
    21361 tcctgcgtca tcctcttcgc ttcatccaga agcctttccc tgaaacacca tcctggtgtg
    21421 tccttcagaa gtgtcatcat cctgatgttc acttcgttgt ctgactccct gtcgacgaag
    21481 atagaatcgt ctttttcgcg aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa
    21541 tttcatatga aagttaggaa ttaacaagca acatgttgtt tttaaatgaa aataacgatt
    21601 cttatctttt taagtattcg cttatttcct ggcgatctcc tacagagtct gtaagagatc
    21661 tctcacaacc agatggggta tttggattac agaagaggct taagttgatt aaggaattcc
    21721 gcaagatttg aggctaaatg cgtccgttta cgtgttccca gcgtctcttt acacacttca
    21781 ccggagagat tacacaccga gatgacctct aactcttctt ccagcgtcgc gataaatagc
    21841 gtgggtggca gttttagcct tttctgagtc accagatgac caatcagatt ttcctgcacc
    21901 cgacggaagt cgtcttcact ccaggtttgc agcaatgtca gtttgatatc gccaaactgc
    21961 gcgtgcatat ccccggcaaa ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca
    22021 atatcaaaag cgcgttcaac cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc
    22081 cagtatacgg catcttcagt agttgaaata atgcacggtg aaggaacgcc gtacagttcc
    22141 tcacttaacg gccagctttt atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa
    22201 tctttcaggg cttgtgcggt caaatcgtcc actaatttct ctcttcactt aaaccagata
    22261 cacttgtcgt ttagtttatc tggtttatga cggtgaaaca tgtcttctta tgcaaaccat
    22321 caggcacttg cgggcctgac tcttggaaaa tcaaccgatt accgggatac ctatgacgcc
    22381 agcctactgc aaggcgttcc acgcagcctg aatcgcgatc cgctgggtct gaaagcggat
    22441 aacctgcctt ttcaaggtgc ggacatctgg acgctgtatg aactttcctg gctgaatgcg
    22501 aaaggtttgc cgcaggtcgc tgtcggtcat gttgaacttg attacaccag cgtaaatctg
    22561 attgagtcga agagttttaa gctctatctc aacagtttta accagacgcg ttttaataac
    22621 tgggatgagg tgcgccagac gctggagcgc gacttaagca cttgcgctca gggtaatgtt
    22681 agcgtggcgt tatatcgtct tgatgaactg gaaggccagc cgataggtca tttcaatggc
    22741 acttgcattg atgaccagga tatcactatc gataactatg aattcactac tgactatctg
    22801 gagaatgcca cctgtggtga aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa
    22861 tcaaactgcc tgatcaccca tcaaccagat tggggttcga tccaaattca gtatcgtgga
    22921 cgccaaattg acagagaaaa actgctgcgt tacctggtct cattccgtca tcacaacgag
    22981 ttccacgaac agtgcgtgga acgcatcttt aatgacctgt tacgcttctg ccagccagag
    23041 aaattgagcg tttacgcacg ttatacccgt cgtggcggtc tggacattaa cccgtggcgc
    23101 agtaatagcg attttgtccc atcgaccaca agactggttc ggcaataaat tttttctcaa
    23161 ttttgcgtgc tggattcacg cagaaggttg tgaaaggtca tcaggcaggg ctattgtaat
    23221 caaagggaat gacgatattc gtcccataag gagtttttct tgattacaca tattagcccg
    23281 cttggctcca tggatatgtt gtcgcagctg gaagtggata tgcttaaaca caccgccagc
    23341 agcgacctct atcaattgtt tcgcaactgt tcacttgccg tactgaactc cggtagtttg
    23401 accgataaca gcaaagaatt gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc
    23461 cgtgaacgcg gcgtaaagct ggaactgatt aatcccccgg aagaggcttt tgtcgatggg
    23521 cgaattattc gcgctttgca ggccaacttg ttcgcggttc tgcgagacat tctcttcgtt
    23581 tacgggcaaa tccataacac cgttcgtttt cccaacctga atctcgacaa ctccgtccac
    23641 atcactaacc tggtcttttc catcttgcgt aacgctcgcg cgctgcatgt gggtgaagcg
    23701 ccaaatatgg tggtctgctg gggcggtcac tcaattaacg aaaatgagta tttgtatgcc
    23761 cgtcgcgtcg gaaaccagct gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg
    23821 ggagcgatgg aagcgccgat gaaaggtgct gcggtcggac acgcgcagca gcgttacaaa
    23881 gacagtcgtt ttattggtat gacagagccg tcgattatcg ccgctgaacc gcctaacccg
    23941 ctggtcaacg aattgatcat catgccggat atcgaaaaac gtctggaagc gtttgtccgt
    24001 atcgctcacg gtatcattat cttccctggc ggtgtgggta cggcagaaga gttgctgtat
    24061 ttgctgggaa ttttaatgaa cccggccaac aaagatcagg ttttaccatt gatcctcacc
    24121 ggcccgaaag agagcgccga ctacttccgc gtactggacg agtttgtcgt acatacgctg
    24181 ggcgaaaacg cgcgccgcca ttaccgcatc atcattgatg acgccgctga agtcgctcgt
    24241 cagatgaaaa aatcgatgcc gctggtgaaa gaaaatcgcc gcgacaccgg tgacgcttac
    24301 agttttaact ggtcaatgcg cattgcgcca gatttgcaaa tgccgtttga gccgtctcac
    24361 gagaatatgg ctaatctgaa gctttacccg gatcaacctg ttgaagtgct ggctgccgac
    24421 ctgcgccgtg cgttctccgg tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc
    24481 attgaagagt ttggtcctta caaaatcaac ggcgataaag agattatgcg tcgtatggac
    24541 gacctgctac agggttttgt tgcccagcat cgtatgaagt tgccaggctc agcctacatc
    24601 ccttgctacg aaatctgcac gtaatctccc ctcccccggt gacgtttgtt gccgggtttt
    24661 ctcgtttttg gacacttact catcaactca tttcatttgt tatatgaatg tttcttacca
    24721 ccctcacgcg a
//
LOCUS       DAZKFU020000047        24154 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZKFU020000047 DAZKFU020000000
VERSION     DAZKFU020000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.047"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..419
                     /gene="cmtB"
                     /locus_tag="ACHOQQ_RS07560"
                     /old_locus_tag="pgaptmp_001512"
     CDS             <1..419
                     /gene="cmtB"
                     /locus_tag="ACHOQQ_RS07560"
                     /old_locus_tag="pgaptmp_001512"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGU4469781.1"
                     /translation="SSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINN
                     GPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIGLSATDADSH
                     IGAIQALSELLCEEEILEQLLTASSEKKLADIISRG"
     gene            447..1835
                     /gene="cmtA"
                     /locus_tag="ACHOQQ_RS07565"
                     /old_locus_tag="pgaptmp_001513"
     CDS             447..1835
                     /gene="cmtA"
                     /locus_tag="ACHOQQ_RS07565"
                     /old_locus_tag="pgaptmp_001513"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGU4469782.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1850..3127
                     /gene="yggP"
                     /locus_tag="ACHOQQ_RS07570"
                     /old_locus_tag="pgaptmp_001514"
     CDS             1850..3127
                     /gene="yggP"
                     /locus_tag="ACHOQQ_RS07570"
                     /old_locus_tag="pgaptmp_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4469783.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3124..4089
                     /gene="glpX"
                     /locus_tag="ACHOQQ_RS07575"
                     /old_locus_tag="pgaptmp_001515"
     CDS             3124..4089
                     /gene="glpX"
                     /locus_tag="ACHOQQ_RS07575"
                     /old_locus_tag="pgaptmp_001515"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4469784.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVEVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4111..4620
                     /gene="fumE"
                     /locus_tag="ACHOQQ_RS07580"
                     /old_locus_tag="pgaptmp_001516"
     CDS             4111..4620
                     /gene="fumE"
                     /locus_tag="ACHOQQ_RS07580"
                     /old_locus_tag="pgaptmp_001516"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGU4469785.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMNTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4617..5330
                     /locus_tag="ACHOQQ_RS07585"
                     /old_locus_tag="pgaptmp_001517"
     CDS             4617..5330
                     /locus_tag="ACHOQQ_RS07585"
                     /old_locus_tag="pgaptmp_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGU4469786.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIKRVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5302..5979)
                     /locus_tag="ACHOQQ_RS07590"
                     /old_locus_tag="pgaptmp_001518"
     CDS             complement(5302..5979)
                     /locus_tag="ACHOQQ_RS07590"
                     /old_locus_tag="pgaptmp_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4469787.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     QPDDIHP"
     gene            complement(5973..6650)
                     /locus_tag="ACHOQQ_RS07595"
                     /old_locus_tag="pgaptmp_001519"
     CDS             complement(5973..6650)
                     /locus_tag="ACHOQQ_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4469788.1"
                     /translation="MVTLEQFRYCPTHTTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPHASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6638..7345)
                     /locus_tag="ACHOQQ_RS07600"
                     /old_locus_tag="pgaptmp_001520"
     CDS             complement(6638..7345)
                     /locus_tag="ACHOQQ_RS07600"
                     /old_locus_tag="pgaptmp_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGU4469789.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLH"
     gene            complement(7346..7924)
                     /locus_tag="ACHOQQ_RS07605"
                     /old_locus_tag="pgaptmp_001521"
     CDS             complement(7346..7924)
                     /locus_tag="ACHOQQ_RS07605"
                     /old_locus_tag="pgaptmp_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGU4469790.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPICTTRHFPAMAAVR"
     gene            complement(7947..8378)
                     /locus_tag="ACHOQQ_RS07610"
                     /old_locus_tag="pgaptmp_001522"
     CDS             complement(7947..8378)
                     /locus_tag="ACHOQQ_RS07610"
                     /old_locus_tag="pgaptmp_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HGU4469791.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8750..9769
                     /gene="epd"
                     /locus_tag="ACHOQQ_RS07615"
                     /old_locus_tag="pgaptmp_001523"
     CDS             8750..9769
                     /gene="epd"
                     /locus_tag="ACHOQQ_RS07615"
                     /old_locus_tag="pgaptmp_001523"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGU4469792.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRSEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9819..10982
                     /gene="pgk"
                     /locus_tag="ACHOQQ_RS07620"
                     /old_locus_tag="pgaptmp_001524"
     CDS             9819..10982
                     /gene="pgk"
                     /locus_tag="ACHOQQ_RS07620"
                     /old_locus_tag="pgaptmp_001524"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGU4469793.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11197..12276
                     /gene="fbaA"
                     /locus_tag="ACHOQQ_RS07625"
                     /old_locus_tag="pgaptmp_001525"
     CDS             11197..12276
                     /gene="fbaA"
                     /locus_tag="ACHOQQ_RS07625"
                     /old_locus_tag="pgaptmp_001525"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGU4469794.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12340..12438)
                     /locus_tag="ACHOQQ_RS07630"
                     /old_locus_tag="pgaptmp_001526"
                     /pseudo
     CDS             complement(12340..12438)
                     /locus_tag="ACHOQQ_RS07630"
                     /old_locus_tag="pgaptmp_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12634..13494
                     /gene="mscS"
                     /locus_tag="ACHOQQ_RS07635"
                     /old_locus_tag="pgaptmp_001527"
     CDS             12634..13494
                     /gene="mscS"
                     /locus_tag="ACHOQQ_RS07635"
                     /old_locus_tag="pgaptmp_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU4469795.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13633..14268
                     /gene="argO"
                     /locus_tag="ACHOQQ_RS07640"
                     /old_locus_tag="pgaptmp_001528"
     CDS             13633..14268
                     /gene="argO"
                     /locus_tag="ACHOQQ_RS07640"
                     /old_locus_tag="pgaptmp_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGU4469796.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14361..15101
                     /gene="yggE"
                     /locus_tag="ACHOQQ_RS07645"
                     /old_locus_tag="pgaptmp_001529"
     CDS             14361..15101
                     /gene="yggE"
                     /locus_tag="ACHOQQ_RS07645"
                     /old_locus_tag="pgaptmp_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGU4469797.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15269..16165
                     /gene="ygfI"
                     /locus_tag="ACHOQQ_RS07650"
                     /old_locus_tag="pgaptmp_001530"
     CDS             15269..16165
                     /gene="ygfI"
                     /locus_tag="ACHOQQ_RS07650"
                     /old_locus_tag="pgaptmp_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4469798.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16162..17640)
                     /gene="scpC"
                     /locus_tag="ACHOQQ_RS07655"
                     /old_locus_tag="pgaptmp_001531"
     CDS             complement(16162..17640)
                     /gene="scpC"
                     /locus_tag="ACHOQQ_RS07655"
                     /old_locus_tag="pgaptmp_001531"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGU4469799.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17664..18449)
                     /gene="scpB"
                     /locus_tag="ACHOQQ_RS07660"
                     /old_locus_tag="pgaptmp_001532"
     CDS             complement(17664..18449)
                     /gene="scpB"
                     /locus_tag="ACHOQQ_RS07660"
                     /old_locus_tag="pgaptmp_001532"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGU4469800.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18460..19455)
                     /gene="meaB"
                     /locus_tag="ACHOQQ_RS07665"
                     /old_locus_tag="pgaptmp_001533"
     CDS             complement(18460..19455)
                     /gene="meaB"
                     /locus_tag="ACHOQQ_RS07665"
                     /old_locus_tag="pgaptmp_001533"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGU4469801.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIRDGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAETAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19448..21592)
                     /gene="scpA"
                     /locus_tag="ACHOQQ_RS07670"
                     /old_locus_tag="pgaptmp_001534"
     CDS             complement(19448..21592)
                     /gene="scpA"
                     /locus_tag="ACHOQQ_RS07670"
                     /old_locus_tag="pgaptmp_001534"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HGU4469802.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARVIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21796..22689)
                     /gene="argP"
                     /locus_tag="ACHOQQ_RS07675"
                     /old_locus_tag="pgaptmp_001535"
     CDS             complement(21796..22689)
                     /gene="argP"
                     /locus_tag="ACHOQQ_RS07675"
                     /old_locus_tag="pgaptmp_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGU4469803.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            complement(22760..24085)
                     /locus_tag="ACHOQQ_RS07680"
                     /old_locus_tag="pgaptmp_001536"
     CDS             complement(22760..24085)
                     /locus_tag="ACHOQQ_RS07680"
                     /old_locus_tag="pgaptmp_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HGU4469804.1"
                     /translation="MKKANLTGTLVCFVACIIFLSSSDIFREKPIALIWFIRQNTLFF
                     TGTLAWCLMTLTMCLSLRSSWLNKVLGGLDKAWRLHKWAGICAIAFAFAHWLDEKLPQ
                     LFVAFGWLTHPGKIVDINLTPVQENWLHAGLLAGECAMFIMIAMVFVSLSKKVPYHLF
                     HLVHRLFPVFYLAIAFHVFTALFKSYWWETPAAYLLILITIPGVFVAFISLLKLNGNK
                     SKHQATVKSIVNHPGQITEVILEMEHPIDYCPGQFAFLTFAHSKESHPFTIASYVKEK
                     NTLRFAIKNLGDYTSTLASSIQIGQSALVEGPWGKFDFTLPCSRQVWIAGGIGITPFI
                     AQLEYLNQHGASRVPVDLWYCVSRSEDAWYVDKLTSLCAQASVTLHLLDAHKGERLQS
                     HYLTDKIANKGDTHFWFCGPQSFAKALSKGLYENGIASQNFHYDRFCMR"
BASE COUNT         5904 a         5945 c         6407 g         5898 t
ORIGIN      
        1 aatcatcaat ctcagttata cattcagcaa aagattggca ggaagctatc gatttctcga
       61 tggtatcatt gctggataaa aactatatca gcgagaatta cattcaggca attaaagatt
      121 ccaccattaa caatggccct tattatattc tcgcaccagg cgtggcaatg cctcatgcgc
      181 gaccggaatg tggggcgctt aaaaccggga tgtctttgac attacttgaa caaggtgttt
      241 attttccggg gaatgatgaa ccaattaaat tactcatcgg actctctgct accgatgccg
      301 attcgcacat tggcgctatt caggcgttaa gtgaattact gtgcgaagaa gaaatactcg
      361 aacaactctt aacagcatca tcagaaaaaa aattagcgga cattatcagc cgcggataat
      421 tactctctcc tctacaggat aatattatgg aaaacaagtc tgctcgtgca aaggtccagg
      481 cttttggggg ctttttgact gcaatggtca tccccaatat tggtgctttt attgcctggg
      541 gttttattac tgcgttattt attcccaccg gttggctgcc taacgaacat ttcgccaaaa
      601 ttgtcggccc gatgattacc tatttattgc ccgtgatgat tggttctacc ggtggtcatc
      661 tggtcggcgg taaacgcggc gcggtcatgg gcggaatagg tactattggt gtgatcgtcg
      721 gcgcagagat cccgatgttc cttggctcaa tgattatggg gccgctcggt ggtctggtca
      781 taaaatatgt tgataaggca ctggaaaaac gcatacctgc cggttttgag atggttatca
      841 ataacttctc attaggtatc gcggggatgc tcctttgcct gctgggtttt gaagtgatcg
      901 gcccggcggt gttaattgcc aatactttcg tcaaagagtg tattgaggcg ctggtacatg
      961 cgggttatct gcctctgctg tcagtcatca atgaaccggc gaaagtgctt ttccttaata
     1021 atgcgatcga tcagggcgtc tattatccgc tgggaatgca acaggcttcg gttaacggta
     1081 aatccatctt ctttatggtg gcctctaacc caggtccggg cctggggctg ctgctggcgt
     1141 ttaccttgtt tggtaaaggg atgagtaaac gttctgcgcc cggggcgatg attattcact
     1201 tcctcggtgg gatccacgaa ctgtatttcc catatgtgct gatgaagccg ctgactatta
     1261 ttgccatgat tgcgggcggt atgtctggca cctggatgtt taacttactg gacggtggtc
     1321 tggtggctgg cccaagtccg gggtctatct ttgcttacct ggcgctgacg ccgaaaggtt
     1381 cgttcctggc gacaattgcc ggtgttacgg taggtaccct ggtgtccttt gctatcactt
     1441 cgctgatact gaagatggaa aaaacggtgg aaacggagag cgaagatgag tttgctcagt
     1501 cagccaatgc ggttaaggcg atgaaacaag agggtgcatt ctcgttaagc agggttaagc
     1561 gtatcgcctt tgtttgcgat gcggggatgg gctccagtgc gatgggcgcg accaccttcc
     1621 gtaaacgcct ggaaaaagcg gggctggcaa ttgaagtaaa acattacgcc atagaaaacg
     1681 tgcctgcgga tgcggatatc gtcgttactc atgccagtct ggaagggcgc gtgaaacgtg
     1741 tgacggataa accactgata ttgattaata actatattgg cgatccaaaa ctcgacactt
     1801 tatttaatca attaaccgcc gaacataaac actgattaca gaggtaaaaa tgaaaaccaa
     1861 agttgctgct atttatggca agcgggatgt ccgtctgcgc gaatttgaac tgccagaaat
     1921 taccgataat gaattactgg tgagtgtgat ttctgacagc gtctgtttat ccacctggaa
     1981 agcggcgtta ctcggtagtg aacataaacg cgtacccgac gatttagaaa atcatccggt
     2041 cattaccggg catgaatgtg ccggggttat tgtcgaagtg ggtaaaaatc tcactggaaa
     2101 atataaaaaa ggccagcgtt ttgtattgca accggcgatg gggttaccaa gcggatattc
     2161 agcgggctac agctacgaat attttggcgg caatgccact tatatgatta ttcccgaaat
     2221 cgccattaat ttgggctgcg tattaccgta tcacggctct tattttgctg cggcgtcgct
     2281 ggcagagcct atgtgctgca ttattggtgc ttatcatgcc aattatcaca ccacgcaata
     2341 tgtttatgag catcgcatgg gcgtcaaacc tggcggcaat attgcactgc tggcgtgtgc
     2401 aggtccgatg ggcattggcg ctatcgatta cgccattaac ggcggcatac aaccgtcgcg
     2461 ggtggtggtg gtcgatatcg acgacaaacg tctggcgcag gtacagaagc tgctgccggt
     2521 ggaactggcg gccagtaaag gcattgagct ggtgtatgtg aataccaaag ggatgagcga
     2581 tcctgtccag atgctgcggg cgctgacagg agatgccggg ttcgatgaca tttttgttta
     2641 tgcggcggtg cctgctgtcg ttgagatggc tgatgaatta ctggcggaag atggctgtct
     2701 gaacttcttt gccgggccga cggataaaaa cttcaaagtg ccgtttaatt tctacaacgt
     2761 tcattacaac agcacgcacg tcgtcggtac atccggcggt tcaacggacg acatgaaaga
     2821 ggcgattgcc cttagcgcca ctgggcagtt acagccgtcg tttatggtga cccatatcgg
     2881 tggcctggat gcggtgccag aaaccgtgct caatctgccg gatatccctg gcggtaaaaa
     2941 actcatttat aacggcgtga ccatgccgct cactgccatt gccgattttg ccgaaaaagg
     3001 caaaaccgat ccgctgttta aagagttggc gcggctggtt gaggaaacgc acggcatctg
     3061 gaatgaacag gccgagaaat atctgctggc acaatttggc gttgatattg gggaggccgc
     3121 gcaatgatgt ccctggcgtg gccattattt cgcgttacgg aacaggcagc gttggctgcc
     3181 tggccgcaaa ccggatgtgg cgacaaaaat aaaattgatg gcctggcggt caccgcgatg
     3241 cgccaggcat taaacgacgt cgcttttcgt ggacgagtgg ttatcggcga aggagaaatt
     3301 gaccatgcgc cgatgctgtg gattggcgaa gaggtgggca aaggcgatgg gccagaagtc
     3361 gatatcgcgg ttgatcccat tgaaggcacg cggatggtgg cgatggggca gagcaatgcg
     3421 ctggcggtca tggctttcgc ccctcgagat agcctgttac atgcgcccga tatgtatatg
     3481 aaaaagctgg ttgttaatcg actggctgcc ggggcgattg atctgtcttt gcctctggcg
     3541 gacaacttgc gtaacgtggc gcgggcgtta ggtaaaccgc tggacaagct gcgcatggtt
     3601 acgctggata aaccgcgtct gagtgccgcg attgaggaag caacacagct gggggtgaaa
     3661 gtttttgccc tgccggatgg cgatgtcgcc gccagcgtgc tgacctgctg gcaggataat
     3721 ccttacgatg tgatgtacac catcggcggt gcgccggaag gcgtgatttc tgcctgtgca
     3781 gtcaaagcgt taggcggaga tatgcaggcg gaattaatcg atttttgtca ggctaaaggg
     3841 gattacacgg aaaatcggca gattgcagag caggagcgca agcgttgcaa ggcaatgggc
     3901 gttgaagtga accgtgtgta ctcgctcgac gaactggtga gggggaacga tatcctcttt
     3961 agcgctacgg gcgtcacggg cggcgagctg gtgaacggta tccaacagac ggcgaatggg
     4021 gtgcggacgc agacattact gatcggcggc gcggaccaaa cgtgtaatat aatagactcc
     4081 ctgcattgat ggtgatctat cggaagacca atggcgacgc tgacagaaga tgatgtgctt
     4141 gagcaactgg atgcacagga caatttattt tcatttatga atactgcgca ttctattttg
     4201 ctccagggga tacgccagtt tctgccgtcg ctgtttgtcg ataacgatga agagatcgtc
     4261 gaatatgcag tgaagccgtt actcgcccaa agcggcccgc ttgacgatat tgatgttgca
     4321 ctgcgtttga tttatgcgct ggggaaaatg gataaatggc tgtacgccga tatcacgcat
     4381 ttttcccagt actggcatta cctgaaagaa caggatgaaa cgctcggatt tgccgatgac
     4441 atgacttggg attttatcag caacgtcaat agcataacct gcaatgcgac gctctatgac
     4501 gcgttgaaag cgatgaagtt cgccgatttc gccgtctggt cagaggcgcg ttttagcggg
     4561 atggtcaaaa cggcgctgac gctggcagta acgaccacct taaaggaatt aacgccgtga
     4621 aaattgaatt aacggtgaat gggctgaacg tgcaagcgca gtaccatgat gaagagataa
     4681 aacgcgtgca taaaccgttg ctgcgtatgc tggcagcctt acagacggta aatccgcagc
     4741 ggcgtactgt ggtttttctt tgcgctccgc cggggacggg gaaatctacc ctaaccacct
     4801 tctgggaata tctcgcgcaa caagacccgg aactgcccgc tattcagacg ctgccgatgg
     4861 atggttttca ccattacaat agctggctgg atgcgcatca attgcgcccc ttcaaaggcg
     4921 caccagagac atttgacgta gcgaaactgg cggaaaatct gcgccaggtc gtggaagggg
     4981 attgtacgtg gccgcagtat gatcgacaaa agcatgatcc tgttgaagat gcgttgcacg
     5041 ttaccgcacc actcgtcatc gtcgaaggaa actggttgtt gctggacgac gagaagtggt
     5101 gccagcttgc gcaattttgt gatttctcga tctttatcaa tgcgccagct actgcgttgc
     5161 gggaacgact ggtggggcgc aagctggcgg gagggttatc gctggcagac gcagaagctt
     5221 tttatgaccg taccgacggg ccgaatgttc gacgggtgct ggaagagagt ctgcctgcaa
     5281 atttgacctt aatgatgacc gctacgggtg aatatcgtct ggttgattaa cgttcctgat
     5341 tagaccatct tccagtcgta ccacacgaga gaaatgacgc cgggtaaatg cggcgtcatg
     5401 actgattgct acgacgctcg tgcctcgctg gcggcacttc tccagccagc tttcaaacac
     5461 gctcaaccaa ttttcgtcaa aatcccgact cggctcgtcc agcagcaata gtggcggaga
     5521 gagtgcttcc aggcaggcga cggcgaccat ccgtcgctgc gcactatgca gatccaatgg
     5581 gtgcgcgctg gcagtgtctg ctaactgaca acattgcagg gcagcatggg tacgttgagt
     5641 gatttcatcc gccgggcatt tttgcagttt caaaccaaat gcgatctcgt cagcgacggt
     5701 gctgtgaaaa agttggtttt ccgcttcctg aaacagtacg ccaatctttg ccgcgcgctg
     5761 gcggtttttc aggtttttca tcgcctgttg ttgcagcata accgtaccgg ctgtgggcgt
     5821 aagaagacct gccattactc gtaacagcgt cgatttccct gcgccattgt caccggtcag
     5881 cgccaaccat tcaccttgtt tgagttgcag agaaatgtcg caaagacaat ccgttgccgc
     5941 gccaggccag cgataagata tctgatttaa cgttaacata gtggctggag cgctccgttt
     6001 tgcaattgcc acgcttgctg gcagtagttc aggaaaggtg tgcgatgacg ttcaaaaaga
     6061 ataatcagcg aaccacgttc aaacgcccag tgttgtaagc gctgcaacag catttcgctg
     6121 gcatgcggcg tcaggcggct aaaggcttca tcaagaatta atagctttgg ctgcatcgcg
     6181 atggcgcagg cgatgaccac gcgctgtgtc tctccaccgg agagtgttgc tgggtgacga
     6241 tggcgcaacg gctggcactc ggtcagagtc agggcggcat caatccgcgc cataatctct
     6301 gcttcagcaa gacacagatt ttctggccca aaggcaactt cttcttccac gctaaaggta
     6361 cagccggaga gctgcaaata gggcgattgc tggacgagct ggatggtggc ggattgttca
     6421 ttaagcggga gatgcccaat gggcgtaccg aggagcgtcc ccgtgcctgt tatctctcct
     6481 ggcagaaagt ccggatacca gcccgccatc agctgtgcca gcgtactttt gccactgccg
     6541 ttgtcaccaa agatggcgac catccccggt ttgacatagt gaaagtcata acagaacgga
     6601 ggatgcgttg tgtgtgtcgg gcaataacgg aactgttcta atgtaaccat atccacgctc
     6661 cgaattctgt cagcatcagc aagatcatgg tgtaacgcgc tacgcgttgt agggtgctat
     6721 cggctggtgc ccacaacgtt gttcgtcggt tgtggatccg aaatgcccgc atatccagcg
     6781 ccgcaccacg gacagcaaga tcgtttagcg cattgtgtgt tagcggaata attaacgcgg
     6841 gcatcgcacg taaacgctga taccagccct catccagcgg cacgccgcgg gcacgctgcg
     6901 cttcatgaat aatcgctaat tgccgtttta actgctcaac gaccaataac ggcccggcaa
     6961 acagataagc gacgcctggc ggcaggcgag aagcaaacag agcgcggata aaacattgca
     7021 cggggacgta ctgcatccac aactgtgagg ttgaaacaat cgccagaatc cgcaaccaga
     7081 gcgtaatagc atgcgtccaa cgctccggcg aacgcggcgt gccgcttagc cattctgtta
     7141 gccagccacc gtgtaccagc cacagcccgg ctcccagcga gaacatcagc cagacgacat
     7201 attttgcccg ccgccgcgtg gcctttagcg caataaggca aaaaaaggtc gccgcgctat
     7261 agatcggcaa aatggtctgc gcgggcagga taagcgtggt gcaggccgcc agcgcccaca
     7321 gcgttaagga tgtaaacgga tgcatttagc gcacggcagc cattgccgga aaatggcgcg
     7381 tggtgcaaat cggcagctgg cgcagtaaaa gccagacaat caccgctgta agaattttat
     7441 cgaccaggtt tgcgcctatc accgtaatcg ccaccgactc caccaggttt tgccccatag
     7501 agtgcatcca ggcaacgaac aaatcggctc cgctaccggt gacaccgccg aacagcgcag
     7561 tacgcagcgg tacagcgacg accgttaccg caagcgtaat aatcacgccg ctgacaacga
     7621 cttttggcag ggtgcggaac caacccgctc gcgccagcca accggcgacc aggccgatga
     7681 ccatcgccac gggtgcaaat gcggcggcga tcgggtcggt cagcaatccc cagagtaaat
     7741 tggtcagtaa gccggtcagc attccgatca ccggaccaag cagtaccgcg ctaataagtg
     7801 tgccgatgga gtcgagaaat ataggtaatt tgaccatgct ggccagttgc ccgccgatca
     7861 tattgatggc gatagatata acgatcaaca ccagtgcctg actggaaaag tggctacgcg
     7921 ccataagaaa acctctctga aagtagttat ttaacggggg aaatatgcaa ctcgtcgtaa
     7981 ccagaaagcg cgcaaggttg gcggctaaac gccagttcct caaggctacc gatcaccagt
     8041 tcaagcgtgg tccgcacggt acagccgggc atcatatgac cgccaagcat cgtgccgtgt
     8101 ggatcggaaa cgcagagatg aagatgctca ccactctgtt ccaacgtgcc gtttagtgag
     8161 atcacttcga atgtaccgtt caggagcgtg gtgccttctt gcccggcata gcgcaaggcg
     8221 atgtcagtca ggctaccggt acagcctgca atccaggcgg catgaagttg ctgctgctgt
     8281 gcaaaagcgt gtagctggga aaaaacttcc tgcccgggga ggaggcgcag tgcgtaaaag
     8341 cgtgcggagg aagaatatgg gtagggcaat gtcatcattg gagtactccg aaactcggac
     8401 gtattgcgga taatatcgac gctgcatcag tcatattctg atataagtca gggtttgtga
     8461 cggcacaacg aaaagatttt gctacgctta ttcgtgattc tgcaaagtgg aataaattcc
     8521 acaggggcgg ggctgaaaac gtgacgtaag tcagataatt gttttctttg actggacaat
     8581 cattcctttt attccacgtt tcgcttatcc tagctgaagc gtttcagtcg attaaatgtt
     8641 cgacaattaa ccaatcagtc gcagtttgcg acaggtaagg tttccccgga cgatttgctg
     8701 gattactctg tctggcaaat ttgctcaggg aaaaccttgc aggagatcta tgaccgtacg
     8761 cgtagcgata aatggcttcg gtcgcatcgg gcgtaatgtg gttcgtgctt tgtatgaatc
     8821 cggacgccgg gcggaaatta ccgtggtggc aatcaacgaa ctggcggatg ctgcgggcat
     8881 ggcgcatttg ttgaaatacg acaccagcca tggccgtttt gcatgggaag tacgacagga
     8941 acgcgatcaa ctttttgttg gtgatgacgc catccgcgta ttgcatgaac gttcactgca
     9001 atcgctcccc tggcgtgaac ttggcgttga tgtagtcctc gactgcaccg gcgtatatgg
     9061 ctcccgcgag catggcgaag cgcacattgc cgccggggct aaaaaagtgc tcttttcaca
     9121 tcctggcagt aacgatctcg acgcgaccgt tgtttacggc gtcaatcagg atcaacttcg
     9181 ttcggaacac cgcatcgttt ctaacgcttc ctgtaccacg aattgcataa ttcccgtcat
     9241 caaattgtta gatgatgcgt acggtattga gtccggcact gtgaccacaa ttcactccgc
     9301 catgcacgat caacaggtta ttgatgcata ccatcctgac ctgcgtcgca cccgggcagc
     9361 cagccagtcg atcattccgg tcgatactaa actggccgcc ggtatcacac gattttttcc
     9421 gcaatttaac gatcgctttg aagcgattgc ggtacgtgtg ccaaccataa atgtgacggc
     9481 aatcgattta agcgtgacgg tgaagaaacc tgtaaaagcc aatgaagtca acctgttgct
     9541 gcaaaaagca gcacaaggtg catttcatgg tatagttgac tatacggaat tgccgttggt
     9601 ctctgtagat tttaaccacg atccgcacag tgccattgtc gatggcaccc aaacccgggt
     9661 cagtggcgca cacctgatca aaacgttggt ctggtgcgat aacgaatggg gctttgctaa
     9721 ccgaatgctc gacacgacgt tagctatggc tactgttgct ttcaggtaag acgcaagcag
     9781 cgtctgcaaa acttttagaa tcaacgagag gattcaccat gtctgtaatt aagatgaccg
     9841 atctggatct tgctgggaaa cgtgtattta tccgtgcgga tctgaacgta ccagtaaaag
     9901 acgggaaagt aaccagcgac gcgcgtatcc gtgcttctct gccgaccatc gaactggccc
     9961 tgaaacaagg cgcaaaagtg atggtaactt cccacctggg tcgtcctacc gaaggcgagt
    10021 acaacgaaga attctctctt ctgccggttg ttaactacct gaaagacaaa ctgtctaacc
    10081 cggttcgtct ggttaaagat tacctcgacg gcgttgacgt tgctgaaggt gaactggttg
    10141 ttctggaaaa cgttcgcttc aacaaaggcg agaagaaaga cgacgaaacc ctgtccaaaa
    10201 aatacgctgc actgtgtgac gtgttcgtaa tggacgcatt cggtactgct caccgcgcgc
    10261 aggcttctac tcacggtatc ggtaaattcg ctgacgttgc gtgcgcaggc ccgctgctgg
    10321 cagctgaact cgacgcgctg ggtaaagcac tgaaagaacc tgctcgcccg atggtggcta
    10381 tcgttggtgg ttctaaagta tctaccaaac tgaccgttct ggactccctg tctaaaatcg
    10441 ctgaccagct gattgttggt ggtggtatcg ctaacacctt tatcgcggca caaggccacg
    10501 atgtgggtaa atccctgtac gaagctgacc tggttgacga agcaaaacgt ctgctgacca
    10561 cctgcaacat cccggttccg tctgatgttc gcgtagcaac cgagttctct gaaaccgctc
    10621 cggctaccct gaaatctgtt aacgacgtga aagctgacga gcagatcctg gatatcggtg
    10681 atgcttccgc tcaggaactg gctgaaatcc tgaagaatgc gaaaaccatt ctgtggaacg
    10741 gtccggttgg cgtgttcgaa ttcccgaact tccgcaaagg tactgaaatc gtggctaacg
    10801 ctatcgcaga cagcgaagcg ttctccatcg ctggcggcgg cgacactctg gcagcaatcg
    10861 acctgttcgg cattgctgac aaaatctcct acatctccac tggcggcggc gcattcctcg
    10921 aattcgtgga aggtaaagta ctgcctgcag tagcgatgct cgaagagcgc gctaagaagt
    10981 aaaaaatcac agggcaggga aacctgccct tgtttcagcg cgcttttaga gcacgcattt
    11041 tttcatactc taaataattc gagttgcagg aaggcgacaa gagagtgaat ccccaggagc
    11101 ttacataagt aagtgactgg ggtgagcgaa cgcagacgca gcacatgcaa cttgaagtat
    11161 gacgagtata aggcccgacg atacaggaca agagacatgt ctaagatttt tgatttcgta
    11221 aaacctggcg taatcactgg tgatgacgta cagaaagttt tccaggtagc aaaagaaaac
    11281 aacttcgcac tgccagcagt aaactgcgtc ggtactgact ccatcaacgc cgtactggaa
    11341 accgctgcta aagttaaagc gccggttatc gttcagttct ccaacggtgg tgcttccttt
    11401 atcgctggta aaggcgtgaa atctgacgtt ccgcagggtg ctgctatcct gggcgcgatc
    11461 tctggtgcgc atcacgttca ccagatggct gaacattatg gtgttccggt tatcctgcac
    11521 actgaccact gcgcgaagaa actgctgccg tggatcgacg gtctgctgga cgcgggtgaa
    11581 aaacacttcg ctgctaccgg caagccgctg ttctcttctc acatgatcga cctgtctgaa
    11641 gaatctctgc aagagaacat cgaaatctgc tctaaatacc tggagcgcat gtccaaaatc
    11701 ggcatgactc tggaaatcga actgggttgc accggtggtg aagaagacgg cgtggacaac
    11761 agtcacatgg acgcttctgc actgtacacc cagccggaag acgttgatta cgcatacacc
    11821 gagctgagca aaatcagccc gcgtttcacc atcgcagcgt ccttcggtaa cgtacacggt
    11881 gtttacaagc cgggtaacgt agttctgact ccgaccatcc tgcgtgattc tcaggaatat
    11941 gtttctaaga aacacaacct gccgcacaac agcctgaact tcgtattcca cggtggttcc
    12001 ggttctactg ctcaggaaat caaagactcc gtaagctacg gcgtagtgaa aatgaacatc
    12061 gataccgata cccaatgggc aacctgggaa ggcgttctga actactacaa agcgaacgaa
    12121 gcttatctgc agggtcagct gggtaacccg aaaggcgaag atcagccgaa caagaaatac
    12181 tacgatccgc gcgtatggct gcgtgccggt cagacttcga tgatcgctcg tctggaaaaa
    12241 gcattccagg aactgaacgc gatcgacgtt ctgtaagtca ttcctttctg cttatctcaa
    12301 ggcccgctct gcgggtcttt ttttcgccaa aagaaaaatt gcctgatgcg ctacgcttat
    12361 caggcctaca cgtatcctgc aatatattga attcgcaggt ttttgtaggc cggataaggc
    12421 gttcacgccg catccggcat gacaaagcgc acattgatat ctgtctgagc cccgctctgc
    12481 ggggattttt tcgccaaaag caaggtgata cagatgagaa atctgtgatc tatttggcaa
    12541 aattatgctt tattgtttac ccttgtcaga ctgcccgtca taaggcggcg gagtgtattt
    12601 ctccattttg agtcagttga aaaggaatat tgaatggaag atttgaatgt tgtcgatagc
    12661 ataaacggcg cgggaagctg gctggtggct aaccaggcgc tgctgctaag ttatgcagta
    12721 aacatcgtgg cggcactcgc gatcatcatc gttggtttga ttatcgcgcg gatgatttcc
    12781 aacgcggtga atcgcctgat gatctcccgt aaaatcgatg ccactgttgc tgattttctt
    12841 tctgcattag tccgttacgg tattatcgcc tttacgctaa tcgctgcact gggacgcgtg
    12901 ggtgtacaaa ccgcgtccgt cattgctgta ctcggtgccg caggcttagc tgttggtctg
    12961 gctttgcagg ggtcactgtc taacctggcc gctggcgtgt tacttgtcat gttccgcccg
    13021 ttccgtgccg gagaatatgt tgacctcggc ggcgtagccg gtactgtgct gagtgtgcag
    13081 attttctcca ccaccatgcg tactgcagac ggtaaaatta tcgttattcc gaacggtaaa
    13141 attattgccg gaaatattat taacttctcc cgcgagccag ttcgccgtaa cgagtttatt
    13201 attggcgtgg cgtatgattc cgatatcgat caggttaagc agatcctgac caatattatc
    13261 cagtctgaag atcgcatttt gaaagatcgc gaaatgactg tgcgcctgaa cgaacttggt
    13321 gcatcgtcga ttaatttcgt ggtccgcgtc tggagcaaca gtggcgatct gcaaaacgtg
    13381 tactgggatg tgctggagcg tattaaacgt gaatttgatg ccgccggtat cagcttcccg
    13441 tacccgcaaa tggatgtgaa ctttaagcgg gtgaaagaag acaaagctgc gtaatcaacg
    13501 ctgaggccag ataatactct ctggcctctc tcttattagt ttttctgatt gccaattaat
    13561 attatcaatt tccgctaata acaatcccgc gatatagtct ctgcatcaga tacttaattc
    13621 ggaataccaa acgtgttttc ttattacttt caaggtcttg cgcttggcgc agctatgatc
    13681 ctaccgctcg gtccacaaaa tgcttttgtg atgaatcagg gcattcgtcg tcagtaccac
    13741 attatgattg ccttactttg cgctatcagc gatttggtcc tgatttgcgc cgggattttt
    13801 ggtggcagcg cgttattgat gcagtcgccg tggttgctgg cgctggtcac ctggggcggc
    13861 gtagccttct tgctctggta tggttttggc gcttttaaaa cagcgatgag cagtaatatt
    13921 gagttagcca gtgccgaagt cctgaagcaa ggaagatgga aaattatcgc caccatgttg
    13981 gcagtgacct ggctgaatcc gcatgtttac ctggatactt ttgttgtact gggcagcctt
    14041 ggcggtcagc ttgacgtcga accaaaacgt tggtttgcgc tcgggacaat tagcgcctct
    14101 ttcctgtggt tctttggtct ggctattctc gcagcctggc tggcaccgcg cctgcgcacg
    14161 gcaaaatcac agcgcattat caatctggta gtgggatgtg tcatgtggtt tatagccttg
    14221 cagctggcga gagacggtat tgctcatgca caagccttgt tcagttaggc gctatctgat
    14281 ggaaaaataa aacagaggcg ctaagcttgc ctccagaggt cctgaatttt aggcaagtca
    14341 aataacacgg agagactaac gtgaagttca aagttatcgc cctggcggca ttaatgggta
    14401 ttagcgggat ggcagcgcag gctaacgaat tgccggatgg accgcatatt gtcacctccg
    14461 gtacggcaag cgtggatgcg gtgccagaca ttgccactct tgcgattgaa gtcaatgtgg
    14521 ccgcaaagga tgccgctact gccaagaaac aggcggatga gcgcgtcgca caatacattt
    14581 ccttccttga actcaatcag atcgcgaaaa aagatatcag ctcagcgaac ttacgcaccc
    14641 agccagatta tgattatcag gatggtaaaa gtatccttaa aggctaccgc gctgtgagaa
    14701 cggtggaagt cacgctccgt cagttagaca aactgaattc cttgctggat ggcgcgctga
    14761 aggcgggtct taacgaaatt cgttctgtgt cgctgggcgt ggcgcagccg gatgcctata
    14821 aagacaaagc acgtaaggca gcgattgata acgcgattca tcaggcgcag gaactggcga
    14881 acggctttca tcgtaaactg gggccggtat atagcgtgcg ctaccatgtt tccaactatc
    14941 agcccagccc aatggtgcgg atgatgaaag ccgatgccgc gccggtctca gcccaggaaa
    15001 cttacgagca ggccgctatt cagtttgatg atcaggtcga tgtggtcttc cagttagaac
    15061 ctgtggatca acaacccgct aaaacacctg cagcacaata atacttgtag cccgacgtat
    15121 tcgtcgggct aatgccttta cccgatattg cctccttttt ctgtaatttt ctgtaaaacc
    15181 atgacgtaat cacctatctt tgctcgcggt ttcatcaagg ataggctgac tgaaacggtt
    15241 aagcggacaa ggatgcgggg gaggggggat ggacattttt atttcgaaaa agatgcgcaa
    15301 tttcatttta ttagcgcaaa ccaataatat tgcccgggcg gcagaaaaaa tccatatgac
    15361 ggcttcgcca tttggtaaaa gcattgccgc gctggaagaa caaattggct atacgctatt
    15421 tacccgcaaa gataataata tcagcctcaa taaagccgga caggaactgt atcaaaaact
    15481 gttcccggtt tatcaacggc tttctgcgat tgataatgaa atccataact cagggcgtcg
    15541 ttcacgagaa attgtgattg gcatagataa tacttatccg acaattattt tcgatcagtt
    15601 gattagtctc ggagataagt atgaaggcgt gaccgcccag ccagttgagt tcagcgaaaa
    15661 cggcgttatc gataatcttt ttgaccgcca gttagatttt attatctccc cacagcatgt
    15721 atctgcacga gttcaggaac tggaaaacct gactatcagt gaactaccac cgctgcgatt
    15781 gggctttctc gtttctcgcc gttatgaaga gcgtcaagag caagagctgc ttcaggagct
    15841 gccctggcta caaatgcgct ttcaaaaccg ggctaacttc gaagcaatga ttgatgcgaa
    15901 tatgcgacct tgtgggatca atccaaccat tatttatcgt ccctacagtt ttatggccaa
    15961 aatcagcgcc gtggagcgcg gacattttct gacggtgatc ccgcattttg cctggcgact
    16021 ggtgaatccg gcaacgctga aatattttga tgcgccgcac aggccgatgt atatgcagga
    16081 atatctttat tcaatcagaa atcatcgtta taccgccacg atgcttcagc atattgctga
    16141 agatcgtgac gggacaaacc attaacccag catcgagccg gttgcaatta aattacggtg
    16201 taagtcgaag acgtggctaa gatcgtggtg aatatgtccg ccaggcgcat tttccagata
    16261 gcgatgcaga taatcccgat acataggatg tgcacaatta tcaatgatcg tgcgggcgcg
    16321 ttgaagcggg gaaagaccgc gcagatcggc gatcccttgt tcggtgatga tcactttgac
    16381 gctgtgttcg ctgtgatcaa catggctgca cattggcacg acggttgaga tcttgccttc
    16441 tttagcaatc gatggggcca taaagatcga cagatacgcg ttgcgttcaa aatcaccgct
    16501 gccgccgatg ccgttcatca gatcgacccc ggctacgtgc gttgagttgg catgcccgta
    16561 aatatcaaac tccaggccga cgttcagagc gatgacgccc agacgacgga tgatttccgg
    16621 gttattggaa atctcctgcg gacgcaacac aatgcggctg gcaaagtaat ccatattgtc
    16681 gtaaatcttg cgcagggaat cggccgagat tgtcaggctg gaggcgctgg ccccgctgat
    16741 tttgccggtt tccagtaaat gcaccaccga ttcctgtagc acttccgaat acatcataaa
    16801 cggaggaatt tccgggtttt cccccagacg cgccattacc gcattattga tattgcccac
    16861 gccactttgc agcggcagaa attccggcgg aatacgccca tgcgccattt cctgcaataa
    16921 gaacgtgacc acgttatcgg caatctgctg gcacatggga ttttgcttat ccagcatatt
    16981 accggcgtcg ggcaagttgg tttccacgac ggcgacaatc tttttcggat cgatttgcac
    17041 atagcgggta ccgacgcgat ccattgcatg gaagatcgac acgctattac gccgcggtgg
    17101 cgcgccagga atcacaatat ctgccagttc tgcaacgcgc ggatcgtgat agtgattaag
    17161 ttcaatgatc actttcttcg cccgcagcag ccatgtcggc gcattaccga tcccgctggt
    17221 taaccagact cgaccatccg gtgccagtgc cgatgcttca atgacggcaa catcaatgtc
    17281 gccgaagaaa ccgtaattga ccatttgcgc cacttcgctc aaatgcaggt caacgaaact
    17341 caccgcgccc tgattgatct ttttacgtaa accggacgat gtttgatatg gcgcacgcca
    17401 ggaaacagca tcagcgtcag aaagtacatc gtcagcggcg gcgctgattg acgcacccgt
    17461 cagcaggcga atttgatacg gctttttggc ctcatgctgt tcgttagctc tgcgggcaat
    17521 cgcggtgggt agggctttcg gtgaacccgc tggggtaaag ccgctaaatg ccaccatgtc
    17581 gttatgctgg ataatttctg ccgcttcatc ggcggtcatc cttgtccact gagtttccat
    17641 ttttactcct tcgttcgcag ggattaatga ccaacgaaat taggtttacg tttttcgagg
    17701 aaagcgttca tcccttcctg gtaatcttcg ctgtcataaa cggcgcggcg catcccctga
    17761 atacgttcaa attcatcgga gttcatggtg tgtgcttcgc ccagtacacg cagctcttct
    17821 ttgataacgg caatggctaa cggcgctttc tcagagatgt ggtgcgccat ttgtaaggtg
    17881 aaatcttcca gttcttccac ttccacaaca tggttgagga tgccgacagc cagcgcgcgc
    17941 tgggcggtga ttggcgaagc ggtaaaaatc agctctttga caatgtggaa gcccgcgtcg
    18001 cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc cgaggtttac aggtgtcatt
    18061 gagaaggttg aggtactggc ggcgatgatc agatcggaac tcatgatcat ttcaaatgcg
    18121 ccaccccaaa cactaccttc caccatcgaa atgatcggtt tcgggaattt ttggatcatg
    18181 cgggtgattt gacgcaacgg atcatcatag gagagcggat cgcgaccgcc agacggcagt
    18241 tcgtgaatat cgtgacctgc ggagaagact ttggatccac tcggcgcgcg caaaatgata
    18301 cagcgaattt ccggtcggtt gagatcgctt aatgcctgca taagatcatc aataaagact
    18361 ttacttaagg cattaagttt gcggccatag ttaaactcaa tgaccgccac tttgttgata
    18421 gtgacaacgt tcacatactg ataagacata aaaattcctt taatcaaaat attgcgtctg
    18481 gataaattca ctgagctgcc gcaggccggt gcgcggtgag agcgtattgt ttttgaccgc
    18541 taaaagcgtc tggcggtaat agcgatcgaa atcttcattc gcgaacaggt gattcagtac
    18601 ttcttcttcg gtttgtttac gcagccattc caccgattgt tgttgccgca cttgctgtaa
    18661 acgaccactg gcagttagcg cggttttgaa gtcgatgatg gcgtgccaga tctcatcgat
    18721 tccacgtttt tccagtgcgc tacaagtcag aacccgtggc tgccattcgt cgtatttacg
    18781 tcgcagaata tgcagggcac tctcgtacat atgccgggca atggcgacat tggtatggtt
    18841 atcgccatcg tctttgttga taacgatcag atcagccact tccatcagcc cttttttaat
    18901 gccctgcaga tcatcgccgc caccggcaat ttgcaacgag ataaaacagt ccaccatgcg
    18961 ggcgacttcc gtttccgact gcccgacgcc aaccgtttcg acaatcacta cgtcataacc
    19021 cgccgcttcg cataacagca ttaattcccg cgctcgctga ctggcaccgc ccagatgacc
    19081 ggaggatggt accgggcgaa taaacgccgt ctcggcacgc gccaggtcat tcatgcgggt
    19141 tttatccccg agaatgctac cgccagtgac cggactgctg ggatcgaccg caataaccgc
    19201 aacttttaat ccatcacgaa tcaacaacat gccaaaggct tcaagaaagg tacttttccc
    19261 cgcgccgggt gtgccggtaa cgcccagtcg cagggcgtta ccgcagtacg gcataatggc
    19321 atcaagcagt tgcgtactta gcgcctgatg acgcgggtga cggctttcca ccagcgtcat
    19381 ggcctgggcg agtgtggcac gctcaccctg acgtaagcgg cgaatacttt ctgccagcgt
    19441 ggcttcatta atcatgatgc tggcttatca gattcagtac gtcgcgcaca ctgtcgagca
    19501 taggtgtacc tggaccataa atcgccgcca cgccgcgctc ttgcaggaag gcgtaatcct
    19561 gcggtggaat gacgccaccg gcgactacgc agatatcttc gcgtccccat tttttcagcg
    19621 cttcgaccag ttccgggatc agcgttttat gaccggcagc cagtgaggat gcgcccacta
    19681 cgtgaacgtc attttctact gccaggcggg cgatctcttc aggtgtagag aacatcgggc
    19741 ttaaatctac gtcgaaaccg agatcggaat aggcgctggc gatcactttt gcgccgcgat
    19801 cgtgcccatc ctggcccatt ttggcgatca gaatgcgcgg gcgacgacca ttgtcggcaa
    19861 ggaactgctc cgtttgcgca acaatggcat cgaactcgga ggccgatttc tcagattgat
    19921 gatagctttg cgcaattacg ccggtaacac actggcttgg caccagataa cggtcgaaag
    19981 cggcttccag cgcatcggaa atttcaccca gggtggcgcg aacgcgagcg gcattaacag
    20041 cggcagccag caggttttcg ttatgctgtg cggcgtgagt cagggcgttc aacgcggcgg
    20101 ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag cgaagcaatt tgctcgttac
    20161 gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc ttcgtgatcc agtttgtact
    20221 tgttgacacc aacgatgaca cgcttgccct ggtcgatcag cgactgttcg cgcgctgagg
    20281 cctcttcgat cattcgtttt ggcagacctg cttcgatcgc tttcgccatg ccaccggctt
    20341 cgtcgatctg ttggataata actctggctt gtttgacgat ttgatcggtc agcgactcaa
    20401 tgtaatagga tccggccagt ggatcgacgg tgcggcagag ttctgattct tcctggatga
    20461 tgatctgggt gttgcgggca atgcgtgctg agaaatcggt aggcaaacca agcgcttcgt
    20521 caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt cgcagccagt gcttcaatgg
    20581 tggtgcggat aacgttgtta tacggatcct gttcagtcag gctccagcct gaggtctggc
    20641 agtgggtacg cagcgccagt gatttcgggt cctgtgcgcc aaatccactg accgcttcgc
    20701 tccataaata acgtgccgca cgcaacatgg cgacgttcat aaacagatcc atgccgatgc
    20761 cgaagaagaa cgacaggcga ggagcgaagt catcaatttt cagtccggca gagattgctg
    20821 ctttgatgta ctcaatccca tcagcgagcg taaatgctac ctgctgcacg cagttggcac
    20881 ccgcttcacc catgtggtaa ccgctgatac tgatggtatt aaatcgcggc atgttgccgg
    20941 aacaccaggc gatgatgtcg gcgataatgc gcattgacgg ttttggcggg taaatatagg
    21001 tgttgcggca gaggtactct ttgaggatat cgttctgaat ggtgccggtc agtttatcag
    21061 gtgtaacacc ttgctcttcc gcggcgacga tataaaacgc cagtactggt agcactgcgc
    21121 cattcatggt catcgaaacc gacattttat ccagcgggat ctggtcgaac aggactttca
    21181 tatcttccac ggtgtcgata gcgacgcccg ctttgccgac gtcgcccgcc acgcgcgggt
    21241 tatcggagtc gtagcctcgg tgggtggcaa ggtcaaacgc aacggaaaga cctttttgcc
    21301 cggcggccag gttacggcga taaaaagcgt tggactcttt tgctgttgaa aaaccagcat
    21361 actgacggat ggtccacggt tgggcggtat acatagtggc acgcgggcca cgaacgtagg
    21421 gcggcaaacc aggaagggta cctgtcacct ccagattatc gagatcggct tcggtataca
    21481 gcggcttgat ggcgatccct tccgcggttt gctgaaccag cgagtcgaca gttttctccc
    21541 gacggctcaa ttccttgttg gcaagctgtt gccactcctg cacgttagac attatttgct
    21601 ccgtaaaacg caatcaaaat gagggtattt gcgcctacgg tgagcttttt acgtgggttt
    21661 gtcgccagca aaaaaaatgc attcagacgt cgttttttgc cgcctgagat ttgtctattt
    21721 tgtgatgtga ttcaaggaat acaaaaagaa gtgaggacaa cgcctgatat gtgctatcag
    21781 gcattatttg atggattaat cctgacgaag gactttgtga ccataatcga gtaacgcatc
    21841 agtgacttta cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga gcatccgtcg
    21901 ttgaaatagc ccaggcgtta agtcaatcag ttcaccgctg gccagctctt tctcgatttg
    21961 caggtgcggg atcatacagc aggtggtgcc ctggcgagca agttgcacga acgcttctga
    22021 agaattaacg atatggcagg gtacgctgcc aggaggcaga tcgaagtttt gctgcaaaaa
    22081 ggcctggtgc atatcgtcaa gatggtcaaa cgcgaccact ggcgctttca gtaatgccga
    22141 acgcgttacg ccgttaggga aatatttttc ggcaaacggt tttgagctga cgaacagata
    22201 gtcgagcgca ccaagtttat cgacaagaca actcggcagc gcctgatgtt gaatactcac
    22261 cgcgccgacc acttcgccgc gacgcagacg ttcctgagtg cgggtttcat cttctacctg
    22321 caagttgagg cggataggcg aatcagccaa cacaggagcc agtgccggaa gcaaccacgt
    22381 cgccagactg tcggcgttga ccgccagtga aagcagcagc ggagtcgaac cggtttgttc
    22441 atcgcccagc cactcttctt ccagcaactc cacctggcgc agcagtgcca gcagtttttg
    22501 cccttgttcc gtcgggcgcg gcggtacggt acgcaccaac agcggctgcc cgaacatatt
    22561 ttccagttgc ttaatgcgct gtgagacggc tgattgtgta atgcacagct tttgtgccgc
    22621 gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc tgtagtccgg
    22681 gcgtttcatt gctgttgtct gctcctggta aatgcttttt cctgcactat gacataattt
    22741 tgcgttgggt acagagcatt taacgcatac aaaagcggtc gtaatggaag ttttggctgg
    22801 caatcccatt ttcatataac cctttgctga gcgctttggc aaaactctgt gggccgcaaa
    22861 accagaaatg ggtgtcacct ttatttgcta ttttatcagt aagatagtgc gactgtaggc
    22921 gctcaccttt atgcgcatca agcagatgca gtgtgacact ggcctgggca caaagtgagg
    22981 taagtttatc aacataccat gcatcctcgc ttcggctgac gcaataccag agatccacag
    23041 gaacacggga cgcgccatgc tggttcagat actcaagctg ggcgataaaa ggggtaatgc
    23101 cgatgcctcc tgctatccag acctggcggg agcaagggag agtaaaatca aactttcccc
    23161 atggcccttc aacgagggcg gactggccga tttgtatcga ggatgccaga gtcgaggtat
    23221 aatcccctaa atttttgatg gcaaagcgca acgtgttctt ctcttttaca tatgaagcga
    23281 tagtaaaagg gtgagattct ttactgtgtg cgaacgttaa gaatgcaaac tgccccggac
    23341 aatagtcgat gggatgttcc atctccagta tgacttcggt aatttgtccc ggatggttaa
    23401 caatgctttt aacggtggcc tgatgcttgc ttttattacc gtttaatttc agcagcgaaa
    23461 tgaaagccac aaagacgcct gggatagtaa ttaaaattaa taaatacgca gcaggagttt
    23521 cccaccaata tgatttaaac agtgcagtaa atacatgaaa agcaattgcc agataaaaaa
    23581 cggggaacaa acggtgaaca agatgaaata aatgataggg aacttttttg cttagagaca
    23641 cgaaaaccat cgctatcata ataaacattg cacattcacc cgctaacaat cccgcatgaa
    23701 gccaattttc ttgtactggc gtcaggttaa tatcaacgat tttaccaggg tgagtaagcc
    23761 agccaaaggc gacgaataac tggggtaatt tttcatccag ccagtgggcg aaagcaaaag
    23821 cgatggcgca tattcctgcc cacttatgta atcgccatgc tttatccaga ccgcccagca
    23881 ctttattaag ccatgatgaa cgcagcgata aacacatcgt cagcgtcatc agacaccacg
    23941 caagggttcc cgtaaaaaag agcgtgtttt gtcgaatgaa ccatattagc gcgattggct
    24001 tttcacgaaa aatatcgctg gacgacaaaa aaataataca tgcgacaaag caaaccaacg
    24061 tacctgtaag attagctttt ttcatttttt atttcatttt ggcagcggag catagaacag
    24121 ttccatgctc ccgttaaata gagtgattat ttat
//
LOCUS       DAZKFU020000048        23951 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZKFU020000048 DAZKFU020000000
VERSION     DAZKFU020000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.048"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..951
                     /gene="aaeR"
                     /locus_tag="ACHOQQ_RS07685"
                     /old_locus_tag="pgaptmp_001537"
     CDS             22..951
                     /gene="aaeR"
                     /locus_tag="ACHOQQ_RS07685"
                     /old_locus_tag="pgaptmp_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGU4469805.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1079..2524)
                     /gene="tldD"
                     /locus_tag="ACHOQQ_RS07690"
                     /old_locus_tag="pgaptmp_001538"
     CDS             complement(1079..2524)
                     /gene="tldD"
                     /locus_tag="ACHOQQ_RS07690"
                     /old_locus_tag="pgaptmp_001538"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGU4469806.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGAMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2863..6663)
                     /gene="yhdP"
                     /locus_tag="ACHOQQ_RS07695"
                     /old_locus_tag="pgaptmp_001539"
     CDS             complement(2863..6663)
                     /gene="yhdP"
                     /locus_tag="ACHOQQ_RS07695"
                     /old_locus_tag="pgaptmp_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGU4469807.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6731..8200)
                     /gene="rng"
                     /locus_tag="ACHOQQ_RS07700"
                     /old_locus_tag="pgaptmp_001540"
     CDS             complement(6731..8200)
                     /gene="rng"
                     /locus_tag="ACHOQQ_RS07700"
                     /old_locus_tag="pgaptmp_001540"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGU4469808.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8190..8783)
                     /gene="yhdE"
                     /locus_tag="ACHOQQ_RS07705"
                     /old_locus_tag="pgaptmp_001541"
     CDS             complement(8190..8783)
                     /gene="yhdE"
                     /locus_tag="ACHOQQ_RS07705"
                     /old_locus_tag="pgaptmp_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGU4469809.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8792..9280)
                     /gene="mreD"
                     /locus_tag="ACHOQQ_RS07710"
                     /old_locus_tag="pgaptmp_001542"
     CDS             complement(8792..9280)
                     /gene="mreD"
                     /locus_tag="ACHOQQ_RS07710"
                     /old_locus_tag="pgaptmp_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGU4469810.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9280..10383)
                     /gene="mreC"
                     /locus_tag="ACHOQQ_RS07715"
                     /old_locus_tag="pgaptmp_001543"
     CDS             complement(9280..10383)
                     /gene="mreC"
                     /locus_tag="ACHOQQ_RS07715"
                     /old_locus_tag="pgaptmp_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGU4469811.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10449..11492)
                     /gene="mreB"
                     /locus_tag="ACHOQQ_RS07720"
                     /old_locus_tag="pgaptmp_001544"
     CDS             complement(10449..11492)
                     /gene="mreB"
                     /locus_tag="ACHOQQ_RS07720"
                     /old_locus_tag="pgaptmp_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGU4469812.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11797..13737)
                     /gene="csrD"
                     /locus_tag="ACHOQQ_RS07725"
                     /old_locus_tag="pgaptmp_001545"
     CDS             complement(11797..13737)
                     /gene="csrD"
                     /locus_tag="ACHOQQ_RS07725"
                     /old_locus_tag="pgaptmp_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGU4469813.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPTTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13889..14863
                     /locus_tag="ACHOQQ_RS07730"
                     /old_locus_tag="pgaptmp_001546"
     CDS             13889..14863
                     /locus_tag="ACHOQQ_RS07730"
                     /old_locus_tag="pgaptmp_001546"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGU4469814.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15841..16311
                     /gene="accB"
                     /locus_tag="ACHOQQ_RS07735"
                     /old_locus_tag="pgaptmp_001547"
     CDS             15841..16311
                     /gene="accB"
                     /locus_tag="ACHOQQ_RS07735"
                     /old_locus_tag="pgaptmp_001547"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGU4469815.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16322..17671
                     /gene="accC"
                     /locus_tag="ACHOQQ_RS07740"
                     /old_locus_tag="pgaptmp_001548"
     CDS             16322..17671
                     /gene="accC"
                     /locus_tag="ACHOQQ_RS07740"
                     /old_locus_tag="pgaptmp_001548"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGU4469816.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            17780..18022
                     /locus_tag="ACHOQQ_RS07745"
                     /old_locus_tag="pgaptmp_001549"
     CDS             17780..18022
                     /locus_tag="ACHOQQ_RS07745"
                     /old_locus_tag="pgaptmp_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGU4469817.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            18012..19463
                     /gene="panF"
                     /locus_tag="ACHOQQ_RS07750"
                     /old_locus_tag="pgaptmp_001550"
     CDS             18012..19463
                     /gene="panF"
                     /locus_tag="ACHOQQ_RS07750"
                     /old_locus_tag="pgaptmp_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGU4469818.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFIGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            19475..20356
                     /gene="prmA"
                     /locus_tag="ACHOQQ_RS07755"
                     /old_locus_tag="pgaptmp_001551"
     CDS             19475..20356
                     /gene="prmA"
                     /locus_tag="ACHOQQ_RS07755"
                     /old_locus_tag="pgaptmp_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGU4469819.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMTDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            20685..21650
                     /gene="dusB"
                     /locus_tag="ACHOQQ_RS07760"
                     /old_locus_tag="pgaptmp_001552"
     CDS             20685..21650
                     /gene="dusB"
                     /locus_tag="ACHOQQ_RS07760"
                     /old_locus_tag="pgaptmp_001552"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGU4469820.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            21676..21972
                     /gene="fis"
                     /locus_tag="ACHOQQ_RS07765"
                     /old_locus_tag="pgaptmp_001553"
     CDS             21676..21972
                     /gene="fis"
                     /locus_tag="ACHOQQ_RS07765"
                     /old_locus_tag="pgaptmp_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGU4469821.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            22058..22942
                     /gene="yhdJ"
                     /locus_tag="ACHOQQ_RS07770"
                     /old_locus_tag="pgaptmp_001554"
     CDS             22058..22942
                     /gene="yhdJ"
                     /locus_tag="ACHOQQ_RS07770"
                     /old_locus_tag="pgaptmp_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4469822.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLSTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            23026..23205
                     /locus_tag="ACHOQQ_RS07775"
                     /old_locus_tag="pgaptmp_001555"
     CDS             23026..23205
                     /locus_tag="ACHOQQ_RS07775"
                     /old_locus_tag="pgaptmp_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGU4469823.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(23208..23870)
                     /gene="acrS"
                     /locus_tag="ACHOQQ_RS07780"
                     /old_locus_tag="pgaptmp_001556"
     CDS             complement(23208..23870)
                     /gene="acrS"
                     /locus_tag="ACHOQQ_RS07780"
                     /old_locus_tag="pgaptmp_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HGU4469824.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         5765 a         6381 c         6031 g         5774 t
ORIGIN      
        1 acttttaagt cagagtgaat aatggaacga ctaaaacgca tgtcggtgtt tgccaaagta
       61 gttgaatttg gctcttttac cgccgccgcc agacagctac agatgagcgt ttcgtccatc
      121 agtcagacgg tgtcaaaact ggaagatgag ttgcaggtaa agctgttaaa ccgtagcaca
      181 cgcagcattg gcctgaccga agccggtaga atttactacc agggctgccg tcgtatgctt
      241 catgaagtgc aggatgttca tgagcaactg tatgccttca ataacacccc catcgggacg
      301 ctacgcattg gctgttcttc aactatggca caaaatgttc tcgccgggct gacagccaaa
      361 atgctgaaag aatacccagg tttgagcgtc aatctggtta ccggaattcc agcccccgac
      421 ctgattgccg acggtctgga tgtggtgatc cgcgtcggcg cgttgcagga ttccagcctg
      481 ttttcccgcc gtcttggcgc gatgccaatg gtggtgtgcg ccgcgaaaag ctatctcaca
      541 caatacggca taccggaaaa acccgccgat ttaagtagcc attcatggct tgaatacagc
      601 gtgcggcccg acaatgaatt tgaactgatt gcaccggaag ggatctcgac tcgcctgatc
      661 ccgcaaggga gatttgtgac taatgatccg atgacgctgg tgcgctggct aacggcgggt
      721 gccgggatcg cctacgtgcc gctgatgtgg gtgatcaacg agatcaatcg tggggagctg
      781 gagatcctgc tgccgcgtta tcagtcagat ccacgcccgg tttatgcgtt atataccgaa
      841 aaagataagc tgccgctgaa ggtacaggtc gtgatcaact cgctgacgga ttattttgtt
      901 gaggtcggta aattgtttca ggagatgcac gggcgcggga aagagaagta attcatcttg
      961 tactgttacg ttgtacaaac ctgtgccaac gggttcccct caccctaacc ccagaggagc
     1021 gaggggaccg ttcgtgcacg tagaaagatt aattatcctt ctgaaaatac tgaaattatt
     1081 acgcagtacc gccaacagtc aggttatcga ctttcaacgt tggctggccc acgccaaccg
     1141 gcaaactttg cccttcttta ccgcagacac ccacgccgtt atccagtttc aggtcgttgc
     1201 caaccatcga aatctgctgc atggtttcga taccggaacc aatcaacgtt gcgcctttca
     1261 ccggcttcgt tactttaccg ttttcaatca gatatgcttc tgaagtggag aaaacgaatt
     1321 tgccggaggt gatatccacc tgaccgccac caaagttcgg tgcatagata ccgtactcaa
     1381 cggattcaat aatttcctgc ggggtcgatt tacccggcag catataggtg ttggtcatac
     1441 gcggcatggg cagatgggcg taggattcac ggcgaccgtt gccagtcggc gtcatcccca
     1501 tcaaacgcgc gttgagttta tcctgcatgt agcctttcag aatgccgttc tcaatcagca
     1561 cgttgtactg gcctggcgta ccttcgtcat caatcgccac cgaaccacgg cgatcgacca
     1621 tcgcgccgtc atcaaccacg gtacacagtt ctgaagccac cagctccccg acctgtccac
     1681 taaatactga agtgccacgg cggttgaagt cgccttccag accgtgacca accgcttcat
     1741 gcaacagcac gcccggccaa cctgcgccaa gtactaccgg catggtgccc gctggtgcag
     1801 caacggcaga aagattgacc agcgccatac gcactgcttc ttttgcccat gcatccgcac
     1861 ggacttcgcc gtcgagatcg gcaaggaaga actcataacc aaaacgaccg ccgccgccac
     1921 tggcaccgcg ttcgcgtttg ccatcttctt cgaccagaac gctcacggaa agacgcacca
     1981 gcggacggac atccgccgct agcgtgccgt cggtggccgc aaccaaaatt aattcataga
     2041 caccactgag gctggcagtc acttcctgta cgcgcttgtc cgcttcgcgg gcaaccttat
     2101 cgacgcgacg caggatatcc agcttctctt cacggctcat gctttgcagc ggatctaccg
     2161 aggtatacaa cgggctatgc tctaccgcgc ccagcgtctg tactttgcca tcaccactat
     2221 cacggacgat ggtgcgcgcc gcttgcgcac tctgttccag cgccagcagg ctgatttggt
     2281 cagcgtaagc aaatccggtt ttttcaccgc tgattgcacg cacgccaacg ccctgatcga
     2341 tgttgtaaga accatcttta ataatgcggt cttctaaaac ccaggattcg tgatagctcg
     2401 actgaaaata gagatcgcca taatcaaggc gacgttcggc cagttgaccg aggatcgcga
     2461 acaagtcctg atgtttcagg ccgttcgccg ctagcaattg ttcacttacc aggttcagac
     2521 tcatcgtttt tgctactcgt tagttactgc agtagaggat tttttacggc tgccggatgc
     2581 ggcgtaaacg ccttatccgg cctacgatta tgttccggtt tgtaggcctg ataagacgca
     2641 cagcgtcgca tcaggcaacg gctgtcggat gcggcgtaaa cgccttatcc gacctacggt
     2701 tatgttccgt ttgtaggcct gataagacgc acagcgtcgc atcaggcaac ggctgccgga
     2761 tgcggcgtaa acgccctatc cggcctacgg ttatgttctg gctcgttggc ctttggcaac
     2821 gattatctta tgagattggg gcaattacgc gccctcgtca aatcattgcg ctttttcttt
     2881 acgcggttgg cgcaacactt cgttgatttg cggatcgtcc agcggacccg aaatgtgata
     2941 gcgcaaaatg gagactttgc tccacagcgg ccccagcact ttactggcag caaacactgc
     3001 cgcgccaaca atggggttaa ccgcaaaagc cgcagccacg cccaccgtcg cagaaatctc
     3061 tggtgcgaca accgcttcca tattcaggtc gcgacgtacc agatttaccg accctttcat
     3121 ggcgatatcc gcctccaggc catccaccaa cgtgtcgtcg gtgtgcataa cgccgtcttt
     3181 aatccacgcg gtgctgcgaa tggagtcaaa atagaaccct tcgccaaaag tgtctctgaa
     3241 atcaaaacgc agcttacgca tcagggcatc tacgctcaat aagcgcagca attgcccggc
     3301 atgtccggta ttgatttcgg taatttcgcc tttacccagt tgagtatgaa tgatgccatt
     3361 caacgtcgcc tcatctggct gccacggtgc tttacgccag tgtaaatcgt aatccacatt
     3421 aaatgacgac tgccgtatgg gcgtcgtgac accaaaaaat tctgcggcgg catcaatttt
     3481 ctgcccgcgc agttttcctt tcagcgaggt acgttcattc cccggattat taacccattc
     3541 accatcggca gtaagccgcg agaaaccggt atcaatcagt ccattggtca gcgttaatgt
     3601 atcgccagaa atggtgagat cactgtcaat acgaccgaat ttttgccccc agaaccagca
     3661 ctctgcgcat cgtatttgtg cgtccggcca gccacggaag ttaatgcgct ccgttgtcgg
     3721 gaaaggtgat gacggcgttg aatcaccacg agttttcgcc acgctcgggt tgtaataaag
     3781 atatttgata ttcgccagcc acggcgcgtt attacgcatc gccagcgtgg cgttgatttc
     3841 acgcccttgc gcctcaacct gggtgccatt tgccgtcggt tgcgaaacaa tactcaggtt
     3901 attccattgc tgatttccca gcgacaacat aggcgtacgt aacgttatgt gttgtgggaa
     3961 actcgctgca ccaccgacac tctccgccgc acctttctga aacagggcca gccactcggc
     4021 accattcatc ggcggcatat tgagttcaac accactttgt tccggcaacg gcgggagcgt
     4081 tttactgtct gccgcccaaa tagcacgatc gagcgtcagc ttttgaccga gcaaccagcg
     4141 gctattgaaa tggttatccg caccagcctg tccggttaat tcaaagctgt tgagattgcc
     4201 atcaacctta acgtttaccg ctagtggttc acccgcaggt ttggctaacg gtgaaggtaa
     4261 gtgactgctc acattcttca gatcgccgtt cagctctacg ttataggtcg cgccagcatg
     4321 ataaggcaga tcaatgccca ctttaccatc ccacgccacg ctgccactca atgcttcgtt
     4381 caccgctgca ggcagaacgc cggttttcgc tggttgccag ttaccgttga gattcaccgc
     4441 gacctggtag gcttttgccc cttctttggt ggaaaaatcc acgttcaacg gctgattaaa
     4501 ccagcttgct gtcagtggtt cactttgcag atcgccattg ataaagctga atttaccgct
     4561 caaatttttc agggtgctgt cgagtggttt gataaacaga ctgttattac gcagcgtcac
     4621 atcacctttc gcggttacca gttcgccgtt cagcgggata tcaagatgta agcgagcatt
     4681 cacatcgccg tcgagctgga gttcttgcag ggtcgcaccc agagaatctt tcagcggtgt
     4741 ctcatcaaag taagggccaa cggctttacc cggaccttta atgtcagcgt caatcagcag
     4801 tttttctttc gagtagtcag ggatcactgc ggtgagatta ctcgcgcgca cgccgcccag
     4861 attaacgcca tcggttttca tccataaacc gtcgttaata aagtccagtt caatatcaag
     4921 gttagttaat gcaggccagt ccggctggaa ggcaaacttc gcgttgcgca gcggcaccag
     4981 cacttcaaac tgaccttcgt tgtgtttata ggggaagagt tgcggattgc caccataaac
     5041 cagcgtcgcg ttatccgctt caccgccctg aatcgcgcca cttaagtaat caaccaggtc
     5101 tttacccatc aagttttccg ggaaatagcg ccaggcttgt gaaccatcat cggtactgat
     5161 gccagccaga atacccagcc agggttcatc gttagcaggt tgcaggtaac gaaaaccgcc
     5221 gcgcgcatgg acggctttgg ctttaacgtc aatattacgc ccatccagct ggaaaccttt
     5281 gtcattgttc agccagctta tagttgcctg gccgtcggcg atttccagtg gcgcacggaa
     5341 taccgtttcg taaggcatct ttgcctgctt catcgacgcg gtaagcaaac cattttcaac
     5401 gctgccggaa agcgtcccgg agaagtgttc cgcacccggt aataatttcc attgcttcca
     5461 ggccagatcg ctccacgatg cctgaaaacg ggtcttgtct gccgcctgaa gcgggatatc
     5521 cagcgccagc gtgttaatct tgccgctcgg ttgtgtggag cgccaaacat cacccagtgc
     5581 aggtgaaagt ttcgcggcca gcgggcgtat gccctccagg cctgccagct ccagattact
     5641 ggcgcgaatc cggagttcgt cactgcgttt attgtctttg ccgccaacgt cctgttccgg
     5701 tatccaggcc agcgtcaatg ctccgctcgg ccagggtttg ccgtccatcg tgatccgtgt
     5761 atcgggaata gagaactgcc agcccggatt ttcacgcgta atatgcgcgg tcagattatc
     5821 caccgacagc gtatgcgttt gcttctcgcc caaccagctg gcaccgccct gtttcagcca
     5881 gacgtcaccg ccggttacat cgcctttgtc gatcgtcatc cagccttcaa gggagaactg
     5941 tgccgtttcc agcgcaatat tgtcctgcat ccatttaccg agccacggct tcaggtcgat
     6001 gtcatccgcc tggagccaga cgcgaccatt gcttaacaac ccctcatcat cgcgcaaatc
     6061 catgcgcacc tgcatcacgc cgtgctgtcc ggtaaggctg gagaggctta ccaggccttc
     6121 cgcacggtgt cgacgtggat cgttcagcca ggtgagttgt gggatcgcca gctcggcgcg
     6181 ctgaccggat ggcgtcagga aactgacttc actgtcgcgg agatcgaaat ggtcaaattg
     6241 acgaagaaac agatcgctga tgtgactggc ttccagactg tcatcgctac caccgctggt
     6301 gataggagtg ttggtgcgaa agcgcagctg ccagaaagtg aggtcgcgaa actgccagcg
     6361 catatgtaac aggctctgcc agacatccag cgccagagta acgcgtttaa ccgaaaattc
     6421 gccgccatct tttagttctg cacggatgtc gtgtgcttca agcgtcgggc caaaattctg
     6481 ccagctggct gagagctgac tggcttcaac cggcatgcca gtcgcggatt ctattttgtt
     6541 gaggatttcc ggacgccagg cgtcaagatg cggtaaagca atacgcaggc cgctaaccag
     6601 cagggcagcg atcacaacga gcgcggctcc agtaagcagt aaaatccccg gcaatcgcct
     6661 cacccgtcac tccttgtctg ctaaatatgt gactcaaaaa ccctttgccg gatggcggcc
     6721 cagcatctgt ttacatcatt acgacgtcaa actgctcctg gttatagagc ggttcaattt
     6781 gtactttaac ctgtttacca acgaaaattt ccacttccgc cagcgagtgt gactcttcgc
     6841 ctttcaaggc ttcagctact gccggagaag catagaccag gaaacggtcg gagtcgtaag
     6901 catggtggac acgaacaatc tcgcgcatga tttcatagca taccgtttcc acggttttca
     6961 ccgttccgcg accgtggcag gttgggcatt cgttacacag tacgtgctca atgctttcgc
     7021 gggtgcgttt acgcgtcatc tccaccagcc ccagcgccga aaaaccatta acgctggttt
     7081 tcacccggtc tttgctcaac gcttgctcca gcgaatgcag cacgcggcgg cggtgatctt
     7141 cattattcat atcgatgaaa tcaataatga taatcccgcc cagattgcgc aaccgtaact
     7201 ggcgagcgat agcctgcgtc gcttcaatat tggtattgaa aatggtgtcg tccagattgc
     7261 gatgaccgac aaacgccccg gtattgatgt ccacggtggt catcgcttcg gtctggtcga
     7321 taatgagata accaccggat ttcagttcta ctttgcgttc cagcgctcgc tggatttcgt
     7381 tttcgacatc aaagagatcg aaaatcggct ggcgtccggt gtaatgctcc agcttgctgg
     7441 tcatctcggg aatgtactcc gaggtgaact caagtaatgc ttcgtaagtc aggcgtgagt
     7501 caacgcgaat gcggtccagt tcggcatcgg cgaaatcacg cagaacacgc tgcgccagcg
     7561 ccagttcgcc gtacagctga taacgggtct gcgggcgctt tttacgctcc ataactttgg
     7621 tccagacgcg tttcagataa gcggcatcgg aggccagttc agcctcgcca accccttccg
     7681 ctgcagtacg gatgataaac ccgccctgct cgtcgcaata ctccgcgacc acttttttca
     7741 ggcgttcacg ttctgattcg ctttcaatac gttgggaaac accaacgtga gaagcccctg
     7801 gcataaagac cagatagcga gaaggcagcg tgatatcggt ggtcaggcgc gcccccttag
     7861 tgccaagcgg atctttcacc acctgcacca tcagatcttg cccctgacga accagttccg
     7921 agatgtcgcg caccgtgaat tgcttttgtt cttcacccgc cacacattcg gtgtgcggca
     7981 tgatgtcaga tgcatgaaga aacgcggctt tatccagccc aatatctaca aaagccgcct
     8041 gcatacccgg aagtacacga cttacacgac ccttgtagat attgcctact attccgcgtc
     8101 gcgcctcacg ttcaatatga atttcctgca gaataccgcc atcaatatac gccactcgcg
     8161 tttccgaagg cgttacgttt actaacaatt cagccgtcat gtttatccct tttctcacgc
     8221 agtgcgttaa aattacttaa taattcatac gtttcaacca gcggtaagcc gactacggcg
     8281 tgatagctgc catttatctt cctgacaaaa cagccaccca gcccctgaat accgtatgca
     8341 cctgctttat ctaacggttc accgctggcg acatagcccg cgatgtcttc gtctgttaac
     8401 gttctgaaag tcacatcggt gaccaccagg caatcgagaa tgtgctggct gtcggccaac
     8461 gccactgccg tcatcacctg atgggtctga cccgataatt tgcgcaacat ctgcgccgca
     8521 tgctctgcgt cgcgcggttt ctccagcact tctccgttca ggataacgat agtatccgca
     8581 cccagtaccg ggagatcctt cgccgtttgc gcgacacctg cccgtgcttt ctcgcgcgcc
     8641 agacgcacaa catactgctg cgcgctctcc tgcggctgac gctgctcctc aatgcccgta
     8701 acaatacgtt caaaggtcac gccaagttgc gcaagtaact cctgacgacg cggagaaccg
     8761 gaagctaaat acagagaagt catagaaacc tttattgcac tgcaaactgc tgacggactt
     8821 tacgcatcag caagaaaatc cacggccaga gcaccccatt gactacacta ctccagaaca
     8881 cttccggtct gaaagagacg ttaatcacta aaaactctgc ccagaaaaca ataatatcca
     8941 ccaccagcga aagcaacatg acgaccagcg cctgctgcca taatgcgagg ttgcggaaaa
     9001 gctggtattt cagcgccacc aggtaagcaa tgatgctcat cgccaatacg cgtacgccaa
     9061 gcgtcgagcc gctgatcaga tccagtatgg cacccatcac aaaacctgtg cccacattta
     9121 cgcgatgggg caaggccagg atccaataca acaagatgag taacacccag tttggccgga
     9181 aaacaatcag gttgtccggc cagggcatga tttgcagcaa cagcgcaatg aggaaagaga
     9241 gccagattac ccagcgtccc tggctacgat agctcgccac tattgccctc ccggcgcacg
     9301 cgcaggcggt tgagcaccac tttgcggcgg cgtagccctt tgtggagagc ggttagcagc
     9361 aggctgtgtc ggcgcagcag gcgcagtagc tgcatttcct gtcgtgggtt gctgaggagt
     9421 cggctgagcg atccccgttg ccggttcagg taactttggc cccatcgcgt ctggcgaagg
     9481 caatacctgc ggcatcatct gcatcagacg ttcattagca acacgatgca cctcttccgg
     9541 cgtcatcggg ttagcgccgt tacgatctgc cccccacagc agcagcagat aacgcaaacg
     9601 ttgcagtcct gcagtcggac gcgcctgaat cacagtataa gcgcgctggg tatcgagttt
     9661 tacggaagag acaaccgcga ccggatagcc ttccgggaaa cgaccgccca gaccggaggt
     9721 caccagcaca tcaccaacac gaatatccgt attcgctggc agatgctcaa gctgcaaatc
     9781 atccgtacaa ccgttaccgg ctgcaattac gcggatatcg ttgcgcagca cctggattgg
     9841 cagcgcgtgg gtcgcatcac agatcagcag cacgcgactg gtcagtttag cgacggccac
     9901 cacctgacca acaacacctt tgtcgctgat gaccggctgg ccttcataaa cgccattaac
     9961 gctaccttta tcgataacaa cttgatcgct ataaggatcg ttaaccgtgg agataacctg
    10021 agtcaccatt ttctgctcat cctgacgcag cggggaaccc agcagctcgc gcagacgcgc
    10081 gttctcctgt ttgtattgtc caagcatcag cagttcactg tttttcagca acagttcctg
    10141 acgtaacgcc cggttttcaa gttctagttg gtcacgcgag gccagcgtct gcgatacgcc
    10201 atccagcaat tcacgaggag cattggaaac aaagtagaaa ggactgacgg cggtatccat
    10261 ataagtacgg atttgactga acgtccccag gcggctgtcg gcaataataa tgccgagcgc
    10321 caccagcacc gccagaataa ggcgaatctg tagcgacggg ccacggctaa aaattggctt
    10381 cataagttat gcgtattctc gtatcagacc aggcagggta aacagacgct tcccctgcct
    10441 gcatccgatt actcttcgct gaacaggtcg ccgccgtgca tgtcgatcat ttccagcgct
    10501 ttgccgccac cgcgcgccac acaggtcagc gggtcttcag caacaacgac tggaatgccg
    10561 gtttcttcca ttaacaaacg gtcaaggtta cgcagcagtg cgccaccacc ggtgagtacc
    10621 atgccgcgct cggagatgtc ggaagccagt tccggcgggc actgttccag tgcaaccatt
    10681 accgcgctca caataccggt cagcggttcc tgcagtgctt cgaggatttc gttggagttc
    10741 agggtaaaac cgcgtggaac accttctgcc aggttacggc cacgaacttc gatttcacgg
    10801 acttcatcgc ccggataagc cgaaccgatt tcgtgcttga tacgttctgc ggtggcttca
    10861 ccgatcagag aaccgtaatt acgacgcaca tagttgatga tagcttcgtc gaaacggtca
    10921 ccaccaatgc gtacggaaga ggagtaaacc acgccgttca aggagataac agcaacttca
    10981 gtggtaccac caccgatatc aaccaccata gaaccggtcg cttcagaaac cggcaggcca
    11041 gcaccaattg cggcagccat cggttcttca atcaggaaga cttcacgggc accagcgccc
    11101 tgcgcggatt cacgaattgc gcggcgttca acctgtgtcg cgccaaccgg tacacaaacc
    11161 agaacgcgcg ggcttggacg cataaagctg ttgctgtgca cttgtttgat gaagtgctgg
    11221 agcatttttt cagtcacgaa gaagtcggcg ataacgccgt ctttcattgg gcgaatggca
    11281 gcaatattgc ccggcgtacg gcccagcatc tgcttcgcgt catgacctac tgcagctacg
    11341 cttttcggtg aaccggcacg atcctgacga atggccacca cggaaggctc attcaatacg
    11401 atgccttgtc cttttacata aatgagggta ttcgcagtac ccaggtcaat ggacaagtca
    11461 ttggaaaaca tgccacgaaa ttttttcaac atactaaggg ataatcctga aagctggggc
    11521 ggaaaagaaa atccgcttac tttaccaacc acacgcagca gcgacaaggc gcaaaaatca
    11581 tctgctacgg tgaaaattag tgcagttcgt ttcctttgtt acaaatctct gcctgagtcc
    11641 agaaaggctt aatgcatcag cagcattcct cgcctgtttg caaccgcgta aggtcattca
    11701 tctgcatatg tgctgcaaca atctggcgag cagacaagca cactcccatg agacgcagcg
    11761 cgcgttattc tacgtgaaaa cggattaaac ggcaggttaa accgagtatc tttgtgaata
    11821 ttttttcacg ttagtatcaa gtggctgtga ggacgcgaaa aaatcccctt gcccgcctgt
    11881 aacaccgcgc tgaatcaggg tctgccactc gcttcgcgaa cgcacaccgg tggcgtaaac
    11941 ctgggtgctg gtccctgagc aggcttccac caggctttga accagcagct ggttctccgt
    12001 tcgcttctca atgtttctga ccagccccgg atggagcttg agtaactcaa cattaagttc
    12061 tttgatccaa ctggtgctta ccagcgtcaa accagcctgg ttgacggcta cccgtacccc
    12121 taaagcattc actaaacgaa taacaggttg taaacgactg atatgttgac ctacatcggc
    12181 ctctgcaagt tcaataatta tgcgttttcg ttgtgatttt tcacattgca ttaacgtatc
    12241 gcgcagccaa cgctgaaaac gcgggcgaat cagcgactca acggtaacct gaatcgccag
    12301 attttcctct ggccagtaac gcaatagtgg aataagacgg ctgatttgca gacggtcata
    12361 ctcttccgat aagccaaact gcaagaccat cggcatatac tctgccgagc taacctcttc
    12421 attaccatca aagatgcggc acatgagttc gcgatgatga acctgacctt cgcgagtaac
    12481 cgccggtttt tgataaaggc gcgggccgcc gcgactgagc atttgctcga taagcgtacg
    12541 ccagcgaaca ttaccgcgtc ctttttcagg caacgagtca tcgtaaatag cccagctatt
    12601 gccgccctgc aatcccgcat tacgcgtggc agactctgca tgttccatta cctgctcggt
    12661 atcctgacca ctacgccagg cgcagatacc aatgtggatc atatcgtcgc gatcgagcat
    12721 tttattgttc ggcaaggtat cgacggcttt gattaactga ccggcgatgc tctctgcctc
    12781 ttttaacgtc cggtgcggta acagcgcagc aaaatcactg cggtggtaac gcgccagcag
    12841 tgcgccaggg tagcgcatca taaatgtcga cagcagattc gtcagagtga agaactgttc
    12901 ttcaacctgg ctgtgccccc aggtatcgct caacatattg aaatccggca gacgaatcat
    12961 catcacgatc ccgtgggtac ctactttctc ctgatcttcc agtaacgttg ctaactgatt
    13021 atcgaaaaag agtcggttat tgaggccggt tttcacgtcc tgtgcggcat aagagcggat
    13081 cagcgtatca agacggctgt gttgttcgcg tgcgttctga atttcacgaa gcagcgtatc
    13141 cagcgcactg ctggttgtgg gcggccattc atagatggtt cccaacacat tagagccacg
    13201 ctcaccgttt aagatacgag tagcccgggt ttccagcaat tcttgcccgg caagttgccg
    13261 ttgtaaccag cgtaccgcca gaaagagcat aagaatgata aagccaatcg cccccgtgag
    13321 cggcgcggtg gtcatcaacg aatggaaata gttgcccatc ggatcctgat aaaccagacg
    13381 caacgacatc cccggatgct ttatcaacgg aacgctcagt tcacgaaaca gatcgctggt
    13441 gccaaccgga cgataactac catttctggc cagggtataa acctgtttat caccatgcag
    13501 caggtctaca cgaacgatat ctgctgacac cattaattcg gtaatttgtg gccttaatgc
    13561 gctgaagtca ttcgacacaa ggtgggtatc aatcgccgtc gccaccgcct gaacgcgatg
    13621 actaaactta tactgaatgg cgttgtagaa acttagcgaa cagcccagca aagtcacaaa
    13681 aattgttaac ccggtgagca gcgtaacaaa ggccgaaaat ttcgtcgtta atctcatcct
    13741 tgtgttaact ccgatagtga ggaagcgggc atactagcaa atcagattta tctcgcaatt
    13801 tattgcgcgt catcggcttt gcttttccat tagcgagtat agtcttcaga aattattttc
    13861 caatccatca tgcacatgag gaccacttat gcaggcgtta cttttagaac agcaggacgg
    13921 caaaactctc gcatcagtac agactctgga cgaaagtcgc ctgccggagg gcgatgtcac
    13981 ggtcgatgtt cactggtcga gcctgaacta taaagatgcg ctggcgatta ccggtaaggg
    14041 aaaaatcatc cgtaattttc cgatgattcc tgggatcgat tttgccggaa ctgtacgcac
    14101 cagcgaagat ccgcgttttc atgccggtca ggaggtgtta ctcactggct ggggcgttgg
    14161 tgaaaaccac tggggtgggc tggcggagca ggcgcgagtg aaaggtgact ggctggttgc
    14221 catgccgcaa gggctggacg cgcgtaaagc aatgattatc ggtactgccg gttttaccgc
    14281 catgctgtgt gtgatggcgc tggaagatgc cggtgttcgc ccgcaggacg gggagattgt
    14341 cgtgacgggt gccagtggtg gcgtcggcag taccgccgtg gcgctgctgc ataagttggg
    14401 ttatcaggtc gttgccgttt ccggtcgcga aagtacccat gaatatctga aaagtttagg
    14461 tgccagccgt attctccctc gtgatgagtt tgccgaatcc cgtcctctgg aaaaacaagt
    14521 ctgggctggg gcaattgaca ctgttggcga caaagtgctg gcaaaagtgc tggcgcaaat
    14581 gaattacggc ggctgcgtgg cggcctgtgg tctggcgggt ggttttactc tgccaaccac
    14641 ggtcatgcca tttattctgc gtaatgtccg tttgcaaggg gtggattcag taatgacgcc
    14701 accagaacgc cgcgcacaag cctggcagcg actggtcgcc gatttaccgg aatcattcta
    14761 tacccaggcg gcaaaagaga tatctctgtc agaggcaccg aactttgccg aggccatcat
    14821 taataaccag atccaaggtc gcacgctggt gaaggttaac taaccattta gcaaggaata
    14881 ataagagagg gaactcattt tgaaatcatt attcaatcgg ttaacgggaa aagcggttag
    14941 ccggacagct ttcgtagaac accttggtca tgaagttgta caacatcatc caaactggaa
    15001 agtcatgatt tcgactgacc ataaattgat gcgcattgat actccactaa acagctatta
    15061 ttgatacgcc tccgtcgcct gttaggttta tggtgctttg cctgggcgac gctacgctta
    15121 accccttact tatttctggt accatggggt gaataatctg attttgtttg actacaaatt
    15181 aatcactcga acctatttaa tgctgagcat tgtcaatcgg ttaattttgc gtgctttagc
    15241 attcacatct atccagacga tgcagtgaaa attgggtaat ccccagcaac cgctgcgtaa
    15301 tgtcgtctat cttgtcgcga tcctggcatc cctacattat ttgtggtctg tgaagattat
    15361 ctcattgcag cccctcatct tcgcagggct ggctttacag cttttcacct tacgttataa
    15421 gaagttccgt cgatgatggc gctaatttcg tgaattgtgc ggcttgttgc aaattacacg
    15481 gtattgaagg ttatttacat gttagctgtt gattatcttc cctgataaga ccagtattta
    15541 gctgccaatt gctacgaaat cgttataatg tgcgacctcg tcctccctga cgcagttttt
    15601 gcgctgcgga aaaggtgaca ttggcgcaac gaaggtatat tttgtttttt gccggaggat
    15661 agcagcagat cgctgcacaa tgtccgtcaa gtctaacatt gacactctgg ggcaaaatag
    15721 accggcgtcc cggcctgctg gaatttatcg ctatgcatac agctgtcggg gcatacgctt
    15781 tacagacggc ggtgaaacgc ctgtcacaat cacactaaac aaagagtacg gaacccactc
    15841 atggatattc gtaagattaa aaaactgatc gagctggttg aagaatcagg catctccgaa
    15901 ctggaaattt ctgaaggcga agagtcagta cgcattagcc gtgcagctcc tgccgcaagt
    15961 ttccctgtga tgcaacaagc ttacgctgca ccaatgatgc agcagccagc tcaatctaac
    16021 gcagccgctc cggcgaccgt tccttccatg gaagcgccag cagcagcgga aatcagtggt
    16081 cacatcgtac gttccccgat ggttggtact ttctaccgca ccccaagccc ggacgcaaaa
    16141 gcgttcatcg aagtgggtca gaaagtcaac gtgggcgata ccctgtgcat cgttgaagcc
    16201 atgaaaatga tgaaccagat cgaagcggac aaatccggta ccgtgaaagc aattctggtc
    16261 gaaagtggac aaccggtaga atttgacgag ccgctggtcg tcatcgagta acgaggcgaa
    16321 catgctggat aaaattgtta ttgccaaccg cggcgagatt gcattgcgta ttcttcgtgc
    16381 ctgtaaagaa ctgggcatca agactgtcgc tgtgcactcc agcgcggatc gcgatctaaa
    16441 acacgtatta ctggcagatg aaacggtctg tattggccct gcaccgtcag taaaaagtta
    16501 tctgaacatc ccggcaatca tcagcgccgc tgaaatcacc ggcgcagtag caatccatcc
    16561 gggttacggc ttcctctccg agaacgccaa ctttgccgag caggttgaac gctccggctt
    16621 tatcttcatt ggcccgaaag cagaaaccat tcgcctgatg ggcgacaaag tatccgcaat
    16681 cgcggcgatg aaaaaagcgg gcgtcccttg cgtaccgggt tctgacggcc cgctgggcga
    16741 cgatatggat aaaaaccgtg ccattgctaa acgcattggt tatccggtga ttatcaaagc
    16801 ctccggcggc ggcggtggtc gcggtatgcg cgtagtgcgc ggcgacgctg aactggcaca
    16861 atccatctcc atgacccgtg cggaagcgaa agctgctttc agcaacgata tggtttacat
    16921 ggagaaatac ctggaaaatc ctcgccacgt cgagattcag gtactggctg acggtcaggg
    16981 caacgctatc tatctggcgg aacgtgactg ctccatgcag cgccgccacc agaaagtggt
    17041 cgaagaagca ccagcaccgg gcattacccc ggaactgcgt cgctacatcg gcgaacgttg
    17101 cgctaaagcg tgtgttgata tcggctatcg cggtgcaggt actttcgagt tcctgttcga
    17161 aaacggcgag ttctatttca tcgaaatgaa cacccgtatt caggtagaac acccggttac
    17221 agaaatgatc accggcgttg acctgatcaa agaacagctg cgtatcgctg ccggtcaacc
    17281 gctgtcgatc aagcaagaag aagttcacgt tcgcggccat gcggtggaat gtcgtatcaa
    17341 cgccgaagat ccgaacacct tcctgccaag tccgggcaaa atcacccgtt tccacgcacc
    17401 tggcggtttt ggcgtacgtt gggagtctca tatctacgcg ggctacaccg taccgccgta
    17461 ctatgactca atgatcggta agctgatttg ctacggtgaa aaccgtgacg tggcgattgc
    17521 ccgcatgaaa aatgcgctgc aggagctgat catcgacggt atcaaaacca acgttgatct
    17581 gcagatccgc atcatgaatg acgagaactt ccagcatggt ggcactaaca tccactatct
    17641 ggagaaaaaa ctcggtcttc aggaaaaata agactgcaaa agcgtcaaaa ggccggattt
    17701 tccggccttt tttattactg gggatcgaca acccccataa ggtacaatcc tcgctttctt
    17761 cacccatcag ggacaaaaaa tggacactcg ttttgttcag gcccataaag aggcgcgctg
    17821 ggcgctgggg ctgacccttt tgtatctggc agtttggtta gcagccgctt acttacctgg
    17881 cgttgccccc ggttttaccg gctttccgcg ctggtttgag atggcctgca tcctgacgcc
    17941 gctgctgttt attggactgt gctgggcgat ggtgaaattt atctatcgcg atatcccgct
    18001 ggaggatgac gatgcagctt gaagtaattc taccgctggt cgcctatctg gtggtggtgt
    18061 tcggtatctc ggtttatgcg atgcgtaaac ggagcaccgg caccttcctt aatgagtatt
    18121 tcctcggcag ccgctctatg ggcggtattg tgctggcgat gacgctcacc gcgacttata
    18181 tcagtgccag ttcgtttatc ggcgggcctg gagctgctta caaatacggc cttggctggg
    18241 tattgctggc gatgattcag cttcctgcag tctggctttc actcggtatt ctcggcaaga
    18301 agtttgcgat tcttgcgcgc cgctacaatg cagtgacgct gaacgatatg ctgtttgccc
    18361 gctatcagag tcgtcttttg gtgtggctgg cgagtttgag tttgctggtc gcgtttattg
    18421 gcgcgatgac cgtgcagttt atcggcggcg cgcgcctgct ggaaaccgcg gcgggtattc
    18481 cttatgaaac cgggctgctg atttttggta tcagcattgc gttatatacc gcatttggtg
    18541 gctttcgcgc cagcgtgctg aacgacacca tgcaagggct tgtgatgctg attggcaccg
    18601 ttgtgctgct tattggcgtg gtacatgccg ctggcggctt aagcaacgca gtacagacct
    18661 tgcaaaccat cgatccgcaa ctggttacgc cacaaggcgc tgacgatatt ctgtcgcctg
    18721 cctttatgac gtcgttctgg gtactggtgt gctttggcgt tataggcctg ccgcatactg
    18781 cggtgcgctg tatctcttat aaagacagca aagcagtaca ccgggggatc atcatcggta
    18841 cgattgtagt cgcaatcctg atgttcggta tgcacctggc cggagcgtta ggtcgtgcag
    18901 tgatccccga tctcaccgta ccggatctgg tgatcccgac gttaatggta aaagtgctgc
    18961 caccgtttgc tgccgggatc tttctggctg cacctatggc tgcgatcatg tcgacaatta
    19021 acgcccaatt gctacaaagt tccgctacga tcattaaaga tctctatctg aatatccgtc
    19081 cggatcaaat gcaaaacgag acgcgtctga agcggatgtc ggcggtaatt acgttagttc
    19141 tcggcgcgtt gctgctgctt gccgcctgga agccgccaga aatgatcatc tggctgaatt
    19201 tgctggcctt cggtgggctg gaagccgttt tcctgtggcc gctggtgctg ggtctttact
    19261 gggaacgcgc caacgccaaa ggcgcgctaa gtgcgatgat cgttggcggc gtgctgtatg
    19321 ccgtactcgc gacgctgaat attcagtacc tgggcttcca ccctatcgtg ccctcgttac
    19381 tactaagttt gctggctttc ctggtcggaa accgtttcgg tacatccgtc ccgcaagcta
    19441 ccgttttgac tactgataaa taaagagttt tgccatgcct tggatccaac tgaaactgaa
    19501 caccaccggc gcgaacgcgg aagatcttag cgatgcgctg atggaagcgg gtgccgtttc
    19561 tatcactttt caggacaccc acgatacgcc agtatttgag ccgctgccgg gcgaaacgcg
    19621 cctgtggggc gacaccgatg tgattggtct gttcgacgct gaaaccgata tgaccgacgt
    19681 ggtggcgatt ctggaaaacc atccgctgct cggcgcaggc ttcgcgcata aaatcgaaca
    19741 actggaagat aaagactggg agcgcgaatg gatggataat ttccacccga tgcgctttgg
    19801 tgaacgactg tggatctgcc caagctggcg tgatgtgccg gacgaaaacg ccgtcaacgt
    19861 gatgttagat ccagggctgg cgtttggtac gggtacccat ccaaccacct ctctgtgcct
    19921 gcaatggctc gacagcctcg atttaaccgg taaaacagtc atcgactttg gctgtggttc
    19981 cggtattctg gcgatcgcgg cgctgaaact gggtgcagca aaagccattg gtattgatat
    20041 cgatccgcag gcgattcagg ccagccgcga taacgccgaa cgtaatggcg tttctgaccg
    20101 tctggaactc tacttaccga aagatcagcc agaagaaatg aaagccgacg tggtggtcgc
    20161 taacatcctt gcaggcccat tacgtgaact ggcaccgtta atcagcgtcc tgccggtttc
    20221 aggcggtttg ctgggccttt ccggtattct ggcaagccag gcagagagcg tttgtgaagc
    20281 ttatgccgac agcttcgcac tggacccggt cgtggaaaaa gaagagtggt gccgtattac
    20341 cggtcgtaag aattaacctt cgcatcgccg tagggtgacg cggggcgaag tgcgagcaag
    20401 ctcacaaaag gcacgtaaat ttgccgatta tttacgcaaa tttgcgtgcc aaaattttca
    20461 ttcataaaga aaaattgaga acttactcaa atttctttga gtgtaaattt tagtcactat
    20521 tttctaatat gatgattttt atgagtaatt atcgcaccac gctcatttta aatgcaattc
    20581 tttgatccat ctcagaggat tggtcaaagt ttggcctttc atctcgtgca aaaaatgcgt
    20641 aatatacgcc gccttgcagt cacagtatgg tcatttctta actcatgcgc atcggacaat
    20701 atcagctcag aaatcgcctg atcgcagcgc ccatggctgg cattacagac agaccttttc
    20761 ggacgttgtg ctacgagatg ggagccggat tgacagtatc cgagatgatg tcttctaacc
    20821 cacaggtttg ggaaagcgac aaatctcgtt tacggatggt gcacattgat gaacccggta
    20881 ttcgcaccgt gcaaattgct ggtagcgatc cgaaagaaat ggcagatgca gcacgtatta
    20941 acgtggaaag cggtgcccag attattgata tcaatatggg ttgcccggct aaaaaagtga
    21001 atcgcaagct cgcaggttca gccctcttgc agtacccgga tgtcgttaaa tcgatcctta
    21061 ccgaggtcgt caatgcagtg gacgttcctg ttaccctgaa gattcgcacc ggctgggcgc
    21121 cggaacaccg taactgcgaa gagattgccc aactggctga agactgtggc attcaggctc
    21181 tgaccattca tggccgtaca cgcgcctgtt tgttcaatgg agaagctgag tacgacagta
    21241 ttcgggcagt taagcagaaa gtttccattc cggttatcgc gaatggcgac attactgacc
    21301 cgcttaaagc cagagctgtg ctcgactata caggggcgga tgccctgatg ataggccgcg
    21361 cagctcaggg aagaccctgg atctttcggg aaatccagca ttatctggac actggggagt
    21421 tgctgccccc gctgcctttg gcagaggtta agcgcttgct ttgcgcgcac gttcgggaac
    21481 tgcatgactt ttatggtccg gcaaaagggt accgaattgc acgtaaacac gtttcctggt
    21541 atctccagga acacgctcca aatgaccagt ttcggcgcac attcaacgcc attgaggatg
    21601 ccagcgaaca gctggaggcg ttggaggcat acttcgaaaa ttttgcgtaa acagaaataa
    21661 agagctgaca gaactatgtt cgaacaacgc gtaaattctg acgtactgac cgtttctacc
    21721 gttaactctc aggatcaggt aacccaaaaa cccctgcgtg actcggttaa acaggcactg
    21781 aagaactatt ttgctcaact gaatggtcag gatgtgaatg acctctatga gctggtactg
    21841 gctgaagtag aacagcccct gttggacatg gtgatgcaat acacccgtgg taaccagacc
    21901 cgtgctgcac tgatgatggg catcaaccgt ggtacgctgc gtaaaaaatt gaaaaaatac
    21961 ggcatgaact aattcaggtt agctaaatgc ttgattaaaa aggcgctact cggcatgggg
    22021 aagcgccttt tttataggtg tcacaaaggg agtgaccatg agaacaggat gtgaaccgac
    22081 ccggtttggt aatgaagcta agaccattat tcacggtgat gcccttgccg aacttaaaaa
    22141 gctatctact gaaagcgtcg atctgatctt tgccgaccca ccgtataaca tcggtaaaaa
    22201 ttttgatggt ctgatcgaag cctggaaaga agatctgttt atcgactggc tgtttgaagt
    22261 gattgcagag tgccaccgcg ttctgaaaaa gcagggcagc atgtacatca tgaacagcac
    22321 ggaaaacatg ccctttatcg atctccagtg ccgcaagctt tttaccatca aaagtcgcat
    22381 cgtctggtca tatgacagtt ctggagtgca ggcgaaaaaa cactacggtt ccatgtacga
    22441 acccatcctg atgatggtga aagacgcaaa gaactacacg ttcaacggtg atgctattct
    22501 ggtagaagcc aaaactggat cgcagcgcgc gttgatcgat tatcgcaaaa atcctccaca
    22561 gccttacaat catcaaaaag taccgggtaa cgtctgggat tttccgcgcg tgcgttattt
    22621 gatggatgaa tatgagaacc acccgacgca aaaaccggaa gccttactga aacgcattat
    22681 tcttgcctct tccaacccag gcgatatcgt tctcgacccg tttgccggta gctttactac
    22741 cggtgccgta gccgtctcca gcgggcgaaa attcattggt attgagatca acagcgagta
    22801 catcaaaatg gggcttcgac ggctggatgt cgcgtcgcat tactctgcgg aagaactggc
    22861 gaaagtgaaa aaaagaaaga ccggcaacct gtcaaaacga agtcggttaa gcgaagttga
    22921 ccccgatctc attgcaaagt aaaggaagtg taagcctggt tttcagatta ttcatttcgt
    22981 gtatatttcc tgccagactt ggttaaacat gcacaggctc aggtaatgat tcgcaagtat
    23041 tggtggctcg tcgttttcgc tgtcttcgtt tttctgtttg atactttact gatgcagtgg
    23101 attgaactgc tggcaacaga aacagacaaa tgccgcaata tgaactcagt taatccacta
    23161 aaactggtta actgtgacga actgaatttt caggacagaa tgtgaattta catgacactt
    23221 aattcattcg tttgatgaat taatttcgtt atgttttcat ctggcatgaa cattcttaat
    23281 acgttatcga ccagagcggg ggcttgttta taaagatcat aacccgccat attcattaac
    23341 cagttttgaa caattccgct gaaggcacca tcaataataa tcatcacaac atctaaatcg
    23401 aggttatttg ctacacaacc ttgttgctga cacgcctgca atacttcgcg gagagtctgc
    23461 ggattaaagc ccatcttttc gcgtatcact ccctcggcca gcatctcatc attaaattca
    23521 catttgtgat ataagatttt cagcaacgcc tgctggcggg gaattttggc aatatattgc
    23581 aagccgacaa tcaatttttc acgcaattgt tgaaacgggt catgctctaa tccagccgtc
    23641 aagtgttcct ggattaactc ccgcaatgaa ggctgttgca accacatctc attaaacagt
    23701 tgagtcttgt tttcgaagtg ccagtagata gcgccacgcg taacgttagc ggcgtcggca
    23761 atgtcgttga gcgtcgtctt gcttacgcca tgctgcgcaa actgggcgat ggcagtttca
    23821 atcagttctt gccgggtctt cagagcttcg gctttggttc tttttgccat gattaattat
    23881 tcaggaaata aatatattcg acacagagtg agaaaatagc gaaggttaat ctatcaccta
    23941 atgtgtattt a
//
LOCUS       DAZKFU020000049        23749 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZKFU020000049 DAZKFU020000000
VERSION     DAZKFU020000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.049"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(155..1066)
                     /gene="gsiD"
                     /locus_tag="ACHOQQ_RS07785"
                     /old_locus_tag="pgaptmp_001557"
     CDS             complement(155..1066)
                     /gene="gsiD"
                     /locus_tag="ACHOQQ_RS07785"
                     /old_locus_tag="pgaptmp_001557"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGU4469825.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIMVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1069..1989)
                     /gene="gsiC"
                     /locus_tag="ACHOQQ_RS07790"
                     /old_locus_tag="pgaptmp_001558"
     CDS             complement(1069..1989)
                     /gene="gsiC"
                     /locus_tag="ACHOQQ_RS07790"
                     /old_locus_tag="pgaptmp_001558"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGU4469826.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2007..3545)
                     /gene="gsiB"
                     /locus_tag="ACHOQQ_RS07795"
                     /old_locus_tag="pgaptmp_001559"
     CDS             complement(2007..3545)
                     /gene="gsiB"
                     /locus_tag="ACHOQQ_RS07795"
                     /old_locus_tag="pgaptmp_001559"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGU4469827.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFNTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3565..5436)
                     /gene="gsiA"
                     /locus_tag="ACHOQQ_RS07800"
                     /old_locus_tag="pgaptmp_001560"
     CDS             complement(3565..5436)
                     /gene="gsiA"
                     /locus_tag="ACHOQQ_RS07800"
                     /old_locus_tag="pgaptmp_001560"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGU4469828.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQVLRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAPVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5423..6388)
                     /gene="iaaA"
                     /locus_tag="ACHOQQ_RS07805"
                     /old_locus_tag="pgaptmp_001561"
     CDS             complement(5423..6388)
                     /gene="iaaA"
                     /locus_tag="ACHOQQ_RS07805"
                     /old_locus_tag="pgaptmp_001561"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4469829.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSSHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDAVATQ"
     gene            6592..7827
                     /gene="moeA"
                     /locus_tag="ACHOQQ_RS07810"
                     /old_locus_tag="pgaptmp_001562"
     CDS             6592..7827
                     /gene="moeA"
                     /locus_tag="ACHOQQ_RS07810"
                     /old_locus_tag="pgaptmp_001562"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGU4469830.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7827..8576
                     /gene="moeB"
                     /locus_tag="ACHOQQ_RS07815"
                     /old_locus_tag="pgaptmp_001563"
     CDS             7827..8576
                     /gene="moeB"
                     /locus_tag="ACHOQQ_RS07815"
                     /old_locus_tag="pgaptmp_001563"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGU4469831.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8652..9314)
                     /gene="fsa"
                     /locus_tag="ACHOQQ_RS07820"
                     /old_locus_tag="pgaptmp_001564"
     CDS             complement(8652..9314)
                     /gene="fsa"
                     /locus_tag="ACHOQQ_RS07820"
                     /old_locus_tag="pgaptmp_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4469832.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMAITAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            9445..10344
                     /gene="ybiY"
                     /locus_tag="ACHOQQ_RS07825"
                     /old_locus_tag="pgaptmp_001565"
     CDS             9445..10344
                     /gene="ybiY"
                     /locus_tag="ACHOQQ_RS07825"
                     /old_locus_tag="pgaptmp_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGU4469833.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10350..12782
                     /gene="ybiW"
                     /locus_tag="ACHOQQ_RS07830"
                     /old_locus_tag="pgaptmp_001566"
     CDS             10350..12782
                     /gene="ybiW"
                     /locus_tag="ACHOQQ_RS07830"
                     /old_locus_tag="pgaptmp_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGU4469834.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRFHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12928..13743
                     /gene="ybiV"
                     /locus_tag="ACHOQQ_RS07835"
                     /old_locus_tag="pgaptmp_001567"
     CDS             12928..13743
                     /gene="ybiV"
                     /locus_tag="ACHOQQ_RS07835"
                     /old_locus_tag="pgaptmp_001567"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGU4469835.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13890..15155
                     /gene="ybiU"
                     /locus_tag="ACHOQQ_RS07840"
                     /old_locus_tag="pgaptmp_001568"
     CDS             13890..15155
                     /gene="ybiU"
                     /locus_tag="ACHOQQ_RS07840"
                     /old_locus_tag="pgaptmp_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGU4469836.1"
                     /translation="MAPTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAG
                     DSVWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15396..16988)
                     /gene="ybiT"
                     /locus_tag="ACHOQQ_RS07845"
                     /old_locus_tag="pgaptmp_001569"
     CDS             complement(15396..16988)
                     /gene="ybiT"
                     /locus_tag="ACHOQQ_RS07845"
                     /old_locus_tag="pgaptmp_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGU4469837.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17207..18127
                     /gene="ldtB"
                     /locus_tag="ACHOQQ_RS07850"
                     /old_locus_tag="pgaptmp_001570"
     CDS             17207..18127
                     /gene="ldtB"
                     /locus_tag="ACHOQQ_RS07850"
                     /old_locus_tag="pgaptmp_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4469838.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18185..19303)
                     /gene="ybiR"
                     /locus_tag="ACHOQQ_RS07855"
                     /old_locus_tag="pgaptmp_001571"
     CDS             complement(18185..19303)
                     /gene="ybiR"
                     /locus_tag="ACHOQQ_RS07855"
                     /old_locus_tag="pgaptmp_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGU4469839.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAVGFALLARRVVLSVDWTLLLVFMAMFIDVHLLIQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19300..19767)
                     /gene="mntR"
                     /locus_tag="ACHOQQ_RS07860"
                     /old_locus_tag="pgaptmp_001572"
     CDS             complement(19300..19767)
                     /gene="mntR"
                     /locus_tag="ACHOQQ_RS07860"
                     /old_locus_tag="pgaptmp_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGU4469840.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            19953..20081
                     /gene="mntS"
                     /locus_tag="ACHOQQ_RS07865"
                     /old_locus_tag="pgaptmp_001573"
     CDS             19953..20081
                     /gene="mntS"
                     /locus_tag="ACHOQQ_RS07865"
                     /old_locus_tag="pgaptmp_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGU4469841.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20353..21936
                     /gene="opgE"
                     /locus_tag="ACHOQQ_RS07870"
                     /old_locus_tag="pgaptmp_001574"
     CDS             20353..21936
                     /gene="opgE"
                     /locus_tag="ACHOQQ_RS07870"
                     /old_locus_tag="pgaptmp_001574"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4469842.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKDGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(21986..22501)
                     /gene="ompX"
                     /locus_tag="ACHOQQ_RS07875"
                     /old_locus_tag="pgaptmp_001575"
     CDS             complement(21986..22501)
                     /gene="ompX"
                     /locus_tag="ACHOQQ_RS07875"
                     /old_locus_tag="pgaptmp_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGU4469843.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22554..22619)
                     /locus_tag="ACHOQQ_RS07880"
                     /old_locus_tag="pgaptmp_001576"
     CDS             complement(22554..22619)
                     /locus_tag="ACHOQQ_RS07880"
                     /old_locus_tag="pgaptmp_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469844.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            22854..23741
                     /gene="rhtA"
                     /locus_tag="ACHOQQ_RS07885"
                     /old_locus_tag="pgaptmp_001577"
     CDS             22854..23741
                     /gene="rhtA"
                     /locus_tag="ACHOQQ_RS07885"
                     /old_locus_tag="pgaptmp_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGU4469845.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         5782 a         6492 c         5914 g         5561 t
ORIGIN      
        1 ctgtctgaac gctgtgccgc tgcaaaaaca gtcccttgct gcggctttta accaaattac
       61 ccgattctac aaaatcagca gaaagttaca acacttgctt agggcaataa tttcctgtga
      121 ggtgattact ctttcaagca atattcaaac gtaactatcc tttaattttc ggatccagcg
      181 catcgcgtaa accatcgccc aacaaattga acgccagtac agtcagaaaa atagccaggg
      241 ccggaaaaac agcgacatgc ggcgcgataa ccatatccgc tcgagcctca ttgagcattg
      301 ctccccactc tggtgtcggc ggctgcgcac cgaggccgag aaatgacagg ctggcggcag
      361 agataatcga agtgccaatg cgcatggtga aaaacaccac gatagacgag acggtcccag
      421 gcaagatatg acgcaataaa atggtcatat cgctggcacc aatactgcgt gctgactcaa
      481 taaaggtttg ctgtttcaac accagcgtgt tgccgcgcac caggcgggca aacgcgggga
      541 tggaaaaaat ggcgactgca ataatcacgt tagcaatgcc gcttcccaac accgcaacaa
      601 cagcgatcgc cagtaaaata cccgggaagg caaacagcac atcgcaaatg cgcatgatca
      661 gccgatccca ccagccttca taatatccag ccagcaagcc cagcaacgtc cctatcgccg
      721 caccgataaa cacggcaaac acgcccgcag ccagcgagat ttgcgcacca accaggacac
      781 ggctgaaaat gtcgcgcccc agtgaatcga cgccaaacca gtgctgcaaa gaaggtccgt
      841 tattcagatt gtcataatca aaataatttt cggcgtcata gggagcgatc cagcgtgcaa
      901 aaatggccac cataatcaat aaaataacga ataatgcggc ggtcatcgcc atatgctgac
      961 ggcgaaatcg tcgccagaat tcatgccacg gtgtacgtac ctggtcaggt ttgaccagtg
     1021 gcatggcgtt taacaccgcc tgacgtcgcc agttaaatag tcgcatcctt acttgtacct
     1081 gatagccggg ttaatggcgg cgtaaagcac atccaccact aagttgataa gaataaattc
     1141 cagcgagaaa agcagaattt ccgcctgaat caccgggtaa tcgcgcattt ctacggagtc
     1201 aacgagtaag cgtccaagtc cgggccagtt gaaaactttc tccaccacga tagaaccacc
     1261 gagcaaaaag ccaaactgta agcccatcat ggtcactacc gggatcatcg cgttacgtag
     1321 cccgtgtttg aggacaaccc aggtttcgct cacccctttc gccctcgcgg tacgcatata
     1381 atcttcgctt aaaacgtcga caaacgacgc gcgggtaaag cgcgccatca cggcggccac
     1441 tgccgcgccg agcgtcaggg agggtaaaat gtagtgctgc caactgtctg ctcccacggt
     1501 aggcagccag cccagttcaa cggagaatac ctgaattaaa agcatcccca gagcaaatgc
     1561 cggaaacgag atccccgaca ccgcaatggt catactcaat cgatccggcc aacgattacg
     1621 ccagacggcg gcgataattc ccgccgccat accaaatatc accgcccaga ccatactggt
     1681 tatggtcagc cacagcgttg gcataaagcg gctggcaatc tcatcggcaa ccggacgacg
     1741 cgacaccatc gacaggccaa aatccccctg cacagcattg ctgatatagt gccagaactg
     1801 gtgatacagc ggctgatcca accccagctg ctgacgcacc agttctataa cctgcgcatc
     1861 agcttcgggc ccggcaatca atcgcgccgg atcgccgggc agcatatgca caaataaaaa
     1921 caccagcacc gagacgataa acagcgtcgg aatcaacccc agtaatcgtt tgataacgta
     1981 attaagcatt ccactccctg cgttgcttat tgcaaatccg cgtcttcaaa gctgaagccg
     2041 gtgtctggca tgatccaaaa accggtcagg tttttactgt gtgccgacac cagtttttct
     2101 accaccagcg ggatccacgg cgactcttgc cagatgatat cctgcgccgc cttatacaag
     2161 cgggtctttt ccgccggatc attagttttc agtgcctgag ccaggaagtc atccacctgt
     2221 ttattgctgt aaaacgcggt attaaacagc gtcggtggcc agttctgcga ggcaaacagc
     2281 ggcgatagtg cccagtccgc ttcgccggtt gaagccgacc agccagtgta gaacatccgc
     2341 acgccgctct ctttttgccc tttaccttca acttctgccg cccgctgtcc ggcatccatc
     2401 gcagtcacct gggctttaat cccgacctgc gctaactgct gctgggtaaa ttgcagcact
     2461 ttctgcgcgg tgctgtggtt atgtgacgac cacagcgtgg tattgaaacc attgggatat
     2521 cccgcctctt tcagtaattc gcgcgctttc actggatcgt aaggccacgg tttataactt
     2581 tgcgcgtagg cgatactcgg cggtaccaca ccagtagctg gcgttgcata gcccgcgaag
     2641 gccactttca ccagcgccgg acggttaatg gcgtaattca gcgcctcacg gaccttcggg
     2701 ttatcgaatg gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg
     2761 gccatcaact cgatattttt gtttttctcc agcagtgcgg cctgctcgta aggaatgggg
     2821 aaagcaaact gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc
     2881 ggacgccagg ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt
     2941 ttcaccttca caaaatcagt ctgattccag gtatccagtt catacggtcc ggttcccacc
     3001 ggatgaaaac caatctcctt gccatatttt tccagcgctg ccggtgaaat catcgcggtc
     3061 gccggatggg caagaatatt aataaacgct gagaacggct gtttgagggt aatctttacc
     3121 gttgtcggat cgatcgcttc cgttttagca atattcttat acaggttata gcgtttaaga
     3181 tgatttgccg gatcgctggc ccggtccaga ttcgctttca ccgctgcggc gttgaaatcg
     3241 gtgccatcct ggaatttaat gccttcccgc aatttcacgg tgtaagtaat gccgtcatcg
     3301 gaaacggtat aactctccgc cagcacgttt ttcagtttca tctctttatc cagaccgaac
     3361 agcccctggt aaaacgattt cgctacggcc tgagataacg tgtcattcgc gtcatacgga
     3421 tcgagcgtgg tgaaattcga tcctaccgcc accaccacat ctttggcagc gaatgcacaa
     3481 gatgccatca acgctgtcgc aatgcccagc gccactaacc cactacggtg tacagctctt
     3541 gccattttat tctccgcctg aatgttatct acgcatgaat gcgtattctg attgtggttg
     3601 tgcgacgtaa tgccccggcc cgacgcattg caacgagacg ggtgcaacct cttcgccacg
     3661 cagatgaata ttgctgggaa gatcgtccga cagcagtaca cgctgcggtc gttgtcggga
     3721 cggttcagcg accggaactg ccgccagtaa tttacgcgta taaggatgct gcgggttttc
     3781 gaagaccgcg cgccgtggac caatttcaac aatttgcccg agatacatca ccgccacacg
     3841 atgactaatc cgctctacca cagccatatc gtgggagata aacagatacg caatgccgaa
     3901 atcacgctgg agatcgagca acaagttgat aatctgcccg cgaatagaaa catccagcgc
     3961 cgaaacggct tcgtcggcaa tgatcacttt tggattcaat gccaacgcgc gagcaatgca
     4021 gatgcgctgg cgctgaccgc cggaaaactc atgcgggtaa cgccaggcat gttcaggtaa
     4081 caggcccacg cgctccagca accacgcaac gcgtgcaacc gcttctttac ctggcaataa
     4141 accgtgtaca cgcaacggtt cgataatcga atcaccgatg gtctgacgtg ggtccagcga
     4201 agcgtaaggg tcctgaaaaa taaactgaat atcgcggcgc aatacctgaa gtttgccggg
     4261 tgacaaggta tcgattcgct gaccgttaaa gataatttcg ccgccctgcg attcgaccag
     4321 gcgcagcaac gcccgcccgg tagtggattt accgctgcca gactcgccca ccagcgatag
     4381 cgtttcgcca ggccagagat caaaactgac tttctcaacg gcatgcactt cccgcgttac
     4441 gcgattcaac aaaccgctgc gcaaagggaa acgggtgacc agattacgca cccgtaaaac
     4501 aggttcgcca tccaccaccg ttttctgctc gatggggggt tcctgtttcg ctggatgttc
     4561 aagcgatatc aacgggaaac gtcggggata atctaaccct ttcatcgcac caagttgcgg
     4621 aacagcagct aacagcgcac gggtgtaagg atgttgcggt gcatgaaaaa tctgttcgac
     4681 ggtacccgtt tccaccgcct cgccctgata catcaccagt acccgatcgg caatctctgc
     4741 caccacgccc atatcgtgag tgataaagat aacgcccatc gacatctctt tttgcaatac
     4801 tttgattaat tgcaggatct gcgcctgaat agtgacatcc agcgcggtgg ttggctcatc
     4861 ggcaatcagc accgccgggc ggcatgacag cgccatcgca atcatcactc gctggcgcat
     4921 cccgccagag agttgatgcg gataacgtga aagaatggtt tgtgcctccg gaatgcgtac
     4981 ctgatccagc atccgcttcg cctcgaccat cgcttcttca cgactggcgt tttgatgcag
     5041 acgaattgat tcggcaatct gttcacccac agtaaatacc gggttcagcg atgtcatcgg
     5101 ctcctgaaat atcatcgcca tatccgcacc gcgcacatga cgcatttgtg cagcgctctg
     5161 ctcgctaagt tcaatcactt cacgactgcg ccgccgcaac agcattttat cgcactgtac
     5221 taaaccgccc gcctgttcca acagacgcat caatgccagc gcagtcactg acttaccgga
     5281 gccggattcg ccaacaattg ccagcgtctc accgcgttgc aggctaaaag agagattgcg
     5341 gaccgcagct attttctgct ggtcctgcat aaaggcaata ttaagatttt caaccgccag
     5401 cacattaccg gcatcaagtt catcactgtg tggcaacggc gtcccctttt tcacggtaga
     5461 taccggtggt tggcgtatcg cctgcgtagc cccaggcgcg atacattcct tcggtgttaa
     5521 acggtagcgc gacattccct tcatggtcga tagcgattaa gccaccgcta ccgccaagcg
     5581 cagggagttt ttccattact acccgctcgc aggcttccgc gagacttaat ccgccgtaat
     5641 ccattaacgc ggcgatgtca tatgccgcca gcgcgcggat gaagacttcg cccgtgccgg
     5701 tacaagaaac cgccacactg gcgttattgg cgtagcatcc ggcacccact aaggggctat
     5761 cgccaactcg tccgggtaat ttattggtca ttccgcccgt ggacgtggct gccgccagat
     5821 tgccgtctaa atccaacgcc acggccccca cggtgcccat tttctgtttt tcatccagtg
     5881 gcgcaccgct atggtcgagg actgttgccc cttcctcgcg cgctgccatt agttgttcat
     5941 aacgcaaagg cgtggagaaa atctccggtg agacgcgctc catgccatga gcaaacgcaa
     6001 aattttctgc cccttcgcca atcatcatca catgcgagct ttgctccatc accagccggg
     6061 cggcaagaac cggattacgc agatgactaa cgcccgccac cgcaccggct ttcagggtgt
     6121 taccatccat cacacaggcg tccagttcat gcgtttcatc acgcgtaaag acggcgccaa
     6181 ttccggcgtt aaacagtgga cactcttcca gcagacgcac cgcttccgtc accacatcca
     6241 gcgcactttc gcccgcttcc agcattttct gcccggtttc aacaatggca gacaacgcct
     6301 cgatgtagcg taattcctgt tgcagactca tctgcgcgcg gctaattgca cctgcgccac
     6361 catgaattgc aatgactgct ttgcccatag tattcgtcca ttaagggtgg ctcgccactt
     6421 ttgctataaa tatcagaatt tttatgaatt actcattcaa tttttgaata tagaaagatg
     6481 gatatggcat gtaaaggcag gacccagcgc tacgcccgag gtggcataca gtctgtggcg
     6541 tttttctgac ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt
     6601 accaccggat tgatgtcgct cgacaccgcg cttaatgaga tgctttctcg cgtcacccca
     6661 ctgaccgccc aggaaacgct gccactggta cagtgttttg gtcgtattct ggcgagcgat
     6721 gtcgtttcgc cgcttgatgt tccggggttt gataactccg caatggacgg ctacgcggtg
     6781 cgtttagccg atattgcctc cgggcaaccg ctgcccgttg ccggtaaatc ctttgccggt
     6841 cagccgtacc atggtgaatg gcctgcgggt acctgcattc gtattatgac cggtgcgccg
     6901 gtgccggaag gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc
     6961 gtgcgtttta ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc
     7021 tctgcaggtg cggttgtttt cccggcgggg actcgcctga ctaccgcaga gctgccagta
     7081 attgcttcac tggggattgc cgaagttccg gtgattcgta aagtgcgagt ggcgcttttt
     7141 tctaccggtg atgaactcca gttgcccggt cagccgctgg gcgacggcca aatctacgat
     7201 accaaccgtc tcgccgtaca cctgatgtta gaacagctgg gttgcgaggt aattaactta
     7261 gggattatcc gcgatgatcc ccatgccctg cgcgccgcat ttattgaagc cgacagccag
     7321 gcggatgtgg tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacg
     7381 attcttgaag agctggggga gatcgccttc tggaagctgg cgattaaacc aggtaaaccg
     7441 ttcgcgttcg gtaaactcag caatagctgg ttctgcggcc tgccgggcaa cccagtttca
     7501 gcgacgctga ccttctatca actggtacag cctttgctgg caaaactaag cggtaacacc
     7561 gccagcggcc tgcccgcgcg ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca
     7621 ggtcgccttg atttccagcg cggcgtgctg caacgcaacg ccgatggcga actggaagtg
     7681 acgaccactg gacatcaggg ttcacatata tttagctcct ttagcctcgg caactgcttt
     7741 atcgtactgg aacgcgatcg cggcaatgtg gatgtgggcg aatgggtgga agtagaaccg
     7801 tttaacgcgt tgttcggagg cctgtaatgg cggaactcag cgatcaggag atgctgcgct
     7861 acaaccggca aatcattctg cgcggttttg attttgacgg tcaggaggcg ctaaaagatt
     7921 ctcgtgtgct ggtagtcggc ctcggtggac tcggctgcgc agcttcgcag tatctggcaa
     7981 gcgccggtgt cggcaacctg acgctgctcg acttcgactc ggtttcgctc tcgaatctgc
     8041 aacgccagac gctgcacagt gatgccacgg tcgggcaacc gaaggtggaa tccgcccgtg
     8101 acgccctggc gcggatcaac ccacatatcg cgattacgcc agtcaatgca ctgctggatg
     8161 acgcagaact tgcagcattg attgctgaac acgatctggt gctcgactgt acggataacg
     8221 ttgcggtacg taatcaactg aacgcaggat gttttgccgc gaaggtgccg ctggtttccg
     8281 gcgcggcaat tcgtatggaa ggtcaaatca cggtcttcac ttatcaggac ggcgaaccgt
     8341 gctatcgctg ccttagccgt ttgtttggcg agaacgcgtt aacctgcgta gaagcaggcg
     8401 tgatggcacc attgatcggc gtaattggtt cgttgcaggc aatggaagcg atcaagctgc
     8461 tggcaggtta tggcaaaccg gccagcggaa aaatcgtgat gtacgatgcg atgacctgtc
     8521 agtttcgcga aatgaaactg atgcgcaatc cggggtgtga agtgtgcggg cagtaattgc
     8581 cacctccagg cctgataaga cgcggcactc cgccacgtca tcagcaattt atgaggatgc
     8641 agaacgtccg gttaaatcga cgttctgcca aacgctccct gccagtcctg ctcaaacttc
     8701 gccacagcgg catcaaccgc cggatagcta atcatctgtt gtgccacatc cagtggcaga
     8761 gtaattgatt cacatcctgc cagtaagcag tccagcgcct gacgcggggt tttgaaactc
     8821 gctgccagca ctttcgcctg cggcgcatgc attttcaata actggtgtaa gtcggtcaca
     8881 gtctgaatgc cgctaccgcc ctgagcatca atacgattaa cgtaaggcgc aacatattcc
     8941 gcacctgcca gcgccgacag caacccttgt gccgcgccat ataccgcggt tcccagcgtc
     9001 ggaatccctt ccgcttttaa catcttaatc gctgccagcc cctcggcggt caccggaact
     9061 ttcaccacga tatccgcaat aatagaacgc aatttacgtg cgtcattcac catcccttca
     9121 gcagtgatag ccattacctg ggcaaacaga cgcccctgac cgcccatcgc ttcatgaagt
     9181 tccggaagca caacctccag cggtttttta cccgcggcga taatgcttgg gttagtggtc
     9241 acgcccgcca gcggaaaaat acgtgacagc gccttcaccg caacaacgtc tgaagtatcc
     9301 agatacagtt ccataaccat cctcaaaatg tactttttgt ctgaatgtag actatcacgg
     9361 gaaaactctc tgaacagttg acgcacatca atctaacttt cattcgaaag tattttaatg
     9421 tttgaacgaa acagagaggc aactatgatt ttcaatattc agcgctactc gacccatgat
     9481 ggccccggta tccgcacggt cgtatttctt aaaggctgtt cgctgggctg ccgctggtgt
     9541 cagaacccgg aaagccgcgc ccgcacgcag gatctgctgt atgacgcacg actgtgtctg
     9601 gaaggctgcg agctgtgcgc taaggcagcg ccggaagtga ttgagcgcgc gctgaatggt
     9661 ttgcttattc atcgggaaaa gttaaccccg gagcatctga cggcgttaac cgactgctgc
     9721 ccgacacagg cattaaccgt gtgtggtgaa gtgaaaagcg ttgaggagat catggcgacc
     9781 gttttgcgcg ataaaccgtt ttacgatcgc agcggcggag gcttaacgct ttcgggtggt
     9841 gaacccttta tgcagccgga aatggcgacg gcgctactgc aagccagcca cgaggccggc
     9901 attcatactg cggtagaaac ctgtctgcat gtgccgtgga aatatatcgc cccttctctg
     9961 ccctatatcg atctgtttct tgccgattta aaacacgttg ccgacgcgcc gtttaaacag
    10021 tggaccgacg gtaacgccgc cagagtgctg gataacctga aaaaactcgc cgcagcgggc
    10081 aaaaaaatca ttatccgcgt gccgctgatt cagggcttta atgccgacga aacctctgta
    10141 aaagccatta ccgattttgc cgccgacgag ctgcacgttg gcgaaattca tttcctgccc
    10201 taccacacgc tgggcatcaa caaatatcac ttacttaatc tgccctatga cgccccggaa
    10261 aaaccgcttg atgcgccaga actgctcgac tttgcccagc agtatgcctg ccagaaaggg
    10321 ttaaccgcga ccttacgagg ataacaacca tgaccacact gaaactggac acgctcagcg
    10381 accgcattaa agcgcacaaa aatgcgctgg tgcatattgt gaaaccgccg gtttgtaccg
    10441 agcgcgcgca gcactatacc gagatgtatc aacaacatct cgataagccg atccctgtac
    10501 gtcgcgcgct ggcgctggca catcacctgg cgaatcgcac tatctggatc aagcacgatg
    10561 agttgatcat cggcaaccag gcaagcgaag ttcgcgccgc gccgatcttc ccggaatata
    10621 ctgtctcgtg gatcgaaaaa gagattgatg atctggcaga tcgtcccggt gctggctttg
    10681 cggtgagcga agagaacaaa cgcgttctgc atgaagtgtg cccgtggtgg cgcggtcaga
    10741 ccgtacagga tcgctgctac ggcatgttta ccgatgagca aaaaggtctg ctggcgaccg
    10801 gcatcattaa agcggaaggc aatatgacct ccggcgatgc gcacctggcg gtcaatttcc
    10861 cgctgctgct ggaaaaaggg cttgatggtc tgcgcgagaa agtagcggaa cgtcgctcgc
    10921 gcatcaacct gacggtgctg gaagatttac acggcgagca gttcctgaaa gcaattgata
    10981 tcgtgctggt ggcagtcagt gaacacattg aacgtttcgc tgccctggcg cgtgaaatgg
    11041 ccgcgaccga aacccgcgaa agccgtcgcg atgaactgct ggcgatggca gaaaactgcg
    11101 atcttatcgc ccaccagccg ccgcagacgt tctggcaggc gctgcaactg tgttacttca
    11161 tccagttgat attgcagatc gaatctaacg gtcactcggt atcgtttggt cgtatggacc
    11221 agtatctcta cccttactat cgccgcgacg ttgaactcaa ccagacactg gatcgcgaac
    11281 acgccatcga attgctgcat agctgctggc tgaaactgct ggaagtgaac aaaatccgct
    11341 ccggctcaca ctcaaaagcc tctgcgggaa gtccgctgta tcagaacgtc accattggcg
    11401 ggcaaaatct ggttgatggt caaccaatgg acgcggtgaa tcccctctct tacgcgatcc
    11461 ttgaatcctg cggtcgcctg cgttccactc agcctaacct cagcgtgcgt ttccatgcag
    11521 gaatgagtaa cgatttcctc gacgcctgcg tacaggtgat ccgttgcggc ttcgggatgc
    11581 cggcgttcaa caacgacgaa atcgtcatcc cggaatttat taaactcggt attgaaccgc
    11641 aggacgctta cgactatgca gcgattggtt gtatcgaaac cgccgtcggt ggcaaatggg
    11701 gctatcgctg taccggcatg agctttatca acttcgcccg cgtgatgctg gcggcgctgg
    11761 aaggcgggcg tgatgccacc agcggcaaag tgttcctgcc acaagaaaaa gcgttgtcgg
    11821 caggtaactt caacaacttc gatgaagtga tggacgcgtg ggatacgcaa atccgttact
    11881 acacgcgtaa atcaatcgaa atcgaatatg tcgtcgacac catgctggaa gagaacgtgc
    11941 acgatattct ctgctcggcg ctggtggatg actgtattga gcgagcgaaa agtatcaagc
    12001 aaggcggcgc gaaatatgac tgggtttctg gcctgcaggt tggcattgcc aacctcggca
    12061 acagcctggc ggcagtgaag aaactggtgt ttgaacaggg cgcgattggt cagcaacagc
    12121 tggctgccgc actggcggat gacttcgatg gcctgactca tgagcaactg cgtcagcggc
    12181 tgattaacgg cgcgccgaag tacggcaacg acgatgatac cgtcgatacg ctgctggctc
    12241 gcgcttatca gacctatatc gacgaactga aacagtacca taacccgcgc tacggtcgtg
    12301 gtccggttgg cggcaactat tacgcgggta cgtcgtctat ctccgctaac gtaccgtttg
    12361 gcgcgcagac tatggcaacg ccggacggac gtaaagcgca taccccgctg gcagaaggcg
    12421 caagcccggc ttccggtact gatcatcttg gccctactgc ggtcattggc tcggtgggta
    12481 aactgcctac ggcagcgatt ctcggcggcg tgttgctcaa ccagaaactg aatccagcca
    12541 cactggagaa cgaatctgac aagcagaaac tgatgatcct gctgcgcacc ttcttcgagg
    12601 tgcataaagg ctggcatatt cagtacaaca tcgtttcccg cgaaacattg ctggaggcga
    12661 aaaaacatcc ggatcaatac cgcgatctgg tagtgcgtgt cgcgggctat tccgccttct
    12721 tcaccgcgct ctctccagac gcacaggacg atatcatcgc ccgtactgaa catatgctgt
    12781 aatctcctcg gcccggcggc gtcgtcgccg ggctaaatca ttcacatcat caatttcatc
    12841 cttactttca ttcgaaatat aatttgtgct ctgcgtcaca ttgttattac actgttacgg
    12901 tttcattttc gagccaggag cacacctatg agcgtaaaag ttatcgtcac agacatggac
    12961 ggtacttttc ttaacgacgc caaaacgtac aaccaaccac gttttatggc gcaatatcag
    13021 gaactgaaaa agcgcggcat taagttcgtt gtcgccagcg gtaatcagta ttaccagctt
    13081 atttcgttct ttcctgagct aaaggatgag atctcttttg tcgcggaaaa cggcgcactg
    13141 gtttacgaac atggcaagca actgttccac ggcgaactga cccgacatga atcgcggatt
    13201 gtgattggcg agttgctaaa agataagcaa ctcaattttg tcgcctgcgg tctgcaaagt
    13261 gcatatgtca gcgaaaacgc ccccgaagca ttcgtcgcac tgatggcaaa acactaccat
    13321 cgcctgaaac ctgtaaaaga ttatcaggag attgacgacg tactgttcaa gttttcgctc
    13381 aacctgccgg atgaacaaat cccgttagtg atcgacaaac tgcacatagc gctggatggc
    13441 attatgaaac ccgtcaccag tggttttggc tttatcgacc tgattattcc cggtctacat
    13501 aaagcaaacg gtatttcgcg gttactgaaa cgctgggatc tgtcaccgca aaatgtggta
    13561 gcgattggcg acagcggtaa cgatgcggag atgctgaaaa tggcgcgtta ttcctttgcg
    13621 atgggcaatg ctgcggaaaa cattaaacaa atcgcccgtt acgctaccga tgataataat
    13681 catgaaggcg cgctgaatgt gattcaggct gtgctggata acacatcccc ttttaacagc
    13741 tgacctctct accggagcca tgcgctccgg ttttcccttc tttttacatc caacacatca
    13801 aatcattaaa cttgcattaa cttatttata acttatattg tcacatgcaa atcagcacag
    13861 tttcgaatga aagttaatgc gaggtggtta tggctcctac ttttaccagc gacacattgc
    13921 ctgccgatca caaagcagct atccgtcaga tgaagcacgc gctgcgggcg cagcttggcg
    13981 acgtccagca gatctttaat cagctaagcg atgacattgc cacgcgagtg gctgaaatca
    14041 acgcactcaa agcacagggc gatgccgtct ggccggtgct gtcttatgcc gatatcaaag
    14101 caggtcatgt tactgcagag cagcgcgaac agattaaacg tcgcggttgt gcggtgataa
    14161 aaggccattt cccccgcgaa caagcgctag gctgggatca gtcgatgctg gactatctgg
    14221 accgcaaccg ctttgacgag gtctacaaag gccccggcga taatttcttc gggacgctca
    14281 gcgcttcacg tcccgagatt taccccatct actggtcgca ggcgcaaatg caggctcgcc
    14341 agagtgaaga aatggcgaat gcgcagtcgt ttctcaatcg tctgtggaca tttgaaagtg
    14401 atggaaagca atggtttaac ccggatgtga gcgtcatcta ccctgaccgt atccgccgcc
    14461 gtccgcccgg aacgacctcc aaaggtcttg gagcgcatac cgactccggg gcactggaac
    14521 gctggctgct tccagcgtat cagcacgttt tcgctaacgt ctttaatggc aatctggcga
    14581 aatacgatcc ctggcatgcg gcacatcgta cggaagttga agagtacacg gtagataaca
    14641 ctacaaaatg ttcggtgttc cgcacgtttc aaggctggac ggcgctctct gatatgttat
    14701 cgggtcaggg attgttacat gtggtgccta tcccggaagc gatggcgtat gttttattgc
    14761 gcccgctgct ggatgacgta ccggacgatg aactatgcgg ggttgcacct ggcaaagttc
    14821 tgcccatttc agagaaatgg catccactac tgcttaaagc gctgacttcc attcccgcga
    14881 taacagcagg ggattccgtc tggtggcact gcgatgtgat tcactccgtc gcgccagtag
    14941 acaatcaaca gggttggggc aacgtgatgt atatccctgc tgcgccaatg tgcgagaaaa
    15001 atcgggctta tgcacacaaa gtcaaaatgg cgctggaaaa aggcgcatcg ccgggcgact
    15061 tcccgcgcga ggactatgaa acaaactggg aaggacgctt tacgcttgcc gacctcaaca
    15121 ttcacggtaa gcgcgcactg ggcatggatg tttgagaatt aatggaagtg aattgatgtg
    15181 ggattaagaa aatcgattcg acgttactca gattgctgac aagtgcgctt tcttaatgcc
    15241 tgatgcggcg ttaacgcctt atctggccta caaagtcctg cagattcaat atattacagg
    15301 tactgcgtag gcctgataag cgtagcgcat caggctattt tgcatttgtc atcagccgcc
    15361 gtcaccccga cagtatcggg gtgacggata ataacttact cgatcccttt actacgcagg
    15421 taatcttcgt aattaccgct aaagtcgatc acgcgttccg gggtgatttc cagaatgcgg
    15481 gtcgccaggg agcttacgaa ctcacggtcg tgtgaaacaa agatcagcgt gccctgatac
    15541 agttccagcg ccatgttcag tgactcgatg gattccatat ccaggtggtt ggtcggttcg
    15601 tccatgatca gaatgttcgg cttctgcatc attaacttac caaaaagcat ccgccctttt
    15661 tctccaccgg aaagcacttt agctggcttt ttgatgtcgt cctggctgaa cagcaaacga
    15721 ccgagaatgc tgcgtaccgc ctgctcgtca tcgccttcct gcttccactg gctcatccat
    15781 tcgaacacgg tcagatcatt ttcaaactca tattcgtggt cctgagcata gtaaccaatg
    15841 cgcgcgtttt cagaccattt tacggtgccg ctgtccggtt gcagatcacc caccagcgtt
    15901 ttcagcagcg ttgatttacc gacgccgttg gtacccagta ccgcaagttt ttcaccgacc
    15961 tccagcagca ggttgagatt tttaaacagt ggaccgttat caaacccttt ggtcagacct
    16021 tccacttcca gcgcgttacg gaacagtttc ttatcctgtt caaaacggat gaacgggttc
    16081 tgacggctgg aggctttcac ctcttccagt ttgattttat caatctggcg cgcgcgcgaa
    16141 gttgcctggc gagatttcga ggcgttggcg ctaaagcggc taacgaaaga ttgcagctca
    16201 gcaatctgcg ctttcttctt ggcgttatca gccagcagac gttcacgcgc ctgggtcgcc
    16261 gccgtcatgt actcatcgta gttgcccgga taaacgcgca gctcgccgta atccagatcc
    16321 gccatgtggg tacaaaccat gttaaggaag tgacggtcgt gcgagatgat gatcatggtg
    16381 ctgtcacgct cgttcagcac ctgttccagc cagcgaatgg tgtcgatgtc gaggttgttg
    16441 gtcggttcgt cgagcaggag aatatccgga tcagcaaaca gcgcctgcgc cagcagcaca
    16501 cgcagcttcc agccaggagc aacttcactc atcgggccgt agtgctgttc cactggaatt
    16561 cccacgccaa gcaacaattc accggcgcga gcttccgcag agtaaccgtc catttcgccg
    16621 tatttaactt ccagatcggc cactttatag ccgtcttctt cactcatttc cggcaaagca
    16681 tagatgcggt cgcgctcctg cttcacttcc cacaactctt tatgccccat gatcaccgta
    16741 tccagcacag tgaactcttc aaaggcaaac tgatcctgac gcagcttacc aatgcgctcg
    16801 ttgggatcga gggaaacgtt acccaacgtc ggctctaagt cgccgccgag gatcttcata
    16861 aaggtggatt taccactacc gttcgcgcca atcaggccgt aacggttgcc gccgccaaat
    16921 ttgacggaaa tgttttcaaa caacggctta ctgccgaact gcatggtgac gttactggaa
    16981 actaacacag gcgtatcctg aaaagagata tgacaaaccg cgcattatgc cataagtctg
    17041 aaagaagatc ctgctcctcg cttaaggtct acactcaagc caaaggcaaa aaatgtgatt
    17101 tcgtacacat ctgatttcac tgtgagctgg aatgaactta taatgcgctt ccaatactct
    17161 aatattctca acccaatggc ctgccaggca caaaatctcg cttaacatga atatgaaatt
    17221 gaaaacatta ttcgcagcgg ccttcgctgt tgtcggcttt tgcagtaccg cctctgcggt
    17281 aacttatcct ctgccaaccg acgggagtcg cctggttggt cagaatcagg tgatcaccat
    17341 tcctgaaggt aacactcagc cgctggagta ttttgccgcg gagtaccaga tggggctttc
    17401 caatatgatg gaagcgaacc cgggtgtgga taccttcctg ccgaaaggcg gtactgtact
    17461 gaacattccg cagcagctga tcctgccgga taccgttcat gaaggcatcg tcattaacag
    17521 tgcagagatg cgtctgtatt actatccgaa agggaccaac accgttatcg tgctgccgat
    17581 cggcattggt cagttaggca aagatacgcc tatcaactgg accaccaaag ttgagcgtaa
    17641 gaaagcaggc ccgacctgga cgccgaccgc caaaatgcac gcagagtacc gcgctgcggg
    17701 cgaaccgctt ccggctgtcg ttccggcagg tccggataac ccgatggggc tgtatgcact
    17761 ctacatcggt cgcctgtatg ctatccatgg caccaacgcc aacttcggta tcggcctgcg
    17821 tgtaagtcat ggttgtgtgc gtctgcgtaa cgaagacatc aaattcctgt tcgagaaagt
    17881 accggtcggt acccgcgtac agtttattga tgagccggta aaagcgacca ccgagccaga
    17941 cggcagccgt tatattgaag tccataatcc gctgtctacc accgaagccc agtttgaagg
    18001 tcaggaaatt gtgccaatta ccctgacgaa gagcgtgcag acagtgaccg gtcagccaga
    18061 tgttgaccag gttgttcttg atgaagcgat taaaaaccgc tccgggatgc cggttcgtct
    18121 gaattaatcg tcaaaacgta aaacaaaggc ggactcataa tccgcctttt ttatttgcca
    18181 gaccttacca ggccgggatc ataaccaaca aaacatatcc caccagcgcc gcccacaaca
    18241 gcatcggtat tgaatagaga tggaaacgcc accagatgcg gcgatcgttc gccatacgta
    18301 gcgcaatcaa atttgccagc gatccgggta ataacccaaa gccacctacg tttaccgccc
    18361 atgccagtaa taaagacggc ggcacatagt tcagcaacaa tatggtactc ggcacgttac
    18421 tgatcacctg cgataaaccg attgccgtta accataaccc gggttcagat aaatgactca
    18481 cgttacccaa cacgccttgc aacgctggaa gctggatcag taaatggacg tcgataaaca
    18541 tcgccataaa caccagcagc agcgtccagt ccacactcaa caccacgcga cgtgccagca
    18601 gcgcaaagcc caccgccaca atcaccagtc cccacagctc ttgttttaac tccagcgccg
    18661 tcagaaagac gatatacagt cccagacaac tccacaccag ccgcggtttc cactccggcg
    18721 tttgcacccc cgtatggtat tgcagcgcct ttccagggaa acaacaccag cacaacagca
    18781 ggagtgtcag catcattgca ccagccagcg gtgccatttg ggcaataaat ccggcaaacg
    18841 aaagaccaga acgtccccag ataagaatat tttgcgggtt gccaattggc gtcagtagcg
    18901 aaccagcatt gactgccagc gcctcaaaaa taatcagccg attaaccggg atctcacaca
    18961 gtctttttag cgtgatagtc agcggaacaa caataaacag cgcgacatcg ttggtcagaa
    19021 aggtagaaag cagcgccgcc gccagcacca taaacatcgc cagccgacgc tccgtagcaa
    19081 agcggcgcac cattttgcgc cccagcacat caaaataacc gcttaactcc acacctttgg
    19141 tcagcagcat caggccgctt aatgtgatga tggtgtgcca gtcgatagca gcaggccagg
    19201 atttcggtgc aaagggcacg aaaaagctta atccgatacc aacaagaatt aataactgaa
    19261 aaaaacgatc cccttgcagc gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg
    19321 ggtaaacaaa cgaaaggcat ccagcgtctc ttcactaaca tggtgctcca tgccttccgc
    19381 gtcgcgacgg gcgatttccg gactgacgcc caacaccaat aagaaatttt cgactatctg
    19441 atgtcgctcg cggctttcct gcgccagctt ctctccttct gccgttaaaa acacgcctcg
    19501 ccaggggatc atttcaatca gccccatggt tgccagtcgc ttaagcattt tagccaccgt
    19561 cggttgcgaa actcccaaac gagcagccat gtctacctga cgagcttccc ccacttccct
    19621 gatcaagtca gaaatcagct caacgtaatc atcaataagc tcgcgccgat gcgcctctct
    19681 gacctggcgg aacccttcaa cgtgctcttc cacgttcact aactgcgtca ctttttttgc
    19741 tgttggcgta cctgcgcgac gactcattgt gcttcctcat tatggtgacg catacatagc
    19801 gttctcattt cgtgcgcaca ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt
    19861 tagcaatagc tatataatat agcctgtgct atatctgtat gtaatgcaat catccctcaa
    19921 ggatcgacgg gattagcaag tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg
    19981 tgtttagtca ttctcccttt aaagtacggt taatgctgct ctctatgttg tgcgatatgg
    20041 tcaacaacaa accgcagcaa gataaacctt ccgataaata gcggcgtcgc ggtacgccgc
    20101 ttcactcctg ctttcatgca ggcataacgc gttttggtct gaaaaacccc actttttgtc
    20161 ggatttgcaa tccccttcgc aaaagatttg ttcgtcagta gttgacctga acggcggctc
    20221 gctctatctt cttgcagccc tgcgtatatt gcggctcgcg gatgcggacc cctttcccct
    20281 cttcacgcac tcttgcaggt attgaccctt gacgccaggg taagcacatg gcgtttgtta
    20341 cgatagtggc atatgaattt aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt
    20401 agcccgtgga ctgcgttcta ctttttacag tcgctattaa ttaacctcgg cttaggttat
    20461 cccttcagtt tgctctacac cgctgcgttt acggctattt tgcttttgct atggcgaaca
    20521 ttgcctcgcg tacaaaaagt tctggtcggt gtcagttcgc tggtggcggc ttgctatttc
    20581 ccttttgctc aggcctacgg cgcgcctaat ttcaatacat tgctggcatt gcactccacc
    20641 aatatggaag agtcgaccga aatcctgacg atttttccgt ggtacagcta cctggtcggc
    20701 ttatttattt ttgcgctcgg cgtaatagca atcaggcgaa aaaaagagaa tgaaaaagcg
    20761 cgctggaata ccttcgacag cctgtgtctg gtattcagtg tggcgacatt ttttgttgct
    20821 cccgtgcaaa acctggcctg gggtggcgta tttaaactga aagatactgg ctatccggta
    20881 tttcgttttg ctaaggatgt catcgtcaat aataacgagg tgattgaaga gcaagaacgg
    20941 atggcaaaac tttccggaat gaaagatacc tggacggtca ctgccgttaa gccgaagtat
    21001 cagacctatg tggtggtgat cggtgaaagc gcgcgtcgcg atgccctcgg tgcctttggc
    21061 ggtcactggg acaatacccc gtttgccagc agcgttaacg gtttgatatt tgctgactac
    21121 attgccgcca gtggctccac gcagaaatcg cttggcttaa cgctcaatcg cgttgtcgat
    21181 ggcaagccac agtttcagga taactttgtc accctggcaa atcgcgcggg cttccagacc
    21241 tggtggtttt ccaaccaggg ccaaatcggc gaatacgata ccgccatcgc cagcatcgcc
    21301 aaacgagcag atgaagtgta cttccttaaa gatggtaatt ttgaagcaga taaaaacacc
    21361 aaagacgaag cgttactgga tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg
    21421 cagctgattg ttctgcatct gatgggctcg catccgcagg cctgcgaccg gacacaaggc
    21481 aaatatgaaa cttttgtgca atcgaaagaa acgtcgtgct atctctatac tatgacgcaa
    21541 acggacgatt tactgcgcaa gctgtacgat cagttacgca acagcggcag cagcttctcg
    21601 ctggtttact tttctgacca cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac
    21661 cttgcccatg atgataaata tcagcaaaat ttccaggtgc cttttatggt catttccagc
    21721 gacgataaag cgcatcgtgt gattaaagcc cgccgctcag ccaatgactt cttaggcttt
    21781 ttctcccagt ggacgggaat taaagcgaag gaaattaaca tcaaataccc gtttatatct
    21841 gagaagaaag ccgggccgat atacatcacc aacttccagt tacagaaggt ggattacaac
    21901 catctcggaa ccgatatttt cgacccgaaa ccttaaaaac aaaaatccgc cccgagaggc
    21961 ggattttttt atatcaccaa agtgattaga agcggtaacc aacaccggca atccaggtgc
    22021 ctacgtcaac gctacgaata cggctctgct cgtaagagaa gtccagagca acgttttcca
    22081 tcgggttgaa ctgcagacca gcaccgtagg agaaaccgta gtcgctggtg tcgtgtttgt
    22141 aggtcgggta ttcagtggtc tggaatttac cataacccac acccactaca ccgtagatgc
    22201 ttgcccagtc gttaatgcgg taagccggac cagcagtgat gccgtagtac tggtttttgt
    22261 tgtagtcacc agagcttgca gtacggcttt tctcggtgta agtgaaagaa ccgatcacac
    22321 ccagcgggct gttgtcttct tcatagcggt atttcaggtt gaaaccgccc attttgttca
    22381 tttggccctg agcgtcgctc tgtgcgtaac cgccagttac ggtagaagtc gcagctacgg
    22441 aagtacctgc ggtgaaagcc agaactgcgg ccagtgctga aagacatgca atttttttca
    22501 taaccacctc aaatgtgatt caaataagtc ctaagtttta aatatatcaa aaattaatgg
    22561 gaaactcttc gcgatttgtg atgtctaacg ggccatttca tgtaacagaa cgtttccata
    22621 caccgctatc catctaaatt taaatcactt tttcagagaa ctgcgtaagt attacgcatg
    22681 ttttccctgt cattcatcca gattattcct aatcaccaga ctaatgattc catcaatcct
    22741 ggcgcatttt agtcaaaacg ggggaaaatt ttttcaacaa atgctcaacc agcattgggt
    22801 atatccagta cactccacgc tttacttaag tctagatatt tgtgggagaa aggatgcctg
    22861 gttcattacg taaaatgccg gtctggttac caatagtcat attgctcgtt gccatggcgt
    22921 ctattcaggg tggagcttcg ttagctaagt cactttttcc tctggtgggc gcaccgggtg
    22981 tcactgcgct gcgtctggca ttaggaacgc tgatcctcat cgcgttcttt aagccatggc
    23041 gactgcgctt tgccaaagag caacgattac cgctgttgtt ttacggcgtt tcgctggggg
    23101 ggatgaatta tcttttttat ctttctattc agacggtacc gctgggtatt gcggtggcgc
    23161 tggagttcac cggaccactg gcggtggcgc tgttctcttc tcgtcgcccg gtagatttcg
    23221 tctgggttgt gctggcggtt cttggtctgt ggttcctgct acctctgggg caagacgttt
    23281 cccatgtcga tttaaccggc tgtgcgctgg cactgggggc cggggcttgt tgggctattt
    23341 acattttaag tgggcaacgc gcaggagcgg aacatggccc tgcaacggtg gcaattggtt
    23401 cgttgattgc agcgttaatt ttcgtgccaa ttggagcgct tcaggctggt gaagcactct
    23461 ggcactggtc ggttattcca ttgggtctgg cggtcgctat tctctcgacc gctctgcctt
    23521 attcgctgga aatgattgcc ctcacccgtt tgccaacacg gacatttggt acgctgatga
    23581 gcatggaacc ggcgctggct gccgtttccg ggatgatttt cctcggagaa acactgacac
    23641 ccatacagct gctggcgctc ggcgctatca tcgccgcttc aatgggttct acgctgacag
    23701 tacgcaaaga gagcaaaata aaagaattag acattaatta aatttacat
//
LOCUS       DAZKFU020000050        23458 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZKFU020000050 DAZKFU020000000
VERSION     DAZKFU020000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.050"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..3871)
                     /gene="entF"
                     /locus_tag="ACHOQQ_RS07890"
                     /old_locus_tag="pgaptmp_001578"
     CDS             complement(<1..3871)
                     /gene="entF"
                     /locus_tag="ACHOQQ_RS07890"
                     /old_locus_tag="pgaptmp_001578"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGU4469846.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLAAQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHSERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFSSLLGCDVQDVDADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRAT"
     gene            complement(3868..4086)
                     /gene="ybdZ"
                     /locus_tag="ACHOQQ_RS07895"
                     /old_locus_tag="pgaptmp_001579"
     CDS             complement(3868..4086)
                     /gene="ybdZ"
                     /locus_tag="ACHOQQ_RS07895"
                     /old_locus_tag="pgaptmp_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HGU4469847.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(4089..5291)
                     /gene="fes"
                     /locus_tag="ACHOQQ_RS07900"
                     /old_locus_tag="pgaptmp_001580"
     CDS             complement(4089..5291)
                     /gene="fes"
                     /locus_tag="ACHOQQ_RS07900"
                     /old_locus_tag="pgaptmp_001580"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HGU4469848.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLEAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5534..7797
                     /gene="fepA"
                     /locus_tag="ACHOQQ_RS07905"
                     /old_locus_tag="pgaptmp_001581"
                     /pseudo
     CDS             5534..7797
                     /gene="fepA"
                     /locus_tag="ACHOQQ_RS07905"
                     /old_locus_tag="pgaptmp_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
     gene            7972..8592
                     /gene="entD"
                     /locus_tag="ACHOQQ_RS07910"
                     /old_locus_tag="pgaptmp_001582"
     CDS             7972..8592
                     /gene="entD"
                     /locus_tag="ACHOQQ_RS07910"
                     /old_locus_tag="pgaptmp_001582"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGU4469849.1"
                     /translation="MKTTHTSLPFAEHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8714..8866)
                     /gene="hokE"
                     /locus_tag="ACHOQQ_RS07915"
                     /old_locus_tag="pgaptmp_001583"
     CDS             complement(8714..8866)
                     /gene="hokE"
                     /locus_tag="ACHOQQ_RS07915"
                     /old_locus_tag="pgaptmp_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU4469850.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            8835..9103
                     /locus_tag="ACHOQQ_RS07920"
                     /old_locus_tag="pgaptmp_001584"
                     /pseudo
     CDS             8835..9103
                     /locus_tag="ACHOQQ_RS07920"
                     /old_locus_tag="pgaptmp_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9318..10436
                     /locus_tag="ACHOQQ_RS07925"
                     /old_locus_tag="pgaptmp_001585"
     CDS             9318..10436
                     /locus_tag="ACHOQQ_RS07925"
                     /old_locus_tag="pgaptmp_001585"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGU4469851.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10502..10750
                     /locus_tag="ACHOQQ_RS07930"
                     /old_locus_tag="pgaptmp_001586"
     CDS             10502..10750
                     /locus_tag="ACHOQQ_RS07930"
                     /old_locus_tag="pgaptmp_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGU4469852.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            10815..11183
                     /locus_tag="ACHOQQ_RS07935"
                     /old_locus_tag="pgaptmp_001587"
     CDS             10815..11183
                     /locus_tag="ACHOQQ_RS07935"
                     /old_locus_tag="pgaptmp_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4469853.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11277..11930
                     /gene="nfsB"
                     /locus_tag="ACHOQQ_RS07940"
                     /old_locus_tag="pgaptmp_001588"
     CDS             11277..11930
                     /gene="nfsB"
                     /locus_tag="ACHOQQ_RS07940"
                     /old_locus_tag="pgaptmp_001588"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGU4469854.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12188..13435
                     /gene="ybdG"
                     /locus_tag="ACHOQQ_RS07945"
                     /old_locus_tag="pgaptmp_001589"
     CDS             12188..13435
                     /gene="ybdG"
                     /locus_tag="ACHOQQ_RS07945"
                     /old_locus_tag="pgaptmp_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGU4469855.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13503..14879)
                     /gene="pheP"
                     /locus_tag="ACHOQQ_RS07950"
                     /old_locus_tag="pgaptmp_001590"
     CDS             complement(13503..14879)
                     /gene="pheP"
                     /locus_tag="ACHOQQ_RS07950"
                     /old_locus_tag="pgaptmp_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGU4469856.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTIDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(14981..18124)
                     /gene="cusA"
                     /locus_tag="ACHOQQ_RS07955"
                     /old_locus_tag="pgaptmp_001591"
     CDS             complement(14981..18124)
                     /gene="cusA"
                     /locus_tag="ACHOQQ_RS07955"
                     /old_locus_tag="pgaptmp_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGU4469857.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDTTLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18136..19359)
                     /gene="cusB"
                     /locus_tag="ACHOQQ_RS07960"
                     /old_locus_tag="pgaptmp_001592"
     CDS             complement(18136..19359)
                     /gene="cusB"
                     /locus_tag="ACHOQQ_RS07960"
                     /old_locus_tag="pgaptmp_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGU4469858.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDRAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDSATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19375..19707)
                     /gene="cusF"
                     /locus_tag="ACHOQQ_RS07965"
                     /old_locus_tag="pgaptmp_001593"
     CDS             complement(19375..19707)
                     /gene="cusF"
                     /locus_tag="ACHOQQ_RS07965"
                     /old_locus_tag="pgaptmp_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGU4469859.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(19731..21113)
                     /gene="cusC"
                     /locus_tag="ACHOQQ_RS07970"
                     /old_locus_tag="pgaptmp_001594"
     CDS             complement(19731..21113)
                     /gene="cusC"
                     /locus_tag="ACHOQQ_RS07970"
                     /old_locus_tag="pgaptmp_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HGU4469860.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNGGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21270..21953
                     /gene="cusR"
                     /locus_tag="ACHOQQ_RS07975"
                     /old_locus_tag="pgaptmp_001595"
     CDS             21270..21953
                     /gene="cusR"
                     /locus_tag="ACHOQQ_RS07975"
                     /old_locus_tag="pgaptmp_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGU4469861.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            21943..23391
                     /gene="cusS"
                     /locus_tag="ACHOQQ_RS07980"
                     /old_locus_tag="pgaptmp_001596"
     CDS             21943..23391
                     /gene="cusS"
                     /locus_tag="ACHOQQ_RS07980"
                     /old_locus_tag="pgaptmp_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGU4469862.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLVMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEIGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVDHQVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIVLPERG"
BASE COUNT         5650 a         6408 c         5971 g         5429 t
ORIGIN      
        1 gcgttgcgcg aataatcggc ccaatttttt caaatgcccc tggagagata atatccacat
       61 gcgcacaatc ctgacgatag atatccagct ccgcaatcca cggcgaccag gcgcgttcag
      121 gactcatacc ttcctgaagc gtgcgttcag caacaaacaa cgtcgcttta ccgtcaaacg
      181 gtacgctatg agcagtcgtc agcaggcgca cagcatcagc gtagttgcct tcaatggtgg
      241 taaacaactc cgttgaagta cttccctgct gtgccgccag gaaggcctcg cgctcgcggt
      301 taatctccgc cagcacttcc gggtccagac cattagcttc tttttcctgc cagttttgcg
      361 tttctggcgg ccaggtatcc agcaagccaa gaaatgccac ctgttcgcca cgggcacgca
      421 gccgcgccgc aataccttgc gccagcgtac cgccaagtga ataacccagc aggtaataag
      481 ggccgtgcgg ttgttgttca agtaacgttg ccagatgcgc ttcgcagact tcatccaggt
      541 tcgtcgctgt ctgcatgggg ccatgagggc gcggcgactg aatgccgata atcgaccata
      601 gtggatcgag ataacgcgag agcacgctaa actgccaggc aaaaccggat gccggatgga
      661 aacaaaacag cgtcgggcca ttaccttcac gcaacggcag aatggtttcg aatcccatgc
      721 gccgggagct gtcctcttca ccatcaataa tcgttgccag tttggcgacg gttgacgcga
      781 ccatcacctg ccccggcgtc acctgacggg caaactgccg acttaactgc gctgccagtt
      841 tcattgccag tagcgaatga ccaccaagcg cgaagaaatc agcatcgaca tcctgcacgt
      901 cacaacccag taacgacgag aatgccgcgg cgataatcgt ttcactgccc gctttcggcg
      961 cacgccccgg cgtttgtgcc ttaagttcag gcaacggtaa ggctttgcga tccagcttgc
     1021 cgttggcgct aagaggtaac tgtggaagtt gcagcagaac gaccggcacc atatgcggcg
     1081 gcaatgtttc gcgaagctgt gcctgtaatg cgctggtatc caacggcaga cctgattgcg
     1141 acaccagata gcccaccaac tgacgcgcat caccaccggt ggctgccgcc tggttaatca
     1201 cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc ctgcatcacg cgatcgattt
     1261 cgcccagttc gatacgctgc ccgcgaattt ttagctgatc gtcactgcgc ccgaggtact
     1321 ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt acggtacatc cgttcgccag
     1381 gagcaaaagg atcggcaata aaacggctgg cggtcagatc gggtcgtcca agatacccct
     1441 gcgccagttg aatgccggtg agatagagat cacccgccac acccggcggc accggatgca
     1501 tcatcgcatc aagaatacgc agacccgtat tccataccgg ataaccaatc ggcacactgc
     1561 tgccgcgcac ctgtgccagt tcctcgccaa aagccggata ccagctgaca tctaccgccg
     1621 cttccgtcgg gccatataga ttatgcaacg gcgcgcccgt taactgttgc cattcgcggc
     1681 ataaatcagc cggtaaggcc tcaccactac agaaaacctg tttcaacgtc gcgcaactct
     1741 ggcgagcggt ttgcggagtc agcgatgcaa caaatgccgc cagcatcgac ggcacaaagt
     1801 gcgtggtcgt tacgccatat tcggcaaaga attgctgcat agcgagcggg tcgcggtgcg
     1861 cttccggttc agccatcacc agttttgccc cggcaataaa cggccagaaa aactcccaca
     1921 ccgagacatc aaaactgcac ggcgtttttt gggcaacgac atcttcgcct gtaagcgggt
     1981 agtgattttg catccataac aggcggttaa cgatagccgt ctgcccgacc attacccctt
     2041 tcggtctgcc ggtggaacca gaggtaaaga tgatataagc cgtgtggtgc ggttgtgaaa
     2101 gttgcagcgg cgcactgccc tgcggtgtaa gcggggcgtt atagcaaagg ttggttaaat
     2161 ctggaacatc ggcaaagcgc ggcagttgat catcggtggt gattaacagc gacggacgcg
     2221 catcttccag catcattttc aggcgatcgt ccggataccc cgtatccagc ggtaaccagg
     2281 ccgcacctgc ttcaacaatc gcatgtagcg ccagggtcaa aaagaccgag cgcggtaatg
     2341 ccaccgccac actgtcccct ggtttaacgc cgtgctcacg cagcagattc gccagcgcca
     2401 ccacctgctc gcgcatttcc cgatagctga actggtaacg cgcatctgcc agcgccggag
     2461 catccggtgt ttttgccgct tgttctgcca ccagcgcgct aagcgtggtt tctggaatct
     2521 caacctgagt ggcgttgatc tgcgccaact gcgcatactc acctggcagc ataatatcga
     2581 catcgccgca caacagcgcc ggatccgcgg cgaactgggc aatcagcatt ttcaggcgtt
     2641 cagaatgctg gattaacgtt ggctcatcgt aacgctgttt attggcaagg atctcaatac
     2701 tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag gtcattaacc ggcccggttg
     2761 ccagggtatg ggtttgcgcc tgaacaccag gaatgtccag ttggtaatca aatactttga
     2821 tattgagtac cggaccgaac agcggttcat cacctgccgc tcgcccactg tcacggacaa
     2881 tttgttcggc atcgtaacgt tgatggcgac gcattttttt cagttgtgct gccagtcggg
     2941 ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat acccaacggt aaaacgttga
     3001 gcacgggtcc ggtagccgtc agcgccgccg agcccagtcg acgcataaag ataaatccgg
     3061 cggcgtagtc catgcgattg cacaatcgcc ccagccacaa ggctgccagc gcaagggcta
     3121 aatcggtacg ctgcacacct gaaagttgcg cagccaactg gcggaattcc ccgtcggtaa
     3181 attccagttt caggcgcaga atatctgccg aggcgctacg ccccggtaaa ggtgccggag
     3241 aaagtgacgc gggcggtggc agttggcgac gctgttccac ccagaatgcc gcatcacgct
     3301 gccaggcttc gctttcgcgg tattgctggt actcttccac tacatcagcg aaaggcgtaa
     3361 acggcgaagc aggcgttggt tcgccacgca gcaatgcgca gtaaatgttg gcgatctggc
     3421 gggtaatggc cggaaaactg aagccatcga ccagtaaatg gtgataacgc tgataccagt
     3481 accagcggtt atctgccacc tgtatcagct ggtgaaagac cagtggttta ccgctatcga
     3541 cgcgcaaatc ttgttgcaaa tccgcctgca ttaatgcctg cgcagtaccg tgcggatcaa
     3601 tgttggttcg caggtcgata atttctggca gttcgaatat cagcgcatca tcgacccatt
     3661 gccagacttc gccgttatct tccgtaaaac gcatccgcaa cgtatctgct tgcgctaatc
     3721 cggcaaccac cgcgcgggcc agtaatggcg catcaacctc tccggttaac tcaacgtaat
     3781 gcgccacgct ccaggcggag ggtaaatccg acagtttttc tgccatccag atgccgggct
     3841 gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc tgcaactggg tgaaattcgc
     3901 cggtgtcaga gtacgccagt gggcttcaag ccactgctgg caggactctt gtgactgcgg
     3961 ctgacacaca atgtcccagc ctgccggtaa ggcgcattgt tgcggccaca gactgaattg
     4021 cccctgcgca ttgcgcaaaa tgtaaaacac tccctgtgga tcatcgaagg gattactgaa
     4081 tgccatattc aactcctgtc atggaaaagt ggttgccaga ggtcgattaa cccctgcatc
     4141 aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa cctgacgcca gaaaatggat
     4201 tcttttatag ggtgtaattg cgcatacagc gcctgattgg cccgcatgat catcggctcg
     4261 cgaatacccg cttccagcac aatgcgcaga ccttcggcgc taacttcacc agcttctagc
     4321 ttttcaagta acacgccctc ttgctgcccg ccccgatgcg gccaccagta cgatcctgac
     4381 tggcttaata cacagccaaa gcgttcaggc cagtgcagtc cggcatacag cgcggacagc
     4441 ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc gatcgctaaa aggggcaata
     4501 gctttcacca ggggcaataa ctcttgctgt actgccagcc agaaatccgc attacacggc
     4561 agttcgtggg cgcggtgcgt ggtgtcgata gcgtcgatca acacatacac ggcgggagga
     4621 agttgctgac gatgggtcag cgaagtcagc acaggccaga cgggcatact ttgcgcccaa
     4681 aattcgccat cgagcaaaac tgccagcggg cgttcttctg ctgttacatc gccggtggta
     4741 aaaatccata cacgacgtga atttttcaac cgttcacttt tccagataat ttctttggct
     4801 ggtatttctg gcgcttgcgg acaatcccat cccggttgca gaggcgcttg cggcatttcg
     4861 agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt gtggattcag cggatcggct
     4921 atcgcctggg gtaatagttt tcgccagcct tcacgtaatt cgaggcgatc gggggatggt
     4981 gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt agctgccgcg ccagttggca
     5041 ttgagttgtg tcgtccactg ccagacatca gtacctgcaa ttcgctgcat cgactggggc
     5101 tggctgttct gatgatgatc ggtcacacca gtgatgtaga cccatacacg ctttatcgtc
     5161 gagtattctt cagaaccttg gggatcacgc caccagaaag tgacctcaaa catttcgtca
     5221 ttcagacgct gccattccgg gccatgtttc gactgccacc agctctcact tcctactttt
     5281 aacgccgtca caccataacc ccatgtttac tgtgcaattt ttcattgatt gcagaaatat
     5341 attgataata ttattgataa ctatttgcat ttgcaatagc gtaatggcgc gccgtgggaa
     5401 gcgcggacat taattaacca actgcactgc gtgtctttca ggatcaaagg ttttcgcggt
     5461 agcgggatgc gtcgtgttga tgacgaccat gcccgacagt tgcaattcgt ggcaaaaatg
     5521 caggaataaa acaatgaaca agaagattca ttccctggcc ttgttggtca atctggggat
     5581 ttatggggta gcgcaggcac aagagccgac cgatactcct gtttcacatg acgataccat
     5641 tgtcgttacc gccgccgagc agaacttaca ggcgcctggc gtttcgacca tcaccgcaga
     5701 tgaaatccgc aaaaaccccg ttgcccgcga tgtgtcggag atcatccgta ccatgccagg
     5761 cgttaacctg accggtaact ccaccagtgg tcagcgaggg aataaccgac agattgatat
     5821 tcgcggtatg ggtccggaaa acacgctgat tttgattgac ggcaagccgg taagcagccg
     5881 taactcggtg cgtcagggct ggcgtggcga gcgcgatacc cgtggtgata cctcctgggt
     5941 gccacctgaa atgattgaac gtattgaagt tctgcgtggt ccggcagctg cgcgttatgg
     6001 caacggcgcg gcgggcggcg tggttaacat cattaccaaa aaaggcagcg gcgagtggca
     6061 cggctcctgg gacgcatatt tcaatgcgcc agaacataaa gaggaaggtg ccaccaaacg
     6121 cactaacttt agcctgaccg gtccgctggg cgacgaattc agcttccgct tgtatggcaa
     6181 cctcgacaaa actcaggctg acgcgtggga tatcaaccag ggccatcagt ccgcgcgtgc
     6241 cggaacgtat gccacgacgt taccagccgg gcgcgaaggg gtgatcaata aagatattaa
     6301 cggcgtggtg cgctgggact tcgcaccatt gcaatcgctg gaactggaag cgggttacag
     6361 ccgccagggt aacctgtatg cgggcgacac ccagaacacc aactccgatt cctatacccg
     6421 ctcgaaatat ggcgatgaaa ccaaccgtct gtatcgccag aactactcgc tgacctggaa
     6481 cggcggctgg gataacggcg tgaccaccag caactgggtg cagtacgaac acacccgtaa
     6541 ctcgcgtatt ccggaaggtc tggcgggcgg taccgaaggg aaatttaacg aaaaagcgac
     6601 acaggatttc gtcgatatcg atcttgatga cgtgatgctg cacagcgaag ttaacctgcc
     6661 gattgatttc ctcgttaacc aaacgctgac gctgggtacg gagtggaatc agcaacggat
     6721 gaaggactta agttccaaca cccaggcact gaccggaacg aataccggtg gcgctattga
     6781 tggcgtgagt gccaccgacc gtagcccgta ttcaaaagca gaaattttct cgctgtttgc
     6841 cgaaaacaac atggagctga ctgacagcac catcgtaacg ccggggctgc gtttcgatca
     6901 tcacagtatt gtcggcaata actggagccc ggcgctgaac atatcgcaag gtttaggcga
     6961 tgacttcacg ctcaaaatgg gcattgcccg tgcttataaa gcgccgagcc tgtaccagac
     7021 taacccgaac tacattctct acagtaaagg tcagggctgc tatgccagcg cgggcggctg
     7081 ctatctgcaa ggtaatgatg acctgaaagc ggaaaccagt atcaacaagg agattggtct
     7141 ggagttcaaa cgtgacggct ggctggcggg cgtgacctgg ttccgtaacg attatcgcaa
     7201 taagattgaa gcgggctatg tggctgtagg gcaaaacgca gccggcaccg atctctatca
     7261 gtgggataac gtgccgaaag cggtggttga aggtctggaa ggatcgttaa acgtaccggt
     7321 tagcgaaacg gtgatgtgga ctaataacat cacttatatg ctgaagagtg aaaacaaaac
     7381 cacgggcgac cgtttgtcga tcatcccgga gtatacgttg aactcaacgc tgagctggca
     7441 ggcacgagaa gatttgtcga tgcaaacgac cttcacctgg tacggtaagc agcagccgaa
     7501 gaagtacaac tataaaggtc agccagcggt tggaccggaa accaaagaaa ttagtcctta
     7561 cagcattgtt gggctgagcg cgacgtggga tgtgacgaag aatgtcagtc tgaccggcgg
     7621 cgtggacaat ctgttcgaca aacgtttgtg gcgtgcgggt aatgcccaga ccacgggcga
     7681 tttggcaggg gccaactata tcgccggtgc cggggcgtat acctataacg agccgggacg
     7741 tatacctata acgagccggg acgtacgtgg tatatgagcg taaacactca cttctgatgc
     7801 taacgtcaga ttgttgacaa agtgcgcgtc gttcatgccg gatgcggcgt gaacgcctta
     7861 tccggcctac aaaaatcttg ccaattcaat atattgcagg accatgtagg cctgataagc
     7921 gtagcgcacc aggcagtttt gcgtttgtca tcagtctcga atatggtcga tatgaaaact
     7981 acgcatacct ccctcccctt tgccgaacat acgctgcatt ttgttgagtt cgatccggcg
     8041 aatttttgtg agcaggattt actctggctg ccgcactacg cacaactgca acacgctgga
     8101 cgtaaacgta aaacagagca tttagccgga cggatcgctg ctgtttatgc cttgcgggaa
     8161 tatggctata aatgtgtgcc cgcaatcggc gagctacgcc aaccggtgtg gcctgcggag
     8221 gtatacggca gtattagcca ctgcggggct acggcattgg ccgtggtttc ccgtcaaccg
     8281 attggcgttg atatcgaaga aattttttct gcgcaaacag cgaccgaatt gacagacaac
     8341 attattacac cagcagaaca cgagcgactc gcagactgcg gtttagcctt ttctctggcg
     8401 ctgacactgg cattttccgc caaagagagc gcatttaagg caagtgagat ccaaactgat
     8461 gcaggttttc tggactatca gataattagc tggaataaac agcaggtcat cattcatcgt
     8521 gagaatgaga tgtttgctgt gcactggcag ataaaagaaa aaatagtcat aactctgtgc
     8581 caacacgatt aattgacaac atctggtacg attcgcccgc agccatcact gaccgcgggc
     8641 gaaagtgtaa agcaggtgcc ttaccatcct gacctgacaa ccggatatgc ggggattact
     8701 ccccgcacaa cggctacttc ttcggttcgt aagcgagaac agccctgaac tcaatattac
     8761 gttccttcac cgtaaactca cacagcgagt cgccgaccag aagcgtaaat cccagcaccg
     8821 tcaaacacag cactatgact gccacaaggg catatttcgt cagcatgttt atattgcctc
     8881 catgcgaaga agggactacc atccaaattg ttcaggtttg gagtggcagc cccagggttg
     8941 atagaaatat ctcctggggc ttcatctttc cagacctcag agttaacctg aaaccggaaa
     9001 gcttcaggca cccgccgtta tcttaccctt cctcaacaaa atggctgttt atttatacag
     9061 taaatcctgt ttcggacttc tcactcctca tttaatcaac tgaagctgag cccgtttttt
     9121 ctgcatgcct caatcgtcaa aattcttcgc caccagccag ccgtgccata acttgtcaca
     9181 ttgcaaaagt agccaaattg cttcaggcac catcagttca ccatcaccaa aaaaaagaaa
     9241 cattccagaa cttccgcatc tcgcccagcc gctccgactt ctatactgaa tagaaaacgc
     9301 caacataaga gaaacctatg ccattacccg attttcatgt ttctgaacct tttaccctcg
     9361 gtattgaact ggaaatgcag gtggttaatc cgccgggcta tgacttaagc caggactctt
     9421 caatgctgat tgacgcggtt aaaaataaga tcacggccgg agaggtaaag cacgatatca
     9481 ccgaaagtat gctggagctg gcgacggatg tttgccgtga tatcaaccag gctgccgggc
     9541 aattttcagc gatgcagaaa gtcgtattgc aggcagccgc agaccatcat ctggaaattt
     9601 gcggcggtgg cacgcacccg tttcagaaat ggcagcgtca ggaggtatgc gacaacgaac
     9661 gctatcaacg aacgctggaa aactttggct atctcatcca gcaggcgacc gtttttggtc
     9721 agcatgtcca tgttggctgt gccagtggcg atgacgccat ttatttgcta cacggcttgt
     9781 cacggtttgt gccgcacttt atcgcccttt ccgccgcgtc gccgtatatg cagggaacgg
     9841 atacgcgttt tgcctcctca cgaccaaata ttttttccgc ctttcccgat aatggcccga
     9901 tgccgtgggt cagtaactgg caacaatttg aagccctgtt tcgctgcctg agttacacca
     9961 cgatgatcga cagcattaaa gatctgcact gggatattcg ccccagtcct cattttggca
    10021 cggtggaggt tcgggtgatg gataccccgt taacccttag ccacgcggta aatatggcgg
    10081 gattaattca ggccaccgcc cactggttac tgacggaacg cccgtttaaa catcaggaaa
    10141 aagattacct gctgtataaa ttcaaccgtt tccaggcctg ccgctatggg ctggaaggcg
    10201 tcattaccga tccgcacacc ggcgatcgtc gaccgctaac ggaagacacc ttgcgattgc
    10261 tggaaaaaat cgccccttcc gcacataaaa ttggtgcatc gagcgcaatt gaagcactgc
    10321 atcgccaggt cgtcagcggt ctgaatgaag cgcagctgat gcgcgatttc gtcgccgatg
    10381 gcggctcgct gattgggctg gtgaaaaagc attgtgagat ctgggccggt gactaagtcg
    10441 ggaattgcct tacagaagaa tatccctgac aatggtgttt taacttacat ttaacaatgt
    10501 tatgaaacac cctttagaaa ccttgaccac cgcagcaggc attttgctga tggctttcct
    10561 ctcttgcctg ctgcttcccg cccccgcatt ggggctgacg ctggcacaaa aactggtgac
    10621 cacgttccat ctgatggatc ttagtcagct ttacacttta ttgttttgtc tgtggttttt
    10681 agtgctgggc gctattgagt attttgttct gcgctttatc tggcgacgct ggttctcgct
    10741 ggcggattaa acgtcgatta tcgccgtact tcgcggcata ctttgcttat tctccttcgc
    10801 ctaaaggaac gtttatggat aagcaatcac tgcacgaaac ggcgaaacgc ctggcccttg
    10861 agttaccctt tgtcgagctt tgctggcctt ttggcccgga gttcgatgtt tttaaaattg
    10921 gcggcaagat ttttatgctg tcgtcggagc tacgcggcgt cccctttatc aatctgaagt
    10981 ccgatccaca aaaatccctg ttaaatcagc aaatataccc aagcattaag ccagggtatc
    11041 acatgaataa aaagcactgg atttcggtgt atcccggcga ggaaatctcc gaagcgttac
    11101 ttcgcgatct gatcaacgat tcgtggaatc tggtggttga tggtctggct aaacgcgatc
    11161 aaaaaagagt gcgtccaggc taaagcggaa atctatagcg catttttctc gcttaccatt
    11221 tctcgttgaa ccttgtaatc tgctggcacg caaaattact ttcacatgga gtctttatgg
    11281 atatcatttc tgtcgcctta aagcgtcatt ccactaaggc atttgatgcc agcaaaaaac
    11341 ttaccccgga acaggccgag cagatcaaaa ctctcctgca atacagccca tccagcacca
    11401 actcccagcc gtggcatttt attgttgcca gcacggaaga aggtaaagcg cgtgttgcca
    11461 aatccgctgc cggtaattat gtgttcaacg aacgtaaaat acttgatgcc tcgcacgtcg
    11521 tggtgttctg tgcaaaaacc gcgatggacg atgcctggct gaagctggtt gttgaccagg
    11581 aagatgctga tggccgcttt gccacgccgg aagcgaaagc cgcgaacgat aaaggtcgca
    11641 agttcttcgc cgatatgcac cgtaaagatc tgcatgatga tgcagagtgg atggcaaaac
    11701 aggtttatct caacgtcggt aacttcctgc tcggcgtggc ggctctgggt ctggacgcgg
    11761 tacccatcga aggttttgac gccgccatcc tcgatgcaga atttggtctg aaagagaaag
    11821 gctacaccag tctggtggtt gttccggtag gtcatcacag cgttgaagat tttaacgcta
    11881 cgctgccgaa atctcgtctg ccgcaaaaca tcaccttaac cgaagtgtaa ttctcctgac
    11941 agccgggacc ccatcccggc tcagattgct gacaaagtgc gctttgttta tgccggatgc
    12001 ggcgtgaccg ccttatcctg cctacaagtt cgtgccaatt cattcaggta ggcatgataa
    12061 gcgtagcgca tcaggcaatt ttgtatttgc catcagccct cgcaggaaat cccccctcag
    12121 attctcctga tttgcataac cctgtttcag ccgtcatcat aggctgctgt tgtataaagg
    12181 agacgttatg caggatttaa tatcccaggt tgaagattta gcgggtattg agatcgatca
    12241 caccacctcg atggtgatga ttttcggtat tatttttctg accgccgtcg tggtgcatat
    12301 tattttgcat tgggtggtac tgcggacctt cgaaaaacgt gccatcgcca gttcacggct
    12361 ttggttgcaa atcattaccc agaataaact cttccaccgt ttagctttta ccctgcaggg
    12421 gattatcgtc aatattcagg cggtattctg gctgcaaaaa ggcaccgaag cggcagatat
    12481 tctgactacc tgcgcgcagt tgtggatcat gatgtatgcg ctgctttcag tcttctcgct
    12541 gctggatgtt attttgaatc tggcgcagaa attcccggca gcatctcagt taccgctgaa
    12601 agggatattt caagggatta aactgatcgg cgcgattctg gtcggcattt tgatgatctc
    12661 gctgctgatt ggtcagtcgc cagcgattct gatcagcggt cttggtgcaa tggctgccgt
    12721 gctgatgttg gtatttaaag atccgattct tggtctggtg gcaggtattc agctttccgc
    12781 gaacgatatg ctgaaactgg gcgactggct ggagatgccg aaatacggcg cggatggcgc
    12841 ggtgatcgat attgggttaa ccaccgtcaa agtgcgtaac tgggacaata ccattaccac
    12901 tattcccacc tggtctctgg tttctgactc ctttaaaaac tggagcggga tgtcagcatc
    12961 cggcgggcga cgtattaagc gcagtatcag tattgatgtc accagtattc gttttcttga
    13021 tgaagacgaa atgcaacgtc tgaataaagc gcatttgtta aagccttatt taaccagccg
    13081 ccatcaggaa attaatgagt ggaatcgcca gcaaggttct acggagtcgg tattaaacct
    13141 gcgccgaatg accaatattg gaacctttcg tgcctatctg aacgaatatc tgcgtaacca
    13201 tccgcggatt cgtaaagata tgaccttaat ggtacgccaa ctggctccag gtgataacgg
    13261 tttaccgctt gagatctatg cgtttaccaa caccgtggtg tggctggaat atgaaagcat
    13321 tcaggctgat atattcgatc acatatttgc gattgtcgaa gagtttggtc tgcgacttca
    13381 tcagtcgcca accggcaatg atattcgctc tctggcgggt gcatttaagc agtaattaaa
    13441 aaaaccgctc tcatcgaatg gatgagagcg gacatcgttt tgtcggatgt agcgtgaatg
    13501 ccttatttcc gacgcagcgt tttaaatgcc acaaacagga atacaatcca caccggcagc
    13561 aggatcgctg acaagcgcat atcatctatc gtgcacatca gcagcaaaat catgccgagg
    13621 aaggcaatgc agagatagtt tccgaacgga tagagcagcg ccttaaactg tgtttcacgc
    13681 ccctgacgtc gcatcgctgc acgaaaacgc agatgcgcca gacagatcat aatccagttc
    13741 aacagcagcg ttgctaccac cagcgccatc agcagaccaa acgctttttg cggcagcaga
    13801 tagttgatta acaccaccag cgaagtgatc gctccggaaa gcatcagcga gttaatcggc
    13861 acgccgcgac ggctgacgcg agtcaaaaac ttcggcgcat taccctgtac agaaaggcca
    13921 aacagcatgc ggctgttaga gtaaaccccg ctgttataca ctgacagcga tgctaccaga
    13981 atgacgaagt tcagcgcaga agctaccacg ttgctgtcga gattatggaa aatcatcaca
    14041 aacgggctac tgttggattt cacttccacc cacggataga gcgccagtaa aaccaccagt
    14101 gaaccgatgt aaaacagcag gatgcgatac accacctgat ttaccgcttt tggaatgctt
    14161 ttttccggat cgcgcgcttc agcggcagta atcccaatca gctccagacc gccgaaggag
    14221 aacataatta ccgccagcga caaaatcagc ccattccagc cggtggcgaa gaaaccaccg
    14281 tagcgccaga ggttgtcgat actggctttc tcgccgccgt gaccagaaaa cagcagccac
    14341 aggccaaagc cgatcatacc gatgattgcc agcactttaa tcagcgcaaa ccagaactcg
    14401 gtttcgccat ataagcgcac gttcaccagg ttaacggcgt tgatgataat aaagaaggcg
    14461 gcagcccaaa tccacgttgg aacatccggg aaccagtact gcatatagat gcccgcagcg
    14521 gtcagctctg ccattcccac cagcacgaac atcacccagt aattccagcc agagaggaag
    14581 cccgcgaacg gtccccagta tttataggca aagtgggcaa atgaaccgga taccggctcc
    14641 tcaaccacca tttcgccaag ctggcgcata atcaggaaag cgatgatccc ggcgacgccg
    14701 tagcccagca atacagccgg acccgccatc tgaatcgccg ggccaatgcc aagaaacaga
    14761 ccagtaccaa ttgcgccacc cagcgcaatc agttgaatat gacggttatg taatccgcga
    14821 tgaagcgtcg gctcttgatt cgacgcagta tcttccgata cggttgacgc gtttttcacg
    14881 catttcccct gtgtgtcttt tttgttgagg ggcacctttt aacatttagt gcccatcgtc
    14941 gcaagacaca atccacacgg ttaaacgggg tatcctgctt ttatttccgt acccgatgtc
    15001 ggtgcagcca catcagctta tacgccgccg ggataataaa cagcgacagc aaaggcgcgg
    15061 tgatcatgcc gccaatcatc ggcgcggcaa tccggctcat cacctctgac cctgctcctg
    15121 ttccccacag aatcggcaac agaccagcga taatcaccgc caccgtcatc gctttcgggc
    15181 gcacgcgcag gaccgcgccg tgatacaacg cctcatccag cttctgctcg ctgaatgttt
    15241 gcggattatt caacgacggc tcggcctcta tggcgtgacg taaatacatc agcatcacca
    15301 cgccaaattc ggcggcgacc ccggcaaggg caataaaacc ggtgcccgtc gccacagaaa
    15361 gatgaaagcc catccaccac agcaaccaga tgccgcccac cagcgcaaac ggtacgctgc
    15421 tgataatcag caacgcttcg cccacccgac ggaatgccag atacaacagc acgaagatga
    15481 tcatcagtgt catcggcacc atgagtttca gcttatggtt ggcgcgctcc agtagctcga
    15541 actgcccgga gaatgccacg ctggtgcccg gttttaactg cactttttca gctatcgctt
    15601 tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg ggcatcgata taaatccagc
    15661 tcgtcgggcg cgcattctcg gttttcagca ttgacggtcc ggtagagact ttaacgtcgg
    15721 ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag gatcggcaac tggcgcagtg
    15781 cctgcggact atcgcgccag ctttgcggat aacgcagatt aattggataa cgggcaatcc
    15841 cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga agtcacaaac aactgcacat
    15901 ccgccaccgt cataccgtaa cgcgcggctt tttcacggtt aatctcaacg ttgatatagc
    15961 gcccaccttc cagacgctcg gcaagcgcag aagctacgcc tggcaccgtt cgcgccactt
    16021 cttcaatttg ctccgccatc gcgtcgatat ccgccagcac agtgccggaa actttaatgc
    16081 cgatggggct tttaatgccg gttgagagca tatcgatacg gttacgaatt ggcggcaccc
    16141 acagattcgc cagccccggc agacgcacgg tgttatccag ttcctcaatg attttgtcca
    16201 ttgtcatgcc tggccgccac tgatcctgcg gcttaagctg gatggtcgtt tctaccatct
    16261 ccagcggagc agaatcggtg gcggtttccg ctttcccggt tttgccaaat acccgcgcca
    16321 cttcaggtac gctcataatc agcttgtcgg ttttttgcag catactcgcc gcctctgctg
    16381 cggaaatccc cggcagcgtc gatggcatat acaacaagtc gccttcattg atctgcggta
    16441 aaaattcccc gccaacttta ttgagcggcc agagaaccgt cagcaccgaa agcgctgcca
    16501 ccaacagcgt ggttttcggc cagtgcagta ctttcagcaa tagaggatga taaacacgaa
    16561 tcaaaaagcg attgagcggg ttactgcttt ccggcggaat tttgccacgg atccagtaac
    16621 ccatcaggat agggatcact acgatcgcca gcagcgccgc acccgccatc gcatacgttt
    16681 tggtgaacgc cagcgggcca aacagacgcc cttcctgccc ttccagggtg aagatcggga
    16741 taaacgacag tgtgataatc agcagactga taaacagcgc cggtcccact tcaacagacg
    16801 catcggtaat cacctgccag cgcgttttat tatccagcgt ggtgtcagga tgctggtgct
    16861 gccactcttc cagccgttta tgcgcattct cgatcatgac gatagcggca tcgaccatcg
    16921 ccccgacggc aatcgcaata ccgcccagcg acataatatt ggcattcagc ccctggaagt
    16981 gcatgacaat aaaagcaata cacaacccca gcggcaacga aataatcgcc accagcgccg
    17041 agcgcacatg ccagagaaac agcgcacaga ctaccgccac cacaataaac tcttccagca
    17101 acttgccgct gagattgtcg atggcccggt caatgagctg gctgcgatcg tatgttgtaa
    17161 ctatctccac gccttccggc agactgcttt tcagcgtttc tagtttgtcc ttcacggcgg
    17221 cgatcacttc acgggcgttt ttgccggagc gtaggatcac caccccgccc gcaacttcgc
    17281 cttcgccgtt cagttcggca atgccccggc gcatctccgg gccaacctgg atcttcgcaa
    17341 catcacgcag ataaacgggc acgccatttt cactggcttt taaaacgatg tgattaaagt
    17401 cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata ttctgcttcc gccagttcga
    17461 ttgacgaacc gcccgcttcc tggtttgaag catccagcgc gcttttaact tcggcgagac
    17521 tgatgccata ctgcgccagg cgctggggat cgataaccac ctgatactct ttcaccacgc
    17581 cgcccaccga cgccacttcc gcaacgtcag ggatggtttt tagctcatat ttgagaaacc
    17641 agtcctgcaa tgagcgtaaa tcggccagat cgtgcttacc gctgcgatcc accagtgcat
    17701 attcatagat ccagccaaca cccgtggcat ctggccccag ctcggcgctg actcccgcag
    17761 gcagcttacc ctgtacctgg ttgaggtact ccagcacccg cgagcgcgcc cagtacggat
    17821 cggtgccatc ttcgaaaatg acatacacat aagagtcgcc aaactgcgag aaaccgcgca
    17881 cagtcttcgc gccaggtacc gacaacatgg tggtagttag cggataagtc acctgatttt
    17941 caacgatttg cggtgcctgc ccgggatagc tggttttaat aatcacctgc acatcggaga
    18001 gatccggcaa cgcatccact ggcgtgttaa tgatggtcca ggtgccccag atactcagaa
    18061 ataacgcgcc catcagcacc agaaaacggt tcgccaccga gcgacgaata atccattcaa
    18121 tcattggtta ttccctcaat gcgcatgggt agcactttca gagcgcatcc gctccagtgc
    18181 gccagaaata ttggcttcgg aatcaatcag gaacaggccg ctggaaacca ccttttcacc
    18241 ttccgccaga ccagagcgta acgcggtgac accttgcgat gcctggaaaa cggcaacgcg
    18301 tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc cgctgttcgt tgccggtatc
    18361 aatcagcgct tgtgacggaa tgagcagcat cggttcgctg gcggtattga gttgcaacca
    18421 ggcgttcatt cccggtttta gcgcctcgtc ggcgttgtcg acttccagac gcagctgcag
    18481 cgtgcgggtc gcggaatcca cgccaggtag tagcgtccat ttgcggatgg tgagtgtttt
    18541 atcaggtcgc gccggaacgg tgagcgtaaa ctgcgaggca tctttcacca gccaggcgat
    18601 ggactccggg atcgcagcag tgacccacac cgggtccata ccctgaattt tcgcgaccac
    18661 gttatctttg gcgatattca ttcccgcgcg cagatcaaac gcggtgatca cgccatcaat
    18721 gggtgctttg agcgtaaagc gagtctggat tttttgcgtg gcgatcaggc ggcgaatatc
    18781 cgcctccggc attcccgcca gtcgcagccg ctcaagaatg ccttcagtct gggtcgccgt
    18841 accgccggtt tcgcgcagca gtaaatactc actctgcgct tccacccagt cgggaatggt
    18901 caggtcgaga agcggtgtgc ccttttgcac tttatcgccc acggtaagcg gatacacctt
    18961 gtcgataaac ccggcagcgc gagcctgcac aatggcatac tgatactcgt tgtaactgac
    19021 attcgccggg aaactctggg caaaagtcag cggtccgcgc gtgacggtag ccgttttcac
    19081 ccccagattc tgcgtctgag tcgggtcaat gcgcacacca gacgcagaac tctcttcatc
    19141 ggcatatttc ggcaccagat ccatatccat aaacggcgat ttacctggtt tatcgaaccg
    19201 cgtattggga tacatcgggt cgtaccagaa taagatttta cgttctgcag tcgacgtttt
    19261 ttctgcgggc ggttcagccc ggtcaaacca ggtaaaacct gccgcagaaa taataccgcc
    19321 cgcgatcatg ctgccgataa taagcgcgat ttttttcatc tcattaaacc tgggttactg
    19381 gctgacttta atatcctgta ataaagaaag gttgccctgc tggacaaaat taaacgccac
    19441 tttgtcgcca gttttaattt cactcatttt cgtctgcgga gtgatggtaa agcgcatagt
    19501 catctccggc cagttcacgg cagcaatcgg atcgtgatgg atggtgattt ttttgctttc
    19561 cagatcaaca ccttttacca cgccggtggc actaatgacc tgtggttgtg cttcgctcat
    19621 ggtttcatga tgatgttcgt tagcctgggc attaaagcca ataacggtaa acagactgaa
    19681 cattgcgact tgcagtgctt ttttcattta ctctctcctg gagttaaaaa ttattgctgc
    19741 caaccgccac caagtgcggt atataaagaa atttcgttga cctgacgggc ataattcagg
    19801 tcaagtaagg tttgtcgggt tgcaaataaa gaacgctcgg catccagcac ttccagataa
    19861 cttaccgcgc cgtgctgata taatgcccgc gcccgttgca aagtaatttg cagcgacgcc
    19921 agataacgct gctgggcgct gatttgatcg ttcaggcttt gacgtaatgc cagcgcatct
    19981 gccacttctt taaaggcgtt ctggattttc tgttcataat tcactaccga ctgctgctgg
    20041 cgaatttcgg cgatatccag attggcctgg ttgcgtccgg cattaaaaat ggggatctca
    20101 attttgggta taaaattcca catcccgctg ctggcgttaa ataatgacga cagatcgctg
    20161 ctggcggttg atattccgct ggtcaggctg atagacggga aaaatgccgc gcgtgcagca
    20221 ccaatattgg cattagccgc cattaacgca tgttcagctt ccataatatc cgggcgctgc
    20281 aataagattt gcgacgacaa gcccgccggt aatttaacgc tttgcagact gccgccgttt
    20341 actgtctgcg cttgcggcag cttgccgtag cttcccaata acagttgcaa tgcattattc
    20401 gcctgcgcca gttccccctg acgtttagcg atgtcgctgc gggtactttc aatcaccccg
    20461 cgagcctgtt ccagcgccag aacattactg ctacctgtca acagttgttt ttcgacaaac
    20521 gcatatgact gctgataatt acgcagcgtt tcttcggcta tttgcaattg cgcatacgcc
    20581 agctgctgat tgaaatagct ttgcgcgaca ttagaaacca gcagaatatg caccgcgcgc
    20641 tgagcttcct cagtggctaa ataattttgt cgctcggctt cgctcatgtt ctttaagcgc
    20701 ccgaaaaaat cgaggtcaaa gctggcgtta aggccagtag aaaactcccg cgtcgtggct
    20761 gaatcgcctt taagattgcc gctccagctg ccgctgccct caccattgag ctgtgggtag
    20821 cggtcggcat cagtcagacg atattgcgcc cgtgcctcct gcactttcag cgtcgccatg
    20881 cgcaaatccc ggttattctc cagcgcctcg ctaatcagcg tcttcacctg attatcaaca
    20941 aaaaaggtgc gccagcccgc gttctgatag ttatctgctg cgttaaccag gccgttctgg
    21001 ctgagtgaga actgctgagg aacgggcatt gccggacgct gataatccgg tgccagtgaa
    21061 caaccggtta gcgcaagggc cacacaaaat ggcagaagtt tacaaggaga cataggctca
    21121 taatttctgg tgattttata ccgccaactt tactcgccag gctctgattt tccggtgaca
    21181 ggaaaatgac aaaattgtca ttttgccaat aagcgattgc catctgatcc cgctactcta
    21241 gaattgcccg ggcaacatgc ggaggaaata tgaaactgtt gattgtcgaa gatgaaaaga
    21301 aaaccggaga atacttgacc aaagggttaa ccgaagccgg ttttgtggtc gatttggccg
    21361 acaacgggct gaatggctac catctggcga tgaccggtga ttatgatctg ataatcctcg
    21421 atattatgct gccggacgtg aacggctggg atatcgtgcg catgttacgc tccgccaata
    21481 aagggatgcc gattctgttg cttaccgcgc ttggcaccat tgaacatcgc gtcaaggggc
    21541 tggagttggg ggcagatgac tacctggtga aaccattcgc ttttgctgaa ctactggcgc
    21601 gggtgcgcac attactgcgg cgcggggcgg cggtgattat cgaaagtcag tttcaggttg
    21661 ccgatttgat ggtcgatctc gtcagccgca aagtcacccg cagcggcacg cgcatcactt
    21721 tgaccagtaa agagtttact ctgctggagt tttttctccg ccatcagggc gaagtgctgc
    21781 cccgctcgct tatcgcctcg caggtatggg acatgaattt tgacagcgac actaacgcca
    21841 ttgatgtggc ggtgaagcgg ctgcgcggca aaatcgacaa cgactttgag ccgaagctaa
    21901 ttcagaccgt gcgcggcgtg ggttacatgc ttgaggtgcc ggatggtcag taagccattt
    21961 cagcgcccgt tttcgctggc aacccgcctg acctttttta tcagcctggc caccatcgcg
    22021 gcgtttttcg cctttgcatg gatcatgatc cactcagtaa aagtgcattt tgccgagcag
    22081 gatattaatg atttaaaaga gattagcgcc acccttgaac gcgtactaaa tcaccctgac
    22141 gaaacgcaag cccgacgctt aatgacgctg gaagatatcg tcagtggtta ttccaacgtg
    22201 ttgatttccc tggcagatag ccacggtaaa acggtgtatc actcccccgg tgcgccggat
    22261 atccgcgagt ttacgcgtga cgccataccc gataaagacg ctcagggtgg cgaggtgtat
    22321 ctcctttccg gcccgacgat gatgatgcca ggccacggtc acgggcatat ggaacacagc
    22381 aactggcgga tgatcagttt gccagttggc ccactggttg acggcaaacc gatttatacg
    22441 ctctacatcg cgctttcgat cgattttcat cttcattaca taaatgattt gatgaataaa
    22501 cttgtaatga ccgcatcgat catcagcatc ctgattgtct ttatcgtgct attggcggta
    22561 cataaaggtc acgcgccgat tcgcagcgtc agccgtcaaa tccagaatat tacctcgaaa
    22621 gatctcgacg ttcgcctcga cccgcagacc gtgccgattg agctggaaca gctggtactg
    22681 tcgttcaacc atatgatcga gcgtattgag gatgtcttta cccgccagtc caatttctca
    22741 gcggatatcg ctcacgaaat tcgtacgccg attaccaatc tcatcacgca aacggaaatc
    22801 gccctcagcc agtctcgcag ccagaaggag ctggaagatg tgctctactc taatctcgaa
    22861 gagctgacgc gaatggcgaa aatggtcagc gatatgctgt ttctcgccca ggccgataac
    22921 aaccagctaa tccccgaaaa gaaaatgctc aacctggcgg atgaaatcgg caaagtgttc
    22981 gattttttcg aggcgttagc ggaagatcgc ggcgtggagt tgcggtttgt tggcgacaaa
    23041 tgtcaggttg cgggcgatcc gctgatgctg cgtcgggcgt taagcaacct gctttctaat
    23101 gccctgcgtt atacgccacc cagtgaggct attgtagtgc gctgccagac ggtcgatcac
    23161 caggtgcaag ttatcgtcga aaaccccggt actcccattg cgcccgagca cttaccgcga
    23221 ttgtttgacc gtttctaccg cgttgaccct tcccgccagc gaaaaggcga aggtagcggc
    23281 attgggctgg cgatagtgaa atcgattgtt gtcgcacata aaggcacggt tgcagtaacg
    23341 tcagatgcgc gggggacaag gtttgtgatt gtgttgccgg agagggggtg attattaagt
    23401 tcaaattcag ggcgagggtt tacagcgttt ttcgctaaat ttccatcatt ataaacaa
//
LOCUS       DAZKFU020000051        23370 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZKFU020000051 DAZKFU020000000
VERSION     DAZKFU020000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.051"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..241
                     /locus_tag="ACHOQQ_RS07985"
                     /old_locus_tag="pgaptmp_001597"
     CDS             <1..241
                     /locus_tag="ACHOQQ_RS07985"
                     /old_locus_tag="pgaptmp_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HGU4469863.1"
                     /translation="HADYPGNEYLFGKQIAESELLWPVDRVQKSLNGELQQINGADYF
                     IFGHMMLDNIQTFANQIYIDTGSPKSGRLSFYKIK"
     gene            complement(282..518)
                     /locus_tag="ACHOQQ_RS07990"
                     /old_locus_tag="pgaptmp_001598"
     CDS             complement(282..518)
                     /locus_tag="ACHOQQ_RS07990"
                     /old_locus_tag="pgaptmp_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HGU4469864.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(529..1956)
                     /locus_tag="ACHOQQ_RS07995"
                     /old_locus_tag="pgaptmp_001599"
     CDS             complement(529..1956)
                     /locus_tag="ACHOQQ_RS07995"
                     /old_locus_tag="pgaptmp_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HGU4469865.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESSYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(1956..2549)
                     /locus_tag="ACHOQQ_RS08000"
                     /old_locus_tag="pgaptmp_001600"
     CDS             complement(1956..2549)
                     /locus_tag="ACHOQQ_RS08000"
                     /old_locus_tag="pgaptmp_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HGU4469866.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            2696..3103
                     /locus_tag="ACHOQQ_RS08005"
                     /old_locus_tag="pgaptmp_001601"
     CDS             2696..3103
                     /locus_tag="ACHOQQ_RS08005"
                     /old_locus_tag="pgaptmp_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGU4469867.1"
                     /translation="MALRNKAFHQLRQLFQQHTSRWQHELPGLTKPQYAVMRAIADKP
                     GIEQVALMEAAVSTKATLAEMLARMENRGLVRRDHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(3223..4215)
                     /gene="rpoS"
                     /locus_tag="ACHOQQ_RS08010"
                     /old_locus_tag="pgaptmp_001602"
     CDS             complement(3223..4215)
                     /gene="rpoS"
                     /locus_tag="ACHOQQ_RS08010"
                     /old_locus_tag="pgaptmp_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HGU4469868.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(4278..5366)
                     /gene="nlpD"
                     /locus_tag="ACHOQQ_RS08015"
                     /old_locus_tag="pgaptmp_001603"
     CDS             complement(4278..5366)
                     /gene="nlpD"
                     /locus_tag="ACHOQQ_RS08015"
                     /old_locus_tag="pgaptmp_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGU4469869.1"
                     /translation="MVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGT
                     TSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYS
                     GSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGN
                     AITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAP
                     VTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAII
                     ATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGS
                     TGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(5557..6183)
                     /gene="pcm"
                     /locus_tag="ACHOQQ_RS08020"
                     /old_locus_tag="pgaptmp_001604"
     CDS             complement(5557..6183)
                     /gene="pcm"
                     /locus_tag="ACHOQQ_RS08020"
                     /old_locus_tag="pgaptmp_001604"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGU4469870.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(6177..6938)
                     /gene="surE"
                     /locus_tag="ACHOQQ_RS08025"
                     /old_locus_tag="pgaptmp_001605"
     CDS             complement(6177..6938)
                     /gene="surE"
                     /locus_tag="ACHOQQ_RS08025"
                     /old_locus_tag="pgaptmp_001605"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGU4469871.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPAEQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(6919..7968)
                     /gene="truD"
                     /locus_tag="ACHOQQ_RS08030"
                     /old_locus_tag="pgaptmp_001606"
     CDS             complement(6919..7968)
                     /gene="truD"
                     /locus_tag="ACHOQQ_RS08030"
                     /old_locus_tag="pgaptmp_001606"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGU4469872.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(7965..8444)
                     /gene="ispF"
                     /locus_tag="ACHOQQ_RS08035"
                     /old_locus_tag="pgaptmp_001607"
     CDS             complement(7965..8444)
                     /gene="ispF"
                     /locus_tag="ACHOQQ_RS08035"
                     /old_locus_tag="pgaptmp_001607"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGU4469873.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(8444..9154)
                     /gene="ispD"
                     /locus_tag="ACHOQQ_RS08040"
                     /old_locus_tag="pgaptmp_001608"
     CDS             complement(8444..9154)
                     /gene="ispD"
                     /locus_tag="ACHOQQ_RS08040"
                     /old_locus_tag="pgaptmp_001608"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGU4469874.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(9173..9484)
                     /gene="ftsB"
                     /locus_tag="ACHOQQ_RS08045"
                     /old_locus_tag="pgaptmp_001609"
     CDS             complement(9173..9484)
                     /gene="ftsB"
                     /locus_tag="ACHOQQ_RS08045"
                     /old_locus_tag="pgaptmp_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGU4469875.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(9678..10001)
                     /gene="ygbE"
                     /locus_tag="ACHOQQ_RS08050"
                     /old_locus_tag="pgaptmp_001610"
     CDS             complement(9678..10001)
                     /gene="ygbE"
                     /locus_tag="ACHOQQ_RS08050"
                     /old_locus_tag="pgaptmp_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGU4469876.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(10051..10656)
                     /gene="cysC"
                     /locus_tag="ACHOQQ_RS08055"
                     /old_locus_tag="pgaptmp_001611"
     CDS             complement(10051..10656)
                     /gene="cysC"
                     /locus_tag="ACHOQQ_RS08055"
                     /old_locus_tag="pgaptmp_001611"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGU4469877.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(10656..12083)
                     /gene="cysN"
                     /locus_tag="ACHOQQ_RS08060"
                     /old_locus_tag="pgaptmp_001612"
     CDS             complement(10656..12083)
                     /gene="cysN"
                     /locus_tag="ACHOQQ_RS08060"
                     /old_locus_tag="pgaptmp_001612"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGU4469878.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(12085..12993)
                     /gene="cysD"
                     /locus_tag="ACHOQQ_RS08065"
                     /old_locus_tag="pgaptmp_001613"
     CDS             complement(12085..12993)
                     /gene="cysD"
                     /locus_tag="ACHOQQ_RS08065"
                     /old_locus_tag="pgaptmp_001613"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGU4469879.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            13245..14282
                     /gene="iap"
                     /locus_tag="ACHOQQ_RS08070"
                     /old_locus_tag="pgaptmp_001614"
     CDS             13245..14282
                     /gene="iap"
                     /locus_tag="ACHOQQ_RS08070"
                     /old_locus_tag="pgaptmp_001614"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGU4469880.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   14303..14819
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=14364..14392
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(14916..15209)
                     /gene="cas2e"
                     /locus_tag="ACHOQQ_RS08075"
                     /old_locus_tag="pgaptmp_001615"
     CDS             complement(14916..15209)
                     /gene="cas2e"
                     /locus_tag="ACHOQQ_RS08075"
                     /old_locus_tag="pgaptmp_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HGU4469881.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(15206..16129)
                     /gene="cas1e"
                     /locus_tag="ACHOQQ_RS08080"
                     /old_locus_tag="pgaptmp_001616"
     CDS             complement(15206..16129)
                     /gene="cas1e"
                     /locus_tag="ACHOQQ_RS08080"
                     /old_locus_tag="pgaptmp_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HGU4469882.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGYIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(16126..16776)
                     /gene="cas6e"
                     /locus_tag="ACHOQQ_RS08085"
                     /old_locus_tag="pgaptmp_001617"
     CDS             complement(16126..16776)
                     /gene="cas6e"
                     /locus_tag="ACHOQQ_RS08085"
                     /old_locus_tag="pgaptmp_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HGU4469883.1"
                     /translation="MYLSRITLHTAQLLPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGRDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(16758..17504)
                     /gene="cas5e"
                     /locus_tag="ACHOQQ_RS08090"
                     /old_locus_tag="pgaptmp_001618"
     CDS             complement(16758..17504)
                     /gene="cas5e"
                     /locus_tag="ACHOQQ_RS08090"
                     /old_locus_tag="pgaptmp_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HGU4469884.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQGGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDHFHDLKVSLPKLQDECWWEGEHDGLVVSKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(17515..18570)
                     /gene="cas7e"
                     /locus_tag="ACHOQQ_RS08095"
                     /old_locus_tag="pgaptmp_001619"
     CDS             complement(17515..18570)
                     /gene="cas7e"
                     /locus_tag="ACHOQQ_RS08095"
                     /old_locus_tag="pgaptmp_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HGU4469885.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(18585..19121)
                     /gene="casB"
                     /locus_tag="ACHOQQ_RS08100"
                     /old_locus_tag="pgaptmp_001620"
                     /gene_synonym="cse2"
     CDS             complement(18585..19121)
                     /gene="casB"
                     /locus_tag="ACHOQQ_RS08100"
                     /old_locus_tag="pgaptmp_001620"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HGU4469886.1"
                     /translation="MSIVKEEHKATLRKWHEELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLAEDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(19118..20680)
                     /gene="casA"
                     /locus_tag="ACHOQQ_RS08105"
                     /old_locus_tag="pgaptmp_001621"
                     /gene_synonym="cse1"
     CDS             complement(19118..20680)
                     /gene="casA"
                     /locus_tag="ACHOQQ_RS08105"
                     /old_locus_tag="pgaptmp_001621"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HGU4469887.1"
                     /translation="MNSFSLLTTPWLPVRYKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPDVPGAQTTKFNKDHFIKRGVTEYLCPHCLALALFSLQLNA
                     PAGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWLNVMPQDEADLPLPQKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDPDELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQNA
                     KAAREKKQEAAE"
     gene            complement(20777..>23370)
                     /gene="cas3"
                     /locus_tag="ACHOQQ_RS08110"
                     /old_locus_tag="pgaptmp_001622"
     CDS             complement(20777..>23370)
                     /gene="cas3"
                     /locus_tag="ACHOQQ_RS08110"
                     /old_locus_tag="pgaptmp_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HGU4469888.1"
                     /translation="LLCWHSLDVAAMGYLMVKRNCFGLADYFYQLGISDKEQAAQFFA
                     WSLCWHDIGKFAHSFQQLYLPPELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELL
                     PSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELDNFLPEDKAAARDFLLAIKALFP
                     RIEIPAFWDDDEGIELIKQLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTL
                     VQAQNALTVFPLKAKVAPFTGINTLFPFIENPTPLQQKVLDLDISQPGPQLFILEDVT
                     GAGKTEAALILAHRLMAAGKAQGLFFGLPTMATANTMYDRLVKTWLSFYSPESRPSLV
                     LAHSARTLMDRFNESLWSGDLIGSEEPDEQAFSQGCAAWFADSNKKALLAEIGVGTLD
                     QAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLS
                     ATLSQQQRDKLVTAFARGTEGQQEAPLLEKDDYPWLTHVTQTDVNSHRVATRKDVERS
                     VSVGWLHSEQECIARIESSVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFH
                     SRFAFSDRQRIEMETLARFGKEDGSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDL
                     LIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGRAMRNSAFV
                     YPDHGRIWLTQRVLRGQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCD
                     RTMAKKFVLNFRPGYAASINDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAW
                     EMSVVRVRRSWWKKHRDEFSLLEGDAFRQWCIEQRQEPEMANVILVTDDESCGYSAME
                     GLTGKVG"
BASE COUNT         5542 a         6424 c         5778 g         5626 t
ORIGIN      
        1 acatgcggat tatccgggca atgaatatct ctttggcaaa caaatagcgg agagcgaatt
       61 actttggcct gttgatcgtg tgcagaaatc tcttaatggc gagttacaac aaataaacgg
      121 cgctgattat tttatatttg gacatatgat gcttgataac attcagacgt tcgctaacca
      181 gatttatatt gataccggat cgccgaaaag cgggcggctg tcattttata aaataaagta
      241 atgaggccgg gtaaagcaaa gccactaccc ggcacttttt attagcgctt accttccgcc
      301 agcagcggcg ggatgctcgg caccattggc gcgtcatcaa tatctttctg cgtcatgcgg
      361 aacgcttcgg gataatgttc gcggctggta cggcgcagcg gttcggtatc gcgccaggta
      421 taaaggcaat gctggcactg atataccgtc cagacatctt tcaccggcga tttcgccatc
      481 acttcaatct gttcatcggc acaacgtgga caaatcatct tgttctcctt atttacgtgc
      541 ggccagcata gcggtcagtt tttcagccca ggctttggtt tccggcaaat ccaccaccgg
      601 ctggctgtag tgaccacggt tgtccggggc gacaggcgtg gtggcgtcga taatcagctt
      661 gtcggtgatc cccgccgggc ttgagcctgg gtcgagttcc agcacggaca tattcggcaa
      721 ctgcaccaaa tcccctgccg gatttacttt cgaggagagc gcccacatca cctgcggcag
      781 gttgaacggg tcaacgtctt catcgaccat aatcaccatc tttacgtagc ccagaccgtg
      841 cggcgtggtc atcgcacgca ggcccaccgc gcgggcaaag ccgccgtagc gttttttggt
      901 ggagataatc gccagcaggc cgtgggtgta catggcgttt accgcctgca cttccgggaa
      961 ctcggctttc agttgctgat acagcggcac acaggtggct ggccccatca ggtagtcgat
     1021 ttcggtccac ggcatgccga ggtacagcga ttcgaaaatc ggcctggtgc ggtaagagac
     1081 tttatcgatg cgcaccacgg tcatgttgcg cccgccggag tagtgcccgg taaactcacc
     1141 gaacggcccc tcgatttcgc gtttacggct ttcgatgacc ccttcgagga tcacttctga
     1201 accccacggc acatcaaaac cggtcagcgg ggcggtggcg atcgggtacg agctttcacg
     1261 cagcgcgcct gccatttcgt actcagattg atcgtatttc agtggcgttg cgcccatcag
     1321 cgtgatgatc ggatcgttac ccagggtgat ggcaatcggc agatcttcac cgcgctcttc
     1381 tgctttatgc agatgcaggg cgatatcgtg catcggcacc ggttgcaggc cgagcttacg
     1441 cttgcccttc acttccatgc ggtagatgcc gacgttctgc ttgccgaagt tatccgggtc
     1501 gagcggatcg cgggaaacca cgcacgcttt gtcgagatag aaaccgccat caccgtcgtt
     1561 taaacgaaac agcggcagga tatcgaacag gttgatctcg tcgccatcga cggtgttctg
     1621 cgcccaggct ggattggcgc ggcgctccgg ggcgatcggg aagttatccc agcggcggat
     1681 aaactcatca atctgctttt tgaccggagt gtttggcggc aggccgaggg aaatcgcgtg
     1741 gttctgccag gaaccgatgg tattcatcgc cacgcgggca tcggtaaaac cgcgaatatt
     1801 atcaaaccac agcgccggtg caccatcgcc gatacgcccg gtggcgttgg cagcggcagc
     1861 cagatccggt tccgcgttca cctcttcgct gattttcagt aactggccgt ggtcatcaag
     1921 cgcctgtaaa aagctgcgta aatcatcaaa tgccattatt cattctcctg agaaaaattc
     1981 cgggcctgcg gcaatccttg ccagcgcctg gcgtggggat gttcgaggcc aaattgatcc
     2041 agcacgcggg ctactacatg gtggacaatg tcatctaccg tttcgggatg gttataaaag
     2101 gcaggcatcg gcggcaccat cgccacgccc atgcgtgaaa gtgcgagcat attttcgaga
     2161 tggatggtgc taagcggcat ttcacgcggc accagcacca gtttgcggcc ttctttgagc
     2221 acgacgtccg ccgcgcgccc taccaggcca tcagcgtaac cagcgcggat accggcgagc
     2281 gttttcatac tgcacggaat aacgatcatg ccgtcggtac gaaaggaacc tgaggagatg
     2341 gtcgccgcct gatccgccgg gttatggctg aagtcagcga gggcagcaac atcgcgggcg
     2401 ctgtaaggcg tttccagttc aatggtggtt ttcgcccact tcgacatcac cagatgagtc
     2461 tcgacattcg gcatctcccg cagcgcttgc agtaatgcca caccaagagg cgcaccggta
     2521 gcccctgtca tcccgacgat cagtttcatt gcttattctc ttaattgttc gtatacgaac
     2581 gttattatta aaatacgcct ctgttctcat cgcggcaagc actgaaaagt aacaacatcc
     2641 ccttcgtcac tgaaaaaccg ctatgatagc ggcagagaat tagcccggag tttacatggc
     2701 gttacgaaat aaagcgttcc atcagttacg acagcttttt cagcagcaca catctcgctg
     2761 gcagcatgag ctacctggcc tgaccaaacc gcagtacgcg gtgatgcgcg ccattgctga
     2821 taagcctggt atcgaacagg ttgcgctgat ggaagcagca gtcagcacca aagcaacgct
     2881 ggcagaaatg ctggcaagaa tggagaatcg tggcttagtc agacgtgatc atgatgccgc
     2941 tgacaagcgg cgtcgctttg tctggctgac tgccgaagga gaaaagatac ttgctgcggc
     3001 gataccgatt ggtgacagcg tggatgagga atttttgggg cgtttgagtg cagaagagca
     3061 ggagctgttt gtgcagctgg tgcgcaagat gatgaacaca taggatgcaa attgccgggt
     3121 aggacgctga cgtgtcttat ccaggcgaca aaaacacaag cttcccaaac aaaagaaaaa
     3181 ggccagcctc gcttgagact ggcctttctg acagatgctt acttactcgc ggaacagcgc
     3241 ttcgatattc agcccctgcg tttgcaggat ttcgcgcaaa cggcgcaggc cttcaacctg
     3301 aatctggcga acacgttcac gggtgaggcc aatttcacga cctacatctt ccagtgttgc
     3361 cgcttcgtac cccagcaaac cgaatcgacg tgccagtact tcacgctgtt tggcgttcag
     3421 ctcgaacagc catttgacga tgctctgctt catatcgtca tcttgcgtgg tatcttccgg
     3481 accgttctct ttttcatcgg ccaggatgtc cagcaacgct ttttcggaat caccacccag
     3541 cggggtgtct accgaggtaa tgcgctcgtt aagacgaagc atacggctga cgtcatcaac
     3601 tggcttatcc agttgctctg cgatctcttc cgcacttggt tcatggtcca gcttatggga
     3661 caactcacgt gcggttcgca ggtaaacgtt cagctccttt acgatgtgaa tcggcaaacg
     3721 aatagtacgg gtttggttca taatcgcccg ttcaatcgtc tggcgaatcc accaggttgc
     3781 gtatgttgag aagcggaaac cacgttccgg gtcaaacttc tctaccgcgc ggatcagccc
     3841 caggttgccc tcttcgataa ggtccagcaa cgccagacca cgattgccat aacggcgggc
     3901 aatttttacc accagacgca agttactctc gatcatccgg cggcgagagg cgacatctcc
     3961 acgcagtgcg cgacgcgcaa aataaacttc ttcttcggcc gttaacagtg gtgaataacc
     4021 aatctcacca aggtaaagct gagtcgcgtc caacacacgc tgtgtggctc cctgcgataa
     4081 cagttcctct tcggccaaat cgttatcact gggttcctct tctactaagg ccttttcgtc
     4141 aaaaacctca actccgttct catcaaattc cgcatcttca tttaaatcat gaactttcag
     4201 cgtattctga ctcataaggt ggctcctacc cgtgatccct tgacggaaca ttcaagcaaa
     4261 agcctggttc cgccgattta tcgctgcggc aaataacgca gcgggtttac ggatttcccc
     4321 ttgtaacgaa tttcaaaatg caagcgtgtt gaactggttc cggtgctacc catggttgct
     4381 attttttgcc ccgccttcac ttcttgttgt tcccggacca gcattgtgtc gttatgggcg
     4441 taggcactca ggtaatcatc attatgtttg atgataatca gattaccgta gccgcgcagc
     4501 gcgttaccgg cataaacaac gcggccatct gcggtcgcga taattgcctg tcctttgctg
     4561 cctgcgatat caatcccctt gttgcccccc tcagaagcgc caaaggtttc gatcactttg
     4621 ccctcagtcg gccagcgcca ggtggagata ggcgtactgg ttgatgtact gctgacagtc
     4681 ggctcggttg tgcttgctgt tggtaccgtt acaggcgctg tgaccgtggt cgcagttggc
     4741 ttgttgttcg gcaacatttt gttagcactc tgttcacccg aagactcaga atacgtaatt
     4801 gtcggttgcg acgcaacagc aacggtggaa ttttgtgcag gcttgatcac aactccttgc
     4861 tctgctgcgt cggcctgggt aatggcattt ccgccagtga ttggcgtacc ggaagcattc
     4921 cccacctgta aggtctgacc aacgttcagc gcgtatggtg cctgaatatt gttgcgctga
     4981 gcaaggtcac ggaaatcgtt gccagtaatc caggcgatat agaaaagtgt gtcgcctttt
     5041 ttcacggtgt atgtactgcc gctataactg cctttcggaa tgttcccata ctgacggtta
     5101 tagacgatgc gtccgttttc catctgtacc ggctgctgag ctactggctg cactggctgg
     5161 atttgcggtt gttgagtagc ctgaatttgt ggctgctgta ccggctgaat ttgcggttgc
     5221 tgcgctgtag acgtcgtccc cattttcggc ggcggcgtaa tcaacatacc agaattggta
     5281 tttgcaggcg cattgccatt gacggaactg accggtgccg gtggatttga agtgtcagaa
     5341 cagcctgcca gccatagcga aaccagtgac aaagccgcaa tgcggcgaac ggtgaatttt
     5401 gggcttcccg cgctcattta tcccccagga aaaattggtt aataaccagt gacataatta
     5461 ccgtgcaagg caccatactg aacactggaa aagatgttca cgatacgctg acctgcggca
     5521 aaataaccag gaaaaatcca ggtatttcct cacgttttaa gccagctcac ccttcactaa
     5581 agggacaaag cgcacggcct ccacggtatc gataataaat tcgcctcccc gacgacgcac
     5641 ccgtttcaaa tactggtgct cctcccctac gggtaagacg agaatcccgc cttcgtccag
     5701 ctgcgtcatt agcgcagttg gaatttccgg cggtgccgcc gtaacaatga tagcgtcaaa
     5761 cggcgcacgt gcctgccaac cttgccatcc atcgccatga cgggttgaaa cattatgtaa
     5821 atcaagattt ttcagacggc gacgcgcctg ccactgcaaa cctttaatcc gttcgaccga
     5881 gcaaacatgc tggacaagat gcgccaggat tgccgtttga tatcccgaac cggtgccaat
     5941 ttccagcacc cgcgactgcg gcgtcaactc gagtaattcg gtcattcgcg ccaccatata
     6001 cggctgcgaa attgtctgcc cctgacctat cggcaaagcg atattgtccc aggctttttg
     6061 ttcaaacgct tcatcaacga atttttcacg cggcacggcg gcaagtgcat tcagcacctg
     6121 ctcatcctga ataccttgcg cacgtaattg atccagaagt gcttgtacgc gtctgcttac
     6181 cattgcgtgc caactcccac gctgtttaac cagtctgaaa ccacatcttg cgcgctatgc
     6241 gcagttaaat ccacatgcag cggcgtgatg gagacatagc cctcatctac cgcagcaaaa
     6301 tcggtccccg gaccggcatc acatttaccg cccggcgggc caatccagta cagcgtattg
     6361 ccgcgcggat cttgctgcgg gatcacctgt tctgccggat gtcgtgtacc gcagcgcgtc
     6421 acgcgaatac ctttgatttg atccaagggt aaatccggaa cgttaatatt aagaatacgc
     6481 ccggtgcgca gcggctcttt acacagtgcg cgcaaaattg aacaggttac cgccgcggca
     6541 gtgtcgtaat gtttatgccc gtcaagcgag acggcaagcg ccggaaaacc taaatgacgg
     6601 ccttccatcg cggcggctac cgtaccggaa taaataacat catcccccag attcggcccg
     6661 gcgttaattc cggacacaac aatgtccggg cgcggacgca tcagagcatt cacgccaaga
     6721 tagacgcaat cggtcggggt tcccatttgc acagcaatat caccattttc aaaggtaaac
     6781 gtgcgcaggg aggattccag tgtcagagaa tttgaagcgc cgctgcggtt acgatcgggg
     6841 gcgaccacct gaacgtcagc aaactcacgc aaggctttcg ccagcgtttg tataccgggt
     6901 gcatgtaccc cgtcatcatt actcagcaat atgcgcataa tcacctgttg tgttgataag
     6961 ttccctgaca acgctggttg caaaactacc cgccggaagc cagaaacgga tctctacggt
     7021 gacgtcatcc caccagttcc agcttaattg ttgcggatac agcagcatcg ctctgcgcgc
     7081 ggcttcaact ttttcgcgca ccagtaaggt ttgtaattca gtttctgcgg cgaccgctgc
     7141 ttgttcgaat gccaacgctt cacgctgagt tccccattcg ccactgcccg gcaatgcggc
     7201 ggttatcatc agctctttat cgttgacgcg acgctgtaat tccaccagtt cttcggtggt
     7261 tgcaacaaac cagctaccac gtccggctaa ttgtagcgca tcgccgtcaa caacttgatt
     7321 aacgtctgct tttttgaggc gctcagcaac aatctgatta aacaacgcac tgcgggctgc
     7381 cgacaaccaa aaactccgtt tattgcgatc gcgcaccgga gtattggttt gcgcccagcg
     7441 cagcgcgccc tgcaagttgc taccgccaat cccaaaacgt tgggcaccga agtagttcgg
     7501 tacacctttt acgcaaatat cgatcagacg ttgttcaacg tcatcgcgat tgctcacttc
     7561 gcgcagaacc agggtaaagg cgttaccttt cagcgcccct aaacgcagct tgcgcttgtg
     7621 ccgcgcatac tccagcacct ggcagccttc caattgaaag gcgctcagat cgggcatttc
     7681 cttgcccggc acgcgagcgc ataaccactg ttctgtaaca gcatgtttgt ctttttgccc
     7741 agcaaagctg acttcacggg catgaatttt caggaatttc gccagtgcat ccgccacaaa
     7801 acgggtattg cagccgtttt tgaggattct aaccagaata tgctcacctt caccatcagg
     7861 ctcaaagccc aaatcttcca ccaccacaaa gtcttccgga ttggctttca gcagcccggt
     7921 gccttgcggt ttaccgtgga ggtaagtgag attatcaaac tcaatcattt tgttgcctta
     7981 atgagtagcg ccaccgcttc acaggcaatc ccttccccac gtccggtaaa tccaagtttt
     8041 tccgtagtag tggctttcac gttaacatca tccatatggc agccgagatc ttcggcaata
     8101 aacacgcgca tttgtggaat gtgcggcaac atcttcggtg cctgagctat gatagtgaca
     8161 tcgacgttgc caagggtata acccttcgcc tgaatacgac gccaggcttc gcgtagcagc
     8221 tcgcggctat cggcaccttt aaatgccgga tcggtatccg ggaacagttt gccgatatcc
     8281 cccagcgccg ccgcgccaag caatgcatcg gtcaacgcat ggagcgccac gtcgccatca
     8341 gaatgcgcca gcaatccttt ttcgtaagga atgcgtacgc caccaatgat aattgggcct
     8401 tcaccgccaa aggcgtgtac gtcaaaaccg tgtccaattc gcattatgta ttctcctgat
     8461 ggatggttcg ggtgaggtaa aactcggcca gtgccaaatc ctccgggcgc gtgactttaa
     8521 tgttatccgc acggccttcg accaactgag gatggaatcc gcaatattcc agcgccgagg
     8581 cttcgtcggt aatagtcgcg ccttcattta gagcgcgcgt cagacagtca tgtaacagct
     8641 cacgagggaa aaattgcggc gtcagcgcgt gccataagcc gttgcgatca acggtatgag
     8701 caatggcatt tttgcccggt tcggcacgtt tcatagtatc gcgcactggt gctgccagga
     8761 tccctcccgt gcggctggtt tcgctcaacg ccaacaatcg cgcgaggtca tcctgatgca
     8821 gacaaggacg agcggcgtca tgcaccaata cccactgcgc gtcgccagcg gctttcaaac
     8881 ctgccagcac ggaatcggca cgctcatcac cgccatctac aacggtgatt tgcggatgat
     8941 tcgccagagg aagttgtgca aaacggctat cgccaggact tatggcaatg acgacacgtt
     9001 tcacccgggg atgcgccagc agcgcatgca ccgagtgttc aagaatggtt tgattaccga
     9061 ttgagagata ttgcttagga cattccgttt gcattcgacg gccaaatccg gccgccggaa
     9121 ccacggcgca aacatccaaa tgagtggttg ccatgttaat tcccgggctg atttatcgat
     9181 tgttttgccc cgcagactgt gcgcgcttcg acgcgtcagg taccagacga taaaaagttt
     9241 cgcccggcct ggtcatgctg agttcattac gcgcacgctc ttcgagcgcc tcctggccgc
     9301 cattgagatc gtcaatttcg gcaaaaagtt gatcgtttcg cgctttaagt ttcgcgtttg
     9361 tagcttgctg tgccgccaca tcatcattga cgcgggtata gtcatgtata ccgttcttac
     9421 cgaaccacag cgaatactgt agccagacca gaatagccag caacagcagc gttagtttac
     9481 ccatcctgcc ccctgaaaaa cggcatcatc atcccatgca tccgaagacg actctacatc
     9541 ctctgttggg gataccgcga caacgcgggc aaatgtacca catttgtcca ttgttacgta
     9601 tacccagggc gtgcagaaca taatctcatt attagttacg gtttgaatta tgaacagagg
     9661 agacaagaaa gtacaaatta gcccagtagc cacataaaca gtgcgccaaa cataatgcct
     9721 actgtcatca gggtgaaaac aatactgtag cgtagctttc cgtccatcaa tgaatgcagc
     9781 gcaatcccca ccactaccgc aacgggcatc agcgccagaa agaaaggcca ggtgtagata
     9841 aagaagaaca gcgtgttaga gccataaatc aacatcggca tcgccagcgc aaataaccaa
     9901 gagataaaac cgaccacggc accaggcagt gaccatgtgg tttcttcatc ctcagtaagg
     9961 ctgtcgttat ttgttagtgt aatgttatgg ctattacgca tatttgatcc tgttactttg
    10021 acgaaccggg catggaaacc cggtggtgtc tcaggatctg ataatatcgt tctgtctcaa
    10081 cagatctaat aattgctgta ccaaatttgt tactaattgt tcaccattga gatgaatttc
    10141 tgccgattca ggcgcttcgt aaacggaatc tattcccgta aagttgcgca gttcaccggc
    10201 acgcgctttc ttatataagc cttttggatc gcgggcttcg caaatcgcca gcggcgtatc
    10261 gacaaacact tcgataaagc gcccttctcc tacgcgttcg cgaaccatct ggcgttcggc
    10321 gcggtgtggc gagataaatg cggtcagcac caccagtccg gcttcaacca tcaaattcgc
    10381 cacttcaccg acgcgacgga tattctcttt acgatcggca tcgctaaaac cgagatcgct
    10441 gcataatccg tggcgaacat tgtcgccatc cagcagatac gtactgacgc cgagtttatg
    10501 taacgcctcc tccagcgccc cggcgaccgt tgatttaccg gacccggaga ggccggtaaa
    10561 ccacagcact acaccacgat gaccgtggtg tagctcgcgt tgttgcacag tgaccggatg
    10621 gctatgccag acgacgtttt cgtcatgcag cgccattatt tatcccccag caaatcgcgc
    10681 gcgccccagt gtggaaagtg gcggcgaacc agggcattca attccagttc gaatgcacta
    10741 aattcagatg gcgcagcagt tgcctggcta actggctcat gcaccatacc ggcacctacg
    10801 gtcacattgc tcaggcgatc gataaaaatc agcccaccgg taaccgggtt ttgctgataa
    10861 cgatctaaca ccagtggctc gtcaaaagtg agatccacca ggccgatgcc gttcagcggc
    10921 aggttttcaa cttcgcgttg ggtaaggtta ttaatatcaa cctgataacg aatgccatca
    10981 acacgagcac gcgtcttctt accggcaatt ttgatgtcgt aactctggcc cggggaaagc
    11041 ggctgttccg ccatccatac cacatccacc gacgcgctct gcacagctgg taacgcttcg
    11101 tctgccgcca gcagcagatc gccacggctg atgtcgatct catccgtcag caccagggtg
    11161 atagcttctc cggcaaaggc ttcttcgcga tcaccatcaa aagtcacgat ccgcgcgacg
    11221 tttgattcca caccagaggg cagcactttt acacgttgcc cgacttccac gcgaccggat
    11281 gccagcgttc cggcgtagcc acgaaaatcg agatttgggc ggttaacgta ctgcaccggg
    11341 aagcgcattg gctgggcatc caccactcgc tgaatctcca cggtttccag cacttcgagc
    11401 agtgtcggac cgctgtacca cggcatactt tcactttgcg aagccacgtt gtcaccttcc
    11461 agtgcggaga gcggcacaaa gcggatatcc agattacccg gcagctgccc ggcaaaggtc
    11521 agataatctt cacgaatacg ggtgaacgtc tcttcactgt aatccaccag atccattttg
    11581 ttgatcgcca cgaccagatg tttgatcccc aacagtgtgg agataaaact gtgacgacgg
    11641 gtttgatcga gcacgccttt acgggcatcg atcagtaaga tcgccagttc acatgtcgat
    11701 gcgccagtcg ccatattgcg ggtgtactgc tcgtgccctg gagtgtcggc gataataaat
    11761 ttacgcttct cggtagagaa atagcggtag gccacgtcaa tggtgatgcc ctgttcacgc
    11821 tcagcttgca ggccgtccac cagcagagcc agatccagct tttcgccctg ggtgccgtga
    11881 cgcttactgt cattatgcag cgatgagagc tgatcttcat agatttggcg ggtatcgtgc
    11941 agcagacgac caatcagggt acttttgccg tcatcgacgc taccacaggt cagaaaacgc
    12001 agcaggcttt tatgttgttg cgcaatcatc caggcttcga cgccgccttc attggcgatt
    12061 tgttgtgcaa gtgcggtgtt catcttaaaa atacccctga cgttttttca gctccataga
    12121 accagcctgg tcgcggtcaa tcacgcggcc ctgacgttca ctggtggtgg aaaccagcat
    12181 ctcttcgatg atttccggca gtgtttgtgc atttgactcc accgcaccgg tcagcggcca
    12241 gcagcccagc gtacggaaac gcaccatccg ttttttgatc acttcgcccg gttgcaggtc
    12301 gatacggttg tcatcaatca tcatcaacat accgtcgcgt tccagaaccg gacgttccgc
    12361 agcgagatac agcggcacaa tgtcgatatt ttccagccag atgtattgcc agatatcctg
    12421 ctcggtccag ttagagagcg ggaagacgcg gatgctttcg cctttgttaa tttgcccgtt
    12481 gtagttgtgc cacagctccg ggcgctgatt tttcggatcc cagcgatgga agcggtcacg
    12541 gaaagagtag attcgctctt tagcacggga tttctcttcg tcacggcgcg caccaccgaa
    12601 ggcggcatca aaaccgtatt tattcagcgc ctgtttcagg ccttcagttt tcataatatc
    12661 ggtatgtttc gcgctgccgt gcacgaatgg attaatcccc atcgccacgc cttccgggtt
    12721 tttatgcacc agcagctcgc agccgtaggc tttcgccgta cgatcgcgga actcatacat
    12781 ctcgcggaat ttccagccgg tatcaacatg cagcaacggg aaaggcagcg tacctggata
    12841 aaacgccttg cgcgccagat gcagcatgac gctggaatct ttaccgatag agtagagcat
    12901 caccggattt gaaaattctg ccgccacctc gcgaataatg tggatgcttt ccgcctccag
    12961 ttgccgcagg tgagtaagtc gtttttgatc cataaccgtt cctttgcaat accgctattt
    13021 tcttgccatc agatgtttcg actataggga gcgtaagaga acgaatgaaa ttaccaatta
    13081 gaatgagtag ttccttaacg gaataacgat ttggcaaagc taatatcaaa aagtgcttaa
    13141 ggcaccggat ttcgggcgtt taggaagatt tgaaattgtt ttagcgcagc ggcagtttca
    13201 tactatggcg gtaaaaaaat ttgcatggta tttaaggact cactatgttt tccgcattgc
    13261 gccaccgtac cgctgccctg gcgctcggcg tatgctttat tctccccgta cacgcctcgt
    13321 cacctaaacc tggcgatttt gctaatactc aggcacgaca tattgctact ttctttccgg
    13381 gacgcatgac cggaactcct gcagaaatgt tatctgccga ttatattcgc caacagtttc
    13441 agcaaatggg ttatcgcagt gatattcgga catttaatag tcggtatatt tataccgccc
    13501 gcgataatcg taagagctgg cataacgtga cgggaagtac ggtgattgcc gctcatgaag
    13561 gcaaagcccc gcagcagatc atcattatgg cgcatctgga tacttacgcc ccgctgagcg
    13621 atgctgacgc cgatgccaat ctcggcgggc tgacgttaca aggaatggat gataacgccg
    13681 caggtttagg tgtcatgctg gaattggcag aacgcctgaa aaatacgcct accgagtatg
    13741 gtattcgatt tgtggcgacc agcggcgaag aggaagggaa attaggcgct gagaatttac
    13801 tcaagcggat gagtgacacc gaaaagaaaa atacgctgct ggtgattaat ctcgataact
    13861 taattgttgg cgataaattg tatttcaaca gcggtgtaaa aacccctgaa gcagtaagga
    13921 aattaacgcg cgacagggcg ctggcaattg cgcgtagtca tggaattgcc gcaacgacca
    13981 atccgggttt gaataaaaat tatccgaaag gcactgggtg ttgtaatgac gcagaaatat
    14041 tcgacaaagc gggcattgct gtactttcgg tggaagcgac taactggaat cttgggaata
    14101 aggatggtta tcagcaacgc gcaaaaacac ctgccttccc ggcgggaaat agctggcatg
    14161 acgtaagact ggataatcaa caacatattg ataaggcact tcctggaaga atagaacgtc
    14221 gctgccgtga tgttatgcgg ataatgctac cgttggtgaa ggagctggcg aaggcgtctt
    14281 gatgggttgg aaaatgggag ctgggtgttc taccgcaggg gcggggaatt ctcagtgata
    14341 tccatcatcg catccagtgc gcccggttta tccccgctgg cgcggggaac acatattttt
    14401 gtcggtcgcc tggaagttca gtttcggttt atccccgctg gcgcggggaa cacggattca
    14461 gaacgccgtc atcctccgca atccacggtt tatccccgct ggcgcgggga acacaattca
    14521 tcatcagatt cacggtcagc gccaggcggt ttatccccgc tggcgcgggg aacacatcca
    14581 gcgcgaccat ttttccctgt aaattttcgg tttatcccca ctggcgcggg gaacacctat
    14641 cttgcgacag ctaaatattc caacaaagcg gtttatcccc gctggcgcgg ggaacacaaa
    14701 gtgctgaagg cgcttaatgc tctcgctggc ggtttatccc cgctggcgcg gggaacactc
    14761 atacagcagg gtgccgtcac cgtcacgcaa cggtttatcc ccgctggcgc ggggaacaca
    14821 ctaagcatac atatctgttt ttaaacaact ttattccaca tcaacaatct accaactaaa
    14881 ttcaaacatt tccttatttt taaagaacac ataacctatt gattatcaac aggaagaaaa
    14941 gaaaccaaac gtaacccatc caaatccacc ggaatacgtc tgttttctcc ccaggtctga
    15001 aattcaaaac ccgactcggt attggtcgcc caggccatca ccacatttcc gcaaccagcc
    15061 agttgggtaa tttgctgcca gatcatctcc cgaatacgtt ttgatgtatc accaacatac
    15121 acaccggcac gcacttccag tagccagatt gcgagccgtc cacgtaagcg cggagggaca
    15181 ttttctgtaa caaccacgac catgctcatc cgccgcgccc ccggtgacca ctatcaccca
    15241 gcgtttcagg ttcagggatg gcaggcggta acatatccgg cgcgggttgt ggtggttcaa
    15301 tttcacctgc agcaaggact tcctcaatta acggtattaa tttgcccgtt aacttagtgc
    15361 tacggaaaat atcgcgacag gctaatctga cttctttatc aggttctgcg ggttgcctcg
    15421 ctgctatttc aaatgccttt ggcacaaccg aatcaaattt aatgatatcg gctatgtcat
    15481 aaacaaatga aagcggtttg ccactatgaa tatatccaat agcgggcgca tatcccgcgg
    15541 ctaatactgc cgcttcagaa ataccgtaca gacatgatgt ggcagcactg atgcagcgat
    15601 tcacaacatc gcctttttcc cagtctttag gatcgtattt gcgaccattc catttcacac
    15661 catattgttt cgccagtaat gcataggtct ggcgaacgcg ggatccctca attccccgta
    15721 gctgatccac tgaacggcga gctggcggtg gctcacgaaa acgtaattca tacattttgc
    15781 gcaccacctt caggcgtaga tcttccgtta aagccagctt tgcctggtag agtaatttat
    15841 ctgcccgcgc ccctccgggt tgtccggaag agtaaacgcg aacgcccgct tcaccgaccc
    15901 agaccagcag tgttcccacc gtggcggcca gatgcaccgc cgcgtgggaa actctcgttc
    15961 ccggttcgag cataatgcag gcgaccgatc ccaccggaat gtgcgtgcgg atcccggttt
    16021 tgtcgatcag cacgaaagcg ccgtccagta cgtcgatttg accgtactgg aggaagatca
    16081 tagaggtgcg atcttttaac gggatcggac tcagtggtac aaacgtcaca cctctgctcc
    16141 gggtttgatc agcatcagac cacagccgaa cgcgcggctt ttgccatacc cctgactgag
    16201 acgttgcaga aataacccgg gatcggtgac cgtaagcatc cccgtataat ccacgctact
    16261 gaactggatc agttgccggg agttttcccg ccgcagttgc tgttgtcggt aggcatcaac
    16321 cgaggtatcc agcagtgtaa aaccgcttct ctccccctgc gctgccagcc agtccagcgc
    16381 cgcctgttgt tgatgcaacc agacatcacg cccttccgcc tgtcccctca cctgccgttt
    16441 cgcctccatc agcagatcgt ggcgcttgcc cgctttacaa attgtgggat tcgcccgcag
    16501 gttgaaacaa agggattgtc cggtacgcag ctccggagca aatgaccggc attcgatagt
    16561 gaaagtttca ctttccgccg ggcgttcctg cgagaggaca aaaaagcgaa acgcaccctg
    16621 aagttcctcc cgacgataaa gaaattgcct ttctttgccg ctagggaaga gatcccacag
    16681 ccactgatgc atcacatatt caccgcgatc caccagatgc agcaactgcg caggcaaaag
    16741 ctgagcggta tgtaaggtta ttcttgagag gtacatggtt cctccttgct gagccacggc
    16801 ccctgattga cggtgcgttc cccaaacagc cactgctggc gatttaaagg aacatcccgg
    16861 cggcgtaata ttttgctcac gaccaggccg tcgtgctccc cttcccacca gcattcatcc
    16921 tgaagtttcg ggagtgagac tttcaggtcg tggaaatgat cctgatactg ttggtatgcg
    16981 ttacgtaaaa catcagacgc gttgccttcg agcagtaacg gcgcaagtgg taatgccagc
    17041 ggatgacttt ttcgccccag ataaagagga aaaactggat ggcgtaaacc gccctgcaac
    17101 tgttcgaggc tgtaaggcgc atcggctgtt gttgccaccg ccaccatcca ccaggcgtcg
    17161 gtgtagtagt cgcgccggga gataatcgcg ctcagaagat cagggtcgct caactcttcg
    17221 cgacggctga aataacgcgc tttacgcacc tcttttggca tctggatcgt gtgataatcc
    17281 cgtgcccagc gcgggttacg gctggcgcaa accaccagtg aatagtggcg attaaacgcg
    17341 tttaagcgtt cggtatcatc acgccgaatc cctaccccgg cggccagcag acccagcaat
    17401 gctgagcgcg aaggcagttc atgggtatga cgcacttcgc cgggggcatc gacgccccag
    17461 gatgccattg gcccatgaag ctgaaaaatc agatattggc tcataagccg ccccttacgc
    17521 gcagataaag tccagcacgt ccttcatgct tccctgtttg ttcatcacgt caaaacttgc
    17581 gcattcggtc ttctgttcgt agaccgtatt catattttcg cgaagcgttg taatacgctg
    17641 caccgccacg tctaactgcc gggtgccatt aatgggttca tagaaggccg cagccagaga
    17701 acgcggttga tcggtgcctt tttccgccag cgcccaggaa gcataggcac ggctggcaaa
    17761 gctattttgt ttaccggttg gggaaacttt aagcgcggct tccgtaaaag cgcgtatggt
    17821 ctgattagct aacgcttcgt caccgccgag gttttctacc agcagatctt tatcaatgca
    17881 gatataggtg tagaaaagtg ctgaaccgaa tccggtttct ccgagatgcc cggcaccggc
    17941 atcttcagag gcctggcgca aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt
    18001 ttcactgaca ccaaacgcat gcgcgacctg gcaggcggct tcaacattaa actcaggttt
    18061 attcgccagc atacgaccaa acatagcgat atctaccgct atacggtctt tacgtaacaa
    18121 cgcgagatct tcctcttttg gcgcgcgctt ttcttctgcc agttgatggg ccagcgcttt
    18181 tacggcgtca aattctgccg ggctgatatg aactaattgt tcagtttcgg cgttagtgag
    18241 cggatctttt ggttttttgt cgtttttagc tttcccaaga taatccgcaa tttttgccgc
    18301 ccattcgatg gcttttttct cttcgattcc tttctcaatc aggatagttg ccgcctcacg
    18361 cgcaattcgc ccactgcgaa taccaatatg gcccgccagt gcctgttcaa aaagtgcaga
    18421 agtgcgccac gcacgtttca gactttgtga ggaaacgcgc agacgcgttg caccacccag
    18481 taccacagtt tttggcgcac cagtatcatc acggttaagg ttggcagcag ggtaagcggt
    18541 taacaaatga agctgaataa atgttgtcat gagatagtcc tttattaatt ttgttcgtta
    18601 tcggcgtcgc cagcctggta atattccagt gcccagcgga tacggataaa ttcggttggc
    18661 cgctgctggc ggcggtgatg attgagcagg tcgtcgctct cctggcacca gcggaaaata
    18721 tcttctgcca aagagataag attgacggaa ccgtttaata atttgaccgc gcgacgtaac
    18781 tgacgcagta aatcatccgg cgtttttact gccgacaggc gagtaaaacg cccctccgac
    18841 attaccgccg ccagctgcgc ggcaaaaggc tgccgttcat caatggcttt tacattggcg
    18901 ctaactgcgg cgaccagcgc cagtgcggta aaacgccact cttgtgcttc aattttccac
    18961 aaagtgtggg tctgcatcag cagagaacga aacccttctg acaagcaaac atcattaacc
    19021 gttgtgctac gccgcagact ggcacgattg ccgcgtttag cctgcaactc ttcgtgccat
    19081 ttacgcagcg tggctttgtg ctcctctttt acaatactca ttcagcagcc tcctgctttt
    19141 tctccctggc ggctttagca ttttgcttct ccgccgatgt tgtaaaatat ttcttgcgtg
    19201 cggtcatgac gcgtttcaaa tcaacgggct cataaggatt ggtgaataca cgctcgtcaa
    19261 aatcctgacg cgcaaataac caaatttcct tttgccattt gccgagtaat tcatctggat
    19321 cctgaccttc ttcaatttgg cgcactaacc tcaggaagcg atgctgagtt ttgttccaga
    19381 agtcgatatc cacaaaactg aaatcacccc gtgcaccttt tggatcggag aaccacgctt
    19441 ctttcaatgc actccgtaac agactcagaa tccgtgaagc cgtttgcgca gccagccgca
    19501 gtttcggtat ctggccttct tttttattga gcagaagcgg gaaatggtgc tcgtaccaac
    19561 agcgcgcttt catgttgtca aaatcataac caaatcccca caggcccact tttgcctgtt
    19621 tcagactgct ggcattaaag agtttcacca ccagcgcggg aagttccgta ttgttttctg
    19681 atttccccgt ttcgataagg cctaaccagt cgcgccagat taaaccgccc ggttgtggtt
    19741 taactgagta aaactcgccg ccctctttaa gtggtacacg gtaaggcgtt aagggatgct
    19801 gccacatggc atagttagcg ccgtaatttt tggtcgtcat caggctcaga agcgcgtcgc
    19861 tctgctcacc gcaaatatcg cagttgccga ctgtggtggt attaaaatca atacgaatac
    19921 gccgtggcat tccccagtac gcctggagtt tattaacctg atcatcggtt accaccgcac
    19981 cggccagttc gctggtacgc gtcgggccaa gccaggggaa aaccagatcg tcaaattttt
    20041 ggggtagcgg taagtcggct tcatcctgcg gcatcacgtt gagccacagt ttgcgccata
    20101 agggtgtttg ttgattgccc tgatattcct gtaattcaat cagagtggtc atcggcccac
    20161 cgccgcgcaa accggtgcga tagcctttgc cacccgctgg tgcattcaac tgtaaagaga
    20221 acaaggccaa tgctaaacaa tgggggcaca gatattccgt tacaccacgc ttgataaaat
    20281 ggtctttatt aaacttcgtc gtttgagcgc cgggaacatc aggcagtagc gaagcgacct
    20341 gaactttatc tcccgtgagc gcctcaaaat cctgcataaa tgaaggggaa tctgggccaa
    20401 actggaaagc atgttctaat gacagcaatg cttcccttag cttttcagct tccagtccgt
    20461 cttcccatat atcatcccaa cgacgatgat cttttggcgc aaagctggtt tgtagtagcc
    20521 ccagcaaaaa ctgccatgcc gcaccttgta aatctgctcg cggcgcggcg atatcgacaa
    20581 cattttcatc agccagatcg actggtgcca gcttgcctgt cgttccgtct ttataacgaa
    20641 cgggcaacca cggggttgtc agaagtgaaa acgagttcat acgcatcctc tcgtaaaaag
    20701 accatccctg gtcggttgtc catccttcca tccttttcgg tgtatcaact attcagagag
    20761 ccctgtcgca ggcatctcaa ccaaccttgc cagtcaatcc ttccatagcc gaatacccgc
    20821 aactttcgtc atcggtgact aaaatcacgt ttgccatctc cggctcttgc cgctgttcaa
    20881 tgcaccactg cctgaacgca tccccttcca gtaaagaaaa ctcatcccga tgttttttcc
    20941 accagcttcg acgcactctg acaacgctca tttcccatgc gtgagcaccg gtagcataag
    21001 gcttcaccac accgtcaata caggtagcca gccacaggga aacagactct tcagccagac
    21061 gtgtcgacag tttttccgga aggtaatcgt tgatactggc ggcatagccg ggcctgaagt
    21121 tcagcacaaa ctttttagcc attgtgcgat cgcagtaata tttgcccact tgctcctgct
    21181 cgctgcgggc aaatccttcc ggcattacca cgtcctcacc gtaaactgat tcaataagaa
    21241 gccgggctgc gtgtggcatc tgaatagcgc cttgcccacg cagcacacgc tgcgtcagcc
    21301 agattcgccc atgatcgggg tagacaaatg cactgttccg catggctctg ccaaaccatt
    21361 catcacccgg agagtcgtcc cagacgggtg ccagaatcag caattcagga ggggaacgct
    21421 cgtcttttcc gtcacgcttt aactgaccat taatatcgcg aatatgccgc tgtaatcgcc
    21481 ccgctcgctg aatcagcaaa tcaacagggg ccaggtcgga gatcatttcg tccaggtcac
    21541 aatcaacgct ctgctctaag acctgagtac aaatgaggac ttttccggca cgctgtgaac
    21601 cgtcttcttt accaaagcgt gccagcgtct ccatttcaat tcgctggcga tcgctaaaag
    21661 caaagcggct atgaaagagt gaaaggctgg aagcgggaat gacgccgcgg gcaagtaact
    21721 gacgatgaac tttaatagcg tcatcgacag aattccggat ccaggcgatg catttcccct
    21781 gacttaccga agattcgata cgcgcaatac attcttgttc gctatggagc caacccacgc
    21841 tcacgctacg ctcaacgtct ttgcgcgtcg ctacccggtg cgaattcaca tccgtttgcg
    21901 tgacatgcgt cagccagggg taatcatctt tttccagcaa tggagcttct tgctggccct
    21961 ctgtgccacg cgcaaaggcg gtaacaagtt tgtcgcgctg ctgttgagat aacgtggcag
    22021 aaagcaaaat aacgctgttt ccaccacgcg cctgtcgctc gatcagccct tcaagaatgc
    22081 aagacatgta agcgtcacag gcgtgaatct catcggccag caggattttg ttactcagcc
    22141 ccagaagccg cagattatta tgtttaaacg gcatcaccgc catcatcgcc tgatccaggg
    22201 tgccgacgcc aatttcagcc agcaacgcct tcttgttact gtcggcaaac caggccgcac
    22261 aaccctggct gaaagcttgt tcatccggtt cttctgaccc gattaaatca ccggaccaga
    22321 gtgattcatt aaagcggtcc attaatgtgc gggcactgtg tgccagcacc agactggggc
    22381 gagattctgg cgaatagaaa gaaagccagg ttttgacgag ccgatcatac atggtattgg
    22441 ccgttgccat tgttggcagg ccaaaaaaca aaccctgagc tttacccgca gccatcaacc
    22501 tgtgcgcaag aataagcgcc gcttctgttt tacctgcacc agtcacatct tccagaataa
    22561 ataactgtgg cccaggctgg ctgatatcca gatccagcac cttttgctgt aacggtgtcg
    22621 gattctcaat aaaaggaaac agcgtattaa ttccggtgaa aggcgcgact tttgctttta
    22681 gaggaaagac ggttaaggcg ttttgagcct gaactaaagt tttctgccag taacctttaa
    22741 tatccattgg gtgtgcgacg cgtggaaaaa atcgcgttga cgaacctgtc cagtctgcga
    22801 gtacgacggt tgcagagata taccaggaaa gttgttttat aagttcaatg ccctcgtcat
    22861 catcccagaa tgcggggatc tctattcgcg gaaacagtgc cttgattgca aggagaaaat
    22921 ctcgtgctgc agctttgtct tcaggcagaa aattatccaa ttcatccata cgatcagggg
    22981 gccgaccatg atgccctgtc gtcacgggca tccacatttc tatcactcgc ctaagtttgc
    23041 gcggagagag cgaagatgaa ggaagtaact cctgacattc acttaaataa tgattccaca
    23101 accagtagcc cagcgttgaa tgagagattt tttcgtaatt ttttctggca ccttcctgaa
    23161 tcttgagttc agggggcagg taaagttgtt gaaaagagtg ggcaaatttt ccaatatcgt
    23221 gccagcacag cgaccaggcg aaaaattgag ccgcctgttc cttgtcagaa atccctaatt
    23281 gataaaaata atcagccagc ccgaagcaat ttcttttaac cattaaatag cccatcgcgg
    23341 ccacatccag cgaatgccag caaagaaggt
//
LOCUS       DAZKFU020000052        23100 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZKFU020000052 DAZKFU020000000
VERSION     DAZKFU020000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.052"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..963
                     /locus_tag="ACHOQQ_RS08115"
                     /old_locus_tag="pgaptmp_001623"
     CDS             <1..963
                     /locus_tag="ACHOQQ_RS08115"
                     /old_locus_tag="pgaptmp_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4469889.1"
                     /translation="CLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLS
                     SYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALL
                     CLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHD
                     VLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVD
                     IIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQ
                     MTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1144..2634
                     /gene="dgcJ"
                     /locus_tag="ACHOQQ_RS08120"
                     /old_locus_tag="pgaptmp_001624"
     CDS             1144..2634
                     /gene="dgcJ"
                     /locus_tag="ACHOQQ_RS08120"
                     /old_locus_tag="pgaptmp_001624"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGU4469890.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2677..3180
                     /gene="yeaK"
                     /locus_tag="ACHOQQ_RS08125"
                     /old_locus_tag="pgaptmp_001625"
     CDS             2677..3180
                     /gene="yeaK"
                     /locus_tag="ACHOQQ_RS08125"
                     /old_locus_tag="pgaptmp_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGU4469891.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3181..3285)
                     /gene="yoaI"
                     /locus_tag="ACHOQQ_RS08130"
                     /old_locus_tag="pgaptmp_001626"
     CDS             complement(3181..3285)
                     /gene="yoaI"
                     /locus_tag="ACHOQQ_RS08130"
                     /old_locus_tag="pgaptmp_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGU4469892.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3455..3901
                     /gene="yeaL"
                     /locus_tag="ACHOQQ_RS08135"
                     /old_locus_tag="pgaptmp_001627"
     CDS             3455..3901
                     /gene="yeaL"
                     /locus_tag="ACHOQQ_RS08135"
                     /old_locus_tag="pgaptmp_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGU4469893.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3858..4679)
                     /gene="nimR"
                     /locus_tag="ACHOQQ_RS08140"
                     /old_locus_tag="pgaptmp_001628"
     CDS             complement(3858..4679)
                     /gene="nimR"
                     /locus_tag="ACHOQQ_RS08140"
                     /old_locus_tag="pgaptmp_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGU4469894.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4776..5957
                     /gene="nimT"
                     /locus_tag="ACHOQQ_RS08145"
                     /old_locus_tag="pgaptmp_001629"
     CDS             4776..5957
                     /gene="nimT"
                     /locus_tag="ACHOQQ_RS08145"
                     /old_locus_tag="pgaptmp_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGU4469895.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMSFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6012..6359
                     /gene="yeaO"
                     /locus_tag="ACHOQQ_RS08150"
                     /old_locus_tag="pgaptmp_001630"
     CDS             6012..6359
                     /gene="yeaO"
                     /locus_tag="ACHOQQ_RS08150"
                     /old_locus_tag="pgaptmp_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGU4469896.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6381..6635)
                     /gene="yoaF"
                     /locus_tag="ACHOQQ_RS08155"
                     /old_locus_tag="pgaptmp_001631"
     CDS             complement(6381..6635)
                     /gene="yoaF"
                     /locus_tag="ACHOQQ_RS08155"
                     /old_locus_tag="pgaptmp_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGU4469897.1"
                     /translation="MKIISFVLPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTTQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6818..7843
                     /gene="dgcP"
                     /locus_tag="ACHOQQ_RS08160"
                     /old_locus_tag="pgaptmp_001632"
     CDS             6818..7843
                     /gene="dgcP"
                     /locus_tag="ACHOQQ_RS08160"
                     /old_locus_tag="pgaptmp_001632"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGU4469898.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            7876..7974
                     /gene="yoaK"
                     /locus_tag="ACHOQQ_RS08165"
                     /old_locus_tag="pgaptmp_001633"
     CDS             7876..7974
                     /gene="yoaK"
                     /locus_tag="ACHOQQ_RS08165"
                     /old_locus_tag="pgaptmp_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGU4469899.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            7977..8051
                     /gene="yoaJ"
                     /locus_tag="ACHOQQ_RS08170"
                     /old_locus_tag="pgaptmp_001634"
     CDS             7977..8051
                     /gene="yoaJ"
                     /locus_tag="ACHOQQ_RS08170"
                     /old_locus_tag="pgaptmp_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGU4469900.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8110..8358)
                     /gene="yeaQ"
                     /locus_tag="ACHOQQ_RS08175"
                     /old_locus_tag="pgaptmp_001635"
     CDS             complement(8110..8358)
                     /gene="yeaQ"
                     /locus_tag="ACHOQQ_RS08175"
                     /old_locus_tag="pgaptmp_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4469901.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8506..8688)
                     /gene="yoaG"
                     /locus_tag="ACHOQQ_RS08180"
                     /old_locus_tag="pgaptmp_001636"
     CDS             complement(8506..8688)
                     /gene="yoaG"
                     /locus_tag="ACHOQQ_RS08180"
                     /old_locus_tag="pgaptmp_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGU4469902.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8692..9051)
                     /gene="yeaR"
                     /locus_tag="ACHOQQ_RS08185"
                     /old_locus_tag="pgaptmp_001637"
     CDS             complement(8692..9051)
                     /gene="yeaR"
                     /locus_tag="ACHOQQ_RS08185"
                     /old_locus_tag="pgaptmp_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGU4469903.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9224..9862)
                     /gene="leuE"
                     /locus_tag="ACHOQQ_RS08190"
                     /old_locus_tag="pgaptmp_001638"
     CDS             complement(9224..9862)
                     /gene="leuE"
                     /locus_tag="ACHOQQ_RS08190"
                     /old_locus_tag="pgaptmp_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGU4469904.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(9989..10912)
                     /gene="dmlR"
                     /locus_tag="ACHOQQ_RS08195"
                     /old_locus_tag="pgaptmp_001639"
     CDS             complement(9989..10912)
                     /gene="dmlR"
                     /locus_tag="ACHOQQ_RS08195"
                     /old_locus_tag="pgaptmp_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HGU4469905.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFATVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQLILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11015..12100
                     /gene="dmlA"
                     /locus_tag="ACHOQQ_RS08200"
                     /old_locus_tag="pgaptmp_001640"
     CDS             11015..12100
                     /gene="dmlA"
                     /locus_tag="ACHOQQ_RS08200"
                     /old_locus_tag="pgaptmp_001640"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGU4469906.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12351..13961
                     /gene="yeaV"
                     /locus_tag="ACHOQQ_RS08205"
                     /old_locus_tag="pgaptmp_001641"
     CDS             12351..13961
                     /gene="yeaV"
                     /locus_tag="ACHOQQ_RS08205"
                     /old_locus_tag="pgaptmp_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HGU4469907.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGALLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            13993..15117
                     /gene="yeaW"
                     /locus_tag="ACHOQQ_RS08210"
                     /old_locus_tag="pgaptmp_001642"
     CDS             13993..15117
                     /gene="yeaW"
                     /locus_tag="ACHOQQ_RS08210"
                     /old_locus_tag="pgaptmp_001642"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGU4469908.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15173..16138
                     /gene="yeaX"
                     /locus_tag="ACHOQQ_RS08215"
                     /old_locus_tag="pgaptmp_001643"
     CDS             15173..16138
                     /gene="yeaX"
                     /locus_tag="ACHOQQ_RS08215"
                     /old_locus_tag="pgaptmp_001643"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGU4469909.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPLDTTHYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKIGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16192..17307)
                     /gene="rnd"
                     /locus_tag="ACHOQQ_RS08220"
                     /old_locus_tag="pgaptmp_001644"
     CDS             complement(16192..17307)
                     /gene="rnd"
                     /locus_tag="ACHOQQ_RS08220"
                     /old_locus_tag="pgaptmp_001644"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGU4469910.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17389..19074)
                     /gene="fadD"
                     /locus_tag="ACHOQQ_RS08225"
                     /old_locus_tag="pgaptmp_001645"
     CDS             complement(17389..19074)
                     /gene="fadD"
                     /locus_tag="ACHOQQ_RS08225"
                     /old_locus_tag="pgaptmp_001645"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGU4469911.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19279..19860)
                     /gene="yeaY"
                     /locus_tag="ACHOQQ_RS08230"
                     /old_locus_tag="pgaptmp_001646"
     CDS             complement(19279..19860)
                     /gene="yeaY"
                     /locus_tag="ACHOQQ_RS08230"
                     /old_locus_tag="pgaptmp_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGU4469912.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(19900..20595)
                     /gene="tsaB"
                     /locus_tag="ACHOQQ_RS08235"
                     /old_locus_tag="pgaptmp_001647"
     CDS             complement(19900..20595)
                     /gene="tsaB"
                     /locus_tag="ACHOQQ_RS08235"
                     /old_locus_tag="pgaptmp_001647"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGU4469913.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLSDIDALAYGRGPGGFTGVRIGIGIAQGLALGAALPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGVWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20653..22563)
                     /gene="yoaA"
                     /locus_tag="ACHOQQ_RS08240"
                     /old_locus_tag="pgaptmp_001648"
     CDS             complement(20653..22563)
                     /gene="yoaA"
                     /locus_tag="ACHOQQ_RS08240"
                     /old_locus_tag="pgaptmp_001648"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGU4469914.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     KLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22695..23039
                     /gene="yoaB"
                     /locus_tag="ACHOQQ_RS08245"
                     /old_locus_tag="pgaptmp_001649"
     CDS             22695..23039
                     /gene="yoaB"
                     /locus_tag="ACHOQQ_RS08245"
                     /old_locus_tag="pgaptmp_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4469915.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5649 a         5920 c         5636 g         5895 t
ORIGIN      
        1 tgtttaacct cgctggcgct cttttgttat ggaaaagaca acatccttga caacaataag
       61 aaaaaaactg gcattctgtt gctggcgctg attccttttt tagtttttcc ccttctggca
      121 cacaatctga gcagttataa cgctgactat tctttgtatg tcgtcgatta ctgtccggac
      181 aaccatactg cgacctgggg aatcaactat acaaaaatat tggtttgtct gtgggcattt
      241 ttactgttct ttattatcat gcgtacacga ttagccagcg aactatggcc gttaatagca
      301 ttattatgtc tggcatcgct atgctgcaac ttacttctac tgactctgga tgagtataat
      361 tataccatct ggtatatcag tcgcgggatt gaagtctcca gtaaactgtt tgttgtgtct
      421 tttctgattt ataacatttt tcaggaactg caactctcca gcaaactggc agttcatgat
      481 gtgctgacca atatttataa tcggcgctac tttttcaaca gcgtagagtc attattgtcg
      541 cgacctgttg ttaaggactt ctgtgtcatg ctggttgata ttaatcagtt caaacgcatc
      601 aatgcccaat ggggacatcg tgtgggtgat aaagtgctgg tttcaattgt cgatattatc
      661 cagcaaagca tccgccccga tgatatttta gcgcgactgg agggtgaggt gtttggcttg
      721 ctatttaccg aactcaatag tgcccaggca aaaatcattg cggaacgtat gcgtaaaaat
      781 gtcgaactcc tgaccggctt tagtaacaga tatgatgttc ctgaacaaat gaccatcagt
      841 attggcacgg ttttttcaac gggtgacacg cgtaatatct cgcttgtcat gacggaagca
      901 gataaagcct tacgcgaagc gaaaagcgag gggggcaaca aagtgattat tcatcatatt
      961 taagtgcaaa aatattcaga gccatgcttt ttgcgtggct tttgcataca atttattact
     1021 attaccccta aatttcccct ccccagcggc gcagagatga gtataattag cgcccctgtg
     1081 ccaggccgca atcgaacttt atctggtttt ctcgtttcac taaccgaagg agtgccattt
     1141 atcatgaaat tgcaccatag aatgctccgg cattttatcg ccgcaagtgt cattgtgctg
     1201 acatcttcct tccttatttt tgaacttgtc gccagcgaca gagcaatgag tgcctatctg
     1261 cgctatatcg tgcagaaagc agactcctcc tttctttatg ataagtatca gaatcagagt
     1321 attgccgcgc atgtgatgcg cgctctcgct gctgagcagt cggaagtgtc gccagaacag
     1381 cggcgcgcca tctgcgaggc ttttgagtct gccaataata cccatggctt aaacctgact
     1441 gcccataaat acccgggctt acgcggcaca ctacaaaccg catccactga ctgcgacaca
     1501 attgtggaag ctgcagcact attacccgct tttgatcagg cagtggaagg caaccgccac
     1561 caggatgatt acggttcagg tcttgggatg gccgaagaga aatttcacta ttatctcgat
     1621 ctcaatgacc gctatgtcta tttttatgag ccggttaatg ttgaatactt tgctatgaat
     1681 aactggtcct tcctgcagtc aggaagtatt ggcatcgatc gcaaagatat tgaaaaggta
     1741 tttaccgggc gtaccgtatt gtcgagcatt taccaggatc agcgtactaa acagaatgtg
     1801 atgagtttgc tgacgccggt gtatgttgca gggcagctaa aagggattgt gctgctggat
     1861 attaacaaaa acaatctgcg gaatatcttt tacactcatg accgccctct cctctggcgt
     1921 tttctcaatg tcacgctaac cgatacagat tcggggcgcg acattatcat caaccagagc
     1981 gaagataatc tgttccagta tgtcagttac gtccatgact taccgggcgg cattcgtgtc
     2041 tcgttatcca ttgatattct ttactttatc acgtcttcgt ggaaaagcgt tctgttctgg
     2101 attttgacgg cgttaatttt gctgaatatg gtgcgaatgc acttccgttt ataccaaaat
     2161 gtgtcgcgag aaaatattag tgatgcgatg actggactgt ataatcgcaa aattttaact
     2221 cctgaactgg agcagcggtt gcagaaactg gtgcaatccg gttcttcggt gatgtttatt
     2281 gctattgaca tggacaagtt aaagcaaata aatgacaccc tcggtcatca ggagggggat
     2341 ttagcgatta cgttattagc tcaggcgatt aaacaatcga ttcgtaaaag tgattatgcc
     2401 atccgactcg gtggcgatga attctgcatc attcttgtcg attcgacgcc gcaaattgca
     2461 gcacaactgc ctgaacgtat cgaaaaacgt ctgcaacata tcgcgccgca gaaagagatc
     2521 ggcttctctt ccggtattta cgcgatgaaa gaaaacgata cgttacatga tgcgtataaa
     2581 gcttccgatg agcgtttata tgtcaataag cagaacaaaa acagccgttc atgataacct
     2641 tctgtggttg tttgcttgta atctcaggag cgtgaaatga ctgaaatggc taaaggaagc
     2701 gtgacgcatc agcggttaat cgctttatta tcacaagaag gtgcagactt ccgcgtcgtt
     2761 acccatgaag cggtaggtaa atgtgaagcg gtgtcagaaa ttcgtggcac cgctctaggc
     2821 cagggtgcta aggcactggt atgtaaagtc aaaggcaatg gcgtaaatca acatgttctg
     2881 gcgatcctcg cagccgatca acaggccgat ctgagccaac tcgccagcca tataggcgga
     2941 ttacgcgctt cgttggccag cccagcggaa gtcgatgaac tcacaggctg tgtcttcggc
     3001 gcgatccccc ctttcagctt ccatccaaaa ctcaaactgg ttgccgaccc tctactcttc
     3061 gaacgatttg atgaaatcgc cttcaatgca gggatgctgg ataaatccgt tattttgaaa
     3121 accgcggatt atttgcgcat tgcgcaacca gaactggtca atttccgccg cactgcgtag
     3181 ctagcctgtc cgttcaataa gcagaacgga caaaaccagc acaaccgcaa tggcaaaaaa
     3241 cgatgacgtg ataatcagtg tctcgacgaa catttgatcg ttcatagcat gcccccggga
     3301 gatagctgtt ttcacgttat tgttagcgtg cacaaatggc agtttgatga cagttcgcct
     3361 gattttttat gcaaaaaacg taaagatttt ctacttcctt cctgcagcaa gcgtaaagta
     3421 agcaggctta ttattttttg gcaaggaaac cacgatgttt gatgtcactt tgctgatcct
     3481 gctcgggtta gctgcgctgg gctttatcag tcataacacc actgtcgccg tttcaattct
     3541 ggtgttgatc attgtccgcg tcacaccgtt aagcaccttt ttcccgtgga ttgaaaaaca
     3601 ggggctaagt atcgggataa tcattctgac gattggcgtt atggccccca tcgccagcgg
     3661 gacgctaccg ccctcaacct tgattcactc gtttctgaac tggaaatcgc tggtggcaat
     3721 tgcagtaggc gttattgtct cctggctggg tgggcgcggc gtgacgttaa tgggcagcca
     3781 gccgcaactg gttgctgggc tgctggtggg cactgtttta ggcgtagcgc tgtttcgcgg
     3841 cgtaccggtt ggcccgctta ttgccgcggg tctggtttcg ctgattgtgg ggaaacagta
     3901 gttaatcccg caatatatcg gcctggcgtt tgccccaggc ctttcttaaa catagtaata
     3961 aaggccgttg ttgaatcata ccccagcgta tgagcaactt tctgtacagt atcgcctttc
     4021 actaatccct gaagcgccat aatcagctgt aactgctgac gccactggcg aaaactcaac
     4081 cccgtctctt ttactatcag tcgggcaaga ttgcgttcac tcatcgcaaa aaagccagcc
     4141 cattgcccca atgaccccca ctcgacgggc cctttcgcca tcatctccac catcgtgcgg
     4201 attttaggat gagaagaaac gggcaggtgc agctgttgtt gtggctgctg agggagttca
     4261 tcaaacagga cctgaattaa tctgcgggtc atcggctcag cacgctgagt cgtcgtcctg
     4321 tttgccaggg ttaaaatcaa ttcccggcat aagggcgata ttttcaatgt gcaacatgtt
     4381 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     4441 acttgattgc tatgttccac cccgcccggt atccaaaccg catattgcgg cggcaccatc
     4501 cacaaagcat tttccaccgt acaggtaatt gcaccatgta gcgccagtat cagctgccct
     4561 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     4621 accgcggctt catggtgtcg gtcgggttca tagccattga gattcaacct atgcatcatt
     4681 ttgtccgaat ttagcgataa tttgtcattt tagcttgatt caacataaca ataaaaacgg
     4741 taaggtacag cctcgtttgt aacaatgaga agcatatgac ctgttcaact tcattaagcg
     4801 gcaaaaacag gattgtcctt atcgctggca ttctaatgat tgccacaaca ttacgcgtca
     4861 cctttaccgg cgcagcaccg ttactggata cgattcgttc cgcttactcg ctgacgacgg
     4921 cgcaaaccgg cttattgacc accctgccat tattagcctt tgcgctaatc tcacctctgg
     4981 ctgccccggt ggcgcgacgt tttggtatgg aacgtagcct gtttgccgcg ttacttttga
     5041 tctgtgctgg tatcgcaatt cgctctctcc cttcgcctta cttattattt ggcggtacag
     5101 cggtcattgg cggtggaatt gcattaggca atgtcttact gccaggatta attaaacgcg
     5161 atttccctca ttccgtcgcc agacttaccg gcgcatattc cctgacaatg ggagctgcag
     5221 cggcactggg atcggctatg gtcgtgccgc tggctttgaa cggttttggc tggcaaggcg
     5281 cgttgctcat gctgatgtct tttcctctgc tggctctttt tttatggctg ccacagtggc
     5341 gaagtcaaca acatgcaaat ttgagtacct cacgcgcctt acatactcgg ggtatctggc
     5401 gttctccgct tgcctggcag gtaacattgt ttcttgggat caactcactg gtctattacg
     5461 tgattattgg ctggcttcca gcgatcctca tcagtcacgg ctatagcgaa gcacaggcgg
     5521 gttcactgca tggtttgctg caactagcca cagcagctcc cggtttgctg atcccacttt
     5581 tcttacatca tgtgaaagat cagcgtggta ttgcagcgtt cgttgccttg atgtgcgcag
     5641 tgggcgcggt cgggctctgc tttatgccag cgcacgcgat cacctggact ctgcttttcg
     5701 gttttggttc cggcgcaaca atgatactgg ggttgacgtt cattggtctt cgggcaagtt
     5761 ctgcgcatca ggcggcggca ctctcgggga tggcacaatc cgtcgggtat ttgctggcag
     5821 cctgtgggcc gccgctgatg ggtaaaatac acgatgctaa cggtgactgg tccgtaccac
     5881 tgatgggggt tgcaatactt tcactactga tggcgatttt cggactttgc gccgggagag
     5941 acaaagaaat tcgctaatat ccggtgctat agtgacgtga ccaatcatgc gtggaaggga
     6001 gaacaaacac gatgaatatt cagtgcaaac gcgtttatga tccggcagaa cagagcgatg
     6061 gttatcgcgt actggtcgac cgcctctggc cgcgtggtat caaaaaaacc gatttagccc
     6121 ttgatgagtg ggataaagaa atcacgccgt caacggaact gcgcaaagcc tttcacggcg
     6181 aagtcgtcga ttttgcaacc tttcgcgagc aatatcttgc agaactggcg caacacgagc
     6241 aagaaggaaa gcggctggcg gacatcgcca aaaaacagcc gctgaccctg ctttactcag
     6301 caaaaaacac cacgcagaac catgcgctgg tgctggccga ctggctacgt agcttgtgat
     6361 tttagtacag catccggcgg ttatttttca ccagccggat ggtcccgtcg ccacaatgcc
     6421 cattcatcaa tcgtttcacc gcccggtaat ttgcaattgt tgctgacccc ttgcgttgtc
     6481 tgcactggaa tgagcgtccc gcccttctgc tggcaataga ccgacgccgg atttgccata
     6541 ccaatctgcg gcggtttagg tgcttctggc tgagaagagg ttgaacaacc agccagaacc
     6601 agcaagcaag gcagaacaaa actgataatt ttcatttatt gatctcacat atttatccag
     6661 gcttagagta tcgcggtatc gttttgtttt gcagcactat ttttattaca ttcactcaaa
     6721 tcatattacg ccttgtttca tctttgttga tgatgtttta tcatgcctgc aaagattaaa
     6781 taatcagcat ttacccgccg tatcctggag ttgtttcgtg tcagatcaga ttatcgcccg
     6841 cgtctcgcaa tcccttgcca aagaacagtc actggaaagc ctggtccgac agcttctgga
     6901 gatgctggaa atggtcactg atatggaatc aacctacctg accaaagtgg atgtcgaagc
     6961 gcgcctgcag catataatgt ttgcccgtaa cagccagaaa atgcacatcc cggagaattt
     7021 taccgtctcg tgggattact cgttatgcaa acgcgccatt gatgaaaact gctttttcag
     7081 cgatgaagtc cccgaccgtt ggggcgactg tattgcggca cgcaatcttg gcatcaccac
     7141 atttctgagc acgccaattc acttaccgga tggatcattc tatggcacgc tttgcgccgc
     7201 cagcagtgag aagcgccagt ggagtgaacg cgcggaacag gttttgcagt tattcgccgg
     7261 actgattgca caatatattc aaaaagaggc gctggtagaa cagctgcgcg aagccaatgc
     7321 cgcgctgata gcgcaatcgt ataccgactc gttaaccggg ttaccaaatc ggcgggcgat
     7381 ttttgaaaat ctgacgacgc tgttttctct cgcccggcat cttaaccata agataatgat
     7441 cgcgtttatc gacctggata acttcaaatt aatcaacgat cgttttggtc ataatagtgg
     7501 cgatctgttt ctcattcagg ttggcgagcg ccttaatacg ctccagcaaa atggcgaagt
     7561 tattggtcgt ctcggcggtg atgagttttt ggttgtttca ctaaacaacg agaatgcgga
     7621 tatttcgtcg ctgcgagaac gcattcagca gcaaatacgc ggagaatatc acttaggtga
     7681 tgttgatttg tattatcctg gtgccagtct tggcatagta gaagtcgatc ctgaaacgac
     7741 cgatgcagac agtgccctac atgctgccga tatcgcgatg tatcaggaga aaaaacacaa
     7801 acagaaaaca ccttttgtcg cgcattcagc gccacattcc tgaggcgtat tcacatcctt
     7861 ttgattggtg ataacatgcg aatcggtatt atttttccgg ttgtaatctt cattacagcg
     7921 gtcgtatttt tagcatggtt ttttattggc ggctatgctg ccccgggagc ataaagatga
     7981 aaaaaacaac gatcattatg atgggtgtgg cgattattgt cgtactcggc actgagctgg
     8041 gatggtggta acgtcacatc taaaaaatag caaaggctgc ctgtgtgcag cctttgtgca
     8101 atttacgcgt taacttttaa tcttcctgta gataaatagc acgacaatcg caccaataac
     8161 ggcaaccacg aagctgccaa aattgaagcc atcgacttta ccaaagccaa acagcgtgct
     8221 gatccaaccg ccgactacgg caccgactat ccccagcagg atagtcataa agaatccacc
     8281 accatcttta cctggcatga tccacttcgc cagaataccg gcaataagcc caaaaataat
     8341 ccatgacaga atgcccattg tttcctcact tatctgtttt gcattagcgg gttagtcgct
     8401 gataaaaagc atagcacaac atcgggaggg caagatttgt gacgagcatc acggaggatt
     8461 tttttgcgat ggcgcagaaa ttgcgccatc aacgatcagt gataattacc aaccacaaac
     8521 atcatgttcg ttttccgtgt cataagaacg cacggtattc accagatctt ttatcacttc
     8581 agccgccact tctggcacca gcaaagtcat cggcgtctct gtttcataat cgacagaaac
     8641 gccattgctg ttattggtga cggtcacggt atacgttgct ttgcccatga ttcatttccc
     8701 gttatgaatg actttccgtt gttgcgcacc ttccatcagg acttcaggag ccacgaagaa
     8761 gtcaatgttg aaataagtat cgtcagtcat ggcttcaatg ttgtgccact tttctggagg
     8821 gaacaccgca aactgccccg cttcgataag gatcacctga tcaggctctg cactgtgttc
     8881 atcagcgtag ccgagatatt tgaccgcccc atgcataacg gaaaggcgtg ggtaaacccc
     8941 cgggcgcgtt cctttatcaa gatgacgttc gaatattccg gcaggcgcag tttgtttatt
     9001 ccagaaaggc gttgagcgcg tatgaatata attctgtggg atttgcagca tccttttccc
     9061 tccttcggtg aatgcgctga aaacggttta ttccagccgt ttcagggtac gcctgataat
     9121 ttgcatttta aataccattt attggttatt ttttagcacc atatcagaga agaatcaggg
     9181 aggattatag atgggaagcc cgcgacaacg cgggctgaaa gcatcaggat tgcagcgtcg
     9241 ccagtcgggc agcgaaaccc acgaacatca aaccaatcag tgagttgccc actttagcca
     9301 gtttcttttt ggtacgtatg tactgcgtga caaaagcacc agatataatc aggaagctca
     9361 aatagcagaa actcaccagt tccagcgtcg tcgccagaat aaagaatgaa attcccgtat
     9421 gtggggcatt aacatcgata aactgtacga aaaacgacac atagaacaaa atggctttcg
     9481 gattagtcag gctcaaaatt aacgcgcgtt taaaaatggc accgtattgg ggctcatcgg
     9541 atttggcctc gttattttta cccttcaggg tcgcgtagag aattttactc cccagataga
     9601 gcaaataaaa tgcaccaaga taacgtacaa tgttgaataa tatcggggtg gtcttaatta
     9661 atgtcgccac tccagcccat gccagaaaca tcaataccgc atcgccaata aatacaccgc
     9721 aggccgcaag ataaccgcct ttcataccgc tactgacgct atttttgagt acaaacaggg
     9781 tatttggccc tggcaccaac acaataaaaa tggccccaac cagataggtc cagtaattca
     9841 gaaccccgta ttcagcgaac acattaacct ctttaattat ctttcgatca tgcgcgatta
     9901 aaggtgaata tgctaaccaa tctgtagcag cttagaaagg agaaaatcag gttttaacct
     9961 gatatcaacc cgataattga atcattaatc aggcatgctc cagtgaaaaa ttcgggtagt
    10021 gctcggcaaa atactggcgt aaaaattcta ccgttatccg cactttcgct gacgtcgcca
    10081 gccttgaaac ataaacggcc cagacgttcg ctggctggta atattccggt agcacttgca
    10141 ctaaatgacc actggcaatg ttttcgctaa catcccacca ggaacgcaga gcaatcccct
    10201 gcccatcgag acaccactga tggacaattt ctccatgatt agacgacagt gggccggtga
    10261 ctttaatcgc atggtggcct tctttattgc gtaattgcca gacaccaaaa ggatgatcgc
    10321 gctctttaat gaccaggcac ggcaatgcag aaagatcggt taaatgtttc ggcgcgccat
    10381 gttgcgcaat aaattccggc gacgcgcaga gaatgcggta attggtcgcc agtttgcggg
    10441 cgattaaatt aggggcgatg tcatcgccaa tgcgaatatc gagatcgaca ccttcattga
    10501 ccaaatcgac cagccggtct tccacatcaa aacgtaattc cagttgcgga tacgccttcg
    10561 ccagcgctga taacgccgga gccaccacct gtcgtccaaa accaaagctg ctgataatac
    10621 gcagcatccc ctgtggcacc tgacgcacgt cagaaagttc gtccatcatc tgaccgacat
    10681 cctgcaaaat cagctgcgcc cattcataaa tccgctctcc ttcttcggta atggtgacgc
    10741 ggcgggtggt gcggtgtaac aacaccacgt ttagcgtttg ctccagcaag gcgatgcgct
    10801 tgctgacgaa cgccggtgaa acgcccagtt cttccgccac ggtggcaaaa ccagcccggc
    10861 gagccaccag cataaagacg cgcaaatcat tcagcagcgg taaattattc atgattcgtg
    10921 ttttatgttt caccagttac ggggattaat tccttttcag tcaattatag gatggtgatg
    10981 ttgtcaattt tgatggtcag gaagtgagaa cccaatgatg aaaacgatgc gtattgctgc
    11041 gatcccggga gacgggattg gcaaagaagt ccttcctgaa gggattcgcg tgttacaggc
    11101 tgccgctgag cgctggggct tcgccttgag ttttgagcaa atggagtggg cgagctgcga
    11161 gtattacagc catcacggta aaatgatgcc ggacgactgg catgagcaac ttagccgttt
    11221 cgatgccatc tattttggtg ccgtcggctg gccggacacc gttccggacc atatttcgtt
    11281 gtggggttcg ctgctgaaat ttcgtcgtga attcgaccag tacgtcaacc tgcgcccggt
    11341 tcgtctcttt cctggcgttc cctgcccgct ggcgggaaaa cagcctggcg acatcgattt
    11401 ttacgtggtc agggaaaaca ccgaaggcga atattcctcg ctcggcggta gagtgaatga
    11461 aggtacagag catgaagtcg tcattcagga atcggtattt acgcgtcgtg gtgtcgatcg
    11521 cattttgcgt tatgccttcg aacttgcgca aagccgccca cgtaagacgc taacttctgc
    11581 tactaaatca aacggtttag ccatcagcat gccgtactgg gatgagcgag tggaagcaat
    11641 ggccgagaat tacccggaga tccgctggga caagcagcat attgatattc tctgcgcgcg
    11701 ttttgtgatg cagccggaac gcttcgatgt ggtggtggcg tccaatttgt ttggcgatat
    11761 cctttccgat cttggcccgg cctgcaccgg caccattggc attgccccat ccgccaacct
    11821 gaatccggaa cgcactttcc cgtcgctctt cgagcctgtc cacggttccg cgccggatat
    11881 ctacgggaaa aatattgcta accctatcgc cacaatttgg gccggtgcaa tgatgctcga
    11941 ttttctcggc aatggcgatg agcgtttcca gcaagcgcat aacggtattc tggcagcgat
    12001 tgaagaagtg attgctcacg ggccgaaaac accggatatg aaaggcagtg ccaccacgcc
    12061 acaggttgcc gacgcgattt gcaaaattat tttgcgttaa ggtcaaacca gtttatttga
    12121 accgcgtcac tgacgcggtt tttttattcg ttctttgcag ttaataacct gcgtcatttc
    12181 accttttatt gtttccgttt cgtgttttat ggctttccgt attcttaatt gtttaattta
    12241 tgtaacatgc aaataatcaa tttgttaaac acaacataca actcattacc ctgcaaataa
    12301 ttacaactgg caaaagccag agacgggctt ttatgccctg gagatgactc atgatgagca
    12361 atgttaagaa aaaagatgtg ccgctgataa gcatcagcct ggtggccatt cttttcatcg
    12421 cagctgcatt aagccttttc ccacaacaat cggccgacgc ggccaacgca atatacactt
    12481 ttgttacgcg tacgttaggt tccgccgtac aggtattggt tttgctggca atgggactgg
    12541 tgatttattt agccaccagt aaatacggca atattcgtct tggcgaagga aaaccggaat
    12601 acagcacgct ctcctggctg tttatgttta tttgtgccgg tttaggttct tctacgcttt
    12661 attggggggt tgctgaatgg gcctattatt atcaaacgcc tggattaaat atcgcaccgc
    12721 gttcacaaca ggcactcgaa tttagcgtcc cttactcctt cttccactgg ggcatcagcg
    12781 cctgggcaac ttatacgctg gcctcattaa tcatggctta tcactttcat gtgcggaaaa
    12841 acaaaggtct gagtctttcc ggcattattg ccgcgattac cggcgttcgc ccgcaaggcc
    12901 catggggaaa actggttgat ttgatgttcc tgatcgccac tgtcggcgca ctgactattt
    12961 cccttgttgt caccgcagca acctttactc gtggactttc cgcactgacc ggtttacccg
    13021 ataacttcac cgtgcaggca tttgtgatcc tgctttccgg cggcattttt tgcctaagct
    13081 catggattgg tatcaacaac ggtttgcaac gtctgagcaa aatggttggc tggggcgcgc
    13141 tcctgctgcc attactggtg ctgattgtcg gtccaaccga atttattacc aacagcatca
    13201 tcaatgccat cggcctgacc acgcaaaact tcctgcaaat gagcttattc accgatccgc
    13261 ttggcgatgg ttcatttacc cgcaactgga ccgttttcta ctggctgtgg tggatctcat
    13321 acacccctgg cgtagcaatg tttgtcaccc gcgtttcccg cggtcgtaag attaaagaag
    13381 ttatctgggg actgatcctc ggcagcaccg tcggttgctg gttcttcttc ggcgtaatgg
    13441 aaagctatgc cattcatcag tttatcaatg gcgtaatcaa cgtcccacag gtgctggaaa
    13501 ctctgggcgg cgaaacagct gtacagcaag ttctgatgtc gttgccagct ggtaaattgt
    13561 tcctcgccgc atacctgggc gtgatgatta ttttccttgc ctcgcatatg gatgcagtgg
    13621 cctacaccat ggcggcgacc agtacgcgta atctccagga aggtgacgat cctgaccgtg
    13681 ggctgcgtct tttctggtgc gtggtgatca ctctgatccc gctttccatc ttgtttaccg
    13741 gtgcttcgct ggaaacgatg aaaaccaccg tcgtgctcac agcccttccc ttcctcgtca
    13801 ttttactggt gaaagtcggc ggatttattc gctggctgaa acaggattac gccgacattc
    13861 cggctcatca agttgaacat tatctcccgc agacaccggt tgaagccctg gaaaaaacac
    13921 cagtgctccc tgcgggaacc gtattcaaag gcgacaactg agcgccatca tcctaacgat
    13981 aaaggtatcc ctatgagcaa tctgagccct gactttgtac tacccgaaaa tttttgcgct
    14041 aacccgcaag aggcgtggac cattcctgcc cgtttttata ccgatcagaa cgcgtttgaa
    14101 cacgaaaaag agaacgtctt cgccaaaagc tggatttgcg tcgctcacag cagcgaactg
    14161 gcgaatgcca atgattatgt gacgcgggag atcattggcg aaagcatcgt gctggtacgc
    14221 ggtcgtgata aggttttgcg cgcgttctat aacgtgtgtc cgcaccgtgg tcatcagttg
    14281 ttgagcggtg aaggaaaagc aaaaaatgtg attacctgcc catatcacgc atgggcattc
    14341 aaactcgatg gcaacctggc ccatgcacgt aactgcgaaa acgtcgccaa tttcgatagc
    14401 gacaaagcgc aactggttcc ggtgcgtctg gaagaatatg ccggattcgt cttcatcaac
    14461 atggacccca acgccaccag cgtagaagat cagttacccg gtctgggcgc gaaagtgctg
    14521 gaagcctgcc cggaagtcca cgatctgaaa ctggcggccc gctttaccac ccgcacgcct
    14581 gccaactgga agaacattgt cgataactat ctcgagtgct atcactgtgg tccggcgcat
    14641 ccaggtttct ccgactccgt acaggttgat cgttactggc acaccatgca cggtaactgg
    14701 acgctgcaat acggtttcgc caaaccgtcc gaacagtcgt ttaaatttga agagggtacg
    14761 gatgcggcat tccacggttt ctggctgtgg ccgtgcacga tgctgaacgt caccccgatc
    14821 aaagggatga tgacggtcat ttatgaattc ccggtggatt ctgaaactac cctgcaaaac
    14881 tacgatattt acttcaccaa tgaagagtta accgacgagc aaaaatcgct gattgagtgg
    14941 tatcgcgatg tgttccgtcc ggaagattta cgtctggttg aaagcgtaca gaaagggctg
    15001 aaatcgcgtg gctatcgtgg tcaggggcgc atcatggccg acagtagcgg tagcggcatt
    15061 tccgaacatg gtatcgccca tttccataat ctgctggcgc aggtgtttaa ggactaatga
    15121 catcggcggc ggtattttcc gccgctgggc tgatttttga tggagtacag caatgtcaga
    15181 ctatcaaatg tttgaagtac aggtgagcca ggttgaaccc cttaccgaac aggtgaaacg
    15241 cttcacgctg gtggcaaccg atggcaaacc attacctgcg tttactggag gaagtcacgt
    15301 cattgtgcag atgagcgatg gtgataacca gtacagcaat gcgtattcac tgctgagttc
    15361 gcctctcgat acaactcatt atcagattgc cgttcgactg gaagaaaact cgcgcggcgg
    15421 ttcccgcttt ttgcatcagc aggtaaaagt gggcgatcgg ttaacgatct caacgcctaa
    15481 taacctgttt gcgctaattc cctcagccag aaagcatctg tttatcgcgg gcggtattgg
    15541 tatcacccct ttcctgtcgc acatggcaga gttgcaacac agcgacgtcg actggcagct
    15601 acattactgc tcgcgaaatc cagaaagttg cgcatttcgt gatgagctag tccagcatcc
    15661 gcaggctgag aaagtccatt tgcatcattc atcaaccgga acacgactgg aattagcgcg
    15721 attattggcg gatatcgaac ctggcacaca cgtttatacc tgtggccccg aggcgctaat
    15781 tgaagcggta agaagtgaag ctgcgcgtct ggacatcgcc gccgatacgc tgcactttga
    15841 gcaatttgct atcgaagaca aaatcggcga tgcatttacc ctggtgcttg cccgttccgg
    15901 aaaagagttt gtggtgccgg aagagatgac tattttgcag gttattgaaa ataataaagc
    15961 cgcgaaagtg gaatgtttat gtcgtgaagg ggtatgcgga acctgcgaaa cggcaatact
    16021 ggaaggtgaa gctgaccatc gggatcagta ttttagcgat gaagagcgtg ccagccagca
    16081 aagtatgttg atctgttgtt cgcgtgcgaa gggtaaacgc ctggtgttgg atttgtagtt
    16141 tgccgaagcc ggatgtggcg ctgagcgcgc ccagtccggc ttcggaagat tttactgcgg
    16201 atattcctgc aataaattgt gtaatgcttc cgccatcagc tcaccacgcc agccggaaat
    16261 cagctccggc aaattgttct gcggtttcag tttccagtgc cagttcagca gttggttgat
    16321 ttgccgacgc gatgccagca attcggcgct gatcttgtgc gtttcgctca cgtcagtaat
    16381 cagcgactta atcgctttaa acgctttacg ataacccggc atgtccatca ggttaagcat
    16441 cggctgcggt aaggcctctt ccggcaatgt ctgcgctttt tccaccagcg ccagcagcgt
    16501 tttaccgtga aagcggattt cgctaccgga taaacccagg ctgtccagtt cgcctaaact
    16561 tcccggcata taacgcgcta ccgaccacaa atgctcttca cgcacgacaa agttcaccgc
    16621 cagatcgcgc tcacgcgcct tgcgcagtcg ccagtcggct aacagttgca gacaggccag
    16681 ttggcgtgtg cgtaattgcc aggcattggt gatatcacgc caggcatctt ccggcgcaac
    16741 gacttcctga cgacgcattt gcatcaggcg gcattcatcc agcgccgcag gtagccagcc
    16801 ggaggcctcc gtttctacca taagcttggc ggtgatcggt aacagatacc agacatccgc
    16861 cgctgcatat tcacactgac gttcggtcag cggtctggcc agccagtcgg tgcgcgattc
    16921 actcttgtcc agcgtaacgc cggaatactc ttccaccatg gaagcgaaac cccatgacat
    16981 cgggcgtccg cagaaggcag caaggatttg cgtgtcaatc aggggttgtg gtaattcgcc
    17041 aaagacattg aggaacactt ccagatcttc actgcctgca tggagaaatt ttgtgatgga
    17101 cggatcgcgc aggatcgctt tcagcggtga ccagtcggtg atcccgagag gatcaattag
    17161 cgccagatgc tcaccatcaa aaagttgaat caaccccagc tgcgggtaat aagtgcgcgt
    17221 acgaacaaat tcagtatcca gggctatcgc cggaaaggca cggacggctt cacacaaaga
    17281 agccagcgca tcgtccgtgg taatcatttg gtaattcaaa ttgtattctc tttagtgggc
    17341 gtcaaaaaaa acgccggatt aaccggcgtc tgacgactga cttaacgctc aggctttatt
    17401 gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc aaaatttttc cgacgttaga
    17461 tttcggtaac tcatcacgaa actccaccag cttcggtact ttgtagcccg tgagctgacg
    17521 gcggcaaaag gtcaccagtg actcttcggt aagcgatgga tcttttttca ctacaaagat
    17581 tttcaccgct tcaccactgg agccggaagg tacgccaaca gccgcgactt cctgtacgcc
    17641 aggatgctgc atgacgacat cttcaatctc gttgggatag acgttaaaac cggaaaccag
    17701 aatcatgtct tttttacgat cgacaatgcg caggaaccct tcttcatcca tcaccgcgat
    17761 gtcgccggtg tgtaaccagc catttttgat gatctcatcg gtagcatccg gacgctgcca
    17821 gtaacccagc atcacctgcg gtcctttgac acaaagctca cccggttgac ccggtggtac
    17881 ttcattatca tcatcatcca ccagtttggc ttccgtcgac ggcaccggca aaccgatgct
    17941 accactatga taatcaatat catatgggtt aacgctgacc agcggcgcac actcggtaag
    18001 gccatagcct tccagcagat actgtcctgt cagtttcacc caacgctctg ccaccacttg
    18061 ctgcactggc atcccaccgc ctgcggaaag atgcagactg gagaaatcca gctgctggaa
    18121 ctctttattg ttcagcaacg cattgaacaa ggtgttaacg ccagtgatag cggtaaacgg
    18181 atatttcgct aactctttta ccaaccctgg aatatcacgc gggttagtga taagcaggtt
    18241 ctgcccaccc agttcgataa acagcaggca gttaatggtc agggcaaaaa tgtgatacag
    18301 cggcagtgcc gttactacca gttctttacc tggatgcagc aacggcccat aggtggcgtt
    18361 gacctgttcc aggttcgcca gcatattgcg gtgagtcagc atcgcgcctt tcgccacacc
    18421 tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc ggcaccagtt cgggtttgac
    18481 gtactgcatc cggtagccgt tatgcagggc gctacgaaat gaaatggcat ctggcagatg
    18541 gtatttcggc accaaacgct tgatgtattt aacaacgaaa ttgactaccg tgccttttgc
    18601 cgtagatagc tgatccccca tacgggtcag aattacgtgc tgaacggcgg ttttatcaac
    18661 cactttttcc agtgtgtgag caaagttaga cacaataaca atcgccgatg cgccgctatc
    18721 gttaagctga tgctcaagct cacgcggggt atacaacggg ttaacgttta cgacgatcat
    18781 cccggcacgc aaaatgccaa acagcgccac cggatattgc aataaattag gcatcatcaa
    18841 cgcaacgcga tcgcctttct tcagccccaa cccttgttgc aaataagcgg caaacgcgcg
    18901 actgcgttct tccagcttgc ggaaggtcat cacctccccc atattcacaa acgcaggctg
    18961 atcggcgtag cgcgcgaccg actgctcaaa catatctacc agagattgat aacggtcagg
    19021 gttgatctcc gtcggaacgt ccgcgggata acggttaagc caaaccttct tcaattcttc
    19081 acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat gatatacatg ccgttaacat
    19141 aatattaact catcatacca gcttgataat tacccaacga aaaggttgcg aagcgcgtca
    19201 ctatttattt ttatctttac cgtaagaatg cagaaacagc ggaccagccg ctgtttcttt
    19261 ttctttaaaa acaagcaatt attcagttac aactgtttgt actctcgcgg ggccgggatt
    19321 ataccagccc catccgccgt agccataggg ccagccacga ccgccataaa accatggatc
    19381 aatcggctga ggcggcataa tcacctgctg ggttaaatgc caacgtttgt aacccgttac
    19441 ttgcatcacc ataaatttat agggcgtatt gccgattttg ccgtcaaccg caccagtgat
    19501 tggcccgact accgtaacca gttgtccacg gaaatccacc gggtccagaa aaccgttcac
    19561 atcggcataa atgcgaccgc gagaaggttc tcccagcgtc ggtctggctc cgctgtccag
    19621 cggtacggta gcaatttcca ggcgggtttt cccttgctgg ttttgtaccg caaccacttt
    19681 gccaccaaag cgtgcctcct gaccaacgta cagctgcggc gcactcatca cccgaactaa
    19741 atcttgttgc ggcgtgggac tactgccttt aatggcgtcc ggcacagtga cacaaccgct
    19801 cagcattagc gcaaacgtac ctgccagtat gcctttgata acattctttt gaaccgccat
    19861 gatgcgactc ctttttttct cagggcatac tcttaagatt cattctttgc ccggaagttt
    19921 cttccatgcg acgttgttac gtaaataaac cggttcggca tgttccaccg ccaccgtttt
    19981 accctcggca aacatctgac aagcaatcgg cagcatatct tctgcagcgg gcagtaaaac
    20041 ttctccgtcg cgcaaaacca gtccgctctc tttaccgaga tccggccagg cttgccagcc
    20101 tgtacctacc gtcacccatt ccccggaaag ctgttgcatc cgttcatgaa cgagttcggg
    20161 tttgagtacg gcttcggttt cttcgccgtg ccagacgccg ttttcatcac gctgatattc
    20221 ggcccagtaa acttcgccca tccgcgcatc aatggcagcc agcacgcgcg ttgcgccgtt
    20281 tttgcgccac gccccctgcg ccatcgtcat gagcgtggag acgccaatca tcggcagcgc
    20341 cgcgccaagt gccagcccct gcgcaatgcc tataccaatg cgcacgccgg taaaaccacc
    20401 ggggccgcgc ccgtaggcca gagcatcaat atcagagagg gaggtaccgc tggtggtcag
    20461 gatatcctgt accatcggta agattcgttg agtatgttca cgagggcaaa gctcaaaatg
    20521 agcgttgaca gtaccgtcgt tccacagggc gacagagcag gcctctgtcg cggtatcgat
    20581 agccagaatt cgcatgggtc ttcgtgctta gatcaataaa aaggcgcgca tcataccata
    20641 ctccgtaaca aattacctgg aggatggtat cgcaaggaaa cgaaccgcac gggcaatgtc
    20701 acgggtgcgc ggcgcgggcg gcagactggc gagaaacgtc gcaccgtaag ggcgcatcac
    20761 aagccgatta tcgcaaatca ccagcacgcc gcgatcatcg gcgtcgcgaa tcagtcgccc
    20821 taccccctgc ttgagagtaa tcaccgcatc cggcagttgt acttcatcga acgggtcgcc
    20881 accgcgcaaa cggcaatctt ccatgcgcgc ttttaacagt ggatcatcag gagaggtaaa
    20941 cggcaatttg tcgataatca ccaatgacag cgtatcgcca cgcacgtcca ccccttccca
    21001 gaaactgctg gttgccacaa gaagcgcgtt accggcgctg acaaactgct gcaacagttg
    21061 ccctttgctg gtttccccct gcaacaaaac gggaagcgtc atggtagcgc ggaactgctc
    21121 ggccagatcg cgcatcatgg cgtgcgaggt acaaagcata aaacaacgac cgttgttagc
    21181 ttcgatgatc ggtcgcagca tagccgccag tttccgcgcg gaacccggtt gattggtttg
    21241 cggcaggttg cgcggcacac agagtaacgc ctggcggctg taatcaaacg ggcttggcaa
    21301 tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg aaatgatgca gatcgtcgtt
    21361 caccgacagc gttgctgagg tgaagatcca gctaccgggc ttttgcgcca ttaactcttt
    21421 gaatttatcc gccacgctga gcggcgtgag agccagagta aaatggcgcg aagtgcattc
    21481 gtaccagtag ctgtagcccg gctgattgat ctcttttagc cgcttcagcc gtgtgcgata
    21541 caacgtggcg cgctcaaatg ccgcatccag caaggcagaa cgccccagtg acagtttcgc
    21601 cacgtcataa caaagttcca gggtgtcatc gagcagtaaa aatgcccgct gaatttgcgg
    21661 attagctaac agctcacgca ggttaccgcg ataacctggt tcaccgagtt gcagacgaaa
    21721 atcctgcgca ctctgagcaa gacgatctgc gcacttttgt aactgctggg tgtcttttaa
    21781 ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg agcagttgtc gactggagag
    21841 tgactgacca aaatactggc tggcaatgtc cggtagctgg tgggcttcgt cgaagatcat
    21901 gacgtccgct tccgggatca actcgccaaa tccactctct ttaaccacca tatccgccag
    21961 aaagagatga tggtttacca ccaccacatc ggcgtccatc gcttttttac gtgctttgac
    22021 cacaaagcaa tctttataca tcgggcagtc gctgccaaga cagttgtcgt tggtgctggt
    22081 gaccagcggc cacgcctgcg aatcttccgc cacgctgacg caggtgctga tatcaccatc
    22141 gactgtttga ttagaccagg agcgcagcag gatcacatcg cttaatattt gtaccggaag
    22201 atcgcccccc gccagcgcct gctgttcgag acgttcgagg cagaggtagt ttgagcgccc
    22261 tttcagcaac gccacgttgc ccgtgtattt caacgccttt gagaccgtcg gcaaatcgcg
    22321 gctatagagc tgatcctgca acgcttttga gccggtcgag ataatgactt tctttttcgc
    22381 ccgcagcgca ggggccaggt aagcgtaggt tttgcccgta ccggttcctg cttccaccac
    22441 cagcggctgg cctttttcta tcgcctgggt gacggctacc gccatctgtc gctgtggttc
    22501 tcgcggctta aagcctggta tcgctttcgc cagctgaccg tctggtgcaa aatcgtccgt
    22561 cacactaccc cctgttgatt tgaacaggga ttatgtcagg atgagggcgc tttcgccagt
    22621 tgaagtggtg acggcgacct cacattgtgg cagtctttgc agcacgaaaa tggaaggata
    22681 atgaggacaa aatgatgact atcgttcgta tcgatgctga agcccgctgg tctgatgtag
    22741 taatccacaa caacacgctc tactacactg gtgtaccgga aaacctcgac gccgatgcct
    22801 ttgagcaaac cgccaacacg ctggcgcaga ttgacgccgt gctggaaaaa cagggcagca
    22861 ataaatcgag tattctggat gccaccattt tcctggccga taaaaacgac ttcgcggcga
    22921 tgaataaagc gtgggatgcc tgggttgtcg cgggtcatgc gccggtgcgc tgcacggtac
    22981 aagcgggttt gatgaacccg aagtataaag ttgaaattaa gattgtggct gcggtgtaag
    23041 ctttatcgaa gcaaaataag tcagacgata atttatcgat aatactggtc ggttttacat
//
LOCUS       DAZKFU020000053        22754 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZKFU020000053 DAZKFU020000000
VERSION     DAZKFU020000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.053"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..1031
                     /locus_tag="ACHOQQ_RS08250"
                     /old_locus_tag="pgaptmp_001650"
     CDS             9..1031
                     /locus_tag="ACHOQQ_RS08250"
                     /old_locus_tag="pgaptmp_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HGU4469916.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSQ
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1031..2011
                     /locus_tag="ACHOQQ_RS08255"
                     /old_locus_tag="pgaptmp_001651"
     CDS             1031..2011
                     /locus_tag="ACHOQQ_RS08255"
                     /old_locus_tag="pgaptmp_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGU4469917.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2008..2766
                     /locus_tag="ACHOQQ_RS08260"
                     /old_locus_tag="pgaptmp_001652"
     CDS             2008..2766
                     /locus_tag="ACHOQQ_RS08260"
                     /old_locus_tag="pgaptmp_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4469918.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            2776..3588
                     /locus_tag="ACHOQQ_RS08265"
                     /old_locus_tag="pgaptmp_001653"
     CDS             2776..3588
                     /locus_tag="ACHOQQ_RS08265"
                     /old_locus_tag="pgaptmp_001653"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4469919.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLISKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            3585..4439
                     /gene="modD"
                     /locus_tag="ACHOQQ_RS08270"
                     /old_locus_tag="pgaptmp_001654"
     CDS             3585..4439
                     /gene="modD"
                     /locus_tag="ACHOQQ_RS08270"
                     /old_locus_tag="pgaptmp_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HGU4469920.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4465..6435
                     /locus_tag="ACHOQQ_RS08275"
                     /old_locus_tag="pgaptmp_001655"
     CDS             4465..6435
                     /locus_tag="ACHOQQ_RS08275"
                     /old_locus_tag="pgaptmp_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4469921.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAVQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINVTTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(6485..6739)
                     /locus_tag="ACHOQQ_RS08280"
                     /old_locus_tag="pgaptmp_001656"
     CDS             complement(6485..6739)
                     /locus_tag="ACHOQQ_RS08280"
                     /old_locus_tag="pgaptmp_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4469922.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6940..7674
                     /locus_tag="ACHOQQ_RS08285"
                     /old_locus_tag="pgaptmp_001657"
     CDS             6940..7674
                     /locus_tag="ACHOQQ_RS08285"
                     /old_locus_tag="pgaptmp_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HGU4469923.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKARHITITAETQGAKVEFTVEQLQQSEYLQLPAFIS
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7676..8287)
                     /gene="emtA"
                     /locus_tag="ACHOQQ_RS08290"
                     /old_locus_tag="pgaptmp_001658"
     CDS             complement(7676..8287)
                     /gene="emtA"
                     /locus_tag="ACHOQQ_RS08290"
                     /old_locus_tag="pgaptmp_001658"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGU4469924.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8387..9301
                     /gene="ldcA"
                     /locus_tag="ACHOQQ_RS08295"
                     /old_locus_tag="pgaptmp_001659"
     CDS             8387..9301
                     /gene="ldcA"
                     /locus_tag="ACHOQQ_RS08295"
                     /old_locus_tag="pgaptmp_001659"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGU4469925.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPICRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAMLNNTHEGTQLTISGHPVLKM"
     gene            9396..11132
                     /locus_tag="ACHOQQ_RS08300"
                     /old_locus_tag="pgaptmp_001660"
     CDS             9396..11132
                     /locus_tag="ACHOQQ_RS08300"
                     /old_locus_tag="pgaptmp_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGU4469926.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            11253..11414
                     /locus_tag="ACHOQQ_RS08305"
                     /old_locus_tag="pgaptmp_001661"
     CDS             11253..11414
                     /locus_tag="ACHOQQ_RS08305"
                     /old_locus_tag="pgaptmp_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469927.1"
                     /translation="MDDKPLQALANKLKTPAQESQRNGFFRLQQVKNNQPRITGIPVN
                     HQGPQPDIA"
     gene            11753..13189
                     /locus_tag="ACHOQQ_RS08310"
                     /old_locus_tag="pgaptmp_001662"
     CDS             11753..13189
                     /locus_tag="ACHOQQ_RS08310"
                     /old_locus_tag="pgaptmp_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469928.1"
                     /translation="MASFKDICPFAPAIPPSSVNTGVRRPPGRLFLSVELKNQILQNM
                     KYLSPQKFSWGDAPWQIVDLSIAGKVNIQVDNNTIITLGTPLNQQHNEFMMVAKWCEW
                     AIQQDGLQENLQKKLHEVLEENRQNKQSEIPQEDLKERLKEIKEDILKENQSASQIED
                     RAEALRRMKECLITRQSMLDLSNLGLTSLPENLPPHLIEFNCSRNVLTALPEVMPKGL
                     RVLECMENFLILLPKVQPPKLMVLKCYENYIIWLPELSTNLRVINCSENFLQFLPPSM
                     PQYLYKLSCAGNNINSIPDEMLENLTRLKVFDCSSNDLISSPRLPPKLIIYYCGENQF
                     KTVRVPQPQSLKVFDCNGNPWDKDNLPTLLKAVEGLKNQQGLEDLLDFLRKEGQIDLE
                     GLEELEDLDDLMDLEFLDDPELLERVKVQEDLELLNQQLGLLSLERQQDSQPVNQQSE
                     HEPEPASRVKRDLSEVEFESTMKRKRFM"
     gene            complement(13358..14428)
                     /gene="dadX"
                     /locus_tag="ACHOQQ_RS08315"
                     /old_locus_tag="pgaptmp_001663"
     CDS             complement(13358..14428)
                     /gene="dadX"
                     /locus_tag="ACHOQQ_RS08315"
                     /old_locus_tag="pgaptmp_001663"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGU4469929.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14438..15736)
                     /gene="dadA"
                     /locus_tag="ACHOQQ_RS08320"
                     /old_locus_tag="pgaptmp_001664"
     CDS             complement(14438..15736)
                     /gene="dadA"
                     /locus_tag="ACHOQQ_RS08320"
                     /old_locus_tag="pgaptmp_001664"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGU4469930.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16066..17598
                     /locus_tag="ACHOQQ_RS08325"
                     /old_locus_tag="pgaptmp_001665"
     CDS             16066..17598
                     /locus_tag="ACHOQQ_RS08325"
                     /old_locus_tag="pgaptmp_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGU4469931.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(17650..18369)
                     /gene="fadR"
                     /locus_tag="ACHOQQ_RS08330"
                     /old_locus_tag="pgaptmp_001666"
     CDS             complement(17650..18369)
                     /gene="fadR"
                     /locus_tag="ACHOQQ_RS08330"
                     /old_locus_tag="pgaptmp_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGU4469932.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18591..20132
                     /gene="nhaB"
                     /locus_tag="ACHOQQ_RS08335"
                     /old_locus_tag="pgaptmp_001667"
     CDS             18591..20132
                     /gene="nhaB"
                     /locus_tag="ACHOQQ_RS08335"
                     /old_locus_tag="pgaptmp_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGU4469933.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20278..20808
                     /gene="dsbB"
                     /locus_tag="ACHOQQ_RS08340"
                     /old_locus_tag="pgaptmp_001668"
     CDS             20278..20808
                     /gene="dsbB"
                     /locus_tag="ACHOQQ_RS08340"
                     /old_locus_tag="pgaptmp_001668"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGU4469934.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(20854..22122)
                     /gene="umuC"
                     /locus_tag="ACHOQQ_RS08345"
                     /old_locus_tag="pgaptmp_001669"
     CDS             complement(20854..22122)
                     /gene="umuC"
                     /locus_tag="ACHOQQ_RS08345"
                     /old_locus_tag="pgaptmp_001669"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGU4469935.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22122..22541)
                     /locus_tag="ACHOQQ_RS08350"
                     /old_locus_tag="pgaptmp_001670"
     CDS             complement(22122..22541)
                     /locus_tag="ACHOQQ_RS08350"
                     /old_locus_tag="pgaptmp_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU4469936.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5412 a         5974 c         5749 g         5619 t
ORIGIN      
        1 tataacttat gtcactaacc agacggaggt ttacacagat tcttgcgtcg acgttgttcc
       61 tgcatcatct gccttctttt gcacaatcag tcaaattctg ggcctcactg acgcttcccg
      121 aagcccaaaa cattacccgc atcgtcagcg caggtgcgcc cgccgatttg ctattgctgg
      181 ctgtcgcgcc agaaaaaatg gtcggttttt cctcttttga ttttgcccgt caggcattaa
      241 ttccattgcc agagcacatt cgccagttac ccaggctggg acgactcgcc gggcgcgcca
      301 gcacactctc gcaggaaggg cttatggcgt tacatcccga tttggtcgtt gattgcggca
      361 atacggatga aacctggatc tcccaggcac ggcaggttag cgaacagaca caaataccct
      421 ggttattgct taacgggaaa ctggagcaat cagcagaaca gttaacaacg cttggcaaaa
      481 cgttaggcga agagcatcgc gccgcagaac aagccaatct cgccagccgc ttcgttggtg
      541 aagctcaggc attcgccacc tcacccgccg ctaacctccg cttttatgct gcgcgcggtc
      601 ctcgagggct ggaaacgggc ttacagggtt cgttgcatac cgaggcggcg gaattattag
      661 gtttgcacaa cgtcgcgcaa atagccgatc gccacggcct gacacaggtt tccatggaaa
      721 atctcctgcg ctggcagccg gatattattc tggttcagga ggccgttact gcagacttta
      781 ttcgtcgtga tccactctgg cagggcgtga aagccgttgc ggaacaacgc atcttatttt
      841 taagtggctt gccctttggc tggctggatg ccccaccggg aatcaaccgt cttctgggat
      901 tacgcagact tcacgcctgg ctggatcccg ccatcaatcg ccagtttaaa agtgacatgc
      961 agcattacgc ccaactgttc tggcattgtt cactcagtga cgccgactat caaaaattgg
     1021 tggcgagcta atgagaatcg taaacggatg tattttgctg ctggcagcta tcagtatcac
     1081 ttttgccgcc gtaagtgggg cttatcatct cgatatgcag caactgctgg cgctaattct
     1141 tcgtcaagaa aatgttcctg tgcaggaaca aattgtcttc tggcaaatcc gcgtgccgcg
     1201 tattcttgct gcgctgttac tgggcgcggc gctggcagga gccggaacca cctatcaggg
     1261 aatgttgcgt aatcccttag tttcaccgga tattttaggc gtctccgccg gtgccggact
     1321 tggtgcctgc acggcaattc tgtggggatt atccattgtt tacatccagc tgtacgcatt
     1381 ttgcggcggg ctgatggtgg tcgcgggcgt ctggttgatt acgcgtcggg tgacccgcca
     1441 cgatccgatc ttaaccctgg tgctggtagg cattgcgtta ggaacgttgt gcggagcggg
     1501 gatatcgcta attaaaacgc tggccgatcc ttacacccag ttaccgtcaa ttaccttctg
     1561 gttgctgggg ggactgtcca ccgtgacctt acgcgatctc tgctatgctg caccgatcat
     1621 tctcataggc tccttgcccc tgttttttct ccgctggcgc atgaatttac tcacgctttc
     1681 cgacgacgaa gcccgttcgc tgggactgaa cgtgacgcgt ctgcgctttg gattgattgt
     1741 ctgcgccaca ctaatcaccg ccagtacggt agcaatagcg ggcataattg gttgggtcgg
     1801 cctggtcgtt ccgcacattg cccgcttgtt gaccggtcat aaccatcagc agctgttgcc
     1861 catggcaatg tgtactggcg cgattctgct cctgcttacc gatacgctgg cacgcagcat
     1921 tggaacaaca gaaatcccac ttggcattct gaccgctttt gttggcgcac cgtttttcct
     1981 gcttttgttg ttacgcggag gtcgccagtg actttgcttg ccgtccgtca tgcgtctctg
     2041 ggttactccc gccatccggt gctacgcgac gtgtccttca ccctgtcgca aggaacaatt
     2101 tgttgtctgt taggagccaa tggttgcggc aaaaccacac tcatgcgctc cattcttggc
     2161 gttataccgt tattaaaagg cgaggtctta ctggatagcc tccctgtgca gacactcagc
     2221 catcgccagc gcgcgcaggc tattgcctgg gtgccgcagg cgcacgacgg tatttttgct
     2281 tttagtgtgc tggatatggt gttgatgggc ctggccccca ccataggcgc tttttccgtt
     2341 cccggtaagc aagaacgcct gaaagccacg gagcaactgg aaaagctggg cattttgcat
     2401 ctggcggcac gacgctggaa tacgcttagc ggcggtgaac gtcagctggc tttaatcgcc
     2461 cgtgcactcg tccagcaacc gcgcttgttg ttactggatg agcctgcctc cagcctcgat
     2521 ttcggtcatc agatccaact actggacacg ctggcacagc tcaaaaataa cggcatgaca
     2581 atgttaatgt cgactcatca tccgctccat gccaacgcca ttgccgacag cattatccag
     2641 gtagagccag atggacgagt aacacaaggc ttaccgaccg aacagttaac aacaaacaaa
     2701 ctggctgccc tgtatcgggt ttctgccgac cagattcatc atcacctctc tgcaattagc
     2761 cactaaggaa caatcatgct cattgatgat attgattttg cagacctcta cctgcagcaa
     2821 ctcaaactgg cccaccgcac agaaaaaacg ccggatcact gggatcaacg ggcggaaaaa
     2881 atggcggaaa attgcgcctc gcctacggac agctacctgc aacaactgat ctcaaaaatt
     2941 gacctacaag gcgctcagac cttattcgat atgggctgcg gaccgggtac cgtatcgctg
     3001 gcgctggctg acaaactcac gacgatttat ggcgttgatt acagtcaggg gatgctgaac
     3061 gttgccgccc gtcgcgccgc cgcgctgaag gcagacaatg tccactggat ccagcgcgcc
     3121 tgggaagagg actggagtga cctgccacgc tgcgatattg ctgtggcttc ccgctccact
     3181 ctggtggctg atatgcgcca ggcaatgagc aaactgaata atcaggcacg gcttcgcgtc
     3241 tatacgactc atctggtcag cacctcgttc gtctctccag ccattcagcg ggcggctggt
     3301 cgggaagtga tcgaattacc caactatatc tttgcgctca acgtcctgta tcagatggga
     3361 atttatgccc acgttgattt tatccgtgga caaaattgcc aacaggataa cagcacctgg
     3421 gaacgttttg agcagaacgt cagctggagt cttggcgcgc ttaacgacga tgagcgtgag
     3481 cggttatatc gctggtatca acaacaagat gctcgtgccc tcgcccctgc cagccgcgac
     3541 tgggcgctca tctggtggga tagcgtaccg caggaggcac tgcgatgatt ttcctttctc
     3601 aggcacaaat cgatgcgtta cttctggaag atatccaggg cggtgacctg accacgagag
     3661 cattaaatat tggacaccag catggctata tagagttttt tctccgtcag ggcggctgcg
     3721 tcagcggcgt ttctgtcgcg tgtaagatgt taactacgct gggattaact attgatgacg
     3781 cggtcagcga cggttcacaa gcgaacgcgg gtcagcggct aatccgtgcg cagggtaatg
     3841 ccgcagcact tcatcaggga tggaaggcaa tccagaatgt gctggagtgg agttgcggtg
     3901 tttctgatta tctcgatcaa atgctggcgt tacttcgtga acgttatcct gatggcaata
     3961 tcgcctgcac ccgaaaagca attccgggca cccgcttact ggcctcgcag gcaattctgg
     4021 ctgccggagg actgattcat cgtgccggat gtgcggaaac catattattg tttgctaacc
     4081 accgccattt tcttcatgac aatcaggact ggtcaggcgc aatcaatcag ttacgtcgcc
     4141 acgcacccga gaagaaaatt gttgttgaag ccgacacgcc gaaagaggca atcgccgcgt
     4201 tacgcgcgca accagacgta cttcagctcg acaaatttag tccgcaacag gcaactgaaa
     4261 ttgcgcaaat agcgccgtcg ctggctcccc actgcacgct ggctctgacc ggcggaatta
     4321 atctgacgac actcaaaaat tacctcgact gcggcattcg cctctttatt acctccgcgc
     4381 cttattacgc ggcaccggct gacatcaaag ttagtctgca acccgcagcc tctatttaat
     4441 tctttgttaa acggaattta ttctatgaga ctcaaaaaac attatctctg cacagcatta
     4501 tcacttgcct ttacccagca ggccgtagcg gttcaggaga gcgactcgct gaccgtatgg
     4561 tccagtccgg tatcatcgac gacgaccacc gttctcgatc aacccaccat gaaggccctg
     4621 gataaacaga atgtcgctca ggcattaagt gtcgtccccg gcgtggtgct gcaaaagtca
     4681 ggcagccgca acgaagaaca ggtaaaagtt cgtggctttg atagtcggca ggtgccggtc
     4741 tatttcgacg gtgtgcccat ttatgttccc tatgacggca acctcgatct ggcgcggatt
     4801 ctgaccaaca atctgggggc agtcgaagtt tccaaagggt attcgtcgct gcttcagggg
     4861 cctaatcaga tgggcggagc cattaatgtc accacccaga agccaacgaa acctctggaa
     4921 gcaagtctgg gatatcgcca gggatggagc cgtagccagg acaatgccta cgatatgcat
     4981 gcttcatttg ccgccagcag cgaactgggg tatttgcaag tcagcggtag tcagctaaag
     5041 caggattttc tcggcctgcc gcatggtgta aataatgata ttgcaggcaa acacggcaag
     5101 atgattaatt catcggctga tgataaacgc ggcattgtga agctcggatt tacaccacgt
     5161 gaaaacgatg aatacacact gacttacatt aagcaggatg gtgaaaaaga taacccgcca
     5221 tacagcggaa atagtggtca aaaatcacgc tactggcagt ggccagagta tgacaaagaa
     5281 agtttttatt atcagggaac gacccaacta aacgatcgtt ttaccctgaa aagtcggctg
     5341 tatcgcgaca cctttgaaaa tacgctgatg atgtacaact cgctggctga tttgaaaaat
     5401 aaaaaaggca gctacagcca ttattccgac tacagcgacg gtgccggatt acaactggca
     5461 gccgatgtgc gtgaaaacga tctgctgtcg tttgccgtta actggaaaga tgacgtacac
     5521 cgggaaaaag gtgcgccgca cgccgcttac gatcgctatg aagatcgtac ctggtcgctc
     5581 gccagtgaat atcaatgggc tgctgccgat aatgtcgatg ttgtggctgg aatcagctat
     5641 gactggcgcg atagtgtaga agcgaaaaaa cacgagaaag atggcagcat cacccattat
     5701 gacgacaaca atcagtcagc ttttaactgg caggtgatgg ggaaatacca ctttgtcaat
     5761 gaagacacgc tggcgctttc gtactatgac cgcacacgct ttccgacgct gaaagaacgc
     5821 tataccacgt ccaaacctgc gtataaccag atagcgattg ttaacccgca gctcaagccg
     5881 gaacgcgctc gcggggtgga tttaacctgg aatggtgcct tcacgcgcga ctggggattt
     5941 gaggtcagcg tttactataa ccgggtgagt gatgccatcc tctcgcacaa tatcgatgcc
     6001 gataccattc aaaatcagaa cagcggcacg gtggattaca gcggtctgga tgccggtatt
     6061 aaggggaaaa tcagcaatat actggatgta ggattgagct acgccctgat ccacgctgac
     6121 gccaaacgca aagacatcgg caagataacc gatctgccaa cgcagacaat gaccgcatgg
     6181 atgactctca aaccgtggga gccgttaagc gtaacgctgt cggaagaggc gcgttcctcc
     6241 agctacagca acagtgacgg ttcacaaaaa gccgccggtt ttgcggtgac ccacattcga
     6301 gccgattaca ccttaggtca tggcttcagc gtcaatgcgt cggtcaataa cctgtttgat
     6361 acccaatatg cctacagtga agggtttatt gaagaaggcc gcaatttctg ggcaggtgtc
     6421 gagtatacgt tctgatgtga aagcggatag cggcatctgt atcgctatcc gccttatgaa
     6481 aatcttattc tcttcgcagg aggcggaata cgcccagaac gagaatagct cccaccaccg
     6541 ccaccaggaa gctgtgcaaa ttaaaaccgc tgatggagcc gccaatgcca aacatggtcg
     6601 ccagccagcc gccgaccacc gcaccgacta tcccgagaat acaggtcagg aaaaatccac
     6661 caccatcacg ccccggcatg attagcttgg cgataatgcc ggctatcagg ccaaaaataa
     6721 tccaggcaat aattcccatg ttcaggccct cgtttcgatg aatcggacgc aagaaatgct
     6781 tacgtcaacg ctctaagtat aggcaagggc gggatcgatg ctgggcgttt gtcggctgat
     6841 ttaagaacaa catgtaaaaa atattcacgc atttgtatta agttttgtta actatgaccg
     6901 ataaaccaaa gacagtttgt cagtcaggag tttttccgcg tgagtcatta ccatgagcag
     6961 ttcctgaaac aaaatccgtt agccgtcctg ggcgtgttac gcgatttgca caaagccgca
     7021 attcctttgc gtatcagctg gaatggcggg cagctgatca gcaaaatact ggtaataacc
     7081 ccggataaac tggtgctgga tttcggcagt caagccgaag acaacatcgc cgtactaaag
     7141 gcacggcaca ttaccattac cgccgaaact cagggtgcga aagtcgagtt tactgttgaa
     7201 caactacaac agagtgaata cttgcagctt ccggcattta ttagcgtacc gcctcccacc
     7261 ttatggtttg tacaacgacg ccgatatttc cgcatctccg ccccactcca tccgccttat
     7321 ttttgccaga ccaaactggc ggataacagt acgttacgtt tccgcctgta tgatttgtcg
     7381 ttaggcggca tgggcgcatt actggaaaca gcaaagcctg ccgggttgca cgaaggtatg
     7441 cgcttcgctc agattgaagt caacatgggg caatggggtg tttttcactt tgacgcccag
     7501 ttaatctcca tcagcgagcg caaagtgatt gatggcaaga atgaaaccat caccactccc
     7561 cgtctgagct tccgttttct taacgtcagc ccgacggtgg agcggcaatt acagcggatt
     7621 attttctctc tcgagcggga agcccgggag aaagcggaca aagtacgcga ctgatttaca
     7681 tcgcgtccag tgcctgctca agtttgtaga tatagcgcgg agcctgcggc gcaggatgat
     7741 ttcgcgctac gtgatcgagg aactcgtcag catccagatc gttgattttg ctgattgcct
     7801 ttttccgatc tgacgagaaa gtccgtagca gcgcacctgc cccgttagcg tatgacacca
     7861 ccagcgcata ttgcagtacc ttcggatctt caatgcctgc cagcgggccg gtttccagaa
     7921 tattcaggta agccgccccc attgaaatat tacgctcagg atttttcagc tcgctggtcg
     7981 tcggctcacc actccagccc atacggcgat atacatcacg tccggaggtt gaagctttta
     8041 actgcatcaa accaatggca ttcgatttac tcaccgcgtt cggattacca cccgattcga
     8101 tagcgataat cgccgtgatc aattgtggat cgacgcccca ggctgcaccg gctttctggc
     8161 ttattggcat ccactgcatc gcacgttgca ccggaacttt cgcgttccac ggcgggttcg
     8221 tgtagtcatg ctttgatgaa caacccgcta ataacacaat caaaaaggca aaccatctca
     8281 atttcacttc ttctatcctt atagcctgga atcgccgttg aggtgacaac ggcacaccta
     8341 agcggcatga tatacatttc gtttctgttg tcagcaagga attgccatgt ctctgtttca
     8401 cttaattgcc ccatcgggtt actgcattaa acagcacgcc gcgctgcgtg gtatccagcg
     8461 ccttaccgac gcggggcatc aagtcaataa cgtcgaggtt attgcccgtc gttgtgagcg
     8521 ttttgcgggt acggaaacgg agcgtctgga agatctcaat tcccttgcca gactgaccac
     8581 ccccaacacc atcgtgctgg ctgtacgcgg cggttacggt gccagtcgtt tactggcaga
     8641 tattgactgg caggcactgg tggctcgcca acaatatgac ccgttgctca tatgcgggca
     8701 tagcgatttt accgccattc agtgcggtct tctggcgcaa ggcaatgtca tcacctttag
     8761 cggccccatg ctggtagcaa actttggcgc ggatgaactg aacaccttta ccgagcacca
     8821 tttctggctg gcgttacgca atgaaacctt taccattgaa tggcaagggg aaggcccgat
     8881 atgtcgggcc gaaggcacgt tgtggggagg caatcttgcg atgctgattt cactgatcgg
     8941 tacaccgtgg atgccaaaaa ttgagaacgg tattctggtg ctggaagata ttaacgagca
     9001 ccctttccgc gtcgaacgta tgctgttgca gctctatcat gctggaattt tgccacgtca
     9061 gaaggcgatt attctcggta gctttagcgg cagtacgcct aatgattatg acgcaggtta
     9121 caacctggag tcagtgtacg cgtttctgcg ttcccgcctg tcgattccac tcattaccgg
     9181 cctcgatttt ggtcatgaac aacgcacggt tacgctgccg ttaggcgcac atgccatgct
     9241 taataacacc catgaaggca ctcagttgac gattagcggt catcctgttc ttaaaatgta
     9301 aaaaaatcac gcttcagggc taagtaaaac gctgtctctg ccgctaatat ggtagggttt
     9361 atataagaga cagcgtaatc aggagtaacc gaccgttgga tgccacaaca ataattagcc
     9421 ttttcatctt aggatccatc cttgtcacca gcagtatatt acttagttca ttttcttccc
     9481 gtcttggcat tcctattctg gttatttttc tggcgatcgg catgctggcg ggagtcgatg
     9541 gcgtcggcgg tatcccgttt gataattacc ccttcgccta catggtaagt aacctggcgc
     9601 tggcgattat tttgctcgac ggcgggatgc gcactcaggc cagttccttt cgtgtggcgt
     9661 taggaccggc gctgtcgctg gcgacgctgg gcgtacttat cacctctggt ttaaccggca
     9721 tgatggcggc gtggctgttt aatcttgatt tgattgaagg cttattaatc ggcgctatcg
     9781 tcggctccac cgatgctgca gcggtctttt ctctgctggg tggtaagggg cttaacgaac
     9841 gtgttggctc gacgctggaa attgaatccg gcagtaatga tccaatggcg gtctttctga
     9901 cgattaccct gattgcgatg atccagcagc atgaaagcag tgtcagctgg atgttcgtgg
     9961 ttgatattct gcagcaattt ggcctcggga ttgtcatcgg tcttggcggt ggttatttac
    10021 tgctgcaaat gattaatcgc atcgccctgc ccgccggttt atatccattg ctggcgttaa
    10081 gcggcggtat tttaattttt gctttaacca cggcgctgga aggtagcggt attctggctg
    10141 tttatctgtg cggttttctg ctgggtaatc ggccgattcg caaccgctac ggcatcctgc
    10201 aaaatttcga cggcctcgcc tggctggcgc aaatcgccat gttcctggtg ctggggctgt
    10261 tggttaaccc aagcgatctg ctgcctattg ccattccggc gctcattttg tccgcatgga
    10321 tgattttctt cgcccgtcct ctttcggtat ttgccggatt gctaccattc cgtggcttca
    10381 atctgcgtga gcgcgtgttt atcagctggg tgggattacg cggcgcggtg ccgattattc
    10441 tggccgtgtt cccgatgatg gcggggctgg agaatgcacg tctgttcttt aatgtcgcct
    10501 tctttgtggt tctggtttca ctgctcttgc agggaacatc actctcgtgg gcggcgaaaa
    10561 aagccaaagt ggtggttccg cccgtcggac gtccggtgtc acgcgttggc ctggatattc
    10621 atccggaaaa tccgtgggag cagtttgttt atcaattgag tgccgataaa tggtgcgtgg
    10681 gcgcggcact gcgcgatttg catatgccaa aagagacgcg tattgcagcc ctgtttcgtg
    10741 ataaccagtt gcttcatcct accggcagca cccgactgcg cgaaggcgat gtattgtgtg
    10801 taattggtcg ggaacgcgat ctcccggcgc tcggtaaact gttcagccag tcgccaccgg
    10861 tcgcgctgga tcaacgcttc tttggtgact tcattctcga agccagcgcc aaatatgctg
    10921 atgttgcgct gatatatggc ctggaagacg ggcgagaata tcgtgataaa cagcaaacgc
    10981 tgggtgaaat tgtccagcag ttgttaggcg cggcaccggt tgtcggtgac caggtagagt
    11041 ttgccggaat gatctggacg gtggccgaga aagaagataa tgaagtgttg aagattggcg
    11101 ttcgagtggc tgaagaggaa gctgaatctt aaatttaatg aagcatctat cgccggttac
    11161 gatgctttgc ttaacgcagc ctgagcctgt atataaactt gtcatttcct gattttgata
    11221 tgcacattcc aacatcagaa gaaggttaaa ttatggatga caaaccattg caggctctgg
    11281 ccaataaact caaaacaccg gctcaagaaa gtcagcgtaa tggcttcttc cgactgcaac
    11341 aggtgaagaa taaccagccc cgcattaccg gtatccccgt caaccatcag gggccccagc
    11401 ctgacatcgc ctgaacatat tcccccacgc aacctgccgc actgacatag cccacacaat
    11461 aggcgtttca tccgcctgta gcaccggatg catgagcgat ttcttatagt tattcctttg
    11521 cttaataacg attatttccg ggtcgttcgc tctcgtcttt tctttaatac gtcagagcta
    11581 cctgggccgt aatacagcat gtaccagacc cgacacggca tttctgacca gggaaaaagt
    11641 caacgatgag tagttatatc agcgcaaatg acgtcctgtg gctcctttgc ggtgaccaca
    11701 ttcacgtctc cggcgctgcc agaggatatt tatttcaagg atacgtagca ttatggctag
    11761 ctttaaagac atctgcccat tcgcccccgc cattccccca tcttccgtta acacaggcgt
    11821 acgacgcccg ccagggcggc tcttcctgtc ggtggaactc aagaatcaaa ttttgcagaa
    11881 catgaagtat ctcagcccgc aaaaattcag ctggggtgat gccccctggc agattgtcga
    11941 tctcagtatc gcgggcaaag tgaacataca ggtggataac aataccatca tcacgttggg
    12001 gactcccctg aatcagcaac ataacgagtt catgatggtc gccaaatggt gcgaatgggc
    12061 tatacagcag gatggtttgc aagaaaacct gcaaaaaaaa ctgcacgagg tcctggaaga
    12121 aaaccggcaa aacaaacagt cagaaattcc ccaagaagac ctgaaagaaa ggctgaaaga
    12181 aatcaaggaa gatatcctga aagaaaacca gtcagcaagc cagattgaag accgggcaga
    12241 agcattgcgc agaatgaagg aatgtctcat aacaagacag agtatgctcg atcttagcaa
    12301 ccttggactg acttcactcc ctgaaaattt gcctccacat ctgattgaat ttaactgcag
    12361 tagaaacgtg ttgaccgcgt taccggaggt aatgccaaag gggctgagag tgcttgaatg
    12421 tatggagaac tttttgatct tgttaccgaa ggtgcagccc ccgaaactga tggtactgaa
    12481 gtgctatgaa aactatatta tctggctgcc tgagctgtcg actaacctga gagtgattaa
    12541 ctgttctgaa aacttcttgc aatttttacc gccgtcgatg ccccagtacc tgtataaact
    12601 gtcctgtgct ggcaacaata ttaactcaat acctgatgag atgctggaga acttgactcg
    12661 cctgaaggta ttcgactgtt ctagtaacga tttgatctct tcaccacggc tgccgcccaa
    12721 actaatcata tactactgtg gagaaaatca gtttaaaact gtacgggtgc cgcagcccca
    12781 gagcctgaag gtgtttgact gtaatggtaa cccttgggac aaagacaatt taccgacgct
    12841 gctcaaagcc gtcgagggcc tgaaaaacca gcagggcctg gaggaccttt tagacttttt
    12901 gcgcaaggaa ggccagattg acctggaggg actggaggag ctggaggacc tggatgacct
    12961 tatggatctg gagttcctgg atgacccgga actcctggag cgcgtgaagg tacaggagga
    13021 cctggaactc ctgaatcagc agttgggcct gttgagtctg gaaagacaac aggactcgca
    13081 gcctgttaat caacaatctg aacatgaacc cgaacctgca tcaagggtga agcgtgattt
    13141 atctgaagtc gagttcgagt caacaatgaa acgtaagcgt tttatgtaga acgcctgtgc
    13201 ggtgagttca ggttccgggg aaatagtgtc catcatttta atgggcacta tcatgggcaa
    13261 tggctctgtc acattaacaa ctacagttgc tgaccagccg gatggatgat tttttttgca
    13321 accagaagac gttgcctccg atccggctta caacaagtta caccgtcaca accgggacac
    13381 gtaacgccag cgcgcacatc aactcatagc ccaccgttcc ggcagcggcg gcgacatcat
    13441 caattttgat ctccttgccc cacagctcaa ccggcgtacc aatacccgcc tgcgggcaag
    13501 gcgttaaatc gaccgccagc atatccatcg aaactgtccc tactgtcatg gtgcgcacgc
    13561 cgtcaactaa aacaggggtg ccggtaggcg catggcgcgg ataaccgtcg gcgtatcctg
    13621 cggcgacaat gccgattcgc tgttcatctc gcgcagtata gcgaccgccg tagcccacac
    13681 gctcgcccgc ttttagcgtc tggacaccaa taatctcact gcttagcgtc atcaccggac
    13741 gtaatccggt attggcgata tcacgccact gaccggacgg cgaagcgcca tacaaaataa
    13801 tgccaggccg aacccagtca aaatgcgctt ccgggtgcca cagagtcgcc gccgaattgg
    13861 acaacgaacg ccgacactcc agcccttccg ccgcctgctc aatacgcgcc atcgcgccgg
    13921 aaattccatc aggatgttcc gcctcggcaa aatgcgacat cagggtcatt tcgccaacat
    13981 tcgccattgc ccgcaactgc tgccagacgg taagcacgcg atcgggctgg aagcccaacc
    14041 gattcatccc actgttcact ttaagataaa tatccaacgg tgcttttagc cgcgcatttt
    14101 gcagtgcttt gagctgccag ttgctgtgta cgcaggtggt caggcggtgc tggtcataaa
    14161 tctccagatc ctgagcatgg aaaaatcctt ccagcatcag gatcggtcct ttccagccgc
    14221 gctcacgtaa cgttattgcc tcttccaggt taagtaatgc aaagccatcg gtagccccga
    14281 gcgcgctcca gatacgctca ataccgtgcc cgtaagcgtt cgcttttacc accgaccaga
    14341 cgcgcgcgtg cggcgcggcc tggcggacaa tggacagatt ctgttttaat gcctgcagat
    14401 cgaggctggc ctgtatcgga cgggtcatct cgtttcctta gctgtgtgcg ccatgtaaat
    14461 ggcccggacg tgatggcgta aatccacggc tgtagcgcgc tacgcttaga tcctcatatg
    14521 ggatcgctgg tgtgcgacca gagagcaggt cgcttaacaa ctgaccggaa ccgcaagcca
    14581 tcgtccagcc gagcgtgccg tgaccggtat tcagccacag atttttaaag cgtgtacgcc
    14641 cgacaaccgg cgtgccgtct ggcgtcatcg ggcgcagacc cgtccagaaa gtcgcctgct
    14701 cgacatgacc gccgcgtgga tagagatcgc gaaccaccat ttctagcgtt tcacgacgcg
    14761 gttgcaacag ctcggtgtta aaaccaacaa tttccgccat cccgccaaca cgaatgcggt
    14821 tatcgaaacg ggtaatggcg attttgtagg tttcatcaag aatggtggat actggcgcac
    14881 catcttcctg cgcaattgga atagtcagcg agtagccttt cagcggatac accggaatat
    14941 caacaatgcc tttgagcatc gccgtcgagt aagaaccaaa cgccatcaca tacgcatcgg
    15001 ccttaatcac ttcatcgcca cacttcacgc cgtagatttg ctcgccgtcg caaagcagtt
    15061 ggtcaacggg cgtattaaag cggaatttaa cccccgcctg ctccgccatc cgcgccagat
    15121 tctgggtaaa tagctgacag tctccggttt catcattggg taactgcagg ccgcccgtca
    15181 gtttgtgcgc cacttctgcc agcgcgggct ccacttccgc caggcggctg gattccagca
    15241 gctgatacgg tacgccggca tcttccagca cggcgatatc gcgggtcgca ttttcatact
    15301 gttgttcggt acggaacagt tgcagcgtcc caccctgacg cccttcatac tgaatattgg
    15361 tttcggcgcg caatgctttc aggcaatcac ggctgtattc cgccagacgc accatccgcc
    15421 ctttgttttc catgtagtgg ctggtgtcgc agttacgtaa catttgccac atccatttca
    15481 actggaactg cgtaccgtcg agacgaactg ccagcggcgc atggcgctgg aacatccatt
    15541 tgatcgcttt taaaggcaca cctggtgccg cccacggtgc agcatatccg ggggagattt
    15601 gcccggcatt cgcggcactg gtctccaggg cggcccccgg ctcccgatca atgacggtga
    15661 cctcatgtcc tgcctgattt aagtaccagg cgctggcaac gcctaccaca ccacttccca
    15721 gtatgacaac tcgcatagcc actccgataa cggtagatac agtaaaagaa taataatcta
    15781 attacatctt gataactcag ttgaaaatat tattcaacat acggcttttt tatggtgagc
    15841 tggctcacat ctccctgact ctgcggcaat atggcgtctt tagtatctcc tgctgaacgc
    15901 cctttttatc cagcataaaa ttccgttcag aagcggatta gtggcactct gataataaga
    15961 aatcgcaaag aaaaaaattc tccggcgacg cggtttttaa caggtgttct atgcttgaaa
    16021 tgaggtgctc cacacagaga gcgccgacaa caatgaggtg cgcgtatggc gacgatcgat
    16081 tctatgaata aggacaccac acgtttgagc gatggacccg actggacgtt cgacctgctg
    16141 gatgtttatc tggcagagat agaccgggtg gcgaaactct accggctgga tacctacccg
    16201 caccagattg aagtgataac ctcagaacag atgatggatg cctactccag cgtcggcatg
    16261 ccaattaact atccgcactg gtcattcggt aaaaagttta tcgagactga acggctgtat
    16321 aagcacggtc agcaaggact ggcctatgaa atcgtcatta actctaaccc gtgtatcgct
    16381 tacctgatgg aagagaacac cattaccatg caagcgctgg tgatggctca tgcctgctat
    16441 gggcataact ctttcttcaa aaacaattac ttattccgta gctggaccga cgccagttcg
    16501 attgtcgatt atctgatttt tgcccgtaaa tatattaccg agtgcgaaga gcgctatggc
    16561 gttgatgaag tagaacggct tctggactcg tgccacgcgc tgatgaacta cggcgtggac
    16621 cggtacaaac gcccgcaaaa aatctcgctg caagaagaga aagcccggca gaaaagtcgc
    16681 gaagagtatc tgcaaagtca ggtcaatatg ctctggcgta ccctgccgaa gcgcgaggaa
    16741 gagaaaacgg ttgctgaagc gcgccgctat ccgtccgagc cacaagaaaa cctgctctat
    16801 tttatggaga aaaatgcgcc gctgctggaa tcatggcagc gtgaaatcct gcgtattgtg
    16861 cgtaaggtga gccagtattt ttatccgcaa aaacagactc aggtgatgaa cgaaggctgg
    16921 gccaccttct ggcactacac catccttaac catctgtatg atgaagggaa agtaacggaa
    16981 cgttttatgc tggagttttt gcacagccac accaatgtgg tcttccagcc gccctataac
    17041 agcccgtggt acagcggcat caacccgtat gccctcgggt tcgccatgtt ccaggatatt
    17101 aaacggattt gtcagtcgcc aacggaagaa gacaaatact ggttcccgga tatcgccggt
    17161 tccgactggc tggaaacgct gcatttcgcg atgcgtgatt tcaaagatga gagttttatc
    17221 agccagttcc tgtcaccgaa agtgatgcgt gatttccgct tcttcaccgt gctggatgat
    17281 gatcggcata attatctgga gatttccgct attcataatg aagagggtta tcgggagatc
    17341 cgtaaccggt tatcgtcgca atataactta agtaatctgg agccgaatat tcagatctgg
    17401 aacgtggatt tgcgcggcga ccgttcgctg acactgcgtt acattccaca taatcgcgca
    17461 ccgctggatc gggggcgcaa agaagtactg aagcatgtgc atcgcctgtg gggatttgat
    17521 gtgatgctgg aacagcaaaa cgaagacggc agcgtcgagt tgctggaacg ttgcccgccg
    17581 agaatgggaa atctgtaaaa acaacaaaaa acccctcgtt tgaggggttt gttttttaaa
    17641 cggaagggat tatcgcccct gaatggctaa atcacccggc agatttttct gcatccggtg
    17701 ccaaatctcg ccactctcat gcccatagcg acgcactgtt tcgtacacct gatcgtgcgc
    17761 gccttcactg cacaacgccg acagtttgtg gtagaagccc agcgccagac tgcgcgcttc
    17821 cggattggcg aaatagtgac gaccaatgcg cgtatacagt cccttcatcc cgttaaggat
    17881 cagaccgtaa atcgggttgc cggaagcaaa cgccaggccg cggaatatgt tgtaatccag
    17941 ctcggcaaag gcatcggcgt gatcggccac ttcattagcg gtagccagca cttcctgcgc
    18001 tttatcggga tgctgacgaa acgcggtgcg aataaaaata gttgaaatat tggtacgcac
    18061 cgacagcaaa ttatcaataa gctgcggcac actttcgtga tccagtcgcg ccagtgtttc
    18121 aaggatattt aaaccagagg tttcccagaa attattcacc ttcgtcggct tgccatgttg
    18181 aatggtcaac cagccatctc gtgccagacg ctgtaacact tcacgtaacg tagtacgcgt
    18241 tacgccaatt aattctgaaa gttcacgttc tgcgggcaaa atagtcccgg gagggaagcg
    18301 gttattccag atactttcaa taatgtactc ttccgcgaaa cccgccgggc tttgcgcctt
    18361 aatgaccata gtgagatttc cataacacag caaaacaaag ttggactcat cataccagag
    18421 ggctaactac gctgatagca gacaaaaaga agaaaaaagg gatcaaggct tcattttccg
    18481 catctctttc cccttgacta tcaagacttg cctcactcct gcctgaccgt taagctttgc
    18541 gtttaaacat aaaaacatca ttgattattt gtaaggtaag ggaaaccatc atggagatct
    18601 cctggggccg cgcgctatgg cgcaattttt tgggccagtc ccccgactgg tacaaactcg
    18661 ccctcatcat tttcttaatc gtaaatccgt taattttcct catcagccct ttcgtcgctg
    18721 gctggttgct ggtagcggaa tttattttca ctctggcgat ggccctgaaa tgctacccgc
    18781 tactccccgg tggtctgttg gctatcgaag cggtattcat cggcatgacc agcgcggaac
    18841 acgtccgtga agaggtggcg gcaaatcttg aagtcttgct gttattgatg tttatggtgg
    18901 cgggtatcta ttttatgaaa cagctgttgc tgttcatatt tacccgtttg ctgttaagca
    18961 ttcgctccaa aatgctgctg tcgctctctt tttgcgtggc ggcagcattc ctctccgcgt
    19021 tcctcgatgc cttaaccgtc gtggcggtgg tgatcagcgt tgcagtcggt ttttatggta
    19081 tttatcatcg cgtagcctct tcccgtaccg aagacaccga cctgcaagac gatagtcata
    19141 tcgacaagca ttacaaagta gttttggaac agttccgtgg ctttctgcgt agcctgatga
    19201 tgcatgccgg tgtcggcacc gcattaggcg gcgtgatgac catggtgggc gaaccacaga
    19261 acctgatcat cgctaaagcg gctggctggc attttggcga tttcttcctg cgcatgtcgc
    19321 cggtgaccgt tccggttctg atttgtggcc tgttaacctg cctgctggta gagaagctgc
    19381 gttggtttgg ctacggtgaa acgctgccgg agaaagtccg cgaagtgctg caacagtttg
    19441 acgatcaaag ccgccaccag cgtacccgtc aggataaaat ccgtctgatt gtccaggcga
    19501 ttattggcgt ctggctggtg actgcgctgg cgttgcatct ggcggaagtt ggcttgattg
    19561 gtttgtcagt cattattctg gcaacatcat tgaccggtgt caccgatgaa catgctatcg
    19621 gtaaagcctt caccgaatct ctgccattca ccgcactgtt gacggtgttt ttctcggtag
    19681 tcgcggtgat tatcgaccaa caactgtttt cgccgattat tcagtttgtg ttgcaggcat
    19741 cggaacatgc tcagctgtcg ttgttctata ttttcaacgg actgctgtca tccatttcgg
    19801 ataacgtctt cgtggggacg atttatatca acgaagcgaa agcggcaatg gaaagtggcg
    19861 ctatcacgtt gaagcaatac gagctgctgg cggtcgccat taataccggt accaatctgc
    19921 cctctgtcgc tacgccgaac ggtcaggctg cgttcctgtt cctgctgacc tctgcactcg
    19981 cgccattgat tcgcctctct tatggccgca tggtgtggat ggccctgcct tacaccctcg
    20041 tcctgacact cgtcggactg ctctgcgtcg agtttacgct tgcccctgta accgaatggt
    20101 ttatgcaaat gggctggata gcaacgcttt gataacaact taccgggcat ataaatgccc
    20161 ggtttgcctt ttcgcccgat aattgtccaa ttgcgtccta tttttgctgc ctcctggtgg
    20221 cgcagcgaat gaattggttt aaactgcgca ctctatgcat attgcaggga aatgattatg
    20281 ttgcgatttt tgaaccaatg ttcacaaggc cggggcgcgt ggctgttgat ggcgtttact
    20341 gctctggcac tggaactgac ggcgctgtgg ttccagcatg tgatgttact gaaaccttgc
    20401 gtgctctgta tttatgaacg ctgcgcatta ttcggcgttc tgggtgctgc gctgattggc
    20461 gcgatcgccc caaaaacgcc actgcgttat gtagcgatgg ttatctggtt gtatagtgcg
    20521 ttccgcggtg tgcagttaac ttacgagcac accatgcttc agctctatcc ttcgccgttt
    20581 gccacctgtg attttatggt tcgtttcccg gaatggctgc cgctggataa atgggtgccg
    20641 caagtgtttg tcgcctctgg cgattgcgcc gagcgtcagt gggatttttt aggtctggaa
    20701 atgccgcagt ggctgctcgg tatttttatc gcttacctga ttgtcgcagt gctggtggtg
    20761 atttcccagc cgtttaaagc gaaaaaacgt gatctgttcg gtcgctaatc cattcggcgc
    20821 tcctgcggga gcgctttttt cctgccgcta tatttatttg acccgcagta aatcagaact
    20881 tcgcgttgta taacgtggtg aaagcatggc tcgcttcatc tgccattgtt gctggatccc
    20941 ctgcccggca aaatagagtg ttcctctgcc ctctttagca ttcagtgtat ccattaccgt
    21001 catcaattgc tcactcccgg ggcgcggtgc gttgtcatcg aataaattga gctgcgcgac
    21061 tccctgactg aagaaatccc ccagcatcac gcccgctttt tggtagcgat ggcccgcttg
    21121 ccagatggca tccagagatc gcgtagcggc gttaatgata tccctgctgt cctgagtggg
    21181 cgtcagcagt tttaccgacg cgctattgcc gtaataaggt tcattgagcg caaatggtga
    21241 cgtcttaata aatgtggaga taaaccgaca atattgatgc tcactgcgaa gtttttccgc
    21301 cgcccgggca gcgtaactac aaatggcctg ccgcatcgac gtataatccg tgatgcgttc
    21361 accaaacgag cgggaacaga taatttcctg ctttgtcggt gcaaattctt ccagttgcaa
    21421 acagggttca ccgcgcagtt cacgcaccgt tctttcgagc acgacattaa aatgtttacg
    21481 gataaaccgg atatctgtat ccgccaaatc gagaacggtt ttgatcccca tcgcgtccag
    21541 ttttttgctg atccgccgtc caatccccca gacgtcatcc acggggagag cagacattaa
    21601 tttacgctgg cgttccagat ttgataaatc caccacccca cccgtctgcc gctgccattt
    21661 ttttgccgca tgattggcaa gcttagccag cgttttggtc tgggcgatcc ccacaccaac
    21721 agtaagatgg gtacgttgta gcaccgttgc gcgaatttct ctgccaaagt cagtcagatc
    21781 gcgacaatta cgcacacctg tcagatcgca gaatgcctca tcaatactgt aaatctcgac
    21841 gcggggcgat agttcttcca gcgtcgacat cacccgattg ctcatgtctg cgtaaagctc
    21901 ataattgctg ctaaagcaaa ccacgccaca gcgacgaaac agatcttttt gtttgaacca
    21961 gggatcgccc attttaacgc caagcgcctt tgcctcagcg tttcgggcga taacgcaacc
    22021 gtcattattc gatagcacaa ccaccggttt accccataaa tcagggcgaa acaccgtctc
    22081 acagctggca taaaacgcgt ttacatcaca gagggcaaac atcagcgcat cgccttaacg
    22141 acgtggatca ccacaccaaa gacatccagc gtatcttcac tactgatggt aatgggcgag
    22201 tacgcgctgt tcatgggaat aagctgtacc gtcgggcgta gttgcaattt tttcaccgta
    22261 aactcgccgt caacagcagc gatgacaata tcaccatggc tggcggtaat agcgctatcg
    22321 acaatcagta aatcaccgtc actaattcca ccatcaatca tagaatcacc acttgctttg
    22381 acgaagtaag tcgcgctggg atgctggatc aacagttgat tcagatcgat gcgctgttca
    22441 acgtaatctg cggccggtga aggaaagcca cactgaacaa gatcgctaaa tagcggaaaa
    22501 gtcacaattt cgcggagatc cgcaggcttg ataaacaaca taataatctg cctgaagttg
    22561 tactgttttt atatacagta gtctgttctt gccagcagat caatactgat tcaggctatc
    22621 aatatttgtc gctgcataga ctgctgattt ttcgttctct tatcctgtgc tcacgcggcc
    22681 ttctggcgac gacgctcatc cagcagaaat gaaaaatatc acccggctaa aaaatagaat
    22741 agaagcattc gcca
//
LOCUS       DAZKFU020000054        22434 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZKFU020000054 DAZKFU020000000
VERSION     DAZKFU020000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.054"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(181..256)
                     /locus_tag="ACHOQQ_RS08355"
                     /old_locus_tag="pgaptmp_001671"
     tRNA            complement(181..256)
                     /locus_tag="ACHOQQ_RS08355"
                     /old_locus_tag="pgaptmp_001671"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Phe,seq:gaa)
     gene            complement(362..1069)
                     /gene="yqgA"
                     /locus_tag="ACHOQQ_RS08360"
                     /old_locus_tag="pgaptmp_001672"
     CDS             complement(362..1069)
                     /gene="yqgA"
                     /locus_tag="ACHOQQ_RS08360"
                     /old_locus_tag="pgaptmp_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGU4469937.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHEPF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1222..1305
                     /locus_tag="ACHOQQ_RS08365"
                     /old_locus_tag="pgaptmp_001673"
     CDS             1222..1305
                     /locus_tag="ACHOQQ_RS08365"
                     /old_locus_tag="pgaptmp_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4469938.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            1468..3603
                     /gene="speC"
                     /locus_tag="ACHOQQ_RS08370"
                     /old_locus_tag="pgaptmp_001674"
     CDS             1468..3603
                     /gene="speC"
                     /locus_tag="ACHOQQ_RS08370"
                     /old_locus_tag="pgaptmp_001674"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU4469939.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQHWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESQEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAVEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3653..4909)
                     /gene="nupG"
                     /locus_tag="ACHOQQ_RS08375"
                     /old_locus_tag="pgaptmp_001675"
     CDS             complement(3653..4909)
                     /gene="nupG"
                     /locus_tag="ACHOQQ_RS08375"
                     /old_locus_tag="pgaptmp_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGU4469940.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5111..6190)
                     /gene="mltC"
                     /locus_tag="ACHOQQ_RS08380"
                     /old_locus_tag="pgaptmp_001676"
     CDS             complement(5111..6190)
                     /gene="mltC"
                     /locus_tag="ACHOQQ_RS08380"
                     /old_locus_tag="pgaptmp_001676"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGU4469941.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6255..6530)
                     /gene="yggX"
                     /locus_tag="ACHOQQ_RS08385"
                     /old_locus_tag="pgaptmp_001677"
     CDS             complement(6255..6530)
                     /gene="yggX"
                     /locus_tag="ACHOQQ_RS08385"
                     /old_locus_tag="pgaptmp_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGU4469942.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6558..7610)
                     /gene="mutY"
                     /locus_tag="ACHOQQ_RS08390"
                     /old_locus_tag="pgaptmp_001678"
     CDS             complement(6558..7610)
                     /gene="mutY"
                     /locus_tag="ACHOQQ_RS08390"
                     /old_locus_tag="pgaptmp_001678"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGU4469943.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7771..8490
                     /gene="trmB"
                     /locus_tag="ACHOQQ_RS08395"
                     /old_locus_tag="pgaptmp_001679"
     CDS             7771..8490
                     /gene="trmB"
                     /locus_tag="ACHOQQ_RS08395"
                     /old_locus_tag="pgaptmp_001679"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGU4469944.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8490..8816
                     /gene="yggL"
                     /locus_tag="ACHOQQ_RS08400"
                     /old_locus_tag="pgaptmp_001680"
     CDS             8490..8816
                     /gene="yggL"
                     /locus_tag="ACHOQQ_RS08400"
                     /old_locus_tag="pgaptmp_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGU4469945.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            9000..9719
                     /gene="yggN"
                     /locus_tag="ACHOQQ_RS08405"
                     /old_locus_tag="pgaptmp_001681"
     CDS             9000..9719
                     /gene="yggN"
                     /locus_tag="ACHOQQ_RS08405"
                     /old_locus_tag="pgaptmp_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGU4469946.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9895..10941
                     /gene="ansB"
                     /locus_tag="ACHOQQ_RS08410"
                     /old_locus_tag="pgaptmp_001682"
     CDS             9895..10941
                     /gene="ansB"
                     /locus_tag="ACHOQQ_RS08410"
                     /old_locus_tag="pgaptmp_001682"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGU4469947.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11058..12065
                     /gene="yggM"
                     /locus_tag="ACHOQQ_RS08415"
                     /old_locus_tag="pgaptmp_001683"
     CDS             11058..12065
                     /gene="yggM"
                     /locus_tag="ACHOQQ_RS08415"
                     /old_locus_tag="pgaptmp_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGU4469948.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINKACDRWKKDTEQLRRNGVI
                     K"
     gene            complement(12221..13357)
                     /gene="hemW"
                     /locus_tag="ACHOQQ_RS08420"
                     /old_locus_tag="pgaptmp_001684"
     CDS             complement(12221..13357)
                     /gene="hemW"
                     /locus_tag="ACHOQQ_RS08420"
                     /old_locus_tag="pgaptmp_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGU4469949.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(13350..13943)
                     /gene="rdgB"
                     /locus_tag="ACHOQQ_RS08425"
                     /old_locus_tag="pgaptmp_001685"
     CDS             complement(13350..13943)
                     /gene="rdgB"
                     /locus_tag="ACHOQQ_RS08425"
                     /old_locus_tag="pgaptmp_001685"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HGU4469950.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREQKSAISHRGQALKLLLDALRNG"
     gene            complement(13951..14241)
                     /gene="yggU"
                     /locus_tag="ACHOQQ_RS08430"
                     /old_locus_tag="pgaptmp_001686"
     CDS             complement(13951..14241)
                     /gene="yggU"
                     /locus_tag="ACHOQQ_RS08430"
                     /old_locus_tag="pgaptmp_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGU4469951.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(14238..14804)
                     /gene="yggT"
                     /locus_tag="ACHOQQ_RS08435"
                     /old_locus_tag="pgaptmp_001687"
     CDS             complement(14238..14804)
                     /gene="yggT"
                     /locus_tag="ACHOQQ_RS08435"
                     /old_locus_tag="pgaptmp_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGU4469952.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(14822..15526)
                     /gene="yggS"
                     /locus_tag="ACHOQQ_RS08440"
                     /old_locus_tag="pgaptmp_001688"
     CDS             complement(14822..15526)
                     /gene="yggS"
                     /locus_tag="ACHOQQ_RS08440"
                     /old_locus_tag="pgaptmp_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGU4469953.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            15544..16524
                     /gene="yggR"
                     /locus_tag="ACHOQQ_RS08445"
                     /old_locus_tag="pgaptmp_001689"
     CDS             15544..16524
                     /gene="yggR"
                     /locus_tag="ACHOQQ_RS08445"
                     /old_locus_tag="pgaptmp_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGU4469954.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRQGISLALRLLPSHYPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(16708..17124)
                     /gene="ruvX"
                     /locus_tag="ACHOQQ_RS08450"
                     /old_locus_tag="pgaptmp_001690"
     CDS             complement(16708..17124)
                     /gene="ruvX"
                     /locus_tag="ACHOQQ_RS08450"
                     /old_locus_tag="pgaptmp_001690"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGU4469955.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(17124..17687)
                     /gene="yqgE"
                     /locus_tag="ACHOQQ_RS08455"
                     /old_locus_tag="pgaptmp_001691"
     CDS             complement(17124..17687)
                     /gene="yqgE"
                     /locus_tag="ACHOQQ_RS08455"
                     /old_locus_tag="pgaptmp_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGU4469956.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(17796..18746)
                     /gene="gshB"
                     /locus_tag="ACHOQQ_RS08460"
                     /old_locus_tag="pgaptmp_001692"
     CDS             complement(17796..18746)
                     /gene="gshB"
                     /locus_tag="ACHOQQ_RS08460"
                     /old_locus_tag="pgaptmp_001692"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGU4469957.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(18759..19490)
                     /gene="rsmE"
                     /locus_tag="ACHOQQ_RS08465"
                     /old_locus_tag="pgaptmp_001693"
     CDS             complement(18759..19490)
                     /gene="rsmE"
                     /locus_tag="ACHOQQ_RS08465"
                     /old_locus_tag="pgaptmp_001693"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGU4469958.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(19570..20277)
                     /gene="endA"
                     /locus_tag="ACHOQQ_RS08470"
                     /old_locus_tag="pgaptmp_001694"
     CDS             complement(19570..20277)
                     /gene="endA"
                     /locus_tag="ACHOQQ_RS08470"
                     /old_locus_tag="pgaptmp_001694"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGU4469959.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(20372..20869)
                     /gene="yggI"
                     /locus_tag="ACHOQQ_RS08475"
                     /old_locus_tag="pgaptmp_001695"
     CDS             complement(20372..20869)
                     /gene="yggI"
                     /locus_tag="ACHOQQ_RS08475"
                     /old_locus_tag="pgaptmp_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGU4469960.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(20946..22340)
                     /gene="galP"
                     /locus_tag="ACHOQQ_RS08480"
                     /old_locus_tag="pgaptmp_001696"
     CDS             complement(20946..22340)
                     /gene="galP"
                     /locus_tag="ACHOQQ_RS08480"
                     /old_locus_tag="pgaptmp_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGU4469961.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5525 a         5702 c         5898 g         5309 t
ORIGIN      
        1 atgtaagcat acagtgttaa cgagaacagg gcgtctccgg aaaagacacc ctgtttgagg
       61 atacgatacc aaactcaatc agtgtaacca caacttacac aaacggagac cctttgatgc
      121 cgcatcctgt attaaccgcc cgtcaaggat ctcatccctt aaccagcgtg ttctggtata
      181 tggtgcccgg aggcggaatc gaaccaccga cacggggatt ttcaatcccc tgctctaccg
      241 actgagctat ccgggcaacg gggcgcatta aacctgattc gcctcgtctc gtcaatcaaa
      301 tttcttcaat ttactgcaga ctgcacaatc tatcatcact ttgccgctat tgcacgcatt
      361 gtcaggcaaa ccaggcggtc caggctgcgg aaagcggcat tgccagaagg agtgctggaa
      421 gcatgttgac caccgggaac attttaatgc cacagatgcg taatccggtt gccagcagca
      481 ataaaccgcc tacggcgctg aagtctgcca tcatcgacgg tgtggtcagc ggtaatatca
      541 gcgcggcagc ccacgccagc gttaactgga tgatcagtaa tgggatacta attaccgaca
      601 ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa gaaatcaaga aatgacttgg
      661 cgattaaaat actcggatcg ccggtcatcc cttcgttcat cgccccgaag atcccggtgc
      721 cgctggcgca aaacaggaca ataatcgcga cataattctg aataaaaggt tcatgcgctg
      781 gcttcttacg tgagtgacga aacagatttt gtgctttggc gaccgctgta ttgacgcctt
      841 tctccagcag acaaatttcg ccgattaatg ctccgagtaa ggttgctaaa accatcgctg
      901 gaaggttggc gcatttcacc accagtaaaa taccaatccc cagcgatgcc agaccaaaaa
      961 ttgatgtcat ggagacgcgg atacgttccg gtaagcgttg gctaagcagt gcaccgagca
     1021 tgccacccag taatattgca cttgcgttga taaaagggcc gatgaccaca agagttcctg
     1081 ttaactgtat tgtttgattt tcttattatg aactttttgc cttgttgatg gcttgtctgg
     1141 tcgcaaagtc ctggcttgca cgtcttagcg aaaggtgcca tgattgcgcg aattttctcc
     1201 tctctgtacg gagtttgccc gatgcacgcc atctccttac attctctcgc ttatcgccgt
     1261 ttcgcgcgaa acccttctct ttttatgcta ctgctcatgc ggtgaagtta acgcacgttc
     1321 actgcaggac aacagtaaaa tcagagcgtt tctgctttta ctgatgtctg gcggtcggag
     1381 ctggtgacca gtttgaccca tatctcatgg ggcagggttt tacaccttgt ccggtattct
     1441 tacttccccg aaacgggttt tgcgcttatg aaatcaatga atattgccgc cagtagtgaa
     1501 ctggtatccc gactttctac tcatcgccgc gtggtggcgt tgggagatac tgattttacg
     1561 gacgtcgcgg cagtcgtcat taccgctgcg gatagtcgca gtggcattct tacgttgctt
     1621 aagcgcaccg gttttcatct accggtgttt ttgtattccg aacatgctgt tgaattacct
     1681 gcgggcgtta cggcggtaat caacggcaac gagcagcatt ggctggagct ggaatccgca
     1741 gcctgtcagt atgaagagaa tttgctgcca ccgttttatg acacgctgac gcagtacgtt
     1801 gagatgggca acagcacctt tgcttgccct gggcatcaac atggtgcgtt ttttaaaaag
     1861 catcctgccg gacgccattt ttacgatttc tttggtgaga acgtctttcg cgccgatatg
     1921 tgtaatgctg acgtaaaatt gggcgatctg cttattcatg aaggatcggc gaaagatgcg
     1981 cagaaattcg cagccaaagt ctttcatgcc gataaaacct attttgtact gaacggcaca
     2041 tcggcagcga ataaagtggt gacgaatgcg ctattaacgc gtggcgatct ggtgctcttc
     2101 gaccgtaaca accataagtc gaatcatcac ggcgcgctga ttcaggcggg ggcgacgccg
     2161 gtctatctgg aagcttcacg caacccgttt ggtttcattg gcggtattga tgcgcactgt
     2221 tttaatgaag agtatctgcg ccagcaaatt cgcgacgttg cgccagaaaa agccgacctg
     2281 ccgcgcccgt ttcgcctggc gattattcag ctgggaacct atgacggcac tgtctataac
     2341 gcccgtcagg tgatcgatac cattgggcat ctgtgtgatt acattctgtt tgattccgcg
     2401 tgggtcggtt acgaacagtt tatcccgatg atggcggata gctcgccgct gctgttagaa
     2461 cttaacgaaa acgatccagg gatctttgtg actcagtcgg tgcacaaaca gcaggcggga
     2521 ttctcacaga cgtcgcagat ccataaaaaa gataaccata tccgcggaca ggcgcgtttt
     2581 tgcccgcata agcggttgaa taacgccttt atgctccatg cttctaccag ccctttctat
     2641 ccgctgtttg ctgcactgga tgttaacgcc aaaattcatg aaggggagag tgggcgtcgg
     2701 ctgtgggctg agtgcgttga gttgggtatt gagtcgcgca aggctattct tgcgcgctgt
     2761 aagctgttcc gcccgtttat cccgcccgtt gttgatggca aattgtggca ggattatccg
     2821 acatcagtgt tagccagcga ccgccgtttt ttcagttttg agccgggggc gaagtggcac
     2881 ggctttgaag gatatgccgc ggatcagtat tttgttgatc cgtgcaagct gttactcact
     2941 acaccaggta tcgatgccga aaccggcgag tatagcgact ttggcgttcc ggcgacgatt
     3001 ctggcgcact atctgcgtga gaacggcatt gtgccggaga agtgcgatct caactccatt
     3061 ctgttcttat taactccggc ggaaagccaa gagaagctgg cacaactggt ggcgatgctg
     3121 gcgcaatttg aacagcatat tgaggatgac tcgccgctgg ctgaggtgtt gccgagcgtt
     3181 tataacaaat atccggtgcg ctatcgcgac tacaccctgc gccagttgtg tcaggagatg
     3241 cacgatctgt atgtcagttt cgacgtcaaa gacctacaaa aagcgatgtt ccgccagcag
     3301 agtttcccgt cagtggtgat gaacccccag gatgcgcata gcgcttatat tcgcggtgac
     3361 gtggagttgg tgcggattcg tgatgccgaa gggcgaattg cggtagaagg ggcgttgcct
     3421 tatccacctg gcgtgctttg cgtggtaccc ggggaagtct ggggtggggc ggttcaacgt
     3481 tatttccttg cactggaaga aggggtgaat ttgttgccgg gattttcgcc ggagctgcaa
     3541 ggtgtttata gcgaaaccga tgcggatggc atgaaacggt tgtacggtta tgtgttgaag
     3601 taagaataaa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt aattagtggc
     3661 taaccgtctg tgtacctgtc gggacacgaa cgtgtttata tttgaacatc gccatgaacg
     3721 cgaaggccag aaccacggag taaccagcga aaatcaacca tacggtctgc cagtcggtaa
     3781 tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg aggatacagc
     3841 cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt gccgggctaa
     3901 cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg caaccgtaaa
     3961 cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg taagcaaaca
     4021 gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta ataccgtagc
     4081 ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc gaaatcgaca
     4141 tgatgattga cgcatgctgc acaataaagc tgctggcaaa catcggatct ttgtcgaagc
     4201 tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc agcagcattg
     4261 agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca tcgaggccca
     4321 gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc ggcagagtca
     4381 gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc atgtggctta
     4441 attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg gtgccccaga
     4501 tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg cgatagtaag
     4561 agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc aatatcacaa
     4621 ggaacatcgc ctccggcgtc gtgacctgtg ccgccatgaa cagcgtgata gcgccaatgg
     4681 tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca atccccagca
     4741 gcgcaggcat aaagaccgcg gcgataccca gtgagctata aactgcgcca atagaagcac
     4801 cgtcaaactt cagggtaaca aacatatagg aaccgagggt cgtcagccaa cttccccaca
     4861 gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg ttaatttcct
     4921 cacatcgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag atgatgggat
     4981 tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca aacattccta
     5041 atttgcaaaa cgaaattgtg atatggataa catttttgcc cctgagcatc gtcaggggcg
     5101 gttagtggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta taaagataac
     5161 ggcgagattc cgcagaggga tggcgggtcg tcagcgtttg ataaacatcg cccggcgtca
     5221 tggtgttaat aatattagcc gcctgaatct tatcattcga aaagactcgc agcacgctgc
     5281 ctgcgccgcc gttataggcg gtgatgacgg cataacgccg ggatgtaggg ttatcaattc
     5341 cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca atattgctgg
     5401 caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag cggaacacat
     5461 ctttcccggc agtatgttgt accacctgca ttaaccccag cgcatcggaa cggctgaccg
     5521 catacgggtt aaaggaagat tcagtctgca taattgccag aatcagcgac tcatcaacgc
     5581 catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt ttatcaaggt
     5641 ggttcggcac catgttaatg gtgacgctgt agatgatacg cagcccgttg ctgcggctct
     5701 tcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc cagcgaatcg
     5761 gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa atcgtaatat
     5821 catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcaat aacgttttga
     5881 taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatggtttcg atagtaatcg
     5941 taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat ttcacgtagt
     6001 ccttaggacc agcgatcacc acctctttga agccccagat gttctcaata ttgtgggcaa
     6061 attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg ttataggtat
     6121 cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc agcgcgagat
     6181 attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagctgc cggaggcgtg
     6241 ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg cacctcttta
     6301 ccctcgaaca ggaagttgac catctcctgc tccagcagct tgcggtgctc ggcattcatc
     6361 atgttgagtt tcttttcatt aatcagcatg gtttgtttgt gctgccactg cgcccaggct
     6421 tctttggaga tctcgttata gatgcgtttt cccagttcgc cggggtacag ctgaaaatcc
     6481 tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat aaatcatcct
     6541 ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa caaacgctcc
     6601 acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag cgcattgcct
     6661 tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat atctaagtgg
     6721 aaatggctga aggtatgccg aaacgcggtc agctgcgtca ggttatcggc agcaatctgc
     6781 cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa acagtataaa
     6841 ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc gtgctgtaac
     6901 aggagaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc cggataaagc
     6961 gaccagctat tgttggtggc ggcaatacat ccgttttgta gcggacagag cgaacatttc
     7021 ggcttcgaac gcgtgcaaat catcgcgccc aaatccatca tcgcctgatt aaaccgttcc
     7081 acgccgaccg cgggcgtcac ctgctcgctt aaactccata atttattctc gacctctttt
     7141 ttcccaggcc agccgcttac agcatagcag cgcgccagca cccgtttgac gttaccgtcg
     7201 agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt ggaacgcccg
     7261 acgcccggca gtgctgcaac ttcctcaaag gtttccggga atttaccgcc gtgtaaggtc
     7321 gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc aagccccgtc
     7381 cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt cgggaagcgc
     7441 gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg caacatcact
     7501 tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagagt ttttcgcccg
     7561 tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat ggtcaccgaa
     7621 ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa ccggaacttt
     7681 ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa tgcccgtttt
     7741 cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc accggaattt
     7801 gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca ggggcgactg
     7861 accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt tgagttcagc
     7921 gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac gcttgagatt
     7981 ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccccga gcaggacttc
     8041 ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc gcatgaagag
     8101 ggcttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca taaaatgatt
     8161 cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca caaagcgcgc
     8221 cataataaac gccgtatcgt tcaggtgccg tttgccgaac tggtaaaaag caaactgcag
     8281 ctggggggcg tattccatat ggcgaccgac tgggaacctt atgcggaaca tatgcttgaa
     8341 gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta cgtaccgcgt
     8401 ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg tcacggagta
     8461 tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt cgtctgcgta
     8521 aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg cgattcccgg
     8581 aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac gaggttatcg
     8641 aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt ctgatctgca
     8701 tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag tggctggaag
     8761 agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg gactaagaaa
     8821 gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca tgcctacaaa
     8881 gatgcacgat cgagtaggcg ggataaggcg tttacgccgc atccggcatg gaaaacgcgt
     8941 actttgttat caatctgggg ccagcaaatg ctggcctgat ttgttcttga gggaagacta
     9001 tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc gctcacgccg
     9061 actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa accgtgcagg
     9121 tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg tataacggta
     9181 agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag gctgaactac
     9241 gtagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgagaaa gcccgtattg
     9301 ctctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc agccgtctga
     9361 ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg cgcagcgatg
     9421 gcctgacgtt ccactataaa gccattgatc aggttcgcgc tgaaggccag caattagtga
     9481 atcaggcaat gggcggaatt ttacaggaca gcattaatga aatgggcgcg aaagcggtgc
     9541 tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg ctgcaatcct
     9601 caatccaaac cgagtggaaa aagcaggaaa aagatttcca gcagtttggc aaagatgttt
     9661 gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat ttaaaataat
     9721 cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt tttgttacct
     9781 gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta acgtcaaatt
     9841 tcccatacaa agctaaggga taatgcgtag cgttcacgta actggaggaa tgaaatggag
     9901 tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc agcattggca
     9961 ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg tgactccgca
    10021 accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa tgcggtgccg
    10081 caactgaagg acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg ctcccaggac
    10141 atgaacgatg atgtctggct gacactggcg aaaaaaatta acaccgactg cgataaaacc
    10201 gacggcttcg tcattactca cggtaccgac acgatggaag aaaccgctta cttcctcgac
    10261 ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc gtccacctcc
    10321 atgagcgcag acggtccatt caacctgtat aacgcggtag tgactgcagc tgataaagcc
    10381 tccgctaatc gtggcgtgct ggtagtgatg aacgacaccg tgctggatgg tcgtgacgtc
    10441 accaaaacca acaccaccga cgtagcaacc tttaagtctg ttaactacgg tccgctggga
    10501 tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca caccagcgac
    10561 acgccgttcg atgtctctaa gctgaatgaa ctgccgaaag tcggcattgt ttataactac
    10621 gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga tggcatcgtc
    10681 agcgctggtg tgggtaacgg caacctgtat aaaaccgtat ttgacaccct tgcaaccgcc
    10741 gcgaaaaacg gcactgcagt cgtgcgttct tcccgcgtac cgacgggcgc taccactcag
    10801 gatgccgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct gaacccgcaa
    10861 aaagcgcgcg ttctgctgca actggctctg acgcaaacca aagatccgca gcagatccag
    10921 cagatcttca atcagtacta atcgcctcgc cccggtatcg tgccggggct ttttcacttc
    10981 agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa taaattattc
    11041 cgcgcgaggg ttttcgggtg aaaaagcaat ggattgttgg cacggcgctg cttatgttga
    11101 tgactggtaa tgtctgggca gatggcgaac cgccaactga aaatatctta aaagatcaat
    11161 tcaaaaagca gtatcacggc attctcaaac ttgatgccat caccttaaaa aatcttgatg
    11221 ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt gacgatctct
    11281 atacctgggt cggtcagttg gcagattacg aactgctgga acagacctgg acgaaagata
    11341 aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct ggctggtcgg
    11401 tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc gacgatatca
    11461 aaacgaataa taaatatctg atcgtgaata gcgaagattt caattatcgc tttagtcagc
    11521 ttgagtctgc gttgaataac cagaagaact cgattcctgc gctggaaaag gaagtgaaag
    11581 cgcttgataa gcaaatggtg gcggcgcaga aagcagcgga tgcatattgg ggaaaagatg
    11641 caaacggtaa gcaaatgacc cgcgaagacg cctttaaaaa aattcaccaa cagcgtgatg
    11701 agtttaataa acagaacgat agcgaggcgt tcgcggttaa atatgacaaa gaggtttatc
    11761 aaccggcgat tgcggcatgt cataaacaga gtgaagagtg ttatgaagtg ccgattcagc
    11821 agaaacgaga tttcgatatc aacgaacaac ggcgacagac ttttctgcaa tcacaaaaac
    11881 ttagccgtaa attgcaggat gactggataa cgctcgaaaa agggcaatat ccgttaacga
    11941 tgaaagtttc agaaataaac agtaaaaaag tcgcgatttt gatgaagatt gacgatatca
    12001 ataaggcctg cgatcgctgg aaaaaagata ccgaacaact gcggcgcaat ggggtgatta
    12061 agtaagtttt gatatagcgc acgatttgta gcaggatgcg gcgttgaccc cgcatccggc
    12121 aatcaacgtc tgatgcgacg ctagcgcgtc ttatcagacc tacaaccgtt gccacatgta
    12181 ggctggataa ggcgttcacg ccgcatccgg caatacaagt ttactcagcc agaaaaagct
    12241 ccagcagcga atttaaaaac aacttcccat gttccgttat ctgccagtaa tccgcacatt
    12301 ctgtgagata accctgggca atcgcctcgt ctaactgtgg gcgaatcaca tcttcgcaaa
    12361 gcccggtata cgcactaaac tctgcgcgtg gcgcggcctc cagcaagcga aagcggttca
    12421 taaagaactc aaacggctta tctgcagctt cgacatcacg ctggctttcc agataccttc
    12481 cttgcataaa accacgcgga tgacgcgttt tggtggtacg cagaatgcgc ccatccggga
    12541 aggtcacttt gccgtgcgcg ccgcagccaa taccgatgta gtccccaaag cgccagtagt
    12601 tgagattgtg ctggcactgg taaccgggtt tggcgtaagc ggaagtttca tattgctgat
    12661 aacccgctgc ggttaataac tgatgcccct gttcgaatat atcccacaac gcgtcatcgt
    12721 ccggcagtac aggagggcgc gagccaaaca gcgtattggg ttcgatggtc agttgatacc
    12781 aggaaagatg cggcggattc agttcaatgg cctggcgcag atcgccaagc gcctcttcca
    12841 gtgattgatc cggcagccca tgcatcaaat caaggttaaa actacgcagc cccagcccgc
    12901 ttgccagatt cgcagctcgt ttcgcttctt gcgggccatg aatgcgccca agtcgtttca
    12961 gcttttcttc gctaaaactc tgtacgccaa tagagatgcg gttcacgcca gcacgctgat
    13021 aatcgacaaa gcgatcggct tctaccgtac cagggttcgc ttccatagta atttctgcat
    13081 ccgctgccag cggcaaacgc gcacgcacgc cgtccagcag cgtttgcatc gccgggccgg
    13141 aaagcaggct cggcgtacca ccgccaataa aaattgtctg cacttcacgg ccctgagcgt
    13201 aagccacatc gttgtccaga tcgttaagca gatgctgaac ataatcgtcg tgcggcactt
    13261 ctcctttcaa cgcgtgagag ttgaaatcgc agtacgggca tttctgcacg caccacggga
    13321 tgtgaatgta gagactcagc ggcggtaatt taaccattac gtaaagcgtc cagcagcagt
    13381 ttcaacgcct gaccacggtg ggaaatggcg ctcttttgtt cgcgagtcag ttcggcagcg
    13441 gttttccctt cggaaggtac gaagaagatt ggatcataac caaagccacc agtgcccgcc
    13501 ggttcacgag taatcactcc tggccagcta ccgtggcaca ccagcggagt cggatcttcc
    13561 gcgtgacgca gatacaccag cacgcagtgg aaacgcgcct gacgttggtc gtccggtacg
    13621 tctttcagtg tttccagcag tttttgcaga ttcttttgat cggtcgcgtc ttcaccggaa
    13681 taacgcgcgg agtaaatgcc tggcgcgcca ccaagtacat ctaccgccag accggagtcg
    13741 tcggcaattg caggtaaacc ggtcactttt gccgcatggc gcgctttcag aatcgcgttt
    13801 tcgataaagg tcaggccggt ttcttcagcg gaatctacgc cgaggtctgt ttgggccacg
    13861 atatcaagac cgaagtcgct aagcagcgac gccagctcac gcactttacc ggcattgccg
    13921 gttgcgagga caactttttg cataggatac ctaattaatt aacgccgcga tttctggcgg
    13981 gatttgttgc ggattaatga ttttaatttg tttgtggcgg ccaagttcgc ctttttcaat
    14041 caccacctgg cttttggcaa cccggaattg cttaccgaga aacttcacca gatgactgtt
    14101 ggcctggccg tcaaccggcg gcgcggttat ggcgactttt acctcgtcgc catgtaaacc
    14161 gacaatagaa tcacggctgg ctttcggctg aatataaagc cgtaaaacca gaccgtcatc
    14221 gttaactgtt acggcactca taacgccatc cacagccccg gcagcagcat gtttccggtt
    14281 gcctgtaata cttctgcgac acccatattg atgacataca gcagcagaac gaggatcatc
    14341 ggcgagaaat caatcccacc cattgccggt agcaggcggc gaatcgggcg cagcagcgga
    14401 tcggccagct gaatcagcac gtattcaatc gggctacggc cctggcttac ccagctcata
    14461 atcgccatca ccagcaggac ccagaaaatc agcaggccga tggttttcag cagaatcagt
    14521 aaaccggcaa tccagatgat tggcaggaag gtcaccactt taaacagcac gatggctttg
    14581 ataaaactga gaatataggc aaccagtagc gaggcgctat caatggggcc cattgccgga
    14641 ataacgcggc gcagtggccc gataattggc tgcgttacct tcactacaaa ctgtgagaag
    14701 gggttgtaaa aatcacaatg agcccactgc atccagatgc gtaataacag caccatggta
    14761 tacagctcaa tgaccgttga aagcaggaaa gtcaacgtat tcatggcgtt cctttaattc
    14821 cttatttttt agagtaatca cgcgcaccaa aaattgcagt gccgatacga accatcgtgc
    14881 tacctgccgc aatggcggct tccatatcgt ccgacattcc cagagagagc gtgtcgatat
    14941 gcgggtagcg cgttttcagt ccggcaaatg ctacagccat ttggcgtgca acttcaaact
    15001 gccttacata ttctgactca ggcgcaggga ttgccatcag cccgcgcaga cgtaaacgcg
    15061 gtagttcagc gaccgctgct gccagctcgt ccagttctgc cagttgaatc ccggacttac
    15121 tgttttcatc actaatatta atttgaatca gcacgttaag agggggaagt tctgccgggc
    15181 gctgatcgtt gaggcgggta gcgatgcgca aacggtcgat ggtatgacac cagtcgaaat
    15241 gctctgccac caggcggctt ttattagact gcaacgggcc aataaaatgc cattctaatc
    15301 ctgttacgcc cagttcctga aagtggcgaa ttttatctac cccttcctga acgtagtttt
    15361 caccaaattg acgctgcccg gcatcaatgg cttctgcgat ggcgctcgca ggttttgttt
    15421 tactgactgc aagcagcgta atttcttctg gagaacggcc gcaacgcgtt gcagccgctg
    15481 agattttgtc ccggacctgt gccaggttat gcgcaatatc gttcattttc cgaggatctt
    15541 agtatgaata tggaagaaat tgtggccctt agtgtaaagc ataacgtctc ggatctacac
    15601 ctgtgcagcg cctggcccgc acgatggcgc attcgcgggc taatggaagc tgcgccgttt
    15661 gatgcgccgg acgtcgaaga gctactgcgg gagtggctgg atgacgatca gcgggcaata
    15721 ttgctggaga atggtcagct ggattttgcc gtgtcgctga cggaaaacca gcgattgcgt
    15781 ggcagtgcgt tcgcgcaacg gcaaggtatt tctctggcgt tacggctgtt accttcgcac
    15841 tacccgcagc tcgaacagct tggcgcacca ccggtattgc cggaattact caagagcgag
    15901 aatggcctga ttctggtgac gggggcgacg gggagcggca aatctaccac gctggcggcg
    15961 atggttggct atctcaatca acatgccgat gcgcatattc tgacgctgga agatcctgtt
    16021 gaatatctct atgccagcca gcgatgtttg atccagcagc gggaaattgg tttgcactgt
    16081 atgacgttcg catcgggatt gcgggccgca ttgcgggaag atcctgatgt gattttgctc
    16141 ggagagctgc gtgacagcga gacaatccgt ctggcgctga cggcggcaga aaccgggcat
    16201 ctggtgctgg caaccttaca tacacgtggt gccgcgcagg cagttgagcg acttgtggat
    16261 tcatttccgg cgcaggaaaa agaccccgtg cgtaatcaac tggcaggtag tttacgggca
    16321 gtgctgtcac aaaagctgga agtggataaa caggaaggac gcgtggcgct atttgaattg
    16381 ctgattaaca cacccgcggt ggggaatttg attcgcgaag ggaaaaccca ccagttaccg
    16441 catgttattc aaaccgggca gcaggtgggg atgataacgt ttcagcagag ttatcagcag
    16501 cgggtggggg aagggcgttt gtgacgttgc catgcccgat gtgcaagctt atcgggccta
    16561 caaaggcaat aaacgtctga tgcgacgctt gcgcgtctta tcagacctac aaccgctgcc
    16621 acatgtaggc tggataaggc gttcatgtcg catccggcaa taagacgcga tgatattatt
    16681 tacactccgc caggctttta aatcgcctta atatccctgc tcgaaatagc tttcgagaat
    16741 aataaccgca gaggcagagt cgactttgcc tttgttaagc gcccgatagc cgccctgttc
    16801 aaacagaccg gaacgggctt ccacggtgct aagacgctcg tcatggagct ttacttcaac
    16861 accgaaacga ccatgaatac ggttggcaaa tttacgcgct ctggcggtta acggctgttc
    16921 ggtaccgtcc atattcagcg gcaaaccaac gatgatttcg tccggctgcc actctttcag
    16981 taaacgttcg atgagattcc agtccggcgt accgtcctgt gctttaattg caggcaatgg
    17041 gcgagcggtg ccggtaatgc gttggccgac cgctacgcca atgcttttgg taccgaagtc
    17101 gaaggcgagt aaggttccac tcatcaggcg tgtcctgcca caccaggcat ggtgagaata
    17161 tccacaccaa tcagttttgc cgcctcgcgc cagcggtcgg caattggcgt tttgaacaga
    17221 atattcagat ctgccggggc ggttagccac gcgttatcga gaatttcttg ttccagttga
    17281 cctttctccc aggaggcata acccagagcc accaatacgt cagacggttg tttatcggtg
    17341 ccgagcgttt ccagcacatc gcgggaagtg gtcattaccg tgttgtctga aatgcgaatg
    17401 ctggaagcaa aattagaggg cggagtatgc aaaataaacc cgcgatcttc agccagtggg
    17461 ccgccgagca taaccggttt atccagacgg attgattcat cacgcggctc cggcgtgatc
    17521 ttcagctttt ccagaatccc ttcaattttg agattttcca gcggcttgtt gacgatgatc
    17581 cccattgcac cattggtatt atgttcgcaa atgtagacca cggaacgacg gaaaatcgga
    17641 tcctggagag caggcatggc aataagaaag tgatgctgta aattcattgt cagaggttct
    17701 gtttcctggt tcaaaaaagc aacagcgccc agtatgcgtg gaaagaaggg cgctgtcact
    17761 cagagtctca acgagatcct tctcgctaag gtgggttact gctgctgtaa acgtgcttca
    17821 atggcatcca ttaacattcc agtgatcgac accggaaact ctgcttcaat ctcacgaata
    17881 caggttgggc tggtgacgtt aatttcagtc agacggtcgc cgatgatatc cagaccaaca
    17941 aaaatcagcc ctttttcttt cagcgtcggc ccgatctgac gggcgatttt ccagtcactt
    18001 tccgtcagcg gacgaggttc accgcgacca ccggcagcca gattgccacg ggtttcgccc
    18061 ccctgcggaa tacgcgccag gcagtacggt accggctcgc catccaccac cagcacgcgt
    18121 ttgtcgccat ctttaatggc tggcaggtaa ttttgcgcca tgcagtagcg agtgccatgc
    18181 tcagtcaggg tttcggcaat cacgccgagg tttggatcgc cttctttcac gcggaaaatc
    18241 gacgcgccgc ccataccgtc cagcggctta agaatgatgt cgctgtgttt ctcccagaac
    18301 gcttttagct gcgctttatt gcgcgtaacc agcgtttctg gcgttaagtc agagaaccag
    18361 gcggtaaaca gtttctcgtt acagtcgcgc aggctctgcg gcttgttaac gatcagcgtc
    18421 cctttctctt cggcacgttc cagaatatag gtcgcgtaga taaactcggt atcaaacggc
    18481 gggtctttac gcatcaggat cacatcgaga tcggccagcg gcagatcctg ttcaccgacg
    18541 aatgaaaacc actcttcgta gttctgcttc acgttcagcg tgcgggtatg ggcgcgggct
    18601 tcgccattga tcagatacag atcgcccatc tccatatagt gaagttcgta accacgacgc
    18661 tgtgcttcca gcaacatagc gaaactggaa tctttcttga tgttgatgtt tgcgatgggg
    18721 tccatcacga tgccgagctt gatcattatt cttctccgtt agcccaaatc gccaaatcgt
    18781 acttgtagcg cggtaatggc ggtgagcgca gttgtctctg tacgcaaaac gcgaggtccc
    18841 aacaggatat cagtaaattg atagcgggca gtcatggcaa tttcatctgc cgataaaccg
    18901 ccttccgggc caatcagcag gcggacgcgt tcaaccggta aaggcaacgt attgatgctg
    18961 ttactggcgc gcgggtgaag attcagtttc agtccttcat cctgctctgc acaccaggct
    19021 tccagatcca tcgctggacg gatttccggc actcggttac gaccacactg ctcacaggca
    19081 gcaattgcaa tcttctgcca ctgctggagc ttcttgttca gacgttcact atccagttta
    19141 acgccgcagc gctcagaaaa aagtggcgta atgaggctta caccgagttc gatcgatttc
    19201 tggatcgtaa attccatttt ttcaccacgc gacatcacct gaccgaggtg aatatgcagc
    19261 ggagattcgc gatcgtcgat ctggccttcc agcaccttca cttccacgct ttttttgctg
    19321 gcgctggtaa tttcggcgtc aaagacctgg ttgctgccgt caaacaattg caacgcctgc
    19381 cccggcccca tgcgcagtac gcgcccgata tggttggcgg catcttcgca aagcgcgatg
    19441 tgagaatggc tggtcagtgg ttcaggatga taaatgcggg ggatacgcat agttaaaaat
    19501 ccgcgtcgtc tccccacgcg gttgtacgcg tggggtaggg gttaacaaaa agaatcccgc
    19561 tagtgtaggt tagctctttc gcgcctggca agcgcgttgc acatacgggt tatgattgcc
    19621 ctgcaccttc gcgatgcgtt catcgcgctc gcactcccag tcggtaaccg gatacatctt
    19681 gttccatgcg ttgaacagct gcgtttgctg gcgagagagt gtcaggttgt attggtcacg
    19741 catatagaag taggtgcgcg caatggcacc gcgtgcacgc gctggtggtt cggcagcttt
    19801 ttctttgaaa tcgaccttca tggcgcattg accgtactgg ccttcgccgc cattccactg
    19861 gctgtacata aagttgccgc gatcgccatt cacctcgccg actgacggct gcaggttatg
    19921 catatcgctt tccatcttgc gatagaccgg atctttagcg cagtttttac gtccaccgtc
    19981 ctgccagcac tggcgctggt gaccgaattg ccaggcggga acgacgtgtt cccactctac
    20041 gcggctggcg cggttttcat ttttgcgcac ctgatagccg cacgattgca gatcaacaac
    20101 gccttttttg ccctgccagt taattttaca tccgcaataa aacgtaccgg gcgcgtcagc
    20161 gtggactttt accgccgcgg ctttcgcctg agaaaaacta ttgatacctt cggccaacgc
    20221 cgggccggaa aatgctgcgc tcagtaccac cgcagcaata gacaaataac ggtacatcac
    20281 ttactccgtg ttaaaacgag ccagcaacgt agcgaaagtt gttttggtat gcaatcagcc
    20341 agatcaggaa agttcctgat agttcagatg gttattttgc gaccagttgt tcgccgcagt
    20401 gaacacagcg atagacggct tcgccacgaa ctacgcgatt atggcggcgt acggtaagct
    20461 gatgctcctg gcacttgcag cggtagggga aggtgttgcg acgcacggat tgcagttcga
    20521 actgatgcgt acgacgggct ggaacgccca gcacactttc catcatccac ttccactctt
    20581 tgccatgtgg tgcaacgcgg ccaaaatgtt tccataccag caaatgtgcc agttcgtgcg
    20641 gcaccacttc ttcaataaag gcttcactgt tttccaacag caaaacggga ttgaggcgga
    20701 tttcatagct ttccagccag gctgttccgg cggaggttcc gcgctgggtg taagagagtt
    20761 ttggctccgg gtagttacgc cctagcttca ggttggcctg ggcgagtttt tcccgcaggc
    20821 gacgcataac ggcctgttgg atggcgatag ggagacggga tgttttcata gcggcagagg
    20881 atagagcgaa gaaaagatga ctgcaagagg cggcttcctc cgcgacggga ggaagcctga
    20941 ggagattaat cgtgagcgcc gatttcgcgc agtttacgac ctttcatcag attacgttca
    21001 atatgttcca gcgaaacgtg tttggtttcc ggtaccagcc acaatgtcag caggataaac
    21061 agtacgttca gagccgcata cacccagaag gtgttggcgt tacccagcgt gttgagcatg
    21121 gtcaggaacg ttgcgccaac gatcatgttg gcaatccagt tggtggcggt ggagcaggtg
    21181 atgccaaaat cgcggccttt cagcggctga atttcggagc acagtaccca aatcagcgga
    21241 ccggcactca tggcaaaacc gacaataaac atcagcagca tggcgatggc gaaatactgc
    21301 gccgacggag agtggatacc gatatgcatc attgtaccga gtacgcccat gccagcagcc
    21361 atcaccagga agcccagcgt tagcgttggt ttacgtcccc agcggtcaac aagaccgatt
    21421 gcgataaagg tggcaagtac gttggtcagg ccgacaatca ctgtccccca catttgctcg
    21481 gtagtgttgg tataacccgc cagttcgaag attttcggcg cgtaatacat gatgacgttc
    21541 atcccggtga attgctgcat tacctgtaac agtacgccaa ggaacaccgc gcggcggaag
    21601 ttgctattct ctttaaacag cgcccagcca ctctgtttaa cctgcaaact ttcacggatt
    21661 tcatccagtt cgcgtttcgc ttccgcgctg gtgtcacgca ggcgtagcag cacgcgttcg
    21721 gcatcaacaa aacggcgttt ggcggcaaac caacgtgggc tgtctggcag gaagaagaca
    21781 ccaatcagca gcaaaattgc cgggatgata atcacaccca gcatccagcg ccatgcaccg
    21841 gtgtagctga aggcagtatc agaaagataa gcaccgagga tcccgatagt gatcatcaac
    21901 tgatacatcg agatcatact gccgcgaatt ttttccggcg caatttcaga gaggtacagt
    21961 ggtgcggtat aagaggccac acctaccgcc agccccagca gaacgcggga aagaatcagt
    22021 acttcaacgt ttggcgcagc cgcagagaac agcgaaccgg caacaaacaa aattgcgccg
    22081 atcatcaggc tctttttgcg cccgagttta aaggagagcc agccgctgcc caccgcaccg
    22141 actgccgcac cgaacatcat ggagcttacg acccattctt gcgtgtgcga agtaatctgg
    22201 aattcatctg caataaacgg cagtgcgcca gcaattacac cgatatccag gccaaagagt
    22261 aatcccgcca gagcggcaag gaagcagacg aaaaacgtca ttgccttgtt tgaccgcccc
    22321 tgttttttag cgtcaggcat gatgccctcc aatatggtta ttttttattg tgaattaaga
    22381 taggtgagta cgacgtaaaa agatgtgaag caaatcacat cagtgtaatc gctt
//
LOCUS       DAZKFU020000055        22420 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZKFU020000055 DAZKFU020000000
VERSION     DAZKFU020000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.055"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..235
                     /gene="xisR"
                     /locus_tag="ACHOQQ_RS08485"
                     /old_locus_tag="pgaptmp_001697"
     CDS             2..235
                     /gene="xisR"
                     /locus_tag="ACHOQQ_RS08485"
                     /old_locus_tag="pgaptmp_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4469962.1"
                     /translation="MTPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEYRHYAAEG
                     EPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            269..1585
                     /locus_tag="ACHOQQ_RS08490"
                     /old_locus_tag="pgaptmp_001698"
     CDS             269..1585
                     /locus_tag="ACHOQQ_RS08490"
                     /old_locus_tag="pgaptmp_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4469963.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKAYNIFLKSNS"
     gene            1603..2253
                     /gene="rcsB"
                     /locus_tag="ACHOQQ_RS08495"
                     /old_locus_tag="pgaptmp_001699"
     CDS             1603..2253
                     /gene="rcsB"
                     /locus_tag="ACHOQQ_RS08495"
                     /old_locus_tag="pgaptmp_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGU4469964.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(2453..5302)
                     /gene="rcsC"
                     /locus_tag="ACHOQQ_RS08500"
                     /old_locus_tag="pgaptmp_001700"
     CDS             complement(2453..5302)
                     /gene="rcsC"
                     /locus_tag="ACHOQQ_RS08500"
                     /old_locus_tag="pgaptmp_001700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGU4469965.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(5577..6353)
                     /gene="yfaP"
                     /locus_tag="ACHOQQ_RS08505"
                     /old_locus_tag="pgaptmp_001701"
     CDS             complement(5577..6353)
                     /gene="yfaP"
                     /locus_tag="ACHOQQ_RS08505"
                     /old_locus_tag="pgaptmp_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGU4469966.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIHGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(6358..8007)
                     /gene="yfaQ"
                     /locus_tag="ACHOQQ_RS08510"
                     /old_locus_tag="pgaptmp_001702"
     CDS             complement(6358..8007)
                     /gene="yfaQ"
                     /locus_tag="ACHOQQ_RS08510"
                     /old_locus_tag="pgaptmp_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HGU4469967.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLNEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQAMAQAGQGDRYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(8008..12522)
                     /locus_tag="ACHOQQ_RS08515"
                     /old_locus_tag="pgaptmp_001703"
     CDS             complement(8008..12522)
                     /locus_tag="ACHOQQ_RS08515"
                     /old_locus_tag="pgaptmp_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HGU4469968.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLDGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVTLNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWITL
                     QRLNDTQYEARVPVNSTFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSLLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEAPRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLTHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(12546..13169)
                     /gene="yfaT"
                     /locus_tag="ACHOQQ_RS08520"
                     /old_locus_tag="pgaptmp_001704"
     CDS             complement(12546..13169)
                     /gene="yfaT"
                     /locus_tag="ACHOQQ_RS08520"
                     /old_locus_tag="pgaptmp_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HGU4469969.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGNAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(13166..14854)
                     /gene="yfaA"
                     /locus_tag="ACHOQQ_RS08525"
                     /old_locus_tag="pgaptmp_001705"
     CDS             complement(13166..14854)
                     /gene="yfaA"
                     /locus_tag="ACHOQQ_RS08525"
                     /old_locus_tag="pgaptmp_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HGU4469970.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNNETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DEEQDTEATAIASDLLSGKKRWQVSFGLEERTAGKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFLALNDESASVDNSFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(15003..17630)
                     /gene="gyrA"
                     /locus_tag="ACHOQQ_RS08530"
                     /old_locus_tag="pgaptmp_001706"
     CDS             complement(15003..17630)
                     /gene="gyrA"
                     /locus_tag="ACHOQQ_RS08530"
                     /old_locus_tag="pgaptmp_001706"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGU4469971.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            17777..18499
                     /gene="ubiG"
                     /locus_tag="ACHOQQ_RS08535"
                     /old_locus_tag="pgaptmp_001707"
     CDS             17777..18499
                     /gene="ubiG"
                     /locus_tag="ACHOQQ_RS08535"
                     /old_locus_tag="pgaptmp_001707"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGU4469972.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(18640..22392)
                     /gene="yfaL"
                     /locus_tag="ACHOQQ_RS08540"
                     /old_locus_tag="pgaptmp_001708"
     CDS             complement(18640..22392)
                     /gene="yfaL"
                     /locus_tag="ACHOQQ_RS08540"
                     /old_locus_tag="pgaptmp_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGU4469973.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDTTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIELRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFEHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFETGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALDASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     TGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSASDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIEVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFRGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         5414 a         6125 c         5463 g         5418 t
ORIGIN      
        1 gataacgccc agcaaatggt gcacagaaag aacccttatt gctgcaactg gactgaaatc
       61 aggaaccatt gccagagcaa gaaaaaaatc ctggttactg gggaaagaat accgtcatta
      121 cgcagcagaa ggagagcccg ggcctttcag tgaatgtgta tacaacattg aggaaattat
      181 gcgctggatc gaaaaccaga aacaaccagg tgccaaaaat gcaagttccg gttaacctgt
      241 taatgctcct ggacgtctgg gaggtttaat gagtaacgca tcatacccga caggcgttga
      301 aaaccatggc ggatcactcc gcatatggtt tcactataac ggcaaacgtg tcagagaaaa
      361 cctcggtgtt cctgacaccg ccaaaaaccg gaagatcgca ggtgaacttc gtacttccgt
      421 ttgttttgca atcagaatgg ggagcttcga ctacgccgcg cagttcccta attcccctaa
      481 cctgaaacac tttggtctgg gaaaaaaaga gataaccatt aaggcacttt cggaaaaatg
      541 gcttgagctt aagaaaatgg aaataacagc aaatgccatg gtcagataca aatcagctat
      601 ccgaaattgc cttgactgcc ttggagggaa caaactggca tcatcaatta cacaggaaga
      661 tgttctcatt tttcgtaaag aattattaac gggatatcag ttgccaggaa aaaatcagag
      721 gaagcctatt aaaggtcgta gtgtacctac tgtaaatagc tatatgacaa ttatctccgg
      781 gatgttccgt tttgcggcaa acaatgggta tataggcaaa aaccctttca gtgagcttcc
      841 atcactgaaa agagcgaaaa cacctcccga tccactaaca aaagatgagt ttgccagact
      901 gattgatgcg tgccaccacc agcaaactaa aaatttctgg tcgctggctg tatataccgg
      961 gatgcggcat ggcgaactgt gtgggctggc atgggaggat atcgatctgg aagcgggtac
     1021 cttgaccgtc aaaaggaatt acactcagac aaaggaattt acactaccca aaacagatgc
     1081 aggaacaaac agggtaatac aactcataca gccagcaatt gacgtattaa aaaatcaggc
     1141 gacattaacc aggcttggta aacaatatca gatagaagta aagctgcgag agtatggccg
     1201 gacaagcact cactcatgca gctttgtttt caatcctcag atattagatc gcacaggtaa
     1261 ctcaggagtt cattatgccg taacatccat caacagaaca tgggaatctg cattaaaacg
     1321 agcaaaaatt cgccatagaa aagcgtatca gtctcggcat acttatgctt gctgggcttt
     1381 aactgccgga gcaaatccaa acttcatagc caaccagatg ggacatgcga atgcccagat
     1441 ggtttacaat gtttacggtg catggatgaa agacaataac cagtcccagg tagaagtact
     1501 aaacagaaag ctatgtactt atgtcccaca ggtgccccaa cgactaaaca aagcatataa
     1561 tatattttta aaatcaaata gttaatacat aggtacaagt acatgaacaa tatgaacgta
     1621 attattgccg atgaccatcc gatagtcttg ttcggtattc gcaaatcact tgagcaaatt
     1681 gagtgggtga atgttgtcgg cgaatttgaa gactctacag cactgatcaa caacctgccg
     1741 aaactggatg cgcatgtgtt gattaccgat ctctccatgc ctggcgataa gtacggcgat
     1801 ggcattacct taattaagta catcaagcgc catttcccaa gcctgtcgat tattgttctg
     1861 accatgaaca acaacccggc gattcttagt gcggtattgg atctggatat cgaagggatc
     1921 gtgctgaaac aaggtgcacc gaccgatctg ccgaaagccc tcgccgcgct gcagaaaggg
     1981 aagaaattta ccccggaaag cgtttctcgc ctgttggaaa aaatcagtgc tggtggttac
     2041 ggtgacaagc gtctctcgcc aaaagagagt gaagttctgc gcctgtttgc ggaaggcttc
     2101 ctggtgaccg agatcgctaa aaagctgaac cgcagtatta aaaccatcag tagccagaag
     2161 aaatctgcga tgatgaagct gggtgtcgag aacgatatcg ccctgctgaa ttatctctct
     2221 tctgtgacct taagtccggc agataaagac taatcacctg taggccagat aagacgcgtt
     2281 agtgtcttat ctggcatttg cactgaatgc cggatgcggc gtaaacgcct tatccggcct
     2341 acgattccca ttatttcaac agattacatt aaagtaggcc agataagacg cgtcagcgtc
     2401 gcatctggca tttgcactga atgccggatg cggcgtaaac gccttatccg gcctacgatt
     2461 cccgcgattt cctgaccctc tcggcatata ccgtcagcgt ctgttttatc acatccagcg
     2521 ttactggctt cgacagacag ctgtccatac cggactccag acaccgctgc ttctcttcag
     2581 ccaacgcatt agctgttact ccgattaccg gcaacgtcag tcccaactga cgaatgcgtt
     2641 gcgtcaagcg gtaaccatcc atattgggca tgttgacgtc gctaagcacg atatcaatat
     2701 gattcttgct aagtacatta agcgcatcga cgccatcatt cgcggtttta cattgatagc
     2761 ccaacgatcc caactgatct gccagcaaac gccggttaat cggatgatca tccacgacca
     2821 gaatcatcat atcgtcatta tcgctgaccg ctttgtccgt cgacggcaga gcgttagcag
     2881 gatcgtcgct ctccatctcg atcaaataaa tacgcgccaa caatgccggt agctcatgcg
     2941 gtgcagccac actgtgtacc cactcccctg gcgctttctc cagcggaata ccaatatggc
     3001 gacgacagaa ggtcactacc gctctgccct gccatttttt actcactacc tcgtcagtga
     3061 ttaacacatc ttcgggagtc ggttcctgcc cttcgtatgt tgtaacgacg atgccgctgc
     3121 gctgcaaact ggtttccagg aactgacaga gcgacgcatt gcggaccgcc agccagcagc
     3181 gtttaccact cagcccttcc acgccttttt tctgcgggta ctgagcgccg tacaacggaa
     3241 tacgcacggt aaactggctg cccattcccg gttctgaatc taccgagata tcgccgtcca
     3301 tcatgctgat cagtttttca caaatcgcca gacccagacc ggtcccctgg aagtttcgct
     3361 gtacgcccgt tccgacctgg aagaagggat caaacaagcg caccacttct ttcgccggaa
     3421 tccccacgcc ggtatcgcga acacggatag agagataatc gccatcagcg cggacatgca
     3481 aaactataca gccggtatcg gtgaatttta tggcgttact caacaggttg gagatgacct
     3541 gctgtaaacg catcgggtcg ccatttaaag ccactggcac atccggttca ataaagcagt
     3601 acaagcctaa ctgcttgcgt accaccagcg gtaaatagtt ggcggtgatg tggttcatca
     3661 cttcacgcgg tgaaaactca cgcggttcga tcttcaactg ttccgattca atcttcgaga
     3721 aatcgagaat atcgctgata attttcaaca acaggctgga agagttgttc attgccgtca
     3781 ccagacgatc gacgcctttc ggtaactctt tggtttgcaa cagatccagg ttaccgataa
     3841 tgccatacag cggcgttcgc agctcatgac tgacggtggc aaggaacatc gattttgact
     3901 ggctcgcctg ttccgctgct tgtgccatct cctgcaacga ctcttccatc ttcacgcgcg
     3961 aagaaacatc caccagcaca caaatggcca cgttttcatt acgatagcgc gaatggacga
     4021 agctgatttg cagattggta ttgttgctgg tcaggacatc aacaaaattg acttgctgcc
     4081 cacagataat ttgtgtcagt cgttggcggt cctcatgcgt aagcatattg agataggtat
     4141 gcgccagttc gttacttaaa atattgacgc catcagcggt acgcaaaatg cagataccca
     4201 ctggcgcgga ggcgacaatc ttgcgattga actgctcatg ttcttccagt cgcagggcgt
     4261 cgctttccgc cggaatgaaa atacgtcgct cgtacatccg cgcgagagta aacaatgcag
     4321 ctccggcaag cacattcagc aaaattgcgt taaggatcaa catgcgaatg cgttccagca
     4381 ccttatcaac cggcaccgaa tacacgatgc ttagcgatga gggtggcaga tttttcttca
     4441 gcaccagctc ccggaaccct tccgtatagc caaaccagga gcgttcctgc atccagcgag
     4501 gatcgccctt aattttactt tctggtccgg taagcgaaat cagggtatga ccattttcat
     4561 caagaatggt aacccccatc ggcaacgtac ccggtaagaa aaaattctcc atccggatgg
     4621 tctgctcgac acccaaaagc gcctgcaacc tgttcgccag ataaactggc gtcaacgcgt
     4681 aaaaataccc gacgccaggg cgcggacctt cgctgatcca gtagaggtta ctgccgctat
     4741 catcttgtgg tgcatttcga tatttattga tgcgttcatg caaagctttc aacgcggtat
     4801 cgcgttccac tggcatatca cgcagaccga aattggccat gcagaggtta tcgctgccga
     4861 ttaaaaatac ccggttgaga tcgtaagccg cagaaaaatt atcgcgccag tagcgcataa
     4921 accacgccaa tgactccaga gaacctcgcc aggtgttact cattgcggaa caatcagagt
     4981 cggcaaacag cggttcaaac gcaggcacat ccgcctgcgt ttctcgtcca cgcggggaaa
     5041 gcacaccgtt ttctgccgat aagcgatttt cagcgatgta cttcagctct ttcatcacat
     5101 cagaggtgcg ttgaataaag cgctgagcct gatcggaact cagattaaat tcctgacgaa
     5161 tttccgattc tcgctgatgt aacgcattaa cgatgtaaaa aacggatgaa aaagcaatca
     5221 acagccagag cactaacgcc aatgctctga acatgtagcg cgaggctttc agagttgtac
     5281 gaaaagaagc aaggtatttc aaaggggcga agctccgcct caggtgaccg atggagtgtg
     5341 gttaaggtag cggtaaaagc gtgttaccgc aatgttctgt caccattgca ataatgcgta
     5401 tcgcttagaa accattgcct taaccaggaa agccggagta aatggacagt tctcagtacg
     5461 aaaaataaga aatgaccaat ctgccgacga ttgttaatgg ttatatcctg gcgtcggcaa
     5521 atcgcccagc agtcttatct gaaaaaagaa agagccaggt tgtgtgaagg gatggatcac
     5581 cagtcaaaac ttttcaccag cgtcagctcg ccagcattac gcatcggtac aataaatgtt
     5641 tcctgtttct cattgaccga tccttcatcg gtgatcagcg tcagttgggc ggtggttaat
     5701 tccgtttcgc tgcgcccacc atagtagttg atatacacct gatagcgtcc gtgaactggc
     5761 gctggcatgg cgaaaatctc gggtccgtac cccgtcgtga catccatatc cagtgcacca
     5821 ctgtttttca gcaccgtgtt accgtaccag gcgtgttcgc catcgggcgt gacaacatgg
     5881 agatcgagat cggtattgtc cgtatcccac gagagaacca gccgtaaacg tgcacgaatc
     5941 gttcctgtac ccggcgttga gtaaaactgc attttttgtc ggctttgccc atccgggctg
     6001 atgacctgca cgctgttgct gccttcagtg aaaatataag ggcgtgcaaa tgaaccatcg
     6061 gattcaattc tctgcggcat actggcaccg ttgaccacca gccggccctg ctgaacttta
     6121 cccgccgcag cataattctt tattttgccg tggatctgcg ctgagatatt ttgatcatct
     6181 gccatgttga cggacgacgc cgggtaattg atcgattgtg aaaaactggc atcttcgccc
     6241 tctgccggat gccagcctga gagcggtgcg tcaatctcga cttcctgcac accttcactg
     6301 tgagcgacag gcgaaagcgc caccagtaac aacggaagaa aaatctttcg catagcatta
     6361 gtccattaat aattgtcggg tcagcgtttc gatatagttc tcatcaagcc cggtgggata
     6421 accatcaaag gccaggtgca gatactcatg ggttaaatcc agccgatcct gaagcgtaaa
     6481 gaagttacga ataaacagcc gtttctgctg ccgatcggta taggggaaac ctgagactaa
     6541 acgacagacc gcaaacacgt ctggttcgtt gtaccccgtc tcgccttgta atatacgccg
     6601 ccactgcggc atttttttcg ccagccaggc tttcgctttg ggtaataact gacaggttga
     6661 gcgtggcgct ccccagcggc taaggctgtt gtcgggataa gcaaacgcga gtatctggtc
     6721 gtaacgatct ccctgccccg cctgggccat ggcttgccgc caggaaagcg tcccttcggt
     6781 agcgcgactg ccgtgataat gaaccggatc gcccgcatag atgagatcct gcgtccaggc
     6841 ggtcatcgtt cgcgcccccg tggtagctgg cgaagcagaa acacgctgcg tggcgctgct
     6901 gtcggggatg gtcaggcaat cgccttcccg attggcgttt tgctgtaaaa aagtgcgtat
     6961 cgccacggtc atggctttcg ctgcctcagg tggcgtactt ttcgcctcgc gatccagaac
     7021 tctggcaacg tactcttcac gatcgagatg cgactgcaat ttcagtttgc ccttttcgtt
     7081 cagtaaagtc gtctcaccgt ggctgacaaa cgtaatgtgg ttaccattgg taaaggtcac
     7141 acgatagcgc ccattaagta cgccgggttt tactgccgtc gtactctttt ccgctgtgat
     7201 tttcttcagc ggatagcgcg caaatagctc cacctctaca cattgcccac tggcaacttg
     7261 tgtcggcact ggcaaaaccc gattaagcac cgtcgcataa cgggtcagta ccgttttgct
     7321 ggtgccactg ccagtaaccc acagcggtgt accatcggtt aaccagccag caaaaccacc
     7381 ctgacggatt tcctgtttat catcggcaaa ccagctccag gttttcaccc gtacccgact
     7441 accgagcatg gatgcgacac cgattttcgc ctcgtccagc accacatcca gtaagacttc
     7501 ctgagctttg ttttgcgcag gtaacgtcgc taatgaatca agcagcgatt taaccgttac
     7561 cgaggtttct ggcttcagcg tagtaagaga cgctaaccac gcaggtgcct gacgctgttg
     7621 ccagtattgc ccccacacat ctgcgccaat atgcaaccgc tgcggcgcaa aatagagccc
     7681 acaggaacgt accagcgcgg tatcgcgcgt tatgctctcc ccagcctgac aacaataaac
     7741 ctcttccggg gagtttccgc gacactggta gggctgctcc ggttgatggg tatcctccag
     7801 ccaggcgtaa acatacagtt tccacaaact ccctaacggc gtggtgagcg agtcaggtaa
     7861 tgctgagact ttggtcacgc ctgatgacga cagctgatac agttgatcgt gctgcgcacc
     7921 gcgcagcgcc agttgtaacg gcgtctcctc cgcaagcgtt ggctgagtaa ccaacgccaa
     7981 tagccagacg attctgcgcc agttcactta tttcacctgc atcccggtcc attcactccc
     8041 tgccgcaaca ctttgctgtg caggtgcata ggaacgcaca taacgagcag gaggcagaac
     8101 gaattgccct ttttgcgaga agcgcagcaa atggcggaaa gtgaccgttc ccgtcagttc
     8161 tttcaccggc accatatagg ccagttcgcc catttcatta cgcgcttttt ccagcaattg
     8221 cccctgctgt ttcgcggcgt tgggtttatt gactgaaatg ccccatgttg tgcgctcaac
     8281 gtcggctccc ggcgggagcg gtacttccac ctgaccatag cgcagaactg catcctgctc
     8341 gctggtaagc gtaatttcat cgagatacag cgcatcgctg tcaatctcat tgctggtcac
     8401 cggctgcaga gtaaagctca tctcttcttc accggggata agccgataca actggcgttc
     8461 aacggtcacc ggaatgttac tttgttgagc cgtttttgcc ggttcacgcc agcggacctg
     8521 cacattttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacgc cctgaccaac
     8581 ccaacgccag tattcgcccc ctccagttaa cttatgtttg gcccatgcgc ccgcaggcgc
     8641 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
     8701 gcgctcaata gtagattgct cagcggtcaa accacttaaa atagcagccg cctgggtagc
     8761 gtcgcccccg ctattgagca gcacaaccgt gtgcgtcagc ggctgattgc tgtttgccgc
     8821 tttatcccac gcatattgct gaacctgtgg catcaccgtg gacttcaact gcgctttttt
     8881 cagcaacgtc atggttaaga cgtttgccac cgcatctgcc agtggagatt ccggtgtatc
     8941 gaggatcagg ctgtcattga tatcgcgggg ggcttcctca gagaaatctt cagttttagt
     9001 tccgcgccgg gcgatagctt catccaaccc tttcaacaac gttttgcacg gcagattcat
     9061 ctcctgtgcc cacgccagca ccagcgcccg atgcaataac ggcatgttat ctgcctgctc
     9121 ggcataactg tcgagcatat gctgccagta ttccggttgt tgcgttacgc cgagcgcctg
     9181 gctggcctgc cagtcggcgt accatgccca tgccgtaagg aaggcgtcac cattgccatc
     9241 ttcaccccac caggtaaagc gcgctccggg ccccgccagt tgcatcagcc gcagacggtt
     9301 atcctgaatc atctgacgaa tgtcgttagc ggcggcactt tgatgatcgg caagcgaacg
     9361 ccaggcgaga ctgagcggga tcagacggct accggtgttg atcaccccac cccacggttc
     9421 atcgactaac gcatcaagat tgttgcggaa aatctcctgc ggcgtttcac tactttgtag
     9481 ccggatattg ctcgcctgct cgggcaacat cagcgcgtta tcgccaccac cgagcatgac
     9541 atttttctgc tgttcaacgg gccagctgtt atccacaaaa gacagttttg tgctaatgct
     9601 gtcctgcact tgcccatttt gttgcagttc tgcgcttaac aagccagatt gctgaatgtt
     9661 ctgcgccagc gaaatataat tcgccccttt gtgcagcgtc agcgtctggc gcatctcagc
     9721 gcctgcaaat ttagtcacca gcgccaccgg ttcgttatcc tgctgactga agataaacag
     9781 tcctgccgac ggtttgtcgc ccacgcgata caccgttggc atactccact tcatgtagag
     9841 atttttttcc gaacgcagat aagcacgccc ctgcccgacc agcccgtcgc cgttcatccc
     9901 acgcgcggta atgcgccagc gcgttaacga atccggcatc aggaacgtaa agtacgcttt
     9961 gccttgttta tccgttgtta gcgacggcat ccatgcagcg gtatccacct cttcacgccg
    10021 tggacgttcc agcattttta ctcgccgctc gctccggtta gtcgcgccag gcgcaacagg
    10081 ctcgctggag agcgcctggt catagctgat aaatgacagg ctggaactgg tgcgcacatt
    10141 gtttcgcccc aggggataga aaaatttgcc gatattcggc gcgatttccg gttgtagcgc
    10201 gtagatcatt tcatcgacca caccaaccgt tagctgcgca gaaaccggtt tacctttcag
    10261 caacgaggtt aattcgacgt tgaccagttc gcccggctgg tagtgggttt tatccgtttt
    10321 cacccgaata tctaactggg gaacggcgac tttgatccct gcgttctgaa aactgtactg
    10381 cccgttacgg gtgtacagca ccgaaaaagt gatgttgggc gcaaaggtac tgttcactgg
    10441 aacgcgggct tcatactggg tatcgtttaa gcgttgtagc gttatccagt ttgccgggtg
    10501 cgaaagcagc gactgctgtt ccacgcgatc gcgttccagc gtcagcaagg cttcatcaat
    10561 tggttctgga aaggtaatca acatcttcgc ggtttcgcct ggctggtaca gcgttttatc
    10621 tgccacgata tctaccgtac cagtatgcga catgctgccc ttaccgctga cggcatgact
    10681 taacccagcg agaattaagc cgtctttatc gcgtaacgtc agattgtagt tgccaggttt
    10741 atcgaaattg acggtaaagg atttgccgcc agacggtagc tctccgctat ggctcgtgcg
    10801 gtcttcgaga cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc
    10861 ataacggaac acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta
    10921 atgcgccagg ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc
    10981 gtcactggcg gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa
    11041 cgtcacatga ccgctggcgt cggacaccgt ttcactgcct tccagcgaca cggggaaacg
    11101 tccggcataa cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg
    11161 cacgcgggca ttttttaccg gctcgccatc cgggtagaga agttgcagtt tgccgctgac
    11221 cgcttcgcca gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt
    11281 gatatagttg gctacgcgaa aactactgct atagacctga ttgcggtaag caagacgtaa
    11341 ctcataacct ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg
    11401 cgcatccagc gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag
    11461 cttcgccggg gcgctgacga tgggggatga atgcaacgga tcgtggaact cgcggcccat
    11521 cactttaaca tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa
    11581 gcgggtgttg tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc
    11641 agcatcctta cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaggtacc
    11701 gctgtcatcg gtcacaccgc gggtcatcac gccaagaccg tcagtccaca agatctctga
    11761 gccgggcttc gcttcaccct gttttttacc cgcggtccac accagaagct ctttgcctga
    11821 cactttgcta agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc
    11881 aaccatcgcc tcgacgaggt acagtcccgg ctcttgttgg ccgagaggaa tataaatgtt
    11941 acccggctgc ggcgagatga aattgctgga tgcgccgtcc agttttaccc cttgctgcgg
    12001 ctcgaccggt ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt
    12061 cagcggggaa aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag
    12121 atgtaattcg ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg
    12181 catcacgcgg cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag
    12241 cccgtcgccc aaatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa
    12301 aaatgccatc gggtcaggaa tacgatacag gcgaacgtcc acgccgccgt actcttccat
    12361 ctgatagcgc cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct
    12421 gctaaaactg ctgtcagcaa gcaaaaagaa tgttccccct gcgggcggcg catagttact
    12481 ggaaggaagc gaatcatcag cattagcaag ccctgttccc acaagagaca gggacaagca
    12541 cgctgctatc gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg
    12601 ttgggtatcc atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt
    12661 ccgttgtcag ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc
    12721 attaagtgct gggcattgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt
    12781 atgtcctggc caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg
    12841 ccggttttcc cgttcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc
    12901 gtcatctctg gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg
    12961 tgaactttca gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac
    13021 cagcgtggac ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga
    13081 aacagcccgg attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag
    13141 caaatcagcg ccagcagccc gtgcctcata gcggctgcca ggttatgggt agccactgcc
    13201 aggcggcacc gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag
    13261 cgtccagctt cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct
    13321 tcggcagact ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat
    13381 agagcggaac gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca
    13441 gtgtttgcag ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt
    13501 gcatcgccag cgacacgcgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct
    13561 gcgctttcgg atactgaccg tatcgggaac tcacttcgcg acgccagatt tgcgcttcgc
    13621 cctgctgagt ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa
    13681 tattttgcgt aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac
    13741 cgacaaacag cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt
    13801 ctaacgcccc attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa
    13861 taccgtgtga atacggcacc gccacacaga agctggcccc ggcaggcata ctgttccaga
    13921 ccggcgtaaa atcgaaactg ttatctacgc tggcggattc atcatttaac gccagaaagc
    13981 tatgccagcc gtcgttgctc atttcgaaac gcactccggc aaaagaaggc attaatcgct
    14041 ggtagccaaa acccagcaac ctggcgctga ccacgatgcg ctggcgtact ggtgtttttc
    14101 cggcagtacg ctcttccagg ccaaagctga cttgccagcg ttttttaccg ctcaacaaat
    14161 cacttgcgat cgccgtagct tcggtatcct gctcttcatc tttaaacagc atatccgtgc
    14221 tggaaaacac cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat
    14281 agcgcaactg ataaacagga atagtttcat tatttagctt gatgctactg atttccgttt
    14341 tacttaactg gctgtcgctg gtagcggcaa acagcaatgg ctccagcagc ttgcttaagc
    14401 cactacgctg gataagcacc atataatgtg aaagatggcc ttgtttatcg tgccatagcg
    14461 ccgcctgtgc gggctgatct aagagtgacg aaaagagttt atctttcagc gtgagatcgt
    14521 gttcatagac aatgcgacga atgctgcctt caatgcccag acgatcggca tgattctgat
    14581 aatagaaaac gaaatcttca ctcagaacat cgtggagaaa cggaatggtg aggagatctt
    14641 tgggaagctg gctcagagag tcgctgtcga gaaagaggtc cggctcattg agatcgattt
    14701 gcagattgtt gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt
    14761 gcaacgccca gacgccagcg gaaagcagta ctattgcgcc aaaacctaca agaccataga
    14821 accgccagcc tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc
    14881 cattttttac gggcacagcc aaactttacc gtgccctaat acgacaaaag cccagacttt
    14941 gcagcctgga cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa
    15001 aattattctt cttctggctc gtcgtcaacg tccacttccg gagcgatttc gtcgtctcct
    15061 tccgcagcac tgccatcgat ggtgtccaga tcttcctcgt caaccggttc agccacgcgt
    15121 tgcagaccca ccacgttttc atcttccgca gtacggatga ggattacgcc ctgggtgtta
    15181 cggcccacga tgctgatttc cgaaacgcga gtacgtacca gcgtaccggc atcggtgatc
    15241 atcatgatct ggtcgcagtc atctacctgc accgcgccaa caactaaacc gttacgttcg
    15301 gtaaccttga tggagataac ccctttcgtc gcacgcgact tagtcgggta ttccgccact
    15361 gcggtacgtt taccataacc gttctgcgtt gcggtgagga ttgcgccatc gccacgaggc
    15421 acgatcagag agacgacttt atcaccttcg cctaaacgaa taccgcgaac accggtggtg
    15481 ttgcagccca tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag
    15541 aacagcatta cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca
    15601 accagtttga tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt
    15661 ttcttcacag taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc
    15721 accggcagga tcgcggtgat acgttcgtcc tgctccagcg gcagcaggtt gacgatcgga
    15781 cgaccgcgcg cgccacgagt ggcttccggc aactgataaa ccttcatcga atagacgcga
    15841 ccacggctgg agaagcacag gatatgatcg tgagtgttcg ccaccagcag tcggtcgata
    15901 aagtcttctt ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg
    15961 tattcagaaa gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct
    16021 tcctgggtga tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga
    16081 cgtttgtcac cgaactgttc acgaaccagc tccagctctt cacggatcac ttccatcaga
    16141 cgatcggcgc taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat
    16201 tcgtcgagca gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc
    16261 gcctgagctt gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc
    16321 agccattccg gacgcgcagc atcgtcgcca gcacgttcga gcatcgcggc aacgttgccc
    16381 agctgccacg gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg
    16441 atcagttcga tgatcgggtc gatgttcgcc agcgccacgg ctaatgcttc aaggatatga
    16501 gcacgatcgc gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg
    16561 tgacgaacaa acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc
    16621 aatgccacca tgtttatacc gaaagaaacc tgcaactggg tctgggagta gaggttgtta
    16681 agcacaactt caccgaccgc gtcgcgtttc acttcaatca cgatgcgcat accgtctttg
    16741 tcagactcgt cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca
    16801 atcttctcga tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt
    16861 tcacgtccgg ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg
    16921 cgaccggtac ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg
    16981 aagtccggcc ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata
    17041 tacgccagac aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata
    17101 cctacggcga taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg
    17161 tcgggaattt tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga
    17221 tcggccatca gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc
    17281 gcagagtcgc cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc
    17341 gagaatggct gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg
    17401 tatttaccga ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca
    17461 ttgcctagta cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg
    17521 acatctggca gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttc
    17581 agctcttcct caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta
    17641 tccctctact gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagattgag
    17701 gtaaacctat acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta
    17761 cggagtagaa gtgccaatga atgccgaaaa atcgccggta aaccataacg tagaccacga
    17821 agagatcgct aaatttgaag ccgtcgcctc ccgctggtgg gatctggaag gtgagttcaa
    17881 accgctgcac cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggctt
    17941 atttggcaaa aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc
    18001 gcgcgaaggt gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa
    18061 actgcacgcg ctggaaagcg gcattcaagt ggattacgtg caagaaactg tggaagagca
    18121 cgcggcaaaa catgccgggc agtatgacgt ggtgacctgc atggagatgc tggagcacgt
    18181 ccccgatccg cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccgg gcggcgatgt
    18241 ctttttctcg acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga
    18301 atatattttg cgcatggtgc ccaaaggcac gcatgatgtg aagaagttta ttaaaccggc
    18361 agagctgctt ggctgggtag atcagacaag ccttaaagag cgacatataa ccgggctaca
    18421 ttacaacccg atcactaata cttttaaact cggccccggc gtggatgtga actatatgct
    18481 gcacacgcag aataagtgag gttgttgttt ggattcatcc ggcaaccgcg ccaatgcctg
    18541 atgcgacgct tgccgcgtct tatcaggcct acaaatgctc tccgtaggcc agataaggcg
    18601 tttacgccgc atccggcaat agtgatggtc agacagcgat taccatttca ccgtcatcga
    18661 caaaaaccct gccgtctggg caaaatcatc actccctttc tgccacgcca cgctgccgcg
    18721 cagggacact cgctgactga tattgcccgt gactcccact tttatttcac cccgttgctt
    18781 caccgcatcg tcactgatag tgctgccatc ctcttcaatt tccgtcgaat ggggatcgtg
    18841 ataataattc agatctaatg ttggtatgac atgaacagcg gtacgccatt cgctgtgtaa
    18901 acccagccgc gtctgaatat catcaccctg cgattgtgac acgcgcgcac ggttagcggc
    18961 ggtaaaatca tcctgctgca cgccctgata aatcacctgc gcctgcggtt caatcaccac
    19021 accacgcccc ggtaaccact gataccccgc ttccagcgag gcgataatcc ccgacgaatg
    19081 gtaatgatcc acgccatctt catgttcaga aacatcattg ctaaaccacg cgtactgcaa
    19141 ccagttatcc agccaggccc cctgcttctg attaccgtgc tgaaaccagc ttgaggttag
    19201 cccaacggca taaccgtggt tctggttatc ggcgcgagtt ccggtcatac tcgagcggct
    19261 gtcgccctgg ttatcgctgt agccaccaac aatcccaagc atccactcgc catccgtgcc
    19321 ccagcgcccc ctaaacaggt caccgctaag ctgcaccgta gaagtgtctt catgctgagc
    19381 cagttgccct gccgctgtgt aatgataatc tccgccgata acacgtaaat tcagcgtctg
    19441 accatcgcca cctgcgtgat cgcgtcgctc catcataaac gcctgatttg ccgcccgcag
    19501 gttattgaga taaccgccaa ctttggcatt caacaccggc tggtaagcag gcgtaggttc
    19561 cgggtcgggt gttggatcag gatccggcgt gggatcagga tcgggagtcg ggtctggatc
    19621 aggtggcgat ggcggcgtta cttcttgcga tcgcagatac cagtcgttgt tatcttccac
    19681 cagcgtgtag tcatacgctc ccatattgac gtagccgctg cctgccagac tgaactgcgc
    19741 attgttttga aactgcgtgg gatcagctgc gaaatcaacc acttcaatgc ctgtcgatgt
    19801 cggctcacca atccctgtaa tggagttaac caccacagtt gtgttgccag cagtattacc
    19861 gttcaacacc aactggtcgc ttgctgagtc atcgccgttt aattcgctgt cgaacagtaa
    19921 cgtaccgccg ccggtgtaat caccgtttac cgtcagcgta tcgccagcga cgccattttg
    19981 caggctgacc gcaccggtcg tattggtcag attgccatta actgttacgt cggaaagata
    20041 agtcaggctg tcagtttcaa ggttggtatt aagctcacct gtcaacgtca cgccatcggt
    20101 ggcattcacc agcgtaccat cgccgttaaa cagtgaggca tcaagggcga cggaagtatc
    20161 ctgcccggtc aggcgcaaaa ccgtaccatc gctgatgtcg atagtgccgc tggaagtagc
    20221 atcgatagac tgaatatcct gatccgcgcc agtctcgaac gttgccccgt caccaaccca
    20281 cagtgatgaa gcataaggaa ggatatcagc aacatcacct ttcagggtgc cttcttcgac
    20341 acgtaccgcc gactgcgttg taccgctggc ggtcagctcc agcgtacccg cccccgtttt
    20401 cgtcaatgtg ctaccctcat cctgatgctg cccgctgctg tcagccatca gtgcgcccca
    20461 ctgcgtgtct acccccgcat caactgccac ttcaccgtcg gcgcgcattt caatatcacg
    20521 cccatgctca gtcgttgtag agcgagtatc gacattggca gtaatctcca tcacgctttc
    20581 ttgtggctta tcggtaaaga taacctggcg gttataatgg gtatctccaa gctgcgagtt
    20641 gtcgctcacc atcagcgtac cggaagcgat ttgcgttgtg ccgaggtatt gattgtcatt
    20701 tgccaggctg acctgcccgt cacccgcatc gatcaccacg tagacgccat ctttcccgtc
    20761 gccgccgata tgcatatcgt ttccgcctgc aagatctacc acatcctgac tgccgataac
    20821 cgttccgctg ctgccactga cttcaaggcc atctttgtat gatgtaccgc tctgccaggt
    20881 ggagaaatca gagagatcga gcacaccgcc gtttaacacg atcgactgcg gatcgtcctg
    20941 gagagcggca agatctgccg cgtcgccttc cagcgaaagc accgcaccag catccaccac
    21001 tatatcgccg gttagcgcca tcgactgcgc ccccgccagc acatagctgc cgttttgcgc
    21061 aatggtgagc tgaccagcac cttcgatggt gccagcaaaa ctgccttgat taacagtaac
    21121 attgccacca gcatcgatat ttaaagtgcc attctgaaag cccgtcaatg agtgctcaaa
    21181 ggtttgttgg gtggagccaa catttagctc tgcctgattc tggtacttat caatactccc
    21241 tatcgtcaga ccgtagcagt cttgcggatc gtcctggcaa tgcgtatcgc cgacattcat
    21301 cagggagttg ctgcggccca gggtaacttc accgttttca atctgcatct caccagtaaa
    21361 gtcattgtta tctgcattaa gtaccagatc gccggaacct gttttggtga ttaacccggt
    21421 accagcaata gagtcaacag ctccgtcatt ctctgtattg ccaataacca gcgtttttcc
    21481 gtcggcaata tcaaatgtaa cttcacttaa gccgagatac ataaagccac ccgccgcaga
    21541 ggaaggacca tctccatagc ctgctgcgct attgttctca tcgactaaca cgcctccgtt
    21601 ctggctgtag ctgtcatcaa cagaaatatc aataagatag ggagccgtcg cgctattggt
    21661 atatatcgcc ccgccataac cttcggcagt gttatttgta aaggcagtgt tatttattat
    21721 cgtataacct gaaggatgct tgctgtcgct attattatct gtgacatcga ttgcgccgcc
    21781 atcgccatca ctgtaacttg ttgatgtata tgcctggtta ttgttaaaaa catcatcact
    21841 taaatagata tcattattga tggtataaat tgcaccacct ttgccgtcat tagcgatgtt
    21901 attgcgaaac acggcgttag tgacacgtaa ctcgatggca ccggtatcgt tagtaccaga
    21961 agaatagatt gcgccaccat agccgcctgc gacgttacca gaaaaaataa catccgtaag
    22021 attcagcgtt gagttttctt tagcaaatat tgccccgccg ttattatatt ctcctgaaac
    22081 ggtgttattg gcaaacagag tcattccagt ttcattttct ggtgataatg taaattccgc
    22141 tccttgttgc aaaaatacgg ccccaccgct ggcattattg gtcgtatccg aaaaaaccag
    22201 ccattgccca tcggcgatac tccagacctg cgtaatgcct gaaaggcttt gcctgctggc
    22261 gtgacaactc gctttgatat catatccctg gcataaatca atggccgccg cgacaccgtt
    22321 agcagagaaa agagatgcaa tcattgacgg gagtagagat aaatactcct tgcgtagaaa
    22381 gataatccgc atattaatct gaccatcatt ttatataaga
//
LOCUS       DAZKFU020000056        22261 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZKFU020000056 DAZKFU020000000
VERSION     DAZKFU020000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.056"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..398)
                     /gene="uspD"
                     /locus_tag="ACHOQQ_RS08545"
                     /old_locus_tag="pgaptmp_001709"
     CDS             complement(<1..398)
                     /gene="uspD"
                     /locus_tag="ACHOQQ_RS08545"
                     /old_locus_tag="pgaptmp_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGU4469974.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSA"
     gene            complement(425..724)
                     /gene="yiiS"
                     /locus_tag="ACHOQQ_RS08550"
                     /old_locus_tag="pgaptmp_001710"
     CDS             complement(425..724)
                     /gene="yiiS"
                     /locus_tag="ACHOQQ_RS08550"
                     /old_locus_tag="pgaptmp_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGU4469975.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(936..1376)
                     /gene="yiiR"
                     /locus_tag="ACHOQQ_RS08555"
                     /old_locus_tag="pgaptmp_001711"
     CDS             complement(936..1376)
                     /gene="yiiR"
                     /locus_tag="ACHOQQ_RS08555"
                     /old_locus_tag="pgaptmp_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4469976.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            1477..2076
                     /gene="yiiQ"
                     /locus_tag="ACHOQQ_RS08560"
                     /old_locus_tag="pgaptmp_001712"
     CDS             1477..2076
                     /gene="yiiQ"
                     /locus_tag="ACHOQQ_RS08560"
                     /old_locus_tag="pgaptmp_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGU4469977.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            2184..2951
                     /gene="tpiA"
                     /locus_tag="ACHOQQ_RS08565"
                     /old_locus_tag="pgaptmp_001713"
     CDS             2184..2951
                     /gene="tpiA"
                     /locus_tag="ACHOQQ_RS08565"
                     /old_locus_tag="pgaptmp_001713"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGU4469978.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(3006..3761)
                     /gene="cdh"
                     /locus_tag="ACHOQQ_RS08570"
                     /old_locus_tag="pgaptmp_001714"
     CDS             complement(3006..3761)
                     /gene="cdh"
                     /locus_tag="ACHOQQ_RS08570"
                     /old_locus_tag="pgaptmp_001714"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGU4469979.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESVTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(3868..4857)
                     /gene="sbp"
                     /locus_tag="ACHOQQ_RS08575"
                     /old_locus_tag="pgaptmp_001715"
     CDS             complement(3868..4857)
                     /gene="sbp"
                     /locus_tag="ACHOQQ_RS08575"
                     /old_locus_tag="pgaptmp_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGU4469980.1"
                     /translation="MNKWGVGLTFLLATTSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(5176..6138)
                     /gene="pfkA"
                     /locus_tag="ACHOQQ_RS08580"
                     /old_locus_tag="pgaptmp_001716"
     CDS             complement(5176..6138)
                     /gene="pfkA"
                     /locus_tag="ACHOQQ_RS08580"
                     /old_locus_tag="pgaptmp_001716"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGU4469981.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(6319..7221)
                     /gene="fieF"
                     /locus_tag="ACHOQQ_RS08585"
                     /old_locus_tag="pgaptmp_001717"
     CDS             complement(6319..7221)
                     /gene="fieF"
                     /locus_tag="ACHOQQ_RS08585"
                     /old_locus_tag="pgaptmp_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGU4469982.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(7370..7870)
                     /gene="cpxP"
                     /locus_tag="ACHOQQ_RS08590"
                     /old_locus_tag="pgaptmp_001718"
     CDS             complement(7370..7870)
                     /gene="cpxP"
                     /locus_tag="ACHOQQ_RS08590"
                     /old_locus_tag="pgaptmp_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGU4469983.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            8020..8718
                     /gene="cpxR"
                     /locus_tag="ACHOQQ_RS08595"
                     /old_locus_tag="pgaptmp_001719"
     CDS             8020..8718
                     /gene="cpxR"
                     /locus_tag="ACHOQQ_RS08595"
                     /old_locus_tag="pgaptmp_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGU4469984.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            8715..10088
                     /gene="cpxA"
                     /locus_tag="ACHOQQ_RS08600"
                     /old_locus_tag="pgaptmp_001720"
     CDS             8715..10088
                     /gene="cpxA"
                     /locus_tag="ACHOQQ_RS08600"
                     /old_locus_tag="pgaptmp_001720"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGU4469985.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETLKANQLWGEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(10194..10868)
                     /gene="yiiM"
                     /locus_tag="ACHOQQ_RS08605"
                     /old_locus_tag="pgaptmp_001721"
     CDS             complement(10194..10868)
                     /gene="yiiM"
                     /locus_tag="ACHOQQ_RS08605"
                     /old_locus_tag="pgaptmp_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGU4469986.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENISTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(11017..12000)
                     /gene="kdgT"
                     /locus_tag="ACHOQQ_RS08610"
                     /old_locus_tag="pgaptmp_001722"
     CDS             complement(11017..12000)
                     /gene="kdgT"
                     /locus_tag="ACHOQQ_RS08610"
                     /old_locus_tag="pgaptmp_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGU4469987.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(12260..12880)
                     /gene="sodA"
                     /locus_tag="ACHOQQ_RS08615"
                     /old_locus_tag="pgaptmp_001723"
     CDS             complement(12260..12880)
                     /gene="sodA"
                     /locus_tag="ACHOQQ_RS08615"
                     /old_locus_tag="pgaptmp_001723"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGU4469988.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            13165..14199
                     /gene="rhaT"
                     /locus_tag="ACHOQQ_RS08620"
                     /old_locus_tag="pgaptmp_001724"
     CDS             13165..14199
                     /gene="rhaT"
                     /locus_tag="ACHOQQ_RS08620"
                     /old_locus_tag="pgaptmp_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HGU4469989.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLISTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(14196..15044)
                     /gene="rhaR"
                     /locus_tag="ACHOQQ_RS08625"
                     /old_locus_tag="pgaptmp_001725"
     CDS             complement(14196..15044)
                     /gene="rhaR"
                     /locus_tag="ACHOQQ_RS08625"
                     /old_locus_tag="pgaptmp_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGU4469990.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(15118..15954)
                     /gene="rhaS"
                     /locus_tag="ACHOQQ_RS08630"
                     /old_locus_tag="pgaptmp_001726"
     CDS             complement(15118..15954)
                     /gene="rhaS"
                     /locus_tag="ACHOQQ_RS08630"
                     /old_locus_tag="pgaptmp_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HGU4469991.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            16242..17711
                     /gene="rhaB"
                     /locus_tag="ACHOQQ_RS08635"
                     /old_locus_tag="pgaptmp_001727"
     CDS             16242..17711
                     /gene="rhaB"
                     /locus_tag="ACHOQQ_RS08635"
                     /old_locus_tag="pgaptmp_001727"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGU4469992.1"
                     /translation="MTFRNCVAVDLGASSGRVMLTRYERECRSLTLREIHRFNNGLHS
                     QNGYITWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMTQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            17708..18967
                     /gene="rhaA"
                     /locus_tag="ACHOQQ_RS08640"
                     /old_locus_tag="pgaptmp_001728"
     CDS             17708..18967
                     /gene="rhaA"
                     /locus_tag="ACHOQQ_RS08640"
                     /old_locus_tag="pgaptmp_001728"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGU4469993.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
     gene            19510..20334
                     /gene="rhaD"
                     /locus_tag="ACHOQQ_RS08645"
                     /old_locus_tag="pgaptmp_001729"
     CDS             19510..20334
                     /gene="rhaD"
                     /locus_tag="ACHOQQ_RS08645"
                     /old_locus_tag="pgaptmp_001729"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGU4469994.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAHEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            20344..20658
                     /gene="rhaM"
                     /locus_tag="ACHOQQ_RS08650"
                     /old_locus_tag="pgaptmp_001730"
     CDS             20344..20658
                     /gene="rhaM"
                     /locus_tag="ACHOQQ_RS08650"
                     /old_locus_tag="pgaptmp_001730"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGU4469995.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(20699..22093)
                     /locus_tag="ACHOQQ_RS08655"
                     /old_locus_tag="pgaptmp_001731"
     CDS             complement(20699..22093)
                     /locus_tag="ACHOQQ_RS08655"
                     /old_locus_tag="pgaptmp_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGU4469996.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKAAQGNTPVNGQTTAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT         5534 a         5796 c         5530 g         5401 t
ORIGIN      
        1 tccgcactca acttattgat catcccgcga tatgccggca tcaaacggtt gataaatgag
       61 tggtgatgac cacagacaag gaggtcgcac tgctcttttt gcataatttc cagcagtgtt
      121 tccggcattt ctccgcgttc aatacgcagt tttgtcttcg gccattgaat atttttcgtc
      181 agtttataca gcttgttatc cgacttattc ttcaacaatt gaagaatatc ttctgttgca
      241 gggaagtaga tacccgggta caactcgctt aagccatcat caatatgaat taacgtcagg
      301 tgagcgtcat tatgtctggc gagctccagg gctttattca ccagtaaggc atcttcttca
      361 ttcccggaaa ttgccacgcc aatgtgttta taagccatgt ttaactcctt ctaaagcaac
      421 tccatcaagc taacgaacgc agtgataact caaaaataat catctcggcc tggcatgaga
      481 aggtgaaggc cgcatcaagc tcaacttgcc cctccagatc cttaatctta taggccacgc
      541 tggccccttc atctgcagag gatgcagcgg cggcaagctc tttcagtttg gtcataaagg
      601 actctgcttc ttcgcgggtg gcaaaaaagc gcgaaaactt actggtacag ttgtcgttat
      661 caatcaccgt accgatatca atcgcacatc ctttagtact gcatttatct acgacatctt
      721 tcatagggca cctctgtata ttcgcctctc tgtcgattac gacttaagaa taatgcgccg
      781 ttaagggaag aacaaacaag taatgatctt atggattacc attttttttg atattgctta
      841 aggtgaagag ttcagaagga ttttccgggc attgtcattt gcatcgtgga aataacaatc
      901 ccggaaaaaa atagcagtaa gagatatatt aaaggttaat tacttgattt attgtcggct
      961 ttatacttca catcctgagt atctttacca tatttatttt cgccttgtgt gccaacaaac
     1021 gcaccaagat cgataagcat catcaccaga atcaacgtcg ggacaaaacg ccctaccgcc
     1081 cattgccaga cacccggtaa aatcgcccag ttacccgcca gtagcatcca cgccacaatc
     1141 atcagaaatg cccatgcgcc ggaacgcccg cgatcatgca agcgcttaac ggttactgcc
     1201 gctgttggcc agagcaagca cacaaggcaa aacgccgcgg tctgaatatc gagtaaattt
     1261 ttacccgcca gtgagaaaag caccagcatg cctgcgaacc acaggcctat ccaaatccag
     1321 aaatcacggc gtccaatacg ccctttaaat gagaataacc attgctgtat ggtcatgtaa
     1381 gttccttgat ggttgtcttt tccaggattc tacccttttg acaagggcga cagatatcgt
     1441 tttaatcgga gccagtcata acaaaaggta ctgtcaatga agccagggtg tacgctgttt
     1501 tttctcttat gttctgcatt aaccgttaca acaacggcgc atgcacaaac accagatacg
     1561 gcaacgaccg cgccttatct gctggctgga gcccctactt tcgatctctc catcagccag
     1621 tttcgagaag actttaacag ccagaatccc agcctgccac tgaacgaatt tcgtgccatc
     1681 gacagcagtc ccgacaaagc caatctcact cgtgctgcaa gtaaaattaa cgagaacttg
     1741 tatgcttcta cagcgctgga gcgcggtacc ttaaaaatca aaagcattca aatgacctgg
     1801 ctacccatcc aggggccaga gcaaaaagcc gcgaaagcga aagctcagga atatatggca
     1861 gcggtgatcc gcacactcac cccattaatg accaaaacac aaagccagaa aaaactgcag
     1921 tcgctactaa cggcggggaa aaacaaacgt tattacaccg agacagaagg tgcactgcgt
     1981 tatgttgtcg cggacaacgg cgaaaagggg ctgaccttcg ctgttgaacc gattaagctg
     2041 gcgctatctg aatcgcttga aggtttgaat aaatgacaaa aagcaaagcc tttgtgccga
     2101 tgaatctcta tactgtttca cagacctgct gccctgcggg gcggccatct tcctttattc
     2161 gcttataagc gtggagaatt aaaatgcgac atcctttagt gatgggtaac tggaaactga
     2221 acggcagccg ccacatggtt cacgagctgg tttctaacct gcgtaaagag ctggcaggtg
     2281 ttgctggctg tgcggttgca atcgcaccac cggaaatgta catcgatatg gcgaagcgcg
     2341 aagctgaagg cagccacatc atgctgggtg cgcaaaacgt ggacctgaac ctgtccggcg
     2401 cattcaccgg tgaaacctct gctgctatgc tgaaagacat cggcgcacag tacatcatca
     2461 tcggccactc tgaacgtcgt acttaccaca aagagtctga cgaactgatc gcgaaaaaat
     2521 tcgcggtgct gaaagagcag ggtctgactc cggttctgtg catcggtgaa accgaagctg
     2581 aaaacgaagc gggcaaaact gaagaagttt gcgcacgtca gatcgacgcg gtactgaaaa
     2641 ctcagggtgc tgcggcattc gaaggtgcgg ttatcgctta cgaacctgta tgggcaatcg
     2701 gtactggcaa atctgcaact ccggctcagg cacaggctgt tcacaaattc atccgtgacc
     2761 acatcgctaa agttgacgct aacatcgctg aacaagtgat cattcagtac ggcggctctg
     2821 taaacgcgtc taacgctgca gaactgtttg ctcagccgga tatcgacggc gcgctggttg
     2881 gtggtgcttc tctgaaagct gacgccttcg cagtaatcgt taaagctgca gaagcggcta
     2941 aacaggctta agtctgacag gtgccggatt tcatatccgg cacttacttt ccttaactct
     3001 tcgccttaac gcaaaatctc acactgatga tcctgaattt cctcggctga agcacggtta
     3061 agcgtcagta gatttcgttg tgtcgccagc aatacaaatg agttatcact ctgccgtacc
     3121 atcgccagcc cgtagcttcc catatgttcc cgcgcctcag gtacttcttc tgccagcatc
     3181 ataaatgggc tgcgttgtac cagttcgctt tccgttaccc gacgcgccag gtattcatgc
     3241 ccgcgcaaac cacctggcag tggcaaccag cggctgctga tgttcgccag attgttatcc
     3301 agttgttcgc gcacatcagg acgaatacaa gagatatgaa tatgaaaatg gttttgcgta
     3361 cgcccggtgc gggagttgat cgccaaagaa accgcgcgat cgggaaccgg ctggccgtat
     3421 tttttgctca taaaatcacg cgcctgccag gccaaccaaa agaagttcgg cgttgaagga
     3481 tcggtcaaca aaggactttc agtaccgtta atacgatacg ttggcatcaa cagatattgc
     3541 agtgggccat taagatcttt taaaaccacg tatccggcat tgggtttgac ttccgcacat
     3601 ggcgaaggat tttgattttg ctgctgattg ggcaaacatt cctcaaggac aatcttacgt
     3661 aatgtaaccg actcttcacc ggttaatttc cagtaaccaa taccggcagc cacaacggcg
     3721 ataactatca tcaccaaaaa aagaagaccc gcttttttca tctttttttc cctgtacctc
     3781 aaagagatgc aagggtaacg caaaatcgtg acaaataaaa aacccggtcg cgagaaacca
     3841 ccgggtttga taattatcct ggagaaatca gcgtttgctg atctgatcga acgtaccgcc
     3901 gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg aactcttcat caatggtgaa
     3961 taacttcagc tttggaaacg cattttcgta ctttttcgcc acctcagcgt cgcgcggacg
     4021 gtagtagttt ttcgcggcaa tttcctgacc ttctggcgag tagagatatt tcaggtaggc
     4081 ttccgccacc tctttagtac cttttttctc gaccacttta tcgaccaccg acacggttgg
     4141 ctctgcgagg atagactcac tcggcgtgac gatttcgaat ttatctttcc ccagttcatt
     4201 cgctgccagc agagcttcgt tttcccaggc aatcagtaca tcgccaattc cgcgctcgac
     4261 aaaagtgtta gtggagccgc gcgcgccaga atccagaact tcgacgtttt tatacagtgc
     4321 ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg tgatgcagcg cgtagcccca
     4381 ggcagccagg taattccagc gcgcgccacc agagcttttc ggattaggcg tgatcactga
     4441 aacacccggt ttaatcagat cgttccagtc atggatctgc ttcggatttc ccttacgtac
     4501 caggaaaaca atggtggaag tgtacggtgc ggagttatcc ggcagacgtt tgatccactc
     4561 tttatcaatc cgcccgcgtt ccgcaattgc gtccacgtca taggccagag ccagcgtgac
     4621 aacatcagct tcaataccgt tgattaccga cgtcgcttgt ttacctgaac caccgtgtga
     4681 ctgacgaatc accacgttat caccagtttg ctgtttccag tgggcgctga atgccttgtt
     4741 gtactgttcg tacaattcgc gcgttggatc atatgaaacg ttaagaagct gaatatcctt
     4801 tgccataacg ctggttgtcg ccagcaaaaa tgttaaccct acgccccact tgttcatcgc
     4861 ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc agaaggttaa ccaaacatta
     4921 aagaataaaa aaagattggc tataacttgc gggtatatgt tgggggttaa aaaggcggaa
     4981 gaaatccgcc tcatattgct gacaaagtgc gctttatcga tgccggatgc ggcgcgaacg
     5041 ccttatccgg cctacaaaag tttgtaaatt caataaattg tagaattcac gtaggcctga
     5101 taagcgaagc gcatcaggca tttttgcttc tgtcatcggt ttcaggctaa aggaatctgc
     5161 ctttttccga aatcattaat acagtttttt cgcgcagtcc agccagtcgc ctttgaacgg
     5221 acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga accagctgtt cgttctggat
     5281 acctacgcaa cgaccgccgt aacctgccag cagcagatcg atagcgtaag cgcccatacg
     5341 ggaagccaga atacggtcgt aaggcaccgg agaaccaccg cgctggatgt ggcccagcac
     5401 agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc
     5461 acacatatgt tcggtaatcg ccacgatcgc gtgtttttta cctttcgcga tacccgcttt
     5521 gatttcgttt accaggtctt cacggctgaa ttcaacttcc ggaaccacaa cgaattcaca
     5581 gccaccagca atggccgcag ccagcgtcag atcgccacaa taacggccca tcacttccac
     5641 cacggaaata cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg cttctacaac
     5701 ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat
     5761 agtgccaggc agaccgatgc acgggaagcc catttcggtc agacgcattg cacccatgta
     5821 ggaaccgtca ccgccgataa ccaccagcgc atcgatccca cgttttttca ggttttcgat
     5881 agccacggcg cggatgttct cgtcgcggaa ttccgggaaa cgcgcagaac cgaggaacgt
     5941 accgccacgg ttgatcatgt cagaaacgct gtaacggtct agctgtacca tacggtcttc
     6001 atacagaccc agatagccgt cataaatgcc cattacttcc agaccttctg tcagcgcaga
     6061 acgaacaacc ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac
     6121 accgattttc ttaatcatga ctacctctga actttggaat gcaaaatgaa atctgttgcc
     6181 ggaagtcttc ttacacatcg aagtgatcca acgaatgtgc aaatagtata acaatcactt
     6241 cctgctgaat tgattcaggt caggccaaat ggcggtattt tatacacaaa atgcgggtct
     6301 ggctctcttt tatactgatt atgaaagcat agaccgttta ccctccctgg gtacgacgga
     6361 acaggggtcc tgatggataa tcacatccga tcccggaaaa cgccgtaaaa tagcctgctc
     6421 tacctgatcc gccaccatat gtgcctgaac caaaggcaga gagtcttcca tttccaaatg
     6481 aatctgaata aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag cgccgctaac
     6541 acccggccag gaagtcacga tatcaataat ttcttgccgt tcctcatcag gcaatgcgcg
     6601 atccagtaat gactgtaccg cctcatatcc catgcgtaac gcgctatata aaatatagat
     6661 gccgattccc aatgcaaaca gagcatcggc gcgatgccag ccgtaccagg acaaccccag
     6721 cgcgagcaga attgcgccgt tcatcataac atcagactgg taatgtagca tatcagcccg
     6781 caccgcctgg ctttgcgtcc ggcgcaccac ccaacgctga aacgagacaa ggataatcgt
     6841 acaaattagc gccacaattg tcacgataac cccgacgcct ggatctgtca tcggtgttgg
     6901 agatatcaga tgttgaatac ccgtcaaaaa caggaatagt gccgaaccgg agataaacat
     6961 actttgcgcc agcgccgcga gggactcagc tttaccgtga ccaaacgagt gattatcgtc
     7021 ggcaggttgc agggaatatc gcaccaccag taaattcgtc aacgacgcgc cgatatccac
     7081 cagcgaatcc accagcgcgg cgagaatact caccgacccg gtataccacc atgcaaaaat
     7141 tttaatcagc aatagcagcg aagccatcgc cgtcgcagca atcgccgccc gactgaccag
     7201 ccgtccataa gattgattca taaatactcc cgctatcaac tgacgctagt ataacggaag
     7261 caaatcatct gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa cccccacatc
     7321 atgtggggga agacagggat ggtgtctatg gcaaggaaaa cagggtttac tactgggaac
     7381 gtgagttgct actactcaat agcttcaacg atgaactttt ttgccattgc gtcacgtcgc
     7441 gcaactgctc cattcgttgt tgatgtttct cgtttaaaac cgcttgctgc tccggcgtta
     7501 acaggcgata catttggttg cggacttttg ccatctcaac ctgacgagca atttgctcat
     7561 tcgccatttt ttctgcctgt gcgcgcacag cgttttcatc aaaattttct gcggtgacaa
     7621 ggcgatgcat tgtctccagt tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt
     7681 gcataagatc tcgcatctgc tgacgctgat gttcggttaa acttatgccg tcgaacatat
     7741 ggctctgcgt actgcgctgc gtaagttctt cacccggatg ccagttatcg cctgaaccga
     7801 cttcagcagc gtggcttaat gaactgactg ccagcgttga ggccatgacg gcagcggtaa
     7861 ctatgcgcat catttgctcc caaaatcttt ctgtcgcgat tcaacgatag agagtttacg
     7921 attcaggctg caaacatgcg tcagggggtg taaaacaacg taaagtcatg gattagcgac
     7981 gtctgatgac gtaatttctg cctcggaggt atttaaacaa tgaataaaat cctgttagtt
     8041 gatgatgacc gagagctgac ttccctatta aaggagctgc tcgagatgga aggcttcaac
     8101 gtgattgttg cccacgatgg ggaacaggcg cttgatcttc tggacgacag cattgattta
     8161 cttttgcttg acgtaatgat gccgaagaaa aatggtatcg acacattaaa agcacttcgc
     8221 cagacacacc agacgcctgt cattatgttg acggcgcgcg gcagcgaact tgatcgcgtt
     8281 ctcggccttg agctgggcgc agatgactat ctcccgaaac cgtttaatga tcgtgagctg
     8341 gtggcacgta ttcgcgcgat cctgcgccgt tcgcactgga gcgagcaaca gcaaaacaac
     8401 gacaacggtt caccgacact ggaagttgat gccttagtgc tgaatccagg ccgtcaggaa
     8461 gccagcttcg acgggcaaac gctggagtta accggtactg agtttaccct gctctatttg
     8521 ctggcacagc atctgggtca ggtggtttcc cgtgaacatt taagccagga agtgctgggc
     8581 aaacgcctga cgcctttcga ccgcgctatc gatatgcaca tttccaacct gcgtcgtaaa
     8641 ctgccggatc gtaaagatgg tcacccgtgg tttaaaacct tgcgtggtcg cggctacctg
     8701 atggtttctg cttcatgata ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga
     8761 cgctggcgct ggtgttgatg ttggttttga tgttacccaa gctcgattca cgccagatga
     8821 ccgagcttct ggatagcgaa cagcgtcagg gtctgatgat tgagcagcat gttgaagcgg
     8881 agctggcgaa cgatccgccc aacgatttaa tgtggtggcg gcgtctgttc cgggcgattg
     8941 ataagtgggc accgccagga cagcgtttgt tattagtgac caccgaaggc cgcgtgatcg
     9001 gcgctgaacg cagcgaaatg cagatcattc gtaactttat tggtcaggcc gataacgccg
     9061 atcatccgca gaagaaaaag tatggccgcg tggaactggt cggtccgttc tccgtgcgtg
     9121 atggcgaaga taattaccaa ctttatctga ttcgtccggc cagcagttct caatccgatt
     9181 tcattaactt actgtttgac cgcccgctat tactgctgat tgtcaccatg ttggtcagta
     9241 cgccgctgct gttgtggttg gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg
     9301 ctgccgatga agttgcccag ggaaacttac gccagcaccc ggaactggaa gcggggccac
     9361 aggaattcct tgccgcaggt gccagtttta accagatggt caccgcgctg gagcgtatga
     9421 tgacctccca gcagcgtctg ctttctgata tctctcacga gctgcgcacg ccactgacgc
     9481 gtctgcaact gggtacggcg ttactgcgcc gtcgtagcgg tgaaagcaag gaactggagc
     9541 gtattgaaac cgaagcgcaa cgtctggaca gcatgatcaa cgatctgttg gtgatgtcac
     9601 gtaatcagca aaaaaacgcg ctggtaagcg agacattgaa agctaaccag ttgtggggcg
     9661 aagtgctgga taacgcggcg ttcgaagccg agcaaatggg caagtcgttg acagttaact
     9721 tcccgcctgg gccgtggccg ctgtacggca atccgaacgc cctggaaagt gcgctggaaa
     9781 acattgttcg taatgccctg cgttattccc atacgaagat tgaagtgggc tttgcggtag
     9841 ataaagacgg tatcaccatt acggtggacg acgatggtcc tggcgttagc ccggaagatc
     9901 gcgaacagat tttccgtccg ttctatcgga ccgatgaagc acgcgatcgt gaatctggcg
     9961 gtacaggttt ggggctggcg attgttgaaa ccgccattca gcagcatcgt ggctgggtga
    10021 aagcagaaga cagcccgctg ggcggtttac ggctggtgat ttggttgccg ctgtataagc
    10081 ggagttaaac tccgcatttg taggcaggat aaggcgttta cgccgcatcc ggcatttgag
    10141 caggatgcct gatgcgacgc tgatagcgtc ttatcaggcc tacactccgg gggttatttt
    10201 ccccacaacc ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt ttgcatcgtc
    10261 cgcgtccagc ttttcgataa cccggcggcg gagagtaaac ggtgatactg atcatcatca
    10321 aacggcatat gccatgcgat ggcggcagcc tcctgcacgg tcacatcgct gacacgggaa
    10381 accaactcca gcggcgcgtc cgcagacact ttccctggcg ctatcacgct atacaaccat
    10441 cccactttac ccgtgttttg catcaactgc gctatatcgc tgatatcaaa atggtaattg
    10501 agtttatagc aaggcgagcg cggctggctg acctgaatta atgcctctcc ccagcggaaa
    10561 atatcgccaa tataaacatt actttccgtg agaccgtcgg ttgagatatt ttcgccaaac
    10621 gcaggcgcaa caaacaactc cgcctgttcc ggaaattccc gcgcccagta gagatagtgc
    10681 tcacgaggat aatgacacag cgctctgtct ggcccgccgt gaaccttttt ctccgcctgc
    10741 tcgtcacctt ccagccccag ctccgtcagc atcaactcac catcaacctg gattttggca
    10801 attgcgctgg gtttgccttc gggataagcc tgaatcttgc ctgtgtatac atcaaccgga
    10861 tatcgcatct gttccctctc cacgagaaat ttccccatta gaccatattt attttatgtc
    10921 aggcgtgctg ttttcgcggt aagtatccag gcaggaactc aatagcaaac cggacgaacg
    10981 ttagttcatc cggctgtggg gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc
    11041 gattttcgcc gctctggctt tgactttacg tgaccagata gaggtaagaa ttggcaccag
    11101 aatcgaagtc acaatgactg ccgtcgctac cagcgaagtt gctgccggag ccatcggttt
    11161 aaacgcaggc accatttctg caatcagcac aggtgtcgct accgcggccc ctgcggaact
    11221 ggaagcggca attccggcag tgccatcgcc accgccaatc aatttatcgg cgataatcaa
    11281 cggaataccg gtcacgataa ttactgccac acccaacagg atccccagca aacctgtctg
    11341 ggcaattaca gtcaaatcaa tggtattgcc cagtgcgaag gcaaagaatg gaatcagcgt
    11401 ctgcaccgct ttgctgaaaa attctcgtaa ttcagggtca aggttcccca gggcaaagcc
    11461 caccaggaac ggtaatactg cgccgacgaa aacatgcggt tcaaacgagg caatccctgc
    11521 agtgcccaga ataatcatcg tcatgagcgg cccggactcc aacgacatca acacaaatgc
    11581 cccagcttct tcttttgtgc cgtactgctg catgatggaa gcgtaaagtc cgccgttggt
    11641 catatccatc gccgccacca gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac
    11701 accatgttcc ggaataatgc gcgaggcaat tgccgcaacc acccacgcga cggcaatttt
    11761 agttaccacc agcgtaccgg atttacgcag taccgttccc gtcgcgctta attttattga
    11821 cgcccccatg caaaaaaacc acaccgccag aatgggcacc gtaccggtaa tcatcccgtt
    11881 ggtgaatgat ccaaaatatt tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag
    11941 gaatagcggg acgagcatca tcccccccgg gattttctca atcgagcgtt ttatctgcat
    12001 ttccatcacc tgtcggcata tttttaaggg ctgatgcgta attcctcaat ggagcatcag
    12061 tgttgatgga aacgaagata gacattcatt taatcaataa aagtgatcaa aaccacatca
    12121 attgaaacac tgttttattt ttatcggatc attgttttta ttttgaaatt gatcacaaaa
    12181 aaaccaccgc cgttggcgat ggttcatttt ttaagctgat atgcggccta tacgcctcat
    12241 tgcagcaggc ggcaaatgat tattttttcg ccgcgaaacg tgccgctgct tcgtcccagt
    12301 tcaccacgtt ccagaactct ttgatgtagt ccggacggcg gttctggaat ttcaggtagt
    12361 aagcatgttc ccacacatcc aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt
    12421 cacccatcag cggagaatcc tggttagcag tagaaaccac cgccagttta tcgcctttca
    12481 gcaccagcca tgcccagccg gaaccaaagc gggaagctgc cgctttttca aattctgctt
    12541 tgaagttatc tacggaaccg aaatcacgtt cgatagccgc tttcaggtca ccctgcaggg
    12601 tggtgccttt tttcagacct ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt
    12661 tgcgcagtac ggttttcttg tctgctggca gctggtccag tttggtgatc agctcttcaa
    12721 ccggcaggtt ggcaaattct ggcaggcttt ccagcgccgc gttggcgttg ttgacgtagg
    12781 tctgatggtg tttggtgtgg tggatttcca tggtctgctt atcgaagtgc ggttccaggg
    12841 catcgtaagc atacggcagg gatggcaggg tatagctcat attcatctcc agtattgtcg
    12901 ggcggccgat tgttaatgcc gcgtaagcag ttggttcatt atagttaatt aaatgatatt
    12961 gaaaatgatt atcaatgccg tacttttcgt aagggtatgg ttttgcagga aaatgcccga
    13021 gatgtgaagc aaatcaccca cttaatgccg tgattgccag taaatcgaca acggcggcaa
    13081 caggcgaaag gttaatcgac agcacgattt ttacactcat ctcgtcggag atgtgacgcg
    13141 acgaaaaatg atgaggataa gaagatgagt aacgcgatta cgatggggat attttggcat
    13201 ttgatcggcg cggccagtgc agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg
    13261 tcatgggaaa ccatgtggtc agtcggtggg attgtttcgt ggattattct gccgtgggcc
    13321 atcagcgccc tgttactacc gaatttctgg gcatattaca gctcgtttag tctctctacg
    13381 ctgctgcctg tttttctgtt cggcgctatg tgggggatcg gtaatatcaa ctacggcctg
    13441 accatgcgtt atctcggcat gtcgatggga attggcatcg ccattggcat tacgttgatt
    13501 gtcggtacgc tgatgacgcc aattatcaac ggcaatttcg atgtgttgat tagcaccgaa
    13561 ggcggacgca tgacgttgct cggcgttctg gtggcgctga ttggcgtagg gattgtaact
    13621 cgagccgggc agttgaaaga gcgcaagatg ggcattaaag ccgaagagtt caatctgaaa
    13681 aaagggctgg tgctggcggt gatgtgcggc attttctctg ccgggatgtc ctttgcgatg
    13741 aacgccgcaa aaccgatgca tgaagccgct gccgcacttg gcgtcgatcc actgtatgtc
    13801 gctctgccaa gctatgttgt catcatgggc ggcggcgcga tcattaacct cggtttctgt
    13861 tttattcgtc tggcaaaagt gaaggatttg tcgctaaaag ccgacttctc gctggcaaaa
    13921 ccgctgatca ttcacaatgt gttactctcg gcactgggcg ggttgatgtg gtatctgcaa
    13981 ttctttttct atgcctgggg ccacgcccgc attccggcgc agtatgacta catcagttgg
    14041 atgctgcata tgagtttcta tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag
    14101 tggaacaatg caggacgccg tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt
    14161 gtcgccgcta acatcgtcgg catcggcatg gcgaattaat ctttctgcga attgagatga
    14221 cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta
    14281 tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg ctggagaaga
    14341 tattgcgcat gacacactct gacctgccgc agatattgat tgatggtcat tccagtctgc
    14401 tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaatttatcc
    14461 agcgcaaagg gacttttcag gctagccgcc aaccgggtaa tcagcttatc cagcaacgtt
    14521 tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc
    14581 aactgcccga acagcaactc tgccatttcg ttagcaaacg gcacatgctg actactttca
    14641 tgctcaagct gaccgataac ctgccgcgcc tgtgccatcc ccatgctacc taagcgccag
    14701 tgtggttgcc ctgcgctggc gctaaatccc ggaatcgccc cctgccagtc aagattcagc
    14761 ttcagccgct ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag
    14821 gagtgtttat cgtcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga
    14881 tcgttgagta catgcagacc attaccgcgc cagacaatca ccagctcaca aaaatcatgt
    14941 gtatgttcag caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg
    15001 ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc
    15061 agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta
    15121 ttgcagaaag ccatcccgtc cctgacgaat atcacgcggt gaccagttaa actctcggcg
    15181 aaaaagcgtc gaaaagtggt tactgtcgct gaatccgcaa tgataggcga tgtcagtaac
    15241 gctggcctcg ctgtgacgta gcagatgtcg agccttcatc agtcgcaggc ggttcaggta
    15301 acgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac gcagcgaaag
    15361 agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca
    15421 ggccagaagc aagttgagac gtgatgcgct gttttccagg ttctcctgca aactgctttt
    15481 acgcagcaag agcagtaatt gcataaacaa gatctcgcga ctggcggtcg agggtaaatc
    15541 attttcccct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaatacgct
    15601 gtggttaacg cgccagtgag acggatactg cccatccagc tcttgtggca gcaactgatt
    15661 cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattag tcagacacag
    15721 attatcggta tgttcataca gatgccgatc atggtcgcgc acaaaacaga ccgtgccacc
    15781 ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac
    15841 aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat
    15901 cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc
    15961 ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt
    16021 ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga
    16081 aatgcggtga gcatcacatc accacaattc agcaaattgt gaacatcatc acgttcatct
    16141 ttccctggtt gccaatggcc cattttcctg tcagtaacga gaaggtcgcg aattcaggcg
    16201 ctttttagac tggtcgtaat gaaattcagc aggatcacat tatgaccttt cgcaattgtg
    16261 tcgccgtcga tctcggcgca tccagtgggc gcgtgatgct gacgcgttac gagcgtgaat
    16321 gccgcagcct gacgctgcgc gaaatccatc gttttaacaa tgggctgcat agtcagaacg
    16381 gctatatcac ctgggatgtg gatagccttg aaagtgccat tcgccttgga ttaaacaagg
    16441 tgtgcgagga agggattcgt atcgatagca ttgggattga tacctggggc gtggactttg
    16501 tgctgctcga ccaacagggt cagcgtgtgg gcctgcccgt tgcttatcgc gacagccgca
    16561 ccaatggcct gatgacgcag gcacaacagc aactcggcaa acgcgatatt tatcagcgta
    16621 gcggcatcca gtttctgccc ttcaatacgc tttatcagtt gcgtgcgctg acggagcaac
    16681 aacctgaact tattccacac attgctcacg ctctgctgat gccggattac ttcagttatc
    16741 gcctgaccgg caagatgaac tgggaatata ccaacgccac gaccacgcaa ctggtcaata
    16801 tcaatagcga cgactgggac gagtcgctac tggcgtggag cggggccaac aaagcctggt
    16861 ttggtcgccc gacgcatccg ggtaatgtca taggtcactg gatttgcccg cagggtaatg
    16921 agattccagt ggtcgccgtt gccagccatg ataccgccag cgcggttatc gcctcgccgt
    16981 taaacggttc acgtgctgct tatctctctt ctggcacctg gtcattgatg ggcttcgaaa
    17041 gccagacgcc atttaccaat gacacggcac tggcagccaa catcaccaat gaaggcgggg
    17101 cggaaggtcg ctatcgggtg ctgaaaaata ttatgggctt atggctgctt cagcgagtgc
    17161 ttcaggagcg gcaaatcaac gatcttccgg cgcttatctc cgcgacacag gcacttccgg
    17221 cttgccgctt cattatcaat cccaatgacg atcgctttat taatcctgag acgatgtgca
    17281 gcgaaattca ggctgcgtgt cgggaaacgg cgcaaccaat cccggaaagt gatgctgaac
    17341 tggcgcgctg tattttcgac agtctggcat tgctgtatgc cgatgtgttg catgagctgg
    17401 cgcagctacg cggtgaagat ttctcgcaac tgcatattgt cggcggcggc tgccagaaca
    17461 cgctgctcaa ccagctatgt gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg
    17521 aagcctcgac gctcggcaat atcggcatcc agttaatgac gctggatgaa ctcaacaatg
    17581 tggatgattt ccgtcaggtc gtcagcacca ccgcgaatct gaccaccttt acccctaatc
    17641 ctgacagtga aattgcccac tatgtggcgc agcttcactc tacacgacag acaaaggagc
    17701 tttgcgcatg accactcaac tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc
    17761 ggtggggatt gatgtcgagg aggcgctgcg ccaacttgat cgtttacccg tttcaatgca
    17821 ctgctggcag ggcgatgatg tttccggttt tgaaaacccg gaaggttcgc tgaccggtgg
    17881 gattcaggct actggtaatt atccgggcaa agcgcgtaat gccagtgagc tacgagccga
    17941 tctggaacag gctatgcggc tgattccggg gccgaaacgg cttaatttac atgccatcta
    18001 tctggaatca gacacgccag tctcgcgcga tcagatcaaa ccagagcact tcaaaaactg
    18061 ggttgaatgg gcgaaagcca atcagctcgg tctggatttt aacccctcct gtttttcgca
    18121 tccgctaagc gccgatggct ttacgctttc ccatgccgac gacagcattc gccagttctg
    18181 gattgatcac tgcaaagcca gccgtcgcgt ttcggcctat tttggcgagc aactcggcac
    18241 accatcggtg atgaacatct ggatcccgga tggtatgaaa gatatcaccg ttgaccgtct
    18301 cgccccgcgt cagcgtctgc tggcagcact ggatgaggtg atcagcgaga agctaaaccc
    18361 tgcgcaccat atcgacgccg ttgagagcaa attgtttggt attggtgcgg agagctacac
    18421 ggttggttcc aatgagtttt acctgggtta tgccaccagc cgccagacgg cgctgtgcct
    18481 ggacgccggg catttccacc cgactgaagt gatttccgac aagatttccg ccgccatgct
    18541 gtatgtgccg cagttgctgc tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt
    18601 ggtgctgctg gatgatgaaa cccaggcgat tgccagtgag attgttcgtc acgatctgtt
    18661 tgaccgggtg catatcggcc tcgacttctt tgatgcctct atcaaccgca ttgccgcgtg
    18721 ggtcattggt acacgcaata tgaaaaaagc cctgctgcgt gcgttgctgg aacctaccgc
    18781 tgagctgcgc aagctggaag cggcgggcga ttacactgcg cgtctggcgc tactggaaga
    18841 acagaaatcg ttgccgtggc aggcggtatg ggaaatgtat tgtcagcgtc acgatacgcc
    18901 agcgggtagc gaatggctgg agaacgtgcg ggcttatgag aaagaaattt tgagtcgccg
    18961 cgggtaaaca ctgccggatg cggcgcgagc gccttatccg gcctacaggt cggcaacagt
    19021 tgtaggcctg ataagacgcg acagcgtcgc atcaggcatt gattgccgga tgcggcgcga
    19081 gcgccttatc cggcctacag gtcggcaaca gttgtaggcc tgataagacg cgacagcgtc
    19141 gcatccggca ttgattgccg gatacggcac gagtgcctta tccggcctac aggtcggcaa
    19201 tagttgtagg cctgataaga cgcgacagcg tcgcatcagg cattgattgc cggatgcggc
    19261 acgagcgcct tatccggcct acaggtcggc aatagttgta ggcctgataa gacgcgacag
    19321 cgtcgcatca ggcattgatt gccggatgcg gcacaagtgc cttatccggc ctacaggtcg
    19381 gcaatagttg taggcctgat aagacgcgac agcgtcgcat caggcattga ttgccggatg
    19441 cggcacgagc gccttatccg gcctacgggt cgtgcatccg acaacaccga atttacagga
    19501 acacagaaca tgcaaaatat tacccagtcc tggtttgtcc agggaatgat caaagccacc
    19561 accgacgcct ggctaaaagg ctgggacgag cgcaacggcg gcaacctgac gctgcgcctg
    19621 gatgacgccg atatcgcgct ttatcacgac aacttccacc cacaaccgcg ctatattccg
    19681 ctcagccagc ccatgccttt actggcaaat acgccgttta ttgtcaccgg ctccggcaaa
    19741 ttcttccgta acgtccagct tgatcctgca gctaacttag gcgtcgtaaa agtcgacagc
    19801 gacggcgcgg gctaccatat tctctggggg ttaaccaacg aagccgttcc aacttctgaa
    19861 cttccggctc actttctctc ccactgcgag cgtattaaag ccaccaacgg taaagatcgg
    19921 gtgatcatgc actgccatgc caccaacctg atcgccctca cctatgtact ggaaaacgac
    19981 accgcggtct tcactcgcca actgtgggaa ggcagcaccg agtgtctggt ggtattcccg
    20041 gatggcgttg gcattctgcc gtggatggtg cccggcacgg acgaaatcgg ccaggcaact
    20101 gcccacgaga tgcaaaaaca ttcgctggtg ttgtggccct tccacggtgt gttcggttcc
    20161 ggaccgacgc tggatgaaac attcggttta atcgacaccg cagaaaaatc agcggaagtg
    20221 ttagtgaagg tgtattcgat gggcggcatg aaacagacca tcagccgtga agagttgata
    20281 gcgctcggca agcgcttcgg cgttacacca ctagccagtg cgctggcgct gtaaggagca
    20341 aacatgatcc gcaaagcctt tgtcatgcag gttaaccccg acgcccacga agagtatcag
    20401 cgtcggcata accccatctg gccggaactg gaagcagtgt tgaaatctca cggtgcgcat
    20461 aactacgcca tttacctcga caaagcgcgt aatctgctgt ttgccatggt agagattgaa
    20521 tctgaagaac ggtggaatgc ggtagccagc actgaaatct gccaacgttg gtggaaatat
    20581 atgaccgacg ttatgcccgc taacccggat aacagcccgg tgagtagcga gctgcaagaa
    20641 gtgttttacc tgccttaatg agttgcggtg ccggaaaata tccagcaccg cgacttcatc
    20701 agaagaagta tttaaagcga acgcgtgcgc cgtaacggtc atcagtatca tcaccgtgtt
    20761 ttttatcgcc gtcgctatcc agaatggagt aatacgtccc gaggtaaata ttaaagttct
    20821 ccatatccat cacattagcg aactgataag acgcatgaat ggtgtgaatg gtgtacgtcc
    20881 cttcatcatc aatccagcag tcgttatcac aaactacacc gctgaattca tcaatcttat
    20941 tgtgcgcgta gatataaccg agctcgaaac gtttccacag cgcgttaatc ccggcagtga
    21001 aatctttctc attattagca tctaaatagg cggtattaag attaaccaca atgccattgt
    21061 ccggatccgt tttcaggccg ttccaggtca tgctcatacc atagccggta cgatcggact
    21121 gatcgacaaa attacccttg ctatcggtat aaccataagc attatttacc acgttcgctt
    21181 ccatcgctgc ggaaacggtg aattcttccg tcggcgacca ggcaattact gggcgcagat
    21241 aagcaacatt tttctgctgc tccatatcgc gtccatgata attaccgtca ttatataaag
    21301 atgttccgtc ttcaagtaac gtgtttaatt caaaatacca gttatcgagt tgtttgctga
    21361 cgaggaaatt accgccagcg ttagagcgcc cgcggccctc tttcatcata tagatataac
    21421 cgctgccatc gtcataaaga tcgttcgcag tattaccgga atgttcaaca aaggtatcct
    21481 gattcagcgg gaacatatcg taggcttcaa aacggcctac tttgaccttc cagtcgtttt
    21541 ctttgccaaa gaagaatacc gcatcatcga tatttactga accgtgcata tcacccagcg
    21601 gttgtgcgga gaacccggcg aaataaccgt tgtccatctt tcgcataccg tcaaaaccta
    21661 acagaatacg accattgaga ttccactgtt cattagtggg atcgctattc acatcagcgt
    21721 tggtcattgc cagcaggcca tgattacttt ccgcatccat attaaattct acgtcgccgt
    21781 agatttttaa atcgccataa cctgaaagca aaagattagg tgaggtggca tttttctttg
    21841 cagattccgc cgttgtctgt ccatttaccg gcgtattgcc ctgggcggcc ttaatttcgc
    21901 ggatctcagc ggcttgttgt gtctgcagct gttttacctg cccttctgct tttgctgccc
    21961 gcatttctgc cgcttccagg cgttcttcca gcaatgccat ccgttgttcc agcgttaaat
    22021 gctgtgtttt tgccataccg gtactacatg ccaataataa accgatggct aaagataatg
    22081 tcgatttttt cattatttac gttccctgaa ataaaggagg ccctacgctt tccaaaagga
    22141 atacgtatat agagaaatat aaagtgtgca ctttcgaatt tttaatccaa cttcagcagg
    22201 ccgatggata gtatcccgcc gcataatatg agtaaatccg ctgatttcct ggaacgtgag
    22261 g
//
LOCUS       DAZKFU020000057        22137 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZKFU020000057 DAZKFU020000000
VERSION     DAZKFU020000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.057"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            186..521
                     /locus_tag="ACHOQQ_RS08660"
                     /old_locus_tag="pgaptmp_001732"
     CDS             186..521
                     /locus_tag="ACHOQQ_RS08660"
                     /old_locus_tag="pgaptmp_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGU4469997.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            979..1422
                     /gene="yjdN"
                     /locus_tag="ACHOQQ_RS08665"
                     /old_locus_tag="pgaptmp_001733"
     CDS             979..1422
                     /gene="yjdN"
                     /locus_tag="ACHOQQ_RS08665"
                     /old_locus_tag="pgaptmp_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGU4469998.1"
                     /translation="MPLSPYISFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1555..2343
                     /gene="phnC"
                     /locus_tag="ACHOQQ_RS08670"
                     /old_locus_tag="pgaptmp_001734"
     CDS             1555..2343
                     /gene="phnC"
                     /locus_tag="ACHOQQ_RS08670"
                     /old_locus_tag="pgaptmp_001734"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGU4469999.1"
                     /translation="MQTIIRVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLIVGDKKPECRVELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2368..3384
                     /gene="phnD"
                     /locus_tag="ACHOQQ_RS08675"
                     /old_locus_tag="pgaptmp_001735"
     CDS             2368..3384
                     /gene="phnD"
                     /locus_tag="ACHOQQ_RS08675"
                     /old_locus_tag="pgaptmp_001735"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4470000.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLSDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISANDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3490..4269
                     /gene="phnE"
                     /locus_tag="ACHOQQ_RS08680"
                     /old_locus_tag="pgaptmp_001736"
     CDS             3490..4269
                     /gene="phnE"
                     /locus_tag="ACHOQQ_RS08680"
                     /old_locus_tag="pgaptmp_001736"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HGU4470001.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4290..5015
                     /gene="phnF"
                     /locus_tag="ACHOQQ_RS08685"
                     /old_locus_tag="pgaptmp_001737"
     CDS             4290..5015
                     /gene="phnF"
                     /locus_tag="ACHOQQ_RS08685"
                     /old_locus_tag="pgaptmp_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGU4470002.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLALWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            5016..5468
                     /gene="phnG"
                     /locus_tag="ACHOQQ_RS08690"
                     /old_locus_tag="pgaptmp_001738"
     CDS             5016..5468
                     /gene="phnG"
                     /locus_tag="ACHOQQ_RS08690"
                     /old_locus_tag="pgaptmp_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGU4470003.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5465..6049
                     /gene="phnH"
                     /locus_tag="ACHOQQ_RS08695"
                     /old_locus_tag="pgaptmp_001739"
     CDS             5465..6049
                     /gene="phnH"
                     /locus_tag="ACHOQQ_RS08695"
                     /old_locus_tag="pgaptmp_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGU4470004.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            6049..7113
                     /gene="phnI"
                     /locus_tag="ACHOQQ_RS08700"
                     /old_locus_tag="pgaptmp_001740"
     CDS             6049..7113
                     /gene="phnI"
                     /locus_tag="ACHOQQ_RS08700"
                     /old_locus_tag="pgaptmp_001740"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HGU4470005.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            7106..7951
                     /gene="phnJ"
                     /locus_tag="ACHOQQ_RS08705"
                     /old_locus_tag="pgaptmp_001741"
     CDS             7106..7951
                     /gene="phnJ"
                     /locus_tag="ACHOQQ_RS08705"
                     /old_locus_tag="pgaptmp_001741"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGU4470006.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7948..8706
                     /gene="phnK"
                     /locus_tag="ACHOQQ_RS08710"
                     /old_locus_tag="pgaptmp_001742"
     CDS             7948..8706
                     /gene="phnK"
                     /locus_tag="ACHOQQ_RS08710"
                     /old_locus_tag="pgaptmp_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGU4470007.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8817..9497
                     /gene="phnL"
                     /locus_tag="ACHOQQ_RS08715"
                     /old_locus_tag="pgaptmp_001743"
     CDS             8817..9497
                     /gene="phnL"
                     /locus_tag="ACHOQQ_RS08715"
                     /old_locus_tag="pgaptmp_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGU4470008.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9494..10630
                     /gene="phnM"
                     /locus_tag="ACHOQQ_RS08720"
                     /old_locus_tag="pgaptmp_001744"
     CDS             9494..10630
                     /gene="phnM"
                     /locus_tag="ACHOQQ_RS08720"
                     /old_locus_tag="pgaptmp_001744"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGU4470009.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10630..11187
                     /gene="phnN"
                     /locus_tag="ACHOQQ_RS08725"
                     /old_locus_tag="pgaptmp_001745"
     CDS             10630..11187
                     /gene="phnN"
                     /locus_tag="ACHOQQ_RS08725"
                     /old_locus_tag="pgaptmp_001745"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGU4470010.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYASALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            11174..11608
                     /gene="phnO"
                     /locus_tag="ACHOQQ_RS08730"
                     /old_locus_tag="pgaptmp_001746"
     CDS             11174..11608
                     /gene="phnO"
                     /locus_tag="ACHOQQ_RS08730"
                     /old_locus_tag="pgaptmp_001746"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HGU4470011.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11610..12368
                     /gene="phnP"
                     /locus_tag="ACHOQQ_RS08735"
                     /old_locus_tag="pgaptmp_001747"
     CDS             11610..12368
                     /gene="phnP"
                     /locus_tag="ACHOQQ_RS08735"
                     /old_locus_tag="pgaptmp_001747"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGU4470012.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNEVIRSPRV
                     ILTHISHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(12445..12774)
                     /gene="yjdP"
                     /locus_tag="ACHOQQ_RS08740"
                     /old_locus_tag="pgaptmp_001748"
     CDS             complement(12445..12774)
                     /gene="yjdP"
                     /locus_tag="ACHOQQ_RS08740"
                     /old_locus_tag="pgaptmp_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGU4470013.1"
                     /translation="MKRHSTLFFFTLLTLTTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12843..15104)
                     /locus_tag="ACHOQQ_RS08745"
                     /old_locus_tag="pgaptmp_001749"
     CDS             complement(12843..15104)
                     /locus_tag="ACHOQQ_RS08745"
                     /old_locus_tag="pgaptmp_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4470014.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDMPLVNTL
                     LNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSGQRV
                     AQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAYGEADVT
                     FPALQRRDELGELARAFNVFARNTESLERTSRLLKEKTTQMEIAQTERQGLEEALLHS
                     QKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLTQRL
                     LAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQLENA
                     IINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGSGMSQAVKSQV
                     FEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAAKNL
                     SLAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAASAE
                     IDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLRKPF
                     TRAQLAQALGQEN"
     gene            15218..16738
                     /locus_tag="ACHOQQ_RS08750"
                     /old_locus_tag="pgaptmp_001750"
     CDS             15218..16738
                     /locus_tag="ACHOQQ_RS08750"
                     /old_locus_tag="pgaptmp_001750"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4470015.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHLLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLAIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16735..17709
                     /locus_tag="ACHOQQ_RS08755"
                     /old_locus_tag="pgaptmp_001751"
     CDS             16735..17709
                     /locus_tag="ACHOQQ_RS08755"
                     /old_locus_tag="pgaptmp_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU4470016.1"
                     /translation="MTTSEKTSAKKILMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            17728..18669
                     /locus_tag="ACHOQQ_RS08760"
                     /old_locus_tag="pgaptmp_001752"
     CDS             17728..18669
                     /locus_tag="ACHOQQ_RS08760"
                     /old_locus_tag="pgaptmp_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4470017.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18724..19407
                     /locus_tag="ACHOQQ_RS08765"
                     /old_locus_tag="pgaptmp_001753"
     CDS             18724..19407
                     /locus_tag="ACHOQQ_RS08765"
                     /old_locus_tag="pgaptmp_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HGU4470018.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGTQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19422..20282
                     /locus_tag="ACHOQQ_RS08770"
                     /old_locus_tag="pgaptmp_001754"
     CDS             19422..20282
                     /locus_tag="ACHOQQ_RS08770"
                     /old_locus_tag="pgaptmp_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HGU4470019.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHAIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFCSAGQA"
     gene            20279..21280
                     /locus_tag="ACHOQQ_RS08775"
                     /old_locus_tag="pgaptmp_001755"
     CDS             20279..21280
                     /locus_tag="ACHOQQ_RS08775"
                     /old_locus_tag="pgaptmp_001755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU4470020.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQWTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21277..21984)
                     /locus_tag="ACHOQQ_RS08780"
                     /old_locus_tag="pgaptmp_001756"
     CDS             complement(21277..21984)
                     /locus_tag="ACHOQQ_RS08780"
                     /old_locus_tag="pgaptmp_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU4470021.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
BASE COUNT         4831 a         6517 c         6272 g         4517 t
ORIGIN      
        1 cttaaataat aaatggaggc aaaaagatta ttttcttttt gcgtttcctt tcaaatgaaa
       61 acgatcgtcg tctaaaatca gcagtacccc cgacaaactc agggattttg tgtataattg
      121 cggccttttt cggcaatctg ccgttttttg gcgcttttgc cctgctgact tttgaggaaa
      181 tccacatgtc attaccacac tgcccaaaat gcaactccga atacacctac gaagataacg
      241 gcatgtacat ctgcccggaa tgtgcctacg aatggaacga cgcagaacct gcacaggaaa
      301 gcgacgagct gatcgttaaa gatgctaacg gcaatctgct ggctgacggc gacagcgtta
      361 ccatcattaa agatctgaag gtgaaaggta gctcttcgat gctgaaaatt ggcaccaaag
      421 taaaaaacat ccgcctggtt gaaggcgatc ataacatcga ttgcaaaatc gacggttttg
      481 gtccgatgaa actgaaatct gagtttgtga aaaagaactg attgtattgt gatcggtaag
      541 ccggataagg cgctcgcgcc gcatccggca acggtgccag atgcctgatg cgacgcttgc
      601 gcgtcttatc aggcctacaa attcccgcac catccgtagg ccggataagg cgtttacgcc
      661 gcatccggca acagtgccag atgcctgatg cgacgcttgc gcgtcttatc aggcctacaa
      721 attcccgcac catccgtagg ccggataagg cgtttacgcc gcatccggca acggtgccga
      781 ctgcctgatg cgacgcttgc gcgtcttatc aggcctacaa attcccgcac cctccgtagg
      841 ccggataagg cgtttacgcc gcatccggca acagtgccga ctgcctgatg cgacgctcgc
      901 gcgtcttatc aggccgcctc tcatctgtat aaatttcgaa ctacacttaa ctggcttctc
      961 ttcactgagg tcaccatcat gccgttaagt ccctacatct cttttgccgg taactgttcc
     1021 gacgcgattg cctattatca acgcacgttg ggcgcggaac tgctctataa aatcagcttc
     1081 ggcgaaatgc caaaatcagc gcaggacagc gccgagaact gcccttccgg aatgcaattt
     1141 cccgataccg ccatcgctca tgccaacgtg cgcattgccg gaagcgacat catgatgagc
     1201 gatgccatgc cgtcaggaaa agccagctac tccggcttta cgctggtgct cgattcgcaa
     1261 caggtcgaag aaggaaaacg ctggtttgat aaccttgccg ctaacggaaa aatcgaaatg
     1321 gcctggcagg aaactttctg ggcgcatggc ttcggcaaag tcaccgataa atttggcgta
     1381 ccgtggatga ttaatgtcgt caaacaacaa ccaacgcaat aatccgccgg gaggcccgcc
     1441 ctcccgcact gtcatcgaat tcccgttaac tcttcatctg ttagtcactt ttaattaacc
     1501 aaatcgtcac aataatccgc cacgatggag ccactttttt agggaggctg catcatgcaa
     1561 acgattatcc gcgtcgaaca actgagcaaa accttcaacc agcatcaggc gctgcacgcg
     1621 gttgatctga acattcatca cggtgaaatg gtggctctgc ttgggccgtc cggttccggc
     1681 aaatccaccc ttttacgtca cttaagcggc ctgattgtcg gcgataaaaa gccagaatgc
     1741 cgcgtcgaac tgctgggccg cactgtccag cgcgaaggcc gcctggcccg tgatatccgc
     1801 aaaagccgcg ccaacaccgg ctacatcttc caacaattca acctggtgaa tcgcctgagc
     1861 gtactggaga acgtgctgat tggcgcgctc ggcagcacgc cgttctggcg cacctgtttt
     1921 agctggttca ccggcgagca gaaacagcgc gcgttacagg cgctgacccg cgttggcatg
     1981 gtgcattttg cccatcagcg cgtttccacc ctctccggtg gccagcagca gcgtgtggcg
     2041 attgcccgcg cgctgatgca gcaggcgaag gtgattctgg ccgatgaacc catcgcctcg
     2101 ctggacccgg aatcggcgcg catcgtgatg gacaccctgc gcgacatcaa ccagaacgac
     2161 ggtatcaccg tggtcgtcac gctgcatcag gtggattacg ccctgcgcta ctgcgaacgc
     2221 atcgtcgccc tgcgtcaggg acacgtcttc tacgacggca gcagccaaca gtttgataac
     2281 gaacgttttg accatctcta ccgcagcatt aaccgcgtcg aagagaacgc gaaagctgcc
     2341 tgacatcccc atcattgagg aaaacgaatg aacgctaaga taattgcctc gctggccttc
     2401 accagcatgt tcagcctcag caccctgtta accccggcgc atgccgaaga gcaggaaaag
     2461 gcgctgaatt tcggcattat ttcaacggaa tcacagcaaa acctgaaacc gcaatggacg
     2521 ccgtttttac aggatatgga gaagaagctg ggcgtgaagg tcaacgcctt ctttgccccg
     2581 gactatgcgg gcattatcca gggaatgcgt ttcaacaaag tggatatcgc ctggtacggc
     2641 aacctgtcgg cgatggaagc agtggatcgc gccaacggcc aggtcttcgc tcagaccgtc
     2701 gcggcggatg gatcgccggg ttactggagc gtgttgatcg tcaacaaaga cagcccgatc
     2761 aacaacctga gcgatctgct ggcgaagcgg aaagatctca ccttcggcaa tggcgatcct
     2821 aactccacct ctggcttcct cgtccccggt tactacgtct ttgccaaaaa caatatctcc
     2881 gctaacgact ttaagcgcac cgtcaacgcc gggcatgaaa ccaacgcgct ggccgtcgcc
     2941 aacaagcagg tggatgttgc cactaacaac accgaaaacc tcgacaagct gaaaacctcc
     3001 gcgccggaga agctcaaaga gctgaaagtg atctggaagt cgccgctgat cccaggcgat
     3061 ccgatcgtct ggcgcaagaa tctgtccgaa accaccaaag acaagatcta cgacttcttt
     3121 atgaattacg gcaagacgcc ggaagagaaa gcggtgctgg aacgcctggg ctgggcgccg
     3181 ttccgcgcct ccagcgacct gcaactggtg ccgattcgcc agctcgcgct gtttaaagag
     3241 atgcagggcg tgaaaagcaa taaaggactg aatgagcagg acaagctggc aaaaaccacc
     3301 gagattcagg cgcagctgga tgacctggac cgcctgaaca acgcgttaag cgcgatgagt
     3361 tcggtgagta aagcggtgca gtaaatcgta ggtcggataa gacgccccgg cgtcgcatcc
     3421 gacaatgtgc aggcgttgat gccggatgcg gtgcaagcac cttatccggc ctacagaccg
     3481 gagccaaaca tgcaaaccat caccatcgcc ccacccaagc gtagctggtt ctcgcttctg
     3541 agctgggccg ttgttctcgc cgtgctggtc gtctcgtggc agggcgcgga aatggccccg
     3601 cttacgctta tcaaagacgg cggcaacatg gcaaccttcg ccgccgactt cttcccgccc
     3661 gatttcagcc agtggcagga ttacctcacc gaaatggccg tcacgctgca aatcgccgta
     3721 tggggcaccg cgctggcagt ggttctgtcc atcccgttcg gtctgatgag cgccgaaaac
     3781 ctggtgccgt ggtgggttta ccagcccgtg cgccgcctga tggacgcctg ccgcgccatt
     3841 aacgaaatgg tcttcgccat gctgttcgtg gtcgccgtcg gtctcggacc gttcgctggc
     3901 gtgctggcgc tatttattca caccaccggc gtgctctcca agctgctttc cgaagcggtg
     3961 gaagcgattg aacctggccc ggtggaaggc attcgcgcca ccggtgccaa caaactcgaa
     4021 gagatcctct acggcgtgct gccacaggtg atgccgctgc tgatctccta ctccctctat
     4081 cgcttcgaat ccaacgtccg ctcggcgacc gtcgtcggca tggtcggcgc gggcgggatc
     4141 ggcgtcacgc tgtgggaagc gattcgcggt ttccagttcc agcaaacctg cgccctgatg
     4201 gtgctgatta tcgtcacggt cagcctgctg gatttcctct ctcaacggtt gcgtaagcac
     4261 tttatctgat tagcgaggca ttgatagcta tgcacttgtc tacacatccg accagctacc
     4321 cgacacgcta tcaggagata gccgcaaaac ttgagcagga gcttcgtcaa cactaccgct
     4381 gcggcgacta tcttcccgcc gagcagcaac tggcagcgcg ctttgaggtg aaccgccaca
     4441 ccctgcgccg cgccatcgac caactggtgg aaaaaggctg ggtacagcgc cgtcagggcg
     4501 tcggcgtact ggtgctgatg cgcccgttcg attacccgct caacgcccag gcgcgtttta
     4561 gccagaacct gctggatcag gggagccatc ccaccagcga aaaactgctg tctgtgctgc
     4621 gcccggcctc cggtcacgtt gcagacgcac tggggattac cgagggcgag aacgttatcc
     4681 acctgcgcac cctgcgccgg gtcaacggcg ttgcgctctg tttaatcgac cactacttcg
     4741 cggacctcgc cctctggccg acgctgcaac gcttcgacag cggctcgctg cacgattttc
     4801 tgcgcgagca aacggggatt gcgctgcgcc gcagccagac gcggatcagc gcccgccgcg
     4861 cccaggccaa agagtgccag cgtcttgaaa tcccgaatat gtcgccgctg ctgtgcgtgc
     4921 gcacccttaa ccaccgtgac ggtgaaagca gcccggcgga gtactccgtc agcctgacgc
     4981 gcgccgacat gattgaattt actatggagc actgaatgca cgcagatacc gcgacccgcc
     5041 agcactggat gtccgtactg gcgcacagcc aaccggcaga actggcagca cgcctgaacg
     5101 cgctaaacat caccgccgac tatgaggtga tccgcgccgc tgaaactggc ctggtacaga
     5161 ttcaggcgcg gatgggcggc accggcgaac gcttttttgc cggcgacgcc acgctgaccc
     5221 gcgccgccgt acgtctgact gacggcaccc tcggctacag ctgggtgctg gggcgcgaca
     5281 aacagcacgc cgaacgctgc gcgctgattg acgcgctgat gcagcaaacc cgccattttc
     5341 aaaacttatc agaaacgctt attgccccgc tggacgctga ccgtatggca cgcattgccg
     5401 cacgccaggc cgaagtgaac gccagccggg tcgacttctt tacgatggtt cgcggagaca
     5461 acgcatgacc ctggaaaccg cttttatgct tcccgtgcag gatgcccagc acagttttcg
     5521 tcgcctgtta aaggccatga gcgagccggg cgtgattgtc gccctgcatc agctcaaacg
     5581 cggctggcag ccgctgaata tcgccaccac cagcgtgctg ctgacgctgg ccgataacga
     5641 cacgccggtg tggcttgccg cgccattgag caacgatatc gtcagccaga gcctgcgttt
     5701 tcataccaac gcgccgctgg tcagccagcc ggaacaggcg accttcgcgg taacggatga
     5761 ggcgatttcc agcgaacagc ttaacgccct ttccaccggc accgccgttg cgccggaagc
     5821 gggcgcaacg ctgattttac aggtcgccag cctgagcggc gggcgcatgc tgcgtctcac
     5881 cggcgcgggt attgccgaag aacgaatgat cgccccgcag ctgccggagt gcattctgca
     5941 cgaactcacc gagcgcccgc acccgttccc gctcggcatc gacctgatcc tgacctgcgg
     6001 cgagcgcctg ctggctgttc cgcgaaccac tcatgtggag gtgtgctgat gtacgttgcc
     6061 gtgaaagggg gcgagaaggc gatcgacgcc gcccacgccc tgcaagagag ccgacgccgg
     6121 ggcgataccg atttgcctga actgagcgtc gcccagattg aacagcagct taacctcgca
     6181 gtagatcgcg tgatgaccga aggcggcatt gccgaccgcg aactggcggc gctggcgctg
     6241 aaacaggcca gcggcgataa cgtcgaagca attttcctgc tgcgcgccta ccgcaccacg
     6301 ctggcgaagc ttgcggtaag cgagccgctc gacaccacgg agatgcgtct cgaacggcgt
     6361 atttcagcgg tttataaaga cattcccggc ggccagctgc ttggcccaac ctacgactac
     6421 acccatcgcc tgctcgattt caccctgctg gcaaacggcg aagcgccgac gctgaccacc
     6481 gccgacagcg aacaacagcc gtcgccgcac gttttcagcc tgctggcgcg tcaggggctg
     6541 gcgaagtttg aagaggatag cggcgcacag ccggatgaca tcacccgcac gccaccggtt
     6601 tacccctgct cacgttcttc ccgcctgcaa cagctcatgc gcggcgacga aggctatttg
     6661 ctggcgctgg cctactccac ccaacgcggt tacgggcgca atcacccgtt cgcgggtgaa
     6721 atccgcagtg gttacatcga cgtgtcgatt gtgccggaag agctgggatt tgcggtgaac
     6781 gtcggcgaac tgctgatgac tgagtgtgaa atggtcaacg gttttatcga cccaccggat
     6841 gaatctccgc acttcacgcg cggctacggg ctggtgttcg gcatgagcga gcgcaaagcg
     6901 atggcgatgg cgctggtcga ccgcgctctg caagccccgg agtacggcga gcacgcgaca
     6961 ggcccggcgc aggatgaaga gttcgtgctg gcacatgccg acaacgtcga agccgcaggc
     7021 tttgtctcgc acctcaaact cccgcactac gtcgatttcc aggccgaact ggagctactc
     7081 aaacgtctgc aacaggagca gaaccatggc taatctgagc ggctacaact ttgcctacct
     7141 cgacgagcag accaaacgca tgatccgccg cgccatctta aaagcggtgg caatccccgg
     7201 ttatcaggtg ccgtttggcg ggcgcgagat gccaatgccg tacggctggg gaaccggcgg
     7261 cattcagctt accgccagcg tgattggcga aagcgacgtg ctgaaggtga ttgaccaggg
     7321 cgcggatgac accaccaacg ccgtgtcgat tcgcaacttc tttaagcgcg tgaccggggt
     7381 aaacaccacc gagcgcacgg acgatgcaac ggtcatccag acgcgtcacc gcatccccga
     7441 aacgccgctg accgaagatc agatcattat tttccaggtg ccgatccccg aaccgctgcg
     7501 ctttatcgag ccgcgcgaaa cggaaacccg caccatgcac gcgctggagg agtacggcgt
     7561 gatgcaggtg aagctgtatg aagatatcgc ccgcttcggt catatcgcca ccacctacgc
     7621 ctatccggtg aaggtgaacg ggcgctacgt aatggacccg tcgccgatcc cgaaattcga
     7681 taacccgaaa atggacatga tgcccgccct gcaactgttc ggcgccgggc gtgagaagcg
     7741 catctatgcg gtgccgccgt ttacccgcgt ggaaagcctc gatttcgacg atcacccgtt
     7801 caccgttcag cagtgggatg agccatgcgc catctgcgga tcgacccaca gctatcttga
     7861 tgaagtggtg ctggatgacg ccggaaaccg catgtttgtc tgctccgata ccgattattg
     7921 ccgccaacag agcgaggcaa aaagccaatg aatcaaccgt tactttcggt caataacctg
     7981 acccaccttt acgcgccggg caaaggcttt agcgatgtct cttttgattt atggccgggg
     8041 gaagtgctgg gcattgtcgg ggaatccggc tccgggaaga ccacgctgct gaaatcaatt
     8101 tccgcgcgtc tgacgccgca gcagggggag attcactatg aaaaccgttc cctctatggc
     8161 atgagcgagg ccgaccgccg tcgcctgctg cgcaccgaat ggggcgtggt gcatcagcat
     8221 ccgctcgacg gcctgcgccg ccaggtgtcg gcaggcggca atatcggcga gcggctgatg
     8281 gcgaccgggg cacgtcatta cggcgatatt cgcgccaccg cgcagaagtg gctggaagag
     8341 gtggaaattc ccgccaaccg gatcgacgac ctgccgacca ccttttccgg cggcatgcag
     8401 cagcgtttgc agattgcccg caacctggtg acgcatccga agctggtatt tatggacgag
     8461 ccaaccggcg ggctggatgt gtcggtgcag gcgcgcctgc tcgacctgct gcgcggcctg
     8521 gtggtggagc tgaacctcgc ggtggtgatt gtcacccacg atttaggcgt cgcccgcctg
     8581 ctggcggacc gtttgctggt gatgaagcag gggcaagtgg tggagagtgg gttaaccgac
     8641 cgcgtgctcg acgacccgca tcatccgtat acacagctgc tggtgtcatc ggttttgcag
     8701 aattgagccg gtgccggatg cggcgtaaac gccttatccg gcctacaaat gcgctccccg
     8761 taggtcggat aagacgcgtc agcgtcgcat ccgacacccg aaccacgagg cgaaaaatga
     8821 ttaacgtaca aaacgtcagt aaaaccttca tcctgcatca gcaaaacggc gtgcgcctgc
     8881 ccgtcttaca acgcgcctca ctcaccgtca acgcgggcga atgcgtggtg ctccacggcc
     8941 actccggcag cggcaaatca accctgctgc gctcgctgta cgccaactat ctgcccgacg
     9001 aagggcaaat ccagatcaaa cacggcgacg aatgggtcga tctggtcacc gcgccagcgc
     9061 gcaaagtggt ggagatccgc aaaaccaccg tcggctgggt gagccagttt ctgcgcgtca
     9121 tcccgcgtat ttcagcactg gaagtggtga tgcagccgct gctcgatacc ggcgtaccgc
     9181 gtgaagcctg cgccgctaaa gccgcgcgtc tgctcacccg cctgaacgtg ccggaacgcc
     9241 tgtggcacct ggcgccatcg accttctccg gcggcgaaca gcagcgcgtc aacatcgccc
     9301 gcggctttat cgtcgactac cccattctgc tgctcgacga acctaccgcc tcgctggacg
     9361 ctaaaaacag cgccgccgta gtggaactga tccgcgaagc caaagcccgt ggcgcagcca
     9421 tcgtcggcat tttccatgac gaagccgtac gcaatgatgt cgccgaccgc ctgcacccaa
     9481 tgggagcctc ttcatgatta tcaataacgt taaactggtg ctggaaaacg aagtggtgca
     9541 cggttcgctg gagatgcagg atggtgaaat ccgcgccttt gccgaaagcc agagccgcct
     9601 gccggaggcg atggacggtg aaggcggctg gctgctgccg ggattgattg agctgcatac
     9661 cgataacctc gataaattct tcaccccgcg cccgaaagtc gactggcccg cccattctgc
     9721 gatgagcagc cacgacgcat taatggtggc aagcggcatc accaccgtac tggatgcggt
     9781 ggcgattggc gacgtgcgcg acggcggcga tcggctggag aatctggaga agatgatcaa
     9841 cgccatcgaa gagacgcaga aacgcggcgt caaccgcgcc gagcaccgcc tgcacctgcg
     9901 ctgcgagctg ccgcatcaca ccacgctgcc gctgtttgaa aaactggtgc agcgcgagcc
     9961 ggtgacgctg gtgtcgctga tggaccactc gccgggccag cgccagttcg ccaaccgcga
    10021 gaagtaccgt gaatattatc agggcaaata ctcgctcact gatgcgcaga tgcagcagta
    10081 cgaagaagag caactggcgc tcgccgcacg ctggtcgcag ccgaatcgcg aatccatcgc
    10141 cgccctgtgc cgcgcgcgaa aaattgcgct tgccagccac gatgacgcca cccacgccca
    10201 cgttgccgaa tcccatcagc ttggcagcgt gatcgccgaa tttcccacca cgttcgaagc
    10261 ggcagaagcc tcgcgcaagc atggtatgaa cgtgctgatg ggcgcgccaa atatcgtgcg
    10321 tggcggttcg cactccggca acgtggcggc cagtgaactg gcgcagcttg gcctgctgga
    10381 tatcctctct tccgactact accccgccag cctgctggat gcggcgttcc gcgtggccga
    10441 tgaccagagc aaccgcttta cgctgccgca ggcggtgaag ctggtgacta aaaatccggc
    10501 gcaggcgctt aatctccagg atcgcggggt gattggcgag ggcaaacgcg ccgacctggt
    10561 gctggcgcat cgcaagggca accacattca tatcgaccac gtctggcgtc agggtaaaag
    10621 ggtgttctga tgacgggaaa actgatttgg ttaatggggc cgtccggctc cgggaaagac
    10681 agcctgctgg cggaactccg cctacgggaa caaactcagt tactggtggc gcatcgctac
    10741 atcacgcgcg acgccagcgc tggaagcgaa aaccatatcg ccctgagcga gcaggagttt
    10801 tttacccgcg cggggcaaaa cctactggcc ttaagctggc acgccaacgg cctgtattat
    10861 ggcgtcggcg tcgagataga cctctggctg cacgccggat tcgacgtgct ggtcaacggc
    10921 tcacgcgccc atctgccgca ggcgcgggca cgctatgcat cggcactgct gcccatctgc
    10981 ttacaggtgt cgccagagat cctgcgccag cgcctggaaa accgtggtcg tgaaaacgcc
    11041 agtgaaatta acgcccggct ggcgcgcgcc gcccgctata ctcctcagga ttgccatacg
    11101 ctcaacaatg acggcagcct gcgccagtcg gtcgacacgc tgctgacgct gattcatcag
    11161 aaggagaaac accatgcctg cttgtgagct tcgcccggcc acgcagtacg acaccgacgc
    11221 ggtttacgcg ctgatttgtg agctaaaaca ggcggagttt gaccatcacg cgtttcgcgt
    11281 gggctttaac gctaacctgc gtgacccaaa catgcgctac catctggcgc tgctggatgg
    11341 cgaagttgtc ggtatgatcg gcctgcattt gcagtttcat ctgcatcatg tcaattggat
    11401 cggcgaaatt caggaactgg tggtgatgcc gcaggcgcgc ggtctgaacg ttggtagcaa
    11461 gttgctggcg tgggcagaag aagaagcccg ccaggccggg gccgaaatga ccgagctttc
    11521 gaccaacgtg aagcgccacg acgcacaccg tttctatctg cgcgaaggct acgagcagag
    11581 ccacttccgc ttcacgaagg cgctgtaaca tgagcctgac cctcacgctc accggcaccg
    11641 gcggcgcaca gggcgttccg gcatggggct gcgagtgtgc ggcctgcgcc agagcgcggc
    11701 gctcaccgca ctatcgccgc cagccatgca gcggcgtagt gaagtttaac gaggcgatca
    11761 ccctgatcga cgccgggcgg cacgatctcg ccgatcgctg gtcgccagga tcgttccagc
    11821 agtttttgct gacgcactac catatggatc acgtccaggg gctgtttccg ctgcgctggg
    11881 gcgtgggcga tacgatcccg gtttacggcc cgccggatga ccagggttgc gacgatctgt
    11941 ttaaacatcc gggcctgctt gatttcagcc acacagtgga accgtttgtg gtgtttgatt
    12001 tgcaggggtt gcaggtcacg cccctgccgc tcaaccactc aaaactgacc ttcggttatc
    12061 tgctggaaac ggcgcacagc cgggtggcgt ggctgtctga caccgctggc ttgccggaaa
    12121 aaacgctgaa atttttacgc aataatcagc cgcaggtaat ggtgattgat tgcagccacc
    12181 cgccgcgcga ggatgcgcca cgtaatcact gtgatttaaa caccgtgctg gcgctgaatg
    12241 aagttatccg ctcgccgcgg gtgattctga cacacatcag ccaccagttt gatgcgtggc
    12301 tgatggagaa cgcgctgccg ccggggtttg aagccgggtt tgatgggatg gagattgggg
    12361 tggcgtgatg agagggaatg tgcgcgctgg ccccctcacc ctaaccctct ccccagaggg
    12421 gcgatgcctg ctccataccc aacatcatcg cccatactca tcttccattc tccgctcttc
    12481 atcctccagc tgccgacgct cctgatcaag ctggcgctgg cgatcgtcca gctgcctgcg
    12541 gcgatcttca aactggcggc ggcggtcgtc atattgtctg cgccgatcgt cgctcacttc
    12601 acgctgccag ccatcgtcgc gcgaatcttc ataatctcgc ccacggtcag ggttataagc
    12661 gtcgttaatc gcctgctgaa tattgccaat ggtgtcgtcg ataatatcgg cctgggccgg
    12721 tacggtggtc agcgtgagca gggtgaaaaa aaagagcgtg gagtggcgtt tcataaactc
    12781 ggcctcgcaa caaagttaat acaaggtagg caaaggtgcc tgttacgagt agaggaggat
    12841 tctcaattct cttgccccag cgcctgcgcc aactgcgcac gggtaaaggg tttgcgcaac
    12901 agcgccacgt ctggcagcgc ggggttatgg ctggggcgta aatcctgacc gctgatgagt
    12961 agcagcgtca ggtgcgggta aagtttgcgg gccgcattga ccacttctgc gccgctcatg
    13021 ccgccgggga gcattaaatc gctgataaaa atatcgattt ctgctgatgc cgccagcaga
    13081 tccagcgcct gttcgccgct gcttgcttcc agcgtcaaat agcccagcag gtgcaactgt
    13141 tcgcagatgg tctggcgcac cgctgcttca tcttccagca ctaacaccag tttatcgccg
    13201 ctgacagaag cctgttctac tgccgctagc gataggtttt tcgccgccgt cagcgcgcgg
    13261 ggaagctgta atctgaccgt tgtcccctgc cccggcgcgc tttcaatttc aacgcgccca
    13321 ccggactggc gcacaaaacc atagaccatc gacaaaccca gcccgctgcc gctgcccgtc
    13381 tgtttggtgg taaagaatgg ctcaaatacc tggcttttca ccgcctgcga cataccgctg
    13441 ccatgatcga tgacctccag tgccaccatg tcctgacgct gcccactgct gcgagtaacg
    13501 cgctgattcc aggtacgaat tttgatgatt cccgtctgcc cttccatcgc gtcacgggcg
    13561 ttcatcacca ggttgatgat ggcattttcc agttggctga tgtcaatcca cgcgggccac
    13621 gccggagact gcgcttcaat ctccagcgcc agcgttgccg gtaaggagtg gcgcattaat
    13681 tcgcttaact cttccagcaa tgttttcagt tcaacggctt gcggatgtaa ggactgcttg
    13741 cgggaaaacg ccagcagtcg ttgtgtcagt agcgccccac gttcggcagc ttgcagcgcc
    13801 cgctgtattc gcggcgcgtc tggcgagtcc ggattcacca gctccaggct gccaataatc
    13861 accgccagca gattattaaa atcatgggcc agcccgcccg tcagttgccc gaccgctttc
    13921 agtttctggc tgtgtaatag cgcctcttcc aacccctgcc gctcagtttg ggcaatctcc
    13981 atctgcgtgg tcttctcttt tagcagccgt gaggttcgtt cgagtgactc ggtattgcgg
    14041 gcaaaaacgt taaaggcgcg cgccagttca cccaactcat cacgccgctg taacgccgga
    14101 aaagtgacgt ccgcttcgcc gtacgccagg cggctcatgg cgcgggaaat cgccgtcagg
    14161 ttagtgccga ggttgcgata gatataccag ccggcaaacg cggtgatcac caccgctaac
    14221 agggcgaaaa tcatgatcac cctctcgccg gagcgcaaat cctggtaact ttgcgccacg
    14281 cgctgaccgg agagcgtcgc cacctgttcg gcgtaacggt tgatgtcgcc attgaggatc
    14341 gccaccagcg ctttgatgtg aaacatatac cagctgattg ccagatcgct ttgctccaga
    14401 gcggcagaaa gcggcgcgag tttacgcatt tcatcattga aatcattgag taacgtatta
    14461 accaatggca tatcgctgtg ggtcggcaac gcagacatta ccccgaccag ttgatgaata
    14521 atcgcctgcg gcgcgggggt ggcaatagcg gcaacaatca gtcggttcat ctcgttaagc
    14581 aaaatatcat cttgcgcgtg agtgactttt tgcagatgcc gcagataact taagttttga
    14641 tacagcgaac ttaacagcgc gttgcgctcc agatgccgcc tttgcccgcg ctccagcata
    14701 ccgccgacgc tggtttgcag ttcgttgcta cgcgtgatga ttcgcgtcac cagcccggct
    14761 tcctgctgcg ctaaaggcgc gttcgccagt tgtgcaagcg agctttttaa tgcccgctgt
    14821 gtctcctgca accgctcggc ttcgccttta tattccagcg ctcccaccac ctgcgacaat
    14881 cgcaccgccg ccgttgccac gtttgccgtg tcgcgcgcca gattcaggct gccggtcata
    14941 tcatccaccg tttgctgctg caccagctcc tgcatctggc tggcatgacg aaagcccatt
    15001 accgccacgc cgctgaccat caacgtcacc gccaccacca gcaaattcaa aagcagcaat
    15061 cgcccgcgcg cgctggcaaa aaaatgacgt ccggggtgcg ccatatcacg ctccgtgaag
    15121 aaaaatcgca atatgacaaa tatgaacggc ttctgacatt ttgcatttac tttcctgtga
    15181 tggagtgctg atatcgacaa cctcgcagga gtccaccatg aacagcacgc cagttctgga
    15241 gatgcgcaat attgccaaag ccttcggcaa attttatgcg ctcaaagggg tggatttgac
    15301 ggtctaccct ggcgagatcc atgccctgat gggggaaaac ggcgcgggaa aaagcacgct
    15361 gatgaaggtg ctggcggggg cgtataccgc caccagcggc gagatcctca tagacggcaa
    15421 gccctttcac attcgcacgc caaaagatgc cttaagcgcc ggtattacgc tgatttatca
    15481 ggagatgcag ctggcaccga atctgtcggt ggcagaaaat atttttctcg gcagcgagct
    15541 ttcccacggc gggctggtgc agcgtaaaga gatgctagtg caggcgcaaa aagtgatcga
    15601 ccgcctcggc gcgcagttta acgccagcga taaggtcatg acgctgacca ttgccgagca
    15661 acagcaggtc gaaatcgccc gcgcactaca tcgcaacagc cgcattctgg tgatggacga
    15721 acccaccgct gccctctcct cccgtgaaac tcaccgtctg tttgaactga ttatgcggct
    15781 gcgcgatgaa gggatggcga ttatctacat cagccaccgt atggcggaag tgtatgagct
    15841 ttccgatcgc gtcagcgtgc tgcgcgacgg gcaatacgtt ggcagcctga cccgcgataa
    15901 cctcaatgcc ggggagctgg tgcggatgat ggtcggcagg ccactgagcg atctgttcaa
    15961 taaagagcgc gatatcccgc tcggtaaagc ccgcctgaat gttcacctcc tgaccgacgg
    16021 cggcaaagtc cagccgagta gcctgctggt gcgttccggc gaaattgttg gcctcgccgg
    16081 actggtgggt gccggacgtt ccgaactggc gcagttgatc ttcggcgtgc ggaaagcgac
    16141 aggcggaatg attgaagtcg atggtgaacc ggtggtgatc cactccccgc gcgaagccat
    16201 cgatcttgcc attggttttc tcaccgaaaa ccgcaaagaa caaggcttat tccttgaaat
    16261 ggcagcagcc gaaaacatca ccatggcaac cctggagcgc gatgcccgct ggggaatgct
    16321 caatcgcaaa aaagcgcaaa ccatttccga tgacgccatt aagttgctca acattcgcgt
    16381 gcctcatgcc caggtacgcg cgggcgggct ttccggcggc aatcagcaaa aactgttgat
    16441 ctcccgctgg gtggcgattg gcccgcgcat tttactgctc gatgaaccca cccgcggcgt
    16501 ggacgttggc gccaaaagtg agatctaccg gatcatgaac gagatggcgc gcaagggcgt
    16561 ggcgatcctg atgatctcca gcgaactgcc ggagatagtg ggaatgagcg atcgcgtcta
    16621 tgtgatgcgc gaaggcagca ttgccggtga gttaaacggc aaaaacatca cccaggaaaa
    16681 cattatgacc ttagcgactg gcgtcaacga cgcccattcc caggcggtaa cctcatgacg
    16741 acctctgaaa aaacctctgc caaaaaaata ctgatgagcg atctgatgca aacggtcggc
    16801 atcttgccca ttttgattct gatcgtggcg gtatttggct ttatcgcgcc aaactttttt
    16861 accgaaagca acctgctgaa tattacccgc caggcatcga tcaacattgt tctggcggcg
    16921 gggatgacct tcatcatttt gaccggcggg atcgatcttt ccgtgggttc gatcctcggc
    16981 accacagcgg tggcggcgat ggtcgtctcc cttagccctg agttcgccat gctgtcgatc
    17041 ccggcagcgc tgatgctcgg cctgctgctg gggctgttca acggcgcgct ggtggccttt
    17101 gccggattgc caccgtttat cgttacgctt ggcacctata cggcgctgcg cggggcggcg
    17161 tatctgctgg cggacggcac cacggttatc aactccaaca ttaatttcga gtggataggc
    17221 aacaactatc tcgggccggt cccatggctg gtggtgattg cgctggcggt gatcgtggtg
    17281 tgctggttta ttctgcgccg caccacgctt ggcgttcata tctacgccgt cggcggcaat
    17341 atgcaggcgg cgcgtcttac cggcattaag gtctggctgg tgctgctgtt tgtttatggt
    17401 atgagcggcc tgctttcagg gctgggcgga attatgagcg cctcaaggct gtacagcgcc
    17461 aacggcaact taggtatggg gtacgagctg gacgccattg cggcggtgat cctcggcggc
    17521 accagttttg tcggcggcat cggcaccatc accggcacgc tggtcggcgc gctaattatc
    17581 gccacgctaa acaacggcat gaccttaatg ggcgtctctt acttctggca actggtgatc
    17641 aaaggggcgg tgatcatcat tgcggtgctg atcgacaaat accgtacccg acaccatcaa
    17701 agtgcataaa cacatgagga atacagcatg agattgaagc ccttagtgac cgcgctctgt
    17761 gcaggcgcgc tgctggccgc agcaccgttc gcccaagcga aagaactgaa atcgataggc
    17821 gtaacggtgg gcgatctggc gaacccgttc ttcgtgcaga tcaccaaagg cgcggagctg
    17881 gaagcacgca aactggcagg cgataacgtc aaagtgacgc tggtttccag cggctacgat
    17941 ctgggtcagc aggtgtcgca gatagacaac tttatcgccg ctaacgtcga tatgattatc
    18001 ctcaacgctg ccgattcaaa agggatcggc ccggcggtga aacgggcgaa agatgccggg
    18061 atcgtagttg tcgcggttga cgtagcggca gaaggtgctg atgccaccat cacctccgat
    18121 aacacccagg cgggcgaaat ggcctgtaaa tacatcaccg atcgcctgaa aggcaaaggc
    18181 aatgtggtga tcatcaacgg cccgccggta tccgccgtgc aaaaccgcgt cgaaggatgc
    18241 cagactgaat ttaaacgcca tcctgacatc aaagtgctct ctgacaacca gaacgcgaaa
    18301 ggcagtcgtg aaggcgggct ggaggtaatg acgggactgc tggcggcaaa tccgaagatc
    18361 gacggcgtat ttgcgattaa cgatcccacc gcaatcggtg ccgatctggc agcaaaacag
    18421 gcgcaacgta acgagttctt tattgtcggt gtggacggaa gcccggacgc cgaagaggcg
    18481 ctcaagcgtg agaattcgtt gtttgtcgct actccggcgc aagatcccca ggtgatggca
    18541 gcgaaagcgg tggaaattgg ctacgacatt ttgcagggca aacctgcgcc gaaagagccg
    18601 gtgctgatcc cggtgacgat gatcgataaa aacaacgtcg gcaattacaa aggctggacg
    18661 gtgaaataag tccacacgcc gggcggcgca gctgcccggt caccgctaag gagaacgctt
    18721 tcgatgaaac gctccgctat aaacgacatt ctcggccata cacggcaatt cttttcgcaa
    18781 catgacgtac atctgccgcc ctttgccagt ttctcgcctg ctcaatggca gcagctcgac
    18841 accgccgcgt gggaggaggt ttttgatctt aaactcggct gggatgttac cgcttttggt
    18901 cgcaacaact ttgcagcaca cgggctgacg ctgtttaccc tacgtaacgg ctcagcaaaa
    18961 ggaatgccgt atgtgaaatg ttacgccgag aagatcatgc atgtgcgcga tgcgcaggtc
    19021 acgccaatgc attttcactg gcgtaagcgt gaggacatta tcaatcgcgg cggcggtaat
    19081 ttaattgttg aactgtggaa tgccgatagt aacgagcaaa cggctgatag tgacattacg
    19141 gtggtgatag acggctgccg ccagaaacat actgcaggca ctcagttgcg cctgtctccc
    19201 ggcgaaagta tctgcctgcc gcccggcctg tatcacagct tttgggcgga aaccggcttt
    19261 ggcgatgttc tggttggcga agtctcttct gtcaatgacg acgaccacga taaccacttt
    19321 ttacagccac tggaccgcta caacctgatt gacgaagacg aacctgcaca gttggtattg
    19381 tgtaacgaat accgccagtt ccgctgaata aggataacgt tatgccattg atttctctcg
    19441 ctgaagggct ggcacatgcc cgcgaacatc actattcgtt aggtgcgttt aatgtgctgg
    19501 actcgcactt tttacgcgcc ttgtttgctg ctgccagaca agaacgttcg ccctttatta
    19561 tcaacattgc cgaagtacat tttaaatata tctcgctgga ttccctggtt gagacagtga
    19621 aatttgaagc ggcgcgtcat gccattccgg tggtgttaaa tctcgatcac ggactgcatt
    19681 ttgaatcggt ggtgcaagcg ttgcggctgg gattcagctc ggtaatgttt gatggctcca
    19741 cgctggggta tgaagaaaat gtccgccaga cgcgggaagt ggtgaaaatg tgccatgccg
    19801 ttggcgtatc ggtagaagct gaactgggcg cggtgggtgg cgacgaaggc ggtgcgctct
    19861 atggtcatgc cgatgaatcc ctgtttaccg atccgcaact ggcgcgtgat tttgtcgacc
    19921 gtaccggcat tgacgcgttg gccgtggcga ttggcaatgc acacggcaaa tatcgcggcg
    19981 aaccgaaact cgatttcccg cgcctcgacg ccatccgcca gcaagcggct atcccgctgg
    20041 tgttacacgg cggctcaggg attagcgatg ccgatttccg ccgcgccatc gaactgggta
    20101 tccacaaaat caatttctac actggcatgt cgcaagcagc gctggctgcc gttgaggccc
    20161 gaatggcgca ccgtcagccg atgtatgacg agtttgccga gttactgctg gcagtggaag
    20221 ccgccatcag cgatacggtt gcacaacaga tgcgtatttt ctgtagtgcg gggcaggcat
    20281 gatggagcgt aaagggatta tcgccgcagg caatatgctg gttgaccacg tccatcagat
    20341 tatccagtgg ccagagcgcg gctggctggc ggagatcacc cacagcgaac gctccaccgg
    20401 cggtgcgccg cttaacgtcc tgctgacgct ggcgaaaatg cgcaccgggc tgccgttaca
    20461 ggcggttggg ctaatcggtc aggacagcga cggcgattac atcatggcga tgcttgagca
    20521 atatcacgtc aatcgtcagc atgtgcagcg cactaccttt gccccgacgt cgatgtcgca
    20581 ggtgatgaca gacccaagcg ggcagtggac gtttttccat tcgccgggtg caaacagact
    20641 tttggatctt ccggcgttcg atcagctcga taacaccatg aagattttcc atctcggcta
    20701 tttactgttg ctcgacagcc tggatatgcc ggacgatgtt tacggcaccc gtagcgcacg
    20761 cctgctggcg caaatgcgtg atactggctt tgaaacctca ctcgacctgg tctcccgtaa
    20821 gggcgatccg cgttatcagc cgctggtgct gcccgcgttg cgccatctgg attatctggt
    20881 aattaacgaa ctggaggccg gggagtttag cgggctggaa attcggttac ccaacggcga
    20941 accgcatatc gcgcatattg ctgccgccgc gcgtgaactg ttagatgcgg gcgtgcgcca
    21001 gcgggtggtg atccactgcc cggaaggggc gtggggagag accccagaac agggcggcgt
    21061 gtggatccct tcgcaaaagt tagaacaacg ggaaattatc ggcagcgtcg gcgcgggcga
    21121 tgcattttgt gccggatttt tatatggctg ccatgaacgc tggacgctta ccgagagcat
    21181 caaactggca cacgcctgcg cgcgggcaag cctgttatgc gccaatgcca tcgacggggc
    21241 gaaaacgctg gaagagctgc aaacagaaat gtcggattaa tgcacgtcgg cagcgaagac
    21301 ataccccaga ccgcgcagag ttttaatcaa catcggctga tgcgggttga gttcgatttt
    21361 gcgtcgtaat cgcatgatta atacgtcgat ggtgcgatca aacacctcca cgctgtcgct
    21421 gtgggtgaac gccagcaact gctcgcggct caatacccga cgcgcgtttt gcgccagagc
    21481 cagtaacaag ccatattcgc cctgagttaa ctcgacatgt tgctgaagcg gattgtgcaa
    21541 ctggcagcgc atggtatcga gataccagcc attgaatgac agcccggcgc ttttctcgtc
    21601 attaaccgcc agcgcaccaa cccgtcgcaa caccgctttc agtcgtgcga ccaccacccg
    21661 cggggtgaac ggtttgcaga tgtaatcatc cgcgcccatt tccagcccga ccaccacatc
    21721 agactcactg cccatgccgg tcaacatcac caccggcaat gtcggacgca gtttctggat
    21781 ttgctgcaat accctcaggc cgttggtatc gggtagcatc atatccagca ccaccagcgc
    21841 aatgtcagga tcgccctcaa tacgcaatat ggcctcctgt ccggtatggc agactgaaac
    21901 cgtaaagaca tgttcactta acacgtcctg caacagtgcg cagatagccg tgtcatcatc
    21961 caccaccagc acgacgggtt tcatggttac actccgcatc attcatctga ttaatcattg
    22021 tgcgtacttc ttgtcgaagt gaaagctgaa agtaatataa ataataaaaa tataagtcag
    22081 gtcacattat tgatgatagt aataaatatg ccgtactttt ccatcttcag cacaaat
//
LOCUS       DAZKFU020000058        22133 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZKFU020000058 DAZKFU020000000
VERSION     DAZKFU020000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.058"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..150)
                     /locus_tag="ACHOQQ_RS08785"
                     /old_locus_tag="pgaptmp_001757"
     CDS             complement(<1..150)
                     /locus_tag="ACHOQQ_RS08785"
                     /old_locus_tag="pgaptmp_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470022.1"
                     /translation="MPRTCILYAHPSTTAGCGVNALSGLRMAREFVGLIRRVSVASGI
                     CARLPD"
     gene            complement(157..1170)
                     /gene="mhpE"
                     /locus_tag="ACHOQQ_RS08790"
                     /old_locus_tag="pgaptmp_001758"
     CDS             complement(157..1170)
                     /gene="mhpE"
                     /locus_tag="ACHOQQ_RS08790"
                     /old_locus_tag="pgaptmp_001758"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGU4470023.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(1167..2117)
                     /gene="mhpF"
                     /locus_tag="ACHOQQ_RS08795"
                     /old_locus_tag="pgaptmp_001759"
     CDS             complement(1167..2117)
                     /gene="mhpF"
                     /locus_tag="ACHOQQ_RS08795"
                     /old_locus_tag="pgaptmp_001759"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGU4470024.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLDANVEQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(2114..2923)
                     /gene="mhpD"
                     /locus_tag="ACHOQQ_RS08800"
                     /old_locus_tag="pgaptmp_001760"
     CDS             complement(2114..2923)
                     /gene="mhpD"
                     /locus_tag="ACHOQQ_RS08800"
                     /old_locus_tag="pgaptmp_001760"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGU4470025.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(2933..3799)
                     /gene="mhpC"
                     /locus_tag="ACHOQQ_RS08805"
                     /old_locus_tag="pgaptmp_001761"
     CDS             complement(2933..3799)
                     /gene="mhpC"
                     /locus_tag="ACHOQQ_RS08805"
                     /old_locus_tag="pgaptmp_001761"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGU4470026.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            complement(3817..4761)
                     /gene="mhpB"
                     /locus_tag="ACHOQQ_RS08810"
                     /old_locus_tag="pgaptmp_001762"
     CDS             complement(3817..4761)
                     /gene="mhpB"
                     /locus_tag="ACHOQQ_RS08810"
                     /old_locus_tag="pgaptmp_001762"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGU4470027.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(4763..6427)
                     /gene="mhpA"
                     /locus_tag="ACHOQQ_RS08815"
                     /old_locus_tag="pgaptmp_001763"
     CDS             complement(4763..6427)
                     /gene="mhpA"
                     /locus_tag="ACHOQQ_RS08815"
                     /old_locus_tag="pgaptmp_001763"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGU4470028.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            6618..7451
                     /gene="mhpR"
                     /locus_tag="ACHOQQ_RS08820"
                     /old_locus_tag="pgaptmp_001764"
     CDS             6618..7451
                     /gene="mhpR"
                     /locus_tag="ACHOQQ_RS08820"
                     /old_locus_tag="pgaptmp_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGU4470029.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            7566..8342
                     /locus_tag="ACHOQQ_RS08825"
                     /old_locus_tag="pgaptmp_001765"
     CDS             7566..8342
                     /locus_tag="ACHOQQ_RS08825"
                     /old_locus_tag="pgaptmp_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGU4470030.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEVTMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIVA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            8362..9315
                     /locus_tag="ACHOQQ_RS08830"
                     /old_locus_tag="pgaptmp_001766"
     CDS             8362..9315
                     /locus_tag="ACHOQQ_RS08830"
                     /old_locus_tag="pgaptmp_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HGU4470031.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKHFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            9352..10434
                     /gene="lacI"
                     /locus_tag="ACHOQQ_RS08835"
                     /old_locus_tag="pgaptmp_001767"
     CDS             9352..10434
                     /gene="lacI"
                     /locus_tag="ACHOQQ_RS08835"
                     /old_locus_tag="pgaptmp_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGU4470032.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEYLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            10557..13631
                     /gene="lacZ"
                     /locus_tag="ACHOQQ_RS08840"
                     /old_locus_tag="pgaptmp_001768"
     CDS             10557..13631
                     /gene="lacZ"
                     /locus_tag="ACHOQQ_RS08840"
                     /old_locus_tag="pgaptmp_001768"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGU4470033.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFSAPEAVPESWLECDLPVADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLRGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPQPESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGRGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLPDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            13683..14936
                     /gene="lacY"
                     /locus_tag="ACHOQQ_RS08845"
                     /old_locus_tag="pgaptmp_001769"
     CDS             13683..14936
                     /gene="lacY"
                     /locus_tag="ACHOQQ_RS08845"
                     /old_locus_tag="pgaptmp_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGU4470034.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            15002..15613
                     /gene="lacA"
                     /locus_tag="ACHOQQ_RS08850"
                     /old_locus_tag="pgaptmp_001770"
     CDS             15002..15613
                     /gene="lacA"
                     /locus_tag="ACHOQQ_RS08850"
                     /old_locus_tag="pgaptmp_001770"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HGU4470035.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSDV
                     EKRESLIKEMFATVGENAWVEPPVYFSCGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(15716..16897)
                     /gene="cynX"
                     /locus_tag="ACHOQQ_RS08855"
                     /old_locus_tag="pgaptmp_001771"
     CDS             complement(15716..16897)
                     /gene="cynX"
                     /locus_tag="ACHOQQ_RS08855"
                     /old_locus_tag="pgaptmp_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGU4470036.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTQVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLLLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(16903..17373)
                     /gene="cynS"
                     /locus_tag="ACHOQQ_RS08860"
                     /old_locus_tag="pgaptmp_001772"
     CDS             complement(16903..17373)
                     /gene="cynS"
                     /locus_tag="ACHOQQ_RS08860"
                     /old_locus_tag="pgaptmp_001772"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HGU4470037.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(17404..18063)
                     /gene="cynT"
                     /locus_tag="ACHOQQ_RS08865"
                     /old_locus_tag="pgaptmp_001773"
     CDS             complement(17404..18063)
                     /gene="cynT"
                     /locus_tag="ACHOQQ_RS08865"
                     /old_locus_tag="pgaptmp_001773"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HGU4470038.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            18173..19072
                     /gene="cynR"
                     /locus_tag="ACHOQQ_RS08870"
                     /old_locus_tag="pgaptmp_001774"
     CDS             18173..19072
                     /gene="cynR"
                     /locus_tag="ACHOQQ_RS08870"
                     /old_locus_tag="pgaptmp_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HGU4470039.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAVHEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHTALEECADVGENESR"
     gene            complement(19412..20695)
                     /gene="codA"
                     /locus_tag="ACHOQQ_RS08875"
                     /old_locus_tag="pgaptmp_001775"
     CDS             complement(19412..20695)
                     /gene="codA"
                     /locus_tag="ACHOQQ_RS08875"
                     /old_locus_tag="pgaptmp_001775"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HGU4470040.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKICAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(20685..21944)
                     /gene="codB"
                     /locus_tag="ACHOQQ_RS08880"
                     /old_locus_tag="pgaptmp_001776"
     CDS             complement(20685..21944)
                     /gene="codB"
                     /locus_tag="ACHOQQ_RS08880"
                     /old_locus_tag="pgaptmp_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGU4470041.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGLADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         5228 a         6119 c         5815 g         4971 t
ORIGIN      
        1 atccggcagt cgtgcgcaga tgcctgatgc gacgctaacg cgtcttatca ggcctacaaa
       61 ttctcgcgcc atccgtaggc cggataaggc gttcacgccg catccggcag tcgtgctggg
      121 atgggcatac aaaatgcacg ttctcggcag tcaatattat ttgttgttgc gcagatccag
      181 cgccacgtcg acgatcatat cctcctggcc gccaaccatc cggcgtttgc ccagctcaac
      241 gagaatatcc accgcgctta agccataacg cgccgccgcc gtttcacagt gacgcaggaa
      301 gctggagtaa acaccagcgt atcccagcgc cagcgtttcg cgatcgactc gtaccggtcg
      361 atcctgcaac ggacgcacca ggtcgtcagc ggcatccatt aacgcataga gatcggcccc
      421 atgctgccaa cccagtttat ccacggcggc aataaatact tccagcggcg cgttacctgc
      481 gcccgctccc attcccgcga ggctggcgtc gattcggtcg cagccctctt ccaccgccgc
      541 gatagagttc gccacgccaa gactcaggtt atggtgagcg tgcatgccgg tttgcgtttc
      601 tggtttcagc actgctttca gggcgcggaa acggtcacgg atatcgctca tgttcatcgc
      661 accgccagaa tccaccacat aaatgcaggt cgcaccgtag ccttccatca gctttgcctg
      721 cttggcgaga ttctccggcg tggtcatatg gctcatcatc agaaaaccaa cggtgtccat
      781 tccgagctcg cgggcatact gaatatgctg ggcggaaaca tcagcttcgg tacagtgcgt
      841 agccacacga accacccgcg cgccagcctg ccaggcattt ttcagatcgt gaatagtgcc
      901 gattcctggc agcaacaacg tcgcgatttt ggcgtgcttc accacatccg ccgctgcttc
      961 aatccattca aggtcgctat gtgcgccgaa accatagtta aagctggacc cctgcaaacc
     1021 gtcgccgtgg gccacttcaa tcgaatccac gcgggcatcg tccagtgctt tggcaatctg
     1081 gcgaacgttt tccagcgaat actgatgacg aatggcgtgc ataccgtcac gcaacgtgac
     1141 gtccgatata taaagttttt taccgttcat gccgcttctc ctgccttgcg cgccagtgac
     1201 tgggccattt tttccgctgt cgccagcgca ctggaagtca taatgtcgag gttgcccgca
     1261 taggcaggca gataatgcgc tgcgccttcg acttccagcc agaccgctgt tttcagtccg
     1321 gagaattgcc ccacgcccgg taaattgacc ggtttatcct gcgggataac ttcaaactgc
     1381 acgcgctgtt tcaggcgata tcccggtacg taagcctgca ccgcctcagc catttcattg
     1441 attgaggctt cgatatcatc ttgtgaagct tcgtcgctca atacatacac cgtgtcacgc
     1501 atcatcaacg gtggctctgc tgggttaagc acgataattg ccttcccttt tgccgcgccg
     1561 cccaccactt caatggctcg ggaagtggtt tccgtaaatt catcgatatt ggcacgcgtg
     1621 ccaggtccgg cagatttact ggcgatagaa gcgataattt cggcgtaatg aacacgcgcc
     1681 acgcgtgaaa ctgccgccac cattggaatg gtggcctggc cgccgcaggt gaccatattg
     1741 acgttcagtt gttcgacatt cgcatcaagg ttaaccaccg gcacgcagta agggccgatg
     1801 gcagcaggcg tcaggtcgat taagcgaata tccggtttcg cttcgcgcaa agcggcatcg
     1861 tttttcacat gtgcgcccgc gctggtcgca tcaaatacaa tgtcgatatc agcaaattca
     1921 ggcatgttca tcagcccgat caccccttca tgggtggtgg cgacgcccat acgtctggca
     1981 cgcgccagac cgtcggactg aggatcaata ccgaccatca ccgccatctc cagatgctga
     2041 ccgtgacgca aaattttaat catcagatcg gtgccaatgt tgccagaacc gataatggcg
     2101 actttacgct tactcatgac agacttcctt ttggggctgc gcttgaaaat gtcgcagcaa
     2161 ctgaacctat gccttcaata tgggcttcaa aacgatcgcc cgcattcact gccaccatcg
     2221 gacctaatgc cccggtaaga atgatatctc cggcgcgcag cggttcaccc agactggcca
     2281 ttttgcgtgc cagccagacg gcggcattaa gcggatgtcc caggcattcg ctgccgcgcc
     2341 cgctggaaac ctcttcgtta ttacgcgtca tcttcatggc gcagtttttc aggtctaacc
     2401 ccgccggacg ttgcgccgga ccgccgatga catacacccc gcaggaagcg ttatctgcca
     2461 cggtatcgac aaactgaatc gaccagtcgc gaatgcggct acccaccacc tccagcgccg
     2521 gaagtaccca ttcaatggcg ttatacaatt cgtcgaaggt gatatcggtt gcgggcaaat
     2581 cgcggttcaa caccagtgcg atctccgctt caatgcgggg ttgcagaaca cgggaaaaag
     2641 gaatgatttc gttatcgcca taacacatgt cggcaaataa tgtcccgaaa tccggttgat
     2701 caacgcccag ttgttgctgc actttcggat gtgtcaggcc cactttacgc cccaccacgc
     2761 gacgcccctg cgcaacgtca tattgcacat ttatgtgctg aatggcgtag gccgcttcag
     2821 cgttatcgat accaatcaga tcgcgcagcg gcgcaatcgc ttcgccctgc tctgcggcgc
     2881 ggcgtaaatc cgccgccagt tgctcaagag tatgcttcgt catgaccgtt ccttaagcac
     2941 gtgcgaggaa attcagcacc agttgattga aagcgtcggc atgttcccac tgcgcccaat
     3001 gaccacagtc gcggaagata tgcagttcag aaccggcaat gccggacagc agacgcagac
     3061 ccgcatccat cggcacaaag cggtcgttgc gcccccagac aatcagggtt tgcgctttga
     3121 tttccgccag acgtgggcca aaatccggga actgtttcgg attagcttcc aggctcttaa
     3181 cgaagttttc caggtgatcg cggcgcgaca gcatattatt caggcgcgct tcaaacaggg
     3241 cgtcggtcaa atcgctggta tcaaaaacga agatatccat catcagcttc aggttttcga
     3301 tagtcggctg acgataaagc tgattcagtc gcttaatacc ttcggttggc atcggcgtaa
     3361 acaaactcat gccgcccgta ccgccgccca tcagcaccag tttgccgacg cgctccggcc
     3421 agttaagggt gaacgccaca gaactatggc cacccatcga gttgcccagc aggtggattt
     3481 tggcgatatc cagttgatcc accacgcttt tcaggattcg tgcattaaga tccgatcgcg
     3541 aaccactatt aacgatcgaa tcgctcttgc cccaacccgg acaatccagc aggatcaccc
     3601 gatagcccgc ctctaccagc ggatcgatat tgcggctgaa gttcgcccag ccagtagcac
     3661 ccgggccgga accatgcagc aggacaacgg tttcgtcgcc ttgtccgcag tcattaaaat
     3721 ggatgcgcag cgttttaccc gcttcttcta cattcagaaa acggctggtg gcggcttcgg
     3781 tttgtggctg ataactcata atcttctcct gcatattcag ttctctgttc tggcgcttaa
     3841 cgagccaaat ccggcaatcc actccgggat tgggcggtaa taacgccctt cgctacgcca
     3901 gttgccaaat gcagaaatag cggcaaaagc ggcgacccag gttttgattt catgtgtcga
     3961 ctttccggca atggcggaaa gctcttcgtt actgacggca tccagttcct gtatgcgtcc
     4021 ctgctccagc aaagtcatga actggttatc ccaaatcggg ttgagcggat gcagagttct
     4081 ctgatcctca acaaacttct cagcggcgct aatcaccctt tgctgacgca attcgcgctc
     4141 actggcgggt aaatctttcc cgctccccaa cagacggtcg cgcatatggg catcggcttt
     4201 cgccagttcg ggcaccggcg gctgatggga aagcccaccg gaacccagga acagcacgcg
     4261 tttattgaga gtgctggtga aacgtccaat ggcttcaccc aacatgcggg tacgctggaa
     4321 accgggcagc ggcgtggcga caccgttgat gaacacaggc agaactggca ccttatccag
     4381 cccaccgagc aggaactcca gcggctgtgc gaacccgtgg tccacctgca tacagtaaga
     4441 aacggcaaga tcgatcccgc tcttcatgac ggcatgcgca caggcctccg ccagctccac
     4501 aggcactggc agctctcctg ccgcactgcc gaaatcacca attgccgtcg ctccaacgcc
     4561 taaacagaac ggtggcatca cgtcatagaa aaagccgttg tagtgatctg gcgcaaacag
     4621 caccaccagt tcaggggaga aagccgcaat acgctcgcgg gcgctggcaa tcacgccatt
     4681 gacctcatcg agtacctctt gtgctgggtc gacatacccc accagcggcg agtgggaaag
     4741 acaatgaaga taagcgtgca tatcaggcta ccttttcgac agaaacgtcg gcatcagggc
     4801 gggtcagcgt catcaccgac gccagtttat tcagggtctt gcccagggtt tgcggaatgg
     4861 cggtggcggc aacaaagcga tccgggcgca tcaccaccag cgaagcattg tgttgcgcga
     4921 accagctacg caggcgacct tgcgtatccc ccacgcgtag tacgccgtcg tggttatcct
     4981 gtgcggtatg aatttgcact tccggcacca cctgaatgaa gcgtgtgccc aacgcgcgcc
     5041 actgctggat ttgctcgtcg ctcatccccc acagtggatt gcatccccag ccaattaccg
     5101 cgaagttcgc gccgatcgcg ttatcgagta gcgtcacgtc gccgttttcc agcgtgactt
     5161 tcggctgaat aaacatcttg ccgaccggag agtgcttcgc ttcgccctca cgtaccagcg
     5221 caccgccgta atattgcggc atcggcttga agcgcatttc gaggaagtag cgttttactg
     5281 gcggcagata attcagcagc caggaaacgc cgtcacgtaa cgtaccctgc cagcgtttcg
     5341 gcggagccag cacgttgccc gccgtcacgg acaggtcaat catcgctttg gcgtgatcgc
     5401 gacgttcttg ttgataggta tcgagcagcg catcgcgggc tttcccctgg ataaccaacg
     5461 ccagtttcca tgcgaggtta aaagcgtcgc gcatgccact gttatagccc tgcccctgcc
     5521 ataccggcat gatgtgcgcg gcatcgcccg ccagcagtac gcgatcaata cggaaacgtt
     5581 gcgccagtcg cgcgttgtgg gtgtagacac gctggcgaat caattcaaca ttgtccggat
     5641 taggcagcac tttgcttaac agcttgcgca tattttgcgg ctcacgcagc tgttcttcgg
     5701 tttctcctgg catcaccata aattcaaagc gacgtaccgc atgaggtagc gcggcagaaa
     5761 catacgggcg caccggatcg caacacaaat agatatgcgg cgtacttaac ggatcgttgg
     5821 cgatatctac cacaatccac tgatttggcg cagttttacc ttcaaacggc acattcaggg
     5881 tgcgacggac aaaacttgcc ccaccatcac aggccaccag ccactgggct ttgactattt
     5941 cccgctgccc ttctgccgtt ttcaggtgca aggtcacttc gtcatcttgc tgactgaagg
     6001 cctccagctc gcgggaaaac aagcagcgca cattcggaaa acgcgacacc ccttccagca
     6061 tcaccgcatc gacctgcggc tgaataaagg cgttacggcg cggccagcca aattcatcgg
     6121 tcattggctg aatatcagca aaacagcggc ccttcggggt gagaaaacgc atcgcgtgcc
     6181 acggcgtagt atgcggcaga acattatcga ccaggccgac cgactgcatg gtgcgcagcg
     6241 cctcgtcatc aataccaatc gcacgcgggt agtcgatcaa cttatcgagt ttctccacca
     6301 ccagcacgtc aatgcccatc tggccgagat agttcgccat catcagcccg accggaccgg
     6361 caccagcgat cgccacctga acgctatggt taacagcagg ctggatgtca gggtgttgta
     6421 ttgccatttc agtacctcac gactcggaca aaatgccgtt gcgcgcacag tacagcgcaa
     6481 cttattttgt taaaaacatg taaatgattt tttattgtgc gctcagtata gaaagggtgt
     6541 tttcggctac aatcaaaaca tgcccgaatg tgcaccaggt gcacaacgtt gttttaacta
     6601 tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc gtgcgcggct
     6661 taacccgcgg tctaatgtta ttaaatatgt taaataaact tgacggcggt gccagcgtcg
     6721 ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg ctggagacgc
     6781 tgcaggaaga gggatatgtc cggcgtagtc cctccgatga tagttttcgt ctgaccatca
     6841 aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca ctggcggccc
     6901 cactgctggg cgatctgttg cgcgaagtgg tatggccgac agatgtgtcc acgctggatg
     6961 ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc tttcaccggg
     7021 caatggtcgg gcgacgtttg ccgcttctga aaaccgcctc gggcctgacc tggctggcct
     7081 tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agcctcccgc cccggtgatg
     7141 actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgt gcgcgcaaag
     7201 agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc tctatcgccg
     7261 taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat atggcgagcg
     7321 caatgaccat cgaacaggca gcggaaaagc atcttccggc actacaacgg gtagcaaaac
     7381 agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga agcggcgtgc
     7441 atttacgcta attacgccaa tgtgtaattt ggcccattct catcgttatt cgcccatttg
     7501 cccacatgca cagtacgggc tgcacgctac gctaatgaca tcaatactgt gatgtggagc
     7561 aaatcatgaa aattacgcaa attcgtaatg ccacccaaat tatccaatac gctggcaaaa
     7621 catttcttat cgacccgtta ctcgcgccta aaggaagtta tccgggtttt gccggaacgg
     7681 cacgcgcaga aatccgcaat ccaacggtag aattaccgtg cgatttaaca acgttactca
     7741 acgttgacgc cctaatcgta acccatttgc atgaagatca ctgggacgaa acagcctccc
     7801 gcgtcgtccc taaagataaa ctgatttatg tgcaaaatga gcaagatgct gaatcactga
     7861 ataaacaagg atttacgcat cttaccgtac ttaccgacga gactaaattt ggcgatatta
     7921 ctctgcataa aacgacaggg cagcacggct ccgatcgcac ctatgccgac ccggctatgg
     7981 cgaagcgttt aggcgcagcc tgcggcgttg ttttccagca cccgacagag aaaacgctct
     8041 atctggctgg cgataccctc tggcgtgacg acgtcgaggt tacaatgcta actttccaac
     8101 cagaagtggt tgtgttgaat gccggatttg cgcatgttct ggggcatggc gcgataatta
     8161 tgggagcaga agatattctc aaaacacatt ttaccctgcc agaagcgcaa attgtcgcga
     8221 cacatatgga ggccgtaaac cactgtttgc tgacgcgtga tgcgctcaag gaatacgcca
     8281 gagataatca aattatcgaa tttgtgaacg taccagaaga tggtgaaacg ttgacattct
     8341 aattcggaga taaagagtgt tatgcgcccg ctaactgttg ccattatcgc cgttgccggt
     8401 tttagtcctt ttcacctttc cgtaccgttt atcgtattta gcgaaaaaat ggcggagaaa
     8461 aaacactttc acgtaattat ctgcgctgaa aagccgggaa acgtggactc tgccgatggg
     8521 ttttccgtaa ccgccaccca tgactatacc gcagtcatcc aggcggatat tgtgataatt
     8581 ccttactggg gaaccattac acaaaaaccg ccacaaaaac tgctggaagc cttaacgacc
     8641 gcacgggata acggcgcaca aattgtcggg ctttgcctgg gcacgtttgt gctcggctat
     8701 gcaggtttac tgaaaaataa gcgtgccgcc acgcactggg agttcgagcg tgaatttcag
     8761 gcacgttttc cacaaacaca tctggatatt aacgcgttgt acgtagacga tgacggcatt
     8821 attacctctg ccggtactgc cgcggcgctg gattgctgtt tgtatattgt tcggcaacat
     8881 tttggcagcg actatgctaa ccatattgcc cgacggatgg tcgtaccgcc atatcgcacc
     8941 ggcggtcagg cgcagtttat tgagcaacca gtgccgaaaa atacccatga tgaacgcatt
     9001 aatctcctgc tggattacct gcggcaaaac attgcgcaac agcatgatct cgactcgctg
     9061 gcgcagcgag taatgatgag tcgccgcaca ttaactcgcc attttatgaa agcgaccggt
     9121 tcgagtatcg ccgaatggct cattactgaa cgcttacgcc gtagccagga actgttggga
     9181 tccagtcagt tgcccgttga gcggatagcg gctgaggtgg gttttctctc acctgtgacc
     9241 tggcgtcagc attttaaatc tcacttcggc gtcagccccg ccgaatggcg caaaaccttt
     9301 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
     9361 gtaacgctat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
     9421 gtgaatcagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
     9481 gagctgaatt acattcccaa ccgcgtggca caacaactgg cggggaaaca gtcgttgctg
     9541 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     9601 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     9661 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     9721 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cgctaatgtt
     9781 ccggcgttat ttcttgatgt ctctgaccag actcccatca acagtattat tttctcccat
     9841 gaagacggta cgcgactggg cgtggagtat ctggtcgcat tgggtcacca gcaaatcgcg
     9901 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggcggg ctggcataaa
     9961 tatctcactc gcaatcaaat tcagccgata gcggaacggg agggcgactg gagtgccatg
    10021 tccggttttc aacaaaccat gcaaatgcta aatgagggca tcgttcccac tgcgatgctg
    10081 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggttgcgc
    10141 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc gtgttatatc
    10201 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
    10261 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
    10321 gtgaaaagaa aaaccaccct ggcgcccaag acgcaaaccg cttctccccg cgcgttggcc
    10381 gattcattaa tgcagctggc acgacaagtt tcccgactgg aaagcgggca gtgagcgcaa
    10441 cgcaattaat gtgagtcagc tcactcatta ggcaccccag gctttacact ctatgcgtcc
    10501 ggctcgtatg ttgtgtgaaa ttgtgagcgg ataacaattt cacacaggat acagctatga
    10561 ctatgattac ggattcactg gccgtcgtat tacaacgtcg tgactgggaa aaccctggcg
    10621 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    10681 aggcccgcac caatcgccct tcccaacagt tgcgcagcct gaatggtgag tggcaatttg
    10741 tctggttttc ggcaccagaa gcggttccgg aaagctggct ggagtgcgat cttcctgtcg
    10801 ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgacgcg cccatctaca
    10861 ccaacgtgac ctatcccatt acggtcaatc cgccatttgt tcccacggag aatccgacgg
    10921 gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa
    10981 ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt
    11041 acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag
    11101 aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg
    11161 atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcacaaa ccgaccacac
    11221 aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc gcggttctgg
    11281 aggcagaagt tcagatgtac ggcgagctgc gcgatgagct gcgggtgacg gtttctttgt
    11341 ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg
    11401 agcgtggcgg ttatgccgat cgcgtcacac taggtctgaa cgtcgaaaac ccgaaactgt
    11461 ggagcgccga aatcccgaat atctatcgtg cggtggttga actgcacacc gccgacggca
    11521 cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtacggatt gaaaatggcc
    11581 tgctgctgct gaacggcaag ccgttgctga ttcgcggcgt taaccgtcac gagcatcatc
    11641 ctctgcgtgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc
    11701 agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacaccc
    11761 tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg
    11821 tgccaatgaa tcgtctgacc gatgatccgc gctggctacc cgcgatgagc gaacgcgtaa
    11881 cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg
    11941 agtcaggcca cggcgctaat cacgacgcac tctatcgctg gattaaatct gtcgatccat
    12001 cccgcccggt gcagtatgaa ggcggcggag ccgacacctc cgcaaccgat attatttgcc
    12061 cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca
    12121 aaaaatggct ttcgttgcct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg
    12181 cgatgggtaa cagtcttggc ggcttcgcta aatactggca ggcgtttcgt cagtaccccc
    12241 gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa
    12301 atggcaatcc gtggtcggct tacggcggtg attttggcga tacgccgaat gatcgccagt
    12361 tctgcatgaa cggtctggtc tttgccgacc gcacgccgca tccggcgctg acggaagcaa
    12421 aacaccagca gcagtttttc cagttccgtt tatccgggcg aaccatcgaa gtgaccagcg
    12481 aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggca
    12541 agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa caggtaattg
    12601 aactgcctga actaccgcag ccggagagca ccgggcaact ctggctaacg gtacacgtag
    12661 tgcaaccgaa cgcgaccgca tggtcagaag ccggacacat cagcgcctgg cagcagtggc
    12721 gtctggcgga aaacctcagc gtgacactcc cctccgcgcc ccacgccatc ccgcatctga
    12781 ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc
    12841 agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc
    12901 gcgatcagtt cacccgcgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca
    12961 ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcgg
    13021 cgttgttgca gtgcacggca gatacacttg ccgacgcggt gctgattacg accgctcacg
    13081 cgtggcagca tcagggaaaa accttattta tcagccggaa aacctaccgg attgatggta
    13141 gaggtcaaat ggcgattacc gttgatgttg aagtagcgag cgatacaccg catccggcac
    13201 ggattggcct gacctgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag
    13261 ggccgcaaga aaactatccc gaccgcctta ctgctgcctg ttttgaccgc tgggatctgc
    13321 cattgccaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga
    13381 cacgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc
    13441 gctacagcca acaacaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag
    13501 gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc
    13561 cgtcagtgtc ggcggaattc cagcttagcg ccggtcgcta ccattaccag ttggtctggt
    13621 gccaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta aggaaatcca
    13681 ttatgtacta tttaaaaaac acaaactttt ggatgttcgg tttattcttt ttcttttact
    13741 tttttatcat gggagcctac ttcccgtttt tcccgatttg gctacatgac atcaaccata
    13801 tcagcaaaag tgatacgggt attatttttg ccgctatttc tctgttctcg ctattattcc
    13861 aaccgctgtt tggtctgctt tctgacaaac tcgggctgcg caaatacctg ctgtggatta
    13921 ttaccggcat gttagtgatg tttgcgccgt tctttatttt tatcttcggg ccactgttac
    13981 aatacaacat tttagtagga tcgattgttg gtggtattta tctaggcttt tgttttaacg
    14041 ccggtgcgcc cgcagtagag gcatttatcg agaaagtcag ccgtcgcagt aatttcgaat
    14101 ttggtcgcgc gcggatgttt ggctgtgttg gctgggcgct gtgtgcctcg attgtcggca
    14161 tcatgttcac catcaataat cagtttgttt tctggctggg ttctggctgt gcactcatcc
    14221 tcgccgtttt actctttttc gccaaaacgg atgcgccctc ttctgccacg gttgccaatg
    14281 cggtaggtgc caaccattcg gcatttagcc ttaagctggc gctggaactg ttcagacagc
    14341 caaaactgtg gtttttgtca ctgtatgtta ttggcgtttc ctgcacctac gatgtttttg
    14401 accaacagtt tgctaatttc tttacttcgt tctttgctac cggtgaacag ggtacgcggg
    14461 tatttggcta cgtaacgaca atgggcgaat tacttaacgc ctcgattatg ttctttgcgc
    14521 cactgatcat taatcgcatc ggtgggaaaa acgccctgct gctggctggc actattatgt
    14581 ctgtacgtat tattggctca tcgttcgcca cctcagcgct ggaagtagtt attctgaaaa
    14641 cgctgcatat gtttgaagta ccgttcctgc tggtaggctg ctttaaatat attaccagcc
    14701 agtttgaagt gcgtttttca gcgacgattt atctggtctg tttctgcttc tttaagcaac
    14761 tggcgatgat ttttatgtct gtactggcgg gcaatatgta tgaaagcatc ggtttccagg
    14821 gcgcttatct ggtgctgggt ctggtggcgc tgggcttcac cttaatttcc gtgttcacgc
    14881 ttagcggccc cggcccgctt tccctgctgc gtcgtcaggt aaatgaagtc gcttaagcaa
    14941 tcaatgtcgg atgcggcgcg agcgccttat ccgaccaaca tatcaggacg gaatgagcga
    15001 aatgaacatg tcgatgacag aaagaataaa agcaggcaag ctatttaccg atatgtgcga
    15061 aggcttacct gaaaaaagac ttcgtgggaa aacattaatg tatgagttta atcactcgca
    15121 tccatccgac gttgaaaaaa gagaaagcct gattaaagaa atgtttgcca cggtagggga
    15181 aaacgcctgg gtagagcccc ccgtctattt ctcttgcggt tccaacatcc atataggccg
    15241 caatttttat gcaaatttca atttaaccat tgtcgatgac tacacggtaa caatcggtga
    15301 taacgtactg attgcaccca acgttactct ttccgttacg ggacaccctg tacaccatga
    15361 attgagaaaa aacggcgaga tgtactcttt tccgataacg attggcaata acgtctggat
    15421 cggaagtcat gtggtcatta atccaggcgt caccatcggg gataattctg ttattggcgc
    15481 gggtagtgtc gtcataaaag acattccacc aaacgtcgtg gctgctggcg ttccttgccg
    15541 ggttattcgc gaaataaacg accgggataa gcaatattat ttcagagatt acaaagttga
    15601 atcgtcagtt taaattagca aaattgcctg atgcgcttcg cttatcaggc ctacaaattc
    15661 agtgttctac gttagccgca tccggcatga acaaagcgca ggaacaagcg tcgcatcatg
    15721 cctctttgac ccacagctgc ggaaaacgtg ctggtgcaaa acgcagggtt atgatcatca
    15781 gcccaacgac gcacagcgca tgaaatgccc agtccatcag gtaattgccg ctgatactac
    15841 gcagcacacc agaaaaccac ggggcaagcc cggcgatgat aaaaccgatc ccctgcataa
    15901 acgccaccag cttgccagca atagccggtt gcgcagagtg atcgagcgcc agcagcaaac
    15961 agagcggaaa cgcgccgccc agacctaacc cacacaccat cgcccacaat accggcaatt
    16021 gcagcggcag ccagataaag ccgcagaaac ccaccagttg taacgccagt gccaacagca
    16081 aaagtttgcg ccgatcctga tggcgagcca tagcaggcat tagcaaagct cctgcggctt
    16141 gcccaagcgt catcaatgcc agcaaggaac cgctgtactg cgcgctggca ccaatctcaa
    16201 tatagaaagc gggtaaccag gcaatcaggc tggcgtaacc accgttaatc agaccgaagt
    16261 aaacacccag cgtccacgcg cgaggagtga aaaccacgcg aacctgagtg gttgttgtct
    16321 tgtgggaaga ggcgacctcg cgggtgcttt gccaccacca ggcaaagagc gcaacaacgg
    16381 caggcagcgc ccaccaggcg agtgtttgat accaggtttc gctatgttga actaaccagg
    16441 gcgtcagggc ggcaccaagt ccaccgccgc ccatcagagc cgcggaccac agccccatca
    16501 ccagtggcgt gcgctgctga aaccgccgtt taatcaccga aggcatcacc gcctgaatga
    16561 tgccgatccc caccccacca agcagtgcgc tgctaagcag cagcgcactt tgggggtaaa
    16621 gctcacgcat caatgcaccg acggcaatca gtaacaaact gatggcgacg ctgcgacgtt
    16681 cgctgacatg ctgatgaagc cagcttccgg ccagcgccag cccgcccatg gtgaccaccg
    16741 gcagagcggt caacagggca gccacgctaa agctcattcc gcttgcctgg cgcaattgcg
    16801 gtagcagtgg cccgacggag gtgagcagtg gtcgcatatt aagaccaatc agcaccagaa
    16861 ccagcagcat cgtgaacgtc ttttgatggt tacgcatggc tgtcagaacg gtttggtcgg
    16921 cagatattta ccatctaagg tgatgaccgc acgttcgcca ccttccgggt ccgccacttt
    16981 cttaacgtcg agtttgaagt taatcgcgct gataatgcca tcgccaaact tctcatgaac
    17041 caacgctttc agggttgtgc cgtacacctg caacatttca tagaaacgat acatcgttgg
    17101 gtcggttgga atacggtcat caatgcagcc acgcagtggg atcatctgta acagtagaat
    17161 ggcgtcttcg tcgagatcca gcttcgcccc gacctggcgg gcggcgtcgg caggaagcgc
    17221 ctgctgaccc agcaaagccg cggtcacaaa ggcttctgcc agcccggtgc cgtcggcaat
    17281 ctcggcaaat gagagatctt ttttagcttt gctgagcaaa atggcatcgg caagatcaag
    17341 acgaatattg cgattaattt gcgactgaat catggtgcat ctcctgatgg tttaaaaata
    17401 aggttacgct gcggtcggtt ggcgtagcgg tatggcacaa acgcgaggat tagcggccag
    17461 tggcacaaac tggcgggttg cgccgtcaaa cgctgcgatg ctgccgcttt caatgtcgta
    17521 gacccagccg tgcagggcga tccgcccctc ttcgagcgcc aggcgcaccg atggatgagt
    17581 ttgcaaatta gccaactgag caatgacgtt ttcacgtacc atcgccgcag cttttgacgg
    17641 taaatcggaa tgcgggcgcg cctcattaac gacgcgggct gaatcggcat aacgcagcca
    17701 gtgggagacg gcaggcatat ggtccatgca ctgacagctg gcaatggcgg tcatcgcgcc
    17761 acagttggaa tgaccgcaaa tcacaatgtc agatacccga agcgcagcga cggcatactc
    17821 caccgaagca gaaacgccac cgggttccgg cccgtaggaa gggacgatat tgcccgcgtt
    17881 gcgaataacg aacagatcgc caggctcacg ttgcgtcacc agctcaggga ccagacggct
    17941 gtcggagcag gagataaaaa gtgtgcgtgg gctttgctgt gtcgccaact gtttaaataa
    18001 ggcttcccgc ttcggaaatg cctcgcgctg gaatttaagg aatccatcaa taatctcttt
    18061 caccgttaac ctctgtctgt ctctggaatg agaggcagac tactcgccga ttgtcataag
    18121 gtaaaagtct catttatgat gagttctatt ggatttactt ataggttggc gaatgctctc
    18181 tcgacatatc aattattttc ttgccgtggc tgaacatggc agcttcaccc gtgccgccag
    18241 tgcgttgcac gtctctcagc ctgcgctttc ccagcagatt cgccagttag aggagagttt
    18301 aggcgtgccg ctgtttgacc gtagcgggcg aacgattcgt ctcactgatg caggagaagt
    18361 ctggcgacag tacgccagcc gggcgttaca ggaactggga gcgggtaaac gggcgattca
    18421 tgatgttgcc gatctgacga gaggatcgct gcgtatcgcc gtcaccccca cctttacgag
    18481 ctactttatc ggccccttaa tggcggattt ctatgcgcgc taccccggca tcacgctcca
    18541 gctacaggaa atgtcgcagg agaaaatcga ggatatgctt tgccgcgacg agttggacgt
    18601 tgggattgcc ttcgcgcctg tgcattcgcc ggagctggag gcaattcctt tactgacaga
    18661 aagtttagcg ttagtcgtgg cgcaacatca tccgctggcc gtccatgaac aggtggcgtt
    18721 gagtcgcttg catgatgaaa aactggtcct gctcagcgcg gagtttgcca ccagagagca
    18781 aattgaccac tactgcgaga aagcggggct gcatccacag gtggtcattg aggcgaactc
    18841 aattagcgcg gttctggagc tgattcgccg cacttccctt tccacattgt taccagcagc
    18901 gattgccaca caacatgacg ggcttaaagc tatttctctt gccccgccac tactggagag
    18961 aacggcggtt ttgttgcggc ggaaaaatag ctggcagaca gccgccgcga aggcattttt
    19021 gcacacggcg ttggaggaat gtgcggatgt tggcgagaat gaatcacggt agccactggc
    19081 acaggtctgc ggctggaatt gcctgatgcg ctacgcttat caggcctacg agttcagtgt
    19141 aaggcgttca cgccgcatcc atcataaact acagctggca gacagccgcc gcgaaatcat
    19201 ttttgcacat ggcattggaa gaatgcgcgg atgttggcgg gaatgaatca cggtagccgc
    19261 tgacacagat cagcggctgg aattgcctga tgcgctacgc ttatcaggcc tacgagttct
    19321 acgagttcag tgctctacgt aggccggata aggcgttcac gccgcatccg gcataaacta
    19381 cgctcgcttt gtcaacacac agcccagccg atcaccgttt gtaatcgatg gcttctggct
    19441 gctccagata tacggtggtt tgtgccggtt gtgtgctggc aatcaccttg ccgccacgta
    19501 ccgaataacg taccggaacc tgacggcgca gcgcatcaaa tccattttca gccggcagga
    19561 taatcaggtt ggcgctgttt ccggcggcaa tgccgtaatc ctgcaaattc aacgtcctgg
    19621 cgctgtggtg ggtgattaaa ttcaggccat cgttaatctg cccatagccc atcagctggc
    19681 aaacatgcag ccccatatgc agcacttgca gcatattcgc cgttcccagc ggataccacg
    19741 gatcgaagac atcatcgtga ccaaagcaga cgttaatgcc ggactccagc atctctttaa
    19801 cgcgcgtgat gccgcgacgt tttggatacg tatcgaaacg tccttgcaga tgaatattga
    19861 ccagcgggtt ggcgacaaag ttaataccgg acattttcag caagcggaac aggcgtgagg
    19921 tatacgcccc gttataggag tgcattgccg tggtgtggct ggcggtgact cgcgcgccca
    19981 tgccttcacg gtgcgccagg gcagcaacgg tttcgacaaa gcgcgactgc tcgtcatcga
    20041 tctcatcaca gtgaacgtcg atgagacggt cgtatttttg cgccaaggcg aaggttttat
    20101 gcagcgactc cacgccgtat tcacgggtaa attcaaaatg cggaatcgcc cccactacat
    20161 ctgcccctaa gcgtaacgcc tcttccagca acgcttcacc gttgggatac gacaaaatcc
    20221 cttcctgagg gaaggcgacg atttgcagat caatccacgg cgcgacttcc tgcttcactt
    20281 ccagcattgc tttcagcgca gttagcgttg catccgaaac atcgacatgg gtacgcacat
    20341 gctgaatgcc gttggcaatc tgccatttca gcgtttgcca tgcgcgttgt ttcacatcgt
    20401 catgggttaa taacgctttg cgctcggccc agcgttcaat gccttcaaac agcgtgccgg
    20461 actgattcca gttcggttgt ccggcggttt gcgtggtgtc caggtgaata tgtggctcca
    20521 caaacggcgg tataactaaa ccttgttcgg catccaggct gttttcagtt atgggcatca
    20581 cgccggattg cgcatcaatg gcgcagattt ttccgtcctg cagatgaatc tgccacagcc
    20641 cctctttgcc tggtaaccgg gcgttaataa ttgtttgtaa agcgttattc gacactgtta
    20701 gcctccacat gcgtcattgc tgctgtcgtt ttacgattca aaatcgggtt aaggatcaga
    20761 tagctcagcg cgccacctaa taccgcgttg accggaacaa ttcccggtaa ccagtggcct
    20821 gcagcaatcc ccagggcgac cgccagaatc gccacccaat tgacactcat catacgcgtg
    20881 gtcgcaaagt gctcatagcg gcgacggttc atcagatagt cggcaatgat cacgccaccc
    20941 actggaggaa tagctgccga aaggaaggtc aaccagccga caaaattgtt atacagccat
    21001 aatgcacaga ccgtaccgat aataccgttg attaccgaaa gggttttgct cgacatcccg
    21061 gtaatgttgg cgaaacctaa acccgacgca tagagcgcgt tatcgttggt ggtccagata
    21121 tttagcccca gcaccacaat cgcaggcaac agcaggccct gagcaatcat cacatcagag
    21181 atatccgcca accccagtgc cgccgctccc gctgcaccga aaataaacat caacgagttg
    21241 ccgaggaaaa aggccaccat cgccaccagc accgccagtt tggcattgcg accaaagcgg
    21301 acaaagtcag cggtgagcgt acccgcgctg ataaatgacc ccacaaccag cgccagcgcg
    21361 acattgaaat ctaatggttg tgcgggaacg accgctttta aggcgtccag gccgcccatg
    21421 ccgttaacgg ccagccacac ggaataaccg ccaagacagg cgatcgccgg aaccgcaatc
    21481 accgaaagaa ctgtcagcgc cgaaatgcca aagaagacgg tgacggtcat cagtaaaccg
    21541 gaaacggcaa tcagcaaatt aatatccagc ccggttgcct tacccaccgg aatggcaaac
    21601 atcgccacgc caacgccaaa ccagccaacc tgggtgccgc ccagcagcag tgaaggcagc
    21661 catgagcctt taacaccaaa cgagaagcga gcaagaagat gagtggtcag gccggttttt
    21721 gcgccaatgt aaccgagaaa tgaagtgtaa ataccgagga gaagattgcc gatgagaact
    21781 gcgaggaaga aatcatgata gctaagaccg gttccgagag tgccgccagt ccacatactg
    21841 gcggaaaaga aggttaatcc cagcatgacg aacgtcaatg ccaatacccc tttccgcgcc
    21901 gactgcggga ctggcccctg gctaaagttg ttatcttgcg acacgaaatt cccccttttt
    21961 gctgtttgaa acccaaaaaa tccgccgcat tctattcatc tgaagataaa aagcaatcgt
    22021 tttcgtgggg aaatatattt tttatatgaa tgagtgcaaa aaggtggggg aaaaattatc
    22081 ggtgtaaaaa cagtgggtag gcctgataag acgcgtcagc gtcgcatcag gca
//
LOCUS       DAZKFU020000059        22089 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZKFU020000059 DAZKFU020000000
VERSION     DAZKFU020000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.059"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..1756
                     /gene="ybaL"
                     /locus_tag="ACHOQQ_RS08885"
                     /old_locus_tag="pgaptmp_001777"
     CDS             80..1756
                     /gene="ybaL"
                     /locus_tag="ACHOQQ_RS08885"
                     /old_locus_tag="pgaptmp_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGU4470042.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(1886..3190)
                     /gene="gsk"
                     /locus_tag="ACHOQQ_RS08890"
                     /old_locus_tag="pgaptmp_001778"
     CDS             complement(1886..3190)
                     /gene="gsk"
                     /locus_tag="ACHOQQ_RS08890"
                     /old_locus_tag="pgaptmp_001778"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGU4470043.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            3342..4301
                     /gene="aes"
                     /locus_tag="ACHOQQ_RS08895"
                     /old_locus_tag="pgaptmp_001779"
     CDS             3342..4301
                     /gene="aes"
                     /locus_tag="ACHOQQ_RS08895"
                     /old_locus_tag="pgaptmp_001779"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGU4470044.1"
                     /translation="MKPENKLPVLDLISAEMKIVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNYADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(4298..5260)
                     /gene="hemH"
                     /locus_tag="ACHOQQ_RS08900"
                     /old_locus_tag="pgaptmp_001780"
     CDS             complement(4298..5260)
                     /gene="hemH"
                     /locus_tag="ACHOQQ_RS08900"
                     /old_locus_tag="pgaptmp_001780"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGU4470045.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(5392..6036)
                     /gene="adk"
                     /locus_tag="ACHOQQ_RS08905"
                     /old_locus_tag="pgaptmp_001781"
     CDS             complement(5392..6036)
                     /gene="adk"
                     /locus_tag="ACHOQQ_RS08905"
                     /old_locus_tag="pgaptmp_001781"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGU4470046.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(6217..8091)
                     /gene="htpG"
                     /locus_tag="ACHOQQ_RS08910"
                     /old_locus_tag="pgaptmp_001782"
     CDS             complement(6217..8091)
                     /gene="htpG"
                     /locus_tag="ACHOQQ_RS08910"
                     /old_locus_tag="pgaptmp_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGU4470047.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(8201..8806)
                     /gene="recR"
                     /locus_tag="ACHOQQ_RS08915"
                     /old_locus_tag="pgaptmp_001783"
     CDS             complement(8201..8806)
                     /gene="recR"
                     /locus_tag="ACHOQQ_RS08915"
                     /old_locus_tag="pgaptmp_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGU4470048.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(8806..9135)
                     /gene="ybaB"
                     /locus_tag="ACHOQQ_RS08920"
                     /old_locus_tag="pgaptmp_001784"
     CDS             complement(8806..9135)
                     /gene="ybaB"
                     /locus_tag="ACHOQQ_RS08920"
                     /old_locus_tag="pgaptmp_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGU4470049.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(9188..11119)
                     /gene="dnaX"
                     /locus_tag="ACHOQQ_RS08925"
                     /old_locus_tag="pgaptmp_001785"
     CDS             complement(9188..11119)
                     /gene="dnaX"
                     /locus_tag="ACHOQQ_RS08925"
                     /old_locus_tag="pgaptmp_001785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGU4470050.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(9791..9855)
                     /gene="dnaX"
                     /locus_tag="ACHOQQ_RS08925"
                     /old_locus_tag="pgaptmp_001785"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(11248..11799)
                     /gene="apt"
                     /locus_tag="ACHOQQ_RS08930"
                     /old_locus_tag="pgaptmp_001786"
     CDS             complement(11248..11799)
                     /gene="apt"
                     /locus_tag="ACHOQQ_RS08930"
                     /old_locus_tag="pgaptmp_001786"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGU4470051.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(11952..12329)
                     /gene="ybaN"
                     /locus_tag="ACHOQQ_RS08935"
                     /old_locus_tag="pgaptmp_001787"
     CDS             complement(11952..12329)
                     /gene="ybaN"
                     /locus_tag="ACHOQQ_RS08935"
                     /old_locus_tag="pgaptmp_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGU4470052.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12399..12926
                     /gene="priC"
                     /locus_tag="ACHOQQ_RS08940"
                     /old_locus_tag="pgaptmp_001788"
     CDS             12399..12926
                     /gene="priC"
                     /locus_tag="ACHOQQ_RS08940"
                     /old_locus_tag="pgaptmp_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGU4470053.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            12940..13101
                     /gene="rsmS"
                     /locus_tag="ACHOQQ_RS08945"
                     /old_locus_tag="pgaptmp_001789"
     CDS             12940..13101
                     /gene="rsmS"
                     /locus_tag="ACHOQQ_RS08945"
                     /old_locus_tag="pgaptmp_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGU4470054.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(13154..13252)
                     /locus_tag="ACHOQQ_RS08950"
                     /old_locus_tag="pgaptmp_001790"
                     /pseudo
     CDS             complement(13154..13252)
                     /locus_tag="ACHOQQ_RS08950"
                     /old_locus_tag="pgaptmp_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13313..16675)
                     /gene="mscK"
                     /locus_tag="ACHOQQ_RS08955"
                     /old_locus_tag="pgaptmp_001791"
     CDS             complement(13313..16675)
                     /gene="mscK"
                     /locus_tag="ACHOQQ_RS08955"
                     /old_locus_tag="pgaptmp_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGU4470055.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKIKVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(16803..17450)
                     /gene="acrR"
                     /locus_tag="ACHOQQ_RS08960"
                     /old_locus_tag="pgaptmp_001792"
     CDS             complement(16803..17450)
                     /gene="acrR"
                     /locus_tag="ACHOQQ_RS08960"
                     /old_locus_tag="pgaptmp_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGU4470056.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            17592..18785
                     /gene="acrA"
                     /locus_tag="ACHOQQ_RS08965"
                     /old_locus_tag="pgaptmp_001793"
     CDS             17592..18785
                     /gene="acrA"
                     /locus_tag="ACHOQQ_RS08965"
                     /old_locus_tag="pgaptmp_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGU4470057.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            18808..21957
                     /gene="acrB"
                     /locus_tag="ACHOQQ_RS08970"
                     /old_locus_tag="pgaptmp_001794"
     CDS             18808..21957
                     /gene="acrB"
                     /locus_tag="ACHOQQ_RS08970"
                     /old_locus_tag="pgaptmp_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGU4470058.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         5046 a         5787 c         5899 g         5357 t
ORIGIN      
        1 aaattgtcat aaactattac ctgtaatttt ggttttcccg gccaaaaatg gacaataacc
       61 accaggaaaa ggagacggaa tgcatcacgc caccccgctt atcaccacca ttgttggcgg
      121 ccttgtgctc gcctttatcc tcggcatgct ggccaataaa ctacgtattt ctcctctggt
      181 gggatatctg ttagcgggtg tgctggcagg accattcact ccgggctttg ttgccgatac
      241 caagcttgcg ccggaactgg ctgaactggg cgtcattctg ttgatgtttg gcgtgggtct
      301 gcacttttcg ctgaaggatt tgatggcggt aaaggccatc gccattcccg gtgcgatcgc
      361 ccagatagcc gtggcgacgc tgctgggtat ggcgctctct gccgtgctgg gctggtcgtt
      421 aatgaccggt atcgtgttcg gtttatgtct ttccaccgcc agtaccgtgg tgttactgcg
      481 cgcacttgaa gaacggcaat taattgacag tcagcgtggg caaatcgcca tcggttggtt
      541 gattgtggaa gacctggtaa tggttctgac gctggtgttg ctgcccgcag tggcaggaat
      601 gatggaacag ggcgatgtgg gctttgccac tcttgcagtc gatatgggga tcaccatcgg
      661 caaggtgatc gcatttatcg ccattatgat gctggtaggt cgccgtctgg tgccgtggat
      721 tatggcacgc agcgcggcaa ccggttctcg cgagctgttt accctgtcgg tgctggcgct
      781 ggcgttaggg attgcctttg gtgcggtgga gctgtttgat gtctcctttg cactcggtgc
      841 gttctttgcc gggatggtac tgaacgagtc tgaactgagt caccgtgccg cccacgatac
      901 gctgccattg cgcgacgcgt ttgcggtgct gttttttgtc tccgtcggga tgttgtttga
      961 tccgttaatt ctgattcagc aaccgctggc agtgctggcg acgctggcga ttattctgtt
     1021 tggtaagtcg ttagccgcat ttttcctggt gcgactgttt ggtcactccc aacgtacggc
     1081 attaactatc gccgccagcc tggcgcaaat tggtgagttc gcgtttatcc tggcgggact
     1141 gggcatggcg ctggatctgt tgccgcaagc cgggcagaac ctggtactgg caggtgcgat
     1201 tttgtcgatt atgctcaacc cggtcctgtt tgcgctgctg gagaaatatc tggcgaagac
     1261 cgaaacgctg gaagagcaga cgctggaaga ggcaatcgaa gaagagaagc agatcccagt
     1321 ggatatttgc aaccatgcgc tactggttgg ttacggtcgt gtaggcagcc tgctggggga
     1381 gaaattgctc gcctctgata ttccgctggt ggtgattgag acgtcacgaa cccgtgttga
     1441 tgagctgcga gagcgtgggg tccgcgctgt attgggcaat gcggcgaacg aagaaattat
     1501 gcaactggcg catctggaat gtgcaaaatg gctgatcctg acgattccca acggttatga
     1561 agcaggcgag attgtggcat ctgcccgcgc gaaaaatccg gatattgaga ttattgcccg
     1621 cgcccattat gacgatgaag tggcgtatat caccgaacgt ggcgcgaatc aggtagtgat
     1681 gggtgaacgt gaaattgccc gtactatgct ggaactgctg gaaacgccac cggcgggtga
     1741 agtggtgacg gggtaaacgg tggttgccgg ataataggcg cggatatgta ggcctgataa
     1801 gacgcgccag cgtcgcatca ggcattgaga gcaaaatgcc agatgcggcg tgaacgcctt
     1861 atctggccta cgaaccgacg ataacttaac gatcccagta agactcttcc aggctgtctt
     1921 cacgctccgg caagccgcgc gttaaacgag gtgaatgctg gttcagtacc tgatagctca
     1981 cacggttagc atatttacac acctgcgcta acgatgaata agttaaccag gtgaatttat
     2041 gtttgctgga gtttggtacg ttgctacggt ggtagctgtt ggcggtaatg tcatgcagca
     2101 acgctgccag tgcgccatcc cctgctccgt tggtgttcat gattttttcc ggcccgccca
     2161 tgtacggcgc aatgtgcgaa tagacgcgca gcggattctg gcaatccttg tggcgcatgg
     2221 cgcggctaaa ctcatactgg ttgaattccg ctatagcgcc cggcagcagc ggatgctggg
     2281 ttttacgttt cgcttcgtct tcggtaaagc ccgccatata caagccgatt ggcccggcgg
     2341 tgcacagcac cagatctacc cagtccagcg ccttgtcaga tgccaacaac ggatcgcttt
     2401 ctccggtcaa cgcttcggct tcatcttcgt tcatcgcaag gatagagacg tggtctttga
     2461 ggaattgctg ccaccactgt ggattctcgg caatgacaaa cttagttccc agcgtcagca
     2521 ccaccggtac gttatatttc ttcgcgtact caatggcttt catggttgct tccggcatgg
     2581 gttcacccgg cttgcaacgc accagataag aggtgagaac cagagccgag gctccggcaa
     2641 tcacatcttc cggaatactt tcagcccgca gctggttcat gtggccaggg ctgatagcaa
     2701 aggtacgttc cccggactcg ccaatcagcg taaagcaacg accaatcggg ccatccacgc
     2761 cttgtagata gttaagatcg gtacggctgg aggtgttaca caggtaacga taggcatagc
     2821 tgccaatttc aatattgctg cacatgacgc ccagcagcac cgaacggtcg tccgcgagca
     2881 ccgagtagtt atgcatggtg ttaccaatgg tgccacccgc aaactgatgg gtaatcaggt
     2941 ttttctgttt tagttcctga taaagcgctt cggctacatc atcctcaatc accagtgaat
     3001 gcccggcgct taatccataa cgctcaatga attcatcatc cactttcgct tcaatatcga
     3061 ccagcgtttg atcgataccc actacccagg cggcgctggt ttcgttttct ggctggaatt
     3121 gctgaagcag cggatcgcgt gcatttacgg ggaagtaatg tttggattta cgtttaccgg
     3181 gaaatttcat ggttgttttt acgggtggtt aactgagcgg cgaatgttaa cacaatctga
     3241 cgggagcgcg cgagacgcag ccagtttcaa tatcagcagg cgggatcgca ttttcatggc
     3301 agtggttcct tacaatgacg taatttgaaa ggagtttttg tatgaagccg gaaaacaaat
     3361 tacctgttct ggaccttatt tctgctgaaa tgaagatcgt tgtgaatact cttcagccgg
     3421 atttaccgcc ctggcccgca acgggaacga ttgctgagca acgacagtat tacacgcttg
     3481 agcgccgatt ctggaatgcg ggcgctccag aaatggcaac cagagcttac atggttccaa
     3541 caaaatatgg gcaggtggaa acacgtctct tttgtccgca gccagatagc ccggcaacgc
     3601 tattttattt gcatggaggc ggttttattc tcggcaatct cgatacccat gatcgcatca
     3661 tgcgcctgct ggcaagctac agccaatgta cggtgattgg cattgattac accctttcac
     3721 ctgaagcgcg ttttccgcaa gcgatagagg aaattgtggc tgcctgttgt tatttccacc
     3781 agcaggcgga ggattatcaa atcaatatgt cccgcattgg ctttgccggt gattccgcag
     3841 gtgccatgct ggcgctcgcc agtgcgttgt ggttgcgtga taaacagatc gattgcggta
     3901 aaattgcggg tgttttgctg tggtatgggc tttacggatt acgggattcc gtgactcgtc
     3961 gtctgttggg cggtgtctgg gatggcttaa cgcaacagga tttgcagatg tatgaagagg
     4021 cgtatttaag caactacgcg gaccgcgagt cgccgtatta ctgtctgttt aataatgatc
     4081 tcactcgcga agttccgccc tgttttattg ccggggcgga gttcgatccg ctgctggatg
     4141 acagccgtct gctttaccag acgttagcgg cgcatcagca gccctgtgag ttcaaactct
     4201 acccaggcac gctgcacgcc tttttgcatt attcacggat gatgaaaacc gccgacgagg
     4261 ctcttcgcga cggcgctcag ttctttaccg ctcagcttta gcgatacgcg gcaacaagat
     4321 tagccatcat ttcgatatgt tccggcgtgg cattaagcgc cggaatatat tcatattttt
     4381 tcccgccggc accgaggaag acctcacggt tttgctcggc aatctcttcc agcgtctcca
     4441 gacaatccgc agcaaagccc gggcacatca cctgtatatg acctacgcct ttttctccga
     4501 gcattttcag cgtttcgtcg gtataaggca tcagccaggg ttcccgacca aagcgcgact
     4561 gaaaggtcat catcactttt tccggtgcca tccccagtgc ggaagccagt tcgcgagtcg
     4621 ttgtgcggca acgttgcggg taatcatcgc cttcatctgc ataacgctgg ggaatgccat
     4681 gataagagag cagcagcaga tccggttcgc catgtttggc aaaagaagcg cgtacgctgt
     4741 tcgccagtgc attaatgtaa tcgtggttat cggcgtaatc acgtataaac gagatccccg
     4801 gaatgctacg tttgcgcgcc agaatgcgtg ccagttcatc ccataccgca ccgaccgtag
     4861 aacaggagaa ttgcggataa agcggcagca ccacaatatg atctacatgc tctgccagga
     4921 gttcatctac ggcgctttcc agtgatggcg agccgtagct cattcccagc gctacgggcg
     4981 tctccggtaa acgttgtgcc agcgcctgtt gttgctggcg gctgtaaacc atcagcggcg
     5041 agccaccttc catccagaca gaggcataca gcttcgccac acgcggcgag cgcagcggca
     5101 aaatcacgcc gcgcagcaat ggccaccata acaaccgtga ggtatcaacc acgcgtctgt
     5161 cgcttaaaaa ttgtttcaga tagcgtttta ccgcttcagg tgtgggggca tcgggcgtac
     5221 ccaggtttgc cagcaggata ccggttttag tctgacgcat taccgcctct tatcgattca
     5281 acttgttgat aattgtagcg gaaaagtgag gaagaagaac taattgctgt gagaggtatt
     5341 gcctgaggtg gatgagcaga cctgcccgaa agcagatctg ctgcggcgtg attagccgag
     5401 gattttttcc agatcagcgc gaacttcagc aaccggcttg gtgccgtcaa ctttcgcgta
     5461 tttggtatta cccgcttccg cttctttgga gtagtagccg atcagcggtg ctgtcatctg
     5521 atggtattca accagacgtt tacgtacggt ttcttcctga tcgtctttac gggtagtcag
     5581 ttcttcaccg gtaacgtcgt ctttaccttc tactttcggc ggattgaatt taacgtgata
     5641 aacacgacca gacggcgcgt gaacgcggcg accgacgata cggtcaacga tcagttcgtc
     5701 cggtacgtcg aattccagaa cgtaatcaac attgatgccc gcttctttca tcgcgtctgc
     5761 ctgcggaatg gtacgcggga agccgtccaa caggaaacca ttacggcagt cttcctgagc
     5821 aatgcgctct ttaaccagcg cgatcaccag ttcgtcggtg accagtttgc cagcatccat
     5881 aatgtctttt gcttgtttac ccagctcgga gccagatttg accgcagcac gcagcatatc
     5941 gccagtggag atttgcggaa taccatattt ctccatgatg aactgagcct gagtcccttt
     6001 ccccgcgccc ggagcgccaa gcagaatgat acgcattgcg aaaatcccct taaaatgtgt
     6061 cgaatttttt gaaaaagcga taaacgatac caccatcagg cgattgcctc aagaaaggca
     6121 ccaggggctg aaacggttaa tggcgagata atttgcggaa agtgcagata atgaaaagaa
     6181 aaatgccgga tgacacgaag gtcatccggc attacatcag gaaaccagca gctggttcat
     6241 acgacgaata aacaggttcg gatcttccag cgtgccgcgt tctgccagca gtgcctgatc
     6301 cagcagcagt tctacccact cgctgaactt cgcttcatct tcagtatctg ccgcacgttt
     6361 caccagtacg tgatccgggt tcagttcgaa gatgtatttc acttctggca ctttctggcc
     6421 cgccgcagcg aacagtttcg ccatctgagt gctcatttcg tccgcatcag tagagacaat
     6481 cgctggcgta tcggtcagac ggtgagtcag acggacatct ttcacgcgct cgccgagcag
     6541 ggctttcaca cggtcgatga acggagtcag tgctttctcc gcttctttcg cgctctcatc
     6601 aacttcgtca gccagttttt ccagcgactc gtcaacttta gacaccgact ggaacggttt
     6661 accgtcgaac tcagtcagat agttcatcat ccactcatcg atgcggtcgg aaagcagcag
     6721 aacttcgatg cctttcttac gcagcagttc caggtgcggg ctgctcttcg ctgccgcata
     6781 gctgtctgca gtgatgtagt agattttctc ctgcccttct ttcatgcggg aaacgtagtc
     6841 ttccagagac acggtctgcg cagaagaatc ggtatgagta gaagcaaaac gcagcagttt
     6901 ggcgatcgct tcctggttag cgaaatcttc cgccggacct tctttcagta ccaggccaaa
     6961 ctgttgccag aaggtctggt atttttccgc gtcgtctttc gccagttttt ccagcatttg
     7021 cagcacacgc ttggtcagcg cattgcgcag gttacgcgtt accgtgctgt cctggaggat
     7081 ttcacgggaa acgttcagcg gcagatcgct ggagtcaatc agaccacgca cgaagcgcag
     7141 atagttcggc atgaactgtt ctgcgtcgtc catgatgaac acacgctgaa catacagttt
     7201 caggccgtgt ttatgatcac ggttccacat atcccacgga gcctgggacg ggatgtacag
     7261 caggctggtg tactcctgct taccttcaac gcggttgtgg ctccaggtca gcggatcgtt
     7321 aaagtcgtgg gcgatgtgtt tgtagaactc tttgtactct tcatcggtga tttccgactt
     7381 gttacgagtc cacagcgcct gcgctttgtt aattttctcc caggagataa cggtttcgcc
     7441 gtctttctct tcgcgttttt cgatctctac cggcagcgcg atatggtcgg agtatttgct
     7501 gatgatggaa cgcacgcgcc agtcatcgag gaactcgtct tcgccttcac gcagatgcag
     7561 ggtgatttca gtaccacgat cttctttggt gatgtcggca acggtatatt caccttcgcc
     7621 agccgattcc cagaagacgc cgttttctgg tttttcgcct gccgcgcgag tacgcacggt
     7681 cactttgtcg gccacgataa acgcagagta gaaaccaaca ccaaactgac cgatcagctg
     7741 actgtctttc gcctggtcag aacccagcga ttcaaggaat gatttagtgc cggatttagc
     7801 gatagtcccc agatggtcaa tcacttcgtc gcgggtcatc cccacgccgt tatcggagat
     7861 ggtcagcgta cgcttgtctt tatcgaaaga gacacgaacg cgcagttcgc catcaccttc
     7921 gtacaggtcc gggttagaga gcgcgcggaa acgcagcttg tccgccgcat cggaggcgtt
     7981 agagataagc tcacgcagga agatttcttt attggaatag agagaatgga tcatcaggtg
     8041 cagaagctgt ttcacttctg actgaaaacc acgagtttct tgtcctttca tgtaggtcta
     8101 cctcaataat gccattttaa ggttaaaaac aggatgaggg agagatgggg acaagggaga
     8161 ataatttcaa gcgagagcag gtgatcctgc tctcgtttgc ttaaaaacga atcttatgac
     8221 gcccggcaag ggagtgtgac agcgtggtgc catcgaccat ttccagctcg ccgccaaccg
     8281 gtacgccatg agcgattcgg ctggcttcca cgtcatattg cgcgcaaagc tcggcaatgt
     8341 agttagcggt agcttcacct tcaaccgtag ggttggtggc gaggatcact tcagtgattt
     8401 tttcctctgc cagacgctgt tccagacgat caaggccgat atcatccgga ccgatgccgt
     8461 ccagcggtga cagatgtccc atcaacacaa aataacgacc tgaaaactgc cccgtctgct
     8521 caatggcgta aatgtccgcc ggactctcca ccacgcagat ttgaccgttt tcctgacgac
     8581 gcggattcga acagatgtta cagacttcct gttcggtgaa agtgcggcaa tcggcgcagt
     8641 ggccgatttc cgacatcgcc cgggtgagcg cctgcgccag acgcatcccg ccgctacgat
     8701 cgcgctgaag cagcgtgaac gccatacgct gcgccgactt cgggccaacg cccggcagac
     8761 agcgcagtgc ttccataagc tgtgttaaca gcgggctggt ttgcatcaga acggcatctt
     8821 aaagccaggc ggcagctgca ttccggagga tacagaggcc attttttctt tctgcgtttc
     8881 ttcaatacga cgtgctgcgt cgttgaatgc tgcagccacc aggtcttcca gcatctcttt
     8941 gtcgtcttcc agcaggctcg ggtcgatctc tacgcgacgg cagttgtgtg caccgttgat
     9001 ggtcactttt accagacctg cgccagattc gccggtgact tccagctgcg cgatctcttc
     9061 ctgcattttc tgcatttttt cttgcatctg ctgggcttgc ttcatcaggt tacccagacc
     9121 gcctttacca aacataggtt tctctctcaa tcacgttaag gatgacgaac gtaagctgtg
     9181 cttacgatca aatggggcgg atactttctt catccagctc cgcatcgaag aatcgacgca
     9241 gggtctgaat attattatcc gcaataatgg actcgcgcgc ctgcgcaagt ttttcttcgt
     9301 atatcgcctg acgccactcc agcggcgtac gcaccgcggg attatcatct tcaacgatag
     9361 tcagttcaac cgttgaacct tttaacatgc tcaacgcttc agccagtttt tgctgtgcac
     9421 cgcggttgtt caaatgccgc tgagaggagc gcaaatgcag acatactgcg ttatcactct
     9481 cctctttcca ggcatttaag gccacctgtt cgaccagttt tggtagcgaa agttgactca
     9541 cctgagccgc ccacgcgtcg cgctcaatgg cttctgccgc cagcttcgcc gccagttccg
     9601 gcgttttttc atgttccagc gcttttttca gcgccttcgg tgtggcgacc acttcttttt
     9661 gctgcatcac tggagtggtc gccttccagc gatacgcttc ttttttagct ggcgcttttt
     9721 ccagcgccga tggcaccgga cgcgcctgaa cgcgatccgt gaccgaagcc agtctttcca
     9781 gcgcagcgtt attcaccggc cgcgcgcggg tagcggctgc cggttcactc ttttttgctt
     9841 tggttgctcc ctgcacgcgc tgcaactgct ggcgcgccgc cagcacctgg ctggtggttt
     9901 ccgggagcgg tacagtcggt gcctgctgcg gcgctgattg cggttgcggc ggcacctggg
     9961 ttggcgtcat tactgccgtt ggcgcgacgg gtgcaaagga ctggcgtggc acttctggct
    10021 caggcagcgg catacgcgga tggaacgcca gcgcgcgcag cagcgtcatc tcaacgccca
    10081 tgcggcggtc cggcgcatac ggtaattctt tgcgaccaat caacagcgtc tgatagtaaa
    10141 gctgaatatc cgtcggcggt atggtgcgcg ccagttcacg catccgcagc tcgatggcgg
    10201 ccatgtcgtt gccaagtgca gcaggcgaaa gttgtaccat cgcaatacga tgcaacaggc
    10261 cgagcatttc caccagcaac gcttcccact cgataccacg ggcagcggct tcattaatca
    10321 gcgccattac gcgctcgccg ttggcctcga ccatcgcttc aaccagcgac agcgcctgat
    10381 cgtcgtcaag cgtacccagc atcgcactga ccgcctgggt tgaaacctgg ccgtcaccgc
    10441 tggcaatcgc ctggtcggtc agacttaagg catctcgcag gctgccttca gcggcgcgcg
    10501 ccagcaattg cagcgcccgc ggctcgtgag cgatatgttc ttcattgagg atgtgctcaa
    10561 gttgatggcg aatttgctcg acatccagcg ccttgagatg aaattgcaaa cagcgcgaca
    10621 aaatcgtcac cggcaatttc tgtgggtcgg tcgtcgccag cagaaactta acgtgctccg
    10681 gcggctcttc aagagttttt aacagtgcgt taaagctgtg gcgcgacagc atatgcactt
    10741 cgtcgatgag ataaactttg aaacgaccac gcgccggagc gtactggacg ttatccagca
    10801 ggtcgcgagt gtcttcaact ttggtgcgcg aggcggcgtc gatttcaatc agatcgacaa
    10861 agcgcccctg ctcgatttca cgacagttat cgcacacgcc gcacggcgtc gcggtaatgc
    10921 cggtttcgca gtttagcccc ttcgccagca gtcgggcgat agaggttttt ccgacgccac
    10981 gggtaccgga aaaaagataa gcatgatgaa tacgccctaa cgacaagccg ttcgccagtg
    11041 cggtcagcac atgttcctgg ccgacgacgt cagcaaaggt ttgtgggcgc cattttcggg
    11101 ctaagacctg ataactcatt ggcaggctct gaaacgctgg aaggtggatt cacgaagggg
    11161 taatgctaac acagccccgt cagaacggcg aggctgaatg cacatcttgt gctgtccgta
    11221 gcgtgggcag cacaagactg gcgataatta atggcccggg aacgggacaa ggctgtagct
    11281 ggtaatgccc tgtttttcga gacgctgttc gccgccgaga tcgaacaggt tgataatgaa
    11341 cgcagcgtca gccacttcac cacccagacg acggatcagt ttaacggtcg cttcgatagt
    11401 gccgccggtt gccagcagat cgtccaccac cagaactttg tcgcccggtt tgatggcatc
    11461 aacgtggatc tccagctgat cggtgccgta ttccagatcg taagtttcac tgatagtttc
    11521 acgcggcagt ttgcccggtt tacggaccgg tacaaagcca acgcccagac ccagagctac
    11581 cggagcgcca aacaagaagc cacgcgcttc ggtgccgaca actttggtaa tgcccgcatt
    11641 tttgtaacgc tcaaccagca agtcgatgct gagagcgtaa gctttcgggt cttccagtaa
    11701 gctggtgaca tcgcggaaaa gaatgccggg ttttgggtag tcctgaatgc ttttgatgct
    11761 atttttgaga tactcaagct gctgtgcagt cgcggtcata agtgtatgcc tgcttgttac
    11821 ggtggtactc acggcgcgtt ttaaacgtat caaaagtaac agttgtttaa cctttgaccg
    11881 cctgcgcctg tgctcgaaaa cgccagaatg tactggctgt acgcaataat tgcaactgcg
    11941 attgttgtgc ttcagtgctt ttcttgcttt tcatcaatca ccggaattcg ccacatatag
    12001 aaaagcaaac aggcgagaat taccagcaac atgatgcgca cccatggcat ctggacgaac
    12061 cacagagaaa tggcaaacgt gagcaaaata agcaaaatcg cccgcggttt gacgccgcgc
    12121 ggcatcgcat gatgtttctg ccagaaacgt agatagctgc caaaccatga gcggtacagc
    12181 aaccaggcgt gaaagcgcgg ggaagaacgg gcaaagcacc aggccgctag caggataaac
    12241 ggcgtcgtcg gtaataccgg taataccacg cccagcgtac ccagcactac cgccagccag
    12301 ccaatgatga ttaaaatgat tcgttgcata ttgagtgttg tcagcttaca gagtggctac
    12361 tttagcataa caattatcat tttcattgag gtcttatcgt gaaaaccgcc ctgctgctgg
    12421 aaaaactgga aggacagctc gccacgctgc gccagcgttg tgccccggta gcgcaatttg
    12481 ccacgctaag tgcccgcttt gacaggcacc tttttcagac ccgtgcgaca acactgcagg
    12541 cttgtctcga tgaggcgggc gataatctgg ctgcgcttcg tcatgcagtt gagcaacagc
    12601 aactgccgca agtcgcctgg ctggcagaac atctggcggc gcaactggag gctatagcgc
    12661 gtgaagccac agcctggtca ttgcgcgagt gggacagtgc gccaccgaaa attgcccgct
    12721 ggcagcgtaa acgtattcag catcaggatt ttgagcggcg gctacgtgag atggttgccg
    12781 aacgtagagc ccgcctggcg cgggtgaccg atctcgtgga acagcaaacg ctgcatcgtg
    12841 aagtggaagc ctatgaagcg cgcctggcgc ggtgtcgcca tgcgctggaa aaaatcgaaa
    12901 acaggttagc gcgtttaacc cgctagcaat ggagagagta tgtcactgga aaatgccccc
    12961 gacgatgtca aactggccgt cgatttgatt gtgctgctgg aagaaaatca gatcccagcc
    13021 cgcaccgtgc tgcgcgcgct ggatattgta aaacgcgatt atgaaaagaa attaacgcgc
    13081 gatgatgagg cggaaaagtg agaaataaat ggggtggcta aatgtcccct aaggttgcgg
    13141 gcaaacgcaa aactgcctga tgcgctacgc ttatcaggcc tacaccgatc ctgcaatata
    13201 ttgaattttt gcggtttcgt aggccggata aggcggtcac gccgcatccg gcatgaacaa
    13261 cgcgcacttt gttagcaatc tcggggcaac aaaatgtcgc cccctttcgt tattacccta
    13321 ccgctggcgt cgggtcatcg cctttgtagt cgcgttttac ttccgtcacc tcatcgccct
    13381 tctcgttgtg cagatgcact tcaagctggt taaaggcaat gttgatgtcg ttttcacggc
    13441 acagctgatc gatagtacgg ttcagctcat cgacagtacg gctacggtca cgcagttcac
    13501 gcacatacag acgcagctca tgatccaacg tgctggcacc aaacgccgta aagaagactt
    13561 ccggcattgg ttcgtgcatc acccttgggt gctcagtcgc cgccttcagt aacactttac
    13621 gcactttttc cagatcggag ccataggcca cgccgagacg gatcaccaga cgcgtagtag
    13681 tgtcagtcaa cgaccagttg atcagacgct cggtaacaaa cgctttgttc gggatgatca
    13741 cttctttgcg atcgaaatcg gtaatcgttg tcgcacgaat acggatctta cttaccgtcc
    13801 ccgagaagct accaatggtt accgtatcgc caatacgcac cggacgttcg aatagaatga
    13861 tcaaaccgga gacgaagtta ccgaaaattt cttgtaaacc aaaaccgaga cctacggata
    13921 atgctgctgc cagccactgg agtttatccc aagagacgcc cagcgatccg aacaccgtca
    13981 tcgcaccaac agcaataatg atgtagttaa ggatggtagt aatggcatac gacgcgccct
    14041 ggcgcatatt cagtcgcgag agcaccagca cttccagcaa accaggcagg ttgcgaatca
    14101 acgcccaggc caccattgag gcgataatcg caaacaacag actgcccatg gtgacgtttt
    14161 tcaccaccgc agcaccagct tcagtaccgt tgtaatgcca cagcgtaatg ctgtcgagat
    14221 agctgaacac ggtgatcaaa tcggaccaaa ttgcccagaa catgacaccg aacagcgcaa
    14281 acatcagcaa catggtaata cgcagcgtct gctggttaac ctgttccagt gcaatggtgg
    14341 gttcttccgg cggttcggca ccttctgcgc cctctttcac cagattctgc cgacgcgcca
    14401 gcgcacgacg ccaggcgata cgccgcgccg ctacgcttaa gccgcgcaat accgtctggt
    14461 agagcaagtt ccagatgatc accagataaa cggtttcaat ccaacgcccg gagaggcgca
    14521 gcgtggtgta gaaataaccc gtggcagtca gcaccatcaa tgcaatcggg ataatcgaca
    14581 gcacggtaat ggtgaccagt cgcatggtgt gcgactcttt atcacgccag ctttcgcggc
    14641 acattggcca taccaggaag gcaatcagca gcaggttgaa gaaaatcatc gcctgcccca
    14701 gcacatcatc catcagatgc agcggggaaa gttctgccac cacagaccag aaatggatag
    14761 gcagcaacgc gagactgatg cggacaattt gccgacgcca gtggctggtc tgctgctccg
    14821 gcatgccgaa gtgacgcacg gcaacgccat ttttctccag taccttccag cacaggccaa
    14881 acaccagcca gaaaatcgcc agttttttac tgaacgacca tagcagttcg ctgatgttga
    14941 gctgcatggt caacagaatc aggccaaccg cgagaataat caggcacacc ggcagcgcac
    15001 ggatcaggtc gataaggatc gcttttggtg tgttgagctg gctgtcgtta cgcagggaac
    15061 ccacagcgga agccagtttt tgttgatacg ctttcagcca ccccagacgc cagtggatca
    15121 gcccggcaat caacagcagc ggcaaaccag cgaggaaagc gataaaaacg gcgggccagg
    15181 ctttttccca gttcacctta attttcatcg acttaaattc atctttcagg ctttgcggga
    15241 acgctttgat ccagtcccag tccattggac ggttactgtt cacccaaaag atttgctgag
    15301 tcaggatgga tttcaggttt ttcgacacac tcattaactg ctgctggttg atttgcaggt
    15361 taatggccat catcagctgg ttacccaact gtttgttgag ttgatccagc aattcgcgac
    15421 gcatatcaac cacttgcaat aacgcatcgt gaacttcgct gttgacttcg ttggtgtgac
    15481 cttcttccag tttgttgacg aacgcatcgc tctggaagag tgcatcacgc tgctggttaa
    15541 cttcaaactg ttcgagacgc aaatccgcga tgcggttggt catgttttcc agttcatccg
    15601 ccgagggcag cgtttgttgt tgctggtaaa ggatacgaga caacagcagg ctgcccttca
    15661 ggacggcaat ctgctcttta atattgcgtt ccgattgcag cgcccgctcc agccagtttt
    15721 tgactttaat gttttgctgc atcaactgat taccgttttc agtcgcggta atcagacgct
    15781 gacttaactg ctggttaatt tccagttcct gcttcaccag cggattagcc tgaatacgcg
    15841 cggcttcatc cggggagacg gcttcctgcg ccgttttttc ggttaaagtc aggcgcttgc
    15901 tgtttaccgc ttcttgcaac agttgtaact ggtgctccag acgggcgctg ttcgccgtca
    15961 cgtaatcacg ttgcttttgc aaggtatcct gcaagacggt attcccttcc aggcttttac
    16021 gctgctggtc aatctccgca ttcagcaacg cctgctgagc ctgcattaac actttctggc
    16081 tgggacgtaa ggcagtctcg ccgacatcag tcccatccag acgactgcga atttgttgca
    16141 gctgctgcga agcgttatac atcgcatttt gcacgcgttc gggctgcgtc tgtaacgaaa
    16201 ccagctggct gttataagac gctagatcgt tttgtgcgtt ttgcaaatcg tccagcgcct
    16261 gggcaacgcg agtttccagc tggcgcaacg aaagcgtgct cagaattttg cgcgtttctt
    16321 cgtcgttatc gacatcgcta agtgctgtta acgccgcggt cgcctggcgc attttttccg
    16381 gcgcttcagc gactttttgc cgtagctgaa cggtctcttc ttttacgcga tcgattttat
    16441 cgagggtggc taatgtatct gtcagatcct gctgcaccag tttgtcctga gcagaaagat
    16501 ctttttgttt atttagtgag tcaagttgcg cctgcaggtc cgcttttgtc ggcagatcac
    16561 cattcgatga cgcccgcgca aacgccgtgt tctggcataa caagacaaaa acaaaagcaa
    16621 taaatgctga aaaaacaaaa tgccgtgatc gtttgtaata ctggaacata gtcatgatga
    16681 atgaaggttt ctgaacctga agaacgacct gaaaaagtca aaccgcaaga atatcacgac
    16741 gcagtgaacc agaatagcaa cgacgaaaat gtccaggaaa aatcctggag tcagattcag
    16801 ggttattcgt tagtggcagg gttacgaagc gtggggcaca ggagatacat ctccagtaag
    16861 atggcgacgt aatcgcgggc ttctttttta agatcaaaag attgcggggc aaagagccag
    16921 ttttccatca ggccggaaat atagccgcgc ataataattg ctgcgcgacg cgtcattaaa
    16981 tccgcaggca acattttcgc ttcaatacaa tgttttaacg tttgttctat acggtcataa
    17041 ctttccagac agagattacg ttgtgcctgt tgcacaacag ccatttctcc gacaaattcg
    17101 catttgtgga atataatctc catcaataat cgacgccgtt cttctgtcac cgtggattca
    17161 agaacatgaa ttaatatctc tcttaatact gagagtggat cgccagggaa ttttgcctga
    17221 tactcaagct ctagttcacc aatattggat tctgacagtt cccagatctc actgaacaaa
    17281 tccgacttgt ctttaaaatg ccagtagatt gcaccgcgcg taacgccagc tgcttttgca
    17341 atctcgccca gcgaggtgga tgataccccc tgctgtgaga aaagacgtag agccacatcg
    17401 aggatgtgtt ggcgcgtttc ttgcgcttct tgtttggttt ttcgtgccat atgttcgtga
    17461 atttacaggc gttagattta catacatttg tgaatgtatg taccatagca cgacgataat
    17521 ataaacgcag caatgggttt attaactttt gaccattgac caatttgaaa tcggacactc
    17581 gaggtttaca tatgaacaaa aacagagggt ttacgcctct ggcggtcgtt ctgatgctct
    17641 caggcagctt agccctaaca ggatgtgacg acaaacaggc ccaacaaggt ggccagcaga
    17701 tgcccgccgt tggcgtagta acagtcaaaa ctgaacctct gcagatcaca accgagcttc
    17761 cgggtcgcac cagtgcctac cggatcgcag aagttcgtcc tcaagttagc gggattatcc
    17821 tgaagcgtaa tttcaaagaa ggtagcgaca tcgaagcagg tgtctctctc tatcagattg
    17881 atcctgcgac ctatcaggcg acatacgaca gtgcgaaagg tgatctggcg aaagcccagg
    17941 ctgcagccaa tatcgcgcaa ttgacggtga atcgttatca gaaactgctc ggtactcagt
    18001 acatcagtaa gcaagagtac gatcaggctc tggctgatgc gcaacaggcg aatgctgcgg
    18061 taactgcagc gaaagctgcc gttgaaactg cgcggatcaa tctggcttac accaaagtca
    18121 cctctccgat tagcggtcgc attggtaagt cgaacgtgac ggaaggcgca ttggtacaga
    18181 acggtcaggc gactgcgctg gcaaccgtgc agcaacttga tccgatctac gttgatgtga
    18241 cccagtccag caacgacttc ctgcgcctga aacaggaact ggcgaatggc acgctgaaac
    18301 aagagaacgg caaagccaaa gtgtcgctga tcaccagtga cggcattaag ttcccgcagg
    18361 acggtacgct ggaattctct gacgttaccg ttgatcagac cactgggtct atcaccctac
    18421 gcgctatctt cccgaacccg gatcacactc tgctgccggg tatgttcgtg cgcgcacgtc
    18481 tggaagaagg gcttaatcca aacgctattt tagtcccgca acagggcgta acccgtacgc
    18541 cgcgtggcga tgccaccgta ctggtagttg gcgcggatga caaagtggaa acccgtccga
    18601 tcgttgcaag ccaggctatt ggcgataagt ggctggtgac agaaggtctg aaagcaggcg
    18661 atcgcgtagt aataagtggg ctgcagaaag tgcgtcctgg tgtccaggta aaagcacaag
    18721 aagttaccgc tgataataac cagcaagccg caagcggtgc tcagcctgaa cagtccaagt
    18781 cttaacttaa acaggagccg ttaagacatg cctaatttct ttatcgatcg cccgattttt
    18841 gcgtgggtga tcgccattat catcatgttg gcaggggggc tggcgatcct caaactgccg
    18901 gtggcgcaat atcctacgat tgcaccgccg gcagtaacga tctccgcctc ctaccccggc
    18961 gctgatgcga aaacagtgca ggacacggtg acacaggtta tcgaacagaa tatgaacggt
    19021 atcgataacc tgatgtacat gtcctctaac agtgactcca cgggtaccgt gcagatcacc
    19081 ctgacctttg agtctggtac tgatgcggat atcgcgcagg ttcaggtgca gaacaaactg
    19141 cagctggcga tgccgttgct gccgcaagaa gttcagcagc aaggggtgag cgttgagaaa
    19201 tcatccagca gcttcctgat ggttgtcggc gttatcaaca ccgatggcac catgacgcag
    19261 gaggatatct ccgactatgt ggcggcgaat atgaaagatg ccatcagccg tacgtcgggc
    19321 gtgggtgacg ttcagttgtt cggttcacag tacgcgatgc gtatctggat gaacccgaat
    19381 gagctgaaca aattccagct aacgccggtt gatgtcatta ccgccatcaa agcgcagaac
    19441 gcccaggttg cggcgggtca gctcggtggt acgccgccgg tgaaaggcca acagcttaac
    19501 gcctctatta ttgctcagac gcgtctgacc tctactgaag agttcggcaa aatcctgctg
    19561 aaagtgaatc aggatggttc ccgcgtgctg ctgcgtgatg tggcgaagat tgagctgggt
    19621 ggtgagaact acgacatcat cgcagagttt aacggccaac cggcttccgg tctggggatc
    19681 aagctggcga ccggtgcaaa cgcgctggat accgctgcgg caatccgtgc tgaactggcg
    19741 aagatggaac cgttcttccc gtcgggtctg aaaattgttt acccgtacga caccacgccg
    19801 ttcgtgaaaa tctctattca cgaagtggtt aaaacgctgg tcgaagcgat catcctcgtg
    19861 ttcctggtta tgtatctgtt cctgcagaac ttccgcgcga cgttgattcc gaccattgcc
    19921 gtaccggtgg tattgctcgg gacctttgcc gtccttgccg cctttggctt ctcgataaac
    19981 acgctaacaa tgttcgggat ggtgctcgcc atcggcctgt tggtggatga cgctatcgtt
    20041 gtggtagaaa acgttgagcg tgtgatggcg gaagaaggtt tgccgccaaa agaagccacc
    20101 cgtaagtcga tggggcagat tcagggcgct ctggtcggta tcgcgatggt actgtcggcg
    20161 gtattcgtac cgatggcctt ctttggcggt tctactggtg ctatctatcg tcagttctct
    20221 attaccattg tttcagcaat ggcgctgtcg gtactggtag cgttgatcct gactccggct
    20281 ctttgtgcca ccatgctgaa accgattgcc aaaggcgatc acggggaagg taaaaaaggc
    20341 ttcttcggct ggtttaaccg catgttcgag aagagcacgc accactacac cgacagcgta
    20401 ggcggtattc tgcgcagtac agggcgttac ctggtgctgt atctgatcat cgtggtcggc
    20461 atggcctatc tgttcgtgcg tctgccaagc tccttcttgc cagatgagga ccagggcgta
    20521 tttatgacca tggttcagct gccagcaggt gcaacgcagg aacgtacaca gaaagtgctc
    20581 aatgaggtaa cgcattacta tctgaccaaa gaaaagaaca acgttgagtc ggtgttcgcc
    20641 gttaacggct tcggctttgc gggacgtggt cagaataccg gtattgcgtt cgtttccttg
    20701 aaggactggg ccgatcgtcc gggcgaagaa aacaaagttg aagcgattac catgcgtgca
    20761 acacgcgctt tctcgcaaat caaagatgcg atggttttcg cctttaacct gcccgcgatc
    20821 gtggaactgg gtaccgcaac cggctttgac tttgagctga ttgaccaggc tggccttggt
    20881 cacgaaaaac tgactcaggc gcgtaaccag ttgcttgcag aagcagcgaa gcaccctgat
    20941 atgttgacca gcgtacgtcc aaacggtctg gaagataccc cgcagtttaa gattgatatc
    21001 gaccaggaaa aagcgcaggc gctgggtgtt tctatcaacg acattaacac cactctgggc
    21061 gctgcatggg gcggcagcta tgtgaacgac tttatcgacc gcggtcgtgt gaagaaagtt
    21121 tacgtcatgt cagaagcgaa ataccgtatg ctgccggatg atatcggcga ctggtatgtt
    21181 cgtgctgctg atggtcagat ggtgccattc tcggcgttct cctcttctcg ttgggagtac
    21241 ggttcgccgc gtctggaacg ttacaacggc ctgccatcca tggaaatctt aggccaggcg
    21301 gcaccgggta aaagtaccgg tgaagcaatg gagctgatgg aacaactggc gagcaaactg
    21361 cctaccggtg ttggctatga ctggacgggg atgtcctatc aggaacgtct ctccggcaac
    21421 caggcacctt cactgtacgc gatttcgttg attgtcgtgt tcctgtgtct ggcggcgctg
    21481 tacgagagct ggtcgattcc gttctccgtt atgctggtcg ttccgctggg ggttatcggt
    21541 gcgttgctgg ctgccacctt ccgtgggctg accaatgacg tttacttcca ggtaggcctg
    21601 ctcacaacca ttgggttgtc ggcgaagaac gcgatactta tcgtcgaatt cgccaaagac
    21661 ttgatggata aagaaggtaa aggtctgatt gaagcgacgc ttgatgcggt gcggatgcgt
    21721 ttacgtccga tcctgatgac ctcgctggcg tttatcctcg gcgttatgcc gctggttatc
    21781 agtactggtg ctggttccgg cgcgcagaac gcagtaggta ccggtgtaat gggcgggatg
    21841 gtgaccgcaa cggtactggc aatcttcttc gttccggtat tctttgtggt ggttcgccgc
    21901 cgctttagcc gcaagaatga agatatcgag cacagccata ctgtcgatca tcattgatac
    21961 aacgtgtaat cactaaggcc gcgtaagcgg ccttttttat gcataaccta cgaacattaa
    22021 ggagtaattg aaccaccaac tcaggatctc atacgaaaac cagtattaac cacggataaa
    22081 attcataaa
//
LOCUS       DAZKFU020000060        21944 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZKFU020000060 DAZKFU020000000
VERSION     DAZKFU020000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.060"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            7..2268
                     /gene="metE"
                     /locus_tag="ACHOQQ_RS08975"
                     /old_locus_tag="pgaptmp_001795"
     CDS             7..2268
                     /gene="metE"
                     /locus_tag="ACHOQQ_RS08975"
                     /old_locus_tag="pgaptmp_001795"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGU4470059.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAQRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(2308..3123)
                     /gene="ysgA"
                     /locus_tag="ACHOQQ_RS08980"
                     /old_locus_tag="pgaptmp_001796"
     CDS             complement(2308..3123)
                     /gene="ysgA"
                     /locus_tag="ACHOQQ_RS08980"
                     /old_locus_tag="pgaptmp_001796"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGU4470060.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGSQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            3385..4146
                     /gene="udp"
                     /locus_tag="ACHOQQ_RS08985"
                     /old_locus_tag="pgaptmp_001797"
     CDS             3385..4146
                     /gene="udp"
                     /locus_tag="ACHOQQ_RS08985"
                     /old_locus_tag="pgaptmp_001797"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGU4470061.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            4287..5714
                     /gene="rmuC"
                     /locus_tag="ACHOQQ_RS08990"
                     /old_locus_tag="pgaptmp_001798"
     CDS             4287..5714
                     /gene="rmuC"
                     /locus_tag="ACHOQQ_RS08990"
                     /old_locus_tag="pgaptmp_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGU4470062.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINSDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            5809..6564
                     /gene="ubiE"
                     /locus_tag="ACHOQQ_RS08995"
                     /old_locus_tag="pgaptmp_001799"
     CDS             5809..6564
                     /gene="ubiE"
                     /locus_tag="ACHOQQ_RS08995"
                     /old_locus_tag="pgaptmp_001799"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGU4470063.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            6578..7183
                     /gene="ubiJ"
                     /locus_tag="ACHOQQ_RS09000"
                     /old_locus_tag="pgaptmp_001800"
     CDS             6578..7183
                     /gene="ubiJ"
                     /locus_tag="ACHOQQ_RS09000"
                     /old_locus_tag="pgaptmp_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGU4470064.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYIGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            7180..8820
                     /gene="ubiB"
                     /locus_tag="ACHOQQ_RS09005"
                     /old_locus_tag="pgaptmp_001801"
     CDS             7180..8820
                     /gene="ubiB"
                     /locus_tag="ACHOQQ_RS09005"
                     /old_locus_tag="pgaptmp_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGU4470065.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            8899..9168
                     /gene="tatA"
                     /locus_tag="ACHOQQ_RS09010"
                     /old_locus_tag="pgaptmp_001802"
     CDS             8899..9168
                     /gene="tatA"
                     /locus_tag="ACHOQQ_RS09010"
                     /old_locus_tag="pgaptmp_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGU4470066.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            9172..9687
                     /gene="tatB"
                     /locus_tag="ACHOQQ_RS09015"
                     /old_locus_tag="pgaptmp_001803"
     CDS             9172..9687
                     /gene="tatB"
                     /locus_tag="ACHOQQ_RS09015"
                     /old_locus_tag="pgaptmp_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGU4470067.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            9690..10466
                     /gene="tatC"
                     /locus_tag="ACHOQQ_RS09020"
                     /old_locus_tag="pgaptmp_001804"
     CDS             9690..10466
                     /gene="tatC"
                     /locus_tag="ACHOQQ_RS09020"
                     /old_locus_tag="pgaptmp_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGU4470068.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            10508..11290
                     /gene="tatD"
                     /locus_tag="ACHOQQ_RS09025"
                     /old_locus_tag="pgaptmp_001805"
     CDS             10508..11290
                     /gene="tatD"
                     /locus_tag="ACHOQQ_RS09025"
                     /old_locus_tag="pgaptmp_001805"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGU4470069.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11287..11775)
                     /gene="rfaH"
                     /locus_tag="ACHOQQ_RS09030"
                     /old_locus_tag="pgaptmp_001806"
     CDS             complement(11287..11775)
                     /gene="rfaH"
                     /locus_tag="ACHOQQ_RS09030"
                     /old_locus_tag="pgaptmp_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGU4470070.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            11942..13435
                     /gene="ubiD"
                     /locus_tag="ACHOQQ_RS09035"
                     /old_locus_tag="pgaptmp_001807"
     CDS             11942..13435
                     /gene="ubiD"
                     /locus_tag="ACHOQQ_RS09035"
                     /old_locus_tag="pgaptmp_001807"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGU4470071.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            13481..14182
                     /gene="fre"
                     /locus_tag="ACHOQQ_RS09040"
                     /old_locus_tag="pgaptmp_001808"
     CDS             13481..14182
                     /gene="fre"
                     /locus_tag="ACHOQQ_RS09040"
                     /old_locus_tag="pgaptmp_001808"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGU4470072.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(14374..15537)
                     /gene="fadA"
                     /locus_tag="ACHOQQ_RS09045"
                     /old_locus_tag="pgaptmp_001809"
     CDS             complement(14374..15537)
                     /gene="fadA"
                     /locus_tag="ACHOQQ_RS09045"
                     /old_locus_tag="pgaptmp_001809"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGU4470073.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(15547..17736)
                     /gene="fadB"
                     /locus_tag="ACHOQQ_RS09050"
                     /old_locus_tag="pgaptmp_001810"
     CDS             complement(15547..17736)
                     /gene="fadB"
                     /locus_tag="ACHOQQ_RS09050"
                     /old_locus_tag="pgaptmp_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGU4470074.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLVEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            17926..19257
                     /gene="pepQ"
                     /locus_tag="ACHOQQ_RS09055"
                     /old_locus_tag="pgaptmp_001811"
     CDS             17926..19257
                     /gene="pepQ"
                     /locus_tag="ACHOQQ_RS09055"
                     /old_locus_tag="pgaptmp_001811"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGU4470075.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            19257..19871
                     /gene="yigZ"
                     /locus_tag="ACHOQQ_RS09060"
                     /old_locus_tag="pgaptmp_001812"
     CDS             19257..19871
                     /gene="yigZ"
                     /locus_tag="ACHOQQ_RS09060"
                     /old_locus_tag="pgaptmp_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGU4470076.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            19910..21361
                     /gene="trkH"
                     /locus_tag="ACHOQQ_RS09065"
                     /old_locus_tag="pgaptmp_001813"
     CDS             19910..21361
                     /gene="trkH"
                     /locus_tag="ACHOQQ_RS09065"
                     /old_locus_tag="pgaptmp_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGU4470077.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFRQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            21373..21918
                     /gene="hemG"
                     /locus_tag="ACHOQQ_RS09070"
                     /old_locus_tag="pgaptmp_001814"
     CDS             21373..21918
                     /gene="hemG"
                     /locus_tag="ACHOQQ_RS09070"
                     /old_locus_tag="pgaptmp_001814"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGU4470078.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         5054 a         5596 c         6096 g         5198 t
ORIGIN      
        1 agaaaaatga caatattgaa tcacaccctc ggtttccctc gcgttggcct gcgtcgcgag
       61 ctgaaaaaag cgcaagagag ttattgggcg gggaactcca cgcgtgaaga actgctggcg
      121 gtagggcgtg aattgcgtgc tcgtcactgg gatcaacaaa agcaagcggg tatcgacctg
      181 ctgccggtgg gcgattttgc ctggtacgat catgtactga ccaccagtct gctgctgggt
      241 aacgttccgg cgcgtcatca gaacaaagat ggttcggtag atatcgacac cctgttccgt
      301 attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg cagcggaaat gaccaaatgg
      361 tttaacacca actatcacta catggtgccg gagttcgtta aaggccaaca gttcaaactg
      421 acctggacgc agctgctgga cgaagtggac gaggcgctgg cgctgggcca caaggtgaaa
      481 cctgtgctgc tggggccgat tacctggctg tggctgggga aagtgaaagg tgaacagttt
      541 gaccgcctta gcctgctgaa cgacattctg ccggtttatc agcaagtgct ggcagaactg
      601 gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg cgctggtact ggaactacca
      661 caggcgtggc tggacgcata caaacccgct tacgacgcgc tccagggaca ggtgaaactg
      721 ctgctgacca cctattttga aggcgtaacg ccaaatctcg acacgattac tgcgctgcct
      781 gttcaggggc tgcatgtgga ccttgtacat ggtaaagatg acgttgttga actgcacaag
      841 cgcctgcctt ctgactggct gctgtctgcg ggtctgatca atggtcgtaa cgtctggcgc
      901 gccgatctta ctgagaaata tgcgcaaatt aaggacattg tcggcaaacg tgatttgtgg
      961 gtggcatcat cctgctcgtt gctgcacagc cccatcgacc tgagcgtgga aacgcgtctt
     1021 gatgcagaag tgaaaagctg gtttgccttc gccctacaaa aatgccatga actggcactg
     1081 ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg cagagtggag cgccccaatt
     1141 caggcgcgtc gtcactctac tcgtgtacat aacccggcag tagaaaagcg tctggcggcg
     1201 atcaccgccc aggacagcca gcgtgcgaat gtctatgaag tgcgtgccga agcccagcgt
     1261 gcgcgtttta aactgccagc gtggccgacc accacgattg gttccttccc gcaaaccacg
     1321 gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc tcgacgccaa taactaccgc
     1381 acgggcattg cggaacatat caggcaggcc attgttgaac aggaacgttt gggactggat
     1441 gtgctggtac atggcgaggc cgagcgtaat gacatggtgg aatattttgg cgagcacctc
     1501 gacgggtttg tctttacgca aaacggttgg gtacagagct atggttcccg ctgcgtgaag
     1561 ccaccgattg tcattggtga tattagccgc ccggcaccga ttaccgtgga gtgggcgaag
     1621 tatgcgcaat ctctgactga taaaccggtg aaagggatgc tgactggccc ggtgaccatt
     1681 ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa ccatcgccaa acaaattgcg
     1741 ctggcgctgc gtgatgaagt ggcggacctg gaagccgcag gaattggcat catccagatt
     1801 gatgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta gcgactggga tgcatatctc
     1861 cagtggggcg tggaggcctt ccgtatcaac gccgccgtgg cgaaagatga cacacaaatc
     1921 cacactcaca tgtgttattg cgagttcaac gacatcatgg attcgattgc ggcactggat
     1981 gcagacgtca tcaccatcga aacctcgcgt tccgacatgg agttgctgga gtcgtttgaa
     2041 gagtttgatt atccgaatga aatcggtcca ggcgtctatg acattcactc gccaaacgta
     2101 ccgagcgtag aatggattga agccttgctg aagaaagcag cacaacgcat tccggcagag
     2161 cgcctgtggg ttaacccgga ctgtggtctg aaaacgcgcg gctggccaga aacccgcgcc
     2221 gcgctggcga acatggtaca ggcggcgcag aatttgcgtc gggggtaaaa tccaaaccgg
     2281 gtggtaatac cacccggtct tttctcatta cagcgacttc ttcccaccat actgcttaaa
     2341 ccattccagc atacgctgcc agccatcttt cgcagattcg gcatgatagc tcgggcgata
     2401 atcagcgttg aatgcatgcc cggcatccgg gtacacgata atctctgctt tcgcattagc
     2461 agcccgcagc gcctggcgca tggtttcaac gctctcctgc ggaatgctgt tatcctgact
     2521 gccatataag ccgagaaccg gcgcgttaag atcggttgcg atatcaacag gttgtttcgg
     2581 tgaattcagc gacttgtcgc ccgtcagttt gccgtaccac gccactgcgg cttttagctg
     2641 tggattatgc gcggcataca gccaggtgat acgtccaccc cagcagaatc cggtgatcat
     2701 taaacgatga acatcgccgc cgttgcggga agcccaactg gcgacatgat cgagatcggc
     2761 cagcacctgc gagtcaggca cttttgctac cagaccgcta agcaacgtgg ggatatcggc
     2821 aaaatcattc ggatcgcctt cgcggaagta aagttcaggt gcgatagcca gatacccctc
     2881 cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc acgccaaaaa tttcctgcac
     2941 tacaatgacc actggcagtg ggccatcgct ttgctttggt ctggcatggt aagcaggcat
     3001 gttatcccct tgtgaaggga tagaggtgaa gcccgccaca atcgcgtcgt ccggggtctg
     3061 aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat ccagattgtt gtgttgttgc
     3121 catggtattc tccgtaccct tataaaaatg ttgcgcaatg ttaactatag tcagcatgca
     3181 acaaatcaca ttgcctgaat cggctcatct tttatgcagt cctgcagaat gaagagtgat
     3241 ttatgtgatt tgaatcactt ttggtgggta aatttatgca acgcatttgc gtcatggtga
     3301 tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt ctttttgctt cttctgacta
     3361 aaccgattca cagaggagtt gtatatgtcc aagtctgatg tttttcatct cggcctcact
     3421 aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg gcgacccgga tcgtgtggaa
     3481 aagatcgccg cgctgatgga taagccggtt aagctggcat ctcaccgcga attcactacc
     3541 tggcgtgcag agctggatgg taaacctgtt atcgtctgct ctaccggtat cggcggcccg
     3601 tctacctcta ttgctgttga agagctggca cagctgggca ttcgcacctt cctgcgtatc
     3661 ggtacaacgg gcgctattca gccgcatatt aatgtgggtg atgtcctggt taccacggcg
     3721 tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc tggaattccc ggctgtcgct
     3781 gatttcgaat gtacgactgc gctggttgaa gctgcgaaat ccattggagc gacaactcac
     3841 gttggcgtga cagcttcttc tgataccttc tacccaggtc aggaacgtta cgatacttac
     3901 tctggtcgcg tagttcgtca ctttaaaggt tctatggaag agtggcaggc gatgggcgta
     3961 atgaactatg aaatggaatc tgcaaccctg ctgaccatgt gtgcaagtca gggcctgcgt
     4021 gccggtatgg tagcgggtgt tatcgttaac cgcacccagc aagagatccc gaatgctgag
     4081 acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg tggaagcggc gcgtcgtctg
     4141 ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg ccttttgtat ttttgcgtag
     4201 cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca gcacaattct attttgtgcg
     4261 ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat catggtttac gcagttattg
     4321 cgttggtggg tgtggcaatt ggctggctgt ttgccagtta tcaacatgcg cagcaaaaag
     4381 ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt aagcgcggca aaacaacaaa
     4441 ttacccaaag cgagcactgg cgtgcagagt gcgagttact caataacgaa gtgcgcagcc
     4501 tgcaaagtat taacacctct ctggaggccg atctgcgtga agtaaccacg cggatggaag
     4561 ccgcacagca acatgctgac gataaaattc gccagatgat taacagcgag cagcgcctca
     4621 gtgagcagtt tgaaaacctc gccaaccgta tttttgagca cagcaatcgc cgggttgatg
     4681 agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct acgtgaacaa ctggacggtt
     4741 tccgccgtca ggttcaggac agcttcggta aagaagcaca agaacgccat accctgaccc
     4801 acgaaattcg caatctccag caactcaacg cgcaaatggc ccaggaagcg atcaacctga
     4861 cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg gggcgaggta gtattgacgc
     4921 gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata tgaaacccag gtcagcatcg
     4981 aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt gcgcctgccg cagggaaaag
     5041 atgtggtgat cgacgccaaa atgacgctgg tcgcctatga acgctatttt aacgccgaag
     5101 acgactacac ccgcgaaagc gcgctacagg aacatatcgc gtcggtgcgt aaccatatcc
     5161 gtttgctggg acgcaaagat tatcaacagc tgccggggct gcgaactctg gattacgtgc
     5221 tgatgtttat tcccgttgaa cccgcttttt tactggcgct tgaccgccag ccggagctga
     5281 tcaccgaagc gttgaaaaac aatatcatgc tggttagccc gactacgctg ctggtggcgc
     5341 tgcgcactat cgccaacctg tggcgttatg agcatcaaag ccgcaacgcc cagcaaatcg
     5401 ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt catcgatgac atgtccgcga
     5461 ttggtcaaag tctcgacaaa gcgcaggata attatcggca ggcaatgaaa aaactctctt
     5521 cagggcgcgg aaatgtgctg gcgcaggcag aagcgtttcg cggtttagga gtagaaatta
     5581 aacgcgagat taattcggat ttggctgaac aggcggtgag ccaggatgaa gagtatcgac
     5641 ttcggtcggt tccggagcag ccgaatgatg aagcttatca acgcgatgat gaatataatc
     5701 agcagtcgcg ctagcccatt gggagtagtt aagccgggta gaaatctagg gcatcgacgc
     5761 ccaatctgtt acacttctgg aacaattttt tgatgagcag gcattgagat ggtggataag
     5821 tcacaagaaa cgacgcactt tggttttcag accgtcgcga aggaacaaaa agcggatatg
     5881 gtcgcccacg ttttccattc cgtggcatca aaatacgatg tcatgaatga tttgatgtca
     5941 tttggtattc atcgtttgtg gaagcgattc acgattgatt gcagcggcgt acgccgtggg
     6001 cagaccgtgc tggatctggc tggtggcacc ggcgacctga cagcgaaatt ctcccgcctg
     6061 gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg aatccatgct caaaatgggc
     6121 cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg agtatgttca ggcgaacgct
     6181 gaggcgctgc cgttcccgga taacaccttt gattgcatca ccatttcgtt tggtctgcgt
     6241 aacgtcaccg acaaagataa agcactgcgt tcaatgtatc gcgtgctgaa acccggcggc
     6301 cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc cgctgagcaa agcctatgat
     6361 gcatactcct tccatgtgct gccgcgtatt ggctcactgg tcgcgaacga cgccgacagc
     6421 taccgttatc tggcagaatc catccgtatg catcccgatc aggataccct gaaagccatg
     6481 atgcaggatg ccggattcga aagtgtcgac tactacaatc tgacggcagg ggttgtggcg
     6541 ctgcatcgtg gttataagtt ctgacaggag accggaaatg ccttttaaac ctttagtgac
     6601 ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc tcacccgcgc tgaaaacggc
     6661 ccgctcgcgt ctgttgggca aagtgctgcg cgtggaggta aaaggctttt cgacgtcatt
     6721 gattctggtg ttcagcgaac gccaggttga tgtactgggc gaatgggcag gcgatgctga
     6781 ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt cgcgatcgcc agcagcttgc
     6841 cgccctgatt cgcagtggtg agctggaagt gcagggcgat attcaggtgg tgcaaaactt
     6901 cgttgcgctg gcagatctgg cagagttcga ccctgcggaa ctgctggccc cttatatcgg
     6961 tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga ggcgcaaagt tcctgcatca
     7021 cggcattaag cgccagcaac gttatgtggc ggaagccatt actgaagagt ggcgtatggc
     7081 acccggtccg cttgaagtgg cctggtttgc ggaggagacg gctgccgtcg agcgtgctgt
     7141 tgatgccctg accaaacggc tggaaaaact ggaggctaaa tgacgccagg tgaagtacgg
     7201 cgcctatatt tcatcattcg cactttttta agctacggac ttgatgaact gatccccaaa
     7261 atgcgtatca ccctgccgct acggctatgg cgatactcat tattctggat gccaaatcgg
     7321 cataaagaca aacctttagg tgagcgacta cgactggccc tgcaagaact ggggccggta
     7381 tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc tttttccgcc gcatattgcc
     7441 gatcagctgg cgttattgca ggacaaagtt gctccgtttg atggcaagct ggcgaagcag
     7501 cagattgaag ctgcaatggg cggcttgccg gtagaagcgt ggtttgacga ttttgaaatc
     7561 aagccgctgg cttctgcttc tatcgcccag gttcataccg cgcgattgaa atcgaatggt
     7621 aaagaggtgg tgattaaagt catccgcccg gatattttgc cggttattaa agcggatctg
     7681 aaacttatct accgtctggc tcgctgggtg ccgcgtttgc tgccggatgg tcgccgtctg
     7741 cgcccaaccg aagtggtgcg cgagtacgaa aagacattga ttgatgaact gaatttgctg
     7801 cgggaatctg ccaacgccat tcagcttcgg cgcaattttg aagacagccc gatgctctac
     7861 atcccggaag tttaccctga ctattgtagt gaagggatga tggtgatgga gcgcatttac
     7921 ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg gcaccaacat gaaattgctg
     7981 gcggaacgcg gcgtgcaggt gttcttcact caggtctttc gcgacagctt tttccatgcc
     8041 gatatgcacc ctggcaacat cttcgtaagc tatgaacacc cggaaaaccc gaaatatatc
     8101 ggcattgatt gcgggattgt tggctcgcta aacaaagaag ataaacgcta tctggcagaa
     8161 aactttatcg ccttctttaa tcgcgactat cgcaaagtgg cagagctaca cgtcgattct
     8221 ggctgggtgc caccagatac caacgttgaa gagttcgaat ttgctattcg tacggtctgt
     8281 gaacctatct ttgagaaacc gctggccgaa atttcgtttg gacatgtact gttaaatctg
     8341 tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc aactggtgtt actccagaaa
     8401 accctgctct acgtcgaagg ggtaggacgc cagctttatc cgcaactcga tttatggaaa
     8461 acggcgaagc ctttcctgga gtcgtggatt aaagatcagg tcggtattcc tgcgctggtg
     8521 agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa tgccagaact gcctgaactg
     8581 gtttacgaca gtttgcgcca gggcaagtat ttacagcaca gtgttgataa gattgcccgc
     8641 gagcttcagt caaatcatgt acgtcaggga caatcgcgtt attttctcgg aattggcgct
     8701 acgttagtat taagtggcac attcttgttg gtcagccgac ctgaatgggg gctgatgccc
     8761 ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg gttggcgcaa aacacgctga
     8821 ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc ggctttgttt aatcatcatc
     8881 taccacagag gaacatgtat gggtggtatc agtatttggc agttattgat tattgccgtc
     8941 atcgttgtac tgctttttgg caccaaaaag ctcggctcca tcggttccga tcttggtgcg
     9001 tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac caaagcagga taaaaccagt
     9061 caggatgctg attttactgc gaaaactatc gccgataagc aggcggatac gaatcaggaa
     9121 caggctaaaa cagaagacgc gaagcgccac gataaagagc aggtgtaatc cgtgtttgat
     9181 atcggtttta gcgaactgct attggtgttc atcatcggcc tcgtcgttct ggggccgcaa
     9241 cgactgcctg tggcggtaaa aacggtagcg ggctggatcc gcgcgttgcg ttcactggcg
     9301 acaacggtgc agaacgaact gacccaggag ttaaaactcc aggagtttca ggacagtctg
     9361 aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg aactgaaagc gtcgatggat
     9421 gaactacgcc aggccgcgga gtcgatgaag cgttcctacg ttgcaaacga tcctgaaaag
     9481 gcgagcgatg aagcgcacac catccataac ccggtggtga aagataatga agctgcgcat
     9541 gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt cgccggaaca gaagccagaa
     9601 accacgccag agccggtggt aaaacctgct gcggacgctg aaccgaaaac cgctgcacct
     9661 tccccttcgt cgagtgataa accgtaaaca tgtctgtaga agatactcaa ccgcttatca
     9721 cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat tatcgcggtg atcgtgatat
     9781 tcctgtgtct ggtctatttc gccaatgaca tctatcacct ggtatccgcg ccattgatca
     9841 agcagttgcc gcaaggttca acgatgatcg ccaccgacgt ggcctcgccg ttctttacgc
     9901 cgatcaagct gacctttatg gtgtcgctga ttctgtcagc gccggtgatt ctctatcagg
     9961 tgtgggcatt tatcgcccca gcgctgtata agcatgaacg tcgcctggtg gtgccgctgc
    10021 tggtttccag ctctctgctg ttttatatcg gcatggcgtt cgcctacttt gtggtctttc
    10081 cgctggcatt tggcttcctt gccaataccg cgccggaagg ggtgcaggta tccaccgaca
    10141 tcgccagcta tttaagcttc gttatggcgc tgtttatggc gtttggtgtc tcctttgaag
    10201 tgccggtggc aattgtgctg ctgtgctgga tggggattac ctcgccagaa gacttacgca
    10261 aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg gatgttgctg acgccgccgg
    10321 atgtcttctc gcaaacgctg ttggcgatcc cgatgtactg tctgtttgaa atcggtgtct
    10381 tcttctcacg cttttacgtt ggtaaagggc gaaatcggga agaggaaaac gacgctgaag
    10441 cagaaagcga aaaaactgaa gaataaattc aaccgcccgt cagggcggtt gtcatatgga
    10501 gtacaggatg tttgatatcg gcgttaattt gaccagttcg caatttgcga aagaccgtga
    10561 tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg ctactcatca ccggcactaa
    10621 cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag tattcatcct gttggtcaac
    10681 ggcgggcgta catcctcacg acagcagcca gtggcaagct gcgactgaag aagcgattat
    10741 tgagctggcc gcgcagccag aagtggtggc gattggtgaa tgtggtctcg actttaaccg
    10801 caacttttcg acgccggaag agcaggaacg cgcttttgtt gcccagctac gcattgccgc
    10861 agatttaaac atgccggtat ttatgcactg tcgcgatgcc cacgagcggt ttatgacatt
    10921 gctggagccg tggctggata aactgcctgg tgcggttctt cattgcttta ccggcacacg
    10981 cgaagagatg caggcgtgcg tggcgcatgg aatttatatc ggcattaccg gttgggtttg
    11041 cgatgaacga cgcggactgg agctgcggga acttttgccg ttgattccgg cggaaaaatt
    11101 actgatcgaa actgatgcgc cgtatctgct ccctcgcgat ctcacgccaa agccatcatc
    11161 ccggcgcaac gagccagccc atctgcccca tattttgcaa cgtattgcgc actggcgtgg
    11221 agaagatgcc gcatggctgg ctgccaccac ggatgctaat gtcaaaacac tgtttgggat
    11281 tgcgttttag agtttgcgga actcggtgtt cttcacactg tgcttaatct ctttattaat
    11341 aagattaagc aatagcatgg agcgagcctc accatcgggt tcggtgaaaa tggcctgaaa
    11401 gccttcgaac gcgccttcgg taataatcac cttatcaccc ggataagggg ttgccggatc
    11461 gacaatgtct ttcggtttat ataccgatag ctgatgaatc accgccgatg ggactatcgc
    11521 tggcgacgcg ccaaagcgca cgaagtggct gacaccgcgg gtcgcgttga tagtcgtggt
    11581 atgaatcact tctggatcaa attccacaaa caggtagttg gggaacaatg gctcactgac
    11641 tgcagtacgt ttgccacgca cgattttttc cagggtgatc atcggtgcca ggcaattcac
    11701 agcctgtctt tcgaggtgtt cctgggcacg ttgaagttgc ccgcgcttgc agtacagtaa
    11761 ataccaggat tgcataatga ctcttatccg tttaatcggg gcgcaaggat agcaaaagct
    11821 ttacgctaag ttaattatat tccccggttt gcgttatacc gtcagagttc acgctaattt
    11881 aacaaattta cagcatcgca aagatgaacg ccgtataatg ggcgtagatt aagaggctac
    11941 aatggacgcc atgaaatata acgatttacg cgacttcttg acgctgcttg aacagcaggg
    12001 tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg gaaatcactg aaattgctga
    12061 ccgcactttg cgtgccggtg ggcctgcgct gttgttcgaa aaccctaaag gctactcaat
    12121 gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg gcgatgggca tggggcagga
    12181 agatgtttcg gcgctgcgtg aagttggtaa attattggcg tttctgaaag agccggagcc
    12241 gccaaaaggt ttccgcgacc tgtttgataa actgccgcag tttaagcaag tattgaacat
    12301 gccgacaaag cggctgcgtg gtgcgccctg ccaacaaaaa atcgtctctg gcgatgacgt
    12361 cgatctcaat cgcattccca ttatgacctg ctggccggaa gatgccgcgc cgctgattac
    12421 ctgggggctg acagtgacgc gcggcccgca taaagagcgg cagaatctgg gcatttatcg
    12481 ccagcagctg attggtaaaa acaaactgat tatgcgctgg ctgtcgcatc gcggcggcgc
    12541 gctggattat caggagtggt gtgcggcgca tccgggcgaa cgtttcccgg tttctgtggc
    12601 gctgggtgcc gatcccgcca cgattctcgg tgcagtcacc cccgttccgg atacactttc
    12661 agagtatgcg tttgccggat tgctgcgcgg taccaagacc gaagtggtga agtgtatctc
    12721 caatgacctt gaagtgcccg ccagtgcgga gattgtgctg gaagggtata tcgaacaagg
    12781 tgaaacggcg ccggaagggc cgtatggcga ccacaccggt tactataacg aagtcgatag
    12841 tttcccggta tttaccgtga cgcatattac ccagcgtgaa gatgcgattt accattccac
    12901 ctataccggg cgtccgccag atgagcccgc ggtactgggc gtggcgttga acgaagtgtt
    12961 tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat ttttacctgc cgccggaagg
    13021 ctgttcttat cgtctggcgg tagtgacgat caaaaaacag tacgccggac acgcgaagcg
    13081 cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg tacactaaat ttgtgatcgt
    13141 ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg atttgggcga ttaccacccg
    13201 tatggacccg gcgcgggata ctgttctggt agaaaatacg cctattgatt atctggattt
    13261 tgcctcgcct gtctccgggc tgggttcaaa aatggggctg gatgccacga ataaatggcc
    13321 gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa gatccagatg ttgtcgcgca
    13381 tattgacgcc atctgggatg aactggctat ttttaacaac ggtaaaagcg cctgatgcgc
    13441 gtttgttttg ccctatttat cgatccgaca gagaaagcgc atgacaacct taagctgtaa
    13501 agtgacctcg gtagaagcta tcacggatac cgtatatcgt gtccgcatcg tgccagacgc
    13561 ggccttttct tttcgtgctg gtcagtattt gatggtagtg atggatgagc gcgacaaacg
    13621 tccgttctca atggcttcga cgccggatga aaaagggttt atcgagctgc atattggcgc
    13681 ttctgaaatc aacctttacg cgaaagcagt catggaccgc atcctcaaag atcatcaaat
    13741 cgtggtcgat attccccacg gagaagcgtg gctgcgcgat gatgaagagc gtccgatgat
    13801 tttgattgcg ggcggcaccg ggttctctta tgcccgctcg attttgctga cagcgttggc
    13861 gcgtaaccca aaccgtgata tcaccattta ctggggcggg cgtgaagagc agcatctgta
    13921 tgatctctgc gagcttgagg cgctttcgtt gaagcatcct ggtctgcaag tggtgccggt
    13981 ggtagaacaa ccggaagcgg gctggcgtgg gcgtactggc accgtgttaa cggcggtatt
    14041 gcaggatcac ggtacgctgg cagagcatga tatctatatt gccggacgtt ttgagatggc
    14101 gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg cgggaagatc gcctgtttgg
    14161 cgatgcgttt gcatttatct gagatataaa aaaacccgcc cctgacaggc gggaagaacg
    14221 gcaactaaaa ctttattcag tggcatttag atctatgtcg catccggcat tcggcacacg
    14281 atgcctgatg cgacgctggc gcgtcttatc aggcctacat tggtgccgga tcggtagacc
    14341 ggataaggcg ttcacgccgc atccggcaag tggttaaacc cgctcaaaca ccgtcgcaat
    14401 accctgaccc agaccgatac acatcgtcgc cagaccaaac tgaacgtctt tgtgttccat
    14461 cagattcagc agcgtggtgc tgatacgcgc accggaacaa cccagcggat gacccagcgc
    14521 gatcgcgcca ccgttgaggt tgatcttctc gtcaatctgc tcaattagtc ccagatcttt
    14581 aatacatggc aggatctgcg cggcaaaggc ttcattcatt tcaaacacgc cgatatcact
    14641 gacagaaagc cccgcttttt tcagcgccag cttcgaggcc ggaaccgggc cgtaacccat
    14701 aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca cgagcgcgcg gcttaagacc
    14761 taattcatgg gcgcggcttt cactcatcac cagcatggca gctgcgccat cggaaagtgc
    14821 agaagatgtg cccgccgtta ccgtaccgtt tactggatca aacgccggac gcagcgtggc
    14881 gagggcttcc acggtggttt ccgggcgaat cacttcgtcg taattaaact gcttcaggac
    14941 gccgtcggca tcgtgaccac cagttgggat gatttcattt ttaaatgcgg ccgactgcgt
    15001 ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca tcctgcattt cacggctgat
    15061 accgtgcata cgcgccagca tttctgccgt taagcccatc atgcccgccg ctttggcgac
    15121 attgcggctc aggccggggt gaaaatcgac gccgtgactc atcggcacat ggcccatatg
    15181 ctccacgccg ccaaccagac atgcctgcgc atcgccggtc atgatcattc gtgctgcgtc
    15241 atgcagtgcc tgcatggatg aaccacacaa gcgattgaca gtaaccgccg ggacagagtg
    15301 cggcacttct gccagcagcg ccgcgttacg ggcgatatta aaaccctgct ccagcgtctg
    15361 ctgcacacaa ccccagtaaa tatcgtcgag ggccgccgct tccagcgccg ggttacgcgc
    15421 cagcaggcta cgcattaaat gagcggagag atcttctgca cgcacgttac gaaaagcacc
    15481 gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca atgacaacct gttccattgt
    15541 gactccttaa gccgttttca ggtcgccaac cggacgggct ggctcaaccg ggggatagta
    15601 cggttcgtta tgacgcgctt tattacgcag accttccggc acttcgtaca gcgggccgag
    15661 gtgctgatat tgctgtgcca tatcgaggta tttcgcgctg ccgagggtgt ccagccagcg
    15721 gaacgcgccg ccgtggaacg gagggaagcc caggccgtag accagcgcca tatccgcttc
    15781 tgccggagtg gcgataatgc cttcttccag acaacgcacc acttcgttga ccatcgggat
    15841 catcatgcgg gcgataatct cttcttcgct gaaatcgcgc ttcggctggc tcacttctac
    15901 tagcaggtcg tcaacagcgg cgtcttcttc tttcttcggc ttacctttgc tgtcttcttt
    15961 ataacgccag aaaccgaggc cgttcttctg accaaagcgg ttggcatcaa acagcgcgtc
    16021 gatggcatcg cgataatctt tctgcatccg ctgcgggaag cctgctgcca tcaccgcctg
    16081 agcgtgatgc gcggtgtcaa tgcccacgac atccagcaga tatgccggac ccatcggcca
    16141 gccaaactgt ttttccatca ctttgtcgat cttgcggaaa tccgcgccgt cgcgcagcag
    16201 ctggctgaaa ccggcgaaat acgggaacag cacgcggttg acgaagaagc cggggcaatc
    16261 gttaaccaca atcggcgttt tacccatctt gctcgcccag gcgacgactt tcgcaatagt
    16321 ttcgtcggag cttttctcgc cgcgaataat ttccaccaac ggcattcggt ggaccgggtt
    16381 aaagaagtgc atcccgcaga agttttccgg acgttccagc gcgttggcca gttcgctgat
    16441 aggaatggtt gaagtgttag acgccagcac ggtatccggg cgtactttct gctcggtttc
    16501 tgccagtacg gcttttttca ctttcgggtt ttcgacaacc gcttctacca caacatccac
    16561 gcggtcaaac ccggcgtagt cgagcgttgg gtggattgta gagatcacgc cagccagttt
    16621 cagaccatcg atcttgccgc gctcaagctg cttgttcagc agtttcgcgg cttcggtcat
    16681 gccgagggtt aacgacttgt cgttgatatc tttcatgaca accggcacgc ctttccacgc
    16741 agactggtaa gcgatgccgc cgcccataat gcctgcgccc agcacagcgg cctgtttcgg
    16801 ggtttcaacg tctttggtga gtttcttcgc tttgcctttt acatattgat cgttaaggaa
    16861 tatgccgacc agtgcgcgag cttcgttggt atgcgccagc gggacaaaac ttttgttttc
    16921 caggtttaga gcttcttcac gaccaaaacg ggccgcagct tcaatggttt ttactgcggt
    16981 gatgggggcc ggataatgtt tccccgccgt ttgtgcgacc atccctttag cgatggtgaa
    17041 gctcatggta gcttcaatct tgctcagttt tagcggttcc agcttcggct gacgtttcgc
    17101 tttccagtcg aggtcgccgt taatggcctg gcgtaaaatc gccatcgccc cttcaatcag
    17161 tttttctgct ttgactacgc catccaccag accaattttc agcgcctggt ccgcgccgac
    17221 atctttaccg gcggcaatga tttccagcgc actgtcagcg cccagcatac gtggcatacg
    17281 tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt tccggcagac cgatgcgcag
    17341 atccggcgtc gccagacgat aatcggtcgc cagcacgcat tcgcagccgc cacccagcgc
    17401 gtagccgttg acggcagcaa tggtcggcac cggcagatct tccaggcgat taaacacgct
    17461 attggcaaaa tgcagccact ggcttaactg ttcttcagga acgaggaaca gggacaaaaa
    17521 ttcggtgata tcagcaccga cgataaaggc tgctttgttc gaacgcagca gcagcccttt
    17581 tagatctgat tgctgttcca gcacgccgat ggcctcgccg aggctggcga cggtcgcagt
    17641 gtcgagttta ttaactgaac ctggggcatc aaataccagt tcggcaatgc catcttccag
    17701 ccagtcaagg tacagggtgt cgcctttgta aagcatgtca gtctcctgaa tccgcaaggt
    17761 gatctggtcg taccagatga gtcgaagtgt gtattttgtg ttaaaaatat gcaaacaaaa
    17821 gattaaagaa atgccgatct gatcacgctc ggcagaaatc acgctctgga tgaacgatgt
    17881 gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt aaaaaatgga atcactggcc
    17941 tcgctctata aaaatcatat agctacctta caagaacgga ctcgcgatgc gctggcgcgc
    18001 ttcaagctgg atgcgttact tattcactcc ggcgagctgt tcaatgtttt tctcgacgat
    18061 catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt gggtgccggt aactcaggtg
    18121 ccaaactgct ggttgctggt ggatggcgtg aacaagccga aactgtggtt ttatctgccg
    18181 gttgattact ggcacaacgt cgaaccgctg ccgacctcct tctggactga agatgtggaa
    18241 gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc tgcctgctgc gcgcggcaat
    18301 atcggttata tcggtccggt gccggagcgt gcgctgcaac tgggtattga ggccagcaac
    18361 atcaacccga aaggggttat cgactacctg cattactatc gctccttcaa aaccgagtac
    18421 gaactggcct gtatgcgtga agcgcagaaa atggcggtca acggtcatcg tgcggcagaa
    18481 gaagcgttcc gttctggcat gagcgagttc gatatcaaca ttgcctatct gaccgcgact
    18541 ggtcatcgtg ataccgacgt accttacagc aacattgtgg cgcttaacga acacgctgcg
    18601 gtgctgcatt acaccaaact ggatcatcag gcgccggaag agatgcgcag cttcctgctg
    18661 gatgccgggg ccgaatataa cggctatgcc gctgacctga cccgtacctg gtcggcaaaa
    18721 agtgacaacg attacgcaca gctggtgaaa gacgtaaatg atgaacaact ggcgctgatc
    18781 gcgaccatga aagctggcgt tagctatgtg gattaccaca tccagttcca tcagcgcatc
    18841 gccaaattgc tgcgtaaaca tcaaatcatc accgatatga gtgaagaagc gatggtcgaa
    18901 aacgatctca ccggaccgtt tatgccgcac ggtatcggcc atccgctggg cctgcaggtg
    18961 catgacgtcg ccggttttat gcaggatgat agcggtacgc acctcgcggc accggcaaaa
    19021 tatccgtacc tgcgctgcac ccgtattctc cagccgggca tggtgttaac catcgaaccg
    19081 ggtatctact tcattgaatc gctactggca ccgtggcgtg aagggcagtt cagcaagcac
    19141 ttcaactggc agaaaattga agcactgaaa ccgttcggcg gcattcgtat cgaagacaac
    19201 gtggtgatcc acgaaaataa cgtggaaaac atgacccggg atctgaaact ggcgtgatgg
    19261 aaagctggtt aattcctgcg gcaccggtca cggtcgttga agagatcaaa aaaagccgct
    19321 tcataacgat gttggcgcat accgatggcg ttgaggcggc gaaagcgttt gttgaatcgg
    19381 tgcgggcaga acatcccgat gcccgccacc attgtgtggc gtgggtggcg ggtgcgccgg
    19441 atgattcaca acagttgggt ttctctgacg acggggaacc ggcgggaacg gcaggtaaac
    19501 cgatgctcgc ccaactgatg ggcagcggcg tcggggaaat taccgccgtg gtggtgcgct
    19561 actacggcgg catattgctt ggcaccggtg ggttagtgaa agcgtatggc ggcggcgtga
    19621 atcaggcgct gcggcagcta acgacccaac gcaagacgcc attaaccgaa tatactttgc
    19681 aatgtgaata tagtcagtta accggcattg aagcgttgct ggggcagtgt gacggcaaaa
    19741 ttatcaacag tgattatcag gcattcgttc tgctgcgggt ggcgcttccg gcggcgaaag
    19801 tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg ttcattgcaa ttgttagcga
    19861 ttgaagaata atccccactt cgttttgcag aactaaggaa gcggcagaga tgcattttcg
    19921 cgccattacc cgaatcgttg gactactggt catcttattt tcagggacca tgattatccc
    19981 tgggctggta gcactcatct accgggatgg agcgggccgc gcttttaccc agaccttttt
    20041 tgtcgccctc gccattggct ctatgctgtg gtggccgaac cgcaaagaga agggcgaact
    20101 gaaatcccgt gaggggtttc tgatagtggt gctgttctgg accgtgctgg gtagtgtcgg
    20161 tgcgctccct tttatcttct cggaaagccc gaacctcacg attaccgatg cgttttttga
    20221 atctttctct ggcctgacca ccaccggggc cactacgctg gtggggctgg attcgctccc
    20281 tcacgccatc ctcttttatc gccagatgct gcaatggttt ggcgggatgg ggatcatcgt
    20341 gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg atgcagctct atcgtgcaga
    20401 aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga attgcggaaa cggcgaaaac
    20461 cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg ctggcgttgt ggtttgccgg
    20521 aatggatgcc tttgatgcca tcggccatag ctttgcgact atcgctattg gcggcttctc
    20581 gacacatgat gccagtatcg gttatttcga tagcccgact attaacacta tcattgctat
    20641 cttcctgctg atctccggct gtaactacgg tctgcacttt tcactgttaa gtgggcgtag
    20701 tctgaaggtt tattggcgcg atccggaatt tcgcatgttt atcggcgtac agtttacgct
    20761 ggtggttatt tgtaccctcg tactgtggtt tcataatgtc tacagttcgg cgctgatgac
    20821 aattaaccag gcgtttttcc aggtggtgtc gatggcgaca accgccgggt ttacgactga
    20881 cagcattgcc cgctggccgc tctttttgcc ggtactgctt ttatgttcag cttttatcgg
    20941 cggttgtgcc gggtcaacgg gcggtggcct gaaagtgatc cgcatcctgc tgctgtttag
    21001 gcaggggaac cgtgagctga aacgactggt gcatccgaac gccgtctata gcattaagct
    21061 ggggaatcgc gcactgccgg aacgtatcct cgaagccgtc tggggatttt tctccgccta
    21121 tgcattggtg tttattgtca gtatgctggc gattatcgcc acgggcgtgg atgacttttc
    21181 tgcctttgcc tcggttgttg cgacattgaa taacctgggg ccagggcttg gcgtggttgc
    21241 tgataacttt accagcatga acccggtggc taaatggatc ctgattgcca acatgctgtt
    21301 tggccgtctc gaggtcttta cattgctggt gctctttacc ccgactttct ggcgtgaatg
    21361 atggagtaat acgtgaaaac attaattctt ttctcaacaa gggacggaca aacgcgcgag
    21421 attgcctcct acctggcttc ggaactgaaa gaactgggga tccaggcgga tgtcgccaat
    21481 gtgcaccgca ttgaagaacc acagtgggaa aactacgacc gtgtggtcat tggtgcttct
    21541 attcgctatg gtcactacca ctctgcgttc caggaatttg tcaaaaaaca tgcgacgcga
    21601 ctgaattcga tgccgagcgc cttttattcc gtaaacctgg tggcgcgcaa accggagaag
    21661 cgtactccac agaccaacag ctacgcgcgg aagtttctga tgaactcgca atggcgtccc
    21721 gatcgctgcg cggtcattgc cggggcgctg cgttacccac gttatcgctg gtacgaccgt
    21781 tttatgatca agctgattat gaagatgtca ggcggtgaaa cggatacgcg caaagaagtt
    21841 gtctataccg attgggagca ggtggcgaat ttcgcccgag aaatcgccca tttaaccgac
    21901 aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc ggtc
//
LOCUS       DAZKFU020000061        21911 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZKFU020000061 DAZKFU020000000
VERSION     DAZKFU020000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.061"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHOQQ_RS09075"
                     /old_locus_tag="pgaptmp_001815"
     CDS             complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHOQQ_RS09075"
                     /old_locus_tag="pgaptmp_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HGU4470079.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLALLSTISG
                     SLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNR"
     gene            1263..1799
                     /gene="sutR"
                     /locus_tag="ACHOQQ_RS09080"
                     /old_locus_tag="pgaptmp_001816"
     CDS             1263..1799
                     /gene="sutR"
                     /locus_tag="ACHOQQ_RS09080"
                     /old_locus_tag="pgaptmp_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGU4470080.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHOQQ_RS09085"
                     /old_locus_tag="pgaptmp_001817"
     CDS             1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHOQQ_RS09085"
                     /old_locus_tag="pgaptmp_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGU4470081.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTDPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHOQQ_RS09090"
                     /old_locus_tag="pgaptmp_001818"
     CDS             complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHOQQ_RS09090"
                     /old_locus_tag="pgaptmp_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGU4470082.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4377..4553
                     /gene="hicA"
                     /locus_tag="ACHOQQ_RS09095"
                     /old_locus_tag="pgaptmp_001819"
     CDS             4377..4553
                     /gene="hicA"
                     /locus_tag="ACHOQQ_RS09095"
                     /old_locus_tag="pgaptmp_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
                     /protein_id="HGU4470083.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKVRFHGMHSVMPRHPCDEIK
                     EPLRKAIQKQLGLS"
     gene            4599..5015
                     /gene="hicB"
                     /locus_tag="ACHOQQ_RS09100"
                     /old_locus_tag="pgaptmp_001820"
     CDS             4599..5015
                     /gene="hicB"
                     /locus_tag="ACHOQQ_RS09100"
                     /old_locus_tag="pgaptmp_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGU4470084.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHOQQ_RS09105"
                     /old_locus_tag="pgaptmp_001821"
     CDS             5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHOQQ_RS09105"
                     /old_locus_tag="pgaptmp_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4470085.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQVVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHOQQ_RS09110"
                     /old_locus_tag="pgaptmp_001822"
     CDS             6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHOQQ_RS09110"
                     /old_locus_tag="pgaptmp_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGU4470086.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVKQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHOQQ_RS09115"
                     /old_locus_tag="pgaptmp_001823"
     CDS             7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHOQQ_RS09115"
                     /old_locus_tag="pgaptmp_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4470087.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVLVSWSRDVMVPLV
                     EER"
     gene            8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHOQQ_RS09120"
                     /old_locus_tag="pgaptmp_001824"
     CDS             8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHOQQ_RS09120"
                     /old_locus_tag="pgaptmp_001824"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4470088.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPIAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHOQQ_RS09125"
                     /old_locus_tag="pgaptmp_001825"
     CDS             9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHOQQ_RS09125"
                     /old_locus_tag="pgaptmp_001825"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4470089.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10669..12093
                     /gene="patD"
                     /locus_tag="ACHOQQ_RS09130"
                     /old_locus_tag="pgaptmp_001826"
     CDS             10669..12093
                     /gene="patD"
                     /locus_tag="ACHOQQ_RS09130"
                     /old_locus_tag="pgaptmp_001826"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGU4470090.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12190..12285)
                     /gene="yncL"
                     /locus_tag="ACHOQQ_RS09135"
                     /old_locus_tag="pgaptmp_001827"
     CDS             complement(12190..12285)
                     /gene="yncL"
                     /locus_tag="ACHOQQ_RS09135"
                     /old_locus_tag="pgaptmp_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGU4470091.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            12480..12653
                     /gene="ortT"
                     /locus_tag="ACHOQQ_RS09140"
                     /old_locus_tag="pgaptmp_001828"
     CDS             12480..12653
                     /gene="ortT"
                     /locus_tag="ACHOQQ_RS09140"
                     /old_locus_tag="pgaptmp_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGU4470092.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            12739..12972
                     /gene="ydcY"
                     /locus_tag="ACHOQQ_RS09145"
                     /old_locus_tag="pgaptmp_001829"
     CDS             12739..12972
                     /gene="ydcY"
                     /locus_tag="ACHOQQ_RS09145"
                     /old_locus_tag="pgaptmp_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGU4470093.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(12973..13422)
                     /gene="ydcZ"
                     /locus_tag="ACHOQQ_RS09150"
                     /old_locus_tag="pgaptmp_001830"
     CDS             complement(12973..13422)
                     /gene="ydcZ"
                     /locus_tag="ACHOQQ_RS09150"
                     /old_locus_tag="pgaptmp_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4470094.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13419..13937)
                     /gene="mddA"
                     /locus_tag="ACHOQQ_RS09155"
                     /old_locus_tag="pgaptmp_001831"
     CDS             complement(13419..13937)
                     /gene="mddA"
                     /locus_tag="ACHOQQ_RS09155"
                     /old_locus_tag="pgaptmp_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGU4470095.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14118..15155
                     /gene="curA"
                     /locus_tag="ACHOQQ_RS09160"
                     /old_locus_tag="pgaptmp_001832"
     CDS             14118..15155
                     /gene="curA"
                     /locus_tag="ACHOQQ_RS09160"
                     /old_locus_tag="pgaptmp_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGU4470096.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVEESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKIFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15353..16018
                     /gene="mcbR"
                     /locus_tag="ACHOQQ_RS09165"
                     /old_locus_tag="pgaptmp_001833"
     CDS             15353..16018
                     /gene="mcbR"
                     /locus_tag="ACHOQQ_RS09165"
                     /old_locus_tag="pgaptmp_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGU4470097.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16054..18156)
                     /gene="pqqU"
                     /locus_tag="ACHOQQ_RS09170"
                     /old_locus_tag="pgaptmp_001834"
     CDS             complement(16054..18156)
                     /gene="pqqU"
                     /locus_tag="ACHOQQ_RS09170"
                     /old_locus_tag="pgaptmp_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGU4470098.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLIGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            18398..19459
                     /gene="yncE"
                     /locus_tag="ACHOQQ_RS09175"
                     /old_locus_tag="pgaptmp_001835"
     CDS             18398..19459
                     /gene="yncE"
                     /locus_tag="ACHOQQ_RS09175"
                     /old_locus_tag="pgaptmp_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGU4470099.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISVI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(19574..21073)
                     /gene="ansP"
                     /locus_tag="ACHOQQ_RS09180"
                     /old_locus_tag="pgaptmp_001836"
     CDS             complement(19574..21073)
                     /gene="ansP"
                     /locus_tag="ACHOQQ_RS09180"
                     /old_locus_tag="pgaptmp_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGU4470100.1"
                     /translation="MSKHNTDTSEQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            21341..>21911
                     /gene="yncG"
                     /locus_tag="ACHOQQ_RS09185"
                     /old_locus_tag="pgaptmp_001837"
     CDS             21341..>21911
                     /gene="yncG"
                     /locus_tag="ACHOQQ_RS09185"
                     /old_locus_tag="pgaptmp_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGU4470101.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVC"
BASE COUNT         5549 a         5421 c         5656 g         5285 t
ORIGIN      
        1 atcggtttct gtcagcgatt aaattcaaca ccacgtaaca aacgcctccg gcaattaatc
       61 cccagaacgc agaaccaatc ccgaccagcg tcaatccact tgccgttacc agaaatgcca
      121 ccaccgccgc gtctcgctca cgctcattat gcagcgcctg atacaaactg ccgctaatgg
      181 tacttaacag cgccagacct gccagcatct ggatccaact tacgggcagg gcagccatca
      241 tcccggtaat agcactacca aacagacctg cgatcaaata aaaaatgcct gcaacggcag
      301 ctgccagcca acgttgatct ttatccggat gcgcttccgg gctttggcaa atagccgcgg
      361 tgattgccgc aataccgacg gaataaacgc cgaaagggga aaaaaccagt gccagcaatc
      421 cagtaaatac aattaatggc gaaacaggag ccgaataccc ggctgctttc attgctgcga
      481 tacccggtgc gttttgcgat gccatcgtca ccagaaaaag ggggagtgca acgctcaggc
      541 tgtgagcaaa cgaaaaatca ggggtaatat aagtggggag aacgggttta aagacaacat
      601 cagttgtgac aacgtcacct tgcgcgataa cgatcacgat cccaataatc atcgcggcaa
      661 ttaccgcata gcgcggcgca acggccttgg ttgccagcca taccagcaac atacttccac
      721 acaacgtaaa ttgaccgtcc agactggcaa acgcctgtaa accaaagcgt aataaaatcc
      781 cggcaagcat tgccgccgca agcgagtgcg gaataatgcg catcagacga gcaaagagtc
      841 ccgttatgcc gcagaggact attaacgcgt tggtgacaat aaaaacgccg atggcttcgt
      901 taagtgttag tccctgcaat ccggtgacca acaaagccgc gccaggcgtt gaccatgcgg
      961 tgagaacagg tacgcgatac cataatgtca gagtcagcgt actgacgccc attgccagcc
     1021 ccagcgccgt catccagcca gagatttgtg cagtggtggc tccggcgaca atcgctgctt
     1081 gccagattat tgccgctgaa ctggcgtagc caattaatac cgccagaaac cccgccagta
     1141 gcgtgggtgg aggaatagaa aacagacgca tagtcacctc gtgcgttata gcgtacaggc
     1201 accgtagcat ttgtccgtta taacgcacaa gtgataaact tccgttttgc cggaggagtc
     1261 gcatggaaaa tctcgctcgc tttttatcca ccacacttaa acaactacgt cagcagcgcg
     1321 gctggagtct ttcgcgactg gcagaagcga cgggcgtttc taaagcaatg ttaggacaga
     1381 ttgagcgtaa tgagtccagc ccgacggtcg cgacattatg gaaaattgcc actgggttga
     1441 acgtgccatt ttcgacattt atttctccgc cacagtccgc tacgccttca gtttatgatc
     1501 cacaacagca ggcgatggtg atcatttcgc tgtttcctta tgatccacaa ctctgctttg
     1561 aacacttctc gattcagatg gcacccggtg caataagcga atcgacgccc catgagaagg
     1621 gggtaatcga acatgtcgtc gtcattgatg gacaacttga tctgtgcgtt gatggtgaat
     1681 ggcagaccct taattgtggg gaaggcgttc gatttgctgc agacgtcacg catatctatc
     1741 gtaatggtgg ggagcaaacc gtacattttc attccctcat ccattacccg cgcagttaag
     1801 cgggaaaact atttcgcaac gtcgcgcttg tggctaaaat agccgccatt tttcagctac
     1861 tggataagaa tgtgaccgta tcttctcatc gacttgaact gttaagcccg gcacgcgatg
     1921 ccgccattgc ccgcgaagct attttgcacg gtgccgatgc tgtttatatc ggcggccctg
     1981 gttttggtgc ccgtcataat gccagtaata gcctgaaaga tattgccgag ctggtgccgt
     2041 ttgcccatcg ttatggtgca aaaattttcg tcacgcttaa caccattttg catgatgatg
     2101 agctggaacc cgcgcaacgg ctgattactg acctctacca gaccggtgtc gatgcgctga
     2161 ttgttcagga tatggggatt ctggaacttg atattccgcc gattgaactg cacgccagta
     2221 cgcagtgcga cattcgtaca gtagaaaaag cgaagttcct ttctgatgtt ggcttcacgc
     2281 agattgtgct ggcgcgagag ctgaatcttg atcagatccg cgcgattcac caggccacgg
     2341 acgcgaccat tgaattcttt attcatggcg cactgtgcgt ggcctattcg ggtcagtgct
     2401 acatttctca tgcgcaaaca gggcgtagcg ccaaccgtgg cgattgctcg caggcgtgcc
     2461 gtttgccata cacattgaaa gacgatcagg ggcgggtggt ttcctatgaa aaacatctgc
     2521 tgtcgatgaa agataacgat cagactgcca acctcggcgc gctgattgat gctggcgtac
     2581 gctccttcaa gattgaaggg cgttacaaag atatgagcta cgtgaagaat atcaccgccc
     2641 attatcgcca gatgctggat gccattattg aagaacgtgg cgatctgacg cgcgcttcat
     2701 caggtcgtac tgaacatttc tttgttccat cgacggaaaa gactttccac cgtggtagca
     2761 cagattattt tgtgaatgcc cgtaaaggcg atattggcgc gttcgattcg ccgaaattta
     2821 tcggcctgcc ggtgggtgaa gtattgaaag tggcgaaaga tcatctcgat gttgccgtta
     2881 ccgatccact ggcaaatggc gatggcctga acgtgttgat taaacgtgaa gtcgtcggtt
     2941 ttcgtgccaa tacggtcgag aaaaccggag aaaatcagta ccgcgtctgg cccaatgaaa
     3001 tgccagcaga tttgcacaaa attcgcccgc atcacccact aaaccgtaat cttgatcata
     3061 actggcagca ggcactgaca aaaacctcca gcgaacgtcg ggtggcggta gacattgaac
     3121 tgggcggctg gcaggaacaa ctgattctga ccctcaccag tgaagagggg gtcagcatca
     3181 cgcatacgct ggatgggcag ttcgacgaag ccaataacgc cgaaaaagca atgaacaatc
     3241 tgaaggatgg tctggcaaaa ctggggcaaa ccatctatta cgcccgcgat gtgcaaatta
     3301 atttgccggg ggcgctgttt gtaccaaaca gtctgttaaa ccagttccgc cgtgaagctg
     3361 ctgacatgct ggatgctgcg cgtcttgcca gttaccagcg cggcagccgt aaaccggttg
     3421 ctgatcctgc gccggtttat cctcaaacac atctgagttt cctcgcgaac gtttacaacc
     3481 agaaagcgcg tgaattttat catcgctatg gtgtgcagct gattgacgcg gcgtatgagg
     3541 cacatgaaga gaagggcgaa gtcccggtga tgatcaccaa gcattgtctg cgctttgcct
     3601 ttaatctgtg cccgaaacag gcgaaaggca atatcaaaag ctggaaggcg acgccaatgc
     3661 aactggtaaa cggcgatgaa gtattaacgc taaagtttga ttgccgccca tgcgagatgc
     3721 acgtcattgg caaaatcaaa aatcacatac tgaaaatgcc gttaccggga agcgtagtgg
     3781 catccgtaag tccggatgag ctgctgaaaa cattgccgaa gcgaaaaggg taaaacgcca
     3841 gttttctggt tactcacaac ttattgaatc tgcatgatat tgcctgccgg gtaaggcgtt
     3901 acgccgcatc cggcatcaaa cgactcaacg ttgtcccgct tctggtttgc gcgatttttg
     3961 ccagtaaaaa tgctcgcgca aaccttccgc cgactcttcc gccacagcac gcaattcatc
     4021 gctgtccgct tcatgacgca gctgatgatc cacattcttt acccacacaa attcatgtcc
     4081 tttgtgccct gccatgagtt gtcctgagaa cagcgcacac gttaataaga caaccgataa
     4141 cgccttcgta aacatcctgc cacctttttc ttaccttttg ccgctatgat gccgatcgtt
     4201 tcttgaggtt attattcagt tttgcaaatt agcgcaaaga aattctggaa tcttccttcc
     4261 tgattttgca ttgcattctg ccgttgcggc aatttagtgc tattttcgag caaattacac
     4321 acggaggtaa acactaatgt ttactttgtt gatatactca gcggcaggga ggcgatgtga
     4381 aacaaagcga gttcagacgt tggctcgaat ctcagggcgt cgatgtagcg aatggcagca
     4441 accatttgaa agtcaggttt catgggatgc acagtgtcat gccgcgtcac ccctgcgatg
     4501 agattaaaga accattgcgt aaagcaatcc agaaacaact cggtttgagt taatcgccaa
     4561 ttaaaaaggt taatgacatg cgagagacag tcgaaattat gcgttatccc gtcactctta
     4621 cacccgcgcc ggaaggcggt tatatggttt cttttgtgga tatccctgaa gcgttgaccc
     4681 agggcgaaac tgtcgctgaa gcgatggaag cggcaaaaga tgctttactg accgcatttg
     4741 atttttattt tgaagataac gagcttatcc cgttaccttc gccattaaat agtcacgatc
     4801 attttattga agtacctttg agcgtcgcct ctaaggtatt gctgttaaat gcttttttac
     4861 agtcagaaat cactcagcaa gagttagcca ggcgaattgg caaacctaaa caggagatta
     4921 ctcgcctatt taacttgcat catgcgacaa aaatcgacgc cgtccagctc gcggcaaagg
     4981 cgcttggcaa agagttatcg ctggtgatgg tttaattaca gttaacgaaa agttgtcatt
     5041 tttaacaact gatatagact gccgaatcat ctgcacataa ttacgattcg ataatgaaaa
     5101 aataccagca gcttgcagaa caattacgcg agcagattgc gtcgggtatc tggcaacccg
     5161 gcgatcgttt accttcgttg cgtgaccagg tggcgctttc tggcatgagc tttatgactg
     5221 tcagccatgc ctatcagttg ctcgaaagtc agggatatat tatcgcacga ccgcagtcgg
     5281 gttattacgt tgcgccacag gcaataaaaa tgccgaaagc gccagtcatt ccagtcactc
     5341 gagatgaagc agtcgatatc aacacttata tttttgatat gttgcaggcc agtcgcgatc
     5401 cgtcggtcgt tccgtttgcc tcggcctttc ccgacccgcg acttttcccc ctccaacaac
     5461 taaaccgctc gctggcgcag gtaagcaaaa ccgccacggc gatgagtgtg attgaaaact
     5521 taccgccagg aaacgcagaa ctgcgtcagg ctattgctcg tcgctatgcc ttacagggca
     5581 tcaccatttc tcctgatgaa attgtcatta ctgccggggc gttagaggca ttaaacctca
     5641 gtttgcaagt ggtaactgaa ccgggcgatt gggtgatagt agagaatcct tgtttctacg
     5701 gtgcgttgca ggcgctggag cggctacggc tgaaggcgtt atcggtggcg acggatgtta
     5761 aagaagggat cgatcttcag gcgctggaac tggcgttgca ggagtatccg gtgaaagcgt
     5821 gctggctgat gactaatagc cagaatcctc tcggatttac cttaacgccg caaaaaaaag
     5881 cacaactggt ggcgttgctc aatcagtaca acgtaacgct gattgaagat gacgtttaca
     5941 gcgaacttta ttttggacgg gaaaaaccgc tgcctgcgaa agcgtgggat cgccacgatg
     6001 gcgttttgca ttgctcttcg ttttcgaaat gtctggtgcc tggttttcgt attggttggg
     6061 tcgccgccgg aaaacatgca cgtaaaattc aacgcttgca gttgatgagt acgctttcca
     6121 ccagctcacc gatgcagctt gcgctggtgg attacctttc cacgcgccga tacgacgccc
     6181 atcttcgtcg cctgcgtcgc cagcttgcgg aacgtaaaca acgtgcctgg caggcactgc
     6241 tgcgttacct gcctgcggaa gtgaaaattc atcataatga cagcggttat tttctctggc
     6301 tggagctccc cgagccgtta gatgccggcg aattgagcct ggcggcactg acgcatcata
     6361 tcagtattgc gccgggtaaa atgttttcta ccggtgaaaa ctggtcacgt ttttttcgtt
     6421 ttaataccgc gtggcagtgg ggagaacgtg aagaacaggc ggtaaaacaa ttaggcaaac
     6481 ttattcaaga acggctgtaa tagcgtttaa tttaattcct cttagattgg gtaatatgaa
     6541 tttcgaatag cagtcatatt tcctaactcc ttgtctatac tccagaagat aaccttacag
     6601 acggcataat gcgcggtagc tcacaacctg attaaatttt ctcaggggcg aaggtgtgcc
     6661 tgcaagccgc cgtctatggt taaacaagga gatattttta cggcacggcg gctgaacaat
     6721 taattacgac aggagtaaga ccttatgagc aagacatttg cccgcagcag cctgtgtgcg
     6781 ctcagcatga caataatgac cgctcacgcc gccgaacccc ctaccaattt agataaaccg
     6841 gaagggcgac tggatattat cgcctggccg ggatatatcg aacgcggaca aactgataaa
     6901 caatacgact gggtaaagca attcgaaaaa gagacaggct gcgcggtgaa tgtgaaaact
     6961 gcggcgactt ccgatgaaat ggtcagcctg atgaccaaag gaggttacga tctggtcacg
     7021 gcgtccggcg atgcctcgct gcgtttgatt atgggtaaac gcgtgcagcc gattaatacc
     7081 gccttgattc ccaactggaa aacgctcgat ccgcgcgtgg ttaaaggcga ctggtttaac
     7141 gttggcggca aagtttacgg cacaccttac caatgggggc cgaacctgct gatgtacaac
     7201 actaaaacct tcccgacgcc gccggatagc tggcaagtgg tttttgttga gcaaaatctg
     7261 ccggacggca agagcaataa aggccgcgtt caggcttatg atggccctat ctatattgcg
     7321 gacgctgcgt tgttcgttaa agccactcag ccgcagttgg gcatcagcga tccgtatcaa
     7381 ctcaccgaag aacagtacca ggcggtgctg aaagtgctgc gcgctcaaca cagtttgatc
     7441 catcgctact ggcatgacac taccgtgcaa atgagcgatt tcaaaaacga gggtgtggtc
     7501 gcttccagtg cctggcccta tcaggccaac gccctgaaag ccgaaggcca gcctgttgct
     7561 accgttttcc cgaaggaggg tgttaccggt tgggctgata ccaccatgct gcatagcgaa
     7621 gcgaaacatc cggtttgcgc ctacaaatgg atgaactggt cattaacgcc aaaagtgcag
     7681 ggcgatgtgg cggcctggtt tggctcgtta ccggtagtgc cggaagggtg taaagccagt
     7741 ccgttattag gcgaaaaagg ttgtgaaacc aacggtttta actatttcga caaaatcgcc
     7801 ttctggaaaa cgcctatagc agaagggggc aagtttgttc cctacagtcg ctggacgcag
     7861 gattacattg ccattatggg cggtcgctaa cttcgctggg gtgctttatg acgtacgcag
     7921 tggagtttga caacgtctcg cggttgtacg gtgacgtgcg cgcagtagat ggcgtcagta
     7981 ttgcgataaa agatggtgag ttcttctcta tgctggggcc gtccggctcc ggcaaaacca
     8041 cctgcctgcg cctgattgct ggcttcgaac agctttccgg cggggctatc tctatctttg
     8101 gtaaacccgc cagcaatctg ccaccgtggg agcgggacgt gaatactgtc tttcaggact
     8161 acgcgctatt tccgcatatg tcgattcttg acaatgtcgc ctatgggctg atggtcaaag
     8221 gcgtgaataa aaagcagcgg cacgcaatgg cgcaagaggc gctggagaaa gtggcgttgg
     8281 ggtttgtaca gcaacgtaaa ccgtcacaac tttctggtgg tcagcgccag cgggttgcta
     8341 tcgccagagc attggtgaat gaaccgcgcg tattgctgtt ggatgaaccg ctcggcgcac
     8401 tggatctcaa attgcgtgag cagatgcagc tggaactgaa aaaactgcaa cagtctctcg
     8461 gtatcacttt tatcttcgtt acccacgatc agggcgaagc gttatcgatg tccgatcgtg
     8521 tggcggtttt caataatgga cgcattgagc aggtcgattc cccgcgcgat ctctatatgc
     8581 gcccgcgcac gccgtttgtt gccgggttcg ttggtacatc gaatgttttt gatggactga
     8641 tggcagagaa actttgtggc atgacgggaa gcttcgccct gcggccggaa catatccgcc
     8701 tcaacacccc tggtgaactg caggccaatg gcacgatcca ggcggtgcaa tatcagggcg
     8761 cggcaactcg ttttgaactg aaattgaacg gcggtgaaaa actgctggtg agtcaggcca
     8821 atatgacagg cgaagaactg cctgccacgc tcacgcccgg acaacaggtg ctggtttcct
     8881 ggtcgcgtga tgtgatggtg ccgctggttg aggagaggtg aatggcgatg aacgtattgc
     8941 aatcaccttc acgtccaggt ctgggtaagg tgtccggttt cttctggcgg aatccggggc
     9001 tggggctgtt tttgctgctg cttggcccgc taatgtggtt tggcattgtc tatttcggct
     9061 cgctgctaac gctgttatgg cagggctttt atacctttga cgatttcacc atgtcggtaa
     9121 cgccggaact gacgctggcg aacatccgtg cgctgtttaa tccggcgaat tacgacatca
     9181 ttctccgcac gctgactatg gctgtggcag tcactatcgc cagcgccatt ctggcttttc
     9241 caatagcgtg gtatatggcg cgctatacca gcgggaaaat gaaagcgttt ttttatattg
     9301 cggtaatgtt gccgatgtgg gcgagctaca ttgttaaagc ctatgcctgg acgttattgc
     9361 tggcaaaaga tggcgtggct cagtggtttt tacaacatct tgggctggaa ccactgctga
     9421 cagcgttcct tacattacct gcggtgggcg gcaatacgct gtcaacttcc gggctggggc
     9481 gctttctggt gtttctctat atctggctgc cgttcatgat cctgcccgtg caggcggcgc
     9541 ttgagcgttt gccgccgtca ttgttgcagg cgtcagctga tctcggcgca cgtccacgac
     9601 aaacctttcg ctatgtggtg ttgccgctgg cgatcccggg tattgccgct ggctctatct
     9661 ttaccttctc actcacactg ggcgatttta tcgtcccgca gctggttggc ccgccaggat
     9721 attttatcgg caatatggtt tattcccagc agggggcgat tggcaatatg ccgatggcgg
     9781 cggcattcac cctggtgccg attgttctta tcgcactgta cctggcgttc gtgaaacgtc
     9841 tgggagcgtt cgatgcactc tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc
     9901 ggcgttgttt tcctacattt tcccatcctg ataatcgccg cctatgcgtt taacactgaa
     9961 gatgcggcgt ttagttttcc accgcagggc ctgacgctgc gctggtttag cgtggcagca
    10021 cagcgtagtg atattcttga tgcagtgaca ctgtcactta aagtggcggc gctggcgaca
    10081 ttaatcgcgc tggtgttagg gacgctggca gctgccgcgc tgtggcgacg agactttttc
    10141 ggcaaaaacg ccatttcgct gttactgctg ctgcccattg cgctgccggg cattgtcact
    10201 ggtctggcgt tattaaccgc ctttaaaacc atcaatctgg agccgggatt tttcaccatc
    10261 gtggtcggtc atgcgacttt ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt
    10321 cgccgcacct catggagtct ggttgaggcg tcaatggatc ttggggccaa tggctggcaa
    10381 accttccgct acgtagtgtt gccgaatctc agttcggcgt tactggcagg aggaatgctg
    10441 gcgtttgcct tgtcgttcga tgaaatcatc gttacgacct ttacggcagg tcatgaacga
    10501 acgttaccgt tgtggttgct caatcagctt gggcgaccgc gtgatgtacc ggtaaccaac
    10561 gtggtggcac tgctggttat gttggtaaca accttgccga tcctgggggc ctggtggcta
    10621 acccgcgaag gcgacaatgg tcaataacca ctgatacagg aaaatgctat gcaacataag
    10681 ttactgatta acggagaact ggttagcggc gaaggggaaa aacagcctgt ctataatccg
    10741 gcaacggggg acgttttact ggagattgcc gaggcatccg cagagcaggt cgatgctgct
    10801 gtgcgcgcgg cagatgcagc atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg
    10861 gaatgtctgc tgaaactggc tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg
    10921 gagtcccgta attgtggcaa accgctgcat agtgcgttca atgatgaaat cccggcgatt
    10981 gtcgatgttt ttcgcttttt cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt
    11041 gaatatcttg aaggtcatac ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct
    11101 atcgcgccgt ggaattatcc gctgatgatg gcagcgtgga aactggctcc ggcgctggcg
    11161 gcagggaact gcgtagtgct taaaccatca gaaattaccc cgctgaccgc gttgaagttg
    11221 gcagagctgg cgaaagatat cttcccggca ggcgtgatta acgtactgtt tggcagaggc
    11281 aaaacggtgg gcgatccgct gaccggacat cctaaagtgc ggatggtgtc gctgacgggc
    11341 tctatcgcca ccggcgagca catcatcagc cataccgcgc cgtccattaa gcgtactcat
    11401 atggaacttg gtggcaaagc gccagtgatt gtttttgatg atgcggatat tgaagcagtg
    11461 gtcgaaggtg tacgtacatt tggctattac aatgccggac aggattgtac tgcggcttgt
    11521 cggatctacg cgcaaaaagg catttacgat acgctggtgg aaaaactggg tgctgcggtg
    11581 gcaacgttaa aatctggtgc gccggatgac gagtctacgg agcttggacc tttaagctcg
    11641 ctggcgcatc tcgaacgcgt cagcaaggca gtagaagagg cgaaagcgac agggcacatc
    11701 aaagtgatca ctggcggtga aaagcgcaag ggtaatggct attactatgc gccgacgctg
    11761 ctggctggcg cattacagga cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg
    11821 agtgttacgc tcttcgacaa cgaagaacag gtggtgaact gggcgaatga cagccagtac
    11881 ggacttgcat cttcggtatg gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg
    11941 ctgcaatatg gttgtacctg ggtcaatacc catttcatgc tggtaagtga aatgccgcac
    12001 ggtgggcaga aactttctgg ttacggcaag gatatgtcac tttatgggct ggaggattac
    12061 accgtcgtcc gccacgtcat ggttaaacat taaggataat attgcagatc gtaagagtat
    12121 gagatgatct tacgtactgt cagagcactg atttctctga cagtacgtgc tgcgtgcaaa
    12181 atcactacat caaataaacc agccaaatct catagagata agagtaaaca aaccaacagc
    12241 agcaaagaca tttatcagta ctacagttct actggaaaca ttcatttttg ccacctttta
    12301 tcattgcccc tatgtgtata gtacgggtta agaaaatccg caataatact cattctgaag
    12361 atggataagg gcaagttgct gtttgatgat tttttctcaa actggtgaaa aaacttgatg
    12421 cacgtcaaaa aatgacgcat atttgcgcgc gttttattca tctggctgga cgcccgtaca
    12481 tgtctctcta tcaacacatg cttgtcttct atgcggttat ggcagcaatc gcatttctta
    12541 tcacctggtt tctttctcac gataagaaac gcatccgttt cttaagcgcc tttctggtgg
    12601 gggcaacatg gccgatgagt ttcccggtgg cgctgttgtt ttcactgttt taaatcttac
    12661 tcactggctt gacggcacca cgcagactta tatcatttgg atgaatcgat aaatttcaca
    12721 agtggctaag gagaaagtat gtcgcatctg gatgaagtca tcgcgcgcgt ggatgccgcg
    12781 attgaagaga gcgtcattgc ccatatgaac gaattattaa tcgccctgag cgatgacgcg
    12841 gagttaagtc gggaagatcg ctacacccag cagcagcgtt tgcgcaccgc tattgctcat
    12901 cacggtcgca agcataaaga agatatggaa gcgcgccatg aacagttaac caaaggcggc
    12961 accatcctgt gattaaaatg agcgtctggc taccaaccag gcgccaacca caagtaaaat
    13021 agcgccacaa attgggccga acaacgcccg tagtggtacg ccatgactgc ggaagatatc
    13081 cagaaccaga ccgccaatca gctggctggc gaccagtact gcaatagttg tcgccgctcc
    13141 cacattctga tatccgctga tactggcgaa gacaaaaaac gatcccagca agccaggtat
    13201 aagtgtccac cagcgcacgc tggacaccag ttcgccaaac cccgccatac cttgtttaaa
    13261 ccataaaata gaaacaaaga gcacaatccc caccagtgag ttcagcaaca tggcgatgag
    13321 aatggtagag gaggtctggg tgatgcgcac catcagcgtg ttctggacta ccagaccaat
    13381 tccggcggca atcaaaaagg caagggtgag cgactgattc atccaatcgc gtccggttca
    13441 gtgcgttcgt cgagttgcaa ctgcataaat gtcagatcca gccagcgccc aaatttagtg
    13501 cccacctgcg gcatttgtgc ggtgacgaca aatcccagcg actggtgcag atgcagcgag
    13561 gcctgatttt gcgattcgat cccggcgacc atgacatgct tcccgcaatc tcgcgcttca
    13621 tcaatcaatc ggcttaacaa tttacgcccc agacctttgc cctgatgatc gggatggaca
    13681 taaaccgaat gttccacggt atggcgaaaa ccatcgaaac tacgccagtc gccaaatgag
    13741 gcatatcccg tcactacgcc atcttcttca cttaccagca ccggataacc tgctatggtc
    13801 cgcgcttcaa accaggcaat gcggttatct gcatccaccg tttggtcatt ccagatagcc
    13861 gccgtataca acacggcgtg gttataaatt tccgcaatgg cagcacagtc ggctttgcgg
    13921 gcaaaacgga tggacatgtc tggcctcaat aaaataatga tgatgatgtt atcaatacta
    13981 tcgaaaacag cccgaagcaa cggattcctc gatgaaatta ttgcgtgttg cagagggggt
    14041 gtgacggctg caaacaaaac caatcctgcg agcctgctac tattgatgga ttcatgccaa
    14101 aagcacagga gagcattatg gggcaacaaa agcagcgtaa tcgacgttgg gttctggcct
    14161 cgcgtccaca tggcgcaccg gttccggaga atttccgtct tgaagaagat gatgtcgcca
    14221 caccgggtga aggacaggtg ttactgcgca cagtttattt gtccctggac ccgtatatgc
    14281 gtggacgtat gagcgatgag ccatcttatt caccgccggt tgatattggc ggcgtgatgg
    14341 tcggcggtac ggtgagccgt gtcgaggagt cgaatcatcc tgattatcag tctggcgact
    14401 gggtgctggg ctacagtgga tggcaagact atgacatatc cagtggtgat gatctggtga
    14461 aacttggcga tcatccgcaa aatccatcgt ggtcgctggg tgtgctaggg atgccaggct
    14521 ttaccgctta tatgggccta ctggatatcg gtcagcctaa agagggcgaa acgttggtgg
    14581 tagctgcggc gacaggacca gtgggggcga cggtggggca aatcggcaaa cttaaaggtt
    14641 gcagagtggt gggggtagcc ggtggcgcgg aaaaatgccg ccatgctacc gaggtgttag
    14701 gcttcgatgt ttgccttgat caccacgcgg acgattttgc agaacaactg gcgaaagcgt
    14761 gcccaaaagg tattgatatc tattatgaaa acgtgggcgg taagatattt gatgcagtgc
    14821 taccattact taatacatct gcgcgcattc ccgtttgtgg cttagtgagc agctataacg
    14881 ctacagagct accacccggt ccggatcgtt tacccctgtt gatggcaacg gtgctgaaaa
    14941 aacgtattcg cttgcaaggg tttattatcg ctcaggatta tggtcaccgc atccatgagt
    15001 ttcagaagga gatggggcaa tgggtgaaag aggataaaat ccactaccgc gaagaaatta
    15061 ctgacggttt agagaatgcg ccacagacgt ttatcggcct gctgaagggt aaaaacttcg
    15121 gcaaagtggt gatccgcgtg gcgggtgatg attaaagttt caacggcggc gtaagccgcc
    15181 ataaaaatcc aggcaaaaag cttattttca gctttaatta actaaccaca tcattgttct
    15241 gctgctaata acaacaaagg tgagtggttt atgattattc actttaatac accaggtgaa
    15301 ttccttctgc catgcaggca gggttggaca gaaaacacgc gaaggaatta aaatgcctgg
    15361 aacggaaaaa atgaaacatg tcagtttgac tctgcaggtt gagaacgacc tgaaacatca
    15421 gcttagtatt ggcgcactaa aacctggcgc acgcctgatt actaaaaatc tggcggagca
    15481 attaggtatg agtattacac ctgtgcgtga agcattatta cgtctggttt cggtgaatgc
    15541 gctttctgtc gcaccagcgc aagcatttac agttccggaa gtggggaaac gtcaattaga
    15601 cgaaatcaat cggatccgct acgaactgga attaatggca gttgctctgg ctgttgaaaa
    15661 cctcaccccg caagacctgg cggaactcca ggaattgctc gagaaattac agcaggcgca
    15721 agaaaagggc gacatggaac aaatcattaa tgtaaacagg ctatttcgct tagcgattta
    15781 tcatcgctca aatatgccca tcctgtgtga gatgattgag caactgtggg tcaggatggg
    15841 acctggttta cattatctct atgaagcgat taatccagcg gaattacggg agcatataga
    15901 aaactatcat ctgttactcg ctgcgttaaa agcaaaagac aaagagggat gcagacattg
    15961 tcttgctgaa attatgcaac aaaatattgc tattttatac cagcaataca atcgttaaag
    16021 taaatgcccg gcaaataccg ggcatcaagg taattactca aatcgccatg cgatattcac
    16081 gccgacacca taatttcgtc cgggcgcagg ttcgtaatat cgcccgtttg actcattgac
    16141 aatgacagaa ccaacgtatt ctttatcgaa taaattatcg acacgaccaa ataaatccac
    16201 agttaaattg tggtaattgt atttataccc ggtgaataag ccgatgagag tataagacgg
    16261 cgccttggcc gtattttcat catctgccat aatatcgccc atataacgcg cttccgtgcc
    16321 tgcataccag ccatcttccg gtacataacc tatcgacgca aagcccatat tacgggcgat
    16381 ccctggcatc cgattaccgt tacaatcctg ttcattgcaa acattgctgc gataggtcgc
    16441 atcaagccag gtccaggacg cgtttacgcg aaaatctccc gcgaaacgtt gatcccatgc
    16501 cagttcagcg ccttgacgac gggtctttcc agcatttttg taagtcgtac gcccaccgct
    16561 actgctatcg acaacaattt catcatcggt gtcggtctgg aacaatgcga gactaagcag
    16621 cccatcacca atacgcgttt tactgccgat ctcaattgta tcgttggtgg atggttttaa
    16681 accaaagttc ataccgcttt gcccatcagc gcgataagac agctcattaa tcgtcggcgt
    16741 ttcaaaacca cgcccggctg ccagatagat attccaggca tcggtcattg catattttaa
    16801 cgatccggct ggtagccatt tgtgataact ggcatcaccg ctgtcatcgc cgttacccgg
    16861 agtaacgtaa tggtcgttgg aatcaaacca cacggagctg tagcgcacgc cagcatccag
    16921 cgacagtttt tcgctcagct gccactgcgt ctgtaaatag ggatcgatgt tccacatcag
    16981 attgcgttcg tcgcgacgca actcaccttt ttgcccgtac tccggcatgc cgctattcag
    17041 gcggaagtta ttgtagccct tgcggttttc actcatgttt tcgtagttca ggccggtagt
    17101 gaacgtgacc ggaacgccca gttcaccacg gtgtgtccag cggctgtcta ttccctggta
    17161 atggcgttgc agggtaatca cgccgcccgc atgtgacggg ttaagttgtg gcgccatggg
    17221 tattgactgg tactgggtcg tttctcgctc tccggcatac atcatcacac tcatatcatc
    17281 ccgcgaactc aggctacgtt catagcgcaa cccagcctga gtttgcttga tggtttttcg
    17341 cgtgtcgtac tgttctgcac gaggcgcttg ttgtggattc gccttccatt ctgctttggt
    17401 tagcccaccc gggtcatctg ctttgatatc cacactattg aaaatcaggc ttaatttgct
    17461 ggcttcatca atgcgtacgc ccagtttggc attggctaaa tttttctgtg cgccactatg
    17521 gtcacgatag ccgtgggtcg taaaacgcgt ggttgagacg gtgtaatcaa catcgccggg
    17581 ctgtgtgccg tctcccgttg cgcccgttgc tttcagccca tagcgccagc tgccaaaact
    17641 gccgtagtaa ctactggctt caatggttgg tggctgttgt ccggtctggg tggtgacatt
    17701 cattacaccg ccagacgcgt tgccatacag ggcagagaag gggccacgca gcacttccac
    17761 attttgcaca ctgcttaaat cgatgttgga tgtttgccct tgcccgtcgg gcatggtggc
    17821 gggaataccg tccacataca ggcgaatacc gcgaattccg taagtggaac gggagccaaa
    17881 tccgcgaatc gacagctgta aatcttgcgc atagttctgc cggttttgta cctgcaaacc
    17941 aggcacgccg gtcagtgatt cggacaagtt aatgcgcggt gttgccaggc gcatctcctc
    18001 gccatccacc acgcttactg ctgcaggggt atccagttct gaaaccacct gcggcgcggc
    18061 actgacaatc atggtctgtt catcagcggc aaaaacaacg ggggaaagga caagcagtgc
    18121 gggcaaaacg gtctgtcgga cggaaaaaat cttcatgaaa aaagccaggt taagaatggg
    18181 aaaacgccgt catggtaatg aaattgtaaa tttatggaaa atgaaacggc acaatacgtt
    18241 aagtaattga gaaaattgta gtcgtaacgg caagaaatgc tccacatttg agaaaataat
    18301 gattaccatt cccatttata acaagagcgt aacgatgatt acgcttagcg aagcattgtg
    18361 aagcagcaaa aatatcggtt catcaaaggg agtcgtcatg catttacgtc atctgttttc
    18421 atcgcgcctg cgtggttcat tactgttagg ttcattgctt gttgcttcat cattcagtac
    18481 gcaggccgca gaagaaatgc tgcgtaaagc ggtaggtaaa ggtgcctacg aaatggctta
    18541 tagccagcaa gaaaacgcgc tgtggctcgc cacttcgcaa agccgcaaac tggataaagg
    18601 tggcgtggtt tatcgtcttg atccggtcac tctggaagtg acgcaggcga tccataacga
    18661 tctcaagccg tttggtgcca ccatcaataa cacgactcag acgttgtggt ttggtaacac
    18721 cgtaaacagc gcggtcacgg cgatagatgc caaaacgggc gaagtgaaag gccgtctggt
    18781 gcttgatgat cgtaagcgca cggaagaggt gcgcccgctg caaccgcgtg agctggtagc
    18841 tgatgatgcc acgaacaccg tttacatcag tgttattggt aaagatagcg tgatttgggt
    18901 cgttgatggc gagaatatca aactgaaaac cgccatccag aacaccggta aaatgagtac
    18961 cggtctggcg ctggatagca aaggcaaacg tctttacacc actaacgctg acggcgaatt
    19021 gattaccatc gacaccgccg acaataaaat cctcagccgt aaaaagctgc tggatgacgg
    19081 caaagagcac ttctttatca acattagcct tgataccgcc aggcagcgtg catttatcac
    19141 cgattctaaa gccgcagaag tgttagtggt cgatacccgt aatggcaata ttctggcgaa
    19201 ggttgcggca ccggaatcac tggctgtgct gtttaaccca gcgcgtaatg aagcctacgt
    19261 aacgcatcgt caggcaggta aagtcagtgt gattgacgcg aaaagctata aagtggtgaa
    19321 aacgttcgat acgccgactc atccaaacag cctggcgctg tctgccgatg gcaaaacgct
    19381 gtatgtcagt gtgaaacaaa aatccactaa acagcaggaa gctacccagc cagacgatgt
    19441 gattcgtatt gcgctgtaat agatatggcg agggtgaaaa cggagagact tgcctgactt
    19501 ttatcacgcc gcatgcggca caccttcgtc agtaatctaa atttgcccgg cacgtagccg
    19561 ggcaattttg caattagctc gccgtttcag gcttaaacac aatttcctgt ttttcttcat
    19621 cttcctcgac gactggcgca gtgctgtgaa tttcagcaac gcgtttgcgc acaccaaacc
    19681 agcctataac cagcagaata ccgataatcg gcagcgccgc gatagtataa gtcccgttcg
    19741 ggtaatcgaa cgccatcagc acaaggacac tcagtaaaaa cagtaatgtc agccaggaag
    19801 tgaagggcgc gccaggcagt ttaaaactga catccgctgc tttgccttct ttaatcgctt
    19861 tacgcaggcg catctggcac acgatgataa acgcccatga agcgatgatt cccagcgacg
    19921 cgaagttcaa cacaatctca aatacgcgcg acggcaccag atagttgagg aatacgccga
    19981 cgacatacac aactagtgtc gccagaatcc cggcatacgg cacatgctga cgactcattt
    20041 tcgccataaa actcggtgcg gaaccgccca tcgccattga gcgcagaata cgtccggtgc
    20101 agtacagacc tgaattcagg ctggagaggg cagcggtcag caccacaatg ttcataatgc
    20161 tgccgatata tggcacaccc agtttagaga aaaacgtcac gaacggactt tgccccgcct
    20221 gatacgcgct ccacggcaat aacataacca gcaacaccac ggagccgacg taaaacaggc
    20281 caatacgcca aatcacactg ttaatcgctt taggcaccat ggtctgcgga tctttacatt
    20341 ctccggcagc tgtacccacc atttcaatgg aggcaaaagc aaacactacg ccctgaatca
    20401 acaccagcgc aggcagcaaa ccgtggggga agaagccgcc attatcggtg attaaatgaa
    20461 agccagtggt gttgccatcc agcggctgac cactaccgag gaacactgta cccacgacca
    20521 aaaaggtcac aatggcgagc actttaataa gcgcaaacca gaactccatc tccgcaaacc
    20581 atttcacacc gatcatattc atggtgccaa cgatggtaag tgcagcgagc gcaaagaccc
    20641 actgcggcac gccgccaaac gcaccccagt aatgcatata cagagcgacg gcggtaatat
    20701 caacaatccc ggtcatcgcc cagttgatga agtacatcca accggcaaca taagcggctt
    20761 tctcacccag aaactcacgg gcataggaga caaagctgcc actggaaggg cgatgcaata
    20821 ccagctcacc caacgcgcgc agaataaaaa acgaaaacag gccgcagatt aaatagacca
    20881 gtgccagcgc cggccccgcc atttgcagtc gggctcccgc gcctaaaaac aaaccagtac
    20941 caatcgcacc gccaatggcg atcatctgca cctgacgatt gcccattgct ttgtgatatc
    21001 cctcttcgtg ggcattaagc cagcggcgtt tcgcagcgtg ttgctctgaa gtgtcggtgt
    21061 tgtgtttact cattgcgctc cctgattgct ttaatgaaaa aagtctttta tgtcgccatg
    21121 aacaatgttt attcatcctg gcgatgatag ttatcaccat tattctttat gggtataaaa
    21181 ctatcggcgc agcatcctac ccgttatcga taaacgatgc aaaacatccc cttacaatcc
    21241 tgaaggggat taatacaact gacgaaaaaa tgacaaatcc ttttgctggt taacctgtgt
    21301 actgtcctac acttaatctt taaaagattg tgaggggcat atgattaagg tgtatggcgt
    21361 acccggatgg ggctctacaa tcagtgagct gatgctaaca ctggctgata ttccttatca
    21421 gttcgttgat gtcagtggtt ttgaccacga gggagcttca cgcgagttat tgaaaaccct
    21481 gaacccgttg tgtcaggttc cgacactggc attggaaaat gacgagatta tgacggagac
    21541 agcggcgatt gcattgatgg ttctcgatcg tcgcccggac cttgcgccac cggttgggcg
    21601 cgccgaacgt cagcagtttc aacggctatt ggtctggctg gttgctaatg tctatccaac
    21661 attcactttc gccgattacc ctgagcgttg ggctcctgac gcacccgaac agttaaagaa
    21721 gaatgttatt gaatatcgta aatcgcttta tatctggctg aattcacaat tgaccgctga
    21781 accttatgcg tttggtgagc aactaacgct ggtggactgt tatctttgca ctatgcgcac
    21841 atgggggcct gggcatgaat ggtttcagga taatgccacg aatatcagtg cgattgctga
    21901 tgctgtatgt c
//
LOCUS       DAZKFU020000062        21576 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZKFU020000062 DAZKFU020000000
VERSION     DAZKFU020000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.062"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            155..1669
                     /gene="caiT"
                     /locus_tag="ACHOQQ_RS09190"
                     /old_locus_tag="pgaptmp_001838"
     CDS             155..1669
                     /gene="caiT"
                     /locus_tag="ACHOQQ_RS09190"
                     /old_locus_tag="pgaptmp_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGU4470102.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1700..2842
                     /gene="caiA"
                     /locus_tag="ACHOQQ_RS09195"
                     /old_locus_tag="pgaptmp_001839"
     CDS             1700..2842
                     /gene="caiA"
                     /locus_tag="ACHOQQ_RS09195"
                     /old_locus_tag="pgaptmp_001839"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGU4470103.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2971..4188
                     /gene="caiB"
                     /locus_tag="ACHOQQ_RS09200"
                     /old_locus_tag="pgaptmp_001840"
     CDS             2971..4188
                     /gene="caiB"
                     /locus_tag="ACHOQQ_RS09200"
                     /old_locus_tag="pgaptmp_001840"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGU4470104.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4262..5815
                     /gene="caiC"
                     /locus_tag="ACHOQQ_RS09205"
                     /old_locus_tag="pgaptmp_001841"
     CDS             4262..5815
                     /gene="caiC"
                     /locus_tag="ACHOQQ_RS09205"
                     /old_locus_tag="pgaptmp_001841"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGU4470105.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPKFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5924..6709
                     /gene="caiD"
                     /locus_tag="ACHOQQ_RS09210"
                     /old_locus_tag="pgaptmp_001842"
     CDS             5924..6709
                     /gene="caiD"
                     /locus_tag="ACHOQQ_RS09210"
                     /old_locus_tag="pgaptmp_001842"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGU4470106.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVDSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6715..7305
                     /gene="caiE"
                     /locus_tag="ACHOQQ_RS09215"
                     /old_locus_tag="pgaptmp_001843"
     CDS             6715..7305
                     /gene="caiE"
                     /locus_tag="ACHOQQ_RS09215"
                     /old_locus_tag="pgaptmp_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGU4470107.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGHDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRTVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVAPKR"
     gene            complement(7424..7819)
                     /gene="caiF"
                     /locus_tag="ACHOQQ_RS09220"
                     /old_locus_tag="pgaptmp_001844"
     CDS             complement(7424..7819)
                     /gene="caiF"
                     /locus_tag="ACHOQQ_RS09220"
                     /old_locus_tag="pgaptmp_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGU4470108.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8080..11301)
                     /gene="carB"
                     /locus_tag="ACHOQQ_RS09225"
                     /old_locus_tag="pgaptmp_001845"
     CDS             complement(8080..11301)
                     /gene="carB"
                     /locus_tag="ACHOQQ_RS09225"
                     /old_locus_tag="pgaptmp_001845"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGU4470109.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11319..12467)
                     /gene="carA"
                     /locus_tag="ACHOQQ_RS09230"
                     /old_locus_tag="pgaptmp_001846"
     CDS             complement(11319..12467)
                     /gene="carA"
                     /locus_tag="ACHOQQ_RS09230"
                     /old_locus_tag="pgaptmp_001846"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGU4470110.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETAIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12923..13744)
                     /gene="dapB"
                     /locus_tag="ACHOQQ_RS09235"
                     /old_locus_tag="pgaptmp_001847"
     CDS             complement(12923..13744)
                     /gene="dapB"
                     /locus_tag="ACHOQQ_RS09235"
                     /old_locus_tag="pgaptmp_001847"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGU4470111.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGSIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLHNL"
     gene            complement(13911..14825)
                     /gene="rihC"
                     /locus_tag="ACHOQQ_RS09240"
                     /old_locus_tag="pgaptmp_001848"
     CDS             complement(13911..14825)
                     /gene="rihC"
                     /locus_tag="ACHOQQ_RS09240"
                     /old_locus_tag="pgaptmp_001848"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGU4470112.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAVRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVEAQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALAP"
     gene            complement(14891..15841)
                     /gene="ispH"
                     /locus_tag="ACHOQQ_RS09245"
                     /old_locus_tag="pgaptmp_001849"
                     /gene_synonym="lytB"
     CDS             complement(14891..15841)
                     /gene="ispH"
                     /locus_tag="ACHOQQ_RS09245"
                     /old_locus_tag="pgaptmp_001849"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGU4470113.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15843..16292)
                     /gene="fkpB"
                     /locus_tag="ACHOQQ_RS09250"
                     /old_locus_tag="pgaptmp_001850"
     CDS             complement(15843..16292)
                     /gene="fkpB"
                     /locus_tag="ACHOQQ_RS09250"
                     /old_locus_tag="pgaptmp_001850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4470114.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16380..16874)
                     /gene="lspA"
                     /locus_tag="ACHOQQ_RS09255"
                     /old_locus_tag="pgaptmp_001851"
     CDS             complement(16380..16874)
                     /gene="lspA"
                     /locus_tag="ACHOQQ_RS09255"
                     /old_locus_tag="pgaptmp_001851"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGU4470115.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16874..19690)
                     /gene="ileS"
                     /locus_tag="ACHOQQ_RS09260"
                     /old_locus_tag="pgaptmp_001852"
     CDS             complement(16874..19690)
                     /gene="ileS"
                     /locus_tag="ACHOQQ_RS09260"
                     /old_locus_tag="pgaptmp_001852"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGU4470116.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19733..20674)
                     /gene="ribF"
                     /locus_tag="ACHOQQ_RS09265"
                     /old_locus_tag="pgaptmp_001853"
     CDS             complement(19733..20674)
                     /gene="ribF"
                     /locus_tag="ACHOQQ_RS09265"
                     /old_locus_tag="pgaptmp_001853"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGU4470117.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20682..20900)
                     /gene="yaaY"
                     /locus_tag="ACHOQQ_RS09270"
                     /old_locus_tag="pgaptmp_001854"
     CDS             complement(20682..20900)
                     /gene="yaaY"
                     /locus_tag="ACHOQQ_RS09270"
                     /old_locus_tag="pgaptmp_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGU4470118.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            21003..21266
                     /gene="rpsT"
                     /locus_tag="ACHOQQ_RS09275"
                     /old_locus_tag="pgaptmp_001855"
     CDS             21003..21266
                     /gene="rpsT"
                     /locus_tag="ACHOQQ_RS09275"
                     /old_locus_tag="pgaptmp_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGU4470119.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5133 a         5806 c         5562 g         5075 t
ORIGIN      
        1 gtgacactaa tcacagaata cagcttattg aatacccatt atgagttagc cattaacgcg
       61 tccacgaggt taataataat tatattaaat gttaacaaaa ataaaacaaa cgggaacgca
      121 aaataaatat tcgttttcac agtggaatta actcatgaag aatgaaaaga gaaaaacggg
      181 aatagaaccg aaggttttct ttccaccgtt aataatcgtc ggcatacttt gttggcttac
      241 agtcagagat ctggatgcgg cgaatgtcgt tattaatgct gtattcagtt acgtcaccaa
      301 tgtatgggga tgggcatttg aatggtatat ggtggtgatg cttttcggtt ggttctggct
      361 ggtgtttggc ccgtatgcca aaaagcgttt aggtaacgaa ccgccagaat ttagcaccgc
      421 cagttggatc tttatgatgt tcgcctcctg tacgtctgct gccgtactgt tctggggatc
      481 gattgagatc tactactaca tctccacccc gccgtttggc ttagaaccga actcgacagg
      541 agcgaaagag ttggggctgg cttacagctt gttccactgg ggacctctgc cgtgggccac
      601 ttacagcttc ctttcagtcg ccttcgctta cttcttcttt gtccgcaaaa tggaagtgat
      661 tcgccccagc tcgacactgg tgccgctggt aggtgaaaaa cacgccaaag ggttgttcgg
      721 taccatcgtc gacaacttct atctcgtcgc cttgatcttc gcgatgggta ccagtctggg
      781 ccttgccacg ccgctggtga ccgagtgtat gcaatggttg tttggcattc cgcataccct
      841 gcaactggac gctatcatca ttacctgctg gattatcctc aacgccattt gcgtcgcctg
      901 cggtctgcaa aaaggggtac gtatcgccag tgacgtgcgt agttacctga gcttcctgat
      961 gctgggttgg gtgttcattg tcagcggtgc cagcttcatc atgaactact tcaccgattc
     1021 ggtggggatg ttgctgatgt atctgccgcg aatgttgttc tataccgatc ccatcgctaa
     1081 aggcggcttc ccgcagggct ggaccgtgtt ctactgggca tggtgggtga tttacgccat
     1141 ccagatgagt atcttcctcg cccgcatctc ccgtggtcgt actgtgcgtg aactgtgctt
     1201 cggcatggtg atggggctga cagcatccac ctggatcctg tggaccgtgc tcggtagtaa
     1261 cactctgctg ttgatggata aaaacatcat caacattcca aatctgatcg aacagtacgg
     1321 tgtggcgcgc gccatcatcg aaacctgggc cgctctgcca ctcagcaccg ccaccatgtg
     1381 gggcttcttc atcctctgct ttattgccac cgttacgctg gttaacgcct gctcttatac
     1441 cctggcgatg tctacttgcc gcgaagtacg cgatggtgaa gaaccaccgc tgctggtgcg
     1501 tatcggttgg tcaattctgg ttggcattat cggtattgtt ctgctggcgc tcggcggcct
     1561 gaaaccgatt caaaccgcca ttatcgccgg aggatgcccg ctgttcttcg tcaacattat
     1621 ggtgacgctc tcctttatta aagacgcgaa acagaactgg aaagattaat taacccccaa
     1681 aatatcaaga ggttgaaaga tggattttaa tttaaatgat gagcaggaac tgtttgtcgc
     1741 cggtatccgc gaactgatgg ccagcgaaaa ctgggaggcc tattttgccg agtgcgaccg
     1801 tgacagcgtc tacccggaac gttttgtcaa agcactggcg gatatgggta tcgacagtct
     1861 gctgatccct gaagagcacg gtggtctgga cgcggggttt gttactctcg ccgccgtgtg
     1921 gatggagctg ggacgtctgg gggcaccaac ctatgtactg taccagttgc cgggcggatt
     1981 caacaccttc ctgcgcgaag gcacacaaga gcagatcgac aagattatgg ctttccgcgg
     2041 caccggtaag cagatgtgga actcagcgat taccgaaccg ggcgcgggct ccgacgtggg
     2101 tagcctgaaa acgacttata cccgtagaaa tggtaagatt tatcttaatg gtagtaagtg
     2161 ttttattacc agcagcgcct acaccccgta catcgtggtg atggcgcgcg acggggcttc
     2221 tccggacaaa cctgtctaca ccgaatggtt tgttgatatg agcaaaccgg gcatcaaagt
     2281 gaccaaactt gagaagctcg gtctgcgtat ggatagctgc tgtgaaatca cctttgacga
     2341 tgtggaactg gacgagaaag acatgttcgg tcgggaaggt aacggcttta accgcgtcaa
     2401 agaagagttc gaccatgaac gtttcctggt agccctgacc aactacggta cggcgatgtg
     2461 cgcctttgaa gatgcggcgc gctacgccaa ccagcgcgtg cagtttggcg aggctattgg
     2521 tcgtttccag ttgattcagg aaaaattcgc ccacatggcg atcaaattaa actccatgaa
     2581 aaacatgctg tatgaagcag cgtggaaagc agacaacggc accatcacct ctggcgatgc
     2641 agcgatgtgc aaatacttct gcgccaatgc ggcatttgaa gtggtggata gcgcaatgca
     2701 ggtgctgggc ggtgtcggga ttgcgggcaa ccaccgcatc agccgcttct ggcgtgacct
     2761 gcgtgtagac cgcgtttccg gaggatctga cgaaatgcag atcctgacgc tgggtcgtgc
     2821 ggtgctgaag caataccgct aagtccgtgc gctgcctgat gcgacgctga cgcgtcttat
     2881 caggcctacg ggagcacgaa tgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
     2941 tgcgctacat aaatttttca ggagctaata atggatcatc tacccatgcc gaaattcgga
     3001 ccgctggccg gattgcgcgt tgtcttctcc ggtatcgaaa tcgccggacc gtttgccgga
     3061 caaatgttcg cagaatgggg cgcggaagtt atttggatcg agaacgtcgc ctgggccgac
     3121 accattcgcg ttcaaccgaa ctacccgcag ctctcccgcc gcaatttgca cgcgctgtcg
     3181 ttaaatattt tcaaagatga aggccgcgaa gcgtttctga aattaatgga aaccaccgat
     3241 atcttcatcg aagccagtaa aggtccggcc tttgcccgtc gtggcattac cgatgaagta
     3301 ctgtggcagc acaacccgaa actggttatc gctcacctgt ccggttttgg tcagtacggc
     3361 accgaggagt acaccaatct tccggcctat aacaccatcg cccaggcctt cagtggttac
     3421 ctgattcaga acggtgatgt tgaccagcca atgcctgcct tcccgtatac cgccgattac
     3481 ttttctggcc tgaccgccac cacggcggcg ctggcagcac tgcataaagc gcgtgaaacc
     3541 ggtaaaggcg aaagtatcga catcgccatg tatgaagtga tgctgcgtat gggccagtac
     3601 ttcatgatgg attacttcaa cggcggcgaa atgtgcccgc gcatgagcaa aggtaaagat
     3661 ccctactacg ccggttgcgg tctgtataaa tgcgccgacg gctacatcgt gatggaactg
     3721 gtgggcataa cccaaattga agagtgcttt aaagatattg gcctcgcaca tctactcggt
     3781 acaccggaaa tcccggaagg cactcagctt atccaccgta tcgaatgccc ttacggccca
     3841 ctggtagaag aaaaactcga tgcctggctg gcggcacata ccatcgcaga agttaaagaa
     3901 cgctttgccg aactgaatat cgcctgtgcc aaagtactga ccgtaccgga actggaaagc
     3961 aatccacagt atgttgcccg tgaatcaatc actcagtggc aaacgatgga tggtcgcacc
     4021 tgcaaagggc cgaacatcat gccgaaattc aaaaataacc ccggacaaat ctggcgcgga
     4081 atgccctcac atggcatgga cacggctgcc attttgaaaa atatcggcta cagcgaaaac
     4141 gacattcagg agttggtcag caaaggtctg gccaaagttg aggactaaac gcgtggcgtc
     4201 agtcggcttg cctgactagc gcccgcccaa taacaaacga acaaaaatgg atagaggtgc
     4261 aatggatatc attggcggac aacatctacg tcaaatgtgg gacgatctgg cggacgttta
     4321 cggtcataaa acggcgctga tttgtgaatc cagcggcgga gtcgttaacc ggtatagtta
     4381 tcttgagtta aatcaggaga ttaaccgcac ggcaaacctg ttttatacgc tggggattcg
     4441 caaaggcgac aaggttgcac tacatctcga caactgcccg aaatttatct tttgctggtt
     4501 cgggctggca aaaattggcg cgattatggt gccgattaac gcccgcctgt tacgcgagga
     4561 aagcgcgtgg atcctgcaaa atagccaggc gtgcctgctg gtgacaagtg cgcaattcta
     4621 tcctatgtat caacagattc agcaggaaga tgccactcaa ttgcggcaca tttgcctgac
     4681 agatgtggca cttcccgctg atgatggcgt gagttcgttt actcaactga aaaatcaaca
     4741 acctgccacc ttgtgctatg caccgccgct atcgactgac gatacggcgg aaattctctt
     4801 cacctccggc accacctccc gaccgaaagg tgtggtgatt acccattaca acctgcgctt
     4861 cgctggatat tactccgcct ggcagtgtgc actgcgtgac gatgacgtct acctgacggt
     4921 aatgcctgcg tttcatatcg attgccagtg tactgcggcg atggcggcgt tttctgccgg
     4981 ggccaccttt gtgctggtcg agaaatacag cgcccgcgcc ttctggggac aggtgcagaa
     5041 gtaccgcgcc accattaccg aatgtattcc gatgatgatt cgtacgttga tggtacagcc
     5101 gccttcagcg aacgatcggc aacaccgcct gcgggaagtg atgttttatc tcaacttgtc
     5161 ggagcaggaa aaagatgcgt tttgtgaacg cttcggcgtt cgcttgctga cgtcttatgg
     5221 gatgacggaa accattgtgg gcattatcgg cgatcgccct ggcgataaac gacgctggcc
     5281 gtcgattggt cgggcggggt tttgctacga agcggagatc cgcgacgatc acaatcgccc
     5341 gctcccggca ggtgagatcg gtgaaatctg tattaaaggc gtaccaggga aaaccatctt
     5401 caaagagtat tttctcaacc cgaaagccac tgcaaaagtg ctggaagctg atggctggct
     5461 gcataccggc gataccggat accgcgacga agaaggcttt ttttatttcg tcgatcgccg
     5521 ctgcaatatg atcaaacgcg gtggcgagaa tgtctcctgc gtggagctgg aaaatatcat
     5581 cgccacccat ccgaaaattc aggacatcgt ggttgtgggt attaaagatt cgattcgcga
     5641 tgaagccatc aaagcatttg tggtgctgaa tgaaggtgaa acattgagcg aagaggaatt
     5701 ttttcgcttc tgcgaacaaa atatggcgaa atttaaagtg ccctcttatc tggagatcag
     5761 aaaagatctg ccacgtaatt gctcggggaa aataattaga aagaatctga aataaatgaa
     5821 acggcaggga actaccctgc cagctaataa tcacgctcta aaacaatatg cattttttgc
     5881 agggatgttg tcgtccctga aaaagcaaaa atggagaaaa ggaatgagtg aatcattaca
     5941 tctgacccgc aatggatcaa ttctggaaat tacccttgat cgcccaaaag cgaatgctat
     6001 tgatgcaaaa accagctttg aaatgggcga agtatttcta aatttccgtg acgatccgca
     6061 attacgtgtc gccattatta ccggtgccgg agagaaattc ttttccgcgg gctgggattt
     6121 aaaagcggca gcagaaggcg aagcaccgga tgctgacttt ggcccgggtg gttttgcggg
     6181 attaaccgaa attttcaatc tcgacaaacc ggttatcgca gctgtgaacg gctacgcctt
     6241 tggcggcggt ttcgaactgg cgctggcggc agattttatt gtttgtgccg ataacgccag
     6301 cttcgccctg ccggaagcca aactgggcat cgttcctgac agcggcggcg tgctgcgtct
     6361 gccgaagatc ctgccgcctg ccatcgtcaa tgaaatggtg atgaccggca gacgaatggg
     6421 cgcagaagag gcgctgcgtt ggggagtagt caaccgcgtg gttagccagg cggaactgat
     6481 ggataacgcc cgcgaactgg cgcagcaact ggttgacagc gccccgctgg cgatcgcagc
     6541 gctgaaagag atctaccgca ccaccagcga aatgccggta gaagaagcgt atcgctatat
     6601 tcgcagcggc gtgttgaaac actatccatc agttctgcat tcagaagatg ccattgaagg
     6661 gccgctggcg tttgccgaga agcgcgatcc ggtgtggaaa ggacgttaac aaccgtgagc
     6721 tattacgcct ttgaggggtt aattccggtg gttcacccga cggcgtttgt ccatcccagt
     6781 gccgtcttga ttggcgatgt gattgtggga gccggtgtct acatcggccc actcgcctca
     6841 ctgcgtggtg actacgggcg gttgatcgtg caagcgggag ccaatattca ggatggctgc
     6901 attatgcatg gctactgcga cactgacact atcgttgggg aaaacggcca tatcgggcac
     6961 ggagcgatcc tgcatggttg tgtgattggt cacgatgcat tggtcgggat gaacagcgtg
     7021 attatggatg gtgcggtcat tggcgaagag agcattgttg ccgccatgag ctttgtcaaa
     7081 gcgggctttc gcggcgagaa acgtcagttg ttgatgggta cgccagcccg cgcggtacgc
     7141 actgttagtg acgacgagtt acactggaaa cggttgaata ccaaagagta tcaggatctt
     7201 gtcgggcgct gtcatgcatc gttacatgaa acacagccac tgaggcaaat ggaggaaaat
     7261 cgcccccgtt tgcaggggac gacggatgtg gcgccgaaac ggtaatcttg ccggatgcgg
     7321 cgtaaacgcc ttatccaact ctgctggaga gcaaccgtag gccggataag atgcgccagc
     7381 atcgcatccg gcgaccaggt cgcacggtat catcgaaatg acattaacga cgcatactct
     7441 ttgacaacat catctgccac ttctgctcgc ggttaagttc cgtgagcgga acggcaggaa
     7501 tacgcggtgt ctttcttacg ccgactaatt tttcccgact gttattgcgc aggcgcgcgt
     7561 aaatttgctc atcgatatcg accactttaa ccagtcgctg acactggcat ccccgccctt
     7621 ccagcttatt agggatcatc ttaacttcgc agcttatttc tgtgacttcc gacagaatat
     7681 aagtcagggt attaaccgcc ttgctgtgtt caatatcgaa atgaatagag atctctcttg
     7741 ctatcaccca ccgattttca gccatcatcc attcggcgat taacaagtag agtggttttt
     7801 caacatatcc ttcacacata cgaactccag tttcacaaat taaaagtctg gctatgactc
     7861 gttttgaagc aattaaacaa ggccatatta aaattgtgga tatatcgata gtatgcaact
     7921 tcaccgtcga taagtgtgag ctaaatcctg cttatgtcat caaaaatatc gattcccgaa
     7981 aagatttact taatgggcaa aaaaaacggt cctcatcaga gaaccgtatt agttattcga
     8041 tcaaattagc ggatgaaaaa tatccgccat gacacgctat tatttgatct gcgcgtgcat
     8101 ttcctgcacc gaaattactt tttcggtcgc atcggcattc agcgccatcg cggtggcaaa
     8161 gccgccgttc agggtggtgt cgtaatgcac tttatattgc agcgcactgc gacggatcac
     8221 gcgggagtct tcaatcgcgc gacgacctga agtggtgttg atgatgtagg tatattcgcc
     8281 attcttgatg cggtcctgaa tgtgcggacg accttcatgc accttgttta ccagacgcgg
     8341 attgataccc gcttcgccca gcacaatcgc cgtgccgtgg gtcgcatcca gctcgaagcc
     8401 ctgtttcagc agttttgccg ccaggtccac cacgcgttct ttatcgcctt cgcgcacgga
     8461 aagcagcgca cgaccgtgtt tcttcatggt ggagttgctg cccagctgcg ctttggcaaa
     8521 cgcttcagcg aaggtgcggc ccacgcccat gacttccccg gtagagcgca tttctggccc
     8581 taacagcggg tcaacgcccg ggaatttatt gaacggcagc accacttctt tcaccgagta
     8641 gtacggcggg ataacttctt tggttacgcc ctgctcagcc agcgatttgc cagccatcac
     8701 acgcgccgcc acttttgcca gcggtacgcc ggtggctttg gagacgaacg gaacggtacg
     8761 cgccgcacgc gggttaactt caatcaggta gacttcgttg tttttcaccg caaactgcac
     8821 gttcatcagg ccgcgcacct gcaattcgaa ggccagtttc tgcacctgct ggcgcatcac
     8881 atcctgaatt tcctgactta aggtgtacgc tggcagcgaa catgcggagt caccggagtg
     8941 cacgcccgcc tgctcgatgt gctccatgat gccgccaatc agcaccattt cgccgtcgca
     9001 gatggcatcc acgtcaactt ctactgcgtc atcaaggaaa tgatccagca acactggcgc
     9061 atcgttagac acgctaaccg ccgtctggaa gtagcgacgc aggtcagctt cgtcatagac
     9121 gatttccatc gcccgaccgc cgagaacgta agacggacgc accaccagcg ggtagccaat
     9181 ctcttttgcc ttctcaactg ccatttcaat agcagtaacg gtggcgttcg ccggttgttt
     9241 cagtttcaga cggtcaaccg catgctggaa gcgttcacgg tcttccgcac ggtcgatagc
     9301 atccgggctg gtgccgataa ccggtacgcc agcggcttcc agtgcacgcg ccagtttcag
     9361 cggggtctga ccgccgtact gaacgataac gcctttcggc ttctcaatac gcacgatttc
     9421 cagcacatct tccagagtta ccggctcgaa gtagaggcga tcggaagtgt cgtagtcggt
     9481 agagacggtt tccgggttac agttaaccat aatggtttcg taaccgtctt cacgcagcgc
     9541 cagcgaggcg tgtacgcagc agtagtcgaa ttcgataccc tgaccgatac ggtttggacc
     9601 gccgccaagc accatgattt tttcacggtc ggtagacgga ttcgcttcgc actcttcttc
     9661 ataagtggag tacatgtaag cggtgtcggt ggcaaactct gccgcacagg tatccacgcg
     9721 cttgtagacc gggtgcaggt catactggtc acgcagctta cggatttccg cttcgcgtac
     9781 gcccgccagt tttgccaagc gcgcatcggc aaagcctttg cgtttcagct ggcgcaggaa
     9841 ttcagcgttc aggccagtga tgcccacttc cgccactttc tcttccagac gcaccagttc
     9901 ttcaatctgt accaggaacc agcggtcaat gttagtcagg ttgaagacgc cgtccacaga
     9961 caggcccgcg cggaaagcat cggcgatgta ccagatacgc tctgcgcctg cgtctttcag
    10021 ttcgcgacgg attttggtta acgcttccgg gtcatccagg ctcactttcg ggtcgaatcc
    10081 agtcgcaccg acttccaggc cgcgcagcgc tttttgcagg gattcctgct gcgtgcgacc
    10141 aatcgccatc acttcgccaa ccgatttcat ctgagtggtc agacggtcgt tagcaccggc
    10201 gaatttttcg aagttgaagc gaggaatttt agtaaccaca tagtcgatgg acggctcgaa
    10261 ggaggccgga gtacgtccgc cagtgatgtc gttcatcagt tcgtcgaggg tgtaacccac
    10321 cgccagtttc gccgccactt tagcaatcgg gaaaccggtc gctttcgacg ccagcgccga
    10381 agaacgcgac acgcgcgggt tcatttcgat aacaatcagg cgaccgtttt tcgggttcac
    10441 cgcaaactgg acgttggaac caccggtttc aacgccgatt tcacgcagca ccgccatcga
    10501 ggcgttacgc atgatttgat attctttgtc ggtcagcgtt tgggctggcg cgacagtgat
    10561 ggaatcacca gtgtggatgc ccatcgcatc gaagttttcg atagagcaga cgatgatgca
    10621 gttgtcgttt ttatcacgca ccacttccat ctcgtactct ttccagccga tcagcgactc
    10681 atcaatcagc aactctttgg tcggagagag atccagaccg cgggcgcaaa tttcttcaaa
    10741 ctcttcgcgg ttataagcga taccgccgcc gctaccgccc atggtaaagg atgggcgaat
    10801 aatgcacggg aagcccacgt cagcggcaac cgccagcgct tcttccatcg tgtgtgcgat
    10861 accggaacgc gcggtttcca gaccaatttt cttcatcgct acgtcgaaac gacggcggtc
    10921 ttctgcttta tcaatcgcat cggcagtggc accaatcatg gtgacgccga actcttccaa
    10981 cacgccctga cgttccagct ccagcgcgca gttcagcgcc gtctgaccgc ccatggttgg
    11041 cagcaccgcg tccgggcgct ctttttcaat aatcttgcgt accacttccc agtgaatcgg
    11101 ctcgatgtag gtcgcatcgg ccatttccgg gtcggtcatg atggtcgccg ggttggagtt
    11161 caccagaatg acgcggtaac cctcttcgcg cagggcttta catgcttgcg cgccagagta
    11221 gtcaaactca cacgcctgac cgataacaat cgggcccgca cccagaatca ggatactttt
    11281 tatatctgta cgttttggca tggctctttt actcctgatt acttagcggt tttacggtac
    11341 tgctcaatta actcgataaa gtggtcgaac aacggcgcgg cgtcgtgtgg accagggctg
    11401 gcttcagggt gaccctggaa gctgaacgcc ggtttatcgg tgcgatgaat gccctgtaac
    11461 gtaccgtcga acagggattt atgcgtgaca cgcaggtttg caggtaatgt tgcttcatcc
    11521 accgcaaaac cgtggttctg ggcggtgatc atcaccacgt ttttctcaac atctttaacc
    11581 ggatggttgc cgccgtggtg accaaatttc attttgacag tcttcgcacc gctcgccagc
    11641 gccagcagct gatgaccgag gcagatgccg aataccggaa tagcggtttc gaggaatttc
    11701 tggatggcgg taatggcgta atcgcacggg gccgggtcgc caggaccgtt ggagaggaag
    11761 atgccgtctg gattcatttt cagcacatct tccgcagaag tttgcgccgg aacgatggtc
    11821 aggcgacagc ctctatccac cagcatccgc aggatgttgc gcttggcacc aaaatcataa
    11881 gccacgacgt ggaacggcag ctcgtcttct tttttcgctt ctggcaggcc gccggtcaac
    11941 gtccagctcc cttgtgtcca gctataggct tctgcggtgg tcacttcttt tgccagatcc
    12001 atgccgttca gacctgggaa cgcgcgggct ttttctaacg ccagcgccgc atccgggtta
    12061 tcgcctgcga taatgcagcc attctgtgcg cctttctcgc gcagtaaacg tgtcagctta
    12121 cgggtatcga tatcggcaat cgccacgatg ttatggcgct tcaggtaaga agataggtct
    12181 tcggtattac ggaagttgct ggcaatcagc ggcagatcgc gaatcaccag accttgtgca
    12241 tgtacctgag aagattcttc atcggcgtca ttagtgccga cattgccaat atggggataa
    12301 gtaagagtaa cgatttgacg agaataggaa ggatcagtga ggatttcttg ataaccggtc
    12361 attgaagtat tgaaaacgac ttccccaacc gccgaacctg ttgcccctat ggcccgaccg
    12421 tgaaactggg ttccgtcttc cagaaccaat agcgctgact taatcaaaac accctccaga
    12481 gaatattcac tcactttatt tgcatattaa ttcacaatga tgacgtaaat caatgcaaat
    12541 ctgcttaccg acgaatttct ggcaaacggc ggcattctgg agatatgggc gctaaaagtc
    12601 aacttaatgg cgggattttt tatattattt tgtggctcat gcatgtttta caggcattaa
    12661 gcgacaaaaa gatcatctaa cctgttctgg aaaacgcttg cgcagcaaga agattgcatg
    12721 aagtaaaaac gagtagaaaa aagtggacca aatggtcaaa atttacatta aaacaacaaa
    12781 aatagaagac aaaacatgtg atttatagcg tttgcataaa atttaaaagc ataacaccaa
    12841 aaataaaagg gctaaaaatt aaagcccttt atattcaata aattaaatgc gttacttttg
    12901 catcaccata aacactttgt ggttacaaat tatggagatc aagcacatct cgcatatcaa
    12961 aaagaccact ttccttacca ctcaaccaca aagccgatct taccgcaccg ttagcaaatg
    13021 tcatgcggct ggacgcctta tgggtgatct ccagacgctc gccaatatcg gcaaacatcg
    13081 cggtatgttc gccaacgatg tcacctgcac gcacggtggc aaaaccaatg ctaccaggca
    13141 cacgttcacc ggtatggcct tcacgactgt agaccgcgca atctttcaga tctttatcaa
    13201 gggcgtgggc gatcgcctct cccattgcca gtgcggtgcc tgacggcgca tcaactttat
    13261 gtctatgatg tgcttcaata atttcgatat cggtgtagtc acccatcact ttggctgctt
    13321 tctccagcag cttaagcatg acgttaacgc caacgctaaa attggcggca aatacaatcg
    13381 caatatcggc ggcggcgtca cgaatcgcct gtttaccggc ttcgtcaaac cccgtagtgc
    13441 cgatcaccat ccctttgcca tgctggcgac aaaaagcgag atggttcagc gtaccttccg
    13501 gacgggtaaa atcgataaac acatcaaaat catcttttat cgcatcaagg ctgctttgca
    13561 cggtaacgcc tgttttccct gcaccagcca gctcaccggc gtcgctgccc agtaaagaag
    13621 atccttcgcg ctccagcgcc gcgcccagct gcacgccttc taatgccagc gccgcctgaa
    13681 tcaactggcg gcccatacgc cccccggctc ccgcgatggc aacgcggatg tttgcatcat
    13741 gcatagctat tctcttttgt taatttgcat agaccgtttt cagagtaacc agcgcatcgc
    13801 tacgccgcca gccaaaaacg ctgataatta gaaattaatg ataaacaggg agggatatca
    13861 gtgaaaggca tgagtcgatg aatgactgca tgccaataac atgtgacagg ttacggcgcc
    13921 agagccagca cctcagccac ccactgctgg aagcctttta cattcagatc caatgccacc
    13981 tgtacattgg ctggtttgcc caggcaaccg tcgatatcaa ccaccgtcgt gcctgaggta
    14041 aattcgcctt gagcttctac cgcgacaaaa cacggcttga gggtgaataa gtccgggcgc
    14101 accagccagg cgatggcgca gagatcgtgc attcgcagac cgctttgcat actgccgcta
    14161 cggtagtggc taaacagggc gtggagcatt tttccggtgc gattcaactc cggtaaagta
    14221 gcgagatagt caggggttaa tattgcctga ttggtgacat ccagaccgca catgacaatt
    14281 tcgatgccgc tacggaagac acaggcagcc gcttctggat cggcagcgat attaaactcg
    14341 gcgtttggtg tgcagttgcc gcgaacggca gaaccgccca tgatcaccag acgacgaata
    14401 tacggtttac attccgggca ttgtgaaagt aacagcgcaa tattggttaa cgggccgatg
    14461 gccaccaggg taacgggctc tggtgcacgc atcagggcat cccgaatcgc cagaaacgcc
    14521 ggtatcccga gtggctttcg gttgtgctca acaaagtcgt agccagccat tcccgattcg
    14581 ccgtgcacag atgctgcatc acgcggtgcg cgtaccagcg gcacagcggc tccctgggcg
    14641 agcggaatct ccgcattcca gaaatgcagc agttgcaggg cgttgcgggt ggttttctca
    14701 accgagacat tacccgcgac ggtggtcatc agttgcaggt cgagttcggg ggcaaaaatc
    14761 gcggcggcaa tggcgacggc atcgtcaatg ccggggtcag tatcgaggaa gataggtaaa
    14821 cgcatgtttt ctccataaaa aatgccggta acaagaccgg cattttcgca taacttaggc
    14881 tgctaatgac ttaatcgact tcacgaatat cgacacgcag ctctttcggc acttcgaaaa
    14941 caatgttttc ttcacggcct tccagcggaa tggcttcacc accgcccagc tgctgcaaac
    15001 gtgccaccac attctgcacc agaatatccg gagccgatgc gcccgcagtc acgccgacgc
    15061 attttacctc tttaacccac tcttcctgga tatctttcgc atcgtcaatc aaaaacgcgc
    15121 gtttgcccat gcgctgggcc agttccgcca gacggttgga gttggaggag tttttcgaac
    15181 cgaccaccag caccacttcc gcctgctctg ccagggcgcg taccgcttcc tgtcggttag
    15241 tcgtggcgta gcagatgtca tctttgcgcg gaccgacaat tttcgggaag cgtttacgca
    15301 gcgcgtcgat cacatcagac gtgtcatcca ccgacagcgt ggtttgggtc ataaaggaga
    15361 gtttctcttc gtttttgacc gtcagtttcc acacatcgtc tggcgattcg accagataca
    15421 ttcccccttc cgggttgctg tactgaccca tcgtcccttc cacttccggg tgcccggcgt
    15481 ggccgatgag aatagattct tcgccacgac ggctggcgcg ggcaacttcc atatgcactt
    15541 tggtcaccag cggacaggtg gcatcaaaca ccgtcaaatc gcggcttttc gcttcgttac
    15601 gaaccgcctg agaaacaccg tgtgcggaga aaatcaggat cgcgccgtcc ggcacttcgc
    15661 taatctgctc aataaagata gccccacgct cgcgcaagct atcgaccacg tagcggttat
    15721 gcaccacttc gtgacggaca tatatcggtg cgccgtaaat ggccagcgcg ttttcaacaa
    15781 tgctgatagc gcggtctacc ccggcacaaa aaccacgcgg gttggccaac aggatctgca
    15841 tgttacgcct ccagtgccgg atcgatttcc agcacttcaa tatcaaaatg aacggtctgc
    15901 ccggccagcg gatggttgaa atcaacggta atggagtcgc cgttaatttc gcggatcacg
    15961 ccaggcatct cactgccatc cattgcggta aaaagcataa ttgcgccaat ttctggctcg
    16021 cctgcatcca taaattcacg gcgggagaag tactgaatca ggtccggtga cggcacgcca
    16081 aacgccgcat cgggctccag cgagaaggtg gttttatcgc ccactttcag ccccagcagg
    16141 tgttgctcca gcccttcaga aagagaagca tcgcccaggc ggaacagcgc cggtttaccg
    16201 ttgttgcggg tagactcggc ggtggtgcca tcgtcgagtt ttagcgtgaa gtgcaccagg
    16261 acggcgctat tgctctgtac agattcagac atgcaggttt gctcttatat tttgaggatt
    16321 tttgccggat gcggcctacg atttcgcagc aatctgtagg ccttatccgg cagggtttat
    16381 tattgttttt tcgctttaga aggcaaaaaa ccttccagca caatcagtgc cgcaccgaca
    16441 cagatggcag tatcggcaag gttgaaggtg gcgaagtgcc agtcgccgac gtagaagtcg
    16501 atcatatcga caacgaagcc gtgccacagg cggtcgaaca ggttgcccag cgcgccgcca
    16561 ataatcagcg cgtaagcgat attgtttagc ttctgcgtgg ccttcgagcg atacatcatc
    16621 actgccagga tcacgctaat accaatcgca ataccggcaa agaaccaacg ctgccagccg
    16681 ccgctatcgg caaggaaact aaacgccgcg ccatagttac gcgcataatg cagattaagc
    16741 gacgggaaca gcgggaccgt atcccccaga gcaaagttct ggaggatcag gtatttgctg
    16801 cccagatcga taatcagcac gactaccaca agccacagcc agcgtagccc tgttgaacag
    16861 atcgattgac tcatcaggca aacttacgtt tttcaccgtc accggcgacg ttgctgacac
    16921 agcggccgca gatttctgcg tgttccgcca ccttgccgac atcctgggtg tagtgccagc
    16981 agcgtgggca cttctcacct tcggctttac tcaacgcgac tttcagtcct ttgagtactt
    17041 cgctctgctg agcatcagca ggtgcgtcgt tatagtctgc aacggtagcg ccggaggtca
    17101 acaggacaaa tcgtaattca tcgcccagcg cggtcagttt cgccgccagt tccggttctg
    17161 catacaaggt tactgccgct tccagcgagc cacccacttt cttgtcggca cgcgcttgct
    17221 caatgacttt gttcacttcg ccacgcactt tcaacagctc gtcccagaac gcatcgttca
    17281 ttgcttcact gtctgccaga ccaaacaggc cttcgtacca ctcaccggtg aagacgtatt
    17341 tttcacgttc gcccggcaga tagccccaca cttcatcagc ggtgaaggag aggattggtg
    17401 ccatccagcg caccagcgct tcagcgatgt gatacagcgc agtctggcag ctacgacgcg
    17461 ccacgctatc cgctttggcg gtgtactgac ggtctttgat gatgtcgagg tagaaggaac
    17521 ccatctcaac ggagcagaag cgcatcagac gctgtaccac ttcgtggaaa tcgtatgctt
    17581 cgtacgcctt gaggatgtct tcctgtgccg ctttcgcaca acctacggcc cagcgatcca
    17641 gtaccaccat ctcttccggt ttcaccatat cttttgccgg atcaaaaccg ttcaggtttg
    17701 ccagcaggaa gcgcgcggtg ttacggatac gacgatagct gtcggctgca cgtttcagga
    17761 tctcgtcaga aacggccatt tcaccggtgt agtcagttga tgccacccac agacgcagaa
    17821 tatccgcgcc cagtttgttc atcacatcct gcggcgaaac ggtgttgccg atagatttag
    17881 acatcttgcg gccctgacca tccacggtaa agccgtgggt cagtacctga cgatacggcg
    17941 ctttgccctt catcgcggtg gagatcatca aagaagacat gaaccaacca cggtgctggt
    18001 ctgaaccttc cagatacatg tccgctgcgt gaccggcaaa ttccggacgc acatcaacaa
    18061 cagaagagtg ggtagatccg gagtcaaacc atacatccag cgtatccggc actttcacat
    18121 actggtcagc ttcgtcgccg aggatctctt tcgcatcgag atcccaccac gcctggatgc
    18181 cgtcaacttc aacgcgttta gccacttctt ccatcagttc gagggtacgc ggatgcagct
    18241 cttccgtgtc tttgtgcacg aacagtgaca tcggtacgcc ccaggtgcgc tgacgggaga
    18301 tacaccagtc aggacggtta gcaaccatcg actcgatacg cgcctggccc cagtccggga
    18361 tccactgcac gcctttgatc tctttcagtg actgcgcacg cagacctttc tgatccatgc
    18421 tgacgaacca ctgcggcgtc gcgcggaaga tgatcggcgt tttgtgacgc cagcagcacg
    18481 gatagctgtg ctgcattttc tcaacgtgca gcagcgcgcc tttttcctgc agcagcgcaa
    18541 cgacgatgtc gttcgctttg aagacgttca cgccatccag cgtcggataa gtgcccggca
    18601 gataagtgcc gtccgggcca accgggttag cggtttccag gccgtatttc tgaccgatca
    18661 catagtcgtc cgggccgtgg ccaggcgcgg tgtgaacggc accggtaccg gcatccaggg
    18721 taacatgatc gccgaggatt gccgggacgt cgaagcccat aaacggatgg gcaaagcgca
    18781 gcagctcaag ctccgcacct tttaccgtgc cgagaatggt gtaatcggtc acgccgatac
    18841 gctgcattac gctttcaacc agatctttcg ccagaatcac ggcctgaccg tcgatctgca
    18901 ccaacgcata gtcgaaatca ggtgcaatag agattgcgcg gttcgcaggc agagtccacg
    18961 gcgtagtggt ccagatcacc agcgagattg ggccgttaac gttgcttacg ccaaatttcg
    19021 ctttcagcgc atcctgatcg accgcctgga aagcaacgtc aatggacgga gaagttttgt
    19081 cgtaatactc aacttccgct tctgccagtg cagaacggca gtctacgcac cagtgcaccg
    19141 gcttcgcgcc tttgtgcagg tgaccattgc cgatgatttt gcccagcgcg cggatgatat
    19201 tggcttcagt tttgaagtcc atggtcaggt acgggtgcga ccagtcgccc agcacgccca
    19261 gacggataaa gtctttgcgt tgaccgtcaa cctgggtcgc cgcgtattca cggcacttgg
    19321 cgcggaactc ggcggcggtg aatttctcac ccggcttacc gtattcttgc tctactttca
    19381 gctcgatcgg cagaccgtgg cagtcccagc caggcacata cggcgagtca taaccggaaa
    19441 gccctttgga cttcacgata atgtctttca gaatcttgtt aaccgagtga ccaatatgaa
    19501 tgctgccatt cgcataagga gggccatcat gcagaatgaa ggtttttttg ccttttttag
    19561 ccgcacggat gatgccgtac agatcatcat cagtccaacg cgccagcatt cccggttcgc
    19621 gcttggcgag atcgccacgc atcgggaacc ctgtttccgg caaattcagg gttgatttat
    19681 agtcactcat cagattctcg gttccgtgtt tcggtttgat tacataacag gcttaagccg
    19741 gttttgttag cccaaaaaat tcgcgggcgg ttaattcatc acgcgcaatc tgcgctttca
    19801 gttcgtccag cgacgcaaat cgctgctcat tgcgtatttt tttacgcagc actacttgta
    19861 tatggcgacc gtaaaggtcc attgcaacat ctaacaaatg cacttccagc tgctggcgaa
    19921 taccggcaac cgttgggcgt gttccgatgt ttgccacgcc gggtaacggc ttttcaccga
    19981 ggcccagcac ttctaccgca taaacccctt tcaccgggga aacctgacgg cgtagcggta
    20041 cattcgccgt cgggaaacct atagtgcgcc ctaattcatc accgtggact acacgcccgg
    20101 agatggcaaa cgggtgcccc agtaaacttt ctgccagagc cagattgtca tcagccagcg
    20161 cctgacgcac ggcggtgctg ctgatacgca caccaccttc gcaaaaagtt tgcgtactgg
    20221 tgatatcgaa gccgtattcc atgccagctt tctgtaataa caagaaatcg ccttcacgac
    20281 cagcgccaaa gcggaaatca tcacctacgg caagaaattt tacgcgcaaa tgcttcacca
    20341 aaagatcgct gatgaaattt tgcgcggtta acgccgcaaa acgcctgtcg aaacgcacgc
    20401 acagcacgta atcaacgcca cactctgcaa ggtaacgcag tttttcccgc agccgggtca
    20461 gccgtgccgg ggctttatcg gtagcaaaca gttccagtgg ttgaggttca aaaagcatca
    20521 ccatcaccgg taagttgcgc ttgcgccctt cttcctgcaa gccctgtaac agcgcgcgat
    20581 gaccgcgatg cacgccgtcg aaattaccaa tagtcagcac acacccttct tgcggggcct
    20641 ggctgagatt atgtatgccg cgtatcagct tcatgtctgg ctcaaaacag tgaaaatcgt
    20701 ccgagtatac cttgtacagc ggtcaaggtt aaccggcgat tgagtaccga aatcagaaag
    20761 aagtggcttt tcattgccat ggcgcaaatc acgggaagaa actgaccgcc tgctgcaatt
    20821 tttatcgcgg aaaagctgta ttcacacccc gcaagctggt agaatcctgc gccatcacta
    20881 cgtaacgagt gccggcacat taacggcgct tatttgcaca aatccattga caaaagaagg
    20941 ctaaaagggc atattcctcg gcctttgaat tgtccatata gaacacattt gggagttgga
    21001 ccttggctaa tatcaaatca gctaagaagc gcgccattca gtctgaaaag gctcgtaagc
    21061 acaacgcaag ccgtcgctct atgatgcgta ctttcatcaa gaaagtatac gcagctatcg
    21121 aagctggcga caaagctgct gcacagaaag catttaacga aatgcaaccg atcgtggacc
    21181 gtcaggctgc taaaggtctg atccacaaaa acaaagctgc acgtcataag gctaacctga
    21241 ctgcacagat caacaaactg gcttaatcgc caatttgctg aagctttgtg aaaaagcccg
    21301 cgcaagcggg tttttttatg cctgctgctt ttgtgatgcg tgagagaaca cactgccggt
    21361 tgccagatag tcaaaagttg caggttcttg cagacatgcc gttgcactat ctgaactctt
    21421 cgtggctaaa gaatcgtaga accatcggct gatacattct gaacaagaat atctactcaa
    21481 tctccagact accctacaaa gaatgtggat attgtgatac acattgaggt attaaactta
    21541 agaccactct atttttgaat taaattccta tatact
//
LOCUS       DAZKFU020000063        21531 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZKFU020000063 DAZKFU020000000
VERSION     DAZKFU020000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.063"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..655)
                     /gene="parM"
                     /locus_tag="ACHOQQ_RS09280"
                     /old_locus_tag="pgaptmp_001856"
     CDS             complement(<1..655)
                     /gene="parM"
                     /locus_tag="ACHOQQ_RS09280"
                     /old_locus_tag="pgaptmp_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HGU4470120.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     "
     gene            1094..1996
                     /locus_tag="ACHOQQ_RS09285"
                     /old_locus_tag="pgaptmp_001857"
     CDS             1094..1996
                     /locus_tag="ACHOQQ_RS09285"
                     /old_locus_tag="pgaptmp_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU4470121.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            1993..2303
                     /locus_tag="ACHOQQ_RS09290"
                     /old_locus_tag="pgaptmp_001858"
                     /pseudo
     CDS             1993..2303
                     /locus_tag="ACHOQQ_RS09290"
                     /old_locus_tag="pgaptmp_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2380..2451
                     /locus_tag="ACHOQQ_RS09295"
                     /old_locus_tag="pgaptmp_001859"
                     /pseudo
     CDS             2380..2451
                     /locus_tag="ACHOQQ_RS09295"
                     /old_locus_tag="pgaptmp_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
     gene            complement(2533..4233)
                     /gene="ltrA"
                     /locus_tag="ACHOQQ_RS09300"
                     /old_locus_tag="pgaptmp_001860"
     CDS             complement(2533..4233)
                     /gene="ltrA"
                     /locus_tag="ACHOQQ_RS09300"
                     /old_locus_tag="pgaptmp_001860"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGU4470122.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWARYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKLLPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNIGNLAMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            4860..5471
                     /locus_tag="ACHOQQ_RS09305"
                     /old_locus_tag="pgaptmp_001861"
                     /pseudo
     CDS             4860..5471
                     /locus_tag="ACHOQQ_RS09305"
                     /old_locus_tag="pgaptmp_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            5472..5693
                     /locus_tag="ACHOQQ_RS09310"
                     /old_locus_tag="pgaptmp_001862"
     CDS             5472..5693
                     /locus_tag="ACHOQQ_RS09310"
                     /old_locus_tag="pgaptmp_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470123.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            5623..6141
                     /locus_tag="ACHOQQ_RS09315"
                     /old_locus_tag="pgaptmp_001863"
     CDS             5623..6141
                     /locus_tag="ACHOQQ_RS09315"
                     /old_locus_tag="pgaptmp_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU4470124.1"
                     /translation="MAPIACSWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAKY
                     PGDTPLMLVIWSPEEIQALSDGMDIALTDHEIRTVLARLEDIPEDQRTESGISSGVAM
                     EIINNVSENRQVTVPAELLASLIQTAEQALWKREWAARDHGLAVPECVTRRQAVVNQV
                     RILLKNNTHEND"
     gene            6186..6956
                     /locus_tag="ACHOQQ_RS09320"
                     /old_locus_tag="pgaptmp_001864"
     CDS             6186..6956
                     /locus_tag="ACHOQQ_RS09320"
                     /old_locus_tag="pgaptmp_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470125.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLGAVRDAVCSVSYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDMLR
                     IPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            7371..7796
                     /locus_tag="ACHOQQ_RS09325"
                     /old_locus_tag="pgaptmp_001865"
     CDS             7371..7796
                     /locus_tag="ACHOQQ_RS09325"
                     /old_locus_tag="pgaptmp_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4470126.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            7843..8262
                     /locus_tag="ACHOQQ_RS09330"
                     /old_locus_tag="pgaptmp_001866"
     CDS             7843..8262
                     /locus_tag="ACHOQQ_RS09330"
                     /old_locus_tag="pgaptmp_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU4470127.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            8262..8453
                     /locus_tag="ACHOQQ_RS09335"
                     /old_locus_tag="pgaptmp_001867"
     CDS             8262..8453
                     /locus_tag="ACHOQQ_RS09335"
                     /old_locus_tag="pgaptmp_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470128.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            9492..9722
                     /locus_tag="ACHOQQ_RS09340"
                     /old_locus_tag="pgaptmp_001868"
     CDS             9492..9722
                     /locus_tag="ACHOQQ_RS09340"
                     /old_locus_tag="pgaptmp_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470129.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            9774..11135
                     /locus_tag="ACHOQQ_RS09345"
                     /old_locus_tag="pgaptmp_001869"
     CDS             9774..11135
                     /locus_tag="ACHOQQ_RS09345"
                     /old_locus_tag="pgaptmp_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HGU4470130.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPEQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDRYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQRQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            11182..11745
                     /locus_tag="ACHOQQ_RS09350"
                     /old_locus_tag="pgaptmp_001870"
     CDS             11182..11745
                     /locus_tag="ACHOQQ_RS09350"
                     /old_locus_tag="pgaptmp_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGU4470131.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            11745..11987
                     /locus_tag="ACHOQQ_RS09355"
                     /old_locus_tag="pgaptmp_001871"
     CDS             11745..11987
                     /locus_tag="ACHOQQ_RS09355"
                     /old_locus_tag="pgaptmp_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470132.1"
                     /translation="MTQGWREDKCPSVRAGKKPRAGGAERGRAHAPPLSLRGRSDGGF
                     CLFRPVICLLLMMLLFLLLLLLLLLLLLLLFFFLPL"
     gene            11897..12157
                     /locus_tag="ACHOQQ_RS09360"
                     /old_locus_tag="pgaptmp_001872"
     CDS             11897..12157
                     /locus_tag="ACHOQQ_RS09360"
                     /old_locus_tag="pgaptmp_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470133.1"
                     /translation="MSVTDDVVVPATATATATATATALLFPAALILTTAVLSCLPGAP
                     SAPGCLAASGRQARLRQAAAGAPASLRRTRWRGFGLSLRSDA"
     gene            12628..13149
                     /locus_tag="ACHOQQ_RS09365"
                     /old_locus_tag="pgaptmp_001873"
     CDS             12628..13149
                     /locus_tag="ACHOQQ_RS09365"
                     /old_locus_tag="pgaptmp_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4470134.1"
                     /translation="MAVRGINKVILVGRLGKDPDVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPEPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            13207..13440
                     /locus_tag="ACHOQQ_RS09370"
                     /old_locus_tag="pgaptmp_001874"
     CDS             13207..13440
                     /locus_tag="ACHOQQ_RS09370"
                     /old_locus_tag="pgaptmp_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HGU4470135.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNRYIRDFGILK"
     gene            13501..15459
                     /locus_tag="ACHOQQ_RS09375"
                     /old_locus_tag="pgaptmp_001875"
     CDS             13501..15459
                     /locus_tag="ACHOQQ_RS09375"
                     /old_locus_tag="pgaptmp_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGU4470136.1"
                     /translation="MSVTKSEPKSTRKASRKLAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVCVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMESVGFCREDAGTYRCLPEPESVLTEAEE
                     ERLNELMARYDALENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDPAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPVGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMGSPRPQTDATERADNLADAA"
     gene            15514..15948
                     /gene="psiB"
                     /locus_tag="ACHOQQ_RS09380"
                     /old_locus_tag="pgaptmp_001876"
     CDS             15514..15948
                     /gene="psiB"
                     /locus_tag="ACHOQQ_RS09380"
                     /old_locus_tag="pgaptmp_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGU4470137.1"
                     /translation="MKTELTLDVLQTMSAQEYEDIRAAGSGERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFVPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            15945..16664
                     /locus_tag="ACHOQQ_RS09385"
                     /old_locus_tag="pgaptmp_001877"
     CDS             15945..16664
                     /locus_tag="ACHOQQ_RS09385"
                     /old_locus_tag="pgaptmp_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HGU4470138.1"
                     /translation="MSVRSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRWISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERRRHKSALVMQKYSRQQAREAEQKTRAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            16661..16975
                     /locus_tag="ACHOQQ_RS09390"
                     /old_locus_tag="pgaptmp_001878"
     CDS             16661..16975
                     /locus_tag="ACHOQQ_RS09390"
                     /old_locus_tag="pgaptmp_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HGU4470139.1"
                     /translation="MKWRYSLRWKLPYPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            17125..17277
                     /locus_tag="ACHOQQ_RS09395"
                     /old_locus_tag="pgaptmp_001879"
                     /pseudo
     CDS             17125..17277
                     /locus_tag="ACHOQQ_RS09395"
                     /old_locus_tag="pgaptmp_001879"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            17222..17344
                     /locus_tag="ACHOQQ_RS09400"
                     /old_locus_tag="pgaptmp_001880"
     CDS             17222..17344
                     /locus_tag="ACHOQQ_RS09400"
                     /old_locus_tag="pgaptmp_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4470140.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
     gene            complement(17798..17971)
                     /locus_tag="ACHOQQ_RS09405"
                     /old_locus_tag="pgaptmp_001881"
                     /pseudo
     CDS             complement(17798..17971)
                     /locus_tag="ACHOQQ_RS09405"
                     /old_locus_tag="pgaptmp_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18270..18572
                     /locus_tag="ACHOQQ_RS09410"
                     /old_locus_tag="pgaptmp_001882"
     CDS             18270..18572
                     /locus_tag="ACHOQQ_RS09410"
                     /old_locus_tag="pgaptmp_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470141.1"
                     /translation="MSAFSFNTAYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLLKQLWQINDELGELLDSVENHTPVIRKKASDLSALMMLFSQQAGQPMTMLLIC"
     gene            18728..19015
                     /locus_tag="ACHOQQ_RS09415"
                     /old_locus_tag="pgaptmp_001883"
     CDS             18728..19015
                     /locus_tag="ACHOQQ_RS09415"
                     /old_locus_tag="pgaptmp_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470142.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRTGLDIRAIC"
     gene            19134..19955
                     /locus_tag="ACHOQQ_RS09420"
                     /old_locus_tag="pgaptmp_001884"
     CDS             19134..19955
                     /locus_tag="ACHOQQ_RS09420"
                     /old_locus_tag="pgaptmp_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4470143.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLREEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVPGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRWEDKKNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRGITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(20252..20842)
                     /locus_tag="ACHOQQ_RS09425"
                     /old_locus_tag="pgaptmp_001885"
     CDS             complement(20252..20842)
                     /locus_tag="ACHOQQ_RS09425"
                     /old_locus_tag="pgaptmp_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGU4470144.1"
                     /translation="MVRTTNLLSLFVEWVKLFPDDVTRGEKMRKWLLTICLMFINVTC
                     KAGDCFDLAGRDYKIDPDLLRAISWKESRNRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTADPCLNIYTGAYYLAIAFKKWGVFWEAVGAYNAGFRKTERQNQRRLA
                     YASDVYRIYSGIKNSKGIQLRASEKPLSKMNSSQKN"
     gene            21175..>21531
                     /gene="traM"
                     /locus_tag="ACHOQQ_RS09430"
                     /old_locus_tag="pgaptmp_001886"
     CDS             21175..>21531
                     /gene="traM"
                     /locus_tag="ACHOQQ_RS09430"
                     /old_locus_tag="pgaptmp_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HGU4470145.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQIEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMER"
BASE COUNT         4776 a         5580 c         6137 g         5038 t
ORIGIN      
        1 gtgtggaggc ttttgttagt gcattcatga cggtatgagt tatgcgggat gtcccgattt
       61 ctgaatagcc acggatttca gaaatgcctt caaatgcacc gagaattaaa ccacaatcca
      121 gagtggttcc tccaagatcg ataatcaggc tgcgttcaag tttgtttact ttatcttttt
      181 ttagtgattc aaatgcagct ggcaaagatt caggcattac attaactttt ttaattttaa
      241 atgtttcccc tttattcaga gatatctcac ggagaacatt tgcctttttt ctttctattc
      301 tttcttcatt tggttgattg tcattatcaa agaattcggt tacaggtaat gtcactgtca
      361 gttctacatc ctgtggctga aggccggatg tcagcagtgc atgatgtatg gcaagcagat
      421 tggtggtgct gtattgataa ctgacatgtg ttgtacctat gacagcagtg ctgcccaaat
      481 cataggtgta tttttttcca tccacgacgt aattgtaaac tttgccggca ttaaacagtc
      541 ccgggttcca gccttcttta aaactgtttc ctgacagact ggttcttacc tcgccatctt
      601 caagccaggc tagttttacg ttagttgaac catcatcgca tgatactttt agcattcttt
      661 ttgcctcgtt ttgagtttgt gtttgtctca aattgagcat cttttatgtc gatgcatctt
      721 tttgagtttt tgttatttct tattgtgtct cattttgggt taatgtcaag tagcgtggct
      781 cttttagagt ctccttgtgt tcttgctgtg actcaatttg agttgttatt ggtttctcat
      841 tttgcgtcac aaactgagac gaaaactact taaccaggcc gtgccggcac gttaaccggg
      901 ctgcatccga tgcaagtgtg tcgctgtcgc cggcctcctc acccggtcac gtttcgtcgt
      961 tcctcctcca cgcgctgcgg cttcggggcc gcacctgcat tcgtatgcgg tcgcccggtt
     1021 acaggtgcgg cacggcctga tggaggccgc atgtgagagg agaattccca tgccaaactg
     1081 gtgttcgaat cgtatgtatt tttctggtga accggcacag atcgctgaga ttaaacgact
     1141 ggccagcggt gcagtcacac cgctttatcg ccgcgccaca aatgaaggta ttcagctgtt
     1201 tctggccgga agtgccggac ttctgcagac cactgaagat gtgtggtttg aaccgtgccc
     1261 cggactgacg gctgccggac gtggcgttgt atcgccggag aatatcgcgt tcacccgctg
     1321 gctgacacac ctgcagaacg gtgtactgct ggatgagcaa aactgcctga tgctgcatga
     1381 actctggctg cagagcggta ctggccagcg tcgctgggaa ggattaccgg acgatgtcag
     1441 ggataccatc accgcgcttt tcaccgcaaa aagaggtgac tggtgtggct tctggagtaa
     1501 cgaggatgta tcggtgtggt ggaaccgtct gtgtgacaac gtactgccgg aaaaaaccat
     1561 gccgtttgac ctgctgacgg ttctgcccac ccgccttgat gttgaagtga atggctttaa
     1621 cggtggtgtt ctgaacggtg ttccttctgc atatcactgg tataccgaac agtatggcgt
     1681 gaagtggcct tgtgggtatg acctgaatat cagcagtcag ggagacaact tcattcaggt
     1741 tgatttcgac acgccgtggt gtcagccgga aagcgacgtt attgcagaat taagccgccg
     1801 tttcagctgc acgctggagc actggtatgc cgaacagggc tgtgatttct gtggctggca
     1861 gttgtatgag cgcggagagc tcgttgatgt gctgtggggg gagctggaat ggtcttcccc
     1921 gacagatgac gatgagctgc cggaagtcac cggacctgcg tggatagtcg acaatgtggc
     1981 gcattatggc ggatgaagta tgacggagac gggcgggcaa ccgcccgttt cttttccgac
     2041 aaaggatgtc gccgtgctcc tctttttact gcgccggctg acgcggcgcg gcaggaacgc
     2101 tgcctgtggt cagtgtcccg cgtccggcgg gcacgggacg ggcgctttta ccgctcccgg
     2161 ctggcgtcgg tgacagtgta cgccagcccg tcgccctttt ctgatgaacg cccttcaagc
     2221 cgctttcgcg gcataacctt gccgtcagaa agacgacggc aaggtattcc acggtcggcc
     2281 tgacccgtta ccggacgcgg tgaacagccc acaggcagcg gggaacgggc gccacagggg
     2341 cgccctcccg gtgctctctg taagagaagg agtgttgtta tgtcccgttt tatcctgggt
     2401 gactgcgtgc gtgttatggc cacgtttccg ggcaatgctg tcgatttcat gtcgggtagc
     2461 agctgcacat tactgtgcag ccgcccccta agaaccgtac gtgccagttt cccggcatac
     2521 ggctcaagcc tttcaataac tccacattag agccggcttc ataacagata ttccctgatg
     2581 ttttgcagtg tgatgacatg ccggatgtaa catggccaga ttacctatgt tatccagtcc
     2641 cccttcggat ttcggcacaa tgtgtcgaag ttgccagcct gttgtggcgt tcactggcag
     2701 gtggcagacg gggcactgac ctccctgtcg gagccacaga tttcttaggc gagagtaacc
     2761 ctgtatggat tccagcatcg tagcttttag ccgtgcttca aagtacgatt cgtagctggg
     2821 atcaaaaggg ttagcagtgc tttttatttg tctgtgtctt ttgactggca tatccgagat
     2881 ttcatatagc gccagtaact cacgtttgcc tgatggcagg agcttccctg tttctgtgcc
     2941 gaatacccag tgtcggaagt taactgaacg aaagtatttc tttcttatcc agcctgtacc
     3001 tttctttggg tgtcgtcgac atgcccagcg ccatagtgct ttccatattt catgactgac
     3061 tttctgaaag atcttttttg aaacaacatg acgatagtat ctggcccagc ctttgaggac
     3121 tgggttgaga tgccatatca gtgtgctctg gctgactgtc ggattgtctt ttatcgtctg
     3181 gcggaccctt ttcaggaatt cctttacctt ttggggtgaa ggtttaatca acagtttccc
     3241 gttatatttg cggatgttca tacccagaaa gtcgaatcca tcactgatgt gtgtgattcg
     3301 tgtcttttct ggtgagaggg taagccctct ttctgccaga aagttttcta acaagggaag
     3361 aaccttttct gacagcgtgt cgtggcttat ccccgtgatc acaaaatcgt ccgcataacg
     3421 gattatgttg atcttttctg gattcagtcc gcctcgatag aaggttttct tcaacagagg
     3481 ctgcagacca tccagaacca tgtttgccag cgttggagat attattccgc cctgtggagt
     3541 tcctgcactg gttgggtata atttccccaa atcaatatat ccggctttca gccatcgttt
     3601 caggatcgcc gtatccgttg ggatattggc gattagccat tcatgtgaaa tctcatcaaa
     3661 acaactgcga atgtcggctt ccagaatcca ttgtgcccga tcagccctgt gcaacgttgt
     3721 gaaacattgc tcaatggcat cggctgttga gcgacagggc ctgaacccaa acgaatttgg
     3781 gtcagccgtt gtttctgcaa caggttccag cgccatcaga tagagtgcct gcatggctct
     3841 gtctttcatt gtcgggatcc ctaatggtcg ctttttgccg ttagattttg ggatatatat
     3901 acgccgtagc ggaagaggtt tgtaacccct tcttttcagc gtgtatatag ccttcatctt
     3961 gctgccgggt tttgaccatg ttttgccatc aacacccggc gttctcttcc ctctgttatc
     4021 tgtcactcgt ttaactgcca ttgctctggc gctgaacgag tgggtgagta gccattgcaa
     4081 ggcttttacc ttgcccgctt tctcctcctg tattgccttc acgatacgcg cctgcagcct
     4141 tctgacctcg cgacggcact tggcccagtt gatattgtgc cagtgagtca cgatgtcggt
     4201 gggtgcaccc gcgtttatgt ttttcgcagt catctgctta ccctccttca aaggtattca
     4261 ggttatctcg tgttgaaaga ccatgcggaa gtctgcccgc tttcgcgtgg aatgatgttg
     4321 ctgaaacagc tcaactcctg tccatcccat tacaggatgg cattcgcttt ctccgcactc
     4381 ctgtacccgc atcactgaca gctttcctcg cggttagcct gccctgaggg cagtgatacg
     4441 ggcttaccgt gttccgcaca gtttgcaggg atgggatggg ttaggttctg cctatccacc
     4501 ggtagctttg atgtccgtgc ctccccagtc aaaagaggaa ggtccagcta cgttcccatt
     4561 ttgggtcggg cctgtcagtg cctttggccc gttcagggtt gacgatgttt atcagcagtt
     4621 cacatgtgtt aaccctgcca tccagccttg cgccccatcc gccctgacac tggcagacat
     4681 gcttcctcct cgcggattcc acatggtgtg aaaaccgggc tacgttgtcc cgaaggcttc
     4741 ataccgaaca gttacccgct ctgcatgctt cggtaggcta caggcgacgg aacgcctggt
     4801 tttactactt cctttacatg aagttaaaac aacaaactgt gcgactttca cgtcgcacac
     4861 tgactgaccc gccgtacctc gtcggttttc gtgaccgttt cgggcgcacc atcgctggcg
     4921 ataaaaccga tgaatggctg caaccggcct gtaatgaaat gtaccgcgta ctcaaaaaag
     4981 acgcgctgat gatcagcttc tacggctggc accgtattga ccgctttatg gcggcctgga
     5041 aacgcgccgg attcagcgtg gcagggcacc tcgtgttcac caaaaactac acatccaaat
     5101 ctgcgtatgt cgcgtaccgc cacgaatgcg cgtacatcct ggcaaagggc agtccggccc
     5161 ggccccgtaa cccgctgccg gatgtgctgg gctggaaata ttcgggcaac cgccatcacc
     5221 cgacggaaaa gcccgttacc agcctgcaac cactgattga gagcttcaca catccgaacg
     5281 cgattgtgct ggacccgttt gcaggcagcg gctcaacctg cgtcgctgcc ctccagtccg
     5341 gacgccggta tatcggtatc gagctgcttg aacagtatca ccgtgccgga cagcaacgac
     5401 tggccgccgt gcaacgggcc atgcagcagg gggccgcgaa tgataactgg tttgaaccgg
     5461 aggctgcgta aatgaactat gccggacacg aaaaacttcg cgccgacgtg gcggaggtgg
     5521 ccaacaccat gtgtgacctt cgggccaggc tgaacgatat ggagcatcgc tgtcgctttg
     5581 attccgatgt gcttgttgaa cgcctgacac gccagacgct gtatcgcgcc aatcgcctgt
     5641 tcatggaggc gtataccgaa attctggaac tggattcctg cttcaaagat tgagaaggag
     5701 acgagaatgt acggaacatg cgaaacgcta tgccgggagc tggcagcaaa gtatccggga
     5761 gacacgccac tgatgctggt tatctggtcc ccggaagaaa ttcaggccct ctctgacgga
     5821 atggatattg ccctgaccga tcatgaaatc aggactgtcc tggcgcgcct ggaggacatc
     5881 ccggaagacc agcggactga atccggtatt tcttccggcg tggcgatgga gatcatcaat
     5941 aacgtgagcg aaaaccgcca ggtgaccgtc cctgctgaac tgctggcgtc cctgattcag
     6001 accgctgaac aggcattgtg gaaacgtgaa tgggccgccc gggatcatgg cctcgccgtc
     6061 ccggaatgcg tcacccgccg tcaggcggtg gttaatcagg tccgcatcct gctgaaaaac
     6121 aacacacacg aaaacgactg atgttatcgc cgcctccggg cggcgataca gggagttcat
     6181 gaattatgaa tgaaacgctg aatgcactga tttatcgcca cgccagcaac ctgttgctgg
     6241 cgcagggctg gccggaagag acggatgttg accagcgtaa cccgaagtat ccgggctgga
     6301 tcagcattta cgtgcggctg gatgcgcccc ggctggcgac gttacttatc aaccgtcacg
     6361 gcggcgtact gccgccgctc cttgcctctg ccattcagaa actgaccgga accggagcgg
     6421 aacttgttct gtccggcagt cagtggcagt cgctgccggt acttccggca gacggaacgc
     6481 aggtgtcttt cccgtatgcc ggtgagtggc tggcagagga cgaaatcagg gccgttcttg
     6541 gtgcggtacg cgatgcagta tgcagtgtca gttaccaggt ggcagaagat gcgcggcgta
     6601 tccgtgcggc gctgaccaca accggtcaga cgttgctgac ccgccagacg cgccgctttc
     6661 gcctggtggt gaaggaaagc gatcacccct gctggctcga tgaagacgac gaaaacctgc
     6721 ccgtggtgct cgatgccatc ctgaaccggg gcgctcgttt ttcgtcggtg gaaatgtatc
     6781 tggtcagtga gtgtgtggag catatcctgt ccagtgggct ggcctgcgat atgctgcgta
     6841 taccggatga accgccccgc cgctggtttg accgtggtgt tctgcgggag gtggtccggg
     6901 aagcccggaa cgaaatccgc agcatggcgg atgccctggc aaaaatccgg aaatgatgac
     6961 gctggcgggg aaaaatctcc gccttttcca gccggtcccc ggaaagagca aaaggatgtc
     7021 gcctcaggct ccgctttacc cggccatgcg gggcatggcc ttgtgggttt tcagttatgt
     7081 ggcctcagcg tcgtgtgcgg gctgtgccgt gcctccatcg tagccgggct tctgcgggcg
     7141 ggttgcaagg taaggtgaac cgctgcggtc acccggtccc tccttcccgt ctgccgtgat
     7201 tttccggtcg ttcacccgct cagatttcgc ggtctcaccc tccaactccc gtcagccgtg
     7261 cgcgtcaggc tgcggcttcc cttgcgccct tgcaaccctg caggcccggc aaagatggag
     7321 gcacggcacc gcccggtgtc gcggaatatg tgaacaacgg aggaaatccc atgcagtatg
     7381 cgaaacctgt cactctgaac gttgaagagt gcgaccgtct gtcttttctg ccttacctgt
     7441 ttggcctgga ttttctgtat gccgaagcgt ctgtatacgc gctggcgaaa aaaatgatgc
     7501 cggaatatga aggcggattc tggcacttca tccgtctgcc ggacggtggc ggttacatga
     7561 tgccggatgg cgaccgtttc cacatggtga acggtgcaaa ctggtttgac cgtaccgtga
     7621 gtgctgatgc cgcaggcatc atccttacct cccttgtgat taaccgccag ttgtggctgt
     7681 accacgacag cggggatgca gggttgacac acctgtacag gatgcgcgat gcgcagttgt
     7741 ggagccacat cgaatttcac cccgaatgca acgcgattta cgcggcactt gactgattaa
     7801 cggacggggc ggcaatgccg cccctgtaaa ggagacgaga acatgtactg tactgttaaa
     7861 gaaatcatcc gcgatgtact ggatacagac gtgccggaca gtgaatgcgt ttttgccgtg
     7921 gtgctgaccc gtggggatgt gcgccacata gcccaggact ggagtctgac agacgatgag
     7981 ctggaaaccg tcatgcagcg gctggacgat gcttttgtat atggcgcatg tgacagagtt
     8041 gtttccgata ttgtcaatga actgatggag gaaaagcgcg taaaccgtct tgtaacagtc
     8101 cctgcggtgc tgctggaaaa agtgatggtg atggctggta gtgaaattta tcgcctccat
     8161 gctgtaggga gcgagaacgg gggcgacggt gacgcatttg tcagggaaga acgtgagatt
     8221 atgcgggtga tgcgtcaggc gctggacggg gagaacgggt aatgaatatc agcacggaaa
     8281 cccgcgaaat tctgcgtaat tacagggccg tgattaatgc gcggcgtcgt gaaatggggc
     8341 agaaaccgct caccaccgcg cagattgttg atgaaatctg cgattttgtg gcgaatcagc
     8401 aggcggtttt cctcggtggt cactacatcc ttcagggcag caggcacagg tgatacaggg
     8461 caggcggtgg aggccgcctg tcagcccgcg cgaaaacccc tggcaggctg gcgcctgccg
     8521 gaagggatgc gggccacgct ggcgcgtcgc ccgttaccct tcccgtttgt gatgtgtctg
     8581 actcaggagt gtttctttct tatgttatgg aactgttcct gatgttgttg ttgttctttt
     8641 ctctgctgct ctgattctga ctactgcagt gttgtcctgt ctgccgggcg caccttcggc
     8701 tcccggctgc ctggcggcat ccggtcgtca ggcccgactc cgacaggcag ctgctggcgc
     8761 acccgccagt ctccgccgaa cccgctggcg cggtttcggg ctgtcgctac ggtccgatgc
     8821 gtgaaaaccc ctggcttgca gcgaccggaa cggtcgcgcc gcccttgtcg cgccgtggcc
     8881 tgcgctcctg gcatcccggc aaaaagacgc cgggatgtaa ggagcaacgc cgtttcgcaa
     8941 accgcgcttc acgtcgttgc ggcgggtccc tgctcattgc ctgccgggtg ccacacaccc
     9001 gtatgagtcc ctgtcgccgg gggctgatac agccacaacc tgccgcactg actgtccagg
     9061 gtaaatgaac agcccacaaa attttttacc cagaacccgg taaaaaattt tgcgttctgc
     9121 ccaggacagg tgcgtcaggc cgtggcagtg atgccccttg cggcgaaggt tctcaaacgg
     9181 gtgaagttca taccacaggg aatacagcag caggatcccg cgaaacgtca aaaccgcggc
     9241 tcccgaaact caacgggtga tgttctttaa aaatcaggat ttatttcagg ctccggcaat
     9301 acgcatttat gtgttctgtc agcatcctgc tggcaggtaa tgagaacgca ggaacagaag
     9361 gaggaataca ccggaaaacg tcagatgcag atatgaaaaa agcaggtcag ggacctgctt
     9421 aatccacgcc gaagagactg tgcaaccagt ctttaaggca ctgttaccaa cctgtcataa
     9481 ggtatccatt tatgactgta agcattgttt caccttccgc agcggctgta aagccccgcc
     9541 gtcatccccg tttcagacgc gaagacatcc ccgcgccgga aattgacccc gtgctgaaag
     9601 ccttcgggcg acacatagcc cggagttttc accgggggcg tggtgtacac atccccgcca
     9661 tgaaaaacac ggctttcggg caggtgctcc gcacactcga actgaaacgc gcttttaact
     9721 gatgaggtga gccgccccgc gaggggcggc aacagacgga aggagaacat tttatgacat
     9781 tagcagcaat caccatgacc gccccggaag ccgccagccc tgtgcagatg taccgcgcga
     9841 cctactcacc ggatgacaac aaactgcgcc tgtatgccgc gtcacgtctt gacccggaga
     9901 cgtataaaaa agtacatgat gccggtttcc gctgggcacc agaacaggcg ctgtttgtcg
     9961 cgccagcctg gacaccgggc cgggaagacg tgcttctctc acttgccgga gagattgagg
    10021 atgaagacag tacgctcgct gaacgtcagg aagcacgggc ggagcggttt accggataca
    10081 gcggaaagcg ggccagtgaa tccgcacagg cacttgatga agtggaaaga ctggccgcga
    10141 tgatcccgcc cggtcagccc attcttgtgg ggcatcacag cgaacgccgc gcccgtcgtg
    10201 atgcacagcg tattgaaaac ggtatgaaac gtgctgtgat gctctttgaa cgtgcggaat
    10261 actgggaaga gcgggcgcgg tcggcactgc ttcacgcgaa gtataaagaa cgtccggacg
    10321 ttcgctggcg tcgtatcaaa aaaatcgaag ctgatttgcg caaggcggaa aagaccatcg
    10381 cacagtcgca gaaatatctg acgatgtggc gggctgaatc gctggatctg aatatggcaa
    10441 aactcatcag cagtcatgac catatcagcg cctgtttccc gctggacagg tatccgcgcc
    10501 cggcagaaaa aagccagtat gaaggaagtc gatcgttatg gtcggccctg gatgatgaca
    10561 tcatcaccac ggagcaggcc cgcgaaattg cgatccgctg tcatgaacgg cagattcagc
    10621 gtcagcaacg ctgggttaac cactatcaga accgcctgat ctatgagcgt gccatgctgg
    10681 acgaaagcgg cggcgtggtt acccggacac aggattttga gccgggcggg caggttttca
    10741 gccggggcga gtggctgacc atcatccgcg tgaacaaaag caacggggcg gtgagttcag
    10801 tcacaacgcc gaattacagt tttctcgggt acagcggcac gatgaaagtg acgcccgatc
    10861 gcatcacgga ctacaaagca ccatcggcag aagaggctgc cgtcgccagt caggccgcga
    10921 agcgtccgcc ggtagtcaac tatccggggg aaggtttccg ggaaatgaca aaggcgcagt
    10981 gggccgccct gccccgggac tgtaaggccg tgcgcagtgt ggcagagaca gaagaccacg
    11041 gggcataccg ctaccgccgc acaatggaca ataatttccg cctggtgaat gtgtatatca
    11101 ccgacatgaa aatcacggaa atcccacaga aataaggtat atccccccgg gaaatccccg
    11161 ggggatgacg gagataatga catgcacagt cagttaagag aacgtatccg gctgatgcgc
    11221 gcaaggctgg ataacgccgc gccggttgct gaaatccggg ctgaatctca gctttttgtg
    11281 actcctgccc cggtctgtga tcgcctggtg acgctggcgg aaatcagcaa ccgtgatcac
    11341 attctggaac cctctgccgg caccggggcc atactgcggg cgattcggga taccgcgccg
    11401 gaggccatgt gtgatgcggt ggagattaac agcgggctgg tccggtatct gcgggaaaat
    11461 tttaacggtg tcagggtaca gtgtggcgac ttcatggaat ggctgccggt gcagtattac
    11521 agccggatca ttatgaatcc gccgttcagt cacgggcagg atatccggca tatcctgcgg
    11581 gctttttccc tgttgcgtcc ggggggcgtt ctggttgccg tctgtctcaa cgggccacgc
    11641 cagcaggaga agctgttacc gttttctgac gtccgcgagg agctgccgcg cggcacgttt
    11701 gcttataccg atgtcccgac gatgattatt cgtttgcgtg cctgatgaca cagggctggc
    11761 gggaggataa atgcccgtct gtccgcgccg ggaaaaaacc gcgcgccggt ggcgctgaaa
    11821 ggggccgcgc tcacgcgccg cccctttcat tacgcggacg ttctgatggg ggcttctgtc
    11881 ttttcagacc tgtcatttgt ctgttactga tgatgttgtt gttcctgcta ctgctactgc
    11941 tactgctact gctactgcta ctgctcttct ttttcctgcc gctctgattc tgactactgc
    12001 agtgttgtcc tgtctgccgg gcgccccttc ggctcccggc tgcctggcgg catccggtcg
    12061 tcaggcccga ctccgacagg cagctgctgg cgcacctgcc agtctccgcc gaacccgctg
    12121 gcgcggtttc gggctgtcgc tacggtccga tgcgtgaaaa ccccgggttt gcagcgaccg
    12181 ttccggtcgc gccgcctctg tcgcgccgtg gcctgcgctc ctggcatccc ggcaaaaggc
    12241 cgccgggatg taaggcgcaa cgccgtttcg caaaccgcgc ttcacgtcgt tgcggcgggt
    12301 ccctgcccgt tgttgtgtcc ggtgccattc acctgtttaa gcccctgtcg ccgggggctg
    12361 atacagccac aacctgccgc actgactgtc cagggtaaat gaacagccca caaaattttt
    12421 tacccaaaac ccggtaaaaa attttgcgtt ctgcccagga caggtgcgtc aggccgtggc
    12481 agtgatgccc cttgcggcga aggttctcaa acaggtgatg gtgaataccg gagggaacaa
    12541 agagcaggtc cccgcaaaac gtcaaaaccg cggctcccga aactcaacag atgatggggg
    12601 ctgaaaacat caacagaagg agacatcatg gcagttcgtg gcattaacaa ggtcattctc
    12661 gtgggacgtc tgggaaaaga tcctgatgtc cgttacatcc ccaacggggg cgcagtggca
    12721 aatctgcagg tggccacgtc agaaagctgg cgtgacaaac agacggggga gatgcgggag
    12781 cagacagaat ggcaccgcgt ggtgctgttc ggcaagctcg cggaagtggc aggtgaatat
    12841 ctgcgtaagg gcgcgcaggt ctacatcgag gggcaactcc gtacccgtag ctgggaagat
    12901 aacggtatta cccgttacgt cactgaaatc cttgtgaaaa ccacgggcac catgcagatg
    12961 ctggggagtg caccacagca gaacgctcag gcgcaaccgg agcctcagca gaatgggcag
    13021 ccacagagtg ctgacgcgac gaaaaaaggt ggcgcgaaaa cgaaaggccg tggacgtaag
    13081 gccgcgcagc cggagcctca gcagccgtct gaacccgcgt atgacttcga tgacgatatt
    13141 ccgttctgaa cgggctgact gtgataaccg ccccgtcctg cgcggggcat caccggagaa
    13201 atgaggatga gcgaatattt cagaatactt cagggactgc cggacggctc ctttacccgc
    13261 gaacaggcgg aagccgttgc cgcacagtac cggaacgtct ttatcgagga tgatcaggga
    13321 acgcattttc gcctggttgt ccgtcaggat ggcacgttga tctggcgctc ctggaatttt
    13381 gaggactgtg ccgggtactg gatgaaccgg tacatcaggg atttcgggat ccttaagtaa
    13441 gagaggtgcc ggacggggat cacgtccggc agacattaac tacacaataa ggggatagtc
    13501 atgtcagtaa caaagtctga accaaaatct acccgtaaag caagccgtaa acttgcaaaa
    13561 acgcaggaaa ctgtcctgtc tgccctgctg gcgcagacgg aggaagtgag cgtgccgctg
    13621 gcctcactga ttaaatcacc gctgaatgtg cgcacggtgc cgtattctgc ggagtccgtc
    13681 agtgaactgg ctgattccat taagggagtc ggcctgctgc agaatctggt cgtgcatacc
    13741 ctgcctggtg accgttacgg tgtcgccgca ggtggtcgcc gactggctgc actcaacatg
    13801 ctggcagagc gtggcatcat tccggctgac tggcctgtct gtgtgaaggt cattccgcag
    13861 gaactggcga cggcggcatc aatgactgaa aacggtcatc gtcgggatat gcaccctgct
    13921 gaacagattg ccggattccg cgcaatggcg caggaaggca aaacacctgc acaaatcggt
    13981 gatttgctgg gctattcacc ccgccacgtt cagcgaatgc tgaaactggc agaccttgca
    14041 ccagtcatac tggatgcgct ggcagaagac cgcatcacca ccgaacactg tcaggcgctg
    14101 gcactggaga acgacaccgc gcgtcaggtg caggtgtttg aagccgcctg tcagtcggga
    14161 tggggcggta aaccggaagt acagaccatt cgtcgtctgg tgaccgaaag tgaagtggcg
    14221 gtggcaggca acagtaaatt ccgcttcgtg ggggctgatg ctttctcacc tgacgaactg
    14281 cgcaccgatt tgttcagcga cgacgagggt ggctatgtgg actgtgttgc gctcgatgct
    14341 gccctgctgg aaaaactcca ggctgtcgct gaacaccttc gggaagctga aggctgggaa
    14401 tggtgcgccg gacgtatgga gtctgtcggt ttctgccgtg aggatgccgg aacataccgt
    14461 tgtctgccgg agccggaatc ggtgctgacc gaggcggaag aagaacgcct gaacgaactg
    14521 atggcacgtt acgacgcact ggaaaaccag tgtgaggaat ctgacctgct ggcagcagaa
    14581 atgaagctga ttgactgcat ggcgaaggtc agggcgtgga cgccggagat gcgtgccgga
    14641 agtggcgtgg tggtgtcctg gcgttatggc aacgtatgtg tccagcgtgg tgtgcagttg
    14701 cgcagcgagg atgacccggc tgccgacgct gaccgcacgg aacaggtgca ggagaaagcg
    14761 tcagtggagg aaatcagtct gccactgctg acgaaaatgt cttcagagcg cacgctggca
    14821 gtccaggcgg cgctgatgca acagccggac aaatccctgg cactgctggc atggacgctc
    14881 tgcctgaatg tgtttggcag cggggcgtac agtaaaccag cacaaatcag cctggaatgt
    14941 gaacattatt cgctgaccag cgatgcgcca tcggggaagg aaggtgccgc attcatggcg
    15001 ctgatggcag aaaaagcccg tcttgctgcc ctgttacctg tgggatggtc acgggacatg
    15061 acgacattcc tgtcactcag tcaggaggtg ctgttatccc tgctcagttt ctgcaccgca
    15121 tgcagcctta acggtgtcca gacccgtgag tgtggtcaca cgtcacgcag tccgcttgac
    15181 tcgctggaga gcgccatcgg ctttcacatg cgtgactggt ggcagccgac gaaagcaaac
    15241 ttcttcggac acctgaaaaa gccgcagatt atcgcagccc tgaatgatgc cggactgtcc
    15301 ggtgccgcac gggacgcgga gaagatgaag aaaggcgatg ccgctgaaca tgcagagttc
    15361 cacatgaaag acaaccgctg ggtgcctggc tggatgggtt caccacgtcc acagacggat
    15421 gccactgaac gcgccgataa cctggctgat gccgcctgat gaacaaccac accgccccgc
    15481 cggagacggg gcggcagcaa gggagatacc gtgatgaaaa ctgaactgac cctggatgta
    15541 ttacagacca tgagcgcaca ggaatatgaa gatatccggg ctgccggaag tggtgaacgt
    15601 cgtgagctga cacacgccgt gatgcgggag ctggatgcgc cggataactg gacgatgaac
    15661 ggcgagtacg gcagcgagtt tggcggcttt ttccccgtcc aggtccgttt cacgcctgcc
    15721 cacgaacgtt ttcacctggc gttatgttca ccgggtgacg tctctcaggt ctgggtgctg
    15781 gttctggtga atgccggcgg tgagccgttt gcggtggttc aggtgcagcg gcggtttgta
    15841 ccggaagccg tcagccattc actggcactg gcggcgtcac tggatgcgca ggggtacagc
    15901 gtcagtgaca tcatccatat cctgatggcg gaaggaggtc aggcatgagc gtacgttcac
    15961 aggcactggt tcccctcagc gcagagcaac aggccgcatg gcgggcggtg gcggagacgg
    16021 aaaaacgccg ccatcaggga aacacgctgg cggaatatcc gtatgcgggg gcgtttttcc
    16081 gctgtctgaa cggaagccgc tggatttcgc tgtctgacct gcgatttttc atgccgtccc
    16141 tgaccgcaga agaactgcac ggcaaccgtc ttcagtggct gtatgccatc gatgtgctga
    16201 ttgaaacaca gggcgaggtc tgcctcctcc cccttcccgg cgatgcagca gaacgcttgt
    16261 tcccgtcggt gcggttccgt gtcagggaac gccgtcgcca taaatctgcg ctggtcatgc
    16321 agaagtacag ccgccagcag gcgagggagg cagagcagaa aacccgtgcg taccaggcgc
    16381 tggttgctca ggcagaaatc gaactggcgt tccattcacc ggaaacagtc ggaagctggc
    16441 atgcacgctg gagtgaccgg gtggcagagc atgacctgga aaccctgttc tggcagtggg
    16501 gcgagcgttt tccgtcactg gccggaatgg aacgctggca gtggcaggat atgccgttct
    16561 ggcaggtcat cgcagaggca ggtatggcgg caagggaggc cggtcatgcc gtccgtgaga
    16621 tggagcgatg gatggtgccg aacaaactcc gggaggcggc gtgaagtggc gttactcact
    16681 gcgctggaaa ctgccttatc cctgtcctgg cgagcatgaa ctggtttcag aagtggtgga
    16741 ggcggggcag cctgcaccgg catccgttat gtcacgctgg gttgccggag cagggtatgc
    16801 ggtctgtctg gattttattt ctgaccgccc ggtgcgccgc tggagcgaag aacgtaaggc
    16861 tgcggtacgg cggcggaatc tggagaaacg tatcaacagg catgcaccac tgtttgcgga
    16921 tgaactgatt gcccgtgaac tggctgaacg accggattat tttcagggaa agtgagtgtg
    16981 gtcagtacgc aggtatatgg gctatgatgt gcccggcgct tgaggctttc tgcctcatga
    17041 cgtgaaggtg gtttgttatc ctgtcgtgtg gcagaaggac aaaagccccg tagttaattt
    17101 ttcattaacc cacgaggcct ctgcatgtct agtccacgtc agtatggcct cttaccgtgc
    17161 aaattgcaag gaggagtaaa ccatgaaact accgggaaac gcccttatct ggtgcgtatt
    17221 aatcgtgtgc tgcacgttgt tgatattcac actcctgacc cggaatcgcc tgtgcgaagt
    17281 ccggctgaag gacggataca gggaagttac ggcaacaatg gcttacgaat ccggcggtaa
    17341 gtagcaacct ggaggcgggc gcaggcccgc cttttcaggg ctgatgctgg tctgactgca
    17401 ctgaagcgcc tttataaagg ggctgctggt tcgccggtgg cccctttctc cttgctggca
    17461 ttgtagcagc atgaacaacc agactgtcag gaaggccact tccctccggg aacgggaatg
    17521 aacgtgcgcc tgcggcgctc gttcttttcc cccgccttct ctgtttatgg ccagttgtca
    17581 gaaatgacat tacctttccc gtgattattc tctttttctt cctgctctga ttctgactac
    17641 tgcagtgttg tcctgtctgc cgggcgcacc ttcggctccc ggctgcctgg cggcatccgg
    17701 tcgtcaggcc cgactccgac aggcagctgc tggcgcacct gccagtctcc gccgaacccg
    17761 ctggcgcggt ttcgggctgc cgctacggtc cgatgcgtga aaaccccggg cttgcagcga
    17821 ccgttccggt cgcgccgcct ctgtcgcgcc gtggcctgcg ctcccggcat cccggcaaaa
    17881 agacgccggg atgtaaggcg caacgccgtt tcgcaaaccg cgcttcacgt cgttgcggcg
    17941 ggtccctgcc cgttgttgtg tccggtgcca ttcacctgtt taagcccctg tcgccggggg
    18001 ctgatacagc cacaacctgc cgcactgact gtccagggta aatgaacagc ccacaaaatt
    18061 ttttacccaa aacccggtaa aaattttgcg ttctgcccag gacaggtgcg tcaggccgtg
    18121 gcagtgatgc cccatgcggc gaaggttctc aaacaggtga tggtgaatac cggagggaac
    18181 aaaaggcagg tccccgcaaa acgtcaaaac cgcggctccc gaaactcaac agatgatggg
    18241 ggctgaaaac atcagataca ggaggaaaca tgagcgcatt ttcttttaac accgcttatc
    18301 agccaacagg cgatcagcag aaagacgcaa tcgcacaaat cgacatcatg cagaatcgtg
    18361 ctgttcaggc aaatcttgca taccagagca gcctggatgc ggaaacgcta ttgaaacagt
    18421 tatggcaaat caatgatgaa ctgggggaac tgttggatag cgtggaaaat cacacgccag
    18481 ttatcagaaa aaaagccagt gatttgagcg cactgatgat gcttttcagc caacaggccg
    18541 gtcagcctat gactatgttg ttgatttgtt aatgataatc aggggcagga aattaagcga
    18601 aagatgctct gccccgggtt tgcagcgacc ggaacggtca cgccaccctt gtcgcgctgt
    18661 ggcctgcggc ggctcccgaa actcaaccga tgatgggggc tgaaaaccag agagaggaga
    18721 aagcaggatg agtacccgaa atatccacgt taacaccgcg tcgtacacgc ttcttgttgc
    18781 cgggaagaaa aaaaacaccg gggaagagtg ggacgtcctg gaattcagca gcctgactga
    18841 gctgaaaaaa tatcgcaaaa gccacccgga aaagatggcc ttcagctata gctacgcgct
    18901 cagccggggt gtggatacgc agttccgcca tatcaacatt gcggaagccg atcacttcaa
    18961 acagttcctg cgtcagatta agcgtaccgg cctcgatatc cgggcgatct gctgaccgga
    19021 tgtgatgaat atccctcctg attgttgcaa tcttgttaat ggggaccggg agggatgata
    19081 ttaagcggaa aaatttttta ttaaatttat tctttcataa aggagtagct gttatgcgat
    19141 tagcttcccg ttttggtcgg tataattcca ttcgccgtga gcgtccttta acggatgatg
    19201 aattaatgca gtttgtgcct tcggtatttt ccggtgataa acatgaatcc cggagtgagc
    19261 gttatacata tattccaaca atcaatatca tcaataagtt acgtgaagaa ggtttccagc
    19321 cgttctttgc atgtcagagt cgggttcggg atttgggacg ccgcgaatac agtaaacata
    19381 tgttacgtct tcgcagggaa gggcatatta acgggcagga agttcctgaa attatcctgc
    19441 ttaattcaca tgatggttca tccagttatc agatgatccc aggaattttt cgttttgtct
    19501 gcacaaatgg tctggtgtgc ggaaataatt ttggcgaaat ccgcgttcca cataaaggtg
    19561 atattgtcgg gcaggttatc gagggtgcgt atgaagtgcc cggtgtcttt gataaggtca
    19621 ctgataatat ggaggcgatg aaagaaatac atcttaacag tgacgagcaa catttatttg
    19681 gcagagctgc actgatggtc aggtatgaag atgaaaataa aacaccggtg acgcctgaac
    19741 aaataattac tccccgtcgt tgggaggata aaaagaacga tctctggaca acctggcagc
    19801 gggttcagga gaatatgata aaaggtggac tgtcggggcg aagtgcctcc ggtaaaaata
    19861 ccaggacaag aggcattacg ggtattgatg gtgatatccg aatcaacaaa gcattatgga
    19921 tgattgccga acagttcagg gagtggaagt catgataata taagtaaagc cccgaaaatt
    19981 ttttcggggc tttacttata ttatcattat gcggcattga tatgtcgtct gagatgcctc
    20041 aatttcatca actggctttt cggtagtctg taaatattgt gcccgcgaaa aatggtctgg
    20101 caggaggctg cgcagttacc ggatatatat aagatgggtt cccgctttgc ggggcttcgg
    20161 ccagtcagca ttatcactca tatgatattt ttgtgtgtgg ccttccatgc cgcgtttgcg
    20221 gcataaccgg ttttttaaaa tgtatatcaa attaattctt ctgcgagctg ttcatttttg
    20281 aaagcggttt ttcagatgca cgaagctgga tccccttgct gttttttatc ccgctgtaaa
    20341 tccggtaaac atctgatgca taggcaagac gccgctggtt ctggcgttcg gttttcctga
    20401 atcctgcgtt gtatgcacca acagcctccc agaagactcc ccatttttta aaggctatag
    20461 ccagataata agcaccggta taaatgttca gacagggatc tgctgtcaga tgttccggtt
    20521 taattccgta gcgggccagt tcgttaaaat gctgggaatc tacctgcata agtccactgc
    20581 catatcccgt aaccggatta ataccgatgg cattaacccg gttacgggat tctttccatg
    20641 atattgctct cagcaagtca gggtcaattt tataatctct gccagccaga tcaaagcaat
    20701 caccagcctt gcaggtcaca tttataaaca tcaggcagat cgtcagaagc cattttctca
    20761 ttttctcacc tctggtgacg tcatcaggaa ataatttaac ccactccaca aaaaggctca
    20821 acaggttggt ggttctcacc accaaaagca ccacacacta cgcaaaaaca agtttttgct
    20881 gattttcgtt taatatcatt gagttgtatt tgcggattta tattgtttaa atctgattta
    20941 tttaaaacag cgtcgttaac gccgctacag caacgcgcag acaccgcttt ttaggggtgg
    21001 tactgactat ttttataaaa aacattattt tatattaggg gtgctgctag cggcgcggtg
    21061 tattttttta taggataccg ccaggggcgc tgctagcggt gcgtccctgt ttgcattatg
    21121 aactctggtg tttctaaatt aactttattt tatgttcaaa aaaggtaatt tctaatggct
    21181 aaggtgaacc tgtatatcag caatgatgct tatgaaaaaa taaatgcgat tattgagaag
    21241 cgtcgacagg aaggggcaag agaaaaagat gtcagttttt cagcaacagc ttcaatgctt
    21301 cttgaactgg ggcttcgtgt atatgaagct cagatggagc gtaaagagtc cgcgtttaat
    21361 cagattgagt ttaataaatt acttcttgaa tgcgttgtaa aaacacaatc atcagtagcg
    21421 aaaattttgg gtattgagtc tctcagtcct catgtttccg ggaatccaaa gtttgaatat
    21481 gccaatatgg ttgaagatat ccgggagaag gtgtcatctg agatggaacg a
//
LOCUS       DAZKFU020000064        21424 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZKFU020000064 DAZKFU020000000
VERSION     DAZKFU020000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.064"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1080
                     /locus_tag="ACHOQQ_RS09435"
                     /old_locus_tag="pgaptmp_001887"
     CDS             <1..1080
                     /locus_tag="ACHOQQ_RS09435"
                     /old_locus_tag="pgaptmp_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HGU4470146.1"
                     /translation="PLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGL
                     KSLPEGWINIAERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPD
                     EWQVYMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVIL
                     SHEHLDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASN
                     DKGNNHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQATLLQVPHHGSNTSSS
                     LPLIQRVNGKVALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQLTVNFSAQGW
                     LISSLREQILPRWYHQWFGVPVDNG"
     gene            1117..2865
                     /gene="msbA"
                     /locus_tag="ACHOQQ_RS09440"
                     /old_locus_tag="pgaptmp_001888"
     CDS             1117..2865
                     /gene="msbA"
                     /locus_tag="ACHOQQ_RS09440"
                     /old_locus_tag="pgaptmp_001888"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGU4470147.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            2862..3848
                     /gene="lpxK"
                     /locus_tag="ACHOQQ_RS09445"
                     /old_locus_tag="pgaptmp_001889"
     CDS             2862..3848
                     /gene="lpxK"
                     /locus_tag="ACHOQQ_RS09445"
                     /old_locus_tag="pgaptmp_001889"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGU4470148.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVVDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            3885..5117
                     /gene="ycaQ"
                     /locus_tag="ACHOQQ_RS09450"
                     /old_locus_tag="pgaptmp_001890"
     CDS             3885..5117
                     /gene="ycaQ"
                     /locus_tag="ACHOQQ_RS09450"
                     /old_locus_tag="pgaptmp_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGU4470149.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            5169..5351
                     /gene="ycaR"
                     /locus_tag="ACHOQQ_RS09455"
                     /old_locus_tag="pgaptmp_001891"
     CDS             5169..5351
                     /gene="ycaR"
                     /locus_tag="ACHOQQ_RS09455"
                     /old_locus_tag="pgaptmp_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGU4470150.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            5348..6094
                     /gene="kdsB"
                     /locus_tag="ACHOQQ_RS09460"
                     /old_locus_tag="pgaptmp_001892"
     CDS             5348..6094
                     /gene="kdsB"
                     /locus_tag="ACHOQQ_RS09460"
                     /old_locus_tag="pgaptmp_001892"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGU4470151.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLKRVRAEMR"
     gene            6248..7141
                     /locus_tag="ACHOQQ_RS09465"
                     /old_locus_tag="pgaptmp_001893"
     CDS             6248..7141
                     /locus_tag="ACHOQQ_RS09465"
                     /old_locus_tag="pgaptmp_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGU4470152.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(7118..7897)
                     /gene="elyC"
                     /locus_tag="ACHOQQ_RS09470"
                     /old_locus_tag="pgaptmp_001894"
     CDS             complement(7118..7897)
                     /gene="elyC"
                     /locus_tag="ACHOQQ_RS09470"
                     /old_locus_tag="pgaptmp_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGU4470153.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLSLLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRDNPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            8033..8818
                     /gene="cmoM"
                     /locus_tag="ACHOQQ_RS09475"
                     /old_locus_tag="pgaptmp_001895"
     CDS             8033..8818
                     /gene="cmoM"
                     /locus_tag="ACHOQQ_RS09475"
                     /old_locus_tag="pgaptmp_001895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGU4470154.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            8815..10137
                     /gene="mukF"
                     /locus_tag="ACHOQQ_RS09480"
                     /old_locus_tag="pgaptmp_001896"
     CDS             8815..10137
                     /gene="mukF"
                     /locus_tag="ACHOQQ_RS09480"
                     /old_locus_tag="pgaptmp_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGU4470155.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            10118..10822
                     /gene="mukE"
                     /locus_tag="ACHOQQ_RS09485"
                     /old_locus_tag="pgaptmp_001897"
     CDS             10118..10822
                     /gene="mukE"
                     /locus_tag="ACHOQQ_RS09485"
                     /old_locus_tag="pgaptmp_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGU4470156.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            10822..15282
                     /gene="mukB"
                     /locus_tag="ACHOQQ_RS09490"
                     /old_locus_tag="pgaptmp_001898"
     CDS             10822..15282
                     /gene="mukB"
                     /locus_tag="ACHOQQ_RS09490"
                     /old_locus_tag="pgaptmp_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGU4470157.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVEQ
                     RHLADQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNNDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            15543..17390
                     /gene="ldtD"
                     /locus_tag="ACHOQQ_RS09495"
                     /old_locus_tag="pgaptmp_001899"
     CDS             15543..17390
                     /gene="ldtD"
                     /locus_tag="ACHOQQ_RS09495"
                     /old_locus_tag="pgaptmp_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4470158.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            17571..18119
                     /locus_tag="ACHOQQ_RS09500"
                     /old_locus_tag="pgaptmp_001900"
     CDS             17571..18119
                     /locus_tag="ACHOQQ_RS09500"
                     /old_locus_tag="pgaptmp_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGU4470159.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            18146..18793
                     /gene="gloC"
                     /locus_tag="ACHOQQ_RS09505"
                     /old_locus_tag="pgaptmp_001901"
     CDS             18146..18793
                     /gene="gloC"
                     /locus_tag="ACHOQQ_RS09505"
                     /old_locus_tag="pgaptmp_001901"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGU4470160.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLKEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(18844..20034)
                     /gene="aspC"
                     /locus_tag="ACHOQQ_RS09510"
                     /old_locus_tag="pgaptmp_001902"
     CDS             complement(18844..20034)
                     /gene="aspC"
                     /locus_tag="ACHOQQ_RS09510"
                     /old_locus_tag="pgaptmp_001902"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGU4470161.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(20219..21292)
                     /gene="ompF"
                     /locus_tag="ACHOQQ_RS09515"
                     /old_locus_tag="pgaptmp_001903"
     CDS             complement(20219..21292)
                     /gene="ompF"
                     /locus_tag="ACHOQQ_RS09515"
                     /old_locus_tag="pgaptmp_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGU4470162.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNK
                     TRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAAD
                     RTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPIEGGFANKTQDVLL
                     VAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQ
                     IDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         5113 a         5309 c         5883 g         5119 t
ORIGIN      
        1 cccttggtga catttatcac ggttccgctg atcctcgccg cgatggttgt gcatttaagc
       61 gggccgttaa tcctggagca agggttatgg tttcttgccg accggtcttt ggctttactt
      121 ttctgggggt taaagagttt gccagaaggg tggatcaaca ttgctgaacg ttggcaatgg
      181 ctatcatttt ccccatggtt cttactggtg gtatggcgat taaacgcctg gcgaacgttg
      241 ccagcaatgt gtgtggctgt aggcttgctg atgtgctggc cgctgtggca aaaacctcga
      301 cctgacgagt ggcaagtgta catgcttgat gtcgggcaag ggctggcaat ggtgatagcc
      361 agaaacggca aagcgattct ctatgacacg ggactggcct ggcccgaagg ggatagtggg
      421 caacaactga ttatcccctg gctccactgg cataatcttg aaccggaagg cgttattctg
      481 agtcatgaac atctggatca ccggggaggg ctggactcaa tattgcacac atggccgatg
      541 ttatggatca gaagtccgtt aaactgggaa catcatcagc cctgtgtgcg tggcgaagcg
      601 tggcaatggc aaggattgcg tttcagcgtg cactggcctt tacaagctag caacgataaa
      661 ggaaataacc attcctgtgt ggttaaggtt gatgacggga cgaatagcat tcttctaacc
      721 ggtgatattg aagtccccgc tgaacaaaag atgctaagcc gttactggca gcaagtgcag
      781 gcaacattgc ttcaggtacc tcaccatggc agtaatacct catcatcgtt gccattaatt
      841 cagcgagtga atggaaaagt ggcactcgca tcggcatcgc gctataacgc atggcgactg
      901 ccctctaaca aagttaagca tcgctatcaa caacagggtt atacgtggct tgatactcct
      961 catcaggggc aactaacggt caatttttca gcacaaggct ggctgattag cagcctcagg
     1021 gagcaaattt tacctcgttg gtatcatcag tggtttggcg tgccagtgga taacgggtag
     1081 aatatgcggc tatttcaaca aatgctggtt ttttgaatgc ataacgacaa agatctctct
     1141 acgtggcaga cattccgccg actgtggcca accattgcgc cttttaaagc gggtctgatc
     1201 gtggcgggcg tagcgttaat cctcaacgca gccagcgata ccttcatgtt atcgctcctt
     1261 aagccacttc ttgatgatgg ctttggtaaa acagatcgct ccgtgctggt gtggatgccg
     1321 ctggtggtga tcgggctgat gattttgcgt ggtatcacca gctatgtctc cagctactgt
     1381 atctcctggg tatcaggaaa ggtggtaatg accatgcgtc gccgcctgtt tggtcacatg
     1441 atgggaatgc cggtttcatt ctttgacaaa cagtcaacgg gtacgctgtt gtcacgtatc
     1501 acctacgatt ccgaacaggt tgcttcttcc tcttccggcg cactgattac tgttgtgcgt
     1561 gaaggtgcgt cgatcatcgg cctgttcatc atgatgttct attacagttg gcaattgtcg
     1621 atcattttga ttgtgctggc accgattgtt tcgattgcga ttcgcgtagt atcgaagcgt
     1681 tttcgcaaca tcagtaaaaa catgcagaac accatggggc aggtgaccac cagcgctgaa
     1741 caaatgctga aaggccataa agaagtattg attttcggtg gtcaggaagt ggaaacgaaa
     1801 cgcttcgata aagtcagcaa ccgaatgcgt cttcagggga tgaaaatggt ttcagcctct
     1861 tccatctctg atccgatcat tcagctgatc gcctctttgg cgctggcgtt tgttctgtat
     1921 gcggcgagct tcccaagtgt catggatagc ctgactgccg gtacgattac cgttgttttc
     1981 tcttcaatga ttgcactgat gcgtccgctg aaatcgctga ctaacgttaa cgcccagttc
     2041 cagcgcggta tggcggcttg tcagacgctg tttaccattc tggacagtga gcaggagaaa
     2101 gacgaaggta agcgcgtgat cgagcgtgcg actggcgacg tggaattccg caacgtcacc
     2161 tttacttatc cgggacgtga cgtacccgca ttgcgtaaca tcaacctgaa aattccggca
     2221 gggaagacgg ttgctctggt tggacgctct ggttcaggta aatcaaccat cgccagcctg
     2281 atcacgcgtt tttacgatat tgatgaaggc gaaatcctga tggatggtca cgatctgcgc
     2341 gagtataccc tggcgtcgtt acgtaaccag gttgctctgg tgtcgcagaa tgtccatctg
     2401 tttaacgata cggttgctaa caacattgct tacgcacgga ctgaacagta cagccgtgag
     2461 caaattgaag aagcggcgcg tatggcctac gccatggact tcatcaataa gatggataac
     2521 ggtctcgata cagtgattgg tgaaaacggc gtgctgctct ctggcggtca gcgtcagcgt
     2581 attgctatcg ctcgagcctt gctgcgtgat agcccgattc tgattctgga tgaagctacc
     2641 tcggctctgg ataccgaatc cgaacgtgcg attcaggcgg cactggatga gttgcagaaa
     2701 aaccgtacct ctctggtgat tgcccaccgc ttgtctacca ttgaaaaggc agacgaaatc
     2761 gtggtcgttg aggatggtgt cattgtggaa cgcggtacgc ataacgattt gcttgagcac
     2821 cgcggcgttt acgcgcaact tcacaaaatg cagtttggcc aatgatcgaa aaaatctggt
     2881 ctggtgaatc ccctttgtgg cggctattgc tgccactctc ctggttgtat ggcctggtga
     2941 gtggcgcgat ccgtctttgc tataaactaa aactgaagcg cgcctggcgt gcccccgtac
     3001 cggttgtcgt ggttggtaat ctcaccgcag gcggcaacgg aaaaaccccg gtcgttgtct
     3061 ggctggtgga acagttgcaa cagcgcggta ttcgcgtggg ggtcgtgtcc cggggatatg
     3121 gtggtaaggc tgaatcttat ccgctgttat tgtcggcaga taccaccaca gcacaggcgg
     3181 gtgatgaacc tgtgttgatt tatcaacgca ctggcgcgcc cgttgcggtt tctcccgtgc
     3241 gttctgatgc ggtaaaagcc attctggcgc aacaccctga tgtgcagatc atcgtaaccg
     3301 acgacggttt acagcattac cgtctggcgc gtgatgtgga aattgtcgtt gttgatggtg
     3361 tgcgtcgctt tggcaatggc tggtggttgc cggcggggcc aatgcgtgag cgagcggggc
     3421 gcttaaagtc agttgatgcg gtaatcgtca acggcggtgt cccccgcagc ggtgaaatcc
     3481 ccatgcatct gctgccgggt caggcggtga atttacgtac cggtacgcgt tgtgacgttg
     3541 ctcagcttga acatgtggtg gcgatggcag ggattgggca tccgccgcgc ttttttgcca
     3601 cgctgaagat gtgcggcgta caaccggaaa aatgtgtacc gctggccgat catcagtctt
     3661 tgaaccatgc ggatgtcagc gcgttggtaa gcaccgggca aacgctggta atgactgaaa
     3721 aagatgcggt gaaatgccgg gcctttgcag aagaaaattg gtggtatttg cccgttgacg
     3781 cacagctttc aggtgatgaa ccagcgaaac tgcttgcgca actaacctcg ctggcttctg
     3841 gcaactagtt acgccgcggc agcgttcgat tgatggagtc atgaatgtcg ctgccgcacc
     3901 tctcccttgc tgatgcgcgt aatcttcacc ttgccgcgca aggcctgtta aacaaacccc
     3961 gccgtcgagc gtcgttggag gatattccgg caacgatctc ccgcatgtcc ttgctgcaaa
     4021 tcgataccat caatattgtt gcccgtagcc catatctggt gcttttcagt cgtctgggaa
     4081 attatcctgc ccagtggctg gatgagtctc tggcgcgtgg cgaattaatg gaatactggg
     4141 cgcatgaagc ctgctttatg ccacgcagcg actttcgtct tattcgccac cgcatgctgg
     4201 cacctgaaaa aatgggctgg aaatacaaag acgcctggat gcaggaacat gaggcggaaa
     4261 ttgcacagtt aattcagcat attcatgata aggggccggt acgttcagcc gattttgagc
     4321 atcctcgtaa aggtgcaagc ggctggtggg aatggaagcc gcataaacgg catctggaag
     4381 gtttatttac tgccggaaag gtgatggtga ttgaacggcg caacttccag cgcgtttatg
     4441 atttaaccca ccgtgtcatg cctgactggg atgatgagcg cgatctcgtt tcgcaaacag
     4501 aagcagaaat catcatgctg gataacagtg cgcgtagcct gggaatattc cgcgaacagt
     4561 ggctggcaga ttactatcgg ctgaaacgtc cggcactggc agcgtggcgc gaagcgaggg
     4621 ctgaacagcg gcaaatcatt gctgtgcatg ttgaaaaatt gggcaatctt tggctgcatg
     4681 ctgatttgct gccgctactc gagcgagcgc tggccggaaa gctcactgca acgcacagcg
     4741 cggtactttc gccttttgat cctgttgtct gggatcgcaa acgcgcagag cagctttttg
     4801 attttagcta ccggctggag tgctataccc cagcgccgaa acgccagtat ggctattttg
     4861 ttctgccgtt attacatcgt gggcaattag ttgggcgaat ggatgccaaa atgcatcgcc
     4921 agacaggcat ccttgaagtt atctctctgt ggttacagga aggtattaaa ccaacgacaa
     4981 cgctgcaaaa agggttacgt caggcgatta ctgatttcgc taactggcag caggcaacgc
     5041 gggtgacatt aggacactgc ccgcaaggtc tctttacgga ttgccgcacc ggctgggaaa
     5101 tagaccccgt cgcataaatg attatgataa gatttatgca ttcataaccc gatccatctg
     5161 gaggaactat ggatcatcgt ctgcttgaaa tcattgcctg cccggtttgc aacggaaaac
     5221 tttggtataa ccaggaaaaa caagagctta tttgcaaact ggacaacctg gctttcccgc
     5281 tgcgcgatgg cattccagtg ttgctggaaa cggaagcccg cgtgctgact gctgatgaga
     5341 gtaaatcatg agttttgtgg tcattattcc cgcgcgctac gcgtcaacgc gtctgcccgg
     5401 taaaccattg gttgatatta atggcaaacc catgattgtt catgttcttg aacgtgctcg
     5461 cgaatcaggt gccgagcgca tcatcgtggc aaccgatcat gaggatgtcg cccgcgccgt
     5521 tgaagccgct ggcggtgaag tatgtatgac gcgcgccgat caccagtcag ggacagaacg
     5581 cttggcggaa gttgtcgaaa aatgcgcgtt cagcgacgac acggtgatcg ttaatgtgca
     5641 gggtgatgaa ccgatgatcc ctgcgacaat cattcgtcag gttgctgata acctcgctca
     5701 gcgtcaggtg ggtatggcga ctctggcggt gccaatccac aatgcggaag aagcgtttaa
     5761 cccgaatgcg gtgaaagtgg ttctcgacgc tgaagggtat gcactgtact tctcacgcgc
     5821 taccattccg tgggatcgtg atcgttttgc aaaaggcctt gaaaccgttg gcgataactt
     5881 cctgcgtcat cttggtattt atggctaccg tgcaggcttt atccgtcgtt acgtcacctg
     5941 gcagccaagc ccgttagaac acatcgaaat gttagagcag cttcgtgttc tgtggtacgg
     6001 cgaaaaaatc cacgttgctg tcgcgcatga agttcctggc acaggtgtgg atacccctga
     6061 agatctcaag cgcgttcgcg ctgaaatgcg ctaatttcac ttcacgacac ttcagccaat
     6121 tttgggagga gtgtcgtacc gttacgattt tcctcaattt ttcttttcaa caattgatct
     6181 cattcaggtg acatctttta tattggcgct cattatgaaa gcagtagctt ttatgagggt
     6241 aatctgaatg gaacagctgc gtgccgaatt aagccattta ctgggcgaaa aactcagtcg
     6301 tattgagtgc gtcaatgaaa aagcggatac ggcgttgtgg gctttgtatg acagccaggg
     6361 aaacccaatg ccgttaatgg caagaagctt tagtacgccc ggaaaagccc gacaactggc
     6421 atggaaaacc accatgctgg caagaagtgg gactgtccgt atgccgacta tttatggtgt
     6481 gatgacgcat gaagaacacc ccggccctga tgtcctgcta ctggagcgga tgcgtggtgt
     6541 ttcggtggag gcaccagccc gaacaccaga acgttgggaa cagctcaaag accaaatcgt
     6601 tgaagcctta ctggcctggc accgtcagga cagtcgcggt tgcgtcggcg cggtcgacaa
     6661 tactcaggaa aatttctggc cctcatggta ccggcaacat gttgaagtgc tatggaccac
     6721 gctcaatcag ttcaataaca ccggtctgac gatgcaggat aagcggatcc tgtttcgcac
     6781 tcgcgaatgt ctcccggcat tatttgaagg ctttaacgac aattgtgtgt tgattcacgg
     6841 taacttctgt ttacgcagca tgctgaaaga ttcgcgcagc gatcagttac tggcgatggt
     6901 cgggccggga ctaatgcttt gggcaccgcg agaatacgaa ctgttccgac taatggataa
     6961 ttctctggcg gaagatttgc tctggagtta cctgcaacgc gcgccagtgg cggagtcgtt
     7021 catctggcgg cgttggttgt atgtgttatg ggatgaagtt gcgcaactgg ttaataccgg
     7081 acgatttagt cggcgcaact tcgatctggc atcaaaatca ctcttgccgt ggctcgcctg
     7141 acggaccttt cagccactgc cagatgcggc caagcgtttc atagccaaca cgatcgctat
     7201 gcatcagcca gaccggagaa gggattgccc gttcccacgg attaagcggc gagtcgatgg
     7261 ccaactgatt cgccggggct ggtagcggat ttaacccttc ctgctgaaaa aagatcatcg
     7321 cccgcggcag atgtgatgcg gaggtcacca gcaggaaagg ggcgtcacca atcgcctgtt
     7381 tcactgccgc agcttcttct tcggtatctt ttggcaaatc cagggtgata atttgctcgc
     7441 gcggtacgcc cagcgattgc gcaactctgg cacctacttc cgctgtactc accgtattgg
     7501 tttttgctac gcctcccgtg aagatcagtt ttgaacccgg attatcgcgc cataaacgga
     7561 taccctcatt caggcgtggc agactgttat tgattaaatt agagctcggt gcccactgcg
     7621 ggttccaggt ataaccacct ccgagcacca cgatatagtc cactttttgc gaattattcc
     7681 atgtcggata ggtgctttcg atgggacgca gcaggcggtc ggctaccggt tgcaaactca
     7741 ataacaagag tgacagccac ccaatgctaa taaaaatctt cccggttttt tgaaaacggc
     7801 taaaccacag cagcgccagg ccagcgccaa taattaacaa catcagcgga agtggcagca
     7861 acatattgcc aatcactttt ttcagtgtaa aaagcatcct ttctggttcc ttttttaacc
     7921 atatagcaag ggatcgccag cgatattaca ccagacgggt tcattctcgc cgtggctgtg
     7981 acaaaatagc gtctttatca gaaaacgtct tagccaaccg gtggagaaag caatgcagga
     8041 tcgcaatttt gatgatattg cggaaaagtt ttcccgtaac atttacggca ccaccaaagg
     8101 gcagcttcga caggctattc tgtggcagga tctcgatcgc gtgctggcgg aaatgggtcc
     8161 gcaaaaactg cgtgtgctgg atgcgggcgg tggagaaggg cagaccgcaa tcaaaatggc
     8221 cgagcgcggg catcaggtca ttttatgcga tctttctgcg cagatgatcg accgcgcaaa
     8281 acaggcggca gaagcaaaag gtgtgagcga caacatgcaa tttatacatt gcgccgctca
     8341 ggatgttgct tcgcatttgg aaacgcccgt tgatctgata ttgttccatg cggtgctcga
     8401 gtgggtggct gatccccgca gcgtattgca gaccctgtgg tcagtgttgc gtccaggcgg
     8461 cgtgttgtcg ttaatgttct acaatgcgca tggtttgttg atgcataaca tggtcgccgg
     8521 gaattttgat tacgtgcagg cgggaatgcc gaaaaagaaa aaacggacgc tttcgccaga
     8581 ttatccacgc gacccggcgc aggtttatct gtggctggaa gaagctggtt ggcaaattat
     8641 gggtaagaca ggcgttcgcg tgtttcatga ttatctgcgc gagaagcacc agcagcgcga
     8701 ctgctatgaa gcattacttg aattagaaac gcgttattgc cgtcaggaac cgtatattac
     8761 cctggggcgt tatattcatg tcaccgcgcg caaaccgcag agcaaggata aagtatgagt
     8821 gaattttccc agacagtccc cgaactggtt gcctgggcca gaaaaaatga cttctccatc
     8881 tcgctgccgg tagaccgact ctcttttctg ctggcggttg ccacgctgaa cggcgagcgt
     8941 ctggatggtg agatgagtga aggcgagctg gtggatgcat tccgccatgt gagtgatgcg
     9001 tttgagcaaa ccagcgaaac catcggcgtg cgcgccaata acgcgatcaa cgacatggtg
     9061 cgtcaacgtc tgctgaaccg ctttaccagc gagcaggcag aagggaacgc aatttaccgt
     9121 ctgacgccgc tcggcatcgg cattactgac tattacatcc gtcagcgcga gttttctacg
     9181 ctgcgccttt ctatgcagtt gtcgattgtg gcgggtgagc tcaaacgcgc agcagatgcc
     9241 gccgaagagg gcggtgatga atttcactgg caccgtaatg tctatgcgcc actgaaatat
     9301 tcggtagcag aaattttcga cagtatcgac ctgacgcaac gtctgatgga cgaacagcag
     9361 cagcaggtga aggacgatat cgcccagttg ctgaacaaag actggcgggc ggcgatttcc
     9421 agctgtgaat tgttgctttc ggaaacttcc ggaacgctgc gtgaattgca ggatacactg
     9481 gaagcggcag gcgacaaatt gcaggctaat ctgttgcgca ttcaggatgc gacgatgact
     9541 catgacgatc tgcattttgt cgatcgtctg gtgttcgatc tgcagagcaa actcgaccgt
     9601 attatcagtt ggggccagca atccatcgac ttgtggattg gctacgaccg ccacgtacac
     9661 aaatttattc gtaccgcgat cgatatggat aaaaaccgcg tctttgctca gcggttacgt
     9721 cagtcggtac aaacctattt tgatgagccg tgggcgctaa cttatgccaa tgccgatcgt
     9781 ctgctggata tgcgtgacga agagatggca ctgcgcgatg aagaagtgac tggggaactt
     9841 cctgaggatc tggaatacga agagtttaac gagatccgcg aacagctggc ggcgatcatc
     9901 gaagaacaac ttgctgtgta caaaaccaga caagtgccgc tggatcttgg tctggtggta
     9961 cgcgaatatc tgtcacagta tccgcgtgca cgtcactttg acgttgcgcg cattgttatt
    10021 gatcaggcgg tacgtcttgg cgtagcgcaa gcagatttca ccggactgcc agcgaaatgg
    10081 cagccgatta atgattacgg agccaaggta caggcgcatg tcatcgacaa atattgaaca
    10141 agtgatgccg gttaagctgg cgcaggcgct ggcgaatccg ttatttccgg cgctggacag
    10201 cgccttacgt tcaggccgcc atattggcct cgacgaactg gataatcatg cattcctgat
    10261 ggattttcag gaatatctgg aagagtttta cgcgcgttat aacgttgagc ttattcgtgc
    10321 accggaaggg ttcttctatt tacgtccacg ttccaccacg ctgatccctc gttccgtctt
    10381 gtcggaactg gatatgatgg tcgggaaaat cctctgctat ctctatctca gcccggaacg
    10441 gctggcgaat gaggggattt ttactcaaca ggaactgtac gacgaactgc tcaccctggc
    10501 cgatgaagcg aaactgctga aactggtgaa caaccgttca accggttcag acgttgaccg
    10561 tcagaagttg caggaaaaag tacgttcttc gctcaaccgt ctgcgtcgtt taggcatggt
    10621 gtggtttatg ggccacgaca gcagcaagtt ccgcattacc gaatcggtgt tccgcttcgg
    10681 agccgatgtg cgtgctggcg acgatccccg tgaagcacag cgtcgcctga ttcgtgatgg
    10741 cgaagcaatg ccgattgaaa atcatctgca actcaacgat gaaaccgaag agaatcagcc
    10801 agatagcgga gaggaagaat aatgattgaa cgcggtaaat ttcgctcact gacgctgatt
    10861 aactggaacg gcttttttgc ccgaactttt gaccttgacg agctggtcac gacgctttct
    10921 ggcggtaacg gggcgggtaa atccaccacc atggcggcgt tcgttacggc gctgatcccc
    10981 gacctgaccc tgctgcattt ccgtaacact acggaagccg gggccaccag cggttcgcgc
    11041 gataaaggtc tgcacggtaa gctgaaagcg ggtgtctgtt attcgatgct cgacaccatt
    11101 aactcgcgcc accagcgcgt ggtggtcggt gtgcgtctgc aacaggttgc cggacgcgat
    11161 cgtaaagtgg atatcaaacc atttgccatt cagggactgc cgatgtcggt gcagccgaca
    11221 cagctggtga ccgaaactct gaacgaacgc caggcgcgcg tgctgccgct taacgagctg
    11281 aaagacaagc tcgaggcgat ggaaggcgtg cagtttaaac agttcaactc cattaccgat
    11341 taccactcgc tgatgttcga tctgggcatc atcgcgcgtc gtctgcgctc cgcatctgac
    11401 cgtagcaaat tctatcgtct gatcgaagct tcgctgtatg gcgggatctc cagtgccatt
    11461 acccgttctc tgcgcgacta cctgttgccg gaaaacagcg gcgtgcgtaa agcgttccag
    11521 gacatggaag cggcgctgcg tgaaaaccgc atgacgctgg aagcgattcg tgtcacccaa
    11581 tcggaccgtg acctgtttaa gcatctgatc agcgaagcta ccaactacgt ggcggcggac
    11641 tacatgcgtc acgccaacga gcgccgtgtc catctcgaca aagcgctgga gtttcgtcgc
    11701 gagctacata cttcgcgtca gcaactggcg gctgagcagt acaaacacgt cgatatggcg
    11761 cgtgagctgg cagagcacaa cggtgccgaa ggcgatctgg aagcggatta tcaggcggcc
    11821 agcgatcacc tgaacctggt gcaaaccgca ctgcgtcagc aggagaagat cgaacgctac
    11881 gaagcggatc tcgatgagct gcagatccgt ctggaagagc aaaacgaagt ggtggcagaa
    11941 gccatcgaac gccaggaaga gaacgaggct cgtgcggaag ctgccgaact ggaagtggac
    12001 gagctgaaaa gccagcttgc tgactaccag caggcgctgg acgtccagca aacgcgcgcg
    12061 atccagtata accaggcgat tgctgcgctt aatcgtgcca aagaactgtg ccatctgccg
    12121 gacttaaccg ccgacagcgc cgccgaatgg ctggaaacct tccaggcgaa agagctggaa
    12181 gcgactgaaa aaatgctctc tcttgagcag aaaatgagca tggcgcaaac cgcgcacagc
    12241 cagtttgagc aggcttatca gctggtggtg gcaatcaacg gcccgctggc gcgtaacgag
    12301 gcgtgggatg tcgcccgcga actgctgcgc gaaggggtcg agcagcgtca cctggcagat
    12361 caggttcaac cgttgcggat gcgcttaagt gaactggaac agcgtctgcg cgagcagcaa
    12421 gaagccgaac gcctgctggc agatttctgc aaacgtcagg gcaagaattt tgatatcgac
    12481 gaactggaag ccctgcatca ggagctggaa gcgcgtattg cctctctttc cgatagcgtg
    12541 tctaacgccc gcgaagagcg tatggcactg cgccaggagc aggaacagct gcagtctcgc
    12601 attcagagtt tgatgcagcg tgcaccggtt tggctggcag cgcaaaacag tctcaaccag
    12661 ttgagtgaac agtgcggcga agagttctcc tccagccagg acgtcactga atatctgcaa
    12721 cagttgctgg agcgtgagcg cgaggcgatt gttgaacgcg atgaagtagg cgcgcgcaaa
    12781 aacgccgtcg atgaagagat cgaacgttta agccagcctg gcggctctga agatcagcgt
    12841 ctgaacgcgc tggcggagcg ttttggtggt gtgctgctgt cagaaattta tgatgacgtt
    12901 agcctggaag atgcgccgta cttctcagcg ctgtatggcc cgtcacgcca cgccatcgtg
    12961 gtgccagatc tatcacaggt aactgaacac ctggagggct tgaccgattg cccggaagat
    13021 ctctatttga tcgaagggga tccgcagtca ttcgatgaca gcgtgttcag cgttgatgag
    13081 ctggaaaaag cggtagtggt gaaaatcgcc gatcgtcagt ggcgttattc acgtttcccg
    13141 gaagtgccgc tgtttggtcg tgctgcgcgt gaaagccgta ttgaaagcct ccatgccgag
    13201 cgtgaagtgc tttccgaacg cttcgccacg ctctcctttg acgtacagaa aacccagcgt
    13261 ctgcatcagg cgttcagccg ctttatcggt agtcatctgg cggtggcgtt tgagtctgac
    13321 ccggaagcag aaatccgtca actgaacagc cgtcgcgtcg aactggagcg ggcgttgagt
    13381 aatcatgaaa atgataacca gcagcagcgt attcagtttg agcaggcgaa agaaggcgtt
    13441 acggcgctga accgcattct gccgcgtctc aacctgttgg ctgatgacag cctggcggat
    13501 cgcgtcgatg aaatccgcga acgtctggat gaagcccagg aagccgcgcg ttttgttcag
    13561 cagtttggca atcaactggc gaaactggaa ccgatcgttt cggtattgca gagcgacccg
    13621 gaacagttcg aacagttaaa agaagattac gcgtactctc agcagatgca gcgcgatgcc
    13681 cgtcagcagg cgtttgccct gacggaagtg gtgcagcgtc gtgcgcactt tagctattct
    13741 gactcggcag aaatgcttag cggtaacaac gatctcaacg aaaaactgcg tgaacgtctg
    13801 gaacaggcgg aagcggagcg tacccgcgct cgcgaagcgt tgcgcggtca cgcagcgcag
    13861 ttgagtcagt acaaccaggt gctggcttcg ctgaaaagtt cttacgacac caaaaaagag
    13921 ctactcaacg atctgcaacg tgaattgcag gatatcggcg tgcgtgctga tagcggggca
    13981 gaagagcggg cacgtattcg ccgtgacgag ctgcatgcgc aactgagcaa taaccgttcg
    14041 cgccgcaacc aactggaaaa agcgctcacc ttctgcgaag ctgagatgga caacctgacc
    14101 cgcaaactgc gcaagctgga gcgtgattac tttgagatgc gcgagcaggt agtgaccgcg
    14161 aaagcgggct ggtgtgcagt catgcgcatg gtgaaagata acggcgttga gcgccgctta
    14221 caccgtcgtg agctggctta tctctccgct gatgatttgc gttccatgtc ggataaggcg
    14281 ttaggtgcgc tgcgtctggc ggtggcggat aacgaacatc tgcgcgacgt gctgcgcatg
    14341 tcggaagatc cgaaacgtcc ggagcgtaaa attcagttct tcgtggcggt ttatcagcat
    14401 ctgcgtgaac gtattcgtca ggatattatt cgtaccgatg atccggtgga agctatcgaa
    14461 cagatggaga tcgaacttag ccgtctgacc gaagagttaa cctcccgtga acagaaactg
    14521 gcgatcagtt cccgcagcgt ggcgaacatc attcgcaaaa ccattcagcg cgaacagaat
    14581 cgtatccgta tgctcaacca ggggttgcag aacgtatcgt ttggtcaggt gaacagcgta
    14641 cgtctgaacg tgaacgtgcg tgaaacgcac gccatgctgt tggatgtgct ctctgaacag
    14701 cacgagcagc atcaggatct gtttaacagc aaccgtctga ccttctcgga agcgctggcg
    14761 aaattgtatc agcgtcttaa tccgcagatt gatatggggc agcgcacgcc gcagaccatt
    14821 ggcgaagaac tgctggatta ccgcaactat ctggaaatgg aagttgaggt taaccgtggt
    14881 tccgatggct ggttgcgcgc agagtctggt gcattgtcga ccggtgaggc gattggtacc
    14941 ggtatgtcga ttctggtgat ggtggtacaa agctgggaag atgaatctcg ccgcctgcgc
    15001 ggtaaagata tctctccttg ccgcctgctg ttcctcgatg aagcagcgcg actggatgct
    15061 cgttctatcg ccacgctgtt tgaattgtgt gaacgtttgc aaatgcagct catcatcgca
    15121 gcgccggaaa atatcagccc ggagaaaggc accacctata aactggtgcg taaagtcttc
    15181 cagaataccg aacacgttca cgtcgtcggc ctgcgaggat ttgcgccgca actccctgaa
    15241 acgcttccag gaagtgacga agcgccttct caggcgagtt aaaattaagg cggcagcaat
    15301 gccgcctttt ctttttccga aaactccgtt tctgcactaa aaaagtggca cattacggcg
    15361 cgcttttctt taaacttctt tacattaggt tatgtaaaaa cgtatcggcg tttatatact
    15421 gaagataagc ctgatgagta acaggcttgc tcgtcatact ttcgtgagta ttggcgttgt
    15481 acaggcaagt cgtaaaataa cagcctggct attcagagta tgataaaaac agggggcaag
    15541 ggatgttgct taatatgatg tgtggtcgtc ggctgtcggc aatcagtttg tgcctggccg
    15601 taacattcgc tccactgttc aatgcgcagg ccgatgagcc tgaagtaatc cctggcgaca
    15661 gcccggtggc tgtcagtgaa cagggcgagg cactgccgca ggcgcaagcc acggcaataa
    15721 tggcggggat cctgccattg cctgaaggtg cggcagaaaa agcccgcacg caaatcgaat
    15781 ctcaattacc cgcaggttac aagccggttt atcttaacca gcttcaactg ttgtatgccg
    15841 cacgcgatat gcaacccatg tgggaaaacc gtgatgctgt taaagccttc cagcaacagc
    15901 tggcagaggt ggcgattgcc ggtttccagc cgcagtttaa taaatgggta gagttactga
    15961 ccgatcctgg tgttaacggg atggcacgcg acgtggtgct ctctgatgcg atgatgggct
    16021 atctccattt cattgcaaat attccggtca aaggcactcg ctggctatat agcagtaaac
    16081 cttatgcgct ttcaacgccg ccgctttcgg tgattaacca atggcagcag gcgctggata
    16141 aaggtcaatt gcctacgttt gttgcaggac tggcaccgca gcatccgcaa tatgcggcga
    16201 tgcatgaatc gttactggcc ttactcagtg acaccaaacc gtggccccaa ctgaccggca
    16261 aagcaacgtt gcgcccaggg cagtggagta acgacgtacc ggcgttgcgc gaaatattgc
    16321 aacgcacagg catgttggac ggggggccga aaattactct acctggcgat gacacgccaa
    16381 ctgacgcggt agtcagccca tccgctgtta ctgttgaaac agcagaaact aagccgatgg
    16441 acaagcaaac gacgtctcgt agtaaacctg cgcctgccgt tcgtgccgcc tacgataatg
    16501 aactggtgga agccgttaaa cgttttcagg catggcaagg attgggggca gatggtgcta
    16561 ttggcccggc aacgcgggac tggttaaacg taacgcccgc ccagcgtgct ggcgtgttgg
    16621 cactcaacat ccagcgattg cgcttgctgc caacagagct ttctaccggg atcatggtta
    16681 acattccggc ctattcgctg gtctactatc agaacggcaa tcaggtgctg gattcgcgag
    16741 tcattgtcgg tcgccccgat cgcaaaacgc cgatgatgag cagtgcactt aacaacgtag
    16801 tggtaaaccc gccgtggaac gtaccaccaa ctctggcacg caaagatatt ctgccgaaag
    16861 tgcgcaacga tccgggatat ctcgaaagcc atggctatac ggtgatgcgc ggctggaaca
    16921 gcagagaagc gattgaccca tggcaggttg actggtctac aatcacggcc tcgaatttac
    16981 cgtttcgctt ccagcaggct ccaggcccac ggaactcgct ggggcgctat aaattcaata
    17041 tgccgagttc agaggccatt tatttgcatg acacgccgaa ccacaatctg ttcaagcgtg
    17101 atacacgcgc attgagctca ggctgtgtac gagtgaataa agcttccgat ctggcgaata
    17161 tgctgttgca ggatgcaggc tggaatgaca aacgtatttc tgatgcgctg aagcagggtg
    17221 atacacgtta cgtcaatatt cggcagtcga ttccggtgaa tctctactac ctgacggcct
    17281 ttgttggtgc agatggtcgt acccagtatc gtacagatat ttacaattat gatctgcctg
    17341 cgcgatccag ctcgcaaatc gtatcgaaag cggaacaatt aatcaggtaa atgaagtagt
    17401 tcggggaaat cagttgtcgt aataattagc atgattgggg gtggttctct gcagcccccg
    17461 tcactgctgg ggttgagtca tcttgacgtc tgctttacgg gcggttaagg tgcctcttgt
    17521 gcgccagaag tgcatataaa cgataacatt gacctgtaga cttgattatc atggacaaat
    17581 tcgacgctaa tcgccgcaaa ttgctggcgc ttggtggcgt tgcactcggt gccgccatcc
    17641 tgccgacccc tgcgtttgca acactctcta ccccacgccc gcgcattttg acactcaata
    17701 atcttcatac cggagagtca atcaaagcgg agtttttcga tggcagaggc tatattcagg
    17761 aagaattggc aaaacttaac cattttttcc gcgattaccg cgcgaacaaa ataaagtcca
    17821 tcgacccagg attattcgac cagttgtatc gcctgcaagg gttattaggc acccgcaaac
    17881 cggtgcaact catttccggt tatcgttcca ttgataccaa caatgaacta cgcgcccgca
    17941 gccgtggagt agcgaagaaa agctatcaca ctaaaggcca ggcgatggat ttccatattg
    18001 aaggtatcgc gttaagcaat attcgcaaag ccgcgttatc tatgcgcgca ggtggtgtag
    18061 gatattaccc acgtagtaac tttgtgcata ttgataccgg gccagcacgg cactggtagt
    18121 aattgcttaa cgaaacaggg gcagtatgaa ctatcgtatt attccggtca ccgcattctc
    18181 ccagaactgt tcattgatct ggtgtgaaca aacccgactg gccgcactgg tcgatcctgg
    18241 cggcgatgcg gaaaaaatca aacaggaagt tgatgccagc ggcctgacac tgatgcagat
    18301 cctgctgacg catggtcatc tggaccacgt tggcgcagcg gcggaactgg cgcaacatta
    18361 cggcgtgccg gtttttggcc cggaaaaaga agatgagttc tggctgcaag gcttgcctgc
    18421 gcaaagtcgt atgtttggtc tggaagagtg tcagccgctg acgccagatc gttggctgaa
    18481 agaaggcgat accatcagca tagggaatgt gactttacag gtgttacatt gccctgggca
    18541 tacgccgggt catgtcgtgt tttttgatga tcgggcaaag ctgctgattt ctggcgatgt
    18601 tattttcaaa ggcggagtag ggcgcagtga cttcccgcgt ggcgatcata atcaactgat
    18661 ttcttcaatc aaagataaat tgctgccact gggggatgac gtgacattta ttccgggtca
    18721 cggaccatta tccacacttg gttatgaacg cctgcataat cccttcctgc aagacgaaat
    18781 gcccgtctgg taaggcacac ataaaaaagc ccgcttttca gcgggcttca ttgtttttaa
    18841 cgcttacagc actgccacaa tcgcttcgca cagcggagcc atgttatctg gtgtcatccc
    18901 tgccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcaa
    18961 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
    19021 gaagtcgcgg tttgcccctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
    19081 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
    19141 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
    19201 cgccgctttc atttggctga atgcgcgatc aacggtttca ctgtcggcag caaccagagt
    19261 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
    19321 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
    19381 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
    19441 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
    19501 atggaacagc actacgtcgc cagcctgagc ttcgttcagg ctgttaatca gtgcatcgaa
    19561 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
    19621 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
    19681 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
    19741 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
    19801 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
    19861 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
    19921 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
    19981 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
    20041 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
    20101 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
    20161 cataaaaaaa acaggaccaa agtcctgttt tttcggcatt taacaaagag gtgtgctatt
    20221 agaactggta aacgatgccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
    20281 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
    20341 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
    20401 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
    20461 caactaacag aacgtcttgc gttttgttgg cgaagccgcc ttcaattggc gtagcgttac
    20521 gggtttcacc gtagttcgct gccaggtaga tgttgttcgc gtcatacttc agaccagttg
    20581 cccactgttc agctttttta ccctgaccgc ggaattcggc ttcctgggcg tcggtacggt
    20641 ctgctgcacc gtaagcacct acgataccaa agccttcgaa ttcgtagctg atagagccac
    20701 cgacgccgtc gccgttggaa cgctcaggaa taccagcacg ttcgtttttg cccaggtact
    20761 gaacggcaaa gttcaggccg tcaaccagac cgaagaagtt ggagttacga taggtagcaa
    20821 cgccgcctac acgaccaacg aagaagtcat cgctgtatgc cgtgtcaccg ccaaactcag
    20881 gcagcatatc ggtgtaaccc agtgcgtcgt aaaccacacc gtagttacga ccgtagtcga
    20941 tagaacccgc atcagcgaat ttcagacctg cgaatgccaa acgagttttg ttacctttct
    21001 gggcatcagc gccttcagag ttgttaccct ggaagttata ttcccactga ccataaccgg
    21061 tcagatcgga attgatttgt gtttcccctt taaaaccaag acgggcataa gttttgtcgc
    21121 cgtttccgcc gtaactgttt acaccattgt ctttagaaaa ataatgcaga ccgacagctt
    21181 taccgtacag atctactttg ttgccatctt tgttatagat ttctgcagcg tttgcagtac
    21241 ctgctactaa cagagcaggg acgatcactg ccagaatatt gcgcttcatc attatttatt
    21301 accctcatgg tttttttatg acacctgcca ctgccgtcaa taagttctgt caataaaaat
    21361 ttacggaact attgatgaga gtttggtgtc tttatgtgtc tgcaggcatc tttccattca
    21421 agct
//
LOCUS       DAZKFU020000065        21089 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZKFU020000065 DAZKFU020000000
VERSION     DAZKFU020000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.065"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..575
                     /gene="lpfA1"
                     /locus_tag="ACHOQQ_RS09520"
                     /old_locus_tag="pgaptmp_001904"
     CDS             51..575
                     /gene="lpfA1"
                     /locus_tag="ACHOQQ_RS09520"
                     /old_locus_tag="pgaptmp_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HGU4470163.1"
                     /translation="MKKVVFALTALALTSGSAFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADQSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            640..1326
                     /gene="lpfB"
                     /locus_tag="ACHOQQ_RS09525"
                     /old_locus_tag="pgaptmp_001905"
     CDS             640..1326
                     /gene="lpfB"
                     /locus_tag="ACHOQQ_RS09525"
                     /old_locus_tag="pgaptmp_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HGU4470164.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSATRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFRLDPGKNNILRIVNTTPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLQGDVKTAPNELRFTRSGNQI
                     RVDNPTVFNITFNQFFANEKEIEKAGMVPAKGSLNITLPAGAGSVSKIKYNAINDFGS
                     SAEMMTKNVD"
     gene            1355..3928
                     /gene="lpfC"
                     /locus_tag="ACHOQQ_RS09530"
                     /old_locus_tag="pgaptmp_001906"
     CDS             1355..3928
                     /gene="lpfC"
                     /locus_tag="ACHOQQ_RS09530"
                     /old_locus_tag="pgaptmp_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HGU4470165.1"
                     /translation="MSRKTVSRTFSSFSISVVAVAVASTFSAHAGKFNPKFLEDVQGV
                     GQHVDLTMFEKGQEQQLPGIYRVSVYVNEQRMETRTLEFKEATEAQRKAMGESLVPCL
                     SRTQLAEMGVRVESFPALNLVPAEACVPFDEIIPQASSHFDFSEQKLVLSFPQAAMHQ
                     VARGTVPESLWDEGIPALLLDYSFSGSNSEYDSSGSSSSYVDDNGTVHHDDGNDTLKS
                     DSYYLNLRSGLNLGAWRLRNYSTWSHSGGKAQWDNIGTSLSRAIIPFKAQLTMGDTAT
                     AGDIFDSVQMRGAMLASDEEMLPDSQRGFAPIVRGIAKSNAEVSIEQNGYVIYRTYVQ
                     PGAFEINDLYPTANSGDLTVIIKEADGSEQRFIQPFSSVPIFQREGHLKYSFAAGEYQ
                     AGNYDSASPRFGQLDLIYGLPWGMTAYGGVLISNNYNAFALGIGKNFGYIGAISIDVT
                     QAKSELNNDRDSQGQSYRFLYSKSFESGTDFRLAGYRYSTSGFYTFQEATDVRSDADS
                     DYNRYHKRSEIQGNLTQQLGAYGSVYLNLTQQDYWNDAGKQNTVSAGYNGRIGKVSYS
                     IAYSWNKSPEWDESDRLWSFNISVPLGRAWSNYRVTTDQDGRTNQQVGVSGTLLEDRN
                     LSYSVQEGYASNGVGNSGNANVGYQGGSGNVNVGYSYGKDYRQLNYSVRGGVIVHSEG
                     VTLSQPLGETMTLISVPGARNARVVNNGGVQVDWMGNAIVPYAMPYRENEISLRSDSL
                     GDDVDVENAFQKVVPTRGAIVRARFDTRVGYRVLMTLLRSAGSPVPFGATATLITDKQ
                     NEVSSIVGEEGQLYISGMPEEGRVLIKWGNDASQQCVAPYKLSLELKQGGIIPVSANC
                     Q"
     gene            3943..4998
                     /locus_tag="ACHOQQ_RS09535"
                     /old_locus_tag="pgaptmp_001907"
     CDS             3943..4998
                     /locus_tag="ACHOQQ_RS09535"
                     /old_locus_tag="pgaptmp_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4470166.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRSV
                     DSMKYYTLTEELEVATKIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTITQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLGFDCNNISDGIKVSLRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDASSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            5003..5533
                     /gene="lpfE"
                     /locus_tag="ACHOQQ_RS09540"
                     /old_locus_tag="pgaptmp_001908"
     CDS             5003..5533
                     /gene="lpfE"
                     /locus_tag="ACHOQQ_RS09540"
                     /old_locus_tag="pgaptmp_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HGU4470167.1"
                     /translation="MKFNLLLHSGIASLSLFACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5788..7479
                     /gene="eptB"
                     /locus_tag="ACHOQQ_RS09545"
                     /old_locus_tag="pgaptmp_001909"
     CDS             5788..7479
                     /gene="eptB"
                     /locus_tag="ACHOQQ_RS09545"
                     /old_locus_tag="pgaptmp_001909"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGU4470168.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAVAN"
     gene            7571..7647
                     /locus_tag="ACHOQQ_RS09550"
                     /old_locus_tag="pgaptmp_001910"
     tRNA            7571..7647
                     /locus_tag="ACHOQQ_RS09550"
                     /old_locus_tag="pgaptmp_001910"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7605..7607,aa:Pro,seq:cgg)
     gene            8392..9999
                     /gene="dppA"
                     /locus_tag="ACHOQQ_RS09555"
                     /old_locus_tag="pgaptmp_001911"
     CDS             8392..9999
                     /gene="dppA"
                     /locus_tag="ACHOQQ_RS09555"
                     /old_locus_tag="pgaptmp_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGU4470169.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10307..11326
                     /gene="dppB"
                     /locus_tag="ACHOQQ_RS09560"
                     /old_locus_tag="pgaptmp_001912"
     CDS             10307..11326
                     /gene="dppB"
                     /locus_tag="ACHOQQ_RS09560"
                     /old_locus_tag="pgaptmp_001912"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGU4470170.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11336..12238
                     /gene="dppC"
                     /locus_tag="ACHOQQ_RS09565"
                     /old_locus_tag="pgaptmp_001913"
     CDS             11336..12238
                     /gene="dppC"
                     /locus_tag="ACHOQQ_RS09565"
                     /old_locus_tag="pgaptmp_001913"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGU4470171.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12249..13232
                     /gene="dppD"
                     /locus_tag="ACHOQQ_RS09570"
                     /old_locus_tag="pgaptmp_001914"
     CDS             12249..13232
                     /gene="dppD"
                     /locus_tag="ACHOQQ_RS09570"
                     /old_locus_tag="pgaptmp_001914"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGU4470172.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13229..14233
                     /gene="dppF"
                     /locus_tag="ACHOQQ_RS09575"
                     /old_locus_tag="pgaptmp_001915"
     CDS             13229..14233
                     /gene="dppF"
                     /locus_tag="ACHOQQ_RS09575"
                     /old_locus_tag="pgaptmp_001915"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGU4470173.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14263..15534)
                     /locus_tag="ACHOQQ_RS09580"
                     /old_locus_tag="pgaptmp_001916"
     CDS             complement(14263..15534)
                     /locus_tag="ACHOQQ_RS09580"
                     /old_locus_tag="pgaptmp_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGU4470174.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16010..16117
                     /locus_tag="ACHOQQ_RS09585"
                     /old_locus_tag="pgaptmp_001917"
     CDS             16010..16117
                     /locus_tag="ACHOQQ_RS09585"
                     /old_locus_tag="pgaptmp_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4470175.1"
                     /translation="MTLAELGMAFWHDLVAPVIAGILASMIVNWLNKRK"
     gene            16493..16600
                     /locus_tag="ACHOQQ_RS09590"
                     /old_locus_tag="pgaptmp_001918"
     CDS             16493..16600
                     /locus_tag="ACHOQQ_RS09590"
                     /old_locus_tag="pgaptmp_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4470176.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16976..17083
                     /locus_tag="ACHOQQ_RS09595"
                     /old_locus_tag="pgaptmp_001919"
     CDS             16976..17083
                     /locus_tag="ACHOQQ_RS09595"
                     /old_locus_tag="pgaptmp_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4470177.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17170..18849)
                     /gene="bcsG"
                     /locus_tag="ACHOQQ_RS09600"
                     /old_locus_tag="pgaptmp_001920"
     CDS             complement(17170..18849)
                     /gene="bcsG"
                     /locus_tag="ACHOQQ_RS09600"
                     /old_locus_tag="pgaptmp_001920"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGU4470178.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(18846..19037)
                     /gene="bcsF"
                     /locus_tag="ACHOQQ_RS09605"
                     /old_locus_tag="pgaptmp_001921"
     CDS             complement(18846..19037)
                     /gene="bcsF"
                     /locus_tag="ACHOQQ_RS09605"
                     /old_locus_tag="pgaptmp_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGU4470179.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(19034..20605)
                     /gene="bcsE"
                     /locus_tag="ACHOQQ_RS09610"
                     /old_locus_tag="pgaptmp_001922"
     CDS             complement(19034..20605)
                     /gene="bcsE"
                     /locus_tag="ACHOQQ_RS09610"
                     /old_locus_tag="pgaptmp_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGU4470180.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            20878..21066
                     /gene="bcsR"
                     /locus_tag="ACHOQQ_RS09615"
                     /old_locus_tag="pgaptmp_001923"
     CDS             20878..21066
                     /gene="bcsR"
                     /locus_tag="ACHOQQ_RS09615"
                     /old_locus_tag="pgaptmp_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HGU4470181.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         5302 a         5139 c         5592 g         5056 t
ORIGIN      
        1 aaagcatcac tatttgctaa ttaatgtgat ttaataaaat ggagtttttc atgaaaaagg
       61 tcgtttttgc cttaactgca ctggcgttaa cctccggctc tgcatttgca gcagaatctg
      121 gtgacggcac ggttaaattt acaggcgaga tcgtggattc accttgcgta ctgtccgttg
      181 actctcagaa ccaggaagtt gtgttgggtc aggtacaaaa atctgtattt gctgcggttg
      241 gtgacaaatc cccggcaaaa cctttcgaaa tcaaactgga agattgcgat accaccacga
      301 tgaaaaaagc caatgtgagc tttagcggtg ttggtgatgc agatcaatct gatctgattt
      361 ctgttagtac tgaagccggt gcggcgaaag gtgttggtat cggtatttat gataacagca
      421 acaccctagt cgctcttaac ggtggtaaag caagtgttga tctgagtaaa ggccagacgg
      481 ttctctactt taccgccaac tacgtttcaa ctctggcaac ggtaacgacc ggttacggca
      541 atgcgcaggt cgatttcaac ctgtcttacg agtaatcgtc tgttttaccc atggcggcgt
      601 aattgccgcc attagtcgtc aaaattcatg gatcgaatga tgaaaagtaa atttgttgcg
      661 ttggctctgt ctctgttcct ctcccagtca gttctggcgg ggggcgttgg gcttagtgct
      721 acccgtgtga tttatgatgg cagtaagaaa gaagcctcgc tgaccgtaca gaataaaagt
      781 aagacggaag agtttctgat ccagtcctgg gtggatgatg ccgcgggcag caaaaaaaca
      841 ccgtttataa ttaccccgcc gttgttcagg ctcgatcctg gaaaaaacaa tatattgcgt
      901 attgtaaata ctacccctgg cctgccgcag gatcgcgaat cggtctactg ggttaatgtg
      961 aaagccattc cgtctaaaag tgacgacagc gaaaacaaaa atgtattaca gattgctgtt
     1021 cgcacccgca tcaaactgtt ttatcgcccg gcagggttac agggtgatgt aaaaaccgcg
     1081 ccaaacgaac tgcgatttac gcgtagcggt aatcagatcc gtgtcgataa ccccaccgtt
     1141 tttaatatta cctttaatca attttttgcg aatgaaaaag aaattgaaaa agccggcatg
     1201 gtacccgcaa aagggtcatt aaatattaca ttacccgccg gggcaggtag cgtttcgaag
     1261 attaaatata acgccatcaa tgactttgga tcgtcagcgg aaatgatgac taaaaatgtt
     1321 gattgattaa taccttttca ggagaaattg ctcaatgtca cggaagaccg tttcacgtac
     1381 tttttcttcc ttttctatct ctgtggtggc ggttgctgtt gccagcacgt tttctgcaca
     1441 tgccggaaag tttaacccca aattcctgga ggacgttcag ggtgtgggcc agcatgtcga
     1501 tttaacgatg ttcgaaaaag gccaggaaca gcagttaccc ggcatctatc gtgtctctgt
     1561 ttacgtcaat gaacagagaa tggaaacccg cacactggaa tttaaagagg ccacagaagc
     1621 acagcgcaaa gcgatggggg aatcccttgt gccttgcctt agccggacgc aactggcaga
     1681 gatgggcgtg cgcgttgaaa gctttccggc cctcaatctg gtgcctgccg aagcctgcgt
     1741 gccgtttgac gaaatcattc cccaggcctc aagccatttc gacttcagtg aacaaaagct
     1801 ggtattaagt ttccctcagg cggcgatgca ccaggtggcg cgcggcacgg tgccggaatc
     1861 tttatgggac gaaggtatcc cggcattatt gctggattac agttttagcg ggagcaacag
     1921 tgaatatgac tcctcgggtt cttccagcag ttatgtcgat gataatggca ccgttcatca
     1981 tgatgatggc aacgatacgc taaagagcga cagctactac ctcaacttac gcagcggcct
     2041 gaaccttggc gcgtggcggt tacgtaatta cagtacctgg agccattccg gtggcaaagc
     2101 gcagtgggat aatatcggta cgtctttgag tagggccatc atccctttca aagcgcaatt
     2161 aacgatgggg gatacggcga ccgcggggga tatttttgac agcgttcaga tgcgtggcgc
     2221 gatgttagcc tctgatgaag agatgctgcc cgacagccag cgaggctttg cacccatcgt
     2281 tcgagggatc gccaaaagca acgccgaagt cagtattgaa caaaacggct acgttattta
     2341 tcgcacctat gtgcagcctg gtgcctttga aataaacgat ctttacccca ctgcaaacag
     2401 cggtgatctc acggtcatta ttaaagaagc ggatggcagt gaacaacgct ttattcagcc
     2461 attctcatcg gtgccaattt tccagcgtga aggccatctc aaatatagct ttgccgcagg
     2521 tgaatatcag gccgggaatt atgacagcgc ctcgccgcgt ttcgggcagc ttgatctgat
     2581 ctacggttta ccgtggggga tgacggccta cggcggcgta ttaatctcta ataattacaa
     2641 tgcatttgca ttagggatag ggaaaaactt tggttatatc ggggcgattt ccattgatgt
     2701 gacgcaggct aaaagcgaac tgaataacga tcgcgatagc cagggacaat cttatcgttt
     2761 cttatattcc aagagcttcg aaagcggcac cgatttccgc cttgcgggct atcggtactc
     2821 taccagcggt ttctatacct tccaggaagc caccgatgtg cgcagtgacg ctgacagcga
     2881 ctataaccgt tatcacaagc gcagcgaaat acagggtaac ctgacgcagc aattaggggc
     2941 ctatggctct gtttatttaa atttaacgca gcaggattac tggaacgacg caggtaaaca
     3001 gaacacggta tcggcgggtt acaacggacg tattggcaag gtcagttaca gtattgcata
     3061 tagctggaat aaaagccctg aatgggatga aagcgatcgc ttgtggtctt tcaatatttc
     3121 cgttccacta ggccgggcct ggagtaacta tcgcgtcacg accgaccagg atggtcgtac
     3181 caatcaacag gttggggtca gcggaacgct gcttgaggat cgcaacctga gctacagtgt
     3241 tcaggaaggc tacgccagca acggtgtggg taacagcggt aacgctaacg ttggctatca
     3301 gggtgggtcc ggtaatgtca acgtaggcta tagctacggg aaagattacc ggcagctcaa
     3361 ctacagcgtt cgcggcggcg tgatagttca tagcgaaggc gtgacgcttt cccaaccgct
     3421 aggcgaaacc atgacgctca tctccgtacc cggtgcgcgc aatgcccgcg tggtgaataa
     3481 cggcggcgtt caggttgact ggatgggtaa cgcgatcgtg ccttacgcca tgccgtatcg
     3541 tgaaaacgaa atctcactgc gtagcgattc gttgggtgac gatgttgacg ttgaaaatgc
     3601 gttccagaaa gtggtgccaa cgcgtggagc gattgtcaga gcgcgttttg atacccgcgt
     3661 tggttaccgc gtattaatga cgctgcttcg ttccgcgggc agcccggtgc cctttggagc
     3721 aacggcaacg ctaatcaccg ataaacaaaa cgaggtgagc agtatcgttg gtgaagaagg
     3781 acagctctat attagcggaa tgccagagga aggacgggta ttgattaaat ggggtaatga
     3841 cgcgtcgcag caatgcgtgg cgccttataa attatccctg gaattaaaac agggcggaat
     3901 tattcctgtt tcggccaatt gccagtaagc aaggagagcg aaatgaaagc ggcaatagca
     3961 ttatccttat taggatgcgt attcggattt tctggcaagg catttgccgg agatgcgtgg
     4021 gggccatgta cccccgcgga tggcacgact tatcactata atgtcgatgt ggatgttggt
     4081 atacctgatg cggcaaaaaa cgtcgcagga acggttttac cggatgtcct taactggtct
     4141 aatggacaaa acgtatcgct aatttgtgaa tgtcctgatt cttataagaa tgaaaaagat
     4201 actctagtac aaggggtgag tatgcttccc ccttctggcc gttcggttga cagtatgaaa
     4261 tattatactt tgacggagga actggaggtt gcgacgaaaa ttcgcatcag taccagtgtg
     4321 tatggatttg ttcctttcaa aaaccagcag gcgctgcaaa caaccggatg caacaaagtc
     4381 attactacgc cctatatggg cggcgcaggt ctactttcct ttgctattac taaacccttt
     4441 atcggcgatt ccgtcatccc tctgacatta attgccgaac tgtatgcctc caaaacaaat
     4501 aaagattacg gaacaatacc catatcttcc gtatctattc agggccgcgt caccataact
     4561 caggattgcg aaataaagcc cggcacggtg ctggatgtgc catttggtga attcccctcc
     4621 tcagcgttta aaaacagaca agggcaaatg cctgaaggtg cgacagagca agaaattaac
     4681 ctcggctttg attgcaacaa tatctcggat ggtatcaaag tctcgttgcg ccttgagggg
     4741 gcaaccaatg ctgacgatcc ccgagcggtg gatatgggca accccgatat tggcgtactg
     4801 gtgaaagatt ccagcggcaa aattttggta ccgaatgatg caagcagcac cacgttatta
     4861 aatttgagca gcctggattc taaaactcac cgcaatgccg cgattcgact gctggcttta
     4921 ccgattagca cgacgggtaa agcgcctaaa ggcgggacgt ttgaaggcgt aacgaccata
     4981 tatcttgaaa tggagtgatg ccatgaaatt caatctattg ctgcatagcg gcatcgccag
     5041 tttgagtctg tttgcctgcg gggtgaatgc ggcgacggat cttggcccgg caggggatat
     5101 tcatttctcc atcactatca ccactaaagc ttgcgagatg gaaaaaagcg atctcgaagt
     5161 cgatatggga acaatgacgc tgcaaaaacc tgcggcagtc ggtacggtgt tgagcaagaa
     5221 agatttcacc attgaactca aagagtgcga tgggatatcc aaagcgaccg ttgagatgga
     5281 cagtcagtcg gacagcgatg atgattccat gtttgccctt gaggctggtg gcgcaacggg
     5341 tgttgcgttg aagatagagg acgataaagg aacgcagcaa gttcccaaag gctccagcgg
     5401 aacgccgatt gaatgggcga ttgatggcga aaccacgtcg cttcactacc aggcgagtta
     5461 tgtggtcgtc aacactcagg ccactggtgg cacagcgaat gcccttgtca atttttccat
     5521 cacctatgag taagttactg ggccagcatc ctggcccaac ctatctctga atagtcgtta
     5581 atccgttaaa atggtctatt ccggacttgc acttttgtaa atttgtgctt catgcacact
     5641 ctttccccac actttttccc tttgctgtgg tctacttatt cgcgcgtgta gattttactt
     5701 atctgactac ctccgcactt ttcccctgct gggcctgaaa agccgctaag cagggtgtta
     5761 tcacctgttt gtccagggtt tgtttgcatg agatacatca aatcgattac acagcagaag
     5821 ctgagctttt tgcttgcaat ctatattggc ctttttatga atggcgcggt tttttaccgc
     5881 cgcttcggca gctatgcgca cgattttacc gtctggaaag gcatttctgc tgttgttgaa
     5941 ctggccgcca ccgtactggt gaccttcttt ttactacgtc ttctttcgct gtttggccgc
     6001 cgcagttggc gtattctggc atcgctggtt gtgctctttt ccgcaggtgc cagctattac
     6061 atgaccttcc ttaatgtggt cattggttat ggcatcatcg cttccgtcat gaccaccgat
     6121 atcgacctgt caaaagaagt tgttggtctg aactttattc tctggttaat cgccgttagc
     6181 gcattgcctc ttatccttat ctggaataac cgctgtcgct acaccttgct ccgacaactg
     6241 cgaaccccag ggcagcgtat tcgcagcctg gcggtcgtcg tactggcggg aattatggtt
     6301 tgggcaccga ttcgtttgct ggatatccag cagaagaaag tggagagggc gaccggcgtt
     6361 gatttgccga gttatggcgg tgtcgtagcg aactcttatc tgccatcaaa ctggctttct
     6421 gcgttggggc tgtacgcctg ggcgcgggtc gatgaatctt ccgataataa ttcattgctt
     6481 aacccggcga agaaattcac ttatcaggca ccgcaaaatg ttaatgacac ttatgtcgtg
     6541 tttatcatcg gtgaaactac gcgttgggac catatgggca ttttcggcta tgagcgtaat
     6601 accacgccga agctggccca ggagaaaaat ctggcggcgt tccgtggtta ctcctgtgat
     6661 accgcaacca aactctctct gcgttgcatg tttgtacgtc aggggggcgc ggaagataat
     6721 ccgcagcgca cattaaaaga acagaacatt ttcgcggtac tgaagcagtt aggattcagt
     6781 tctgacctct acgctatgca gagcgaaatg tggttctaca gcaatacgat ggcggacaat
     6841 attgcttacc gtgaacagat tggtgcggag ccacgtaacc gtggcaagcc ggtagatgat
     6901 atgttgctgg tagacgaaat gcagcaatcg ctggggcgca acccggatgg taagcatcta
     6961 atcattctgc ataccaaagg ttcgcacttt aactacaccc agcgttaccc gcgcagcttt
     7021 gcgcagtgga agccggaatg tattggtgtt gatagcggat gtaccaaagc gcagatgatc
     7081 aactcctatg acaactcggt gacctatgtg gatcacttta tctccagtgt gattgatcag
     7141 gttcgcgata agaaagcgat tgtgttctac gcagctgacc acggcgagtc aattaatgaa
     7201 cgcgagcacc tgcacggcac gccgcgtgaa ctggcaccgc cggagcagtt ccgcgtaccg
     7261 atgatggtct ggatgtcaga taaatatctg gaaaatccgg tcaatgcgca ggcgtttgcg
     7321 cagctgaaaa aagcagccga catgaaagtg ccacgccgtc acgtagagct gtacgacacc
     7381 atcatgggtt gtcttggcta tacttcaccg gatggtggaa ttaacgaaaa caacaactgg
     7441 tgtcacatcc cgcagacaaa agaggcagtg gctaactaag aggcctgctg actttctcgc
     7501 cgatcaaaag gcattttgct attaagggat tgacgagggc gtatctgcgc agtaagatgc
     7561 gccccgcatt cggtgattgg cgcagcctgg tagcgcactt cgttcgggac gaaggggtcg
     7621 gaggttcgaa tcctctatca ccgaccaaat tcgaaaagcc tgctcaacga gcaggctttt
     7681 ttgcatctgc agcccatgag gaagagaacc tccgggggca gggaggttcg actcgacgta
     7741 ggcctgataa gacgcgtcag cgtcgcatca ggcaacggca ccgcacttat tgtcggatgc
     7801 ggcgtgaacg ccttattcga cctacacacc acacatttcc ctgccaaatt tgcgccccat
     7861 atctcactat ccattaacgg atttgtgaca gaaaccattg tcttttttta tatttgctta
     7921 aatctttttt tgcgttactt atggctggcg ctggcatttt ccgctgtgcg ttttagcgtt
     7981 catggtatta atcgctcaga tatccacctt ttcctcagat ttttttcatt aaatgcataa
     8041 aacttaatca ccaaatgttg ctaaatatta agctgattgt tctggtcatg ttggagtagc
     8101 acaaatttct ctgctgcaaa tagctgtttg aagttttttt aatctttgtt tgtggattct
     8161 caccgttggt gtaaatcccg gttggctgta ttgacgtttt cacattctgt tgacagattg
     8221 taggtcacga ggggcatttt atggaggatc cgcactgtta cactgatgtt aattagtacg
     8281 gcatccccac ctcataacgt tgacccgacc gggcaaaaaa caaaaaaggt caggcagcga
     8341 caacccactg caaagggtta aaacaacaaa catcacaatt ggagcagaat aatgcgtatt
     8401 tccttgaaaa agtcagggat gctgaagctt ggtctcagcc tggtggctat gaccgtcgca
     8461 gcaagtgttc aggctaaaac tctggtttat tgctcagaag gatctccgga agggtttaac
     8521 ccgcagctgt ttacctccgg taccacctat gacgcctctt ccgtaccgct ttataaccgc
     8581 ctggttgaat ttaaaatcgg caccaccgaa gtgatcccgg gcctcgctga aaagtgggaa
     8641 gtcagcgaag acggtaaaac ctataccttc catctgcgta aaggtgtgaa gtggcacgac
     8701 aataaagaat tcaaaccgac gcgcgaaatg aatgccgatg acgtggtgtt ctcgttcgat
     8761 cgtcagaaaa acgcgcaaaa cccgtaccat aaagtttctg gcggtagcta cgaatacttc
     8821 gaaggcatgg gcttgccgga gctgatcagc gaagtgaaaa aggtggacga caacacagtt
     8881 cagttcgtgc tgactcgccc ggaagcgccg ttcctcgctg acctggcaat ggacttcgcc
     8941 tctattctgt caaaagaata tgctgacgcg atgatgaaag ccggtacacc ggaaaaactg
     9001 gacctcaacc caatcggaac cggtccgttc cagttacagc agtaccaaaa agattcccgt
     9061 atccgctaca aagcgtttga tggctactgg ggcaccaaac cgcagatcga tacgctggtt
     9121 ttctctatta cccctgatgc ttccgtgcgt tacgcgaaat tgcagaagaa tgaatgccag
     9181 gtgatgccgt acccgaaccc ggcagatatc gctcgcatga agcaggataa atccatcaac
     9241 ctgatggaaa tgccggggct gaacgtcggt tatctctcgt ataacgtgca gaaaaaacca
     9301 ctcgatgacg tgaaagttcg ccaggctctg acctacgcgg tgaacaaaga tgccatcatt
     9361 aaagcggttt atcagggcgc gggcgtggca gcgaaaaacc tgatcccgcc aaccatgtgg
     9421 ggctataacg acgacgttca ggactacacc tacgatcctg aaaaagcgaa agccttgctg
     9481 aaagaagcgg gtctggaaaa aggtttctcc atcgacctgt gggcgatgcc ggtacaacgt
     9541 ccgtataacc cgaacgctcg ccgcatggcg gagatgattc aggcagactg ggcgaaagtg
     9601 ggcgtgcagg ccaaaatcgt cacctacgaa tggggtgagt atctcaagcg tgcgaaagat
     9661 ggcgagcatc agacggtaat gatgggctgg actggcgata acggggatcc ggataacttc
     9721 ttcgccaccc tgttcagctg cgccgcctct gaacaaggct ccaactactc aaaatggtgc
     9781 tacaaaccgt ttgaagatct gattcaaccg gcgcgtgcta ccgacgacca taacaaacgc
     9841 gttgaactgt acaagcaagc gcaggtggtg atgcacgatc aggctccggc actgatcatc
     9901 gctcactcca ccgtgtttga accggtacgc aaagaagtta aaggctatgt ggttgatcca
     9961 ttaggcaaac atcacttcga aaacgtctct atcgaataat taaaagccat acaagactga
    10021 tggcaaaggc aaaactgcct gatgcgctcc gcttatcagg cctacgaaaa ttctgcaatg
    10081 tattgagttt gcaagatttt gtaggccgga taaggcgtta acgccgcatc ccgcataaac
    10141 aaagcgctct ttgtcaacaa tctgcatacc cggtggtgct gtgcctctgc gtgcagcgcc
    10201 atccggcagc aatacttcat tccctgtcag acaagggcgg gaattgattt gtgagcaata
    10261 cagacacgca gttccaggct gcgggtcact acagagaatc cgggttatgt tgcagtttat
    10321 tctccgacgt ttgggactcg tcatccccac gtttatcggt attacccttc tcacatttgc
    10381 ctttgtccac atgatcccgg gcgatccggt gatgatcatg gcgggcgagc gtgggatctc
    10441 cccagagcgt cacgcgcagc tgctggctga actcggctta gataaaccga tgtggcagca
    10501 gtatctccat tacatttggg gcgttatgca tggcgatcta ggcatttcaa tgaaaagccg
    10561 catcccggtt tgggaagagt tcgtgccgcg cttccaggcc acgctggaac ttggcgtctg
    10621 cgcgatgatt tttgctacgg cagtcggtat tccggttggt gtgctggctg cggttaaacg
    10681 cggttccatt ttcgatcaca cagcggttgg cctggcgctg acaggttatt caatgcctat
    10741 cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg cactggaacc tgacacccgt
    10801 ctccggtcgc gtgagcgata tggtgttcct cgatgattcc aatccgttaa ccggttttat
    10861 gctgatcgac accgccatct ggggtgaaga cggtaacttt atcgatgccg tcgcccatat
    10921 gatcttgccc gccattgtgc tgggtactat tccgctggcg gtcattgtac gtatgacacg
    10981 ctcctcgatg ctggaagtgc tgggtgaaga ttacatccgc accgcgcgcg ccaaaggatt
    11041 gacccgcctt agggtgatta tcgtccatgc gctgcgtaac gcgatgctgc cggtagtgac
    11101 cgttatcggc ctccaggtgg gaacattgct ggcgggggcg attctgaccg aaaccatctt
    11161 ctcgtggccc ggtctggggc gctggttgat tgacgcactg caacgccgcg actatccggt
    11221 agtgcagggc ggcgtattgc tggtggcgac gatgattatc ctcgtcaatc tgctggtcga
    11281 tctgctgtac ggcgtggtga acccgcgtat tcgtcataag aagtaagggg acatcatgtc
    11341 acaggttact gaaaataaag tgattagcgc accggtgccg atgaccccgt tacaggagtt
    11401 ctggcactat tttaaacgca acaaaggcgc ggtcgtcggg ctggtttacg tcgtcatcgt
    11461 gctgtttatc gcgatctttg ccaactggat tgctccctat aacccggcgg aacaattccg
    11521 cgatgcgctg cttgccccgc ccgcctggca ggaaggcggc agcatggcgc acttgctggg
    11581 caccgatgac gtaggccgtg atgtgctgtc gcggctgatg tacggcgcgc gtttatcgct
    11641 gctggttggc tgtctggtgg tggtgttatc gctgattatg ggcgttattc tcggcctgat
    11701 cgccggttac tttggcggcc tggtcgataa catcatcatg cgcgtggtcg atatcatgct
    11761 ggcgctgcca agtctgctgt tggcactggt gctggtggcg attttcggcc cgtcgattgg
    11821 taacgccgcg ctggcgctga ccttcgttgc cttgccgcac tatgtgcgct taacacgcgc
    11881 cgccgtgctg gtggaggtga atcgcgatta cgttaccgcg tctcgcgtgg caggtgccgg
    11941 ggcgatgcgt cagatgttta ttaacatctt cccgaactgc cttgcgcctc tgattgttca
    12001 ggcgtcgctc ggtttctcta acgccattct cgatatggct gctcttggtt tcctcggcat
    12061 gggggcgcag ccgccaacgc cggagtgggg caccatgctc tccgacgtgt tgcagttcgc
    12121 gcaaagcgcc tggtgggtcg tgaccttccc gggtctggcg atcctgctga cggtgctggc
    12181 atttaacctg atgggtgacg gtctgcgtga cgcgctcgat cccaaactga agcagtaaga
    12241 ggttcgagat ggcgttatta aatgtagata aattatcggt gcatttcggc gacgaaagcg
    12301 caccgttccg cgccgtagac cgcattagct acagcgtgaa acagggcgaa gtggtcggga
    12361 ttgtgggtga gtccggctcc ggtaagtcgg tcagttcact ggcgattatg gggctgattg
    12421 attatccggg ccgcgtgatg gcggaaaagc tggagtttaa cggtcaggat ttgcagcgta
    12481 tctcagaaaa agagcgccgc aatctggtgg gtgccgaagt ggcgatgatc ttccaggacc
    12541 cgatgaccag ccttaacccg tgctacaccg ttggtttcca gattatggaa gcgattaagg
    12601 tgcatcaggg cggtaataag agcactcgcc gtcagcgggc gattgacctg ctgaatcagg
    12661 tcggtattcc cgatccggca tcgcgtctgg atgtttaccc gcatcagctt tccggcggca
    12721 tgagccagcg cgtgatgatt gcgatggcca ttgcctgtcg gccaaaactg ctgattgccg
    12781 atgaaccgac cactgcgctg gacgtgacca ttcaggcgca aatcatcgaa ctactgctgg
    12841 agctacagca gaaagagaac atggcgctgg tgttaattac ccatgacctg gcgctggtgg
    12901 cggaagcggc acataaaatc atcgtgatgt atgccggtca ggtggtggaa acgggcgatg
    12961 cgcacgccat cttccatgcg ccgcgtcacc cgtatactca ggcattgctg cgtgcgctgc
    13021 cggaatttgc tcaggacaaa gaacgtctgg catcgttgcc tggcgttgtt cccggcaagt
    13081 acgaccgccc gaacggctgc ctgcttaacc cgcgctgccc ctatgccact gacagatgtc
    13141 gcgctgaaga accggcgctg aatatgctcg ctgacgggcg tcagtccaaa tgccattacc
    13201 cacttgatga tgccgggagg ccgacactat gagtacgcaa gaggccacct cgcaacaacc
    13261 gctgttgcag gctatcgacc tgaaaaaaca ttatccggta aagaaaggta tgttcgctcc
    13321 ggaacgtctg gtgaaggcgc tggacggcgt ttcgtttaac cttgaacgtg gcaaaacgct
    13381 ggcggtagta ggtgaatctg gctgcggtaa atcgaccctc ggtcggttgc tgacgatgat
    13441 tgaaacgccc accggcggcg agctgtatta ccaggggcag gatctgctta agcacgatcc
    13501 gcaggcgcag aagctgcgtc ggcagaaaat ccagattgtc ttccagaacc cttacggttc
    13561 gctgaatccg cgtaaaaaag tcgggcaaat tcttgaagag ccgctgctga tcaacaccag
    13621 cttaagcaaa gaacagcgtc gggaaaaagc cctgtcgatg atggcgaaag tcggcctgaa
    13681 aaccgagcac tatgaccgct atccgcatat gttctccggc ggacaacgtc agcgtatcgc
    13741 tattgcccgt ggtctgatgc tcgacccgga cgtggtgatt gccgatgaac cggtctccgc
    13801 gctggacgtc tcggtgcgtg cacaggtgct caacctgatg atggatttgc agcaggaact
    13861 ggggctttct tatgtcttca tctcccacga cctgtcggtg gtggagcaca ttgctgatga
    13921 agtgatggtg atgtacctgg gccgctgcgt ggagaaggga acgaaagacc aaatcttcaa
    13981 taacccgcgc catccgtaca ctcaggcgct actttccgcg acgccgcgcc tgaatccgga
    14041 cgatcgccgc gagcgcatca aactcaccgg tgaactgcca agcccgctga atccaccgcc
    14101 gggctgcgcg tttaacgccc gctgtcgtcg gcgcttcggc ccctgcaccc agttgcagcc
    14161 gcagctaaaa gactacggcg gtcaactggt agcttgtttt gctgttgatc aggatgaaaa
    14221 tccgcagcgt taactcacaa aaccggggcc atgcgctccg gtttactcaa tcagcttcaa
    14281 cagtggcgac aagcatagca aaatgccgta aagcagaatc agccaggctt taaatccgcg
    14341 aagtttttcc agttgtgcga ctttatagat caggaaaaac ggaatgaggc acgacacaat
    14401 gccatataac gggcttccca actgaaagaa caccagcacc gatacacgaa acgaaaccca
    14461 aatcgtcaac gtaatgacga taaaagcgca gatcgccaga gtcagcacgc gtgagttaat
    14521 tttcttggta tcaataattc ggcttaacag attgagaata atgcctttaa tggcctcgtg
    14581 gaaaccgaga taaatgccaa agaatgcggt cagtacggca aagatattca gcaccgtaga
    14641 ggtgatatga atgatatgcc cagggatcac ctgcgcggcc agtgccagtg ccgagatatt
    14701 ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag aaggtaaacg aaaaggcaaa
    14761 aaacagtatc accgcgatga gcgtgatata actaattcgg tgggtacgca gcgcgagccg
    14821 ggttgccagt actttatccg cttcccgttt acgataggca atattcattg ggttaagtac
    14881 ctgaataaat actgcagaaa agaagcaaaa tggaatggta agcaggacat cgcgaaaaaa
    14941 gacggaggct tgcgggaagg cggttatatt ggcgaaattc cagtgcggga tcatcgcaaa
    15001 accgaacacg acaataatcc ctactttgac caccaccatt ggcccagaaa tcttaaacag
    15061 taatcgttca ccaccagacg caatcgctac cagtacggcg aaaatagcga ctttataaag
    15121 tagagattgt gaaagatcgg catcggttaa accgaaggtt ttcaggtacg aggcgctgtc
    15181 gaaaaccacg gagagagagt agataaaaat cccgtggata atcatcaaaa agtagataac
    15241 cccgaggaaa attccccagt tcttccccag gtaatgactg ataatatcgg tgtagtcatt
    15301 acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc tgcactaccc aggtggcagg
    15361 ataagcaatg atcgctgcgg taataaatac ccaaataccc ttcaagccaa tttgtactgg
    15421 catcagcacg gttccggcac caatcgccat gcctatgcat aataaaaccc agccgaagtc
    15481 gtagcgtgta aacggcagtt tctgatcgtg tttcggtagt gtgttgtgct gcataaagtc
    15541 ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc cagtttctct tgcgcaacag
    15601 gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa cagattcact ggggaggtcg
    15661 cgaggaggcg caattctaca aaattgcgca tttcactacc agtctggagt cagcccggat
    15721 atgaactgca actattgcaa ataaagaaat taagctggaa ggcatcgcgc aatgaggaag
    15781 attgccgggt gctgacagta aaaagcaatc gtaaaatcag cggcaagccg gaagggttcc
    15841 ggtgaggcgc aatgttgcgg gggctttatc cctggtggca ttggttgctg gagagagaaa
    15901 acccccgcac gttgcaggta tgcacctgac aacataacgg gggctaatct tgaacctaga
    15961 ccactcaaga atagccgcga aacgttgtca ttacaacaca ggcggctata tgacgctcgc
    16021 agagctgggc atggccttct ggcatgattt agtggctccg gtcattgctg gtattcttgc
    16081 cagtatgatc gtgaactggc tgaacaagcg gaagtaatgt gtcatgcggg cgtcaggctg
    16141 ccgtaatggc aatttgcgcc cggaccaggc cgcaggggtg aaactctgcg gcctttttcg
    16201 taagaaaaat gcaacggttg caagcggata atattctgcg gaatgcgcag cacagagtga
    16261 agacgtgacg gattccggaa cagaaaaaca gtcgtagaat cagcgtcaag ccggaaggat
    16321 tccggtgagg cgcaatgttg cgggggctta atccctggtg gcattggttg ctggagagag
    16381 aaaacccccg cacgttgcag gtatgcacct gacaacatca cgggggccat tcttgaacct
    16441 gaacaactca agaatagccg cgaaacgttg tcattacaac acaggcggct atatgacgct
    16501 cgcagaactg ggcatggcct tctggcacga tttagcggct ccggtcattg ctggcattct
    16561 tgccagtatg atcgtgaact ggctgaacaa gcggaagtaa cgtgtcatgc gggcgtcagg
    16621 ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg gtgaaactct gcggcctttt
    16681 tcgtaagaaa aatgcaacga ttgcaagcgg ataatattct gcagaatgcg cagcacagag
    16741 tgaagacgtg acggattccg gaacagaaaa acagtcgtag aatcagcgtc aagccggaag
    16801 gattccggtg aggcgcaatg ttgcgggggc ttaatccctg gtggcattgg ttgctggaga
    16861 gagaaaaccc ccgcacgttg caggtatgca cctgacaaca tcacgggggc cattcttgca
    16921 tctagaccat tcaagaatag ccgcgaaacg ttgtcattac aacacaggcg gctatatgac
    16981 gctcgcagag ctgggcatgg ccttctggca tgatttagcg gctccggtca ttgctggcat
    17041 tcttgccagt atgatcgtga actggctgaa caagcggaag taacgtgtca gacgggcgtc
    17101 aggctaccgt aatggcaatt tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct
    17161 ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg ttcaggcgaa cgtacggttt
    17221 atcctggtat tgaataacta ctgcatttga gttctcggag accggtgctg tttgtggcaa
    17281 cccactggtg agttttttcc agtcaacatt gtcttcggtg aaaatcttgc catcaagaac
    17341 gcgaaccacc agatcggaga tagccaggaa gctgctcggt tggtcgatga caatcggtgc
    17401 cccctgatgt ggtgccttca tgccgaagaa tttcacccca acggggacgt cggtgataga
    17461 cgggctaggg atatcacgta ggccagatac ctgcattctg tcgcccttca gcgcgccacc
    17521 gtgttccggc accacgacca ccatcacttt acgacccgat ttctccagtt cagtaaagaa
    17581 ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg taatccgctg ttttgctgac
    17641 gcccggataa tggttgccgt catgcagtgg aagcgtgttg tagaacgtgg cactacggct
    17701 gtttttatct ttttcggtaa cgtccagcca gcggttaagc acggcggtat cgtcataaac
    17761 cggcgaacca tcaaagccca gcaaaataac cggcagattt gtttgatcca tcaattcgct
    17821 ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg ccgaactggc cgttatgtcc
    17881 catcatcagg tgctgggtaa agcccagttt cgacaggtta tcaaacagat agcagtcgtt
    17941 atttgccggt tgatacagat tagtgtgcga agtctgcccg cagctggcgc gcagtaaacg
    18001 gatcgccgcc gggccactgt aggaggtggc ggagttaaag ttcttgaact caatatcgaa
    18061 atgcgaccac agcggatgcg acatcaaccc ggcggcttca atatccgacc aggaaagcga
    18121 acaaatatta atcaccagta gttcaaatgg ctgagcatca gcgggcagcg aagacgggaa
    18181 gatcgattta cgtttcgcct ccgcgttata gaaattattc agccaggcgt taaggttcgc
    18241 cgttgttggc ggtgcagttt gtgcgggcat atcacccact accggtgcgc cacccgtcgc
    18301 cgcaacggtt gccgctgcgt taccacccgt cgttgttaca gtggtcgtcg gttgaccggc
    18361 tggccacaag gagaaacttg gtcccgccag ggtaagtacg ttcagccaca gcagaatggc
    18421 aaccacaaaa acggtaatgc gaatccattg tgacaggaat aaccaggcca ctaataaaac
    18481 aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg acaaggtcga ttaaataatc
    18541 ggtactgaac cccgccacct gcgaaccctg gctcattatg ctttccgggc caggcaacca
    18601 ggtgtcatgc cagaacaaag caaagccgat cggcagggca atccagtggc gcaagcgatg
    18661 caggctgtag cgcggaatgg gcatcagcag aaacgcggca aacaccaaat tgaggagcgg
    18721 atggaagtta agatatcccg cccacaacag gccgaactta accagaaaat agaagttcca
    18781 gccggaaagg ccgcgccagt attgccagag ggaagaaggc atggcggtat tttgcgtaaa
    18841 ttgagtcatt ttttggttgc cctggctttt tccgtgcggc gtaacgtccc agccggttta
    18901 acataacgag gtttagcaaa gaacaaacgt aaggtgtcgc gaatgcgtcg caaagagtgc
    18961 cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa cgacaataat ttcaatgata
    19021 tcgctgatgg tcatcatgat gagcgctcca cagcatcatc taacagtcgc atgggttctg
    19081 gtattcgtcg ccagtggcga ccatcgtgct cggcattgat gaccggttta gaactttgtg
    19141 ttaaaggcag cggcatgccc cattgttctg gggcaagcag gcgcatctgc accagctcgg
    19201 cactgatttg atcatcttca aaccagacca tacggtttga gaaaatgtcg ccagtaggca
    19261 atgggaaaat atgattcaac gcggtatcca gatcgttaat ccgacagaat gagagaaaca
    19321 gcaccagccg attaccgcca atggtcatga tatcgccggt acggttaggg cgacatagcg
    19381 tcagggcttg ctcaacgcgg atacccggta ccggacgtag ggcaaccaga acacctttac
    19441 cgtgggcagg caaaagaggg ttattcatca tgttgttgac ggcattacag aacacatccc
    19501 acttctggaa accacgcagt ttgagcggct gggtcattga cagcaaggta gtgatatctt
    19561 ccggcacata gcgactaaac ttctgccctt gcacgctttc gatcatcgtc agacagcggg
    19621 agagtggcgc attccacggg ataaccatat ttgcaccgca ggccaataac aaacgttcat
    19681 cggtggcgcg caggctagcg gtattttccc gtacgaggat tttcatcgca ctaccgcgct
    19741 ggcgacgcag agtatgaatg ctgcgggcca gtggctcgat ttgcgcattt tgttgtaaag
    19801 aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt gaacaggact tcgttattgt
    19861 tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc cagtacagca acattactca
    19921 gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc ttcgctttga actaatgtcc
    19981 agataccatt ttgttgctga acgctaagct gctgacgggc gctgacccct ttttcgttgc
    20041 accagaaggc aatatccagc aaatgttggt cgccctgaaa acgcaaactg gcaagaccaa
    20101 aaagtgaacg gtattcctca agcaacaatg aaaattgttt atcgttatta tttccgggat
    20161 taattaccaa aagcgaacaa tggtttaacc tgctccattt attcatttta tccaaccatg
    20221 agcgaagccg ctcggcagga atgttttgcc atgcgttatt tgcgcaaaca agaataaaaa
    20281 ggtaattatg gggatcaata gaacactgca aatcacgggc caaatagtat agaccttttt
    20341 catgatttag cattgaaaat aattttattt tttccggccc ttgagaatca tctaattgaa
    20401 agattttcgc cggatcgctg tccatgctaa tgaccgcgac gtgtgcggtt tcagcctggg
    20461 atgcaattgt ttgattcgcc agactgatag catcttcatg gcgatcgacg ttaaaccacc
    20521 agacgccgcc tgctggcata tgtcgcagct catcccataa tgatgagata ccgatagaga
    20581 atacagggtc cacaatgtcc ctcatgtcac cattttgtct gattatcagt ttactagcga
    20641 aagctaagaa ataaacctac cattgaaatt aaaacttatc agatttagca tgtgaatcaa
    20701 tttagcaggt cacaagacgt gtgattttag tcttttcgtt tttaactttt tctgttgcgt
    20761 aacggaaagt caaaaagtga gcaaattccc gtctcgccgt aaacaataaa ccggcgaaat
    20821 gctcacggtt agaattgtct catgaacggt acggttattt catagggatc aagcaaaatg
    20881 aataacaatg aaccagatac tctgcctgat cccgcgatag gctatatctt ccagaatgat
    20941 attttggcgt taaagcaggc attttcactg cctgatattg attatgccga tatttcccaa
    21001 cgcgaacagt tggccgcggc attaaaacgc tggccgttgc tggcagagtt tgcgcaacaa
    21061 aagtagggga ttggtgaatg gccgtactg
//
LOCUS       DAZKFU020000066        20919 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZKFU020000066 DAZKFU020000000
VERSION     DAZKFU020000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.066"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..298
                     /gene="ytcA"
                     /locus_tag="ACHOQQ_RS09620"
                     /old_locus_tag="pgaptmp_001924"
     CDS             23..298
                     /gene="ytcA"
                     /locus_tag="ACHOQQ_RS09620"
                     /old_locus_tag="pgaptmp_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGU4470182.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            317..1348
                     /gene="mdtN"
                     /locus_tag="ACHOQQ_RS09625"
                     /old_locus_tag="pgaptmp_001925"
     CDS             317..1348
                     /gene="mdtN"
                     /locus_tag="ACHOQQ_RS09625"
                     /old_locus_tag="pgaptmp_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGU4470183.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1348..3399
                     /gene="mdtO"
                     /locus_tag="ACHOQQ_RS09630"
                     /old_locus_tag="pgaptmp_001926"
     CDS             1348..3399
                     /gene="mdtO"
                     /locus_tag="ACHOQQ_RS09630"
                     /old_locus_tag="pgaptmp_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGU4470184.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGVQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQVLNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICHTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATGSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLTVALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            3396..4862
                     /gene="mdtP"
                     /locus_tag="ACHOQQ_RS09635"
                     /old_locus_tag="pgaptmp_001927"
     CDS             3396..4862
                     /gene="mdtP"
                     /locus_tag="ACHOQQ_RS09635"
                     /old_locus_tag="pgaptmp_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGU4470185.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKNSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVLQSIQLMKSLGGGYQAGPVVEKK"
     gene            5060..7207
                     /gene="fdhF"
                     /locus_tag="ACHOQQ_RS09640"
                     /old_locus_tag="pgaptmp_001928"
     CDS             5060..7207
                     /gene="fdhF"
                     /locus_tag="ACHOQQ_RS09640"
                     /old_locus_tag="pgaptmp_001928"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5477..5479,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HGU4470186.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7301..7990
                     /gene="yjcO"
                     /locus_tag="ACHOQQ_RS09645"
                     /old_locus_tag="pgaptmp_001929"
     CDS             7301..7990
                     /gene="yjcO"
                     /locus_tag="ACHOQQ_RS09645"
                     /old_locus_tag="pgaptmp_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGU4470187.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(8295..9608)
                     /gene="gltP"
                     /locus_tag="ACHOQQ_RS09650"
                     /old_locus_tag="pgaptmp_001930"
     CDS             complement(8295..9608)
                     /gene="gltP"
                     /locus_tag="ACHOQQ_RS09650"
                     /old_locus_tag="pgaptmp_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGU4470188.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9950..10546)
                     /gene="nrfG"
                     /locus_tag="ACHOQQ_RS09655"
                     /old_locus_tag="pgaptmp_001931"
     CDS             complement(9950..10546)
                     /gene="nrfG"
                     /locus_tag="ACHOQQ_RS09655"
                     /old_locus_tag="pgaptmp_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGU4470189.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10543..10926)
                     /gene="nrfF"
                     /locus_tag="ACHOQQ_RS09660"
                     /old_locus_tag="pgaptmp_001932"
     CDS             complement(10543..10926)
                     /gene="nrfF"
                     /locus_tag="ACHOQQ_RS09660"
                     /old_locus_tag="pgaptmp_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGU4470190.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10919..12637)
                     /gene="nrfE"
                     /locus_tag="ACHOQQ_RS09665"
                     /old_locus_tag="pgaptmp_001933"
     CDS             complement(10919..12637)
                     /gene="nrfE"
                     /locus_tag="ACHOQQ_RS09665"
                     /old_locus_tag="pgaptmp_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGU4470191.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGVVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(12657..13613)
                     /gene="nrfD"
                     /locus_tag="ACHOQQ_RS09670"
                     /old_locus_tag="pgaptmp_001934"
     CDS             complement(12657..13613)
                     /gene="nrfD"
                     /locus_tag="ACHOQQ_RS09670"
                     /old_locus_tag="pgaptmp_001934"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGU4470192.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13610..14281)
                     /gene="nrfC"
                     /locus_tag="ACHOQQ_RS09675"
                     /old_locus_tag="pgaptmp_001935"
     CDS             complement(13610..14281)
                     /gene="nrfC"
                     /locus_tag="ACHOQQ_RS09675"
                     /old_locus_tag="pgaptmp_001935"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGU4470193.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(14278..14844)
                     /gene="nrfB"
                     /locus_tag="ACHOQQ_RS09680"
                     /old_locus_tag="pgaptmp_001936"
     CDS             complement(14278..14844)
                     /gene="nrfB"
                     /locus_tag="ACHOQQ_RS09680"
                     /old_locus_tag="pgaptmp_001936"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGU4470194.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14889..16325)
                     /gene="nrfA"
                     /locus_tag="ACHOQQ_RS09685"
                     /old_locus_tag="pgaptmp_001937"
     CDS             complement(14889..16325)
                     /gene="nrfA"
                     /locus_tag="ACHOQQ_RS09685"
                     /old_locus_tag="pgaptmp_001937"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGU4470195.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16718..18676
                     /gene="acs"
                     /locus_tag="ACHOQQ_RS09690"
                     /old_locus_tag="pgaptmp_001938"
     CDS             16718..18676
                     /gene="acs"
                     /locus_tag="ACHOQQ_RS09690"
                     /old_locus_tag="pgaptmp_001938"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGU4470196.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18809..19123
                     /gene="yjcH"
                     /locus_tag="ACHOQQ_RS09695"
                     /old_locus_tag="pgaptmp_001939"
     CDS             18809..19123
                     /gene="yjcH"
                     /locus_tag="ACHOQQ_RS09695"
                     /old_locus_tag="pgaptmp_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGU4470197.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            19120..20769
                     /gene="actP"
                     /locus_tag="ACHOQQ_RS09700"
                     /old_locus_tag="pgaptmp_001940"
     CDS             19120..20769
                     /gene="actP"
                     /locus_tag="ACHOQQ_RS09700"
                     /old_locus_tag="pgaptmp_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGU4470198.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGCWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         4951 a         5719 c         5546 g         4703 t
ORIGIN      
        1 cagcagacag gatgtattta atatgccaac agttctctct cgcatggcga tgcaactcaa
       61 aaaaacagcc tggataattc ccgtcttcat ggtttcggga tgttcattat ctccggcaat
      121 cccggtgatc ggcgcttatt atcccagctg gtttttctgc gccattgcca gccttatttt
      181 gacgctcatc acgaggcgaa ttattcagcg gacaaatatc aatctggcat ttgtcggaat
      241 tatttatacc gccctttttg ctctctacgc catgctgttc tggctggcat ttttctaatc
      301 tatttgagac gcgctcatgg aaagtacgcc gaaaaaagct cctcgcagta aattccctgc
      361 tctgttagtg gttgcgttgg cgctggttgc ccttgttttc gttatctggc gcgtagacag
      421 tgcgccatca actaatgacg cttacgcgtc agcagatacc attgatgtgg tgccggaagt
      481 cagcggccgc attgtagaac tggcggtcac cgacaaccag gcagtcaaac agggcgattt
      541 gctgttccgc attgacccgc gcccgtacga agccaatctg gcgaaagctg aagcctccct
      601 cgcggcactg gataagcaaa ttatgctcac ccagcgtagc gttgacgcgc aacagtttgg
      661 tgccgactcg gttaacgcca cggtagaaaa agcccgtgcc gccgctaaac aggccacaga
      721 tacattacgc cgcaccgagc cattactgaa agaaggtttt gtctcagcgg aagacgttga
      781 ccgtgcgagg accgcgcagc gcgctgcaga agcggatctt aatgccgtat tgttacaggc
      841 gcagtcagcc gccagcgccg tcagcggcgt cgatgcgctg gtggcgcaac gtgcggcggt
      901 cgaagcggat attgccctga ccaaactgca tctggaaatg gcgaccgttc gcgcaccgtt
      961 tgatggccgg gtcatttccc tcaaaacctc cgtcgggcaa tttgcttctg ccatgcgccc
     1021 tatttttacc ctaatcgaca ctcgtcactg gtacgtgatc gccaacttcc gcgaaaccga
     1081 tctgaaaaat attcgctcag gtacacccgc aacgattcgc ctgatgagtg acagcggcaa
     1141 aactttcgag ggtaaagtgg attcgattgg ctacggcgtg ctaccggatg acggcggcct
     1201 ggtgctgggc ggcctgccaa aagtttctcg ctctattaac tgggtccgcg ttgcccagcg
     1261 ttttccggtc aaaatcatgg tcgataaacc tgacccggaa atgttccgca tcggcgcttc
     1321 ggcagtcgct aatcttgagc cgcaataatg agcgcgctca actccctgcc attaccggtg
     1381 gtcaggctgc tggcattctt tcatgaagag ttaagcgagc ggcgaccagg tcgcgtgccg
     1441 cagaccgtgc aactctgggt aggctgcctg ctggtgattc tgatctcgat gacttttgag
     1501 atcccttttg tggcgttatc gctggcagtg ctgttttacg gtgttcagtc gaacgcgttt
     1561 tacaccaaat ttgtcgcgat cttgtttgtg gttgccacgg tgctggagat cggcagcctg
     1621 tttttgatct acaaatggtc atacggcgaa ccgttgatcc gcttgatcat cgccgggccg
     1681 atcctgatgg gctgtatgtt tttgatgcgc acccatcggt tggggctggt ctttttcgcc
     1741 gtcgccattg tcgctattta cgggcaaacc ttccccgcca tgctcgacta tccagaagtg
     1801 gtcgtgcgct taacgctgtg gtgtatcgtt gttggcctct atccaacctt gctgatgacg
     1861 ttaatcggcg tactgtggtt tcccagtcgt gccatttcgc aaatgcatca ggtgcttaat
     1921 gatcggcttg atgatgccat tagccacctg acggacagcc tcgcaccgct acccgaaacg
     1981 cggattgaaa gagaggcgct ggcgctgcaa aaactcaatg tcttttgcct cgcggacgat
     2041 gccaactggc gaacccagag tgcatggtgg caaagctgcg tggcaacggt aacctacatt
     2101 tactcgacgc tgaatcgcta cgatcccacc tcttttgctg attctcaggc aattattgaa
     2161 ttccgacaaa aattagcttc agaaatcaac aagctgcagc atgccattac cgaaggtcag
     2221 tgctggcaaa gcgactggcg gatcagtgaa agtgaagcga tggcggcacg ggaatgtaac
     2281 ctggagaata tctgccagac gctgttacag cttggtcaga tggacccgaa tacgccgcca
     2341 acgcccgcag ccaaaccgcc atcaatggcc gccgatgctt ttaccaatcc agactatatg
     2401 cgctacgcgg taaaaacgct gctcgcctgt ttgatctgtc acaccttcta cagcggcgtg
     2461 gactgggaag gcattcacac ctgtatgctg acctgcgtga tcgtcgctaa tccgaatgtc
     2521 ggttcatcgt accagaagat ggtgctgcgt tttggcgggg ccttttgcgg cgcgattctg
     2581 gcgctgttat tcacgctact ggtcatgccc tggctggaca atattgtcga attgctgttt
     2641 gtgctggcac cgattttcct gttgggcgca tggattgcca ccggctctga acgctcttct
     2701 tatatcggca cacagatggt ggtcaccttc gcgctcgcca cgctcgaaaa cgtttttggc
     2761 ccggtgtacg acctggtgga aattcgcgat cgcgccctgg gtatcatcat tggtaccgtg
     2821 gtgtccgcgg tgatttacac ctttgtctgg cctgaaagtg aagcgcgcac gctgccgcaa
     2881 aaactgactg tcgcgctggg tatgttaagt aaagtaatgc ggatcccacg ccagcaggaa
     2941 gtcacggctc tgcgcactta tctgcaaatt cgtataggtc tgcatgcggc gtttaatgcc
     3001 tgtgaagaga tgtgccaacg cgtggcgctg gagcgtcaac tggacagcga agaacgcgcc
     3061 ttactgattg aacgttcgca aacggttatt cgtcagggcc gcgatcttct tcacgcctgg
     3121 gatgccacct ggaactcggc gcaggcgctg gataacgcac tacagccgga cagagcaggt
     3181 cagtttgccg acgccctgga aaaatacgct gccggtctgg caaccgcact cagccgttct
     3241 cctcaaataa cgcttgaaga gacacccgcc tctcaggcca tcctgcccac cttattaaaa
     3301 caggagcaac acgtctgcca gcttttcgcc cgcttgccag actggacagc cccggcatta
     3361 acacccgcca cggaacaggt acaaggagcc acgcaatgat caatcgtcaa ctttcacgtc
     3421 tgctgttgtg cagcattctc ggcagcacga cgctgatttc cggctgtgcc ctggtacgta
     3481 aggattctgc gcctcatcaa cagctcaaac cggaacaaat caaactggcc gatgatattc
     3541 atcttgccag ctccggctgg ccgcaggcgc agtggtggaa acaactcaat gacccgcagt
     3601 tggatgccct gatccagcgg acgctaagcg gttcacacac cctcgcagaa gcgaaactgc
     3661 gggaagaaaa agcgcagtcc caggccgatt tgttagatgc cggttcacag ttgcaggtgg
     3721 cagcgttagg gatgctcaac cgccaacgtg tctcggcgaa cggcttttta agcccttatg
     3781 cgatggatgc gccagcactg gggatggacg ggccgtacta tacggaagcc acagtaggtt
     3841 tgtttgccgg actggatctt gatttgtggg gtgtgcatcg ctcagcggtt gccgccgcca
     3901 ttggcgcgca taatgccgcg ctggcagaaa ccgcagcagt agagctatcg ctgaccacgg
     3961 gcgtagcgca gctttattac agtatgcagg ccagctatca gatgctcgat ctgttagaac
     4021 aaactcgcga tgtgattgat tacgcggtga aagcgcacca aagtaaagtg gcacacggtc
     4081 tggaagcgca agtgcctttc cacggcgcgc gggcacagat tctggcggtc gataaacaaa
     4141 ttgctgccgt taaagggcaa atcaccgaaa cgcgagaatc tctgcgcgca ttgattggcg
     4201 cgggagccag cgatatgccg gagatcaaac cggtggcatt accgcaagtc cagactggca
     4261 ttccggcgac gctctcttat gagttgctcg ccagacgccc ggatctgcaa gccatgcgct
     4321 ggtatgttca ggcgtcatta gatcaggtgg attccgcgcg ggcgctgttc tacccaagct
     4381 ttgatatcaa agcgtttttc ggccttgatg ccatccacct ggatacctta ttcaaaaaaa
     4441 acagtcgcca gttcaacttc atcccgggtc tgaaattgcc gctgtttgac ggtggacggt
     4501 tgaatgccaa tctcgaaggc actcgcgccg ccagcaacat gatgattgaa cgttacaacc
     4561 agtcagtact gaacgcggtg cgtgacgttg ccgtcaacgg cacgcgtctg caaacgctca
     4621 acgacgagcg agaaatgcag gctgaacgcg tggaagcaac gcgcttcacc cagcgcgctg
     4681 ccgaggccgc ctatcagcgc ggcttaacca gccgcttaca ggccactgaa gcccggttgc
     4741 cagtacttgc cgaagagatg tcattactga tgctggacag ccgccgggtg ctccaaagca
     4801 ttcagttgat gaaatcgctg ggcggcgggt atcaggcagg tcccgtcgtc gagaaaaaat
     4861 aaaatgtctg ccgcgtgatg gctgtcacgc ggtatttcgt ttcgtcacgt caaaactgac
     4921 gacagcctgt ttttcgtcag agttttgaat aaatagtgcc cgtaatatca gggaatgacc
     4981 ccaaataaaa tgtggcataa aagatgcata ctgtagtcga gagcgcgtat gcgtgatttg
     5041 attaactgga gcgagaccga tgaaaaaagt cgtcacggtt tgcccctatt gcgcatcagg
     5101 ttgcaaaatc aacctggtcg tcgataacgg caaaatcgtc cgggcggagg cagcgcaggg
     5161 gaaaaccaac cagggtaccc tgtgtctgaa gggttattat ggctgggatt tcattaacga
     5221 tacccagatc ctgaccccgc gcctgaaaac ccccatgatc cgtcgccagc gtggcggcaa
     5281 actcgaacct gtttcctggg atgaggcact gaattacgtt gccgagcgcc tgagcgccat
     5341 caaagagaag tacggtccgg atgccatcca gacgaccggc tcctcgcgtg gtacgggtaa
     5401 cgaaaccaac tatgtaatgc aaaaatttgc gcgcgccgtt attggtacca ataacgttga
     5461 ctgctgcgct cgtgtctgac acggcccatc ggttgcaggt ctgcaccaat cggtcggtaa
     5521 tggcgcaatg agcaatgcta ttaacgaaat tgataatacc gatttagtgt tcgttttcgg
     5581 gtacaacccg gcggattccc acccaatcgt ggcgaatcac gtaattaacg ctaaacgtaa
     5641 cggggcgaaa attatcgtct gcgatccgcg caaaattgaa accgcgcgca ttgctgacat
     5701 gcacattgca ctgaaaaacg gctcgaacat cgcgctgttg aatgcgatgg gccatgtcat
     5761 tattgaagaa aatctgtacg acaaagcgtt cgtcgcttca cgtacagaag gctttgaaga
     5821 gtatcgtaaa atcgttgaag gctacacgcc ggagtcggtt gaagatatca ccggcgtcag
     5881 cgccagtgag attcgtcagg cggcacggat gtatgcccag gcgaaaagcg ccgccatcct
     5941 gtggggcatg ggtgtaaccc agttctacca gggcgtggaa accgtgcgtt ctctgaccag
     6001 cctcgcgatg ctgaccggta acctcggtaa gccgcatgcg ggtgtaaacc cggttcgtgg
     6061 tcagaacaac gtgcagggtg cctgcgatat gggcgcgctg ccggatacgt atccgggata
     6121 ccagtacgtg aaagatccgg ctaaccgcga gaaattcgcc aaagcctggg gtgtggaaag
     6181 cctgccagcg cataccggct atcgcatcag cgagctgccg caccgcgcag cgcatggcga
     6241 agtgcgtgcc gcgtacatta tgggcgaaga tccgctgcaa actgacgcgg agctgtcggc
     6301 agtacgtaaa gcctttgaag atctggaact ggttatcgtt caggacatct ttatgaccaa
     6361 aaccgcgtcg gcggcggatg ttattttacc gtcaacgtcg tggggcgagc atgaaggcgt
     6421 gtttactgcg gctgaccgtg gcttccagcg tttcttcaag gcggttgaac cgaaatggga
     6481 tctgaaaacg gactggcaaa tcatcagtga aatcgccacc cgtatgggtt atccgatgca
     6541 ctacaacaac acccaggaga tctgggatga gttgcgtcat ctgtgcccgg atttctacgg
     6601 tgcgacttac gagaaaatgg gcgaactggg cttcattcag tggccttgcc gcgatacttc
     6661 agatgccgat caggggactt cttatctgtt taaagagaag tttgataccc cgaacggtct
     6721 ggcgcagttc ttcacctgcg actgggtagc gccaatcgac aaactcaccg acgagtaccc
     6781 gatggtactg tcaacggtgc gtgaagttgg tcactactct tgccgttcga tgaccggtaa
     6841 ctgtgcggca ctggcggcgc tggctgatga acctggctac gcacaaatca ataccgaaga
     6901 cgccaaacgt ctgggtattg aagatgaggc attggtttgg gtgcactcgc gtaaaggcaa
     6961 aattatcacc cgtgcgcagg tcagcgatcg tccgaacaaa ggggcgattt acatgaccta
     7021 ccagtggtgg attggtgcct gtaacgagct ggttaccgaa aacttaagcc cgattacgaa
     7081 aacgccggaa tacaaatact gcgccgtgcg cgtcgagccg atcgccgatc agcgcgccgc
     7141 cgagcagtac gtgattgacg agtacaacaa gttgaaaact cgcctgcgcg aagcggcact
     7201 ggcgtaatac agtcctttct acagcctcct ttcggaggct gtttttttat ccattcgaac
     7261 tctttatact ggttactccc tacccaatcg tattatcaaa atgaaaaaaa ttatcgcatt
     7321 gatgttgttt ttgacattct ttgcccacgc gaacgactcc gagcctggca gccagtattt
     7381 aaaggcagca gaggccgggg accgacgcgc acaatatttt cttgccgaca gctggtttag
     7441 ctccggcgat ttgagcaaag ccgaatattg ggcacagaaa gccgccgaca gcggtgatgc
     7501 tgatgcctgc gcgctgctgg cgcagatcaa aatcaccaat ccggtcagtc tggactatcc
     7561 acaagcaaaa gttcttgcag agaaagcggc gcaagcgggc agtaaagaag gtgaagtaac
     7621 gctggcgcat attctggtaa atactcaagc gggcaaaccg gattatccaa aggcgatttc
     7681 gctgttagaa aacgcctcgg aagatctgga gaacgactct gccgtcgatg cccaaatgct
     7741 gcttggtttg atttacgcca acggcgtggg cattaaggcc gacgatgaca aggcaacctg
     7801 gtatttcaaa cgcagctctg caatttcccg aaccggttat tccgagtact gggcgggaat
     7861 gatgttctta aacggtgaag aaggttttat cgagaagaac aagcaaaagg cgctgcactg
     7921 gttgaacctg agctgtatgg aagggtttga taccgggtgt gaagagtttg aaaaattaac
     7981 caacggttaa ggtaggcctg ataaggcgcg ttaagcgtcg catcaggcgt tgatgtcgga
     8041 tgcggcgtaa acgccttatc cgacctacaa aatcacgcta aatcaggcta ttgcgctgtc
     8101 tggcgtaggc ctgataagac gcggcaagcg tcgcatcagg cattgatgtc ggatgcggcg
     8161 taaacgcctt atccgaccta caaaatcacg ctaaatcagg ctattgcgct gtctggcgta
     8221 ggcctgataa gacgcggcaa gcgtcgcatc aggcattcac acattgccgg ggggatatcc
     8281 ccggcaatct tcaattattg atccgcagtt ttatcaaatt tgcccagcac ttcacgctca
     8341 taagccagcg cctttttgcg gtcaaatttg tgttcccact tggcaatcac cagcaccgcc
     8401 agcgcattac ccaccacgtt cagcgcagta cgcgccatgt cgaggatacg gtcaacacca
     8461 gcaataaacg ccagaccttc cagcggaata cctacgctac ccagcgttgc cagcaacacc
     8521 acaaacgaca cgcccggcac gccagcaatc cctttcgagg tcaccatcag cgtcagcacc
     8581 agaatgattt cctgccagat ggacaggtca atgccataca gctgagcgat gaaaatagcg
     8641 gcaatacttt gatacagcgt cgaaccatca aggttaaaag agtaaccggt cggcaccacg
     8701 aagctggtga tcgacgccgg tgctccgtag gcttccatct tctcaataat tcgcggcagt
     8761 acgctttcag agctggcagt ggagtacgcc agaatcagct catctttcag aatacgaatc
     8821 aggatccaga cgcttaaccc gcacaggcgc gccacaattc ccagcactac cagcgcgaag
     8881 aacagaatgg cgaaatgcac cagcagcacc agtttcgcca gtggccacag agaagagaaa
     8941 ccaaagttag ccaccgtcac cgcaatcagc gcaaacacac ccaccggcgc ataacgcatc
     9001 accatgtgag tcactttaaa catggtttca gagatggagc ggaacacggt caccagcggt
     9061 tcacgatgcg tcgcgggcag ggaggaaagc cccagaccaa acagcaccga gaagaagatg
     9121 atcggcagca tttcgccttt cgccatcgac gccacaatat tcgtcggcac cagcgacaaa
     9181 atcgtgccca taatgccgtg ggaactgctt tgtaccgctt ccgtagtgct ctgatatttc
     9241 gagatatcga cggtcgccaa ctgcgacata tccaccccgg caccgggctg gaagacgttc
     9301 gccagagtga tccccaaaat gatggcgacg gtggtgatca cctcgaagta gataatggtt
     9361 ttcgcgccaa tacgcccgag ctgtttggca tcaccaacac ccgcgatacc caccaccagc
     9421 gtggagatca caatcggcac aacaatcatt ttgatcagat ggatgaagat atcacccgcc
     9481 ggagagagca aattgacgac cagccagtcg cggctgtcgc tatggtagtg caggtagctt
     9541 cccaggagaa tgcccagcac catagcaaac agaatctgcc aggccaggct gaattttata
     9601 tttttcatca gaataacttc ctcaatggag cgccgttccc cgttcagaaa cgcgagaacg
     9661 tgacatactg ccagggcatg aattgtgttg cgttggtttg tagatttttt taacgcttat
     9721 gatcagtatc atttgcgcgg tatgttgcgc aaccctttaa caacagagct tttcgctgca
     9781 ttaatgcata ataacgcgag tttatggcag gattaatgaa taaccatttg ttatggaaag
     9841 atttttattt gtttaatctg caataatact gattggtgat tatttttggt atatgttcat
     9901 tcgattattt tttatgcagc tcaataacat aatcttcatc accagatgat taatccgatc
     9961 tccgctgcaa caatttcgcc atattaatcg actcgaccag ctgtgtccgg ttgatccggg
    10021 gtgagttgag atccatcact ttttgccata gttcgatggc ttgcgcgtaa ttcgcctgca
    10081 taaacgcatc ggaagccagc agcatcagag cggtgatttc gttagagtcc agcgcgaggg
    10141 ctttgtcgat cattgcgcga gtctgggcgg tcatatgctg gctggcctgg taataaagca
    10201 ccgtcgccag cgccgcatac agttcagcgt tctcaccacg tagttgcaac gcctgacggt
    10261 acgccagcag cgaattgctg taatcgtttt gccacagata gtactcgccc agtaacgccc
    10321 actgttcgct gttttgcgga ttagcgcgga ttttatcctg caatgcctga agctgcgctt
    10381 ccggggtttg ctggctggca aactgatgta gcggatcgcg ctgacgctga tactccgcac
    10441 gtaccgcctg ccattttggg cttaacagat aactgccgac acagaggaat accatcaata
    10501 tcgtgagtgt tgtcagcatt ttcaccggta tttgaggctg tttcatcgct tcgccctcac
    10561 tcgccagagg atcagtgcca tcagcagtaa caacaccact ggcagcgccc acaacaccag
    10621 cgtctgaccc gttaacggtg ggttatagcg aacaaaatct ccgtagcgtt cggtcatcca
    10681 gccaatgatt tcgacttcac ttttcccctc cgccaccatg ctgtaaacct gatggcgcat
    10741 actgactgcc accggcgcgt tggattccag taagttttga ttctggcact gcggacaacg
    10801 taactggctg gcaatattta acgcctgttg ctgttgttgc ggattggcga attgccaggt
    10861 gtctacaacc tgagcgcgcg caaaacaggt aaataacagc agtagcgtga gaagtccttt
    10921 attcatcgcg cttcctcccc cgccatccgc ttaacaatgc gcccgcaatc atcaacaatc
    10981 ctccccccca gatccagcgc acaccgcttt gtacatacaa acgaaaagcg taacgatccg
    11041 gcccagtttt ctcccccatg accgcatacc agtcatggat gccgttccag cgaattgacg
    11101 gttccatcat ttgctgacgg cgtgcttcat aaaaacgccg ctccggcgtc agttcaccaa
    11161 tacgttgctg atggtcaaac agtgccacta tcgctttttc gctggtgtaa ttgcctctgg
    11221 cttgcagatc gaggcgctca aaacggaagg tgtatcctgc cagcgtcacc tgctgacccg
    11281 gctgtaaatt caggctgatt tcctgacggc tgacgctgga aaccacgacc ccagcggcaa
    11341 ataacagcac gcccgcatga gctaccagcg ccggaagctg cacgcgtttg ccagagacaa
    11401 acgtcgccag cacaatcacc accaacatca acagaccaaa cggtaacgtc gcgcggttaa
    11461 aatacggtgc gccaacggag aggcgtcccc agcccagcag gccgtaaatc atcggataaa
    11521 gcgttcccac cagcacgatc agtagcactg cgcaaaacag caacagcgta gcgaggatta
    11581 acatttcccg cgataacccc gaaaaacgca ccgccgggcc accgtcccgc gctcgccagc
    11641 catacagagc cagagacgca aggctaatca gtacaaacag actgaacaac ggcacggcgc
    11701 ggacgttatc cagcgcgaac gcatgaaccg aaaccagaat gccagaacgg acaattaagg
    11761 tgcccagcag cgacagcatc agagtaacta tcgccagtaa cagcgaccag tggcgaaaaa
    11821 tcccccgctg gcgtgtcagg gataaactgt gcagcaacgc agtggcagaa agccagggta
    11881 gtaaagaggc gttttccacc ggatcccaga accaccagcc gccccagccc agttcgcaat
    11941 aggcccacca ggaaccgagg atgatccccg cagttaatgc actccagcca ggtaacgccc
    12001 agcgccagca aattcgggcg caggcaccat caaactcgcc gcgcagtaga ctcgccagcg
    12061 ccacgctcgc cgctaccatc aaaccgccat agccaaggta aagcagcggt ggatgaaaga
    12121 taagaccggg atgttgcagc atcggattga gatcgcggcc ttcgattgct ggtggaaata
    12181 tccgcacgaa gggatcggac cacagcacta caaacagtag cagtgcggcg agcatgagag
    12241 ataaaacggc taacgtcagc ggaaatagcg gatcggtttg ctgccgataa tgccaggcaa
    12301 acagcgcgct ccaggcggaa agcagcagca cccaaagcag cagcgaacct tcatgaccgc
    12361 cccacaccgc cgccagtttg agttcccacg acagcaggct gtagctatgt tgggcgacat
    12421 aaatgaccga gaaatcgctg atgagaaaac aatacgtcag tacgccaaag gcgagcagca
    12481 ggagcgcgaa ctgtgccaga atgccgatgc aagtgagtcg catcatggca ggccagcgca
    12541 accgcactcc cgcaaaggcc gtcaacgggg tcaacacgtt gaccccgaga cttaacaaca
    12601 gcgccagaaa accgacttca ggaaggaata cgtccagaaa ccacctctgt tctggcttac
    12661 gccaccgtta actgcccggc gtagagaatg aaaaagcgca acatcaacac gccgaccagg
    12721 ctggctccac acgccgccag cacggcagga atgccggaac tgcgattgac ccacggtttg
    12781 agcaacattg gcacaatcag ccccagcccg gcgacaccaa gccagaacca ccacgtccag
    12841 aaaccgccac ccaatgccgc caccagcgca cgcactttac cgtcatcgcc caaggccaga
    12901 ccgacaaaaa acgccaccag caggaagatt tcaccccata ccacggggat ttccatacgg
    12961 tgtacaaact gcgcttccgt ggaatgcggg ttactgcgtt ggcgtatcgc catggcgatc
    13021 agtgccaccg ccgcaccgga cgagatgccg gagaagagga acagcaccgg caggatcggg
    13081 ttattaagga acggatacga tttcagcgcc gacagcagaa aaccggtata agcccccaac
    13141 aacaccgcca gcaccagcat caatgtttcc agtccgcgat gaacgggcgt cagcaggctc
    13201 agaacctttt gcacgatccc cagcttcggc aaccagcgtt gttgcagggc aagcaaatca
    13261 tgttcaaaga ttttcgccag ccacagcacc agcaccacca tgtaaagctg aaacagcatc
    13321 acccccatcg acatcaccga ggtaaaactg tagtggaaca tcagcttcca gaaggtccac
    13381 ggtcttgtca ggtggaagac gaggatcaac agaccgagga tcaccgcgcc aggtccgaca
    13441 atcagcgtgg tgcgcagcaa cgtgctgtct gcaccgcccg cctgcgggta gaagcgacgt
    13501 aacagcacag ccagcgtcac caaaccagca gaaataccaa tcaaaaacag gtagatggca
    13561 atcggccagt cccacaccag cgattcaaaa tgaaatgcgg aagtctgcgt cattggctca
    13621 cctccccgta tttaaacggt acgcggtaca gtttcggttt ggttcccagc gccagcttgt
    13681 agcggtaagt aggcttctgg cgcagcagtt gcgaaatctc actgttagga tcgtccagat
    13741 tgccaaacgt cagcgccttc gtcgggcaag cctcaacgca cgcgggcagc ttaccggcct
    13801 gcaaattggt cttacggcag aaatcgcatt tgtccgccgt cttcgtgacc ggatggataa
    13861 agcgcacgcg gtacggacag gcggcgatgc agtactgaca accgacgcag agatcaggat
    13921 taacatcgac aatcccgcta gcggcatcac gaaaagacgc accggtcggg cagacgtcaa
    13981 cgcacggcgc atgatcgcag tgctggcaag acttacggaa gaagcgatat ttcacatcag
    14041 gaaattcgcc ctgcggctcg ctacgaataa tcgtcaggcg cgagacgcct tccggcacct
    14101 tgttcacttc ccgacaagca tccatacagg cggtacagcc gatgcataac gactcgtcat
    14161 gcaccatgcc gtaacgcacc ccattgatat tcaacgtttt cgccaccacg cgccctgcgg
    14221 tgccgctgac ggctgccagc acgccgacgc cggtgagaaa ctggcgacga gaccaggtca
    14281 tggctgctcc ttaagcaacg gaacagacgc cgggttaaag ttcggattgg tgcgctgatc
    14341 gctgtggcaa tcgacgcaaa tcttaatccg tcctttgtca cttaacgtct gcatcgtatc
    14401 ttgctgtgga tgcagggaat ggcagctggc gcacgccact ttggttacgt ggacatcgtg
    14461 cggccagaac gctttttgca actgttccgg cagatggcag gacatacaga cgctgttctg
    14521 ctctccaacc ttgtacatcg gctcgttaaa gcgcatcaca tctttcaccc cttcgcggtg
    14581 ttgcggtgat ggctggccgt gacagttggt gcaggtgacc ggcagtttgt tattcgggtt
    14641 gataacggaa gcatgtttgc catgcatacc ttcggtatct ggcttatgac agtccagaca
    14701 ggcggcatcc gggttacgct gctgggtaac ttcgtagcga tcatcagatg cctgcgccgc
    14761 aggggtagcg gtaattccgt tcaggctcca caacaggccc gacgccagca ccccggcagt
    14821 taataacgaa cgtaatacgc tcatattcac tccattgtgt tttccgcccc ttgcgaggcg
    14881 gaacggggtt attggcttaa cagaccgttt ttacgtgcct gctcttccca ctgcgggatc
    14941 accgttttga tgaagtcctg cttctcggcc ttgatttgtt ccatgttcag gccaatggcc
    15001 tgctgggctt tctctttggt cgagatatcc gggatctgga tttcatgggt gatgcctttg
    15061 gtcgccagca ggcgcgccag tttggtgcgt gcatccgccg ctttatccat cgccgtaccg
    15121 agcatccgta aaccttcttc cggtgcgtgc atatgaatgc cgtgggaagc gatcgccaga
    15181 tcccagcgcc actgggcatg acggatatcg tcctgaattg gcttcatttc ggcttccgtc
    15241 gcgcctgcat ccagcgctgc tttcgcttcg aagtgagcgt gaaccagttg atcttcaacc
    15301 ttgattttca ggtcgttaat cgactgctta cgttccgcga ccactttttg caaggcagct
    15361 ttgtcctggg tatggcagtt cgcacaagtc tgggcgaagt tatcaaacgg attaccaatt
    15421 ttatggtcgg tgtagagttt gccttcggcg ttctgcactt ttggcatatg gcagtcgata
    15481 caggtcacgt tgtttttacc gtgaatgccc gctgtccagg tttcatattc cgggtgctgc
    15541 gctttcagca ttggcgtttt cgacagggag ttagtccagt cagagaaggc aattttgtcg
    15601 taatactgct ccatattttc gactttcatg ccgtcatccc acgggaattt aaccgctttg
    15661 tttttgccgt cgaagtaata ctccacatgg cactgaccgc aaaccatcga ttgctggtcg
    15721 aaacgtccgg ctttctcaaa aggtttacca atggcttcca tcgcgcgagc cgcatacgga
    15781 cgggaaaggg ttaactccgg tttgcctttg gcgaactctg gtgaggcggt gttatggcaa
    15841 tcggcacaac ctaagttgtt gacaatttcc gggccgccgc gcgcccattt accgtggaag
    15901 tagccatctt cgccgtcttt ctggatcaga cgcgccacat ccgggctttt acaactccag
    15961 cacgccatcg gtagcggacc atcttcagcg tttttcggcg caccggtacg cagggtttca
    16021 cgcacatcgg tcacagcaaa agcatgtcca cgcggcttgt tgtaatcgcg cgagaaggga
    16081 taccccgccc acaggatcac cagccgtgga tcttccgcca gggcgtcaac acgctctgac
    16141 tgttccgagg tggctttcca ggagagatat tgatcgggat gctgcggggc aaaggtttca
    16201 ttcttcgctt ccacagttac aggttttgcg ggagcagccg tttgttcagc gtgaacagaa
    16261 gtgaaaaaga aaaaaggaat caataagctg aagatacggc gtgcgtttat ttttatcctt
    16321 gtcatagggg cttcatccga attgcgccat tgttgcaatg gcggttttta ttgtttttca
    16381 cgacagtaac cgcacctaca ctgtcatgac attgctcgcc cctatgtgta acaaataacc
    16441 acactgtgaa tgttgtcttt aatcaattgt aagtgcatgt aaaataccac tttagagtta
    16501 gtcagtatct tcctcttttt caacagcatg cataactgca tgttcctcaa agaattaatc
    16561 aacttttgtt gctgaccttc aaaaattacc ctgccgttta tttgcacaat tctacttttg
    16621 cgtgatctgt cgcccaaata ctaaacaaaa ctgccaatac ccctacattt aacgcttatg
    16681 ccacatatta ttaacatcct acaaggagaa caaaagcatg agtcaaattc acaaacacac
    16741 cattcctgcc aacatcgcag accgttgcct gataaaccct cagcagtacg aggcgatgta
    16801 tcaacaatct attaacgcac ctgatacctt ctggggcgaa cagggaaaaa ttctcgactg
    16861 gatcaaaccg taccagaagg tgaaaaacac ctcctttgcc cccggtaatg tgtccattaa
    16921 atggtacgag gacggcacgc tgaatctggc ggcaaactgc cttgaccgcc atctgcaaga
    16981 aaacggcgat cgtaccgcca tcatctggga aggcgacgac gccagccaga gcaaacatat
    17041 cagctataaa gagctgcacc gcgacgtctg ccgcttcgct aataccctgc tcgagctggg
    17101 cattaaaaaa ggtgatgtgg tggcgattta tatgccgatg gtgccggaag ccgcggttgc
    17161 gatgctggcc tgcgcccgta ttggcgcggt gcattcggta attttcggtg gcttctcgcc
    17221 ggaagcggtt gccgggcgca ttatcgattc caactcacga ctggtgatca cttccgacga
    17281 aggcgtgcgc gccgggcgta gtattccgct gaagaaaaac gttgatgacg cactaaaaaa
    17341 cccgaacgtc accagcgtag agcatgtggt ggtactgaag cgtactggcg gaaaaattga
    17401 ctggcaggaa gggcgcgacc tgtggtggca cgacctggtt gagcaagcca gcgatcagca
    17461 ccaggcggaa gagatgaacg ccgaagatcc gctgtttatt ctctatacct ccggttctac
    17521 cggaaaacca aaaggcgtac tgcacactac cggcggttat ctggtgtacg cggcgctgac
    17581 ctttaaatat gtctttgatt atcatccggg cgatatctac tggtgcaccg ccgatgtggg
    17641 ttgggttacc ggacacagtt atttgctgta cggcccgctg gcctgcggcg cgaccacgct
    17701 gatgtttgaa ggcgtaccga actggccgac gcctgcccgt atggcacagg tggtggacaa
    17761 gcatcaggtc aatattctct ataccgcgcc cacggcgatt cgcgcgctga tggcggaagg
    17821 cgataaagcg atcgaaggca ccgaccgttc gtcgctgcgc attctcggtt ccgtgggcga
    17881 gccaattaac ccggaagcgt gggagtggta ctggaaaaaa atcggcaacg agaaatgtcc
    17941 ggtggtcgat acctggtggc agaccgaaac cggcggtttc atgatcaccc cgctgcctgg
    18001 cgctaccgag ctgaaagccg gttcggcaac acgtccgttc ttcggcgtgc aaccggcgct
    18061 ggtcgataac gaaggtaacc cgctggaagg ggccaccgaa ggcagtctgg tgatcaccga
    18121 ctcctggccg ggtcaggcgc gtacgctgtt tggcgatcac gaacgttttg agcagaccta
    18181 tttttccacc ttcaaaaata tgtatttcag cggcgacggc gcgcgtcgcg atgaagatgg
    18241 ctattactgg ataaccgggc gcgtggacga cgtgctgaac gtctctggtc accgtctggg
    18301 gacggcggag atagagtcgg cgctggtggc acatccgaag attgccgaag ccgccgtagt
    18361 aggtattccg cacaatatta aaggtcaggc gatctacgcc tacgtcacgc ttaatcacgg
    18421 ggaggaaccg tcaccagaac tgtacgcaga agtccgcaac tgggtgcgta aagagattgg
    18481 cccgctggcg acgccagacg tgctgcactg gaccgactcc ctgcctaaaa cccgctccgg
    18541 caaaattatg cgccgtattc tgcgcaaaat tgcggcgggc gataccagca acctgggcga
    18601 tacctcgacg cttgccgatc ctggcgtagt cgagaagctg cttgaagaga agcaggctat
    18661 cgcgatgcca tcgtaaccca caattgccgg atgcggcgta taacgcctta tccggcctac
    18721 attcggcaag ggttacccga gcgttaacct tctcccataa gggagcggga attaaaacaa
    18781 tccctacatt acctctggag aatctgtgat gaatggtacg atttatcagc ggatagaaga
    18841 caatgcgcat ttcagggagt tagtcgaaaa acggcaacgg tttgccacca tcctgtcgat
    18901 tattatgctg gcagtttata tcggctttat tttactgatc gccttcgcgc ccggctggct
    18961 gggcaccccg ctgaatccga acaccagcgt cacacgcggt attccagtcg gtgttggggt
    19021 aatagtgatc tcctttgttc tcactggtat ctacatctgg cgggcgaacg gcgaattcga
    19081 ccgtcttaat aacgaagtcc tgcatgaggt acaagcatca tgaaaagagt tctgacggcg
    19141 cttgccgcca cactcccttt cgcagctaac gccgcggatg ctattagcgg ggccgtagag
    19201 cgccagccaa cgaactggca ggcgattatt atgttcctga ttttcgtcgt gtttacgctc
    19261 ggcattacct actgggcgtc aaaacgcgta cgttcgcgta atgactatta caccgcaggc
    19321 ggcaatatca ccggttttca gaacgggctg gcgattgccg gtgactatat gtccgccgcc
    19381 tcatttttgg ggatctccgc actggtgttt acctccggct atgacggctt aatttactcg
    19441 ctgggcttcc tggtgggctg gccgatcatt ttgttcctga ttgccgaacg tctgcgtaac
    19501 ctggggcgtt acacctttgc cgatgtagcc tcttatcgtc tgaaacaagg gccgattcgt
    19561 attctttcgg cctgtggttc tctggtggtg gtggcgcttt accttatcgc ccagatggtg
    19621 ggcgcaggta aactgatcga gctgctgttt ggccttaact atcacattgc agtggtgctg
    19681 gtcggcgtgc tgatgatgat gtacgtcctg ttcggcggca tgctggcgac cacttgggta
    19741 caaattatca aagctgtgct gttgctgttc ggtgccagtt ttatggcctt tatggtgatg
    19801 aaacacgtcg gctttagctt caacaatctg ttcagcgaag cgatggcggt acacccgaaa
    19861 ggtgtcgaca tcatgaagcc gggagggctg gtgaaagatc cgatctccgc gctgtcgctg
    19921 gggcttggat tgatgtttgg tacggctggc ttgccgcaca ttctgatgcg cttctttacg
    19981 gtcagcgatg cccgcgaagc gcgtaagagc gtgttctacg ctaccggatt tatgggctac
    20041 ttctatatcc tgacctttat tatcggcttc ggcgcgatca tgctggtcgg tgcaaatccg
    20101 gaatataaag acgcggcggg ccatctgatt ggtggtaaca acatggcggc cgttcacctg
    20161 gcgaatgcag tgggcggcaa cctgttcctc ggttttattt cagcggttgc tttcgccact
    20221 atcctcgcgg tggttgcggg tctgacgctg gcgggcgcat cagcggtttc gcatgacctg
    20281 tacgctaacg tcttcaaaaa aggcgcgacc gaacgtgaag agctgcgggt atcgaaaatc
    20341 accgtcctga ttttgggtgt gattgcgatt atcctcggcg tgctgtttga gaatcagaac
    20401 atcgccttta tggtggggct ggcgtttgcc atcgcggcga gctgtaactt cccgatcatt
    20461 ctgctttcta tgtactggtc gaaactgact acgcgtggcg caatgatggg ctgctggctg
    20521 gggctaatta ccgcagtggt actgatgatc ctcggcccga cgatttgggt acagatcctc
    20581 ggtcacgaaa aagccatctt cccgtatgaa tacccggcgc tgttctctat cagcgtggca
    20641 ttcctcggca tctggttctt ctcggcaacc gataactcag cggaaggtgc gcgcgagcgt
    20701 gaactgttcc gtgcgcagtt tatccgctcc cagaccggct ttggcgttga gcaaggccgc
    20761 gcgcattaat ctctcccctt ccccggtcgt ctgaccgggg aatactcttc ctctcccaaa
    20821 tgcatcacct tttcccataa tattaaacaa ataaactcat taaaaaatga gcgatttttg
    20881 acagtcgtag aaaatgataa tgcagagaat atgcctttt
//
LOCUS       DAZKFU020000067        20638 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZKFU020000067 DAZKFU020000000
VERSION     DAZKFU020000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.067"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            183..632
                     /gene="alaE"
                     /locus_tag="ACHOQQ_RS09705"
                     /old_locus_tag="pgaptmp_001941"
     CDS             183..632
                     /gene="alaE"
                     /locus_tag="ACHOQQ_RS09705"
                     /old_locus_tag="pgaptmp_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGU4470199.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKISPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(669..1013)
                     /locus_tag="ACHOQQ_RS09710"
                     /old_locus_tag="pgaptmp_001942"
     CDS             complement(669..1013)
                     /locus_tag="ACHOQQ_RS09710"
                     /old_locus_tag="pgaptmp_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGU4470200.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1165..1494
                     /locus_tag="ACHOQQ_RS09715"
                     /old_locus_tag="pgaptmp_001943"
     CDS             1165..1494
                     /locus_tag="ACHOQQ_RS09715"
                     /old_locus_tag="pgaptmp_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGU4470201.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQSARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1644..2978)
                     /locus_tag="ACHOQQ_RS09720"
                     /old_locus_tag="pgaptmp_001944"
     CDS             complement(1644..2978)
                     /locus_tag="ACHOQQ_RS09720"
                     /old_locus_tag="pgaptmp_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4470202.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGENQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            3066..3497
                     /locus_tag="ACHOQQ_RS09725"
                     /old_locus_tag="pgaptmp_001945"
     CDS             3066..3497
                     /locus_tag="ACHOQQ_RS09725"
                     /old_locus_tag="pgaptmp_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU4470203.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIAKT
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3706..3951
                     /gene="nrdH"
                     /locus_tag="ACHOQQ_RS09730"
                     /old_locus_tag="pgaptmp_001946"
     CDS             3706..3951
                     /gene="nrdH"
                     /locus_tag="ACHOQQ_RS09730"
                     /old_locus_tag="pgaptmp_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGU4470204.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3948..4358
                     /gene="nrdI"
                     /locus_tag="ACHOQQ_RS09735"
                     /old_locus_tag="pgaptmp_001947"
     CDS             3948..4358
                     /gene="nrdI"
                     /locus_tag="ACHOQQ_RS09735"
                     /old_locus_tag="pgaptmp_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGU4470205.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4331..6475
                     /gene="nrdE"
                     /locus_tag="ACHOQQ_RS09740"
                     /old_locus_tag="pgaptmp_001948"
     CDS             4331..6475
                     /gene="nrdE"
                     /locus_tag="ACHOQQ_RS09740"
                     /old_locus_tag="pgaptmp_001948"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4470206.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFDHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VVDERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYNENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6485..7444
                     /gene="nrdF"
                     /locus_tag="ACHOQQ_RS09745"
                     /old_locus_tag="pgaptmp_001949"
     CDS             6485..7444
                     /gene="nrdF"
                     /locus_tag="ACHOQQ_RS09745"
                     /old_locus_tag="pgaptmp_001949"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4470207.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKRLEKISEAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7800..9002
                     /gene="proV"
                     /locus_tag="ACHOQQ_RS09750"
                     /old_locus_tag="pgaptmp_001950"
     CDS             7800..9002
                     /gene="proV"
                     /locus_tag="ACHOQQ_RS09750"
                     /old_locus_tag="pgaptmp_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGU4470208.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKAIDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8995..10059
                     /gene="proW"
                     /locus_tag="ACHOQQ_RS09755"
                     /old_locus_tag="pgaptmp_001951"
     CDS             8995..10059
                     /gene="proW"
                     /locus_tag="ACHOQQ_RS09755"
                     /old_locus_tag="pgaptmp_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGU4470209.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10117..11109
                     /gene="proX"
                     /locus_tag="ACHOQQ_RS09760"
                     /old_locus_tag="pgaptmp_001952"
     CDS             10117..11109
                     /gene="proX"
                     /locus_tag="ACHOQQ_RS09760"
                     /old_locus_tag="pgaptmp_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGU4470210.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11301..12485
                     /locus_tag="ACHOQQ_RS09765"
                     /old_locus_tag="pgaptmp_001953"
     CDS             11301..12485
                     /locus_tag="ACHOQQ_RS09765"
                     /old_locus_tag="pgaptmp_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4470211.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYNDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12609..13346
                     /gene="ygaZ"
                     /locus_tag="ACHOQQ_RS09770"
                     /old_locus_tag="pgaptmp_001954"
     CDS             12609..13346
                     /gene="ygaZ"
                     /locus_tag="ACHOQQ_RS09770"
                     /old_locus_tag="pgaptmp_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGU4470212.1"
                     /translation="MESPTTQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13336..13671
                     /gene="ygaH"
                     /locus_tag="ACHOQQ_RS09775"
                     /old_locus_tag="pgaptmp_001955"
     CDS             13336..13671
                     /gene="ygaH"
                     /locus_tag="ACHOQQ_RS09775"
                     /old_locus_tag="pgaptmp_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGU4470213.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13762..14292
                     /gene="emrR"
                     /locus_tag="ACHOQQ_RS09780"
                     /old_locus_tag="pgaptmp_001956"
     CDS             13762..14292
                     /gene="emrR"
                     /locus_tag="ACHOQQ_RS09780"
                     /old_locus_tag="pgaptmp_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGU4470214.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14419..15591
                     /gene="emrA"
                     /locus_tag="ACHOQQ_RS09785"
                     /old_locus_tag="pgaptmp_001957"
     CDS             14419..15591
                     /gene="emrA"
                     /locus_tag="ACHOQQ_RS09785"
                     /old_locus_tag="pgaptmp_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGU4470215.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQTFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15608..17146
                     /gene="emrB"
                     /locus_tag="ACHOQQ_RS09790"
                     /old_locus_tag="pgaptmp_001958"
     CDS             15608..17146
                     /gene="emrB"
                     /locus_tag="ACHOQQ_RS09790"
                     /old_locus_tag="pgaptmp_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGU4470216.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17210..17725)
                     /gene="luxS"
                     /locus_tag="ACHOQQ_RS09795"
                     /old_locus_tag="pgaptmp_001959"
     CDS             complement(17210..17725)
                     /gene="luxS"
                     /locus_tag="ACHOQQ_RS09795"
                     /old_locus_tag="pgaptmp_001959"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGU4470217.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17875..19431)
                     /gene="gshA"
                     /locus_tag="ACHOQQ_RS09800"
                     /old_locus_tag="pgaptmp_001960"
     CDS             complement(17875..19431)
                     /gene="gshA"
                     /locus_tag="ACHOQQ_RS09800"
                     /old_locus_tag="pgaptmp_001960"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGU4470218.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(19504..19932)
                     /locus_tag="ACHOQQ_RS09805"
                     /old_locus_tag="pgaptmp_001961"
     CDS             complement(19504..19932)
                     /locus_tag="ACHOQQ_RS09805"
                     /old_locus_tag="pgaptmp_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGU4470219.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVATLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19929..20495)
                     /gene="yqaB"
                     /locus_tag="ACHOQQ_RS09810"
                     /old_locus_tag="pgaptmp_001962"
     CDS             complement(19929..20495)
                     /gene="yqaB"
                     /locus_tag="ACHOQQ_RS09810"
                     /old_locus_tag="pgaptmp_001962"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGU4470220.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4925 a         5383 c         5220 g         5110 t
ORIGIN      
        1 aatgtacaaa aaacgagccg ttacggaata ttttatctac aaaaactgac taaataaaaa
       61 tttttcacta attgattagt catagtcagc gatatacgct atgcgaaaat gcagatggca
      121 atgagatcca ctgctttcat ctccattaac atcccattac gcttttatta aggagcatta
      181 gcatgttctc accgcagtca cgcttgcgtc atgcagttgc agatacgttc gcgatggttg
      241 tttactgttc tgtcgtgaac atgtgtattg aagttttcct ctccggaatg agcttcgaac
      301 agtcttttta ttccagattg gtagcgattc cggtgaacat cttaattgca tggccatacg
      361 gtatgtaccg cgatctgttt atgcgcgcgg cacgcaaaat tagcccgtcg ggctggataa
      421 aaaatctggc ggatatcctg gcttatgtga cgttccagtc accggtgtat gtggcgatct
      481 tgttagtggt gggcgcagac tggcatcaga ttatggcggc ggtcagttca aacatcgttg
      541 tttcgatgtt gatgggggcg gtttatggct acttcctcga ttattgccgc cgactgttta
      601 aagtcagcca ttaccagcag gtaaaagcct gactcttctt ttcgcgactg gcatcgccag
      661 tcgcgtcttt aactggcttc gccaaataaa ccattcaaat aacgttcaag cgcaacacgc
      721 gaactaaagc catgcgggat accgtaatgc tcgtgtcgct cgcctgctaa atagagcgga
      781 aattgttgat aatgatcgca agttttaata tcggaagcgg gttcagccag tgtcgaacta
      841 cgttctttta atgttggatg aattaccagt gcggtacgtc ccattcgcgc ttcgcgatta
      901 acatagacat aattttcccc acggcggtaa ccatacgctt tctgggttac cacatcgaca
      961 gtaaaaccga ctttttcaag tacgcgcgcc acctcgtctg gtcgtaaata catatttgat
     1021 cctcgttatt atcaaccgcg ggcctacctt acctgattgc gcattcacaa cgctttcaga
     1081 aaagtccata aagccgcgat gaacctcagt taagaaaatt atggtctaca ctgaaaatta
     1141 catcgaattc taatggggga tcatatgttt aaccgaccga accgcaacga cgttgatgat
     1201 ggcgtgcagg atatccagaa tgatgtcaat caattagctg acagcctcga atctgtattg
     1261 aagtcctggg gcagcgacgc caaaggggaa gctgaagccg cacgcagtaa agctcaggca
     1321 ttgctgaaag aaaccagggc gcgaatgcat ggtcgtactc gcgtccagca atccgcacgc
     1381 gatgccgttg gctgcgcgga ttcttttgtt cgtgaaagac cctggtgtag tgtgggaact
     1441 gctgctgcgg taggtatttt tatcggtgcg ttgttgagca tgcgtaaatc gtagtgagac
     1501 gatgacaagc agtagtctga cttctgaaaa gtactcagtg cgtcaacgcc ttgtccgcct
     1561 acaggcctga aaaacatagc gcatcagggg attttgccct ggtcaccagt cttataggac
     1621 cccgaagcct ggcttcgggg ttattaattt aaagcactgc gcaattgctg tgctacctgt
     1681 cgcgccatac tttcgctaac gatattcgta aacgagagca ataatcctcc gtcctcgctt
     1741 gagcggatac gccagtcgct aagtgcctgt acggcaagac cagcagcatt ggctttatgc
     1801 acgaatgtca cgtcattacc tgctagtttg atcaccatct ggataccgcc tttttgcggc
     1861 acaacctgaa atccctgctg cgtaagtgcc tgctcaatcc actgccgtcg ctgggcataa
     1921 tgctggcgca ttttcttcag atgccgccag aaatgcccct cacggatgaa atctgccagt
     1981 gtgttctgcc acagtacggg tacagcacat ggttccagtg acgcctgctg ccggaattgc
     2041 gaaatctgct ccaccggcac caccagccac gcacagcgca atgcaggaaa tagcgctttg
     2101 ctgaatgttc cggcataaat cacccgctgc ggcgcgtcga gactttttag tgccggtaac
     2161 ggcttaccgt gatagcgaaa ctcactgtcg taatcatctt caataatcca tgcctgacta
     2221 cgctctgccc attccagtat ctgataccta cgcgctaaag agagtgccac acccagcggg
     2281 ctttggtgtg ccggagtaat cagggcaaaa cgcgcatcag gataattttg tattccgctt
     2341 gtgacatcca gtccgttgtc atcaaccggc acaggtaaaa tttccacacc gtggcgagtg
     2401 acaatcgggc gaatcagcgg aaagccggga tcttccatcc acatcccgtc tcccggttgc
     2461 gccagagcgt gcagaattaa agccattgag gccgcataac cgtgagtgat aaagacctgc
     2521 tctggctgac aatcaatccc acgtgaaacg cgtaaataat caacaatagc ctcgcgcaag
     2581 gccgcctcgc cgcagacatc gcctaatgcc aaatcaaaac gcgtctgggt acgcagacga
     2641 cggcccatca cccgcgccca caattcacgc ggaaaaagat caagtgcagg tagccccatc
     2701 tgaaaaggca gcggctgctg attttcacca gcaaaagcca cgacatccac agcagactgt
     2761 tgcggatgta tctgctcact gacaaaggta cccgcctgtc cccgcctgac cagccatccc
     2821 tgcgccacca gctcagcata agcattttcg acagtggatc gagagacacc cagctcctgc
     2881 gaccaggtac ggctcgaggg tagccttgcg ccaggtttca gttctccttg ctcgatagcc
     2941 gtttttaact gacgggcgat atcctgataa cgtggcatat ggtctgcttt tttaggtaat
     3001 ttatggctct ctttagcagt ccattatagt gctacattgc gggcgttaat aagaggagaa
     3061 actgtatgac tacattacgt cagccttact acgaacttag cccggcagtg tataacgcac
     3121 tggtgcaggc caaaacggca ctggaaaata gcacgctgga taccacgttg atggagttaa
     3181 tttatttgcg cgtctcgcaa atcaacggct gcgcattttg tctggagatg cacagcaaag
     3241 cattgcgcaa atccggtgtg ccacaacaca aactggacgc cctggcaggc tggcgcgtaa
     3301 gccatcattt tgatgaacgc gagcgagcgg cgctggcatg ggcggaatca gtgaccgata
     3361 ttgccaaaac ccatgcggaa gacgaggttt atcagccttt gcttgagcat ttcagcgcag
     3421 cagaaatcag cgacttaacg tttgccatcg ggctgatgaa ttgttttaac cgtctggccg
     3481 tttccatgcg gatgtaattt gctgttgccg ggtatgcgcc cggcaaaaaa acacaaaatc
     3541 ccctaccccg ttgagccatt caccggtggc gtaatcaatc taaaattccc atatcttgtg
     3601 tggttgaatc ttttttcaac tacatctagt atctctgtat caacagagag acaacccgac
     3661 gcgtatcatc gcgccgtatc ttcattttaa acggaaatac gaatcatgcg cattactatt
     3721 tacactcgta acgattgcgt tcagtgccac gccaccaaac gggcgatgga aaaccggggc
     3781 tttgattttg aaatgattaa tgtcgatcgc gttcctgaag cggcagaagc gttgcgtgct
     3841 cagggctttc gtcagttgcc ggtagtgatt gctggcgatc ttagctggtc tggtttccgt
     3901 ccggacatga ttaaccgtct gcatccagcg ccacacgcgg ccagtgcatg agccagctcg
     3961 tctacttctc cagcagctcc gaaaacacgc agcgttttat cgaacgttta ggtctgcccg
     4021 cggtgcgcat cccgctcaat gagcgggaac ggattcaggt agacgagcct tacatcctga
     4081 tcgtgccctc ttacggcggc ggcggtacgg ctggcgcggt gccacgacag gtaattcgct
     4141 ttttaaacga cgagcacaac cgggcgttgc ttcgcggcgt tattgcttcg ggtaatcgca
     4201 actttggtga ggcgtatggc cgcgctggag atgtgattgc ccggaaatgc ggcgtgccgt
     4261 ggctgtaccg ctttgaactc atgggtacgc aaagcgatat cgaaaacgtt cgtaaaggag
     4321 taaccgaatt ttggcaacga caaccgcaga atgcctgacg caggaaacga tggattacca
     4381 cgcgctgaat gcgatgctta acctctacga tagcgcaggt cgcattcagt tcgataaaga
     4441 ccgccaggcc gttgacgcct ttattgcgac gcatgtgcgt ccgaacagtg tgaccttcag
     4501 tagccagcag cagcgcctga actggctggt caacgaaggt tactatgatg aaagcgttct
     4561 taatcgctac tctcgcgact ttgtcattac gctgtttgac cacgcacaca ccagcggttt
     4621 tcgtttccag acattcctcg gggcatggaa gttttacacc agctatacgt tgaagacatt
     4681 cgacggtaaa cgttatctgg aagattttgc cgatcgagtc acgatggtgg cgctgacgct
     4741 ggcacaaggc gatgagacgc tggcgttgca actgacagat gaaatgctgt caggacgctt
     4801 tcagccagcc acgccaacat tcctcaactg cggtaagcag cagcgcggcg aactggtttc
     4861 ctgttttttg ctgcgtattg aagacaatat ggagtcgatt ggtcgggcgg taaattccgc
     4921 actgcaactg tcgaaacgcg gcggcggcgt agcatttttg ctgtcgaatc tgcgggaagc
     4981 gggcgcgcca attaaacgta ttgaaaatca atcttctggc gtaattccgg tgatgaaaat
     5041 gctggaagac gcattttcct atgccaacca actcggcgcg cgtcaggggg ctggggcagt
     5101 ctatttacat gctcatcatc ccgatattct gcgttttctc gacacgaaac gggaaaatgc
     5161 cgacgaaaaa atccgcatta aaacactgtc gcttggcgtg gtgatcccgg atatcacttt
     5221 ccatctggca aaagagaatg cgcagatggc gctgttttcg ccttatgacg tagagcgagt
     5281 ttatggcaag ccgtttgccg atgtggccat cagtcaacac tatgacgaac tggttgtcga
     5341 tgaacgcatt cgaaaaaaat acctcaacgc ccgtgatttc ttccagcgac tggcagaaat
     5401 ccagtttgag tccggctatc cgtacatcat gtatgaagac acggtaaacc gtgctaaccc
     5461 tatcgccggg cgcataaata tgagtaatct ctgctcagag attttgcagg ttaacagcgc
     5521 ctcagagtat aacgagaatc tcgactatgc ccgcacaggc catgatatct cctgcaattt
     5581 aggttcgttg aatattgccc acaccatgga ttcccccgac tttgcccgca cggtagagac
     5641 tgccgtgcgc ggtttaacgg cagtatcaga tatgagtcat atccgcagcg tgccgtccat
     5701 cgaagccgga aatgccgcct cgcacgccat cggactgggg cagatgaatt tacacggcta
     5761 tctggcgcga gaaggcatcg cttatggttc gccggaagca ctggatttca ccaatctcta
     5821 tttctatgcc atcacctggc acgcactgcg tacctcgatg ttgctggcac gcgaacgcgg
     5881 tgaaaccttc gccgggttca aacagtcacg ctatgccagc ggtgaatatt ttagccaata
     5941 tctgcaaggt aactggcagc cgaaaaccgc gaaagttggc gaactgtttg cccgcagcgg
     6001 tattacgtta cctacccgtg agatgtgggc gcagctgcgc gatgacgtga tgcgctacgg
     6061 catttacaac cagaatctcc aggcggtgcc gccaaccggt tctatctctt atatcaacca
     6121 tgctacgtcg agtattcatc cgattgtggc gaaagtagag atacgcaaag agggcaaaac
     6181 aggacgcgtt tactaccctg ccccgtttat gactaacgag aatctggcgc tgtatcagga
     6241 cgcttacgaa attggcgcag aaaagatcat cgacacctac gcggaagcga ctcgccatgt
     6301 cgatcagggg ctgtcgctga cgctgttttt ccccgatacc gccaccactc gcgatatcaa
     6361 caaagcgcag atttacgcct ggcgcaaagg tatcaaaacg ctctattaca tccgcctgcg
     6421 tcagatggcg ctggaaggca ctgaaattga aggctgcgtc tcctgtgcac tttaaggaat
     6481 atctatgaaa ctctcacgta tcagcgccat caactggaac aagatatctg acgataaaga
     6541 tctggaggtg tggaatcgcc tgaccagcaa tttctggcta ccagaaaagg tgccactgtc
     6601 gaacgatata cctgcatggc agacattaag tgtcgtagaa caacaactga caatgcgcgt
     6661 ttttactggc ctgacgctgc tcgacacgct gcaaaatgtt atcggcgcgc cttcgctgat
     6721 gcccgatgcg ctcacgcccc atgaagaggc ggtattatcg aatatcagct ttatggaagc
     6781 ggttcatgcc cgttcttaca gttcgatttt ctcgacgttg tgccagacca aagatgtcga
     6841 tgccgcctac gcctggagtg aagaaaacgc accgttgcag cgaaaagctc agattattca
     6901 gcaacattat cgcggtgatg atccgctgaa aaagaaaatc gccagtgtgt ttcttgaatc
     6961 ttttttgttc tattccggtt tctggctgcc gatgtatttt tccagccgcg gaaagctgac
     7021 caataccgcg gacctgatcc gtctgattat ccgcgatgaa gcagtccacg gttactacat
     7081 aggctataaa tatcagaaaa gactggaaaa gatctccgag gcacaacgtg aagagttgaa
     7141 gagtttcgcc ttcgatttgt tgctagaact ctatgacaac gagttgcaat ataccgatga
     7201 gctgtacgcc gaaaccccgt gggctgacga tgtgaaagcg tttctctgtt acaacgccaa
     7261 taaggcgttg atgaatctgg gctacgaacc tttatttccc gcagaaatgg cggaagtgaa
     7321 tccggcaatc ctcgccgcgc tttcgccgaa tgccgatgaa aatcacgatt tcttttccgg
     7381 ttcaggctcc tcttatgtga tggggaaagc ggttgaaaca gaagatgaag actggaattt
     7441 ctgagggtgt tattttcaaa aatatcacta cccgcgacag ggaaataatt cccgccaaat
     7501 agctttttat cacgcaaata ttttgtggtg atctacactg atactctgtt gcattattcg
     7561 cctgaaacca caatattcag gcgttttttc gctatctttg acaaaaaata tcaacttttc
     7621 tcgatttgct ctcagccctt atatcacggg aaattccggc gatttgctca catcaatatt
     7681 catgccacat ttgccctcag gggttgcctc agattctcag tatgttaggg tagaaaaaag
     7741 tgactatttc cattgggtaa tgtatcgaca taaacaaata acaggaatct ttctattgca
     7801 tggcaattaa attagaaatt aaaaatcttt ataaaatatt tggtgagcat ccacagcgag
     7861 ctttcaaata tatcgaacaa ggactttcaa aagaacaaat tctggaaaaa actgggctat
     7921 cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg cgagatattt gtcatcatgg
     7981 gattatccgg ttcgggtaaa tccacaatgg tacgccttct caatcgcctg attgaaccca
     8041 cccgcgggca agtactgatt gatggtgtgg atattgccaa aatatccgac gccgaactcc
     8101 gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc ctttgcctta atgccgcata
     8161 tgaccgtgct ggacaatact gcgtttggta tggaattggc cggaattaat gccgaagaac
     8221 gccgggaaaa agccattgat gcactgcgtc aggtcgggct ggaaaattat gcccacagct
     8281 acccggatga actctctggc gggatgcgtc aacgtgtggg attagcccgc gcgttagcga
     8341 ttaatccgga tattttatta atggatgaag ccttctcggc gctcgatcca ttaattcgca
     8401 ccgagatgca ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc attgtcttta
     8461 tttcccacga tctcgatgaa gccatgcgta ttggcgaccg aattgccatt atgcaaaatg
     8521 gtgaagtggt acaggtcggc acaccggatg aaattctcaa taatccggcg aatgattatg
     8581 tccgtacctt cttccgtggc gttgatatta gtcaggtatt cagtgcgaaa gatattgccc
     8641 gccggacacc gaatggctta attcgtaaaa cccctggctt cggcccacgt tcggcactga
     8701 aattattgca ggatgaagat cgtgaatatg gctacgttat cgaacgcggt aataagtttg
     8761 tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa ggtcttgatg
     8821 cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc gagttgctct
     8881 ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa cagtatgtcg
     8941 gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta aataatggct
     9001 gatcaaaata atccgtggga taccacgcca gcggcggaca gtgccgcgca atccgcagac
     9061 gcctggggta caccggcgac tgcaccgact gacggcggtg gtgctgactg gctgaccagt
     9121 acgcctgcgc caaacgtcga gcattttaat attctcgatc cgttccataa aacgctgatc
     9181 ccgctcgaca gttgggtcac tgaagggatc gactgggtcg ttacccattt ccgtcccgtc
     9241 ttccagggcg tgcgcgttcc ggttgattat atcctcaacg gtttccagca attgctgctg
     9301 ggtatgcccg cgccggtggc gattatcgtt ttcgctctca tcgcctggca gatttccggg
     9361 gtcggaatgg gcgtggcgac gctggtttcg ctgattgcca tcggcgcaat cggtgcctgg
     9421 tcgcaggcaa tggtgactct ggcgctggtg ttaaccgccc tgctgttctg tatcgtcatc
     9481 ggtttgccgt tggggatctg gctggcgaga agtccgcgag cggcgaaaat tattcgtcca
     9541 ctgcttgatg ccatgcagac cacgccagcg tttgtttatc tggtgccaat cgtcatgcta
     9601 tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca tctttgctct gccgccgatt
     9661 atccgtctga ccattctggg gattaaccag gttccggcgg atctgattga agcctcgcgc
     9721 tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc agttaccgct ggcgatgccg
     9781 accattatgg cgggcgttaa ccagacgctg atgctggccc tttctatggt ggtcatcgcc
     9841 tcgatgattg ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg tcgtctggat
     9901 atggggcttg ccaccgttgg cggcgtcggg attgtgatcc tcgccattat cctcgatcgt
     9961 ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca accgtcgctg gtacaccact
    10021 ggccctgtcg gtctgctgac ccgcccattc attaagtaac tctgcacttg cccggtgaca
    10081 ccgggcatat caccctgcca aaaaaaagga ataacaatgc gacatagcgt actttttgcg
    10141 acagcgtttg ccacgcttat ctctacacaa acttttgctg ccgatctgcc gggcaaaggc
    10201 attactgtta atccagttca aagcaccatc accgaagaaa ccttccagac actgctggtc
    10261 agtcgtgcgc tggagaaatt aggttatacc gtcaataaac ccagcgaagt ggattacaac
    10321 gttggctaca cctcgcttgc ttccggcgat gcaaccttca ccgccgtgaa ctggacacca
    10381 ctgcatgaca acatgtacga agctgccggt ggcgataaga aattttatcg tgaaggggta
    10441 tttgttaacg gcgcggcaca gggttacctg atcgataaga aaaccgccga ccagtacaaa
    10501 atcaccaaca tcgcacaact aaaagatccg aagatcgcca aactgttcga taccaacggc
    10561 gacggtaaag cggatttgac tggctgtaac cctggctggg gctgcgaagg tgcgatcaac
    10621 caccagcttg ccgcgtatga actgaccaat accgtgacgc ataatcaggg gaactacgcg
    10681 gcgatgatgg ctgacaccat cagtcgttac aaagagggca aaccggtgtt ttactacacc
    10741 tggacgccgt actgggtgag taatgagctg aagccaggga aagatgtggt ctggttgcag
    10801 gtgccgttct ctgcactgcc gggcgataaa aatgccgata ccaaactgcc gaatggcgcg
    10861 aattacggct tcccggtcag caccatgcat atcgttgcca ataaagcctg ggccgagaaa
    10921 aacccggcag cagcgaaact gtttgccatt atgcagttgc cagtggcaga tattaacgcc
    10981 cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg atattcaggg ccacgttgat
    11041 ggctggatca aagcccacca gcaacagttc gatggctggg tgaatgaggc gttggcagcg
    11101 cagaagtaat ttttattcgg gcggataagg cgtttacgcc gcatccgccg ttctgtacac
    11161 aatgcctgat gcgacgctgg cgcgtcttat catgcctaca aaccacaccg cacatttcaa
    11221 caatctatct ttcatcccat attcatcaac atccgctatt attgatttcc agcttaatca
    11281 tcacctgatg aacaaaaata atgaccaaac ctaatcatga gcttagcccg gcgctaatcg
    11341 tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa ctattacgcc cagccattgc
    11401 tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc ggcaggcttt attgttaccg
    11461 ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt tcccctcggt gatatgtttg
    11521 aacgccgccg cctgattgtc tcgatgacct tactggcggc atgcggcatg ttgattaccg
    11581 ccagcagtca gtcgctggcg atgatgatcc tcggtacggc attaaccggt ttattctcag
    11641 tcgtggcaca aattctggtt ccactggcag cgacgctggc ttcaccggat aaacgcggca
    11701 aagtggttgg caccattatg agcggcctgc tgttggggat attgctggca cgaaccgttg
    11761 ccgggttact ggcaaacctc ggcggctggc gcaccgtctt ttgggttgcc tcggtgttaa
    11821 tggctctgat ggcgctagca ttatggcgtg gtctgccaca aatgaaatca gaaacccacc
    11881 tcaattaccc acagttgttg ggttccgttt tcagtatgtt tatcagcaat aagatcctgc
    11941 gcacccgcgc gttgctgggc tgcctgacct ttgccaactt cagcattctc tggacctcaa
    12001 tggccttttt gcttgccgca ccgcctttta attacaacga tggcgtcatt ggtctgtttg
    12061 gacttgcggg ggctgccgga gcgttgggcg ctcgtccggc gggcggtttt gccgacaagg
    12121 gcaaatcgca ccacaccaca actttcggtc tgctgctgct gttactttca tggctggcaa
    12181 tctggtttgg tcacacttcc gtactggcgt tgattatcgg cattctggtg ctggacctca
    12241 ccgtacaggg cgtgcatatc actaaccaga cggtaattta tcgaatacat cctgatgcgc
    12301 gtaatcgcct gaccgcaggt tacatgacca gctactttat tggcggtgcc gccggttcgc
    12361 taatttcagc ctcagcctgg caacatggcg gttgggctgg cgtttgtctg gctggcgcga
    12421 cgattgccct ggttaactta ctggtctggt ggcgaggttt tcatcgtcag gaagccgcaa
    12481 attaagcaat cagcaaccgt tttcgtggct ttacacttat aagggtgtta agaagcccat
    12541 cagtctgata aggttaagat attcattcag tctatttata atattaacaa tcgttaagcg
    12601 tacactctat ggaaagccct actacacagc ctgctcctgg ttcggcgacc ttcatggaag
    12661 gatgcaaaga cagtttaccg attgttatta gttatattcc ggtggccttt gcgttcggtc
    12721 tgaatgcgac ccgtctggga ttctctcctc tcgaaagcgt ttttttctcc tgcatcattt
    12781 atgcaggcgc gagccagttc gtcattaccg cgatgctggc agccgggagt agtttgtggg
    12841 ttgctgcact gaccgtcatg gcaatggatg ttcgccatgt gttgtatggc ccgtcactgc
    12901 gtagccgtat tattcagcgt ctgcaaaaat cgaaaaccgc cctgtgggcg tttggcctga
    12961 cggatgaggt ttttgccgcc gcaaccgcaa aactggtacg caataatcgc cgctggagcg
    13021 agaactggat gatcggcatt gccttcagtt catggtcatc gtgggtattt ggtacggtaa
    13081 taggggcatt ctccggcagc ggcttgctgc aaggttatcc cgccgttgaa gcagcattag
    13141 gttttatgct tccggcactc tttatgagtt tcctgctcgc ctctttccag cgcaaacaat
    13201 ctctttgcgt taccgcagcg ttagttggtg cccttgcagg cgtaacgcta ttttctattc
    13261 ccgtcgccat tctggcaggc attgtctgtg gctgcctcac tgcgttaatc caggcattct
    13321 ggcaaggagc gcccgatgag ctatgaggtt ctgctgcttg ggttactggt tggcgcggcg
    13381 aattattgct tccgctattt gccgctgcgc ctgcgtgtgg gtaatgcccg cccaaccaaa
    13441 cgtggcgcgg taggtatttt gctcgacacc attggcatcg cctcgatatg cgctctgctg
    13501 gttgtctcta ccgcaccaga agtgatgcac gatacacgcc gtttcgtgcc cacgctggtc
    13561 ggcttcgcgg tactgggtgc cagtttctat aaaacacgca gcattatcat cccaacactg
    13621 cttagtgcgc tggcctatgg gctcgcctgg aaagtgatgg cgattatata acccacaaga
    13681 atcatttttc taaaacaata catttacttt atttgtcact gtcgttacta tatcggctga
    13741 aattaatgag gtcataccca aatggatagt tcgtttacgc ccattgaaca aatgctaaaa
    13801 tttcgcgcca gccgccacga agattttcct tatcaggaga tccttctgac tcgtctttgc
    13861 atgcacatgc aaagcaagct gctggagaac cgcaataaaa tgctgaaggc tcaggggatt
    13921 aacgagacgt tgtttatggc gttgattacg ctggagtctc aggaaaacca cagtattcag
    13981 ccttctgaat taagttgtgc tcttggatca tcccgtacca acgcgacgcg tattgccgat
    14041 gaactggaaa aacgcggttg gatcgaacgt cgtgaaagcg ataacgatcg ccgctgcctg
    14101 catctgcaat taacggaaaa aggtcacgag tttttgcgcg aggttttacc accgcagcat
    14161 aactgcctgc atcaactctg gtccgcgctc agcacaacag aaaaagatca gctcgagcaa
    14221 atcacccgca aattgctctc ccgtctcgac cagatggaac aagacggtgt ggttctcgaa
    14281 gcgatgagct aacgcgtcat ctcgctcaaa aatccagatt tataaaagaa aaaatgaccg
    14341 gccagcatcg caacatgctg gcctttttgg caagcaggtc ggctcagccg atgagttaag
    14401 aagatcgtgg agaacaatat gagcgcaaat gcggagactc aaaccccgca gcaaccggta
    14461 aagaagagcg gcaaacgtaa gcgtctgctc ctccttctca ccttgctctt tataattatt
    14521 gccgtagcga tagggattta ttggttttta gtactgcgtc atttcgaaga aaccgatgac
    14581 gcatacgtgg cagggaatca aattcaaatt atgtctcagg tgtctggcag cgtgacgaaa
    14641 gtctgggccg ataacaccga ttttgtaaaa gaaggcgacg tgctggtcac tctcgacccg
    14701 acagatgctc gccagacgtt tgaaaaagcc aaaactgcac tggcttccag cgttcgccaa
    14761 acccaccagc tgatgattaa cagcaaacag ttgcaggcga atattgaggt gcaaaaaatc
    14821 gccctcgcga aagcacaaag cgactacaac cgccgtgtgc cgctgggcaa tgccaacctg
    14881 attggtcgcg aagagctgca acacgcccgc gacgccgtta ccagtgccca ggcgcaactg
    14941 gacgtcgcga ttcaacaata caatgccaat caggcgatga ttctggggac taaactggaa
    15001 gatcagcctg ccgtgcaaca ggctgccacc gaagtacgta acgcctggct ggcgctggag
    15061 cgtactcgta ttgtcagtcc gatgaccggt tatgtctccc gccgcgcggt acagcctggg
    15121 gcgcaaatta gcccaacgac gccgctgatg gcggtcgttc cagccaccaa tatgtgggtg
    15181 gatgccaact ttaaagagac gcagattgcc aatatgcgta tcggtcagcc ggtcactatc
    15241 accaccgata tttacggcga tgatgtgaaa tacaccggta aagtggttgg tctggatatg
    15301 ggcacaggta gcgcgttctc actgcttccg gcgcaaaatg cgaccggtaa ctggatcaaa
    15361 gtcgttcagc gtctgcctgt tcgtatcgaa ctggaccaaa aacagctgga gcaatacccg
    15421 ctgcgtatcg gtttatccac gctggtgagc gtcaatacca ctaaccgtga cggtcaggta
    15481 ctggcaaata aagtacgttc cacaccggta gcggtaagca ccgcgcgtga aatcagcctg
    15541 gcacctgtca ataaactgat cgacgatatc gtaaaagcta acgctggcta atccagaggt
    15601 gcgtgtgatg caacagcaaa aaccgctgga aggcgcgcaa ctggtcatta tgacgattgc
    15661 gctgtcactg gcgacattca tgcaggtgct ggactccacc attgctaacg tggcgatccc
    15721 cactatcgcc gggaatctgg gctcatcgct cagccaggga acgtgggtaa tcacttcttt
    15781 cggggtggcg aatgccatct cgatcccgct taccggctgg ctggcaaagc gcgtcgggga
    15841 agtgaaactg ttcctctggt ccaccatcgc ctttgctatt gcgtcgtggg cgtgtggtgt
    15901 ctccagcagc ctaaatatgc tgatcttctt ccgcgtgatt caggggattg tcgccgggcc
    15961 gttgatcccg ctttcgcaaa gtctattgct gaataactac ccgccagcca aacgctcaat
    16021 cgcgctggcg ttgtggtcga tgacggtgat tgtcgcgcca atttgcggcc cgatcctcgg
    16081 cggctatatc agtgataatt atcactgggg ctggatattc ttcatcaacg tgccgattgg
    16141 cgtggcggtg gtgttgatga cattgcagac gctgcgcgga cgcgaaaccc gcaccgaacg
    16201 gcggcggatt gatgccgtgg ggctggcact gctggttatt ggtatcggca gcctgcagat
    16261 tatgctcgac cgcggtaaag agctggactg gttttcatca caggaaatta tcatccttac
    16321 cgtggtggcg gtggtggcta tctgcttcct gattgtatgg gagctgaccg acgataaccc
    16381 gatagtcgat ctgtcgttgt ttaagtcgcg caacttcacc atcggctgct tgtgtatcag
    16441 cctcgcgtat atgctctact tcggcgctat tgttctgctg ccgcagttgt tgcaggaggt
    16501 ctacggttac acggcaacgt gggcaggtct ggcctctgcg ccggtaggga ttattccggt
    16561 gatcctgtcg ccgattatcg gccgcttcgc gcataaactg gatatgcgcc gactggtaac
    16621 cttcagcttt attatgtatg ccgtctgctt ctactggcgt gcctatacct ttgaaccggg
    16681 tatggatttt ggcgcatcgg cctggccgca gtttatccag ggcttcgctg tggcctgctt
    16741 ctttatgccg ctgaccacca ttacgctgtc tggtttgcca ccggaacgac tggcggcggc
    16801 atcgagtctc tccaacttta cgcgtacgct ggcggggtct atcggcacgt cgataaccac
    16861 aaccatgtgg accaaccgcg agtcgatgca ccatgcgcag ttaaccgagt cggtaaaccc
    16921 gttcaacccg aatgcccagg cgatgtacag tcaactggaa gggcttggga tgacgcaaca
    16981 gcaggcctcc ggctggattg cccagcagat cactaaccag gggctgatta tttccgccaa
    17041 tgagatcttc tggatgtcag ccgggatatt cctcgtcctg ctggggctgg tgtggtttgc
    17101 taaaccgccc tttggcgcag gtggcggcgg aggcggtgcg cactaagtac aactaagcca
    17161 gttcatttga actggctttt ttcaattaat tgtgaagata gtttactgac taaatgtgca
    17221 gttcctgcaa cttctctttc ggcagcgcca gttcttcgtt gctgttgatg cgcacgtcac
    17281 gttccagaat gctacgcgca atatcctgcg cttcctgcaa cgagtgcatc tggtaagtgc
    17341 cacactggta gacgttcagc tccggaatct gattctgatc ctgcactttc agcacgtctt
    17401 ccattgccgc tttccaggca tcagcaacac gctgctcatc tggcgtacca atcagactca
    17461 tataaaaacc ggtgcggcag cccattggcg agatatcgat aatctctaca ccattaccgt
    17521 taagatggtt acgcataaaa ccagcaaaca ggtgctccag ggtatggatc cctctttctg
    17581 gcatcacttc tttgttcggc acgcagaagc gcagatcgaa cacggtgatt gcgtcgccat
    17641 gcggggtgtt cattgttttc gccacccgaa ctgcaggcgc ttccatccgg gtatgatcga
    17701 ctgtgaagct atctaacaac ggcatttagc cacctccggt aattttttta aaaattttct
    17761 gaactctttc ttcccaggcg agtctgagta tatgaaagac gcgcatttgt tatcatcatc
    17821 cctgaattca gagatgaaat tttggccact cacgagtggc ctttttcttt tctgtcaggc
    17881 gtgtttttcc agccacaccg caaacggttc ggtatctgcg gcttccattt cctgctgacg
    17941 gcgttcagac gcctcgcgct cggctacaaa atcctcttcg cgcagaattt ccagcggctc
    18001 ttcacgcagc agattacggt aggcttctgc aaatgctttg cctgttccgc caataccagt
    18061 atcaatcata gaccttaaga tacgggcaga gaaagtcaga tcgggattat cgaagcaggc
    18121 gaccagttca tcacacactt tctgatacgc ttcgccgccg ttaatgctat ccagcgtttg
    18181 cgcgacgcgt ttcagatcgc ggaacagatc tttacccacc tgcggtaacg ggaactgcgc
    18241 ggtttcgcag ccgataccca gcgtcagacc cggtttgcga ccttcgagga tcacccggtt
    18301 ccagttaacg cgtgtacagg caagttcgct actgctcatt tccggtgcat cagccagcgc
    18361 acaccagacc ataaacaggt cgaggaatcg cacctgctgt tcatctacac caatcggcga
    18421 gaacgggttg atgtccagcg aacgcacttc aatatattca atgccgccac gtaacagcgc
    18481 atcagaaggc gactcgccgc tgcgggtaac gcgttttgga cgaatcggcg cgtacagttc
    18541 gttttcaatc tgcagcacat tgctattgat ttgcagccgc ttaccatctt tctcaatacc
    18601 aatcttcgcg tactcttccg atggcgtttt gattgcctgt ttcaggcccg ctacgtactc
    18661 gtaaagatcg ttgaaggtaa taccaagatt gctttgcgat ttattggtat agccaagatc
    18721 gctcaaacga agagaggtcg catacggcag gtaatacata ccgcactcgg ttttctcaaa
    18781 cggcagcgac gttggtttac cttgcaggaa tgaagagcat attgccggag acgcgccaaa
    18841 cagataagga atgacccaac cgaagcgata gtagttgcga ataacgcgga aatagcccgc
    18901 agagattttc tctttagcat cagcgcccga aatatcgcca cacttcgctt gccagaatgc
    18961 cataggcaaa gagaagttgt aatgcacgcc ggagatggtt tgcatcagcg cgccgtagcg
    19021 atttttcagc ccttcacggt acagcgtttt aaagcgtccg gtgttagaag tgccatactg
    19081 cgccagttcg atgtcctgac cttctgcgat gtagcatggc atacttaacg gccacatccg
    19141 ctcatcgccc atgttgcgcg ccgtatagcg atgcaggtcg cgcataaagg tcagcatatg
    19201 ttcaatatca ccatccactg gtgtaataaa ttccagcaat gcttccgcaa aatcggtggt
    19261 tatccattta tgcgtcagtg cggaacctaa tgcttcagga tgacctgttg ttgccagtgt
    19321 gccatcagca ttaacacgca aagtttcgcg ctccagccca cgctgtatcc cctttaacgc
    19381 ctgaggatgt ttttccagcc aggccagcgc ctgtgatacg tccgggatca aattgacctc
    19441 ccgcctgtca aaatcgtttt aattagcata actgtaatgg tgaccatatg tgcaggccta
    19501 caattagtgc caccacatca tgccctgaac ggtcgctgct gcaactgcaa catagcgtaa
    19561 cgctttacca aggcacaaaa aaaagattac cggcccccag gagatgcgca tccatcccgc
    19621 taacaggcac agtaaatcgc caactacggg catccagctt aataatagcg tgactgcacc
    19681 atagcgtttc agccagccag tagctttctc ttgccagcgc gatgttttac gcaatggaaa
    19741 gaaacgccca aggataacgt tagttaaccc tccaaggcta ttacccattg ttgctgttaa
    19801 aactaaaacc cagggatgac tgatcccgga aagcaacgtt gccaccagca cgacttcgga
    19861 gttgccgggt aatagtgtag cgctgagaaa actactggca aacaacgaga aaagcgataa
    19921 cgcttcactc acagcaagcg aacatccaca gcgtccatgc ctgctgcacg ggccgcctga
    19981 ataccgaaat cggcatcttc aaagaccaca cactgcgtcg gttgcacgcc catacgctgc
    20041 gcgcacaaca aaaatgtgtc tggcgcgggt ttatggtgtt tgacgtgatc ggcagcgacg
    20101 acggcgtcaa aataacggcg taatcccagg tgagccagca atgcctcagc gatggcgctt
    20161 tcactccccg ttcctacagc cattgggcga cgaccatgcc aacttttcac cacttcaaca
    20221 agaggaagcg gctcgacact atccagcagc atacttctta ctgcttctgt tttttcacgc
    20281 gctaacgcat gcgggtcgag atcggcctga ttcagctcaa taattgcctg agcaatacgc
    20341 caggtgggcg atccattaag cgcaatcatc gcctgaacat cgtactgaag accgtagtgc
    20401 cctaatactt cgcgccacgc tttacggtgc gtaggctccg tatccaggat tgtgccatcc
    20461 atatcaaaaa ttaaacctgc ataacgctcg tacatggtct tctcgcaagt cgaaaaatat
    20521 gacgctactt tagcgtaatt gcttgatttt gtcgctgatg gcgaaggaga agaaaattca
    20581 ggaaatggat aaagtagtta tttagaaatg agatattttt gaaggaaatt ttttggag
//
LOCUS       DAZKFU020000068        20584 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZKFU020000068 DAZKFU020000000
VERSION     DAZKFU020000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.068"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..850
                     /locus_tag="ACHOQQ_RS09815"
                     /old_locus_tag="pgaptmp_001963"
     CDS             <1..850
                     /locus_tag="ACHOQQ_RS09815"
                     /old_locus_tag="pgaptmp_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGU4470221.1"
                     /translation="MGLSGRIITCKEGIYYLAKIGHYLLHDTMTRVNMDFTQDVCYQG
                     LFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHYYSDGAFNAALPY
                     VFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKENIANAGFSDRID
                     FHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKPGAKVCIMELFWD
                     AQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVAERHDNLGVGHTL
                     LICQKK"
     gene            891..1613
                     /locus_tag="ACHOQQ_RS09820"
                     /old_locus_tag="pgaptmp_001964"
     CDS             891..1613
                     /locus_tag="ACHOQQ_RS09820"
                     /old_locus_tag="pgaptmp_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HGU4470222.1"
                     /translation="MKFTLNITDWQALAPGLSEAQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDTFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            1610..2431
                     /locus_tag="ACHOQQ_RS09825"
                     /old_locus_tag="pgaptmp_001965"
     CDS             1610..2431
                     /locus_tag="ACHOQQ_RS09825"
                     /old_locus_tag="pgaptmp_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGU4470223.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            2406..2663
                     /locus_tag="ACHOQQ_RS09830"
                     /old_locus_tag="pgaptmp_001966"
     CDS             2406..2663
                     /locus_tag="ACHOQQ_RS09830"
                     /old_locus_tag="pgaptmp_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HGU4470224.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            2675..2926
                     /locus_tag="ACHOQQ_RS09835"
                     /old_locus_tag="pgaptmp_001967"
     CDS             2675..2926
                     /locus_tag="ACHOQQ_RS09835"
                     /old_locus_tag="pgaptmp_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4470225.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPKDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            2931..3512
                     /locus_tag="ACHOQQ_RS09840"
                     /old_locus_tag="pgaptmp_001968"
     CDS             2931..3512
                     /locus_tag="ACHOQQ_RS09840"
                     /old_locus_tag="pgaptmp_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470226.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQPRRQAGPLQAVTQIVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLVRRQVMKRDRA"
     gene            3509..4870
                     /locus_tag="ACHOQQ_RS09845"
                     /old_locus_tag="pgaptmp_001969"
     CDS             3509..4870
                     /locus_tag="ACHOQQ_RS09845"
                     /old_locus_tag="pgaptmp_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HGU4470227.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRVIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGLLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQCIGVLLVLDDE
                     AHLQWQNGGGHSQEQAWRRLLHPMLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            4857..5210
                     /locus_tag="ACHOQQ_RS09850"
                     /old_locus_tag="pgaptmp_001970"
     CDS             4857..5210
                     /locus_tag="ACHOQQ_RS09850"
                     /old_locus_tag="pgaptmp_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HGU4470228.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            5201..6877
                     /locus_tag="ACHOQQ_RS09855"
                     /old_locus_tag="pgaptmp_001971"
     CDS             5201..6877
                     /locus_tag="ACHOQQ_RS09855"
                     /old_locus_tag="pgaptmp_001971"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4470229.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            6881..7303
                     /locus_tag="ACHOQQ_RS09860"
                     /old_locus_tag="pgaptmp_001972"
     CDS             6881..7303
                     /locus_tag="ACHOQQ_RS09860"
                     /old_locus_tag="pgaptmp_001972"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HGU4470230.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            7300..7905
                     /locus_tag="ACHOQQ_RS09865"
                     /old_locus_tag="pgaptmp_001973"
     CDS             7300..7905
                     /locus_tag="ACHOQQ_RS09865"
                     /old_locus_tag="pgaptmp_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HGU4470231.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            7874..10192
                     /locus_tag="ACHOQQ_RS09870"
                     /old_locus_tag="pgaptmp_001974"
     CDS             7874..10192
                     /locus_tag="ACHOQQ_RS09870"
                     /old_locus_tag="pgaptmp_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HGU4470232.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVVLALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKFTTSLENA
                     KKEGLISNYRTIPLNSLARQEEDLHLLTTAAPTATKALQNAGLTTVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKNSAALQEISSYYPCGIAWVDRKNTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAITLAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            10189..10773
                     /locus_tag="ACHOQQ_RS09875"
                     /old_locus_tag="pgaptmp_001975"
     CDS             10189..10773
                     /locus_tag="ACHOQQ_RS09875"
                     /old_locus_tag="pgaptmp_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HGU4470233.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            10775..11944
                     /locus_tag="ACHOQQ_RS09880"
                     /old_locus_tag="pgaptmp_001976"
     CDS             10775..11944
                     /locus_tag="ACHOQQ_RS09880"
                     /old_locus_tag="pgaptmp_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HGU4470234.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPVIPEKFAAHRSRNNQILLAALAQIQPQIDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSPTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQLIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            11941..12405
                     /locus_tag="ACHOQQ_RS09885"
                     /old_locus_tag="pgaptmp_001977"
     CDS             11941..12405
                     /locus_tag="ACHOQQ_RS09885"
                     /old_locus_tag="pgaptmp_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HGU4470235.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCAAGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            12405..13136
                     /locus_tag="ACHOQQ_RS09890"
                     /old_locus_tag="pgaptmp_001978"
     CDS             12405..13136
                     /locus_tag="ACHOQQ_RS09890"
                     /old_locus_tag="pgaptmp_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HGU4470236.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            13133..14362
                     /locus_tag="ACHOQQ_RS09895"
                     /old_locus_tag="pgaptmp_001979"
     CDS             13133..14362
                     /locus_tag="ACHOQQ_RS09895"
                     /old_locus_tag="pgaptmp_001979"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HGU4470237.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            14364..14951
                     /gene="acpT"
                     /locus_tag="ACHOQQ_RS09900"
                     /old_locus_tag="pgaptmp_001980"
     CDS             14364..14951
                     /gene="acpT"
                     /locus_tag="ACHOQQ_RS09900"
                     /old_locus_tag="pgaptmp_001980"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGU4470238.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            15062..16636
                     /gene="nikA"
                     /locus_tag="ACHOQQ_RS09905"
                     /old_locus_tag="pgaptmp_001981"
     CDS             15062..16636
                     /gene="nikA"
                     /locus_tag="ACHOQQ_RS09905"
                     /old_locus_tag="pgaptmp_001981"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4470239.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            16636..17580
                     /gene="nikB"
                     /locus_tag="ACHOQQ_RS09910"
                     /old_locus_tag="pgaptmp_001982"
     CDS             16636..17580
                     /gene="nikB"
                     /locus_tag="ACHOQQ_RS09910"
                     /old_locus_tag="pgaptmp_001982"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGU4470240.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            17577..18410
                     /gene="nikC"
                     /locus_tag="ACHOQQ_RS09915"
                     /old_locus_tag="pgaptmp_001983"
     CDS             17577..18410
                     /gene="nikC"
                     /locus_tag="ACHOQQ_RS09915"
                     /old_locus_tag="pgaptmp_001983"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGU4470241.1"
                     /translation="MNFFISSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPNAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            18410..19174
                     /gene="nikD"
                     /locus_tag="ACHOQQ_RS09920"
                     /old_locus_tag="pgaptmp_001984"
     CDS             18410..19174
                     /gene="nikD"
                     /locus_tag="ACHOQQ_RS09920"
                     /old_locus_tag="pgaptmp_001984"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGU4470242.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAAALGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLRTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            19171..19977
                     /gene="nikE"
                     /locus_tag="ACHOQQ_RS09925"
                     /old_locus_tag="pgaptmp_001985"
     CDS             19171..19977
                     /gene="nikE"
                     /locus_tag="ACHOQQ_RS09925"
                     /old_locus_tag="pgaptmp_001985"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGU4470243.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLERASEMLKAVDLDDSVLNKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            19983..20384
                     /gene="nikR"
                     /locus_tag="ACHOQQ_RS09930"
                     /old_locus_tag="pgaptmp_001986"
     CDS             19983..20384
                     /gene="nikR"
                     /locus_tag="ACHOQQ_RS09930"
                     /old_locus_tag="pgaptmp_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGU4470244.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
BASE COUNT         4423 a         5794 c         5725 g         4642 t
ORIGIN      
        1 tatgggctta agcgggcgaa ttattacctg taaagaggga atttattacc tggcgaaaat
       61 cggtcattac ctcctgcatg acactatgac tcgcgtgaat atggatttca cccaggatgt
      121 ctgttaccag gggctcttct tcctcgccga ttcactcaat gaaggaaaac cttccgggtt
      181 aaaagtattt ggcgactggt cgacgatata tccggcactt tcgcaattac cagacgccgc
      241 tcgtgaaagc tggtttgcgt ttgatcatta ctattcagat ggcgcattta acgccgcact
      301 gccttatgta ttcgccaaca accccacaac attgtacgat gtaggcggca atacgggtaa
      361 gtgggctttg cgttgctgca aatacaatga aaacatcgcc gtgacgttat tagatctgcc
      421 acaacaaatc gtactagcga aagaaaatat cgcgaatgca ggtttttctg atcgcataga
      481 tttccatgcg gtggatatgc taagcgatgc cccattaccg ggcgaggctg atatctggtg
      541 gatgagccaa tttttagact gtttttcacc cgaacaaata attagcatac tcagcaaaat
      601 tgccagcgta atgaagcctg gcgcaaaagt ttgcatcatg gagttgttct gggatgcgca
      661 gcggtttgaa gcagcatcat ttagcctgaa cgcgtcctca ctttatttta cctgcatggc
      721 aaacggtaac agccgtttct atagtgcaga gaaattttac gactatctga ataaagcagg
      781 attccaggta gcagagcgcc acgataattt aggcgttggg catacacttt tgatatgcca
      841 aaagaaataa tcgtcactta ataattttgg tttaccgcgg gcgcgcgttg atgaaattta
      901 cattaaatat taccgactgg caggcactgg cgcctggact tagcgaagcg cagcaatggc
      961 aagcatggtc gcgccagccg tgggcgattg accctgccgc gccgctggca aaattaagtg
     1021 aactgccaat gatgaccgcc cgccgcctca gttcaggcag caaactggct gttgaatgcg
     1081 gtctggcaat gctacgccgc catcagccag acgccgtttt gtataccagt cgtcatggcg
     1141 aactggagcg caattaccgc attgttcatg cactggcaac cgaacaggcg ctttctccga
     1201 ccgactttgc actttcagta cacaactctt ccgtgggtaa cctgaccatt gcggccaaac
     1261 agccgatcgt ttcgtcatcg ctttctgctg gtcgcgacac tttccagcaa ggtttgtgtg
     1321 aagtgcttag cctgttacaa gcgggatacc agcgcgtgct gatggtcgat tttgacggtt
     1381 tcctgcccga gttttatcac ccacaactgc ctgcagaaat gccgacctgg ccgtacgccg
     1441 tcgcgctggt gattgaagct ggcgatgact ggcaatgtga aacgcagtca gccattgcgg
     1501 gcaatgaaac aacactgccg caaagcatgt tgtttttaca gcactattta caaaatgtag
     1561 acacattttc actgcctggc gagcgcgtgc aatggcgctg gagccacaga tgagtaagct
     1621 gatgtcacgt ctggagtgga cctggcgact ggtcatgacc gggttatgtt ttgccctgtt
     1681 tggtctcggc ggcctgctgc tttctgtcgt gtggtttaac atattgttag ttttggtgtg
     1741 ggatacttcc cgccgccgtc gtttggcgcg tcgcagcatt gccgccagtt ttcgcctgtt
     1801 tttaactgtc gcgaaagggc ttggcgtgct ggattatcgt attgatggcg ctgaaatttt
     1861 acgtcaggaa cgcggttgcc tcgtcgtagc caatcatccc acgctgatcg attacgtgct
     1921 gctggcgtca gtcatgccag aaactgactg cctggtgaaa agcgcactgt taaaaaatcc
     1981 ttttcttggc ggcgtcgtgc gggcggcaga ttacttaatc aacagcgaag ctgaaaccct
     2041 gttaccccgc tgccaacagc gactggcaca gggtgacact atcctgattt tccccgaagg
     2101 cacacgaacg aagcccggtg aaaaaatgac gctgcagcgc ggggcggcga atattgccgt
     2161 gcgctgcgcc agcgatttac gcatcgtgac aatcggctgt agcgagcatc tgctggataa
     2221 acaaagtaaa tggtatgacg ttccacctga ccggccgttt tttacggttg cagtccatgg
     2281 tcgggttgaa atcaataaat tttacgatgc aacttcacaa gaaccggcgc tggcagcaag
     2341 gcagttaaac cggcatcttt tgcttcaatt acaacaaggt tgtttacctc tctcaggaat
     2401 aaataatgca agcgctttat ctggaaatta aaaacctcat catcagcacc ctcaatctgg
     2461 acgagttgac gcctgatgat attgataccg acgcgccgct gtttggcgac ggtttagggc
     2521 tggactctat cgatgccctg gaactgggac tggcagtaaa aaatgagtac ggcatcgtgc
     2581 tctctgcgga aagcgaagag atgcgtcagc actttttctc cgtcgccact ctggcctctt
     2641 tcattgctgc gcaacgtgcc tgaggaaagc catcatgaca gaacaacaaa ccgtctatca
     2701 ggaagtctca gccctgctgg ttaagctgtt tgaaatcgat ccgaaggaca tcaaacctga
     2761 agcgcgcctg tacgaagatc tggaactgga cagcatcgac gccgttgaca tgattgtgca
     2821 cctgcaaaag aaaaccggta agaaaatcaa gccggaagag tttaaagcag tgcgtacagt
     2881 ccaggatgtc gtcgaggctg tagaacgcct gctacaagaa gcctgatctg atgtcgggca
     2941 ttcgctcgct accgatgatt aagctgctga ctggattatt gctgctcgcc tggccgtttg
     3001 tgatttggtt tggcttagcg cataacggcc tgcactggct gttaccgcta atggcgttgc
     3061 tgcttttgtt gcgtctgcgc cagccccgtc gccaggcggg gcctttacaa gccgtcacac
     3121 aaatcgtcgc tgtcgtgggc atcgcattat gtgtcgccag ctttatgttg aaaacgcacc
     3181 agctactgct gttttacccg gtagtggtta acgcggtcat gctggcggtt tttggcggtt
     3241 cgctgtggtc ggcaatgccg atagttgagc ggctggcgcg gttgcaggag ccagacctcc
     3301 cggaaaaggg cgtgcgctat acccgccacg tcacgcaaat ctggtgcggc tttttcatca
     3361 ttaacggtgg aattgccctc tttaccgcgc tttatgccga tatgtcgtta tggaccgcct
     3421 ggaacggcat gattgcttat ttgttgatgg ggacgttaat ggctggtgaa tggctggtgc
     3481 gccgccaggt tatgaaaaga gatcgcgcat gaagcagacc ttaccgcttg cacgctggct
     3541 gactgcgcca cgccctgatg acacacccat tgcgtggctg aacgaaagta cctggacgct
     3601 gggcgatttg cgtcacgatg tcgcgcaact tatctgccgc ttgcagcaac agccgggtga
     3661 acgctgggcg ctgtgctttg agaacagcta tctgtttatc gttgccctgc tcgccacgct
     3721 acacgccgga aaaacaccgg tgcttccggg gcataaccgt gtcatccagc ttaacgagca
     3781 acgtgaactg ttcgatggtg tgcttagcga cagcgaactt aactggcaag gttcattgct
     3841 gttggtagca agttctccac aaatcgcgac acaatccttc acctttgccg ctatagcgcc
     3901 agaggctttt atcgagctgt ttacctccgg ttccacggga cagccgaaac gggtgattaa
     3961 acctgttcat ttgctggatc gtgaagcaga actgctggct gaacgattag gcgcacgtct
     4021 ggcgggttgt cgtgtcgtcg gttccgtatt gccgcagcat ttgtacggcc tgacttttcg
     4081 cgttttcctg cccatggcgt tgggattacc gctgcacgcc gccatgctct ggtatgtcga
     4141 acagtttgcc gcgttgagtc atcagcatcg ctatatcttc atcagcagcc cggcattttt
     4201 gaagcgcctg gatacccagc tttctccgcc acccgttcag gtgcttctct ctgctggcgg
     4261 tgagctgccg tggcaggatg tacaacacac cgcaagctgg ctgcgcgtct ggcctgatga
     4321 aatctacggc agcacggaaa ccggcgtcat cgcctggcgc taccgcgaac aagagcaacg
     4381 ccgctggcta cccctcccag gtatgcagtt ccaggccgaa ggcgatgcct ttcgcctctt
     4441 ttcaccgctg atagcggaag ataacggcct gttactcgac gatatcttac agtttagcga
     4501 agatggtcag ttccatctga tggggcgtcg tgggcgcatc gttaagattg aagaaaaacg
     4561 tatttctttg caggaagtgg aacaacgtct gctggcactg gacggcattc gcgaggcggc
     4621 ggcagttcct gttacacgcg gcgggcgtca gtgcattggc gttttgctgg tgctggatga
     4681 tgaagctcat ctgcaatggc agaacggcgg cgggcatagc caggagcagg cctggcggcg
     4741 attactgcac ccgatgctgg agccggttgc tattccgcgt tactggcgcg ttattgatga
     4801 aatgccagta aacagtatga acaagcgtgt ctatgcgcaa ttacaggagt tatttcatga
     4861 agccccatga aatcgagcgc catcaggcac aaacgaatca ccttgaaatt gttctgcatc
     4921 tcagggcgga tctgttctgg tttcgcggtc attttgccgt gcaaccatta ctcccaggag
     4981 ttgcgcaaat cgactgggcg atgagctacg cgctcaatct gctcgcgcct ggctggcgtt
     5041 ttcactccat tcagaacata aaattccagt ctccgttgct gccggaaaac cgcgtcacgc
     5101 tcgcgcttaa ctggcaggaa gaacgccaga ttctgagctt tagctatcag cgtcacgacg
     5161 gtgacgcccg ccacaccgcc agtagcggga agatccgcct atgtcggtaa acttttctcc
     5221 ctgcgtgttg ataccctgct acaaccacgg cgcgatgatg ccgggcgtgc tggcgcgtct
     5281 taagccattt aatctgccct gtattgtagt ggatgacggc agcgatgccg ccacgcaaca
     5341 gcaactggac agtctgcttg ccgaacagcc tggcgtgacc ttaattcgct tagcagaaaa
     5401 cgcaggtaaa ggcgcggcag tgatgcgtgg cttacaggca gcggcagacg cagggttcag
     5461 ccatgcggtg caggtggatg ctgacggtca acatgcgatt gaagatatcc ccaaactgct
     5521 ggcactcgct gaacaacacc ctgcggcgct gatctccggc cagccaattt acgatgactc
     5581 catcccccgc tcacggcttt acgggcgctg ggtcacccac gtctgggtat ggatcgaaac
     5641 gctctccctg caactgaaag acagcatgtg cggctttcgc gtttatccgg ttgcgccaac
     5701 gctgcaactg gcaaaacacg ccaccatcgg caagcggatg gatttcgaca ctgaagtgat
     5761 ggtgcgcctc tactggcagg gaaacaccag ctatttcgtg ccgacccgcg tcacctatcc
     5821 actggacggg ctttcgcatt ttgatgccct gaaagataac gtccgcatct cgctcatgca
     5881 cacgcgtctg tttttcggca tgttgccgcg tattccttca ctgctgatgc gccgctcttc
     5941 cagccactgg gcgcggcaga gtgaagtgaa aggattatgg ggaatgcgcc tgatgctgct
     6001 ggtctggcgt ctgctgggaa gaacggcgtt tagcgcgctg ctttacccgg tggtgggcgt
     6061 ctactggctc actgcttctc gtgcgcgcaa agcgtcgcaa gactggctcg cccgtgtacg
     6121 acagcatcaa ccacaggcgg caaaactcaa cagctatcag cactttctac gtttcggtaa
     6181 tgccatgctc gacaaaatcg ccagctggcg cggcgagcta caactggggc gtgatgtgct
     6241 gtttgcgcca ggcgcagaag cggcgcttga cgtccgcgat ccgcgcggca aattgctgct
     6301 ggcctcgcat cttggcgatg tggaagtgtg ccgggcgctg gcaaaaattc agggctacaa
     6361 aaccattaac gcgctggtgt ttagcgaaaa cgcccaacgc tttaaacaga taatgcagga
     6421 gatggctcct caggcaggca ttaacctgat gccggtaaca gatatcggcc cggaaaccgc
     6481 catcctgctg aaagagaagc tggataacgg cgaatgggtg gcgattgtcg gtgaccgcat
     6541 cgccgtcacc ccgcaacgcg gcggcgactg gcgcgtctgc tggagtccgt ttatgggcca
     6601 gcctgcgccc ttcccacagg ggccgtttat tctcgcctct attttgcgct gcccggtgaa
     6661 tctgattttc gccctacgcc agcatggcaa gctgcatatt cactgcgaaa cctttgccga
     6721 cccgctgctg ttgccgcgtg gcgaacgcca acaggcgctg caaaacgcta tcgaccacta
     6781 cgccgcgcgt ctggaacatt acgcgctcca gtcgcccctc gactggttta attttttcga
     6841 tttctggcaa ctgccggaaa ttcaggacaa ggagtaaagg gtgcttaacg atccccgatt
     6901 taccgctgaa gtcgagctga ccattccctt tcacgacgtc gatatgatgg gcgtggcctg
     6961 gcacggtaac tattttcgct actttgaagt ggcccgcgag gcgctgctca accagttcaa
     7021 ttacggctat cggcagatga aagagtccgg ttatttgtgg ccagtggtcg atgcgcgggt
     7081 gaaatatcgc cacgccctca cctttgagca acgcatccgc gtgcgtgcgc atatcgaaga
     7141 gttcgaaaac cgtctgcgga ttggctatca gattttcgat gccgaaacgg gcaaacgcgc
     7201 caccaccgga tacaccattc aggtcgcggt cgacgagcaa agtcgcgaac tgtgctttgt
     7261 cagccccgat attctgtttg aacgcatggg agtcaaacca tgaaattttt accgctgctg
     7321 gcgctgctga ttagcccgtt tgtgagcgcc ctgaccctgg acgatcttca gcaacgcttt
     7381 accgaacaac cggtgatccg cgcccatttt gatcaaaccc ggacgattaa agatctgccg
     7441 cagccgctgc gatctcaggg tcagatgttg atcgcccgcg accaggggtt attgtgggat
     7501 caaacctcac cgttccccat gcagctattg ctggatgata aacgcatggt gcaggtgatc
     7561 aacggtcagc cgccgcaaat catcacggca gaaaacaacc cgcagatgtt ccagtttaac
     7621 cacctgctgc gcgcgctgtt ccaggccgat cgcaaagtgc tggaacaaaa cttccgcgtc
     7681 gaatttgctg acaaaggcga aggccgctgg acgctgcgcc tgacgccgac caccacgccg
     7741 ctggataaaa ttttcaacac catcgatctc gccgggaaaa cctatctgga gagcattcaa
     7801 cttaatgata aacagggcga tcgcaccgat attgctctta cccaacatca actgacgcca
     7861 gcgcaactga ccgatgacga acgccaacgt tttgccgccc agtaaacgcc ccgcgctgtt
     7921 atgggggcta gtctgcctgg tcatggcggc ggcgttgctg atcctgctgc cgcaatcacg
     7981 gctgaacagt agcgtgctgg ctatgttacc caaacaggct atgggcgata ttcccccagc
     8041 gctgaatgac ggctttatgc agcgtctgga ccgccaactg gtgtggctgg tcagccccgg
     8101 taaagaggct aatcccaggg tcgctcagga gtggctgacg ctgctgcaaa aatccgctgc
     8161 gctcggcgac gttaaagggc caatggatgc cgccagccag caagcgtggg gagcgttttt
     8221 ctggcagcac cgcaacggcc tgattgaccc cgacacccgc gcgcgcctgc aaaacggcgg
     8281 cgaagcgcag gcgcagtgga tcctctccca gctttattct gcgttctccg gggtaagcgg
     8341 caaggagctg caaaacgatc cgctgatgtt aatgcgcggc tcgcagctgg cgatggcgaa
     8401 aaacggccag cgtttgcggc tgatggacgg ctggctggtg acgcaggatc cccagggcaa
     8461 ctactggtat ctgctgcacg gtgaactggc gggatcgtcg tttgatatgc agcaaaccca
     8521 ccagctcatc acgaccctga atacgctgga aaaggatctg aaaacgcgtt acccgcaggc
     8581 gcagctgctt tcgcgcggca cggtgtttta cagcgattac gccagccaac aggcgaagca
     8641 ggatatctca accctgggcg tggcaacgct gctgggggtg atattgctga ttgtggcggt
     8701 gttccgctct ttgcgcccgt tgctgctttg cgtgatttcc atcggcatcg gcgcgctggc
     8761 gggaacggtc gccactttat tgattttcgg tgaattacac ctgatgacgc tggtgatgag
     8821 catgagcgtt atcggcattt ccgctgacta cacgctctat tatctcaccg agcggatggt
     8881 gcacggcaac gacgtttcac cgtggcaaag cctggcgaaa gtgcgcaatg ccttgctgct
     8941 ggcgctgctc accaccgtgg cggcgtatct gattatgatg ctcgccccct tccccggcat
     9001 tcgccagatg gcgatttttg ccgccgtcgg gttgagcgcc tcctgtctga ccgtcctgtt
     9061 ctggcatccg tggctgtgcc gtggcctgcc ggtgcgtccg gttccggcga tggcgctgat
     9121 gctacgctgg ctggcagcgt ggcggcgcaa taaaaaactg tcgcggggtc tgcccgtcgt
     9181 gctggcgctg ttttcgctgg cggggatgtc aatgctgcgc gtcgatgacg atatctcgca
     9241 gttacaggcg ctaccgcagc atattctggc ccaggagaaa gccatcaccg ctctgaccgg
     9301 gcagagcgtc gatcaaaaat ggttcgtggt ttacggcgac tccccacagc aaacattgcg
     9361 gcgactggag aaatttacca cctcgctgga gaatgcgaaa aaagaggggc ttatcagcaa
     9421 ctaccgcacc attccgctga actccctggc gcggcaggag gaagatttac atctgctgac
     9481 aacagcggcc ccgacagcaa caaaagcact gcaaaatgcc gggctgacaa cagtgaaccc
     9541 ggatctcaac gccatgccgg tgaaggttga tgaatggctg gcaagccccg ccagtgaagg
     9601 ctggcgtctg ctgtggctga cgctggaaaa tggcgaaagc ggcgtgctgg tgccggttga
     9661 aggggttaaa aatagcgcgg cgctacagga aatctccagc tattaccctt gtggcattgc
     9721 ctgggttgat cgcaaaaaca cctttgatga attgttcgca ctttaccgtt atgtgttaac
     9781 cggcttgttg ctggtggcgc tggcggtgat tgcctgcggc gcagtggccc gtctcggctg
     9841 gcgcaaaggg cttatcagcc tggtgccttc ggtgctttcg ctgggctgcg gtctggcggt
     9901 gctggcgatg agcgggcagg cggtgaatct cttttcgctg ctggcgctgg tgctggtgct
     9961 tggcatcggc atcaattaca cgctgttttt cagtaatccg cgcggcacac cattaacttc
    10021 gctactggcg atcacactgg caatgctcac caccttgctg acgctgggaa tgttggtatt
    10081 cagcgccacc caggccatca gcagttttgg cattgtgctg gtgagcggta ttttcaccgc
    10141 cttcctgctt tcgccgctgg ctatgcccga taaaaagaga acaaaaaaat gatcaaatcc
    10201 acattctggc gagcgttcgc cctgaccgct acgcttatcc tcaccggctg tagccactcg
    10261 caaccagaac aggaaggccg cccgcaggcg tggctgcaac ctggcacgcg catcacgctg
    10321 ccggcaccag ggatttcacc cgcagtcaat tcgcagcaac tgttgaccgg cagcttcaac
    10381 ggcaaaaccc agtcgctgtt agtgatgctt aacgccgacg atcagaaaat cacccttgcc
    10441 gggctgtcgt cggtcggcat tcgcctgttt ctggtgacct acgatgcgca agggctacgc
    10501 gccgagcaat ccatcgtcgt cccacagtta ccgcccgcaa gtcaggtact ggctgacgtg
    10561 atgctcagcc actggccgat tagcgcctgg caaccgcaac ttcccgcagg atggacactt
    10621 cgcgacaacg gtgacaaacg cgagctgcgt aacgccagcg gcaaactggt cacggaaatc
    10681 acctatctga atcgccaggg aaaacgcgtg ccaatcagca ttgagcagca tgtctttaaa
    10741 taccacatca ccattcaata cttaggtgac tgatatgatt tatatttccg ccgtcggcat
    10801 gatcaacgcc ctgggaaata accttgatga aatcgcaacc aacctggctc gcggtgtcgc
    10861 ccccggaatg cgcccacgcg caggctggtt gcaggggcat ccgcaggcgg tcctggctgg
    10921 cgtggatggc gagcttccgg ttatcccgga aaaattcgct gcgcaccgat cgcgcaacaa
    10981 tcagatcctg ctggcggcgc tggcgcagat ccagccgcag attgatgacg ccattgcgaa
    11041 atatggccgc gagcgcattg cgattgttct tggtaccagc acctccggcc tgcatgaagg
    11101 cgacacgcac gttaacctgc gcacccacgg gcagccgagc cccacatggc actacgcgca
    11161 acaggaactc ggcgatccgt cgcgttttct cagccactgg ctggcgctcg acggcccggc
    11221 ctataccctt tcaaccgcct gctcttccag cgccagagcg ataatcagcg ggcgcaggct
    11281 tatcgaggcg gggctggtcg atgccgccat tgtgggtggc gcagacaccc tgagccggat
    11341 gccgattaac ggttttcaca gcctcgaatc gctgtcgcca accttatgtc agccgtttgg
    11401 tcgtgaccgc gcaggtatca ccattggtga aggggcgggg ctaatgctgc tgacccggga
    11461 accgcagctc atcgcattgc tgggcgttgg tgaatcgagt gacgcttacc atatttcggc
    11521 cccgcatccg cagggcgaag gggcgatccg cgccattaat caggcactga ccgatgcgca
    11581 gcttacgcca gatgacgtcg gctatatcaa cctgcacggc accgccacgc aactcaacga
    11641 ccagattgaa tcgatggtgg ttaacgccct gtttggcgaa cgcgtaccgt gcagctccac
    11701 caaacatttg accggccaca cgctgggcgc ggcgggcatt accgaagcag caatcagtat
    11761 gttgatctta cagcgtgatt taccgctgcc cgcccaagat ttcagcctgt cgccacgcga
    11821 tcccacgctg ccaccctgcg gcattatcga aaaaccgcag ccgctggcac gtccggtgat
    11881 cctgtccaat tccttcgcct ttggcggcaa taacgccagc attctgctcg ggagggtttc
    11941 gtgagccact atttatcccc cggcgcttat ctgccccacg acgcgcccat gttactgctg
    12001 gaagacgtgg tgagcgtcag cgatgacagc gcggtgtgcc gcgtgacggt ttcgcccagt
    12061 ggcgtactgg caccgtttct cgacccggac ggcaatctcc ccggctggtt tgcccttgaa
    12121 ctgatggcgc aaaccgtcgg cgtctggtct ggctggcatc gccaccagca gggcaaaaac
    12181 agtattgagt tagggatgat actgggcgcg cgtgaattgc tttgcgccgc tggcattctg
    12241 cccgcaggcc agacgctgac cattaccgtc aaactgctga tgcaggacga tcgcttcggc
    12301 agttttgagt gcagcatcaa cgacggcgag gcgacgggcc gcattaacac cttccagccg
    12361 accgcggaag aacttaccac cctgtttcaa caaggagcgt cctgatgagt cgttcagttc
    12421 tggttactgg tgccagcaaa ggcattggtc gcgccatcgc ctgccaactg gcggcagacg
    12481 gctttaacat tggcgttcac tatcaccgtg acgccacagg cgctcaggaa acgctgaatg
    12541 ccattgtcgc caacgggggt aacgggcgtt tgctcagttt cgacgtcgca aaccgcgaac
    12601 agtgtcggga agtgctggag catgagatcg cgcaacacgg tgcctggtac ggcgtggtca
    12661 gtaacgccgg gatcgcccgc gatgccgctt ttccggcttt aagcgatgac gactgggatg
    12721 cggtgatcca caccaacctc gacagtttct acaacgtcat tcagccgtgc attatgccga
    12781 tgatcggcgc acgccagggt gggcgcatca tcacgctatc ctcggtttcc ggcgtaatgg
    12841 gcaatcgcgg ccaggtgaat tacagcgctg ccaaagccgg aattatcggc gcaacaaaag
    12901 cgctggctat cgaactcgcg aagcgcaaaa tcaccgtcaa ctgcatcgcg ccggggctta
    12961 ttgataccgg gatgatcgaa atggaagaga gcgctctgaa agaggcaatg tcgatgatcc
    13021 ccatgaaacg tatgggccag gcggaagaag ttgccgggct tgccagttat ttaatgtctg
    13081 atattgcggg ttacgtcacc cgccaggtta tttccatcaa cggagggatg ttatgacacg
    13141 tcgcgtagtg attacaggaa tgggcggcgt gactgccttt ggtgaaaact ggcaggacgt
    13201 ttctgcacgc ttactggcgt atgaaaatgc ggtgcgcaaa atgccggagt ggcaggtcta
    13261 cgatggcctg cacaccctgc ttggcgcgcc tgttgatgat ttcacgctac cggagcacta
    13321 cacccgcaag cgtatccgtg cgatgggccg cgtgtcgcaa atgtcgaccc gtgccagcga
    13381 gctggcgtta gagcaggcag ggttgattgg cgatccgata ttaaccagcg gcgaaacggg
    13441 cattgcgtac ggttcatcga ccggcagtac cggcccggta agcgagttcg ccaccatgct
    13501 gacggaaaag cacaccaata acatcactgg caccacctat gtgcagatga tgccgcacac
    13561 caccgccgtt aatactggcc tctttttcgg cctgcgcggg cgggtgatcc caacctccag
    13621 cgcctgtact tccggcagcc aggcaatcgg ttatgcgtgg gaagccattc gccacggtta
    13681 tcaaacggtg atggttgctg gtggtgccga agagttatgt ccttcagaag cagcggtatt
    13741 tgatacgctt ttcgccacca gccagcacaa tgatgcgccg aaaaccacac cttcaccgtt
    13801 cgatgaaaac cgcgacgggc tggtgattgg tgaaggcgca ggtacgctca ttctcgaaga
    13861 actggagcac gccaaagcgc gcggcgcgac catttacggt gaaatcgtcg gctttgcgac
    13921 taactgcgac gcggcacata ttacccagcc gcagcgcgaa accatgcagt attgtatgga
    13981 gcaatcgctg aaaatcgccg ggttaagcgc acaagatatc ggctatattt cagcgcatgg
    14041 tacggcgacc gatcgcggtg atatggcaga aagtctggcg acagcaacca tttacggtga
    14101 taatgtgccg ctctcctcgc tgaaaagtta ttttggtcat acgcttggtg cctgtggcgc
    14161 gctcgaagcc tggatgagtc tgcaaatgat gcgcgaaggt tggtttgcgc ctacgctaaa
    14221 cctgaataaa ccagacccga attgtggcgc gctggattac atcatgcatg aagcccgcaa
    14281 ggttgattgt gagtttttgc agagtaacaa ctttgccttt ggcggcatta atacatccat
    14341 catcattaaa cgttggccct gatatgtatc ggatagttct ggggaaagtt tcgaccttaa
    14401 gcgcagctcc actgccaccg ggtttacgcg agcaagcacc gcaaggtcca cgacgcgaac
    14461 gctggctggc ggggcgtgca ttgctttcgc acacgctttc cccgctaccg gagatcatct
    14521 atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc gctatggttc aacttaagcc
    14581 atagcggtga cgatatcgcc ctgctgttga gtgatgaagg cgaagtcggc tgcgatatcg
    14641 aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa cgccgtgttc agcctcgggg
    14701 aacacgctga aatggacgca gtgcatcctg atcagcaact ggaaatgttc tggcgcatct
    14761 ggacgcgcaa agaagccatc gttaaacagc gtggcggcag cgcctggcaa atcgtcagcg
    14821 tagatagcac ctatcactcc tcgctgtcag tcagccattg tcagctagaa aatttaagcc
    14881 ttgcgatctg cacccccacc ccctttacgc tcaccgccga cagtgtgcaa tggatcgatt
    14941 cagttaactg atccgcccac ccgactgccc atctattgat ccagaacagg taatcagtat
    15001 gacgaatact taaaatcgtc atacttattt ccgccatcta ttttaatcca ttggggttac
    15061 catgctctcc acactccgcc gcactctatt tgcgctgctg gcttgtgcgt cttttatcgt
    15121 ccatgccgct gcaccagatg aaatcaccac cgcctggccg gtgaatgtcg ggccactaaa
    15181 cccgcacctt tacacgccta accagatgtt cgcccagagc atggtttatg aaccgctggt
    15241 gaaatatcag gctgatggtt cggtgatccc gtggctggca aaaagctgga ctcattcaga
    15301 agatggtaaa acctggacct tcaccctgcg tgatgacgtg aaattctcta acggcgaacc
    15361 gtttgatgca gaggcggcgg cagaaaactt ccgcgcagtg ctcgataacc gtcaacgtca
    15421 cgcctggctg gagctggcaa accagattgt tgatgtcaaa gcactcagta aaacagagct
    15481 gcaaatcacc ctgaaaagcg cctactatcc tttcctgcaa gaactggccc tgccgcgccc
    15541 tttccgcttt atcgccccct cgcagtttaa aaaccatgaa accatgaacg ggattaaagc
    15601 gccgattggc accgggccgt gggttttgca ggaatcgaaa ctgaatcagt acgatgtctt
    15661 cgtccgtaac gaaaactact ggggcgaaaa gccggcaatt aagaagatca cttttaacgt
    15721 catcccggac ccgactaccc gcgcggtggc gtttgaaact ggcgatatcg acctgctgta
    15781 cggaaacgaa gggttattac cgctcgatac cttcgcccgc tttagccaga acccggctta
    15841 ccatacccaa ctgtcacagc cgatcgaaac cgtgatgctg gcgcttaata ccgccaaagc
    15901 ccccaccaat gagctggcag tacgtgaagc tcttaattac gcggtaaaca aaaaatcgct
    15961 gattgataac gcgttgtatg gcacccagca ggtcgccgac accctgtttg ccccttctgt
    16021 gccctacgcc aatctcggcc tgaaaccgcg ccagtacgat ccgcagaaag cgaaagcgtt
    16081 gctggaaaaa gccggttgga cgctgcctgc gggcaaagac atccgcgaga aaaatggtca
    16141 gccgctgcgc attgaactgt cgttcatcgg caccgatgcg ttaagcaaat cgatggcgga
    16201 aatcattcag gctgatatgc gccagattgg cgcagatgtc tcgctgattg gcgaagaaga
    16261 gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc atgattttcc accgcacctg
    16321 gggcgcgcca tacgacccac acgccttcct gagttcaatg cgcgtaccgt cacacgctga
    16381 cttccaggca cagcaaggat tagccgacaa accgctgatt gataaagaga tcggcgaagt
    16441 gctagcgacc catgacgaaa cgcaacgtca ggcgctgtat cgcgacattc tgacccgtct
    16501 gcatgacgag gcggtttatc tgcctatcag ttacatctca atgatggtgg tatcaaaacc
    16561 ggagctgggt aacatcccct acgcgccgat cgccaccgaa attccgttcg aacagattaa
    16621 accggtgaaa ccttaatgtt gcgttacgta ttacgccgct ttctgctgct gatcccgatg
    16681 gtgctcgccg cctcggtgat catttttctg atgctgcgcc ttggtaccgg cgacccggcg
    16741 ctcgattatt tgcgtctgtc taacctgccg ccgacgccag agatgctggc ctctacccgc
    16801 accatgctcg gactggatca gccgctgtac gtccagtacg gtacctggtt gtggaaggcg
    16861 ctgcatcttg actttggtat ctcattcgcc agccaacgcc cagtgctgga cgatatgctg
    16921 aacttcctgc ccgccacgct ggaacttgca ggtgccgcgc tggtattaat tctgctcact
    16981 tctgtgccgc tcggtatctg ggcggcgcgc catcgcgatc gtctgccgga tttcgccgta
    17041 cgtttcatcg cgtttctcgg cgtgtcgatg cctaacttct ggctggcgtt tttactggtg
    17101 atggcgtttt cggtgtatct gcaatggcta cccgcgatgg gttacggcgg ctggcagcac
    17161 atcattttgc ctgcggtttc cattgccttt atgtcgctgg cgattaacgc acggctgctg
    17221 cgcgccagta tgctggacgt cgccggtcag cgtcacgtca cctgggcgcg tctgcgcggc
    17281 ctgaacgaca aacagaccga acgtcgccac atcctgcgca atgcctcgct gccgatgatc
    17341 accgccgtgg ggatgcacat tggcgaactg attggcggga cgatgattat cgaaaacatc
    17401 tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga tttttaaccg tgactatccg
    17461 gtgatccagt gctttacgct gatgatggtg gtggtttttg tggtctgtaa tttgattgtc
    17521 gatttgctca acgccgcgct ggacccgcgc attcgtcgtc atgaaggagc gcacgcgtga
    17581 actttttcat ctcttcccgc tggtcggtac gcctggcgct gatcattatc gccctgctgg
    17641 cgctgattgc gctcaccagc cagtggtggc tgccgtatga cccccaggcg attgatttgc
    17701 cgtcgcgcct gctttcgccg aatgcgcagc actggctggg caccgatcac ttaggtcgcg
    17761 atattttctc gcggctgatg gcagcgaccc gcgtgtcgct cggttcggta atggcctgcc
    17821 tgctgctggt gctgacatta gggctggtta ttggcggcag cgccgggctg attggcgggc
    17881 gcgttgatca ggctaccatg cgcgtcgccg atatgtttat gaccttcccg acctcgattc
    17941 tgtcgttctt tatggttggc gtgctcggca ctgggctgac caacgtaatt atcgccatcg
    18001 ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag cctggtgatt tcactgcgcc
    18061 agcgcgagtt tgtgctggca tcacggcttt ctggcgcggg ccatgtgcga gtgtttgttg
    18121 atcatctggc aggtgcggtg atcccttcgc tgctggtgct ggcaacgctg gatatcggcc
    18181 atatgatgct gcacgtcgcg gggatgtcct tcctcggcct cggcgtgacc gcgccgaccg
    18241 ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat ctggacccag ccgctgcaaa
    18301 tgttctggcc ggggctggcg ctgtttatca gcgtgatggc ctttaacctg gtgggtgacg
    18361 cactgcgcga tcatctggac cctcatctgg tgacggagca cgcacactaa tgccgcaaca
    18421 gattgaacta cgtaatatcg cgctacaggc cgcgcagccg ctggtgcacg gcgtatcgtt
    18481 aaccctgaaa cgcgggcgtg tgctggcgtt agtcggcggt agcggcagcg ggaaatcatt
    18541 aacctgcgcc gcggcgctgg gaattctgcc cgcaggcgtt cgccagacgg cgggggaaat
    18601 tttagccgat ggcaaaccgg tttctccctg cgccctgcgc ggcatcaaaa ttgccaccat
    18661 catgcagaac ccgcgcagcg cctttaatcc gctacgcacc atgcacaccc acgcgcgtga
    18721 aacctgcctg gcgctgggga aacccgccga tgacgctacg cttaccgctg ccatagaagc
    18781 ggtggggctg gaaaacgccg cacgcgtgct gaagctgtac ccgttcgaga tgagcggcgg
    18841 catgttgcag cgcatgatga ttgcgatggc ggtgctttgc gaatcaccgt ttatcatcgc
    18901 cgatgaacca accaccgacc tcgacgtggt agcacaggcg cgcatcctcg atctgctgga
    18961 aagcattatg caaaaacagg caccggggat gctgctggtc acccatgata tgggcgtagt
    19021 ggcgcgtctg gcggatgacg tggcggtgat gtcgcacggt aagattgtcg aacagggcga
    19081 tgtcgaaacg ctgtttaacg cccccaaaca tacggtgacg cgcagcctgg tttccgctca
    19141 tctcgccctc tacggtatgg agctggcatc atgactttac ttaacgtttc cggcctttcc
    19201 catcactatg cgcacggtgg atttagcgga aaacatcagc accaggcggt gctgaataac
    19261 gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc tggggcgcag cggctgcggg
    19321 aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc cctcacaagg gaatattagc
    19381 tggcgcggcg aatcgttggc aaagctcaat cgcgcccagc gtaaagcgtt ccgccgcgat
    19441 attcagatgg tatttcagga ctccatcagc gccgtgaatc cgcgcaaaac cgtgcgcgag
    19501 atcctgcgag aaccgatgcg tcacctgcta tcactgaaaa aatccgaaca actggagcgc
    19561 gccagcgaaa tgctgaaggc ggtcgatctc gacgacagcg tgctcaacaa gcgcccaccg
    19621 cagttaagcg gcggccagct ccagcgcgtc tgcctggctc gcgcgctggc ggtcgaaccg
    19681 aaactgctga ttctggatga agcagtttct aaccttgatc tcgtgttaca ggcgggcgtc
    19741 attcgcctgc tgaaaaagct acaacaacag tttggcaccg cctgcctgtt catcacccac
    19801 gacttacgcc tggtggaacg cttttgccag cgagtgatgg tgatggacaa cggacaaatc
    19861 gtcgaaaccc aggcggtggg agataaatta accttttcct ctgacgccgg acgtgtgcta
    19921 caaaacgcgg tattacccgc attccccgtg cgccgtcgca cctcagaaaa ggtttaacgc
    19981 aaatgcaacg agtcaccatc acgcttgatg acgatttact ggagacgctg gacagcctga
    20041 gccagcgtcg tggttataac aaccgttccg aagctatccg cgatattctg cgtagcgccc
    20101 tggcgcagga agctacccag cagcacggca cacaaggttt cgcggtgctg tcgtatgtgt
    20161 atgaacacga aaaacgcgac ttagccagcc gtattgtttc cacccagcat catcaccacg
    20221 acctctccgt cgccacgcta catgtgcaca tcaaccacga cgactgtctg gaaatcgccg
    20281 tgttgaaagg tgacatgggt gacgtgcagc attttgccga tgacgttatc gcccagcgcg
    20341 gcgtgcggca cgggcatttg cagtgcttgc cgaaggaaga ttgagtctat tattgggtcc
    20401 tttggtgaac gatcaacaaa gggccactag cacacctgat tcctctctaa ataccctatc
    20461 cgaaccttct cttttgtaac gttctaaata tattcctaaa aatcttcaat tcattgtgac
    20521 cacaagtttt tcttcgcttt ttcgtatgaa gatactgtca ttaaaataat agaaaaggat
    20581 ttta
//
LOCUS       DAZKFU020000069        20008 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZKFU020000069 DAZKFU020000000
VERSION     DAZKFU020000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.069"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            278..1621
                     /locus_tag="ACHOQQ_RS09935"
                     /old_locus_tag="pgaptmp_001987"
     CDS             278..1621
                     /locus_tag="ACHOQQ_RS09935"
                     /old_locus_tag="pgaptmp_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470245.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVTPLVPEEVHLLAVAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1770..3278)
                     /gene="putP"
                     /locus_tag="ACHOQQ_RS09940"
                     /old_locus_tag="pgaptmp_001988"
     CDS             complement(1770..3278)
                     /gene="putP"
                     /locus_tag="ACHOQQ_RS09940"
                     /old_locus_tag="pgaptmp_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGU4470246.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3437..3646)
                     /locus_tag="ACHOQQ_RS09945"
                     /old_locus_tag="pgaptmp_001989"
     CDS             complement(3437..3646)
                     /locus_tag="ACHOQQ_RS09945"
                     /old_locus_tag="pgaptmp_001989"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470247.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3701..7663
                     /gene="putA"
                     /locus_tag="ACHOQQ_RS09950"
                     /old_locus_tag="pgaptmp_001990"
     CDS             3701..7663
                     /gene="putA"
                     /locus_tag="ACHOQQ_RS09950"
                     /old_locus_tag="pgaptmp_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4470248.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQTVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVELALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENNEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7703..8341)
                     /gene="rutR"
                     /locus_tag="ACHOQQ_RS09955"
                     /old_locus_tag="pgaptmp_001991"
     CDS             complement(7703..8341)
                     /gene="rutR"
                     /locus_tag="ACHOQQ_RS09955"
                     /old_locus_tag="pgaptmp_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGU4470249.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8629..9720
                     /gene="rutA"
                     /locus_tag="ACHOQQ_RS09960"
                     /old_locus_tag="pgaptmp_001992"
     CDS             8629..9720
                     /gene="rutA"
                     /locus_tag="ACHOQQ_RS09960"
                     /old_locus_tag="pgaptmp_001992"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGU4470250.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGYFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9720..10412
                     /gene="rutB"
                     /locus_tag="ACHOQQ_RS09965"
                     /old_locus_tag="pgaptmp_001993"
     CDS             9720..10412
                     /gene="rutB"
                     /locus_tag="ACHOQQ_RS09965"
                     /old_locus_tag="pgaptmp_001993"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGU4470251.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10424..10810
                     /gene="rutC"
                     /locus_tag="ACHOQQ_RS09970"
                     /old_locus_tag="pgaptmp_001994"
     CDS             10424..10810
                     /gene="rutC"
                     /locus_tag="ACHOQQ_RS09970"
                     /old_locus_tag="pgaptmp_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HGU4470252.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10818..11618
                     /gene="rutD"
                     /locus_tag="ACHOQQ_RS09975"
                     /old_locus_tag="pgaptmp_001995"
     CDS             10818..11618
                     /gene="rutD"
                     /locus_tag="ACHOQQ_RS09975"
                     /old_locus_tag="pgaptmp_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGU4470253.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11628..12218
                     /gene="rutE"
                     /locus_tag="ACHOQQ_RS09980"
                     /old_locus_tag="pgaptmp_001996"
     CDS             11628..12218
                     /gene="rutE"
                     /locus_tag="ACHOQQ_RS09980"
                     /old_locus_tag="pgaptmp_001996"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGU4470254.1"
                     /translation="MNEAVSPGALSTLFTNARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12229..12723
                     /gene="rutF"
                     /locus_tag="ACHOQQ_RS09985"
                     /old_locus_tag="pgaptmp_001997"
     CDS             12229..12723
                     /gene="rutF"
                     /locus_tag="ACHOQQ_RS09985"
                     /old_locus_tag="pgaptmp_001997"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGU4470255.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            12744..14072
                     /gene="rutG"
                     /locus_tag="ACHOQQ_RS09990"
                     /old_locus_tag="pgaptmp_001998"
     CDS             12744..14072
                     /gene="rutG"
                     /locus_tag="ACHOQQ_RS09990"
                     /old_locus_tag="pgaptmp_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGU4470256.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14155..14328)
                     /gene="ymdF"
                     /locus_tag="ACHOQQ_RS09995"
                     /old_locus_tag="pgaptmp_001999"
     CDS             complement(14155..14328)
                     /gene="ymdF"
                     /locus_tag="ACHOQQ_RS09995"
                     /old_locus_tag="pgaptmp_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4470257.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            15097..15170
                     /locus_tag="ACHOQQ_RS10000"
                     /old_locus_tag="pgaptmp_002000"
                     /pseudo
     CDS             15097..15170
                     /locus_tag="ACHOQQ_RS10000"
                     /old_locus_tag="pgaptmp_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813257.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(15260..>20008)
                     /locus_tag="ACHOQQ_RS10005"
                     /old_locus_tag="pgaptmp_002001"
     CDS             complement(15260..>20008)
                     /locus_tag="ACHOQQ_RS10005"
                     /old_locus_tag="pgaptmp_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HGU4470258.1"
                     /translation="YVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGA
                     RGKGKAAAHYEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYM
                     TEAQRAGRVFKDGRYVDAEYPVRQEVYDAFKGVMKAINSSDMPRRSALLDEVRSKPYW
                     STDVEMAARAFERYVQDKARTAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRE
                     AFDHLFSTLKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARG
                     VMSGIKDSDLKVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRT
                     VREKLRHEIIHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPE
                     VQAGEFLAHMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLG
                     QRVREGYTPREDGAGASSQYSRSGKPDPFKVPEGEGERYREWLGRAMRSKRSSDIQIE
                     LGRTPPVLRHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPEAVYQSA
                     THQNSLVILFNLRDRNGDPVISAVHLKAIKNGLEINKIASVYGTERGKKLSNMERQGL
                     TLYRREKLTPDEDLPRGLQLPVVEDSLGSLNKILYPEDIRKGPYYSRTSSLTPEETIA
                     SRFVRQMQDKFQVLKAVQENIRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQ
                     PLAKLLADYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRV
                     RNSGKQAQYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQNAYRYYVPLKGQDVDG
                     VVSLPRTGKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRNRKNEVGNAFLKL
                     VQDNPDKDYWQVFTDDKPDTMRAIAERVDPETGETRREVVERPVPMAMMADRYFTTKK
                     DGKTYYIKLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIR
                     DVQTAVMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQW
                     QKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDA
                     NGAVENALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASI
                     QGTANLVRTLGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDD
                     GVNWYDKVPSHVKERNLVIMKSMFGGKAGEYWSIPLPHGYNVFFLLGHTAEGVAAGDL
                     TASRAAGNVVGGILGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQI
                     YQENMPFGTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDVTPESLKYWID
                     YISGGTGRFISKTTDAAVKSLNGIDIPEQQAPFLGKISGEVMPYADQQKMYDRMTEVA
                     QYHAELKSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQ
                     QSAMVKSVERDMKIAVDRFNREYNKKVGVD"
BASE COUNT         4649 a         5506 c         5130 g         4723 t
ORIGIN      
        1 aagaaattac tatttaccgc gatagttcaa ggcgtaaaaa aaataataat attcatgaaa
       61 ctcatcccga tggcagcatt gtaaattcgc caataactgg cattcctcct gctttttacg
      121 ggtgtttcca ttaacaccag aatgagcgga ttgtttacta catcaagaac gcatccgaag
      181 tgtacatcct ggtgcgccga ctgaatcgcc cggagccatg cggatggcta cgacttaata
      241 cgaatcatta accctgtaag gatacggaga gcatgacatg agaccaatat ccaacctcaa
      301 tacgagcgca gcaacctata ttccaccgca acaactcccg agtagttgtc tggaaacttt
      361 gacgcttctg gtgcagatga ataattatat caaaagggat gcggactaca gcacgggcat
      421 ggctgttact ccactggttc ctgaagaggt tcacttgctg gctgttgcga tgacaaccga
      481 actacatcga catcagttcc aaccggtggt aagtgcaaat gatctgcaac ttcctgaacc
      541 gttcaccttc gatgttgccg gtttcaccat caccttcacg aagacgcaag agcatgacaa
      601 cacgggcaat ctcatgaaaa ttgcagtcag caagagcggt gtgtgcacca gtacaaatat
      661 tactcttgag ctgtttcact ccattgtcac aaccctgatg tcacgcagtc aatatggaac
      721 ctttgatctc tggtccgtac ggccaatact cacggatgaa agtcaaaaca gggtgcatga
      781 agcagctcgc tactccccgg cccagcaata cggaagagaa gaaacacact ttaccaacaa
      841 tggcatgcga gaatttggaa atatgtctcc gctggcgtgg cgtaacgatg tggaattaca
      901 gcaatcgtgt aatacaagca acatcccacc aaatgctgca aatgacgaca taaacaacac
      961 caggcagacc atcgaggacc agccagaggc ggacgagcca cagcaatatg ttaaattgac
     1021 tgtagacgac atgcgaaaat gggcggctat ggaccagcag gcgcgaaacg ctctacaggg
     1081 agtttcagga tggtgtacgc gcaatcattt taatatcaaa aatgccagaa attacctgac
     1141 cgatcacggc ctgaactacg cgggacaggt aaaggtaaac aggccgcatg aatatgccaa
     1201 attcaccctt gaacatatcc gccagtgggc agccttaaac aagcatgtgc gaaaaccggt
     1261 tggttattta gaaaagtggt gtaaggaaag aaacctcgcg ccaactactg ccaggaatta
     1321 cctgaagaac gacgggctta ccgccctggg tgagttgaag ctgacagggc cgcagaaatg
     1381 ggtcacgttc accttcggag acatcgtaca atgggcgaat atgacccagg aggagcgtaa
     1441 cagcgcaggg ggagccgaaa agtggagtaa aaaacgcggc ttccagtggt ctactgccag
     1501 atcctacctg aaatcgagcg gagtgaccaa gcaaggtgcg agaaaactgg cgtggttaaa
     1561 aaacagcggg aatatgtcga atccattcta tctcgcccaa ccaaccagcc gcaggacata
     1621 agctgaaggt accgatagcg gcaattttgt tcatcagtgc cgggtgggca aatagcaatt
     1681 gcccccgaac actccctgcc aggcaactta taccgcatgg cagggaacat tcgggttaaa
     1741 aagcaaaaga taaataatta ctcttcagct tagccttcct gcaaccgtga cggcggtgcc
     1801 gaatgatagt gcgcatcggc ctcggcaaag cgtttttgca tcgccgctga cggcgcttta
     1861 cccagcaaac taaacactac aatcccaata ctgccgaaga taaagcccgg aataatttcg
     1921 tacagtccca gccagccgaa ctgtttccag acgataaccg tcagcgcccc gatgatcatc
     1981 cccgccagcg caccgttgcg cgtcatgcgt gaccacatca ccgagaacag caccaccgga
     2041 ccaaacgccg cgccaaagcc tgcccacgcg tagctcacta agcccagcac gcggttttcc
     2101 gggtttgccg ccagcgcaat ggccaccagc gccaccacca gcaccatcac acgccctacc
     2161 cacaccagct ctttctggct ggcatgttta cgcagaaacg ctttatatag atcttcggta
     2221 atcgcactgg aacataccag cagctggcaa cttaaggttg acattaccgc cgccagaatt
     2281 gccgacagca gaatcccggc aatccacggg ttaaacagaa tttgcgccag ttcgataaac
     2341 acgcgttcag cgttctggtt taccgcacct gctaccgccg ggtgctcgtt aaagtaagcg
     2401 ataccaaaga agccgacagc caccgcccct gccaggcaga ggatcatcca ggtcatactg
     2461 atacgacgcg catggacgat gctgtggtga gaatccgccg ccataaaacg cgccaggatg
     2521 tgcggctgcc cgaagtaacc cagcccccat cccatcagcg agataatggc aacaaagttc
     2581 agccctttga gcatatccac gttttcgatg ctcttttgtt tgatcacttc cagagagtcg
     2641 ccaaagccac cgacactgat aatgacgata accggcgtca ggatcagggc aaaaatcatc
     2701 aggctggcct gtacagtatc agtccagctc accgcgagga aaccgccaat aaaggtgtaa
     2761 aggatcgtcg ccgcagctcc cgcccacaga gccgtttcgt agctcatgcc aaaggtactt
     2821 tcaaacagac gcgcgcctgc cacaatgccc gaagcacaat aaatggtgaa gaacagcaaa
     2881 ataaccagcg cggagataat gcgcaaaatg cggcttttat cttcaaagcg tccggtgaaa
     2941 taatccggca atgttaaggc gttattgtta tattcggtat gcacacgcaa ccgcccggcc
     3001 accagcttcc agttgatcca cgcgcctaat gtcaggccaa tggcgatcca actttcggaa
     3061 atcccggaaa gaaaaaccgc gcccggcaac cccattaaca gccagccgct catatccgat
     3121 gcacccgccg ataatgccgt cacgaatggc ccaaggctac gtccgcccag aatatagtcg
     3181 tcaaagtttt tcgttgatcg ccaggcgata aacccaatca atatcatgcc aaagatatag
     3241 acacaaaatg tcaccaacat cggtgtgcta atagccatct aaagtctcca aaaaattatt
     3301 atcggcaatg tcgaaacttg ccgttatatc tgccaccgga acggggtaac agagtttatg
     3361 ttttaccagg gcgaccgtat cctgccggaa gcgctagtta ttcacaatcg atttaacaca
     3421 ccatttacat taaattttag tgctcagcga cactattttt catcaggttg cactctctca
     3481 cattttttgc ggttgcacct ttcaaaaatg ttaactgccg cagagaaaaa gtctgagtta
     3541 tttttttaat ccctgtcata tcgatttctt ttattaacat ttcattcatt tttaagcttg
     3601 ctacgcaagt cacatttaac atggttgcac aaagttgcaa catcatggat atttcacgat
     3661 aacgttaagt tgcacctttc agaacaacag gagtaatggc atgggaacca ccaccatggg
     3721 ggttaagctg gacgacgcga cgcgtgagcg tattaagtct gccgcgacac gtatcgatcg
     3781 cacgccacac tggttaatta agcaggcgat tttttcttat ctcgaacaac tggaaaacag
     3841 cgatactctg ccagagctac ctgcgctgct ttctggcgct gccaatgaga gcgatgaagc
     3901 accgactccg gcagaggaac cacaccagcc attcctcgac tttgccgagc aaattttgcc
     3961 ccagtcggtt tcccgcgccg cgatcaccgc ggcctatcgc cgcccggaaa ccgaagcggt
     4021 ttctatgctg ctggaacaag cccgcctgcc gcagccagtt gctgaacagg cacacaaact
     4081 ggcgtatcaa ctggccgata aactgcgtaa tcaaaaaaat gccagtggcc gcgcaggtat
     4141 ggtccagggg ttattgcagg agttttcgct gtcatcgcag gaaggcgtgg cgctgatgtg
     4201 tctggcagaa gcgttgttgc gtattcccga caaagccacc cgcgacgcgt taattcgcga
     4261 caaaatcagc aacggtaact ggcagtcaca cattggtcgc agcccgtccc tgtttgttaa
     4321 tgccgccacc tgggggttgt tgtttactgg caaactggtt tccacccata acgaagccag
     4381 cctctcccgc tcgctgaacc gcattatcgg taaaagtggt gaaccgctga tccgcaaagg
     4441 tgtggatatg gcgatgcgcc tgatgggcga gcagttcgtc acgggcgaaa ccatcgcgga
     4501 agcgttagcc aatgcccgca agctggaaga gaaaggtttc cgttactctt acgatatgct
     4561 tggcgaagct gcactaactg ccgcagatgc acaagcgtat atggtttcct atcagcaggc
     4621 gattcacgcc attggtaaag cgtctaacgg tcgtggcatc tatgaaggtc cgggcatttc
     4681 aatcaagctg tcggcgctgc atccgcgtta cagccgcgcc cagtatgacc gggtaatgga
     4741 agagctttac ccacgtctga aatcactcac cctgctggcg cgtcagtacg atattggtat
     4801 caacattgac gccgaagagg ccgatcgtct ggagatctcc ctcgatctgc tggaaaaact
     4861 ctgtttcgag ccggaactgg caggctggaa cggcatcggc tttgttattc aggcttatca
     4921 aaaacgctgc ccactggtga ttgattacct gattgatctc gccacccgca gccgtcgccg
     4981 tctgatgatt cgcctggtaa aaggcgcgta ctgggatagt gaaatcaagc gtgcacagat
     5041 ggacggcctt gaaggttatc cggtttatac ccgcaaggtg tataccgacg tttcttatct
     5101 cgcctgtgcg aaaaagctgc tggcggtgcc gaatctaatc tacccgcagt tcgcgacgca
     5161 caacgcccat acgctggcgg cgatttatca actggcaggt caaaactact atccgggtca
     5221 gtacgagttc cagtgcctgc atggtatggg cgagccactg tatgagcagg tcaccggaaa
     5281 agttgccgac ggcaaactta accgtccgtg tcgtatttat gccccggttg gcacacatga
     5341 aacactgctg gcgtatctgg tgcgtcgcct gctggaaaac ggtgctaaca cctcgtttgt
     5401 taaccgtatt gccgacacct ctttgccatt ggatgaactg gtcgccgatc cggtcactgc
     5461 tgtagaaaaa ctggcgcaac aggaagggca aactggatta ccgcatccga aaattcccct
     5521 gccgcgcgat ctttatggtc acgggcgcga caactcggca gggctggatc tcgctaacga
     5581 acaccgtctg gcctcgctct cttctgccct gctcaatagt gcactgcaaa aatggcaggc
     5641 cttgccaatg ctggaacaaa cggtagcggc tggtgagatg tcgcccgtta ttaaccctgc
     5701 ggaaccgaaa gatattgtgg gctatgtgcg tgaagccacg ccgcgtgaag tagaactggc
     5761 gctggaaagt gcggttaata acgcgccaat ctggtttgcc acgcctccgg ctgaacgcgc
     5821 ggcgattttg catcgcgctg ccgtgctgat ggaaagccag atgcagcaac tgattggtat
     5881 tctggtgcgt gaggccggaa aaaccttcag taacgccatt gccgaagtgc gtgaagcggt
     5941 cgattttctc cactactacg ccggacaggt gcgggatgat ttcgctaacg aaacccatcg
     6001 tccattaggg cctgtggtat gtatcagccc gtggaacttc ccgctggcga ttttcaccgg
     6061 gcagattgct gccgcactgg cggcaggtaa cagcgtgctg gcaaaaccgg cagaacaaac
     6121 gccgctgatt gccgcgcaag ggatcgccat tttgctggaa gcgggtgtac cgccaggcgt
     6181 ggtgcagttg ctgccaggtc agggggaaac cgtgggcgcg caactgacgg gtgatgatcg
     6241 cgtgcgcggg gtgatgttta ccggttcaac cgaagtcgct acgttactgc agcgcaatat
     6301 cgccagccgc ctggacgctc agggtcgccc tattccgctc atcgctgaaa ccggcggcat
     6361 gaacgcgatg attgtcgatt cttcagcact gaccgaacaa gtagtaatag atgtgctggc
     6421 ctcggcgttc gacagtgcgg gtcagcgttg ttcggcactg cgcgtgctgt gcctgcaaga
     6481 tgagattgcc gaccacacgt tgaaaatgct gcgcggcgca atggccgaat gtcggatggg
     6541 taatccgggt cgcctgacca ctgatatcgg tccggtgatt gatagcgaag cgaaagccaa
     6601 tatcgagcgt catattcaga ccatgcgtag caaaggccgt ccggtgttcc aggcggtacg
     6661 ggaaaacaac gaagatgccc gtgaatggca aagcggcacc tttgtcgccc cgacgctgat
     6721 cgaactggat gactttgccg aattgcaaaa agaggtcttt ggtccggtgc tgcatgtggt
     6781 gcgttacaac cgtaaccagc tgccggagct gatcgagcag attaacgctt ccggttatgg
     6841 tctgacgctt ggcgtccaca cgcgcattga tgaaaccatc gcccaggtca ctggctcggc
     6901 ccatgtcggt aacctgtacg ttaaccgtaa tatggtgggc gcagtggtgg gtgtgcagcc
     6961 attcggcggc gaaggattgt ccggcaccgg gccaaaagca ggcggtccgc tctatctcta
     7021 ccgtctactg gcgaatcgcc cggaaagtgc gctggcagtg acgctcgcgc gtcaggatgc
     7081 agagtatccg gtcgatgcgc agttgaaagc cgcattgact cagccgctaa atgcactgcg
     7141 ggaatgggca gcaaatcgcc cagaattgca ggcgttatgt acgcaatatg gcgagctggc
     7201 gcaggcagga acacaacgat tgctgccagg gccgacgggt gagcgcaaca cctggacgct
     7261 gctgccgcgt gagcgcgtat tgtgtatcgc ggatgatgaa caggatgcgc tgactcagct
     7321 cgccgccgtg ctggcggtgg gcagccaggt gctgtggcca gatgacgcgc tgcatcgtca
     7381 gttagtgaag gcattgccat cgactgtcag cgagcgtatt caactggcga aagcggaaaa
     7441 tattaccgct caaccgtttg atgcggtgat cttccacggt gattcggatc agcttcgcgc
     7501 attgtgtgaa gcagttgccg cacgggatgg cgcaattgtt tcggtgcagg gttttgcccg
     7561 tggcgaaagc aatatccttc tggaacggct gtatatcgag cgttcgctga gtgtgaatac
     7621 cgctgccgct ggcggtaacg ccagcttaat gacgatcggt taaacaggta gccggaggat
     7681 gttacacctt cctccggcat cattagcgtg gtcgaatccc ctcaataata atccgctgca
     7741 cgttttcaac cgtttgattg aaaaatacct catcgcgcaa cgttgcgcct gtcaccgcct
     7801 ccacctgagg ggcgaaatcg gcgtaatgtt gagtggaagc ccaaatcata aaaatcaaat
     7861 gctgcggatc aatcggcgcg agtttgccgc ttttgaccca accggcaatc agcgccgatt
     7921 tctcatcaat taatgccttc aaatcgcccg tcagttcatc cattaacagc ggcgcgcctg
     7981 ccagcatctc catacagaac aggcgcgaag cctgcggata atcgcgtgag acttccagct
     8041 tcagacggat gtactctttg atcgccgcca gcggggcgaa atcttcacga aacgctttta
     8101 acggtgccag ccagatatcg agaatctgcc gcagcacggc aatatacagc gcctcttttg
     8161 acggaaagta atacagcaga ttggtttttg aaacacccgc caactctgcg atctgctcca
     8221 gccttgtgcc gtgaaaaccg aattgtgaaa aagtgtccag tgctgcgcta agaatcgctt
     8281 ttttcttcgc actgactgcg cgcgaacgtt tacccgttgt tttcactgca ccttgcgtca
     8341 tgcgctctcc cctcttgtag attggcaaca ggatagcaaa acccccgccc ggcctcgacc
     8401 ttctgcactc tcatcgcgca cggatgcatg ttttatgtgc aactgttttg accgtttagt
     8461 ccacttttta ccagatattt tagataacgc aatgttaaca tattaataac attcactttt
     8521 taaaaactgg catccacttt gcaaacaagc caatacgcgg ctgaaaagga tatgcaggat
     8581 gcagcgcccc gtctgacttt cactttacga gacgaagaga ggttgatgat gaaaattggc
     8641 gtattcgtac ctattggcaa caacggctgg ctcatttcga cccacgcgcc gcagtacatg
     8701 ccgacctttg aactgaataa agccatcgtg caaaaagcgg agcactacca ttttgatttc
     8761 gccctgtcga tgatcaaact gcgtggcttt ggcggcaaaa ctgagttctg ggatcacaac
     8821 cttgagtcgt tcaccttgat ggcggggctg gcagccgtga cctcgcgcat tcagatttac
     8881 gccaccgccg ccaccttaac gttacctccg gcaatcgtcg cccgtatggc cgcaaccatc
     8941 gactccatct ctggcgggcg ttttggcgtc aacctcgtga ctggctggca aaagcccgag
     9001 tatgagcaga tgggtatctg gcctggcgat gactatttct cccgtcgtta cgactatctc
     9061 accgaatatg ttcaggtgtt gcgcgacctg tggggcacgg gaaaaagcga ttttaaaggc
     9121 tattttttca ccatgaatga ttgtcgcgtc agtccgcaac cgagtgtccc catgaaagtg
     9181 atctgcgccg ggcaaagcga cgctggcatg gcgttctccg ctcagtatgc cgatttcaac
     9241 ttctgtttcg gcaaaggcgt aaatacacct acggctttcg ccccgaccgc tgtgcggatg
     9301 aaacaggccg cagagcaaac cgggcgcgac gttggctctt atgtgttgtt tatggtgatt
     9361 gccgatgaaa ccgacgatgc cgctcgtgcc aaatgggaac actacaaagc gggcgcggat
     9421 gaagaggcgt taagctggct aaccgaacaa agtcagaaag atacccgctc cggtactgac
     9481 accaacgtcc gtcagatggc agatcccact tcggcggtaa acatcaatat ggggacgtta
     9541 gtcggttctt acgccagtgt cgcgcgcatg ttagatgaag tcgcaagcgt gcctggtgcc
     9601 gaaggcgtgc tgttaacctt cgacgatttt ctgtcgggaa tcgaaacctt cggcgagcgc
     9661 attcaaccac tgatgcagtg ccgcgcccat ctccctgtgc tgactcagga ggtggcatga
     9721 tgacgacatt aaccgctcga ccggaagcca ttaccttcga tccgcagcaa agtgcgctga
     9781 tcgtggtgga tatgcaaaac gcctatgcca cgccaggcgg ctacttagat ctcgccgggt
     9841 ttgatgtctc aaccactcgc ccggtcattg ccaacattca aaccgccgtg accgcagcgc
     9901 gagcggcagg gatgttgatc atctggtttc aaaatggctg ggatgaacag tatgtcgagg
     9961 ctggcggccc cggctcaccg aattttcata aatcgaacgc cttgaaaacc atgcgtaagc
    10021 agccgcagct acaggggaaa ttgctggcga aaggctcctg ggattatcaa ctggtggatg
    10081 aactggtgcc gcagcctggc gatattgtgc tgccgaagcc gcgctacagc ggtttcttca
    10141 atacgccgct ggacagcatt ttgcgcagcc gcggaatacg ccatctggtt ttcaccggca
    10201 tcgctaccaa cgtctgcgtc gaatcgacgc tacgcgacgg cttttttctg gagtatttcg
    10261 gcgtggtgct ggaagacgca acacaccagg cggggccgga atttgtacag aaagccgcgt
    10321 tgttcaatat cgaaaccttt tttggctggg tcagcgacgt cgaaacgttc tgcgacgcgc
    10381 tttctcccac gtcctttgct cgtatcgctt aaggagttta acgatgccaa aatccgtaat
    10441 tattcccgct ggcagcagcg caccgctggc ccccttcgtt cccggcacgc tggctgatgg
    10501 cgtggtgtat gtctccggta cgctggcttt tgatcaacac aataacgtgc tgtttgccga
    10561 tgacccaaag gcgcaaaccc gccacgttct ggaaactatc cgcaaggtga tcgagacagc
    10621 gggtggcacg atggcggatg tgaccttcaa cagcatcttt attaccgact ggaaaaatta
    10681 cgccgcgatt aacgaaatct acgccgagtt ttttccaggt gataaaccgg cgcgattctg
    10741 cattcagtgc ggactggtaa aacctgacgc gctggtggaa atcgccacaa ttgcgcatat
    10801 cgccaagtga ggccgcgatg aaactttcac tctcacctcc cccttatgct gatgcacccg
    10861 tagtggtgtt gatttcgggt cttggcggta gcggcagtta ctggttaccg caactggcgg
    10921 tgctggagca ggagtatcag gtagtctgtt acgaccagcg cggcaccggc aataatcccg
    10981 acacactggc agaagattac agtatcgccc agatggcagc ggaactgcat caggcgctgg
    11041 tagccgcagg gattgagcat tacgcggtgg tcggccatgc gctcggtgcg ctggtgggaa
    11101 tgcagctggc gctggattat cccgcgtcgg taactgtgct ggtcagcgtt aacggctggc
    11161 tacgaataaa cgcccatacg cgccgctgtt ttcaggttcg cgaacgatta ctgtatagcg
    11221 gcggcgcgca ggcatgggtg gaagcgcagc cgttgttcct ctatcccgcc gactggatgg
    11281 cggcccgcgc acctcgcctg gaggcagaag acgcgctggc actggcgcat tttcagggca
    11341 aaaataattt actgcgtcga cttaacgccc tcaaacgcgc tgactttagt caccatgcgg
    11401 atcgcatccg ctgcccggtg caaatcatct gcgccagtga tgatctgctg gtgccatcag
    11461 catgttccag tgaacttcat gccgccctgc ccgatagcca gaaaatggtg atgcgctatg
    11521 gcggacacgc ctgcaacgtg accgatcccg aaacgtttaa tgctctgtta ctcaacgggc
    11581 ttgccagcct gttacatcac cgtgaagccg ccctgtaagg aattgctatg aacgaagccg
    11641 ttagcccagg tgcgcttagc accctgttca ccaatgcccg cactcacaat ggctggcggg
    11701 agacacccgt cagcgatgag acgttacggg agctttatgc cctgatgaaa tgggggccga
    11761 catcagctaa ctgttctccg gcacgtatcg tgtttatccg cactgcagag ggaaaagaac
    11821 gtctgcgccc ggcgctttcc agcggcaatt tgcaaaaaac cctgaccgcg cccgtcaccg
    11881 ctatcgtcgc atgggacagt gaattttatg aacggttacc gcaactgttt cctcacggcg
    11941 atgcccgcag ttggtttacc tccagcccac aacttgccga agaaacagcg tttcgcaaca
    12001 gttccatgca ggcggcctat ctgatcgtcg cctgccgtgc gctggggctg gatactggcc
    12061 cgatgtcggg ttttgaccgt caacacgtgg acgacgcctt ttttgcgggt agcacgctga
    12121 agagcaatct gctgattaat atcggctatg gcgatagcag caaacttttt gcgcgcctgc
    12181 cacgtctgtc ctttgaagaa gcctgcgggc tgttgtaagg agcaaactat gaacattgtc
    12241 gatcaacaaa cttttcgcga tgcgatgtcc tgcatgggtg cggcggtcaa tatcatcacc
    12301 acggacggtc cagccgggcg cgccgggttc accgccagcg ccgtctgcag cgtgaccgat
    12361 acgcctccca cattactggt gtgcctgaat cgtggggcgt ccgtctggcc ggtattcaat
    12421 gaaaatcgaa cgctgtgtgt aaatacactt agcgccgggc aggagccgct ttcaaacctt
    12481 tttggcggca aaacgcccat ggaacaccgc tttgccgccg cccgctggca gactggtgtg
    12541 accggatgcc cgcaactgga agaggcgctg gtttcgtttg actgccgtat cagccaggtg
    12601 gtgagcgtcg gcacccacga cattttgttt tgcgccatcg aagcgattca tcgtcacacc
    12661 acaccctacg ggctggtgtg gttcgatcgc agttatcacg cgctgatgcg ccctgcttgt
    12721 taacctcctt aaggagacag ctgatggcaa tgttcggtct tcctcactgg cagttaaaat
    12781 cgacctctac agagagcggc gtggtcgcgc cggatgaacg attgccgttt gctcagacgg
    12841 caataatggg cgttcaacac gcggtggcga tgtttggcgc gacggtgtta atgccgattc
    12901 tgatggggct ggatcccaat ctttccattt taatgtcagg gatcggcacg ttactgtttt
    12961 tctttattac cggcgggcgc gtgcccagtt atctcggctc cagcgcggct tttgttggtg
    13021 tggtgatcgc ggcaacgggc tttaacggcc aggggattaa cccgaatatc agcatcgcgc
    13081 tgggcgggat tatcgcctgt gggctggttt ataccgtcat tggcctggtg gtgatgaaaa
    13141 tcggtacgcg ctggattgaa cggctaatgc cgccggtggt gacgggtgcg gtggtgatgg
    13201 cgattggcct gaacttagcg cctatcgcgg taaaaagtgt atcggcttca ggctttgata
    13261 gctggatggc ggtaatgacg gtgctgtgta ttgggctggt ggcggtgttt acccgtggca
    13321 tgatccagcg gttattgatt cttgtaggat taattgtcgc ctgtctgctg tatggcgtga
    13381 tgaccaacgt tttagggctg ggaaaagccg tcgattttac tctcgtcagc catgctgcct
    13441 ggttcggtct accgcatttt tccactcctg gttttaatgg tcaggcaatg atgctgattg
    13501 cgcccgtggc ggtgattctg gtggcggaaa acttaggcca tctcaaggcg gtcgccggga
    13561 tgaccggacg caatatggat ccgtatatgg gacgggcatt cgtcggtgat gggctggcga
    13621 cgatgctttc tggttctgtc ggtggcagcg gcgtcaccac ctatgccgag aatatcgggg
    13681 taatggccgt gacaaaagtc tactcgacgc tggtgtttgt cgctgctgcc gtcatcgcca
    13741 tgctattggg cttttcaccg aaatttggcg cgctgattca taccattccg gccgcggtga
    13801 ttggtggtgc atcaattgtt gtgttcgggc tgattgccgt cgcaggcgca aggatatggg
    13861 tacaaaaccg tgtcgattta agccagaacg gtaatttgat tatggtcgca gtaaccttag
    13921 tgctgggcgc gggtgatttt gctctcacgc tgggcggttt tacgttggga gggatcggta
    13981 cagcaacctt tggcgcgatt ttactcaatg cgttattaag cagaaggttg gttgacgttc
    14041 caccgccaga agttgttcat caggagccat aaacagaaaa ccgccgggat taccggcggt
    14101 gatgactttt tgacgagctt aagaaaaaaa tgcattcgtc attgattagc ccggctagtt
    14161 gtcgcttttg ccgtgactgc tcttgccacc ttttttacct gcttcagatg cgcgctgcgg
    14221 atcattcttg aaattacccc cgctgtgctg tccacctttt ttacctgctt ctgatgctct
    14281 ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta ctgacctccg
    14341 tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct gccatatttt
    14401 cgctacgtgg gactaagctt agtgaactga cgtgtttcta cccgcgtttg gtaatatcct
    14461 gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa gccgctctta
    14521 tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt gcgtgcttgt
    14581 gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa ggacgtctta
    14641 tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga gtggttagaa
    14701 atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac catgaagcat
    14761 taaaaacaca gaattaccga agatcgtcca agagcatata cttttcaata tgttagacct
    14821 cattttgacc ataaagaaat cgattttaca ccgttcttta tacgttcttt tttgccccaa
    14881 atatgcccca aacaaccaca ctttgcccca atagcgcccc aaagatttaa gggggtttaa
    14941 gtatatattt cagataatta acgcaagttt acgatgtgaa tttacgttaa ttttctgggt
    15001 agggtattta ccgtcattcg ttcatcaata tttctgcata ttgacaaata cgcctgagct
    15061 atcgcgggag aaatgcaatg aaagcagcaa aaggcgatgt taatcaccct gctcatctct
    15121 gtattaccgt cattgtgacg gcactgataa cgaagaaaga cctctgcaag ggaggaatcc
    15181 ctcgccacct ctgatgtgtc aggtatcctc accctcccca atcacattag ccccgcactt
    15241 ccgtacgggg ccatttctgt taatccactc ccactttttt gttgtactcg cggttaaacc
    15301 gatccacggc aattttcata tcccgctcta ccgatttcac catcgccgac tgttgcgcca
    15361 gactgagagt actgtcggcg taaatggcat cacgctgttt acgcaaatcc ttcagtctct
    15421 tccgggtatc ctgcataagc ccgttcatcg acaattttcc gttgttctcg tcaatgaacg
    15481 ccgttctttc tgcaccggtc agactcttca gctctgcgtg atactgcgca acctctgtca
    15541 tccggtcgta catcttctgc tggtcagcat acggcatcac ctcacctgaa attttcccca
    15601 ggaagggcgc ctgctgctcc ggtatatcaa taccgttcag cgatttcacc gccgcatccg
    15661 tggttttgga aatgaagcgc cctgtaccac cagagatata gtcaatccag tatttcagtg
    15721 attccggcgt gacatccacc gcgccgggac ggtactggct gccacctgag aacgcattca
    15781 gccaggatgc aaacgccttg tacgcttctg gcgttgaacg tcttcccagc tggctgtcag
    15841 gttttggtgt accaaacggc atgttctcct ggtaaatctg cgcccccatg aagttttcat
    15901 tcatggcaag gttcgcaaac ggacgcagaa tggtcggcgc tgcatttttc aacaatgccc
    15961 cggacagtgt ttccgacgtc tcactgccga tcgggctgaa tgcccccagg ataccaccga
    16021 caacattacc ggcagcacgg gacgccgtca ggtcacccgc cgccacacct tcggctgtgt
    16081 gaccaagcag gaagaaaacg ttgtacccgt gaggcagagg aatactccag tactctccgg
    16141 ccttgcctcc gaacatcgat ttcataatga cgaggttacg ctctttcaca tgagacggca
    16201 ctttgtcata ccagttaacc ccgtcatcat cctcccccgc aacactgcgg ttaagcgagc
    16261 caagcagata acccgctccc acagctgcaa gcgcgatttt ctgcggtaca ttgagattct
    16321 tccagcgaag gcgctccagt aacggcccgt cgccattaag atgtccgagc gttctcacca
    16381 ggtttgcggt cccctgaata ctggcgttgg cgaacatgta cagcgagttc atcagcgccc
    16441 cctgctcacc acgacggtta aagttcaccg tcatgttttt ggcaagagac gccgcctgct
    16501 ggcgtgacaa accggcatca cgggcgtgct tataggcaga aagacgcagt gcgttttcaa
    16561 ccgcaccgtt ggcatcctcc acaaggttca ggaacgaatt ccatgcaccg atactctggc
    16621 ctttccatcc ccccttcgcc agcgatacaa ggcgatccat ttccttctgc tggccttcaa
    16681 ggtcacccat gttaaaccag ccggttttac ctccatcctc aacaaactct ttccacacct
    16741 tctgccactg tgcgcctttg cccgtgaggg ttttaccccg cagactggcg tatacggctg
    16801 acatggcgga acggctgtct ttcaccacag ccagggcgga taagttatcc agccctttca
    16861 gtttgccgtc gctccttccc tgctcagcct tcaggttcat caccgccgtc tgcacgtcac
    16921 ggatgaagtt actgaccaga aattccgggt tatacgacgt gttcaccgtt gccaggaaac
    16981 ggttaacttt ccccagcgta cggattacgg cgttactggt ttccggcccc atgttcttca
    17041 tcgcacgcat caggcgcggg tcatggagtt tgatgtagta agttttgccg tcctttttgg
    17101 tggtgaagta gcgatccgcc atcattgcca taggcaccgg acgttcaaca acttcgcgac
    17161 gggtttcgcc ggtttccgga tcaacacgct ctgcaatcgc ccgcatggtg tccggtttgt
    17221 catcagtgaa tacctgccag taatccttat cgggattatc ctgcaccagt ttcaggaagg
    17281 cgttacccac ttcgtttttg cgattgcgaa tcagcgattc gctcaaatcc tgtatcgcct
    17341 gagtggaagg agactgtgcg cgggatgcac gccccatggc ctgcctgctt tcacgtccgc
    17401 cgatggtgaa gcccttacct gtacggggca gtgacaccac accgtcaaca tcctgacctt
    17461 tcaggggaac gtagtaacgg taggcgttct gccaggcatc caccacaccg ctctcttcca
    17521 gtccggcctc acggataagc tcacggcgac gggccagcat atcgtcaaca atccctgcca
    17581 gacggtcata ctgtgcctgt tttccactgt tacgcacacg ctgcatgatt tccgccgctt
    17641 ccgcgttggt catccccgaa ccgccgtccg gcattttcgg gttgattttc gcgatatgcg
    17701 cgttacgttc cggcgcgtga cgggcgtaga ggtactcatc cagatcggcc tgcgcaattt
    17761 tgtagtccgc cagtaattta gccagcggct gaacatagcg ctccttcatc acgttcaggt
    17821 cgttttccgc cttcccatgg aagagttctt ccgccatata agcgttgtta ctgtcgtcca
    17881 ctttgccgcc cgttttacgg atattctcct gcacagcctt cagcacctgg aatttatcct
    17941 gcatctggcg aacaaaacgc gatgcgattg tctcttccgg cgtcaggctg ctggtgcggg
    18001 agtaatacgg ccccttgcga atatcttcag gatagagtat tttattcaaa gaccccagtg
    18061 agtcttccac cacgggcaat tggagccccc ggggaaggtc ctcatctggg gttaattttt
    18121 ctcttctata cagggttaag ccctggcgct ccatgttgct taactttttc cctctttcgg
    18181 ttccatatac agaagctatt ttgtttatct ccagcccatt tttaatggcc tttaagtgca
    18241 ctgcacttat tactggatca ccatttctgt ctctgagatt aaacagaata acaagagagt
    18301 tttgatgagt tgcggactga taaacggctt ctggatcatg catcagttcc ggtagtctct
    18361 cgataacatc cattggcacc acatgtttca cgccattggt ggccttacgc acagtgtcgc
    18421 gggaaataac cagcggcaag tccggcgcac caagatgacg caatacagga ggtgtgcgtc
    18481 ctagctcaat ctgaatatca gatgaacgtt tggagcgcat ggctcttccc aaccattcac
    18541 gataacgctc accctcaccc tccggtactt taaacggatc aggtttaccg ctacgggagt
    18601 actgagacga tgcgccagca ccatcctcac gcggcgtgta accttcccgc acacgctggc
    18661 ctaacgtacg gatggtctcg cgaacaagtc tgatatcgtt cagttccgtc ggcttcagta
    18721 accccgtgcg acgcagtacc cctttgacca gggcaacaac acgctcccat gccgccacaa
    18781 atttattagg ctgcttctct gccatatgtg ccagaaattc acccgcctgc acttccggtg
    18841 attccttacc ataagacgca tcaaccttac gccaggcttc acggatggtg gcgttatcac
    18901 tgtcacgggt tttcagcacg gtcttaataa tcgtctgata ttccgctggc gtgacaacat
    18961 gctccatagc atggtgaatt atctcgtgac gcaacttctc gcgaacggtc cgcccgtcag
    19021 ggatgttatc cgccaccagg acaatttcac gtttatccgg acgatagaac gcgtgcaccc
    19081 tgccgtaacc gtcgaatgat tcccccgcca gcgcttcagc ctctttctgt gacttcacca
    19141 cacggacctt caggtcactg tccttaatgc cactcatcac gccacgggca accgcttcaa
    19201 cctgcgggac cgggctgcct ttggcttccg cactgcggtt aacatccgaa atgagattgc
    19261 cttcaggtgt gcgggtcacg cccttacggg aataaaacgc aacgcccttg tccgtctcac
    19321 gggttttcag cgtactgaac aggtgatcga atgcctcacg aatgccgcca tccagttccg
    19381 cattcgtcgg ataagcccag gtgttatctg tgttgtgctc aggtgcctta cggatattga
    19441 ccagataatc attctccacg ccagccgtac gcgctttatc ctgaacataa cgctcaaagg
    19501 cacgcgccgc catttcaaca tccgttgacc agtacggttt tgagcgcacc tcatcgagaa
    19561 gcgctgaacg acgcggcatg tcactgctgt tgatcgcttt catcacgcct ttaaacgcgt
    19621 cgtaaacctc ctgacgtacc ggatattccg catcaacata tctgccgtct ttaaatacgc
    19681 gcccggcacg ttgtgcttcc gtcatatagt cgccacctga cgtgatcttc ccgtcagtgg
    19741 aaacatcata acgaccaaaa taattatcca gtgaatggaa ccattcgtgg gccagcgccc
    19801 ccggtccgtt accttttgtc aggttgattg ccacctcacc cggctcatag tgtgccgccg
    19861 ccttaccctt accacgggca ccaaatgcca ggccaagacg accgttcagg gaaagcgctt
    19921 ttgtcggtac attcagtact tccgcaaggt catgcagcga gtcataagcc cggttcaaat
    19981 cagcctgacg acgcggacct tccacgta
//
LOCUS       DAZKFU020000070        19725 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZKFU020000070 DAZKFU020000000
VERSION     DAZKFU020000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.070"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            96..2276
                     /gene="katG"
                     /locus_tag="ACHOQQ_RS10010"
                     /old_locus_tag="pgaptmp_002002"
     CDS             96..2276
                     /gene="katG"
                     /locus_tag="ACHOQQ_RS10010"
                     /old_locus_tag="pgaptmp_002002"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGU4470259.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            2370..3275
                     /gene="yijE"
                     /locus_tag="ACHOQQ_RS10015"
                     /old_locus_tag="pgaptmp_002003"
     CDS             2370..3275
                     /gene="yijE"
                     /locus_tag="ACHOQQ_RS10015"
                     /old_locus_tag="pgaptmp_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGU4470260.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(3302..3919)
                     /locus_tag="ACHOQQ_RS10020"
                     /old_locus_tag="pgaptmp_002004"
     CDS             complement(3302..3919)
                     /locus_tag="ACHOQQ_RS10020"
                     /old_locus_tag="pgaptmp_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGU4470261.1"
                     /translation="MKASLVLVSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIEDGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(4193..5296)
                     /gene="gldA"
                     /locus_tag="ACHOQQ_RS10025"
                     /old_locus_tag="pgaptmp_002005"
     CDS             complement(4193..5296)
                     /gene="gldA"
                     /locus_tag="ACHOQQ_RS10025"
                     /old_locus_tag="pgaptmp_002005"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGU4470262.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(5307..5969)
                     /gene="fsa"
                     /locus_tag="ACHOQQ_RS10030"
                     /old_locus_tag="pgaptmp_002006"
     CDS             complement(5307..5969)
                     /gene="fsa"
                     /locus_tag="ACHOQQ_RS10030"
                     /old_locus_tag="pgaptmp_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4470263.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDKGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(5981..8482)
                     /gene="ptsP"
                     /locus_tag="ACHOQQ_RS10035"
                     /old_locus_tag="pgaptmp_002007"
     CDS             complement(5981..8482)
                     /gene="ptsP"
                     /locus_tag="ACHOQQ_RS10035"
                     /old_locus_tag="pgaptmp_002007"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4470264.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            8791..9870
                     /locus_tag="ACHOQQ_RS10040"
                     /old_locus_tag="pgaptmp_002008"
     CDS             8791..9870
                     /locus_tag="ACHOQQ_RS10040"
                     /old_locus_tag="pgaptmp_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGU4470265.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAICVNVL
                     KSLARKKGSSTDEKEDDLDLDFEIN"
     gene            9885..10205
                     /locus_tag="ACHOQQ_RS10045"
                     /old_locus_tag="pgaptmp_002009"
     CDS             9885..10205
                     /locus_tag="ACHOQQ_RS10045"
                     /old_locus_tag="pgaptmp_002009"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4470266.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            10256..12553
                     /locus_tag="ACHOQQ_RS10050"
                     /old_locus_tag="pgaptmp_002010"
     CDS             10256..12553
                     /locus_tag="ACHOQQ_RS10050"
                     /old_locus_tag="pgaptmp_002010"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4470267.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAAGCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            12519..13397
                     /locus_tag="ACHOQQ_RS10055"
                     /old_locus_tag="pgaptmp_002011"
     CDS             12519..13397
                     /locus_tag="ACHOQQ_RS10055"
                     /old_locus_tag="pgaptmp_002011"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGU4470268.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            13399..13740
                     /locus_tag="ACHOQQ_RS10060"
                     /old_locus_tag="pgaptmp_002012"
     CDS             13399..13740
                     /locus_tag="ACHOQQ_RS10060"
                     /old_locus_tag="pgaptmp_002012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4470269.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGISIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(13727..14578)
                     /locus_tag="ACHOQQ_RS10065"
                     /old_locus_tag="pgaptmp_002013"
     CDS             complement(13727..14578)
                     /locus_tag="ACHOQQ_RS10065"
                     /old_locus_tag="pgaptmp_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4470270.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTAKLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(14793..16526)
                     /locus_tag="ACHOQQ_RS10070"
                     /old_locus_tag="pgaptmp_002014"
     CDS             complement(14793..16526)
                     /locus_tag="ACHOQQ_RS10070"
                     /old_locus_tag="pgaptmp_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4470271.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYIVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QNVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(16709..19360)
                     /gene="ppc"
                     /locus_tag="ACHOQQ_RS10075"
                     /old_locus_tag="pgaptmp_002015"
     CDS             complement(16709..19360)
                     /gene="ppc"
                     /locus_tag="ACHOQQ_RS10075"
                     /old_locus_tag="pgaptmp_002015"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGU4470272.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
BASE COUNT         4400 a         5056 c         5501 g         4768 t
ORIGIN      
        1 aagatctcaa ctatcgcatc cgtggattaa ttcaattata acttctctct aacgctgtgt
       61 atcgtaacgg taacactgta gaggggagca cattgatgag cacgtcagac gatatccata
      121 acacgacagc cactggcaaa tgcccgttcc atcagggcgg tcacgaccag agcgcggggg
      181 cgggcacaac cactcgcgac tggtggccaa atcaactccg tgttgacctg ttaaaccaac
      241 attctaatcg ttctaacccg ctgggtgagg actttgacta ccgcaaagaa ttcagcaaat
      301 tagattacta cggcctgaaa aaagatctga aagccctgct gacagaatct caaccgtggt
      361 ggccagccga ctggggcagc tacgccggtt tgtttattcg tatggcctgg cacggtgcgg
      421 gtacttaccg ttcaatcgat gggcgcggcg gcgcgggtcg tggtcagcaa cgttttgcac
      481 cgctgaactc ctggccggat aacgtaagcc tcgataaagc acgtcgcctg ttgtggccaa
      541 tcaaacagaa atatggtcag aaaatctcct gggccgacct gtttatcctc gcgggtaacg
      601 tggcgctgga aaactccggc ttccgtacct tcggttttgg tgccggtcgt gaagacgtct
      661 gggaaccgga tctggatgtt aactggggtg atgaaaaagc ctggctgact caccgtcatc
      721 cggaagcgct ggcgaaagca ccgctgggtg caaccgagat gggcctgatt tacgttaacc
      781 cggaaggccc ggatcacagc ggcgaaccgc tttctgcggc agcagctatc cgcgcgacct
      841 tcggcaacat gggtatgaac gacgaagaaa ccgtggcgct gattgcgggt ggtcatacgc
      901 tgggtaaaac ccacggtgct ggtccgacat caaatgtagg tcctgatcca gaagctgcac
      961 cgattgaaga acaaggttta ggttgggcga gcacttacgg cagcggcgtt ggcgcagatg
     1021 ccattacctc tggtctggaa gtagtctgga cccagacgcc gacccagtgg agcaactatt
     1081 tcttcgagaa cctgttcaag tatgagtggg tacagacccg cagcccggct ggcgcaatcc
     1141 agttcgaagc ggtagacgca ccggaaatta ttccggatcc gtttgatccg tcgaagaaac
     1201 gtaaaccgac gatgctggtg accgacctga cgctgcgctt tgatcctgag ttcgagaaga
     1261 tctctcgtcg tttcctcaac gatccgcagg cgttcaacga agcctttgcc cgtgcctggt
     1321 tcaaactgac gcacagggat atggggccga aatctcgcta catcgggccg gaagtgccga
     1381 aagaagatct gatctggcaa gatccgctgc cgcagccgat ctacaacccg accgagcagg
     1441 acattatcga tctgaaattc gcgattgcgg attctggtct gtctgttagt gagctggtat
     1501 cggtggcctg ggcttctgct tctaccttcc gtggtggcga caaacgcggt ggtgccaacg
     1561 gtgcgcgtct ggcattaatg ccgcagcgcg actgggatgt gaacgcagca gccgttcgtg
     1621 cgctgccagt tctggagaaa attcagaaag agtctggtaa agcctcgctg gcggatatca
     1681 tcgtgctggc aggtgtggtt ggcgttgaga aagccgcaag cgccgctggt ttgagcattc
     1741 atgtaccgtt tgcgccgggt cgcgttgatg cgcgtcagga tcagactgac attgagatgt
     1801 tcgaactgct ggagccaatt gctgacggtt tccgtaacta tcgcgctcgt ctggatgttt
     1861 ccaccaccga gtcactgctg attgataaag cccagcaact gaccctgacc gcgccggaaa
     1921 tgaccgcgct ggtgggcggg atgcgtgtac tgggtgccaa cttcgatggc agcaaaaacg
     1981 gcgtcttcac tgaccgcgtt ggcgtattga gcaatgactt cttcgtgaac ttgctggata
     2041 tgcgttacga gtggaaagcg accgacgaat cgaaagagct gttcgaaggc cgtgaccgcg
     2101 aaaccggcga agtgaaatac actgccagcc gtgcggatct ggtatttggt tctaactctg
     2161 tcctgcgtgc ggtggctgaa gtttacgcca gcagcgatgc ccacgagaag tttgttaaag
     2221 acttcgtggc ggcatgggtg aaagtgatga acctcgaccg tttcgacctg ctgtaatctg
     2281 accttcttca gcgactgcct ttcaggcagt cgctgaagtt tctttaccgg cgtatagtgt
     2341 ccacaggaaa actacacact ggatctctca tgtctgccgc aggaaagagc aacccactgg
     2401 caatcagtgg cctggttgtg ctcacactta tctggagtta tagctggatt ttcatgaagc
     2461 aagtcaccag ctacatcggt gccttcgact ttaccgcctt acgctgcatt ttcggcgctc
     2521 tcgttttatt catcgtcctt ttattacgtg gtcgcggaat gcgcccgacg ccgtttaaat
     2581 acaccttagc cattgcgctg ttacaaacct gtgggatggt tggcctggcg cagtgggcgc
     2641 tggtcagcgg cggagcggga aaggtggcga tcctgagcta taccatgccg ttctgggtgg
     2701 tgattttcgc cgcgttgttt ctcggtgaac gcctgcgacg tggacaatat ttcgcgattc
     2761 tgattgccgc tttcggctta tttttggtgt tgcagccgtg gcaactcgat ttctcttcga
     2821 tgaaaagtgc catgctggca atcctctccg gtgttagttg gggggcgagc gcgattgttg
     2881 ctaaacgtct gtatgcccgt catccgcgcg tggatttatt gtcgttaaca tcctggcaga
     2941 tgctctatgc ggcgctggtg atgagtgtgg tcgctttact ggtgccgcaa cgtgaaattg
     3001 actggcagcc cactgtgttc tgggctctgg cctacagtgc gattctggcg acggcgttgg
     3061 cgtggagctt atggctgttt gtgctgaaaa acttgcctgc cagtattgcc agcttaagca
     3121 cactggccgt tcccgtttgc ggcgtactct tttcctggtg gctgctcggt gagaatccgg
     3181 gggccgttga aggtagcggt attgtgctga ttgtgctggc actggcgctg gtgagccgta
     3241 agaaaaaaga agccgttagt gtaaaaagga tctgaatttt ttcttcatgt ggggcgatcg
     3301 cttatttaac aaaataacga taatgcccca ccatgcgcca gctaaacagc acatcttctt
     3361 cctgcgcacc tgcgccaatg ttatgtatca ccagcggcgt accgtcgcgg gcgaagccgt
     3421 ctgaaaccac cccaatatgt gccagcccgt tatctaatcg ccaggagaca atatcgcccg
     3481 cttgatagtc actggggttc ttgctgatgg ggcgtgtttt atcgtggcgg gtaaaccagg
     3541 tttccagatt aggcacccga cggtgatcga tgttgctgtc cgggcgcttt aactgccatt
     3601 tttgcgggta ctcggcaaaa ttcttcgcca tatcttcgtg caccagtttc tgcaaatcga
     3661 ccttctggct gcgcaatgca cggatcacca catcggaaca taccccgcgt tcttgcggga
     3721 catcaccgcc aggataggta agctgcacat acgccggatc gtaaaatagc gtgctgccga
     3781 tttgctgtct ggcaccgtct tcgatggcaa ggttggtatt ggcctggatt tgtaccacgg
     3841 ttggtggaac ggcgggagat tttaaggagt ggctggtaaa tgccgttagc aggctgacca
     3901 gcaccagtga agctttcatc tgtttcgtcc gtgaaaataa gagtcacaga ttcgaccttc
     3961 cgggataacg attgttctgt agagaaaatg tccagaagcg gataaaaaac tcctgaacaa
     4021 cgtaggagtt tcagattact ggcacagagt gccggatgcg gcgtgaacgc cttatctggc
     4081 ctaaaaagca cgcaaattca atatattgca gagattgcgt aggcctgata agcgtagcgc
     4141 atcaggcaat tttgcgttca aactcccgga caagccggga gtttggagta ggttattccc
     4201 actcttgcag gaaacgttgt ccatactggt cagctaccag cagtgcggcg taaacctgat
     4261 ctggcgtcgc gccgccaggc atgttgtgaa tggtttcacc ttctgcacat gccgcttctg
     4321 ccacaattcg cattttcgcc gggacatctt ctttaatatc cagttgagcg agagttattg
     4381 gcaaacctac cgcatggcta agggcagcta cggtttcgat ttcctccacc ggcgcatttt
     4441 ccagaaccag ctgcgtcagc gtaccaaatg ccactttttc accgtgataa tagtgatgcg
     4501 cgtccgggat agcggtcagg ccgttatgca ctgcgtgcgc cgcagccaga ccaccacttt
     4561 caaagcctac accgcttaaa taggtgttcg cttcaatcac gcgctccagc gccggagtca
     4621 ctacatgctg ttcggcagca agcatcgctt tttcgccttc ttccagcagg gtgttgtagc
     4681 acagttcagc cagtgccagc gcagcctggg tgcacttgcc gcccgccatg gtggtcgcgc
     4741 cgctacgaga gcaggcacgc gcttcaaacc aggttgccag cgcatcgccg atacccgccg
     4801 ctaacagacg tgcaggtgcg ccagcgacga ttttggtgtc gacaatgacc atattcgggt
     4861 tatttggcaa cagcagatag cggtcaaact caccctcatc ggtgtagata acagacaatg
     4921 cgctgcacgg tgcgtcggta gaggcgatag tcggtgcgat cgctaccgga acacccatga
     4981 aatgtgccag tgctttggca gtatcgagag tttttccgcc accgataccg agaattgcgc
     5041 cacactgcgc agtctccgcg atgccacgca gacggtcgat ctcattttgc gaacattcac
     5101 cgccaaacgg cgcaatttct actaccagtc cagcatcttt aaagcttttc tcgacagtgg
     5161 attgagcaaa acctaaaaca aatttgtcac ctaccactaa ccagcgttct gccagcggct
     5221 tcaggtattc gcccagacga ttaatcacat cagcgccctg gatgtattta cccggtgatt
     5281 gaataatgcg gtccataatt gctcctttag agatgagtag tgccaaatgc ggcattccag
     5341 tcgtgttcga acttctctat agctgactct accgcagggg tgttgagcat ttgttgcgct
     5401 acatctaagg gcagggtgat ggattcacat cctgccagta aacagtccag cgcctgacgc
     5461 ggcgttttaa agctggctgc cagcaccatg ctttctggcg cgtgcatttc taacagcgct
     5521 tgcagctcct gaaccgtacg aatgccgtct ccgccctggg catctacgcg gttaacatac
     5581 ggagcaacgt attttgcccc tgccagcgcg gccagtaacc cttgtgcagc actgtacact
     5641 gcggtgccaa gcgtagtaat accctctttt ttcagcatct taatggcggc cagaccttcg
     5701 gaagtcaccg ggattttcac cacaatgccc ggaatggcgt cgcgcagacg cttcgcttct
     5761 tccaccatcc cctgcgcgtc gcggctcatg gtctgagcaa acagaatgcc cttatcacca
     5821 atcgcttttt gcagacgcgg cagcacttcc cagatggact ccttgctggc agcgataatg
     5881 ctcgggttag ttgtcacccc ggcaatcggg aaaatgcgtg ccagacgttc gacttcagcg
     5941 acgttagcgg tgtccagata cagttccatg atgtttcctt ttacagttcc agttcatgtt
     6001 gcagcaggct ggcgatagcg tcggcagagg cggcgttaac cagcgcgtta cggaattctt
     6061 cgtgcatgat gcgacgagcg aggcgcgaga aaatgcgcat atgttgatcg cccgcagcgt
     6121 gtttgttcag ggttaacatg atgatgaatt gcgcttcatc atcgccccag cgcaccggcg
     6181 cttgcagacg cgctacgctg atggtggact gctcaatgtg ttctgatttg ctgtggggaa
     6241 tggcaaaact aaagcccaga ccggtagaga aaacggcctc gcgcgcccac aagtcggctt
     6301 ccagtttacg cggatagcgg cagcggcccg ccagcagcag gttatcggtc atgcctttga
     6361 gtacttcttc tttgctgcgc cagtcgcttt ccagcgtgat gcactcggcg gtgaccagtg
     6421 gtgcgtcctg ttgggtcatg cggaattgcg ccagcagatg ttccacttcc agcgaagtac
     6481 ggcaggccat tgcctggttg agcaactggc ggcactcacg gctatcaagt tgcgccattc
     6541 gcgctttcgc cgccggaatt gacggtgcgc tcatgctgag ttcatccagc cctaaaccga
     6601 ccagcaacgg cagcacggaa cctttcgctc ccagttcacc gcacagacca atccatttgc
     6661 cctggcgatg caccgcctgc acggcgtaat cgagcgcccg caagaatgcc ggattcaggc
     6721 tgttgtagtg acgagtaacc ttagcgttat cgcgatccac cgccagcaga tattgcgtca
     6781 ggtcattact accaatacta aagaaatcaa tctcttcgca gcattgatcg atgatgaaca
     6841 tcaccgacgg cacttccagc atgatgccga gctggatctt ctcatcaaac ggaatgtgtt
     6901 cgttacgcag ttgctgtttg gcttccgcca gtttttcttt cacccataag atctcttcca
     6961 ttgaagagat catcgggatc atgattttca ggctgccgtg agcggaggca cggagaatcg
     7021 accgtagttg tgtggtaaat aacgacgcgt actcttcata aatacgcacg gcgcgataac
     7081 caaggaacgg gtttgcctct gcgggaatgt tcagataatc aacgggtttg tcaccgccaa
     7141 tatccatagt gcgcacaata atgctgcgtc cgttggcgga ctccagcgcc tgacaaaaaa
     7201 tgttgtacag ctcgctttcg cccggtgcac tggtgcgatc catatagagc atttcagtgc
     7261 ggaacaaacc aacgccttcc gcaccattgc cgaatgcggc ctgcgcttcc acggagtgag
     7321 cgatgttagc ggcaatttca atgcggatac cgtcagcggt acgggcttgt tgggtcagcc
     7381 agacacgctg ttgctcacga agggcgtcct gtacgcgggc ttcttgctga taataacgag
     7441 ctacggcttc ccctggctca accacaatcg ccccggcgtt gccgtcgata taaatcgttt
     7501 gatgctgcca cggagtaagg gcatcaatat ctacaccaac cagtgtcgga atgttgaacg
     7561 aacgggcaag aattacggta tgtgaggtgg tgccgccgct tttcagtaac aatcctttga
     7621 ggtgattttt atccagttcg aggaactggc tgggggtcag ttcatcagcc atacaaatgg
     7681 cgggctgcgt cagtttgccc ggtgccggga agcgttgctc accgtagatt tgctggagta
     7741 actggaagca gacgtcgcgt acgtccaggg cacgttcttg caggtagctg ctgctggaac
     7801 gggcgaactc ttcgcaaaag tgattcgcgc tggcaacaat tgcttcggcg cagcttaatc
     7861 cggcgctgac gcctgccagt aaatgttcgt gcagggaagt atcgccagcc agggatcggt
     7921 gcgcttccag aatcgcgctg gtggcaccgt cgctatccag cagacgaaac tcaatgtttt
     7981 tcagtaccag cgtcaggccg ttttccagtg cggattgctc ggcgtcaacg tctttggctg
     8041 cgggaagatt acccagcgca ttgagatcta aagaagagat cggcgtcaga atgccgcctg
     8101 cgctaccgct gcacacggtg cgggcgcgga taatttgcgg attcagattg gtcagtgaaa
     8161 ccggcagtgg ttccagttcg tcagatttaa cttccgccag cggcgcatcg cagtggggga
     8221 attcatcgcg cagccattgg cttaaacgct ggtgcgcttc ctgttcgtcg gccccggaaa
     8281 tcagtaactg gcagttatcg cccgccagcg tgtcggtgcc aatcagcgca agggcgcttt
     8341 tggcgttgcc cttgcggtca gtgcgcaggt tatgccactc aatttgtgat gaaaaagtat
     8401 tacacagcgt ttcaacgtgg cttgccggac gtgcatgtac gccgttaggt agctcacaaa
     8461 taaattccac aatcagggcc attgcctctc tcccggtacg gttttctgtt tacaggataa
     8521 gagcgcacgg caacggcctg ccatgtgaca aatctgccaa aagctggaca aatgtaatgt
     8581 aaccgtcaat ttgcgacgcg tctcacaaga cgccgttttg cggcatgctt ccggattatc
     8641 gcaagttatg aggcggatcg catttttgta ctgatattac aaaagtccag taaatggcct
     8701 ttttatccac tgtttgctcc gcctgcgatt gcctattgtt tcctccatca aaaatatgga
     8761 catgggcttt cgcaagcccg ggagcagctt atgaatgagt tggtgcagat cctgaaaaat
     8821 acccgtcagc atttaatgac gggcgtttca cacatgattc ccttcgtggt atcgggcggt
     8881 attttgctgg cggtttccgt catgttgtat ggcaaaggcg cagtgccgga tgccgtagcc
     8941 gatccgaatc tgaaaaaact gtttgatatc ggcgttgcag gtttgacgct gatggtgcct
     9001 ttcctcgccg cttacatcgg ttactccatt gcagagcgtt ctgcgctggc tccgtgcgct
     9061 atcggtgcct gggttggtaa cagctttggt gcgggcttct ttggtgcact gatcgccggg
     9121 attatcggcg gcatcgtggt gcattacctg aagaaaattc cggtgcataa agttctgcgc
     9181 tcggtgatgc ctatcttcat cattcctatc atcggcacac tgattaccgc aggcatcatg
     9241 atgtggggct tgggcgagcc tgtaggggcg ttgaccaaca gcctgactca gtggcttcag
     9301 gggatgcagc agggcagcat tgttatgctg gcggtgatca tgggtctgat gctggcgttc
     9361 gatatgggcg gtccggttaa caaagtggcc tatgccttca tgctgatttg cgttgctcag
     9421 ggtgtttata ccgtggtggc cattgctgcc gtgggtattt gtatcccgcc gctggggatg
     9481 gggctggcga cgctgattgg tcgtaaaaat ttctccgcag aagagcgcga aaccggtaaa
     9541 gcggcgctgg tgatggggtg cgttggggtt actgaagggg cgattccttt cgccgctgcc
     9601 gatccgctgc gtgttattcc ttccatcatg gtcggttcag tttgtggtgc agtaactgcg
     9661 gcgctggtcg gtgcgcagtg ctatgcaggc tggggtggtc tgattgtact gccggtggtt
     9721 gaaggcaagc tgggttatat cgcagcagtg gctgtcgggg cagtggtgac ggctatttgt
     9781 gtgaacgtgc tgaaaagtct ggcgcgtaaa aaagggtctt cgactgatga gaaggaagac
     9841 gacctggatc tggattttga aattaattaa ttgaggaacg agccatgaca aaaattattg
     9901 cagtaaccgc atgtccatct ggtgttgccc atacctatat ggcggcagag gcgctggaaa
     9961 gtgccgcgaa agcgaaaggc tgggaagtga aagtagaaac ccagggctcg attggtctgg
    10021 aaaacgaact gactgcggaa gatgtggcga gcgccgatat ggtgattctg accaaagata
    10081 tcggcatcaa gtttgaagag cgttttgcgg gcaagaccat tgtgcgcgtc aacatcagcg
    10141 acgcggtaaa acgcgctgac gccattatga gcaaaattga agctcacctg gcgcaaaccg
    10201 cttaagttct tctcccgctc gcaagggcgg gttcgctttc ccacaggagt tcctcatgac
    10261 gaatcgtatc tctcgcctca aaactgcact gtttgccaat acccgtgaaa tctcgctgga
    10321 gcgtgcgctg ctttataccg ccagccatcg gcaaaccgaa ggcgaaccgg tgatattgcg
    10381 ccgggcgaaa gcaacagcgt atatccttga acatgttgaa atttcgattc gtgatgaaga
    10441 actgattgcc ggtaaccgca cggtgaaacc acgcgccggg attatgtcgc cggaaatgga
    10501 cccttactgg ctgctgaaag agctggatca attcccgacg cgtccgcagg accgctttgc
    10561 tatcagcgaa gaagataaac gtatctaccg cgaagagttg ttcccgtact gggaaaaacg
    10621 ttcgatgaaa gatttcatca acgggcagat gacggatgaa gtaaaagccg cgaccagcac
    10681 gcagattttc agcatcaacc agacggataa aggccagggg cacattatta ttgattaccc
    10741 gcgcctgctg aatcacgggc tgggggagct ggtggcgcag atgcagcaac attgtcagca
    10801 acagccggag aatcactttt atcaggcagc gctgttactg ctcgaagcct cgcagaaaca
    10861 cattttgcgt tacgccgaac tggcggaaac gatggcggca ggctgcactg atgcccagcg
    10921 tcgcgaagaa ctgctgacta ttgcggagat ctcccgccat aacgccgaac ataaaccgca
    10981 gacgttctgg caggcgtgcc agttattctg gtacatgaac atcattctgc aatacgaatc
    11041 caacgccagt tcgctgtctt tagggcgctt cgaccagtat atgttgccgt tctatcaggc
    11101 atctttaacc cagggcgaag atccggcgtt cctgaaagaa ctgctcgaat ctttatgggt
    11161 gaaatgcaac gacattgtgc tgttgcgctc caccagcagc gcgcgttatt tcgcaggttt
    11221 cccgactggc tataccgcac tgctcggcgg gttaaccgag aacggacgta gcgcggtgaa
    11281 cgtgctttcg ttcctctgcc tcgatgccta tcaaagcgta caattaccgc aaccgaacct
    11341 aggggtgcgc actaacgcct tgatcgacac gccgttcctg atgaaaaccg cagaaaccat
    11401 tcgcctcggc accggtattc cgcaaatctt taacgatgaa gtggtggtgc cagcgttcct
    11461 caaccgtggc gtttcgctgg aagatgcgcg cgactattcc gtagtgggct gtgtggaatt
    11521 atctattccc ggcagaacct acggcttgca tgacatcgcg atgtttaatc tgctgaaagt
    11581 gatggaaatc tgcctgcatg aaaatgaagg caatgctgcg ctgacttatg aaggtttact
    11641 ggagcagatc cgcgccaaga tcagccacta catcaccctg atggttgagg gcagcaatat
    11701 ttgtgatatc ggccatcgcg actgggcacc tgtaccgctg ctctcatcgt ttatcagcga
    11761 ttgtctggaa aaaggccgcg atattaccga tggcggcgcg cgttataact tctccggcgt
    11821 acagggaatt ggtatcgcca acctgagcga ttctctccat gcgttgaaag ggatggtttt
    11881 tgatcaacag cgtttaagtt ttgacgaatt gctgtcggta ttaaaagcca actttgcaac
    11941 gccagaaggc gaaaaagtcc gcgctcgctt aattaaccgc tttgagaaat acggtaacga
    12001 tatcgacgag gtggataaca ttagcgccga gctgttgcgc cactactgca aagaagtgga
    12061 aaaataccag aacccgcgcg gcggttactt cacgccggga tcgtataccg tttctgctca
    12121 cgtcccgttg ggatcggtgg ttggcgcgac gccagacggt cgttttgccg gagaacagct
    12181 ggcagacggc ggcttgtcac ccatgctggg ccaggacgca caagggccaa cagcggtact
    12241 gaagtcagtc agtaagctcg ataatacgct gctgtctaac ggtacgttgc tgaacgtgaa
    12301 attcactccg gcgaccctgg aaggtgaagc gggattacgc aaactggccg acttcttacg
    12361 ggcgtttacc cagcttaagt tacaacatat tcagtttaac gtggtgaacg ccgacacgtt
    12421 gcgggaagcg caacagcgcc cacaagatta tgccgggctg gtggtgcgcg ttgccggata
    12481 cagcgccttc tttgtcgaac tgtcgaagga gatccaggat gacatcatcc gccggacagc
    12541 gcatcagctg taacgttgtg gaaacgcgcc gcaatgatgt ggcgcgcatt ttcaacattc
    12601 agcgttattc actgaatgac ggcgagggca ttcgtacggt ggtctttttt aaaggctgtc
    12661 cacatctttg cccgtggtgt gctaatccgg agtcgatctc cggcaaaatc cagactgtac
    12721 gcagagaggc gaaatgtctg cactgtgcga aatgtttgcg tgatgcggat gaatgcccct
    12781 ccggggcgtt tgaacggatt ggtcgcgata tcagccttga cgcgctggaa cgggaagtga
    12841 tgaaagatga cattttcttt cgcacgtccg gcggcggcgt cacgctttct ggcggcgaag
    12901 tgttaatgca ggcggagttt gctacccgtt ttttacagcg actgcggctg tggggtgtgt
    12961 catgcgccat tgaaactgcc ggagacgcac cagccagcaa gctgttacca ctggcgaaat
    13021 tgtgcgatga agtgttgttc gatttaaaaa ttatggacgc gactcaggcg cgggatgtgg
    13081 tgaagatgaa cctgccacgc gtgctggaga atctgcgttt gctggtgagt gagggcgtca
    13141 acgtgatccc gcgtttaccg ctgatccctg gtttcacgct cagccgggag aatatgcagc
    13201 aggcgctgga cgtgctgatc ccgctgaata tcaggcagat ccatctgtta ccgtttcatc
    13261 agtacggcga accgaaatac cgcctgctgg ggaaaacatg gtcgatgaaa gaggtgcctg
    13321 cgccgtcgtc agctgatgtg gcaacgatgc gcgaaatggc agaacgggcc ggatttcagg
    13381 ttaccgtggg aggttaaaat ggcatacctg gtggcagtaa ccgcctgcgt aagcggcgtg
    13441 gcgcatactt atatggcggc ggaacggctg gaaaagttgt gccagttaga gaagtggggc
    13501 atcagcattg aaactcaggg cgcgctggga acggagaatc gtttagcgga cgaggatatc
    13561 cgtcgggcgg atgttgctct gttgattacg gatatcgagc ttgccggtgc cgagcgattt
    13621 gaacattgcc gctatgtgca atgcagcatc tacgcattcc tgcgtgagcc gcagcgggta
    13681 atgagcgcgg tgcgcaaagt gctttctgcg ccgcagcaaa cccatcttat tctggagtag
    13741 tcggtttttc tgtcagttgg ctgtggtact gccgacgata ttccgacggc gagcgttcgg
    13801 tgtttttacg aaacagacgg cagaagtagt tgctgtcgac aaagccgcag gcgtgcgcca
    13861 cttcttttac cttcaggtca tagcctttca ataacgtctt agcgtgctcc agtcgcgtgt
    13921 gattcaggta ttcgttaaaa ccaatggccc ccgttttttg aaacaggtgc gagagatagt
    13981 ttggtgaaat atagaatgcc tgtgcgacag attcgcgggt aagcgcggag gcatagcgtt
    14041 cgtcgatata atcacgaata gcttcgaaca gtgcctggct gcgtgaggcg gtctggattt
    14101 ggctgccgag caaatcgcgg cagtggctaa gcaggctggc gacgataagc tttgccgttt
    14161 gctgctcctg cggctgcatt tgcatttcat tgagcgtttg tagcagaaaa gaacctatgc
    14221 gtgggccgcg tcgggcgacg tgttgcttcg ccagattttg atattgtttg ccatcccatt
    14281 gcacgacgct gaagccgagt tgctgtttgc caaacagcac gctaaaggta gtggcggggg
    14341 cttgccattg tggaaaattc cagccaccag caggaacgta cagcacatcg ccgggaacta
    14401 acgttgctcc agcgccggta tcaacaaact caccttccag cacaatttcc agccgtggaa
    14461 aatcgacctg atacgcaaga tcgggcaccg ggccgttact gctggcaaag taaatctggc
    14521 gcagggacag cgggccgttg atcaggcggg agagcagata gctgacgtcg tgatacatgt
    14581 tattccctta ggtatgtaga cgggaaaaca gtaactggca ttattaaaaa aggccacctg
    14641 aaaagtgtgg cctgagggga gttcgatgta aacattgcgg cagttgtatt gccggatgtg
    14701 gcgtaaacgc cttatccagt ctaccgccgc gatctgtagg ccggataaga cgcgtcaagc
    14761 gtcgcatccg gcaaatagcg cctggctgat aattactgat tacctacctg gtcgccatat
    14821 ggcagcgtgt cgtaatcgat cagcgcgttt ttcttgtacg ggttaccaat ccagcgggta
    14881 gtttctttga actgcggatt caccactgaa cgggttggat cgtaaccgtc gtaagataag
    14941 cctgctaaat ctgaccaggt gtggatcagt tccgcaaggc tgtatttacg atcaacattc
    15001 tgcgagaaat cacgcggatg agtcgcttgc catttttctg acgtccacag caggaacgga
    15061 atggtgtaca tatgacgcgt cgggttgtcc tcattacgcc cctgggtttt atgaggcggt
    15121 gtgtcgtaaa cctcttcacc gtggtcagag aagtaaacca ggaatccgtt cgggtctgcc
    15181 gctttaaagt ctttaatcag gctggcaacc acatgatcgt tatacaggtt agcgttgtca
    15241 taatcgttat atgactccag ttcttctgcg cttaatcccg gtggaacatg atcggtattg
    15301 ccatcaaact tgccctgatt ttccgggtag cggtatttgt atttgatatg cgtacccagc
    15361 agatgaacaa tgatcagttt cttcggcgca gggtccttca gcacttcctg gaacggcttc
    15421 agcacgttag tgtcgtattc acgcgcactc tgcgtacgtt gctggttcat gtagtactgc
    15481 ttgtcggtct ggcgcgaaaa taccgtcagc atggtattgc gggcggtcat cgtctgctgg
    15541 ttggtgatcc agaaggtttt ataacccgcc tgtttcatca tgttcatcag cgacggctgc
    15601 gtcagataca gatcagggtt cttttcattg gcaaaggtca gcgcctgttg caggatttca
    15661 atggtgtacg gacgagacgt aactacgtta ttaaacacgg ttagattcgg atcggtttta
    15721 tgcagcgcat ccagctccgg cgtggtttca cgcggataac cgtacagact catgcgtcca
    15781 cgctgggttg actcgccaat caccagcact aaggtgcgcg gttcgttacc cgattcatct
    15841 ttgaaattag ccagcggcgg caaggcatta ttttcattca gtagctttgt tagcgagttt
    15901 agttgctgac gatactgata atagccggtc aggaattgcc acggtgcggc aggctccata
    15961 cgcgaggcca ggttatccaa cgttttctca aacggcttgt ttttgataaa cgtattcatg
    16021 gcgatcggat gcagaatcaa gccataaagc agggcaaaag agacaatata acgccacggc
    16081 tttggaatat agaccgggcg cagacgtgtc cacagcagaa ctgccactgc cgtataggcc
    16141 agcgcgataa gcacaatttt caggctgaaa tactggctta aatactcgct ggcttcgttg
    16201 gtgttggttt cgaacatcac aaacagaacg ctctgcgaga actcctgccc gtagatgacg
    16261 tagtagcaca gcgccgccag agaggccgcc catagcacca cgccgatcac tgcggcaata
    16321 attttgatcc gcttcggaaa gaggaatacc ggaattaacc acagcgagct gaataacagc
    16381 gagtcgcgaa tgccgttggt gccactataa ccactgatgt aaataatggc ctgtagcaga
    16441 gtagagaaaa accaaaagta gagcagtgcc caacccaggg ctttccagct aaaaagaggt
    16501 ttagcctgga cttctgtgga atgcatagta agaacctgtc ttgaaaaaaa tatcgccgaa
    16561 tgtaccgaca attccttaag gatatctgaa gggatatcca gaatttgaat aaaatacaga
    16621 cagaaatata ttgaaaacga gggtgttaga acagaagtat ttcagaaaac cctcgcgcaa
    16681 aagcacgagg gtttgcagga gaggaagatt agccggtatt acgcatacct gccgcaatcc
    16741 cggcaatagt gaccattaac gcctgctcga cgcgaggatc cggttcctgg ccttcttttt
    16801 ctgcctggcg ggagcggtgc agcaactcgg cttgcaatac gttcagcggg tcggtgtaaa
    16861 tattccgtag ctgaatagac tctgcaatcc acggcagatc ggccatcaga tgggaatcgt
    16921 tcgcaatcgc cagcaccact ttgatgtctt cttcttgcag attgcgtaac tctttaccta
    16981 acggccacag tgctttgtct accaggcgtt ggtcatagta ttccgccagc cacagatctg
    17041 ctttggcgaa taccatttcc agcatgccga gacgcgtcga gaagaatggc caatcgcggc
    17101 acatagcctc cagctcgctc tgtttgccat cttcgaccac tttttgcagc gctgtacctg
    17161 cacccagcca ggcggggagc atcagacggt tttgcgtcca ggcaaaaatc cacggaatgg
    17221 cgcgcagcga ctcgacgccg ccggtcgggc gacgtttcgc cggacgtgaa cccaacggca
    17281 gtttgcccag ttcttgttcc ggtgtagcgg agcggaagta aggcacaaaa tctttgtttt
    17341 cacgtacgta gccacggtag agatcgcagg agatgactga tagttcatcc ataatgcgac
    17401 gccagctctc tttcggctca ggtggtggca gcaggttggc ttccagaatc gccccggtgt
    17461 aaagcgacag gctgctgacg gtgatttctg gcagaccata tttaaagcgg atcatctcgc
    17521 cctgttcagt cacgcgcagg ccacctttca ggcttcctgg cggttgtgac agcagcgccg
    17581 catgagcagg tgcgccgccg cgaccaatgg aaccgccgcg accgtggaac aacgtcagct
    17641 caatacccgc tttttcgcag gttttgatta atgcatcctg tgcctgatat tgcgcccagg
    17701 aagctgccat cacgcccgca tcttttgctg agtcggaata gccaatcatc accatctgtt
    17761 tgccctgaat caggccgcga taccagtcga tattcagcag ctgggtcatg acatcgttgg
    17821 cgttgttcag gtcatcgagg gtttcaaaca gcggagcaac cggcattgca aacccgatac
    17881 ccgcttcttt cagcagcagg tggacagcca gcacgtcgga cggcgttttc gccatcgaga
    17941 tcacgtaggc ggcaatggag ccttgcggcg cttcggcaat cacctggcag gtatcgagca
    18001 cttcgcgcgt ttcggcgctt ggttgccagt tgcgcggcag aagcggacgt ttggagttca
    18061 gttcgcggat caggaacgcc tgtttgtcgg cctctgacca gctttcgtag tcgccgatac
    18121 cgaggtagcg ggtcagctcg cccagcgctt cggtatggcg ggtgctctcc tgacggatat
    18181 caatacggac cagcggtacg ccgaaacatt tcacgcggcg cagggtgtcg agcagatcgc
    18241 cgttggcgat aatacccatg ccacacgcct gaagtgactg gtagcaagcg tagagcggtt
    18301 cccacagttc ttcgttttgt gtcagcaggc cttctggttt tggcagttct tcgcctttca
    18361 ggcgcgcttc cagccatgcc tgtgtcgcca tcaggcgaga acgcaggttt ttcatcagat
    18421 agcgatacgg ttctgcggca ccttcttcgc caaccagcgc cagcagttca ggggtcgctt
    18481 caaccatcga cagttcagaa accagcacct gaatatcttt caggaacaaa tcggtggctt
    18541 tccaacggct gagcagcagg acgtggcggg tgatatcggc agtgacgttt gggttgccgt
    18601 cgcggtcacc gcccatccac gaggtaaaac gtaccggaac aaattcgacg ggcagtttgt
    18661 agccgaggtt ctcttccagt tgttcgttca gttcgcgcag gtaattcggt acgccttgcc
    18721 acaggctgtt ttctactacg gcaaagcccc atttggcttc atctaccggg cttggacgca
    18781 gcttacggat ttcatcggta tgccatgact gggcgatcaa ctggcgcagg cggcgcatca
    18841 gctggttgtg ttcgtagtca gcgatatctt tgttatcgag ctgttttaaa caggcgttca
    18901 cttccaccat tttgtggatc agtgtacgac gggtaatttc ggttgggtga gccgtgagga
    18961 ccagttccag cgacagcgat tccactgctt ttttgatggt gtcttcgctc agttccggct
    19021 ggtttttcag tttacgcagg gtgcgggcga tcacttccgg gttgctggca gcttcgcctt
    19081 tcggcgaaat gctgtggtat tgctcggcgg tgttggccag gttcaggaac tgactaaacg
    19141 cacgcgcaac gggcagcagc tcgtcgttcg acaaattttg taaggtggtg agcaactcct
    19201 ggcggttagc atcattgcca gcgcgtgaag atttggacaa cttacggata gtttctacgc
    19261 gttcaagaat gtgttctccc aacgcatcct tgatggtttc tcccagcact ttgccgagca
    19321 tactgacatt actacgcaat gcggaatatt gttcgttcat attaccccag acaccccatc
    19381 ttatcgtttg atagccctgt atccttcacg tcgcattggc gcgaatatgc tcgggctttg
    19441 cttttcgtcg tcttttataa agccacgtaa aagcggtgac gtcaaatgct gcgaaatcgc
    19501 ttcagcaaac gaataaatag caggaattta cgtcattaaa ttcacgacgc tttaaataag
    19561 cgtaacttat gggaatgtta aaaaatcgcc tcaagtaaca ccaaaggtgt aggtcggata
    19621 agatgcgcaa gtatcgcatc cgacattatt gcggcactgg agtttggcaa cagtgccgga
    19681 tgcggcgcga gcgccttatc cggcctacgg ttgggcatcg tttga
//
LOCUS       DAZKFU020000071        19628 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZKFU020000071 DAZKFU020000000
VERSION     DAZKFU020000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.071"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            216..716
                     /gene="tssB"
                     /locus_tag="ACHOQQ_RS10080"
                     /old_locus_tag="pgaptmp_002016"
     CDS             216..716
                     /gene="tssB"
                     /locus_tag="ACHOQQ_RS10080"
                     /old_locus_tag="pgaptmp_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4470273.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            751..975
                     /locus_tag="ACHOQQ_RS10085"
                     /old_locus_tag="pgaptmp_002017"
     CDS             751..975
                     /locus_tag="ACHOQQ_RS10085"
                     /old_locus_tag="pgaptmp_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470274.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1026..2501
                     /gene="tssC"
                     /locus_tag="ACHOQQ_RS10090"
                     /old_locus_tag="pgaptmp_002018"
     CDS             1026..2501
                     /gene="tssC"
                     /locus_tag="ACHOQQ_RS10090"
                     /old_locus_tag="pgaptmp_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU4470275.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2508..2921
                     /gene="tssE"
                     /locus_tag="ACHOQQ_RS10095"
                     /old_locus_tag="pgaptmp_002019"
     CDS             2508..2921
                     /gene="tssE"
                     /locus_tag="ACHOQQ_RS10095"
                     /old_locus_tag="pgaptmp_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGU4470276.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2925..4775
                     /gene="tssF"
                     /locus_tag="ACHOQQ_RS10100"
                     /old_locus_tag="pgaptmp_002020"
     CDS             2925..4775
                     /gene="tssF"
                     /locus_tag="ACHOQQ_RS10100"
                     /old_locus_tag="pgaptmp_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4470277.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTMGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4739..5821
                     /gene="tssG"
                     /locus_tag="ACHOQQ_RS10105"
                     /old_locus_tag="pgaptmp_002021"
     CDS             4739..5821
                     /gene="tssG"
                     /locus_tag="ACHOQQ_RS10105"
                     /old_locus_tag="pgaptmp_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGU4470278.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALIRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5846..7126
                     /gene="tagH"
                     /locus_tag="ACHOQQ_RS10110"
                     /old_locus_tag="pgaptmp_002022"
     CDS             5846..7126
                     /gene="tagH"
                     /locus_tag="ACHOQQ_RS10110"
                     /old_locus_tag="pgaptmp_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HGU4470279.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7123..7647
                     /gene="tssJ"
                     /locus_tag="ACHOQQ_RS10115"
                     /old_locus_tag="pgaptmp_002023"
     CDS             7123..7647
                     /gene="tssJ"
                     /locus_tag="ACHOQQ_RS10115"
                     /old_locus_tag="pgaptmp_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU4470280.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYMTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7650..8981
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS10120"
                     /old_locus_tag="pgaptmp_002024"
     CDS             7650..8981
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS10120"
                     /old_locus_tag="pgaptmp_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4470281.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8986..9747
                     /gene="icmH"
                     /locus_tag="ACHOQQ_RS10125"
                     /old_locus_tag="pgaptmp_002025"
     CDS             8986..9747
                     /gene="icmH"
                     /locus_tag="ACHOQQ_RS10125"
                     /old_locus_tag="pgaptmp_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HGU4470282.1"
                     /translation="MDEGSLSLPPFTGYDEKGQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILYQLNKLLR"
     gene            9756..12536
                     /gene="tssH"
                     /locus_tag="ACHOQQ_RS10130"
                     /old_locus_tag="pgaptmp_002026"
                     /gene_synonym="clpV"
     CDS             9756..12536
                     /gene="tssH"
                     /locus_tag="ACHOQQ_RS10130"
                     /old_locus_tag="pgaptmp_002026"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGU4470283.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIDMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYILPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSVVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEVAWHQQQTLVREIIALRQQLLGMAEDT
                     DAVVDTSSESESESESESESESESEQDNTAAIPADDAGSVQPEETAETVSPVQRLAQL
                     TAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPVWLGDTIK
                     GQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYL
                     TTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVL
                     NLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPEIMQEALYPVLAD
                     FFKPALLARMEVVPYLPLSKETLATIIAGKLTRLDNVLRSRFGAEVIIEPEVTDEIMS
                     RVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAADGAFTADVEDAQ
                     DDQSVTEDEPVL"
     gene            12533..13276
                     /gene="vasI"
                     /locus_tag="ACHOQQ_RS10135"
                     /old_locus_tag="pgaptmp_002027"
     CDS             12533..13276
                     /gene="vasI"
                     /locus_tag="ACHOQQ_RS10135"
                     /old_locus_tag="pgaptmp_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGU4470284.1"
                     /translation="MSRMRVFRQTGWFIAGLMAGLPASAAPAETSSMAGVAVAVATAT
                     PLDAAATLQAMQSCRRESAALERLDCYDRLLEPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13281..14696
                     /gene="tssA"
                     /locus_tag="ACHOQQ_RS10140"
                     /old_locus_tag="pgaptmp_002028"
     CDS             13281..14696
                     /gene="tssA"
                     /locus_tag="ACHOQQ_RS10140"
                     /old_locus_tag="pgaptmp_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU4470285.1"
                     /translation="MAMDLRDPNVWISHLLENLPDEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSPQTTATSESGVTQPSTPTPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMTLWQQVEKSVLLAPYWLDGHCISAQTAHRLGYTAVADAIR
                     DEVVSFLGRLPALADLLFNDHTPFVSEKTKQWLATQPGNQAAPVIQVSNEDIQAARLC
                     FDEQGLEAALRYLDTLPAGEPRDQFHRQYFGAQLMEEAGLVQLAQQQYRMLFRMGLQM
                     MVADWEPSLLEQLEQKFTAEQ"
     gene            14805..18239
                     /gene="tssM"
                     /locus_tag="ACHOQQ_RS10145"
                     /old_locus_tag="pgaptmp_002029"
     CDS             14805..18239
                     /gene="tssM"
                     /locus_tag="ACHOQQ_RS10145"
                     /old_locus_tag="pgaptmp_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGU4470286.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVQVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPSVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18250..19628
                     /locus_tag="ACHOQQ_RS10150"
                     /old_locus_tag="pgaptmp_002030"
                     /pseudo
     CDS             18250..19628
                     /locus_tag="ACHOQQ_RS10150"
                     /old_locus_tag="pgaptmp_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
BASE COUNT         4559 a         5420 c         5466 g         4183 t
ORIGIN      
        1 cacaggttca tacaatctat ttaacgcttg cagaataccc ctctttacga tcgataaaaa
       61 gtccttttct tatttatttt gaagcgtaaa atgcggggtg atgaaggagt ggtgatttta
      121 ttttcaccgc catcaaggcg gcatttccga tattttttgt ggtacgaata tgccgtgttc
      181 atggcctgta cgagataaca tgacggatca cttttatgag caaaaaattt gaaggcagcg
      241 tcgcgcctcg cgaacgtatt aatatatcgt atgtcccaaa aacagatggc cagacggctg
      301 aagtggaact tccgcttaac atgctcgttg tcggcgatac cggaaatacg caggaaacat
      361 cctcactgga tgaacgtcag gcagtttccg ttaacaagca caatttcggt gcagtgatgg
      421 ctgaagcagc cattggcctc aattttaccg tccccgccac gctgaaggga agcacaacgg
      481 acgatgagct gaatgtggcg ctgaatatca agtcactgga tgatttttca ccagatagcg
      541 tggcccggca ggttccggaa gtgaataaat tgctggaact gcgtgaagca ctgactgcac
      601 tgaaggggcc gatgggtaac cttccggcat tccgtaccca gctccaggca ttgctggaaa
      661 atgaagagtc ccgcgagcag ttgctcaaag aaatcggcca ggtgagcaat aaataaccac
      721 cactatttca ttaaacaggg ttatccaggc atgacggcac acaaaaatat ctccgggaca
      781 taccacctct ctgttgcaga cattctgcag gtggtttacc aggtatgttt cagcccgtcc
      841 gttgaaatca accaggatgg tgtggctgcg ctgattacaa cactggatcg ccgtatcagt
      901 gatctattgg atgagattat ccatttttgt gagtttcagc agagcgcgtc gcactggcag
      961 cgcgtacttc actgacggcg aaaagccggt ttcagaaaca cagctaaaac agaaggattt
     1021 tcagtatgtc tttacaggaa gaggaactcg tttccagtca tgccgggcag cctgagcagg
     1081 catcgtcgct gcttgatcaa atcatggcac agacccgtat tcagcccggt tcggaaggtt
     1141 atgacgttgc ccgccagggt gtgacagcct ttattgccag catcctgcaa tccactgcat
     1201 ctgccgaacc ggtcaataaa ctggccgtgg acagcatgat tgcagatatt gatgaacgca
     1261 tcagccgcca gatggatgtc atcatccatg cgcctgcctt tcagcaggtc gagtcgttct
     1321 ggcgctcact gaaaaccatg gtggatcgcg ttgatttccg tgaaaacatc aaggtcaacg
     1381 tgcttcatgt caccaagcag gaacttctgg aagactttga gtttgcgcca gaaattattc
     1441 agtccggatt ttataagcac gtttattcgt ccggtttcgg acagtttggc ggtgaaccca
     1501 tcgctgccgt gctgggcgct tatgagttca aaaataccgc gcctgacatg aaactgctgc
     1561 aatacgtcag tgccgtgggc gcaatggcgc acgcaccgtt cctgtcctcc gtttccccgg
     1621 agtttatggg gctgaactcg tggaccgaac tgcctaatat caaagatctt tatgccatct
     1681 ttgaaggtcc ggcctacacc aaatggcgcg ccctgcgtga ctcggaagat tcgcgctatt
     1741 tagggcttac ggcaccccgc ttcctgcttc gccagcccta ttcaccaaca gataaccctg
     1801 ttaagaactt taactattac gaagatgtca gccagaacca cgaagattat ctgtggggta
     1861 atacggcctg gatgctggca tgcaatatcg cagacagttt tgccaaatac cgctggtgtc
     1921 caaacattat cggtccgcaa agcggcggcg cagtgaaaga tctgccggtg catctgttcg
     1981 aaacgatggg gcagattcag gccaaaatcc caaccgaggt gctggtcacc gatcgccgcg
     2041 aatttgaact ggctgaagag ggttttatca cccttaccat gcgtaaagac tctgataatg
     2101 cagccttttt ctctgcaaac tcggtacaaa aaccgaagca ctttccggga aaagatgcag
     2161 aaaccaacta taagctgggt acgcagcttc cttatctctt catcatcaat cggttagcgc
     2221 actacatcaa ggtgttgcag cgtgaacaac tggggtcatg gaaagaaagg agcgacttag
     2281 aaagagagct taatacctgg atccgtcagt atgttgccga ccaggaaaac ccgcctgcag
     2341 acgtgcgcag ccgcaaacct ctgcgcgctg ccaaagttga ggttatggat gtagaaggcg
     2401 aacccggctg gtaccaggtt gcgctaagcg tgagacctca ttttaaattt atgggggcaa
     2461 attttgagct ttccctggtt ggccggttag acagggagta acacaccatg ataccggatc
     2521 gggaaagaac cacaggttct ctttttgagc gtatggaagc ctcttcggcc agaaaccggc
     2581 agggaggcag tattcattcg ctcaggcaat ccattcgcca gaacctacga aacattctta
     2641 atacccggtc cggtagttgc cggggtgcgc ctgagctggg cattgatgaa ccggaagggg
     2701 ctgaaaactt ccgcgaatcc atgagtcggg ccatcgagca atgtatagaa cgctatgaac
     2761 cccggatctc acacgctgaa gtgcaggcag tggtttcatc cgcctccagc ccgctggata
     2821 tgacgtttca tattacagcc tgggtgacat ttaacgaaac gcatgaggtg cttgaatttg
     2881 atatggcacc gaatggcagc cagcattacc gggtggactg acaaatggaa tttgaagaac
     2941 gctattttcg ggaagaactc gattacctgc gccagcttag caagctgctg gcaacggaaa
     3001 aaccccatct ggcccgcttc ctggccgaaa aagatgcgga tccggatatt gaacgcctgc
     3061 tggaaggggt ggcttttctt accggcaatc tccgccagaa aattgaggat gaattcccag
     3121 aactgacgca cgggcttatt aagatgctat ggcctaatta cctgcgtccg gttccggcaa
     3181 tgacccttat tgaatatacg ccggatatgg ataagtcttc tgtaccggtg ttaatccccc
     3241 gtaatgagca gtttacaacc aacgccgggg aaatcagagt tgatgaagtg ctgccctctg
     3301 atgctaaaaa ggaggagccg cctccctgta ccttcaccct ctgccgggat atctggctgc
     3361 tgcccgttcg cctggagcag attgaaaacc gcagtacgac ccgtaatggt gttatcaaca
     3421 tcaccttttc ggtcgcaccg ggaacggact tccgcacgct ggatctgaac aaacttcgct
     3481 tctggctcgg caatgacgac aactataccc gtgaccagct ttatttatgg ttctgcgaat
     3541 acttgcaggg tgccgacctg actatgggtg aacagcatat tcgcctgcct gagtttatgc
     3601 taaaagctgt cggttttgag ccgcaggatg ccatgctgcc ctggccgaaa aacgtccaca
     3661 gcggctaccg gatccttcag gagtatttct gttaccccga tgcgtttctc ttttttgatc
     3721 tttgtggttg tccggctttg cctgacggat tgcaggcgga attctttacc ctgcaactgc
     3781 gtttttcgcg ccctttgccc gtggacatcc ggctgcgccg cgattccctg cgcctgtatt
     3841 gcgcacctgc cattaattta tttatccacc atgcagaagc catcacgctg gacaaccggc
     3901 gggcagacta tccgctggtt cccagccgcc attacccaca acattacgat gtattttccg
     3961 ttaacagtgt ggtgagccag gtccaggata tgttcaggaa aaaagatctg gggcgtcctg
     4021 tttcgacgca ggccgcgcgc cagtggccag cctttgaaag tttcagccat cagatggaat
     4081 acagccggaa gcgggaagtg gtgtactggc atcaccggac caaaacatcc ctgttccatc
     4141 gcggctttga tcataccctt gcctttatac atgctgatgg cagttatccg tcagacgaat
     4201 ctctgctcag taatgaagtg gtttcggtat cgctgacctg taccaaccgt gagcttccgt
     4261 cacaaattcg ttccggcgat atcaccggca caaccggtaa aaatgcggct gttgcttcct
     4321 ttcgcaacat tacccgcccg acgcaaccac tctggccggt cattgatggc agcctgcact
     4381 ggtccctgct ctccgccatg aacctgaatt atctgtcatt actggatacg gacgcgctga
     4441 agcaggtcat cgctaacttt gatcgccacg caatccacca tccgcagacg gcgcggctgt
     4501 cacaacaaaa gctggatgcc attgagcgtc tggagacccg ccccgttgat cgcctgttta
     4561 cgggtattcc cgtccggggg ctggcctcca cgctgtatct gcacccggag ccgtttgtct
     4621 gtgaagggga aatgtatctg ctcggtacgg tgctttcgca ttttctgtcg ctgtacgcca
     4681 gcgttaactc attccacatg ctgaccgttg tgaacacaga aagccaggag acatggaaat
     4741 ggacggaaag aatcgggcag catcctctta tctgagtccg ggaaatcccc cggcagataa
     4801 agagcagaat gatccgctgg cacaggtttt ccataatgcc tgctcttaca atttttttgc
     4861 gatggcggag ctgttgcacc gcctggcaaa gggtgaaaag gggacgccag aattatccct
     4921 gcgcgacgat ccggcacagg aaaccctgcg tttcagcgcc gatgccagcc ttgccttccc
     4981 gtgcagtgat atcagtgcgc tgatacggga tacgtcaggg gcattcagga tgaccaccac
     5041 ctttatgggg ttacagggga gccagtcgcc tctccccggt tattacctcg atcacctggc
     5101 ctggaaagcg gtacatgaac aaagtccggt aggtgatttt ctggatatgt tcagccaccg
     5161 cctgacccag tttgtctggc atatctggcg taagtaccgc tatcacatca gttttcgtaa
     5221 cggcggtgtg gatgcgttct cgcagcgcat gtactccctt gtcgggctgg gccaccgcca
     5281 gcttcgcgat aagctggcga ttaatcacag caaaatgctg gcgtattccg ggatcctggc
     5341 aaatccggga cgatccccgg aaattatctg tggcctggtg tcccactgct ttgatttgtc
     5401 agaagtgacg ctgcaaaact ggcaacgtcg caaggtggat attgaaccgg atcagcaaaa
     5461 tagtctgggg agctattctc ttaaaaatgg cgaaaaactg gcgggaagat cggtactggg
     5521 caactttgtt ctgggtacac gagtgcccga tctcagcgga aaattccagc tcagtattac
     5581 cagcctgacc cgaaaacagt tcctctcttt tctgccgtcc ggcgaaaact tcctgccact
     5641 gacgatgttc gtgtccttca tcctgcgcga tcagcttgcc tgggacttac atctggggct
     5701 ggcgccggag caggtgggcg caatgcgcct gggcgataac aaaagtgcgc tgctgggctg
     5761 gaccagcttc ctcggcacac cggaagaacg accctcagtc accatcaggg tacggtcgta
     5821 aattatgata atcacggagc agaatatgcc ggaagagaaa ctacagacgc tttcattgca
     5881 ggtcattaac ggcagtgagc tggaaagcgg gcgggcggcg cgctgtctgt tcacacagca
     5941 gggaaatgtc ggccatggcc ccgaatgcca ctggtcggta caggatcgtc agcagagcat
     6001 tccggcccag gcttttaccg ttatcctgca cgatggcaca ttttgtctac gcccgcagac
     6061 ggcacaactg tggctgaatc aggcaaaagt cacagcaaca tcagacctga tacagttgcg
     6121 ccagggcgat gagatccaga tcggacggct gatggtgagg gttcatctga accggggaga
     6181 tattccccat tacgatgagg aaatggccac tcccgaaacc atcgttacca atcgcgatat
     6241 gctcacggat accctgctat caacggaggg tgcgccacac tatccgggaa tgactcaccg
     6301 gcaccagctt gcagacaccg tggtaaacgg tttttctgcc gatccactcc aggcacttca
     6361 gtccgaaagc ctgattacca cgggcgatcc gctttcaggc attgcggctg tccggccatc
     6421 ggcaccgctg tccgatccgg caagtaatgg ggggatcaat actccgttta tggatctgcc
     6481 gcccatttat gccagccctg gcgatcataa tgatgacatc tctgcggcag aaatggcgca
     6541 acgccacctt gcggtcaccc ccttactgcg cggtcttggc ggctcgctta ccgtgagcaa
     6601 ttccgacgat gcggatgatt ttctggagga ggccggacga acgttacagg ccgcaataaa
     6661 aggtctgctc gatttgcagc agcagcgtaa cagcctctca gacaaacatt tgcgcccgct
     6721 ggaagataac ccgctgcgcc tgaacatgga ttacgccacc gcgctcgacg tgatgtttgc
     6781 cgaaggtaaa agcccggtac atctggcggc tcccgccgcc gtcagtgaaa gcctgcgcaa
     6841 tgtccgccac cacgaagaag ctaaccgggc ggcgattgtg gagtcgcttc gtgtcctgct
     6901 ggacgccttc tcaccacaaa atctgctgcg ccgctttgtg cagtaccgcc gcagccatga
     6961 actgcgccag ccgctggatg atgccggagc atggcaaatg tacagccatt attacgaaga
     7021 actggcctcc gatcgccagc aggggtttga gatgctgttt aacgaggtct acgcccaggt
     7081 ctatgaccgg gtgcttcgtg aaaaacagcg ggagccggaa gcatgaacag gaaggcgttt
     7141 ctggcctgcg tgctgatgtg cgtcatatta accggctgcg aaacggcgaa aaaaatcagc
     7201 gaggtgatca aaaacccgga tatccaggtc gggagcctga aggaacaacc ttcggaaata
     7261 accgtcaccc tgctgactga accggacacc aataccaatg cggagggtga atcagccgcc
     7321 gtggatgtgc aactggtgta catgactgac gactcaaaat tgcaggcagc cgactatgac
     7381 cagatagcca gtacgccgct accggatgtg ctgggtaaaa actatatcga ccaccaggat
     7441 ttcaacctgt tgccggacac catcaaaacc ctgccgccgg taaagctgga tgagaaaacg
     7501 cagtttatcg gtgtcgtggc ctatttctcc gatgaccagg ccactgaatg gaaacaaatc
     7561 gagacggtgg aaggaaccgg ccaccattac cgcctgctgg tgcatgtacg tcaaagcagc
     7621 atcgaaatga aaaaagagga tgaataatta tggcaaccac ccgcaacaag gtgatgtggc
     7681 aggaagggat gctgatgcgc ccacaccatt tccagcaaca gcagcgttac aacgactacc
     7741 tggataacca gcgtttccgg gccatgaatg atttatcctg gggatttacc gaactcaccc
     7801 tcaacaatga actgctggcg cagggtaaga tcatgattga cagcgcgtca ggcacactgc
     7861 ctgacggcac cgtcttttct atccccgacc aggacgcact gcccgatccg ctgcacccgc
     7921 aaaattttcc ggacgaaaga agccgcaata tttacctcgc tctgccggtc gccagtgatg
     7981 tgagaaatga aatcagcgac gggcggcgaa tcgggcgtta ccggctgaat tatgccgatg
     8041 tccgggatct gcattcagaa gaaggcgacg cgcgaacact gacgctggga caactgacgc
     8101 cgcgcattat gagcggtgca gaagatatga gtgcctatat tacgctcccg ctttgccgta
     8161 tcagcgatcg ccatgccgac ggctccctga cgctggatga cgattttatc ccctcctgcc
     8221 agaatattca ggtcagtaag aaactgcgtg tttatctcaa agaggtacag ggggccattg
     8281 gcggacgggc aagcgatctg gcaaaccgca ttggctctcc ggcgcagagc ggcatcgcgg
     8341 atgtggcgga atttatgatg ttgcagttgc ttaaccgtaa ccagacccgg tttacccatc
     8401 gcgcccgccg atcccagctc cacccggaag atttctacct tgatcttgcc gggttgctgg
     8461 gtgaactgat gacctttacc gagccgtcgc gcctgccctg cccgcttgat gtgtatgatc
     8521 atcatgacct gaccaaaaca tttaaaacac tgttaccgga agtcaaacgg gcgctgcata
     8581 ccgtactgtc gccaagagcg gtcaatctgc cgctgcatct gcgtgacggc atctggcagg
     8641 ccgatgttca tgactcggaa ctgctacagt ctgccacctt tgtactggcc gtggcggcaa
     8701 atatgcctgt cgatcagatc cagcgtcagt ttatccagca gtcgaaaatt tcctcgccgg
     8761 aaaaaatccg caatatggtc agtgtgcaga tccccggcat tccattgcgt gccctgatgg
     8821 tggctccccg tcagcttcct taccattccg ggttcagcta tttcgaactc gacaagagcg
     8881 gacaggcctg gacagaaatg gctgccgccg gggccgttgc actgcatgta tccggcagtt
     8941 tcccggatct gaacatgcaa ctgtgggcga taagagggta agacgatgga tgaggggtca
     9001 ctgtctctgc caccatttac cggatacgac gaaaaaggcc agcgcgacta ccatctcgcg
     9061 cttcgtggca acagcctgaa tccgatgatc gacgcagcca caccgctact cggtatggta
     9121 atgcgccttt ccaccatgaa cagccagacg atgccggagc atctttttgc tcaggtggtg
     9181 acggatgttc aggccgtgga gcaactgctt caggaacagg gctacgaacc gggcgtgatt
     9241 atctcgtttc gctacatcct ctgcacgttt attgatgaag cggcgctcgg taacggctgg
     9301 tcgaacaaga acgagtggat caagcagtca ttgctggtcc atttccacaa cgaagcctgg
     9361 ggtggcgaga aagttttcat cctgctggag cgcctgatcc gtgaacccaa acgttaccag
     9421 gatctgctgg aatttctctg gctctgtttt tctctgggat ttcgcgggcg ctacaaagtg
     9481 gcagcacagg atcaggggga atttgagcag atctaccgcc gtctgtacca cgttctgcat
     9541 aaactgcgcg gcgatgcccc tttcccgctg ttacaccagg acaaaaaaac ccagggcggg
     9601 cgttatcagc tcatcagtcg cctgaccatt aaacatattt tttgtggtgg cgttgtcgtg
     9661 ctggcgttgt tttacctgtt ctatctgttg cggctggaca gccagacgca ggacatcctg
     9721 taccagctaa acaaattact tcgataagga cgtttatgat ccagattgat cttcccacgc
     9781 tggtaaaacg gctgaacctg ttctcccgcc aggcgctgga gatggccgcc tcagaatgta
     9841 tgagccagca ggcagcggaa attaccgtca gccatgtgct tattcagatg ctcgccatgc
     9901 cacgcagtga cctgcgggtt atcactcggc agggcgatat tgacatggaa gagttgcgcc
     9961 aggcgctgac ggtggagaac tacacaaccg cccgttctgc ggacagctac ccggcgtttt
    10021 ccccgatgct ggttgagtgg ctgaaagagg gctggctgct ggcgtcggct gagatgcagc
    10081 acagcgaact gcgcggcggc gtgttgctgc tggccctgct gcattcgccg ctgcgttata
    10141 tactgcctgc tgccgcccgg ctgttgaccg gtattaaccg tgaccgtctg caacaggact
    10201 ttgtgcagtg gacacaggag tcggcggaat cagtcgtgcc ggatgcagac ggtaaaggcg
    10261 caggcacact gacggacgcc tctgacaccc tgcttgcccg ctacgccaaa aacatgaccg
    10321 aagatgcccg taacggcagg cttgacccgg tactgtgccg cgaccacgaa atcgacctga
    10381 tgatcgacat tctctgccgc cgccgtaaaa acaacccggt ggtggtgggc gaagcgggcg
    10441 tgggcaaaag cgcgctgatt gaaggtctgg cgctgcgcat cgtggcaggt caggtgccgg
    10501 acaagctgaa aaacaccgat atcatgaccc ttgacctggg cgcattgcag gccggggcat
    10561 cggtgaaggg cgaattcgaa aaacgtttca aggggctgat ggcggaggtc atttcctctc
    10621 cggtgccggt cattctgttt atcgacgaag cgcataccct gattggcgcg ggcaaccagc
    10681 agggcgggct ggatatctcc aacctgctca aaccggcgct ggcgcgcggc gagctgaaaa
    10741 ccatcgccgc caccacctgg agcgagtaca aaaaatactt cgagaaagac gccgccctgt
    10801 cgcgccgctt ccagttggtg aaggtcagcg aacccaacgc cgccgaagcc accattattc
    10861 tgcgtggtct gtcggtagtg tatgaacagt ctcacggcgt gctgattgat gatgacgcct
    10921 tgcaggccgc agcaacgctg agcgagcgtt atctctccgg gcgtcagttg ccggacaaag
    10981 cgattgatgt gctggatacc gcctgcgccc gtgtggccat caacctgtca tcgccaccaa
    11041 agcaaatctc ggcgctgacc accctgagcc accagcagga ggcggaaatt cgccagcttg
    11101 agcgcgagct tcgcatcggg ctgcgtaccg acacctcacg gatgaccgag gtgctggtgc
    11161 agtatgatga aacgctgacg gcgctggatg aactggaagt cgcctggcat cagcagcaga
    11221 cgctggtacg ggaaattatc gcgcttcgcc agcagttact gggtatggca gaggacacag
    11281 atgccgtagt ggatacgtcg tcagagtcag agtcagagtc agagtcagag tcagagtcag
    11341 agtcagagtc agaacaggat aataccgctg ccataccggc tgatgatgct ggcagcgtac
    11401 agccggaaga aaccgctgaa acagtttccc cggtacagcg actggcacag cttactgccg
    11461 aactggacgc cctgcataac gaccggttgc tggtttcccc gcacgtcgat aaaaaacaga
    11521 ttgcggcggt gattgccgaa tggaccggcg taccgcttaa ccgcctgtcg cagaatgaaa
    11581 tgtcggtcat caccgacctg ccagtatggc tgggcgacac catcaaaggc caggatctgg
    11641 cgattgccag cctgcacaaa cacctgctga ctgcacgcgc cgacctgcgt cgtccgggac
    11701 gtccgcttgg cgcgttcctg ctggctggcc ccagcggtgt gggtaaaacc gaaaccgtcc
    11761 tgcaactggc ggagctgctc tatggcggtc gccagtacct gaccaccatc aatatgtccg
    11821 agtttcagga gaaacacact gtctcgcggc tgattggttc ccctcctggc tatgtcggct
    11881 atggcgaagg cggcgtactg accgaagcca tccgccagaa accgtactcg gtggtactgc
    11941 tcgatgaagt ggaaaaagcg caccctgatg tgctcaacct gttctaccag gcgttcgaca
    12001 agggcgagat ggcagacggc gaaggccgcc tgattgactg taagaatatc gtcttcttcc
    12061 tcacgtccaa cctcggctac caggtgattg tcgaacacgc ggatgacccc gagatcatgc
    12121 aggaagccct ctatccggtg ctggcggact tctttaaacc tgccctgctg gcgcgaatgg
    12181 aagtggtgcc gtacctgccg ctgtcgaaag agacgctcgc caccatcatt gccgggaaac
    12241 tcacccgtct ggataacgtg ctgcgcagcc gctttggcgc agaagtgatt atagagccgg
    12301 aagtgacgga cgaaatcatg agccgtgtca cccgcgcgga aaacggcgca aggatgctcg
    12361 aatcggtcat cgatggcgac atgctgccgc cgctctcgct gctgctgttg cagaaaatgg
    12421 cggccaatac ggcgattgcc cgtatccgcc tgtcggcggc agacggcgcg ttcacggcag
    12481 atgtggaaga tgctcaggac gaccaatccg tcacagagga tgaaccggtt ttatgagcag
    12541 gatgcgcgtt ttccggcaaa caggatggtt tatcgcgggc ctgatggcgg gcttacccgc
    12601 cagtgccgcg cccgcagaaa catcgtcaat ggccggtgtg gcggtagccg ttgccacagc
    12661 gaccccgctg gatgcggcag ccacgttaca ggccatgcag tcctgtcggc gggagtctgc
    12721 ggcgctggag cggcttgact gctatgaccg cctgctggaa ccgctgtccc cgtcagggtt
    12781 tgacggggcg ctggtcaaag ctggttttgt gggcgaagcc tggacccgcg ccacagagca
    12841 ggaaaaacgc cgccagggca acaccacaga gctgctggtg acgcaggtgc cgggggaacg
    12901 cccgacggtg gtgataacca ccccggctat cggccatgtt ccgccacgcc cggtgctgat
    12961 gttcagttgc gtggacaata ttacccggat gcaggtggcg ctgatgcacc ctctggatgt
    13021 ccatgatatc gccgtcaccc tgaatgccga cagccgggca ttacgcagcc actggtttgt
    13081 gcgcgagaac ggcaccctgc tggagtccag ccgggggtta tccggcattg atgaaattaa
    13141 gcaactgttc ggcgcaaaaa cgctgacggt ggatactggc gcagacaacg ccgccggaaa
    13201 gctgaccttt aacattgacg ggctagcaag ggcgattgct cccctgcgcg atgcctgcca
    13261 ctgggcggga gaataaaaat atggcaatgg acttacgcga tccgaatgtc tggatatcgc
    13321 acctgctgga aaacctgccg gatgagaaac tggcatcggc gttgaaagac gacaacccgg
    13381 actgggagta tatcgacggc gaaatcgtca aactggggtc actggcccac agccagctgg
    13441 atatcccgga actccagcgc cgggggctgg tcattctggc ttcggagagt aaggattttc
    13501 gtctgctggc gcatctgctg agaaccctgc aacacgccgg tgatcctcat ctcgcattgc
    13561 gcctgctggc gctgtatgtg gagcattact ggtcggtggc tgcgccgcag aatatggcac
    13621 ataaaaaacg ctttgccagc caggtcatta aacgctttga aacaggtatt gaaggctttt
    13681 cacagaacgc cgccacagcg cagcgcgacg cgctgtcagg ggaactggcg aaactggcgc
    13741 agtgctggca gtcacataac gccccggaac tggcactggc taccgatgat ctttttgccc
    13801 tgtaccagcg tgcgtttaat cgtgcggcac cggctcctgc ccccactccg gcggcctccg
    13861 atagttcacc acagaccacc gccacgtctg aaagcggcgt gacgcaaccc agtactccga
    13921 ctccccaaat cgccatcgac agtcacgacg acaaagcctg gcgcgacacg ctgttaaaag
    13981 tggcggctat tttatgtgaa cgccagccgg actcgccgca gggctatcgc ctgcgccgcc
    14041 atgccctgtg gcaatccatc accagtacac cccaggcgga aagcgatgga cgtaccccat
    14101 tggctgcggt ctctgccgat atggtggcgg attaccagtc ccggcttgcc agcgcggata
    14161 tgacgctgtg gcagcaggtg gaaaaaagcg tactgctggc accctactgg ctggacggtc
    14221 actgcatttc tgcacagacg gcgcaccgtc ttggctatac cgctgtggcc gatgccatcc
    14281 gcgatgaagt cgtttccttt cttggccgtc tgcccgcgct cgctgatctt ctgtttaacg
    14341 accacacacc gtttgtttct gaaaaaacga agcaatggct tgcaactcag ccaggtaacc
    14401 aggccgcgcc agtcatacag gtgagtaatg aagatattca ggctgcgcgg ttgtgttttg
    14461 atgaacaggg gctggaagcc gccttacggt atctggatac gttgcccgcc ggtgaacccc
    14521 gcgaccagtt tcaccggcag tattttggcg cgcagcttat ggaagaggcg gggctggtac
    14581 agcttgcgca gcagcagtac cggatgttgt tcaggatggg attgcagatg atggtggcag
    14641 actgggagcc gtccctgctt gagcagctag aacaaaaatt cacggcagaa caataagcga
    14701 taacaggagt ttttgtgttc agattaccaa caccccgact actcagcgga ctcaaatcag
    14761 ccctacgccc ggcgatgccc cggtttaaag tctccgcttt ctggctgctg atactggcgt
    14821 ggatttttct gctggtatgg atctggtgga aaggcccgac gtggacgctg tatgaagaac
    14881 agtggctcaa accactggcg aaccgctggc tggcaacggc ggcatggggg attatcgccc
    14941 tggtgtggct taccgtccag gtgatgaagc gcctgcaaca acttgaaaaa atgcaaaagc
    15001 agcagcgcga ggaggccatt gatccgctca gtgtggagct gaacgcccag cagcgttacc
    15061 ttgaccgctg gctgctgcgc ctgcaacgcc atctcgacaa ccgccgtttc ctgtggcagt
    15121 tgccgtggta tatggtcatc ggcccggcgg gcagtggcaa aacgacactg ctgcgcgagg
    15181 ggtttccgtc agacattatt tatgccccgg agggcgcgcg cggcacagaa caacgcctgt
    15241 accttacgcc ccatgtcggt aaacaggcgg ttatctttga tatcgacggc acactctgcg
    15301 ctcccgctga tgcggatatc ctgcatcgcc gtctgtggga acatgccctc ggctggctga
    15361 aagaaaagcg cgcgcgccag ccgctgaacg ggattattct gacactcgat ttacccgatc
    15421 tgcttaccgc agacaaacgc cgccgcgagc atctgttaca gacgctgcgc agccgcttgc
    15481 aggatatacg ccagcatctt cactgccagt taccggttta cgtggtactc acccggcttg
    15541 atttattgca gggttttgcc gccctgttcc agtcactgaa cagacaggat cgcgatgcga
    15601 ttctgggcgt cacgttcacc cgccgcgccc atgaaaatga tgactggcga acagagttga
    15661 atgctttctg gcagacatgg gtggatcgaa tgaacctagc gttgccggat ctgatggtcg
    15721 ctcagactca cacccgcgcg tctttattta gtttttcccg ccagatgcag ggaagccgtg
    15781 aaccgctggt gtcactgctt gagggtctgc ttgatggcga aaatatgaac gtgatgctgc
    15841 gtggtgtcta tctcacctct tcgcttcagc gtggacagat ggatgatata ttcacccagt
    15901 ctgccgcccg ccagtaccgg ctgggcaata acccactggc ttcctggcct ctggtggaca
    15961 ccgcgcctta tttcacccgc agcttgttcc cgcaggcatt actcgcagag cctaatctgg
    16021 caacggagag ccgcgcctgg ctgatgcgtt cccgtcgccg cctgacggtt ttctctacca
    16081 caggaggcgt ggcggcactg ctgctcatca ccggctggca tcactattac aacggtaact
    16141 atcagtccgg catcaccgtg cttaagcagg ccaaagcctt tatggacgtg ccgcctccgc
    16201 agggggaaga tgactacggc aacctgcaac tgccgctgct gaacccggta cgtgatgcca
    16261 cactggccta tggcgactgg ggcgaccgca gccgtctggc cgatatggga ctgtaccagg
    16321 ggcgacgtat cgggccatat gtggaacaga cctatctgca actgctggag caacgttacc
    16381 taccctcgct gtttaacggg ctggttaaag cgatgaatgc cgcgccgccg gagagtgaag
    16441 aaaaactcgc ggtgctgcgc gtgatgcgaa tgctggagga caaaagcgga cgtaacaatg
    16501 aggtggtgaa gcagtatatg gcaaaacgct ggagcgaaaa gttccacggc cagcgcgata
    16561 tccaggcaca actgatgtcc catcttgact acgcgctggc tcatactgac tggcacgcag
    16621 agcgtcaggc gggcgacggt gacgccatca gccgctggac gccatatgac aagcccgtgg
    16681 tatcagcaca gaaagaactg agcaaactgc ctgtctacca gcgggtttac cagagcctga
    16741 aaacgcgggc gctgggcgtt cttcctgccg acctcaatct gcgtgaccag gtagggccaa
    16801 cctttgatca ggtatttaca tctgccgatg acaacaaact ggttgttcca cagtttctta
    16861 cccgttacgg cctgcaaagc tattttgtaa aacagcgcga tgaactggtt gaactgacgg
    16921 cgatggattc ctgggtactt aaccttaccc gcagcgtgaa atacagtgac gccgaccgcg
    16981 cggaaatcca gcgccagctc accgagcagt atatcagcga ctacaccgcc acctggcggg
    17041 ccgggatgga caatctgaat atccgcaatt ttgagtccat cggacaactg accggggcgc
    17101 tggagcaggt tatcagcggc gaccagcctt tgcagcgggc gctgaccgtg ctgcgtgaca
    17161 acacacagcc aagcgtcttt tctgaaaaac tctctgccaa agaacgggat gaggccctgg
    17221 cagagccgga ctaccagtta ctcacccgtc tcgggcatga gttcgccccg gaaaacagta
    17281 ccctggcagt acagaaagac aaagaaagca cgatgcaggc agtatatcag caactcaccg
    17341 agttgcaccg ctatctgctg gcgatccaga acgcgcctgt accagggaaa tcggcgctga
    17401 aagccgtgca gttacggctt gatcagaaca gcagcgatcc gatattcgcc acccgccaga
    17461 tggcaaaaac gctgcctgct ccgctcaacc gctgggttgg caggctggct gaccaggcct
    17521 ggcatgtggt gatggtggag gccgttcact atatggaagt ggactggcgc gacagcgtgg
    17581 tgaaaccgtt taacgagcaa ctggcaaata actatccgtt taatccgcgt tctgcacagg
    17641 atgcctcact ggatgccttc gaacgcttct ttaaaccgga tggcatactg gataccttct
    17701 accagcagaa cctgaagctg tttatcgata atgacctgag tctggaggat ggcgataaca
    17761 acgtcattat tcgcgaagat attattgcgc aactggaaac tgcgcagaaa atccgtgaca
    17821 tcttcttcag caaacagaac ggtctgggaa catcctttgc cgtggaaacg gtatcgcttt
    17881 caggcaataa acgccgcagt gtactgaacc ttgacggtca gttagtcgat tacagccagg
    17941 gccgtaacta taccgcccat ctggtctggc ctaacaacat gcgcgaaggc aacgaaagta
    18001 agctgacgct catcggcacc agcggcaacg cgccgcgcag tatcagcttc agcgggccgt
    18061 gggcgcagtt ccgcctgttc ggggccggac aactgaccgg agtacaggat ggcaacttta
    18121 ccgtgcgctt tagcgtggac ggtggcgcga tgacctaccg tgtgcatacc gacacggaag
    18181 ataacccgtt cagcggtggg ttgttcagcc agtttggtct gtcagacaca ctgtactgag
    18241 gaggcaggga tgaatagtaa cgtactgaca caaactatcg ttaccggcag tgacccgcgc
    18301 gggctgccgg aattcagcgc catccgcgag gaaataaaca aagccagcca cccgtcacag
    18361 cctgagctga actggaaact ggtggagtcg ctggcgctgg cgatttttaa agccaacggt
    18421 gtggatttac acaccgccac ctactatacg cttgcccgga cacggataca gggactggcg
    18481 ggattctgcg aaggtgcgga actgctggcg gcaatggtaa gccacgactg ggataagttc
    18541 tggccgcagg gcggcccggc gcgtactgaa atgctggact ggtttaactc ccgaaccggc
    18601 aatattctgc gtcagcaaat ctcctttgcg gaatctgatc tgccgctgat ctaccgcaca
    18661 gagcgggcat tgcagcttat ctgcgacaag cttcagcagg tagagctgaa gcgcgttccg
    18721 cgcgtggaga atctgctcta ttttatgcag aacacgcgta aacggcttga accccagctg
    18781 aagagtaaca ctgagaacgc cgcacagacc acggtcagaa cgctgattta tgccccggaa
    18841 acacagccat cttccacacc agaagcggta gtgcctcccc tgcccggcct gcctgagatg
    18901 aaagtggaag tgcgcagtct gacagagaat cccccacagg ccagtgtgat aaagcaaggc
    18961 agtacggtaa gagggtttat cgcagggatc gcctgttcag tggctgtcgc ctcagcattg
    19021 tggtggtggc aggtctatcc ggtgcagcag caactgttac aggttaacga caccgctcag
    19081 ggcgcagcaa cggtgtggat ggcctcacct gaactcgaaa actatgagcg caggctgcaa
    19141 caacttcttg atacctcccc ggtacagccg ctggaaaccg ggatgcagat gatgcgtgtt
    19201 gccgacagcc gctggccgga aagcctgcaa cagcaacagg cctcgacaca atggaatgag
    19261 gcactcaaaa cccgcgcaca gaatagcccg cagttgcgtg gctggttgca gacccgccag
    19321 gacttacatg cttttgcaga tctagtgatg cagcgcgaga aagagggact aaccctttcc
    19381 tatatcaaaa atgtcatctg gcaggcggag cggggactgg ggcaggaaac acccgttgag
    19441 tctctgttga cgcagtacca ggatgcccgt gcgcagaagc agaatacaga tgcgctggaa
    19501 aaacaaatta atgagcgact cgaaggcgtg ttaagccgct ggctgctgct gaataataat
    19561 acgataccga cgataaaaaa aggcattgaa tttcaataac attcatgaat acaaaggagt
    19621 tttaaatg
//
LOCUS       DAZKFU020000072        19520 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZKFU020000072 DAZKFU020000000
VERSION     DAZKFU020000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.072"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(444..1922)
                     /gene="wzxC"
                     /locus_tag="ACHOQQ_RS10155"
                     /old_locus_tag="pgaptmp_002031"
     CDS             complement(444..1922)
                     /gene="wzxC"
                     /locus_tag="ACHOQQ_RS10155"
                     /old_locus_tag="pgaptmp_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGU4470287.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVVVFFLSDAI
                     GDVLNNPDLAPLIKTLSLAFVMIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTVGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(1924..3318)
                     /gene="wcaJ"
                     /locus_tag="ACHOQQ_RS10160"
                     /old_locus_tag="pgaptmp_002032"
     CDS             complement(1924..3318)
                     /gene="wcaJ"
                     /locus_tag="ACHOQQ_RS10160"
                     /old_locus_tag="pgaptmp_002032"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGU4470288.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(3373..4743)
                     /gene="cpsG"
                     /locus_tag="ACHOQQ_RS10165"
                     /old_locus_tag="pgaptmp_002033"
                     /gene_synonym="manB"
     CDS             complement(3373..4743)
                     /gene="cpsG"
                     /locus_tag="ACHOQQ_RS10165"
                     /old_locus_tag="pgaptmp_002033"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU4470289.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTSLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(4936..6372)
                     /gene="cpsB"
                     /locus_tag="ACHOQQ_RS10170"
                     /old_locus_tag="pgaptmp_002034"
     CDS             complement(4936..6372)
                     /gene="cpsB"
                     /locus_tag="ACHOQQ_RS10170"
                     /old_locus_tag="pgaptmp_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGU4470290.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGELTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     TRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(6375..7598)
                     /gene="wcaI"
                     /locus_tag="ACHOQQ_RS10175"
                     /old_locus_tag="pgaptmp_002035"
     CDS             complement(6375..7598)
                     /gene="wcaI"
                     /locus_tag="ACHOQQ_RS10175"
                     /old_locus_tag="pgaptmp_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGU4470291.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQC
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAAERLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(7595..8074)
                     /gene="gmm"
                     /locus_tag="ACHOQQ_RS10180"
                     /old_locus_tag="pgaptmp_002036"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(7595..8074)
                     /gene="gmm"
                     /locus_tag="ACHOQQ_RS10180"
                     /old_locus_tag="pgaptmp_002036"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU4470292.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(8077..9042)
                     /gene="fcl"
                     /locus_tag="ACHOQQ_RS10185"
                     /old_locus_tag="pgaptmp_002037"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(8077..9042)
                     /gene="fcl"
                     /locus_tag="ACHOQQ_RS10185"
                     /old_locus_tag="pgaptmp_002037"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGU4470293.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(9045..10166)
                     /gene="gmd"
                     /locus_tag="ACHOQQ_RS10190"
                     /old_locus_tag="pgaptmp_002038"
     CDS             complement(9045..10166)
                     /gene="gmd"
                     /locus_tag="ACHOQQ_RS10190"
                     /old_locus_tag="pgaptmp_002038"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU4470294.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(10193..10741)
                     /gene="wcaF"
                     /locus_tag="ACHOQQ_RS10195"
                     /old_locus_tag="pgaptmp_002039"
     CDS             complement(10193..10741)
                     /gene="wcaF"
                     /locus_tag="ACHOQQ_RS10195"
                     /old_locus_tag="pgaptmp_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGU4470295.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(10757..11503)
                     /gene="wcaE"
                     /locus_tag="ACHOQQ_RS10200"
                     /old_locus_tag="pgaptmp_002040"
     CDS             complement(10757..11503)
                     /gene="wcaE"
                     /locus_tag="ACHOQQ_RS10200"
                     /old_locus_tag="pgaptmp_002040"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGU4470296.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSS
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRRLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTGKTKALYNKS"
     gene            complement(11629..12849)
                     /gene="wcaD"
                     /locus_tag="ACHOQQ_RS10205"
                     /old_locus_tag="pgaptmp_002041"
     CDS             complement(11629..12849)
                     /gene="wcaD"
                     /locus_tag="ACHOQQ_RS10205"
                     /old_locus_tag="pgaptmp_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGU4470297.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            complement(12824..14041)
                     /gene="wcaC"
                     /locus_tag="ACHOQQ_RS10210"
                     /old_locus_tag="pgaptmp_002042"
     CDS             complement(12824..14041)
                     /gene="wcaC"
                     /locus_tag="ACHOQQ_RS10210"
                     /old_locus_tag="pgaptmp_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGU4470298.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(14038..14526)
                     /gene="wcaB"
                     /locus_tag="ACHOQQ_RS10215"
                     /old_locus_tag="pgaptmp_002043"
     CDS             complement(14038..14526)
                     /gene="wcaB"
                     /locus_tag="ACHOQQ_RS10215"
                     /old_locus_tag="pgaptmp_002043"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGU4470299.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNSVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(14529..15368)
                     /gene="wcaA"
                     /locus_tag="ACHOQQ_RS10220"
                     /old_locus_tag="pgaptmp_002044"
     CDS             complement(14529..15368)
                     /gene="wcaA"
                     /locus_tag="ACHOQQ_RS10220"
                     /old_locus_tag="pgaptmp_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGU4470300.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(15461..17623)
                     /gene="wzc"
                     /locus_tag="ACHOQQ_RS10225"
                     /old_locus_tag="pgaptmp_002045"
     CDS             complement(15461..17623)
                     /gene="wzc"
                     /locus_tag="ACHOQQ_RS10225"
                     /old_locus_tag="pgaptmp_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGU4470301.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAYRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(17626..18069)
                     /gene="wzb"
                     /locus_tag="ACHOQQ_RS10230"
                     /old_locus_tag="pgaptmp_002046"
     CDS             complement(17626..18069)
                     /gene="wzb"
                     /locus_tag="ACHOQQ_RS10230"
                     /old_locus_tag="pgaptmp_002046"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGU4470302.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(18075..19214)
                     /gene="wza"
                     /locus_tag="ACHOQQ_RS10235"
                     /old_locus_tag="pgaptmp_002047"
     CDS             complement(18075..19214)
                     /gene="wza"
                     /locus_tag="ACHOQQ_RS10235"
                     /old_locus_tag="pgaptmp_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGU4470303.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         4550 a         5429 c         4881 g         4660 t
ORIGIN      
        1 gccggataag gcgttcacgc cgcatccggc attcagtgcc tgatgcgacg ctggcgcgtc
       61 ttatcaggcc tacaggtccc gagcacagaa ccgtaagacg gataaggcgt tcacgccgca
      121 tccggcattc agtgcctgat gcgacgctga cgcgtcttat caggcctaca agatccaagc
      181 aaagaaccgt aggatggata aggcgttcac gccgcatccg gcattcagtg cctgatgcga
      241 cgctggcgcg tcttatcagg cctacaagac ccgagcacag aaccgtaggc cggataaggc
      301 gttcacgccg catccggcat tcagtgcctg atgcgacgct gacgcgtctt atcaggccta
      361 caggtcccga gcacagaacc gtaggatgga taaggcgttt tacgctgcac ccggcaaccg
      421 ttgtcggagc ctaaaacaac cattcacccc gcccgtaaaa gcattttcat tttttcgctg
      481 cgacaaaact gacgcttcac ttccaccacc agcggatggc gcgacagcac aatcatcacc
      541 acaaacgcca gcacgcccgc ggctatttgc accgccagca gcatccccag cgccagttgc
      601 cctttcagca caatgcccag cgcataactg accaccagcg tcggcagaga gagataaaac
      661 ggcagccaca aactcaggat gtactggcga taactggaac cgagcaccgg tttaatcatc
      721 acgaagtaac tcagaatggt gttgataatc tgcaccagca ggaagccaac cgtgacgccg
      781 attgcgcccg ccatctgccc accaataaca atcgccggaa taaacagaaa tgttttgaat
      841 acgttgaatt taaagctgat atcgacccgc gctttcgcca tcagcagcga accaatcggg
      901 ttacccaccg aacgcagcag acccaccaca cacagcaatt gcagcaccgg aataatgctg
      961 ttccacttct caccaaagac cagcggtaca aagttattcg acaccaccat cagccccagc
     1021 agagccggaa agttgataat ccccactacc gacagcagct tgtagaagtt aacgcgcagc
     1081 ttttcggtgt cgtcctgaat tttggcgaat gccggaaaca acacgcgggt gatgattggg
     1141 ttcagcttca tcggtggcac aacggccacg ttgtacgcca ggttgtatcc ccctgccacg
     1201 cccgcgccga gaatacgcgc cagcaccagc gttgaaaggt tggtattgag atagttgatg
     1261 atgctgtccg ccgtcagcca ggcaccaaag cgtaagttcg gtgccaccga cgccagcgag
     1321 aaatgcagac cggggcgata aattttgcgg ccaaagtagc caaacagcag cgttcttacc
     1381 gcactattga ccagataacc gaggatcgcg gtcatcgcca gcggccagaa atgggcgcta
     1441 accaccgtaa aagtgaagcc cgccagtact gcgctggttt cgatcatgcc gattttgttg
     1501 aactccagct ccttctgcat cagtgcgcgg aactgctgcc cgtgggggat catcacaaac
     1561 gccagcgaca atgttttaat caacggtgcc aggtccgggt tattcaacac gtcaccaatg
     1621 gcatcactca aaaaaaacac caccacgcac accacgatcc ccagcccgac gttcagccag
     1681 tacaacgtgg tgagttccag gtgactgatt tcttttcgct gaataatcga gttagcgata
     1741 ccaaagtcag aaagcgtatc tgccagcgcg ataatcacca gcgacacggt cagcaaaccg
     1801 aactggtggt tgtcgataat ccgcgccagc acggtcatct gcaccagccc gaggccgata
     1861 atgatcaccg tggcaatcgc cgaccacttc gcgccgctga tggttttttc acgtaagctc
     1921 atatcaatat gccgctttat taacgaagcc cttgaatacc gtcaggaaaa cgattttgat
     1981 atcgaaccag acgctccatt cgcgaatgta ctcaaggtcg aattcgacgc gtttttccat
     2041 tttctccagc gtgtcggttt cgccgcgcca gccattaatc tgcgcccagc cagtaatgcc
     2101 cggtttcacc ttatggcgca gcatgtagcc ttcaatgagc tggcgatatt gttcgttatg
     2161 cgccactgcg tgcggacgtg gacccacaat cgacatcccc ccggtcagca cattgataaa
     2221 ctgcggcagt tcatccagcg aagtgcggcg cagaaagttc cccactttag taacgcgcgg
     2281 atcgttctgc gtcgcctgag tcaccacttt gtcgttctcc atcactttca tggaacggaa
     2341 cttccacact ttgatcggct tgccatccat gccgtagcga gtctggcgga aaataaccgg
     2401 ccccggtgaa ctcagtttca ccgccagcgc aatacagcac agcaccgggg aaatcagcag
     2461 taaaatcagc gttgccagca caatgtcttc cgcacgtttt agcaggcggt taaccccgga
     2521 aagcggcgtg tcgtacagcg gcaccacagg tacgccgttc atctcttcga tacgggaatg
     2581 aagaatattg aaggtaaaaa catcggggat cagcagcacc gaacaggtgg tatccgccag
     2641 ttgatggacc agttttttca ctcgcgcgcc gtcgcacatc tgcatcgcga tatagacgtt
     2701 atgaatcttg cccgcttttg catcctcgac cagctgttgc aggttacccg cccagtcgtt
     2761 agaaacgccg cccggttttg ggtcgtggta tacacccacc acttcaaacc ctaaccacgg
     2821 ctggttacgg aagctctcca tcagcatttg cccggcggct aaatcccccg ccaccgcgac
     2881 catgcgcttg ttatagccat gattacgcag ccagcccgcc ccaatgcgaa tacacgaacg
     2941 gcaaaccacc agtccgatgc tggtcagccc ataccacgcc agccagattt tcagttgcgt
     3001 gtcgaaatca ttgttgaacg ccaccagtcc ggcgctgaaa atcacgctta aggtccagtt
     3061 ttgtagcaac agggcaaatt ctgtcgctgc ccgaacaccg cgccatgagc gataaaaatc
     3121 ggtgatgcca cccagcatct ggaacaccac cagcgtaatc agcgccacca acaggtgcat
     3181 gtagaggaat gacagtccgc tgacttcgca aaccagccat agtccggcaa acatgatggt
     3241 gatatctgaa aagcgttgca ccatagagat taacgatgca ttggttttcg ctcgctcgcg
     3301 cttttttaga tttgtcatcg ttgttcctgt tattagcccc ttacccgcag tggggtaagg
     3361 gaagatccga cattactcgt tcagcaacgt cagcagagtt cgcgttcgcg cttccatcag
     3421 cggcacatca ccgcgcgatt ccacattcaa ccgcaccacc ggttcggtgt tggaggagcg
     3481 caggttaaag cgccagtcgg caaaggtcat gctgatgcca tcggtgcgat ccaccgccag
     3541 cgcctcacgg ctaaaatgct gttcgacgcg gttaatcgcc tcaacggggt gcgccagttt
     3601 gctgttgatc tcaccgcttg ccggaaacgc cgccatccgg tcgcgcacca gttcgcccag
     3661 cgtttttcct ttcaggcaca ccagttcggc gaccagcagc cacgggatca tgccgctgtc
     3721 gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt tcgccaccgt agatggcgtc
     3781 ttccttgcgc atccgttctt taataaaggc gtgtccggtt ttcgacatca ccggcgtgcc
     3841 gccagcagca gtcaccacat caacagtgtt ccaggagaga cgtggatcgt ggatgatctt
     3901 cgcgccggga tttttttcga ggaacgcttc tgccagcagg ccgacaatgt agtagccctc
     3961 gataaactgc cctttttcgt caaacaggaa acagcggtca aaatcgccat caaaagcaat
     4021 gcccatatcc gcgccgtgtt taatgaccgc attgcgggtg tcgtcgcggc attccggcag
     4081 cagggggtta ggaataccgt tggggaagtt gccgtccggt gtgttgtgta ctttgattaa
     4141 ttccacgggc gcgccgaggg ctttaaagcg ggcttcaatg gcgtccacca ccggacccgc
     4201 cgcgccgttc ccggagttga tcaccagctt gagcgacgtg aggtttttga cgttgatata
     4261 accgaacagg tgatcaacgt aagcgtcacg caggttgatt tgctgatagc gaccgcgttt
     4321 ggtttcatcg acgggaggaa agtcgttggc ttcagccagg cgctggacat cacgcagtcc
     4381 ggtatcaccg ctgatcgggc gagccccttc gcgcaccagt ttcatgccgt tataatccat
     4441 cgggttatgg ctggcggtaa cttcgatgcc gccatccacg ccgagatgga acgtggcgaa
     4501 ataaatctct tcggtgccgg acatgccaat atccagcaca tcgacgcccg catcctgtaa
     4561 acctttcgcc agcgccagtt ttaaggtttc gctggtgagg cggacatcgc cgcctaaaac
     4621 aatggttttc ggtttaagaa attcgccata ggcgcgacca atacgccagg caatatcttc
     4681 attcagttct tcgcctaatt tcccgcgaat atcataggct ttaaagcagg ttaatttttt
     4741 catatcgtta ccctttttca ggcaatagtt ggccctgacc gaaacggcca attttgtttt
     4801 tgtcgttatt tacaccgcgg tttcgcattc attgcctgat gcgacgctgc cgcgtcttat
     4861 caggcctaca aaaccgaacc gtaggttgga taaggcgttc acgccgcatc cgacaaacag
     4921 cgcctgatgc gacgtttaca cccgtccgta gcgatccgcg aagcgcacca catcatcctc
     4981 ttcgagataa gagccggagc gcacttcaat taaatcgagc ggaattttcc ccgggttttc
     5041 caggcagtgc gtcgccccca gcggaatata aatcgactcg ttttcaccaa gcagtttgat
     5101 atcaccgtca atagtgactt ttgccgttcc cgcgacaacc acccagtgtt ccgcgcggtg
     5161 atggtgcatc tgtaccgaca agccttcgcc cggtttcacg gtgatgcgtt tcacctggta
     5221 gcggtcgccc gcgtcgatag agtcatattt gccccacggg cgatagactt cgcgatgtac
     5281 ccgatgctca tggcgaccat cggctttgat ctgctcgacc acttttttca catcctgcac
     5341 cgcgttacgg tcggcaatca gcaccgcatc tttggtctgc accactacca gatctttcac
     5401 cccgacggtg gttacgaggc cagattcggc atacacatag ctgttttcag ttttgtgatt
     5461 aatcacatcg ccgtggcaaa cgttgccctc ggcggtgtgg gcgctgatct cccataatga
     5521 agaccaggaa ccgacatcgc tccagcccgc atccatcggc accacaacgg catctgccgt
     5581 acgttccatg accgcgtaat ccaccgactc ttccgggcag gccagaaacg cttcttcatc
     5641 cacgcgaata aaatcgagat ccggatcgac ggcactcatt gctttttcac aggcatcgag
     5701 aatatccggg cgatattttt tcagttcttc gagatagcgt ccggcgcgga acaggaacat
     5761 gccgctgttc cagtaatatt cgccacttgc cacataggcc tgggcggttt ccagattcgg
     5821 tttttcgaca aactgcgcca cttcaaaggc caccgtatcc tgctcgcccg cagatacttc
     5881 accgcgacga atatagccat atccggtttc aggtagatcc ggcacaatgc cgaaggtcac
     5941 cagtttgccc gcttcggcat atggcatggc attacgcacg gcggcacgga acgcgtcttc
     6001 atcggcaatc acatgatccg ccgccagcac cagcattaac gggtcgctct ccggactatg
     6061 acgtgttgcc gccagcgccg ccagcgcaat ggctggtgca gtgttacgcc ctgctggttc
     6121 gagaataatg ttctcggtga gtttgttcag ttggcgcagc tgttccgcga caataaagcg
     6181 gtgctgctca ttgcaaatca ccaccgggct ttcgcactcc acgccgttca ggcggcagat
     6241 ggtggtttgc agcatggtga gttcgccttt caggcataaa aactgcttgg gataaagtac
     6301 gcgggaaagc ggccataagc ggctaccgga gccacctgcc atcacaactg gatagagttt
     6361 cgactgcgcc ataattatcc ccgaatatca tttataaatt gacgtaacac gttctcttta
     6421 tcgagcgtgc gttcggcata ttcacgtgcc accgtgttgt gtttgggcag caggagcgcc
     6481 tgacgtatcc ccgccaccag cgcctcgacc gattccggtt ctacgcaaac cgcaatgccc
     6541 ggaaaggttt cgcaaagctg tcccagttct gtgtgggcct ctgcggtaat caccgcgtta
     6601 ccgcctaccg ccagaatatt ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt
     6661 ttttgcacca ccagatggca atcgcccatc ttcagcagtg cgggtaaagc gtcatacgat
     6721 tgcagcggga agaattgcat gttgtgcagt ccacgctgct gcgccatttt ttccagccgc
     6781 gctttgccgc cgccctgccc gacaatggca aaaatcagcg gttcatcacg caggcgttcg
     6841 gcagcttcaa taacgttttc cagcccctgc ttttcaccaa tattgccgga gtaaagaatg
     6901 atttttttgt tatccggcag gccaagctgg ttacgaaggg catcaacatc ggcgtctgca
     6961 acatgctgaa aacgggcgat ttccgaccag ttggggaaga aaatgacgtt atccgccgcc
     7021 acgccttttt cgatggcttt attcatcatc gaacgcgaaa tcgtggagac gttatcgacg
     7081 ttatgcagtc cgctacgttc gaacgccgtt gccagctgtg ccactttgcc gcctttgcct
     7141 tttccggcaa ggcccagccc cagcatggcg tccacttcgt aatcctgaat atgcagcacg
     7201 gtacgcgcac cagagagttt cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc
     7261 actacgccga taatgcgatc cggcttccag cgacattgcg ccatcaacgg gaaaaaactg
     7321 ctgacggcaa aactgccgag atgtaacagg cgtttcaggg tgctcggctg ttttggcacg
     7381 tacagcgggc agcgccacac cgtagcggcc ccctcttctc gtttgtagcg ccaggcggaa
     7441 tagttctcgc ccacctgcca ctgcgggtag taaggcggtg cggtaatgac ccgcacctca
     7501 tgaccttgtg ccgccagcca ttccaccatc tcgccggtgt atttgccgat gccggttaac
     7561 tccggcgagt agttaatgcc gtagaccagt attttcataa tccgggtact ccggcacgct
     7621 tctcagcgag aaaataggcg cggctgttag cgtgaacatt gtcactggcg agcagcgcgt
     7681 ccggcgtcaa ccagcggtaa tcgtcatgct gctcatccgg cagtaacagt tcttcttccg
     7741 ctactctgaa gcgaaaaccg agcaccacat agtgagtggt gaaatccgtg ccagagaagt
     7801 tatcgtcata aaagtgctgc cagacaccgt aaaactggcc tgctgttatc ggcagacgca
     7861 gccccagttc cgccatcgtc agccgctcaa atgcggcttc cagcgtttcg tctttctgca
     7921 cgcgccctcc cggcacaaac cagtaaccct gcgccgggcg gttggttctt ttgccaagca
     7981 gaaactcgcc gcgactgttc tcgacaataa agtcgagaga gacaagcgga gtggagcgca
     8041 ctaccgtggc aaagtcttcc tgacgtaaaa acatcattac ccccgaaagc ggtcttgatt
     8101 ctcaaggaac cactggtaag tgctggcaag ccccgcttcc agtgagattt cgtgatacca
     8161 gccaagctga tgcaggcgcg tcacatccag cagtttgcgc ggcgtaccat ccggtttact
     8221 ggcatcaaaa accacccgac ctttgtaacc caccactttg gcgatggttt gcgccagctc
     8281 gcggatagtg cagtcaacgc ccgtgccgac gttaatgtgc gacagcatcg gctgggtgtt
     8341 ctccagccag acttcgtgcg ccagctccat gacatgaatg ctcgccgccg ccatatcatc
     8401 gacgtgcagg aattcacgca tcggtgtacc gctgccccac accaccacgt ccggtgcatt
     8461 ctgtgccgtc gcctcgtgga agcgacgcag caatgcaggg atcacatgcg aattactcgg
     8521 gtggaagttg tcatgcgggc catacaggtt ggtcggcatg accgagcggt aatcgcgtcc
     8581 atactggcgg ttgtaagatt cgcacagttt gatcccggcg attttggcaa tggcgtaagg
     8641 ctcgttggtc ggctccagcg tgccctgcaa taactcgctt tctgccatcg gctgttttgc
     8701 cagtttcggg tagatgcagg acgatccgag aaacagcagt ttgttcacgt cgttctgatg
     8761 cgcggcgtga atgatgttgc tctcaatcat catgttctga tagatgaaat ccgccggata
     8821 cgtgttgttg gcaacaatgc cgcccacttt cgcggccgcc agatagacct ggtcaatacg
     8881 ttcgctggca aagaaatcat gcacggcgcg gctgtccagc aggttcagct cgtcgcgggt
     8941 gcgtaatacc agttccacat caccgcgctg ttcgagctgc cgcctgatgg cggaaccgac
     9001 catcccgcga tggccagcaa taaaaatgcg ttgtctactc atacttatga ctccagcgcg
     9061 atcgccacgt cgtagccgtg agatttcagc agagagtgtt ttttcgccgc ttcgaggtca
     9121 ttagccacca tttcagacac catctctctg agggtgattt ccggtttcca gcccagtttt
     9181 tcgtgcgctt tggtcgggtc gccgagcagc gtttcaactt cagccggacg gaagtaacgc
     9241 ggatcaacgg cgataatcac atcacccggt ttaacgcccg gcgcgtcatg tccggtgacg
     9301 gaaaccacaa tgcccttctc ttcaacgccc gtgccttcaa agcgcagttt gatgcccagc
     9361 tgtgccgccg ccatttccac gaactgacgt acggagtact gaacgccggt agcgataacg
     9421 aaatcttccg gttgttcctg ttgcagcatc atccactgca tttttacgta gtctttggca
     9481 tggccccagt cacgcaggga atccatattg ccgaggtaca ggcacgattc cagcccctgg
     9541 gcgatgttgg cgattgcgcg ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg
     9601 gattcatggt tgaagagaat accgttgcag gcgtacatgc cgtaggattc gcggtagtta
     9661 acggtgatcc agtaggcgta cagtttggcg accgcatacg gagatcgcgg gtagaacggc
     9721 gtggtttctt tctgcggaat ttcctgcacc agaccataca gttcagaggt ggaagcctga
     9781 tagaaacggg ttttcttttc cagaccgagg aagcggatcg cctcgagcag gcgcagcgta
     9841 cccatcgcgt cgacgtcagc ggtatattcc ggtgactcaa aagagaccgc aacgtggctc
     9901 attgccccca ggttgtacac ttcatccggc tgtacttcac gcaaaatgcg cgtcaggttg
     9961 gaggtatcac tcaggtcgcc ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc
    10021 tgataaatgt gatccacgcg ctcggtgttg aatgacgatg cgcgacgctt aataccatgc
    10081 acctcgtaac ctttttccag cagaaactct gccaggtaag aaccgtcttg tccggttaca
    10141 ccggtgatga gagcgacttt tgacatgtct tattcctctg tattttttga atttattcag
    10201 tttcaacgcg ttcgcgtatc accactgcgg gattcccccg gcaaaccaca tttgccggaa
    10261 gcgatttaaa aacactgctt cgtgcaccca cgacggtgcc gtcgccaatc gtgacgccag
    10321 gggcaacaaa aacatctgtc gccagccagc atttctcgcc aatcacaata ggcgtggcgt
    10381 taatggtgaa atgttgactt gcatggtcgt ggctaccggt gcataaataa cttttttgcg
    10441 atatcaccga atgtgcgcca atggttattt caccgagggt atataaattg acgtcatcgc
    10501 cgacccacgc gtaatcacct aaggttaatt tccacggata ggtaattttt actgacggac
    10561 gaataactac gttttttcct atttttgcgc cgaataaacg taataaaaaa gcccgccagc
    10621 gatacaatac ttgtggcgac caggcaaata ttgttgcctg tactgcccac cataattgca
    10681 cttttatagc gttgccgccc cggaaccctt tcggcaccga gaatccactt aaatcttgca
    10741 tcgttttccc ttatattcag cttttgttat ataaggcttt agtcttacct gtcgtacgct
    10801 ggcgtaaatg ccaggataat tcagcccaga agccaggcac atgtaatatt tgacgttgga
    10861 cttttttggc gtcagcacac aattccatat tattggtggt agatacccca cccatagaaa
    10921 attcagacac cagtccattg agttttttaa atgcataacc ggctttatac attttggctg
    10981 ccagcgcgta atcagaagaa actttatatt ccaggtcata acgccatttt ttcaagccgg
    11041 ataccgggaa aaatatcgcc tgatgactgg cgggcagact gtgataaata taccaacccg
    11101 gtttggcgct acgtttaatt ttatgcccgt cgccaaaatc cagtagcgca tcgccggtga
    11161 tcatcacgtt atctttttgc acttttaacc tacggacaaa atttgcggca tcctgatgaa
    11221 aaatatcacc tgagttgaga aacaacgcga acttaccttg cgccatcgca atgcctttgt
    11281 tcatggcgtc gtagataccg ttatctggct cgctgacaaa gcgtaggtta tagataccat
    11341 tgagattttc caggtactca cgggtgccgt cactggaacc gccatcgaca acaatccatt
    11401 cgaagctgat atcgtccgcc tgcgccagat gcgccagcga ggcgtgtgtt ttgactatcc
    11461 cttcgaggtt acgaaacgcg acagtgatta tgctaagcaa catgttttac ctcgtaatac
    11521 ccgtcatact tcaagcggca tgtgcgttgg ttttcctcgc tcaccccagt cacgtactcg
    11581 tgtacgcttc tggggattcg ctgcgtcacc aacttcctgc aactcggatt atttagggtg
    11641 tatatttaac gcttttcgca aaataaacgg acagacgatt aaaaatgcat attccgggct
    11701 aaatatcgaa ccggtaaaaa acagcgatac cggagtaaaa agatataatt gcacgcgaaa
    11761 attgcggtta tcaccaaaag cgttgatcat catttttatc actttcccca tgtaccacag
    11821 cgataaaaac accgcgaacc aggaaaaata aataatcagc agatacaaac cattgtctat
    11881 ggtttttccg acatccgcac cgttaaatat tccgaatgat gcgacatatt cataaagtga
    11941 gccaaatctg actacaccgt caatatgggt caaagaataa ccgaccatca ccagcggacc
    12001 gacaatacga taatacgatg acgatccttc cgtacctaaa tcgcccagac gggtggaaat
    12061 ataaggaaac gcgattacca caccaaccag gaatacagcc agagaaatca atgctaacgg
    12121 taactttttc ttaatcgcct ctttattcag atattgaaat gcccactcca gcaaataaaa
    12181 caggataaag gtcataaccc ctgaaaacga tccggataat attatccctg cgagaatcat
    12241 agcatcggtt ttaggcgttt tgataccaaa ctgtttgatg ctgagccaaa ttgagattaa
    12301 tgccagagcg aaaaatgccg gttcgaaata aagtgctgtc gtgcgcttgc cgccgaattt
    12361 aatgaaattc agcacatagc tgttgctgta aatcagatat ttcgaaatcg actccataat
    12421 actgctgcca ccggtgagga taatttgcgc catctccacc gccgccagcg ccaccaccag
    12481 cccgaccacc agataaaaga aacgtaatat cttgcgatgg ttgtgtggcg aaatcgtttt
    12541 aaagcgaata ctccacacca tgccaataat gatcacaata tagacaaaca gcatggttga
    12601 agtgacgtat ttactggcat ccaacgactg accaaacagg tagttaaacg ccgtgagtcc
    12661 cgcgccaatc cctaaggcaa tcatcaattt tttaacgctg atgcgttcta aaaacagcag
    12721 taacaagaca ggtaaaaaag tgacgatggt gatggggaaa ctttcgccaa gctgagcgat
    12781 tttgacgtta accagcaggt agatcagcgg cagcagcagg tagctacaga ttctgataga
    12841 agttgacata ctcctccagc atctgttgtc cactgtaggc ggcgcggctg cgttggctga
    12901 actcagccag cgtggtacca aatatcgcct gcgcgatttc cggtttgctt aactggacca
    12961 gttgcagcac ctcttcttcg ctgacggttt taccgccgga tttttgcaac acttcacgcg
    13021 ccgcgtcgct atgggtggca atcaccggca cgccaattga taacgcctca cacaaaatca
    13081 gcgggtagtt atcgacgcga gaactgaata ccagcgcatc catctgattg agcgcgctca
    13141 tcaacttgcg cttgtcagtt tcaaagccgt gattaaccac gttgccagcg gtgaacggcg
    13201 agaacttacc aaaggtatgc agttcgattt tgtcgcccag cgccatcatc tcgcgtacca
    13261 gttgctggtt agttttgccg tcgtaacgca ggtcatgcgc taccaccgcg attttcggct
    13321 taccctgagt ttcgcgcacc ggaggcaaat ccgccagaat cgcctcggtt gccatatcaa
    13381 taccgttatt gataatccgg caacgccctg gaccgtacag gctattgaaa gcgtcagcca
    13441 catgctggct gggggaaata aactgacagc ccagcgccag catctcacgg aataactggc
    13501 gtttgcccgc caccagctgg tgcgcccgat caatcttcac cggcggatag ttatttaagg
    13561 tcgggcattt ctggcagccc gttttccagc cttcgcaacc gtcggtaaag gcgcagcgtc
    13621 cggttacact ccagtggtcg tgcagcgtcc agaccagagt gacgtccggt ttgtggtttt
    13681 tcaccttttc gcaaaagcgc accacgctct taagatttag ccagtagctg tgcagcacat
    13741 gaaaatgaag gaccaccgga cccggtgtgc gagtaatggt gcgatataac tcattgaaat
    13801 tgccaaacag atcgcgatta aacagacgaa acagggcaat attcgccatt gcggtcatcc
    13861 gcggcgtatg tttgattacc tgcggatagt tctgatggct gacgctctct ttgccgcctt
    13921 tgccgtagcc gtagacaaaa tgtgacgcca gcccctgttg cagcgcgcgc tggtggagat
    13981 ctaacgccac acctgctgcc ccgccttccg ccagtcgcac attaaattgc agaatattca
    14041 tttaattacc ttcactcgcg ctttttctcc caccaccagc gcgttgtccg ggacagagtc
    14101 gagcaccacg ctgcccgcgc ccacggtgac gttgttacca agcgtgatat caccaagaat
    14161 aatgacgttg gcaccgagtt cgacgctgtt gccaatgtgt ggacatgcca tgttatcggc
    14221 accacgattg ccgatagtga cgccgtggcg aatggtgaaa tcatcccccg ccaccacgtt
    14281 tttattgatc actacggcgt aaccgtgatg gatagtaaag cggcggccaa tggtcgcggc
    14341 ggcctggatt tcataaccga aaaagcattc ggtgataatg cgatacagca ccagcagcgg
    14401 ggccgcccac agattgttga ggacgttctt tttgcgccac accgaacaaa aatgagcgac
    14461 gcgataggca agaaccatgc agcacgggcg taaactccag ctgttggcgc gtagatcttc
    14521 cagcatattt agcgcccccg aataccatca gccagacgtt tgccgttacg caccgacagc
    14581 agcgtcagca aagtgcgcca ggtcatacgt ttattgcgga tctggtagag agtaaacagc
    14641 tgatattttt tactggcacg gtcgaatttg tctttgtgct tgcggtaaaa gtggaagtat
    14701 ccggagaatt ttttcggtga cgaggtgatc tgcatttcac cgtgattgat atgcaggatc
    14761 tgcgtcgctt cttctacttt ccacggttcg ccatactcca ccaccatccg caggaagatg
    14821 tcgtaatcct gtgccgcttt cagttcggta tcgaacaaac actctttgaa acgccacgcc
    14881 caggtaaaga cctgattgcc aatgatattg cgtttataga acaggcggcg tgagtacggc
    14941 gatttgggat acagtggtaa gcttgccggt tgggaataaa cttcgccctg gcaaacgtaa
    15001 tcgttagcgt acaaaaaggc gtgagtgacc agttgctgtt tatgggcgag gaagacgctc
    15061 agacggttgg gtgtccattc gtcatcgtca tcgatcccgg taatgtattc cccttgcgcc
    15121 agcataatag cctggttacg taccgcgcac gccccgctgt taatgtcgtt gtgaatgtag
    15181 gtaatacgcg gatcgttgag ggcggtgacg tactgttgca gttgttccca agaggtggag
    15241 caatcatcca cgatgatcat ctcccagttg ctgtagtcct ggcgcaagac cgattttatc
    15301 gcccgaatcg ccagttgttg gcggttccag gtcggcatat agattgagat cagcggattg
    15361 tctttcatag gttgttctcc cggcttatga gctggtttgc ttgccggatg cggcgtgagc
    15421 gccttatccg gcctacgggg cggtgcgaat gcaggcgtcg ttatttcgca tccgacttat
    15481 attcgtattc gtaatagcca taatcctgat acgcgctggc gcggcggaag atggagttca
    15541 gaatcacccc tttcaccgga ataccgtttt gctcaaagcg gctcagactg gtttccactt
    15601 ctttcaatgt gttgaccgca taacgcgcca ccattaacgt ggttccaaca tgacgaccaa
    15661 caattgccgc atcggtcact gccagaatcg gcggcgtatc aatcaacacc aggtcatagt
    15721 ttttactcgc ccagttcacc agttcggcaa agcgttcgct catcaacagt tcagaaggat
    15781 ttggcggcac ctgaccgcgc gggatcaggt caaatttggc aatagaggtc ggtttagctg
    15841 cggtagtaat atcgccctga ccaatcagaa tttccgacag gccattgacg ttattggtgc
    15901 ccaacagctc gtgggtgtag cctttgcgca tatcgcagtc gatcaacaac acgcgtttat
    15961 tggtctggct gatcaccgcc gccaggttgg cgcagacaaa ggttttaccg attgacgggc
    16021 taaccccggt catcatcaac acattgttct gcgcctgcat catcgcgaag tgcagactgg
    16081 tacgcagact gcggatggct tcaatcgcca gatcggttgg attccccacc gccagtagct
    16141 ggctctgttt atagcgttta actcctttga tggttttgac gctatcgcgc gctttctgcc
    16201 actccgacag cgggatgctg gcatagacgc tgataccgtg ttcttccagc acctgcgggc
    16261 tttcgatgcc gcgattaaac aacgagcgca gcagcacacc cacgatagag agcatcaggc
    16321 cgaggataat cgccccaagg ataatcagcc ctttcttcgg ttttagcacg ccaggctggg
    16381 tgattgccgg gtcaacaatg cgcacatcac cgacggtgct ggcctcggtg attttcagct
    16441 cctgctcttt attcagcagt tgcatataga cctgctgacc agactcgaca tcgcgggtca
    16501 gacggacaat ctcctgctgg gttttcggca tcgccgttac gcgaccgtta agtttggctt
    16561 tttcgtcttc cagcgcctga cgtttctcca gcagcgtgcg gtacgcgggg tgaactttgg
    16621 tgtacagctt ggagatttcc gcctctttaa aggtcagttc gttcaactgc gcgtcgatgt
    16681 tcaccatcga atcgagcacc gctttcgctt ccagcggcag atcaacagaa tctttatcct
    16741 gacggtaggc attcagtttg ttttcggcaa catcaaggcg gctacgtact tccggtaact
    16801 gttgcgcgag gaaagcgagg cttttcgacg cttccgccga tttgcgctca atattttgtt
    16861 cctgatagtt acgggcgatg ctgttaagaa tgtcgcggat ctgttcgcga tcttcaccgg
    16921 tataagtcag gctcagtacg cctgcgtctt tgccgttctc cgttaccgtc aggctgtttt
    16981 gcagttggtt gatcatcccc agcgtggagt atttggtgac ggtaaactca ctgcccgggc
    17041 tggcgtgaat agcttcaacc atcagcgtga cgccttcttt tttcagcatc tggcccgctt
    17101 gtccacgggc gctaaagccg ccatcactgc tcagggtgta gtttttgtca tccagcacat
    17161 taagcgtaaa cacctgatcc gccatctctt tcggacggtt aaaggtagtc actttcaccg
    17221 tctcgttctg acgtcccatc aggcgatccc agcccgcacc gaaaatcggg aacgtgtttt
    17281 tgctcaccgc aatatcgagg tcgagatcgt ccaccgtttt accaagcacc aggcgcgaac
    17341 gaatcaactg gatctcggcg tccgatgcag gcggtttgtt ggctaacgcc gatccgatat
    17401 cctgcactaa cgaattgccg ctgctttgct cgatttgtac cagtgcgtcg gcactataaa
    17461 tcggcgtggc gaagaaggtg taaaccacgg cacaaagggc gaatacagcg gtgatgccaa
    17521 tcacccacca gcgcgcttca atgacggtgc cgaccaggcg accaatatcg atttcatcac
    17581 tgcccgttac cggagcggca tgttgtttta ctttttctgt cattcttata cctgctctgc
    17641 gttcaatgcc tgcgcccact ggcgggcaga ccgttcaagt aatgtgtaca ccgctgcaaa
    17701 cgtttcccgg cttttgcgat acggatcggg gatttcacat tcgttatccc agtgaccaaa
    17761 cagcatcact ttgccgcgca tctccggtgc catctcgcat aagcgttcga tatggcgctt
    17821 ttccatggtc aaaatcaggt catagttgcg acacaggcgg cggctgattt gtcgtgcaca
    17881 gtggccttcc agagacagtt gatgttctgc ggcgacgctg atagcggtag gatcagcgcc
    17941 cttaccgacc agcgcgccga gtccagcgga ctccactttt agctccgggt gataacgttg
    18001 cagtaagcgt tccgccgtcg gggaacggca aatattgccg acacagacaa ctaagatgtt
    18061 gttaaacatg acgattacca gttatgaatg tcgctggctg tatccgtcat gtaacggaca
    18121 ccgctaatag ttggcagcaa ctgattgatc agacggttcc agcgggaaac cggggcggtg
    18181 gtgacataca ccacgtcata aggttgcagg cggaattctg tcgccatcac cagcgacgtg
    18241 gcatcggaca tatccagctg gtagatattg gcaatcttgc cgttacgccc gccctcgcct
    18301 ttcagcggac gaatgacaaa gatgccgctg gcgttggagg tggtcatgtc aatgccttca
    18361 gcattgccca gagcttcagt cagggtcatg ccgctaaagt ccattttcag ggtgctctgt
    18421 ttcttcactt cacccatcac aaatactttc agatcatcat tacgcggcac gtagagaata
    18481 tcgccggggt aaagcaggcg attctggttg aggtcgccgt tttgcatcag cgcctgcaaa
    18541 gaaatgcgct cttcacgacc attgtgtgtt agcaccacgt tgcgccagtc agcggtgtcg
    18601 gtcaggccac ctgcggcgtt gatggcgtcg agaatggtca gtggtacgtt ggtgatcgcc
    18661 tgttgaccag atttattcac ctgacctgat atataggcct tttgtgagcg gaaggcggcg
    18721 atattaacgt ccacctgcgg gtcagcgatg tacgtcgcta agcgcccggt aatatcactg
    18781 cggatttcag cgagcgtttt cccgactacg tgaaccttgc cgatatacgg gtaaaacata
    18841 gtgccgtcag gctgtaccca gttgccggtg tcgctggagc tgcggtactg accggctggc
    18901 gtggtgagtt ccgggtgatc ccagacggtg acattcagaa cgtcgcccgg cccgacgcga
    18961 tactggtaat tcgcgatctc actttccagc gtcatattgg ggcgcgctac gttgggtcgt
    19021 ggacgtaatt gatcaatcag gcgcggggtc aacggataaa cattcaccat tttgtcgaga
    19081 tcgaaatcag cgtcctgctg tttgatgacg tctttgccca tcgtcgacat attgctgccc
    19141 ggaagtactg tgcaaccgct tatcaaggtt actgacacca ataatggcat caatttcatt
    19201 ttggatttca tcattgttta tttatcactt tggcagagta attatcctgt gcactattaa
    19261 tagcaatgtc gccatgcaca tttaccttgc agttaattga ataaaaattt aactggcatc
    19321 agtcctaaaa aaattgattt catccgcagg ctattgacag aataattcag actggtcttt
    19381 caggcatcca gacacgctac cgcccctggc tttttagcta ccaatacact gatttagctt
    19441 aatttttaaa accctctcaa catacagttg ttgatgagaa agggttattg cggaaattaa
    19501 cttccgaata taaggtgaca
//
LOCUS       DAZKFU020000073        19417 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZKFU020000073 DAZKFU020000000
VERSION     DAZKFU020000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.073"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(56..640)
                     /gene="pth"
                     /locus_tag="ACHOQQ_RS10240"
                     /old_locus_tag="pgaptmp_002048"
     CDS             complement(56..640)
                     /gene="pth"
                     /locus_tag="ACHOQQ_RS10240"
                     /old_locus_tag="pgaptmp_002048"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGU4470304.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            918..1196
                     /gene="ychH"
                     /locus_tag="ACHOQQ_RS10245"
                     /old_locus_tag="pgaptmp_002049"
     CDS             918..1196
                     /gene="ychH"
                     /locus_tag="ACHOQQ_RS10245"
                     /old_locus_tag="pgaptmp_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGU4470305.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(1251..2930)
                     /gene="dauA"
                     /locus_tag="ACHOQQ_RS10250"
                     /old_locus_tag="pgaptmp_002050"
     CDS             complement(1251..2930)
                     /gene="dauA"
                     /locus_tag="ACHOQQ_RS10250"
                     /old_locus_tag="pgaptmp_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGU4470306.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(3055..4002)
                     /gene="prs"
                     /locus_tag="ACHOQQ_RS10255"
                     /old_locus_tag="pgaptmp_002051"
     CDS             complement(3055..4002)
                     /gene="prs"
                     /locus_tag="ACHOQQ_RS10255"
                     /old_locus_tag="pgaptmp_002051"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGU4470307.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(4153..5004)
                     /gene="ispE"
                     /locus_tag="ACHOQQ_RS10260"
                     /old_locus_tag="pgaptmp_002052"
     CDS             complement(4153..5004)
                     /gene="ispE"
                     /locus_tag="ACHOQQ_RS10260"
                     /old_locus_tag="pgaptmp_002052"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGU4470308.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVAPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(5004..5627)
                     /gene="lolB"
                     /locus_tag="ACHOQQ_RS10265"
                     /old_locus_tag="pgaptmp_002053"
     CDS             complement(5004..5627)
                     /gene="lolB"
                     /locus_tag="ACHOQQ_RS10265"
                     /old_locus_tag="pgaptmp_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGU4470309.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            5841..7097
                     /gene="hemA"
                     /locus_tag="ACHOQQ_RS10270"
                     /old_locus_tag="pgaptmp_002054"
     CDS             5841..7097
                     /gene="hemA"
                     /locus_tag="ACHOQQ_RS10270"
                     /old_locus_tag="pgaptmp_002054"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGU4470310.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7139..8221
                     /gene="prfA"
                     /locus_tag="ACHOQQ_RS10275"
                     /old_locus_tag="pgaptmp_002055"
     CDS             7139..8221
                     /gene="prfA"
                     /locus_tag="ACHOQQ_RS10275"
                     /old_locus_tag="pgaptmp_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGU4470311.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            8221..9054
                     /gene="prmC"
                     /locus_tag="ACHOQQ_RS10280"
                     /old_locus_tag="pgaptmp_002056"
     CDS             8221..9054
                     /gene="prmC"
                     /locus_tag="ACHOQQ_RS10280"
                     /old_locus_tag="pgaptmp_002056"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4470312.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            9051..9443
                     /gene="sirB2"
                     /locus_tag="ACHOQQ_RS10285"
                     /old_locus_tag="pgaptmp_002057"
     CDS             9051..9443
                     /gene="sirB2"
                     /locus_tag="ACHOQQ_RS10285"
                     /old_locus_tag="pgaptmp_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGU4470313.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            9447..10256
                     /gene="sirB1"
                     /locus_tag="ACHOQQ_RS10290"
                     /old_locus_tag="pgaptmp_002058"
     CDS             9447..10256
                     /gene="sirB1"
                     /locus_tag="ACHOQQ_RS10290"
                     /old_locus_tag="pgaptmp_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGU4470314.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            10292..11146
                     /gene="kdsA"
                     /locus_tag="ACHOQQ_RS10295"
                     /old_locus_tag="pgaptmp_002059"
     CDS             10292..11146
                     /gene="kdsA"
                     /locus_tag="ACHOQQ_RS10295"
                     /old_locus_tag="pgaptmp_002059"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGU4470315.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(11295..11402)
                     /locus_tag="ACHOQQ_RS10300"
                     /old_locus_tag="pgaptmp_002060"
     CDS             complement(11295..11402)
                     /locus_tag="ACHOQQ_RS10300"
                     /old_locus_tag="pgaptmp_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4470316.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(11831..11938)
                     /locus_tag="ACHOQQ_RS10305"
                     /old_locus_tag="pgaptmp_002061"
     CDS             complement(11831..11938)
                     /locus_tag="ACHOQQ_RS10305"
                     /old_locus_tag="pgaptmp_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4470317.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(12343..13443)
                     /gene="chaA"
                     /locus_tag="ACHOQQ_RS10310"
                     /old_locus_tag="pgaptmp_002062"
     CDS             complement(12343..13443)
                     /gene="chaA"
                     /locus_tag="ACHOQQ_RS10310"
                     /old_locus_tag="pgaptmp_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGU4470318.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            13713..13943
                     /gene="chaB"
                     /locus_tag="ACHOQQ_RS10315"
                     /old_locus_tag="pgaptmp_002063"
     CDS             13713..13943
                     /gene="chaB"
                     /locus_tag="ACHOQQ_RS10315"
                     /old_locus_tag="pgaptmp_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGU4470319.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14101..14796
                     /gene="chaC"
                     /locus_tag="ACHOQQ_RS10320"
                     /old_locus_tag="pgaptmp_002064"
     CDS             14101..14796
                     /gene="chaC"
                     /locus_tag="ACHOQQ_RS10320"
                     /old_locus_tag="pgaptmp_002064"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGU4470320.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTALQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14840..15193)
                     /gene="ychN"
                     /locus_tag="ACHOQQ_RS10325"
                     /old_locus_tag="pgaptmp_002065"
     CDS             complement(14840..15193)
                     /gene="ychN"
                     /locus_tag="ACHOQQ_RS10325"
                     /old_locus_tag="pgaptmp_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGU4470321.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            15379..16773
                     /gene="ychO"
                     /locus_tag="ACHOQQ_RS10330"
                     /old_locus_tag="pgaptmp_002066"
                     /gene_synonym="iatA"
     CDS             15379..16773
                     /gene="ychO"
                     /locus_tag="ACHOQQ_RS10330"
                     /old_locus_tag="pgaptmp_002066"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGU4470322.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(16774..17424)
                     /gene="narL"
                     /locus_tag="ACHOQQ_RS10335"
                     /old_locus_tag="pgaptmp_002067"
     CDS             complement(16774..17424)
                     /gene="narL"
                     /locus_tag="ACHOQQ_RS10335"
                     /old_locus_tag="pgaptmp_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGU4470323.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(17417..19213)
                     /gene="narX"
                     /locus_tag="ACHOQQ_RS10340"
                     /old_locus_tag="pgaptmp_002068"
     CDS             complement(17417..19213)
                     /gene="narX"
                     /locus_tag="ACHOQQ_RS10340"
                     /old_locus_tag="pgaptmp_002068"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGU4470324.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
BASE COUNT         4864 a         4899 c         5037 g         4617 t
ORIGIN      
        1 gaaataactt tgcctattat acacggcact cgacaaaaat gccgcagaca acgacttatt
       61 gcgctttaaa ggcgtgcaat cggttcgttg ctttggtcaa gccatctgta aaccacattt
      121 cagtacaacg cgccgcttcg tcgatggctt catcaattaa cttctgttca ctaacaggcg
      181 gtttgcctaa cacaaaaccg acaactttat ttttatcgcc cggatgaccg attccgatgc
      241 gtaaacggtg aaagttaggg ttattaccca atttactgat gatgtctttc agtccattgt
      301 gaccaccatg gccaccgccc aacttaaatt tggcgacgcc aggaggcaga tccagttcgt
      361 cgtgggccac cagaatttcg tccggattaa tgcggaaaaa actggccatc gccgcaacgg
      421 ctttgccgct gagattcata aatgtagtcg ggactaacag gcggacatct tcgcctccaa
      481 gggtgactcg cgaagtataa ccaaagaatt tagcctcttc gcgcagcgga gcgcgcaaac
      541 gctctgccag taagtcaacg aaccaggcac cagcattatg tcgcgttgcg gcgtattcag
      601 caccggggtt cgccaggccg acaatcaatt taatcgtcac gtttttttgt cctgagtgtg
      661 tacataactg gcgcgtagtt tactggttgc ggccccgctt gacaaaaaac tgcgtatcaa
      721 atgcagataa cgtaataatt gcctgagtgg actattagaa agtcagggtg ttcaggcgtt
      781 tatttgtaaa gttttgttga aataagggtt gtaattgtga tcacgcccgc acataaccca
      841 cagggtgttg tctatacttt acacataagg aagaggggta ttccctgtta caacccagaa
      901 agttccggag gtgacatatg aaacgcaaaa acgcttcgtt actcggtaac gtgctcatgg
      961 ggttgggtct ggtggtaatg gtggtcggcg tggggtattc aatcctcaac cagttaccac
     1021 agtttaatat gccccagtat ttcgcacatg gtgcagtgct aagtattttc gtcggtgcca
     1081 ttctctggct ggcgggtgcc cgtgttggcg ggcatgaaca ggtgtgcgac cgttactggt
     1141 gggttcgcca ctatgacaaa cgttgccgcc gtagcgataa tcgccgtcat agctaacaaa
     1201 tactgcagtt tgctgaccag tcagtttgcg ctgactggtc aatctcgaac ttataaatcc
     1261 gccatcgccg ctcgacgatt cgggaagaac gcgaggcgtc ccgggatcgg ttgaatgcct
     1321 gcgcgagcca tagtgcgcag tggctggaat tccacgttgc acacgcgcag ttcacatcct
     1381 tcgggcagac gcttcacaaa acgctggaac gcatcaagac caccagcatc aagtaccgga
     1441 acggcatccc acttcagaat cacaatccgt ttgccttcaa gacgtgactc caggtccgtg
     1501 aataagcctt cagcagcagc aaaaaacagc gggccaataa cgcgcagaac caggacatcg
     1561 tctggaacat ctacgactac cggtgccagg cgagtcatac gtgcgatacg acgcataaac
     1621 agcagcgatg ccagcacgat ccccacgctg atggcaataa ccatatcaaa cagcacggtc
     1681 agcgacatgc acagcagcat gacaatgatg tcatctttcg gtgcatgacg cagcaagtcg
     1741 accactttat gcgcttcact catgttccac gccaccatca acagcagggc tgccatagcg
     1801 gaaagcggca gccaggagag cagcggtgcc agtaccagca gggcaagaat aaccagaata
     1861 gagtggatca ccgccgagat aggggaagtt gccccggcac ggacgttagc ggcagaacgc
     1921 gcgatggcag ctgtagcggt aataccacca aagaacggag cgatgatatt ccccagtccc
     1981 tgtccaacca gttcgctatt cgccttgtgt ttcgtcccgg tcataccatc cagcaccacg
     2041 gcgcagagca gagattcgat tgcgccgagc attgccattg agaatgccgc aggcagcagt
     2101 gtgcgaatag aatcccaggt taacgtgaat tctgaattag gcagatccca cggcagcacc
     2161 agttgcggca gcagttgcgg aataccgtta ccctgagaac catcggccag gacgtagtgg
     2221 aattgcgaac cgatggtagc aacatgtccg ccgagcaggt taacaatccc catcaccgcg
     2281 caaccagcca gcaaggccgg aaggtgaccg ggtaaacgaa tgcccagacg cggccagaaa
     2341 acaagaatac ctagcgtcac aatgccaatg gcagcatcac ccacattaat ggtcggcagc
     2401 gccataaata atgcgccgac tttttgtaga taatgttccg ggacatgggc catttgcaga
     2461 ccgagaaaat ctttaatctg catggtaccg atggtgatcc cgatacccga ggtgaaacct
     2521 aaggtgacgg aaaccggaat atactcaatc aggcgaccaa agcgtgccag acccatcaga
     2581 atcaaaaaga tccccgacag caaggtcgca accagcagtc ctgccagccc aaactgttgc
     2641 gaaacgggat agagaattac cacaaatgcc gcagtcggac cggaaacgct aaagcgtgac
     2701 ccacccgtca gagcaatgac aatccccgca acagctgcgg tatataaacc gtactggggt
     2761 gccacaccac taccaatagc caacgccatc gccagcggga tagcaataat cccgacggtt
     2821 atcccggcaa tcaggtcacg ggtaaaccgt gcggcagtat atttttcttt ccagcaagcg
     2881 tcgatcagag cgcggaaagg catcacatgt gaggaaaata ttttgttcac aataatgttt
     2941 catccgtgag cgcatcatct gtcaactaaa tggcaggtga aggaggcata ggtcatacaa
     3001 atggatatta cagacaaaaa aacccgccgc agcgggtctt tgagccgggt tcgattagtg
     3061 ttcgaacatg gcagagatcg attcttcgtt gctgatacga cgaatcgctt cggccagcat
     3121 acctgacagg gtcagagtac gcacgttcgg cagtgatttg atttcatcgc tcagcggaat
     3181 ggtatcgcag acaacgactt catcaattac agagttacgc aggttgttcg ccgcgttgcc
     3241 agagaagatc gggtgagtcg catacgcaaa tacacgttta gcaccacgtt ctttcagagc
     3301 ttcagcagct ttacacagcg taccgccagt gtcgatcata tcatcgacca gtacgcagtc
     3361 acgacctgca acgtcaccga tgatatgcat cacctgggaa acgttcgcac gcggacgacg
     3421 tttgtcgatg attgccatat cggtatcgtt cagcagctta gcgatagcgc gggcacgcac
     3481 aacgccgccg atgtccggag aaaccacaat tgggttatcc agattcagct gcagcatgtc
     3541 ttccagcagg atcgggctac caaatacgtt atcgaccgga acgtcgaaga aaccctgaat
     3601 ctgttcagcg tgcagatcca ctgtcagcac acggtcaaca ccgacgctgg agagaaagtc
     3661 tgcaaccact ttcgcagtga ttggtacacg agcggaacgg acgcgacggt cctggcgcgc
     3721 atagccaaag taggggataa cagcggtgat acgacctgcg gaagcacgac gcagggcatc
     3781 aaccataacg actaattcca tcaggttgtc gttagtaggg gcacaagtgg actggatgat
     3841 gaaaatatca ccaccgcgta cattttcatt aatttgtacg ctgacttcgc catcgctaaa
     3901 gcgacctaca gcggcgtcgc cgagtgaagt gtacaggcgg ttggcaatac gttgtgctag
     3961 ttccggggtg gcgttaccag caaaaagctt catatcaggc acgagaagaa cctcaggcat
     4021 gcgtccattg gtggaaagaa tctgccgaaa actgtgcggg ccaggcatga tcctttccag
     4081 gcggtgtatt aaagagcgcg atgcaacgtc tggaacaagg tgacgttgtc accgaaactc
     4141 agcttgcccg gcttaaagca tggctctgtg caatggggaa agattagcgc ctttcgccac
     4201 aaagccattg agccattccg gggcttgctc tagcacctgg cgggcttcag actctgtatc
     4261 aaattcagca aagacacagg cccctgtccc agtcaggcgc gacggggcgt attctaacag
     4321 ccaggaaagc accgcatcaa cctcgcgaaa acgttttctt gcgataacct cgcaatcatt
     4381 gctgaattca cattttagca acgtttctat tgaccttttt ggcgtattgc gcgggagttc
     4441 aggatcttta aaaatcaccg gagtcggaat acttacacca gggtgcgcca ccagatacca
     4501 cttctctggc ggagccaccg gcgttagtat ttcaccaacg ccttcggcaa acgcggcatg
     4561 cccccgaaca aagacaggaa catctgcgcc cagcgtcagc cccatttccg ccagctcatc
     4621 catgcttagc ccgcattgcc agagatgatt taatgccacc aggaccgtcg cggcattgga
     4681 tgaaccaccg ccgagaccgc cgcccatcgg caaacgcttg tcaatgctga tattcgcacc
     4741 gcttcccgtc ggaagacgcc cgctgtctgc cgcagttttc atcaataacc gcgctgcgcg
     4801 aacgatcagg ttatcttcat gttccacgcc ttcaacgggc gttaacagac gaatatcccc
     4861 atcgtcacga agctcaatgc tgatggtgtc gccgtaatca agaaactgaa acagcgtttg
     4921 cagcgtgtgg taaccatccg cacgctgacc ggtaatgtat aaaaacagat taagttttgc
     4981 cggagagggc cactgtgtcc gcattatttc actatccagt tatccatttt taacttgatg
     5041 cgttgaccac cgtcggtgag ttccatattg gctggcatcg caggttgcgt tttggtgtca
     5101 taaccaccat aaacaacctt ccagtttttg ccattctggc tgtaggtaat ttcgctcagg
     5161 cggtactggt cgtccagttt gtagtcggtt gcatcacccg gtaaacctaa aatccactgg
     5221 cgcaagctgt tgagcggaat tggcattcca gtcaatttgc caatcatctc ttcggcgtca
     5281 tcggcggtat aacgctgacc tttattgtcg actaactgca cgttacccgg ttgagcattc
     5341 agctccagtt ccgtgctgcc caatgggtta gtgagcagca gacggtagcg atcctggccg
     5401 gtttgctgcc agaaaaagcg ggcgtacact ttttgttggt cagaaatata agcgaacgcg
     5461 ccgcgagtct gatactgatt aagattgcgc acgtcttgct gatgctgacg ccattgtggc
     5521 gaatccgggc ttttgccagg acctttgggc gtggtaacgg aacaggcagt gagcacaaga
     5581 gcagccagcg gtagcaggcg gataagacga aaatcgggca ggggcatagt gatgacaagt
     5641 ccttgagata cgttgcagtt ataaccctta atgctagcgt taccgtccgc tatcgtctat
     5701 gttcaagttg tcttaattgc cagaatctaa cggctttcgg caattactcc aaaagggggc
     5761 gctctctttt attgatctta cgcatcctgt atgatgcaag cagactaacc ctatcaacgt
     5821 tggtattatt tcccgcagac atgacccttt tagcactcgg tatcaaccat aaaacggcac
     5881 ctgtatcgct gcgagaacgt gtatcgtttt cgccggataa gctcgatcag gcgcttgaca
     5941 gcctgcttgc gcagccgatg gtgcagggcg gcgtggtgct gtcgacgtgc aaccgcacgg
     6001 aactttatct tagcgttgaa gagcaggata acctgcaaga ggcgttaatc cgctggcttt
     6061 gcgattatca caatcttaat gaagaagatc tgcgtaaaag cctctactgg catcaggata
     6121 acgacgcggt tagccattta atgcgtgttg ccagcggcct ggattcactg gttcttgggg
     6181 agccgcagat cctcggtcag gttaaaaaag cgtttgccga ttcgcaaaaa ggccatatga
     6241 aggccagcga actggaacgc atgttccaga aatctttctc tgtagcgaaa cgcgttcgca
     6301 ctgaaacaga tatcggtgcc agcgctgtgt ctgtcgcttt tgcggcttgt acgctggcgc
     6361 ggcagatctt tgaatcgctc tctacggtca cagtgttgct ggtaggcgcg ggcgaaacca
     6421 tcgagctggt agcgcgtcat ctgcgcgaac ataaagtaca gaagatgatt atcgccaacc
     6481 gcactcgcga acgtgcccaa attctggcag atgaagtcgg cgcggaagtg attgccctga
     6541 gtgatatcga cgaacgtctg cgcgaagccg atatcatcat cagttccacc gccagcccgt
     6601 taccgattat cgggaaaggc atggtggagc gcgcattaaa aagccgtcgc aaccaaccaa
     6661 tgctgttggt ggatattgcc gttccgcgcg atgttgagcc ggaagttggc aaactggcga
     6721 atgcttatct ttatagcgtt gatgatctgc aaagcatcat ttcgcacaac ctggcgcagc
     6781 gtaaagccgc agcggttgag gcggaaacta ttgtcgctca ggaaaccagc gaatttatgg
     6841 cgtggctgcg agcacaaagc gccagcgaaa ccattcgcga gtatcgcagc caggcagagc
     6901 aagttcgcga tgagttaacc gccaaagcgt tagcggccct tgagcagggc ggcgacgcgc
     6961 aagccattat gcaggatctg gcatggaaac tgactaaccg cttgatccat gcgccaacga
     7021 aatcacttca acaggccgcc cgtgacgggg ataacgaacg cctgaatatt ctgcgcgaca
     7081 gcctcgggct ggagtagcag tacatcattt tcttttttta cagggtgcat ttacgcctat
     7141 gaagccttct atcgttgcca aactggaagc cctgcatgaa cgccatgaag aagttcaggc
     7201 gttgctgggt gacgcgcaaa ctatcgccga ccaggaacgt tttcgcgcat tatcacgcga
     7261 atatgcgcag ttaagtgatg tttcgcgctg ttttaccgac tggcaacagg ttcaggaaga
     7321 tatcgaaacc gcacagatga tgctcgacga tcctgaaatg cgtgagatgg cgcaggatga
     7381 actgcgcgaa gctaaagaaa aaagcgagca actggaacag caattacagg ttctgttact
     7441 gccaaaagat cctgatgacg aacgtaacgc cttcctcgaa gtccgagccg gaaccggcgg
     7501 cgacgaagcg gcgctgttcg caggcgatct gttccgtatg tacagccgtt acgccgaagc
     7561 ccgccgctgg cgggtagaaa tcatgagcgc cagcgagggt gaacatggtg gttataaaga
     7621 gatcatcgcc aaaattagcg gtgatggtgt gtatggtcgt ctgaaatttg aatccggcgg
     7681 tcatcgcgtg cagcgtgttc ctgctacgga atcgcagggt cgtattcata cttctgcttg
     7741 taccgttgcg gtaatgccag aactacctga cgcagaactg ccggacatca acccagcaga
     7801 tttacgcatt gatactttcc gctcgtcagg ggcgggtggt cagcacgtta acaccaccga
     7861 ttcggcaatt cgtattactc acttgccgac cgggattgtt gttgaatgtc aggacgaacg
     7921 ttcacaacat aaaaacaaag ctaaagcact ttctgttctc ggtgctcgca tccacgctgc
     7981 tgaaatggca aaacgccaac aggccgaagc gtctacccgt cgtaacctgc tggggagtgg
     8041 cgatcgcagc gaccgtaacc gtacttacaa cttcccgcag gggcgcgtta ccgatcaccg
     8101 catcaacctg acgctctacc gcctggatga agtgatggaa ggtaagctgg atatgctgat
     8161 tgaaccgatt atccaggaac atcaggccga ccaactggcg gcgttgtccg agcaggaata
     8221 atggaatatc aacactggtt acgtgaagca ataagccaac ttcaggcgag cgaaagcccg
     8281 cggcgtgatg ctgaaatcct gctggagcat gttaccggca gagggcgtac ttttattctc
     8341 gcctttggtg aaacgcagct gactgacgaa caatgtcagc aacttgatgc gctactgaca
     8401 cgtcgtcgcg atggtgaacc cattgctcat ttaaccgggg tgcgagaatt ctggtcgttg
     8461 ccgttatttg tttcgccagc gaccttaatt ccgcgcccgg atacggagtg tctggtggag
     8521 caggcactgg cgcggttgcc tgaacaacct tgccgtattc tcgatctcgg gacgggtacc
     8581 ggggcgattg cgcttgcgct ggctagcgag cgcccggact gcgaaattat cgctgtagat
     8641 cgtatgcctg atgctgtctc cctggcacaa cgtaatgccc agcatctggc gatcaaaaat
     8701 atccacattc tgcaaagcga ctggtttagc gcgctagccg ggcagcagtt tgcgatgatt
     8761 gtcagcaatc cgccgtatat tgacgagcag gaccctcatc ttcaacaagg cgatgtccgc
     8821 tttgagccgc tcactgcgct ggttgcggca gacagtggaa tggcagacat cgtgcatatc
     8881 atcgaacagt cgcgtaacgc gctggtatcc ggcggctttc tgcttctgga acatggctgg
     8941 cagcagggcg aagcggtgcg acaggcattt atcctcgcgg ggtatcatga cgtcgaaacc
     9001 tgccgtgact atggtgataa cgagcgcgta acgctcggcc gctattatca atgacaagtt
     9061 tttctacact gcttagtgtt catcttatta gtatcgcgct ttctgttggg ctattaacct
     9121 tacgtttctg gctacgttat cagaagcatc cacaggcatt tgctcgctgg acgcgcattg
     9181 tgccgccggt tgtcgatacg ttgttattgt taagcggcat tgcgttgatg gctaaagcgc
     9241 acatcctgcc attttccggg caggcacagt ggctgactga aaagctgttt ggagttatca
     9301 tttatatcgt tttgggtttt attgcactcg attatcgtcg tatgcacagt cagcaggcgc
     9361 gcattattgc cttcccgctg gcgttggtgg tgctgtacat catcattaaa ctcgccacca
     9421 caaaagtacc gttactgggg taagtcatga gatcgttagc tgatttcgaa tttaataaag
     9481 cgccattgtg cgaaggcatg atcctggctt gcgaagcaat ccgccgcgat tttccctcgc
     9541 aagatgttta cgacgaactg gagcgtctcg ttagtctggc gaaggaagaa atcagccagc
     9601 ttctgccttt agaagagcag ctggaaaaac tgatcgcgct gttttacggc gactggggat
     9661 ttaaagcctc acgcggcgtt tatcgtcttt ccgatgcatt atggctggac caggtgttaa
     9721 agaatcgaca gggcagtgcg gtatcattag gtgcggtttt attatgggtc gcgaatcgtc
     9781 tcgatttgcc gctgctgccg gtgattttcc ctacgcagct gatattgcgc attgaatgtc
     9841 cggatggcga aatttggctg attaatcctt ttaacggtga atcgttaagc gaacatatgc
     9901 tggacgtatg gttaaaggga aatatcagcc cgtcggcgga actgttttat gaagaccttg
     9961 atgaagctga taacattgag gtaatccgca aattgctgga tacactcaaa gcctcgttga
    10021 tggaagaaaa tcagatggag ctggcgttac gcaccagcga agctttatta caattcaacc
    10081 cggaagatcc ctatgaaatt cgcgatcgcg ggttgattta tgcgcaactg gattgcgaac
    10141 acgttgcgtt gaacgattta agttatttcg ttgaacagtg tccggaagac ccgatcagcg
    10201 aaatgatccg tgcgcaaata aataacatcg cgcataaaca tattgtgctg cattaattaa
    10261 tcgacatttt actcaaggtt aaggcgatcc tatgaaacaa aaagtggtta gcattggcga
    10321 catcaacgta gcaaatgacc tgccgttcgt actgtttggc ggtatgaacg tgctggaatc
    10381 tcgcgatctg gcgatgcgca tttgcgagca ctacgtaacc gtgacccaga aactgggtat
    10441 cccttacgtg ttcaaagcct cttttgacaa agccaaccgc tcctccatcc actcttatcg
    10501 tggaccgggc ctggaagaag ggatgaaaat cttccaggag ttgaagcaga cttttggcgt
    10561 gaaaattatc accgacgttc acgaaccaag tcaggcacag cccgttgctg atgtcgtgga
    10621 tgtgattcag ttgccggcgt ttcttgctcg ccagactgac ctggttgaag ccatggcgaa
    10681 aaccggtgcg gtaattaacg tcaagaaacc acagtttgtc agcccgggac agatgggtaa
    10741 tatcgttgat aaattcaaag aaggcggcaa cgaaaaagtg attctttgcg atcgtggtgc
    10801 taacttcggc tatgacaacc tggttgtcga tatgctgggc ttcagcatta tgaagaaagt
    10861 gtctggtaac tcgccggtga ttttcgacgt gacccacgca ttgcaatgcc gcgatccttt
    10921 tggcgcagct tccggcggtc gtcgtgctca ggtggctgag ctggcacgag ccggtatggc
    10981 ggtaggtctg gcggggctgt ttattgaagc gcatccggat ccggaacatg cgaaatgtga
    11041 tggtccatcc gcgctgccgc tggctaaact ggaaccgttc ctcaagcaga tgaaagcgat
    11101 tgatgatctg gtgaaaggtt tcgaagaact ggataccagc aagtaatctt ttttgcttga
    11161 aaaataaagt cttagcgttc tgcgttaaga cttttttcat gggtgccgga tacaaaaaag
    11221 gccgcaggct gttatccctg cggccggttt cgggcgcata ttgccatcac ggcagcctga
    11281 cgcccgtttt cgccttactt ccggttacgc caccagccga caatcgctgc ggtaataatt
    11341 cccgccagga tcggtgctgc caggtcgtgc cagaaaatca tggcaaactg cgcgagcgtc
    11401 atatagccgc cttgttgtaa tgacaacatt ttgcggctat tcttgcattg ttgatggttc
    11461 aagattagcc cccgttctgt tgtcaggttt tacctctcaa cgtgcggggg ttttctcttt
    11521 ccagcaacca atgccaccag ggataaagcc cccgcaacat tgcgcctcac cggataacgc
    11581 cggcttggtg tggatactac gactcaattc atcttcactt catccctgaa atgtttgcaa
    11641 tgaagagtgc attccggttt ttcaacagct gttacagtcg ttttatgagt gctctggatg
    11701 atgcttccag ctcgggttgc caatatttac ttgtggaagt gataaagaca aaaatggccg
    11761 caggctgtta cccctgcggc cggtttcggg cgcatattgc catcacggca gcctgacgcc
    11821 cgttttcacc ttacttccgg ttacgccacc agccaacaat cgctgcggta ataattcccg
    11881 ccaggatcgg tgccgccagg tcgtgccaga aagtcatggc aaactgcgcg agcgtcatat
    11941 aaccgccttg ttgtaatgac aacattttgc ggctattctt gaagtgtctg gtttcaagat
    12001 tagcccccgt tctgttgtca ggttttacct ctcaacgtgc gggggttttc tctttccagc
    12061 aaccaatgcc accagggata aagcccccgc aacattgcgc ctcaccggat aacgccggct
    12121 tggtgtggat actacgtctc aattcatctt cacttcatcc ctgaaatgtt tgcaatgaag
    12181 agtacattcc ggcttttcaa cagctgttgc agtggtttcg tgcaggttgt ggtacaggct
    12241 cgcaattctg attaacgacc tgtccaggta gtatgaagaa atacaataag caggaattta
    12301 tattatctcc cccgatttcc gtcatcggag gagatataac tgtcaggcaa atatcgtcat
    12361 caaataggcg gcaaacagtg ccagatgcgc tgcgccattg agcacgttag tacgtccggt
    12421 ggagaaggag atatggcaca gcactaaaga ggccaccatc accaccattt ctggcgcacc
    12481 aagtgcaaac tgcaattcgt tacccgtcat aaatgcaatt agcgtgacga caggtacggt
    12541 aagcgaaatt gttgctaaca ctgaaccaaa gaacagattc atcgcgcgct ggacctggtt
    12601 gttcaacact gcttttaatg cacctaaacc ttccggcgac agaatcaaca gtgccaccag
    12661 gaagccagta aaggcgacag gggcattcat gctgtcgagc aaagtctcca gcgagctggc
    12721 gttcattttg gtcaccgcaa taacggcaat cagatggata atcaaccaga tagcatgcca
    12781 caggctgcta tgggcagacg gtttaccgtg gtgcgggtcg tcgtcatcac tgtcatcttc
    12841 gtgctcgtag acaaacaaac tttgatgcgt tttggtctgg atcagcaaaa atacgccata
    12901 cattgccgca gaaattaatg ctaccagtaa cgcctgaccg gttgaaaaat tcgccgcagg
    12961 cagagccatt ggaaatacca gtacgattat cgccaggggg aacagcgcaa ttaaatactg
    13021 cttgatacca aacagattca tatattgggt ggcaaactta cgaccgccca acaataatga
    13081 aaagccaacc agcccaccgg taacaatcat aatgattgaa tagagcgtat cacgcattag
    13141 cgttggcgcg gcgtcgccgg ttgccattaa agctgaaatc aaactgactt caagaataac
    13201 cactgaaagg ctaagaataa gcgaaccgta aggttctccc aggcgatggg ctaatacgtc
    13261 cgcatgacgg acaacactaa aggcgctact taaaatacca ataagcgcaa gaagattgat
    13321 ggcaatgacc actggtagtg tctggctgct tccccacagg aacagcacta ccagcgccag
    13381 aaccgggaaa ataagcgaag tctccttgtg gcgggttttt accgcctctt gagcatttga
    13441 cattatggtt atccctttgc agatgaattt gtcgaaaatg taaaaaatag gtaggaaaaa
    13501 taacagaaag tgtctggata tcggtaacat tttacgaaat tttaccccgc tgtcgatttt
    13561 ttactcattg gggcataaaa ataagtaata cgtttagaca atgtttgttt tagcgtttct
    13621 taagaacagt ctgacatgaa aattcttatg ttttggcaag agtagatatt gttgaccaca
    13681 cttaatgttc aactttgtaa aaggagtcaa cgatgccgta taaaacgaaa agcgacctgc
    13741 cggaaagcgt aaagcacgtt ctaccgtctc atgcccagga tatctataaa gaagcgttca
    13801 acagcgcatg ggatcaatat aaagataaag aagatcggcg tgatgacgcc agtcgcgaag
    13861 aaaccgcgca taaagtggcc tgggctgctg tgaagcatga atatgccaaa ggggatgatg
    13921 ataaatggca taaaaaatcg taaaaccggt agcttagtta aagctattcg tgcggtgttg
    13981 ccttgcaagt ggtccgcgga ttgcatattg tcccgttagt ggtttcaaaa tgagcagtaa
    14041 aaatgtccgg aagacaccaa aaagttgtcg cagggaagca tgcagtggcg gaggtgtaag
    14101 gtgataacgc gtgatttctt gatgaatgcc gattgtaaaa cggcatttgg tgccattgaa
    14161 gaatcactct tatggtcagc agaacaacgg gcggcttcgc tggcggcgac gctggcttgt
    14221 cgacctgatg agggaccagt gtggatcttc ggttatggat cgttgatgtg gaatccggca
    14281 ctggagttta ccgagtcgtg caccggtaca ctggttggat ggcatcgtgc gttttgcctg
    14341 cgtctgaccg ccgggcgcgg gactgcgctc cagccgggac ggatgcttgc actgaaagag
    14401 ggcggacgca ccacaggcgt cgcctatcga ctgccagaag agacgctgga gcaggaacta
    14461 accctgttgt ggaagcgcga gatgattacc ggctgttatc tgccaacctg gtgtcagctt
    14521 gatcttgatg atggacgcac agtaaacgcc attgtgttta ttatggaccc gcgacatcca
    14581 gaatatgaat ctgacactcg cgctcaggtc atcgcgccgt tgattgcggc ggcgagcggt
    14641 ccgctgggaa ccaacgcaca atacctgttt tcactggaac aggagcttat caaactggga
    14701 atgcaggatg atgggctgaa tgatttgctg gtatcggtaa aaaaactgct ggcggagaat
    14761 tatccggatg gtgtgttgcg gccgggattc gcctgagtaa acttccctca tcgtggggcg
    14821 tcagacgccc cctcaaacat taaaatgtga gcactttatc ggctgacagc gtccattgcg
    14881 ccagttccac aagagtaccg atttccaccc catcaatcag aggaagtgta ctaatcccgc
    14941 gcccgtcggt acaggttttg cacaatttca ccggtacatt ctgagcggta aggatctcca
    15001 gcatttgctg aatgttgtag ccttcccctg gtttttgccc gcgcaacccg gctgtgaccg
    15061 catcagacat gaggaacaga cgcagatcca gattgctctc ctgctctcgt aacgcaatgg
    15121 ccagccgcaa gctgttaaac aaggattcgc tcccgtaagg tgcgccattg gcaacgatca
    15181 caattttttg cattatttac tcctgtattc agggaattag acactcatct tctatcttac
    15241 tgcttctgca gcgtctgacc aatcggtcac atttttaagg attttcctga aagcgcgaga
    15301 aaatacgaca aaagttgcca gtaatcgtta ttctttaagg ctatggtttt tcatttttta
    15361 ccggaagtta ccgacgtttt gagccgtttc gttcctcgca ttattccgtt ttatttactc
    15421 ttgctggcgg caggcggtac agctaacgca caatctacct tcgagcaaaa agcggcaaat
    15481 ccctttgata ataacaatga tggtctgccg gatttaggca tggcacctga aaatcatgat
    15541 ggggaaaaac actttgctga aattgtgaaa gatttcggcg aaaccagtat gaatgataac
    15601 gggctggata ctggcgagca ggcaaaagct ttcgcattgg gaaaagtccg cgacgcgctt
    15661 agtcaacagg ttaatcagca cgtagagtcc tggctatcac cgtggggaaa tgccagtgtt
    15721 gacgtcaaag tggataacga aggacatttc accggcagtc gtggaagctg gtttgtgccg
    15781 ttacaagata atgatcgtta tctcacctgg agccagcttg gtcttactca gcaggatgat
    15841 gggttggtga gcaatgtggg cgttgggcaa cgctgggcgc gcggcaactg gctggtgggt
    15901 tataacactt tttatgacaa cttgctggac gaaaatcttc agcgagcggg ctttggtgcc
    15961 gaagcgtggg gcgaatattt gcgactatcg gcaaactttt atcagccatt tgctgcatgg
    16021 catgaacaga cagccacgca ggaacaacgg atggcgcgcg ggtacgacct gacagcccgg
    16081 atgcgcatgc cgttctatca acacctcaat accagtgtca gcgtagaaca gtattttggt
    16141 gatcgtgttg atttgtttaa ctctggtacg ggttatcaca atcccgtcgc gttgagtctg
    16201 ggattaaatt acacccctgt gccattagtc actgtgacgg cccagcataa acagggtgaa
    16261 agtggcgaga atcaaaataa cctcgggctg aatcttaatt accgctttgg tgtaccgctc
    16321 aaaaaacaac tttctgcggg cgaggttgcc gaaagtcagt cgttacgtgg tagtcgctat
    16381 gataatccgc agcgaaataa tctaccgact cttgagtacc gacagcgaaa aacgttaacg
    16441 gtgtttctgg cgacaccgcc gtgggatcta aaacctggcg aaacagtgcc gctgaaatta
    16501 caaatccgca gtcgttacgg tattcggcaa ctgatttggc agggcgatac gcagatatta
    16561 agtttgacgc cgggcgcaca agccaacagc gcggagggct ggacgctgat catgcctgac
    16621 tggcagaacg gggaaggggc gagcaatcac tggcgattgt cggtggtggt ggaagataac
    16681 caggggcagc gtgtctcctc caatgagatc acgctaacgc ttgtcgaacc gttcgacgca
    16741 ttgtcaaacg acgaactgcg ctgggaaccg taatcagaaa atgcgctcct gatgcaccca
    16801 taccgctgct tccacgcgag acttgagctt cattttcttc agcatgtgct tgacgtgcac
    16861 ttttactgtg ctttcggtga tgtccaggcg gcgggcaatc atcttgttcg gcaaaccctg
    16921 ggcaatcagc ttgagaatat cgcgctcgcg gggggttaac tggttaacat cgcgctcagt
    16981 cgtggcacgg ttagcgcgca agctggcggc cagaacaggc gttaatgctt cgcttaatac
    17041 catttcgcca gcagctgcct gatgcaatgc tttcagcaga tcttccggtt ccatatcttt
    17101 taacagatag ccatccgcgc cgcgtttcag tgcggtgacc acatcttctt catggttaga
    17161 gacactgaat accacaatgc gccctgagag ggacttttcg cgcagtttat ccagcgtttc
    17221 cagaccgttc atgccgggca tattgagatc taacaggatc agatcgggat caagagattc
    17281 cgccagttca ataccctgtt cgccattact cgcttcgcca accacggtga tatctggtgc
    17341 catactgata agctgtttta cgccagttcg cagcatcggg tgatcgtcaa tcagcaggat
    17401 agtagccggt tcctgattac tcatgggtat ctccttggac gtctgtgaaa gttttttcgg
    17461 gaataaaggt gaccaccact tcggtgccac ctgattcacg acggcggacg cggcaatcgc
    17521 ctcgtaaact ttgcgcacgg tcgcgcatta ttatcatgcc gtagtgattg ctgcggatgg
    17581 cattttcagg cacgccgcag ccgttatcct ggacggtcag tttgacctga ttatcgtttt
    17641 gcgccaccgt caccacgacc tcactcgctt gcgaatgttt gagggcgtta cttaatgcct
    17701 cacgggcaat ttgcaacaag tggattgcct gatgcgaagg caccagacga ggcggcaatt
    17761 gataatccag cttcaccggg aagccaaatt tggcgctgta ctcttcgcaa ctcgcctcca
    17821 gcgccggacg taatccaggc tcggtgagct gcaagcggaa tgtggtgagc aattcacgca
    17881 actgcgccca ggatgcattc agttcgttac ggatctgact taacagttcg cggctgcttt
    17941 ctggcagcgc atcgccctgc atctgtaaac aactcacctg catcttcatg caagagagag
    18001 attgggcaat agaatcatgc agttcgcgcg caatggtggc acgctcttcc atcacgatca
    18061 actgttgctg acgttcctga tgccgatcca gcgccagcgt ggcggtgagt tgttcaacca
    18121 gggtatccac cagttgttgt tgatcatggc taagatgacg cccctgcggc agggtcgcca
    18181 gcaaaatacc gtactgcgta tgagagtcag ccagccgcca cttcagggtc gtgccgcgat
    18241 caccaacggg taatacgccg cgcgggcaga gctggcagcc tttatcatca caagtcatat
    18301 ctggctggca ggtaaactcc tgatgattct cttcatcatc agtgtcatac acccgcagtt
    18361 cgatatcacg tagcagggtt aaattctgta agccgttgag tacaggtgac aggcgttcac
    18421 acagcggggc gcgggaatgc aaacggcggt tagcctgcca taaaaaagag aggatctgat
    18481 ttttatgctc cagcccggcg gttttctcct gaacccgctg ctcaagtacg gcataacttt
    18541 cggccagttc tgcagacata ttgttcaacg cagttccaag catcgccatt tcgttgcgcc
    18601 cgctgatgtt tgcgcgttgg gtaaaatcgc gatggctgac ggcactcgcc attgccagca
    18661 gttgccgcca cggttgtagc agtcgcgccc gcaaccagat aatagtgaac accagtaaaa
    18721 gtgccataaa taccgccatt acccgatgga ccagtaccac tgtctcaata cgcatttccg
    18781 tggtgcggtc aaaaccagat accagctgat caagcccggc aacaaactgg ctgacatccg
    18841 ctgataccgt ttctcggttt tgcgcccgca tcagcgcagg gatcagttca ttacgccagt
    18901 aatcttgtaa accctgtaat tgcgccagtt gtccgtctcg ttctgctgct cgagtcaact
    18961 cggcgctaaa tgccgtttgt tccatctctt taattaaggg cttgtctttc tcgcttaatg
    19021 gcactgctgc caacagacgg taactttgca tgcgcagcga tcccgctttg ttgatcgcat
    19081 gggcgctgcc ctgaacgcct tgcaccagcc agccagaaac cgccatccct gccagtccaa
    19141 tagcagtaga aagcaacaca ataagcgcaa cctgattaac cagggtgagc ggagagagac
    19201 aacgtttaag catgtaaacc tcttccttca ggctttaaat gagcaataac cttaatgaat
    19261 gtgacgatac attctggaat ggcagtattc tcggctattg gctgaagtat acccataccc
    19321 ggaaagagtt actccttatt tgccgtgtgg ttagtcgctt tacatcggta agggtaggga
    19381 ttctacagca ccgtgaaaaa tctcataatt tttatga
//
LOCUS       DAZKFU020000074        19253 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZKFU020000074 DAZKFU020000000
VERSION     DAZKFU020000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.074"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(55..315)
                     /gene="yfhL"
                     /locus_tag="ACHOQQ_RS10345"
                     /old_locus_tag="pgaptmp_002069"
     CDS             complement(55..315)
                     /gene="yfhL"
                     /locus_tag="ACHOQQ_RS10345"
                     /old_locus_tag="pgaptmp_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGU4470325.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            530..1927
                     /locus_tag="ACHOQQ_RS10350"
                     /old_locus_tag="pgaptmp_002070"
     CDS             530..1927
                     /locus_tag="ACHOQQ_RS10350"
                     /old_locus_tag="pgaptmp_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4470326.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            complement(1924..2124)
                     /locus_tag="ACHOQQ_RS10355"
                     /old_locus_tag="pgaptmp_002071"
     CDS             complement(1924..2124)
                     /locus_tag="ACHOQQ_RS10355"
                     /old_locus_tag="pgaptmp_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4470327.1"
                     /translation="MSEKPDDLLTPDEVCQKLGITQKTLCEWNIKHRHRAILAPIRFS
                     AKVVRYERRNVDAFIQKCRSQY"
     gene            complement(2121..3515)
                     /locus_tag="ACHOQQ_RS10360"
                     /old_locus_tag="pgaptmp_002072"
     CDS             complement(2121..3515)
                     /locus_tag="ACHOQQ_RS10360"
                     /old_locus_tag="pgaptmp_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HGU4470328.1"
                     /translation="MGQIFTPRPYQDLIINHEIGISRCNIWAGMGMGKTVATLTTLED
                     LFMAGSETQPALVLAPLRVAASTWPDEALKWGHLRNIEVQPIVGNAKARSAALANSNA
                     SVFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVQ
                     RWINLTGTPAPNGLVDLWGQLWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSQAEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            complement(3560..4339)
                     /locus_tag="ACHOQQ_RS10365"
                     /old_locus_tag="pgaptmp_002073"
     CDS             complement(3560..4339)
                     /locus_tag="ACHOQQ_RS10365"
                     /old_locus_tag="pgaptmp_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4470329.1"
                     /translation="MSERTLTLAGTGKQPMMSSREIAEVVNKNHADVCRDIRAMLAKL
                     YGGDERDYIRKADLLYLTNQCVNCLQYNTDNPNAWEYQLDYEHSECLVTGYDAVRRMA
                     MIKHWQKLKKEVEPPQLAITHPAPVIQVDSRIVQLARVIAEATASATMKAMSEVIALP
                     TYQAESAAPANLSHVEGEYVPVSKAAWKTGLSDSTCRKLIGFARIPVRSDTGVRGLLV
                     NLPAIEQAAQKLLNESTPPKGNRKRWTHPQFGNFTYYTDLI"
     gene            complement(4400..4576)
                     /locus_tag="ACHOQQ_RS10370"
                     /old_locus_tag="pgaptmp_002074"
     CDS             complement(4400..4576)
                     /locus_tag="ACHOQQ_RS10370"
                     /old_locus_tag="pgaptmp_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470330.1"
                     /translation="MAIQITQLHQADIFGYIADMLETARLLSSLEKGEQLAFELIYFA
                     QQAAREAANKPWDE"
     gene            4689..5018
                     /locus_tag="ACHOQQ_RS10375"
                     /old_locus_tag="pgaptmp_002075"
     CDS             4689..5018
                     /locus_tag="ACHOQQ_RS10375"
                     /old_locus_tag="pgaptmp_002075"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU4470331.1"
                     /translation="MSKRDDPQLRVRIPELLKEALEKKARANKRTLTSEIVSRLESTI
                     LQDKLLHTSRGFEEAADEILILRDLLEKLKTTYHREYQVEWVYENKYELIEVMEKLKK
                     LLNPENE"
     gene            complement(4987..5163)
                     /locus_tag="ACHOQQ_RS10380"
                     /old_locus_tag="pgaptmp_002076"
     CDS             complement(4987..5163)
                     /locus_tag="ACHOQQ_RS10380"
                     /old_locus_tag="pgaptmp_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU4470332.1"
                     /translation="MSKRDDPQLRVRIPQDLKDTLEKISRENDRTLTAEITRRLRKSL
                     EDESTIHSQDLATS"
     gene            complement(5160..5429)
                     /locus_tag="ACHOQQ_RS10385"
                     /old_locus_tag="pgaptmp_002077"
     CDS             complement(5160..5429)
                     /locus_tag="ACHOQQ_RS10385"
                     /old_locus_tag="pgaptmp_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HGU4470333.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGRRSVPDRIVLLPG
                     GRIVFVECKAPGKPPRPDQLREHERLRKLGFTVVVLDSKNLEGIL"
     gene            complement(5430..5669)
                     /locus_tag="ACHOQQ_RS10390"
                     /old_locus_tag="pgaptmp_002078"
     CDS             complement(5430..5669)
                     /locus_tag="ACHOQQ_RS10390"
                     /old_locus_tag="pgaptmp_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470334.1"
                     /translation="MRNTAKLQLGFSPLSKKIMLAKMRDVDGGRLRVGNDPGRDVTNE
                     AAQLVWQLVMAEGGEISWGLDDGSQMVLRAEKQVK"
     gene            complement(5669..5884)
                     /locus_tag="ACHOQQ_RS10395"
                     /old_locus_tag="pgaptmp_002079"
     CDS             complement(5669..5884)
                     /locus_tag="ACHOQQ_RS10395"
                     /old_locus_tag="pgaptmp_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGU4470335.1"
                     /translation="MSFKYRDNPLYYRAAREALRLEQSGEYDRAAKVWAKANRQSRNE
                     LNQDWSERRSDFCLMQNMREKRKAVEE"
     gene            complement(5890..6093)
                     /locus_tag="ACHOQQ_RS10400"
                     /old_locus_tag="pgaptmp_002080"
     CDS             complement(5890..6093)
                     /locus_tag="ACHOQQ_RS10400"
                     /old_locus_tag="pgaptmp_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470336.1"
                     /translation="MTVIKTHTGTVITRTGEKQVQLHQNATTWVAGPKEYYYKDTGRR
                     GGAVGVRTRLVLSSIRPIEAKQE"
     gene            complement(6099..8162)
                     /locus_tag="ACHOQQ_RS10405"
                     /old_locus_tag="pgaptmp_002081"
     CDS             complement(6099..8162)
                     /locus_tag="ACHOQQ_RS10405"
                     /old_locus_tag="pgaptmp_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HGU4470337.1"
                     /translation="MSNILWGDLETFCEIPINNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIADPETLLYFHNSHFDRTVLRYAIPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDMQLARAAIEAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPHLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDIAPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYNESVKRKSTFGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEQGNITYMGVNSYSRKWQRLKTYGGKLVENVTQAAARDVLAGN
                     MPLIEDAGYSIVLTVHDEVITESPDTEDFNDKELSALLSNNPEWAPDIPLNAGGFEAY
                     HYRKD"
     gene            8189..8788
                     /locus_tag="ACHOQQ_RS10410"
                     /old_locus_tag="pgaptmp_002082"
     CDS             8189..8788
                     /locus_tag="ACHOQQ_RS10410"
                     /old_locus_tag="pgaptmp_002082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470338.1"
                     /translation="MDTKDIISLVGVIISAITAPVGVYKLFKDASWFLPKTTKFSHIL
                     KNYPEHIEPSDVEFMKAEIKREVKKSILGISSQKLRTLVIYVRTYSELKMPFWQWGYL
                     APHIQCKYDRFFIRYKGKYKRCRWCSKVVSIFYLADGLIFFCWSLNYGAVFIGMGAVL
                     MLLCIWMAFMFWFLFPGRGVIKKYNSQLLKIDASKYQAK"
     gene            complement(8903..9667)
                     /locus_tag="ACHOQQ_RS10415"
                     /old_locus_tag="pgaptmp_002083"
     CDS             complement(8903..9667)
                     /locus_tag="ACHOQQ_RS10415"
                     /old_locus_tag="pgaptmp_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HGU4470339.1"
                     /translation="MSTQIVFKSHVLETIEHNGKTWFTAATLATALEYSDARSVTNIY
                     TRNTDEFSLGMSEVIKMVTSGNLQASRRIFSLRGAHLVAMFATTPVAKEFRRWVLDLI
                     EKETAVPQSSTILAPHRECLPKMVYHHRSKYNPYRAYAWDGEKSVYVGCYPTVGEAVA
                     AQERFYRNGSTKRIQKAQPAVSDAEKEMFINNLRAVCHNFRRINEIWRAQLMPALEKM
                     DSKLVYQLHDRFSDSMCILPTIEDRIGRYIPPTLPR"
     gene            complement(10049..10597)
                     /locus_tag="ACHOQQ_RS10420"
                     /old_locus_tag="pgaptmp_002084"
     CDS             complement(10049..10597)
                     /locus_tag="ACHOQQ_RS10420"
                     /old_locus_tag="pgaptmp_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HGU4470340.1"
                     /translation="MKIKLNNVRLAFPALFEAKTVNGEGDPRFSAVFLISPKHPQLEE
                     IRKAMKQVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(10613..11914)
                     /locus_tag="ACHOQQ_RS10425"
                     /old_locus_tag="pgaptmp_002085"
     CDS             complement(10613..11914)
                     /locus_tag="ACHOQQ_RS10425"
                     /old_locus_tag="pgaptmp_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HGU4470341.1"
                     /translation="MPDVHARLSPSSAHRWLRCAGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAQNNELLVEQRVDFSHITGVEESFGTADGIIIAGNELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGKRAQEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGNPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDLKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLAADLI"
     gene            complement(11907..12425)
                     /locus_tag="ACHOQQ_RS10430"
                     /old_locus_tag="pgaptmp_002086"
     CDS             complement(11907..12425)
                     /locus_tag="ACHOQQ_RS10430"
                     /old_locus_tag="pgaptmp_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HGU4470342.1"
                     /translation="MPEAWKDISGFEGIYQISDAGNLRSLDRTVLNKGSGCTYPIKGK
                     SLKPRYDADGYLITDLWKAGKKVTVKIHRLVAAAFIPGSAPEVDHINGRRDDNRAVNL
                     RWASLSQNRANSHTRKNKSGVVGVRFSEGKKNPWQAYGRLNGKFKSLGHFPTKQLVAA
                     ARRKHIQEVMNA"
     gene            complement(12428..13330)
                     /locus_tag="ACHOQQ_RS10435"
                     /old_locus_tag="pgaptmp_002087"
     CDS             complement(12428..13330)
                     /locus_tag="ACHOQQ_RS10435"
                     /old_locus_tag="pgaptmp_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470343.1"
                     /translation="MSLETSLELNNQLLEQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATEAENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            complement(13327..13476)
                     /locus_tag="ACHOQQ_RS10440"
                     /old_locus_tag="pgaptmp_002088"
     CDS             complement(13327..13476)
                     /locus_tag="ACHOQQ_RS10440"
                     /old_locus_tag="pgaptmp_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470344.1"
                     /translation="MKPEHLYRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            complement(13578..13871)
                     /locus_tag="ACHOQQ_RS10445"
                     /old_locus_tag="pgaptmp_002089"
     CDS             complement(13578..13871)
                     /locus_tag="ACHOQQ_RS10445"
                     /old_locus_tag="pgaptmp_002089"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGU4470345.1"
                     /translation="MAGKPAGKVKADGYVTICLDGKNQYVHRLVWEYHFGDIPAGMEI
                     DHINHNRADNRIENLRLVTRAENAKNLAQRSPTKRNGGQLERIKKALAGTNHR"
     gene            complement(14059..14265)
                     /locus_tag="ACHOQQ_RS10450"
                     /old_locus_tag="pgaptmp_002090"
     CDS             complement(14059..14265)
                     /locus_tag="ACHOQQ_RS10450"
                     /old_locus_tag="pgaptmp_002090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470346.1"
                     /translation="MNTYELTTKYAPAFEVVENGPDATDYVVATVKGENPARLRAAAY
                     QERNGYLGYGYTIRPLTNSSESSM"
     gene            complement(14650..15279)
                     /locus_tag="ACHOQQ_RS10455"
                     /old_locus_tag="pgaptmp_002091"
     CDS             complement(14650..15279)
                     /locus_tag="ACHOQQ_RS10455"
                     /old_locus_tag="pgaptmp_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4470347.1"
                     /translation="MNLANRAKKRRTELNLTQVEVAKRAGISQQSIEAIENGKTLKPR
                     NLLALASALECDPKWLLLGGDVVTDFNYGARRVPVLSYVQAGAFTSAELLREEGDFEY
                     ILTTAELSESSFALRIRGDSMEPEFKEGDIVIIDTDVYPTPGEFVAACNGSHEATFKK
                     YRPVGIGAKGEEDFELVPLNSDYPICRSWEKPIKIIGTMVEHRIFRRKR"
     gene            15387..15611
                     /locus_tag="ACHOQQ_RS10460"
                     /old_locus_tag="pgaptmp_002092"
     CDS             15387..15611
                     /locus_tag="ACHOQQ_RS10460"
                     /old_locus_tag="pgaptmp_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4470348.1"
                     /translation="MTSSTLASRIKERRKALGITQTSLAESVGMRQQSVQYLESGRAT
                     RTGFILELAKVLKCDPDWLLNGENSEQQKV"
     gene            15615..17834
                     /locus_tag="ACHOQQ_RS10465"
                     /old_locus_tag="pgaptmp_002093"
     CDS             15615..17834
                     /locus_tag="ACHOQQ_RS10465"
                     /old_locus_tag="pgaptmp_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HGU4470349.1"
                     /translation="MPDNKPWGATPDEWFHFDLVLGMTDRLLPVVSNPCAELSPNSKL
                     SHNFGKTPSRYNGQRKVVGIPDWANYVVTENDFARWSNEPDYGICVRTGDGVVALDCD
                     INDAGMQEIVRNIILSCLGELPPRRWRADSHKCLYLIAVDGDYRKRGHRLEGENKQIE
                     LLAKGQQFVACGTHPAGERIQWDCGLPGEPLKITSEQLESLWQRLADNLPVKDSYTAG
                     AGRQRDLSCVDPSATDDVADWLDANGWTLSVSSDGSRNLKPFRDESEYSNGCSETSIK
                     YFPKGTGGFELGHFKSMHNTDAGLTDADWLDAYGYRVADFEDLTVAEDGDKPEYLPWP
                     KFSRDKNDRIEGTVTNVLLALRRPDLCGVQIRLDEFRDEIILITPRGKNLRLRDEFYT
                     MLHSRLECLGFKKFEEAAIRRAVRLVAYENRFDSLKDWIANLPAWDGVPRIDTFFCRH
                     WGIEANAYTHAVGRYWWTLLAGRALVPGIKGDMAVILVSQQGKNKSEGIRAMAPTPEH
                     YMELDFGKKGEERIREMRGHNVIELGEMRGMDKRGIGDVRVTISTRADRNRGLFREHY
                     VTLPRRCGFIGTSNSDTPLTDTEGNRRWLPMTIPDETDGKFIAQGIADEKIQLWAEAV
                     AVFKRDGIAWENAETLAKSILGDYEVKDEVWMSCVAEWLELPQDLYDMAADGTKKNCE
                     RVPLTSKDVLVEAIGFKAPQVKRSDEMKIAAIMKKLGYKNKQLQSLPGKPRSWEKCK"
     gene            18141..18422
                     /locus_tag="ACHOQQ_RS10470"
                     /old_locus_tag="pgaptmp_002094"
     CDS             18141..18422
                     /locus_tag="ACHOQQ_RS10470"
                     /old_locus_tag="pgaptmp_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470350.1"
                     /translation="MNTTCARLPSADTLAEQAAKTASGWMSDAVIAIDELFGKGYAKQ
                     HPELVAAFMQTAGLDEAAMHLRGLVLANQRIDDTLEVLSEHIMLLNEPQ"
     gene            18503..18913
                     /locus_tag="ACHOQQ_RS10475"
                     /old_locus_tag="pgaptmp_002095"
     CDS             18503..18913
                     /locus_tag="ACHOQQ_RS10475"
                     /old_locus_tag="pgaptmp_002095"
                     /inference="COORDINATES: protein motif:HMM:NF018262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4470351.1"
                     /translation="MQLVLERWGRWAASEEYCSMVDWPSMSVTSRDVTDSGKPGCSDE
                     DGAVIDTCIAHMSMVCPYDDLLILGLRFIGGLSTRAIAEAIDRSHLSVRTSLKASEAF
                     LEGALTLQGVRLDMDPEVVLPERVVCAQKNMLWL"
     gene            18993..19094
                     /locus_tag="ACHOQQ_RS10480"
                     /old_locus_tag="pgaptmp_002096"
                     /pseudo
     CDS             18993..19094
                     /locus_tag="ACHOQQ_RS10480"
                     /old_locus_tag="pgaptmp_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            19083..19159
                     /locus_tag="ACHOQQ_RS10485"
                     /old_locus_tag="pgaptmp_002097"
     tRNA            19083..19159
                     /locus_tag="ACHOQQ_RS10485"
                     /old_locus_tag="pgaptmp_002097"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19117..19119,aa:Arg,seq:tct)
BASE COUNT         4557 a         4619 c         4878 g         5199 t
ORIGIN      
        1 tagcgttaag gtttccctat aaaataatta atactctact tcaggagcag aatattaaat
       61 tttatccgcg tggtgcatca gcacaaattt atcccacaac tgttcttctg tctcgacatg
      121 ttgcggatct ttcacaatag tattggggat cgggcacacc ttctggcagg ttggtgtctc
      181 gtagtgccct acgcattcgg tacacttatc gctgttaatc tcatagatat gatctcccat
      241 tgaaatcgcc tcattcgggc attcgggttc acacatatca caattaatac agcgtttagt
      301 aattagtaaa gacatttcaa tggattaccg ttaaatcatt ttaaaatcag taagttgtat
      361 cgagtttgta tgctttactg tcattaactt actgtatgtt gatccagtgt atttaacctt
      421 gataaactca gtccagcaac acaaaaccgc aacacattgc attttgtccc gtagaaaaga
      481 cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa ggattgagaa tgccgcgcac
      541 tgtaacacat aatccggata gccccaataa tgacgatgtt ttagccgcat ctgaaaaatg
      601 ggacgcctgt aaacccccct ataccagcgc acacatgaaa atctgtgttg ctgccgctaa
      661 aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa tacgaaaaag agaactatct
      721 ccgtatcgat ttcagcaaag ccggtaaggt tacattttat gccgagtttc caaaaaagat
      781 gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc gctatccagc tggcgcgcga
      841 aaaagcgcta ggtatggctg acggtggcct gcgggcagag tccgtacatg cagcgctgga
      901 aatgtaccgg gatgacctca aagccaaagt cgcccggcag aagctgagcc cggacagttt
      961 cacaacctac ggggtgcgta tcgaccggat taaagccacg ttcggcgagc gcgaggtgtt
     1021 cagcgacgta acatacaatc ggctggtgga agtgctggac gagtggatcg ccactcgctc
     1081 gaacaataac gccctggagt tgtttgccga gctccgtcgg ttctggaagt tctgcgcacc
     1141 tactctttgc aatggccgca atgttgccgc cagtctgcca gatgattatg tttcctcccg
     1201 cgtacagaaa cccaccccca cacggctttt taccgatatt gaatcaatcg cccgactctg
     1261 gctcaatgtt gctgcctgca cctctgtaca ccagaagaat gctgttcgct tcatgatcat
     1321 cactggtgtt cgtccgatta atgtccataa cctgcgctgg gactacgttc acgaggaggc
     1381 tggtgaaatt gtttatccgg aaggggttat cggcatgcga ggggctatga aaacacaaaa
     1441 ggctttccgc ctgccgataa cgcctgagat ccggcggatt atcgacgagc agaaagcctg
     1501 gcgtgattca gttcctgagt gcaacaggga ttatgtattt ttgcagccac gtgatccaat
     1561 gcagccattt tcaaaacgat cactggataa gctggtgaaa acatacagcc cggacggggc
     1621 tgtaaaagga ataaaacatg atgggactgt taaagggaaa gacggtgcat ttaatacgat
     1681 gtgccgtaaa ttccttaaga gcaatgttat tgccttgatg aaggaaagag gctattcccg
     1741 ctcagaccga agggaaatca gcctcctttg ccttcaccac tccagcaagt cagatgaccc
     1801 gatggcagaa cattacgact tttctgatga gattttacag gaagagattg cgttgaagcg
     1861 cgaagctttc gaggctcacg agcggagcat acttgcgcag gtggcattgc tacggcggcg
     1921 aggttaatac tggctgcgac atttttgaat aaaagcgtcg acatttcggc gctcataacg
     1981 aactactttt gcactgaaac gaattggtgc caggatagcc cgatgacgat gcttaatatt
     2041 ccactcacat agcgttttct gtgtaatacc taacttttgg catacttcat ccggggtgag
     2101 taaatcgtcg ggtttctcgc tcatgctata cctctctttt tcatggcatc gagcaggatg
     2161 tcctgcactg ttcgttttga gttgcgccgt tccatcacca tttcgtccat agtgtcggcg
     2221 gcgataatgt ggtgaataaa taccggacga ttgtgtccgg cctgtatctg cctggtgggg
     2281 ccgatacgtt caataatttg ctgatattgc tccaggtccc accagtgtga gaaaaatacc
     2341 agtatatttc cgccgtcctg catgttcagg ccgtggcccg cgctggctgg gtgtgcaaag
     2401 agaacaggaa tctttccgga attccagtcg cgcagtgtct gtggatcctg gtcgaggtga
     2461 cgaccgcgag ggaatgcttt aagcaagcgt tcaagatcgt gtttccagtg ataagcaacc
     2521 agcacaggtg cgccagctgc ttcggtcagt atgctgtcca gcgcctgtag tttggtgtca
     2581 tgcagttctg accaacttcc ggtgtcgtct gtgtatactg cgccactggc gatttgaaga
     2641 cacttcagtg tctttgccgc ggcgttcggt gcttcgatgc cttcgccatt cagctcgagg
     2701 aacatttcct tttccatttc acgatactgc tgacgggcct tcggcggcat atccacgcgg
     2761 attacgttat ggatggggtc tttgatatcg aaccagtcgg ccgcatccag cgagagggtc
     2821 acatcggcta acgctcgctg tatttcagcc tgtgagtgag caaacggctc cagtttggtc
     2881 cagctctgcc ccggaaactg tatcgagttg aaccagcgtg aggtaaacgc gccgtaagtg
     2941 cgcccgagac gttgcccctg gtccacaaac cacaattgtc cccacaaatc taccaggccg
     3001 ttcggtgctg gcgtaccggt gagatttatc cagcgctgga catacttatg cgccactttt
     3061 cccagcgccg ccgcgcgctt accaccacct cgcagccgga aggattttag ccgggtgctt
     3121 tcatctggaa tgacagtacc gaacggccat cgttctccca attcctcaac cagccagaca
     3181 aggttatcgt agttgatggt gaacacgctc gcgttgctgt tcgccagcgc tgcagagcgc
     3241 gctttggcgt taccaacaat cggctgcacc tcgatattgc gcagatgccc ccatttcagc
     3301 gcttcatccg gccatgtgct tgctgcaacg cgtagcggcg cgaggaccag cgcgggctgt
     3361 gtctctgagc ccgccatgaa aagatcttcc agcgtggtga gtgtcgctac ggttttaccc
     3421 atccccatcc ctgcccagat gttgcagcgg gaaataccta tttcgtggtt gataattaga
     3481 tcttggtagg ggcgaggcgt gaaaatttgt cccatagtat tttagccagt agggcgcggg
     3541 gtaatcccgc gcaataatgt cagattagat cggtgtagta ggtgaagttg ccaaactgcg
     3601 gatgtgtcca gcgcttgcgg ttacctttcg gcggtgtcga ttcgtttaac agcttttgag
     3661 ctgcttgttc gatcgccgga aggttaacga gcaatccccg aacaccggtg tctgaacgta
     3721 ccggtatcct ggcgaagccg atcaatttcc ggcatgttga atcagataaa cctgttttcc
     3781 atgctgcttt gctgacgggg acatattcac cttcaacatg agataaattc gctggtgcgg
     3841 ccgattctgc ttgatatgtc ggaagggcaa tcacctccga catcgctttc atggttgctg
     3901 atgcggttgc ctccgcgatg acgcgagcaa gctggacaat tctactatcc acctgaatta
     3961 caggtgcggg gtgagtgata gctaactggg ggggctcgac ttcttttttc agtttttgcc
     4021 agtgcttaat catcgccata cgacgaacgg catcgtaacc tgttacaagg cattcggagt
     4081 gttcatagtc aagttggtac tcccatgcgt ttggattatc ggtattgtac tgcaggcaat
     4141 taacacactg attcgtaagg taaagcaaat cggctttacg gatataatcg cgctcatcac
     4201 cgccgtaaag ctttgccagc attgcacgaa tatcacggca aacatcggca tggtttttat
     4261 ttaccacttc tgctatctcg cgactgctca tcatcggttg cttaccagtg cctgctagtg
     4321 ttaaagttct ttcggacatt gaagtgttct ccattcagta aaagatgcgc tgatagttgc
     4381 agctaccagc gcgttttgtt tattcatccc acggcttgtt tgcagcctcc cttgcggcct
     4441 gctgtgcaaa ataaatcaat tcaaaagcta actgctctcc tttttccagc gaactgagaa
     4501 gtcttgctgt ttccagcatg tccgcgatat acccgaaaat atctgcctga tgtaactgcg
     4561 tgatctgaat agccatagcg ttaaccttgt tttgtactca ttgtgaggac tttattagta
     4621 ctcacaatga ggcttgaagt caacatgttt gtgaggcatg ttgttctcac actttcaagg
     4681 gatcatatat gagcaaaaga gacgacccgc agttgagggt acgcatacca gaattgctaa
     4741 aagaggcttt ggagaagaaa gcacgggcta ataagagaac actgacctct gagatcgtta
     4801 gccggttgga atctactatt cttcaggaca aattacttca tacatctagg gggtttgagg
     4861 aagctgctga tgagattctc atactcaggg atcttctcga gaaacttaag acaacgtacc
     4921 atcgagagta tcaggtcgaa tgggtttatg aaaacaaata cgagcttatt gaagtgatgg
     4981 aaaaacttaa gaagttgcta aatcctgaga atgaatagtg gattcatcct ctaaactttt
     5041 acgtaaccgc cgggtgattt cggcggttag tgttctgtcg ttttctctgg aaattttttc
     5101 cagtgtgtct ttcaagtctt gcgggatgcg tacccgcaat tgtgggtcat cacgtttact
     5161 cacaatattc cctccagatt tttgctatcc agcaccacca cggtaaagcc cagctttcgc
     5221 agacgttcat gctcgcgcaa ctggtcaggc cgtggtggct tgcctggcgc tttgcattcg
     5281 acaaagacga tgcgaccgcc gggtagcagg acaatgcgat ccggtaccga gcggcgaccg
     5341 ggtgacacga acttaaaggc gaccccgcca gcctttttca cttcggcgac gaggtgcttt
     5401 tcgataaggc tttcacgttc gtaggccatt tacttcacct gcttttcagc cctcagtacc
     5461 atctggctac catcatcaag accccagcta atttcgccgc cttctgccat taccagttgc
     5521 caaactaact gcgcggcttc gttcgttacg tcacgcccag ggtcattgcc aactcgcaag
     5581 cgaccgccgt caacatcgcg catttttgcc aacatgatct ttttggatag cggggagaac
     5641 cccagttgta gtttcgctgt attacgcatc attcttcgac cgccttacgc ttttcgcgca
     5701 tgttctgcat cagacaaaaa tcagaccggc gttcgctcca gtcctgattc agttcgttac
     5761 gtgactggcg gttagccttg gcccagacct tcgctgcccg gtcatattcg ccggactgct
     5821 caaggcgcaa ggcctccctc gcagccctgt aataaagtgg attgtcccga tatttaaatg
     5881 acataggggt tactcctgct ttgcttcgat tgggcggatg ctgctcagca ccagacgagt
     5941 gcggacaccg acagcaccac cgcggcggcc agtgtctttg tagtaatact cttttgggcc
     6001 agccacccac gttgtcgcgt tttgatgcaa ttgcacctgc ttttcaccgg tgcgggtaat
     6061 aaccgttccg gtatgcgttt tgattacagt catagcgatt aatccttacg gtagtggtac
     6121 gcctcaaaac cgccagcgtt cagtgggata tcgggcgccc attcggggtt attggagaga
     6181 agcgcggaaa gctctttatc gttgaaatct tctgtgtcag gtgattcggt gatcacctcg
     6241 tcgtgtaccg tcagcacaat gctgtaaccg gcatcttcga taagcggcat gtttccggcc
     6301 agaacgtcgc gggcggccgc ctgggtgacg ttctccacca gctttccgcc gtaggttttg
     6361 agtcgttgcc atttacgcga ataagagtta acacccatgt aggtgatatt cccttgttcg
     6421 ataaccggag acgggtagca tacagcgcgt ccggatggta gctgtatgcg cagccacgcg
     6481 ccatcgcggc ggattttaag ataaccgcaa tacaatgttt tttgcggtgt ggcgattgca
     6541 gtgcggacgg ttcgctccag ttcgtaccag aaatcgcagg tcgccgggtg cgccctgcgc
     6601 cacaggcgct tgagcgagtc acatgcgatg aatacacgct cggacaggcc aaaggtcgac
     6661 ttacgtttaa ccgattcgtt gtaccagctt ttcgcctcgc ggataacatc gcggggaatg
     6721 tttggcagtg cggcgttcgc cagctcgtcg agatcgagac cgtaaaccag agcaaaagtg
     6781 atgaaagccg atacaccacc tccataaccc agaccgagtt ccatgacttt accgatctga
     6841 cgcatgtgtt tatcaacatc atctggtgca atatcgaaag cttttgcata cgccagttta
     6901 tataagtccg gacccgttcc ggcgtcatac tctctgaatg cattcagttt ccattcttct
     6961 cccgccagcc atgccagcat gcggccttca atgttcgaca agtcacttac caccagtttt
     7021 ttgcctgttg gcgcgataat gcagccacgt aacgctgaac tggttagttc catgatattg
     7081 tcaaacagca ggtctgcaca tccggctttc agtgcttcga tgccttcgtc tatttgttcc
     7141 tgttttagtg aagggcgggg aaggttctgg ggctggaata gccgtccggc ccaacgaccg
     7201 gtacgtgacg ccccgcagaa ctgtagcgta ccgcgtaagc gcccgtcgtg gcttacgcct
     7261 ttcatcagtg ccttgtattt actggtgctg gtagtactgg cttgcaggcg gatagccagc
     7321 agttctttca cggcagatgg taaatggggg tcggcaatac gacgttccag agtactgcgt
     7381 tgcatgtctg gtagctccac accgtaggat tcaacaatgt gcttaatcaa cgcgtctcgt
     7441 tgtgtggctg cctgcacttc gccatcagtc atttcctgtg tacgctttgc caggcgcttt
     7501 tgctcctggt ctaccgcttc gatcgcagcg cgcgcgagtt gcatgtccat gcagacgccc
     7561 cggtcgttga tctgctgatc acgatgccag agcgccagct ctgtcccctg ataattccac
     7621 ttcggcagac gtttatagac ttcgcgcatt gcctcgatat ccagtccggc gtaagcaaca
     7681 aagcgccgcc attcttccgg gtgggtttta ctggtggccc gacgcagttt gctgtttttc
     7741 gggcgtggct tacagaacag ctggatcagc gctttacctt ctttgtcctt cgctttgtct
     7801 tgcgggacgc ctagtacttc gcagagttcc cccagagacc ccgggagacc gtgcgccaac
     7861 gcctgcacca tcgtgtcgcg ccaacgttcg actggcggtg ccagtcgcgg tattgcataa
     7921 cgcagaacgg tgcggtcgaa gtgagagtta tggaaataaa gcagggtttc agggtctgcg
     7981 attgcttcgt ataagccgtg tggaatacca ccaccggcag tgatatccca cacgtttact
     8041 ggcccgtcgt tgatagccca tgcgaaaagc atcacttcaa cgccttcggc atacgcatgg
     8101 gtgccattat tgataggtat ttcacagaaa gtttccaggt cgccccatag tatattggac
     8161 attgtattta ctccgaagaa ttagctacat ggatacgaaa gatattatct cacttgttgg
     8221 agtcataatt tcagccataa ctgcaccagt cggtgtttat aagttattta aagatgctag
     8281 ttggttttta ccgaaaacaa caaaattctc gcatattctg aagaattatc cggagcatat
     8341 tgagccttct gatgtagagt ttatgaaggc agaaatcaaa agagaagtta aaaaaagtat
     8401 attaggtata agtagccaga aattaagaac gttagttatt tatgtcagaa cgtattctga
     8461 attgaaaatg ccgttttggc agtggggtta tttagcaccc catattcagt gtaaatacga
     8521 tagattcttt atacgctaca aaggtaaata caaacgatgt cgctggtgtt ctaaagttgt
     8581 ttctatcttt tatttggcag atggcttgat atttttctgt tggtcgttaa attatggcgc
     8641 tgtttttata ggaatgggcg ctgttttaat gttgctgtgt atatggatgg cttttatgtt
     8701 ttggtttctt tttccgggac gaggcgttat aaagaagtat aattcccaat tattgaaaat
     8761 agatgcaagt aagtatcagg ccaaatgata ttacaggtct gatactttgc ttttttaaat
     8821 cagtgttttc agacaaaacg aagtcctccg taaattaaac ggtgataaaa agcccctggc
     8881 tgagcagggg cgaactgaag gattaacgag gtaaagtggg tgggatatat ctgccgatgc
     8941 ggtcttctat ggtcggcaga atgcacatgc tgtcgctgaa gcggtcatgc aactggtaaa
     9001 ccagtttcga atccattttt tcgagagcag gcattaactg cgcccgccag atttcgttga
     9061 tgcgccggaa gttatgacag acggcgcgga gattgttgat gaacatttcc ttctctgcgt
     9121 cactgactgc tggctgcgct ttctgaatgc gtttcgtgct gccattccgg tagaagcgtt
     9181 cctgagctgc aacagcctca cccacggttg gatagcaccc gacgtaaacg cttttctctc
     9241 cgtcccatgc ataagccctg tacggattgt atttgctccg gtgatggtaa accattttcg
     9301 gcagacactc cctgtgcggc gccagtatcg tggaggattg cggaacagcg gtttctttct
     9361 cgataaggtc aagcacccaa cggcggaatt cttttgctac cggagttgtt gcgaacatag
     9421 ctaccaggtg ggcaccgcgg agggagaaaa tgcggcgcga ggcctgtaaa ttccccgagg
     9481 taaccatttt gattacctcg gacatgccga gagagaactc atccgtgttg cgggtgtaaa
     9541 tgttagttac gctgcgagca tccgaatatt ccagcgcggt agctagggta gccgcagtaa
     9601 accaagtttt gccgttatgt tcgatcgttt cgagaacgtg ggatttgaat acgatttggg
     9661 tactcatgac gattttcctc ttaactgagt taatcgccac catcaggtgc taatcatcgt
     9721 ggtggcgaac tgtgcggggt tagcactacc gggttaagag gaaccggcgc ggatttctcc
     9781 gcccccacac agcccgccat aatgcgaacg tggcaaagct tgcgacaata aaaagacgct
     9841 ggcgcgtctg gtgtcgcctc ttaaacttcc ggggtgctaa tcccgatctc catttgggag
     9901 attcggtgag cataaaccgg aagggggcga tgagtcaata tcggggtagc agggcgactg
     9961 ggtgctaacc agaacaatac aggtagctcc gggcatattc ccctttgcaa agacacccgg
    10021 cgtttaaccg ggcgtctgtg aagcaggatt aaatcagtgc ttcagcatca gcaccttcgc
    10081 tgatatcgtc gaaatcgtca gcgcttgcta ctccgccgcc agcgaatgca tcgccgtctc
    10141 gcaggaactg gactccgccg agtgaggcat taatgcgttt accgaaatta ttgtcctgtg
    10201 cccagatatc gataacggcg tttacatagc accctgcata gggacgtcca tcagcctgaa
    10261 ttagtggcga acgatcgcga tcaagaacgg ccgggcgcgc tttgttagca gcattcagga
    10321 agaaattacc ggggaagcct tcatactctg ctttttcatc accatcatgc aggcacagat
    10381 tgagtttttt ctccagctgg ttataaatgg actcccactt ctccccccat ttttccttcg
    10441 ctacctgctt catagcttta cggatttctt ccagttgtgg gtgtttggga gatattaaaa
    10501 atactgcgga gaaacgcgga tcgccttcgc cgtttacagt tttagcttca aacagagcag
    10561 gaaaagctaa tctcacgtta tttaatttaa tcttcatgaa agtctccttt aatcagatga
    10621 ggtctgcggc gagcgtatcg tcggatacgt cgtcgaaatc atttacaggg ttgatattga
    10681 gtgcggggcg tgggtccgac tcgggaacga cggttggttt accatcagct cgtgttatca
    10741 gtgcctcgac ttttgaccaa cggcgcggac tggccttttt gataagtttt tcggctttgg
    10801 ttgggctaat cagcttaaga tcgaaaacct cctcggtttt ataacggaac tggtctttca
    10861 gcagcgcgcg ggcggcttct tcatcactcc aggcccggtt accttgtttt cctgttacca
    10921 gtttaaaccc cggtaccgga tttccggcat tgagttcatt atgaacccgg tcccgtactg
    10981 cctttagcca ggattcaata aagtcggcct gactatagat ctccgcaagc tgctcaatgg
    11041 ttaacagagg tacacgtgcg ctggcattgg tgatgatttc gctgacaggc tttgtcagat
    11101 cttcaaaatc gctggccgct gtttgtaaat gctgcatttt ctgggcagtg caaatagctt
    11161 ttgctttaca gaagcggcac tgtttttctc caggtatgaa gttttccagc ggtagtgtct
    11221 caatgccttc gcattcagca atattgagaa caaggatcgc actggttgcg gcctcctgtg
    11281 ctcgtttacc gaaagactga agctcctgta cggttaaaga ccattctgaa acgtggttga
    11341 gccttggctg gtgaataaat aatcttacag tctcaaagtc atacagcatg ctgaattgtt
    11401 cgagagcacc cagagcatac agttgtagtt gctcattttg ttctgcatca atgcggacgc
    11461 ctttgccata tttcaggtcg tggatttgta attcgttacc agcgatgatt atgccgtcgg
    11521 cagttccgaa agattcttcc acacccgtta tatgtgagaa atcaacacgt tgttcaacca
    11581 atagttcatt attctgtgca agagtccaga ccgtatcaac ataccggcca acggcttcga
    11641 ccatttcatc atccacctgt gggccagatg tatcatcagg attttcgcga agggggtatg
    11701 agccgagaaa catagaaaca ttgcatccgg cgtagtgttc cgggtggctt tgcctgtttc
    11761 gtagaacttt ttcagcaagc gcgtgcgctg cagtgccctc gattgcaaaa gttgtttctt
    11821 tatccggttg tgtggcctcc agcgccagac tacccgcaca ccttagccaa cgatgtgcag
    11881 atgatggtga taatcgtgca tgaacatcag gcattcatga cctcctgaat gtgttttcgc
    11941 cgcgcagccg ctacgagttg tttggtgggg aaatgaccga gagatttgaa tttgccgttt
    12001 aatcgtccgt aggcctgcca cgggtttttc ttaccttcgc tgaaccgaac gccaaccact
    12061 ccagatttat tttttctggt gtgagagtta gccctgtttt gtgaaagaga ggcccagcga
    12121 agatttaccg cccggttgtc atctcgacga ccgttaatgt ggtcaacttc aggggcagat
    12181 ccgggaataa atgcagcggc aacgagacgg tgtattttga cggttacctt tttacccgct
    12241 ttccataagt cggtgatgag atatccgtct gcgtcatagc gtggttttag tgacttacct
    12301 ttaatgggat aggtgcagcc agaacctttg tttaatacag ttctgtcgag gcttctcagg
    12361 ttgccagcgt ctgaaatctg gtaaattcct tcaaagcctg aaatatcctt ccaggcttcc
    12421 ggcatgatta accctccagt gctttttcag ccagggtgat tacttcagcg agattttcat
    12481 ctgttacttc accaagtttc ctggctccct gtttttccag aattgcaata gcttctgccc
    12541 ggtaaccccc ttttgctaac tggaggatca acccttcagc ttgtttgcgt agtgccgcga
    12601 aatcaattgt atggtcatct ttggcgtcat tattctggct ggaatttgct gcgtctctgc
    12661 gtgcaaattc ttcctgcagc tgaaggtact caacacggtt gatctcgata tggccttttt
    12721 tcagcatctc gttcaacttg cgtaaggtgt ggagttcact ggctgctgtg ccggatacat
    12781 ttttgacgta aaacggcccc gtgcgttctc catctttgtt actggccttt ttcggcttaa
    12841 cttcatcacg cccatctgca ggtgcatcaa gtagctgctc ggcaaaagca cgtcgctcgc
    12901 cgatggttgg caggtcgtcc cagaacttaa gaatgttacg ggacaggtcc aggagagcag
    12961 gtttaagcag cgccctggct cgtttgacgc cctgtaatgc gctgtcgaga gcatcaatct
    13021 gaactactcg tttatcgcct tcagcatcac ggtaggcaac agcacgttgc agcatgtctt
    13081 ctgtgatagg ggtggctacc gggtagaaac cagccagtgc gataacgtcg ctgaactcca
    13141 gatcatccag tgtcattgcc gctgacatgt tttcagcttc agttgctgta tcccgacatt
    13201 cctgcactcg tgaaatcgtg tcaggatgca taacaatacc tgatgccatt gtgcggataa
    13261 gacgttcaag cagcgcatta tgttgttcca gaagttgatt attaagttcg agactggttt
    13321 ctaaactcat actgtggtcc tcgctacaag gagaatgaaa gtgatgatca gaccgagcgc
    13381 agtggcaacg gccagaccgg tcatcaaatc gaagttttta cggcgataac ggagaacatc
    13441 gcgccccgtc agtcgataga ggtgttcagg tttcatcggt tgtgctccta aaacgcccat
    13501 gcgctttttc ggctcggata cgtgcggcaa tggcgctatc aagttcgggg aaatacccga
    13561 ggtggagtgg tttaccgtta acggtgattt gtgcccgcca acgctttctt gattctttcc
    13621 agttgacccc cattgcgttt tgtggggcta cgctgtgcaa ggtttttggc gttctcagcg
    13681 cgtgtgacca gacgtaagtt ttcaatgcgg ttatccgccc ggttatgatt gatatggtca
    13741 atttccatac ctgcggggat atcgccgaag tggtattccc agacgaggcg atgaacgtac
    13801 tgattttttc catcaaggca gatggtgaca taaccgtctg cttttacttt tcccgcgggc
    13861 ttgcctgcca tgcgtgaatt agtggatttc catgcgtggg ggcttttgaa atgcgatgcg
    13921 ggtcggtatt tccatataag ctggccgttc tggtattcaa aagcctccct gattctcttt
    13981 ttcaacgtag gagtcctcgt gaaggtagga atgaaaaaaa gcccgtcatg ggaggcgggc
    14041 aaagactaca catagcaatt acatggatga ttcagaagaa ttagttagcg gacggattgt
    14101 gtagccataa ccgagatagc cgttgcgttc ctggtaggcc gccgctctta gtcgggctgg
    14161 gttttcgcct ttgacggttg caacgacgta atcagtcgcg tcaggtccgt tttctaccac
    14221 ttcaaaagcc ggagcgtatt ttgttgtgag ctcataggta ttcatttcgg taccccttca
    14281 gtggattagt aaaaggcccg aagcctttga ttaattcact gcacgcccca tcatcggggc
    14341 gtttcaattt acgtgactta tcagctcgtc gcggtgttgt cctctacgct taccgtacgc
    14401 atacggactc ggcgcttacc tcgatcccat cgggtgccat ttcattttgc caggagtact
    14461 gcggcttacc tgtcacgcgg ttcagtttgt taaagagcga ttacttagtg cggttaaaaa
    14521 tacaagcaaa cctgtaagtg cgtcaacagg tattctggta ataaaatgca ttattaacct
    14581 aattacttgt ttttacaggt aataaaaaaa gagggcctaa gccctctttg tttagtagtc
    14641 aggatgcgat tatctttttc tgcggaatat gcgatgttct accattgtcc ctatgatctt
    14701 aattggtttt tcccaggagc gacaaatagg gtaatcacta ttcaatggaa caagttcaaa
    14761 gtcttcctca cccttagcgc caatccctac cgggcggtat tttttgaacg ttgcttcgtg
    14821 gctaccgtta catgccgcaa cgaattcacc aggagtaggg tatacgtcag tgtcaattat
    14881 aacgatgtcc ccctctttaa attcaggttc cattgaatcc ccacgtatac gtagcgcaaa
    14941 tgaactttct gatagctcag ccgtggtgag aatgtattcg aaatcacctt cttcacgaag
    15001 tagctcagcg cttgtgaaag cccctgcttg tacgtagctt agaacaggca cccttcgggc
    15061 tccgtagttg aaatctgtaa caacatcgcc cccaagaagt agccatttag gatcgcattc
    15121 aagagctgac gctaaagcaa gaagattgcg tggtttaagt gttttcccgt tctctatagc
    15181 ttcaatcgac tgctggctaa tgcccgctcg tttcgcaact tcgacctgag ttaagtttag
    15241 ttctgttctg cgttttttag ctcggttggc aagattcatg ttgactcctt ttctgtcatt
    15301 gatgattaca gatacttctg tatttgacaa acaaggttgc ctgtgaggta attacaagta
    15361 aacctgtaat aatgagggct tactcaatga ccagttccac ccttgcttca cgtataaagg
    15421 agcgccgcaa agctttaggt ataacccaaa cttcattggc tgaaagtgtt ggcatgcgcc
    15481 agcagtctgt tcaatacctt gagtccggac gcgccacacg taccggtttc atccttgaat
    15541 tggcaaaagt tctgaaatgc gatcctgact ggctattaaa cggcgaaaat tcagagcagc
    15601 aaaaggtgta acccatgcct gataacaaac cctggggagc tacgcccgat gagtggttcc
    15661 acttcgatct ggtgttgggg atgacagaca ggcttttacc tgtggtgtcc aatccctgtg
    15721 cagaactgtc gcctaatagc aagctgtctc ataatttcgg caaaactccc agtcgctaca
    15781 atggccaacg taaggttgtt ggtatccccg actgggctaa ctatgttgtt acggaaaacg
    15841 attttgcccg gtggtcgaac gaacccgatt acggtatttg cgttcgtact ggcgacgggg
    15901 tggtggctct ggactgcgat atcaatgacg ccgggatgca ggagatcgta cgcaatatta
    15961 tcctgtcatg cctgggggaa cttccgccac gtcgctggcg ggctgacagt cataaatgcc
    16021 tgtacctgat tgctgttgat ggcgactacc gtaaacgtgg tcaccgcctg gaaggcgaaa
    16081 acaaacaaat agagctgctc gctaagggcc agcagtttgt ggcttgtggt acccatccgg
    16141 caggcgaacg tattcagtgg gactgcggtt tgcccggcga acctctgaaa attacatctg
    16201 aacagctcga aagcctgtgg cagcgtctgg cggataactt acccgtgaaa gacagctata
    16261 ccgcaggtgc aggacgtcag cgtgaccttt cctgtgttga tccttccgcg acagatgatg
    16321 tcgctgactg gcttgacgcg aacgggtgga ctctgtcagt cagctctgat ggttcccgca
    16381 atctgaaacc gtttcgggat gagtcagaat acagcaacgg ttgcagcgaa acatccatta
    16441 agtattttcc aaaagggacg gggggatttg aactgggaca ttttaaaagc atgcacaaca
    16501 ccgatgcggg gcttactgat gccgactggc tggatgcata cggctaccgc gtggcggact
    16561 tcgaagattt gacagtggct gaagatggcg ataagcctga gtatcttccc tggcctaagt
    16621 tcagtcgtga taagaatgac cggatagaag gtaccgttac caatgtgctg ctggcgctgc
    16681 ggcgaccgga tctctgcggt gtacaaatcc gccttgatga gttccgggac gagataatac
    16741 tgatcactcc acgggggaaa aacctgcggt tgcgtgatga gttttatacc atgctgcact
    16801 ctaggctcga atgtctggga ttcaagaaat ttgaagaggc agcgatcagg cgtgcggtgc
    16861 gcctggtcgc gtacgaaaac cgttttgatt cgcttaaaga ctggattgcc aatcttcctg
    16921 catgggatgg tgttccccgt atagatactt tcttctgtcg gcactggggt atcgaggcca
    16981 acgcctacac ccacgcagtc gggcgctact ggtggacttt gctcgcgggt cgagctctgg
    17041 ttcctggcat taaaggggac atggctgtga tccttgtcag ccaacagggt aagaacaagt
    17101 ccgaaggtat ccgtgcaatg gctccaaccc ctgagcatta catggaactg gacttcggta
    17161 agaagggaga ggagcgcatt cgtgagatgc gagggcataa cgttattgag cttggtgaga
    17221 tgcgcggtat ggataaacgg ggtattggtg atgttcgtgt caccatatcc acccgtgcag
    17281 accgcaaccg cgggttgttt cgggagcact atgtcacttt accgaggcgt tgcggtttta
    17341 ttggtacttc taactccgat acgccattga cagataccga aggtaaccgc cgttggttgc
    17401 cgatgacgat accggatgag actgacggca agtttatcgc tcagggtatc gctgacgaaa
    17461 aaatacagct ttgggctgaa gcagtcgccg tttttaaacg tgatggcatc gcatgggaga
    17521 acgctgaaac actggctaag tctattcttg gggattatga ggtcaaagat gaggtctgga
    17581 tgtcatgcgt cgctgagtgg ttagagttgc cacaggatct gtacgatatg gctgccgacg
    17641 gcacgaaaaa gaactgcgaa cgcgtccccc ttacgtcaaa agacgtattg gtcgaggcga
    17701 ttggcttcaa agccccccag gtaaaacgca gtgacgaaat gaaaattgca gcaatcatga
    17761 agaagcttgg ttacaaaaat aaacagttgc aaagtttacc gggtaagcct cgcagctggg
    17821 aaaaatgcaa ataactacaa gtttgtagat ttttgcaggt tgttactcaa cctactcaac
    17881 ctcacaaggg taggttgagt aagaaaaatg gtattaaaat catgatatta ctcaaccact
    17941 caacctactc aactatttta tataaatata cgcgtgcgcg cgcacgcatg tatgtaagaa
    18001 ttttattttt ccaaaaggtt gagtaggttg agtaagagta tgatttttat gttttttagg
    18061 ttactcaacc ttttgcccgt aaaaaaggtt gagtaaagaa aacttgcaga aaatcacaaa
    18121 taaccaaacg aagtaaaaac atgaatacaa cctgtgcaag attaccaagt gcggatacgt
    18181 tagcggaaca ggcagcaaaa acggcaagtg ggtggatgtc agacgccgta atagcgatag
    18241 atgagctttt tggaaagggc tatgcgaagc aacaccctga attagttgct gcctttatgc
    18301 aaacagcggg cttagatgaa gctgcaatgc acttacgggg acttgtgtta gctaaccaac
    18361 ggattgatga cacactggaa gtgctttcgg agcatattat gctgcttaac gagccccaat
    18421 aaacccgatg cggataaaca gacgtagttc tcagaaaatt ttttcgtagc aaaacgtaga
    18481 ggccagcgtt atgcgtagag atatgcaact tgttctggag cggtgggggc gatgggcggc
    18541 gagcgaggaa tactgttcaa tggttgactg gccctcaatg tcggttactt cccgggacgt
    18601 aaccgatagt ggcaaacccg gttgctctga cgaggacgga gcggtgattg atacctgcat
    18661 cgcgcatatg agtatggttt gcccgtacga tgatttgctg atcctggggt tacgttttat
    18721 aggcggcctc tcaacgcgtg caattgctga ggcgatagac cgtagtcatc ttagcgtacg
    18781 gacatccctg aaggcgtctg aagcatttct ggaaggagct ctgactctgc agggagtaag
    18841 gttggatatg gatcctgaag ttgtactacc tgaaagggtt gtgtgcgcac aaaaaaatat
    18901 gttatggtta tagcgtctat aattgtatgc ttactgctga aaggttctga taacaccgga
    18961 accttttttc ttgtcttctg ctgggaaaaa ttaataaaac aggaaagaaa aacatgacat
    19021 cgcactggtt cagtgtcagc cagtggcagt taccaaacga ggatgattac agaaaacttc
    19081 atgcgttgtt agctcagccg gacagagcaa ttgccttcta agcaatcggt cactggttcg
    19141 aatccagtac aacgcgccat atttatttac caggctcgct tttgcgggcc ttttttatat
    19201 ctgcgccggg tctggtgccg tattacttca gccaaaagga atacctgtat atg
//
LOCUS       DAZKFU020000075        19200 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZKFU020000075 DAZKFU020000000
VERSION     DAZKFU020000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.075"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(32..1486)
                     /gene="xylB"
                     /locus_tag="ACHOQQ_RS10490"
                     /old_locus_tag="pgaptmp_002098"
     CDS             complement(32..1486)
                     /gene="xylB"
                     /locus_tag="ACHOQQ_RS10490"
                     /old_locus_tag="pgaptmp_002098"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGU4470352.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1558..2880)
                     /gene="xylA"
                     /locus_tag="ACHOQQ_RS10495"
                     /old_locus_tag="pgaptmp_002099"
     CDS             complement(1558..2880)
                     /gene="xylA"
                     /locus_tag="ACHOQQ_RS10495"
                     /old_locus_tag="pgaptmp_002099"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGU4470353.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3246..4238
                     /gene="xylF"
                     /locus_tag="ACHOQQ_RS10500"
                     /old_locus_tag="pgaptmp_002100"
     CDS             3246..4238
                     /gene="xylF"
                     /locus_tag="ACHOQQ_RS10500"
                     /old_locus_tag="pgaptmp_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4470354.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            4316..5857
                     /gene="xylG"
                     /locus_tag="ACHOQQ_RS10505"
                     /old_locus_tag="pgaptmp_002101"
     CDS             4316..5857
                     /gene="xylG"
                     /locus_tag="ACHOQQ_RS10505"
                     /old_locus_tag="pgaptmp_002101"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGU4470355.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5835..7016
                     /gene="xylH"
                     /locus_tag="ACHOQQ_RS10510"
                     /old_locus_tag="pgaptmp_002102"
     CDS             5835..7016
                     /gene="xylH"
                     /locus_tag="ACHOQQ_RS10510"
                     /old_locus_tag="pgaptmp_002102"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGU4470356.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSTAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7094..8272
                     /gene="xylR"
                     /locus_tag="ACHOQQ_RS10515"
                     /old_locus_tag="pgaptmp_002103"
     CDS             7094..8272
                     /gene="xylR"
                     /locus_tag="ACHOQQ_RS10515"
                     /old_locus_tag="pgaptmp_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGU4470357.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8380..9204)
                     /gene="bax"
                     /locus_tag="ACHOQQ_RS10520"
                     /old_locus_tag="pgaptmp_002104"
     CDS             complement(8380..9204)
                     /gene="bax"
                     /locus_tag="ACHOQQ_RS10520"
                     /old_locus_tag="pgaptmp_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGU4470358.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9524..11554
                     /gene="malS"
                     /locus_tag="ACHOQQ_RS10525"
                     /old_locus_tag="pgaptmp_002105"
     CDS             9524..11554
                     /gene="malS"
                     /locus_tag="ACHOQQ_RS10525"
                     /old_locus_tag="pgaptmp_002105"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4470359.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11732..12985
                     /gene="avtA"
                     /locus_tag="ACHOQQ_RS10530"
                     /old_locus_tag="pgaptmp_002106"
     CDS             11732..12985
                     /gene="avtA"
                     /locus_tag="ACHOQQ_RS10530"
                     /old_locus_tag="pgaptmp_002106"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGU4470360.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLHEKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13136..13609)
                     /gene="ysaA"
                     /locus_tag="ACHOQQ_RS10535"
                     /old_locus_tag="pgaptmp_002107"
     CDS             complement(13136..13609)
                     /gene="ysaA"
                     /locus_tag="ACHOQQ_RS10535"
                     /old_locus_tag="pgaptmp_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4470361.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13711..13851)
                     /locus_tag="ACHOQQ_RS10540"
                     /old_locus_tag="pgaptmp_002108"
                     /pseudo
     CDS             complement(13711..13851)
                     /locus_tag="ACHOQQ_RS10540"
                     /old_locus_tag="pgaptmp_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            complement(13853..14668)
                     /locus_tag="ACHOQQ_RS10545"
                     /old_locus_tag="pgaptmp_002109"
     CDS             complement(13853..14668)
                     /locus_tag="ACHOQQ_RS10545"
                     /old_locus_tag="pgaptmp_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4470362.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            14894..16294
                     /locus_tag="ACHOQQ_RS10550"
                     /old_locus_tag="pgaptmp_002110"
     CDS             14894..16294
                     /locus_tag="ACHOQQ_RS10550"
                     /old_locus_tag="pgaptmp_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4470363.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNQLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            16305..18275
                     /locus_tag="ACHOQQ_RS10555"
                     /old_locus_tag="pgaptmp_002111"
     CDS             16305..18275
                     /locus_tag="ACHOQQ_RS10555"
                     /old_locus_tag="pgaptmp_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HGU4470364.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNISRVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIVVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(18405..19145)
                     /gene="yiaT"
                     /locus_tag="ACHOQQ_RS10560"
                     /old_locus_tag="pgaptmp_002112"
     CDS             complement(18405..19145)
                     /gene="yiaT"
                     /locus_tag="ACHOQQ_RS10560"
                     /old_locus_tag="pgaptmp_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGU4470365.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT         4784 a         4962 c         4757 g         4697 t
ORIGIN      
        1 atcccccacc cggtcaggca ggggataacg tttacgccat taatggcaga agttgctgat
       61 agaggcgacg gaatgtttca cgtcgtggct gataagcggc ataacgctgc gcatctggta
      121 gatgcgactg ttctaacggt agttgcggca acaactcaat gagcgatttc tctggattcg
      181 ccgcaatctg cgccagcctt gctgcgccca gtgctggccc cacatccccc cccgtacggt
      241 aatcgagctg ctgaccgctg atatccgcca gcatctgacg ccagtactca ctacgcgccc
      301 cgccaccaat caacgtaaca ctttgcggtt taatgccgca ggcatgcacg acatccatgc
      361 catctgccag cgcataaccc acgccttcca gcactgctcg cgccagttca ttggggccat
      421 gttgatgagt caaaccaaag aaaacccctt tcgcctgggg attattgtgt ggtgtacgct
      481 cgccggaaag ataaggcaga aaccaaactg gctcggcact ttcatcagcc tgttgagctg
      541 cagcgattaa agctgggaca ttgctcaggc cggttaattt tgcggcccaa tccagacacg
      601 acgctgcact cagcattaca gacattaaat gccaacgctg cggtagcgca tggcaaaagc
      661 tgtgtacggc gctttctggc ttgcttaaga acccttcgct gacagcaaaa tagacccccg
      721 acgtccccag cgataacatt gcctgattag catcaaccat tcccacacca accgcaccag
      781 ctgcattgtc gccaccgcct gcgataactg gcaccgccgc cataccccac gctttcgcaa
      841 cttcaggtaa caaagcacca gtaatttcgc tgccttcgta taatgcgggc atctggtcac
      901 gagataagtc gcaagcctgc agcatgacgt cactccagtc acgctttgcg acatccagcc
      961 acatggtgcc agctgcgtca gacatatcgc tggcaaactc tcccgtcata cgcagacgca
     1021 agtaatcttt cggtaataat actttgtcga tttgacggaa tatctccggc tcatgccgct
     1081 gaacccatag caatttaggc gcagtaaatc cgggcatcat caggttgccg gtaatcactc
     1141 gtgattgcgg aactctcgct tccagcaaag tgcactcttg cgcacagcgc ccgtcgttcc
     1201 acaaaatggc agggcgcaat acccgttgtt gagcatccag taaggttgct ccatgcatct
     1261 ggccggcaat acccaatgct ttaacgtcct gcagagaatg ctgatcgccc agagctttca
     1321 ttgcgcgatc agttgcctgc caccactgtt ccgggtcttg ttccgaccag agtggatgcg
     1381 ggcgcgaaac ggtcagcttt tccgtttgcg aagcaaccac ctcaccctgc tcgttaagca
     1441 aaataacttt tacgcccgag gtgccaagat ctatcccgat atacatatcg atcgttcctt
     1501 aaaaagatgc ccggtatcgc taccgataac cgggccaacg gactgcacag ttagccgtta
     1561 tttgtcgaac agataatgat ttaccagatt ttccagttgc tcctggcgac cactctgatg
     1621 caccggagac aaattatgtt cctgagcata tttggctaaa tctgccagtg acatttggcc
     1681 tttcaggatt tgctggccca attcgctatt ccagccggaa taacgctgcg cgatgcgttt
     1741 atccagctcg ccatcttcaa tcatgcgcgc tgcaattttc agcgccagtg ccatcgtatc
     1801 catcgcgccg atatgaccgt aaaacagatc atatttatca gtactttgac gacgtacttt
     1861 ggcatcgaag ttcagaccgc cggtggtgaa accgcctgct ttgagaattt catacattac
     1921 cagcgcattc tcttccacac tgttcgggaa ctgatcggtg tcccagccca gttgagcatc
     1981 gccacggttc gcgtcgacag aaccaaacag gccaagcgca atggcggtgg caatttcatg
     2041 atggaaagag tgacctgcca gcgtcgcgtg gttagcttca atgttcagtt taatctcttt
     2101 ttccagacca aactgtttca ggaagccata gactgtcgca gcatcgtaat catattgatg
     2161 tttagtcggt tcttgcggtt tcggttcgat aagcaacgtg ccctggaagc cgattttatg
     2221 tttatgctca accaccattt gcataaagcg gcccagttgt tcacgctcct gacgcaaatc
     2281 ggtatttaac agcgtttcgt aaccttcacg accgccccac aggacatagt tttcaccgcc
     2341 caatttatgg gttgcttcca tcgctgtaac aacttgcgtt gccgcccagc tgaagacttc
     2401 tggatctggg ttcgtcgccg cacccgcgcc gtagcgaggg tttgtaaagc agttggcggt
     2461 tccccacagc agcttcacgc cgctctcttc ttgcttgcct gccaggacat caaccatttg
     2521 cgcaaaatta ttgatgtact cttttaacga cgcgccctca ggggaaacat ccacatcgtg
     2581 gaagcaataa aatggcacat gtaacttgtg gaaaaactca aatgcgacat ctgctttacg
     2641 cttcgccaac gccagtgcct caccaggctg ctgccacgga cgattaaacg cccccacacc
     2701 aaacatatcc gccccgttcc agcagaaggt gtgccagtag caggcggcaa atcgcaagtg
     2761 ctcttccata cgcttgccca acaccagttc gtcgggattg tagtgacgga atgctaacgg
     2821 gtttgaggat tttgagcctt cataacgaac gcgatcgagc tggtcaaaat aggcttgcat
     2881 attgaactcc ataatcaggt aatgccgcgg gtgatggatg atgtcgtaat attgggcact
     2941 ccctttcagt tgctcaatta tgttatttca cactgctatt gagataattc acaagtgtgc
     3001 gctcgctcgc aaaataaaat ggaatgatga aactgggtaa ttcctcgaag agaaaaatgc
     3061 aataagtaca attgcgcaac aaaagtaaga tctcggtcat aaatcaagaa ataaaccaaa
     3121 aatcgtaatc gaaagataaa aatctgtaat tgttttcccc tgtttagttg ctaaaaattg
     3181 gttacgttta tcgcggtgat tgttacttat taaagctgtc ctctaactac agaaggccct
     3241 acaccatgaa aataaagaac attctactca ccctttgcac ctcactcctg cttaccaacg
     3301 ttgctgcaca cgccaaagaa gtcaaaatag gtatggcgat tgatgatctc cgtcttgaac
     3361 gctggcaaaa agatcgagat atctttgtga aaaaggcaga atctctcggc gcgaaagtat
     3421 ttgtacagtc tgcaaatggc aatgaagaaa cacaaatgtc gcagattgaa aacatgataa
     3481 accggggtgt cgatgttctt gtcattattc cgtataacgg tcaggtatta agtaacgttg
     3541 taaaagaagc caaacaagaa ggcattaaag tattagctta cgaccgtatg attaacgatg
     3601 cggatatcga tttttatatt tctttcgata acgaaaaagt cggtgaactg caggcaaaag
     3661 ccctggtcga tattgttccg caaggtaatt acttcctgat gggcggctcg ccggtagata
     3721 acaacgctaa gctgttccgc gccggacaaa tgaaagtgtt aaaaccttac gttgattccg
     3781 gaaaaattaa agtcgttggt gaccaatggg ttgatggctg gttaccggaa aacgcattga
     3841 aaattatgga aaacgcgcta accgccaata ataacaaaat tgatgctgta gttgcctcaa
     3901 acgatgccac cgcaggtggg gcaattcagg cattaagcgc gcaaggttta tcagggaaag
     3961 tagcaatttc cggccaggat gcggatctcg caggtattaa acgtattgct gccggtacgc
     4021 aaactatgac ggtatataaa cctattacat tgctggcaaa tactgccgca gaaattgccg
     4081 ttgaattggg caatggtcag gagccaaaag cagatacctc actgaataat ggcctgaaag
     4141 atgtcccctc ccgcctcctg acaccgatcg atgtgaataa aaacaacatc aaagatacgg
     4201 taattaaaga cggattccac aaagagagcg agctgtaagc gttacgcccc agcgcggagc
     4261 gggggcgtga tttctctcca tgccgcgtga atgaattggc ttaggtggag tcgttatgcc
     4321 ttatctactt gaaatgaaga acattaccaa aaccttcggc agtgtgaagg cgattgataa
     4381 cgtcagcttg cggttaaatg ctggcgaaat cgtctcactc tgtggggaaa atgggtctgg
     4441 taaatcaacg ctgatgaaag tgctgtgtgg tatttatccc catggctcct acgaaggcga
     4501 aattattttt gcgggagaag agattcaggc gagtcacatc cgcgataccg aacgcaaagg
     4561 tatcgccatc attcaccagg aattggccct ggtgaaagaa ttgaccgtgc tggaaaatat
     4621 cttcctgggt aacgaaataa cccacaatgg cattatggat tatgacctga tgacgctacg
     4681 ctgtcagaag ctgctcgcac aggtcagttt atccatttca cctgataccc gcgttggcga
     4741 tttagggctt gggcaacaac aactggttga aattgccaag gcacttaata aacaggtgcg
     4801 cttgttaatt ctcgatgaac cgacagcctc attaactgag caggaaacgt cggttttact
     4861 ggatattatt cgcgatctac aacagcacgg tatcgcctgt atttatattt cgcacaaact
     4921 caacgaagtc aaagcgattt ccgatacgat ttgcgttatt cgcgacggac agcacattgg
     4981 tacgcgtgat gctgccggaa tgagtgaaga cgatattatc accatgatgg tcgggcgaga
     5041 gttaaccgcg ctttacccta atgaaccaca taccaccgga gatgaaatat tacgtattga
     5101 acatctgacg gcatggcatc cggttaatcg tcatattaaa cgagttaatg atgtctcgtt
     5161 ttccctgaaa cgtggcgaaa tactgggtat tgccggactc gttggtgccg gacgtaccga
     5221 gaccattcag tgcctgtttg gtgtgtggcc cggacaatgg gaaggaaaaa tttatattga
     5281 tggcaaacag gtagatattc gtaactgtca gcaagccatc gcccagggga ttgcgatggt
     5341 ccccgaagac agaaagcgcg acggcatcgt tccggtaatg gcggttggta aaaatattac
     5401 cctcgccgca ctcaataaat ttaccggtgg cattagccag cttgatgacg cggcagagca
     5461 aaaatgtatt ctggaatcaa tccagcaact caaagttaaa acgtcgtccc ccgaccttgc
     5521 tattggacgt ttgagcggcg gcaatcagca aaaagcgatc ctcgctcgct gtctgttact
     5581 taacccgcgc attctcattc ttgatgaacc caccaggggt atcgatattg gcgcgaaata
     5641 cgagatctac aaattaatta accaactcgt ccagcagggt attgccgtta ttgtcatctc
     5701 ttctgaatta cctgaagtgc tcggccttag cgatcgtgta ctggtgatgc atgaagggaa
     5761 actaaaagct aacctgataa atcataacct gactcaggag caggtgatgg aagccgcatt
     5821 gaggagcgaa catcatgtcg aaaagcaatc cgtctgaagt gaaattggcc gtaccgacat
     5881 ccggtggctt ctccgggctg aaatcactga atttgcaggt cttcgtgatg attgcagcta
     5941 tcatcgcaat catgctgttc tttacctgga ccaccgatgg tgcctactta agcgcccgta
     6001 acgtctccaa cctgttacgc cagaccgcga ttaccggcat cctcgcggta ggaatggtgt
     6061 tcgtcataat ttctgctgaa atcgaccttt ccgtcggctc aatgatgggg ctattaggtg
     6121 gcgtcgcggc gatttgtgac gtctggctag gctggccttt gccacttacc atcattgtga
     6181 cgttggttct gggactgctt ctcggtgcct ggaacggatg gtgggtcgcg taccgcaaag
     6241 tcccttcatt tattgtcact ctcgcgggca tgttggcatt tcgcggcata ctcattggca
     6301 tcaccaacgg cacgactgta tcccccacca gcaccgcgat gtcacaaatt gggcaaagct
     6361 atctccccgc cagcaccggc ttcatcattg gcgcgcttgg cttaatggct tttgttggtt
     6421 ggcaatggcg cggaagaatg cgccgtcagg ctttgggttt gcagtctccg gcctctaccg
     6481 cagtagtcgg tcgccaggct ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga
     6541 atgattatcg tggcgttccc actcctgttc tgctgctgac gttgctgtta ctcggcggaa
     6601 tgtttatggc aacgcggaca gcatttggac gacgcattta tgccatcggc ggcaatctgg
     6661 aagcagcacg tctctccggg attaacgttg aacgcaccaa acttgccgtg ttcgctatta
     6721 acggattaat ggtagccatc gccggattaa tcctcagttc tcgacttggc gctggttcac
     6781 cttctgcggg gaatatcgcc gagctggacg caattgcagc atgcgtgatt ggcggcacca
     6841 gtctggcagg cggcgtcggg agcgtggctg gagcggtaat gggggcattc atcatggctt
     6901 cactggataa cggtatgagt atgatggatg taccgacctt ctggcagtat atcgttaaag
     6961 gtgcgattct gttgctggca gtatggatgg actccgcaac caaacgccgt tcttgatttt
     7021 gataaaaatt ttctcaaagc cggtaacgta ttaccggttt tgagtttttg catgattcag
     7081 caggaaaaga accatgttta ctaaacgtca ccgcatcaca ttactgttca atgccaataa
     7141 agcctatgac cggcaggtag tagaaggcgt aggggaatat ttacaggcgt cacaatcgga
     7201 atgggatatt ttcattgaag aagatttccg cgcccgcatt gataaaatca aggactggtt
     7261 aggagatggc gtcattgccg acttcgacga caaacagatc gagcaagcgc tggctgatgt
     7321 cgacgtcccc attgttgggg ttggcggctc gtatcacctt gcagaaagtt acccacccgt
     7381 tcattacatt gccaccgata actatgcgct ggttgaaagc gcatttttgc atttaaaaga
     7441 gaaaggcgtt aaccgctttg ctttttatgg tcttccggaa tcaagcggca aacgttgggc
     7501 cactgaacgc gaatatgcat ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt
     7561 ttatcagggg ttagaaaccg caccagagaa ctggcaacac gcgcaaaatc ggctggcaga
     7621 ctggctacaa acgctgccac cgcaaacagg gattattgcc gttactgacg cccgagcacg
     7681 gcatattctg caagtatgtg aacatctaca cattcccgta ccggaaaaat tatgcgtgat
     7741 tggcatcgac aacgaagaac tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc
     7801 tcagggcgcg cggcaaatgg gctatcaggc ggcaaaactg ttgcatcgat tattagataa
     7861 agaagaaatg ccgctacagc ggattttggt ccctccagtt cgcgtcattg aacggcgctc
     7921 aacagattac cgttcgctga ccgatcccgc tgttattcag gccatgcatt acattcgtaa
     7981 tcacgcctgt aaagggatta aagtggatca ggtactcgat gcggtcggga tctcgcgctc
     8041 caatcttgag aagcgtttta aagaagaggt gggtgaaacc atccatgcca tgattcatgc
     8101 cgagaagctg gagaaagcgc gcagtctgct gatttcaacc accttgtcga tcaatgagat
     8161 atcgcaaatg tgcggttatc catcgctgca atatttctac tctgttttta aaaaagcata
     8221 tgacacgacg ccaaaagagt atcgcgatgt aaatagcgag gtcatgttgt agcgatgatg
     8281 agaattgtcg gcgtcgcatc aggtaactca gcacaaccac ctgatgcgat taaaagcctt
     8341 atccgaccga caagtcggat aaggctcgag gaaatacaat tacatatgcg ccgcgattaa
     8401 ccgttggtta tcctggtaca ttgcgaacag gtagttgtta taactcttcc ctttggtcga
     8461 gtagcccttc agcttgtgaa tcatcgctgt ggcagtcact tcctgatcag ctttacgcag
     8521 ctgcgcacgc gatttacgga acgaagagta agccggatgc gtattgaggt tagtgacata
     8581 ggcgctcacc gattctttga cagaactaaa ctgtgagtac cctttcactt tacctggcgc
     8641 attggtacaa cgtcctttca tgcatttcat gccgaacagg ttgttgttgt tgcgcgccag
     8701 cttcgacgtt ccccaaccgc tttctgctgc agccatcgtc gccaccatac tggtagggat
     8761 aatgtctacg cgttcaagca aggtattcca cgggattttt cgcgtattac cagaccactt
     8821 caccttgtag cgtttggcga tgtctttcag acgcgcacgc tcagcaggtg accattggcc
     8881 ctgatactgt tttgaaatca gccagttacg ttctgcagta atggctgcat tttggctggt
     8941 aatgtaaggc attacggtcc gaagaaacgc ctttttcctt ggtgttccgg aagggtattt
     9001 tcgcaaatca ggaagtgaac tactctttgc actattgcga gaatactctt gtttactgct
     9061 tacctgttta ttactagctt tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt
     9121 cttcgctaac acctcactcg aaaacaccag agtgagtaac ataagaatca tcgccccata
     9181 tcgtcgtatg ggagtcaaaa tcatcaggtc tcctggtcgg atttaatcat tccaacacct
     9241 tatatttttc acaaatttga gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag
     9301 caccccaagg aatagtccaa atctgaaact atgtcacgtg ttaacgattc agattggcgc
     9361 taaatcgcag aaaatgtggg gtttatcgca aaattcagcc gttttttgcg cgagatcgct
     9421 cacccttgct tctcatcctg tggacttacc gctcagggat gagttttgtt tggcttatcg
     9481 ctggcaaact gtctgaaatc gcagcaataa ggactcatcc gccatgaaac tcgccgcctg
     9541 ttttctgaca ctccttcctg gcttcgccgt tgccgccagc tggacttctc cggggttccc
     9601 tgcctttagt gaacagggaa cgggaacatt tgtcagccac gcgcagttgc ccaaaggtac
     9661 gcgtccacta acgctaaatt ttgaccaaca gtgctggcag cctgcggatg cgataaaact
     9721 caatcagatg ctttccctgc aaccttgtag caacacgccg cctcaatggc gattgttcag
     9781 ggacggcgaa tatacgctgc aaatagacac ccgctccggc acgccaacat tgatgatttc
     9841 catccagaac gccgccgaac cggtagcaag cctggtccgt gaatgcccga aatgggatgg
     9901 attaccgctc acgctggatg tcagcgccac ttttccggaa ggagccgccg tacgggatta
     9961 ttacagccag caaattgcga tagtgaagaa cggtcaaata acgttacaac ccgctgccac
    10021 cagcaacggt ttactcctgc tggaacgggc agaaactgac acatccgccc ctttcgactg
    10081 gcataacgcc acggtttact ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa
    10141 tgaccagagt tacggacgtc ataaagacgg tatggcggaa ataggtactt ttcacggcgg
    10201 cgatttacgc ggcctgacca acaaactgga ttatctccag cagctgggcg tcaatgcttt
    10261 gtggataagc gccccatttg agcaaattca cggctgggtc ggcggcggta caaaaggtga
    10321 tttcccgcat tatgcctacc acggttatta cacacaggac tggacgaatc ttgatgccaa
    10381 tatgggcaac gaagccgatc tacgaacgct ggttgatagc gcgcatcagc gcggtattcg
    10441 tattctcttt gatgtcgtga tgaaccacac cggctatgcc acgctggcgg atatgcagga
    10501 gtatcagttt ggcgcgttat atctttctgg tgacgagctg aaaaaaacgc tgggtgaacg
    10561 ctggagtgac tggaaacctg ccgccgggca aacctggcat agctttaacg attacattaa
    10621 tttcagcgac aaaacaggct gggataaatg gtggggaaaa aactggatca gaacggatat
    10681 cggcgattac gacaatcctg gatttgacga tctcactatg tcgctggcct ttttgccgga
    10741 tatcaaaacc gaatcaacga ccgcttctgg tctgccggtg ttctataaaa acaaaacgga
    10801 tacccacgct aaagtcatcg acggctttac ccctcgcgat tacttaaccc actggttaag
    10861 tcagtgggtc cgcgactatg ggattgatgg ttttcgagtc gataccgcca aacatgttga
    10921 gttgcccgcc tggcagcaac tgaaaaccga agccagcgcc gcgcttcgcg aatggaaaaa
    10981 agctaacccc gacaaagcat tagatgacaa acctttctgg atgaccggtg aagcctgggg
    11041 ccacggcgtg atgcaaagtg actactatcg ccacggcttc gatgcgatga tcaatttcga
    11101 ttatcaggag caggcggcga aagcagtcga ttgtctggcg cagatggata cgacctggca
    11161 gcaaatggcg gagaaattgc agggtttcaa cgtgttgagc tacctctcgt cgcatgatac
    11221 ccgcctgttc cgtgaagggg gcgacaaagc agcagagtta ttattattag cgccaggcgc
    11281 ggtacaaatc ttttatggtg atgaatcctc gcgtccgttc ggtcctacag gttctgatcc
    11341 gctgcaaggt acacgttcgg atatgaactg gcaggatgtt agcggtaaat ctgccgccag
    11401 cgtcgcgcac tggcagaaaa tcagccagtt ccgcgcccgc catcccgcaa ttggcgcggg
    11461 caaacaaacg acactttcgc tgaagcaggg ctacggcttt gttcgtgagc atggcgacga
    11521 taaagtgctg gtcatctggg ctgggcaaca gtgacttttc cggcttcccg ttcgtcagta
    11581 cctcgggaag ccgccaacca ggataaaatg tcagccctaa tcagcgttgc aggataaagc
    11641 accactcact cttcaacaga ccgatttgca ccccagcaaa tgtagcgtta ttgttacctt
    11701 ccttgctaca gagttcgaca gatatcccgc tatgacattc tccctttttg gtgacaaatt
    11761 tacccgccac tccggcatta cgctgctgat ggaagatctg aacgacggtt tacgcacgcc
    11821 tggcgcgatt atgctcggcg gcggtaatcc ggcgcagatc ccggaaatgc aggactactt
    11881 ccagacgcta ctgaccgaca tgctggaaag tggcaaagcg actgatgcac tgtgcaatta
    11941 cgacggtcca caggggaaaa cggagctact cacactgctt gccggaatgc tgcacgagaa
    12001 gttgggttgg gatatcgaac cacagaatat tgcactaaca aacggcagcc agagcgcgtt
    12061 tttctactta tttaacctgt ttgccggacg ccgtgccgat ggtcgggtca aaaaagtgct
    12121 gttcccgctg gcaccggaat acattggcta cgctgacgcc ggactggaag aagatctgtt
    12181 tgtctctgcg cgtccgaata ttgaactgct gcctgaaggc cagtttaaat accacgtcga
    12241 ttttgagcat ctgcatattg gcgaagaaac cgggatgatt tgcgtctccc ggccgacgaa
    12301 tccaacaggc aatgtgatta ctgacgaaga gttgctgaag cttgacgcgc tggcgaatca
    12361 acacggcatt ccgctggtga ttgataacgc ttatggtgtc ccgttcccgg gtatcatctt
    12421 cagtgaagcg cgcccgctat ggaatccgaa tatcgtgctg tgcatgagtc tttccaagct
    12481 gggtctacct ggctcccgct gcggcattat catcgccaat gaaaaaatca tcaccgccat
    12541 caccaatatg aacggcatta tcagcctggc acctggcggt attggtccgg cgatgatgtg
    12601 tgaaatgatt aagcgtaacg atctgctgcg cctgtctgaa acagtcatca aaccgtttta
    12661 ctaccagcgt gttcaggaaa ctatcgccat cattcgccgc tatttaccgg aaaatcgctg
    12721 cctgattcat aaaccggaag gagccatttt cctctggcta tggtttaagg atttgcccat
    12781 tacgaccgag cagctctatc agcgcctgaa agcacgcggc gtgctgatgg tgccggggca
    12841 caacttcttc ccagggctgg ataaaccgtg gccgcatacg catcaatgta tgcgcatgaa
    12901 ctacgtacca gagccggaga aaattgaggc gggggtgaag attctggcgg aagagataga
    12961 aagagcctgg gctgaaagtc actaaaaatt gcctgatgcg ctacgcttat caggcctaca
    13021 tgatttctgc aatatattga atttggaaga atttgtaggc cggataaggc gtttacgccg
    13081 catctggcat aaacaaagcg cactattccg gcgtttagaa acgccggaaa acgtttcaaa
    13141 caggctgctg ccgtagccga tgccgctgca ctttctcgac atccatgcac tgcaacgcct
    13201 ttgtcgggca ggcttcaaca caggccggtc ccgtctcccg atgccagcac agatcgcact
    13261 taatcgccct cgcctttttg cgcgaagaaa cgacctccat cgcaccaaac gggcaagcca
    13321 gcatacagct tttacagcca atgcaacgtg attgttcaac gaaaatatgc ccatgttcgc
    13381 ggcttatcgc gtcaacaggg cagacattcg cgcacggtgc atcttcacac tgatgacagg
    13441 ctactgccgt ggtccagctg tggtctttaa taacacgaat acgggaaata aactcgtctg
    13501 gtgacaacgc agcgcaatcc tgattctcct gatgcgacac tgcgcaagcc acttcacagg
    13561 tacggcaacc gatacatttc gtcgcatccg caataataaa ccggttcatg atcactcctt
    13621 tgcactaaaa cagcagagtg ccagagagca cgtccctact gctttgatcc aacgaaggaa
    13681 atgttaaagt tttggcactt gtccaaagcg ttatgtgatc gcgcccaggt catccctgac
    13741 agtaaatccc agttcattag aaatagcctg cgcggtttca cgcagtggtt tcaggagatt
    13801 tttctccccc acctgtttca gacgtgatgt cgaaagcgaa atcgacacgg cactacgaat
    13861 tgatatgttc cttgctgtaa cgccgcttcc acgctgctgg cgttaaaccg gtgtgtttgc
    13921 gaaaaatctg ccggaaatag ccaacgtcat taaacccgca caacgccgcc acttccgtga
    13981 gcgatcgcgc ttcgctaatt aacagctttt cggcttcgcg cacccgctgg cgatggattg
    14041 cttctgttaa cgtcagatga aacacccgcc ggtagacgcg cccaagataa tcggcattgc
    14101 agtacagctc gcgggccaga agcgccgtag aaagcgggcg atgatattgc gtgcggatca
    14161 gtttctgcgc tttccaggca agggtggtgc ctgcgccatc ggcctgttca tcaagacgcg
    14221 cgtcacgggt gagctgctgc aaaatgagca gcacaataaa ctccagcgcc ggagtgcgtt
    14281 gatgcttttc ctgttcgctt aagaactggc gaaacagggc caccagcgcc tgcggatcga
    14341 caaccttcgt ttgttgcggc acgctaagat gagtaagcca gcgagggtcg ttgtccggcg
    14401 ctttaacctc aaaatgcagc cagtaaaatt tgagatcggc gggaaaatcc tccaccccaa
    14461 catgttgtcg gttcggccag agtaataaac tctctcccgc atcgaccaga aatatccgtt
    14521 cttcttcctg aatcgttaat cgaccttttt cgacaaagat aatttcccat gactccagcc
    14581 gccttgcagg atgcctccct acgccacggg aaataaataa tccaccattt tgcactttaa
    14641 tcggtaacgt catggataat tcgagcattg atatgtaatt tcccgcttat tcattaatga
    14701 ttaaacacat taccttttga ttattaatca aaaacattaa tttcacctga tccatttcac
    14761 acttttaaat tcaggtcgga atcgtcacct ttttttactc ttttctcgcc ttgttgatat
    14821 tcccgttcac ggcagaatta atcaggtcat ttcaataaaa ctaaaaattg ctccctattc
    14881 acgaggaata tctatgacct ctacaccgat tactaccgct gatatcgcta aaggtaaaat
    14941 tgacgatgcg ttatctgtac gggaaaaaat aggctacggc ctgggtgacg caggcggcac
    15001 tgtaataact tgcctgatca tgaattttct cacctttttc tacaccgacg tttttggatt
    15061 aactccggcg ctggttggca cgctgtttat tgcactgcgc gtgtttgatg ccatctccga
    15121 cccggtgatg ggcgtcattg ccgaccggac gcaaagtcgc tgggggcgct ttcgtccgtg
    15181 gcagctatgg attgccattc ccatcggcat tatcggcatc ctgacgttca ccgtgccaga
    15241 tgccagcatg ggagtaaaaa tcgcctgggc gttcggtact tacctgctcc tttcagtcgg
    15301 ttataccgcc atcaacgtac cgtactgcgc gctgatcaac accatgacca cccgccacaa
    15361 tgaagtgatc tcctgccagt cctggcgatt cgttctctgc ggcgtagcgg gatttctggt
    15421 ttcggtaggc ttaccgtgga tggtagatct cttcggtcag ggcaacgctg cacgcggcta
    15481 tcaactgggc gtcggggtat tgtgcgccat tgccgtggtg atgttcctgt gctgtttctt
    15541 ctgggttcgt gaacgggtgc cgctctccac aatggggaaa tttaccctgc gcgaacatct
    15601 tgccgggctg cggaacaacg accaactgct gctgatgctg gtcatgtctt tcctgctgat
    15661 taacgtcttt aacattcgcg gcggtgggta tatgtacttc attacctacg tcttacaagg
    15721 cagtacgggc tacacgtcgc tgttcttcac catggtcacc ttcgcctcca ttatcggctc
    15781 ggtgattgtc agcccgttaa cgcggcgttt cgataccgtc aaaatttact actacaccaa
    15841 ccagctcctc gctgcgctgg cggtgttgat gtggttcctg ccctccggcc cggcttatca
    15901 aacgctgtgg ctggcggtga tcctcggtaa tggcgtaatt cttggcttca cattgccact
    15961 gcacttctca ttgatggcct ttgccgatga ctacggcgag tggaaaaccc gcgtacgttc
    16021 ttccggcatg aacttcgcct tcaatctgtt tttcatcaag ctggcctggg cctccagcgc
    16081 cgggatcatc agcctgctgt ttatttttgt cgcctaccag cctggcgtgg aaaaccagac
    16141 cgccagttcg cttggcggga tcgcggcgat ggaaacatta ctgcctgcgc tattccacct
    16201 gctgctggca ggggcgatcc gcttttgcaa actcaataat cctatgatgt cacgcatagc
    16261 taccgacctg cgtcagcgtc atgtacagcc ttaaggagca caagatgaac atttcggaag
    16321 tcgatctgca taaactgacg gtcagcgatc cgttcctcgg tcagtaccaa caactggtcc
    16381 gcgacgtggt gattccttat cagtgggatg ctttgaacga tcgtatccca gaagcggaac
    16441 ccagccatgc gattgaaaac tttcgcattg ccgccggact tcaggagggt gaattttacg
    16501 ggatggtgtt tcaggacagc gacgtcgcca aatggctgga agcggtagcc tggtcgctgt
    16561 gccagaagcc ggacgccgaa ctggaaaaaa ccgccgacga ggtaatcgaa ctgatcgcct
    16621 ccgcccaatg cgaagacggc tatctcaata cttactttac ggtaaaagca cccgaagaac
    16681 gctggagcaa tctggcggag tgtcatgaac tttactgcgc aggtcatctg attgaagccg
    16741 gagtcgcctt cttccaggcc acgggcaagc ggcgcttgct ggaagtggtt tgccgcctgg
    16801 ccgatcatat cgacagcgta tttggtccag atgaaagtaa gttacacggt tatcctggtc
    16861 acccggaaat tgaactggca ctaatgcgcc tgtatgaagt gactgaagag ccgcgctacc
    16921 tggcactgac gaactatttt gtcgaacagc gtggtgcgca accgcactat tacgaccagg
    16981 aatatgaaaa gcgcgggcag acatcgcact ggcacaccta cggcccggcg tggatggtga
    17041 aagacaaagc ctacagccag gcacatttgc cccttgcgca acagcaaacc gccatcggtc
    17101 acgcggtacg ttttgtctat ctgatgaccg gcgtcgcgca tctcgcgcgt ttaagtcacg
    17161 atgacagcaa gcgtcaggac tgcctgcggc tgtggaacaa tatggcccag cgtcagttat
    17221 atattaccgg cggcattggc tcacagagca gcggcgaagc gttcagcagc gattacgatc
    17281 tgccgaatga cacggtttac gccgaaagtt gtgcttccat cggcctgatg atgttcgccc
    17341 gacgaatgct ggaaatggaa ggcgacagtc aatatgccga tgtgatggag cgcgcgctgt
    17401 acaacactgt gctcggtggc atggcactgg atggcaaaca tttcttctat gtgaatccgc
    17461 tggaagtaca tccaaaatcg ctgaaattca accatatcta cgatcacgtt aaaccgatcc
    17521 gccagcgttg gtttggctgc gcttgttgtc cgccaaatat ctcccgcgtg ctgacctcga
    17581 ttggtcatta tctctacacg ccgcgtgaag atgcgttgta tatcaacatc tacgcaggaa
    17641 acagcatgga agtgccggta gaaaatggca cgctgcgcct gcgggttagc gggaactatc
    17701 cgtggcagga gcaggtgacg attgtggtcg aatcgcccca gccggtacgt catacgctgg
    17761 ctttacgtct gccggactgg tgcacacagc cgcagatcat attgaatggg gaagaggtcg
    17821 agcaggatat tcgtaaaggg tatttgcaca ttacccgcga atggcaggag ggcgatacgc
    17881 tgaatctgac tttgccgatg ccggtacgcc gcgtttacgg taacccgctg gtgcgtcacg
    17941 tcgccggaaa agtggcgatt cagcgcggcc cgctggtgta ttgcctggaa caggccgaca
    18001 acggcgagtc actgcataat ctgtggctgc ccaccgatgc gccatttacg acatttgaag
    18061 gcaagggatt gtttagccat aagatcttaa tccaggcacc gggttaccgg tatgaacaga
    18121 gcaatccaga gcagcaaccg ctgtggcatt acgacagcgc gccagccaaa cgccagacgc
    18181 aaactctgac ctttatcccg tggtttagct gggccaaccg gggtgaaggc gaaatgcgga
    18241 tctgggtgaa tgaggaaaag cattgccatc cgtaggttgg ataaggcact cgcgccgcat
    18301 ccaacgtaag gagcacattg cctgatgcga cgcttaacac gtcttatcag gcatacaagt
    18361 cggtgcgaga gcgtaggcca gccgcaccga catccaccgg tgaattaaaa acgccagctc
    18421 acgccggtga caaaggtaaa actctcatta cgatcaatca tcgggctgtc ggtaatctct
    18481 tccggcagct cgctgtatcc tgcgctcgcc atcaatacga cgtgctctcc tatcgggtat
    18541 tttgccgtca ggctgacata gggcacccag gcatcctgcg cggaataact tgccagaccg
    18601 ctacgacggg actcactctc tgaaatgcca tagtaatagt cactgaaatt ctcgtcataa
    18661 tagagaacgc ccagcgcagg tgtcagcgac agacgaccta tctgcatttt gtggaatacc
    18721 gatagctccc ccacccagcc gttgctgtta tccagaacgt ccgcagctgc agaggctttc
    18781 acggttcccc aacgctcgtg gtgataccag gcaacccccg ccatagccgt actatcacgc
    18841 ttatcaagct gttgcatggc ataatcgtca ttatcggcag ggtcaaattc cagcggcatc
    18901 caggatgcgg tcaggctaag ttcatttttt tcactttgcg acagaataaa acctaacgtg
    18961 gtctgacgaa cataaaaaga atcaccttca taactaatca ggggaattgc ctttgtattt
    19021 tcattataac cgcgataagg cgattcatta taagccgcac cggcaccaat ggataattca
    19081 gaagcagttg cgcttgccat aaaaggcaac gcaaataacg ccactatatt gcggttaatt
    19141 aacatgtcag gtattccatt ttaaaataag ataaaaaaga gtcggcatag taatgcttac
//
LOCUS       DAZKFU020000076        19020 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZKFU020000076 DAZKFU020000000
VERSION     DAZKFU020000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.076"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(125..1774)
                     /gene="pgi"
                     /locus_tag="ACHOQQ_RS10565"
                     /old_locus_tag="pgaptmp_002113"
     CDS             complement(125..1774)
                     /gene="pgi"
                     /locus_tag="ACHOQQ_RS10565"
                     /old_locus_tag="pgaptmp_002113"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGU4470366.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSQAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2011..2207
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2299..3648
                     /gene="lysC"
                     /locus_tag="ACHOQQ_RS10570"
                     /old_locus_tag="pgaptmp_002114"
     CDS             2299..3648
                     /gene="lysC"
                     /locus_tag="ACHOQQ_RS10570"
                     /old_locus_tag="pgaptmp_002114"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGU4470367.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLSMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3901..4173
                     /locus_tag="ACHOQQ_RS10575"
                     /old_locus_tag="pgaptmp_002115"
     CDS             3901..4173
                     /locus_tag="ACHOQQ_RS10575"
                     /old_locus_tag="pgaptmp_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGU4470368.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4174..5046)
                     /gene="rluF"
                     /locus_tag="ACHOQQ_RS10580"
                     /old_locus_tag="pgaptmp_002116"
     CDS             complement(4174..5046)
                     /gene="rluF"
                     /locus_tag="ACHOQQ_RS10580"
                     /old_locus_tag="pgaptmp_002116"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGU4470369.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKTKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5394..6341
                     /locus_tag="ACHOQQ_RS10585"
                     /old_locus_tag="pgaptmp_002117"
     CDS             5394..6341
                     /locus_tag="ACHOQQ_RS10585"
                     /old_locus_tag="pgaptmp_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGU4470370.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6411..7217
                     /locus_tag="ACHOQQ_RS10590"
                     /old_locus_tag="pgaptmp_002118"
     CDS             6411..7217
                     /locus_tag="ACHOQQ_RS10590"
                     /old_locus_tag="pgaptmp_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4470371.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7227..7634
                     /locus_tag="ACHOQQ_RS10595"
                     /old_locus_tag="pgaptmp_002119"
     CDS             7227..7634
                     /locus_tag="ACHOQQ_RS10595"
                     /old_locus_tag="pgaptmp_002119"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HGU4470372.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNTNVEAVEFVPGENAGDIV
                     AKLKKLVSIHSQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7634..8128
                     /locus_tag="ACHOQQ_RS10600"
                     /old_locus_tag="pgaptmp_002120"
     CDS             7634..8128
                     /locus_tag="ACHOQQ_RS10600"
                     /old_locus_tag="pgaptmp_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGU4470373.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8194..8991
                     /locus_tag="ACHOQQ_RS10605"
                     /old_locus_tag="pgaptmp_002121"
     CDS             8194..8991
                     /locus_tag="ACHOQQ_RS10605"
                     /old_locus_tag="pgaptmp_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGU4470374.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9002..9826
                     /locus_tag="ACHOQQ_RS10610"
                     /old_locus_tag="pgaptmp_002122"
     CDS             9002..9826
                     /locus_tag="ACHOQQ_RS10610"
                     /old_locus_tag="pgaptmp_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGU4470375.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9878..11107
                     /gene="sorE"
                     /locus_tag="ACHOQQ_RS10615"
                     /old_locus_tag="pgaptmp_002123"
     CDS             9878..11107
                     /gene="sorE"
                     /locus_tag="ACHOQQ_RS10615"
                     /old_locus_tag="pgaptmp_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HGU4470376.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSMVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSESQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11489..12178
                     /gene="pepE"
                     /locus_tag="ACHOQQ_RS10620"
                     /old_locus_tag="pgaptmp_002124"
     CDS             11489..12178
                     /gene="pepE"
                     /locus_tag="ACHOQQ_RS10620"
                     /old_locus_tag="pgaptmp_002124"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGU4470377.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12269..13900)
                     /locus_tag="ACHOQQ_RS10625"
                     /old_locus_tag="pgaptmp_002125"
     CDS             complement(12269..13900)
                     /locus_tag="ACHOQQ_RS10625"
                     /old_locus_tag="pgaptmp_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGU4470378.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14120..17803)
                     /gene="metH"
                     /locus_tag="ACHOQQ_RS10630"
                     /old_locus_tag="pgaptmp_002126"
     CDS             complement(14120..17803)
                     /gene="metH"
                     /locus_tag="ACHOQQ_RS10630"
                     /old_locus_tag="pgaptmp_002126"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGU4470379.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRVRKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLETARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18003..18827
                     /gene="iclR"
                     /locus_tag="ACHOQQ_RS10635"
                     /old_locus_tag="pgaptmp_002127"
     CDS             18003..18827
                     /gene="iclR"
                     /locus_tag="ACHOQQ_RS10635"
                     /old_locus_tag="pgaptmp_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGU4470380.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4489 a         4982 c         4988 g         4561 t
ORIGIN      
        1 tgtacatatc ggcatcgacc tgtaggcctg ataagacgcg accgcgtcgc atcaggcatc
       61 ggttgccgga tgcggcgtga acgccttatc cggcctacat agcggcatca ggcatgaacg
      121 atgattaacc gcgccacgct ttatagcggt taatcagacc attggtcgag ctatcgtggc
      181 tgctgatttc tttatcatct ttcagctctg gcagaatacg gttcgccagc tgtttaccca
      241 gttccacgcc ccactggtcg aaggtgaaga tgttcaggat cacgccctga gtaaagattt
      301 tgtgctcata cagcgcaatc aacgcaccca ggctgaacgg agtgatttca cgcagcagga
      361 tggagttggt cgggcggtta ccttcgaaca ctttgaacgg caccacgtag tcaagcgttg
      421 ccggatcttt accctgatcg cgatattcct gctcaaccac ttcgcgggat ttaccaaacg
      481 ccagcgcttc ggtctgagca aagaagttag acagcagttt ctggtgatga tcagagagcg
      541 ggttatgggt gatagccggg gcgatgaaat cgcacggtac cattttggtt ccctgatgga
      601 tcagctggta gaacgcgtgc tgaccgttag tgcctggttc accccagata atcgggccag
      661 tctggtaatc cacaacgtta ccgttacggt caacatactt accgttggac tccatattgc
      721 cctgctggaa gtacgccgca aaacggtgca tatactggtc atacggcaga atcgcttcag
      781 tttccgcacc aaagaaattg ttgtaccaga tgccaatcag cgccagcaag acaggcaggt
      841 ttttctcggc aggcgtggtg gagaaatgct tgtccatcgc gtgtgcgcca gaaagcagtt
      901 caacgaagtt atcaaagccg atggagagaa caatcgacag gccaatcgct gaccacaaag
      961 agtaacgacc gccgacccag tcccagaact cgaacatgtt ggcagtatca ataccaaact
     1021 cgccaacggc tttggcattg gtggagagcg ccgcaaagtg tttcgcaacg tgtttttcat
     1081 cacctgccgc tttcaggaac cagtcacgcg cgctatgggc gttggtcatg gtttcctgag
     1141 tggtgaaggt tttagatgct accaggaaca gggtagtttc cgggtttact tttttcagca
     1201 cttccgcgat gtgggtgcca tcgacgttag acacaaagtg catgttcagg tggtttttgt
     1261 acggacgcag tgcttcggtc accatgtacg ggccgaggtc agaaccgccg atcccgatgt
     1321 tcactacgtc agtgattgct ttgccggtat aacccttcca ctcaccggag ataatcgctt
     1381 gtgagaaggt tttcatcttc tccagcaccg cgttgacttc cggcattacg tctttgccat
     1441 caaccagaat cggggtattg ctacggttac gcagcgctac gtgcagcacg gcgcggtttt
     1501 cagtgcggtt gatcttctcg ccagagaaca tcgacttaat cgcgcccgcc agatcgcact
     1561 ctttcgccaa atcttgtaat ttcgccagcg tctcttcagt gatgcggttt ttggagtaat
     1621 ccaccagcat ctgatcgtcg aaggttgcgg agaacttaga aaaacgatcg ccgtctttag
     1681 caaaaagatc ggcgatcgta acgtctttca tttcatcgaa gtgtttctgt agtgcctgcc
     1741 aggcagcggt ctgcgttgga ttgatgtttt tcattagcaa tactcttctg attttgagaa
     1801 ttgtgacttt ggaagattgt agcgccagtc acagaaaaat gtgatggttt tagtgccgtt
     1861 agcgtaatgt tgagtgtaaa cccttagcgc agtgaagcat ttattagctg aactactgac
     1921 cgccaggagt ggatgaaaaa tccgcatgac cccgtcgttg acaaccgccc cgctcaccct
     1981 ttatttataa atgtactacc tgcgctagcg caggccagaa gaggcgcgtt gcccaagtaa
     2041 cggtgttgga ggagccagtc ctgtgataac acctgagggg gtgcatcgcc gaggtgattg
     2101 aacggctggc cacgttcatc atcggctaca ggggctgaat cccctgggtt gtcaccagaa
     2161 gcgttcgcag tcgggcgttt cgcaagtggt ggagcacttc tgggtgaaaa tagtagcgaa
     2221 gtatcgctct gcgcccaccc gtcttccgct cttcccttgt gccaaggctg aaaatggatc
     2281 ccctgacacg aggtagttat gtctgaaatt gttgtctcca aatttggcgg taccagcgta
     2341 gctgattttg acgccatgaa ccgcagcgct gatattgtgc tttctgatgc caacgtacgt
     2401 ttagttgtcc tctcggcttc tgctggtatc actaatctgc tggtcgcttt agctgaagga
     2461 ctggaacctg gcgagcgatt cgaaaaactc gacgctatcc gcaacatcca gtttgccatt
     2521 ctggaacgtc tgcgttaccc gaacgttatc cgtgaagaga ttgaacgtct gctggagaac
     2581 attactgttc tggcagaagc ggcggcgctg gcaacgtctc cggcgctgac agatgagctg
     2641 gtcagccacg gcgagctgat gtcgaccctg ctgtttgttg agatcctgcg cgaacgcgat
     2701 gttcaggcac agtggtttga tgtacgtaaa gtgatgcgta ccaacgaccg atttggtcgt
     2761 gcagagccag atgtagccgc gctggcggaa ctggccgcgc tgcagctgct cccacgtctc
     2821 aatgaaggct tagtgatcac ccagggattt atcggtagcg aaaataaagg tcgtacaacg
     2881 acgcttggcc gtggaggcag cgattatacg gcagccttgc tggcggaggc tttacacgca
     2941 tctcgtgttg atatctggac cgacgtcccg ggcatctaca ccaccgatcc acgcgtagtt
     3001 tccgcagcaa aacgcattga tgaaatcgcg tttgccgaag cggcagagat ggcaactttt
     3061 ggtgcaaaag tactgcatcc ggcaacgttg ctacccgcag tacgcagcga tatcccggtc
     3121 tttgtcggct ccagcaaaga cccacgcgca ggtggtacgc tggtgtgcaa taaaactgaa
     3181 aatccgccgc tgttccgcgc gctggcgctt cgtcgcaatc agactctgct cactttgcac
     3241 agcctgagta tgctgcattc tcgcggtttc ctcgcggaag ttttcggcat cctcgcgcgg
     3301 cataatattt cggtagactt aatcaccacg tcagaagtga gcgtggcatt aacccttgat
     3361 accaccggtt caacctccac tggcgatacg ttgctgacgc aatctctgct gatggagctt
     3421 tccgcactgt gccgggtgga ggtggaagaa ggtctggcgc tggtcgcgtt gattggcaat
     3481 gacctgtcaa aagcctgcgg cgttggcaaa gaggtattcg gcgtactgga accgttcaac
     3541 attcgcatga tttgttatgg cgcatccagc cataacctgt gcttcctggt gcccggcgaa
     3601 gatgccgagc aggtggtgca gaagctgcat tttaatttgt ttgagtaaat actgtatggc
     3661 ctggaagcta tatttcgggc cgtattgatt ttcttgtcac tatgctcatc aataaacgag
     3721 cctgtactct gttaaccagc gtctttatcg gagaataatt gcctttaatt tttttatctg
     3781 catctctaat taattatcga aagagataaa tagttaagag aaggcaaaat gaatattatc
     3841 agttctgctc gcaaacgaat tcccgtacac tgaactctct ggctcactga gggcaccatc
     3901 atggcactcc cccgtattac ccaaaaagag atgactgaac gcgagcagcg tgaacttaaa
     3961 acactgctgg atcgggcgcg gattgcgcat ggtcgcgtac tgaccaactc cgaaaccaac
     4021 agcattaaga aagagtacat cgataaatta atggtcgagc gtgaggctga agcgaaaaaa
     4081 gcccgccagt taaagaaaaa gcaggcttat aaaccggatc ctgaagcgtc atttagctgg
     4141 tcagcaaata cgtcgacgcg cggcaggcgc tgattagcgc cccttctttt tacgccccgg
     4201 cgaggtaaaa cgcttaccgt tggacgccgg gcgaccgcct ttttccgccg ttttttccat
     4261 cttaacgact ggacgtttga tgcccgccgt tttcggtttg gccttcgttt taggttttgc
     4321 ctcggaagag gaattttcga tgagcttaaa gagatcgata agctcgtcgt cggttaaatc
     4381 gcgccattct cccagcggaa tgccgcttaa gctgacgttc ataatgcgcg tacgttccag
     4441 ctttttcact tcatagccga aatgctcgca catgcgacgg atctgacggt tcagcccctg
     4501 caccagggta atgcggaaga caaacggcgc ttcttttttg actttacact ttttagtcac
     4561 tgtcccgagg attggcaccc ccgcccccat accacgaata aattcatcgg taatcggttt
     4621 atcgaccgtc accagatact ctttctcatg atcgttaccc gcacggagga tcttattcac
     4681 cagatcgccg tgattggtga ggaaaatcag cccctgggag tctttatcca ggcggccaat
     4741 cgggaacacg cgtttgctgt ggttaacgaa atcgacaatg ttatcgcgct cgccatcttc
     4801 ggtggtgctg acaatgccta cgggcttgtt cagggcgata agtaccaaat cttcggcttc
     4861 ccgaggctca atcaactgac cgtttacttt cacaacgtcg ccgggtttca cctgatcgcc
     4921 aatggtggct cgcttgccat taaggaacac attgccttgc tcgatatagc gatccgcttc
     4981 gcggcgtgag caaattccgc tttcgctgat gtatttattt aaacggactg atgagtcggg
     5041 cagcataaat tctcctgtaa aacgcggaat ataccgtacc tcttagcggg gaaaaacagg
     5101 gttatgcggc aacgtcgaaa ttaaatgtga tctgccacgc cttttatcac aaaagtgtct
     5161 ccggaagtac aatgcaaatt tgcgtggcaa aataaggata atcaacctat tacgaatctc
     5221 cgcacacctg tgctgataac tactacgcag agaaacaaat gtgcaagaga aagcgctatt
     5281 tctccccatg caaagagtga aaaatcgctt tgaaaggcca tcatgatggg gttatatcag
     5341 atcaagcggt gagaaagcgt atttgcacaa atgtgcagaa ccaggaggcg gcaatggaaa
     5401 acagtgacga tatccgtttg attgtgaaga ttgcccaact ctattacgaa caggatatga
     5461 cgcaggcgca aatcgcgcgc gaactgggta tttaccgcac caccatcagc cgcttgctta
     5521 aacgaggccg cgatcaggga attgtcacca tcgccatcaa ctatgactac aacgaaaatc
     5581 tctggctgga gcagcaactg aagcaaaagt ttggcctgaa agacgttgtg gtggtgtcgg
     5641 gaaatgatga ggatgaagag actcaactgg cgatgatggg gttacacggc gcgcaactgc
     5701 tggatcgctt gctggagcct ggcgatattg tcggtttttc ctggggtcgc gcggtgagcg
     5761 cactggttga aaacttgccg caggcggggc aatcgcggca gttaatctgc gtaccaatta
     5821 ttggcggacc gtccggtaaa ctcgaaagcc gctatcacgt aaacacatta acctacagcg
     5881 cggctgcgaa gctgaaaggg gaatcgcatc tcgcggattt tccggctctg ctggataacc
     5941 ccttaattcg taatgggatc atgcagtctc agcactttaa aaccatctct gcctactggg
     6001 ataatctgga tgtcgccctg gtgggaattg gctcaccggc cattcgcgac ggcgctaact
     6061 ggcatgcgtt ttatggtggt gaagagagtg acgacctgaa tgcccgccag gttgctggcg
     6121 atatttgctc gcgctttttt gatattcacg gcgcaatggt tgaaacgaat atgagcgaaa
     6181 aaacactctc tatcgaaatg aataaattaa agcaggcacg atattccatt ggcattgcca
     6241 tgagtgaaga aaaatacagc ggaattattg gtgcactgcg tggaaaatat attaattgtc
     6301 tggtaacgaa tagcagcaca gctgaactat tactgaaata aaaatcttcc cgatgtttat
     6361 cgtaataaaa attacggcgg gtccctatta tctaaaatca ggagtctgtt atgcaaacgt
     6421 ggttaaattt gcaggataaa atcattatcg tcaccggcgg cgcatccggt attggtctgg
     6481 cgattgtaga ggaattatta gcacaaggcg caaacgtaca gatggtagat attcacggtg
     6541 gcgatggtca atatgaagtt cataaaggct atcagttttg gccgaccgat atttccagcg
     6601 ccaaagaggt aaatcatacg gtagcggaaa ttatccagcg ttttggtcgc atcgacggtc
     6661 tggtcaataa cgccggggtc aatttcccgc gtctgctggt cgatgagaaa gcgcctgccg
     6721 ggcagtatga actcaacgaa gctgcattcg aaaaaatggt caatatcaac cagaaaggcg
     6781 tttttctgat gtcgcaggcg gtggcgcgac agatggtcaa acaacatgat ggcgtgattg
     6841 tgaatgtttc ctcagaaagt ggactggaag gctcagaagg ccaaagctgt tacgccgcta
     6901 ccaaagccgc gctcaatagc ttcacgcgct cctggtcgaa agagctgggt aaacacggta
     6961 tccgtgtggt cggtatcgcg cccgggattc tggaaaaaac cgggctacgc acgccggaat
     7021 atgaagaggc gctggcgtgg acgcgcaata tcaccgtcga gcagctgcgt gaaggctata
     7081 ccaaaaacgc cattcctatt gggcgcgccg gaagattagc agaagtcgct gattttgttt
     7141 gttatctgtt gtctgaacgc gccagctaca tcaccggagt aaccactaac attgcaggcg
     7201 gcaaaacgcg cggataagga ggctttatgg tcaacgccat tttttgtgcc cacggcaaat
     7261 tagcttgcgc catgctggaa tcggttcaga tggtttacgg caacaccaat gttgaagccg
     7321 ttgaatttgt gcccggtgaa aacgccggag acatcgtagc aaagctgaaa aagttagtaa
     7381 gcattcacag tcaagatgag tggctgattg ccgtggattt acagtgcggc agcccgtgga
     7441 atgcggcagc gatgcttgcg atgcgcaatc ctcgtctgcg ggtaatcagc ggactttctc
     7501 tgccgctggc gctcgaactg gttgataacc aggacagcat gaatgttgat gaactgtgtg
     7561 aacacctgac gcaaatcgca aagcaaacct gcgttgtctg gaaacaactg gcaacgacag
     7621 aggaggattt ctgatgaaca ttacgctcgc ccgcattgat gaccgcctga tccacggtca
     7681 ggtcactacc gtctggtcga aagtggctaa cgcccagcgc attattatct gcaatgacga
     7741 agtttataac gatgaagttc gccggacatt attgcgtcag gctgcgccac cgggaatgaa
     7801 ggttaacgtg gtcaatattg aaaaagccgt cgcggtttat cacaacccgc aatatcagga
     7861 cgaaaccgtt ttttatcttt tcactcgccc acaggatgcc ttagcgatgg ttcgccaggg
     7921 ggtaaaaata ggcaccctaa atattggcgg catggcctgg cggcccggta aaaaacagtt
     7981 aaccaaagcg gtttcattag atgatgatga tattaacgca tttcatgagt taaataatct
     8041 tggcgtcata ctggatttac gcgtcgtagc gtcagatccc tcaatcaata ttatcgacaa
     8101 aataaacgaa cagttaatcg caaattaaat aatagcgcct gtgtacatat gctttaacag
     8161 gcagggatga cgacacttaa aaggtgacaa atcatggaaa taagtaccct acaaattatt
     8221 gctatatttc ttttttcctg tattgccgga atgggcagcg tgctggatga attccagact
     8281 catcgcccgt taattgcctg tacggtgatt ggtttaattc tcggtgattt aaaaaccgga
     8341 ataatactcg gtggtacgct ggaattgata gctctcggct ggatgaacgt cggcgcggcg
     8401 caatctccgg attctgcact cgccagcata atctccgcca ttctggttat cgttggtcag
     8461 caaagcatcg ccaccggaat cgccatcgcg ttgcctgtgg ctgcggcagg ccaggtgctg
     8521 acggtgtttg cacgtaccat caccgtagtg ttccagcacg cggcggataa agcagcggaa
     8581 gaagcgcggt ttcgcactct cgatattctg catgtctccg cgcttggcgt gcaggcgctg
     8641 cgcgtcgcca ttccggcact gattgtctcg ctgttcgtca gcgccgatat ggtcagcaat
     8701 atgttaaacg ccattcccga atttgtgacc cgaggactac agattgctgg cggttttatc
     8761 gtggtggtcg gttacgccat ggtgctacgt atgatgggcg tgaaatattt gatgcctttc
     8821 tttttcctcg gtttcctcgc aggtggctac ctcgatctca gtctgctggc gttcggtggc
     8881 gtcggcgtga tcatggccct ggtctacatc cagctaaatc cacagtggcg taaagctgaa
     8941 cctcaactcc aggccaccgc ctccaccgcc cttgaccaac ttgacgatta acggagccat
     9001 catggaacag agaaaaatta cacgcagcga tctggtgagc atgtttctgc gctccaacct
     9061 gcaacaggcg tcctttaact ttgaacgtat tcacgggctg ggtttttgct acgacatgat
     9121 ccccgccatc aagcgacttt acccattaaa agaggatcag gttgcggcgc tcaggcgaca
     9181 cctggtgttc ttcaatacca cgccagccgt atgtggcccg gtcatcggcg tcactgccgc
     9241 catggaagag gcgcgggcca acggcgcgga aattgatgac ggtaccatta acggcatcaa
     9301 agtcggtctg atgggaccat tggcaggggt tggcgatcca ctggtctggg gaacgctgcg
     9361 cccgattacc gccgcgctcg gcgcatctct ggcactttcg ggcaacattc tcggcccgct
     9421 gctgttcttc tttattttca acgcggtgcg tctggcgatg aagtggtatg gcctacagct
     9481 cggctttcgc aaaggggtga atatcgtcag cgatatgggc gggaatgtgc tgcaaaaact
     9541 caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc gtgctggtca ccaaatggac
     9601 gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc gccgatggct ccaccgtcac
     9661 catgaccgtg cagaacattc tcgaccaact ttgccctggt ttgctggcgc tcggtctgac
     9721 gctactaatg gttcgtctgc tcaacaaaaa aattaacccg gtatggctga ttttcgccct
     9781 gtttggctta gggattatcg gcaatgcgct gggcttcctg tcctgattct tcgccccggc
     9841 acgactgccg gggccatcgc tcaacatgag gtggtttatg aaaacaacag ctctgcgtct
     9901 ttatggtaaa cgtgatttac gcctggaaac ctttgacctt cctgaaatgc aggaggatga
     9961 aatcctcgcg acggtggtca ctgacagcct gtgcctctct tcctggaaag aggccaacct
    10021 gggtgaaaac cataaaaaag tacccgacga tgtggcgacc aacccaatca tcatcggcca
    10081 cgagttttgc ggcgatattc tggccgtggg taaaaagtgg cagcacaaat tccagccggg
    10141 tcagcgttat gtgattcagg ccaacctgca actccccgac cgcccggact gccccggcta
    10201 ctccttcccg tgggtaggcg gcgaagccac gcatgtggtt attcccaacg aggtcatgga
    10261 acaagattgc ctgctggcat acgacggcga aacctatttt gaaggctcga tggttgaacc
    10321 gctttcctgc gtgattggcg cgttcaacgc caactatcat cttcaggaag gtagttataa
    10381 ccacacgatg gggattcgcc cgcaagggcg cacgctgatc ctcggcggca ccggaccaat
    10441 gggactgttg gcgattgatt atgcgctgca tggacccgtt aacccgtcgc tgctcgtcat
    10501 taccgatacc gacaacgata aattgagtta tgcgcgcaag cactatccat cagaatcgca
    10561 aacactgatt cattatctca atgccaccga tgcagcattt gatacgctaa tggcgctgag
    10621 tggcggtcac ggcttcgatg atattttcgt ctttgtgcct aatgaaggac tggtgactct
    10681 cgcctcttcc ttgctggcga cagatggttg cctgaatttc ttcgccggac cgcaggataa
    10741 acatttcagc gcgccaatta atttctacga tgtgcattat gcatttaccc actacgtggg
    10801 cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc aaactgatcg aagagaaaaa
    10861 agtgcaggcc gcaaaagtgg taacacatat tcttgggctg aatgccgcgg gcgaaaccac
    10921 gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg tataccggga aatacctgcc
    10981 gctgacgtca ctcacgcaga ttcaggatca agcactggcg gcgattctgg cgcgtcatca
    11041 ggggatctgg tccggtgagg cggagcaata tctgctcact catgcagagg caatttccca
    11101 tgattaatcg cgttactcaa ctctgcatgt cgctggctgg tcgcaccagc aacttcgcga
    11161 ccggatgtgc tgctgattgc tgaagcagca caatttgcac gagaagcata aaaaatatca
    11221 gcgccttaag gggcgctcaa attggtggca aagtgcacat tgtttatgcc ggatgcggaa
    11281 tgaagcctta ttcggtctac ataattttga aaattcaata tattgcagtt atctggtagg
    11341 ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca tcagtctcag cgcccggtgc
    11401 aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat tcgcagtggt agcattcctt
    11461 ccgtgatttc ttactaaccg gagaagtaat ggaactgctt ttattgagta actcgacgct
    11521 gccgggtaaa gcctggctgg aacatgcact gccgctaatt gctgaacagt tgcagggtcg
    11581 ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag acctgggatg attacacagc
    11641 gaaaacggct gcggttcttg ctccgctggg tgtttctgtc accggtattc atagcgttgt
    11701 cgatcccgtt gccgcgattg aaaatgctga gatcgtgatt gtcggcggcg ggaatacttt
    11761 ccagttgctg aaacagtgcc gcgagcgcgg gctgctggca ccaattactg acgtggttaa
    11821 acgtggcgca ctgtatattg gttggagcgc aggcgctaac cttgcttgcc caaccattcg
    11881 taccaccaac gatatgccga ttgtcgatcc gcaaggtttc gatgcgctga atctgttccc
    11941 gctgcaaatc aacccgcact tcaccaacgc gctgccggaa ggccataaag gtgaaacccg
    12001 tgaacagcgt attcgcgaac tgctggtcgt cgcgccagaa ctcacaatta ttggtctacc
    12061 ggaaggtaac tggatcacag tgagtaaagg tcacgcaacg ctgggtggcc cgaacaccac
    12121 ttatgtgttt aaggctggtg aagaagcggt tccgctagaa gctggtcacc gtttttaagt
    12181 gaattgccgg atgcatgatc cggcatcttg ttcatagtgc ctgatgcgac gcttgacgcg
    12241 tcttatcagg cctacaggat tcacaatatt aatactcgtc ccgtccttca tcttcatccg
    12301 gctgttccag cacactgtaa gccaccgaac aaaacagcga gttcaggcgc tgcatatctc
    12361 ccagtaagcc taaatgtagc gaactggttt caatgctctg cacgttttgc tgatgcaggc
    12421 gatcgacgtg ggcgtgggaa tagcggcgat taagaatacg aaagcgatgc ttgctgcggc
    12481 gcaaacgacg ggcgctggta acatcaccag agaagaaaac cgacattgcc agttttaaat
    12541 tactgagcaa ttgctcatag agcgcatcca gttccttcaa gccatcaagc gaaaatgccc
    12601 gccgtgctgc cagcgattta tcggcaattt cgctccccat gcgctcgacg atatcggagg
    12661 cctgttcaag gttgagcgac atttcgatga tctccgccca gcggcgcgac tcttcttctg
    12721 ccagttcctc ttttggcatc cgcgccagat acagcttaat ggcggtatag agaacgttga
    12781 tatcatcagc cagcttacgc agctctttct cctgccgtgg ctcgccgtgc atcactttat
    12841 tcagcccttc catcatctgt tccatggcgt cgccaatacg cagggtttcg cgcgcggcgt
    12901 tcgccagagc aagcgtgggc gtatccagcg cgctgacatc cagatgtttg ggtcgtagct
    12961 gggtatccag ttccggttca tcgcgaataa tcgttttgca aaaccgtgcc atcgggtcaa
    13021 caaatggcag catggccagg caacgtacaa ggttgtagaa gacgtggaaa tagatcacca
    13081 gttccgcttt tggcaatgac agcttcccca ttgtctctgc cagcaaatgg acaaacggca
    13141 ggataatcag gctacccacc agcttaaaca gcagactacc cagcgcgaca cggcgggctg
    13201 cggcattggc ggcactgttg ttgagcatcg ccagcaggcc ggaaccgagg ttagcaccaa
    13261 tcaccagaca gagcgccacg gggaaggaga taatgcctgc ggcggtcaga gtcgcagtca
    13321 gcagtacagc agcaaggctg gagtagctga taatggcgaa catcgcgcca atcagcgcat
    13381 ccagcagaat atcgccggtc agcgaggcaa agatcacctg aacgccgttt gcctgggtga
    13441 tcggcgttac ggcctgcaca atcaactcca gcgccagcaa aatcagccca agaccaatac
    13501 cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc gaggaaaaaa atcacgccga
    13561 taaaaataag taacggtgac agccaggata agtcgaaggt gagaatacgc gccattagcg
    13621 ccgtcccgac atctgcaccc agcacaatca ccagagccgg tgcgagggct accagatcct
    13681 gagcgacgaa cgaggtcacc agcatggtgg tggcattact gctctgcacc agtgcggtaa
    13741 cgccgatccc cgcgcaaaag gcgagcggct tcttttcgac gctccggcta aggacagtac
    13801 gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac aatatgagtc ccccagacca
    13861 gcagggcgac ggcagaaagc aggtgaagca gcgttaacac gagttcaggt tctccttatc
    13921 gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg aaacacgctg actggcatct
    13981 ttactgtgcc ttttgacatt cgtttttccg gctaagatta ttccagcctg tttccagtat
    14041 aagtgtttca tattaataaa gagacagggc actcattgag cgccctgttt gttgtaagga
    14101 aaagtgacag atttgtgaat cagtccgcgt cataccccag attcggtgcc agccagcgct
    14161 caacatcgga gacgctcata cctttacggc gggcatagtc ttcaacctga tcgcgctgaa
    14221 tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa ataccaaccc gaaaccgatg
    14281 caccgggcca catggcgaaa gattctgtga gtttcatgcc ggtatgtttt tccacttcca
    14341 gcagctccca gatggtggct ttttccgtat gttccgggca ggccgggtag cccggtgccg
    14401 gacggatgcc ctggtagttt tcgcggatca gttcttcgtt gctgaggttc tcgttcggcg
    14461 catagcccca gtagacttta cgcacacgct catggagata ctccgcaaag gcttcggcta
    14521 aacggtcggc aagcgctttc accatgattt tgttgtaatc atcgtgctgc gcttcaaagg
    14581 catcagccag tgcgtcctct tccagcccgc cagtcacggc aaatgcgccg atgtaatctg
    14641 ctttaccaga aagcttcggc gcaacgaagt cagcgagaca gtagttagcg aatcctgttt
    14701 tttcggtctg ttgacgcaga tggtggctga cgttgatcac atgggtgcgc gtttcgtcac
    14761 ggtagatttc aatgtcatcg cccacacggt ttgccgggaa cagccccacc acaccacgcg
    14821 gattcagcgt tttctcggcg cttaatttat ccagcatgtc gttggcgtct ttaaacagcc
    14881 gctgcgcctc aacgcctacc acttcatctt ccagaatgcg cggatacttc ccggccagcg
    14941 accaggtcat aaagaacggc gtccagtcga tgtaattacg cagcgtttcg atgctggctt
    15001 caacttcctg cacaccgaga cggtgcgcca ccggcggcgt gtaagcctgc cagtcaaaag
    15061 cgaaatcgtt atcgcgcgcc gtttccagcg tgaccggtgg tgtgcgtggt ttcttgcgcc
    15121 cgtgctgaat acgcacggtt tcgtactcct tgcgggtacg agcgacaaaa tcatcgcgct
    15181 gagtatcgga aagcagcgcc gccaccacgc caacggtgcg cgaggcattc tgcacataca
    15241 ccgtcgggcc gctgtagttc tgctcaattt tcaccgccgt gtgcgctttt gaggtggtcg
    15301 cgccgccaat caatagcgga atggtgaagc cctgacgctc catctctttc gccacgttaa
    15361 ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc aatcaaatca gcattcactt
    15421 ctttagcagt acggagaatt ttttccgcag gcaccatcac gccgagatcg acaatttcgt
    15481 agttgttaca ttgcagcacc acgccaacga tatttttacc gatatcgtgg acgtcgccct
    15541 tcacggtggc gatcaccatc ttgccgttgg ttttgccctg ctctttgctg gcttcaataa
    15601 acggttcgag atacgccacc gcctgtttca tgacgcgcgc cgatttgacc acctgtggca
    15661 ggaacatttt cccttcgcca aacagatcgc cgaccacgtt catgccgtcc atcaacgggc
    15721 cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc ttcttcggta tcctgctcga
    15781 taaactcagt aatgcctttg accagcgagt attccagacg tttattcact tcccacgagc
    15841 gccactccgc ctgctgggcg ttagcggtgt cgtcggtttt gctgccgcga tatttctcgg
    15901 caagctccag taaacgctca gtgccatcat cgcgacgatt aagaatcaca tcttccaccg
    15961 cgtcgcgcag ttcagcgggc aggtcgtcgt aaatcgccag ttgcccggcg ttgacgatcc
    16021 ccatatccat gccattgcga atagcgtagt agaggaacac cgcgtgaata gcttcgcgca
    16081 ccggatcgtt accacggaac gagaacgaga cgttggagac gccgccggaa atcagcgcgt
    16141 gcggcagttc gcgtttgatg tcttcacacg cgccgataaa gtcctgcgca tagttgttgt
    16201 gctcttcaat gccggtggcg accgcgaaga tgttcgggtc gaagatgata tcttctggcg
    16261 ggaagccaac ctcttcggtg aggattttgt acgcccgacg gcaaatctcg attttccgta
    16321 cgcgggtatc ggcctgtccc tgttcgtcaa aggccattac caccacggcc gcaccgtaac
    16381 gacgcaacaa tttcgcgtga tggataaagg catcgacgcc ctctttcatc gagatagagt
    16441 taacaatgcc tttgccctgg atacacttca gacctttttc aatgacatcc cattttgagg
    16501 agtcgatcat aatcggcacg cgagcgatat ccggttcacc ggcaatcaga ttgagaaaac
    16561 gcaccatcgc cgcttcggca tcgagcatcc cttcatccat gttgatatcg ataatctgcg
    16621 caccgttttc cacctgctgg cgggccacat ccagcgcctc gctgtatttc tcttctttga
    16681 tcaggcgctt gaacttagcg gaaccggtga cgttagtccg ctcgccgacg ttcacaaaca
    16741 ggctgtcgtc gccaatgttc agcggctcca ggccggacaa acggcaggct acgggaattt
    16801 caggcagttt gcgcggcgct aatccttcta ctgcacgact catcgctgca atatgttgtg
    16861 gcgtggtgcc acagcagccg ccgacgatat tgagaaaacc cgcttgcgcc cattcacgta
    16921 tctgttttgc catcgtgtcg gcgtcgagat cgtactcacc aaaggcgttg ggtagcccgg
    16981 cgttcgggtg cgcggtgacg tagcattccg caatccgtga cagctcctgc acgtactggc
    17041 gcagttcatc tggccccagc gcacagttca ggccaaaggt cagcgcttcg gcgtggcgca
    17101 atgagttgta aaatgcttcg gtggtctgcc cggagagcgt gcgcccggag gcgtcggtga
    17161 tggtgccgga gatcataatc ggcagctcaa cgcccagcgc ttcaaactcc gttttcaccg
    17221 caaaaaccgc cgctttggcg ttaagggtgt cgaaaacggt ttcaatcagg atcagatccg
    17281 caccaccttc caccagcgct ttggtggatt cgcgataggc cgccaccagc tggtcaaaag
    17341 tgatgttgcg gaatgccgga tcgttgacgt ccggagaaat agacgccgtg cggttggtcg
    17401 ggccgagaac accggcaacg tagcgcggtt tctctggcgt gcgcgcggtc cactcatcag
    17461 cacaggcgcg cgccagtttc gccgccgcaa agttgatttc cgccgacagg gattccatct
    17521 ggtaatccgc catcgcaatg gtcgtggagt tgaaggtgtt ggtttcgatg atatccgcgc
    17581 ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc cggtttgctt agcaccagca
    17641 gatcgttatt gcctttgagg tcgcatggcc agtcggcaaa gcgttcacca cgaaaatcgg
    17701 cttcgttcag tcgatagctc tggatcatgg tgcccatacc gccgtccagc accagaatac
    17761 gttcatttaa ctgcgcacgc agttgttcca ctttgctgct cacacttgct cccgacacgc
    17821 tcaacccaga tttaataaaa attcaacaaa ccatactggc ataaacgcat ctgtggtaaa
    17881 agcgaccacc acgcaacatg agatttgttc aacattaact catcggatca gttcagcaac
    17941 tattgcatta gctaacaata aaaatgaaaa tgatttccac gatacagaaa aaggagactg
    18001 tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc cgccgttgcc accgcaccag
    18061 cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt actggagtgg attgccgaat
    18121 ccaatggcag tgtggcactc acggagctgg cgcaacaagc cgggttaccc aattccacga
    18181 cccaccgcct gctaaccacg atgcaacagc agggtttcgt gcgtcaggtt ggcgaactgg
    18241 gacactgggc aatcggcgca catgccttta tggtcggcag cagctttctc cagagccgta
    18301 atttgttagc aattgttcac cctatcctgc gcaatctaat ggaagagtct ggcgaaacgg
    18361 tcaatatggc ggtgcttgat caaagcgatc acgaagcgat tattatcgac caggtacagt
    18421 gtacccacct gatgcgaatg tccgcgccta tcggcggtaa attacctatg cacgcttccg
    18481 gtgcgggtaa agccttttta gcccaactga gcgaagaaca ggtgacgaag ctgctgcacc
    18541 gcaaagggtt acatgcctat acccacgcaa cgctggtgtc tcctgtgcat ttaaaagaag
    18601 atctcgccca aacgcgcaaa cggggttatt catttgacga tgaggaacat gcgctggggc
    18661 tacgttgcct tgcagcgtgt attttcgatg agcaccgtga accgtttgcc gcaatttcta
    18721 tttccggacc gatttcacgt attaccgatg accgcgtgac cgagtttggc gcgatggtga
    18781 ttaaagcggc gaaggaagtg acgctggcgt acggtggaat gcgctgactt tttctggcgg
    18841 gcagaggcaa tattctgccc gtcatacctg agtggtaata gaataagggt gtctgttaat
    18901 cgcattgacg ccaaaataac ttattgtcat acacttcaca cacttttatt aactcagcat
    18961 tatttttaaa catcaaacca cttaattatt aacaaaatcg caagatctca ttccaccgaa
//
LOCUS       DAZKFU020000077        18878 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZKFU020000077 DAZKFU020000000
VERSION     DAZKFU020000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.077"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..624
                     /locus_tag="ACHOQQ_RS10640"
                     /old_locus_tag="pgaptmp_002128"
     CDS             <1..624
                     /locus_tag="ACHOQQ_RS10640"
                     /old_locus_tag="pgaptmp_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU4470381.1"
                     /translation="MLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTG
                     NNTNCNSVRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQ
                     GEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQY
                     GKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(662..1561)
                     /gene="nhaR"
                     /locus_tag="ACHOQQ_RS10645"
                     /old_locus_tag="pgaptmp_002129"
     CDS             complement(662..1561)
                     /gene="nhaR"
                     /locus_tag="ACHOQQ_RS10645"
                     /old_locus_tag="pgaptmp_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGU4470382.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1627..2793)
                     /gene="nhaA"
                     /locus_tag="ACHOQQ_RS10650"
                     /old_locus_tag="pgaptmp_002130"
     CDS             complement(1627..2793)
                     /gene="nhaA"
                     /locus_tag="ACHOQQ_RS10650"
                     /old_locus_tag="pgaptmp_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGU4470383.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3379..3531
                     /gene="mokC"
                     /locus_tag="ACHOQQ_RS10655"
                     /old_locus_tag="pgaptmp_002131"
     CDS             3379..3531
                     /gene="mokC"
                     /locus_tag="ACHOQQ_RS10655"
                     /old_locus_tag="pgaptmp_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGU4470384.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3635..4765)
                     /gene="dnaJ"
                     /locus_tag="ACHOQQ_RS10660"
                     /old_locus_tag="pgaptmp_002132"
     CDS             complement(3635..4765)
                     /gene="dnaJ"
                     /locus_tag="ACHOQQ_RS10660"
                     /old_locus_tag="pgaptmp_002132"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGU4470385.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4854..6770)
                     /gene="dnaK"
                     /locus_tag="ACHOQQ_RS10665"
                     /old_locus_tag="pgaptmp_002133"
     CDS             complement(4854..6770)
                     /gene="dnaK"
                     /locus_tag="ACHOQQ_RS10665"
                     /old_locus_tag="pgaptmp_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGU4470386.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            7147..7551
                     /gene="yaaI"
                     /locus_tag="ACHOQQ_RS10670"
                     /old_locus_tag="pgaptmp_002134"
     CDS             7147..7551
                     /gene="yaaI"
                     /locus_tag="ACHOQQ_RS10670"
                     /old_locus_tag="pgaptmp_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGU4470387.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7577..8290
                     /gene="msyB"
                     /locus_tag="ACHOQQ_RS10675"
                     /old_locus_tag="pgaptmp_002135"
     CDS             7577..8290
                     /gene="msyB"
                     /locus_tag="ACHOQQ_RS10675"
                     /old_locus_tag="pgaptmp_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4470388.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8439..9005
                     /gene="satP"
                     /locus_tag="ACHOQQ_RS10680"
                     /old_locus_tag="pgaptmp_002136"
     CDS             8439..9005
                     /gene="satP"
                     /locus_tag="ACHOQQ_RS10680"
                     /old_locus_tag="pgaptmp_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGU4470389.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(9040..9627)
                     /gene="mog"
                     /locus_tag="ACHOQQ_RS10685"
                     /old_locus_tag="pgaptmp_002137"
     CDS             complement(9040..9627)
                     /gene="mog"
                     /locus_tag="ACHOQQ_RS10685"
                     /old_locus_tag="pgaptmp_002137"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGU4470390.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9742..10695)
                     /gene="tal"
                     /locus_tag="ACHOQQ_RS10690"
                     /old_locus_tag="pgaptmp_002138"
     CDS             complement(9742..10695)
                     /gene="tal"
                     /locus_tag="ACHOQQ_RS10690"
                     /old_locus_tag="pgaptmp_002138"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4470391.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10974..12404
                     /gene="yaaJ"
                     /locus_tag="ACHOQQ_RS10695"
                     /old_locus_tag="pgaptmp_002139"
     CDS             10974..12404
                     /gene="yaaJ"
                     /locus_tag="ACHOQQ_RS10695"
                     /old_locus_tag="pgaptmp_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGU4470392.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            12474..13250
                     /gene="yaaA"
                     /locus_tag="ACHOQQ_RS10700"
                     /old_locus_tag="pgaptmp_002140"
     CDS             12474..13250
                     /gene="yaaA"
                     /locus_tag="ACHOQQ_RS10700"
                     /old_locus_tag="pgaptmp_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGU4470393.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(13403..13699)
                     /gene="yaaX"
                     /locus_tag="ACHOQQ_RS10705"
                     /old_locus_tag="pgaptmp_002141"
     CDS             complement(13403..13699)
                     /gene="yaaX"
                     /locus_tag="ACHOQQ_RS10705"
                     /old_locus_tag="pgaptmp_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGU4470394.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(13913..15199)
                     /gene="thrC"
                     /locus_tag="ACHOQQ_RS10710"
                     /old_locus_tag="pgaptmp_002142"
     CDS             complement(13913..15199)
                     /gene="thrC"
                     /locus_tag="ACHOQQ_RS10710"
                     /old_locus_tag="pgaptmp_002142"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGU4470395.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(15200..16132)
                     /gene="thrB"
                     /locus_tag="ACHOQQ_RS10715"
                     /old_locus_tag="pgaptmp_002143"
     CDS             complement(15200..16132)
                     /gene="thrB"
                     /locus_tag="ACHOQQ_RS10715"
                     /old_locus_tag="pgaptmp_002143"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGU4470396.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVETAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDARLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(16134..18596)
                     /gene="thrA"
                     /locus_tag="ACHOQQ_RS10720"
                     /old_locus_tag="pgaptmp_002144"
     CDS             complement(16134..18596)
                     /gene="thrA"
                     /locus_tag="ACHOQQ_RS10720"
                     /old_locus_tag="pgaptmp_002144"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGU4470397.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18622..18741)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18677..18742)
                     /gene="thrL"
                     /locus_tag="ACHOQQ_RS10725"
                     /old_locus_tag="pgaptmp_002145"
     CDS             complement(18677..18742)
                     /gene="thrL"
                     /locus_tag="ACHOQQ_RS10725"
                     /old_locus_tag="pgaptmp_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGU4470398.1"
                     /translation="MKRISTTITTTITITTGNGAG"
BASE COUNT         4557 a         4957 c         4609 g         4755 t
ORIGIN      
        1 atgctatctg cgataaattt caccattcca tactctatcg catctgtatc agatagttcc
       61 aacaccgcag atatttttcc aggaactcct tttcctttgt tgttcgaaat tgaagtgagt
      121 tcatgtacag ggaataatac taattgtaat agcgtccgct ttaattttaa tttaaatact
      181 attttacagg taaatttaat gacctgtggt tttgaagatc aatccatcga cactggacga
      241 tttgtactgt ctcgtatcaa taatgaaaat tatcagtttc atcatataag atttgactgc
      301 atccagggcg agcaaggtga attagttttt gaacccagtg acgttgaata ttactttgaa
      361 ccagtgacga tacaggagtc aggaaccagc atattaaaaa atgagttaga aaacagtgag
      421 gacggtgcgg gtcaaatagg ctttcaactc tccaatgatg gtacgaatga aatccagtat
      481 ggtaaaagca attactactc ttttcagcat ccccatgaag gaagtaatca gattcctcta
      541 tttattcgcc cgcgtacata tggaaacaat gtctcaagcg ggcaaataat gagccgagtc
      601 aaaatcgtgg taatgtacaa ctaatctatc tcccccacct taactttggg ggagctgccg
      661 attaacgcac cgctggacta aaaagcgcag aataatccgt attgcagatt cgctgtaccg
      721 ccgggtgctg aatcatccgc tcagcaaaaa tagcatggta ctcttccatc acattctcga
      781 cgcgaccaat ttctacgaca gttttatcgg cataaaagtc atatgcataa agcgttgggg
      841 caacgaagat tgcattgtgc atcgcaccaa aagctttcat caaagcggca tcatcaaact
      901 cgccaaggat ttctacgttt aatccctggg agttaaacca gttaagtaat ttgcgcccta
      961 acattgaacg tcgcccagga atcaaaagcc gccgttcttc aagacaagct gggaacggtt
     1021 tttccggcgg tggatttgta caccagaaac tcacgccaca ttcgccaatt ctcacggaga
     1081 acaggccttc ctgctgcgta gaatctatcg gacagtcgga aatgatcata tccagtttat
     1141 gctggcttaa ttgctccagc agcatttcgt gggtggattc gaagcagcga agatgaatcg
     1201 gctcgccttc tactactgca gcattcagca cgctactgac cagacgtttg gaaagtgcat
     1261 cagccacgcc aacgtcaaac aataaattgg attctttgcg atagttcacg atatccagca
     1321 tttcctggct caaggtgaac attttatcgg catagcgata gaccagctcc cccagctcgc
     1381 tgggctcgag accacgtccc ttgcgcttaa ataatttgcc ttgcaggcgc tcttccagcg
     1441 cccgaatctg tccggtaatg gtttgtggtg ttaaataaag cgcctccgct gcgccaacca
     1501 cagaaccttc tttataaaca tgccagaagt aatacaagtg gttgtaattg atatgagaca
     1561 tgctcatttc tctccctgat aacaatgaaa agggagccgt ttatggctcc ccggtaaacc
     1621 gtcctgtcaa actgatggac gcaaacgaac gcgtaaccag ctgtatccaa ttaccgccga
     1681 agagatagaa ccgaccagga taccgagttt cgcccagtta atcagttctg gatctacgct
     1741 accaaaggcc aggctggcaa taaagataga catagtaaaa ccgataccgc acaggatccc
     1801 caccgccata atttgctgat aagtcgttcc ctcaggcaga tgcgccagtt tcaaacgcag
     1861 cgccaaccag cagaacagac taatccccag cggtttgcca atcagcaagc cagcgatgat
     1921 ccccaatggc agaatggagg tcaagccatc cagcgtgacg ccttgcagtg aaacgccagc
     1981 attagcaaat gcaaacagtg gtaaaatcag atacgccacc cacgggtgca acacatgctc
     2041 cagtcgcttc gctggagaac gcccatgctt ctctttcaaa ggaataaaga agccgacaat
     2101 tacccccgcc agggttgcgt gaacccccga tttcaacact gcagtccaca acactacgcc
     2161 aaccagaata tagacgcccg tgcggcgtac accacacaga ttcaataccg cgagtaccgc
     2221 aattgctaca gccgcgacgc caagagaggc catcgataag tcattagtgt agaacaatgc
     2281 gatgataatg atggccccaa gatcgtcgat aatagccaga gccatcaaaa agatcttcag
     2341 cgctaacgga acacgacttc ccaatagcgc cagcacacca agtgcaaagg caatgtcagt
     2401 agccgccggg atcgcccacc cttcgcgggt aatcggatcg gcatagttaa aggccagata
     2461 gagtaatgcc ggcacaatca tcccaccaat agcggcgata actggaaatg cggcctggcg
     2521 taagctggct agcgatcctt gcatcagttc acgtttaact tccagaccga ctaacaggaa
     2581 aaataccgcc atcagcgcgt catttatcca taacagcatg tttttgttga tttcgagtga
     2641 accaacccgg agctgaaccg gcgtctccag aaagtcgtga taccatccac tggttgcgcc
     2701 gctgttggcc atcatcatcg ccaggatagc ggcaatgata agaataatgc ctcccgaggc
     2761 atcactgcta aagaatcgat gcagatgttt cactttttat ttctctttca ggtgaataga
     2821 tcgtttttac tattttaccc cgcacgaatc atcgttaaaa tcagattata gtgtaggaat
     2881 aattcgtttt ttacgatcta tagaagaatt tgcgccatca gcggtgagat gcgtttttga
     2941 cggctgtaag cgctgacggg tgaccctaag ccctgataat catagcttaa ggtgaggatt
     3001 tctgagggat taattaggaa aagtaataat taatttgttg tcagtcggcg atgtttgtta
     3061 ggccaatatt tcaaaaataa tccagcttgt cgtcaataat tgcgatgaag agataaatta
     3121 aagttaaata attaaatcat taaaaaatta cgccataaaa atgcgaggcg tcttccggat
     3181 tagaatcgat aaaacagaat gggttaaaga gagcgatata atagcggcgg gtgcttgagg
     3241 ctgtctgtct caggcattag ctgaacggca gatagagaaa agccccgagt gatattttac
     3301 catcaacccg aggcccccta tatgctgaac acatgtagag tgcctcttac tgaccgtaag
     3361 gtcaaggaga agagagcaat gaagcagcat aaggcgatga ttgtcgccct gatcgtcatc
     3421 tgtatcaccg ccgtagtggc ggcgctggta acgagaaaag acctctgtga ggttcacatc
     3481 cgaactggcc agacggaggt tgctgttttc acggcttacg aatccgagta agagcaacgg
     3541 cggggagtga tccccgccac tttacaggtg ctcgcatatc ttcaacgcac cctattttta
     3601 cccaggcctg cccacgggca ggcttttggg gaggttagcg ggtcaggtcg tcaaaaaact
     3661 tcttcacacc atcaaagaag ctctttgagc gcgggctgtt gtgctcgccg gttgggccac
     3721 cgaagctttc ttgcagctct tgcagcagct gtttctgctt ctcgttcagg cctacaggtg
     3781 tttcgacgac aacgcggcac agcaaatcac cctgtgcgcc accgcggaca gacttgacgc
     3841 ctttaccgcg catacggaat agcttaccgg tctgggtttc gccaggcact ttcagtttga
     3901 cgcgaccatc aagggtcggt acttcgattt cgccacccag cgccgccata gcgaagttga
     3961 tcgggacttc gcaatacagg ttgttgcctt cacgctcgaa aatcgggtgc tgtttaacct
     4021 gaacctgaac gtacagatcg cctgccggtg cgccatgctc gcccgcttca ccttcgcccg
     4081 caagacggat gcggtctcca gtgtccaccc ctgccgggat tttaacggac agcgttttgc
     4141 tgcgctcaac acgaccatga ccatgacatt tgttgcacgg atctttgatc agcgtaccgc
     4201 ggccctgaca gtgtggacag gtctgctgca cggcaaagaa accctggcgc atctgcacct
     4261 gaccagaacc atgacaggtc ggacaggtct gcggctgtgt acctggtttt gcaccgctac
     4321 cgtggcaaac gtcacactct tccagagtcg gaatgcggat ctctttggtc acgccacgta
     4381 cagcttcttc gagggtgagc tccatgttat agcgtaaatc agcaccgcgc gccgcacgtt
     4441 gacgaccacg tccgccgcca aaaatatcgc cgaaaacgtc accaaaaata tcgctgaagt
     4501 ctgcgccgcc gccaaaaccg ccgccgccca tgccaccttg ctcaaacgca gcatgaccat
     4561 actgatcgta tgccgcacgt ttttgcgagt cggtcagaac ttcataagct tccttgatct
     4621 ctttaaattt cgcctcggcc tctttgtcac cctggttacg gtccgggtgg tatttcatgg
     4681 ccaggcgttt gtaggccttt ttgatttcac gctcttccgc tgttttggaa acgcctaaaa
     4741 tctcgtaata atcttgctta gccatctttt ttaaattgcc cctagatgaa tgcacgggcg
     4801 gagaggaaat tccccttcgc ccgtgtcagt ataattaccc gtttacaggg cgattatttt
     4861 ttgtctttga cttcttcaaa ctcagcgtcg acaacatcgt catctttcgc gttgtttgca
     4921 gaagcatcag caccggcagt ctgctgctgg gcatgttgct gctgggcgat ttccatcagt
     4981 ttctgggaaa cctgtgccag ctcctgcatt ttcgcttcga tagcggcttt gtcttcacct
     5041 ttcagagcag tttccagtgc agtcagcgca gactcgatag cagttttgtc gtcagccggc
     5101 agtttgtcgc ctgcttcttc aacctgctta cgggtgctgt gcagcagatg gtcgccctgg
     5161 ttgcgagtct gtaccagctc ttcaaactta cggtcagctt cggcgttagc ttctgcgtcg
     5221 cgtaccattt tctggatttc atcttcgttc agaccagaag aagccttgat ggtgatcttc
     5281 tgctctttac cgctgttttt atctttcgcg gaaacgtgca ggataccgtc agcatcgata
     5341 tcgaaggtaa cttcgatctg cggcataccg cgcggtgccg ggttgatacc atccaggttg
     5401 aactgaccca gagatttgtt atcagccgca cgtttacgtt caccctgcag cacgtggatg
     5461 gttaccgcag actggttgtc ttcagcggta gagaacacct ggctgtgctt ggtcgggata
     5521 gtggtgtttt tcgcgatcag cgtcgtcatc acaccgccca tggtttcgat acccagagac
     5581 agcggggtaa cgtccagcag cagtacgtct tttacgtcac cagtcagaac accaccctga
     5641 acagcagcac cgattgctac agcttcgtcc gggttaacgt ctttacgcgg ctctttacca
     5701 aagaactcag caactttctt ctgaaccatt ggcatacgag tctgaccacc aacgaggata
     5761 acgtcgtcga tatcagatac ggacaggcca gcgtcctgca gtgcaacttt cagcggctca
     5821 atggaacggt ttaccagatc ttcaaccagg ctttccagtt tcgcacgagt cactttgatg
     5881 ttcatgtgtt tcggaccggt cgcgtctgca gtgatgtacg gcaggttaac gtcggtctgc
     5941 tgagcggaag acagttcgat tttcgctttt tctgccgctt ctttcaggcg ctgcattgcc
     6001 agcggatcgt tgcgcaggtc aatgccctga tctttcttga attcttcaac cagatagttg
     6061 atcagacggc tgtcgaagtc ttcaccaccc aggtgggtat caccgttggt tgccagaact
     6121 tcgaaggttt tttcgccgtc aacttcgtcg atttcaataa tagaaatatc gaaagtacca
     6181 ccacccaggt cataaaccgc gatagtacgg ttgccagtac ctttgtccag accgtaagcc
     6241 agcgcagctg cagtcggttc gttgatgata cgttttactt cgagaccagc gatacggcct
     6301 gcgtctttgg ttgcctgacg ctgagcatcg ttaaagtatg ccggtacggt gataacagct
     6361 tcagttaccg gttcacccag gtaatcttca gcggttttct tcattttttt cagcacttca
     6421 gcagaaatct gcggcggtgc cattttctgg cctttaactt cgacccatgc gtcgccgtta
     6481 tcagcagcaa taattttgaa cggcatgatg gaaacatcac gctgtacttc ttcgtcctgg
     6541 aagcggcggc caatcaggcg tttaatcgca aacagggtgt tttgcgggtt cgtcactgcc
     6601 tgacgtttag ccggctgacc aaccagagtt tcaccatcct gggtataggc aatgatagaa
     6661 ggcgtggtgc gatcgccttc ggcgttctcc agcacacgag gagtggtgcc atccataatc
     6721 gctacacaag agttggtagt acccaggtcg ataccaatta ttttacccat ctaaacgtct
     6781 ccactatata ttcggtcatc atgtggttgt gagtctgtaa taggggcgaa acgtctggtt
     6841 tcaactgccc aatttcattt ttttgcagac tcactcactg cggttgacta ctaaatgggg
     6901 tcgtaaccca cgtcatcaag ggggagatgc aaaaattttt tgtgcttttg cgcagaaatt
     6961 tcgcgatatg gcaggacaag acgtaaaatt gacagcaatt ttattcatat aaagaatgaa
     7021 tcgcagtcaa ggtattcatc aaaaaattgt aataaaaaga aaagattacg tgcctgaatc
     7081 ttctctttat cagcagtaaa ctagtgggta ttcatccccc tacctcttcc cactaagaga
     7141 atccttatga aatccgtttt tacgatttcc gccagcctgg cgattagcct gatgctgtgc
     7201 tgcacggcgc aggcaaacga ccataaactc ctcggcgtca ttgcaatgcc gcgtaacgaa
     7261 accaacgatc tggcgctgaa acttcctgtt tgtcgcattg tgaaacgcat acaactctct
     7321 gccgaccatg gcgatttaca gttaagcggt gcatcggttt atttcaaagc cgcccgtagc
     7381 gccagtcaga gcctgaatat tccttcagaa ataaaagaag ggcaaaccac tgactggatc
     7441 aacattaaca gcgataacga caataaacgc tgcgtctcaa aaatcacctt ttccggtcat
     7501 acggtgaact catcggatat ggccacgctg aaaattatcg gcgacgacta acgccagaat
     7561 atagaagcca caaaaaatga atgttaatta cctgaatgat tcagatctgg attttctcca
     7621 gcattgtagt gaggaacagt tagcaaattt cgcccggctg ctcacccata atgaaaaagg
     7681 caaaactcgc ctctccagcg tactgatgcg caacgaactg tttaaatcga tggaaggaca
     7741 tcccgagcaa catcgccgca actggcagct gattgccgga gaattacagc attttggtgg
     7801 cgatagtatc gccaacaaac tgcgcggaca cggtaaattg tatcgggcca ttttgctcga
     7861 tgtttcaaag cgattgaagc tgaaagccga caaagagatg tctacgtttg aaattgagca
     7921 acagttactg gaacaatttc tgcgtaatac ctggaagaaa atggacgagg aacataagca
     7981 ggagtttctg cacgcggtcg atgccagggt gaatgagctg gaagagctgc tgccgctgct
     8041 gatgaaagac aaattattgg caaaaggtgt gtcgcatttg ctttccagcc aattgacccg
     8101 cattttacgc acccacgcag caatgagcgt gcttgggcat ggtttgctgc gcggcgcggg
     8161 tctgggaggc cctgtgggcg cagcattaaa tggggttaaa gcggtcagcg gcagcgccta
     8221 tcgcgtgacg attccagccg tactgcaaat cgcctgcctg cgccggatgg ttagcgccac
     8281 tcaggtctga taccggaaac cggggaaaat tttcattttc ccctcaaaag atcgtagaca
     8341 ctgccccact ggctgattat tatgccgcgc cctgaaaaca ctacagttat tcagggaaat
     8401 tatttcacca ttcattcgat gatgattttt gaggaattat gggcaacact aagttggcta
     8461 atccggcacc gctgggcctg atgggcttcg gcatgaccac cattctgctt aacctgcaca
     8521 acgtgggtta tttcgctctg gacggtatta ttcttgccat gggcattttc tacggcggca
     8581 tcgcgcaaat ttttgccggt ctgctggagt acaaaaaagg caacactttc ggtttaaccg
     8641 cattcacctc ttacggttct ttctggctga cgctggttgc gattctgctg atgccgaaac
     8701 tgggtctgac cgatgcgcca aatgcacagt tccttggtgt ctacctgggt ctgtggggcg
     8761 tatttacgct gtttatgttc ttcggcacgc tgaaaggcgc acgcgtcctg caattcgttt
     8821 tctttagcct gaccgtgctg tttgccctgc tggcgatcgg taacattgcc ggtaatgccg
     8881 cgatcatcca ctttgccggc tggattggtc tgatctgcgg tgccagcgca atctatctgg
     8941 cgatgggtga agtgctgaac gagcagtttg gtcgcaccgt tctgccgatt ggtgaatccc
     9001 actaattgtt ttgagatccc cccgctcggg gggatttttt tattcgctaa cgtcgcgtct
     9061 tgcactcttc ggtctgaatg cagcaaccac ttctggtgcc gtttcaacgt atggcccttc
     9121 cagcaactga atgcagtacg gtacgctggc aaaaataccg tgtaccacaa cgttaccctc
     9181 agcgtccttc acaccttcca gcgtctcttt aatagacttc ggctgaccgg gtaagttaag
     9241 gatcagcgcc tgtttgcgaa tcacacccac ctgacgcgaa aggatcgcag ttggtacaaa
     9301 atgcaggctg atctggcgca tctgttcacc aaagccaggc atctcgcggt ccgctactgc
     9361 cagcgtcgca tcgggcgtta cgtcacgacg cgccgggcca gttccgcccg tggtgagcac
     9421 cagatggcaa ctcatttcat ccaccagctc acacaacgtt tgctcgatga tcgcctgctc
     9481 atcggggatt aagcgggttt ccagttcaaa cggcgtggtt agcgccgatg tcagccattc
     9541 ttccagcgca gggatgcctt tatcctgata aacgccactg gatgcgcgat cagagatgga
     9601 aactaagcca atacgtaaag tattcatcaa gatttttccg tttaaacagt ctcgttaaac
     9661 agaatgatac actgcgaagg gagtgacaga caggcttcag aagaggtagc gtgaccgact
     9721 tcccggtcac gctaagaatg attacagcag atcgccgatc attttttcca gtttttcctg
     9781 gtcaatagca aacttacgga taccttccgc cagtttatct actgccattg gatcctggtt
     9841 gtgctgccac aggaactcgg actcagtgat acgcgccgga cgcgctttca cttcgccggt
     9901 gtaagacagt ttacgttcga tagccccttc gctctcagcc agctctttca gcagtgccgg
     9961 tgcgatggtc agacggtcgc agcctgccag ttccagaatt tcgccgatgt tacggaagct
    10021 tgcgcccata accacggttt cataaccgtg ctctttgtag tactggtaga tttcagatac
    10081 agaaaccacg cccggatctt ctgccggagc gtactctttc ttatcggtat tcgctttgta
    10141 ccagtcaaga atacggccaa caaatggaga gatcagaaac acgcccgctt ccgcacaagc
    10201 acgagcctga gcgaaggaga acagcagggt caggttacag ttgatacctt ctttttccag
    10261 ctgttccgca gcacggatac cctgccaggt agaagccagt ttgatcagaa tacgatcgtt
    10321 gctaatacct gcatcgttgt agagtttgat caggcgtttt gctttcgcaa ttgacgcttc
    10381 ggtgtcatag gaaagacgcg catcaacttc agttgagata cggcccggaa ccagtttcag
    10441 gatttccaga ccaatattta ctgccagttt gtcagtcgcg tccacgatct gctgcgcgcg
    10501 atcgttgctc tgctgtttcg cccaggcgac agcatcatca atcaacttac gatattccgg
    10561 aatctgcgct gcgttaagaa tgagagaagg gttggttgtg gcatcctgcg gttgatacag
    10621 cttcattgcc gcgatgtccc cagtgtcggc cactacggtg gtgtactgac gaagggaggt
    10681 caatttgtcc gtcatgatag tatttctctt taaacagctt gttaggggga tgtaaccggt
    10741 ctgccctgat gatatcacga cgcaacgtca gcgcaaccgc cgacaagacg gttatcgcca
    10801 ggcgagactg tttcggattt ctgacactgc gttgatatcg cccgccattt ttaaacaaaa
    10861 cctcatgtat gctacgcaga agttatcaag tacctcgtag cgtatatact tcttaaacaa
    10921 ttggtaacgt ttacacagga aagtcatcgc gaccggcaat aagagggata tgcatgccag
    10981 attttttctc ctttattaac agcgtccttt ggggatcggt aatgatttac ctgctcttcg
    11041 gcgcaggttg ttggttcact tttcgcaccg gatttgtgca gtttcgctac atccgccagt
    11101 ttggcaaaag tcttaaaaat agcattcatc cacagccagg cggtttaacc tcatttcagt
    11161 cattgtgtac cagtcttgcg gcgcgcgtgg gtagcggcaa cctggccggc gttgcgctgg
    11221 ctattaccgc cggtggacct ggagccgtct tttggatgtg ggttgccgcg tttatcggca
    11281 tggcgacctc gtttgccgaa tgttccctgg cacaacttta taaagaacgt gacgtcaatg
    11341 ggcagtttcg tggcggaccg gcatggtata tggcgcgcgg gctggggatg cgctggatgg
    11401 gcgttctgtt cgccgtcttt ttgctcatcg cctacggcat aattttcagc ggagttcagg
    11461 caaacgctgt tgcccgagcc ctgagttttt cttttgattt tcccccgctg gtgacaggca
    11521 ttattctcgc tgtctttgct ctgctggcaa tcactcgcgg tcttcatggc gtcgcccggc
    11581 tcatgcaggg gtttgtcccg ttgatggcga taatctgggt actgaccagc ctggtgattt
    11641 gcgtaataaa tatcgggcaa cttccccacg tcatttggtc tatttttgag agtgcttttg
    11701 gctggcagga agcggcaggc ggcgcggcgg gatatacctt aagccaggcg attactaacg
    11761 gttttcagcg cagtatgttt tccaatgagg cgggaatggg ttcgacgcca aacgcggcag
    11821 cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg aattgtccag atgattggca
    11881 tttttatcga caccctggtc atctgtacgg caagcgccat gctgatatta ctggcgggta
    11941 acggcacaac ctacatgccg ctggaaggta ttcagcttat ccagaaggcg atgcgggtgc
    12001 taatgggttc ttggggtgct gagtttgtta ccctcgtggt tattctgttt gccttcagct
    12061 ccatcgttgc caactacatt tatgccgaaa acaatctctt ctttttacgc ctgaacaacc
    12121 ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc ggcaccttgc
    12181 taagtcttcc gctgatgtgg caactggcag atatcataat ggcctgcatg gctattacca
    12241 atttgaccgc cattttactg ctctcgcctg tggttcatac cattgccagt gattatctac
    12301 gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg gatatcggtc
    12361 gccagctttc tcgggacgcg tgggatgatg tttcgcagga gtaatcacaa ctatcgatca
    12421 actcattctc gttttttgct aaagtcggca taaatttcct gcaaggactg gatatgctga
    12481 ttcttatttc acctgcgaaa acgcttgatt accaaagccc gttgaccacc acgcgatata
    12541 cactgccgga gctgttagac aattcccagc agttgatcca tgaagcgcgg aaactgacgc
    12601 ctccgcagat tagcacgctg atgcgtatca gcgacaaact ggcaggtatc aacgccgctc
    12661 gctttcatga ctggcagcca gatttcacgc cggagaatgc ccgccaggcg attctggcgt
    12721 ttaaaggtga tgtctacacc ggcttgcagg ccgaaacctt cagcgaagac gatttcgatt
    12781 ttgcccaaca gcatttgcga atgctttccg gcttgtatgg cgtactgcgc ccgctcgatt
    12841 taatgcagcc ttatcgtctg gaaatgggga tccgtcttga gaatgcccga gggaaagatc
    12901 tgtatcaatt ctggggagat atcatcacca acaagctgaa cgaggcgctc gcagcacaag
    12961 gcgataatgt ggtgattaat ctggcgtcag atgagtattt taaatcggtg aagccgaaga
    13021 aattgaatgc cgagattatc aagccggtgt tcctcgatga gaagaacggc aaatttaaga
    13081 taatcagctt ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt attgaaaatc
    13141 ggctgaccaa accagagcaa ctgactggtt ttaatagcga aggttacttc tttgatgaag
    13201 cttcctccag caatggcgaa ctggtgttta aacgctacga gcagcgttaa gtttgtagtc
    13261 aatgccggat gcggcgtgga cgccttatcc ggcctacaaa agcatgcaga ttcaatatat
    13321 tgcagaattc acgtaggcct gataagacgc gccagcgtcg catcaggcgc taaatgcagg
    13381 attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga ccgccgtgat
    13441 gatcatgatg agctttctta tggtgacgcg gcggtggcgg cggtccgtgt gggtgccagc
    13501 gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag tgaccgccat
    13561 cccagtaata accgcgatta tcacgatcgc ctatctgtaa ttttactgac gggactaacg
    13621 taatttccgc agcctgcgct gccatgggag cgaccagaac cagggaaagt gcgagtacaa
    13681 tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg gtcgatatcc
    13741 ggagattact cgatgagaac ggcggttgtt attctccgca atcctaatct ctaagttacc
    13801 gcatttattg agaatttctc ctttttccct gtcgtttttc tccataattt cttcataaaa
    13861 aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga tattactgat
    13921 gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgc gaaagcaagg
    13981 gtaaatcggc acgttctgcc agctcttttg gcagatccaa cgtttcaccg agaatcgctt
    14041 ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca tattcgcctg
    14101 gattcaactg gtcacgcagc gcacgatacg ctacggcagc gtgcggctcc gaagtgtagc
    14161 ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg gctgcataac
    14221 ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc ggccagttgt
    14281 tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg ggtgaccact
    14341 gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca ataaaacgtt
    14401 tcaccggcag accgagtgac ttcgccagca gacccgccgt caaatcgccg aagtttccgc
    14461 ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg cagctgcgca actgcttcaa
    14521 agtagtagca aatctgcgcc agcaaccggc tgatgttaat cgagttagcc gagtttaacc
    14581 ccagcgccac tttcagttct tcatcatcaa acgcctgctt caccagcgcc tgacaggcat
    14641 cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caatgtacag aacagttttt
    14701 cttgcagtgg actgattttg cctcgtggat agaggataac cactttcaca ttcggtaaac
    14761 cgtagaaagc atgagccact gccgctccgg tatcaccgga ggtcgcggtc agaatggtca
    14821 ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg ccgaaatctt
    14881 taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt tcaacattgg
    14941 cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt tcctgcggga
    15001 tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa tccagcttca
    15061 gcatctcatc aatttcagtc aggctgaatt ccggcaggtc gtgcggaaaa aacagcccct
    15121 gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctca ttgtgatctt
    15181 tcagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta tccagccggc
    15241 aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag tcggcaacgc
    15301 gctgggcggt atccggcttg tcacacagag cgaacaaggt cgggccggag ccggagatac
    15361 cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag ccaggcagta
    15421 accgttcacg gtagggttct gcgataacat ctttcatcag cttcgcggca agctcaggct
    15481 gacgggaata gcaagcgtga atgaagcctg ccagatgtcg cccgtgcgca atgcaatcct
    15541 ggcggcgata ctgcgccggt aaaatagccc gggcttctgc cgtcgagact ttaatccccg
    15601 gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg atgatgtcgt
    15661 tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg tcgtaatgaa
    15721 tgctgccgga gatacgccct tccagctcgc ccatcaaagc cagcaaacga gcgtcattaa
    15781 gcggcttgcc gcagtgttca ttcatcgcca tcagcgccgc gaccaccgaa caggcgctgg
    15841 agcctaagcc cgaaccgatc ggcatattct tttccagagt catcgccact ggaatttgct
    15901 tgcccagctc ctggcaaaaa cgctcccagc actgataaac gatattttcc cgtggttctg
    15961 acggcagctt atcggcaaaa cgtccgaggt tgttgaggct gaatgtctct gccgtctcaa
    16021 ccgtgactac atctccgagc aatgcaccat caacgggtgt caccgccgcc ccgagcacat
    16081 caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc atgtcagact
    16141 cctagcttcc atgagagggt acgtagcaga tcggcaaaga caccggcagc ggtaacgtca
    16201 ttgcccgcac catatccgcg cagcaccaat ggcagcggct gataatagtg gctatagaag
    16261 gccagggcgt tttcgccatt tttcactttg aacagcggat cattaccatc cacttcggca
    16321 atcttcacgc ggcagacgcc atcttcatca atattgccaa catagcgcaa aacttttcct
    16381 tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga cagattcgcc
    16441 ataaaagcgg caacatcacc ctcggcgtta aactctgcgg gcagcactgg ttcaatttca
    16501 atatccgcca actccagttc acgtcccgtt tcacgagcga gaattaatag tttacgcgct
    16561 acatccatac cagaaagatc atctcgcgga tccggttcgg tataacccat ttcccgcgcc
    16621 agagtagtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat ataagaaagc
    16681 gaacctgaaa gaatgccgga gaacttcatc aattcatcac cagcattgag cagattttgc
    16741 aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa tttacgtcgc
    16801 gatttttccg ccgcatgacg caacagatgg tagtaatcca tcgacgaggt gttggccttt
    16861 ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg atccgccact
    16921 gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc tttcacgagg
    16981 cgaattaagc gcccgagatt aaacggctct ttggcttgcg ccagttcttc ctgccagttt
    17041 tccaggttta ggccatgcac attggtaagc agagccttcg agttagcaac accgcagaca
    17101 cgtaagtcga tatgtttatt cttcaaccag ctttgctgac gcttcagttg ctccagcagc
    17161 gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc ggtattgaac
    17221 agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac cacgacagag
    17281 attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt tgatattggc gcgggccagc
    17341 gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc taccaccgag
    17401 ataatggcca gccgttccgt caccgccagc ggctccagta agccttcttt cagttccagg
    17461 tagaactctt cctgcattgc ccgttcagct cgcacacagt cgctttgcgg aacgcagaaa
    17521 ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg ggcgcgtgac
    17581 attgcagcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg gccggaaacg
    17641 ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc gtcttcatca
    17701 cgactggcac caatgagcgt acctggtgct tgaggatttc cggtattttt aatcaggcac
    17761 gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt agcgccgaag
    17821 taggaaagct ccatcgcttc ctggtaggac atcgatttca acaacctcgc atcgggcacc
    17881 tgacgcgggt cgcaggtata aaccccgtca acgtccgtcc aaatctcgca acaatcggcg
    17941 cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc aagtaccacc
    18001 agttcgcctt tttcattacc ggcggtgaaa cctgccatca gcaccatgtg atcagccgga
    18061 atacgacttg ccgcaatacg gcgggtggac tcagcaatat cgacggtaga ttcgaggtaa
    18121 tgccccactg ccagcagttt ttcgaccgga tcgataacgg taacgttgtg accacgcgct
    18181 tctaacacgc cggccataat ggcgattgac attttctcgc cacggcaaat cagcgcagcg
    18241 ttaatgctat ccgggcactg ccctaacaaa ctaatgccat gcaggacatg ttttatttgg
    18301 gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg ggaatcccgg ctgggcggcg
    18361 gcgagtcccg tcagaagttc ggcaaaaata cgttcggcat cgctgatatt gggtaaagca
    18421 tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggttat tttggcgggg
    18481 gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat atcggcaacc
    18541 cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac tcgcatggtt
    18601 gttacctcgt tacctttggt cgaaaaaaaa gcccgcactg tcaggtgcgg gcttttttct
    18661 gtgtttcctg tacgcgtcag cccgcaccgt tacctgtggt aatggtgatg gtggtggtaa
    18721 tggtggtgct aatgcgtttc atggatgttg tgtactctgt aatttttatc tgtctgtgcg
    18781 ctatgcctat attggttaaa gtatttagtg acctaagtca ataaaatttt aatttactca
    18841 cggcaggtaa ccagttcaga agctgctgtc agagactc
//
LOCUS       DAZKFU020000078        18806 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZKFU020000078 DAZKFU020000000
VERSION     DAZKFU020000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.078"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..752
                     /gene="yqfA"
                     /locus_tag="ACHOQQ_RS10730"
                     /old_locus_tag="pgaptmp_002146"
     CDS             93..752
                     /gene="yqfA"
                     /locus_tag="ACHOQQ_RS10730"
                     /old_locus_tag="pgaptmp_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGU4470399.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(829..1809)
                     /gene="ygfZ"
                     /locus_tag="ACHOQQ_RS10735"
                     /old_locus_tag="pgaptmp_002147"
     CDS             complement(829..1809)
                     /gene="ygfZ"
                     /locus_tag="ACHOQQ_RS10735"
                     /old_locus_tag="pgaptmp_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGU4470400.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            2052..2318
                     /gene="sdhE"
                     /locus_tag="ACHOQQ_RS10740"
                     /old_locus_tag="pgaptmp_002148"
     CDS             2052..2318
                     /gene="sdhE"
                     /locus_tag="ACHOQQ_RS10740"
                     /old_locus_tag="pgaptmp_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGU4470401.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            2299..2706
                     /gene="ygfX"
                     /locus_tag="ACHOQQ_RS10745"
                     /old_locus_tag="pgaptmp_002149"
     CDS             2299..2706
                     /gene="ygfX"
                     /locus_tag="ACHOQQ_RS10745"
                     /old_locus_tag="pgaptmp_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGU4470402.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(2746..3267)
                     /gene="fldB"
                     /locus_tag="ACHOQQ_RS10750"
                     /old_locus_tag="pgaptmp_002150"
     CDS             complement(2746..3267)
                     /gene="fldB"
                     /locus_tag="ACHOQQ_RS10750"
                     /old_locus_tag="pgaptmp_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGU4470403.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            3379..4275
                     /gene="xerD"
                     /locus_tag="ACHOQQ_RS10755"
                     /old_locus_tag="pgaptmp_002151"
     CDS             3379..4275
                     /gene="xerD"
                     /locus_tag="ACHOQQ_RS10755"
                     /old_locus_tag="pgaptmp_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGU4470404.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            4300..5010
                     /gene="dsbC"
                     /locus_tag="ACHOQQ_RS10760"
                     /old_locus_tag="pgaptmp_002152"
     CDS             4300..5010
                     /gene="dsbC"
                     /locus_tag="ACHOQQ_RS10760"
                     /old_locus_tag="pgaptmp_002152"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGU4470405.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            5016..6749
                     /gene="recJ"
                     /locus_tag="ACHOQQ_RS10765"
                     /old_locus_tag="pgaptmp_002153"
     CDS             5016..6749
                     /gene="recJ"
                     /locus_tag="ACHOQQ_RS10765"
                     /old_locus_tag="pgaptmp_002153"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGU4470406.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6840..7938
                     /gene="prfB"
                     /locus_tag="ACHOQQ_RS10770"
                     /old_locus_tag="pgaptmp_002154"
     CDS             join(6840..6914,6916..7938)
                     /gene="prfB"
                     /locus_tag="ACHOQQ_RS10770"
                     /old_locus_tag="pgaptmp_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGU4470407.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7948..9465
                     /gene="lysS"
                     /locus_tag="ACHOQQ_RS10775"
                     /old_locus_tag="pgaptmp_002155"
     CDS             7948..9465
                     /gene="lysS"
                     /locus_tag="ACHOQQ_RS10775"
                     /old_locus_tag="pgaptmp_002155"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4470408.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFGGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(9508..10056)
                     /gene="idi"
                     /locus_tag="ACHOQQ_RS10780"
                     /old_locus_tag="pgaptmp_002156"
     CDS             complement(9508..10056)
                     /gene="idi"
                     /locus_tag="ACHOQQ_RS10780"
                     /old_locus_tag="pgaptmp_002156"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGU4470409.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNPVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            10111..10182
                     /locus_tag="ACHOQQ_RS10785"
                     /old_locus_tag="pgaptmp_002157"
     CDS             10111..10182
                     /locus_tag="ACHOQQ_RS10785"
                     /old_locus_tag="pgaptmp_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470410.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(10179..10304)
                     /gene="yqfG"
                     /locus_tag="ACHOQQ_RS10790"
                     /old_locus_tag="pgaptmp_002158"
     CDS             complement(10179..10304)
                     /gene="yqfG"
                     /locus_tag="ACHOQQ_RS10790"
                     /old_locus_tag="pgaptmp_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGU4470411.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(10306..11754)
                     /gene="uacT"
                     /locus_tag="ACHOQQ_RS10795"
                     /old_locus_tag="pgaptmp_002159"
     CDS             complement(10306..11754)
                     /gene="uacT"
                     /locus_tag="ACHOQQ_RS10795"
                     /old_locus_tag="pgaptmp_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGU4470412.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            12190..14109
                     /gene="ygfT"
                     /locus_tag="ACHOQQ_RS10800"
                     /old_locus_tag="pgaptmp_002160"
     CDS             12190..14109
                     /gene="ygfT"
                     /locus_tag="ACHOQQ_RS10800"
                     /old_locus_tag="pgaptmp_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGU4470413.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHI
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGCDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            14109..14597
                     /gene="ygfS"
                     /locus_tag="ACHOQQ_RS10805"
                     /old_locus_tag="pgaptmp_002161"
     CDS             14109..14597
                     /gene="ygfS"
                     /locus_tag="ACHOQQ_RS10805"
                     /old_locus_tag="pgaptmp_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4470414.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDCISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLLGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     LL"
     gene            complement(14633..16000)
                     /gene="ghxQ"
                     /locus_tag="ACHOQQ_RS10810"
                     /old_locus_tag="pgaptmp_002162"
                     /gene_synonym="ygfQ"
     CDS             complement(14633..16000)
                     /gene="ghxQ"
                     /locus_tag="ACHOQQ_RS10810"
                     /old_locus_tag="pgaptmp_002162"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGU4470415.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(16036..17352)
                     /gene="guaD"
                     /locus_tag="ACHOQQ_RS10815"
                     /old_locus_tag="pgaptmp_002163"
     CDS             complement(16036..17352)
                     /gene="guaD"
                     /locus_tag="ACHOQQ_RS10815"
                     /old_locus_tag="pgaptmp_002163"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGU4470416.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(17370..18770)
                     /gene="xanQ"
                     /locus_tag="ACHOQQ_RS10820"
                     /old_locus_tag="pgaptmp_002164"
     CDS             complement(17370..18770)
                     /gene="xanQ"
                     /locus_tag="ACHOQQ_RS10820"
                     /old_locus_tag="pgaptmp_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGU4470417.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
BASE COUNT         4782 a         4687 c         4938 g         4399 t
ORIGIN      
        1 gaatttattt tagctaacag gtgttcactg gaactattct cagttacgct ggagaggtat
       61 acatgtgcgt gtacgtttct ccggagtaag ttatggttca gaagcccctc attaagcagg
      121 gatattcgct ggcagaggaa attgccaaca gcgtcagtca cggcattggg ttggtgtttg
      181 gtatcgttgg gctggtgttg ctactggttc aggcggtgga tctcaatgcc agcgccacag
      241 cgataaccag ctacagcctc tatggcggca gtatgatcct gctgttcctc gcttcgacgc
      301 tctatcacgc cattcctcat caacgggcaa aaatgtggct gaagaaattt gaccattgcg
      361 ctatttacct gttgattgcc ggaacctaca cgccgttttt gctggtgggg ctggattctc
      421 cgttagcgcg cgggttgatg attgttatct ggagcctggc attgctgggg attctgttca
      481 aactgaccat cgcgcaccga ttcaaaattt tatctctggt gacttatctg gcgatgggct
      541 ggctgtcgct ggtggtaatt tatgaaatgg cagttaagct cgcggcgggc agcgttacct
      601 tactggcggt aggcggcgtg gtttattcgc tcggggtgat tttctacgtc tgcaaacgca
      661 ttccatacaa ccatgccatc tggcacggct tcgtgctcgg cggtagtgtg tgccactttc
      721 tggcgatcta tttgtatatt gggcaggcgt aatgggtatg cctttgtagg cctgataaga
      781 cgcgccagcg tcgcatcagg cgataaatgc ctgatgcgac acagagattt actcttcgag
      841 cgaatacggc agcggctcga tatgcaatgt attcgcatcg tcacgtacgc ggaagatgct
      901 gtccggttcc atatcgttat tcatcactac ctgtaccacg acctgaccat cttccagttt
      961 taccgcagcc agcactgtac cggtacgacg ccagttctcg cccattttca gctctaagtc
     1021 ttcaccagct tccggcagtc ggctggcact acctgtcagc aaccagagcg cacgtttatt
     1081 ggcaccacgg aattttgctc gcgccaccat ctcttgtccg gtataacagc ctttcttaaa
     1141 gctgataccg cccagcgcct ggagattggt cgcctgtggg ataaactgcc cgctgttcgc
     1201 ggcatcaatc accgggaaac ccgcttcaat gtttaatgcc agccactgtt ggctattgtt
     1261 cagttccgct tcaccgcgca gtttatcggt cagcatattg gcagtagctt catcggttac
     1321 gatcaggaaa cgttctgccg ggtgttcaaa ccatagcaaa gtggtcgcgc cttctttaac
     1381 tacctgtttt tctctcgaag gtagttcact aaagagattg gccagcgcgg cgcgcgcctg
     1441 aaaaccggca acaccaagca gcacacgctc gtcgtctggc gcgatggtca ctttagagaa
     1501 caccgcatat tttttcagtt cagtcagctg cggttcacgc acgctgcgcc gttcaatcca
     1561 cgcaaagcca tcgccgtcgc ggaacagacg taaattgctc cacattttac ctttggcgtc
     1621 gcaatgggcg gcgagcaggt gctgattttc tgtcatctgg ctgacatctg ctgtcacctg
     1681 gccctgcata tatttttcgc tgtccgcgcc agtaatggtg gcaagcgccc agtcatcaag
     1741 cgtcatcagc gtcagcggta aacgggcaga agccgtaggc tgacgtggag gaaaaggtgt
     1801 aaaagccata atagagtcct gagttgctta acggcgtatt aataactaat ggtaaaagag
     1861 ctatgaggca atgcaagtgc tttcactacg cgatctgtgc cttctgcgag gcgacttcca
     1921 aaaaaatgaa gcccttgaat tgttgtgaat taatttcgcg atcgcgattg tgtttactga
     1981 tattcccgta aaagaagtcg aaaaacacgt tacaatagcg gggtactgga ttttcgtgag
     2041 acaggaagaa gatggacatt aacaacaaag cccgcattca ttgggcatgc cgccgtggta
     2101 tgcgcgaact cgatatttca atcatgccgt ttttcgaaca tgagtacgac agcttaagcg
     2161 atgacgaaaa acgcatcttt attcgtctgc tggaatgtga cgatccagac ctgtttaact
     2221 ggctgatgaa tcacggtaaa ccggccgatg cagaactgga aatgatggtc cgactcatcc
     2281 agacacggaa ccgggaacgt ggtcctgtgg caatctgatt tgcgcgtctc ctggcgcgca
     2341 cagtggcttt ccttgctgat tcatgggctg gttgccgctg ttattttact catgccctgg
     2401 ccgctcagtt acaccccgtt atggatggtg ttactttcgc tggtggtgtt tgattgcgtt
     2461 cgcagccagc ggcgtattaa tgctcgccag ggggaaattc gcttgttgat ggacgggcgt
     2521 ttgcgttggc aagggcagga gtggagcatc gtcaaagcac cgtggatgat taagagcggc
     2581 atgatgctgc gtttacgttc tgatggcggt aaacggcaac atttatggct ggcagccgac
     2641 agcatggacg aagccgaatg gcgggattta cggcggattt tgttgcaaca agagacgcaa
     2701 agataaagat gacggcaaag catatgcgct aagccgtcag tgacatcagg cgtaatgctc
     2761 tgccatttcg ttgaggattt gttcgcacca gctctgaata cgctcgtcac taaggtcata
     2821 ctggttagtt tcgtccagcg ccagccccac gaacagttgc ccatcagcaa tcaccggttt
     2881 cgggctggta aattcatatc cttccgttgg ccagtagccg acgaacttca cgcctttggt
     2941 cgagagtttg tcatgcagca taccgagcgc atcgaggaac cactcgccgt atcccagttg
     3001 atcgccaagc ccatacagcg caacaatttt accttcgagg ttcaggtcgt cgagctgatc
     3061 ccagacggct tcccagtctt cctggatttc accaaaatcc caggtcggga tacccagaat
     3121 gagcacatcg tactgctcca ttaatttcgg ggagtcgtcc ttgaggttat gtaaggtcac
     3181 cagttctggg ccgataatat cgcggatttt ttctgccgcc atttcggtgt aacaggtgct
     3241 ggaaccgtaa aaaagaccca tattcataac gtaagcttct cgattctgct gtggcttttg
     3301 cggctagtgt atcagaatcg ctttagtcac ggcacaataa ggcataatgg atcaaatgag
     3361 tgaccataag gggcgcaagt gaaacaggat ctggcacgca tcgagcagtt tcttgatgct
     3421 ctgtggctgg aaaaaaatct ggctgaaaat acgttgaacg cttaccgtcg cgatctgtca
     3481 atgatggtgg agtggttgca tcaccgcggg ttgacgctgg cgacggcgca aagtgacgat
     3541 ttgcaggcat tactggcaga acggctggag ggcgggtata aagccaccag ctcagcgcgt
     3601 ttgctgagtg cagtgcgccg attgttccag tatctttatc gcgaaaagtt tcgtgaagac
     3661 gatcccagtg cgcatctcgc ttcaccgaaa ttgccccagc gtttgccaaa agatttaagc
     3721 gaagcgcagg ttgaacgttt attacaggca ccattaattg atcagccact ggagctacgc
     3781 gataaagcca tgcttgaagt gttgtatgct accgggctgc gtgtctctga actggtcgga
     3841 ctgacaatga gtgatatcag cctgcgtcag ggcgtagtgc gggtcattgg taaaggcaac
     3901 aaagagcgcc tggtaccgtt aggtgaagag gcggtttact ggctggaaac ctatctggaa
     3961 catgggcgtc cgtggctgct gaatggtgtg tcaattgacg tgttgtttcc cagccagcgt
     4021 gcgcagcaga tgacgcgaca gaccttctgg caccgtatta aacattatgc cgtgctggcg
     4081 ggtatcgaca gcgaaaagct gtcaccgcat gtgttgcgcc acgcttttgc cactcattta
     4141 ttaaatcatg gtgcggattt acgcgtggtg cagatgctac tgggccacag cgatctttcc
     4201 accacgcaaa tttatacgca tgtcgctacc gagcgtctgc ggcaacttca tcaacagcat
     4261 cacccgcggg cgtgatgtct gaaaagaacg ggaagattta tgaagaaagg ttttatgttg
     4321 tttactttgt tagcggcgtt ttcaggcttt gctcaggctg atgacgcggc aattcaacaa
     4381 acgttagcca aaatgggcat caaaagcagc gatattcagc ccgcgcccgt agctggcatg
     4441 aagacagttc tgactaacag cggtgtgttg tacatcaccg atgatggtaa acatatcatt
     4501 caggggccaa tgtatgacgt tagcggcacg gctccggtca atgtcaccaa taagatgctg
     4561 ttaaagcagt tgaatgcgct tgaaaaagag atgatcgttt ataaagcgcc gcaggaaaaa
     4621 cacgtcatca ccgtgtttac tgatattacc tgtggttact gccacaaact gcatgagcaa
     4681 atggcagact acaacgcgct ggggatcacc gtgcgttatc ttgctttccc gcgccagggg
     4741 ctggatagcg atgctgagaa agaaatgaaa gctatctggt gtgcgaaaga caaaaacaaa
     4801 gcgtttgatg atgtgatggc aggtaaaagc gtcgcaccag ccagttgcga cgtggatatt
     4861 gccgaccatt acgcacttgg cgtccagctt ggcgttagtg gtactccggc agttgtgctg
     4921 agcaatggca cacttgttcc gggttaccag ccgccgaaag acatgaaaga attcctcgac
     4981 gaacaccaaa aaatgaccag cggtaaataa ttcgcgtgaa acaacagata caacttcgtc
     5041 gccgtgaagt cgatgaaacg gcagacttgc ccgctgaatt gcctcccttg ctgcgccgtt
     5101 tatacgccag ccggggagta cgcagtgcgc aagaactgga acgcagtgtt aaaggtatgc
     5161 tgccctggca gcaactgagc ggcgtcgaaa aggccgttga gatcctttac aacgcttttc
     5221 gcgaaggaac gcggattatt gtggtcggtg atttcgacgc tgacggtgcc accagcacgg
     5281 ctctaagcgt gctggcgatg cgctcgcttg gttgcagcaa tatcgactac ctggtaccaa
     5341 accgtttcga agacggttac ggcttaagcc cggaagtggt cgatcaggcc catgcccgtg
     5401 gcgcgcagtt aattgtcacg gtggataacg gtatttcctc ccatgcgggg gttgagcacg
     5461 ctcgctcgtt gggcatcccg gttattgtta ccgatcacca tttgccaggc gacacattac
     5521 ccgcagcgga agcgatcatt aaccctaact tgcgcgactg taatttcccg tcgaaatcac
     5581 tggcaggcgt gggtgtggcg ttttatctga tgctggcgct gcgtaccttt ttgcgcgatc
     5641 agggctggtt tgatgagcgt aacatcgcaa ttcctaacct ggcagaactg ctggatctgg
     5701 tcgcgctggg gacagtagcg gacgtcgtgc cgctagacgc taataatcgc attctgacct
     5761 ggcaggggat gagtcgcatc cgtgccggaa agtgccgtcc ggggattaaa gcgctgctgg
     5821 aagtggcaaa ccgtgatgca caaaaactcg ccgccagcga tttaggtttt gcgctggggc
     5881 cacgtctcaa tgctgccggg cgactggacg atatgtccgt cggtgtggcg ctgttgttgt
     5941 gcgacaacat cggcgaagcg cgcgtgctgg caaatgaact cgatgcgcta aaccagacgc
     6001 gaaaagagat cgaacaagga atgcaaattg aagccctgac cctgtgcgag aaactggagc
     6061 gcagccgtga cacgctaccc ggcgggctgg caatgtatca ccccgaatgg catcagggcg
     6121 ttgtcggtat tctggcttcg cgcatcaaag agcgttttca ccgtccggtt atcgcctttg
     6181 cgccagcagg tgacggtacg ctgaaaggtt ccggtcgctc cattcagggg ctacatatgc
     6241 gtgatgcgct ggagcgatta gacacactct accccggtat gatgctcaag tttggcggtc
     6301 atgcgatggc ggcgggtttg tcgctggaag aggataaatt cgaactcttt caacaacggt
     6361 ttggcgaact ggttactgag tggctggacc cttcgctatt gcaaggcgaa gtggtgtcag
     6421 acggtccgtt aagcccggcc gaaatgacca tggaagtggc gcagctgctg cgcgatgctg
     6481 gcccgtgggg gcagatgttc ccggagccgc tgtttgacgg tcatttccgt ctgctgcaac
     6541 agcggctggt gggcgaacgt catttgaagg tgatggtcga accggtcggc ggcggtccac
     6601 tgctggatgg tattgctttt aatgtcgata ccgccctctg gccggataac ggcgtgcgtg
     6661 aagtgcaact ggcttataag ctcgatatca acgagtttcg cggcaaccgc agcctgcaaa
     6721 ttatcatcga caatatctgg ccaatttagc gtcatcttct ctataaaaaa gagcgtggat
     6781 tgggtacaat cccgctctta tcaccgcatt ttgactagct caataaaaga aatcagacca
     6841 tgtttgaaat taatccggta aataatcgca ttcaggacct cacggaacgc tccgacgttc
     6901 ttagggggta tctttgacta cgacgccaag aaagagcgtc tggaagaagt aaacgccgag
     6961 ctggaacagc cggatgtctg gaacgaaccc gaacgcgcac aggcgctggg taaagagcgt
     7021 tcctccctcg aagccgttgt cgacaccctc gaccaaatga aacaggggct ggaagatgtt
     7081 tctggtctgc tggaactggc tgtagaagct gacgacgaag aaacctttaa cgaagccgtt
     7141 gctgaactcg acgccctgga agaaaaactg gcgcagcttg agttccgccg tatgttctct
     7201 ggcgaatatg acagcgccga ctgctacctc gatattcagg cggggtctgg cggtacagaa
     7261 gcacaggact gggcgagcat gcttgagcgt atgtatctgc gctgggcaga atcgcgtggt
     7321 ttcaaaactg aaatcatcga agagtcggaa ggtgaagtgg cgggtattaa atccgtgact
     7381 atcaaaatct ccggcgatta cgcttacggc tggctgcgta cagaaaccgg cgttcaccgc
     7441 ctggtgcgta aaagcccgtt tgactccggc ggtcgtcgcc atacgtcgtt cagctccgcg
     7501 tttgtttacc cggaagtcga tgacgatatt gatatcgaaa tcaacccggc ggatctgcgc
     7561 attgacgttt atcgcgcgtc cggcgcgggc ggtcagcacg ttaaccgtac cgaatctgcg
     7621 gtgcgtatta ctcacatccc aaccgggatc gtgacccagt gccagaacga ccgttcccag
     7681 cacaagaaca aagaccaggc catgaagcag atgaaagcga agctttatga actggagatg
     7741 cagaagaaaa atgccgagaa acaggcgatg gaagataaca aatccgatat cggctggggc
     7801 agccagattc gttcttatgt ccttgatgac tcccgcatta aagatctgcg cactggggta
     7861 gaaacccgca acacgcaggc cgtgctggac ggcagcctgg atcaatttat cgaagcaagt
     7921 ttgaaagcag ggttatgagg aaccaacatg tctgaacaac acgcacaggg cgctgacgcg
     7981 gtagtcgatc ttaacaatga actgaaaacg cgtcgtgaga agctggcgaa cctgcgcgag
     8041 caggggattg ccttcccgaa cgatttccgt cgcgatcata cctctgacca attgcacgca
     8101 gaattcggcg gtaaagagaa cgaagaactg gaagcgctga atatcgaagt cgccgttgct
     8161 ggccgcatga tgacccgccg tattatgggt aaagcgtctt tcgttaccct gcaggatgtg
     8221 ggtggccgca ttcagctgta cgttgcccgt gacgatctgc cggaaggcgt ttacaacgag
     8281 cagttcaaaa aatgggacct cggcgacatc ctcggcgcga aaggtaaact gttcaaaacc
     8341 aaaaccggcg aactgtctat ccactgtacc gagctgcgtc tgctgaccaa agcactgcgt
     8401 ccgctgccgg ataaattcca cggcttgcag gatcaggaag cgcgctatcg tcagcgttat
     8461 ctggatctca tctctaacga tgaatcccgt aacaccttta aagtgcgctc gcagatcctc
     8521 tctggtattc gccagttcat ggtgaaccgc ggctttatgg aagttgaaac gccgatgatg
     8581 caggtgatcc caggcggtgc cgctgcgcgt ccgtttatca cccaccataa cgcgctggat
     8641 ctcgacatgt acctgcgtat cgcgccggaa ctgtacctca agcgtctggt ggtcggtggc
     8701 ttcgagcgtg tattcgaaat caaccgtaac ttccgtaacg aaggtatctc cgtacgtcat
     8761 aacccagagt tcaccatgat ggaactctac atggcttacg cagattacaa agatctgatc
     8821 gagctgaccg aatcgctgtt ccgtactctg gcacaggata ttctcggtaa gacggaagtg
     8881 acctacggcg acgtgacgct ggacttcggt aaaccgttcg aaaaactgac catgcgtgaa
     8941 gcgatcaaga aatatcgccc ggaaaccgac atggcggatc tggacaactt cgactctgcg
     9001 aaagcgattg ctgaatctat cggtatccac gttgagaaga gctggggtct gggccgtatc
     9061 gttaccgaga tcttcgaaga agtggcagaa gcacatctga ttcagccgac cttcattact
     9121 gaatatccgg cagaagtttc tccgttggcg cgtcgtaacg acgttaaccc ggaaatcaca
     9181 gaccgctttg agttcttcat tggtggtcgt gaaatcggta acggctttag cgaactgaat
     9241 gacgcggaag atcaggcgca gcgcttcctg gatcaggttg ccgcgaaaga tgcaggtgac
     9301 gacgaagcga tgttctacga tgaagattac gtcaccgcac tggaacatgg cttaccgccg
     9361 acagcaggtc tggggattgg tatcgaccgt atggtaatgc tgttcaccaa cagccatacc
     9421 atccgcgacg ttattctgtt cccggcgatg cgtccggtga aataagcatt acgttatgct
     9481 cacaaccccg gcaaatgtcg gggtttttta tttaagctgg gtaaatgcag ataatcgtat
     9541 tctggcttcg cgatttgtcg cctgcatcac catccacgga ctgaacgccc acggcgtggc
     9601 atcaataccg cgtaatacat cagctaaatc acaccattga taatccatca cttcatcatc
     9661 gttgatctgt aacgcactgg tggtgcgtgc ggcaaatacc ggacacactt cattttccac
     9721 aatgccattc ggatcggtgg cgcgatagcg aaagtcagga tagatagatt caggaggcgt
     9781 aatttccacg ccaagctcat aacggcaacg gcggatcacc gcgtcttcgt tgctttctcc
     9841 cagttgtggg tgcccacaaa ccgggttagt ccacacgcca ggccatgctt ttttgctcag
     9901 tgcgcggcgg gtaactaata attgtccttt ggcattaaac agccaactgg agaacgcgag
     9961 atgtaagcgg gtgtctgccg tgtgtgcggc atacttttcc agcgtacccg tgggaactcc
    10021 ctgtgcattc aataaaatga cgtgttccgt ttgcataatt tctcacatgt aattctgatc
    10081 actcatcatt atagataatt gaagagtgaa atgattaacc aagtgagcgt ttatcgacaa
    10141 ccgcccgttt tgagcggatg tcgacaggta aaaaccattt aaaaaagtag catcatatat
    10201 ttgaagcgac tttcaatggc cattgcaaca cagtcagaaa tgacaggaat agagagagta
    10261 aaaagcaaca gcagactgaa tatagcgcgc attaaaaaat tcatattatt ctccatgctc
    10321 atttttcttc agcttgcgca tcagaagcca catacgtact gtcctgactt ttaaatcttt
    10381 atcagagacg gattccttca ccaggtcagc atgatgctgg tagccattaa agaagacatt
    10441 cagcacaacg gcgctaaggg ttgcgagcat aatgccgcta tgcagcagcg gttgcagtac
    10501 ggccggtaac ttagaaaaga aatcgtgaga gagcgtcgga gtcatgccaa cgccgagact
    10561 gatcgccaca atatagaggt tgtaacggtt ggtggtgtag ttacagcgcg acagaattcg
    10621 aatccctgtc gccagtacca tgccgaacat caccagccca gcgccgccca gcacaaattg
    10681 cggaatggag gctaccagca ccgccatttt tggcaccatg ccaaacagga ttaatataat
    10741 tcccgaagaa atacacaccc agcggctatg aacgcgcgtc acgctaacca ggccaacgtt
    10801 ttgagaaaat gacgtatggg ggaagctgtt aaacgtgccg cctatcattg tccctacgcc
    10861 atcgacacgc agcccgcgaa taatatcgtg tgaagagagt ttacgaccaa ctatttcacc
    10921 cagtgccagg aacatcccca tcgactcgat aaacacgatg attaacacgg cagtcatggt
    10981 cagaatggaa acgggatcaa aaatcggcat accaaacgac atcggcgtaa caatcgcaaa
    11041 ccatgaagca tcatgtagcc cggataaatt gacttcattc atcatccacg aaagtaaaaa
    11101 gccaaataca atccccagta atacggcgac gttggacata aatcctttcg catagcgagt
    11161 aatgagcaag ataaaaatta agacggcaaa ggagatacct aaataaacgg gattaccata
    11221 ttgcggattc cctttacctc cggcggccca gtcaataccc acctgaatga tgctaagccc
    11281 gatagaagta ataaccacac cggtaaccag tggcggaaat aaaggcatca agcgaccgat
    11341 aagtggcgct aataatgtgg tgataaaacc cgcggcgata gtggcaccaa atatccccag
    11401 caggccgata tccgggttca tcccaatggc tatcattggt gttacagcag caaaggtcac
    11461 cgacataatc accggcaggc ggatccccat aaagcggccg ataccgatac attgcaataa
    11521 tgtgacgatc ccgcagcaaa agagatccga gctaatgagc atcgcaatag catcttttga
    11581 gaggcccagt cggtcaccaa tcataagagg aacagcgact gcacctgcgt acatgacaag
    11641 gacgtgttgc agaccgagta tgatcagctt tcctggtgat aatatgcggt caacctcatc
    11701 agttgggcgg tcttgcccag aagatgaggg aagttgggaa tctatggcgc tcatcagtgt
    11761 ttctcctttc gcccgccaga gtaaaaagca accgtttacg ttaatgaaac tgtctaaaca
    11821 catgctcgtg gagtgccagt ttttgcgagg aaggggagtt agagacagga attgcggagg
    11881 caatcacata atcgtgctta aaccacgaaa atgagaagag aacctgacaa tggaattatc
    11941 tgtaattttc tcatattgat atttcattaa tcattctggc atgtcgcact ctcgcattta
    12001 atcgttttta tctggatagc gctcttttga tcggcaatgt tgaaataatg aaagcggcat
    12061 agattgaggg gcaataattt gaactcagtc gcaaaattat aattaaaacg gtcatgctac
    12121 ctaatatatg cccgtttatt gcatcgcgaa atttgagaag acataactca ttaaatgaaa
    12181 gggatgcaaa tgaataagtt tatcgctgct gaagctgcgg aatgtatagg ctgtcatgct
    12241 tgtgaaattg cctgtgcggt ggcacacaat caagaaaact ggccgctgag tcacagtgac
    12301 tttcgaccac gtatccacat tgtagggaaa ggccaggctg cgaatccggt ggcctgccat
    12361 cactgcaaca atgccccttg cgttacggct tgtccggtta atgctctgac tttccagtcc
    12421 gatagcgtac aactggacga gcaaaaatgt attggttgta aaagatgcgc aatcgcttgc
    12481 ccctttggcg tcgttgagat ggtcgatacg attgcgcaga aatgcgacct ttgtaaccag
    12541 cgcagttccg gcacgcaagc ctgtattgaa gtttgcccaa cacaggcgtt acgactgatg
    12601 gacgataaag ggttacagca gataaaggtg gcccgccagc gcaaaacggc agcaggaaaa
    12661 gcgtcatcag acgctcagcc atctcgcagt gcagcgttgc tccccgttaa ctcgcgtaaa
    12721 ggcgcagata aaatttcagc aagtgaacgg aaaaaccatt ttggcgaaat ctattgcggg
    12781 ctggatccac aacaagcgac ttatgagagt gaccgctgtg tttattgtgc cgaaaaagct
    12841 aactgcaact ggcattgccc gctgcataac gctattccgg attacatccg tctggtacag
    12901 gagggaaaga ttattgaagc ggcagaactt tgccaccaga ccagttcctt accagaaatc
    12961 tgcggcaggg tatgtccaca agaccgtctt tgtgaaggcg catgtacttt gaaagatcac
    13021 tctggcgcag tctctatcgg taatctggaa cgctacatca ccgataccgc gctggcgatg
    13081 ggctggcgtc ccgatgtcag caaagttgtt ccccgtatcg agaaagtggc ggtgattggc
    13141 gctgggcctg cagggttagg gtgtgctgat attctggcgc gcgcaggagt tcaggtcgat
    13201 gtctttgatc gccatccaga aattggcggt atgctgactt ttggcattcc tcctttcaaa
    13261 ctcgataaaa cggtattaag ccagcggcga gagatattca ccgcaatggg aatcgacttt
    13321 catcttaact gtgaaattgg ctgcgatatt acctttagcg atttaacttc tgaatatgat
    13381 gcagttttca tcggcgtggg gacttacggg atgatgcgag ctgatctgcc gcatgaagat
    13441 gcgcccggtg tcattcaggc tctaccgttc ttgactgccc atacccgcca gctcatgggg
    13501 ttgccggagt ctgaagagta tccgctgacg gacgtggaag gtaagcgagt cgtggtattg
    13561 ggcggtggcg atacgacaat ggattgtttg cggacttcca tccgcctcaa tgccgccagc
    13621 gtgacctgcg cgtatcgtcg tgatgaagtc agtatgccgg gctcgcgcaa agaggtggtc
    13681 aatgcgcgcg aggaaggtgt cgagtttcaa ttcaatgttc agccgcaata tatcgcttgt
    13741 gacgaagatg ggcgtttaac tgcggtgggc ctgattcgta ccgccatggg tgagccgggg
    13801 ccggatggtc gccgtcgtcc tcgtccggtt gcgggttcag agtttgaatt gcccgccgat
    13861 gttctcatta tggcctttgg tttccaggcg catgccatgc cgtggttgca gggcagcgga
    13921 attaaactcg ataaatgggg cctgattcaa accggcgacg ttgggtattt acctacccag
    13981 acgcatctga aaaaagtctt tgctggtggg gatgcagttc atggcgcgga tctggttgtc
    14041 actgccatgg ccgcaggaag gcaggcggcg cgcgatatgt taactctgtt tgatacgaag
    14101 gcatcgtgat gaaatcgtta attatcgtta atccggctga ctgtattggc tgccgtactt
    14161 gtgaagtggc ctgtgtggtc gctcaccctt cagaacagga gttgaatgcc gatatctttt
    14221 taccccggct gaaggtacag cgactggact gcatcagtgc gccggtgatg tgccaccaat
    14281 gtgaaaacgc cccttgtgtt ggcgcttgcc ccgtgggggc gctgacgatg ggcgagcagg
    14341 tggtgcagac caattctgcc cgttgtattg gctgtcagag ttgcgtcagt gcgtgcccgt
    14401 ttggaatgat caccattcag tcgttgctgg gggatacccg gcaacaaatc gtgaaatgcg
    14461 atctctgtga acagcgggaa gaggggcctg cctgcgttga atcttgccca acacaggcgc
    14521 tgcagttgct tactgaaaga gaactcaggc gagtccgcca gcagcgtatt gttgccagcg
    14581 gtgagaatct tctctgaaag aaatctccgc gctgaaaggc gcggagaatc tattagattg
    14641 cccaaccacc cgcgtaaaat gcgaccagtg cggcagtaat gatcaccgta ccaatattca
    14701 gcttttgcca ttcgcgtgca aagacgcggc ctacgaccag tgtcacaaag cccagcataa
    14761 taccggtaac gatattacaa gtcagaacga tgaacacggc acacaccagg ccagccatag
    14821 cgtcaataaa atcattgaaa tccagcttcg agacgttact taacatcagc aaacctacgt
    14881 acatcagagc gggtgcagtg gcgtaaccag ggatcaaaaa tgacagcggt gataagaaca
    14941 gaatcagcag gaataacgcc cccactacgg ttgcggttaa ccctgtttta cccccggcgg
    15001 cggttcctgc cgctgattcg atataaaccg ctgcgggcgc tgcgcccacc aggccggaga
    15061 atattgaact tacagagtca ctggtcaggg ctttgccgcc gttgatgatc tggttgtctt
    15121 tatccaacaa attcgcctga ccggcgacgg cacggatggt gccagtagcg tcgaacactg
    15181 cggtcatcac caatgccagt acactcggaa gtacagttgg ctggagtgcg cccataatat
    15241 cgaggctgaa aatcagagac ttaccatctt cgccagtcag gcttggcatc gccaccagac
    15301 cgtggtattt caccgctggg tcaaagatta agccgatgat cgaaattgca ataatcacca
    15361 acaagatccc gccgggtacg cgacacttct ccagaccgaa gatgaccgcc agccccagca
    15421 agctcatcat caccgggaag gaggtaaacg caccgagcgc cactggcaag ccttcaatcg
    15481 ggtttttgat aaccataccc acaccgttag cagcaatcag cagcagaaac agcccgatac
    15541 cgatacctgt accgtgagcg atacccatcg gcaaattacg taagatccag gtacgcacac
    15601 cggttacgga aatggcggtg aagatgacgc ccatcagaaa taccgcgccc agtgcgacag
    15661 gaacgctaat ttgttgcccg agtaccagac tgaatgcggt aaacgccgtc aaggaaatcg
    15721 cgcaaccaat cgccattggc aaattagccc ataaccccat cagcaacgag ccgaagcccg
    15781 cgaccagaca ggtggcaaca aacacagctg cgggaggaaa acctgctttg cccagcattc
    15841 ccggaacgac gataacggaa taaaccatgg ccagaaaggt cgttaagcca gccagtactt
    15901 cctgacgaac agtactgcca cgagcggtaa ttttaaaata attatccagc gcgccctgtg
    15961 gctttggtgc gtccggtgtt tgtaggatgt ctccagacat aatgatgtcc tcacgaggat
    16021 gttaaagttt tattattagt tgcgttcgta caccagacga ccatcaacgt aggtgcggta
    16081 gatcgaacgg tcatcgccca acgtcatcat cacgaacaat ttgtcgacta aagaaacaga
    16141 gttgtcatag cgcagctgct gtagcggagt ggcggtgggt tccatcacca cgaaatcagc
    16201 ctctttgcca ggtaaaaagt tgccaatcaa atcgtcaagg cccagagatt tcgctccgcc
    16261 gagcgtggcc aggtaaaacg cttcatatgc cgagaggcga tagccttgta attgcaacac
    16321 tttgtaggct tcgttcagcg tttgcagcat gttgaaagtg gttccggcac cgatatccgt
    16381 tcccatgccc actttaactt tcttctgcca tgcttttttc aagttgaata acccgctgcc
    16441 caggtaaagg ttggaggtcg gacagaaagc aatgctggat ttggtttcgc tgagacgatc
    16501 ccactctttt tcttcgagat ggacgcagtg agcaaagaca cagtttttac cggtcaggcc
    16561 gtactgatgg taaacatcca gataaccatc atggtcagga taaagcgatt tcacccaggc
    16621 aatttcatct ttgttttcac agagatgggt atgtacccac gtatccggat attcctcttt
    16681 caggcgttgc gccatcgcca tctgttcagg agatgaggta ggggcgaatc gtggcgtaat
    16741 cgcatacaac agacgaccat ttttgtgcca gcgttcgatc agttctttgc tttggtgata
    16801 gctgctttcg gcagtgtcga gcagataatc cggtgcgtta cggtccatca tcaccttacc
    16861 ggcaatcata cgcatattga tatgactggc ggcttcaaac agcgcatcaa cggattgcgg
    16921 atgaacagtg ccaaacacca gcgccgtggt ggttccgtta cgtaaaagct gcttgatgaa
    16981 gaacgccgac atttcgcggg cgtactctaa atcctcataa cgacgttcag tagggaaggt
    17041 gtgtttattc aaccactcca gcaattgctc accataggcc cccaccattt cactttgcgg
    17101 ataatggata tgtgtatcga caaagcccgg tactatcagt ttgccgcgat agtcgcgcac
    17161 gcgaatggtg tcaggaattt gatgctttcc gttttcccat tcgccaaacc attccacttt
    17221 tccctgttta atgagtaata aaccatcctc aataaaccgc agcgcagagg caatctcttc
    17281 cgggttatcg accgtacggg tgacatcaat aaaactgcct cgtaccgctt ttaacgtgtg
    17341 ttctcctgac atcattgact cctttactgt taatccatct cttccgctga ggtaatacca
    17401 ggcagaacgt tttcctgtcg gtagccacca gggaggataa tatttaataa aatcgcagtt
    17461 aacccgccag cacaaatagg gttttcaact aatacataaa tagaggctgg caatatttta
    17521 aaaatttcgg gatcgtagga gacgccaagc cctaaaccta aagaagtggc gacaataagt
    17581 gtttcacgcc gctttaatcc gttggtgatg atgatgcgaa tcccagcgat ggcaatcatg
    17641 gaaaacatca acgtcattgc gcctcccaga actgccgagg gaatggtcgt gaagaagccg
    17701 ccaatcatcg gaaataagcc gaggataacc agcattaccg cgatggttcg cccgacataa
    17761 cgtgaagcga cgccagtcat ctgaataacc ccattatttt gcgcaaacgt ggttaatggt
    17821 aatgaaccaa cagcggaggc gataacagaa accagaccat ctgccagcac gccgcctttc
    17881 agtcgggact gatactcttc cccctgaatg gggcggcggg aaaccattgc cgtggcggtg
    17941 atatcgccga ctgcttccag cacgctaagc agataaatcg tgccaaccac caggaactga
    18001 tggaagctaa aactaaagcc gtatttgaac ggatgcggga tggtgattaa cggcaaattg
    18061 cgcatactgc tgaaatccac catgcccagg cataacgatg cgatatagcc gacacatagc
    18121 ccaatggcga tccctcccat gcgtagcaac ggactgcgac agcagttaaa gccgatcacc
    18181 acaattaaaa ccaataaacc aacgccgaga tgttcgtaat tgccgaacgt accgctgctt
    18241 ttggctgcaa atcctccacc aaaatcgata atgccgactt taatcaggct taagccgatc
    18301 atcagtacca caataccgct gacggtaggc gtaataaccc gacgtaaata gggcaagata
    18361 aatgaagatc cgacaaccag aaatgcgcca acgaaggaga cgccgagaag cgacgacatg
    18421 attaactctt cgtgaaaacc gtcgcttttc atgctgctgc ccagcgcaat catgaccgta
    18481 acaaatgaaa aattgactga ctgaattgag agtaggccag aaccgacgat gccgtagcgg
    18541 tttacttgta accaggtacc aataccagag gcgatcatcg ccatagaaac aagataggcg
    18601 gttgtttcag cggaaagctg taaggccgca cccacgatta acgcgggggt taccatcgga
    18661 acgaaaattg ccaacagatg ggtaatggca ccaacgagag cctgatgaaa gggagggcga
    18721 tcctccagtt caaatataag gtcagaacct gcatggttta tatcagacat cccttcactc
    18781 cttttaaagt aaatttgatc atctga
//
LOCUS       DAZKFU020000079        18729 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZKFU020000079 DAZKFU020000000
VERSION     DAZKFU020000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.079"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..284
                     /locus_tag="ACHOQQ_RS10825"
                     /old_locus_tag="pgaptmp_002165"
     CDS             <1..284
                     /locus_tag="ACHOQQ_RS10825"
                     /old_locus_tag="pgaptmp_002165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470418.1"
                     /translation="HFVSNLAPVKYKHSIKPAKNYRSINKYITAPSPFSQISNHHYVF
                     YFFTAKSLAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
     gene            238..1902
                     /gene="asnB"
                     /locus_tag="ACHOQQ_RS10830"
                     /old_locus_tag="pgaptmp_002166"
     CDS             238..1902
                     /gene="asnB"
                     /locus_tag="ACHOQQ_RS10830"
                     /old_locus_tag="pgaptmp_002166"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGU4470419.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2282..2358
                     /locus_tag="ACHOQQ_RS10835"
                     /old_locus_tag="pgaptmp_002167"
     tRNA            2282..2358
                     /locus_tag="ACHOQQ_RS10835"
                     /old_locus_tag="pgaptmp_002167"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2316..2318,aa:Met,seq:cat)
     gene            2368..2452
                     /locus_tag="ACHOQQ_RS10840"
                     /old_locus_tag="pgaptmp_002168"
     tRNA            2368..2452
                     /locus_tag="ACHOQQ_RS10840"
                     /old_locus_tag="pgaptmp_002168"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2402..2404,aa:Leu,seq:tag)
     gene            2476..2550
                     /locus_tag="ACHOQQ_RS10845"
                     /old_locus_tag="pgaptmp_002169"
     tRNA            2476..2550
                     /locus_tag="ACHOQQ_RS10845"
                     /old_locus_tag="pgaptmp_002169"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2508..2510,aa:Gln,seq:ttg)
     gene            2585..2659
                     /locus_tag="ACHOQQ_RS10850"
                     /old_locus_tag="pgaptmp_002170"
     tRNA            2585..2659
                     /locus_tag="ACHOQQ_RS10850"
                     /old_locus_tag="pgaptmp_002170"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2617..2619,aa:Gln,seq:ttg)
     gene            2675..2751
                     /locus_tag="ACHOQQ_RS10855"
                     /old_locus_tag="pgaptmp_002171"
     tRNA            2675..2751
                     /locus_tag="ACHOQQ_RS10855"
                     /old_locus_tag="pgaptmp_002171"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2709..2711,aa:Met,seq:cat)
     gene            2800..2874
                     /locus_tag="ACHOQQ_RS10860"
                     /old_locus_tag="pgaptmp_002172"
     tRNA            2800..2874
                     /locus_tag="ACHOQQ_RS10860"
                     /old_locus_tag="pgaptmp_002172"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2832..2834,aa:Gln,seq:ctg)
     gene            2912..2986
                     /locus_tag="ACHOQQ_RS10865"
                     /old_locus_tag="pgaptmp_002173"
     tRNA            2912..2986
                     /locus_tag="ACHOQQ_RS10865"
                     /old_locus_tag="pgaptmp_002173"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2944..2946,aa:Gln,seq:ctg)
     gene            complement(3140..4315)
                     /gene="ubiF"
                     /locus_tag="ACHOQQ_RS10870"
                     /old_locus_tag="pgaptmp_002174"
     CDS             complement(3140..4315)
                     /gene="ubiF"
                     /locus_tag="ACHOQQ_RS10870"
                     /old_locus_tag="pgaptmp_002174"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGU4470420.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRSKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4461..5885
                     /gene="miaB"
                     /locus_tag="ACHOQQ_RS10875"
                     /old_locus_tag="pgaptmp_002175"
     CDS             4461..5885
                     /gene="miaB"
                     /locus_tag="ACHOQQ_RS10875"
                     /old_locus_tag="pgaptmp_002175"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGU4470421.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6038..7078
                     /locus_tag="ACHOQQ_RS10880"
                     /old_locus_tag="pgaptmp_002176"
     CDS             6038..7078
                     /locus_tag="ACHOQQ_RS10880"
                     /old_locus_tag="pgaptmp_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGU4470422.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7075..7542
                     /gene="ybeY"
                     /locus_tag="ACHOQQ_RS10885"
                     /old_locus_tag="pgaptmp_002177"
     CDS             7075..7542
                     /gene="ybeY"
                     /locus_tag="ACHOQQ_RS10885"
                     /old_locus_tag="pgaptmp_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGU4470423.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7632..8510
                     /gene="corC"
                     /locus_tag="ACHOQQ_RS10890"
                     /old_locus_tag="pgaptmp_002178"
     CDS             7632..8510
                     /gene="corC"
                     /locus_tag="ACHOQQ_RS10890"
                     /old_locus_tag="pgaptmp_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGU4470424.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8535..10073
                     /gene="lnt"
                     /locus_tag="ACHOQQ_RS10895"
                     /old_locus_tag="pgaptmp_002179"
     CDS             8535..10073
                     /gene="lnt"
                     /locus_tag="ACHOQQ_RS10895"
                     /old_locus_tag="pgaptmp_002179"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGU4470425.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWSKTTWLRVAIVAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSTLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            10470..11378
                     /gene="gltI"
                     /locus_tag="ACHOQQ_RS10900"
                     /old_locus_tag="pgaptmp_002180"
     CDS             10470..11378
                     /gene="gltI"
                     /locus_tag="ACHOQQ_RS10900"
                     /old_locus_tag="pgaptmp_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGU4470426.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            11548..12288
                     /gene="gltJ"
                     /locus_tag="ACHOQQ_RS10905"
                     /old_locus_tag="pgaptmp_002181"
     CDS             11548..12288
                     /gene="gltJ"
                     /locus_tag="ACHOQQ_RS10905"
                     /old_locus_tag="pgaptmp_002181"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGU4470427.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            12288..12962
                     /gene="gltK"
                     /locus_tag="ACHOQQ_RS10910"
                     /old_locus_tag="pgaptmp_002182"
     CDS             12288..12962
                     /gene="gltK"
                     /locus_tag="ACHOQQ_RS10910"
                     /old_locus_tag="pgaptmp_002182"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGU4470428.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12962..13687
                     /gene="gltL"
                     /locus_tag="ACHOQQ_RS10915"
                     /old_locus_tag="pgaptmp_002183"
     CDS             12962..13687
                     /gene="gltL"
                     /locus_tag="ACHOQQ_RS10915"
                     /old_locus_tag="pgaptmp_002183"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGU4470429.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            13805..14740
                     /gene="rihA"
                     /locus_tag="ACHOQQ_RS10920"
                     /old_locus_tag="pgaptmp_002184"
     CDS             13805..14740
                     /gene="rihA"
                     /locus_tag="ACHOQQ_RS10920"
                     /old_locus_tag="pgaptmp_002184"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGU4470430.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQKC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHGEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            14824..16494
                     /gene="hscC"
                     /locus_tag="ACHOQQ_RS10925"
                     /old_locus_tag="pgaptmp_002185"
     CDS             14824..16494
                     /gene="hscC"
                     /locus_tag="ACHOQQ_RS10925"
                     /old_locus_tag="pgaptmp_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGU4470431.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHTSAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIEIP"
     gene            complement(16554..18005)
                     /gene="djlC"
                     /locus_tag="ACHOQQ_RS10930"
                     /old_locus_tag="pgaptmp_002186"
     CDS             complement(16554..18005)
                     /gene="djlC"
                     /locus_tag="ACHOQQ_RS10930"
                     /old_locus_tag="pgaptmp_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HGU4470432.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDDATEHEILRVFRALLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSVGMDEEEREAFLEAI
                     QAGDCFDFLGLLEYPVALQNQTVEYYFALERCCHYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWGSYPSTQADNLLLQWSAQHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(18002..18709)
                     /locus_tag="ACHOQQ_RS10935"
                     /old_locus_tag="pgaptmp_002187"
     CDS             complement(18002..18709)
                     /locus_tag="ACHOQQ_RS10935"
                     /old_locus_tag="pgaptmp_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4470433.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         4605 a         4830 c         4759 g         4535 t
ORIGIN      
        1 cgcactttgt cagcaatttg gcccccgtaa aatataaaca ttcaataaaa cccgccaaaa
       61 attatcgatc cattaataaa tacatcaccg caccctcccc tttttcacaa atcagcaatc
      121 accattacgt tttttatttt ttcaccgcca aatcgcttgc gcacagtgct gctttgcggc
      181 atttttttaa acaagcaaac acaacaagca acaaatacca ggttaacgga gaaggttatg
      241 tgttcaattt ttggcgtatt cgatatcaaa acagacgcag ttgagctgcg taagaaagca
      301 ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact ggtccggtat ttatgccagt
      361 gataacgcca ttctcgccca cgaacgtctg tcaattgttg acgttaacgc gggggcgcaa
      421 cctctctaca accaacaaaa aacccacgta ctggcggtaa acggtgaaat ctacaatcac
      481 caggcattgc gcgctgaata cggcgatcgt tatcagttcc agacagggtc tgactgtgaa
      541 gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc tcgacgactt gcagggcatg
      601 tttgcctttg ccctgtacga cagcgaaaaa gatgcctacc tgattggtcg cgaccatctg
      661 gggatcatcc cactgtatat gggctatgac gaacatggtc agctgtatgt ggcctcagaa
      721 atgaaagccc tcgtgccggt atgccgcacg attaaagagt tcccggcggg gagctatttg
      781 tggagtcagg acggcgaaat ccgttcttat tatcatcgcg actggttcga ctacgatgcg
      841 gtgaaagata atgtcaccga caaaaacgag ctgcgtcagg ctctggaaga ttccgttaaa
      901 agccatctga tgtctgatgt gccttacggt gtgctgcttt ctggtggtct ggattcctca
      961 attatttccg ctatcaccaa gaaatacgca gcccgtcgcg tggaagatca ggaacgctct
     1021 gaagcctggt ggccgcagtt acactccttt gctgtaggtc tgccgggttc accggatctg
     1081 aaagcagccc aggaagtggc aaaccatctg ggcactgtgc atcatgaaat tcacttcact
     1141 gtacaggaag gtctggacgc catccgcgac gtgatttacc acatcgaaac ctatgatgtg
     1201 accactattc gcgcttcaac accgatgtat ttaatgtcgc gtaagatcaa agcgatgggc
     1261 attaaaatgg tgctgtccgg tgaaggttct gatgaagtgt ttggcggtta tctttacttc
     1321 cacaaagcac cgaatgccaa agaactgcat gaagagacgg tgcgtaaact gctggccctg
     1381 catatgtatg actgcgcgcg tgccaacaaa gcgatgtcag cctggggcgt ggaagcacgc
     1441 gttccgttcc tcgacaagaa attcctcgac gtggcgatgc gcattaaccc gcaggataaa
     1501 atgtgcggta acggcaagat ggaaaaacac atcctgcgtg aatgttttga gtcatacctg
     1561 cccgcaagcg tggcctggcg gcagaaagag cagttctccg atggcgtcgg ttacagttgg
     1621 atcgacaccc tgaaagaagt ggcggcgcag caggtttctg atcaacagct ggaaactgcc
     1681 cgcttccgct tcccgtacaa cacaccgacc tcaaaagaag cgtatctgta ccgggaaatc
     1741 tttgaagaac tgttcccgct tccaagcgcc gctgagtgcg tgccgggcgg tccttccgtc
     1801 gcgtgttctt cagctaaagc gatcgaatgg gatgaagcgt tcaaaaaaat ggacgatccg
     1861 tctggtcgcg cggttggtgt tcaccagtcg gcgtataagt aagaaatgcg aaacagcccc
     1921 ggtgaaatcc ggggctgttt cagttattga ggtgcttgtt gattctgata tgcagtaagg
     1981 aaatgttccg ggtttgccac atcactgttt gacaactcat cactgtaggc cagtatttca
     2041 tagattaatt tcccaccact attgttaaat tccgaatttc atcctgtatg acaaaagaat
     2101 ggctgtaggg tgggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg
     2161 ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
     2221 gcaaggctct atacgcataa tgcgccccgc aacgccgata aggtatcgcg aaaaaaaaga
     2281 tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga
     2341 atcccgtcgt agccaccatc tttttttgcg ggagtggcga aattggtaga cgcaccagat
     2401 ttaggttctg gcgccgcaag gtgtgcgagt tcaagtctcg cctcccgcac cattcaccag
     2461 aaagcgttgt acggatgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
     2521 tccctggttc gaatccaggt accccagcca tcttcttcga gtaagcggtt caccgcccgg
     2581 ttattggggt atcgccaagc ggtaaggcac cggtttttga taccggcatt ccctggttcg
     2641 aatccaggta ccccagccat cgaagaaaca atctggctac gtagctcagt tggttagagc
     2701 acatcactca taatgatggg gtcacaggtt cgaatcccgt cgtagccacc aaattctgaa
     2761 tgtgtcgaat atgttcgaca aattcaaaaa ccaatttgtt ggggtatcgc caagcggtaa
     2821 ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca gccaatttat
     2881 tcaagacgct taccttgtaa gtgcacccag ttggggtatc gccaagcggt aaggcaccgg
     2941 attctgattc cggcattccg aggttcgaat cctcgtaccc cagccacatt aaaaaagctc
     3001 gcttcggcga gctttttgct tttctgcgtt cactcaatgt cgaatgcgat gttgacacgt
     3061 cttatccttc aatgtcggat gcgacgctgc cgcgtcttat ccgacctacg gttggcacgc
     3121 atccggcaat gttgtaaggc tacaacccta acgcatattt cagcgcctga cgtttcaaca
     3181 cgccagcacg ctccgccgcc atcaaaccaa gattacgcac aaaacgcagc ggcggcagat
     3241 tattactgaa tccggcataa aacagatcca taccgctctg cataatgaag ttatccgcca
     3301 tgcgccgcat ctggtaacgc ttgaggacag gataactggc ccacgcttcg ccgtagctgc
     3361 gggcattcac cagaacatca atcagggcat cgacatcacg ataaccaaga ttcaccccct
     3421 gccccgccag cggatggatg gtatgcgcgg catcgcccac cagcgcaagc cctggctgca
     3481 catactgcaa cgcatggcga cgcgtcagcg gaaacgcacc agcggcaagc ggtgtaacgt
     3541 aacccagacg cgacgggaaa tgcttcgcga tttccgcctg gagctgtgcc atattcatat
     3601 tctgcaactg gcgaatacgc gccggagagt cataccacac cagcgatgcc cagttatcaa
     3661 acaacggcag aaacgcacgc ggtccgtccg gagtaaattg ctgccaggtg ctatcgccgg
     3721 gatcgttctc gcactggacg ctaatcaaca tgcacgactg cgcatactgc catgcatgaa
     3781 cgccaattcc cgccatctgc cgcacctgcg aatttgcgcc gtcggcacca atcaccagct
     3841 tcgagcgaat cacttcaccg cctttcagct ccagctcctg aagatcatta tctcgatgta
     3901 atgcaatcag cgagcctggc acacgtaacg ttactttcgg atgcgcttcc agcgcctgcc
     3961 acaacgcctg ttgcaggaca gtgttttcca ccatatagcc aagcagcggt agcttaagtt
     4021 cagcggcgtc aaacaccaca tgcgccgttt cccactccca cgtttccagt ctgcggtaag
     4081 gatggcaacg catagcctgt actgcatccc agacccctaa ccctttaagc aatgataccg
     4141 aagccgcgct gatcgccgag atccgcacgt ccggttggct atcagcgaca aacggtgcag
     4201 gttctgcgtg ctcgatcacc gttaccgtaa atccgtgctg tgccagcccc agcgccagtg
     4261 cgccgccgac cattcctccg ccgacaatgg caatttccgt tggttgattt gtcatggtca
     4321 atcatcctgt tagcaatatg ctttaagttt acaggatttt tacccccaag ggctgataac
     4381 gctcatactg gtcacaacgg cagcaaagca ttacactatg cgccctgcat tcctggctac
     4441 tatttcgcaa gagcaagtcg atgaccaaaa aactccatat taaaacctgg ggctgtcaga
     4501 tgaacgagta cgattcatcg aagatggccg atctgctgga tgccacccac ggctatcaac
     4561 tgaccgacgt ggcggaagaa gcggatgtgc tgctgctgaa cacctgctca atccgcgaga
     4621 aggctcagga aaaagtcttc catcagttgg gtcgctggaa actgttaaaa gagaagaatc
     4681 cagacctgat tatcggcgtc ggtggctgcg tggcatcgca agaaggcgag cacattcgcc
     4741 agcgcgccca ctatgtcgat attatttttg ggccgcaaac gctgcaccgt ctgccggaga
     4801 tgatcaactc cgtgcgcggc gaccgcagcc cggttgtaga tatcagcttc ccggaaatcg
     4861 agaagtttga ccgtctaccg gaaccgcgcg ccgaagggcc gaccgcgttt gtctccatca
     4921 tggaaggctg caataaatat tgcacctact gcgtggtgcc ttacacccgt ggtgaagagg
     4981 taagccgtcc gtccgacgat attctgtttg agattgccca gcttgcggct cagggcgtgc
     5041 gtgaagtcaa cctgctcggt cagaacgtga acgcctggcg tggtgaaaac tacgacggca
     5101 ccaccggatc gtttgccgat ctgttgcgtc tggttgctgc gatcgacggg atcgatcgta
     5161 ttcgctttac caccagccat ccgatcgaat tcaccgacga tatcatcgaa gtgtatcgcg
     5221 acacgccgga gctggtgagc ttcctgcatc tgccggtaca gagcggttcc gatcgcattc
     5281 tgaacctgat gggacgtacc catacggcgc tggagtacaa agcgatcatc cgtaaactgc
     5341 gtgcggcgcg tccagatatt cagatcagtt ctgatttcat cgttggcttc cctggcgaaa
     5401 ccaccgaaga cttcgagaaa acgatgaagc tgattgccga cgtcaatttc gacatgagct
     5461 acagctttat cttctctgca cgtccgggta caccagccgc cgatatggtt gatgatgtcc
     5521 cggaagaaga gaagaagcag cgtctgtata ttctgcaaga gcgcattaat cagcaagcga
     5581 tggcgtggag ccgccggatg ctcggcacca cccagcgtat tctggtagaa ggtacatcgc
     5641 gtaagagcat catggagctt tccgggcgta cggaaaataa ccgcgtggtc aacttcgaag
     5701 gcacgccgga tatgatcggt aaattcgtcg atgtagaaat taccgacgtc tacccgaact
     5761 ctctgcgcgg taaagtggtg cgaactgaag atgaaatggg tctgcgcgtg gcagaaacac
     5821 cggaatcggt gatagcccgt actcgcaaag aaaacgacct tggcgtgggt tattatcagc
     5881 cgtaattctc aggcccgccg ttccggtggg ccttgttttt tctctcttta tccccatctt
     5941 tatcgcaatg cttgcgcctt tttcctgtgg cgtgaataat ttccttatag gccggtgaat
     6001 gttcagcttc cggcggcata cagataagag gaacggtttg aacatagaca ctcgcgaaat
     6061 caccctggag ccagcagaca acgcgcgtct gttgagcctg tgcggcccgt ttgatgacaa
     6121 catcaagcag ctcgaacgcc gtctcggcat cgagatcaat cgccgcgata accactttaa
     6181 actgaccggc cgtccgattt gcgtcaccgc tgcggcagac attctgcgta gcctgtatgt
     6241 cgatactgcc ccgatgcgcg gtcagattca ggatattgaa ccggaacaga tccaccttgc
     6301 gattaaagaa gcgcgggtat tggagcaaag tgcggagagc gtgccggagt acggcaaagc
     6361 ggtcaatatc aaaaccaaac gcggcgtaat taagccgcgc acgccaaacc aggcgcagta
     6421 catcgccaat attctcgacc atgacattac cttcggcgtt ggcccggcgg gtacgggtaa
     6481 aacctacctg gcagtggctg cggcagttga tgccctggag cgtcaggaaa ttcgccgtat
     6541 tctgctgact cgtccggcgg tcgaagccgg tgagaaactg ggcttcctgc ctggcgattt
     6601 aagccagaaa gtagacccgt atctgcgccc actgtacgac gcgctgtttg aaatgctggg
     6661 ctttgagaaa gtcgagaaac tgattgagcg caacgttatt gaagtcgcgc cgctggccta
     6721 tatgcgtggt cgtacgctga atgacgcctt tatcattctc gatgagagcc agaacaccac
     6781 catcgaacag atgaagatgt tcctgacccg tatcggtttt aactcaaaag cggttatcac
     6841 cggcgacgtc acacagatcg acctgccgcg taatactaaa tcaggcttac gtcatgccat
     6901 cgaagtgctg gccgatgtcg aagagatcag ctttaacttc ttccacagcg aagacgtggt
     6961 tcgtcacccg gtggtggcgc gtatcgttaa cgcctatgaa gcctgggaag aagccgaaca
     7021 aaaacgtaaa gcggcgctgg cggcagaacg caagcgcgaa gaacaggaac aaaaatgagt
     7081 caggtgatcc tcgatttaca actggcatgt gaagataact ccggattgcc ggaagagagc
     7141 cagtttcaaa catggctgaa tgcggtgatc ccgcagtttc aggaagagtc ggaagtgacg
     7201 attcgcgtgg tcgataccgc cgaaagccac agtctcaatc tgacctatcg cggtaaggat
     7261 aagccgacca acgtgctctc cttcccgttt gaagtgccgc ctggcatgga aatgtcgcta
     7321 ctgggcgatc tggttatctg ccgtcaggtg gttgagaagg aagctcagga gcaaggcaaa
     7381 ccactggagg cgcactgggc gcatatggtg gtgcacggca gtctgcattt gttaggttac
     7441 gatcacatcg aagatgacga agcagaagaa atggaagccc tcgaaacaga gattatgctt
     7501 gctctgggct atgaggatcc gtacattgcc gagaaagaat aatttgccag ccatttgact
     7561 ggcagacgtc cccgccgttg gtgattaacg acggcgtttt gattaactaa catgagaacc
     7621 catacgacgc catgagcgac gacaattcac acagtagtga cacgataagc aacaagaagg
     7681 gatttttctc cctgttactc agccaacttt tccacggtga accgaaaaac cgtgacgaac
     7741 tgctggcgct gatccgtgat tccgggcaga acgaccttat cgacgaagat acgcgcgata
     7801 tgctcgaagg ggtgatggac atcgcagacc aacgcgtccg cgacatcatg atcccccgct
     7861 cccagatgat taccctgaaa cgcaaccaga cgctggacga atgccttgat gtcatcatcg
     7921 agtccgccca ctcacgtttc ccggtgatta gcgaagacaa agatcacatt gaagggattc
     7981 tgatggcgaa agacttgctg ccgtttatgc gcagcgatgc tgaagccttc agcatggaca
     8041 aagtgttacg tcaggcagtt gtcgttcctg aaagtaagcg cgtagaccgg atgctgaaag
     8101 agtttcgctc tcagcgttac cacatggcga tcgttattga cgaattcggt ggggtttccg
     8161 gtctggtaac cattgaagac atcctggaac tgattgttgg tgagattgaa gacgaatatg
     8221 acgaagaaga tgatatcgac ttccgtcagc tgagtcgtca tacctggacc gtgcgcgcac
     8281 tggcttccat tgaagacttc aacgaagcgt tcggcaccca ctttagcgat gaagaagtcg
     8341 acactatcgg tggtctggtg atgcaggcat ttgggcatct tccggcacgt ggcgaaacca
     8401 tcgacatcga cggttaccag ttcaaagtgg cgatggccga cagtcggcgt attattcagg
     8461 ttcatgtcaa aatcccggat gactcacccc agccgaagct ggatgaataa aaccgaaact
     8521 ggatagataa ctacatggct tttgcctcat taattgaacg ccagcgcatt cgcctgctgc
     8581 tggcgttatt attcggtgcc tgcggaacgc tggccttctc tccttacgac gtctggcctg
     8641 cggcgattat ttcgctgatg gggcttcagg cgctgacctt taaccgccgt ccactccagt
     8701 ctgccgctat tggcttttgc tggggatttg gcctgtttgg cagcggtatt aactgggtct
     8761 atgtcagtat cgcgaccttt ggcggaatgc ctggcccggt taacatcttc ctggtggtgc
     8821 tgctggcggc gtatttgtcg ctgtataccg gactgtttgc tggcgtgttg tcgcgtctgt
     8881 ggtcgaaaac cacctggctg cgcgtggcga ttgtcgctcc tgctctctgg caagtgaccg
     8941 agtttctgcg cggttgggtg ctgactggct tcccgtggtt acagttcggc tatagccaga
     9001 ttgatggccc gttaaaaggg ctggcaccgc taatgggcgt ggaagccatt aacttcctgc
     9061 ttatgatggt tagcggcctg ctggcactgg cgttagtcaa acgcaactgg cgtccgctgg
     9121 tggtggccgt cgtgctgttt gcccttccct tcccgctgcg ttacatccag tggtttaccc
     9181 cacaaccgga gaaaaccatt caggtttcga tggttcaggg cgatattccg caatcgctga
     9241 aatgggacga aggccagctt cttaatacgc tgaagattta ctacaacgca acggcaccgc
     9301 tgatgggcaa atcatcgttg attatctggc cggagtcggc gataaccgat ctggaaatta
     9361 atcagcaacc gttcctcaaa gcactggacg gtgagttgcg tgataaaggg agtacgctgg
     9421 tgaccgggat tgttgacgcg cgtcttaata agcagaaccg ttacgatacc tacaacacca
     9481 tcatcacact gggtaaaggt gcaccgtaca gctacgaatc agccgatcgc tataacaaaa
     9541 accatctggt gccgtttggc gagtttgtcc cgctggagtc gattctgcgt ccgttagcac
     9601 cgttctttga tctgccgatg tcgtcgttca gccgtgggcc atatatccag ccgccgctgt
     9661 cggcaaatgg tattgagctt actgcggcta tttgctacga gatcattctc ggcgagcaag
     9721 tgcgcgataa cttccgcccg gataccgact atctgctgac tatctccaac gatgcgtggt
     9781 ttggtaagtc tattggtcca tggcagcact tccagatggc gcgaatgcgt gcgctggagc
     9841 tggcgcgccc attgctgcgt agtaccaaca acggcattac ggcggtgatt ggtccgcagg
     9901 gtgagattca ggcgatgatc cctcagttca cccgcgaagt gttgaccact aacgtgacgc
     9961 cgaccaccgg actcacacca tacgcacgta ccggcaactg gccgctgtgg gtgctgacgg
    10021 cactgtttgg ttttgccgct gtgctgatga gtctgcgtca acgacgtaaa taatcccttc
    10081 ttatcgtgcc ggaatacatc ccgtatttcg gcacgctcac cggattcggc atcagtgcgt
    10141 tatccggata acaaaattcc cctatccaac tttggcacat ctattgcttt gttatacaag
    10201 gcaaagcttg aaccagcatc agtacaaccc ggttgcacca cagcaatcca ccggtagcac
    10261 caaaagagtg cattttaaga attttgcttc ggaatggtgc ggcgcacctc ccaaatataa
    10321 acaataaaac agcaatatct ttacatttag ccaaactaaa tgttacctaa caggcacaac
    10381 actgcacaat aaagttgcag acgataacaa cacaaacact cacaacgggt atccatgcgt
    10441 tcttaacgca gaagataaag gagttggata tgcaattacg taaacctgcc acagcaatcc
    10501 tcgccctggc gctttccgca ggactggcac aggcagatga cgccgccccg gcagcaggca
    10561 gtacgctgga caaaatcgcc aaaaacggtg tgattgtcgt cggtcaccgt gaatcttcag
    10621 tacctttctc ttattacgac aatcagcaaa aagtggtggg ttactcgcag gattactcca
    10681 acgccattgt tgaagcagtg aaaaagaaac tcaacaaacc ggacttgcag gtgaaactga
    10741 ttccgatcac ctcacaaaac cgtattccac tgctgcaaaa cggcactttc gattttgaat
    10801 gtggttccac caccaataac gtcgaacgcc aaaaacaggc ggctttctct gacactattt
    10861 tcgtggtcgg tacgcgcctg ctgaccaaaa agggcggcga tatcaaagat tttgccgacc
    10921 tgaaaggcaa agccgtagtc gtcacttccg gcactacctc tgaagttttg ctcaacaaac
    10981 tgaatgaaga gcaaaaaatg aatatgcgca tcatcagcgc caaagatcac ggtgactctt
    11041 tccgcaccct ggaaagcggt cgtgccgttg cctttatgat ggatgatgcc ctgctggccg
    11101 gtgaacgtgc gaaagcgaag aaaccagaca actgggatat cgtcggcaag ccgcagtctc
    11161 aggaagccta cggttgcatg ttgcgtaaag acgatccgca gttcaaaaag ctgatggatg
    11221 acactatcgc tcaggtgcag acctccggtg aagcggaaaa atggtttgat aaatggttca
    11281 aaaatcccat tccgccgaaa aacctgaaca tgaatttcga actgtcagac gaaatgaaag
    11341 cactgttcaa agaaccgaat gacaaagcac tgaactaatt acaagaacca ggggcggaaa
    11401 ttctagccct ctcgattgtt acgtagcacg gacagactat acgcctgatg gtcgttcccc
    11461 atcgggcctg aaaaccgcaa tacgctgggt aacaatcttc gagggtagca gttaacgctg
    11521 ctaccctctt ttttctggag tagatttatg tctatagact ggaactgggg tattttttta
    11581 caacaagccc cgttcggcaa caccacctat cttggttgga tctggagtgg ttttcaggtg
    11641 acgatcgctt tgtcgatctg cgcctggatt atcgctttcc tcgtcggttc atttttcggc
    11701 attttacgta ccgttccaaa ccgttttctc tccggtctgg gtacgttgta tgtcgaactg
    11761 ttccgcaacg tgccgttgat tgtgcaattc tttacctggt atctggtgat cccggagttg
    11821 ctgccagaga aaatcggcat gtggtttaag gccgagctgg acccaaatat tcagtttttc
    11881 ctctcctcca tgctctgtct ggggctgttt accgccgccc gtgtttgcga acaggtacgc
    11941 gccgccattc agtcgctgcc gcgcgggcaa aagaatgccg cgctggcgat ggggctgacg
    12001 ctgccgcagg cttaccgcta tgtactgttg cctaatgctt atcgcgttat cgtcccgccg
    12061 atgacctccg agatgatgaa cctggtgaaa aactccgcca tcgcctcgac tatcggtctg
    12121 gtagatatgg cagcgcaggc gggtaaattg ctcgattact cggcccacgc ctgggagtcg
    12181 tttaccgcca ttacgctggc gtatgtgttg attaacgcgg ttatcatgct ggtaatgacg
    12241 ctggttgaac gtaaagttcg cctgcctggc aacatggggg gcaaataatg tacgagtttg
    12301 actggagttc cattgtccct tccctgccat atctgctcga cgggctggtg atcacgctga
    12361 aaatcaccgt cacggcagtc gtgattggta ttttgtgggg caccatgctg gcggtgatgc
    12421 gcttgtccag ctttgcgccc gtagcgtggt ttgccaaagc ctacgttaac gtattccgct
    12481 cgattccttt agttatggtt ttgctgtggt tttacctgat cgtgccgggt tttctgcaaa
    12541 acgtgctggg attatcgcca aaaaatgata tccgtttaat ttcggcgatg gtggcgtttt
    12601 cgatgtttga agcggcctac tattcagaga ttatccgtgc tggtattcaa agtatttctc
    12661 gtggtcagtc tagcgccgcg ctggcgttgg gaatgactca ctggcagtcg atgaaactga
    12721 ttattctgcc gcaggcgttc cgcgcgatgg tgccgctgct gctcactcag ggcatcgtac
    12781 tgttccagga tacctcgctg gtgtatgtgt tatctctggc cgatttcttc cgtaccgcct
    12841 caaccattgg tgagcgtgat ggtacgcagg ttgagatgat cctgtttgcc ggatttgttt
    12901 atttcgttat tagccttagc gcgtcgttgt tggtcagcta cttgaaaaga aggacagcat
    12961 aatgattacc ctgaaaaatg tttcaaaatg gtatggtcac tttcaggtgc tgaccgactg
    13021 ctcaaccgaa gtgaaaaaag gcgaagtggt ggtggtttgc ggcccgtctg gttccggcaa
    13081 atcaacgctg attaaaaccg tcaacggcct cgaaccggtg cagcaaggtg aaatcaccgt
    13141 cgatggtatc gtggttaacg ataagaaaac cgatctggca aagctgcgtt ctcgcgtcgg
    13201 gatggtattc caacatttcg agctgttccc tcatctgtcg attatcgaaa acctgaccct
    13261 ggcgcaggtg aaagtgctta aacgcgataa agcaccggct cgtgaaaaag cattgaaatt
    13321 gctggagcgc gtgggacttt ccgcacatgc caataaattt ccggcgcagc tttccggcgg
    13381 ccagcagcag cgtgtggcaa ttgctcgcgc attgtgtatg gatcctattg cgatgctgtt
    13441 cgacgaaccc acatcggcac tagacccgga gatgatcaac gaagtactgg acgtgatggt
    13501 ggaactggcg aacgagggga tgaccatgat ggtggtgacc cacgaaatgg gctttgcccg
    13561 taaagtggcg aatcgggtga tctttatgga tgagggtaaa attgttgaag actcgccgaa
    13621 agatgcgttc ttcgatgatc cgaaatcgga ccgcgcaaaa gacttcctcg cgaaaatcct
    13681 gcattaatca ctctggcgcg catacccaag tgcgcgccat ctcacgcttt atcgtctcgc
    13741 ccctcgtatc tgttacatcc tggcgttacc cttgtcgcaa agaagcacac aacaaggagc
    13801 aacaatggca ctgccaattc tgttagattg cgacccaggt catgatgacg ctatcgcaat
    13861 agttctcgcc ctcgcctcac cagagcttga tgtcaaagca attacgtctt ccgccggaaa
    13921 ccagacacca gaaaaaacct tacgcaatgt tctgcgtatg ctgaccttgc ttaatcgcac
    13981 cgatattccg gtggcaggcg gcgcggtaaa accgttaatg cgtgagttga ttatcgcgga
    14041 caatgtgcac ggcgaaagcg gtctcgacgg cccggcatta ccggaaccga cgtttgcacc
    14101 gcaaaagtgt acggcggtgg agctgatggc gaaaacgcta cgtgaaagtg cggaacctgt
    14161 caccattgtt tctaccggac cgcaaactaa cgttgctttg ctgctcaata gccatccgga
    14221 actgcatagc aaaattgccc gtatcgtgat tatgggcggc gcaatggggc tgggtaactg
    14281 gacgcctgcg gctgaattta atatttacgt tgacccagaa gcggcagaaa ttgtattcca
    14341 gtcagggatc ccggtggtga tggccggtct ggatgttact cacaaagcac aaattcacgg
    14401 tgaggacacc gagcgtttcc gcgcgattgg caaccctgtt tcaaccattg ttgccgaact
    14461 gctggatttc ttcctcgaat atcataaaga cgaaaaatgg ggctttgtcg gcgcaccgct
    14521 gcatgaccca tgcaccatcg cctggctgtt gaaacccgag ttgtttacca ctgttgagcg
    14581 ctgggttggc gtggaaacac aggggaaata tacccagggg atgacggttg ttgattatta
    14641 ttatctgact ggcaataaac cgaatgccac tgtaatggtc gatgttgatc gtcagggctt
    14701 tgttgattta ctggctgatc gtctgaaatt ttacgcttaa cacacgtcat tgattaagat
    14761 caccacgcgt tacctgggtg gtcttaatct gtttatctct ttttctttca agatataaat
    14821 gcaatggata atgcagaact cgccattggt atcgatctcg gtactaccaa tagtttaatt
    14881 gccgtctgga aagacggtgc cgcgcaatta attccaaata agttcggtga atatttaaca
    14941 ccatccataa ttagcatgga tgaaaataat catattttag tcggaaaacc ggctgtatca
    15001 cggcgtactt cgcatccgga taaaacggca gcgttattta aacgtgcaat gggcagtaat
    15061 accaactggc ggttaggcag cgacacattt aacgcgccag aactgtcctc tttggtatta
    15121 cgctcattaa aagaagatgc cgaagaattt ctgcaacgtc cgattaaaga tgtggtgatc
    15181 tctgttccgg cttatttcag cgatgaacaa cgcaagcata cccgtttagc agcggagtta
    15241 gccggtttaa atgcggtacg cttaattaat gaacccacag cagctgcgat ggcgtatggc
    15301 ctgcataccc aacaaaatac ccgttcgctg gtgtttgatc tcggtggcgg cacgtttgac
    15361 gttacggtgc ttgagtacgc cacgccggtg attgaagttc acacctccgc tggcgacaac
    15421 ttcctcggtg gcgaagattt tacccatatg ctggtcgatg aggttttaaa acgcgcggat
    15481 gtcgccagga ccacgcttaa cgagagtgaa ctggcagcct tgtatgcctg tgtggaagcg
    15541 gcaaaatgta gcaatcaatc gccaatgcac attcgctggc agtaccagga tgaaacgcgg
    15601 gaatgcgaat tttacgagaa cgaactggaa gatttgtggt tgccgctgct caatcgcttg
    15661 cgagtgccga ttgaacaggc gttgcgcgat gcacgtctga agccgagtca aatcgacagt
    15721 ctggtgctgg ttggcggcgc gtcacaaatg ccgctggtgc agcgaatcgc cgtgcgtctg
    15781 tttggcaaat taccgtatca aagttacgat ccgagcacca ttgtcgcgct gggcgcagca
    15841 attcaggctg cctgccgctt acgcagtgaa gatattgaag aagtgatcct cactgatatt
    15901 tgcccttact cgttgggcgt tgaggttaac cgtcagggcg tttccggcat tttctcgccg
    15961 attattgaac gaaacaccac tgtgcccgtg tcgcgtgtag aaacttattc aaccatgcac
    16021 ccggaacagg attcaattac ggttaacgtc tatcagggag aaaaccacaa agttaaaaac
    16081 aacattctgg tggaatcctt cgatgtgccg ttgaagaaaa ccggggctta tcagtcgatt
    16141 gatattcgct ttagttatga tatcaacggt ttgcttgaag ttgacgtgct tctggaagac
    16201 ggcagcgtta agtccagagt gattaaccac agcccggtaa cattgagcgc gcagcagatt
    16261 gaagagagtc ggacgcggtt atccgcattg aaaatttatc cgcgcgatat gctcatcaat
    16321 cgtaccttta aagccaaact ggaagagttg tgggcgcggg cgctgggtga cgagcgagaa
    16381 gagattggcc gggtgatcac cgattttgat gcggcgctgc agtcaaacga tatggcccgc
    16441 gtcgatgagg tcaggcggcg ggcgagcgtt tatttagcca ttgagatccc ataatcacgg
    16501 ctgccggatg cggcgtaaat gctgcatccg gcatcggcgc aaaaaacaca tcgtcaacta
    16561 aacaacatcg cgattttgcc gagagcataa cctataacca gcaccgttaa actgattttg
    16621 ataatgctgg taatgctctg tggtttttca ccctcatcat ttttttcttc atggttttca
    16681 ggtgttggct catcgacatc ttcatcctcc ggctcgagtt ttactttcgt caacagcatc
    16741 cgggtgataa tggcctctaa tggatgagca tctccgcctt ccagccttgt attaagacgg
    16801 gccagcatct cttgtttcgg aaacgcttca agtactacct gttccgctac ccgatacaaa
    16861 tccagtaacg gtgaatcttg ctctgtctcg ctaccgactc tcgatttcca ccacgcccat
    16921 gctttgcgtt tgaagaataa cgactcaata aacgattgac ctaacggcga tagttttccg
    16981 ctatgtaaaa tttccgccca taacagacgc gtccgggcgc tctcaccagg aacatatttc
    17041 aacggtttac cttgcgcatc taccatgctc cgcgcttcga taaccatcac taataacgcg
    17101 aaataatccg ggcaatgttg tgcgctccac tgcaacaaca aattatcggc ttgtgtagat
    17161 gggtaacttc cccagtacgc gcagagatcg gcaagcaggc tttccccctc gccgcagtac
    17221 aaacgttgtg cacactggta ataccgggca tcttcgcttt ctggttcttc catttgccac
    17281 tgtttggcta caggaatgag ttccgccata cctgtttgca ccgagctgta ccagcgcaac
    17341 agtttgcgat gtaactttgc atcatcagga atgaaccacg gaccttccat cgccaaaaaa
    17401 gcagtgacat agtcaggatg gtaatggcag caacgttcca gcgcgaagta atattcaaca
    17461 gtctggttct gcaacgcaac gggatattcc agaaggccaa ggaaatcgaa acaatcacca
    17521 gcctgaatgg cctcaagaaa agcctccctt tcttcctcat ccattcctac gctgtcatta
    17581 aacgaatgcc agttcaaacg ctctgccagc aaagaagcac aatttaatga aagatatcgc
    17641 gcttctatag cgatggcaca cagcggccag cgtaattgat cgacatcctc catgttccag
    17701 gtatttaatt gctgaataaa tttctgccag gcggagggct gaaaacgatc actttctgaa
    17761 tccagtaatg ccctgaatac acgtagtatt tcatgctcag tggcatcatc tttttcttca
    17821 tcatctgctt cctcgacagg atttaccgcc agtcgcagcg cctcttcata ggcctggcgt
    17881 aatgctttaa acccctgcgg atcggtttcg ggatgacata acggtaagcg agcaagataa
    17941 gcctggcgga taatgtctat ttgcgtcgtg ctttcaattt cgaggatttg ccaacatgtt
    18001 ttcataggtc gctcacatcc ttaagcgtta ccgggcattc aggctggaca gttaatggct
    18061 gccagggaag cgtggcatag aaacgatcgc ttcccgctgc cagttcttcg aacatacgac
    18121 tcccggcgct atcgtatttg tacagggtta gcgcctcgcg tagcgaagtg ttgtcttcgc
    18181 actgagaaga ttgccagtag agacgcccta gtgaataggc agaaaaatag tgcatccagc
    18241 tgtgatagta atggtgagct cgtacataga cgcgcgattg cagccataaa ctctcgtctt
    18301 ccgtcagcca cttgttgagc acaccgaccc gtgacagaaa ccccattcga acataatccc
    18361 aagctttgat tcccccttct ccacagcaca tagcggtttg ggcaacgaat tgggtataaa
    18421 tcaaccctct ctccgagcca ccagcaatta atgctttcca ctcttcaggg gaagagcgat
    18481 gccatgtcag atataagcca gctaaatcat tagcatgacc atcatcagtc atctgataaa
    18541 tagtctgaat taaatcatca cgattattta ttccccagga tgatgagaga tcgaattctt
    18601 ttatcccggt atacattttt ggcgagtcat gcgcgggttt gcaaccctga ttaagtattt
    18661 ccatgggcgc agaaagcgca aaaagaagat attgttcttc cttattcatt tgttaataat
    18721 cccttaagt
//
LOCUS       DAZKFU020000080        18303 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZKFU020000080 DAZKFU020000000
VERSION     DAZKFU020000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.080"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(114..761)
                     /gene="ychE"
                     /locus_tag="ACHOQQ_RS10940"
                     /old_locus_tag="pgaptmp_002188"
     CDS             complement(114..761)
                     /gene="ychE"
                     /locus_tag="ACHOQQ_RS10940"
                     /old_locus_tag="pgaptmp_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGU4470434.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSFQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(792..845)
                     /gene="ychE"
                     /locus_tag="ACHOQQ_RS10945"
                     /old_locus_tag="pgaptmp_002189"
     CDS             complement(792..845)
                     /gene="ychE"
                     /locus_tag="ACHOQQ_RS10945"
                     /old_locus_tag="pgaptmp_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGU4470435.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1238..3913
                     /gene="adhE"
                     /locus_tag="ACHOQQ_RS10950"
                     /old_locus_tag="pgaptmp_002190"
                     /gene_synonym="adhC"
     CDS             1238..3913
                     /gene="adhE"
                     /locus_tag="ACHOQQ_RS10950"
                     /old_locus_tag="pgaptmp_002190"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGU4470436.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4215..4832)
                     /gene="tdk"
                     /locus_tag="ACHOQQ_RS10955"
                     /old_locus_tag="pgaptmp_002191"
     CDS             complement(4215..4832)
                     /gene="tdk"
                     /locus_tag="ACHOQQ_RS10955"
                     /old_locus_tag="pgaptmp_002191"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGU4470437.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5436..5849
                     /gene="hns"
                     /locus_tag="ACHOQQ_RS10960"
                     /old_locus_tag="pgaptmp_002192"
     CDS             5436..5849
                     /gene="hns"
                     /locus_tag="ACHOQQ_RS10960"
                     /old_locus_tag="pgaptmp_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGU4470438.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(5993..6901)
                     /gene="galU"
                     /locus_tag="ACHOQQ_RS10965"
                     /old_locus_tag="pgaptmp_002193"
     CDS             complement(5993..6901)
                     /gene="galU"
                     /locus_tag="ACHOQQ_RS10965"
                     /old_locus_tag="pgaptmp_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGU4470439.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7103..8116)
                     /gene="rssB"
                     /locus_tag="ACHOQQ_RS10970"
                     /old_locus_tag="pgaptmp_002194"
     CDS             complement(7103..8116)
                     /gene="rssB"
                     /locus_tag="ACHOQQ_RS10970"
                     /old_locus_tag="pgaptmp_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGU4470440.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8208..9113)
                     /gene="rssA"
                     /locus_tag="ACHOQQ_RS10975"
                     /old_locus_tag="pgaptmp_002195"
     CDS             complement(8208..9113)
                     /gene="rssA"
                     /locus_tag="ACHOQQ_RS10975"
                     /old_locus_tag="pgaptmp_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGU4470441.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9226..9684
                     /gene="ychJ"
                     /locus_tag="ACHOQQ_RS10980"
                     /old_locus_tag="pgaptmp_002196"
     CDS             9226..9684
                     /gene="ychJ"
                     /locus_tag="ACHOQQ_RS10980"
                     /old_locus_tag="pgaptmp_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGU4470442.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAVLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9734..10576
                     /gene="purU"
                     /locus_tag="ACHOQQ_RS10985"
                     /old_locus_tag="pgaptmp_002197"
     CDS             9734..10576
                     /gene="purU"
                     /locus_tag="ACHOQQ_RS10985"
                     /old_locus_tag="pgaptmp_002197"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGU4470443.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10736..10820
                     /locus_tag="ACHOQQ_RS10990"
                     /old_locus_tag="pgaptmp_002198"
     tRNA            10736..10820
                     /locus_tag="ACHOQQ_RS10990"
                     /old_locus_tag="pgaptmp_002198"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10770..10772,aa:Tyr,seq:gta)
     gene            10864..10995
                     /locus_tag="ACHOQQ_RS10995"
                     /old_locus_tag="pgaptmp_002199"
     ncRNA           10864..10995
                     /ncRNA_class="other"
                     /locus_tag="ACHOQQ_RS10995"
                     /old_locus_tag="pgaptmp_002199"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            11030..11114
                     /locus_tag="ACHOQQ_RS11000"
                     /old_locus_tag="pgaptmp_002200"
     tRNA            11030..11114
                     /locus_tag="ACHOQQ_RS11000"
                     /old_locus_tag="pgaptmp_002200"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11064..11066,aa:Tyr,seq:gta)
     gene            11161..11292
                     /locus_tag="ACHOQQ_RS11005"
                     /old_locus_tag="pgaptmp_002201"
     ncRNA           11161..11292
                     /ncRNA_class="other"
                     /locus_tag="ACHOQQ_RS11005"
                     /old_locus_tag="pgaptmp_002201"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11476..12153)
                     /gene="narI"
                     /locus_tag="ACHOQQ_RS11010"
                     /old_locus_tag="pgaptmp_002202"
     CDS             complement(11476..12153)
                     /gene="narI"
                     /locus_tag="ACHOQQ_RS11010"
                     /old_locus_tag="pgaptmp_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4470444.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12153..12863)
                     /gene="narJ"
                     /locus_tag="ACHOQQ_RS11015"
                     /old_locus_tag="pgaptmp_002203"
     CDS             complement(12153..12863)
                     /gene="narJ"
                     /locus_tag="ACHOQQ_RS11015"
                     /old_locus_tag="pgaptmp_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4470445.1"
                     /translation="MIELVIVSRLLEYPDAALWQYQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LMKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(12860..14398)
                     /gene="narH"
                     /locus_tag="ACHOQQ_RS11020"
                     /old_locus_tag="pgaptmp_002204"
     CDS             complement(12860..14398)
                     /gene="narH"
                     /locus_tag="ACHOQQ_RS11020"
                     /old_locus_tag="pgaptmp_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4470446.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMV
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14395..18138)
                     /gene="narG"
                     /locus_tag="ACHOQQ_RS11025"
                     /old_locus_tag="pgaptmp_002205"
     CDS             complement(14395..18138)
                     /gene="narG"
                     /locus_tag="ACHOQQ_RS11025"
                     /old_locus_tag="pgaptmp_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGU4470447.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4615 a         4793 c         4380 g         4515 t
ORIGIN      
        1 tttgagaaga atatttttac ctattccttt tgcttttgat aactgcgcgg gaacaaagta
       61 aaaataatga cgaagtaaac ggagcatggc agctccgttt catttgaaag gaattaatta
      121 agcaggccgg ggaaaatccc cttaatacca gtaacgataa attcaatccc caatgccatc
      181 agcaatagcc ccataatacg cgtaatcacg ttgatgccgg tctggcgtaa aacccgtacc
      241 agccacggtg ccatgcggaa caatccccaa caacataaag cgaacaatgc aatagccaca
      301 aagaaaccaa acagatagct aatgctgtga taacgcgtac cccagacgat ggtagaactg
      361 atcgcccctg gccccgccat caacggcaac gccagtggca ccacaccaat gctttcacgt
      421 accgcggttt ctgatttttc ttgcttgttc tgtttatcct cgccaagctt gccgctgatc
      481 atcgacatcg ctattgtcac caccaggata cccccggcga tacggaacga atcaattgat
      541 ataccaaaaa gttgtagaat cgtgtcgccg agaaaaagcg agatccacaa gataatggcc
      601 acagacaggt tggctgtaag gttagttttg tttcgcgctg ccgctgtctg aaaactggtc
      661 atgctgataa agacgggaat aatccctacc gggttgacca gcgcaaataa cccgatgaaa
      721 aatttgaagt aaacgggaaa atcaaaaagg gtctgaatca cggttagctc cgaagcaaaa
      781 gccggataat gttagccata aataaggttg aaaagacgcg ctgacaatac gccttttgac
      841 agcatttttc acctcctaac tacttaaaat tgctatcatt cgttattgtt atctagttgt
      901 gcaaaacatg ctaatgtagc caccaaatca tactacaatt tattaactgt tagctataat
      961 ggcgaaaagc gatgctgaaa ggtgtcagct ttgcaaaaat ttgatttgga tcacgtaatc
     1021 agtacccaga agtgagtaat cttgcttacg ccacctggaa gtgacgcatt agagataata
     1081 actctgatgt ttaaactctt ttagtaaatc acagtgagtg tgagcgcgag taagcttttg
     1141 attttcatcg gttaagcaaa tcatcaccgc actgactata ctctcgtatt cgagcagatg
     1201 atttactaaa aaagtttaac attatcagga gagcattatg gctgttacta atgtcgctga
     1261 acttaacgca ctcgtagagc gtgtaaaaaa agcccagcgt gaatatgcca gtttcactca
     1321 agagcaagta gacaaaatct tccgcgccgc cgctctggct gctgcagatg ctcgaatccc
     1381 actcgcgaaa atggccgttg ccgaatccgg catgggtatc gtcgaagata aagtgatcaa
     1441 aaaccacttt gcttctgaat atatctacaa cgcctataaa gatgaaaaaa cctgtggtgt
     1501 tctgtctgaa gacgacactt ttggtaccat cactatcgct gaaccaatcg gtattatttg
     1561 cggtatcgtt ccgaccacta acccgacttc aactgctatc ttcaaatcgc tgatcagtct
     1621 gaagacccgt aacgccatta tcttctcccc gcacccgcgt gcaaaagatg ccaccaacaa
     1681 agcggctgat atcgttctgc aggctgctat cgctgccggt gctccgaaag atctgatcgg
     1741 ctggatcgat caaccttctg ttgaactgtc taacgcactg atgcaccacc cagacatcaa
     1801 cctgatcctc gcgactggtg gtccgggcat ggttaaagcc gcatacagct ccggtaaacc
     1861 agctatcggc gtaggcgcgg gtaacactcc agttgttatt gatgaaactg ctgatatcaa
     1921 acgtgcagtt gcatctgtac tgatgtccaa aaccttcgac aacggcgtaa tctgtgcttc
     1981 tgaacagtct gttgttgttg ttgactctgt ttatgacgca gtacgtgaac gtttcgcaac
     2041 ccacggcggc tatctgttgc agggtaaaga gctgaaagct gttcaggacg ttatcctgaa
     2101 aaacggtgcg ctgaacgcgg ctatcgttgg tcagccagcc tataaaattg ctgaactggc
     2161 aggcttctct gtaccagaaa acaccaagat tctgatcggt gaagtgaccg ttgttgatga
     2221 aagcgaaccg ttcgcacatg aaaaactgtc cccgactctg gcaatgtacc gcgctaaaga
     2281 tttcgaagac gcggtagaaa aagcagagaa actggttgct atgggtggta tcggtcatac
     2341 ctcttgcctg tacactgacc aggataacca accggctcgc gtttcttact tcggtcagaa
     2401 aatgaaaacg gctcgtatcc tgattaacac cccggcttct cagggtggta tcggtgacct
     2461 gtataacttc aaactcgcac cttccctgac tctgggttgt ggttcctggg gtggtaactc
     2521 catctctgaa aacgttggtc cgaaacacct gatcaacaag aaaaccgttg ctaagcgagc
     2581 tgaaaacatg ttgtggcaca aacttccgaa atctatctac ttccgccgtg gctccctgcc
     2641 aatcgcgctg gatgaagtga ttactgatgg ccacaaacgt gcgctcatcg tgactgaccg
     2701 cttcctgttt aacaatggtt atgctgatca gatcacttcc gtactgaaag cagcaggcgt
     2761 tgaaactgaa gtcttcttcg aagtagaagc tgacccgacc ctgagcatcg ttcgtaaagg
     2821 tgcagaactg gcaaactcct tcaaaccaga cgtgattatc gcgctgggtg gtggttcccc
     2881 gatggacgcc gcgaagatca tgtgggttat gtacgaacat ccggaaactc acttcgaaga
     2941 gctggcgctg cgctttatgg atatccgtaa acgtatctac aagttcccga aaatgggcgt
     3001 gaaagcgaaa atgatcgctg tcaccaccac ttctggtaca ggttctgaag tcactccgtt
     3061 tgcggttgta actgacgacg ctactggtca gaaatatccg ctggcagact atgcgctgac
     3121 cccggatatg gcgattgtcg acgccaacct ggttatggac atgccgaagt ccctgtgtgc
     3181 tttcggtggt ctggacgcag taactcacgc catggaagct tatgtttctg tactggcatc
     3241 tgagttctct gatggtcagg ctctgcaggc actgaaactg ctgaaagaat atctgccagc
     3301 gtcctaccac gaagggtcta aaaatccggt agcgcgtgaa cgtgttcaca gtgcagcgac
     3361 tatcgcgggt atcgcgtttg cgaacgcctt cctgggtgta tgtcactcaa tggcgcacaa
     3421 actgggttcc cagttccata ttccgcacgg tctggcaaac gccctgctga tttgtaacgt
     3481 tattcgctac aatgcgaacg acaacccgac caagcagact gcattcagcc agtatgaccg
     3541 tccgcaggct cgccgtcgtt atgctgaaat tgccgaccac ctgggtctga gcgcaccggg
     3601 cgaccgtact gctgctaaga tcgagaaact gctggcatgg ctggaaacgc tgaaggctga
     3661 actgggtatt ccgaaatcta tccgtgaagc tggcgttcag gaagcagact tcctggcgaa
     3721 cgtggataaa ctgtctgaag atgcattcga tgaccagtgc accggcgcta acccgcgtta
     3781 cccgctgatc tccgagctga aacagattct gctggatacc tactacggtc gtgattatgt
     3841 agaaggtgaa actgcagcga aaaaagaagc cgctccggct aaagctgaga aaaaagcgaa
     3901 aaaatccgct taatcagtag cgctgtctgg caacataaac ggccccttct ggggccgttt
     3961 ttttgtttac ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc
     4021 gacgatgtat aacgcctaaa cacagcgata ttgtacttta cagatcacaa gtcaacgtcg
     4081 gtgcttaaga gccctgtgag gcgtatagcg gcgttaaaaa actgccgaga agggtgtata
     4141 gcccggaaga agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag
     4201 taaagaaatt cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact
     4261 tgtaacgcct ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaata
     4321 accacctgct caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt
     4381 gccttacggc cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt
     4441 aagtattggc tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt
     4501 acgggtatat cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa
     4561 aactggcatt catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc
     4621 tcatcaaata atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc
     4681 gaactgactt tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc
     4741 atgccgcgtt cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc
     4801 attgcggaat agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat
     4861 aaagttggct ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag
     4921 tggtatatgc gttctccctt acgaagcctt gcataatcct tctgagctat cattacaact
     4981 gcctcgcttg ttataagcgg gtaaatgact gctggtaact attcacaatc tttaacctgt
     5041 tgcgcaagta atagccctct gttgacctcc aggagatagt gcaatactaa gtccctgctc
     5101 ttattgcgac tgttctactt ttcatcattc gcttaatagg gaattctcgt aaacacgact
     5161 aatacagaag actgaaaggt cgtcagctta cgataatctc cccataaaat gtgacatgaa
     5221 tcaggaagtt ttaacctcac gtgctgcgaa atcatcggta caaatagggc tatatgccgc
     5281 gtcttttctg gctaatttta tgaaaagata tttattggcg gcacaaaata aagaacaatt
     5341 ttgaattact tacattcctg gctattgcac aactgaattt aaggctctat tattacctca
     5401 acaaaccacc ccaatataag tttgagatta ctacaatgag cgaagcactt aaaattctga
     5461 acaacatccg tactcttcgt gcgcaggcaa gagaatgtac acttgaaacg ctggaagaaa
     5521 tgctggaaaa attagaagtt gttgttaacg aacgtcgcga agaagaaagc gcggctgctg
     5581 ctgaagttga agagcgcact cgtaaactgc agcaatatcg cgaaatgctg atcgctgacg
     5641 gtattgaccc gaacgagctg ctgaatagcc ttgccgccgt taaatctggc accaaagcta
     5701 aacgtgctca gcgtccggca aaatatagct acgttgacga aaacggcgaa actaaaacct
     5761 ggactggcca gggccgtact ccagctgtaa tcaaaaaagc aatggatgag caaggtaaat
     5821 ccctcgacga tttcctgatc aagcaataat cttttgtaga ttgcacttgc ttaaaatccc
     5881 gccatcggcg ggatttttta ttgtccggtt taagacaatt taataagaat aagctataaa
     5941 aaaacggcgt cgattgctca acgccgtttc gtggataaca ccgatacgga tgttacttct
     6001 taatgcccat ctcttcttca agccaggctt taaattccgt gccaagggtg ttatgacgaa
     6061 taccgtattc aacgaaggcc tgcatgtaac ctaatttatt accgcagtca tggctcttcc
     6121 ctttcatatg ataggcttca accgtttctt tttcgattag catatcaatt gcgtcggtga
     6181 gctgaatttc atcaccagct cccggagggg tttttgccag caacggccaa atatccgcac
     6241 taagtacgta acgacccaca atagcgagat tagacggcgc aacatccgct tttggttttt
     6301 caaccacgcc aaccatcggt acgctttcac ccggcgctaa ttcaacgcct ttgcaatcca
     6361 caacgccata tgcggtcaca tcagcaaccg gttcaaccat gatctggcta tgacccgttt
     6421 catcgaagcg gcggatcatc tctgccaggt tatcctgtga caaatcggat tcatattcat
     6481 ccagaataac gtcaggcaaa ataacagcca caggttcatc acccactacc gggtgagcac
     6541 acaataccgc gtgtcccagg cctttcgcca gcccctgacg aacttgcata atagttacgt
     6601 gcggcggaca aatagactgc acttcatcaa gcagttgacg ttttacacgt ttttccagca
     6661 ttgcttccag ttcaaaactg gtatcaaagt ggttttcaat agagttttta gatgagtgtg
     6721 taaccagcac aatttcagta atgccagccg caatacattc attcacgacg tattgaatta
     6781 atggcttatc gacaagtggc agcatctctt tcgggatggc tttcgtcgcc ggcaacatcc
     6841 tggttcctaa tcccgcaacg gggataacgg cttttttgac tttcgtatta atggcagcca
     6901 tttaaattct cctggactgt tcgtgttttg aacgtgttca tatttctgta tcgcatccca
     6961 gtatatcagc accccggagg ggctaagttc tgaaaaggat gcgagttagc ataattaaga
     7021 caattactta tacgaatggc gacgatagta ccaccagccg acattagctg gtaatgcaaa
     7081 tttagcccgc gtcatcgttt gctcattctg cagacaacat caagcgcagt cgaccaccgg
     7141 ttccccatat ttggcattgc caggcatcgc atcgctggct caattgattc aaataagcgt
     7201 tacctaaagt gcctaacgga acaccattac tgatttgcac ctggtgttcg ccggtattta
     7261 acgtcgcatt cagacccgca gaaaccagaa tgagattttt cagttcgcga tgataatagc
     7321 caactaatag cggaaactgc cccggcagat tggcctgacg gagtaaatgg tttacctgct
     7381 tcaataacgc gcccaactct ggcaaccgtt gattttggtg tgcaagctgt tcctgtaata
     7441 atccgttaaa caatgcgcgt aataacaagg cagcaagtac gccattatgt ccagctcggg
     7501 ttacatcaag gcaataaaat gccagatcgt tttccgaaag tgcggcaata tcaagcacca
     7561 ggccgggttt gtccgcggca accaattgac ggtaattaac ccggcaatgg gaaatcacct
     7621 gctgaaccgg cggttgtagt tcctgtaata atttcgccgc tgcggcaggg ttatcaacca
     7681 ttgcatccca gtcgcgaaaa agcctttcct cttcctcaac gcgcgaatta aacatgctgg
     7741 gatagagaca ggcaaaaacc atctcgcgca agcgattcag atctttaact ggtttcagca
     7801 aaacatcttc aacgcccaga cgtaacgctt tggcaatatc tgccatattt tcagtggcag
     7861 atatcaccag aactggggtc tggtcgcctc tgttacgtat atgctccagc agtttaagcc
     7921 cgttcattcg tggcatcgcg atatcacata tcatcaggtc tggagtgaaa cctcccagca
     7981 actcaagggc atccacccca tcagccgcca gtaccgttgt cgctcccaat gaggaaaacc
     8041 acgaatccag aagcgagcga aatacctgct catcttcaac aatgagaatc tgttttccga
     8101 ccaatggctg cgtcatgttc tctcccctga ctggctttac tcaatagtgg catgctattg
     8161 ccacctgcgc ctgtcagaat taacttaagt gtagataaaa attctggtca aatgttggtg
     8221 cgtaccaacg gcaaaagttc gtccattttc ttttccactg ccagctgccc ggccgcaatg
     8281 gcagcgtgcg cgcgatggaa atcaagcgta gatatttgcg ggcaaacagg ttgaatcaga
     8341 atatcgggcg gatcacctgc catgcggttc cttttaaggc ggttctccag cacctggata
     8401 gaagtggtca taatctctgt tgccgttggt gctgtcaccg cccgacgtgt cgttatgctg
     8461 cctaaccttt ctttcagacg cgcatgccac ggcagagaat caccattctc gctattttct
     8521 tcactgacat taaaggagag caaatcttgt tgcatcaaat gagcatcgtg ctgcaggtcg
     8581 accgctatca caatatcagc acccaatgca cgcgtgagcg aaataggaat tgggttaacg
     8641 actgccccat caaccagcca gtagccgtta tgcgcaacgg gtgccattag tcctggaata
     8701 ctgcatgatg cgcgaatagc aagatggaga tcgccttcag taaaccataa ttcacgccca
     8761 gtacttaaat tggtggcaac agccgcgaag cgacgggaac aattttcgat ctctgtttcc
     8821 ggcattattt cgcggtattg attgaagaca cgctcgccgc gcagtaaccc accgcgctgc
     8881 caggagagat ccatcaggcg taaaacatcc caataactga aagaggttac ccaatcttcc
     8941 agcgcagata atcgatcgca tgcataggcc gcgcccacca gcgaaccaat agaacatcct
     9001 gcaacgatat caatttcaat acccactttt tttagcgcat taataacgcc aatatgcgac
     9061 caacctctcg ccgcgccaga tcccagcgcc agccctattt ttatctttct cattattccc
     9121 gcaagattcc cctggaatgc aatcgtagcc acatcgctac tgctcggtta acatagtgct
     9181 actcaggcgg ttaatacgcc gttattgttt ccagggagat tatttgtgtc tcagctttgt
     9241 ccctgtggta gtgctgtcga gtatagccta tgttgccacc cttatgtgtc tggtgaaaag
     9301 gttgcacctg atccagaaca tctcatgcgt tcgcgttact gcgcttttgt gatgcaagac
     9361 gcagattatt taataaagac ctggcatcca tcttgtgggg cagcggtgtt acgtgccgaa
     9421 ttgattgccg gatttgcgca tactgaatgg ttgggattga ccgtatttga acattgttgg
     9481 caggacgtcg acaatatcgg atttgtcagt tttgttgctc gatttaccga aggtggcaaa
     9541 acaggtgcaa taattgaacg ttcgcgattt ttgaaagaaa acggtcaatg gtactatatt
     9601 gacggtacac gtccgcagtt tggccgcaac gatccctgcc cttgtggttc aggtaaaaaa
     9661 tttaaaaagt gctgcggcca ataatggttg acggtacggt ttagcaaaca ctctcaacaa
     9721 ggtttttcca gcaatgcatt cactccaacg taaagttctg cgtactattt gtccggacca
     9781 aaaaggtctg atcgcacgta ttaccaatat ttgctacaag cacgagttaa atatcgtaca
     9841 gaacaatgaa tttgttgatc accgtaccgg gcgctttttt atgcgcacgg aactggaagg
     9901 gatttttaat gattccaccc tgctggcgga tctcgatagc gcattgccag aaggctccgt
     9961 gcgtgagctg aatcctgccg gtcgtcgccg gatagtgatt ctggtcacta aagaagcgca
    10021 ttgccttggc gatttgttga tgaaagccaa ttacggcggc ctggatgtcg aaatcgcggc
    10081 agttattggt aaccacgata ctttacgttc tctggttgag cgttttgata taccgtttga
    10141 gctggtaagc catgaagggt taagccgcaa cgagcacgat caaaagatgg cggatgccat
    10201 tgatgcttat caacctgact acgtggtgct ggcgaagtat atgcgggtat taacaccgga
    10261 atttgtgtca cgcttcccga ataagatcat caatattcac cattccttcc tgccagcgtt
    10321 tatcggcgca cgtccttatc accaggccta tgaacgtggc gtgaagatta ttggtgcaac
    10381 cgctcactat gtgaatgaca atctggacga aggcccaatc atcatgcagg acgttattca
    10441 tgtcgatcat acctacacag ctgaagatat gatgcgcgca ggtcgtgacg tcgagaaaaa
    10501 cgtcttaagt cgtgcgcttt acaaagtact ggcgcagcgc gtctttgttt acggtaatcg
    10561 gacgattatt ctttaatcgc cagcaaaaat aactggttac ctttaatccg ttacggataa
    10621 aaattacgca accagttcat ttttctcaac gtaacacttt acagcggcgc gtcatttgat
    10681 atgatgcgcc ccgcttcccg ataagggagc aggccagtaa aaagcattac cccgtggtgg
    10741 ggttcccgag cggccaaagg gagcagactg taaatctgcc gtcatcgact tcgaaggttc
    10801 gaatccttcc cccaccacca tgattcacca cagcgatgtg gtaacttcgg tgagattgtg
    10861 aaagtttgca ctcatcgggg aagggtgaga accttcgact aaggttcgac tcgagcgaaa
    10921 gcgagagaac attgccacag gcaacggccc gaagggtgag gagcgaagcg acgaataatc
    10981 cttcccccac cagcacctca agcattacct caaaatttga attacctctg gtggggttcc
    11041 cgagcggcca aagggagcag actgtaaatc tgccgtcatc gacttcgaag gttcgaatcc
    11101 ttcccccacc accatcactt tcaaaagtcc ctgaacttcc caacgaatcc gcaattaaat
    11161 attctgccca tgcggggaag gatgagaagc ttcgaccaag gttcgactcg agcgccaatg
    11221 agaaagcgtt gccgcaggca actacccgaa gggcgaagcg cgcagcgctg agtaatcctt
    11281 cccccaccac catttcttcc tgaaatactc aaaacctgtt actccgagct aattctgccc
    11341 gatggcaccg cgtttaccgg acctacaata tcctcccctg taggtcggat aagcgaagcg
    11401 catccgacaa ctcaccccgc accgtgccca taaaaaaacc ccgccgaagc agggtctatg
    11461 tgaactaaaa ttcgcttagt gacgagcgcg caccagctgg tacttacgtg tcagatactc
    11521 caccggtacg ctccagatgt gtaccagacg cgagaacggg aacagcagga ataacgtcat
    11581 ccccagtacc aggtgtagac ggaagataaa cgccacacca tcgaggtgtt gagaagcgcc
    11641 accgtggaag gtcaccaccg actgcgccca gccaaccagt ttcatcatct cgctaccgtc
    11701 catatgttgg gcggagaacg gaatggtcaa caggcccagc gcgcactgaa taacgagcag
    11761 cgacaggatc aggatatccg ctccggtagt ggttgcacgc acgcgtgggc tgaacagacg
    11821 acgcttaagc aacagcacgc cgccaatcag acacagcacg ccgctggcac caccagcaaa
    11881 catcgccatt ttctgtttca cttcaatcgg cagccaggct tcatacatcc agtgcggcgt
    11941 caacataccg aagaagtgac cgacaaaaat ccccagaata ccgatatgga acaggttcga
    12001 cgccaggttc atccctttgc gatccagcat ctggctggac gccgcgcgcc aggtgtactg
    12061 cccgtagtca taacgcagcc agctaccaat caggaagacc gccccggcta tgtacgggta
    12121 gatatcaaag aagaacatat tcaggaattg cattagtgct gtcctccggt ggtgatattc
    12181 agatattgcg gcgcgacggc accggcaaag cgacgctgat gagcagtgat tgctgaatca
    12241 ccgcagcctt tgtcagcaaa gaatttaacc tgctcttctt cccagacagc atccagcgcc
    12301 tgcggcgtat cgtcgcgcgc ttcgtcggca attttttccg ccactttgtc gctgtcgata
    12361 gcggtattcg ccagtttcat cagcagatca aacagcacgg cataacggct ttcacgctgt
    12421 tgcagacgcg cgctcagcaa tgccagaatc ggcgcgatat ctttcaaacc ttccacggct
    12481 tcgctttgcg gtagctgcgc caggtactcc agatacagcg gcagatggtc cggcaattcg
    12541 cggctgttta actgcaagcc gtgctgctcg tactgcgcca gcaggtccac catcgcctgg
    12601 ccgcggtcgc gggattcgcc gtgcacatgt tcaaacagca acagtgacgt ggcgcggcca
    12661 cggtcgaaca gttcgctgta ctgcgcctgg gcatcgagcg gatccatcgt cgttaaatcg
    12721 cgcaggaaaa tgcccagcgc atgggcatcc tcttttggca gatttttcga cgcggcaatc
    12781 gcctcaaaca tctcttgttg atactgccat aaggcagcat ccggatattc aaggagacgc
    12841 gatacaatca cgagttcgat catgggtgcg gctccgtttt gctggtcaca tcgatggcat
    12901 cgatacgacg gctgttgaac agattgaatt tggtatctga accgtggcag ccatcaccaa
    12961 aggtaaagcc gcagccattt ttctccggga aggcttcccg tgccagttca cgatgactac
    13021 tcggcaccac aaagcgatct tcgtagttag caatcgccag ataacggtac atctcctgtg
    13081 cctgggcttc ggtcagaccg acctcttcca gcgcacgggt atccactttc ccgtcaacgg
    13141 tttcagcacg tttgtaatga cgcatcgcca gcatacgttt cagcgcgcgc agtaccggtt
    13201 tggtatcacc ggcggtcagc agattcgcca gatactgtac cggaatacgt aggctttcga
    13261 cgtctggcag aatgccgttg ctacccagct cacccgcgtc tgctgcagac tgaatcggag
    13321 acagaggcgg cacgtaccag accatcggca gtgtgcgata ttctggatgc agcggcagcg
    13381 ccagtttcca ttccattacc attttataaa ccggcgactg ctgtgcagct tcaataacgc
    13441 tcagcggaat accgtcttta atcgcctgct cgatgacttt cggatcgttc ggatcgagga
    13501 acacctccag ctgacgctgg tagagatctt tctcgttctc ggtgctggct gcacgttcaa
    13561 tcgcgtcggc atcgtacagc agcacgccaa gataacggat acgaccgaca caggtttctg
    13621 agcacacggt cggctgaccc gcttcaatac gcggatagca gaagatgcac ttctcagact
    13681 taccgctctt ccagttgaag tagatttttt tgtatgggca tccggtgatg cacatacgcc
    13741 agccacggca tttatcctgg tcgatcagga cgatgccatc ttcttcacgc ttgtaaatcg
    13801 caccgctcgg gcaggtcgcc acacatgccg ggttcaggca gtgttcgcac aggcgcggca
    13861 aatacatcat gaaggtgttt tcgaactggc tatacatcgc cttctggatg ttgtcgaagt
    13921 tcttgtcttt cgccagtttg tcaaactcac cgcccaggtc atcttcccag ttcggccctt
    13981 tttcgatttt cgccatccgt tccccggtaa tcagcgaacg cggacgggca atcggctgcg
    14041 atttgctgcc ttccggcgcg gtatgcaggt tctgatagtc aaaatcgaac ggctcgtaat
    14101 aatcgtcgat ccccggcaga tgcgggttag cgaagatttt acccagcagc atggcacggt
    14161 tacccatgcg cggctgcagt ttgccgttga ttttacggat ccagccgcct ttgtattttt
    14221 cctggttttc ccagtcagtc gggaagccct ggcccggctt ggtttccacg ttgttgaacc
    14281 acgcgtattc cacgccttca cggctggtcc agacgttttt acaggtaact gaacaggtgt
    14341 ggcagccgat gcacttatcg agattcagca ccatgccgac ttgtgaacga attttcattt
    14401 tacgctctcc tgtacctggt cattgccttc gccatctaac cagtcaatgt tcttcatctt
    14461 acgcactaca acaaactcat cgcggttaga acctacggtg ccgtagtagt taaagccgta
    14521 tgccagatgg gcatagccgc cgatcatatg cgtcggtttc ggcgtgatac gggtgaccga
    14581 gttatggata ccaccacgct gttgggtaat ttccgaacca ggcaggttaa cgatacgttc
    14641 ctgcgcgtgg tacatcatgg tcatccctgc cggaacacgc tggctgacaa ccgcacgggc
    14701 agtcagagca ccgttgctgt tgaatacttc aatccagtcg ttatcggcga tacccagatc
    14761 tttggcatcg gcttcactca accagaccac cggaccaccg cgacctaaag tcagcatcag
    14821 caggttgtcg ctgtaggtgg agtggatacc ccacttctgg tgcggcgtca ggaagttgag
    14881 cgctttttcc gggttgccgt tggatttctg gcctatcact tctttcaccg aacgggtgtc
    14941 gatcggcgga cgataaacca gcaggctttc accgaaatca cgcatccact ggtgatcctg
    15001 atacagttgc tgacgaccag agagcgtacg ccatgggatc agctcgtgaa cgttggtgta
    15061 accggcgttg taagaaacgt gttcatcttc cagaccagac caggtcgggc tggagataat
    15121 tttgcgcggc tgtgcctgaa tatcgcggaa gcggatcttc tcgtcttctt tattcagcgc
    15181 cagatgcgta tggtcacgac cggtaaactc gctcagggca gcccaggctt tcacggctac
    15241 ctgaccgttg gtttccggtg ccagcgtcag gatcatctct gccgcatcaa ttgcggtgtt
    15301 cagcatcggc tggcctttcg ccggaccttc cgctttggtg tagttgagct tacgcagcag
    15361 atccatctcg ctctgggtgt tccaggcaat ccctttaccg ccgttaccga ttttctccat
    15421 cagcgggccg atagaggtaa agcgttcgta agttgccgga taatcgcgct ctacgaccat
    15481 aatgtgcggt gcagttttgc ctgggatcag gtcgcattcg ccttttttcc agtctttcac
    15541 atccagcggc tgcgccagtt cagcggcaga gtcgtgctgg ataggcagcg tgacgatgtc
    15601 ggtttcttta cccagatggc cgacgcacac ttcggagaat ttcttcgcga tggctttgta
    15661 gatttcccag tcgcttttcg cttcccaggc cggatcgacc gccgcagaca gcgggtgaat
    15721 aaacggatgc atatccgaag tattcatgtc gtctttttcg taccaggtcg ccgtcggcaa
    15781 aatgatgtcg gaatagagac aggtgctcga cagacggaag tccagcgtaa ccaccagatc
    15841 cagcttgcct tccagaccat tgtcctgcca gtccacttct tccggcttca cgccgccctg
    15901 ctgccccaga tctttaccct ggataccgtg ctccgtcccc agcaggtact tgagcataaa
    15961 ctcatgacct ttaccggaag aaccgagcag gttagaacgc cagatgaaca ggttgcgcgg
    16021 gtggttttta ccgttttctg gttgttctgc cgcaaaacgg atggaaccct ctttcaggga
    16081 tttcaccgta tagtccaccg gattcatccc ggctttttcc gcttcgccag cgatagtcag
    16141 cgggttagtg cctaactgcg gtgcagacgg cagccagccc atgcgttccg cacggacgtt
    16201 aaagtcgatc aggtgtccgg tataacggga tttatccgcc atcggtgaca gcaactcttc
    16261 cgccgtgacg gtttcataac gccactggct ggagtggtta tagaaataag aagtgctgtt
    16321 catgtgacgc gccggacgct gccagtcaag ggcaaacgcc agcggctgcc agccggtttg
    16381 cggacgcagt ttttcctgac ctacatagtg cgcccagccg cccccgctct gaccgacaca
    16441 gccgcagaaa atcagcatgt tgatcagacc acgatagttc atatcgaggt gataccagtg
    16501 gttcagcccc gcaccgacga taatcatcga acgaccgtgc gttttatcag cgttatcggc
    16561 aaattcacgg gcgatgcgaa taatctggct gcgagaaacg ccggtaatct gctcggccca
    16621 ggccggggta taagctttca catcgtcata gctggttgca cagttaacgt cgttcaggcc
    16681 acgttccaga ccgtagtttg ccagcgtcaa atcataaacg gtggtcacca gggcggtgct
    16741 gccatcagcc agttgcaggc gtttcaccgg cagtttgtgc agcagcacgt tttccagttc
    16801 cactttgttg aagtgttccg tgccgtcgcc accaaagtac gggaagccta cctcagcgat
    16861 ctcatcctga ctacccagca ggctcagttg cagctcggtt tcttcgccag ttttgccgtc
    16921 gcgctgctca agattccatt tgcccttctc gccccagcgg aagccaatag aaccgttcgg
    16981 cgcaaccatt tcgccattgg tattaaaggc gacagttttc cattccggat tgttttcctg
    17041 gcccagcgca tcaaccagat cagcagcgcg cagcatacga cctgcagcgt aataaccgtc
    17101 gcgttcttcc agcatcacca gcatcggcat gtcggtgtag cgacgcacat agtcggtgaa
    17161 atactggctt gggttgtcga ggtggaattc acgcagcatt acgtggccca tcgccagcgc
    17221 cattgccgca tcggtgccct gtttcggtgc cagccacaga tcgcacagtt tggcgatttc
    17281 agcgtagtct ggtgtgacgg caacagtttt ggtgcctttg taacgcactt cagtaaagaa
    17341 gtgagcatcc ggggtacgcg tctgcggcac gtttgaaccc caggcgatga tgtagctgga
    17401 gttgtaccag tcagcagatt ccggtacgtc agtttgctcg ccccaggttt gcggagacgc
    17461 aggaggcagg tcgcagtacc agtcgtagaa gcttaagcaa gtaccgccaa tcagcgagag
    17521 atagcgtgca cccgatgcgt aagaaaccat cgacattgcc ggaattggcg agaaaccagc
    17581 aacacggtcc gggccgtagt ttttgatggt gtaaacgtta gatgcggcga tcagttcgtt
    17641 cacctcctgc caggaagaac gaacaaaacc accgcgtcca cgcgcctgct taaagctttt
    17701 cgctttatcg gcgtcttcaa tgatagaagc ccatgcctca accggatcgc tatgcagcgc
    17761 cttcgcttca cgccacattt tcatcaggcg tttgcgcatc atcgggtatt tcaggcggtt
    17821 ggcactgtaa agataccagg agtagctggc accgcgcggg cagccgcgag gttcatggtt
    17881 tggcagatcc ggacgggtac gcggatagtc agtctgctgg gtttcccagg tgaccagacc
    17941 gtttttgacg tagattttcc agctgcagga gccggtgcag tttaccccgt gggtagagcg
    18001 gacgattttg tcatgctgcc aacgctggcg atatccatcc tcccagtcac ggttggtatt
    18061 gagaagctgg ccatgcccat cggcaaaggt ttcacccttc tgtttgaagt agcgaaaccg
    18121 gtccaggaat ttactcatcg gttttctcct gtgggagcct gtcggccctc tgataaatcg
    18181 acattgctaa tgtttaaggg caaggtaacg ctctgaaaca gcgtgggaat tgataacgat
    18241 caagagtgat ggggatgaaa aataaagtaa attcctaaga taccccttta ggataattaa
    18301 cag
//
LOCUS       DAZKFU020000081        18235 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZKFU020000081 DAZKFU020000000
VERSION     DAZKFU020000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.081"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(43..801)
                     /gene="aroD"
                     /locus_tag="ACHOQQ_RS11030"
                     /old_locus_tag="pgaptmp_002206"
     CDS             complement(43..801)
                     /gene="aroD"
                     /locus_tag="ACHOQQ_RS11030"
                     /old_locus_tag="pgaptmp_002206"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGU4470448.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(832..1698)
                     /gene="ydiB"
                     /locus_tag="ACHOQQ_RS11035"
                     /old_locus_tag="pgaptmp_002207"
     CDS             complement(832..1698)
                     /gene="ydiB"
                     /locus_tag="ACHOQQ_RS11035"
                     /old_locus_tag="pgaptmp_002207"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGU4470449.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1710..2975)
                     /locus_tag="ACHOQQ_RS11040"
                     /old_locus_tag="pgaptmp_002208"
     CDS             complement(1710..2975)
                     /locus_tag="ACHOQQ_RS11040"
                     /old_locus_tag="pgaptmp_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4470450.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3202..4416)
                     /locus_tag="ACHOQQ_RS11045"
                     /old_locus_tag="pgaptmp_002209"
     CDS             complement(3202..4416)
                     /locus_tag="ACHOQQ_RS11045"
                     /old_locus_tag="pgaptmp_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4470451.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4516..4872)
                     /locus_tag="ACHOQQ_RS11050"
                     /old_locus_tag="pgaptmp_002210"
     CDS             complement(4516..4872)
                     /locus_tag="ACHOQQ_RS11050"
                     /old_locus_tag="pgaptmp_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGU4470452.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5008..5115)
                     /gene="rprA"
                     /locus_tag="ACHOQQ_RS11055"
                     /old_locus_tag="pgaptmp_002211"
     ncRNA           complement(5008..5115)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHOQQ_RS11055"
                     /old_locus_tag="pgaptmp_002211"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5301..6413)
                     /gene="ydiK"
                     /locus_tag="ACHOQQ_RS11060"
                     /old_locus_tag="pgaptmp_002212"
     CDS             complement(5301..6413)
                     /gene="ydiK"
                     /locus_tag="ACHOQQ_RS11060"
                     /old_locus_tag="pgaptmp_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGU4470453.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6802..9858
                     /locus_tag="ACHOQQ_RS11065"
                     /old_locus_tag="pgaptmp_002213"
     CDS             6802..9858
                     /locus_tag="ACHOQQ_RS11065"
                     /old_locus_tag="pgaptmp_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGU4470454.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLMR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9855..10265
                     /gene="menI"
                     /locus_tag="ACHOQQ_RS11070"
                     /old_locus_tag="pgaptmp_002214"
     CDS             9855..10265
                     /gene="menI"
                     /locus_tag="ACHOQQ_RS11070"
                     /old_locus_tag="pgaptmp_002214"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGU4470455.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGYDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10365..10553
                     /locus_tag="ACHOQQ_RS11075"
                     /old_locus_tag="pgaptmp_002215"
     CDS             10365..10553
                     /locus_tag="ACHOQQ_RS11075"
                     /old_locus_tag="pgaptmp_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGU4470456.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11102..11470
                     /gene="sufA"
                     /locus_tag="ACHOQQ_RS11080"
                     /old_locus_tag="pgaptmp_002216"
     CDS             11102..11470
                     /gene="sufA"
                     /locus_tag="ACHOQQ_RS11080"
                     /old_locus_tag="pgaptmp_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGU4470457.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11479..12966
                     /gene="sufB"
                     /locus_tag="ACHOQQ_RS11085"
                     /old_locus_tag="pgaptmp_002217"
     CDS             11479..12966
                     /gene="sufB"
                     /locus_tag="ACHOQQ_RS11085"
                     /old_locus_tag="pgaptmp_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGU4470458.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12976..13722
                     /gene="sufC"
                     /locus_tag="ACHOQQ_RS11090"
                     /old_locus_tag="pgaptmp_002218"
     CDS             12976..13722
                     /gene="sufC"
                     /locus_tag="ACHOQQ_RS11090"
                     /old_locus_tag="pgaptmp_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGU4470459.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPGLCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13697..14968
                     /gene="sufD"
                     /locus_tag="ACHOQQ_RS11095"
                     /old_locus_tag="pgaptmp_002219"
     CDS             13697..14968
                     /gene="sufD"
                     /locus_tag="ACHOQQ_RS11095"
                     /old_locus_tag="pgaptmp_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGU4470460.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGVKRSPQAQQHLEQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSMLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14965..16185
                     /gene="sufS"
                     /locus_tag="ACHOQQ_RS11100"
                     /old_locus_tag="pgaptmp_002220"
     CDS             14965..16185
                     /gene="sufS"
                     /locus_tag="ACHOQQ_RS11100"
                     /old_locus_tag="pgaptmp_002220"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGU4470461.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLEMLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPEDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16198..16614
                     /gene="sufE"
                     /locus_tag="ACHOQQ_RS11105"
                     /old_locus_tag="pgaptmp_002221"
     CDS             16198..16614
                     /gene="sufE"
                     /locus_tag="ACHOQQ_RS11105"
                     /old_locus_tag="pgaptmp_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGU4470462.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16763..17767
                     /gene="ldtE"
                     /locus_tag="ACHOQQ_RS11110"
                     /old_locus_tag="pgaptmp_002222"
     CDS             16763..17767
                     /gene="ldtE"
                     /locus_tag="ACHOQQ_RS11110"
                     /old_locus_tag="pgaptmp_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGU4470463.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17831..18067)
                     /gene="lpp"
                     /locus_tag="ACHOQQ_RS11115"
                     /old_locus_tag="pgaptmp_002223"
     CDS             complement(17831..18067)
                     /gene="lpp"
                     /locus_tag="ACHOQQ_RS11115"
                     /old_locus_tag="pgaptmp_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGU4470464.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4779 a         4405 c         4705 g         4346 t
ORIGIN      
        1 ttagttcggc gggggaggat attcccgccg aaatattatt gcttatgcct gatgtaaaat
       61 agttaatact gtgcgcaaat catttaccga gatttgccct ggcgcagagg ctttttttac
      121 cgcaccaaaa gttgccgccg acccaaatac ttcaccagcc agacgagaaa ttacgccagt
      181 ttttgccatt gacatcgtga tgattggacg atcggcatac tgctcctgca tctccagggt
      241 cgcggcaagc aacgtcagca catcgctggt actttgcggc atcagcgcaa tcttaggaat
      301 atcggcgtcg aaggactgca ttttgcgcag acgggcaatg atttcttcgg cttccggcgt
      361 tttatggaag tcatggttgg acatgacaac tttcacatca tgcgcgtggg cgtaggcgac
      421 ggtttcttta acctgatcat cgccggtaaa taactccaga tcgatcatat caaccaggcc
      481 gctgtcgatg gctgcacgat tgagagcaat ataagcctcg gtggaaatcg cctgctcgcc
      541 gccttctttg gcactgcgga aggtaaacag cagcggtttt tctggcatgg tttcacggag
      601 aatttttgcc gccgccatga cagactccac attggagagg tcggcaaagt ggtccacacg
      661 ccattccaga atatcaaagt ccgcttcacg ataggcgaga gcttcggatt tcacgcgggc
      721 gatatctttc gccatcagcg agacgatgat tttaggtgcg cccgtaccaa tgacgagatc
      781 ttttacagtt acggttttca ttttttaccc tttctgcacg cggtcagcct gtcaggcacc
      841 gaaccccatg acctgtttaa catattccag agggaaatct ttgccagtcc ataacgtgaa
      901 ctgttcagcc ccttgccaca acaacatgcc gtatccatca atcgttttgc aaccagcttg
      961 ttgcgcctgc tgcaataact tcgtcatatg cgggttatac acgcattcag tgaccagaag
     1021 tcccggatgt aacagactga tatcattaac caatgattca ttctcaaggg gtttcatacc
     1081 cacttttgtg ccattggtta aaatgtcggc ggaagccagg gcttcagcaa aggcttgctg
     1141 atcggcgaga tcggtaaccg tgacgacaca atcggtgttt tcgttaaccc gctgcgcgaa
     1201 ggcgagggct ttatcgaaga actcatcccg acggttaaag agtttaattt cttttaaacc
     1261 ttcaattgcc ccctgcgcgc caatcgccgt tgaggcacct ccggccccta acagcaccat
     1321 tgttttgcct ttgatatcaa aaccgctctc tttaatggcg cgaatatggc ccgtgccgtc
     1381 ggtgttatag ccgcgcaggt agccatcatc attaacgatg gtgttgatgg cacccaccag
     1441 tttggctgcc ggcgttaatt catcaacata ttcacacgcc agttgtttgt tcggcattga
     1501 tacaccagtt ccgcgcattt ttagagcttt taatccttca attgctccgg gaaagctatc
     1561 gttatccact tcgaaggcca tataggtaaa tggcaatccc gctttttcta aggctttatt
     1621 ctgcatttca ggcgataaac tgtggcggat aggataagcc atcaacccaa tcaattcgta
     1681 ttttgcggta acatccataa ttaactcctt taacctctat gcttaattgt gtttaaccgg
     1741 gtgctaaatt tgcgctcgcc aaaccgcaca tcattctcag gaataataaa aacgcggtaa
     1801 tagcggataa aaacaataat tgcggtaatc agggccagca gcgcgaaagt aaaatcgaga
     1861 acaatgatat attgcaggcc gatgttcgac agataaccgg taatcagcgg aataacaaag
     1921 ttagccagtc cacccatcat catataaata ctggtgactt tggctttgct ttttgggaaa
     1981 aactctgaca tgaccgaaac accgagctgt aaaatgccgc cagctgctga gaaaccgata
     2041 acaaaggcgc cggcattaca caccagtgga gaagggtaca ggtagataat ggctgctgtt
     2101 attgttgcca gtgcagagtt aaatacatta gcccagatgg gccggaccat ttttttcagt
     2161 agtgcggcaa aaataaagac acagaccaac gagcccatac tgtaataaga gatggttttt
     2221 aatgcctcag cttctgacat accagcaaaa gccatcgcat atttgggcat ccacaccaca
     2281 atcacataaa aggtcgagaa tgcggctaca ccgaacagta ccgatgaaac accttccagc
     2341 cagactaacg gtttgctgtt catttgcggt aattcattag ctacgctggc gtccaccaac
     2401 tggctgggga atttgctttt caacagcatc agcgtgatca gtacaaacag aatacccgga
     2461 ataatcagcc catagccgta ccagatatta ttgagcaaca tatagctcac cagcattggg
     2521 tagaacattt gcccaaatga caccatcgct ttaaccagta tgaccgccga accagaggct
     2581 ttcggaaagc attccatgag cgcggggtag ccacccgtat ccagcgctga gttagcgata
     2641 cctacacaca ctgccagacc gtaggcgaga gttaaattcg ggcaagcggg aataccaaag
     2701 aagaatagca gatacattat tactgccatt aatatcaccg cccgacgacc aaacttatcg
     2761 gagatcacac cgaagaataa aatactgatc aatcgcccca aaccgatacc ggaaattaag
     2821 taggcaatgc ccgcgttgtc agtggaaaac ttttccgcca gagatgacat attttgggca
     2881 agcgtaataa cactaatgcc gtgcaggaag tagctgaagt aaatacaaag aacagccagg
     2941 ataaatggcg tgctgaaagc cttattttga gacattttca cagctccttg ctgaaagata
     3001 atttcgttac cgtacgtatt gttctccgac ggtttacata attactatac tgaagatgat
     3061 tttccctggg tacatggcat aaagtttatg tttccaggaa tattgcatgt cataatttgt
     3121 ttttgatcgc gattttcctt agttagcgca gaaagagtta tttatccgta agatcttgca
     3181 tgacctgaaa agaatattgc attacccacc cggagcgaca ttttccttta gcgagtgatg
     3241 ccgcgttttt ttgcggctgc gtaagccgat aaacagtgcc agtaaaaagc cgatggcagc
     3301 gatggcggta tcgaaccaca taatatcggc aatacttctt tgggacagat gagccgtaat
     3361 caacggaata gtaaaggtcg caatactgcc cgcactgtaa tagatccctg tagctttacc
     3421 tttagcgtag gggaaacgtt cagccattaa cgtcaggcca atttgcacaa cacctccagc
     3481 agaggtaaaa ccaatgacaa aagcaaatat tatcaccaca taaaatgtgg gatgcaggca
     3541 gacggtaaac agagcaataa atgagataaa ggtgtacagc atcagtaatg ttgttgggcg
     3601 aacggtatta cgaatgagtg gagcggtaat aaatacacaa agcagcgaac ccacggtgta
     3661 gatactgagt agtttgatcg acatagtgta tgacatgcct gcaacaaatt gtccgtactg
     3721 tgccagccac tggctaacca gataaaacgt tgccattgag atatagccat ataaggtata
     3781 actggctaaa tcgataattg aacagcgatg ttccgtagag ctggtggttt tctttatgac
     3841 aggtaagcga cgacccggat ggggggggaa cgtacaacgg tataaaaaca gagcgttaat
     3901 aaacataatg cctgcagcaa tcataaagga ccaaccgaac cacagttcag cccacactaa
     3961 caggctaatg attagcggta ataaaaattg tccgctggaa acaaatgctt taattaaaat
     4021 attggctgtc ccaggtgagc gtggaaaagc ttccatcaaa ctgggataag tgcctgcatc
     4081 gagaaaactg tttgccattc ccgccagaaa gccaaaaaca taagcgataa tgatgttatt
     4141 ggtctgcagg atgccaaaaa agaaggccat atagcagcac atcccgagca tgataaaagg
     4201 gcggcgacca aagcgatcgg ataataatcc tgcaaaaagc aagacactta atcgaccaat
     4261 gcccagcgat gagataacta tcgagacacc cgcggcatta gtctgccaaa gtgtctccag
     4321 cgaggccata ttcaggctca tcaaaatgac gcccatacca tgcaccaggt aattaaaata
     4381 caacccaagt gcggtagggt aatagggatt tttcataggt tctacttagc aaaggtgctt
     4441 taaaaagtgt agcgattttg tgtcagtatc cgttcattat tgttgtaaca acgacaattc
     4501 ctttttgtgg agaaattaac aaagccgctc cagatcgtgt gcatataatt cagcggctac
     4561 cgattgatag atcttccagt caataaaatt gccatccggg tagacacgtt gaaacgcggt
     4621 taagtcgggg aaaaaacgca tggtgttgtt gccgatgcgg ttatttattt tctctataat
     4681 ggcatgaaga tgtttttttc gttgctgacg catagccaac agttgctcga ctacacgatc
     4741 aggaatggcg catttgccat tttcccactg acgccagctt tcactgtcac cggtctgggc
     4801 aatccaggtc gcgcattcat caatagtcat ggcaaaaata tggcggagtg cctggagttc
     4861 ataagcgttc atcgtgtaat ggctgcatga aataaacaat aaaatataaa taacattatc
     4921 agcctgctga cggcttgaag agagtcacag tatcttgtgc aacattattg gaaagagtga
     4981 ggggcgaggt agcgaagcgg aaaaatgtta aaaaaaagcc catcgtggga gatgggcaaa
     5041 gactacacac agcaattcgt tgtttcactc aggggatttc catgcttata aatcaatatg
     5101 ttgatttata accgtgagct aatagtaggc atacggactt tttgcgtcga tcagattcgt
     5161 ctcattagtt taaaaactgt aaagaatttg cgacaggtac taccagctga atggcggtaa
     5221 gcaggaaaat atggggaaga gcaagcaaat atcaattagc ggtggttaac aatcaggcgg
     5281 cagagccgcc tgatgagaaa ttacttattc ggtttctcga tttcgccaag ttcttcgaga
     5341 atttcttccg gttggtccgt cggcggcggg acttcttcca cccacgcggc aaacagacgc
     5401 caggaaacgg ctaacagaac cggaccaata aacagaccga tcatcccgaa agcaatcaaa
     5461 ccaccaataa cgccagagag gatcaggatc agcggtaaat cggcacccat gcgaattaac
     5521 attgggcgga tgacgttatc cagcgtgcca accacaccgc tccacactaa caataccgtt
     5581 ccccaggtgg tatcgccggt ccagtagagc cagataatcg ccggaatcag taccggcaac
     5641 gggccaagct ggacaaggca ggagaggatc attaacaccg ttagcaacgt tgcataaggt
     5701 acgccggata cggcgaggcc gataccgcca agcactgcct gtactaacgc cgtcaccacc
     5761 acacccagcg ccaccgcgcg gatagcctgt gccgccagca gcacggcggc atctccgcga
     5821 acgcctgcca gacgggtcgc aaaatggcga atgccttgtg ccacctgttc accgcgccag
     5881 tacagcaggg cactgaagag aagcatcagc gcacaatgca ccataaagcg cccgatatgc
     5941 gccgcttgcc caacgaacca ggtggtggtg gtgccaatat aagggcggac tttcgccatg
     6001 atcgccgtcc cccccatatc cagcaagttg tgccagcctg catacagctt cgcgccaatc
     6061 accggaatgg tattaagcca cgccagatcg ggtaacgtca tgtcaccgct ggaaatggct
     6121 ttaattagcg ggccgctgcc gtcgacgata ctgttaacca gcaaagcaat agggatgata
     6181 aacaccatca ctaataacag cgtcatcacc agaacggcga gagagcggcg gccaaacatg
     6241 atcttttgca aacgtaacaa taccggccag gtggcgataa ccaccgtacc ggcccatgca
     6301 aagccgagaa taaagggttg aacaatccac agacatgcca caatcatgat ggctaaaaac
     6361 agcaccgaaa gcagaatttg tgcgacatcc ctgggctgac gaacatttac cataactact
     6421 tttcaccttt tttgtgcgtc aaatcgtcga cgcgacgtga acacgcgaaa ctcacctgca
     6481 taatgatgag caatttcagc ggtttttaac aggccgattc tgcccttaat tctgatgggc
     6541 gtgttagaaa aaaatgtggt acaacaactc aggcaacacg caaacgattt actcgtcgta
     6601 tttcaagttg catatgctgc ggctacgttc gttcacccca gttacttact gttgtaagct
     6661 cctggagttc actcgcttgc cgccttcctg caactcgaaa tccatagagt aaataaacat
     6721 cacctttctg tgcgctattg cagccgcctg aaagaacagt gtatcgacct acatcacaga
     6781 ccgcaggaaa gggtcaatat aatgattcca cagatttccc aggcacctgg cgtcgttcaa
     6841 ctggtgctta attttttgca agagctggag caacaaggtt ttaccggcga tacggcgaca
     6901 agttatgccg atcgtctgac aatgtcgacc gacaacagta tttaccaact tctccccgat
     6961 gcggtggtat ttccgcgttc aaccgcagat gtggcgctga tcgcccgtct tgccgcgcag
     7021 gaacgctatt catcgctgat ctttaccccc cgcggcggcg gcaccggcac taacggtcag
     7081 gcgctcaacc agggcattat tgttgatatg tcccgccata tgaaccgcat catcgaaatt
     7141 aaccctgaag agggctgggt gcgcgttgag gccggggtga taaaagacca actcaatcag
     7201 tacctgaaac cgttcggcta cttttttgcg ccggaacttt cgaccagcaa ccgggcaacg
     7261 ctcggtggga tgatcaatac cgatgcatcc ggtcagggat cgctggtcta tggcaaaacg
     7321 tcagatcacg tacttggcgt acgcgcggtg ttgttggggg gcgatattct cgatacgcaa
     7381 cctttacccg tcgagctggc ggaaacgctg ggtaaatcca ataccacaat cgggcgaatt
     7441 tataacacgg tttatcaacg ttgccgtcag caacgccagt taattatcga caacttcccc
     7501 aaacttaacc gctttcttac cggttacgat ctgcgtcatg tctttaacga tgagatgacc
     7561 gagttcgacc tgacgcgcat tctgacgggt tcagaaggga cgctggcctt tattaccgaa
     7621 gcgcggctgg atattacgcg cttgcctaaa gtgcggcgtc tggtgaatgt caaatatgac
     7681 tcttttgact ctgcgctgcg taacgcgccg tttatggttg aggcgcgggc gctttcggta
     7741 gagacggtgg actcaaaagt gctgaatctg gcgcgggaag atattgtctg gcattccgtc
     7801 agcgagttga ttaccgatgt gcctgacaaa gagatgctcg ggctgaacat tgtggaattt
     7861 gctggtgatg atgaggcgct gattgatgag cgggtaaatg cactctgtgc gcggttggat
     7921 gagctgatcg ccagtcatca agcaggtgtc atcggctggc aggtgtgccg tgagctggcg
     7981 ggggttgaac gtatctatgc aatgcgcaaa aaagccgttg gtctgcttgg caatgccaaa
     8041 ggtgccgcta agccaattcc gtttgctgag gatacctgcg taccgccgga acatctggcc
     8101 gattatattg ctgaatttcg cgcgctgctc gacagccacg gcttaagtta tggcatgttt
     8161 ggtcatgtcg atgctggggt gttgcacgtc cgtccggcac tggatatgtg cgatccgcaa
     8221 caagagattt tgatgaagca aatttctgat gacgtggtgg cgctgactgc gaaatacggt
     8281 ggtttgttgt ggggcgagca cggcaaaggt tttcgcgctg aatacagccc ggcgtttttc
     8341 ggtgaggaac tttttgcaga actgcgcaaa gtgaaagcgg catttgaccc gcataaccga
     8401 ctcaacccag ggaagatttg cccgccagaa ggtctcgatg cgccgatgat gaaagtggac
     8461 gcggtgaagc gcggtacatt cgatcggcag atccccattg cggtacgcca gcagtggcgc
     8521 ggtgcgatgg agtgtaacgg caacggttta tgcttcaact ttgatgcccg tagtccgatg
     8581 tgtccgtcga tgaagatcac ccagaaccgg attcattcac cgaaagggcg cgcaacgctg
     8641 gtgcgtgaat ggctgcgttt gttggcggat cgcggcgttg atccactcaa actggaacaa
     8701 gaactgcctg aatccggcgt cagtttgcgg acgttaattg cccgcacgcg caatagctgg
     8761 catgcgaata aaggcgaata tgacttctca cacgaagtca aagaggcgat gtcgggctgt
     8821 ctggcctgta aagcgtgttc gacccagtgc cccatcaaaa ttgatgtgcc ggagtttcgc
     8881 tctcgtttcc tgcagctcta tcacacccgt tatttacgtc cgctgcgcga ccacctcgtc
     8941 gctacggtcg agagctacgc accgctgatg gcacgcgcgc cgaaaacctt taacttcttc
     9001 attaaccagc cgctgatgcg caaactctcg gaaaaacata tcggcatggt tgatttgccg
     9061 ctgctgtcgg tcccctcgct acaacaacaa atggtggggc atcgctcggc aaacatgacg
     9121 ctggaacagc ttgaagctct caatgcagag cagaaagcgc gcacggtgtt ggtggtgcag
     9181 gaccccttta ccagctatta cgatgcgcaa gtggtggcgg attttgtccg tctggtcgaa
     9241 aaattaggtt tccagcctgt gttactgcca ttttcgccaa atggcaaagc ccagcatatt
     9301 aaaggttttc ttaatcgttt tgcgaagacg gcgaaaaaga cggcggattt cctcaaccgt
     9361 atggcgaagc tgggtatgcc aatggtaggc gtcgatccgg cgctggtact ttgttatcgc
     9421 gatgaatata aactggcact gggcgaggaa cgtggcgagt ttaacgtctt actggcgaat
     9481 gaatggctgg caagcgcact tgagtcacag ccagtggcta cagtcagcgg tgaatcatgg
     9541 tatttctttg gtcactgtac cgaagttacc gccttgccgg gtgcgccagc acaatgggcc
     9601 gcgatatttg cccgttttgg cgcgaaactg gaaaatgtca gcgtgggttg ctgcggcatg
     9661 gcagggactt acggacatga agcgaaaaac cataaaaatt cgctcgggat ctatgagtta
     9721 tcctggcatc aggctatgca gcgactgccg cgtaaccgct gtctggcgac cggatattcc
     9781 tgccgtagtc aggtcaaacg ggttgaaggc acgggggtac gccatcctgt gcaggcttta
     9841 ctggagatta ttaaatgata tggaaacgaa aaatcaccct ggaagcactg aatgctatgg
     9901 gtgaaggaaa catggtgggg ttactggata ttcgctttga acatattggt tatgacaccc
     9961 ttgaagcgac aatgccagta gactcgcgga caaagcagcc tttcgggttg ctacatggag
    10021 gtgcatccgt ggtactggcc gaaagtatcg gttccgttgc cggttattta tgtaccgaag
    10081 gtgagcaaaa agtggttggt ctggaaatca atgctaacca cgtccgctcg gcacgagaag
    10141 ggcgggtgcg cggcgtatgc aaaccgttgc atctcggttc gcgtcaccag gtctggcaga
    10201 ttgaaatctt cgatgagaaa gggcgtttgt gctgttcgtc acgattgacg accgccattt
    10261 tgtgacgttg tcaccagaaa agtgtgacgc atattgcttt tggtcattat ctgcacagta
    10321 aacggtggta tactgtgcag gtcttgtcta aaagcgagga tgctatgtct actcaacttg
    10381 atcccaccca gctggccatt gaatttttac gccgtgacca gagcaatctt tcacctgccc
    10441 aatatctcaa acgtctgaaa caactagaac ttgaatttgc tgacctgctt actctctctt
    10501 ctgctgaatt aaaagaagaa atctactttg cctggcgtct gggcgtgcat tgatcctgac
    10561 ttaatgccgc aagttcgcca aatcgacctg gattaacctc cgtccaggtt cgatgacctt
    10621 cttaatactg cttatctacc cgcctgatat tgttcaaaaa taatactaat cgcagtttgt
    10681 gttaaaacgg cgggttagct ttatgagatt attcttatcg ccccttcaag agctaagcca
    10741 ctgagagtgc cggagataag cgccggatgg ggtagaaacc cttaagcctg tgtcgcacag
    10801 gcttaagggt tttcttattt ctgaacgctg cactttttta cctatctctt aaatagtcat
    10861 tttcaatgca actttttctt tttcatcaca gccttaatgc tccttagata ataaccatta
    10921 tctaacaatg agatacctaa ttcttagcgt gcctgttaac ccacacatca gggtctatgc
    10981 ttattaaaag caggcaaaaa gcatgtttta cggtaaagcc cctgcgtttg ctgggttgaa
    11041 ctgataatca ttatcactaa catgctgtta tacgctgaaa gcgatgaagt gaggtaaatc
    11101 gatggacatg cattcaggaa cctttaatcc acaggatttc gcctggcaag gcttaacgct
    11161 gacacccgca gccgctgtcc acatccgcga gctggtggca aagcagccgg gtatggtcgg
    11221 cgtgcgctta ggtgtgaagc aaacgggctg cgcgggcttt ggctatgtgc tcgacagtgt
    11281 tagcgagccg gacaaagacg atctgctgtt tgaacacgac ggcgcgaagc tgtttgtccc
    11341 gctgcaagcg atgccgttta ttgatggcac ggaagtcgat ttcgttcgtg aaggacttaa
    11401 tcagatattc aaatttcaca accctaaagc ccagaatgaa tgtggctgtg gcgaaagctt
    11461 tggggtatag gcggtactat gtctcgtaat actgaagcaa ctgacgatgt caaaacctgg
    11521 accggcggcc cgctgaatta taaagaagga ttcttcaccc agttagccac cgatgagctg
    11581 gcaaagggga taaacgaaga ggtggtgcgc gcaatttcgg cgaagcgtaa tgagccggaa
    11641 tggatgctgg agtttcgtct aaacgcctat cgcgcatggc tggagatgga agaaccgcac
    11701 tggttgaaag cgcactacga caagctgaat tatcaggatt acagctacta ctcagcacca
    11761 tcgtgcggta attgtgacga cacttgcgcg tctgaacctg gcgcggtgca gcaaactggc
    11821 gcgaacgcct ttttaagtaa agaggtggag gcggcgtttg agcagttggg cgttcccgtg
    11881 cgggaaggca aagaggtggc ggtggatgcc attttcgact cagtttcggt tgccactact
    11941 tatcgcgaaa aactggcgga gcagggaatt attttctgtt cctttggtga ggcgatccac
    12001 gatcacccgg aactggtgcg taaatatctc ggcaccgtgg tgccggggaa tgacaacttc
    12061 tttgccgcgc ttaatgcggc ggtagcctct gacggtacgt ttatttatgt gcctaaaggc
    12121 gtgcgctgcc cgatggaact ttccacctat tttcgcatta acgcggaaaa aaccgggcag
    12181 tttgagcgca ccattctggt ggccgacgaa gacagctacg tcagctacat tgaaggctgt
    12241 tccgctccgg tgcgtgacag ctatcagttg cacgcggcag tggtggaagt catcatccat
    12301 aaaaacgccg aggtgaaata ttccacggta caaaactggt tccctggcga taacaacacc
    12361 ggcggtattc tcaacttcgt caccaagcgt gctttgtgcg aaggcgaaaa cagcaaaatg
    12421 tcatggacgc aatcagaaac cgggtcagcg attacgtgga aatatcccag ctgcattttg
    12481 cgcggcgata actccattgg tgagttttac tcagtggcgc tgaccagcgg tcatcagcaa
    12541 gcggataccg gcaccaagat gatccacatc ggtaaaaaca ccaaatcgac cattatctcg
    12601 aaagggatct ctgccggaca tagtcagaac agttatcgcg gcttagtgaa aatcatgccg
    12661 acggcaacca atgcgcgcaa tttcactcag tgcgactcaa tgctgattgg cgctaattgt
    12721 ggggcgcata ccttcccgta tgtcgaatgt cgcaataaca gcgcacaact ggagcacgag
    12781 gcaacgacat cacgtattgg tgaagatcaa ctgttttact gcctgcaacg cgggatcagc
    12841 gaagaagacg ccatctcgat gattgttaac ggtttctgca aagacgtgtt ctcagaactg
    12901 ccgctggaat ttgccgttga agcacaaaaa ctcctcgcca tcagtcttga acacagcgtc
    12961 ggataaggaa taaacatgtt aagtattaaa gatttacacg tcagcgtgga agataaagct
    13021 atcctgcgtg gattaagcct cgacgttcgt cccggcgaag ttcacgccat tatggggcca
    13081 aacggttcgg gcaaaagtac cttatcggca acgcttgccg ggcgagaaga ttatgaagtg
    13141 acgggcggca cggttgagtt caaaggcaaa gatttgcttg cactgtcgcc ggaagatcgt
    13201 gcaggcgaag gcatctttat ggccttccag tatccggtgg agattccagg tgtcagtaac
    13261 cagtttttcc tgcaaacggc acttaatgcg gtgcgcagct atcgcggcca ggaaacgctc
    13321 gaccgctttg attttcagga tttgatggaa gagaaaatcg cgctgttgaa gatgccggaa
    13381 gatttattga cccgttcggt aaacgttggt ttttccggcg gcgagaaaaa gcgcaacgat
    13441 attttgcaaa tggcggtgct ggaaccgggg ttatgcattc ttgatgaatc ggactccggg
    13501 ctggatattg acgcattaaa agtggttgcc gatggcgtga actcgctgcg tgacggcaag
    13561 cgctcattca tcattgttac gcactaccaa cgcattctcg actacatcaa gcctgattac
    13621 gtccatgtgc tatatcaggg acgaattgtg aaatccggcg atttcacgtt ggtcaaacaa
    13681 ctggaggagc agggttatgg ctggcttacc gaacagcagt aacgcactgc aacagtggca
    13741 tcatttgttt gaagctgaag gggtgaaacg ttccccgcaa gcacaacagc atttagaaca
    13801 attgctgcgt accggactgc cgacacgtaa acatgaaaac tggaaatata cgcctctgga
    13861 agggctgacc aatagccagt ttgtcagcat tgcgggggag atatccccac agcagcgtga
    13921 tgccttagcg ttaacgttag acgccgtgcg gctggtgttt gtcgatggac gttacgtgcc
    13981 tgcgctgagc gatgcgactg aaggcagcgg gtatgaagtg agcattaacg acgaccgtca
    14041 ggggttaccc gatgctattc aggcggaagt gtttctgcat ttgacggaaa gcctggcaca
    14101 aagcgtgacg catatcgccg tgaagcgcgg tcaacggccg gcaaagccat tgctgttaat
    14161 gcatatcacc cagggcgtgg caggtgaaga ggtgaacact gcccattacc gacatcatct
    14221 ggaactggcg gaaggtgccg aagcaacggt gatcgaacat tttgtcagcc ttaatgatgc
    14281 tcgtcatttt accggggcac ggttcactat caacgtcgca gcgaatgccc acttgcagca
    14341 tatcaagctg gcgtttgaaa acccgctcag tcaccacttt gcccataacg atttgttgct
    14401 ggctgacgat gccaccgcat ttagccacag tttcctgctg ggtggcgcag tgttacgaca
    14461 caacaccagt acgcaactca atggcgaaaa cagcatgttg cggatcaata gcctggcgat
    14521 gccggtcaaa aacgaggtgt gtgatacccg tacctggctg gaacacaata aaggtttttg
    14581 taacagccga cagttgcaca aaactatcgt cagcgacaaa ggccgcgcgg tatttaacgg
    14641 tttgatcaac gtcgcgcagc acgccatcaa aacggatggt cagatgacca acaataatct
    14701 gctgatgggc aaactggcgg aagtggatac gaaaccgcag ctggaaatct atgcagatga
    14761 tgtgaaatgc agccacggcg cgacggtggg gcgtattgat gatgaacaga tgttctatct
    14821 gcgctcgcgc gggatcaatc agcaggatgc ccagcagatg atcatttacg cctttgctgc
    14881 cgaactgacg gaagcactgc gtgatgaggg gcttaaacag caggtgctgg cccgaatcgg
    14941 tcaacggctg ccaggaggtg caagatgact ttttccgtcg acaaagtgcg ggccgacttt
    15001 ccggtgcttt cgcgtgaggt aaacggtttg ccgctggctt atctcgacag cgccgccagt
    15061 gcgcagaaac cgagccaggt gattgacgcc gaggccgagt tttatcgtca tggctacgcg
    15121 gcggtgcatc gcggtattca taccttaagc gcccaggcga ccgagaaaat ggagaacgtg
    15181 cgcaagcggg catcgctgtt tattaatgcc cgttcggcgg aagagctggt gttcgtccgc
    15241 ggcacgacgg aagggatcaa tctggtcgcc aatagctggg gaaacagcaa cgtgcgggcg
    15301 ggcgataaca tcatcataag tcagatggag caccacgcta acattgttcc atggcagatg
    15361 ctttgcgcac gcgttggcgc agagctgcgt gtgatcccgc tcaatcccga cggtacgttg
    15421 caactggaga tgctacctaa tctgttcgat gagaaaactc gcctgctggc aattactcat
    15481 gtctccaacg tgcttggcac agaaaatcca ctggcggaaa tgatcacgct tgcgcaccag
    15541 catggcgcaa aagtgctggt ggatggcgct caggcggtga tgcatcatcc ggaggatgtt
    15601 caggcgctgg attgcgactt ttacgtgttc tccgggcata aactgtatgg ccccaccgga
    15661 attggcattc tttatgtgaa agaagccttg ttgcaggaga tgccgccgtg ggaagggggc
    15721 ggttctatga tcgccaccgt cagcctgagt gaaggcacta cctggaccaa agcaccatgg
    15781 cggtttgaag ccggtacacc caataccggg ggcatcattg gtcttggcgc ggcgctggag
    15841 tatgtttcgg cgctggggct taataacata gccgagtatg aacagaatct gatgcactac
    15901 gcgctatcac agttggaatc tgtaccggat ctcactctct atggcccaca aaacaggctt
    15961 ggcgttattg cttttaatct cggtaaacac cacgcctatg atgttggcag ttttctcgat
    16021 aattacggca ttgctgtgcg taccggacat cactgcgcta tgccattaat ggcctattac
    16081 aacgtccctg cgatgtgtcg ggcgtcgctg gccatgtata acacccatga agaagtggat
    16141 cgtctggtga ccggattgca acgtattcac cgtctgctgg gataacaggg aggcactatg
    16201 gctgtattgc cggataaaga aaaattgctg cgtaattttt tacgctgcgc caactgggaa
    16261 gagaaatatc tctacattat tgagctgggc cagcgtctgc cagaattacg cgacgaagac
    16321 agaagcccac aaaatagcat tcagggctgc cagagtcagg tgtggattgt catgcgccag
    16381 aatgcgcagg gaattattga attgcagggc gacagcgatg cggcgattgt gaaagggctt
    16441 attgcggtcg tctttattct ctacgatcag atgacgccgc aggatattgt caatttcgat
    16501 gtgcgtccgt ggtttgaaaa aatggcgctc acccaacatc tcaccccatc tcgttcacaa
    16561 ggtctggaag cgatgattcg cgcaattcgc gccaaagccg ctgcacttag ctaaactaaa
    16621 gggacagctt tcatccggtt ggctcacatt gccggatggt caatgtttac actgacctgc
    16681 acaggagtgt ttcggtttca ggtgaacata agcgccatcc gacgtttcag cgtgagtctc
    16741 cggcaaatac aggaggttta caatgaaacg cgcgtctttg cttacactca cgcttatcgg
    16801 cgcttttagc gccatccagg ctgcctgggc ggttgattat ccgctaccac caaccggaag
    16861 ccgactggtt gggcaaaatc aaacgtatac ggtgcaggaa ggggataaaa accttcaggc
    16921 catcgcccga cgttttgata ctgcggcaat gttgatcctt gaagccaata acactatcgc
    16981 cccggtgcca aaacccggta cgacgataac tattccttcg caactgttat tacctgatgc
    17041 gccgcgtcag gggattatcg ttaaccttgc agagctgcgc ctttattatt atccgccggg
    17101 agaaaatatc gtgcaggttt atccgatagg tattggcttg caggggctgg aaacgccggt
    17161 gatggaaacg cgtgtggggc agaaaatccc taacccaacc tggacgccta cggcaggcat
    17221 tcgtcaacgt tcgctggagc gtggcattaa attaccgcca gtcgttcctg ccggaccaaa
    17281 taacccgcta ggacgttacg cactgcgact cgcgcatggt aatggcgaat acctcattca
    17341 tggcaccagt gcgccggaca gcgtcggttt gcgcgtcagt tcagggtgta ttcgcatgaa
    17401 tgcaccggat attaaagcct tgttctccag cgtgcggacg ggaacgccgg tgaaagtgat
    17461 caacgaaccg gtgaaatatt ccgtggagcc taacgggatg cgttatgttg aagtacatcg
    17521 accactatcg gcagaagaac agcagaacgt tcagacaatg ccatacacac tgccagcagg
    17581 ctttacgcaa tttaaagaca ataaggctgt agatcagaag ttagtcgata aagcgttgta
    17641 tcgtcgggca gggtatccgg ttgcggtgag cagtggagca actcccacag ccagcaatgc
    17701 gccttcagta gagtcagcgc agaatggtga accagagcaa gggaatatgt tacgcgcgac
    17761 gcagtagtgg taaatagcag acaaaaaaaa tggcgcacaa tgtgcgccat ttttcacttc
    17821 acaggtacta ttacttgcgg tatttagtag ccatgttgtc cagacgctgg ttagcacgag
    17881 ctgcgtcatc tttagcagcc tgaacgtcgg aacgcattgc gttcacgtcg ttgctcagct
    17941 ggtcaacttt agcgttcaga gtctgaacgt cagaagacag ctgatcgatt ttagcgttgc
    18001 tggagcaacc tgccagcaga gtagaaccca ggattaccgc gcccagtacc agtttagtag
    18061 ctttcattat taataccctc tagattgagt taatctccat gtagcgttac aagtattaca
    18121 caaagttttt tatgttgaga atattttttt gatgggaagg cacttatttt tgatcgttcg
    18181 ctcaaagaag catcgaacgc tgggttttag ataaaaaaat tgagagaaaa cagga
//
LOCUS       DAZKFU020000082        18178 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZKFU020000082 DAZKFU020000000
VERSION     DAZKFU020000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.082"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2261
                     /locus_tag="ACHOQQ_RS11120"
                     /old_locus_tag="pgaptmp_002224"
     CDS             <1..2261
                     /locus_tag="ACHOQQ_RS11120"
                     /old_locus_tag="pgaptmp_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HGU4470465.1"
                     /translation="VICMVLFFMTGYACATEWISSEDLITSDFHLMTADERNVVKAAT
                     DDSMEAAYMLKDNIRWYYHNGNLSLPANFSNQNKLVVNGNLTISGDYDDYLSGNGHLI
                     VLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVSDKATQFE
                     VIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPDYDSVADN
                     IVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTESLSPAVM
                     LQLLQHPDDQTRESMAQSWPAQQMHLLTDELIKDEAVARGLVKNSNISADVNKKLMSV
                     PVESVQLEQARQDNLSPDIVASLSHSPFLNVRKTLLSHYDYAWLVPTAVADELINSED
                     PELRERITGADLTAQQAVLLSKDKSLKVREALARTLTELKITQLSATLRTEDIERIAE
                     QMYLDNKENKNIVKALLIALPEMRQLSLAKEDVHNLREGTRYLTSKEVISYLLTQHDV
                     PTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNGVVDEEML
                     NNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVVSMKNSTR
                     RQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASL
                     LTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSLLRDLVKTGATRKI
                     RSEARHRLEEKQ"
     gene            2258..2713
                     /locus_tag="ACHOQQ_RS11125"
                     /old_locus_tag="pgaptmp_002225"
     CDS             2258..2713
                     /locus_tag="ACHOQQ_RS11125"
                     /old_locus_tag="pgaptmp_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470466.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(2791..3702)
                     /gene="rdgC"
                     /locus_tag="ACHOQQ_RS11130"
                     /old_locus_tag="pgaptmp_002226"
     CDS             complement(2791..3702)
                     /gene="rdgC"
                     /locus_tag="ACHOQQ_RS11130"
                     /old_locus_tag="pgaptmp_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU4470467.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            3827..4735
                     /gene="mak"
                     /locus_tag="ACHOQQ_RS11135"
                     /old_locus_tag="pgaptmp_002227"
     CDS             3827..4735
                     /gene="mak"
                     /locus_tag="ACHOQQ_RS11135"
                     /old_locus_tag="pgaptmp_002227"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGU4470468.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(4869..6053)
                     /gene="araJ"
                     /locus_tag="ACHOQQ_RS11140"
                     /old_locus_tag="pgaptmp_002228"
     CDS             complement(4869..6053)
                     /gene="araJ"
                     /locus_tag="ACHOQQ_RS11140"
                     /old_locus_tag="pgaptmp_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGU4470469.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(6179..9322)
                     /gene="sbcC"
                     /locus_tag="ACHOQQ_RS11145"
                     /old_locus_tag="pgaptmp_002229"
     CDS             complement(6179..9322)
                     /gene="sbcC"
                     /locus_tag="ACHOQQ_RS11145"
                     /old_locus_tag="pgaptmp_002229"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGU4470470.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTVPQEDEEESWLATRQQEAQSWQQHQN
                     ELTALQNRIQQLTPILETLPQSDELPHCEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALVDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPGGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(9319..10521)
                     /gene="sbcD"
                     /locus_tag="ACHOQQ_RS11150"
                     /old_locus_tag="pgaptmp_002230"
     CDS             complement(9319..10521)
                     /gene="sbcD"
                     /locus_tag="ACHOQQ_RS11150"
                     /old_locus_tag="pgaptmp_002230"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGU4470471.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            10711..11400
                     /gene="phoB"
                     /locus_tag="ACHOQQ_RS11155"
                     /old_locus_tag="pgaptmp_002231"
     CDS             10711..11400
                     /gene="phoB"
                     /locus_tag="ACHOQQ_RS11155"
                     /old_locus_tag="pgaptmp_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGU4470472.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            11458..12753
                     /gene="phoR"
                     /locus_tag="ACHOQQ_RS11160"
                     /old_locus_tag="pgaptmp_002232"
     CDS             11458..12753
                     /gene="phoR"
                     /locus_tag="ACHOQQ_RS11160"
                     /old_locus_tag="pgaptmp_002232"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGU4470473.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            13160..14479
                     /gene="brnQ"
                     /locus_tag="ACHOQQ_RS11165"
                     /old_locus_tag="pgaptmp_002233"
     CDS             13160..14479
                     /gene="brnQ"
                     /locus_tag="ACHOQQ_RS11165"
                     /old_locus_tag="pgaptmp_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGU4470474.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            14555..15928
                     /gene="proY"
                     /locus_tag="ACHOQQ_RS11170"
                     /old_locus_tag="pgaptmp_002234"
     CDS             14555..15928
                     /gene="proY"
                     /locus_tag="ACHOQQ_RS11170"
                     /old_locus_tag="pgaptmp_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGU4470475.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            16084..17901
                     /gene="malZ"
                     /locus_tag="ACHOQQ_RS11175"
                     /old_locus_tag="pgaptmp_002235"
     CDS             16084..17901
                     /gene="malZ"
                     /locus_tag="ACHOQQ_RS11175"
                     /old_locus_tag="pgaptmp_002235"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGU4470476.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGSQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            18089..>18178
                     /locus_tag="ACHOQQ_RS11180"
                     /old_locus_tag="pgaptmp_002236"
     CDS             18089..>18178
                     /locus_tag="ACHOQQ_RS11180"
                     /old_locus_tag="pgaptmp_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470477.1"
                     /translation="MTISNSNLLKPFITGAELPPFKEDVFSKTE"
BASE COUNT         4214 a         4474 c         4836 g         4654 t
ORIGIN      
        1 tggtcatctg tatggtctta ttttttatga ctggctatgc atgtgccaca gagtggatca
       61 gtagcgagga ccttatcacc tctgactttc atcttatgac ggcagatgag cgcaacgtgg
      121 ttaaagcggc aaccgacgat agtatggaag ctgcttatat gctaaaagat aatatccgct
      181 ggtattatca taatggtaac ctatcattac ccgctaactt cagcaatcaa aataaactgg
      241 tggtgaacgg taatttaacg atttccggcg attatgatga ttatctgtca ggtaatggtc
      301 atctgattgt attaggtaat gttattgtcg ataactttat taatcatgat ttcgcgtatg
      361 tcaaaggaca gatgacagcg aaaggtttag tttatgctga ttacaatgat cataactttg
      421 aagtgatgaa gggaattagt gcgcgaggca ttattgtttc cgataaagca acgcaatttg
      481 aagtcattaa agctgaattt tatatcaatg aagatggaag tggtgaagga tataactggg
      541 atgagaatat tcaaaaagcc tattcactgg tgactgccga tctttatgat cacactgaaa
      601 tcgaaaccga taacatttcc aatgcgtatc ctgactatga tagcgtcgca gacaacatcg
      661 ttcagggttt accgcttttt cgagataaag ccgctcctga aattaatgag aaattaaaat
      721 ggattgaaac ggggaaactc gataatttcc cagcgaataa aataaagcat caagatccac
      781 tggtggcacg ttttctgact catacagaga gtttatcacc cgccgttatg ctgcaactgt
      841 tacagcaccc cgatgaccag acgcgtgaat cgatggcgca gtcctggccc gctcaacaga
      901 tgcatttgct taccgatgaa ttaattaaag atgaggctgt tgccagaggg ctggttaaaa
      961 acagtaatat ttccgccgat gtgaataaaa aattaatgtc tgtacccgtc gagagcgtac
     1021 agctggagca ggccagacag gataatcttt ctccagatat tgttgcgtcg ttaagtcata
     1081 gcccctttct caatgtacgc aagacattac tcagccatta cgattacgcc tggctggttc
     1141 cgacagcagt ggccgatgag ttgataaata gcgaagatcc ggagttacgc gaaagaatta
     1201 ccggagcaga tttaaccgca caacaagccg tgctgttgag taaagataaa tcactgaaag
     1261 ttcgtgaagc attagcccgg acgcttactg aattaaaaat tacccaactg tcagcaaccc
     1321 tgcgtacaga ggatattgaa cgtatcgcag aacaaatgta tctggataat aaagaaaata
     1381 aaaatatcgt aaaagcgtta ttgatagcgt tgccggagat gcgtcaactg tctctggcaa
     1441 aagaagatgt gcacaattta cgcgaaggta cgcgttatct gacatcgaag gaagtgatta
     1501 gttatctact gactcagcat gatgttccca ccgtctggga tgagctggca cgcgataaat
     1561 tattgccgct ggaatataag aaacagttat ggcaacgcac attaaattta atgatgagca
     1621 aacggcaaga agatcaagag caagcctacg aagtacagct ggcgctgatt gataatggtg
     1681 ttgttgacga agagatgtta aataatgcca ttgatttact ggttgattta cccgctgaat
     1741 accgctatcg aatgcgtaac caattatttg ataataaaga attaccttcc ggaattatca
     1801 acaaactcga tcaacagtat cgcttcaact ctgactgggc gctggctgtt gtcagtatga
     1861 aaaatagcac ccgtagacaa agtgaaagag gattacaccg ctggaaccat gaggatagcg
     1921 atatttttgc ggaactggcg acgataaaag ataaatcaga tgatgaatgg tggcgtgcat
     1981 tgctgcaaag tcgcaatgat catttgcgcc aaacggcatt acgcaacgca cataccccgg
     2041 cgtcgttgtt aacgacattg actgagcctc aggatcggtc gctggctatt aataatccac
     2101 agctggctgc cgatgtgaaa acggcgtggt taaaagagga tccatcatta ctcttatttg
     2161 tcgaacaacc cgatctttcg ctgttacgtg atttagtgaa aaccggggca acgcggaaaa
     2221 ttcgcagtga agcgcgtcac cggcttgagg aaaaacaatg acacagcgcc catggagcaa
     2281 gttacagcgg gagatctatg acttactgac gccaacgatc aacctgcaaa ttcactgtac
     2341 ccgttatccc atgcgcagcc aaaatggtgg aagcaccgat ttaccgcgtt attggatcac
     2401 gttggataaa aacgtgatct gggattaccc gaaagacttt attgcaggta acggtggagt
     2461 gcgtaatttt cacggtgaaa cttgctggta tccttacctg acggatattt gttcaatttc
     2521 tgatttattg cgtgaatata ttgacacccc aaaagcggag ctgttaacaa aacaatttac
     2581 cagcgataaa tgggggttag tgaatattct gcgtgctgct gacaggcgaa tagggatgag
     2641 gcgattagat cagttacggc gtaaaacgca taatattgct gcgctaaaaa ttattgcccg
     2701 ccgtagcgaa taattatatg cctggtgtgg cttcgtacgc cggataagac gcggcaggcg
     2761 tcgcatccgg cattaaaggt aaatcagcaa ttaacgttgt gcttcgccac ctaatccttc
     2821 aatcaggttt tgaattaacg ctgccagttc accagtcatc aggatgaaat cggcatcaaa
     2881 acgctgggcg aaatcttcac ggtcgatatc ttcgttttga tcgcgcagct cgtcgcagaa
     2941 cttcagacgc ttgagcgaac catcgtcgca catcacaaac tgaatgcgct gctgccagtc
     3001 gagcgccagt ttagtcacca cttttccggc ttcaatgtga ttggtgatct cttcgctggt
     3061 cagatcttgt ttcttcgcgc ggatcacgcc gccatcttcc agcaacgatt tcagctcggc
     3121 ttcatcaagc agctggaagc cctgtgccgc actaccggaa cgtacccatt cggtcagcgt
     3181 cagttcaatc gggttttcca tgctcaatgg tacaaccggt aacgacccca ggcttttacg
     3241 cagcagtgcc agcgtatctt ccgctttttt ggcactggcg cagtccacca taatcaaacc
     3301 gttaaccgtg tcgatccaca tcattgtctg gctaaaacgg ctgaaagcac gcggcaacag
     3361 agagtgcagc acttcgtctt tcagcgaatc tttttcggtt ttcttcagct tacgcgcctg
     3421 ttccgcttcc agtttggcga ttttcgcttc cagcgcctgt ttaatcaccg gagacgggag
     3481 gattttttct tctttgcgtg cgcagataac aatttgacca ttggcaacgt gcgttaacgc
     3541 atcgctgtgc gatcccatcg gaggaaccca gcccatcttc gccatgtcct ggctgccgca
     3601 tggggtaaat gccatcgagg ctagctgttt ttccatctct tctgcacgca gcgaaatctc
     3661 gcggctaaga cggtaaacca ttaaattctt gaaccacagc atgataattt ccacggcctt
     3721 gtcgttaaat ttagcgggca tgataacgaa ttgtcggcgg ccttgcattg ccaatccggt
     3781 tgtccgtctc tacgctattg atattgaaaa aaataaggag agtaccgtgc gtataggtat
     3841 cgatttaggc ggcaccaaaa ctgaagtgat tgcactgggc gatgcagggg agcagttgta
     3901 ccgccatcgt ctgcccacgc cgcgtgatga ttaccggcag actattgaaa cgatcgccac
     3961 gttggttgat atggcggagc aggcgacggg gcagcgcgga acggtaggta tgggcattcc
     4021 tggctcaatt tcgccttaca ccggtgtggt gaagaatgcc aattcaacct ggctcaacgg
     4081 tcagccattc gataaagact taagcgcgag gttgcagcgg gaagtgcggc tggcaaatga
     4141 cgccaactgt ctggcggttt cagaagcagt agatggcgcg gcagcgggag cgcagacggt
     4201 atttgccgtg attatcggca cgggatgcgg cgcgggcgtg gcattcaatg ggcgggcgca
     4261 tatcggcggc aatggcacgg caggtgagtg gggacacaat ccgctaccgt ggatggacga
     4321 agacgaactg cgttatcgcg aggaagtccc ttgttattgc ggtaaacaag gttgtattga
     4381 aacctttatt tcgggcacgg gattcgcgac ggattatcgt cgtttgagcg gacatgcgct
     4441 gaaaggcagt gaaattatcc gcctggttga agaaagcgat ccggtagcgg aactggcatt
     4501 gcgtcgctac gagctgcggc tggcaaaatc gctggcacat gtcgtgaata ttctcgatcc
     4561 ggatgtgatt gtcctggggg gcgggatgag caatgtagac cgtttatatc aaacggttcc
     4621 gcagttgatt aaacaatttg tcttcggcgg cgaatgtgaa acgccggtgc gtaaggcgaa
     4681 gcatggtgat tccagcggcg tgcgcggcgc tgcgtggtta tggccacaag agtaaaaaac
     4741 ccaggcaatt ggcgcatcat gcctgatgcg acgcttgccg cgtcttatca ggcctacaaa
     4801 atgtgccaga accgcgtaag gcgttcacgc cgcatccggc aataagcaac gtgctccgtg
     4861 actgcaatct accccagagg tttcgccagc accggagtat ccgccgcttg ctggcgctta
     4921 tagcgaccat acagcagcaa tgacgacatc gcagcaaacg aaagcagggc ggcaggcagc
     4981 gccacgtaat tatatgccag ccccagcgtc agcatcatac caccgcaata tgcgccgacg
     5041 gcgctaccga ggttaaaggc tatttgccca cctgcggcac ctaataactc tccgcctttg
     5101 gcgttttgca gtaacaatat ttgcagaggt gctgaaaggg caaataatcc cgcgcaacaa
     5161 ataaaagcaa aaataagcga cgttgttttc ataccgccgc agaaaaagag catcagcagt
     5221 gccagtacaa ttataaagtc agtcactgct gcaatgcgca gtggtgaata acgtcctgaa
     5281 atcctgccac ttagcatatt tcccagcacc atccctagcc caactaacat cataataaag
     5341 gtcatcgccg tttccgaaaa accggaaata aacatcatgt atggctttac gtagctgaac
     5401 caggcaaaca cacctgcgtt gccaaacatg gtggcggcga aaattaacca cggggccggg
     5461 ctgcgcaaaa agtgaaattg ttcgcgcagc tttcctttcg cctcgtcgcg aatatccggc
     5521 acccaaaaat agaccgatgc catcaccgca atattaaaaa cagcgatcaa taaaaaggtg
     5581 taacgccagc taaattcctg acttaaatac gttcccagcg gaatgcccag caaattggcg
     5641 actgtcatcc cggaaaccat ccccgccacg gcggcggtga cttttccggg tttgataatt
     5701 tttgataaca cgatcgctcc gacgccaaaa aatgcgccat gcggaaagcc ggaaaccagg
     5761 cgaccaatgg caagcatcag gtaagacgaa gagagcgtga acatggcgtt gccaatgacg
     5821 cacaacgcca ccagaaacaa caagatatgt ttgagtgagt agcggctgga aaagagtgcg
     5881 attattggcg caccgaccac cacccccagc gcataatacg agatcatatg cccggcagca
     5941 ggaatcgaaa ttcctacgtt atgcgccagc tccgtgagca cgcccataat gccaaattcg
     6001 gccatcccca aaccaaacgt gcccagagcc aaagataaaa tgactttttt cataccacca
     6061 tcacaaccac cagaacggct accaatcacc gtagccaata aagtgatagc ttgcaggata
     6121 acgaccagca aggccaagag ataattcgcc cctctgtatt cattatcctg ctgaatagtt
     6181 atttcactgc aaacgtactt tccagtttgc tgtagcccag tccattgatc tttttcactt
     6241 tgatctgcac cggaatacgc tctttcatcg cttctacgtg gctaatcaca ccgatggttt
     6301 tgccactggc gttcagggca tccagcgcat caagggcggt atccagcgtt tcgctatcca
     6361 gcgtgccaaa accttcatca aggaacagcg agtcaatacg tgttttatgg ctgaccagat
     6421 ctgaaagcgc cagtgccagc gccagactga cgaggaaact ttcgccgccg gaaagggtac
     6481 gggtatcgcg taccgcatct gcctgccagg tatcaacaac ctcgacttcc agcgcctcgc
     6541 tggctttgcg ctgtaacaga tagcgcccgt gcagccgggt aagttgctga ttagcgagat
     6601 ggactaaatt atccagcgtc agcccctggg caaatttgcg gaatttatcg ccctctttgg
     6661 aacctattag cgaattcaga tatccccagt cctcaacctg ctgcgtcatt tgagcaattt
     6721 gctgcattaa ggtttgttgt tgctgacggt tatctgcatc ctgcttcagc tgctggcgaa
     6781 tctcgccttg actcgtggtg ttctcacgca atttttggtg agtttgcgct aactcttgct
     6841 gaatctgctc caccgtcaca gtgagagcca acccgccagg tcggtgttgc tgatgctgtg
     6901 ccagcgtttc tgctgtctga gtgaccagcg tttgcgcctg acggcgctgg ttttccagat
     6961 tctgcttgag ctgttccagc tgcgttagtg tttgttcatc cactagcgcc gcaaggaacg
     7021 cctgctgatc gtcaaagacg ctggcctgta gcgcggtgtc aaactgcgcc tgggcttttt
     7081 gcagactttg cgccgccaga acatcctgtt gctgtaacgt ctgctgctgg ctgtgtaatg
     7141 cgagacattg ttcatgtacc tgccgccagt tttccaatac cacagtttct tcgcagtgcg
     7201 ggagttcatc actttgcggc aacgtttcca gaatcggcgt cagctgctga atacggtttt
     7261 gcagcgcggt taattcgttc tggtgttgct gccagctctg cgcttcttgc tgacgtgtcg
     7321 ccaaccagct ctcttcttca tcttcctgtg gcactgtcag tgcataaccc gccaatgccg
     7381 ttaagagctg ctgctggcgt tgttcaattt gctgttgata ctggataatt tgctgattat
     7441 gcgcggcaat ctgcccttgt aattcatgtc gttggctgag taaccgcagc tggcgttcgt
     7501 gctcatcttg tgcatccagc cacggttgaa tatcgtcctg tggctgcaag gtgatattga
     7561 ggctggtcgt gacggcttgc cattgttgag taagtgcttg ctcatcttgt cggaggcttt
     7621 gcgcttcgtt ttcatcacgc tgaagctgct ttgttaaggc gtccagttgc ccacgtagcg
     7681 tcgcaccttc ttcaccgagc tttttaactt cgttttccag cgccagtaat cgagactgat
     7741 taacaccagg ctccagcgcc tgatacgcct cgaccgccgg gtggctggtg gaaccacaaa
     7801 gtgggcaagg ctgacccgcc tgtaactgtg cacgttgagc ttccagcgtt ttgatgcgcg
     7861 cttcctgctc gcaaatggtt ttgacatcgg caagttgctg cgtcttttct ttataacgct
     7921 ggcgcatttc gttaagtgcg gcattacgtt gcgtctgttc cagagtgaca ttctggatag
     7981 tgaccattaa ctgcgccaga cgtttttgtt ggggaacaat ctgcccatgc agcgcgacca
     8041 ggcgctgacg cagtgggcgt tgctcggcat gttgcgccag ggcgctagca acttcatcgg
     8101 cggttaacgt caacgtgatc gccgcaagcg tattaagttt ttgctcagca tgggttaact
     8161 gttgctgcca ttgccgcaga tgctcgcgat cgctggtttg ttgggagaac tgcgcacgcc
     8221 aacccgccag ttcgttgttc cactgacgga agcggtcgtg ttcctgtaac caggtattca
     8281 ggctttgttg ctgctgctgt aattctgctg actgcttcgc cgcgtggtgg cgaatgctcg
     8341 cgcgaagcgc cattgtgctc tgtaagcgag tatttacttc ttcaatctgc tggcgagtat
     8401 gcgccagcgc cgcgctgtgt tctgcgatgc gttcccagtg cggacgaaga tttcgtgccg
     8461 gttgtgccag actgagcgcc gccagttgag gttgcgcttt ttcttcttcg gctaacgcct
     8521 gttgcaaggc ctgttgacgg cggctggctt cttgctgcaa ttcgtccaga cgcgttaacc
     8581 agtttagtga ttgttgttct tgctgctgcg cggtaagtaa ctgtttttct tcgtcagtaa
     8641 gtacctgcaa actcgctgtc agcgattgca cttgttctgg cgtgagcaac gcgacgccgc
     8701 tggcctgcgc ttgcagcttc tccagctcag tgcgggccga tttgtgctgc tcaaaaacca
     8761 tcgccgagat ttgcccgtag atttcagtgc cagttaactc ctcgagcaat tccgcgcgtt
     8821 ctttgggttt ggcattcagg aaggcagcaa attgcccctg cgaaagcagc atcgaacggg
     8881 tgaagcgccc gtaatccagt ccggttaacg tcgccgtcag ttccagctta tctttcactt
     8941 tgtcggcgag aattttgcca tcggcgcagc gcgccagctc tacgcgtggc acctgcaaat
     9001 taccgtccgg ctggttacgc gcccgattct ggctccagaa cgcgcgatac gcttcacctt
     9061 tcacttcaaa ctccacctcc gccagacatt cggcggtatc gcgggtcatg agatcatttt
     9121 gcgattgtga aacgttagag aggcgtggag tttcgtgata cagcgccaga caaatggcgt
     9181 ccagcagggt ggttttcccc gcaccggttg ggccggtaat ggcaaacagc ccgttgctgg
     9241 cgaacggctc gcgggtgaaa tcaatcttcc attcgccttt taatgagttc aggtttttca
     9301 ggcgcaggct gagaattttc atgcttcgtg ttctccggcg agggtatgca acgtcgtggt
     9361 gaaaagatgc tgcagacgtt gctgctgcga ttcctccagt tcttccagtg ccagacggcg
     9421 attgaacacc tcttcgacgc tgagttcgct gagggtttca cgctgttggc tggctaacac
     9481 acgctcgcgc tgttcacgac tccggcgtac cagcaatact tcaacaggca atgattcggt
     9541 taatgcctgg attttgcgct gaatatcatg cagatactca tcagtagtga tttcgatatc
     9601 cagccagaca ggcggctcct gtgatacatc gcgccactgt tccagctgtg cggtaatcga
     9661 cgccagatcg cctttcagaa ccgccatggg ttgcgttacc ggtacgttca ggttttccac
     9721 gctctctaat ttgccgtttg aaaatgtcac cagatggaca tatttactct ttccgcattc
     9781 atcaaaactc agtggaatgg gggagccgca atagcgaaca tgttccatgc cgccaataat
     9841 ctgtgcgcgg tgaatatgcc cgagcgcgat gtagtcggct ggtggaaagt tttgtgccgg
     9901 aaacgcgtcc agcgtgccaa tataaatgtc acgcacggcg tcacttttac tggcccccac
     9961 ggtcgttaaa tgtcccgtgg cgatgatggg cagaggctga tcgccgcgca gtttgcaggc
    10021 atcggcatag tgttgttggt aataatcggt aatcgctgcc agtaaatgct gctgtttttc
    10081 aataccgtta agccccgcct ggctggtaat aatgtcacgt ggacgtaaaa acggaatggg
    10141 gcacagcacg gcgcctggcg tcccgtcgcg acgaggcaag atttgcggcg catgtccggc
    10201 gctggcgacc acggtcgtat tgaggaacgc catgatatcg cgcgattcat tcagcgtggc
    10261 gaccgagtca tggtttcctg ccagtaccac cagatgacag ccagtttgct gtaaattgac
    10321 aacaaaacgg ttgtataacg tgcgggcgta actgggcggc gagccggtat cgaaaacatc
    10381 accagcaaca ataatcgcat ccacctgatg ggtttgtgct gtttccagca gccagtcaag
    10441 aaaagcctga tgttcagctt cgcggctttt actgtagaag ttctggccga gatgccagtc
    10501 tgaggtgtga agaatgcgca taacggttcc ctggcgaaaa agcatgggcg cgattatacc
    10561 caaacagatg tgccatttgc ttttttctgc gccacggaaa tcaataacct gaagagatgt
    10621 gcgacgagct tttcataaat ctgtcataaa tctgacgcat aatgacgtcg cattaatgat
    10681 cgcaacctat ttattacaac agggcaaatc atggcgagac gtattctggt cgtagaagat
    10741 gaagctccaa ttcgcgaaat ggtctgcttc gtgctcgaac aaaatggctt tcagccggtc
    10801 gaagcggaag attatgacag tgctgtgaat caactgaatg aaccctggcc ggatttaatt
    10861 ctccttgact ggatgttacc tggcggctcc ggtatccagt tcatcaaaca cctcaagcgc
    10921 gagtcgatga cccgggatat tccagtggtg atgttgaccg ccagagggga agaagaagat
    10981 cgcgtgcgcg gccttgaaac cggcgcggat gactatatca ccaagccgtt ttcgccgaag
    11041 gagctggtgg cgcgaatcaa agcggtaatg cgccgtattt cgccaatggc ggtggaagag
    11101 gtgattgaga tgcagggatt aagtctcgac ccgacatctc accgggtgat ggcgggcgaa
    11161 gagccgctgg agatggggcc gacagaattt aaactgctgc acttttttat gacgcatcct
    11221 gaacgcgtgt acagccgcga gcagctgtta aaccacgtct ggggaactaa cgtgtatgtg
    11281 gaagaccgca cggtcgatgt ccacattcgt cgcctgcgta aagcactgga gcccggcggg
    11341 catgaccgca tggtgcagac cgtgcgcggt acaggatatc gtttttcaac ccgcttttaa
    11401 cgccttgctc atcggacgca gagcagggct tatgatttct taactggagt atcttacgtg
    11461 ctggaacggc tgtcgtggaa aaggctggtg ctggagctgc tactttgctg cctcccggct
    11521 ttcatcctgg gtgcattttt tggttacctg ccctggtttt tgctggcatc ggtaacggga
    11581 ctgcttatct ggcatttctg gaatttattg cgcctttcat ggtggctgtg ggtggatcgt
    11641 agtatgaccc cgccaccggg gcgtggtagc tgggaaccgc tactatacgg cttacaccag
    11701 atgcagctgc gaaataaaaa acgccgccgt gaactgggca atctgattaa acgctttcgt
    11761 agcggcgcgg agtcgctgcc cgacgcagtg gtgctgacca cggaagaggg cggtattttc
    11821 tggtgtaacg gtctggcgca acagattctt ggtttgcgct ggccggaaga taacgggcag
    11881 aacatcctta acctgctgcg ttacccggag tttacgcaat atctgaaaac gcgtgatttt
    11941 tctcgcccgc tcaatctggt gctcaacacc gggcggcatc tggaaattcg cgtcatgcct
    12001 tatacccaca aacagttgct gatggtggcg cgtgatgtca cgcaaatgca tcaactggaa
    12061 ggggcgcggc gcaacttttt cgccaacgta agccatgagt tacgcacgcc attgaccgtg
    12121 ttacagggtt acctggagat gatggatgag cagccgctgg aaggcgcggt acgcgaaaaa
    12181 gcgttgcaca ccatgcgcga gcagacccag cggatggaag ggttggtgaa gcaattgctg
    12241 acgctgtcga aaatagaagc cgcaccgacg catttgctca atgaaaaggt tgatgtgccg
    12301 atgatgctgc gcgttgttga gcgcgaggct cagactctga gtcagaaaaa acagacattt
    12361 acctttgaga tagataacgg cctcaaggtg tctggcaacg aagatcagct acgcagtgcg
    12421 atttcgaacc tggtctataa cgccgtgaat catacgccgg aaggcacgca tatcaccgta
    12481 cgctggcagc gagtgccgca cggtgccgaa tttagcgttg aagataacgg accgggcatt
    12541 gcaccggagc atattccgcg cctgaccgag cgtttttatc gcgttgataa agcgcgttcc
    12601 cggcaaaccg gcggtagcgg attagggtta gcgatcgtga aacatgcggt gaatcatcac
    12661 gaaagtcgcc tgaatattga gagtacagta ggaaaaggaa cacgtttcag ctttgttatc
    12721 ccggaacgtt taattgccaa aaacagcgat taatccgcct ttgtcatctt ttattgccat
    12781 aagccagccg atgttgtctt attttctttg cagtcaaaat acgggcgtta gattttacaa
    12841 cggttggtga ttttttgttc gcatgattag ccatgtcttt ttcacggaaa tagtgtttta
    12901 tactagttgg tgatttctta tcgctatata cctctggttt ttagatccct ccttgcttta
    12961 aaacgttata agcgtttaaa ttgcgcttca ggtgctgtca tactgactgc attaacgcgg
    13021 taaatcgaaa aactattctt cgccgcgcct ggttgggagt atttcccgct aaaattgttt
    13081 aaatataccg ctgtatcatc cccagggatt ggcacaaaaa tttaacgtta caacaccaca
    13141 tccacaggca gtatgattta tgacccatca attaagatcg cgcgatatca tcgctctggg
    13201 ctttatgaca tttgcgttgt tcgtcggcgc aggtaacatt attttccctc caatggtcgg
    13261 cttgcaggca ggcgaacacg tctggactgc ggcattcggc ttcctcatta ctgccgttgg
    13321 cctgccggta ttaacggtag tggcgctggc aaaagttggc ggcggtgttg acagcctcag
    13381 cacgccaatc ggtaaagtcg ctggcgtact gctggcaacg gtgtgttacc tggcggtggg
    13441 gccgcttttc gctacgccgc gtacagctac cgtttctttt gaagtgggga ttgcgccgct
    13501 gacgggtgat tccgcactgc cgctgtttat ctacagcctg gtctatttcg ctatcgttat
    13561 tctggtttcg ctctatccgg gcaagctgct ggataccgtg ggcaacttcc ttgcaccgct
    13621 gaaaattatc gcgctggtca tcctgtctgt tgccgcgatt gtctggccgg cgggttctat
    13681 cagcacggcg actgaggctt atcaaaacgc tgcgttttct aacggcttcg ttaacggcta
    13741 tctgaccatg gatacgctgg gcgcaatggt gtttggtatc gttattgtta acgcggcgcg
    13801 ttctcgtggc gttactgaag cgcgtctgct gacccgttat accgtctggg ctggcctgat
    13861 ggcgggtgtt ggtctgactc tgctgtacct ggcgctgttc cgtctgggtt cagacagcgc
    13921 gtcgctggtc gatcagtctg caaacggcgc tgctattctg catgcttacg ttcagcacac
    13981 ctttggcggc ggcggtagct tcctgctggc ggcgttaatc ttcatcgcct gcctggtaac
    14041 ggcagttggc ctgacctgtg cttgtgcaga attctttgcc cagtacgtac cgctctctta
    14101 tcgtacgctg gtgtttatcc tcggcggctt ctcgatggtg gtttctaacc tcggcttaag
    14161 ccagctgatc cagatctccg taccggtgct gaccgctatt tatccgccgt gtatcgcact
    14221 ggttgtatta agttttacac gctcatggtg gcataattcg tcccgcgtga ttgctccgcc
    14281 gatgtttatc agcctgcttt ttggtattct cgacgggatc aaagcatctg cattcagcga
    14341 tatcttaccg tcctgggcgc agcgtttacc gctggccgaa caaggtctgg cgtggttaat
    14401 gccaacagtg gtgatggtgg ttctggccat tatctgggat cgcgcggcag gtcgtcaggt
    14461 gacctccagc gctcactaaa tcactgaaca tttgttttaa ccacggggct gcgatgcccc
    14521 gtggtttttt attgtgttga tgggttagga attgatggaa agtaagaaca agctaaagcg
    14581 tgggctaagt acccgacaca tacgctttat ggcactgggt tcagcaattg gcaccgggct
    14641 gttttacggt tcggcagacg ccatcaaaat ggccggtccg agcgtgttgt tggcctatat
    14701 tatcggtggt atcgcggcgt atatcattat gcgtgcgctg ggggaaatgt cggtacataa
    14761 cccggccgcc agctctttct cgcgttatgc gcaggaaaac ctcggcccgc tggcaggtta
    14821 cattaccggt tggacctact gctttgaaat ccttattgtc gccatcgccg atgtgaccgc
    14881 ttttggtatc tatatgggtg tctggttccc gacggtgccg cactggattt gggtactgag
    14941 cgtggtgctg atcatttgcg ccgtaaacct gatgagcgtg aaggtattcg gtgagctgga
    15001 attctggttc tcgttcttta aagtcgccac catcatcatc atgattgtcg ccggtttcgg
    15061 catcatcatc tgggggattg gcaacggcgg gcaaccgacc ggtattcata acctgtggag
    15121 caacggcggc ttcttcagta acggctggct tggcatggta atgtcgttgc aaatggtgat
    15181 gtttgcttac ggtgggatcg aaattatcgg gattaccgcc ggtgaagcga aagatcctga
    15241 gaaatctatt ccgcgtgcga ttaactccgt gccgatgcgt attctggtgt tctatgttgg
    15301 tacactgttc gtcattatgt ctatctaccc gtggaatcag gttggcactg ccggtagccc
    15361 gttcgtgctg acgttccagc atatgggcat tacctttgcc gccagcattc ttaactttgt
    15421 tgtgctgact gcttcgctgt cggcaattaa cagtgacgta tttggcgtag gccgtatgct
    15481 ccacggtatg gcagagcagg gcagcgcacc gaaaattttc agcaaaacgt cgcgtcgcgg
    15541 tattccgtgg gttacggtgc tggtgatgac taccgcgctg ctgtttgcgg tgtatctgaa
    15601 ctacatcatg ccggaaaacg tcttcctggt gatcgcttcg ctggcaacct tcgccacggt
    15661 atgggtgtgg attatgatcc tgctgtcgca aatcgccttc cgtcgccgtt tgccgccaga
    15721 agaagttaag gcgctgaaat ttaaagtgcc gggtggggta gcaacgacca tcggcgggct
    15781 gattttcctg ctctttatta tcgggttgat tggttatcac ccggatacgc gtatctcgct
    15841 gtatgtcggt ttcgcgtgga ttgttgtgct gttgattggc tggatgttta aacgccgcca
    15901 cgatcgtcag ctggctgaaa accagtaata cttgcgttct gtaagccggt tcaggaggaa
    15961 atcctgatcc ggctattccg aaagttatcc gcccccgtcc tcctccccca aatatccttc
    16021 agatgatgag tgatcctgca ttaggctatg gcaaggtgat cagattttca tcacagggga
    16081 attatgatgt taaatgcatg gcacctgccg gtgcccccat ttgttaaaca aagcaaagat
    16141 caactgctca ttacactgtg gctgacgggc gaagacccac cgcagcgcat tatgctgcgt
    16201 acagaacacg ataacgaaga aatgtcagta ccgatgcata agcagcgcag tcagccgcag
    16261 ccaggcgtca cggcatggcg ggcggcgatc gatctctcca gcggacaacc ccggcggcgt
    16321 tatagtttca aattgctttg gcacgatcgc cagcgttggt ttacaccgca aggcttcagc
    16381 cgaatgccgc cagcacgact ggagcagttt gccatcgatg taccggatat cggttcacaa
    16441 tgggctgcgg atcagatttt ttatcagatc ttccctgatc gtttcgcccg tagtcttcct
    16501 cgtgaagctg aacaggatca tgtctattac catcacgcag ccggacaaga gatcatattg
    16561 cgtgactggg atgaaccggt cacggcgcag gcgggcggat caacgttcta tggcggcgat
    16621 ctggacggga taagcgaaaa actgccgtat ctgaaaaaac ttggcgtgac ggcgttgtat
    16681 ctcaatccgg tgtttaaagc tcccagcgta cataaatacg ataccgagga ttatcgccat
    16741 gtcgatccgc agtttggcgg tgatggggcg ttgctgcgtt tgcgacacaa tacgcagcag
    16801 ctgggaatgc gtctggtgct ggacggcgtg tttaaccaca gtggcgattc ccatgcctgg
    16861 tttgacaggc acaatcgtgg cacgggggga gcttgtcaca accccgaatc gccttggcgc
    16921 gactggtact cgtttagtga tgatggcacg gcgctcgact ggcttggcta tgccagcttg
    16981 ccgaagctgg attatcagtc ggaaagtctg gtgaatgaaa tttatcgcgg ggaagacagt
    17041 attgtccgcc actggctgaa agcgccgtgg aatatggacg gctggcggct ggatgtggtg
    17101 catatgctgg gggaagcggg tggggcgcgc aataatttgc agcacgttgc tgggatcacc
    17161 gaagcggcga aagaaaccca gccggaagcg tatatagttg gagagcattt tggcgatgca
    17221 cggcaatggt tacaggccga tgtggaagat gccgccatga actatcgcgg cttcacattc
    17281 ccgttgtggg gatttcttgc caataccgac atctcttacg atccgcagca aattgacgcc
    17341 caaacctgta tggcctggat ggataattac cgcgcagggc tttctcatca acagcaatta
    17401 cgtatgttta atcagctcga cagccacgat actgcgcgat ttaaaacgct gctcggtcgg
    17461 gatgttgcgc gcctgcccct ggcggtggtc tggctgttca cctggcctgg tgtaccgtgc
    17521 atttattacg gtgatgaagt gggactggat ggtaaaaacg atccgttttg ccgtaaaccg
    17581 ttcccctggc aggtggaaaa gcaggatacg gcgttattcg cgctgtacca gcgaatgatt
    17641 gtgctgcgta agaaaagtca ggcgctgcgt cgtggcggct gtcaggtact gtatgcggaa
    17701 gataacgtgg tggtatttgt ccgcgtgcta aatcagcagc gtgtactggt ggcaatcaac
    17761 cgtggcgagg cctgtgaagt ggtgctcccc gcgtcaccgt ttcttaatgt cgcgcaatgg
    17821 caacgcaaag aaggccatgg gcaactgaca gacgggattc tggctttgcc tgccatttcg
    17881 gctacggtgt ggatgaacta aagcatctgt tttatttgtt acgtcgttag tcaccagccc
    17941 tgacactacg cagcgcaaaa aagcggctca atgtcagggc tgaatttgtt aaatatttgt
    18001 cccgatcgat atgagcgatt tttggtattc tttgcccatg atgttatgta caacatgctc
    18061 caggataacc ctgtagtgga gcaaagatat gacaatttca aattcgaatt tattgaaacc
    18121 gtttattact ggtgctgagt tgccaccttt taaggaggat gtcttttcta aaacagaa
//
LOCUS       DAZKFU020000083        18006 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZKFU020000083 DAZKFU020000000
VERSION     DAZKFU020000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.083"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..997
                     /locus_tag="ACHOQQ_RS11185"
                     /old_locus_tag="pgaptmp_002237"
     CDS             <1..997
                     /locus_tag="ACHOQQ_RS11185"
                     /old_locus_tag="pgaptmp_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HGU4470478.1"
                     /translation="VQSLRELYTDVIEEFYWVALPLTTQNSLSQYQPEWQCWEPDVEW
                     VRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPAAMMIGIRADESYNRFVA
                     IASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTWYANHQSLCNPLYNLMYQ
                     AGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVSGVKSGGIYAGHDNHFYG
                     HRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHWYQKKGIEVPQTQQGDIG
                     AKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERIKGKRQEWGILCNND"
     gene            970..1599
                     /locus_tag="ACHOQQ_RS11190"
                     /old_locus_tag="pgaptmp_002238"
     CDS             970..1599
                     /locus_tag="ACHOQQ_RS11190"
                     /old_locus_tag="pgaptmp_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU4470479.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1600..2760)
                     /gene="ybdL"
                     /locus_tag="ACHOQQ_RS11195"
                     /old_locus_tag="pgaptmp_002239"
     CDS             complement(1600..2760)
                     /gene="ybdL"
                     /locus_tag="ACHOQQ_RS11195"
                     /old_locus_tag="pgaptmp_002239"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGU4470480.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            2869..3957
                     /gene="hcxA"
                     /locus_tag="ACHOQQ_RS11200"
                     /old_locus_tag="pgaptmp_002240"
     CDS             2869..3957
                     /gene="hcxA"
                     /locus_tag="ACHOQQ_RS11200"
                     /old_locus_tag="pgaptmp_002240"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGU4470481.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNNAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3967..4164)
                     /locus_tag="ACHOQQ_RS11205"
                     /old_locus_tag="pgaptmp_002241"
     CDS             complement(3967..4164)
                     /locus_tag="ACHOQQ_RS11205"
                     /old_locus_tag="pgaptmp_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4470482.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4346..6451)
                     /gene="cstA"
                     /locus_tag="ACHOQQ_RS11210"
                     /old_locus_tag="pgaptmp_002242"
     CDS             complement(4346..6451)
                     /gene="cstA"
                     /locus_tag="ACHOQQ_RS11210"
                     /old_locus_tag="pgaptmp_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGU4470483.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKEMP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6632..7045)
                     /gene="entH"
                     /locus_tag="ACHOQQ_RS11215"
                     /old_locus_tag="pgaptmp_002243"
     CDS             complement(6632..7045)
                     /gene="entH"
                     /locus_tag="ACHOQQ_RS11215"
                     /old_locus_tag="pgaptmp_002243"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGU4470484.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7048..7794)
                     /gene="entA"
                     /locus_tag="ACHOQQ_RS11220"
                     /old_locus_tag="pgaptmp_002244"
     CDS             complement(7048..7794)
                     /gene="entA"
                     /locus_tag="ACHOQQ_RS11220"
                     /old_locus_tag="pgaptmp_002244"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGU4470485.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(7794..8651)
                     /gene="entB"
                     /locus_tag="ACHOQQ_RS11225"
                     /old_locus_tag="pgaptmp_002245"
     CDS             complement(7794..8651)
                     /gene="entB"
                     /locus_tag="ACHOQQ_RS11225"
                     /old_locus_tag="pgaptmp_002245"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGU4470486.1"
                     /translation="MAIPKLQAYALPEAHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8665..10275)
                     /gene="entE"
                     /locus_tag="ACHOQQ_RS11230"
                     /old_locus_tag="pgaptmp_002246"
     CDS             complement(8665..10275)
                     /gene="entE"
                     /locus_tag="ACHOQQ_RS11230"
                     /old_locus_tag="pgaptmp_002246"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGU4470487.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAANNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFIAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10285..11460)
                     /gene="entC"
                     /locus_tag="ACHOQQ_RS11235"
                     /old_locus_tag="pgaptmp_002247"
     CDS             complement(10285..11460)
                     /gene="entC"
                     /locus_tag="ACHOQQ_RS11235"
                     /old_locus_tag="pgaptmp_002247"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGU4470488.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQLLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11649..12605
                     /gene="fepB"
                     /locus_tag="ACHOQQ_RS11240"
                     /old_locus_tag="pgaptmp_002248"
     CDS             11649..12605
                     /gene="fepB"
                     /locus_tag="ACHOQQ_RS11240"
                     /old_locus_tag="pgaptmp_002248"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4470489.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGIHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12609..13859)
                     /gene="entS"
                     /locus_tag="ACHOQQ_RS11245"
                     /old_locus_tag="pgaptmp_002249"
     CDS             complement(12609..13859)
                     /gene="entS"
                     /locus_tag="ACHOQQ_RS11245"
                     /old_locus_tag="pgaptmp_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGU4470490.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            13970..14974
                     /gene="fepD"
                     /locus_tag="ACHOQQ_RS11250"
                     /old_locus_tag="pgaptmp_002250"
     CDS             13970..14974
                     /gene="fepD"
                     /locus_tag="ACHOQQ_RS11250"
                     /old_locus_tag="pgaptmp_002250"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGU4470491.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14971..15963
                     /gene="fepG"
                     /locus_tag="ACHOQQ_RS11255"
                     /old_locus_tag="pgaptmp_002251"
     CDS             14971..15963
                     /gene="fepG"
                     /locus_tag="ACHOQQ_RS11255"
                     /old_locus_tag="pgaptmp_002251"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGU4470492.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15960..16775
                     /gene="fepC"
                     /locus_tag="ACHOQQ_RS11260"
                     /old_locus_tag="pgaptmp_002252"
     CDS             15960..16775
                     /gene="fepC"
                     /locus_tag="ACHOQQ_RS11260"
                     /old_locus_tag="pgaptmp_002252"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4470493.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(16772..17905)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOQQ_RS11265"
                     /old_locus_tag="pgaptmp_002253"
     CDS             complement(16772..17905)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOQQ_RS11265"
                     /old_locus_tag="pgaptmp_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGU4470494.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4229 a         5111 c         4681 g         3985 t
ORIGIN      
        1 tgttcagtcc ctgcgcgagt tgtacaccga tgtcatcgaa gagttttact gggttgcgct
       61 cccgcttacg acgcaaaatt ccctttcaca ataccaaccc gaatggcagt gctgggaacc
      121 tgatgtcgaa tgggtgcgtc agcccccgca agatgcgata accgatcctg actttttctg
      181 cttttaccag ccaggcatga ccttcgaaca atttgtacgt gaatttgccg aatggttttc
      241 acaaaaacgt ccggcggcga tgatgatcgg catccgtgcg gatgagtcct acaaccgttt
      301 tgtcgccatc gccagtttaa ataaacaacg ttttgccgac gataaaccct ggactacggc
      361 cgcaccaggc ggtcatagct ggtacattta ccccatttac gactggaaag tggctgatat
      421 ctggacctgg tatgcaaatc atcaaagtct ctgcaaccca ctgtataacc taatgtatca
      481 ggcaggcgtt cctctgcgtc atatgcgaat ttgcgaacct tttggcccgg agcaacgaca
      541 aggattatgg ctctatcacg ttatcgaacc ggatcgctgg gctgctatgt gcgcacgagt
      601 cagcggggta aaaagtggcg gcatttacgc cggacatgac aatcatttct atgggcatcg
      661 gaaaatcctc aagccagaac atttagactg gcaagaatat gcattattgc tgctcaatag
      721 catgccggaa aaaacagctg agcattaccg caataaaatc gccatttatt tgcactggta
      781 tcagaaaaaa ggcatcgagg ttccacaaac ccagcaaggg gacattggcg cgaaagatat
      841 cccctcctgg cggcggatat gcaaagtttt actcaataac gattactggt gtcgggcatt
      901 atcatttagt cctacgaaag cgaagaacta tcagcgttat aacgaacgga taaaaggaaa
      961 acgtcaggaa tgggggatac tatgcaacaa cgattaacgc aggatttaac gcaatttctc
     1021 gccagcttgc cggaagatga tcgcatcaac gcgattaatg aaatccgcat ggcgatccat
     1081 caggttagcc cctttcgtga agagccagtg gattgcgttc tgtgggttaa aaacagccaa
     1141 cttatgccta atgattacaa cccaaacaac gtggcaccac ccgaaaaaaa actgctgaaa
     1201 aaatccattg aaatagatgg ttttacccaa cccattgtgg tcacgcatac ggataaaaat
     1261 gccctggaaa tcgtggatgg ttttcaccgc catgaaattg gtaaaggctc atcatcatta
     1321 aaattacgct taaaaggtta tttgccggtg acctgtctgg aaggtacgcg taatcaacgc
     1381 atcgccgcga ctattcgtca taatcgcgct cgtggacgcc atcaaatcac cgccatgtcg
     1441 gaaatcgtcc gtgaacttag ccagttggga tgggacgata ataaaattgg caaagagctg
     1501 ggaatggaca gcgacgaagt gctgcgcctg aagcaaatta acggcctgca agagttgttt
     1561 gccgaccgtc aatattcccg cgcctggacg gttaaatagc taaagctggc gcaggcgttc
     1621 agctgctgcc agcaacgtcg attccttctt ggcaaaacag agacgaatca gtttatgagg
     1681 gaagggatcg gcgcaaaaca ccgatagcgg aatcgccgct acgccgtgct cctgcgtcag
     1741 ccactggcaa aactcaacat catccagggt agatacggcg ctgtaatcca ccagcaaaaa
     1801 gtatgtacct tcacacggta aaatctccag ccggctttca tttaaagcat tcaccagaat
     1861 atcgcgcttc tggcgataaa agtccggtaa cgcaagataa tgctcaggtt ctgcacgtag
     1921 catatcagca agcgccagct gtgccggggt attcaccgaa aaggtcagat actgatgtac
     1981 cttgcgaatt tcggcactga tgggcgctgg cgcaacacaa taacccactt tccagccggt
     2041 catatgatag gtcttgccaa atgaagaaac cgccactgcc cgctcacgca gctgcggatg
     2101 cgccagcaca ctggcatggc cctgttgtga aaagttgatg tgctcgtaga cttcatcgct
     2161 aatgacaaaa atctcgtgcc cggcgatcgc ctgccacaaa gcggcgaaat cagcctgctg
     2221 ccagacagtt gcactgggat tatgcggagt gttgaggatc accagtctgg tgcgctcgct
     2281 taacaatgcg gcaaattcct gccagtcaac gcgaaaatgc ggtggttgca gtgccatacg
     2341 cttcactatt cccccagaaa gcgcgatggc gggggcgtaa ctgtcatagc tgggatcaaa
     2401 acaaatcact tcatcgccat tgcgcaccag tgcggtaatc gccgcgtata acgcttccgt
     2461 cgcccctgcc gttacggtga tatcgctatc ggcatctggt tgatagccat acaaacgttc
     2521 cgttttctga gcaatcgcct cgcgcaaggc ctgcacgccg gtcatgggcg cgtattggtt
     2581 tgccccctgg gcaacgtggt acgccagccg ctcctgtaaa tagcgcggac catcaaaatc
     2641 aggaaagcct tgcgacaggt taatcgcctg gtgttgctgc gccagcgcgc tcatctgggt
     2701 gaaaatagtg gtgccaagtt gtggaagttt gctttgtgga atcagagggt tatttgtcat
     2761 ttttattgta cctgacgaag ttgcgtgttg aaggcactat aacacgatgc tagtatttgg
     2821 caatcaagac gtttagatgt ctaaataaaa caataaggac aacacaacat gcctcacaat
     2881 cctatccgcg tggtcgtcgg cccggctaac tacttttcac atccaggaag tttcaatcac
     2941 ctgcacgatt ttttcactga tgaacaactt tctcgcgcgg tgtggatcta cggcaaacgc
     3001 gccattgctg cggcgcaaac caaacttccg ccagcgtttg gacttccagg ggcaaagcat
     3061 attttgtttc gtggtcattg cagcgaaagc gatgtacagc aactggcggc tgagtccggt
     3121 gacgatcgca gcgtggtgat tggcgtcggc ggcggcgcac tgctcgacac cgcgaaagcc
     3181 ctcgcccgcc gtctcggtct gccatttgtt gccgctccga cgatcgccgc cacctgcgcc
     3241 gcctggacac cgctctccgt ctggtataac aatgccggac aggcgctgca ttatgagatt
     3301 ttcgacgacg ccaattttat ggtgctggtg gaaccagaga ttatcctcaa tgcgccgcaa
     3361 caatatctgc tggcggggat cggtgacacg ctggcgaaat ggtatgaagc ggtggtgctg
     3421 gctccgcaac cagaaacgtt gccgctaacc gtgcgactgg ggatcaataa tgcgcaggcc
     3481 attcgcgacg tcttgttaaa cagtagcgaa caggcgctga gcgatcagca aaatcaacag
     3541 ttaacgcaat cattttgcga tgtggtggat gctattattg ctggtggtgg gatggtcggt
     3601 ggtctgggcg atcgttttac gcgtgtggcg gcggctcatg ccgtgcataa cggtctgacc
     3661 gtgctgccgc aaaccgagaa gtttcttcac ggcaccaaag tcgcctacgg aattctggtg
     3721 caaagcgcct tgctgggtca ggatgatgtt ctggcgcaat taactggagc gtatcagcgt
     3781 tttcatctgc cgactacgct ggcggagctg gaagtggata tcaataatca ggcggagatc
     3841 gacaaagtga ttgcccacac cttgcgcccg gtggagtcca ttcattacct gccagtcacg
     3901 ctgacaccag atacgttgcg tgcagcgttc gaaaaagtgg aatcgtttaa agcctgacgt
     3961 gcaaatttag cagcagcgcg cgccgccttt tccaccgtag cgcgcgtcct gccgctcacg
     4021 gaaaaactcc tcgtaggtca tcggcgtttg atcgggatgg ttaacccgca tatgttcgac
     4081 atagttgtcg taatcaggca taccaatcat cagtttcgcc gcctgaccta aatattttcc
     4141 ggcttttgcc agtgaatcaa acatcttggc ctccgctaac catgttgctg aaagagtgcc
     4201 ggatgcggcg tgaacgcctt atccggccta caaatgctat caacgcgctc ggtcagtccc
     4261 ctcgccccat cggggagagg gttagggtga gggggaaccc acggcgccat gccaacattc
     4321 gccaacatcc cccctcactc tgactttagt gcgcgccttt tgcctgcgcc acgatctcct
     4381 cgacattttc cggcattggc tcatacggca tttctttcgc cgttggcttc ggctctttca
     4441 atgccgcaag tgccgtctta atcgagaaca gtgccagaac caccacgacc accataaaga
     4501 agatggttaa cccggcatcc agacggttgt tgaacaccag ttgcgccagc tgtgactcag
     4561 tatactgcga cggaatattg ccgctgtcga tcattgcctg gaacttgtta gcaatggcca
     4621 ggaagccgac tttcgcatcc gggctaaacg ctttctgcca gccagcggtc agggtacaaa
     4681 tcagcagcca ggccgttggt accagcgcca cccaggcgta acgttgacgt ttcatcttga
     4741 acagcaccac ggcacagagc atcagcgcca tccctgccag catctggttg gcaataccaa
     4801 acagcggcca cagagtgtta ataccgccca gcggatcgac cacgccctga tggaggaagt
     4861 agccccacgc tagcacgcac agcgccgttg ccagcaggtt agcaggcagt gaatcggtcc
     4921 gcttcaggcc aggagacacc acgcccagca gatcctgcaa cataaagcgc gcagcacgcg
     4981 tacccgcatc taccgccgtc agaataaaca atgcttcaaa caaaatggcg aagtgatacc
     5041 agaacgccac atccatcatg ccgcccagcg cgccgtgcag aatgtaggcc atccccaccg
     5101 ccagcgtcgg cgcaccgccc gcacgggaaa tgatcgactg ttcacccact tcgctggcaa
     5161 tctggtttag cgtatccggg gtaatcgcaa agccccagct actcaccact tgcgccgcag
     5221 aagcgaccac atccgccgtc cctgccggag ccagcaccgc catcgggctg ttcatggcga
     5281 aatagacgcc cggatcgatg atacaggcgg aaaccagtgc cataatcgcc acgaaggatt
     5341 ccatcaacat cccgccgtag ccgataaagc acgcctgccc ttcgttcgcc agcatcttcg
     5401 gcgtggtacc ggaagagatc agcgcatgga agccagacac cgcaccacag gcgatggtga
     5461 taaacaggaa cgggaacagg ttaccggtcc ataccgggcc agtgccatcg acaaatttgg
     5521 tcagcgcagg catggtcagc gtcggacgca taatcaaaat gcctaccgcc agaccgacga
     5581 tagtcccgat tttcaggaag gtagagaggt agtcacgcgg ggccagcagt aaccacaccg
     5641 gcagcaccgc cgccacaaaa ccgtagccca ccagcatcca ggtcagctgc acgccggtaa
     5701 agtcaaagta cggtgcccag gtcgggcttt ctgccaccca gccgccagag ataatggcaa
     5761 aaatcaggaa caccaggcca atcactgaca cttcgccaat acgccccgga cgcagatagc
     5821 gcaggtagat tcccataaaa atcgccagcg gaatggtgaa cgcaacggta tacgttcccc
     5881 acgggctatg agtcagggct ttcaccacga tcatcgccag tactgcaagg ataatgacca
     5941 tgatcataaa gcaggccacc agcgcgatca ccccggcggt tggccccatc tcttctttga
     6001 ccagctcacc cagcgaacga ccgtcacggc gcgtagaaac aaacagcacc atgaaatcct
     6061 gcaccgcacc ggcgagaacc accccggcga gtagccagat catccctggc aggtaaccca
     6121 tttgcgccgc cagtaccggc ccaaccaacg gacctgctcc ggcaatggcc gcaaaatggt
     6181 gaccgaacag cactttcttg tccgtcggca catagtccag cccgtcgtta tggcgcaccg
     6241 ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt ggcgatatac aggccataaa
     6301 aacggtaagc gatcagatag atacagaccg acgccaccac aatccacagc gcgttgatct
     6361 gttccccacg atttaaagca atgtatccca gagcaaatgc tcccattaca gagagcactg
     6421 tccagacgag gtatttccct gatttgttca tagttgttat ccgtgtgcgt gtccatagag
     6481 atgttacatt ttgtttctat aacatctttg tacgatttaa caacacggca accatgtaaa
     6541 tcggagttga aaccaatatt taacttaaca atgttaactc gatcactccg ttaaccgagt
     6601 tttcctgcaa tctcatttaa ttctgtcggg ctcatcccaa aactgccgta cccagccgac
     6661 aagtgcaaca acgccgcccc tgttcatcga aaacgacgat ttcccagctc tgattttgcc
     6721 gcccaagatg cagcggctgg cagacgccgc gtaccttgcc ttcagacacc gggcgatgat
     6781 gggtggcgtt aagttccgtg cccaccacac actgcccgtc acgggtcatc ataaatccgg
     6841 ccatcgatcc cagcgtttca gccagcgcag ccgacgcgcc gccatgcagc aggccaaacg
     6901 gctgatgagt acgggtatca accggcattt cggcttccag cacatcatcg cccagacggg
     6961 tatacacaat tcccagatgc gccaccattg tgttatcgct ggtggcgttc agttcgtcga
     7021 gcgttaaatg gcgtttccag atcatgctta tgcccccagc gttgagccgc catcgaccac
     7081 aatatcctgt agggtgatat ggctggcgag gtcagaggcg aggaacaaaa tcgtgttggc
     7141 gatctcttgt ggacgggcga ttttccccag cggaatgccg agtttaaact gctcgccaaa
     7201 gccgcgaata cgctgttctt cggcgtcatc gctcacccac agcgtgcgtt gcatatcggt
     7261 atcggtggaa cccggcgaaa ccacattaca gcgcacgccg ctacccgcca gttccagccc
     7321 gacgctcaac gccaggcttt tcagcgccgc tttcgatgcg ccataagcac tcataccaat
     7381 acgcggcgtg tgtgcggcgt cggacgccac agtgacaatc gccccgcccc gctgacggcg
     7441 aaactggttc atggtttgct ggaacaggtt aaacgcaccg ccgacgttaa ccgcaaaagt
     7501 ctgctgccag tcctccttac tgagctgatc ggtcgcgccc atgcgcaaaa ttcccgccgc
     7561 attgaccagc acgtccagtc gctccgtttc agctaacagt cgctgacata cctgcgccac
     7621 ctgcgcagca tcggcaacat ccattacttc ggtcgcaaag ggatattgct cctgagcgaa
     7681 cgcttgatca aaacctgtaa ctttcgctcc cgcttcaaca aacgccagcg ccgtggcgta
     7741 gccgatacct ttccccgcgc cggttaccca gacattttta ccgctgaaat ccattatttc
     7801 acctcgcggg agagtagctt ccaccaggcg tcgatggtcg ggtttttcgc caacatgaca
     7861 aagtcgatat cgccatgcac tttgcgccag cgcgccgcca gcgccatcat gcgcaccgaa
     7921 tccagaccgt agtcgatcag gttgtcgtca tcgaacggtt catcggactc gtccagcaac
     7981 ggcaggatca cctcacgcag cgccgctttg ctggcgggga taggtgctgg cagtaattct
     8041 tcggtcatca ccacccggcc agaacgtccg gccacatatt tcagcgacat caaatgctcg
     8101 tcacggctga aatcggccag cgcatccgcc accataaacg gtttaatatc gcgcataaat
     8161 gcgtcggttg cggtggtcat acagccaatg tgggcatata cccctgtaat aatcagctgg
     8221 ttacgtccac tctctttgag catttgctcc agcggagaac gatgaaacgc gctgtagcgc
     8281 cacttcacca gcaccgtgtc gtcggcatct ggcgtcaggc gatccaccac cttttgctgt
     8341 tccggcgagc gggtcaggcc cggcccccac atatcattca acagcgcccg atcttcatcg
     8401 ctctgctctt tcggctgggc ggtgtaataa accgggatat tgtgctgttt gcagtagtcg
     8461 cgcagcgcag caatattcgc gatcacctgc tccatcatcg ggcagttctc gccccagaag
     8521 ctgacaaaat agtcctgcat atcatggatt aacaacgcgg cacgttgcgg ttcaaaggcc
     8581 cagtcgactt tattcttagg gatatcgtga gcctccggca gtgcgtaagc ctgtaatttt
     8641 ggaatagcca tcgtgttctc tccttcaggc tgatgcgcgt gacgccagcc actgacgtaa
     8701 ttgtttttta tcgactttcc cgaccgccgt cagcggaagt gaatccacac actccacgcg
     8761 atccggtaat ttaaattcgg caataccctg ttcacgcagg aaacgacgca cctgcaccgc
     8821 gcgcagcggc tcttttacca ccagataagc acagcttttt tcgcccatca gctcatcttc
     8881 catactgacc agtgcggcgt agatcaccgc cggatggcgc agcaacaggt tttcgatctc
     8941 ttcggcagcg atcttctcgc cgccacggtt gatctgatct ttctcgcgcc cctgcacggt
     9001 gatgtaaccc tctggatcaa tagagatcag atcgccggaa cagtaaaaac cgttggcatc
     9061 aaaggcgctg gcattgtgct gcgggctttt gtaatagcca cggaaggtat acggcccgcg
     9121 cgtcatcagg cgtccgactt ccccttgcgg cagtggattt ccttcggcat cggcaaccca
     9181 tacttcgtca tccggacaca ttgggtaacc ctgggtatgg ataattttct ccgcgctatc
     9241 atcaagacgg gtatagttca ccagcccttc cgccatacca aacacctgct gcaactggca
     9301 accaatctca gcgggaatac gcgccgcaag cgtggcagaa agacgtgcgc caccgacctg
     9361 taacagtttc agcgaggcaa gctgcgcccg gctttcgcct tcggtcagcg cctgcaacca
     9421 caggctgact gccggcggca ccagcgcggt aacgttaatc tgatgttttt caatcaatgg
     9481 gaagcaaagc gtggcgctgg gatcggccgc cagaacaacc gttcctccgg caagaaagac
     9541 gcccagcgat cctggcgaac tcatggcgta gttatgagcc gccgggatcg cgcacaggta
     9601 gcgcgtctgt tgtgtgaact gacaaatctc gacgctacga cgcacgctgt agtagtagtc
     9661 gttatgagtg cgcgggatca gtttcggtgt gccggtggtg ccgccggaaa gctggaaata
     9721 ggccacttca tcagcaggtg acggcgtggc agtaaaatcc tcagccggat ggttaatcgc
     9781 atcctgcaag ttatgctcac cgctgtcgtt gtgcagttgc accacgcgaa tggaggagtg
     9841 ctctgcaata aatgtattga ggaaatcatc cccgctaaat agcgcatgtt ggcgatcggc
     9901 aatcagtaac gccggttcaa tctggctggc ataggcgttc agttcactac gctgatggct
     9961 gaacagcgcc agcaccggcg caacgcccag tttcagcagc gcgaaaaagg taatgtaaag
    10021 ttcagcgacg ttacccagtt gtaccagcgc ggtttcaccg ggtttaatgc cctgacggcg
    10081 caaactacag gcgagattat tcgccgcctg attcagctcc cgataactca actgccgctc
    10141 gccgtcgata accgcgatgc tgtcactcgc agcgtggcga gtcagaatgt cagtcagtgg
    10201 caaatcctgc cagtagcctt tttcccgata gcgacgggca aactcttccg gccagcgggt
    10261 aaatggaatg ctcatcatcg ctccttaatg caatccaaaa acgttcaaca tggtagaaag
    10321 tttgacgcct gtttcgcgcc actcacccaa cggtgacgac gcaggcacaa tccccgctcc
    10381 ggcaaacaga cgcacctgat tttcccgcag cttcgcgcag cggatggtca ccacccattc
    10441 gccgttacct tcgctgtcac accaacccac aatgccgcca aacagttcgc ggtcgaacgg
    10501 ctccagttca gcaataacct gggtcgcggc ctgatgtggg aaaccgctca gcgcgggggt
    10561 cggatgcagc agacaagcca gagtcagtgc gttttcttgc gaattcgctt taccttcaaa
    10621 gggagttgcg agatgccaca gcgtcggcgt ggtaatcaat tgtggagagg agggaacgtg
    10681 taactcacta ctgcgttcgc gcagtacctc tttcatcgcc tgagtcacca gttcatgttc
    10741 atggcgatct ttttctgacg ccagcagacg attacccgct tcgcgatcga gcacttcatc
    10801 cggctgacga cgcgcggaac cggctaacgg aatggagcta aaacgctcgc cgtctttacg
    10861 tagcagcagt tccgggctgg cccccagcag aacgccacca tctgccaacg gcacatggaa
    10921 gttgtaacta accgggtttt gcgcaatcaa ccgttccagc aatacgccac tatcaatggc
    10981 ggcgtcagtg gtgatatcaa tcaaccgtga caacaccact ttgtcgacct gcggcgtggc
    11041 ggtaagtgcg gcggcgcggg caaccatctg ttcaaacgtg gtttgttccg gaattgcctg
    11101 gcgttccacc acattcagca actggctgcg ggtgaaacgg cgtgcggaag cttgtttttc
    11161 ctgacgggag aacgactgcc aggattcggg aatatacagc gacgaaggct gacgtggatc
    11221 gaagggaatc gccccgacca tcaccggatt tttgatgccc tgcgctttgg catcggcaaa
    11281 cagcgcggcg agtttttgct ggaagggact gtcaggcgaa tccccgttca cagccggttc
    11341 atcgaagcgg gcgaaacatc ctgacgtcgt aaaactgcgg tacggcgaca taaagaaaaa
    11401 gcgattgggc gcaagtgttg ccatggtctg ctgtacttcc tcagccagtg acgtatccat
    11461 atcatcctcc acaaaatgat aaaggcttta ataatgatta tcatttatat tttcggtcgc
    11521 taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt aacgcaatct gcaactcggg
    11581 gttgcatctg gttgtggtta caatgaaaat gagaagcatt aacttcatta ataacaggaa
    11641 gctgatttgt gagactcgcc ccgctctacc gcaatgccct tctattaaca ggacttttgc
    11701 tttcaggaat agccgcagtt caggccgccg actggccgcg tcagattact gacagccgtg
    11761 gcattcatac cctggaaagc cagccgcagc gtattgtttc caccagcgtc accctgaccg
    11821 gctcactgct ggcgattgat gctccggtga tcgccagcgg cgcgaccacg ccaaataacc
    11881 gcgtcgcgga tgaccagggc tttttacgcc agtggagcaa ggtggcgaaa gaacgcaaac
    11941 tgcaacggct ctatatcggc gaaccgagtg cagaagccgt tgccgcgcaa atgccggatc
    12001 tgattttaat tagcgcaacc ggcggtgatt cggcgctggc gctgtacgat cagctttcca
    12061 ccatcgcgcc gacattaatc atcaattacg acgacaaaag ctggcagtcg ctgttaacgc
    12121 agcttggcga aattaccggg catgagaaac aagcggcaga gcggattgca cagtttgata
    12181 agcaactggc ggcggcgaaa gagcaaatca aattaccgcc gcagccggtc actgccattg
    12241 tctataccgc cgctgcacac agtgccaatc tctggacgcc ggaatcagca caagggcaga
    12301 tgctggaaca actcggcttt acgctggcga agttgcccgc aggcttaaac gccagccaaa
    12361 gccagggcaa acgccatgac atcattcagc ttggtgggga aaatctggct gcagggttaa
    12421 atggcgagtc actattcctg ttcgccggtg atcagaaaga tgccgatgct atttatgcta
    12481 atccgctgct cgcgcacctg cctgcagtac aaaacaagca ggtttatgcg ctgggaaccg
    12541 agacgttccg tctggattac tacagcgcca tgcaagtgct ggaacgactt aaggcgctgt
    12601 tttaagcatt aaccgtcaga cgctgtcacc tgcggcggcg tctggcgaaa acgtcgcaac
    12661 tccaccagca ccagcaataa caacacgccg ataatcaaca aaccaaaacc gcttgcgctt
    12721 gcggaagcaa ccggtgtcat catcgctccc aaaccgccca gcagcgccgc gcctatggca
    12781 tcgcccgtca cgttttgtgc cgtccacaaa ccgttaatcc gccctaacat cgcttccggg
    12841 gtttgcgttt gcagcattgt gtattgcagc aacgagctga ccgcactcaa ccagccgaac
    12901 agcgccagac aaaccacgcc taaaatccac atcggcatca ggccaaacag accaatggcg
    12961 aggaacgatc ccagcgtgga gagcagcatc aataaccctg gtcgcgcgct atgcgccagc
    13021 ttcccgctgg ttaacgcgcc aatagccgcg ccgagcggga tcgccgcata gagaaaacca
    13081 atctgtgccg ccgacatctg ccagttgtca gccagcgccg gatacagtac ccgcaccgca
    13141 ctcgccatcg tcaataaacc acccagcagc gcaatccctc ctaccagcgg gctggcgagc
    13201 agaaaacgaa atcctgccag taatgatttc aacggatgct cacgcggctg cggtggcggt
    13261 ggcaacgccg gaaggcttaa caacggcagc aaggtaataa acgtgcccgc cgccgccagt
    13321 ccgtagttcc aggccacacc gccagtcgcc agcagtaaac cgccaatcat gggcgaaatc
    13381 accgacccca gacgcacggt caacatggtg atcgccccgg cctgcattaa gttttcacgc
    13441 cctaccagtg caggtgtcgc cgccagtagc gccgtaacgc caagtgatgc gaaaaaacca
    13501 tcccataaac caagtaaata gattgccagc aatgacggtt caggcagcag tgcattaagg
    13561 cacagtccaa tgaagccaat gccacaggtg ccgcgcgcca gcaaaatcac ttttttgcgt
    13621 tcatagcgat ccgccagcac accgccgacc atcaggccaa caaacatcgc gccgccggtc
    13681 agcgtcaccg aaagccccac ctgccaggtg gaatgtgtca tcatctggat ctgcaccggc
    13741 accgcgacgc cgagcaaacc cagagaaaca atagagatga aacgagcgag gaatactgcg
    13801 cgaaacgccg ggtgcgtttt caacaggctg aggttaagca gccaggattg tttattcatt
    13861 acaatgcctt gccatcaaat attttaaata cctattctta ggctgcacat gctaacatat
    13921 ccaaataaga tcgataacga taattaattt cattatcatg gaagttcgta tgtctggttc
    13981 tgttgccgtg acacgcgcca ttgccgtgcc cggattgctg ttattactga ttatcgcgac
    14041 ggcattaagt ctgctcattg gggcaaaatc actccccgct tccgtagtgc tggaggcctt
    14101 ctccggcacc tgccagagcg ccgactgcac catcgtgctc gacgcgcggc tgccgcgtac
    14161 ccttgccggt ttactggcag gcggcgcgct tggccttgcc ggggcgttaa tgcaaaccct
    14221 cacccgaaac ccacttgccg accccggctt gcttggcgtg aacgccggag ccagctttgc
    14281 cattgtgctg ggtgcggcgc tgtttggtta ctcttccgca caggaacaac tggcgatggc
    14341 cttcgccggg gcgctggtgg cctcgttgat tgttgccttt accggcagcc agggcggcgg
    14401 gcagttaagt ccggtgcgtt taaccctggc gggcgtggcg ctggcggcag tgctggaagg
    14461 gctgaccagc ggcatcgccc tgcttaatcc tgacgtctac gatcagttgc gtttctggca
    14521 agccggttcg ctggatattc gcaatctaca taccttaaaa gtggtgctga tcccggtgct
    14581 gatcgccgga gcaaccgcgc tattactgag ccgcgcgctg aacagtttga gcctcggcag
    14641 cgacaccgcg acggcgctgg gcagtcgcgt ggcgcgcaca cagttgattg gtctgctggc
    14701 gattaccgtg ctttgtggta gtgcgacggc ggtagttggc ccgattgcct ttatcggcct
    14761 gatgatgccg cacatggcgc gctggctggt aggtgccgat catcgctggt cgctgcccgt
    14821 cacgctactt gctacccctg ccctgctgct gtttgccgat atcatcgggc gggtgattgt
    14881 gcccggcgaa ctgcgcgttt ctgtggtcag tgcgtttatt ggtgcaccgg tgctgatctt
    14941 cctcgtgcga cgtaaaacgc gaggtggtgc atgatttacg tctctcgccg attactcatc
    15001 acctgtttgc tgctggtttc cgcctgtgtg gttgcaggta tctggggatt acgcagcggt
    15061 gccgtcacgc tggaaacctc gcaggtattc gccgcgctga tgggcgatgc gccgcgcagt
    15121 atgacgatgg tggtcaccga atggcgttta ccacgcgtgc tgatggcgct gttgattggc
    15181 gcagcactgg gcgtcagtgg cgcgattttt cagtcgctga tgcgtaaccc gctcggcagc
    15241 cctgacgtaa tgggttttaa caccggggcg tggagcggcg tgctggtggc gatggtgctg
    15301 tttggtcagg acctgacggc tatcgcgctg gcagcaatgg tgggcggcat tatcacttcg
    15361 ctgttggtct ggctgctcgc ctggcgtaac ggtatcgaca cctttcggtt gattattatc
    15421 ggtatcggcg ttcgcgccat gctggtggcc tttaatacct ggctgttgct gaaagcgtct
    15481 ttagaaacgg cgctaacagc aggtttgtgg aatgccggat cgctcaacgg cctgacgtgg
    15541 gcaaaaacct cgccttccgc acccatcatt atattgatgc taattgccgc cgccttactg
    15601 gtacgccgga tgcgcttgct ggaaatgggc gatgataccg cgtgtgcgct gggcgtcagc
    15661 gtcgaacgtt cgcgtctgtt aatgatgctg gttgcagtgg tgcttaccgc tgcggcaaca
    15721 gcgcttgccg ggccgatttc ctttattgct ttagtcgcgc cgcacattgc ccgacgcatt
    15781 agcggcaccg ctcgctgggg gctaacccag gcggcgctgt gcggggcgtt gttactgctg
    15841 gcggccgatc tctgcgccca acaactgttt atgccgtatc aacttccggt tggcgtcgtt
    15901 accgtcagcc tcggcggtat ttaccttatc gtcttgttaa ttcaggagtc tcgcaaaaaa
    15961 tgaccgaatc agtagcccgt ttgcgcggcg aacagttaac cctgggatat ggcaaatata
    16021 ccgttgcgga aaatctgact gtagaaatac ctgatggtca cttcacggca attatcgggc
    16081 caaatggctg cggtaaatcc acgttgctgc gtaccttaag ccgcctgatg acgcctgctc
    16141 acgggcatgt ctggctggat ggcgagcaca ttcaacatta cgccagtaaa gaagttgcac
    16201 gccggattgg tctgttggcg caaaatgcta ccacgccggg cgacatcacc gtgcaggagc
    16261 tggtggcgcg tgggcgttat ccgcatcaac cgctgtttac ccgttggcgc aaagaagatg
    16321 aagaggcggt aacgaaagcg atgcaggcca cgggaataac tcatcttgca gatcaaagcg
    16381 tggataccct ttccggtggg caacgccagc gagcgtggat cgcgatggtg ctggcccagg
    16441 aaacggcgat tatgctgctc gacgaaccca cgacctggct ggatatcagt catcagattg
    16501 atctactgga actgttaagc gaactgaacc gcgagaaagg ctataccctg gcggcggtgc
    16561 tgcacgatct taatcaggcc tgtcgttacg ccagccattt gattgcattg cgggaaggga
    16621 aaattgttgc tcagggagcg ccgaaggaga ttgtcactgc tgaattgatc gagcgcattt
    16681 atggtctccg ctgcatgatt attgacgatc cggtagccgg aacgccgctt gtggtgccgc
    16741 tcggacgaac ggcaccttca accgcaaaga tttaaactaa gtggtctgcc atcatggcat
    16801 cctgctttct ggatgccatc gcatagcgca ataacacgct accacaagcc accatcccgc
    16861 cgatcagcgc ggaaaggatc acaataatcg ccttacccgg accgtctttt ttcactggca
    16921 atgacgggct taactgatat ttaaacggcg taaaattcac atcgttgata tttgcttttg
    16981 ttaactgctc aacaagatac tgacgattac gtaattcacc gttcagttcc gcaacgtcag
    17041 tgaccgcttt ttcaatttcc agtttgcgtt caataccgtc tgcgccgaga gaaatagaga
    17101 aatcggggtc atctttaacg gcctgaccat tactgtaaac gggcttttta attcctgccg
    17161 cgttggcaat gtcgagtgaa taattgaggc gctgaatgtt tgcatcaagt tgatttttca
    17221 ttttaatgcg gtcctgagcc agtttttctt tttcaaactg ggttttgatc tccagtttat
    17281 tacggacgtt ttctatcgac tctttcacca ccaacgcaga gatataatcg atatacccgc
    17341 tcaaaacggt ctgcgcctct tcactggttg gcgcggtaaa acttagcgtc caggaggtat
    17401 acagtgacgg ttcatctttt ttcttactgg cattgtcatc aaccgctttc attttttcgc
    17461 tcaatgcgac aattgcgcga tgcaaatcca gttcgtcgat tttcgcctct tttaattggt
    17521 ccatcacata aggtgatgaa cgcaggtact cttccagcaa gctaaccgac tgaaacttct
    17581 tgataaacag gttaaatgct tctgtacgat caattttgat atccagatcc agcacacgaa
    17641 gcttagtgaa tgttttctcc aactcttgcc actgaacagg ttctggaggc gtgacaaccg
    17701 ccgcgctggt ccatttttgc ggcaggatga aagagatcag caagcctgcg caggcaaacg
    17761 caaaaacgac cgccatgacc gtttttttgg cccgccataa aactgagatt agattaagta
    17821 aatcaatttc attattactg ggcgacgcca gaggataatc gggaaaatga gcgtcacttc
    17881 cctgtttaat attcagtgat gacatggggt ataacctgaa aatgagtcca atagggggaa
    17941 atctccacgg cgctcacttt accagttggt gagaattatc cgaagctgaa gtttgtaaat
    18001 tcattc
//
LOCUS       DAZKFU020000084        17457 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZKFU020000084 DAZKFU020000000
VERSION     DAZKFU020000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.085"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            158..1369
                     /gene="mhpT"
                     /locus_tag="ACHOQQ_RS11270"
                     /old_locus_tag="pgaptmp_002254"
     CDS             158..1369
                     /gene="mhpT"
                     /locus_tag="ACHOQQ_RS11270"
                     /old_locus_tag="pgaptmp_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HGU4470495.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLTSLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            1471..2010
                     /locus_tag="ACHOQQ_RS11275"
                     /old_locus_tag="pgaptmp_002255"
     CDS             1471..2010
                     /locus_tag="ACHOQQ_RS11275"
                     /old_locus_tag="pgaptmp_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGU4470496.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(2236..3069)
                     /gene="fghA"
                     /locus_tag="ACHOQQ_RS11280"
                     /old_locus_tag="pgaptmp_002256"
     CDS             complement(2236..3069)
                     /gene="fghA"
                     /locus_tag="ACHOQQ_RS11280"
                     /old_locus_tag="pgaptmp_002256"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4470497.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(3162..4271)
                     /gene="frmA"
                     /locus_tag="ACHOQQ_RS11285"
                     /old_locus_tag="pgaptmp_002257"
     CDS             complement(3162..4271)
                     /gene="frmA"
                     /locus_tag="ACHOQQ_RS11285"
                     /old_locus_tag="pgaptmp_002257"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HGU4470498.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(4306..4581)
                     /gene="frmR"
                     /locus_tag="ACHOQQ_RS11290"
                     /old_locus_tag="pgaptmp_002258"
     CDS             complement(4306..4581)
                     /gene="frmR"
                     /locus_tag="ACHOQQ_RS11290"
                     /old_locus_tag="pgaptmp_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGU4470499.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(4741..5787)
                     /gene="fbpC"
                     /locus_tag="ACHOQQ_RS11295"
                     /old_locus_tag="pgaptmp_002259"
     CDS             complement(4741..5787)
                     /gene="fbpC"
                     /locus_tag="ACHOQQ_RS11295"
                     /old_locus_tag="pgaptmp_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HGU4470500.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADTA"
     gene            complement(5799..7877)
                     /locus_tag="ACHOQQ_RS11300"
                     /old_locus_tag="pgaptmp_002260"
     CDS             complement(5799..7877)
                     /locus_tag="ACHOQQ_RS11300"
                     /old_locus_tag="pgaptmp_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4470501.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFSEATREQRSRHYFDAGWALLCMAFIVIS
                     ATVVGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(7946..8977)
                     /locus_tag="ACHOQQ_RS11305"
                     /old_locus_tag="pgaptmp_002261"
     CDS             complement(7946..8977)
                     /locus_tag="ACHOQQ_RS11305"
                     /old_locus_tag="pgaptmp_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4470502.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(8974..10290)
                     /gene="uhpC"
                     /locus_tag="ACHOQQ_RS11310"
                     /old_locus_tag="pgaptmp_002262"
     CDS             complement(8974..10290)
                     /gene="uhpC"
                     /locus_tag="ACHOQQ_RS11310"
                     /old_locus_tag="pgaptmp_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU4470503.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(10363..11904)
                     /locus_tag="ACHOQQ_RS11315"
                     /old_locus_tag="pgaptmp_002263"
     CDS             complement(10363..11904)
                     /locus_tag="ACHOQQ_RS11315"
                     /old_locus_tag="pgaptmp_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU4470504.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQIVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMHERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(11904..12533)
                     /locus_tag="ACHOQQ_RS11320"
                     /old_locus_tag="pgaptmp_002264"
     CDS             complement(11904..12533)
                     /locus_tag="ACHOQQ_RS11320"
                     /old_locus_tag="pgaptmp_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGU4470505.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRVQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            12836..13798
                     /gene="tauA"
                     /locus_tag="ACHOQQ_RS11325"
                     /old_locus_tag="pgaptmp_002265"
     CDS             12836..13798
                     /gene="tauA"
                     /locus_tag="ACHOQQ_RS11325"
                     /old_locus_tag="pgaptmp_002265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4470506.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            13811..14578
                     /gene="tauB"
                     /locus_tag="ACHOQQ_RS11330"
                     /old_locus_tag="pgaptmp_002266"
     CDS             13811..14578
                     /gene="tauB"
                     /locus_tag="ACHOQQ_RS11330"
                     /old_locus_tag="pgaptmp_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HGU4470507.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            14575..15402
                     /gene="tauC"
                     /locus_tag="ACHOQQ_RS11335"
                     /old_locus_tag="pgaptmp_002267"
     CDS             14575..15402
                     /gene="tauC"
                     /locus_tag="ACHOQQ_RS11335"
                     /old_locus_tag="pgaptmp_002267"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGU4470508.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            15399..16250
                     /gene="tauD"
                     /locus_tag="ACHOQQ_RS11340"
                     /old_locus_tag="pgaptmp_002268"
     CDS             15399..16250
                     /gene="tauD"
                     /locus_tag="ACHOQQ_RS11340"
                     /old_locus_tag="pgaptmp_002268"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGU4470509.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPLQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(16357..17331)
                     /gene="hemB"
                     /locus_tag="ACHOQQ_RS11345"
                     /old_locus_tag="pgaptmp_002269"
     CDS             complement(16357..17331)
                     /gene="hemB"
                     /locus_tag="ACHOQQ_RS11345"
                     /old_locus_tag="pgaptmp_002269"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGU4470510.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         4144 a         4643 c         4758 g         3912 t
ORIGIN      
        1 aagcgtcgca tcaggcatct gcacacgact gccggatgcg gcgtgaacgc cttatccggc
       61 ctacatttcg cccgtaggca gtcattaaat agttctgatt acgcggctgg caatgtatca
      121 gtcgcgattc acctgcactc gcaacgaggt tcatcttatg tcgactcgta ccccttcatc
      181 atcttcatcc cgcctgatgc tgaccatcgg gctttgtttt ttggtcgctc tgatggaagg
      241 gctggatctt caggcggctg gcattgcggc gggtggcatc gcccaggctt tcgcactcga
      301 taaaatgcaa atgggctgga tattcagcgc cggaatactc ggtttactac ccggcgcgtt
      361 ggttggcgga atgctggcgg accgttatgg tcgcaagcgc attttgattg gctcagttgc
      421 gctgtttggt ttgttctcgc tggcaacggc gattgcctgg gatttcccct cactggtctt
      481 tgcgcggctg atgaccggtg tcgggctggg ggcggcgttg ccgaatctta tcgccctgac
      541 gtctgaagcc gcgggtccac gttttcgtgg gacggcagtg agcctgatgt attgcggtgt
      601 tcccattggc gcggcgctgg cggcgacact gggtttcgcg ggggcaaact tagcatggca
      661 aacggtgttt tgggtaggtg gtgtggtgcc gttgattctg gtgccgctat taatgcgctg
      721 gttgccggag tcggcggttt tcgctggcga aaaacaggct gcgccaccac tgcgtgcgtt
      781 atttgcgcca gaaacggcaa ccgcgacgct gctgctgtgg ctgtgttatt tcttcactct
      841 gctggtggtt tacatgttga tcaactggct accgctgctt ttggtggagc aaggattcca
      901 gccatcgcag gcggcagggg tgatgtttgc actgcaaatg ggtgcggcga gcgggacgtt
      961 aatgttgggc gcattgatgg ataagctgcg tccagtaacc atgtcgctac tgatttatag
     1021 cggcatgtta acttcgctgc tggcgcttgg aacggtgtcg tcatttaacg gtatgttgct
     1081 ggccggattt gtcgcggggt tgtttgcgac aggtgggcaa agcgttttgt atgccctggc
     1141 accgttgttt tacagttcgc agatccgcgc aacaggtgtg ggaacagccg tggcggtagg
     1201 gcgtctgggg gctatgagcg gtccgttact ggccgggaaa atgctggcat taggcactgg
     1261 cacggtcggc gtaatggccg cttctgcacc gggtattctt gttgctgggt tggcggtttt
     1321 tattttgatg agtcggagat caagaatgca gccgtgtgcc gatgcctgac gcgccttatt
     1381 aagcaggggc gaaagggact tgcccccaat acacccacgg cgtttacagg tatactcgct
     1441 aaaaattatt cagcgggttt ggaaacaaag atggcaaaac ttaccttaca agagcagttg
     1501 ctcaaagcag gattagtcac cagcaaaaaa gcggcgaagg tcgagagaac ggcgaaaaaa
     1561 tcgcgcgttc aggcgcgtga agctcgggcg gcggtagaag aaaataaaaa ggcacagctt
     1621 gagcgtgata aacagcttag cgaacagcaa aaacaagcgg cgttggcaaa agaatataaa
     1681 gctcaggtga agcagcttat tgaaatgaac cggatcacca ttgccaatgg cgatattggt
     1741 tttaacttca ctgatggcaa tctgattaag aagatttttg tcgataagct cacacaagcg
     1801 cagttgatta atggtcgtct ggcgattgcc cgcttgttgg tcgataacaa tagcgaaggt
     1861 gaatacgcca ttattcccgc cagcgttgcc gataaaattg ctcagcgtga tgccagcagt
     1921 attgtgttgc acagcgcgct cagtgccgaa gagcaggatg aagacgatcc gtatgccgac
     1981 ttcaaagtgc ctgatgattt gatgtggtaa acatagataa gccgtgcgca aatgcccgat
     2041 gcgacgctat gcgcgtctta tcatgcctat ggtttatggg cgaagtgtag gccggataag
     2101 gcgttcacgc cgcatccggc agccgtgcgc agatgcctga tgcgacgctt acgcgtctta
     2161 tcatgcctac ggtttatggg cgaagtgtag gccggataag gcgttcacgc cgcatccggc
     2221 agtcgtgcac tattatcaac gcatattcag tttattggcg tggtaggcaa tatgctcgcc
     2281 aataaaactg gagacaaaat aatagctgtg gtcgtaaccc tcgtgataac ggattaacgt
     2341 cttgatattc atctcctggc agatcttttc aagatttgga gtccgcagct gttctgcgta
     2401 aaaatcatca ctcaacccct gatcaaccat gatttccgca acgcgttgac cttgtgaaat
     2461 aagactcacc ggatcgtaat ccaaccaggc atctttattt tcaccaagat atgcggcaaa
     2521 ggcttgctgt ccccacggta cttgcgatgg ggagacaatg ggcgaaaacg ccgagacgct
     2581 gacatattca tctgggttac gtaacgccag caccagcgcg cccagtccgc ccatagaatg
     2641 accagagata gactttttgg ccgttgccgg aaaatgattc atcactaaat ccggcagctc
     2701 gttgcggata tagtcataca ttttgtaatg ttcattccac ggcgcttgcg tcgcgttcag
     2761 gtaaaacccg gcaccttgcc cgagatcgta acggtcagca tctgcgacat gactgcctcg
     2821 cggactggtg tccggcgcaa caacaataat gttgtgctca gccgcgtaac gctgcatccc
     2881 cgatttagta atgaaattct gctcgttgca ggtcaggcct gaaagccagt acagcaccgg
     2941 caatttttca ttcgcggctt ttggtgggag atagacgccg acattcattt cacatttcag
     3001 tgatgcggaa taatgccgat acacattttg ccagccgcca aagctggcat gtttttcaat
     3061 gagttccatc agtcattatc tcacggttaa tcccccagac ggctgcccgg ggagagtatt
     3121 aagaaccccg tctgaagtgg acggggtaaa ccagcgggaa atcagtaacg aattacggtt
     3181 cgaatggatt tgccttcatg catcaggtcg aaggcgtcat taatttcatc caggctcatg
     3241 gtatgcgtga caaacggttc cagatcgata tcacctttca tcgcatcttc aaccataccc
     3301 ggtaactggg aacgaccttt cacgccgcca aacgcggaac ctttccatac gcgaccagtg
     3361 accaactgga atggacgggt ggagatttcc tgaccggaac ccgcgacccc gatgatcacc
     3421 gactgacccc agccgcggtg cgcactttcc agcgccgcac gcatcacgtt gacattaccg
     3481 atgcattcaa aggtatggtc gataccccat ttgttgatat ccagcaggac atctttaatc
     3541 ggtttgtcgt agtcattcgg gttaatgcag tcggtggcac cgaagcgacg cgccagatcg
     3601 aatttcttcg ggttggtatc gatagcgata atccgtcccg ctttcgcctg acgcgcgccc
     3661 tgaaccactg ccagaccaat cgcgccaaga ccaaatacgg caacagaatc acctggctgg
     3721 actttagctg tgttgtgtac cgcgccaata ccggtggtca cgccacagcc cagcaggcag
     3781 acgtgttcat ggtttgcttc tggattaatt ttggccagag acacttccgc gaccacggtg
     3841 tattcactga atgtagagca ccccatgtag tgataaagcg gctgcccgtt gtaagaaaaa
     3901 cgggtggtgc cgtctggcat caggccttta ccctgggttt cgcgaaccgc aacacagagg
     3961 ttagttttgc cagaacgaca gaactcacac tcgccgcact ctgcggtgta aagcgggatc
     4021 acatggtcgc ctggtttgac gctggttacg ccttcaccga cttcaaccac aacgccagcc
     4081 ccttcgtgac cgagaaccac cgggaataca ccttccgggt catcgccgga gagggtaaat
     4141 gcgtcggtat ggcaaacgcc ggtatgggtg actttaatca gcacttcacc ttttttcggt
     4201 ggtgcaacgt caatttcaac gatttccagc ggtttaccgg gagcaaatgc aacggcagca
     4261 cgtgatttca tctctcgctc ttcctcaata tagtaataga ttcagctatt taagataggc
     4321 acgaaccagt tcaatagtgt cgtcaacgga ttggctgact tcgcggctgt agcagtcatt
     4381 tcggtcaaac gtttcccgga tatggctttc aagcacttct gccatcagcc cattagccgc
     4441 gccccgaacg gcagcgatct gttggagtat ggcacggcat tcggcatcac cctccagcga
     4501 ccgttccaga gcatcaatct gcccccgaat acgacgaact cgagtaagga cctttttctt
     4561 ctcttccgga gtactgggca tttcgcacct catcatctgc acgcaatata ctataggggg
     4621 gtatgctata tgtcaatgca taccccccta tagtatataa cactggagaa taattgttgt
     4681 ccgaagccta accaggtgaa cggtccctca agacagatgg ggaccgttta tgtgctattt
     4741 ttatgccgta tccgctaaaa caaacatccc gtaaggatgg atttcgaggt aatagtgctc
     4801 accaatatcg ggctgtaaac gggtggcgtt tacttgtaat aaaatctcct gtccatgcca
     4861 ctctacggtc acttcgtact gcggccccat gtaggcgaca tggcgaataa cacaacgctg
     4921 actctcttcg ccgtgattgc ttaaggtgat ggcttccggg cgaacgccga cggtgccgct
     4981 accttgcgcc gggaaatgcg ccgcgcgcgg caggcgataa ccgtagatat cgacatactc
     5041 ttcgctaaag ttcgccggga acaggttggc gtcgcccata aaacttgcca taaatcggga
     5101 ggctggctga cgatagagat cctgcggtga gccaatctgc atgatgtgac ctttattcat
     5161 taccagcaca gtgtcggaca ccgcgaaagc ttcactttga tcgtgagtga cgtagagcga
     5221 cgtgatatta aactgctttt gcagctcgcg gatcttatcg cgcatactgc ggcgcaggtt
     5281 ggcatcgagg ttacttaacg gctcatcaaa tagcagcacc tttggcttga ggatcaacgc
     5341 gcgggccagt gccacacgct gttgctgacc accggaaatc tgatcgacat agcggtcctc
     5401 gaagccttcc agatccacca ttgccagcgc ctccttcacc cgctgtttca cttcgctacg
     5461 cgacacgcca agcatcttca gcccgtagcc aacgttttca cccagcgaca tatgcgggaa
     5521 cagagcgtat gactgaaaca ccatgcagat atcgcgctgt tgaatggaac gatgcgtgac
     5581 atcttcgcca tcaataaaaa tttgcccttc gctcggtttt tccagcccgg caaccagacg
     5641 caaaatggtg gttttgccac agccggaagg accgagcagc gtcaccattt gcccttgtgg
     5701 aatggtgaga ttgatattgt cgataaccgt attactgccg aatcgtttag tgacgttgcg
     5761 cagttcaaca aaatttttct gactcatagt gcgctccatt acgcctggtt tttggcttta
     5821 gaacgggaga tacgagcttc tccgatcagc cagtcaaaga ggaaaataat cgccagcatc
     5881 accacgatca gaatcgaacc ataggcgatg gctacgccat attcgccgtc ttcaacgcgg
     5941 ttaagaatgt aggcggttgc cacgcgggtg tccggcgtga cgagaaagac gatggcgctg
     6001 accgtcgtga tggcccgcac aaagctgtag atcagggccg agagaatggc cggacgcagc
     6061 agcggcagta aaatgtgggt tatggttcgc aaggaacccg cgcgcaggct gagtgaggct
     6121 tcatccagcg atttgtcgat ctgccccaga cccgcaatcc cggcgcgaat acccaccgga
     6181 acgttacgca tcaccatcga gataatcaca attgccgccg ttccggtgag ataaaccgga
     6241 gcgctgttaa aggcgagaat atacgagacg cccgccaccg tacccggtac ggcaaagcac
     6301 agcatggtgg tgaactcgat ggtctttttg cctttgaatt gttgacgcac caccacccag
     6361 gcgatgagca ggccaaagat agcggtaatg ggagcggcaa tcccggcgta gagcagggta
     6421 tccagcagtg aaggccacgc gccgtcgctc attccctgac caaatagctt gataaagtta
     6481 tccagcgtca gggtgtagtc cacgccccag ttcaccgtga agctgccgta gaaaatgctg
     6541 ccgtagagca gggcgttaaa ggcaatccat atcgcaagta tggcgaccac gctccacacc
     6601 agggtgaccg gtagcggctg gacgtcgcca cggtaggatt tgccggaaac cgtgacgtaa
     6661 gagcgtttac caatccacat atactggatg cagaacacca gcagcgagaa cagcagcagg
     6721 aaagcgccaa gggtgctggc ggcctgataa tccagctgcg agccggtaat atagaagtaa
     6781 atttgcgtcg ccagtacgtc gaagttaccg ccaagtacca gcgggttact gaaatcagcc
     6841 agtgactgta ccacgacgat taaaaaggca ttggcgagtg cgggcttcaa caacggcaca
     6901 aacacgccgt taaaggtttg ccagcggctg gcgcgcaggg tgtaggaggc ctcttccagc
     6961 gagggatgaa tggttttaat ggccccgtcg aggatcataa atgccattgg cgtgaatgcc
     7021 agaacctgtg ccagccagat cccggtaaaa ccgtacagcc agttggtgtt ggtcagccca
     7081 aaccactcca ccataaattc cgtaacatag ccggagcgcc ccatcatgag cgtcacgcct
     7141 aagccgacca caaacggtgg cgtaacgatg ggcaaaatgg aaaagatgcg gccaataatg
     7201 gcgctgcgtt tagcgatgcg cgtggtataa atcgccagca ccaggccgaa gaaagtacag
     7261 cctatcccca ccgctatcga caagacgata gagttgacga tcacctgaac aatatgtgcc
     7321 tgcgagagca cggtcataaa cgccagcggg gcgaactctc ccgcgtcgtt ggtgaacatc
     7381 gggataaaaa tggcgatgct cggccagacg ataaaaatgc caatcagcgc cacaatcgca
     7441 atcagtgagc cgatcacgaa gcggtcgcca cccagccatt cgaggcgggt taaggccagc
     7501 gtcataatcg cgcctagttc gacaaacagc acgatggcgg cgtagcctaa accgcgtcca
     7561 accacggtgg cgctgatgac gataaacgcc atacagagca atgcccagcc ggcatcgaaa
     7621 taatgacggc tacgttgttc gcgcgtggcc tcactaaacg ggcggaccag caacaggctc
     7681 gggagtaaat accacagcca gctgatatta aaatgcgacc agccgtacgc cccgaggatc
     7741 tcatcactcg tggactccag caggccataa tcgaggctcc agctcggcaa caggacaaat
     7801 gccagccaac ccagcaaaac ccaaaggaaa atcgcatccc gctttttcac gggatggagt
     7861 gcaagtgtct gtgacatagc agttccggta tggttggccg gggcaggcgt ctgcgctgca
     7921 cccggcaaat gcgcctaatg cgggtttatt tacccatttt tacttcgcta acccatttat
     7981 taatcaatgc cttacgaaca tccgtggaac cgtatttgtc catgtcgtag ttgattaatt
     8041 tcaggtcgtc gagcttcagt gagtttggcg acgtttcggc ggtggtattg gtcaggatct
     8101 gataagactt ccctttcttc cacgccagtt cctgcgcttc ttttgataat acccagtcca
     8161 caaacagttt ggcgttgtcg aggttgcgcg cgcctttcag aatgctgacg ccgccaattt
     8221 cgtagcctgt accttcacac ggggaaatca actccagcgg cgcgccctgt tctttttcca
     8281 gcgagtagtc atgcaggaag ccaataccaa tcgccgtttc gccacgggcg gcgttacgtg
     8341 ccggggctat gcctgatttg gtgtactgag agacgttggc gttcagttgt tttagataat
     8401 caaaggcctg atcttccccc cacagctgcg caaaagtagc cagggcggtg taggcagtac
     8461 cggaactttg cggatcggca atttgaattt cgcctttata ttccggtttg gtgagatctt
     8521 tccagcactt cggcaccggc aggtttttct ctttcagacg atcggtgttt acgccaaaac
     8581 cgaggatccc cacataaacg gcagaggaca gattcccttt cacttttgcc ggatcgcgga
     8641 atttctccat gatctgctca aggttagggg atttatacgc ctgtagcagg cccatctcgc
     8701 cagcctgcga ttgcgggtct aaagtgccgc cgtaccagac gtcagcctgg gggtttttct
     8761 tttcagcatc gacttttgcc agggtgcttc cggaaccgtt gcggataaac gaggttttta
     8821 catcatattt ctcgccaaac gctttggttt cggcctcaca catttcattg gtggcgctgc
     8881 aatagaccac cagccggcct tttgcctgcg cggcacccgt taaagtcgcc agcgcaacgg
     8941 cagaagcaat cagagtagag gtaaaggtca ttttcattgt tttatccttc gagcgtgagt
     9001 ggtggtgatg cccgccatca gtaacggcat cagcagtaaa cccataagta ctgcggcgac
     9061 cgagagtaaa ctgaacattc ccggccagcc gtagcgttcg atgaccagcg acagcggcca
     9121 gcctgccagc gccgcgccaa ggtaagcgaa caggccgaga aatccggtga ttgaacccgc
     9181 cgcagctttg tgcccacact ccaccgccgc aagtccaatc agcatttgtg ggccaaagac
     9241 gaaaaagccc acggtaaaaa agcacactgc cagcagagcg tagtgatgaa ccggtgccag
     9301 ccacagtgcg gcgactgaca ccatcaaccc caacgtgaac agcaaaatca ttggcgctcg
     9361 ttgcccgcta aacagcaaat ccgatcccca tccggcaaat aacgcgccga gtaagccgcc
     9421 cacttcaaac agcattaccg tggcgttggc gctgagcagg ttgacaccgt ggctttccgt
     9481 cagccagata ttgccccagt cgttaagcgc aatccggatc acataaacca gtacataaga
     9541 gacgcccagt agccagatca gcggattttg cagcatggtg gtgcgcaaca tttgccacag
     9601 ccccatcggc ggactttgtt gttcctgacg taactccagc ggatcgtgac gccagtgacc
     9661 aactgtcggt aatccctctt cctggggcgt gcctttgagt tgtagcgtta accagatccc
     9721 taatgccatg ctgattatcc ccggtgtcag catcgcggcc tgccagcccc accagtgggc
     9781 ggcaaaggcg ctaatcagcg gaataatcgc tccgccaatg ttgatcgaca tattccagca
     9841 gccccaccag aaaccgcgct cattgcgtga gtaccagtgg gtcagcaagc gggcacacgg
     9901 tggccatccc cagccctgaa aaaaaccgtt cagtgtccag accacaagca gaagcgtaag
     9961 tgattcgcca aaggcaaaca ccacgttcaa taacccggtg gcgaacaagc cgacgcccat
    10021 aaaaccgcgt tgtccgtggc tgtcatgcca cagtccggcg gtaaatttcg acaatccgta
    10081 gctgagataa aacagcgaac ccagcaggcc aatgtcgcct ttatccagtc cgaggtcggt
    10141 ttgtagcgcc ggaaggacgt aattcacgct ttttcgcgtc agataaaatg cggcgtagcc
    10201 aataatcatg cacatcagca atcgcggacg taatgtccgg tagcattgat taatttctct
    10261 cgctgaacgc gcgtgcatgg ttgtctccat gttgctgact atatgaaaat gaaagtgaaa
    10321 ggggatgaga gaaaatccgg gatacctaag actttttcct ggttatccgg cgatttgttg
    10381 caaaaatgtg ggcaagttaa caattacccg cgtgccgtgc tgtttttcga gcgttaagtc
    10441 gccgcccagg gcgctgacgc gctcatgcat cccctgaatg ccaaatccgg ccattttccc
    10501 gctggcgatc cccacaccgt tgtcgctgac ttccagatgc aatacctctc cctgctggcg
    10561 caggataatc gtcacttcgc tggcttcggc gtgtttacag atgttattaa gcaactcctg
    10621 caataaccga tacaaggtaa agcgcaccgt ttcgttttct ggtgtggagt tcagttgata
    10681 ggcaaactgg caatgaatgc cgcgttcact aaaggcaaat tcattaatca gatgcaacaa
    10741 cgcttcgcga aatgtcagtt catcaagcgc gggaggccgc aactggcgca gcaattggcg
    10801 ggtggagaga tgaatgcggc gggcgagctc attgatttgg ctggctgcgg attgaatttg
    10861 cgccggatca cgcgcccgct tcaccagttg cgactggatc tgaatggcgg tgatgttctg
    10921 accgatttcg tcatgcagct ctcgcgccag gttttttcgc gtgtcctcct cggtgtggat
    10981 cagcttttcc gccaatgccc gccgcgctgc cagctcggcc tccagccgtt gtcggtaatg
    11041 gtgcaggttt agcgccagat gttgctggcg actgatggca atgcccaggc caattcccag
    11101 caaggcctgg gtcgcgagga aaatctccag ctccaccagg ttactaaagc ccacgccaat
    11161 ctggcgggcg atggtgatca tcatgctgcc caaaagcccg gagagtacgc caccttgcca
    11221 gccaaatttc cacgccatca ccacattggg caaaaagacc acgatcaaca gcagacgttc
    11281 aatttcaggt gacagcacta tctgagtgcc gataccaata ataaaaaaca ggctacacca
    11341 gatgattaaa gaggtacgca aggggggatt atgcgtatcg agtcccagga gctggtagcg
    11401 atgctgctgg cgcagaaact caaagataag atagacaaac ggcgtcagta atacgccgcc
    11461 ggtaaatgag gtcaaacccg gcagcattat tgggcctgac ataaaagggg agagcagggc
    11521 ggtattcaac aatgaagctg ccgtgacagt ggctaacagc aacgtcagac gctgccagta
    11581 aagtggaaaa cggtgccaga agttttgcgc aaggagggca ggaaacaggc taataaccgg
    11641 cgttgccagc atgagataac cgttcaatag ctgttcacta tggagccaga acatcatcag
    11701 caaagggggg agcaccaacg caggccagta gcggcgagag agcaaaataa caagggcgag
    11761 ataaactccc tgcgggagaa acaacaccgc ctgttgaccg ttattggtca gataaaagct
    11821 gagcgtccac agcatcagcc agccggttcc ccaggccagc acaataaaca gcgaaatcac
    11881 ccaatgacga agattgcgcg acattaatgc cccgccagca gatggtggtc gagcgcaaaa
    11941 tgtaccagtt caatggtgct atggcagttc agttttccca gcacattggc gcgatgcaca
    12001 tgtaccgttt tatgactgag ttcgagttta aaggcgatct ctttaacgct gtctcctttg
    12061 acaagtaaat caaacacttc acgttctcgt ggggtgagaa tttccagcgc ctgggcgggt
    12121 tgcccgccgc cgcgtaaagc ccgaatcgca tcggcacaga gataatgccc gcccagcccg
    12181 accgagcgca ccgcctgtac taactcttcc ggcccacagc gtttggttaa atacccgctg
    12241 gcaccggcat ccagcgcgct ttgcacaaag gttggcgcgt cataaatgct aagaataatg
    12301 gcgcgaaatt gcggtttttg tacacgcagg cgcttaagca aactgaggcc gttttcgtcc
    12361 ggcatggcaa tatcaatgac ggcaacattg acgtcatcac ggattaatgc cgaccatgcc
    12421 tgcgcggcac tactgtactg accaatgacc tccagatcgt cttccagaga gagtaattgt
    12481 gcgaagccag agcgcaccac cacatggtca tccaccagca ctacccgaat catacttttc
    12541 ttctcttatt atctaattct cctatcatgc atcacggcgc ggcgctggca atcaaccagc
    12601 gacagatatg gaatggatgt aacaaaatgg caataatttg ccggagcgcg cacatcttat
    12661 ctggcttgtg aaccggcggg atacgttcgc caaaaccgca aaatgcgcaa actcgctaac
    12721 cattgccgat ttttttctaa aaacgctttg ttcttaacaa gaccttttaa ttcctttatc
    12781 aaactgaccc gcaacgaaat actgctccca taacaagaca ggggagcaga caatcatggc
    12841 aatttcatcg cgtaacacac ttcttgccgc actggcattc atcgcttttc aggcacaggc
    12901 ggtgaacgtc accgtggcgt atcaaacctc agccgaaccg gcgaaagtgg ctcaggccga
    12961 caacaccttt gctaaagaaa gcggagcaac cgtggactgg cgtaagtttg acagcggagc
    13021 cagcatcgtg cgggcgctgg cttcaggcga cgtgcaaatc ggcaacctcg gttccagccc
    13081 attagcggtt gcagccagcc aacaggtgcc gattgaagtc ttcttgctag cgtcaaaact
    13141 gggtaactcc gaagcactgg tggtaaagaa aactatcagc aaaccggaag atctgattgg
    13201 caaacgcatc gccgtaccgt ttatctccac tacccactac agcctgctgg cggcgctgaa
    13261 gcactggggt attaaacctg ggcaagtgga gatagttaac ctgcagccgc ccgcgattat
    13321 cgctgcatgg cagcggggag acattgatgg tgcttatgtc tgggcaccgg cggttaacgc
    13381 cctggaaaaa gacggcaagg tgttgaccga ttctgaacag gtcgggcagt ggggcgcgcc
    13441 aacgctggac gtctgggtgg tgcgcaaaga ttttgccgag aaacatcctg aggtcgtgaa
    13501 agcgttcgct aaaagcgcca tcgatgctca gcaaccgtac attgctaacc cagacgtgtg
    13561 gctgaaacag ccggaaaaca tcagcaaact ggcgcgttta agcggcgtgc ctgaaggtga
    13621 cgttccgggg ctggtgaagg ggaataccta tctgacgccg cagcaacaaa cggcagaact
    13681 gaccggaccg gtgaacaaag cgatcatcga caccgcgcag tttttgaaag agcagggcaa
    13741 ggtcccggct gtagcgaatg attacagcca gtacgttacc tcgcgcttcg tgcaataaaa
    13801 ggaggcgcag atgctgcaaa tctctcatct ttacgccgat tatggcggca aaccggcact
    13861 ggaagatatc aacctgacgc tggaaagcgg cgagctactg gtggtgctgg ggccgtccgg
    13921 ctgcggtaaa accaccctgc tgaatctgat tgccggtttt gtgccttatc agcatggcag
    13981 cattcaactg gcgggtaagc gtattgaggg accgggagca gagcgtggcg tagtttttca
    14041 gaatgaaggg ctactaccgt ggcgcaatgt acaggacaac gtggcgttcg gcctgcaatt
    14101 ggcaggtata gagaaaatgc agcgactgga aatcgcgcac cagatgctga aaaaagtggg
    14161 gctggaaggc gcagaaaaac gctacatctg gcagctttcc ggtggtcaac gtcagcgggt
    14221 gggcattgcc cgcgcgctgg cggcgaatcc ccagctgtta ttactcgacg aaccgtttgg
    14281 tgcgctggac gccttcaccc gcgaccagat gcaaaccctg ctgctgaaac tctggcagga
    14341 gacgggcaag caggtgctgt tgattaccca cgatatagaa gaagcggtgt ttatggcgac
    14401 tgaactggtt ctgctttcat ccggccctgg acgtgtgctg gagcggctgc cgctcaactt
    14461 tgctcgccgc tttgttgcgg gagagtcgag ccgcagcatc aagtccgatc cacaattcat
    14521 cgccatgcgc gaatatgttt taagccgcgt atttgagcaa cgggaggcgt tctcatgagt
    14581 gtgctcatta atgaaaaact gcattcgcgg cggttgaaat ggcgctggcc gctctcgcgt
    14641 caggtgacct taagcattgg cacgttagcg gttttactca ctgtatggtg gacggtggcg
    14701 gcgctgcaac tgattagccc gctgtttttg ccgccgccgc aacaagtact ggcaaaactg
    14761 ctcaccattg ccggaccgca gggatttatg gacgccacgc tgtggcagca tctggcagcc
    14821 agcctgacgc gcattgtgct ggcgctactc gcggcggtgt tgatcggcat tccggtcggg
    14881 atcgccatgg ggctaagccc gacggtgcgc ggcattctgg atccggtaat cgaactttat
    14941 cgtccggtgc cgccgctggc ttatttacca ctgatggtga tctggtttgg tattggcgaa
    15001 acctcgaaga tcttactgat ctatttagcg atttttgcac cggtggcgat gtcggcgctg
    15061 gcgggggtga aaagcgcgca gcaggttcgc attcgtgccg cccagtcgct gggtgccagc
    15121 cgtgcgcagg tgctgtggtt tgtcattttg cccggcgcgc tgccggaaat cctcaccgga
    15181 ttacgcattg gtctgggggt gggctggtct accctggtag cggcggagct gattgccgcg
    15241 acgcgcggtt taggatttat ggttcagtca gcgggtgaat ttctcgcgac tgacgtggtg
    15301 ctggcgggga tcgcggtgat tgcgattatc gcctttcttt tagaactggg tttgcgcgcg
    15361 ttacagcgcc gactgacgcc ctggcatgga gaagtacaat gagtgaacgt ctgagcatta
    15421 ccccgctggg gccgtatatc ggagcacaaa tttcgggtgc cgacctgacg cgcccgttaa
    15481 gcgataatca gtttgaacag ctttaccatg cggtgctgcg ccatcaggtg gtgtttctgc
    15541 gcgatcaagc tattacgccg ctgcagcaac gcgcgctggc ccagcgtttt ggcgaactgc
    15601 atattcaccc tgtttaccct catgccgaag gggttgacga gatcatcgtg ctggataccc
    15661 ataacgataa tccgccagat aacgacaact ggcataccga tgtgacattt attgaaacgc
    15721 cacccgcagg ggcgattctg gcagcgaaag agttaccttc gaccggcggc gatacgctct
    15781 ggaccagcgg tattgcggcc tatgaggcgc tctctgttcc cttccgccag ctgctgagcg
    15841 ggctgcgtgc ggagcatgat tttcgtaaat cgttcccgga atacaaatac cgcaaaaccg
    15901 aggaggaaca tcaacgctgg cgcgaggcgg tcgcgaaaaa tccgccgttg ctacatccgg
    15961 tggtgcgaac gcatccggtg agcggtaaac aggcgctgtt tgtgaatgaa ggctttacta
    16021 cgcgaattgt tgatgtgagc gagaaagaga gcgaagcctt gttaagtttt ttgtttgccc
    16081 atatcaccaa accggagttt caggtgcgct ggcgctggca accaaatgat attgcgattt
    16141 gggataaccg cgtgacccag cactatgcca atgccgatta cctgccacag cgacggatta
    16201 tgcatcgggc gacgattctt ggggataaac cgttttatcg ggcggggtaa tacgagagtg
    16261 gacggtcccc tcgccccctt ggggagaggg ttagggtgag ggggcgttca ccgtactttc
    16321 aacaggttaa ctcccccttt ctgagaggaa acaaaattaa cgcagaatct tcttctcagc
    16381 caaatccagc gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa tcgaacctaa
    16441 gctttcgagc accactttct cttcatctat agcacccgcc agcgcggcga acttaatcat
    16501 cgcgtactca ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc
    16561 acgcacgatg tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc
    16621 ttcatccagc agtgattcac gaatcgcctc gcgacggttc attgggttca tctgatagct
    16681 tttgcggtcg ccttttaatg cgcttccggc agcttcacgg aacggaccat aaaaggagga
    16741 ggcgaacttg gtcgaatacg acataatcgc cgtatcttta aagccagcag cgtccagcgc
    16801 ctggcgaatc gcctgtacct ggccatccat cgcggcggaa ggggcgatga agtctgcacc
    16861 tgcagcagct gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac
    16921 gccatgctcg cacagcacac cgcagtgacc gtgagaagtg tattcacaga agcaggtgtc
    16981 tgacatgaca atcatttctg gcactgtctg cttgcagatg cgcgacatac gcgccaccag
    17041 tccatcttcc cgccaggcat cgctgccggt ttcatcggta tggtgagaga tgccaaaagt
    17101 catcacggaa cgaataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc
    17161 tggaatgcgc atcacgcctg gcatggcttc aacggctttg tagtcgtcaa tttcttcttc
    17221 aacaaagatc ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg
    17281 cagcgcagga gatttgcgca ggcgacgagg gcgttggatt aaatctgtca tggtctgcct
    17341 gatgtttgtg gaatcgaggg gcatagtata cctgaagcag ggtagggatg ttttacgaaa
    17401 gttgtcgcga tgttgacaag aagagaatgg aagagaggcc aggacatctt tgtaata
//
LOCUS       DAZKFU020000085        17318 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZKFU020000085 DAZKFU020000000
VERSION     DAZKFU020000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.086"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1336)
                     /gene="djlB"
                     /locus_tag="ACHOQQ_RS11350"
                     /old_locus_tag="pgaptmp_002270"
     CDS             complement(<1..1336)
                     /gene="djlB"
                     /locus_tag="ACHOQQ_RS11350"
                     /old_locus_tag="pgaptmp_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGU4470511.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEECEVAEHEILLAFSALLASDSERFLPSAWQRFIQQLNY
                     CSMDEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENTGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGKSLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDD"
     gene            complement(1333..2040)
                     /gene="ybeR"
                     /locus_tag="ACHOQQ_RS11355"
                     /old_locus_tag="pgaptmp_002271"
     CDS             complement(1333..2040)
                     /gene="ybeR"
                     /locus_tag="ACHOQQ_RS11355"
                     /old_locus_tag="pgaptmp_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4470512.1"
                     /translation="MDMKSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            2204..3181
                     /gene="ybeQ"
                     /locus_tag="ACHOQQ_RS11360"
                     /old_locus_tag="pgaptmp_002272"
     CDS             2204..3181
                     /gene="ybeQ"
                     /locus_tag="ACHOQQ_RS11360"
                     /old_locus_tag="pgaptmp_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HGU4470513.1"
                     /translation="MIFTSSCCDNLSIDKIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVAWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3251..3733)
                     /gene="ybeL"
                     /locus_tag="ACHOQQ_RS11365"
                     /old_locus_tag="pgaptmp_002273"
     CDS             complement(3251..3733)
                     /gene="ybeL"
                     /locus_tag="ACHOQQ_RS11365"
                     /old_locus_tag="pgaptmp_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGU4470514.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            3969..6551
                     /gene="leuS"
                     /locus_tag="ACHOQQ_RS11370"
                     /old_locus_tag="pgaptmp_002274"
     CDS             3969..6551
                     /gene="leuS"
                     /locus_tag="ACHOQQ_RS11370"
                     /old_locus_tag="pgaptmp_002274"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGU4470515.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDSEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6566..7147
                     /gene="lptE"
                     /locus_tag="ACHOQQ_RS11375"
                     /old_locus_tag="pgaptmp_002275"
     CDS             6566..7147
                     /gene="lptE"
                     /locus_tag="ACHOQQ_RS11375"
                     /old_locus_tag="pgaptmp_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGU4470516.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7147..8178
                     /gene="holA"
                     /locus_tag="ACHOQQ_RS11380"
                     /old_locus_tag="pgaptmp_002276"
     CDS             7147..8178
                     /gene="holA"
                     /locus_tag="ACHOQQ_RS11380"
                     /old_locus_tag="pgaptmp_002276"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGU4470517.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8180..8821
                     /gene="nadD"
                     /locus_tag="ACHOQQ_RS11385"
                     /old_locus_tag="pgaptmp_002277"
     CDS             8180..8821
                     /gene="nadD"
                     /locus_tag="ACHOQQ_RS11385"
                     /old_locus_tag="pgaptmp_002277"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGU4470518.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            8845..9456
                     /gene="cobC"
                     /locus_tag="ACHOQQ_RS11390"
                     /old_locus_tag="pgaptmp_002278"
     CDS             8845..9456
                     /gene="cobC"
                     /locus_tag="ACHOQQ_RS11390"
                     /old_locus_tag="pgaptmp_002278"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGU4470519.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9716..10033
                     /gene="rsfS"
                     /locus_tag="ACHOQQ_RS11395"
                     /old_locus_tag="pgaptmp_002279"
     CDS             9716..10033
                     /gene="rsfS"
                     /locus_tag="ACHOQQ_RS11395"
                     /old_locus_tag="pgaptmp_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGU4470520.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10037..10504
                     /gene="rlmH"
                     /locus_tag="ACHOQQ_RS11400"
                     /old_locus_tag="pgaptmp_002280"
     CDS             10037..10504
                     /gene="rlmH"
                     /locus_tag="ACHOQQ_RS11400"
                     /old_locus_tag="pgaptmp_002280"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGU4470521.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10535..12436
                     /gene="mrdA"
                     /locus_tag="ACHOQQ_RS11405"
                     /old_locus_tag="pgaptmp_002281"
     CDS             10535..12436
                     /gene="mrdA"
                     /locus_tag="ACHOQQ_RS11405"
                     /old_locus_tag="pgaptmp_002281"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGU4470522.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12439..13551
                     /gene="mrdB"
                     /locus_tag="ACHOQQ_RS11410"
                     /old_locus_tag="pgaptmp_002282"
                     /gene_synonym="rodA"
     CDS             12439..13551
                     /gene="mrdB"
                     /locus_tag="ACHOQQ_RS11410"
                     /old_locus_tag="pgaptmp_002282"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGU4470523.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13562..14650
                     /gene="rlpA"
                     /locus_tag="ACHOQQ_RS11415"
                     /old_locus_tag="pgaptmp_002283"
     CDS             13562..14650
                     /gene="rlpA"
                     /locus_tag="ACHOQQ_RS11415"
                     /old_locus_tag="pgaptmp_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGU4470524.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLSATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLRQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14790..16001
                     /gene="dacA"
                     /locus_tag="ACHOQQ_RS11420"
                     /old_locus_tag="pgaptmp_002284"
     CDS             14790..16001
                     /gene="dacA"
                     /locus_tag="ACHOQQ_RS11420"
                     /old_locus_tag="pgaptmp_002284"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGU4470525.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16112..16375
                     /gene="ybeD"
                     /locus_tag="ACHOQQ_RS11425"
                     /old_locus_tag="pgaptmp_002285"
     CDS             16112..16375
                     /gene="ybeD"
                     /locus_tag="ACHOQQ_RS11425"
                     /old_locus_tag="pgaptmp_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGU4470526.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16476..17117
                     /gene="lipB"
                     /locus_tag="ACHOQQ_RS11430"
                     /old_locus_tag="pgaptmp_002286"
     CDS             16476..17117
                     /gene="lipB"
                     /locus_tag="ACHOQQ_RS11430"
                     /old_locus_tag="pgaptmp_002286"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGU4470527.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4352 a         4368 c         4432 g         4166 t
ORIGIN      
        1 catcgtcaac gggcattggc tgcggttgcg gctccggttc aacagtttgc ttctcggtaa
       61 aattcgccgc ctgaattaat aatcgacgaa taattaatgc ttccagtgga caacgatttt
      121 ctgcatcaag acggcttacg atcggcatta atgattcttc tgttggttgg ctggcctgca
      181 ctaattgttt ggcaactaaa tataatggcc ataagggatg ttctgtatca cgcgtgagtg
      241 agatccgcgg cttacgtcct ttgtgcaaaa ccatctcgac cagagaacgc cccagcgcag
      301 agagtttttc atcactgagt atttcgtata aatgaaaacg cgtacgagcg ctgtcaccag
      361 ggacataaag tagcggtttt ccctgatcgt taaccagatc acgcgcttca atcatcatca
      421 ccactaatgg gtaataatcg acccgatgct gacggcacca ttgcaacatt aaagcatccg
      481 cctgggtgga gggatattcg cgccagtagt cacaaagttc tgccagcagg cttttgcctt
      541 ctccacaata gacgcgttgc gcgtattcat aataacgcgc atcttcatta tcaggttcag
      601 cttcccgcca cgaccgggcg taatccagga gttcgggaat tcccaggcga cctgcgctat
      661 accagcggag taaatttctg tgcagttttg catcatcggg gataatcact gcgcgatgtt
      721 gcgctaaata caatgtcagc cattctggat gcgatgacca aatccgagcg aacatttggt
      781 aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg cagaatggtc tggaagttaa
      841 aaacattccc cttcgcaatg gcatataaaa aggattccag ttcttcttcg tctatttccc
      901 cggtattttc ctcctgtaac caccgcaatc tttctgctaa taacaccacg cactcgaagg
      961 ataaatgggc agtgttcatg gctattgtgc acagcgacca gcgtaattca tcaatctcat
     1021 ccatcgagca ataatttaat tgctgaatga atcgctgcca ggcggagggc agaaaacgtt
     1081 cactatcaga ggcaagtaac gcactaaagg cgagcagaat ttcatgttct gctacctcac
     1141 attcttctgg ttgccaaaca gatttagcag gcgactgcgc aatccggagc gcttcctcat
     1201 acgcctgacg aagttgttta aaaccctgcg gatcggtttc tggatgaaag gacggtaaca
     1261 gcgccagata agcgcggcgg ataatatcga cgtcagtcgt ttcctctatg ttgaggatct
     1321 tccagcaatt tttcataggt cgctcatatc cttaagcgtt tccgggcatg tggggtagtc
     1381 agccagtggt tgccagggta aggtcgcgta atatgcatcg tcactggcaa ataattgata
     1441 aaacatatca ttgccggaat cgtcgtattc tttacgcgct aagaattcac gtaataaagg
     1501 cagattatca tcttcgggag actgccaata ttgccgacca aaggtataac cggcaaagta
     1561 ttgccgccag ttactataat aacgtagagc ccgtagatga atacgcgact ggatccatag
     1621 actttcttct tcactcaacc agttattaag tacgcccatt cgacttaaaa aacccatccg
     1681 gacataatcc catgctttaa ttcctccctc accacagcat tcggctgtgc tggcgacaaa
     1741 ttgcgcataa gcctgacctt gctcatttaa ttcagcaaga tagtcacgcc attcacatgg
     1801 agaataacga aaccagcggt gataaaaccc tgccaaccga gcggcatgac cgttatcgat
     1861 cattcggtga attgtgcgca gcaggtcatc acggtcatca attccccagg aggaagaaag
     1921 gtcaaaaaag catgtaccta aatacatttt gggggatgaa tgcgaaggta aacagcattc
     1981 attgcgtatt tccatcggag tggaaagtgc aaacaatatt ttttgcgact tcatgtccat
     2041 ataatttatc cttaaacatt catttagtga atgtaaacta cattacgagg ctcatttatt
     2101 tatacttttc tgacacaaca taatacgcat aaaataagac ttacgcaaaa acggaacaca
     2161 taaaaatata atatgtttta tctgcaacca taaggaaatg ataatgattt ttacgtcaag
     2221 ttgctgcgat aatttatcaa tagataagat tatcgaacgt gctgaaaaag gcgattgcga
     2281 ggctcaatat attgttgggt tttattataa tcgcgatagc gcaattgact ctccggacga
     2341 cgaaaaggcc ttttactggc tgaagctggc cgctgagcaa ggtcattgtg aagcacaata
     2401 ctccttaggg caaaagtata ccgaggataa aagccgtcat aaagataatg agcaagccat
     2461 cttctggctg aaaaaagctg ccctacaagg ccatactttc gcttccaacg cccttggctg
     2521 gatactggat cgtggagaag accccaacta taaagaagcg gttgcctggt atcagatagc
     2581 cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg tggatgtaca gaaatggcaa
     2641 cggagtcgca caagactata cgctggcatt tttctggtac aaacaagctg cattacaagg
     2701 ccatagtgac gcgcaaaaca atctggccga tctttatgaa gacggaaaag gcgttgctca
     2761 aaacgagaca ctcgccgcat tctggtattt gaaaagcgca cagcagggta atcggcatgc
     2821 tcagtttcaa attgcatggg attataacgc tggcgaaggg gtggaccagg actataagca
     2881 agcgatgtac tggtatctga aggctgccgc tcaggggagc gtcggcgctt acgtcaacat
     2941 cggttatatg tataaacacg gacaaggcgt tgagaaagat tatcaggctg cctttgaatg
     3001 gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg tataacctgg ccattatgta
     3061 tcactacgga gaaggaagac ctgtcgatct ccgacaggct ctcgacctgt atcgtaaagt
     3121 tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt gagactgaag atttactgta
     3181 gagataaaaa caagggccgg ggaatttcat tcggcccata ttctctttat tgctgtgcga
     3241 aatcagacta ttacggctca aacgggcgtc tctggaactg gtcatgacca catttcgggc
     3301 atagcgtcag cacttccggt gtgtagatcg ggagatggaa gtgacatttc tcgcagacca
     3361 gatttcccag cccgaccact tctccgctgt gataaacccc atgatgattg aggtcctgga
     3421 aaacttcgcg ccattcaagc tgcgttttat cggtgatgtc tgccagctcc tgccacaagc
     3481 tttctttaat cacccgcata aagacgctgt cagattcttc tttcaggctc tcttcatagc
     3541 tcatggcgaa ctcttccagg tcacgtctga cagctcgcgt cagctcatcg acctcggttc
     3601 gcgttaactc ccctgttttt attacgcgct cgcgcgcctg ttccaccagt gcgtcgatat
     3661 cacgttcgcc attgcgcagg cgttcgctca gtgacgcaac cagttcacgg taatattgag
     3721 caaccttgtt catcgtttca tctcccgggt cgttaactga ctataataat agacgttctt
     3781 tgttccttgc tttgctaata cgcccacaaa ctctgtaaat taatgtatga ataatccggg
     3841 catcatttca acgtcgagtg cgcgaaaggc tgttttgacg cggacgtttg ggctatgcta
     3901 tgcggatctg aaaaaccacc tctaacgcta cttttgtagc cgtaatgaaa acaggaccac
     3961 tggctgccat gcaagagcaa taccgcccgg aagagataga atccaaagta cagcttcatt
     4021 gggatgagaa gcgcacattt gaagtaaccg aagacgagag caaagagaag tattactgcc
     4081 tgtctatgct tccctatcct tctggtcgac tacacatggg ccacgtacgt aactacacca
     4141 tcggtgacgt gatcgcccgc taccagcgta tgctgggcaa aaacgtcctg cagccgatcg
     4201 gctgggacgc gtttggtctg cctgcggaag gcgcggcggt gaaaaacaac accgctccgg
     4261 caccgtggac gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg ctgggctttg
     4321 gttatgactg gagccgcgag ctggcaacct gtacgccgga atactaccgt tgggaacaga
     4381 aattcttcac cgagctgtat aaaaaaggcc tggtatataa gaagacttct gcggtcaact
     4441 ggtgcccgaa cgaccagacc gtactggcga acgaacaagt tatcgacggc tgctgctggc
     4501 gctgcgatac caaagttgaa cgtaaagaga tcccgcagtg gtttatcaaa atcactgctt
     4561 acgctgacga gctgctcaac gatctggata aactggatca ctggccagac accgttaaaa
     4621 ccatgcagcg taactggatc ggtcgttccg aaggcgtgga gatcaccttc aacgttaacg
     4681 actatgacaa cacgctgacc gtttacacta cccgcccgga cacctttatg ggttgtacct
     4741 acctggcggt agctgcgggt catccgctgg cgcagaaagc ggcggaaaat aatcctgaac
     4801 tggcggcctt tattgacgaa tgccgtaaca ccaaagttgc cgaagctgaa atggcgacga
     4861 tggagaaaaa aggcgtcgat actggcttta aagcggttca cccattaacg ggcgaagaaa
     4921 ttcccgtttg ggcagcaaac ttcgtattga tggagtacgg cacgggcgca gttatggcgg
     4981 ttccggggca cgaccagcgt gactacgagt ttgcctctaa atacggcctg aacatcaaac
     5041 cggttatcct ggcagctgac ggctctgagc cagatctttc tcagcaagcc ctgactgaaa
     5101 aaggcgtgct gttcaactct ggcgagttca acggtcttga ccatgaagcg gccttcaacg
     5161 ccatcgccga taaactgact gcgatgggcg ttggcgagcg taaagtgaac taccgcctgc
     5221 gcgactgggg ggtttcccgt cagcgttact ggggcgcgcc gattccgatg gtgacgctgg
     5281 aagacggtac cgtaatgccg accccggacg accagctgcc ggtgatcctg ccggaagatg
     5341 tggtaatgga cggcattacc agcccgatta aagcagatcc ggagtgggcg aaaactaccg
     5401 ttaacggtat gccagcactg cgtgaaaccg acactttcga cacctttatg gagtcctcct
     5461 ggtactatgc acgctacact tgcccgcagt acaaagaagg tatgctggat tccgaagcgg
     5521 ctaactactg gctgccggtg gatatctaca ttggtggtat cgaacacgcc attatgcact
     5581 tgctctactt ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg aactctgacg
     5641 aaccagcgaa acagttgctg tgtcagggta tggtgctggc agatgccttc tactatgttg
     5701 gcgaaaacgg cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa cgtgacgaga
     5761 aaggccgtat cgtgaaagcg aaagatgcgg caggccatga actggtttat accggcatga
     5821 gcaaaatgtc caagtcgaag aacaacggta tcgacccgca ggtgatggtt gaacgttacg
     5881 gcgcggacac cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg actctcgaat
     5941 ggcaggaatc cggcgtggaa ggggctaacc gcttcctgaa acgtgtctgg aaactggtgt
     6001 acgagcatac tgcgaaaggt gatgttgcgg cactgaacgt tgatgcgctg actgaagatc
     6061 agaaagcgct gcgtcgcgat gtgcataaaa cgatcgctaa agtgaccgat gatatcggcc
     6121 gtcgtcagac cttcaacacc gcaattgcgg cgattatgga gctgatgaac aaactggcga
     6181 aagcaccgac cgatagcgag caggatcgcg ccctgatgca ggaagcgctg ctggccgttg
     6241 tccgtatgct taacccgttc accccgcaca tctgcttcac gctgtggcag gaactgaaag
     6301 gcgaaggcga tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg atggtggaag
     6361 actccacgct ggtcgtggtg caggttaacg gtaaagtccg tgccaaaatc accgttccgg
     6421 tggacgcaac ggaagaacag gttcgcgaac gtgctggcca ggaacatctg gtagcaaaat
     6481 atcttgatgg cgttactgta cgtaaagtga tttacgtacc aggtaaactc ctcaatctgg
     6541 tcgttggcta agcgcgggag gaagcgtgcg atatctggca acattgttgt tatctctggc
     6601 ggtgttaatc accgccgggt gtggctggca tctgcgtgat accacgcagg ttccttccac
     6661 tatgaaggtc atgatcctgg actcaggtga tccgaacggg ccattaagcc gtgcggtgcg
     6721 taaccagtta cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc gtaaggacgt
     6781 tccatccctg cgtttaggta aagtgagcat cgcgaaagat accgcatcgg tattccgtaa
     6841 cggtcaaaca gcagagtatc agatgatcat gacggtgaat gcgaccgtgt tgatccccgg
     6901 ccgtgacatc tacccgatta gcgccaaggt cttccgttcg ttcttcgata acccgcaaat
     6961 ggcgttagcg aaagataacg aacaagacat gatcgtaaaa gagatgtacg accgtgctgc
     7021 cgaacagctg attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt ccgacgaaga
     7081 acagacgtcg actacaacgg atactccggc aacgcctgca cgcgtctcca ccacgctggg
     7141 taactgatga ttcggttgta cccggaacaa ctccgcgcgc agctcaatga agggctgcgc
     7201 gcggcgtatc ttttacttgg taacgatcct ctgttattgc aggaaagcca ggacgctgtt
     7261 cgtcaggtag ctgcggcaca aggattcgaa gaacaccaca ctttttccat tgatcccaac
     7321 actgactgga atgcgatctt ttcgttatgc caggccatga gtctgtttgc cagtcgacaa
     7381 acgctattgc tgttgttacc agaaaacgga ccgaacgcgg cgatcaatga acaacttctc
     7441 acactcaccg gacttctgca tgacgacctg ctgttgatcg tccgcggtaa taaattaagc
     7501 aaagcgcaag aaaatgccgc ctggtttact gctcttgcga atcgcagcgt gcaggtgacc
     7561 tgtcagacac cggagcaggc tcagcttccc cgctgggttg ctgcgcgcgc aaaacagctc
     7621 aacttagaac tggatgacgc ggcgaatcag gtgctctgct actgttatga aggtaacctg
     7681 ctggcgctgg cccaggcact ggagcgttta tcgctgctct ggccagacgg caaactgaca
     7741 ttaccgcgcg ttgaacaggc ggtgaatgat gccgcgcatt tcaccccttt tcattgggtt
     7801 gatgctttgt tgatgggaaa aagtaagcgc gcgttgcata ttcttcagca actgcgtctg
     7861 gaaggcagcg agccggttat tttgttgcgc acattacaac gtgaactgtt gttactggtt
     7921 aacctgaaac gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa gcatcgggta
     7981 tggcagaacc gccggggcat gatgggcgag gcgttaaatc gcttaagtca gccgcagtta
     8041 cgtcaggccg tgcaactcct gacacgaacg gaactcaccc tcaaacaaga ttacggtcag
     8101 tcagtgtggg cagagctgga agggttatct cttctgttgt gccataaacc cctggcggac
     8161 gtatttatcg acggttgata tgaaatcttt acaggctctg tttggcggca cctttgatcc
     8221 ggtgcactat ggtcatctaa aacccgtgga aacgctggcg aatttgattg gtctgacgcg
     8281 agtcacaatc atccctaata atgttcctcc gcatcgtccc cagccggaag cgaacagcgt
     8341 acagcgtaaa cacatgcttg aactggcgat tgccgacaag ccattattta ctcttgatga
     8401 acgcgagcta aagcgcaatg ccccctctta cactgcgcaa acactgaaag agtggcggca
     8461 ggaacaaggg ccggacgtgc cgctggcgtt tattattggt caggattcat tgctgacctt
     8521 tccgacctgg tacgaatacg aaacgatact cgacaatgca catttgatcg tctgtcggcg
     8581 tccaggttac ccacttgaaa tggcgcaacc gcaataccag caatggctgg aagatcattt
     8641 gacacataac ccggaagatc ttcaccttca gcctgccggt aaaatttatc tggctgaaac
     8701 gccgtggttt aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaacg gtgaatcatg
     8761 tgatgattta ttgccggaac cggtactgac ttacattaac caacaaggct tgtatcgctg
     8821 atacctgtcg ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg cgaaacacaa
     8881 gcgaatgtcg atggtctgta cagcggtcat gcgcccacac ccctgaccgc acgcggtatt
     8941 gagcaagcgc aaaatctgca tacactgcta gatgatgttt cctttgacct ggttttatgc
     9001 agtgaactgg aacgggcaca gcataccgcg cgactggttc tcagtgaccg ccagctcccc
     9061 gttcatatca tccctgaact caacgaaatg ttttttggcg actgggagat gcgacatcat
     9121 cgcgacctca tgcaagaaga tgccgaaaac tatagcgcgt ggtgcaatga ctggcagcat
     9181 gctatcccca cgaacggtga aggatttcag gcattttcgc aacgtgtgga acgctttatc
     9241 gcaaggctta gtgaatatca gcactatcag aatattttaa tcgtcagcca tcagggcgta
     9301 ctgagtctgt taattgctcg tttaattggc atgcctgccg agtctatgtg gcattttcgc
     9361 gttgaccagg ggtgttggag cgccattgat atcaaccaaa aattcgcgac gctacgcgtc
     9421 ctcaatagcc gtgccatcgg ggtcgagaat gcatgacttt tctgtttttt tacgggtaag
     9481 ccgcaacgac cattgacagc cccgggcagg ctgatattct ccgcagccag actttttccg
     9541 ccagacacga ctttgtagaa attgttttac aaaaatggcg atgcaatctg cggcgcgggg
     9601 tgggatgata gcccactttc gaaagccgat tcggcgacaa ttgtcccgaa atcgcctctg
     9661 gttcaggtat actgacagac catttttatc tatttgattc acccaggggg aaaacttgca
     9721 gggtaaagca ctccaggatt ttgttatcga caaaattgat gacctcaaag gtcaggacat
     9781 catcgcctta gacgttcagg gcaaatccag catcaccgac tgcatgatca tctgtacggg
     9841 tacgtccagc cgtcatgtta tgtccattgc tgaccacgtt gtgcaggagt ctcgcgcagc
     9901 gggcctgtta ccgctcggcg tagaaggtga aaacagcgcc gactggattg tcgtggattt
     9961 gggcgatgtg attgtccatg tcatgcagga agagagccgt cgcctgtatg aactggaaaa
    10021 actctggagt taatgcgtga agctgcaact tgtcgccgta ggaacgaaaa tgccggactg
    10081 ggtacaaacc ggttttaccg agtacctgcg tcgttttccg aaagatatgc ccttcgagct
    10141 gattgaaatt ccggccggaa aacgcggcaa gaatgcggac atcaagcgca tactcgacaa
    10201 agagggtgag cagatgttgg cggccgcagg caaaaaccgc attgtcaccc tcgatattcc
    10261 aggcaagccc tgggatacgc cgcagttagc cgctgagctg gaacgctgga agctggatgg
    10321 tcgcgacgtc agtctactga ttggcgggcc tgaagggttg tcgcctgcct gtaaagcggc
    10381 ggcggagcag agctggtcgc tgtcggcgct taccctcccc catccgctgg ttcgcgtgct
    10441 ggtcgcagag agtctgtacc gggcgtggag catcaccacc aaccatcctt atcaccgtga
    10501 gtgataaggg agctttgagt agaaaacgca gcggatgaaa ctacagaact cttttcgcga
    10561 ctatacggca gagtccgcgc tgtttgtgcg ccgggcgctg gtcgcctttt tggggatttt
    10621 gctgctgacc ggcgtgctta tcgccaacct gtataatctg caaattgttc gctttaccga
    10681 ctaccagacc cgctctaatg aaaaccgcat taagctggtg cctatcgcgc ccagccgcgg
    10741 cattatctat gaccgtaacg gtatccctct ggccctcaac cgcactatct accagataga
    10801 aatgatgccg gagaaagtcg ataacgtgca gcaaacgctg gacgctttgc gcagcgtggt
    10861 agatctgacc gatgacgata ttgctgcatt ccgaaaagag cgcgcacgtt cacaccgttt
    10921 cacctctatt ccggtgaaaa ctaacctgac cgaagtacaa gtagctcgct ttgccgtcaa
    10981 tcagtaccgt tttccgggtg tcgaagttaa aggctataaa cgtcgttact atccttacgg
    11041 ttcggcgttg acccacgtca tcggctatgt gtcgaaaatc aacgataaag acgtcgaacg
    11101 cctgaataat gacggcaaac tggccaacta tgcggcaacg catgatatcg gtaagctggg
    11161 cattgagcgt tactatgaag acgtgctgca cggtcagacc ggttatgaag aggttgaagt
    11221 taacaaccgt gggcgtgtta tccgccagtt aaaagaagta ccaccgcaag ccggacacga
    11281 tatttacctg acgctggatc tcaaactcca gcaatatatt gaaacgctgc tggcgggtag
    11341 ccgcgcagct gtggtagtca ccgatccgcg tacaggtggg gtgctggcgc tggtttccac
    11401 gcctagttat gacccaaacc tgtttgttga cggtatctcc agcaaagatt attccgcctt
    11461 gctgaatgac ccgaatacac cgctggtgaa ccgcgccaca cagggggttt atcctcccgc
    11521 gtctacagtg aaaccctatg tggcggtttc ggcattgagc gccggggtga tcacgcgcaa
    11581 tacgacgctg tttgacccag gctggtggca actaccaggg tcggaaaaac gttatcgtga
    11641 ctggaaaaaa tggggccacg gacgtctgaa tgtcacaaga tcgctggaag aatctgcgga
    11701 taccttcttc tatcaggtgg cctacgatat ggggatcgat cgcctctccg aatggatggg
    11761 taaattcggt tatggtcatt acaccggtat cgacctggcg gaagaacgtt ccggcaacat
    11821 gcctacccgc gaatggaaac agaaacgctt taaaaaaccg tggtatcagg gtgacaccat
    11881 tccggttggt atcggtcagg gttactggac agcgacccca atccagatga gtaaggcact
    11941 gatgatcctg attaatgacg gtatcgtgaa ggttccgcat ttgctgatga gcaccgccga
    12001 agacggcaaa caggtgccat gggtacaacc gcatgaaccg cccgtcggcg atattcattc
    12061 cggttactgg gagctggcga aagacggtat gtacggtgtt gctaaccgcc ctaacggtac
    12121 ggcgcataaa tactttgcca gcgcaccgta caaaattgcg gcgaaatccg gtaccgctca
    12181 ggtcttcggt ctgaaagcga acgaaaccta taatgcgcac aaaattgccg agcgtttacg
    12241 tgaccacaaa ctgatgaccg cctttgcgcc atacaacaat ccgcaagtgg ctgtcgccat
    12301 gattctggaa aacggcggcg cgggtccggc ggttggtacg ctgatgcgcc agatcctcga
    12361 ccacattatg ctgggtgata acaacaccga tctgcctgcg gaaaatccag cggttgccgc
    12421 agcggaggac cattaatcat gacggataat ccgaataaaa aaacattctg ggataaagtc
    12481 catctcgatc ccacaatgct gctgatttta ctggcattgc tggtttacag cgccctggtt
    12541 atctggagcg ccagcggtca ggatattggc atgatggagc gtaaaatcgg ccaaatcgcg
    12601 atgggtctgg tcatcatggt ggtgatggcg caaattcctc cacgcgttta tgaaggctgg
    12661 gccccctatc tctatatcat ctgtattatt ttgctggtgg cggtagatgc tttcggtgcc
    12721 atctctaaag gcgctcaacg ctggctggac ctcggtattg ttcgttttca gccgtcggaa
    12781 attgccaaaa tagccgtacc actgatggtt gcgcgcttta tcaaccgcga cgtttgcccg
    12841 ccatcgttga agaacactgg catcgcgctg gtgctgatat ttatgcccac gctgctggtg
    12901 gctgcacagc ctgacctggg aacatcaatc ctcgttgcgc tttccggtct gtttgtactg
    12961 ttcctctctg gccttagctg gcgtctgatt ggcgtcgcag tagtgctggt agcggcgttc
    13021 attccgattc tgtggttctt cctgatgcat gattaccagc gccagcgcgt aatgatgctc
    13081 ctggacccgg aatcagaccc actcggcgcg ggctatcaca ttattcagtc taaaattgct
    13141 attggctccg gcggattacg cggcaaaggc tggctgcacg gcactcagtc acagcttgaa
    13201 tttctccccg aacgccatac tgactttatc ttcgcggtac tggcggaaga gctgggatta
    13261 gtgggcattc tgattctgct cgctctctac attctgctga tcatgcgcgg gctgtggata
    13321 gccgccagag cgcaaaccac ctttggtcgc gtcatggctg gtggcttaat gctgatatta
    13381 ttcgtttatg tcttcgtaaa tattggtatg gtaagcggta ttctgccggt tgtaggggtt
    13441 ccgctcccac tggtcagtta tggaggatcg gcgctaattg tgctgatggc tgggttcggg
    13501 attgtaatgt caatccacac ccacaggaaa atgttgtcga aaagcgtgta agaggtgcgc
    13561 aatgcgtaag cagtggctcg ggatctgcat cgcggcagga atgctcgcgg catgtacaag
    13621 cgatgatggt cagcaacaga cggtaagtgt accgcagcct gcggtatgta acggccccat
    13681 agttgaaatt agcggggcgg acccgcgttt cgaaccactg agcgcgacgg caaatcagga
    13741 ttaccagcgc gacggtaaaa gctacaaaat cgtgcaggat ccgtctcgat ttagccaggc
    13801 gggactggcg gcaatctatg atgccgaacc aggcagtaac ctgacggcct ctggcgaagc
    13861 tttcgatccg acacagctga cggcggccca tccaacgctt ccgatcccca gctacgccag
    13921 aatcactaac ctggctaacg ggcgaatgat cgtggtgcgc attaatgatc gcggtcctta
    13981 cggcaacgac cgcgttattt cgctttctcg cgcagcagct gaccgtctta acacgtcaaa
    14041 caacaccaaa gttcgtatcg atccgattat tgtcgcccag gacggttcgc tttctggtcc
    14101 tggtatggct tgtaccacag tcgccaaaca gacttacgcc ctgcctgcac ctcccgattt
    14161 aagcggtggc gcgggaacaa gttcagtgtc tggcccgcag ggtgacattc ttccggtcag
    14221 taattcgacg ctaaaaagcg aagatccgac cggcgcgccg gtaaccagca gcggtttcct
    14281 cggcgcacca acgaccttag cgcctggtgt actggaaggc agcgaaccga cgcctgcgcc
    14341 acagcccgtt gttacagctc cgtcgacaac gcctgcaacc tcgcctgcaa tggtgacacc
    14401 gcaagccgcc tcgcaaagcg ccagcggcaa ctttatggtg caggtcgggg ccgttagcga
    14461 tcaggctcgt gcgcaacagt accaacagca actgagacag aagttcggcg tccccggtcg
    14521 cgtaactcaa aatggcgcag tctggcggat ccagcttggc ccattcgcca gcaaagcaga
    14581 agccagtacc ttgcagcaac gtttgcaaac cgaagcccaa ttacagtcat ttattaccac
    14641 cgcgcagtag cgtaaagcag gcatctgatg tgtcaatttc tgtaaatgac gttaacaaag
    14701 tcacgcggaa agtcagatgc ctgccggtag tgcatttgct atagtagggc acttttttta
    14761 attccatcac ggatgtcgta gttcagacca tgaataccat tttttccgct cgtatcatga
    14821 agcgcctggc gctcaccacg gctctttgca cagcctttat ctctgctgca catgccgatg
    14881 acctgaatat caaaactatg atcccgggtg taccgcagat cgatgcggag tcctacatcc
    14941 tgattgacta taactccggc aaagtgctcg ccgaacagaa cgcagatgtc cgccgcgatc
    15001 ctgccagcct gaccaaaatg atgaccagtt acgttatcgg ccaggcaatg aaagccggta
    15061 aatttaaaga aactgattta gtcactatcg gcaacgacgc atgggccacc ggtaacccgg
    15121 tgtttaaagg ttcttcgctg atgttcctca aaccgggcat gcaggttccg gtttctcagc
    15181 tgatccgcgg tattaacctg caatcgggta acgatgcttg tgtcgccatg gccgattttg
    15241 ccgctggtag ccaggatgct tttgttggct tgatgaacag ctacgttaac gccctgggcc
    15301 tgaaaaacac ccacttccag acggtacatg gtctggatgc tgatggtcag tacagctccg
    15361 cgcgagatat ggcgctgatc ggccaggcat tgatccgtga cgtaccgaat gaatactcga
    15421 tctataaaga aaaagaattt acgtttaacg gtattcgcca gctgaaccgt aacggcctgt
    15481 tatgggataa cagcctgaat gtcgacggca tcaaaaccgg acacactgac aaagcaggtt
    15541 acaaccttgt tgcttctgcg actgaaggcc agatgcgctt gatttctgcg gtgatgggcg
    15601 gacgtacttt taaaggccgt gaagccgaaa gtaaaaaact gctaacctgg ggcttccgtt
    15661 tctttgaaac cgttaaccca ctgaaagtag gtaaagagtt cgcctctgaa ccggtttggt
    15721 ttggtgattc tgatcgcgct tcgttagggg ttgataaaga cgtgtacctg accattccgc
    15781 gtggccgcat gaaagatctg aaagccagct atgtgctgaa cagcagtgaa ttgcatgcgc
    15841 cgctgcaaaa gaatcaggtc gtcggaacta tcaacttcca gcttgatggc aaaacgatcg
    15901 agcaacgccc gctggttgtg ttgcaagaaa tcccggaagg taacttcttc ggcaaaatca
    15961 ttgattacat taaattaatg ttccatcact ggtttggtta aaaattgaac acttgaaagt
    16021 gtaatttccg tccccatata ctaagcatca gtaaaaaaac tcccgccttc tggcgggagt
    16081 tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga
    16141 attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca
    16201 ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag
    16261 cagcaaaggc aactaccact cggtatctat cactatcaac gccactcata tcgagcaggt
    16321 tgaaacactg tatgaagaac tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc
    16381 ttctccgtta cccggcacct ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc
    16441 ccccactttt actcattctc cacggagatg ccgttttgta tcaggataaa attcttgtcc
    16501 gccagctcgg tcttcagcct tacgagccaa tctcccaggc tatgcatgaa ttcaccgata
    16561 cccgcgatga tagtaccctt gatgaaatct ggctggtcga gcactatccg gtattcaccc
    16621 aagggcaggc aggaaaggcg gagcacattt taatgcctgg tgatattccg gtgattcaga
    16681 gcgatcgcgg tgggcaggtg acttatcacg ggccggggca acaggtgatg tatgtattgc
    16741 ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag
    16801 tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg
    16861 tctatgttgg ggaaaagaaa atttgctcac tgggtttacg tattcgacgc ggttgttcat
    16921 tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt
    16981 gtgggtatgc cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta
    17041 ataatattgc accacgttta ctggaaaata ttttagcgct actaaacaat cctgacttcg
    17101 aatatattac cgcttaattt catacctcaa tggcccaatt tacactgggt cattactcat
    17161 aacacctgct aatgctttac atctccttcg cgtttgtata ttttctttat cacttgcaga
    17221 aatgcgatgc tacgccttgc tccgtaactc accccattga ttaacttatt gaaatagttg
    17281 attatgatga gcaaaagcgt tccgttccaa ttagacat
//
LOCUS       DAZKFU020000086        17227 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZKFU020000086 DAZKFU020000000
VERSION     DAZKFU020000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.087"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHOQQ_RS11435"
                     /old_locus_tag="pgaptmp_002287"
     CDS             complement(365..1561)
                     /gene="hemY"
                     /locus_tag="ACHOQQ_RS11435"
                     /old_locus_tag="pgaptmp_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGU4470528.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1564..2769)
                     /gene="hemX"
                     /locus_tag="ACHOQQ_RS11440"
                     /old_locus_tag="pgaptmp_002288"
     CDS             complement(1564..2769)
                     /gene="hemX"
                     /locus_tag="ACHOQQ_RS11440"
                     /old_locus_tag="pgaptmp_002288"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGU4470529.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMNNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPQAAPQAAPQAD
                     TPAAAPQGE"
     gene            complement(2791..3531)
                     /gene="hemD"
                     /locus_tag="ACHOQQ_RS11445"
                     /old_locus_tag="pgaptmp_002289"
     CDS             complement(2791..3531)
                     /gene="hemD"
                     /locus_tag="ACHOQQ_RS11445"
                     /old_locus_tag="pgaptmp_002289"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGU4470530.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3528..4469)
                     /gene="hemC"
                     /locus_tag="ACHOQQ_RS11450"
                     /old_locus_tag="pgaptmp_002290"
     CDS             complement(3528..4469)
                     /gene="hemC"
                     /locus_tag="ACHOQQ_RS11450"
                     /old_locus_tag="pgaptmp_002290"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGU4470531.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4856..7402
                     /gene="cyaA"
                     /locus_tag="ACHOQQ_RS11455"
                     /old_locus_tag="pgaptmp_002291"
     CDS             4856..7402
                     /gene="cyaA"
                     /locus_tag="ACHOQQ_RS11455"
                     /old_locus_tag="pgaptmp_002291"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGU4470532.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPDRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7442..7762)
                     /gene="cyaY"
                     /locus_tag="ACHOQQ_RS11460"
                     /old_locus_tag="pgaptmp_002292"
     CDS             complement(7442..7762)
                     /gene="cyaY"
                     /locus_tag="ACHOQQ_RS11460"
                     /old_locus_tag="pgaptmp_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGU4470533.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VRFR"
     gene            complement(7924..8066)
                     /locus_tag="ACHOQQ_RS11465"
                     /old_locus_tag="pgaptmp_002293"
                     /pseudo
     CDS             complement(7924..8066)
                     /locus_tag="ACHOQQ_RS11465"
                     /old_locus_tag="pgaptmp_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8225..8428
                     /gene="yifL"
                     /locus_tag="ACHOQQ_RS11470"
                     /old_locus_tag="pgaptmp_002294"
     CDS             8225..8428
                     /gene="yifL"
                     /locus_tag="ACHOQQ_RS11470"
                     /old_locus_tag="pgaptmp_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4470534.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8465..9289
                     /gene="dapF"
                     /locus_tag="ACHOQQ_RS11475"
                     /old_locus_tag="pgaptmp_002295"
     CDS             8465..9289
                     /gene="dapF"
                     /locus_tag="ACHOQQ_RS11475"
                     /old_locus_tag="pgaptmp_002295"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGU4470535.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLAYRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9286..9993
                     /gene="yigA"
                     /locus_tag="ACHOQQ_RS11480"
                     /old_locus_tag="pgaptmp_002296"
     CDS             9286..9993
                     /gene="yigA"
                     /locus_tag="ACHOQQ_RS11480"
                     /old_locus_tag="pgaptmp_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HGU4470536.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            9990..10886
                     /gene="xerC"
                     /locus_tag="ACHOQQ_RS11485"
                     /old_locus_tag="pgaptmp_002297"
     CDS             9990..10886
                     /gene="xerC"
                     /locus_tag="ACHOQQ_RS11485"
                     /old_locus_tag="pgaptmp_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGU4470537.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10886..11602
                     /gene="yigB"
                     /locus_tag="ACHOQQ_RS11490"
                     /old_locus_tag="pgaptmp_002298"
     CDS             10886..11602
                     /gene="yigB"
                     /locus_tag="ACHOQQ_RS11490"
                     /old_locus_tag="pgaptmp_002298"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGU4470538.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11686..13848
                     /gene="uvrD"
                     /locus_tag="ACHOQQ_RS11495"
                     /old_locus_tag="pgaptmp_002299"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11686..13848
                     /gene="uvrD"
                     /locus_tag="ACHOQQ_RS11495"
                     /old_locus_tag="pgaptmp_002299"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGU4470539.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13893..14795)
                     /locus_tag="ACHOQQ_RS11500"
                     /old_locus_tag="pgaptmp_002300"
     CDS             complement(13893..14795)
                     /locus_tag="ACHOQQ_RS11500"
                     /old_locus_tag="pgaptmp_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HGU4470540.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTGRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAISFEQVGPELPAKVVELPYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSEKKYEGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(14878..15642)
                     /gene="yigE"
                     /locus_tag="ACHOQQ_RS11505"
                     /old_locus_tag="pgaptmp_002301"
     CDS             complement(14878..15642)
                     /gene="yigE"
                     /locus_tag="ACHOQQ_RS11505"
                     /old_locus_tag="pgaptmp_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGU4470541.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYVKGGAIPWQRYPFVTMISAERKG"
     gene            15804..15860
                     /gene="ysgD"
                     /locus_tag="ACHOQQ_RS11510"
                     /old_locus_tag="pgaptmp_002302"
     CDS             15804..15860
                     /gene="ysgD"
                     /locus_tag="ACHOQQ_RS11510"
                     /old_locus_tag="pgaptmp_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGU4470542.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            16012..16962
                     /gene="corA"
                     /locus_tag="ACHOQQ_RS11515"
                     /old_locus_tag="pgaptmp_002303"
     CDS             16012..16962
                     /gene="corA"
                     /locus_tag="ACHOQQ_RS11515"
                     /old_locus_tag="pgaptmp_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGU4470543.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17005..>17227)
                     /locus_tag="ACHOQQ_RS11520"
                     /old_locus_tag="pgaptmp_002304"
     CDS             complement(17005..>17227)
                     /locus_tag="ACHOQQ_RS11520"
                     /old_locus_tag="pgaptmp_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HGU4470544.1"
                     /translation="MNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNIL
                     GIAVAAYVAVNTNKWFYEKEVKGLNTWSL"
BASE COUNT         3938 a         4515 c         4583 g         4191 t
ORIGIN      
        1 aaatattcac tcaggaagtt attactcagg aagccaagag gattacagaa ttatctcata
       61 acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacga gtagatgctc
      121 attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc agagccgttt
      181 acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc atggacacaa
      241 cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg ctccgtaata
      301 agagcaggcg tttttttatg tgtcaggaag gccccggagg tgcttgcctc cgggtgagaa
      361 ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgcga cgcatcgctg
      421 cagcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg taatcgtagg
      481 cgtccggacg ttgtttcagc gctgcgcgga aggcgagcga tgcttcctgc cattctccgt
      541 gcttcatcag tgactggccc agtgtgctcc acaacagcgg gcgatcgccg acgtttttga
      601 tttgctggcg cagcactttt tccagctgtt ccggattgtt tgttttcagt cgaggaatcg
      661 gcagcagtag gcgatcgtcg tactggcgtt tcaggccatc gatgataatt tgctgggcag
      721 tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac gctacctgat
      781 gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcgcta ccgttatcgg
      841 cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca cgatgttctt
      901 catcaccaac atgggctttc gccattgatg ggataatatc cagcagcgaa ctccatgcac
      961 ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt ggcgtaactt
     1021 ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc agttgcagac
     1081 gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct gcgcgttcca
     1141 gatgttggtt ggcgcgcgct tcatcaccac gttgttgcgc ggcttcggca gccagtagat
     1201 agttcaccac cggttgttca gcgtgatcgg catttttcgc catcagcttt tcaacttgct
     1261 gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgt gcacggcgac
     1321 gcttacgccc gacaaaccac ccacgggtgt gcgcgccagt gcggaagatc cgccgcagta
     1381 gccactcaat ggcaaacagc actaccatcg ccagaatcaa tatgatcgcc aggcccgtga
     1441 cgctggtttc gatattgtag ttgtcggtct ggatcagcac ataaccctga tgaccggcaa
     1501 tcatcgggcc aaccacgatc cccgcaatca gcaacacaaa gagcaataac acttttagca
     1561 tggttattct ccttgcggcg cggctgccgg agtatcagct tgcggtgcag cttgcggtgc
     1621 agcttgcggt gcaggtgcag gtttagcttc cgttgtcccc gctgccggtt gtgccagcag
     1681 gttacgcacg cgagtctgca tcagtttttc cagcatcgcc tggctttgca gggtttccgg
     1741 aagatccatc gagatatttt gctggcttaa ctggtccacc tcgtcgagga acgctttggt
     1801 ggtggcatca tcagtatcgt agtaagcacg tacccaggtg gagacgttct ccagcgcctg
     1861 gcgataagtc tcttgctggt gacgcggtac agcttgtgct gcgaccagca ggcgagagcg
     1921 aatattttcg cgcagataga tatcctgatt tggcgctaac agcggtacgg cggtgtcatc
     1981 acgacggcga atcgtaatga agttgttcat aaagttctgc cagctttttt gcagattgat
     2041 acgccattcg ctgatggaac tggaaagctc ttcaccgtcg gaatccatcg gcgaaccatc
     2101 gctgtcatta tcggccagac gcaggttatc tacctgattt gaaagctgat taagcttaag
     2161 gatgatgccg tcataatcca cctgcgatac tgcagaaagg ctggcgatat catcggtaat
     2221 tgcccgacga acggtaatca gactcgggtc attcatatcc gccaggctgg cgtctgcact
     2281 tttcagcaac gctgcagcgg tcgtgacgtc ctgatcgctc cacagcttcc gtccggcgag
     2341 tttcaccaga aaatcggcct gagccagcag ccaggtttta gcatcgctgc cggaaatggt
     2401 ggcgaccttt tgttggactt catccaactg ttttgccagc gtttcttgct gacgattcgc
     2461 ctgcttaagt tgtgcagctt gttgcttaat aatgccttcc agctcggctt tttggctctc
     2521 ctgggctttt tgcaatgccg tcagttggtt agccagggca tcgctggtgg cggtctgatt
     2581 gacggcctgt tgtttacccc agccatacaa accgacgcct gccgccagag caatagcgat
     2641 agccaccgca ctgagaatca atgcggtatt gttcttactc tttttttctg ttgcgacagg
     2701 ttgtgacgtg gtgtccacgg cctccctggt ctcttcaacc acggcggagg ttttttcttg
     2761 ttccgtcatt atggcttcct gttatgagag ttattgtaat gcccgtaaaa gcgcatcgtt
     2821 gtcagcgtta tcggcgacct taatgtcttg ccagcccagt tcccgggcga gtttcgccaa
     2881 acgctcactg acgaccaata gtcgacagtg tagtaaccag tgctcacgat accattgtgg
     2941 gatcagcgac cagagttgct gcaacatttc accgctggta acaacgaccg tcgtcacctc
     3001 gcgggattgc cagcgcatcg cttcttctgc accatcgtaa tggattgcgc atcgttgata
     3061 acattcacaa aaagtgacct cagcaccgcg cgccgtcagg gtatccccaa ttagctcacg
     3121 accgccattg ccacgtaata tcagcgcacg tttgcccgca atattttgta attcaggtaa
     3181 ttgtagcaag acttcgctga tttcccgatc ctgtgggtag agaatcttct gtccgcttac
     3241 ggtatgtagc gccagtgcgg tggtgcgtcc aatggcgaaa taagtaggta gtcggggcca
     3301 tttacgatct tgctgatgca gctgtgattg ggcaaaagca accgcgtgtt gcgagagggc
     3361 aaacaacaga tcgctctccc ccagcgctgc cagttgatca gcaagttgcg gtaattgtcg
     3421 acccggagaa aactcaatca gcggaaaatg ccaggccacc tgccccagtg tgcgcagacg
     3481 gctcactaac tcttctccag cgggagacgg gcgggtgaca aggatactca tgccggggcg
     3541 tctccgttat agacttcagc gaggatctcg cgcgcgccgt tattcagtag ctcttctgcc
     3601 agcgaaatcc ccatttgttc ggcattttgc ggcgcaccgc ggcgttcacc gcgaataatc
     3661 tgcgaaccgt ccggcgcgcc gaccagcgca cgcagccaga tttcgccatc aataagctcg
     3721 gcgtagctac caattggcac ctgacatccg ccttcgagac gggtattcat ggcgcgttct
     3781 gcggtaacgc gcagtgcagt ttcgtggtga ttcagcgcgg caagcagctc gcgagtgcgt
     3841 gtatcatcaa ggcggcattc aataccgact gcaccttgtc ctaccgccgg aagagaaatc
     3901 tcgggtggca acgcggcgcg aatgcgggac tccagaccta aacgttttag tccggctacg
     3961 gcaagaatga tggcatcgta ttcgccgtta tccagtttgc tcaggcgagt gccgacgttg
     4021 ccgcgcaggg agcggataat cagatccggg cggcgttcag ccagttggca ctggcgacgt
     4081 aaactggacg tcccgacgat actgcctgcc ggtaacgcat ccagactgtc atagttattg
     4141 gacacaaagg catcgcgagg atcttcacgc tcgcaaatag tgaccagccc cagaccttgc
     4201 gggaattcaa ccggcacatc tttcattgaa tgtacggcga tatcggcgcg attttcgagg
     4261 agcgcgactt ccagctcttt tacaaataag ccttttccgc cgactttcgc cagcggcgta
     4321 tcaagaatca catcgccgcg cgtcaccatc ggtaccagtt caacgaccag gcccggatgg
     4381 ctcgccatca acttgtcttt gacatagtgt gcctgccaga gtgcaagtgg gctttggcgt
     4441 gtggcaattc ttaaaacatt gtctaacatg cttgttaccg tcattatcat ccgtggtcca
     4501 tcctaacatc cttgccagag tgatgtcagt gttgtggtga aacgtagacg cctgcgcaaa
     4561 ccgtaaaatg aggtctggca gtggatcctg acaggcgttt cacgccgtcg taataaggaa
     4621 tttacagaga ataaacggtg ctacacttgt atgtagcgca tctttcttta cggtcaatca
     4681 gcaaggtgtt aaattgatca cgttttagac cattttttcg tcgtgaaact aaaaaaacca
     4741 ggcgcgaaaa gtggtaacgg ttacctttga catacgaaat atcccgaatg ccgcgtgtta
     4801 ccgttgatgt tggcggaatc acagtcatga cgggtagcaa atcaggcgat acgtcttgta
     4861 cctctatatt gagactctga aacagagact ggatgccata aatcaattgc gtgtggatcg
     4921 cgcgcttgct gctatggggc ctgcattcca acaggtctac agtctactgc cgacattgtt
     4981 gcactatcac catccgctaa tgccgggtta ccttgatggt aacgttccca aaggcatttg
     5041 cctttacacg cctgatgaaa ctcaacgcca ctacctgaac gagcttgaac tgtatcgtgg
     5101 aatgtcagta caggacccgc cgaaaggtga gcttccaatt actggtgtat acaccatggg
     5161 cagcacctcg tccgtagggc aaagttgttc ctctgacctg gatatctggg tctgtcatca
     5221 atcctggctc gatagcgaag agcgccagtt gctacaacgt aaatgcagcc tgctggaaag
     5281 ctgggccgcc tcgctgggtg tggaagttag cttcttcctg attgatgaaa accgttttcg
     5341 ccacaacgag agtggcagtc tggggggcga agattgtggc tccacccagc atatactgct
     5401 gcttgatgaa ttttatcgta ccgccgtgcg tctcgccggt aagcgtattc tgtggaatat
     5461 ggtgccgtgc gacgaagaag agcattacga tgactacgta atgacgctct acgcacaagg
     5521 cgtgctgacg ccaaatgaat ggctggatct cggtggctta agctcgcttt ctgctgaaga
     5581 gtactttggt gccagccttt ggcagctcta caagagtatc gattccccat acaaagcggt
     5641 actgaaaaca ctgctgctgg aagcctattc ctgggaatac ccgaacccac gtctgctggc
     5701 gaaagatatc aaacagcgtt tgcacgacgg cgagattgta tcgtttggtc tcgatccata
     5761 ctgcatgatg ctggagcgtg ttactgaata cctgacggcg attgaagatt ttacccgtct
     5821 ggatttagta cgtcgctgct tctatttaaa agtgtgcgaa aagctcagcc gtgaacgcgc
     5881 ctgcgtaggc tggcgtcgcg cagtgttgag ccagttagtg agcgagtggg gttgggacga
     5941 agctcgtctg gcaatgctcg ataaccgcgc taactggaag attgatcagg tgcgtgaggc
     6001 gcacaacgag ttgctcgacg cgatgatgca gagctaccgt aatctgatcc gctttgcgcg
     6061 tcgcaataac cttagcgtct ccgccagtcc gcaggatatc ggcgtgctga cgcgtaagct
     6121 gtatgccgcg tttgaagcat taccaggtaa agtgacgctg gtaaacccgc agatttcacc
     6181 cgatctctcg gaaccgaatc tgacctttat ttatgtgccg ccggaccggg ctaaccgttc
     6241 aggttggtat ctgtataacc gcgcgccaaa tattgagtcg atcatcagcc atcagccgct
     6301 ggaatataac cgttacctga ataagctggt ggcgtgggca tggtttaatg gcctgctgac
     6361 ctcgcgcacc cgtttgtata ttaaaggtaa cggcattgtc gatttgccta agttgcagga
     6421 gatggtcgcc gacgtgtcgc accatttccc gctgcgctta cctgcaccga caccgaaggc
     6481 gctctatagc ccgtgtgaga tccgccatct ggcgattatc gttaacctgg aatatgaccc
     6541 gacagcggcg ttccgcaatc aggtggtgca tttcgatttc cgtaagctgg atgtcttcag
     6601 ctttggcgag aatcaaaatt gcctggtagg tagcgttgac ctgctgtacc gcaactcgtg
     6661 gaacgaagtg cgtacgctgc acttcaacgg cgagcaatcg atgatcgaag ccctgaaaac
     6721 tattctcggc aaaatgcatc aggacgccgc accgccagat agcgtggaag tcttctgtta
     6781 tagccagcat ctgcgcggct taattcgtac tcgcgtgcag caactggttt ctgagtgtat
     6841 tgaattgcgt ctttccagca cccgccagga aaccgggcgt tttaaggcgc tgcgcgtttc
     6901 tggtcaaacc tggggcttgt tcttcgaacg cctgaatgta tcggtacaga aactggagaa
     6961 cgccatcgag ttttatggcg ctatttcgca taacaaactg cacggcctgt cagtgcaggt
     7021 tgaaaccaat cacgtcaaat taccggcggt ggtggacggc tttgccagcg aagggatcat
     7081 ccagttcttt ttcgaagaaa cgcaagatga gaatggcttt aatatctaca ttctcgacga
     7141 aagcaaccgg gttgaggtgt atcaccactg cgaaggcagc aaagaggagc tggtacgtga
     7201 cgtcagtcgc ttctactcgt catcgcatga ccgttttacc tacggctcaa gcttcatcaa
     7261 cttcaacctg ccgcagttct atcagattgt gaaggttgat ggtcgtgaac aggtgattcc
     7321 gttccgcaca aaatctatcg gtaacatgcc gcctgccaat caggatcacg atacgccgct
     7381 attacagcag tatttttcgt gatgaacgtg ccggaaagcg aggcttatcc ggcatgcaat
     7441 cttagcggaa tctgactgtt tcacccgcct gctgcgtcgc cgcctgttcc agcaaatccc
     7501 agaaggtttc gccgctgcga tcgcaaatcc actcatcgcc tttcagatca aaatggtagc
     7561 cgccttgttt ggttgccagc catacctggt gcagcggttc ctggcggttg ataatgattt
     7621 tgctgccgtt ttcaaaggta atggtcagta caccgccgtt gatttcgcag tcgatatcgc
     7681 tgtcgccatc ccagtcgtcc agacgctctt caatggtcag ccacagttga tcagccaggc
     7741 gatgaaattc actgtcgttc attgttgtat cctgttttta agtgatggcg gcagtatagc
     7801 ggcatggggt cagggcttca aagtttgcac ctctgcggct gcgttacggc atgattcatc
     7861 cgtcaccgga ataatgatgt ctctgtgtag cgaaagaatt gtctcttcat tagggcgcag
     7921 ttacaccacg cctttccctg tttctggtaa acattatgat caggtttacc gagcgagcat
     7981 cctcacgctg acggaactaa aaaagccaac aaacaaacca cattgcgata gtgcataaag
     8041 ccatcctggc gcgaggtgcc gatcacgaaa ctaccagcaa aacataaatc cccacgagta
     8101 agcgttatac tcgcagcatt tcctcacttt tcagacttca taaagagtcg ctaaacgctt
     8161 gcttttacgc cttctcctgc gatgatagaa agcagaaagc gatgaacttt acaggcaatc
     8221 tataatgaaa aacgtgttta aggcactcac tgtattactt actctcttca gcctgacggg
     8281 ctgcggtctg aaaggtccgc tctatttccc gcctgcagat aaaaacgcac cgccgccgac
     8341 caaaccggta gagacgcaaa cgcaatccac ggtgccggat aaaaacgatc gcgccactgg
     8401 cgatggtcca tcccaggtga attactaaaa gtcagtttct gtacccgcgt gattggagta
     8461 aatgatgcag ttctcgaaaa tgcatggcct tggcaacgat tttatggtcg tcgacgcggt
     8521 aacgcagaat gtcttttttt caccggagct gattcgtcgc ctggcgtatc ggcacctggg
     8581 ggtagggttt gaccagctgc tggtggttga gccgccgtat gatcctgaac tggattttca
     8641 ctatcgcatt ttcaatgctg atggcagtga agtggcgcag tgcggcaacg gtgcgcgctg
     8701 ctttgcccgt tttgtgcgcc tgaaagggct gaccaataag cgcgatatcc gcgtcagcac
     8761 cgccaacggg cggatggttc tgaccgtcac cgatgatgat ctggtccgcg taaatatggg
     8821 cgaacccaac ttcgaacctt ccgctgtgcc gtttcgcgct aacaaagcgg aaaagaccta
     8881 cattatgcgc gccgccgagc agacaatctt atgcggcgtg gtgtcgatgg gaaatccgca
     8941 ttgcgtgatt caggtcgatg atgtcgatac cgcggcggta gaaacgcttg gtcctgttct
     9001 ggaaagccac gagcgttttc cggagcgagc caatatcggt tttatgcaag tggttaagcg
     9061 cgagcatatt cgtttacgcg tttatgagcg tggggcagga gaaacccagg cctgcggcag
     9121 cggcgcatgt gcggcggtgg cggtagggat tcagcaaggt ttgctggccg aagaagtacg
     9181 cgtggaactc cccggcggtc gtcttgatat cgcctggaaa ggtccgggtc acccgttata
     9241 tatgactggc ccggcggtac atgtctacga cggatttatt catctatgaa gcaaccaggg
     9301 gaagaactgc aggaaacact cacggagctt gatgaccggg cggttgtcga ttatctgatt
     9361 aaaaatcctg agttttttat ccgcaatgcg cgcgcagtag aagcgatacg tgtgccgcat
     9421 ccggtacgcg gcaccgtttc gttggtcgag tggcacatgg cccgcgcacg taatcatatt
     9481 catgttctgg aagagaacat ggcgctgttg atggaacagg ctatcgccaa cgaaggcctg
     9541 ttttatcgct tactctacct gcagcgcagt ctcaccgccg ccagcagtct cgacgatatg
     9601 ctgatgcgct ttcaccgctg ggcgcgcgat ctcggcctgg caggtgcgag tctgcgcctg
     9661 tttccggatc gctggcgctt aggtgcgccg tcgaaccaca ctcatctggc attaagccgt
     9721 cagtctttcg aaccgctgcg tattcagcgt ttggggcagg aacagcacta tcttgggccg
     9781 cttaacggac cagagctgct ggtggtgcta ccggaagcga aagcggtggg atcggtggcg
     9841 atgtcgatgc tgggaagcga tgctgatttg ggtgtcgtgc tgtttaccag tcgcgatgcc
     9901 agtcactatc aacaagggca gggaacgcag ttacttcatg aaattgcgct gatgttgccg
     9961 gagcttctgg agcgttggat tgaacgcgta tgaccgattt acacaccgat gtagaacgct
    10021 acctacgtta tctgagcgtg gagcgtcagc ttagcccgat aaccctactt aactaccagc
    10081 gtcagcttga ggcgatcatc aattttgcca gcgaaaacgg cctgcaaagc tggcaacaat
    10141 gcgatgcagc gatggtgcgc aattttgctg tacgcagtcg ccgtaaaggg ctgggagcag
    10201 caagcctcgc gttacggctt tctgcgctac gtagcttttt tgactggctg gtcagccaga
    10261 acgaactcaa agctaacccg gcgaaaggtg tttcggcacc gaaagcgccg cgtcatctgc
    10321 cgaaaaacat cgacgtcgac gatatgaatc ggctgctgga tattgatatc aacgatcccc
    10381 tcgctgtacg cgaccgtgca atgctggaag tgatgtacgg cgctggtctg cgtctttccg
    10441 agttggtggg gctggatatc aaacacctcg acctggagtc tggtgaagtg tgggtaatgg
    10501 ggaaaggcag caaagagcgc cgcctgccga ttggtcgcaa cgctgtggcg tggattgagc
    10561 actggcttga tttgcgcgac ctgtttggta gcgaagacga cgcgcttttt ctgtcgaaac
    10621 tgggcaagcg tatctccgcg cgtaatgtgc agaaacgctt tgccgaatgg ggcataaaac
    10681 aagggctgaa taatcacgtt cacccgcata aattacgtca ctcgttcgct acgcatatgc
    10741 tggagtcgag cggcgatctt cgtggtgtgc aggagctgct gggtcatgcc aacctctcca
    10801 ccacgcaaat ctatactcat cttgattttc aacaccttgc ctcggtgtac gatgcggcgc
    10861 atccacgcgc caaacggggg aaataatgcg tttttaccgg cctttggggc gcatctcggc
    10921 gctcaccttt gacctggatg atacccttta cgataaccgt ccggtgattt tgcgcaccga
    10981 gcgagaggcg cttacctttg tgcaaaatta tcatccggcg ctgcgcagct tccagaatga
    11041 agatctgcaa cgcctgcgcc aggcggtacg ggaagcggaa cccgagattt atcacgacgt
    11101 gacgcgttgg cgttttcgtt cgattgaaca agcgatgctc gacgccgggc tgagtgccga
    11161 agaagccagt gcaggcgcac acgcagcaat gattaacttt gccaaatggc gcagccgaat
    11221 cgacgtcccg cagcaaactc acgacacctt aaaacagctg gcgaaaaaat ggccgctggt
    11281 ggcgatcacc aacggtaacg cccagccgga actgtttggt ttgggggatt attttgagtt
    11341 tgtgctgcgc gctggcccgc acgggcgctc aaaaccgttc agcgatatgt actttttggc
    11401 tgcggaaaaa ctcaacgtgc cgatcggcga gatcttacat gttggggacg atctcaccac
    11461 tgacgtgggt ggggcaattc gcagcggaat gcaggcttgt tggatcagac cggaaaatgg
    11521 cgatctgatg caaacctggg atagccgttt actgccgcat ctggaaattt cccggttggc
    11581 atctctgacc tcgctgatat aatcagcaaa tctgtatata tacccagctt tttggcggag
    11641 ggcgttgcgc ttctccgccc aacctatttt tacgcggcgg tgccaatgga cgtttcttac
    11701 ctgctcgaca gccttaatga caaacagcgc gaagcggtgg ccgcgccacg cagcaacctt
    11761 ctggtgctgg cgggcgcggg cagtggtaag acgcgcgtac tggtgcatcg tatcgcctgg
    11821 ctgatgagcg tggaaaactg ctcgccatac tcgattatgg ctgtgacgtt taccaacaaa
    11881 gcggcggcgg agatgcgtca tcgtatcggg caactgatgg gcaccagcca gggcggcatg
    11941 tgggtcggca ccttccacgg gctggcgcac cgcctgctgc gtgcgcacca tatggacgcc
    12001 aatctgccgc aggatttcca gatcctcgac agtgaagacc agctacgcct gcttaagcgt
    12061 ctgatcaaag ccatgaacct cgacgagaag cagtggccgc cgcgccaggc aatgtggtac
    12121 atcaacagcc agaaagatga aggcttacgt ccgcatcata ttcaaagcta cggtaatccg
    12181 gtggagcaga cttggcagaa agtgtatcag gcgtatcagg aagcgtgcga ccgtgcgggc
    12241 ctggtggact tcgccgagct gctgctgcgt gctcacgagt tgtggcttaa caagccgcat
    12301 atcctgcaac actaccgcga acgttttact aacatcctgg tggacgaatt ccaggatacc
    12361 aacaacattc agtacgcgtg gatccgcctg ctggcgggcg acaccggcaa agtgatgatc
    12421 gtcggtgatg acgaccagtc aatctacggc tggcgcgggg cgcaggtgga gaatattcag
    12481 cgtttcctta atgatttccc cggtgccgaa actattcgtc tggagcaaaa ctaccgctct
    12541 accagcaata ttctgagcgc cgctaacgcc ctgattgaaa acaataacgg gcgtctgggt
    12601 aaaaaactgt ggaccgatgg cgcggacggt gagcctattt ccctctattg cgcttttaac
    12661 gaactcgatg aagcgcgttt tgtggttaac cgcatcaaaa cctggcagga caacggcgga
    12721 gcgcttgccg agtgcgccat tctctaccgc agcaacgccc agtcgcgtgt actggaagag
    12781 gccttattac aggcgagtat gccgtaccgt atttacggcg ggatgcgctt cttcgaacgc
    12841 caggaaatca aagatgcgct ctcgtatctg cgcctgattg ccaaccgcaa cgacgacgcg
    12901 gcctttgagc gcgtagtgaa tacaccaacg cggggtattg gtgaccggac gctggacgtg
    12961 gtacgtcaga catcgcgcga tcgccagtta acactctggc aggcatgtcg tgaactgttg
    13021 caggaaaaag ccctcgccgg acgtgcagcc agtgccttgc agcggtttat ggaactgatc
    13081 gacgccttag cgcaggaaac tgccgatatg ccgctgcatg tacagactga ccgggtaatt
    13141 aaagactccg gcctgcgtac catgtacgag caggagaagg gcgaaaaagg tcagacgcgt
    13201 atcgaaaact tagaggaact ggtgacggca acgcgccagt tcagctacaa cgaagaagac
    13261 gaagatttaa tgccgctgca ggcgttcctc tcccatgcgg cgctggaagc gggcgagggg
    13321 caggcggata cctggcagga cgcggtgcag ttgatgacgc tacactcggc gaaaggcctg
    13381 gagttcccgc aggtgtttat cgtcggtatg gaagagggca tgttcccaag ccagatgtcg
    13441 ctggatgaag gcggtcgtct ggaagaagaa cgccgtctgg cctacgttgg cgtaacccgt
    13501 gcgatgcaga aactgacgct gacctacgcg gaaactcgcc gtctgtatgg caaagaggtt
    13561 taccatcgcc cgtcgcgctt tatcggtgag ttgccggaag agtgtgtgga agaggtgcgc
    13621 ctgcgcgcca cggtaagccg cccggtcagc catcagcgta tgggtacgcc gatggtcgag
    13681 aacgacagcg gctacaaact tggccagcgc gtgcgccacg ccaagtttgg tgaaggcact
    13741 atcgtcaata tggaaggcag cggcgagcat agccgtttgc aggtggcatt ccagggccag
    13801 ggtattaaat ggctggtggc ggcttacgcc cggctggaga cggtataacg ttgccggatg
    13861 cggtgcgcca caccgcatcc ggcatcagca ccttacttat cacatttctt cacatcaata
    13921 ttccagtcag caccagacgc aacctgatgt ttctgcgcga aattatccat attcagcgcc
    13981 acggaaacat tcaataagct gttcagatac accatcggct gtccttccgg cacatcgccg
    14041 aagctggcga catacggcat tttcccttcg tattttttct cggaaccttc ggaaatggtc
    14101 acacataacg tatcgttgag tttgattcct gcctgattca gcaactcgtc gctgatattg
    14161 ctccagacgt taccgtactg aatatcgaga atcgggatat tgcccttcac ttcacctttt
    14221 gtcgctttcg ctttctgata aggcagttcg acaacttttg ccggaagctc tggccccacc
    14281 tgctcaaagg aaatcgcgcc ggaagccaga cgcgcccctg tgtaggcata tacatcgcga
    14341 ccatggaagg tatatgactt ttcggaacct ttcaggcggt tggttttttc atcgatttgc
    14401 cggacgctct caatacccag cgactccgcc accaaagtga gcgtaccgtt atccggtgaa
    14461 acgaagtatt gaccgttttt ggttttcagc actaccgatt tacggcctgt acctacacct
    14521 ggatcgacca cgctaacaaa caccgaacct tgtggccaat aactggcggt ctgatacagg
    14581 cggtaagcgc cttcccagat gttatacggt ggaatttcgt gtgttaaatc aaagatttta
    14641 agattatggt caacaccgaa tgcgacccct ttcatcgccg agacggcacc atctttcagg
    14701 ctaaaatcag tttgtaaaat caaagcgtta tccgcataag cggatgatga tcccagacac
    14761 gttaataaaa ataacgttgc gagcgttttt ttcatttttg tttcccttca tttatctgat
    14821 gattttccag ctgtgtagcc agccccagtt tgtctggcct cctcgagtgt acaatgctta
    14881 acctttccgc tcggcggaaa tcatggtgac aaaaggatag cgttgccacg ggattgcccc
    14941 gcctttcaca tacatatgtg aaatcgtgcc atcaagataa agcagctgct caacgttcag
    15001 tttcgctttg gcataacagg caaaatcata aaaatttgtc gcctgctggc tcaacaaaaa
    15061 cacggcattc ccatgtttat taatcccaac accgttacga attttacgtg aggcgacgtt
    15121 gggatgaata cgcggattaa ttacactgtt ttccatcaac attggccctg actgcaccgc
    15181 aaactgaatc tctttactgg ttttgaaggc atccagacga atgatgccga ctttatctcc
    15241 cgcgacataa aacacgccgc caggacggat aaagaaattc ccttcacctg aagcgagatt
    15301 taacgccacc ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc
    15361 atagatgccg ccgttcatcg ccatctgcac ctgaccctga ctattcatat ccgccagcag
    15421 agcatgtaaa gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc
    15481 tgtttgagga ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc
    15541 atcagcggca acagcaaaca agggaagtaa ggtgagggcg agaaaaatcc gtttgagatt
    15601 caaggtgatc attcctttac caatgagtag ccgatgcgcc attataggtc ctggatgtgg
    15661 gattttttta tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg
    15721 ttctgtggtg ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg
    15781 atcactctaa gaggacattc gccttggaca cacccagtag atactggctc actatcctgt
    15841 catccaggat caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg
    15901 acctcaattt ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt
    15961 tctgtgtgcc caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc
    16021 gcatttcaac tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt
    16081 gtaaatgcag tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa
    16141 tctgaacttg gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca
    16201 cgtttctttg aagacgatga cggcctgcat attcactcct tcttcttctt tgaagatgcg
    16261 gaagatcacg ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact
    16321 ctgcgtgagc gtgaactgcc tgcattccgt ctgtatcgta tgcgcgcccg tagccagtca
    16381 atggtggacg gtaacgccta cgagctgctg ctggatctgt tcgaaaccaa aatcgaacag
    16441 ttggcagatg aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg
    16501 gaagggcatc agggcgatga gtacgacgag gcgctctcca ctctggcgga actggaagat
    16561 atcggctgga aagtgcgcct gtgtctgatg gatacccagc gtgcgctcaa cttcctggtg
    16621 cgtaaggcgc gtttaccggg tggtcaactg gagcaggcgc gtgaaatcct gcgagatatc
    16681 gaatccctgc tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg
    16741 gcaatgggtt ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc
    16801 gtcgtgttcc tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg
    16861 ccagaactga agtggagttt cggctaccca ggcgcgatta tctttatgat cctcgcgggc
    16921 ctggcaccgt atctgtactt taagcggaag aactggttgt aaaaaacgag agcggtggtt
    16981 tagcctggct aagccacctg ttattcaaag gctccaggta tttaaccctt ttacctcttt
    17041 ctcataaaac catttgttcg tgttaacagc aacataggct gctacggcaa ttcccagaat
    17101 gttaacgcca attaatgcac caacaaatag agatagaatg ccaagtaata gaactataat
    17161 tgcttttttc cataagccca gaacaaataa atagatccag gaacagaaga aagcaatgaa
    17221 attcatt
//
LOCUS       DAZKFU020000087        17113 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZKFU020000087 DAZKFU020000000
VERSION     DAZKFU020000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.088"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHOQQ_RS11525"
                     /old_locus_tag="pgaptmp_002305"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHOQQ_RS11525"
                     /old_locus_tag="pgaptmp_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HGU4470545.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHOQQ_RS11530"
                     /old_locus_tag="pgaptmp_002306"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHOQQ_RS11530"
                     /old_locus_tag="pgaptmp_002306"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGU4470546.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(938..1405)
                     /locus_tag="ACHOQQ_RS11535"
                     /old_locus_tag="pgaptmp_002307"
     CDS             complement(938..1405)
                     /locus_tag="ACHOQQ_RS11535"
                     /old_locus_tag="pgaptmp_002307"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU4470547.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHOQQ_RS11540"
                     /old_locus_tag="pgaptmp_002308"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHOQQ_RS11540"
                     /old_locus_tag="pgaptmp_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGU4470548.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHOQQ_RS11545"
                     /old_locus_tag="pgaptmp_002309"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHOQQ_RS11545"
                     /old_locus_tag="pgaptmp_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGU4470549.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHOQQ_RS11550"
                     /old_locus_tag="pgaptmp_002310"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHOQQ_RS11550"
                     /old_locus_tag="pgaptmp_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGU4470550.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHOQQ_RS11555"
                     /old_locus_tag="pgaptmp_002311"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHOQQ_RS11555"
                     /old_locus_tag="pgaptmp_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGU4470551.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHOQQ_RS11560"
                     /old_locus_tag="pgaptmp_002312"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHOQQ_RS11560"
                     /old_locus_tag="pgaptmp_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGU4470552.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHOQQ_RS11565"
                     /old_locus_tag="pgaptmp_002313"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHOQQ_RS11565"
                     /old_locus_tag="pgaptmp_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGU4470553.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHOQQ_RS11570"
                     /old_locus_tag="pgaptmp_002314"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHOQQ_RS11570"
                     /old_locus_tag="pgaptmp_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGU4470554.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHOQQ_RS11575"
                     /old_locus_tag="pgaptmp_002315"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHOQQ_RS11575"
                     /old_locus_tag="pgaptmp_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGU4470555.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHOQQ_RS11580"
                     /old_locus_tag="pgaptmp_002316"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHOQQ_RS11580"
                     /old_locus_tag="pgaptmp_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGU4470556.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHOQQ_RS11585"
                     /old_locus_tag="pgaptmp_002317"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHOQQ_RS11585"
                     /old_locus_tag="pgaptmp_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGU4470557.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHOQQ_RS11590"
                     /old_locus_tag="pgaptmp_002318"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHOQQ_RS11590"
                     /old_locus_tag="pgaptmp_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGU4470558.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHOQQ_RS11595"
                     /old_locus_tag="pgaptmp_002319"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHOQQ_RS11595"
                     /old_locus_tag="pgaptmp_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGU4470559.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHOQQ_RS11600"
                     /old_locus_tag="pgaptmp_002320"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHOQQ_RS11600"
                     /old_locus_tag="pgaptmp_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGU4470560.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHOQQ_RS11605"
                     /old_locus_tag="pgaptmp_002321"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHOQQ_RS11605"
                     /old_locus_tag="pgaptmp_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGU4470561.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHOQQ_RS11610"
                     /old_locus_tag="pgaptmp_002322"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHOQQ_RS11610"
                     /old_locus_tag="pgaptmp_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGU4470562.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHOQQ_RS11615"
                     /old_locus_tag="pgaptmp_002323"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHOQQ_RS11615"
                     /old_locus_tag="pgaptmp_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGU4470563.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHOQQ_RS11620"
                     /old_locus_tag="pgaptmp_002324"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHOQQ_RS11620"
                     /old_locus_tag="pgaptmp_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGU4470564.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHOQQ_RS11625"
                     /old_locus_tag="pgaptmp_002325"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHOQQ_RS11625"
                     /old_locus_tag="pgaptmp_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGU4470565.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHOQQ_RS11630"
                     /old_locus_tag="pgaptmp_002326"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHOQQ_RS11630"
                     /old_locus_tag="pgaptmp_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGU4470566.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHOQQ_RS11635"
                     /old_locus_tag="pgaptmp_002327"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHOQQ_RS11635"
                     /old_locus_tag="pgaptmp_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGU4470567.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHOQQ_RS11640"
                     /old_locus_tag="pgaptmp_002328"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHOQQ_RS11640"
                     /old_locus_tag="pgaptmp_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGU4470568.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHOQQ_RS11645"
                     /old_locus_tag="pgaptmp_002329"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHOQQ_RS11645"
                     /old_locus_tag="pgaptmp_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGU4470569.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHOQQ_RS11650"
                     /old_locus_tag="pgaptmp_002330"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHOQQ_RS11650"
                     /old_locus_tag="pgaptmp_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGU4470570.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHOQQ_RS11655"
                     /old_locus_tag="pgaptmp_002331"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHOQQ_RS11655"
                     /old_locus_tag="pgaptmp_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGU4470571.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHOQQ_RS11660"
                     /old_locus_tag="pgaptmp_002332"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHOQQ_RS11660"
                     /old_locus_tag="pgaptmp_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGU4470572.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHOQQ_RS11665"
                     /old_locus_tag="pgaptmp_002333"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHOQQ_RS11665"
                     /old_locus_tag="pgaptmp_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGU4470573.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHOQQ_RS11670"
                     /old_locus_tag="pgaptmp_002334"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHOQQ_RS11670"
                     /old_locus_tag="pgaptmp_002334"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGU4470574.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHOQQ_RS11675"
                     /old_locus_tag="pgaptmp_002335"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHOQQ_RS11675"
                     /old_locus_tag="pgaptmp_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGU4470575.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHOQQ_RS11680"
                     /old_locus_tag="pgaptmp_002336"
     CDS             15545..15913
                     /locus_tag="ACHOQQ_RS11680"
                     /old_locus_tag="pgaptmp_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGU4470576.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHOQQ_RS11685"
                     /old_locus_tag="pgaptmp_002337"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHOQQ_RS11685"
                     /old_locus_tag="pgaptmp_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGU4470577.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHOQQ_RS11690"
                     /old_locus_tag="pgaptmp_002338"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHOQQ_RS11690"
                     /old_locus_tag="pgaptmp_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGU4470578.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHOQQ_RS11695"
                     /old_locus_tag="pgaptmp_002339"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHOQQ_RS11695"
                     /old_locus_tag="pgaptmp_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGU4470579.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
BASE COUNT         4452 a         3992 c         4526 g         4143 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaaagatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccacaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaatgattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttcta cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaggac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt gatggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttccgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgct acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgtcgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta caacgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaatgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgtcatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcag cggagggaag caattcagct tctggatatc ctgagaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tggacaaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctagagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact gacaggatta cactcgcgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgagt agagaaaaat
    16501 aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggcc ttctgctttc aggcgtttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccttgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagag gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat cct
//
LOCUS       DAZKFU020000088        16862 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZKFU020000088 DAZKFU020000000
VERSION     DAZKFU020000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.089"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(131..1150)
                     /locus_tag="ACHOQQ_RS11700"
                     /old_locus_tag="pgaptmp_002340"
     CDS             complement(131..1150)
                     /locus_tag="ACHOQQ_RS11700"
                     /old_locus_tag="pgaptmp_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4470580.1"
                     /translation="MGRPRKNKKDNALPPRVRSNGYSYVWKPEGSTKTIGLGRVRETS
                     IAKVWQNYELEKAKRHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQRALLAVFGK
                     VLADNVKIEQVRIFMDKRGIESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFT
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSGTCEYVINTTKGGKVIAKTLNNWWNQAKHAA
                     EKKSGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(1119..1370)
                     /locus_tag="ACHOQQ_RS11705"
                     /old_locus_tag="pgaptmp_002341"
     CDS             complement(1119..1370)
                     /locus_tag="ACHOQQ_RS11705"
                     /old_locus_tag="pgaptmp_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU4470581.1"
                     /translation="MYELTLSPAEIQEITRYERYTKQQQQLRLHGIPFVPGPKNEPIV
                     LRSDVPRGLTAIPKVSELVSADPDFEALNNGKTKKKQKR"
     gene            complement(1437..3875)
                     /locus_tag="ACHOQQ_RS11710"
                     /old_locus_tag="pgaptmp_002342"
     CDS             complement(1437..3875)
                     /locus_tag="ACHOQQ_RS11710"
                     /old_locus_tag="pgaptmp_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4470582.1"
                     /translation="MSTDKEEFSLYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTLDTLD
                     VEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINGGLA
                     AKCKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTAEEV
                     NKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQNQQETEQNG
                     QKAEQNSPNALQNEPETKQPEPVVQQEEEKVCTACNQTGGGNCPDCGAVMGDATYQET
                     FNEESQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETGETANPLIKVNGHREITS
                     TSRLWHHLMIDLETMGKNPDAPIASIGAVFFDPQTGEQGPEFSKIIDMGTCGGTVDIS
                     TIEWWLQRSGEARAAILADRIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVILR
                     RSYERQEIPCPWRYTNDRDVRTIVALGLVMDFDARSVITFEGERHNALHDARYQAKYV
                     SAIWQKLFPNQADF"
     gene            complement(3968..4156)
                     /locus_tag="ACHOQQ_RS11715"
                     /old_locus_tag="pgaptmp_002343"
     CDS             complement(3968..4156)
                     /locus_tag="ACHOQQ_RS11715"
                     /old_locus_tag="pgaptmp_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4470583.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(4153..4341)
                     /gene="dicB"
                     /locus_tag="ACHOQQ_RS11720"
                     /old_locus_tag="pgaptmp_002344"
     CDS             complement(4153..4341)
                     /gene="dicB"
                     /locus_tag="ACHOQQ_RS11720"
                     /old_locus_tag="pgaptmp_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4470584.1"
                     /translation="METLLPNVNTSEGCFDIGILLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(4352..5206)
                     /locus_tag="ACHOQQ_RS11725"
                     /old_locus_tag="pgaptmp_002345"
     CDS             complement(4352..5206)
                     /locus_tag="ACHOQQ_RS11725"
                     /old_locus_tag="pgaptmp_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4470585.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGRGNPMMTTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPKGWPVSVVTGISTPVRLTTLMVVESLGGELFKLTIEAAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKM
                     IHGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTV
                     EQLLKIHC"
     gene            complement(5725..5877)
                     /locus_tag="ACHOQQ_RS11730"
                     /old_locus_tag="pgaptmp_002346"
     CDS             complement(5725..5877)
                     /locus_tag="ACHOQQ_RS11730"
                     /old_locus_tag="pgaptmp_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4470586.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            6152..6454
                     /locus_tag="ACHOQQ_RS11735"
                     /old_locus_tag="pgaptmp_002347"
     CDS             6152..6454
                     /locus_tag="ACHOQQ_RS11735"
                     /old_locus_tag="pgaptmp_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HGU4470587.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            6457..6816
                     /locus_tag="ACHOQQ_RS11740"
                     /old_locus_tag="pgaptmp_002348"
     CDS             6457..6816
                     /locus_tag="ACHOQQ_RS11740"
                     /old_locus_tag="pgaptmp_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4470588.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(6863..7255)
                     /locus_tag="ACHOQQ_RS11745"
                     /old_locus_tag="pgaptmp_002349"
     CDS             complement(6863..7255)
                     /locus_tag="ACHOQQ_RS11745"
                     /old_locus_tag="pgaptmp_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4470589.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            7382..7642
                     /locus_tag="ACHOQQ_RS11750"
                     /old_locus_tag="pgaptmp_002350"
     CDS             7382..7642
                     /locus_tag="ACHOQQ_RS11750"
                     /old_locus_tag="pgaptmp_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4470590.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANEQAAAGVDS"
     gene            7639..8076
                     /locus_tag="ACHOQQ_RS11755"
                     /old_locus_tag="pgaptmp_002351"
     CDS             7639..8076
                     /locus_tag="ACHOQQ_RS11755"
                     /old_locus_tag="pgaptmp_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4470591.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            8163..9173
                     /locus_tag="ACHOQQ_RS11760"
                     /old_locus_tag="pgaptmp_002352"
     CDS             8163..9173
                     /locus_tag="ACHOQQ_RS11760"
                     /old_locus_tag="pgaptmp_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HGU4470592.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAERKVFTQVQWEQKFARHVQHVRAQVKPVSKGGSHAASGGTAS
                     RAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            9166..9627
                     /locus_tag="ACHOQQ_RS11765"
                     /old_locus_tag="pgaptmp_002353"
     CDS             9166..9627
                     /locus_tag="ACHOQQ_RS11765"
                     /old_locus_tag="pgaptmp_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4470593.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKYPKGPTPIEWLKQEYERRKAAGFI"
     gene            9661..10422
                     /locus_tag="ACHOQQ_RS11770"
                     /old_locus_tag="pgaptmp_002354"
     CDS             9661..10422
                     /locus_tag="ACHOQQ_RS11770"
                     /old_locus_tag="pgaptmp_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4470594.1"
                     /translation="METVFDALKAMGKASSVGLAERLDISREEVLNELWELKRNGVVD
                     KAGHIWFLAGEGESGVTEEQPEQSEVPDVLTGEVEQKVTADMMIEFIGQEGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAVPVTENDGKA
                     FPQPAGVALPVQESATQEDIKTETVADIVQSLPSFTETQADELIFLSLCRANLALRRA
                     KSDVQKWERVCAALRELNKHRDILRDITATRELLR"
     gene            10455..10736
                     /locus_tag="ACHOQQ_RS11775"
                     /old_locus_tag="pgaptmp_002355"
     CDS             10455..10736
                     /locus_tag="ACHOQQ_RS11775"
                     /old_locus_tag="pgaptmp_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4470595.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            10733..11038
                     /locus_tag="ACHOQQ_RS11780"
                     /old_locus_tag="pgaptmp_002356"
     CDS             10733..11038
                     /locus_tag="ACHOQQ_RS11780"
                     /old_locus_tag="pgaptmp_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4470596.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            11025..11675
                     /locus_tag="ACHOQQ_RS11785"
                     /old_locus_tag="pgaptmp_002357"
     CDS             11025..11675
                     /locus_tag="ACHOQQ_RS11785"
                     /old_locus_tag="pgaptmp_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4470597.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLSEVRALGVDA
                     AIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLIGKVEMTEWLVDFAAQLRKGVQS"
     gene            11672..12001
                     /locus_tag="ACHOQQ_RS11790"
                     /old_locus_tag="pgaptmp_002358"
     CDS             11672..12001
                     /locus_tag="ACHOQQ_RS11790"
                     /old_locus_tag="pgaptmp_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470598.1"
                     /translation="MRASISYVDDCHLSVRVDEIVSSVPTFPTKNAAVNAGAPFGWRT
                     AVRIERRFENVWVVGKKCFQSDRSAGLNFEAYRFPFLRWEKEGGITKCPILSVRRFKQ
                     EATSEQD"
     gene            11988..12332
                     /locus_tag="ACHOQQ_RS11795"
                     /old_locus_tag="pgaptmp_002359"
     CDS             11988..12332
                     /locus_tag="ACHOQQ_RS11795"
                     /old_locus_tag="pgaptmp_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4470599.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVGIIADLLDELEEAKQRIDEQESRIVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            12334..12687
                     /locus_tag="ACHOQQ_RS11800"
                     /old_locus_tag="pgaptmp_002360"
     CDS             12334..12687
                     /locus_tag="ACHOQQ_RS11800"
                     /old_locus_tag="pgaptmp_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470600.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAKP
                     VASCIIEDGGMCVDGFGEYVGHSLPDGTHQLYAAPPVPSKLPREYRNGWPLAYSDYAE
                     GWNDCREAMLQGDKS"
     gene            12684..13046
                     /locus_tag="ACHOQQ_RS11805"
                     /old_locus_tag="pgaptmp_002361"
     CDS             12684..13046
                     /locus_tag="ACHOQQ_RS11805"
                     /old_locus_tag="pgaptmp_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4470601.1"
                     /translation="MINRIKLEHILEYARQQKHTGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPDTKTAVLVAREFDRKGDWRMKWATYIPGHPDANDGWVVPGAS
                     WIPSHWMPLPEPPKEVRQ"
     gene            complement(13249..13548)
                     /locus_tag="ACHOQQ_RS11810"
                     /old_locus_tag="pgaptmp_002362"
     CDS             complement(13249..13548)
                     /locus_tag="ACHOQQ_RS11810"
                     /old_locus_tag="pgaptmp_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4470602.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNIIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGRKVFIVGIYHGGQSYETDFL"
     gene            complement(13554..13811)
                     /locus_tag="ACHOQQ_RS11815"
                     /old_locus_tag="pgaptmp_002363"
     CDS             complement(13554..13811)
                     /locus_tag="ACHOQQ_RS11815"
                     /old_locus_tag="pgaptmp_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4470603.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRVAIRSGK"
     gene            13947..14225
                     /locus_tag="ACHOQQ_RS11820"
                     /old_locus_tag="pgaptmp_002364"
     CDS             13947..14225
                     /locus_tag="ACHOQQ_RS11820"
                     /old_locus_tag="pgaptmp_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470604.1"
                     /translation="MAQDTKLHNSDNSTAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            14227..15285
                     /locus_tag="ACHOQQ_RS11825"
                     /old_locus_tag="pgaptmp_002365"
     CDS             14227..15285
                     /locus_tag="ACHOQQ_RS11825"
                     /old_locus_tag="pgaptmp_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4470605.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISSTQERE
                     LSLAELSWWAVCNQVVDALPEAVVRRSLGLPAEKIRSVYRESDIIPGEQAATSILKQR
                     TKNIALPLHVHQQQNPPQEKTLVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            15286..15666
                     /locus_tag="ACHOQQ_RS11830"
                     /old_locus_tag="pgaptmp_002366"
     CDS             15286..15666
                     /locus_tag="ACHOQQ_RS11830"
                     /old_locus_tag="pgaptmp_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4470606.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVR
                     GKPVSGGRLGVKIYPIMLEGQVKK"
     gene            15663..16484
                     /locus_tag="ACHOQQ_RS11835"
                     /old_locus_tag="pgaptmp_002367"
     CDS             15663..16484
                     /locus_tag="ACHOQQ_RS11835"
                     /old_locus_tag="pgaptmp_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4470607.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDCVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCSCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVSKFDIEEAWAERQLKKVTR"
     gene            16711..>16862
                     /locus_tag="ACHOQQ_RS11840"
                     /old_locus_tag="pgaptmp_002368"
     CDS             16711..>16862
                     /locus_tag="ACHOQQ_RS11840"
                     /old_locus_tag="pgaptmp_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4470608.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNMYLSRERCQLIL
                     TQLVMAG"
BASE COUNT         4320 a         3818 c         4410 g         4314 t
ORIGIN      
        1 ataaaaatca atgcaatact aaaaacagct gaaaaattaa ccaaacttaa cacctagaaa
       61 aatcaggctg ttagggagct ttcagagtta gtataccaag tcttttttgc cagaaaattt
      121 cctgagttgc ctacttgctg accacaagtg gcaaatccag tgtcggcgtg atttttgttt
      181 tacgatcata aatcaacacc tgattttctg ttttatgtcc actgaaaatc tgtttgtcgc
      241 gactgcttcc ttcgtaatct gatattcctt tggcttttat gtcatggaaa ttgcacccaa
      301 acgggacgcc ggattttttc tcggctgcgt gtttagcctg gttccaccag ttgtttagcg
      361 ttttagctat gaccttcccg cccttggttg tgttgatcac atattcacat gtgcctgaag
      421 atacatttct ggctaactgg atcgcggttc gtaatcgtgg agaccattcc ttgatttgtt
      481 tggtgccggt cttgttttgc tcaatgtaga ttcctttatc cataatatcc tgccatttca
      541 gctcgagcac atcaccgagt cttgccgcac aaagatagga tatctccatt gcaatgcgta
      601 actgtggtat tgcttccgca tatatcgccg catactgttc atctgtgatg taaacagtgc
      661 gggctttaag cgtaaatttc ctgactcctt tgcatgggtt attcttcaca tatccacgct
      721 catatcccca cccgtataca cgactgagac ttgccagttc atggtttgcc tgggtcttgc
      781 tctcaatccc ccgtttatcc atgaaaattc ttacctgctc aatttttaca ttatcagcca
      841 gtacttttcc gaagactgcc agtaacgccc tctgatgttg ccgataatct ttttgggttc
      901 tgggggccag ttctgtaaat gcgggggaat ccataaacat gtgccataat ttagcgacgg
      961 tcattatgtt gtggcgtttt gctttttcca gttcataatt ctgccagact ttagctatgc
     1021 tggtttcccg cactcttcct agccctatag tttttgtgct tccttcgggt ttccatacgt
     1081 aactgtaacc attcgatcta acacgtggcg gtagtgcatt atcttttttg ttttttcttg
     1141 gtcttcccat tgttcagcgc ctcaaaatcg gggtcagcag aaaccagttc agatactttc
     1201 ggtatcgcag tcagtccgcg cgggacatca ctgcgaagaa ctattggttc gtttttagga
     1261 ccgggtacaa atgggatacc gtgcaacctt aactgttgtt gctgttttgt ataccgttcg
     1321 tatctcgtga tctcttgaat ttctgctggc gatagagtta attcgtacat gtggtcacgt
     1381 gccttacagc atgaccgccg ccactatagc ttgtgggcgg cgatatgggt tgaacattaa
     1441 aaatcagcct gattcgggaa cagtttttgc cagatagctg aaacgtattt tgcctggtaa
     1501 cgcgcatcgt gcagggcatt atggcgttca ccctcaaatg tgatgacact tcgagcatcg
     1561 aaatccatca ccagccccag ggcaacaatc gttcttacat cacgatcatt ggtgtaacgc
     1621 cacgggcagg ggatctcctg ccgttcatat gaacggcgta aaatcacgtt gtcgaagttg
     1681 gctccatttc cccagacctg aacaaaaaac tcaccggagt tttcgtctat aaattcccgt
     1741 aattgtaaaa gcgcatcatc aagcggtatt cgatcagcta aaatggcggc acgggcttcg
     1801 ccagagcgtt gaagccacca ttcgatggtg cttatgtcta cagtgccgcc acatgtaccc
     1861 atatcaatta ttttgctgaa ttcaggcccc tgctctccgg tttgtggatc gaaaaataca
     1921 gcgcctattg aggctattgg tgcgtcagga tttttgccca ttgtttcaag gtcaatcatc
     1981 agatggtgcc acaacctgct ggtggatgtg atttcacgat gaccgttcac cttaattaag
     2041 ggatttgctg tctcgcctgt ttcattattg ccattggcat gttgatcgcc ttcggtgcac
     2101 tccttgttcg ggagtccggc actttccatt tcctccggat ctttttcccg ggcttcatcc
     2161 tggctttctt cgttgaatgt ctcctggtag gttgcgtccc ccatcactgc gccacaatca
     2221 gggcagttac caccaccagt ctgattgcat gcggtacaaa ctttttcctc ttcctgttgc
     2281 actactggtt caggttgttt cgtttctggc tcgttttgta acgcatttgg gctgttttgt
     2341 tccgctttct gaccgttctg ttccgtttct tgctggttct ggttcacaga atcgcaggtt
     2401 tcaatcccct taacccattt cgaatcattc gggtcgctaa tcccttcaac aaactcacca
     2461 cgagaagcgg caagcaattt gtcggcatct ggtcgactga tgtctgctgc ctccataatt
     2521 ttatttactt cttcagcagt aacttttacc tgtgacttgt catccagcgg ctgcgggtcc
     2581 tgatgatgtt caactgtatc cgctgccatt gtttcagccg ttgcctgttc atctgccatt
     2641 gcgccagatg gttgcgggtt ttcttcatca tttttttctt cttctgtttt gcacttagca
     2701 gccagtccgc cgttaatttc ttccaggata tctttttccg gtgtatgccg ggcagccatg
     2761 agcgtttcag ctgtgggatt ctcgtgatca gtttctgtca ggttggcatt gatatacccc
     2821 tgaaggcgtc ccgggtagtg ataaaactca gggtgggcgc tccggataag tgcaaaaata
     2881 gctgcgcggg aatagtccag gataccaggg gttgcgcgaa gtgctgcgga ccattccctg
     2941 aacggacttt cttttttcag gacgatttct tttgcgcgac gataaacgct cacgggtatt
     3001 tcataaatat taaaatccat cggaagtgtg gctgctgcag cctccacatc cagagtatcg
     3061 agggtgtgta ctaagtcagg attacgatcg gttttatttc cgccaccggc atttgcaccg
     3121 gaagccgtgc gggtgatgcg agaaacacga tttcctttca tccactcttt tgtcagcaga
     3181 cctcgatcgg tatagtcagc gtccaggtat gcttcgaaaa aagcagtcat taaccccaga
     3241 ctggaattaa cgggattagg gaaaactttg tctgtgtcgc gtaccagttt gtgaaggtcg
     3301 cgaatctcca gcgggtcgag cagctttgtg ttgtgggaaa tagccagggc agtaacagcc
     3361 ggcagttctt cagctcgtgc gatatgtaat gcctgaagtt cttcccgtga aacgtgcgtt
     3421 accggttttt cgctgccatg ttgcgcaagc cagcgaatgg gcagttcctg accggaaatc
     3481 ggcaggagca tattatcctc aatctcagcc atgtcttcgc cattgacgtt ggtattgtca
     3541 gtgctggctg gtttgtcctg aactgagggg gaaggcgcta taaatatcat tgtgatgcca
     3601 tcttccccgc ctttttcata gcggttgcag aattcggtat caaacacgcc ttccggtgga
     3661 aggtcattca caacggggaa atggacgcgg acaggttttt tgaaatcatc agcgtcgtag
     3721 cctgcctcgt ccattgcaac aacgcaccgt gagatggcaa cagataattt ttttgctgta
     3781 gtccagtaaa aacctccttt aatcccaagg cgttttctga ccttgtcatt ttttgcttcg
     3841 caatatagcg aaaattcttc tttatcagtg ctcatggata aacctcataa ctattttaag
     3901 gttgtacgaa cccctgccat tgctggcatg cttaatcaac gggtatggcg ttaatatggc
     3961 tggtggatta tccagccggt atttcgttat tcaggtacag cgatactttt tttaacggga
     4021 ggcattcgcc agaaattttt tgctcgtctc ttgcacggag gcatgattct ttactggcat
     4081 aaattccggt aatcacattc tgtggctcac ccgttataag aaaaaccgtc atcaccagtg
     4141 caaatgcaga agtcactgct gccctccgaa aataccaagt ttaagaaggg caattctgga
     4201 gagtatggaa ttatcattga gaagataagg ctcatatttt ctcatcttaa tggcatcttc
     4261 agtaaactcc cggttactga gcagaatacc aatatcaaaa cacccttcag acgtattaac
     4321 gtttggtaat aacgtttcca ttatcgcgtc ctcaacaatg aatttttagt aattgctcca
     4381 cagtcatatt tttaattgcg ctccggttta caagagtcca gccttgtttc tccagataaa
     4441 actggaaggt atccagggtg cagaccattg caccatcagg aacggtttca gtgaatttga
     4501 tattgccgtg ttcgtcgaga cggataacca gggtacgtcc gtccccgtga atcattttgt
     4561 cgggagacgg ggcgttattc tggcgcagtt ctttctccat gcgatcgaac tcagcgatgt
     4621 aggcttcttt aaatgcagcg gcttttttgc cggtgaagcc catcaccagg aaaacgaagc
     4681 cgtttttggt gatttggtac attgggagtt tgcgaccggt tgagtcggta tattcgctcg
     4741 acacaaaatt gtgctcagtg aattttgctg aacagtccag attgcgaatt ttatccaaca
     4801 ctcgttcgtg gcgtttacca aagaactcag cgatcgcaat agacgtagtg acagcgcgtc
     4861 cattttcgat ggttacgtca gggtgagaaa gggcagggat agtagccatg atggcagcct
     4921 cgatagtcag tttaaataac tcaccaccaa ggctttccac gaccataagg gtggtgagac
     4981 gtacaggggt ggaaataccg gtgactaccg aaaccggcca gcctttcggc tgccctgcac
     5041 gccccaccat aatctgaatg tggctgtgca ttacgcataa aaaaaccgcc tgagcgcggt
     5101 tatgcgcagt agtcatcatc gggtttccac gcccggcacc cgctttatga ggtgcaggtg
     5161 cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc tcttaccctt
     5221 catcactgaa gtgaactttg ttgatgcagt gcctggtgcc tccaggtgac gttaaccagt
     5281 taacaattaa cgccggaata cagaaggatg cccgttacgc cccgtaaaag accactttac
     5341 ggttttaact gttccgcgtg cgcttagccg cattcaccgc atcacaaaat tcactttaaa
     5401 aagggcggac atcagaaagc taataaactg atgccgccaa aggtaccact gccattgaaa
     5461 catggagatg ttgtggcggg gttgtcactc aggcatatgg tcaacctgac aacccggtgt
     5521 cctcaatgag gggaaaagaa ataaaaaccc ccagccatat ttaccgccgc gccatttcgc
     5581 ggattgccac aaccggaagc acacggtcga agaaatctaa cgacaagcct tctaaggaaa
     5641 agacgccttc gccgtgtgct ttctgttgtg ccctgacttt tcagggtaaa ttaaccagtg
     5701 gcaacctgtt tttactggtg ctaatcagtt agtgtttctg gctaaccagc gacgcgcgcc
     5761 agcttcggtt ttaaactttt tacttttggt atacgtcatc gcggtaaacg tgccgtcctg
     5821 gttagggaac acaccgcata ccagagattc gttgttgcca agattgagcg tatccatgtt
     5881 gacctcattt ccccttaacg ccggggtagc ggaacaaaaa acctgctgca tagttaaacg
     5941 taatccccgc cgtcatgttc atacgcctca ggctggctac ttaacccctg accactgccg
     6001 ggtaactcga agtattgccc tgcgttctgt ggggcggggt gggtttatgg atacaattta
     6061 caaattaaaa tttaactggt caacatgttt attattaaat ttgtaattgc gagctgttgc
     6121 atcagtccca aaatgggact actatacagt tatgaagatt atctcagtta aaacactcag
     6181 ggatttttgg gcggagaatc ctgatgcaga acaaccgcta agggcatggg tggatgaggc
     6241 ggcaaaagct gactggaaaa gcccagcaga cattaaggca caataccgaa cggccagtat
     6301 attgaaaaac cggcgcgtgg tattcaacat aaaaggtaat cattaccgtt taattgttgc
     6361 aattgcgtat cagcgaggat gggtatttgt taaattcatc ggaaaccaca aggaatacga
     6421 tgctattgat gctgatacca tcgaactgga gtaagcatga acatcaaacc tattcgtaca
     6481 gagcaagatt atgaagccgc gttgcgtgct gttgaaccca tgttcgacaa tgagcccgaa
     6541 attgacacgc ctgaggggga tttttttgag gtgatgtgtt tgctaataga ggaatatgaa
     6601 aaaaaacatt atcccattga gccaccatcc ccaattgaag ctataagatt tcgcatggaa
     6661 cagcaggggc tgactgtgaa agatttggaa cccgcaattg ggaaaaaaaa tcgggtttat
     6721 gaggtgttga atggtaccag aagccttacg ttaccaatga ttcgccgtct tcataatcaa
     6781 tttggtatcc ccctggaaag cctggttgga ttataaaatc tgctagtcat ttgcctgatg
     6841 ctcgttccag aaaaggaatg catcaggcag ttttgttttt ctgccgcagt aactcttcaa
     6901 gtttccgttt atagaaatcg cgtttttgct ccatatcacg aatgatctgc tctgcgtcgc
     6961 tttgaggtaa ctcatctaaa agcgatatga tttttcgttg ttgttctgta agttgcggtt
     7021 ggttgtcatt actggataca gccattttat cgccgagagt ttcttcttcc ataaagaacc
     7081 aatggacggg atgttgtgag agctctgcta atttttccag tttatccatt cttggcatca
     7141 cgcctttcaa ccaaccttgc acggattggg gttttacacc aagacgtctt cccagctctg
     7201 actggtttat attcaattcc tgcaacacct gctgaaggcg ttttacaaag atcatcacca
     7261 cccctcgtaa actagttccg cgatcctaca gaaaaaattg ataagtggca ttacaaatag
     7321 aagttgaaat ttaaatttaa atttgtaatc atcggtgtca tcgtaaagtt cggagggaaa
     7381 catgcaaaaa agtactcaag tgaaaatcct gtcaataatg agccaatcag aattagggcg
     7441 tcgtcttggt aaaacaccgc aaaccataag tgggtggttt aaaaaacgag tgccagcgga
     7501 ggaggttata ccagcatgtg aggcgcttga ctggggagta actccgcatg aattgcgccc
     7561 tgataaatac cctaatccaa ccgatggttt acctgttgag tatcaggcta acgaacaagc
     7621 agcggcggga gttgattcat gaaaatcaag catgagcaca tccgcatggc gatgaatgcg
     7681 tggctgcttt atccgagggt agggcgcaaa aaaatcgctg atgatatagc gacagcatat
     7741 tttgagcttg aaatgactta tccaccaatg catgacacct ctacgacaga gggtattgga
     7801 ttgaatatac aaaatatttt tcgctggctt gaaaaggata cgcctgatgc tgttgaaaaa
     7861 attcaggcac taattccggc tatcctgact gttcttccgc gtgaactgcg ttatcacctc
     7921 agtatttttg acactgttga gcgccgtgca ttactggcgg cgcaggaagc gttgagcacg
     7981 gcaattgatg cacatgatga tgctgtccag gccgtttacc ggaaagcgta tttcagcgac
     8041 ggcgggtcat ccggcgagtc tgttgtggtg cattgatatt tatgccagcc ccctgctgat
     8101 tctgttgatt ggggaatcac agaatatcac cagaggatgg ttcgtcacaa gatgaggcaa
     8161 ttatggccgc attaccatac atgcaactgt acatagctga ttacctagct gacaccatgc
     8221 atttgtccgc agaggagcac ggtgcgtatt tgttgctgat gttcaattac tggcaaacag
     8281 ggaaaccaat acctaaaaac aggctggcaa aaatttcccg tctgactaac gagcgatggg
     8341 ctgatgtgga accatccttg cgggagtttt tttgcgataa cggcgatgaa tgggtgcatc
     8401 ttcggattga ggaagatctg gcatcagtca gggaaaaatt aaccaaaaaa tcagccgccg
     8461 gaaaagcatc tgttcaggcc agaagaagca gaaaggaagc atatgttcaa acaaaacaag
     8521 agagagattt aacaggcgtt caaacaaatg ttggtgttgt gtttgaacat gacgcaaaca
     8581 caaaagcaac taataaagat acagatctaa aagaattaaa ccccacacat aacgtgcgtg
     8641 aacgcgagag tattccgacc agtgagtcgc atggtgcgcc gttgcagaca gccgaacctg
     8701 aatacctgga cggcctgagc gaaccgatcg ggaaattttc gatgaccact gtctggcagc
     8761 cgtcgccgga ttttcgacaa cgggcagcag tgtggggtat ggctctgcct gagccggaat
     8821 ttacacctgc tgagcttgcc gcattccggg attactggat ggcggagagg aaggttttca
     8881 cacaggttca gtgggagcag aaatttgccc gccacgtgca gcacgtcagg gcacaggtaa
     8941 aaccagtcag caagggggga agccatgcag catcaggtgg cacggcatca cgggcagttc
     9001 aggaaatccg ggcagcacgc gaacagtggg aacgagacaa cggatttatc agcaacggaa
     9061 acggcctgga agctgtggga gcttatgggg gaggtgtatt cgaaccgctg gactcagaag
     9121 aacggggccg caccttcgaa gctctggatt gcccagattg gtgcgatgac tgaacagcaa
     9181 atccggcagg tctgccgtca gtgcatggac cgctgccggg cgggtgaaac gtggcccccg
     9241 gacctggctg agtttgttgc actgatttcg gagagtgggg caaatccatt tggtcttacg
     9301 gtggatgcag tgatggaaga gtaccggcgc tggcgcaatg aatcctggcg atacgacggg
     9361 agtgataaat acccgtggcc acagcctgtg ctgtaccaca tctgcctcga aatgcgtacc
     9421 agagggattg agcgccagat gacgcagggt gagttaaaac gacttgcgga acggcaactg
     9481 acgaaatggg caaagcatgt tggtaacggg atgagtgttc cgccagtgcg acgacaactg
     9541 gaaggggcga aatacccgaa agggccaacg ccaattgaat ggctgaaaca ggaatacgaa
     9601 cgccggaagg cagctggttt tatttgaatc tgagaagcga ttttgtagga ggaaatttta
     9661 atggaaaccg tttttgacgc actgaaagca atgggaaaag ccagctctgt tggactggct
     9721 gagcggcttg atatcagtcg tgaagaggtt ctcaacgaac tgtgggaact caaaagaaat
     9781 ggcgttgttg ataaagcggg tcacatctgg tttctggctg gcgaaggtga atccggggta
     9841 accgaagagc agccagaaca gtctgaagta ccggatgtgc tgaccgggga ggtcgaacaa
     9901 aaagttaccg cagacatgat gattgagttt atcggtcagg agggggctaa aacgtgtgag
     9961 gaactggcgg ataagttcgg tgttagcatt cgcaaggttg cttccacgtt ggcggtggta
    10021 accgcaacgg gacgcctggc acgcgtaaat cagaacggta aatttcgtta ctgcatgccg
    10081 ggcgataatt taccagcaga gccgaaagcc gtgccggtaa cggaaaatga tggtaaggcc
    10141 tttcctcagc cagcaggtgt tgcgttacca gtacaggaat ctgcaacaca ggaagatatt
    10201 aaaacagaaa cagtggcgga cattgtgcag tcgttgccat cgtttaccga aacgcaagca
    10261 gatgagctga tttttctgtc cctgtgcagg gcaaacctgg cgctgcgcag ggcgaaaagt
    10321 gatgttcaga agtgggagcg agtctgtgcc gcgctgcggg aactgaacaa acacagggat
    10381 attctccggg atattaccgc caccagagag ctgctgcggt gagtggctgg aagaagtggc
    10441 gctgggcgga aattatgata ctccggcagt gtgcgggaac gatgacagtc gaaagcatcg
    10501 gcagtctgat tggccgtagt ggggctgccg tcaggacgaa agcgcgggaa ctgggtatct
    10561 gcatgatgtt acggggtgat tatcaccagt cagccaaatg ttcacagcgt gatattgagc
    10621 tggcgcggca actgcatcag cgtggcgtac cccgacggga aattgccgaa aagtttggga
    10681 tgaagttggg cgcagtgaat aactacgttt atttcgacag gagggttcag gcgtgagggt
    10741 gagaatttat atcgccggtc cgatgacggg atatgaaaat ttcaaccgtg aggcgtttca
    10801 cagggcggaa gaagcactga aacgggaagg gcataccgtt ttaaacccgg cagtacttcc
    10861 ggacggactg acacagccac actacatgga tatttgcatg gcgatgatac gctgcgtgga
    10921 tgcgatttac atgctgaaag gctggcagcg gtcggcaggc gctaaggcag aactggcact
    10981 ggcggagaaa ctgggacatg cggttatttt ccaggaggca accagtgaga aaaattaatt
    11041 atcagacgct acgtgagata gcaaaactgg caacccaggg cgaatgggtc gcattcattt
    11101 cgccgggcac tgatacatat gcggtgcata cgccggggga tgaacgttgc ggagacatta
    11161 tcaaatggcc tggctttgat gaccagaaaa atgcagagaa caacgcagaa tttattgcag
    11221 ctttcaaccc tgaagtagta caggcactgc tggatgaacg ggaagcccaa agcaaacgta
    11281 ttgcagagct ggaagctaat cttgtggcgc tggcggcgga gaatgcactt gctcgtaaag
    11341 cagttcaggc attctgcgat gttgttggcg acaacaccga ggttatctgc gaggaggttg
    11401 ggagagatgg cgttctggtt attttggagg ccatgaaggc aacaggaaat atgccagcca
    11461 ccgatgcttt cctgtctgaa gtgcgggcgc tgggggtgga tgctgctata gaagctgcaa
    11521 aaaatctggt ggcccaagaa tatgagtata aggatttcaa agcggcgcag agtgattgct
    11581 gtatgcaccc tggttcagac ctgataggga aggttgaaat gactgagtgg ttagttgact
    11641 ttgctgccca gcttcgcaaa ggagttcagt catgagagct tcaattagtt atgtcgacga
    11701 ttgccaccta agcgtgcgtg ttgatgaaat tgtgtcatcc gtgccaactt ttccgacaaa
    11761 aaatgcagcc gttaatgcag gcgcgccatt cggatggagg acggcagttc gtattgagcg
    11821 tcgattcgaa aacgtttggg tggtaggtaa gaaatgcttc caaagcgatc gctctgcggg
    11881 gcttaatttt gaagcatatc gattcccgtt cctccgatgg gagaaagagg gcgggattac
    11941 aaaatgcccg attctttccg tgcgcagatt caagcaggag gcaaccagtg agcaagattg
    12001 actatcaggc attgcgtgag gcagcacaaa actatcggtc gatgctggcg tggtatcagg
    12061 agaaaccaga cagtccaaac gctgagcagg attgtgatgc agctttggct gcgtttaagc
    12121 gtgaaatccg tcatcgtgaa gtgggtatta tcgctgattt gctggatgaa ctggaggaag
    12181 caaaacagcg tatcgacgag caggagtccc gcatagtgaa gttaccagaa ccattcaagc
    12241 tggctaaatc atcaagtgga ttaacgtact actacgctga tgaggtcaat gccgcactga
    12301 ctgctgctgg tattcgcata gaaggagagt gagatgacca ctattaccaa agagcgactg
    12361 caatggctgg ctaacatttc tggccgcgat gacattgacg atatagacgg cggtgaaatt
    12421 cgtgagctgg cgcttatcgc gctggcatcg ctggaagcga agcctgtagc ttcatgtatt
    12481 attgaagatg ggggcatgtg tgttgacggg ttcggtgagt atgttggtca ctcgctgcct
    12541 gatggaacgc accagcttta cgccgccccg ccagtgccta gtaagttgcc gcgtgaatac
    12601 agaaacggtt ggcctcttgc atatagtgat tatgctgaag gctggaacga ctgccgcgaa
    12661 gccatgcttc agggagataa atcatgatta atcgtatcaa gctggagcac atcctcgaat
    12721 atgccaggca gcagaagcat actggtcagc attgtaaaat tccaccaggg gatatggttg
    12781 aaatcatgga gattgccatg cgcaaggctg gcaattctcc ggtaactccg gatggttgga
    12841 taagctgtag tgagcgaatg ccggatacca aaacagccgt tcttgttgcc agggagtttg
    12901 acaggaaagg tgactggcga atgaaatggg cgacttacat cccggggcat cctgacgcta
    12961 atgatgggtg ggtagtacct ggtgcgtcgt ggataccatc acactggatg ccgctaccag
    13021 aaccgccgaa ggaggtgcgc caatgagctg gcctgaggcc ttctcaaatg ttggtattgc
    13081 aatggcggtg gcgctggtgg ggtattcgat ttgccgctgg ggataaatcg ccgaaaaaag
    13141 atcccgccac aaacatgagc cgggatcttt gatttatata gcctacgaat ccgcagtaag
    13201 agaggggcag acggtttatt ctaacaccgg aatgatgtgg gtaaaagttt ataagaaatc
    13261 ggtttcataa ctttgcccac catgatagat accgacaata aagacttttc tgccatcaac
    13321 ggcaaaagca ataatcgttc tgtggcggaa atgagttacc cgcatcccct ggcgaatatc
    13381 atcgcgttta ttgccccgat gcgggaatgt agaaaaccca tcaagataat caagaagcgc
    13441 attggcataa ttgtcagcaa tgatgttccc tgctttctcc gttatatatc tgtgcaggtt
    13501 gattatttgt tgttcggcct caggagtaat gatgacttca tatgtcatgc agattacttc
    13561 ccggatcgaa tcgcgacgcg aacctgtgaa atggagcgtc cgttgtttgg gttttcgcgg
    13621 atagaatcaa gagagggggc ggctgaatgc gttaaccacg cttcgattgc tttatcgcgc
    13681 tcattcagtg cgcgaagccc ttcacgaatg acctcgcttt ctgaagcata ggcgccggaa
    13741 gccacacggg cgcgcaccat gtcagccatc tcgttagtta atgtaatgct gaattgttgg
    13801 gttgtacgca tggtaaacct cacggagtag gatagaacac tattcgatga tagcacgctg
    13861 cctgttgacg acaacagaaa tcagagacaa tattgccgca cgccagcctg aacaactggc
    13921 acctgctgcg ccagcagaga caaccgatgg cgcaagatac caaattacac aattcggata
    13981 actccaccgc ccctgccagc aggcacgggc ggcgttctca tgcattcaaa tctgactggt
    14041 tccagcatga cccatgcact gaagaacagg ccgaatggct gatccagtgc taccgcagac
    14101 gtggttacga gtttcagaaa gatctcagct tcgatcgtcg tcactggata atctccgtca
    14161 ggctccctta ttccgaacgc ccaccgcgtc cgtcccgcac attccagcag cgcatctgga
    14221 ggtaacgtgc gggtattact tcgacctgtt ctggtaccgg aactcgggat ggtgctcctt
    14281 aagccgggcc gtgaatcaat gtcagcattc cataacggca gaatattggt ggaaccggaa
    14341 ccgaaaaaca tgcgcggtct gccgtccgga gtcgttcctg ccgttcgcca gccgctggca
    14401 gaggataaaa cattactgcc atttttcagc gatgagcggg ttattcgtgc agcaggtggt
    14461 gcaggtgcac tgtctgactg gttattacgt cacgtgaaat cctgccagtg gccacacggt
    14521 gattatcatc acagcgaaac cgtcattcac cgttacggta ccggcgcgat ggtgttgtgc
    14581 tggcactgtg acaaccagct gcgcgaccag acatcagaat cactcgatca acttgctcag
    14641 cagaatctgg ttgcctggat gattgacgtc atccgtcacg caataagcag tacgcaggag
    14701 agggagttat cgctggccga attatcctgg tgggcggtct gcaatcaggt ggtggatgca
    14761 ctacctgagg cagtagtgcg tcgttcgctg ggattaccag cggaaaaaat ccgctccgta
    14821 taccgtgaga gcgacatcat accgggagag caggccgcca ccagcatact gaagcagcgc
    14881 acaaaaaata ttgcgctgcc acttcacgtc caccagcaac aaaatccccc ccaggaaaag
    14941 acgttagtaa gcatcgccgt tgatccggag tctccggctc agtacctcca gcgccagaaa
    15001 ccacaacggg aagagatgcc tgtatacacg cgctgggtaa aaacgcagaa atgcatgacg
    15061 tgtggcaatc aggcagatga tccgcatcac atcattggtc atggactggg aggtatggga
    15121 acaaaggctg acgatttgtt tgttattccg ttatgccgta aatgccataa cgaactacac
    15181 gccggggtaa aagattttga agagaaacac ggcagtcagc tgttgttgct gattcgtttt
    15241 ttaatgcacg cgagaaattc gggtgttctg aagtggaaag cataaatgac cgagcgcata
    15301 gaatttgttt tgccttaccc gccgacggtg aacacttact ggcgacgccg tggcagcaca
    15361 tattttatat cggaggaggg aaagcgctat cgccgggcag tggcgcttat tgttcgccag
    15421 cagcggctga aattaagcct gtccggacgg ctggcaataa aaattattgc agagccaccg
    15481 gataagcgtc gtcgtgacct ggacaatatt ctgaaagcac cgctggatgc gctgacgcat
    15541 gcgggagtgc ttattgatga cgagcagttt gatgaaatca atattgtacg tggcaagcca
    15601 gtatctggtg gacggctggg ggtgaagatt taccccataa tgcttgaagg gcaggtcaaa
    15661 aaatgaaact ggaagattta ccgaaatact actccccaaa atcccccggc ctgactgatg
    15721 catcggcctc aacgtcgaaa gatgcgctga gtatcactga tgtgatggcc gcgcagggca
    15781 tgacacagaa tcgggctgag atggggtttt ctgcgttcct tgggaaaatg ggcattagta
    15841 tgaatgacag agaacgggca acagaattgc tgacagaata tgcactcagt cggtgtgatt
    15901 gcgtggcggc gttaagaaaa ctcccggcag aaataaaacc ggcagtgatg cgtattatgg
    15961 cttcgtatgc gtttgaagat tatgcccgta gcgcggcgag caaaaaacag tgctcctgtt
    16021 gtcatggaaa aaaatttatt gaaagcgagg tttttacaaa caagatccag tatccggatg
    16081 gtaagccgcc agtgtgggca aagtgcacaa aaggcgtgta tccgtcttac tgggaggaat
    16141 ggaaaaaagt cagggaggtg gtaaaagttg cctgtccgga gtgtggcgga aagggggagg
    16201 tttccaccgc ctgtaaagat tgtcgtgggc gcggtgttgc cattcatcgt gaagagtctg
    16261 aaaaacgggg tatgcctgtt atcagggact gccagcgttg tggtggtcgt ggctatgaaa
    16321 gattgccgtc aacagaggca tttaatgcta tatgcgaggt gacaaaccag ataacacgcg
    16381 cgtcatggga aaaaacagtt aagaaattct atgatgcgct ggtgagcaag tttgatattg
    16441 aggaagcatg ggctgagagg cagttaaaaa aggtaaccag gtaacaaggt tgatttttcc
    16501 ggaatctgtg gtaaatttgc cctaacgatg ggcgttttat gcctgacgtt agaagacttt
    16561 ttacacccgt cgccaggcgg gtttttttat gactgaaatc actccagtac agtaaacgcg
    16621 ctggcggcgg tgaataccgg tctttcagct tgctggcttt ttcgacaaga gttattggtg
    16681 tgtcacgtta accggaaaag ggaaaaagac atgctgaaac agcaggatat gaccgaaacc
    16741 gccagagtgg tgtttaatga attaagcgtc accgaaccgg cgacagtcgg ggagattgcg
    16801 cagaatatgt acctttcacg cgaacgctgt cagttaatac tgacccagct tgttatggcg
    16861 gg
//
LOCUS       DAZKFU020000089        16792 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZKFU020000089 DAZKFU020000000
VERSION     DAZKFU020000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.090"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            100..1239
                     /gene="arnB"
                     /locus_tag="ACHOQQ_RS11845"
                     /old_locus_tag="pgaptmp_002369"
     CDS             100..1239
                     /gene="arnB"
                     /locus_tag="ACHOQQ_RS11845"
                     /old_locus_tag="pgaptmp_002369"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGU4470609.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1243..2211
                     /gene="arnC"
                     /locus_tag="ACHOQQ_RS11850"
                     /old_locus_tag="pgaptmp_002370"
     CDS             1243..2211
                     /gene="arnC"
                     /locus_tag="ACHOQQ_RS11850"
                     /old_locus_tag="pgaptmp_002370"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGU4470610.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2211..4193
                     /gene="arnA"
                     /locus_tag="ACHOQQ_RS11855"
                     /old_locus_tag="pgaptmp_002371"
     CDS             2211..4193
                     /gene="arnA"
                     /locus_tag="ACHOQQ_RS11855"
                     /old_locus_tag="pgaptmp_002371"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGU4470611.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPVCTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4190..5080
                     /gene="arnD"
                     /locus_tag="ACHOQQ_RS11860"
                     /old_locus_tag="pgaptmp_002372"
     CDS             4190..5080
                     /gene="arnD"
                     /locus_tag="ACHOQQ_RS11860"
                     /old_locus_tag="pgaptmp_002372"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGU4470612.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5080..6732
                     /gene="arnT"
                     /locus_tag="ACHOQQ_RS11865"
                     /old_locus_tag="pgaptmp_002373"
     CDS             5080..6732
                     /gene="arnT"
                     /locus_tag="ACHOQQ_RS11865"
                     /old_locus_tag="pgaptmp_002373"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGU4470613.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6729..7064
                     /gene="arnE"
                     /locus_tag="ACHOQQ_RS11870"
                     /old_locus_tag="pgaptmp_002374"
     CDS             6729..7064
                     /gene="arnE"
                     /locus_tag="ACHOQQ_RS11870"
                     /old_locus_tag="pgaptmp_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGU4470614.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7064..7450
                     /gene="arnF"
                     /locus_tag="ACHOQQ_RS11875"
                     /old_locus_tag="pgaptmp_002375"
     CDS             7064..7450
                     /gene="arnF"
                     /locus_tag="ACHOQQ_RS11875"
                     /old_locus_tag="pgaptmp_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGU4470615.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7444..7710)
                     /gene="pmrD"
                     /locus_tag="ACHOQQ_RS11880"
                     /old_locus_tag="pgaptmp_002376"
     CDS             complement(7444..7710)
                     /gene="pmrD"
                     /locus_tag="ACHOQQ_RS11880"
                     /old_locus_tag="pgaptmp_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGU4470616.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLYTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7820..9175)
                     /gene="menE"
                     /locus_tag="ACHOQQ_RS11885"
                     /old_locus_tag="pgaptmp_002377"
     CDS             complement(7820..9175)
                     /gene="menE"
                     /locus_tag="ACHOQQ_RS11885"
                     /old_locus_tag="pgaptmp_002377"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGU4470617.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9172..10134)
                     /gene="menC"
                     /locus_tag="ACHOQQ_RS11890"
                     /old_locus_tag="pgaptmp_002378"
     CDS             complement(9172..10134)
                     /gene="menC"
                     /locus_tag="ACHOQQ_RS11890"
                     /old_locus_tag="pgaptmp_002378"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGU4470618.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRG
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10134..10991)
                     /gene="menB"
                     /locus_tag="ACHOQQ_RS11895"
                     /old_locus_tag="pgaptmp_002379"
     CDS             complement(10134..10991)
                     /gene="menB"
                     /locus_tag="ACHOQQ_RS11895"
                     /old_locus_tag="pgaptmp_002379"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGU4470619.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11006..11764)
                     /gene="menH"
                     /locus_tag="ACHOQQ_RS11900"
                     /old_locus_tag="pgaptmp_002380"
     CDS             complement(11006..11764)
                     /gene="menH"
                     /locus_tag="ACHOQQ_RS11900"
                     /old_locus_tag="pgaptmp_002380"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGU4470620.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQRLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11761..13431)
                     /gene="menD"
                     /locus_tag="ACHOQQ_RS11905"
                     /old_locus_tag="pgaptmp_002381"
     CDS             complement(11761..13431)
                     /gene="menD"
                     /locus_tag="ACHOQQ_RS11905"
                     /old_locus_tag="pgaptmp_002381"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGU4470621.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRVCDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13520..14815)
                     /gene="menF"
                     /locus_tag="ACHOQQ_RS11910"
                     /old_locus_tag="pgaptmp_002382"
     CDS             complement(13520..14815)
                     /gene="menF"
                     /locus_tag="ACHOQQ_RS11910"
                     /old_locus_tag="pgaptmp_002382"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGU4470622.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVFARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14894..15199)
                     /gene="elaB"
                     /locus_tag="ACHOQQ_RS11915"
                     /old_locus_tag="pgaptmp_002383"
     CDS             complement(14894..15199)
                     /gene="elaB"
                     /locus_tag="ACHOQQ_RS11915"
                     /old_locus_tag="pgaptmp_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGU4470623.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15254..15715)
                     /locus_tag="ACHOQQ_RS11920"
                     /old_locus_tag="pgaptmp_002384"
     CDS             complement(15254..15715)
                     /locus_tag="ACHOQQ_RS11920"
                     /old_locus_tag="pgaptmp_002384"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4470624.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15780..16697
                     /gene="rbn"
                     /locus_tag="ACHOQQ_RS11925"
                     /old_locus_tag="pgaptmp_002385"
     CDS             15780..16697
                     /gene="rbn"
                     /locus_tag="ACHOQQ_RS11925"
                     /old_locus_tag="pgaptmp_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGU4470625.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         3861 a         4574 c         4319 g         4038 t
ORIGIN      
        1 tattctatcc ttaaaatttc gctccaaatg gcaaaatata cacaacactc tttatagcaa
       61 atataagtgg acaggtattc aatggcggaa ggaaaagcaa tgtcagaatt tttgcctttt
      121 tcgcgaccag caatgggcgt ggaggaactc gctgcagtta aagaggttct cgaatccggt
      181 tggatcacaa ccggtccgaa gaatcaggcg cttgagcaag ccttttgcca gttgacggga
      241 aatcagcatg ccatcgcggt cagttcagcc accgccggaa tgcatatcac gctaatggcg
      301 ttggaaattg gcaagggcga tgaagtgatt acgccttccc tgacctgggt ttcaaccctc
      361 aatatgattt ccttgttggg tgcaacgccg gtaatggtgg atgtcgatcg cgatacgctg
      421 atggtcacgc ctgaagcgat cgaatcagcc attacgccac gcactaaagc catcattccg
      481 gtgcattatg ccggtgcgcc agcagatatt aacgccattc gcgccattgg cgaacgttac
      541 ggcatcgcag ttatcgaaga tgcagcccat gccgtcggta cgtattacaa agggcgacat
      601 attggcgcaa aaggtaccgc tattttttcg tttcacgcca tcaaaaatat tacctgtgcg
      661 gaaggtggcc tgattgttac tgataatgaa aatcttgccc gccagctacg gatgctgaaa
      721 tttcacggtc tgggtgtcga tgcctatgac agacaaacct ggggccgtgc accgcaggct
      781 gaagtcttaa caccgggcta taagtacaat ctgaccgata ttaacgccgc gattgccctg
      841 acacagttag ctaaattaga gcacctcaac acccgtcggc gcgaaattgc ccagcaatat
      901 cagcaagcac tggcagctct cccctttcag ccattaagcc ttcccgcctg gtcgcacgtt
      961 cacgcctggc atctgtttat tattcgtgtc gatgaacaac gttgtggtat cagtcgaaat
     1021 gcgttgatgg aagcgttaaa agaaagaggt attggtaccg ggttacattt ccgcgccgct
     1081 cacacacaaa aatattatcg cgagcgtttt cccacgctgt cgttaccgaa taccgaatgg
     1141 aatagcgaac gcatctgttc gttgccgctg ttcccggata tgactaccgc cgatgccgac
     1201 cgtgtcatca cagcccttca gcaactcgca ggacaataag ccatgtttga aatccaccct
     1261 gttaagaaag tctcggtggt tattcccgtt tataacgagc aggaaagctt accggaatta
     1321 atcaggcgca ccaccacagc ctgtgaatcg ttggggaaag agtatgagat cctgctgatt
     1381 gatgacggca gtagcgataa ttccgcgcat atactggtcg aagcctcaca agcggagaac
     1441 agccatattg tgtctatttt gcttaaccgc aattacgggc aacattcagc gattatggcg
     1501 ggattcagtc acgttactgg cgacttaatt attacccttg atgccgatct ccagaatccg
     1561 ccagaagaaa tcccccgcct ggtggcaaaa gccgatgaag gttacgacgt ggtagggact
     1621 gtacgccaga accgccagga cagctggttt cgcaaaaccg cttcgaagat gattaaccgg
     1681 cttattcagc gcaccaccgg caaagcgatg ggtgactacg gttgtatgtt gcgcgcctat
     1741 cgccgtcata ttgtcgatgc gatgttgcac tgccatgaac gcagcacctt tatcccgatt
     1801 ctggcgaata tcttcgcccg ccgtgccatt gaaattccag tgcatcatgc cgagcgtgag
     1861 tatggtgaat ccaaatacag ctttatgcgc ctgattaatt tgatgtacga cctggtgacc
     1921 tgccttacca caacgccgct acgtatgctg agtctgctcg gcagcattat tgcgattgga
     1981 ggttttagca ttgcggtgct gctggtgatt ttacgcctga ccttcggacc acaatgggcg
     2041 gcagaaggtg tctttatgct atttgccgtg ctgtttactt ttattggcgc tcagtttatc
     2101 ggcatgggat tgctcggtga atatatcggc aggatctaca ccgatgtccg cgcccgcccc
     2161 cgctattttg ttcagcaagt tatccgtcca tccagcaagg aaaatgaata atgaaaaccg
     2221 tcgtttttgc ctaccacgat atgggatgcc tcggtattga agccctgctg gctgccggtt
     2281 acgaaattag cgccattttt acccataccg ataatcccgg tgaaaaagcc ttttatggtt
     2341 cggtggctcg tctggcggcg gaaagaggca ttccggttta tgcgccggat aacgttaatc
     2401 atccgctgtg ggtggaacgc attgcccaac tctcaccaga ggtgattttc tctttttatt
     2461 atcgccatct tatttgcgac gagattttgc agctcgctcc cgcaggtgca tttaatctgc
     2521 acggttcgct gttaccaaaa tatcgtggtc gcgcgccgct gaactgggtg ctggtgaacg
     2581 gtgaaacaga aactggcgtt acattgcacc gaatggtaaa acgtgccgat gccggggcca
     2641 ttgtggcgca actgcgcgtt gccattgcgc cagacgatat cgctattacg ctgcatcata
     2701 aattgtgcca tgccgcgcgc cagctactgg aacagacatt acccgccatt aaacacggta
     2761 atattctgga aatcgcccag cgcgaaaacg aagccacctg ttttggtcgc agaacgccgg
     2821 atgacagttt cctcgaatgg cataagccgg catccgtact gcacaacatg gtacgtgccg
     2881 ttgccgatcc gtggccgggt gccttcagct atgttggcaa tcagaaattc accgtctggt
     2941 cgtcgcgtgt tcatcctcat gccagcaaag cacagccggg gagcgtgatt tctgttgcgc
     3001 cactgctgat tgcctgtggc gatggcgcgc tggaaatcgt caccggacag gcgggcgacg
     3061 gcattactat gcagggctcg caattagcgc agacgctggg cctggtgcaa ggttcacgct
     3121 tgaatagcca gcctgtctgc accgcccgac gccgtacccg ggtactcatc ctcggggtga
     3181 atggctttat tggcaaccat ctgacagaac gcctgctgcg cgaagatcat tatgaagttt
     3241 acggtctgga tattggcagc gatgcgataa gccgttttct gaatcatccg cattttcact
     3301 ttgtcgaagg cgatatcagt attcattccg aatggattga gtatcatgtc aaaaaatgtg
     3361 atgtcgtctt gccgctggtg gcgatagcca cgccgattga atatacccgc aacccgctgc
     3421 gcgtatttga actcgatttt gaagagaatc tgcgcattat ccgctactgc gtgaagtacc
     3481 gtaagcgaat catcttcccg tcaacttcag aagtttatgg gatgtgtagc gataaatact
     3541 tcgatgagga ccattctaat ttaatcgtcg gcccggtgaa taaaccacgc tggatttatt
     3601 cggtatcaaa acaattactt gatcgggtga tctgggccta tggcgaaaaa gagggtttac
     3661 agttcaccct cttccgcccg tttaactgga tgggaccgcg gctggataac cttaatgcag
     3721 cgcgaattgg cagctcccgc gcgattacgc aactcattct caatctggta gaaggttcac
     3781 cgattaagct aattgatggc ggaaaacaaa aacgctgctt tactgatatt cgcgatggta
     3841 tcgaggcgtt ataccgcatt atcgaaaatg cgggaaatcg ctgcgacggc gaaattatca
     3901 acattggcaa tcctgagaac gaagcgagca ttgaggaact gggcgagatg ctgctggcga
     3961 gcttcgaaaa acatccgctg cgccatcatt tcccaccgtt tgcgggcttt cgcgttgtcg
     4021 aaagtagcag ctactacggc aaaggatatc aggacgtaga gcatcgtaaa ccgagcatcc
     4081 gcaatgccca ccgctgcctg gactgggagc cgaaaattga tatgcaggaa accatcgacg
     4141 aaacgctgga tttcttcctg cgcaccgttg atcttacgga taaaccatca tgaccaaagt
     4201 aggcttacgc attgatgtcg atacctttcg tggcacccgt gaaggcgtgc cgcgtctgct
     4261 ggaaatcttg agtaagcata atattcaggc cagcattttt ttcagcgtcg gcccggacaa
     4321 tatgggccgt catctctggc gactggtgaa gccacagttt ttgtggaaga tgctgcgctc
     4381 aaacgcggca tcgctttatg gctgggatat tttactggca ggtacggcct ggccaggtaa
     4441 agagattggt catgccaatg ccgatatcat tcgtgaagcg gctaaacatc acgaagtcgg
     4501 cctgcacgcc tgggatcacc atgcctggca agcccgtagc ggtaactggg atcggcaaac
     4561 aatgatcgac gatattgcac gcggccttcg cactctggaa gagattatcg gtcaaccggt
     4621 aacctgttct gccgctgcgg gctggcgtgc cgaccagcag gtgatcgaag caaaagaagc
     4681 gttccatttg cgctacaaca gcgattgtcg tggggccatg ccgttccgtc cactgctcga
     4741 atcaggaaac cctggcactg cgcaaattcc ggtgacctta cccacctggg atgaagtgat
     4801 tggtcgggat gtgaaagcag aagattttaa cggttggtta ctcaaccgca tcctgcgaga
     4861 taaaggcacg ccggtttata ccattcatgc agaagttgaa ggctgcgctt atcagcataa
     4921 ttttgtggat ctcctcaaac gcgcagctca ggaaggcgtg acattttgcc ctttaagcga
     4981 actgttatca gagacgttgc cgctcggaca agttgttcgc ggaaatattg ccggacgtga
     5041 aggctggctg ggttgccaac aaattgcggg tagtcgctga tgaaatcggt acgttacctt
     5101 atcggcctct tcgcgtttat tgcctgctat tacctgttac cgatcagcac gcgtctgcta
     5161 tggcaacccg atgaaacgcg ttatgcggaa atcagtcgag aaatgctggc atccggcgac
     5221 tggattgtgc cccatctgtt agggctacgt tatttcgaaa aacccattgc cggatactgg
     5281 attaacagca ttgggcaatg gctatttggc gcgaataact ttggtgtgcg ggcaggcgtt
     5341 atctttgcga ccctgttaac tgccgcgctg gtgacctggt ttactctgcg cttatggcgc
     5401 gataaacgtc tggctctact cgccacagta atttatctct cattgtttat tgtctatgcc
     5461 atcggcactt atgccgtgct cgatccgttt attgccttct ggctggtggc gggaatgtgc
     5521 agcttctggc tggcaatgca ggcacagacg tggaaaggca aaagcgcagg atttttactg
     5581 ctgggaatca cctgcggcat gggggtgatg accaaaggtt ttctcgccct tgccgtgccg
     5641 gtattaagcg tgctgccatg ggtagcgacg caaaaacgct ggaaagatct ctttatttac
     5701 ggctggctgg cggttatcag ttgcgtactg acggttctcc cctggggact ggcgatagcg
     5761 cagcgggagc ctgacttctg gcactatttt ttctgggttg agcatattca acgctttgca
     5821 ctggatgatg cccaacatag agctccgttc tggtactacg tgccggtcat cattgccggt
     5881 agcctgccgt ggctgggatt actccccggt gcactataca caggctggaa aaaccgcaag
     5941 cattccgcaa ccgtctattt gttgagctgg acgataatgc cgctgctgtt tttctccgtt
     6001 gctaagggta aattgcccac ctatattctt tcttgctttg cacctttggc aatgctgatg
     6061 gcgcattacg ctttgctggc agcaaaaaat aatcctctgg cgctgcggat taatggctgg
     6121 attaacatcg cttttggcgt cactggcatt attgccacat ttgtggtctc cccctgggga
     6181 ccaatgaaca cgccggtgtg gcaaaccttc gagagctata aagtcttttg tgcctggtcg
     6241 attttttcgc tatgggcatt tttcggctgg tacaccttaa caaacgtcga aaagacttgg
     6301 tcttttgccg cgctttgccc gctggggctg gcgttgctgg taggattttc aattcctgac
     6361 agagtcatgg aaggaaaaca tccgcaattt tttgtcgaga tgacacaaga atcacttcag
     6421 ccaagccgct atattcttac tgatagcgtc ggtgttgccg caggtctggc atggagcctg
     6481 caacgcgatg acatcatcat gtatcgccag acaggtgagt tgaaatacgg ccttaattat
     6541 ccggatgcga aagggagatt tgtcagcggt gatgagttcg caaactggct taatcaacat
     6601 cgtcaggagg ggattattac actcgtgctt tcggttgacc gcgatgaaga tatcaacagt
     6661 ctcgccattc cgcccgcaga tgccatcgat cgtcaggagc gtctggtgct gattcagtat
     6721 cgtcccaaat gatctggcta acattagtct ttgccagctt gcttagcgtt gccgggcagt
     6781 tgtgtcagaa acaggcaacc tgctttgtgg cgataaacaa acggcgcaaa catatcgtgc
     6841 tgtggctggg actggcgctg gcttgtcttg gtcttgccat ggtgctctgg ctgctggtct
     6901 tgcagaacgt accggtaggc attgcctacc cgatgttaag tctgaatttt gtctgggtga
     6961 cgctggctgc agtaaaactg tggcacgaac cggtatcgcc gcgtcactgg tgtggggtgg
     7021 cgttcattat tggtggcatt gtgatcctcg ggagtacggt gtaatgggcc tgatgtgggg
     7081 attattcagc gtcattattg cctcagttgc gcaattaagc ctgggttttg cggcgagtca
     7141 tctgccgccg atgacgcact tatgggattt tatcgcggct ctgttagctt ttgggctgga
     7201 tgcccggata ctgttactcg ggctgctggg ttatctgctt tccgtttttt gttggtataa
     7261 aacgctacat aaacttgcgc taagcaaagc ctatgccttg ctcagtatga gttatgtgct
     7321 ggtatggatt gcttcaatgg tacttcccgg ctgggaaggg actttttcgc tgaaagcact
     7381 actgggagta gcctgtatta tgagcgggtt gatgctgatt ttcctgccca cgacaaaaca
     7441 acgttactga gttttccctg ccactttaca ctgccgttcc cactcatcgg ggctataaca
     7501 gcttgcagag aggactttca ccgtatacag cttttcacga ttaatacaat aaagcgcatt
     7561 ctgcaaaggc gagagtaaat cccccacttt cacggcgaag tcactcttca cttcggcgat
     7621 catttttatc gcgccgccag catcgcagag cataagtaca tgtctgttat cttgtttatt
     7681 gcagcacgat tttttgacca gccattccat tgtattatcc tgtttgctaa gagttttcat
     7741 atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg cagaactgag
     7801 aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc gcctgacgtg
     7861 aaattttaat accgccgttt ttcagctccg gcggcaaagt cagccaacgt accggttgct
     7921 gaaaacgggc tagcttatct ttcacccatt cactaagatc aacgctctcg tggtcatact
     7981 ccatcaccgc caccggtcga tgaccaaact ccttgtcggc aacggggacg ataaacacct
     8041 gcaaaaccgc aggatgtgca gcaattacgc gctcgacttc ttccggctga ataccctctc
     8101 cgccactgaa gaatagattg tctaaacgtc cgacaatggt cagcttgcca ttatgcatct
     8161 caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc agttgcccgt
     8221 tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta ttaacgattt
     8281 tcacttcccg acccggcagc ggcgaaccaa cgtctgccag gccgtcggct tctttcgcac
     8341 acaccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc ccctgctcgc
     8401 gcgcctgttc cgtcaactcg accgggatag ccgcaccgcc aagtaacacc gctttcaggg
     8461 aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt gaagcgtgag
     8521 tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc cgcgcaccag
     8581 cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt aaagagagca
     8641 accagtcatc gtgatcgcca aacggaatca acgataacac cccttcggca ctggcaagat
     8701 gggcctgata agtatggaca gcggctttcg gcaacccggt agaacccgag gtcaacgtca
     8761 ttgagcacag acgcgtcggc tgccacgcag cggcatgtgc gccttcaacc agctgaatgt
     8821 gcagcgacgt taacgccgga aacgtgtttt ccccatccgg caccagcgca aattgcagcg
     8881 tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc acgggcaaca
     8941 cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc gtattccacg
     9001 cccgcaacat cacgccgctg ccctcaacca caccctgcac cgcaaatccg gaggctaatt
     9061 catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga cgtaaggcga
     9121 tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag atcataacaa
     9181 ccgctccagt gcatcaactt ccacgacagg cagcgtgcta cccggccagc gacgtacctg
     9241 ctgcgcctgc atcagatcca gcgtgtccag ccctggaatg gtgtccggcg ttaaccaggc
     9301 ggcaatccgc gccagttgcg ttaagcctaa gctcgattca atggaagaac tgatcaccgc
     9361 cgtcagcccc agcgcgtgcg ccgcctgtac ctgctcgcgt actttttcca gactgcccgt
     9421 gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg caaaatccgg
     9481 cccgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa acgctcgcga
     9541 atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtcgcgat aatccgggtt
     9601 aacgtatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg cgtcaagacg
     9661 caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt cgcgcaccgc
     9721 ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg caagtttgag
     9781 gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg ctgcttgcgg
     9841 caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg aaggcatctg
     9901 cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca cactttgcgc
     9961 ctcttcccag gtttcctgac tgaagcccgg cagtggggag atctcccccc acccttcgcg
    10021 ctcgccttca cgcaggcaaa catacagccc gtcgcgggtt tttaaccgcc tgtcgcgcag
    10081 aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac gcattacgga
    10141 ttccgtttga atttgctgaa gtcaggctga cgtttctggt tgaaggcgtt gcgaccttcc
    10201 tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc ctgcagcccc
    10261 gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc catcgggctg
    10321 ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc cagcggtacc
    10381 acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg gcacaggaac
    10441 cagatttcac gcgctttttt ctgcccgacg atacgagcca tgtaggaagc gccccagccg
    10501 ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc tgccgcgata
    10561 gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc agccaccatc
    10621 gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac gttcaggtga
    10681 tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg gtcaccaccg
    10741 gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgacgtt atcgtcataa
    10801 cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa ggcattgcgc
    10861 acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc ataacgaatg
    10921 tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaagcat tgcttcatca
    10981 ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga cttgcgatta
    11041 cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca tggcagtcgg
    11101 cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat aaataataaa
    11161 acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg agagaagtcg
    11221 cctccagcat ggcggcaagc gttgcgccat tattgttgct gcgcagcgcc accagctccc
    11281 ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg gcaaataccg
    11341 cggttaatgg ttcggaacga aaacgctgcg cccattggcg atcggaacgc tgacgttccg
    11401 cgcgttgttc agcattttgc agccccggat gaccgccttc gacaacaacc ccacaaagcc
    11461 ccgccagcct ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag taccccacca
    11521 gccagaagtt aaggatgttg taactaacca gggttttacg cagtaagtcg gtgacatcat
    11581 caaatccatc gacgctaatc gccgccgaac caccgtgacc tgggagatca acatacaacc
    11641 gtgagtagtc ggcaaacacc tcgcccactt cttgccattc gtggcaatcg ccggaaaaac
    11701 cgtgcaaaaa caccagccag ggtaaacctg gtttgccgtg ttttgcctgc gcgtgcagga
    11761 tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcgccgt cggtgtcgtt
    11821 aaccaccatt tcaattaccg tggtggttgg cgtgcgccag gcatcggcaa gtgccgtttc
    11881 aagttcctgc cagttttgcg gacgatgata tttcagctcg aacatcgcgg cggcgtgctc
    11941 aaaatggacg ttttgcggca tcagatagaa acgctcgcgc tcgcttttcg gcgttggcag
    12001 cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg gcgcggaaac
    12061 ctggcgcaat aacgccagcg cattcaggtc gtaaagtgcg gagagatcgc ccacaatcgc
    12121 cagcgtcggt ttgccgcttg cccgctgaac gccggcggca gtcgagagca gcccgtcgat
    12181 accgctggcc ccacggttgc tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc
    12241 aatcagacgt accaccaggc tgttaccgac aaacaattgc ccctgttcag gcagatagtc
    12301 gcagacgcga tgcgccagtt gcgcttcgcc aaacgcatcg cgacgggcaa taaccgcctg
    12361 catcgcctgc tccgccaggc gcgggatttc aacgcaccag ggctggcgtt tttctgccgg
    12421 atgcagttcc agccagtcgg caatattggc aattaaccga cgcccgcgat ggtgcgccgg
    12481 atcaagccgc ccttcgatgt catccacaat ccagtactct tcgggttcac agcttgcctg
    12541 ccattgcagg agccgtttgc cagtcaggct gcttcccagt tgcaccacaa tttgcgcctg
    12601 ttgcagttcg ctggtcgctt tggcattgcc taaccagaga tcggcacacg gcagcggctg
    12661 tccggtttgc gacagcacgt cgccaatcag cggccagcca agagtttgcg cccacagtgc
    12721 aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc gtttttgccg
    12781 ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac gcagccacgg
    12841 cttatcgtcc tgccaccagt cgcccagacg ctgttgccag ctaagcccgg tatcgtccat
    12901 ttcgccatac agcggttcag caaacgggca gttgatatgg actccacccg catgaagcgt
    12961 accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct gggtcgggcg
    13021 cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc gaattgcctg
    13081 attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa tcagtttttc
    13141 tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg taccggaggt
    13201 gacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca gatgccccag
    13261 tccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg ccgctaacgt
    13321 taacggtgta gaacgcgagc caggggcgat acagatgtgt cttacgccgt gacgcgttaa
    13381 tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc
    13441 cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg atatgaatcg
    13501 gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct gccgctttgt
    13561 tgtcgatttc ctgccactct tgctcggggt cggagccacg gacaatgccc gcgccagcat
    13621 atagccgcac gacattgccg ctgatttttg ctgagcgcag ggaaacacag aattcgcttt
    13681 gctgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac ggttcgtgac
    13741 gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc gttggctgca
    13801 actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata cagcggcgaa
    13861 gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc gtctgggtat
    13921 cggcctgtaa tcgttggcag atgtcttcca ccaccagcat gttctcgcgc tggtttttat
    13981 catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga tgatttgcta
    14041 ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat aaccgttccg
    14101 gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg tagcaattga
    14161 gattcagtcg acgactggcc gccatcatcg ccgccgcatt gaccggactt gcgaaatgca
    14221 ggtcagttgc ccgagcaaac accactttgt cgagctcacc ttcggcgatg gttttcgttg
    14281 ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct cgcgtagtgg
    14341 ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat tctcttgcct
    14401 taatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc gtggctttac
    14461 cgccacagcg tcgccattcc aggcgtggta aaagtaaatt gccctgcgac gggtcaaacg
    14521 cattcagccc ccaaatgcgt aagtcggcgt gttccgggtg ctggcgaaga aaacgttgtg
    14581 cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct tcatcaccat
    14641 tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag ctcaaggcat
    14701 caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt gtcgccggaa
    14761 tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat tgcacgcgga
    14821 cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg cagtaccccg
    14881 gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagccca acggccgcac
    14941 ccacaccaat tccctgccag ggtttttcgt ggacgtagtc atcagcacga taaacagcct
    15001 gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc acatcatcca
    15061 gcgctttttc tgcacgcgct ttcagctcaa catatttctg atcggcggga tcgccagagg
    15121 agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg atacgtgtat
    15181 caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac taactataga
    15241 caagggttat tgattacgcc tgaattacct cgcgtgccat cccaatgtgt gggataccat
    15301 cttcttcata gatctccgtc acagggataa aaccgaaact ctggtagaag ttttgcagat
    15361 gcgcctgcgc cccgaggtag acaggcttat caggccagtg atgcgtgcaa gtgtcgaggg
    15421 ttttactcat cagttgctgc cccacttttt cgccacgcaa cgcttcactg acaatcaccc
    15481 gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca tacgccacca
    15541 gttcatcatt tttccaaccg agaatatggc gattatcccc tgtcaaatca tcaccatcga
    15601 tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt tgtaataagg
    15661 cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca atcataatgc
    15721 tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt gatgaactca
    15781 tggaattaat ttttttaggt acttcggccg gtgtgccaac ccgtacgcgc aatgtcacgg
    15841 caatattgct taacctgcaa catccgaccc agagcggtct ttggttgttt gactgcggtg
    15901 aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg gacaagattt
    15961 ttatcagtca ccttcatggc gatcatctct ttggtttacc cggcttgctg tgcagtcgtt
    16021 ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc cgtgaatttg
    16081 tggagaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa attgtcgaaa
    16141 ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt gaccgcttat ccaatggaac
    16201 acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt gcattaaatg
    16261 cccaggcatt aaaagctgct ggcgtaccgc ctggcccact atttcaggaa ttaaaagcgg
    16321 gcaaaacaat catgctggaa gatggaaggc agattaacgg cgcagattac ttagccgctc
    16381 cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat gccgcacttg
    16441 acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata accatggaag
    16501 ccaaagccaa tagtcgcggt catagctcta cacgccaggc tgcgacactg gcccgtgagg
    16561 ctggagtcgg taagctaatc attacccacg tcagctcgcg ctatgatgat aaaggttgtc
    16621 agcacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg aatgatttca
    16681 ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gcgaaacttg ccctgttatc
    16741 gcaacccgcg ctacatatat ccacccacac gggcatattg agcgattttt ga
//
LOCUS       DAZKFU020000090        16706 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZKFU020000090 DAZKFU020000000
VERSION     DAZKFU020000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.091"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(86..622)
                     /gene="yghD"
                     /locus_tag="ACHOQQ_RS11930"
                     /old_locus_tag="pgaptmp_002386"
     CDS             complement(86..622)
                     /gene="yghD"
                     /locus_tag="ACHOQQ_RS11930"
                     /old_locus_tag="pgaptmp_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HGU4470626.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIIAARKSATTAPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLNALDEKYVLRVTQIDVS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(624..1802)
                     /gene="gspL"
                     /locus_tag="ACHOQQ_RS11935"
                     /old_locus_tag="pgaptmp_002387"
     CDS             complement(624..1802)
                     /gene="gspL"
                     /locus_tag="ACHOQQ_RS11935"
                     /old_locus_tag="pgaptmp_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HGU4470627.1"
                     /translation="MSSILEIFFPLCASDTIRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGYMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGSWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRDQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1799..2776)
                     /gene="gspK"
                     /locus_tag="ACHOQQ_RS11940"
                     /old_locus_tag="pgaptmp_002388"
     CDS             complement(1799..2776)
                     /gene="gspK"
                     /locus_tag="ACHOQQ_RS11940"
                     /old_locus_tag="pgaptmp_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HGU4470628.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLALQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2773..3378)
                     /gene="gspJ"
                     /locus_tag="ACHOQQ_RS11945"
                     /old_locus_tag="pgaptmp_002389"
     CDS             complement(2773..3378)
                     /gene="gspJ"
                     /locus_tag="ACHOQQ_RS11945"
                     /old_locus_tag="pgaptmp_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HGU4470629.1"
                     /translation="MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVTGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQENWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(3375..3746)
                     /gene="gspI"
                     /locus_tag="ACHOQQ_RS11950"
                     /old_locus_tag="pgaptmp_002390"
     CDS             complement(3375..3746)
                     /gene="gspI"
                     /locus_tag="ACHOQQ_RS11950"
                     /old_locus_tag="pgaptmp_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HGU4470630.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPQNTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(3743..4306)
                     /gene="gspH"
                     /locus_tag="ACHOQQ_RS11955"
                     /old_locus_tag="pgaptmp_002391"
     CDS             complement(3743..4306)
                     /gene="gspH"
                     /locus_tag="ACHOQQ_RS11955"
                     /old_locus_tag="pgaptmp_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HGU4470631.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLSQCDERMP"
     gene            complement(4310..4765)
                     /gene="gspG"
                     /locus_tag="ACHOQQ_RS11960"
                     /old_locus_tag="pgaptmp_002392"
     CDS             complement(4310..4765)
                     /gene="gspG"
                     /locus_tag="ACHOQQ_RS11960"
                     /old_locus_tag="pgaptmp_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HGU4470632.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4782..6005)
                     /gene="gspF"
                     /locus_tag="ACHOQQ_RS11965"
                     /old_locus_tag="pgaptmp_002393"
     CDS             complement(4782..6005)
                     /gene="gspF"
                     /locus_tag="ACHOQQ_RS11965"
                     /old_locus_tag="pgaptmp_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HGU4470633.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPMHIEA
                     RMNASAGGMLQRRRHAHRRVASADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPATTRALIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPVMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6005..7498)
                     /gene="gspE"
                     /locus_tag="ACHOQQ_RS11970"
                     /old_locus_tag="pgaptmp_002394"
     CDS             complement(6005..7498)
                     /gene="gspE"
                     /locus_tag="ACHOQQ_RS11970"
                     /old_locus_tag="pgaptmp_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HGU4470634.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDAHTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7498..9558)
                     /gene="gspD"
                     /locus_tag="ACHOQQ_RS11975"
                     /old_locus_tag="pgaptmp_002395"
     CDS             complement(7498..9558)
                     /gene="gspD"
                     /locus_tag="ACHOQQ_RS11975"
                     /old_locus_tag="pgaptmp_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HGU4470635.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9588..10547)
                     /gene="gspC"
                     /locus_tag="ACHOQQ_RS11980"
                     /old_locus_tag="pgaptmp_002396"
     CDS             complement(9588..10547)
                     /gene="gspC"
                     /locus_tag="ACHOQQ_RS11980"
                     /old_locus_tag="pgaptmp_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HGU4470636.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIARGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTVVSPSVNKPPRTDAKTFDKNDVQLISQQNWF
                     GKYQPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLVEEERSTVAVTNKKAISDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(10565..10975)
                     /gene="gspS2"
                     /locus_tag="ACHOQQ_RS11985"
                     /old_locus_tag="pgaptmp_002397"
     CDS             complement(10565..10975)
                     /gene="gspS2"
                     /locus_tag="ACHOQQ_RS11985"
                     /old_locus_tag="pgaptmp_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HGU4470637.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCAGHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQITQTPDAFLTSYQRQMCADPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11041..11850)
                     /gene="pppA"
                     /locus_tag="ACHOQQ_RS11990"
                     /old_locus_tag="pgaptmp_002398"
     CDS             complement(11041..11850)
                     /gene="pppA"
                     /locus_tag="ACHOQQ_RS11990"
                     /old_locus_tag="pgaptmp_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HGU4470638.1"
                     /translation="MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12177..>16706)
                     /gene="sslE"
                     /locus_tag="ACHOQQ_RS11995"
                     /old_locus_tag="pgaptmp_002399"
     CDS             complement(12177..>16706)
                     /gene="sslE"
                     /locus_tag="ACHOQQ_RS11995"
                     /old_locus_tag="pgaptmp_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HGU4470639.1"
                     /translation="LAAILSATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPN
                     PEPTPEPTPDPEPTPEPIPDPEPTPEPEPEPVPTKTGYLTLGGSQRVTGATCNGESSD
                     GFTFKPGEDVTCVAGNTTIATFNTQSEAARSLRAVEKVSFSLEDAQELAGSDDKKSNA
                     VSLVTSSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAAT
                     DKAPSTHTSPVVPVTTPGTKLDLNASFVSANAEQFYQYQPSEIILSEGRLVDSQGYGV
                     AGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRG
                     ANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLGEGEQVVN
                     LPNEFIEQFNTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDE
                     DYKSVTKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAW
                     DKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPHYNSILRCPN
                     GYSWNGGVNKDGQCTLSGDSDDMKHFMQNVLRYLSDDKWTPDAKASMTVGTNLDTVYF
                     KRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGNDPYA
                     IPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLS
                     MALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTIDDTTKEVIWKYQQE
                     NKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKKRILDAFPGLEECK
                     DSDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADLGTNIQRLYQ
                     HELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGFKTFTEFLNC
                     YTNDAYTDGTQCSADLKKSLVDNNMIYGESSGKAGMMNPSYPLNYMEKPLTRLMLGRS
                     WWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTIK
                     SNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYIK
                     GNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESASFVYTTPKKNLNASN
                     YTGGLEQFANDLDTFASSMNDFYGRDETSGKHRMFTYKALTGHKHRFTNDVQISIGDA
                     HSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYM
                     QDRYLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQLKEWAEKNFDIKKW
                     YPDGTPLPEFYSEREGMKGWNLFQLMHRKARGDDVGNDKFGNRNYCAESNGNAADKLM
                     LCASWVAQTDLSEFFKKWNPGANAYQLPGVSEMNFEGGVSQSAYETLAALGLPKPQQG
                     PETINQVTEHKMSAE"
BASE COUNT         3650 a         4778 c         4258 g         4020 t
ORIGIN      
        1 gaaaaaaatt agcttataaa gtctggggga attactctcg ccacgttaac gagagtaatt
       61 ttattgatat taatctcctg aaactttacc ctcgtccaaa ctccagccgc tgcacattca
      121 ccatcccagt cttctcagca gcactgacat caatttgtgt tacccgcagc acatattttt
      181 catccagtgc gtttaaccat ttcagaaggt cattaaacac cacaggttct atccagacct
      241 gaatattctc cccacgctcg gctatccgct tgatgaccac cgagtgcgcg gaagcactgt
      301 cactgatgac ccgcgatacc tgcgcaggtg ctgtcgtggc ggattttcgc gctgcaataa
      361 tatccggcgc ggcgctcctc agtcgggtgt tcatcgccac cagttgctgc aacatcgtct
      421 cctgttgttc tatccgttcg ctcaacggct gccagatgag gacgtaatat ccgacgctaa
      481 acaggaacac caccgctgcc agtaacatgc ccttttcacg cggagaacgc cccgccaggt
      541 gttgtgccag ccagtgttcg ccacggctta actggcgttc acgccattgc tgaaaatagt
      601 gaataaattt atcgcgtaac atgttatttc ctccgcaacg ttacgccgcc ggaaaccgca
      661 tcaccctctt tctgtaacgc gtcctgttgc acaacataat cggttgccag tgcactacgc
      721 agtttgtcga aactggcaaa gtttgctgcc cgcaactgga ggtgaagcgt ctggcgtttt
      781 tgatcaaagg tgaaaccacg catctcgatg tcggaaagtg acgctgattt cagggtgctg
      841 gctatcgctg acaactcggc gagcagccgg gtatcgtcgg cctgtgggcg atattttttc
      901 agcgccatcg tcacctgaga gcgtaaattc acaatccgct tctgctccgg gaacaacgtt
      961 aagaactgtt tctccgcctg gtcgcggctt tgcgccacct gttcgctgac gctccataac
     1021 gtcacgcccc gttccactgc cagcgcaacc agaatcagca atatcggcag aatcatcacc
     1081 cgccagcgcg cccactgttt tcggtagctg acgcgaggct gccacgaccc tgttagcagg
     1141 ttcccttccg gttcgccata agtggtaatg gcgggcagag ccgtaacggt caggcgttca
     1201 gcatctgcgc cagcccatgc tgatagctct tccggtgcaa tgccgactac ggttagcgaa
     1261 agcggtaaat cctgctcatt tagctgggcg cggaacatga ccggagccag cgcccgcccg
     1321 gcgctccatc cccggcattc atcgatgcgg cagataaccc gttgcgcatc gccagccata
     1381 tagccacaag gtatggacat ccagtctggc gcgacggtgg cgcgggtgat gccgtttgcc
     1441 tgcaaccact gcgcaatgtt gcgcatatgc tgttggtgaa tcactgccac ggttgccagt
     1501 tgctggtcga ttttcaacgg ggcgaaatgc agttcatcga tatcctggtt cagctcttct
     1561 tccagtaagg cgggcagaat agtcggtatc tgcttgcgtg ggacatcagg cagttcaacc
     1621 tgccagacgc tgatccattc gccgggaatg tagagtcgaa tcgcatcagt ttgcagccat
     1681 tgctggagac gttcgtcagc aacgtcaggc cagataccgt gttccacgtc gggtgtacgg
     1741 cgctgccagc ggatggtgtc ggaggcgcaa agcgggaaaa aaatctcaag gatggaactc
     1801 actcactttc tcctgtctga tgccagagaa cagaaaagtg ttgtgggccc atgcggacaa
     1861 ttaacgagtt catcgtcagt tcaatctcat tcacggtgat atctgaacgc agccagaagt
     1921 aattgctgtc cacgctcagg acggttttta gctgtttttt agtacgctca tcgacgtcag
     1981 caagtagcgg ctgtgcaaga aactgatcga catcttccca gcccttcgcc ggacgttgtt
     2041 gtaataacgc ccgcgcctga acagggctta accacgggtc aaacagcgcc tcaagaatca
     2101 cactttgcgt gacgtctaaa gtattgatgt tgatttgctg gcgggtcatc ggcagcgcac
     2161 agaccagcgg tttcagtttt tgataaagcc cggcgtccat tccctgcacc acgcgcatct
     2221 cgctgatatc ggccagcggt tgattggcgg cgtagaaagg caccgagcgg gcgagatatt
     2281 cgctgtcttc acggcccaga cgcgtctgca cgctgcggtc ttcatcaata aactcccaca
     2341 ggctttcggc tatcagttcg gcccgataag caggcacatc gaggcgcgag atcagggcaa
     2401 tcagttgttg taacgcgagc ggacgcgacg ccgtcgtcgg ctgagcgagg gcattcaggt
     2461 taaagcacgc ctgtgcgtca cgcagagtga cggcgatttg cccctgcggc agtgggaaaa
     2521 aacgagggcc ggaagcccac ggctgtgcca gatgcacgcg cttttcattt ttcaggctca
     2581 gactgagtgc actcagcgcc aggctttctg cactggcgct gtaccacagc gcctgctggt
     2641 actcctgctg gctgcgggtt cgcccaagtt gttgctgcat ccgcccggaa agcgtgatgg
     2701 ttaccagcat cataaccgcc agcaatacca gcaccacaac cagtgccatt ccgcgttttg
     2761 gtggtgaggt gatcatgata attgcggccc gcgtaacaac cagatgcgtt caatctcgcc
     2821 ccattgcggc gaatgcaggg tcatgcgcac cgccacaggg atcgcctgta ctgacgacca
     2881 gttctcctgc cagcgcgtac catcatagaa ctgcaagcgg agcgaatccg ccggaatcaa
     2941 cttttgcatt gtcggcttca cgctgcctgc tgcgtcggtc agcggccagg ctaaccgttc
     3001 gagatagccg tcatgaatgc ggtaaccgac ggtgagcaga ttgctgcgtg gcagacgcat
     3061 caacggatta accacgccgc cacgcacaaa gcgcatccct tcactctcag atgccagcac
     3121 gccagcgcca gccagtaacg ctggttctcg ctggccctga tcgcctctta ccggacgcgg
     3181 catcatttgg gtcagatcgt gggtcagaaa actcatcgtt tgctgcatga ggtttagttt
     3241 ttgatcgtgt ccggtgacgg cgctattcac gcgcgtgacg ccgttagtca cctgctgcgc
     3301 catcagtgcc agcgaggcaa aaatggcaat tgccaccagc atttccagta acgtgaaacc
     3361 agcgcgagcc cttctcactg ttggcctccc acggcgctaa accaggcgcg tcgtgactga
     3421 attaccgacg aaaagtcttc gtgaaggctg acttcaatat ccaccgcctg gagcagcgcg
     3481 ttgcctgtat tctgcggtgt ggttcgccag taccagcggc ttccagccat atcgctctcg
     3541 ccctgatgcc tgaccgcccg ttgttcacgg gtcatcaggt agagcagcgc ggtctggttt
     3601 tcagccaccc agcctgctac cgttttctcc tcaagaacgt gctgattact cagcgcaccg
     3661 ctggcaatct gtaacaccgc cgtggcagcc agcgcaaaaa tcgccagtgc gagcatcact
     3721 tccagcaagg taaatccgcg cttcatggca tcctctcatc acattgactg agggataacg
     3781 cgccatcgtg tgccagtttt accgcccaac atgcgttttg cgccgctgag tagaagcgca
     3841 gcgtaaacgg cgtggcgggt tcaaaaggcg aaaaacggat ctgcggcgtc ttctttttcg
     3901 cctctttttg caactccagt tcaatatcgt gcagcgtcag gcgacgacgt tgcagctcca
     3961 gcgcatattc cttctgccag atatcacttc cgggttgtaa cagcatctgc acctgtttcg
     4021 gcaccgtaac ctgtgccgat aaccgggtcg cagaaacggg caaccactgc ccctgacgac
     4081 gttgcataaa ctggtatccc ggcgggtcga ttaacacacc aagtgtttgc ccttcgatca
     4141 ctgccctgtc cttaaactgc gcaaagcgag tcagaaaatc ctgcgccgct tttttcgcgg
     4201 gcgattcaga atcggtcgca aaagtctgta ccacgcccgc actggcaagg ccgataagga
     4261 aaatcaccag catgatttcc agaagtgtga atccgcgttc aggcactgct tactgaaact
     4321 cctgcaaatt ccagttaccg atatctgcgc cagcgccttc cccgttttcc tgaccatccg
     4381 cccccagggt ataaacatca aacagccctt tttcacccgg gctgagatac tgataatcat
     4441 tgccccacgg atcctttggc agtcgtttaa tgtatccacc agtacggtag ttgcgggcgt
     4501 ccgccatatt ggctggctgc tggatcagcg cctcaagccc ctgctcggta gttggataac
     4561 gcccgttatc cagtcggtac atatccagtg cgttttccag cgccacgata tcgctgatgg
     4621 ctttttgccg atccgctttc tctttattgc ccaacaggtt aggcaccacc agacttgcca
     4681 ggacgccaag aataacaatc accaccatca cttccagcag ggtaaaacct gcccgtggtt
     4741 tttgtgtgcg ggataacgaa ttcatgtgat aactccgtaa attacattcc aaccatattg
     4801 ttcagttgca gcatcggctc gaggatggcg atgacgataa acagcaccac gcccgccatc
     4861 atcaccacca gcgccggttc aaacagccct aacgccagcc ccacctgggt atcaaattcc
     4921 cgctcctggt tgacagcggc ctgctcaagc atggtttcca gttcgccgct ctgttcgccg
     4981 gaggcgatca tgtacagcat catcgggggg aacaggcgca actccgccag cgcggcgcgc
     5041 agactgcttc cttcgcggac gcgatctgcc gccagcagca gttgttgctc gacatagcga
     5101 tttgccgaca cagcggcagc ggtctgaatg ccttccagca gcggaacact gctggcggtg
     5161 aggatgctta atgtgcggga aaaccgcgcc gtattcagtc cgcgcgcaac acgccctgtc
     5221 acgggcaggc gcagcaaggt tttgtcccag cgcaggcgca taacaggatt tttgagtagc
     5281 cgttgcccca gcaccagaag cgccagcaat cccgccagcc agtaaacgcc gctggcctgt
     5341 aacgcgtcac tcatggcgat aagcgcgcgg gtggtggcgg gcagcgcgtg tccgaggtga
     5401 tcgaactgtt cgatgatttt cggcaccact gccgtcagca aaatagtgac cacgcccgtt
     5461 gccaccacta gcagaaccag cggatagagc atggcctgca atagacgtga tttcaggcgc
     5521 tgtcgctgtt cggtgtaatc cgccaggcga ttaagcacca cgtcgagatg cccggatttt
     5581 tctccggcag ccaccatcga acaaaacagg gaatcaaaga cgcggggatg ttcgcgcagg
     5641 ctgtccgaca gggtgtaacc ttcctgaatc cggctgcgca gcgccattcc gaggcttttt
     5701 acatgcagtt tttcactttg ctcactgacc gcctgtaagc aggtttccag cggcattgct
     5761 gcctgcacca gcgttgccag ttggcgcgtg aacagcgcca gatctgccga cgccacgcga
     5821 cgatgtgcgt gtcgccgacg ctgcaacatt ccccctgccg aagcattcat ccgggcttca
     5881 atgtgcatgg gaatgagatc tttaccgcgc aacaactggc gggcatgacg cgcggaatcc
     5941 gcctcaatca tgcctttggt tttgcgacca ttacgctcca gcgcctgata gtaaaacagt
     6001 gccattacgc ctccatggtt acccgcagaa cttcatcgag agaggtttct ccggcgagca
     6061 ctttctcaat gccgttgctg cggatgcccg cagagtgttg ccggacataa cgttccagct
     6121 ccagttcccc ggcctgacgg tggatcaaat cacgcaatgt ggcatccacc acgatcagct
     6181 catggatggc agtacgtccg cggaaacctt tatgattaca ggcggggcag ccctgcggat
     6241 ggtacagagt gacggtatgc gcgtcggtaa ttcccagcag gcgtttttct tcgtcagtgg
     6301 caggcgcggg ctggcggcag tcggggcaca gcgtgcgaac cagtcgctgc gccatcacgc
     6361 ccgtcagact ggaagagagc aggaaaggct ccacgcccat atcctgcaaa cgcgtgatcg
     6421 cccccaccgc cgtgttggta tgcagcgtgg aaaggaccag gtgtccggta agtgaagcct
     6481 ggacggcgat ttctgcggtt tcggtatcgc ggatttcacc gaccatcacc acatccgggt
     6541 cctgacgcaa aatcgcgcgc aggccacggg caaaggtcat gccgacgcgg gtgttaacct
     6601 gtgtctgacc aattccttca atcatgtatt cgatggggtc ttcaaccgtg agaatattgc
     6661 gcgaatggtt gttcagctcc tgcaatccgg cgtacagcgt ggtgcttttt ccggaacccg
     6721 tcggccccgt taccagaaag atgccgtgcg gtttgtgtaa cagctggcgc aactgcgcgg
     6781 tcagttgctg gctaagcccc agacgctcca gcgtcaggcg ggcctggttt ttgtccagca
     6841 gtcgcagcac cacgcgctcg ccccaggcgg agggcatggt ggagacacgc acgtcaatcg
     6901 cccgaccgcc cagcagcagc gcaatacgtc catcctgcgg tacgcgcttt tcggcgatat
     6961 ccagccgtgc catcaccttg atacgcgata ccagcagcga ggccagtttg cgccccgggc
     7021 gcagcatttc atgtaatgtg ccgtcaacgc gaaaacggat caccagactc ttttcaaacg
     7081 tctcgatgtg aatatccgaa gcgccttctt taatcgcctc tgccagcatg gcgttgatca
     7141 gtttgatgat cggcgcatcg tcgtcacttt ccagcagatc ttccgtttcc ggcagttctt
     7201 cagcgagagt aaaaaagtcc tcagcagaac cgagatcttc cattagctgg cgggcctcgg
     7261 aagagtcccg ctgccagacc gcattcagtc gctgttcaaa ttcggcctcg tcgatttgcc
     7321 gcagcgtaaa gggcgcgttc agcccccgct gcagctcctg caaaacagag agcgacaacg
     7381 gatgcacatg gaggatctcc agcgacgctt cgcaccatgc caccaggcta aaccgacggc
     7441 tgaaactgta gggcagacgc acggtgttag cggtggtttc ctgtgctaca ggcaccatta
     7501 acgcgttctc ccggcattaa ggaacgcacg tacttccggc ggtaaggcct ggttttgcgc
     7561 tggcagtacc ggctgcgcgg tgtgcggcat caggcttaag ccttgctcat cgcgatagat
     7621 ctgttcggcg cgcatatagt tatatttgcg ctgcgacacg ccgtctgccg ccataccgtc
     7681 acgcagaatg gtcggacgga taaacaccat caggttacgt ttttcttttt tatccgccgt
     7741 cgatttaaac aggttaccaa tcaacgggat atcgcccagc agcggcactt tcgccacact
     7801 ttctcccgcc tggtcgtcca tcagaccgcc aagcacgatc agttcaccat cgttagccag
     7861 cacggtggtc ttcagtttgc gctcaccaaa caccacgtcg aggctggtct gtccttccac
     7921 cttcgagact tcctgctcaa tcaccatctg taccgcgtta ccttcgttaa tctgcggcgt
     7981 gactttcagc atgatgccga cttttttcct ctctactgta ttgaaaggat tgctgttatt
     8041 ggagccaacg gtagagccgg ttaataccgg aacgtcctga cccaccatga agaaggcttc
     8101 ctggttgtcc agcgtggtga tgctcggcgt ggagagaacg ttcgagctgg agtcgttttt
     8161 aaccgcctgt accagcgcca tccagtcgcc tttcaccacg ccaaccgccg taccgctaaa
     8221 gccggaaaga agctgggcga gcgtggagag atcgccgttg gtatccggat taatggtggt
     8281 tgcgccgttt tcactgatca ccgtcgagcc tttctgcggt ttcgcctgag aaatggctgc
     8341 gcccagcgta ccgataggga tctgcgtacc attagcaaac tgcattaatc cggcatcttt
     8401 cgacgcccac tgcacgccga agttgatatt actgccttcg gccacttcca cgatcaacgc
     8461 ctcgacgtgc acctgagcac ggcgaatatc cagttgttca atcacgcttt gcagcgactg
     8521 catgatgtcc tgcggcgcgg ttacaatcag ggcattactg tgtttgctgg cggcgatgga
     8581 gacaacctca cgcccgctac cgaccgtgcc ttccgcctct tctttagccg ccgtgagcgt
     8641 gccgctgacc tgcttcagta catcgaccag atcttcggct ttgctgtatt tgagatagaa
     8701 aacctggctg ttgccgctgc gctccatttc tgagtccagc cgacggatca ggcggcgcat
     8761 tttgtcccgc gtggccgggt caccactgac aatcacactg ttggtgcgtt cgtcggcgac
     8821 aatttgagat ttcagcgtcg caggctggtt ctcgccgctg tttttcgtca ggctttccag
     8881 cacgcgggcg atttccgaag ccgaggcgtt atccagcggg atcacctctt cagtgcgatt
     8941 acccgcgtga tccacacgct ggatcacttc cgtcagccgc tccacgacgg aggcgcgtcc
     9001 ggtgagcata atcacgttgg agggatcgta attaacaacg ttgcctgagc ctgcgctgtc
     9061 gatcatctgg cgcagaatcg gtgccagctc gcgtaccgac acattacgta ccggcactac
     9121 tttggtgacc atttcatcgc ccgcgtagtt gtcgctgcct tcaccaacca gcggcagcgg
     9181 ctcgactttc gcggcactgg atttcaccac cttcagcacg tcgttttcca tcggtacgac
     9241 cgcatacccc tgtgcttcca gcaggttaag gaataactgg taatactggc gttcattgag
     9301 cggagtcata gtgcgaatac tcactttccc ctgaacgccc ggccccataa tgatggtttt
     9361 attaaggtta gcgccgacgg tttcgatgaa cgatttcagg tcggtatctt taaaattagc
     9421 ggtgaaagtg gcttcttccg cccagaccgg tgaactgcat aatgccgctg ccaacaccag
     9481 cggcagtaaa cgcttttttg ttttgaggcc agttgtcttc ttacgccaga ccgacaacgt
     9541 aatatcacgc caaaacacga tgaatgattc tcctggatta aatgcggtta gcgcagcgcg
     9601 atggaaatgt cgtggcgcgc acccttgcgt aaaaccgtaa gttgaatgga atccattgaa
     9661 ggtaactgcc gcatcagagc aatcattgct cgtggatcag tgaaatcctg ctgattgagc
     9721 gcaatggcga tatcgccttc cttgaaaccg ctggcatcga acagagaacg atctgccccc
     9781 ggtttcactg cataaccgac aattccctcc ttacgcacag gcgtaagctg gatatagtta
     9841 aaaattttct gcggatcttt cgccagtgcc tgacgcacgg cagtcgggat ctcaactggc
     9901 gcactgacag caggttcagc aacagcttgc tttgcttcgt cactgatggc ttttttgttg
     9961 gtcacggcta cggtggaacg ctcctcttca accaggctca ggcgctctat ttttccctga
    10021 tagcgcagca tcacatggtc gcggttgatt tcctcaatca ccgcgttgtg agaaccaagc
    10081 cgttccccct gcaaatagac ctgctgtttg ccaccttctt caataaccgc gccgggtctg
    10141 gcaccaaagg cgatcccgcg cagcaccaca ttaagacgcg tttcggccac aggtgcaggt
    10201 tcgggttgtt ttaccggtgc ggcgacgggc tgatatttgc caaaccagtt ttgctggctg
    10261 attaattgca cgtcattttt atcgaacgtt tttgcatccg tacggggcgg tttattcacc
    10321 gatggagaaa ccaccgtata ttccgcagaa aaggagaaat aacgccagag tgaatgcgcc
    10381 acttttgcag aaataataag cagcatcagc cagaacatcc cgcgtgcaat ttttcgcaga
    10441 tgctctttgt ctgtattaag agattgtctc tggttaagag tgtgacgaat ttttgtcagc
    10501 cattgaatga gataaattcg tgcgtcacga aaaacaaccc gcgccaattt atatctaccc
    10561 gacgttatgc tttgactatt ccacaggtgg tacgatccag tttccgctga tactggttac
    10621 ctgtacgtgt atcattaatc gttatgctgt aattaattcc ctcagtgagc attaatttca
    10681 ccgttgggtc agcgcacatt tgtcgttgat agctggttaa aaaggcgtct ggcgtctgcg
    10741 tgatctgagt acccgcttcg ctgataatgg tcatttttac cgtcgtgccg ctactttgtg
    10801 ccagcactaa ggtatatccc gcagatttaa tcggcaggtt ttggctgata ttttgcgcct
    10861 gttttttcgc cagtaaactg gcattttcat tatggccggc acagccactt aataatcctg
    10921 taacgctcaa caatagcgat attaataccc tccctggcat ttgttttatc gacatggtaa
    10981 ataatctcta aggttattaa taagagttaa aatgtcactt tgataatgac gtggttatca
    11041 ttaaaacaat gcctgtagat aaagtgttgc tataccgccc agacttaaac acggtccaaa
    11101 aggcagtgtg gttgatcctc tttttgtaat aacggcatat atcaggccgc agcatgaggc
    11161 gattaaagca acattgggca gtgataacgg tcccacccaa ctacctaaag cggcgaacaa
    11221 taatacatcg cccatgccta atgcttcttt acgcagaact attccggcta tccagcgcag
    11281 agagtaaaaa gtgataaacc ccaccaggac gccggtgact gcatcttgta gcgtgagcgg
    11341 gctctgctgc gcccatgccg caattagtcc cgtccacaat acgccctgag taaaaacatc
    11401 gggcagccat tggtgatcga ggtcgatgat gctcgcggca atcagccagg cggataatat
    11461 catcaccgcc agcgcccatc cactttccgg ccacaccagg ctcgccagca aaaaagcgag
    11521 tgccgtcaat aactccacca gcggataacg cttgctgatt ttcgcctgac aatcacggca
    11581 gcgccctttg agcatcagcc aggagagcag cggaatattg tcacgtatcc ggatggtctg
    11641 ctgacaatgt gggcagtgcg aacgcggtag cgcaaggctt atttttgact gcgcactcgg
    11701 catttcacca tgaaactccg ccatttgttg gcgcagcatg atggggtaac gccaaatcac
    11761 cacattcaaa aaactgccga tgatcaatcc tccgacggta gccagtatgg gcatcgccac
    11821 ggggtattgc tgaaaaacat caaaaagcat ggttaaaggt tgtttgttgt aacttgccgg
    11881 atgcggcgta aaacgcctta tccggcctac aggtatctgc caacgcaagt aatcgcggct
    11941 ttccctgaag acccgataag atggcgcatc gggcatatgt gttaacccga tgcgccttat
    12001 ttcatgccgg atgcggcgcg agcgccttat ccggcctaca ggtgtctgcc aacgcaaata
    12061 aacgcagctt tccctctctg taggcccgat aagcttgcgc atcgggcata tgtgttgacc
    12121 cggtgcgcct tatttcatgc cggatgcggc gcgagcgcct tatccggcat acaggcttac
    12181 tcggctgaca tcttatgctc ggtaacctga ttaatggttt ccggcccttg ctgcggtttc
    12241 ggcagaccaa gcgccgccag cgtctcgtaa gccgactggc tcacaccgcc ctcgaagttc
    12301 atctcgctca cccccggcag ctggtaagca ttcgcgcccg gattccattt tttaaagaac
    12361 tccgaaagat ccgtctgggc gacccaggag gcacacagca tcagcttgtc ggcagcgtta
    12421 ccgttggatt cggcacagta gtttctgttg ccaaatttgt cattgccaac atcatcgccg
    12481 cgtgctttac gatgcatcaa ctggaacagg ttccagcctt tcatcccttc acgctcgctg
    12541 taaaactctg gcagaggagt gccatctgga taccatttct tgatatcaaa gtttttctct
    12601 gcccattcct tcagctgcgc gtacatcagc aaacggtcac ccgcaccgcc gcgcgcccaa
    12661 gcctggttgt tgctctcctc cagatattcc ggcgcgacgg taatatcgtc agcgacacgg
    12721 ttcatcttgc cgagatagcg atcctgcatg tacagcgcca gcacgttgtt cgccacttcg
    12781 gtcgcgcctg gaacagtcag cggcgtttct gcggcgttgt gccctacttc gtgccagatc
    12841 aaccagtcgt tcagcggcgt cgtcggcagc gtggtgctgt tcgtcgagaa gctgctgttc
    12901 attaccggat aacccgagtg cgcatcaccg atggagatct gcacatcgtt tgtgaaacga
    12961 tgtttatgtc ccgtcaatgc tttataggta aacatccggt gcttaccgct cgtttcatca
    13021 cgaccgtaga agtcattcat cgagctggca aaggtgtcca gatcgttagc gaattgctcc
    13081 agtccgccag tgtaattgct ggcattcagg ttcttcttcg gtgtggtata gacgaacgag
    13141 gcagactcca gttcgcccag cggagccggt gagttcagat cgtttttcca tgcgccgtct
    13201 ttatagaacg gtgcttttac cacgccagta aaggtgaagc tggcagattc attggtagag
    13261 ctattgccct tgatataaat caggccaccg taaggcacct tgaacttcac cgtaccgcta
    13321 gcgtccagag agtacgtttt agtcactctt ggcggacggt tcagcgcaac ttcatgcttc
    13381 tcacgtccgg tcaggtcgtc agccagcgcc acggtgacgg tcacaggaac gttcgcattg
    13441 gacttaatgg tgacctcttt ctgagccggt gcccacaggc cggttgattg catgttaccc
    13501 gcaaaccatt tggtcggatt cgagtacagg ctgatggttt cagtaacgtt ctgtccctct
    13561 tccgatactg ctcccggata cttctccaca tcaaccttaa tgttcagatc ccaccaggaa
    13621 cggcccagca tcaggcgcgt cagcggtttt tccatatagt tgagtggata gcttgggttc
    13681 atcatgcccg ctttaccgct actttcaccg tagatcatgt tgttatcgac cagcgatttt
    13741 ttcagatctg cggagcactg tgtgccatcg gtataggcat cattggtata gcagttcagg
    13801 aactcggtga acgttttaaa gcccagctcg tcatttttgc cttcttcata acgatagctc
    13861 gtatcgttcc acagccagac cgacatgttt tggtacagac gttccagatc gacgctgctc
    13921 agacgctcac ctttgcgacc attggtccgg aagtagagct catgctgata cagacgctga
    13981 atgttggtgc ctaaatctgc agcctgcacc atcgcttttg cagtgtcggc gttaaggctt
    14041 agttgcgtat actgtggaac atacatgcca ccagtaaccg gaactcccgt gccaggacga
    14101 tattccaggc agttgacctc gtagtggtag tcagaatcct tacactcttc cagccctggg
    14161 aacgcgtcca gaattctctt cttcgccgca gccagtgact caggcgtttt gtggtcggct
    14221 tcatcgataa aggcgaattg agttacctgt ttaccctcaa cttcttcctg ccagctggca
    14281 acttccagct tcggtttgtc atcaggtttg ttttcttgct gatatttcca gataacttct
    14341 ttcgtggtgt catcaatggt atacggtggt ttaccatcga cagccggata acgctcatag
    14401 acccaaattg gcgttgaacg tcgttgacga acgcggtccg ggtagccttg cggatcatta
    14461 tttactaccg acttgttaag cgccatcgac aaacctgcgg catcaagcag acgtacaaag
    14521 ccagatgcgc tctcttcctt aagattgctc atcacgtttt ccatgatcag caccgatcca
    14581 cctttgttca tataggcgat caaatcggtc acatcctgct gagtcagctt cggtttgctg
    14641 gtatctgcac gcagcgggat tgcataagga tcattaccaa cctgagtcac atactcaaag
    14701 ccgttgagga tcagcagcgg catttcctgc gggtcgagat cgccatagct acttaaatgc
    14761 tcaacagaga tacccgcaaa atccggatga aagccgaact cagcgctgtt tcctgtaacc
    14821 tgaccatgac gtttgaaata gacagtatcc aggttggtgc ctacggtcat gctggctttc
    14881 gcgtccggcg tccatttatc gtcggacaga tagcgcagta cgttctgcat gaagtgcttc
    14941 atgtcatctg aatcaccgct gagcgtacac tgcccgtctt tattaacgcc cccgttccag
    15001 ctgtaaccgt tcgggcaacg caaaatgctg ttgtagtgcg ggttaccgat aaccatcagt
    15061 ttgccctcac cgacttgccc cagcgaaata aacggcaggt taaaggtggc ggtatcgcgc
    15121 gtaacgtttt ccggctcaac aagagaaggc gcttccgtaa tgtacgccag ctcgttttta
    15181 tcccaggcgc gtttttcgcc aaacgccagc cagtagtttt tatcattacg cgccatcaga
    15241 attgggaagg ccgcgttgga gatattcacc accgcctgac cgcgcgcatt accagtgctg
    15301 ccatagaagt tggtagagtc atgaaagacg tggaatttcg tcaccgattt gtaatcttca
    15361 tccacacccc acagcttgtt aataacgccc tgaatctggc cgtcattaac attgcgcgtc
    15421 gtcagcgaga accagcgagc ctcgttacaa ccatcggttt tcgcacaaat cgcggtatcg
    15481 atctctttgg cctgacccgt attaaactgc tcaataaatt cgtttggcag attaacaact
    15541 tgctcacctt cccccagcgt cgcaccgttg gataacgaga gattgataat ctcgttgatc
    15601 acgttgggat attcggcaaa gaccttgcgt acatcgtccg gaacaacacg ggtattattt
    15661 tgcccggtcg tcgaatagcg atgaataagc tgatcaatat tcgccccgcg aacttcatca
    15721 cccagttcag tcagcgcaat ggtcgactta ttgccgcgca ctgaacccag ttcaaaggta
    15781 tcgataccaa aggagatggt ttcgccccag ctaaaggaaa attcaccatt ttcccctgtc
    15841 acgccacggc ctgaattggt gtagtagttg acgccagcaa caccatatcc ctggctatcg
    15901 accaggcgac cttctgagag aatgatttcc gagggttgat actgataaaa ctgttccgcg
    15961 ttagccgaca cgaaggaagc gttcagatcc agttttgttc ccggcgtggt gacgggcacg
    16021 accggtgaag tatgagtgga tggcgcttta tcggtcgcag cattgttttc cacctcttca
    16081 ttgaccagct ttttgaactc ttccggtgcc agatcgattt gcttatacag cgagtcgaag
    16141 cgtttactct cgatcaccga ggagaacgtc agacaaacct gttctgtatt cgccggacag
    16201 ctgttactgg acgttaccag cgaaaccgca ttgcttttct tgtcatcgga gcccgccagt
    16261 tcttgcgcgt cctcaaggct aaacgacact ttttcaaccg cacgcaagct acgcgcagct
    16321 tctgactgag tgttgaaggt ggcaattgtc gtgttacccg ccacgcaagt aacgtcctcg
    16381 ccaggtttaa atgtaaagcc atcgctggat tcaccattac aggtagcacc agttacccgc
    16441 tggcttccgc ccagggtcag ataacccgtt ttcgtaggaa caggttctgg ctccggttct
    16501 ggtgttggtt caggatcagg tatcggttct ggcgtaggct ctgggtccgg cgttggctca
    16561 ggcgtcggct ccgggtttgg tgttggatca ggtttcactt ccggcaaaga ccctgttcca
    16621 gaatctacag gcggcgtatc ggaggaagat ccggagccac cgccatcaca accggctaac
    16681 agggttgcac tcaaaatagc cgctaa
//
LOCUS       DAZKFU020000091        16672 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZKFU020000091 DAZKFU020000000
VERSION     DAZKFU020000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.092"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..588
                     /gene="ddpX"
                     /locus_tag="ACHOQQ_RS12000"
                     /old_locus_tag="pgaptmp_002400"
     CDS             43..588
                     /gene="ddpX"
                     /locus_tag="ACHOQQ_RS12000"
                     /old_locus_tag="pgaptmp_002400"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: protein motif:HMM:NF007557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HGU4470640.1"
                     /translation="MINNGFYAKSETFGGNGRRTPIYQQARCLLHKDAITALAKSISI
                     AQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGTAIDLTLRDEHGN
                     ILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGISSEWWHFELPQAA
                     SYPLLADQFTCFISPGTQHVS"
     gene            602..2152
                     /gene="ddpA"
                     /locus_tag="ACHOQQ_RS12005"
                     /old_locus_tag="pgaptmp_002401"
     CDS             602..2152
                     /gene="ddpA"
                     /locus_tag="ACHOQQ_RS12005"
                     /old_locus_tag="pgaptmp_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4470641.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASGWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTNKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRNYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            2154..3176
                     /gene="ddpB"
                     /locus_tag="ACHOQQ_RS12010"
                     /old_locus_tag="pgaptmp_002402"
     CDS             2154..3176
                     /gene="ddpB"
                     /locus_tag="ACHOQQ_RS12010"
                     /old_locus_tag="pgaptmp_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4470642.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGDE"
     gene            3173..4069
                     /gene="ddpC"
                     /locus_tag="ACHOQQ_RS12015"
                     /old_locus_tag="pgaptmp_002403"
     CDS             3173..4069
                     /gene="ddpC"
                     /locus_tag="ACHOQQ_RS12015"
                     /old_locus_tag="pgaptmp_002403"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGU4470643.1"
                     /translation="MMLSEETSAVRPAKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCVFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            4066..5052
                     /gene="ddpD"
                     /locus_tag="ACHOQQ_RS12020"
                     /old_locus_tag="pgaptmp_002404"
     CDS             4066..5052
                     /gene="ddpD"
                     /locus_tag="ACHOQQ_RS12020"
                     /old_locus_tag="pgaptmp_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4470644.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            5045..5971
                     /gene="ddpF"
                     /locus_tag="ACHOQQ_RS12025"
                     /old_locus_tag="pgaptmp_002405"
     CDS             5045..5971
                     /gene="ddpF"
                     /locus_tag="ACHOQQ_RS12025"
                     /old_locus_tag="pgaptmp_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGU4470645.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWTLRKTELPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIRENGRELRCWRAL"
     gene            complement(6027..6458)
                     /gene="osmC"
                     /locus_tag="ACHOQQ_RS12030"
                     /old_locus_tag="pgaptmp_002406"
     CDS             complement(6027..6458)
                     /gene="osmC"
                     /locus_tag="ACHOQQ_RS12030"
                     /old_locus_tag="pgaptmp_002406"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGU4470646.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            6804..7019
                     /gene="bdm"
                     /locus_tag="ACHOQQ_RS12035"
                     /old_locus_tag="pgaptmp_002407"
     CDS             6804..7019
                     /gene="bdm"
                     /locus_tag="ACHOQQ_RS12035"
                     /old_locus_tag="pgaptmp_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGU4470647.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            7121..7258
                     /gene="sra"
                     /locus_tag="ACHOQQ_RS12040"
                     /old_locus_tag="pgaptmp_002408"
     CDS             7121..7258
                     /gene="sra"
                     /locus_tag="ACHOQQ_RS12040"
                     /old_locus_tag="pgaptmp_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGU4470648.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            7415..9112
                     /gene="maeA"
                     /locus_tag="ACHOQQ_RS12045"
                     /old_locus_tag="pgaptmp_002409"
     CDS             7415..9112
                     /gene="maeA"
                     /locus_tag="ACHOQQ_RS12045"
                     /old_locus_tag="pgaptmp_002409"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4470649.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            9246..10256
                     /gene="adhP"
                     /locus_tag="ACHOQQ_RS12050"
                     /old_locus_tag="pgaptmp_002410"
     CDS             9246..10256
                     /gene="adhP"
                     /locus_tag="ACHOQQ_RS12050"
                     /old_locus_tag="pgaptmp_002410"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGU4470650.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDV
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            10403..10687
                     /gene="yddM"
                     /locus_tag="ACHOQQ_RS12055"
                     /old_locus_tag="pgaptmp_002411"
     CDS             10403..10687
                     /gene="yddM"
                     /locus_tag="ACHOQQ_RS12055"
                     /old_locus_tag="pgaptmp_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4470651.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(10915..11568)
                     /gene="fdnI"
                     /locus_tag="ACHOQQ_RS12060"
                     /old_locus_tag="pgaptmp_002412"
     CDS             complement(10915..11568)
                     /gene="fdnI"
                     /locus_tag="ACHOQQ_RS12060"
                     /old_locus_tag="pgaptmp_002412"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGU4470652.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(11561..12445)
                     /gene="fdnH"
                     /locus_tag="ACHOQQ_RS12065"
                     /old_locus_tag="pgaptmp_002413"
     CDS             complement(11561..12445)
                     /gene="fdnH"
                     /locus_tag="ACHOQQ_RS12065"
                     /old_locus_tag="pgaptmp_002413"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGU4470653.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(12458..15505)
                     /gene="fdnG"
                     /locus_tag="ACHOQQ_RS12070"
                     /old_locus_tag="pgaptmp_002414"
     CDS             complement(12458..15505)
                     /gene="fdnG"
                     /locus_tag="ACHOQQ_RS12070"
                     /old_locus_tag="pgaptmp_002414"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(14918..14920),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4470654.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            15737..16618
                     /gene="yddG"
                     /locus_tag="ACHOQQ_RS12075"
                     /old_locus_tag="pgaptmp_002415"
     CDS             15737..16618
                     /gene="yddG"
                     /locus_tag="ACHOQQ_RS12075"
                     /old_locus_tag="pgaptmp_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGU4470655.1"
                     /translation="MTRQKATLIGLIAIILWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSMPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         3965 a         4336 c         4312 g         4059 t
ORIGIN      
        1 gtgatatcgg agatattgag tccgaatatg acggttaaca acatgataaa taatggtttt
       61 tatgctaaga gtgagacttt tggaggaaat gggcgcagaa cccctattta tcagcaagcg
      121 cgttgtctgt tacacaagga tgcgattacc gcgctggcga aaagtatcag tatcgcccag
      181 ctctcaggct tacaactggt gatttacgat gcgtaccgcc cacaacaagc acaggcgatg
      241 ttgtggcaag cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac
      301 agccgtggca cggcgatcga cctgacgctt cgtgatgagc acgggaacat cctcgatatg
      361 ggcgcagggt tcgatgaaat gcacgagcgc tcccatgcct atcacccttc cgtcccgccc
      421 gccgctcagc gcaatcgcct gttgctgaat gcgataatga ctggtggcgg ttttgtcggt
      481 atctccagcg aatggtggca cttcgaatta ccccaggcag cgagttaccc actgctcgct
      541 gatcaattca cctgttttat atcccctggc acacagcacg tcagttaatc aggaaactgt
      601 catgaaaaga tcgatatcgt ttcgtcctac attgctcgcg ctcgtccttg ccacaacttt
      661 cccggttgcg cacgccgccg taccgaaaga tatgctggtg attggtaagg ccgccgatcc
      721 acaaaccctc gacccggcgg taacaataga taataacgac tggacggtga cctacccgtc
      781 ttatcagcgg ctggttcagt acaaaacgga cggtgataaa ggctcaaccg acgttgaagg
      841 cgatctggcg agtggctgga aagcgtctga cgatcaaaaa gagtggacgt tcaccctgaa
      901 ggacaatgct aaatttgccg atggcacacc cgtcactgcc gaagcagtaa aactctcttt
      961 tgagcggtta ctaaaaatcg gccaggggcc agcagaagca tttcccaaag atttaaagat
     1021 tgatgctccc gacgaacata cggtgaagtt tacccttagc cagccatttg caccgttcct
     1081 ctacacgctg gcgaatgacg gtgcatccat tatcaatccg gcggtgttaa aggaacatgc
     1141 ggcggatgat gcccgcggct tcctcgcgca aaataccgcc ggttccggac catttatgct
     1201 gaaaagctgg caaaaaggtc agcaattagt tctggtgcca aatccgcatt accctggcaa
     1261 taaaccgaac tttaagcgag tatcggtaaa aattattggt gaaagtgcct cccgtcgcct
     1321 gcagctctcc cgtggcgaca ttgacattgc cgatgcgctg ccggtggatc aactcaacgc
     1381 cctgaagcag gaaaataaag tcaatgtggc agagtatccg tcactgcgcg tcacctatct
     1441 gtatctgaat aacagcaaag cgccacttaa tcaggcggat ctgcggcggg ccatttcctg
     1501 gtctaccgat tatcagggca tggttaacgg cattctgagt ggtaacggaa aacagatgcg
     1561 cggcccgatt ccggaaggca tgtggggcta cgatgcgacg gcaatgcaat acaaccatga
     1621 cgaaacgaaa gccaaagctg aatgggataa agtgacgaac aaacccacca gcctgacgtt
     1681 tctctattct gataatgatc cgaactggga gcctattgct ctggcgacac aatccagtct
     1741 caacaagctg ggcatcaatg tgaagctgga aaagctggcg aacgccacca tgcgcgacag
     1801 agtgggtaaa ggtgattacg acattgcgat tggcaactgg agtccggatt ttgccgaccc
     1861 gtatatgttt atgaattact ggtttgagtc agacaaaaaa ggtctgccgg gtaaccgctc
     1921 gttctatgaa aacagtgagg tcgataagtt actgcgcaat gcgctcgcga ccaccgacca
     1981 gacgcagcgt acccggaact accagcaggc acagaaaatc gtcattgatg acgctgctta
     2041 tgtgtacctg ttccagaaaa actaccaact ggcgatgaac aaagaggtga aaggctttgt
     2101 gttcaatccc atgctggaac aggtcttcaa tatcaatacc atgagtaaat aacgtgacct
     2161 tctggagtat tttacgccaa cgctgctggg ggctggtgct cgtggtggcg ggcgtctgcg
     2221 tgattacgtt tattatctcg catctgatcc ccggcgatcc ggcgcggtta ctggcgggtg
     2281 accgcgccag cgatgctatc gtggaaaata ttcgccagca actgggactg gaccagccac
     2341 tgtatgtaca gttttaccgc tacgtcagcg acctgtttca tggtgacctg ggaacatcca
     2401 ttcgtaccgg gcgtccggtg ctggaagagt tgcgtatatt tttcccggcg acgctggagc
     2461 tggctttttg tgccctgctg ctggcactct tgattggcat cccgctgggc atactctctg
     2521 cagtctggcg aaatcgctgg ctggatcatc tggtgcgaat aatggccatt accggaatct
     2581 ccacacctgc gttctggctt ggactgggcg tcattgtgct gttttatggt catctgcaaa
     2641 ttcttcccgg cggcggaagg cttgatgact ggctggatcc accaacgcac gttaccggct
     2701 tttatctgct cgatgcgctg cttgaaggca acggtgaagt attcttcaat gcgttgcaac
     2761 atctcatctt accggcatta acgctggcgt tcgttcacct gggaattgtc gctcgccaga
     2821 tccgctcagc gatgctggaa caattgagtg aagactacat tcgtaccgcc cgggccagcg
     2881 gcttgcccgg ctggtatatc gttttatgtt atgcgctacc caatgcgttg atcccatcga
     2941 ttaccgtatt gggtctggcg ctgggcgatt tgttgtatgg cgcagtgctc accgaaaccg
     3001 tttttgcctg gcccggaatg ggggcatggg tagtgacatc aatacaggcg ctcgacttcc
     3061 ctgcggtgat gggatttgcc gtcgtggttt cgtttgctta tgtgctggtc aacctggtag
     3121 tggatttgct ctatttgtgg attgatccgc gtatcggacg tggaggtgat gaatgatgct
     3181 aagcgaggaa acgtccgccg tacgcccagc aaaacaaacg cgatttaacg gtgcaaaact
     3241 ggtatggatg ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat
     3301 gctattgatg atgatttttt caccgtggct tgcgacgcat gatcccaacg ccattgattt
     3361 aaccgcccgc cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg
     3421 cgatctgttc agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtcgt
     3481 ggttgccatt gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg
     3541 acgcgcagac gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt
     3601 actgacaatg gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat
     3661 tgctattgtg cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg
     3721 ccagtatacc tatgttcagg cggcgaaaac ctttggtgcg tcgcgttggc atctgatcag
     3781 ctggcatatt ttacgtaact ccctgccgcc gctgatcgtg caggcatcgc tggatatcgg
     3841 tagcgcgatt ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag
     3901 tgctgaatgg ggcgcgatgg tagcaaatgg tcgcaactat gtactcgatc agtggtggta
     3961 ttgcgtattt ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga
     4021 tggtattcgc gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt
     4081 ctggacattc aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc
     4141 aacaatgtgt ccttgcagat taaccgcggt gaaattgtcg gtctggtggg agaatccggc
     4201 tcaggtaaat cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc
     4261 gtacatcggg ggcagatttc actgctggga gacgatgttc ttaacgctcg ggaaaaacag
     4321 cttcgtcagt ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctc
     4381 aatccgacac gtcgaatagg tcttcagatg atggacgtga tccgccatca tcaaccaata
     4441 agtcgtcggg aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat
     4501 gccgtggaag ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta
     4561 atgattgcgc tggcattctc ctgcgagccg caattgatta ttgccgacga accgactacg
     4621 gcgctggacg tcacggtaca gttgcaggta ctgcgtttgc ttaaacataa agcccgcgcc
     4681 agtggaactg cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat
     4741 agcgtttacg tgatgtatgc cggaagcgta attgaaagcg gcgggacggc agacgttatc
     4801 catcatcccc ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta
     4861 ccacgccagc cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac
     4921 ggctgcgctt ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg
     4981 ctgacagcgt gtggtgacaa caaccagcgc tgcgcctgtt ggtatccgca gcaggaggtc
     5041 ataagtgtct gacacgttat taacgttacg cgacgtccat atcaatttcc cggcccgtaa
     5101 aaactggctt ggtaaaacta cggaacatgt tcatgccatt aatggtattg atttacagat
     5161 ccgccgtggt gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc
     5221 acagctttta atgggtatgc tgcaaccgag ccacgggcaa tacatccgtt ctggctcaca
     5281 acgcattatg cagatggtgt ttcaggaccc gctctcttcg ctcaatccgc gcttaccggt
     5341 gtggcgcatc atcacagaac cgctctggat agctaagcgt agtagtgaac aacagcggcg
     5401 agcgttggca gaggagctgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct
     5461 gcctcatgcg ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc
     5521 gcagcctgac gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc
     5581 gcagatcctc aatttactgg taacgctaca ggtaaatcgc gggctgacct atgtgctgat
     5641 ctcacacaat gtctcggtga ttcgtcatat gagcgatcgg gtagcggtga tgtatctcgg
     5701 gcagattgta gaactgggcg acgcgcaaca ggtactgacg gctccggcac atccgtacac
     5761 ccgattattg ctggattccc tccccgccat tgataaaccg ctggaggaag aatggacatt
     5821 acgtaaaacg gaactgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg
     5881 ttgccctctg gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggagaacgg
     5941 acgtgagctc cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg
     6001 cgcctgacgc gtcatccggc aatgctttac gatttcaact ggtaatccag tgtaatttcc
     6061 gctttcagta cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag
     6121 gtagaggcat caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca
     6181 aaaccggcat ccactttatc cagcgacaca tcggcggtgg tatcaatcga tgttggcgtg
     6241 aatcccgctt cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg
     6301 ccaatcagtt cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac
     6361 ggctgttggt tcagcacgcc actctcggtg gatactgttc ccttcccgcg tttgatatcg
     6421 ccttcccagt gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa
     6481 aaaggaaatt atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa
     6541 atccggcaga aaatattact tctgcactta aattaaattt ccacaataga taaccgggaa
     6601 tatatagctc aagagatatt gcatcaatat cattcaactc accacaataa taatacctat
     6661 caatatcatg gggttaaaat ataaaaatca tcaaccagga ctaatcttaa caacgaagcg
     6721 gcaaatattt gccatgctga atgtgcttta taaatctgcg atccgtagca gacactataa
     6781 atacacagac acggagaatc actatgttta cttattatca ggcagaaaat tcaacagcag
     6841 aacccgctct ggttaatgcc attgagcaag gccttcgggc agagcacggt gttgtcactg
     6901 aagatgacat tctcatggag ctgaccaaat gggtggaagc ttctgataac gacatcctca
     6961 gtgatatcta ccagcaaacg atcaattatg tggtcagtgg ccagcaccct acgctttaag
     7021 atgctatgct tgatcggcaa cctaatttag gggtttagca cgtgtttctt cgctacggcg
     7081 atgttgtcct taaaactagc aacaggattg aggagttaaa atgaaatcga accgtcaggc
     7141 acgtcatatt cttggactgg accataaaat ttctaaccag cgcaaaatag ttaccgaagg
     7201 tgacaaatcc agcgtggtaa ataacccaac cggcagaaaa cgccccgctg aaaagtaatt
     7261 cataaccatc agtcctcaat gacgattaaa caccattgcc tgcgcaatgg tgtttttgtt
     7321 tttatctgct ttatacttgg ggccgacgcc ctggcggtaa agcaaagacg ataaaagcgt
     7381 gccagggatg gatattcaaa aaagagtgag tgacatggaa ccaaaaacaa aaaaacagcg
     7441 ttcgctttat atcccttacg ctggccctgt actgctggaa tttccgttgt tgaataaagg
     7501 cagtgccttc agcatggaag aacgccgtaa cttcaacctg ctggggttac tgccggaagt
     7561 ggtcgaaacc atcgaagaac aagcggaacg agcatggatc cagtatcagg gattcaaaac
     7621 cgaaatcgac aaacacatct acctgcgtaa catccaggac accaacgaaa ccctcttcta
     7681 ccgtctggta aacaatcatc ttgatgagat gatgcctgtt atttataccc caaccgtcgg
     7741 cgcagcctgt gagcgttttt ctgagatcta ccgccgttca cgcggcgtgt ttatctctta
     7801 ccagaaccgc cacaatatgg acgatattct gcaaaacgta ccaaaccata atatcaaagt
     7861 gattgtggtg actgacggtg aacgtattct ggggcttggt gaccagggca tcggcgggat
     7921 gggcattccg atcggtaaac tgtcgctcta taccgcctgt ggcggcatca gcccggcgta
     7981 tacccttccg gtggtgctgg atgtcggcac caacaaccaa cagctgctta acgatccgct
     8041 gtatatgggc tggcgtaatc cgcgtatcac tgacgacgaa tactatgaat tcgttgatga
     8101 atttatccag gctgtgaaac aacgctggcc ggacgtgctg ttgcagtttg aagacttcgc
     8161 acaaaaaaat gcgatgccgt tacttaaccg ctatcgcaat gaaatttgtt cctttaacga
     8221 tgacattcag ggcactgcgg cggtaacagt cggcaccctg atcgcagcaa gccgcgcggc
     8281 aggtggtcag ttaagcgaga aaaaaatcgt cttccttggc gcaggttcag cgggatgcgg
     8341 cattgccgaa atgatcatcg cccagaccca gcgcgaagga ttaagcgagg aagcggcgcg
     8401 gcagaaagtc tttatggtcg atcgctttgg cttgctgacc gacaagatgc cgaacctgct
     8461 gcctttccag accaaactgg tgcagaagcg cgaaaacctc agtgactggg ataccgacag
     8521 cgatgtgctg tcactgctgg atgtggtgcg caatgtaaaa ccagatattc tgattggcgt
     8581 ctcaggacag accgggctgt ttacggaaga gatcatccgt gagatgcata aacactgtcc
     8641 gcgtccgatc gtgatgccgc tgtctaaccc gacgtcacgc gtggaagcca caccgcagga
     8701 cattatcgcc tggactgaag gtaacgcgct ggtcgccacg ggcagtccgt ttaatccagt
     8761 ggtatggaaa gataaaatct accctatcgc ccagtgtaac aacgccttta ttttcccggg
     8821 catcgggctg ggtgttattg cttccggcgc gtcacgtatc accgatgaga tgctgatgtc
     8881 ggcaagtgaa acgcttgctc agtattcgcc gctggtcctg aacggcgaag gtctggtact
     8941 accggaactg aaagatattc agaaagtctc ccgcgcaatt gcgtttgcgg ttggcaaaat
     9001 ggcgcagcag caaggcgtgg cggtgaaaac gtctgccgaa gctctgcaac aggccattga
     9061 tgataatttc tggcaagccg aataccgcga ctaccgccgt acctccatct aagcctgcgc
     9121 ccggtagtga aggctaccgg gcaattttcc tctccctttt tcagatctca tccatactgg
     9181 gtagtggcga ataaatctca tttgcctcac ctgctatgca gaacatcatc cgaaaaggag
     9241 gaactatgaa ggctgcagtt gttacgaagg atcatcatgt tgacgttacg gataaaacac
     9301 tgcgctcact gaaacatggc gaagccctgc tgaaaatgga gtgttgtggt gtatgtcata
     9361 ccgatcttca tgttaagaat ggcgattttg gtgacaaaac cggcgtaatt ctgggccatg
     9421 aaggcatcgg tgtggtggca gaagtgggtc caggtgtcac ctcattaaaa ccaggcgatc
     9481 gtgccagcgt ggcgtggttc tacgaaggat gcggtcattg cgaatactgt aacagtggta
     9541 acgaaacgct ctgccgttca gttaaaaatg ccggatacag cgttgatggc gggatggcgg
     9601 aagagtgcat cgtggtcgcc gattacgcgg taaaagtgcc agatggtctg gactcggcgg
     9661 cggccagcag cattacctgt gcgggggtca ccacctacaa agccgttaaa ctgtcaaaaa
     9721 ttcgtcccgg tcagtggatt gctatctacg gtcttggcgg tctgggtaac ctcgccctgc
     9781 aatacgcgaa gaatgtcttt aacgcgaaag tgatcgccat tgatgtcaat gatgagcagt
     9841 taaaactggc aaccgaaatg ggtgcagatt tagcgattaa ctcacgcacc gaagacgtcg
     9901 ccaaaattgt gcaggagaaa accggtggcg ctcacgctgc ggtggtaaca gcagttgcta
     9961 aagctgcgtt taactcggca gttgatgctg tccgtgcagg cggtcgtgtt gtggctgtcg
    10021 gtctgccgcc ggagtctatg agcctggata tcccacgtct tgtgctggat ggtattgaag
    10081 tggtcggttc gctggtcggc acgcgccagg atttaactga agccttccag tttgccgccg
    10141 aaggtaaagt ggtgccgaaa gtcgccctgc gtccgttagc ggacatcaac accatcttta
    10201 ccgagatgga agaaggcaaa atccgtggcc gcatggtgat tgatttccgt cactaagagg
    10261 cctttgctgc gactgccagt cctggtgtcg cagcatcgca cactctccaa catgaaatgg
    10321 ctaaatggat tattgttgcg taggaaggaa caataatgat agtgttccca gtgaaggaac
    10381 actactgaga gtaaagacac tgatgaaaat ggccaatcat ccccgcccgg gggacattat
    10441 tcaggaatca ctggatgaac ttaatgtcag cctgcgcgag tttgccagag caatggaaat
    10501 tgcgccctca acggcaagcc gattgctgac cggaaaagca gctttgacgc cagaaatggc
    10561 aattaaactt tccgtggtga ttggcagttc gccgcaaatg tggctgaatc tgcaaaacgc
    10621 ctggagtctg gcagaggctg aaaaaacggt ggatgtgtcg cgattacgcc gtctggttac
    10681 gcaataaaaa atggcacctt ataaagcgcc atttgaaact gatgacaaat gcaacattgc
    10741 ctgatgcgct acgcttatca ggcctacacg ttcactgcaa tctattgtat ttgaagggtt
    10801 ttcatgccgg ataaggcatt cacgacgcat ccgggatgaa caaagcccac gttgtctaca
    10861 atatgaaaat ggcgcctttt acagcgccat tttcagctta aagttcaata gagattatat
    10921 cccttcttca ctctcttttt tcgcttctgc cttctcgatt tcacgatacc agcgcggatg
    10981 gtgtttcttc gcccagcgac ggctcacctt cccttcgatc atccctttaa tcgatccttt
    11041 cacccagaat gccatataca tatggatcag gatggcgtgg atcaggatga tacccgcagc
    11101 cgcgtggatc agcaggctgt agcgaacaac ctgcatcggg aagtactgcg caaagtacgg
    11161 acgccagata atcaccccgg tcaccagcag cacgaaaatc atgctcatga tcgaccagaa
    11221 catcatcttt tgcccggcgt tgtacttacc gacatccgcc actttgtgct cattgccttt
    11281 taatacttcg acaatgttca acagccacgg aatatctttc ttatccggga tgttgtgatg
    11341 cacaaaacgc acaaacataa acatcagtgc gacgaaaatc gcaatgccga agaacgggtg
    11401 caaaatgcgt cccatctgcg gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa
    11461 gaagaacgaa atcccggaca gcgccaccag gaagaagcaa atcaccacgg tccagtgaca
    11521 ggcgcgatca ataaatttgg tgcgcacaat cattttcgac ttactcatga tgatcctcct
    11581 cgtcatcgtc cacttcctta ttcgggccaa taccgatgta atggaaaatc aaccctgcaa
    11641 aagtggcaat aaagccagcc gctgccagcg gtttcaacgc gcctttccac aggcttaccg
    11701 aggtgtcgat cttcggatct ttcggcagac cgtgatacag ctccggctga tcggcgtgat
    11761 gcagcacgta cataacgtgc gtaccaccga ccccttccgg gttgtagacg ccagcatgtt
    11821 cgtaaccacg cgctttcagt ttcgccacgc gctgttccgc cagctccagc atctccttct
    11881 tggtgccgaa gtggatagcc ccggtcggac aggttttcac acaagccggt tcctggccga
    11941 cgctgacgcg atcgacgcag agcgtgcatt tatatacccg gttatcctct ttgttgaggc
    12001 gcggaatatt aaacggacac ccggcaatgc agtaaccaca gccgatgcag ttttccgact
    12061 ggaaatcgac aatcccgtta gcgtactgaa tgattgcacc agcagacggg cacgccttca
    12121 ggcagccggg atcttcacag tgcatacagc cgtctttacg gatcagccac tccagcttgc
    12181 cgttctgttc ggtttcgcta aagcgcatca ccgtccagga cttggcgctc agatcggcgg
    12241 ggttatcata aaccccgacg cagtgcccca cttcatcacg gatgtcgttc cactccgaac
    12301 acgccacctg acaggcttta cagccgatac aggtggaaac gtcgataagt ttcgcgactt
    12361 ctgctttgta atcacgcacc tgagaaggcg gcgtgatgga gttagttgcg gaccttttga
    12421 taatgtcctg cgtttccata gccatctgtt cgccccctta cgccttctcg atgttgacta
    12481 agaacgcttt atattccggc gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt
    12541 tagcgatata acctttacgc gcgacaccct caaagcccca gtggattgga atacccaccg
    12601 tttcaacctg ctgaccgttt acattcagcg gtttcagacg gcgcgttacc acagccaccg
    12661 cgcggataaa gccacgcttg cttgagacag tgacacgatc gccattatta atgcctttcg
    12721 ccgccgccag cgtttcgctg atttccacaa actgttccgg ctgagcaatt gcgttgagca
    12781 acgcgtgctt ggtccaggtg tggaagtgct cggtcagacg ataggtcgta cccacatacg
    12841 ggaactgctc ttttttaccc atccgcagcg cgtcttgttc atacagacga acgaccgggt
    12901 tagacaccac gttcggatgc agcgggttag tgcccagcgg cgtttcaatc ggctcgtagt
    12961 gttccgggaa cggaccttcc gccattttgt tgatggcaaa caggcgtccc atcccttccg
    13021 gctgcataat aaacggcccg gttggcgtac ccggtgcggc attgccgaag tcaggaatat
    13081 cgttacccgt ccacttgctg ccgttccact ggatcagcat ccgtttcgga tcccacggtt
    13141 taccgttgat atccgccgaa gcacggttgt acagcacgcg acggttgagc ggccacgccc
    13201 aggcccatcc cagcgtattc cccagaccgg acgggtcgga gttatcgcgg ttagccatct
    13261 ggttgccctg ctctgtccag ctaccggtgt agatccagca agaagatgcg gttgtaccgt
    13321 catcacgcag atgcgcaaag ctactcagca actgaccttt cttcgcaatc agcacgccat
    13381 tagcatcata gagatcttcc agcgcatagc cgttgttctc ttttgccact tcgtcagatt
    13441 gcggttcgtg cggctgcttg tagttccagc tcatcttcat cagcggttct acgcctttac
    13501 caccttcggc ctggtacagc tcgcgcagat gatggtaaat acccgccaga atttcgccgt
    13561 cgttacgcgc ttcgcccggc gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg
    13621 agttagcgat agaaccatct tcttcagcaa agcaggtcga aggcagacgg aatacttcag
    13681 tctgaataga cgccggatcg acatcgttcg actcgccgtg gttctgccag aaggtagagg
    13741 tttcagtcac cagcggatcg ataaccacca tgtacttcag cttgctcagg cagctcacca
    13801 ctttgttttt gtccgggaag gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga
    13861 ctttgccttc gtccatcatg ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg
    13921 gcagccagtc atagccccag ttgttctctt tctgcgcggc atcgccatag aaagatttca
    13981 tcaggctaac gaagaacttc ggatagttgc tccagtagtt cacctgatca gccagcgtcg
    14041 ctttcggcgt gttcgcttcc agatacgact gcaaatcaac ctgtttttct gacggcagcg
    14101 tcagataacc tggcaggctg gtagagagca gacctaagtc agtcagaccc tgaatgttgg
    14161 agtgaccacg caatgcattc acgccgccac cggccatacc catgttaccg agcagcaact
    14221 ggatcatcgc catagtacgg atgttctgcg cacccacggt gtgctgcgtc cagcccagcg
    14281 cgtacaggaa ggtggttgtg cgatccggtg cgctggtgga ggccagcact tcacacactt
    14341 tcaggaagtc agcttttggc gtaccacaga tgttttcaac aacgtccggc gtgtagcggg
    14401 aaacgtgcgc tttcagcagg ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt
    14461 tcgcatagcc gttttcatcg aactgatagt tccaggacga tttatcgtat tgccgttttt
    14521 cagcgtcgta gccgctgaac agaccgtctt cgaaagcaaa atcatcacgc accagcaagc
    14581 tggcgttggt gtaatgctta acgtattcgg cgttgatttt gttgttttcg atcaggtagc
    14641 gcaaaacgcc agacaggaac gtaatgtccg taccggaacg aataggtgcg taaatatccg
    14701 ctacagaagc ggtacgcgta aaacggggat cgacaacgat caaggttgca tcgttgttgt
    14761 ttttcgcttc catcgcccag cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca
    14821 ttaccattac gacgttagcg tttttgatat ccacccagtg gttggtcatc gcaccgcgac
    14881 caaatgttgg agcaagactt gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta
    14941 ccgccagcat cccgagggag cgggcaaatt tctgggtcag catcccggtt tcgttgctgg
    15001 caccggaagc acacagcata ccggtagaaa gccaacggtt taccgttacg ccctgctcgt
    15061 tcttttcaat aaagttagcg tcacggtcag ctttcatcag cttcgcaata cgggagaatg
    15121 cttcttccca gctaatgcgc tgccatttgt cagaacctgg cgcacgatat tccgggtagc
    15181 gcagacggtt ttcgctgttg acgtaatcca gcaaaccggc ccctttcggg cacagcgcac
    15241 cacggcttac cggatgatcc ggatcacctt caatgtgata aatcgcttct ctggcgtttt
    15301 tcgcgccatc acccaggcta tacatcaata gcccgcaacc tacggaacag tatgtgcagg
    15361 tgttacggat ctctttagcg cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct
    15421 tcggggcaaa gcccaatgcc gctactgttg ttccagccat accgcccgcg cagattttaa
    15481 aaaattgtct gcgactgacg tccattgctt tcctcttttt tcaggggtat tactgcgcga
    15541 ggaaactaac agtaaaattc tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc
    15601 cacaaaataa tccgcctgcg caaattgacc tacctcaata gcggtagaaa aacgcaccac
    15661 tgcctgacag gccagttaaa aaaatgctat aaaattcagc ttaattttta acggcaaaag
    15721 agacaaaaca gcgagcatga cacgacaaaa agcaacgctc atagggctga tagcgatcat
    15781 cctgtggagc acgatggtag gattgattcg cggtgtcagt gaggggctcg gcccggtcgg
    15841 cggcgcagct gctatctatt cattaagcgg gctgctgtta atcttcacgg ttggatttcc
    15901 gcgtattcgg caaatcccga aaggctattt actcgccggg agtctgttat tcgtcagcta
    15961 tgaaatctgt ctggcgcttt ccttagggta tgcggcgacc cgtcatcagg cgattgaagt
    16021 gggtatggtg aactatctgt ggcccagcct gacaattctc tttgccattc tgtttaatgg
    16081 tcagaaaacc aactggctga ttgtacctgg attattatta gccctcgtcg gcgtctgctg
    16141 ggtgttaggc ggtgacaatg ggttacatta tgatgaaatc atcaataata tcaccaccag
    16201 cccattgagt tatttcctgg cgttcattgg tgcgtttatc tgggcagcct actgcacagt
    16261 aacgaataaa tacgcacgcg gatttaatgg aattaccgtt tttgtcctgc taacgggagc
    16321 aagtctgtgg gtttactatt ttctcacgcc acaaccagaa atggtattta gcatgcccgt
    16381 catgattaaa ctcatctctg cggcatttac cttaggattt gcttatgctg catggaatgt
    16441 cggtatactg catggcaacg tcaccataat ggcggtaggt tcgtatttta cgcccgtact
    16501 ttcctcagcg cttgcagccg tgctgctcag tgccccgctg tcgttctcgt tctggcaagg
    16561 cgcgctgatg gtctgcggcg gttccctgct ctgctggctg gcgacacgtc gtggctaaaa
    16621 taattgccgg gtctacggcc cggcaattaa tcagataaac cctgacaata tt
//
LOCUS       DAZKFU020000092        16487 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZKFU020000092 DAZKFU020000000
VERSION     DAZKFU020000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.093"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(53..205)
                     /locus_tag="ACHOQQ_RS12080"
                     /old_locus_tag="pgaptmp_002416"
     CDS             complement(53..205)
                     /locus_tag="ACHOQQ_RS12080"
                     /old_locus_tag="pgaptmp_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4470656.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(470..1204)
                     /gene="cysH"
                     /locus_tag="ACHOQQ_RS12085"
                     /old_locus_tag="pgaptmp_002417"
     CDS             complement(470..1204)
                     /gene="cysH"
                     /locus_tag="ACHOQQ_RS12085"
                     /old_locus_tag="pgaptmp_002417"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4470657.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1278..2990)
                     /gene="cysI"
                     /locus_tag="ACHOQQ_RS12090"
                     /old_locus_tag="pgaptmp_002418"
     CDS             complement(1278..2990)
                     /gene="cysI"
                     /locus_tag="ACHOQQ_RS12090"
                     /old_locus_tag="pgaptmp_002418"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGU4470658.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2990..4789)
                     /gene="cysJ"
                     /locus_tag="ACHOQQ_RS12095"
                     /old_locus_tag="pgaptmp_002419"
     CDS             complement(2990..4789)
                     /gene="cysJ"
                     /locus_tag="ACHOQQ_RS12095"
                     /old_locus_tag="pgaptmp_002419"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGU4470659.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAEKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVESEVHVTVGVVRYDIEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5108..5470
                     /gene="queD"
                     /locus_tag="ACHOQQ_RS12100"
                     /old_locus_tag="pgaptmp_002420"
     CDS             5108..5470
                     /gene="queD"
                     /locus_tag="ACHOQQ_RS12100"
                     /old_locus_tag="pgaptmp_002420"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGU4470660.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5548..6819
                     /gene="ygcN"
                     /locus_tag="ACHOQQ_RS12105"
                     /old_locus_tag="pgaptmp_002421"
     CDS             5548..6819
                     /gene="ygcN"
                     /locus_tag="ACHOQQ_RS12105"
                     /old_locus_tag="pgaptmp_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4470661.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDFPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTYPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHCE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYWAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6810..7070
                     /gene="ygcO"
                     /locus_tag="ACHOQQ_RS12110"
                     /old_locus_tag="pgaptmp_002422"
     CDS             6810..7070
                     /gene="ygcO"
                     /locus_tag="ACHOQQ_RS12110"
                     /old_locus_tag="pgaptmp_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4470662.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7087..7662
                     /gene="ygcP"
                     /locus_tag="ACHOQQ_RS12115"
                     /old_locus_tag="pgaptmp_002423"
     CDS             7087..7662
                     /gene="ygcP"
                     /locus_tag="ACHOQQ_RS12115"
                     /old_locus_tag="pgaptmp_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGU4470663.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7810..8670)
                     /gene="ygcQ"
                     /locus_tag="ACHOQQ_RS12120"
                     /old_locus_tag="pgaptmp_002424"
     CDS             complement(7810..8670)
                     /gene="ygcQ"
                     /locus_tag="ACHOQQ_RS12120"
                     /old_locus_tag="pgaptmp_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4470664.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTISVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIDADCQ"
     gene            complement(8667..9446)
                     /gene="ygcR"
                     /locus_tag="ACHOQQ_RS12125"
                     /old_locus_tag="pgaptmp_002425"
     CDS             complement(8667..9446)
                     /gene="ygcR"
                     /locus_tag="ACHOQQ_RS12125"
                     /old_locus_tag="pgaptmp_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGU4470665.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAAELRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCRQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9424..10833)
                     /gene="ygcS"
                     /locus_tag="ACHOQQ_RS12130"
                     /old_locus_tag="pgaptmp_002426"
     CDS             complement(9424..10833)
                     /gene="ygcS"
                     /locus_tag="ACHOQQ_RS12130"
                     /old_locus_tag="pgaptmp_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4470666.1"
                     /translation="MMGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGVLLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLILAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10855..12309)
                     /gene="ygcU"
                     /locus_tag="ACHOQQ_RS12135"
                     /old_locus_tag="pgaptmp_002427"
     CDS             complement(10855..12309)
                     /gene="ygcU"
                     /locus_tag="ACHOQQ_RS12135"
                     /old_locus_tag="pgaptmp_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGU4470667.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12379..13164)
                     /gene="ygcW"
                     /locus_tag="ACHOQQ_RS12140"
                     /old_locus_tag="pgaptmp_002428"
     CDS             complement(12379..13164)
                     /gene="ygcW"
                     /locus_tag="ACHOQQ_RS12140"
                     /old_locus_tag="pgaptmp_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4470668.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13483..14760
                     /gene="yqcE"
                     /locus_tag="ACHOQQ_RS12145"
                     /old_locus_tag="pgaptmp_002429"
     CDS             13483..14760
                     /gene="yqcE"
                     /locus_tag="ACHOQQ_RS12145"
                     /old_locus_tag="pgaptmp_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGU4470669.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVLQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADIAPTTANSK"
     gene            14787..16265
                     /gene="ygcE"
                     /locus_tag="ACHOQQ_RS12150"
                     /old_locus_tag="pgaptmp_002430"
     CDS             14787..16265
                     /gene="ygcE"
                     /locus_tag="ACHOQQ_RS12150"
                     /old_locus_tag="pgaptmp_002430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGU4470670.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLMHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     NAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3901 a         4360 c         4154 g         4072 t
ORIGIN      
        1 ggagaccaaa ccatcacaat tcagtacgcg gggttttacc cccgcgtggt ggctacttct
       61 tcgattcgta agcgagaact gccttaaact ccataccacg ttctctgata ctcaactcac
      121 acaacgagtc gcctaccatc agcgtgaatc ctagtactgt gcaacacagt acgatgattg
      181 ccacaagggc atattttgtc agcatgtctt gcctccctgc aaagaagaga ctaacattcg
      241 aattgtttgg gttcgaacgt aagcctcagg ttgatagaaa tatcgcctgg ggcttttgtc
      301 catctggaac ctcgcgaatg cttaacgcca gacagcctca agcacccgac gccattctat
      361 acctgataat tcttccagcg gcgttcacgt ttaaaatcag gaatgctgct cgcaaaataa
      421 aaacgccccc atcatttctg acagaggcgt ttaatttatc cggcaatatt tacccttcgt
      481 gtaacccaca ttcccttttt aagccaaaga aacgcgtttc ttcttccgcc atgccgggtt
      541 cccatttacg ggttgtatgg gtatcgccca ccgataaata tccttcgtcc cataatgggt
      601 gatatttcag gccatgcttt tgcagatact gatatatcgt tcggttatcc cagtcgataa
      661 tcggcagcac tttaaatacg ccacgctgaa ttgccagcac cggtaaattg gcgcggctgc
      721 cggattgttc gcggcgcagg ccagcaaacc acgtttgcgc gttaagttct ttcagcgccc
      781 ggttcatcgg ttcgactttg ttgatgtcat tgtacttttc aatgccttca acgccctgtt
      841 cccacagttt tccgtagcgt gcttcctgcc aggctgcgct ttcggtagca cggtacactt
      901 tcaggttgag cttgagtttg tccgttaact cgtcaataaa gcggtaggtt tccgggaaca
      961 gataacccgt atcggtgagg atcaccggga tatccgggcg aatctgattc accagatgca
     1021 ggctgaccgc cgcctgaatg ccaaagctgg aagaaagcac atattcaccg ggcagattat
     1081 ccagcgccca ggctacgcgg ccttcagcgt ccagtttttc cagttcggcg ttagtttctg
     1141 ccagcgccag aatgcgatct accttcggca gttcgttcag ggcgtttaga tcgagtttgg
     1201 acatagattc ctcactgttt gccttgcctg atgcgacgct cacgcgtctt atcaggccta
     1261 caagaccggg ctgatggtta atcccacaaa tcgcgcgccg gatcgagcac cgggcgaatg
     1321 atgcccgcac gcaccgtaaa gtcgccgaag ccttcacccg cttcgcgctc tttcgcccag
     1381 cgccctatca gttcatcaag cgacgccagg atttccggct cggtgatgtt ttctttatac
     1441 atccgtggga tacgtgtccc aatacggttg ccgccaagat gcaggttgta gcgacccggc
     1501 gctttaccca ccaggcccac ttccgccagc atcgcgcgac cacaaccgtt cgggcagcct
     1561 gttacacgca tcacgatatg ctcatcgctg acaccatgtt tcgccattaa attatcgatg
     1621 ttgtcgataa aagacggcag gaaacgctcc gcttccgcca tcgccagcgg gcaagtcggg
     1681 aacgagacgc aggccattga gttttcacgc tgcggcgtga cggcattcat taacccgctc
     1741 tctttggcga tcttctcgat cttcgctttc tcgctttccg gcacaccggc gatgatcaga
     1801 ttctggttcg ccgtaatgcg gaaatcacct ttgtggatct tcgcgatctc cagcaggccg
     1861 gttttcagcg gacgccccgg ataatcaagg atgcgaccat tttcgataaa cagcgtcagg
     1921 tgccagttat catcaatgcc cttaacccag ccaatacgat ctccgcgtcc ggtaaactca
     1981 tacgggcgaa tcggttcaaa tttgatcccc gcgcgacgct ccacttccgc tttaaacgtc
     2041 tcaaccccca cgcgctccag cgtgtatttg gttttggcat ttttacgatc ggttcggtta
     2101 ccccagtcac gctgagttgt cacgacggct tccgccaccg ccagcgtatg ctccagcggc
     2161 agatagccaa actcactcgc cgtgcgggcg taggttttct tgttgccgtg ttcgatagaa
     2221 agcccaccgc ccaccaacag gttaaagccc accagcttgc cgttttcggc gatcgccacg
     2281 aagttcatgt cgttggcgtg cagatcgata tcgttctgtg gcgggatcac taccgtggtt
     2341 ttgaatttac gcggcaggta ggtctggccg aggatcggtt cttcatcagt ggtggcgact
     2401 ttttcctgat cgagccagat ctccgcatac gcgcgggtac gcggcagcag atgttcagag
     2461 attttcttcg cccactcgta cgcttctgcg tgcagctgcg actcgtaagg gttcgaggtg
     2521 cagagtacgt tacggttcat gtcgttagcg gtcgccagcg catcaagacc gaccgagtgc
     2581 agcatctggt gcaccggttt gacgttcttt ttcagaatgc cgtggaactg gaatgtctgg
     2641 cggttggtca ggcgaatgct gccatagatg gtgttttcac cggcaaattt atcgatcgcc
     2701 tgccactgtt tggtggtgat aactccaccc ggcagacggc agcgcaacag catcgcgtgg
     2761 cgcggctcca gcttctgttc agcacgttcg gcgcggatgt cgcggtcatc ctgctgatac
     2821 atgccgtgga agcggatcag caggaagtta tcgcccttaa agccgccggt cagaccgtcg
     2881 tttaaatctt ccgcaatggt gccgcgcagg tagttgcttt caagcttcat gcgctcggcg
     2941 tctgtcagtt ttccttcgac cactaaaggc cctggatgtt tttcgctcat tagtagacat
     3001 ctcgctgata acggcgctct acgcgcagct cacttaaaaa ttcatccgcc gcttcggtgt
     3061 ccatgccacc aaattcggca atcacttcca gaagtgcctg ctcaacgtct ttcgccatgc
     3121 gattagcgtc gccgcagaca taaatgtggg caccgtcatt gatccagcgc cacagctccg
     3181 cgccctgttc gcgcagtttg tcttgtacgt aaactttttc tttttgatcg cgcgaccagg
     3241 cgagatcgat ccgcgtcagc acaccctctt tgacgtagcg ctgccactcc acctgataca
     3301 ggaagtcttc cgtaaaatgc ggattgccaa agaacagcca gtttttacct ggcgcttcgt
     3361 cagcggcgcg ttgctgcata aaggcgcgga acggcgcaat accggtgcct gggcctatca
     3421 taatcaccgg ggtttccggg ttagcgggca gacggaagtt gtcgttatgt tcgataaaga
     3481 cgcggacttc accctcttct tccacgcggt ccgcgaggaa gctggaggca ccacccgcac
     3541 gggcgcggcc ttcaatgtca taacgaacga caccaacggt gacgtgcact tcactttcca
     3601 cttcagcctg cgatgaggcg atggaataga ggcgcggcgt cagcgggcgc agcaggttaa
     3661 tcagcgcttc agcatcaagc tgtgccgggg agaaacgcac catgtcaaca atcggcgtcg
     3721 tcgcggcgta atgctgtaac ttcgctttat cgcccaccag cggcagcagt gtttcactgc
     3781 gggtaagcgt ggcgtaattc tcaacaatgt tggcggtgtt gacggtcagt tcgaagtgcc
     3841 actgtagcgc ttcgttcaga ggcaacgttt tgccctcgac ggtgacaggt tcatcgcctt
     3901 tcagccacag cagttcgaca agttctttca ccagtgccgg atcgttctga taccagacac
     3961 ccagcgcgtc acccggctgg taacgcaggc ccgagtcacc taagtcaatt tcgatatggc
     4021 gaacgtcttt ttcagagtta cgcccggtaa ttttctggtt aaccgaaagg ctcgctacca
     4081 gcggcgcgtc tttgctgtac gggctggtgt ggatttcatt taccacgcca gtagcgacgg
     4141 attgcgaagg tgccgcgaca ggcgcacgcg atttaagcgc atcaaccacg cgggcgcgcc
     4201 actcgctggc agcagcctgg tattcaacgt cggcgtcgac acggtcgagc aggcgttcac
     4261 cgcccagttc cgccagcttg ctgtcgaaat ctttcccgga ctggcagaaa aattcataag
     4321 aactatcgcc gaggctaaac acggcaaaag cggtattttc cagcttcggc gcttttttgg
     4381 agaacaggaa cttatgcagc gcgacggctt cttccggcgg ttccccttcc ccttgcgttg
     4441 acgtcactac gatgagcagt ttttcgctgg cgatttgttt gaatttatag tcgcccgcgt
     4501 tcaccagctt aacgttcagt ttttctgcta ataaatcatc gcgtaatgct tcagcaaccc
     4561 ggcgcgcatt gccggtttgc gaagcagaga taatagttat acccggcatt tctgcggctg
     4621 gcgctggcgt cgctgcaagc gcagcaggct gctgattgag cacgccccag aaatagccag
     4681 aaacccaggc aagctgggtg ggagttaaat cggtcgtggc cgcctgaagg cgtgccagtt
     4741 gctccgggtt caacggaagc aacgcggaag gtgggacctg tgtcgtcatg cgtcgttatg
     4801 ttccagtaag caaagctgtt tctgcgccct gtcagcgccc ataaaacaga agagaaggta
     4861 aggttaacgg ggtgaacggt gtggattaaa gacgggatag cgataactaa taaccaaatc
     4921 gactaacctg ttttagcaat agtctttaac aacaaaatag attaaacaac ctaatgaaaa
     4981 acaaatgaat ttagccaatc attaagataa atcagcgatt ttgcgcaaca aagtcgtttt
     5041 agataatgcg aaaaaacagc ctttccggta ctctacggcg gttttatcgt cctgtagaga
     5101 aattatgatg tccaccacgt tatttaaaga tttcaccttc gaagccgctc accgcttacc
     5161 acacgtcccg gaagggcata aatgtggtcg cctgcacggg cattccttta tggtgcgact
     5221 ggaaattacc ggggaagtcg atccgcatac gggctggatt atcgatttcg ctgaactgaa
     5281 agcggcgttt aagccaactt atgagcgcct cgatcaccat tatctcaatg atattccagg
     5341 tctggaaaac ccgaccagcg aagttttagc aaaatggatt tgggatcagg ttaaacccgt
     5401 tgtgccgctg ttaagtgcgg tgatggtaaa agaaacctgc accgctggtt gtatctatcg
     5461 cggcgaatga taagagtgtg tcggcggtca atttccctta agtaacgcta tgttagggtg
     5521 ttgtgttcag gatatctggg gcatgacatg gaagacgact gcgacattat tattattggt
     5581 gccggtattg caggcaccgc ttgcgcgtta cgctgcgcgc gagcgggttt atccgttttg
     5641 ttactggaac gcgctgaaat ccccggcagc aaaaatcttt ccggcgggcg gttatatacc
     5701 catgcactcg cggaactcct ccctcagttt catctgaccg cgcctcttga acgacgcatc
     5761 actcacgaaa gcctttccct gttaacgccg gatggcgcaa cgacgttttc cagcttacag
     5821 cccggcggtg aatcctggag tgtattacgt gcacggttcg atccgtggct ggttgccgaa
     5881 gccgaaaaag aaggtgtcga atgtatcccc ggagcgacgg tggatgcact gtatgaagaa
     5941 aatggcagag tgtgtggtgt catttgtggt gacgatattc tccgcgcccg ttatgtggtg
     6001 ctggcagaag gtgccaacag cgtcctggct gaacgtcacg gattagtgac tcgtcctgct
     6061 ggcgaagcga tggcgttggg gatcaaagaa gtgctgtcgc tggaaacatc cgctattgaa
     6121 gaacgttttc atctggagaa taacgaaggc gcagcgttgc tgttcagcgg cggaatctgt
     6181 gatgactttc ccggcggcgc atttctttat actaatcaac aaacgctctc gttagggatt
     6241 gtttgcccgc tctcttccct tacgcaaagt cgtgttccgg caagcgagct gctggctcgc
     6301 tttaaaacgt atccggcagt gcgcccgctt atcaaaaaca cggaatcact ggagtatggt
     6361 gcgcatctgg tgccagaagg tggcttgcac agtatgccag tgcaatacgc cggtaacggc
     6421 tggctgctgg tgggcgatgc gttacgcagt tgcgtcaata ccggaatctc cgtgcgcggc
     6481 atggatatgg cgctgactgg cgcacaggcg gcggcacaaa cgctgataag cgcctgccag
     6541 cactgcgagc cgcaaaatct gtttccgctt tatcatcaca acgtcgagcg cagcctgctg
     6601 tgggatgttc tacagcgtta tcagcatgtt ccggcgcttt tgcaacgccc tggctggtat
     6661 tgggcgtggc ctgcgttaat gcaggatatt tcccgcgatt tatgggatca gggtgataaa
     6721 cctgttccac cgctgcgcca gttattctgg cgtcatttac gtcgtcacgg cctgtggcat
     6781 ctggcgggcg acgttatcag gagtctgcga tgtctgtagc ccgtaatctc tggcgcgctg
     6841 ctgatgcgcc acatattgtt ccggctgact ccgttgagcg ccagacggca gaacggttga
     6901 ttaacgcctg tccggcaggt cttttttcgc tcacaccgga aggtgactta cgtattgact
     6961 atcgcagttg cctggagtgt ggcacctgcc gtttgctgtg cgacgaatca acactacaac
     7021 agtggcgcta tccgccttcc ggattcggca tcacctaccg ttttggataa ataataagga
     7081 taatttatgc ccctcttaca cctgctccgc cagaatccgg tgattgctgc cgttaaagat
     7141 aatgccagcc tgcaactggc aatcgactct gaatgccagt ttatttccgt gttgtacggc
     7201 aatatctgca ccatcagtaa tatcgttaaa aagattaaga acgccgggaa atatgctttt
     7261 attcatgttg atttgctgga aggcgcgtca aataaagaag tcgtgattca gtttttgaaa
     7321 ctggtgaccg aggcggacgg cattatcagt accaaagcct caatgttgaa agcggcaaga
     7381 gcggaaggtt ttttctgtat tcatcgcctg tttattgttg attcgatttc gtttcacaac
     7441 attgataagc aagttgcgca atcgaatccg gattgtattg agatcctgcc aggctgcatg
     7501 cccaaagtgc tgggctgggt gacggaaaaa atccgccaac cgctgattgc tggcgggctg
     7561 gtatgcgatg aagaagatgc gcgtaatgcg attaacgcgg gtgtcgtggc gctttccacc
     7621 acgaataccg gagtctggac gttagcgaaa aaattgcttt gacgggataa tcgtaaccaa
     7681 ttgaatttgg tttgatttgt aggccgcacg ccacatccga cattcagcgc ctgatgcgac
     7741 gcttaacgcg tcttatcagg cctacaggtc cccagtccag aaccgtaggc aggatcatcc
     7801 ggcaacacgt tattggcaat ctgcgtcaat gtttgtcacc agtgcctcca gcaccacttt
     7861 ccagtcatca accacgccaa catctgcctg tgaaaatacc gctgcgctgg cgtcatgatt
     7921 aatcgccacc acaaacttgc tattgcgtac gcccgccatt aacgctgctg cgccagaagc
     7981 gccaacaaca atacacacct caggtgccag cagatgtccg gaaataccaa tcactttttc
     8041 agcatcaacg ccaccgttca tcactcgtgc ccgactgtag cctacttcgg ctcccagctt
     8101 ttctgccagc atagcaattt cctggttatc tgcctctccg ccctgcccca ccaccagcac
     8161 ccgtctggcc tcagctaatg ggtcgcgagt aacatttttc agatcctctg tgctaaccag
     8221 ccagtccgga agcgcacccg ggacaatgtt cagttgttgc attccagagg gcaacgtggc
     8281 gtttttcgct gctcccgcct gacgcgccag ggaaaggcat agcgggcgtt tttctgtttg
     8341 cagcgtcgcc gttagcgcat ttccccagtg ggatttgcgc acgcttatgg ttggaatatc
     8401 taacgaagtc acctggcaga tgcttgcacc atgtaaacgc caggccagcc gggtcgacag
     8461 ttcatcgcca aacgttcccg gcggaaagag aactacgtcc gcaggcgttc gctgccactg
     8521 ttccaccaac gcatccagca cctgttccgc cactacaggc tgaggttcaa tctgccagtg
     8581 cgccagggta caaccgctga aatcctgtgc cgccagccag cttgctattg cggcattctc
     8641 ctgatttatt gtcacaattg cgatattcat ggctgcatcc tttggcgtaa atagtcctgc
     8701 catagctttt gcgctttttc tgctaccgtt tgcccatcaa tcagcgtcgc tccacgtcgt
     8761 tgctctgctc ttgccagttg ccgacactgc atcgctggca tttcggcagc aacagtttta
     8821 cggataattt ctgctttccc cgcagccatt cgctggcgca ttcccggtac tggcaaggcc
     8881 acttcaccgc actgacgcac ggcaatcacc gcaggcaagc gaacccggca acaacgcaac
     8941 ccatgttcag tacgctgttc gagggtgata aacagcgcgt cgagagtgaa acgctccacc
     9001 tgggtgaagc agggccagcc cagcatttcc gccagcaaaa atggcgtttg cccattctgc
     9061 ccttcgctgc tttggcagcc ggtaattatc agatccagcg gattctgacg ctgccattcg
     9121 gcaatatggc gagcgacaaa ttccggtgca aagcgcagtt ctgccgccgt ctccagcaat
     9181 acagcttcct caaacccaag agccatgaga tagcgcagcc agtgcaacgc ccgttcatcc
     9241 cccatgctca acgcggttaa agacatcggc gtaccgtttt ttctctgcgc caacagcagc
     9301 gcggcggcag cctgttcatc agcaccgagt aaacttcgca gtagcgaaac atccggtccg
     9361 ctattaccct gagccgccgc ctgccactct ttttccgcca gcattccggc atccggttcg
     9421 gctttaaacg ctaacagaat gttcattcgc acctcctaca tttcccgccg ccaccagcgg
     9481 gagtgctttg gtttctggtg cccatagcca ggtcacgaca aaaccaacta acaacactgc
     9541 cgccagaatc aataaggtaa cctgcattcc ccactgcgcc agcacccacg gcagcaggcc
     9601 agtacttacc gccgcgccaa gtcgactcat ggcggtggca aaaccgacgc ccagcgagcg
     9661 aatgtcagta ggaaaacttt ccgcaggcaa aatgcccacc agattactga ctgccgaaat
     9721 ggtggtgctg aagagaacaa aaagcagcag cgttaatgaa ctgccggaag gcaaacaggc
     9781 cattactacc agcgttgccg ccagcagcaa aaaacttccc agcaaaaatt tgcgatgtgc
     9841 cagcaggtgc gtcagaacta atcccagcag cgcgcccaca attaacaacg cattaagcat
     9901 caggctggca gtcagcgcat cttccagacc aatagtctgg gcgatagttg gcagccaggt
     9961 ataaatcaca aaccatggga ttacgaggca gacaaagaag acgctgttaa acgccgtgcg
    10021 ccgccagtaa cgcgaagaga acaaggtttt gatgtgttta tgggtcgccg ttaccacttc
    10081 atcgcccagt aaaacatggg gaccaaaata gcgatgcacg atagcgtgag cttctgcaaa
    10141 acgcccctgg cgcagtagcc agcgtggcga ttctggcgtt ccccagcgta ataacgtaat
    10201 caacaacgcg ggcagagctg ccgatgccag cagccagcgc caggcctccg ggttttcgga
    10261 aataaagtga tgtccggcaa tacttgccag cacatagcct acggtccaca ccacgctgaa
    10321 tgcgcccagc aaaacaccgc gatggcggcg cggggaaaat tcagccagca aggtgtgacc
    10381 tactgagtaa tcgcctccca gaccaatgcc gatcaaaatg cgcagcccaa taagatgctc
    10441 tggcgtggtg gcaaaaaatt gcaggaacga agcgagcgta atcagcaaaa agctgaaggt
    10501 gaagattttt tgccgaccaa tatggtcgga gatccaccca agaaccaggc tgccaagaaa
    10561 caaaccaagg agcgccgagc cgccgatcat tcccgccata aacggcgtca gttgcatggc
    10621 gggcgtaagt tgaataatgg cgtaaccaat gacgccgaga acataaccgt cggtcaggtg
    10681 tgcgccgaaa gtgagcgcag caattcgaca gtgaaaacgg ttaagcggta aatcatccat
    10741 tcgcaccggt gaagtgttca tctttacccc ttcattgtcc ggtaataaga acggcttctc
    10801 gcctgagaag ccgaaaaggc aacgccccat catcgcggaa cgctgcctga cacattattt
    10861 ttcaatcgga tagatagtac ccgtattcat aatgccgtta ggatcgaatt gctttttcag
    10921 cccttccagc aacgcccacg cgctgccgtg ttccagcttg ctccagtgaa cgcgatgttt
    10981 accaatgccg tggtggtgca ccatcgaacc gccgaggcga atggtttctt cgcagatgat
    11041 tttgttgagc ggattgtggt acttgtcgat ttcctcttcc ggcttacagt tgacaacgtt
    11101 gtagtcgtag acgaagtaca tgttggtgcc gttctgatag ctatgagagg agtgaccacc
    11161 cagcatagtg atgtcgtcgg cgtgcgggaa ctcggtacga atacggttaa taacgctttc
    11221 gtagatttcg tggatgcagc tccagcagcc ggacacttca gtggtaaagc ccatgttgcc
    11281 ggttttgagg atttgcacac gttcggcagc cactttatcc ggtccccagt tcaggttgtt
    11341 aaaccaggtt tcgatcagct tgctgtccac gcgttggcat tgcgggtagc gggcaacgat
    11401 ttccgcaatc ccttcgcccg tcgccttcgc aatgcgaggg ttaccttcag ccataaagat
    11461 cagcacgcat tttccgtcgg caaaatgggt gaagtgttgg gtgccatctt cagcgtcata
    11521 caaacgagcg atcgacggac gatacccttc caccatgatt tcacgcagga tgttgaagcc
    11581 ggttttcatg tcttccagga tatagccgta gaagaggttg ttttccgggg tgaatttaaa
    11641 gattttcact gttacttcag tgatatagca caatgcacct tcgttgccga tgatgatgtg
    11701 acgaatgtcc gggccagccg cgcggcgtgg cacgttttta atacgtgtga cggtgccatc
    11761 agccaatacg gcttccagac caacgaccat atcttcgatt gcgccgtaga gtgtggagaa
    11821 ctgcccgata ctgcgggttg ctaccaggcc gcccatctgc gccagcggct ttgactgcgg
    11881 agaatgcccc gtggtgtaac ctttttcacg caacgcgttt tccagcacct ccagcggaac
    11941 gccacattgc gccgtcgcct gcatattctc aatatcaata ttaatgattt gattcatggc
    12001 ggaaccgtcg agcaccaccg agttttctac aacagtttcc agcccacctt cggtggcgga
    12061 agcaccggta cgcggcacac cgttaatttt gtgcgcattc ataaaattca gcacacggga
    12121 tacttgctct gtggaaccga gttttacgac cgctgccgga atcggcaaag tataaatgcc
    12181 atgaatatcc ggaaatttac gaaaacggtc aatactgttc ttctttaata ctgtttcatc
    12241 ggtaattacg cgatctgcac caacaatttc ctttagctgg tcgacaatcg ctgcgcgaga
    12301 taaagacata gtaattcctt cctgataata aaaatggaat aatcattaat aagagaaaac
    12361 agaaaccacc gttatgggtt agcgcactaa ataaccgcca tcgaccacta ataaatgccc
    12421 gttgacataa ttcgatgcct gacttgcgag aaacacggct gcgcccatta aatcctgagt
    12481 atcgccccaa cggtttgccg gaatatgatc aagaacgcgc tgattggttt ctggattact
    12541 gcgtgtcgcc agcgtaatat ctgttgcata atagccaggg gcgataccat ttacctgaat
    12601 attatattga cctagttcat cacaataagc tttggtgaat ccggcaagag catgtttagt
    12661 agcagaatat gcaggtgacc attgtccacc taagtaagag aacaatgaac agatattaat
    12721 gattttaccg cttttctgcg ggatcataat ttttgccgct tcgtagctta actcgaatgc
    12781 ggcggtcagg ttcacatcaa tcattggatc ccagtcggca cgaccgaagt ccagcacctt
    12841 attcagctta caaataccgg cattgttaac cagaatatca actgtaccga aacgctcaca
    12901 gcaggcagcg ataatcttct gcggcgcgcc ttctgcggtg atatccacct gcatgaagtc
    12961 cacctcaaca ccctgatttt caatcatttc ctttgtttca ccgttatctt tgacaaaact
    13021 tggaataaag acatttgcgc ctgctttggc caacgccatg gcaaatgcct ggcctaaacc
    13081 gctattcccg ccggtaacaa ttgcggtttt acctttcagg gagaaaaaat ccattgagaa
    13141 cgcattgaga gattcgattg acatagttag ctccaatttc cgtaaggcaa aaaaaaagaa
    13201 aggtaacttc ccccgcgaga gggaagttac ctttctcatt atctctggta actggagata
    13261 atttttagca taaggacata actgctaatg tgatcgtaat cacagtgtga tattcgcatt
    13321 atcaaatagt tttaaaaagc agaagcgtga ttactctcac acaacacctc tgctttccat
    13381 gttatttctt attgcaatta ctagagacct gagaaaagta atccacctga aagggcggct
    13441 tacttttctc ttttttttgt ctattacaaa tgggaatacg atatgcaaca caactcatat
    13501 cgccgttgga taaccctcgc gataattagt tttagcggcg gcgttagttt tgacctggct
    13561 tatttacgtt atatttatca aattcccatg gcgaaattta tgggattcag caataccgag
    13621 ataggtttaa taatgagtac ctttggtatt gcagctatta ttctttatgc ccccagcggc
    13681 gttattgccg ataaattctc acatcgcaaa atgattactt ccgcgatgat cattaccgga
    13741 ttactgggtc tgttaatggc aacgtatcca ccgctgtggg taatgctctg tattcaggtc
    13801 gcctttgcga taacgacgat tttaatgctg tggtcggtgt cgattaaagc cgcctcattg
    13861 ctgggcgatc atagtgagca agggaaaatt atgggctgga tggaagggct gcgcggcgtc
    13921 ggtgtaatgt cgctggcggt gtttaccatg tgggtctttt ctcgctttgc accggatgac
    13981 agcgccagcc tgaaaacggt cattatcatc tatagtgtgg tttacatctt gttggggatt
    14041 ctgtgctggt tttttgttag cgataacaac aacctgcgca gtgccaataa cgaagaaaaa
    14101 caatcattcc agcttagcga catcctggcc gttttgcgca tcagcaccac ctggtattgc
    14161 agcatggtga tttttggtgt cttcaccatc tacgccattc tgagttactc caccaactat
    14221 ctgaccgaaa tgtatggcat gtcgctggtg gcggcgagct acatggggat tgtgatcaac
    14281 aaaatcttcc gcgcgctgtg cggcccactt ggcggcatta tcaccaccta cagtaaagtg
    14341 aaatccccta cccgcgtgct ccaaatcctt tccgtactcg gcctgctggc gttaactgcc
    14401 ctgctcgtca cgaactctaa cccgcaatcg gtcgcgatgg ggattggcct gattttactg
    14461 ctgggattca cctgttacgc ctcacgcggg ctgtattggg cctgccctgg cgaagcaaga
    14521 acaccgtctt acattatggg caccacggta ggtatttgtt cggtgatagg attcctgccg
    14581 gatgtcttcg tttacccaat tatcggccac tggcaggaca ccctgcccgc agcagaagcc
    14641 taccgcaata tgtggctgat gggcatggcg gcgctcggca tggtgatcgt ctttaccttt
    14701 ttgttgttcc aaaaaattcg tactgctgat atcgccccca caacggctaa cagcaagtaa
    14761 gccagaaaaa ttacaggaga aaaatcatgt cgaagaaata catcataggg attgatggcg
    14821 gaagtcagag cacaaaagtg gtgatgtacg atctggaagg taacgtggtt tgcgaaggca
    14881 aaggtttatt gcaaccgatg cacacgccag atgccgatac cgcagaacat cctgacgacg
    14941 atttatgggc atcattatgt tttgccggtc acgatttgat gagtcagttt gccgggaata
    15001 aagaagatat tgtcggtatt ggtctgggat ccatccgttg ctgccgtgcg ttattgaaag
    15061 ccgatggcac gccagctgcg ccattgatta gctggcagga tgcacgcgta acgcgtccct
    15121 acgaacacac taatcctgac gtggcatatg tcacctcttt ttcgggttat ctgatgcatc
    15181 gcttaaccgg cgagtttaaa gacaatatcg ccaactattt tggtcagtgg ccggtggatt
    15241 ataagacctg ggcatggagt gaagatgctg cggtaatgga taagtttaat atcccccgtc
    15301 atatgctgtt tgatgtgcaa atgcctggca ccgtcctcgg acatatcaca ccacaagccg
    15361 cactggcgac acatttcccg gcaggactgc cggttgtttg taccaccagt gataaaccgg
    15421 tagaagctct gggggccgga ttactggatg atgaaactgc ggtaatttct cttggcactt
    15481 acatcgcatt gatgatgaac ggcaaagcac tgccgaaaga tccggtggcg tactggccga
    15541 ttatgtcttc tattccgcaa acattgctgt atgaaggtta cggtattcgc aaaggcatgt
    15601 ggacggtgag ctggctgcgc gacatgctag gcgagtcgtt aattcaggat gccagggcgc
    15661 aggatctttc accggaagat ttactcaaca aaaaagcttc ttgtgtgccg ccaggctgta
    15721 atggtctgat gacggtgctg gactggctga ccaatccgtg ggaaccgtac aaacgcggga
    15781 ttatgatcgg ctttgattcc agcatggatt acgcatggat atatcgttcg atactggaaa
    15841 gcgtggcgct gacgctgaag aacaattacg acaatatgtg taatgaaatg aatcactttg
    15901 cgaagcatgt aatcattact ggcggcggtt cgaacagcga tctgtttatg cagatttttg
    15961 ccgacgtgtt caaccttccg gcacgacgta acgccattaa cggttgtgca agtctggggg
    16021 cagcgattaa tacagcggta ggtctggggc tatacccgga ttacgcaacg gctgtcgata
    16081 aaatggttcg cgtgaaagat atctttatgc ctgttgagag caatgccaaa cgctacaacg
    16141 cgatgaataa aggcattttc aaagatttaa ccaaacacac tgatgtgatt ctgaaaaaat
    16201 cgtatgaagt gatgcatggg gaattgggga atgcggattc gatccagagc tggtcgaatg
    16261 cgtaagtgag gaagaccggg cgggaaactg cccggcctga acatacctga atggttatcc
    16321 ccgctggcgc ggggaacata agttcgacaa cttaaaaaat gataacaagt ccggtttatc
    16381 cccgctgatg cagggaacat aataaaaact ttaactagtt cggtttatcc ctgtttttac
    16441 aaggcatatt aactacactc gatgtcttta gaataaaaga caatacg
//
LOCUS       DAZKFU020000093        16449 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZKFU020000093 DAZKFU020000000
VERSION     DAZKFU020000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.094"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(377..753)
                     /gene="rnpB"
                     /locus_tag="ACHOQQ_RS12155"
                     /old_locus_tag="pgaptmp_002431"
     ncRNA           complement(377..753)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHOQQ_RS12155"
                     /old_locus_tag="pgaptmp_002431"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(786..1931)
                     /gene="garK"
                     /locus_tag="ACHOQQ_RS12160"
                     /old_locus_tag="pgaptmp_002432"
     CDS             complement(786..1931)
                     /gene="garK"
                     /locus_tag="ACHOQQ_RS12160"
                     /old_locus_tag="pgaptmp_002432"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGU4470671.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2028..2918)
                     /gene="garR"
                     /locus_tag="ACHOQQ_RS12165"
                     /old_locus_tag="pgaptmp_002433"
     CDS             complement(2028..2918)
                     /gene="garR"
                     /locus_tag="ACHOQQ_RS12165"
                     /old_locus_tag="pgaptmp_002433"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4470672.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVVLGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2948..3718)
                     /gene="garL"
                     /locus_tag="ACHOQQ_RS12170"
                     /old_locus_tag="pgaptmp_002434"
     CDS             complement(2948..3718)
                     /gene="garL"
                     /locus_tag="ACHOQQ_RS12170"
                     /old_locus_tag="pgaptmp_002434"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGU4470673.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3734..5068)
                     /gene="garP"
                     /locus_tag="ACHOQQ_RS12175"
                     /old_locus_tag="pgaptmp_002435"
     CDS             complement(3734..5068)
                     /gene="garP"
                     /locus_tag="ACHOQQ_RS12175"
                     /old_locus_tag="pgaptmp_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGU4470674.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSGEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5443..7014
                     /gene="garD"
                     /locus_tag="ACHOQQ_RS12180"
                     /old_locus_tag="pgaptmp_002436"
     CDS             5443..7014
                     /gene="garD"
                     /locus_tag="ACHOQQ_RS12180"
                     /old_locus_tag="pgaptmp_002436"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGU4470675.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7163..7498
                     /gene="prlF"
                     /locus_tag="ACHOQQ_RS12185"
                     /old_locus_tag="pgaptmp_002437"
     CDS             7163..7498
                     /gene="prlF"
                     /locus_tag="ACHOQQ_RS12185"
                     /old_locus_tag="pgaptmp_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGU4470676.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7498..7962
                     /gene="yhaV"
                     /locus_tag="ACHOQQ_RS12190"
                     /old_locus_tag="pgaptmp_002438"
     CDS             7498..7962
                     /gene="yhaV"
                     /locus_tag="ACHOQQ_RS12190"
                     /old_locus_tag="pgaptmp_002438"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGU4470677.1"
                     /translation="MDFPQRVNGWALYTHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8017..8826)
                     /gene="agaR"
                     /locus_tag="ACHOQQ_RS12195"
                     /old_locus_tag="pgaptmp_002439"
     CDS             complement(8017..8826)
                     /gene="agaR"
                     /locus_tag="ACHOQQ_RS12195"
                     /old_locus_tag="pgaptmp_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGU4470678.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRLQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9075..10355
                     /gene="kbaZ"
                     /locus_tag="ACHOQQ_RS12200"
                     /old_locus_tag="pgaptmp_002440"
     CDS             9075..10355
                     /gene="kbaZ"
                     /locus_tag="ACHOQQ_RS12200"
                     /old_locus_tag="pgaptmp_002440"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGU4470679.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPV
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10378..10851
                     /gene="agaV"
                     /locus_tag="ACHOQQ_RS12205"
                     /old_locus_tag="pgaptmp_002441"
     CDS             10378..10851
                     /gene="agaV"
                     /locus_tag="ACHOQQ_RS12205"
                     /old_locus_tag="pgaptmp_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGU4470680.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10862..11641
                     /gene="agaW"
                     /locus_tag="ACHOQQ_RS12210"
                     /old_locus_tag="pgaptmp_002442"
     CDS             10862..11641
                     /gene="agaW"
                     /locus_tag="ACHOQQ_RS12210"
                     /old_locus_tag="pgaptmp_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGU4470681.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11631..12509
                     /gene="agaE"
                     /locus_tag="ACHOQQ_RS12215"
                     /old_locus_tag="pgaptmp_002443"
     CDS             11631..12509
                     /gene="agaE"
                     /locus_tag="ACHOQQ_RS12215"
                     /old_locus_tag="pgaptmp_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGU4470682.1"
                     /translation="MASNQTTLPNISENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTMTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12527..12961
                     /gene="agaF"
                     /locus_tag="ACHOQQ_RS12220"
                     /old_locus_tag="pgaptmp_002444"
     CDS             12527..12961
                     /gene="agaF"
                     /locus_tag="ACHOQQ_RS12220"
                     /old_locus_tag="pgaptmp_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGU4470683.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12958..14091
                     /gene="nagA"
                     /locus_tag="ACHOQQ_RS12225"
                     /old_locus_tag="pgaptmp_002445"
     CDS             12958..14091
                     /gene="nagA"
                     /locus_tag="ACHOQQ_RS12225"
                     /old_locus_tag="pgaptmp_002445"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4470684.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRYLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQGQLASF"
     gene            14442..15596
                     /locus_tag="ACHOQQ_RS12230"
                     /old_locus_tag="pgaptmp_002446"
     CDS             14442..15596
                     /locus_tag="ACHOQQ_RS12230"
                     /old_locus_tag="pgaptmp_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGU4470685.1"
                     /translation="MPENYTPAAAASGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15609..>16449
                     /gene="kbaY"
                     /locus_tag="ACHOQQ_RS12235"
                     /old_locus_tag="pgaptmp_002447"
     CDS             15609..>16449
                     /gene="kbaY"
                     /locus_tag="ACHOQQ_RS12235"
                     /old_locus_tag="pgaptmp_002447"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGU4470686.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGS"
BASE COUNT         3948 a         4380 c         4224 g         3897 t
ORIGIN      
        1 acaaagtcta ttattaatac cgtagatatt catcttaccc tgtgcaccaa aagcaaaaac
       61 ttatcgacgc gggtttgaaa ctgatggcaa ccgcaaaaat gcctgatgcg ctacgcttat
      121 caggcctacg ccatctctgc aatatattga atttgcgtgc ttttgtaggc aggataaggc
      181 gttcacgccg catccggcat gaataaagcg cactcaacag tctgaaaccc gccggaacgg
      241 tttattacgt acatcaggtg aaactgaccg ataagccgct ttcttttggg tctagcgtcg
      301 tggacagtca ttcatctttc cgcccctcca aaagcaaaaa cccgccagtt tacgctggcg
      361 ggtttttacg taaatcaggt gaaactgacc gataagccgg gttctgtcgt ggacagtcat
      421 tcatctaggc cagcaatcgc tcactggctc aagcagccta cccgggttca gtacgggccg
      481 taccttatga acccctattt ggccttgctc cgggtggagt ttaccgtgcc acggactgtt
      541 accagccgcg cggtgcgctc ttaccgcacc ctttcaccct tacctgatcc cgcttgcgcg
      601 ggccatcggc ggtttgctct ctgttgcact ggtcgtgggt ttccccccca ggcgttacct
      661 ggcaccctgc cctatggagc ccggactttc ctcccctccg cccgtctccc ccgaagagga
      721 cgacgacgaa gcggcgactg tctggtcagc ttcggcgcgc agtatagagg gtttgcgcgc
      781 ccttgtcacc ccgcgttgcg cattccaatc gccagtgtcg cggcgatatt acgtgaagca
      841 cggcagatat tgtcataagc cccgcggaat gcttcgtcca acgtacctat gctggtcaat
      901 acgctgaaga ccgcatcaat gccatgctga tgtacaacgc caacatcatc ggtcaggcta
      961 cccgcaatgc caatcaccgg tttatggtac ttcttcgcca cgtttgcgac accaatcggt
     1021 accttgccgt gaatactctg gctgtcaata cgcccttcac cggtgatcac cagcgtacaa
     1081 tcgtgaatat gttcctccag attcagcgcc gtagtgacga tttcaatacc acttttcagt
     1141 tccgcaccaa gaaacgccat tagcgccgcg cccataccac ccgcagctcc tgcgccgggg
     1201 acatctttca catcaacatg cagcgctttt ttaatgacct cggcatagtg agagaggtta
     1261 ttgtccagct caacaatcat cgcttcactg gctccctttt gtgggccaaa gatgcgcgat
     1321 gcgccgttat cgcccaccag cggattggtg acatcacaag cgacgcgaat gacgcaatct
     1381 tttaagcgcg gatcgaggcc ggaaatatca atatcattca gagtattaag actaccgccg
     1441 ccaaaaccaa tttcattgcc gttggcgtcg cataatttcg cccccagcgc ctgtaccatg
     1501 cctgcgccgc catcatttgt agcgctgccg ccaatgccga taataatgtt tgtcgcgccg
     1561 ctctccagcg cctgcaggat taactcgcct gtgccgcgtg aagtggtcac gagtggatcg
     1621 cgtttttccg caggtaccag ctccagccca ctggccgccg ccatttcaat aaacgcggtt
     1681 ttgccatcgc cggagatccc ccaactggca ttcactttct cgcccagcgg ccctgtaacc
     1741 caggcgtgac gttcggaacc ctgggtggct gcaatcatcg cttccaccgt tccttcgcca
     1801 ccgtcggcaa caggaacaga aacgtactgt gcatcaggaa aaatttcccg aaatcctttt
     1861 tctatcgcct gcgcaacctc gctggcagat aaactttctt tataagagtc tggggcgatt
     1921 acgattttca tacctatgcc tgttaccaca tgacgccgga gggcgtttct cttattcggc
     1981 ctggattcca ggcccggatt gcaatacgcc atccgggcac gacgtcatta acgagtaact
     2041 tcgactttcg ccagtttttc gtagtagcac gccagggcgc tatgatccgc cgttcctaaa
     2101 ccatctgcac gcagtgcctg catcatctcc ataaccgcag ctgtgagcgg cagttgcgcg
     2161 ccgacaccgt gagaagtatc cagcgcattc gccagatcct taatatgcag atcaatacgg
     2221 aagcccggct tgaagttgcg gtccatcacc atcggcgctt tggcatccag cacggtactg
     2281 cccgccagcc caccacgaat tgcctgataa accaggtccg ggttaacgcc cgctttagtt
     2341 gccagcgtta acgcttctga catcgcggca atattcagcg ccacaatgac ctgatttgcc
     2401 agtttagtga cgttacctgc accgatttcc ccggtatgca ccacggaacc cgccatcgct
     2461 ttcatcaaat catagtattt gtcgaaaata gccttgtcgc cgcccaccat cactgacagc
     2521 gtaccgtcga tggctttcgg ttcaccgccg ctcaccggag catccagcat atcaatgcct
     2581 ttcgctttca gcgcttcgct gatttcacgg cttgccagcg gtgcgataga actcatatcg
     2641 atcaataccg tacctggctt cgcgccttca ataatgccat tctcacccag caccacctct
     2701 ttcacatgag gggagtttgg cagcatggtt atgatgacgt cgcactgttc agcgatcgct
     2761 ttagccgtag aagctgtttc tgcacctgca gcaatcacgt cagcaatagc ttctgggtta
     2821 cggtcagcaa ccaccagcga gtaacctgct ttcagaaggt ttttactcat tggtttaccc
     2881 ataatcccca ggccaataaa accaactttc atagtcatat caatcatctc tcttgttgcg
     2941 gtggtggtta ttttttaaag gtatcagcca gtttctgagt ggcagagcgg aagacgccga
     3001 gatcgctgcc gacagccaca aacgtcgcgc cccattccag ataacgacgc gcatcggctt
     3061 cgaccggcgc gaggataccg ctgggtttgc cgtgcgcgct ggcacggtta aaaatgtgct
     3121 gaattgcttt ttgtacatcc gggtgtgatg cattgccgag atggcctaat gccgcggcca
     3181 gatcgctggg gccgacgaag atgccgtcta cgccttcggt agcggcaata gcatcgacgt
     3241 tatctacgcc ctgctgactt tctatctgga ccagaatagt gatgttcttg ttcgactgag
     3301 cgaaataatc cgccacggtg ccaaacatat tggcgcggtg agaaacggag acgccgcgaa
     3361 tgccttctgg cggataacgc gtcgatgcca ccgcctgctc tgcttcctct tttgtttcta
     3421 caaaaggaat caggaagtta tagaaaccga tatccagaag acgcttaata attaccggct
     3481 cgttggtcgg cactcgtact actggcgcgc tggcgctgcc tttcagagcc attaactgcg
     3541 gaataaacgt ggagatatcg tttggcgcat gttcgccatc cagcaccagc cagtcaaacc
     3601 cagccaaacc aagaacttca gtgctaatcg ggttagagag tgctgaccag caaccaattt
     3661 gtacctgttt cgcagccaat gcggctttga atttattcgg gaaaacatcg ttattcatcg
     3721 cttatacctt tgcttatttc tgcaattcca tacgtttaat gtcgccaact acgaagaggt
     3781 agcagaccat cgccatcagc gctgaacatc ccacgaaaac cagtgctgca ttgaaggagt
     3841 gcagttcact taccaggtag ccaatcacca gtggagtgac aatggaggca acattgccaa
     3901 agacgttaaa gacgccgccg cagaggccaa caatctcttt cggcgcggtg tcagaaatca
     3961 ccggccagcc cagcgcacca aatcctttgc caaagaaagc cagcgccatc agcatgacca
     4021 ccagcgtggt gttgttggtg tagttacata agatgatggt ggaagccagc aacattccca
     4081 gcacaatcgg tagcttacgt gccagggtca gggataaacc gcgtttgatc agataatccg
     4141 agaagacacc tcccagcacg ccgcccgcaa aaccacacag tgctggaatc gaggcgacca
     4201 gacccacttt cagaatcgac atgccttttt cctgcaccag ataaatcggg aaccaggtga
     4261 ggaagaacca ggtgatggtg ttgataaaat attgtccgaa aaatacgccc aacatcatgc
     4321 ggttagagag caattgcttg atgtaatgca gtttgggacc gcttgctgcc gcgctgcccg
     4381 gctttttgtg gtccatatcg accaccgcgc cattttcaga gataaacttc agttcttccc
     4441 cagacatacg tgggtgatct gtcgggttat gaatcaattt gatccacagc gccgtcagca
     4501 caaaaccaat cacccccata acggtaaaga cgtgctccca gccccaggcg aaagtcagcc
     4561 agccaagcag aggcgaaaag agcgccagcg agaaatattg cgccgagtta aagatggcgg
     4621 aggcagtacc acgttctttc gtcgggaacc aggcggcgac aatacgggcg ttcgccggga
     4681 atgatggcgc ttccgagaag ccgagcataa agcgcataaa gaacatggag atccctgccc
     4741 aggccagcgg gaacatatca acaaagcctt gcaggaaggt gaatagcgac cagaaaaaga
     4801 ggctgtaggt gtaaactttt ttcgagccaa acttatcaag cagccagccg ccggggattt
     4861 gcatcagcaa gtaggcccag ccaaaagcag agaagatgta acccatcgaa accgcactta
     4921 actgcaactc ttttgccact tcggtaccag caatagacag cgttgcacga tcggcgtagt
     4981 taacggcggt aacaataaaa ataatcagta atattaaata gcgggtatgc acgcctttct
     5041 ttttttcgtc aacggtgtcc agaatcattt tatttacctc gggtacttat gctgattttt
     5101 attattatgg ggaaggtgtt atttatgagt ttcatttatg ccgtaacgac aatgaactcg
     5161 ggaattagta taagcagcgc gagaataata atcattgtgc aaatgctaat ttaattaata
     5221 ctatttaaat attattttga gcatatgcac ataaggttgc gcgctaaagc acagatttgc
     5281 gctttacctt gccgggcggc attgcaatcc ctgaaatgat tgacattgat cacatttctg
     5341 catttaaact cctgacattc ttatttcacc caatgaagtc atttattttt aaatgagacc
     5401 aggtcctcat tttaataacc cctggctgga gaatattgca caatggccaa catcgaaatc
     5461 agacaagaaa cgccaactgc gttttatata aaagttcacg acacagataa tgtggcaatt
     5521 attgttaatg ataatggcct gaaagcggga acgcgttttc cggatgggct ggaattaatt
     5581 gaacatattc cccaggggca taaagtcgca ttgctggaca ttccggctaa tggtgaaatt
     5641 attcgttatg gcgaagtgat tggttacgcc gtgcgtgcaa tcccacgcgg aagctggatc
     5701 gacgaatcaa tggttgtact accggaagcg ccgccgttac acacgctgcc actggcaacc
     5761 aaagtcccgg aacccttacc gccgctggaa ggatacacct ttgagggcta tcgcaatgcc
     5821 gatggcagcg tgggcaccaa aaacctgctc ggcatcacca ccagcgtcca ctgtgtggca
     5881 ggcgtggtgg actacgtagt aaaaatcatt gaacgcgatc tgctgccgaa atacccgaac
     5941 gtcgatggcg tggtggggct gaatcatctg tacggttgtg gcgtggcgat taacgctccg
     6001 gcggcagttg tacctatccg taccattcac aatatttcgc tgaatcctaa ctttggcggc
     6061 gaagtaatgg tgattggcct gggttgtgaa aagttgcagc ctgagcgcct gctgactgga
     6121 acggatgatg tgcaagctat tccagtagaa agcgccagca ttgtcagttt gcaggatgaa
     6181 aagcatgtcg gttttcagtc catggtcgag gatattttgc aggtcgccga acgccattta
     6241 caaaaactga atcaacgtca gcgagaaacc tgtccggctt cagaactggt tgtcggcatg
     6301 cagtgcggtg gcagcgatgc cttttctggc gtaacggcaa acccggcggt tgggtatgcg
     6361 tctgatctac tggtgcgctg cggcgcaacg gtgatgttct cagaagtcac ggaagtgcgt
     6421 gacgcgatcc atctgctgac accacgcgca gtgaacgaag aggtcggcaa acggctgctg
     6481 gaggagatgg agtggtacga taactatctc aatatgggaa aaaccgaccg cagcgccaac
     6541 ccttcgccgg gcaacaagaa aggcggtctg gcaaatgtag tggagaaagc gctcggctcc
     6601 attgctaaat cgggtaaaag tgcaattgtt gaagtgctgt cgcccggtca acgcccgact
     6661 aaacgcggat taatttacgc cgcgacgcca gccagcgatt ttgtctgtgg cacgcaacag
     6721 gtggcttcgg gtatcaccgt gcaagtgttt acgaccggtc gtggtacgcc gtacggcctg
     6781 atggcggtac ccgtcattaa aatggcgacc cgcaccgagc tggcgaaccg ctggtttgat
     6841 ttaatggata ttaacgcggg cactatcgcc accggcgaag aaaccattga agaggtgggc
     6901 tggaagttgt tccactttat tctcgacgtc gccagcggga agaagaaaac cttctcggat
     6961 caatggggac tgcataacca gctggcggtg tttaacccag caccggtgac ctgatttctt
     7021 ttggaacgtc cctcgcaaaa catggcctta gtgccatgtt tttattgttt aaagccccca
     7081 cgtccattaa taatgcattt gcattacctt agttcaagct tataatttga gcagaaaaca
     7141 ggctgtaaaa ggacagtgaa tcatgcccgc taatgctcgc tctcacgctg tactgaccac
     7201 tgaatcaaag gtcacgatac gcggacaaac aactatcccc gcgccagtgc gtgaggcctt
     7261 aaaactgaag ccaggccagg acagcattca ttacgaaatt ctgcctggtg ggcaagtatt
     7321 tatgtgccga ctgggagatg aacaggagga tcatactatg aatgcatttt tgcgttttct
     7381 ggatgcagat atccagaaca acccgcaaaa aactcgtcca ttcaacattc aacaaggaaa
     7441 gaaacttgtc gctggcatgg acgtcaacat tgatgatgag attggcgacg acgaataatg
     7501 gattttccac aaagggttaa tggttgggcg ctatatactc atccctgttt tcaggaaacc
     7561 tacgacgctt tagttgccga agtcgagaca ttaaagggaa aagatcctga aaattatcag
     7621 agaaaagccg ccacaaagtt attggcggta gtccataaag tgattgagga gcatatcacg
     7681 gtcaatccat catcaccggc attccgtcat ggcaagtcgt taggctctgg gaaaaataaa
     7741 gactggtcac gggtaaaatt tggtgctggt cgttatcgtc tcttctttcg ttatagtgaa
     7801 aaagagaaag tcatcattct gggatggatg aacgatgaaa acacgctgcg cacctacggt
     7861 aaaaaaacag atgcctatac cgtattcagc aaaatgttaa aaagaggaca tcctcctgcc
     7921 gactgggaaa ccctcacccg agagacagaa gaaacccatt gatggtgttc acaatgcccg
     7981 ccgaacgcat tcgtgttgac cagcgggcac cctttctcac tccccgacca gaatcacttc
     8041 aaccccagcc tttcgcagtc cttccagact atccgcagga atgccttcat caacaatgat
     8101 catgtcgata cgttgagtat caatgatctt atgtaaactg gagcggttga acttactgga
     8161 atcggtgact acgatgatcc gttccgcaac ttcgcacatc cggcggttta aacgggcttc
     8221 atcttcatta tgcgtgctga cgccgcgctc cagatcaatc gcatctacac caagaaacag
     8281 catatcgaag tggtaatttt gcagcgattg ctcagcctga tcgccgtaaa aagattgcga
     8341 ctgacggcgc aaatgcccgc cggtcatcag cagctcaacg ccttccgctt ccagcaaagc
     8401 attagccacg ttcataccgt tggtcatcgc aattacgtca gtgtgcttgc gcatcagacg
     8461 agcaatctca aaagtggtgg tcccggaatc gaggatcacc cgatgacctg gctgaatcaa
     8521 ctcaacggca gctttcgcaa cgctgcgttt cattgcggtg ttcagcgcgc ttttatcttc
     8581 cactgatggc tcgactgacg gcgttgtgct atcgcagatc aacgcgccac cataggcacg
     8641 cacagcgatc ccctgctttt ccagaaacgc cagatcgttg cggatcgtca cagtagatac
     8701 gccatacaat gccgacagat cgttaacctg cacactccct tgcagtcgca gacgctgaat
     8761 gatctgttct cgtcgctcgc tggtgcctgt cactcgcttc tcacctgaag cgtcggtatt
     8821 actcatagta agtcctttcg taaaactttc gtttcatttc gttttgccta ttaacgcctt
     8881 tctattaagc aaatgcaagc ccaccttgcc cattgacgca agctactctc gtttcagtga
     8941 ctttcattat gtttcttttg tgaatcagat cagaaaacca ttatctttcg ttttattttt
     9001 atctcaccat gacgcagtat caactgaaac aaaacgaaag attaatatcg cagtaatctg
     9061 aactggagag gaaagtgaaa catctgacag aaatggtgag acagcacaaa gcgggcaaaa
     9121 caaatggaat ttatgccgtt tgttccgcac atccgctggt gctggaggct gcaatccgct
     9181 acgccagtgc aaaccaaacg ccgttactga ttgaagcaac ctccaatcag gtagaccagt
     9241 tcggcggtta taccggaatg acgcccgccg attttcgcgg ctttgtttgt cagctcgccg
     9301 actcgttgaa tttcccgcag gatgcgttga ttctgggtgg tgaccatctg gggccaaacc
     9361 gctggcaaaa cctgccagtc gctcaggcaa tggccaatgc cgatgatttg attaaaagct
     9421 acgttgcggc aggattcaaa aaaatccacc ttgattgcag catgtcctgt caggacgatc
     9481 caattccctt aactgatgac atcgtggctg aacgcgccgc ccgtctggcg aaagtggcgg
     9541 aagaaacctg tcttgaacac tttggcgaag ccgatctgga gtatgtcatt ggtaccgaag
     9601 tgccggtacc tggcggcgcg catgaaacct taagcgagct ggcggtcacc acgccggatg
     9661 ccgcccgcgc cacgctggaa gcccatcgtc acgcctttga aaagcaaggt ttgaatgcca
     9721 tctggccacg catcattgcc ctggtggttc aacccggcgt cgaattcgat cacaccaacg
     9781 ttattgatta tcagcccgcc aaagcgaccg ccttaagcca gatggtcgaa aactacgaaa
     9841 cgctgatttt cgaagcgcac tctaccgatt atcaaacgcc gcaatcgctg cgccagctgg
     9901 tgattgacca ctttgccatt ctgaaagttg gcccagcgct gaccttcgcc ctgcgtgaag
     9961 ctctgttctc tctggcagcg attgaagaag aactggtgcc agcaaaagcc tgttctggtc
    10021 tgcgtcaggt gctggaaaac gtgatgctcg accgcccgga atactggcaa agccactacc
    10081 acggtgacgg caacgcgcgt cgtctggcgc gtggttatag ctactcggat cgcgtgcgct
    10141 attactggcc ggacagccag attgatgacg ctttcgctca tctggttcgt aatctggcgg
    10201 attcaccaat tccgctgccg ctgatcagcc agtatctgcc gctgcagtac gtgaaagttc
    10261 gctccggcga gctgcagcca acgccacggg aactcattat caaccatatt caggacatcc
    10321 tggcgcagta ccacacagcc tgtgaaggcc aataagcaaa acaaagagga acacgctatg
    10381 ccaaatattg ttttaagccg gattgatgaa cgcttgattc acggtcaggt cggcgttcag
    10441 tgggtcggat ttgcgggggc aaatctggtg ctggtcgcca acgatgaggt tgccgaagat
    10501 ccggtacaac aaaacctgat ggaaatggta ctggcagaag ggatcgccgt acgtttctgg
    10561 acgctgcaaa aagttatcga caacattcat cgcgccgccg atcgacagaa aatcctgctg
    10621 gtttgtaaaa cacccgccga tttcctgacg ctggtgaaag gtggcgttcc ggtgaatcgc
    10681 attaacgttg gcaatatgca ctacgccaat ggcaagcaac aaatcgccaa aacggtttct
    10741 gtggatgcgg gcgatatcgc agcatttaac gacctgaaag ccgctggggt ggaatgcttc
    10801 gttcagggcg tcccgacaga gcctgctgtg gacctcttta aattactttg agggattcat
    10861 catggaaatc agtctgttgc aggcattcgc gttgggcatt atcgccttta tcgctggcct
    10921 ggatatgttt aacggcttaa cccatatgca ccgcccggtg gtcctcggcc cgttagtcgg
    10981 gctggtactt ggcgatctgc ataccggtat tttaaccggc ggtacgctgg aactggtgtg
    11041 gatggggctg gccccgctgg cgggcgcaca gccgcctaac gtgattatcg gtactatcgt
    11101 cggcacggcg tttgccatta ctactggcgt gaaacccgat gtcgcagtcg gtgtcgccgt
    11161 acctttcgct gtcgcagtac agatggggat taccttcctg ttctcggtga tgtctggcgt
    11221 gatgtctcgc tgcgaccgga tggcggagaa cgccgatact cgcggcattg aacgcgtaaa
    11281 ctatctggca ctgctggcac tcggcacttt ctattttctc tgcgctttcc tgcctatcta
    11341 cttcggcgcg gaacatgcaa aaaccatcat tgatgtccta ccgcaacgat taatcgacgg
    11401 cctcggcgtc gcgggcggca tcatgccagc aatcggtttt gccgtgctgc tgaaaatcat
    11461 gatgaaaaac gtctacatcc cctacttcat cctgggcttt gttgccgcag cctggctcaa
    11521 gttaccggtg ctggctattg ctgccgccgc gctggcgatg gcgctgatcg atctgctgcg
    11581 taaatctcct gaaccgactc aacctgcggc acagaaagag gaatttgaag atggcatcta
    11641 atcaaaccac cctgccgaac atcagtgaaa acgaagaaac attgctgact ggcgtcaatg
    11701 aaaacgtgta tgaagatcag agcattggcg cggagctgac gaaaaaagat atcaatcgcg
    11761 tcgcctggcg ttccatgctg ttacaagcgt cttttaacta cgaacgtatg caggcttccg
    11821 gctggttgta cggtctgctg cctgctctga aaaagatcca cactaataaa cgcgacctgg
    11881 cgcgcgccat gaaggggcat atgggtttct tcaataccca tccgtttctg gtgacatttg
    11941 ttatcggcat tatccttgcg atggagcgtt ctaagcagga cgttaacagt attcagagca
    12001 ccaaaattgc cgtcggtgcg ccgctcggcg ggattggcga tgcaatgttc tggctaacgc
    12061 tactgccgat ttgtggcggg attggagcca gtctggcatt gcaaggctcc attcttggcg
    12121 cagtcgtctt tattgtgctg ttcaacgtgg tacacctggg gctgcgtttt ggtctggcgc
    12181 attatgctta ccgcatgggc gtggcggcga ttccactcat taaagcaaat accaaaaaag
    12241 tcggccatgc tgcgtctatc gttgggatga cggtaatcgg tgcgctggtg gcaacctatg
    12301 ttcgtttaag caccacgctg gaaatcacag cgggcgacgc agtggttaag ttacaggctg
    12361 atgttatcga caaactgatg cctgccttct taccgttggt ctacaccatg accatgtttt
    12421 ggttggtacg ccgcggctgg agtccgctgc gtctgattgc agttaccgtg gttctcggca
    12481 tcgtcggtaa attctgccat ttcctttaaa tacaatgagg tattagatgt taagtattat
    12541 tttgacaggg catggcgggt ttgccagcgg catggaaaaa gcaatgaagc aaattctcgg
    12601 tgagcaatcg cagtttatcg ccatcgattt tccggaaacc tcaagcaccg cgctgcttac
    12661 atcgcagctt gaagaggcca tcgcccaact ggattgtgaa gacggcattg tttttctgac
    12721 ggatttgctg ggcggcacgc cgtttcgcgt agcttcaaca ctggcgatgc aaaaaccggg
    12781 ctgtgaagtg atcaccggca ccaatttgca actgctactg gagatggtgc tggagcgcga
    12841 agggttaagc ggtgaagaat tccgtgtgca ggcgctggag tgtggacatc gtgggctgac
    12901 cagtctggtg gacgagttag gccgctgcca tgaggaatgt ccggtcgagg aaggaatatg
    12961 acacacgttc tgcgcgccag aaggctgctg actgaagagg gatggctcga tgaccatcag
    13021 ttgcgtattg ctgacggtgt catcgcagca atcgaaccga ttccagtggg cgtgactgaa
    13081 cgcgatgcgg aactgctctg ccctgcttac atcgacaccc atgtacacgg tggtgcgggc
    13141 gttgatgtaa tggatgacgc gccggatgta ctcgacaagc tggcaatgca caaggcacgc
    13201 gaaggtgtcg gcagttggtt accgaccacc gtaaccgcgc cgcttaatac cattcatgcg
    13261 gcgctgaaac gtattgctca acgttgccaa cgcggcggac ctggtgcgca agtgctgggg
    13321 agttatctcg aaggaccgta cttcacgccg cagaataaag gcgcgcatcc gccggaattg
    13381 tttcgcgagc ttgaaattgc cgagctggat caattgattg ccgtttctca gcacacctta
    13441 cgcgtggtag cgctggcacc ggaaaaagaa ggggcattgc aggccatccg ctatcttaaa
    13501 cagcaaaatg taagggtgat gctggggcat agcgcggcga cctggcaaca aacccgcgcc
    13561 gcgtttgatg ccggtgccga cggcctggtg cattgctata acgggatgac aggtttacat
    13621 caccgcgaac cgggaatggt tggcgcggga ttaacggaca agcgcgcctg gctggaactg
    13681 atagccgatg gtcatcatgt gcatccggcg gcaatgtcgc tgtgttgttg ctgtgcgaaa
    13741 gagagaatcg tgctgatcac cgacgcgatg caggcagccg ggatgccgga tggtcgctat
    13801 acgttatgtg gtgaagaagt gcagatgcac ggtggcgttg tccgtaccgc gtccggtggg
    13861 ctggcgggca gtacgctgtc tgttgatgcg gcagtgcgca atatggtcga gttgacgggc
    13921 gtaacgcctg cggaagccat tcatatggca tcgctgcatc cggcgcgaat gctgggtgtt
    13981 gatggtgttc tgggatcgct taaaccgggc aaacgcgcca gcatcgttgc gctggatagc
    14041 gggctgcatg tgcaacaaat ctggattcag ggtcaattag cttcgttttg atggtttgct
    14101 cctttattgg gccttcactt cccccgtaag gcctttcttt ttctttcgtt ttgatctgtg
    14161 cagcggtgtc ggatgcgacg ctaacgcgtc ttatgcgacc tacggtacgc cgcatccggc
    14221 aatcggtgaa catagcgacg tcattacgca attctttaat cttcccttgt cttttctttc
    14281 aatgatcaca gatttcgttt tatttccttt ttctccattg aactttcggt ttcttttcta
    14341 tagattttaa tcaacgaaag acatcaccaa gtgaaatgaa acgaaaggca agtgaaagcg
    14401 acaacgcccg acgtcaagtt catcagacta aggattgagt tatgccagaa aattacaccc
    14461 ctgctgccgc cgcatccggt acatggactg aagaagagat ccgccatcag cctcgcgcat
    14521 ggatccgttc actcaccaac atcgacgcgc tacgttccgc gctcaataac ttccttgaac
    14581 cgttactgcg caaagagaat ctgcggatca tcctgaccgg agccggaacc tcggcattta
    14641 tcggtgacat catcgcgccg tggctcgcca gccataccgg taaaaacttc agcgccgtac
    14701 cgaccaccga tctggtcact aatccgatgg actacctgaa cccagcccat ccgctgctgt
    14761 tgatctcctt cggtcgatcc ggcaacagcc cggaaagcgt cgcagccgtg gaactggcaa
    14821 atcaatttgt accggaatgc tatcacctgc cgatcacctg caacgaagcg ggcgctcttt
    14881 accaaaacgc gatcaacagc gataacgcgt ttgccgtgct gatgcccgca gaaacgcacg
    14941 atcgcggctt tgcgatgacc agcagcatta ccaccatgat ggccagctgc ctcgcggttt
    15001 tcgcacctga gacgatcaac agccaaacct tccgcgacgt ggcggatcgt tgccaggcga
    15061 tcctgacctc actgggcgat ttcagcgaag gtgtgtttgg ttacgcaccg tggaaacgga
    15121 tcgtttatct cggcagcggt ggcttacagg gcgcagcacg cgagtcggcg ctgaaagtgc
    15181 tggaactgac ggcgggtaaa ctggcggcct tttatgattc tccaaccgga ttccgtcatg
    15241 gaccaaaatc gctggtcgat gacgaaacgc tggtggtggt atttgtctcc agccaccctt
    15301 acacccgtca gtatgatctt gatctgctgg ctgaacttcg ccgtgacaac caggcaatgc
    15361 gtgtaatcgc catcgccgcg gaaagcagcg acatcgtcgc tgccggtcca catatcatcc
    15421 tgccaccgtc acgtcacttt atcgacgttg agcaggcatt ttgcttcctg atgtacgccc
    15481 agacgtttgc actgatgcag tcgctgcaca tgggcaatac gccggatacc ccatcagcca
    15541 gtggcaccgt taaccgcgtg gtgcaaggcg taatcattca tccgtggcag gcataagagg
    15601 atcgcattat gagcattatc tccactaaat atctgttaca ggacgcccag gccaatggct
    15661 acgcggtgcc tgcctttaac attcataacg ccgagacgat ccaggcgatc ctcgaagtgt
    15721 gcagtgaaat gcgatcgccg gtgatcctcg ccggaacgcc ggggaccttt aaacatatcg
    15781 cgctggaaga gatctacgcc ctgtgcagcg cctattccac aacctacaac atgccactgg
    15841 cgctgcatct cgaccaccac gaatcgctgg atgatattcg ccgtaaagtc cacgcaggtg
    15901 tgcgcagtgc aatgatcgac ggcagccact tcccgtttgc cgagaacgtg aagctggtga
    15961 aatcggttgt tgacttctgc cactcacagg attgcagcgt ggaagcagaa ctgggccgcc
    16021 tgggcggtgt tgaagatgac atgagcgttg acgccgaaag tgcattcctg accgatccac
    16081 aagaagctaa acgctttgtc gaactgactg gcgtcgacag cctggcggta gcgattggta
    16141 cggcgcacgg cttatacagc aaaacgccga agattgattt ccagcggctg gcggaaattc
    16201 gtgaagtggt ggatgttcct ctggtgctac atggtgccag cgatgttccg gatgaatttg
    16261 tccgtcgcac tattgaactt ggcgtcacaa aagtgaacgt tgccacagaa ttaaaaatag
    16321 ccttcgccgg cgcggttaaa gcctggtttg cggaaaatcc gcagggtaat gatcctcgtt
    16381 attatatgcg cgtcggaatg gatgcgatga aagaagttgt cagaaataaa attaatgtct
    16441 gtggttcag
//
LOCUS       DAZKFU020000094        16226 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZKFU020000094 DAZKFU020000000
VERSION     DAZKFU020000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.095"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHOQQ_RS12240"
                     /old_locus_tag="pgaptmp_002448"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHOQQ_RS12240"
                     /old_locus_tag="pgaptmp_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGU4470687.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHOQQ_RS12245"
                     /old_locus_tag="pgaptmp_002449"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHOQQ_RS12245"
                     /old_locus_tag="pgaptmp_002449"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGU4470688.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHOQQ_RS12250"
                     /old_locus_tag="pgaptmp_002450"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHOQQ_RS12250"
                     /old_locus_tag="pgaptmp_002450"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGU4470689.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNTGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHOQQ_RS12255"
                     /old_locus_tag="pgaptmp_002451"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHOQQ_RS12255"
                     /old_locus_tag="pgaptmp_002451"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGU4470690.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHOQQ_RS12260"
                     /old_locus_tag="pgaptmp_002452"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHOQQ_RS12260"
                     /old_locus_tag="pgaptmp_002452"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGU4470691.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="ACHOQQ_RS12265"
                     /old_locus_tag="pgaptmp_002453"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="ACHOQQ_RS12265"
                     /old_locus_tag="pgaptmp_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGU4470692.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11351
                     /gene="ftsK"
                     /locus_tag="ACHOQQ_RS12270"
                     /old_locus_tag="pgaptmp_002454"
     CDS             7245..11351
                     /gene="ftsK"
                     /locus_tag="ACHOQQ_RS12270"
                     /old_locus_tag="pgaptmp_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGU4470693.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     DITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11506..12117
                     /gene="lolA"
                     /locus_tag="ACHOQQ_RS12275"
                     /old_locus_tag="pgaptmp_002455"
     CDS             11506..12117
                     /gene="lolA"
                     /locus_tag="ACHOQQ_RS12275"
                     /old_locus_tag="pgaptmp_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGU4470694.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12128..13471
                     /gene="rarA"
                     /locus_tag="ACHOQQ_RS12280"
                     /old_locus_tag="pgaptmp_002456"
                     /gene_synonym="mgsA"
     CDS             12128..13471
                     /gene="rarA"
                     /locus_tag="ACHOQQ_RS12280"
                     /old_locus_tag="pgaptmp_002456"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGU4470695.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIILPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13562..14854
                     /gene="serS"
                     /locus_tag="ACHOQQ_RS12285"
                     /old_locus_tag="pgaptmp_002457"
     CDS             13562..14854
                     /gene="serS"
                     /locus_tag="ACHOQQ_RS12285"
                     /old_locus_tag="pgaptmp_002457"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGU4470696.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(15095..15235)
                     /locus_tag="ACHOQQ_RS12290"
                     /old_locus_tag="pgaptmp_002458"
     CDS             complement(15095..15235)
                     /locus_tag="ACHOQQ_RS12290"
                     /old_locus_tag="pgaptmp_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4470697.1"
                     /translation="MSQKSLMAITFCVTAILIIWMLHGSLCEIRMNIWGAEFAAFLQC
                     KQ"
     gene            complement(15564..15704)
                     /locus_tag="ACHOQQ_RS12295"
                     /old_locus_tag="pgaptmp_002459"
     CDS             complement(15564..15704)
                     /locus_tag="ACHOQQ_RS12295"
                     /old_locus_tag="pgaptmp_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4470698.1"
                     /translation="MPQKTIIVSILCLTMLLAIWMIHRSPCEFRLNIMWSEFAAFLQC
                     KQ"
     gene            complement(15839..16141)
                     /locus_tag="ACHOQQ_RS12300"
                     /old_locus_tag="pgaptmp_002460"
     CDS             complement(15839..16141)
                     /locus_tag="ACHOQQ_RS12300"
                     /old_locus_tag="pgaptmp_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4470699.1"
                     /translation="MFHCPFCGASARIRTSKAANDTGTIRYKYYQCNNLECSATFSTM
                     ESVHRIIGQPQLIFHDKDIPRETMRKSHQGRSQLTLQLEDCGMALKPVHYARVPFG"
BASE COUNT         4061 a         4168 c         4263 g         3734 t
ORIGIN      
        1 ttgttgcctg acctcatggg gggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattacccc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataattc agataatccc
      841 gacggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gcccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atcaccgcca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttctc cggtgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaacgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacctc cggtttttga tccgttaagt cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cctgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccag gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcgattgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgct gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgcaat gttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtaatg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcgaccaac
     3841 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc agcggatcgg tcaatgctaa
     3961 ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactatc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gtcgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca tcccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgctcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tgtcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaactccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctg aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatccggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcctaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg aatatgaaga tgacgaagag tatgaagatg
     7921 aaaaccacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gatgctgcgc
     8041 tgttctccgg taagcggatg gatgatgaag aagatattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc tgacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaat ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagtcacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccgcagcagc
     8461 cgtattatgc acctgcagct gaacaacctg cgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattacgcaa ctgcgccgga acagcctgcg caacagccgt
     8581 attatgcccc tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc gaagagcagc
     8641 aatccacttt tgctccacag tctacatacc agactgagca aacttatcag cagccagccg
     8701 ctcaggagcc gttgtaccaa cagccgcaac ctgttgaaca gcagcctgtt gtggagcctg
     8761 aacccgttgt agaagagaca aaacccgcgc gtccgccgct ttactacttt gaagaagtgg
     8821 aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg attccagaac
     8881 cggttaaaga accagaaccg atcaaatctt cgctgaaagc accttctgtt gcagcagtac
     8941 ctccagtaga agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac
     9001 tggcgacggg ggctgccgca accgttgccg cgccagtctt cagtctggca aatagcggtg
     9061 gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt gccacgaccg aaacgtatcc
     9121 gcgtgccaac tcgtcgtgaa ttggcgtctt acggtattaa gctgccctca cagcgtgcgg
     9181 cggaagaaaa agcccgtgaa gcccagcgca atcagtacga ttctggcgat cagtacaacg
     9241 atgatgaaat cgatgcgatg cagcaggatg aactggcacg tcagttcgcc cagacacagc
     9301 agcaacgcta tggcgaacag tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg
     9361 ctgcggcaga ggctgaactg gctcgtcagt ttgcgcaaac tcaacaacaa cgttattccg
     9421 gcgaacaacc ggctggggcg aatccgttct cgctggatga ttttgaattt tcgccaatga
     9481 aagcgttgct ggatgatggt ccacacgaac cgttgtttac gccaattgtt gaacctgtac
     9541 agcagccgca acaaccggtt gcaccgcagc cgcagtatca gcagccgcaa cagccggttg
     9601 caccgcagca gcagtatcag cagccacaac agccggttgc gccacagcag caatatcagc
     9661 agccgcaaca gccggttgca ccgcagccgc agtatcaaca gccgcaacaa ccagttgcgc
     9721 cgcagccgca gtatcaacag ccgcaacaac cggttgcacc gcagccgcag tatcagcagc
     9781 cacaacagcc agttgcgcca cagccgcagg ataccctgct tcatccgctg ttgatgcgta
     9841 atggcgacag ccgtccgttg cacaaaccga cgacgccgct gccttctctg gatttgctga
     9901 caccgccgcc gagcgaagtg gagccggtag atacctttgc gcttgaacaa atggcgcgtc
     9961 tggtggaagc gcgtctggct gatttccgta ttaaagccga tgtcgtcaat tactctccgg
    10021 ggccagttat cactcgcttt gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt
    10081 ctaacttgtc acgggacctt gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag
    10141 ttattcctgg caaaccctat gtaggtctgg agttaccaaa taaaaaacga caaaccgttt
    10201 atctgcgcga agttttggat aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg
    10261 tgctgggtaa agatatcgcc ggtgagccgg tggttgccga tctggcgaaa atgccgcact
    10321 tgttggttgc ggggactacc ggttccggta aatctgtcgg tgtgaacgcg atgatcctga
    10381 gcatgcttta taaagcacag ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc
    10441 tggagctttc ggtttatgaa ggcattccgc acctgttaac ggaagtcgtt actgatatga
    10501 aagatgccgc caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgg tataaactga
    10561 tgtctgcgct gggtgtgcgt aatctggcgg gttataacga aaaaattgct gaagccgatc
    10621 gcatgatgcg tccgattcca gacccgtact ggaagccggg tgacagtatg gatgcccagc
    10681 atccggtgct gaaaaaagaa ccatacattg tcgtgttggt tgacgaattt gccgacctga
    10741 tgatgacggt aggtaaaaaa gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg
    10801 ccgcgggtat ccacctcgta ctggcaactc agcgtccatc ggttgatgtt attactggtc
    10861 tgattaaagc gaatattccg acccgtatcg cctttaccgt atccagtaag attgactcac
    10921 gtaccattct tgatcaggct ggcgcggaat cactgctggg tatgggggat atgctctact
    10981 ctgggccgaa ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag
    11041 ttcatgccgt ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtt gatggcatca
    11101 cctccgacag cgaaagcgaa ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc
    11161 cgttgttcga tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca atttctggcg
    11221 tacaacgtca gttccgcatt ggttataacc gtgcagcgcg tattatcgaa cagatggaag
    11281 cgcaggggat tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg gccccaccgc
    11341 cgtttgacta attaatgcat tgccggataa ggcgcggtag cgtcgcatcc ggcactctat
    11401 caactgaaaa ttcagtattt tcttctttcc tcaagctgat tattagcctg gaatagagag
    11461 tagagggaac tcccgatcgg gagtgacgta atttgaggaa taatgatgaa aaaaattgcc
    11521 atcacctgtg cattactctc aagcttagta gcaagcagcg tttgggctga tgccgcaagc
    11581 gatctgaaaa gccgcctgga taaagtcagc agcttccacg ccagcttcac acaaaaagtg
    11641 actgacggta gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca
    11701 aacttattca actggcatat gacacaacct gatgaaagca ttctggtttc tgacggtaaa
    11761 acactgtggt tctataaccc gttcgttgag caagctacgg caacctggct gaaagatgcc
    11821 accggtaata cgccgtttat gctgattgcc cgcaaccagt ccagcgactg gcagcagtac
    11881 aatatcaaac agaatggcga tgactttgtc ctgacgccga aagccagcaa tggcaatctg
    11941 aagcagttca ccattaacgt gggacgtgat ggcacaatcc atcagtttag cgcggtggag
    12001 caggacgatc agcgcagcag ttatcaactg aaatcccagc aaaatggggc tgtggatgca
    12061 gcgaaattta ccttcacccc gccgcaaggc gtcacggtag atgatcaacg taagtagagg
    12121 cacctgagtg agcaatctgt cgctcgattt ttcggataat acttttcaac ctctggccgc
    12181 gcgtatgcgg ccagaaaatt tagcacagta tatcggtcag caacatttgc tggctgcggg
    12241 gaagccgttg ccgcgcgcta tcgaagccgg gcatttacat tctatgatcc tctgggggcc
    12301 accgggtacc ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga acgctgatgt
    12361 ggaacgtatt tctgccgtca cctctggtgt gaaagagatt cgcgaggcga tcgagcgcgc
    12421 ccggcaaaac cgcaatgcag gtcgccgcac tattcttttt gttgacgaag ttcaccgttt
    12481 caacaaaagc cagcaggatg catttctgcc acatattgaa gacggcacca tcacttttat
    12541 tggcgcaacc actgaaaacc cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg
    12601 tgtttatctg ttgaaatccc tgagtacaga ggatattgag caagtactaa ctcaggcgat
    12661 ggaagacaaa acccgtggct atggtggtca ggatattatt ctgccagatg aaacacgacg
    12721 cgccattgct gaactggtga atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat
    12781 ggcggatatg gccgaagtcg atgatagcgg taagcgggtc ctgaagcctg aattactgac
    12841 cgaaatcgcc ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct tttacgatct
    12901 gatttccgca ctgcataagt cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta
    12961 tgcgcgaatt attaccgctg gtggcgatcc gttatatgtc gcgcgtcgct gtctggcgat
    13021 tgcgtctgaa gacgtcggta atgccgatcc acgggcgatg caggtggcaa ttgcggcctg
    13081 ggattgcttt actcgcgttg gcccggcgga aggtgaacgc gccattgctc aggcgattgt
    13141 ttacctggcc tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc
    13201 cgatgctcgc gaacgcccgg attatgacgt gccggttcat ttgcgtaatg cgccgacgaa
    13261 attaatgaag gaaatgggct acgggcagga atatcgttac gctcatgatg aagcaaacgc
    13321 ttatgctgcc ggtgaggttt acttcccgcc ggaaatagca caaacacgct attatttccc
    13381 gacaaacagg ggccttgaag gcaagattgg cgaaaagctc gcctggctgg ctgaacagga
    13441 tcaaaatagc cccataaaac gctaccgtta atgttatcgt tgcggtaatg ttgttactgt
    13501 atccctgtgg tcgcaggctg tggccacatc tcccatttaa ttcgataagc acaggataag
    13561 catgctcgat cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa aactggcacg
    13621 ccggggcttt aagctggatg tagataagct gggcgctctt gaagagcgtc gtaaagtatt
    13681 gcaggtcaaa acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca
    13741 ggcgaaagcg cgcggggaag atatcgagcc tttacgtctg gaagtgaaca aactgggcga
    13801 agagctggat gcagcaaaag ccgagctgga tgctttacag gctgaaattc gcgatatcgc
    13861 gctgaccatc cctaacctgc ctgcagatga agtgccggta ggtaaagacg aaaatgacaa
    13921 cgttgaagtc agccgctggg gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt
    13981 gacgctgggt gaaatgcact ctggcctcga ctttgcagct gcagttaagc tgactggttc
    14041 ccgctttgtg gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat
    14101 gctggatctg cataccgaac agcatggcta cagtgagaac tatgttccgt acctggttaa
    14161 ccaggacacg ctgtacggta cgggtcagct gccgaaattt gctggcgatc tgttccatac
    14221 tcgtccgctg gaagaagaag cagacaccag taactatgcg ctgatcccaa cggcagaagt
    14281 tccgctgact aacctggtgc gcggtgaaat catcgatgaa gatgatctgc caattaagat
    14341 gaccgcccac accccatgct tccgttctga agccggttca tatggtcgtg acacccgtgg
    14401 tctgatccgt atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc gcccggaaga
    14461 ctcaatggcg gcgctggaag agatgactgg tcatgcagaa aaagtcctgc agttgctggg
    14521 cctgccgtac cgtaaaatca tcctttgcac tggcgacatg ggctttggcg cttgcaaaac
    14581 ttacgacctg gaagtatgga tcccggcaca gaacacctac cgcgagatct cttcctgctc
    14641 caacgtttgg gatttccagg cacgtcgtat gcaggcacgt tgtcgcagca aatcggacaa
    14701 gaaaacccgt ctggttcata ccctgaacgg ttctggtctg gctgttggtc gtacgctggt
    14761 tgcggttatg gaaaactatc agcaggctga tggtcgtatt gaagtaccag aagttctgcg
    14821 tccgtatatg aacggactgg aatatattgg ctaataccta atttttctga atctaaaaag
    14881 cgcctgcggg cgctttttgc atgaggttaa ttaactgaaa ataaaaggga tttattttca
    14941 ggtgtccata taatgcccat ctttgaaaca aaaaaagccc gcagttttta cgctgcgggc
    15001 tttatttttc tttgtgactt gcgtgtgagg gtgctacttt cggccttacc ctgacaaccg
    15061 attgacgggg gattactccc ccgtcgcggt ttccttactg cttacactgt aagaacgccg
    15121 caaactccgc tccccagatg ttcatccgta tttcacacag cgaaccatgt aacatccaga
    15181 tgatgaggat tgccgtcacg cagaacgtga tggccataag cgatttttgc gacatagcgc
    15241 ttgctccttt gttggagagg cgctaacctg tcacttgcta aggttagatg gttagggcct
    15301 cgggttaacg tttaagttga ctcggggcct ttccacatcc ggccttcagg taatccttcc
    15361 agccgtcagc cgaaaggcac ccacgtgtaa tctactgatt tccctttgtg cgcgcaatgc
    15421 tcagtgttga aactgtttag gggggcggca aaaaagcccg catattgcgg gcaaatacgt
    15481 ctgtaatcca atggagaaag gggtgccata tcgactgaac ccgacaaccg gatagcgggg
    15541 gctttcaccc ccgctgcggt ttcttactgt ttacactgca agaacgccgc aaattctgac
    15601 cacatgatgt taagtctgaa ctcacacggc gaacggtgta tcatccagat ggccaacaac
    15661 atggttaagc ataaaatgct cacgatgatc gttttttgcg gcatagcgct tgactccttt
    15721 tacagggagg cgctaacctt ccacttgtct agggtaggaa tggttagggc ctcgggttaa
    15781 acaatatgtt tgactcgggg cctttccaca tccggccttc aggttttcct tccagccgtc
    15841 agccgaaagg cacccgcgcg taatgtactg gttttaaagc cataccgcaa tcctccagtt
    15901 gtagcgttaa ctgactgcgt ccctgatggg atttgcgcat tgtctcccgt ggaatatctt
    15961 tatcgtggaa gatcaactga ggctggccaa taattctgtg cacactttcc attgttgaaa
    16021 atgtggcgct gcactcaagg ttattgcact gatagtattt gtagcggatg gtgccggtat
    16081 cattggcggc tttactggta cggatccggg cagatgcgcc gcagaatgga caatgaaaca
    16141 tcgatatacc ccctggctga actggggttg aactcgtcgc tattctaacc attgctttcc
    16201 ttttcggcta tccactcggg tattct
//
LOCUS       DAZKFU020000095        16198 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZKFU020000095 DAZKFU020000000
VERSION     DAZKFU020000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.096"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..153
                     /locus_tag="ACHOQQ_RS12305"
                     /old_locus_tag="pgaptmp_002461"
     CDS             <1..153
                     /locus_tag="ACHOQQ_RS12305"
                     /old_locus_tag="pgaptmp_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4470700.1"
                     /translation="LPKRPNRAILIDHVGLNGSRAFRWSHFKLTNEQFLKIIELGKIN
                     ESFIIH"
     gene            131..508
                     /locus_tag="ACHOQQ_RS12310"
                     /old_locus_tag="pgaptmp_002462"
     CDS             131..508
                     /locus_tag="ACHOQQ_RS12310"
                     /old_locus_tag="pgaptmp_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU4470701.1"
                     /translation="MKVLLSIKPEFAEKILNGTKRFEFRKGIFKNPQISTVVIYATMP
                     LGKVVGQFRIESILSDEPESLWKKTEKHAGISKQFYDSYYSGREKAYAIKIGEVERYK
                     EPIPISALGSNIKPPQSYLYLPA"
     gene            989..1195
                     /locus_tag="ACHOQQ_RS12315"
                     /old_locus_tag="pgaptmp_002463"
     CDS             989..1195
                     /locus_tag="ACHOQQ_RS12315"
                     /old_locus_tag="pgaptmp_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HGU4470702.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            1270..1566
                     /locus_tag="ACHOQQ_RS12320"
                     /old_locus_tag="pgaptmp_002464"
     CDS             1270..1566
                     /locus_tag="ACHOQQ_RS12320"
                     /old_locus_tag="pgaptmp_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4470703.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPLHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1572..2357
                     /gene="bet"
                     /locus_tag="ACHOQQ_RS12325"
                     /old_locus_tag="pgaptmp_002465"
     CDS             1572..2357
                     /gene="bet"
                     /locus_tag="ACHOQQ_RS12325"
                     /old_locus_tag="pgaptmp_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU4470704.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            2354..3034
                     /locus_tag="ACHOQQ_RS12330"
                     /old_locus_tag="pgaptmp_002466"
     CDS             2354..3034
                     /locus_tag="ACHOQQ_RS12330"
                     /old_locus_tag="pgaptmp_002466"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU4470705.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVTPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3031..3189
                     /locus_tag="ACHOQQ_RS12335"
                     /old_locus_tag="pgaptmp_002467"
     CDS             3031..3189
                     /locus_tag="ACHOQQ_RS12335"
                     /old_locus_tag="pgaptmp_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HGU4470706.1"
                     /translation="MTHPHDNIRVGAITFVYSITKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKQEDI"
     gene            3186..4250
                     /locus_tag="ACHOQQ_RS12340"
                     /old_locus_tag="pgaptmp_002468"
     CDS             3186..4250
                     /locus_tag="ACHOQQ_RS12340"
                     /old_locus_tag="pgaptmp_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HGU4470707.1"
                     /translation="MSQVGNHSFEFPASQGVQGGTVTLFLTIPGRSLARFLASDNYGH
                     TLARSQREINPNRVRKFLNYLTNADSRNEPFIIPPLVGNCDSNIEFVPFGNTNVGIAR
                     IPLDAEIKLFDGQHRAAGIEIFCRSSPSTLMVPMMLTMNLPLKTRQQFFSDINNNVSK
                     PSATINMAYNGRDDIAQGMISFLTQHTVFADITDFEHNVVPLKSNMWVSFKALTDATS
                     KFARNGNQQLEMGYIESVWEAWITLTQIDSIRHGVHHATYKRDYIQFHGVMINAFGFA
                     VQQMMVNHSIAEITSMIEKLCATTSSAEREDFFLMDNWAGICTKASQEKLSVIANVAA
                     QKAAANRLIQAFTKGSLETT"
     gene            4404..4625
                     /locus_tag="ACHOQQ_RS12345"
                     /old_locus_tag="pgaptmp_002469"
     CDS             4404..4625
                     /locus_tag="ACHOQQ_RS12345"
                     /old_locus_tag="pgaptmp_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4470708.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(4836..5438)
                     /locus_tag="ACHOQQ_RS12350"
                     /old_locus_tag="pgaptmp_002470"
     CDS             complement(4836..5438)
                     /locus_tag="ACHOQQ_RS12350"
                     /old_locus_tag="pgaptmp_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470709.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            5681..6025
                     /locus_tag="ACHOQQ_RS12355"
                     /old_locus_tag="pgaptmp_002471"
     CDS             5681..6025
                     /locus_tag="ACHOQQ_RS12355"
                     /old_locus_tag="pgaptmp_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470710.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            6149..6349
                     /gene="torI"
                     /locus_tag="ACHOQQ_RS12360"
                     /old_locus_tag="pgaptmp_002472"
     CDS             6149..6349
                     /gene="torI"
                     /locus_tag="ACHOQQ_RS12360"
                     /old_locus_tag="pgaptmp_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HGU4470711.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            6593..6766
                     /locus_tag="ACHOQQ_RS12365"
                     /old_locus_tag="pgaptmp_002473"
                     /pseudo
     CDS             6593..6766
                     /locus_tag="ACHOQQ_RS12365"
                     /old_locus_tag="pgaptmp_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6861..8108)
                     /locus_tag="ACHOQQ_RS12370"
                     /old_locus_tag="pgaptmp_002474"
     CDS             complement(6861..8108)
                     /locus_tag="ACHOQQ_RS12370"
                     /old_locus_tag="pgaptmp_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HGU4470712.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8180..9094)
                     /locus_tag="ACHOQQ_RS12375"
                     /old_locus_tag="pgaptmp_002475"
     CDS             complement(8180..9094)
                     /locus_tag="ACHOQQ_RS12375"
                     /old_locus_tag="pgaptmp_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HGU4470713.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDERGMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            9310..10743
                     /gene="cscA"
                     /locus_tag="ACHOQQ_RS12380"
                     /old_locus_tag="pgaptmp_002476"
     CDS             9310..10743
                     /gene="cscA"
                     /locus_tag="ACHOQQ_RS12380"
                     /old_locus_tag="pgaptmp_002476"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGU4470714.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNCKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(10751..11746)
                     /locus_tag="ACHOQQ_RS12385"
                     /old_locus_tag="pgaptmp_002477"
     CDS             complement(10751..11746)
                     /locus_tag="ACHOQQ_RS12385"
                     /old_locus_tag="pgaptmp_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4470715.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            11977..12195
                     /locus_tag="ACHOQQ_RS12390"
                     /old_locus_tag="pgaptmp_002478"
                     /pseudo
     CDS             11977..12195
                     /locus_tag="ACHOQQ_RS12390"
                     /old_locus_tag="pgaptmp_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            12213..13541
                     /gene="dsdA"
                     /locus_tag="ACHOQQ_RS12395"
                     /old_locus_tag="pgaptmp_002479"
     CDS             12213..13541
                     /gene="dsdA"
                     /locus_tag="ACHOQQ_RS12395"
                     /old_locus_tag="pgaptmp_002479"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGU4470716.1"
                     /translation="MENAKMNSLIAQYPLVKVLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELIAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMT
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(13649..15187)
                     /gene="emrY"
                     /locus_tag="ACHOQQ_RS12400"
                     /old_locus_tag="pgaptmp_002480"
     CDS             complement(13649..15187)
                     /gene="emrY"
                     /locus_tag="ACHOQQ_RS12400"
                     /old_locus_tag="pgaptmp_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGU4470717.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15187..>16198)
                     /gene="emrK"
                     /locus_tag="ACHOQQ_RS12405"
                     /old_locus_tag="pgaptmp_002481"
     CDS             complement(15187..>16198)
                     /gene="emrK"
                     /locus_tag="ACHOQQ_RS12405"
                     /old_locus_tag="pgaptmp_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGU4470718.1"
                     /translation="GNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALN
                     KAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETL
                     EHTKDTLISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDI
                     KSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIIS
                     DLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHP
                     LRIGLSMTATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNG
                     QL"
BASE COUNT         4662 a         3887 c         3651 g         3998 t
ORIGIN      
        1 ttgccaaaga gacccaatcg tgctatttta atagatcatg tggggctaaa tggttcgcgt
       61 gcattccgat ggagtcactt taaactcaca aatgagcagt tcttaaagat catcgagtta
      121 ggcaagataa atgaaagttt tattatccat taagcctgag tttgcagaaa aaatattgaa
      181 cggaacaaag cggttcgagt ttcgtaaagg tatattcaaa aatccgcaaa ttagcaccgt
      241 tgttatttat gccacgatgc cattaggtaa agttgttggt caattccgta ttgaatcaat
      301 actaagtgac gaaccggaat ctctttggaa aaagacggaa aaacacgcag gtatttctaa
      361 gcaattttat gactcatatt attcaggtag agaaaaggcc tacgcaataa aaattggtga
      421 agtggaaaga tataaagaac caattcctat ctctgctcta ggtagtaata ttaagccacc
      481 acaatcatat ctttacctac ctgcgtaaga atcccggcca ccgtgccggg ttttcttttg
      541 tcccctcccc tcatcacaca aaccgttcga aaaaccacca caacctccct tcagttatcg
      601 ctatgcgatg caagtcacaa aattaattct ttttgctatc aaacagttaa tatcaaaaca
      661 catcaatcaa tatcaataag tattgatgca accaatagca atagctatta tcaccatgtc
      721 gcaacaacac aacgatacgg caatcacctg attcaccgtt gcgatgaccg cttagatccg
      781 cagcttgaat ttcagcaggc tccggggagt gcgaggggtg aagcggacgc gtgaacgtcg
      841 gtgtgaccag ctgaaatcaa ctcaacacct catacctcag tcgcttcaac gaggcggctt
      901 agttatgaca accggcggcc atccaccgcc tgaatacgcg cagaagtctc tatatgttca
      961 gcagcccagc ttacgggcag gagtttttat ggttcatcaa cattacggaa cgcagaccgt
     1021 taatcgcggt gcggtcatgc caggaatgct ggtcaaacac aaagatggta cctggactgc
     1081 atcagctaat ttacgcggac ggctttatct gcatcgcggc atcgagcgca cttatacccg
     1141 tgatttgctc gtggaagttt ttctcgacgg acgcggcaac ggcctgaatc actaatcccc
     1201 tttcctgttt acctaatcag cccggcattt cgcaggcgat attttcacag ctatttcagg
     1261 agttcagcca tgaacgctta ttacattcag gatcgtcttg aggctcagag ctgggcgcgt
     1321 cactaccagc agatcgcccg tgaagagaaa gaggcagaac tggcagacga catggaaaaa
     1381 ggcctgcccc tgcacctgtt tgaatcgcta tgcatcgatc atttgcaacg ccacggggcc
     1441 agcaaaaaag ccattacccg tgcgtttgat gacgatgttg agtttcagga gcgcatggca
     1501 gaacacatcc ggtacatggt tgaaaccatt gctcaccacc aggttgatat tgattcagag
     1561 gtataaaacg gatgagtaca gcactcgcaa cgctggctgg gaagctggct gaacgtgtcg
     1621 gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagaca gcatttaaag
     1681 gcgatgccag cgatgcgcag ttcatcgcat tgctgatcgt cgccaaccaa tacggcctta
     1741 atccgtggac gaaagaaatt tacgccttcc ctgataagca gaacggcatc gttccggtgg
     1801 tgggcgttga tggctggtcc cgcatcatca acgaaaacca gcagtttgac ggcatggact
     1861 ttgaacagga caacgaatcc tgcacatgcc ggatttaccg caaagaccgc aatcatccga
     1921 tctgcgttac cgagtggatg gatgaatgcc gccgcgaacc attcaaaacc cgcgaaggca
     1981 gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat gttacggcat aaagccatga
     2041 ttcagtgtgc ccgtctcgcc ttcggatttg ctggtatcta tgacaaggat gaagccgagc
     2101 gcattgtcga aaataccgca tacactgcag aacgtcagcc ggaacgcgac atcactccgg
     2161 ttaacgatga aaccatgcag gagattaaca ctctgctgat cgccctggat aaaacatggg
     2221 atgacgactt attgccgctc tgttcccaga tatttcgccg cgacattcgc gcatcgtcag
     2281 aactgacaca ggccgaagca gtgaaagctc ttggattcct gaaacagaaa gccactgagc
     2341 agaaggtggc agcatgacac cggacattat cctgcagcgt accgggatcg acgtgagagc
     2401 tgtcgaacag ggggatgatg cgtggcacaa attacggctc ggcgtcatca ccgcttcaga
     2461 agttcacaac gtgatagcaa agccccgctc aggaaagaag tggcctgaca tgaaaatgtc
     2521 ctacttccac accctgctgg ctgaggtttg caccggtgtg actccggaag ttaatgctaa
     2581 ggcgctggcc tggggaaaac agtacgagaa cgacgccaga actctgtttg aattcacttc
     2641 cggcgtgaat gttactgaat ccccgatcat ctatcgcgac gaaagtatgc gcaccgcctg
     2701 ctctcccgat ggtttatgca gtgacggcaa tggccttgag ctgaaatgcc cgtttacctc
     2761 ccgggatttc atgaagttcc ggctcggtgg tttcgaggcc ataaagtcgg cttacatggc
     2821 ccaggtgcag tacagcatgt gggtgacacg aaaagatgcc tggtactttg ccaactatga
     2881 cccgcgtatg aagcgtgaag gactgcatta tgtcgtggtt gagcgggatg aaaagtacat
     2941 ggcgagtttt gacgagatgg tgccggagtt catcgaaaaa atggacgagg cactggctga
     3001 aattggtttt gtatttgggg agcaatggcg atgacgcatc ctcacgataa tatccgggta
     3061 ggcgcgatca ctttcgtcta ctccattaca aagcgaggct gggtatttcc cggcctttct
     3121 gttatcagaa atccactgaa agcacagcgg ctggctgaga agataaataa taaacaggag
     3181 gatatatgag tcaggttggt aatcattcat tcgaatttcc ggcatcgcaa ggtgtacagg
     3241 gtggtactgt tacactcttc cttaccatac caggaagatc gctggctcgt ttcctcgctt
     3301 cagataatta cggccataca ctggcacgct ctcagcgaga aattaatcca aatcgagtac
     3361 gaaaattttt aaattatctc actaacgcag actcaagaaa tgagcctttt atcattcccc
     3421 ctctcgtagg taactgtgat tcgaatatag aatttgtacc gtttggcaac acaaatgttg
     3481 gtatagccag aattcccctc gacgccgaaa taaaactttt tgatggtcaa catcgtgcag
     3541 ctggcattga gatattttgc cgaagttccc catcaacgct catggttccc atgatgctta
     3601 caatgaatct gccgctaaaa acccggcagc agttcttttc ggacataaat aacaacgttt
     3661 ctaagccatc agcgaccatc aatatggcgt ataacggccg ggatgatatt gctcagggaa
     3721 tgatatcctt cctgacccaa catactgtat ttgccgatat aaccgatttt gaacacaacg
     3781 tagtgccatt aaaaagtaat atgtgggtga gtttcaaggc actcactgat gcaacgtcaa
     3841 agttcgctag gaacggcaat caacaacttg aaatgggata tatagaatct gtctgggagg
     3901 catggattac actaactcag attgactcaa tccgacatgg tgtacaccac gctacgtaca
     3961 agcgcgatta tattcagttc catggagtaa tgattaacgc tttcggtttt gcggttcaac
     4021 agatgatggt taatcattcc atcgcagaaa taacttctat gatcgaaaaa ctatgtgcaa
     4081 ctaccagctc tgcagaaaga gaggattttt ttctgatgga taactgggcg gggatctgca
     4141 cgaaagccag ccaggaaaaa ctatctgtta ttgccaatgt ggcagcgcag aaagcagcag
     4201 caaacagact gatacaagct tttaccaaag gaagtctgga aacaacttaa tgaatcaaca
     4261 ttgtctcata tcagcatgct gtacggcgtc tttaaggaac ggtgagcatg aaaaacaaaa
     4321 tcatcatgga gctacaggct ccttttttat tattcgcatt caccctcaag cgtattaacc
     4381 aacaattcag ggattaatga aagatggcag acatcattga ttcagcatca gaaattgaag
     4441 aattacagcg caacacagca ataaaaatgc gccgcctgaa ccaccaggct atatctgcca
     4501 ctcattgttg tgagtgtggc gatccgatag atgaacgaag acgcctggcc gttcagggtt
     4561 gtcggacttg tgcaagttgc caggaggatc tggaacttat cagtaaacag agaggttcga
     4621 agtgagcgaa attaactaga agccaaagat aaaatcatcg ctgagcagga gaaaatcgct
     4681 aacggagaaa agacagtaag tcagtatatg aaaaccgcat gatatcatca gataaaaacc
     4741 gatcgtaaag cgaaatatta ataccagaat aaacgagtcg aggtaaatta tattacctcg
     4801 ataaattaac taaaacttgc ccgctatata ctatctcatt cagtatcatc acgcgcggtc
     4861 tgtgcatatg tcactaccgc acctaatgta ttaattttct tttcaacata gataatatta
     4921 tcgtactcat aattgccata cggatagcaa atgcgaatat tctcatgtag atcggggtca
     4981 tccacctcag ctccagaaca actttttgaa ctaccggaag tataccgata cggtgcaaca
     5041 taagacgatg tctctccagg caaaaaataa gttagtgttg taaggggtat aatcagaaaa
     5101 aatccagaaa atatgcacat ccctgcataa accttaaggt atgctgacag actcttccag
     5161 ccgctttgtt ttactatccc cttcttaacc caaaacagag ataacagaaa agctattccc
     5221 atgctaaaca gaatgtaata gtgggatata ctctgattaa gaaacgtgac cctgtagata
     5281 tctgcccgcc accagaagaa aaggaaaata aagatcagcc ctgaaactgt catgcaaatc
     5341 aaataaggat acgaatcttt tttcatgttt agcgcccata aaatttttcc tgcaccggac
     5401 aaatttacca tccatttttt gcgcagaaaa tagctcatta cttactgcac aataatacac
     5461 aaaattgcgt aaattttttg catggatttt agctctttca gccgacgttt aaggggtaaa
     5521 tagcatttcc taaaagcaac tgcaccaacc caacagaatg ggctaccgct tacgttgaga
     5581 gcaaaaaagt gtatagcagc aatgaacagc atcctcgcac tgacggggat ttcttttatc
     5641 tgagctcgct acggcgagtt ttgttttatg gagacaagaa atatcagatt tggctatgaa
     5701 ggttttgaaa tggcaatcaa ctggcgatgt cggcatcagt agcgcaactc ttgcctcaat
     5761 cgcatgtggc ctgaaaaaga atatctatgg tcatcacttc ggcgctcccc atgacgcagc
     5821 agactttcgg cgatgcgttg cacttgttga gcagattcca gaaatcagag attcattcga
     5881 caaggttgca aagcgcgttc cggcattcaa aggaatcctc aacgaatggg attcactcgt
     5941 tgctctgttg aagtctgaaa tgaagacgta cgggaacaaa gcaccagaga cttacagaag
     6001 aatcagcgag ctacgcaagg actaacccgc ctcacactcg atgaggcatt ttcatttatc
     6061 aagatatcca gacctaccat cgccgcatca atgcggtttt tttattacct gatttgcagg
     6121 ttcgattccc tattcggaga tagcactcat gcaacacgaa ctacagcctg attccctggt
     6181 tgatttgaaa ttcatcatgg ccgatactgg cttcggtaaa accttcatct atgaccggat
     6241 taagtccgga gacctgccta aagccaaagt tatccacggg cgagcaagat ggttatatcg
     6301 tgaccattgt gaattcaaaa ataagctctt aagccgcgcc aatgggtaaa atagcgggta
     6361 aaatatttct cacatctaaa aaacaccatt ctaatcaacc ccctgccgcc tcaagtagat
     6421 gtctgcaggg gacaccagat acccttcaaa cgaaatctac cttcaccccg taaaagatgg
     6481 gtttggcagc acacttgccc tatatctact catttttact gcaacaggtt gaaatctcag
     6541 cactgtcaga aagcgctgat gactaaacag ccttgggccg ggcgatgtaa ccatcacaca
     6601 gaatcctgat agcgaaatat ggcgtgactc gatacttcac tccgcaatgc attccttgat
     6661 gaattcgcag gcccgtgata cacgggacag gtcgctgaat gacgacaatg tcctggaaat
     6721 cagcgaaccg cgtatccgaa gtacatttga gcgactgtac cagaacatag gtgattatta
     6781 gcagggctaa gcattttagt attattattt tccggttgag ggatatggag atatcgacaa
     6841 caaccggaaa aagtttacgt ctatattgct gaaggtacag gcgtttccat aactatttgc
     6901 tcgcgttttt tactcaggaa gaaaatgcca aatagcaaca tcaggcagac aatacccgaa
     6961 attgcgaaga aaactgtctg gtagcctgcg tggtcaaaga gtatcccagt cggcgttgaa
     7021 agcagcacaa tcccaagcga actggcaatt tgaaaaccaa tcagaaagat cgtcgacgac
     7081 aggcgcttat caaagtttgc cacgctgtat ttgaagacgg atatgacaca aagtggaacc
     7141 tcaatggcat gtaacagctt cactaatgaa ataatccagg ggttaacgaa cagcgcgcag
     7201 gaaaggatac gcaacgccat aatcacaaca ccgataagta atgcattttt tggccctacc
     7261 cgattcacaa agaaaggaat aatcgccatg cacagcgctt cgagtaccac ctggaatgag
     7321 ttgagataac catacaggcg cgttcctaca tcgtgtgatt cgaataaacc tgcataaaag
     7381 acaggaaaaa gttgttgatc aaaaatgtta tagaaagacc acgtccccac aataaatatg
     7441 acgaaaaccc agaagtttcg atccttgaaa actgcgataa aatcctcttt ttttacccct
     7501 cccgcatccg ccgctacgca ctggtgatcc ttatctttaa aacacatgtt gatcatcata
     7561 aatacagcgc caaatagcga gaccaaccag aagttgatat ggggactgat actaaaaaat
     7621 atgccggcaa agaacgcgcc aatagcatag ccaaaagatc cccaggcgcg cgctgttcca
     7681 tattcgaaat gaaaatttcg cgccattttt tcggtgaagc tgtcaagcaa accgcatccc
     7741 gccagatacc ccaagccaaa aaagagcgcc cccagaatta gacctacaga aaaattgctt
     7801 tgcagtaacg gttcataaac gtaaatcata aacggtccgg tcaagaccag gatgaaactc
     7861 atacaccaga tgagtggttt cttcagaccg agtttatcct gaacgatgcc gtagaacatc
     7921 ataaatagaa tgctggtaaa ctggttgacc gaataaagtg tacctaattc cgtccctgtc
     7981 aaccctagat gtcctttcag ccaaatagcg tataacgacc accacagcga ccaggaaata
     8041 aaaaagagaa atgagtaact ggatgcaaaa cgatagtacg catttctgaa tgggatattc
     8101 agtgccataa ttacctgcct gtcgttaaaa aattcacgtc ctatttagag ataagagcga
     8161 cttcgccgtt tacttctcac tattccagtt cttgtcgaca tggcagcgct gtcattgccc
     8221 ctttcgccgt tactgcaagc gctccgcaac gttgagcgag atcgataatt cgtcgcattc
     8281 ctctctcatc tgtagataat cccgtagagg acagacctgt gagtaacccg gcaacgaacg
     8341 catctcccgc ccccgtgcta tcgacacaat tcacagacat tccagcaaaa tggtgaactt
     8401 gtcctcgata acagaccacc accccttctg cacctttagt caccaacagc atggcgatct
     8461 catactcttt tgccagggcg catatatccc gatcgttctg tgtttttcca ctgattagtc
     8521 gccattcttc ttccgagagc ttgacgacat ccgccagttg tagcgcctgc cgcaaacaca
     8581 agcggagcaa atgctcgtct tgccatagat cttcacgaat attgggatcg aagctgacaa
     8641 aacctccggc atgccggatc gccgtcatcg cagtaaatgc gctggtacgc gaaggctcgg
     8701 cagacaacgc aattgaacag agatgtaacc attcgccatg tcgccagcag ggcaagtctg
     8761 tcgtctctaa aaaaagatcg gcactggggc gaaccataaa cgtaaatgaa cgttcccctt
     8821 gatcgttcag atcgacaagc accgtggatg tccggtgcca ttcatcttgc ttcagatacg
     8881 tgatatcgac tccctcagtt agcagagttc tttgcattaa cgcaccaaaa ggatcatcac
     8941 cgacccgacc tataaaccca cttattccgc ctaatctggc gattcccacc gcaacgttag
     9001 ctggcgcgcc gccaggacaa ggcagtaggc gcccgtctga ttctggcaag agatctacta
     9061 ccgcatcccc taaaacccat actttggctg acattttttt cccttaaatt catctgactt
     9121 acgcatagtg ataaacctct ttttcgcaaa atcgtcatag atttactaaa acatgcatat
     9181 tcgatcacaa aatgtcatag ttaacgttaa catttgtgat attcatcgca tttatgaaag
     9241 taagggactt tatttttata aaagttaacg ttaacaattc accaaatttg cttaaccagg
     9301 atgattaaaa tgacgcaatc tcgattgcat gcagcgcaaa acgcactagc aaaacttcat
     9361 gagcaccggg gtaacacttt ctatccccat tttcacctcg cgcctcctgc cgggtggatg
     9421 aacgatccaa acggcctgat ctggtttaac gatcgttatc acgcgtttta tcaacatcac
     9481 ccaatgagcg aacactgggg gccaatgcac tggggacatg ccaccagtga cgatatgatc
     9541 cactggcagc atgagcctat tgcgctagcg ccaggagacg ataatgacaa agacgggtgt
     9601 ttttcaggta gtgctgtcga tgacaatggt gtcctctcac ttatctacac cggacacgtc
     9661 tggctcgatg gtgcaggtaa tgacgatgca attcgcgaag tacaatgtct ggctaccagt
     9721 cgggatggta ttcatttcga gaaacagggt gtgatcctca ctccaccaga aggaatcatg
     9781 cacttccgcg atcctaaagt gtggcgtgaa gccgacacat ggtggatggt agtcggggcg
     9841 aaagacccag gcaacacggg gcagatcctg ctttatcgcg gcagttcatt acgtgaatgg
     9901 actttcgatc gcgtactggc ccacgctgat gcgggtgaaa gctatatgtg ggaatgtccg
     9961 gactttttca gccttggcga tcagcattat ctgatgtttt ccccgcaggg aatgaatgcc
    10021 gagggataca gttaccgaaa tcgctttcaa agtggcgtaa tacccggaat gtggtcgcca
    10081 ggacgacttt ttgcacaatc cgggcatttt actgaacttg ataacgggca tgacttttat
    10141 gcaccacaaa gctttgtagc gaaggatggt cggcgtattg ttatcggctg gatggatatg
    10201 tgggaatcgc caatgccctc aaaacgtgaa ggctgggcag gctgcatgac gctggcgcgc
    10261 gagctatcag agagcaattg caaactccta caacgcccgg tacacgaagc tgagtcgtta
    10321 cgccagcagc atcaatctat ctctccccgc acaatcagca ataaatatgt tttgcaggaa
    10381 aacgcgcaag cagttgagat tcagttgcag tgggcgctga agaacagtga tgccgaacat
    10441 tacggattac agctcggcac tggaatgcgg ctgtatattg ataaccaatc tgagcgactt
    10501 gttttgtggc ggtattaccc acacgagaat ttagacggct accgtagtat tcccctcccg
    10561 cagcgtgaca cgctcgccct aaggatattt atcgatacat catccgtgga agtatttatt
    10621 aacgacgggg aagcggtgat gagtagtcga atctatccgc agccagaaga acgggaactg
    10681 tcgctttatg cctcccacgg agtggctgtg gtgcaacatg gagcactctg gcaactgggt
    10741 taacataata tcaggtggaa caacggatca acagcgggca agggatccgc gtcacacttc
    10801 ccccttcacg accttcaata atatgcaatg cagcttcccg cccgataatg tcatgtggaa
    10861 gctgaattgt ggtcagcggc ggtaaaaaca gatgcccgac gccaaccaga ttatcaaagc
    10921 ccattacggc gacatcctgc gggatacgta cccccttagc caggagaacc tgataagcca
    10981 caaaggctgc gcgatcgtta ccacatatca gaacatcaaa atctggtttg cccgatttga
    11041 agtggtcatt gagtaaactt gcgagatcgg tgtagtgatc atcacctgtt gccatgtgaa
    11101 attgtttcac ctcagccaga tctcgtccag catcacgcca ggcctgctca aatccctgcc
    11161 gacgataccc tgttgccaac gcactttccg gtagccagaa gcataacggt tgacgatagc
    11221 ccgccgcgag caaatgctgt gttgattcat attgtgcagt gtaatcatca gggatataac
    11281 tgggtaacgc tgggtcatcc gccacacagt tcgccaatac aatattttca ccatacagag
    11341 actcaggcag cgtgatatgt cgcagcccca ttgtagtata gataatgcca tccggacggt
    11401 gggcaagcag ctgacgtgcc gcgcgggcag cgtcatcttc agaaaaaata ttgattaaaa
    11461 aactattcca gccgaactcg ctggcggttt gctcaatggc aagcagaata tcaacagaga
    11521 aaggagtggt agcagtgtcc tgcgccagca cggcgagagt cgacggctta cgtccttgag
    11581 cgcgcatctt acgggcggaa agatcaggaa cataattcag ggtctggatt gcctgcaata
    11641 cgcggtcacg cgttgcagga cgcacagatt ctgcattatg catcacccgg gagactgtca
    11701 tcatcgacac tcccgccagg cgtgcgacat cctttaatga agccataccc aagccgtttg
    11761 ccgtaaaacg ggcactgtag cagaaacaga cgtcactggc gagatccaac gccctatcac
    11821 ctgacacggc aatacaataa aaaataacaa taattcccgg ataattgtac ccaattccgc
    11881 ctctgttctc gccaacgagt aaccatatag gcaagtaagt aaaacatgcc aactggcatc
    11941 gccaacctgg agaagaaaat ataaatatgg atcattgtgc tgccgctgta tcccgacatc
    12001 agcccggaaa ttattgcgat tgctatcggt tcaggtgcga ttggctgcac gatcgttacg
    12061 gactcgcttt tctggctggt gaagcaatat tgcggcgcta cgctcaatga aacatttaaa
    12121 tactatacga cagcgacatt tatcgcttca gtcgtcgctc tggcgggcac attcctgctg
    12181 tcatttatca tctaagcgca aagagacgta ctatggaaaa cgctaaaatg aactcgctca
    12241 tcgcccagta tccgttggta aaggttctgg ttgctcttaa agaaaccacc tggtttaatc
    12301 ctggcacgac ctcattggct gaaggtttac cttatgttgg cctgaccgaa caggatgttc
    12361 aggacgccca tgcgcgctta tcccgttttg caccctatct ggcaaaagca tttcctgaaa
    12421 ctgctgccac tggggggatt attgaatcag aactgattgc cattccggct atgcaaaaac
    12481 ggctggaaaa ggaatatcaa caaccgatca gcgggcaact gctactgaaa aaagatagcc
    12541 atttgcccat ttccggctcc ataaaagcac gcggcgggat ttatgaagtc ctggcacacg
    12601 cagaaaaact ggctctggaa gcggggttgc tgacgcttga tgatgactac agcaaactgc
    12661 tttctccgga gtttaaacag ttctttagcc aatacagcat tgctgtgggc tcaaccggaa
    12721 atctggggtt atcaatcggc attatgagcg cccgcattgg ctttaaggtg acagtgcata
    12781 tgtctgctga tgcccgggca tggaaaaaag cgaaactgcg cagccatggc gttacggtcg
    12841 tggaatatga gcaagattat ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg
    12901 acccgaactg tttctttatt gatgacgaaa attcccgcac gttgttcctt gggtattccg
    12961 tcgctggcca gcgtcttaaa gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata
    13021 accctctgtt tgtctatctg ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg
    13081 ggcttaaact ggcgtttggc gatcatgttc actgcttttt tgccgaacca acgcactccc
    13141 cttgtatgtt gttaggcgtc catacaggat tacacgatca gatttctgtt caggatattg
    13201 gtatcgacaa ccttaccgca gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg
    13261 ggcgggcaat ggagcgtctg ctggatggct tctataccct tagcgatcaa accatgtatg
    13321 acatgcttgg ctggctggcg caggaagaag gtattcgtct tgaaccttcg gcactggcgg
    13381 gtatgaccgg acctcagcgc gtgtgtgcat cagtaagtta ccaacagatg cacggtttca
    13441 gcgcagaaca actgcgtaat accactcatc tggtgtgggc gacgggaggt ggaatggtgc
    13501 cggaagaaga gatgaatcaa tatctggcaa aaggccgtta ataacgtttc aacgcagcat
    13561 cgccatcctt tccctgggtg agcgatgctg ccgatggcgc agacttaaga tccccggtct
    13621 tacccgctat aaccccctgc ttttaccatc acccaacgcc tttcgctgta aacggcggtt
    13681 tcgcaaacca aaccaaaacc gtaagtaaga taaaagcaat agccgccata cggaaaatct
    13741 catttgcaga aatagaaagt gactgctggg ttatttcatt attaatttca ttaagaactc
    13801 ctgaaagcga accatagtat ttatccataa tttgtgacga tgaattaaac acggggttaa
    13861 attgatcgat ggttgctgtc aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg
    13921 tcattgtcaa cgacgtacca acggatcctg acaaggtacg aaaaaaatta ctcatactcg
    13981 aggcattggc aaatttatta tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa
    14041 agaaacaggc aacggcgaat ccctgaaaaa actgcggcaa aatgatacct gtaaaatcaa
    14101 tcgttggcat aaatgtcaca gaacgccagt aatagcaaac cgcatacatc aaaaaactaa
    14161 atgtcaccaa cacccgcatg tcaattttgt tgccataacg tcctatcaaa ggcgaaatca
    14221 atagtggcat gatgccgatg ggcgcataag caagtccggc ccatatcgca ttatacccca
    14281 tcgtttcctg gagtaactgc ggcataagga ctatcgctcc agagtaaaat aaatacgcgc
    14341 aggtgatact cacaatacca atggtgaaat tacgggactt aaacaaactg aggtcaagaa
    14401 tcgggttctc tgaggtcgac tcccaaatga ctaaagagat cagagaaata actgatacta
    14461 ctgttaatat tattattgta ctcgagttga accaatccag atcgcgccct ttatcaagca
    14521 taatttgcaa gccaccaaca ccgagcacta acagggtcag tcctggtaga ttcattttga
    14581 ccggtgaagt ctcagtttct cttcctttaa gtaaggttaa gcataatgtc aggacgataa
    14641 tccccatagg gacattgatt aaaaatatcc aaccccagct aaagttatcg caaatatatc
    14701 cgcctaatat cggcccacat atcggagcga taatcacggt cattgaccat aatgccagag
    14761 caaatgttct tttttctggt ggataattcc ttaataataa actctgtgac agtggaatta
    14821 acggccctgc cattaacccc tgaacgactc taaaaaatat cagcacatca agattggtcg
    14881 ataaactaca cattaatgaa gacagcgaaa aaaaagtgac tgaaagtaaa aataacctta
    14941 attcgcctat tctttgtgcc agcctgccgg taacagggat cgcaatggca tttgctacgc
    15001 caaacgaggt gataacccag gtgccttcgt ctgttgatgc tcctagaaag ccagatattg
    15061 tcggtattgc gacgttagaa atagtggaat ccaacatttg cataaacgtc gctaatgaca
    15121 atgcaatagt gacgcaccat aacgtcccac cggttaatgg tgccggagtt gatttagtga
    15181 ttgccattaa agttgtccat tatgcgaaat aatgttgcta atttcttttt ctatcggact
    15241 ggtatcgata actaaagcct tactggtata agccggcatg gacgtcacgg ttgaagccag
    15301 ctcaggcatc tcagcaatgt cttcgttctt cgtatcaata gttgcagtca tcgataaacc
    15361 aatgcgcaag gggtgttcca tgagttcttt tggatcaaga gaaacttcaa ccggtacacg
    15421 ctgaacgatt ttgatccagt tccctgtcgc attttgtgca ggtaataagg agaacgcatt
    15481 gccggttccc atattgatcc ctgtcacccg gccatgaaac acaacattct caccataaag
    15541 atcgctgata atattgaccg attgaccaat ccgtacatcc gtgagttgtg tttctttaaa
    15601 gttggcatta acccacattt gacgtgctgg tacgacagcc attaacgatt gtccgggact
    15661 cactgtttcg ccgacctgaa cacttcgctg ggcaatatag ccggtaaccg gactcttaat
    15721 atccgtacgt ttaagcgcca accaggcttc tttggttgca tccgcagctt caacgacttg
    15781 tggctgacgg tttaatggtg tgttcattac taaagcttta ttcgctttat aagcctggat
    15841 agcggcattc aatgccgctt tgctacttat taacgtatct ttggtatgct ccagcgtttc
    15901 ttttgaaata accccctgct tcgctaaagg cacacgacgg ttataatctt ctaaagattg
    15961 ttgatactga atacgtgctg aagcgacttc ggcactgtat tgtttatcct gtaagtacag
    16021 tttattcgtt tgccgaacaa tatttgccag attattttta gctttattga gtgcgatagt
    16081 ggcatcagtt ttatccagtg aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt
    16141 atgattaact acagtgacac tacctgagac ttgtgcagaa attggatctg cattcccc
//
LOCUS       DAZKFU020000096        16114 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZKFU020000096 DAZKFU020000000
VERSION     DAZKFU020000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.097"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..1524
                     /gene="puuA"
                     /locus_tag="ACHOQQ_RS12410"
                     /old_locus_tag="pgaptmp_002482"
     CDS             106..1524
                     /gene="puuA"
                     /locus_tag="ACHOQQ_RS12410"
                     /old_locus_tag="pgaptmp_002482"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGU4470719.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQDGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            1673..1837
                     /locus_tag="ACHOQQ_RS12415"
                     /old_locus_tag="pgaptmp_002483"
     CDS             1673..1837
                     /locus_tag="ACHOQQ_RS12415"
                     /old_locus_tag="pgaptmp_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470720.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            1827..3212
                     /gene="puuP"
                     /locus_tag="ACHOQQ_RS12420"
                     /old_locus_tag="pgaptmp_002484"
     CDS             1827..3212
                     /gene="puuP"
                     /locus_tag="ACHOQQ_RS12420"
                     /old_locus_tag="pgaptmp_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGU4470721.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            3346..3591
                     /gene="ymjA"
                     /locus_tag="ACHOQQ_RS12425"
                     /old_locus_tag="pgaptmp_002485"
     CDS             3346..3591
                     /gene="ymjA"
                     /locus_tag="ACHOQQ_RS12425"
                     /old_locus_tag="pgaptmp_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HGU4470722.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            3904..5547
                     /gene="sapA"
                     /locus_tag="ACHOQQ_RS12430"
                     /old_locus_tag="pgaptmp_002486"
     CDS             3904..5547
                     /gene="sapA"
                     /locus_tag="ACHOQQ_RS12430"
                     /old_locus_tag="pgaptmp_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGU4470723.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            5544..6509
                     /gene="sapB"
                     /locus_tag="ACHOQQ_RS12435"
                     /old_locus_tag="pgaptmp_002487"
     CDS             5544..6509
                     /gene="sapB"
                     /locus_tag="ACHOQQ_RS12435"
                     /old_locus_tag="pgaptmp_002487"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGU4470724.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            6496..7386
                     /gene="sapC"
                     /locus_tag="ACHOQQ_RS12440"
                     /old_locus_tag="pgaptmp_002488"
     CDS             6496..7386
                     /gene="sapC"
                     /locus_tag="ACHOQQ_RS12440"
                     /old_locus_tag="pgaptmp_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGU4470725.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            7386..8378
                     /gene="sapD"
                     /locus_tag="ACHOQQ_RS12445"
                     /old_locus_tag="pgaptmp_002489"
     CDS             7386..8378
                     /gene="sapD"
                     /locus_tag="ACHOQQ_RS12445"
                     /old_locus_tag="pgaptmp_002489"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGU4470726.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            8380..9186
                     /gene="sapF"
                     /locus_tag="ACHOQQ_RS12450"
                     /old_locus_tag="pgaptmp_002490"
     CDS             8380..9186
                     /gene="sapF"
                     /locus_tag="ACHOQQ_RS12450"
                     /old_locus_tag="pgaptmp_002490"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGU4470727.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(9237..10400)
                     /locus_tag="ACHOQQ_RS12455"
                     /old_locus_tag="pgaptmp_002491"
     CDS             complement(9237..10400)
                     /locus_tag="ACHOQQ_RS12455"
                     /old_locus_tag="pgaptmp_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HGU4470728.1"
                     /translation="MARVSPSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(10409..11782)
                     /locus_tag="ACHOQQ_RS12460"
                     /old_locus_tag="pgaptmp_002492"
     CDS             complement(10409..11782)
                     /locus_tag="ACHOQQ_RS12460"
                     /old_locus_tag="pgaptmp_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HGU4470729.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(11786..14893)
                     /locus_tag="ACHOQQ_RS12465"
                     /old_locus_tag="pgaptmp_002493"
     CDS             complement(11786..14893)
                     /locus_tag="ACHOQQ_RS12465"
                     /old_locus_tag="pgaptmp_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HGU4470730.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYLVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     SRRQE"
     gene            complement(14893..16014)
                     /locus_tag="ACHOQQ_RS12470"
                     /old_locus_tag="pgaptmp_002494"
     CDS             complement(14893..16014)
                     /locus_tag="ACHOQQ_RS12470"
                     /old_locus_tag="pgaptmp_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU4470731.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VTELTGRTSAAFSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
BASE COUNT         3796 a         4273 c         4109 g         3936 t
ORIGIN      
        1 caatttaaca tttgacaaac atttagtttg catacagatt cgaatggtgg tcattatatt
       61 ttacgctttg ataacgagcg gaaaacaaac caaaggcgaa gaatcatgga aaccaatatc
      121 gttgaagtag agaactttgt tcagcagtca gaagagaggc ggggtagcgc ctttacgcag
      181 gaagtgaaac gctacctgga gcgctacccg aatacgcaat atgttgatgt tttacttacc
      241 gatttgaatg gttgcttccg tggaaaacgt attcctgttt caagcctgaa gaagctcgag
      301 aaagggtgtt atttcccggc ctcggtattt gcaatggata ttctgggcaa cgtagtagaa
      361 gaagcaggtc tgggtcagga aatgggcgag ccggatcgta cctgtgttcc tgttctcggt
      421 tccttaaccc cttctgccgc cgatccagag tttatcggtc agatgctcct gaccatggtc
      481 gatgaagatg gcgctccctt tgacgttgag ccgcggaacg ttcttaaccg cctgtggcag
      541 cagctgcgcc agcgcggatt gttcccggtc gtagcggtag agctggagtt ctatttactg
      601 gatcgccagc gcgacgctga agggtatctg caaccgccct gcgcgcccgg cactgatgac
      661 cgcaatacac aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc
      721 aatgatattg atgaactggc gcagttacag ctgattccgg cagatggcgc ggtcgctgag
      781 gcctcgccgg gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc
      841 gatgatgcgc tggcactaaa acgcctggtg cgtctgatgg cagaaaagca taagatgcac
      901 gccactttta tggcgaagcc gtatgaagag cacgcgggca gcggaatgca tatccatatc
      961 agtatgcaaa ataatcgtgg cgagaacgtg ctttctgaca cagaaggcga agattcgccg
     1021 ctgctgaaaa agatgctcgc cggaatgatc gatctgatgc cgtcatcaat ggcgttgttg
     1081 gcaccaaacg tgaactcgta tcgccgcttc cagccgggga tgtatgtacc gacgcaggcg
     1141 tcgtggggcc ataataaccg taccgtcgcc ctgcgtattc catgcggcga tcgtcataat
     1201 caccgcgtgg aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg
     1261 atttttgccg gtattttgca cggtcttgat aacgagctgc cgttgcagga agaagtcgaa
     1321 ggcaacgggc tggaacagga tggcttaccc ttcccgattc gccagagcga tgccctgggt
     1381 gagtttatcg agaatgatca cctgcgccgc tatttaggcg aacggttttg ccatgtgtat
     1441 cacgcctgta aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt
     1501 gagtggatgt tgaaaaacgc atagtacccc ttgcggggtt gtttcatcgg cctgagcgga
     1561 gccaggcctg acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca
     1621 ggcggcaacg ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat
     1681 gattaaatcc ccgcatggcg agggaggctg cgtatgcgcc cctcctgcga ctgactggac
     1741 tccgccccct ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac
     1801 cttattacgc aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc
     1861 aacccggcaa aacccgtctg cgaaaatcac tgaaattgtg gcaggtggtg atgatgggtc
     1921 tggcctatct cacgccgatg actgtgttcg atacctttgg cattgtatct ggcattagcg
     1981 acggtcacgt cccggcatcc tatttgctgg cgctggcggg cgtgctgttt accgctatca
     2041 gctacggcaa actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa
     2101 agtcgattaa tccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct
     2161 ttttgccgat gatcaacgta ctgttggcaa aaatctatct ctccgccctc ttcccggaag
     2221 tgccgccgtg ggtgtgggtg gtgaccttcg tcgccatttt aactgccgcg aacctgaaga
     2281 gcgttaacct ggtcgccaac ttcaataccc tgtttgtgct ggtgcaaatc tccatcatgg
     2341 tggtgtttat cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct
     2401 ggtcacttca accgtttatc agcgagaacg cgcacctgat cccgattatt accggggcga
     2461 cgattgtgtg cttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc
     2521 cggacgccgc acgcgtgatc ccgaaagcca tcttcctgac ggcggtctac ggtggcgtta
     2581 tctttatcgc ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag
     2641 acccggacgc tgcactgcct gaaattgcgc tgtacgtcgg cggcaagctg ttccagtcta
     2701 ttttcctctg caccacattt gtgaacacat tggcctctgg cctggcttca catgccagcg
     2761 tgtcgcgcct gctgtatgta atggggcgtg acaatgtgtt cccagaacgt gtgtttggct
     2821 atgtacaccc gaaatggcgt accccggctc tgaacgtcat tatggtcggt attgtcgcga
     2881 tgtcggcatt gttctttgat ttagtcaccg cgacagcatt gattaacttc ggtgctctgg
     2941 tggcgtttac cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga
     3001 ataaaagctg gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg
     3061 tgggtgtgct gtgggttaac ctcgagtcaa cgtcactgac actcggtctg gtatgggctt
     3121 cgttaggcgg cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgttgtatg
     3181 atggtgacag aacgccagtg agcgaaacgt aatagtcgga tgcgcggcgc attatcctgc
     3241 ctgagggtaa tgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc
     3301 tatcatccgc ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc
     3361 ccgctaaaat attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca
     3421 gacgctgaac gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac
     3481 aatgaagaga tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc
     3541 gtacgcattg atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat
     3601 tgcagacgat cggtcagtat cgttgtctgg ataaagcgtt cacgccgcat ccggctaaca
     3661 atgacacgca ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg
     3721 ctaagttgtt ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg
     3781 gcgacgccac ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac
     3841 ggtaatggca aatgtcatta aatattagta agatagcttc tttacataaa atcccttaat
     3901 attatgcgcc aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca
     3961 atcgccgcgc ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc
     4021 gtcagcgggc aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat
     4081 acccttgccg cccagtttta tgatcgactg ctggatgttg atccctatac ctatcgcctg
     4141 atgccagaac ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac
     4201 ctacgtcgcg atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat
     4261 gccgacgatg tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac
     4321 gtcaacggca gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc
     4381 gtccgtaaac tggataatca taccgttgag ttccgtctgg ctcagccgga tgcttctttt
     4441 ttgtggcacc tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta
     4501 gagaaagaag atcgccagga gcaactcgac cgtcaaccgg tcggcactgg accgtatcag
     4561 ttgtcggaat accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc
     4621 ggtaaaccgt taatgccgca ggtggtggtg gatttaggct ccggcggcac cggacgtctg
     4681 tcgaaactcc tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc
     4741 attttgcgtg acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat
     4801 ctggcattta acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcgctggca
     4861 ctggcgatta ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg
     4921 gcctctattt taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac
     4981 aatccggcga aatcgcgcga acagttgaag tcgttggggc tggaaaattt aacgctgaaa
     5041 ctgtgggtgc ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg
     5101 attcaggcgg atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc
     5161 tttcaggagg cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg
     5221 gacagtaacg acccggacag tttcttccgt ccgttactga gctgcgcggc aattcattca
     5281 cagaccaacc tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc
     5341 tcctcgcagc agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg
     5401 caggaattgc ccattttgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat
     5461 atcaaaggtc tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag
     5521 aaacaggatg aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga
     5581 ttgtcacctt gttcctgctg accttcgttg gcttcagctt gagctatttc actccacacg
     5641 cgccgttaca aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc
     5701 actgggattt tggtgtttcc agcattaacg gtcaaccaat tgccgaacag ttaaaagagg
     5761 tcttcccggc gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca
     5821 tccccgtggg gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg
     5881 ccatcgcctt actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt
     5941 tttgttcact cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag
     6001 tgaaaccgat taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg
     6061 atgaaatgat catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg
     6121 cgccaacaac tgaagtgatc cgcctgatgc gtatcagcac tattgaagtg tatgaccaga
     6181 actatgttaa agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg
     6241 ttctgcataa cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc
     6301 tgacgctggc gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa
     6361 ttaacgccat ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct
     6421 cactggtcat tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga
     6481 aacataagga atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg
     6541 ctgcgtaccg cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc
     6601 tgcgcggggc tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac
     6661 cagcaatttc tcggttacca attattgccg ccgtcatggt cgcgctatgg cgaagtttct
     6721 ttcttcctgg ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct
     6781 gcgccaaccg tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta
     6841 ctgggcacct tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg
     6901 gataccttac tggcgatccc ttccctgctg ttggcaatta tcgttgtagc gtttgccgga
     6961 ccgagtttgt cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt
     7021 tcgatttaca gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg
     7081 gatggcgcat caacgctgaa tattctctgg tttgctgtga tgcctaacat caccgccggg
     7141 ctggtcaccg agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc
     7201 tttctcgatc tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg
     7261 ctggaactga tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt
     7321 agcgtgctgt tggttaacct ccttggcgac ggtgtccgtc gtgcaattat tgcgggggtg
     7381 gaataatgcc attactcgat attcgtaacc tgactattga atttaaaacc ggggatgagt
     7441 gggttaaagc cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg
     7501 ttggtgaatc cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag
     7561 ataactggcg tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct
     7621 ccgcacgcga acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc
     7681 agtcgtgtct tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct
     7741 ggacctataa aggccgttgg tggcagcgtt ttggctggcg caaacggcgt gccattgaac
     7801 tgctgcaccg cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt
     7861 tgaccgaagg tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc
     7921 tgctgattgc tgacgaaccg acaaactcaa tggagccaac aacccaggcg caaatctttc
     7981 gcctgctgac gcgtctaaac caaaacagca ataccactat tttgcttatc agccatgact
     8041 tacaaatgct tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacggtgg
     8101 aaaccgcgcc gagtaaggag ttagtgacga tgccacatca tccttatacc caggcgctga
     8161 tccgcgcgat accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctac
     8221 ccggcgctat cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc
     8281 catatgcaca acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct
     8341 atgcctgtca tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa
     8401 gtgcgtaatt taagtaaaac gttccgctac cggaccggtt ggtttcgtcg ccagaccgta
     8461 gaagcggtaa aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc
     8521 gagaatggtt cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agaacctacc
     8581 agcggcgagt tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt
     8641 cagcgtattc gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc
     8701 tcgcaaattc tggattttcc actgcgcctg aacaccaacc tggaaccgga acagcgtcgc
     8761 aaacagatta ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat
     8821 ccgcatatgt tggcccccgg gcaaaaacag cgtctgggtc tggcgcgcgc gctgatattg
     8881 cgcccaaaag tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg
     8941 cagttgatta atctgatgct ggaattacag gaaaaacagg gcatttcgta tatttatgtt
     9001 acccagcata tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc
     9061 gaggttgtcg agcgaggcag caccgcggat gtgctggcat caccgctgca tgaactcacc
     9121 aaacggctga ttgccggtca ttttggcgag gcattgacgg cggatgcgtg gcgtaaggac
     9181 cgttagtgtt ctgcacctca tcctgacagg ggccccccct caggatgagg tattcattac
     9241 ttttgattgt cgattctggt gagagcaacc aggaatgcca gtgtataaca caccgtaatt
     9301 gccagactca tttttaacag tgtttctccg ccaaatcccg acaatggcgc ggctataccg
     9361 ccgaagacaa acatgctcat ccccatcaac gcagacgccg ttccagattc ctgtgtccct
     9421 actgcactca tagcctctgc cccgccaaca gtgccgatgc cgctacaaaa tgcaatagtg
     9481 aaaaataatg ccaccagtgc cggaatgggc aattgtgtcc atgcgcataa gaccgtgagc
     9541 aatgcacaca aaattgccgc tatcaggcca cctcgcaaca atgtcatcgc attaatccgt
     9601 cgcgccagac gcgaaaagat ccatgaagca atgataagtc cgatgccgtt aaggccaaac
     9661 accaggctaa attgcattgg actaaagcca aattccttct gcaacacgaa cgacgaagag
     9721 ccgatatacg caaacaagcc tgccagcata aaactttgaa tcaggcaaaa gcgcataaaa
     9781 cggcggttct gcaccacgct tcgtccggtt aatagcaatg atgagcccct tttattttct
     9841 ggcaaggtct cattaataaa taacacgcag cccagcaaca atacggtgct aatttcagcc
     9901 attacccaga ataaagtgcg ccagtcaaaa gtgctaacaa tgtacccgcc cagcactggc
     9961 gacaacaccg gtgccaggcc attcaccgtc attaacagcg caaaaaactg ggtcaacgtt
    10021 actccctgat atttgtcacg agcaatagaa cgagagagca ccgaaccacc cgcccccgcg
    10081 atcccttgaa taaaacgcca gaccaccagc cagtaaatat tgttcgttgt cgcgcacaaa
    10141 atggaagaaa caataaacaa tagcaacgac aagattaacg gacgtttacg ccctattttg
    10201 tcgctcagag ggccaaataa cagttgtccg acgccaagac cgatcagtga tgcagtcagc
    10261 gttaattgcg ttatggtcgt ggttgccgcc agttgctcag acatctccgg caaagccggc
    10321 aaataaagat cggtacacat cgggccgata ccggctaaaa gaccaagaat caatgcccat
    10381 gaaggagaga ctctagccat attattcctt acttgagtga tgacatgccg ccgcccagcg
    10441 actgccagag cgtgattcgg tttgtaaaat cagtcagttg cagtgccagc agctcctgct
    10501 gtgcgctcca caagctgcgc tgcgccgtca acacggtcag gtaatcacca acacccgtct
    10561 gatagcgacg caagccaaca tcgaccgttt gttgctcagc tttaacatac tggcgctgtg
    10621 catccagttg ctcttcaagc gtggtccgcc gcgccagtgc gttagcgaca tctttaaatg
    10681 cgctctgaac ggttttttca taggtggcaa tgagtccacg cttttgtgct tctgcgtaac
    10741 gaagctgcgc cagattactt ccgccagtga acagcggtaa tgtgaccgat ggcgtaaatg
    10801 accagatctg cattccgtgg ctgaacaacg acgacaatgc gtcactgcca acaccagcgc
    10861 tggcagtcag cgaaatcgtc gggaaaaagt tagcccgcgc cgcgccaata tcggcattgg
    10921 cgctcttcaa attatgttcc gcttcctgga tatccgggcg acgcagcaga acgtcagatg
    10981 acactcccgc aggaaccaga cttatcatct gttccggcag gctttccagt gttcccggta
    11041 gcaaattttc ctcaagcgtt gtacctgcca gtaagttcag cgcattttta tcctgcatca
    11101 cttgtgtctg atagctggca acgctggcgc gcgcttgctg ataaacactc atcgcctcac
    11161 tgacatctgt cgctgccgcc gtgccaacct gttgctggcg ctggataatt ttcaatgagt
    11221 tttctgcact ggtcatcgtt tcttttgcca gggccaggtt gctgttatcg gccgccagtg
    11281 tcagccaggc ggtgctgatt tcagcaatca gcgttaaacg ggtgttctgg gctgtaaatt
    11341 cactggcaag ccaggtttct cgcgccgccc gagataaact ctgattacga ccaaacagat
    11401 ctaacgtaaa actggacacc gtgccgtcag cctcggcagt ggtttccgta ccattcgcca
    11461 gcgaacgact gcgggttgaa cttaaggccg cattcaccgt cgggaataac gacgcattag
    11521 tttgcccata gagcgcccgt gctgagtcaa tatcagcaat cgctttttgc acatcgcggt
    11581 tactgttcag tgcaatagtc accacctgct gcaatcgggg atcgtggatc acctgctgcc
    11641 agtcatggct gattgccttt ccctggccct gtgctcccgg caaggtcgcc gggattgggg
    11701 attcaggcgt gctgtagtgg ggatcgagcg agacacatcc cgcactcaac agcaccagaa
    11761 aaataagaga acgacgcaac ataacttact cctgacggcg cgatttaccg gcaaacaaac
    11821 gtttcaccag tacaaaaaac agaggaacaa agaaaatagc cagcaacgta gcggtcagcg
    11881 taccgccaat aatgcccgta ccaatggcga tgcggctgtt cgcccctgcg ccggttgcta
    11941 tcgccagcgg cattaccccc gcaataaacg ccagcgaggt catgatgatt gggcgtaaac
    12001 gagtctgcgc cgcgcgtaat gccgcacgac tcaaggaata accctctgca accgccgctt
    12061 cagcaaattc cacaatcaga atggcgtttt tcgacgacaa gccgatagtg gttaatagcg
    12121 ccacctggaa gtaaacatcg ttgtttaaat cacgcatcca ggccgccagc gccgcgccaa
    12181 gaagccccag agggatcacc agaatgaccg agaacgggac tgaccagctt tcgtacagtg
    12241 ctgcaaggca gaggaaaacc accagaatag aaactgcata cagactcaat gcctgaccgc
    12301 tggccagttt ctcctgcaat gacaaaccac tccaggccca ggttgttccc gcaggaagac
    12361 tgtttgccag ttcctccatt tttgtcattg catcgccgga actaaagcca gtcgcatttt
    12421 ccccctgaat ttcataggct gcagaaccgt tgtagcgcac cagtctttcc ggtccataca
    12481 gccagcgggt ggtcgcaaaa gcagagaatg gtgtcatggc gttatcacta ccgcgcacaa
    12541 accatttacc taagtctgac ggcgcggaac gaaattcact gtcgccctga atgtagactt
    12601 ttttcacacg accacggtca ataaagtcat tcacataagt accgccccac gcactggaca
    12661 gggtgtcggt gacatcattc aaactcaacc ctaatgacac cgctttatta ctatcaatat
    12721 ctacctgcaa ttgcggcatt tgtggcaaat cattggcacg aacagaatgt aactccggac
    12781 tttgattcgc tttttctatc aactgattac gcatttgcag tagtgtttcg cgatcggttc
    12841 caccgttagc taacaattca aacgtaaaac cattgctttg ccccagccca tcaacggctg
    12901 gcggcgtcat cgcaaatacc gtggcatcac gaattgtgcc cagctctttg gttgcccgta
    12961 gggcgatagc ctgggcggtg ttttctgccc ctttacgttg agaccagttt ttcaacgaaa
    13021 caaacgccat cccggtgttc tgtccgctgc cgctaaaact gaaaccatca acggtaaaaa
    13081 tgacatcggt atttgctttc tcgttaatca ggaaccagtc aacaatctgg cgattcactt
    13141 ctgctgtacg ggcagcggtc gccccggccg gcagcgtgta ctgcaccatg atttcgccct
    13201 ggtcttcagt gggtaagaaa ctgcccggca gtttccacat catcagggcc attccaccac
    13261 ccagtacgag ataaagcccc atcgttcgag cagcacggcg caggacataa attacgcctc
    13321 gctgatattt atcttcagta cggcggtaaa agcggttaaa tgcgccgaaa aagccttttt
    13381 tatgtggcgg aacatgctgg aggacggaac cgcacagggc gggagtcaag gtcaatgcca
    13441 ccaccacgga aagcagcatt gcggagataa tggttatcga aaactgacga taaattaccc
    13501 ccgtagatcc cccaaagaag gccataggca ggaatacggc tgacaacacc agcgcaatgg
    13561 caaccagtgc gccagaaatc tcgcccattg atttttccgt ggcttcacgc gcgggcaacc
    13621 cttcgtcacg cataatgcgt tcgacgttct ccactaccac aatggcatcg tccaccagca
    13681 agccgatcgc cagcaccatt gcaaacaacg tcagggtatt aatcgaatag ccaaacaacg
    13741 caagaacgcc gaatgtgccc agcaaaacga cggggacagc taacgccgga atcagtgtgg
    13801 cacgcaggtt ttgcaggaat aaatacatca cgcaaaccac caatacgatg gcttcaaaca
    13861 gcgtctgaat tacgtcctct acagagattt taataaattc agtgctgtct ttcgggtaag
    13921 cgatgtcgta tccctgcggc atgttccgct ggaattcggc aatcttatcc ttgaccagcg
    13981 tcgccgtatt cagcgcattc gcaccgggcg aaagcattac agcaaccccg gcagacgggt
    14041 gaccgttaag tttgccgata gcggtataat cttcactgcc catctcaacg cgagccacat
    14101 cttttatacg aacaactgcg ccgtctgact ggcttttcac gataatattt ttgaactgat
    14161 ccaccgtctg caagcgagac tgcgcacgta ccgttgcggt cagttgctga gtattcggtg
    14221 aaggcaatgc ccctattttc ccggcagtaa cctgcacgtt ttgcgcttca atagcacttt
    14281 gcacgtctga aggcattagc gagtaagacg ccagtttggc cgggtcaagc cagatgcgca
    14341 tagcgtattc cgcgccaaag acttgcagac tccccacacc ttcaacacgc gccagcgggt
    14401 cctgaacgtt actaaccaac cagtcggcga tatccgaact ggaggctttg tcagtggtgt
    14461 catacacggc agcaatcagc aaaaagttac tttgtgattt ctccaccgta acacccgttt
    14521 gctgcacttc gctgggtagg cgcgactccg cctgctgaat tttattctgc acctgaacct
    14581 gtgcagtatc tggatcggta ccttgttcaa aggtcacatt aatactgacc gaaccatcag
    14641 agctgctggt tgagctgaaa tagagtaaat tatcaagccc agtgagttgt tgctcgataa
    14701 cctgagtcac gctgttctcc agcgtttcgg cagaagcacc agtataagtg gctgaaattt
    14761 taatggtcgg tggcgcaacg tcaggatatt gcgcgacggg caatgtgcga atagccagaa
    14821 tccccgccaa cataatcaaa atggcgataa cccaggcaaa gaccgggcgg cgaacaaaaa
    14881 agcgcgaaaa catcaggcgt tgcctccatt agcctgaact tcaacagcct tgaccgtctg
    14941 ccctgaagtg acttttgcag aaccttcaac aatcagtcgg tcgccgttgt gcaggccgtt
    15001 tagcaccagc catttatcac catatgtttc tcccgtttcg agcgttcgct gctctacttt
    15061 attgtcttta ttcaccacca gcgcagttgc attgccttta gcatcgcgcg tgacgccctg
    15121 ttgcggcgcg agaatagcgt cttccatcac gccttcatcg acacgagcgc gaacaaacat
    15181 tcccggtaat aactgctgtt gtggattggg gaaaatcgcc cgtaatgtca ccgaaccggt
    15241 agactcatct acagcgactt cggtgagttc cagacgccct ttttcgctgt aggttgtgcc
    15301 atcttccaga ataagtgaga cgctcatggt gtcactgttg gtcgccagtg actgtttacg
    15361 cagacgtaat aaatcgacac tggagcgagt gaggtcgaca tacattgtat ccagaccacg
    15421 aatagtcgtt aacgctgtat cttgcgacgc ggtcaccagt gcgccagggg ttaccgacga
    15481 aatgccaatg cgccctgaaa tcggtgcggt taccgtggtc cagtcaagat taatgcgcgc
    15541 agtttccagt gcggcttttt tcgcctctac actggcttta tcttgtgcac aggtagactg
    15601 agcatcatca gcatcctgtt gtgaaacacc gttctctttc accagtcggg cataacgctg
    15661 cgctttctgg caatcggctt ttaccagtgc ctgtgcttgt tgtaatgctg cccgagcttc
    15721 attccatgca gcctgataac tggccgcatc aatctggtag agcggctgtc cagccttgac
    15781 cagatcacct tccttaaata agcgtttctg gataattccc ccaacctgcg gacgtacttc
    15841 ggcactgaac gcagcactgg tgcgtccggt taattcagtg actaccgaga ccggttgaga
    15901 tttgactgta acaacgccga cttctgtttc tgacggggat gaattgttac tttgcgtatt
    15961 atcacaacca gataagagca gcattgccac tacagatgtt gctatatact tcattatttt
    16021 ctccagggtg agaaataccc tcctcattaa aattaatgag gatatgtcag ggatattcag
    16081 ttataacgaa cccgcatcgc gaaatgaatt tcgc
//
LOCUS       DAZKFU020000097        16104 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZKFU020000097 DAZKFU020000000
VERSION     DAZKFU020000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.098"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..1765
                     /locus_tag="ACHOQQ_RS12475"
                     /old_locus_tag="pgaptmp_002495"
     CDS             80..1765
                     /locus_tag="ACHOQQ_RS12475"
                     /old_locus_tag="pgaptmp_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGU4470732.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(1940..2476)
                     /gene="rcdA"
                     /locus_tag="ACHOQQ_RS12480"
                     /old_locus_tag="pgaptmp_002496"
     CDS             complement(1940..2476)
                     /gene="rcdA"
                     /locus_tag="ACHOQQ_RS12480"
                     /old_locus_tag="pgaptmp_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGU4470733.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVIDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            2560..3768
                     /locus_tag="ACHOQQ_RS12485"
                     /old_locus_tag="pgaptmp_002497"
     CDS             2560..3768
                     /locus_tag="ACHOQQ_RS12485"
                     /old_locus_tag="pgaptmp_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4470734.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            3768..4583
                     /gene="ybjI"
                     /locus_tag="ACHOQQ_RS12490"
                     /old_locus_tag="pgaptmp_002498"
     CDS             3768..4583
                     /gene="ybjI"
                     /locus_tag="ACHOQQ_RS12490"
                     /old_locus_tag="pgaptmp_002498"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGU4470735.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQDIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            4669..4953
                     /gene="ybjH"
                     /locus_tag="ACHOQQ_RS12495"
                     /old_locus_tag="pgaptmp_002499"
     CDS             4669..4953
                     /gene="ybjH"
                     /locus_tag="ACHOQQ_RS12495"
                     /old_locus_tag="pgaptmp_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGU4470736.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(4994..6226)
                     /gene="mdfA"
                     /locus_tag="ACHOQQ_RS12500"
                     /old_locus_tag="pgaptmp_002500"
     CDS             complement(4994..6226)
                     /gene="mdfA"
                     /locus_tag="ACHOQQ_RS12500"
                     /old_locus_tag="pgaptmp_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGU4470737.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            6511..7107
                     /gene="ybjG"
                     /locus_tag="ACHOQQ_RS12505"
                     /old_locus_tag="pgaptmp_002501"
     CDS             6511..7107
                     /gene="ybjG"
                     /locus_tag="ACHOQQ_RS12505"
                     /old_locus_tag="pgaptmp_002501"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4470738.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQATGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7165..7923
                     /gene="deoR"
                     /locus_tag="ACHOQQ_RS12510"
                     /old_locus_tag="pgaptmp_002502"
     CDS             7165..7923
                     /gene="deoR"
                     /locus_tag="ACHOQQ_RS12510"
                     /old_locus_tag="pgaptmp_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGU4470739.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(7970..9172)
                     /gene="dacC"
                     /locus_tag="ACHOQQ_RS12515"
                     /old_locus_tag="pgaptmp_002503"
     CDS             complement(7970..9172)
                     /gene="dacC"
                     /locus_tag="ACHOQQ_RS12515"
                     /old_locus_tag="pgaptmp_002503"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4470740.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            9419..10045
                     /gene="gstB"
                     /locus_tag="ACHOQQ_RS12520"
                     /old_locus_tag="pgaptmp_002504"
     CDS             9419..10045
                     /gene="gstB"
                     /locus_tag="ACHOQQ_RS12520"
                     /old_locus_tag="pgaptmp_002504"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGU4470741.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDTLFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(10042..11157)
                     /gene="yliI"
                     /locus_tag="ACHOQQ_RS12525"
                     /old_locus_tag="pgaptmp_002505"
     CDS             complement(10042..11157)
                     /gene="yliI"
                     /locus_tag="ACHOQQ_RS12525"
                     /old_locus_tag="pgaptmp_002505"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGU4470742.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(11268..11651)
                     /gene="bssR"
                     /locus_tag="ACHOQQ_RS12530"
                     /old_locus_tag="pgaptmp_002506"
     CDS             complement(11268..11651)
                     /gene="bssR"
                     /locus_tag="ACHOQQ_RS12530"
                     /old_locus_tag="pgaptmp_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGU4470743.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            11864..13189
                     /gene="rimO"
                     /locus_tag="ACHOQQ_RS12535"
                     /old_locus_tag="pgaptmp_002507"
     CDS             11864..13189
                     /gene="rimO"
                     /locus_tag="ACHOQQ_RS12535"
                     /old_locus_tag="pgaptmp_002507"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGU4470744.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(13236..14564)
                     /locus_tag="ACHOQQ_RS12540"
                     /old_locus_tag="pgaptmp_002508"
     CDS             complement(13236..14564)
                     /locus_tag="ACHOQQ_RS12540"
                     /old_locus_tag="pgaptmp_002508"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4470745.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(14572..>16104)
                     /locus_tag="ACHOQQ_RS12545"
                     /old_locus_tag="pgaptmp_002509"
     CDS             complement(14572..>16104)
                     /locus_tag="ACHOQQ_RS12545"
                     /old_locus_tag="pgaptmp_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HGU4470746.1"
                     /translation="YYPGGNLLNASDKLFYLPFAFIIIVLLVVYLLTTRVFRRQFSEM
                     TELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYD
                     QESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYA
                     QCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGEN
                     AWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQ
                     PIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFR
                     HFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERI
                     NEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKI
                     VRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLV
                     KE"
BASE COUNT         4006 a         4059 c         3874 g         4165 t
ORIGIN      
        1 ccctgaattc tggtaaaaaa cattatcgta aattaccatt tctttcaaca gcttactagt
       61 aaacaagaag ttagcctccg tgaatataaa cgtcgccgaa ttgttaaatg ggaattacat
      121 tctgttatta tttgtggtcc tcgcgcttgg gctatgtctc ggaaagttac gacttggttc
      181 gatccaactg ggtaattcca ttggcgtttt agtcgtatcg ctgttattag gccaacaaca
      241 tttcagcatt aacaccgatg cgcttaatct tggctttatg ctgtttattt tctgcgtcgg
      301 ggtcgaagcc ggaccgaact ttttttccat tttttttcgc gatgggaaaa attacctaat
      361 gttagcactg gtgatggttg gcagtgcgct ggtgatcgcc ttagggttag gtaagctgtt
      421 tggctgggat attggcctga cggccggtat gttagcaggc tctatgacgt cgacaccggt
      481 tctggtcggt gctggcgata cactgcgtca ttccggcatg gaaagcaggc agctctcact
      541 ggcactggat aatctgagcc tcgggtatgc cttaacctat ttaatcggtc tggtgagttt
      601 gattgttggt gcgcgttact tgccgaaatt gcagcatcag gacttacaga ccagcgccca
      661 gcaaatcgcc cgcgaacgtg gcctggacac tgatgccaac cgtaaggttt atttaccggt
      721 gatccgcgcc tatcgcgtcg gcccggagct ggtggcctgg accgacggca aaaatctgcg
      781 tgaactgggt atttatcgac aaaccggctg ctacattgaa cgtattcgac gtaacgggat
      841 tctggcaaat ccagacggtg atgccgtgct acaaatgggc gatgaaatag cgttggtagg
      901 ctatcccgac gcccatgccc gactcgatcc cagcttccgt aacggcaaag aagttttcga
      961 tcgtgacctt ctcgacatgc gtatcgtcac tgaagaagtg gtcgttaaaa accataacgc
     1021 cgttggcaaa cgtctcgcac aactgaagtt gaccgatcac ggttgcttcc ttaaccgcgt
     1081 tattcgtagc cagattgaga tgccgataga tgacaacgtc gtgcttaaca aaggtgacgt
     1141 tttacaagtc agcggcgatg cccgccgcgt aaaaaccatc gccgatcgca tcggctttat
     1201 ctcgattcac agccaggtca ctgacttgct ggcattctgc gccttctttg ttattgggct
     1261 gatgatcggg atgatcacat tccagttcag cacattcagt ttcggcatgg ggaacgctgc
     1321 cgggttgtta ttcgccggaa ttatgctggg ctttatgcgt gctaaccacc cgaccttcgg
     1381 ttacattccg cagggtgcat taagcatggt gaaagagttc ggcttaatgg tgtttatggc
     1441 aggcgttggt ctgagcgccg gtagcggtat taataacggt ctgggcgcga ttggcggtca
     1501 gatgttgatt gccggattga ttgtcagtct tgtgcccgtg gttatctgtt tcttgttcgg
     1561 tgcttatgta ttgcgaatga accgcgcact gttgttcggc gcaatgatgg gcgcacgcac
     1621 ctgcgcgccg gcaatggaga tcatcagtga tacagctcgc agtaacatcc cggcgctggg
     1681 ctatgcgggc acctacgcaa tcgccaacgt cctgctgacg ctggcaggga caatcatcgt
     1741 catggtatgg ccaggattag gataaaactg aagttgccct gaaaatgaaa tttttttgca
     1801 caaccgcaga acttttccgc agggcatcag tcttaattag tgccactgct tttctttgat
     1861 gtccccattt tgtggagccc atcaaccccg ccatttcggt tcaaggttgg tgggtttttt
     1921 gttgcctaaa atttatctac tacccagcaa ccctctcaac catcctcaaa atctcctcgc
     1981 gcgatagcgg cttacggtcg gtgacaaaat gcagcgtcat cccctcaata aacgcatcaa
     2041 gcgcgcgggc ggttccgggt tcaaaccatt gttcgagcgt ttgctgactg cgctgcatcc
     2101 agttttgcat taccgttttt aatagcggtt ttcggctagc cagcgcgtag agctggtaca
     2161 tcagctccat gttatccggc gttgccacct gtgagctgta gatcatatcg gtgatagcct
     2221 ggcatgcgcc ttgagcatca ataacatcgc taaaaaatgc ctgatattgc cgggacatga
     2281 tctcagtaaa actgctgaac gcctccagca acaactcatc aattcctgaa aaatagtagg
     2341 tcatcgatcc caacggtacc ccggcaaggg tagcaatttt gcggtgcgta acagcatgta
     2401 ttccgtaaag tttcaccgcc tccagcgtgg cctggataat tttttctcgc cgttgcggat
     2461 cgttagcgcg acgcatgtca tttcctctat ctgcaattgt gtacaaatgt acacaacctt
     2521 gctaatgttg tcttccctct tcttattttg acgtgatcta tgaccgtaaa ttcttcacgt
     2581 aatgcattga aacgccgaac ctgggcgctg tttatgttct tctttttgcc gggcctgtta
     2641 atggcgtcct gggcaacccg tacgcctgct atccgcgata ttctctctgt ctcgatcgct
     2701 gaaatgggcg gtgttctctt tggtctgtcg atcggttcga tgagcggtat tctctgctcg
     2761 gcgtggttag tgaaacgctt tgggacacgt aatgtcatcc tggtcaccat gtcctgcgca
     2821 ttggtcggga tgatgatatt aagtctggca ctctggctga catcgcccct gctctttgcc
     2881 gttggtctcg gcgtctttgg ggcaagtttt ggttctgcgg aagtggcgat aaacgttgaa
     2941 ggtgccgccg ttgagcgaga aatgaataaa acggttttgc cgatgatgca cggtttttat
     3001 agcctgggca cgctggcagg cgctggtgtc gggatggcac tgacggcctt tggcgttccg
     3061 gcaacggtgc acattttatt ggcggcgctg gtaggcatcg cgcctatata tatcgccatt
     3121 caggcaatcc ctgacggtac gggcaaaaat gctgccgatg gcacccagca tggcgaaaaa
     3181 ggcgtacctt tttatcgcga tatccagttg ctgctgattg gtgttgtggt gctggcgatg
     3241 gcctttgccg aaggttctgc caacgactgg ttacccttat taatggttga cggtcacggt
     3301 tttagcccca cttccggctc gctgatttat gccggtttta ccctggggat gaccgttgga
     3361 cgcttcactg gcggttggtt catcgaccgt tacagtcgcg ttgccgtggt tcgggccagt
     3421 gcgctaatgg gggcgttggg tattgggctg attatttttg tcgatagcgc ctgggtcgct
     3481 ggggtgtctg ttgtactctg gggactgggt gcctcgctgg gcttcccgct gaccatttct
     3541 gccgccagcg ataccggccc cgatgcacca acccgcgtca gtgtggtagc aacgaccggt
     3601 tatctggctt tcctcgtcgg gccgccgctg ctgggctatc tcggcgaaca ttatggatta
     3661 cgtagtgcaa tgctggttgt actggcgctg gttattctcg cggctattgt cgcgaaagcc
     3721 gtcgccaaac ccgataccaa aacgcagacg gcgatggaga atagttgatg agcattaaat
     3781 taattgcggt agacatggat ggtactttct taagcgatca aaaaacctat aaccgtgagc
     3841 ggtttatggc tcagtatcag caaatgaaag cacaagacat tcgctttgtg gtcgccagcg
     3901 ggaatcaata ttatcagttg atctctttct tccctgaaat tgctaatgaa attgcctttg
     3961 tggctgaaaa cggcggctgg gtagtgagtg aaggcaaaga tgtttttaat ggcgagctgt
     4021 cgaaggatgc gtttactact gtcgtggaac atttgctgac gcgcccggaa gtggaaatta
     4081 ttgcctgtgg aaaaaatagc gcctatacgc tcaaaaaata tgacgatgcc atgaaaacgg
     4141 tggcggaaat gtattatcac cgtctggaat acgtcgataa ctttgacaac ttagaagata
     4201 tcttctttaa gtttggcctg aatctttccg atgaactgat tccacaagta caaaaagcat
     4261 tacatgaggc catcggcgat attatggtgc cggtccacac cggcaacggc agcatcgatc
     4321 tgattatccc cggcgtacat aaagccaatg gccttcgcca actgcagaaa ttatggggaa
     4381 tagacgacag cgaagtggtg gtctttggcg atggcggtaa cgatattgag atgctgcgtc
     4441 aggcaggctt tagttttgca atggaaaatg ccggcaacgc ggtcgtcgca gcggcaaaat
     4501 accgggcagg ctccaataac cgtgaaggcg tactggatgt gatcgataaa gttcttaaac
     4561 acgaagcgcc atttgaccaa taaaaacggc agcacgcccg gcaacacaat acctgaatga
     4621 aggctgcata attcgcggaa attaacccgc tgcattattt caggcgaaat gtttatgaaa
     4681 aattgtctac tgttgggcgc gcttttaatg ggctttactg gtgtggcgat ggcgcaaagt
     4741 gtcaccgtcg atgtgccgag tggatacaaa gtggttgtgg ttcctgattc agtcagcgtt
     4801 ccacaagcgg tcagcgtcgc gactgtgccg caaaccgtct atgtggctcc tgcgccagct
     4861 ccggcttacc gcccgcatcc ttatgttcgc catcttgcca gcgtaggtga agggatggtt
     4921 atcgaacacc agattgacga ccatcaccat taattcgaga atgcctgatc gcacaaagca
     4981 atcaggcatt tttttaccct tcgtgagaat ttcccatctg tttatctttt aaaaagataa
     5041 ccatcagcga cagccacaaa attccgttga caaggttgaa gagattaaac agtccgttgc
     5101 ccccgttcag ccaggcatgt ttgctgattt caataccgac ggtaaagatc agcatttgca
     5161 gcattcccat cgccgcagaa accgtacctt tactcatatc gctggcaaac agggttaatc
     5221 gcaccagtcc cgcattcgcc agaccaatac cgaaagcata aatacttaac ccggcggtca
     5281 tccataaata cgcgtgcgat gagataaccg ttgccgcagc agcgaccaac agaccaatca
     5341 taatcggcca gccgcccata ataatcagcg aacgtacggt gcggcgcgag gtcagacgcg
     5401 ctaacagcaa gttacccgca attaacgccc cgaaaatagg cacttgcagc aaaccatatt
     5461 catagctgct caactgctcg ccggtaatga tgataatcgg cgactgggcg atccacgcga
     5521 gcaacggcag gctaacgaat cccagcgcca gcgcccccgc cacaaagcga ccgttcttca
     5581 gcaccagctt atagtcacga ccgagttctt tcagcgacag tttctcgcct atacgcgtgg
     5641 cggtttcagg catggctcgt tgcagaccga aaaaggagat cgctgccaat gcggcaaaca
     5701 agacaaacat cccttcccag ggcagcacat ggatccacgc cgcgcccacc agtggaccaa
     5761 gtagcggagc aatcagcgcc acgttcgcca tcagcgcggt gatcttgata caaaccgcct
     5821 cttcgaagga ttcctgaatt gcggcgtatc ccacagcgcc aatgaaacag aggcttatgc
     5881 cctgcaagaa gcgcaacagg gtgaattgtt caatgttttg cgccagtaat attgccagac
     5941 aggtgacaat aaaccacacc acacccgcca gcatcaccgg acggcgacca atacgatccg
     6001 acaacggccc caggagccat tgtaaaaaca tcccgcccgc cagatatgcg gtcatcgaag
     6061 taggaaccca atcaatgccc gcctgatatt gttccaccac ggccaacata ccgggttgaa
     6121 tcatatcgtt gccgatatag gttgaaaatt cgtacagcac cagacagaga gggaaaagta
     6181 acgcctgacg tccaagcctg gcaccggaag ctaatttatt ttgcatgcaa tttcttcgcc
     6241 aataataatc gcgcaaagtt taataaaagc gcagctaacg agaaagcgaa ttttgtagct
     6301 gaaaccgcgg ttaagcacat tcttacatta ttgcgagtat agctacgctt tctttaagtt
     6361 ttatttaacc tatgcccgtt acaatcaccc accgtaaaca ggccgcttga gggaaataag
     6421 acgatgccgc tttacccagt ttaacctgca ctttattctc aacgagttgc ctgtattggc
     6481 tcccttttaa tcactttgcg tcgggaagtt atgctggaaa atttgaatct ctctctattc
     6541 tctcttatta acgcgacgcc agactcggct ccgtggatga tctcgttggc gatttttatt
     6601 gctaaagatt tgattaccgt ggtgccgctg ctggccgcgg tactttggtt gtgggggctt
     6661 acagcacaac ggcaactggt gataaaaatc gctatcgcgc tggcggtcag cctgtttgtg
     6721 tcctggacga tgggacatct ttttccgcac gaccgaccct ttgtcgaaaa tatcggctat
     6781 aacttcctgc atcatgcggc ggatgactca ttcccgagcg atcacggtac ggtgattttc
     6841 acctttgcac tggcattttt atgctggcat cgcctgtggt ccggctcact tttaatggtg
     6901 ctggccgtcg tcattgcctg gtcgcgcgtt tatcttggcg tccactggcc gctggatatg
     6961 ctcggtggat tgctggcagg tatgattggc tgccttagtg cccagattat ctggcaagcg
     7021 acagggcata aactctatca acgtctgcaa tcgtggtatc gcttctgttt tgcattaccg
     7081 atccgcaaag gctgggtgcg tgactgatct ctgctaaaaa gtgtagtatt gagcggctcg
     7141 cttcaataac tattcagagg gattatggaa acacgtcgcg aagagcgtat cgggcagctg
     7201 ctgcaagaat taaaacgcag cgataagtta catcttaaag acgccgccgc gctgcttggg
     7261 gtttcggaga tgacgattcg tcgcgatctg aacaaccaca gtgcgcccgt cgttttgctc
     7321 ggcggctata ttgttctgga accgcgcagt gccagccatt acctgttaag cgatcaaaaa
     7381 tcccgcctgg tggaagaaaa acgccgggcg gcaaaactgg ctgcgacgct ggtagaaccc
     7441 gatcagaccc tcttttttga ctgtggcacc accacgccgt ggattattga agcgattgat
     7501 aatgaaatac ctttcaccgc cgtttgttat tcgctaaata cctttctggc gctgaaagaa
     7561 aaaccccatt gccgcgcatt tctgtgcggt ggtgaatttc acgccagcaa cgccattttc
     7621 aaacccatcg attttcagca aacgttgaat aatttttgcc cggatatcgc tttttattcc
     7681 gcggcgggcg tgcatgtcag taaaggcgct acctgtttta atcttgaaga gttgccggta
     7741 aaacactggg ccatgtcgat ggcgcaaaag catgtgctgg ttgtcgacca cagtaaattt
     7801 ggcaaggtgc gtccggcgcg catgggtgac ctgaaacgct ttgatattgt ggtgagtgat
     7861 tgttgtccgg aagatgagta tgtgaagtac gcgcagacgc accgtattaa gttgatgtat
     7921 taatgacgta taaccggatg acgtttcgca ccatccggtt atcagaagat taagagaacc
     7981 agctgccgaa ccactgatgg aatttcatca tcacgaaatc ccacatccga ccaaagaatc
     8041 cgccctcttc cacattttcc atcacgatca gcggacgctg ctcaatggat ttaccgttaa
     8101 gctggaaatc aatggtgccg acaacctgac cttttttcag cggtgcggta agctgcggtt
     8161 ccgttaacgt gtagctcgcc ttcaggtttt tcagctgccc acgcggtatg gtcactgagc
     8221 ccgcttcgcc tgccccgaga ttcacttcgc tcttatcacc aaaccagacg cgctgagtca
     8281 caaaggtggc atcaggttta attggcgtca cggtttcaaa gaagcggaaa ccccaggtca
     8341 ataatttctc agactcatta aaacggatac ggtcagtttt cgcccccagc actacggaga
     8401 ttaaacgcat atcgccctgg gtagccgaag caaccagatt atatcccgcg cctgccgtgg
     8461 ttcctgtctt catgccatca acattcagat tgctgctcca cagcagacgg ttacggttag
     8521 gctgacgaat tttgttgaag gtgaactctt tctctttatg aatggcgtac tcttccggca
     8581 catcgtggat caatgcttta cccagcaatg ccatatcgcg cgcggtactg aactgccccg
     8641 gcgcatccag accgtgcacc gtctggaagg tagtgttggt cagacccagt tttttggcat
     8701 aaccattcat caaaccaata aatgactcct ggctcccggc aacgtaatcg gccagcgcaa
     8761 tacaggcgtc attaccggac tggataatca cacctttgtt caagtctgcc accgaaacct
     8821 gatcgcccgg tttgaggaac attaccgatg aaccacgcag tgccggattt cccgtcgccc
     8881 aggcatcttt accgaccgtc accatatcgg tgagtttaat cttatcggcc ttaagcgcct
     8941 gcccaaccac atagctggtc atgattttag tcaggctcgc gggatccagt ttctcatccg
     9001 cattgccttc tgccagcact ttaccgctgg cgtaatccat taaaatccat gcacgcgcat
     9061 ccacgctcgg cgcttcaacg gtttgttcgg ccgcgaatgc cgttggggca aaaaggaata
     9121 aaaatgcaga acccgctgca agaccacgaa ggagagagga gtattgcgtc ataaatgcca
     9181 cccgagtatc cattcaaaaa aaatacgtta tatcgccgta tgataagttc ggtgattaat
     9241 aacgcacaca caaacttaaa gaaaccgtaa gttggtaaag tttttaaagt ttatgcaata
     9301 tcctgccgct tcgctgcttc tggtgtcatt tttttgccag ctgttgctta aaagttaact
     9361 ttacttcacc atcggagtcc tgttcagcct gtggctgcct gtcggaaaag gggtgagtat
     9421 gattacgctg tggggtcgga ataattcaac caacgtaaaa aaagtattgc tgacgctcga
     9481 agaactggaa ctaccttatg agcaaattct cgcgggccgt gagtttggga taaatcacga
     9541 tgctgatttt ctggcgatga accctaacgg gctggtgccg ttgttgcgtg acgacgaaag
     9601 tgatctcatt ctttgggaat caaacgccat tgtccgctat ctggcggcac agtacgggca
     9661 aaaacgcctg tggatcgact caccggcacg tcgtgcggaa gcagaaaaat ggatggactg
     9721 ggcaaaccag acactcagta atgctcatcg cgggatcctg atgggattag tcagaacacc
     9781 accggaagag cgcgatcagg ccgccattga tgccagttgc aaagagtgcg acaccctgtt
     9841 tgccctgctc gatgcggaac tggcaaaagt aaaatggttc tctggcgacg agtttggtgt
     9901 gggcgatatc gctatcgcac cgttcatcta caatttgttt aatgttggcc tgacctggac
     9961 accgcgtcca aatctgcaac gctggtatca gcaactcact gaacgccccg cagtgcgcaa
    10021 agtggtgatg attcccgtta gctaattgcg tgggctaact ttaagtaatt ccccactgga
    10081 ctcgtcggtg agaacgtata aataaccgtc gggtccagtg cgaacatcac gaattcgctg
    10141 ccctctgtcc gttaaaatac ggccatcttc tgtcactttg tcgccgttga cgctcatcac
    10201 aatgacatct ttatctttca gcgcgccaat aaataatttt tgctgccact gggggaattt
    10261 atcgctgtta tagaaggcca tgccgctcac ggcgggcgaa tctttccagt aaaaaacagg
    10321 ttgctcggtc ccggcgacga tctccccttt cgcttccggt atcttaaagc ctgaatagtt
    10381 gattccccag gttgccagcg gccagccgta gtttttgcct ttttgcggga tattaatttc
    10441 atcaccaccg cgcgggccat gttcattcag ccacagtgca ttactccacg gattcatcgc
    10501 cattccttgc ggattacgaa tgccataaga ccagatctcg gcgcgcgcac cggattcctt
    10561 tataaaagga ttatcatccg ggatttcgcc ctggtcggtc agacgcacca gtttgccctg
    10621 taatttatcc agatcctgcg ccgtcgggcg ctgattgttt tcgcccagag caataaaaag
    10681 ataaccttta ccgtcgaata ccagccgccc gccaaaatgg ttgccggtag acagttttgg
    10741 catctggcga aagacggtgc gaaagtcggt cacttttgag agatcatcac ttaagcggcc
    10801 ataacccaca gcagttccgg ctttgccatc atcgccaact tcggaataac ttaaccagat
    10861 gcggcgagac tgagcaaaat caggcgctaa aaccacgtcc agcaggccgc cctgcccgtg
    10921 cgcccaaacg tccggaactc cggaaagcgg cgcagataat ccttttcctg cttgccagtg
    10981 acgcaactcg ccgccgcgca gagtgattaa cataccgtga ttatcgggta aaaaggccag
    11041 tgcccaggga tggtcgagtt tgtcttgcag tacttcgaca tttaccgttg caggagccgc
    11101 ccagagagcg gaagaaagac aaataagggg cacaaggaaa aaggattgtc gatgcataac
    11161 gcctccggat tgactctggc ttaaagcgta gtcagtggag gagataacaa attcattttt
    11221 acaaaaactt aaacatgaag ggggagacgc tttctccccc ttagttttca ggccttctca
    11281 agcatggcgt gcttctgcag gctctggata ctcagcgtta agctcatcag acaattttca
    11341 agcttatcgg cgttgacggt aataacagtc gggcaatcat ggtgcccact catcaaacat
    11401 actgcggctg tcgctaatgc ttcttcagca tgatgaagag cactccactc ttcctgatcc
    11461 agatgaagat tcaaccgcag cgatttatcg tgcagttcgc gattcagttt aaaaaagtta
    11521 tctcgtagat gattgctttc gctgacggac atgtatcctt ttgccttcct cagttgcacg
    11581 tatgcggcga ggtcaacgcg tgcgtcgatc aaccggttca gcagatcgat tcgctgtctg
    11641 tcaacgaaca tacttcgatc ctcctctttc cagccttgtt acaattaaat agtatggcta
    11701 ttttttgagc aaaatgctgt ccaggatcaa ttcttcactc cgaattaaca aaatttacat
    11761 ttccttcgcc tccgccagcg aatgagattg cgatacaaat tttatccaga actctgtaga
    11821 atccctgccc tgacctttcc tgtaactgaa tattttttaa gctatgagca aagtaactcc
    11881 ccagccgaaa atcggctttg tttcccttgg ctgtccgaaa aaccttgttg attcagagcg
    11941 tattctcacc gaactacgca ctgaaggtta tgacgtggta ccgagctatg acgatgcgga
    12001 catggtgatc gtcaacacct gcggctttat tgacagcgcg gtacaagaat cactggaagc
    12061 cattggtgaa gcgttgaatg aaaacggcaa ggtaattgtg accggttgtc tgggggcaaa
    12121 agaagatcag atccgcgaag tccacccgaa agtgctggaa atcaccgggc ctcatagcta
    12181 tgagcaggtt ctggagcacg ttcatcacta cgtgccaaaa ccgaaacaca acccattcct
    12241 gagcctggtg ccagaacaag gtgtcaaact gacgccgcgt cattatgcct atctgaaaat
    12301 ttctgaaggc tgtaatcacc gctgcacctt ctgcattatt ccgtctatgc gcggcgacct
    12361 ggtgagccgt ccgattggcg aagtgttaag tgaagcgaaa cgtctggtag atgcgggcgt
    12421 taaagagatt ctggtgatct cgcaggatac ttccgcctat ggcgttgatg ttaaacatcg
    12481 tactggcttc cacaacggcg agccggtaaa aaccagcatg gtcagcctgt gcgaacagtt
    12541 atcgaaactg gggatctgga cacgtctgca ctacgtttat ccttatccgc atgtggacga
    12601 cgtcatccca ctgatggcgg aaggcaaaat cctgccgtat ctggacattc cgttgcagca
    12661 cgccagcccg cgcattctca aactgatgaa gcgtccgggt tctgtagatc gccaactggc
    12721 gcgcatcaaa cagtggcgcg aaatctgccc ggaactgacc ctacgctcaa cctttattgt
    12781 cggcttccct ggcgagacgg aagaagattt ccagatgcta ctcgacttcc tgaaagaagc
    12841 acgtctggac cgcgttggct gctttaaata cagcccggtt gaaggtgcag acgccaatgc
    12901 cctgcctgac caggttccgg aagaagtgaa agaagaacgc tggaaccgtt tcatgcagct
    12961 gcagcagcag atttccgccg agcgcctgca agagaaagtg ggccgtgaaa ttctggtgat
    13021 tatcgacgaa gtggacgaag aaggcgcgat tggtcgcagc atggcagatg caccggaaat
    13081 cgacggcgcg gtttatctca atggtgaaac caacgttaag ccgggtgata tcctgcgtgt
    13141 gaaagtcgag cacgccgatg agtacgattt gtggggtagc cgggtttaat taattcaggc
    13201 cgggcaaaat catttgtccg gcctgcgaat aatgattact gccagctaat ctgtgccctg
    13261 ccgtttttct tactcttata cagccgctca tccgccagca ttttcaagcg ttcgacggta
    13321 gtacattcat ttgaatgcgc tacaccgata ctggtgctta aattaatgct tcccacctgg
    13381 aaatgaacga tttcttttag aatcgatagc aactcttcca gcgttccacc cgtataaatc
    13441 accgcaaatt cgtcaccgcc gaagcggtag actttacctt tatctaccgg gatctgtgat
    13501 ttaaggattt tcaccacttt catcaacact tcatcaccca gcaaatgacc gaaggtgtcg
    13561 ttaatgtttt tgaatttatc gacgtcaaac aggcacaaat aaccgccttt ttccggcagc
    13621 gcttttaaat cttcgtcaaa ggattttctg cccattgctt tggtcaggtc gtccaggaac
    13681 gccgagttat acatactgta gagttttttg acatcggcag ccgaaaagaa aagcccgcca
    13741 tccagtacgc gttcaaaatg gttattaata atggcttctt tctccacaat accacgatta
    13801 atcactctgc gtaccagata gaggcaactg gtacccgata tcactgccac caatgcggca
    13861 aagaaaataa tcaatgtaca aacccgcaaa taaacatctt ttttatacgc aaatacgtac
    13921 aatccggtcg gcaaataggc gaagtgctcc atcgcttccg aaacggtgaa gatggcgttt
    13981 ttagcattat attcatcacc ggtcaccggt ttaccgttct taaacagaga gaacatcagg
    14041 ttctcgctat ccatcgccag agattttgac agtaaagaaa aacgctccag accaagcaaa
    14101 aatatcagac tacgattgtg agttaccgga tagaggtaat agacgaaggt tttaccgtca
    14161 aaagagataa accctgtatt tttgacatgt cgatacttca caacctgcat aatatctgca
    14221 caataatttt tctcgaactc ctttacctga atacattcgt cactggaaat cagataagga
    14281 ccgacatcgt acaaataaga gggtgaaacc gagtcggcgt aagctgtgcg taagccaacc
    14341 aacagctgtt cctccgtggg atttagcgtg ttgataagat caattgccac gctacgccga
    14401 tattcagcaa tatttgccac cgcaaaagca gtatcaataa cggactgttt caggctatac
    14461 acccgttcct ttaccatttg agtgtagatc ccgcaggcaa ctgctgaaat catgataaaa
    14521 atcagcagac tgactgtaag tacgaactta ttgattctgg acatacgtaa actactcttt
    14581 tactaatttt ccacttttat cccaggcgga gaatggcatg ggtttctggt acagataccc
    14641 ctgagcctga acacatccta atttgattaa ttcgcgcgca acgtcttcgc tctctacgcc
    14701 ttccgcaatc accgggatcc ggttatagcg tgccaggtcg aaaataatgc gcacaatttt
    14761 tcccgcagta ccgaaaatat cgccggtaaa ttgcgagtcc acctttatgc tgtctggatt
    14821 aatttcataa aaacgtttca ggtttgacaa accggtgcca aagtcatcaa tcgatatccg
    14881 taccccttgt ttacgcaatt gttcaatatt gagataaaaa tgttcattaa ttcgttcgat
    14941 tacggtttca gtgatctcaa cacagaggct gttcgccttc aggccatttt ctttcataat
    15001 ggtggtaaaa cgctcatgaa agtgtggttc gtttaactgc cagggcgaaa cattaatatg
    15061 cagtaagaaa tcatccttca gcgacgcacg ctgactaaaa tgtctgaatt cacccattgc
    15121 cgttttaata atctgataac ccagctcatt gataaacccg atatcttcag caatggtaat
    15181 gaacttcagc ggtgaaatga tcccccgctc cgcagacacc caacggcaca acgcctccgc
    15241 gcccagaatg gcgcgggtgt taatatcaac gatcggttga tagacgagga atagttcgcc
    15301 aatttccatt gcatggcgaa tattacgcgc aatccagata ttctcttcta tttcgttgag
    15361 tttcgcgtca tcacagaaaa taaactcgga gaacatatgg tcacggatac tggaaatcgc
    15421 cagtttcagt ttttctgcgt aactccaggc gttttccccc tgcaaaggtt cacaaatggc
    15481 agacttatgg atgcgcagat tgcggtagcc aaagctgctg tcaaaatcgt ttaccatcgc
    15541 tattttacgg gtaaatgtct gtacattttc ccgacacaga agtaaataga gatcggcatt
    15601 gaatgtcacg atatcgcagc attgtgcata tttctcggca attcgctgac acaatttcct
    15661 gacgatttta ttgcgttcat caacaccaaa caccgcttcc acggcttcca gaccacacag
    15721 tttgatgatc cccacagcca gatactgatt attgttggac tcaataatcg ccatattttt
    15781 aataaaccca ctttcctgat cgtaagagat cagtgagagc aatttatttg accgttcaat
    15841 ttcggcatct ttcatttccg cgatcgaatt tttgatgctg ataatctctt tcgcatcgcc
    15901 ttcgcgaatt ttcagagcct cgatctgatc cgttgagtca ggcaaaaacg ccagcgtatt
    15961 aaccagctct gtcatttcag aaaattgccg acggaacaca cgagtggtca gtaaataaac
    16021 caccagcaat acgataataa tgaacgcaaa cggcagataa aaaagtttat ctgatgcgtt
    16081 caataaatta ccgcccggat aata
//
LOCUS       DAZKFU020000098        15947 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZKFU020000098 DAZKFU020000000
VERSION     DAZKFU020000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.099"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..448
                     /locus_tag="ACHOQQ_RS12550"
                     /old_locus_tag="pgaptmp_002510"
     CDS             <1..448
                     /locus_tag="ACHOQQ_RS12550"
                     /old_locus_tag="pgaptmp_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGU4470747.1"
                     /translation="ESTEKPLMDNIHDTLSGLRRLDIDKRWDFLHFGLTGTSAFDPAK
                     NDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLESLDRNELRKQFSIKRLNEME
                     IYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHALTVIVG"
     gene            complement(515..1972)
                     /gene="nuoN"
                     /locus_tag="ACHOQQ_RS12555"
                     /old_locus_tag="pgaptmp_002511"
     CDS             complement(515..1972)
                     /gene="nuoN"
                     /locus_tag="ACHOQQ_RS12555"
                     /old_locus_tag="pgaptmp_002511"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGU4470748.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(1979..3508)
                     /gene="nuoM"
                     /locus_tag="ACHOQQ_RS12560"
                     /old_locus_tag="pgaptmp_002512"
     CDS             complement(1979..3508)
                     /gene="nuoM"
                     /locus_tag="ACHOQQ_RS12560"
                     /old_locus_tag="pgaptmp_002512"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGU4470749.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3672..5513)
                     /gene="nuoL"
                     /locus_tag="ACHOQQ_RS12565"
                     /old_locus_tag="pgaptmp_002513"
     CDS             complement(3672..5513)
                     /gene="nuoL"
                     /locus_tag="ACHOQQ_RS12565"
                     /old_locus_tag="pgaptmp_002513"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGU4470750.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5510..5812)
                     /gene="nuoK"
                     /locus_tag="ACHOQQ_RS12570"
                     /old_locus_tag="pgaptmp_002514"
     CDS             complement(5510..5812)
                     /gene="nuoK"
                     /locus_tag="ACHOQQ_RS12570"
                     /old_locus_tag="pgaptmp_002514"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGU4470751.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5809..6363)
                     /gene="nuoJ"
                     /locus_tag="ACHOQQ_RS12575"
                     /old_locus_tag="pgaptmp_002515"
     CDS             complement(5809..6363)
                     /gene="nuoJ"
                     /locus_tag="ACHOQQ_RS12575"
                     /old_locus_tag="pgaptmp_002515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGU4470752.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6375..6917)
                     /gene="nuoI"
                     /locus_tag="ACHOQQ_RS12580"
                     /old_locus_tag="pgaptmp_002516"
     CDS             complement(6375..6917)
                     /gene="nuoI"
                     /locus_tag="ACHOQQ_RS12580"
                     /old_locus_tag="pgaptmp_002516"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGU4470753.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6932..7909)
                     /gene="nuoH"
                     /locus_tag="ACHOQQ_RS12585"
                     /old_locus_tag="pgaptmp_002517"
     CDS             complement(6932..7909)
                     /gene="nuoH"
                     /locus_tag="ACHOQQ_RS12585"
                     /old_locus_tag="pgaptmp_002517"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGU4470754.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7906..10632)
                     /gene="nuoG"
                     /locus_tag="ACHOQQ_RS12590"
                     /old_locus_tag="pgaptmp_002518"
     CDS             complement(7906..10632)
                     /gene="nuoG"
                     /locus_tag="ACHOQQ_RS12590"
                     /old_locus_tag="pgaptmp_002518"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGU4470755.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10685..12022)
                     /gene="nuoF"
                     /locus_tag="ACHOQQ_RS12595"
                     /old_locus_tag="pgaptmp_002519"
     CDS             complement(10685..12022)
                     /gene="nuoF"
                     /locus_tag="ACHOQQ_RS12595"
                     /old_locus_tag="pgaptmp_002519"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGU4470756.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12019..12519)
                     /gene="nuoE"
                     /locus_tag="ACHOQQ_RS12600"
                     /old_locus_tag="pgaptmp_002520"
     CDS             complement(12019..12519)
                     /gene="nuoE"
                     /locus_tag="ACHOQQ_RS12600"
                     /old_locus_tag="pgaptmp_002520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGU4470757.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12522..14324)
                     /gene="nuoC"
                     /locus_tag="ACHOQQ_RS12605"
                     /old_locus_tag="pgaptmp_002521"
     CDS             complement(12522..14324)
                     /gene="nuoC"
                     /locus_tag="ACHOQQ_RS12605"
                     /old_locus_tag="pgaptmp_002521"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGU4470758.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14418..15080)
                     /gene="nuoB"
                     /locus_tag="ACHOQQ_RS12610"
                     /old_locus_tag="pgaptmp_002522"
     CDS             complement(14418..15080)
                     /gene="nuoB"
                     /locus_tag="ACHOQQ_RS12610"
                     /old_locus_tag="pgaptmp_002522"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGU4470759.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15096..15539)
                     /gene="nuoA"
                     /locus_tag="ACHOQQ_RS12615"
                     /old_locus_tag="pgaptmp_002523"
     CDS             complement(15096..15539)
                     /gene="nuoA"
                     /locus_tag="ACHOQQ_RS12615"
                     /old_locus_tag="pgaptmp_002523"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGU4470760.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT         3914 a         4546 c         4205 g         3282 t
ORIGIN      
        1 tgagtctact gaaaaacctt tgatggataa tatacatgac acgctttcag gattacgccg
       61 actggatatt gataaacgct gggatttttt acactttggg ctgacaggca cctctgcgtt
      121 cgatccagcg aagaatgatc cgctgagtcg tgccgtgctt ggtgaacaca gcctcgaaga
      181 tggtatagat ggtttcctgg gattgacatg gaatcaggag ttagccgcga caatcgaccg
      241 actggaatca ctggatcgaa atgaactgcg taaacagttt tccattaagc gactcaatga
      301 aatggagatt tatccgggag tgaccttttc agaagagtta gaggggcaac tctttgcttc
      361 gatcatgctt gatatggaaa agctgattag cgcgtatcga cgtatgctgc gacaagggaa
      421 tcatgcgctg acggttattg ttgggtgatg gtgtcagggc accaaaaaaa accgccgaac
      481 atgtcggcgg ctttctgact tacaaactaa cagattacat cagtggcatt gctaaccgca
      541 caatgctaat cagcggttgt ggccatacac ccagcaccag taccaacagt gcggagatca
      601 gcaccacgat accgcccgcg ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt
      661 ccggtgcgtg caggtacagg ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg
      721 aaccgacaac cacggcaccc accagccacc acaagtgtgc ctggacaccg accgccagca
      781 cgtagaactt accgataaag cccagcgtca tcgggatacc ggccagagac agcatcatca
      841 ccgtcatcac cgccgcgagg atcggacgat gccagaacag accgcggtaa gagaacaggg
      901 aatcagcatc cgggccacga tacgggctgg acatcaggct gaccacgccg aacgcgccga
      961 ggctgctgaa cagataaccg gccaggtaaa cccctaccgc ttccatcgac atctcgccgg
     1021 tttgcagcgc aatcagcgcc accagcagat agccgaggtg agagatagat gagtaaccga
     1081 gcagacgttt gatgttggtc tggctcagcg ccatcaggtt accgaagatg atggaggcaa
     1141 aggcgataat cgccagcacc acccgaatcg cttcgctgtc acccaccggt gcgtagagga
     1201 acagacgcat caccacaccg aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa
     1261 ccggcgcagg cgcgccctgg tatacgtctg gcgtccacag gtggaacggc accagagaga
     1321 gtttgaagcc gaggccaaca atcatcaggc cgaaacctgc cagcaacagc ggctcgttga
     1381 gcataccgtc gccgaggttt ttacccagcg cgacaaacga caggtcgcca gactgcgcat
     1441 acaccagcgc cataccgaac agcaggaaag aagacgctgc ggcagaaagg atggtgtatt
     1501 tgatactggc ttccagcgaa cgtttctggc ggaaagcgta accgaccagg ccaaacagcg
     1561 gcaaagagat cagttcaata ccgaggaaca gagacgccag atggtttgca ttcgccagca
     1621 ggatcccgcc cagcgcggca attaacacca acaggtagaa ctcatccttg ttgtcgttat
     1681 agccttcaag ccacgggtag gcgaaagtac aggtggcgag gctcgccaac aataccagcc
     1741 cggtgtaaag catggcgaaa ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg
     1801 cctggccaac aaaccagagc gaaaccagcg ccgcgttaag cccaataacc gagagcgtag
     1861 cgttgaggaa atgattgcgt cgccacgcaa tggagagcat cacaaccacc accgtcaagc
     1921 cgacgatcag caacggtagc agtgcgatca ggttttgtgg agttattgtc atggcgattt
     1981 acggccttgt agtagtaacg gaattaacaa accactgctg gatattgcca atcgcggagt
     2041 gcgaggtatc cagaatcggc tgcggataga agcccagcag taccagcagc accaccagca
     2101 acaggatcat aaacagctca cgcagcgaca tccctggcag ttcctggctg gcaatctggc
     2161 ttttcgcttt accgaagtaa gcgcgatgta acatcgccag cgaataaaca gatgcaaaga
     2221 ccagcccaaa ggtagagata acggtaatca ccgggacaac ctggaagctg ccgaacagaa
     2281 tcataaattc gccgacgaag ttaccggtgc caggcatccc aagcgttgcc accgcaaaga
     2341 acagcgacag tgctggcagc catttcatct tgctccacag accgcccatc atgcgcatgt
     2401 cgcgggtatg gatacgttca taaagctgac cgcagagaat aaacagaccc gccgctgaca
     2461 agccgtgcgc aatcatctgg attaccgcgc cctggtaggc caactggctg ccggtgtaga
     2521 tagcaatcag tacgaagccc atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat
     2581 cggtctgggc gaaggccatc cacgcaccgt agaagatgcc gataacaccc agccacatag
     2641 cgattggcgc gaactctgcc gacgcatttg ggaacagcgg cagggagaaa cgcagcaaac
     2701 cgtaagcggc agttttcagc aagatccccg cgaggtcaac ggaaccggcg gtcggagcct
     2761 gggagtgcgc atccggcagc cagccatgca gcggaaccac cggcattttg actgcgaagg
     2821 cgatgaagaa gcccagcatc aacagatatt ccacaccact ggacattggc gtattcaaca
     2881 gctcttcata gttgaaggtc cagacgccgg tcgcattgta gtgaacaaaa accagcgcca
     2941 ggatggcgat caacatcacc agaccactcg cctgggtgta aatgaagaac ttggttgccg
     3001 ccgtgatacg cgttttaccg tcagaggctt tatgccccca cagtgcgatc aggaagtaca
     3061 tcggcaccag catcatttcc cagaagaaaa agaacaggaa catgtcgatg gcaaggaaca
     3121 cgccgataac gccgcccagg atccacatca ggttgaggtg gaagaagccc tgatattttt
     3181 cgatctcttt ccacgaacag agtaccgcca gcacaccgag cagaccggtc agcacgacca
     3241 ttagcagcga cagcccgtca atggcgagat ggatagagat accaaaacgc gggatccacg
     3301 gcatgtcgaa ttcagactgc cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc
     3361 cctgcaacca cagttgcagc gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc
     3421 agcgcggcac cttgacgcca aagcgttcgg tctgccagca caggaagccg ccgataaagg
     3481 gaattaatat tagccagggt agtaacatgg cgatctttat tccttgtaaa agtcccgtca
     3541 ggaccggatt ttcaacgaat tctcacgaca gaaaattctc ttcggactgg gggtagtgtc
     3601 ggatgcaccg cgaaggaggc ttatccgacc tacgttctta tatgcccagg ggcaatccca
     3661 caatccttaa ctcaacgcag taccatcaac agtgccagca ccacgaccgc accgatgctc
     3721 atggatgcca cataccagcg cagataaccg ttctcgctta acagcaggcc tttacctgca
     3781 aagcgggaaa gaaccgccgg aatgttcatc attgagttca gcggatcgcg tttcagcaac
     3841 caggcaatac ccaggaacgg cttgacgaac actttgtcgt acagccagtc aaagccccag
     3901 gcgttgtacc accaggtgcc cagcagacgg cccggcgcac tgttggcgat ggaggtcacc
     3961 agagtacgtt tacccagcca cagccaggct gccagcagaa tgccgaccac cgcgaccacg
     4021 ccagaggtaa tttccagggt caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc
     4081 acgccctgca gcggcggtac aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca
     4141 atcagcggca ggctgtgagt tacccctttc acggcgtgag cgtgaatttg ttcttttccg
     4201 tggaagacga tgaaaatcat acggaaggtg tagagcgagg tcataaacgc accgaccaga
     4261 cctgccacca tcagattgat atgaccattc gccatcgcac ccgcgaggat ctcatcctta
     4321 ctgaagaagc ccgcagtgac cagcggcagt gccgacagtg ctgcgccgcc caccaggaag
     4381 cagagataaa ccagcggaat agatttacgc agaccgccca tcttgaagat gttctgttcg
     4441 tgatggcagg ccagaatgac ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg
     4501 tgggtcatca agtgaaaaat cgccgcatcc catgcctgca cgccaagcgc gaggaacatg
     4561 tagccaatct ggctcatggt agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc
     4621 gcggcaaaac cggccagcag cagcgtaacc gccccgacaa tacccaccag atgcagaact
     4681 tccggcgtca tcaggaacag gccgtgggta cgggcgatca ggtagacacc cgcggttacc
     4741 atcgttgcgg cgtggatcag cgcggagaca ggcgtcgggc ccgccatcgc gtcggcaagc
     4801 catgtctgca acggcaactg cgcagattta ccgaccgcac cgcccagcag catcagcgtc
     4861 gcccacatca gcatattatt gccgtcagca aagtgcgctg gtgccagttc caccatttcg
     4921 cggaagttca gggtgcccag ttcgttgtaa agaatgaaca gcgcgaaagc gaggaacacg
     4981 tcacccacac gggtcacgac gaacgctttc attgccgctg cgccattctt cggatcggtg
     5041 taatagaaac cgatcagcag ataggagcac aggcccacgc cttcccagcc gaggtacatc
     5101 agcagcaggt tgtcggcaag caccagaact accatgctgg cgatgaacag gttggtgtag
     5161 gcgaagaagc gagagtagcc ctcttcaccg cgcatgtacc aggaggcgta catgtggatc
     5221 aggaaaccca caccagtgac caccgagagc atggtcagcg acaggccgtc cagcaccaga
     5281 ttgaaaccga tgttaaagtc gcctaccgac atccacgtcc acagcggctg gctgtaagcc
     5341 tgctcgccgt tagcgaagaa atcaacgccg ataaaagcgg ttaccagcgc cgccaggccc
     5401 acagagccaa cgccgacgat cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc
     5461 agcaggacga agccaatcaa tggcaaaata atggttaagg caagcatgtt catccgcgca
     5521 tctcacttac tgaatcgatg ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg
     5581 caaggccgat actcgcttct gccgccgcga ggctgatggc gagaatgtac atcacctgac
     5641 cgtcggtctg accccagtag cttccggcga ccacaaaggc cagcgcggag gcgttaatca
     5701 tgatttccag accaatcagc ataaacagca gattgcgacg gataaccaga ccggttaagc
     5761 caagaacgaa taagattgcc gcgaggatca gtccatgttg taaggggatc atgcgtgctc
     5821 ctccgttttt cttttcgcgc tgtcgtcttt acgattgctc agcacttcac ccgcacgctc
     5881 ttcacgaccg acgtggaagg ccacaaccag acccgcgagc agcagcatag aagccagttc
     5941 caccgccagt acgtaaggcc cgaacagcgt aatacccact gctttagcac tgattggcgt
     6001 accgtcgata ccttgatcgt taacaccgag aatggcgtaa acaatcacca ccaacatgat
     6061 ggctgacaaa attgccggac caatccacac ctgcggtttc agccactggc gttcttgttc
     6121 gatttctgaa ccgcccaggt tgagcatcat caccacgaac acgaacagca ccataatggc
     6181 acccgcgtag acgataattt ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac
     6241 cccggaaatc gccagcagcg aaataatcag gtacagcagt gcgtgtaccg gattggtatg
     6301 ggtgatcact cgcaaggtcg caagtatggc tatcaggcca cagatataaa aagcgaactc
     6361 cattgcccct ctccttacgg taacaggctc ttgacgtcga taggcttggc ttcgttctct
     6421 gcttcgccct tatctttgcc gtcgattgcc atacctgcca tccggtagaa gttatattcc
     6481 gggtatttgc ccggaccgga gatcagcaga tcctctttct cgtaaaccag atcctggcgc
     6541 ttgtattccc ccatttcgaa atccggggtt aactgaatcg ccgtggtcgg acaggcttct
     6601 tcgcacaaac cacagaaaat gcagcgtgag aagttgatgc ggaaaaattc cggataccag
     6661 cgaccgtctt tggtttctgc tttttgcagc gagatacagc cgaccgggca ggctaccgcg
     6721 cagaggttac aggctacgca acgctcttcg ccgtccgggt cgcgggtcag aacgatacga
     6781 ccacgataac ggggcggcag atagaccggc tcttccgggt acattcgcgt ttcgcgtttg
     6841 gcgaacgcgt gcaggccgat catccagata ctacgaacct gggtgccgaa acctactaac
     6901 aattctttta aggtcatggt cttattgccc cttattgcgc ctgccagaga atgacagccg
     6961 ccgttaccag caagttgatc agcgtcagcg gcaggcagat tttccagccg aaggacatta
     7021 cctggtcata acgcggacgc ggtaacgacg cacgaatcaa aatgaacatc atcataaaga
     7081 acgcggtttt cagcgcgaac cagatgaatg gcggtaacaa cgggccttgc cagccaccga
     7141 agaacagcgt caccatcaat gcagagatgg tcacaatccc gatgtactca cccacgaaga
     7201 acagaccgaa cttcatgccg gaatattcaa tgtggtaacc atccgccagt tcctgctcgg
     7261 cttccggctg gtcaaacggg tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa
     7321 taaaaccaaa gaattgcggg atgacgttcc acacatgcgc ctggctgttg acgatgtcgg
     7381 tcatgttgaa tgaaccggct tgcgccacca cgcccatcag ggaaagcccg aggaacactt
     7441 cgtagctcag ggtctgcgca gaagcacgca tcgcacccag cagcgagtat ttgttgttac
     7501 ttgaccagcc cgcaaacagc accgcgtaaa ccgccaaacc ggccatcatc aggaagaaca
     7561 aaatcccgat gttcaggtcg gcaaccaccc aacccggact gactggcaca atcgcaaagg
     7621 ccagcagcag tgaggtaaag gcaatcatcg gtgccagggt aaagatgacg cgatccgaga
     7681 atttcgggat ccagtcttct ttaaagaaca ttttgatcat atccgcgacc agctggagcg
     7741 aaccgcccca gccaacacgg ttaggtccgt aacggttctg gaacagaccc agcaggcgac
     7801 gttcgccaaa gctcatgaat gccccgcagg tgacaaccac cagcaggatc accaccgctt
     7861 tgaggatggt cagcaggatc tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc
     7921 cttgagatcc tcaagatgcg cgccagccag caccggagca atgccggaca tacccatcgg
     7981 caagcccacc tgccctgccg tcagcccttc agcgatttca accggcagcg tgaccgtgtt
     8041 gccatcgtaa ctaaaggaga cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc
     8101 tgggttgagt ttgatgtacg gctgcggcat acggctctgg aagaccggag cacgctgtga
     8161 caattcatca ctgccaaaca ggtggtaata cggcgcaata cgccatttcc cgtcctgcgg
     8221 ctggaagcgt gccggtacgc tggtgaagta atccagacca ttctcgctgg tttcaaacag
     8281 acgcacgccc ggatcgccaa agcgcagttt gccgcccact tcgtcctgga atttgttcca
     8341 cgcctgcggg gagttccagc ctggcgccca ggcaaacggc acttgcgaac ggtgcgcggt
     8401 cggctggttg ttaccttcca tcgagaaggt gaacatggtg tcaatatcct gcggctgacg
     8461 cggctcatga acgctaatat tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc
     8521 acgggccagt ttctgcccac gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc
     8581 tgccagttcc gggattttcg ccacaacagc gtcaatcaca tggtcgagct gcgtccagtc
     8641 cacttcacgg ctcaacaggg tactgtgcag cgagtgtaac cagcgccagc tttccagcat
     8701 gacagttttg ctgtcgtaat aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc
     8761 ttcattatta attaccgtac cgtcgctttc agcaaagctg gcagcggaaa gtaccagatg
     8821 ggcgttttcc atgatcgctg tgcgttgatg gtcaaccacc atcaccagcg gcgctttagc
     8881 cagcgcagca ttcacgcggg tagcagaagc gtgacgatgc agatcgtttt ccagcaccac
     8941 caccgcgtcg gcgcgtccgg tttccagttc ggttaacgct tcttcaagcg atccaccgcc
     9001 cataatgccc agccccatgc tgttgacgga acgggcaatc atggtgatac cgacgtcagc
     9061 gccgcgacct ttcagggctt tcgcgacgtt agccgcagcc tgaattactt cagcgctacc
     9121 ggcgttcgtc ccggagataa tcaacggttt cttcgcaccc gccagtgcct gcacgatgac
     9181 gtcgattttg ctttgcagtt caggttcaat accgtcaacc gctggcgcgg agttatccag
     9241 cgcatgggcg atggcaaaac ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca
     9301 cgccgcgata tcatccagac gggtgtcatc aacgttggta acaaacagcg gatgcttcgc
     9361 acgttgaccg atgttgagga ttgccgcaat ctgccagtca gccactttct gtgctgccgc
     9421 catttcgcgc gctttacctt tcaccgcctg acgcactgcc agcgcgacgc gcgcgccggt
     9481 ctgggtaacg tcttcgccca gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc
     9541 aggagtataa atgccgcctt cacgcagtac tttcagcgcc agttgcagac gttcctgctc
     9601 accgtgagcg ataccggtgt agaagttttc ttcgcccacc agctcacgca gcgcaaagtt
     9661 actttccacg ctggcgcgcg gagaaccaat gccgatcact ttcttcgact gacgcagaat
     9721 atctgctgcg ccctgcattg cctgttcggc gttgagggta atgaaatcat cgccacgacg
     9781 ctgtactggc tgacgcggac gatctttcag gttgacgtaa ccgtaaccga aacgaccacg
     9841 gtcgcagagg aagtagtggt ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc
     9901 gccataacgc tcgccggggc tgatgttaca gccgatggaa cattgctggc agatgctcgg
     9961 cgcaaactgc atatcccatt tacggttgta acgctcggag tgcgttttgt cggtaaatac
    10021 gccggtcggg caaatttcga ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc
    10081 cgggcgaccg aagtagacgt tgtcgtgcgc gccataaacg cccagatctg taccgtcagc
    10141 gtaatctttg tagtaacgca cgcagcggta gcaggcgatg cagcggttca tttcgtgaga
    10201 gatgaacggc cccaaatcct gattacggtg ggtacgtttg gtgaaacggt agcgacggaa
    10261 gctgtgtccg gtcatcacgg tcatatcctg aagatggcag ttaccgcctt cttcacatac
    10321 cggacagtcg tgcgggtggt tggtcattaa ccactcgacc acactttcac ggaactgttt
    10381 cgcttcttcg tcgtcaatgg aaataaaggt gccatcggaa gccggtgtca tacaggacat
    10441 caccaggcga ccacgcgtgt cttccgcgtt ttggtattgc ttcaccgcac actggcggca
    10501 agcaccgacg cttcccagcg ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga
    10561 cagacaagct tccagcaggt tgtccgctcc gttgacctcg tattctttgc cgtctacatg
    10621 aattgtagcc attagcatgc ttccagtttt ctcagtcaga gactgagcgt taatcgaaat
    10681 tcggttacca gcgctctttc agcaggttcg gctgaatccc attaatcaaa tgggtattgc
    10741 tgaacggctg tttgatcccc gcctcaaatt cttcgcggaa atatttgatg gcgctctgta
    10801 acggctccac tgcaccaggt gcgtgggcac agaaagtttt acccgggcct aagaatcgac
    10861 acagttgctc aagtgtttcg atatcgcccg gctgaccttc gccacgctcc agcgcacgca
    10921 gaattttcac gctccacggc agaccgtcgc ggcacggcgt acaccagccg caggactcac
    10981 gggcgaaaaa ctcttccagg ttacgcacca gcgacaccat gttgatctca tggtcaaccg
    11041 ccatcgccag cgccgtaccc agacggcttc ccgctttacc gatactttcg aattccatcg
    11101 gcagatcaag gtgcgcttcg gtcaggaagt cagtccccgc gccgcctggc tgccaggctt
    11161 taaatttcag accatcacgc ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg
    11221 tgccgaacgg cagttcccac agtcccggat ttttcacccg accggagaag cccatcagct
    11281 tggtgccagc atctttactt ttcgagatgt tctgatacca ctccacgccg ttagcgagga
    11341 tcgccggaac gttacacagg gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc
    11401 cggaggttgc cgggaagggg ggtttcgagc gtgggttagc acgacgccct tccagggagt
    11461 tgattaacgc tgtttcttcc ccgcagatgt agcgccctgc cccggtatgg acgaacagtt
    11521 cgaagtcgaa acctgttccc ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg
    11581 caatggcgcg gcgcagattt actgccgctt cgatatattc gccgcgcagg aagatgtagc
    11641 cacggtaagc tttcagcgca aacgcggaga tgagcatacc ttccaccagc aggtgcggca
    11701 gttgctccat caacaggcgg tctttatagg tgcccggctc catttcatcg gcgttacaca
    11761 gcaggtaacg gatgttcatg gattcgtctt tcggcatcag gctccacttc aggccagtcg
    11821 aaaagcccgc gccgccgcgc cctttcagac cagcgtcttt tacctgatta acgatttcgt
    11881 ccggagacag cccggtcagc gccttacgcg ccccttcgta accgtttttg ctgcggtatt
    11941 cgtccagcca cactggctgt ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg
    12001 gagtacggat aatgtttttc atttataccg ctccagcagt tcagggatcg cttccggggt
    12061 cagatgcgcg tgagtgtcct catcgatcat catgtttggc cctttatcac agttccccag
    12121 gcagcaagtt ggcagcagcg taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt
    12181 cagctttttc tcgagcgccg cctgaatacc ctgatacccg ttgatatgac agaccacgct
    12241 gtcgcaataa cggatcacat ggcgaccaac cggctgacgg aagatctgac tgtagaacgt
    12301 tgccacacct tcgacgtcgc ttgccggaat acccagcaca tcggcgatcg cgtggatcgc
    12361 accatccggc acccagccac gctgcttctg aacgattttc agcgcttcaa tggacgccgc
    12421 acgcgggtct tcgtagtggt gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag
    12481 ctcaaaagcc tcggtttgtg gttgttgatt ctcgtgcata attagcggtc cacatctgac
    12541 ataacaaaat cgatactgcc cagataaaca atcaggtcag acaccaggct gccgcggatc
    12601 gccgccggaa tttgctgcaa atgcgcataa ctcggcgtgc ggatacgagt gcgataactc
    12661 atggtgctac cgtcgctggt caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc
    12721 tggaaagatt cattggcagg catcaccggc ccccacgaca cttgcaggaa gtgggtgatc
    12781 agggtttcga tatgttgcag cgtgcgctct ttcggcggtg gcgtggtcag cgggtgatcc
    12841 gctttgaacg ggccttccgg catgttgttg aggcactgct caagaatgcg cagactctgg
    12901 cgcagctctt ccactttcag cattacgcgg gtgtagcagt cagaaacacc accaccaacc
    12961 gggatttcaa agtcgaagtt ttcatagcca gaataaggac gcgccttacg cacgtcgaag
    13021 tcgatcccgg tagcacgcag gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg
    13081 ccataggcgg caacgccctg ggaacgacct ttcagaatgg tattttgcag cgccgctttc
    13141 tcgtaagacg ccagacgttt cggcatccag tcgaggaact cacgcagcag gcgatcccag
    13201 ccacgcggca ggtcatgcgc tacaccgcca atacggaacc acgccgggtg catacggaaa
    13261 cccgtgattg cttccaccag atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg
    13321 ggcgtcattg cgccgacgtc ctgaataaag gtcgagatat acagcaggtg actgttgatg
    13381 cggaacagtt cggagagcat tacgcgaatg acgttaacgc gatccggcac ggtgatcccg
    13441 gccagtttct ctaccgccag cacgtaaggc atttcgttaa cgcagccgcc gaggtattcg
    13501 atacggtcgg tatacggaat gtagctgtgc caggactggc gttcgcccat tttctccgca
    13561 ccacggtggt ggtagccgat gtctggtacg cagtcgacaa tctcttcgcc gtcgagctgc
    13621 aaaacgatac ggaaagcacc gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg
    13681 aagtcctcgt tttcggtgcc gcgcttcatc ccccactctt ccggtttgaa ggtcagggct
    13741 tccatctcca gatcctgttt ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc
    13801 gccgggtaat ctttacgcag cgggtgacct ttccaggttt gcggcatcat gatgcggcgc
    13861 aggttcgggt gaccgtcgaa tgtaatgcca aacagatccc aggtttcacg ctcataccag
    13921 ttagcgttcg ggaacagttt ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc
    13981 accttcagca tgatgtcgcg gttacgatcg atagaaatca gatggtagaa aacggaaaaa
    14041 tccgcggcag gtaacccttc gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca
    14101 aacagcatga cgtaaggttt cggcagtttc tttaagaaat cgccaacttc cagtaattgt
    14161 tcacgcttga tccacacaac gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc
    14221 ggcccaaaac ggttgcgcag ttcgccaatt accggatcat caagatgatc gcgggtctgc
    14281 caggcgggtt cttgcgcggt taagtcggtc atattgttca ccattgcaaa tggtccgtgg
    14341 tgactgtcgg cgcagtaagc ttcgcgaaat agatttagga ataagcgaag aaaaatccct
    14401 ttgccgacag gcgcaaatta aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt
    14461 tcaccgcgct tgcgttcgcg ctctgattgc atattggcgc gataaacgcc ctgatcgcca
    14521 accacccagg agagcggacg acgttctttg ccgatagatt cctgcaacag catcagtgcc
    14581 tgcatgtacg cttcaggacg cggcgggcag cccgggatat acacatcaac cgggatgaat
    14641 ttatcgacgc cctgcacaac ggaataaata tcgtacatac caccagagtt ggcacaggca
    14701 cccattgaga taacccattt tggttccagc atctggtcat acagacgctg aataaccggt
    14761 gccattttgg taaagcaggt tcctgcaacc accatcaggt cagcctgacg cggcgaagca
    14821 cgcaatactt ctgcgccaaa acgcgccacg tcatgcaccg cggtaaacga agtcaccatc
    14881 tcaacgtaac agcaggaaag accgaagtta tacggccaaa ttgagttttt acgaccccag
    14941 ttaaccatgt cattgagctt gcccataaac acgtttttgt taacttcttg ctccagaggg
    15001 tcggttacga tctcctgctt ttgcaggggg taacggtcgt tctcaccgtt gggatctatg
    15061 cgggtgagcg tataatccat cttaatgcct cgcggttagc gttgacgatt agcgatactg
    15121 ttcgtttccg ggttcatacg ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca
    15181 atacgcacca gataaaccag acctgccagt aacacaaaaa taaaaattgc agcttccaca
    15241 aagcctaccc agccgctttc gcggatagag gttgaccatg cgaacagata cagcgcttca
    15301 acgtcgaaga taacgaagaa catggccacc agataaaact tggcggacag gcgtaagcgg
    15361 gcggagccga ccgagtcgat accggattca aacggcacgt ttttcgacct cgcgcgtgcg
    15421 cgaccgccta aaaaccaacc gcctaccagc atcaggcaac acaggccaat ggcaacgata
    15481 agaaagatag cgaatgccca gtgatgagcg atgacttcag tggatgttga catactcatt
    15541 gcttactcat caaaagtagc gccagattca ctgctcttca cggcagatgg acgccacatc
    15601 gattcatggg gaggaataaa aaaaacctta caatcactgt agaaattctt ttatacagct
    15661 aattgatgtg gtcttttact cctttctata accttttgtc aactttaaca aaagcttctt
    15721 cacattagtt tacataatat caacaccatt agcatttaat gccctttcac cccagatcct
    15781 tgacgactcc aggataatta gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa
    15841 atacgggtct attttgacag gaatttgtgt cgattcctcc ccccaaaaga gagtattttc
    15901 ttgatctgtg acacgctttt gtcattccat aacaaaaacg cagcaac
//
LOCUS       DAZKFU020000099        15931 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZKFU020000099 DAZKFU020000000
VERSION     DAZKFU020000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.100"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            7..621
                     /locus_tag="ACHOQQ_RS12620"
                     /old_locus_tag="pgaptmp_002524"
     CDS             7..621
                     /locus_tag="ACHOQQ_RS12620"
                     /old_locus_tag="pgaptmp_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGU4470761.1"
                     /translation="MGKDLEEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATES
                     VSSGISSPAVETHIPEAPARKKKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRA
                     KGGGFLPLKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRY
                     NLDGQPEGEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
     gene            complement(842..2326)
                     /locus_tag="ACHOQQ_RS12625"
                     /old_locus_tag="pgaptmp_002525"
     CDS             complement(842..2326)
                     /locus_tag="ACHOQQ_RS12625"
                     /old_locus_tag="pgaptmp_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4470762.1"
                     /translation="MRKIIAHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSS
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSTPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(2635..3039)
                     /locus_tag="ACHOQQ_RS12630"
                     /old_locus_tag="pgaptmp_002526"
     CDS             complement(2635..3039)
                     /locus_tag="ACHOQQ_RS12630"
                     /old_locus_tag="pgaptmp_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HGU4470763.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHSWDDYVDNFFR"
     gene            complement(3207..3494)
                     /locus_tag="ACHOQQ_RS12635"
                     /old_locus_tag="pgaptmp_002527"
     CDS             complement(3207..3494)
                     /locus_tag="ACHOQQ_RS12635"
                     /old_locus_tag="pgaptmp_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4470764.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHILSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            complement(3491..3742)
                     /locus_tag="ACHOQQ_RS12640"
                     /old_locus_tag="pgaptmp_002528"
     CDS             complement(3491..3742)
                     /locus_tag="ACHOQQ_RS12640"
                     /old_locus_tag="pgaptmp_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4470765.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            4227..5093
                     /locus_tag="ACHOQQ_RS12645"
                     /old_locus_tag="pgaptmp_002529"
     CDS             4227..5093
                     /locus_tag="ACHOQQ_RS12645"
                     /old_locus_tag="pgaptmp_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU4470766.1"
                     /translation="MDISTLLLDFRITSHCNLGCDLCFRTPGIQDSSLASARNVIERM
                     YKMGFRRIGFTGGEPTSRSDFIDLIEYAKQLGFMTYLSTVGHRFIMDHARLNPVLDWV
                     GIPIDGIDSLVNSEIRSDKMSNQHRVIKNILTWLPQNNNRINIKLTTVVSQSNINHLS
                     DIVCWVDSLPYKIQAWRFYQFCPLGVGKEKRDKLEINTDIFKEKMVMLKKQHPNMPIS
                     WATFEERDKANVVMEPNFDIIIPDGENYTLLGNMLTDSQDHIISAIFGNNEIMQKCKN
                     NRFWLDKMEFTV"
     gene            5090..5797
                     /locus_tag="ACHOQQ_RS12650"
                     /old_locus_tag="pgaptmp_002530"
     CDS             5090..5797
                     /locus_tag="ACHOQQ_RS12650"
                     /old_locus_tag="pgaptmp_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014727861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HGU4470767.1"
                     /translation="MRYNYLYMDPWSADKKEKNKTRNPLYICLSGNSGVGKSTLLRRM
                     SKELFKVDPDTVAIDEKSVHHTLLPYLFDDTSNYGYLIQLNFMIQRALLIKSWLDKGF
                     NLIMERSHIEDYIFINFMLKSEYINKAQYDAYMVLWSRINELLTEPDIIAFINYPIAH
                     SLMHLNDDEIHGVRPEEFPDEEMKIKWITGWHAEYQHFTDNLPLHLRERIMVCAHPDD
                     IDDFSDKVIQKAMMLRT"
     gene            5799..6224
                     /gene="nudI"
                     /locus_tag="ACHOQQ_RS12655"
                     /old_locus_tag="pgaptmp_002531"
     CDS             5799..6224
                     /gene="nudI"
                     /locus_tag="ACHOQQ_RS12655"
                     /old_locus_tag="pgaptmp_002531"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGU4470768.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            complement(6757..7398)
                     /locus_tag="ACHOQQ_RS12660"
                     /old_locus_tag="pgaptmp_002532"
     CDS             complement(6757..7398)
                     /locus_tag="ACHOQQ_RS12660"
                     /old_locus_tag="pgaptmp_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4470769.1"
                     /translation="MFIRAYLRASTEDQFADRAKEMLEQFVQERGHKIASYYRENISG
                     TKLDRPELGRLLMDSHRNDILLVEQIDRLTRLSNSDWITLKKQIEQHELRIVSLDVPT
                     SWQALSDKDPSQSDPITRAVITAINNMLIDLMAAMSHKDWLSRRQRQKQGIERAHTLG
                     KYRGKQADQERHQKVLYYRQVKKLSIRETADATGYSTSQVCRIQALYREPTTD"
     gene            complement(7689..8171)
                     /locus_tag="ACHOQQ_RS12665"
                     /old_locus_tag="pgaptmp_002533"
     CDS             complement(7689..8171)
                     /locus_tag="ACHOQQ_RS12665"
                     /old_locus_tag="pgaptmp_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470770.1"
                     /translation="MRNKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     AARLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG
                     "
     gene            complement(8288..9136)
                     /locus_tag="ACHOQQ_RS12670"
                     /old_locus_tag="pgaptmp_002534"
     CDS             complement(8288..9136)
                     /locus_tag="ACHOQQ_RS12670"
                     /old_locus_tag="pgaptmp_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4470771.1"
                     /translation="MNNDELVTRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMRGKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEEMTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLEI"
     gene            complement(9182..9446)
                     /gene="yacB"
                     /locus_tag="ACHOQQ_RS12675"
                     /old_locus_tag="pgaptmp_002535"
                     /pseudo
     CDS             complement(9182..9446)
                     /gene="yacB"
                     /locus_tag="ACHOQQ_RS12675"
                     /old_locus_tag="pgaptmp_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            complement(9446..9715)
                     /gene="yacA"
                     /locus_tag="ACHOQQ_RS12680"
                     /old_locus_tag="pgaptmp_002536"
     CDS             complement(9446..9715)
                     /gene="yacA"
                     /locus_tag="ACHOQQ_RS12680"
                     /old_locus_tag="pgaptmp_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HGU4470772.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            10348..10710
                     /locus_tag="ACHOQQ_RS12685"
                     /old_locus_tag="pgaptmp_002537"
                     /pseudo
     CDS             10348..10710
                     /locus_tag="ACHOQQ_RS12685"
                     /old_locus_tag="pgaptmp_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            10714..11069
                     /locus_tag="ACHOQQ_RS12690"
                     /old_locus_tag="pgaptmp_002538"
                     /pseudo
     CDS             10714..11069
                     /locus_tag="ACHOQQ_RS12690"
                     /old_locus_tag="pgaptmp_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            11350..12489
                     /locus_tag="ACHOQQ_RS12695"
                     /old_locus_tag="pgaptmp_002539"
     CDS             11350..12489
                     /locus_tag="ACHOQQ_RS12695"
                     /old_locus_tag="pgaptmp_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HGU4470773.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            12609..13877
                     /locus_tag="ACHOQQ_RS12700"
                     /old_locus_tag="pgaptmp_002540"
     CDS             12609..13877
                     /locus_tag="ACHOQQ_RS12700"
                     /old_locus_tag="pgaptmp_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU4470774.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEMISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            13885..15030
                     /locus_tag="ACHOQQ_RS12705"
                     /old_locus_tag="pgaptmp_002541"
     CDS             13885..15030
                     /locus_tag="ACHOQQ_RS12705"
                     /old_locus_tag="pgaptmp_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HGU4470775.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            15498..15644
                     /locus_tag="ACHOQQ_RS12710"
                     /old_locus_tag="pgaptmp_002542"
     CDS             15498..15644
                     /locus_tag="ACHOQQ_RS12710"
                     /old_locus_tag="pgaptmp_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470776.1"
                     /translation="MAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYR
                     GMSM"
     gene            complement(15715..>15931)
                     /locus_tag="ACHOQQ_RS12715"
                     /old_locus_tag="pgaptmp_002543"
     CDS             complement(15715..>15931)
                     /locus_tag="ACHOQQ_RS12715"
                     /old_locus_tag="pgaptmp_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HGU4470777.1"
                     /translation="MPIRGLLIGNEEKAITICKKMHDRGFATTVAMYPTVEEGKAILR
                     CAISAIHTRENIDSLHKNFIECINEQG"
BASE COUNT         4178 a         3835 c         3673 g         4245 t
ORIGIN      
        1 gtatttatgg gaaaagatct ggaagaaaag aaaaatcccg tcattgtggt gaaaaaacgt
       61 cgctcttttt caccgccttc cttagccgaa aaaaaagatg ttatcgcgcc tgcacttact
      121 gaacaggcga cagaatcagt ctcctccgga atcagttcac ctgccgtcga aactcatatc
      181 cccgaagcgc cagccaggaa gaagaagaag aaaaagaaaa gacaacgctt tccaagacca
      241 tcccactgga ccagtgagtt cactcacgat tgtctggaga aggtgaaagc gctgtttcct
      301 caacttcgtg ccaaaggagg cggttttctg cctctgaaaa caggcataac ccgtgacatt
      361 actacatatc tggcagcaca tccggaaaca gcgctgaccc tggacgaatg gaattgtgca
      421 gtatcctgca ttaccagtcg ccggatctac ctgcagagaa cctctgttgc aggaactccc
      481 cgttataacc tggatggtca accagagggg gaagtatcgg aatgtgatgc tcaaaattcc
      541 aggcgctggc tggcggcgaa agagcagagg tggctgagta tgaaggcccg gcaggaaaat
      601 atttctgaga aatcaaaata accgcaggat attacaggaa acagactgta gagaagagag
      661 cgttgtatat tgtgcgtatt atttaagcac tcccgagagt aatggcttga ttttgcgcga
      721 tcgcgacatt ttttacgtgt atccagtttg acgtttctgg tgccgtgatc gcactgaacg
      781 cattttgctc ccaatgcaaa atatgtccgg tatcaggagc aaatggcggg caatttttaa
      841 actattttat atctatttca gcatttcctg gacaggatat atttcccatt ttcatagggg
      901 tgataagatg acgaacaacg gccttatcta cacataaatt gactccggta agagctaatg
      961 tatgcccatc accatttatt gtcagcaacg ggctggaaaa tttctctgcc atcttacgag
     1021 cattaatcca tggtgttgtt ggatcgtatt tgtgtgctac aaacagtaaa ccagagggca
     1081 gaactgtatt tttcagccgg gttttattca ggttgctatg tattggccat aattcacaaa
     1141 aatcaggtga atcggaacgt ccattgtcaa agttaatagc cgggaaggca ttcgcaagag
     1201 cgtcttttcg ggattttcgc tcttctgggg ttaattgctc ccccccctga tcaacacaaa
     1261 ggattacccc cagagcatca cttgactctt ctgaggctat aggagtactg agcgcagttt
     1321 caatttcatt actgacaatc ccctgagaga actgacgtat ggcagttgca agggttggcc
     1381 atgatgaacg ccataacaaa aggtctgttg ttaataatat gagttcatct gaagatatat
     1441 tttctccctt gctgtctaat aaaggcttgt gatgcaattt tgataacagc tcatggaact
     1501 gagttattgc cttatctctg actgaagaaa gtgggcaact ttttgtacgt gcacaccagg
     1561 atgcaaagcg atcgaacgtt tcctgataac tctgtgcttg tttgagttgc catgtgaagt
     1621 tgtcccccag gtcatcaata tcgacgaccc catcaagaac gatagatctg atgctggatg
     1681 ggaaacgttc tgcatataag gctgcaattt gtgttccata cgaatatgcc acagctgtca
     1741 gttgctcgtc acccagggct tgcctgatac gatcaatatc atataccgcc tcgttagaac
     1801 caatatggcg aataacttca gttccggtat tatggataca gctgttaatt ttatttaata
     1861 cccgttgctt ttcagttatg ttttcctgaa tccctgtatc tgattgctgg cagtttattg
     1921 caggaatgga ctggccgact ccccgtggat caaagccaat aatatcccat gattcgcgaa
     1981 gatttgttac cggccagtca aagttaatat aaggattaat gccaggtaat cctggtccac
     2041 cacttattat aatgatactc cctttatgcc tgctttttgc tggcaatttt gtcagcgcca
     2101 gttttacctg ctgtcttttt acagaagaac tatctccgcc tgtgtctgta tattttaatg
     2161 gaacagacaa ataaccacat agtaagtcag gagacggttt ttcctcacca aaccagttac
     2221 tgaatggagt ggtcatacag gattgccact ctatcgactg ggcagatact gttgcgggca
     2281 gaagtaaggt taaaactatc ttgaaatgag caattatttt tcgcattgtg tctcttaata
     2341 tcggtataaa gataaagttt gaatatctta aatccggtgt tacggcagga agttacacgt
     2401 tacggatata attcaaatga ttacaaagtc agggtatatt gtttaactcc ccggaactat
     2461 gtaagcgtca ccgtcttgtg acgtcttatg atggttattc tgtctggtaa caatcggtta
     2521 ttttcctggt atcccactga cagcaaaaga acaagactgc tgaactataa acggccatga
     2581 tgaatcatca cgattcggcc ccagacttgt ccgtcaggta atttttcggt tcaatcaccg
     2641 gaagaaatta tcgacgtaat catcccatga atgcccctga acagaccatt catgattaac
     2701 cggtggcggg aatccacttc gacagtagcc gttcttcccc tcaaaatcga ataccccatg
     2761 tggatatccg tttatcagca gaacacactt caggcaatcc tcagaccagc caatcttcac
     2821 atcagagggg atgtgagcat ctgaaatgtc ttcaacgtta tatatatgca cagcatccct
     2881 gacagggtta ccttcaacgg actcatcaag tgcatagaaa tatccggtat tcccgtcgtc
     2941 ttcaaataca gcggccaaca ttccatcagg agctaaactt tttatcgcca tagcctcacc
     3001 aactatgagt tgagcctgtg ccgtcacaag tattgtcatc aaacatgtcc ctccgtttca
     3061 atttgtctgg taatataaat aaccccagac tcatcccggc aggtaaaatc attctctcaa
     3121 caaaactttt ctttttccgc ctcaggcgag gccacagaga gaataacaat tctgagagtt
     3181 atctgcggga aaggctattt atgttactat ctcattctct cactggcgag ataataaacg
     3241 ttactacgtt ctcgtttacc taccgcaaca accgcaataa ttaacatatc atcaatcacc
     3301 tgataaacca gacgaaatcc cgatgcacga agtttaattt tgtagcagtc ctttagccca
     3361 cgtagttttg cagacagtat atgaggattt ttgctacatt tttttagttt tttcacaaac
     3421 tgttgttgaa tggtcttatc cagtttcagc cattctttaa gcgcatcatc cctgaatttt
     3481 atcgtataag tcatgaataa ctgtccaggt caacatcatg cagaggttga ctgctacgtt
     3541 cagtaaccag tttcaccagc tcctggtcat ccagagcatc aagcatcctt tcatacagtt
     3601 cagcaggaac acagtagaat gccggctgat tgcgattcag aatggcgacc ggataaccat
     3661 caccagcacc gactgtcgcc atcggatttt ttttcagttc actgacactt gcactgatgt
     3721 cagacagaat gatgttaggc ataatgctcc ttttaagact gtttaataag tcttaaagat
     3781 acatctgaaa ggacccgtta acaagtctgt attactcata catttgcaaa taaatttaat
     3841 gctgattttt tactctgatg ccgcaccagc gcatggaagg tggcacacag tggttacata
     3901 taacacagga gtaaaatcgg cgtaagccgg tagaagaact aaatattctc cagttatctc
     3961 ccgtcttgtg aggcatacgg cctgtcagtg atgaaaatgc ttctgtgcac aattttcact
     4021 ggtgtgactg atagcgtccc tccgatcatg ggcttccagt aatgcgatta ccggaactca
     4081 tcattcatga gttccttgcg caacccactg ctctgattac tggcagatat tatatcctag
     4141 gtaagccgaa ggttgcgctt tgtcatggtc tgcaataacc attatgtttt aatggttatt
     4201 gatgtatcat agtcagagga cttgttatgg atattagcac ccttctttta gatttcagaa
     4261 taacttcaca ttgtaaccta ggatgtgatc tgtgttttcg tacacctgga attcaggact
     4321 cttctcttgc tagtgctcgt aatgtaattg agcggatgta taaaatggga ttccgtcgta
     4381 tcggttttac cggaggggaa cctacatccc ggagtgattt cattgatctg attgaatacg
     4441 ctaagcaact gggttttatg acatatctct ccacggtcgg gcatcgattt attatggatc
     4501 atgctcggtt aaatccggtg ttagactggg taggaatacc aattgatgga attgacagtc
     4561 ttgtcaattc agaaattcgc agtgataaaa tgagtaacca acacagggtt atcaaaaata
     4621 tattaacatg gttaccccaa aataataatc gaattaatat aaaactcacg accgtcgtat
     4681 ctcagtctaa cattaatcat ctcagtgata ttgtttgctg ggttgattct ctgccctata
     4741 agatccaagc ttggcgattt taccagttct gtcctctagg agtcggaaaa gaaaaaagag
     4801 ataaactgga aatcaatacg gatatattta aagagaaaat ggtaatgcta aaaaaacaac
     4861 accctaacat gcccattagt tgggcaactt tcgaagaacg ggacaaagcc aatgttgtta
     4921 tggaacccaa ttttgacatt attatccctg atggagaaaa ctatactctg ttgggaaata
     4981 tgctgacaga ttcacaagat catataatca gtgcaatttt cggaaataat gaaatcatgc
     5041 aaaaatgtaa gaataaccgc ttttggttgg acaaaatgga gtttacagta tgagatataa
     5101 ttacttgtat atggatcctt ggtcagcaga taaaaaagag aagaataaaa ctcgaaatcc
     5161 tctgtatata tgtctgtcgg gtaattccgg tgtaggtaaa agtactcttc tgagacgcat
     5221 gtcaaaggag ttatttaagg tagatccaga cactgtcgcg atagatgaaa aaagtgtcca
     5281 ccacactctc cttccttacc tttttgacga taccagtaac tacggttatc taatccagct
     5341 taattttatg atacaacgag cattactgat aaaatcttgg ctggataaag ggtttaacct
     5401 tattatggag agatcccata ttgaggatta tatttttatc aacttcatgc tgaagtccga
     5461 atatataaat aaagcacaat atgatgcata catggtgctc tggagtagaa taaatgaatt
     5521 acttacagag ccagacatta tagcattcat taattatccc atcgcacatt cactcatgca
     5581 tctcaatgat gatgaaattc atggtgtacg gcctgaagaa tttccagatg aggaaatgaa
     5641 gataaaatgg atcacaggat ggcatgcgga atatcaacat tttactgata atcttccgtt
     5701 acaccttcgt gagcggatta tggtatgtgc tcacccggat gacattgatg atttttctga
     5761 taaggttatc caaaaagcca tgatgttgag gacataaaat gcgccagaga gtaatagtgt
     5821 gccctgtgat acaaaatgaa ggtgagtttc tactttgtaa aatggcctct gatcgcggag
     5881 tattcccggg ccaatgggca ctgcccggtg gaggaatgga acccggagag actatggaaa
     5941 cagcattgag gcgtgaaata aaggaagaac tgggaaaaga actgcatatc actgacgtaa
     6001 aaccctgggc ctttcgtgat gatatccgta caaaaacgta cccggatggt acccacgagg
     6061 acatttacat gatttaccta atattcgact gtatcagtac aaaccgtaca gttacattta
     6121 atgaggagtt tcaggatata gtctgggcat ctcctgacac cctaaaatat atggatttaa
     6181 atgaagcaac ccggatcaca ttttctcaga aaggtatcct ttaaactcca atgctaatcg
     6241 tgcaactgat aacggcttcc acaacaggga gccgttttct tatgccacta ctcaaggatg
     6301 gggtttgagg gccaaccgta cgaaaacgta cgatttcgga cagcgaaaca gctgttgtcc
     6361 cgccatccag acgattcctg aaagtcattt gtctaaaaaa tagtcttaac gtggtttatt
     6421 ttccgaattc caagcggccc cctggatgag cgtgatgatt actggcggac ctttgacgtt
     6481 ccagcaaatt cccctgttcc acataggttt ccatatatga ccggttattt tcacgttcgg
     6541 tgtacgtgtt acagctgcaa ataatacagg tgcattatca atatcatcag ggataaaatt
     6601 gtaagatcgt ggaaaatgtt tattcatagc ccggatgatg gctgtctgtg catcctttgt
     6661 tcgggcgcgg tgtttccatt cacgccagcc atccacctca gaaataataa ggggtcagtt
     6721 tggatatcga aaattattgt acgttaaggc tactttttaa tcagtcgttg gttctcgata
     6781 aagtgcctga atacggcaaa cctgcgaagt gctgtagccc gtagcatctg ctgtttctct
     6841 aatgctgagt ttttttactt gtctgtagta caacactttc tgatgccgtt cctgatcggc
     6901 ttgttttccc cggtatttac ccaacgtatg agctcgttca attccctgtt tttggcgttg
     6961 gcgacgactc agccagtcct tatgcgacat cgcggccatc aggtctataa gcatattatt
     7021 tattgcagtt atcactgcgc gggtaatcgg atctgattgt gaaggatctt tatctgaaag
     7081 ggcttgccat gatgttggaa catccagact gacaattcgc aattcatgtt gttcaatttg
     7141 ctttttcagc gttatccagt cgctgttgct aagacgggtc aggcggtcta tctgctcaac
     7201 cagtaaaata tcattacggt gactgtccat cagtaatcgc cccagttccg gacggtcaag
     7261 ttttgtaccg cttatgttct cccgataata actggcaatt ttatgccccc gttcctggac
     7321 gaactgttcc agcatctctt ttgcccgatc tgcaaactga tcttctgttg atgcccttaa
     7381 ataagctctg atgaacattt tttcaccgca ttatcgcatt taggttgttg catatattct
     7441 atcgcatata ggttatgtca ttaattgtat gaggtcacat tttatatctc gcatcagggt
     7501 gtaccctatc gcgacaggca agcctgttca aaaaacaggc ttgccgtaca ataattctct
     7561 atatccaaac tgacccctat gagatcagtt tggatattcg aaattattgt acgtttaacg
     7621 caccatcatt aatgctaatg tatgcataca ttagcattaa tggtgaagga aaagaataac
     7681 aatggcagtc aacccacttc ttttttggat ttcttaattt tcgtctgata aagccgcttt
     7741 acccgttcaa tttctgccag cgttgcggac cagtgtagtt cggcggcatg acgatatctg
     7801 gcagccattt tttccaccat ctccggattg ctgtccgcgc tgcggtgctg acgccccttc
     7861 tccccaaaaa cttcaacaac cagccactgg cttccggttc tgccgaacaa tgccttgcac
     7921 aaacgtctca cagtcagcgg ggttaaatct ggatcgggat cataggcaat ggtagcaaaa
     7981 ataaagcgca gcatttctga tgtcttgtaa cgctttacgc tggcagccag gcgtgcggca
     8041 cgttgcggtg cttcccagcg gtcgttactg taattagcca cttcagccag ataatccttt
     8101 tcaagttttt cagccgtgta gcacctgccg cctaaaaaaa gccgcatggc ctccctggtt
     8161 ttatttctca catatcatcc ctcattaatg ctaatgtacg catacattag cattaacata
     8221 ctgacagtaa caagcggcgg atttacgtcc ggataacgta aatgattgcg gtatacaggc
     8281 caggcggtca gatttccagc cgtgcctgtt cctgcagcag ttccagatga tatgcagcaa
     8341 tagcgtttac cacccctgcc gtcatccgtg cgtcggcgat cgctgagtgt gcctgccctt
     8401 cccaggtcag tccggcctgg ctggcagcac aggccagtga aatcgtgcca tagcggttgg
     8461 tggccccata atatcctgca gccagctcca tcgcacaata taccgtcatt tcttccagcc
     8521 agtcagccgg atcgctatgt gcggcagcag tctgtttcag aatgcggatg tcgaaccggg
     8581 cattaaagat aattactggt cggtccccga ttgcatgccg cagttgacgc gccacatctg
     8641 tccatgatgg tgcgccgcag agagcctgct cactgatacc atgcacggcg gcagcctgcg
     8701 ccccgatggc gaccgtgggc ttcagcctgg tttcaaatac cacctgaccg gccgcatcgg
     8761 tcaggccgat ttccagggct tccgcggtat tatccagtcc tgtcgtttca gtatccagaa
     8821 agagtggtgc cagactcagc cagtcatatg cctgccgtgc catccggcca ctggtgctgt
     8881 ttagtcggga taaaacggaa agccgctggc cagccagctg ttgtttactc gtcagagggc
     8941 gtttcccacg catcggaaca cagtctgcaa ttcggtatac agcgaacctg ccaccataag
     9001 tatttttata ccacttaacc ggctcagcac cgggggctgg cttcattctg aattcatcgc
     9061 gcagacgtcc tttcgagaaa cacctgtctt cggcaatcgc ctgcgcacgt ctggtgacca
     9121 gctcatcatt attcatggtt cttgcatctc cgtcagacgt ttttatatcc ggacagcccc
     9181 tctatggcca cttctgtgca gtatgcaata ctcgcaggat atacactttt ccccactggc
     9241 tgtcgacctc ataaaccaga acaaaatgcg ggtgaataac cagttcccgg gtaccttcca
     9301 cacgaccgtt gcggccttta tacggctgcc cggccaggct gctggctgaa taaccaatcc
     9361 gttccagcca tcaggttctg ctccgcaatg tactcacgaa tgcgcttcct gtcctggctg
     9421 gccagcatgg tccagaatat ctccatcatt taccggcaac cttcctgctc atttcatccc
     9481 gccatgctgc agcagaggat tcgaccatgt catggggaag cacatcgccg tgctcgagtt
     9541 gctgacgtcc tgccgcaacc tggtcgcgga accaggtatt atgctgccgt tcaacggtct
     9601 gccgcataaa atcccggatt aactgagagc cattacggtc catgcttttt gcagcggcca
     9661 taaaagcatc cttcagttca gcgtcgattc ttaaactcat attaacctgt gccattcatc
     9721 acctcgtcat taccacgtat atacacagta tataattatt cagatatcgt caccatgata
     9781 tgccgcgcca gcaacatgga aggcggcact ccatgctgta tatggcagag gagtaaaacc
     9841 gccgaatccc ggcgtaagcc ggtacagtcg tccgaatgtc agtagttcta ctcccccctg
     9901 cgaggccagt gctgacgcgg gacatggaac gggtgctgga gcgaagtggg gaagctccgg
     9961 tatactggcc aaacctacac gcctccttcc gtctccggga agatgctggc ttcgtaccct
    10021 gcgtgcgtct gatctgtagc tggccgtggc acggctacgg acagattctg ctccgcaaca
    10081 gtatcggcgt gcctgacctc ctgaaagtcc cgtaacgcat tttctcacct cccatgccct
    10141 gacgctattg agcgcgttac agaacaattt aagtcattta acgagcctcc aacggctcga
    10201 tcgaaataaa tcaaatcgta atcgtcaacg atatatgaga ggagaccccg ctcgccgcaa
    10261 atcgatgacc gaacgtagtg agcgaatcga cgaggaagcg gaagagaacc tgtacgaaaa
    10321 tggagcaatt acgccccacc gtcaagaaat gagctggctc tccacggcgc gccatcatag
    10381 acagatgtgg ctctgcaact gcgccatgcg atcggagacc gaccagtaat tatctttaat
    10441 gcccggttcg acacccgcat tctgaaacag actgctgccg cacatagcga tccggctgac
    10501 tggctggaag aactgacagt atattgtgcg atggaactgg cagcaggata ttatggggcc
    10561 accaaccgct atggcacgat ttctctggcc tgtgctgcca gcaggccgga ctgacatggg
    10621 aagggctggc acactcagct gatcgccgac acacggatga cgtcaggggt ggtaaacgct
    10681 attactgcat atcatcagga attgctgcag cttatgtact gcgataaccc tgaactcaac
    10741 tatgcaaacc tgaaatccct gcaaaaacag ctggatgaaa tgtatacctg catccgtaat
    10801 aatcagatga tgctcccaaa ctacggaaaa atgcgccggt atggtgaacc ggtttcaaca
    10861 acgtttgtag agtcgacaat caacgaagtg atcgccagac gatgacccaa aagcaacaga
    10921 tgcagtgggg tagggaaggt acgcattatt tgttacagac cagaaccgct gtcctgaata
    10981 attaactgca ggacaaattc gccagttggt acccgggttg cacgatagac gctcagagtg
    11041 gaggaaagtt atccgccatg gcagcataac tacttcccca gttttttcag ctctccctta
    11101 ccaatatcct acgacgatca catcgaaaaa aaacacggaa caggcgaaca gaaatataat
    11161 taattatttg agtttaaagg aaaaaagagg atacaagctt tttgttgtat ttttatttgt
    11221 ataaaccgac aaaaaagagg ccatacttcc cgctttcttt gtctgtttac acggaacatt
    11281 tcaccactgg atacgctaat agatcggata tgagcacata ttgcgcccgc ctttcatgca
    11341 gagcataaaa tgaacaagat tcacgctaat tattacctgc aagattctac cgcacgccag
    11401 atagttcaac gcacaatggg gattattccg tactcggtaa atgtgatgaa cgagcatggg
    11461 atgatcatcg cttccgggga agcctcacgt ttacatcaac gccatgaagg tgcggtactc
    11521 gcattgaccg aaaaccgggt cgtcgaaatt gattccgcga ccgccaataa gcttaaaggc
    11581 gttaagcccg gaatcaattt accgattaat tttcgcgacc agctgattgg tgtactgggc
    11641 attaccggcg agcctggtga agtgaaggca tatgcacagt tggtgaagat ggcttcggaa
    11701 ttaatcattg aacaaatggc tctcctcgag caaaaacagt gggataagcg ttaccgcgaa
    11761 gagctgatta atcagctgat tttacgcgat ccgccaccgg actcactgaa tgcggtggtc
    11821 tcatggttgg gaattaatct ccagcttccg cgggtggtgg tgattatgga actgcaacaa
    11881 cctgatcgcg acgcactgca taatctgatg gagtattttg aatatcgcgc ccgcgaccat
    11941 ttggtcacgt tttccgactt taatgatctg ataatcttaa aacctatctc gctaaaaaac
    12001 ggcgtctggg ataaatcgtt ggaattaagc gatctgcaga gacttaaaca ttacgcccag
    12061 tcctgtggtt tttcccgact gattgtcggc ggctatttct ctggagaaaa cggcctgcat
    12121 cgctcttttc agaccgcgaa atccacccag tccatggcga aaagactgaa gttgaagcat
    12181 cagttcattt ttcatgacga ctatgcgctg gcatcattat tagatgggct ttctggtacc
    12241 tggcaagcgg aagaattatc caaaatctgg cgcaatctgg ttgccaagga caccaaaggt
    12301 atgctgcaaa aaacgctgca gaattatttt gatcataatt gtgatctttc gcaaacagcg
    12361 actggcttgc atattcatgt caatacatta cgctatcgac tgcagagagt ggaagatatt
    12421 acaggaatac aaatcaataa cttaaagcag ttattctggc tgtatatcgg catggagtta
    12481 caacgctgaa ctgtatttcc ctccaaattt ttcgccgtct gacgtcgtat ttttagccga
    12541 tacacacaag tctaacgcgg ctgctttctc catactgccc cctgttattt tcatggagaa
    12601 agcagaagat ggtttcaata acagcaactg gcgccatcat cgcactcgtg gtggcgatcg
    12661 ttttaatttt acgcaaagtc tcgccggctt acggcatgat tgtcggtgcg ctggtaggcg
    12721 gtctggtcgg cggcgccgat ctggtgcaaa ccgtgacgct aatggtcact ggcgcgcaag
    12781 gcattaccaa tgccgtgctg cgcattctcg cagcaggtgt actggccggc gtgttgatag
    12841 aatccggcgc ggcaaacacc atcgctgaaa ctgtggtgaa aaaagtgggg gaaagccggg
    12901 cgcttttagc cttagctatt gcgacgatga ttctgaccgc cgtcggagta tttatcgacg
    12961 tggcggttat cactgttgcg ccgattgccc tgtctattgc ccgtcgtgcc ggtttgtcga
    13021 aactcgccat tctgctggcg atgattggcg gtggtaaagc gggtaatgtt atgtcgccta
    13081 accccaatgc tatcgccgtt tcggacacct ttcatgttcc cctcacctcc gtgatgatga
    13141 ccgggataat ccccggtctg ttcggcctga tcgtggctta tttcctggca aaatgtctca
    13201 gccagaaagg cctgatggtg atgcccgacg aaatgatttc acacgacaac cacggcaagc
    13261 agccgggatt tcttgccgct atcagcgcgc cgctcgtcgc cattgtgctg ctggcgctac
    13321 gacctgttgc gggtatcgtc atcgatccgc tgattgccct gcccgtcggt gggttagccg
    13381 gtgcgatcct tatgggcagg tttaagcaaa gtaacgcctt tatgatgtcc gggcttacgc
    13441 gcatggcgcc ggtagccatc atgctgctcg gtaccggtac gctcgccggg attatcgcca
    13501 actcggaact gaaagatgtg ctcattcatg gactgacctc ttccggtctg ccttcttatc
    13561 ttctggcccc tgtttccggc gcgatcatgt ccatggcaac agcctccacc acggcgggaa
    13621 ccgctgttgc cgccggggta ttcagccaga cgctgctggg gcttggcgta tcagcccttg
    13681 ccggttccgc catgattcat gctggcgcaa ccgtacttga tcacctgcct cacggcagct
    13741 tcttccacgc cacgggcggc agtgtcggca tgctgatcca tgaacggtta aaactgctgc
    13801 cttatgaaac ccttgtcggt tttgtggtcg ccctgatttc cacactgatg tttggcgtct
    13861 tcggacttgc cgggtaaaga cacgatgaaa atagtcattg caccagattc atttaaagaa
    13921 agtttatcgg ccatgcaggt ggccaccgct attgagcagg gatttcgtga aatttatccc
    13981 gaggccgagt atgtcaaatt gccgatggct gacggcggtg agggtaccgt cgaatcaatg
    14041 gttgaagcaa ccgaaggcca ttatctgttc cagaatgtca ccggaccgct ggggacgcca
    14101 gtacaggcgc gctggggtat gctgggagat gggaaaacgg cgatcgttga aatggcctcc
    14161 gcatccggac tacatcatgt tcctccagaa tgccgtaacc ctatgcttac caccagttat
    14221 ggcacaggcg aactgatcct tgccgcactg gaccacggcg ttgagcgaat tattctcggg
    14281 attggcggta gcgccaccaa tgacggcggc gcaggtatgc tacaggcact tggtgtggcg
    14341 atgcttgata caaacgggaa atcgcttcct ttcggcggcg gcgcgctaag caacctggcg
    14401 ctcatcgatg cctcaggctg cgacgctcga ctgaaaaacg tttctgtcac cgtcgcctgc
    14461 gacgttaaca atccactctg tggcgcaaca ggagcgtcag ccatcttcgg cccgcaaaaa
    14521 ggagcaacac cagaaatggt tgcctcctta gaagctgcgc tgaccaactt cggtatacga
    14581 cttcaggacg caaccggacg aaacgtgatg acgacgcctg gcgcaggtgc cgctggcggc
    14641 atgggcgcgg cgctgttagg gattctgaat gcggagttaa agccgggagt ggagattgtt
    14701 attgacgcgc tgcatctcga tagcctggtc gcggatgcca gcctggtaat caccggtgaa
    14761 ggtcgtcttg atagccagag catcagtggt aaaacaccga ttggtgtcgc acgctgcgct
    14821 aagcggtatc agaaaccggt gatcgcactg gcgggcggct taacgaccga ccaccagata
    14881 gtgcatgagc acgggctgga cgcggtgttc tccgtgctga cgcaaatcgc cactctgccg
    14941 gaagcgctac aggatgcgga atacaacctc cgcgtttcgg cacgcaatat tgccagcgtc
    15001 tggaaattat cacagcagca gatgcgctaa tcttcagtca ggccggcaag cagtagctgg
    15061 cctggatatt ctttctcacc aggcccatgc taaaacagcc tgcttcagtc atcccacgca
    15121 aggcactagc atggcaaaaa aagaggggag tagtggatcc caaaagacgg ttaagcggtt
    15181 aaattgaatg gtcaataagg gggctgtttg ggaaacgaat ctatggtcac tcccgttttt
    15241 gcaacaccga ttttgacgat aagtgggctt gcttgaatct atccggcgtc tgaatgggat
    15301 tttattcccg cgcctcgatg agttccgcgc ctgatgaacc tcaagaaaat atacggcttc
    15361 aatgagcctt tccgtttcac aggttcctca acaggccggt gggccgttca tatcatcaat
    15421 accgtgatgc tgatactccg tacccaccgg aaccgcacct gatgcggttc cggcttaagg
    15481 gctgtcagga ctcgcttttg gctgccatca acaaaccgga agagatgccg gattatctct
    15541 tccgtcaact cggttttaat cagacctggc atgagtggaa gcgggacgaa cagcacaggc
    15601 aacaacaacg ccggcccgga cactaccggg gcatgagtat gtaatgtcag ggacagctat
    15661 acgccataaa aaaatgaaat agatggcctc tctataaaga ggccatgata tttactaacc
    15721 ctgttcgtta atacattcta taaagttttt atgtaggcta tctatatttt ctcgggtatg
    15781 aatagctgat attgcacaac gtagaattgc ctttccctct tccactgtag gatacatagc
    15841 aacggttgta gcaaaaccac gatcatgcat ttttttgcat atcgttatag ctttttcttc
    15901 attgccaatt aacaatccac gtattggcat t
//
LOCUS       DAZKFU020000100        15801 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZKFU020000100 DAZKFU020000000
VERSION     DAZKFU020000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.101"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1037)
                     /gene="yahK"
                     /locus_tag="ACHOQQ_RS12720"
                     /old_locus_tag="pgaptmp_002544"
     CDS             complement(<1..1037)
                     /gene="yahK"
                     /locus_tag="ACHOQQ_RS12720"
                     /old_locus_tag="pgaptmp_002544"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HGU4470778.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRT"
     gene            complement(1124..1201)
                     /locus_tag="ACHOQQ_RS12725"
                     /old_locus_tag="pgaptmp_002545"
                     /pseudo
     CDS             complement(1124..1201)
                     /locus_tag="ACHOQQ_RS12725"
                     /old_locus_tag="pgaptmp_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1414..2796)
                     /gene="yahJ"
                     /locus_tag="ACHOQQ_RS12730"
                     /old_locus_tag="pgaptmp_002546"
     CDS             complement(1414..2796)
                     /gene="yahJ"
                     /locus_tag="ACHOQQ_RS12730"
                     /old_locus_tag="pgaptmp_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGU4470779.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(2806..3756)
                     /gene="yahI"
                     /locus_tag="ACHOQQ_RS12735"
                     /old_locus_tag="pgaptmp_002547"
     CDS             complement(2806..3756)
                     /gene="yahI"
                     /locus_tag="ACHOQQ_RS12735"
                     /old_locus_tag="pgaptmp_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGU4470780.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTEVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STVLLAREIHADILVITTGVEKVCIHFGKPEQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPESLPAALRGETGTHIIKT"
     gene            complement(3806..4060)
                     /locus_tag="ACHOQQ_RS12740"
                     /old_locus_tag="pgaptmp_002548"
                     /pseudo
     CDS             complement(3806..4060)
                     /locus_tag="ACHOQQ_RS12740"
                     /old_locus_tag="pgaptmp_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4086..5504)
                     /gene="yahG"
                     /locus_tag="ACHOQQ_RS12745"
                     /old_locus_tag="pgaptmp_002549"
     CDS             complement(4086..5504)
                     /gene="yahG"
                     /locus_tag="ACHOQQ_RS12745"
                     /old_locus_tag="pgaptmp_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4470781.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFEQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(5504..7051)
                     /gene="fdrA"
                     /locus_tag="ACHOQQ_RS12750"
                     /old_locus_tag="pgaptmp_002550"
     CDS             complement(5504..7051)
                     /gene="fdrA"
                     /locus_tag="ACHOQQ_RS12750"
                     /old_locus_tag="pgaptmp_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4470782.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETSVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIDAI
                     KEAKAIAAAGGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(7041..7904)
                     /gene="yahE"
                     /locus_tag="ACHOQQ_RS12755"
                     /old_locus_tag="pgaptmp_002551"
     CDS             complement(7041..7904)
                     /gene="yahE"
                     /locus_tag="ACHOQQ_RS12755"
                     /old_locus_tag="pgaptmp_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4470783.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     HDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRFRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(7944..8549)
                     /gene="yahD"
                     /locus_tag="ACHOQQ_RS12760"
                     /old_locus_tag="pgaptmp_002552"
     CDS             complement(7944..8549)
                     /gene="yahD"
                     /locus_tag="ACHOQQ_RS12760"
                     /old_locus_tag="pgaptmp_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGU4470784.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQVLIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            8807..9304
                     /gene="yahC"
                     /locus_tag="ACHOQQ_RS12765"
                     /old_locus_tag="pgaptmp_002553"
     CDS             8807..9304
                     /gene="yahC"
                     /locus_tag="ACHOQQ_RS12765"
                     /old_locus_tag="pgaptmp_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4470785.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLCQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            9396..10328
                     /gene="yahB"
                     /locus_tag="ACHOQQ_RS12770"
                     /old_locus_tag="pgaptmp_002554"
     CDS             9396..10328
                     /gene="yahB"
                     /locus_tag="ACHOQQ_RS12770"
                     /old_locus_tag="pgaptmp_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4470786.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(10370..11458)
                     /gene="pdeL"
                     /locus_tag="ACHOQQ_RS12775"
                     /old_locus_tag="pgaptmp_002555"
     CDS             complement(10370..11458)
                     /gene="pdeL"
                     /locus_tag="ACHOQQ_RS12775"
                     /old_locus_tag="pgaptmp_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4470787.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFVSEWVMKAGG"
     gene            complement(11600..15571)
                     /gene="ehaA"
                     /locus_tag="ACHOQQ_RS12780"
                     /old_locus_tag="pgaptmp_002556"
     CDS             complement(11600..15571)
                     /gene="ehaA"
                     /locus_tag="ACHOQQ_RS12780"
                     /old_locus_tag="pgaptmp_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HGU4470788.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LALSKDVAVSEDYTAVFGKDNQTIDYRISVTDNANLVIDATDSSRPRLTLASGGGLDI
                     TGGKVHINGSLNFLLKGTGFLNVANAGSELYADDLYESYSNITHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGY
                     YDQNSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     VPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDIAQWSHNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
BASE COUNT         3606 a         4342 c         3767 g         4086 t
ORIGIN      
        1 gtgcgattat cgataacaaa acgatatttc acatcaccac gcagcattcg ctcataggct
       61 tcattaattt gatcggcccg aatcatctct atatcagcca cgatgccatg ttcggcgcaa
      121 aaatcgagca tctcctgagt ttctggaatg ccgccaatca tagaaccggc tatcgcacgg
      181 cgtttcatga tcaggttgaa aacttccggc gatttatgcg gtgtcgcagg cgcaccaacc
      241 agcgtcatgg taccatcacg cttcagcaag gtggtaaaat cgtcgagatt atgtggcgca
      301 gctactgtat tcaaaatgaa atcgaaactc ttcagatgag ccgccatctc atcggcattg
      361 cgtgagttaa caacttcatc ggcccccagg gcttttgccg cttcgcgttt tgcctcagaa
      421 gtggtaaatg ccaccacatg tgcccccatc gcgtgggcca gcttgatccc catatgtccc
      481 agaccgccga tgccgaccac gcccactttt ttacccggtc cggcctgcca gtggcgcaac
      541 ggggagtaag tagtgatccc cgcgcacagt aatggggcta ccgctgccag ttgctcttgc
      601 gggtgacgaa tacgcagaac atatcgctca tgaacgacga tctgttgtga gtagccgccc
      661 agcgtatggc ccggttcgtc cggcgtcggc gagttatagg tgccggtcat gtgatcacag
      721 tagttttcca gcccgtcttc acactcttcg caatgtttac aactgtcgac aatgcagccg
      781 acaccgacca gatcgcccgg cgcatgtttt tctacctgat caccaacggc taccacacgc
      841 cccacaattt catgacccgg cacgcaggga taaaccgtcc ccgcccactc ggaacggacc
      901 tggtggagat cggaatggca gacgccacag taagcgattt cgattttgac atcattcggt
      961 cccggttcac gccgggtgat atccatcggt tcaagtggtt gtttagcgga atatgcacca
     1021 acagctttga tcttcattgt gtttactcct gattagctat gtgtatttgg cgacgcctga
     1081 tatgtcatcg cggcaaaacg cgtccattga atatagccaa tatttaaacc acccgatcca
     1141 gccccaacat gccgcgagcc tgaatgaaac agagcagcaa cgttccccat aaggcgagcg
     1201 tctataagtg tgatatttgc tatccgtaaa agactaaaat cggcaaaact gatgatcttt
     1261 tcacgtagac aaatagttat ctacacaacg tcaataaggg catattcctc aaagataatg
     1321 tccatttctg ccgacacact catattcaga ttgccccact ccctggggct taaatctctt
     1381 actattcacc gttgcaggcg tttcaaatgc gcatcaacct gccacactcc cccacaccag
     1441 ttgcccttta tggaacgttg cggttctcgg cgagatacgc gccaccgcct cggcggaaca
     1501 ggaggcgtcc accagcacaa agctggcgtc atcctgcgct tttggccata cacgctcgcc
     1561 tttttcgttg agcggcaata catcaccagt ggctaaaaac agcgagcggg agaggttctg
     1621 ttcgttagga cgaatataga gctgcgcgta gagattggct ttttccagca tgtcgcccag
     1681 accataaggc gaccagtggt cgataacgct gtcagtgcca gtcatcactt ttacgccttt
     1741 gtcgtgcaac tgtttgagcg gcatatgcag cgtgccaatc ggcaccgtcg aggcgataga
     1801 aatttgctgc gccgccatcc gattcgccag ttcatcgacc tgttgttcgt tgagcgtagc
     1861 cagcgcaaag gcgtggctga tggtcagctt gcccttcagt tgcggcgttt tctctaccgt
     1921 ttcaaccata taattgatgg ctgccacgcc cgacggagtg gtttcgtgca ggtgaatatc
     1981 gacgcctttg tcgtagtcca gcgcaatctg gaacatggta tcgagggatt tttccatcgc
     2041 gccatcgaca ctggtcgggt ccagcccgcc gacgtaatgc gcacccgcct gcattgcttc
     2101 acgcattaag ggttccgatt tcgacagcag caaaccgtgc tgcgggaagg cgacgatttc
     2161 acactcaaag cccgcctgac gtcgcgccag caccgcctgc aaattttgca gatttttcag
     2221 gccggaaacc ggttcgatat tgcagtggct gcgggcaatg gtggtgcctt tcgactgtaa
     2281 taaatcgatc agtttttcag cccgttcctg cgtgtacggt tgcagctccg gcagcatttt
     2341 ctgctcgagt ttgatcatgt cctggatggt ggtgcctgcc gggcgattga gcgagcgcca
     2401 cggcccgccg taaaaggttt tgtcgagatg aatatgcatg tcgcgggtgg tgggcagcat
     2461 cagcttatcg ccagcgtcat agtgcggcag cgtggcatcc gggtgctgct tattctcgcg
     2521 cagcgcgaca attttgccgt tctgaatctc cacggtctgg cgcgccgtgc gggtctgcac
     2581 cgccacgcca ttttcgtagt caaatccagt ttccagcagc acgttgtcga gatagtaatg
     2641 cgggtcagtg attttcatgg tgttattcgc gtcgcagttt agggtgccag ctaccgccgc
     2701 atgggcgagc ggcacagagg taccaaacag cgcggcggcg gtgaccatct taccgctctg
     2761 gctcagaaac tcacggcggc tattgctttc tttcatctta cgtccttacg ttttaataat
     2821 atgagtgccc gtctcaccac gcagcgccgc gggcaggctt tccggcgtgg tgataatcac
     2881 ttctttgccg ccttgttcca ggaacgtcag gctggcgatg atttttggca acatgctgcc
     2941 gggcgggaaa tgtccttcct gcatatagcg agtcatggtg gcaatatcca cccgatcgag
     3001 cgcctgctgc tcgggtttgc caaaatgaat acacactttt tccacgccgg tggtgatcac
     3061 cagaatgtcg gcgtgaattt cacgggccag cagcacggta gagagatctt tgtcgataac
     3121 cgcgtccacg ctttggtaat ctcccgcgtc agtacgcacc acaggaattc cgccgccgcc
     3181 cgcgccaatc accacaaagc cttgttggat cagcgcctta atggcgggtg cttcgacaat
     3241 acgtttcggt tccggcgagg cgaccacgcg gcgatagccc cgtccggcat cttcaacaaa
     3301 acaccagtca gggtttgcct tttgtaattc gtcacgctgg ctttcactga agaatgcgcc
     3361 gatgggcttg gtgggatgag caaatcccgg atcgttttta tccacttcca cctcagtcac
     3421 cacggtgacg gcttttttct cgccgtgacg cgccagccgg ttattcagcg cctgttggat
     3481 cagatagccg atgccgccct gcgtatctgc cacacagttc gccagcggcg ttaatggcag
     3541 cccttcgcgc tcgtgggcaa tctccgcacg gcgtaaatcc agcccgacct gcggcccgtt
     3601 accgtgggtc agcacaatgt cgtaatcgga agccagcatt tccagcaccg tatcggcgac
     3661 ggctttcacc gcctccgcct gatgctcaat cgactggctg gcgttatctt tgataatgct
     3721 gttgccacca atggcaacga ccacaagctc tttcatgttg ttatgtccgc tacagagggt
     3781 gatggggtta tgaccaacgc cagactgcct gatgcgctac gcttatcagg cctacaaatt
     3841 ttcgtgccat tcgtaggccg gataaggcgt tcacgccgca tccggcaatg gtgacaaatg
     3901 cctgatgcgc tacgcttatc aggcctacaa attctcgcgc cactcgtagg ccggataagg
     3961 cgttcacgcc gcatccggca atggtgacaa atgcctgatg cgctacgctt atcaggccta
     4021 cagattctcg cgccattcgt aggccggata aggcgttcac gccgcatccg gcatgaacat
     4081 gtaggtcaga cgccgtaacg ttcgcaccag ccaagaatgg ctttctcgaa gcaggcaaaa
     4141 ggtggatgca caatgcccgc accaatcatg ccgacgcccg catctttatg ggcgatggcg
     4201 gtgttgatca ctggcagaat gccgctgcta cccacgcggg tgatgtcgat tgccgacggc
     4261 acgcccataa aaccgagcag cggaatggtg acgttggggt tttcgccaag ggtgatttcg
     4321 cgcatctgac gggagaaatc gatagcctct tccaccgtgc cacccaccag cgcgacgatt
     4381 gcaggcgcgg tcgccatggc aaatccgcca ataccgtagg tttccgtgat ggcgctgtcg
     4441 ccaatatcca gccctgaatc ttccggctta taaccggcaa acatcgggcc gatgacctgc
     4501 tgcgccgggc cggtaaacca ctgccccggc aggccgctga cccgcaggcc gaactcgacg
     4561 ccattacgcg ccatggtggt gaccacggtg ctgtattcaa tgccatgcgc cgcatccatc
     4621 gccgctttac acatcgccat ccacgtcggg ccggagaagt aatcgctact ggcaacaaac
     4681 tcaaacactt cgcgctgttg cgcgacggaa tagcccgcct ggataatccc cggcgtcagc
     4741 gcctgaatca gcagcgtcgt cccggcgtta ttgcggttat ggcactcatc gcccatatgc
     4801 agcgcctgcg ccagcattaa gcgcagatcg atttcgccga taattttcat cgcgtcgcgc
     4861 agtattggtc cctgcacatc acgcatccag ttcaggcggt caatcacgct ctggtcgtta
     4921 gcgcccatac gcaaaatctt cgccatctgc tcgctcatgt tggtataagc gatgttgccg
     4981 taggttttgt tttttacgat gtgcataaac atcgaggccg aggtaacacc cgccatcgat
     5041 cccacgcagt cgtgctcgtg acacggcgag aaagtgatct ccccggaagc cgccagttca
     5101 gccgcctcgt cgagatcttt cgccagtcct tcgaacacca gcgctccggt gaccgcgcct
     5161 ttcatcgcgc cgcacatttt ttcccaggtg atcggcggcc cggcgtgaag aatggttttc
     5221 ggcgtcatgc ccggcaccac gttaatcgcc tgctcaaaac caatcagcac cggatgcgac
     5281 tggataatgc gctccagcgc ctgctggtta gcggcggcga ttttgtctgc cagcggcgaa
     5341 tcggcaatgt tgtccagcgc ctgcaccacc tgcatattgc cctgccccgg cggcgtccag
     5401 tcgagttgag tcacttctac atgctgcttt ttcaggtcat cgctaaacat ggcgatgccg
     5461 acgttaataa cgttcaatgg ttggctaaac agtgactggc tcatcaggct tcctcccctt
     5521 tgcagataaa ttcacgcgcc agcaatccgg tattggtgct gctgctcgcc agaattaccc
     5581 cggcatcaag cagcatctgg ctttgttgtt ctaacgatgg cgtatcgaga tcggtgccca
     5641 gcacataggc gagaatgatc aactcgcgcc ctccggctgc ggcgatcgct tttgcttctt
     5701 tgatcgcatc gatggtggaa ccaaccggat cttcgtgcga tccaaatccg agcacaaaat
     5761 ccatcacgat caccgccact tctggatcgc gcgcttcttc gatcaagcga ctgatgcggt
     5821 tggtggggtc aatcattgga tgcggcttgc cgttggtgaa gtcgtcatcg ccaaagtcga
     5881 ggaaggtgtg tttgatgctg cggttgatat ctttcaggcg gaattccgga tcgggctgaa
     5941 tgttgctgta gacatcgcca tgtttttcca tcaccgcgaa catggtttcg tcgcacaacg
     6001 tgccgccgca gaacaggcca cgaatgtatt tctgctgcgg ttgcagacgc gcacgcacat
     6061 ccgcaatcaa cggctggtta agcgtatgta ggtcgagatt ttcctgtttc acgccggaga
     6121 gcatcaccgc ttttagcgcc gcttctttgc tgccgcgagc aaactgtagc ccctgctcat
     6181 ccactgacgt ttcgccacga ccgaggaagc agaccaccac cggcttgcgg caggcgcgcg
     6241 cacgttccag cactttgcgg gccaccgcag gcgcaggcgg tttggagatc agcgcaatga
     6301 tttcagtttg cggatcgttt tccagcatcc cgatggcgtc gagcatcatc aggccgccga
     6361 ttttctcgct caggtcgcgc ccgccggtgc caatcagttg tgaaacgccg ccaccaaatt
     6421 catgaatgcg gacgctcagc tcctggctgc cggtgccgga tgcgccaaca ataccgatgt
     6481 tgccgcgacg cacggcgtta ccaaaacaga gcgccgcgcc gttgataatc gccgtgccac
     6541 agtctggccc catcatcagc agcccttttt cgtgggccag ttgcttgagc gccagttcat
     6601 cttcaactga gacgttatcg gaaaacagca tcacgttgag gtcattttgc agcgcctgac
     6661 gcgcttcgcg agcggcaaac agaccgttga ccgaaatcac cgccaggtta ctttccggga
     6721 tatgcttttt ggcgctggca atagtggcgt aacgcgcctc gtgcgagccg ctttgcgctt
     6781 tgctgttgaa cagttcttca atctccacca gcaactgctc gttgtccgcc cccgatttac
     6841 cattgatgac aatcatcagg tcgccgtttt tcgcctgttc cagctccggc gtcagcagtc
     6901 ctaaattttt cagcacacct ttgttcattt cggtcgccat cgccacaaat gcctgctcga
     6961 cgccgtcgag tttatttgca cgcgtggaga tagacatcag cgagacagaa tcaaaatagg
     7021 tattcggttt aatgactatt ttaactgaca taattccctc cgtaggtttg gctcagcgca
     7081 caaccatcgg ccatgccata cagcatgtcg cagccggaac tggagccaat atgtttaatt
     7141 ttttctattg cctgagttgc gttcccaggg atgtcataaa taatgttgtg aataaaacga
     7201 tgaatatttt cccggaagcg ttgttgtaat gcggcttcca gcgttatggc acttaataac
     7261 gtggtattat ttctgccgcg ttgcaagccg agataaaatt cctctttgta ttttcccgcc
     7321 ggatgcccgg aaataaataa aataagtgcc agacctgtta aataatcatc ggctgatggc
     7381 gtaaggccaa tccctaagcc catcatactg gcgaccgctg aggcgatatt ttgtttatcg
     7441 attactgcct gtataactgc ccgtcgttta atatgtaatt ctttatttaa cgcctgataa
     7501 aacggattat cgccgcgata attaaacagg gtttcatttt cccccaggtg ctgatgaatg
     7561 atgtcgtgcc actgttgcca ggcgataagg cgaaaattca cagcggtcat ttgcaaggtt
     7621 ggggacagcc aacgccgaca acgactcatc tctatatgaa gatgttgacc aaccgtaata
     7681 ccttgatcgt caaactgaac cttatcacca tgccggaaca gatcatcaaa gtgagtgagt
     7741 gccaaccgac aactgtttgg cgcattgtcg tgctcttcac aaagcagcgt cagcaactgc
     7801 tggcgagccg ggagtgcgat atttaccgct ctggcaaaga cctgttcaac ctgtccttgc
     7861 ccccgctgcg ccagaaaatc cgcatccgca gttagcgccc acacacaccg tttttctggt
     7921 gtgttgatag caagcctccc ggtttatgca cctgcggcaa tcagtaactg cgcaatctct
     7981 tcaaagcccc gttcccgcgc cagttccagc ggcgttttgc catatttatc cgtcatgtgc
     8041 gggctggccc cgtgttccag caataactgg acaatcgcct gctgtttaat accaccatca
     8101 ttaagcacaa tcgcttccag tagcggcgtc cagcctacat ggttggtctg gttaacgtta
     8161 atctccgtat gtgccaaaag ttcttttaca atacttaaat ggcctttttc acaggcaggc
     8221 gtcaggccga caccgccaaa acgggttacg caattaagat cgggtttagc cggtaaaata
     8281 attcgtagta gcgttaaatc atcgttcaga cagctaatta aaaaaggatt taaacaggta
     8341 tgatcttgtt tattaatatc cgctccggcg tcaattaata cctgaacgca agcatattgc
     8401 tgatataaac ttgccagcgt aattgccgtt ttcccctgac gatcgcaggt attaatatcg
     8461 acacccagcg caaggcaggt ttttacttta tcgatatcac cctgttgtgc agccaataaa
     8521 tagtcggcag gtagattttt tatactcatt cacattcccc tggtttttgt ggctgttttc
     8581 gatgaattga gaatagcaac gataatctga taaaagaacc cgcttaaatt taattcgccg
     8641 tatttcgcct ttttgttgaa tgatgttcaa caatcttttc gcagtgtgcg atagtgcaat
     8701 atttatttct ttgacccgat accctttcac tcaatcaggt tttatttctc tatagtctgc
     8761 acatataagc gcagctttcg tccatgtcta accagagggg aacaagatga acggactcac
     8821 ggcaacgggt gtcaccgtcg gtatttgtgc agggctttgg cagttagtgt cttcacatgt
     8881 cgggctctgt cagggatggg aattattagg aaccattggc ttcgtcgcct tttgcagttt
     8941 ttatgccgca ggcggcggta aatcggggtt tatcagaagc ctggcggtta attattccgg
     9001 gatggtgtgg gcgtttttcg ccgcgctggc tgccggttgg ctggcctctg taagtggact
     9061 gtccgcgttc tgggcgagcg taattactac cgtacccttc tcggcggtgg tggtctggca
     9121 agggcgtttc tggttactgt catttattcc cggtggcttt ctcggcatga cgctgttttt
     9181 tgccagcggg atgaactgga cggtgacgtt actgggtttt ctggcgggta actgcgtggg
     9241 gataatttca gaatatggtg gacaaaaatt aagcgaagcg acgactaaac gcgacgggta
     9301 ttaaaagtta ttcaaaaatg attatctatc tttaaccgat acctctacag tcatagctgg
     9361 agaaattcat agtagataat ggcgggaatg gtttaatgaa ctcaattttt accgaagaga
     9421 atctgttggc atttactacc gctgcgcgtt ttggcagttt cagtaaagcc gcagaagagt
     9481 tgggtttaac cacttccgcc attagctaca ccattaagcg tatggagacg gggctggatg
     9541 tggtgctgtt cactcgcagt acccgcagca ttgagttaac ggagtccggg cgttattttt
     9601 tccgcaaagc taccgacctg ctgaatgatt ttcacgccat caaacgcagt atcgatacta
     9661 tttcgcaggg catagaagcg cgtgtgcgca tttgtattaa tcagctgctt tatacgccca
     9721 aacacaccgc gcgcttatta caggtgctga aaaagcagtt tcctacctgc cagattaccg
     9781 tgacgaccga agtgtataac ggcgtctggg atgcgattat taataatcag gccaatatcg
     9841 ccatcggcgc acccgataca ctactggacg gcggcggtat tgattacact gaaattggcg
     9901 cgattcgctg ggcttttgct atcgccccgg atcacccgtt agcctttgtg ccggaaccca
     9961 tcgccgaaag ccaactgcgt ctctacccta atattatggt ggaggacacc gcgcatacga
    10021 ttaataaaaa agtgggctgg ttgctgcacg ggcaggagtc aattctggtg ccagatttca
    10081 acaccaaatg tcagtgccag attttaggtg aagggattgg ttttttgccg gattacatgg
    10141 tccgtgaggc gatgacgcaa tccctgctgg ttacccgaca aatccataat ccgcgccagg
    10201 attcgcgcat gttactggcg actcagcatt ctgctacggg tcaggtgacg cagtggatta
    10261 aaaagcaatt tgcgccaaat ggcatattaa cggggatcta tcaggattta ctgcatcggg
    10321 agaattaatc gctgccaacc tcccgatgcg ctgtgaatag cggtttacat caaccaccag
    10381 ctttcattac ccattcagaa acaaatttat tacccggtac tggcggagag tacaaatagc
    10441 cctgcaaaaa gtgaacgcct ttaccgatca ttaaatccgc ctgctcctgg gtttctaccc
    10501 cttccgccac gatactcaga ccaggcttac gcgccagttc gacaatattg tccacaatat
    10561 gaccggatat ttcgtcgaca ctcgccattt gcacaaatga cttatcgatc ttaataaaat
    10621 cgaccggaaa cgcctgcaga taacgatagg tcgcatatcc cgtaccaaag tcatccagcg
    10681 caaaggtaat gttatgctga tgaaggctgt caaatatcgc tctggcttct ggcgttaccg
    10741 gaatagggtt acgttccgtt agctcgagaa ccagtttgat tttatcgttt cctaatttct
    10801 tgaccaggtt caggcattct ttttcaaatc ccgcggccaa aaaacaaccc gccgagacgt
    10861 tgataccaat atggaaatta tccggcagca aatgttttac cggcatcaga atatccgcag
    10921 tctgtttcat cagttggcgg gtcattatga caataagacc ggatgactcc gccagaggaa
    10981 taaactgatc cggcgggatg attcccgttt gtggatgttc ccagcggaca agcacctcac
    11041 agcccgtcag taccccagtc tgcgcgcaga aaaccggttg gatccacggt ttgaattcgt
    11101 ggttttccag cgccagactg atggcttcag gcgtgacgat atggtgacaa cggttatcag
    11161 ggatataaaa attattttta tttcccgtgc cactgatgac ttgcggatga tattgaaaaa
    11221 agatatcccg ccagaaggta atatcactct ggatgcctag tttttcatac agctgcttct
    11281 tttgatgtga aattgtcttt gcactgcgat ttctgtactg tgagatttct gtaaccgaca
    11341 ttccctccat tagcagcttg agtaaaagcc gttctttttc ggacacgata ccaggcaacg
    11401 tcaaattcac cgttgccccg aactcttcca aaaaaacact caaatcatgc gaagccattg
    11461 aacacacctt gattaaatct tgtgcgccag ctaaatctgc gagtcttcac tctgacggat
    11521 aaagctggct gcaagaaaat gacacagaga gccgaattat ctcccgttcg gctctctctc
    11581 cttttactca ttattcagat cagaattgcc acttaatgcc aaccattgca gaggtgtcat
    11641 tatatcccct atcggcaacc tgaacgccca cattccccca gacattcagg ttggcattta
    11701 gctgtccttc caccccggtt tttatctcag caatatttcg ggctccatcc tgagtgacgg
    11761 acacgccatc catactggtg ctgaaatcct tactgttatg cagccagttc acttctacaa
    11821 acggctggaa ctcgcgggat ttaccgtcat ccattttatg gtggctcttc agccaggttt
    11881 ttacgccaag tcgggtttga acattgccat caccgttgct atgaacgagg gttccgttgc
    11941 tttcgcggtg cttatcggct ttgactccca tccaggtaac ctgtgcttgc ggctgaacat
    12001 accattcatt acgcgttccc tggctgccat taaattcagc taatttgtgt ttgtatccag
    12061 cttccagtga agcggtaaat ccttttgatt tataggattc actttgtaag tcatcccctt
    12121 tcactgtgtt atcaaaccag ctgtactgcg cccaactgtc gagatacgcg ccattacgcg
    12181 actcgtcatc ggcataccag gtggcataga ggcctgtgct atatccgttc acactcgctt
    12241 ttgcacgata accggttcgc gaggaaatgg ttttgctgtc gctgttgcca tatcccgcca
    12301 tgaccccaac atgccagctg tcgctgccgt tatggctcca ctgcgcgata tcgcctccca
    12361 gttgcagaac atagcgattg ctttgggttt tcagctggcc gctgccatca cgccatttat
    12421 tatgaccacc ttcatggcgc atccacatag tggtttgttt ctgctcaccc gtcaccatgt
    12481 cggtatagta cgtattaccc agacgctcat gcagacgcgt ggtgaacatg gtattcgctg
    12541 ctgcaaggtt cgcaatgtag ctacccgcct ccggtcgcag gtcattatcc acattcagat
    12601 ccgggcccgg cgtaggtgtc gggttagggt tcggctctgg attcggattc ggcaccgggt
    12661 ctggctccgg ctgcagttca ggagaatcac tgccgctggt cagataccag ttaccactat
    12721 ttgccccttg tccacgcgcg agagtgtagt catacgcccc cgcaacgata cgaccagcct
    12781 gaacaaattc gccctcagac ttaccgtcta catggataag ttcgataccg ttaagggttt
    12841 tcgcacctgt accgccagcg ttattcaccg taacggcagt cgtgccgcta gtatcgccct
    12901 cgacaaccaa tttatcggtt actgagtcat cgccattcag taccgtattc attactagtt
    12961 gaccgttatt accgtggtag ttccccttca ccactaacac attgccagct tgctgtcctg
    13021 acattccggt catcagcgta ccgttgttgg tcagattgcc gcccagggtg taattcacag
    13081 acgcagccgc tcttttttca gcgctattat tcaacgccag ggtaccgttg ttctcaacgt
    13141 ttgcgctacc caatccctgc gcatgactta ccaggacatt agccgaatta gcaatgttca
    13201 cattcgccgt caggttgctg ttatcgcccg cgatgtccag cgtgccgccc tgaatattca
    13261 gctctccgct ccccgttaac gaaccggttg aagtgccccc gttggtaacc gtcagactgc
    13321 cgccatttaa gctcagcagt gaatcggcgc tgccattgag cgtgcccacg gtctggctgt
    13381 gaccattcat atccagttca gtctctgccg ccagacgcag ttcatgggta ttacccaaca
    13441 cgttatcgtt attcaacaaa agcgtcccac tgcgcagatc ggtaacaccg gtatagtcgt
    13501 tgtctttgtt agaaagcgat acggtctgac ccttctggct gctgaatgcc aggtcaccac
    13561 tgccggtaat ctttgcgctg agatcggcgg cattctcgct tttaccgtta gagctcagca
    13621 ccagagcatc gctgtcggta gcgtgtaaat caagctgggt caggccgtaa ccgatataca
    13681 gaccgtcatt gtttacccca ctcgtcagac gatagtcgta agtgccgacg gccgcttttt
    13741 gcccattctg ctgaatatca cgttgggcgc tgctggagag gatttgccca tttttatcct
    13801 gcaattgcag attgcccgca tcgcccagaa cttccgcacc ttgcgcatca accaacttaa
    13861 tggtgctgtt accatcatcg acttcagtga gcgataacgc agagtcaata tcacgagata
    13921 ctgagcttac aacgtcactg tcagaaacct gaatcacacc ctcgccgcgc aaatccagcg
    13981 ttttactaac gttgaccgtc ccttctgtca tctgtgcacc tgctgtcagg gcaccaaaat
    14041 caacggttcc gccggagaat cccaaagtgc taatggagtg agcgccctta cccactgtta
    14101 ccaggcttcc ctgacccgca gtgatcccgc ctttctgcag aaactcagca ttcttttcca
    14161 gattaaatgt cgtgttctgg aacgccagtg aaccctggaa ccagtctgta taagcaaact
    14221 catcgaagga gaattcgtga ttccccatgt ttatttcaac aatacctcta ccttcaagat
    14281 cgttatcaaa tacccaatct ttattggcat tgatttgcag tttgccatca acatccaggt
    14341 ccgcacggcc gatgttcttg tcttcagaaa taaccgcgac agcaccagaa gcgacatcaa
    14401 tatatccgct gaatgcatag ttattgccgg tcagtgccac tgtcccactg tcgatactta
    14461 ttgtaccatt gccggaaatc ttattggcaa aggtcatgct atcgtcagcc ttcaggtgta
    14521 atttcccgcg gttgttgatt tctgctgtac ctatattttg ggtgatccct aaagcgccat
    14581 tttgctcaat tttgacttta ccgctaaagg cggagttatc cccggttaag taagtcgtac
    14641 cattgagcgc attgatatag cccaggccct cgctgtcact gacaatatcc gcggaaatag
    14701 tcgcgttttt atccgtgtgg tttaaggtga ttttcttctc ggatgtcttt gcccacacaa
    14761 actcaatttt ttcggcatca ataatccctg cagccttcgc tgcgcttcct tcctgtgcac
    14821 ctaacgctaa ctcagagttg gtgcttaaac taatagtcgg cgcagagatt ttaccacctt
    14881 ccgaaacggc aagtgttgta tcggagtttt gatcataata acccaggcta attttctcac
    14941 tggcattaac ttcaccgccg ttattaactg acaggtactg attaccaccg taactgccgt
    15001 aaacgcccat aaagaaggtt ttagaattta cctggctgcc ttcaccgctg acatttccct
    15061 gcaaataggt cagacggctg gtgcccttaa catggatttt gccgccgttg gagacattaa
    15121 aatagcctct atcgtgggta atattagagt aagactcata taaatcatca gcatataact
    15181 cgctgccagc attggcgacg ttcagaaacc ccgtcccttt taacaaaaag ttaagcgagc
    15241 cattgatatg caccttccct ccagtgatat ccagcccacc accagacgcg agagtcagac
    15301 gtggacgaga agaatctgtc gcatcgatta ccagattggc attgtcggtc actgaaatac
    15361 ggtaatcaat agtctggtta tctttaccga atacagctgt gtaatcttca cttacagcga
    15421 catctttaga cagtgccaga gatgaatata aacttatcgc gctaattaaa acagcccggg
    15481 acttttttcc ttttactctg ctccccagct cagagcagac gctccaacag ttacgggacc
    15541 tgttccactt tagccgatat attttattca tcacaatctc cagcaataca tccaaataaa
    15601 accaagattg cataaacagc aacgcattaa atgccattcc ggttactgtt catcgcatat
    15661 aaaaattcaa aaattttgca tagcattggc aaccacatat ctgagtatgt agttcagagg
    15721 gtaataaaat agcaagttac tactgaaaac ttattttaag ttaacactct taactttatc
    15781 attacattcc ccccccagtc t
//
LOCUS       DAZKFU020000101        15649 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZKFU020000101 DAZKFU020000000
VERSION     DAZKFU020000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.102"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            262..468
                     /locus_tag="ACHOQQ_RS12785"
                     /old_locus_tag="pgaptmp_002557"
     CDS             262..468
                     /locus_tag="ACHOQQ_RS12785"
                     /old_locus_tag="pgaptmp_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470789.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            1021..1515
                     /locus_tag="ACHOQQ_RS12790"
                     /old_locus_tag="pgaptmp_002558"
     CDS             1021..1515
                     /locus_tag="ACHOQQ_RS12790"
                     /old_locus_tag="pgaptmp_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGU4470790.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1515..3617
                     /locus_tag="ACHOQQ_RS12795"
                     /old_locus_tag="pgaptmp_002559"
     CDS             1515..3617
                     /locus_tag="ACHOQQ_RS12795"
                     /old_locus_tag="pgaptmp_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4470791.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSHSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            3614..3826
                     /locus_tag="ACHOQQ_RS12800"
                     /old_locus_tag="pgaptmp_002560"
     CDS             3614..3826
                     /locus_tag="ACHOQQ_RS12800"
                     /old_locus_tag="pgaptmp_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470792.1"
                     /translation="MNQNDIEAMIQRYMEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            3826..5334
                     /locus_tag="ACHOQQ_RS12805"
                     /old_locus_tag="pgaptmp_002561"
     CDS             3826..5334
                     /locus_tag="ACHOQQ_RS12805"
                     /old_locus_tag="pgaptmp_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4470793.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFVQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKDYEDYELTAARIAAALGMYIRKG
                     DGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5279..7306
                     /locus_tag="ACHOQQ_RS12810"
                     /old_locus_tag="pgaptmp_002562"
     CDS             5279..7306
                     /locus_tag="ACHOQQ_RS12810"
                     /old_locus_tag="pgaptmp_002562"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU4470794.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7393..7716
                     /locus_tag="ACHOQQ_RS12815"
                     /old_locus_tag="pgaptmp_002563"
     CDS             7393..7716
                     /locus_tag="ACHOQQ_RS12815"
                     /old_locus_tag="pgaptmp_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HGU4470795.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGLTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            7709..7984
                     /locus_tag="ACHOQQ_RS12820"
                     /old_locus_tag="pgaptmp_002564"
     CDS             7709..7984
                     /locus_tag="ACHOQQ_RS12820"
                     /old_locus_tag="pgaptmp_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU4470796.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQHWTVTRHERFNGKPMIFIE"
     gene            7996..8574
                     /locus_tag="ACHOQQ_RS12825"
                     /old_locus_tag="pgaptmp_002565"
     CDS             7996..8574
                     /locus_tag="ACHOQQ_RS12825"
                     /old_locus_tag="pgaptmp_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4470797.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLTRR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLVNGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            8571..8972
                     /locus_tag="ACHOQQ_RS12830"
                     /old_locus_tag="pgaptmp_002566"
     CDS             8571..8972
                     /locus_tag="ACHOQQ_RS12830"
                     /old_locus_tag="pgaptmp_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4470798.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8983..9726
                     /locus_tag="ACHOQQ_RS12835"
                     /old_locus_tag="pgaptmp_002567"
     CDS             8983..9726
                     /locus_tag="ACHOQQ_RS12835"
                     /old_locus_tag="pgaptmp_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4470799.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEICAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASINVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            9787..10173
                     /locus_tag="ACHOQQ_RS12840"
                     /old_locus_tag="pgaptmp_002568"
     CDS             9787..10173
                     /locus_tag="ACHOQQ_RS12840"
                     /old_locus_tag="pgaptmp_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4470800.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAGEG
                     ELSEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAGDDEHEALTPEKS"
     gene            10182..10511
                     /locus_tag="ACHOQQ_RS12845"
                     /old_locus_tag="pgaptmp_002569"
     CDS             10182..10511
                     /locus_tag="ACHOQQ_RS12845"
                     /old_locus_tag="pgaptmp_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4470801.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10483..13548
                     /locus_tag="ACHOQQ_RS12850"
                     /old_locus_tag="pgaptmp_002570"
     CDS             10483..13548
                     /locus_tag="ACHOQQ_RS12850"
                     /old_locus_tag="pgaptmp_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4470802.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKSAQAHERMAREVEKTRQRMEALSQK
                     MRDEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQISALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNELQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSSGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSYMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13548..13877
                     /locus_tag="ACHOQQ_RS12855"
                     /old_locus_tag="pgaptmp_002571"
     CDS             13548..13877
                     /locus_tag="ACHOQQ_RS12855"
                     /old_locus_tag="pgaptmp_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4470803.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            13887..14585
                     /locus_tag="ACHOQQ_RS12860"
                     /old_locus_tag="pgaptmp_002572"
     CDS             13887..14585
                     /locus_tag="ACHOQQ_RS12860"
                     /old_locus_tag="pgaptmp_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4470804.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSTSFVLSTPTETDG
                     SVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14590..15333
                     /locus_tag="ACHOQQ_RS12865"
                     /old_locus_tag="pgaptmp_002573"
     CDS             14590..15333
                     /locus_tag="ACHOQQ_RS12865"
                     /old_locus_tag="pgaptmp_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4470805.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            15231..>15649
                     /locus_tag="ACHOQQ_RS12870"
                     /old_locus_tag="pgaptmp_002574"
     CDS             15231..>15649
                     /locus_tag="ACHOQQ_RS12870"
                     /old_locus_tag="pgaptmp_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4470806.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPSFRQKLNEGWYQVRISGRDAGENELSARLNEPLANGAVIHVVPRLAGAKSGGV
                     FQAVLGAALIAVAWWNPVGWLGAAAVSGLYAAGASMIL"
BASE COUNT         3761 a         3640 c         4718 g         3530 t
ORIGIN      
        1 atgctattca acaatacaaa ttacccataa acctcgtttt tacggggttt tgttatattt
       61 aaacattacc gaatagatat aaatctcgat tgtcgatggt attggttgtg tgacaatacc
      121 tagtctttcc ggtatgcctg aagagaatac aaacgacaga ttatgtaagg gaaacgcgct
      181 gaaactttca ctctttcatg ctgagtgatt tgacgttata tttttagccg caagtgaaga
      241 gcaaacgcat ggagcgacaa aatgcataaa gatcaataca ctgatgcccc ctcccaggag
      301 caggttcgtg taaaaactat gcttaatagc accatttcga tgggttaccc ggatgttgta
      361 attgcatgta tagaacatca agtatctctg gaagcattca gggcaattga ggccgcgtta
      421 gtaaagcacg ataagaatac gaaggattat tccctggtgg ttgactgatc accataactg
      481 ctaaccattc tgaccatttc acaagtgaca gagtcaatat cgtattctgt cactgtcagg
      541 ctaatacagt actgcaattc aactacagca atgcctcgta tttaggtgaa tttacaatat
      601 cgtccagttc ggatgccggc tgcatttctg aagataaggc atttcggttc gcgtattttc
      661 ccctcatgct cgtcagtcct gcgcgtagta agaaacagga cactcacact aatttgtgtg
      721 ggcatgctgt gatgtccttc tgaattatcc ccacgccatt atgtaaagct ctgtttcaga
      781 tgctcgtcac ggttgtcagg ctgtcgggtc ctccctgtgg ggggccctgc cacggggcgg
      841 gagcgtcgcg gaaaaaggct agtttttgaa atttcattcg tcatcaccac cactgtaata
      901 gattgatatt acagtggttt tatttttatg gtgtcgattt tgattgtttt ttgttcatca
      961 ctaacaccgt ttgcctaaag ttgttcgcaa gatgcatgtt taaaacattc tggagcgggt
     1021 atggatcgag agttaaaaaa tctgacgctg aatatcagtc aactggcggc actgtcaggt
     1081 gtacatcgcc agactgctgc ggcaaggctg caaaatctac ccgttgcagg ggggcatgaa
     1141 agcaacctca agctttatcg ggtggttgat attgtgtcgg catttctggc attaccaccg
     1201 ccggttgcag aaggcgaaat ggacgcgcat gagcgcaaag cctggtatca gtctgaacgt
     1261 gagcgtctta agttcgaaca ggaaacggca caactcattc cggccagtga tgtcagacgg
     1321 gagtttgcca tctgggcaaa agcggtcgtg caggtgctgg agacattacc ggatattctt
     1381 gaacgtgact gcggtctgca gcctgccgct gtgagccgtg ttcagtccat tattgatgat
     1441 ctgcgcgatc agatagccct gcgggtgact gaagcaggtg cggatgatga ggaggaatta
     1501 cagcaggagg agtaatgctg aatcaggaaa ccgcaaaggc agcacgaacc gattcaggtt
     1561 atatccttcg cgcaccgaga cgaatgcggg ttgctgatgc cgttgctcag tatatgcggg
     1621 tgcccatggg ggcagggaac tcagtcccgt gggatccgct ggtggcaccg tatgttattg
     1681 agccgatgaa ctgcctggcc tcgcgtgaat acgacgcagt gatatttgtt ggcccggcac
     1741 gaaccggcaa gactatcggc ctgattgacg gctgggtgat ttacaacgtg atttgcgatc
     1801 ctgctgatat gctgatcatt cagatgacgg aggaaaaagc ccgcgaacac tccaaaaaac
     1861 gactcgccag aacgtttcgc gtcagcccgg aagtggtcag tcgcctgagt ccgaacaaaa
     1921 atgacaacaa cgtttatgac agaacattcc ttgctggtaa ctacctgaaa atcggctggc
     1981 cgtcagtcaa tatcatgtcc tcatcagatt ataaatgcgt cgcgctgacg gattatgacc
     2041 gttttccgga agatattgat ggcgaggggg atgctttctc tcttgcctca aaacgtacca
     2101 caacatttat gtccagtggt atgacgcttg tggagagttc ccccggcagg gatgtgaagg
     2161 atgtgaaatg gcgacggact tcaccgcatg aggctccacc aaccacgggg atactgtcgc
     2221 tctataaccg tggcgatcgc cgtcgctggt actggccctg tccacactgt ggtgagtatt
     2281 ttcagccctg cggcgatgtg gttgctggtt tccgtgatat tgccgatccc gtgctggcaa
     2341 gtgaggcggc ttatattcag tgtccttcct gttcaggacg gattatgcct gaacaaaaac
     2401 gtgagctgaa cggacgtggg gtctggttgc gggatggtga atccatcaat gcggatggca
     2461 gtcgttatgg tgatccccga cgctcacgta ttgcgtcatt ctggatggag ggtccggcag
     2521 ctgcttacca gacactctcg caactcgttt acaaactgct tactgcagaa caggaatacg
     2581 agacaaccgg aagtgaagaa acactcaaga cggttatcaa taccgactgg ggattacctt
     2641 atcttccccg cgccagcatg gagcaacgaa aaagtgaact gcttgagcag cgggcagagc
     2701 cagttccttc ccacagtgtg ccggatggcg ttaatttcct tgtggcgaca gtggatgtgc
     2761 aggcgggacg tcatcgccgt tttgtggttc aggtaacggg ctatggcagc cgtggcgaac
     2821 gctggattat tgatcgttac aacatcacgc agtcattgcg cggtgacagc gacggggaga
     2881 gccagcgaat tgatccggcc agctatccgg aagactggga tgtcctgctg acggatgttt
     2941 ttcataaaag ctggccgctg gcctccgatc cttctcaaca aatgcgactg atggcaatgg
     3001 cggtggactc cggcggtgaa gacggggtca ctgataatgc ctataaattc tggcgtcgtt
     3061 gccgtcgtga tggccttggt aaacgtattt acctgtttaa gggcgacagc atccggcgcg
     3121 caaaactgat cactcgtaca ttccctgata acaccggacg aacgggccga cgggcgcagg
     3181 ccgcaggtga tgtgccgctc tggcttcttc agacggatgc actgaaagac cgggtgaata
     3241 acgcgttatg gcgtgactcg ccaggtcccg gctatgtgca tttccctgac tggctgggga
     3301 gctggtttta cgacgaactg acgtatgaag agcggagcag tgacgggaaa tggagtaagc
     3361 ctggtcgcgg tgccaacgaa gcttttgacc tgatggtgta tgccgaggct ctggtcattc
     3421 tgcatggata cgaaaagatc cgctggccgg atgcaccgga gtgggcgagc cgggaaacct
     3481 ggctggagtg tgtccaggac agtaccgaac cgtcatcctc accggaaccg gtatccacgc
     3541 ctgttaaaaa acaaaaacgg aagaaaacag taactgacga tgttaacccc tggctgactt
     3601 ccggaggatg gttatgaacc agaatgatat cgaagccatg attcagcgtt atatggaagc
     3661 tgaaatggcg gtgctggacg gaaaatccgt cacctttaat ggtcagcaga tgaccatgga
     3721 aaacttatct gagatccggc agggacggca ggagtgggag cgccgccttg cggctctgat
     3781 tacacgacga cgggggcatc ccgggtaccg gctggcgagg ttctgatggc aattcttgat
     3841 gatgtgattg gcgttttttc acctggatgg aaagcggcaa ggctgcgttc ccgtgcggtg
     3901 atccaggctt atgaagccgt aaaaacgacg cggacacaca aagcccgacg ggagaaccga
     3961 actgccgacc agctaagcca gtacggggcc gtgtcgttac gtgagcaggc ccgttacctt
     4021 gataacaacc acgatctggt tattggtgta tttgacaagc tggaagaacg ggtggtgggg
     4081 aaaaacggga ttattgtcga gccacatccg gtattacgca atggggccat tgcccgtgat
     4141 ctggcagcgg agattcgcac ccgatggagt gaatggtctg tcagcccgga agtcaccggg
     4201 cagtttaccc gtccgatgct ggaacgtctg atgctgcgta cctggctgcg cgatggtgag
     4261 gtgtttgtcc agatggtttc cgggcgcata aacagcctga cgccttctgc cggtgttcat
     4321 ttctggctgg aggcgctcga gccagacttt attcccatga ccagtgatga gagcaacagg
     4381 ctgaatcagg gcgtgtttgt tgatgactgg gggcgtcccg aaaaatatct ggtgtataaa
     4441 agccgtcccg tatccggacg gcagatggaa accaaagaag tggatgcaga gcgaatgctg
     4501 catcttaaat ttgttcgccg tctgcaccag atgcgcggga cgtctttgtt gtccggtgtg
     4561 ctgatccgcc tcagtgccct gaaagattat gaagattatg agctgactgc agcaaggatc
     4621 gccgctgctc tggggatgta catccgcaaa ggcgacgggc agagctatga accggatggt
     4681 aatggcagca aggataagga acgcgagctt accattcagc caggcattat ttacgacgat
     4741 ctgaaacccg gcgaagaaat cggaatggtg aagtcggatc gccccaatcc taaccttgaa
     4801 acttttcgta atggtcagtt gcgtgccgtg gcggcgggca gtcgtctgag tttttccagt
     4861 acagcgcgca actataacgg cacttacagc gcccagcgtc aggagctggt tgaatctact
     4921 gatggctacc tgatcctgca ggactggttt attggtgccg tcacccgccc gatgtatcgt
     4981 gcatggctga aacaggctgt ggcatccggt gttatcaggc taccccgcga tcttgaccgt
     5041 tcttcactgt ataccgcggt gtattccgga ccagtgatgc cgtggattga ccctgttaag
     5101 gaggctgagg cctggaaaat ccagattcgt ggtggagcgg cgacagaatc agactgggta
     5161 cgtgctggtg gtcgtaatcc ggatgatgtc aaacgtcggc gcaaggccga aattgatgaa
     5221 aaccgcaagc tggatctggt atttgatacc gatccggcca gtgataaagg aggcagcagt
     5281 gccgcaacga aacgacagga gccgcagcac accgacgacc agtccgaaga ataattcctg
     5341 gttcaggatg caggctggtc accagagtga cgcggatatt tatatttatg acgagattgg
     5401 tttctggggt gttacagcga agcagtttat cagtgatctg aatgcactgg gcgatatcac
     5461 ccacattaat ctccatatta attcaccggg tggcgatgtc tttgaaggca tcgccatttt
     5521 taatgcgctg aaaacacatg gtgcgtccat taccgtttat gtcgacggtg tggcggcgtc
     5581 aatggcgtcg gtcattgcga tggtgggaaa cccggtcatt atgccggaaa acaccttcat
     5641 gatgattcat aaaccatttg gctttacggg cggtgatgcg gaggacatgc gcacctatgc
     5701 cgacctgctc gataaggttg aggcggttct gttacccgct tatgcacaga aaaccgggaa
     5761 aaccaccgat gaaattgctg ccatgctggc ggatgagacc tggatgtccg gtgccgaatg
     5821 tctggcacag ggatttgctg atcaggtgac gccagccgtt aaggcaatgg catgtattca
     5881 gtcaaaacgt acagaggaat ttaaaaagat gccggaatcc attcgaaaca tgattactcc
     5941 gccacgcaac agtgctccac gcgtacagga tgatgaacct gcagcctccc ggacgccagt
     6001 gcaggcagca gcacccgtgg tggatgaaaa cagtatccgt gcgcaggtac tggcagagca
     6061 aaaagcgcgt gtaaacggta ttaatgatct gtttgccatg tttggcgggc gttatcagac
     6121 gctgcaggct cagtgtcttg ccgatcctga atgttcgctg gagcaggccc gcgaaaagct
     6181 gttgaacgag atggggcgcg agtccacgcc atccaataaa aataccccgg ctcatattta
     6241 tgccggaaac ggtaattttg tgggggacgg gatccgccag gcgctgatgg cgcgtgccgg
     6301 atttgaaaaa accgaacgtg ataatgtcta caacgggatg accctgcgtg aatatgcccg
     6361 tatgtcactg actgaacggg gtattggggt ttccagttat aacccgatgc aaatggtcgg
     6421 tgcggcgttc acacacagta cgtctgactt cggtaatatt ctgctggatg ttgcgaacaa
     6481 agccattctg cagggctggg aagatgcccc tgaaacctat gaacagtgga cgcggaaagg
     6541 tcagttgtct gattttaaaa ttgcccatcg tgtgggtatg gggggcttca gtgctctgcg
     6601 tcaggtgcgt gaaggggcgg aatataaata cgtcaccacc ggagataaac aggccactat
     6661 tgcactggcg acctatggcg agctgttcag tatcacccgt caggccatta tcaatgatga
     6721 tctgaatatg ctgaccgatg tcccgatgaa actgggccgt gcggcgaaat ccactattgc
     6781 cgatctggtt tatgccattc tgacgtctaa cccgaaaatc tccacagata atgtaagtct
     6841 gttcgataaa gcgaaacatg caaacgtact ggagagcgct gcaatggacg tggcatcgct
     6901 ggataaagcc cgccagttga tgcgcgttca gaaagagggg gagcgtcatc tgaatattcg
     6961 tcctgcgttc gtactggtac cgacggcgat ggagtctgtt gctaaccagg tcattcgctc
     7021 ctcaagtgtc aagggggctg acattaacgc cggtattatt aacccggtga aagattttgc
     7081 gaccgttatt gcagagcctc gtcttgatga taacagccag accaccttct acctggctgc
     7141 gtcaaaaggc tccgatacga ttgaagtggc ttatctcaac ggtgtggata cgccatatat
     7201 tgatcagatg gagggcttca gtgtggatgg cgtgacaacg aaagtgcgta ttgacgccgg
     7261 tgtcgcgcca gttgatcacc gcggtctggt gaaatgtacg gcgtaaacgt cgcagacaac
     7321 aactctgatg gcccgtaagg gctttttttg tacctgaaat cagcccctga acgaggctgt
     7381 gcggagacag ttatggcaaa gaattttgta gaagaaggaa aaacggtggc gattgttgcc
     7441 agtgcagcca tcagcagcgg agacctggtg caggtgggtg atgtttttgc ggtggcgctg
     7501 accgatattc cacagggtga aacaggcgac ggcctgaccg aaggtgtgtt tatgctgcct
     7561 aagctgaaaa cggatgacat gaaaacgggt aagaaggttt atctgaagtc cggaaaagtt
     7621 cagttgacta acagcggctc tgatccgctg gtcggggttg tctgggcaga tgccggaacc
     7681 agtgcagaag aagtgccggt aaaactcaat gtctgatccc ttttcccggc tggcagcgcg
     7741 tatggatgcg atcacggtca gaaagatggg aaagacagcc tcgattaatg atgtcgatat
     7801 gactgtgatc ccgggagaaa cactggcaga gctgaatgct ctgtccggac ctgcggtctc
     7861 tctggtggtg ttttcttcgg gataccgccc acggcgcggg gatcgcgttg tttatgacgg
     7921 acaacattgg acggtcacac ggcatgaacg ctttaacggt aagccaatga tctttattga
     7981 gtaaagaggt gtgggatgaa ggggcttgag aatgccatcc gcaatctgaa cagccttgat
     8041 acccgtatgg tgccacaggc cagcgcatgg gcgataaacc gtgtggcaca gaaagcggtc
     8101 tcggttgcca cccggcaggt tgccgggaat accgttgcgg gagataacca ggtgaaaggg
     8161 atacccctga aactggtacg tcagcgtgtc cgggtgttta aagccagtcc gtcaggaaaa
     8221 atgacggcca ggatccgcgt taaccggggc aatctgcctg ccattaagct ggggaccgcc
     8281 cgggtcagac tgacccggcg tggtggaaaa ctgcagtacc gtggcagcgt gctgaaggtg
     8341 ggtaaatatc ttttccggga tgcgtttatt cagcaactgg tgaatggtcg ctggcatgtg
     8401 atgcggcgta ttgatggcaa aaatcgttac cccattgatg tggtgaaaat ccctctgtcc
     8461 ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc ggaaatgccg
     8521 aaacagctgg ggtatgcact gaaacaacaa ctgaggttat ggctgacccg atgaaccgac
     8581 atacacaaat ccgccaggtc gtactggcac gccttcggga acagtgtgga gacagcgcca
     8641 cgttttttga cggacttccg gcatttgttg atgcgcagga actgcctgcc gtggcggtgt
     8701 ggctgagtga tgctcagtac accggaaaaa tgacggatga agatgactgg caggctgttc
     8761 tgcatattgc tgtcttcatc cgggcacagg caccggattc agagctggat atgtggatgg
     8821 agagcaccat tttcccggcc ctgaatgatg taccggcact ttccggactc atcgacaccc
     8881 tgatcccact cggttttaac tatcaacgtg ataatgagat ggccacctgg gcgatggcgg
     8941 aaatcacgta ccagatcacg tacacgaatt aaggaggtgg taatgaccac accaaatcca
     9001 ctggcaaaaa cgaaaggtgc gggaacgacg ttctggatgt acaccggcaa gggcgatgcg
     9061 tttgcgaacc ctttatcgga cactgactgg ctgcgtcttg cgatggtgaa ggatctgcaa
     9121 cctggcgaaa tgaccgctga tgcagaagat gacacttatc tcgatgatga agatgcagac
     9181 tggaaaacga caactcaggg gcagaaatcc gtcggtgata cttcggcgac gctggcctgg
     9241 cgtccgggtg acagcggaca gaaaaaactg gttcagttgt tcgactccgg tgaaatctgc
     9301 gcgtttcgta tcaaatatcc caacggcact gttgatgttt tccgtggctg gctgagttca
     9361 ctgggtaaaa ccattgcctc aaaagacgtg atgacccgca ccgtgaaaat cagcggtgtg
     9421 gggcgtccgt atctggcaga ggagggcact gaaaccgtgg gcgttaccgg gctgacggtg
     9481 gcaccggcat ccgccagtat caatgtggga gcaaccacca cgctgacctt tacagtaaaa
     9541 cctgacggag ccagtgacaa agcgatcagt gtgcattcga cagatccaca gacagccact
     9601 gtgaccctga acggacttgt ggccacggtg aaaggcgtga agcagggcag tgtcagcatt
     9661 gtgggcatga cttctgacgg cgattttgtg gcagtggcta cggtggctgt cagcgccgca
     9721 ggttaacagg acgatactca tcatttgccc cggttattcg gggctttttt gcaggtggag
     9781 aatatgatgt ttctgaaaca gggcacgttt aattatgaaa aacagtccgt ggtgctcagt
     9841 gagctgtccg ggctgcagag aattgaatat ctggcgtttg ttcagcagcg aacggcaaag
     9901 tttgatgccg gggagggaga actgtcggag gctgaacgac agattgcttt tctgcggatg
     9961 gggatggata tcaatgcctg gctggtttcc cgatcactgt ggaatgcgga tcagtctaag
    10021 gatgtagaga cgctttgcgc atccgttatt acaacatggt cgtatgatgc cctgggggcg
    10081 ggggcggaga tggttctgtc gctgagcggt atgggggcca ttgataatgc cggggatgat
    10141 gagcatgagg cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc
    10201 ttgcccggga gttccggcgg gcagactggc ggcggatgct gtcggaaatg tcggccactg
    10261 agcttggtga gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg
    10321 cgcagtttgc ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agtgatgctg
    10381 cggtggctga tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag
    10441 aactgatgca tcttggagaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac
    10501 ctggtcattg atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa
    10561 aaccttctga atggtgccgc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt
    10621 atggagcgtc agactcaggc cgcccggcag acaacgcagg cggcttcttc ggctgcaaca
    10681 gccgcctccg tccatgcgca gacggtggag aagagcgcac aggctcatga acgcatggcc
    10741 cgcgaggtgg agaaaacccg ccagcgcatg gaggcactga gccagaaaat gcgcgatgaa
    10801 caggcgcagg ccatggctct ggcggaggct caggataaag cggctgctgc gttttatcgt
    10861 cagattgaca gtgtgaaaca ggccagtgcg gggctgcagg aattacagcg tattcagcag
    10921 cagatccgac aggccagaaa cagtggcggg attggtcagc aggattatct ggcgctgatt
    10981 tctgaggtta cggcgaaaac ccgtgttctt acacaggctg aggaagaggc tacccgacag
    11041 aaagtggcgt ttatccgtca gcttaaagag caggcaaccc gccagaatct ttcttcttct
    11101 gagttgcttc gtgccaaggc tgcccagctg ggggtaagca gtgctgcaga agtgtatatc
    11161 cgcaaaatgg agcaggcagg aaaagccacg cattcgctgg gtctgaaaag tgcagcggcc
    11221 cgccaggaga taggcgttct gataggtgaa ctggctcgcg gcaatttagg tgcgctgagg
    11281 ggatccggga taacgctggc taaccgtgcc ggatggatag acacactgat gtcaccgaaa
    11341 ggcatgatgc tgggcggggt tattggcggt attgccgcgg ccgtctatgg tctgggtaaa
    11401 gcctggtatg acggtcagaa ggagggggaa gaatttaacc gccagttgtc gctgacgggg
    11461 cattatgccg gagtcactgc cgggcagctg tggacgctta gtcgtgctat ttccgggaat
    11521 ggtatcacgc aacatgctgc agccggtgcg ctggctcagg tggtggggag tggtgcattt
    11581 cgtggaaacg atatcggtat ggtggcgaga gctgccgcac agatggagcg atcggttggc
    11641 cagtcggtca gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc
    11701 gcgaaggctc tggacaatga gctgcatttt cttactgcca ctcagcttga gcagatacgt
    11761 gtccttgggg atcagggacg gtccagtgat gctgcacgga tagccatgtc tgcactggca
    11821 gaggaaaccg gtcggcgtac tgcggatatt gataataacc tcaatgcgct gggcagtacg
    11881 ctgaagtatc tgtctgattt atggagtcgt ttctgggatg cggccatgaa tattggtcgt
    11941 gaagactcgc tggatgaaca gatttccgct ttacaggaga aagtgtcgcg ggcgaaaaga
    12001 ctcccctgga cggcatcatc ttctcaggtt gaatacgatc agcagcgtct taacgagctt
    12061 caggagaaaa aacgccagaa ggatttgcag gatgcaaaag agcaggcaga gcggaattat
    12121 caggagcaac agaaacgccg taatgctgaa aatgctgcac tgaaccggat gaatgaaacg
    12181 gaagcagcac gacatcagcg tgaaattgcg cgtattaatg ccatgcagta cgccgatcag
    12241 gctgtcaggg atgcggcgat acaacgtgaa aatgaacgtt acgagaaagc cctggcatcc
    12301 ggtaagaaaa aaacacgcga aacccgtaat gatgaggcca cccggttatt gctgcagtac
    12361 agtcagcaac aggcacaggt ggaaggacag attgctgctg ccagacagtc atcaggcatt
    12421 gccacggaaa ggatgacaga agctcataaa cagcttctgg ctctgcagca gcgcatcagc
    12481 gacctggacg ggaaaaaact gacggcagat gaaaagagtg tgctggcccg taaagatgaa
    12541 ctgattcagg cactgacgct gctggatgta aaacagcagg agcttcagaa acagacggca
    12601 ctcaacgagc tgaagaaaaa aacaattcag ctgaccagtc aactggctga agaagagcgc
    12661 gctcagcgtc agcaacatga cctggatatt gccacggtgg gtatgggtga tcagcagcgg
    12721 cagcgatatc aggtacaact gagtcttcgc cagaaatacc agcaacagct ggagcagttg
    12781 aggcgggata gtgagcagaa aggaacatat aacacggatg actacagaaa ggccgagcag
    12841 gcgctgacgg agagcctgaa ccgacaactg aatgagaatc gccgttactg gcaacagctt
    12901 gaagttgtgc agggtaactg gaaaaacgga gtcctgcgtg catttcagga ttttaccgtg
    12961 gatgcagata atacggcagg aacagcagaa caggtgttct cgtcagcctt cagctacatg
    13021 ggaaatggcc tggcaacttt tgtcactacc ggcaaactca atttcaaatc cttcacctct
    13081 tctgtgctgt cagatatggc gaaaatcctg gcgcaggcaa ccatgatgaa atcgataaaa
    13141 gggattggca gtgtactggg atttgatctc agcagccttt ccctgaatgc caatgggggg
    13201 atttatcagt ctgctgattt gagtcgttac agtggcacgg tggttaaccg tccgacgttt
    13261 tttgcttttg caaaaggcgc gggtgtgatg ggggaagcgg gacctgaagc cattctgcct
    13321 ctgcgtcgtg gtgctgacgg aaagctgggg gttgtggcgg atattggtgg ttcaggtatg
    13381 gcgatgtttg ccccgcagta caacatcgag atcaataacg atggcacgaa cgggcagata
    13441 ggtccggctg ccctgaaggt ggtttatgac cttgggaaaa aagcggcagc ggactttatg
    13501 caacagcagg cccgtgatgg tggtcggtta agtggagcat atcggtaatg gagacgtttc
    13561 actggaaagt gcgcccggat atgaatgtgg tatcagagcc gaaagtggtg acagtgaagc
    13621 tgggcgatgg ttatgaacag cgtcgtgcgg cgggactgaa taaccagttg tcgacttaca
    13681 gcgtgacgat acgtgttcgt aaatgtgaac acccatcttt aaaagccttt ctggaacggc
    13741 acggtggcgt ccgcgcattt cagtggacgc caccttatga ctggaaaccg atcagggtgg
    13801 tttgtcgcaa atggtcggca agcgtggggg cgctgtgggt aaccataacg gcagattttg
    13861 aacaggtcgt ggcataggag gctctgatgc aggatattcc acaggaaaca catcatgaga
    13921 cgacacgcct cactcagtca gcccaggtgg tgctctggga aatcgatctg acagaggtcg
    13981 gtggtgaacg ttattttttc tgtaatgagc agaacgaaaa aggtgagccg gttacctggc
    14041 aggggcggca gtatcaggca taccccattc aggggacggg atttgaactg aatggcaagg
    14101 gcagtgctgc ccgtccgaca ctgacggttt ctaacctgca cggcatggtc accgggatgg
    14161 cggaagacct gcagagtctg gtcggcggaa cggtggtcag gcgtaaggtt tacgcccgtt
    14221 ttctggatgc ggtgaacttc gtcaacggaa acagcgacgc cgatccggag caggaggtga
    14281 tcagccgctg gcgcatcgag cagtgcagcg aactgagtgc ggtcagtacc tcctttgtac
    14341 tgtccacacc gactgaaacg gatggctctg tttttccggg gcgtatcatg ctggccaaca
    14401 cctgcacctg gacctatcgc ggcgatgagt gcggttatag cggtccggcg gtcgcggatg
    14461 aatatgacca gccgacgtcc gatatcacga aggataaatg cagcaaatgc ctgagcggct
    14521 gtaagtttcg caataacgtc ggcaactttg gcggcttcct ttccattaac aaactttcgc
    14581 agtaatccca tgacagagac agaatcagcg attctggcgc acgcccggcg atgtgcgcca
    14641 gcggagtcgt gcggcttcgt ggtgagagcg ccggaggggg aaagatattt tccctgcgtg
    14701 aatatctccg gtgagccgga ggcgtatttc cgtatgtcgc cggaagactg gctgcaggca
    14761 gaaatgcagg gtgagattgt ggcgctggtc cacagccacc ccggtggtct gccctggctg
    14821 agtgaggccg accggcggct gcaggtgcag agtgatttgc cgtggtggct ggtctgccgg
    14881 gggacgattc ataagttccg ctgtgtgccg catctcaccg ggcggcgctt tgagcacggg
    14941 gtgacggact gttacacgct gttccgggat gcttatcatc tggcggggat tgagatgccg
    15001 gattttcatc gcgaggatga ctggtggcgt cacggtcaga atctctatct ggataatctg
    15061 gaggccacag ggctgtatca ggtgccgttg tcagcggcgc agccgggcga tgtgctgctg
    15121 tgctgttttg gttcatcggt gccgaatcat gccgccattt actgtggtga cggcgagctg
    15181 ttgcaccata ttcctgaaca actgagcaaa cgagagaggt ataccgacaa atggcagcga
    15241 cgcacacact ccctctggcg tcaccgggca tggcacgcat ctgcctttac ggggatttac
    15301 aacgatttgg ccgccgcatc gaccttcgtg tgaaaacggg ggctgaagcc atccgggcgc
    15361 tggccacaca gcttccgtcg tttcgccaga aactgaatga gggctggtat caggtgcgca
    15421 tttccgggcg tgatgcaggc gaaaatgaat tatctgcccg tcttaatgaa ccgctggcaa
    15481 atggtgccgt gatccacgtc gttccgcgtc tggcgggtgc caaaagtggc ggtgtgtttc
    15541 aggcggtgct gggtgcggcg ctgattgcgg tggcatggtg gaaccctgtt ggctggctgg
    15601 gggccgcggc tgtatcgggt ctgtatgcag caggggccag tatgatcct
//
LOCUS       DAZKFU020000102        15617 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZKFU020000102 DAZKFU020000000
VERSION     DAZKFU020000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.103"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..366
                     /gene="yiaB"
                     /locus_tag="ACHOQQ_RS12875"
                     /old_locus_tag="pgaptmp_002575"
     CDS             25..366
                     /gene="yiaB"
                     /locus_tag="ACHOQQ_RS12875"
                     /old_locus_tag="pgaptmp_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGU4470807.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFFGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            412..849
                     /gene="yiaA"
                     /locus_tag="ACHOQQ_RS12880"
                     /old_locus_tag="pgaptmp_002576"
     CDS             412..849
                     /gene="yiaA"
                     /locus_tag="ACHOQQ_RS12880"
                     /old_locus_tag="pgaptmp_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGU4470808.1"
                     /translation="MDNKISTYSLAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(891..1886)
                     /gene="wecH"
                     /locus_tag="ACHOQQ_RS12885"
                     /old_locus_tag="pgaptmp_002577"
     CDS             complement(891..1886)
                     /gene="wecH"
                     /locus_tag="ACHOQQ_RS12885"
                     /old_locus_tag="pgaptmp_002577"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGU4470809.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            2061..2360
                     /gene="ysaB"
                     /locus_tag="ACHOQQ_RS12890"
                     /old_locus_tag="pgaptmp_002578"
     CDS             2061..2360
                     /gene="ysaB"
                     /locus_tag="ACHOQQ_RS12890"
                     /old_locus_tag="pgaptmp_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGU4470810.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2455..3366
                     /gene="glyQ"
                     /locus_tag="ACHOQQ_RS12895"
                     /old_locus_tag="pgaptmp_002579"
     CDS             2455..3366
                     /gene="glyQ"
                     /locus_tag="ACHOQQ_RS12895"
                     /old_locus_tag="pgaptmp_002579"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGU4470811.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3376..5445
                     /gene="glyS"
                     /locus_tag="ACHOQQ_RS12900"
                     /old_locus_tag="pgaptmp_002580"
     CDS             3376..5445
                     /gene="glyS"
                     /locus_tag="ACHOQQ_RS12900"
                     /old_locus_tag="pgaptmp_002580"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGU4470812.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            5767..5919
                     /gene="hokA"
                     /locus_tag="ACHOQQ_RS12905"
                     /old_locus_tag="pgaptmp_002581"
     CDS             5767..5919
                     /gene="hokA"
                     /locus_tag="ACHOQQ_RS12905"
                     /old_locus_tag="pgaptmp_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HGU4470813.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(5896..5967)
                     /locus_tag="ACHOQQ_RS12910"
                     /old_locus_tag="pgaptmp_002582"
     CDS             complement(5896..5967)
                     /locus_tag="ACHOQQ_RS12910"
                     /old_locus_tag="pgaptmp_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470814.1"
                     /translation="MRNAVRKAGITSRRRLLLFQIAG"
     gene            complement(6107..6319)
                     /gene="cspA"
                     /locus_tag="ACHOQQ_RS12915"
                     /old_locus_tag="pgaptmp_002583"
     CDS             complement(6107..6319)
                     /gene="cspA"
                     /locus_tag="ACHOQQ_RS12915"
                     /old_locus_tag="pgaptmp_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGU4470815.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(6600..6890)
                     /gene="yiaG"
                     /locus_tag="ACHOQQ_RS12920"
                     /old_locus_tag="pgaptmp_002584"
     CDS             complement(6600..6890)
                     /gene="yiaG"
                     /locus_tag="ACHOQQ_RS12920"
                     /old_locus_tag="pgaptmp_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGU4470816.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            7324..8034
                     /gene="yiaF"
                     /locus_tag="ACHOQQ_RS12925"
                     /old_locus_tag="pgaptmp_002585"
     CDS             7324..8034
                     /gene="yiaF"
                     /locus_tag="ACHOQQ_RS12925"
                     /old_locus_tag="pgaptmp_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGU4470817.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(8084..9058)
                     /gene="ghrB"
                     /locus_tag="ACHOQQ_RS12930"
                     /old_locus_tag="pgaptmp_002586"
     CDS             complement(8084..9058)
                     /gene="ghrB"
                     /locus_tag="ACHOQQ_RS12930"
                     /old_locus_tag="pgaptmp_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGU4470818.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVDQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALMLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALLGKVEKNCVNPHVAD"
     gene            complement(9162..9821)
                     /gene="yiaD"
                     /locus_tag="ACHOQQ_RS12935"
                     /old_locus_tag="pgaptmp_002587"
     CDS             complement(9162..9821)
                     /gene="yiaD"
                     /locus_tag="ACHOQQ_RS12935"
                     /old_locus_tag="pgaptmp_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGU4470819.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            9974..12307
                     /gene="bisC"
                     /locus_tag="ACHOQQ_RS12940"
                     /old_locus_tag="pgaptmp_002588"
     CDS             9974..12307
                     /gene="bisC"
                     /locus_tag="ACHOQQ_RS12940"
                     /old_locus_tag="pgaptmp_002588"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGU4470820.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATEMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLEMFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(12276..12716)
                     /gene="yiaC"
                     /locus_tag="ACHOQQ_RS12945"
                     /old_locus_tag="pgaptmp_002589"
     CDS             complement(12276..12716)
                     /gene="yiaC"
                     /locus_tag="ACHOQQ_RS12945"
                     /old_locus_tag="pgaptmp_002589"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4470821.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRECIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(12713..13276)
                     /gene="tag"
                     /locus_tag="ACHOQQ_RS12950"
                     /old_locus_tag="pgaptmp_002590"
     CDS             complement(12713..13276)
                     /gene="tag"
                     /locus_tag="ACHOQQ_RS12950"
                     /old_locus_tag="pgaptmp_002590"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGU4470822.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            13434..14132
                     /gene="yhjY"
                     /locus_tag="ACHOQQ_RS12955"
                     /old_locus_tag="pgaptmp_002591"
     CDS             13434..14132
                     /gene="yhjY"
                     /locus_tag="ACHOQQ_RS12955"
                     /old_locus_tag="pgaptmp_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU4470823.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGSWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            14361..15563
                     /gene="yhjX"
                     /locus_tag="ACHOQQ_RS12960"
                     /old_locus_tag="pgaptmp_002592"
     CDS             14361..15563
                     /gene="yhjX"
                     /locus_tag="ACHOQQ_RS12960"
                     /old_locus_tag="pgaptmp_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4470824.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLIISLALSTTIRQPQQ
                     KVLREAHI"
BASE COUNT         3818 a         3797 c         4021 g         3981 t
ORIGIN      
        1 tcgctggtag atatggagat cgttatgaaa acctcaaaaa ctgtggcaaa actattattt
       61 gttgtcgggg cgctggttta tctggttggg ctatggatct catgcccatt gttgagtgga
      121 aaaggctatt tttttggcgt gttaatgaca gcaacttttg gcaactatgc atatcttcgc
      181 gcagaaaaac tcgggcaact ggataatttt tttacccata tttgccagtt agttgcgtta
      241 atcactatcg gtctcttgtt tatcggtgtt ttaaatgcac ctatcaatgc ttatgaaatg
      301 gtgatctacc ccatcgcctt ttttgtctgc ttgtttggtc aaatgcgttt gtttcgctcg
      361 gtatgagcaa cataaagctc ttacacattc aggaatgaaa ggaatactgt gatggacaac
      421 aaaatatcaa cctattcact ggcctttagt attgtgtcat ggatagctct cgttggtggt
      481 atcgttacct atctgttagg gctatggaat gcagagatgc agttaaatga aaaaggatat
      541 tattttgccg tactggtatt aggactgttt tctgcggcgt cttatcaaaa gaccgttcgg
      601 gacaagtatg aaggcatacc gaccacttcc atttattata tgacctgcct gactgtcttt
      661 attatctctg ttgcattact gatggtaggt ctgtggaatg cgacattatt actcagcgaa
      721 aaaggttttt atggactggc tttcttctta agcttgtttg gtgcagtagc ggtgcagaaa
      781 aatattcgtg atgccggaat aaacccacca aaagaaacac aggttaccca ggaagaatac
      841 agcgaataac tcacgtaagc ccggtcagtc caatgtgacc gggcttttac ttaactcact
      901 aatctgtttc tgtcgattcg ttgtaccagc atagaaagta acaaactcgc tgccaacgtc
      961 gcgcaaaaaa tccaaataat atccagtatt ggccaatttt taagctcaat tccccgggtg
     1021 cgcagtgcat ggataatcaa ggcgtggaat ccgtatatac ccaacgaatg gcgggagatt
     1081 aagccaagtc cgcgaatggt acgcgtatcc agcgtgtttt taaccagagt caatagcgcg
     1141 attgcgcaga taaaaaccat cggcccacag taaagatacc aggtatcggc aaaatttccg
     1201 cgccactgca attcatataa tgtcccgcga gagataataa aaactcccgt cgcaaacagt
     1261 gcggcgctca cccacgacag tgctttatgc tgtgtgtcca tcatccctat agcgcggccc
     1321 aacatgccat acagaatgta gtaaaaagta tcgccattga tatataagtt aattggcagc
     1381 cattcaaaac cgtcgatttt ctgcggcacg gtgtttgggt tagcgatgat accaatcacc
     1441 accattagca ccagcaacat ttttccgccg acgttcttca cctgaatcag cggtgaaacc
     1501 agataaatca ccgcaatcgc gaagaaaaac cataagtggt aaaacactgg cttttgcagc
     1561 aggtttttca gcgctaactc cacattaatg gaggtaaaca gtgcaatgta gagcagcgcg
     1621 attgcgctat aaaaaaacag acataagccg atacgcaaga aatggcgcgg ctgggcgctg
     1681 cgttcgccaa aaaagagata gccggaaatc atgaaaaata gcggcacgct gacacgagag
     1741 gcagagttca gaacattggc gatatcccag gtgacggggc taacactatg agcattggtc
     1801 acataccagg tagtggtgtg aatcatcacc accattaaac acgctatccc tcgcaggtta
     1861 tcaatccagt aaattttggg ctgcatctgt gtctctgtat ctggttaaaa aaagtctgac
     1921 cgataaatca ttcagttggc gcactggaat aatctgagtt ttaacactac agcttataga
     1981 ggcttaagga aattcgtaag atatcagcca ctataccgat ataaataata agactcacct
     2041 gcaaaccaga cggtaattta atgatgatga acgctttctt tccggcaatg gcgcttatgg
     2101 tgctagtggg ttgttctaca ccgtcacccg tgcagaaagc acaacgggta aaggttgatc
     2161 ctctgcgttc gttgaatatg gaagcgttat gcaaggatca ggcggcaaaa cgttataaca
     2221 ccggcgagca aaaaatcgac gtcaccgcct tcgaacagtt ccagggaagc tatgaaatgc
     2281 gcggttatac cttccgtaaa gagcagtttg tctgttcttt tgacgcagat ggccattttt
     2341 tgcatctttc catgcgttaa gtcctgcttt ttcccgtttc atactgtata tcttccatcc
     2401 agcgggtata ctgatccctt cctttaaatc cacacgtatc cagcacgaaa taatatgcaa
     2461 aagtttgata ccaggacctt ccagggcttg atcctgacct tacaggatta ctgggctcgc
     2521 cagggctgca ccattgttca accattggac atggaagtcg gcgcgggaac ctctcaccca
     2581 atgacctgtc tgcgcgcgct ggggccagaa ccgatggcgg ctgcttatgt tcagccttct
     2641 cgtcgcccga ccgatggtcg ctacggcgaa aaccccaacc gtttacagca ctactatcag
     2701 ttccaggtgg tcattaagcc atcgccggac aatattcagg agctgtacct tggttctctg
     2761 aaagagctgg gcatggaccc gactatccac gatatccgtt tcgtggaaga taactgggaa
     2821 aacccgacgc tgggtgcctg gggactgggc tgggaagtgt ggctaaacgg catggaagtg
     2881 acgcagttca cttacttcca gcaggttggt ggtctggagt gtaaaccggt taccggcgag
     2941 atcacctacg gtctggaacg tctggccatg tacattcagg gcgtagacag cgtttacgac
     3001 ctggtctgga gcgacggccc gctgggtaaa accacctacg gcgacgtgtt ccatcagaac
     3061 gaagtggagc agtccactta caacttcgaa tacgcggacg tggacttcct gttcacctgc
     3121 tttgagcagt acgagaaaga agcgcagcag ctgctggcgc tggaaaatcc gctgccgctg
     3181 ccagcttacg agcgtattct gaaagccgcc cacagcttca acctgctgga tgcgcgtaaa
     3241 gccatctccg tcaccgagcg tcagcgctac attctgcgca ttcgcaccct gaccaaagca
     3301 gtggcagaag catactacgc ttcccgtgaa gccctcggct tcccgatgtg caacaaagat
     3361 aagtaagagg cggctatgtc tgagaaaact tttctggtgg aaatcggcac tgaagagctg
     3421 ccaccaaaag cactgcgcag cctggctgag tcctttgctg cgaactttac tgcggagctg
     3481 gataacgctg gcctcgcaca cggcaccgtt caatggtttg ctgctccgcg tcgtctggcg
     3541 ctgaaagtag ctaacctggc ggaagcgcaa ccggatcgtg aaatcgaaaa acgcggcccg
     3601 gcgattgccc aggcgttcga cgctgaaggc aaaccgagca aagcggcaga aggttgggcg
     3661 cgtggttgcg gtattaccgt tgaccaggct gagcgtctga ctaccgataa aggtgaatgg
     3721 ctgctgtatc gcgcccacgt gaagggcgaa agcactgaag cactgctgcc gaatatggtt
     3781 gcgacttcgc tggcgaaact gccgattccg aaactgatgc gctggggtgc aagcgacgtg
     3841 cacttcgtgc gtccggtgca caccgtgacc ctgctgctgg gcgacaaagt cattccggca
     3901 accattctgg gcattcagtc cgatcgcgtg attcgcggcc accgctttat gggcgagccg
     3961 gaattcacca tcgataacgc cgatcagtat ccggaaattc tgcgtgagcg cgggaaagtc
     4021 atcgccgatt acgaagaacg taaggcgaag attaaagccg atgccgaaga agcagcgcgt
     4081 aagattggcg gtaacgctga cttaagcgaa agcctgctgg aagaagtggc ttcgctggtg
     4141 gagtggccgg tcgttctaac cgcgaaattt gaagagaaat tcctcgcggt gccgtctgaa
     4201 gcactggttt acaccatgaa aggtgaccag aagtacttcc cggtgtatgc gaacgacggc
     4261 aaactgctgc cgaactttat cttcgttgcc aacatcgaat cgaaagatcc gcagcaaatt
     4321 atctctggta acgagaaagt cgttcgtcca cgtctggctg atgccgagtt cttcttcaac
     4381 accgaccgta aaaaacgtct ggaagataac ctgccgcgcc tgcaaaccgt gctgttccag
     4441 caacagctgg gtacgctgcg tgacaagact gaccgcatcc aggcgctggc tggctggatc
     4501 gccgaacaga ttggcgctga cgttaaccac gcaacccgtg cgggcctgct gtctaagtgt
     4561 gacctgatga ccaacatggt cttcgagttc accgacaccc agggcgttat ggggatgcac
     4621 tacgcgcgtc acgatggcga agcggaagac gtagccgtcg cgctgaatga gcagtatcag
     4681 ccgcgctttg ccggtgatga cctgccgtcc aacccggtag cttgtgcgct ggcgattgct
     4741 gataagatgg atactctggc gggtatcttc ggtatcggtc agcatccgaa aggcgacaaa
     4801 gacccgtttg cgctgcgtcg tgccgcactt ggcgtgctgc gaattatcgt tgagaagaac
     4861 ctcaaccttg atctgcaaac gctgaccgaa gaagcggtgc gtctgtatgg cgataagctg
     4921 actaatgcca acgtagttga tgatgttatc gactttatgc tcggtcgctt ccgcgcctgg
     4981 tatcaggacg aaggttatac cgttgacacc atccaggcgg tactggcgcg tcgtccgact
     5041 cgtccggctg atttcgatgc ccgcatgaaa gcggtatcgc acttccgtac cctggaagca
     5101 gctgcagcac tggcggcggc gaacaaacgt gtatctaaca tcctggcgaa atctgacgaa
     5161 gtgctgagcg atcgcgtgaa tgcctctact ctgaaagagc cggaagaaat taaactggcg
     5221 atgcaggttg tggtgctacg tgacaagctg gagccgtact ttgcggaagg tcgttaccag
     5281 gatgcgctgg tcgaactggc agagctgcgt gaaccggtcg atgccttctt cgataaagtg
     5341 atggtcatgg ttgatgacaa agaattgcgt atcaaccgtc tgaccatgct ggagaaactg
     5401 cgcgaactgt tcctgcgcgt tgcggatatt tcgctgttgc agtaataacg ccgttattaa
     5461 aaagcctgcc atctggcagg ctttttttat cgctaaataa tacagcaacc tttaataatc
     5521 ttctgctgaa taaagattat ctcatatatt aattttatga gattttttag gattatatca
     5581 aggagaagaa acaaacttat taagctagaa tagccacggg tgcttgagac tgtttgtctc
     5641 aggtattcac cgaaaggcag acagagaaaa gccccacctg actataaatc aaagtgaggc
     5701 ccctctatgc atagacacca taaggttagc ctcttactcg ttggaaatca acacaggggg
     5761 ctgggaatgc cgcagaaata tagattgctt tctttaatag tgatttgttt cacgctttta
     5821 tttttcacct ggatgataag agattcactg tgtgaattgc atattaaaca ggggagttat
     5881 gagctggcgg catttttagc ctgcaatttg aaagagtaag agtcttcggc gggaagttat
     5941 tcccgccttt cttacggcgt tgcgcattct cattgcaccc aaatttattt ttcacaaaaa
     6001 taatactaga ttttattacg cgatcgatta tttatttcct gaaaaacaaa taaaaaaatc
     6061 cccgccaaat ggcagggatc ttagattctg tgcttttaag cagagattac aggctggtta
     6121 cgttaccagc tgccgggcct ttagcgccgc tttcgatggt gaaggacact ttctgacctt
     6181 cgtccagaga tttgtaacca tcgttctgga tagcagagaa gtgtacgaac acgtctttag
     6241 agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat tttacgatac
     6301 cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct ttcggcgata
     6361 tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg gtatcaacga
     6421 taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt caaaccgttg
     6481 atgtgcatta agccacgcat tggcgggtga tgcaacaatt atttttcata tttatgatta
     6541 atcggttgga aaacggtcct gtcatcagga ccgtaaaccg caataaagtg gataaaagtc
     6601 tattccatca actgcttact taatgccggg ttggcttgaa tcaaacgcat caattttagt
     6661 tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc gactgatacg
     6721 cccaaaaccc gggcgaaatc atcgattttt aatcctgtcc cttttcgtaa ctgctcaatt
     6781 tcggtacagg acgttgttct gtgcgtcagg gtaattttct gcgtttcatc tttaaaaaca
     6841 atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat tgaaaactcc
     6901 tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag tcgggaaaag
     6961 cagacacagc ggggtgaagt cagggcatgc tctggctatg attcgattca gccagtcatt
     7021 tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct taccactcct
     7081 ggtttgtaaa gatttcgaaa atctaacctt ctgcgaaggc gtaaatctct ggctacaaaa
     7141 aaggcaacgg caatatcatt ttttaacaat gaaacaacaa attaaccttg tttcattttc
     7201 tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg tcctggacta
     7261 tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga aaggagtaag
     7321 aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc gttattaatg
     7381 gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc gtttatcgac
     7441 ttcctgcaga atacagtgat gcgtagcggt gaacgtctac caaccctgac tgccgatcag
     7501 aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta ttctcagcag
     7561 gtgaatcagg cgatggattc cggtctgcgc ccggttgtag acagcgttaa cgcaattcgc
     7621 gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg ttcgctgggt
     7681 gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg atgctgcgca ctctgcgcta
     7741 aaacagagtg atgacctgaa accggtcttt gatcaggcat tcaccaaagt ggtaacgacg
     7801 ccagctgatg cattgcaacc attgattccg gcggcacaaa ctttcacgca acaactggtg
     7861 atggttggag actacattgc ccaacaaggt actcaggtga gctttgtagc aaatggcatc
     7921 cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc attaccggca
     7981 cagcatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca ataaagagta
     8041 aaagcccgag tgattatcgg gctttactcc acgcagtcgc ggcttagtcc gcgacgtgcg
     8101 gattcacaca gttcttctca acctttccta gtaacgcatc aatcaaatta tccacggcac
     8161 aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt actgcgacga
     8221 cgttggccat tgagagcaac ggcgaatcta cggaaagtgg ctcttgttcg aagacatcca
     8281 gcccggcggc gtgaatttcg cctttctgca atgcagcaat cagtgcattt tcatcaacca
     8341 ccgggccacg tccggcatta atgaatatgg cggaggattt cattttggcg aattgttctg
     8401 cgccaaatag atgatacgtc tcatcagtta acggcaggat caggcaaacg aaatctgact
     8461 cttgtaacag tgtatccaaa tcgcagtagc gggcgttgaa gcgttcttct gcttctttat
     8521 ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcgca cgttgtgcca
     8581 gcgccatacc gatccgtccc atcccgacaa tgcccagtgt tttatggtga acgtcagtgc
     8641 cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt tctgctacct
     8701 ccacaacccg acgagcggta gacaacatca gcgccatcag cgtatcggcg acggtttctg
     8761 ttaatacggt tggcgtgtgc atgagcagaa tttttcgggc ggtaagcgca tcgacatcaa
     8821 aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt ttttccagca
     8881 atgcggcatc tacattctcg tttgaaccca gtaaaccttc agcttcggca aaaattgctg
     8941 cattttgatc gacggtttgt gggctgaggt ttgccacctg gtgaacggtg aaatgctctt
     9001 gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac ggcttcatgc
     9061 ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa ggaaaactga
     9121 agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg gcttaaggta
     9181 atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc gattgggtta
     9241 gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt gatcaacgcg
     9301 ctggcaacgg aatccgcacg ctgttgggag agacgcatgt tcagatcgtg accacccgtg
     9361 ctgtcggtat aaccaatcac gttaaccgcc gttttcggat actctttcag caccattgcc
     9421 acgccggtca gggtgttagc gcccgcaggt ttcagggtcg cgctgctgct gtcgaaggtc
     9481 acattgttcg gcatattgag gataatgtta tccccgctgc gggttacact aacaccagtg
     9541 ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc cacgccgccg
     9601 cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc tttcttcgaa
     9661 gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc gatagcagat
     9721 ttacctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac cgccagagcg
     9781 ccactcacta cggcggcaat aagataaaca cgtttcttca ttgttaatcc ttaataacct
     9841 ttttattctt tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca tgaagattat
     9901 tcctgggaat actcggaaat ttgtaagtaa tatttaactg ctcaatacat ctaacctttc
     9961 aggagtcttc ggtttggcca actcatcctc acgatattcc gttctgactg ccgcccattg
    10021 ggggcccatg ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg gcgcgttagc
    10081 cacagaaatg gaaaactcct tgcagagcgc ggttcgcgac caggttcaca gcaatacgcg
    10141 ggtacgattt ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc cgcaaggcat
    10201 tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc ttattcacca
    10261 acaacataaa cgcattcgtg aggcttatgg tccggcatcg atttttgctg gttcctacgg
    10321 ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct atatggcgct
    10381 ggcaggcggt tataccgggc atctggggga ttattcgacc ggcgcggcac aggcgatcat
    10441 gccgtatgtc gtgggtggta gtgaagttta tcaacagcag accagttggc cgctggtgct
    10501 ggaacatagc gatgtcgtgg tgctgtggag tgctaaccca ctcaatacgc tgaaaattgc
    10561 gtggaatgca tccgatgagc aggggctttc ttacttttct gcactgcgtg acagcgggaa
    10621 aaagctgatc tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat
    10681 ggagtgggtg gcaccgcaca tgggcactga tgttgcgctg atgctgggga tcgcccatac
    10741 gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca caggttatgc
    10801 cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gcgaaaaccg ccgaatgggc
    10861 agcagagatt tgtggtgttg gcgcagcgaa aatccgcgag ctggcggcta ttttccacca
    10921 aaataccacc atgctgatgg ctggctgggg aatgcagcgc caacagtttg gtgagcaaaa
    10981 acactggatg atcgtcacgc tggcagcaat gttggggcaa atcggcacac ccggcggcgg
    11041 ttttggtctt tcttaccatt ttgccaatgg tggtaacccc acgcggcgcg ctgcggtact
    11101 ctcttccatg cagggcagct tgccgggtgg ctgcgatgcg gtagataaaa tccctgtcgc
    11161 ccgcattgtt gaagcactgg aaaaccccgg tggcgcatat caacacaacg gtatggaccg
    11221 acatttcccg gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca
    11281 ggataccaac cgcctgatcc gtgcctggca aaaaccggag ctggtggtga tctccgaatg
    11341 tttctggacg gcggcggcga aacacgcgga tatcgttctg cctgcgacta cctcttttga
    11401 gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaagca
    11461 agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttaagtga
    11521 acgctgggag aagggcggct atgcgcggtt tacggaagga aaaagtgagc tgcaatggct
    11581 ggaaatgttt tataacgttg cccgacagcg cggggcaagc cagcaggttg aattgccgcc
    11641 atttgctgag ttctggcaag ccaatcagtt aattgagatg ccggaaaacc cggacagcga
    11701 gcggtttatt cgcttcgctg atttttgccg cgatccgcag gcgcatccat taaaaaccgc
    11761 cagcggtaag attgaaatct tctcgcagcg tattgccgat tacgcttatc cggattgccc
    11821 ggggcatcca atgtggctgg agccggacga atggcagggt aatgccgaac cggaacagtt
    11881 acaggtactt tctgctcatc cggcgcatcg tctgcacagc cagctgaatt acagttctct
    11941 gcgcgaattg tacgcggtag caaaccgtga gcctgtgacc attcatcctg acgatgctca
    12001 ggcgcgcggc atacaagatg gcgatactgt tcggctgtgg aactcacgcg ggcaaattct
    12061 tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgcattc acgaaggggc
    12121 atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct
    12181 gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata cggcgctggc
    12241 atggctagaa aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag
    12301 ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa
    12361 ccctgcgcct ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga
    12421 tggggatggc gctgttgcac atactgcatc agcgccttac caataccgcg cctgacggcc
    12481 ttcggtgcga caaacatcgc tgccagaaat cggccttcca taatgctgac aaaaccaaga
    12541 agcttactgt cttcttccca gacccagttt tgtgcgttgg caagataggc atcccgcacc
    12601 agcggaatgc actcgcgcca gtaattcgct tttataaagg gatggcccca ggttgtactt
    12661 tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttccct aatcatggtt
    12721 tatttcccgg atagcaacag cagccaacca catgatcatt caccagccca catgcctgca
    12781 taaaggagta acagattgtg gtaccgacaa acttaaaacc acgttttttc agtgccttag
    12841 atagggcgtc ggaggcgggc gtagatgtgg ggatttcgct caacgttgtg gcttgtgtca
    12901 cctgtggctg atgatttaca aacgaccaga caaagtcggc aaacggttcg ccgttctgtt
    12961 ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttcccg cgatggcgga
    13021 taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgcg gcaaccttca
    13081 ccggatcgaa ctgatgaaag caggcgcgat agttttcgcg ttttttgagg acggtgatcc
    13141 acgataaccc agcttgctgc ccttccaggc agatcatttc gaacagtttt ttactgtcag
    13201 tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggtcc tgactcaccc
    13261 agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt
    13321 ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct
    13381 tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca
    13441 taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagtgtggta atcagtgcct
    13501 tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg caacccgggc
    13561 attccacaga gcgttacacc tgggatagtg atcatcaacc tgattacaac gatattttgt
    13621 cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa
    13681 cccctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg
    13741 ttacaactgg cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg
    13801 agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtacct
    13861 taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata
    13921 accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga
    13981 caaaccagaa cggcagctgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa
    14041 atattgccgc ctatgccgcg ctatctcagg cagaaaatac cactaacaat agcgattatc
    14101 tgtatacgat gggggttagt gccagatttt aacgtaacaa tcacaattga aaccattgaa
    14161 taacaatagt tgtggcgata gtgggtgcta acttaccaaa taataaattt ggtgaataat
    14221 tgtcgcgtca ttcattcctg aactaaggca tttcattccg ttctgatggc atttcatgcc
    14281 gtttttcccc aggcataaag tgcacttcgt tatggttgtc ggcagagatt tttccttttt
    14341 attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc tggctgacac
    14401 tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacctgg agcctgttta
    14461 acggcgcgct ttccgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct
    14521 tgttaagtct ggggctggca atttcgtctt ctgttgcggg caaattgcag gaacgttttg
    14581 gtgttaaacg cgtcaccatc gcttccggca ttttgctggg attagggttc ttcctgacag
    14641 cgcattctga caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg
    14701 atggcgcggg ttacctgctg acgctctcta actgcgtgaa atggttcccg gagcgtaaag
    14761 gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta
    14821 tcgacacgca gctgctggaa acggtcggtc tggaaaaaac ctttgtgatt tggggagcga
    14881 ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag
    14941 tgaaaaccag caatggtgtg gtggagaaag attacacgct ggcagagtcg atgcgtaaac
    15001 cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga
    15061 ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tctgcagcca
    15121 atgcagtcac tgttatttcc atcgccaacc tttcaggtcg tctggtgctg ggcattctgt
    15181 ctgacaaaat cgcccgtatc cgtgttatca ccattggtca ggtgatttcg ctggtgggta
    15241 tggcggccct gctgtttgca ccattgaatg cagtgacgtt ctttgcagcg attgcctgtg
    15301 tggcatttaa ctttggcggc actattaccg tctttccgtc actggtcagt gagttcttcg
    15361 gcctcaataa cctggcgaaa aactacggtg tgatttacct cggtttcggt atcggtagca
    15421 tttgtggttc gattatcgcc tcactgtttg gcggcttcta tgtgacgttc tacgtcatct
    15481 ttgctctgct gattatttcc ctggcacttt ccaccaccat tcgccagccg cagcagaaag
    15541 tgttgcgtga ggcgcatatt taatgagatg gcgttggtta ttaacgaagg ggcgagagag
    15601 cccctttttt tcgttta
//
LOCUS       DAZKFU020000103        15597 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZKFU020000103 DAZKFU020000000
VERSION     DAZKFU020000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.104"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..118
                     /gene="yecU"
                     /locus_tag="ACHOQQ_RS12965"
                     /old_locus_tag="pgaptmp_002593"
     CDS             <1..118
                     /gene="yecU"
                     /locus_tag="ACHOQQ_RS12965"
                     /old_locus_tag="pgaptmp_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGU4470825.1"
                     /translation="LKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
     gene            105..329
                     /gene="yecF"
                     /locus_tag="ACHOQQ_RS12970"
                     /old_locus_tag="pgaptmp_002594"
     CDS             105..329
                     /gene="yecF"
                     /locus_tag="ACHOQQ_RS12970"
                     /old_locus_tag="pgaptmp_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGU4470826.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(397..1119)
                     /gene="sdiA"
                     /locus_tag="ACHOQQ_RS12975"
                     /old_locus_tag="pgaptmp_002595"
     CDS             complement(397..1119)
                     /gene="sdiA"
                     /locus_tag="ACHOQQ_RS12975"
                     /old_locus_tag="pgaptmp_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGU4470827.1"
                     /translation="MQDKDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQLLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1349..2101)
                     /gene="tcyN"
                     /locus_tag="ACHOQQ_RS12980"
                     /old_locus_tag="pgaptmp_002596"
     CDS             complement(1349..2101)
                     /gene="tcyN"
                     /locus_tag="ACHOQQ_RS12980"
                     /old_locus_tag="pgaptmp_002596"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGU4470828.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGESKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(2098..2766)
                     /gene="tcyL"
                     /locus_tag="ACHOQQ_RS12985"
                     /old_locus_tag="pgaptmp_002597"
     CDS             complement(2098..2766)
                     /gene="tcyL"
                     /locus_tag="ACHOQQ_RS12985"
                     /old_locus_tag="pgaptmp_002597"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGU4470829.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2781..3767)
                     /gene="dcyD"
                     /locus_tag="ACHOQQ_RS12990"
                     /old_locus_tag="pgaptmp_002598"
     CDS             complement(2781..3767)
                     /gene="dcyD"
                     /locus_tag="ACHOQQ_RS12990"
                     /old_locus_tag="pgaptmp_002598"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGU4470830.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNVLGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3872..4672)
                     /gene="tcyJ"
                     /locus_tag="ACHOQQ_RS12995"
                     /old_locus_tag="pgaptmp_002599"
     CDS             complement(3872..4672)
                     /gene="tcyJ"
                     /locus_tag="ACHOQQ_RS12995"
                     /old_locus_tag="pgaptmp_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4470831.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4760..5311)
                     /gene="fliZ"
                     /locus_tag="ACHOQQ_RS13000"
                     /old_locus_tag="pgaptmp_002600"
     CDS             complement(4760..5311)
                     /gene="fliZ"
                     /locus_tag="ACHOQQ_RS13000"
                     /old_locus_tag="pgaptmp_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGU4470832.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPSSDIY"
     gene            complement(5357..6076)
                     /gene="fliA"
                     /locus_tag="ACHOQQ_RS13005"
                     /old_locus_tag="pgaptmp_002601"
     CDS             complement(5357..6076)
                     /gene="fliA"
                     /locus_tag="ACHOQQ_RS13005"
                     /old_locus_tag="pgaptmp_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HGU4470833.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6396..8108)
                     /locus_tag="ACHOQQ_RS13010"
                     /old_locus_tag="pgaptmp_002602"
     CDS             complement(6396..8108)
                     /locus_tag="ACHOQQ_RS13010"
                     /old_locus_tag="pgaptmp_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HGU4470834.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSSTLKLTSFNVNGKGAVDNAKATEADLTAAGFSQGAVVSGNSTWTKSTVTTFNA
                     ATATDVLASVSGGSTISGYTGTNNGLGVAASTAYTYNATSKSYSFDATALTNGDGTGA
                     TTKVADVLKAYAANGDNTAQISIGGSAQDVKIASDGTLTDVNGDALYIGSDGNLTKNQ
                     AGGPDAATLDGIFNGANGNAAVDAKITFGSGMTVDFTQASKNVDIKGATVSAEDMDTA
                     LTGQAYTVANGAQSFDVAAGGAVTATTGGATVNIGADGELTTATNKTVTETYHEFANG
                     NILDDDGAALYKAADGSLTTEATGKSEATTDPLKALDDAIASVDKFRSSLGAVQNRLD
                     SAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLS
                     LLQG"
     gene            8374..9780
                     /gene="fliD"
                     /locus_tag="ACHOQQ_RS13015"
                     /old_locus_tag="pgaptmp_002603"
     CDS             8374..9780
                     /gene="fliD"
                     /locus_tag="ACHOQQ_RS13015"
                     /old_locus_tag="pgaptmp_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU4470835.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            9805..10215
                     /gene="fliS"
                     /locus_tag="ACHOQQ_RS13020"
                     /old_locus_tag="pgaptmp_002604"
     CDS             9805..10215
                     /gene="fliS"
                     /locus_tag="ACHOQQ_RS13020"
                     /old_locus_tag="pgaptmp_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU4470836.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            10215..10580
                     /gene="fliT"
                     /locus_tag="ACHOQQ_RS13025"
                     /old_locus_tag="pgaptmp_002605"
     CDS             10215..10580
                     /gene="fliT"
                     /locus_tag="ACHOQQ_RS13025"
                     /old_locus_tag="pgaptmp_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGU4470837.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10658..12145
                     /gene="amyA"
                     /locus_tag="ACHOQQ_RS13030"
                     /old_locus_tag="pgaptmp_002606"
     CDS             10658..12145
                     /gene="amyA"
                     /locus_tag="ACHOQQ_RS13030"
                     /old_locus_tag="pgaptmp_002606"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4470838.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(12179..12592)
                     /gene="yedD"
                     /locus_tag="ACHOQQ_RS13035"
                     /old_locus_tag="pgaptmp_002607"
     CDS             complement(12179..12592)
                     /gene="yedD"
                     /locus_tag="ACHOQQ_RS13035"
                     /old_locus_tag="pgaptmp_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGU4470839.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKAGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12779..13984
                     /gene="yedE"
                     /locus_tag="ACHOQQ_RS13040"
                     /old_locus_tag="pgaptmp_002608"
     CDS             12779..13984
                     /gene="yedE"
                     /locus_tag="ACHOQQ_RS13040"
                     /old_locus_tag="pgaptmp_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGU4470840.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            13981..14214
                     /gene="yedF"
                     /locus_tag="ACHOQQ_RS13045"
                     /old_locus_tag="pgaptmp_002609"
     CDS             13981..14214
                     /gene="yedF"
                     /locus_tag="ACHOQQ_RS13045"
                     /old_locus_tag="pgaptmp_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGU4470841.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            14323..14991
                     /gene="yedK"
                     /locus_tag="ACHOQQ_RS13050"
                     /old_locus_tag="pgaptmp_002610"
     CDS             14323..14991
                     /gene="yedK"
                     /locus_tag="ACHOQQ_RS13050"
                     /old_locus_tag="pgaptmp_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HGU4470842.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            15102..15581
                     /gene="yedL"
                     /locus_tag="ACHOQQ_RS13055"
                     /old_locus_tag="pgaptmp_002611"
     CDS             15102..15581
                     /gene="yedL"
                     /locus_tag="ACHOQQ_RS13055"
                     /old_locus_tag="pgaptmp_002611"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4470843.1"
                     /translation="MFTIKTDDLTHPAVQTLVAYHISDMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGMGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAQD
                     RCLHRLSLETGTQAGFTACHHIYLKHGFVDCEPFADYQLDPHSRFLSLTLCENNELL"
BASE COUNT         3835 a         4006 c         3866 g         3890 t
ORIGIN      
        1 gctcaaaaaa caaccactgc tatttttagc aaagcttatg gtatactccg ccgccttaac
       61 atttttcacc gcaaattttc attgcaacat gacgaggaaa ataaatgagt acgcctgatt
      121 tttctactgc cgagaataat caagaactgg caaatgaagt ctcctgcctg aaagcgatgc
      181 tgacgctgat gctgcaggcg atgggacaag ctgacgcggg ccgcgtgatg ttaaagatgg
      241 aaaaacagct tgcgctgatc gaagacgaaa cccaggctgc agtattttcc aaaacggtta
      301 agcaaattaa acaagcctac cgtcagtaat ataaaaccgg ctgatagcgt gcctttcagc
      361 cggtttttgc atctggcacg caggacagaa aagagatcaa attaagccag tagcggccgc
      421 gtaacaggca acctgggtct tatttggtgc attaattttt ttctgcatgt ttttctggtg
      481 gaaattgacc gtattctccg agattgacaa aatcatcgct atctctgctg atgtcttccc
      541 ttccgccgtc cacctcagaa tttctttttc gcgcttgctg aaattcatct ctggcgtcat
      601 cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca gtaactgcat
      661 ttttaattgc agttcatcac taagaatggg tatttcgcgc gcgctgcaac gggaaaagga
      721 caaaaagccc agcgcccggt ttggcagcat taaatactga gtgacaccgc ggcgtaaacc
      781 atgtgcgcgc gcggcttccc ataacggctg tgcttcgctg aataagtcat cattccacat
      841 taaatggccc tgactaaagt tttcagggtt cagcaccgga tcaattgcga gaaagttttt
      901 tgcctgataa taactaaccc acgcctcagg gtaattggtg taaaaagcca ctttaggtcg
      961 cgtgaatggt accgggtggc gaacacataa cgaatagtaa tcgtactcca gcagctgagc
     1021 ctgaagctct atttcatggt agacctcttc tgcggcctcc atcctctgaa aacgcaacag
     1081 catcgtgcga cgccagctga aaaaatcctt atcctgcata gtaaaccgca acgcccctga
     1141 gagtgagtat catttataat gataattcaa actatcacat aaataagatt tatatataat
     1201 ttatattatt cagacaatga attacttttg caagccatcg cattctctta tgttattaat
     1261 gagttatgct gatttgttaa acagttttat cacgcttgaa atggcgttcc gccccgactg
     1321 tcgaatcgtc ggggctgatt ttttcttatt attgcagcag aaacttctcg aggaactggc
     1381 gggtgcgagg ctgctcggga tcggcaaata acgcttttgc ggccccctgc tcgactatcc
     1441 gcccctggtc cataaagatc gcccggtccg caacatcccg ggcaaagctc atttcgtgcg
     1501 tcacaatcac catcgtgcgc ttttcctgcg ccagctgacg gatggtgttc aggacttcgc
     1561 ccaccagctc tggatccagc gctgacgttg gctcgtcaaa caaaatcacc tcaggacgca
     1621 ttgccagcgc acgcgcaatc gcaacacgct gctgttgacc gccagacaaa cgacgtggat
     1681 agctggtttc tttaccggcc agcccaactt tcgccagtag ctcgcgagcg cgcgccgtgg
     1741 cctcttcttt cgattcgcct ttgacgatca ccggcccttc aataatgttc tccagcaccg
     1801 tacgatgcgg aaacaaatta aagttctgga agacaaaccc gacgtgctga cgcaactggc
     1861 gaatcagcga tttttgctga cttaatgaac gtgcagtatc aatagtgata tcgccaacgg
     1921 tgatagttcc cgcttccggt tgttccagca gatttatgct gcgtagcaac gtggttttgc
     1981 cggaaccact cggaccgata attgccacca cttcgccagg ctttacctca aggtcgatac
     2041 cgtgcagcac cgtctgaccg tgaaattttt tcaccaggtt cttaacttca atggcactca
     2101 ttttggttct ctctcctggc gattaagttg attctcaaaa tggttctgca acgtcgataa
     2161 cacagtcgcc atgatccagt agatgagcga agccgccagg tacatggtga acacctccag
     2221 cgtgcgcgag gtaatcaact gcgcttgacg gaacagctcc ggcacctgga ttgtcgcggc
     2281 cagcgacgta tcttttacca ggctgataaa gctgttcgac agcggcggca acgccacccg
     2341 cgccgcctgt ggcaaaatcg cgcgacgcat agtctgccat ggcgtcatac caatactggc
     2401 agctgcttcc cactgacctt tatcaataga agaaatggcg gcgcgaagcg tttcggcagc
     2461 ataggcggca gtgttgagcg acagaccaat cattgctgac ggaatgggat ctaattcgat
     2521 accaaactgc ggcaggccgt aatagatcat aaacagttgt gcgatgagcg gcgtaccacg
     2581 aaaaatagag atataaaaac gcgccagcca gcgcacgggc cagattggcg acaggcgcat
     2641 cagcgcgaga ataaacccga gcagtaagcc aaaaaacatg ccgccgatac tgagttgcag
     2701 cgtataccca gcccctttca gcaggaacgg caaggaatca ataaccagtt gtatactttc
     2761 ttgcatgagc gtttccggag ctaaacgtgg ggatgatagg cgaacagcgc aggcgcgccg
     2821 ccggtatgaa taaacagaat cggcccttca tctttgaagc gtttctgact gataccgtca
     2881 atcagacccg ccatcgcttt tccggtatac acaggatcaa gcagaatgcc ttccagccgc
     2941 gccagcaatt tcactgcttc catgccttcg tcgttcggca cgccgtagcc aggtgcaaaa
     3001 tagtcatccc agagtaaaat ttctgctgat gcggtcagct ccagttcttt cgcaatcgcc
     3061 tgttgtaggt taaccacttt cggcaattga tcggcaacgg aacgcgacac ggtcacgcca
     3121 atcagttcgc tttcaggcat caggtgttcc agcccaacag ccagtccggc gtgagttccg
     3181 gcactgcccg atgcgactac caccgacgaa atattaaccg ccccttcaca ctgttgcgcg
     3241 atttccagcg cactctccac ataacctagc gcgccaagaa cattagaacc gccaaccgga
     3301 atgacatatg ggcgaaagcc ttgtgcttcg actcgcgtcg ccagctcttc cagttgggca
     3361 ttgggatcgg tcagtgcgtc gcacatttca atctgggtat tgaacagatc cagcaacaag
     3421 cgattgccgt tggttaaata gttttctgcg gttgtgccaa taggattttc cagcagcgcc
     3481 acgcagtgca gaccgagttt ggcagcgact gcggcagtct ggcgcacatg gttagactgg
     3541 atcgccccgg cagtaatcag cgtatcggca ccttcacgca gagcatctgc cgcgagaaat
     3601 tctagcttac gtaatttatt gccgcccatt gccatggggg tgacgtcatc ccgtttgatg
     3661 aaaatttccc gtccaagata atcagaaaag cgcggcagat attcgagcgg cgttggcgcg
     3721 ccgataaact ccagccgtgg aaaacgggtt aaattatgca gtggcataac agcctccgat
     3781 gtgtgttgtt gtgattttct tattatgcac gctgaaaacg cgtaaataaa aaaggcgcta
     3841 gtgaaagcgc ccttttttgt cattatgctg attatttggt cacatcagca ccaaaccatt
     3901 tttcggaaag agcttgcaga gtgccgtctt tttgcatttc cgcaattgca tcattcactg
     3961 ctttcagcag gtcttcattt cctttacgca gcgccacgcc agactcctga cgggagaatg
     4021 cttcaccggt tactgccagc gtatcgttgg ttttcttcac cagatccagc gccgccagac
     4081 gatcaacgag gatcgcatcg atacgcccta cgcgcagatc ctgatatttg gtcgggtcat
     4141 catcataggt acgcacatcg acgccctgaa cattctgccg cagccactct tcatagttgg
     4201 tgcccagacc gacacccact tttttgcctt tcagatcagc agccgtttta atggtgcctt
     4261 cgttaccttt tttcaccagc gcctgaatac cagaaatggt gtacggggtt gagaaatcgt
     4321 attttttctt gcgctcatca gaaatggtga cctgattaat caccacatca atacgtttag
     4381 agtccagcga cgccagcata ccgtcccatt tggtcggttt tagggacgcc tcaacgccaa
     4441 gatgttttgc cagctgttgg gcaaattcca cttcaaaacc ggttaatttg ccgtcatccc
     4501 cctgaaaact gaacggcgga taagttcctt ccagacctac cagcagcgtg ccgcgctctt
     4561 taactttatt aagcagacct tcatctgcaa aacttttaac gctcatgccc gcaaccagcg
     4621 ccacggccat cacacccatc aatgcctgac gtcccagatg tgctaatttc atattcaccc
     4681 cgaatgttgt tatgtctgtt tgcagtgtag agccattgtt tgtaaacaca aaaacaactc
     4741 cgctacatct tattcttatt taatatatat cagaagaagg caggctggag gatttctgca
     4801 caagtccggt acaggtttgg cgctgatagt gttgatactt ccgtaacgcg atgcggtaat
     4861 tgttggtgct ggtggtgggc agccacgggg cgaggatttc atcaaggaaa ccgtcctgca
     4921 gctgatcgag ggaaatattt tgctcgtgta gatgattccc caaacggcgc agacgaacga
     4981 catattcacg caccgtacca gggctcattt cagtttgctc aaaaagaaat tgcttaaagc
     5041 cgataatatc gaaaaaatca ctctgcgttt tgcaatgtaa atcaccgcaa aatcggcaca
     5101 atgccgccca tgatttttgt tccgtttgcc agccattttc atcaagcagc gtgtccaggc
     5161 gggaaatctc gattttattc actattttgc cgtcgcgaac taaggtaatg cgatcgagta
     5221 atttacggca atgcgcgcaa tgggtctggc tgtgtttaaa gtctttaaga tagcggctta
     5281 atggccgtct tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aagttaaagt
     5341 gcggcattta ctgacgttat aacttaccca gtttagtgcg taaccgttta atagcctggc
     5401 tgtgtaactg actgacccgc gattccccga cctccagcac tgcaccaatc tctttgagat
     5461 tcagctcttc ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga
     5521 tggcttccat cacccgctgg cgcagattat tgtccagcag ttgttgtagc gggttttctc
     5581 gctgatgatc atcagtaacc agttcgatgc tatcgccgtg ctcttcgcgc cactcatcgt
     5641 aggagaagag ctggctgtta ttggtgtcga gcaacatttg gcgataatcg gcaatatcga
     5701 tccctaaacg ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt
     5761 gccctattgc ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac
     5821 ggctgcgaag ttcatccagc atagcgccac ggatacgctg cactgcgtaa gttgtaaatg
     5881 ccgttccttg tagggcgtca tagcgttcga cggcattaag taacccaatg ccgcccgcct
     5941 gtagcagatc gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt
     6001 gacgcaccag cgggacataa cgctgccata gcgagtgttt atccattaca ccttcagcgg
     6061 tatagagtga attcacgata aacagccctg cgttatatga gttatcggca tgattatccg
     6121 tttctgcagg gtttttaatc ggacgattag tgggtgaaat gaggggttat ttgggggtta
     6181 ccggtaaatt gcggacagaa aaacccccgc cggtggcggg ggagcacgtt gctgacaaat
     6241 tgcgcttgat gttgccggat gcggcgtaaa cgccttatcc ggcctacaaa aatgtgcaaa
     6301 ttcaataaat tgcaattcac cgtgtaggcc tgataagcgc agcgcatcag gcaatttggc
     6361 gttgccgtca gtctcagtta atcaggttag gacgattaac cctgcagcag agacagaacc
     6421 tgctgcggta cctggttggc ttttgccagc acggagttac cggcctgctg gatgatctgc
     6481 gctttcgaca tattggacac ttcggtcgca tagtcggcgt cctgaatacg ggactgcgct
     6541 tcagacaggt tggtagtggt gttgttcagg ttggtgactg cggaatccag acggttctgc
     6601 accgcaccga gggaagaacg gaatttgtct acagatgcga tagcatcgtc cagcgctttc
     6661 agcggatccg tggtcgcttc ggatttacca gtagcttcag tggtcagaga accgtcagcc
     6721 gctttgtaca gagccgcgcc gtcatcatcc agaatattgc cgttagcaaa ttcgtgataa
     6781 gtttctgtga cagtcttgtt ggtcgcagtc gtcagttcac catcagcacc aatatttacg
     6841 gtagcgccac ctgtagtagc ggttactgcc ccaccagcgg caacgtcaaa agactgtgcg
     6901 ccgttagcta cggtataagc ctgcccagtt aacgcagtgt ccatatcttc ggcggatacc
     6961 gttgcgccct taatatccac atttttgcta gcctgggtga aatcaacggt catgccgctg
     7021 ccgaatgtaa tcttcgcatc aactgctgca ttaccattcg caccgttgaa aataccgtcc
     7081 aacgttgccg catctgggcc gccggcctgg tttttagtca ggttgccgtc agaaccaata
     7141 tataaagcat caccattgac gtcagtcagg gtgccatcgc tggcaatttt aacgtcctga
     7201 gcgcttccgc cgatggagat ctgagccgta ttatcaccgt ttgctgcata ggctttcagc
     7261 acatcagcaa ctttagtggt ggccccagta ccatcgccat tggtaagtgc ggttgcgtca
     7321 aatgaataag acttgctggt tgcgttgtag gtatatgcag tagaagccgc tacgcctaat
     7381 ccattgtttg taccggtata accgctaata gtgctgcctc cgctaacgct tgccagcacg
     7441 tcggtagctg ttgctgcatt aaaggtagta acagtagatt tagtccaggt gctgttgcca
     7501 ctgacgactg caccttggga gaagcccgca gcggtcagat ctgcttcagt ggctttagca
     7561 ttatcaacag cgcctttacc gttaacattg aagctggtca gcttcaatgt agaagagtcg
     7621 atctttttca gatcaatcgt gatggtttca ccatcatttg cgccgacctg aattttcatc
     7681 gagccatctt tagacagcac gttcacgccg ttgaactggg tctgaccgga tacgcggtca
     7741 atttcgtcca gacgagattt gatttcgtcc tggatggagt ccaggtcaga atcggagtta
     7801 gtaccggtag tggcctgaac cgtcagttca cggatacgct gtaagttgtt gttgatttcg
     7861 gacagcgcgc cttcggtggt ctgtgcaaca gaaataccgt cgttggcgtt acgtgcagcc
     7921 tgagtcaggc ctttaatatt agaggtgaaa cggttagcaa tcgcctgacc tgcggcgtca
     7981 tccttcgcgc tgttaatacg caagccagaa gacagacgct cgatagaact cgacagcgca
     8041 gactggttct tgttgatatt attttgagtg atcagcgaga ggctgttggt attaatgact
     8101 tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt acgtattggg tttccacccg
     8161 tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa aacaaccttt agaatttttt
     8221 tcacaaacag ccattttttg ttagtcgccg aaatactctt ttctctgccc cttattcccg
     8281 ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc aggccgataa ccccggtatt
     8341 cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa gtatttcatc gctgggagtc
     8401 gggtcaggtc tggatttaag ttcaatcctc gatagcctca ccgccgcgca aaaagcgacg
     8461 ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta aacttagcgc ctacggtacg
     8521 ctgaaaagcg cgctgacgac tttccagacc gccaatactg cattgtctaa agccgatctt
     8581 ttttccgcca ccagcactac cagcagcacc accgcgttca gtgccaccac cgcgggtaac
     8641 gccatcgccg ggaaatacac catcagcgtc acccatctgg cgcaggcgca aaccctgacc
     8701 acgcgcacca ccagagatga tacgaaaacg gcgatcgcca ccagcgacag taaactcacc
     8761 attcaacaag gcggcgacaa agatccgatt accattgata tcagcgcggc taactcgtcg
     8821 ttaagcggga tccgtgatgc catcaacaac gcaaaagcgg gcgtaagtgc gagcatcatt
     8881 aacgtgggta acggtgaata tcgtctgtca gtcacatcaa atgacaccgg ccttgacaat
     8941 gcgatgacac tctcggtcag cggtgatgat gcgctacaaa gttttatggg ctatgacgcc
     9001 agtgccagca gcaacggcat ggaagtctcg gtcgccgccc agaatgcgca gctgacggtc
     9061 aacaacgtcg ccattgagaa cagcagcaac accatcagcg acgcgctgga aaacatcacc
     9121 ctgaacctga acgatgtcac cacgggcaac caaacgctga ccatcactca ggacacctcc
     9181 aaagcgcaaa cggcgattaa agactgggtg aatgcctata actcgctaat agataccttc
     9241 agcagcctga ccaaatacac cgccgtagat gcgggagctg atagccagag ttctagcaat
     9301 ggcgcactgc tcggcgactc cacgctgcgg acgattcaga cgcagttgaa atcgatgctg
     9361 agtaataccg tcagttcttc caactataaa acgctggcgc agattggtat cacgaccgat
     9421 cccagcgatg gcaaactgga actggatgcc gacaaactca ccgctgcact gaaaaaagat
     9481 gccagcggcg taggtgcatt gattgttggc gatggtaaaa aaaccggcat cacgaccacc
     9541 atcggcagca acctgaccag ttggctttcg acaacgggca ttattaaagc cgctaccgat
     9601 ggcgttagta agaccctgaa taaattaact aaagactaca acgccgccag cgatcgcatt
     9661 gatgcgcagg tcgcgcgcta caaagaacaa tttacccaac tggacgtttt aatgacctcg
     9721 ttaaacagca caagcagcta cttaacgcag cagttcgaaa acaacagtaa ttccaagtaa
     9781 tcattattca tcgggagaca ggtcatgtac gcggcaaaag gcacccaggc ctatgcacaa
     9841 attggcgtcg aaagcgccgt aatgagcgcc agccagcagc agctggtcac catgctattt
     9901 gatggagtgc tgagcgcact ggttagagcg cgcctgttta tgcaggacaa caatcagcaa
     9961 ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg agaacggact gcgggtgagt
    10021 cttgatgaag agagcaaaga cgaattaacc caaaacttga ttgctcttta tagctatatg
    10081 gtcaggcgct tgctgcaagc caatttacgc aacgatgtct ccgcagtcga agaagtggaa
    10141 gcattaatgc gtaatattgc cgatgcctgg aaagagtctt tactctcccc ttctttgatt
    10201 caggacccag tctgatgaac catgcaccgc atttgtattt cgcctggcaa caactcgtcg
    10261 aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca atgggacgaa ctcatcgcca
    10321 gcgaaatggc gtatgtgaat gcggtgcagg agattgcaca tttgactgaa gaggttgacc
    10381 cgtccaccac gatgcaggag cagctccgcc cgatgctgcg cctgattctc gacaacgaaa
    10441 gcaaggtaaa gcagttatta cagattcgaa tggatgaact ggcgaaactg gtcggtcagt
    10501 catcggtgca aaaatcggtg ttaagtgcct atggcgatca gggcggcttt gtgctggctc
    10561 cgcaggataa cctcttttga atctgaatga gtcgatggct cgcgaataat ccgattacgg
    10621 ctacgcttct aatgttcccc ttgaatggag tcgaagaatg cgtaatccca cgctgttaca
    10681 atgttttcac tggtattacc cggaaggcgg taagctctgg cctgaactgg ccgagcgcgc
    10741 cgacggtttt aatgatattg gtatcaatat ggtctggttg ccgcccgcct ataaaggcgc
    10801 atcgggcggg tattcggtcg gctacgactc ctatgattta tttgatttag gcgagtttga
    10861 tcagaaaggc agcatcccta ctaaatatgg cgataaagca caactgctgg ccgccattga
    10921 cgccctgaaa cgtaatgaca ttgcggtgct gttggacgtg gtagtcaacc acaaaatggg
    10981 cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat gctgacgacc gtacgcaaat
    11041 tgatgaagaa atcattgagt gcgaaggctg gacgcgttac accttccccg cccgtgccgg
    11101 gcaatactca cagtttatct gggatttcaa atgttttagc ggtatcgacc atatcgaaaa
    11161 ccctgatgaa gatggcatat ttaaaattgt taacgactac accggcgaag gctggaacga
    11221 tcaggttgat gatgaactcg gtaatttcga ttatctgatg ggtgagaata tcgatttccg
    11281 caatcatgcc gtgacggaag agattaaata ctgggcgcgc tgggtgatgg aacaaacgca
    11341 atgcgacggt tttcgtcttg atgcggtcaa acatattccg gcgtggtttt ataaagagtg
    11401 gatcgaacac gtacaggaag ttgcgccaaa gccgctattt attgtggctg aatactggtc
    11461 gcatgaagtc gataaactgc aaacgtatat tgatcaggtg gaaggcaaaa ccatgctgtt
    11521 cgatgcaccg ctgcagatga aattccatga agcatcgcgc atggggcgcg actacgacat
    11581 gacgcagatt ttcacgggta cattagtgga agccgatcct ttccacgccg taaccctcgt
    11641 tgccaaccac gacacccaac cgttgcaagc cctcgaagca ccggtcgaac cgtggtttaa
    11701 accgctggcg tatgctctaa ttttgttgcg ggaaaatggc gttccttcgg tattctatcc
    11761 ggacctctac ggtgcgcatt acgaagatgt cggtggtgac gggcaaacct atccgataga
    11821 tatgccaata atcgaacagc ttgatgagtt aattctcgcc cgtcagcgtt tcgcccacgg
    11881 tgtacagaca ttatttttcg accatccgaa ctgcattgcc tttagccgca gtggcactga
    11941 cgaatatccc ggctgcgtgg tggtcatgtc gaacggggat gatggcgaaa aaaccattca
    12001 tctgggagag aattacggca ataaaacctg gcgtgatttt ctggggaacc ggcaagagag
    12061 cgtaataacc gacgaaaacg gcgaagcaac cttcttttgc aacggcggca gtgtcagcgt
    12121 gtgggttatc gaagaggtga tttaaattca tccccggcgg caagccgggg agatttcatt
    12181 acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc ggcacactct gccgtcgggc
    12241 gatccacacg ctccatcgtc ataccatcgt actcaatggt gttaccgtca cgctcaacct
    12301 cgtacagctc acgttttacg gtaacgttag tgagatcatc agacatcaat gtcaacttac
    12361 ccggcacggc aatcacccgc tgccactgac ggcaatccag agtatcgccc gctttagtga
    12421 caatcaggct gcctatcgct tccggactga ccagcgcacg ttgcggccct ttggtctgcc
    12481 agtagcccgc cagccagtcc ggcgcggggg ttttcacgac attgttatag ttttcgactt
    12541 cggcacagcc cgccagcaac atgagcgcgc ctgcaattgc gagttttttc atcattcgtc
    12601 ctgcatgaga agaaaagagg attgtggcat taaagccctg atgtcgccag cctttccagg
    12661 ttcagagaga aatttgatct aactattatt tttgtgaatt attaccccga aaataaattt
    12721 gcatggtgta tgattcgcga ctcttgtttc aaaccttctg agttcagagg ctacattcat
    12781 gtcatggcag caattcaaac acgcctggtt gattaaattc tgggcgccca tccccgcggt
    12841 catcgcggcg ggtattctct ccacttacta ttttggcatc actggcacct tttgggctgt
    12901 cacgggtgaa tttacccgtt ggggcggtca gctcctgcag ctgttcggcg tccatgctga
    12961 agagtggggt tattttaaaa ttatccatct ggaaggatcg ccattaaccc gcatcgacgg
    13021 gatgatgatc ctcggtatgt ttggcggctg ctttgccgca gcgctgtggg ccaacaatgt
    13081 caaactacgc atgccgcgta gccgtatccg cattatgcag gccatcattg gcggcattat
    13141 cgccggtttt ggcgcgcgtc tggcaatggg ctgtaacctg gcggcgttct ttaccggtat
    13201 tcctcagttc tcgctgcatg cctggttctt tgccatcgcc actgccattg gttcatggtt
    13261 tggcgcgcgc tttaccctgc tgcccatctt ccgtattccc gtgaaaatgc agaaagtttc
    13321 tgccgcctca ccgctgacgc aaaaaccgga tcaggcgcga cgtcgttttc gtctcgggat
    13381 gctggtcttt ttcggcatgc tgggctgggc gctgctcacg gcgatgaacc aacccaaact
    13441 ggggctggca atgctgtttg gcgtcggctt tggtttactg attgaacgtg cgcaaatctg
    13501 ctttacttcg gcgttccgcg atatgtggat caccggacgt acccatatgg cgaaagcaat
    13561 cattatcggt atggcggtga gtgccatcgg gatcttcagt tacgtacagt taggcgttga
    13621 acccaaaatc atgtgggcgg gaccaaacgc ggtaattggt ggtttactgt ttggttttgg
    13681 catcgtgctg gctggcggct gcgaaaccgg ctggatgtac cgcgcggtag aaggccaggt
    13741 gcactactgg tgggtcggtc tgggcaatgt gatcggctca acgattctgg cgtattactg
    13801 ggatgatttc gctccggcgc tggccaccga ctgggacaaa atcaacctgc tgaaaacctt
    13861 tggcccgatg ggtggcctgc tggtgacata tttgctgttg tttgctgcgc tgatgttgat
    13921 tatcggctgg gaaaaacgct tcttccgccg tgcggcaccg cagactgcta aggagatcgc
    13981 atgaaaaata tcgttcctga ttaccgtctt gatatggtgg gtgaaccctg cccttatccg
    14041 gcagtcgcaa ctcttgaggc gatgccgcag ttaaaaaaag gggaaatcct ggaagtggtg
    14101 agcgactgtc cgcagtcgat caataatatt ccactggatg cgcgtaatca cggctatacg
    14161 gtgctggata ttcagcaaga cgggccgacc attcgttatt taattcagaa gtaatccctc
    14221 attcccgctg tgacccacag cgggaatatc gttgcctctg taccacatag attttacccg
    14281 tcccctctcc tattcttttc agtgtgctct caggaggttt ctatgtgtgg tcgctttgcc
    14341 caatcccaaa cgcgtgaaga ttaccttgcg cttctcgctg aagatattga acgcgatatt
    14401 ccctatgatc ccgaacccat tggcagatac aacgtcgcgc cgggaaccaa agtccttctg
    14461 ctcagtgagc gtgatgaaca ccttcatctg gatccggttt tctggggata tgctcccgga
    14521 tggtgggata aaccgccgct gattaacgcc cgcgtagaaa ctgcggcaac cagtcgtatg
    14581 tttaaaccgc tctggcaaca tggtcgggca atctgttttg ccgatggctg gtttgagtgg
    14641 aaaaaagaag gcgacaaaaa gcagccttat tttatctatc gtgctgatgg acaacctgtt
    14701 tttatggcag cgataggcag cacaccgttc gagcgcggtg acgaagccga aggatttttg
    14761 attgtcactg cagcagcaga ccagggtctg gtagatattc atgaccgccg cccgctggtt
    14821 ctgtcgccag aagctgcgcg ggaatggatg cggcaagaga ttagcggtaa agaagcctca
    14881 gaaatcgcgg ccagtggctg tgttccagca aaccagttct cctggcaccc ggtatcgcgc
    14941 gcggtgggta atgttaaaaa ccagggtgcg gagttaattc aacctgtttg ataagcctgg
    15001 agattattga tcaacaatac tgcgtcataa gtaatctcta ttagacaaag atttcattac
    15061 ctgttggcat attgcaaaaa taacaccaat acggaatcgt catgttcacg attaaaacag
    15121 atgatctcac ccatccagca gtgcaaacat tagtggctta ccatatttcc gacatgctgc
    15181 agcagtctcc ccccgaaagc agtcatgctt tagatgtgca aaaattacgt aacccgacag
    15241 tgacattctg gtcagtatgg gaaggcgaac aactcgcagg aatgggtgcg ctgaagttgc
    15301 tggatgataa acatggcgag ctaaaatcaa tgcgtaccgc gccaaattat ttacgtcgtg
    15361 gtgtcgccag tctgatttta cgccacattt tgcaggtcgc ccaggacaga tgccttcatc
    15421 gcctgagctt agaaacgggt acacaggctg gatttacggc ctgccatcat atttatttga
    15481 agcatggttt cgttgattgc gaaccgtttg ctgattatca acttgatcca cacagtcgat
    15541 ttttatcatt gacgctatgc gaaaataatg agttgctatg agccagacgc agcacat
//
LOCUS       DAZKFU020000104        15457 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZKFU020000104 DAZKFU020000000
VERSION     DAZKFU020000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.105"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            223..1080
                     /gene="focA"
                     /locus_tag="ACHOQQ_RS13060"
                     /old_locus_tag="pgaptmp_002612"
     CDS             223..1080
                     /gene="focA"
                     /locus_tag="ACHOQQ_RS13060"
                     /old_locus_tag="pgaptmp_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGU4470844.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1135..3417
                     /gene="pflB"
                     /locus_tag="ACHOQQ_RS13065"
                     /old_locus_tag="pgaptmp_002613"
     CDS             1135..3417
                     /gene="pflB"
                     /locus_tag="ACHOQQ_RS13065"
                     /old_locus_tag="pgaptmp_002613"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4470845.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3609..4349
                     /gene="pflA"
                     /locus_tag="ACHOQQ_RS13070"
                     /old_locus_tag="pgaptmp_002614"
     CDS             3609..4349
                     /gene="pflA"
                     /locus_tag="ACHOQQ_RS13070"
                     /old_locus_tag="pgaptmp_002614"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGU4470846.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            4448..4558
                     /locus_tag="ACHOQQ_RS13075"
                     /old_locus_tag="pgaptmp_002615"
                     /pseudo
     CDS             4448..4558
                     /locus_tag="ACHOQQ_RS13075"
                     /old_locus_tag="pgaptmp_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(4559..5989)
                     /gene="ycaM"
                     /locus_tag="ACHOQQ_RS13080"
                     /old_locus_tag="pgaptmp_002616"
     CDS             complement(4559..5989)
                     /gene="ycaM"
                     /locus_tag="ACHOQQ_RS13080"
                     /old_locus_tag="pgaptmp_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4470847.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIHNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(6199..7347)
                     /gene="ycaD"
                     /locus_tag="ACHOQQ_RS13085"
                     /old_locus_tag="pgaptmp_002617"
     CDS             complement(6199..7347)
                     /gene="ycaD"
                     /locus_tag="ACHOQQ_RS13085"
                     /old_locus_tag="pgaptmp_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4470848.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            7662..8288
                     /gene="ycaC"
                     /locus_tag="ACHOQQ_RS13090"
                     /old_locus_tag="pgaptmp_002618"
     CDS             7662..8288
                     /gene="ycaC"
                     /locus_tag="ACHOQQ_RS13090"
                     /old_locus_tag="pgaptmp_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGU4470849.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(8324..9187)
                     /gene="dmsC"
                     /locus_tag="ACHOQQ_RS13095"
                     /old_locus_tag="pgaptmp_002619"
     CDS             complement(8324..9187)
                     /gene="dmsC"
                     /locus_tag="ACHOQQ_RS13095"
                     /old_locus_tag="pgaptmp_002619"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGU4470850.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(9189..9806)
                     /gene="dmsB"
                     /locus_tag="ACHOQQ_RS13100"
                     /old_locus_tag="pgaptmp_002620"
     CDS             complement(9189..9806)
                     /gene="dmsB"
                     /locus_tag="ACHOQQ_RS13100"
                     /old_locus_tag="pgaptmp_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGU4470851.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(9817..12261)
                     /gene="dmsA"
                     /locus_tag="ACHOQQ_RS13105"
                     /old_locus_tag="pgaptmp_002621"
     CDS             complement(9817..12261)
                     /gene="dmsA"
                     /locus_tag="ACHOQQ_RS13105"
                     /old_locus_tag="pgaptmp_002621"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGU4470852.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNVRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(12561..13553)
                     /locus_tag="ACHOQQ_RS13110"
                     /old_locus_tag="pgaptmp_002622"
     CDS             complement(12561..13553)
                     /locus_tag="ACHOQQ_RS13110"
                     /old_locus_tag="pgaptmp_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4470853.1"
                     /translation="MSIKQLKDGRYKVDVRPQGTEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIDAWWVLDGRNQPYGDTYRVRLEKINREMRDPRASEMT
                     RKFMLGYRSDKLQAGLMPSSVNRDLCALSAMFTSLIDAEVFHNENPVRGIRKLKVRNT
                     EMAFLSDDEIDRLLERLEGDARRVAVLCLSTGARWGEAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDTVLSLIKTRKTGLLYQADYIRFRDILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLADSVHKLSID"
     gene            complement(13623..13970)
                     /locus_tag="ACHOQQ_RS13115"
                     /old_locus_tag="pgaptmp_002623"
     CDS             complement(13623..13970)
                     /locus_tag="ACHOQQ_RS13115"
                     /old_locus_tag="pgaptmp_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4470854.1"
                     /translation="MNSTLSISIGEKILMIRKSERLTRKQFFELTGIPVNTQRYYETG
                     RQDGIGSEILLKITKNLRFKKYALWLTTDETFEAGGQIAPALSLDGSSQSEGDLASID
                     VTQKSSRSGRNAG"
     gene            14072..14596
                     /locus_tag="ACHOQQ_RS13120"
                     /old_locus_tag="pgaptmp_002624"
     CDS             14072..14596
                     /locus_tag="ACHOQQ_RS13120"
                     /old_locus_tag="pgaptmp_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4470855.1"
                     /translation="MRKSIADKELSDTQALQENDSITESVGAANDDEHQEEKKKRTYK
                     KGATLRLDGPVAGLCSLEKGAAYIGLTKAALRVALQRNQMPGHKTRSNPEDENSDGVW
                     WFNAREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     KSKLEQHQYSEGNQ"
     gene            14648..14842
                     /locus_tag="ACHOQQ_RS13125"
                     /old_locus_tag="pgaptmp_002625"
     CDS             14648..14842
                     /locus_tag="ACHOQQ_RS13125"
                     /old_locus_tag="pgaptmp_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470856.1"
                     /translation="MTDSTQTKDLYSDLRFIIIQQQSGRMDPTRASKAWKEALTDQGI
                     DVISEVMAEVIFRLTRDESL"
     gene            14868..15203
                     /locus_tag="ACHOQQ_RS13130"
                     /old_locus_tag="pgaptmp_002626"
     CDS             14868..15203
                     /locus_tag="ACHOQQ_RS13130"
                     /old_locus_tag="pgaptmp_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HGU4470857.1"
                     /translation="MKQQRNARINNNTERYVARQHFRKNSATVTTEIHATPDGHPVKQ
                     TGKHTWAIGDTGIVIHKSFRSPITGRFNFTLTRGDDYFGQDFTFFEALRTADRLINGL
                     RFQYPGSKH"
     gene            15222..>15457
                     /locus_tag="ACHOQQ_RS13135"
                     /old_locus_tag="pgaptmp_002627"
     CDS             15222..>15457
                     /locus_tag="ACHOQQ_RS13135"
                     /old_locus_tag="pgaptmp_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HGU4470858.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTKDESLRNSLRGAIMGLYN
                     CALGSDDREALEELKSIMTELFEGEIVEPYFTGVA"
BASE COUNT         4207 a         3972 c         3608 g         3670 t
ORIGIN      
        1 cttttaatta actgttttag cggaggatgc ggaaaaaatt caactcattt gttaattttt
       61 aaaatttatt tttatttgga taatcaaata tttactccgt atttgcataa aaaccatgcg
      121 agttacgggc ctataagcca ggcgagatat gatctatatc aatttctcat ctataatgct
      181 ttgttagtat ctcgtcgccg acttaataaa gagagagtta gtgtgaaagc tgacaaccct
      241 tttgatcttt tacttcctgc tgcaatggcc aaagtggccg aagaggcggg tgtctataaa
      301 gcaacgaaac atccgcttaa gactttctat ctggcgatta ccgccggtgt tttcatctca
      361 atcgcattcg tcttctatat cacagcaacc actggcacag gcacaatgcc cttcggcatg
      421 gcaaaactgg ttggcggcat ttgcttctct ctggggctga ttctttgtgt tgtctgcgga
      481 gccgatctct ttacttccac agtgttgatt gttgttgcta aggcgagtgg gcgcatcacc
      541 tggggtcagt tggctaaaaa ctggctaaat gtctattttg gcaacctggt cggcgcactg
      601 ctgtttgtac ttttaatgtg gctttccggc gagtatatga ccgcaaatgg tcaatgggga
      661 ctaaacgtcc tacaaaccgc cgaccacaaa gtgcaccata cttttattga ggccgtctgc
      721 cttggtatcc tggcaaacct gatggtatgt ctggcagtat ggatgagtta ttctggccgc
      781 agcctgatgg acaaagcgtt tattatggtg ctgccggtcg cgatgtttgt tgccagcggt
      841 tttgagcaca gtatcgcaaa catgtttatg atcccgatgg gtattgtaat ccgcgacttc
      901 gcatctccgg aattctggac cgctgtcggt tctgcaccgg aaaatttttc tcacctgacc
      961 gtgatgaact tcatcactga taacctgatt ccggttacga tcggtaatat tatcggcggt
     1021 ggtttgttgg ttgggttgac atactgggtc atttacctgc gtgaaaacga tcaccattaa
     1081 tggttgtcga agtacgcagt aaataaaaaa tccacttaag aaggtaggtg ttacatgtcc
     1141 gagcttaatg aaaagttagc cacagcctgg gaaggtttta ccaaaggtga ctggcagaat
     1201 gaagtaaacg tccgtgactt cattcagaaa aactacactc cgtacgaggg tgacgagtcc
     1261 ttcctggctg gcgctactga agcgaccacc accctgtggg acaaagtaat ggaaggcgtt
     1321 aaactggaaa accgcactca cgcgccagtt gactttgaca ccgctgttgc ttccaccatc
     1381 acctctcacg acgctggcta catcaacaaa gcgttggaaa aagttgttgg tctgcagact
     1441 gaagctccgc tgaaacgtgc tcttatcccg ttcggtggta tcaaaatgat cgaaggttcc
     1501 tgcaaagcgt acaaccgcga actggacccg atgatcaaaa aaatcttcac tgaataccgt
     1561 aaaactcaca accagggcgt gttcgacgtt tacactccgg acatcctgcg ttgccgtaaa
     1621 tccggtgttc tgaccggtct gccagatgct tatggccgtg gccgtatcat cggtgactac
     1681 cgtcgcgttg cgctgtacgg tatcgactac ctgatgaaag acaaatacgc tcagttcacc
     1741 tctctgcagg ctgatctgga aaacggcgta aacctggaac agactatccg tctgcgcgaa
     1801 gaaatcgctg aacagcaccg cgctctgggt cagatgaaag aaatggctgc gaaatacggc
     1861 tacgacatct ctggtccggc taccaacgct caggaagcta tccagtggac ttacttcggc
     1921 tacctggctg ctgttaagtc tcagaacggt gctgcaatgt ccttcggtcg tacctccacc
     1981 ttcctggatg tgtacatcga acgtgacctg aaagctggca agatcaccga acaagaagcg
     2041 caggaaatgg ttgaccacct ggtcatgaaa ctgcgtatgg ttcgcttcct gcgtactccg
     2101 gagtacgatg aactgttctc tggcgacccg atctgggcaa ccgaatctat cggtggtatg
     2161 ggcctcgacg gtcgtaccct ggttaccaaa aacagcttcc gtttcctgaa caccctgtac
     2221 accatgggtc cgtctccgga accgaacatg accattctgt ggtctgaaaa actgccgctg
     2281 aacttcaaga aattcgccgc taaagtgtcc atcgacacct cttctctgca gtatgagaac
     2341 gatgacctga tgcgtccgga cttcaacaac gatgactacg ctatcgcttg ctgcgtaagc
     2401 ccgatgatcg ttggtaaaca aatgcagttc ttcggtgcgc gtgcaaacct ggcgaaaacc
     2461 atgctgtacg caatcaacgg cggcgttgac gaaaaactga aaatgcaggt tggtccgaag
     2521 tctgaaccga tcaaaggcga tctcctgaac tacgatgaag tgatggagcg catggatcac
     2581 ttcatggact ggctggctaa acagtacatc actgcactga acatcattca ctacatgcac
     2641 gacaagtaca gctacgaagc ctctctgatg gcgctgcacg accgtgacgt tatccgcacc
     2701 atggcgtgtg gtatcgctgg tctgtccgtt gctgctgact ccctgtctgc aatcaaatat
     2761 gcgaaagtta aaccgattcg tgacgaagac ggtctggcta tcgacttcga aatcgaaggc
     2821 gaatacccgc agtttggtaa caacgatccg cgtgtagatg acctggctgt tgacctggta
     2881 gaacgtttca tgaagaaaat tcagaaactg cacacctacc gtgacgctat cccgactcag
     2941 tctgttctga ccatcacgtc taacgttgtg tatggtaaga aaacgggtaa caccccagac
     3001 ggtcgtcgtg ctggcgcgcc gttcggaccg ggtgctaacc cgatgcacgg tcgtgaccag
     3061 aaaggtgctg tagcgtctct gacttccgtt gctaaactgc cgtttgctta cgctaaagat
     3121 ggtatctcct acaccttctc tatcgttccg aacgcactgg gtaaagacga cgaagttcgt
     3181 aagaccaacc tggctggtct gatggatggt tacttccacc acgaagcatc catcgaaggt
     3241 ggtcagcacc tgaacgttaa cgtgatgaac cgtgaaatgc tgctcgacgc gatggaaaat
     3301 ccggaaaaat atccgcagct gaccatccgt gtatctggct acgcagtacg tttcaactcg
     3361 ctgactaaag aacagcagca ggacgttatt actcgtacct tcactcaatc tatgtaatta
     3421 gatttgactg aaatcgtaca gtaaaaagcg tacaataaag gctccacgaa agtggggcct
     3481 tttttagcac gagagccttt tttgtcagct atctatactt taaggtgact gccaaaacag
     3541 actcgacgta gccttcgagc tgcgcaccaa cacggcctca gatgggccac atctggagaa
     3601 acaccgcaat gtcagttatt ggtcgcattc actcctttga atcctgtgga accgtagacg
     3661 gcccaggtat tcgctttatc acctttttcc agggctgcct gatgcgctgc ctgtattgtc
     3721 ataaccgcga cacctgggac acgcatggcg gtaaagaagt taccgttgaa gatttgatga
     3781 aggaagtggt gacctatcgc cactttatga acgcttccgg cggcggcgtt accgcatccg
     3841 gcggtgaagc aatcctgcaa gctgagtttg ttcgtgactg gttccgcgcc tgcaaaaaag
     3901 aaggcattca tacctgtctg gacaccaacg gttttgttcg tcgttacgat ccggtgattg
     3961 atgaactgct ggaagtaacc gacctggtaa tgctcgatct caaacagatg aacgatgaga
     4021 tccaccaaaa tctggttgga gtttccaatc accgcacgct ggagttcgcc aaatatctgg
     4081 cgaataaaaa tgtgaaggtg tggatccgct acgttgttgt cccaggctgg tctgacgatg
     4141 acgattcagc gcatcgcctc ggtgaattta cccgtgatat gggtaacgtt gagaaaatcg
     4201 aacttctccc ctaccacgaa ctgggcaaac acaaatgggt ggcgatgggt gaagaataca
     4261 aactcgacgg tgttaaacca ccgaagaaag aaaccatgga acgcgtgaaa ggcattcttg
     4321 agcagtacgg tcataaggta atgttctaat ttccccgtaa agcggcaact ttattgagtt
     4381 gccgcttttt tattccgcgc tgcataaata attcagagct ctatttctaa cgcccttaca
     4441 gcacagcttc caaatgtatt acccaaaccg ccagtatatg tcctgcggct tgcgcgcatt
     4501 tctcgattat gtaaaaacgg gtcagatctg ccagagtcag cgtcaccgac cacaataatt
     4561 atttactatt cgctttacgc gccagcagcg ggaatatcag tcctaatcct accagcacaa
     4621 acggcgttgc aacattcagc gccaactgga aggtccactc tgcagtgaag gcttccattt
     4681 tcgggaagat ccccgtcaaa caggcaaagg cggtaaaggc aaaacaccaa atcccgacgg
     4741 tcattgccag aggcttgtta tgaataaaga catattctgg tttatatttc tgcgccaggc
     4801 gaacgactgc aataaatgca acaaataccc ataaataacg cagcggcatc actaccgagt
     4861 taagattcaa cagccattta tagagattgt tcatatcacc gatgccgaga gtcggcagca
     4921 taatcaggat cgccaccagc accagggtca gaaaatagcc attaacgggc gtaccagaag
     4981 cgttggtacg acataaactg gctggaatat atttgctgtc agcatcacct aatagcactt
     5041 taagcggggc atcaatcgag aataccagcg cagctacttg tcccagggta ttcgcaatgg
     5101 cgtaaatcac cattaaagta ttacccatgt tgtaatactc gcccagcttc tgaaaggcgt
     5161 aatattgacc gttagtcatt aagtcatccg ggatattacg cgagtcaaac atcatcccca
     5221 tcgccagtga gcccagaatg gcacaaaccg caaccatcac cgccaggcat aacatccctt
     5281 ttggaaattc ttttcctggg ttgcgcgttt gattaacgta aggagaaatc ttctctgctc
     5341 cgccaaccgc gaaaaccagc attgaaatag tggtaatgta ggtaaagtcg atatgaggaa
     5401 tgaacgtttc ccaggtaatg tttgtggtcg caatatgcac ttcagtaatc gcaggcgcgg
     5461 ttaccgccat cgccacatac aggagcgaca taacaaacat cgcaatccct gccacagaac
     5521 cgacgatttt cagcgatttc ataccgcgtg aagcaaccca cataaagaag ataaacagca
     5581 ccagcgttaa cccctgtaac gctacgactg aatattcttt gattagcgaa ccgtcgcctt
     5641 tcatcgccca accgagcgca atcagtattg cctggggttt ttgtgccaga tagggaatat
     5701 gcaccaccca gtaggtccac gcggcgaggt aagccagtcc gggtcccatc gtgtgtttaa
     5761 tccaggtact gaccccgcct ttcccatctt tgaaggtcga gcctaactga ccaacaatta
     5821 gcgcataagg tgtgaaatag agcgcaaaga taaacaccca tgaaaaaaca accaccagcc
     5881 cctggttggc atagttatta acaacgttgc caaaacccca gacagtgata aaagacatca
     5941 gcgcaatgtt gtaccatcgc agctgttttt cctgaacatt cccagccata gacaaatcct
     6001 tcgccaaaat taaaggggca tctgtaaaac gagggattat gcgcggcaaa taagaagtgc
     6061 ggaaatagcg aaatgcaaaa ttataacctg ctgcacattt tatttttaaa cgaataaaat
     6121 ttggaaaaaa gcaagtcaga tagaagaaac aaaaaagcag agtgtgaaac agtaagttca
     6181 cactctgctt gaattcattt acacgtgagc aacgggtttc ggcgtatgac cggcgttgcg
     6241 aagcagcatc agcaaataga taaacgatac gctggcgatc atgataaaca ataaattatc
     6301 ggagaaattc tgcattagca tagcggtaaa tgacgggcca agcagacttc ccacggtata
     6361 gctcaacagt aaggcctggt tcatcgccac cagttgatga tgttcaactt tctcgcaagc
     6421 ccaggccatc gccaccggat atagcgtaaa gccagcggca ccgaggatga ataacgctgg
     6481 ggccatcgcc gcctggctaa gcatcgcgat actgccgaga atgacgacaa aaacctgaac
     6541 acgcaacacc agcagtcgac caaacttatc cgccagacgt ccaatcggcc attgtccgag
     6601 gatacccgca ctgaccagta ccgccatcca gaaaccaatg ctggcattac tcaccccctt
     6661 gtgattgagg tacaacggca tcaggccata tagagatccc agaacgattc ctgagataat
     6721 gcagccattc acgccaagcc gcgcctgacg gagttttagc attgacgtaa ttgacgtcga
     6781 atcatggttt tcagcctgct gattcaggac acgcgtaaac aacagcggta agatccctgc
     6841 caacgtcaaa cctgtaaccc acggcaatac gctcatcagc tcggttgaga ctttgctgac
     6901 cagtaactgg cctaaaaacg ttcccacgta ataaaccatc atatacgcag caagcaaacg
     6961 cccacggtta cgtgacgtcc cactgcacat cagcgcgctc tcaacgacta cccaaatcat
     7021 ggcacagccg acgcccgcga caaaacgcca cgccaaccag ctccagaaac caatcatcag
     7081 gccaaggccc gcacagccag cggcaaaaat gaaggaggcc agataatagc tgcggttaaa
     7141 gccaatgcgt ttaatgacat accctgtcag caatgtaccg acaaggttgc cggtaaaata
     7201 ggatgagctg acaacgccta cctgccatgt ggacatgtgt tcctgggcga gccaaagcgg
     7261 cacgagtgta tttaacaccg cgatcgccag agtcaacaaa agcaggccag acagcaaaag
     7321 catgacaggc cgggtatacg tggacatgaa ttaaaaccgt gaggaagttc aaatttcatg
     7381 cgcatcatgc caccgccaaa aacattgtca atgggtgaaa atacgcgcat atcgcaacgg
     7441 cggtattatc gatttatatt actcattcat aacgctaata ctgaagagat gattaatctc
     7501 atctgattgt ttttattgat ctaacacaaa tttcatacgc ttcgttcagt cgaaaaattt
     7561 catggtcaca gcaaactcat atttcccgtc tatgctttca gagcaatgca tcgcgtctga
     7621 ccgatttcag acgtaccact gactctcgaa aggagaagag gatgaccaaa ccgtatgttc
     7681 gtcttgataa aaatgatgct gccgttttgc ttgttgatca ccaggctggt ttactttccc
     7741 ttgtacggga tatcgaaccc gataagttta aaaataacgt gctggcgctg ggtgacctgg
     7801 ctaagtattt caacttacca actattctta ccactagttt tgaaaccggt cctaacggcc
     7861 cattagttcc agaactgaaa gcacaattcc ccgatgcacc ttacattgct cgcccgggca
     7921 atattaacgc ctgggataac gaagattttg taaaagcagt caaagcgaca ggtaaaaaac
     7981 agttaattat tgccggtgtg gtaaccgaag tttgcgtggc attcccggcg ctttcagcca
     8041 ttgaagaagg gtttgatgtc ttcgttgtga ctgacgcgtc agggaccttt aatgaaatta
     8101 cccggcattc ggcatgggat cgcatgtcgc aagcaggtgc gcaattgatg acatggttcg
     8161 gcgtggcttg cgagctgcac cgtgactggc gtaatgatat tgaaggactg gcgacgttgt
     8221 tctctaacca tattcctgat tatcgtaatt tgatgaccag ttatgacacg ttaacgaagc
     8281 agaaataaaa gagaaccggg gccgaaaccc cggttgttac tgattagctt gcgacggcca
     8341 tccccacggt catatgcaaa ccatagaata cgccgcgacc gattaattcc cctgccagaa
     8401 gcagaatgaa tgagacagat agtagcggaa ccgcaggctg ataacctttt agctgtggtg
     8461 caatccacaa gcacagggca acagccaaaa gcacgatccg ccaggacatc agcgcaccat
     8521 agtccggcac cagcgctgcg gcctgctgca cagaactatg aatagtcgcc agctctgcgc
     8581 cctgcatcac tgacatcacg ccactcacta ccagtgcaag tacagaaatg gctggcagca
     8641 gacgcatcgc ccagccatcg acgccagcca gactcagcaa caggtaaccc agtaacgggc
     8701 cgcccataaa cattgtcagg aagaagccca tcggcgtcca gatgctgtac caggtcggaa
     8761 cggtatcaat gctgttatag acacgcacca tcatccagac aaagactacg ccaagaacca
     8821 tcgtcactac cagccacagc gtacgcaatg ccggtgacag ctttttcagc attgccagca
     8881 accagccgat gccgcctacg gcaaaaaaga tggaaccgct ggcgatttcg ttactgagtg
     8941 ctgaagcccc tacccggttg agcgagttaa aggcgcgcat cggtgaacca agatggagca
     9001 tagaggcgat aaagccaatg cccatcagca cccataagcc aaacatgcag gcgataacac
     9061 gctgctgggc ttctgctcgc aggtcgcctt tgagcagcgc caaagccaga acgataaaac
     9121 cacccgctac acattgcccg aagaccgtga agatcatcag cggccattca tgccatccac
     9181 ttcccatctc acacctcctt cgggtttgcc agatagccag tggtatcccc ggtcgggcgg
     9241 ctattggcat tgggtttgat cacaatattc ggtttggtaa agtgagctcg cggcaacggc
     9301 gcaacggccg ccagatcgcc atgtttttta cgcagctcgt cgatagggcc gaaatccagc
     9361 gcccgcagcg gacaagattc aacacagatc ggctttttac cctccgcaac acggtcataa
     9421 caaccatcgc atttggtcat atggcctttc gtttcgttat attgcggtgc gccatacggg
     9481 caagccatat ggcagtagcg gcagccaatg cacacatctt catcgaccac aacaaaacca
     9541 tcttcacgtt tatgcatcgc accgctcggg cagactttag tacaagccgg atcttcgcag
     9601 tggttgcacg aaatcgacag atagtaggca aacacgttct ggtgccagac accgttatct
     9661 tcctgccagt cgccaccagc atattcataa atgcggcgga agctgacttc tggtgtcaaa
     9721 tctttgtagt ctttacaagc cagctcgcag gttttgcaac cggtgcaacg gctggaatca
     9781 ataaaaaatc catactgggt tgtcatcggt tactccttac accttttcaa cctgaacaag
     9841 gtttgtatgt gacggattcc ccttagcgag aggagacgga cgttgagtgg tcagtacgtt
     9901 aatacaacca cccttatcga cacgttttgc atccgggtca taccaggcac cttcacccag
     9961 tgcgaccaca cccggcatca ttcgtggcgt cactttcgcc tcaatatgaa cctcaccacg
    10021 aacgttaaag atcctgactt tatcgccgtt gtggataccg cgtttttggg catcaagcgg
    10081 gttgatccac atttcctgac ggcaagccgc tttcagcaca tcaacgttgc cgtaagttga
    10141 gtgaacgcga gatttatagt ggaaacctgt aagctgcagc ggatactgtt tgttcagcgg
    10201 atcctgataa ctttcaaagc ccggcgtgta gatcggcagt ggatcgatca catcgccttc
    10261 tggcaattcc caggtagcgg caatgtcagc cagcgcctgc gaataaatct caattttacc
    10321 cgatggcgtg gtcagtggat ttgcctgcgg atcttcacgg aaggctttat aagcaacgtg
    10381 atgcccttgt gggtcgcgct ttttaaagat cccctgcttg cggaactctt caaacgttgg
    10441 cagttcagga atcgcttccc gcgactgggc atacagatgc cgcatccact cttcctgggt
    10501 acggccttca gtaaactgtt gctcaacgcc aagacgtttt gccagttcgc tggtcatttc
    10561 atagatggtc ttacattcaa agcgcggttt aatcacctga tcgttgaaaa tcacgtaaga
    10621 catattcccg caggatgcat ccagtgcaaa gtccatctgt tcggaagcgg tacagtcagg
    10681 cagcaggatg tcagcatatt tcgccgatga agtcatgtgg cagtcgataa ccacaatcag
    10741 ctcgcacttc ttatcatcct gaaggatttc atgggtacgg ttgatttcag aatgctggtt
    10801 aatcaggcag ttaccggcat agttccagat cattttgatc ggcacatcca gcttatcttt
    10861 cccgcgcaca ccatcacgca gcgccgtcat ttccgggcca cgttcaatgg catcggtcca
    10921 cataaacatc gaaatgctgg tctggatcgg gttttccaag gtcggcatac ggacaaacgg
    10981 taagctgtat gaaccttcac gcgcgccgct gttccctcca ttaataccaa cgttaccggt
    11041 cagaatcgcc agcatcgaga tagcacgggt tgcgatttca ccgttagcgt gacgctgcgg
    11101 gccccatccc tggctgataa acgccggttt ggtactgccg atttcacgag ccagtttgat
    11161 gattttgtct gccggaacac cggtgatttg cgaggcccat tccggtgttt tagccacgcc
    11221 atctggccct tcacccagaa tataagcttt atagtggcca tttttcggcg cactggctgg
    11281 cagggttttc tcgtcatagc caacgcaata tttatcgagg aatgcctgat ccaccaggtt
    11341 ttcggtgatc atgacgtacg ccagaccgtt aaccagtgcg gcatctgttc ctggacgaat
    11401 agggatccac tcatcttcgc gcccggcacc ggtgtcggta tagcgcggat cgatgatgat
    11461 catgcgggca ttagatttct ggcgtgcctg ttcaagatag taagtcaccc caccgccact
    11521 cattcgcgtt tcgccagggt tattaccaaa cagcactacc agcttactgt tttcgatatc
    11581 cgacgggctg ttgccatctg cccagccgcc gtaggtatag ttcagacctt ccgcaatttg
    11641 cgcggaggag tagtcgccgt aatggttcag atagccgccg cagcagttca tcagccgcgc
    11701 gaccagggta tttcccggcg gccaggagcg ggtcatggtg ccacccagcg taccggtgcc
    11761 atagttaaga tagatggatt cgttgccgta ctctttgatc aggcgctgca tattggtcgc
    11821 gatgatgtcg taggcttctt cccagctaat gcgctcgaat ttgccttcac cgcgcgcccc
    11881 gactcgtttc atcggatatt tcaggcggtc cggattatag acacggcgac gcatggaacg
    11941 cccacgcaaa caggcgcgaa cctggtgcag gccgtcgtaa ttgtcatcgc cggtattgtc
    12001 cgtttcgaca tatttgattt caccgtccac gacgtgcata cgtagcgggc agcgactacc
    12061 acagttaact gtacaggcgc tccagataac cttttcgtct gattttgttg gaatggcgct
    12121 atcgacagcg tgcgcaatcc gactaaaagg taatgttaat gcgctgctgg ccattgccag
    12181 gccgccgatc gctgtcgttt ttaccaaacc acggcgactc acctcagcag ccaataccgc
    12241 atcagggatt ttcgttttca taatggctca ctcaagcttg ctcacgattg attccagtaa
    12301 caaaccgtat tgtgtaatta tttctatagc agatcgctat ataggcttgt atacatcgaa
    12361 taatttgaaa atcaaagaga taggtagatt ttcactcgaa agtagtatta cgtgtaagtg
    12421 aggagtaatt attgttcggt atcaaaggga gacaaaaaaa gcgcccgcag gagatttaac
    12481 acaccttact ccccttgatg atttacttat ttttcaatga ctaaatcaaa acaaggggag
    12541 caaattaacg caaattacaa ctagtcaatg gacagtttat ggacactatc agccagcgga
    12601 ttaaagctaa tcgcatcctg taaaaagtcc ggtgaaaaat gcgcatacgt catcgtttgt
    12661 tgaatcgtgg cgtgcccaag gattcgctgt aacgtaacga tatttccgcc gttcatcatg
    12721 aaatgcgtgg cgaacgtgtg acgcataacg tgagtcgcct gacctttggg taaatccggt
    12781 ttcacctcct gaaggatatc ccgaaaacgg atgtaatcag cctggtacaa tagcccggtt
    12841 tttcgggttt ttatcaacga aagcaccgta tcagaaacag gaaccgcacg ggattttcca
    12901 ttctttgtgt taaagaacat tacgcggtta ccaacgatat gctcaccacg taaaccagct
    12961 gcttcccccc accttgcgcc agtggaaaga cacagaacag caacgcgacg cgcatccccc
    13021 tcgagtcgtt caagcagccg atcaatctca tcatctgaaa gaaaggccat ttctgtattc
    13081 ctgaccttga gcttacgtat tccacgcacc gggttttcgt tatggaaaac ctccgcatca
    13141 atcagcgagg taaacattgc agacaaggca cacaaatcac gattaacact ggagggcatt
    13201 aatccggcct gtaatttatc tgaacgatac ccgagcataa actttcgcgt catctcactg
    13261 gcgcgggggt ccctcatctc acggtttatt ttttccagcc tgaccctgta agtatccccg
    13321 taaggctgat tccggccatc aagcacccac caggcatcta tcagttcgga taaccgacgc
    13381 tgatcagctg gtttggcctg ccacggttta tcgtggaaat tctgcaaaac atacctttca
    13441 aactcctgtg cctttgactt cagggtaaaa atcttacgga tccgctttcc ttccgtccct
    13501 tgcggtctta catcaacctt ataacgcccg tctttgagtt gcttaatcga catgattagc
    13561 ccctccaacg gatgaatcag ccgtacactc tctacgggat gaaacccgct cgatgtgtaa
    13621 cgtcagccag cattgcggcc tgatcgggat gatttttggg taacgtcgat tgaagccaga
    13681 tcgccctccg actgagaaga tccatcaaga gagagagccg gggcaatttg acccccagct
    13741 tcaaacgttt catcagttgt taaccaaagt gcatattttt taaaacgtaa attttttgtg
    13801 atctttaata agatctcact tccgattcca tcttgccttc ccgtttcgta atacctctgc
    13861 gtattaacag gaatccctgt aagctcaaag aattgcttcc ttgtaagcct ctcacttttc
    13921 cggatcatca agattttttc accgatactt attgacagtg tcgaattcat accactaaaa
    13981 tcctcgcaaa agtgtcgtaa tttcgacatc aaacaaatca aaacaaacct tcatgaggcg
    14041 cgacaagcgc accaacgcaa gaggatagca aatgcggaag tcaattgccg ataaagagtt
    14101 atctgacaca caagcccttc aggaaaacga cagcatcacc gaatccgttg gtgcggcaaa
    14161 cgacgatgaa caccaagagg aaaagaaaaa acgcacctac aaaaaagggg caaccttgcg
    14221 actggatggc ccggtagcag gactttgctc acttgaaaaa ggggcagcct atatcgggtt
    14281 gacaaaagct gcattgcgcg tggcgcttca gcgaaatcag atgcctggac acaagacccg
    14341 ttcaaaccct gaagatgaaa attcggatgg cgtatggtgg tttaacgcca gagagtggga
    14401 caagctggct gacgaactac cagaatgcga gccgccagaa tggcacaact ggaaaagcta
    14461 ctggacgtac gaccgacaga aaagaaagtt ttccccggca aacaaagaag actgccagac
    14521 catcaatgga aagcgggttt acacaggaag aaagtcaaaa ctggagcaac accaatacag
    14581 cgaaggcaac cagtgacatc aaaacaaacc gggatcatta agagcatcgg gatttgaagg
    14641 tgttgacatg actgactcca ctcaaacaaa agacttatac agcgatttac gattcatcat
    14701 catacaacag caatcaggca ggatggatcc aacacgtgca agcaaggcct ggaaagaagc
    14761 ccttaccgat caggggatcg atgtaattag cgaagtcatg gctgaagtga tttttaggct
    14821 aacaagagac gaatcactgt aatcatcgca taaggaccga gtgaagaatg aagcaacaac
    14881 gaaatgcacg catcaataac aacaccgaac gctacgttgc acgccagcac ttccgtaaaa
    14941 atagtgcgac cgtaaccact gaaatccacg caacaccaga cggtcacccg gtaaaacaaa
    15001 ccggaaagca cacctgggcg attggcgaca ctggcatcgt tattcacaaa agttttcgca
    15061 gtcctattac tggcagattt aatttcaccc tgactcgcgg agatgattac ttcggacagg
    15121 atttcacatt ttttgaagcc ttaagaacgg cagacagatt aatcaacggc ctgcgttttc
    15181 aatatccggg aagcaagcac tgataaataa aggattcagc catgatcgaa gcctacaaaa
    15241 tacttattaa taacgcgttg cagcgttacc acttcgaact tgcaaaccgg ggcgtaacta
    15301 aggatgaaag ccttcgtaac tcactgcgcg gcgcaattat gggactgtat aactgcgcgc
    15361 tgggcagcga tgaccgtgaa gcgctggaag aattaaagtc gattatgaca gaactgtttg
    15421 aaggtgaaat tgtcgagcct tacttcacag gagtggc
//
LOCUS       DAZKFU020000105        15371 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZKFU020000105 DAZKFU020000000
VERSION     DAZKFU020000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.106"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1567
                     /locus_tag="ACHOQQ_RS13140"
                     /old_locus_tag="pgaptmp_002628"
     CDS             <1..1567
                     /locus_tag="ACHOQQ_RS13140"
                     /old_locus_tag="pgaptmp_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HGU4470859.1"
                     /translation="AAKDSLDNLIKQQKNADESTLRAHKLEKKDVYSNAQRNAFTLGD
                     LPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSAGVQKIAAALQDYYINAGY
                     ITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWMLPFQRNDILNIRDIEQGL
                     ENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRASYNNWGDEETGRYQTTAV
                     GYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWEYGFFYSKSKSQQVIPLSY
                     ISLDYVGTSEYLSAKATRTVYRDKTKKFAGSAELIRRKSNYTLNGEELTLQARDMGNI
                     KLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGDVSPVSNIFNFEGNYTRQL
                     KNGYYNTAFFAQYAPRELTLQDQITVGDRWSIRGFENSVGLSGNDGFYIKNTLAFPLP
                     GMKANYYAGLDFGQVYQDASYGDESLVGAAVGIDGNIKSLEYNFSVSTPLKYPATLDI
                     DRVNVNFNFSYQM"
     gene            1583..1951
                     /gene="acpS"
                     /locus_tag="ACHOQQ_RS13145"
                     /old_locus_tag="pgaptmp_002629"
     CDS             1583..1951
                     /gene="acpS"
                     /locus_tag="ACHOQQ_RS13145"
                     /old_locus_tag="pgaptmp_002629"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU4470860.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTAHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIEQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            1965..2726
                     /locus_tag="ACHOQQ_RS13150"
                     /old_locus_tag="pgaptmp_002630"
     CDS             1965..2726
                     /locus_tag="ACHOQQ_RS13150"
                     /old_locus_tag="pgaptmp_002630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGU4470861.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHILQCDLACPQSVSALCEQIEREAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            2730..3278
                     /locus_tag="ACHOQQ_RS13155"
                     /old_locus_tag="pgaptmp_002631"
     CDS             2730..3278
                     /locus_tag="ACHOQQ_RS13155"
                     /old_locus_tag="pgaptmp_002631"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HGU4470862.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAHVGKKLISQGTIINFRETK"
     gene            3300..3581
                     /locus_tag="ACHOQQ_RS13160"
                     /old_locus_tag="pgaptmp_002632"
     CDS             3300..3581
                     /locus_tag="ACHOQQ_RS13160"
                     /old_locus_tag="pgaptmp_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4470863.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            3617..4777
                     /locus_tag="ACHOQQ_RS13165"
                     /old_locus_tag="pgaptmp_002633"
     CDS             3617..4777
                     /locus_tag="ACHOQQ_RS13165"
                     /old_locus_tag="pgaptmp_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HGU4470864.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCNID
                     GYYLLSEDIPAAELIASMNTILEKAEELDIQSLPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTDEDLLIFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQSVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKAVFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            4851..7409
                     /locus_tag="ACHOQQ_RS13170"
                     /old_locus_tag="pgaptmp_002634"
     CDS             4851..7409
                     /locus_tag="ACHOQQ_RS13170"
                     /old_locus_tag="pgaptmp_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HGU4470865.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVADSERFA
                     KWFENARAAEVLHDIEYSDLNDDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSQARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHLELGSVT
                     FPYDTLEEAKELLAVLDESNQFAELFDEEYSFSDATLPETASNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAAARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALKHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            7414..8637
                     /locus_tag="ACHOQQ_RS13175"
                     /old_locus_tag="pgaptmp_002635"
     CDS             7414..8637
                     /locus_tag="ACHOQQ_RS13175"
                     /old_locus_tag="pgaptmp_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HGU4470866.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHAAKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAITMFAGERQILVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMSNTQAGRAK"
     gene            8634..9587
                     /locus_tag="ACHOQQ_RS13180"
                     /old_locus_tag="pgaptmp_002636"
     CDS             8634..9587
                     /locus_tag="ACHOQQ_RS13180"
                     /old_locus_tag="pgaptmp_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4470867.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGPFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERRQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            9598..10305
                     /locus_tag="ACHOQQ_RS13185"
                     /old_locus_tag="pgaptmp_002637"
     CDS             9598..10305
                     /locus_tag="ACHOQQ_RS13185"
                     /old_locus_tag="pgaptmp_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4470868.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            10289..10945
                     /locus_tag="ACHOQQ_RS13190"
                     /old_locus_tag="pgaptmp_002638"
     CDS             10289..10945
                     /locus_tag="ACHOQQ_RS13190"
                     /old_locus_tag="pgaptmp_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470869.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVKQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVSEWDSEELTQVLLPLMKGE
                     "
     gene            10946..12256
                     /locus_tag="ACHOQQ_RS13195"
                     /old_locus_tag="pgaptmp_002639"
     CDS             10946..12256
                     /locus_tag="ACHOQQ_RS13195"
                     /old_locus_tag="pgaptmp_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4470870.1"
                     /translation="MSGEILAVNSVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKLRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSTKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            12265..13053
                     /locus_tag="ACHOQQ_RS13200"
                     /old_locus_tag="pgaptmp_002640"
     CDS             12265..13053
                     /locus_tag="ACHOQQ_RS13200"
                     /old_locus_tag="pgaptmp_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HGU4470871.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLVGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            13050..14438
                     /locus_tag="ACHOQQ_RS13205"
                     /old_locus_tag="pgaptmp_002641"
     CDS             13050..14438
                     /locus_tag="ACHOQQ_RS13205"
                     /old_locus_tag="pgaptmp_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470872.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMNLDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSYSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHHETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLIDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFSLFGD
                     TLGLYLGFKYYL"
     gene            14452..>15371
                     /locus_tag="ACHOQQ_RS13210"
                     /old_locus_tag="pgaptmp_002642"
     CDS             14452..>15371
                     /locus_tag="ACHOQQ_RS13210"
                     /old_locus_tag="pgaptmp_002642"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU4470873.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMLNDFAGVDQQRVMHCRKAFT"
BASE COUNT         3929 a         3506 c         4071 g         3865 t
ORIGIN      
        1 tgcagcgaaa gacagtcttg ataatttgat caagcagcaa aaaaatgcag atgaaagcac
       61 gttgcgcgcg cataaattag aaaaaaaaga tgtttactca aatgcacaac gtaatgcgtt
      121 tactctgggt gatctgccgc aggaaaagaa ttgctttatc atcaatcgga ttgaactgga
      181 agataacttt ctgggtggtc agttgatttc tgaagtgcaa aaagcgattg ccggacgttg
      241 tgtcggtagt gctggtgtgc aaaaaattgc tgctgccttg caggattatt atattaatgc
      301 cggatatatc acaacgcgaa ttagtatccc ttcgcaggat atttctaaag gcaagctacg
      361 tttccaggtt aatgccggga agatagaaaa aacgattgtc gaaggtaatg atatcagtga
      421 atggatgtta ccttttcagc gtaacgatat tctcaatatt cgtgatattg aacaggggct
      481 ggaaaatctt cagcgagtac ctgacgttga tgtgaaaatt aatattgaac cgggaacacg
      541 ggatggttac agcatcgttc atattgatac acaccggggg aaaaactgga gcgttcgggc
      601 aagttacaac aattggggcg atgaagaaac gggccgttac cagacaaccg cggtgggtta
      661 tttgtttaat ccagcgaaaa tgggggatct gttttatctc gccggtacgc gtagtactac
      721 cggacagtat gaaaacgtca gtagttatta ttccattccg gtggggtact gggagtacgg
      781 ctttttctat agcaaaagta aatcgcagca ggtgattccg ctttcgtaca tctcgctgga
      841 ttatgttggc accagcgaat acttgagcgc aaaagcgacg cgtaccgttt atcgcgataa
      901 aacaaaaaaa ttcgccggta gtgcagaact tattcgtcga aagtcgaatt acacgcttaa
      961 tggtgaggag ctgacattac aggcgcggga tatgggaaat atcaagctgg ggctaaatta
     1021 taagcaacag ttacccggcg cgttctggga ttcaaccctc tcgtggcaac gctttttaac
     1081 ctggttcggt ggtgaaaaaa cgccggatat gcaatatggc gacgttagcc cggtgagcaa
     1141 tatctttaat tttgagggta actatacccg acaactaaag aatgggtatt acaatactgc
     1201 tttttttgcc cagtacgcac cgcgtgaact gactttgcaa gatcaaatta cagttggcga
     1261 tcgttggagt attcgcgggt ttgaaaatag cgtaggtttg tcagggaatg atggttttta
     1321 tataaaaaat acgctggcat ttcctttgcc tggaatgaaa gctaactatt atgccggtct
     1381 tgattttggg caagtgtatc aggacgcttc ttatggcgat gaatcactgg tgggcgcggc
     1441 agttggtatt gatggaaata ttaaaagtct tgagtataac ttttccgttt ctaccccatt
     1501 aaagtatccg gcgacattag atattgatcg ggtgaatgta aattttaatt tttcgtacca
     1561 aatgtaaagg ataacgggac tcatgagaat agggacagat attgttgaaa ttgccagaat
     1621 aaccaaagcc attattaatg gagagatggc tttttatgag cgtgtttata ctgctcatga
     1681 gcgcttactc attaaccctg cagaacctga ttatgaacgg gcctctggat tctgggcggc
     1741 gaaagaagcg ttagttaaag cgattggttg tggttttcgt gatggcgtac gtttccagga
     1801 tattgaaata aagcatgatg cagccaaagc accttatttc gttatttccg gaagactaca
     1861 agaaattttt atcgagcagg ggttgcaaaa taactccctg agtatttcgc attgccgtac
     1921 ccacgccatt gcgactgtca tcgtttatta actcaacata tgtgatgaat atgtacaaat
     1981 ataatgattt aaatgaaaaa accgtactgg tgacaggagc cagtggtgat attggactgg
     2041 gtatttgcga aaaatatctg gagcagaact gtgatgttta tgcgctctat aaatccaacg
     2101 acgtgcaatt aacggcgctg aaagcaagcc atcctgcagg cgataaactg catattttgc
     2161 agtgcgatct cgcctgcccc cagagcgtga gcgcattgtg cgaacagata gagcgcgaag
     2221 ctggcaagat tgatgtgctg gtgaataacg cgggcatcgt taaagacagc ctgtttgcct
     2281 cgatgagcta tgaggatttc acccaggtta tcgaaaccaa catgttcagc attttccgtc
     2341 tgacgaaaga cgcgctgatg ttgcttcgtg cggcagaaaa tcccgccatt attaacgttg
     2401 cctctattgc agcgctgatc ccaagcgtcg gtcaggcgaa ttacagcgcg tcaaaagggg
     2461 cgatcctagg cttcacccgc acgctggcag cagaaatggc accctggggc gtgcgcgtca
     2521 acgcggtcgc accgggaatg attgaatcga aaatggtgaa aaaagtatcc cgcgcagtgg
     2581 tccgtgcggt aaccagtacc atcccgttac gccgtctggg gaaatgtgaa gaagtggcga
     2641 acaccatcgt cttcttaagt tcctctgcct ccagctacat tgtcggccag accatcgtta
     2701 tcgatggcgg cctggtcatg cggtgagtta tgtctaaata cacgattcct tcgaaaatct
     2761 tcctcgaaat gggcggctgg cgtcagccgc tgctgatggt cgataaaatt gcagattata
     2821 aatacggtga aaatggcttt gtcagtgtcg tgaaacacgt cacctacaac gaaccgtatc
     2881 tgctggggca tttcccggaa gatccgataa tgcccggggt aattatttcg gaaatcttcg
     2941 gccaggccag cgagtatctc tctttcctga ccgacatttg cgacatctgg cgcgaacgtt
     3001 ttgaagaaga gctgaagtcg ttacgtgata ttcacgcacg cattcaccgc cctgaaatgc
     3061 tggagattat ccgtacccgg cgcagccagg tacgcggcgt tctggcggct cagaacctga
     3121 aatttaaaga tatcgcctat ccgggtgatt ctattgatgt tgtcagcaaa ttggcttttt
     3181 cggatgccag tggttttaag cactattccg tgaccgcgca tgtcggtaaa aagctgatta
     3241 gtcagggcac cattattaat ttccgtgaaa ccaagtaatc caataagaga gtaaacacta
     3301 tgtctaccat gaatgacgct attgaacagc gtaaagaagt tctgatgacc attaaaactg
     3361 agatcatcca gcgactgaat attcaacgcg atgaagcgca aattgatgat gatactgcgc
     3421 tgttcggtaa tggttttaaa ctggactccg tcgatgccac ggaaattgtc gtcctgctgg
     3481 ataaagtttt tgatatcaag atcagcgaaa gtgatgaccc gtcatatatg cgcagtatca
     3541 atagtctggc caccttcgtt attcaaaaga aaaacgacta actacttttt aaagaattaa
     3601 atatcaggac acgtttatga aatctcttgc tgaatttcac ttgaaaaata atgcggtaat
     3661 gggtgtgtat aacaaccgta ccctaccttc ttcttatcac gatgctatga ctgaatataa
     3721 agctgtacgc gaaaacgcgc tgctggtgga ctattctcac ctctctatcg ttagcgtcat
     3781 gggcgatgat gcctgggcgc tgatcaacca gctggtttcc gcagatgtct ccatcattcg
     3841 tgatgagcag gcaatctact cactggtgtt gaacgaagag ggcaccattc gcggagacgt
     3901 ctatgtgctg tgcaacatcg acggttatta cctgctgtct gaagatattc cggctgccga
     3961 acttatcgcc agtatgaata ccattctcga aaaagcggaa gaactggata ttcaatcgct
     4021 gcctgaaatt caggatatgc gggaaaacaa ctggggggca atcctgctcg aaggcccata
     4081 ctcctgggag atcatgtctg aaattcatgg ctttgacgtg attggcctgc cttactacga
     4141 atacatgaat accgatgagg atctgctgat cttccgctgt ggtaaacacg gtgagtatgc
     4201 gtacatgacc atcggtgagc aggcgaaact ggcggagcag tgggaaaaac tgttaaccgt
     4261 cggtgagaaa tacctgatgc agaccggcgg tctggattat caaaaaatcg tacgcctcga
     4321 aaatccgtgc tgggatgcca gcctgtggga aggtcagtcg gtgaatccgg ttcagttgca
     4381 gatgcagtgg gcggtgcagt acgacaaaga tgattttatt ggcaaggacg cggtgacaga
     4441 gctctcccag gagtataccg gtaataaact catcggcatg atcgctcagg aagaatgcga
     4501 aggcattgag gctggcgatc gtgtgctggt ggaaggtcaa gacgtgggct atgtcgtgaa
     4561 agcggtattt tccccggcat tgcagcgttt cattgccctg accctgctgg aaaaggatta
     4621 cgcctggtct gatatcagcg gctacgaaat tcaaactgcc cacggaatta ttccggcgca
     4681 atcaaaatgt atgccgttta tttataacct gagcatgttg gttagcccaa ctgagcacag
     4741 ttatatcgac gcgtcaaaaa ataaaagcgc agcgtaatga atatcgccgc gtgactatag
     4801 cttacgcggc ttaaattaac cccaattggc gggattgcca ggtattattc atgaaaacga
     4861 acaggaaaag agttgttatt accgggctgg ggattttgtc ctcactggcg gaaaatattc
     4921 aggatttccg ggaagcatta ttaaataaaa aaaatggcgt tgcggatagc gaacgctttg
     4981 caaaatggtt tgaaaatgcg cgtgcggcag aagtgttgca tgacattgaa tactccgatc
     5041 tgaacgatga tgttatcgat tcactggata acgcagcgct ctgggcctac aaagtgggta
     5101 aagatgcgct gagtcaggcc ggcctgatgg atagccgtga caccctcaaa gaaacaggca
     5161 tgatcgtcgg tgtttcctcc gcgggtacag aggccttttt accgctgttc gaacagcgca
     5221 tggaagattt ctcgctgcgt aaagcactct tttccggcgc attctcatcc tgttgttcca
     5281 gcgtttcaac gctgctgggc ttacaggggg gggttgaact ggtcgcgacc gcgtgtaccg
     5341 ccagcccgaa cgccgtgggg atggcctttg actacatcca gaatggtaaa agcaaaacca
     5401 tgctggcagt aggtaccgag cccatctatc tgccgacctt tgccggattc tacgcactga
     5461 atgtcatgca tccagacact tgtacgccgt tctcgggtca gtccggtatg tcgattggtg
     5521 aaggtgcggg cgctatcgta ctggaagagt atgagcatgc agtagcccgt ggggcgacca
     5581 tttatggtga gatcctctct tacgccactt cctgcgatgc tttccatgag accgggccag
     5641 atccgcgtgc cagcggtgcg gtgcaggtca tgaacaaggc aatggcaaat gcgggcgtca
     5701 caccggagca aatcgactac gtcaacgcgc acggtacggg tactgaagcc aatgaccgta
     5761 tcgaaacgct ggcaatgaaa aaggtctttg ctaaccacga taaactgctg gtgagctcca
     5821 ccaaatctta ctttggccac aacattggcg cagcggggat tgtggaactg attgcctgtc
     5881 tggcaacact gccggacaac cgcgtactgc cgacgctgaa tttcagccag gcacgaccga
     5941 attgcgatct ggactatgtg ccgaatgaat ttcgcgatcg caaagtgaac atcttcatga
     6001 agaataacta tgccttcggc ggcaacaact gctgcatgat cctgagcatg gagcccgcgt
     6061 ccattccggt cagtacctat gattcgaagc gtgtggctat ctcgggcgtc ggtgcagtat
     6121 ccgcaattgg ccacacgctc aaccaggttc tggaaaacat ctgggcgcag gaccaccacc
     6181 ttgaactggg tagcgtaacc ttcccgtacg atacactgga agaagccaaa gagctgctgg
     6241 ccgtgctgga tgaatcaaac cagtttgccg agctgtttga tgaagagtac agcttcagcg
     6301 acgctacgct tccggaaacg gcaagcaact tcaaaacgtt ccaggtttcc gggctggagc
     6361 cgcgtaaaca cctgcgtcgt ttcgatcagc gtaaagcaac ccgtggcggc acgtttgccc
     6421 tgatcgcact caccgaagcg ctggaacagg cgaagcgtaa aatcaaacgc gacggcgatg
     6481 agctgggcat gatcatgggg atgtcccgcg gcccgcagga aaccacctac aaatatctgc
     6541 aaagcctgaa acccgatcca cgcaaagtac gtacgtcgga attccctggc tcactgatga
     6601 atgcgatccc aacgttctgc gggatctctg aagggatcaa agggtatacc actacgcttg
     6661 cgacagggga aaactcggcg ctgggcgcac tgacctacgg ttatgaaatc gtccggcaga
     6721 acctgcaacc gcaggtattg gttggcggcg cggatgagta cttcccgtcg atgtcgctgt
     6781 atatggatgc ggtgacacaa aagatccaca tgacggctga ggcgagcgac tatcaaattt
     6841 acggtaaaga tccgcagggt tatgttccgg gcgagggtgc ctgcatgctg ctgctggaag
     6901 atccacagtt ggctgcggcg cgtggcgctg aggttctcgc ggaagtggtg ggctacggta
     6961 agtcatgtag taatacctac ttcgacgcct cattgttgga agaaaaatcc gcgtcgatgg
     7021 cgctggccat tgaacgcgcc cttaagcatg cgggcatctc tgctggcgat attgatttgg
     7081 tatgtggtac cagcaacggc agcgatgacc attctcgtat cgaaattgac gccatttaca
     7141 acaccttcgc cgcgcaaaac ccggcggtgc cggtagtgaa ctacaacgct tgctttggtt
     7201 ttgtcgcctc tgccgctggt ttgcttaacc tcgccgtgat tactgactgc attaagaagc
     7261 aggcagtacc tgcgattcca aatacggtgg aattctttga tgagcgtgtg aattttgtac
     7321 gccagtcgat gaaacttgcg ctgaaccatg tcctgcttgt tggcgcaacg gaaggcggca
     7381 actactacgc gttcgtcatt aagggataag actgtggaca atgccgctat tatttcgggt
     7441 tacagcgtgt ggatgccgta tgccgaagac tgttcgcagt taattgacaa tctgaaacag
     7501 gggaaacgcg ttgcgcgtac accctggttt acctccaacg aggaagccat taaatgcggg
     7561 tttaagggta atccgtcagt ggcgacgttg aaacaggtgg acgatagcgc attggacctg
     7621 ctgtctcagt tgatcgacga agccctggaa caggcaatgc tggataagca ttgcctggcc
     7681 gggcgcaacg ttcgcgttta cctgaccggt attgggccgc gtatcgacgg gctggattac
     7741 aaatctttct ataattacaa cgatgtagaa gatatcaact taacgcaatc catcaccaat
     7801 ctgcatgccg cgaagatgtc gcaggacacg atttcaagcc atcttgcgcg caaatatcgc
     7861 ttgcagtatt tgccgcctaa catgaactgc accagtaact cgtcactgac tgcggtgcat
     7921 ctggcgacgc agggcattga gcagggcggg attgatctcg cgattgtgct gaactgttcg
     7981 aaaattaaaa cccaggatat ctggttcctg gaaactcaat cgatgctgga cagcgaacag
     8041 gttcagccgt ttggtgaaaa cagtaaaggt gtggcgttcg cagaaggttt tagcgcgctc
     8101 ctactggaaa gcgctcatca ccgccgggcg cgtcaacaaa gtgagggcgt aagggtgcaa
     8161 acgacttaca cccaaatcag cgcgggccgc agcaacgatg cctcctggct tagcactaac
     8221 gtgcacaagg tcatgcaggc cgcgatgaag caggctgaaa ttgcgctgga tgatcttgca
     8281 gccattcttc cacacggcaa cggttcggca gtgagcgata acgcggaagc caaagccatc
     8341 accatgttcg caggggagcg gcagatcctc gttctcgcct ataaagggca gattggctat
     8401 accgcaaccg gatctggtgt tgtcgatctg atcattggcc accattcgtt gacgcatcat
     8461 caactgatcg cacctgttgg taacgacgtc attatcgaca gcatggcttc gctggtactt
     8521 acggacggca gcgtgacaaa ccatagcaaa cgccatttgc tgaaggttgg tgtgggtgtc
     8581 gatggttcag ttattggcgt tgtcatgtca aatacgcaag cggggcgcgc gaaatgacgg
     8641 aaaagagtat ctacctgtct tcctgggcgc ttacggaacc ggacgacatt ttttattttt
     8701 atcagccgac ctggctcaac gcctgggaag cacatatgta cgaaagagga ttatatggca
     8761 ctcgtgctaa tggctggggg ctgaagcgct ttggcgttga gaacgatatt tatgcgccgg
     8821 aacggatccg taatccacaa tatatgcctt accttgaggc gatggagcag gtaaccgatt
     8881 ctgcattaca acaaatgccg actaaagagc gcatgaagtt aatgaaatcg cgcacggcat
     8941 ttatttatgc cgattcctgg ggggaatccg gtccgtttga aaaaatttcc agtgcgctgc
     9001 atatcgccac tattgatacg cttcccaaga atttacttaa gaagttttcg ataacggaac
     9061 cgacttgcaa gattcgcggc gaaaaacagg cattcgttca ggcgctgcgt atggcgcagg
     9121 attatctcgc ctgggatgta ttcgataacg tggtaatttg tgctgcctat cgggcaattc
     9181 cggtgctggt attctctgaa gaagatgtgg gtaaaccgcc taagcgttta tttccccgtc
     9241 gtgctgatga ggtgaattta tctgttgaac gagtgggttg ttttattctc agccagcgtg
     9301 agagcgctat caggctgcaa tgcggggaat atcattccgg cactgcgcca cagcatgcta
     9361 cggatattga tgtcatggcc ttcagcgaac ggcgacaaat gccgtcccgg cctgctgctg
     9421 gcgttatcga cctggtagcc cattacggag aaagcggttg tctgacgccc gcgctgagta
     9481 tggattattt aacccgtcat attcagcccg gtggaaaaat gcgtacggtc attgccgata
     9541 aacagtttgg ttatcactac tttgaccttg aataccttga aggataacga cagtcgtatg
     9601 caatctacta ttaaatcgtt gcccgctatt tcgcttagca acatttacaa gtgttatggc
     9661 gctggggcag gaaaggttga tgcgcttaaa agcattaatc ttgatatcaa taaaggcgaa
     9721 ttcctcgcac tgtgtgggcc gtcaggcagt ggtaaaagta ctctgttgaa tattctctcg
     9781 ggtattgata aaccgaccag cggaacggtg atatttttaa ataaattact gaaccaactg
     9841 ccagaagagc agttggctgt gattcgtgga aaacatttgg gttttatttt tcagtttttt
     9901 aaccttatgc cggtattaaa tgttttcgat aacgtttatt tcccactggt gcttaatggt
     9961 cagttcagta aaaaagaagc cactgaacgg acgctgcatt atctcgatag tgttggatta
    10021 agcggttttg gcgatcgtaa acctgggcaa ctatcgggcg gtcaacaaca gcgtgtcgcc
    10081 atcgccaggg cactggccca cgaaccgatg gtggtcattg ccgatgaacc gacgggaaat
    10141 ttagacctgg caaccggcga ggctattttg gacctgttgt tgacgattaa tcagcagacc
    10201 ggcaccacat ttgttatttc cacgcactcc agcgaattaa aagcgcgggc acggcgagtg
    10261 gttgaaattc aggatggagt attgattcat gactcagatg cataaaggat ttttgctcat
    10321 cggcgtcttt ttgtacgcgg cgtttgtggc tccgaagatt tacgcggcaa cccccgacgc
    10381 tgttaaacag gttctgcaac aggggctccc ggcgcagcct gccgcgctgg cgacgctgac
    10441 cgaggcgtta aagaaagagt atgaagccag gcacaatgtg gtggcgctgg ttttttacgc
    10501 ctatggctta ctgcgtcaag cggacggtta tgcggcaaca aacgatttta tccatgcctc
    10561 agaatacgcc aaaagcggct ttttctggct ggatgaagcg gtggatttgc aggagaaaaa
    10621 tcaacgcgta cggtatttac gcgcccgcgt agatgcctat ttacccgcag actcagggcg
    10681 ttgcgtggtg acagtcaaag acaccgaaca catgctggct gacccggcga tatggacgac
    10741 aacgatccgc gaccatattc tggcgatgcg ttatcgcgca ttacgtcact gcaaggacac
    10801 cacccgcgcg aatgcgttgt tggcgcaaat taaagggcaa aacgccgcgc ttgcgcagtc
    10861 gctgacgcag gattttaatg ttgtatcaga atgggatagt gaagagttaa cccaggtact
    10921 gctgccgctg atgaaaggtg agtaaatgtc cggggaaatt ttagctgtta atagcgttct
    10981 gctgctgatc ctgttttttg tgttttatca gcttctgttt tattcacgag atgtcaaatt
    11041 tgcatttttg aatctctttc gccacaaacg tcgctctttt tctaccataa ccgcaattgt
    11101 attgggtggt gtggcgatct ttttatatgg cggatttatt gattactctt tctggatcct
    11161 gaaagagcag acgattcgta ccaatattgg tcatgtgcag atttacaatc agcactattt
    11221 tgaaacgtcg aataaaaata agagcctgat tgccgattat gcggcgttaa agaaagctat
    11281 tttgtcggac ccgacgctgg cggcggatat ttccacgctc tccgggcaac tggagtttac
    11341 tggcgtaatt tcacattacg agagcgaaac ttccagctat ttctccgcgc tgggggtgga
    11401 gccgttaccc gctttaaagc tgggttcgtt cgataagatt atcgcgggga gcgatctttc
    11461 tcgggtaaaa ggtgatgaaa tcaccctggg cagcggtctg gcgaaaacgt tgaatgcgaa
    11521 atacgacgac tggctggatg tgatggtggt caacacggcg ggcgggcagg gcgcgttgtc
    11581 gctaaaactg cgtggcattt ttgagtcagg cattaaagat tatgacgatg tcgcgatgaa
    11641 gatcccgctg gacaccgcgc agcgcatgat gggtacggat ggcgtcagca aagtgctaat
    11701 tctgcttaaa gaagatgaca ccgcggcgtt tagcacaaag ctgcgtcagt ttatcgaacg
    11761 caatcatttg ccgctggtgg tcaaagactg gcgtgatgtt tcactctttt atcaacaggt
    11821 tgaagggttg ctatcgggga tctatttctt catcaagctg attgttgcgc tgattgtcat
    11881 cttcatgatc ggcaattcga tggctatgaa cattgttgag cgcacccgtg aaataaccac
    11941 tctgcgtgcc ataggcctga agccgcttca cgtcacgcgc ttgtttctga cagaaggcat
    12001 ttttattggt gtgattggcg ccgtcggcag cctgctggtg gggggcgtgt tagcctggat
    12061 tattaatctt tatggtattg cgatgccgcc atcgcctgga caaaccatcg gctataccgc
    12121 ctttataaaa accagtaacc cggaattgat ttgggtgacg gtggtattac ccattttgac
    12181 agcaacaggc gcctctgtgt taccggcttt acgtgcttcc cggctgaata tttctgacgc
    12241 atttaaattc tcatagggct aattatgaat atggcttttt acggcaagtg gtttgcttgc
    12301 ctgtggctcg ctacgtcgtg cgttcaggcg gcgagcacag ataataaggc gctggagata
    12361 attcgtcgcg ctgacgaaat tcgttcgccg aataaaccgt ttcgttacac gctgacggta
    12421 acggaatata aagcgggcgc aacccagcca gagaataaac aggtcctcga tatttccatg
    12481 cgctttatga aaccgcaggg taatgaaaaa gcggatgcac gctcgctggt gcgttttatt
    12541 tatccgccgc gagataaagg aaaaatcatg ctttccgact ggtacgattt atggttttac
    12601 acgccggaac tgcgacgtcc tatgcctatt tcacggcaac aaagattaat tgggcaaatc
    12661 tctaacggcg atgtcattgt cactaacttt gaatatgcct atgactcgac cctggtgggg
    12721 gaagtcacgt gtgctgaaaa gcagtgttac aagctggcgc tggtgcgtaa atcggcggat
    12781 atcacctggc cgaaggttat ctattacgtt gaaaaagacg gtgataatcg cccgtggaag
    12841 gcggcctatt attcgcagga tgaccagtta ataaaagaag tgttgtatca ggatttccag
    12901 ccggtgttgg ggaaaacgcg acccatgaaa ataaccgtta ccgatgttcg tcacggcaat
    12961 aactattcag tgatggagta cagcgatgtt cgtctggaat cgctgccgga gtttcatttc
    13021 accaaagagt atattcagcg aggtgcgaaa tgaaacgtac gctgaagatc tcttcgttgc
    13081 tgtgtgttgc gctgccgttg acggtacagg cggattgcct gtcaggtgac gaggtggcac
    13141 aaaacagcga tgtgacgctt tacgagcagg tgtcttatgt caataaacag gcttcggcct
    13201 ggcagattgc aggcaaaaat ccctacactc gtcacaacgg ttatcaggag gcggggattg
    13261 gcatcaacag tggttgttcg ataatcgaca atacgctgga ccttaagctc aatctgtatg
    13321 gaataaacga atatgcgctt aaacccgcag ggaaattcga aacggatgat agccgcacac
    13381 gtgcgttaat taaccgcctc agtttggtct atagcgcctc ggacagcgtt caatttgaag
    13441 caggcaaatt tgccgcgccg tcaggaatgt tctttttgcg ttcaccttct gatttgcaaa
    13501 cgcattatta caccggattc cagtcgacac gtttgcacga tcccaaaatg acatcggcgt
    13561 atcaggcgtc atcgtggggc gcaaaaatga acttggatac acgcgattat gcgttttcgg
    13621 cgagcgtcat accgaagtta gccaccatcg acaaacgcta tgtgacgtct ggcaactggt
    13681 ctgctaatca gcagggaaac agcgacgaag cctatctgct gagctacagc gatcatcgct
    13741 ttggtgagca cacaccgaca gtcaatgtgc ggctcggacc atcgccgtca ctcgcgctgt
    13801 ctgacagtta tcactacacc ccgcagctga cgctgaatgt cgacgcagcc taccaccgct
    13861 cccaacaatg gcggcatctt tcccaccatg agacggcgca ggtcgaggaa tatcagtttc
    13921 cggattcgct ctatgaaacg aaagatgaaa gcggcgtgga gctggcgctg ggtgggcagt
    13981 acaccagtga taatttcagc gtgtttggcg ttgagtatta ctttcagagc gagggttatt
    14041 cgcgcgctga acagcgtcag cagcgcgagt taattgactt cctcaatacc acaaccggct
    14101 atgcgccact ggatcaggca tttgattctt acaaatacct gatggcttca gaaatcagca
    14161 ataccgccaa tcaggggatg ctgcagggta agcactatct gaacgcctgg gccagtctgc
    14221 cgctggcggg agagtcgacg ctgcagcctt cgctggtggt caatctcatt gatggcagca
    14281 cgttgctggg cctgcattat tccacgccgc tcagcgctat cagtaaccag cttgaagcct
    14341 atgccggtgg ctatagcgcg ctgggaagtc ggtattctga attttcgctt tttggcgaca
    14401 cgttaggcct ttatttgggc tttaaatact atctataagg ataaagagat gatgaataat
    14461 gcttcacaac cgacagtact gctttttgcc ggtcagggta atccggttat tggcatgggc
    14521 agcgatcttt gggatctcaa cgccagtaca aaacatatct gggattgtgc cagcgatatt
    14581 tccgggctga acctgcgtcg cctgtgtcag aaggggccga tgaatcgcct tattcaaacg
    14641 acggtacaac aactggcggt gaccgcaatc aatgtaacgc tctataccct gtgccgtgaa
    14701 cgtcttgaga atttaacggt tgtggggtcc tgcggacaca gcgttggtga atacagtgcg
    14761 ctctacgctg ccggagctat ttctcttgag acgctatttg gcatgattca cttcagagcc
    14821 agcctgatgc atgacgaaag ccagagaaat aaaggcgcaa tgctggcggt taaaggcatc
    14881 aaccgcgaac aactgcagac gatgattacg gaatcaggca ttgcgctgga tattagctgt
    14941 gataacacgc cgcgccagca ggttattggg ggaacgcagg ctgcgttgaa tgaatttgcc
    15001 acgctgctga tggccgctgg atacgaaccg gttaaactcg gtgtgagcgg tgcctggcac
    15061 acgcgtttga tggaagacgg cgttcaggca atgcgcgatt atcttgccgg actggatatc
    15121 gcttcgccag agcatcaggt tctgatgaac gtaacagcaa aaagcgaggt cgctccgtca
    15181 attatcaaag aaaatctctc attacactta actcatacgg tgaaatggac tgaatctctc
    15241 gatacatttc tgaatatgcc aacctcggta gccttccttg aaataagtaa taagccctat
    15301 ttggggaata tgttaaacga ttttgctggt gtggatcagc aacgagttat gcattgtcgt
    15361 aaagcattta c
//
LOCUS       DAZKFU020000106        15359 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZKFU020000106 DAZKFU020000000
VERSION     DAZKFU020000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.107"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1857)
                     /gene="traD"
                     /locus_tag="ACHOQQ_RS13215"
                     /old_locus_tag="pgaptmp_002643"
     CDS             complement(<1..1857)
                     /gene="traD"
                     /locus_tag="ACHOQQ_RS13215"
                     /old_locus_tag="pgaptmp_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HGU4470874.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDNILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDKKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLMAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKSAASLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQA"
     gene            complement(1913..2644)
                     /locus_tag="ACHOQQ_RS13220"
                     /old_locus_tag="pgaptmp_002644"
     CDS             complement(1913..2644)
                     /locus_tag="ACHOQQ_RS13220"
                     /old_locus_tag="pgaptmp_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470875.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYSPVIQDNTATTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLVMYFINSLRHKELTEILTKISFPEKSGSNPLKPEEGRC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAKEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDLINKTSRVSPDSGHFYEPGTALDYRERVRLFVQIQYSHESDWSEILRLTDIV
                     LEKVSSGRPAGGYHHRYTGYTWFVL"
     gene            complement(2812..3072)
                     /locus_tag="ACHOQQ_RS13225"
                     /old_locus_tag="pgaptmp_002645"
     CDS             complement(2812..3072)
                     /locus_tag="ACHOQQ_RS13225"
                     /old_locus_tag="pgaptmp_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470876.1"
                     /translation="MNRAESRLSQVLFLLGYDSEKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            complement(3132..3863)
                     /gene="traT"
                     /locus_tag="ACHOQQ_RS13230"
                     /old_locus_tag="pgaptmp_002646"
     CDS             complement(3132..3863)
                     /gene="traT"
                     /locus_tag="ACHOQQ_RS13230"
                     /old_locus_tag="pgaptmp_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HGU4470877.1"
                     /translation="MKTKKLMMVTLVSSTLVLSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQTWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSKVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3895..4398)
                     /locus_tag="ACHOQQ_RS13235"
                     /old_locus_tag="pgaptmp_002647"
     CDS             complement(3895..4398)
                     /locus_tag="ACHOQQ_RS13235"
                     /old_locus_tag="pgaptmp_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HGU4470878.1"
                     /translation="MIMKNLAHVALITVIQLIACNLADWGGAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLFSPLLSLMTFIIGTLYELRRVCGCV
                     SVREWMQSQVNENTNEELHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            complement(4414..7257)
                     /gene="traG"
                     /locus_tag="ACHOQQ_RS13240"
                     /old_locus_tag="pgaptmp_002648"
     CDS             complement(4414..7257)
                     /gene="traG"
                     /locus_tag="ACHOQQ_RS13240"
                     /old_locus_tag="pgaptmp_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HGU4470879.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASSDPYTIIFSQPSPLRQVPNNNYSFLQSSEPFVSCK
                     DASVGLKDKLNFDTNTGGKTWNYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSAS
                     QIMRQNVTMNALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVLMRNLPM
                     VQTILVGITIGIFPLLVLAAVFNKLTLSVLKGYVFALMWFQTWPLLYAILNSAMTFYA
                     KQNGAPVVLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVRGLGAGFSSVYSHFASS
                     SITPTASAAGSVVDGNYSYGNMQTNNVNGFSWSTNSTTSFGQMTHQTGSGATATQTRD
                     GSMVMDASGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLS
                     QFGSNRGSSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISDEQATRELASRST
                     RGSAGMYGDAHAEWGVKPKILGVGGGAGVRGGGRTSIDWNDEDAHQASSGSRASHDAR
                     HDIDARATQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTN
                     MTRSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRR
                     AMAWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQ
                     DSNIRNDVKQQVDNMVTEYRGNIGDTQNSIHGEENIVRGQYSELQNHHKTEALSQNNK
                     YNEEKSAQEGMPGADSPQELMNRAKEYQDKYKQ"
     gene            complement(7254..8627)
                     /gene="traH"
                     /locus_tag="ACHOQQ_RS13245"
                     /old_locus_tag="pgaptmp_002649"
     CDS             complement(7254..8627)
                     /gene="traH"
                     /locus_tag="ACHOQQ_RS13245"
                     /old_locus_tag="pgaptmp_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HGU4470880.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFATNTTQ
                     AGVWQGQAAGYAYGGSLYARNQVRNIQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMANDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(8614..9006)
                     /gene="trbF"
                     /locus_tag="ACHOQQ_RS13250"
                     /old_locus_tag="pgaptmp_002650"
     CDS             complement(8614..9006)
                     /gene="trbF"
                     /locus_tag="ACHOQQ_RS13250"
                     /old_locus_tag="pgaptmp_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HGU4470881.1"
                     /translation="MKEKRNDAEQKNRKTKRDYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPKLLNFHYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRRDNKLREGGNNASH"
     gene            complement(8987..9273)
                     /gene="trbJ"
                     /locus_tag="ACHOQQ_RS13255"
                     /old_locus_tag="pgaptmp_002651"
                     /pseudo
     CDS             complement(8987..9273)
                     /gene="trbJ"
                     /locus_tag="ACHOQQ_RS13255"
                     /old_locus_tag="pgaptmp_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
     gene            complement(9263..9807)
                     /gene="trbB"
                     /locus_tag="ACHOQQ_RS13260"
                     /old_locus_tag="pgaptmp_002652"
                     /pseudo
     CDS             complement(9263..9807)
                     /gene="trbB"
                     /locus_tag="ACHOQQ_RS13260"
                     /old_locus_tag="pgaptmp_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
     gene            complement(9794..10084)
                     /gene="traQ"
                     /locus_tag="ACHOQQ_RS13265"
                     /old_locus_tag="pgaptmp_002653"
     CDS             complement(9794..10084)
                     /gene="traQ"
                     /locus_tag="ACHOQQ_RS13265"
                     /old_locus_tag="pgaptmp_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HGU4470882.1"
                     /translation="MISKHRFSLPRLDITGMWVFSLGVWFHIVARLVYRKPWMAFFLA
                     ELIAAILVLFGAYQILDTWIARVSQEEREALEVRQQLLLRERKDNDDEHVSR"
     gene            10166..10501
                     /locus_tag="ACHOQQ_RS13270"
                     /old_locus_tag="pgaptmp_002654"
     CDS             10166..10501
                     /locus_tag="ACHOQQ_RS13270"
                     /old_locus_tag="pgaptmp_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HGU4470883.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSFPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFILWSVVINLTIGFVIYDK
                     LAPVISLMK"
     gene            complement(10482..10823)
                     /gene="trbA"
                     /locus_tag="ACHOQQ_RS13275"
                     /old_locus_tag="pgaptmp_002655"
     CDS             complement(10482..10823)
                     /gene="trbA"
                     /locus_tag="ACHOQQ_RS13275"
                     /old_locus_tag="pgaptmp_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HGU4470884.1"
                     /translation="MHTGDLCFFTGASLFIVGGVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCVLPDNFLFLCGAVMNQCAVPVVSMAVGTIPVITKFERFRRIFVCIMLSSAWSG
                     VMWFFIISLVK"
     gene            complement(10837..11580)
                     /gene="traF"
                     /locus_tag="ACHOQQ_RS13280"
                     /old_locus_tag="pgaptmp_002656"
     CDS             complement(10837..11580)
                     /gene="traF"
                     /locus_tag="ACHOQQ_RS13280"
                     /old_locus_tag="pgaptmp_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HGU4470885.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVLAA
                     PRQEPDIMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIARLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11573..11830)
                     /locus_tag="ACHOQQ_RS13285"
                     /old_locus_tag="pgaptmp_002657"
     CDS             complement(11573..11830)
                     /locus_tag="ACHOQQ_RS13285"
                     /old_locus_tag="pgaptmp_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HGU4470886.1"
                     /translation="MKVIFTSNRLVDFLIRLVITAIVISPVIIWSWDTVKETTAGGVL
                     AAAFIILYSGVLLFILYGCFSALADLQKPDERKSDERNEDE"
     gene            complement(11844..13748)
                     /gene="traN"
                     /locus_tag="ACHOQQ_RS13290"
                     /old_locus_tag="pgaptmp_002658"
     CDS             complement(11844..13748)
                     /gene="traN"
                     /locus_tag="ACHOQQ_RS13290"
                     /old_locus_tag="pgaptmp_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HGU4470887.1"
                     /translation="MKEKINTFFRSLNVNDSGRPRNICAIVFFLSFSVFADSNSDYRA
                     GSDFARQIQGQGIGSIQSFNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWA
                     TSETGKTITESFMNKPKDILSPDAPFIQIGRDVVNRADSIVGNTGQQCSAQQINRSEF
                     TNYTCERDTMVEEYCSRTARITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPV
                     AGTVESASLSAHAGLYFLNSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLT
                     GSGCTANGNCLPHGNGDRMVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLKGTECSEPGGTKTGVMEGKTWSITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGACLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKSPDCRGITVDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQ
                     IAGQLKQVGQ"
     gene            complement(13842..14222)
                     /locus_tag="ACHOQQ_RS13295"
                     /old_locus_tag="pgaptmp_002659"
     CDS             complement(13842..14222)
                     /locus_tag="ACHOQQ_RS13295"
                     /old_locus_tag="pgaptmp_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470888.1"
                     /translation="MSLPAGYYRIEPDIRALVAAMNVHGFRTYASCQGHGFPVTKLLP
                     YIAFACPVKMTALLEQRLRQDAESAIPRLTWGWSVKGAFNSDLQLCFRLQPEGPHCWY
                     HRYCRRSLRADFRTLILLLKSLSE"
     gene            complement(14219..14857)
                     /gene="trbC"
                     /locus_tag="ACHOQQ_RS13300"
                     /old_locus_tag="pgaptmp_002660"
     CDS             complement(14219..14857)
                     /gene="trbC"
                     /locus_tag="ACHOQQ_RS13300"
                     /old_locus_tag="pgaptmp_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HGU4470889.1"
                     /translation="MNKKIYILFALTAGLFLSAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHADNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKLQ"
     gene            complement(15086..15358)
                     /locus_tag="ACHOQQ_RS13305"
                     /old_locus_tag="pgaptmp_002661"
     CDS             complement(15086..15358)
                     /locus_tag="ACHOQQ_RS13305"
                     /old_locus_tag="pgaptmp_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470890.1"
                     /translation="MRLTGKALKLFPFALVMLFTVLFYFMPENVICDIISEWQRSDVS
                     DRIRVIRLYLGTTFLCTWAAVFLEFLCSDEFLPGKRLNKNEEENNG"
BASE COUNT         3828 a         4106 c         3460 g         3965 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaaacaac ctccgggaca tccggttcga agaggctggc
       61 catctgacga ccttctgctt cccttgcggc aagtacggca ctcagacggt tctccatttc
      121 aggattgatg tcacgcggaa taaactccgg agcaaccttc ggtcgtgcct gatatttcag
      181 ggagagtttt actgccggat acggtccggg cagggtgaca taacaggtca gatccggcag
      241 agactgaatg tcggaataac tgaccagcgt ctggcgctcc atatccttac cggtcgatac
      301 cccgtcacgt accggatcag caccgtagga atactgctcg ctggctttca ggtgctcttt
      361 ctcaccaatt tcaccggcag cgaactctgc aatcttatgg ctgggggaac ggaaaaaggc
      421 acgggtgttc atgacgtcaa acagcgaggc tgccgatttc tcaccgtaga tatcttccag
      481 ctgggcatag gactggatac cgaacacata acagccaccg aacttacggg cttccggcag
      541 gatctccacc aggtccggca gtttgtgtaa cgtgggtaac tcatcacaga agaaccacac
      601 acgacggtta cggttctccc ccatcgccat caggccacga atggcgatgg acagccacat
      661 ggagatcacc ggtttcaggg aagcatgggt gtcggcattc gacgaaataa acagccagcc
      721 gtttttctta tcttcacgga caccccgcat ccagtcacgg atggtgaagg actcaccatt
      781 atgctcaatc ccctgcaggt aacggatggc tttcacgtag ttggtcagca cagcacggat
      841 ggaaatcgcc gttttctcga ttttctcttc caccaggttg gccgccggtg agttacgcag
      901 gaaggtacgc agtttttcaa ttttgatgga gagtagtgta tccaccagtt tgctgtagct
      961 gcggttgggg tcattacgca tcaggtacgc cgcttccgca aaaatggtgc gtcctgagcc
     1021 ctgccagaac gggtcttctt ttgtgcccat cgggatcagg gtatttgccg tattatcaaa
     1081 atccggctgt gtcaggcact ccttccacaa atcccaggcg gcacagcggg catccagcgg
     1141 attcaggata ttatcgatgg acgggtcata gtaactttta acaaattccc ctgatcggtc
     1201 ataaatcacc accatatcac cgcgctgacg ggcgtagttg gccagacgac ggataacttc
     1261 cgacttaccg gccccgaccg tgccgtgcag acagaaattc tggatttcag aatcccggat
     1321 aatcggcaga tcgccaatcc ggatatcgga gtccttgccg tcttttttca gcatcctggc
     1381 aacctctttc ggattgtcag tcagctgacg accacctgtg acttcatttt cactctgctg
     1441 tttaccctga cgccccagaa tccaggagac aacaaagaag gtgatcaggc aaataaccag
     1501 ggccacgaca gacgccagaa cgaatgcaga ccatagctgc tcaccgcacc agatcatgta
     1561 tttatcatgc agtacctgag ccgcgttcat ccggaaggtt ttgccgtaat actggatctc
     1621 atagaccggc tgggacctga ttaaatcccg catgccttcc agcgtggtgc accaccagta
     1681 aatacagccg ttcacaaacg tctgccagct tatttttacc cataaaacca gaccaacgag
     1741 gatccagaaa aaaataaaca ggcagtaaag catgatattg gcgatctggc tgaacatgcg
     1801 gatacgcatg gacgcaatct gaccgccctg ggtcatatcc tttgcgttaa aactcatgat
     1861 gaaactccgg taaagtcagg tacagagcag aaaagagcgg tgtggaatac attcacagaa
     1921 caaaccatgt ataccctgta taccggtggt gatagccgcc tgctggtctg ccggaagaaa
     1981 ctttttccag cacgatatca gtcaggcgca atatttcaga ccagtcactc tcgtgtgaat
     2041 actggatctg aacaaaaagg cgtacccgct cacgataatc cagagccgtg cccggttcat
     2101 aaaagtggcc actgtcagga gaaacacggg atgttttatt gatgagatcg tatttatcgg
     2161 catgagtctg tgacgacaga gaccaggtaa tattttcttc ctgctcacca tcaatacggc
     2221 tactcccggg cgtccaggca atttccttcg cactggtcac gatgatctgt ctggagactt
     2281 ctgccagttt tttgggggta cagaataccg gggccagcac acacagggac aacaaattac
     2341 agcggccctc ttccggctta agcggattgc tcccggattt ctcaggaaaa gatatttttg
     2401 tcagtatttc agttaattcc ttatgcctga gagaattaat aaagtacatg acaagcatca
     2461 tgagaaacaa tataacaata agccctccga taaccactaa aaggtcagta tcaatattat
     2521 tgctgtacag ataatctttt gtggtcgctg tattatcctg tatcaccggg gagtattcag
     2581 ttacaggctt ctcagtaaat tgattattct caccaacata ttcagcgata ttgtctgatg
     2641 tcataatgac tttctccgga agcttccggc ttcgccggca acacttcacg gtcgcacaaa
     2701 gaatttgcac aaccgttcag tgttctcata tttatttcgg aaacgccggg aagtgacaaa
     2761 atttattttg ttactttccg gggttggaca acgagcaacg cgaggcgtca gctattttct
     2821 tctgcgacgt ccggtcggat attcactcac aggaagatat tctgttgcgc ttacgccgat
     2881 ctcatatgca gagaacggtt ccagtgctgt caggtatctc tggcaggcta tggccagttc
     2941 agaatctccg gttgcatcag caaaccggta aagttctttt aataactccc gacatacatg
     3001 ctcaggatac ttttcactgt catagcccag cagaaagagt acctgcgaga gacgagattc
     3061 tgcacggttc atgtattcat ccggggcagg aatatcggcc cggtacaatc tgtccgggcc
     3121 ggatgggtaa tttacaggat gttcgcgatg gatttagcca gctggtcttc cagtacaggc
     3181 ttcgcttctt caaatttcag gttaaccttg ttggcattag ataccacacg ggtctggtat
     3241 ttatgttgat ttccggttga tgtgctggtc tgcacttttg caccggaggt tccctggcgg
     3301 agagcagcaa cattatcagt tctgacttta gatgttgtcc gctctgcaat ctgcacatcc
     3361 gtgatcatgg tgtagttgat atcctccacc attgcatcgg cagccatacc aatcaaccct
     3421 gtggcaatcc ctacacccag cgccgcaccg gcagagctgg agttatatcc tgtgatgccg
     3481 gtgcccaatg ctgcaccggc aatggcacct tcataaccgc tgttcagcca ggtctgggac
     3541 tcccgcaaat ccattttgtc tgccttcagg acattggcct ggatccagta gtatgccttg
     3601 tcaggtgagg tcacaacctg gtagcctttg gctttaacgg catcactgat ttttgcctgc
     3661 aatccactca tatccttgtc agatgtgttt ttgatttgca ggaaaacggt gcgttcactg
     3721 gaaggctcca gccagatggt ttcactcatc tgagttttga cttcaagatt acgcttcttg
     3781 attgctgtgc tcatcgcacc acaccctgaa aggaccagag tggaactgac cagggtaacc
     3841 atcatcaatt tttttgtttt catcaaatta cctttattac aagctacagt tttcttatcg
     3901 cctatcatca tagtcctgcc aacaagtccc aaaagtattt cctgctgaat caaatcctcc
     3961 atgcatagct aatcctgttg ccggattagt acgataaaaa tccacataat caaaattcac
     4021 atcatcaaaa tccagatgca attcttcatt ggtgttttca tttacctgag attgcatcca
     4081 ttctcttaca cttacacaac cacaaactcg ccttaattcg tatagtgtac caatgataaa
     4141 agtcatcagg cttaataacg gagagaataa agaagatact ggcatcagta tataccaaac
     4201 gcctttactg aatttaaact catccgacac atgccgtttt tttcgtattt gagaaaacaa
     4261 ccatataaac aatccctgcc atagtacaat aaagcacagg ataaaaactg tctcagcgcc
     4321 cccccaatca gccagattac aggcaatgag ctgtatcaca gttatcagag caacatgagc
     4381 taaatttttc attatcatct cccaaaccca gcattattgc ttatacttat cctgatactc
     4441 ttttgccctg ttcataagtt cctgtgggct gtcagcaccg ggcattcctt cctgagctga
     4501 tttttcttca ttatatttat tattctgaga taatgcctct gttttatggt gattttgcag
     4561 ttcagaatac tgccctctta caatattttc ctcgccgtgg atgctgttct gagtatcccc
     4621 gatattgccc cgatattctg tgaccatatt atcaacctgc tgcttcacat cgttacggat
     4681 attactgtcc tgcgttcttt gctcaataat accctgatgc ccctgatggt ctgcaataat
     4741 atcctggctg ccaccgcccg aaggtacgct ctccattcct ttaccgatat cgccacggga
     4801 ttcacgccag gcgttatcaa caccaggctg tacctgttcc tgaacaaaag accaggccat
     4861 tgcccgacga cgttctgcaa tctccggtga actggtgttt gtcagaatag cttctgcatc
     4921 ctgcggcgcg tgtttcatca catactgtgc aaactgttgt gacaggtctt cactcatctg
     4981 cccactcatg ctttcagtac gggaggccat ttcagcatat tcatggctgc gggtcatatt
     5041 cgtcgtgtac tggtcgtaac tctgtttcgc tgagttcagg gctgcagaaa gctggtccac
     5101 cctggaatcg gcattattgt cagtatggct gccggattca ctgaccttgc gactggtaaa
     5161 gtaatcgctt gcttctttaa agtcttgtgt agctctggca tcaatatcat gacgggcatc
     5221 atggctggcc cgtgaaccac tgctggcctg atgcgcatcc tcatcattcc agtcaatact
     5281 tgtcctacca ccacctctga cacctgcgcc accaccaaca ccaagaattt taggtttaac
     5341 cccccattca gcatgagcat caccatacat ccccgcagaa cctctcgtac ttctggatgc
     5401 cagttctctt gtcgcctgct catcgctgat attatgcgcc ttcgcatagc tttccaccgc
     5461 actgcgcata cggctggcca tcatggagtc ctgcgcgctc atcgtgctgt cagcgccact
     5521 ggtgactgaa tcactgcttc cccggttaga gccaaactgg ctgagtgtgt tccaggcact
     5581 ggcaatactg ctgctgaaac catgcagggc actttccgcc ttattcatcg actcccgggc
     5641 catttcctgt tgcgctgctg caatctgacg cgttgcatta ataccaaccg gcaaacggga
     5701 cattgcccca ctggcgtcca tcaccatact gccgtcacgg gtctgcgtgg cggttgcacc
     5761 actaccggtc tggtgcgtca tctgaccaaa tgacgtggtg ctgttggtgc tccagctgaa
     5821 gccgttcacg ttattcgtct gcatgttgcc gtaggagtaa ttaccgtcaa ccacactgcc
     5881 tgccgcactg gcggttggcg tgatggaaga agacgcaaag tggctgtata cgctggaaaa
     5941 tccggcacca aggcctcgta ccatcatcca tgacagaggg gggatcattg ctgaaagata
     6001 acctgcggtt gatgccaggt cggagtattt cagctgtatc tgagagagtt cagagagcac
     6061 gaccggagcc ccgttctgct tcgcatagaa tgtcatggca ctgttcagga tggcatacag
     6121 caacggccag gtctggaacc acatcagggc aaacacatag cctttcagga cggacagcgt
     6181 cagtttgttg aagaccgcag ccagcaccag gagcgggaag ataccaatag taatgcccac
     6241 cagaatggtc tgtaccatcg gcaggtttcg catcagcaca tgaccaatgg agacatgtgc
     6301 cagacgctgt ttctccatcg atgaggtggt ggccagattc accaggctgg cggtatcacc
     6361 gttacgggcc gcattactgg tgataccctc tttcagggca ttcatggtga cgttctggcg
     6421 cataatctgg ctggcagact ggctggagcc gtagaaataa ctgtaactgt cactcaccag
     6481 ttgtctgaat aacaggtcag ggtccggcct gccgccaaat atctgctgca cataataatt
     6541 ccaggtcttg cctccggtat tcgtatcaaa attcagctta tccttcagtc cgacggatgc
     6601 gtccttacag gacacaaacg gctctgagct ttgcagaaaa ctgtaattat tattcggcac
     6661 ctgacgtaaa gggctgggct gagaaaagat tatggtgtac gggtcagagg aggccatcag
     6721 gtcttccagc gtgtatttat ggttcaggta aatatcaccc agcacgcagt tctggacgta
     6781 gtcctgaaac agattgatga tttccggatt ccgggacagg aaatcggtgc ttttcacaat
     6841 caggttcgcc ccgaacagca tgcctgtctt gctgtaggtg gcgctgtccg gctgagcgaa
     6901 caccatctca tagccggcca ccatcgcatg cccgatacgg gtcgtcagtg aaagtggcat
     6961 cgccagaccg accggcacat tgtccacccg gtgaacctta accaggtcac tgttatcaat
     7021 aatctgcacc gatgtgcgga cattaaccag caggctgata agcacaaaca cggccaccca
     7081 gcccagcaaa tccatcacgt tgtgccgttg tacccacatc acagccactg cgagaacaga
     7141 taatgtgagc gcaatttttt caatggaatc ccacgtccgg gtccccataa aagcggcaat
     7201 ggcgttcagg ttattgcgca accactcacc accggcaatc acatagactt cattcacagc
     7261 gtgctccctc cgaagtgata gttgttctgg taacggctga gcatacgggc ggacagctgc
     7321 tggcgcatgt agctcatctg acggtcaaca accagcagtg catcctgctg gacctttacc
     7381 tgcccctgga aaaccgcaat ctgttgctgt gcctgattca gattctccag cacactgtcc
     7441 agcacggtct gtggatagtt cccggtggca atcatggccc gggcctgctg aagcagttcc
     7501 tgaatgtact ggagcatgat gtcgtagccg atatagtccg tcagctggta aaccacgctg
     7561 ctggacacac cgagcatctg cgggtcaata agatatttaa agacggggac ggtggtgctg
     7621 gaaatgaagc ctttttccct gtcatccagc ggagtgtcac tcacggcttt attctgaata
     7681 ctggtgagca gttttgtaat ctgtgatttc agggctttat cccgggcgat ggtcacattt
     7741 gcatccgcca ccaccttcag acacttgtcc gaatcgttgc agtggtaaat ttttgccgtt
     7801 cccccctcca taatcgcccg gaggatatcg cggtcagtgg tacgcgccgg caggggggta
     7861 atctcgctgt cttcaccaaa aatcagcgta cctgtgagcg tcataatgaa ctctttcagc
     7921 tctttgttac catcaagcat cctgtttttg gacagcgagt cccagatgat atttatgttc
     7981 ttcagcaccc gttccttatc tttgtcactg gccttgtcac gaacagacga tgactgcccc
     8041 ccaaccgtgc agccctgacg ggacgcggcc cagtcggcga aaatattgct ctcacctgca
     8101 atatcctgac agaccttctg ctgtgacacc tgagtctgag gaaataatcc tccgacaatc
     8161 ccctgtgcca cctgacagga gctgaggttc atactgttaa tgtcattcgc catcttctgc
     8221 aggaagtctt tcgcggtttt gatttcgggc accgttgtct gcagggcaag gtcaaaaaag
     8281 taccccatcg cgttactcat aatctgctta acaaaacgct gcagctgctc gccattaata
     8341 aaactgaatg agccgagata ggcatcgatg ccaccacacc ctgcattgat gtcgggcagt
     8401 gtcatggaga taagctggat atttctgacc tgattacggg catacagaga accgccatag
     8461 gcatacccgg cagcctgccc ctgccagact ccggcctggg tggtattggt ggcaaagccc
     8521 agcttgttga agaactggtt catgtcgctg ttcacatccg ctgacgctgc aggtgtgacc
     8581 gtcagtaaag cggcacaaag aaccagaaga ggtttaatgc gaagcattat tgccaccctc
     8641 ccttaatttg ttatcccggc gcatacggcg tttcagccgg aatatcctga tccccccgtt
     8701 tattaattca tttaaagggt tcctgacgta tacgataaga aatccacctg caaacgccca
     8761 taacgtatac ggaatgacat acatcaggat gttccacata tagtggaaat taagtaattt
     8821 cgggtccgct ttccacgaat caatacaggc atcaaaaata ctgtgcataa tcacccataa
     8881 aaaagttccg gcagcaacga acacaaaaaa cagatcaaaa tatatatcag aaaccctgcg
     8941 ttcataatca taatcccgtt tagtttttct gtttttttgt tccgcatcat ttcttttttc
     9001 tttcattata agtttcctct gatatttctg atgattcatt cagaccgttc atgcggctgt
     9061 aatatatttc agatactgaa atccatccca caattgcccc cacaaaaaca caacagaaac
     9121 agaaaaggta agacacagcg aaacgacaat ttctgcaggg ctggcgggat aaaaaagtcc
     9181 ctgacggata aaggggatat tcaggctgac gattattccc gtcgcgacgc cggatattcc
     9241 ggcaatgagt aacaccacct gcttatttcg cacctttttt ccctccgtac atctgcagaa
     9301 cggtatccat ccgcgccata aaaccggcgg catccgttgc ccctgtaaaa gcggtaatgc
     9361 ctcaagcgtg ttgacgttga ccaggaaggt ggtcggtgtg gccaccggga tattcgggaa
     9421 gaatgttttc agtacatccg gtggtgcagg taacgcctgc ggaaaggccg tatcaccctg
     9481 accatccagg gtatagggaa atacggaaaa tccgtactgc agcgccagtt gtttcagtac
     9541 cgggtcaaac tggtgacagt acggacatcg cccttgcatg aacagcacca ctttccagtc
     9601 agcaagattg acctgagttc cgttgctgag gcggaaccag cgggttgctg ctttctcctg
     9661 tcgcacctgt gcgtctgctg ccggctgagt tgtccgtgct gccattccct ccggatgcca
     9721 gagtcgctcg atttccgcac gtgtggatgc ctgtgtacag ctggccagaa aaagcaggac
     9781 aacggacagc gatttaacgg gaaacatgtt catcatcatt atccttccgt tctctcagta
     9841 acaactgctg tctgacttcc agtgcctcac gctcttcctg actgacgcgg gctatccatg
     9901 tatccagtat ctggtatgcg ccaaacagaa cgagaatggc tgcaataagc tctgccagaa
     9961 agaaagccat ccagggtttt ctgtaaacca gacgggcaac aatatggaac cagacaccca
    10021 gggaaaatac ccacatcccc gtaatatcca gtcggggtaa agagaatctg tgtttactta
    10081 tcatttgaat atcctgttgt ggtttacatg aatttattca cgtatataac ctgcttaact
    10141 ttcgtgaaat taatcagtgt gttatatgaa aaagttatct ttcaaagaaa aactggaaat
    10201 agtgagaaac atcataagag agtcattatc aggtaatgcg gcttttgctt tactggtgta
    10261 ttcattattg cattccttcc ccattaaaac ctttgctgat ttatttgtga cgatactgtt
    10321 atttatagca atcatcgctg ttgtgatatg gctatttcta atcatctatg tttatttttg
    10381 tgaattattc agaagtcact ggatagcagt ctggtttatc ctctggtctg tggtaatcaa
    10441 cctcactatt ggatttgtca tttatgacaa attagcacca gtcatttcac taatgaaata
    10501 ataaaaaacc acattactcc tgaccatgct gatgacaaca taatacagac aaatattctt
    10561 ctgaacctct cgaacttcgt gataaccgga atggtaccga ctgccattga aacaacgggt
    10621 acggcacact gattcataac cgcaccacaa agaaacaaga aattatctgg cagcacacat
    10681 acacttatga catttactac aaagaagaca aacgagctca gcagataaac tcctttatta
    10741 tttggtcctg taaagaaata accaacaccc cccacgataa acaggctggc acctgtaaaa
    10801 aaacacaaat cccctgtatg catatatatc ctttggttaa aaattcggtt taaaatcttc
    10861 agatacgttc aggaactgtt ttgccaggtc atcctgcgaa ataaagccgt atgataatgg
    10921 acgaacactg ccctgtttcg ggtctaccag catcatggcc gggaaatatt tcacgccgag
    10981 gcgctgcgcc tgcccctggt cagtccggga atccggcaat agtggattaa tcacgccatc
    11041 cacggaaacg ggaataacac tcagaccata cgtatcccgg aagccattaa tgacctgcgc
    11101 cagctgcccg tcgatggggt cctgcccccg gtaaaaaaac ataatgccgt aatgctctgc
    11161 cagtctggca atcgcctgtc gctgctgcgc ctggtctgct gccagctggt tccgtaccgt
    11221 gccgttgtaa tggctgtact gcaggttata gtccagttca ggatgtgcca gcatggcctt
    11281 tttggcgctc atggtgaaaa gcccggcctg ctgagtccag taattctgca gccggaaata
    11341 tttcacaaaa ttatccacgc cgggatacag aatggcttcg tacagcgccc gctttgttgc
    11401 cgtctgcagc gcggtcagtt tctgcataat atccggctcc tgacggggag ctgctagtac
    11461 tggtttattt tctttttcct tcggatttat tttctcgtta taccactgcc agcctgcatc
    11521 tttccctgac gccggaaaaa taaagcagca gagtaacagc ggcagtaatg ctttattcat
    11581 cttcattcct ttcatcactt tttctttcat caggtttctg caggtcagcc agtgcagaaa
    11641 aacaaccata cagaataaac aggagcacac cggagtaaag gataatgaaa gcagcagcaa
    11701 gcaccccgcc tgccgtggtt tcttttaccg tatcccacga ccagataatt accggggaaa
    11761 tgacaattgc tgtaataaca agccggataa gaaaatcaac aagacgattc gacgtgaata
    11821 tgactttcat tattttcccg accttactgc cccacctgtt tcagctgacc ggcaatctgc
    11881 tctttcactt tttcggtcag cgcaccatta tccgggattt tctggttatt catcaggtct
    11941 tcgtagaagt tagtgaagtc cagctggtcg aacttaatac gctgcagttc atccacggtt
    12001 attccccggc agtcaggact ttttgcgctg ccaaacccga tacgcaactg cccgttacgc
    12061 ccctgctgct ggactatctg cgccagcttc gaatcaaact ggcagtaact gcgctttttc
    12121 tgcagacaga cacccagcac tttccgggag cagaactcgc ccacactcac cgtcagtttg
    12181 ttgtcctttg cctttcccag cgccttttct tcgctgttgc acttcgccaa acccacatcc
    12241 tgaccccagc caccatcctt acagcagtta ctgaagccgg ctgcggcctt acggcagaat
    12301 ttcgcctcgc ccgtgaaagc cctgacatcc acaccattca gggcggcaac atccttacct
    12361 gcggcggcaa gcgcagccag ctgtgatact gcctcaccaa aatcactgct ttttccgctc
    12421 tgcgccttgt cgcattcacc gtcaaggcag aagacatcac caccacagac catcactttt
    12481 ccggacgttc tggactcgca ggagtacgtg gcatactcat gcagacaagc tccctcatcg
    12541 gaataaaacg cgcactgacg tgaggccagc gtacaggccg gattatcgac atatttctga
    12601 caggtgccgt tatctgcgga ctgtgtgaca tatttatccc ggtatgccca gcacgcctgt
    12661 gtgatgctcc aggttttacc ttccatcaca cccgttttcg taccacccgg ttctgaacac
    12721 tcagtgcctt tcagcacgcc atccgcttta ttaaacggac aactctccac ccactccacc
    12781 cgtggaaccc attccttatc acggaccttc atcctgagtt tcagcgtaaa ggtggaagca
    12841 ccactgacaa gcgattcata gaccatcctg tcaccattcc cgtgcgggag gcaattaccg
    12901 tttgcagtac agccactacc ggtcagaacc tgcccctgtg tgacagaaaa cccggagggc
    12961 acaggtatgg tgaaagtccc gctctgtcct gtaaccagtt gcacattaaa ggctgtgttc
    13021 ataaagtcgt aacgggagtt aaggaaatat agccctgcat gagccgacag cgaggcactt
    13081 tccactgtcc cggcaacagg tacggtaacg ctgaacacca gtttcagacc gttcatactg
    13141 aaccggaact gtgagtgcgg tatcgtcacc tcacgatatt catccgtgta tttccagtct
    13201 cctgtgatcc tggctgtcct ggaacaatat tcctccacca tcgtgtcacg ttcacaggta
    13261 tagtttgtga attcactgcg gtttatctgc tgcgcactgc actgctgtcc ggtatttccc
    13321 acgatactgt cagcccggtt caccacatcc ctgccgatct gaataaacgg cgcatccggt
    13381 gacagaatgt ctttcggctt gttcatgaat gactctgtga tggttttgcc ggtttcactg
    13441 gttgcccatt gtgtggtgcc gttatttttc agaccgccat ccccgccggc agtcacccca
    13501 ccgtaatact gcgtctcatc cgggttcgcg ttatacccgg ggatactctc ctgcggatta
    13561 aaactctgaa tactgccaat accctgcccc tggatctgac gggcaaaatc ggaacctgcc
    13621 cggtaatcac tgttgctgtc ggcaaataca gaaaagctca ggaaaaaaac aattgcacaa
    13681 atattgcgcg gtcttccgga gtcattaaca ttcagagacc tgaaaaaggt atttatcttt
    13741 tctttcataa cctgatccat actggagaat gaagtgatgt catctcaact tcagaacggt
    13801 gttgattttg gtgtatggag cttcctggcg taccgggatt actactcaga taacgacttc
    13861 agtaacaata tcagcgtcct gaagtctgca cgcagtgaac gacgacagta ccggtgatac
    13921 cagcaatgag gaccttccgg ctgaagccgg aaacagagct gaaggtcact gttaaaagcc
    13981 cccttcaccg accatcccca tgtcagacgt ggtattgctg attcagcatc ctgccgcagt
    14041 cgctgctcaa gaagggcagt catttttacc ggacaggcaa aggcaatata aggcagcagc
    14101 tttgtgaccg ggaaaccatg tccctgacag gaggcatatg tacggaatcc gtgaacgttc
    14161 atcgcggcca ccagtgcacg gatgtccggt tcaatccggt aatacccggc aggtaaactc
    14221 attgcagctt atccccggtg ccgtccagca gtccctgagc cacctgccgg cagtctcccg
    14281 tggcggccac tttttccagc gcctgcctga cgcgcagatt tccccggatg atgtcgtgcc
    14341 cctggctgca gaacaccacc agggccggca cgctgcggat gccgtactgt gaaaacaacg
    14401 tcgggtcgat ctgaacacca tcggtcgcgc catcttttac cagggacaac acggcttcag
    14461 cggtagtctt caggtcattg ttcaccatac cccgcagtgt ggccggaatg ccgtagtgcc
    14521 tggtttctcc cagcatccgt ttcagtccct cttcgggaat ggagaacgac acaaaataca
    14581 cggccccctg ctgtggtttg tctttctgag cagcctgctg tttacggacc agttcatcga
    14641 gaaactggtt atcggcatgc tgaaggggat tcgccctgac ctgctgttct gcccatgcct
    14701 gcagttgtgt gtccggcttt tcacgcaact gctgactcag tgtttcctgc tgcttcagga
    14761 actggcgatt ttcgggcgta ttcacatttt ccgaagcatg ggcagaaaga aacaatcctg
    14821 ctgtcagggc gaataaaatg tatatttttt tgttcatata attctcctgc aaacagacag
    14881 taattaatcg tcataatgag gtgctcaata gagcaagcct ggagataact gacgccatgt
    14941 taaacacaac gaagatcgtt gattaacttc tttctccatt tatctttttt ctgagactgg
    15001 cagcaattag ataaacgata ccggatattc actctgttaa atcattaatt catttctgac
    15061 cgatgcacat aaaatgagtc agatattacc cgttattctc ttcttcattt ttgtttaatc
    15121 tctttcccgg cagaaactca tcagagcaga gaaactccaa aaaaacagcc gcccaggtgc
    15181 ataaaaaagt tgttcccaga tatagtctga taactctgat cctgtctgac acatcacttc
    15241 tctgccattc actgataatg tcacagataa cattttcagg cataaaataa aacaacaccg
    15301 tgaatagcat caccagtgca aaaggaaaca attttaatgc tttgcctgtc aaacgaatg
//
LOCUS       DAZKFU020000107        15349 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZKFU020000107 DAZKFU020000000
VERSION     DAZKFU020000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.108"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            189..989
                     /gene="ydcF"
                     /locus_tag="ACHOQQ_RS13310"
                     /old_locus_tag="pgaptmp_002662"
     CDS             189..989
                     /gene="ydcF"
                     /locus_tag="ACHOQQ_RS13310"
                     /old_locus_tag="pgaptmp_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGU4470891.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTIFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPHVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            1186..2625
                     /gene="aldA"
                     /locus_tag="ACHOQQ_RS13315"
                     /old_locus_tag="pgaptmp_002663"
     CDS             1186..2625
                     /gene="aldA"
                     /locus_tag="ACHOQQ_RS13315"
                     /old_locus_tag="pgaptmp_002663"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU4470892.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISSGIRECASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2667..3668)
                     /gene="gap"
                     /locus_tag="ACHOQQ_RS13320"
                     /old_locus_tag="pgaptmp_002664"
     CDS             complement(2667..3668)
                     /gene="gap"
                     /locus_tag="ACHOQQ_RS13320"
                     /old_locus_tag="pgaptmp_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4470893.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVNGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3857..4387
                     /gene="cybB"
                     /locus_tag="ACHOQQ_RS13325"
                     /old_locus_tag="pgaptmp_002665"
     CDS             3857..4387
                     /gene="cybB"
                     /locus_tag="ACHOQQ_RS13325"
                     /old_locus_tag="pgaptmp_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGU4470894.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4632..4805
                     /locus_tag="ACHOQQ_RS13330"
                     /old_locus_tag="pgaptmp_002666"
     CDS             4632..4805
                     /locus_tag="ACHOQQ_RS13330"
                     /old_locus_tag="pgaptmp_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4470895.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4831..4899
                     /gene="yncP"
                     /locus_tag="ACHOQQ_RS13335"
                     /old_locus_tag="pgaptmp_002667"
     CDS             4831..4899
                     /gene="yncP"
                     /locus_tag="ACHOQQ_RS13335"
                     /old_locus_tag="pgaptmp_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HGU4470896.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4877..5026)
                     /gene="hokB"
                     /locus_tag="ACHOQQ_RS13340"
                     /old_locus_tag="pgaptmp_002668"
     CDS             complement(4877..5026)
                     /gene="hokB"
                     /locus_tag="ACHOQQ_RS13340"
                     /old_locus_tag="pgaptmp_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGU4470897.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            5425..7065
                     /gene="trg"
                     /locus_tag="ACHOQQ_RS13345"
                     /old_locus_tag="pgaptmp_002669"
     CDS             5425..7065
                     /gene="trg"
                     /locus_tag="ACHOQQ_RS13345"
                     /old_locus_tag="pgaptmp_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGU4470898.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRVGGMFMIGAFVFALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITTVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVLAEPRGAGKPVSFATV"
     gene            complement(7104..8027)
                     /gene="ydcI"
                     /locus_tag="ACHOQQ_RS13350"
                     /old_locus_tag="pgaptmp_002670"
     CDS             complement(7104..8027)
                     /gene="ydcI"
                     /locus_tag="ACHOQQ_RS13350"
                     /old_locus_tag="pgaptmp_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4470899.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            8244..9587
                     /gene="ydcJ"
                     /locus_tag="ACHOQQ_RS13355"
                     /old_locus_tag="pgaptmp_002671"
     CDS             8244..9587
                     /gene="ydcJ"
                     /locus_tag="ACHOQQ_RS13355"
                     /old_locus_tag="pgaptmp_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4470900.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            9812..11467
                     /gene="opgD"
                     /locus_tag="ACHOQQ_RS13360"
                     /old_locus_tag="pgaptmp_002672"
                     /gene_synonym="mdoD"
     CDS             9812..11467
                     /gene="opgD"
                     /locus_tag="ACHOQQ_RS13360"
                     /old_locus_tag="pgaptmp_002672"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGU4470901.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            11607..11831
                     /gene="ydcH"
                     /locus_tag="ACHOQQ_RS13365"
                     /old_locus_tag="pgaptmp_002673"
     CDS             11607..11831
                     /gene="ydcH"
                     /locus_tag="ACHOQQ_RS13365"
                     /old_locus_tag="pgaptmp_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGU4470902.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            11894..12433
                     /gene="rimL"
                     /locus_tag="ACHOQQ_RS13370"
                     /old_locus_tag="pgaptmp_002674"
     CDS             11894..12433
                     /gene="rimL"
                     /locus_tag="ACHOQQ_RS13370"
                     /old_locus_tag="pgaptmp_002674"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HGU4470903.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(12425..13405)
                     /gene="ydcK"
                     /locus_tag="ACHOQQ_RS13375"
                     /old_locus_tag="pgaptmp_002675"
     CDS             complement(12425..13405)
                     /gene="ydcK"
                     /locus_tag="ACHOQQ_RS13375"
                     /old_locus_tag="pgaptmp_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGU4470904.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGKSR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            13529..14521
                     /gene="tehA"
                     /locus_tag="ACHOQQ_RS13380"
                     /old_locus_tag="pgaptmp_002676"
     CDS             13529..14521
                     /gene="tehA"
                     /locus_tag="ACHOQQ_RS13380"
                     /old_locus_tag="pgaptmp_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGU4470905.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFIARLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            14518..15111
                     /gene="tehB"
                     /locus_tag="ACHOQQ_RS13385"
                     /old_locus_tag="pgaptmp_002677"
     CDS             14518..15111
                     /gene="tehB"
                     /locus_tag="ACHOQQ_RS13385"
                     /old_locus_tag="pgaptmp_002677"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGU4470906.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFAGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3914 a         3580 c         3874 g         3981 t
ORIGIN      
        1 aattcaggaa ttcagaaaaa tacaattctc tgctgcaaga tgaataatgt ttatctacag
       61 catttcctta aaagatatgt caggcttgcg gagtggcggt taaggaaata cgacatcctc
      121 ctttcagagt gctccgcttc tcactattat ctcacgcagt attcttaagg gaacgataag
      181 gaggaaccat gaacattacc ccgtttccga cgctttcgac ggcaactata gatgccataa
      241 atgttatcgg acagtggctg gcgcaggatg atttctccgg tgaggtgccg tatcaggccg
      301 attgcgtcat acttgcaggc aatgcggtta tgccgactat agatgcggca tgtaagattg
      361 cccgcgatca gcaaattcct ttactgatta gtggtggtat cggtcactcg accatttttt
      421 tgtatagcgc catcgcacag catccgcact acaacactat ccgcaccact ggcagagcag
      481 aagcgaccat cctggcggat atcgctcatc agttctggca tattccgcat gaaaaaatct
      541 ggattgaaga ccagtcaaca aactgcggtg aaaacgcacg ctttagcatc gcgctattga
      601 atcaggccgt agaacgagtt catacggcta tcgttgtaca ggaccccacc atgcagcggc
      661 gcacgatggc aacgttccgc cgtatgaccg gggacaatcc ccatgtacca cgctggttaa
      721 gttatcccgg attcgttcct cagttaggaa ataacgcaga cagtgtaatc tttattaatc
      781 agttacaagg attatggccg gttgagcgtt atctctcact actcactggc gagctgccgc
      841 gtttacgcga tgatagcgat ggctacggtc cccgcgggcg agattttatc gttcacgttg
      901 attttccggc agaagtcatt caggcatggc aaacgcttaa acacgatgcg gtgctcatcg
      961 aggcgatgga aagtcgctcg ttacgttaaa aattgcccgt ttgtgaacca cttgtttgca
     1021 aacgggcata actcctgcct tttatttctg cctcttattc cttttacact tgtttttatg
     1081 aagcccttca cagaattgtc ctttcacgat gccgtctctc tgatgattga tgttaattaa
     1141 caatgtattt accaaaaaca aacatataaa tcacaggagt cgcccatgtc agtacccgtt
     1201 caacatccta tgtatattga tggacagttt gttacctggc gtggagacgc atggattgat
     1261 gtggtaaacc ctgctacaga ggctgtcatt tcccgcattc ccgatggtca ggccgaggat
     1321 gctcgtaagg caatcgatgc agcagaacgt gcacaaccag aatgggaagc gttgcctgcc
     1381 attgaacgcg ccagttggtt gcgcaaaatc tcctccggga tccgcgaatg cgccagtgaa
     1441 atcagtgcgc tgattgttga agaagggggc aagatccagc agctggctga agtcgaagtg
     1501 gcttttactg cggactatat cgattacatg gcggagtggg cacggcgtta cgagggcgag
     1561 attattcaaa gcgatcgtcc aggagaaaat attcttctgt ttaaacgtgc acttggcgtg
     1621 actactggca ttctgccgtg gaacttcccg ttcttcctca ttgcccgcaa aatggctccc
     1681 gctcttttga ccggtaatac catcgtcatt aaacccagtg aatttacgcc aaacaatgcg
     1741 attgcattcg ccaaaatcgt cgatgaaata ggccttccgc gcggcgtgtt taaccttgtt
     1801 ctggggcgtg gtgaaaccgt tgggcaagaa ctggcgggta acccaaaggt cgcaatggtc
     1861 agtatgacag gcagcgtctc tgcaggtgag aagatcatgg cgactgcggc gaaaaacatc
     1921 accaaagtgt gcctggaact ggggggtaaa gcaccagcta tcgtaatgga cgatgccgat
     1981 cttgaactgg cagtcaaagc catcgttgat tcacgcgtca ttaatagtgg gcaagtgtgt
     2041 aactgtgcag aacgtgttta tgtacagaaa ggcatttatg atcagttcgt caatcggctg
     2101 ggtgaagcga tgcaggcggt tcaatttggt aaccccgctg aacgcaacga cattgcgatg
     2161 gggccgttga ttaacgccgc ggcgctggaa agggtcgagc aaaaagtggc gcgcgcagta
     2221 gaagaagggg cgagagtggc gttgggtggc aaagcggtag aggggaaagg atattattat
     2281 ccgccgacat tgctgctgga tgttcgccag gaaatgtcga ttatgcatga ggaaaccttt
     2341 ggcccggtgc tgccagttgt cgcattcgac acgctggaag atgctatctc aatggctaat
     2401 gacagtgatt acggcctgac ctcatcaatc tatacccaaa atctgaacgt cgcgatgaaa
     2461 gccattaaag ggctgaagtt tggtgaaact tacatcaacc gtgaaaactt cgaagctatg
     2521 caaggcttcc acgccggatg gcgtaaatcc ggtattggcg gcgcagatgg taaacatggc
     2581 ctgcatgaat atctgcagac ccaggtggtt tatttacagt cttaatgagt gaaagaggcg
     2641 gaggtttttt cctccgcctg tgcgcgtcag agtttagcga atttttcgag ggtgcgaata
     2701 agctgcgtga cgaagccata ttcgttatcg taccaggcga ccgttttcac cagttgtaaa
     2761 tcgcccacgg cggtaatttc cgtttgcgtg gcatcaaaca ccgaaccgaa atggctgcca
     2821 atgatatcgg aagagactat ttcttcatcg gtataaccaa atgactcgtt attggtggtt
     2881 gcttgtttaa gtgcgttatt cacctcttcg gcagtcactt tttttccgag aatcgatacc
     2941 agttcagtga ccgaacctgt tttcaccggc acgcgttgcg catgaccttt cagtttgccg
     3001 ctcagttccg ggatcaccag accaatggct tttgccgcgc ccgtagtgtg gggaatgata
     3061 ttttctgccg ctgcgcgtga agcacgtaaa tctttaccac gcgggccatc caccagtgac
     3121 tgggtgccag tataggcatg aatggtcgtc atcgtgccga cttctatccc gaaactgtca
     3181 tgcaaggctt tggccatcgg cgcaaggcag ttagtggtgc atgacgccac ggaaacaatg
     3241 gtatcgttgc catccagagt gtcgtcatta acgttataaa cgatggtttt catttcaccg
     3301 gcaggagcgg aaatcaacac cttcttcgca ccagcatcaa gatgcgcctg cgatttctcg
     3361 gcggaggtat aaaagccagt acattcgaca atgatttctg cacctttcgc tttccacgga
     3421 atatttttag cctctttttc ggcgtaaacc gcgatacttt tcccattaac gataagtgaa
     3481 tcttccgtaa aatcaacgct ccaggggaac ggtccgtagt ttgaatcatg tttcagcagg
     3541 taggcgagaa tttttggaga agtgagatca ttaatagcga caacgtctat gttgcttttg
     3601 acttcaagta atcgacgcaa caccagtcga ccgatacgac caaaaccgtt aataccaact
     3661 ttactcatgg ttttctcctg tcaggaacgt tcggatgaaa attgatcctt tccaggctta
     3721 gaccaggatg gcgggatggg caatctccat tcttacagtg aaacgtaacg tactgaaaac
     3781 gggtgaacaa tatttaatga aattttgaga aaagcccgtt atgttaacgg aaaattatgt
     3841 taaagcagga aatgttatgg aaaataaata ctcaaggtta caaatcagca ttcactggct
     3901 ggtctttttg ctggttatcg cagcgtattg cgcaatggag tttcggggtt tcttcccacg
     3961 tagcgatcgg ccactcatca acatgattca tgtttcctgc ggcatatcaa ttctcgtgct
     4021 gatggtcgtt cgtcttttgt taaggctgaa atacccaacc ccgccgatta tacctaagcc
     4081 aaaaccgatg atgacgggac tggcgcattt gggacatttg gtgatttatc tcctgtttat
     4141 tgcgctgcca gtgattggtt tggtgatgat gtataaccga ggcaacccgt ggtttgcgtt
     4201 tggtttgacg atgccttacg cttcagaggc caatttcgaa cgggtagata gcttaaagtc
     4261 gtggcatgaa acgctggcga atctgggata ttttgtcatc ggtttgcacg ctgcggctgc
     4321 actggcacac cactatttct ggaaggacaa cacacttcta cgcatgatgc cgcgtaaacg
     4381 ttcctgaagg atatttaaag aaaacgcctg tactaaaacc gacccgtggt acaggcgaag
     4441 aatacgggtc tacatcggaa gcgcctatta tatttatttg tatgacaaat aaaaccataa
     4501 tccttgccca tacgtccatc tggcttattt ttaatcaatt cacccgatct ttgatctcat
     4561 caacggtatc aaaataaacc agcgtataac gttcaaatcg aacataagat tcgataaacc
     4621 atggaggtta tatgaaaaaa ctggcactta ttttgtttat gggaacgctt gtttcctttt
     4681 atgccgatgc cgggcgcaaa ccctgttctg gttcgaaagg ggggatctca cactgtacgg
     4741 caggcggcaa atttgtctgt aatgatggtt ctattagtgc atcgaaaaaa acatgcacta
     4801 actgaagtgt aaaaggggtg ccatgagaaa atgaatctgc tagtcagatg cgcggggaaa
     4861 atccccgcgc ttgcccttac ctggacgtgc aggccatgag cgcagcaacc tccttatcac
     4921 cgtcccggaa ccgcagttcg tagagcgttt gtcgggtcag gagtgtgaat gtcagaatcg
     4981 taatgcagat aatgagcaga cacaccacca gagggttgtg cttcatagcc tttctccttg
     5041 ccggatggcg ggtaagaggc taagatctga attgctaggt tcattcgttg gcctcggttg
     5101 atagaaatat cggtcggggc cttcgtcttt ctgattcccg gttagcctga aaacagaaag
     5161 tctcaggcac ccgtgagcat cctatgtggt ttccttaggg acgaaaataa tcacctgacg
     5221 aaatttcgtg ctgttttcca gaatttttcg tcattcgggt tagccagttt aaccattcgt
     5281 tactctcttc attccaatgg cattaatctt ctctgcaata attgttgtaa aaatgtgacg
     5341 caaagaggct tttagtcgta agtaattacc gtcaaatgcc gatgactttc tatcaggagt
     5401 aaacctggac gagagacaac ggtaatgaat acaactccct cacagcgatt aggttttttg
     5461 catcacatca ggttggttcc gttatttgcc tgcattctag gcggtatctt agttctattc
     5521 gcattaagtt cagccctggc tggctatttc ctctggcagg ccgatcgcga tcagcgtgat
     5581 gttactgcgg agattgagat tcggaccggg ttagcgaaca gttcagattt tttgcgttca
     5641 gcccggatca atatgattca ggccggggct gcgagtcgta ttgcggaaat ggaagcaatg
     5701 aagcgaaata ttgcgcaagc cgagtcggag attaaacagt cgcagcaagg ttatcgtgct
     5761 tatcagaatc ggccggtgaa aacacctgct gatgaagccc tcgacactga attaaatcaa
     5821 cgctttcagg cttatatcac gggtatgcaa cctatgttga aatatgccaa aaatggtatg
     5881 tttgaagcaa ttatcaatca tgaaagtgag cagatccgaa cgctggataa tgcttatacc
     5941 gatattttga acaaagccgt taagatacgt agcaccagag ccaaccaact ggcggaactg
     6001 gcccatcagc gcacccgcgt gggtgggatg tttatgattg gcgcgtttgt gtttgccctg
     6061 gtcatgacgc tgataacatt tatggtgcta cgtcggatcg tcattcgtcc actgcaacat
     6121 gccgcacaac ggattgaaaa aatcgccagt ggcgatctga cgatgaatga tgaaccggcg
     6181 ggtcgtaatg aaatcggtcg cttaagtcgt catttacagc aaatgcagca ttcactgggg
     6241 atgacagtag ggactgttcg acagggcgcg gaagagattt atcgtggcac cagcgaaatt
     6301 tccgctggca atgcggacct ttcatctcgc accgaagaac aagcggcggc aattgaacaa
     6361 actgccgcca gcatggagca actcactgcg acggtgaaac agaatgcgga taacgcgcat
     6421 tatgccagta aactggcgca agaggcttct attaaagcca gcgatggcgg gcagacggtt
     6481 tccggtgtag taaaaacgat gggcgctatc tccacgagtt cgaagaaaat ttctgagatc
     6541 accaccgtca tcaacagtat tgctttccag acgaatattc tggcactgaa tgctgccgtt
     6601 gaagctgcgc gagcgggtga gcaagggcgt ggatttgccg ttgtcgccag cgaagtacgg
     6661 acactcgcaa gccgcagcgc ccaggcggcg aaagagattg aaggcttgat cagtgaatca
     6721 gtcaggttaa ttgacctggg atcggatgag gtggcaacgg ccgggaaaac catgagcact
     6781 attgttgatg ccgtcgcgag tgtcacacat atcatgcagg aaatcgccgc cgcctcggat
     6841 gagcaaagta gaggcataac tcaggttagc caggcgattt ctgaaatgga taaggtgacg
     6901 caacagaatg cttctctggt agaagaggcc tcagcggcgg cggtgtccct tgaagaacag
     6961 gcggcacgat taactgaggc ggtggacgta ttccgtctga acaaacattc tgtgttggca
     7021 gaacctcgcg gagcgggtaa accagttagt ttcgctacgg tgtgaaaatg ttcaaggaag
     7081 ggatcgacag atcccttcac ctttcagaac ggcattgatt ttcgaatagc gttaatcatc
     7141 aactggcaac cagaagagaa cgtcgcatct acgcgggtca gtattccaat cggttcgcct
     7201 gcaccatgtc ccggaacagg cagggccacc agcgtggcat gacgcaggtc gtcttttaca
     7261 gcgccagaag ggacaaacca cacgtaatcg tattcaaccg taagttgacg ggatagcgaa
     7321 gcagacagcg tttcgataca acccgaagga attttacatc cctggctctg tactaatgca
     7381 tctgaatgct ggcgtggtgc agtgccttct ggtgatacaa cgaccggcca ttccagcact
     7441 cggcttagcg ttacgttctc ctgaagtagc gggtgattag ggcggacaac cagcttcagc
     7501 gattcaagaa acagcagttc gtaattaagc ccggtcatca gttcaggatc tgacatccga
     7561 ccaatgccga tatcgatttc cccggttttt aaacccgcca gaatcatagg gttactcatt
     7621 gtcgcaactt gcaaggtcgt ctctttttgt tgctgatgaa actgacctat aaccgaaggt
     7681 aatatcccca gtgccgcagt aggtagtgca ccaaccctga cgacatcatt attaagacct
     7741 tctttacgat gaagcgactg tccagcagtg ttgatggcgt caagaactct gactgcatgc
     7801 gttaaaaatt gttcgccggg taaggtaagt tgcgccccct gacgaccacg ctcaaacaag
     7861 cgagcgcccg tcagttgctc cagttcattc aatgtcttag agagcgcagg ttgactcaaa
     7921 ttaagggttt cagccgcgcg ccccaaagtt ccttgttgtg cgacagctac gaatgtatga
     7981 aggtggcgca aacggatgcg ctgactaaac agactatttt tttccataag cgatgttaaa
     8041 aacgaagcgg tgtcgctgac aagtgaagtt gtttgattat gataacttga ttgcaaaata
     8101 ttattaacaa ttaaagcaat tatgttacag caaaatggat aatattgatg ttttcgcggc
     8161 gagatcacag tttgtaaatt cttcccgcca gagtgaatgc ggttacctac actccagatt
     8221 actgaccact ggaggcagac actatggcga acagcatcac ggcggatgag attcgggaac
     8281 agttttcgca ggcaatgtca gccatgtacc agcaagaagt tccgcaatat ggcacgctgc
     8341 tggaactggt agctgatgtg aatctggctg tgctggaaaa caatcctcaa ctgcacgaaa
     8401 aaatggtaaa tgcagacgag ctggcgcgac tgaatgttga acgtcatggg gcgattcgcg
     8461 ttgggactgc acaagagctt gctactcttc ggcggatgtt tgccattatg gggatgtacc
     8521 cggtgagcta ttacgatctc tcgcaggcag gggtgccggt acattcgaca gcatttcggc
     8581 ccattgatga tgcttctctg gcgcgtaatc ccttccgcgt ttttacctcc ttactccgcc
     8641 ttgagcttat cgagaacgaa attttgcgcc agaaagcggc ggagattctg cgtcagcgcg
     8701 atatcttcac cccacgttgt cgacaactgt tagaggaata tgagcagcag ggcggtttta
     8761 acgaaacaca ggcacaggag tttgtgcagg aagccctgga aacgtttcgc tggcaccagt
     8821 cagcaacggt agatgaagaa acctatcgcg ccttgcacaa cgaacatcgg ttgattgctg
     8881 atgtggtctg tttccctgga tgccatatca accacctgac gccacgtacg ctggatattg
     8941 accgggtgca gtcgatgatg cctgaatgcg ggattgaacc caaaattctg atcgaagggc
     9001 cgccgcgccg cgaggtgccg attttactac ggcagaccag ctttaaggca ctggaagaga
     9061 cggtgttgtt tgcggggcag aaacagggca cgcataccgc gcgctttggt gaaattgagc
     9121 agcgtggcgt ggcattaacg ccgaaagggc gacaactgta tgatgatctt ctgcgtaacg
     9181 ctggaaccgg gcaggataat ctcactcacc aaatgcattt acaggaaacc ttccgcactt
     9241 ttcctgacag tgagttttta atgcgtcagc aagggctggc atggttccgg taccgtctga
     9301 cgccttcagg tgaggcgcat cgtcaggcta ttcatcccgg agacgatcca cagcccttaa
     9361 ttgaacgtgg ttgggtcgcg gcgcaaccca ttacctatga agatttcttg cccgttagcg
     9421 cggcggggat cttccagtca aatctgggta atgaaacgca ggcacgcagc cacggtaatg
     9481 ccagtcgcga agcatttgag caggcgttgg gttgtccggt tttggatgag ttccagcttt
     9541 atcaggaagc ggaagagcgc agtaaacgtc gctgtggttt gctttaaatt ctgaccatcc
     9601 gcctttgcaa aaatttgcct gattttacaa acgaatcagg ctcatcccat cgacataaaa
     9661 aaattgccga tttatgcata ttctctgagt tcaacaattg gattattaat aaatattgtc
     9721 tagagtgagc ggtcataaat aagcactttc ttgccgctga aaacgaccag cgcgggacca
     9781 ttcacaacac cagaaggact cactttcagg tatggatcgt agacgattta ttaaaggttc
     9841 aatggctatg gccgccgtgt gcggtaccag cggcattgct tctctttttt ctcaggcggc
     9901 attcgcggca gattctgata ttgccgacgg gcaaacccag cgttttgact tctccattct
     9961 acagtcaatg gcgcacgact tagcgcaaac agcgtggcgt ggtgcgcctc gtccgttacc
    10021 tgacacgctg gcgacaatga cgccgcaggc ttataacagt attcaatacg acgccgaaaa
    10081 atcgctctgg cataacgttg agaaccgtca actggacgct cagttcttcc atatgggaat
    10141 gggattccgt cgccgcgttc gtatgttttc tgtagatcca gcaacacatc tggcgcgtga
    10201 aattcacttt cgcccggagt tgttcaaata caacgatgca ggtgttgata ccaaacaatt
    10261 agaagggcaa agcgatctcg gctttgccgg ttttcgcgtg tttaaagccc ccgaactggc
    10321 gcgtcgtgat gtagtatcat tcctcggtgc gagttatttc cgcgccgttg atgacacata
    10381 tcaatacggt ttgtcggctc gcggcctggc gatcgacact tacaccgaca gtaaagaaga
    10441 attccccgac tttaccgcct tctggtttga tacggtaaaa ccgggggcaa ctacctttac
    10501 cgtttatgcg ttgctcgata gcgccagtat tactggtgcc tataagttca ctatccattg
    10561 tgagaaaagt caggtgatta tggatgtgga aaatcacctg tatgcgcgca aagacattaa
    10621 acagctgggc attgcgccga tgaccagtat gttcagctgc ggtactaatg aacgtcggat
    10681 gtgcgacacc attcatccgc aaattcatga ctctgatcgt ctgtccatgt ggcggggcaa
    10741 cggcgagtgg atttgccgtc cgctgaataa tccgcaaaaa ttgcagttca atgcttacac
    10801 cgacaacaac ccgaaagggt ttggtttatt gcaactggat cgtgacttct cccattatca
    10861 ggacatcatg ggctggtata acaaacgccc aagtctgtgg gtggaaccgc gtaacaagtg
    10921 gggtaagggc accatcggcc tgatggaaat cccaacaacg ggcgaaacgc tggataacat
    10981 tgtctgcttc tggcagccag aaaaagctgt aaaagcgggt gatgagtttg cattccagta
    11041 tcgtctgtac tggagtgcgc aaccgcctgt tcattgccca ttagcgcgcg ttatggcgac
    11101 gcgtaccggc atgggcggtt tcccggaagg ttgggcacca ggtgaacact atcccgaaaa
    11161 atgggcgcgt cgttttgccg tcgatttcgt tggtggtgat ctgaaagctg ccgcaccaaa
    11221 aggcattgag ccggtgatta cgctttccag tggggaagcg aagcaaatcg aaattctcta
    11281 tattgaaccc attgatggtt atcgtattca gtttgactgg tatccgactt cggactccac
    11341 tgatccggtc gatatgcgga tgtatctgcg ttgtcagggg gacgctatca gtgaaacatg
    11401 gctgtatcag tatttcccgc cagcgccgga taaacgtcag tatgttgacg accgcgtgat
    11461 gagttaatcg ttttttcttc ggcaccttct tcgggaggtg ccgtctggtt aaacacgatc
    11521 ccgctcgcat ttttccctaa gttaaatgag taatctgatg gtgtgtattt cagatacacc
    11581 ttgtcagcca ctaacaggga gtgcgtatgt ttccagaata tcgagattta atatcccgtc
    11641 tgaaaaacga aaatcctcgc tttatgtcct tgttcgataa acacaataaa cttgatcatg
    11701 aaattgccag aaaggaaggt tccgacggtc gagggtacaa tgcggaagtg gtccgcatga
    11761 aaaaacaaaa gctacagtta aaagatgaga tgctcaaaat cctgcagcat gagagcgtca
    11821 aagaggtgta aactttccta agccgccagc caggcggctt ttttaacaac tgcatggatt
    11881 gactggagat aagatgactg aaacgataaa agtaagcgaa tcacttgaat tacatgctgt
    11941 tgcagaaaat cacgtcactc ctctttatca gttaatctgt aaaaataaaa cctggttaca
    12001 gcagtcgcta aactggccgc agttcgttca gagtgaagag gacacgcgaa aaacggtgca
    12061 gggtaatgtg atgttgcatc aacgcggcta tgccaaaatg ttcatgattt tcaaagaaga
    12121 tgaacttatc ggcgttatct cgtttaatcg tattgaacca ctgaataaaa ccgctgaaat
    12181 tggctactgg ctggacgaat ctcatcaggg gcaggggatc atttctcagg cgctgcaggc
    12241 attgattcat cattacgctc agtctggtga acttagacgc ttcgtgatca aatgtcgggt
    12301 ggacaatccg caaagcaacc aggtcgcttt gcgcaatggt tttatccttg aaggttgcct
    12361 gaaacaggct gagttcctga atgatgccta tgatgatgtg aacttatacg cgcgtattat
    12421 cgattcacaa taaccctgcc agcggcgttc gcgtaatgcg ctcctcgccg ttaatgactt
    12481 ttggaccacg gacatgaacc gtgtcgccat caaaggcttc aacgacggcg tggtcggtta
    12541 actccacatg gttttcaatt atcaccgcac ctgtaatacg gctcttacct tgtattacaa
    12601 cgtgctcgtc gagcagaatc ggtccgccac gtaccacggc atttccgcca atcaagacat
    12661 gatgttttaa cacacaatta ccttccacaa tggcatattc cgccacctgc gaactgtaat
    12721 gaatcgtcgg aatggcatct tcttcaatgc cagctttcac ctgcgcgtga ccgtagactt
    12781 tagcgcaatc gcatagccag acgttgttct cttcattacc ttcaatactg gcaaaatcaa
    12841 aaacttcggc gcgatgttca ataaaagcat accggatgac ggcatcgcca taaatttgtg
    12901 cctggtggac aatacgcgag gcgctaaccc ttgcgcgatc ataaatttgt agcaggagtt
    12961 gatggtcagg cgttaagcct tgtgctgcga cgatcataga atgttgatta attaaggcgt
    13021 gaccaaatat tctacattgc ccgcaaacca gtgagtcatg aatggtgaca ctgtcactta
    13081 tatatgcacc ctggctgatt tcactattat cgatccagac attatccgtt gcgtaaacct
    13141 ctccccacag cacgctggtg ccggtgatac gcgtattacc ggaaatcacc gttccgccga
    13201 acgcaatagc attctggtcg taaatccagc aatttccttc ctgtgctaac accgtttcgc
    13261 gatcgatcca gccgccagcg gttcctgcta tcacatcgtt aaaatcgcta atggcgataa
    13321 tctgccgtaa taacacactt tttttagtgc catcttcctg ataactaaag gctcgctgtt
    13381 cttcacttag acgatatttg cgcatagcgt tttcccacag gtgacttact ataaccgtag
    13441 caaattctgc ggctctggct atgctcgaga aaattccata aaatgcattt caaatatact
    13501 ttataaatta aacaaaatga gtaagaagat gcagagcgat aaagtgctca atttgccggc
    13561 aggctacttt ggtattgtgt tggggacgat agggatggga tttgcctggc gctatgccag
    13621 ccaggtttgg caggtcagcc actggttagg ggatgggttg gtgattctgg cgatgatcat
    13681 ctggggatta ttgactagcg catttattgc ccgactcata cgctttccgc atagcgtgct
    13741 ggcggaagtt cgccatccag tgctgagcag ttttgtgagt ttgtttccgg caacgacgat
    13801 gctggtggcg attggttttg ttccgtggtt tcgcccactg gcggtgtgcc tgttcagttt
    13861 tggtgtcgtg gttcagttgg cttatgccgc ctggcaaact gcgggattat ggcgcggatc
    13921 tcaccctgaa gaagctacca cgcctggact gtatctgccg acagttgcca acaactttat
    13981 cagcgcaatg gcctgtggtg cgttgggcta caccgacgcc ggtctggtgt ttttaggcgc
    14041 aggcgttttc tcatggctaa gccttgaacc ggtgatcttg cagcgtctgc gtagttcggg
    14101 agaattaccc acggcactgc ggacatcact cggcattcag ctcgctcctg cgctggtggc
    14161 ttgtagtgcc tggctgagcg tcaacggcgg cgagggtgac acgctggcga aaatgctttt
    14221 cggttatgga ctgctgcaac tgctgtttat gctacgtctg atgccatggt atctctccca
    14281 gccatttaat gcttcattct ggagtttctc gttcggcgta tctgcactgg caaccaccgg
    14341 tttgcatctg gggagtggca gcgataatgg atttttccat acgctggcgg tgccgctgtt
    14401 tatctttacc aattttatta ttgcaatact gctcatccgt acttttgcgc ttctgatgca
    14461 gggaaaattg ttagtcagaa ccgagcgcgc cgttttaatg aaagcagagg acaaagaatg
    14521 atcattcgtg acgaaaacta ttttactgat aaatatgaat taacccgcac acactctgaa
    14581 gtactggaag cggtgaaagt ggttaaaccg ggtaaaacgc tggatctggg ctgtggcaat
    14641 ggtcgtaaca gtctttacct ggcagccaat ggttatgatg ttgacgcatg ggataaaaat
    14701 gccatgagta tcgccaacgt cgagcgcatt aaatctattg aaaatctgga taatttacac
    14761 acccgggtcg ttgatctgaa taacctcaca tttgctggac agtacgattt tattctttcg
    14821 actgtggtgc tgatgttcct tgaggctaaa accatcccag ggctgattgc caatatgcaa
    14881 cgttgcacta aacctggcgg ttacaacctg attgtggcgg cgatggatac cgctgattat
    14941 ccatgtaccg tcggcttccc gtttgccttc aaagaaggag aattacgtcg atattacgaa
    15001 ggctgggaga tggtgaaata caatgaagac gtcggcgagc tgcaccgcac cgacgccaac
    15061 ggtaatcgta ttaaactgcg tttcgccacg atgctggcac gtaaaaaatg acccggtaag
    15121 aacaaagcgc gtgaaaattc cccacgctga gatggtttac tgtccttctt ttcggtaagc
    15181 atatttttta tcgaagggat gtgaaattaa tcacagtagt cgaagttttt tagcagctta
    15241 acttactgaa atttaactac tgatgattga cttagcccct tttgcggcat tgactatgtc
    15301 gtctgaaaag gggctaaaaa aatttatttt caccaacact ttttttgcc
//
LOCUS       DAZKFU020000108        15184 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZKFU020000108 DAZKFU020000000
VERSION     DAZKFU020000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.109"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            92..658
                     /gene="yceJ"
                     /locus_tag="ACHOQQ_RS13390"
                     /old_locus_tag="pgaptmp_002678"
     CDS             92..658
                     /gene="yceJ"
                     /locus_tag="ACHOQQ_RS13390"
                     /old_locus_tag="pgaptmp_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4470907.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            662..1237
                     /gene="yceI"
                     /locus_tag="ACHOQQ_RS13395"
                     /old_locus_tag="pgaptmp_002679"
     CDS             662..1237
                     /gene="yceI"
                     /locus_tag="ACHOQQ_RS13395"
                     /old_locus_tag="pgaptmp_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGU4470908.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1279..2331)
                     /gene="trhO"
                     /locus_tag="ACHOQQ_RS13400"
                     /old_locus_tag="pgaptmp_002680"
     CDS             complement(1279..2331)
                     /gene="trhO"
                     /locus_tag="ACHOQQ_RS13400"
                     /old_locus_tag="pgaptmp_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGU4470909.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPACAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2556..3476
                     /gene="lpxL"
                     /locus_tag="ACHOQQ_RS13405"
                     /old_locus_tag="pgaptmp_002681"
     CDS             2556..3476
                     /gene="lpxL"
                     /locus_tag="ACHOQQ_RS13405"
                     /old_locus_tag="pgaptmp_002681"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGU4470910.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3648..4874
                     /gene="mdtG"
                     /locus_tag="ACHOQQ_RS13410"
                     /old_locus_tag="pgaptmp_002682"
     CDS             3648..4874
                     /gene="mdtG"
                     /locus_tag="ACHOQQ_RS13410"
                     /old_locus_tag="pgaptmp_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HGU4470911.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4957..5331
                     /gene="msyB"
                     /locus_tag="ACHOQQ_RS13415"
                     /old_locus_tag="pgaptmp_002683"
     CDS             4957..5331
                     /gene="msyB"
                     /locus_tag="ACHOQQ_RS13415"
                     /old_locus_tag="pgaptmp_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4470912.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5332..5559)
                     /gene="yceK"
                     /locus_tag="ACHOQQ_RS13420"
                     /old_locus_tag="pgaptmp_002684"
     CDS             complement(5332..5559)
                     /gene="yceK"
                     /locus_tag="ACHOQQ_RS13420"
                     /old_locus_tag="pgaptmp_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4470913.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5732..8275)
                     /gene="mdoH"
                     /locus_tag="ACHOQQ_RS13425"
                     /old_locus_tag="pgaptmp_002685"
     CDS             complement(5732..8275)
                     /gene="mdoH"
                     /locus_tag="ACHOQQ_RS13425"
                     /old_locus_tag="pgaptmp_002685"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGU4470914.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPLDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8268..9803)
                     /gene="mdoG"
                     /locus_tag="ACHOQQ_RS13430"
                     /old_locus_tag="pgaptmp_002686"
     CDS             complement(8268..9803)
                     /gene="mdoG"
                     /locus_tag="ACHOQQ_RS13430"
                     /old_locus_tag="pgaptmp_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGU4470915.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPSNSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10196..11353
                     /gene="mdoC"
                     /locus_tag="ACHOQQ_RS13435"
                     /old_locus_tag="pgaptmp_002687"
     CDS             10196..11353
                     /gene="mdoC"
                     /locus_tag="ACHOQQ_RS13435"
                     /old_locus_tag="pgaptmp_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGU4470916.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSLIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11361..12842)
                     /gene="clsC"
                     /locus_tag="ACHOQQ_RS13440"
                     /old_locus_tag="pgaptmp_002688"
     CDS             complement(11361..12842)
                     /gene="clsC"
                     /locus_tag="ACHOQQ_RS13440"
                     /old_locus_tag="pgaptmp_002688"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGU4470917.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12784..13317)
                     /gene="ymdB"
                     /locus_tag="ACHOQQ_RS13445"
                     /old_locus_tag="pgaptmp_002689"
     CDS             complement(12784..13317)
                     /gene="ymdB"
                     /locus_tag="ACHOQQ_RS13445"
                     /old_locus_tag="pgaptmp_002689"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGU4470918.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENSHLYERLLTQQGDE"
     gene            complement(13412..13723)
                     /gene="ymdA"
                     /locus_tag="ACHOQQ_RS13450"
                     /old_locus_tag="pgaptmp_002690"
     CDS             complement(13412..13723)
                     /gene="ymdA"
                     /locus_tag="ACHOQQ_RS13450"
                     /old_locus_tag="pgaptmp_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGU4470919.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNSQVASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13844..14176)
                     /gene="csgC"
                     /locus_tag="ACHOQQ_RS13455"
                     /old_locus_tag="pgaptmp_002691"
     CDS             complement(13844..14176)
                     /gene="csgC"
                     /locus_tag="ACHOQQ_RS13455"
                     /old_locus_tag="pgaptmp_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGU4470920.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14235..14690)
                     /gene="csgA"
                     /locus_tag="ACHOQQ_RS13460"
                     /old_locus_tag="pgaptmp_002692"
     CDS             complement(14235..14690)
                     /gene="csgA"
                     /locus_tag="ACHOQQ_RS13460"
                     /old_locus_tag="pgaptmp_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGU4470921.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14731..>15184)
                     /gene="csgB"
                     /locus_tag="ACHOQQ_RS13465"
                     /old_locus_tag="pgaptmp_002693"
     CDS             complement(14731..>15184)
                     /gene="csgB"
                     /locus_tag="ACHOQQ_RS13465"
                     /old_locus_tag="pgaptmp_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGU4470922.1"
                     /translation="KNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFNQ
                     AAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDAS
                     ISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3768 a         3903 c         3632 g         3881 t
ORIGIN      
        1 ataaatatca ttcaatttgt tatttttgtg ttgcaattca ttctccgaca cgccattttc
       61 tttctctcgc aaattcgttt tggatgcact tatgtcattc acaaataccc ctgaacgcta
      121 tggtgttata tctgcggcct tccattggct aagcgcaatt attgtctacg gcatgtttgc
      181 tttaggtctg tggatggtca cgctcagtta ttacgatggc tggtatcaca aagcacccga
      241 actgcataaa agcatcggta ttttgttaat gatggggctg gttattcgcg ttctgtggcg
      301 cgtcatatct ccaccgccgg gtccgctgcc gagttattcg cccatgactc gtcttgccgc
      361 aaaagctgga catctcgcat tgtacctatt gctgttcgcc atcggcatca gcggctatct
      421 gatctcaact gccgatggta aaccgatcag cgtttttggc tggtttgacg tccccgcgac
      481 ccttgccgac gctggcgcac aggcagactt tgccggtgcc ctgcattttt ggctcgcgtg
      541 gagcgtcgta gtattgtccg ttatgcacgg atttatggcc ctgaagcatc atttcatcga
      601 taaagacgac actctgaagc gcatgctggg aaagtcgtca tctgactatg gagtataaaa
      661 aatgaaaaaa agcctgcttg gtttaacctt cgcgtccctg atgttctctg ccggttcagc
      721 ggttgccgcc gattacaaaa ttgacaaaga aggtcagcac gcctttgtta acttccgcat
      781 ccagcatctt ggctatagct ggttatacgg cacctttaaa gatttcgacg gtacttttac
      841 ctttgatgaa aaaaatccgg ctgccgataa agtgaatgtg acaattaaca ccaccagcgt
      901 cgatactaac cacgccgaac gcgataaaca tcttcgcagt gcggattttc tcaataccgc
      961 aaaatatcca caggcaacat tcacctccac cagcgtgaag aaagacggtg acgaactgga
     1021 tattaccggc gatctgacgc tgaatggtgt aaccaaaccc gtcacgctgg aagcgaaatt
     1081 aattggtcag ggcgacgacc catggggtgg taaacgtgca ggcttcgagg ccgaaggcaa
     1141 aattaagctc aaggacttca atatcaagac agatttaggt ccagcttctc aggaagtgga
     1201 tctgattatt tcagtggaag gcgtacagca gaagtaagag tgaaatgttg ccggatggca
     1261 cgcatccggc aatgatattt attctgttgg atcaggaatg cccagtgttg tattcagacg
     1321 tccacgagac ttattaaaga tcttattgcc attttcacgt cctgcccgac ggcgtcgctg
     1381 ttcttctggc ggtaacgcgc tttcttcgca gcaaatctca ctacaacaac ctttgtattt
     1441 ttccgcacat gctggacact gaataaacag caggtggcag ccatcatttt tacagttggt
     1501 atggctgtcg cacggcgcac cgcactggtg gcaatgcgcg ataatctcat ccgatatacg
     1561 ttcgcccatc cgctcgtcaa aaacaaaatt tttgccaata aaacgcaccg gcaagccctg
     1621 ctcgcgcgcc ttacgggcgt attcaataat tccgccctcg atatgccaga ctttgttgaa
     1681 tccgttatgt ttcatccagg cactggcctt ttcacaacga atgccgccgg tgcagtacat
     1741 gacgattttt ttatctttat gtgcctgcat catctcgact gcttttggca gctgctcacg
     1801 gaaggtatct gccggaattt cgagcgcgtt ttcaaagtgc cccacttcat actcatagtg
     1861 gttacgcatg tcgataaata gtgcatcggg atcgtcaagc atggcgttca cttccgccgc
     1921 ttgcagatac tcaccaacat tgctggcatc aaagtgagga tcgtcaatac cgtcggcaac
     1981 gatacgatcg cggaccttca tgcgcagtac ccagaaagat ttcccatcat catccaacgc
     2041 gatattcagg cgtaaaccct ctaaagccgg gtcgaaggca tagagctgcg cgcgaaatgt
     2101 ttcaacattg ctcgctggta cgctgatttg cgcgttaatg ccctcatgcg ccagatacac
     2161 tcgcccaaaa acattcagcg cggtaaacag ctgatataaa gcgtcacggg tcgccttagg
     2221 atcggcgatg tggaaatact tgtaaaacga aatggtggtt cgcggttcgc tctcagccaa
     2281 cattttggct tttaacgcgt cgttggaaat gcggttgtgt aacactggca tggtgtacgg
     2341 ttcctgcgag atgggaaagt aaaaatccgc ggcatgatat agcaattatc gataattaac
     2401 atccacacat tttacgctac atttgcgcat taaaaattat ttgttattta caagcgcggc
     2461 aatttcgccc agtcttcagc cacaaatttt ggttgcgggc gaaaaaatgc gacaatacat
     2521 acaattgccc gaataggttg aaaaacagga ttgatatgac gaatctaccc aagttctcca
     2581 ccgcactgct tcatccgcgt tattggttaa cctggttggg tattggcgta ctttggttag
     2641 tcgtgcaatt gccctacccg gttatctacc gcctcggttg tggattagga aaactggcgt
     2701 tacgttttat gaaacgacgc gcaaaaattg tgcatcgcaa cctggaactg tgcttcccgg
     2761 aaatgagcga acaagaacgc cgtaaaatgg tggtgaagaa tttcgaatcc gttggcatgg
     2821 gactgatgga aactggcatg gcgtggttct ggccggaccg ccgaatcgcc cgctggacgg
     2881 aagtgatcgg catggaacac attcgtgacg tgcaggcgca aaaacgcggc atcctgttag
     2941 ttggcatcca ttttctgaca ctggagctgg gtgcgcggca gtttggtatg caggaaccgg
     3001 ggattggcgt ttaccgcccg aacgataatc cactgattga ctggctacaa acctggggcc
     3061 gtttgcgctc aaataaatcg atgctcgacc gcaaagattt aaaaggcatg attaaagccc
     3121 tgaaaaaagg cgaagtggtc tggtacgcac cggatcatga ttacggcccg cgctcaagcg
     3181 ttttcgtccc tttatttgcc gttgagcagg ctgcgaccac gaccggaacc tggatgctgg
     3241 cacggatgtc cggcgcatgt ctggtgccct tcgttccacg ccgtaagcca gatggcaaag
     3301 ggtatcaatt gattatgctg ccgccagagt gttctccgcc gctggatgat gccgaaacta
     3361 ccgccgcgtg gatgaacaaa gtggtcgaaa aatgcatcat gatggcacca gagcagtata
     3421 tgtggttaca ccgtcgcttt aaaacacgcc cggaaggcgt tccttcacgc tattaaatct
     3481 cccatgccgg atgcttcaga atggcatccg gcattaccac agcaaatccc cctgatttag
     3541 cgataaaagc tctctggatt gcgccccctg gaagtcgggc gcataattag tgtgcttatc
     3601 ttttcttctt atgttcaccg cgcctggcgc accaacagcg gattgctatg tcaccctgtg
     3661 aaaatgacac ccctataaac tggaaacgaa acctgatcgt cgcctggcta ggctgttttc
     3721 ttaccggtgc cgccttcagt ctggtaatgc ccttcttacc cctctacgtt gagcagcttg
     3781 gcgttaccgg ccactccgcc ctgaatatgt ggtccggtat tgtcttcagc attacatttt
     3841 tattttcggc catcgcctca ccgttttggg gtggactcgc cgaccgtaaa ggccgaaaac
     3901 tcatgctatt acgctctgct ctcggcatgg gcatcgtgat ggtgttgatg gggctggcac
     3961 aaaatatctg gcagtttttg atccttcggg cgcttcttgg gttacttggc ggatttgtcc
     4021 ccaacgctaa tgctcttatc gccacacaag taccgcgtaa taaaagcggc tgggcactgg
     4081 gtacgctctc cacaggcggc gttagcggtg cgttgctcgg cccaatggct ggcggcctgc
     4141 tcgccgatag ctacggctta cgtccggtat tctttattac cgccagtgtg ctcatactct
     4201 gctttttcgt caccctgttc tgcatcagag aaaaattcca gccggtcagc aaaaaagaga
     4261 tgctgcacat gcgggaagtg gtgacatcac ttaaaaaccc gaaactggta ctcagcctgt
     4321 ttgtcactac gttaatcatc caagtggcga cgggctcaat tgcccccatt ctgacgctgt
     4381 atgtccgcga actggcgggt aacgtcagta acgtcgcctt tatcagtggc atgatcgcct
     4441 cggtgccagg cgtggcggct ctgctgagtg caccacgact cggcaaactt ggcgatcgaa
     4501 tcggccccga aaagatcctg attacggcgc tgatcttctc tgtactgctg ttgatcccaa
     4561 tgtcatacgt tcagacgcca ttacaacttg ggattttacg ttttttgctc ggtgccgccg
     4621 atggtgcact actccccgcc gtacagacac tgttggttta caactcgagc aaccaaatcg
     4681 ccggacgtat cttcagctat aaccaatcgt ttcgtgatat tggcaacgtt accggaccat
     4741 tgatgggagc agcgatttca gcgaactacg gtttcagagc ggtatttctc gtcaccgctg
     4801 gcgtagtgtt attcaacgca gtctattcat ggaacagtct acgtcgtcgt cgaatacccc
     4861 aggtatcgaa ctgatttttc gcctttcata cttgcaaaag cggagaatca gctatccttt
     4921 tccctgaaac ctcatcaact caaagggaga atcgtgatga ccatgtacgc aacgcttgaa
     4981 gaagccattg acgctgcacg cgaagaattt cttgcagaca accccggcat cgacgccgaa
     5041 gacgcgaatg tgcaacagtt caatgcccaa aaatacgttt tgcaggacgg cgacatcatg
     5101 tggcaagttg agttttttgc cgacgaaggg gaagaaggtg aatgtttacc tatgcttagc
     5161 ggtgaagccg cgcaaagtgt ttttgatggc gactatgatg agatagagat ccgccaggag
     5221 tggcaggaag agaatacatt acatgaatgg gacgaggggg aatttcagct tgagccaccg
     5281 ctggataccg aggaaggacg cgcagcagct gatgagtggg atgaacgtta atcactcata
     5341 cgggccatga tgaatgtcga tcggcagcag taaagtatca aaaaccaggg agaatggcag
     5401 atcaaggatt gtgacataac gccaggcgga gtcacgcaca tcccattgca caccgggata
     5461 atattggttg ccatgcccct gccccggtat agtgcggcta atgatgctgc cacagccact
     5521 cagcaaggtc accatgatgc tcaccacaat taaacgcatt tgatacctct tccgtaaaaa
     5581 aataccggca taacgccggt atttttattg gctcgttgtt tttgccagat gcgacgtgaa
     5641 cgccttacct aacctacaaa atcttgcaaa atcaataaat tgcaggaacg atgtaggcct
     5701 gataagcgta gcgcatcagg caactacgtt tttattgcga agccgcatcc ggtttacgca
     5761 atgccagtgg attgagcttt atcccttcgt aataactcac ccatgaagaa tatctctcag
     5821 gggaattcca gacgcggaaa tgcagacggg ccatcgtcac cggatcgctt agcagcacaa
     5881 ggcgacgatc gcgattcagc ttctctggcg tctcgttcag cgcctgttca acgtggcggt
     5941 cacgggcgat ttccagcacc ttactggcgc ggtgacgcgc ggtcgccatt gcggtggcca
     6001 gagcgttaaa tgacgggtta aacactgcgt gcataaaacc atcatcaagg gagcgttgac
     6061 ggttcatctc aaggaaccga tcggtatcaa ccagtacctg cggcggtgaa tactcttccg
     6121 ggatcaggaa cagtttccag cgtttggtgc gcagaccaac ggtggcacgg ctggaaatca
     6181 ccgaaacaaa cggtgacagg atcaacgaga agacaatcgg tgccagccag aacaggaaac
     6241 gcagatccaa ccacgccatc ccaacagccc acactaaccc taacagcagc tgtgagccgt
     6301 ggcgtttgaa cgcttcaccc caggaagtgg agtcatcatc acgctgcggt gaattccaca
     6361 ccacttccca gccaaggaac gcgctgacga cgaagaccgt atggaacagc atgcgtaccg
     6421 gagccagcag cacggaaaac agcacttcca gcagcaacga taatgtaacg cgccagaagc
     6481 cgccatattc tttcgttcct ttgcaccaaa taagcaaaat gctcaatagc tttggcagga
     6541 acaacagcac catggtcgaa gcaaaaagtg caatcgccag ctcaggacgc cactgcggcc
     6601 acaccgggaa caactgccgt ggttgcagga agtattgcgg ttcggttaac gcatgcacta
     6661 cctgcaatgc agtagagagc gcgaggaaca taaaccacag cggagcggag agataagaca
     6721 tcacgcccgt caggaacacc gcacggtgaa ccgggtgcat acccttaacc aggaacagac
     6781 ggaagttcat caggttaccg tggcaccagc ggcggtcacg ttttagctca tcaagtaagt
     6841 taggcggtaa ttcttcataa gaacctggga gatcgtaagc aatccagact ccccaacctg
     6901 cacggcgcat caacgccgct tccacgaagt catgtgacag gattgaaccg gcaaaggaac
     6961 cttcgcccgg cagcggagcc agtgcgcagt gctcgataaa cggtttcacg cggataatcg
     7021 cgttatgtcc ccagtagtgc gactcgccaa gttgccagaa gtgcaaaccg gctgtaaaca
     7081 gtggcccata cacgcgggtc gcgaactgct gacagcgcgc atacagcgta tccataccgg
     7141 acgctttcgg cgacgactga atgatcccgg cgttcgggtt ggcttccatc aagcgcacca
     7201 ggccgcataa acaatcaccg gtcattaccg agtcagcatc cagcaccacc atgtagctgt
     7261 actggctgcc ccagcgacgg cagaagtcat cgatattacc gcttttacgc ttcacgcgac
     7321 gacggcggcg gcgatagaaa atctgacctt cgccaccgac ttcagcgata agctccatcc
     7381 aggctttttg ctctgcgacg cagatatccg ggttataact gtcactcaga atgtagacat
     7441 caaagtgttt ggcattcccg gtggctttta ctgattccca cgttgcacgc aggccagcaa
     7501 aaacacggtt cacgtcttcg ttacagatag gcatgatcaa cgccgtgcga tgctccgggt
     7561 ttaatggttc atcgccaact gttgacgcag atatactgta tttatcgcga ccaataagca
     7621 gttgcaggaa gcccattaac gccgtccaga atccggcgga cacccaacag aacagtaccg
     7681 caaagaggat caggataccg gtttgcagca tataaggcag aagctgcata aaggaaaccc
     7741 acaaatcctg accaaccata tccaaaggat taatcagcgc ccaaccctga taaggaagaa
     7801 tggtcttcat ataccaggtc gcgacgacag tttgcgcgag cgtcaggatc aacagaatgt
     7861 aacggcggat ggtaccgacg gtacgccact tttgctcgct ctcctgctct tctttggtca
     7921 aacgagccag atagcgcggc gtgacatcgc gtccacgcag gcgatcccag aaacggccta
     7981 ccgggttggt acgccacggg tcgggaaaca tcgaggagcg ttttgcttct ggcatcgcct
     8041 taagctgatc gcgcccttcg tcgtctttaa ttaactgtcc atcagcaagt gaatctggcc
     8101 aggcttgttc cagacgcgcc tttaccgagc cttgcgggga gtcatcctcc cgcgcccagg
     8161 tgcggtgttc ggcatccagc gcctgatgaa cggcgcggat atcagtcttc ggcaatgccg
     8221 ctttctcgct tgcggcgatg ggcattgcgt caatgtactc agttgtctta ttcattggca
     8281 ggtaactggt agctccaggt ttcactcaac gtctgatcgg cattcaccag cgcagctcgc
     8341 atttcagtgg ttttcttggc atctttcact ttcacacgca tcaccagacg ccagcctttg
     8401 gtaaccgggt tatagcgcac cgtgctttca actatctcac cattatcacc aatgctggtt
     8461 tgcgctgtga ccggggtatc ctctggcagt tttttcatct ctgcgccggt aaaatcgacc
     8521 acaaaggcga tagtaccgtc aggctggcgg atcaggttcg actgcttcac atcccccgtt
     8581 gaacgacgcg tttgttgcac ccatgcgtta tctggtgcat gcagtttgtc ttcatcacgg
     8641 ctgaaggtga tggtgtattt aaagttcatc tctttacccg gctccggcag ctgatccggc
     8701 gtccagtaag cgacgatgtt atcgttagtt tcatcgttgg ttggaatttc caccagctca
     8761 acgctgcctt tgccccactc ccctttcgga gtcacccatg cgcttggacg gagatcgtaa
     8821 cgatcatcga gatcttcaaa gcgggagaaa tcacgaccgc gctgcaacag accgaagcct
     8881 tgcgggtttt ccatggagaa gctgctgacc gctaaatgtt tcgggttatt caacggacgc
     8941 cagatccatt cgccattacc ggcatggata gagagaccgt tagagtcgtg caactccgga
     9001 cgatagttat ttgcaggcga cggttggttc ggcccaaaca ggaacatact ggttaacggt
     9061 gcaaccccca gtttgccgac tttatcgcgc agatagattt tcgactgcac atccacaacc
     9121 gtgtcacgcc ctggcataac tacgaatttg taagcacctg tcgcgcgcgg cgagtcaagc
     9181 aatgcataaa tggttaaacg tttatcagtt ggttttggac gctcgatcca gaactctttg
     9241 aagcgtggaa attcttcacc cgatggcaag gcggtatcaa ttgccagacc gcgggcagaa
     9301 aggccataaa cctgacctgc accaatcacg cggaaatagc tggccccgag catgctgacg
     9361 atttcatcgt ttttatcttt gctgttgctc gggtaaagca ctttaaaacc ggcaaaacca
     9421 aggtctttta ccgtgtcttt gtcatgctga acatcgccga aagtgaaata atccgggctg
     9481 tatttgattc gtttgactgc ggtggcagtc acttcattta ttttgaccgg ggtatcgaag
     9541 tacatcccct gatggtagaa ctcgagtttg aatggggtct tcagattgtt ccagtacgct
     9601 ttgtcatgat taaactggat ctgctgatag tccgcgtatt tcatatcgcg gaaaacggag
     9661 ggcaagttgc ttttgggcgc ctcatagcct ttcccggcta aggattgagc ttgctttgcg
     9721 acatcatcaa tactgaaagc ccagcttgaa gatgtataca gggttaacat tactgcagca
     9781 ctcaaccaac gcattttcat catttgtagt ttatgtttca taattagtaa gcacttcccc
     9841 ctttgtgtgc ttatatcgat ccgatctatt ttaatggata accagccatt ccgacaacag
     9901 aatcccggta tcgttcagca cgctggctca tggattaagc acctgattgc aaccgttttc
     9961 actttccgtg cttctcctaa agacagaagc atcgacttcg tgtagggttt gccagcaaat
    10021 ttatttatca accgtcttac agacgcttcc attctggtta gtggtagcga tgatgctatg
    10081 cataaaaact tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc
    10141 taaaggcata acccgacagg gataagacga aaaatccgag attagttaac catatatgaa
    10201 tccagtaccc gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt
    10261 agggatccct tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc
    10321 cgaaccgtca ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt
    10381 atttttcgtt atatccggct acttttccta catgcttttt ttacgctatc ccttgaaaaa
    10441 atggtggaaa gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact
    10501 gacattgccg caatttatta tgctgcaata cgtcaaaggg aaagcggaaa gttggcctgg
    10561 gctgtcattg tatgacaaat ataatacgtt agcctgggaa ttaatatcgc acctgtggtt
    10621 tttactggtg ttagtggtca tgacgacgct gtgcgtatgg atatttaaac gcatcagaaa
    10681 taatttagaa aattctgata aaacgaataa aaaattctcg atggtaaaac tatcgctgat
    10741 ttttttgtgc ctcggcatcg gttatgcggt aataagaaga acgattttta ttgtgtatcc
    10801 gcccattctg agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttgcc
    10861 gttctttatc ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac
    10921 gccgtctcgt ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca
    10981 gcgctatggc agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct
    11041 cggtctgtgg atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc
    11101 agcgcgggtg acttactttg ttaacgcatc gctatttatc tatctggttc accacccgtt
    11161 aacgctgttt ttcggcgcgt acattacacc gcacatcacc tccaactggc ttggttttct
    11221 ctgtggcctg atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat
    11281 cccattactg aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc
    11341 accagcccgt taagccgcgt ttacaataac cattccacgg gcaatatcga cgccagtctg
    11401 accataaccc gcttccagaa actggtggcg ggttcttttt tgagaacaat ctctttcttt
    11461 gcatgacgat caacccagtt gatccgcccc cacctgtcca gacggagctg ccaggccgca
    11521 tcatactggc tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc
    11581 acgaagccca tttcagtatt gagcaatgtc gaacgtggat cgaaattgaa agagccgata
    11641 aacaccgttt taccatcgat gctaaaggtt ttagcatgca ggctggctcc ggaattaccg
    11701 gttatgccgc gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat
    11761 tccacgccat agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca
    11821 acatcgttag cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc
    11881 cgtaagagtt gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg
    11941 cgttcactgg gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt
    12001 gccttgccct cccctttcgc cggatcatca cttaataaac gtgttttcgc ccagataagc
    12061 ggcaacgttc catcaaccag atgatttata aacggactgg attccatttt gcgtaagtaa
    12121 cgatgcgtca tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca
    12181 ccttccggga catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg
    12241 gcgaaatcat cggcaacgtc ctctactacg ggtcctattg ccatgacatc taaatccgaa
    12301 aaaagtggct cttcccctgc cccaaaatag gcatcaccaa tatttcgccc ccctaccagc
    12361 gtcaccacgc catcgacagt gaaactttta ttgtgcatac ggcgattgag acgggaaaag
    12421 tcggtgatat aaccaagcgg acgcagcaag cgaaacgaga aaggattaaa aagccggact
    12481 tcaatgcggg gatggctgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg
    12541 tcgtccagca acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac
    12601 agtaaccgac ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct
    12661 gccatttcgg ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa
    12721 agaccacact gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc
    12781 aaatcattca tctccttgtt gggtaaggag tctttcgtag aggtgggagt tttcttcatc
    12841 ataacagaca aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt
    12901 ttttactgcg atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc
    12961 aggaaaagcc actgacgtat agctgtttgc cgccaccagt cgcaggctat tgagataggc
    13021 atcctgcaaa agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg
    13081 cactacggct ttagcaggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca
    13141 atcgccctgc tgttgcctaa cttttaaaca ggcatccagc agggccggac ccgctgcgcg
    13201 atgaatggcc ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat
    13261 cacatcaacg gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac
    13321 gcctccttac accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt
    13381 ttctgctcag caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca
    13441 gctttttctg tttatcaaga tactgcactt ttactgaggc tacttgcgag tttttaacat
    13501 tgccgctcat taacgctttg ctggaatagg tttttcccgg aacagaacca ttctgtgggc
    13561 agttcatttc aacgggtgat gactgggtgc tgacgtcaca tgccggttct acaatttggc
    13621 cataaaaatg gatcacgccc ccttgcgcgg tgcttcctgc gatggcaata aaaggaaaaa
    13681 acaaactgcc gagcataaga gagtttaaaa aaggtctgaa catttattct atttcctcaa
    13741 tgattagtca tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag
    13801 accattctct ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag
    13861 agggcggcca ttgttgtgat aaatgaagtg actgtccatc agaaacagta acaactattt
    13921 tcacccgatc gtccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag
    13981 caggcaatga aagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca
    14041 gggacaatat ttgtactctg cacagacaag attgagtaag agtgacttca ggaataatgg
    14101 tatacacatc cccttgctgg gtcgtattaa aggttatctg actggaaagt gccgcaagga
    14161 gtaataacgc attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta
    14221 atacaaatga tgtattagta ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc
    14281 acgttgacgg aggagttaga tgcagtctgg tcaactgcag caccgttgcc accaccgaac
    14341 tgtttaaccg tcatttcaga atttttgccg ttccactgat caagagtagc gctgttaccg
    14401 aagccacgtt gggtcagatc gattgagctg tcatccgagc cctgaccaac atctgcacca
    14461 ttaccgccgc catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca
    14521 agtgcagagt taccgccacc gtactggtaa atgttcagct cagaatttgg gccgctatta
    14581 ttaccgccac caccgtggtt accgccgccg ccgtactgag gaacaacacc tgccagagcg
    14641 ctaccggaga atacgattgc tgcaattgct gctactttta aaagtttcat gtaaaacccc
    14701 catcggattg atttaaaagt cgaatggaaa ttaacgttgt gtcacgcgaa tagccatttg
    14761 cgactgtctc tgcactacaa ttgccgtttt ttgagtacca tactgtgtaa tatttgcttt
    14821 attaccagaa cctttctgga taatcatcgc agtattacca taagcacctt gcgaaatact
    14881 ggcatcgttg gcactgcccg cctgatcaat atatgcaagg ttataatctc ctgtctggtc
    14941 aatctttgcc cggttgctac taccttcttg cgcaacaacc gccaaaagtt ttgagcctcc
    15001 ctgccgtaac tgagcactat tattagtccc agcttgacca attatggctg cctgattaaa
    15061 tgaagactta ctcaattcat ttaccgcgaa gttatattct gaattagcta aatcataacc
    15121 tgctgcggct gcaatcccag gcgcacccag tattgttaac atcataaata acaatttgtt
    15181 tttc
//
LOCUS       DAZKFU020000109        15101 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZKFU020000109 DAZKFU020000000
VERSION     DAZKFU020000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.110"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..626
                     /locus_tag="ACHOQQ_RS13470"
                     /old_locus_tag="pgaptmp_002694"
     CDS             81..626
                     /locus_tag="ACHOQQ_RS13470"
                     /old_locus_tag="pgaptmp_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU4470923.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            601..2526
                     /locus_tag="ACHOQQ_RS13475"
                     /old_locus_tag="pgaptmp_002695"
     CDS             601..2526
                     /locus_tag="ACHOQQ_RS13475"
                     /old_locus_tag="pgaptmp_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4470924.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2523..2729
                     /locus_tag="ACHOQQ_RS13480"
                     /old_locus_tag="pgaptmp_002696"
     CDS             2523..2729
                     /locus_tag="ACHOQQ_RS13480"
                     /old_locus_tag="pgaptmp_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HGU4470925.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2726..4327
                     /locus_tag="ACHOQQ_RS13485"
                     /old_locus_tag="pgaptmp_002697"
     CDS             2726..4327
                     /locus_tag="ACHOQQ_RS13485"
                     /old_locus_tag="pgaptmp_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4470926.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4308..5627
                     /locus_tag="ACHOQQ_RS13490"
                     /old_locus_tag="pgaptmp_002698"
     CDS             4308..5627
                     /locus_tag="ACHOQQ_RS13490"
                     /old_locus_tag="pgaptmp_002698"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4470927.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLAAGEAPATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGIMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATALQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5637..5969
                     /locus_tag="ACHOQQ_RS13495"
                     /old_locus_tag="pgaptmp_002699"
     CDS             5637..5969
                     /locus_tag="ACHOQQ_RS13495"
                     /old_locus_tag="pgaptmp_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4470928.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6024..7049
                     /locus_tag="ACHOQQ_RS13500"
                     /old_locus_tag="pgaptmp_002700"
     CDS             6024..7049
                     /locus_tag="ACHOQQ_RS13500"
                     /old_locus_tag="pgaptmp_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4470929.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     THARGLRTYGCIQDADALSEGINASPRYPKNWKTSGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7091..7486
                     /locus_tag="ACHOQQ_RS13505"
                     /old_locus_tag="pgaptmp_002701"
     CDS             7091..7486
                     /locus_tag="ACHOQQ_RS13505"
                     /old_locus_tag="pgaptmp_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4470930.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            7498..7851
                     /locus_tag="ACHOQQ_RS13510"
                     /old_locus_tag="pgaptmp_002702"
     CDS             7498..7851
                     /locus_tag="ACHOQQ_RS13510"
                     /old_locus_tag="pgaptmp_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4470931.1"
                     /translation="MADFDNQFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7863..8441
                     /locus_tag="ACHOQQ_RS13515"
                     /old_locus_tag="pgaptmp_002703"
     CDS             7863..8441
                     /locus_tag="ACHOQQ_RS13515"
                     /old_locus_tag="pgaptmp_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4470932.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8438..8833
                     /locus_tag="ACHOQQ_RS13520"
                     /old_locus_tag="pgaptmp_002704"
     CDS             8438..8833
                     /locus_tag="ACHOQQ_RS13520"
                     /old_locus_tag="pgaptmp_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4470933.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8841..9581
                     /locus_tag="ACHOQQ_RS13525"
                     /old_locus_tag="pgaptmp_002705"
     CDS             8841..9581
                     /locus_tag="ACHOQQ_RS13525"
                     /old_locus_tag="pgaptmp_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4470934.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            9597..10019
                     /locus_tag="ACHOQQ_RS13530"
                     /old_locus_tag="pgaptmp_002706"
     CDS             9597..10019
                     /locus_tag="ACHOQQ_RS13530"
                     /old_locus_tag="pgaptmp_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4470935.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            10001..10435
                     /locus_tag="ACHOQQ_RS13535"
                     /old_locus_tag="pgaptmp_002707"
     CDS             10001..10435
                     /locus_tag="ACHOQQ_RS13535"
                     /old_locus_tag="pgaptmp_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4470936.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10428..12989
                     /locus_tag="ACHOQQ_RS13540"
                     /old_locus_tag="pgaptmp_002708"
     CDS             10428..12989
                     /locus_tag="ACHOQQ_RS13540"
                     /old_locus_tag="pgaptmp_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4470937.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSVSGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAETAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHADLLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMTGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            12986..13315
                     /locus_tag="ACHOQQ_RS13545"
                     /old_locus_tag="pgaptmp_002709"
     CDS             12986..13315
                     /locus_tag="ACHOQQ_RS13545"
                     /old_locus_tag="pgaptmp_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4470938.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13315..14013
                     /locus_tag="ACHOQQ_RS13550"
                     /old_locus_tag="pgaptmp_002710"
     CDS             13315..14013
                     /locus_tag="ACHOQQ_RS13550"
                     /old_locus_tag="pgaptmp_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4470939.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14018..14761
                     /locus_tag="ACHOQQ_RS13555"
                     /old_locus_tag="pgaptmp_002711"
     CDS             14018..14761
                     /locus_tag="ACHOQQ_RS13555"
                     /old_locus_tag="pgaptmp_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4470940.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAKMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGVIHK
                     FRCVPHLTGRRFEHGATDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNMEAT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            14758..>15101
                     /locus_tag="ACHOQQ_RS13560"
                     /old_locus_tag="pgaptmp_002712"
     CDS             14758..>15101
                     /locus_tag="ACHOQQ_RS13560"
                     /old_locus_tag="pgaptmp_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4470941.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLCDGWYQVRIAGRDVSTSGLTVQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVL"
BASE COUNT         3404 a         3863 c         4769 g         3065 t
ORIGIN      
        1 gaaaggaaac gacaggtgct gaaaacgagc tttttggcct ctgtcgtttc ctttctctgt
       61 ttttgtccgt ggaatgaaca atggaagtca acaaaaagca gctggctgac attttcggcg
      121 cgagtatccg taccattcag aactggcagg agcagggaat gcccgttctg cggggtggtg
      181 gcaagggtaa tgaagtgctt tatgactctg ccgccgccat aaaatggtat gccgaaaggg
      241 atgctgaaat tgagaacgaa aagctgcgcc gggaagttga agaactgcgg caggccagcg
      301 agacagatct ccagccaggg actattgagt acgaacgcca tcgacttacg cgtgcgcagg
      361 ccgacgcaca ggagctgaaa aatgccagag actccgctga agtggtggaa accgcattct
      421 gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggatccccc
      481 tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg
      541 atatcatcaa agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga
      601 gtgaatatat cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc
      661 tcactgttca ggccggagcc acagaccgcc gttgaatggg cggatgccaa ttactatctc
      721 ccaaaagaat ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc
      781 atgaatgcga tgggcagcga ctacatccgt gaggtgaatg tggtgaagtc tgcccgtgtc
      841 ggttattcca aaatgctgct gggtgtttat gcctacttta tagagcataa gcagcgcaac
      901 acccttatct ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag
      961 ccgaccatcc gcgatattcc gtcgctgctg gcgctggctc cgtggtatgg caaaaagcac
     1021 cgggataaca cgctcactat gaagcgtttt tccaatggtc gtggcttctg gtgcctgggc
     1081 ggtaaagcgg caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta tgatgaactt
     1141 gctgcctttg atgaggatat tgaacaggaa ggctctccga cgttcctggg tgacaagcgt
     1201 attgaaggct cggtctggcc aaagtccatc cgtggctcca cgcccaaagt gagaggcacc
     1261 tgtcagattg agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc
     1321 ccgcattgcg gggaggagca gtatcttaaa tttggcgaca aagagacgcc gtttggcctc
     1381 aaatggacgc cggatgaccc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc
     1441 atccgccagc aggagctgga ctttactgat gcccgttata tctgcgaaaa gaccgggatc
     1501 tggacccgtg atggcattct ctggttttcg tcatccggtg aagagattga gccacctgac
     1561 agtgtgacct ttcacatctg gacagcgtac agcccgttca ccacctgggt gcagattgtc
     1621 aaagactgga tgaaaacgaa aggggatacg ggaaaacgta aaaccttcgt aaacaccacg
     1681 ctcggtgaga cgtgggaggc gaaaattggc gaacgtccgg atgctgaagt gatggcagag
     1741 cggaaagagc attattcagc gcccgttcct gaccgtgtgg cttacctgac cgccggtatc
     1801 gactcccagc tggaccgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc
     1861 tggctgattg accggcagat tattatgggc cgccacgacg atgaacagac gctgctgcgt
     1921 gtggatgagg ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcggtatcc
     1981 cgtatctgct gggatactgg cgggattgac ccgaccattg tgtatgaacg ctcgaaaaaa
     2041 catgggctgt tccgggtgat ccccattaaa ggggcatccg tctacggaaa gccggtggcc
     2101 agcatgccac gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg tacggatacc
     2161 gcgaaagagc agatttataa ccgcttcaca ctgacgccgg aaggggatga accgcttccc
     2221 ggtgccgttc acttccccaa taacccggat atttttgatc tgaccgaagc gcagcagctg
     2281 acggctgaag agcaggtcga aaaatgggtg gatggcagga aaaaaatact gtgggacagc
     2341 aaaaagcgac gcaatgaggc gctcgactgc tttgtttatg cgctggcggc gctgcgcatc
     2401 agtatttccc gctggcagct ggatctcagt gcgctgctgg cgagcctgca ggaagaggat
     2461 ggtgcagcaa ccaacaagaa aacactggca gattacgccc gtgccttatc cggagaggat
     2521 gaatgacgcg acaggaagaa cttgccgctg cccgtgcggc actgcatgac ctgatgacag
     2581 gaaaacgggt ggcaacggta cagaaagacg gacggagagt ggagtttacg gccacttccg
     2641 tgtctgacct gaaaaaatac attgcggagc tggaagtgca gaccggcatg acacagcgac
     2701 gcaggggacc tgcaggattt tatgtatgaa aacgcccacc attcccaccc ttcttgggcc
     2761 ggacggcatg acatcgctgc gcgaatatgc cggttatcac ggtggtggca gcggatttgg
     2821 tgggcagctg cgggcgtgga acccaccggg tgaaagtgtg gatgcagccc tgttgcccaa
     2881 ctttacccgt ggcaatgccc gcgcagacga tctggtacgc aataacggct atgctgccaa
     2941 cgccatccag ctgcatcagg atcatatcgt cgggtccttt ttccggctaa gtcatcgccc
     3001 aagctggcgt tatcttggca tcggggagga agaagcccgt gccttttccc gcgaggttga
     3061 agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg
     3121 cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt
     3181 cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt
     3241 cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg
     3301 tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc
     3361 tggctggatg ccgcagaaat ggacatggat accccgtgaa ttacccggcg ggcgcgcctc
     3421 gttcattcac gtttttgaac ccgtggagga cgggcagacc cgcggtgcaa atgtgtttta
     3481 cagcgtaatg gagcagatga agatgctcga cacgctgcag aacacgcagc tgcagagcgc
     3541 cattgtgaag gcgatgtatg ccgccaccat tgaaagtgag ctggatacgc agtcagcgat
     3601 ggattttatt ctgggcgcga acagtcagga gcagcgggaa aggctgacgg gctggattgg
     3661 tgaaattgcc gcgtattacg ccgcagcacc ggtccgtctg ggaggcgcaa aagtgccgca
     3721 cctgatgccg ggggactcac tgaacctgca gacggctcag gatacggata acggctactc
     3781 cgtgtttgag cagtcactgt tgcggtatat cgctgccggg ctgggtgtct cgtatgagca
     3841 gctttcccgg aattacgccc agatgagcta ctccacggca cgggccagtg cgaacgagtc
     3901 gtgggcgtac tttatggggc ggcgaaaatt cgtcgcatcc cgtcaggcga gccagatgtt
     3961 tctgtgctgg ctggaagagg ccatcgttcg ccgcgtggtg acgttacctt caaaagcgcg
     4021 cttcagcttt caggaagccc gcagtgcctg ggggaactgc gactggatag gctccggtcg
     4081 tatggccatc gatggtctga aagaagttca ggaagcggtg atgctgatag aagccggact
     4141 gagcacctac gagaaagagt gcgcgaaacg cggtgacgac tatcaggaaa tttttgccca
     4201 gcaggtccgt gaaacgatgg agcgccgcgc agccggtctt aaaccgcccg cctgggcggc
     4261 tgcggcattt gaatccgggc tgcgacaatc aacagaggag gagaagagtg acagcagagc
     4321 tgcgtaatct cccgcatatt gccagcatgg cttttaatga gccgctgatg cttgaacccg
     4381 cctatgcgcg ggttttcttt tgtgcgcttg caggccagct tgggatcagc cgcctgacgg
     4441 atgcagtatc cggcgacagc ctggctgccg gagaggcacc cgcgacgctg gcgttatccg
     4501 gtgatgatga cggaccacga caggcccgca gttatcaggt catgaacggc atcgccgtgc
     4561 tgccggtgtc cggcacgctg gtcagccgga cgcgggcgct gcagccgtat tcgggaatga
     4621 ccggttacaa cggcattatc gcccgtctgc aacaggctgc cagcgatccg atggtggacg
     4681 gcattctgct cgatatggac acgccaggcg gaatggtggc gggagcattt gactgcgctg
     4741 acatcatcgc ccgtgtgcgt gacataaagc cggtatgggc gctggccaac gacatgaact
     4801 gcagtgcagg tcaactgctt gccagcgccg cctcccggcg tctggtcacg cagaccgccc
     4861 ggacaggctc catcggcatc atgatggctc acagtaatta cggtgctgcc ctggagaaac
     4921 agggcgtgga aatcacgctg atttacagcg gcagccataa ggtggatggc aacccctaca
     4981 gccatctacc ggatgatgtc cgggaaacac tgcagtcccg gatggatgca acccgccgga
     5041 tgtttgcaca gaaggtgtcg gcatataccg gcctgtccgt gcaggctgtg ctggataccg
     5101 aggctgcagt gtacagcggt caggaggcca ttgatgccgg actggctgat gaacttgtta
     5161 acagcaccga tgcgatcacc gtcatgcgtg atgcactgga tgcacgtaaa tcccgtctct
     5221 caggagggcg aatgaccaaa gagactcaat caacaactgt ttcagccact gctttgcagg
     5281 ctgacgttac tgacgtggtg ccagcgacgg agggtgaaaa cgccagcgcg gcgcagccag
     5341 acgtgaacgc gcagatcacc gctgcggttg cggcagaaaa cagccgcatt atggggatcc
     5401 tcaactgtga ggaggctcac ggacgcgaag aacaggcccg cgtgctggca gaaacccccg
     5461 gtatgaccgt ggaaacggcc cgccgcattc tggccgcagc accacagagt gcacaggcgc
     5521 gcagtgacac tgcgctggat cgtctgatgc agggggcacc ggcaccgctg gctgcaggta
     5581 acccggcatc tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg atgtttatga
     5641 cgagcaaaga aacctttacc cattaccagc cgctgggcaa cagtgacccg gctcataccg
     5701 caaccgcgcc cggcggattg agtgcgaaag cgcctgcaat gaccccgctg atgctggaca
     5761 cctccacccg taagctggtt gcgtgggatg gcaccaccga cggtgctgcc gttggcattc
     5821 tggcggttgc tgctgaccag accagcacca cgctgacgtt ctacaagtcc ggcacgttcc
     5881 gttatgagga tgtgctctgg ccggaggctg ccagcgacga gacgaaaaaa cggaccgcgt
     5941 ttgccggaac ggcaatcagc atcgtttaac cttacccttc atcactaaag gccgcctgtg
     6001 tggctttttt tacgggattt tttatgtcga tgtacacaac cgcccagctg ctggcggcaa
     6061 atgagcagaa atttaagttt gatccgctgt ttctgcgtct ctttttccgt gagagctatc
     6121 ccttcaccac ggagaaagtc tatctctcac aaattccggg actggtaaac atggcgctgt
     6181 acgtttcgcc gattgtttcc ggtgaggtta tccgttcccg tggcggctcc acctctgaat
     6241 ttacgccggg atatgtcaag ccgaagcatg aggtcaatcc gcagatgacc ctgcgtcgcc
     6301 tgccggatga agatccgcag aatctggcgg acccggctta ccgccgccgt cgcatcatca
     6361 tgcagaacat gcgtgacgaa gagctggcca ttgctcaggt cgaagagatg caggccgttt
     6421 ctgccgtgct caagggcaaa tacaccatga ccggtgaagc cttcgatccg gttgaggtgg
     6481 atatgggccg cagtgtggcg aacaacatca cgcagtccgg cggcacggag tggagcaagc
     6541 gtgacaagtc cacgtatgac ccgaccgacg atatcgaagc ctacgcgctg aacgccagcg
     6601 gcgtggtgaa tatcatcgtg tttgacccga aaggctgggc gctgttccgt tccttcaaag
     6661 ccgtcaagga gaagctggat acccgtcgcg gctctaattc cgagctggag acagcggtaa
     6721 aagacctggg caaagcggtg tcctataagg ggatgtatgg cgatgtggcc atcgtcgtgt
     6781 attccggaca gtacgtggaa aacggcgtca aaaagaactt cctgccggac aacacgatgg
     6841 tgctggggaa cactcatgca cgcggtctgc gcacctatgg ctgtattcag gatgcggatg
     6901 cattgagtga gggtattaat gcgtctcccc gttatccgaa aaactggaag acatccggcg
     6961 atccggcgcg agagttcacc atgattcagt cagcaccgct gatgctgctg gctgatcctg
     7021 atgagttcgt gtccgttcaa ctggcgtaat catggccctt cggggccatt gtctctctgt
     7081 ggaggagtcc atgacgaaag atgaactgat tgcccgtctc cggtcgctgg gtgagcaact
     7141 gaaccgtgat gtcagcctga cggggacgaa agaagaactg gcgctccgtg tggcagagct
     7201 ggaagaggag cttgatgaca cggatgacgc agccggtcag gacacgtctg tcagcccgga
     7261 aaatgcgctg accggacatg aaaatgaggt ggtatcagca cagacggata ccgtgactga
     7321 tacggctgct ctggtcacgg ttgtggcact ggtgacgctg cataccgatg cacttcacgc
     7381 cacgcgggat gaacctgtgg catttgtgct gccgggaacg gcgttccgtg tctctgccgg
     7441 tgtggcagcc gaaatgacag aacgtggcct ggccagaatg caataacggg aggcgctgtg
     7501 gctgatttcg ataaccagtt cgatgctgcc attgcccgcg ccgatgaaac gatacgcggg
     7561 tacatgggaa cgtcagccac catgacatcc ggtgagctgt ccggcgcagt aatacgtggt
     7621 gtttttgatg accctgaaaa tatcagctat gccggacagg gcgtgcgcgt tgaaggctcc
     7681 agcccgtccc tgtttgtccg gactgatgat gtgcggcaac tgcggcgtgg agacacgctg
     7741 accatcggtg aggaaaactt ctggatagac cgggtttcgc cggatgatgg tggaagctgt
     7801 catctctggc ttgggcgggg cgtaccgcct gccgttaacc gtcgccgctg aaagggggat
     7861 gtatggccat aaaaggtctt gagcaggccg ttgaaaacct cagccgtatc agcagaacgg
     7921 cggtgcccgg tgccgccgca atggccatta accgcgttgc ttcatccgcg atatcgcagt
     7981 cggcgtcaca ggttgcccgt gagacaaagg tacgccggaa actggtaaag gaaagggcca
     8041 ggctgaaaag ggctacggtc aaaaatccgc aggccagaat caaggttaac cggggggatt
     8101 tgcccgtaat aaagctgggt aacgcgcgga ttgtcctgtc ccgacgcagg cgtcgtaaaa
     8161 aggggcagcg ttcagccctg aaaggtggcg gcagcgtgct tgtggtgggt aaccgtcgta
     8221 ttcccggcgc gtttattcag caactgaaaa atggccggtg gcatgtcatg cagcgtgtgg
     8281 ccgggaaaaa ccgttacccc attgatgtgg tgaaaatccc gatggcggtg ccgctgacca
     8341 cggcgtttaa acaaaatatt gagcggatac ggcgtgaacg tcttccgaaa gagctgggct
     8401 atgcgctgca gcatcaactg aggatggtaa taaagcgatg aaacatactg aactccgtgc
     8461 agccgtactg gatgcactgg agaagcatga caccggggcg acgttttttg atggtcgccc
     8521 cgctgttttt gatgaggcgg attttccggc agttgccgtt tatctcaccg gcgctgaata
     8581 cacgggcgaa gagctggaca gcgatacctg gcaggcggag ctgcatatcg aagttttcct
     8641 gcctgctcag gtgccggatt cagagctgga tgcgtggatg gagtcccgga tttatccggt
     8701 gatgagtgat atcccggcac tgtcagattt gatcaccagt atggtggcca gcggctatga
     8761 ctaccggcgc gacgatgatg cgggcctgtg gagttcagcc gatctgactt atgtcattac
     8821 ctatgaaatg tgaggacgct atgcctgtac caaatcctac aatgccggtg aaaggtgccg
     8881 ggaccacact gtgggtttat aaggggaacg gtgaccctta tgcgaacccg ctttcagacg
     8941 ttgactggtc gcgtctggct aaagttaaag acctgacgcc cggcgaactg accgctgagt
     9001 cctatgacga cagctatctc gatgatgaag atgcggactg gactgcgacc gggcaggggc
     9061 agaaatctgc cggagatacc agcttcacgc tggcgtggat gcccggagag caggggcagc
     9121 aggcgctgct ggcgtggttt aatgaagggg atacccgtgc ctataaaatc cgcttcccga
     9181 acggcacggt cgatgtgttc cgcggctggg tcagcagtat cggtaaggcg gtgacggcga
     9241 aggaagtgat cacccgtacg gtgaaagtca ccaacgtggg acgtccgtcg atggcagaag
     9301 atcgcagcac ggtaacagcg gcaaccggca tgaccgtgac gcctgccagc acttcggtgg
     9361 tgaaagggca gagcaccacg ctgaccgtgg ccttccagcc ggagggcgta accgacaaga
     9421 gctttcgtgc ggtgtctgcg gataaaacaa aagccaccgt gtcggtcagt ggtatgacca
     9481 tcaccgtgaa cggcgttgct gcaggcaagg tcaacattcc ggttgtatcc ggtaatggtg
     9541 agtttgctgc ggttgcagaa attaccgtca ccgccagtta atccggagag tcagcgatgt
     9601 tcctgaaaac cgaatcattt gaacataacg gtgtgaccgt cacgctttct gaactgtcag
     9661 ccctgcagcg cattgagcat ctcgccctga tgaaacggca ggcagaacag gcggagtcag
     9721 acagcaaccg gaagtttact gtggaagacg ccatcagaac cggcgcgttt ctggtggcga
     9781 tgtccctgtg gcataaccat ccgcagaaga cgcagatgcc gtccatgaat gaagccgtta
     9841 aacagattga gcaggaagtg cttaccacct ggcccacgga ggcaatttct catgctgaaa
     9901 acgtggtgta ccggctgtct ggtatgtatg agtttgtggt gaataatgcc cctgaacaga
     9961 cagaggacgc cgggcccgca gagcctgttt ctgcgggaaa gtgttcgacg gtgagctgag
    10021 ttttgccctg aaactggcgc gtgagatggg gcgacccgac tggcgtgcca tgcttgccgg
    10081 gatgtcatcc acggagtatg ccgactggca ccgcttttac agtacccatt attttcatga
    10141 tgttctgctg gatatgcact tttccgggct gacgtacacc gtgctcagcc tgtttttcag
    10201 cgatccggat atgcatccgc tggatttcag tctgctgaac cggcgcgagg ctgacgaaga
    10261 gcctgaagat gatgtgctga tgcagaaagc ggcagggctt gccggaggtg tccgctttgg
    10321 cccggacggg aatgaagtta tccccgcttc cccggatgtg gcggacatga cggaggatga
    10381 cgtaatgctg atgacagtat cagaagggat cgcaggagga gtccggtatg gctgaaccgg
    10441 taggcgatct ggtcgttgat ttaagtctgg atgcggccag atttgacgag cagatggcca
    10501 gagtcaggcg tcatttttcc ggtacggaaa gtgatgcgaa aaaaacagcg gcagtcgttg
    10561 aacagtcgct gagccgacag gcgctggctg cacagaaagc ggggatttcc gtcgggcagt
    10621 ataaagccgc catgcgtatg ctgcctgcac agttcaccga cgtggccacg cagcttgcag
    10681 gcgggcaaag tccgtggctg atcctgctgc aacagggtgg tcaggttaag gactccttcg
    10741 gcgggatgat ccccatgttc agggggcttg ccggtgcgat caccctgccg atggtggggg
    10801 ccacctcgct ggcggtggcg accggtgcgc tggcgtatgc ctggtatcag ggcaactcaa
    10861 ccctgtccga tttcaacaaa acgctggtcc tttccggcaa tcaggcggga ctgacggcag
    10921 atcgtatgct ggtcctgtcc agagccgggc aggcggcagg gctgacgttt aaccagacca
    10981 gcgagtcact cagcgcactg gtcaaggcgg gggtaagcgg tgaggctcag attgcgtcca
    11041 tcagccagag tgtggcgcgt ttctcctctg tatccggcgt ggaggtggac aaggtcgctg
    11101 aagccttcgg gaagctgacc acagacccga cgtcggggct gacggcgatg gcacgccagt
    11161 tccataacgt gacggcggag cagattgcgt atgttgctca gttgcagcgt tccggcgatg
    11221 aagccggggc attgcaggcg gcgaacgagg ccgcaacgaa agggtttgat gaccagaccc
    11281 gccgcctgaa agagaacatg ggcacgctgg agacctgggc agacaggact gcgcgggcat
    11341 tcaaatccat gtgggatgcg gtgctggata ttggtcgtcc tgataccgcg caggagatgc
    11401 tgattaaggc agagactgcg tttaagaaag cagacgacat ctggaatctg cgcaaggatg
    11461 attattttgt taacgatgaa gcgcgggcgc gttactggga tgatcgtgaa aaggcccgtc
    11521 ttgcgcttga agccgcccga aagaaggctg agcagcagac tcaacaggac aaaaatgcgc
    11581 agcagcagag cgataccgaa gcgtcacggc tgaaatatac cgaagaggcg cagaaggctt
    11641 acgaacggct gcagacgccg ctggagaaat ataccgcccg tcaggaagaa ctgaacaagg
    11701 cactgaaaga cgggaaaatc ctgcaggcgg attacaacac gctgatggcg gcggcgaaaa
    11761 aggattatga agcgacgctg aaaaagccga aacagtccgg cgtgaaggtg tctgcgggcg
    11821 atcgtcagga agacagtgct catgctgacc tgctgacgct tcaggcagaa ctccggacgc
    11881 tggagaagca tgccggagcg aatgagaaaa tcagccagca gcgccgggat ttgtggaagg
    11941 cggagagtca gttcgcggta ctggaggagg cggcgcaacg tcgccagctg tctgcacagg
    12001 agaaatctct gctggcgcat aaagatgaga cgctggagta caaacgccag ctggctgcac
    12061 ttggcgacaa ggtcacctat caggagcgcc tgaacgcgct ggcgcagcag gcggataaat
    12121 tcgcacagca gcaacgggca aaacgggccg ccattgatgc gaaaagccgg gggctgactg
    12181 accggcaggc agaacgggaa gccacggaac agcgcctgaa ggaacagtat ggcgataatc
    12241 cgctggcgct gaataacgtc atgtcagagc agaaaaagac ctgggcggct gaagaccagc
    12301 ttcgcgggag ctggatgaca ggcctgaagt ccggctggag tgagtgggaa gagagcgcca
    12361 cggacagtat gtcgcaggta aaaagtgcag ccacgcagac ctttgatggt attgcacaga
    12421 atatggcagc gatgctgacc ggcagtgagc agaactggcg cagcttcacc cgttccgtgc
    12481 tgtccatgat gacagaaatt ctgcttaagc aggcaatggt ggggattgtc gggagtatcg
    12541 gcagcgccat tggcggggct gttggtggcg gcgcatccgc gtcaggcggt acagccattc
    12601 aggccgctgc ggcgaaattc cattttgcaa ccggaggatt cacaggaacc ggcggcaaat
    12661 atgagccagc ggggattgtt caccgtggtg aatttgtctt cacgaaggag gcaaccagcc
    12721 ggattggcgt ggggaatctc taccggctga tgcgcggcta tgccaccggt ggttatgtcg
    12781 gtacaccggg cagtctggct gacagccggt cgcaggcgtc cgggacgttt gagcagaata
    12841 accatgtggt gattaacaac gacggcacga acgggcagat aggtccggct gctctgaagg
    12901 cggtgtatga catggcccgc aagggtgccc gtgatgaaat tcagacacag atgcgtgatg
    12961 gtggcctgtt ctccggaggt ggacgatgaa aaccttccgc tggaaagtga aacccggtat
    13021 ggatgtggct tcggcccctt ctgtaagaaa ggtgcgcttt ggtgatggct attcccagcg
    13081 agcgcctgcc gggctgaatg ccaacctgaa aacgtacagc gtgacgcttt ctgtcccccg
    13141 tgaggaggcc acggtactgg agtcgtttct ggaagagcac ggtggctgga aatcctttct
    13201 gtggacgccg ccttatgagt ggcggcagat aaaggtgacc tgcgcaaaat ggtcgtcgcg
    13261 ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa caggtggtga actgatgcag
    13321 gatatccggc aggaaacatt gaatgaatgc acccgtgcgg agcagtcggc cagcgtggtg
    13381 ctctgggaaa tcgatctgac agaggtcggt ggagaacgtt attttttctg taatgagcag
    13441 aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta tcccattcag
    13501 gggagtggtt ttgaactgaa tggcaaaggc accagtacgc gccccacgct gacggtttct
    13561 aacctgtacg gtatggtcac cgggatggcg gaagatctgc agagtctggt cggcggaacg
    13621 gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac
    13681 agtgacgccg atccggagca ggaggtgatc agccgctggc gcatcgagca gtgcagcgaa
    13741 ctgagcgcgg tgagtgcctc ctttgtactg tccacgccga cggaaacgga tggcgctgtt
    13801 tttccgggac gtatcatgct ggccaacacc tgcacctgga cctatcgcgg tgacgagtgc
    13861 ggttatagcg gtccggctgt cgcggatgaa tatgaccagc cgacgtccga tatcacgaaa
    13921 gataaatgca gcaaatgcct gagtggctgt aagtttcgca ataacgtcgg caactttggc
    13981 ggcttccttt ccattaacaa actttcgcag taatcccatg acagagacag aatcagcgat
    14041 tctggcgcac gcccggcgat gtgcgccagc ggagtcgtgc ggcttcgtgg taagcacgcc
    14101 ggagggggaa agatattttc cctgcgtgaa tatttccggt gagccggagg attatttccg
    14161 gatggctccg gaggactggc tgcaggccaa aatgcagggt gagattgtgg cgctggtcca
    14221 cagtcacccc ggtggtctgc cctggctgag tgaggccgac cggcggctgc aggtgcagag
    14281 tgatttgccg tggtggctgg tctgccgggg agtgattcat aagttccgct gtgtgccgca
    14341 tctcaccggg cggcgctttg agcacggggc gacggactgt tacacgctgt tccgggatgc
    14401 ttatcatctg gctgggattg agatgccgga ttttcatcgc ggggatgact ggtggcgtca
    14461 cggtcagaat ctctatcttg acaatatgga ggcgactggt ttttaccgtg tcgcactgac
    14521 agaggcgcag ccgggcgatg tgctgctgtg ctgctttggt tcatcggtgc cgaatcatgc
    14581 cgctatttac tgcggtgacg gcgagctgct gcaccatatt cctgaacaac tgagcaaacg
    14641 agagaggtat accgacaaat ggcagcgacg cacacactcc ctctggcgtc accgggcatg
    14701 gcgcgcatct gcctttacgg ggatttacaa cgatttggcc gccgcatcga ccttcgtgtg
    14761 aaaacggggg ctgaagccat ccgggcactg gccacacagc tcccggcgtt tcgtcagaaa
    14821 ctgtgcgacg gctggtatca ggtacggatt gccgggcggg acgtcagcac gtccgggtta
    14881 acggtgcagt tacatgagac tctgcctgat ggcgctgtga ttcatattgt tcccagagtc
    14941 gccggggcca agtcaggtgg cgtattccag attgtcctgg gagctgccgc cattgccgga
    15001 tcattcttta ctgccggagc cacccttgca gcatgggggg cagccattgg ggccggtggt
    15061 atgaccggca tcctgttttc tctcggtgcc agtatggtgc t
//
LOCUS       DAZKFU020000110        15063 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZKFU020000110 DAZKFU020000000
VERSION     DAZKFU020000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.111"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(105..2384)
                     /gene="maeB"
                     /locus_tag="ACHOQQ_RS13565"
                     /old_locus_tag="pgaptmp_002713"
     CDS             complement(105..2384)
                     /gene="maeB"
                     /locus_tag="ACHOQQ_RS13565"
                     /old_locus_tag="pgaptmp_002713"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGU4470942.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2673..3623
                     /gene="tal"
                     /locus_tag="ACHOQQ_RS13570"
                     /old_locus_tag="pgaptmp_002714"
     CDS             2673..3623
                     /gene="tal"
                     /locus_tag="ACHOQQ_RS13570"
                     /old_locus_tag="pgaptmp_002714"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4470943.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3643..5646
                     /gene="tkt"
                     /locus_tag="ACHOQQ_RS13575"
                     /old_locus_tag="pgaptmp_002715"
     CDS             3643..5646
                     /gene="tkt"
                     /locus_tag="ACHOQQ_RS13575"
                     /old_locus_tag="pgaptmp_002715"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4470944.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5741..6784)
                     /gene="ypfG"
                     /locus_tag="ACHOQQ_RS13580"
                     /old_locus_tag="pgaptmp_002716"
     CDS             complement(5741..6784)
                     /gene="ypfG"
                     /locus_tag="ACHOQQ_RS13580"
                     /old_locus_tag="pgaptmp_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGU4470945.1"
                     /translation="MRYRNLLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6910..7485)
                     /gene="nudK"
                     /locus_tag="ACHOQQ_RS13585"
                     /old_locus_tag="pgaptmp_002717"
     CDS             complement(6910..7485)
                     /gene="nudK"
                     /locus_tag="ACHOQQ_RS13585"
                     /old_locus_tag="pgaptmp_002717"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGU4470946.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7553..9532)
                     /gene="aegA"
                     /locus_tag="ACHOQQ_RS13590"
                     /old_locus_tag="pgaptmp_002718"
     CDS             complement(7553..9532)
                     /gene="aegA"
                     /locus_tag="ACHOQQ_RS13590"
                     /old_locus_tag="pgaptmp_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGU4470947.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9738..11438
                     /gene="narQ"
                     /locus_tag="ACHOQQ_RS13595"
                     /old_locus_tag="pgaptmp_002719"
     CDS             9738..11438
                     /gene="narQ"
                     /locus_tag="ACHOQQ_RS13595"
                     /old_locus_tag="pgaptmp_002719"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGU4470948.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11602..14715
                     /gene="acrD"
                     /locus_tag="ACHOQQ_RS13600"
                     /old_locus_tag="pgaptmp_002720"
     CDS             11602..14715
                     /gene="acrD"
                     /locus_tag="ACHOQQ_RS13600"
                     /old_locus_tag="pgaptmp_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGU4470949.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14814..14873)
                     /gene="ypfM"
                     /locus_tag="ACHOQQ_RS13605"
                     /old_locus_tag="pgaptmp_002721"
     CDS             complement(14814..14873)
                     /gene="ypfM"
                     /locus_tag="ACHOQQ_RS13605"
                     /old_locus_tag="pgaptmp_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGU4470950.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3535 a         3988 c         3918 g         3622 t
ORIGIN      
        1 tgcttttttt attattcgca aactcagtaa taagctttac taatatattg cagccaaacc
       61 gcttaccctg aatattcagg gtaagcgtga gagttaaaaa agaattacag cggttgggtt
      121 tgcgcttcaa ccacggccag cgccaccatg ttgacgatac gacgcaccga tgcgatcggc
      181 gttaacacgt gaaccggttt cgccacgccc atcagcaccg gaccgacagt cacaccttcc
      241 gagctggaaa cgcgcagtaa gttgtaacta atgcgggcag cttccatgtt cggcatcacc
      301 agaatattgg cggaaccttt caaagggctg tccggcatac ggtcgttgcg aatcgcttcc
      361 accagcgctg catcgccgtg catttcacca tcaatcatca gttctggtgc acgttccctg
      421 accagttcca gcgcctgacg cattttgctc gacgacgggc agtcagaaga accaaagttg
      481 gagtgcgaca acaaagcaac gcgcggttca ataccgaaac gacggacagt ttctgccgcc
      541 atcaaggtga tctccgccag ctcttctgcg tctggttcgt cattgacgta ggtatcggca
      601 ataaaggtgt taccactcgg cagcagcagc gcgttcattg cacctgcggt gtgaacgcca
      661 tcgcgataac caaagacatt tttcaccacg ctaaaatgtt catgataatc gcccaccgta
      721 ccgcagatca ttgcatcggc ttccccacgc tgaaccatga tcgcgccgat cactgtcggg
      781 ttactgatca gcgcacgctg cgcctgttcc tgagtgacac cgcgacgctt catgatctgg
      841 aagtattcgg tccagtactc tttaaagcgc ggatcggatt cgttattgac gatctcaaaa
      901 tcaacgcccg ctttgatctg caagcccagt ttctgaatgc gcatttcgat cacgttcgga
      961 cgaccgataa ggatcggttt cgccagtccc agcgttacca gttcctgagt ggcatgcaga
     1021 acgcgcgcct cttccccttc cggcagaact acgcgcttcg gcgctttgcg agcctgggag
     1081 aaaatcggct tcataaacag gttggttttg taaacgaact cagtcagctt gtcgatgtag
     1141 acgtcgaaat cagcaatcgg acgcgtcgcc acgcccgact ccatcgccgc tttcgcgacc
     1201 gcaggagcga tcttaacgat caagcgcgga tcaaacggtt ttggaatgat gtattccgga
     1261 ccaaagctca gatcctgatc gccatacgct gaagccacca cttcgctctg ttccgcatgg
     1321 gccagttctg caatcgcgcg taccgccgcc agtttcatct cttcgttgat ggcggttgcg
     1381 ccaacgtcca gcgcgccacg gaagatgaac gggaagcaca ggacgttgtt cacctggttt
     1441 ggatagtcag aacgaccggt gcagatgatg gcatccggac gcacttcttt cgccagcggc
     1501 ggcagaattt ccggttccgg gttcgccagc gccaggatca ttggcgcacg agccattttc
     1561 ttcaccattt cctgggtcag cactttcggg ccggaacagc cgaggaaaat atccgcgcct
     1621 tcaatcacat catcgagggt acgtttgccg tcatccacca ccgcatatgc ggctttggtt
     1681 tccgccatgt ttggctcacg gccctgatag ataacgcctt ttgaatcgca aaccacgatg
     1741 ttatgttttt gcagacccag cgcgaccagc aggttcatac aggcgattgc tgcggcgccc
     1801 gcgccggaaa ccaccatccg cacgtcagag atgtttttct ccaccacgcg caagccgttg
     1861 aggatggcgg cagtgctgat aattgccgtg ccgtgctgat cgtcgtggaa taccggaata
     1921 ttcatccgct cgcgcagttt ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg
     1981 aggttgatgc cgccgaaggt cggttcgagc gcagcgacga cttcaataaa tttgtccggg
     2041 tcgagttcat caacttcaat gtcaaataca tcaatcccgg cgaatttctt aaacagaacg
     2101 cccttgcctt ccatcaccgg tttgcctgcc agcgcgccaa tgttgcctaa ccccagcacc
     2161 gccgtaccgt tagagatcac cgccaccagg ttaccgcgcg cggtatattt gtaggctttt
     2221 aacgggtctt tttcgatttc aagacaaggt gcggcaacgc ctggtgagta ggccagcgcc
     2281 agatcgcgct gcgttgccag aggcttggtt ggagaaacct ggattttccc tggaactgga
     2341 aattcatgga aatcaagtgc actttgtttt aactggtcat ccatttggtt gttcctttca
     2401 cgtaacgttc acaaataaag tgtgtgggca acagcccctg cccacaacgt ggcgcacatt
     2461 attaccttgc cggagtctac agactttgag caagtccaaa ctctcaccat taatataatg
     2521 ttttggtaat aatcctataa cactgatgtt acctgcttaa tccagcaata ccatgcctgt
     2581 ctgctatgct tttttgatgc gtttagcgaa atttctcaga agtgtgaatt aacgcactca
     2641 tctaacactt tacttttcaa ggagtatttc ctatgaacga gttagacggc atcaaacagt
     2701 tcaccactgt cgtggcagac agcggcgata ttgagtccat tcgccattat catccccagg
     2761 atgccaccac caatccttcg ctgttactca aggctgccgg attatcacaa tatgagcatt
     2821 taatagacga tgctatcgcc tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg
     2881 tcgcagcgtg tgacaaactg gcggtcaatt tcggtgctga aatcctcaaa atcgtacccg
     2941 gtcgcgtgtc aacagaagtt gatgcacgcc tctcttttga taaagaaaag agtattgaga
     3001 aggcgcgcca tctggtggac ttgtatcagc aacaaggcgt tgagaaatca cgcattctga
     3061 tcaaactggc ttcgacctgg gaaggaattc gcgcggcaga agagctggaa aaagaaggta
     3121 ttaactgcaa cctgacgctg ctgttttctt ttgcacaggc acgggcctgt gcagaagcag
     3181 gcgtttttct gatttcgccg tttgtcgggc gtatttatga ctggtatcag gcacgcaagc
     3241 cgatggaccc gtatgtggtg gaagaagatc caggcgttaa atcagtacgc aatatctacg
     3301 actactataa gcaacaccac tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg
     3361 aacaaatcct cgccttaacc ggctgcgatc gactgactat cgcaccgaat ttgctgaagg
     3421 agctgcagga aaaagtttcg ccagtggtac gtaaattaat cccaccttct cagacgttcc
     3481 cacgcccagc tcccatgagc gaagcggagt tccgttggga gcacaatcag gatgcgatgg
     3541 cggtagaaaa actgtctgaa ggcattcgtc tgttcgccgt tgatcaacgc aaactggaag
     3601 atcttcttgc cgccaaacta taaaccagcc acggagtgtt atatgtcccg aaaagacctt
     3661 gccaatgcga ttcgcgcact cagtatggat gcggtacaaa aagccaattc tggtcatccc
     3721 ggcgcgccga tgggcatggc tgatattgca gaagtgctgt ggaacgattt tcttaaacat
     3781 aaccctaccg acccaacctg gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg
     3841 tcgatgctgc tctacagttt gctacatctg accggttacg acctgccgct ggaagaactg
     3901 aagaacttcc gtcagttgca ttcgaaaacc cctggccacc cggaaatcgg ctatacgccc
     3961 ggtgttgaaa ccaccaccgg tccacttgga caaggtttgg cgaacgccgt cggactggcg
     4021 atagcagagc gtacactggc ggcgcagttt aaccagccgg atcatgaaat tgtcgatcac
     4081 ttcacctatg tgtttatggg cgacggctgc ctgatggaag gtatttccca cgaagtctgt
     4141 tcgctggcgg gcacgctggg actgggcaag ctgattggtt tttacgatca caacggtatt
     4201 tcgattgatg gagaaaccga aggctggttt accgacgata cggcaaaacg ttttgaagcc
     4261 tatcactggc atgtgatcca tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg
     4321 atccttgaag cgcaaagcgt gaaagataag ccgtcgctga ttatctgccg tacggtgatt
     4381 ggctttggtt cgccgaataa agcaggtaag gaagaggcgc acggcgcacc gctgggggaa
     4441 gaagaagtgg cgctggcacg gcaaaaactg ggctggcacc atccgccatt tgagatccct
     4501 aaagatattt atcacgcctg ggatgcccgc gaaaaaggcg aaaaagcgca gcagagctgg
     4561 aatgagaagt ttgccgccta taaaaaggct catccgcaac tggcagaaga gtttacccgt
     4621 cggatgagcg gtggtttacc gaaggactgg gagaaaacga ctcagaaata tatcaatgag
     4681 ttacaggcaa atccggcgaa aatcgccacc cgtaaggctt cgcaaaatac gcttaacgct
     4741 tacggaccga tgctaccgga gctgctcggc ggttcggcgg atctggctcc cagcaacctg
     4801 accatctgga aaggctctgt gtcgctgaag gaagatccgg cgggaaatta tattcactac
     4861 ggggtgcgtg aatttggcat gaccgctatc gccaacggca ttgcgcacca cggcggcttt
     4921 gtgccgtata ccgcgacatt cctgatgttt gttgaatacg cccgtaatgc cgcgcggatg
     4981 gcggcactga tgaaagcacg gcagattatg gtttataccc acgactcaat tggcctgggc
     5041 gaagatggtc cgacgcacca ggctgttgag caactggcca gcctgcgctt aacgccaaat
     5101 ttcagcacct ggcgaccgtg cgatcaggtg gaagcggcgg tgggctggaa gctggcggtt
     5161 gagcgccaca acggaccgac ggcactgatt ctctccaggc agaatctggc ccaggtggaa
     5221 cgtacgccgg atcaggttaa agagattgct cgtggcggtt atgtgctgaa agacagcggc
     5281 ggtaagccag atattatttt gattgccacc ggttcagaga tggaaatcac cctgcaagcg
     5341 gcagagaaat tagcgggaga aggtcgcaat gttcgcgtag tttccctgcc ctcgaccgat
     5401 attttcgacg cccaggatga ggaatatagg gagtcggtgt tgccttctaa cgttgcggct
     5461 cgcgtggcgg tggaagcagg tattgccgat tactggtaca agtatgttgg tctgaaaggg
     5521 gcaattgtcg ggatgacggg ttacggggaa tctgctccgg cggataagct gttcccgttc
     5581 tttggcttta ccgccgagaa tattgtggca aaagcgcata aggtgctggg agtgaaaggt
     5641 gcctgatggt gatcgccgga tgctgattgc cggatgcgac gctgacgcgt cttatccggc
     5701 ctacagcgtc ttatccggcc tacatgtccc gccattttgt ttaacgggtg atccacaacg
     5761 tgggccaggc gtctggccca tgccagttat cgcaggtggg ttctgcggcg taacgcacca
     5821 ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg cttaatccgc
     5881 gccctttcgc taaggtcacc agttcacgcg atttctcatc aaatgttgcg ttcatcagtt
     5941 ccagttcatt tgtctcctgc ccgttgttga acggcaaacg caaccgaacc ggacgcgagg
     6001 ctagtggctt tttacgcgac acaatccatg ccaaatcaat ggtgttatag gcccctgcct
     6061 cacagctaat catcatcagc gctttatcat cagtcagcgc agtgacattc acctcacgac
     6121 gtaacggatc aagcgagcag cgcagaccat tcatccgcca gtttccataa tccagcaaat
     6181 cgttacgttc ttcgagtgag agtggcgtcg gcgttgggtt aaccaccgcg acctctttca
     6241 acgcaggcgc tggcggtacg ctgagcggcg gttcgtcccc tttctttatc cacgcggttt
     6301 cactgccaac gcgcttttgc tgagcatcaa taaacaacaa cgccgctttt aagccactca
     6361 gagaaatggt ctgattgccg tcgcgtaagg tgatcgcctt cccttcctga atcatctgca
     6421 aaaacgcggt gatggttgcc gtatcatcgg ttactaataa ccatggtgaa atccgccact
     6481 tgtcaccact taacgccaac ggctcaccat ctaacagcag ccgtggcgct atctcccctt
     6541 cagacgcatc cggcgatttc aacccgccgc gctcaatacg taaaacggca tcggtatgcg
     6601 ccccggcgct gcggctcagg gtcatcacca gtccattatg atcgcccgta ttgcgcgcca
     6661 cgcagaagtt ttggttatta caggtgacct gccagtcgga aaacgcccgt tgcgctggtg
     6721 ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaga ttgcgatagc
     6781 gcatggacgg tacgacccca gaagttcaaa caaacagcac aagtcgtatc ttcgttgcgc
     6841 aggtagcgtg gctcaatcgg atttgtcgga ttgagccaat aataaacaac gaaatattat
     6901 tgttattttt cagtccatta aatgtgacgt ttgcaaatag ttaagcaata acaccgtctt
     6961 accgtcacgt atctcgccgg ttttgatcat ctccagcgcc tggctgaacg gcagctcgag
     7021 cacttcaata tcttcatctt cgacaccgcc accggcgtta gcgcgctgat tgtcactgta
     7081 ttcggcgata aaaaagtgga ttagctcagt cacaccgcct ggcgacatat acagttcaaa
     7141 taatttgcgc acttcgccca cttcatagcc cgtctcttca atcgcttctt tacgaatgca
     7201 cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagct gcccgctttc
     7261 attgccatta acccaggtag cgacacggaa ctgacgaatc agaaccacgg tctttttctt
     7321 cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg taaacttcac gtttatggcg
     7381 gataacttcg ccatctttgc gcgtgagatc gtaagtaatg ttgtgcaggg tgaaatagtt
     7441 atcggagaga attttgtctt taatgagggt gatttgttgc gtcataccga ctccacagcg
     7501 cgaaatgaac aattatatta cgctgtgaag tcgggtttgt ctgcgcaggc tatcagtgag
     7561 atttgactga ttttaccccc agccagtcaa taatcccctg tgccgcatga cgtccttctg
     7621 ccattgcggt aaccaccaga tccgcaccac gcacggcgtc accaccagcg aagattttcg
     7681 gattggtggt ctggtaacgg tactggcttt ccacatccgc gatgatgcgg ccccatttgt
     7741 ctaccgttac accgtgcgac tccagccacg gcatcccgtg cggattgaag ccaaacgcca
     7801 taatcaccgc gtcggctggc atgacaaatt cactgccttc caccggcact ggacgcctac
     7861 gcccctgggc atccggctcg ccaagacgcg tacgcaggaa acgaatcccg caaacgtgac
     7921 cttgttcatt cagctcaagc gccaccggct ggacgttaaa ttcgaagttg gccccctctt
     7981 cgcgggcgtt cttcacctct ttcttcgagc ctggcatgtt agcttcatca cgacgataag
     8041 cgcaggtgac gttactcgcg ccgtgacgca gcgcagtacg cacacagtcc atcgcggtgt
     8101 cgccgccgcc cagtaccacg acgttaagtc cggcggtatt gataaacggc tcttccggta
     8161 gctcttcgag ccccatcacc tgtttagtgt tggcgatgag gaacggcagc gcgtcataaa
     8221 cgcccggcgc atcttcattg ggtaaacccg ctttcatgga acggtaagtg cctacgccaa
     8281 cgaagaccgc gtcgtattgt tccaaaagcg aatccaaaga gacatcttta cccacttcac
     8341 aattgagttc gaagtgaatc cccatcgcgc tgaagatttc ccgacggcgt gccagcaggg
     8401 atttatccag tttgaaagaa ggaatgccga aagtgagcaa gccaccgatt tctggatggc
     8461 gatcgtacac cgtcaccgcc acgccattgc gggtcagaac atccgcacag gccagccctg
     8521 ccggacctgc accgataatc gccacccgct tgtccacttt ggtgacatgg cttaagtcag
     8581 gacgccaacc tttcgccaac gcctgatctg aaatgtagcg ttcaatgttg ccgatagtta
     8641 ccgcaccgtg ctcatcgcga atagtacagg caccttcaca caaacggtct tgcggacaaa
     8701 cgcgtccggt aatttccggc agggtgttgg tcaggtgaga aagctcgacg gcggcgtcga
     8761 tgtttccggc tttcaccagt tcaatccact gcggtatatg gttatgcagc gggcaggtcc
     8821 attcacaaac gctgtgctcg ccgcatttaa ggcagcgcga ggcttcccgt tgtgcctgat
     8881 cggcacggaa tggcagataa atttcatcaa aaccggtttt gcgcgcttca atcgccagtt
     8941 tatccggctc gccacgcgcg ggcgttgcct gcatttgctc gactttactc attaccggca
     9001 tttcttgcgc cgcggtactg gcatgccacg gttgatgttc ctgacgtgcg gtacgcaagc
     9061 ggcgggattt cgccatgcct gagagtgcgg cgtcagtgac cagctgcagc gcgtccgccg
     9121 ggcaattctc gacacaggca ggaccgtttt cgcgccctgc acaaaggtcg catttatgcg
     9181 ccgtggcttt aactttgccc gctgcgacgg gtgtcaggac gatttgcatc gtaccaaacg
     9241 gacaggccac cacgcaggat ttacagccaa tacacttttg ctgattgacc tgaatgctgt
     9301 catcaacgtg gctgattgcg ccattagggc agctacgggc gcagggcgca tcttcacaat
     9361 ggtgacaggt cactgcacta cgttgctgct gatgtttgat aaccgtaatt cggggatgaa
     9421 aatgatgttg gctcaggaca tgttgctcat cattgtgagc catgacacaa gcgatttcac
     9481 aagcatgaca acccagacat tgctgactgt tggccataat aaaacgattc ataacgacct
     9541 tcttttttgg ttgtaaaaac cttattcttt atatgagtgt tgttattacc cgacttacag
     9601 gggaatcggc aatgttcatg tgcccagaat aagtaactat ttcgctataa actgtggcag
     9661 atcaaataat cccatcactg actaaattgc gtttcagcga actggaacat taatgatttt
     9721 ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc ggccagtctg gcccgggcct
     9781 ttttttacat tgtgctgctg tcgattcttt ccacgggtat cgctctgcta actctggcga
     9841 gcagtttgcg cgacgctgag gctatcaata ttgccggatc gctgcgtatg cagagttacc
     9901 gcctgggcta cgacttgcaa agtggcagtc cacaactcaa tgcacatcgc cagttatttc
     9961 agcaggcact gcattcaccg gtattaacca acctcaacgt ctggtatgtg ccagaagcag
    10021 taaaaactcg ctatgcgcat ctgaatgcca actggctgga gatgaataat cggctcagca
    10081 agggcgattt gccgtggtat caggccaata tcaataatta tgttaatcag atagacctgt
    10141 tcgtgctggc tttacagcac tacgctgaac gcaaaatgct gctggtggtg gcgatttccc
    10201 tggctggcgg catcggtatt ttcacgctgg tcttttttac tttgcgccgc atacgccatc
    10261 aggtggttgc cccgctgaat cagctggtta ccgccagtca gcgtattgaa cacgggcagt
    10321 tcgactcgcc accgctggat accagcctgc cgaatgagct tggtctgctt gcaaaaacct
    10381 ttaaccagat gtcgagcgag ctgcataaat tgtacctttc gctggaggcg tcagtagaag
    10441 aaaagacccg cgatctccac gaggccaagc gtcgtctgga ggtgttgtat caatgttcgc
    10501 aggcgctgaa caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc
    10561 gcgataatga agcggctgaa tatctggagt taaatgtcgg tgaaaactgg cggattagcg
    10621 aagggcaacc aaacccggaa ttgccgatgc agattttacc ggtgacaatg caagagacgg
    10681 tttacggcga actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca
    10741 gcgtttcgtc gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc
    10801 agttattgtt gatggaagaa cgtgcaacca tcgcccgcga attgcacgac tcgctggctc
    10861 aggtactttc ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata
    10921 acgccaccgc acaaagtatc atggccgatt tttcccaggc gttgaatgat gcttatcggc
    10981 agttacgcga gctgttgact acttttcgcc tgacgctgca gcaggcggat ctcccctccg
    11041 cgttgaggga aatgctggat acgttacaaa atcaaaccag cgccaaactg accctcgact
    11101 gccgtctgcc aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc
    11161 gcgaagcggt gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg
    11221 tcaccgcgcc ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg
    11281 aaccgaaaga acccgaaggt cattatggtc tgaatatcat gcgcgaacgc gcggaacggc
    11341 taggtgggac gctgactttt tcacaacctt ccggcggcgg tacgttagtg agtattagct
    11401 ttcgctctgc ggagggtgag gaaagtcagt taatgtaatg cctcctactg accaaagaat
    11461 gcttgcactt aaggttcagt ataaaagggc atgataattt acattaactc ctttttttct
    11521 ccacgattgg ctcgtacctt gccgctacag tgaagcaagt caagcctaca acgatacgca
    11581 gaaacacgag gtcctctttt aatggcgaat ttctttattg atcgccccat ttttgcctgg
    11641 gtgctggcaa tcctgttgtg tctgacaggt accctggcga ttttttcatt gcccgttgaa
    11701 caataccccg atctcgcgcc accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg
    11761 gcccagacgc tggaaaacac cgtgacccag gttatcgagc aaaatatgac tggcctcgat
    11821 aatctcatgt atatgtcatc tcagagcagt ggcaccggtc aggcatctgt cactttaagt
    11881 tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca
    11941 gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc
    12001 gataccaaca ttctgaccat tgccttcgtc tctaccgatg gttcgatgga taaacaggat
    12061 attgctgatt atgttgccag taatattcag gacccgttaa gccgcgtgaa tggcgtcggg
    12121 gatatcgatg cctatggttc gcaatattcc atgcgtatct ggctggaccc ggcgaaactc
    12181 aacagtttcc agatgacggc taaagatgtc actgatgcca ttgagtcaca gaacgcgcag
    12241 attgcggttg ggcaacttgg tggtacacct tccgtcgata agcaggcgct caacgccacc
    12301 attaacgccc agtcactgct gcaaacacca gaacagttcc gcgatatcac cttgcgggtg
    12361 aatcaagacg gctcagaggt aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag
    12421 aaatacgatt atcttagccg cttcaatggt aagccagcct ccgggctggg ggtaaaactg
    12481 gcctccggcg ctaacgaaat ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg
    12541 gcgcagtatt tcccgcatgg actggaatac aaggtggcgt atgaaaccac ctcgtttgtt
    12601 aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc
    12661 gttatgtatc tgttcctgca aaacttccgc gccacgctga tacccactat cgccgtgccg
    12721 gtggtgttga tgggaacctt ctccgtactt tacgccttcg gttacagcgt caacacctta
    12781 accatgttcg ccatggtgct ggcgatcggt ctgctggtgg atgatgccat cgtggtggtg
    12841 gaaaacgtcg aacgtattat gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa
    12901 tcgatggggc agatccaggg ggcactggtc gggattgcga tggtgctttc ggcggtattt
    12961 gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct atcgccagtt ctctattacc
    13021 attgttgcgg cgatggtgct gtcagtactg gtagcgatga tcctcactcc ggctctgtgt
    13081 gccacgctac ttaagccact gaaaaaaggt gagcatcacg ggcaaaaagg cttttttgcc
    13141 tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt
    13201 ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc tgcttggcgg catggtgttc
    13261 ctgttcctgc gtttgccgac gtcgttctta ccgctggaag accgtggcat gtttactacc
    13321 tcggtacaat tgcccagcgg ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc
    13381 gagaaatatt acttcaccca tgaaaaagac aacatcatgt cggtgtttgc cactgttggt
    13441 tctggccctg ggggtaacgg gcaaaacgtg gcgcgaatgt ttatccgcct gaaagactgg
    13501 agcgaacgcg acagtaagac cggcacctca tttgccatta tcgagcgtgc aacaaaggcg
    13561 tttaaccaaa ttaaagaagc tcgcgttatc gccagcagcc cgccagcaat tagcggtctt
    13621 ggtagttctg caggttttga tatggagttg caggaccacg ctggagcagg tcacgatgcg
    13681 ctgatggcag cacgtaacca gttgctggcg ctggcggcgg aaaacccgga gctaacccgt
    13741 gtgcgccata acggcctcga tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa
    13801 gctcaggcgc tgggcgttgc tatcgacgat attaacgaca cactgcaaac cgcctggggt
    13861 tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca
    13921 gctgcgccgt atcgcatgct gccagatgac atcaatctct ggtatgtccg aaataaagat
    13981 ggcggcatgg tgcccttctc tgctttcgcg acctcacgct gggagacagg ttcgccgcgt
    14041 ctggaacgct ataacggtta ttctgcggtt gagattgttg gggaagccgc accgggggtc
    14101 agtaccggta cggcgatgga tattatggaa tcgttagtga agcagttgcc aaacggcttt
    14161 ggtctggagt ggacggcgat gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg
    14221 ctgtacgcta tttccttgct ggtggtattc ctgtgtctgg cggcgttgta tgaaagctgg
    14281 tcggtaccgt tctcggtaat gctggtcgtg ccgctggggg taatcggcgc gctgctggca
    14341 acctggatgc gcgggctgga aaacgacgtt tacttccagg tgggcctgtt aacggtcatt
    14401 ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa
    14461 ggccacgacc tgtttgaagc gacgctccac gcctgccgtc agcgtttacg cccgattctg
    14521 atgacctcgc tggcatttat cttcggcgta ttgccaatgg caaccagcac gggtgcaggt
    14581 tccggtggtc agcatgcggt gggtactggc gtaatgggcg ggatgatttc agccactatt
    14641 ctggctattt acttcgtgcc gctgttcttt gtgctggtgc gccgccgctt cccgctgaag
    14701 ccgcgcccgg aataagcaat aaaaaaggcg acatgctaat gtgtcgcctt tttcaacttt
    14761 ccgattaaga acctgctcag cgggttcttg ctgtttgtac tttgtctcag gaattactta
    14821 cgaagcataa cttcgataaa gtctttccag ttccccagtt cacgttcaat cataacaacc
    14881 tctcttataa ttatgggtat tctacggaaa caatataccg tggtgaagct aatttactcg
    14941 attgctgcga tgactacctc cgggggacaa accttatgta aatactatgg tcctgcggtg
    15001 ataatttgta tgtgatacac agcaacattt cgagatattc atacggcatc taatactgat
    15061 tta
//
LOCUS       DAZKFU020000111        14957 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZKFU020000111 DAZKFU020000000
VERSION     DAZKFU020000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.112"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..1427
                     /gene="chiP"
                     /locus_tag="ACHOQQ_RS13610"
                     /old_locus_tag="pgaptmp_002722"
     CDS             21..1427
                     /gene="chiP"
                     /locus_tag="ACHOQQ_RS13610"
                     /old_locus_tag="pgaptmp_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HGU4470951.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            1477..1803
                     /gene="chiQ"
                     /locus_tag="ACHOQQ_RS13615"
                     /old_locus_tag="pgaptmp_002723"
     CDS             1477..1803
                     /gene="chiQ"
                     /locus_tag="ACHOQQ_RS13615"
                     /old_locus_tag="pgaptmp_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGU4470952.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(1887..2333)
                     /gene="fur"
                     /locus_tag="ACHOQQ_RS13620"
                     /old_locus_tag="pgaptmp_002724"
     CDS             complement(1887..2333)
                     /gene="fur"
                     /locus_tag="ACHOQQ_RS13620"
                     /old_locus_tag="pgaptmp_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGU4470953.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(2326..2412)
                     /gene="uof"
                     /locus_tag="ACHOQQ_RS13625"
                     /old_locus_tag="pgaptmp_002725"
     CDS             complement(2326..2412)
                     /gene="uof"
                     /locus_tag="ACHOQQ_RS13625"
                     /old_locus_tag="pgaptmp_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGU4470954.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            complement(2622..3152)
                     /gene="fldA"
                     /locus_tag="ACHOQQ_RS13630"
                     /old_locus_tag="pgaptmp_002726"
     CDS             complement(2622..3152)
                     /gene="fldA"
                     /locus_tag="ACHOQQ_RS13630"
                     /old_locus_tag="pgaptmp_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGU4470955.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            complement(3292..3585)
                     /gene="ybfE"
                     /locus_tag="ACHOQQ_RS13635"
                     /old_locus_tag="pgaptmp_002727"
     CDS             complement(3292..3585)
                     /gene="ybfE"
                     /locus_tag="ACHOQQ_RS13635"
                     /old_locus_tag="pgaptmp_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGU4470956.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            complement(3725..4489)
                     /gene="ybfF"
                     /locus_tag="ACHOQQ_RS13640"
                     /old_locus_tag="pgaptmp_002728"
     CDS             complement(3725..4489)
                     /gene="ybfF"
                     /locus_tag="ACHOQQ_RS13640"
                     /old_locus_tag="pgaptmp_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4470957.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPTMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            4674..5219
                     /gene="seqA"
                     /locus_tag="ACHOQQ_RS13645"
                     /old_locus_tag="pgaptmp_002729"
     CDS             4674..5219
                     /gene="seqA"
                     /locus_tag="ACHOQQ_RS13645"
                     /old_locus_tag="pgaptmp_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGU4470958.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5245..6885
                     /gene="pgm"
                     /locus_tag="ACHOQQ_RS13650"
                     /old_locus_tag="pgaptmp_002730"
     CDS             5245..6885
                     /gene="pgm"
                     /locus_tag="ACHOQQ_RS13650"
                     /old_locus_tag="pgaptmp_002730"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGU4470959.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(7060..7737)
                     /gene="kdpE"
                     /locus_tag="ACHOQQ_RS13655"
                     /old_locus_tag="pgaptmp_002731"
     CDS             complement(7060..7737)
                     /gene="kdpE"
                     /locus_tag="ACHOQQ_RS13655"
                     /old_locus_tag="pgaptmp_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGU4470960.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(7734..10418)
                     /gene="kdpD"
                     /locus_tag="ACHOQQ_RS13660"
                     /old_locus_tag="pgaptmp_002732"
     CDS             complement(7734..10418)
                     /gene="kdpD"
                     /locus_tag="ACHOQQ_RS13660"
                     /old_locus_tag="pgaptmp_002732"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGU4470961.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLLVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            complement(10411..10983)
                     /gene="kdpC"
                     /locus_tag="ACHOQQ_RS13665"
                     /old_locus_tag="pgaptmp_002733"
     CDS             complement(10411..10983)
                     /gene="kdpC"
                     /locus_tag="ACHOQQ_RS13665"
                     /old_locus_tag="pgaptmp_002733"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGU4470962.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            complement(10992..13040)
                     /gene="kdpB"
                     /locus_tag="ACHOQQ_RS13670"
                     /old_locus_tag="pgaptmp_002734"
     CDS             complement(10992..13040)
                     /gene="kdpB"
                     /locus_tag="ACHOQQ_RS13670"
                     /old_locus_tag="pgaptmp_002734"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGU4470963.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTCILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            complement(13063..14736)
                     /gene="kdpA"
                     /locus_tag="ACHOQQ_RS13675"
                     /old_locus_tag="pgaptmp_002735"
     CDS             complement(13063..14736)
                     /gene="kdpA"
                     /locus_tag="ACHOQQ_RS13675"
                     /old_locus_tag="pgaptmp_002735"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGU4470964.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPFGSGLARLINDIPLPGTAGVER
                     VLFSALGVSDREMNWKQYLSAILCLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFSLIRAFTRQSM
                     STLGNAWVDLLRITLWVLAPVALLIALFFIQQGALQNFQPYQAVTTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            complement(14736..14825)
                     /gene="kdpF"
                     /locus_tag="ACHOQQ_RS13680"
                     /old_locus_tag="pgaptmp_002736"
     CDS             complement(14736..14825)
                     /gene="kdpF"
                     /locus_tag="ACHOQQ_RS13680"
                     /old_locus_tag="pgaptmp_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGU4470965.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
BASE COUNT         3644 a         4105 c         3903 g         3305 t
ORIGIN      
        1 acgtcaaaga ggattaaccc atgcgtacgt ttagtggcaa acgtagtacg ctggcgctgg
       61 ctatcgccgg tgttacagca atgtcgggct ttatggcaat gccggaggct cgcgccgaag
      121 gattcatcga cgattcaacc ttaaccggcg gtatctatta ctggcagcgt gaacgcgacc
      181 gtaaagatgt taccgacggc gacaaataca aaaccaacct ttctcactcc acctggaatg
      241 ccaacctcga ttttcagtct ggttatgctg ctgatatgtt cggccttgat attgccgcgt
      301 ttacggcgat tgaaatggcg gaaaacggcg acagctctca cccgaacgaa atcgcgtttt
      361 caaaaagtaa taaagcctat gacgaagact ggtccggcga taaaagcggt ataagcctgt
      421 ataaagcagc ggccaaattt aaatacggtc cggtttgggc gagggcaggt tatattcagc
      481 caaccggtca gacgctgtta gcgccgcact ggagctttat gccaggtacc tatcagggtg
      541 cggaagccgg agcgaatttt gattacggcg atgccggtgc gttgagtttc tcctacatgt
      601 ggaccaacga atacaaagca ccgtggcatc tggaaatgga tgagttttat cagaacgata
      661 aaactaccaa agttgattat ctgcactcca tcggtgcgaa atacgacttc aaaaataact
      721 tcgtactgga agcggcattt ggtcaggcgg aagggtatat cgatcaatac tttgccaaag
      781 ccagctacaa atttgatatc gccggtagcc cgttaaccac cagctaccag ttctacggta
      841 cgcgcgataa agttgacgat cgcagcgtca acgatcttta tgacggcacc gcctggctgc
      901 aagcgttgac ctttggttac cgggcggctg acgtagtgga tttgcgcctc gaaggcacct
      961 gggttaaagc tgacggtcag cagggatact tcctgcaacg tatgactcca acctacgctt
     1021 cctcgaacgg tcgcctggat atctggtggg ataaccgttc tgacttcaac gccaacggcg
     1081 aaaaagcggt cttcttcggt gcgatgtatg acctgaaaaa ctggaatctt ccaggcttcg
     1141 ccatcggcgc ttcctacgtt tacgcatggg atgctaaacc tgcgacctgg cagagcaatc
     1201 cggatgcgta ctacgacaaa aaccggacta ttgaagagtc tgcctacagc ctggatgcgg
     1261 tctacaccat tcaggacggt cgcgccaaag gcacgatgtt caaactgcac ttcaccgaat
     1321 acgacaacca ctccgacatc ccaagctggg gcggcggtta cggtaacatc ttccaggatg
     1381 agcgtgacgt gaaatttatg gtaatcgcac cattcaccat cttctgatgc ccgacgcggc
     1441 aggttttgtg cctgccgcac gtttgaggaa tttgctatga aaaaactgat tctcatcgcc
     1501 atgatggcat cggggctggt agcttgtgcg caatcaaccg caccacagga agacagccgt
     1561 ctgaaagagg catacagcgc ctgtatcaac accgcacaag gttcgccgga aaaaattgaa
     1621 gcctgccaga gcgtgttaaa cgtgctgaag aaagagaaac aacatcagca gtttgccgac
     1681 caggaaagtg tccgcgtact ggattatcag cagtgtctac gtgcaacgca aaccggaaat
     1741 gatcaggcgg tgaaagccga ttgtgataag gtctggcagg aaattcgcag taataacaaa
     1801 taagtgagag ctgtaactct cgcttttctt atttcccttg cataaaaaag ccaacccgca
     1861 ggttggcttt tctcgttcag actggcttat ttgccttcgt gcgcgtgctc atcttcgcgg
     1921 caatcgcctt cggcacagtg accgtaaaga tagagactgt ggttagtcag gcgaatgcca
     1981 tgttttgcgg caatttcacg ctgacgcgct tcgattgaat catcactaaa ttcgataacc
     2041 ttgccgcagt cgaggcagat caggtgatcg tggtgatgtt gctgtgtcag ttcaaatacg
     2101 gatttaccgc cttcaaaatt gtggcgggtg acgataccag cgtcgtcaaa ctggttcagt
     2161 acgcgatata ccgtagccag accaatttct tcacccatat cgatcagacg tttgtataaa
     2221 tcttccgcac tgacgtgatg gttgtccggc tcctgaagaa cttccaggat ttttaaacgt
     2281 ggaagcgtta ctttcaggcc agctttcttt agggcggtat tgttatcagt catgcggaat
     2341 ctgtcctgtt actaagcggt tcacttcatt agaagaagtg acagaatttg ctcttgagat
     2401 aatgcgtatc attatagaat tgccacgcct aaatgaaaac cacaagtccc tggcaaatat
     2461 tgttaataaa aacgtggcac agcttacatt tacaacggca aggccacttg aacaccggga
     2521 cattgtacag gtacaacagc aaaagttaca aatttgtagc aattattttg attggcatta
     2581 tctattaata cggcgtagac atgagtctac gccgcatcac atcaggcatt gagaatttcg
     2641 tcgagatgca actcttcaga aatctgttta acccattttt ctacacgttc agcggtcagt
     2701 tccggctgac ggtcttcgtc gatagccaga ccgacaaagt ggtcgtcatc tgccagacct
     2761 tttgatgctt cgaaatgata gcccgcagtt ggccagtgac caacgatggt tgcaccgcgc
     2821 ggttcaatga tgtcgcggat ggtgcccaat gcgtcgcaga aatattcggc gtaatcttcc
     2881 tggtcaccac aaccaaacag cgcaaccagt ttgccattga aatcaatctc ttcgagagtc
     2941 gggaagaagt catcccagtc acactgcgct tcgccgtaat accaggttgg gatgcccagc
     3001 agcagaatgt cataagcttc cagatcttct ttgctgcttt ttgcaatgtc atggacatcg
     3061 gcaacgtctt taccaagctg tttttgaatc atttttgcga tattttcggt attaccggtg
     3121 tcgctgccga aaaagatgcc agtgatagcc atgagtgaaa taacctcttg aaacttattg
     3181 aaatgggggt ggaaaattgc ccacggataa aggcaatcat agcagaacag gcagtcttgc
     3241 ggaatcagca aacgagcagg actgcacact gtgctacatg aaagtggaaa tttaaacgat
     3301 gccctgacta cgcagcgccg ccagttgctg cattaacatc tcttcgatca gttcgctacg
     3361 gctcatattg cgcgattccg ccagttcgtt cagcgcctcg acagcttccg cgttcagctt
     3421 cagttcgaca cgcttaaggc cacgtacttt gtcgcgtttt agttggttgc gtttattaat
     3481 acgcagctgt tcatcgcgcg aaagcggatt agttttcggt cgtcccggtc gacgctcgtg
     3541 cgcgaacaga tctaatgtcg tacggtccgt ttgttctttg gccatgatct tggtgacttc
     3601 gggggaaaca atcagccagg cgcctgcccg gatggatagc gcgccataat acaacagcga
     3661 gaggagtctc gccaacgccc acgcgcggaa agcgacgcgg acgctgggtt tttaatcagt
     3721 tgcgttaatc attgagatag cgacggatag cgcgtaatac cgcatctggt ttttcagcat
     3781 ggacccagtg acccgcgcct gcaatcacat gcgcccgtgc ctgtggaaat tgtgccagta
     3841 aatcatcacg gtactgctcg ctaacatacg gagaattgcc gccagggata aacagggcag
     3901 ggtgatccca tgccgggatt ttctcccaac ccacaatatg cggatactga tcccacaata
     3961 ccggcacgtt aaagcgccac tccccgtcaa caaaagattt caacagaaac tgaatcaccc
     4021 cttcttcatt aagatgctgg cgcattattg ctgctgcttg ctggcgagtt tgtgcgtccg
     4081 attcactgac cgcgttgata gccgagaaaa tctcatcatg acggcgtacg tgatagtcga
     4141 ccggtgcgat atcgatcgcc accagtttat cgatgcgatc gggggccagt gcggtaagtg
     4201 ccattaccgc tttaccgccc atggagtgac cgataaatgt tgctttgtcg atctgcagtg
     4261 catccagggt atcaacaaga tcctgcgcca tcgtcgggta attcattacc ggatctctcg
     4321 gtgaaagacc gtggttacgc atatcaacct ggatgatatt gtgatcgttt accagatcgc
     4381 gagccagtac gccaaggttg tcgaggctgc caaacagacc atggacaaga acgatgggag
     4441 aattattgtg ctggttttgt gcagtttgcg cgcggatatt caatttcatg gcaaagttct
     4501 ttttttcgcg ttgtcgggtt agggtattat gttgaccatt gtgccacagg gctgcaacaa
     4561 ataaggttta ttccgagttt ttctgcaagc caggcttgac gctatccgct gccgggattt
     4621 attcatatac tcctggcgac ttgtattcag ctaagacact gcactggatt aagatgaaaa
     4681 cgattgaagt tgatgatgaa ctctacagct atattgccag ccacactaag catatcggcg
     4741 agagcgcatc cgacatttta cggcgtatgt tgaaattttc cgccgcatca cagcctgctg
     4801 ctccggtgac gaaagaggtt cgcgttgcgt cacctgctat cgtcgaagcg aagccggtca
     4861 aaacgattaa agacaaggtt cgcgcaatgc gtgaacttct gctttcggat gaatacgcag
     4921 agcaaaagcg agcggtcaat cgctttatgc tgctgttgtc tacactatat tctcttgacg
     4981 cccaggcgtt tgccgaagca acggaatcgt tgcacggtcg tacacgcgtt tactttgcgg
     5041 cagatgaaca aacgctgctg aaaaatggta atcagaccaa gccgaaacat gtgccaggca
     5101 cgccgtattg ggtgatcacc aacaccaaca ccggccgtaa atgcagcatg atcgaacaca
     5161 tcatgcagtc gatgcaattc ccggcggaat tgattgagaa ggtttgcgga actatctaaa
     5221 acgttgcaga caaaggacaa agcaatggca atccacaatc gtgcaggcca acctgcacaa
     5281 cagagtgatt tgattaacgt cgcccaactg acggcgcaat attatgtact gaaaccagaa
     5341 gcagggaatg cggagcacgc ggtgaaattc ggtacttccg gtcaccgtgg cagtgcagcg
     5401 cgccacagct ttaacgagcc gcacattctg gcgatcgctc aggcaattgc tgaagaacgt
     5461 gcgaaaaacg gcatcactgg cccttgctat gtgggtaaag atactcacgc cctgtccgaa
     5521 cctgcattta tttccgtact ggaagtgctg gcagcgaacg gcgtagatgt tattgtgcag
     5581 gaaaacaatg gcttcacccc gacgcctgcc gtttccaatg ccatcctggt tcacaataaa
     5641 aaaggtggcc cgctggcaga cggtatcgtg attacaccgt cccataaccc gccggaagat
     5701 ggtggtatca agtacaatcc gccaaatggt ggcccggcag ataccaacgt cactaaagtg
     5761 gtggaagaca gagccaacgc actgatggcc gatggcctga aaggcgtgaa gcgtatctcc
     5821 ctcgacgaag cgatggcatc cggtcatgtg aaagagcagg atctggtgca gccgtttgtg
     5881 gaagggctgg ccgatatcgt tgatatggcg gcgattcaga aagcgggcct gacgcttggc
     5941 gttgatccgc tgggcggttc cggtatcgaa tactggaagc gtattggcga gtattacaac
     6001 ctcaacctga ctatcgttaa cgatcaggtc gatcaaacct tccgctttat gcaccttgat
     6061 aaagacggcg cgatccgtat ggactgctcc tccgagtgtg cgatggcggg cctgctggca
     6121 ctgcgtgata agttcgatct ggcgtttgct aacgacccgg attatgaccg tcacggtatc
     6181 gtcactccgg caggtttgat gaatccgaac cactacctgg cggtggcaat caactacctg
     6241 ttccagcatc gtccgcagtg gggcaaagat gttgccgttg gtaaaacgct ggtttcttct
     6301 gcgatgatcg accgtgtggt caatgacttg ggtcgtaagc tggtagaagt cccggtaggt
     6361 ttcaaatggt ttgttgatgg tctgttcgac ggcagcttcg gctttggcgg cgaagagagc
     6421 gcaggggctt ccttcctgcg tttcgacggc acgccgtggt ccaccgacaa agacggcatc
     6481 atcatgtgtc tgctggcggc ggaaatcacc gctgtcaccg gtaagaaccc gcaggaacac
     6541 tacaacgaac tggcaaaacg ctttggtgcg ccgagctaca accgtttgca ggcagctgcg
     6601 acttccgcac aaaaagcggc gctgtctaag ctgtctccgg aaatggtgag cgccagcacc
     6661 ctggcaggtg acccgatcac cgcgcgcctg actgctgctc cgggcaacgg tgcttctatt
     6721 ggcggtctga aagtgatgac tgacaacggc tggttcgccg cgcgtccgtc aggcacggaa
     6781 gacgcatata agatctactg cgaaagcttc ctcggtgaag aacatcgcaa gcagattgag
     6841 aaagaagcgg ttgagattgt tagcgaagtt ctgaaaaacg cgtaaacaca ttaaataaga
     6901 aaaagggcgg tcggaagatc gccctttttt acgtatgaca aacacagaat tgcctgatgc
     6961 gctacgctta tcaggcctac gaggatggtg caatatattg aatttacgcg attttgtagg
     7021 ccggataagg cgttcatgcc ggatccagca tttaaccgcc taaagcataa accgataacc
     7081 aataccggtt tcagtaatga aatggcgggg gcgagcggga tcctgttcca gtttttgtcg
     7141 cagatgcccc atataaatcc gcaaataatg actgtgttcg accgcgtttg gtccccatac
     7201 ctggttaagg agctggcgct gggtgagcac ttttccagca ttgttgagca gcaccgccag
     7261 caggcggaat tcaatcggcg tgagatgcac ctcttcctca ccccggtgaa tcacgcgggc
     7321 ggctaaatcg acggtaacat cggaaaattt taccagcgga tcgggcgcgg cggtggcgga
     7381 gtggcggcgt aatgcgacgc gcagacgggc ctgcaattcg ccaatgccaa acggcttact
     7441 cagataatca tctgctccgg catccagcgc ggcgattttg tcgctctctt cgctacgtgc
     7501 ggaaagcaca atcaccggca ccgcgctcca ctggcgcagg tcgcggataa actcaatccc
     7561 atcaccatcg ggcaggccga gatcgagaat aatcaaatct ggcttacggg tagccgcttc
     7621 cagcaagccg cgttgcagcg tttcggcctc atagacgcgc atcccgtcgc cctccagcgc
     7681 cgtgcgcaga aagcgacgaa tagcctgttc atcttcaaca atcagaacgt ttgtcacata
     7741 tcctcatgaa attcttcaag ttcaggggca gtttgctggg gaagtgtaac acgaaaacag
     7801 gcaccacctt ccggtcggtt gaacgcggta atagtgcccc cgtgtacatc cactatcgcc
     7861 cgacaaattg ccagtccaag ccctaccccc ggtactgccg actctttatt cccgcgagca
     7921 aacttatcaa atatcgtctg ctcctggcct ggcggaagac cggggccgtt atcccagaca
     7981 tccagttgta gattttcgcc ctcaacgtgg gcatcgatac caatttcggc ctgcgcaccc
     8041 gcatatttca ccgcgttctc cagcagatta atcagcaccc gttcaaagag tggcccgtca
     8101 acgtggatta aggtcagcgg ttctggcaga gaaagattga tgggcgacga taaacccggt
     8161 tccagcattt gcagcgcgct gccgactact tcttccagcg ttaaccactc tttcttcaaa
     8221 ttaaagccgc cggactgaat tcgcgccata tccagtagat tattcaccag tcgggtagtg
     8281 ttcagcacat gctgacgaat ctcgctggcc tggcgggcgt ggggtgatcc ttcgcttgcc
     8341 agatcgagcg ttaagatttc tgcctgacca aacagcaccg taagcggagt gcgtaaatca
     8401 tgcgaaagcg ccgccagcag ggcgttgcgg atctgttcac gttcgcttgc catccgcgcc
     8461 tgttcttcgc tggcggtcag cgtcagccgc tcaagggcat tggcgactaa cagcgtaaac
     8521 gtctccagca ggcgctgctg ttccgggatc atcaactgac gcagattccc cggttccacc
     8581 accaacaacc cgtaggtttt ctcaccgctt tttaacggca aaatctggta cggcacgccg
     8641 ggtaacgtgt cggtgcccgc acccgcaggc aggcctttat caaaactcca ctgcgcgatg
     8701 gcatcgtccc acggcgtcat tccttgcgga tgtgttaacg gctgcaattt accgttgtca
     8761 tcgggcaaca acacctgact gcgggcatga aacgtggagg caataaattg ttcgctggtg
     8821 gcggcgatgt cctgcggact gcggcccacc gccagagctt tcgacatttc atataagtgc
     8881 cgtgtgcgtt gctcgcggta acgggctacc cgtgcctgat aacgcacacc cgcagtaagg
     8941 ttcccgatca ccagcccgac ggttaacatc accgcgaagg tcagcagata ttgcacatca
     9001 gagacggcga gcgtgccgcg tggggcgata aaaaagagat cgaaactcac cacattaatg
     9061 acggtagcaa ccaccgaagg ccagcgtcca taaaatagcg ccaccaccac cacgccaagt
     9121 agatacagca tcaccaggtt ggcggcatca aacgccatca gccactgcat ggcaattaag
     9181 gtgataacgg cgcataacgc ggcggcaacc acgcatccct gaatctgtac ccgccacttg
     9241 tctttaaaag agcggctatc cggcgcgttg ttaatcgtgc gggcgggtgg ttcatcaagc
     9301 gcgaccagca cctgatcgag atcgggggcg atgcgcgcca gtcggtcagc aaacgtttcc
     9361 cgacgccacc agcggcgcga ggctgggcga ccgagaataa tcttgccgag attatgttca
     9421 cgggcataac gcactaccgc tttctcttcc gctggatcag aaagtgttgc cgtctccgcg
     9481 cccagctcct gcgccagacg taaggcgctg agaattgccc gacgtttttt ctccggtaag
     9541 cgatgcaggg caggggtttc aacatacacc gcgtgccaga cgctccccag ccgtgacgcc
     9601 agccgcgccg ccgcgcggac cagtttttcg ctgccggtgt tatggccgat gcataaaagg
     9661 atcgcgtcgc gcgtgtgcca gactttctct tcgccaggat gcccccgcca ggcgcgcatt
     9721 tgctcatcaa cgcgatcggc agtacggcgc agtgccagtt cgcgcagggc gatcagattg
     9781 cctttgcgga aaaaatgttc aatggcgcgc tccgcctgcc cggcaatata gactttgcct
     9841 tcttttagcc gctggcgcag atcgtccggg ggcaagtcca ccagcaccac gtcgtcggcg
     9901 gcatcgaaaa aaggatcggg cacggtttcc cgtacctgaa ttccggtgac gccgctgacc
     9961 acatcattca gactttccag atgctgaacg ttgacggttg tgaaaacatc aatgccagct
    10021 tccagcagtt cttcgatatc ctgccagcgt ttgggatgac gggaacctgg cgcattactg
    10081 tgcgccagtt cgtccattaa gatcagcgcc ggacggcggg cgagggcggc atcgagatca
    10141 aactcgctga tatgccgccc acggtacgcc tggcgtttca gcggcagaac agccagccct
    10201 tccagcattg cggcggtatc tttccgcccg tgggtttcta ccacgccaac cacaatatcc
    10261 agcccttgcg cccgcagtcg ctgggcttct gccagcatcg cccaggtctt cccgacgcct
    10321 gcgcaggcac cgaaaaaaac tttcagcttc ccccgatgcg gcgcggcagt ttgttccagc
    10381 agacgatcgg ggtcgggacg taagggttcg ttattcatca agtttatcca gcgccagatt
    10441 gagttcgaca atgttgacga ccggctggcc gatatatttc accagcggtt gttggctgta
    10501 ttttgcgatc agttgcgtga gctgttcaac gctgagatta cgcgctttcg ctacgcgtgg
    10561 gatctgccag gccgccgctt gcggggtgat gttattgtcc agcccgcttg ccgacgccgt
    10621 caccagttca accggaacgc tcgtgctggc attcgggtta gcggcccgta atgcagcaac
    10681 gcgtgcggct attagtttat ccagctcagg gttactgacc gccagattgc tcccgccaga
    10741 agcctgtgga ttataaggca tttctgccgt tgccgacggg cgaccatgaa aatagccgtt
    10801 gccggtaaaa ttctgcccga ttaatgccga accccgcacc gtatcacctt cacggatcaa
    10861 cgaaccgttg gcctgccagg gaaaccacca ttgccccagc gcggtggtca gcagcgggta
    10921 aacaccgcca gtaatcaata acagaaagat aaatgtcgat aatgccggac gtaatccacg
    10981 catggtaaac ctcacaccag accgcaaacg gtcagtagta aatcaatgac tttgatacca
    11041 ataaacggca ccagcagccc acccagaccg taaatccata agttacggcg caacatggca
    11101 gaagcggtaa gcggtttata actcacgcct tttaacgcca ggggaatcaa aaagacgata
    11161 atcaaggcgt tgaaaatgac cgcactgaga atggcggagt cgggcgaatg caggcgcatg
    11221 atgttcagcg catttaactg tggatacgtt accgcgaatg ccgccggaat aatggcgaag
    11281 tatttcgcca catcgttggc aatgctgaag gtggtcagcg agccacgggt catcagcatc
    11341 tgtttgccaa tatgtaccac ctcgatcaac ttggtcgggt tagagtcgag atccaccata
    11401 ttgcccgctt ctttcgccgc ctgagtaccg gagttcatcg ccaccgcgac atctgcctgc
    11461 gccagcgccg gagcatcgtt ggtgccgtcg ccggtcattg ctaccaaacg gccttccgcc
    11521 tgatactgac ggatcaatgc cagcttggcc tccggtgtcg cttcggcgag aaaatcatcg
    11581 acgcccgctt ccgcggcaat ggcggcggca gtcagacggt tatcgccggt aatcatcacc
    11641 gttttaatac ccattttgcg cagttgggcg aagcgttctt taatgccgcc tttgacgata
    11701 tctttcaacg caataacgcc cagcacacga gaaccttcta ctactaccag cggtgtggcc
    11761 ccctgacgcg caacctgatc gactttttga tcaacatcgg cagggaagtg accaccgtta
    11821 gcctcaacat ggcggcgaat ggcatcgaca gaacctttac ggatcatgcg gttgtcgata
    11881 ttgatcccgc tcatccggct ttgcgcagta aacggtacaa aggtggcatg gagcgactgc
    11941 acatcgcgct cgcgcaggtt aaaacgctgc ttggcgagga tcacaatact gcggccttcc
    12001 ggcgtttcat cagccagcga agccagttgt gcggcgtcag ccagcgtttt ttcatccacg
    12061 ccctgcgcag ggataaactc cgatgcctga cggttaccga gtgtgatggt gccggtttta
    12121 tccagcagca gaacgtcaac gtcacctgcc gcttcaactg cacgtccgct ggtggcaatc
    12181 acattcgcgc ccagcatccg actcatcccg gcgacgccga tcgctgacaa caggccgcca
    12241 atagtggttg ggatcagaca gaccagcagc gccaccagta ccgttacgct gactgcatta
    12301 ccgccccacg cggaaaacgg ccacagcgtg gcggttgcca gtaaaaagac gatagtcagg
    12361 gcaatcagca ggatggtcag ggcaatctcg ttcggcgttt tgcgtcgctg tgcgccttcc
    12421 accatcgcga tcatccgatc cagaaatgtc tcgccggggt taacgctaca ctcaatcacc
    12481 agccagtcag aaagaataca cgtgccgccg gtgacggagg caaaatcgcc gccggattca
    12541 cggatcaccg gtgccgattc cccggtgatg gcgctttcat cgaccgatgc gccgccttca
    12601 ataacttcac catcgcaggg gataatatcg cctgcttcta ccagtacgat atcgccttta
    12661 cgcagttgat cggcgggaac tttgtccgcc gcagcgccat atttcggttc gcgcagcttg
    12721 cgggcaaagg ccgttttttt cacccctttc aggctgttgg cctgcgcttt actgcggcct
    12781 tctgccagcg cctcggcgaa attagcgaac agtacggtga cccacagcca gccgctaatg
    12841 gccacgctaa acagcgcatt gccaggcatc gcgccgcttg ccatcgcgat gctaatacag
    12901 gtggtcagca gactgccgat ccagacgata aacatcaccg gattgcgcca ttgcgcctgc
    12961 gggtttaatt ttttcaccgc ttctttcagc gcctgaacga caagtgttgg ttcgaatagc
    13021 gccagttgtt tacgactcat attcagtgct cactcaatat catcaggaga gatattccgc
    13081 caccggacca agcgccaggg cagggataaa ggtcagtgcg ccaaccagca acacggtgcc
    13141 gattaacagg ccaacaaaca gcgggccgtg cgttggcagc gtgccggagc tggcgggttg
    13201 gctcttttta ctcaccagcg aaccggcaat tgccatcacc gggataatca ccccgaagcg
    13261 accgacaaac atgcagaacg ccagtaaaca gttccagaac ggagagttgg cgcttaatcc
    13321 ggcaaaggcg ctgccgttgt tgttagcggc ggatgacacg gcgtacagca cttcgctaaa
    13381 accatgcggg ccagggttga gcatggcgct acgtccggcg tcggtcatca ttgccagcgc
    13441 cgcgcccatc agcaccagcg tcggggtgac cagaatcgcc agtgcagtca gtttcatctc
    13501 acgtacgtcg atttttttac ccagatattc cggtgtacga ccaatcatca gcccggcaat
    13561 aaacaccgcc agcaggacaa acagcatcat gccgtaaaga ccagaaccga caccgccgaa
    13621 taccacttca ccaatttgca tcagccacat cggcaccatg ccaccgagag cggtaaacga
    13681 atcatgcatc gcaatcaccg cgccacagga cgccgccgta gtcacgaccg caaacaggct
    13741 actgaccagc acgccgaaac ggctctcttt accttccata ttgatgctgc tgtctgcgcc
    13801 gagcgccagc agatgaggat taccctgaac ttctgcccac atcaccacgc ctacgcagat
    13861 gacaaaaatc actgacatcg cccacagcaa catgcgcccc tggcggcgat cgcccgtcac
    13921 ttcaccaaag gcaaagcaca gcgctgttgg gatcaagaag atcgccagca tctgcacgaa
    13981 gttggtcagt gcggttgggt tttcaaacgg atgcgacgag ttggcattaa agaagccacc
    14041 gccgttagtg ccgagcatct tgatcgcttc ctgagaagcc actggcccca ttggtaacag
    14101 ctgctgcgct ccttcaacgg tcgtcacagc ctgataaggc tgaaaatttt gcagtgcacc
    14161 ttgttgaata aaaaacagcg cgatcaacaa cgccacaggg gccaacaccc ataacgtgat
    14221 gcgtagcaga tcgacccagg cattcccgag tgtgctcatg ctctggcggg taaatgcacg
    14281 gatgagggaa aaaatcaccg caatcccgct ggcggcagaa agaaagtttt gcaccgttaa
    14341 gcccgccatc tggctgaaat agctcaacgt ggtttcaccg ctataagatt gccagttggt
    14401 attagtgaca aagctgacgg cagtattcag cgccagatcc cacgacagcc ccggcaactg
    14461 ctgtggatta agcggcagat agtgctgacc gagcagcatg aaaaaaagca ccgccagccc
    14521 cagcatgttc agacagagaa tggctgagag atactgcttc cagttcatct cacggtcaga
    14581 gacgccaagc gcgctaaaga gtacgcgttc aacgcccgct gtaccgggaa gaggaatatc
    14641 attaatcaac cgtgccagcc cgctgccgaa aggacgcgcc agcaccatta ataccagtaa
    14701 aaacgtggca atcagtaaga acccttgcgc agccatcaga acgcctccgc attgatcagg
    14761 gcataaacca gataacccag taataaaaac accagcaata cgccggttat cacgcctgca
    14821 ctcacagtgc acctccagtg gcctaaaagt gataccggaa gggtagaatt ctggctgcaa
    14881 agatttcgca aaaatctgcg gacggggtgt aaaaaaagta taaaaatggc aaaagccatg
    14941 atttaactaa tgtttag
//
LOCUS       DAZKFU020000112        14814 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZKFU020000112 DAZKFU020000000
VERSION     DAZKFU020000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.113"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            333..1280
                     /gene="cyoA"
                     /locus_tag="ACHOQQ_RS13685"
                     /old_locus_tag="pgaptmp_002737"
     CDS             333..1280
                     /gene="cyoA"
                     /locus_tag="ACHOQQ_RS13685"
                     /old_locus_tag="pgaptmp_002737"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGU4470966.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANDPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1302..3293
                     /gene="cyoB"
                     /locus_tag="ACHOQQ_RS13690"
                     /old_locus_tag="pgaptmp_002738"
     CDS             1302..3293
                     /gene="cyoB"
                     /locus_tag="ACHOQQ_RS13690"
                     /old_locus_tag="pgaptmp_002738"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGU4470967.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3283..3897
                     /locus_tag="ACHOQQ_RS13695"
                     /old_locus_tag="pgaptmp_002739"
     CDS             3283..3897
                     /locus_tag="ACHOQQ_RS13695"
                     /old_locus_tag="pgaptmp_002739"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGU4470968.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMAPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3897..4226
                     /locus_tag="ACHOQQ_RS13700"
                     /old_locus_tag="pgaptmp_002740"
     CDS             3897..4226
                     /locus_tag="ACHOQQ_RS13700"
                     /old_locus_tag="pgaptmp_002740"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGU4470969.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4238..5128
                     /gene="cyoE"
                     /locus_tag="ACHOQQ_RS13705"
                     /old_locus_tag="pgaptmp_002741"
     CDS             4238..5128
                     /gene="cyoE"
                     /locus_tag="ACHOQQ_RS13705"
                     /old_locus_tag="pgaptmp_002741"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGU4470970.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5277..6641
                     /locus_tag="ACHOQQ_RS13710"
                     /old_locus_tag="pgaptmp_002742"
     CDS             5277..6641
                     /locus_tag="ACHOQQ_RS13710"
                     /old_locus_tag="pgaptmp_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4470971.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCIGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6769..7260)
                     /gene="yajQ"
                     /locus_tag="ACHOQQ_RS13715"
                     /old_locus_tag="pgaptmp_002743"
     CDS             complement(6769..7260)
                     /gene="yajQ"
                     /locus_tag="ACHOQQ_RS13715"
                     /old_locus_tag="pgaptmp_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGU4470972.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7428..8339
                     /gene="panE"
                     /locus_tag="ACHOQQ_RS13720"
                     /old_locus_tag="pgaptmp_002744"
     CDS             7428..8339
                     /gene="panE"
                     /locus_tag="ACHOQQ_RS13720"
                     /old_locus_tag="pgaptmp_002744"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGU4470973.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8302..8892
                     /gene="yajL"
                     /locus_tag="ACHOQQ_RS13725"
                     /old_locus_tag="pgaptmp_002745"
     CDS             8302..8892
                     /gene="yajL"
                     /locus_tag="ACHOQQ_RS13725"
                     /old_locus_tag="pgaptmp_002745"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGU4470974.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8946..10394)
                     /gene="thiI"
                     /locus_tag="ACHOQQ_RS13730"
                     /old_locus_tag="pgaptmp_002746"
     CDS             complement(8946..10394)
                     /gene="thiI"
                     /locus_tag="ACHOQQ_RS13730"
                     /old_locus_tag="pgaptmp_002746"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGU4470975.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGVDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10600..10842
                     /gene="xseB"
                     /locus_tag="ACHOQQ_RS13735"
                     /old_locus_tag="pgaptmp_002747"
     CDS             10600..10842
                     /gene="xseB"
                     /locus_tag="ACHOQQ_RS13735"
                     /old_locus_tag="pgaptmp_002747"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGU4470976.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10842..11741
                     /gene="ispA"
                     /locus_tag="ACHOQQ_RS13740"
                     /old_locus_tag="pgaptmp_002748"
     CDS             10842..11741
                     /gene="ispA"
                     /locus_tag="ACHOQQ_RS13740"
                     /old_locus_tag="pgaptmp_002748"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGU4470977.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            11766..13628
                     /gene="dxs"
                     /locus_tag="ACHOQQ_RS13745"
                     /old_locus_tag="pgaptmp_002749"
     CDS             11766..13628
                     /gene="dxs"
                     /locus_tag="ACHOQQ_RS13745"
                     /old_locus_tag="pgaptmp_002749"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGU4470978.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRLMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            13683..14657
                     /gene="yajO"
                     /locus_tag="ACHOQQ_RS13750"
                     /old_locus_tag="pgaptmp_002750"
     CDS             13683..14657
                     /gene="yajO"
                     /locus_tag="ACHOQQ_RS13750"
                     /old_locus_tag="pgaptmp_002750"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGU4470979.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEDLGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
BASE COUNT         3402 a         3807 c         3906 g         3699 t
ORIGIN      
        1 ataaattcta tttttcttac acgattcagc taatgagtct ttattttctc acccccagtt
       61 gtcactccaa tgataattat ttgttaaata attgttttat ttcgtattgg ttataccaat
      121 tgcccgccca gactccttct gcactcccct tttgttataa cgcccttttg caacagcttc
      181 ttaaaatcaa cctgatatgt tttgcaacat atgtgacctg acagccaaat ccaagtaaca
      241 ggaatttaat catgtttaca gtaatgtaac cttcccgtaa aatgcccaca cactttaaac
      301 gccaccagat cccgtggaat tgaggtcgtt aaatgagact caggaaatac aataaaagtt
      361 tgggatggtt gtcattattt gcaggcactg tattgctcag tggctgtaat tctgcgctgt
      421 tagatcccaa aggacagatt ggtctggagc aacgttcact gatactgacg gcatttggcc
      481 tgatgttgat tgtcgttatt cccgcaatct tgatggctgt tggtttcgcc tggaagtacc
      541 gtgcgagcaa taaagatgct aagtacagcc cgaactggtc acactccaat aaagtggaag
      601 ctgtggtctg gacggtacct atcttaatca tcatcttcct tgcggtactg acctggaaaa
      661 ccactcacgc tcttgagcct agcaagccgc tggcacacga cgagaagccc attaccatcg
      721 aagtggtttc catggactgg aaatggttct tcatctaccc ggaacagggc attgctaccg
      781 tgaatgaaat cgctttcccg gcgaacactc cggtgtactt caaagtgacc tccaactccg
      841 tgatgaactc cttcttcatt ccgcgtctgg gtagccagat ttatgccatg gccggtatgc
      901 agactcgcct gcatctgatc gccaacgacc ccggcactta tgacggtatc tccgccagct
      961 acagcggccc gggcttctca ggcatgaagt tcaaagctat tgcaacaccg gatcgcgccg
     1021 cattcgacca gtgggtcgca aaagcgaaac agtcgccgaa ctccatgtct gacatggcag
     1081 cgttcgaaaa actggccgcg cctagcgaat acaaccaggt ggaatatttc tccaacgtga
     1141 aaccagactt gtttgccgat gtaattaaca agtttatggc tcacggtaag agcatggaca
     1201 tgacccagcc agaaggtgag cacagcgcac acgaaggtat ggaaggcatg gacatgagcc
     1261 acgcggaatc cgcccattaa aggggttgag gaagaataaa gatgttcgga aaattatcac
     1321 ttgatgcagt cccgttccat gaacctatcg tcatggttac gatcgctggc attattttgg
     1381 gaggtctggc gctcgttggc ctgatcactt acttcggtaa gtggacctac ctgtggaaag
     1441 agtggctgac ctccgtcgac cataaacgcc tcggtatcat gtatatcatc gtggcgattg
     1501 tgatgttgct gcgtggtttt gctgacgcca ttatgatgcg tagccagcag gctcttgcct
     1561 cggcgggcga agcgggcttc ctgccacctc accactacga tcagatcttt accgcgcacg
     1621 gcgtgattat gatcttcttc gtggcgatgc ctttcgttat cggtctgatg aacctggtgg
     1681 ttccgctgca gatcggcgcg cgtgacgttg cgttcccgtt cctcaacaac ttaagcttct
     1741 ggtttactgt tgttggtgtg attctggtaa acgtttctct cggcgtgggc gaatttgcgc
     1801 agaccggctg gctggcctat ccaccgctat cgggaataga gtacagtccg ggagtcggtg
     1861 tcgattactg gatatggagt ctccagctat ccggtatagg tacgacgctt accggtatca
     1921 acttcttcgt taccattctg aagatgcgcg caccgggcat gaccatgttc aagatgccag
     1981 tatttacctg ggcatcactg tgcgcgaacg tactgattat tgcttccttc ccaattctga
     2041 cggttaccgt cgcgttgttg accctggatc gctatctggg cacccatttc tttaccaacg
     2101 atatgggtgg caacatgatg atgtacatca acctgatttg ggcctggggc cacccggaag
     2161 tttacatcct gatcctgcct gttttcggtg tgttctccga aattgcggca accttctcgc
     2221 gtaaacgtct gtttggttat acctcgctgg tatgggcaac cgtctgtatc accgtgctgt
     2281 cgttcatcgt ttggctgcac cacttcttta cgatgggtgc gggcgcgaac gtaaacgcct
     2341 tctttggtat caccacaatg attatcgcca tcccgaccgg ggtgaagatc ttcaactggc
     2401 tgttcaccat gtatcagggc cgcatcgtgt tccattctgc gatgctgtgg actatcggtt
     2461 ttatcgtcac cttctcggtg ggcggtatga ctggcgtgct gctggcagta ccgggcgcgg
     2521 acttcgttct acataacagc ctgttcctga ttgcgcactt ccataacgtg atcatcggcg
     2581 gcgtggtctt cggctgcttc gcagggatga cctactggtg gcctaaagcg ttcggtttca
     2641 aactgaacga aacctggggt aaacgcgcgt tctggttctg gatcatcggc ttcttcgttg
     2701 cctttatgcc actgtacgcg ctgggcttca tgggcatgac ccgtcgtttg agccagcaga
     2761 ttgacccgca gttccacacc atgctgatga ttgcagccag cggtgcagta ctgattgcgc
     2821 taggtattct ctgcctcgtt attcagatgt acgtttctat tcgtgaccgc gaccagaacc
     2881 gtgacctgac tggcgacccg tggggtggcc gtacgctgga gtgggcaacc tcttccccac
     2941 ctccgttcta taactttgcc gttgtgccgc acgttcacga gcgtgatgca ttctgggaaa
     3001 tgaaagagaa aggcgaagcg tataaaaagc ctgaccacta tgaagaaatt catatgccga
     3061 aaaacagcgg tgcaggtatc gtcattgcag ctttctccac catcttcggt ttcgccatga
     3121 tctggcatat ctggtggctg gcgattgttg gcttcgcagg catgatcatc acctggatcg
     3181 tgaaaagctt cgacgaggac gtggattact acgtgccggt ggcagaaatc gaaaaactgg
     3241 aaaaccagca tttcgatgag attactaagg cagggctgaa aaatggcaac tgatactttg
     3301 acgcacgcga ctgcccacgc gcacgaacac gggcaccacg atgcaggcgg aaccaaaatc
     3361 ttcggatttt ggatctacct gatgagcgac tgcattctgt tctctatctt gtttgctacc
     3421 tatgccgttc tggtgaacgg caccgcaggc ggcccgacag gtaaggacat tttcgaactg
     3481 ccgttcgttc tggttgaaac tttcttgctg ttgttcagct ccatcaccta cggcatggcg
     3541 gctatcgcca tgtacaaaaa caacaaaagc caggttatct cctggctggc gttgacctgg
     3601 ttgtttggcg ccggatttat cgggatggaa atctacgaat tccatcacct gattgttaac
     3661 ggcatggctc cggatcgcag cggcttcctg tcagcgttct ttgcgctggt cggcacgcac
     3721 ggtctgcacg tcacttccgg tcttatctgg atggcggtgc tgatggtaca aatcgctcgt
     3781 cgcggcctga ccagcactaa ccgtacccgc atcatgtgcc tgagcctgtt ctggcacttc
     3841 ctggatgtgg tttggatctg tgtgttcact gttgtttatc tgatgggggc gatgtaatga
     3901 gtcattctac cgatcacagc ggcgcgtccc atggcagcgt aaaaacctac atgacaggct
     3961 ttatcctgtc gatcattctg acggtgattc cgttctggat ggtgatgaca ggggctgcct
     4021 ctccggccgt aattctggga acaatcctgg caatggcagt ggtacaggtt ctggtgcatc
     4081 tggtgtgctt cctgcacatg aataccaaat cagatgaagg ctggaacatg acggcgtttg
     4141 tcttcaccgt gctaatcatc gctatcctgg ttgtaggctc catctggatt atgtggaacc
     4201 tcaactacaa catgatgatg cactaagagc ggcggttatg atgtttaagc aatacctgca
     4261 agtaacgaaa ccaggcatca tctttggcaa cctgatctcg gtgattgggg gattcctgct
     4321 ggcctcaaag ggcagcattg attatcccct gtttatctac acgctggttg gggtgtcact
     4381 ggttgtggcg tcgggttgtg tgtttaacaa ctacatcgac agggatatcg acagaaagat
     4441 ggaaaggacg aagaatcggg tgctggttaa aggcctgatc tctcctgctg tctcgctggt
     4501 gtacgccacc ttgctgggta ttgctggctt tatgctgctg tggtttggcg cgaatccgct
     4561 ggcctgctgg ctgggggtga tgggctttgt ggtttatgtc ggcgtttata gcctgtacat
     4621 gaaacgccac tcagtctacg gcacattgat tggttcgctc tccggcgctg cgccgccggt
     4681 gattggttac tgtgcagtaa ccggcgagtt cgatagcggc gcagcgatcc tgctggctat
     4741 cttcagcctg tggcagatgc ctcactccta tgccatcgcc attttccgct ttaaggatta
     4801 ccaggcggca aacattccgg tattgccagt ggtaaaaggc atttcggtgg cgaagaatca
     4861 catcacgctg tatatcatcg cctttgccgt tgccacgctg atgctctctc ttggcggtta
     4921 cgctggatat aaatatctgg tggtcgccgc ggcggtgagc gtctggtggt taggtatggc
     4981 cctgcgcggt tataaagttg ctgatgacag aatctgggcg cgcaagctgt tcggcttctc
     5041 tatcatcgcc atcactgccc tctcggtgat gatgtccgtt gattttatgg tgccggactc
     5101 gcatacgctg ctggctgctg tgtggtaaca caacctctct attaaaaagg tgctacggca
     5161 ccttttttct tagcattaga aacatatccc tctcgaaata tttactaaaa aatccgcatg
     5221 tttaccccat tcgtttgccg ctttacacta gtcgcgaatt taaaacagag gtggtaatga
     5281 acgattataa aatgacgcca ggtgagcggc gcgcgacctg gggtttaggg accgtattct
     5341 cgttgcgcat gctgggcatg ttcatggttc tgccggttct gaccacgtac ggcatggctc
     5401 tgcaaggtgc cagcgaagca ttaatcggta ttgccattgg tatttatggt ctgactcagg
     5461 ccgtttttca gattccgttt ggcctgcttt ccgaccgcat tggtcgcaaa ccattaattg
     5521 tcggtgggct ggcagtgttt gccgccggta gcgttatcgc tgcgctctct gactccatct
     5581 ggggaattat tctgggtcgg gcgctacaag gctccggtgc gattgccgct gccgttatgg
     5641 cgctgctttc cgatctcacg cgcgaacaaa accgcaccaa agcaatggcg tttatcggcg
     5701 tgagctttgg cattaccttt gccattgcga tggtgcttgg cccgatcatc actcacaaac
     5761 ttgggctgca cgcgctgttc tggatgatcg ctattctggc aacgaccggc attgcgttga
     5821 ccatttgggt tgtgcccaac agtagcactc acgtacttaa tcgtgagtcc ggaatggtga
     5881 aaggcagttt cagtaaagtg ctggcggaac cgcggctgct gaaactcaac tttggcatta
     5941 tgtgtctgca tattttgctg atgtcgacct ttgttgccct gcccggacaa ctggctgatg
     6001 cggggttccc ggcggctgaa cactggaagg tctatctggc gacaatgcta atcgcctttg
     6061 gctcggtcgt gcctttcatt atctacgctg aagttaagcg caaaatgaag caagtctttg
     6121 tcttctgcat cgggttgatc gtggttgcgg aaattgtgtt gtggaacgcg caaacgcagt
     6181 tctggcaact ggtggtcggc gtgcagcttt tctttgtggc gtttaatttg atggaagccc
     6241 tcctgccctc acttatcagt aaagagtcgc cagcaggtta caaaggtacg gcgatgggtg
     6301 tttactccac cagccagttt cttggcgtgg cgattggcgg ttcgctgggc ggctggattg
     6361 acggcatgtt tgacggtcag ggggtatttc tcgctggcgc aatgctggcc gcagtgtggc
     6421 tggcagtcgc cagtaccatg aaagaaccgc cgtatgtcag cagtttgcgc attgaaatcc
     6481 cggcgaacat tgccgcaaac gaggcgttaa aagtgcgttt gctggaaact gaaggcatca
     6541 aagaagtgtt gattgcagaa gaagaacatt cagcttatgt gaaaatcgac agcaaagtga
     6601 cgaatcgctt tgaggttgaa caggcaattc gccaggcata aaagcgaggc gaaattgcct
     6661 gctgcactac gcttatcagg cctactaagt tcctgagtta caaagctttg taggccgaat
     6721 caggcgttca tgccgcatct gacatgaaca aagcgcaggt tgtcgcgatt aatcgcggaa
     6781 gtttttgaac tggaacggct gacccagatc gccaccgcgc accatcgcca tgacagcctg
     6841 caaatcatca cgagatttgc cagtcacgcg gatctcatcg ccctgaattt gcgcctgcac
     6901 tttcagtttg ctgtctttga tcatcttgac gattttcttc tgggtcgcgc tctcaatgcc
     6961 ctgtttcagt ttcgcttcca caaaccaggt tttaccgcta tgaacgatat tttccggtac
     7021 atccagcgaa ctgccttcaa tgccgcgctt cagcagcttg gcacgcaaaa tatccagcaa
     7081 ctgattgacc tggaaatcgg actcgctcaa cactttgatg gttttgctgg cgtcgttcag
     7141 ctcaaatgag gcttcaacgt tacggaagtc aaaacgggac tccacttcgc ggctcgcgtt
     7201 atcgaccgcg ttacgtgctt cctgaagatc aacttcagag acaatatcga aagatggcat
     7261 cttttcctct cccttcattt ttgatgcgaa gcataatacc tgcaaagtta aataaccaac
     7321 agtcctgcac caggacgtat cccttaattg tgcatagtcg caacagcatg cacaattaag
     7381 ggatagcctt attgaacacc cggagtggtt gcgggtgagg aggaacaatg aaaattaccg
     7441 tattgggatg cggtgcctta gggcaattat ggcttacagc actttgtaaa cagggtcacg
     7501 aagttcaggg ctggctgcgc gtaccgcaac cttattgtag tgtgaatctg gttgagactg
     7561 atggttcgat atttaacgaa tcgctgaccg ccaacgatcc cgattttctt gccaccagcg
     7621 atctgctcct ggtgacgctg aaagcatggc aggtttccga tgccgtcaaa agcctcgcgt
     7681 ccacactgcc tgtaactacg ccaatactgt taattcacaa cggcatgggc accatcgaag
     7741 aattgcaaaa cattcagcag ccattactga tgggcaccac cacccatgcc gcccgccgcg
     7801 acggcaatgt cattattcat gtggcaaacg gtatcacgca tattggtcca gcacggcaac
     7861 aggacggcga ttacagttat ctggcggata ttttgcaaac cgtgttgcct gacgttgcct
     7921 ggcataacaa tattcgcgcc gagctgtggc gcaagctggc agtcaactgt gtgattaatc
     7981 cactgactgc catctggaat tgcccgaacg gtgaattacg tcatcatccg caagaaatta
     8041 tgcaaatatg cgaagaagtc gcggcagtga tcgaacgcga agggcatcat acttcagcgg
     8101 aagatttgcg tgattacgtg atgcaggtga ttgatgccac agcggaaaat atctcgtcga
     8161 tgttgcagga tatccgcgcg ctgcgccaca ccgaaatcga ctatatcaat ggttttctct
     8221 tacgccgcgc ccgcgcgcat gggattgccg taccggaaaa cacccgcctg tttgaaatgg
     8281 taaaaagaaa ggagagtgaa tatgagcgca tcggcactgg tttgcctcgc ccctggtagt
     8341 gaagagactg aagccgtcac cacgatcgac ctcttggttc gcggcggtat caaagtcacc
     8401 actgccagcg tagccagcga tggcaacctg gcgattacct gctcgcgcgg cgtgaagctg
     8461 ctggcggatg cgccgctggt cgaagtggct gatggcgaat atgacgtgat cgtactgcct
     8521 ggtggcatta aaggcgcaga gtgttttcgc gatagtaccc tgctggttga aaccgttaaa
     8581 cagttccacc gttccgggcg tatcgtcgcg gctatttgcg ccgcgccagc caccgtgctg
     8641 gtcccgcacg atatcttccc gattggcaat atgaccggct tcccgacgct gaaagacaaa
     8701 attcccgccg aacaatggca ggacaagcgc gtcgtctggg atgcacgggt aaaattgctg
     8761 accagccagg ggccgggtac agctatcgac tttggtctga aaattatcga cctgttggtt
     8821 gggcgtgaaa aagcccatga agtggcatca caactggtga tggcggcagg gatttataat
     8881 tattacgagt agtgtcggat gcggtaagcg tcgcatctga ccagatgcga cgttacaacc
     8941 acaaattacg ggcgatatac cttcacattg ttaaagccct gctcgcgcag atagagcgcc
     9001 tgcagacggc tcatcacccc gcgctcacac cacaacagcc aggttttact ctggtcgaga
     9061 tcgccaaatt tggtgctcag tttatagaac ggcagagaaa ccacatcaac cccttcgact
     9121 ttcagtggct tatcttcttg ttcatcgata gaacggatat cgaggatgac gtcattcggg
     9181 ccgaagccat tgacagtttc cacttccacc acttcctgct cggtctgctg ggcgatttcg
     9241 cggatatcaa cgttattcgc ttcctcaacc actttatcga gaatgctgaa gtcgaacttc
     9301 tcttcttccg cttcaatctt cgatttaacc gctttcaccg tcgggctttt ggagatcaca
     9361 ccgcagtatt ccggcatcgt gcgagcaaag tcttcggtgc caatctggcg agccaggttg
     9421 atgatgtgct ctttgtcgta agagatcagc ggacgcagga tcagcgtatc agagacgtta
     9481 tcaatcaggc gcaggttggt cagcgtctga ctggacacct ggccgagcgc ttcgccggtg
     9541 accagcgcct gtacgccgta acgttcagcc actttagacg cggcacgcac catcatacgt
     9601 ttgaggataa cgcccatctg accgtcgtcg attttctcga gaatttcccc gacgaccggc
     9661 tcgaaattaa tagcgacaaa acgcacgcgg tgggagctgc caaagcggtt ccacagataa
     9721 tgcgccacct gacgtacgcc aatttcatgc gccgcgccgc cgaggttaaa gaagcagtaa
     9781 tgcacgcggc agccgcgacg catcaacata taactggaaa caccggagtc gaaaccaccg
     9841 gaaatgagcg acagtacatc ttcctgggtg ccgatcggga aaccaccaat accttcgtag
     9901 cggcctttaa tcagcaggag acgatcgtct tccacttcca aatggacagt cacatccgga
     9961 ttggtcagct tcacgcgcgc ggattcaata tgctgattta aaccgccgcc gacgtaacgt
    10021 tccacatcaa tcgagctaaa atcatgtttg ccacggcgct tcacgcgtac gcagaaggtt
    10081 ttgccttcca gctgatcgcg atactgaacc aacgctttct cgaaaatatc gtgcatgtcg
    10141 gtaaacggca catcttcgac ttcgagaata tggtggatac ccggaatacg ggtcagagcg
    10201 tcgcgaatag ccagacgctg gttttcatct tttgcgcgaa cttcgatgtt atcccagtgg
    10261 cggacgacag cgagcgtctc atcatagtgc tttaaaacgt tacgaatgtt cccggtaagg
    10321 atttttataa agcgcaagcg cacagattgg cttttgatgg tgatttccgg gaacaattta
    10381 atgataaact tcatggcggc aatggttcgt tggcaagcct taagcgactt gtatagggaa
    10441 aaatacagca gcccacacct gcggctgcat ccaggcgcgg aagtatacca ctaacatcgc
    10501 tttgctgcgc acatcacctt accattgcgc gttatttgct atttgccctg agtccgttac
    10561 catgacgggg cgaaaaatat tgagagtcag acattcatta tgccgaagaa aaatgaggcg
    10621 cccgccagct ttgaaaaggc gctgagcgag ctggaacaga ttgtaacccg tctggaaagt
    10681 ggcgacctgc cgctggaaga ggcgctgaac gagttcgaac gcggcgtgca gctggcacgt
    10741 caggggcagg ccaaattaca acaagccgaa cagcgcgtac aaattctgct gtctgacaat
    10801 gaagacgcct ctctaacccc ttttacaccg gacaatgagt aatggacttt ccgcagcaac
    10861 tcgaagcctg cgttaagcag gccaaccagg cgctgagccg ttttatcgcc ccactgccct
    10921 ttcagaacac tcccgtggtc gaaaccatgc agtatggcgc attattaggt ggtaagcgcc
    10981 tgcgaccttt tctggtttat gccaccggtc atatgttcgg cgttagcaca aacacgctgg
    11041 acgcacccgc tgctgccgtt gagtgtatcc acgcttactc attaattcat gatgatttac
    11101 cggcgatgga tgatgacgat ctgcgtcgcg gtttgccgac ctgccatgtg aagtttggcg
    11161 aagcgaacgc gattctcgct ggcgatgctt tacaaacgct ggcgttctcg attctaagcg
    11221 atgctgatat gccggaagtg tcggatcgcg acagaatttc gatgatttct gaactggcga
    11281 gcgccagcgg tattgccgga atgtgcggtg gtcaggcatt agatttagac gcggaaggca
    11341 aacatgtacc tctggacgca ctcgagcgta ttcatcgtca taaaaccggc gcattgattc
    11401 gcgccgccgt tcgccttggt gcattaagcg ccggagataa agggcgtcgt gctctgccag
    11461 tactcgacaa gtacgcagag agcatcggcc ttgccttcca ggttcaggat gacatcctgg
    11521 atgtggtggg agatactgca acgttgggaa aacgccaggg tgccgaccag caacttggta
    11581 aaagtaccta ccctgcactt ctgggtcttg agcaagcccg gaagaaagcc cgggatctga
    11641 tcgacgatgc ccgccagtcg ctgaaacaac tggctgaaca gtcactcgat acctcggcac
    11701 tggaagcgct agcggactac atcatccagc gtaataaata aacaataagt attaataggc
    11761 ccctgatgag ttttgatatt gccaaatacc cgaccctggc actggtcgac tccacccagg
    11821 agttacgact gttgccaaaa gagagtttac cgaaactctg cgacgaactg cgccgctatt
    11881 tactcgacag cgtgagccgt tccagtgggc acttcgcctc cgggctgggc acggtcgagc
    11941 tgaccgtggc gctgcactac gtctataaca ctccgtttga ccagttaatc tgggatgtgg
    12001 ggcatcaggc ttatccgcat aaaattttga ccggacgccg cgacaaaatc ggcaccatcc
    12061 gtcagaaagg tggcctgcac ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa
    12121 gcgtcggaca ttcatcaacc tccatcagtg ccggaattgg tattgcggtt gctgccgaga
    12181 aagaaggcaa aaatcgccgc accgtctgtg tgattggcga tggcgcgatt accgcaggca
    12241 tggcgtttga agcgatgaat cacgcgggcg atatccgtcc tgatatgctg gtggttctca
    12301 acgacaatga aatgtcgatt tccgaaaatg tcggtgcgct caacaaccat ctggcacagc
    12361 tgctttccgg taagctttac tcttcactgc gcgaaggcgg gaaaaaagtt ttctctggcg
    12421 ttccgcccat taaagagctg ctcaaacgta ccgaagaaca tattaaaggc atggtggtgc
    12481 ctggcacatt gtttgaagag ctgggcttta actacatcgg cccggtggac ggtcacgatg
    12541 tgctggggct tatcaccacg ctgaagaaca tgcgcgacct gaaaggcccg cagtttctgc
    12601 atatcatgac caaaaaaggt cgtggttatg aaccggcaga aaaagaccca atcaccttcc
    12661 acgctgtgcc taaatttgat ccctccagcg gttgtttgcc gaaaagtagc ggtggtttgc
    12721 cgagctattc aaaaatcttt ggcgactggt tgtgcgaaac ggcagcgaaa gataacaagc
    12781 tgatggcgat tactccggcg atgcgtgaag gttccggcat ggtcgagttt tcacgtaaat
    12841 tcccggatcg ctacttcgat gtggcaattg ccgagcaaca cgcggtgacc tttgccgccg
    12901 gtctggcgat tggtgggtac aaacccattg tcgcgattta ctccactttc ctgcaacgcg
    12961 cctatgatca ggtgttgcat gacgtggcga ttcaaaagct tccggtcctg ttcgccatcg
    13021 accgtgcggg cattgttggt gctgacggtc aaacccatca gggcgctttt gatctctctt
    13081 atctgcgctg cataccggaa atggtcatta tgactccgag cgatgaaaac gaatgtcgcc
    13141 tgatgctcta taccggctat cactataacg atggcccgtc agcggtgcgc tacccgcgtg
    13201 gcaacgcggt cggcgtggaa ctgacgccgc tggaaaaact accaattggc aaaggcattg
    13261 tgaagcgtcg tggcgagaaa ctggcgatcc ttaactttgg tacgctgatg ccagaagcgg
    13321 cgaaagtcgc cgaatcgctg aacgccacgc tggtcgatat gcgttttgtg aaaccgcttg
    13381 atgaaacgtt aattctggaa atggccgcca gtcatgaagc actggttacc gtagaagaaa
    13441 acgccattat gggcggtgca ggcagcggag tgaacgaagt gctgatggcc catcgtaaac
    13501 cagtacccgt gctgaacatt ggcctgccgg acttctttat tccgcaagga actcaggaag
    13561 aaatgcgcgc cgaactcggc ctcgatgccg ccggtatgga agccaaaatc aaggcctggc
    13621 tggcataatc cctactccac tcctgctatg cttaagaaat tattcatggc aggagtggca
    13681 gcatgcaata caacccctta ggaaaaaccg accttcgcgt ttcccgactt tgcctcggct
    13741 gtatgacctt tggcgagcca gatcgtggta atcacgcatg gacactgccg gaagagagca
    13801 gccgtcccat aattaaacgc gcgctggaag gcggcataaa tttctttgat accgccaata
    13861 gctattccga tggcagcagc gaagagatcg tcggtcgcgc actgcgggat ttctcccgtc
    13921 gtgaagacgt ggtcgttgcc accaaagtgt tccatcgcgt tggtgattta ccggaaggat
    13981 tatcccgtgc acaaattttg cgctctatcg acgacagcct gcgacgtctc ggcatggatt
    14041 atgtcgatat cctgcaaatt catcgctggg attacaacac gccgatcgaa gagacgctgg
    14101 aagccctcaa cgacgtggta aaagccggga aagcgcgtta tatcggcgcg tcatcaatgc
    14161 acgcttcgca gtttgctcag gcgctggagc tacaaaaaca gcacggctgg gcgcagtttg
    14221 tcagtatgca ggatcactac aatctgattt atcgcgaaga agagcgcgag atgctgccgc
    14281 tgtgttatca ggaaggcgtg gcggtgattc cgtggagccc gctggcacgg ggacgtctga
    14341 cgcgtccgtg gggagaaacc actgcacgac tggtgtctga tgaggtgggg aaaaatctct
    14401 ataaagaaag cgatgagaat gacgcgcaaa ttgcggagcg gttaacgggc gtcagtgaag
    14461 atctgggcgc gacacgagca caagtcgcac tggcatggtt gttgagcaaa cctggcattg
    14521 ctgcaccgat tatcggtaca tcgcgggaag aacagcttga tgagctactg aacgcggtgg
    14581 atatcacttt gaagccagaa cagattgccg aactggaaac gccgtataaa ccgcatccgg
    14641 tcgtaggatt taagtaaatt tcgcttttcg cccattttat ctgctgaatt ccccatcgtc
    14701 tgcgatgatg gggacaacgt acctcccatc accttctgta accataatcc acctctgcaa
    14761 gagaaattct tcgtgattaa atttacagct attcatcatc ttcatcgctt tgag
//
LOCUS       DAZKFU020000113        14739 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZKFU020000113 DAZKFU020000000
VERSION     DAZKFU020000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.114"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(11..87)
                     /locus_tag="ACHOQQ_RS13755"
                     /old_locus_tag="pgaptmp_002751"
     tRNA            complement(11..87)
                     /locus_tag="ACHOQQ_RS13755"
                     /old_locus_tag="pgaptmp_002751"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51..53),aa:Arg,seq:acg)
     gene            complement(286..362)
                     /locus_tag="ACHOQQ_RS13760"
                     /old_locus_tag="pgaptmp_002752"
     tRNA            complement(286..362)
                     /locus_tag="ACHOQQ_RS13760"
                     /old_locus_tag="pgaptmp_002752"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(326..328),aa:Arg,seq:acg)
     gene            complement(425..501)
                     /locus_tag="ACHOQQ_RS13765"
                     /old_locus_tag="pgaptmp_002753"
     tRNA            complement(425..501)
                     /locus_tag="ACHOQQ_RS13765"
                     /old_locus_tag="pgaptmp_002753"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(465..467),aa:Arg,seq:acg)
     gene            complement(701..777)
                     /locus_tag="ACHOQQ_RS13770"
                     /old_locus_tag="pgaptmp_002754"
     tRNA            complement(701..777)
                     /locus_tag="ACHOQQ_RS13770"
                     /old_locus_tag="pgaptmp_002754"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(741..743),aa:Arg,seq:acg)
     gene            complement(781..873)
                     /locus_tag="ACHOQQ_RS13775"
                     /old_locus_tag="pgaptmp_002755"
     tRNA            complement(781..873)
                     /locus_tag="ACHOQQ_RS13775"
                     /old_locus_tag="pgaptmp_002755"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(837..839),aa:Ser,seq:gct)
     gene            complement(1189..1374)
                     /gene="csrA"
                     /locus_tag="ACHOQQ_RS13780"
                     /old_locus_tag="pgaptmp_002756"
     CDS             complement(1189..1374)
                     /gene="csrA"
                     /locus_tag="ACHOQQ_RS13780"
                     /old_locus_tag="pgaptmp_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGU4470980.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1609..4239)
                     /gene="alaS"
                     /locus_tag="ACHOQQ_RS13785"
                     /old_locus_tag="pgaptmp_002757"
     CDS             complement(1609..4239)
                     /gene="alaS"
                     /locus_tag="ACHOQQ_RS13785"
                     /old_locus_tag="pgaptmp_002757"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGU4470981.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4367..4867)
                     /gene="recX"
                     /locus_tag="ACHOQQ_RS13790"
                     /old_locus_tag="pgaptmp_002758"
     CDS             complement(4367..4867)
                     /gene="recX"
                     /locus_tag="ACHOQQ_RS13790"
                     /old_locus_tag="pgaptmp_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGU4470982.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATEEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLSTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(5104..6165)
                     /gene="recA"
                     /locus_tag="ACHOQQ_RS13795"
                     /old_locus_tag="pgaptmp_002759"
     CDS             complement(5104..6165)
                     /gene="recA"
                     /locus_tag="ACHOQQ_RS13795"
                     /old_locus_tag="pgaptmp_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGU4470983.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6245..6742)
                     /gene="pncC"
                     /locus_tag="ACHOQQ_RS13800"
                     /old_locus_tag="pgaptmp_002760"
     CDS             complement(6245..6742)
                     /gene="pncC"
                     /locus_tag="ACHOQQ_RS13800"
                     /old_locus_tag="pgaptmp_002760"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGU4470984.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6887..7972)
                     /gene="mltB"
                     /locus_tag="ACHOQQ_RS13805"
                     /old_locus_tag="pgaptmp_002761"
     CDS             complement(6887..7972)
                     /gene="mltB"
                     /locus_tag="ACHOQQ_RS13805"
                     /old_locus_tag="pgaptmp_002761"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGU4470985.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            8228..8791
                     /gene="srlA"
                     /locus_tag="ACHOQQ_RS13810"
                     /old_locus_tag="pgaptmp_002762"
                     /gene_synonym="gutA"
     CDS             8228..8791
                     /gene="srlA"
                     /locus_tag="ACHOQQ_RS13810"
                     /old_locus_tag="pgaptmp_002762"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HGU4470986.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8788..9747
                     /gene="srlE"
                     /locus_tag="ACHOQQ_RS13815"
                     /old_locus_tag="pgaptmp_002763"
                     /gene_synonym="gutE"
     CDS             8788..9747
                     /gene="srlE"
                     /locus_tag="ACHOQQ_RS13815"
                     /old_locus_tag="pgaptmp_002763"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HGU4470987.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLVQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9758..10129
                     /gene="srlB"
                     /locus_tag="ACHOQQ_RS13820"
                     /old_locus_tag="pgaptmp_002764"
                     /gene_synonym="gutB"
     CDS             9758..10129
                     /gene="srlB"
                     /locus_tag="ACHOQQ_RS13820"
                     /old_locus_tag="pgaptmp_002764"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGU4470988.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            10133..10912
                     /gene="srlD"
                     /locus_tag="ACHOQQ_RS13825"
                     /old_locus_tag="pgaptmp_002765"
     CDS             10133..10912
                     /gene="srlD"
                     /locus_tag="ACHOQQ_RS13825"
                     /old_locus_tag="pgaptmp_002765"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGU4470989.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            11018..11377
                     /gene="gutM"
                     /locus_tag="ACHOQQ_RS13830"
                     /old_locus_tag="pgaptmp_002766"
     CDS             11018..11377
                     /gene="gutM"
                     /locus_tag="ACHOQQ_RS13830"
                     /old_locus_tag="pgaptmp_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGU4470990.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11444..12217
                     /gene="srlR"
                     /locus_tag="ACHOQQ_RS13835"
                     /old_locus_tag="pgaptmp_002767"
                     /gene_synonym="gutR"
     CDS             11444..12217
                     /gene="srlR"
                     /locus_tag="ACHOQQ_RS13835"
                     /old_locus_tag="pgaptmp_002767"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGU4470991.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            12210..13175
                     /gene="gutQ"
                     /locus_tag="ACHOQQ_RS13840"
                     /old_locus_tag="pgaptmp_002768"
     CDS             12210..13175
                     /gene="gutQ"
                     /locus_tag="ACHOQQ_RS13840"
                     /old_locus_tag="pgaptmp_002768"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGU4470992.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLVGAINLQDFYQAGII"
     gene            complement(13172..14686)
                     /gene="norR"
                     /locus_tag="ACHOQQ_RS13845"
                     /old_locus_tag="pgaptmp_002769"
     CDS             complement(13172..14686)
                     /gene="norR"
                     /locus_tag="ACHOQQ_RS13845"
                     /old_locus_tag="pgaptmp_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGU4470993.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAARFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
BASE COUNT         3401 a         4081 c         3704 g         3553 t
ORIGIN      
        1 tcggataata tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag
       61 tactctatcc agctgagcta cggatgcatc gggaaaattt attttactgc tgatattgat
      121 atcgctacta aagccatatc aagtaagaga tggtgcatcc gggaggatta ctcgccttgc
      181 ggctcgccct tcgggccgtt gctggcgcaa cgttatcctc cctggtgcct gcggttaatt
      241 ccgctacctc ttcgatatct tcattgctga agatttcgga gaatatggtg catccgggag
      301 gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat
      361 gcatcgggaa atttatttta ctgctgatat tgataccgct actaaagcca tatcaagtaa
      421 gagatggtgc atccgggagg attcgaacct ccgaccgctc ggttcgtagc cgagtactct
      481 atccagctga gctacggatg catcgggaaa cttactttac tgcagatttt ttgataccgc
      541 tactaaagcc gtatcaagta agagatggtg catccgggag gattactcgc cttgcggctc
      601 gcccttcggg ccgttgctaa agcaacgtta tcctccctgg tgcctgcggt tagtaccgcc
      661 acctcttcca taccttcatc gctgaaggtt acggagaata tggtgcatcc gggaggattc
      721 gaacctccga ccgctcggtt cgtagccgag tactctatcc agctgagcta cggatgcaaa
      781 tggcggtgag gcggggattc gaaccccgga tgcagctttt gaccgcatac tcccttagca
      841 ggggagcgcc ttcagcctct cggccacctc accacacgcc tcttacgagt gcttcgaaga
      901 acttgtttat cgctcatcgt cgctgcgtgg cgcacatatt actttctgag acttataagt
      961 caaacaattt ttcccacact tttatcgttt gcacacttca cgttcaatta gtctgtaaaa
     1021 acgtcaaaaa gagtgtttta tcaacagaaa aatggaggtc tgacagatag cagtaatgta
     1081 aaaaaatgga gacttaagtt gaatgaacgg gagtaaagcg aaaagactat agagtgaagg
     1141 agaaattttg agggtgcgtc tcaccgataa agatgagacg cggaaagatt agtaactgga
     1201 ctgctgggat ttttcagcct ggatacgctg gtagatctct tcacggtgaa cagaaacttc
     1261 cttcggggca tttacgccaa tacgtacctg gttgcccttt acccctaaaa ctgtcacggt
     1321 gacctcatcc ccaatcatga gggtctcacc aactcgacga gtcagaatca gcattctttg
     1381 ctccttgaaa gattaaaaga gtcgggtctc tctgtatccc ggcattatcc atcatataac
     1441 gccaaaaagt aagcaatgac aaacacatta catctaagca gtcatggcat tacattctgt
     1501 taaacctaag tttagccgat atacacaact tcaacctgac tttatcgttg tcgatagcgt
     1561 taatgcgaat gccgtgaagc gagtccacgg cattgcctta cgcttatatt attgcaattt
     1621 cgcgctgacc cagcctttca cactggctaa cgctgcaggt aaggccgcag catccgtacc
     1681 accggcttgc gccatgtcag gacgtccacc acccttgcca cccacctgct gagcgaccat
     1741 accaatcagt tcccctgctt tcacacgatc tgtgacgtcc ttagatacgc ctgcaatcag
     1801 agaaacctta ccttcggcta ccgttgccag cacgataatt gtcgacccca gctgattttt
     1861 taaatcgtca accatggtac gcaacatttt cggctcaaca ccgctaagct cgctaaccaa
     1921 cagcttaaca ccattaacat caattgcctt actggaaaga tttgcgctct cctgtgcggc
     1981 agcttgttct ttaagctgtt gtagttcttt ttctaactga cgcgtacgtt ccagtactga
     2041 gcgcacttta tcagccagat tattgctatc gcctttcagc agatgtgcga cttcgcttaa
     2101 gcgatcgctg tctgcatgaa cggtggcgat agcgccttct ccggttaccg cttcaatacg
     2161 acgaacgcct gcagcagtac ccgattcaga gatgatgcgg aacagaccaa tatcaccagt
     2221 gcggctggcg tgagtaccgc cacacagctc ggtggagaaa tcgcccatgc tcagcacgcg
     2281 tacgcgctca tcatacttct cgccgaacag cgccatcgca cctttcgctt tcgccgcttc
     2341 gagatccatg atgttggttt cgatcggcaa attgcgacga atctgtgcgt tcaccaggtc
     2401 ttcgaccgca cgaatctctt ctggtttcat cgcttcgttg tgtgagaagt cgaagcgcag
     2461 caccttgtcg ttaaccagtg aacctttctg cgatacatga gtacccagaa cctggcgcag
     2521 cgcagcatgc atcaggtgcg ttgcggagtg attcagacga atacgggcgc gacgagcctc
     2581 atcaacatca gcctgcacag cgtcgcccac tttcagagaa cccgcagcaa gtttaccgat
     2641 gtgaccaatc gcctggccgt atttctgcgt atcttccacc acgaaggaga agttagcgcc
     2701 tttcagttcg cctttatcac caacctgacc gccggattcc gcatagaatg gcgtttgatc
     2761 cagcacgacc acagcttcct ggcctgcatt gatggcatca accgctttac catcaacaaa
     2821 cagcgcggtc actttgccgt tcagttccag atggtcatag cctttaaatt cagatgcact
     2881 gtcaacacgg atcattgcgt tgtaatcggc accaaagccg ctggcttcgc gcgcgcgacg
     2941 acgctgctct tccattgcag cttcaaaacc agcttcgtca actttgatgt tgcgctcacg
     3001 acaaacatca gccgtcaggt caaccgggaa gccataggtg tcgtacagac ggaaagcagt
     3061 ttcaccatcc agcgtatcac cagaaagttt tgccagctct tcatccagca acgccagacc
     3121 gcgctccaga gtacgagcaa actgctcttc ttcagtcttc agcacctgct caacctgcgc
     3181 ctgctggcgt ttcaggtctt cacccgcaga gcccataacg tcgatcagcg gaccaaccag
     3241 tttgtagaag aaggtttctt tcgcgccgag catattaccg tgacgcactg cgcgacgaat
     3301 gatacgacgc agtacataac cacggttttc attggacggc attacgccat ccgcgatcag
     3361 gaacgcacaa gaacgaatgt ggtcagcgat tacgcgcagc gatttattgc tcagatcggt
     3421 tgcgccagtg actttcgcta ccgcctggat caacgtgcgg aacaggtcga tgtcatagtt
     3481 agagttaacg tgttgcagca ccgcagcaat acgctccaga cccataccgg tatctacaga
     3541 cggcttcggc agcggttcca tcgtgccatc ggcctggcgg ttgaactgca tgaagacgat
     3601 gttccagatc tcaatgtagc ggtcgccgtc ttcttccgga cttcccggag ggccacccca
     3661 aatgtggtcg ccgtgatcgt agaagatttc ggtgcacggg ccgcacggac cagtgtcacc
     3721 catctgccag aagttgtcag atgcgtatgg cgcacctttg ttatcgccga tgcgaataat
     3781 acgttcgcgc gggatcccga cttctttttc ccagatttcg taggcttcgt cgtcgctttc
     3841 atagacggta acccacagac gctctttcgg cagggcaaac catttttcgc tggtcagcag
     3901 ttcccatgca aactgaatgg catcgtgttt gaaatagtcg ccgaagctga agttgcccag
     3961 catttcgaag aaggtatggt gacgcgcggt gtaaccgacg ttttccaggt cgttgtgttt
     4021 accacccgca cgcacacagc gttgggaagt ggtagcgcgg gaataattac gcttgtcgag
     4081 cccaaggaac acatccttga actggttcat cccggcgttg gtaaacaaca aagttgggtc
     4141 gttatggggt accagggagc tgctggcaac tacctgatgt cccttactat ggaaaaagtc
     4201 gagaaacgcc tgacggatct cagcggtgct cttgctcata attatcctga aatcaagcta
     4261 acgaaatatc gccaccagct ccagcgtgtc ttaaccgccg ggctggtaac tgaaaagtgg
     4321 gaataagata agttttcttg actgggaagt aaaataccgt atgcgttcag tcggcaaaat
     4381 ttcgccaaat atcctggata tcttccatca gatagccacg atagagcaga aaacgctgga
     4441 tcttaacttt ttctgaaaag acagttgaca aaggttcacc atattttcgc gtcgcctgat
     4501 cgcgcgccag tgcgcaccag tcgatgtcac attcacgcat cgctttttct gtcgcttcgc
     4561 gggaaatacc tttctgattc agttcctggc gaatacgcgc aggtccataa cctttgcggc
     4621 tacggctggc gataaagcgc gcaacaaatc ggctgtcatc gagatagcca tgttcatggc
     4681 accaggcaat aacgcgctcg taatcttctt ccgtggcatc aatctcttcc gggccatttt
     4741 tgcccataat cggtgccgcg agtttacgtc gcagttcttg ctcactgtga tcgcgcaccg
     4801 ccagaatgcg taccgcacga tccaacaggc gagcatatgc cgggcgacgg gacgttgatt
     4861 ctgtcatggc atatccttac aacttaaaaa agcaaaaggg ccgcagatgc gacccaggtt
     4921 ttgctgataa acagatttaa tagtcaatga gtgtgcagaa tgttcccgca ggcccaattc
     4981 gttagaggat cgaggtgaga gtaatttttc acgccagtgc tggcctgttc ccctcaccct
     5041 ctccccaaag gggcgagggg accgtccgtg cacacattat ctattatcat caataacgaa
     5101 cgattaaaaa tcttcgttag tttctgctac gccttcgcta tcatctacag agaaatccgg
     5161 cgttgagttc gggttgctca gcagcaactc acgtactttc ttctcgatct ctttcgcggt
     5221 ttccgggtta tctttcagcc aggcagttgc attcgcttta ccctgaccga tcttctcacc
     5281 tttgtagctg taccacgcgc ctgctttctc gatcagcttc tcttttacgc ccaggtcaac
     5341 cagttcgccg tagaagttga taccttcgcc gtagaggatc tggaattcag cctgtttaaa
     5401 cggcgcagcg attttgttct tcaccacttt cacgcgggtt tcgctaccca ccacgttttc
     5461 gccctctttc accgcgccga tacgacggat gtcgagacga acagaggcgt agaatttcag
     5521 cgcgttacca ccggtagtgg tttccgggtt accgaacatc acaccaattt tcatacggat
     5581 ctggttgatg aagatcagca gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat
     5641 cgcctggctc atcatacgtg ccgcaaggcc catgtgagag tcgccgattt cgccttcgat
     5701 ttccgctttc ggcgtcagtg ccgccacgga gtcaacgacg ataacgtcta ctgcgccaga
     5761 acgcgccagg gcgtcacaga tttccagtgc ctgctcgccg gtgtccggct gggagcacag
     5821 caggttgtcg atatcgacgc ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc
     5881 atcgataaac gcacaggttt taccttcacg ctgcgctgcg gcgatcacct gaagcgtcag
     5941 cgtggtttta ccggaagatt ccggtccgta gatttcgacg atacggccca tcggcagacc
     6001 acctgcccca agcgcgatat ccagtgaaag cgaaccggta gagatggttt ccacatccat
     6061 ggaacggtct tcacccaggc gcatgatgga gcctttacca aattgtttct caatctggcc
     6121 cagtgctgcc gccaacgctt tctgtttgtt ttcgtcgata gccattttta ctcctgtcat
     6181 gccgggtaat accggatagt caatatgttc tgttgaagca attatactgt atgctcatac
     6241 agtatcaagt gttttgtaga aattgttgcc acaaggtctg caatgcatac gcagtagcct
     6301 gacgacgcac cgcatcacgg tcgccgctga agcattcccg ccgggtaatg ccttcaccgc
     6361 gggcagtggc aaaagcaaac cagacggtgc cgacaggctt ctcttcactg ccgccatccg
     6421 gcccggcgat accactaata gacacggcgt aatcagcacg agccgctttc agtgcgccta
     6481 tcgccatttc caccacgacg ggttcgctca ccgcgccatg ctgcgccagc gtctcttcac
     6541 gtacgccgat catctgcgct ttggcttcgt tactgtaggt gacaaatccg cgttcaaacc
     6601 aggcggagct accggcaata tcggtaatca ctttcgctac ccaaccgccg gtacaagact
     6661 cagcagttgt tacggttgcg ccacgggctt tcagcgcctg cccaacctgt tcgcttaact
     6721 gcatcagttc actgtcagtc atcacaacct ctgtcagtca aaaatttatg ccggacaaga
     6781 tagcactttc gtcaggaaga tggtgttggc gttgcaattt tacgaggagg attcagaaaa
     6841 aagctgatta gccagaggga agctcacgct cccctcttgc aaatagttac tgcacgcgcg
     6901 ccagcgccac ggcttgtcct aactgccaga ccgccattgc gtaatgggtg ctgtggttat
     6961 aacgggtaat ggtgtagaag ttcggcagac cgtaccagta ctggtaaccg gtaccgacat
     7021 ccagacgcag caggctggct tgttgatggt tgcccagcgg ctgctgtggc gttaaacctg
     7081 ccgcggcaag ctgcgaaatg ctgtacttgg ttttgaagcc atttggcaag cctggagcct
     7141 gaccgtttgc cattaccgcg acctgatcgc ctttcaccca accgtgcgct ttgaaataat
     7201 tcgccacgct accgatcgca tcaaccggat cccacaggtt gatatgcccg tcgccgctga
     7261 aatctaccgc atattgtttg taagacgacg gcataaactg tccgtagccc atcgccccgg
     7321 caaaggaacc tttcagattg agcggatcgt cctgctcgtc gcgcgccatc agcaggaagg
     7381 tttccagctc gccagagaaa tactccgcgc ggcgtgggta gttaaatgac agcgttgcca
     7441 gcgcatcgag gatgcgagtt ttccccatca cgcgccccca gcgggtttca acgccgataa
     7501 tcccgacgat aatttccggc ggtacgccat acacctgcca cgcgcgattc aacgcatctt
     7561 catactgatt ccagaaaacc acaccgttct gcacgttgtc cggcgtaata aattttttgc
     7621 gataacgcag ccatgcgccg ttcggacctg atggcggttt caccgatgtg gttggtgcct
     7681 ggttatccat cagccgcagt accgaatcca gacgtttcgc ctgggagaga atttcctgca
     7741 actgctgacg atcgaaaccg tgtttgttca ccattttgtc gatgaactgc tgggcattcg
     7801 ggttattggc gaagtcaccg cccatctgca tcacattgtg ctgcggctca agcaggaagc
     7861 cgccagacgg cgttccggtg gtcgtttcag tctcagtagg ttttggcttg ctgctacagg
     7921 cggcaagcaa cacaaaaagg ggaagcaatg ttacataacg acgcttgaac atgaggggtc
     7981 catttaacag attcaaccag gggcaagtat ggtaaagcat cacgccccgc acaaggaagc
     8041 ggtagtcact gcccgatacg gactttacat aactcaactc atccccctcg ctatcctttt
     8101 attcaaactt tcaaattaaa atatttatct ttcattttgc gatcaaaata acacttttaa
     8161 atctttcaat ctgattagat taggttgccg tttggtaata aaacaataaa tcctgaagga
     8221 gagaacaatg atagaaacca ttactcatgg tgcagagtgg tttatcgggc tgttccaaaa
     8281 gggcggagag gtgtttaccg ggatggtgac cggcattctt ccgctgttga ttagcctgct
     8341 ggttatcatg aacgcactga ttaattttat cggtcagcat cgaattgaac gttttgctca
     8401 acgttgcgcc ggtaaccctg tttcccgtta cctgctgcta ccgtgcattg gcacgtttgt
     8461 cttttgcaat ccgatgaccc taagtctggg tcgctttatg ccggaaaagt acaaacccag
     8521 ctactacgcg gccgcctctt atagctgcca ctcaatgaat ggcctcttcc cccatatcaa
     8581 ccctggcgaa ctgtttgttt atcttggcat tgccagcggt ctgacaacgc tgaacctgcc
     8641 acttggccca ctggcggtga gctatctgct ggttggtctg gtcaccaact tcttccgcgg
     8701 ctgggtgacc gatctgacca ccgccatttt tgagaaaaag atgggtattc aacttgaaca
     8761 aaaagttcac ctggcaggag caacatcatg acgcgtattc ggatcgaaaa aggaacgggt
     8821 ggctggggcg gcccgcttga gctggaagcc acgccgggca aaaaaatcgt ctatatcacc
     8881 gccggtaccc ggcctgcgat tgttgacaaa ctggtacagc ttactggctg gcaggctatt
     8941 gacggattta aagaaggtga acccgcggag gcggaaattg gtgtcgcggt aatcgactgt
     9001 ggcggcacat tacgctgcgg catttatccg aaacggcgta ttcccaccat taatatccac
     9061 tcgacgggca agtccggtcc actggcgcag tacattgtgg aagatattta tgtctctggc
     9121 gtaaaagaag aaaacatcac tgtagtgggt gatgcgacac cacaaccctc ttccgtgggc
     9181 cgtgactatg acaccagcaa gaaaatcacc gaacaaagcg atggtttact ggcgaaggtg
     9241 ggaatgggta tgggttctgc cgttgccgtg ttgtttcaat ctggtcgtga caccatcgac
     9301 actgtattaa aaaccattct gccgtttatg gcattcgtct cggcgctcat tggcatcatt
     9361 atggcttctg gccttggtga ctggattgcc cacggtcttg ctccgctggc gagccatcca
     9421 ctgggtctgg tcatgctggc gctcatctgc tccttcccgc tgctttcacc tttcctcggc
     9481 ccaggcgcag ttatcgcaca ggttatcggc gtattgattg gcgtgcagat tggtctcggc
     9541 aatattccgc cgcatctggc tttacctgcg ctgtttgcca tcaacgcgca ggcggcctgc
     9601 gacttcatcc cggtcggttt gtcgctggcg gaagcccgtc aggacacggt tcgcgtcggt
     9661 gtcccttctg ttctggtgag ccgcttttta accggcgcac cgactgtact gatcgcctgg
     9721 tttgtctccg gttttatcta tcaatagagg ctgaaacatg accgttattt atcagaccac
     9781 catcacccgt atcggcgcga gtgccactga cgccctcagc gaccagatgc tcatcacctt
     9841 tcgtgaaggc gcgcctgcgg atctcgaaga gtattgcttc attcattgcc acggcgagtt
     9901 gaaaggtgca ctccatcccg gtttgcaatt ttcgctcggg cagcatcgct atccggtgac
     9961 cgccgttggc agcgtggcgg aagacaacct tcgcgaactg ggtcatgtca ccctgcgctt
    10021 cgatggttta aacgaagcgg aatttccggg cactgtccat gtggcaggcc ctgtccccga
    10081 cgatatcgcg ccgggatcgg ttttgaagtt tgaatctgtt aaggagtaaa aaatgaatca
    10141 ggttgccgtt gtcatcggtg gtgggcaaac cttaggcgcg ttcctgtgcc acggtctggc
    10201 tgccgagggg tatcgcgtcg cggttgtcga tattcagagc gacaaagccg caaatgtggc
    10261 acaagaaatt aacgccgaat atggtgaagg tatggcgtac ggttttggtg ctgacgccac
    10321 cagtgagcaa agcgttctgg cgctctctcg tggggtagat gaaatctttg gtcgcgtgga
    10381 tttgctggtc tacagcgccg gaattgcaaa agcagccttt atcagcgact tccagctcgg
    10441 tgattttgac cgttcgctac aggtgaatct ggtgggttat ttcctgtgtg cgcgtgaatt
    10501 ttcgcgtttg atgatccgcg atgggattca gggacgcatt attcagatca actcgaaatc
    10561 cggcaaagtg ggcagcaaac acaactctgg ctacagcgca gcgaaatttg gtggcgtagg
    10621 gctgactcaa tcactggcgc tggatctggc ggagtacggc attacggtgc attcactgat
    10681 gctcggtaac ctgctgaaat cgccgatgtt ccagtcgctg ttgccacaat acgcgaccaa
    10741 gctgggtatc aaaccggatc aagtcgagca gtattacatc gataaagtac cgctcaaacg
    10801 cggctgcgat tatcaggatg tgctgaatat gctgctgttc tacgccagtc ctaaggcatc
    10861 gtactgcact ggtcagtcga tcaatgtcac cggcggtcag gtgatgttct gatcaacagc
    10921 ggagatccgt taaggatctc cgtgggacta atagaatgcc tgatgcgcta cgctcatcag
    10981 gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac tcatcactgt
    11041 cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt ctcgttttaa
    11101 ccgtgccttc gacacactat gccagcaagg gcgggttggc gtgggccgtt ccagcgggcg
    11161 ctttaaaccg cgggtcgtgg tcgccatcgc cctggacgat cagcagcgca tcgccgacac
    11221 cttgtttatg aaaggactga ccgtcttcgc ccgaccgcaa aaaattcccg caattaccgg
    11281 catgcatgcg ggtgatttac agcccgatgt gatctttccc catgatccac tatcacagaa
    11341 tgctctatca ttggcgctta aactgaaacg tggataattt cgttgtgaat gttacttgct
    11401 tgcgaagtta tcattttgaa acctaaatca ggtaatcacg cccatgaaac ctcgtcagcg
    11461 tcaggccgcc attctggagt atctgcaaaa gcagggtaaa tgctcggttg aagaattggc
    11521 gcaatacttt gacaccacag gcacaaccat tcgcaaagat ctggtcattc tggaacatgc
    11581 cggaaccgtc attcgtactt atggcggagt ggtgttgaat aaagaggaat ccgatccgcc
    11641 tatcgatcat aaaacactca tcaacaccca caagaaagag ctgatagcag aagctgccgt
    11701 tggttttatt catgatggcg attcgatcat tcttgatgct ggcagcaccg ttttgcagat
    11761 ggtgcctctg ctctcgcgct ttaataacat cacggtgatg accaacagcc tgcatatcgt
    11821 caatgcgcta tccgaactgg ataacgaaca aactatcctg atgccaggcg gaacgtttcg
    11881 taaaaaatcg gcctcatttc acgggcagct ggcagagaat gccttcgagc atttcacctt
    11941 cgacaaatta ttcatgggta ctgacggcat cgatctcaat gcgggcgtaa ccaccttcaa
    12001 cgaggtctat accgtcagta aggcaatgtg caatgccgcg cgcgaagtga ttttgatggc
    12061 ggactcatca aagtttggcc gtaaaagccc caacgtagtt tgcagtcttg aaagcgtcga
    12121 taagctgatt accgacgcag atatcgatcc ggcgtttcgt caggcgctgg aagagaaagg
    12181 gatcgatgtg atcataaccg gagagagcaa tgagtgaagc actactgaac gcgggacgtc
    12241 agacgttaat gctggaattg caggaagcaa gccgtttacc ggaacgtctg ggcgatgatt
    12301 ttgttcgcgc cgccaatatc atcctgcact gcgaaggcaa agtggtggtt tcgggaattg
    12361 gcaaatcggg ccacattggt aagaaaatcg ccgcaacgct tgccagtacc ggcacaccgg
    12421 ctttttttgt ccatccggca gaagcgctgc acggcgatct ggggatgatc gaaagccgcg
    12481 atgtgatgct gtttatctct tactccggtg gggcgaagga actggatctg attattccgc
    12541 gtctggaaga caaatctatc gcgctgctgg cgatgaccgg caaaccgacg tcaccgctgg
    12601 gcctggcggc gaaagcggtg ctggatatct ccgtagaacg cgaagcctgc ccgatgcacc
    12661 ttgcgccgac ctccagcacc gtcaataccc tgatgatggg tgacgcgctg gcgatggcag
    12721 tcatgcaggc gcgcggattt aatgaagaag attttgcccg ctcccaccct gctggagcac
    12781 tgggcgctcg cttgctgaat aaagtgcatc atctgatgcg ccgtgacgat gccatcccac
    12841 aggtggcgtt aaccgccagc gtgatggatg cgatgctgga actcagccgc accggtctgg
    12901 ggctggtggc ggtatgtgac gctcaacaac aggtacaagg cgtctttacc gacggcgatt
    12961 tacgtcgctg gctggttggc ggcggcgcac tcaccacgcc agtcaatgaa gcgatgacga
    13021 caggcggcac cacattacag gcacaaagtc gcgccatcga cgccaaagag atcctgatga
    13081 agcgcaaaat cactgccgca ccggtggtgg atgaaaacgg caaactcgtc ggcgcaatta
    13141 acctgcagga tttctatcag gccgggatta tttaatcctt caatcccaga cgtttcgcca
    13201 gccgatgcag gttggcgacg tcggtttcca gcattcgcgc gcaggctgcc cagttgtgat
    13261 gattttgtgc cagtgcctga cgaatagttt cacgctggaa cgcttctgtc gcttcacgca
    13321 ggttttgctt aacaacgggc accgccgccg cttctggcgg cggtaacgtc acctcaggaa
    13381 aagcaaaatg ttgcgcctca agaatcactt catcgccgtt gcgggtggct ctcgccagca
    13441 ctaccgcccg atgaatagca tgttccagtt cgcgcacgtt ccccggaaaa cgatagtgtt
    13501 gcagtaaatt tcgcgctccg gcacttaata ccacgcggga gagcccctgc cgcaaacgac
    13561 actgctcgca gaaatacccc gccagcagaa tgacatcatc gccccgctca cgcagcggcg
    13621 gcaccgaaag tggaaacacg ctcaggcggt ggaacaagtc agcgcgaaat cgccctgcca
    13681 gcacctcttc gcgtaaatcg cggttagtcg ccgccagcac gcgcacatcg acccgcaaac
    13741 tacggtcatc gccaacgcgc tgaatatcgc catactgcaa caccctcagc agcttggcct
    13801 gcaatgccaa cgacaactcg ccgatctcat ccagaaacag cgtgccgtta tccgccattt
    13861 caaacttccc actgcggtta ctgattgcgc cagtaaacgc tcctttcaca tgcccgaaca
    13921 actcactttc cgccacactt tccggcagtg cggcacagtt gagatagacc agcggattca
    13981 ccgcccgtgg cgaggcctca tgaatcgctt tcgccaccag ctccttaccg gttcccgtct
    14041 caccgctgat caggacgttg agatcggacg ccgccacaat ctcaatctct tttttcagtt
    14101 gcgtcatgcc tggcgacaga ccgatcatct gcgtctgttt caccgcttca aatcgagcgg
    14161 catcgcccgg cagcatattc tggctttcca gttgctcaat cagcaacgca ttgcttaacg
    14221 ctcccgccgc cagcgcggca atcaggcgta actcttcgtc gctgaaaaca tcgaactgat
    14281 cgggctgcat cccgtcgagc gtcaatgcgc cgatcaggtt ttgcccggca aacagcggca
    14341 gaccaacgca ggcgtgaacc ttcagactct cctgccctgg aatcaaaccg tcatagggat
    14401 cgggcaattc gctgtctgcg ggaaagcgca ccacatcacc cgcgcgggca atcgcttcca
    14461 gccgtggatg accttccagc gcaaagcgtc taccgagtac atccttcgcc agcccgtcga
    14521 tggcaagcgg aataaactgc cgcgagtcgt aacgtagcaa cgcagacgca tcgcactcca
    14581 gcacctgacg cagcgtggtg atcaggcgct gaaaacgatc ctgatgacca atcccacgct
    14641 gcaattcgat ggcgatattc gccagcacat caacggaaaa actcatcttt gcctcactgt
    14701 caatttgact atagatattg tcatatcgac catttgatt
//
LOCUS       DAZKFU020000114        14562 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZKFU020000114 DAZKFU020000000
VERSION     DAZKFU020000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.115"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            57..1049
                     /gene="asnA"
                     /locus_tag="ACHOQQ_RS13850"
                     /old_locus_tag="pgaptmp_002770"
     CDS             57..1049
                     /gene="asnA"
                     /locus_tag="ACHOQQ_RS13850"
                     /old_locus_tag="pgaptmp_002770"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGU4470994.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(1054..2505)
                     /gene="viaA"
                     /locus_tag="ACHOQQ_RS13855"
                     /old_locus_tag="pgaptmp_002771"
     CDS             complement(1054..2505)
                     /gene="viaA"
                     /locus_tag="ACHOQQ_RS13855"
                     /old_locus_tag="pgaptmp_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGU4470995.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRNRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSKEWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(2499..3995)
                     /gene="ravA"
                     /locus_tag="ACHOQQ_RS13860"
                     /old_locus_tag="pgaptmp_002772"
     CDS             complement(2499..3995)
                     /gene="ravA"
                     /locus_tag="ACHOQQ_RS13860"
                     /old_locus_tag="pgaptmp_002772"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGU4470996.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQACAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            4218..6086
                     /gene="kup"
                     /locus_tag="ACHOQQ_RS13865"
                     /old_locus_tag="pgaptmp_002773"
     CDS             4218..6086
                     /gene="kup"
                     /locus_tag="ACHOQQ_RS13865"
                     /old_locus_tag="pgaptmp_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGU4470997.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            6253..6672
                     /gene="rbsD"
                     /locus_tag="ACHOQQ_RS13870"
                     /old_locus_tag="pgaptmp_002774"
     CDS             6253..6672
                     /gene="rbsD"
                     /locus_tag="ACHOQQ_RS13870"
                     /old_locus_tag="pgaptmp_002774"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGU4470998.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            6680..8185
                     /gene="rbsA"
                     /locus_tag="ACHOQQ_RS13875"
                     /old_locus_tag="pgaptmp_002775"
     CDS             6680..8185
                     /gene="rbsA"
                     /locus_tag="ACHOQQ_RS13875"
                     /old_locus_tag="pgaptmp_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGU4470999.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            8190..9155
                     /gene="rbsC"
                     /locus_tag="ACHOQQ_RS13880"
                     /old_locus_tag="pgaptmp_002776"
     CDS             8190..9155
                     /gene="rbsC"
                     /locus_tag="ACHOQQ_RS13880"
                     /old_locus_tag="pgaptmp_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU4471000.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            9180..10070
                     /gene="rbsB"
                     /locus_tag="ACHOQQ_RS13885"
                     /old_locus_tag="pgaptmp_002777"
     CDS             9180..10070
                     /gene="rbsB"
                     /locus_tag="ACHOQQ_RS13885"
                     /old_locus_tag="pgaptmp_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGU4471001.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            10196..11125
                     /gene="rbsK"
                     /locus_tag="ACHOQQ_RS13890"
                     /old_locus_tag="pgaptmp_002778"
     CDS             10196..11125
                     /gene="rbsK"
                     /locus_tag="ACHOQQ_RS13890"
                     /old_locus_tag="pgaptmp_002778"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU4471002.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANISFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLKSVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            11129..12121
                     /gene="rbsR"
                     /locus_tag="ACHOQQ_RS13895"
                     /old_locus_tag="pgaptmp_002779"
     CDS             11129..12121
                     /gene="rbsR"
                     /locus_tag="ACHOQQ_RS13895"
                     /old_locus_tag="pgaptmp_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGU4471003.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(12087..13514)
                     /gene="mdtD"
                     /locus_tag="ACHOQQ_RS13900"
                     /old_locus_tag="pgaptmp_002780"
     CDS             complement(12087..13514)
                     /gene="mdtD"
                     /locus_tag="ACHOQQ_RS13900"
                     /old_locus_tag="pgaptmp_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGU4471004.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(13537..14229)
                     /gene="yieP"
                     /locus_tag="ACHOQQ_RS13905"
                     /old_locus_tag="pgaptmp_002781"
     CDS             complement(13537..14229)
                     /gene="yieP"
                     /locus_tag="ACHOQQ_RS13905"
                     /old_locus_tag="pgaptmp_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGU4471005.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         3524 a         3587 c         3848 g         3603 t
ORIGIN      
        1 atgtgtatcg gtttttgttg cttaatcata agcaacagga cgcaggagta taaaaaatga
       61 aaaccgctta cattgccaaa caacgccaaa ttagcttcgt gaaatctcac ttttctcgcc
      121 aactggaaga acgtctgggg ctgatcgaag tccaggcgcc gattcttagc cgtgtggggg
      181 atggcacgca ggataacttg tcgggctgtg aaaaagcggt gcaggtaaaa gtgaaagctc
      241 tgcctgatgc ccagttcgaa gtggttcatt cactggcgaa gtggaaacgt cagaccttag
      301 ggcaacacga cttcagcgcg ggcgaagggc tgtacacgca catgaaagcc cttcgccccg
      361 atgaagaccg tctttctccg ttgcactcgg tctatgttga ccagtgggac tgggaacgcg
      421 taatgggcga cggtgagcgt caattctcga ctctgaaaag cacggtagag gcgatctggg
      481 cgggaattaa agcaaccgaa gctgcggtta gcgaagagtt tggcctggca ccgttcctgc
      541 cggatcagat ccacttcgta cacagccagg agttactgtc tcgttatccg gatcttgatg
      601 ccaaagggcg tgagcgggcg atagcgaaag atcttggtgc ggtattcctt gtcgggattg
      661 gcggcaagct gagcgatggt catcgtcacg acgtgcgcgc accggattat gatgactgga
      721 gcaccccgtc agagctgggc catgcgggcc tgaacggcga tattctggtg tggaacccgg
      781 tactggaaga tgcgtttgag ctttcttcca tggggatccg cgtggatgcc gacacgttga
      841 agcatcagtt ggcgctgacc ggtgatgaag atcgtctgca gctggaatgg catcaggcgt
      901 tgctgcgcgg tgaaatgccg cagaccatcg gcggcggtat cggccagtct cgtttgacca
      961 tgctgctgct gcaactgccg catatcggcc aggttcagtg tggagtatgg ccagctgctg
     1021 ttcgcgagag cgtcccttct ctgctgtaat aatttatcgc cgccagcgtc tgagcaggcg
     1081 gcttcgcatc ccggtatcaa agcgccagat atgatcgaaa atgcgcatga tgccgggttt
     1141 gccgtgtgcc gacatcgcca cggcatgaaa gcgatgctga tgtacccgct gcagctcttt
     1201 cactttactc gtcacgtcgt caggcaaccg ctgggcgata aaatcagaaa tcaccaccgc
     1261 atcggcatca aaccattcct tgctttgcaa gcgttccata atggcgcgaa aacaactggc
     1321 aagatcggtg ccgccacgaa actgctggct taaaaaacgg attgcttgtt cgatgccttg
     1381 tgggcctgaa agctcataac ggacgatctc ggtggaaaat agcataatat aacagcgccg
     1441 gttttcagcg agagcaatgc gcatcaaagc caggcagaac gctttcgcac actgttcatt
     1501 aaagccgccc attgagccgg aagtatccac gcagacaata aacggtccgc gcggctgttc
     1561 gtcgtaatct ttatgcacca ccgggcgttc gatcactttt tcacgccacg actcaccgtg
     1621 caggcgatag gtgagcaact gtttttccac cagccgacgg taaaactcat actccagttc
     1681 cgttatccct agtgtcgcca gttctggcgg cagaagacgt aaaatatcat cgctttgttg
     1741 cagaccatca acctgctcag gaacagttgc cggttcgcgc accatggtgc ggaaggtttc
     1801 catctgcgca tcgttgcgcg gtattgattt ggcttcccgg gaacgcccca gctgttctgc
     1861 caggcgtttc agttccggct gttcgttaag aaattcaccg tatttcacaa tcaactgata
     1921 gtcgccacgt ttaagctgac cggcgctcat atcccacaga cgaccagctg cggtattgtt
     1981 atctgcgaga atcggttcaa gttgtccgct cagcgtcatg cgttcctgaa cttcactcaa
     2041 cagttgttcg cgttcttctt ctaataactg ttgatttaac gtcgttgctt gcacgatcag
     2101 acttaaacgc caacgctgga ggaaaagcgt gtgtaacgct gaagtgatcg tgctgttagc
     2161 atcaaccaac tgtcgggctt gttctgccca tggggaattc agacgatgca gtaagtccag
     2221 gatctgtggt agctgcacaa taaactgcgg cgtggagagg agctggcttt gctgatagca
     2281 catcacctct tcggtgagtt ccggcgggac tcgggcatct ttcagccgat tacgcagcgc
     2341 ctcacgccag cggggaacat catcagtgat tgccgccttc agtcgtggga atttttcaaa
     2401 gaagaccgct agctgcggtg aggccagcag cgcgatgatc atctcttcga tcaatccctc
     2461 ttcgctgacg gccagcatca cattaagcgt atccagcgtt agcattgttg tgcctggcga
     2521 atctgtgcgc cgacatcctg taggctggct tcaatgcgac ctaaccagtc gccagggata
     2581 aacagacatt tttgctgttc gctgaataaa gcgtgttgct tacgccagtc gctttcaagc
     2641 tcttccagtt gttgttttat ttcacctggc agaccttcag ccgatgaacc gggaagagcc
     2701 agcgtactgc cttgtaaact tacatcgcgt acaacaagat gttgggtgct atcaacttcc
     2761 agattcagtt tctgggcaaa gccgataccg ttcagtttgc cgcgaatttc accaccttta
     2821 ctcagccact gttccagcgc gctacgctca aaggagatat gaaccacttc catatcatgc
     2881 agttttaacg gtttttgcag cagcagagtc agagtggaag cagtaacgtt aacagggagt
     2941 tgatactgct gacgacggct gaaaatgccg cccagacgaa ttaccgttaa ggctgtttta
     3001 tcactttgtt gctgctggag ttgcaggtga cgttgcacaa tcgcgccaag gcgagtcaac
     3061 atcccttgct gttgccaggc gtgaccggtc atcaatacat caatttgttg ttgtatcaaa
     3121 ttcaggcttt gcgcgtcata ccacaggcaa tctttcagca aaatgagatc taccggggca
     3181 acagcactgc gaccgctaaa aaaggcgcag gcctgcaata agcggatcgc ttttttccag
     3241 cgacgatccg agacataagg cgcatccggt aatttatcca gttgctggcg cagcataaaa
     3301 atcagctcaa atacatgatc gggcagcgta atttcaccaa tctctttctg ccagcgttca
     3361 tattcttcat ctgtgacctg caaggcatca ggaaccggat tgtcgttttc atcctgttga
     3421 ctggtcagca tggagcggaa attcgcttta tcctgcactt tatctaacca cagacgaatc
     3481 agcatgcggt catataacgc ttccagactg ctgtctgctt ccggcagctc gttggaggcc
     3541 gccaccagta gacgcatcgg gattttttct acgtgtgcac cgttgcggaa ctggcgctcg
     3601 ttaatggcgg tgagcaaggt attaagaatt gccgggcccg ctttccagat ctcatccaga
     3661 aagacaattt ctgcttccgg caggtaaccg ctggttaaac gttcatagcg cccttcatct
     3721 tttagcgcct gaatagaaag gggaccaaaa acttcttccg gcgtggagaa gcgagtcatc
     3781 agatattcaa acgcgcgggc attctgaaag gcgaatttta agcgccgggc gatcaaactt
     3841 ttggcaatac ctggcgggcc aaggaggaac acactttcac cacttaatgc cgctaataaa
     3901 cacaagcgga tggcgtggct acgttcataa agcccctttt ccaacgaact gctcaggcgg
     3961 gaaattcttt ccgctaataa atgagggtga gccataatga agtggcgtcc tttcgtcaaa
     4021 agttctgcgt aaattgcgag tatagacgtt tcttgctggt ggctaaaata gtctcaaagg
     4081 ggggggattt ttctttgagc caggttaatg tggccgcatt taggagtacg attttgccgt
     4141 taatcgtgca tactgtgcgc ttttttgtgg gccaagggac taagcacaca tttcatattt
     4201 caacgaaaga ctagtctatg agcactgata ataagcaatc attgcccgcg attaccctcg
     4261 cggcgattgg agttgtctac ggcgatattg gtaccagccc gttatataca cttcgtgaat
     4321 gtttgtccgg ccagtttggt tttggcgttg aacgcgatgc cgtgtttggc tttttatcgc
     4381 tgatcttctg gctgctaatc tttgtggttt ccattaaata tctcaccttc gtgatgcggg
     4441 cagataacgc cggtgagggg gggatcctga cgttgatgtc gcttgccggg cgtaatacgt
     4501 cggcgcgaac cacatcaatg ctggtgatta tggggctaat cggcggcagt tttttctatg
     4561 gtgaagtcgt cataacaccc gctatttcgg tgatgtcagc cattgaaggt ctggaaatcg
     4621 tcgccccgca gctggatacc tggatagttc ccctctcaat tatcgttctc actttattat
     4681 ttatgattca aaagcatggc acggctatgg tcggtaagct gtttgcgccg atcatgctga
     4741 cctggttttt gattctggca ggactggggt tacgtagcat tattgctaac ccggaagtgc
     4801 tgcatgcgct gaatccgatg tgggcggtgc atttcttcct tgaatacaaa acggtttctt
     4861 ttattgcatt aggggcagtg gtgctgtcga ttacgggggt cgaggcgctg tatgctgata
     4921 tgggacactt tggtaagttc cctattcgtc tggcgtggtt cactgtcgta ttaccttcct
     4981 taactcttaa ttacttcggc cagggagcgc tgttgttaaa gaacccggaa gcgattaaga
     5041 acccgttctt ccttttggca ccggactggg cgctgatccc gctgctgatc atcgccgcac
     5101 tggcgacggt aatcgcctcg caggcggtta tctctggcgt cttttcattg acgcgtcagg
     5161 cggtacgtct gggatatttg tcgccgatgc gcattattca cacctccgaa atggagtcag
     5221 ggcaaatcta tattccgttt gtgaactgga tgctctatgt cgcggtcgtg attgtgattg
     5281 tcagctttga gcactccagc aacctggcgg cggcgtacgg gattgcggtg accggaacca
     5341 tggtgctgac gtctattctc tcgactaccg tggcacgtca gaactggcac tggaataagt
     5401 attttgttgc gctgatcctg attgctttcc tttgtgtcga tattccattg ttcaccgcta
     5461 acctcgataa actgctctcc ggcggctggt tgccattgag cctcggtact gtgatgttta
     5521 tcgtgatgac cacctggaag agcgagcgtt tccgcttgct gcggcggatg catgaacatg
     5581 gtaactctct ggaagcgatg attgcttcgc tggagaaatc accgcctgtt cgcgtgcccg
     5641 ggaccgcggt gtatatgtcg cgtgcaatca acgtcattcc ctttgcgctg atgcataacc
     5701 tcaaacataa caaggtattg catgagcggg tgattctgtt aactctgcgc accgaagacg
     5761 cgccatatgt ccataacgtc cgtcgggtac agattgaaca actgtcgccc actttctggc
     5821 gcgtggtggc aagttatggt tggcgagaaa cgccaaacgt ggaagaagtt ttccaccgct
     5881 gcggtctgga aggattaagt tgccggatga tggaaacctc cttctttatg tcgcatgagt
     5941 cgttgatcct cggcaaacgc ccgtggtatt tgcgtctgcg cggcaagctg tacttgctgc
     6001 tgcaacgtaa tgcgctgcgt gcaccagatc aatttgaaat cccgccaaac agggttatcg
     6061 aactgggtac tcaggtcgaa atctaacgcc agacgcctcc tttcttcata agggggcgtt
     6121 tttgttttca tggttaatca ccatgtaaaa cgtttcgagg ttgatcacat ttccgtaacg
     6181 tcacgatggt tttcccaact cagtcaggat taaactgcgg gtcagcgaaa cgtttcgctg
     6241 atggagaaaa aaatgaaaaa aggcaccgtt cttaattctg atatttcatc ggtgatctcc
     6301 cgtctgggac ataccgatac gctggtggtg tgtgatgctg ggttacccat ccccaaaagt
     6361 acaacgcgta tcgatatggc attaacccag ggtgtacctt cttttatgca ggtgctgggc
     6421 gtcgtcacaa atgaaatgca ggtcgaggcg gccattatcg cggaagagat caaacaacat
     6481 aatccgcaac tccacgaaac gttgctcact caccttgagc agctgcaaaa acaccaggga
     6541 aataccattg aaattcgtta caccacgcat gaacaattca aacaacaaac cgcagaaagt
     6601 caggcggtaa ttcgcagcgg agaatgttct ccgtatgcga atatcattct ctgtgctggc
     6661 gtgacgttct gaggccgtca tggaagcatt acttcagctt aaaggcatcg ataaagcctt
     6721 cccgggcgta aaagccctct cgggcgctgc gttaaatgtc tatccgggcc gcgtgatggc
     6781 gctggtgggc gaaaacggcg cgggtaaatc caccatgatg aaagtgctta ctggtatcta
     6841 tactcgcgat gccggcacgc ttttatggct ggggaaagaa acgacattta ccgggccgaa
     6901 atcttcccag gaagccggga ttgggattat ccatcaggaa ctgaacctga tcccgcagtt
     6961 gaccattgcc gaaaacattt tcctcggtcg tgagtttgtt aatcgctttg gcaaaattga
     7021 ctggaaaacc atgtatgccg aagcggataa attgctggct aaacttaacc tgcgctttaa
     7081 aagcgacaag ctggtgggcg atctttccat cggtgaccag caaatggttg aaatcgccaa
     7141 agtgctgagc tttgagtcga aagtcatcat tatggatgaa ccgaccgatg cgctgaccga
     7201 taccgaaacc gaatccctgt tccgcgtcat ccgcgagctg aaatcgcaag gccgcggtat
     7261 tgtctatatc tcccaccgca tgaaagaaat cttcgagatt tgcgatgacg ttaccgtttt
     7321 tcgtgacgga caatttattg ctgagcgcga agtggcatca ctgaccgaag attcgctgat
     7381 tgagatgatg gtgggtcgca agctggaaga tcaatatccg cacctggaca aagcgccggg
     7441 agatatccgc ctgaaagtcg ataatctctg cggacctggc gttaacgatg tctcttttac
     7501 tttacgcaaa ggcgaaattc ttggcgtctc tggtttgatg ggcgcaggtc gtaccgaact
     7561 gatgaaagtg ctctacggcg cactaccgcg caccagcggt tacgtcaccc tggatgggca
     7621 tgaagtcgtt acccgttcac cgcaggatgg cctggcaaac ggcattgtgt atatctccga
     7681 agaccgtaaa cgtgacggtt tagtgttggg catgtcagta aaagagaaca tgtcgctgac
     7741 agcgctgcgc tacttcagcc gcgctggtgg cagtttgaag catgccgatg aacagcaggc
     7801 tgtgagtgat ttcattcgtc tgtttaatgt gaaaactccg tcgatggaac aggcaattgg
     7861 tctgctttcc ggtggcaatc agcaaaaagt ggcgattgcc cgcggtctga tgacacgccc
     7921 caaggtgttg atcctcgatg agcctacccg tggcgtagat gtcggcgcga aaaaagagat
     7981 ctatcaactg attaaccagt tcaaagccga tggcttgagc atcattctgg tgtcatcgga
     8041 gatgccagaa gtattaggca tgagcgatcg catcatcgtc atgcatgaag ggcatctcag
     8101 cggggaattt actcgtgagc aggccaccca ggaagtgtta atggctgccg ctgtgggcaa
     8161 gcttaatcgc gtgaatcagg agtaaaaaaa tgacaaccca gactgtctct ggtcgccgtt
     8221 atttcacgaa agcgtggctg atggagcaga aatcgcttat cgctctgctg gtgctgatcg
     8281 cgattgtctc gacgttaagc ccgaactttt tcaccatcaa taacttattc aatattctcc
     8341 agcaaacttc ggtgaacgcc attatggcgg ttgggatgac gctggtgatc ctgacgtcgg
     8401 gcatagactt gtcggtaggt tctctgttgg cgctaaccgg cgcagttgct gcatctatcg
     8461 tcggcattga agtcaatgcg ctggtggctg tcgctgccgc tctcgcgtta ggtgccgcaa
     8521 ttggtgcggt aaccggagtg attgtagcga aaggtcgcgt ccaggcgttt atcgctacgc
     8581 tggttatgat gcttttactg cgcggcgtga ccatggttta taccaacggt agcccagtga
     8641 ataccggctt tactgagaac gccgatctgt ttggctggtt tggtattggt cgtccgctgg
     8701 gcgtaccgac gccagtctgg atcatgggga ttgtcttcct cgcggcctgg tacatgctgc
     8761 atcacacgcg tctggggcgt tacatctacg cgctgggcgg caacgaagcg gcaacgcgtc
     8821 tttctggtat caacgtcaat aaaatcaaaa tcatcgtcta ttctctttgt ggtctgctgg
     8881 catcactggc cgggatcatt gaagtggcgc gtctctcctc cgcacaaccc acggcgggga
     8941 ctggctatga gctggatgct attgctgcgg tggttctggg cggtacgagt ctggcgggcg
     9001 gaaaaggtcg cattgttggg acgttgatcg gcgcattaat tcttggcttc cttaataatg
     9061 gattgaattt gttaggtgtt tcctcctatt accagatgat cgtcaaagcg gtggtgattt
     9121 tgctggcggt gctggtagac aacaaaaagc agtaataacg actacaggac atcttgaata
     9181 tgaacatgaa aaaactggct accctggttt ccgctgttgc gctaagcgcc accgtcagtg
     9241 cgaatgcgat ggcaaaagac accatcgcgc tggtggtctc cacgcttaac aacccgttct
     9301 ttgtatcgct gaaagatggc gcgcagaaag aggcggataa acttggctat aacctggtgg
     9361 tgctggactc ccagaacaac ccggcgaaag agctggcgaa cgtgcaggac ttaaccgttc
     9421 gcggcacaaa aattctgctg attaacccga ccgactccga cgcagtgggt aatgctgtga
     9481 agatggctaa ccaggcgaac atcccggtta tcactcttga ccgccaggca acgaaaggtg
     9541 aagtggtgag ccacattgca tctgataacg tactgggcgg caaaatcgcc ggtgattaca
     9601 tcgcgaagaa agcgggtgaa ggtgccaaag ttatcgagct gcaaggcatt gctggtacat
     9661 ccgcagcccg tgaacgtggc gaaggcttcc agcaggccgt agctgctcac aagtttaatg
     9721 ttcttgccag ccagccagca gattttgatc gcaccaaagg tttgaacgta atgcagaacc
     9781 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     9841 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     9901 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     9961 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
    10021 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
    10081 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
    10141 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
    10201 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
    10261 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
    10321 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatct cctttattgc
    10381 ctgtactggc gatgacagca ttggcgagag cgttcgccag cagctcgcca ctgataacat
    10441 cgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
    10501 taatggcgaa ggtgagaatg tcattggtat tcatgccggc gctaatgctg ccctttcccc
    10561 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
    10621 ggagtcacca ctcaaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
    10681 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
    10741 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
    10801 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
    10861 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
    10921 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
    10981 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
    11041 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
    11101 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
    11161 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
    11221 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagctctggc
    11281 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
    11341 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
    11401 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
    11461 gatgcaaaaa cgcgttgatg gcttactgtt actgtgcacc gaaacgcatc agccttcgcg
    11521 tgaaatcatg caacgttatc cgacagtgcc taccgtgatg atggactggg ctccgttcga
    11581 tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag caacgcaata
    11641 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggcccgctgg ataaaactcc
    11701 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
    11761 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ctatgcgcca
    11821 actgctatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
    11881 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
    11941 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccatca
    12001 accaaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
    12061 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
    12121 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
    12181 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
    12241 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
    12301 aaactgatag acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
    12361 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa actgcgtcga catagccatc
    12421 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
    12481 aactgagcga tcattagccc aataatcacc gtgatcccca ctaatgtatg gcgatagccc
    12541 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
    12601 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
    12661 ataaggaacg gtacacagcc ggtccccaga cgggttgcaa tattgcctac gataccgatc
    12721 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gcgtcgtgca
    12781 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
    12841 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
    12901 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
    12961 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
    13021 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
    13081 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
    13141 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
    13201 aagacaacca gctgtggtag cgagttggaa agtgcgcagg ccagagaacc caacgtgaac
    13261 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
    13321 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
    13381 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
    13441 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
    13501 tttttatcgc tcattcaatg tcctgttatc gggttatcac ttatcaggtg agcgtagcag
    13561 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg ataatcgcat cgacaatcgc
    13621 ctgatgcaga tccagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
    13681 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
    13741 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
    13801 tgccgccatt tcggccatta acgtattgag atgcgctttc tgttctgcgg tgccaaccgt
    13861 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
    13921 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
    13981 ccagttcgat tgtggcatta cccgggtacc aattcgcggt cgcggtaaaa ccatcccttt
    14041 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    14101 cagctcaatc tcaccgggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    14161 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    14221 taaaggcatt actaatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    14281 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gcacattaac ggggcttttg
    14341 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    14401 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    14461 ttctcctgag aattccggca gagaaagcaa aaataaatgc ttgactctgt agcgggaaag
    14521 cgtattatgt acaccccgcg ccgctgagaa aaagcgaagc gg
//
LOCUS       DAZKFU020000115        14019 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZKFU020000115 DAZKFU020000000
VERSION     DAZKFU020000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.116"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(270..695)
                     /gene="arsC"
                     /locus_tag="ACHOQQ_RS13910"
                     /old_locus_tag="pgaptmp_002782"
     CDS             complement(270..695)
                     /gene="arsC"
                     /locus_tag="ACHOQQ_RS13910"
                     /old_locus_tag="pgaptmp_002782"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGU4471006.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDQL
                     VKLIADMGLTVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1028..1201)
                     /locus_tag="ACHOQQ_RS13915"
                     /old_locus_tag="pgaptmp_002783"
     CDS             complement(1028..1201)
                     /locus_tag="ACHOQQ_RS13915"
                     /old_locus_tag="pgaptmp_002783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471007.1"
                     /translation="MHPLTHPLPVTVHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHCQSENASELTG"
     gene            1229..1312
                     /gene="dinQ"
                     /locus_tag="ACHOQQ_RS13920"
                     /old_locus_tag="pgaptmp_002784"
     CDS             1229..1312
                     /gene="dinQ"
                     /locus_tag="ACHOQQ_RS13920"
                     /old_locus_tag="pgaptmp_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGU4471008.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1366..2718)
                     /gene="gorA"
                     /locus_tag="ACHOQQ_RS13925"
                     /old_locus_tag="pgaptmp_002785"
     CDS             complement(1366..2718)
                     /gene="gorA"
                     /locus_tag="ACHOQQ_RS13925"
                     /old_locus_tag="pgaptmp_002785"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGU4471009.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2790..3632)
                     /gene="rlmJ"
                     /locus_tag="ACHOQQ_RS13930"
                     /old_locus_tag="pgaptmp_002786"
     CDS             complement(2790..3632)
                     /gene="rlmJ"
                     /locus_tag="ACHOQQ_RS13930"
                     /old_locus_tag="pgaptmp_002786"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGU4471010.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3835..5877
                     /gene="prlC"
                     /locus_tag="ACHOQQ_RS13935"
                     /old_locus_tag="pgaptmp_002787"
     CDS             3835..5877
                     /gene="prlC"
                     /locus_tag="ACHOQQ_RS13935"
                     /old_locus_tag="pgaptmp_002787"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGU4471011.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5885..6637
                     /gene="rsmJ"
                     /locus_tag="ACHOQQ_RS13940"
                     /old_locus_tag="pgaptmp_002788"
     CDS             5885..6637
                     /gene="rsmJ"
                     /locus_tag="ACHOQQ_RS13940"
                     /old_locus_tag="pgaptmp_002788"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGU4471012.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6686..8155)
                     /gene="dtpB"
                     /locus_tag="ACHOQQ_RS13945"
                     /old_locus_tag="pgaptmp_002789"
     CDS             complement(6686..8155)
                     /gene="dtpB"
                     /locus_tag="ACHOQQ_RS13945"
                     /old_locus_tag="pgaptmp_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HGU4471013.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGSKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMVAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            8321..8374
                     /locus_tag="ACHOQQ_RS13950"
                     /old_locus_tag="pgaptmp_002790"
     CDS             8321..8374
                     /locus_tag="ACHOQQ_RS13950"
                     /old_locus_tag="pgaptmp_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471014.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            8352..8405
                     /locus_tag="ACHOQQ_RS13955"
                     /old_locus_tag="pgaptmp_002791"
     CDS             8352..8405
                     /locus_tag="ACHOQQ_RS13955"
                     /old_locus_tag="pgaptmp_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471015.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(8472..8906)
                     /gene="uspA"
                     /locus_tag="ACHOQQ_RS13960"
                     /old_locus_tag="pgaptmp_002792"
     CDS             complement(8472..8906)
                     /gene="uspA"
                     /locus_tag="ACHOQQ_RS13960"
                     /old_locus_tag="pgaptmp_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGU4471016.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9297..9632
                     /gene="uspB"
                     /locus_tag="ACHOQQ_RS13965"
                     /old_locus_tag="pgaptmp_002793"
     CDS             9297..9632
                     /gene="uspB"
                     /locus_tag="ACHOQQ_RS13965"
                     /old_locus_tag="pgaptmp_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGU4471017.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9703..11202)
                     /gene="pitA"
                     /locus_tag="ACHOQQ_RS13970"
                     /old_locus_tag="pgaptmp_002794"
     CDS             complement(9703..11202)
                     /gene="pitA"
                     /locus_tag="ACHOQQ_RS13970"
                     /old_locus_tag="pgaptmp_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGU4471018.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11434..12636
                     /gene="yhiN"
                     /locus_tag="ACHOQQ_RS13975"
                     /old_locus_tag="pgaptmp_002795"
     CDS             11434..12636
                     /gene="yhiN"
                     /locus_tag="ACHOQQ_RS13975"
                     /old_locus_tag="pgaptmp_002795"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGU4471019.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVEKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(12951..14003)
                     /gene="yhiM"
                     /locus_tag="ACHOQQ_RS13980"
                     /old_locus_tag="pgaptmp_002796"
     CDS             complement(12951..14003)
                     /gene="yhiM"
                     /locus_tag="ACHOQQ_RS13980"
                     /old_locus_tag="pgaptmp_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HGU4471020.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         3554 a         3562 c         3694 g         3209 t
ORIGIN      
        1 taatccagta cgtctttaaa ctgctgcact gtatcgcgga taactaagca gctcactgat
       61 acaccatgct ggacgcgata attggaatct cacgcaggga gtaatgtgta aatagcaaaa
      121 tcgcctgatg cgctacgcct gtcaggccta caaataacat actattcatc gaattttcat
      181 aactttgtag gtcggataag acgcgccagc gtcgcatccg acataaataa agcgcacttt
      241 accaactatc ggcagtggat atcgccgctt tatttcaacc gcttacccgc ttcatcaacc
      301 actttctcgc catcttcctt ggagaacgcg cctttttgcg catctggcag aatttccagc
      361 accacttccg aggggcggca taggcgagtt cccagcggcg tcaccacaat cggacggtta
      421 atcaggatag gatgttgcag cataaaatca attaaccgat cgtcagtaaa tttatcttct
      481 gcaaggccca gttcctcata cggctcgacg tttttacgca gcagcgcgcg tacggtaagc
      541 cccatatcgg caatgagttt gaccagttga tcgcgcgttg gcggtgtttc cagataatag
      601 ataatggtcg gttctgtacc gctgttgcgg atcatctcca gcgtattacg cgacgtgccg
      661 caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta caaagtgaaa
      721 gagagacgta gcgccagcgc agccagcgtt acaaaccaaa gcggataagt caaaaacata
      781 tatggtttaa cgaatgcgta tgtgcagagg aaggtaatag gtgtgaattt tgagttggct
      841 attcatttga aaggaggtcc gaatcaggac gtaaaaaaac cgactttgcg tcggtttttt
      901 actttccagc cctgagttgg tggctctggc tggagtgaga tagccatcat ctaacgcatc
      961 aatgctaata ccatactgaa actattgcaa ggacgtgctg gttttataac ctgcatgtac
     1021 tgtatgatta tccagttagc tctgaggcat tttcactctg gcaatgcgca taaacgcttt
     1081 caaagtcctg gtcagaagta cgggtggtgc cgttaactga tgctctggcc ggagtgagat
     1141 agtcgtcatc taacaatgag acatgcaccg tgacaggcag tggatgagta agcggatgca
     1201 ttctcactcc atcgcatgga gaaaacgggt gattgataaa gcaatcatcg ttcttggggc
     1261 gttaattgcg ctgctggaac tgatccgctt tctgcttcag cttctgaact gatagcggaa
     1321 acgtaattaa gggctaagag cacactactc ttagcccttt aacatttaac gcatggtcac
     1381 gaactcttct gccgccgttg ggtgaatggc gacggtattg tcgaagtctt ttttggttgc
     1441 ccccatcttc agcgccaccg cgaagccctg caacatttcg tccataccaa aaccaatgcc
     1501 gtgaataccg acaatcttct cttctggccc aacgcacacc agcttcatgc ggcacggctg
     1561 gcggtgagtg gtgacggcgg tatacatcgc ggtgaaagag gatttataca ctttcacctg
     1621 atcgtcgccg tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtac caatcggcgg
     1681 atggctgaag accacggtcg gaatgttgct gtaatccaga tgctcatccg gcttgttatt
     1741 aaacaggcgt tcagagaggc ggcgacccgc tgcaactgcc accggtgtca gttctactgc
     1801 acccgtgtta tcgcccaccg cgtaaatacc ttcaatgttg gtgttttgat atttatcgac
     1861 gacgatatag cctttttcgt tggttttaac gccagcggct tcgaggttga tgttgtcatt
     1921 ggcaggctcg cgaccaatcg cccaaatcag gcaatccacc gtttcactgc gaccatcttc
     1981 cagctccagc gtcaggctac catcggcatt tttcactacc gctttcggga tggcgttggt
     2041 gtgcaactgc gggccttcgg cgttcatcac ttcaaccagc gtttcagaga tcatcgggtc
     2101 gaagctgcgc agcggcgcat gtttacgcac aaacagatgc gttttcgcgc ccaggccgtt
     2161 aatcacgccc gccagctcaa cggcgatgta acccgcgcca acaaccgcca cgcgctctgg
     2221 caaagcagga agggcgaaga agccatcaga atcaataccg tattccacgc ccggaatatc
     2281 cgggtggctc ggacgaccgc cggtggcgat caggatatga tcggccgtga tggtttcgcc
     2341 gtttacttcc agcgttttgg catcaacgaa gcgggcaaag cctttgatta catcaacgtt
     2401 atttttaccg agcacgtttt cataggaagt atgaatacgg tcgatatagg cggtacggct
     2461 ggcgatcaac gtttcccagt tgaatttatt gatagtggta tcaaaaccat aatccgggcc
     2521 gtacatatgg atcgcttcac ggatttgcgc cgcgtgccac atcacttttt tcggcacgca
     2581 gccaacattc acgcaggtgc cgcccagctc tttggcttca atcagcgcgc atttctggcc
     2641 gtacatagcc gcgcggttga tggaggcgat accgccgctg ccgccgccga tggcgatgta
     2701 atcatagtgt ttagtcatga cagagtgtcc ttatcgttga ttaccgcgat tgtagcgcga
     2761 gacgtaatag gtgccagcaa tggctgcaat tactccggca cgatccagct tacggtggcg
     2821 tgcccggtgc cagccggaac cagtttgctg tgcagccacg gcagcacgtt attcatctgc
     2881 tgttccagct tccacggcgg gttaatcaca atcatgccgg aagcggtcat gccacggcga
     2941 tcgctgtctg gcagtaccgc cagttcaatt tgcagaattt tgcgaatacc ggtcgcttcc
     3001 agatcgtgga tcatgcgctt aatttgctga cgcagcacca ccggatacca cagtgcgtaa
     3061 gtaccagtgg cgaaacgttt gtaaccttct gctatcccgc tgaccaccgc ttgatagtcg
     3121 gttttcattt catacggcgg gtcgataagg attaaaccac ggcgggaaac cggcggcagt
     3181 ttggccttaa gctgctggaa accgtcggat ttttcgacgc gcgcacggct gtctttctga
     3241 aattcagaac gcagcaacgg gtaatcgctc gggtgcagtt cggtcagttg caggctgtcc
     3301 tgttcacgca gtaactgacg ggcaatcaac ggtgaaccgg ggtagtaacg caactgaccg
     3361 ctacggttga agtgttttac cacattgatg tacgcctcca gctccgcagg caaatcgtcc
     3421 tgctgccaga tacgggcgat accttcgaga tattcgccgg tacgctcggc atgttcgcta
     3481 cctaactgat aacgcccggc gcctgcgtgg gtgtcgagat agagaaacgg tttatctttc
     3541 tctttcagcg actcgatgat caggctctga acggtatgtt taagtacgtc ggcgtggttg
     3601 ccagcgtgaa agctgtggcg ataactgagc atgggtaaag gtgttccggt aagtaaaaat
     3661 cggcccgtat tcggggcgca caaaagccta tcaggacagt ataccgaaaa gaggccgccg
     3721 ccgcgaaagc gtaacgtttc tcattgaaat tcactacact taaccccatg ctacacacat
     3781 tatgtaaagc gcctgttgag cgcttcctta acctctttaa ccaggactgc gctaatgacg
     3841 aatccgttac tgactccctt tgaattgcct ccgttttcta aaattctccc ggaacatgtc
     3901 gttccagccg tgactaaggc attgaatgac tgccgcgaaa atgtggagcg cgtagtagcg
     3961 caaggagcac cgtacacctg ggaaaatctc tgccagccgt tggcggaagt ggacgatgtg
     4021 ttggggcgta ttttctcccc ggtcagccac ctgaactcgg tgaaaaatag cccggaattg
     4081 cgcgaagcgt acgaacaaac tctgccgctg ctgtcggaat acagcacctg ggtagggcaa
     4141 catgaagggc tgtataaagc gtaccgcgac ctgcgcgatg gcgatcatta cgccacgctg
     4201 aacacggctc agaaaaaagc ggttgataac gcactgcgcg attttgaact ctcaggcatc
     4261 ggtctgccga aagagaaaca acagcgttac ggcgaaattg ccactcgtct ttctgaactg
     4321 ggcaaccagt acagcaacaa cgtccttgat gcgacgatgg gctggaccaa actcgttacc
     4381 gacgaagcgg aactggcggg gatgccagaa agcgcgctgg ctgcggcaaa agcccaggcc
     4441 gaagcgaaag agctggaagg ctacctgttg acgctggata tcccaagcta cttgccggta
     4501 atgacctact gcgacaacca ggccttgcgt gaagagatgt atcgtgctta cagcacccgc
     4561 gcttcagatc aaggtccaaa cgccggtaaa tgggataaca gcaaggtgat ggaagagatc
     4621 ctcgctctgc gtcacgaact ggcgcaactg ctgggttttg aaaactacgc ctttaaatcc
     4681 cttgccacta aaatggcaga aaatccgcag caggtgcttg atttcttaac cgatctggca
     4741 aaacgcgcgc gtccgcaagg tgaaaaagag ctggcgcaac tgcgtgcctt cgctaaagcc
     4801 gaatttggcg tggatgagtt gcagccgtgg gatatcgcgt actacagcga aaaacaaaaa
     4861 cagcacctct acagcatcag cgacgagcag ctgcgtccgt acttcccgga aaacaaagcg
     4921 gttaacggcc tgtttgaagt ggtgaaacgt atttacggca tcaccgctaa agagcgtaaa
     4981 gatgttgatg tctggcatcc ggatgtacgt ttcttcgaac tgtatgacga gaacaacgaa
     5041 ctgcgcggca gcttctacct cgacctgtat gcccgtgaaa acaagcgcgg cggggcgtgg
     5101 atggatgact gcgtaggcca gatgcgtaaa gccgacggtt cgctgcaaaa accggtcgcg
     5161 tatctgacct gtaacttcaa ccgcccggtg aatggtaaac cggcgctgtt tacccatgac
     5221 gaagtgatca ccctgttcca cgagttcggt catggtctgc accatatgct gacccgcatc
     5281 gaaaccgccg gtgtatccgg gatcagtggg gtgccgtggg atgcggtcga actgccgagt
     5341 cagtttatgg aaaactggtg ctgggagccg gaggcgctgg cgtttatctc cggtcactat
     5401 gaaaccggcg aaccgttgcc gaaggagttg ctggataaaa tgctggcggc gaagaactac
     5461 caggcggcgc tgtttattct gcgccagctg gagttcggcc tgttcgattt ccgcctccat
     5521 gccgagttcc gcccggatca gggagcgaaa atcctcgaaa ctctggcaga aatcaagaaa
     5581 ctggttgccg tagtaccgtc tccatcctgg ggccgtttcc cgcacgcttt cagccatatt
     5641 ttcgccggtg gttatgccgc aggttattac agttacctgt gggccgacgt gctggcggca
     5701 gatgccttct cgcggtttga ggaagagggc attttcaacc gtgaaaccgg gcagtcgttc
     5761 ctcgacaaca ttctgagccg tggcggttca gaagagccga tggatctgtt caaacgcttc
     5821 cgtggtcgtg aaccgcaact ggatgcgatg ctggagcatt acggcattaa gggctgatca
     5881 ttcagtgaaa atctgcttaa ttgatgaaac aggcaccgga gacggtgcct tatctgttct
     5941 ggcggcccgc tgggggctgg agcacgatga agacaacctg atggcgctgg tgttaacgcc
     6001 ggaacatctg gaattgcgca agcgtgatga gccaaaactt ggcggcatct ttgttgattt
     6061 tgttggcgga gcgatggcgc accgacgcaa attcggcggt ggtcgcggtg aggcggtggc
     6121 gaaagcggtg ggcattaaag gcgattattt gccggatgtg gtggacgcta ccgcagggct
     6181 ggggcgcgat gcctttgtgc tggcttcggt cggctgccgc gtgcggatgc tggagcgtaa
     6241 tccagtcgtt gccgcgctac tcgacgacgg cctggcgcgt ggatatgcgg atgcggaaat
     6301 cggcggctgg ttgcaggagc gtttgcagtt aattcacgcc tccagcctga cggcgctgac
     6361 cgatattact ccgcgcccgc aggtggtcta tctcgacccg atgttcccgc ataagcagaa
     6421 aagcgcgctg gtgaaaaaag agatgcgtgt ctttcagtcg ctggtggggc cggatctcga
     6481 cgcggatggg ttgctggagc ctgcgcggct gctggcgact aaacgcgtgg tggtaaagcg
     6541 tccggactac gcgccaccgc tggcgaatgt cgccacgcca aacgcggtag tcactaaagg
     6601 gcatcgcttt gatatttatg ccggaacgcc ggtgtaaaag taaagcaggg tagcgataac
     6661 gctaccctgc tttctgcaag aataattaat ggctttccgg cgtcgcaatc atgcgtttca
     6721 gccacggcac catcaacagc atcactactg caacgcccag cgtgaccaga ccaatcttac
     6781 caaacacgtt ggtatagacg ggcaacgtct caagcggatc ggtaatgttg tccggcaccg
     6841 cggtaaatgt tgccacatag ccgcccagca agaacgcggc agcctgcgtc aggaaccaca
     6901 tcccgagaat aaagcccatc aaatgctgcg gcaccagagc ggcaaccatc gccagaccca
     6961 gggcgctaat aaacaattca cccaggctct ggaacaagta aaccagcacg ataaaccacg
     7021 gcgatgtcag tccttgtgca tcagcaaacc acattcccgc agcggcggcg gtaagaaagc
     7081 ccagcgagca cataaacatg ccgagagtaa atttcatcgg catagagaga tctttgcctt
     7141 tgctgcccag atgcgtgtaa atgcctgcca gtattgggct ggcgagtacc acccagaacg
     7201 ggttaagcgc ctggaagctg accgggttga tggaaaagcc gagaatttca tgatgcacgt
     7261 tgttgatggc aaagaagttc agcgacgttg gcatctgggc gtagagaatg taaaacacca
     7321 ccgcttcgag catcaggaca aaggcgacaa acattttatt gcgcccggtt ttatccagct
     7381 tgaatgcctg acgaaagaag atgatggtga cgacgatgga gagaacaatc agcaccagat
     7441 tggcgacttc tacgttgtgc atcagccatg cacagacgaa gatcatcacc acgctgccaa
     7501 gtaacacgta caacagtttg ctgaagctca tcggccggaa gtcgggttca gaaccaatgt
     7561 ctttcaccat tccacgacag gcgatgtaaa ccagtaatgc gataattaac cccgccccgc
     7621 acaggttgta ggtgacagaa taaccgaatc tatcagcgat cacaggggcc agcgataacg
     7681 ctatcaacga gccgatgttg atcgacatat agaacagggt gaatgcgcca tcaaggcgcg
     7741 gatctttcgg cggatagcac ttcgaaagca agctggctgg gttagcttta aacaggccgt
     7801 taccgacagc gatagtcccc agggcgatga aaatcaggtc aggcttaagt agcgacaggc
     7861 cggtcatgaa gtagccaatc gccagcacaa gtgctccgag aacaatggtg cgtttggtcc
     7921 ccagcaggtg gtcgccgaca tagccgccaa tggaaatgag gccatagacc agcgcagcaa
     7981 aagcaccaaa agtgacaaaa gcctgctctt gcgagaatcc aagctgttta acgaagaaaa
     8041 ccgccagtac gccctgcacg ccgtagtagc cgaatcgctc ccataactcg acaaaaaaga
     8101 tcatgaaaaa tgggcgaggt tgctgcagca tccccatggg tgttgttgta ttcatattta
     8161 ttaaccttcc aataccatcc tgaaaagtat gaacgcgtaa gggataacgc gcgaaaaagt
     8221 ttacatctgt cagagattct taatattgat gcggtgaata gattaataca ctattacaac
     8281 agaaaataac cagctgatca cactaatgat ggaaaatatg atggtgttaa atattgtatt
     8341 ggctggggat tttgattgcg aacgcagacc gtaggccaga taaggcgttt acgctgatca
     8401 ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag gaacacgttg
     8461 tggagggaag attattcttc ttcgtcgcgc agcggaacaa tcagcatatc aacgtgaacg
     8521 gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg gtggtgacca
     8581 caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg gcccaggtcg
     8641 ccgctgccgc tcagggtttc agtgattggg tatcctgcat tagtggaaag ctcggtcagc
     8701 gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac atcaataagc
     8761 ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt cgcattgtat
     8821 gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga gagatcgacc
     8881 gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg tcgatgactg
     8941 gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg
     9001 ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg acccggatga
     9061 tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga atatattgat
     9121 agtggttaac cttctggaaa aaaaacaacc tgatctccta cactatctat agagccgctc
     9181 ggatgttggt caccacaaaa gcggtttctc caggacctgg tgttaactga ttggcagcgt
     9241 atcgggagcg gtagccccgg ggtggaactc cgcgggcagg tcgccgggga ggaggtatga
     9301 taagcaccgt cgcattattt tgggctttat gtgtcgtttg cattgttaac atggcgcgct
     9361 atttctcatc actacgcgcg ttgttagtgg tactgcgtaa ctgcgatcca ttgctctatc
     9421 aatatgttga tggagggggc ttttttacct cacatggcca acccaacaaa caggtgcgtc
     9481 tcgtttggta tatctatgcc caacgttatc gcgatcatca cgatgatgag tttattcgcc
     9541 gctgtgagcg ggtgcgtcgg cagtttattc tgaccagcgc attgtgtggt ctggtggtgg
     9601 tcagcctgat tgcattgatg atttggcatt aagcacttca gcaataataa cgcataaaaa
     9661 aagcgggcca gtaaactgac ccgctcgttt tggtgtgtac gattacagga actgcaagga
     9721 gagccagtac agcccgccgg aaagcagtac cgcagccgga agggtaaaca cccaggccat
     9781 cagaatactg gttacggttt tacgctgtaa gccgccgcca tctaccacca tcgtccccgc
     9841 gacagaagag gagagtacgt gagtggtgga aaccggcatc ccggtataac tcgccaggcc
     9901 gatagacact gccgccgtca tctgggcaga catcccctga gcgtaggtca tgcctttctt
     9961 accgattttc tcaccgatag tcgttgccac acgacgccag ccaatcatcg taccgatacc
    10021 taacgccagc gcgaccgcca tgatgatcca caccggcgca tactcgatgg tgctaagcat
    10081 gtcggacttc agtttcttca acaggcgctg atcgtcagca ctcacaccag gcatcttcac
    10141 cactttgtcg atagtgtcag aaacgcacag cataatgcgg cgcatctggc tacgttgatc
    10201 aagcgacagc ttgtcgtagc tttccacatc ggtagtcaac atgcctttca ggcggttgag
    10261 cgcgttaatg gtattcgacg gatggcaatg aaactccgca ggttgcgttg cgccagcttc
    10321 cggagccggt actaactgat cagcaccggt agcttgtttc agcagcgcag gatgctgctc
    10381 aaagtaagct tcgacgttgt tgatggcatc acgggtacgg gtgatttcgt agccagtggc
    10441 attcatgttc accacgaagc ctgctggtgc gacaccaatc aataccaaca taaccagacc
    10501 aatgcctttc tgaccatcgt ttgcgccgtg cgaaaacgcc acgccgatag cggaaaggat
    10561 cagcgcgata cgcgtccaga acggcggctt tttcttgccg tctttctttt cacgctccgc
    10621 tggggtcagg tggatacggg cgcgtttctt ggtgccgctc cagtagcgac gcagcaagaa
    10681 aatcagaccg ccagcaaaca ccaggccgac aataggggaa acgatcagag aaccgaaaat
    10741 acttaatact ttcgggatat tgagtgcatc caccactgac gtcccggtca tcaacgcatt
    10801 ggttaaacca atcccgatga tcgcgccaat cagcgtatga gagctggatg caggtaaacc
    10861 aaagtaccag gtacccaggt tccagataat cgccgccagc aacatagaga acaccatggc
    10921 aaggccatga gacgatccca tattaagcag cagatccgtc ggcagcatat gcacaatagc
    10981 ataggcaaca ctcagaccac ccagcaaaac acccaaaaag ttgaacaccg ctgccataac
    11041 cacggcgagc tgagaacgca tcgcgcgggt atagataacg gttgccacgg cgttggctgt
    11101 gtcatggaaa ccattgatgg cttcgtagaa cagcacaaaa gccagtgcaa gcaataataa
    11161 cagcccggta tgcaaatcca ggccagcaaa caaatgtagc ataggacgtt acgccatttt
    11221 gaggacatga acgcggcgca ttatcagtga ctttcgcggc gcgggcaaag tgaaatatag
    11281 actttttttg atttgcctcc tgtatggatt tcactcaaaa aataattatc ttatataatt
    11341 caggcaaata cttcctttta gtaatattga tgctggtgcg accactgagg aatctttaca
    11401 attcacgccc gttttttcta agaggagcgc aacgtggaaa ggtttgatgc cattattata
    11461 ggcgctggtg cggcgggtat gttctgttct gcgctggcag gtcaggcagg acgccgggtt
    11521 ctgctgatcg ataatggtaa aaaaccaggg cgcaaaatcc ttatgtctgg cggtgggcgc
    11581 tgcaacttta ccaaccttta tgtcgaaccg ggcgcttatc tgagccagaa tccgcatttt
    11641 tgtaagtctg cactcgcgcg ttttacccag tgggatttca ttgatctggt caataaacac
    11701 ggcatcgcct ggcacgagaa aacgttaggg caactcttct gcgatgactc cgctcagcag
    11761 attgtcgaca tgctggtgga tgagtgcgag aagggcaatg tgaccttcag attgcgtagc
    11821 gaagtgctga gcgtggagaa ggatgataca ggcttcacgc ttgaactgaa cggcatgact
    11881 gtcggttgcg aaaagctggt catcgcgacc ggcgggctgt caatgccggg cctgggcgcg
    11941 tcgccgtttg gttataagat tgccgaacaa tttggcctca acgtgctgcc gacccgcgcg
    12001 ggtctggtgc cattcactct gcataaaccg ttgctcgaag agttacaggt gctggcaggc
    12061 gtggcggtgc cttccgtgat taccgctgaa aacggcaccg ttttccgaga gaacttactc
    12121 ttcacccacc gcggcttatc tggaccggcg gtgttgcaga tttccagcta ctggcaaccg
    12181 ggtgaatttg tcagtatcaa tctgctaccg gatgtggacc tcgaaacctt cctgaatgag
    12241 cagcgtaacg cacatccgaa tcagagcctg aaaaacacac tggcggttca tctaccgaag
    12301 cggttggttg aacgcttaca gcaactcggg caaatcccgg atgtctcgct aaaacagctc
    12361 aacgtgcgtg accaacaggc actgattagc acattgaccg actggcgcgt acaacccaac
    12421 ggcactgaag gctatcgcac tgccgaagtg acgctcggcg gcgtggacac taacgaactc
    12481 tcttcacgga cgatggaagc gcgcaaagtg cctgggctgt acttcatcgg cgaagtgatg
    12541 gacgtcaccg gctggctggg gggctataac ttccagtggg cgtggtcgag tgcgtgggct
    12601 tgtgcgcagg atttgattgc agcgaagtcg tcctgagctt tttcctaaag atgaaaatta
    12661 ttagagtcgc attaaaaatg agctaatttt gatagtggct atcttgtgat tattttctaa
    12721 tgagcccgtg aactgaaccc cccatgctta atataaggtg gagggaaagg tgattgaaaa
    12781 ctcacccagt gggctgatct ttataaaata tgaacaggtt gaaaaaaaac agtaactttg
    12841 ctgtttttta tacttaacca ctatgcatta atgctgcgtt atttcatgat acctaagaaa
    12901 aaccagcgtt acgcaaatgg tcaacgctgg ttttatccgg tacgttgcaa ttatttttta
    12961 gcagaacccg cttctaatat tgaaacgatt gagaacaacg taaagcatac cgctcccaaa
    13021 ccgaccagaa cccgcgaagg aataaaatat cccatatcgg tctgcgccat ttccgcaaga
    13081 aacgatgcca ggaaaaggca aaacaggcag gtgaagacgg gaatcatcgg tattctgttg
    13141 gctaacgaac aggtacgtct ccagaccagt gccagtagcc agacttttga gaatatgctg
    13201 taacagatca ttccgaggca aatgagaata atgccgggag ccagtcgggc gcttgcatcg
    13261 gagctgacta agacgtatat gccctgcagc accgtgattg agccgagaaa aataacccaa
    13321 taacaccaca gccagtgctc tttcgttgaa aacgtattac gtgtttgatg gacgattgtg
    13381 gcaacaaggc caattagaca ggcacagatt gcggttaacc cgagcaatac gtgaccggcg
    13441 acataatgcg gagttatgtc ggcactacgt aacagcgtaa tagaccaaat gaaaccgagc
    13501 agggtgagta acacgggaac ggcaatgagg caattaccga ttaatgaaga ataagcctgt
    13561 accggtgttc cgtcgctctt gctccccgct gcgtttttag gaatgagcag aaagtgaccg
    13621 gatgacgctg ccactgtcga tacacaggcg gcaatcatac caacgccgaa aataacatgg
    13681 ccggcgacaa actcgtctgc catcacatca ttacctgcta acagtgccca accccatatc
    13741 atggtgataa ttgaccccgc atagccaata atggggaaca atgtattgta aaaagtatta
    13801 acgccgcgcg tgagctgcga aatgataata aatgcggtag tgaataatgc caggcatatg
    13861 gctgccagag aaatgagcac atgacccgcg acgaaatact cgctttgccc tgaactttcc
    13921 agcacaaagc cacctaacgc gatacaaatc aagcccataa ttaaagggat taatttgaaa
    13981 agccacccga tatatatgtt cactttaaac tcctcaggt
//
LOCUS       DAZKFU020000116        14018 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZKFU020000116 DAZKFU020000000
VERSION     DAZKFU020000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.117"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            216..725
                     /gene="ybgA"
                     /locus_tag="ACHOQQ_RS13985"
                     /old_locus_tag="pgaptmp_002797"
     CDS             216..725
                     /gene="ybgA"
                     /locus_tag="ACHOQQ_RS13985"
                     /old_locus_tag="pgaptmp_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGU4471021.1"
                     /translation="MNLQRFDDSTLIRIFALQELHRLKERGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQNLDDYYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            722..2140
                     /gene="phrB"
                     /locus_tag="ACHOQQ_RS13990"
                     /old_locus_tag="pgaptmp_002798"
     CDS             722..2140
                     /gene="phrB"
                     /locus_tag="ACHOQQ_RS13990"
                     /old_locus_tag="pgaptmp_002798"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGU4471022.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVELVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLITYHPSLCKHCPFIAWTERVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2290..3771)
                     /gene="dtpD"
                     /locus_tag="ACHOQQ_RS13995"
                     /old_locus_tag="pgaptmp_002799"
     CDS             complement(2290..3771)
                     /gene="dtpD"
                     /locus_tag="ACHOQQ_RS13995"
                     /old_locus_tag="pgaptmp_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGU4471023.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            4042..4785
                     /gene="ybgI"
                     /locus_tag="ACHOQQ_RS14000"
                     /old_locus_tag="pgaptmp_002800"
     CDS             4042..4785
                     /gene="ybgI"
                     /locus_tag="ACHOQQ_RS14000"
                     /old_locus_tag="pgaptmp_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGU4471024.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4808..5464
                     /gene="pxpB"
                     /locus_tag="ACHOQQ_RS14005"
                     /old_locus_tag="pgaptmp_002801"
     CDS             4808..5464
                     /gene="pxpB"
                     /locus_tag="ACHOQQ_RS14005"
                     /old_locus_tag="pgaptmp_002801"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGU4471025.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5458..6390
                     /gene="pxpC"
                     /locus_tag="ACHOQQ_RS14010"
                     /old_locus_tag="pgaptmp_002802"
     CDS             5458..6390
                     /gene="pxpC"
                     /locus_tag="ACHOQQ_RS14010"
                     /old_locus_tag="pgaptmp_002802"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HGU4471026.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDQLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6380..7114
                     /gene="pxpA"
                     /locus_tag="ACHOQQ_RS14015"
                     /old_locus_tag="pgaptmp_002803"
     CDS             6380..7114
                     /gene="pxpA"
                     /locus_tag="ACHOQQ_RS14015"
                     /old_locus_tag="pgaptmp_002803"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGU4471027.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7150..7941
                     /gene="nei"
                     /locus_tag="ACHOQQ_RS14020"
                     /old_locus_tag="pgaptmp_002804"
     CDS             7150..7941
                     /gene="nei"
                     /locus_tag="ACHOQQ_RS14020"
                     /old_locus_tag="pgaptmp_002804"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HGU4471028.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7938..8984)
                     /gene="abrB"
                     /locus_tag="ACHOQQ_RS14025"
                     /old_locus_tag="pgaptmp_002805"
     CDS             complement(7938..8984)
                     /gene="abrB"
                     /locus_tag="ACHOQQ_RS14025"
                     /old_locus_tag="pgaptmp_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU4471029.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     VNLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9133..10194)
                     /gene="ybgO"
                     /locus_tag="ACHOQQ_RS14030"
                     /old_locus_tag="pgaptmp_002806"
     CDS             complement(9133..10194)
                     /gene="ybgO"
                     /locus_tag="ACHOQQ_RS14030"
                     /old_locus_tag="pgaptmp_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4471030.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     PLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     APIDVGPFDTVVLFKINYN"
     gene            complement(10191..10922)
                     /locus_tag="ACHOQQ_RS14035"
                     /old_locus_tag="pgaptmp_002807"
     CDS             complement(10191..10922)
                     /locus_tag="ACHOQQ_RS14035"
                     /old_locus_tag="pgaptmp_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4471031.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKAASQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKNPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10937..13396)
                     /locus_tag="ACHOQQ_RS14040"
                     /old_locus_tag="pgaptmp_002808"
     CDS             complement(10937..13396)
                     /locus_tag="ACHOQQ_RS14040"
                     /old_locus_tag="pgaptmp_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4471032.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALAAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWLGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITPLNLNLYANILHQSWWN
                     ADTSTTANITAGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNSTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSTGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13446..14012)
                     /locus_tag="ACHOQQ_RS14045"
                     /old_locus_tag="pgaptmp_002809"
     CDS             complement(13446..14012)
                     /locus_tag="ACHOQQ_RS14045"
                     /old_locus_tag="pgaptmp_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4471033.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGNGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVVLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
BASE COUNT         3411 a         3473 c         3818 g         3316 t
ORIGIN      
        1 aatataaagt cagcaataca acaaattata aaatataatt gatggtgaaa tatgcagcgg
       61 tactaccaat cggtcaagta ttcgctgctt tgatgatgat gtaaaatgat tgtttttgaa
      121 ctggatataa cagcttggta gggaagaaag cataacccca caaaattcat ttaccatcgt
      181 ctacacttac tcctgtaaac cgttcaggag cagtaatgaa tctacaacga tttgatgaca
      241 gcacccttat ccgcattttt gctttgcagg agttacaccg tcttaaagag cgaggcttaa
      301 cgcgcggggc gcttctcgat tatcacagcc gctataaact cgtctttctg gcgcattccc
      361 agccggaata ccgcaaactt ggcccgtttg tggctgatat tcatcggtgg caaaatctgg
      421 atgactatta caaccagtac taccaacgcg taattgtttt gctttctcat cctgccaacc
      481 cgcgagatca caccaacgtt ttgatgcacg ttcagggtta ttttcgcccg catattgatt
      541 ccacagaacg ccagcagctg gcagcgctta tcgacagtta tcgccgtggc gagcaaccgc
      601 ttcttgcgcc gctgatgcgt atcaaacact atatggcgct ttatcctgac gcctggcttt
      661 cagggcagcg ttatttcgaa ctttggccgc gtgtgattaa cttgcgccat tcaggagttt
      721 tatgactacc catctggtct ggtttcgcca ggatttacgt ctgcacgata atctcgcgct
      781 ggctgccgcc tgccgcaatt cgtctgcacg cgtgctggcg ttgtatatcg ctacaccacg
      841 ccagtgggcg gcgcataata tgtcgccgcg tcaggctgaa cttatcaatg ctcaactgaa
      901 cgggctacaa atcgcgctgg cggaaaaagg tattccttta ttgttccgtg aagtggatga
      961 ctttgtcgcc agcgtcgaat tagttaaaca ggtgtgcgcg gaaaacagcg ttactcatct
     1021 tttttataac tatcagtatg aggtgaatga gcgggcgcgg gatgtggacg ttgaaagagc
     1081 gctgcgtaac gtggtgtgtg aaggatttga tgacagcgtg atcctgccgc cgggggcggt
     1141 gatgactggc aatcacgaga tgtacaaagt ctttacgcct tttaagaatg cctggctgaa
     1201 acggctgcgg gaagggatgc cggagtgcgt cgctgcacca aaagttcgta gtagcggatc
     1261 gataaagccc gcgccatcca ttacgctgaa ttatcctcgt cagtctttcg atactgcgca
     1321 tttcccggtg gaagaaaaag cggcgattgc gcaattacgc cagttttgcc agaacggtgc
     1381 cggagagtat gagcaacaac gagattttcc ggcagtggaa ggcaccagcc gtttgtccgc
     1441 cagcctggca acgggcgggt tatcacctcg ccagtgtttg catcgcctgt tggcggaaca
     1501 gccgcaggcg ctggacggtg gggccggtag tgtctggctt aatgagctga tctggcgcga
     1561 gttttaccgt cacctgataa cgtatcaccc ctcgttgtgt aaacattgtc cgtttattgc
     1621 ctggacggag cgtgtgcagt ggcagagcaa tcccgcacat ttacaggcct ggcaggaagg
     1681 caaaacggga tacccgattg tcgatgctgc catgcgtcag cttaacagca ctggctggat
     1741 gcataacagg ctacggatga ttacagccag ttttctggtg aaagatttgt tgatcgactg
     1801 gcgcgaaggc gagcgatatt tcatgtcgca gctgattgat ggtgatttag cagccaataa
     1861 cggtggctgg cagtgggcgg cttctacggg tactgatgca gcgccgtatt ttcgtatttt
     1921 caacccgaca acccagggcg agaaatttga ccgcgaaggc gagtttatcc gccagtggct
     1981 accggaactg cgcaatgtac cggggaaatc ggtgcatgag ccgtggaagt gggctgagaa
     2041 agcaggtgtg aagctggatt atccgcaacc gatagtcgag cacaaagagg cgagagtaca
     2101 aacgttggca gcgtatgagg cggcgcggaa ggggaaataa cagacgcgtc aggcaatcga
     2161 gcccagatgc cggatgcggc gtgaacgcct tatccagcct acaaatttgt gcaaattcaa
     2221 taaattgcag gaaatacgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatcggt
     2281 gctggcctgt taagactcca gcgccagcgc gcggtttctg aatttcagcg cctgatacag
     2341 ccaaatcatc agtaccactc ctacacacgc cagtgcgccc caggtaattt gatcaaatac
     2401 ttcaatatat gcattgatgg agtagttgat cgccccggaa gcatcaaacg aagcctgcga
     2461 tgtctgatcg gcaatcacgc ccgccagata gttcgcaatc gcgccggaaa gcagcatata
     2521 aatgccggtt aatacgccgg tcacaccggg gatttcaatg cgcgtaatct gcgacatagc
     2581 aaccgggtcg ataaacagtt ccgcaaagcc catcaccgcc aggcctaata ccatcagtgg
     2641 cagagaagag tgaccataca ttgcggacca gcgggcgctt aaggtcagaa tgcagaatcc
     2701 ggcgctcatc aggccaaggc caagagcaaa ttttccccag atacgcacgg tacgattacc
     2761 cgcgacactc tcttttacca cccacgccag gaacacaccg cacagcataa ctgcgaaggc
     2821 attaatcgac tggaacatcg cggtcggaac ggtataacca aacatatcgc ggttaacgaa
     2881 gcggtcgata taaaggctaa tcgagctacc gccctgttgt gcgaaggccc agaacaacat
     2941 actgaagaag gtgagcgtca caatcagccc cagctcctta cgctgtttct ggttttctgc
     3001 tttgcgataa atttttgcca gtacacccag gccaatgata gtcgcgacaa tcaaggcgta
     3061 taccgaccat tctttccaga acagtacggt aatcagcgca ggcgttgcca ccagcagaac
     3121 cagcagccat ccccagttcg gcagcagaaa gtttgtcgca cgcagtactt ttttgttaac
     3181 gccgcgggta tgtgtgaaat gacgattgcc acataagaaa ataaccagac ccgcgatcat
     3241 acccaccgcc gccaggccaa agcccatcgc ccaactgtac tcttcctggg cgtagccaca
     3301 ggcaataggt gcgataatag accccacgtt acccgccgca tacatcagcg agaaaccgcc
     3361 atcacgacgc ggatcggttg gctcatacag ctcgccgagc aggcaactga cattggattt
     3421 aaacagacca tagccacaaa cgattatcgc cagggacaga tagaggaatg acggatggat
     3481 ctcacttgca cccaacacca catgaccgat cgccatcaat aacgccccca gcatcaccgc
     3541 catgcgattg ccgagaactt tatccgccaa aaagccaccg aggattggcg tgacatacac
     3601 cagcgaacag taggcgctaa ataactcgta ggcgtgagta tcgttgtatt ttagttgatt
     3661 ggtgagatag agaatcagca gggcacgcat gccgtaaaag ctaaaatatt cccagatttg
     3721 cagcgcaacg acatagtaaa tagcgcgcgg ttgtgatgcg tgtttattca taataattcc
     3781 acaattgacg gcaacgtcac gtaatagttg cgcggcgtgc ttgtgtttcc ttaaagttaa
     3841 aactttgata cagatctgat tatttatgca atatgctgtc tgattgcata aatatacatt
     3901 agctgaagcg tgatgatata aacaatttgc gtcttttggc gtcgggtttg tttcttaata
     3961 tgtcaaaaga ttggctatcg acgaacaggc gaggtaacgc tatgttaacg gtgtagctat
     4021 cagaatacgg aaggcgcgaa gatgaaaaac accgaactgg aacaactgat taacgaaaaa
     4081 ctgaacagcg cggcgattag tgattacgcg ccgaatggtt tgcaggtgga aggcaaagag
     4141 acggtgcaaa aaattgttac cggtgtcact gccagccagg ctttgctcga tgaggcagtg
     4201 cgtctgggcg cggatgcggt cattgtgcat catggctact tctggaaagg cgaatctccg
     4261 gtcattcgcg gcatgaagcg taaccgttta aaaacgttgc tggcgaatga tatcaacctg
     4321 tatggctggc atttgccgct tgacgcacat cctgagctgg gcaataacgc acagctggcg
     4381 gcgttactgg ggatcacggt catgggcgaa attgagccgt tggtgccgtg gggcgaactg
     4441 accatgcctg tgccgggact ggagctggct tcctggattg aagcgcgtct gggacgtaag
     4501 ccgttatggt gtggcgatac cggacctgag gtggtccagc gcgtcgcctg gtgcacgggc
     4561 ggcgggcaaa gttttatcga tagcgccgcg cgttttggcg tggatgcttt tattactggc
     4621 gaagtttctg aacagaccat tcattcagcc cgcgagcagg gattgcattt ttatgctgca
     4681 ggtcaccatg ccactgaacg tggtggtatt cgcgcattga gcgagtggct gaatgaaaat
     4741 accgatcttg atgtgacctt tattgatatt cctaatcctg cataacgaat aatcagaggg
     4801 atcgaaagtg caacgagcgc gttgttatct gataggtgaa acggcggtag tgctggaact
     4861 ggaaccgccg gtgacgctgg ctagccagaa acggatctgg cgactggcgc agcgtctggt
     4921 ggatatgccg aatgtggttg aagccattcc cggcatgaac aatatcacgg tgattttgcg
     4981 taatcctgag tcgctggcgc tggatgccat agagcgtttg caacgctggt gggaggagag
     5041 cgaggccctg gaacctgagt ctcgctttat tgaaattccg gtggtttacg gtggtgcagg
     5101 cggaccggat ttggcggtgg tcgcggcgca ttgcgggttg agcgaaaaac aggttgttga
     5161 attgcactcc tccgtggaat acgtggtctg gtttttaggt tttcaaccgg gcttcccgta
     5221 tctcgggagt ttgccggaac aactacacac gccacggcgc gctgaaccgc gcttgctcgt
     5281 tccggcaggt tctgtcggga tcggcgggcc gcagactggt gtttatccgc tggcaacgcc
     5341 gggtggctgg cagttgattg gtcatacctc actcagcctg tttgatccgg cgcgtgacga
     5401 acccatctta ttacgtccgg gagacagcgt gcgctttgta ccgcaaaagg agggagtatg
     5461 ctgaagatta ttcgtgcggg catgtatacc actgtgcaag atggcggtcg tcacggtttt
     5521 cgccagtcgg gtatcagcca ctgcggcgca ctggatatgc ctgcgttacg cattgctaac
     5581 ctgctggtgg gtaatgatgc caatgccccc gcgctggaga tcacgctcgg tcagttaacg
     5641 gttgagttcg aaactgatgg gtggtttgct ctgacgggtg ccggttgcga agcgcggctg
     5701 gatgataatg ccgtctggac cggctggcga ttgccgatga aagcaggcca gcgtttaacg
     5761 cttaaacgcc cgcagcacgg gatgcgcagt tatctggcgg tcgcgggtgg tattgatgtt
     5821 ccgccggtaa tggggtcatg cagcaccgat ctcaaagtgg ggattggcgg gctggaaggc
     5881 cgtttactga aggatggtga ccaactcccg attggcaaat cgaagcgtga ttttatggaa
     5941 gcgcagggcg ttaaacagct gctgtggggc aaccgcattc gcgccttgcc ggggccggaa
     6001 tatcatgagt tcgatcgcgc ctcgcaggat gcattctggc gttcgccctg gcagcttagc
     6061 ccgcaaagta accgcatggg ctatcgctta caggggcaaa ttttaaaacg caccaccgat
     6121 cgcgaactgt tatctcacgg tttgttaccg ggtgtggtgc aggtgccgca taacgggcag
     6181 cccattgtgt tgatgaacga cgcacagacc accggtggtt acccgcgtat tgcctgtatc
     6241 attgaggctg atatgtacca tctggcgcaa attccgctcg gtcagccgat tcattttgtc
     6301 cagtgttcac tggaagaggc actgaaagcg cggcaagatc agcaacgtta tttcgaacaa
     6361 ttagcgtggc ggctgcacaa tgaaaattga cctgaacgcc gatctgggcg aaggctgcgc
     6421 cagcgacgca gagctattaa cgctggtttc ctctgccaat attgcctgtg gatttcatgc
     6481 aggcgatgcg caaaccatgc aggcttgcgt gcgtgaagca ataaaaaatg gtgtcgcgat
     6541 tggcgctcat ccgagttttc ccgacaggga aaattttggt cgcagcgcta tgcagctgcc
     6601 gccagaaacc gtttacgccc agacgctgta tcaaattggc gcgctggcaa cgattgcccg
     6661 tgcgcaaggc ggcgtaatgc gtcatgtcaa accgcacggc atgttgtaca accaggcggc
     6721 gaaagaagca caactggcag acgccatcgc cagagcggta tacgcttgcg atccagcatt
     6781 ggttctcgtt gggctggcag gaagcgagct gattcgtgca ggcaagcaat atggcctgac
     6841 aacgcgcgag gaagtgtttg ccgatcgcgg ttatcaggct gacggctcgc tggtgccgcg
     6901 aagccagtca ggcgcgttga ttgaaaacga agaacaggcg ctggcgcaaa cgttggagat
     6961 ggtgcaacac ggcagagtca aaagtatcac cggcgaatgg gcaacggtca cggcgcaaac
     7021 ggtctgcctg catggcgacg gcgagcacgc actggcgttc gcccgccgac tacgctctgc
     7081 atttgccgaa aaggggattg ttgtcgcagc ataaccccga ttaataaaga atgaaaaaag
     7141 gatatcacca tgcctgaagg cccggagatc cgccgtgcag cggataacct ggaggcggcg
     7201 atcaaaggca aaccactaac tgatgtctgg tttgccttcc cgcagttaaa aacttatcaa
     7261 tcacaactta tcggtcaaca cgttacccat gtggaaacgc gtggtaaggc gttgttaact
     7321 catttttcca acgacttaac gctctacagc cataatcagc tttatggcgt ctggcgcgtg
     7381 gttgataccg gcgaagagtc gcagaccacg cgagtattgc gggtaaaact gcaaacggct
     7441 gacaaaacca ttctgcttta tagcgcctcg gatattgaga tgttgacccc ggaacaactg
     7501 accacgcatc cgtttttaca acgcgttggt cccgatgtgc tggatccgaa tctgacgccg
     7561 gaggtggtga aagaacggtt attgtcgccg cgctttcgta accgtcagtt tgctggatta
     7621 ctgctcgatc aggcgtttct ggctgggctt ggcaattatt tgcgtgtgga gatcctctgg
     7681 caggttgggt tgactggaaa tcataaagcg aaagacctca atgcggcgca actggatgca
     7741 ctcgcacacg cgttactgga tattcctcgg ttttcttacg ctacgcgggg gcaggtggat
     7801 gagaataagc atcatggggc gctgtttcgc tttaaggttt ttcatcgtga tggcgaactg
     7861 tgcgaacgct gtggcggcat cattgagaaa accacgctgt catctcgccc gttttactgg
     7921 tgtccgggct gccagcacta ggccgtacgt ttcggcgcat aggttgaaat aaaccgcgca
     7981 atggcaggcc ctgtcagcaa aatactgaac aggcgcaggg tttgcatcgc cataatgagc
     8041 gccatatcgg cattgctccc tgcggcgatg accgccacgg tatcaagccc gccggggctg
     8101 gtggcgaggt aggcagtcat aaaatcaatg tgcataaacc gggtcagccc ccacgccata
     8161 cccgcacaaa tagccagcag agcaaaaatc gacagcagga tttgtggtag cgggcgtagt
     8221 gcccgcagta agatttgttt atcgaaacca agaccaatcc gccagccaat tgccatatac
     8281 gccatcgcca gcagccattc cggtagttcg atggtgatga gctgaccaga ctggagcact
     8341 gcgcccgcca gcatcgggat gagcatcgtg ccggaaggaa gacgcagcag gcatcccact
     8401 gtaccggcaa cgaccgcgag caaaatagta agcaggagat taatgctcac tggcgggaac
     8461 cagacgatat gctggttaac cgcttcagcg ttatcgccca gcatcattcg cgtgaccaga
     8521 actgcggccc ccgcgacaaa cagcactcgc agatactgca taaacgccac caggcggata
     8581 tctgcgccgt aatcctgcgc catcgcgacc attgccgccg ccccgccagg cgaggagccc
     8641 caggcaccag tatttccggg cagtgagcta tagcgcacca gtagccatcc cacgatagcg
     8701 ctggaaagca acgtcaccag taaaatagct aacacaatcg gccagttaac cgccagcgtg
     8761 gtcaggatcg aaccggtcaa gttttgcgca atcatgcagc caagaatggc ctgcgcggcg
     8821 agaaaagcgg agcgggggag ttgcaggtta accccgcgca tactgaagat gatcccggcg
     8881 atcatcggcc caagtaataa tgccgccggg agatgtaccg cgaggaaacc aatagaaagg
     8941 agaagtgata gcacacataa cattccccac tgcaaaactg gcatcctttg ctccctcatt
     9001 atttactctt tggtgtatcg atacagcata aagaatgccg ggcatggaaa aaataacggt
     9061 aatcaataaa agccggaaaa ttggcaatta accgaagaag ccctctccgg ctggaaaggg
     9121 caataggcta aatcagttgt agttaatttt gaaaagtacc actgtatcaa agggaccaac
     9181 gtcgatgggc gcacccggcg tggatgacaa ttctgccgtg taggtttttt catacactaa
     9241 caggtcgctg gtaaaatcgg catactcact gtatttgttg aaagtatagg gtgaggcatt
     9301 ttcatccaac aatcgaagct tcaggccatt gccaatgagc agtgatttat cttcgtcgat
     9361 aaggggttca tcggtataaa acgaagagct gactttaaaa ccgtcggtac actggtcgtt
     9421 ttgtgatttg gttgtagtta ttttaaattg ctgcgacatc gtatggcgac gaatatccag
     9481 cacgttaaat cggccaaaat caacgacctg ggtttcaggt gagatagcaa agttgacgct
     9541 gcaatccagc acgcggatat tttccagacc ggtaatgtga tatttcagat tcttcgcggt
     9601 agggtcttcg ttcacgctgc ctgcgccatc aaactgtacg acgatgtaat cgctgagttt
     9661 gctctgataa tcatggggcg gcatggcgcg aactttaacg taaagccgca tacgcgccac
     9721 aaacgtccgc gatttgtgca taacggttgg atcgccggag caaagtttat tttgccagcc
     9781 catcgcaatg atctgctccg gggtgtaaat atcaatattc ttactgtcga tgcactgatt
     9841 cgtgtcgatg cggcttttcc caaggcttgc gtcatagtcc acgccttcat aggttacgcc
     9901 caattgataa tagggatcct gaataccggg gtaagggttt acccaggcgt agacgtgctc
     9961 gctttcaaaa ttaccgtttg tgttgttgtc acagtagacc ggaattttta tatcgtcaga
    10021 ttcccagatt ttatcgccgg gtttggaatt ggccggaacg gcgaagggca taatagcttc
    10081 cgatttttct acagcgccgt ttgcggtacc aaaatagcag ttcagcgcca gcgccgcttt
    10141 tagcggcaaa aaaatcagac agatggcgag caacacgcct ttggcgaatc tcatttttta
    10201 ttctccaccg gctgcaggcg gcattgccgc tggcaggaga gcgtgttcat acgcaatgcg
    10261 ccgtagtcgt ccatgtagcc aagataaaac tgcttgccgc tatagctgcc cgtgctggtg
    10321 gtgaccgaac tgaacggtgc gaccatcgtg cttttaaagc caggcagcac gcctttctca
    10381 tcccgcccaa gataggcaat agtcagatag tatccggtcg ggtttttcag ggtaagctgg
    10441 ttattctgct ggctaacctg tagctgttgc tcaacatgat cgcccatttt tttgcgtaat
    10501 gcggcgggtc gccagaatag cttaatgcga ctttggacgg cgacttgcag gatcgcatgg
    10561 ccctcgctgc cttccgggac aggcggaatt tcacgcaggt tatagtaaaa gagagattcg
    10621 cgatcggtgg gtaatttcgc ggtggcggct tgtttcatga tccgaacctg cgaagccgct
    10681 tttggctcca gtcgctggat aggcggcagg gcgacgagaa attcatcgct tttctctccc
    10741 ttttcatttt ctatccatga ataggcgagg taggggagtt tatcgctgcg gttttccacg
    10801 cgcaggctgg ttgctttatc tttgctatta aagatgatgc gcgtgcggtc aggctgcact
    10861 gcgccgtggc tgcacatact ggcaaccaga agaatcaaag ggaatccttt aacaaatgtc
    10921 atcgtctttt ccttaattaa tgacagggta aaatcaggcg cttagtcaca tcttccagat
    10981 gttccggcag atgaatggca cacgttttct ggtcgcccca gtgcacggta aattgttgat
    11041 tttcatcgac cccactcagc catgcgtgac cgttttcgcc aaccatcccg acgctgacac
    11101 cgccttcagc ctggcgaaca tcggcaccga gcggcgggaa atgcccgtta gcatcgctga
    11161 taatgacgtt gagatctttc ccggcgcggg acgccagcga tttaaagccg atggcgcctt
    11221 cggtccaggt ttctttcacc acgttttcgg aaacggtaac gccgtcaggc aagtcgttca
    11281 tattgaccgc caccgtcgta ggctggtagc tggaaacaaa cggtaccacg gcaataccaa
    11341 agcggttggt gtagtcaata ttgccctgaa tcggaatatc gcccacgccg tcggtgctga
    11401 ccatcaggcg tggttcattg gtggaactgc ggcgatggaa tgccgcgcca tgttgagtcg
    11461 cggtaaacga gccgctccaa ctggcactgg cggaagtgta atcattggcg gcataggttc
    11521 cggtcaaatc ccattccccc gtcgaactga ggtgttggta attgccgctg acctgcgcac
    11581 cgttatccgg gcgatcggat tgtattccag ccgacattcc ccaactgttg cgttcatcca
    11641 gggtatcgtt ccacgacatg cggtgggtgg tactattact gttgttctgc atgtcataac
    11701 ccagtcgacc actttcccca atcggtaagg agatcgaaaa gtaaatctgg ttgtcgcgat
    11761 ctttatcttc ataatgggtg gtgttgaacg aggtcgagac agaaatatct ttccagtcgc
    11821 caatgtcgac gttaaaaccg gcggtaatgt tggccgtggt ggaggtatcc gcattccacc
    11881 agctctgatg caggatgttg gcgtagagat taagattcag cggagtaata ggctgaccga
    11941 aggagaggct gatggtttgc ttttcatcct gcgcgtcggc gtcattgtat ttgtgatcaa
    12001 tgtagttagc gtagctgtga aaatggcgat cggagaagcg ataggctgcc aatgagatgg
    12061 tgctgttggt cgcatcaacc tgtttgctgt agttaaagcg gtagctgtaa ccctgctcat
    12121 cctgttgagt atcaaaatgg ctgtcggccc aggtcacgtc aaacgacagc gcccccagcc
    12181 agagcatatt ttgcccaata cccagcgcgc cggaacgata gtcatcgcct gcgagaagca
    12241 tgccgccata tagtgaagtg ttggagagca tcccccagga gacttcatgg ctaatgaagg
    12301 tttcatcctc ggtgtggtgc gacatagagg aacgcgggcg accggcagcc agtttatagc
    12361 gcacctggcc ctgacgcgtc aggaacgggg tagaggccgc tgaaacctgg aagttattca
    12421 cccgtccatc ttcttcgctc actttgacat ccagtgtacc ttgcactgac tgattcaggt
    12481 catcaataat aaatgggcct ggcggaactt ttttctggta aatcacgcga ccagaatggc
    12541 ttatcgtgac cgtggcgtta gtctgtgcga tgccgctgat ttgcggcgcg tagccgcgta
    12601 actcccaggg gagcattcga tcgtcgctgg ctaacgccgc accggtatag gagaaaccat
    12661 caaaaatgtt agagctaaaa tcggtttcgc ccagggttaa tcgggaacct atttgcggta
    12721 acgggcggaa caggtaggtc cgtgaaatcg ccccggattg ttcacggttg tcgccgctat
    12781 cggactggct aagctgataa tcgctgcgca gacgccatgc cccggcgttg aggcccgtgg
    12841 tgccataggc gttcaggttg ttgctgctgc tgccgctttg tgggcgataa gtgctggcaa
    12901 acaggttgta atccatcagg aagccaggaa taccgttatt ccacgttgag ggtggcgtcc
    12961 agttttctga atgccacgcc agccatgcct gcgggattgt aatattgagc tgttgatttg
    13021 cctgatcgaa agtaaagatg atttcaggga agacactaaa atcgacacat tcttttattg
    13081 ccggtaattt tttacgaaag tcagatttaa gtccgaagag ttcgatcagc gattcattga
    13141 tacaaggaat aattttatcg ccagattttt gccagcgaat ttgttgccca ttactgattt
    13201 tattattatt aaccgtaaca ctaacaaagt aatcgccggg agctacgacg cctttttgat
    13261 tgagcaacga tatatcaacg ctatcccgaa tcgacttatc gagaacgtta aggttaaatt
    13321 cagctgccag cgcaggaggg gttaccattg ccagacagga aagaacagag agtcgataaa
    13381 tattcacggc atccatacct gaacaatatt cgcaaaaggc ggctaatcag ccgccttaaa
    13441 aaggattatt ggtaatccag aacgtaagtt gcgttagcag tcacggcccc cgcggtaacc
    13501 gaaactgcgt tatccatctg ttccatccag gcaaagaagt ttaacgtttg ttctgttgaa
    13561 cttgccaata agtcaattgt tggcgcttct gttcccaata caacaaatga ttcatctttg
    13621 ttcatgagac gtacgccaac gccggtagcc tcgccagcac tgatgttact aaggagcggc
    13681 gttgcacctg tcgttttagc tgtgctgtcg aaggtcacgc ctacctttga gaccacgcca
    13741 ccgttgccat catcccagcc tgcaatatca cagttgatta agcgaatatt gacaggtttt
    13801 ttctcgctat gatgttctcg atttatgtgg gatgtggtta cttcacccag gtcaatgttc
    13861 atatcaaggt catcagggtg aatttcacaa ggtgcttcaa taacttcacc tttaaagtgg
    13921 atttcaccag atccttcatc tgcagcaaaa acaggtgaag cgaatagtag agatatggcc
    13981 agggcggcca gtgttttttg tcctttaaac atacatga
//
LOCUS       DAZKFU020000117        13906 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZKFU020000117 DAZKFU020000000
VERSION     DAZKFU020000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.118"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(139..885)
                     /gene="cutC"
                     /locus_tag="ACHOQQ_RS14050"
                     /old_locus_tag="pgaptmp_002810"
     CDS             complement(139..885)
                     /gene="cutC"
                     /locus_tag="ACHOQQ_RS14050"
                     /old_locus_tag="pgaptmp_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGU4471034.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(899..1465)
                     /gene="yecM"
                     /locus_tag="ACHOQQ_RS14055"
                     /old_locus_tag="pgaptmp_002811"
     CDS             complement(899..1465)
                     /gene="yecM"
                     /locus_tag="ACHOQQ_RS14055"
                     /old_locus_tag="pgaptmp_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4471035.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1681..3414
                     /gene="argS"
                     /locus_tag="ACHOQQ_RS14060"
                     /old_locus_tag="pgaptmp_002812"
     CDS             1681..3414
                     /gene="argS"
                     /locus_tag="ACHOQQ_RS14060"
                     /old_locus_tag="pgaptmp_002812"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGU4471036.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLATAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3456..3500)
                     /locus_tag="ACHOQQ_RS14065"
                     /old_locus_tag="pgaptmp_002813"
     CDS             complement(3456..3500)
                     /locus_tag="ACHOQQ_RS14065"
                     /old_locus_tag="pgaptmp_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471037.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3591..4079
                     /gene="yecT"
                     /locus_tag="ACHOQQ_RS14070"
                     /old_locus_tag="pgaptmp_002814"
     CDS             3591..4079
                     /gene="yecT"
                     /locus_tag="ACHOQQ_RS14070"
                     /old_locus_tag="pgaptmp_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGU4471038.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4201..4593)
                     /gene="flhe"
                     /locus_tag="ACHOQQ_RS14075"
                     /old_locus_tag="pgaptmp_002815"
     CDS             complement(4201..4593)
                     /gene="flhe"
                     /locus_tag="ACHOQQ_RS14075"
                     /old_locus_tag="pgaptmp_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGU4471039.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4593..6672)
                     /gene="flhA"
                     /locus_tag="ACHOQQ_RS14080"
                     /old_locus_tag="pgaptmp_002816"
                     /pseudo
     CDS             complement(4593..6672)
                     /gene="flhA"
                     /locus_tag="ACHOQQ_RS14080"
                     /old_locus_tag="pgaptmp_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(6665..7813)
                     /gene="flhB"
                     /locus_tag="ACHOQQ_RS14085"
                     /old_locus_tag="pgaptmp_002817"
     CDS             complement(6665..7813)
                     /gene="flhB"
                     /locus_tag="ACHOQQ_RS14085"
                     /old_locus_tag="pgaptmp_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGU4471040.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8015..8659)
                     /gene="cheZ"
                     /locus_tag="ACHOQQ_RS14090"
                     /old_locus_tag="pgaptmp_002818"
     CDS             complement(8015..8659)
                     /gene="cheZ"
                     /locus_tag="ACHOQQ_RS14090"
                     /old_locus_tag="pgaptmp_002818"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGU4471041.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8670..9059)
                     /gene="cheY"
                     /locus_tag="ACHOQQ_RS14095"
                     /old_locus_tag="pgaptmp_002819"
     CDS             complement(8670..9059)
                     /gene="cheY"
                     /locus_tag="ACHOQQ_RS14095"
                     /old_locus_tag="pgaptmp_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGU4471042.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9074..10123)
                     /gene="cheB"
                     /locus_tag="ACHOQQ_RS14100"
                     /old_locus_tag="pgaptmp_002820"
     CDS             complement(9074..10123)
                     /gene="cheB"
                     /locus_tag="ACHOQQ_RS14100"
                     /old_locus_tag="pgaptmp_002820"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGU4471043.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10126..10986)
                     /gene="cheR"
                     /locus_tag="ACHOQQ_RS14105"
                     /old_locus_tag="pgaptmp_002821"
     CDS             complement(10126..10986)
                     /gene="cheR"
                     /locus_tag="ACHOQQ_RS14105"
                     /old_locus_tag="pgaptmp_002821"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGU4471044.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSREWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11005..12606)
                     /gene="tap"
                     /locus_tag="ACHOQQ_RS14110"
                     /old_locus_tag="pgaptmp_002822"
     CDS             complement(11005..12606)
                     /gene="tap"
                     /locus_tag="ACHOQQ_RS14110"
                     /old_locus_tag="pgaptmp_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGU4471045.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQVEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12652..>13906)
                     /locus_tag="ACHOQQ_RS14115"
                     /old_locus_tag="pgaptmp_002823"
     CDS             complement(12652..>13906)
                     /locus_tag="ACHOQQ_RS14115"
                     /old_locus_tag="pgaptmp_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGU4471046.1"
                     /translation="LIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIV
                     TDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANT
                     LTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQ
                     ASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIAD
                     SSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAA
                     KEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQ
                     VALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPS
                     RPASEQSPAQPRLRIAEQDPNWETF"
BASE COUNT         3325 a         3905 c         3414 g         3262 t
ORIGIN      
        1 gcactgtcac ccgctcaaca gatcgcggta atcgttcatt ttttatacta ttcagggata
       61 aaaaaaggcc cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac
      121 atgatgcaac ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc
      181 aaccgccgcc ccgtctacga cataacgcga atactcgtcc gcgtgtgcat ctgatgacat
      241 ggacaatcct tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg
      301 gacttccagc actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc
      361 ggccataatg attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg
      421 cagtgcgtct gatttttgcc ctgatgtcag cacccgggca atgcccaatt ccgcaagatt
      481 attgagtgta tttagagggt tagcgcacat atcaaaggcg cgatgaaaag tcactgccag
      541 cggaccggca gcagccatta ttttttccat tcgtggcata tcgacattcc cgtcaacatc
      601 gagaacgccc gtcaccagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag
      661 aatggcggca aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg
      721 atgcacaggg atcgtcaccc gctggcgcac ggatttcagt acacccaacg acggcgttaa
      781 gcccccctct tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt
      841 tagtgcacat tccatgctgt aacagcaaat ttccagtaat gccattttta ctccttaatt
      901 acgccgactg ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg
      961 ttttgccatc ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca ccttttggcg
     1021 aactggtttt cactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg
     1081 cacgggcatt cagcgtttct ggatcacccg gcaaaacgat ttcaatatgt tcccaacctt
     1141 cgtgtgggta acgtttttcc ccgggccacg gtagctccac aatagaaaac tgccagtgcg
     1201 caacctgtac cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatatttt
     1261 ctgacaaaag ctcgccgcac tgttcaaacc cgcgacgcca gcgttcagcg gtggcgtttt
     1321 gatggcaacg caaggaaatg tgatcggcag tcaacggagt gatatccaac cccaaacggc
     1381 gggaaagttc atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct
     1441 cgtcgataga ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt
     1501 accctgttgc cctgcgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa
     1561 aacggcaata cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc
     1621 cctaatttct ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg
     1681 gtgaatattc aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg
     1741 cctgcggatt gcgaaccgca ggttcgtcag tcagcaaaag ttcagttcgg cgactatcag
     1801 gctaacggca tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag
     1861 caggtgctga ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca
     1921 ggctttatca acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg
     1981 gcgtccgatc gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt tgactactct
     2041 gcgccaaacg tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat tattggtgac
     2101 gcagcagtgc gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc
     2161 gactggggca ctcagttcgg tatgttgatt gcatggctgg aaaagcagca gcaggaaaac
     2221 gccggtgaaa tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac
     2281 gacgaagatg aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt
     2341 gacgaatatt tccgcgagat gtggcgcaaa ttggtcgaca tcaccatgac gcagaaccag
     2401 atcacttacg atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc
     2461 tacaacccaa tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa
     2521 agcgaagggg cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg
     2581 ggcgtgatca ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt
     2641 gcgaaatatc gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt
     2701 cagcatcaac acctgatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa
     2761 tccgtaccgc tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc
     2821 aaaacccgcg cgggtggtac agtgaaactg gccgatctgc tggatgaagc cctggaacgt
     2881 gcacgccgtc tggtggcaga aaagaacccg gatatgccag ccgacgagct ggaaaaactg
     2941 gctaacgcgg ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg
     3001 gactacatct tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg
     3061 cagtatgcat acacgcgtgt attgtccgtg ttccgtaaag cagaaattga cgaagagcaa
     3121 ctggctacag ctccggttat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg
     3181 ctgcagtttg aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt
     3241 gcttacctgt acgatctggc cggtctgttc tctggcttct acgagcactg cccgatcctc
     3301 agcgcagaaa acgaagaagt gcgtaacagc cgtctaaaac tggcacaact gacggcgaag
     3361 acgctgaagc tgggtctgga tacgctgggt attgagactg tagagcgtat gtaatcgatt
     3421 tttcgtgaga gtgaagcctg atcagggtta gccgatcagg cttttttatt gccatctaaa
     3481 tgtattctga aaacggacat gccatcgttt tctcactgtt gagtaagagg ccagaagcgt
     3541 aatatccagg cccaggaaaa cgataacggt tcaatttaag gaataccgca gtgtttaaat
     3601 ttcttgtatt aacattaggc attatctctt gccaggctta cgcagaagat acggttatag
     3661 taaatgatca tgacatttca gccatcaaag attgttggca aaaaaattca gatgatgata
     3721 ctgacattaa cgtgatcaaa tcatgtctgc gacaagaata caatctcgtc gatgcgcaat
     3781 taaataaagc ttatggtgaa gcttatcgtt atatagaaca agtgccacgc acgggtgcaa
     3841 aaaaacctga taccgaacaa cttaacttgc ttaaaaaatc acaacgagcc tggctggatt
     3901 ttagggacaa agaatgtgaa ttaatccttt caaatgagga tgttcaggat ttaagtgacc
     3961 cttattctga atcagaatgg ctatcatgta tgatcataca gaccaatacg cgaactcgcc
     4021 agttgcagct ataccgtaac tctgaagatt tttatccaag ccctttgaca agaggataat
     4081 tcacatcttt ttggcatgtt ttgttgcaag ctattcctga taaataattg caacaaaaca
     4141 tcgagccttt ttcgctgagt tattaaacat gctcgcgagc gcgtaatttt tatttgtcct
     4201 tcagcgataa ttcacaatca cttcattccg ttgtaccttt agcggtggaa ttaaccgccc
     4261 accgccaggc acttcccaga taaagcgcaa cggttctgct gccgctatac cggaaaaggc
     4321 cacggtggtt ccgctctgcc cctctaattc gacacaacga gattgcgaac acaagcgaac
     4381 ccgcagtcct gcaggtgtcg ggccgataag ctgataccgc cacgccacca gcgtcatcaa
     4441 tcctgaagca ggttgtcgag tagaaagagg cgctgacgac atcgactcac cgcgatgatt
     4501 tagcgtaatt ccgacactac ttgcctgcca catcccctcc ccggcggctt gcaccagcag
     4561 cggaaacaat aatattgcta ataaggttct catcatttac cgccaattgt cgccgtcatg
     4621 cggatatgtc ggttatcaga tagttccaga ttcgacagca ccactaactg cggcaagctg
     4681 cggcgcagaa agcgagacaa taatggtcgc agcgcgtggt tcaccaacaa tactggcggc
     4741 gcccccaaca tctcctgacg ggatagcgct tcctgagttt gcgccagtaa acgatccgcc
     4801 agccctggct ccagtccgcc cccgccctgc aacgcctgta gcaacaaacg ttccagcggt
     4861 gtatcgaggc caataacatg gacttcatct ttgccaggaa accactgctg ggtaatcgcc
     4921 cgtcccaacg ccacgcgcac gacggcggtt aattcatgtg gatcactttg gatgggtgca
     4981 tgttccgcca gcgtttcgag aatagtgcgc atatcgcgaa tcggcacttt ttcatcgagc
     5041 agattttgca gcactttatg cagtgtggtg agcgtgacga cgccaggaac gagatcttcc
     5101 gtcagctttg gcatctcctg ggcgacgcga tccagcagct gttgcgcctc ctgacgacca
     5161 aacagctctg cggcatgctg gctaatgagg tggttaagat gcgttgctac caccgtgctg
     5221 gcctcaacca ctgtgtaccc ctgaatctgc gcctgttctt ttagcgcact ttcaatccag
     5281 atagcattca ggccaaatgc cggatcgacg gtcgcctcac caggtaacgt cccggcagcg
     5341 gtaccagggt taattgccag ccagcgcccc ggataagcat caccactgcc aatctctacg
     5401 cctttcatca aaatacgata acgtgccggt tgcagatcca tattgtcgcg aatgtgcacc
     5461 accggcggca agaagcccat ttcctgagca aatttcttac gaatactacg aatacggccc
     5521 agcaactcgc catcttgttg gaaatcaacc atcgggatca gtcgataacc cacttccatt
     5581 cccagagaat cttccagttg tacatcgttc cacgtcgctt cgacaacggt attattctct
     5641 gccattttta ccggtttggg ttcggcaggc gctttttgtt cgcgtccgcg tatccaccag
     5701 gccagcccga gcaatccggc agtgaacagc aaaaatacca ggttcggcat tccaggcacc
     5761 aggccgagta aaccgagcac ggcggcgctt aacaacataa cgcttgggtt actgaaaagc
     5821 tgattcacca tctgctcgcc aacatcctga tcggtgctga cacgcgtaac gatgaccccc
     5881 gcggcggtag aaatcaccag cgccggaatt tgtgccacca gaccgtcgcc aatggtcaat
     5941 agcgtataac tttccgccgc gtgtcccatg ctcatgccat gttgcagcac gccgaccagc
     6001 aacccgccga caacgttaat gaccatgatg aggatcccgg cgatggcatc gccgcgaaca
     6061 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     6121 cgttttttcg cctcatcttc accaatcaat ccggcgttaa ggtcggcgtc aatcgccatc
     6181 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgcgat acgtccggca
     6241 cctttggtaa tgaccataaa gttgatgatc acgagaatga cgaacaccac gataccgata
     6301 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     6361 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     6421 aacagcgtgg taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     6481 aacatcgcca ccaggcaaca ccatgatcga caaggcaata ttgaaggtaa acagcaggtc
     6541 gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga tcaaaatcgg
     6601 tccggcaagg atctgccatt gtgtcgattt caggtttgtg ggcaggcgca gcatcgcggc
     6661 cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc accggaagat
     6721 gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt tgccagaccc
     6781 aggccagcac ttccgccacc gcggcgtaca gttgccccgg gatttgttga ccaatctccg
     6841 catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg ttattttcag
     6901 cgccgatttc acgaatgcgc agcgcgacaa gccctgcacc tttagcgacc actttcggtg
     6961 cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg ttattgacaa
     7021 tgacatccgc tttcggcaca tcggccatca tccgacgccg tgcggcagct cgctgcatct
     7081 gacggatccg tcctttaaca tgggggtcgc cttcgctttg tttgaactca tcacgaatat
     7141 cctgccgcga cattcgcagc tttttcaggt ggctgaagat ttggaaaaag acgtcaaatc
     7201 ccaccattgg aatgacacca agcaccacca gcagtgcgca tagccctacc aaatccatcg
     7261 cattacccat ggcggtaatc ggagactcgg ccattaagcg catcatctgc ggccagtgat
     7321 gccagagaaa aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt gctttaagca
     7381 actccgcgcc agtctgtgcc gagaacatcc gttttatgcc cggtagcggg ttgagtttgg
     7441 aaaacttcgg ctgcaaggat tttccgctaa ataccagccc ccccagcata accggagaaa
     7501 tgagcgccac cagcaccacg ccgctaatca gcggtagcag cgccagcatg gcttctctga
     7561 tcagcagaat aatttgcccg aggatcagat tcgggtcatt gataatactg tgatcaaaat
     7621 gcagcccagc ggagagcatg cccgacaatc gacgggccag cgacacaccg ccaaaccaga
     7681 taacactaac gcccaccagc aaaatcagca gtgaggtcag ttcacgggaa cgcgggattt
     7741 gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg ggcttctgtt ttgtcgtcgc
     7801 tctcgtcaga cacgtcgcca atcctggaaa gagtattaag cgtaaatgat gccagagcgc
     7861 aaagcgttca atggtttgag taaggggcaa aacaggcggg atttagggct tttgctgcca
     7921 cacatcaagc atagtgtgcg tttgtcggat gcggcgtcat cgccttatca gaccgcctga
     7981 tatgacgtgg tcacgccaca tcaggcaata caaatcaaaa tccaagacta tccaacaaat
     8041 cgtccacctg atcctgactg gctaccacac cggctttgct ggtatcgacc tgaggtccat
     8101 taagcaaact ctggttttca cgttttggac gcgactcctg ttccgggatg ttttccaaca
     8161 gcaccatcag caactggcgt tcgatctcct gaatgacatc catcatccgc ttaatgacct
     8221 gcccggtgag atcctgaaaa tcctgcgcca tcatgatttc cagcagttgc gcgttagtaa
     8281 agctggtatg cgcgggtaca tctgccagaa atttccgcgt atctgttacc agttcacggg
     8341 cgtcggcaag gtcaatcgga tcggcaaacc agtcatccca acgttgggtt aacgcttttg
     8401 ctgatttctc catttgatcc tgatgcggtt gtgacgcctc aacactgttc agcgcccgct
     8461 ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg atcgcgcgca tccgggatgg
     8521 cttccgccgc ttcggcaatg gcctgatcca gccccagttc ccgcaaactg tcgcgcagca
     8581 tacgcgtcag gctgccgatg cgcgcaatga tatcgccagc tgaatgctcg tcagcaggtt
     8641 tgattgatgg ttgcatcata gtcgcatcct cacatgccca gtttctcaaa gattttgttg
     8701 agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca catagccact ggcccccgct
     8761 tgcgccgcag caatgatgtt ctctttcttc gcttctgcag tcaccattaa cactggcaat
     8821 gctgacatcg cgccatccgc acgaattgtt ttcagcaatt ccaggccatc catgttgggc
     8881 atgttccagt cggaaataac aaatccataa ccgcctgcct gcaacttatt gagagcgtcg
     8941 acgccatctt ccgcttcctc aacattattg aatcccagct ctttcagcag gttacgcact
     9001 atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt taagttcttt atccgccatt
     9061 tcacactcct gatttaaata cgtatcgcct gtccggcact aatttttgcc agcatttgct
     9121 ggcttacctg gctaagatcg accacttcgc agacaccacc catattgatg gcctcacgcg
     9181 gcatgccgaa caccacgcaa cttgcttcgt tttgcgcaag ggtccatgcc cccgcctgac
     9241 gcatcgccaa cattcctgcc gcgccgtcgt tgcccatacc ggtcaggatc accccaaccg
     9301 cattacgccc cgcctgtttg gcgacagaat ggaacaacac atctaccgaa ggccgatgac
     9361 ggttaaccgc cgggccatcg tgaattttga tttgataatt tgcgccacta cgcgccagct
     9421 ccatatgccg atcgcccggc gcgatatagg catgccccgg caagacacgt tctccgtctt
     9481 cggcttcttt aaccccgatc tggcaaagct tattaagtct gtcggcaaaa gagcgggtga
     9541 aaccgggcgg catatgctgg gtaattaaca gtgccgggct ggaaagcggc aacggttgca
     9601 gtacgtgacg aattgcctca gttccacccg ttgaagcacc aatcgcaatc agtttttcag
     9661 aactcaacaa cggccctgcc ttcagcgttg tcggtgccga caatggctta tgtgctgcaa
     9721 ggctcgcctt tgctgctgta cgcacctttt cagcaatcat ttcgctgtac gccagcattc
     9781 cttcgcgaat acccagttgc ggtttggtga caaaatctat tgcccccagc tccagcgcgc
     9841 gcagcgtgac ttctgatcct ttgccggtca gggaagaaac catcacaacg ggcattggac
     9901 gcaaacgcat taatttttcg aggaaatcca gtccgtccat ccgcggcatt tcaacatcca
     9961 gcgtcagcac atcgggattg aatttcttaa tcaagtcacg cgcgaccagc ggatcaggcg
    10021 cggtcgccac catttccatg tcgctatggc tgttgatgat ttctgtcatg atctggcgca
    10081 tcagtgccga atcatcgaca gataacaccc tgattttgct catcgttaat ccttacttag
    10141 cgcatacacc gtctgaccac gcagcgtgaa gcggcgctca aggtggctaa agttttcaga
    10201 gtgacccgca aacagcaatc cgtcgggttt aaggagcgga acaaagcggc gcaaaatctc
    10261 ctgctgggta gtttgatcga agtagatcat gacgttacga cagaagatcg catcaaacgg
    10321 ccccggcacg gtgtactgtt tcgccagtag attcagcggg gcaaaatcaa catagttcgc
    10381 cagctcctga cgcacgcgta ccagcccttc atgcggtccc gtccctcgca tgaaataccg
    10441 ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg cgatagatac cgcttctggc
    10501 tttttccagc acttcggtgt cgatatcact ggcaaacact ttccagcgtc cgggcgcggt
    10561 gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg tcgaagccgc
    10621 cgcgctccat acgcgatact cgccagaacg gcgacgtgcg tgatccgcga gcagagggaa
    10681 atgatgtgcc tcacggaaaa atgccgtcag attcgtggtc agcgaattga taaacgcctg
    10741 ccactcacgg ctgtgctgat tagattccag caagttcaag taatgaccga aatccgtcag
    10801 tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt tatggtcagc
    10861 cagaacgatc ccggctcgtt gatagatcaa ttgacttatc cgccgaaaat gtgcgtcgga
    10921 aagcgccagg cgctcggtca tctgtaacaa taaagacgtt tgcccacagg gcagagatga
    10981 agtcatagcg ccttctcaat cacttcagga taccactggc gcaatttgta actgcgccga
    11041 ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc gccacacgcg acgaaagatg
    11101 gtcggcctga ttcgccagtt gttccgttgc caccgccgct tcttctacca gcgaggcgtt
    11161 ctgctgagtc acctgatcca tctggctgac agcctgtgca acctgctcaa tcccccgccg
    11221 ttgttcttcc gacgccgaag caatttctcc cataatgtcg ttcacgcggg tcaccgaact
    11281 gacaatatcg gtcatggtcg cggcggcgtt attcaccagt ttcgaaccct gctggacacg
    11341 attgactgac tcttcgatca gccctttgat ctcttttgct gcctgcgcgc tacggctggc
    11401 aagattgcgc acttcacctg ccactaccgc aaaaccacgc ccctgctctc cggcgcgagc
    11461 cgcttccact gccgcattca gggccagaat attggtctgg aaagcaattc cgtcgataac
    11521 gctgataatg tcgccaattt tttgcgagct ggtggcgatc tcctgcatgg tgtgagtcat
    11581 ggtactgacc tgaacaccgc ccgcctgcgc cgttgtcgca gcattttttg ccagttccga
    11641 cgcctgtcgt gcgttatcgg cgttttgccc taccgtggcg gttaattgct ccatactggc
    11701 agccgtttgt gccagcgatg ccgcctgctg ttcggtacga cttgagagat cgttattgcc
    11761 tgcgacaatc tctgcgatcc caatgtgcat ctcatggctt cccttacgca catcactcac
    11821 cgtcccacgc aaagcctgct gcatggtctt caggctggca aaaatggcgg tgatctcatt
    11881 acgaccatat accgcaatcg gacgcgccag attacccgca gcaatgctgt caaaatggct
    11941 accgataatg gccagtggtt gaacaatcat cttgcgcgtc caccacagcg cactgctgat
    12001 gtagatcgcc gcaacaataa tcatgctgat aaacaccagc gccgaaatct gatagttacg
    12061 ctggctttgc gcactggcgg cttccagcac atggttgatc tccacctgcc aggcctcaaa
    12121 gttaacgtca aacgcattct gtgatccctg caccggcgca gtgaggaaat ccgaaagttg
    12181 attactttca agccaggtcg cctgatgttc gagatcgttg tgccagcggg caaaactttt
    12241 ctccgtctct ttttgcaatg ccctgacgtg ctcgttgccc gcagtcatcg ccataaaact
    12301 tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc gtcgtcatca acgttttaat
    12361 gtcatccgcc ggatagctaa gtgccgtcag agtgcccgct ttgttcagcg cggtactggc
    12421 ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt tgctgattgc tctgctcgac
    12481 ctgattcagc cgttgcaaat cgtcgcgaaa tgcccaaaaa gacatgccgt tactgccaat
    12541 ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc gaatacgatt
    12601 aaacatcaac gctccccatc aggcggcaat gaccgcgtta gtaaatactc gtcaaaatgt
    12661 ttcccagttt ggatcttgtt cagcaattcg cagtcgtggc tgtgccggtg attgctcact
    12721 ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg ctggctgcca gacgaaacgc
    12781 ggaaaccgct tgcgttaaac gactagcctg ttcttccagc gcagctgcag cggcagctga
    12841 ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc atttccgaaa ccgccaatgc
    12901 gacttgatcg atgccacggc tctgttcatc cgatgccgat gcaatctcgc ccataatgtc
    12961 agtcacgcga gtgacagcat tgacgatatt gttcattgtt tccccggcgc tttcgaccag
    13021 caccgaaccg gtatcaacgc gcgagacgga gtcttcaatg agagctttga tctcttttgc
    13081 cgcctgggcg ctacgactgg caagattacg cacttcgccc gccaccacgg caaaaccacg
    13141 gccctgttca cccgctcgcg cggcttcaac cgcggcattc agcgcgagga tattagtctg
    13201 gaaggcaata ccgtcgatta cgctgataat gtcggcgatt ttcttcgaac tatcggcgat
    13261 ctcatgcatc gttttcacta cgccatccac cactttgccg ccgtgctggg cggtgtcgga
    13321 ggcactttgc gccagttgcg aggcctggcg ggcgttatcg gcgttttgct tcactgtcgc
    13381 ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg gatgcctgct gttccgtacg
    13441 ggaggaaaga tcggtgttac ccgccgcaat ttcacgggta ccggcataga tggcatctga
    13501 accttcgcgg acatgagtga cggtgtcagt caaagagcgt tgcatatgtg aaacgctctg
    13561 cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg gtattcgcca ggttaccacc
    13621 ggcgatttcg cgaatgtgag caataatttt tgccagcggt gtaagcaaca tacggcgaat
    13681 gccataccac gccaccagca gaatcaatac caccaccagc gcgataaccg ccagttgcca
    13741 ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata tcgcgataca gtttttcact
    13801 gctgagggcg tactgagcaa acgcttcgcc cattgcattt tgcattccct gggttggctg
    13861 agcgaaataa gctccagtat tgccataatc aagataatca atcagt
//
LOCUS       DAZKFU020000118        13890 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZKFU020000118 DAZKFU020000000
VERSION     DAZKFU020000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.119"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..1203
                     /gene="hmpA"
                     /locus_tag="ACHOQQ_RS14120"
                     /old_locus_tag="pgaptmp_002824"
     CDS             13..1203
                     /gene="hmpA"
                     /locus_tag="ACHOQQ_RS14120"
                     /old_locus_tag="pgaptmp_002824"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGU4471047.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(1248..1586)
                     /gene="glnB"
                     /locus_tag="ACHOQQ_RS14125"
                     /old_locus_tag="pgaptmp_002825"
     CDS             complement(1248..1586)
                     /gene="glnB"
                     /locus_tag="ACHOQQ_RS14125"
                     /old_locus_tag="pgaptmp_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGU4471048.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(1647..2981)
                     /gene="glrR"
                     /locus_tag="ACHOQQ_RS14130"
                     /old_locus_tag="pgaptmp_002826"
     CDS             complement(1647..2981)
                     /gene="glrR"
                     /locus_tag="ACHOQQ_RS14130"
                     /old_locus_tag="pgaptmp_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGU4471049.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEMFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMVRGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(2971..3684)
                     /gene="qseG"
                     /locus_tag="ACHOQQ_RS14135"
                     /old_locus_tag="pgaptmp_002827"
     CDS             complement(2971..3684)
                     /gene="qseG"
                     /locus_tag="ACHOQQ_RS14135"
                     /old_locus_tag="pgaptmp_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGU4471050.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDALRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(3849..5276)
                     /gene="qseE"
                     /locus_tag="ACHOQQ_RS14140"
                     /old_locus_tag="pgaptmp_002828"
     CDS             complement(3849..5276)
                     /gene="qseE"
                     /locus_tag="ACHOQQ_RS14140"
                     /old_locus_tag="pgaptmp_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGU4471051.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(5852..9739)
                     /gene="purL"
                     /locus_tag="ACHOQQ_RS14145"
                     /old_locus_tag="pgaptmp_002829"
                     /gene_synonym="purI"
     CDS             complement(5852..9739)
                     /gene="purL"
                     /locus_tag="ACHOQQ_RS14145"
                     /old_locus_tag="pgaptmp_002829"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGU4471052.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPVHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIYADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            9997..11553
                     /gene="mltF"
                     /locus_tag="ACHOQQ_RS14150"
                     /old_locus_tag="pgaptmp_002830"
     CDS             9997..11553
                     /gene="mltF"
                     /locus_tag="ACHOQQ_RS14150"
                     /old_locus_tag="pgaptmp_002830"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGU4471053.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQHMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(11550..12053)
                     /gene="tadA"
                     /locus_tag="ACHOQQ_RS14155"
                     /old_locus_tag="pgaptmp_002831"
     CDS             complement(11550..12053)
                     /gene="tadA"
                     /locus_tag="ACHOQQ_RS14155"
                     /old_locus_tag="pgaptmp_002831"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGU4471054.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(12111..12746)
                     /gene="yfhb"
                     /locus_tag="ACHOQQ_RS14160"
                     /old_locus_tag="pgaptmp_002832"
     CDS             complement(12111..12746)
                     /gene="yfhb"
                     /locus_tag="ACHOQQ_RS14160"
                     /old_locus_tag="pgaptmp_002832"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGU4471055.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            12955..13803
                     /gene="yfhH"
                     /locus_tag="ACHOQQ_RS14165"
                     /old_locus_tag="pgaptmp_002833"
     CDS             12955..13803
                     /gene="yfhH"
                     /locus_tag="ACHOQQ_RS14165"
                     /old_locus_tag="pgaptmp_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU4471056.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
BASE COUNT         3322 a         3794 c         3582 g         3192 t
ORIGIN      
        1 aaggaagacc atatgcttga cgctcaaacc atcgctacag taaaagccac catcccttta
       61 ctggtggaaa cggggccaaa gttaaccgcc catttctacg accgtatgtt tactcataac
      121 ccagaactca aagaaatttt taacatgagt aaccagcgta atggcgatca acgtgaagcc
      181 ctgtttaacg ctattgccgc ctacgccagt aatattgaaa acctgcctgc gctgctgcca
      241 gcggtagaaa aaatcgcgca gaagcacacc agtttccaga tcaaaccgga acagtacaac
      301 atcgtcggtg aacacctgtt ggcaacgctg gacgaaatgt tcagcccggg gcaggaagtg
      361 ctggacgcgt ggggtaaagc ctatggtgta ctggctaatg tatttatcaa tcgcgaggcg
      421 gaaatctata acgaaaacgc cagcaaagcc ggtggttggg aaggtactcg cgatttccgc
      481 attgtggcta aaacaccgcg cagcgcgctt atcaccagct tcgaactgga gccggtcgac
      541 ggtggcgcag tggcagaata ccgtccgggg caatatctcg gcgtctggct gaagccggaa
      601 ggtttcccgc atcaggaaat tcgtcagtac tctttgactc gcaaaccgga tggcaaaggc
      661 tatcgtattg cggtgaaacg cgaagagggt gggcaggtat ccaactggtt gcacaatcac
      721 gccaatgttg gcgatgtcgt gaaactggtc gctccggcag gtgatttctt tatggctgtc
      781 gcagatgaca caccagtgac gttaatctct gccggtgttg gtcaaacgcc aatgctggca
      841 atgctcgaca cgctggcaaa agcaggccac acagcacaag tgaactggtt ccatgcggca
      901 gaaaatggcg atgttcacgc ctttgccgat gaagttaagg aactggggca gtcactgccg
      961 cgctttaccg cgcacacctg gtatcgtcag ccgagcgaag ccgatcgcgc taaaggtcag
     1021 tttgatagcg aaggtctgat ggatttgagc aaactggaag gtgcgttcag cgatccgaca
     1081 atgcagttct atctctgcgg cccggttggc ttcatgcagt ttgccgcgaa acagttagtg
     1141 gatctgggcg tgaagcagga aaacattcat tacgaatgct ttggcccgca taaggtgctg
     1201 tgatttgatg ttgccggatg gaaacatccg gcaacccttg acgcggttta aattgccgcg
     1261 tcgtcctctt caccggtacg gatgcgaatg acccgtgcca cgtcaaagac gaagatttta
     1321 ccgtcaccga ttttgccggt ttgcgcagtg cgaataatgg tatcgacaca ggtatcgaca
     1381 atgtcgtccg gtacgacaat ctcaattttc actttcggca gaaaatccac catatactcc
     1441 gcgccgcggt acagctcggt atggcctttc tggcggccaa aacctttcac ctcagtgacc
     1501 gtcatgccgg taatgccgac ttcagccagt gcttcgcgga catcgtccag cttgaagggt
     1561 ttaataatcg catcaatctt tttcatgcta ttccttgaaa aggtcgcctg tcttttgatc
     1621 tgctaaacgt aacacataac gccaattcat tccttgaaat cgtttgcatc cagctcgtgt
     1681 cgggaaagca gtttataaaa ttctgtccgg ttgcgccccg ccattctcgc cgcatgggtg
     1741 acgttgcctt tggtaatttg cagcagctta cgcaaatagt tgagttcaaa ctgattacgt
     1801 gcctcaacaa aggttggcag cgccgtgttt tcgccctcca gcgcctgctc tactaatgca
     1861 tcactaatca ccggagatga ggtcagcgcc acgcactgtt caatcacgtt gaccaactgg
     1921 cgcacattac ccggccagct cgcggtcatc aggcgtttca tcgcatcggt agagaacgcg
     1981 cggacaaacg gtttatgtcg ctctgccgcc tggcgcaaca ggtgatttgc cagtagcgga
     2041 atgtcttctg tgcgctctgc cagagccgga attttcaggc tgacaacgtt aaggcggtaa
     2101 tagagatctt cacggaattc cccgcgcacc atcgcttttg gcaggtcgcg gtgagtggcg
     2161 gaaataatcc gcacgtcgat atcaatatcg cggttactgc ccagcgggcg cactttacgc
     2221 tcctgcaaca cgcgcagcag tttgacctgc aacggtgcgg gcatatcgcc aatctcatcg
     2281 agaaatagcg taccgccttc cgccgcctgg aataaacctt cgcgattgct gacagcgcca
     2341 gtaaacgcgc cacgcgcatg accaaacagc tccgactcca gcaattgctc gggcaatgcg
     2401 ccacagttaa tagcaataaa tggtttgcta ttgcgcgggc tggcgttgtg gatagcctgg
     2461 gcgaacatct ctttcccggt gccgctctga ccgttaatca aaacgctaac gtctgattgc
     2521 gccaccagcc gcgcctgttc cagcaaacgc agcatcagcg ggctgcgggt gacaatcgcc
     2581 tcgcgccagc gttcgtcggt ggctggcgcg gattgctcca gcgcatcatc aattgcctga
     2641 tatagcgcgt ctttatcgac aggcttggtg aggaaactaa aaacgccctg ctgtgttgca
     2701 gcaacggcat cgggaataga accatgcgcg gtaagaataa ttaccggcat tcccggctgc
     2761 actttctgga tttcagcaaa cagctgcata ccgtccattt catccatccg caggtcgctg
     2821 ataactaaat ctactttttc gcgattcagt acccgtaaac cttcagcgcc actttccgcc
     2881 gtgaccacac tgtagccttc gctggtcagg cgcaggccaa gcagtttcag caatcccgga
     2941 tcgtcatcga ccaataataa atgcgcaggt ttatggctca tcaggagtga cctcatgggt
     3001 ggatggcgcg ggcttttcac tctcgtgtgg cgtatccggc gagaaatttc cagccggttt
     3061 gcgggtcgag agctggcgtt caatatcagt caggttttcc agcttgcggg tggtgagttc
     3121 cagttgctgc tgtagaacgt gatgttgctg gcgcagagcg tccagctcgc tgtcgctgga
     3181 ctgctggagt ttactgtagc gttggcgctc ttccgccagt tgcagttgca gcgcctgacc
     3241 gtcgcgccag agttgataca gtgggcgaac ctgtgccggg atctcggtac ttaacgcttc
     3301 aatacgcgcg accagctggc ggcgctcata cggcgtaatt ttggcgtcgg cgagcaaaat
     3361 cccttgttta aaggtatttt gccagctgcc gtcgtcatat tggcgggctt gctgacgcga
     3421 ctgtgcaggc attaaacgat cagcacaatc catcgcccgc agccagtaaa gcggattggt
     3481 ttcagttgat ttaccttgca gcgcccagat gtcgctacat tcagtagaaa gatagtcagc
     3541 cagttgataa accggaattt tttcttctgc tggcgtatca atggctggct tattgtgact
     3601 ctgcacgcaa cccagcaatg ccagacatgg cagccctgcc agccacagcc gtcggggcaa
     3661 taatcgttga aaaatgtgtc gcatattcac cagacttaaa gcctatccca gtgggcgtaa
     3721 ttgttgcaga cagtctggac atggacagcg cggagaaacc ggagcgtaca tgtcgtacgt
     3781 gaggatttcg agcactgccc ggggccgaac tgacaaataa aatagcctga tgggatagac
     3841 tgttagcatt atttcgtgtt tttcgacgac ggtaattcaa tgcggaaaca aacgtcttgc
     3901 ccgctctcgt cgaccagata cagttcccct tgcatacggc gaatacaatc cctggcaatg
     3961 cttaatccca gaccgctgcc cttcaccgcc ccttttcgct ggtggcttcc ctgaaaaaag
     4021 ggttcgaaga tcatggcgcg ttcctcttgc ggaatgggcg tgcctgtatt gatgacatca
     4081 atataaaccc gcgcgccatg taaactgctg cgaaggcaaa tgttaccgga ttcagccccg
     4141 tagtgcaccg cattggagta aagattatcc agtacgctca tcagcagcat tggctccgcc
     4201 aggcaagctg ttgctttgag atcgacgtcg gtatgcatca ttttagcccg tgcgggcagg
     4261 ctatgagcag aaaccactgt ctccaccagc ggtgctaatt caacattctc cagttccacc
     4321 gcactgtccg cctgtttacg gttgtaatca agcagttgtt cgatcagttt ttgcaaattg
     4381 cggctgctgc tatcaagaat gctcaccacc tctttttgct ctggcgtaag cggcccgaca
     4441 acctgatcag ccagtaattc agtgccctcg cgcatactcg ccagtggcgt ttttaattca
     4501 tgagataaat gtcttaaaaa ttgatggcgt tgggattcca gccatgacag gcgctcactt
     4561 aaccaaagaa tacgttgccc aaccgagcgt aactcgctcg gtccactgaa cgaaacgcta
     4621 ttgcccagag aacgcccttc ccccaaccga ttgatcatcc gttcaatatt tttcaccggc
     4681 ccgataatca tccgcgtgaa aagtagcacc atcaccagac tcaccagaaa tagcaccagc
     4741 gattgccaac caaaatattg cccacgttcg gcgatttcac gctgaagttg ctgcccacga
     4801 gagaacacca ctgtgcgcgt ggcctgcacc atttcggtat tggcactggc aaaggcttca
     4861 agacgcgcgg cggcggcagc atcgggacca ctgttgttgc actgaagttg ggccagattg
     4921 ttcaagtcct gacgcaatgc ctggtaaagt ttatcgtctg gcagtacgcc tgcgtgggca
     4981 tcgagcattt cgctgtaacg cttgcgctgg ctttgataaa ccttcgcaag cgtagggtca
     5041 tccagcacgc aatactgacg gtagctacgc tccatctcca gcgccgcgtt ggtcattgct
     5101 tcgctgcgcc gggcatcgat aagcgtggtg cggttgacca gcgccgcctg gtcgctaagc
     5161 gcattcaggc tttgccaggc ttgccatgcc agcaccaaca ggggcagcag aatcagcaaa
     5221 aatgccagca ttaccagttg tcgtaatgag cggggaaaaa cgggccagcg tttcaaggtg
     5281 ttactctcgt cagacgcgaa tagcctgatg ctaaccgagg ggaagttcag atacaacaaa
     5341 gccgggaatt acccggcttt gttatggaat aaggcggtgc ctaactcgac gtttcgcccg
     5401 atggttgata tagctacgct gatatcagaa gttggacggc aggcaccttg ttgtgcgtca
     5461 ttcgaatttt atgtagcacg tcccgaaggg gctgacataa gtcggtgaat gagccactga
     5521 ttactattat gcagtaactg tgccaataaa gaaaatagtt tggtaacgta ctgattatac
     5581 gttgctttga ggggtttatg gcttccccgc tatttgaatg taaatcagcc actgtgtctc
     5641 taaaaagaga caacttaaga aaaacttatt agattacaat aaaatcaatc atttaagtgt
     5701 cgccagaaag cgacacggta aaccacccat tgtcgtgatt tacagacaca aaaaagctcc
     5761 cgaagttggg agcttataat agcggttgta tttaatgccg gatgcggcgt gaatgcctca
     5821 tccgggctgc aaaaccaatg agtttgacgg cttaccccaa ctgcttacgt gcattgcgga
     5881 aaatgcgcat ccatgggcca tcctcgcccc agttttcagg atgccaggag ttgctgacag
     5941 tacggaaaac acgttccggg tgcggcatca taatggtgac tcgaccgctt tcagtcgtga
     6001 ccgccgtaat accgttcgga gaaccgttcg ggttagccgg gtaggtttca gtgactttgc
     6061 cgaagttatc gacatagcgc agtgccacca gccctttgct ttccagtgcc gccagatgcg
     6121 ccgcatcacg cacttccacg cgcccttcac catgagagac agcaatcggc atttgcgagc
     6181 ccaccatccc ctgcaacagc agcgacgggc tttgggtcac ttcaaccagg ctgaaacgcg
     6241 cttcaaagcg atcggaggta ttgcgcacaa aacgtggcca caactcacta cctgggatca
     6301 gttcacgcag attagacatc atctggcaac cgttacatac ccccagcgcc agcgtttgcg
     6361 gacggtggaa gaaggttgca aactcatcgc gtacacggtc attgaacagg atagacttcg
     6421 cccaaccttc accggcaccc agcacatcac cgtaggagaa accaccgcac gcgaccaggg
     6481 cgtggaaatc ctccaggccc gtgcgtccag ccagcaggtc gctcatatgc acgtcgatag
     6541 catcgaagcc tgcacggtgg aaagctgccg ccatttcaac atgcgagttc acgccctgct
     6601 cacgcagcac agcaactttc ggacgtgcgc cagtggcaat atacggtgct gccacatctt
     6661 cgttgatatc gaacgacagt tttacattca ggcccggatc ggcgtcgttt gatttcgcct
     6721 gatgctcctg atcggcacac tccgggttgt cacgcaggcg ctgcatctgc caggtagttt
     6781 ctgcccacca gacacgcaac gtagtgcggc tttcgctgaa tacagtctgc ccgttggcgg
     6841 taatcacaaa acggtcaccg gaaaccgcct gcccaacata atggacgcaa tcagcaagcc
     6901 catgctgtgc cagtacggac tcgaccgctt cacggtcagc ggcacgaacc tgaatgaccg
     6961 cacccagttc ttcgttaaac aacgccgcca ggcgatcgtc acccagagtg gcgatatccg
     7021 cgtaaatgcc gcaatgacca gcaaaggcca tttccgccag cgttaccagc agaccgccat
     7081 cagagcggtc gtgatacgcc agcagcttac gctgtgcaac cagcgcctga atcgcgtcat
     7141 agaagccttt cagttgcgcg acatcgcgta catctgccgg tttgtcgcca agctgacgat
     7201 aaacctgcgc cagcgccgtt gcgcccagcg cgttattgcc tttgcccaaa tcaatcagca
     7261 gcagtgcgtt atcttcggta gaaagctgcg gcgtgatggt gtgacgtaca tcttccacgc
     7321 gggcaaatgc agaaatcacc agcgacagcg gcgacgtcat ttcgcgctct tcgttacctt
     7381 cctgccagcg ggttttcatc gacatagagt ctttacccac cgggatcgtc aggcccagcg
     7441 ccggacaaag ctcttcgccc acggctttaa cggcttcata caggcccgca tcttcaccag
     7501 ggtggcctgc cgccgccatc cagttggcgg aaagtttaat gcgtttgata tcgccaatct
     7561 gtgttgcggc aatattggtt aacgcttcac cgaccgccag acgggcagag gcggcgaaat
     7621 ccagcagcgc aaccggcgca cgctcgccaa tcgccatcgc ttcaccgtag tagctgtcga
     7681 ggctggcggt agtgaccgcg cagttagcga ccggcacctg ccacggcccc accatctgat
     7741 cgcgcgccac catgccggtt acgctgcggt caccgatggt caccaggaaa gttttctccg
     7801 ccacggtcgg cagatgcagt acgcgtttca ccgcgtctgc aatggtgatc ccttcacgga
     7861 ccagcgcatc gcccttcgct ttcagcgttt gtacatcgcg ggtcatcttc ggcgttttac
     7921 caagcaggac gtccagcggc agatcgatcg gctgattatc aaaatgacga tcgtgcagag
     7981 aaagatgcag ttcttcggtc gcttcaccaa tcaccgcgta gggtgcgcgc tcacgcttac
     8041 acagttcgtc aaacagcggt aactgatcgg cagcaaccgc cagcacgtag cgttcctggg
     8101 attcgttaca ccagatttcc agcgggctca tgcccggttc gtcgctcagg atatcgcgca
     8161 gttcaaattt accgccgcgc ccgccgtcgc ttaccagttc cggcatcgcg ttagaaagac
     8221 cgcctgcacc aacgtcgtgg ataaacagga ttgggttggc atcaccaagc tgccagcaac
     8281 gatcgatcac ttcctggcag cgacgctcca tttccgggtt gtcgcgctgg acggaagcaa
     8341 agtcgaggtc ggcatcagac tgacctgacg ccatggaaga cgccgctccg ccgccaagac
     8401 cgatgttcat tgccgggccg ccgagaacga ccagcttcgc accgacgttg atctcgcctt
     8461 tttgtacgtg atcggcgcga atgttgccga tcccgcccgc cagcatgatc ggtttgtgat
     8521 aaccgcgcag ctcttcgccg ttgtggctgt tcactttttc ttcataagta cggaagtagc
     8581 cattcagtgc cggacgacca aattcgttgt taaacgccgc gccgcccagc gggccttcgg
     8641 tcatgatgtc cagcgcggtg acaatgcgct caggcttacc gaaatcttct tcccacggct
     8701 gttcgaagcc cggaatacgc aggttggaaa cggagaaacc gaccagacca gctttcggct
     8761 tcgcgccacg cccggtggcg ccttcatcgc ggatttcacc gccggaaccg gtcgccgcgc
     8821 ccggccacgg tgaaatcgcc gtcgggtggt tgtgggtttc aactttcatc aggatatgca
     8881 ccggttcctg atggaaatcg tagcggcccg tttcgtggtc ggcaaaataa cggcccactt
     8941 cagaaccttc cattacggcg gcgttatctt tataagcaga gagaacgtga tctggcgtgg
     9001 tttcgaaagt atttttgatc atcctgaaca gcgatttcgg ctgctgttca ccgtcgataa
     9061 tccagtcggc attaaaaatt ttgtggcggc agtgctcaga gttcgcctga gcgaacatat
     9121 acagctcgat gtcgttcggg ttacgaccaa gctttgtgaa agcatcctgc agatagtcaa
     9181 tttcatcttc cgccagagcc aggccaagac gcaggttagc gtcgatcagc gcctgacggc
     9241 cctgccccag caaatcaacg ctggtaaccg gagtcggttg atggtgggca aacaactgct
     9301 ctgcatcatc taaagcaaaa aagaccgttt ccatcatgcg gtcgtgcagt tcagcggtaa
     9361 cctgctgcca ttgttcattg gtcagcgtac cggcttctat atagtaagca acgccgcgct
     9421 caaggcggtt tacctgttgt agcccgcagt tatgggcaat atcggtcgct ttcgaagacc
     9481 agggagagat ggtgccagga cgcggggtca ccagcaggag tttgccttgc ggggcgtggc
     9541 tggcgagtgc cgggccatat ttcagcaggc gttcaagttg tgcgtgctca tcatcgttta
     9601 acggcgcatt gaggtcagca aaatggacat actcggcgta aatattgtga accgggagcc
     9661 tggcagcctg aaaacgtgcc agcagtttgt tgattcggaa tgccgacagt gcaggcgaac
     9721 cacgcagaat ttccatcata agtctctcgt cttctaagct ttcggtgtac ccaagggggg
     9781 gaaacgggcg tcattataaa gaatctgatg cgctgacgaa accgtttgcg tggaaataaa
     9841 atcaccatcg tgaattagca acgcgtgccg ccaatggctg taataagttg ccatctggcg
     9901 caggtttacg caaaatgccg ctcatttatg agtaaacctt tcactattat tacgtttttt
     9961 caagctggga cgcgcacgac acagagaatt aactaattga aaaaattaaa gattaattat
    10021 ctgttcatcg gcattctggc actgctgctc gcggtcgctc tctggccatc cattccctgg
    10081 tttggtaaag ccgacaaccg tatcgccgcc attcaagcgc ggggagagtt gcgtgtgagc
    10141 accattcata ctcccctgac ttataacgaa atcaacggga aaccttttgg cctggattac
    10201 gaactggcga aacagtttgc cgattacctc ggtgtaaaac tgaaagtgac cgtgcggcag
    10261 aatatcagcc agctgtttga cgacctcgat aatggtaacg ccgacctgct ggcggcagga
    10321 cttgtctata acagtgagcg ggtaaaaaat tatcagcctg gccctaccta ttattccgtg
    10381 tcacaacaac tggtttataa agtgggtcag tatcgcccac gtacgctggg caacctgacg
    10441 gcggagcaac tcaccgttgc accgggtcat gtggtggtta acgatctcca gaccctgaaa
    10501 gaaacaaaat tcccggaatt aagctggaag gtagacgaca aaaaaggctc tgcggaatta
    10561 atggaagatg tcatcgaagg aaaactcgat tacaccattg ctgattctgt cgccatcagc
    10621 ctgtttcagc gcgttcaccc ggagctcgcc gtagcgctcg atatcaccga tgaacaaccg
    10681 gtgacttggt ttagcccgtt agatggcgat aatacccttt ccgccgccct gctcgacttc
    10741 ttcaacgaaa tgaatgaaga cggtacgctg gcacgcattg aagagaaata cctggggcat
    10801 ggcgatgatt ttgattacgt cgatacgcgc acatttttac gcgccgtcga tgcggtactg
    10861 ccgcagttaa agcccctgtt tgagaaatac gccgaagaaa ttgactggcg tttgctggcc
    10921 gctattgctt atcaggaatc gcactgggat gcacaggcca cttcaccgac gggtgtgcgc
    10981 ggcatgatga tgttaaccaa aaataccgcg caaagcctcg gcattacgga tcgtaccgat
    11041 gccgaacaga gcatcagcgg tggcgtgcgt tatttgcagc atatgatgag taaagtgccg
    11101 gaaagtgtgc cggagaacga gcggatctgg tttgccctcg ctgcgtacaa tatgggctat
    11161 gcgcatatgc tggatgcccg cgccctgacg gcaaaaacca aagggaatcc tgacagttgg
    11221 gctgacgtaa aacagcgtct gcctttactt agccagaaac cctattacag caagctgact
    11281 tacggctacg ctcgtgggca tgaagcctac gcttatgtcg aaaatattcg taagtatcag
    11341 attagcctgg tgggttatct gcaagagaaa gagaagcagg ctacagaagc ggcgatgcaa
    11401 ctggcgcagg attatccggc ggtatcgcct acggagttgg gcaaagagaa atttcctttt
    11461 ctctcgtttc tttcccagtc gtcatcaaac tatttgaccc attctccctc tctgctgttt
    11521 tccaggaaag ggagtgaaga gaaacaaaat taatccgtcg aggattgcgc ttttttctgc
    11581 gctttaattt cctggcggcg catgcgaaag aagtcactga gcaacgccgc gcactcatcc
    11641 gccagtattc cttccgtaat ttccactcgg tgattcatac ccggatgatg cagcacatcc
    11701 attaaagatc ccgcagcgcc agttttcgcg tcacgcgcac caaagaccac gcgaccaatg
    11761 cgactgtgga tcatcgctcc ggcacacatt acacatggtt caagcgtgac atacaacgtg
    11821 gcgtcgatca gacgataatt ttgcatcacc agaccgccct gcctcagggc catgatttct
    11881 gcatgtgcgg tgggatcatg tcgaccaatc ggacggttcc agccttcgcc aataacacga
    11941 ttgttatgca ctaataccgc gccgaccggt acttcccgct catcccaggc acgcttcgcc
    12001 agcgtcattg catgacgcat ccagtattcg tggctaaatt cgacttcaga caaaaagaaa
    12061 actccggtta taaaagcgcg gcgcattata cacggacgct atgctttact ctattccagt
    12121 tgctggagtt caccgcgcgg ggttactcgc cagcgatgct ggcaaaagta aagcagcggg
    12181 ttgtcctgat tactgtcgct atagccactg tacagccgca gcggcgtgcc gattttgcgc
    12241 tccagttgcg cgaccttttc atgtcccaga caacgcatcg tcaataccca accaccatag
    12301 ccacgctgaa tttggctggc gataagatta actcgcggca gccagggcgt atcgaaataa
    12361 accgcttcaa ccagcggctg cggagagccg gtaatcaacc agatatcagc atcggaactt
    12421 aacaggtagg tggttaatcg ctcctgaacc agcggaaagg cggtaacatt gtcacgaaac
    12481 cagcgtacga aatcggcctg caacgcctgc aaacgcgctt cattgtgacc aaaagtgcat
    12541 ccccacagaa gcagactcat cggccagcgt gccgcacgac cttttatcaa taacgcaatg
    12601 gctataatcg gcaacaacgg caggacaagt aacgcattca gcggttggcg acgcagtaaa
    12661 tagcgcagaa aactgccgaa catatcctgc tgatgcaatg ttccatctaa atcaaaaaac
    12721 accacacgac gctcgtgagt tgccaaacca tactcctctg gactgaaaca tcctgattaa
    12781 ttttcgctac tacatagcct aacagataga tcatcacttt tccggcaaca atcgaccgca
    12841 tcatggctaa ctggaatttt taattcatgg caattagcgg caatggaata taaaattcac
    12901 tcgcgtgtgt ctcatattgc ctgttgtcgc cactatttca tcgcgaggaa agatatgaac
    12961 ggcttacttc gtatccgtca gcgttaccag gggcttgccc agagtgataa aaaactggcg
    13021 gattatctgc tgctacaacc tgatacggcg cgccatttaa gctctcagca actggccaac
    13081 gaagccggag tcagtcagtc cagcgtcgtg aagttcgcgc aaaaactcgg ctataaaggt
    13141 tttccggcgc ttaagttggc gttgagtgaa gcgctggcaa gccagccgga atcgccctca
    13201 gtgcccattc ataaccaaat ccgcggtgat gatccgttac ggctggtcgg cgaaaaactg
    13261 attaaagaaa atactgccgc tatgtacgca acgctaaacg ttaatagtga agagaaactg
    13321 catgaatgcg taacaatgtt gcgctctgcg cggcggataa ttctgaccgg tattggcgct
    13381 tcgggtctgg tggcgcaaaa ctttgcctgg aagctgatga agattggctt caatgctgcc
    13441 gcagtgcgcg atatgcatgc gctactcgct accgtacagg cgtcgtcccc tgacgatctg
    13501 ttattagcca tttcttacac cggtgtacga cgcgagttaa acctggcggc agatgagatg
    13561 ctgcgagtgg gcggaaaagt gctggcgatt accggcttta ctcccaatgc cctgcaacag
    13621 cgtgcttctc attgcctgta taccattgcc gaagaacaag cgacaaacag tgcttcaatc
    13681 tctgcttgtc acgcgcaggg aatgttaacg gatttgctgt tcattgcgct gattcagcag
    13741 gatctggaac tggcaccaga acgtattcgt catagtgaag cgctggtgaa aaaactggtc
    13801 tgagtaaaga atgcgcgtat aatgcccgcc cggtttgtgt tgttttgaga gtttccttat
    13861 ggcgctgtta atcactaaaa aatgcatcaa
//
LOCUS       DAZKFU020000119        13888 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZKFU020000119 DAZKFU020000000
VERSION     DAZKFU020000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.120"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..5411
                     /locus_tag="ACHOQQ_RS14170"
                     /old_locus_tag="pgaptmp_002834"
     CDS             <1..5411
                     /locus_tag="ACHOQQ_RS14170"
                     /old_locus_tag="pgaptmp_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HGU4471057.1"
                     /translation="YRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKFSRLT
                     LGVLLALSGSASGASLEVDNGQITNIDTDVAYDAYLVGWYGTGVLNILAGGNASLTTI
                     TTSVIGGNEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDGGYLR
                     LGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQANSN
                     GKVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATILEGGLITAENTIIGGNATGVGTLN
                     VQDQDSVITVRRLYNGYFGNGAVNISNNGLINSKDYSLVGVQDGSHGVVNVTDKGHWN
                     FLGTGEAFRYIYIGDAGDGELNVSSEGKVDSGIITAGMKETGTGNITVKDKNSVITNL
                     GTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYTTIGV
                     AGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSGIVNV
                     SNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGELNITT
                     GGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLNVEGG
                     EVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSDAGYQ
                     VDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMGSSDV
                     TIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFAGVAQ
                     LKDSTFTLERDNTAALTHAMLQSDIENTTSVNVGEQSIGGLAMNGGTLIFDTDIPAAT
                     LAEGYISVDTLVVGVSDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLNLLEH
                     DDNHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGVRLTT
                     APGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVRQVSL
                     SNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTGQMGS
                     TVLFKEGSLTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSVSPNAEVSLD
                     NTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVGQFNI
                     DTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDLTKLGTGILTLNND
                     SAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMSGGTLRV
                     AKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMN
                     VKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAK
                     GKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSL
                     FSHRLHDRLGEPQYTDSRLSQGSASSMWMRHVGGHERFRTGDGQLNTQANRYVLQLGG
                     DLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDSN
                     KTGAYVDCWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEGTLNT
                     WYVQPQAQITWMGVKDSDHNRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQRE
                     FQPYIEANWINNSKVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNVGVQL
                     GDKGYSDTQGMLGVKYSW"
     gene            5622..5888
                     /gene="hipB"
                     /locus_tag="ACHOQQ_RS14175"
                     /old_locus_tag="pgaptmp_002835"
     CDS             5622..5888
                     /gene="hipB"
                     /locus_tag="ACHOQQ_RS14175"
                     /old_locus_tag="pgaptmp_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HGU4471058.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5888..7210
                     /gene="hipA"
                     /locus_tag="ACHOQQ_RS14180"
                     /old_locus_tag="pgaptmp_002836"
     CDS             5888..7210
                     /gene="hipA"
                     /locus_tag="ACHOQQ_RS14180"
                     /old_locus_tag="pgaptmp_002836"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HGU4471059.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            7481..7678
                     /locus_tag="ACHOQQ_RS14185"
                     /old_locus_tag="pgaptmp_002837"
                     /pseudo
     CDS             7481..7678
                     /locus_tag="ACHOQQ_RS14185"
                     /old_locus_tag="pgaptmp_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7683..8000
                     /locus_tag="ACHOQQ_RS14190"
                     /old_locus_tag="pgaptmp_002838"
                     /pseudo
     CDS             7683..8000
                     /locus_tag="ACHOQQ_RS14190"
                     /old_locus_tag="pgaptmp_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            8361..9071
                     /locus_tag="ACHOQQ_RS14195"
                     /old_locus_tag="pgaptmp_002839"
     CDS             8361..9071
                     /locus_tag="ACHOQQ_RS14195"
                     /old_locus_tag="pgaptmp_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4471060.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKDEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9113..11764
                     /locus_tag="ACHOQQ_RS14200"
                     /old_locus_tag="pgaptmp_002840"
     CDS             9113..11764
                     /locus_tag="ACHOQQ_RS14200"
                     /old_locus_tag="pgaptmp_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4471061.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALSLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSECTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTNGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDIYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLRLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            11778..12308
                     /gene="ydeS"
                     /locus_tag="ACHOQQ_RS14205"
                     /old_locus_tag="pgaptmp_002841"
     CDS             11778..12308
                     /gene="ydeS"
                     /locus_tag="ACHOQQ_RS14205"
                     /old_locus_tag="pgaptmp_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4471062.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            12321..12824
                     /gene="ydeR"
                     /locus_tag="ACHOQQ_RS14210"
                     /old_locus_tag="pgaptmp_002842"
     CDS             12321..12824
                     /gene="ydeR"
                     /locus_tag="ACHOQQ_RS14210"
                     /old_locus_tag="pgaptmp_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4471063.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            12884..13798
                     /gene="ydeQ"
                     /locus_tag="ACHOQQ_RS14215"
                     /old_locus_tag="pgaptmp_002843"
     CDS             12884..13798
                     /gene="ydeQ"
                     /locus_tag="ACHOQQ_RS14215"
                     /old_locus_tag="pgaptmp_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4471064.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENISLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         3864 a         2995 c         3511 g         3518 t
ORIGIN      
        1 tctatcgcgt gatatggaat tgcactctac aggtatttca ggcctgctcg gaattaactc
       61 gcagggcagg taaaacatcg acggttaatt tgcgtaaatc ctctggactg acaacgaaat
      121 tcagtagatt gacgctgggt gttttgctgg cactaagcgg ttcagcgtct ggtgcgagtc
      181 tggaagttga taatggtcag attaccaata ttgatactga tgttgcttat gatgcctacc
      241 tggttggctg gtatggcact ggagtgctta atattttggc tggcggtaat gcctccttaa
      301 ccactattac taccagcgtc attggcggta atgaggactc agagggcacc gttaatgttt
      361 tgggtggcac ctggcgattg tatgatagcg gaaataatgc aaggccttta aatgtgggtc
      421 aatccggaac ggggacgctg aatattaaac agaaaggtca cgttgatgga ggctatttaa
      481 gactaggttc ctcgacagga ggcgtcggga cggtcaatgt tgagggagag gactcggttc
      541 tgacgaccga attattcgaa atagggagtt acggtacggg ctcattaaat attacggata
      601 agggttacgt cacgagttca atagtcgcca ttttaggata tcaagcgaac agtaatggta
      661 aggttgtcgt tgaaaagggt ggcgagtggc taataaaaaa taatgattcc tcaattgaat
      721 ttcaaattgg taatcaagga actggggagg cgactattct cgagggtggg ctgattacgg
      781 ctgaaaatac gattatcggt ggcaatgcca ccggtgtcgg aaccctgaat gtgcaggatc
      841 aagactctgt catcacggta cgcagactct ataatggata tttcggtaat ggcgcagtca
      901 atatttccaa taatggacta attaatagca aagattattc attggtgggc gttcaggacg
      961 gttcccacgg tgtcgtcaac gtgaccgata aagggcattg gaatttcctc ggaacaggcg
     1021 aagctttccg ctatatctat atcggtgatg ctggcgacgg tgaacttaat gtctcgagtg
     1081 aaggcaaagt agattcggga attatcactg cggggatgaa agaaacaggc acaggcaaca
     1141 ttactgttaa ggataagaac tccgttatca ctaatctcgg aactaatctt ggttatgacg
     1201 gccacggcga aatgaatatc agtaatgagg ggcttgttgt cagcaacgga ggaagttcac
     1261 tcggttatgg agaaaccggc gtcgggaatg tcagcatcac cacggggggg atgtgggagg
     1321 tcaataagaa tgtctatacc accattggtg ttgcgggtgt cggaaacctc aatattagcg
     1381 atggcggtaa gtttgtatcg caaaatatta cttttttggg cgataaagca agcggtatcg
     1441 gcacactgaa cctgatggat gcgacatcat cgttcgatac tgtgggtatc aatgtcggta
     1501 attttggtag cggtatcgta aatgtcagta atggtgccac ccttaattca acgggctatg
     1561 gatttatcgg aggaaatgcc tccggtaagg gaatagttaa tatttcaacg gatagtctct
     1621 ggaatttaaa gacgtcttct actaacgccc aattgctaca ggtcggtgta ttaggcacgg
     1681 gtgaactgaa tattaccacc ggaggtatag ttaaagcgcg tgatacacag atagctctca
     1741 atgacaaaag taagggcgac gtgagggtgg atgggcagaa ctctcttctt gaaacattca
     1801 atatgtacgt agggacatct ggtacgggta cgttaaccct gacgaatagc ggcacgctga
     1861 atgtcgaagg tggagaagtt tacttaggtg tttttgaacc tgctgtagga acgctaaaca
     1921 ttggtgcagc tcacggtgag gcggcggcag atgccgggta tataaccaac gcgacaaaag
     1981 tagagtttgg ctctggcgaa ggtgtttttg tctttaatca taccaataac agtgatgccg
     2041 gctaccaggt cgatatgctg attacaggtg acgataaaga cggaaaagtg atccatgatg
     2101 caggccatac ggtgttcaat gcagggaata cttatagcgg taaaacgctg gtcaatgacg
     2161 gcctcctgac cattgcgtct catacggcag atggggtaac gggcatgggt tcgagtgatg
     2221 taaccattgc aagccccggt acgctcgaca ttctcgcatc aacgaacagt gcaggagatt
     2281 acacgctgac caatgcgctc aaaggcgatg gcttgatgcg agttcagctg tcatcctccg
     2341 acaagatgtt tggctttacc catgcaacag ggactgaatt cgccggtgtt gcccaactga
     2401 aagacagtac cttcactctg gaacgcgaca acaccgctgc gcttactcac gcgatgttgc
     2461 agtctgacat tgaaaatacc acatcggtaa acgtgggaga gcaatccatt ggtggactgg
     2521 ccatgaatgg cggtacgctc attttcgata cggatattcc tgctgcgacg cttgcagagg
     2581 gatatatcag cgtcgatacg ctggttgtcg gcgtgagtga ctacacctgg aaaggccgta
     2641 actatcaggt aaacgggacg ggcgacgtgc ttatcgacgt gcctaaaccg tggaatgatc
     2701 ctatggcgaa taaccctctg acgacgctca atttgctgga acacgatgat aaccatgtcg
     2761 gcgttcaact ggtgaaggcg caaacggtta ttgggtcggg tggctcatta acgttacgtg
     2821 atttacaggg cgacgaggtg gaagcggaca aaacgttaca cattgcgcaa aacggaacgg
     2881 tggtcgccga gggtgattat ggagtccgcc tcacgaccgc accaggtgat ggtttgtacg
     2941 ttaactatgg gctgaaagcg ctgaacatcc atggagggca aaagctgacg ttagccgaac
     3001 atggcggagc ctatggcgca acggccgata tgtcggcaaa aatcggtggt gaaggggatc
     3061 tggcaatcaa tacggtgcga caggtttcgc tttccaacgg tcagaacgac tatcaggggg
     3121 caacctacgt tcagatgggg acattacgta ccgatgcgga tggcgcgctg ggcaacaccc
     3181 gggaactgaa catcagcaac gcagccatcg tcgatcttaa tggatcgacg cagacggtag
     3241 agacattcac cgggcagatg ggttcgactg ttttgttcaa agaagggtcg ctgacggtaa
     3301 ataaaggtgg gatcagtcag ggtgaactga caggtggcgg aaacctgaat gttacagggg
     3361 gaacgctggc tattgagggg cttaatgcac gctacaatgc gttaaccagc gttagcccaa
     3421 atgcggaagt cagcctcgat aatacgcagg ggttaggcag aggaaatatt gccaatgacg
     3481 gtctgttaac gctaaaaaac gtgactggcg aactgcgtaa tagcataagc gggaagggta
     3541 tcgtgagcgc aaccgccagg acagatgtag agctggatgg cgataatagc cgctttgtgg
     3601 ggcaattcaa cattgataca ggcagcgcgc tcagcgtcaa cgagcagaaa aacctgggtg
     3661 atgcttccgt tatcaataat ggcctgctca ccatctccac tgagcgtagc tgggcgatga
     3721 cgcacagtat cagcggtagc ggtgatttga caaaactggg taccgggatc ctgactctta
     3781 acaacgattc cgcggcgtat cagggtacga cggatatcgt gggtggtgaa attgctttcg
     3841 gttccgactc tgccattaat atggcaagtc agcacattaa tatccataac agcggtgtga
     3901 tgtcgggaaa tgtcaccact gcaggtgatg tgaacgttat gtctgggggg acactgcgtg
     3961 tcgctaaaac cacaatcggc ggcaacctgg agaatggcgg cacggttcaa atgaacagtg
     4021 aaggaggaaa accggggaat gtactgaccg ttaacggcaa ctataccgga aacaatggcc
     4081 tgatgacgtt caacgcgacg ctgggcggcg ataattcacc caccgataag atgaacgtga
     4141 aaggcgatac ccaagggaac actcgcgttc gggttgataa cattggcggc gtcggtgcgc
     4201 aaacggtcaa cggtattgaa ctcattgagg ttggcggtaa ttctgcaggt aacttcgcgt
     4261 tgaccaccgg aactgtcgaa gctggggctt acgtctacac gctggctaaa gggaagggga
     4321 atgacgagaa aaactggtat ctgaccagta aatgggacgg cgtaacgcca gcggatacac
     4381 ccgatcccat caataatccc cctgttgtgg atccggaagg cccatcagtt tatcgcccgg
     4441 aggccggaag ctatatcagc aacattgccg cagccaactc gctgtttagc catcgcttac
     4501 acgaccgtct gggtgagccg cagtatacag attcacggct ttctcagggt tcggcaagca
     4561 gtatgtggat gcgtcatgtc gggggacacg aacgtttcag gaccggtgac ggccagctaa
     4621 atactcaggc taaccgctat gtattgcagc taggcggcga tttggcgcag tggagtagca
     4681 acgcgcagga tcgctggcat cttggcgtga tggcaggcta cgccaatcag cacagtaata
     4741 ctcagagtaa tcgtgtgggt tataaatcgg atgggcgcat cagcggttac agcgctgggc
     4801 tgtacgcgac ctggtatcag aacgattcga ataagaccgg cgcttatgtt gactgctggg
     4861 cgctgtataa ctggtttgat aacagcgtca gttccgataa ccgttctgct gacgactatg
     4921 attctcgcgg tgttacggcc tctgttgagg gtgggtatac ctttgaagcg ggaacattta
     4981 gcggcagcga agggacgctg aatacctggt acgtccagcc acaggcgcaa atcacctgga
     5041 tgggtgtgaa agattctgac cataaccgga aagacggaac acgcattgaa acggaaggcg
     5101 acggaaacgt gcaaacgcga cttggggtga aaacctacct gaatagccat caccagcgtg
     5161 acgatggtaa acagcgtgag ttccagcctt acattgaagc gaactggatc aacaatagca
     5221 aagtctacgc cgtgaagatg aatggtcaaa ccgtaagccg tgatggtgcg cgtaatctcg
     5281 gtgaagtacg taccggggtt gaggcgaaag taaataacaa ccttagcctg tgggggaatg
     5341 tcggtgtgca actaggtgat aaaggctata gcgatactca gggcatgctg ggagtgaaat
     5401 atagctggta aacagtataa gccgcatgtc gagatggcat gcggcttaat attgccaact
     5461 tcaaaaggct tttaaagcct cccttttatc cgcgatcgcg gatatcgcag cgtttatccc
     5521 gtacagcgga taagatgtgt ttccagattg actttatcct cactaaagga taaaactgat
     5581 aatatcccct taagcggata aatttgctgt ggacgtatga catgatgagc tttcagaaga
     5641 tctatagccc aacgcaatta gcgaatgcaa tgaaactggt tcgccagcaa aatggctgga
     5701 cgcagagcga gctggcgaaa aaaatcggca ttaagcaggc gacgatttcc aatttcgaaa
     5761 acaaccctga caatacctcg ctcacgacat tttttaagat tttacagtcg cttgaactct
     5821 caatgacgct atgcgacacg aaaaatgcct cgccagaatc aacagaacag caagatctgg
     5881 agtggtaatg cccaaactag tcacttggat gaacaaccag cgggtaggcg agttaacgaa
     5941 gttagccaac ggcgcgcaca cctttaagta tgcaccggag tggttagcca gccgttatgc
     6001 cagaccgttg tcactttcgc tgccattgca gagggggaat atcacctctg atgccgtatt
     6061 taacttcttc gataacttgt tacccgatag cccgattgta cgtgaccgga tcgttaaacg
     6121 ttatcatgct aaatccagac aaccgtttga tttattgtca gaaatagggc gagacagcgt
     6181 tggtgccgta acgttattac ccgaaaatga aaccataacg cgcccgataa tggcatggga
     6241 aaagcttact gaagccagac ttgaagaagt attaacggct tataaagcag atatcccgct
     6301 aggcatgatt agagaagaaa atgactttcg catctcggtt gctggcgcac aggagaagac
     6361 agcactgctc agaataggca atgactggtg cattccgaaa ggaataacgc cgacgacgca
     6421 catcattaaa ttaccgattg gggaaatcag gcagcccaat gcgacgctcg atctcagcca
     6481 aagcgttgat aatgagtatt actgtctgct gctggcgaaa gaacttgggt tgaatgttcc
     6541 ggacgcagaa atcattaaag cgggaaatgt gcgcgcgtta gcggtcgaac gttttgacag
     6601 gcgttggaat gctgagcgaa cggttttact tcgcttgcca caggaggata tgtgtcagac
     6661 attcggttta ccttcatcgg tgaaatatga atcagatgga ggcccaggca tcgcgcggat
     6721 catggctttt ttgatggggt ccagcgaggc gctgaaagat cgctatgatt ttatgaaatt
     6781 ccaggtcttt cagtggttga ttggcgcaac ggatggccat gcaaaaaact tctccgtatt
     6841 tattcaggct ggcggcagtt atcgactcac gccattttac gacatcattt cagcatttcc
     6901 ggtattgggt ggcgcgggaa tacacatcag cgatctcaaa ctggcaatgg ggcttaacgc
     6961 atccaaaggc aaaaaaacgg caatcgataa aatttatccg cgacattttt tggcgacagc
     7021 aaaggtgctg agattcccgg aagtgcagat gcatgaaatc ctgagtgact ttgccagaat
     7081 gattccggca gcactggata acgtgaagac ttcattaccg acagattttc cagagaacgt
     7141 ggtgacggca gttgaaacca atgtgttgag gttgcacggt cggttaagcc gagaatacgg
     7201 tattaagtaa gagtaagata tggggtttgg tcgttgttaa tgagcaggac aatcatgacc
     7261 gccaacgact agattcttcc ttggtttatg ttggtgcgca taatgtatat tatgttaaat
     7321 cgtgtctgag tgatacgaag tgattttccg gtagtcgtta ttcgttcagg gttccagaac
     7381 ttgagtctct cctctttcat aattggcctg tgcatgccgg atataggctg gctggtatgc
     7441 agatttgccg tttgccgcat cttcccagcg ttgaagactc aagctggtaa accgcgtgca
     7501 aatgagcacg ccctctttcc atcaacactt caggcagctt gcgggaatga gtccactccg
     7561 gtatcagaaa tggctacgtc tcaatgaagt caggcgaacg atgctgaatg aacattacga
     7621 tgtcaccaca gcagcctatg ctgtcggtta cgaaagctta tcccatttca gccgggaata
     7681 gcttcaatat caagctgaat gattgtgata ccaatgtttc cagtaatgca gctgttgcat
     7741 tcctgggtac tactgtcacc agtaatgacg atacgttagc gctgcagagt tcagcggcag
     7801 gctctgccca aaatgtcggt atccaaattt tggaccgtac gggtgaggta ttaatacttg
     7861 atggtgccac ttttagcgct aaaactgact tgattgatgg cacgaatata ctgccgttcc
     7921 aggctcgtta tattgcactc gggcagtccg tagctggtac tgcaaacgca gatgcgacct
     7981 tcaaagttca atatctataa ttcaatctta aggtggctat acgtcatctg aaattgaaac
     8041 agggtgtgtg tggcatggct acgcacaccc gagcaattca ccaatgtgaa tctcattatt
     8101 aatgaggagt ggtttgtatt tggagcgcta tgaaaaatca cataatatct aaactatcaa
     8161 taaacaacag tagttaaact tactgttatt taaaatattt actttgtagc aaggttttcc
     8221 tattaaaaga gaaccaacat ttattacaaa cgcattaagt ctggaaaccc ggcgactcgc
     8281 caatgtatta gcaacattta ccgattgtct gatgtaacgg ttttcccgct tatcctctta
     8341 acacatggag aatgcatcag atgcaaacta cgcgtacccc ctactctatt tcctttatgg
     8401 cgacagtgtt acttttattg ctctttgctt gccatagcac cgttgccaac gcagctgtcg
     8461 cactgggtgc gactcgggta atttatccgg caaaccagaa acaggtttta ttgcctgtca
     8521 cgaataatga tcctgccagt gtctatctca ttcagtcatg gatagaaaat gcaggtgacc
     8581 aaaaagacac ccagtttgtc attaccccac cgcttttttc catgcaaggt aaaaaagaga
     8641 atacgctgcg gataatcaac gccactaatc atcagttacc aggcgaccgt gaaagcctgt
     8701 tctgggttaa tgttaaagct attcctgcta tggaaaaaga ccagaagaat gagaatacat
     8761 tacagctggc aattattagc cgcataaaaa tgttttatcg ccccacgaac ctggcaatgg
     8821 cacctgaaga agcgcctgca atgcttcggt ttcgtcgcag cggtagcaaa ctcactctta
     8881 ttaatccaac gccctacttc attaccgtaa caaatatgaa agcaggaaat agcaatctcc
     8941 caaacaccat ggtgccgcca aaagatgaag tatcggtgga tatcccgcat gctgccaccg
     9001 gggatatcag ttttcagacg atcaatgatt acggtgcatt aactccgcgt attaaggcaa
     9061 ccatgcagta agccagtgac ctaattaata ataaactcaa tcggaattaa taatgacagc
     9121 ctttcgtgcc gcatttaagg catatcgtat gcatcaggtg ttactactgc ctcgctttgc
     9181 acggcttacc attgccctta gcttagcaac tgcggtattt cccgttgacg cagagtatta
     9241 ttttaatcca cggtttttaa gcaatgatct tgctgaatct gtcgatttat ccgcctttac
     9301 taagggacgc gaggcaccac ctggtacata tcgggtcgac atttatttga acgacgaatt
     9361 tatgactagt cgggatatta cttttattgc agatgataat aacgcagatc tcatcccatg
     9421 cctgagtaca gatcttcttg tcagtcttgg cataaaaaaa tctgcgttat tggacaataa
     9481 ggaacattcg gcagaaaaac atgtgccgga caacagcgaa tgcacaccac ttcaggatcg
     9541 tttggctgac gcatcaactg agtttgatgt tggtcagcaa catttgtcac tctccgttcc
     9601 gcaaatttat gttggcagaa tggctcgcgg ctatgtttct cctgatctgt gggaagaagg
     9661 aataaatgca gggcttctaa actatagttt taatggtaat tctattaata atcgtagtaa
     9721 ccataatgca ggaaaatcca actatgcata tttgaattta cagagtggca tcaacattgg
     9781 tagttggcga ctgcgcgata actcaacgtg gagttataac agtgggagta gcaattcatc
     9841 tgacagcaat aaatggcagc atatcaatac gtcggctgaa cgtgacatta ttcccttacg
     9901 ctcacggtta acggtaggtg atagttatac caatggcgat atttttgata gtgtgaactt
     9961 tcgtggcctc aaaataaatt caacagaagc gatgttgccc gatagccaac atggtttcgc
    10021 tccggtgatt catggtattg cccgtggcac cgcacaagtg agtgtaaaac agaacggata
    10081 cgatatttat cagactactg ttccacccgg cccttttact attgatgata tcaactctgc
    10141 ggccaatggt ggtgatctgc aagtaaccat aaaagaggca gacggcagta ttcagacatt
    10201 atatgttcct tattcgtctg ttccggttct ccaacgtgct ggatatacgc gttatgcgct
    10261 tgccatgggg gaatatcgta gtggaaataa cctgcaaagc tcccccaggt tcatacaagg
    10321 tagcttgatg catggactgg aaggaaactg gacaccttat ggcggaatgc aaattgcaga
    10381 agattatcag gccttcaacc ttggtattgg taaagattta agactttttg gtgccttttc
    10441 tttcgatatc acgcaggcca atacgacact tgcagatggc acccgtcaca gcgggcaatc
    10501 ggttaaatcc gtctacagca aatccttcta ccagacagga accaatatcc aggtcgcagg
    10561 atatcgctat tctacgcaag gtttttataa cttatccgac agtgcctaca gtcgaatgag
    10621 tggttacacc gtcaagcctc ctaccggaga cagcaatgag cagacacaat ttattgatta
    10681 ttttaatctg ttctacagta agcgtggtca ggaacaaata agcatctctc agcagcttgg
    10741 aaattacggt acgacatttt tcagtgccag tcgccaaagt tactggaaca cgtcacgcag
    10801 cgaccagcaa atatcatttg gattaaatgt gccgtttggt gatattacga cttcactgaa
    10861 ttacagctat tccaataata tatggcaaaa cgatcgggat catttactcg cttttacgct
    10921 taatgttccc ttcagtcatt ggatgcgtac agacagtcag tcggcatttc gtaattcaaa
    10981 cgccagttac agtatgtcaa acgatttgaa aggcggcatg accaatctat cgggggttta
    11041 tggcactctg ctgccggata ataacctgaa ttatagcgtt caggtcggta acacccacgg
    11101 aggtaataca tcgtctggca ccagtggtta cagttctctt aattatcgtg gagcttacgg
    11161 taatacgaat gtcggttaca gtcggagtgg tgacagcagc cagatctatt acggaatgag
    11221 tggtgggatt attgctcatg ctgatggcat cacctttgga cagccgctgg gcgacacaat
    11281 ggttctggtt aaggctcctg gcgctgataa tgtcaaaata gagaaccaga ccggaattca
    11341 taccgactgg cgtggctatg caatattacc atttgcgaca gaatatagag aaaatcgtgt
    11401 cgctcttaac gcgaattccc ttgcagataa tgttgaactg gatgaaaccg tagtcactgt
    11461 catcccaact cacggtgcta ttgccagagc aacatttaat gcacaaatcg gcgggaaagt
    11521 attaatgacg ttgaagtacg gtaataaaag cgttccattc ggtgcaattg tcactcacgg
    11581 agagaataaa aatggcagca ttgtcgcgga aaatggtcag gtttatctga ctggacttcc
    11641 acagtcaggg aaattacagg tttcatgggg caatgataaa aactcaaact gtattgtcga
    11701 ttacaagctt cctgaagtct ctcctggaac cttgctgaac cagcagacag caatctgtcg
    11761 ctaaggaaca atagattatg aaatacaata acgttatttt cctcggttta tgtctggggt
    11821 taaccaccta ttctgcttta tccgcagata gcgttattaa aattagcggg cgcgtcctcg
    11881 attatggctg cacagtctca tcggattcgc ttaattttac cgtagatctc caaaaaaaca
    11941 gtgccagaca atttccaacg accggtagca caagtccagc cgtccctttt cagattacgt
    12001 taagtgaatg cagcaaaggg acaacggggg ttcgggttgc atttaacggt attgaggatg
    12061 cagaaaataa tactctgttg aaactggatg agggaagcaa tacggcctcc ggtttaggta
    12121 tagaaatact ggacggaaat atgcgtccgg tgaaactgaa tgaccttcat gccgggatgc
    12181 agtggatccc actggtacca gaacagaaca atattttgcc ttactccgct cgtctgaagt
    12241 caactcagaa gtccgtcaat ccgggactgg tgagggcttc ggcaaccttt acccttgaat
    12301 ttcaataatc agagtcagat atgaaaagac ttcacaagag gttcctgtta gctacgtttt
    12361 gcgcgttatt cacagcaact ctccaggccg ccgatgtcac tatcactgtt aatggtcggg
    12421 tagtcgctaa accctgcact attcaaacca aagaagctaa cgttaatctc ggggatcttt
    12481 atacgcgcaa tctgcaacaa cctggttctg catctggctg gcacaatatt actttgtcat
    12541 taaccgattg tccggctgaa acaagtgcag tgacggcaat cgtgacaggt tcaactgaca
    12601 atacgggtta ttacaaaaat gaaggtactg ccgaaaatat tcagatagag cttagggatg
    12661 accaggatgc gacgttaaaa aatggcgata gcaaaacggt tattgttgat gagatcactc
    12721 gtaatgcaca gtttccactt aaggcaagag ctatcacggt gaatggaaac gcaagccagg
    12781 gaacgatcga ggcgctaatc aatgtgatct acacctggca ataacgtcga ttcgattttc
    12841 ttagccttct ttttaaatca attttttaac cttaggatat atcatgggta aaacaatcag
    12901 tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt ttgccttcag
    12961 ttgtaatgtc gatggcgggt caagtattgg agctggtaca acctctgttt atgttaatct
    13021 cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc atataagttg
    13081 ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac aaggctcagc
    13141 tttcgcaggc tctctacagt catataaagg atcgctgtac tggaataatg tgacctaccc
    13201 cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac cgatgccatt
    13261 accgttaaaa ctctatatta ccccagtcgg ggcggccggt ggagttgtca taaaagccgg
    13321 ggaagttatt gcccgtattc atatgtataa aattgccacg ttaggaagcg gaaatccgcg
    13381 taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag gcggctgcac
    13441 tgttgatagt cgtaatgtca ccgtgaatct cccggacttt cctggaagcg cagaaatccc
    13501 ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg gtgccaccac
    13561 tgacagtgcc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag catcgggagt
    13621 gggtgtctct ctgatgcgta atggaaaaat actggctacg ggagaaaata tctccctggg
    13681 gacagtaaat aaaagcaaag tacctctggg tttaagcgca acctatggac aaacaggaaa
    13741 caaggtttca gccggaaccg tccagtcagt cattggtgtc acttttatct atgaataatt
    13801 gtgattttga cattggtcta tgcgctccat tcacgaaaat tgcgcggata attattttgt
    13861 gaaggctatt atcctacacc tgtaagaa
//
LOCUS       DAZKFU020000120        13860 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZKFU020000120 DAZKFU020000000
VERSION     DAZKFU020000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.121"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..1151
                     /gene="citC"
                     /locus_tag="ACHOQQ_RS14220"
                     /old_locus_tag="pgaptmp_002844"
     CDS             93..1151
                     /gene="citC"
                     /locus_tag="ACHOQQ_RS14220"
                     /old_locus_tag="pgaptmp_002844"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGU4471065.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTKG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1166..1462
                     /gene="citD"
                     /locus_tag="ACHOQQ_RS14225"
                     /old_locus_tag="pgaptmp_002845"
     CDS             1166..1462
                     /gene="citD"
                     /locus_tag="ACHOQQ_RS14225"
                     /old_locus_tag="pgaptmp_002845"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGU4471066.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1459..2367
                     /gene="citE"
                     /locus_tag="ACHOQQ_RS14230"
                     /old_locus_tag="pgaptmp_002846"
     CDS             1459..2367
                     /gene="citE"
                     /locus_tag="ACHOQQ_RS14230"
                     /old_locus_tag="pgaptmp_002846"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGU4471067.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2378..3910
                     /gene="citF"
                     /locus_tag="ACHOQQ_RS14235"
                     /old_locus_tag="pgaptmp_002847"
     CDS             2378..3910
                     /gene="citF"
                     /locus_tag="ACHOQQ_RS14235"
                     /old_locus_tag="pgaptmp_002847"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGU4471068.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3914..4465
                     /gene="citX"
                     /locus_tag="ACHOQQ_RS14240"
                     /old_locus_tag="pgaptmp_002848"
     CDS             3914..4465
                     /gene="citX"
                     /locus_tag="ACHOQQ_RS14240"
                     /old_locus_tag="pgaptmp_002848"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGU4471069.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALCALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCAQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4440..5318
                     /gene="citG"
                     /locus_tag="ACHOQQ_RS14245"
                     /old_locus_tag="pgaptmp_002849"
     CDS             4440..5318
                     /gene="citG"
                     /locus_tag="ACHOQQ_RS14245"
                     /old_locus_tag="pgaptmp_002849"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGU4471070.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5369..6832
                     /gene="citT"
                     /locus_tag="ACHOQQ_RS14250"
                     /old_locus_tag="pgaptmp_002850"
     CDS             5369..6832
                     /gene="citT"
                     /locus_tag="ACHOQQ_RS14250"
                     /old_locus_tag="pgaptmp_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGU4471071.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6946..7752
                     /gene="rna"
                     /locus_tag="ACHOQQ_RS14255"
                     /old_locus_tag="pgaptmp_002851"
     CDS             6946..7752
                     /gene="rna"
                     /locus_tag="ACHOQQ_RS14255"
                     /old_locus_tag="pgaptmp_002851"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGU4471072.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEMQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            7982..8392
                     /gene="rnk"
                     /locus_tag="ACHOQQ_RS14260"
                     /old_locus_tag="pgaptmp_002852"
     CDS             7982..8392
                     /gene="rnk"
                     /locus_tag="ACHOQQ_RS14260"
                     /old_locus_tag="pgaptmp_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGU4471073.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8530..8619
                     /gene="yldA"
                     /locus_tag="ACHOQQ_RS14265"
                     /old_locus_tag="pgaptmp_002853"
     CDS             8530..8619
                     /gene="yldA"
                     /locus_tag="ACHOQQ_RS14265"
                     /old_locus_tag="pgaptmp_002853"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGU4471074.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8623..9861)
                     /gene="ybdR"
                     /locus_tag="ACHOQQ_RS14270"
                     /old_locus_tag="pgaptmp_002854"
     CDS             complement(8623..9861)
                     /gene="ybdR"
                     /locus_tag="ACHOQQ_RS14270"
                     /old_locus_tag="pgaptmp_002854"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HGU4471075.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10081..10509
                     /gene="uspG"
                     /locus_tag="ACHOQQ_RS14275"
                     /old_locus_tag="pgaptmp_002855"
     CDS             10081..10509
                     /gene="uspG"
                     /locus_tag="ACHOQQ_RS14275"
                     /old_locus_tag="pgaptmp_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGU4471076.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10630..12195)
                     /gene="ahpF"
                     /locus_tag="ACHOQQ_RS14280"
                     /old_locus_tag="pgaptmp_002856"
     CDS             complement(10630..12195)
                     /gene="ahpF"
                     /locus_tag="ACHOQQ_RS14280"
                     /old_locus_tag="pgaptmp_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGU4471077.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12324..12887)
                     /gene="ahpC"
                     /locus_tag="ACHOQQ_RS14285"
                     /old_locus_tag="pgaptmp_002857"
     CDS             complement(12324..12887)
                     /gene="ahpC"
                     /locus_tag="ACHOQQ_RS14285"
                     /old_locus_tag="pgaptmp_002857"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGU4471078.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13259..>13860
                     /gene="dsbG"
                     /locus_tag="ACHOQQ_RS14290"
                     /old_locus_tag="pgaptmp_002858"
     CDS             13259..>13860
                     /gene="dsbG"
                     /locus_tag="ACHOQQ_RS14290"
                     /old_locus_tag="pgaptmp_002858"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGU4471079.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLKVPANVSTEQMKV"
BASE COUNT         3252 a         3697 c         3507 g         3404 t
ORIGIN      
        1 cccaagtgaa aataaaaggt tatcagtttg caacattgaa caacattcgt tgcaaatcga
       61 taacaacatg caccttcagg atactattta ttatgttcgg caatgatatt ttcacccgcg
      121 taaaacgttc agaaaataaa aaaatggcgg aaatcgccca attcctgcat gaaaatgatt
      181 tgagcgttga caccacagtc gaagtattta ttaccgtaac ccgcgatgaa aagcttatcg
      241 cgtgcggtgg aattgccgga aatattatta aatgcgttgc tatcagtgaa tccgttcgcg
      301 gtgaaggact ggcgctgaca ttagccactg aactgataaa cctcgcctat gagcggcaca
      361 gcacgcatct gtttatttat accaaaaccg aatacgaggc gctgttccgc cagtgcggtt
      421 tttccacgtt gaccagcgta ccaggcgtga tggtgctgat ggaaaacagc gccacgcgat
      481 tgaaacgcta tgccgaatcg ctgaaaaaat ttcgtcatcc cgggaacaag attggctgca
      541 ttgtgatgaa cgccaatccc tttacgaagg gtcaccgtta tctgattcaa caggctgcgg
      601 cacagtgcga ctggttgcat ctgtttttag tcaaagaaga ttcttcacgc ttcccctatg
      661 aagaccgact ggatctggtg ttaaaaggca ccgccgatat tccacgcctg actgtgcatc
      721 gcggctccga atacatcatc tcccgcgcta cgttcccttg ctacttcatt aaagaacaga
      781 gcgtcattaa ccattgttac accgaaattg atttgaagat tttccgtcag tacctcgctc
      841 ccgcgctggg tgtaactcac cgctttgtcg gtactgaacc cttttgtcgc gttaccgccc
      901 agtacaacca ggatatgcgc tactggctgg aaacgccgac tatctccgca ccgcccatcg
      961 aactggttga aattgagcgg ctgcgttacc aggagatgcc gatatccgct tcccgggtac
     1021 gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc gctggtccct gcagtcacgc
     1081 tgcattattt gcagaacctg cttgagcact cccgccagga cgcggcagct cgtcaaaaga
     1141 cccccgcatg agaaacaggt gaaaaatgaa aataaaccag cccgccgttg caggcaccct
     1201 tgagtctggg gatgtgatga tacgcatcgc cccactcgat acgcaggata tcgacctgca
     1261 aatcaatagc agcgttgaga aacagtttgg cgatgcaatt cgcaccacca ttctggacgt
     1321 tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc gatgacaaag gcgcactgga
     1381 ctgcatttta cgtgcacgac tggaagccct gctggcacgc gccagcggta tcccggctct
     1441 gccatgggag gattgccaat gatttccgct tcgctgcaac aacgtaaaac tcgcacccgc
     1501 cgcagcatgt tgtttgtgcc tggtgccaat gccgcgatgg tcagcaactc cttcatctac
     1561 ccggctgatg ccctgatgtt tgacctcgaa gactccgtag cattgcgtga aaaagacacc
     1621 gcccgccgca tggtttacca cgcgctgcaa catccgctgt atcgcgatat tgaaaccatt
     1681 gtgcgtgtca acgcgctgga ttccgaatgg ggtgtcaacg acctggaagc cgtcgttcgc
     1741 ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg ctcaggatgt tctggatatc
     1801 gaaaaagaga tcctgcgtat cgaaaaagcc tgtggtcgtg aacccggcag caccggcctg
     1861 ctggcggcga ttgaatctcc gctggggatt acccgcgcag tggaaatcgc tcacgcttcc
     1921 gagcgtttga tcggtatcgc cctcggtgca gaagactatg tgcgcaacct gcgtacagaa
     1981 cgctccccgg aaggaactga actgctgttc gcacgctgtt ccattttgca ggccgcgcgc
     2041 tctgcgggta ttcaggcgtt cgataccgtc tattccgacg ctaacaacga agccggattc
     2101 ctgcaagaag ccgcccacat caagcagctg ggctttgacg gcaaatcgct gatcaacccg
     2161 cgtcagattg atctgctgca caacctctac gcaccgaccc agaaagaagt ggatcacgcc
     2221 cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt ggtttccctg
     2281 aacggcaaga tggtggacgg tccggttatc gatcgcgccc gtctggtgct ctcccgtgca
     2341 gaactttccg gcatccgcga agaataaggc aatcaaaatg acgcagaaaa ttgaacaatc
     2401 tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct gaatgcgatc ttgccgcttt
     2461 ccagaactcg ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca aactgtgcgc
     2521 caacctggaa gaagcgattc gtcgatcagg tttacaggac ggcatgacgg tttccttcca
     2581 tcacgctttc cgtggcggtg acctgaccgt caatatggtg atggacgtca tcgcgaagat
     2641 gggctttaaa aacctgaccc tggcgtccag ctccctgagt gattgccatg cgccgctggt
     2701 agaacacatt cgccagggcg tggttacccg catttatacc tccggcctgc gcggtccact
     2761 ggcagaagaa atttcccgtg gtctgcttgc ggaaccggtg caaatccact ctcacggcgg
     2821 tcgcgtgcat ctggtacaga gtggcgaact gaatatcgac gtggctttcc tcggcgtccc
     2881 gtcctgcgat gaattcggta atgccaacgg ctacaccggt aaagcctgct gcggctccct
     2941 cggctatgca atggtcgatg ccgacaacgc aaaacaggtc gtgatgctca ccgaagaact
     3001 gctgccttat ccgcataatc cggcaagcat tgaacaagat caggttgatc tgatcgtcaa
     3061 agttgatcgc gttggcgatg ctgcaaaaat cggcgctggt gcgacccgta tgaccactaa
     3121 cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg attgtcaact ctggctactt
     3181 caaagaaggt ttctccatgc aaaccggcac cggcggcgca tcgctggcgg taacccgttt
     3241 cctggaagac aaaatgcgta gccgcgatat tcgcgccgac ttcgcccttg gcggtattac
     3301 cgcgacgatg gttgacctgc acgaaaaagg tctgatccgc aaactgctgg atgtgcagag
     3361 ctttgacagc catgctgcgc agtcgctggc ccgtaacccc aatcacatcg aaatcagcgc
     3421 caaccagtac gctaactggg gttcgaaagg cgcatcggtt gatcgtctcg acgtggtggt
     3481 actgagcgcg ctggaaattg acacccagtt caacgttaac gtgctgaccg gctctgacgg
     3541 cgtactgcgt ggtgcttccg gtggtcactg cgataccgca attgcctctg cgctttccat
     3601 catcgtcgcg ccgctggtac gcggtcgtat tccgactctg gtggataacg tgctgacctg
     3661 catcaccccg ggctccagtg tcgatattct ggtcacagac cacggtatcg cagttaaccc
     3721 ggcacgtccg gaactggcag aacgtctgca ggaagcgggc attaaagtgg tttccattga
     3781 gtggctgcgc gaacgcgcgc gtctgctgac cggtgaacca cagccgattg aattcaccga
     3841 ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg atcgatgttg tgcatcaggt
     3901 gaaggaataa gccatgcacc tgcttcctga actcgccagc caccatgcgg tgtcaattcc
     3961 cgagctgctc gtcagccggg atgaaaggca agcacggcaa cacgcctggc tcaagcgcca
     4021 tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg ccgattaaag acagcgaggt
     4081 cacacgccga atttttaatc atggcgtgac cgccttgtgt gccttagccg caaaacaggg
     4141 ctggcaaatt caggagcagg ctgcactggt ttccgccagc gggccggagg gcatgttgag
     4201 cattgccgcc ccggctcgcg acctcaagct cgccaccatt gagcttgaac atagtcatcc
     4261 tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa ggcgaaattc tctcccgccg
     4321 cgactattca ctgccgcctc gccgctgcct gttgtgcgcg caaagcgcag ccgtctgcgc
     4381 gcgtggaaaa acccatcaac tgaccgattt actcaaccgc atggaggcac tgctgaacga
     4441 tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc gacgtcatta atcgatgagt
     4501 acgccctgct gggctggcgc gccatgctga ctgaagtcaa tctgtcaccg aaaccaggcc
     4561 tcgtggatcg cattaactgc ggtgcgcata aagatatggc gctggaagat ttccaccgca
     4621 gcgcgctggc gattcagggc tggttacccc gtttcattga atttggtgcc tgtagtgcag
     4681 aaatggcacc ggaagcggta ctccacggat tacgcccaat tggtatggct tgcgaaggtg
     4741 atatgttccg cgccactgcg ggcgtaaaca cgcataaagg cagcattttt tctttagggc
     4801 tgctatgtgc ggcaattgga cgtttgcttc agctcaacca gccggttacg ccaacaaccg
     4861 tttgttctac ggcggcaagt ttctgccgtg gcctgaccga tcgcgaactg cgtaccaata
     4921 attcacaact gacggcaggt caacggttgt accagcagct tggccttacc ggcgcacgcg
     4981 gtgaagccga agcgggttat ccactggtga tcaaccacgc cttgccgcat tacctcactc
     5041 tgctggatca ggggttagat cctgaactgg cattgctcga taccttgctc ctactgatgg
     5101 cgatcaacgg cgataccaac gttgcgtcgc gcggtggcga ggggggcctg cgctggctac
     5161 agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg aacccccgcc gatctcgatt
     5221 atctccggca gttcgacagg gagtgtatcg aacgaaatct cagtccaggc ggcagtgccg
     5281 acctgctgat ccttacctgg tttttagcac agatttaatt atttaagcac ttgataaatt
     5341 ttgaaatatt aattttcgga gaacccgtat gtctttagca aaagataata tatggaaact
     5401 attggcccca ctggtggtga tgggcgtcat gtttcttatc cctgtccccg acggtatgcc
     5461 gccgcaggca tggcattact tcgctgtgtt tgtggcaatg attgtcggca tgatcctcga
     5521 gccaattccg gcaacagcga tcagttttat tgcggttact atttgcgtta ttggcagtaa
     5581 ttacctgctc tttgatgcca aagaattagc tgacccagcg tttaatgcgc aaaaacaggc
     5641 gctgaaatgg ggtctggctg gtttttccag caccacggta tggctggtat ttggcgcatt
     5701 tatttttgca ttagggtatg aagtttccgg gttaggtcgt cgcattgccc ttttcctggt
     5761 gaaattcatg ggcaaacgca cgctgacgtt gggttatgcg attgtcatta tcgacattct
     5821 gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg ggtacggttt ttccggtcat
     5881 taaaaacctg ccgccgctgt ttaaatcatt cccgaacgat ccgtccgcgc gtcgtattgg
     5941 cggctatttg atgtggatga tggtcattag taccagtctg agttcgtcca tgtttgtcac
     6001 cggtgcggca ccaaacgtgc tgggtctgga gttcgtcagc aaaattgccg gtatccagat
     6061 tagctggttg cagtggttcc tctgcttcct gccggttggg gttatcttgc ttatcattgc
     6121 gccgtggctt tcctacgtgc tgtacaaacc ggaaatcaca cacagtgaag aagtggcaac
     6181 ctgggcgggt gatgaactga aaaccatggg tgcgctgaca cgcagagagt ggacactgat
     6241 tggccttgta ttgctcagct taggtttgtg ggtatttggc agtgaagtca ttaatgctac
     6301 tgcggtaggt ctgctggcag tttcgctaat gctggccctg cacgttgtgc cgtggaaaga
     6361 cattacccgc tataacagcg catggaacac gctggtcaac ctggcaactc tggttgtgat
     6421 ggctaacggc ctgactcgtt ctggctttat tgactggttc gccggtacca tgagtacgca
     6481 cctggaagga ttctcaccaa acgcaacggt gattgtactg gttctggtgt tctactttgc
     6541 acactacctg tttgccagcc tgtctgcgca caccgcaacc atgctgccgg ttattctggc
     6601 cgtcggtaaa ggtattccgg gcgtaccaat ggaacaactg tgtatcctgc tggtgctgtc
     6661 tattggtatc atgggctgtc tgacgccgta tgcaaccggt cctggggtga ttatttacgg
     6721 ctgtggctat gtgaaatcaa aagattactg gcgtcttggc gcaatcttcg gggtgattta
     6781 catctctatg ttgctgttgg ttggctggcc gattctcgcc atgtggaact aatacgttcg
     6841 ccaaacgctg aataaatcat cacgcccgcc aggtaagccc acctggcggg cttttttatg
     6901 atttaataga tagtattcag tgtatgtatg agttccacac ccattatgaa agcattctgg
     6961 cgtaacgccg cgttgctcgc ggtttctctg cttcccttct cttctgccaa cgccgtagcg
     7021 ttgcaggcaa aacagtatgg cgattttgat cgctatgtcc tggccctctc ctggcaaacc
     7081 ggattttgcc agagtcaaca cgatcgaaat cgtaacgaac gagatgaatg tcgcctgcaa
     7141 accgaaacga ccaacaaagc tgattttctg accgtacatg gtctgtggcc aggattgcct
     7201 aaatcggtcg ctgcccgtgg tgttgatgaa cgccgctgga tgcgcttcgg ttgcgctact
     7261 cgcccaatcc cgaatctacc agaagcgcgc gccagccgaa tgtgttcatc gccggaaacc
     7321 gggttatcac tggaaacggc cgctaaacta agtgaagtca tgccaggagc tggcggacgt
     7381 tcctgcctgg aacgctacga atatgccaaa cacggtgcct gctttggttt tgatccggac
     7441 gcatacttcg gtacgatggt acgcctgaat caagaaatta aagagagcga agccggaaaa
     7501 ttccttgcgg ataattacgg taaaacagtg agccgccgtg actttgacgc cgcctttgcc
     7561 aaaagctggg gaaaagagaa cgtgaaagca gttaagctaa cgtgccaggg taaccctgcg
     7621 tatttgactg aaatgcagat ctcgatcaaa gctgacgcca tcaacgctcc gctttctgcc
     7681 aactcattct tgccacaacc acacccaggc aactgtggca aaacctttgt gattgataaa
     7741 gcgggttatt aatcctctga tttacaaaac acgctaattc gtcttccata aaccaaggcg
     7801 attaagattt atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact
     7861 tcaaagagtg agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg
     7921 agggttttct tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa
     7981 aatgtccaga ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct
     8041 ggagcaaccc gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg
     8101 cgcccaaatg tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt
     8161 taaattccgc aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa
     8221 aatgaccgat agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg
     8281 gctgcgcgtt ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga
     8341 agtgctggaa ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct
     8401 gcacaagcga taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca
     8461 gcaatttttc ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg
     8521 ggaataacca tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt
     8581 atcgtcatgg ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg
     8641 catcgcattc accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg
     8701 taccagaatc accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc
     8761 ggcctcttca aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc
     8821 aattaacggt aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt
     8881 caacccttta tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc
     8941 cggtacgcta acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc
     9001 gctgctgccc tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc
     9061 aaaaccgacg gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat
     9121 tgactgtgcc ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc
     9181 ggcgaaacgc aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa
     9241 ccgtgcacag gcgattgtca acaatcccac aggaccggca ccatagactg caacgcttga
     9301 accttgttgg atctgcgcat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag
     9361 gaaaagcgct ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt
     9421 agggacgcgg acatattccg cctgcccgcc aggaacgcca ccatacaggt gactataacc
     9481 aaacaatgcc gcgggagcag gtatctgttt tttattgagc gcagcgcctt tacccgcatt
     9541 ggtattttcg caggcggcat attgctgcat tcgacagaaa aaacagtcgc cacaagcaat
     9601 gacgaacgga attaccaccc ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc
     9661 aactacttcc cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt
     9721 gcctcgataa agatggaggt cagagccaca gatcgccgtt gccgtaatac gcagaataat
     9781 atcatctgcc tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc
     9841 gtgataagtc aatgctttca tgcaacctcc agagacagtt caaaaaaagt cagtctttaa
     9901 gggtagctat cacagcggga aaacgggcgg taccgcaggt tttgggagaa ttcctggtgt
     9961 tcagtgagaa tatattaatc acagaaaccg ttcgtaagga aaaagaacca gacaaatatc
    10021 agggataaat attataatca tggcgaatga tttgttcatg attaacaggg agaaagggtt
    10081 atgtataaga caatcattat gccagttgat gtatttgaaa tggaattgag cgacaaagct
    10141 gttcgccacg ctgaattcct cgcccaggat gacggagtta ttcatctact tcacgtacta
    10201 cccgggtcag ccagcctgag cctgcaccgt tttgccgctg atgtgcgtcg ttttgaagag
    10261 catctgcaac atgaagcaga agaacgtctg caaacgatgg tcagccactt caccatcgat
    10321 ccttcccgca ttaaacaaca tgtccgtttt ggtagcgtgc gggatgaagt caatgagttg
    10381 gcagaagaac tgggggctga tgttgtagtt atcggttctc gcaacccatc gatttcgacc
    10441 catctgttag gttctaacgc ctcgagcgtg atccgccacg ccaatctgcc tgtactggtc
    10501 gttcgctaac agataaaaat atttatgccg ccctgcctgg aacatcccgg cagggctttt
    10561 taatgccata aaaaagccgc caggtttgac cccggcggct aagcaattgc aggtgaatct
    10621 tacttcttct tatgcagttt tggtgcgaat caggtagtca aaagcactca gagaggcttt
    10681 ggcaccttcg ccagtggcga tgatgatctg cttgtacgga accgtcgtac agtcacccgc
    10741 tgcgaacacg cctttgacgt tggtttcgca tttcgcatca atgataatct cgcccatgcg
    10801 gttacgttcg actgcgcctt ccagccagtt ggtgttcggc agcagaccaa tctggacaaa
    10861 aataccggcc agttcgatgt tgtgaatatc gccgctgaca cgatctcgat attccagacc
    10921 aacgactttg ctgccgtcgc ctttcacttc cgtggtttgc gcattcagaa taatgtcgac
    10981 gtttttcagg ctgcgcagtt tgtcctgcag aacctggtcg gctttcattt ctggcgcaaa
    11041 ttccagcagc gttacgtgct caacgatacc cgccaggtcg attgccgctt ccacgccgga
    11101 gttaccgccg ccgataaccg ctacgcgttt gcctttaaac agcgggccgt cgcagtgcgg
    11161 gcagtaggtc acgcctttag tgcgatactg atcttcgccc ggaacgttca tgttgcgcca
    11221 ttttgcaccg gtcgccacga taatgctgcg tgctttcagt accgcgccag aagctgtttc
    11281 aatctggtgt agaccacctt caactgctgc cgggatcagt ttgctggcgc tctggctgtc
    11341 gatcacatca acgtcgtatt catcaacgtg aactttcagc gcacctgcca gtttctggcc
    11401 ttcggtcttc ggtacagaaa tgtagttttc gatatcaacg gtatcgagga tctgaccacc
    11461 aaaacgttcg cccatcagac cggtacggat gcctttacgt gcggagtaaa ttgctgccgc
    11521 tgcacccgcc gggccggaac caacgattaa tacgtcataa gcatcacgct tgttcagctc
    11581 ttctgccgca cgtttttccg cgccagtatc aattttggca acgatttcag tcaacgtcat
    11641 gcggccctga ccaaactctt tcccgtttac gaacactgcc ggaacgccca tcacgttgcg
    11701 atcggtgatt tcgttctgga aggtgccgcc gtcaattgca gtgtgcttga tgcgcgggtt
    11761 caatacgctc atcaggttca gcgcctgcac cacgtccggg cagttatggc aagagagcga
    11821 gtaataggtt tcgaattcaa aatcaccgtc aatatggcga atctgctcca gcagagactg
    11881 cgcttctttc gacggatgac caccggtcca cagtaacgcc agtaccagcg aggtgaactc
    11941 gtggcccagc ggggagcctg caaaacgtgg tccctggtta gaacctgggt tggtgatcag
    12001 gaaagacggc ttacgcaccg gcaagctgtt atcttcttta aaggtgactt tgtctgacag
    12061 ttctgcgatt tcagccaaca gttccttgat ttctgccgat ttagcgctgt catccagcgt
    12121 ggcaattaac tcaacaggct tggtcaattt ctcaaggtaa gccttgagtt gagttttcat
    12181 atttgtgtcg agcatgttta tctcctgggc tttaaacatc atcatgcaag cggcctctgc
    12241 ggctacctgg atgcagcttg catcatggtg cggggaagaa aatcgggtgc cgcagcaccc
    12301 gattaattac gcgattgcgg aacttagatt ttaccaacca ggtccagaga cggagccaga
    12361 gttgcttcac cttctttcca tttagccggg caaacttcac ctgggtgaga agctacgtac
    12421 tgtgctgctt tgattttacg cagcaggtca gacgcgtcac ggccaatgcc ttcagcggta
    12481 acttcgattg cctggatgat accctgcggg tcaacaacga aggtcgcacg gtcagccaga
    12541 ccttcatctt cacgcatgtt gtcgaagtta cgggtcaggg cgccagtcgg gtcgccgatc
    12601 atcgcatatt tgattttagc gatggtttca gagctgctgt gccatgcttt gtgggtgaag
    12661 tgagtatcgg tagatactgc gtatacgtct acgcccagtt tctgcagttc ttcgtagtgg
    12721 tcagcaacgt cacccagttc ggtcgggcat acgaaagtaa agtcagccgg gtagaagaag
    12781 aagacgctcc agcggccttc ggtatctttt tcggtgattt cgatgaattc gccgtttttg
    12841 aatgcctggt ttttaaaagg tttaattttg gtgttaatca aggacatcta tacttcctcc
    12901 gtgttttcga tgagatgtaa ggtaaccaat ttttaccgat tcggctaatg cgtttccatt
    12961 atcaaatcga taagttttac cttacaacct tcgtaagaca actttgtgaa ctttgcaccg
    13021 ccagcggcga taaaaagtaa aatggccccc tttccaggtg accgtgtaat ctgaactacc
    13081 ctttggtatc ttcagcgcct gtctgcaggc tgcggtgttg ctcttacaaa tgcaacaacg
    13141 acatggatta caacaccctc ataaacaaag ggcaatcacc tgatctaagc tcttacctat
    13201 gacaatgata ggttatgcct tttactcgac ttttgcactg actgaaaagg acaaattaat
    13261 gttaaaaaag atacttttac tggctctgct tcctgcaatc gccttcgcag aggaacttcc
    13321 tgctccagta aaagcgattg aaaaacaggg tattacgatc atcaaaacgt tcgatgcccc
    13381 cggaggaatg aaaggttatc tcggaaagta tcaggatatg ggcgtcacca tctacctgac
    13441 tccagatggt aagcacgcta tctctggtta catgtacaac gagaaaggtg aaaacctgag
    13501 taacacactt atcgaaaaag aaatttacgc accagccgga cgcgaaatgt ggcaacggat
    13561 ggaacaatcc cactggctcc tcgacggtaa aaaagacgcg ccggtcattg tctacgtctt
    13621 cgccgatccg ttctgcccat attgtaaaca gttctggcag caggcgcgcc cgtgggtaga
    13681 ttctggcaaa gtacagttga gaacattgtt ggttggggtt atcaaaccag aaagtccggc
    13741 gacagccgcg gcaattcttg cctccaaaga tcccgcaaaa acctggcaag aatatgaagc
    13801 ctctggtggc aagcttaagc taaaggtgcc tgcaaacgta agtacagagc aaatgaaagt
//
LOCUS       DAZKFU020000121        13737 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZKFU020000121 DAZKFU020000000
VERSION     DAZKFU020000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.122"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(436..675)
                     /locus_tag="ACHOQQ_RS14295"
                     /old_locus_tag="pgaptmp_002859"
                     /pseudo
     CDS             complement(436..675)
                     /locus_tag="ACHOQQ_RS14295"
                     /old_locus_tag="pgaptmp_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(1215..1577)
                     /locus_tag="ACHOQQ_RS14300"
                     /old_locus_tag="pgaptmp_002860"
     CDS             complement(1215..1577)
                     /locus_tag="ACHOQQ_RS14300"
                     /old_locus_tag="pgaptmp_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471080.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            complement(1568..2104)
                     /gene="yfdR"
                     /locus_tag="ACHOQQ_RS14305"
                     /old_locus_tag="pgaptmp_002861"
     CDS             complement(1568..2104)
                     /gene="yfdR"
                     /locus_tag="ACHOQQ_RS14305"
                     /old_locus_tag="pgaptmp_002861"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4471081.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YNVAQHAVLCSKLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            complement(2232..3056)
                     /gene="yfdQ"
                     /locus_tag="ACHOQQ_RS14310"
                     /old_locus_tag="pgaptmp_002862"
     CDS             complement(2232..3056)
                     /gene="yfdQ"
                     /locus_tag="ACHOQQ_RS14310"
                     /old_locus_tag="pgaptmp_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HGU4471082.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAQFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(3122..3484)
                     /gene="yfdP"
                     /locus_tag="ACHOQQ_RS14315"
                     /old_locus_tag="pgaptmp_002863"
     CDS             complement(3122..3484)
                     /gene="yfdP"
                     /locus_tag="ACHOQQ_RS14315"
                     /old_locus_tag="pgaptmp_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGU4471083.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(4153..4827)
                     /gene="ymfK"
                     /locus_tag="ACHOQQ_RS14320"
                     /old_locus_tag="pgaptmp_002864"
     CDS             complement(4153..4827)
                     /gene="ymfK"
                     /locus_tag="ACHOQQ_RS14320"
                     /old_locus_tag="pgaptmp_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4471084.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYTIKA"
     gene            4918..5118
                     /gene="ymfT"
                     /locus_tag="ACHOQQ_RS14325"
                     /old_locus_tag="pgaptmp_002865"
     CDS             4918..5118
                     /gene="ymfT"
                     /locus_tag="ACHOQQ_RS14325"
                     /old_locus_tag="pgaptmp_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HGU4471085.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            5162..5713
                     /gene="ymfL"
                     /locus_tag="ACHOQQ_RS14330"
                     /old_locus_tag="pgaptmp_002866"
     CDS             5162..5713
                     /gene="ymfL"
                     /locus_tag="ACHOQQ_RS14330"
                     /old_locus_tag="pgaptmp_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGU4471086.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            5859..6038
                     /locus_tag="ACHOQQ_RS14335"
                     /old_locus_tag="pgaptmp_002867"
     CDS             5859..6038
                     /locus_tag="ACHOQQ_RS14335"
                     /old_locus_tag="pgaptmp_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4471087.1"
                     /translation="MRELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            6001..6546
                     /locus_tag="ACHOQQ_RS14340"
                     /old_locus_tag="pgaptmp_002868"
     CDS             6001..6546
                     /locus_tag="ACHOQQ_RS14340"
                     /old_locus_tag="pgaptmp_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HGU4471088.1"
                     /translation="MILLSVKDHQHIDSAIPGRYTVQAPYKAGAGIGVLELIKAIYDA
                     PASFLSSAFAHIKVMVGWAGASKDAPVSLITGYANPVQFTTSEIGVSGGGSISPIKEA
                     AIMATVPALTRLNDEDLHKLSYVTTALRALRKVTLSDPQAHQVLVETLINLQAERIRL
                     ADKANFHIHRLLNISGGHRHA"
     gene            6539..6775
                     /locus_tag="ACHOQQ_RS14345"
                     /old_locus_tag="pgaptmp_002869"
     CDS             6539..6775
                     /locus_tag="ACHOQQ_RS14345"
                     /old_locus_tag="pgaptmp_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471089.1"
                     /translation="MLNPLILNIYRLFQRKKTSIPTVGQWYTTPAGHVLRVSLVDREC
                     QKVICEPLGRNYRVSMPLITFRSGKNMKHLGGAA"
     gene            6772..7590
                     /locus_tag="ACHOQQ_RS14350"
                     /old_locus_tag="pgaptmp_002870"
     CDS             6772..7590
                     /locus_tag="ACHOQQ_RS14350"
                     /old_locus_tag="pgaptmp_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4471090.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASVDESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKSSYDNVDYSQIPAGFRW"
     gene            7587..8081
                     /gene="yfdN"
                     /locus_tag="ACHOQQ_RS14355"
                     /old_locus_tag="pgaptmp_002871"
     CDS             7587..8081
                     /gene="yfdN"
                     /locus_tag="ACHOQQ_RS14355"
                     /old_locus_tag="pgaptmp_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4471091.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMEAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            8081..8734
                     /locus_tag="ACHOQQ_RS14360"
                     /old_locus_tag="pgaptmp_002872"
     CDS             8081..8734
                     /locus_tag="ACHOQQ_RS14360"
                     /old_locus_tag="pgaptmp_002872"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4471092.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGTAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            8731..9057
                     /locus_tag="ACHOQQ_RS14365"
                     /old_locus_tag="pgaptmp_002873"
     CDS             8731..9057
                     /locus_tag="ACHOQQ_RS14365"
                     /old_locus_tag="pgaptmp_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGU4471093.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKA"
     gene            9054..9443
                     /locus_tag="ACHOQQ_RS14370"
                     /old_locus_tag="pgaptmp_002874"
     CDS             9054..9443
                     /locus_tag="ACHOQQ_RS14370"
                     /old_locus_tag="pgaptmp_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4471094.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            9463..10272
                     /locus_tag="ACHOQQ_RS14375"
                     /old_locus_tag="pgaptmp_002875"
     CDS             9463..10272
                     /locus_tag="ACHOQQ_RS14375"
                     /old_locus_tag="pgaptmp_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4471095.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVQELTDTQNLGVGPVNTVRGGPKQGSYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVVSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLRQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            10280..11269
                     /locus_tag="ACHOQQ_RS14380"
                     /old_locus_tag="pgaptmp_002876"
     CDS             10280..11269
                     /locus_tag="ACHOQQ_RS14380"
                     /old_locus_tag="pgaptmp_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4471096.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLVLKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVTFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            11283..12035
                     /gene="ydfT"
                     /locus_tag="ACHOQQ_RS14385"
                     /old_locus_tag="pgaptmp_002877"
     CDS             11283..12035
                     /gene="ydfT"
                     /locus_tag="ACHOQQ_RS14385"
                     /old_locus_tag="pgaptmp_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4471097.1"
                     /translation="MNLEALPKYYSPKSPKLSDAAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKAGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEPCAGTGFHNVLREVVKYSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARCHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEVQLRKVTR"
     gene            complement(12307..12396)
                     /locus_tag="ACHOQQ_RS14390"
                     /old_locus_tag="pgaptmp_002878"
     CDS             complement(12307..12396)
                     /locus_tag="ACHOQQ_RS14390"
                     /old_locus_tag="pgaptmp_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471098.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLTVIALLIM"
     gene            complement(12451..12663)
                     /gene="cspF"
                     /locus_tag="ACHOQQ_RS14395"
                     /old_locus_tag="pgaptmp_002879"
     CDS             complement(12451..12663)
                     /gene="cspF"
                     /locus_tag="ACHOQQ_RS14395"
                     /old_locus_tag="pgaptmp_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HGU4471099.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            12964..13179
                     /gene="cspB"
                     /locus_tag="ACHOQQ_RS14400"
                     /old_locus_tag="pgaptmp_002880"
     CDS             12964..13179
                     /gene="cspB"
                     /locus_tag="ACHOQQ_RS14400"
                     /old_locus_tag="pgaptmp_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HGU4471100.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNFRT
                     LFEGQKVTFSVESGAKGPAAANVIITD"
     gene            complement(13543..13713)
                     /gene="ynfU"
                     /locus_tag="ACHOQQ_RS14405"
                     /old_locus_tag="pgaptmp_002881"
     CDS             complement(13543..13713)
                     /gene="ynfU"
                     /locus_tag="ACHOQQ_RS14405"
                     /old_locus_tag="pgaptmp_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HGU4471101.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
BASE COUNT         3501 a         3121 c         3500 g         3615 t
ORIGIN      
        1 cagatgatta aagctgatta cattgcgata ggtactctac gactaacttt tcaacaagag
       61 taagggggta atcaatttta tttgtatcaa tggtatgaca tttcttggcg ataatgtttt
      121 gatcttatcg attcatgtca tatggtcatt ggtaacgtcc gctcttggta aagagtggac
      181 gctcagatta ggtttggcat ctaagcatag atgtgtcagc tcacatctaa gctaatacat
      241 attactcaat cactcccgca aatctgtaaa tcttgtgtga tgcccattta tttgggcagg
      301 atttaatatc aggatctgga aggtcaggcc tgtatttctg accagttctc ctgtttacgc
      361 tgttccagcg aagaactgtc gatactgaaa caccacagaa atcggcgact tgtttagttg
      421 tcattaagtt gttcattact tcacctcctg cggcggctcc ggtagtggca tccagtgggt
      481 tactttcgat gcctgttctt cctcattgtc agtaactgcc caccatttgt ttctcgacca
      541 atcgtaatac ccttcgaagg tatcgcactc agtccagccg taagacttcc cccaacacca
      601 aacatactgt ttatcattcg gcattcgctc actacagctt atccaaccac ccggacttac
      661 cggagagttg ccagacagcg cgttctgcag ccgttccagc ttaacgtatt cctgaaccct
      721 gtttccgtcg cacacctgaa gccattgcgc agccttttgc gcatcagtgt gaaaggcaca
      781 agtgcgaccg tcatcaaatt gcatttcgta gaggtcagca acttgttcaa actgcgtgtg
      841 tggtgacttg taagtttggt ttacaggttc ggcacaatga agcatggcgg cgcggcaggc
      901 gttccagcct tcatcaaaac cgactatgcc attatttaaa gacggacgag catctggcac
      961 taccggcact ggcttggcta tatatagcgg ctgaacatac cagccctttg ataaccaact
     1021 gtcagcaatg tttttactcc tcgttattgc cggaatacct aagccatttt ctgaatgcag
     1081 ccatgccacc ggctcctctt ctagcgatgc cagagcaatt tcataagcac ggcgctcaat
     1141 atcgtctcga acctctaggc tgctgattcg ttctttgatt tctttaatca gttctttatt
     1201 ggtaaatgtg gtcattatgc tccagcctcc ggtgcttttg gcattactgc ccagtgagtg
     1261 atattgacgt tttcaaggtc cccgacctga aatgtccact gccattctcc ggtttctttt
     1321 tgtccccagg tgtaccagag agaacgccag ccaatcagcc agccttctcc attagcatca
     1381 aataacagaa cactttcatt tgctggtggc agttcagctg acactggtat tattttgttt
     1441 tccagtgccg cacatttagc ttcaagcgcg tcgaatttac gtaccaggta ctcagcattt
     1501 gtttcgttca ctttcagatc tcgcggtaca catttcccgc gaagaaaccc ttccatttcg
     1561 aaaacattca tgcgcatttg cgtaactccg ataaatcgtt aaaacgttcc ataaacatcc
     1621 cgtaggcatg gcccggtgcc agtggaatca cgttgaacat ctctgttgcc gggatgcctt
     1681 ccagtacagg ccagaaagag ccatcatcaa gcccgagatc gcggcgttcg gttgccagca
     1741 tgatgagatc ggcatatttc acgggtgtac tcataaccgg gggtaacccg tatttctcac
     1801 ggattacggc gtctattttt tcttccatcc gtttatagtc aggaagaagg cgtttcagtg
     1861 gtgcgggaat gtcctggcaa tacgcttctg ttgcatcatg cattaacgct tcaaaagcaa
     1921 attcctgcgg caccagtttg ctgcaaagca ccgcatgttg ggcgacgttg tagaagtgcg
     1981 aaagatgacc ggcaaagcga cagatatttg aaagggaaac cgcgatatcg ttaatatcga
     2041 tgtcgtcttt atttatcctg tcataataaa aatgcttccc agaaaaagtt ttgataaatg
     2101 acattttgtt ctccacgtat atgcgctgca ccgcgctgaa ttctggtaaa aagaatcctt
     2161 caccatccgg cgattattga gtaaattacg tttccataaa tgcccccgca ggggcatttg
     2221 cagtaatgaa atcaggcggt gaaagtacca ataaaggttt ctactttgct gtccttgaat
     2281 ttctcaacaa gcagatcacg aaattcgtta gccatttcct cctgcaccgc ctccagctga
     2341 ataatgcgca gaaccagtac aggacgatcg ccagtgataa tgctgaggcg taatttaaac
     2401 ggacgttctt tcagaccttc aaacggaacg catttaaatt caaatgccac tggcataatg
     2461 tctttggtct tcgcttcgac agactccatc agggagcgtt tgccgctgaa gtcattatct
     2521 tcaaaatcag cggtctggtt tgcttcaatc gtgattttac ggaccgccgc agccgctttt
     2581 gttgcctgaa tggcgtcacc attagcatca aagcccacaa ggtagtcggc ccagtcttcg
     2641 atccattctg ccagtgactt ctgggagtta cgctcgccgt taacagacaa cagagcagag
     2701 aactgtgctg tctttttcag tttgagagtg gcagtgttat ctgcgtgacc tggctcatca
     2761 atggtaccca ggttaagcac actgacggca cgcatattat cggcatcgat aaagcagcgg
     2821 gtgccttcat ctgcaagatc tttagaataa cgggtaaagt catcgatgct ggcagtggaa
     2881 agcgcaccac ggaaacggaa gcgatttaaa ttaaattttt ccagatcatg aatgcggaaa
     2941 ttctcaggca atgccacagc atcggcacca atcttactga taatttcatt aacaccctga
     3001 gcagaaataa gggcatggat ttgattaatt gcggttgcgt ctaagttctg agacataata
     3061 agtcctcact atataaagat attcagtgat gagataaata atcagttaat taagaacgat
     3121 attaatgacc tgctgcgcgt agttttccgt caggttcacc ggcaagagtc agtaattgtc
     3181 cctggtcttc ctgcagaata gtcaggcgac caccgcgatt gacatacatc ggcgtttcgg
     3241 tggtgtcttc ttcggaaatt ttcccgcggt tagtcgggcg aacatatgag agtttgtgtt
     3301 tgattttcac acggttctca tcaaatggtt cgatttccag gttgagtgag accttacctt
     3361 tggttttcgt gttcatcaca ccggaagcga cttcactgag aactgcgccg attttggttt
     3421 caaatacgcc gccgtccagc tccccgataa atgcctgcac atcagtactg cgttcgctag
     3481 ccattttgct gctcctcatc atatcgaccc tgcaaggccg gttggtttct ccacaaaaca
     3541 gagaagaaca cctgcggtgg cagccgcccg gatggattgg gttatgagcc cgtcgtccgg
     3601 tgatgctctt ctctgttttg taaaaagagc ggtaccagcc ggaagcaagt gtacaaactg
     3661 gtaccgccaa agcagtggct gttgtggtgg gggtgtcact caggcgtatg gtcaacctga
     3721 caatccggtg tcctcaacgg ggaaagagta acccagccat acttaccgcc gcgccatttc
     3781 gcggatgacc acaacgctga gagcacttag ccagttacgg caccacactt tgtcgcggct
     3841 ccataaatgc cctcatcgtt gcaccctggt ctcttcccag gcgtcaaacc ggatcgccac
     3901 gctggttagg cgtcttatca gcatcatcat tgacttgcac attccggcta cctggtttgt
     3961 ttgcccgagc aaggagtgga ttgtcccctt taacgtcccc agaccgctaa cgacgcatgt
     4021 gccatacgcc gtgttacaac caaattttgt ttgaatcttg cctgcctcat gtttcttttg
     4081 gatacattat gtatctcatg ggtacattgt caagtataaa aaaacctgcc gaagcaggtt
     4141 cataaatatt gattaggcct ttattgtgta tcttcttggt tttcccgaga aaatcactgt
     4201 accaattata gagcaattac cgttaatctt aatgtaaggc tcaggccagt ttgggtttaa
     4261 tgctttgaga taacgctgtg ttccatcttc tatcaatcgc ttgaaggtgg tttcgcctgt
     4321 atcgtgcatc aatgcaataa cgtcgtcacc gtggcaggca gggacttcgg gatcaacaaa
     4381 aatcatgtct ccagggcggt actcatcaat cattgaatca ccaatcaccc gcaagatata
     4441 agtcatttcg ccacagggta cagggcaggg atacgtttct gctgtgctca aatcaacctc
     4501 agaatagcca acttctttcc atgctccggc ctgtacccat gatatgacag ggactaacgt
     4561 tatttgtttg ttagtgattg aaacatcagg tttttttgtg atgttcgttg tctggtgttc
     4621 ttgatcaagc catccgacag gcaggtcgaa acatttttcg atgtgccgtg ccatgctgtc
     4681 accgatattt ttagtagcgc catcccccat aaacctgctg gtctgggttg gctcgcgatc
     4741 aatcatggtg gcaaaggatg aatttccgcc aacaccatct ctcagttttc tggcgttaga
     4801 ccgccggatg tcatggattg ttttcataac gaaattaaaa cctttgtacc gataaggtac
     4861 aagtatcttg aaggttcatt tcaatcatgt aatatgtata tcggaggtac atattgtatg
     4921 aaagcgtatt gggactcttt aaccaaagaa cagcagggcg agttggccgg aaaagttggc
     4981 tcaacacctg gctacttacg gctggttttc aatggttata aaaaagccag ttttgtgctg
     5041 gcgaaaaaac ttgagcaatg cacgtcaggt gcaattacga aatctgactt aagaccggat
     5101 atctatccga aagattaaca gaacaccttc aatttttaac cacagaacga tgaggctaac
     5161 cgtgggtaag tatcactgga aagtagaaaa acagcctgag tggtacgtga aagctgtcag
     5221 aaaaactatc gcagcgttgc cgggtggtta cgctgaagca gctgactggc tggatgtaac
     5281 agagaacgca ttatttaacc gccttcgtgc cgatggcgat cagattttcc cgctgggatg
     5341 ggcaatggtt ttacagcgcg cggctggcac tcactacatt gcggatgctg tcgcacagtc
     5401 tgctggtggg gtgttcgtat cgcttcctga aattgaggaa gtagagaacg ccgatataaa
     5461 ccagcgcctg ctggaagtca tcgaacagat cgggaattac tcaaagcaga ttcgttcggc
     5521 aatcgaagat ggggtcgtgg agccacacga gcagacagca attaatgatg agttgtatct
     5581 gtcaatttcg aagctccaag agcatgcagc actggtctac aaaatctttt gcgctccaga
     5641 aaagagtgac gcccgcgagt gtgcagctcc gggcgtcgtg gcgttttgtg tctgtggaga
     5701 aactaacgca tgaacagttt aacggcaaat aaccgtttgt cgcaacagct ggtggtcagt
     5761 gtcgctgaac acctgttgtt acggcatgaa tgcagattac caaatcacct ggctgtaagt
     5821 aaccacagag aactttacct gactgtgggg ggcgagttgt gcgggaactt aaccgctggt
     5881 ttcgtgacgg aagagggctt tatgtccatg ttattcgttg ggaaccagaa acacagcgcg
     5941 ttatctatct tcgcaaagac tacccgcatg agtgctttag tcctttgtgg aaattcaggc
     6001 gtgattttgt tgagtgtgaa ggaccaccag cacattgatt ctgccattcc gggacgttac
     6061 actgttcagg caccttataa agcgggtgcc gggattggcg tcctggaatt gatcaaggcg
     6121 atatatgacg cgccagcgtc ttttttatcg tccgcatttg ctcacatcaa agttatggtg
     6181 ggctgggcgg gggcatcgaa agatgcgccg gtttccttga tcaccggtta cgccaacccc
     6241 gttcagttca ccaccagcga aattggcgtt tccggtggtg gaagtatttc accgatcaag
     6301 gaggctgcca tcatggctac tgtcccagcc ctcactcgtc tgaatgatga agacttacat
     6361 aaactcagtt atgtaacaac tgcactacgt gctctgcgca aggtaactct ttcggatccg
     6421 caggcgcatc aggttctggt agaaaccctt attaacttgc aggctgaacg tattcgtctg
     6481 gcggataagg ctaattttca tattcaccgt ctcctgaata tcagcggagg gcatcgtcat
     6541 gcttaatccg ttgatcctca acatttaccg tttatttcag cgtaaaaaaa catcaattcc
     6601 tacagttggg cagtggtaca ccacgcctgc agggcatgtt ctacgtgtta gcctggttga
     6661 ccgtgaatgt cagaaggtga tttgtgaacc gctgggccgt aattaccgcg tcagtatgcc
     6721 gcttataacc tttcgctccg gaaaaaacat gaagcatctc ggaggtgcag catgagtatg
     6781 gagctgatgg ttaaagcgat gaaaattcga gtgggtaatc cattgcgaaa actggttctg
     6841 atcaagctgg ctgataatgc cagcgatcag ggtgagtgct ggcccagcta ccagcatatt
     6901 gctgaccagt gcgagattag caaacgttct gtgatgaatc atattgcggc cctttgtgag
     6961 tccgggctgg taaaaaaagt cacccggaaa ggtgaaaaag gtaactcaag taatatctat
     7021 ctccttcatc tggatggtgc aggagattca ctagggggta gtgcaaataa ttcactatct
     7081 ggtgcagcaa attcaccagg tagtgcagga gttgcaccag ggggtagtgc aggagattca
     7141 cccagaacca gtcactcttt tgaaccagtc aaagaaccag tcaatgaacc aatagctgtt
     7201 ggtgcatcag ttgatgagtc cgtgcgagtt cgttcaaacc gaccggaata ttctccggag
     7261 tttgagcagg catggctggc atatcccaaa cgagctggtg gcaattcaaa atctgcagcc
     7321 ttcaaagcct ggaaagcccg tttgaatgag ggggtaaacc ccgaaaccat gctggaaggt
     7381 gtgaaacgct atgcgggctg ggtatctgcg atgggtaaca gcggcacaca atttgtgaaa
     7441 caggctgtca cgttctttgg tccggatcgt catttcgaag aatcctggga agttcctgcg
     7501 gtatctgcag ccagacgtga ggacccgtac ttcaaatcca gttacgacaa cgtggactac
     7561 agccagatcc cggcaggatt caggtggtga tcatgagtct tttgaatgac gttcagaaat
     7621 tcattgaagc ccatccgggg tgtacttccg gagacattgc ggatgctttt gcaggttact
     7681 cacggcagcg cgtactgcag tcagcaagca agttacgtca gagtgggcgt gtggctcacc
     7741 gttgtgaagg ggatacacgc agacatttcc cgcgcctgac tgagagagcg caggagccgg
     7801 aaccacaacc agttcgtgaa accagacctg tgcgcaattt ctatgtcggc actaacgatc
     7861 cccgggtgat tttgtgcctg acccgccagg ctgaagaact ggagtcaaga ggcttatacc
     7921 gtcgtgctgc aaccgtgtgg atggaggcat tccgtgaaag ccactcccag ccagaacgaa
     7981 acaattttct ggcgcgtcgt gaacggtgtt tacggaaaag cagcaagcgg gctgcatcag
     8041 gtgaagagtg gtatctgtca gggaattacg tgggggctta atgagtaata aatattgcca
     8101 ggcgctggtg gaactgcgga acaaaccagc ccatgaactg aaggaagtgg gcgatcagtg
     8161 gcgcacgccg gacaacattt tctggggaat taacaccctg tttggcccgt ttgttctgga
     8221 tctgttcacc gacggtgata acgccaaatg tgccgcgtat tacacggcgg aagacaacgc
     8281 gctggcgcat gactggtcag aacgtcttgc ggagcttaaa ggtgctgcct ttggtaatcc
     8341 cccatacagc cgcgccagtc agcatgaggg gcaatacatc accggcatgc gttacatcat
     8401 gaaacatgcc agtgccatgc gtgataaagg cgggcgctat gttttcctga tcaaagctgc
     8461 caccagcgaa gtgtggtggc cggaagatgc agaccatatt gcttttattc gcgggcgtat
     8521 tggttttgaa ctgcctgcct ggtttatccc gaaagacgag aagcaggtgc cgacaggcgc
     8581 tttcttcgct ggtgctattg ctgttttcga taagacctgg aagggaacgg caatcagcta
     8641 catcgggcgc gatgaacttg aggcatgtgg tgaggcgttt ctggcgcagg ttcgccagca
     8701 ggcggaaaaa ctggtcaggg agatggcggc atgacgacat taactcaatg ccagcagcag
     8761 gtgctggata tgctgatttc ttaccagaaa gaacgtggct tcccgccaac caatcaggag
     8821 gtggcaacca tgctgggata ccgttcagtg aatgcagcgg tggagcatct tcgcgcactg
     8881 gagaaaaaag gcgtcatcac gataaagcgt ggcgtggccc gggggataac gcttcatacc
     8941 gcggtgaagg acgacgacag cgaggcggtc gggattatcc gctcactgct tgccggtgag
     9001 gaaaacgcaa ggctgcgtgc agcccactgg ttacatgaga ggggcctgaa agcatgaagc
     9061 tgatcctgcc tttcccgccc agcgtgaaca cttactggcg acaccctaac aaaggggcgt
     9121 ttgcaggtaa gagcctgata agcgcggcgg ggcgaaaatt ccagagcgcg gcgtgcgcag
     9181 caatagttga gcagttacgt cgtctgccga aaccaacgtc ggcacctgct tcagtggaga
     9241 tcgtgttgtt tcctccggat aacaggatcc gcgatctgga taactataac aaggcgctgt
     9301 ttgacgccct gacccacgca ggtgtgtggg aagacgacag ccaggtgaaa agaatgctgg
     9361 tggagtgggg accggttatc ccggaaggga aggtcgagat cactatcagt aagtacgaga
     9421 aaacggcggg tgcagccgcc tgattaagag gagaaacgaa gtatgaataa tctgatggtc
     9481 attgatggta ttgaagttcg tcgtgatgct tatggacgtt acagcctgaa cgatctgcat
     9541 cgtgccgcag tagcatctgg tgcaaatgcc agaacgaagg agccgggaaa gtttctttcc
     9601 agccaacaga ctgttgaact tgttcaggaa ttgaccgata cccaaaattt gggtgtcggt
     9661 ccggttaata cagttcgtgg tggtcctaag caggggagtt atgtctgcaa ggaactggtg
     9721 tatgcctatg caatgtggat cagtccgtca ttccatctga aggtgatccg tactttcgat
     9781 atggtagtca gtgcaccgga aaaattatcc gggcaggctg ctgacaagat gcaggcaggt
     9841 gtgattctgc tggactttat gcgtcgggag ttaaacctgt ctaactcatc tgtgcttggg
     9901 gcctgtcaga aactccagga ggctgttggc ttaccgaatc tggcaccgcg ctatgccatt
     9961 gatgctcctg ctgatgcaca cgatggctca agtcgcccca cgctgtcgct gagtgcactg
    10021 ctgaggcagt atggtatccg cctgacggct aatcaggcat atcaccagat ggtgaagctg
    10081 gggatcgttg aacaacgcga acgatacagc cgtaccgcga ttaacaacat caaaaaattc
    10141 tggtcgctga cggcgaaagg ctgcatgttc ggcaagaaca tcaccagtcc tgcaaatccg
    10201 cgcgagacgc agccgcattt cttcgaatcc cgattccctg agctgttaaa gctgctcgat
    10261 accgttcatt gaggtgaccg tgagagcact actgacccct gaaattgccc cgcgtatggg
    10321 gatcgtattg ttcaggccag gttcagagct gatgcccctg tttatgcagg ggcgtgtcct
    10381 gctggagcct gagccggaac gttattcatc tttcgccagt ggtgccgttc cggcggcatc
    10441 acaaccgctg gcggatgatc ctgccgttcg ggccgtgttc cgcaatgagg cagtgatccg
    10501 tcgtgctggt ggcgtggaat gtcttgaaag ctggttactt cgtgaaaagg gctgtcagtg
    10561 gcctcattcc gactggcaca gcgagaacat gaccacaatg cggcacgctc cgggagcaat
    10621 tcgtctgtgc tggcactgcg ataaccagct gcgtgaccag ttcacggaac ggctggaatc
    10681 aatggcaacg gataactgtg cccgctgggt gttgtctgtc gtgcgtcggg atctcggttt
    10741 tgatgacagt cacgttgtga caatgccgga actgtgctgg tggctggttc gtaatgattt
    10801 ggcggatgcc ttaccggaaa gtgcagcccg taaggcactg agattaccga agcctgttgt
    10861 gccatctgtc acccgggaaa gtgaccttgt gccttcggtt cctgccacca gcatcatcca
    10921 ggataaagcg aaaaaggtgc tggtgctgaa agtggatccg gagtcgccgg agtcttttat
    10981 gttacgcccc aaacgtcgcc gctgggttaa tgaaaagtac acgcgctggg ttaagacgca
    11041 gccgtgcgca tgttgtggaa agccagctga tgatccccac cacctgatag gccacggtca
    11101 gggtggaatg ggtacaaaag cgcatgatct ctttgtgttg cctttgtgca gaaagcatca
    11161 cgacgagctg catgcggata ccgtgacatt tgaagagaag tatggctccc aactggagct
    11221 gatatttcgt tttatcgatc gtgcgctggc aattggcgta ctggcgtaag tggagaacga
    11281 gcatgaacct tgaagcctta ccaaaatatt actccccaaa atctccaaaa ttgagtgatg
    11341 ccgcaccggc gacaggctca ggtggtttaa cgattacgga tgtgatggct gcgcagggga
    11401 tggtgcagtc gaaagcaccg cttgggtttg ccttattcct ggcaaaagct ggcgttcagg
    11461 atcctcagtt tgcgattgaa ggtctgctca attacgcgat ggcactggat aacccgacat
    11521 tgaacaaatt gagtgaagaa atccggttac agatcatccc ttaccttgtg agttttgcct
    11581 ttgctgatta ttccaggtct gcggcaagta aggctcgctg tgagccttgt gctggtactg
    11641 gatttcataa tgtattgcgc gaagtggtga aatactccag aagcggggag tctgttatca
    11701 aggaagagtg ggtgaaggaa ctatgtcagc attgtcatgg taagggagaa gtcagcacag
    11761 cgtgcagagg gtgtaagggt aaaggtattg tcctggatga aaaaaggacc cggcttcatg
    11821 gcgcgcctgt ttataagatt tgtgggcgtt gcaatggaaa ccggtttagt cgtttaccaa
    11881 cgacactggc gcggtgtcat gtccagaagc tggtaccaga cctgactgat tatcagtggt
    11941 acaaaggata tgcagatgtc attgataaac tggttacaaa gtgctggcag gaagaagcat
    12001 atgctgaggt gcaattaaga aaagtgacga gataaatgat tttcgccgaa gatggcgacg
    12061 taatgcttgc atttttcaaa aaatatggat aaaattcttt caacgatggg ctttatatac
    12121 ccgacgttaa gaaaaagtag aaaacccgct gatgagcggg tttttgtgct ttaaataggg
    12181 taatagagat gttgaatctc attccgggat tcatgtttgt ttacttatta tttatcgggt
    12241 gactttgcct gatgtttaaa atgttttctt ccagtgctat gtccctagat acaatgagtc
    12301 tgcatattac attattagca gagctattac ggtcaaagta cagcataagc ttttaaagcc
    12361 aatcaaccag tcatcaagac agacggggtt attcatgaaa actctccatg tttgattcga
    12421 tggggcctga aattaaagct ttaatatagc tcatgaaagg taaacattgg cagctgaagg
    12481 gccacgcaga ccatttatcc ggcaaaattc cacgcgtaat ccggtggtaa tttcttctgc
    12541 atcgcggaga ttgagcgctg aaacatgaag ctggacatcg atacgaccat cggatggggt
    12601 gataagaccc ttgccgcttt tgccgtcaaa ggttttgaca attcctgtca ttttacggga
    12661 caaaaaaatt ccttaatact gataactggg cgcactatac acacgttcct gaagaaagct
    12721 atagtttttt gatggggttg aagatggctg gatgtctaaa ataaacattg cttcatatgt
    12781 tcaactatgc gttaatgatt gcgtcggttt gaagaacaga cgatatacga agtagtttac
    12841 taaagcagtt ctcatttcag gtgttattca cttattcctt ctttgagtct ctccaattaa
    12901 gtacgaagtc gtttctgtta tacaagccat ttatgccgaa aggctcaagt taaggaatgt
    12961 agaatgtcaa ataaaatgac tggtttagta aaatggttta acgctgataa aggttttggc
    13021 tttatttctc ctgttgatgg tagtaaagat gtgtttgtgc atttttctgc gattcagaat
    13081 gataattttc gaaccttatt tgaaggtcaa aaggttacct tctctgtaga gagtggtgct
    13141 aaaggtcctg cagcagcaaa tgtcataatt actgattaaa attcatagtt tgtctgtata
    13201 cgataacgaa gaaggctgat gcctgagtgg agatacagac agagtggtga atattggatc
    13261 tctttaataa atagtaagga ggtccaatac atgaaacaat ggccagcata tttggcaata
    13321 acttaatcag gaaaagtatg ctaaccattg tggtgaagtg caggtttgct gcatgaatag
    13381 ttttacagca gaagctaact gctggcatag caaaacaaag tgcgtaagtg gatgactccc
    13441 acaaaaagta ccacaatatt aaacccgctc aggcgggttt tttattatct gctttaaata
    13501 tgttattaaa atataaaaaa tacttgttac gaataaaatc aatcaagcta cagctttaag
    13561 atttgtctgg aatactttgt tgcaatgagg gcagatcaaa agggcacctt tttgtactct
    13621 tgaaaaactg tgttctgact cttgggtgca gtttgggcag gaacatttaa cgagataatt
    13681 acggcgtgat tttgagtctt tacgttctga cataggcttt tcctgtataa atggccg
//
LOCUS       DAZKFU020000122        13649 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZKFU020000122 DAZKFU020000000
VERSION     DAZKFU020000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.123"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..849
                     /gene="ydjI"
                     /locus_tag="ACHOQQ_RS14410"
                     /old_locus_tag="pgaptmp_002882"
     CDS             13..849
                     /gene="ydjI"
                     /locus_tag="ACHOQQ_RS14410"
                     /old_locus_tag="pgaptmp_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HGU4471102.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            854..1801
                     /gene="ydjH"
                     /locus_tag="ACHOQQ_RS14415"
                     /old_locus_tag="pgaptmp_002883"
     CDS             854..1801
                     /gene="ydjH"
                     /locus_tag="ACHOQQ_RS14415"
                     /old_locus_tag="pgaptmp_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4471103.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            1811..2791
                     /gene="ydjG"
                     /locus_tag="ACHOQQ_RS14420"
                     /old_locus_tag="pgaptmp_002884"
     CDS             1811..2791
                     /gene="ydjG"
                     /locus_tag="ACHOQQ_RS14420"
                     /old_locus_tag="pgaptmp_002884"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HGU4471104.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGALDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            2928..3686
                     /gene="ydjF"
                     /locus_tag="ACHOQQ_RS14425"
                     /old_locus_tag="pgaptmp_002885"
     CDS             2928..3686
                     /gene="ydjF"
                     /locus_tag="ACHOQQ_RS14425"
                     /old_locus_tag="pgaptmp_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4471105.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            3804..5162
                     /gene="ydjE"
                     /locus_tag="ACHOQQ_RS14430"
                     /old_locus_tag="pgaptmp_002886"
     CDS             3804..5162
                     /gene="ydjE"
                     /locus_tag="ACHOQQ_RS14430"
                     /old_locus_tag="pgaptmp_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4471106.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVNVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSIFMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     ALGRIVAVFTPYGVAALLTHYGSITVFIVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(5255..5896)
                     /gene="pncA"
                     /locus_tag="ACHOQQ_RS14435"
                     /old_locus_tag="pgaptmp_002887"
     CDS             complement(5255..5896)
                     /gene="pncA"
                     /locus_tag="ACHOQQ_RS14435"
                     /old_locus_tag="pgaptmp_002887"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGU4471107.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(5907..6923)
                     /gene="ansA"
                     /locus_tag="ACHOQQ_RS14440"
                     /old_locus_tag="pgaptmp_002888"
     CDS             complement(5907..6923)
                     /gene="ansA"
                     /locus_tag="ACHOQQ_RS14440"
                     /old_locus_tag="pgaptmp_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGU4471108.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(7055..7765)
                     /locus_tag="ACHOQQ_RS14445"
                     /old_locus_tag="pgaptmp_002889"
     CDS             complement(7055..7765)
                     /locus_tag="ACHOQQ_RS14445"
                     /old_locus_tag="pgaptmp_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HGU4471109.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEDAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            complement(7881..9737)
                     /gene="sppA"
                     /locus_tag="ACHOQQ_RS14450"
                     /old_locus_tag="pgaptmp_002890"
     CDS             complement(7881..9737)
                     /gene="sppA"
                     /locus_tag="ACHOQQ_RS14450"
                     /old_locus_tag="pgaptmp_002890"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGU4471110.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GPIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            9898..10449
                     /gene="ydjA"
                     /locus_tag="ACHOQQ_RS14455"
                     /old_locus_tag="pgaptmp_002891"
     CDS             9898..10449
                     /gene="ydjA"
                     /locus_tag="ACHOQQ_RS14455"
                     /old_locus_tag="pgaptmp_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGU4471111.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQELSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            10566..11609
                     /gene="selD"
                     /locus_tag="ACHOQQ_RS14460"
                     /old_locus_tag="pgaptmp_002892"
     CDS             10566..11609
                     /gene="selD"
                     /locus_tag="ACHOQQ_RS14460"
                     /old_locus_tag="pgaptmp_002892"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGU4471112.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            11614..13575
                     /gene="topB"
                     /locus_tag="ACHOQQ_RS14465"
                     /old_locus_tag="pgaptmp_002893"
     CDS             11614..13575
                     /gene="topB"
                     /locus_tag="ACHOQQ_RS14465"
                     /old_locus_tag="pgaptmp_002893"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGU4471113.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFSPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
BASE COUNT         3428 a         3199 c         3573 g         3449 t
ORIGIN      
        1 ggagaacaga acatgctcgc agatatcaga tattgggaaa acgatgccac aaataaacgt
       61 tatgctattg cccattttaa tgtatggaat gcagaaatgt tgatgggcgt tatcgacgca
      121 gccgaagaag cgaaatcacc ggttattatt tcttttggta caggttttgt cggcaacacc
      181 tcatttgaag atttctctca catgatggta tcaatggcac gaaaagcaac ggtgccggta
      241 ataactcatt gggatcatgg tcggagtatg gagattattc ataacgcctg gactcatggt
      301 atgaattcat taatgcgtga tgcttccgca tttgatttcg aagaaaatat tcgcttaacc
      361 aaagaggctg tcgatttctt ccatccgctg ggtattccgg tagaggcgga attagggcat
      421 gtcggtaatg aaaccgttta tgaggaagcg ctggcgggct atcactatac cgatcctgac
      481 caggcggctg aatttgttga acgtacgggc tgtgactcat tggctgtcgc catcggcaac
      541 cagcatgggg tttatacgtc agagccacaa ttgaactttg aggtcgtcaa acgcgtacgc
      601 gatgccgtgt ctgttccgct ggttttgcat ggcgcatcgg ggatcagtga tgccgacatt
      661 aaaactgcaa tttcgttggg tatcgcgaaa atcaacatcc atacggagct ctgtcaggcc
      721 gcaatggtcg cagtgaaaga aaaccaggat cagcctttcc tgcatttaga acgtgaagta
      781 cgtaaagctg taaaagaacg ggcattggaa aaaattaaac tgttcggttc tgatggcaaa
      841 gcggaataat gatatggata atctcgacgt tatttgtata ggtgccgcta ttgttgatat
      901 tccattgcaa ccggtcagta aaaatatctt tgatgtggat tcttatcctc ttgaaagaat
      961 cgcaatgacc accgggggtg atgcaataaa tgaagcaaca attatttctc gtctgggcca
     1021 tcgtacagca ttaatgagcc gtattggtaa agatgccgca gggcagttta ttctcgacca
     1081 ttgccgcaaa gagaatattg atattcaaag cctgaagcag gacgtcaaca tagatacctc
     1141 tattaatgtt ggactggtga cggaggatgg cgagcggacg tttgtcacaa accgtaacgg
     1201 cagtctgtgg aaattgaata ttgacgatgt tgattttgcg cggttttccc aggcgaaatt
     1261 attatcactg gccagtattt tcaacagtcc actattggat ggtaaagcgc taacagaaat
     1321 ttttacgcaa gctaaagccc ggcagatgat tatctgtgcc gatatgatca aaccgcggtt
     1381 gaatgaaacg ctggatgata tttgcgaagc attgagttat gtcgattatc tgtttcctaa
     1441 ttatgccgag gcaaaattac tcaccgggaa agagacactg gatgaaattg ctgactgctt
     1501 tcttgcgtgc ggcgtaaaaa cggtggtgat taaaacgggt aaagacggct gctttatcaa
     1561 gcgtggtgac atgacgatga aggtgccggc ggtcgcagga ataaccgcca tcgacaccat
     1621 tggcgcgggc gataactttg cttcaggttt tattgcggca ctgttagaag gaaaaaatct
     1681 gcgtgaatgc gcacgctttg ccaatgcaac ggcggctatc tcggttctaa gcgtcggtgc
     1741 caccaccggc gtaaaaaaca gaaagctggt cgaacaattg ctggaagaat acgaaggata
     1801 atgaaggcaa atgaaaaaga tacctttagg cacaacggat attacgcttt cgcgaatggg
     1861 gttggggaca tgggccattg gcggcggtcc tgcatggaat ggcgctctcg atcggcaaat
     1921 atgtattgat acgattcttg aagcccatcg ttgcggcatt aacctgattg atactgcacc
     1981 aggatataac tttggcaata gtgaagttat cgtcggtcag gcgttaaaaa aattgccccg
     2041 tgaacaggtt gtagtagaaa ccaaatgcgg cattgtctgg gaacgaaaag gaagtttatt
     2101 caacaaagtt ggcgatcggc agttgtataa aaacctttcc ccggaatcta tccgcgaaga
     2161 ggtagaagcc agcttgcaac gtctgggtat tgattacatc gatatctaca tgacgcactg
     2221 gcagtcggtg ccgccatttt ttacgccgat cgctgaaact gtcgcagtgc ttaatgagtt
     2281 aaaggctgaa gggaaaattc gcgctatagg cgctgctaac gtcgatgctg accatatccg
     2341 cgagtatctg caatatggtg aactggatat tattcaggcg aaatacagta tcctcgaccg
     2401 ggcaatggaa aacgaactgc tgccgctatg tcgtgataat ggcattgtgg ttcaggttta
     2461 ttccccgcta gagcagggat tgttgaccgg caccatcact cgtgattacg ttccgggcgg
     2521 cgctcgggca aataaagtct ggttccagcg tgaaaacatg ctgaaagtga ttgatatgct
     2581 tgaacagtgg cagccacttt gtgctcgtta tcagtgcaca attcccactc tggcactggc
     2641 gtggatatta aaacagagtg atttaatctc cattcttagt ggggctactg caccggaaca
     2701 ggtacgcgaa aatgtcgcgg cactgaatat caacttatcg gatgcagacg caacattgat
     2761 gagggaaatg gcagaggccc tggagcgtta aatactcaac acgattcacc tctgtttctg
     2821 tgaaaactgt gatgaaaatc tgtgattcaa acaggttatt ttgaaagtaa acatcggtta
     2881 tgcgataatc gcgctaatgt gatgtaaaaa gatacagggg tgtaatcgtg gcggcaaaag
     2941 acaggattca ggcaattaag caaatggttg ccaacgataa aaaggtgaca gtctcaaatt
     3001 tgagtgggat ttttcaggta accgaagaaa ccattcgccg cgatcttgag aagctggaag
     3061 atgaaggctt tttgaccaga acctatggtg gtgctgtttt aaatacagcg atgttgacgg
     3121 agaatatcca tttttataag cgcgcttcat cgttttatga agagaagcag cttattgcac
     3181 gcaaggcact accctttatc gacaataaaa ccaccatggc agccgattcc agtagtaccg
     3241 taatggaatt gctcaaattg ttacaggacc gtagtgacct gacgttgcta accaactcgg
     3301 cggaagcgat tcatgttctg gctcagtcag aaattaaagt cgtttcaacg ggtggggaac
     3361 taaacaaaaa tacactttca ctgcaaggaa gaattactaa agagatcatc agccgctatc
     3421 atgtcgatat catggtaatg agttgtaaag gtcttgatat taacagcggc gcgctggact
     3481 ctaatgaagc ggaagctgaa atcaaaaaga caatgatccg tcaggcgaca gaagttgcgt
     3541 tattggttga tcactctaag tttgatcgca aagcttttgt ccagttagct gactttagtc
     3601 atattaatta cataataact gataaatcac cgggtgcaga gtggatagca ttttgcaaag
     3661 acaataatat ccagctggtg tggtaatttt agcacacgtt gtcaatatta tttgttcctg
     3721 tgcctggaaa taaaatgatg ataaagatgc ggcaagtgaa tttgccgcaa ttgctacctt
     3781 ttttagcaaa tccgaggcac ccaatggaac aatatgatca aattggcgca agactggacc
     3841 gcttgccttt ggcccggttt cattatcgta tatttggtat aataagcttt agtctgttat
     3901 taacggggtt tttgagttac tcaggtaatg tcgtcttagc aaagctggta agcaatggat
     3961 ggtcaaataa tttcctcaat gccgccttta cctcggcatt aatgtttggt tatttcatcg
     4021 gctctcttac cggtgggttc atcggtgatt actttgggcg gcgcagggcg tttcgcataa
     4081 atcttctcat cgtcggtatt gctgcaacag gggccgcttt tgtccctgat atgtactggc
     4141 tcatcttctt tcgcttcctg atgggaacag gaatgggggc gctgattatg gttggctatg
     4201 cctcatttac ggagtttatc cccgcgacgg tgcgtggaaa atggtccgcg cggctctcat
     4261 ttgttggtaa ctggtcgccc atgctgtctg cggcgatagg tgtggtggtt atcgcttttt
     4321 ttagttggcg gataatgttt ctgttgggtg gtattggcat actgttagcc tggtttctct
     4381 caggtaaata ctttatcgag tcgccacgat ggctggcagg gaaagggcaa atcgccggtg
     4441 cagaaagcca acttcgtgaa gtagagcagc aaattgaaag agagaagagt attcgtttac
     4501 ccccgcttac ttcgtatcag agcaacagca aggttaacgt aatcaagggt actttctggc
     4561 tcctgtttaa aggggaaatg ttacgacgta cattagttgc gataactgtt ttaattgcaa
     4621 tgaacatttc gctttatacc attaccgtat ggataccgac catatttgtt aactccggca
     4681 ttgatgtcga taaatcaata ttcatgaccg ctgttattat gattggcgct ccggtaggaa
     4741 tatttattgc ggcattaatt attgatcatt ttcctcgtcg attatttggc tccgccttac
     4801 ttattattat tgctgtgtta ggctatatct attcaattca gactacagag tgggcgattt
     4861 taatctatgg tctggtgatg atcttctttt tatacatgta tgtttgcttt gcgtcggcgg
     4921 tttatatccc ggaactttgg ccaacgcatt tacgcctgcg tggttcgggt ttcgttaatg
     4981 ccctcggccg gatcgtcgca gtcttcacgc cctatggcgt tgcggcatta ttaacacatt
     5041 atgggtcaat cacggtgttt attgtgcttg gtgtgatgtt agtgctctgc gcgctggttc
     5101 tctccatttt tggcatcgaa acgcggaagg tgtcgctgga agagatttct gaggtgaact
     5161 aaatctttcg ttgatggcgt ccattaccag atgtaccact gacgtgtttt atctggccta
     5221 caatgccagt acagaactgt aggccagcgt aaaattaccc ctgcgtctct tcccagtctg
     5281 ccagcgtata tagcgttgcc ccagctgctg acatctccat aaacgcgtgt gcactgtcct
     5341 gtggctgaat attcacgcca cgacaaccat cggtaatcac gttcacctta taacctaact
     5401 gtaacgcatc cagcacggta aacttcacgc aatagtcagt agccaggccc ataacgatca
     5461 attcatcgat ttcatgatcg cgtaaccagt catcgagcgt ggttttctgc cgacggccgt
     5521 tatcaaaaaa ggcactgtaa ctgtcaacta aaggattttc gcctttatgg aacaccgctg
     5581 cgatcgcttt ttggttcagt aacggatgta attgtgcgcc ttcactgttc tgcacacagt
     5641 gatctggcca gaaggtttgt ggcaaaccgt cgagttggcc tggcgtataa ggctctacac
     5701 cgtgctgact ggcaaaactg ccgtgattcg ccgggtgcca gtcctgactg gcgataaccg
     5761 cttcaccgcg cgactggcac cagtcaatca ggcggttagc gacatccacc gtactgtcac
     5821 cttccggcac ggcgagcgcg ccaccagcac agaaatcatt ttgtaaatcg accagtaaca
     5881 gggcgcgagg gggcattgca gtctccttaa tcatccggcg tcagttcgcc gcgcaggttt
     5941 tggctcatgg ccttgcgaat ggtttcagta tccagttcct ggctaagcag gtaatgcagt
     6001 ttggttagcg tggcttctac agtcatatct gcaccgccaa taacgccagc atgggcgagg
     6061 gcgttaccgg tggcataacc acccatattc actttaccgg acatacattg tgtcaggttg
     6121 accactacaa taccgcgatc gctggcttct tgtaattcct gcaggaaggc tttgttttgt
     6181 ggcgcattac ccacgccata ggagcgcaga atcaatgctt tcaccggttg gcgcagaaaa
     6241 ttgcgcacga cgtcagcaga aatccctgga taaatcgtca ctacgccaat tggttgtggg
     6301 gtgattggat gaacgattaa ttccccttca ccgtgcgggg cgggtggcgt attcaaacga
     6361 cgaatatgga tacctgcttc cagtaacgga ggaaggtttg gagaggcaaa cgcatcaaaa
     6421 ccatcggcat gggctttggt agtgcggttg ccgcgataca gtcggttatt aaaaaagagt
     6481 gttacttcgt tgatcggata attcgccgca acgtacaacg cattcagcag attaatttgt
     6541 ccgtcagagc gtaactcagc cagcgggatt tgtgaccctg tcacaatgac cggtttaccg
     6601 agattttcga gcatgaacga cagcgcagag gcggtatacg ccatcgtgtc ggtgccgtgc
     6661 agaatgacaa aaccatcata gtcgtcatag tgcgctttaa tatcttcagc aatatgctgc
     6721 cagtcttccg gcgtcatatc tgaagaatcc atcagcggcg tatattcatg aatggtgaaa
     6781 tctggcatct ccgggcgatg gaattccggc atcagcgcca gttggcgttg tagatgacct
     6841 gacaccggta tataaccctg ctcggaacgc tgcatcccga tggtcccgcc cgtgtaggca
     6901 acgtaaattg atttcttttg catgatattg atatgaatga tatcgaaaga gcaaaagtat
     6961 atacgtgagg ggcagcgagc gcacctggat actctgtgga tatgagaaaa gtccggcagg
     7021 acggagggat tagtgtcccg ccggagaaaa gtggttaatc ctgcaacgca taggttacgg
     7081 tcgtgactac gcgtaccgtt ttattaatcg ccgtaggatc atcttcatca ctgccactgg
     7141 caaagatctg gaatacgcct tgtgaggcat cgctaattga ccccacctgg ctacctgagt
     7201 ccgttgcaaa ttgtagtgcg gcgttgcgtg catctttggt ggcggcagca atcatctcag
     7261 cgcgcagtgc gccaatacca ttaaagcgat aggcgacttt gctctcagcg atagcgccat
     7321 ccgtagcgac ttgcagttcg tttaatttcg cgacgacttt ttcgacagca gcaacattat
     7381 gggtttttat cgtcactgac ccgtctgcgt tatagcgtgg cattgtggga tcatctttca
     7441 gatagaagga atctgttcgc ttgcttgtcc attggcccag gattatctct ttctcgttaa
     7501 tcccctgagc attgagttcg gcaacaattt ttttctgcgc gtcttccagt ttcgggaaca
     7561 gttcgccggg aacattaccc ttaaagttaa tcgcaattgt taattctgcc gtatctgcgc
     7621 gaacttcttt ttcagaaagc ccgcgcacat tcacataacg gttgttggtt tttaaatgtt
     7681 taacaccatc gccaacaaaa taaccacagg cgctaatccc cagtccgagg acgatagcgg
     7741 caattaaaga gggggccttg ttcactgatg actccatatc agatgctaaa tgagcaaggg
     7801 aatgtagtgg agagagggag tgaaagacag tccgattcag gagtgtggcc gacagcatat
     7861 cgaccacatt tttgcaggat ttaacgcacg ttggcgcagg tcaggcaaaa cgcataacgg
     7921 ttttgtgggt cgttaaacgc ggccagcttg tcactttcac ttttaacggt agaggctacc
     7981 gaggcaagcg gtgcaggtaa catggcctgg aacgcatctg gcaacattgc ccggacagaa
     8041 ccagacatgt tgtccatcac tttgtcgaag aaggtcggtt catcaacgta gtattccaga
     8101 tgccactgtt tcacttttgc cagctctgct gctttggcga ccgcatcatc gaaatccccg
     8161 agactatcga ccagcccgtt agcttttgcg tcctgaccgg tccagacgtg gccctgggcg
     8221 attttatcaa tctgctccgg cgtcgaatga cgcgcatcag caaccagcgt aataaagcgt
     8281 ttatagccat tctcaatgct taactgcatc atctgctgcg cttccggcgg cagtgccctg
     8341 gtgatagaaa catctgccag cggtgaagtt gagacaccat cagtatgaac accaatcgaa
     8401 tccagactat tttctacggt ggtgatcacg ccgaagatac cgatagaacc ggtcagggtg
     8461 ctggggttag ccacaatgta attagctggc gtagaaatcc agtaaccacc agatgccgcc
     8521 atgccgccca tcgatacaac cacaggctta cccgctgccc gggctgctgc cagttcagcg
     8581 cgaatcactt cagacgcggt aacgctgccg cctgggctat taacacgcag gacaatcgct
     8641 ttcactttcg ggtcaaggcg agcgtcgcgg atttgtgccg cagtggtatc accgccaaca
     8701 ttcccctgag tttcctcgcc atccataatt gggccattag cgaagacgac accgatgctg
     8761 tcaccggtat ctgccggcgt tttcaatgcg taatcgtaat aactgatggc gcgataattt
     8821 ttatcagtct tactccagcc gaactctttg gtcagagctt tttcgatttc cgcactcgat
     8881 gccagtgcat cgaccagctt gttttccagt gcatatttcg cggtatcgcc accggttttg
     8941 gttaaaccct caagcaaccc ttgcgcgcca gggaatacct gctgagcagg gatctgccgg
     9001 ttagcggcaa cagtattcag atagttttgc cacagctcac caatccagcg gctgtcagct
     9061 tcgcgggctg ccggtgacat gtcatcacga ataaacggtt caacggcaga tttatacgta
     9121 cccacgcgga acacatgggt ggaaactttc agcttatcca gcaacgattt gtagtacaga
     9181 ccgttggtag caaaaccgtg caaatccacc acgccttgcg gtgacagcca aattttattg
     9241 gcgaaactgg cgagataata ttgcccctgg ctgtagttct cgccaacggc ataaaccggt
     9301 ttcccgctgt cacgaaactc tttcagggct ttgccgatgt actgcataga cggctggtcg
     9361 ccgcctgcga agtttttcag atccatcacg ataccggtga tattgcggtc gtccttcgcc
     9421 tggcgaatgg tgttgacgat atcaaacagt gagttttcct gcagacgatc ggaactggca
     9481 ccaagtagtt ggcggcttaa tttactaaac cgctgagaac tgtcgggttt atcgacgatt
     9541 acaccagaaa tgtccagcag cagtgcacca cgactggccg tttctttcga atcaccacca
     9601 ctgacctgca tccagatccc cacaccaacc agcacgagaa aaataaagaa caggttaagt
     9661 accatttcac ggacgaaatt cagcagacgc cacgtccatt taaaaaatcc ggcaataaat
     9721 cgccaaaggg ttcgcatgta ttctcccaac ttaaggtcac acctgtcaca ggcgcaatag
     9781 atgtggttat cgtaatgacc caaccgacac ttgtcagcag gaattgcgga ctgtgctgta
     9841 acaaaatcca ctctcgtgtt aattttgtga ataaatatca cgacaggagt taatcaaatg
     9901 gatgcactcg aactattgat caatcgccgt agcgcctccc gcctggctga acccgcacca
     9961 acgggtgaac aactgcaaaa catcctgcgt gcgggtatgc gcgcgccgga ccataagtcc
    10021 atgcaaccgt ggcatttttt tgtgattgaa ggggaagggc gcgagcgttt cagcgccgta
    10081 ctggaacagg gggcgattgc tgccggtagt gatgacaaag ctatcgacaa agcccgtaat
    10141 gcgccgttcc gcgcaccgct catcatcacg gtggtggcga aatgcgaaga aaatcataaa
    10201 gtcccgcgat gggaacagga attgtctgcc ggatgcgcgg tcatggcgat gcaaatggca
    10261 gccgttgccc aggggtttgg cggcatctgg cgcagtggcg cattaactga aagtccggta
    10321 gtgcgtgaag cattcggttg ccgtgagcag gataaaattg tcggttttct ctacctcggt
    10381 acgccgcagc tgaaagcatc tacgtcgatt aacgtcccgg acccgacgcc gtttgtcact
    10441 tatttctgat aattgtcgcg aaactgtctg gattgtgagc aaaggcgcag gaattcagac
    10501 actctcactt atcacttcac ggaatgaggg ttaccatacc cggattgcat tgacaggaga
    10561 tgtccatgag cgagaactcg attcgtttga cccaatacag ccacggagct ggttgcggct
    10621 gtaaaatttc cccaaaagtg ttggaaacca tcctgcacag tgagcaggcg aagtttgttg
    10681 atccgaattt gcttgtgggt aatgaaaccc gcgacgatgc ggcggtgtac gatctgggca
    10741 atggcaccag cgttatcagt accaccgact tctttatgcc gatcgttgat aatcctttcg
    10801 attttggccg cattgcggcg actaacgcca tcagcgatat cttcgctatg gggggcaaac
    10861 cgattatggc gattgcgatc ctcggctggc cgattaacaa actttcccca gaaattgccc
    10921 gcgaagtaac cgaaggtgga cgctatgcat gccgtcaggc gggtattgcg ctggctggcg
    10981 gtcactccat cgatgcgccg gagccgattt ttggtctggc ggtgacgggg atcgtaccga
    11041 ccgagcgggt gaagaaaaac agtaccgcac aagccggatg taaactgttc ctgacgaaac
    11101 cgctggggat cggcgttctt accacggctg agaaaaaatc actgttgaaa ccagaacatc
    11161 agggactggc gacggaagtg atgtgccgga tgaacatcgc aggtgcgtcc tttgccaaca
    11221 tcgaaggcgt aaaagcgatg accgacgtta ctggttttgg tctgctgggc catttgagcg
    11281 aaatgtgtca gggcgcaggt gtgcaggcgc gcgttgacta tgacgcgatc ccaaaattgc
    11341 cgggggttga agagtacatt aagttgggcg cagtgcctgg cggcaccgaa cgtaactttg
    11401 ccagctacgg tcatctgatg ggtgaaatgc cgcgtgaagt gcgcgatttg ctgtgcgatc
    11461 cgcaaacttc tggcggtttg ctgctggcgg tcatgccgga agcagaaaat gaggttaaag
    11521 ctacagccgc cgagtttggc attgaactga cggcgattgg cgaactggtg ccagcgcgcg
    11581 gcggtcgtgc catggttgag atccgttaat tcaatgcggt tgtttattgc cgaaaaaccg
    11641 agtctggcgc gcgccattgc tgatgtcctg cccaaaccgc accggaaagg cgatggcttt
    11701 atcgagtgcg gtaatggtca ggtggtgacc tggtgtatcg gtcacctgct tgagcaggcg
    11761 cagccagacg cctacgacag ccgctatgcg cgctggaatc ttgcggattt gccgattgtc
    11821 ccggaaaagt ggcaattaca gccccgaccc tccgtgacca aacaacttaa cgtcatcaaa
    11881 cggtacctgc atgaagccag cgaaatcgtt cacgccgggg acccggatcg cgaagggcaa
    11941 ctgctggtgg atgaagtgct ggactatcta caactggcac cggaaaagcg ccagcaggta
    12001 cagcgttgct tgataaacga cctgaacccg caggcggttg agcgggcgat cgaccgtctt
    12061 cgttccaaca gtgagtttgt accgctgtgc gtttctgcgc tggcgcgagc gcgtgccgac
    12121 tggctgtacg gcatcaatat gacccgtgcg tataccattc tcggtcgcaa tgccggttat
    12181 cagggcgtac tttccgtggg acgcgtgcag acgcccgtgc ttgggctggt ggtgcgccgc
    12241 gatgaagaga ttgaaaactt cgtggcgaaa gacttctttg aagtcaaagc gcatatcgtg
    12301 acacctgccg atgagcggtt taccgctatc tggcaaccga gcgaagcgtg tgaaccgtac
    12361 caggatgaag aagggcgctt gttacatcgt ccactggcgg agcatgtggt taaccgcatt
    12421 agtggtcaac cggctatagt cactagctat aacgataaac gggaatcaga atccgcgccg
    12481 ctgccttttt cgctttcggc gttgcagatt gaagcggcaa aacgttttgg tctgagtgcg
    12541 cagaacgtgc ttgatatctg ccagaagctg tacgaaacac acaagctaat cacttatccg
    12601 cgttccgatt gtcgctattt gccagaagaa cattttgccg gacgccacgc ggtgatgaat
    12661 gccatcagcg ttcatgcacc agatctgttg ccgcagccag tggtagatcc agatatacgc
    12721 aaccgctgtt gggatgacaa aaaggtcgat gcgcaccacg ccatcatccc gaccgcacgg
    12781 agttctgcga tcaacctgac ggagaacgaa gcgaaggtct ataacctgat tgcccgtcag
    12841 tacttgatgc agttcagccc ggatgcggtg ttccgcaagt gtgttatcga actggacatt
    12901 gccaaaggca aatttgtcgc taaagcgcgt tttcttgctg aagcaggctg gcgcacgctg
    12961 ttaggcagca aagagcgcga tgaagaaaac gacggcacgc cattgcctgt ggtggcgaaa
    13021 ggcgatgagt tgctgtgtga aaaaggtgaa gtggtagagc ggcaaaccca gccgcctcgc
    13081 cattttaccg atgcaacact gctttcggcg atgaccggga tcgcgcgctt tgtgcaggac
    13141 aaagatctga aaaagatcct tcgtgcgacc gatggtctgg ggacagaagc aacgcgtgcc
    13201 gggattattg aactgttgtt caagcgtggt ttcctcacta aaaaagggcg ctatatccac
    13261 tccaccgacg ccggaaaagc gttattccat tcgctgccag aaatggctac gcgaccggac
    13321 atgaccgcgc actgggaatc ggtgctgacg caaatcagcg aaaagcagtg tcgctatcag
    13381 gactttatgc agccgctggt ggggacgcta tatcagctta ttgatcaagc caaacgtact
    13441 ccggtgcggc agtttcgcgg cattgtggct ccgggcagtg gtggcagtgc tgataagaaa
    13501 aaggctgcac cgcgtaaacg tagtgcgaaa aaaagtccgc cagcagatga agccggaagc
    13561 ggggcgatag cgtaagcgag cgaatctttc gtgctattcg agtcatattc tgaaatatcc
    13621 agcggatcaa gaaaattcgt tggatattt
//
LOCUS       DAZKFU020000123        13642 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZKFU020000123 DAZKFU020000000
VERSION     DAZKFU020000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.124"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..197
                     /locus_tag="ACHOQQ_RS14470"
                     /old_locus_tag="pgaptmp_002894"
     CDS             <1..197
                     /locus_tag="ACHOQQ_RS14470"
                     /old_locus_tag="pgaptmp_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471114.1"
                     /translation="MDDQLDQLLVTREIQDEIKSKIQDKIDELSKNLFNTMTETIENN
                     FDDIFRQQSENMSNYYEFVD"
     gene            complement(375..890)
                     /gene="pgpA"
                     /locus_tag="ACHOQQ_RS14475"
                     /old_locus_tag="pgaptmp_002895"
     CDS             complement(375..890)
                     /gene="pgpA"
                     /locus_tag="ACHOQQ_RS14475"
                     /old_locus_tag="pgaptmp_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGU4471115.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(868..1845)
                     /gene="thiL"
                     /locus_tag="ACHOQQ_RS14480"
                     /old_locus_tag="pgaptmp_002896"
     CDS             complement(868..1845)
                     /gene="thiL"
                     /locus_tag="ACHOQQ_RS14480"
                     /old_locus_tag="pgaptmp_002896"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGU4471116.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1923..2342)
                     /gene="nusB"
                     /locus_tag="ACHOQQ_RS14485"
                     /old_locus_tag="pgaptmp_002897"
     CDS             complement(1923..2342)
                     /gene="nusB"
                     /locus_tag="ACHOQQ_RS14485"
                     /old_locus_tag="pgaptmp_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGU4471117.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2362..2832)
                     /gene="ribE"
                     /locus_tag="ACHOQQ_RS14490"
                     /old_locus_tag="pgaptmp_002898"
     CDS             complement(2362..2832)
                     /gene="ribE"
                     /locus_tag="ACHOQQ_RS14490"
                     /old_locus_tag="pgaptmp_002898"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGU4471118.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2921..4024)
                     /gene="ribD"
                     /locus_tag="ACHOQQ_RS14495"
                     /old_locus_tag="pgaptmp_002899"
     CDS             complement(2921..4024)
                     /gene="ribD"
                     /locus_tag="ACHOQQ_RS14495"
                     /old_locus_tag="pgaptmp_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGU4471119.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVPIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(4028..4477)
                     /gene="nrdR"
                     /locus_tag="ACHOQQ_RS14500"
                     /old_locus_tag="pgaptmp_002900"
     CDS             complement(4028..4477)
                     /gene="nrdR"
                     /locus_tag="ACHOQQ_RS14500"
                     /old_locus_tag="pgaptmp_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGU4471120.1"
                     /translation="MHCPFCFAVDTKVIDSRLVDEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4628..5167
                     /locus_tag="ACHOQQ_RS14505"
                     /old_locus_tag="pgaptmp_002901"
     CDS             4628..5167
                     /locus_tag="ACHOQQ_RS14505"
                     /old_locus_tag="pgaptmp_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4471121.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHNVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLDFVRIHDIQPVMH"
     gene            5466..6350
                     /gene="tsx"
                     /locus_tag="ACHOQQ_RS14510"
                     /old_locus_tag="pgaptmp_002902"
     CDS             5466..6350
                     /gene="tsx"
                     /locus_tag="ACHOQQ_RS14510"
                     /old_locus_tag="pgaptmp_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGU4471122.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6540..6887)
                     /gene="yajD"
                     /locus_tag="ACHOQQ_RS14515"
                     /old_locus_tag="pgaptmp_002903"
     CDS             complement(6540..6887)
                     /gene="yajD"
                     /locus_tag="ACHOQQ_RS14515"
                     /old_locus_tag="pgaptmp_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGU4471123.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(7016..7987)
                     /gene="secF"
                     /locus_tag="ACHOQQ_RS14520"
                     /old_locus_tag="pgaptmp_002904"
     CDS             complement(7016..7987)
                     /gene="secF"
                     /locus_tag="ACHOQQ_RS14520"
                     /old_locus_tag="pgaptmp_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGU4471124.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(7998..9845)
                     /gene="secD"
                     /locus_tag="ACHOQQ_RS14525"
                     /old_locus_tag="pgaptmp_002905"
     CDS             complement(7998..9845)
                     /gene="secD"
                     /locus_tag="ACHOQQ_RS14525"
                     /old_locus_tag="pgaptmp_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGU4471125.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(9873..10205)
                     /gene="yajC"
                     /locus_tag="ACHOQQ_RS14530"
                     /old_locus_tag="pgaptmp_002906"
     CDS             complement(9873..10205)
                     /gene="yajC"
                     /locus_tag="ACHOQQ_RS14530"
                     /old_locus_tag="pgaptmp_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGU4471126.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10228..11355)
                     /gene="tgt"
                     /locus_tag="ACHOQQ_RS14535"
                     /old_locus_tag="pgaptmp_002907"
     CDS             complement(10228..11355)
                     /gene="tgt"
                     /locus_tag="ACHOQQ_RS14535"
                     /old_locus_tag="pgaptmp_002907"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGU4471127.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11410..12480)
                     /gene="queA"
                     /locus_tag="ACHOQQ_RS14540"
                     /old_locus_tag="pgaptmp_002908"
     CDS             complement(11410..12480)
                     /gene="queA"
                     /locus_tag="ACHOQQ_RS14540"
                     /old_locus_tag="pgaptmp_002908"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGU4471128.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            12573..13154
                     /gene="acpH"
                     /locus_tag="ACHOQQ_RS14545"
                     /old_locus_tag="pgaptmp_002909"
     CDS             12573..13154
                     /gene="acpH"
                     /locus_tag="ACHOQQ_RS14545"
                     /old_locus_tag="pgaptmp_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGU4471129.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVITILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYDALETRFWQFYPRMMAQASRKAL"
     gene            complement(13175..>13642)
                     /locus_tag="ACHOQQ_RS14550"
                     /old_locus_tag="pgaptmp_002910"
     CDS             complement(13175..>13642)
                     /locus_tag="ACHOQQ_RS14550"
                     /old_locus_tag="pgaptmp_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471130.1"
                     /translation="QEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQCLYNVAR
                     DGSPRYAGLLQQIASKCAILQDFDQQLFWRTFSGGVFHRRISVNSEPEKTDVWRFSTQ
                     FDFPHINDNGELSRTVLMFEGVAESNFKDIANHSSECFSYRCYAAPLDANAKK"
BASE COUNT         3388 a         3660 c         3309 g         3285 t
ORIGIN      
        1 aaatggatga tcaattggac caacttttgg ttacccgtga aattcaagat gaaataaaat
       61 caaagattca agacaaaata gatgaacttt ctaaaaattt attcaacact atgaccgaaa
      121 ccattgagaa taattttgat gatatttttc gtcaacagtc agagaatatg agcaattatt
      181 atgagttcgt cgactgacga tatttcagtg tcattgaatt atgagtgaca aagatacacc
      241 ttgaaatatc gccgcatagt atattcggcg atgtcgggtt ttactttaac agagtgcccc
      301 aaaattgggg cactgttcat ttataaatca aaccgggtgg tctggaatcg cctacacctc
      361 ccggtcaatg gcatttaaag aatacccagc ggccagtgat gaccgataaa gtacaggatg
      421 cctgcggaaa tcaccccggc gacaatatca tcgatcatga tccccatgcc gccatgtaca
      481 ttgcgatcaa accagcggat cggccacggc ttccacatat ccagaatacg gaaaatcaca
      541 aacccggcgg cgacccactg ccagtcattg gtcggcagcg ccatcagggt gatccacatg
      601 ccaataaact catcccagac gatgctaccg tgatcgtgca cacccatgtc tttcgccgtt
      661 tgatgacaaa gatagacgcc gatacagatc cccaacatca ccaccagcga gtagagctgc
      721 cagggcaaaa aggtcatcag ataccagaac ggaatcgccg ccagcgatcc catcgtgcca
      781 ggaacgattg ggcttaatcc acttccgaat ccgacagcaa gcagatgcca cggattactc
      841 atcttcaggc gacttttcgc gacatcttta tggcgtggca aaatggtcat atcctttcca
      901 gtctaacgtg acaggttcgc cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat
      961 ttgcccgata caggtaaacg gtacgcccag atgaccgaga gccacatcca gcgcgccacg
     1021 gttcagttcc ggcaccgtga aacacaactc gtaatcttca ccgccagaga gcgcccagcg
     1081 cagcgcctgt tccggtacaa catggcgaga aagcgcatca gaaaacggca gcaatgccag
     1141 gtcaatacgt gcgccgcagt cgctggcttt cacgatatgc ccgagatcgg aaatcagacc
     1201 gtcagagaga tcgatagctg aatttgccag atcgcgcagt gcctgcccct gtaaaatacg
     1261 cggcgatgga cggagatgac gtttgatcaa gtagtccgca tctttagcat cggcaacctg
     1321 caaacggttt tgcaaaatcg ccagcccggc ggcgctatcg cccggtgtac cggtcacata
     1381 gatccagtca cccggtttcg ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc
     1441 gtggataccc aacgtcattg ataatggccc acgcgtggta tcgccgccaa tgagttgcat
     1501 atcgtaataa ttgagaagat caaacaaact gtcgctgaag gactcaagcc acgcttcgtc
     1561 tacgtccggt aaggttaatg ccagcgtcag ccaggccgga tcggccccca tcgctgccag
     1621 atcgcttagg ttcaccgcca gtgctttata agccagatca gcaggatcga tatcagggag
     1681 gaaatggttg cccgccacca gcgtatcagt gctgatcgcc agggtctgtt tctcggggat
     1741 attgagaaga gcgcaatcgt cgccgatgcc cagttcgaca tcaagacgag aacttcttac
     1801 acggtcaaaa taacgggcaa tcagggagaa ctcgccacat gccatacgtt atgcctcagc
     1861 aggtaaaaaa gaaaaggccg gggatcgcgg aacagtcttc cgtgaatcta ccggcctgga
     1921 tattactttt tattagggcg aatcacaggt gctgctttat cgagtacgcc gttgacgaac
     1981 ttatggctgt cttctgcgcc gaacgatttc gccagttcga tcgcttcgtt aatggccact
     2041 ttgtatggca catcgctacg tttagacagt tcgtacagcg caatgcgcag tactgctttt
     2101 tctacctgtc ccagttcttc cagcaggcgg gacaggtatg gcttcatcag tccgtcgagg
     2161 tatgcggtat tagtcgccac cccggccagc agctcacgga agtacaggac gtcaacgtct
     2221 tttacatcct gttcagccag gaactggtat tcaacatcag cgatgtcgtt ctgggacaac
     2281 tgccaggagt agagcgcctg gacggcacac tcacgagcgc ggcgacgagc agcaggtttc
     2341 acggatttcc ccttactaat ttcaggcctt gatggctttc aatacattaa tcatttcaag
     2401 cgcggtcagt gcagcttctg cacctttgtt gccagctttg gtgccagcac gttcgatcgc
     2461 ttgttcaatg ctttcagtgg tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc
     2521 aacatgcgcc aggccgttgc ttgcaccacc agcgacatat tcaaagtggg cagtgccacc
     2581 acgaataacc gtacccagcg caatcaccgc gtcgtattta ccggttttag ccagtgcacc
     2641 cgccgccagc ggcagctcat aggcaccagg cacccaaaca acggtaatgt tttcatcttt
     2701 tacctgaccg atacgtttca gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa
     2761 gttgttgaaa cgcgcgatgg tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc
     2821 aataatgttc attttcttcc ttcgggttcg agtatggccc cgcagggggg cggattttag
     2881 cataatattt cgtgcgctgc ttccctttcg agccgggaga tcatgcaccc actaaatgca
     2941 ggcaaacatc cgggcctaca tgacgtatct ctttgaattt aaattggggg gcgtcggcta
     3001 atttctcaag ccctggcagc gagcataatc cacgggcgtc gctgcctaat agtttaggtg
     3061 cgatatagac aatcagctca tcgactaaac ccgcctgcag caatgcgcca gcgagcgttg
     3121 gccccgcttc cacccagatg ctgttaatct gctgtttacc cagttgcatc atcagtacaa
     3181 ccagatccag atgaccttta tgctctggaa tcagcaaggt acgcaccgtt tccggccact
     3241 cacgagaatc ttcctgcgta cgcgcgaacc aggtttcgcc gggctgctgc acaatgtgat
     3301 gttccggcgt cacgcgattt tggctatcaa tcacaatagg taccggctgg cggagatttt
     3361 gttgcgggta gagcgcctga gtttgttcat ccagttcgga ccaacgcacc gttaaggcag
     3421 gatcatccgc cagcaccgtg gcgctgctgg ttaaaatggc atgactttgc gcgcgcagtc
     3481 gttgtacatc acgccgcgcc tggggcgaag tgatccactg gctttcgccg ctcgccatcg
     3541 ccgtgcgacc atcaagcgat gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca
     3601 tccgtttgag aaagccttta ttcaattgct cggcttcact catcatcagc ccgtggctga
     3661 cgtcaatgcc agcctgttgc agacggtaaa gtccacgtcc agcaacctgc gggttcgggt
     3721 cctgcatcgc agcgaccacg cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg
     3781 gcgtacgacc atgatggcta cagggttcga gtgtgacata ggcggtcgca cctttggctt
     3841 tttcacccgc catacgcaac gcgtgtactt cagcatgtgg ttcacccgct cgttggtggt
     3901 aaccttcacc gacaatttcg ccatctttga caatgacgca cccgacattc gggttggggt
     3961 gggtggtaaa acgtcctcgt tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct
     4021 gcacggctta gtcctccagg cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac
     4081 tgcggtagac agaggcaaaa cggatatagg cgactttatc gagctttttc aattgctcca
     4141 tcaccagatt gccaatcatc ttgctcggca cttcgcgctc accggtggcg cgcagctgcg
     4201 atttaatatg attgattgcc atttcgacgt catcggaact caccggacgt ttttccagcg
     4261 cccgcagcat tccgctacgt aatttttctt cattaaacgg ttcacgcacg tcgttgcttt
     4321 ttacaacacg cggcataacc agctccgcca cttcaaaggt ggtgaaacgt tcattacaca
     4381 ccagacactg ccgacggcgg cgtacggatg aaccctcgtc cacgagacga gagtcaatta
     4441 ccttagtgtc cacggcgaaa cagaatgggc aatgcatacg gcgtcctgac caggtggtta
     4501 acagaaatct attttaccct gaactgacga tacaacaaag gcgcaacgct tttgagataa
     4561 aggatcgatg ctttcgctgc ttttaccctc taccattagg ctaattgcga cgagaaaggg
     4621 acgacctatg aatactaacg tttttcgact gctcctgctg ggaagcctgt tcagccttag
     4681 cgcctgtgtg cagcaaagcg aagtgcgaca gatgaaacac aacgtcagca cgctgaacca
     4741 ggagatgacg caactcaatc aagaaacagt caaaatcact cagcaaaaca ggctgaatgc
     4801 aaaatccagc agtggggttt accttctgcc tggagcgaaa acaccggcaa gactggaaag
     4861 ccagatcggt actttacgta tgtcgctggt gaatattacg cctgatacag atggcaccac
     4921 cctgacgcta cgtattcagg gcgagtcgaa tgatcccttg cccgcattca gcggaaccgt
     4981 tgaatatggg caaattcagg gaacaataga caactttcag gaaatcaatg tgcagaatca
     5041 attgattaat gcccctgcca gcgtcctcgc ccccagcgat gttgatattc cgttacagtt
     5101 aaaggggatt tctgtggatc agttagactt tgtacgtatc cacgacattc aacctgttat
     5161 gcattaaacg tattgcgggg caattttgtg cgtcccgcaa catctttccc cgtcattttg
     5221 ttactccgct tacatcaccc agattgatag taaaagtttg caacaagggt gaaagtcagt
     5281 acaatccctg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact
     5341 gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc tgtatacgaa
     5401 atatttagaa acgcaatttg cgcctttttc actcccgcaa gggattttca aacagtggca
     5461 tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct tcgtctttta
     5521 ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg caccagagcg
     5581 ttaacgttgt cggaagctat cacacccgtt ttggaccgca gatccgcaac gatacctacc
     5641 ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat gcggatgcgc
     5701 cggtattctt cggcggtaac tccgatgcca aaggtatctg gaaccacggc tctccgctgt
     5761 ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac cttagcttcg
     5821 gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt cgtaataaag
     5881 atggtcgcca gagcacctgg tacatgggtc tgggtaccga tatcgacact ggcctgccga
     5941 tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatggc gcagcgaacg
     6001 aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt
     6061 ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc gatttagggg
     6121 atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct atcgcttcca
     6181 gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct cgttactggc
     6241 acgacggtgg tcagtggaac gacgatgcag aactgaactt cggcaacggc aacttcaacg
     6301 ttcgctctac cggctggggt ggttacctgg tagtaggtta caacttctga ttatgaaaat
     6361 gccgggattt attcccggga tttattcccg gcatttcaga ttgttaacaa cgtgcgcttt
     6421 gttcatgccg gatgcggcgt aaacgctttg tccggcccac aaaagcacgt aaatgcaata
     6481 tattgctgaa ataacgtagg cctgataagc gtagcgcatc aggcaatttt acgttttaat
     6541 cacttcttct tgttcatcat cgctttcagg tcagcgaatg ggttgtactt cgcttcaccg
     6601 acatctttct gcgcatcttc ccctgcgata acggtcgtac catactgatc cgcttcggta
     6661 tatttcgaat gctcatgatc gtggcaatag agacaaagca attcccagtt actgccatct
     6721 tccgggttat tggtatggtc gtgatcaatg tggtgaacgg taagttcacg caggttggaa
     6781 taaacaaact cgcgggaaca gcgaccgcag acccacggat agatttttaa tgctttttcg
     6841 cgatagccgc tttctaaccg cgcatagttt tttgggatga tagccatgaa aagttacctg
     6901 gatgaaatgt agggttacgc gcagtttccc aagatgcggg gattttcgca atctccatac
     6961 atcaaaaaaa atcccgatct tctgaccggg attttcaaca tcaacgggaa cttgattaag
     7021 gcagaattga cggctgatcc gccccttctt tttccacttt ctgctgcaac atgtgttcgc
     7081 gcttcatacc cagtttcaga gccaacgcgg atgccacata gatggaagat gcagtaccga
     7141 tggaaacacc aatcagcatg gtcagcgaga agccttccag taccgggcca ccgaagaggt
     7201 acagcatcag gataaccatc aaggtagtac cggatgtgat caaggtacga tgcagcgtct
     7261 gggtcaagga cacgttaaag atttcgtaag gcgtaccgcg acgtatcttg cggaagtttt
     7321 cacgaatacg gtccgatacc acgatactgt cgttaagcga gtaaccgata accgacatca
     7381 acgatgccac aatggtcagg tcaatctcga tatggaataa cgacaaaata cccagcgtaa
     7441 tgataacgtc gtgcgccagc gcaataacca cccctgccgc cagtcgccac tcaaagcgga
     7501 aacctacgta cacgaggata gacagcagcg ctgccatcaa cgccatcgca ccggtttgcg
     7561 caaggtctgc ccccacgctc ggaccgacga actcaatacg cttcactgct gcattctgat
     7621 tggtggattc gttaatcacc ttcagaacct ggctgcccag cacctgaccg ccggtttcgc
     7681 cttcagcagg cggcatacgg accatgatgt cgtggctgct accaaagttt tgcagcattg
     7741 gctcttcaaa accggctttt tgcaatgcat cacgcattac gtcaatttca gccggttttt
     7801 cgagcgtaat ttcgataacc gtaccaccgg tgaaatccag cccccagtta aagccgcgca
     7861 cgcccataat aacgatagca gcgattaaca gcagaccaga gatgccgaaa gcccagtagt
     7921 cccagcgcat aaagtcatag actttacggc cgtggtttag ttgttcaaca gtatattcct
     7981 gtgccacatc gcactcctca gattgacagc ttcttgacgc gcttgccgcc atatagcagg
     8041 tttacgatgg cacgcgtacc gacaatcgcg gtaaacatcg acgtcgccac accgataccg
     8101 gtagtaatcg cgaacccttt aattgccccg gtacccactg cgtacaggat gatgacttta
     8161 atcagcgtgg tgatgttcgc atcgaagata gaactgaatg cgccacgata accttcatca
     8221 attgcctgtt gaacagtacg tccgttgctc aactcttctt taatacgttc gttgatcagt
     8281 acgttcgcat cgaccgctac cgcaagggtt aagacgatac ccgcgatacc tggcatactc
     8341 agtgttgcgc ctggcaacag cgacataata ccgacgatca agatcaagtt ggcaatcaga
     8401 gcactggtcg caatcagacc aaacttctta tagaagatga tcatgaacag aatagaaacc
     8461 agcagaccgg caaggcaagc ctccagcccc tgctcaatgt tctgcatacc cagggtcggg
     8521 ccaattgtac gttcttcaac aatctgaatc ggcgcgatca acgcaccggc acgcagcagc
     8581 agtgacagct gacgggcttc gttcgggttg ttgatgccgg tgatacggaa gctgttacca
     8641 agacgagact ggatgttggc gatgttaatc acctcttcct gtttcaccag aaccgcacga
     8701 ccatttgcat ctttcttacc gctgtctttg tactccacaa acagggttgc catcggtttg
     8761 ccgatgttgt ccttggtgaa gttagacata atgttgccac cagcgctatc gagcgagatg
     8821 ttaacctgcg gctggttgta ttcgtcctgg ctggaagtgg agtcggtgat atggtcaccg
     8881 gtcagaatta cgcgtttgta cagcacaacc ggctgacctt cgcgggtctg tttcacttca
     8941 gagtcgcccg gtacgcgacc ggatgccgca gcggcctgat caacgttggt gtttaccaga
     9001 cggaattcca gcgttgccgt cgcacccaga atctctttcg cacgcgcagt gtcctgaata
     9061 cctggcagtt caacaacgat acggtcagca ccctgacgct gaaccaccgg ctccgccacg
     9121 ccaagttggt ttacacggtt acgcaggata ttgatgttct gctgcaccgc atattcacgc
     9181 gcttcactca gacgagcatc gctcataacc gcacgcagct ggttgctgcc ctggctacta
     9241 atcaccaggt ccggatggcg cttgctcaga tacgcaatgg cttcatcacg agctttagca
     9301 tcgcggaaag tgatgctcag gccgtagttg ttttctttac gaacggtggt atacgggatg
     9361 cctttttcgc gcaggtcgct gcgcaggcta tcgatatttt gttcctggag tttgccaagc
     9421 gcggtgtcca tatccacttc catcaggaag tgaacgccgc cacgcaggtc aaggccgagc
     9481 ttcatcggct cagcgtgaat agctgccagc cagcgcggcg ttgccggggc aaggttaagc
     9541 gccacgacgt atttgtcacc cataacgccc attaatgctt cacgagcgcg cagctgggtg
     9601 tcagtggagt cgaagcgcgc aagaatagcg ccctcttcca gtgccacaga cttagcagtt
     9661 attttttctt cttgtaacgt tttctggacc tggatcagcg tttgctcact ggcggcgact
     9721 ccgcgcgcac cagtgatctg aacagccgga tcctcaccaa acaggttggg aagcgcatac
     9781 agcagaccga tgacaatcac cacgatcagc atgacgtact tccacaaagg ataacggttt
     9841 aacacggcaa ttcccttagg gaaaaatttt aattacagcg ccttcatggt gcctttcggc
     9901 aggacggcag ctacgaagtc acgtttaata actacttcag tggtgtcatt cagcgcgata
     9961 gcaatgtagc cgttttccgc tactttggtt acgcgaccga ccaggccacc gttcgtcaga
    10021 acttcatcac ctttggcaat ggagtccatc agctttttgt gttctttggt gcgcttctgc
    10081 tgtggacgca ggatcatgaa atagaaaatc agaccgaata ccaccagcat caaaatcaaa
    10141 gacatcgggc taccttgcgc cggtgcaccc gttgccgcta ccgcatcaga aataaaaaag
    10201 ctcattaaat ttccctcatt attaatatta atcaacgttc aaaggtggta cttctcgccc
    10261 ctgacgctgg taaaaatcag ttacgaagct ctctaattta ccctcttcaa tagccttgcg
    10321 taaacccgcc atcaaacgct ggtagtaacg aaggttatga atggtattga gtcgcgcgcc
    10381 taatatttcg ttgcaacggt caagatgatg caagtaagcg cgtgaataat tgcgacaggt
    10441 gtagcaatca cactcaggat cgagtgggcc agtatcgctc ttatacttcg cattgcggat
    10501 tttcaccacg ccatcggtca cgaacaaatg accatttcgg gcgttgcggg ttggcattac
    10561 gcagtcaaac atatcgatac cacgacgtac gccttcaacc aggtcttctg gtttaccaac
    10621 gcccatcagg taacgcggtt tgtctgccgg aatttgcggg catacatgct ccagaatgcg
    10681 gtgcatatct gctttcggct cacccacagc cagaccgccg acagcgtagc catcaaaacc
    10741 gatatctacc agacctttaa cagaaatatc acgtaaatct tcgtaaacgc tgccctgaat
    10801 gataccaaac agcgcatttt tgtttccgag actgtcaaaa cgctcacggc tacgcttcgc
    10861 ccaacgcaga gacatctcca tggagcgttt tgcgtaatcc cagtcagcag gatacggcgt
    10921 acactcatca aagatcatga cgatatccga accaagatcg tactgaatct ccatcgactt
    10981 ttccgggtcg aggaaaatcg ggtcgccgtt gatcgggtta cggaagtgca cgccctgttc
    11041 ggtgatttta cgaatatcgc caaggctgaa gacctggaag ccgccggagt cggtgaggat
    11101 cggtcccttc cactgcataa aatcgtgcag atcgccgtgc agtttcatga tttcctggcc
    11161 cgggcgcagc cacaggtgga aggtgttgcc gaggataatt tgcgcgccag tggcttcaac
    11221 ttcttctggc gtcatccctt ttacggtgcc gtaggtgcca acaggcataa aacaaggcgt
    11281 ttccactacg ccacgatcaa agaccaggcg gccacggcgt gcgcgaccgt cggtggtgtc
    11341 cagttcaaat ttcatttttt ctccactacg tcagaaaaac agtccaacgt ttaaaccagc
    11401 gccgcggaat tactccccga cgcgctcatt aattgcctgc ggattgtacg tgataaacat
    11461 cgcatcaccg taactaaaaa agcgatattt ctcttctacc gccgctttat aggcattcat
    11521 ggtgtgttga taaccggcaa aggccgaaac cagcataatc agcgtcgact ctggcaagtg
    11581 gaagtttgtc accagcgcat cgaccacttt gtactggaag cccggataga taaagatttg
    11641 ggtatcgtca aagaacggtt cgatgagatc gtttttcgcc gcctgagccg cgctttccag
    11701 cgaacgtact gaggtagtgc caaccgcaat cacccggtta ccgcgcgctt tcgccgccag
    11761 taccgcgtct accacatcct gcggtacttc agcgtattcc gagtgcatga tgtgatcttc
    11821 aatggtgtcg acgcgcaccg gctggaaggt gcccgcacca acgtgcaggg tgacgaacgc
    11881 catctccacg cctttggcgc gtaatttttc cagcaaaggt tcgtcaaaat gcagaccagc
    11941 ggtcggggct gcaaccgcgc ccggtttttc gctataaacg gtttgataaa gttcgcggtc
    12001 agcgtcttcg tccggacggt cgatatacgg cggcagcggc atatggccga tgctgttgag
    12061 aatatccagt accgagcgtt catcattaaa ttcgacttca aacagtgcgc cgtggcgcgc
    12121 ggtcattgtt gcgttgatac tttcgtcatc gcccagcaac agttctgcgc caggttttgg
    12181 cgctttcgag gcgcgaatat gcgcaagaat gcgtttgtcg tcgagcatcc gctcaaccag
    12241 cacttcaatc ttgccgccgc tggctttacg cccaaacagg cgcgccggga tcacgcgggt
    12301 attattaaaa accagaagat cgccggggtt gagcttatcg agtaaatcgg tgaaagtacc
    12361 gtgcgtcagc gcgcccgtcg gcccgtccag cgacagtaaa cgacagctac tgcgttcagg
    12421 catcggatag tgggcaatca gggattcggg caattcaaag gagaaatcgg taacgcgcat
    12481 gacactgact cagactaaaa taagaggcgg gtagtctagt gccggggcgc tctccctgca
    12541 acctttaccc ccctttggat agaatataat cgatgaattt tttagctcac ctgcatttag
    12601 cccatctcgc ggaaagctcg ctttccggca atttgctggc tgatttcgta cgcggaaatc
    12661 ctgaagaaag ttttccgccc gacgtcgtgg ctggcattca tatgcatcga cgtatcgacg
    12721 tattgactga caatctgccg gaagtccgcg aagcacggga gtggtttcgt agtgaaactc
    12781 gccgcgttgc gcctattacg ctggatgtca tgtgggatca ctttctttcc cgtcactggt
    12841 cgcagctgtc gccggacttt ccgctacagg aatttgtctg ttatgcccgc gaacaagtga
    12901 taacgatttt gccggactca ccgccacgct ttatcaacct gaacaactac ttatggtcag
    12961 agcagtggct ggtgcgctat cgcgatatgg atttcatcca gaacgtgtta aatggcatgg
    13021 caagccgtcg cccacgtctg gatgccctgc gtgactcctg gtacgattta aacgcccatt
    13081 atgacgccct cgaaacccgc ttctggcagt tttatccgcg gatgatggcg caggcgtcac
    13141 gcaaggcgct ctgaaacgtc aaactccggc gtcactactt tttagcattc gcatccagcg
    13201 gggcggcata acaacggtaa gaaaaacatt cgctggaatg attggcgata tctttaaagt
    13261 tgctttcagc aaccccctcg aacatgagta ccgttctgga caactctccg ttgtcattta
    13321 tatgtggaaa atcaaattgt gtactgaatc gccatacatc tgtcttttct ggctcgctgt
    13381 tcacagagat acgtcgatgg aagactcctc ctgaaaacgt gcgccagaac aattgttgat
    13441 caaaatcttg taatatcgca cacttggatg ctatttgttg tagtaaacct gcataccgtg
    13501 gactgccatc acgagcaaca ttatacaaac actgactaaa agcatcgaac aaacaataga
    13561 tgtgatcact agtgaaaaaa ggtcttaatt ttttttgttc ttcgtcgctg taatccagga
    13621 gcatctcatt gatttcttcc tg
//
LOCUS       DAZKFU020000124        13404 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZKFU020000124 DAZKFU020000000
VERSION     DAZKFU020000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.125"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..814
                     /locus_tag="ACHOQQ_RS14555"
                     /old_locus_tag="pgaptmp_002911"
     CDS             <1..814
                     /locus_tag="ACHOQQ_RS14555"
                     /old_locus_tag="pgaptmp_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU4471131.1"
                     /translation="NTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIYLLPLAR
                     GNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESEGITTTGRMLYRNLKITSALDE
                     IYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGTTIGY
                     ISTLPVSQAKRWTNEQPRIDIYIDQIITVSGIANSSGFALAALLNANIEMGNDPIIGI
                     EAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(955..1728)
                     /gene="uxuR"
                     /locus_tag="ACHOQQ_RS14560"
                     /old_locus_tag="pgaptmp_002912"
     CDS             complement(955..1728)
                     /gene="uxuR"
                     /locus_tag="ACHOQQ_RS14560"
                     /old_locus_tag="pgaptmp_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGU4471132.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1943..3403)
                     /locus_tag="ACHOQQ_RS14565"
                     /old_locus_tag="pgaptmp_002913"
     CDS             complement(1943..3403)
                     /locus_tag="ACHOQQ_RS14565"
                     /old_locus_tag="pgaptmp_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGU4471133.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3484..4668)
                     /gene="uxuA"
                     /locus_tag="ACHOQQ_RS14570"
                     /old_locus_tag="pgaptmp_002914"
     CDS             complement(3484..4668)
                     /gene="uxuA"
                     /locus_tag="ACHOQQ_RS14570"
                     /old_locus_tag="pgaptmp_002914"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGU4471134.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5008..6351
                     /gene="gntP"
                     /locus_tag="ACHOQQ_RS14575"
                     /old_locus_tag="pgaptmp_002915"
     CDS             5008..6351
                     /gene="gntP"
                     /locus_tag="ACHOQQ_RS14575"
                     /old_locus_tag="pgaptmp_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HGU4471135.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLIGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6525..7427)
                     /gene="fimH"
                     /locus_tag="ACHOQQ_RS14580"
                     /old_locus_tag="pgaptmp_002916"
     CDS             complement(6525..7427)
                     /gene="fimH"
                     /locus_tag="ACHOQQ_RS14580"
                     /old_locus_tag="pgaptmp_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4471136.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPNYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7447..7950)
                     /gene="fimG"
                     /locus_tag="ACHOQQ_RS14585"
                     /old_locus_tag="pgaptmp_002917"
     CDS             complement(7447..7950)
                     /gene="fimG"
                     /locus_tag="ACHOQQ_RS14585"
                     /old_locus_tag="pgaptmp_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HGU4471137.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7963..8493)
                     /gene="fimF"
                     /locus_tag="ACHOQQ_RS14590"
                     /old_locus_tag="pgaptmp_002918"
     CDS             complement(7963..8493)
                     /gene="fimF"
                     /locus_tag="ACHOQQ_RS14590"
                     /old_locus_tag="pgaptmp_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGU4471138.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8503..11139)
                     /gene="fimD"
                     /locus_tag="ACHOQQ_RS14595"
                     /old_locus_tag="pgaptmp_002919"
     CDS             complement(8503..11139)
                     /gene="fimD"
                     /locus_tag="ACHOQQ_RS14595"
                     /old_locus_tag="pgaptmp_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGU4471139.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVVCAFAAQTPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTSWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLSQLSAECR"
     gene            complement(11206..11931)
                     /gene="fimC"
                     /locus_tag="ACHOQQ_RS14600"
                     /old_locus_tag="pgaptmp_002920"
     CDS             complement(11206..11931)
                     /gene="fimC"
                     /locus_tag="ACHOQQ_RS14600"
                     /old_locus_tag="pgaptmp_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4471140.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11968..12507)
                     /locus_tag="ACHOQQ_RS14605"
                     /old_locus_tag="pgaptmp_002921"
     CDS             complement(11968..12507)
                     /locus_tag="ACHOQQ_RS14605"
                     /old_locus_tag="pgaptmp_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4471141.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12572..13126)
                     /gene="fimA"
                     /locus_tag="ACHOQQ_RS14610"
                     /old_locus_tag="pgaptmp_002922"
     CDS             complement(12572..13126)
                     /gene="fimA"
                     /locus_tag="ACHOQQ_RS14610"
                     /old_locus_tag="pgaptmp_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4471142.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLKQTGATSSAVGFNIQLNDCDTSVATKAAVAFLGTA
                     IDSAHPKVLALQSSAAGSATNVGVQILDRTGNELTLDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         3249 a         3467 c         3232 g         3456 t
ORIGIN      
        1 caataccctt cagagccaaa ttattacaga taatcacttc cttcatcatc caaaagtcga
       61 cagtgagtta acacgtaaat atgaacgtgc acgcctggat acggaaaata tctatcttct
      121 gcctcttgct cgtggcaata accataatta cgatggaaaa agcgttgtcg aaattagaaa
      181 acttgatatt agtaaagagt cctggccatt taattatgtg acagaagcat gcagagaatc
      241 cgaaggtatt acaacaacag gacgtatgct ctatcgaaac ttgaaaatca cctcagcttt
      301 agacgaaata tatggtggca tatgtaaaaa ggcgcatgct gctacagaat tagcagaagg
      361 tttacggcta aatttattta tgaagagccc tttcgatcct gttgaggact atacagttca
      421 tgaaataaca ttaggccctg gttgcaatgt tccaggttac gccggaacca cgataggata
      481 tatttcaacc cttccagttt ctcaggcaaa aagatggacc aatgagcagc caagaattga
      541 tatctatatt gatcagataa taactgtctc agggatagca aattcttccg gattcgccct
      601 tgccgcatta cttaatgcta atattgagat gggaaatgac ccaatcattg gtatagaagc
      661 ctatcctggt accgcggaga tccatgcaaa aatgggatat aacgttatcc ccggtgatga
      721 agatgcgcca ctcaaacgca tgactctgca accgtcatct cttccagaac tgtttgaatt
      781 aaaaaatggt gaatggaatt atattggtaa atagccactg agaagaatga gtactaatat
      841 tcaggttata cagatattta aacaacaccc tgcaatttac aaagtaatac tcattgcgac
      901 ttcttaattt aagaaagcgc aaggaacgtt tacccttgcg cttattataa taagtcaggc
      961 gtcgacttta tccagcggcc atgaatcaaa cagatagccg tcaaaataaa tatcgtcaac
     1021 gttcgagaat tccagcagac gttgcttaac gttttccaga tgctgccaca ttgccagctt
     1081 cgccgctcgg gcatcttttt tgattaacgc ggcgaggatc tgtttgtgat cgcccaacca
     1141 ctctttgcga tacaggctgt catccagatg gctgtgcaac tggatccaca ttggattgtt
     1201 ttcccgccac tgccaggact gacggaataa ctccaccagc atgctgttat gcgttgcttc
     1261 ggcaatggcg agatggaact gcatatcacc gctttcgctg ctacccggcg cgctggaagc
     1321 cagttcacgc tcttccagtt gcaatgcctg acgcattttg acgatatctt cgcgggtagc
     1381 ctgcagggcg gcaaactcgg cgatgttact ttccagtaac tgacgcgcct gtaacagctc
     1441 aaaaggaccc gcatcattgc agacgttggc atccggactg tctgtgttgt ggctgcctga
     1501 gctatcaaga acatagatac cggcaccccg gcgcacttcc accagccctt tgatctccag
     1561 catgatcagc gcttcacgta ccaccgtccg cgtgacatca agcatttctg caatttcacg
     1621 ctccggcggt agccgttcac caggattgta cggcgtcttt atgatcagat cgcggatcat
     1681 cgccccgact tcctggtaag gtctttgcgc agaggtggca gatttcatgg tgttgtgtcc
     1741 gttaggtgcg gttaatcaaa tttttgccgg atgcggcgtg aacgccttat ccggcctaca
     1801 acgggcaatt ttacggcccc cgttaatctc aaactatacc attagcactg gaagttggca
     1861 cgtacaggtg ggcaaaaaaa cgaaaactgg tcaaccaaat atcagtttga ttgaccagta
     1921 agtctgtagg gtaatcagtt agttacagcg cagccacaca ctcgcgcgca ccgcgttcgc
     1981 acagctgctg atatgccgcc gtcaccgcgc caacaaagtc ggcattctgc ggcagatcat
     2041 cggcaaaaat accgctcagg cccagcagcg ctttcacgcg gtctgcgccc tgatactgcg
     2101 cgttaatctt ctggaactcg gccagcatcg ggtcgaccac gtcaatggca ttaccctgct
     2161 catccacgcc ctgggtgtaa cgcatccagc cagccacgcc cagcgccagg tgacgccagc
     2221 tgccgccgtt ttgcaggtgc agacgcaccg gatccagcag acgctgcggt aacttctggc
     2281 tgccgtccat cgcaatctgc caggtacggt gacgcagaga cgggttgctg aaacgctcga
     2341 tcagcagcgt cgcataggcg ttcaggtccg taccttccgg catcgacagc gttggcgctt
     2401 gttcctgcat catcagggca aaggccgctt tgcgataatc cgggttagtc atggtgtcgg
     2461 caatggtttc atagccgccg aggtaaccga ggtacgccag gaaagagtgg ctgccgttca
     2521 gcatacgcag tttcatcatt tcgaacggca caacgtctgc aacgaactgt gcgcccactt
     2581 tatcccagtc cggacgacca ttaacgaagt tatcttcaat cacccactga cggaacggtt
     2641 cgcaggcaat ggcgcacggg tcgtaaacac ccagctggtc agcaatttcc tgtaaggttt
     2701 ctggcgtcgc cgccggaacg atgcggtcaa ccatggtgca cgggaaggtg acgttctctt
     2761 caatccatgc cgccagctgc gggtcacgcg cctgagccag ccccagaacc gcgaccttcg
     2821 ccacatgacc gttttcacgc acgttgtcgc aggacatcac cgtaaacgct ttcaaccctt
     2881 tctcacgacg cagacgcaac gcttcgacga tgtaaccaat cgcggacttc ggcgcagtcg
     2941 ggttttccag atcgtgcttg atcagtgggt tattgagatc cagttgaccg cttgccgcat
     3001 cagcgcagta gcctttttcc gtgaccgtta gagagacaat cgccgtttgc ggacgcgcca
     3061 tcgcgttgag aataccttcg cagccgtcga tttccggatg cagcgcttct ttcatcgaac
     3121 cgataatttt cagctcggtg ctttctgcgc ctttttccgc tacggtgtac agcagttgct
     3181 gttttttcag gttttcgatc agcacgcggt cgttgcctgg catcaggtta acttcgcaga
     3241 tgccccagtc gctgtcggtg ctttccagca gatgatgggt atacagcgcc tggtgcgcgc
     3301 ggtgaaacgc cccgcaaccg agatgcacaa tgcgtgattc cagacgagaa tgatcccatg
     3361 acgggcgggc aaccggcaga ttgctgtcaa caatagtagt catcgtaaac tccagaatcc
     3421 gtcttcagag agtgacggca aggaagaagg aaccggcacg gcgcgcagcc atgccggtgg
     3481 atattaacgg ctaaagaaag cgcgctggat cgccagttcg acaccgcgaa cttcggccag
     3541 gcctttcaga cgaccaattg cggagtaacc tgggttggtt ttcttcttca ggtcgtccag
     3601 catctgatga ccgtggtccg gacgcatcgg gatcaggtct tctttgcctt ccgctttacg
     3661 acggtgttct tcttcaacaa tcgctttcac cacttcgtac atatcaacgt caccgttcag
     3721 gtgcgccgct tcgtggaagg ttttcgggtt atcttcacgc atggtggagc gcagatgggt
     3781 gaagtaaata cgcggaccga actgcttgat catatcaacc agatcgttgt cagcacgcac
     3841 gccgtaggaa ccggtgcaca tggtgaaacc gttcgccatg ctgtttacgg tatcaaccat
     3901 ccactgcata tcttcaatgg tggaaacaat gcgcggcagg ccgaggatcg ggcgcggcgg
     3961 atcgtccggg tgaaccgcca tacgcacgcc aacttcttca gcaactggaa taatcgcttt
     4021 caggaagacg gcaaagtttt cgcgcagctt cgctttatcg atatctttgt acagctccag
     4081 gtgcttacgg aactggtcga gggtataccc ttcttccgcg cccggcagac cggcaatgat
     4141 gttacgcgtc agacgcgctt tgtcttcgtc gctcatagtg gcgaagcgtt cagctgcctg
     4201 agcaatttct tcttcggtgt aatccgcttc cgcgcctgga cgtttcagga tatgcatttc
     4261 gaatgcagcg aattcgatct ggtcgaagcg cagagctttg gagccgtctg gcagcacgta
     4321 ttcgaggtca gtacgggtcc agtcgagcac cggcatgaag ttgtagcaca cggtgcgaat
     4381 accgcactgc gccaggttgc gcagagtctg ctgatagtta gcgatccact gctcatagtt
     4441 gccagtgtgg gttttgatat cttcgtgaat tggtacgctt tctacgacag accacaccag
     4501 gcctgcgtct tcaacgatcg ccttgcgttt gaggatctct tctacggacc atacttcgcc
     4561 gttcgggata tggtgcagcg cggtaaccac gccagttgcg cccgcctgac ggacatcagc
     4621 taaagaaacc ggatcgtttg ggccgtacca gcgccaggtc tgttccatga tttttcctct
     4681 ttcacatagt gggttccaca gcaaagagac gcggaaccct ggagttgttt aggtatccgg
     4741 atgaacaacc agaccgcagc cgtggcgttg catcggatga gatggcgtat aagttctacc
     4801 ttttggtaag acatgtcaac ccgaattcta aaattggtta accacatcac aagaatttca
     4861 ctctcatggt tgttgctgca aattgcagca aagggtaatt gatggttatt gatcagagga
     4921 tgtgacattc atcgcaacaa tggttgacca atttacataa catatcgccc aaaataacaa
     4981 cggttcaacc aggtaaggta aaacgttatg catgtgctta acattctctg ggtggtattc
     5041 ggcattggtc tgatgctggt actgaatttg aagttcaaaa tcaattcaat ggtggctttg
     5101 ttggtggcgg cgctgtccgt cgggatgctg gcgggcatgg atttgatgtc gctgctgcac
     5161 accatgaaag cgggcttcgg caacacgctg ggggaactgg ctatcatcgt ggtgttcggt
     5221 gcggtcatcg gtaaattgat ggtcgactcc ggcgcggctc accagatagc gcatacgctg
     5281 ctggcgcgtc tcggtctgcg ctatgtacag ctgtcagtga ttatcatcgg cctgattttt
     5341 ggtctggcga tgttctatga agtggccttt atcatgttag cgccgctggt tattgttatt
     5401 gccgccgaag ctaaaattcc gttcctgaaa ctggcgatcc cggcagtagc agctgccact
     5461 accgcacatt cactgttccc accgcagccg ggtccggtgg cgctggtgaa tgcttatggc
     5521 gcagatatgg ggatggttta tatctatggc gtactggtga cgatcccaag tgtaatctgc
     5581 gcaggtctga tcctgccgaa gttcctcggc aatcttgagc gcccaacgcc atcattcctg
     5641 aaagcagatc aaccggtaga tatgaacaat ctgccctctt tcggcgtttc gattctggtg
     5701 ccgctgatcc cggcgatcat tatgatctct accaccatcg ccaatatctg gctggtaaaa
     5761 gatacccctg cctgggaagt ggttaacttt atcggttcct cgccgattgc aatgtttatt
     5821 gcgatggtgg ttgcattcgt actctttggc accgcacgtg gtcatgacat gcagtgggtg
     5881 atgaacgctt ttgaaagtgc ggtgaaaagc attgcaatgg tgattctgat catcggtgcg
     5941 ggtggcgtgc tgaagcaaac tatcatcgac accggcattg gcgacaccat cggcatgttg
     6001 atgtcccacg gcaatatctc gccctacatt atggcatggc tgatcactgt gctaattcgt
     6061 ctggcgacgg gtcagggtgt cgtttcggcg atgaccgccg ccgggattat cagtgctgca
     6121 atccttgatc cagcaactgg tcagctgatt ggcgtgaatc cggcgctgct ggtgctggcg
     6181 acggctgcgg gttccaacac cctcacccac attaatgatg cctcattctg gctgttcaaa
     6241 ggttactttg acctgtcggt aaaagacacg ttgaaaacct ggggactgct ggagctggtc
     6301 aactccgtgg ttgggctgat tattgtgttg attattagca tggtagcgta aaagaacacc
     6361 agagcctgcc aatggcaggc tcagttcgga ttgtcggcaa agtgcgcttt gtttatgtca
     6421 gatgcgacgc tgacgcgtct tatctggcct acaaagggct aacgtgcagg tttttagctt
     6481 caggtaatat tgcgtacctg cattagcaat gccctgtgat ttctttattg ataaacaaaa
     6541 gtcacgccaa taatcgattg cacattccct gcagtcacct gccctccggt acgtgcgtaa
     6601 tttgccgtta atcccagact taccgccgaa gttcctactg ctcctaacga taccgtgtta
     6661 ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc cgacgccctg cgctggtgaa
     6721 aacgacgcgg tattggtgaa aatcgagttg cccgcatctg cggttgtgcc ggagaggtaa
     6781 taccccaggt tttggctttt cgcacaataa acggtaagag gaattggcac tgaaccaggg
     6841 tagttcggca gagtaacggt gacatcacga gcagaaacat cgcagccgcc agtaggcacc
     6901 accacatcat tattggcgta aatattccac acaaactgga aatcatcgct gttatagttg
     6961 ttggtctgtc gcaaaataag cacggcaatt aatgagccag ctttaatcgc caccccgccc
     7021 gcactgctca caggcgtcaa ataaagcgcc accggccacg gcttatccgt tctcgaatta
     7081 taaacaaccc gcggcgtttc gctggtggtc ggaaatggat agctactgcc actatatttt
     7141 acggtcccgg aaaaattaga taacacgccg ccataagccg agcctcgttg cagtgtgaca
     7201 tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga tttgcgtcga aagatctacg
     7261 accaggtttt gccccacatt cacggcaggc gcaaggttta cataaacatt agcgctgcca
     7321 ccaccaatag ggatagcggt accattggcg gttttacagg cgaatgacca ggcatttacc
     7381 gaccagccca tcagcagtac agcaaacagg gtaataactc gtttcattac aatcatctct
     7441 tcgggttcag ctgtaggtat aggtgatgct aatcactgcc tgaatggttc cctgagtggc
     7501 tccgccattt accgttaatg ctctgacctg taacgggaag tgcgctgatt gtgaggaatc
     7561 atccacctga actgttttgg ttgcgccagt attcaatgtg ttgccactgt catcctgtag
     7621 ctctaactgg atgttttgcg cggtcccctg gtttttataa tatccggtac tgtcggctgc
     7681 cccgctgaag ctggcagtga ccctcgacgt tcccaccgga caattagtca actcaagcgc
     7741 aacatcatgc caggccgatg ccgccccggc agacatcaga ctgaaagaat aaagatcgcc
     7801 gagatcaacc gtggcattgg tggtggaaac tgtgcacggt ttggcgacga ccttaccgtt
     7861 caccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt gcgagcgcca atattgccgc
     7921 caataaatac ccacgtttgc gccatttcat gagcatctcc agttactgat attcaagagt
     7981 gaaggtagcc gtggcattga tatgccccgc agtgacaggc acctgtgtcg ccattagccg
     8041 ggcgtaaaaa ttcaacgtat ttggtttacc cggcgtcagg gtcgtccacg aaagcgcgga
     8101 cgatggagca ttaaggggta tttgattttg ctgctcattc agaagctgta ttcccagtcc
     8161 cgaagccgct gacaccgtat tttcaagtgc aagcaggttg gcattgtggc tatctgcaac
     8221 accggtaaac ccaactttta cggcagaaac ggcattacca cagggtgaca gcaaaatacg
     8281 aaatggaacg acaggagtcg tcgcgccaat gttgttaaat tgcttcgccg cgttttccat
     8341 caaatcaaca gtaaaattgg ttgattcagc ggccacacta cagccgttat ctctgacata
     8401 gccgcggata gtaatcgtgc tatccgcagc caaagcgtga ctcaccgcca gccacaaaaa
     8461 agcgcacaga agataaaaag gtttgtttct catcacgccc ccttaacgac attcagctga
     8521 tagctggctt aataactgct gctgactctc tggtggcagt tgataattgg cgacacagtg
     8581 agcattttcc tcttctcccc atttcacctg aacttttccc gctaaaggca ttccgctgag
     8641 gtaaacctga ccattatccg caacaatgcc gctactctgg ctactctctg atgtcaccat
     8701 cgccccaaac ggcagcggct tattattgtg ggttagcgtc atgagcagtt ttatcccaac
     8761 gcgcgcttta aactctgctc gcacgatcgc cccacgagtg ggaacaacgt tagcgaccgc
     8821 gttatctaaa tcgacgttat cagccagggt attggtatcc agcgccactc tgttttcccg
     8881 atattcggtg gcataaggca gcacggcata accgcgccag tcggtacgca cccccgtctg
     8941 gttttcgact tttgcatctt ttgcgccagg cgctttaaca agcaccaccg tatcgtttaa
     9001 cggctgcccc agcgttacgc cattggcatg agccagtacc ccaccgctga ctccgtaata
     9061 gagctgctta atatcatcgc tatggctgta accgatattg gcattgccgt aaccaccgcg
     9121 ataattcagc gtggcgtagc ctgtgcttcc gctattacca tcgcctcccc cggcatagcc
     9181 ggtttgcacg ctatagctga ggttgttgtc ttccagcaac gtaccgtata caccagccag
     9241 attggtcatc cgaccgttga gatcgtgtga catgctgtag ctggcactgg catgtcgcca
     9301 ctgagatttg ctgtcagaac gcagccagtg gctgaaagga atattgacgt taagcgctaa
     9361 catctgatcc cgtccttttt gccaggcgtt tttcgtcagg ctatagctga gcgtccagtt
     9421 gatatcttcg aacgcagtat ttaatccagc ctggaattgc tcatcgacat tactcgttcc
     9481 ccaataagtt tgatggctac cactcaaata cagtgttgat gtgcgcccga gttgctgagt
     9541 aacggtgagc tgtaatttcc cgcgtttgtt ataagcgagg ttgtaatagt cggtgaattt
     9601 cggcttaacc tgaataactc catcctgtgt ttcgatgttg tagccattca ttcgactgta
     9661 tgttgtatca gcgaaattaa aatatccgct ggtcgaataa cggtaaccca ctaactgaat
     9721 attcgtgcct gactcattga gcgatttgtt atagagaaaa cgcaccgatt gtccgtcatg
     9781 ctgactgtca tcgggaagtg tggaattagc ctgcgtcata tccacagaca gagcgcccag
     9841 tgcccccatg tttttcccga taccgaaatt aaaagcacga taacgatccg ccagttgcgt
     9901 tccaccatat attgtccagc cagccggaag gccgtggagt aatgtactct ggaaaaagcg
     9961 gggtttttcc tgctgcgcat ttccactacg gtattctcct gccgtaatgg aataacgagt
    10021 atgcccttca cgttgcaaaa gcgggactga cgaatagggt acggtaaaaa tctgcgtgct
    10081 gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta ttacctgcgg catagatatc
    10141 gttgatggta aaaggccccg gtggcaccgt actattataa atgtcatacc cattttgttt
    10201 aatagtgacc tgtgcagtac cacgagcaat accgtggatc accggggcaa atcctctttg
    10261 gctatcgggt aacatattgt catctgaggc caattgtgcg ccgcgaaagt taataccatc
    10321 gaaaatatcg ccctgggtat aaccatcacc cagcgtcagc cgggaacgta acggtattat
    10381 gtctcgctca agccaggtat tgatatgctg ccatttattt ttgctacctg atgaactgtc
    10441 gctactgtta taactccagc tggtattgtc gcgtaaacgc cacgcaccaa tatttaaccc
    10501 actctgtagg tttaaatatg cataatggct gttaccccca atccgattct gtacactatt
    10561 tccgctgaaa ttataattga gcaatccggc attaataccg ggatcccata actcaggagg
    10621 aatataacca cgcgcgcgat tactcataaa tgcctgaggg atcgtcaggt tcagtcgctg
    10681 ctgaccaaca tctaaatgcg cagtagcgtc ctggaccatt gtggttaatg gcacgcaggc
    10741 atcatccgcc agcagattca taccggagac agaagccgta ttcagcccca tactggcaag
    10801 ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcgctg tcgcccgtat taaatgtgac
    10861 atcacgcgtt gccatataac cattattcaa atagatatcg acacgatacg tccctggcgg
    10921 taattcttgc ccattttcaa aacgcgataa atcagccaca gcctggggat catccgctaa
    10981 aaagcgcgga ttaaaataga gttcggcaga tgacaaaggt gtctgtgcgg caaaagcaca
    11041 gacgacaacg agccggacaa aaaaaccagc caaacgatgc ttacgaatat gcaagcattg
    11101 tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt attcctccgg tttaaaatca
    11161 cccggcagtc aattcttttt attcaggcga aaaattcccc ctgcgttatt ccattacgcc
    11221 cgtcattttt ggggtaagtg caccataatc atttattgtt cggtaagtaa tattgcttcc
    11281 tgcatcagaa ggcaatttaa ccgtgctttc gcccattgga ggcaccaatg cattttcaag
    11341 aacccgggtt ccggcattca actctgttac cgtcaggtaa tagggtgtcg ggttaatcag
    11401 cgtcagagaa ttcgcgctac gacgaaatct taatttttct gcggcctgat cgggtggcaa
    11461 cgctaattta gccggacgat agtacagttt aatgcggctg ataattgcga gctgtagcgt
    11521 attctcagtc aattttgatt tatccattga cggaatcgct ttaacgttca tccagaataa
    11581 actttcccgg tcctgtggca attggttatt tgttgcatca agaatacgca aggtattctc
    11641 tttttttccc ttcatcgcaa acagaggagg cgtcacgata aaacgaccat cctttacacc
    11701 atcggcattt tccacccatg attgaattaa ataggtacta ttttcatcat tatttgtcac
    11761 ggcaagttgc acttgttttt gccctgccgg ataaattacg cgagtcgcac ctaaggccac
    11821 tcccgcttca gcgcgtccgg caaccatcat tgccatgaac atcaggatac ctgccagcaa
    11881 gcagaatgtt atttcctgcg atttccttac attgacgttt ttattactca ctgtcctgtt
    11941 cctgttatca cattatctgc tgaacaatta ctgataggtt aaagagaacc aggcctgtgc
    12001 attggcgatg ccgccagtaa cccgacgccc ggtagcacga tatttggcga tgaaatgtag
    12061 cgaagtagag cctgagtaaa gccgtttcca gtttgctgga ggacgattaa tcggtacgag
    12121 gtttccttca tcatcaaaca acgctacgcc aatattggtg gctatccctg gcccctctcc
    12181 cacggaaagc acatccggat ttttaccatc cgcgacaccg tgaaacgcca cacctacacg
    12241 ttcactcacc accgtgctac attcccgtaa atgaataaca aaaggcaccg gtgcgctatc
    12301 ttcccctacc gcatgaaacc ggttactgct gatttgcccc atattgaccg tcatttgttt
    12361 atcaccggct tcaatccggc aagtttccgc aataatgacg ccctgaaatt gcatatttcc
    12421 gccgggcagc gtggtattcc atttatttcc ggccagagca aacattggca gcaacgacgc
    12481 taatagaaat aatctcttcc ttttcatcgt tatttttatc gcacaagggt gggcatccct
    12541 gcccgtaatg acgtccctga acctgggtag gttattgata ctgaaccttg aaggtcgcat
    12601 ccgcattagc agcacccggg gttgcctcgc cgattgcata ataacgcgcc tggaacggaa
    12661 tggtgttggt accgttattc aaggttgttt gtgcactaaa tgtcgcaccg tccagcgtca
    12721 gctcatttcc tgttctgtcc agtatctgca caccaacatt tgttgcgcta cccgcagctg
    12781 aactctgcag agccagtact ttaggatgag cactgtcaat cgcagtcccc aagaaggcaa
    12841 cagcggcttt tgtggcaaca ctggtatcgc aatcattcag ctgaatatta aaaccgacag
    12901 cagagctggt tgctccagtc tgcttcagac tagcggtacg aacctggccc aactgaacgg
    12961 tttgatcaac agagcctgca tcaactgcgc aagcggcgtt aacaacttcc cctttaaagt
    13021 gaacggtccc accatttacc gtcgtgggtg tagtttcagc cagagccgct gcggaactga
    13081 gggacagagc cgacagaaca acgattgcca gagttttaat tttcatgctg ctttcctttc
    13141 aaaaaactat ttctaaatcg acatgggcag tcgttctgta cactttgttt tgtcaacgag
    13201 tttgactgcc aacactgcac agttttcccc caaaagatga aacatttggg gccattttga
    13261 ctcatagagg aaagcattgc gcacaaactt tttcagttaa tttgttggct taatattcta
    13321 ttgttatctt tatttataga tttttatatt gcatgaggtg gtttttggag agaagaatga
    13381 ggaagaggcg tcgagccaca gaaa
//
LOCUS       DAZKFU020000125        13351 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZKFU020000125 DAZKFU020000000
VERSION     DAZKFU020000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.126"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..397)
                     /gene="yhaJ"
                     /locus_tag="ACHOQQ_RS14615"
                     /old_locus_tag="pgaptmp_002923"
     CDS             complement(<1..397)
                     /gene="yhaJ"
                     /locus_tag="ACHOQQ_RS14615"
                     /old_locus_tag="pgaptmp_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU4471143.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITE"
     gene            502..1203
                     /locus_tag="ACHOQQ_RS14620"
                     /old_locus_tag="pgaptmp_002924"
     CDS             502..1203
                     /locus_tag="ACHOQQ_RS14620"
                     /old_locus_tag="pgaptmp_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGU4471144.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLRKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1226..1390
                     /gene="yhaL"
                     /locus_tag="ACHOQQ_RS14625"
                     /old_locus_tag="pgaptmp_002925"
     CDS             1226..1390
                     /gene="yhaL"
                     /locus_tag="ACHOQQ_RS14625"
                     /old_locus_tag="pgaptmp_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGU4471145.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1524..2834)
                     /gene="cyuA"
                     /locus_tag="ACHOQQ_RS14630"
                     /old_locus_tag="pgaptmp_002926"
     CDS             complement(1524..2834)
                     /gene="cyuA"
                     /locus_tag="ACHOQQ_RS14630"
                     /old_locus_tag="pgaptmp_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGU4471146.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVVEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2862..4193)
                     /locus_tag="ACHOQQ_RS14635"
                     /old_locus_tag="pgaptmp_002927"
     CDS             complement(2862..4193)
                     /locus_tag="ACHOQQ_RS14635"
                     /old_locus_tag="pgaptmp_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4471147.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4468..5832)
                     /gene="tdcG"
                     /locus_tag="ACHOQQ_RS14640"
                     /old_locus_tag="pgaptmp_002928"
     CDS             complement(4468..5832)
                     /gene="tdcG"
                     /locus_tag="ACHOQQ_RS14640"
                     /old_locus_tag="pgaptmp_002928"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4471148.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5904..6293)
                     /locus_tag="ACHOQQ_RS14645"
                     /old_locus_tag="pgaptmp_002929"
     CDS             complement(5904..6293)
                     /locus_tag="ACHOQQ_RS14645"
                     /old_locus_tag="pgaptmp_002929"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGU4471149.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6307..8601)
                     /gene="tdcE"
                     /locus_tag="ACHOQQ_RS14650"
                     /old_locus_tag="pgaptmp_002930"
     CDS             complement(6307..8601)
                     /gene="tdcE"
                     /locus_tag="ACHOQQ_RS14650"
                     /old_locus_tag="pgaptmp_002930"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGU4471150.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8635..9843)
                     /gene="tdcD"
                     /locus_tag="ACHOQQ_RS14655"
                     /old_locus_tag="pgaptmp_002931"
     CDS             complement(8635..9843)
                     /gene="tdcD"
                     /locus_tag="ACHOQQ_RS14655"
                     /old_locus_tag="pgaptmp_002931"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGU4471151.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9869..11200)
                     /gene="tdcC"
                     /locus_tag="ACHOQQ_RS14660"
                     /old_locus_tag="pgaptmp_002932"
     CDS             complement(9869..11200)
                     /gene="tdcC"
                     /locus_tag="ACHOQQ_RS14660"
                     /old_locus_tag="pgaptmp_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGU4471152.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11222..12211)
                     /gene="tdcB"
                     /locus_tag="ACHOQQ_RS14665"
                     /old_locus_tag="pgaptmp_002933"
     CDS             complement(11222..12211)
                     /gene="tdcB"
                     /locus_tag="ACHOQQ_RS14665"
                     /old_locus_tag="pgaptmp_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGU4471153.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVARPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12310..13248)
                     /gene="tdcA"
                     /locus_tag="ACHOQQ_RS14670"
                     /old_locus_tag="pgaptmp_002934"
     CDS             complement(12310..13248)
                     /gene="tdcA"
                     /locus_tag="ACHOQQ_RS14670"
                     /old_locus_tag="pgaptmp_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGU4471154.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3478 a         3583 c         3140 g         3150 t
ORIGIN      
        1 cttctgtgat gattgccagt tgggtattgg cttttgccgc cagtttgtcg attaacggga
       61 aaaaggcagg tgtcggtacc agcgcttcgg tcacaatggt gagatgcgtt tcccaaccgc
      121 gcgcgagagc ttccgcatca gtagtcagtt tatctgcggc ttccagcaaa acgcgccctc
      181 gctccaacaa catcctcccg acattggtga atttggtgcg atggcccgag cggtcaaaca
      241 gcaccacatc cagctcttct tccagttttt gcatggtgta gctaagtgcg gaaggcacgc
      301 gtcccagctc atccgctgcc gccgcaaaac tgccccggcg atcgatcgca tccataaccc
      361 gtagtgcttc cagcgttaat gccctttctt tggccatttc gttctcattc aggaaatttg
      421 aacataccgg gcagaatatc tggctaacaa tgtagcgtcc agccccttac cataaaagga
      481 agtaaagaga ggtcaagaat tatgattact acccgaactg ccaggcagtg tggacaagca
      541 gactacggat ggttgcaggc ccggtatact ttttcctttg gacactactt cgacccgaaa
      601 ttgttaggct atgcctccct gcgtgtgctt aaccaggaag tgctggcccc aggtgccgcc
      661 tttcagccgc gaacctatcc caaagtcgat attttaaatg tgattctgga tggagaagca
      721 gagtatcgcg acagcgaagg caatcatgtt caggccagcg ccggtgaggc gttgctgctc
      781 tctacccagc cgggtgtcag ctacagcgaa cacaatctcc gcaaagacaa accgttaaca
      841 cgaatgcagc tttggctgga cgcctgcccg cagcgagaga atccgctgat tcaaaaactg
      901 gcgcttaata tgggcaagca gcaattaatc gcctcgccag agggtgcgat gggaagcctg
      961 caattacgcc agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga aagtgcgaat
     1021 ttccagttgc atgggccacg cgcgtatttg caatcgattc acgggaaatt tcatgcgctt
     1081 acgcatcatg aagagaaagc ggcgctgacc tgcggtgatg gggcgtttat tcgtgacgag
     1141 gctaacatta cgctggttgc cgattcccca ctgcgcgctt tgctgataga tttgcctgtc
     1201 tagttgtttt tacagggaga tgatgatgag taaaaaatcg gccaaaaagc gccagccagt
     1261 gaagcccgtg gtggcgaaag aacctgctcg caccgccaaa aattttggct atgaagagat
     1321 gttgagcgag ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg aggatgaagc
     1381 taccgcgtaa agacaggttg ccggatgcgg cgaaaacgcc ttatccggcc tacaaaatcc
     1441 aacaacatta acgaattaaa caacccgtag gccggataag atgcgccagc atcgcatccg
     1501 gcacgatacc caaaacctgg cgtttatctg gccttgctcg ccataatctc gataatctgc
     1561 cgatccgttt gctgcatcga atggcttgct aatgcacaca ggttggcaat cgactgctca
     1621 acatcatgcg ccacaatccc ttcattgccg gtcacggcgg tatcatccag cgccattaac
     1681 accgctttcc acgcagccga agcactggtc gaaaccttca tcgcgcagct gttcgacgca
     1741 ccatcgcaaa tcatgccgct gacatcgccg atcatactgc tgatcgccat cgagatggtt
     1801 tcataacgcc catccaccag ccatgccatc ccggcggcgg cccccattgc tgcggtcgtt
     1861 gcggcacaca gcgcagacaa acgcggtaac tggttatgga tgtaaattgc gctcaaatga
     1921 gaaagcatca gcgcacgcgc cagccgttca tcatccgctc cgaagtgttc tgctaccacc
     1981 accacaggca ttgttgcggt gatcccctgg ttacccgagc cggagttact catagccgga
     2041 agcgtagcgc cgcccatacg cgcatcggat gccgcgctgg tacgaatcac aatggatgaa
     2101 gagagatctt tcgccagcaa accgcgcgcg cactgttttt ccagcgtcgc gccaatatgc
     2161 agcccccact taccgctcaa accttcctgc gataacgcgc aatttagctt cgcggaatcg
     2221 agaataaagc ggatcgccgc aaacgggact tcattgacga acttcaggat ctcagccagc
     2281 gtcgttctgg aaagcaccgt cagcggagac tcttgctcgc cctctaccac acacgcctgc
     2341 tgggtaaaca ccacaccatc gtgcgtctca atatgcacaa tgttggtatg cccgccgacg
     2401 atggtgacac acgcccactt ctcaccgttc cagactttgg cgcgtgagaa gaggatttca
     2461 ttgcaaggtt cctggatctt aacggagact ttccccgccg ccagcagtgc tttggcatcg
     2521 gcaattgcct gcgcagttgc gtctttcagc acttccagcc cggcgttggc atttccacct
     2581 aacgccccca gcgccgccgc aatcggcagc cccaccattc ccgtgccggg aacggtgacg
     2641 cccagaccgt tcttcatcag atttggcgaa acccaggctt ctacacgttc aaccggacct
     2701 tccagttctg ccgcagcaac cgccgccgcc agcgccagtg aaatcggttc agtacatccc
     2761 agcgccggtt ttacttcctc ctgaacggcg aggatgtaac gctgccataa cggatttaaa
     2821 gtcgaatcaa acatattaaa aaccttaaaa tttcaggtaa atcaagaaaa tgccaggaac
     2881 ggagaaacac aaagcaacaa accagtgacg ataatcaggt acagagacat ccctttgtat
     2941 ttgtgcaatg ccggtacttt gtaaaccagc cacgccggga tcaggcaccc taccatgccg
     3001 aaaatcgggc tacagataga ggtgaagctc aacaccggtg cgttcagtac gatggcgctc
     3061 caggccagca aaatggcgaa aatcatgatg ccgcgctgaa caagattttc gttaatcttc
     3121 tcggcaggca tcttgcgacg caggatgttc attacgatcc cttgcgttgc ttcgcgaaag
     3181 cctaaataga cgccaaagaa tgcagtcatg acagcaaaga tattgagaat gacgctgacc
     3241 actttcaccc acgctgcgcc gtcaccgcta ataaactgtg cggcaatcgc cagcgcggaa
     3301 atattctgct cgtaggcttt taccgcttcg tcatgtccca tcgccagcgt gaacgacacg
     3361 gcgtagaaaa agacggtgac aaacaaaatg ccaaacgcga tattcattgc ccgcaatgct
     3421 ttatgccgcg ccacttcaat tgatttttcc cgcgagcgat aagagatcac catcgggctt
     3481 aacgtctgga taaacagaat cgacgtcagg gtaaacggca gcgtaataat ggcgtttttc
     3541 accagcagcc ccagcggcgg tagcgaaccg acgttataca gatgccacat tcccaccatc
     3601 gacacgccca gcgccgcgac caccagcagc tttgtcagca ccatgccagt cgaaattttg
     3661 aataacaatt tctcgccgcg tgaagagatc gccaccagaa tgcaaatcag taccagacca
     3721 taaaagggac tgtctgacag caacccttcc gtcacgccga aggtatgcag gtaggaagca
     3781 ctatcgttgg tgatggcggt ggaatagacg aacatccaaa tcaccagcat tacgaaatag
     3841 agtgcaccta acaggatgcc ccagttttta cctaaataac cgctaatgac gctcgggtaa
     3901 tctttacatt ctggtgattc tgccagcgta ttaataaaca accgctgaaa cagatacatt
     3961 gccgggtaac caatcaccga tgagagcaaa aatacccaca atcccatcaa accgacctgc
     4021 accgggagaa aaacaatccc cgcgccaatc gccatcccga tactcataat cacccagccg
     4081 gtgtcagtac tgtcgaattt gatcgcttct cgccactcgc tctcactcat tccagcacga
     4141 cccgccgggg tcgaagcgtc tgcaatgacg cctttattcg atgcaatttc cataatttct
     4201 cgctcaatat tttgtagggc ttattatttt tttccgagcc gcatcaaggc gatatgcggt
     4261 ctacgttttt gcaggcgagt tattagaata aagaaatgat acgcgcgagg tcagaaaaaa
     4321 tcttcacaat caagactttg aaataacgga gatggataag aattttctac taaattaatc
     4381 gcaagagaat attcattctc tatttgcggc agatcacaaa aaaaaggtgc acatttgtgc
     4441 acccaaggat gaaagctgac agcaatgtca gccgcagacc actttaatgg ccagtcctcc
     4501 gcgtgatgtt tcgcggtatt tatcgttcat atctttgccg gtttcataca tcgtctcgat
     4561 cactttatcg agtgaaacac gcggtgccga ggtgcggcgc atcgccatcc gcgcggcgtt
     4621 tactgctttc acggcattaa tggcattacg ttcaatgcac gggatttgta cctgtccggc
     4681 aaccggatcg caggtcagtc caaggttatg ctccatcgcg atttccgccg cattgcatac
     4741 ctgcgccgga ctgccgccca gtagttcagt taaccctgcc gccgccattg aacaggccac
     4801 gccaatctcc ccctgacagc cgacctccgc gccagagatg gaggcgttca ttttatacag
     4861 cgcgccaata gccccggctg caagaaaata gcgggcaatt gaccgctcgt ttaccggacg
     4921 acggaactta tcgtaataag ccagcactgc cggaataatg ccgcacgcac cgttagtcgg
     4981 tgccgttacc acgcgcccgc cagctgcgtt ttcttcacta accgccagcg cgtacatgtt
     5041 gatccagtcg atgacattca tcggatcgtt agagatgtta tcgctggaaa ccagttgacg
     5101 acgcagcgct acggcacggc gcggcacatt gagcggacca ggcagcacgc cttcagtgtt
     5161 catcccgcgt tcaataccgt catgcatcac ttgccagata cgggcaaaac cggcgtcaat
     5221 ttccgcttta ctgcgcagcg ctagctcgtt gtgcatcatc aggccagaaa tagacaggcc
     5281 gttgtaatca cacattttca gcagttcacc tgctgagtgg aaatcgtaag gtacggacgt
     5341 ttcgacatcg tgcgacaggc cgaagtgttc ttcttcgaca ataaacccgc cgccgacaga
     5401 gtaataggtt ttacttaata gctcttcctg tgccttccag gcagtgatcc gcattccgtt
     5461 ctcatggcga ggcaacattt cgggatggaa gataatgttc tttgctacag gaaaatcaac
     5521 aatatgcgca ccagatgcca ctggcagccg cccgctgcgc gttactaact ctataaatgc
     5581 agggatctca tcaatgacaa catcctgcgg actgtttcct gccagtccca tgatgatggc
     5641 gacatccgtg gcatggcctt tgcccgtcag tgacaacgac ccgtacagat cgaccacaat
     5701 atggctcgtc gcggttaata agccgctact ttccagccga tcaataaaac tttttccggc
     5761 attcattggc cccacggtat gcgaactgga gggaccaatc ccaattttga aaatatcgaa
     5821 tgcactaatc atatccacac cctcggattg ccgttcagtg aagtggagcg gaacgacctt
     5881 acgaccgtcc cgctcacgag gctttacgca ctacgtactg cgatagcttc aatttccagc
     5941 ttcacatctt tcggcaaacg cgcgacctgt acacagctcc gggtcgggta ggtcgcctga
     6001 tgctcatcga agaactgctt atagacttcg ttgatggtgg caaaatcatt cagatcggtg
     6061 atgaacacgg tcatcttgat gatatcgccc acgctcagcc cggcagcaac cacgatcgct
     6121 ttgacgtttt cgaggcttaa acgcgcctga tcttgcacat cagccgggat ctcaccggtc
     6181 tgtgggcaaa ccggaatttg accggaggtg aagaccatgc tgcctaaatc aacgccttga
     6241 acataagggc cgattgcgcc tggggcacgt tgcgtttcga taatcttttt catacatcct
     6301 ccggcgtcag agcgcctggg taaaggtacg tgaaataaca tcctgctgct gttcacgggt
     6361 cagtgcgttg aagcgcacgg cgtagccaga gacacggatt gtcaggttag gatatttttc
     6421 cgggtgctcg atggcatcca gcagcatttc ccgattcatt acgttgacgt tgaggtgttg
     6481 accgccttcg acatccgctt cgtggtggaa atacccatcc agcaggccga caaggttggt
     6541 tttacgtact ggatcttctt tgcccagcgc cgcagggacg attgagaagg tgtacgagat
     6601 cccgtcttta gcgtaggtga acggcagttt cgccaccgac gtcaatgagg ccacggcacc
     6661 tttgcggtca cgaccatgca tcgggttagc gcccggcgcg aacggtgttc cggcgcgacg
     6721 accgtccggc gtattaccgg ttttctggcc gtacaccacg ttagaagtga tagtcagaat
     6781 cgactgggta gggacggcgt tgcgataggt tggcagcgct ttaattttct tcataaaacg
     6841 ttcaaccagg tcgcaggcaa tgctgtctac gcgctcgtcg ttgttgccgt actgcggata
     6901 ttcaccgtcg atttcaaagt ccaccgccag gccgttttcg tcacggattg gtttcacgcg
     6961 ggcatatttg atcgcagaca gggagtccgt tgccaccgac aggcccgcga tgccgcatgc
     7021 catggtgcga tagacatcac gatcgtgcag cgccatcagc gaagcttcgt agctgtactt
     7081 gtcgtgcata tagtgaatga tattcagcgc actgatgtac tgcaccgcca gccagtccat
     7141 gaagtgatcg aggctgtcca tcactttgtc gtaatccagc acatcgtcca tcaacggtgc
     7201 tgttttcggc ccgacctgaa tcttcagctt ctcgtccacc ccgccgttaa ttgcgtagag
     7261 cagcgttttc gccaggttag cgcgtgcacc aaagaactgc atttgcttac caatcaccat
     7321 tgggctgacg cagcaggcaa tcgcgtaatc gtcgctgttg aagtcagtac gcatcagatc
     7381 gtcgttttca tattgcaagg aagaggtgac gatcgacacc tgcgcggcat attttttgaa
     7441 ggcaatcggt aattcttccg accaaagaat ggtcaggtta ggttccggtg caggccccat
     7501 agtgtgcagg gtgtgcaaat agcggaagga gtttttggtc accagcgtac gaccgtccag
     7561 ccccatcccg ccgatcactt ccgtcgccca gattgggtcg ccggagaaca gcgaatcaaa
     7621 ttccggtgta cgcaggaagc gcaccatacg gatcttcatg atgaagtgat cgatcagttc
     7681 ctgtgcctgc tgctcattga gtacgccagc tttaaagtcg cgctcaatgt agatatccag
     7741 gaacgatgcc gtgcgaccca gcgacatcgc gccgccgttc tgtgatttca ctgccgccag
     7801 ataagcgaag tagagccact gcaccgcttc ctgcgcattc tgcgccgggc gagagatatc
     7861 aaagccatat ttcgccgcca tttcctgaat ctgcaacagc gcatgacgat gctctgccag
     7921 ctcctcacgc agacggatgg tggcttccag atcctcgcct ttttccagac gagactggag
     7981 atcggcaaat tgcagttcgc gttcacgcac cagataacga atgccataca gcgctacgcg
     8041 gcgatagtca ccgataatgc gcccacgtcc ataaccatct ggtaaaccgg tcagtacgcc
     8101 agatttacgg cagcgcagca tatccggtga gtaaacatca aatacgccct ggttatgggt
     8161 tttacgcaga tcggtaaaca gatattcaaa ttcactgtcc atttcacggc cataggcgtg
     8221 gaatgaactt ttaatcatat taatgccacc gaacgggtgt agcgcacgtt tcaacggcgc
     8281 atccgtttgc aggccaacaa ttttttccag cggctggtta atatatcccg catcatgagc
     8341 ggtaattgtg gtggcaatat tggtatcgaa atcaaccggc gcgtgggttg cgttttcgat
     8401 acggatgcct tccatcacct tttcccacaa ttccgtggtg gcaggcgtcg cttcggcgag
     8461 aaaagattca tcaccttcat acggtgtata gttatgttga ataaaatcgc ggacattaat
     8521 ttcgcttttc cagtccgtac ctttaaagcc aagccatgcg tcggcgtaca gcttatcgct
     8581 ggtatcaata tctaccttca tgaaaaataa tctctctaca atacttcaac taaattatgc
     8641 aaattctgcg ggcgcgttaa ctttgcctaa atgaatggca tccaaagcaa tcattttttc
     8701 ttcgttagtc ggaataacgg cgcaaatgac acgcgcattt tcactggaaa caattcgctc
     8761 gccaaaagag ttagatcgat tattcatttc tgtatcaatc tctacgccta atacagccaa
     8821 atgttccatg accagacgac gaattaaact tgaattctct cctattccgc cggtgaatat
     8881 aattccatcc aggcgacgta atgaagctgc gtgtccggca atatgacggg caattcggtg
     8941 aacaaaggtt ttaattgcca gctgcgcgcg ttcgtgacct tcatgccagg ctttttccag
     9001 aacacgtaaa tccgaagaaa gaccggaaat acctaataat cccgactctt tattcactac
     9061 gcgttccagg tcacccaggc tctggttggt ttggctggcg acccaggaca tcgcaccaaa
     9121 gtcgacatcg ccactgcggg tacccatcat caagccttcc agcggcgtca ttcccattga
     9181 ggtatcaaca ctctgaccgt tgcgaaccgc gcagattgac gcgccattgc caagatgcgc
     9241 cacaaccagg ccggagtcat cttccgccag attcagcagc gaatgtgcgc gctgggaaac
     9301 atagcggtgc gacgtgccgt ggaaaccata acggcgtaca cctaactctt cataatattt
     9361 ccacggcagg ccgtataaat aagcttccgg agccatcgtc tggtggaaac tggtatcaaa
     9421 taccgccacc tgagttacgc ccggaaataa ttgctgcgcc gattcaatac cacttaaatt
     9481 ggcgtaatta tgcaggggtg ccagtggaga aacgcgacgg atattatcaa tgacttcatc
     9541 ggtaataatg gcggactcgg taaaaatact gccgccgtga gcgatgcggt ggccaattaa
     9601 ggccacgctg tcatttaaat tccgtttttc cagttcaaat gcaattgcct tcaatgcacc
     9661 ttcgtagctg tggtgagcca gcggtgctgg ctctccccca tttacggata agaatgcatt
     9721 ttccgagtta ataccgtcgg caatacctga cattaatact tcacagtcgc tggcatcgag
     9781 tacggaaaac ttaatcgaag acgaaccaca gttaataacc aaaacaaccg gaaattcatt
     9841 catctctttt ctcatcctga gttacggatt aaaacagttt gtatacgatg ttcaggatgg
     9901 tcagcagacc aatcacggta acaaacacgt tatccagacg accacggtat ttcgccagag
     9961 acggcgcttt acggatggca tacatcggca acaggcacag cagggatgcg ataatcggtg
    10021 cgcccatggc ttcaatcagg tcgaggatgt tcgggttggc gtaggcaaca acccaagtgg
    10081 agcccatgat gaagatcatg ctgagagtat tcagtttacc cagcgacact ttggttttgt
    10141 cacctttata accgaacttc agaatcagac cattcaagcc ttccagcgtc cccagatagt
    10201 gaccgaagaa agatttgaag atagccacga gtgcgatgat ggaagccgca tattccagtg
    10261 taattgcgaa cgttgttttc gtaccggtca tggacgcaaa gtggttagcc agataagaaa
    10321 gcactggaat attctgcgct ttggcttccg ccatgttggc cggagacagc gtaaacaggc
    10381 agctaaaggc aaagaacatc accactgcaa ccatcagcat gctggcacga gaaatgattt
    10441 gggaacattt acgttcggtg aagtcgcgac cgaagtcttt ctcatactct tcacgtttag
    10501 aaaccacgaa ggaagagacg attggcgaga agttaaagga gaaaaccatg atggaaatcc
    10561 ccagccagac agtgatcagg ataccgtcat gaccggttaa cgacagcgaa ccgaggtcaa
    10621 cctggtcgat aactgcagag ttccagtaag ggatcagcga caaagaaatc agcaccaggc
    10681 tggcgataaa cggccatacc aggtagctca tcactttaac catcagatcc ttaccaaacc
    10741 agatgacgaa agccatcagc agcaacagga acagcgccac aaagccgcga ttcagcggtg
    10801 caaagccgag ctggttttcc cagaacgtca taaaggtatt ggtaatagta acgccataaa
    10861 tccacagcag tgggcaaatc gcgaagaagt acaggaacgt gataaccacg ccgccagttt
    10921 taccaaaatg ctcttccacc gtttccgtaa tgttgccgga aggattagag ccagaaagac
    10981 acagacgcgc cagcgcccgg tggcaataaa acgcgatggg gtatgccaat accaacatca
    11041 gaagaatcgg gatcagtccg ccaaaacctg cgcggatagg gaagaacagc accccggcgc
    11101 cgattgccgt accaaacaag cctaacgtcc atgtggtatc tgatttacgc caggacgatt
    11161 gttttgtctg gctggataca atgctatctg aagtactcat atcctatcct caacgaatta
    11221 attaagcgtc aacgaaaccg gtgatttgag agacgcgaga aagatcgata ttgccgccgg
    11281 aaataatact gacggttttt ctgttttgaa tatattggtc taatttaccg cttaataatg
    11341 cagcacatgc cagagcgcct gcgccttcgg tgacgacttt attgcgctga attaaggcaa
    11401 tcatactgtt tctgatttcg tcttcgctga ccagcacgat gtcatcgact aattcacgaa
    11461 cgatttcgta agttaaatta cccgggcggg cgacatcaca accatccgcc agggtgccgg
    11521 tagttcggtg cgtggttatt tctccggagt ggaaagaagc cgccatgccg tgaacgtttt
    11581 ctgactgtac gccaataaca cgaatggtcg ggttaataga tttaattgcc accgcaatac
    11641 cagcaattaa accgccacca ccaattggca caatcacgtt atcgacatca tagagatctt
    11701 ccataatttc caggccaatc gttccctgac cagcaatcac tttcggatca tcgtaaggtg
    11761 ggataaaaat acggccttcc atttcgacaa tttcgctcac tttagcgata gtgtcgttga
    11821 agttatcacc atgcagaacg acttctgcgg agtagtcgca cgttgccgct actttggatt
    11881 ttggcgcacc ttttggcatc accactttac cgtcgatacc cagcatcgcg caggagaggg
    11941 aaaccccttg cgcatggttg cccgcagaac aggccaccac gcctttgcgt ttttccgcat
    12001 cggtcagtga acttaattta ttaaatgcgc cacgaatttt aaatgaaccc gtacgctgca
    12061 tattttcaaa cttcaggaat atttcacctt tgcaacgttc actaaaatag ttggagcgag
    12121 gcatacctgt tttataaatt cgcccagcca gtcgttgttt cgcttcaata atgtcatcaa
    12181 tagcaaccgg cagatcgtat gtaatatgca ttataaaacc tcttcgcctg aattaaatat
    12241 gtaggtaacc gtaaccgaca cctgcaagac agacaggtgg attatttatg tttaaataaa
    12301 aacaaataac taaccaactt ctattaattg ccttcgtcta cacccattat aagatgaata
    12361 ctctttggct aattccacca aaaccgatgc tgctttttta atacgataat ttttcgacca
    12421 taccgcggca tattgtgcca caggtaatgt ttcttcaacc ggaatagtaa taaattgatt
    12481 agaaccaaaa ggtgacgtca tatcacaagg aattacagtt aagaaatcag cattgagaac
    12541 aagattataa attgtcacga ctgagtcggt tttaacgatg ttttcaatac tgatgccatt
    12601 tctttgtaac gtagtaagca gttcgctgta gtaccccata ttggtttgtg gcaacaccca
    12661 ctgttcgttc ttcaacgact ccagcgtggt ggtgccggtg catgttcggg acttactggc
    12721 taccagcaca aactcggact cgaacagcgg ctcaacatgt aaatcctgaa gcttcatttc
    12781 tgcacttaac gtaccaatcg caaaatccag tcgaccgtcg cggattgccg gtaagaacga
    12841 agacagttgc gcttcataca tagaaacctg cgcttttggg aacacctctt tgaacttatt
    12901 gatcatccct gacataaaag taaaaccaat caatgaagga aaaccaaatg agacttccac
    12961 caccgcctca gaagacatac cgcttatctc attaaccata tttttcattt cacgggtaat
    13021 ggattcggaa cgggagagta acaattgacc ggcaggtgtt aatgttacac cggtattttt
    13081 ccgcaccact aattccacac caaaataatc ttcaatatcg ttaatgattt tactgacggc
    13141 tggttgagtt aaccctaatt cttttgcagc cgagccgata gagccacttc taatgacttc
    13201 ctgaaagact accaggtgct gcgttttcgg aagaagaata gtgctcataa cgacctacgt
    13261 taattacctc attgacggca tgaagtgtat caaaatgaaa tgaacaggat atgtgcgacc
    13321 actcacaaat taactttcaa tactttccag g
//
LOCUS       DAZKFU020000126        13216 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZKFU020000126 DAZKFU020000000
VERSION     DAZKFU020000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.127"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..301
                     /locus_tag="ACHOQQ_RS14675"
                     /old_locus_tag="pgaptmp_002935"
     CDS             <1..301
                     /locus_tag="ACHOQQ_RS14675"
                     /old_locus_tag="pgaptmp_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HGU4471155.1"
                     /translation="MQGFIRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLS
                     FGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
     gene            complement(442..768)
                     /gene="ypeC"
                     /locus_tag="ACHOQQ_RS14680"
                     /old_locus_tag="pgaptmp_002936"
     CDS             complement(442..768)
                     /gene="ypeC"
                     /locus_tag="ACHOQQ_RS14680"
                     /old_locus_tag="pgaptmp_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGU4471156.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(883..2140)
                     /locus_tag="ACHOQQ_RS14685"
                     /old_locus_tag="pgaptmp_002937"
                     /pseudo
     CDS             complement(883..2140)
                     /locus_tag="ACHOQQ_RS14685"
                     /old_locus_tag="pgaptmp_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
     gene            2344..3309
                     /gene="glk"
                     /locus_tag="ACHOQQ_RS14690"
                     /old_locus_tag="pgaptmp_002938"
     CDS             2344..3309
                     /gene="glk"
                     /locus_tag="ACHOQQ_RS14690"
                     /old_locus_tag="pgaptmp_002938"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGU4471157.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPIEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            3528..3854
                     /locus_tag="ACHOQQ_RS14695"
                     /old_locus_tag="pgaptmp_002939"
     CDS             3528..3854
                     /locus_tag="ACHOQQ_RS14695"
                     /old_locus_tag="pgaptmp_002939"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGU4471158.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            3876..5123
                     /locus_tag="ACHOQQ_RS14700"
                     /old_locus_tag="pgaptmp_002940"
     CDS             3876..5123
                     /locus_tag="ACHOQQ_RS14700"
                     /old_locus_tag="pgaptmp_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGU4471159.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            5138..6223
                     /gene="ypdF"
                     /locus_tag="ACHOQQ_RS14705"
                     /old_locus_tag="pgaptmp_002941"
     CDS             5138..6223
                     /gene="ypdF"
                     /locus_tag="ACHOQQ_RS14705"
                     /old_locus_tag="pgaptmp_002941"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4471160.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLATIVNEIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSTESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            6223..7260
                     /gene="ypdE"
                     /locus_tag="ACHOQQ_RS14710"
                     /old_locus_tag="pgaptmp_002942"
     CDS             6223..7260
                     /gene="ypdE"
                     /locus_tag="ACHOQQ_RS14710"
                     /old_locus_tag="pgaptmp_002942"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4471161.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            7285..9780
                     /gene="ptsP"
                     /locus_tag="ACHOQQ_RS14715"
                     /old_locus_tag="pgaptmp_002943"
     CDS             7285..9780
                     /gene="ptsP"
                     /locus_tag="ACHOQQ_RS14715"
                     /old_locus_tag="pgaptmp_002943"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4471162.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGEIRYLPLL
                     LGLDLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVIGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9783..10640)
                     /locus_tag="ACHOQQ_RS14720"
                     /old_locus_tag="pgaptmp_002944"
     CDS             complement(9783..10640)
                     /locus_tag="ACHOQQ_RS14720"
                     /old_locus_tag="pgaptmp_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4471163.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRENVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(10653..11387)
                     /gene="ypdB"
                     /locus_tag="ACHOQQ_RS14725"
                     /old_locus_tag="pgaptmp_002945"
     CDS             complement(10653..11387)
                     /gene="ypdB"
                     /locus_tag="ACHOQQ_RS14725"
                     /old_locus_tag="pgaptmp_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGU4471164.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(11402..13099)
                     /gene="ypdA"
                     /locus_tag="ACHOQQ_RS14730"
                     /old_locus_tag="pgaptmp_002946"
     CDS             complement(11402..13099)
                     /gene="ypdA"
                     /locus_tag="ACHOQQ_RS14730"
                     /old_locus_tag="pgaptmp_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGU4471165.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         3106 a         3400 c         3606 g         3104 t
ORIGIN      
        1 gatgcagggc ttcattcgct ttcatatccc gctgtgggtg cgtcgtacag tcaccatgct
       61 gccgtcattt attgtcattc tgatgggatt agatccgaca cggattctgg ttatgagtca
      121 ggtactgtta agttttggta tcgccctggc gctggttcca ctgctgattt tcaccagtga
      181 cagcaagttg atgggcgatc tggtgaacag caaacgtgta aaacagacag gctgggtgat
      241 tgtagtgctg gtcgtggcgc tgaatatctg gttgttggtg gggacggcgc tgggattgta
      301 gttgaatgag cgtcgcatct ggcactattg gcggatgcgg cgtaaacgcc ttatccgccc
      361 tacgcgttaa agagccggtt tgtatgcctg ataagacgcg ctagcgtcgc atcaggcatt
      421 gtgctccatt ggcgaatgtg tttaatggcg gtgtccatgt ccgcggcctt ttccgcgatg
      481 atcgtcacga tcgcgccagc cttcacgata gccacgctca tatgctttac gcttatccca
      541 gccacgatgg tagccattat catgacgcca ccagcggttt ttacgccatt cataattgcg
      601 atgccagtag tcacggtcgc gccagtgacc accgtcccag taattaccgt aatgatcgcg
      661 atcgccaatt tgtaatttga ttgatggcag tagggtgatt tcgcctgcgt ttgctgcaag
      721 cggggtaaat gccatcaggg cggccgccag aaacagtgac ctgaacattg ttattctcct
      781 tcacgatcga agccgtcagc ggcctgttaa cgcaatatta cggggaggta aagccccgta
      841 tcatcgccat aactcttaaa tcataaagga gagcattttt tgctaaacgt tatcgtgggg
      901 ggggctgttg cttcggacga ttgaccatca tcatcggctt acctgctaat aacagccatg
      961 ccggaagcat gacgatacag agcagcggta gcaatgtggt attgggtaca acgactgccg
     1021 ccataaaaag gcttaaccag ccatcgcgtg ttaccaccag cacgatgccg agaatagcgc
     1081 aggaaacggt tattgccgct ggaacggcgg ggacgtgctc atgcagcatc aaccctaatg
     1141 ccacgccgac gaacactgcc gggaaaattc gtccaccgcg aaagccactg gcggcagcaa
     1201 cgaccagggc ggtcagttta atcaccgcca gcaaaaagta atcgctggtg ctgaaagcct
     1261 gatttgccac catctgctgc atctcatcca gtcctttaaa cagcgaaacc ggcccaccaa
     1321 taacccccag aataccgaga ataaatccgc caatacccag cacgagcacc ggatttttca
     1381 tttgatgcat catcgcgtgc aaccgtggta agcaccatac ggcgaccatc cctgctgcga
     1441 tggcaatcgc cgcgacaatt gcaccgctga gaatatcggt catttccatc tgtccgtaat
     1501 gcgcaatggg cagtgaaaaa tggggatgga aaaataatcc ggtagtaagt gcaccagctg
     1561 ctgccgccat taacggcgca aaaagacgat cccatagcgg aacttcacta ctgccattta
     1621 aggtttgcga aaatatcaac gccgccgcaa caggtgtacc aaacagcgcg ccgatggttc
     1681 cggcagaggc taaaatagtc cactccattc ggttgacgcg cggtaacaga cgagcgccaa
     1741 tggcaaccgc aagggcgata ttgacggtca tgatcggatg ttccggcccc aggctgacgc
     1801 cgccagcaag accgagaatt aatgctacga taagtccagg tagcgcagag ggcggaaccg
     1861 gtgcgccgat cagcggttca caggcggggt ctggtccggc atgaccctgg ctgaaacgga
     1921 taaccaaccc caccgcaata cccgttagcg ttaatacacc gatgatccag aggggtgaat
     1981 cctgggctat ccccagagtt cccggcagtc gttgccagag caaattctgt aataccgagg
     2041 cgattttcat caccacaatc agaataagac tggacgcaat cccaattgcc acggcgggga
     2101 gcgataataa caacatggtt ctggctcgcg gatggagcat gttctattcc ttatgcgggg
     2161 tcagatactt agtttgccca gcttgcaaaa aggcatcgct gcaattggtg ctgaaacgat
     2221 aaagtaattg tgtgacccag atcgatattt acagggagcc tgcctttccg gcgttgttgt
     2281 tatgccccca ggtatttaca gtgtgagaaa gaattatttt gactttagcg gagcagttga
     2341 agaatgacaa agtatgcatt agtcggtgat gtgggcggca ccaacgcacg tcttgctctg
     2401 tgtgatattg ccagtggtga aatctcgcag gctaagacct attcagggct tgattacccc
     2461 agcctcgaag cggtcattcg cgtttatctt gaagaacata aggtcgaggt gaaagacggc
     2521 tgtattgcca tcgcttgccc aattaccggt gactgggtgg caatgaccaa ccatacctgg
     2581 gcgttctcaa ttgccgaaat gaaaaagaat ctcggtttta gccatctgga aattattaac
     2641 gattttaccg ctgtatcaat ggcgattccg atgctgaaaa aagagcatct gattcagttt
     2701 ggtggcgcag agccgataga aggtaagcct attgcggttt acggtgccgg aacggggctt
     2761 ggggttgcgc atctggtcca tgtcgataag cgttgggtaa gcctgccggg cgaaggcggt
     2821 cacgttgatt ttgcgccgaa tagtgaagaa gaggccatta tcctcgaaat actgcgtgcg
     2881 gaaattggcc atgtttcggc ggaacgcgtg ctttctggcc ctgggctagt gaatttgtat
     2941 cgcgcaattg ttaaagccga caaccgcctg ccagaaaatc tcaagccaaa agatattacc
     3001 gaacgcgcgc tggctgacag ctgcaccgat tgccgccgtg cgttgtcgct gttttgcgtc
     3061 attatgggcc gttttggcgg caatctggcg ctcaatctcg ggacatttgg cggcgtgttt
     3121 attgcgggcg gtatcgtgcc gcgcttcctt gagttcttca aagcctctgg tttccgtgcc
     3181 gcatttgaag ataaagggcg ctttaaagaa tatgtccatg atattccggt gtatctcatc
     3241 gtccatgaca atccgggtct tctcggttcc ggtgcacatt tacgccagac cttagggcac
     3301 attctgtaaa tccttcctgt tatatcggga gataactctc ccgataatct tttaaatcat
     3361 acagttaatt caatttttct ttgtgtcccc tcacaaggcc gacctgcgtc acacttccgt
     3421 acagcgggat taattctcca gtaaatgcat tatttgtctg gtaacggcga tttgttttgc
     3481 acgttcataa tttcactcgt caactatcac tgaacgagga attaacgatg agtaagaaac
     3541 tgattgcctt atgtgcctgc ccgatgggcc tggctcacac ctttatggcc gctcaggcgc
     3601 tggaagaagc agcggtagaa gccggttatg aagtgaaaat tgaaacccag ggcgcggacg
     3661 gtatccagaa tcgcctgacg gcgcaggata tcgccgaagc gaccatcatc atccattccg
     3721 tggcagttac cccggaagat aacgaacgtt tcgaatcacg cgacgtttat gaaatcactt
     3781 tgcaggacgc aattaaaaac gctgcgggca tcatcaaaga aatcgaagag atgattgcct
     3841 ctgaacaaca gtaatattcg acagggataa ttgttatggc cattaaaaaa cgcagtgcaa
     3901 ccgttgtgcc tggcgcatcc ggtgcggcag cggcagttaa gaatccgcag gcctctaaaa
     3961 gcagcttctg gggtgaactc ccgcagcatg tgatgtcagg gatttcacgc atggtgccga
     4021 ccttaattat gggcggtgtg atcctcgctt tcagccagct gattgcttat agctggctta
     4081 aaattcctgc tgatatcggc atcatggatg ctcttaatag cgggaaattc tccggtttcg
     4141 acctctcctt actgaaattt gcctggttgt cgcagtcctt cggcggcgtg ttgtttggtt
     4201 ttgccattcc gatgtttgct gcatttgtgg cgaactctat cggtggcaaa ctggcgttcc
     4261 cggctggttt tatcggcggc ttgatgtcta cccagccgac gcaactgctg aacttcgatc
     4321 ccagcactat gcaatgggcg acctcttcgc cggtgccgtc caccttcatt ggtgccctga
     4381 ttatttctat cgttgctggt taccttgtga aatggatgaa ccagaaaatc cagttaccgg
     4441 atttcctgtt agcgttcaaa accacatttt tgctaccgat tctttccgct atttttgtca
     4501 tgctggcgat gtactacgtc atcaccccct ttggtggctg gatcaacggc ggtatccgta
     4561 ctgtgctgac tgccgcaggt gagaagggcg cgctgatgta cgcaatgggg atcgccgcgg
     4621 cgacagcaat cgaccttggt ggcccgatca acaaagccgc aggtttcgtt gccttcagct
     4681 ttaccactga ccacgtatta ccggtcaccg cacgttctat cgctatcgtt attccgccga
     4741 ttggtctggg gctggcgacc attattgacc gtcgtttaac cggcaaacgc ctgttcaacg
     4801 cacaacttta tccgcagggt aagaccgcga tgttccttgc ctttatgggg atcagtgagg
     4861 gtgcgattcc gtttgcgctg gaaagcccca tcaccgccat tccgtcgtat atggtcggcg
     4921 cgattgtcgg ctcaaccgcc gctgtctggc tgggcgcagt gcaatggttc ccggaatccg
     4981 ctatctgggc atggccgctg gtcactaacc tgggcgtcta tatggcgggg atcgcgctgg
     5041 gggcagtcat tactgcgctg atggtggtgt tcctgcgtct gatgatgttc cgtaaaggca
     5101 aattgttaat cgatagcctg taagaaggaa aatccagatg acattactcg cttcgctgcg
     5161 cgactggctt aaggcgcaac aactggatgc agtgcttctc tcctcacggc agaacaaaca
     5221 gccgcatctg gggatctcca ccggatcagg ctatgtgctg attagccgtg aaagtgcgca
     5281 cattctggtg gactcgcgct attacgcgga tgtagaagct cgtacgcaag gttaccagct
     5341 gcatttgctt gacgcgacac acacgcttgc aaccatcgtc aatgaaatca ttgccgatga
     5401 acagttgcaa acgctcggtt ttgaaggcca gcaggtgagt tgggaaaccg cgcaccgctg
     5461 gcagtctgaa ctcaatgcga aactggttag cgccacgccg gatgtgctgc ggcaaatcaa
     5521 aacgccagag gaggtggaga aaatccgcct tgcctgtggg attgccgatc gcggtgcaga
     5581 gcatattcgc cgctttattc aggcggggat gagcgagcgc gagatagccg ctgaactgga
     5641 gtggtttatg cgccagcagg gcgcagaaaa aacctctttt gacaccattg ttgccagtgg
     5701 ctggcgtggg gcgctgccgc acggcaaagc cagcgacaag attgttgcag cgggcgagtt
     5761 tgtcactctc gatttcggtg cgctgtatca gggctactgc tctgatatga cgcgcacctt
     5821 gctggtgaat ggcgaagggg tgagcaccga atctcatccg ctgtttaacg tctatcagat
     5881 tgttttgcag gcacagctcg cagcaatctc tgcaattcgc cccggtgtgc gctgccagca
     5941 ggttgacgaa gccgcgcgtc gggtgattac cgaggcagga tttggcgact attttggtca
     6001 caacaccggt catgctattg gcattgaagt acacgaagat ccgcgctttt caccgcggga
     6061 taccacgacg ctacagccag gcatgttact gaccgtggag ccggggattt atttgccagg
     6121 gcaagggggc gtgcgcatcg aggatgttgt gctggtcacc ccgcaaggcg cagaagtgct
     6181 ctacgccatg ccgaaaacag tgttgctcac gggagaggca taatggattt atcgctatta
     6241 aaagcgttga gcgaggcaga tgcgatcgcc tcctcggaac aggaagtacg gcagatcctg
     6301 ctggaagaag cggatcgcct gcaaaaagaa gtgcgatttg atggtctggg atcggtgctg
     6361 atccgcctga atgaatcgac aggtccgaag gtgatgatct gtgcgcatat ggatgaagtg
     6421 ggatttatgg tgcgcagcat ctcccgcgaa ggggcgattg atgtactgcc ggttggcaac
     6481 gtacgcatgg ctgcccgcca gctgcagccg gtgcgcatca ccacccgtga agagtgcaaa
     6541 attcccggtc tgcttgatgg cgaccggcag gggaatgatg tcagcgccat gcgcgtggat
     6601 attggcgcgc gctcgtatga cgaagtgatg caggcgggaa ttcgtccagg cgatcgcgtc
     6661 acgttcgata ccacttttca ggttctcccc caccagcggg tgatggggaa agcctttgat
     6721 gaccgcctcg gttgctatct gctggtgacg ttactacgtg agctgcacga cgccgaactg
     6781 cctgcggaag tgtggctggt ggcaagttcc agcgaagagg tgggattacg cggcgggcaa
     6841 actgccaccc gcgcggtgtc gccggacgtc gccattgtcc ttgataccgc ctgctgggcg
     6901 aaaaactttg attatggcgc ggctaaccat cgccagattg gtaacggccc gatgctggtg
     6961 ttaagcgaca agtcactgat tgcgccgcca aaactcaccg cctggatcga aaccgtggcg
     7021 gcagaaattg gcgtgccgtt acaggcggat atgttcagta acggcggcac tgacggtgga
     7081 gcggtgcact taaccggtac cggcgtaccc acagtggtga tggggcctgc cacccgccac
     7141 ggacattgcg ccgcatcgat tgccgattgc cgcgacattt tgcagatgga gcaactttta
     7201 tctgccctta ttcaacgtct tacgcgtgag acggttgttc aactgacgga tttcagatga
     7261 tctcctgatt aacccggagc ggttatgtta acgattcaat ttctctgtcc tttgcctaac
     7321 ggtctacatg ctcgtccggc gtgggaactt aaagaacagt gcagccagtg gcaaagcgaa
     7381 atcactttta ttaaccatcg ccagaacgca aaggccgatg ccaaaagctc gctggcgctg
     7441 attggcaccg gcaccctatt taatgacagt tgcagcctga acattagcgg cagcgatgaa
     7501 gagcaggcgc ggcgcgtgct ggaagagtac atccaggtgc gctttatcga cagcgacagc
     7561 gttcagccta cgcaggcaga actgacggcg catccgctgc cgcgttcatt aagccgcctt
     7621 aacccggatt tactgtacgg caatgtgctg gcaagcggcg tcggcgtggg tacgctgacc
     7681 ctgttacaaa gcgacagcct cgacagttat cgggcaatcc ccgccagtgc gcaagattcc
     7741 acccggctgg agcacagcct ggcaacgctt gccgagcaac tgaaccagca attgcgtgag
     7801 cgtgacggcg aaagcaaaac tatcctcagc gcccatttgt cgctgattca ggatgatgaa
     7861 tttgcaggca atatccgtcg cctgatgaca gaacagcatc aggggctggg ggcggcgatc
     7921 atcagcaata tggagcaggt ttgcgccaaa ctttctgctt ctgccagcga ttatctgcgc
     7981 gaacgtgtta gcgacattcg cgatattagc gaacagttgc tgcatattac ctggccggaa
     8041 ctgaagccgc gcaacaatct ggtgctggaa aaaccgacta ttctggtggc cgaggattta
     8101 accccaagcc agtttttaag cctggattta aaaaatcttg cgggcatgat tctggagaaa
     8161 accgggcgca cctcgcatac cctgattctg gcccgcgcct cggcgatccc ggtactgagc
     8221 ggcctgccgc tggatgcgat tgcccgttat gccgggcaac ctgcagtgct tgacgcccag
     8281 tgcggcgtgc tggcgattaa cccgaatgac gcggtgagcg gttattatca ggtcgcgcag
     8341 acgctggcgg ataaacgcca aaaacaacag gcgcaggctg ccgcgcagct ggcttattcc
     8401 cgtgataaca agcgtattga tatcgcggcg aatattggca ccgctctgga agcgcctggc
     8461 gcgtttgcca acggcgcgga aggcgtcggg ctgttccgta ccgaaatgct ctatatggat
     8521 cgcgacagcg cgccggacga gcaggagcaa tttgaagcct accagcaggt gctactggcg
     8581 gcgggcgaca agccgattat cttccgcaca atggacatcg gcggcgataa aagcattcct
     8641 tatctgaata ttccccagga agagaacccg ttcctcggct atcgcgcggt acgtatttac
     8701 ccggaatttg ctggcctgtt ccgcactcaa ctgcgggcca ttttgcgcgc cgccagtttc
     8761 ggcaacgccc agttgatgat cccgatggtt cacagcctcg atcagatctt atgggtgaag
     8821 ggcgagatcc aaaaagcgat cgttgagctt aagcgcgatg gcctgcgtca tgcagagacg
     8881 attacgctgg gtatcatggt ggaagtgccg tcggtgtgct acatcatcga tcacttctgc
     8941 gatgaggtcg atttcttcag tatcggctcc aacgatatga cccagtatct gtatgcggtc
     9001 gatcgtaata acccgcgcgt atcgccgctt tataacccga ttacgccatc gttcctgcgc
     9061 atgttgcagc agattgttac cactgcgcat cagcggggca aatgggtagg catttgcggt
     9121 gaactgggcg gtgaaatccg ttatctgccg ctactgcttg ggctagacct ggatgagctg
     9181 agtatgagta gcccgcgcat tccggcggtg aaaagccagc tgcgtcaact ggatagcgag
     9241 gcgtgtcggg aactggcacg tcaggcgtgt gaatgtcgca gcgcgcagga aatcgaagcg
     9301 ttactcaccg cctttacgcc ggaagaagac gttcgcccat tgttggcgct ggagaatatc
     9361 ttcgttgatc aggcctttag caataaagag caggcgatcc agttcctgtg cggtaacctc
     9421 ggcgttatcg ggcgcactga acatccgttc gagctggaag aggatgtctg gcagcgggaa
     9481 gagattgtca ccaccggcgt tggttttggc gtggcgatcc cgcacaccaa atctcagtgg
     9541 atccgtcatt ccagtatcag cattgcccgg ctggtgaagc cggttgactg gcagtcagag
     9601 atgggcgaag tcgaactggt aatcatgctg acgctgggcg ctaacgaagg gatgaatcat
     9661 gtgaaagtct tctcgcagct ggcgcgtaaa ctggtgaata aaaacttccg ccagtcgttg
     9721 tttgccgcgc aagatgcaca aagtatcctg acgctgctgg aaacagaatt aaccttctga
     9781 cgttagccct gaaaacgggc gctgtactct cccggcgtca ggccaaactg acgccggaag
     9841 acgcgacaaa aatagtcgct gtccggaaag ccgcaacgct gcgccacttc atgaatcgac
     9901 agatgatatt tctgcaaaat catcctcgcc ttcgccattc gcacccaacg cacatattcg
     9961 ataaaacgca ttgttccatg ctgcgcaaac agttttgaca gatgattggg cgtgatatta
    10021 aaaaactgcg ccacgttctc acgggtgagc ggctgggcat aattatcctg aacccagtta
    10081 caaatgctgt gatagagaaa atcgccgcgc ggttgcgaat tacccggcgg catattcacc
    10141 acgttgcggc aaagatgcag caaacttaac accagcggct gaatgatatt ttgctccagc
    10201 ggcgaacggc taagatgggt gagggcggta agcatcgctt caccttcacc gcgttgcagg
    10261 ctgggaagct ggatctggcg agcaggatgc aacaacgacg tggtgcggct atcgtaaaac
    10321 gataacccaa gccaggtcgg agcgaacacc aggcttaaca gcatcaccgg tttgttgttg
    10381 accggtaaat tagccgcgcg cgccggaata aacagcattt ctccttccac caaacgttgc
    10441 tgctttgctt ccagcagatt gccgtattcg ccgcgcagca cgatatccag acggggaaaa
    10501 tcaatacata aactgcccac cggcaacgag gcaggctggc tggcaaacca gacgcgcccc
    10561 agtagttgcg ggttaagcac caggccgctg aacagatcgg cgaaaaattg ttgatcggcg
    10621 ggcaagcctg gtgctttcat gaaagttccc gattaaagat gcattaactg gcgaaattct
    10681 tttactttgc tgcggctgac tggcacttca aaatccagat ctttcagtcg cagaatatag
    10741 gtgttattaa accacggttc gatctcgcgg attttgttca gattgacaca aaatgagcga
    10801 tggcagcgga aaaaatgcga cggcggcagt ttgctgcaaa attcggtaat gttcatcggc
    10861 attacgtagg attcacgccg cgtatagaca aaggtcattt tctcgtgcgc ttcggcgtaa
    10921 tagatatcgt taattggcgt gacgattatt cgctcatctt tcaccagatt aatggtgtca
    10981 ttctcacgcg ttaccgtcgc ggcaggcgtg ctgctggtct gctgttgttg ccaggccgct
    11041 tccagttttt gcagcatccc ggtaatgcgt gactcctgat acggtttgag aatgtagtca
    11101 aacgcctcca gttcaaacgc ttctaccgca tgttctttcc acgcggtgat gaacacaata
    11161 aacggtttat gggcgaactg gctgatgttt tgcgccagca acacaccatc cagcgacgga
    11221 atattgatat ccagaaaaat ggcgtcgacg cggttatgct gcaaaaactt caacacgtcc
    11281 agaccgtcgt caaaggtgcc gacaatctcc atctggctgt gctctttaat tagccagctc
    11341 agttcctgtt gtgccaggaa ttcgtcttca acaatgatga ctttcacaat atcactccgg
    11401 ctcaaagcaa taacgtagcc tgtgaggcga ctggggtgcg ttggttagga atgtaaaacg
    11461 caatttccgt tcctggttcc aggcggcgga tatgcagccc ctcgccatac aataacttca
    11521 cgcgatgatg gacattcagc aggccaattt tattgcccgg catttcattc gcttcgaccc
    11581 gctcaatcac ctttggatcg atgccgtgcc cggtatcccg cacggcaatg cgtacccgat
    11641 tgccgcactc tgcaacgctg atggtgacaa cgcctttacc tttgcaaggc tggataccgt
    11701 ggacaatggc attctccacc aacggctgga tcagcaggct gggaatgcag caattcacct
    11761 cttcatcaat atcatagata accgtgagct tgtcgccaaa gcgggcctgc tcaatggcaa
    11821 tatagtcttt aatttgatac agctcttttt tgatatcgat ttgctcatcg tcttttaatt
    11881 caatgttata gcgcagataa cgcgacagat taaagatcag ctggcgagcg gtatccggat
    11941 tcagacggat cgacgatgaa atagcgttca gagcgttaaa cagaaaatgg ggattaattt
    12001 tgctttgcag ggcgcgaagc tctgccttat ttgccatttc acgtagctgc tcggcgcgtg
    12061 aaacctccag ttgcgtggag atgatttgcg acagaccaac cgccatttcc tgtaatgacg
    12121 aggttatctg atgcgcgtgg cagtagtaaa ttttcagcgt tccggtcaca acgcctttct
    12181 cccacaatgg gatcaccagc atggaatgaa tctctggtgt gcggtgggct tcatcattgt
    12241 ttttaatgat gatttttccg taattcatcg cctgacgggt agtcgggcta atgaagtcat
    12301 cgccattctg atagttatgt tcacccacac caacataggc cagcacatga tcggtattgg
    12361 taatcgccac cgcatcggcg tgaatgtcat cgcgaataat ttcacaaact ttgcgtaatg
    12421 actcgctatt gacatggcga aacagaggta gcgttttgtt ggcgatatcc agcgccaact
    12481 tggcctgccg cgcggcgctg gcctcttttt cgccctcaac gctttgcacc agaagcacaa
    12541 taaagccgat acagacgctg ccgaggatca ttggaatccc gattttagag acgatatcga
    12601 tccccaacgc ggtagttggt gcccagacaa tcaccagaat catggtcaac gtttcacaca
    12661 acatgccgcc gagaataccg acgcgccagc gctgtgcttt ggggattttc aggttgatcc
    12721 agcccgatat acaaccagcc agaatgctgg tgataaagca ggggatcgcc gtcacgccac
    12781 cgatatcaat taaataacga tgaatacccg caatcacgcc ggtaatgatg cccacccacg
    12841 ggccaaacag aatcccgccg gacatcaccg cgataatacg tacgttcacc agcgagcctt
    12901 ctacgggaac gccggaccag gtgctgaaga gggcgaacag cgagaaaatg gcggtaacgg
    12961 cgagcaactc tttcggtgag tgtgccgact tgtgcaacag ttcgcgaaac aggcggatac
    13021 ggatgagaaa gaacaggcag ataagcatta acgctgcccg atcgaagacc gccagcagca
    13081 tgttgaatat ttcgtgcacg ggtgactctg aataaggatt aaacctctat gataggtaac
    13141 ctgaaggctg atgaccagca ggccgttttt gaggaaaaac cacaatttta aaaggggatt
    13201 gatagataaa tggtgt
//
LOCUS       DAZKFU020000127        13093 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZKFU020000127 DAZKFU020000000
VERSION     DAZKFU020000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.128"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            263..4723
                     /gene="gltB"
                     /locus_tag="ACHOQQ_RS14735"
                     /old_locus_tag="pgaptmp_002947"
     CDS             263..4723
                     /gene="gltB"
                     /locus_tag="ACHOQQ_RS14735"
                     /old_locus_tag="pgaptmp_002947"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGU4471166.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIIETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSAFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4736..6154
                     /gene="gltD"
                     /locus_tag="ACHOQQ_RS14740"
                     /old_locus_tag="pgaptmp_002948"
     CDS             4736..6154
                     /gene="gltD"
                     /locus_tag="ACHOQQ_RS14740"
                     /old_locus_tag="pgaptmp_002948"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGU4471167.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6338..7465
                     /gene="yhcG"
                     /locus_tag="ACHOQQ_RS14745"
                     /old_locus_tag="pgaptmp_002949"
     CDS             6338..7465
                     /gene="yhcG"
                     /locus_tag="ACHOQQ_RS14745"
                     /old_locus_tag="pgaptmp_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HGU4471168.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSLYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGVGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRLR"
     gene            complement(7525..7989)
                     /gene="nanQ"
                     /locus_tag="ACHOQQ_RS14750"
                     /old_locus_tag="pgaptmp_002950"
     CDS             complement(7525..7989)
                     /gene="nanQ"
                     /locus_tag="ACHOQQ_RS14750"
                     /old_locus_tag="pgaptmp_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGU4471169.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(7986..8861)
                     /gene="nanK"
                     /locus_tag="ACHOQQ_RS14755"
                     /old_locus_tag="pgaptmp_002951"
     CDS             complement(7986..8861)
                     /gene="nanK"
                     /locus_tag="ACHOQQ_RS14755"
                     /old_locus_tag="pgaptmp_002951"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU4471170.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8858..9547)
                     /gene="nanE"
                     /locus_tag="ACHOQQ_RS14760"
                     /old_locus_tag="pgaptmp_002952"
     CDS             complement(8858..9547)
                     /gene="nanE"
                     /locus_tag="ACHOQQ_RS14760"
                     /old_locus_tag="pgaptmp_002952"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGU4471171.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVEMLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9595..11085)
                     /gene="nanT"
                     /locus_tag="ACHOQQ_RS14765"
                     /old_locus_tag="pgaptmp_002953"
     CDS             complement(9595..11085)
                     /gene="nanT"
                     /locus_tag="ACHOQQ_RS14765"
                     /old_locus_tag="pgaptmp_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGU4471172.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(11194..12087)
                     /gene="nanA"
                     /locus_tag="ACHOQQ_RS14770"
                     /old_locus_tag="pgaptmp_002954"
     CDS             complement(11194..12087)
                     /gene="nanA"
                     /locus_tag="ACHOQQ_RS14770"
                     /old_locus_tag="pgaptmp_002954"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4471173.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12209..13000)
                     /gene="nanR"
                     /locus_tag="ACHOQQ_RS14775"
                     /old_locus_tag="pgaptmp_002955"
     CDS             complement(12209..13000)
                     /gene="nanR"
                     /locus_tag="ACHOQQ_RS14775"
                     /old_locus_tag="pgaptmp_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU4471174.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         3088 a         3617 c         3541 g         2847 t
ORIGIN      
        1 ataagcttgc catttgacct gtatcagctt tcccgataag ttggaaatcc gctggaagct
       61 ttctggatga gcagcctgct cattatattt atgcagtaat tgagatcccc tcttcaccgt
      121 attaatcgat gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa
      181 accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt tcggtagtgg ggttcccgca
      241 gagcctgggg gaggttcacg atatgttgta cgataaatcc cttgagaggg ataactgtgg
      301 tttcggcctg atcgcccaca tagaaggcga acctagccac aaggtagtgc gtactgcaat
      361 acacgcactg gcccgcatgc agcaccgtgg cgcgattctc gccgatggta aaaccggtga
      421 cggttgcggc ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg
      481 cggctggcgt ttagcaaaaa actacgctgt cgggatgctc ttcctgaata aagatcctga
      541 actcgctgct gccgcacgcc gcatcgttga agaagagctg caacgcgaaa cgttgtcgat
      601 tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc
      661 tctgccacgc attgagcaaa tttttgtgaa cgccccggca ggctggcgtc cgcgtgatat
      721 ggaacgccgt ctgtttatcg cccgccgccg catcgaaaag cgtctcgaag ccgacaaaga
      781 cttctacgtc tgtagcctgt cgaatctggt caatatttat aaaggtctgt gtatgccggc
      841 ggatctgccg cgcttttacc tggatcttgc ggacctgcgc ctggaatcgg ccatctgcct
      901 gttccaccag cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc agccgttccg
      961 ctatctggcg cataacggtg aaatcaacac catcaccggt aaccgccaat gggcgcgtgc
     1021 gcgtacctat aaattccaga caccgcttat ccctgacctg cacgacgccg caccgttcgt
     1081 caacgaaacc ggctctgact ccagttcgat ggataacatg ctggaactgc tgctggcagg
     1141 cgggatggat atcatccgcg ccatgcgtct attagtacca cccgcctggc agaacaaccc
     1201 ggatatggac ccggaactgc gtgccttctt tgactttaac tccatgcata tggagccgtg
     1261 ggatggcccg gcgggcatcg tgatgtctga cggtcgtttt gctgcctgta acctcgaccg
     1321 taacggtctg cgtccggcgc gctacgtcat caccaaagat aagctcatca cctgcgcctc
     1381 tgaagtcggt atctgggatt accagcctga cgaagtggtc gaaaaaggcc gcgtcgggcc
     1441 aggcgaactg atggtgatcg acactcgcag tgggcgtatt ctgcactcgg cagaaaccga
     1501 tgacgatctg aaaagccgcc acccttataa agagtggatg gagaaaaacg tccgccgact
     1561 ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg acgacgacac
     1621 gcttgccagc taccagaaac agtttaacta cagcgcggaa gagctggact ccgtaattcg
     1681 cgtactgggc gaaaacggtc aggaagcggt cggttcgatg ggcgatgata ccccattcgc
     1741 cgtgctctcc agtcagccgc gcattattta cgactacttc cgccagcagt ttgcccaggt
     1801 gactaacccg ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg ccaccagcat
     1861 tggtcgtgaa atgaacgtct tttgcgaagc agagggccag gcgcaccgtt taagctttaa
     1921 atcgccgatt ctgctctact ccgatttcaa acagctcacg acgatgaaag aggagcacta
     1981 ccgcgcagat acgctggata tcacctttga cgtcaccaaa accacgctcg aagcgacggt
     2041 caaagagctg tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgt tgctggtgct
     2101 ctccgaccgg aatatcgcta aagatcgcct gccggttccg gctccgatgg cggttggcgc
     2161 gatccagacc cgtctggtcg atcaaagcct gcgctgcgat gccaacatca tcatcgaaac
     2221 cgccagcgcc cgcgatccac accatttcgc cgtgttgctg ggcttcggtg cgacggctat
     2281 ttatccgtac cttgcctatg aaacgctggg ccgcctggta gacacccacg cgattgccaa
     2341 agattatcgt accgtgatgc tcaactaccg taacggcatc aacaaaggct tgtacaaaat
     2401 catgtccaaa atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc
     2461 ggtcggtctg cacgatgatg tagtgggcct gtgcttccag ggggcggtca gccgcattgg
     2521 gggggcgagc tttgaagact tccagcagga tctgctgaat ctgtcgaaac gtgcctggct
     2581 ggcgcgcaag cccatcagcc agggcggcct gctgaaatac gtccacggcg gcgaatacca
     2641 cgcctacaac ccggacgtgg tgcgcacgct gcaacaggcg gtacagagcg gcgagtatag
     2701 cgactatcag gaatacgcga agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct
     2761 gctggcaatt acgccgggtg aaaacgcggt caacattgcg gatgttgaac cggcaagcga
     2821 actgtttaaa cgcttcgata ccgctgcgat gtctatcggc gcgttaagcc cggaagccca
     2881 cgaggcactg gcggaagcga tgaacagcat cggcggtaac tctaactccg gtgaaggtgg
     2941 tgaagacccg gcgcgctacg gcaccaacaa agtgtcgcgc atcaagcagg tggcttccgg
     3001 tcgctttggg gttactccgg cgtatctggt caatgccgac gtcattcaga ttaaagtcgc
     3061 ccagggcgcg aagccgggcg aaggcggtca gttgccgggc gacaaagtca ctccttacat
     3121 tgccaaactg cgctattcgg tgccgggcgt gacgctgatc tccccgccgc cgcaccacga
     3181 tatctactct atcgaagact tagcgcagct cattttcgac ctcaagcagg ttaacccgaa
     3241 agcgatgatc tccgtgaagc tggtttccga accgggcgtt ggtaccatcg ccaccggtgt
     3301 ggcgaaagct tatgccgatt taatcaccat cgcaggctat gacggcggca ccggcgcaag
     3361 cccgctgtcg tcagtgaaat acgcaggttg cccgtgggag ctggggcttg ttgaaaccca
     3421 gcaggcgctg gttgccaatg gtctgcgtca taagatccgt ttgcaggtcg atggcggcct
     3481 gaaaacgggt gtcgatatca tcaaagcggc gattctcggc gcggaaagct tcggcttcgg
     3541 cactggccca atggtggcgc tcggttgtaa atatctgcgt atttgccacc tgaacaactg
     3601 cgccacgggt gtagcaactc aggatgacaa actgcgtaag aatcactatc acggcctgcc
     3661 gttcaaggtg acgaattact ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca
     3721 gcttggcgta acacgtctgg tggatctgat tggtcgcacc gacctgctga aagagctgga
     3781 cggtttcacc gccaaacagc agaaactggc gctgtcgaag ctgctggaga ccgccgaacc
     3841 gcatccaggt aaggcactct actgcaccga aaccaacccg ccgtttgata acggcctgct
     3901 gaacgcgcag ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga gcaaaacttt
     3961 ctggttcgat atccgcaaca ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc
     4021 ccagacgcac ggcgatcagg ggctggcagc cgatcctatc aaagcgtact tcaacggcac
     4081 cgcaggccag agtttcggtg tgtggaacgc gggcggcgtg gaactgtacc tgaccgggga
     4141 tgccaacgac tatgtcggta aaggcatggc gggcggctta atcgccattc gtcctccggt
     4201 tggttccgcc ttccgcagcc atgaagcaag tattatcggc aacacctgcc tgtatggcgc
     4261 gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg tgcgtaactc
     4321 cggtgcaatc accgtggtag aaggcattgg cgacaacggc tgtgaatata tgacgggcgg
     4381 tatcgtctgt attctgggta aaaccggcgt taacttcggt gcgggtatga ccggcggctt
     4441 cgcttacgtt ctcgatgaaa gcggcgattt ccgcaaacgc gttaacccgg aactggtgga
     4501 agtattaagc gttgacgatt tggcgatcca tgaagagcat ctgcgtggtc ttatcaccga
     4561 gcatgtgcag cataccggtt cccagcgtgg tgaagagatt ctggcgaact ggtcagcctt
     4621 cgccactaaa tttgcgctgg ttaaaccgaa gtccagtgat gtcaaagcac tgctgggtca
     4681 ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag taaggggtag ctacaatgag
     4741 tcagaatgtt tatcaattta tcgacctgca gcgcgttgat ccgcctaaga aaccgctgaa
     4801 gatccgcaaa attgagtttg ttgaaattta cgagccgttt tccgaaggcc aggccaaagc
     4861 gcaggctgac cgctgcctgt cgtgcggcaa cccatactgc gagtggaaat gcccggtaca
     4921 caactacatc ccgaactggc ttaagctcgc caacgagggg cgtatttttg aagcggcaga
     4981 actgtcgcat cagaccaaca ctctgccgga agtatgcgga cgtgtctgcc cgcaagaccg
     5041 tctgtgcgaa ggttcctgca ccctgaacga tgagtttggc gcggtgacca tcggcaacat
     5101 tgagcgttat atcaatgata aagcgttcga aatgggctgg cgtccggata tgtccggcgt
     5161 gaaacagacc ggtaaaaaag tggcgattat cggcgcaggt ccggcaggtc tggcgtgtgc
     5221 agatgtcctg acgcgcaacg gcgtacaagc ggtcgtcttt gaccgtcacc cggaaattgg
     5281 cggcttgctg accttcggta ttccggcttt caaactggaa aaagaggtaa tgacgcgccg
     5341 ccgtgaaatc ttcaccggca tgggtattga attcaaactc aataccgaag tgggccgcga
     5401 cgtacagctg gacgatctgc tgagtgatta cgatgccgtg ttccttggcg tcgggactta
     5461 tcagtcaatg cgcggcgggc tggaaaacga agacgctgat ggcgtgtacg cagcgctgcc
     5521 gttcctcatc gccaacacca aacagttaat gggctttggt gaaaccaacg acgaaccgtt
     5581 cgtcagcatg gaaggtaaac gcgtggtagt acttggcggt ggcgataccg cgatggactg
     5641 cgtgcgcacg tctgtacgcc agggagcgaa gcacgtaacc tgtgcctatc gtcgtgatga
     5701 agagaacatg ccaggttccc gccgcgaagt gaaaaacgcg cgggaagaag gtgttgagtt
     5761 caaattcaac gtccagccgc tgggtattga agtgaacggc aacggcaaag tcagcggcgt
     5821 aaaaatggtg cgtaccgaaa tgggcgaacc ggacgccaaa ggccgtcgcc gcgcggagat
     5881 cgtggctggt tccgaacata tcgttccggc agatgcggtg atcatggcgt ttggtttccg
     5941 tccacacagc atggaatggc tggcaaaaca cagcgtcgag ctggattcgc aaggccgcat
     6001 catcgccccg gaaggcagcg acaacgcttt ccagaccagc aacccgaaaa tctttgctgg
     6061 cggcgatatc gtccgtggtt ccgatctggt cgttaccgct attgccgaag gtcgtaaggc
     6121 ggcagacggc attatgaact ggctggaagt ttaagctctg tcccagcgcc gcctcctcag
     6181 aaaagagtcg gcgctgaaat tctcctttcg ttctggaaag cgcctcgcat aatctgaaga
     6241 aaaccaatgt aatacgcaca tctggattgt taccggcaga tgcccggcgt atagctgatt
     6301 ccattattca acactgcgcc atataaaggg aaagataatg gaatcgctct cagagagaac
     6361 ctcaacaggc taccagcaaa tccacgacgg cattattcat ctggtcgata gcgcccggac
     6421 ggaaacggta cgtagcgtta acgcgctgat gaccgcgaca tactgggaaa ttggccgacg
     6481 aattgtcgaa tttgaacaag gtggcgaggc cagggctgcg tatggtgcgc agctaatcaa
     6541 gcgactatca aaggatttaa gtctaaggta taagcgaggg ttctctgcaa aaaacttacg
     6601 ccaaatgagg cttttttacc tcttttttca acatgttgaa attcgccaga cagtgtctgg
     6661 cgaattaaca ccattgggaa ttccgcagac accatctgca gaattcccat cggcaaagat
     6721 ttggcagacg ctgtctgcca aatctttccc cctgccctgg tccacctacg tccgtttact
     6781 ttccgttaaa aacgctgacg cccgcagcct ttatgaaaaa gagacgctcc gctgtggctg
     6841 gtctgttcgc cagctagaac ggcaaattgc gacccaattt tatgagcgaa cactgctgtc
     6901 acatgacaaa tcagccatgc tgcaacaaca cgctcctgcc gagacgcata ttcttccgca
     6961 acaggcgata cgcgatccct ttgtgctcga atttctggaa ttgaaagatg aatactcaga
     7021 atccgatttt gaggaggcgc tgatcaacca cctgatggat ttcatgctgg aacttgggga
     7081 tgattttgcc tttgttggtc ggcagcgaag gttacgcatt gatgacaact ggtttcgtgt
     7141 cgatctgctg tttttccacc gccgtttacg ctgcctgcta atcgtcgatc taaaagtggg
     7201 caaattcagc tatagcgatg ccggacagat gaatatgtat ctcaactacg ccaaagagca
     7261 ctggacgcta ccggatgaaa atccgcccat cggtctggtt ctctgtgcag agaaaggagt
     7321 cggagaagcg cattatgcac tggctggttt gcctaacacc gttctggcga gtgaatataa
     7381 gatgcaacta cctgatgaaa aacgactcgc ggatgaactc gttcggacac aggcggtgct
     7441 agaagaaggc tatagactcc gttaatttca gcggtttcga attctgcaga cactgtctgc
     7501 agaatcttta aaacgaacta agttttaagc cattaaatca gccttaacct tcaccacaac
     7561 ctttttaatc tcaccaggtt cgccgacaac gcatcctggt ttatgcggtt ctcccggcat
     7621 aaacacggcg aacattcccg gttttaagtt gatggtttgc tcgttttcaa tggcgctgca
     7681 aagctggtaa tcatcttcat ggtggaactc ttcacactgg cgcgcagtgc ctgccgtgcc
     7741 aaacagaatc cgttcctcac cttttaataa cagctggata tcaatgtatt gctcgtgcaa
     7801 ttccgctttt ttctcgacgg gcgattgagt gttaaacgtc atgacattca taaaaatatt
     7861 gtcgccctgt aattcgtaac gacccggcgc tttttcttgc ggtctggcag ctaatgccag
     7921 cgttaacgcg tcctgtaacg caggatgtaa cccagcagac ggtaatgact gtacttcacc
     7981 catcatcata atttttctcc ctgggccaac agcgcagccc caagtaaacc tgcatcatgg
     8041 cggtagtgcg ccgccagtaa atcaacatga aatgccgctg gctcctgcgc cagatacgtt
     8101 tccaccagcg ccagataccc ttctgccaga ccaacgctgc caccgaccac cacgcactga
     8161 caatcagttg tggctttaat atcagcgatc agccttgcca gcacatgtgc ggagcggtga
     8221 atcagctgct gcgcctgctc gtcgccctgt ccggcgcgca tgaaaatagt tttcgcatcc
     8281 gcgccagcca actccccctg cgctgccgct gcaataccgc gaccagaagc aatcgcttcc
     8341 acgcaacctg tgcgtccaca gccgcagact gggccgtgtg gatcggcaag cgtatgcccg
     8401 atgtgccccg ccagaccgcc agggccggta agcagtttgc cgccgctcac tacaccgccg
     8461 ccgacgccag tggaaacggt gataaacacc atctcggtta catcgccttc cagcgcctga
     8521 tactctgccc atgctgcggc ctgcgcgtcg ttaatggcaa tggtcggcaa attggtaagt
     8581 tgttccagcg ttttgactaa cggaaagtgt aacaatccac caagattatg cggattcagc
     8641 gccagcaagc tgccgtcgcg aataatcccg gttgaagcga tggcaacccg ctgcgcatga
     8701 gcttgcaacg gagagactaa tgcggataag gcatcacgca gtgcctgcgg agtctggctg
     8761 gcaggcgtag gaagttctcg gcgatcgcgg atctgcccgt cagcgccaat cagcgcggcg
     8821 gcaagtttag taccgccgat atcaatcgcc agcgtggtca tagcaccgcc tttttcatcg
     8881 ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat ggcagaaccg accgtcaccg
     8941 cccacgcgcc gtggcgcatc gcatcagccg cctgagcagg cgtgttgtaa cgcccttcgg
     9001 caatcacccg acatccggcg tcgctcaacc ttttcaccag cgtcagatcc ggctcttctg
     9061 gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat aatttcggct cccagctttt
     9121 ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat cgccagtaaa ccgtgatggt
     9181 ggatacgtgc cagcagcatt tcaacaggca ccggacgcgg gcggtcggtg ccgtcaatgg
     9241 cgataatgtc cgcgcccgcc tgcgccagcg catcgacatc ttcaatatag gccgtgatgc
     9301 gtaccggaga atcctccaga tcgcgtttca caattccaat aatcggcacg ctcaccaccg
     9361 cacgcgtggc ttgcagattt gccacacctt cgatgcgaat ggcaaccgcg cccgcctgct
     9421 ctgccgctaa tgccatggct gcgacgattt cgggtttatc gagcgggctg tcaggaaccg
     9481 gctggcagga gacaatcagg ccaccgttag cagcgatttt ttgatccagt tgtgcaagta
     9541 acgacataca tcttccctta gcgaaaggcc cggtacatag accgggcaac aggattaact
     9601 tttggttttg actaaatcgt ttttggcgct gccaaatggc acggcaccgc taaatggttt
     9661 gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc agccaacgct gaacgcggga
     9721 aggcatatcc agaccaatca gcaggatcac cacaaatgtc agactgaacg agagcgatgc
     9781 cagcgcagta cccagatcca gacgttgagc gatcaacgcg ccgatgattg gggccagtgc
     9841 gccacccaat gcgccaacgt tataggtaaa gcccaggccc gctgcacgct ggtcggtatc
     9901 gaaataaccg ccaatcagtt tcggtaagat cccggcgatc ccttgtccaa gcatttgctg
     9961 gaagaacagt aacagaccga gcacccagac gtttgcaccg ccaatcgcaa ataccggaat
    10021 aatcagcagc tgcgaggcca acagactaca aacgtacgct ttgcgggttc ccagccagtc
    10081 accgaggaag ccaccgacgc agcaccccac cgccgcgcca aagccactaa agaacagcac
    10141 attggcgaca gtatgcgggt tataagccag atcggttttc agatacgttg gcagcagcgc
    10201 ctgaatcggc catgagtaga ggaaagcaaa caacacgacc accatcagca ttacgccagt
    10261 tggccagcgt ttgcccgtac tctgcaccat aaagctgata aagattgcgg cgcataacag
    10321 cccaagaaca gcgacgatcg cggcattttg caggttaccg gcgaagcaga accacagcgc
    10381 agtagccgcc gccagtgtca ttacgatatt ggcaatgcga tgttcaccac ggtagagaat
    10441 atccaccatt gtgcgtactg gtgctttacc tgcgtgtttc tctttccagt cttccgcttc
    10501 cgggatgttt ttacgcagcc agagagcaaa gataattggc aaaatgccga taaagaacag
    10561 cgcacgccag ccccagaccg gaaccaccag gctgtagacc tgagcggcaa cgacggcccc
    10621 cacagagaag cctgaaatca gaaagccact ggctttgtta cgcagatgtt ttggccagct
    10681 ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc atccccatgc cgatgaccag
    10741 acgagcgata aacatggtga tgtagcctgg cgcaaagccg caggccagcg tcccggccga
    10801 gaagagaacg atgctggtga ccattgccag acgacgcccg tagcggtcac ccatagcgcc
    10861 gagcatcagg ccgccgaacc agcgagagat aaaggctgcg gagatcagac ttgccgcctg
    10921 caccgtcgtc agcccgaatt caccttgtac ttcggtgagt accagggcga ttaaaacgaa
    10981 atcaaaaccg tcaagcagat atcccaacca ggcagcggaa aatgcccgcc attgtgcacg
    11041 attgagatgg cgataccacg ggatattctg ggttgtagta ctcattgtga gtctcccgcg
    11101 gtgggcgatg cccacacgct ttggtatgaa aattgtaggg tacagatgcg tttatttccc
    11161 ctcacccggt aggggcgagc gaggggaaac aactcacccg cgctcttgca tcaactgctg
    11221 ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggaccaaacg gtttgcggca
    11281 cagcggcaca gacacgacat ccatataatg cagaacagtt ttcaggccgc ggaatacgcc
    11341 cgttttgatc agtaaatcaa tgactttatt gcactcagtt tgcagtttct gcgcggtctg
    11401 gatatcgcct tctttcagcg ccttcacgat cccctgatag cgccagccca tgatgttgta
    11461 agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga tttcgtcgta
    11521 accgttatag agcaccagat caggatgttc acgacggatc tgttccatct gatagagatc
    11581 gccagaagtc tgtttcagcg caccgacgcc aggcaacgta acaagtgtgt tgatctgatc
    11641 cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg gcaacccatc
    11701 cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga aaggatagta
    11761 gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg ccgcaagttg
    11821 ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct tacctttcgc
    11881 ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta caaaggcttc
    11941 gccggtcgaa ccacccacgt ataaaccgtc aatgccctgc tgaatattga actgaaccag
    12001 gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca ggagtgcagc
    12061 cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg tctgataaac
    12121 gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac agaaagctga
    12181 taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc tgaccgaagg
    12241 cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg gcttcgtcag
    12301 gatcatgacg ccggatcgcg tcaacgatcg caatatgctg ttgatagcta acgttgttat
    12361 gctcgtgcag agcctgatca gccacggttg gacgtgcggc gatcagccag tcgagcaggg
    12421 caacgtggat cgccatgaag attgggttac ctgggatctc cgccagcacg cggtggaaat
    12481 caacgtctga gcgaatgaat gccgcgttgt tatccagcga ctgactgttg atttccagtg
    12541 cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag cgcaccaggc
    12601 tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga tgagaaagga
    12661 aatctttcgc catgccggaa agctcaccga tgatggtgtc cgcagaagga cgcgagacgc
    12721 gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct gccagcgctt
    12781 cacgcaccga aggacgcccg acgttaaaga acgccatcag ttcgcgttca gacggtaatt
    12841 gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct tccaccattt
    12901 cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt gcaggtgaag
    12961 aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt cagtgtgatt
    13021 aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc agggttttct
    13081 ctttgtgcct cat
//
LOCUS       DAZKFU020000128        13062 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZKFU020000128 DAZKFU020000000
VERSION     DAZKFU020000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.129"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(35..400)
                     /gene="yhaH"
                     /locus_tag="ACHOQQ_RS14780"
                     /old_locus_tag="pgaptmp_002956"
     CDS             complement(35..400)
                     /gene="yhaH"
                     /locus_tag="ACHOQQ_RS14780"
                     /old_locus_tag="pgaptmp_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4471175.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(693..1679)
                     /gene="yqjG"
                     /locus_tag="ACHOQQ_RS14785"
                     /old_locus_tag="pgaptmp_002957"
     CDS             complement(693..1679)
                     /gene="yqjG"
                     /locus_tag="ACHOQQ_RS14785"
                     /old_locus_tag="pgaptmp_002957"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGU4471176.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1749..2141)
                     /gene="yqjF"
                     /locus_tag="ACHOQQ_RS14790"
                     /old_locus_tag="pgaptmp_002958"
     CDS             complement(1749..2141)
                     /gene="yqjF"
                     /locus_tag="ACHOQQ_RS14790"
                     /old_locus_tag="pgaptmp_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4471177.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2327..2626)
                     /gene="yqjK"
                     /locus_tag="ACHOQQ_RS14795"
                     /old_locus_tag="pgaptmp_002959"
     CDS             complement(2327..2626)
                     /gene="yqjK"
                     /locus_tag="ACHOQQ_RS14795"
                     /old_locus_tag="pgaptmp_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGU4471178.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2616..3020)
                     /gene="yqjE"
                     /locus_tag="ACHOQQ_RS14800"
                     /old_locus_tag="pgaptmp_002960"
     CDS             complement(2616..3020)
                     /gene="yqjE"
                     /locus_tag="ACHOQQ_RS14800"
                     /old_locus_tag="pgaptmp_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4471179.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3023..3328)
                     /gene="yqjD"
                     /locus_tag="ACHOQQ_RS14805"
                     /old_locus_tag="pgaptmp_002961"
     CDS             complement(3023..3328)
                     /gene="yqjD"
                     /locus_tag="ACHOQQ_RS14805"
                     /old_locus_tag="pgaptmp_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGU4471180.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3366..3734)
                     /gene="yqjC"
                     /locus_tag="ACHOQQ_RS14810"
                     /old_locus_tag="pgaptmp_002962"
     CDS             complement(3366..3734)
                     /gene="yqjC"
                     /locus_tag="ACHOQQ_RS14810"
                     /old_locus_tag="pgaptmp_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGU4471181.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3881..4264)
                     /gene="mzrA"
                     /locus_tag="ACHOQQ_RS14815"
                     /old_locus_tag="pgaptmp_002963"
     CDS             complement(3881..4264)
                     /gene="mzrA"
                     /locus_tag="ACHOQQ_RS14815"
                     /old_locus_tag="pgaptmp_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGU4471182.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4268..4930)
                     /gene="yqjA"
                     /locus_tag="ACHOQQ_RS14820"
                     /old_locus_tag="pgaptmp_002964"
     CDS             complement(4268..4930)
                     /gene="yqjA"
                     /locus_tag="ACHOQQ_RS14820"
                     /old_locus_tag="pgaptmp_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGU4471183.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4952..5035)
                     /gene="yqiM"
                     /locus_tag="ACHOQQ_RS14825"
                     /old_locus_tag="pgaptmp_002965"
     CDS             complement(4952..5035)
                     /gene="yqiM"
                     /locus_tag="ACHOQQ_RS14825"
                     /old_locus_tag="pgaptmp_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGU4471184.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5275..6051)
                     /gene="exuR"
                     /locus_tag="ACHOQQ_RS14830"
                     /old_locus_tag="pgaptmp_002966"
     CDS             complement(5275..6051)
                     /gene="exuR"
                     /locus_tag="ACHOQQ_RS14830"
                     /old_locus_tag="pgaptmp_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGU4471185.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQTADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6181..7479)
                     /gene="exuT"
                     /locus_tag="ACHOQQ_RS14835"
                     /old_locus_tag="pgaptmp_002967"
     CDS             complement(6181..7479)
                     /gene="exuT"
                     /locus_tag="ACHOQQ_RS14835"
                     /old_locus_tag="pgaptmp_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGU4471186.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7962..9374
                     /gene="uxaC"
                     /locus_tag="ACHOQQ_RS14840"
                     /old_locus_tag="pgaptmp_002968"
     CDS             7962..9374
                     /gene="uxaC"
                     /locus_tag="ACHOQQ_RS14840"
                     /old_locus_tag="pgaptmp_002968"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGU4471187.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9389..10876
                     /gene="uxaA"
                     /locus_tag="ACHOQQ_RS14845"
                     /old_locus_tag="pgaptmp_002969"
     CDS             9389..10876
                     /gene="uxaA"
                     /locus_tag="ACHOQQ_RS14845"
                     /old_locus_tag="pgaptmp_002969"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGU4471188.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQKRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10959..11510
                     /gene="ygjV"
                     /locus_tag="ACHOQQ_RS14850"
                     /old_locus_tag="pgaptmp_002970"
     CDS             10959..11510
                     /gene="ygjV"
                     /locus_tag="ACHOQQ_RS14850"
                     /old_locus_tag="pgaptmp_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGU4471189.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPSAVDERG"
     gene            complement(11514..12758)
                     /gene="sstT"
                     /locus_tag="ACHOQQ_RS14855"
                     /old_locus_tag="pgaptmp_002971"
     CDS             complement(11514..12758)
                     /gene="sstT"
                     /locus_tag="ACHOQQ_RS14855"
                     /old_locus_tag="pgaptmp_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGU4471190.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3309 a         3353 c         3351 g         3049 t
ORIGIN      
        1 cggatgagtc agacatccgg cgagcagatt tgttttaagg ttccagtttt ggatcaggtc
       61 caaagcggtt ttcgcctggc gtacccgcct ggcagttaaa gacgatgata ataagccagc
      121 cgataaacgg gattaagaat agcagtgccc accacgccga gcggtcggtg tcgtgcaggc
      181 ggcggaactg aactgcccac cacggtaaaa acaccagaat accgtagatt gtcgtcagga
      241 tgccttcgcc accagcgcgt tgccagccta acattttatc cagcagcccc agcacgaacg
      301 taaagatgat gttgaccaga ataaacatcc agtactcttt gcgccgcgca cgaccccgga
      361 aaccgacata attttttagt actttcagat accagtccat ttttgcctca attttgctca
      421 atcacttgtt tttaaagcag caaattaaga atagacgtga atgaaatggc agtaaatatt
      481 cgttcaagga attaataaag tgctgtgaag gcgcgctaag actgatgaca agcgcaaaat
      541 tgccagatgc gctacgctta tctagcctgc gcggtcattg caacatattg aatataaaag
      601 ttttgtaggt ttgataaggc gttcatgccg catccgacat gaacaacatg cacttagcta
      661 attatcagag gcacctcgta aggtgccttt ttttaaccga agcgaacatc tcgtccatgc
      721 ggttcatcga gatcctgcca cggaccaatt gaaataatcc ccgtagggtt gatggtctta
      781 tgactgcgga agtaatgatt acggatatga tcgaaattga ctgtttcggc aattcccggc
      841 atctggtaga tatcacgcag gaagccatac agattaaggt aatcgctgat gcggtgctta
      901 tcacacttga agtgggtcac atacactgga tcaaaacgga ccagcgtggt ccacaggcga
      961 atatcggctt cggttagctg gttgccggtc aggtaacgat gctgacctaa aatctgttcc
     1021 agtcgcgcca gcgattcaaa cactttctcc accgcttcat cgtaggcttg ctggctggtg
     1081 gcaaaaccag ctttatacac gccgttgtta acggtgtcat aaatccagcc gttaagttcg
     1141 tcaattttcg tttgcagggc tggtgggtag taatcacccg ctttcgcgcc cagcgcatca
     1201 aacgcggtat taaacatgcg gatgatttct gctgattcgt tgctgacgat ggtgtggttc
     1261 tttttgtccc acagcacggg aacagtaact cgtccgctgt agtgtggatc ggcgtggaga
     1321 taaagctgat acaaaaattc atgttgatag agcgtgtctc cggttgctcc cggaaaactg
     1381 tcgtcaaagg tccagccgtt ttccagcatc agcgggttca ctacggaaac ggaaataaac
     1441 ggttccagtc ctttgagttt gcgcatgatc agcgtgcggt gcgcccacgg gcaggcgagt
     1501 gaaacataga gatgataacg atctttctct gcgataaaac cgcctgtgcc agtggggcct
     1561 ggagcgccat cggcagtgag ccagttacga aatgcggaag ctgaacgttg aaatttaccg
     1621 ccggtagatt tggtatcgta ccaggtgtca tgccagacgc cgtcaatcag ttgacccatt
     1681 tttctctcct ccaatagcaa aaacgaggag cttcctcctc gttttttatc gttaagtata
     1741 gcgcgtgctt accacttttt attcagcagg cggtcgatgc tatacgcgcc cggaccggta
     1801 attgccagca gcaggaatcc gccagaaatt gtcaggtttt tcatgaacat cagcgagttg
     1861 acgccttcag caaagttgct gtgaaataaa aatgccgtca gcagcgtaaa gcccgcagta
     1921 aacagggctg tggtgcgagt caggaaaccg aacaggattg ccagaccacc accaaactca
     1981 agcagaatca ccagcggcag cataaaaccc gggacgccca tcgcttccat atattgttgg
     2041 gtacccgcgt aaccagtaat ttttccccag cctgcggtaa taaacagaat cggcattaaa
     2101 atgcgcgcta ccagtacacc aacatcttct aattttttca tcatactctc cactgcgcca
     2161 ctcaggctgc tgattgtttt tttgtgcaag ggcgcggtat tagcgtcgtt gctgtcgatg
     2221 gagagaatca taaacgtggt gaatgatgat tgttagcaag gaaaactgtc aaaaatcttc
     2281 aaaaaatttg atgggtaagg ccggaatggc tccggccaga gagaagttaa ccgcgaagct
     2341 gttgctgctt gagggtcgtt ttaaccagac gccaggcgct ccatacgcca aaaccgcgtc
     2401 tggcccagcg gaccagcata ttaggatggc gaatcgtcca gatcgccatc acgctactgc
     2461 caaccagcgc ccaggagcgc agacttagca gcatattcca gcgacgatcg taagcgcctg
     2521 ttgcctccag ccattcacga cgactggcag aaagatccag ccgttgttgc tggatctggc
     2581 taagcagttg cgccttacgt cgttcacgtt cgactttact gctcacggga ctcctcctcg
     2641 agcagctgcc gatcgtttgc cagctcatgg cgtgtatggc gcagcaaggt ggatttacgc
     2701 gatttacgta acgtccagat accgccaatc agtgccagta gcagcaacac cacggtggtg
     2761 gcaatcatcg cattcaggcg atattgcgga tcaaccgccc aaataattag caccatcagg
     2821 ctcataagac caaatgcagc gaaaagcatc gtcaggccca gcatcagtaa aagttgaaag
     2881 agattagctt tttcctcttc cagctccacc accgccagcc gcagacgtgt ttccaccatt
     2941 tcaaccatga tggaaacaat tcgctgcccg atgcccagaa cgcttttacc gggcccttgc
     3001 gcgtgatgag tgtccgccat aattaacgac gcgacagcag aacgccgagc actacaccga
     3061 ttgcagcgcc aatgcccacg cccgtccatg gattttcacg cacatactca tcggcacgcg
     3121 ccgccgcgac acgggtttgt ttggcgatgg catcaccggt ttcacccagg cgataacggc
     3181 tctgtttcag tgcctgctcc gctttgctac gaatcttact caactcttct ttcgacttct
     3241 cgccagatga gctaagcacc tcttccagcg tatcggaaag ggatttcaac tcagcacgca
     3301 gatgttccgt agtgtgttct ttcgacatag ttttctccag gtgagtaagt agtgactatt
     3361 gtgagttagt agtcgcgcgc ttccagcttt ttcagctctt cctgcgcttc tgccagtttc
     3421 cgttcgcgtt tggcaatctt atcggcatcg cctttttgct tcgcctcggc taaatcttgc
     3481 tggcgttccg ccacctcatc tttctgcttt gcgattttct tctgatgatc ggcacgcagc
     3541 tggctatctg aacagttggc ccggacttca ctcagggctt tattcagacc gtcaatacga
     3601 ttctggtttt ggtgtttttc ggcatagctg atctccttaa ggatattttg ctccttttcc
     3661 tgacacaggg tagtggcata actaccggca ctaagagcaa aaagagaaac agctaaagcg
     3721 atgcggtatt tcattcttga accttccatt gtgattcgga cctattacaa aggaacgatt
     3781 gtccagacaa cgaagcggta gtgttaacca ccgcctcatt aatcatagac agttacggtg
     3841 aaatcaccaa agtgagtgat ctgtttcgga taacaggaag ttatccgaag cgatgtgagt
     3901 tatcccgtag ccgggtcagc cactgcatag cctgactgtt attgtcctgc tgcgcaatga
     3961 tgtagccttg gggcaatgtt ctgtcgagga ccgcttttgc agcggcgctc tggtcgctgg
     4021 agtcaaaggt aattaacaga gtgtcgtttt tgggggtgat acttttgaaa ggaatgccat
     4081 gagcgtcaag gtgatgccag attgaaaaac cgtctggcat cgttgtgcct tgatgaacgg
     4141 cacgaatcgc cagcgtagac tcttgctggc gaaccgcgga ccaggccagc aacagcgtgc
     4201 cgaccagaag taaaacagcg ccggaccagg ctagctggcg aagcgacatg cgaggtattt
     4261 gcatccctta cccccgattt ccatatttct ttttccataa cacgaccaga gaacctgcca
     4321 ggccaaacac cagcagcacc accgggagca gcatcaggca tgacatcagc tggtcctcgt
     4381 actttaaaaa taccggcgtt ttgccgagca tgtaacccag agttgtcagg atcaataccc
     4441 acagcagacc gctcatccag ttgaaaaact gaaagcgcgc gttattcagc cctgataacc
     4501 cggcaatcgt cggcagcagt gttctgacaa acgcaataaa gcgaccaatt aacagcgccg
     4561 ataaaccgtg tttatgaaaa agatggtgtg cgcgttgatg ataatgcgcg ggtaaatgag
     4621 atagccagtt ttgtacggtg cgggtattgc ccagccatcg cccctgaata tagctgaccc
     4681 agcagccgag gctggcggca acggtcagca gcagaatcgt ttgcggatag cccatcgcgc
     4741 ctttcgcaat caacacgccg accaatacca gtaaactgtc gcccggtaaa aaggccgccg
     4801 gaagcaagcc gttttcaagg aacaaaatta caaacaagac aaaatacaac atgccaatca
     4861 tcgatggatt ggccagggtt tcaaaatcct gcgcccacag ggcctgcagc aattgggtca
     4921 aaagttccat tcattattcc tggtgaatcg gttaacgcca cgctggtttt aaaagatgca
     4981 ctaccgcaat gttattgctg ttttggtatg ttctgcgggt ttgatacatc aacacagcct
     5041 gaactattcc ctgttaaatc gtgagataag aaagcattca aaaacataaa ttcaaattga
     5101 atctaattgt aacaaaggtc aacgttgttc gttatcaaaa ttacatgtcc gtggagtctg
     5161 attttgctga atgcagggga gggtggcgag aggatttaca caggctgaca ctttatactt
     5221 ttcgcttacc gacgagacaa acaggcgcat tgcagcgcct gtcggacagg aatttcattt
     5281 actgccgctg gtggctgtat cgagatgcac caccgggttt tcggcgaata gatagcggtc
     5341 ggcattgaac tcgaagtcgt cgctggtttc gttgaataac atgatcttgg tgttttccag
     5401 gtgctgccac attgccagct tagcagcatg aggatctttg cgaatcagcg ccttgaggat
     5461 ttgatcgtgg tcatcacacc agttatcgac ggtacgggaa tcaatgtgtt cgtgcagttt
     5521 tttccagtac gggttatggc tacgctgggt ccacattttt tcaacgatag ccgccagggc
     5581 ggagttctgc gtcgccaggg ctacctgaat atggaactgc aaatcccact cggaatcacg
     5641 gaagcattgt tcaccgcgtg cctgctcctg gatggccatc aatttcatga tgtcctgttt
     5701 cgttacctga gttgccgcga actcggcaat attactttcg atgagctggc gagcctggag
     5761 taactcaaac ggaccgtaat tggcgaattc catattattg tccgccgtct gctgatggcg
     5821 cggctggttg gaaaccacgt gaatacctga acctttacgc acttcgacat agccttcaac
     5881 ttccagcatg atgatggctt cacgaactac cgtacggctg acgttctttt catcggcaat
     5941 aaagcgctcg gcaggcagtt tatcacccac cagatagacg ccctgttcga tgcgctcttt
     6001 caggtcagcg gcaagttgtt gatacaaacg gcgtggttca gtgatttcca tatgcgctcc
     6061 aggcatgata cggcagacta tatttgttat accacttttg cgagtttagc tctactcgca
     6121 gtgatgaaaa agccgcctga gaaggcggct ttgaagggaa actaaaactt acacgaagcg
     6181 ttaatgttgc ggcgcgggat cgttatgtgt ttcctgcgcc acctcaatcg ccggtttgtt
     6241 ctgcaacacg gtccagataa ccagcgcacc cagcaggtca aatactgcca gaactgcgaa
     6301 cagcgggctg aagccgatgg tgtcagccag tgcaccgaca accagcgcaa acagcgtact
     6361 tgccagccat gcggacatcc cggttaaacc gtttgccgtt gccacttcgt tacgaccaaa
     6421 cacgtcagaa gagagcgtaa tcagcgcgcc agacagtgcc tggtgggcaa aaccaccgat
     6481 acacagcagc ataattgcga catacgggtt ggtgaacaga ccgatcatac ccgggccaat
     6541 catcagcaca gcacccagcg ttacgaccat cttacgggaa acgatcaggt tcacaccaaa
     6601 ccaacgctgg aacagcggcg gcaggtaacc accgaggata caaccgaggt cagcaaacag
     6661 catcggcatc caggcgaaca tcgcgatctc tttcaggtta aagccgtaaa ctttaaacat
     6721 gaacagcggg atccacgcgt taaaagtacc ccaggccggt tctgccagga aacgcggcag
     6781 cgcgataccc cagaactgac ggttacgcag gatctgacca acggacattt tcttcgccgt
     6841 gctcacctgg tgctgggctt cctgaccatt aataatatag tcgcgttctt catcggtcag
     6901 atgcttctgg tcgcgcggat gtttatagaa aatcagccat gccatcgccc agataaagct
     6961 caatgcaccg gagatgataa atgccatctg ccagctgtgc atcacgattg cccataccac
     7021 cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc ctactgcgat
     7081 ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg ggatcatcgc
     7141 tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc tacctgccag
     7201 cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac cgattttcgt
     7261 acccagcaca tccagtacat aacccgctac cggttgcatg accgtataag cagcagaata
     7321 ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca tcagagttgg
     7381 cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga gcgtcaccag
     7441 tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca tcgttttgtt
     7501 atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag ttacgaccgg
     7561 gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt gctaattcgg
     7621 cttccgtacc ggttaacccg attcatctaa caaatgagtg aatcggcaat ttgcattccg
     7681 tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa aaggtgagag
     7741 ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag gccagtactg
     7801 gcgcggcctg cagcgaaatt taccactttg agagtaattt ttttaactac gtttattgat
     7861 ctaactcacg aaaatatctt cggactctgg aaattggtgt gataactttg tcagcatcgc
     7921 accataagca agctagctca ctcgttgaga ggaagacgaa aatgactccg tttatgactg
     7981 aagatttcct gttagatacc gaatttgccc gccgtctgta tcacgactac gcaaaagacc
     8041 agccgatttt cgattaccat tgccatttgc cgccgcagca gattgcggaa gactatcgtt
     8101 ttaaaaacct gtatgacatc tggttgaagg gcgatcacta caaatggcgc gctatgcgta
     8161 ccaacggtgt ggctgagcgt ctgtgtactg gtgatgcgtc tgaccgtgaa aaatttgacg
     8221 cctgggcggc gactgttccg cacactatcg gcaacccgtt ataccactgg acgcacctcg
     8281 aactgcgtcg tccgtttggt atcactggca aattgctttc tccgtcaact gccgatgaaa
     8341 tctggaacga atgtaacgaa ctgctggcgc aggataactt ctccgcgcgc ggcatcatgc
     8401 agcagatgaa cgtgaaaatg gtcggcacca ccgatgaccc gatcgattct ctggagcatc
     8461 acgcagagat cgccaaagac ggctctttca ccatcaaagt gctgccgagc tggcgtccgg
     8521 acaaagcctt taacatcgaa caggcgacct ttaacgacta catggcgaag ctgggcgaag
     8581 tttccgatac cgacattcgc cgctttgctg acctgcaaac tgccttgacc aaacgtctgg
     8641 atcacttcgc cgctcacggc tgtaaagtgt ctgaccacgc gctggatgtg gtgatgtttg
     8701 ctgaagcgaa cgaagcggaa ctggacagca ttctggcgcg tcgtctggct ggcgaaaccc
     8761 tgagcgagca cgaagtggca cagttcaaaa ctgcggtgct ggtgttcctt ggtgccgaat
     8821 atgcacgtcg cggctgggta cagcagtacc acattggcgc gctgcgtaat aacaacctgc
     8881 gtcagttcaa actgctgggg ccggatgtag gctttgactc catcaacgac cgtccgatgg
     8941 cggaagagct gtctaagctg ctgagcaagc agaacgaaga aaacctgctg ccgaaaacca
     9001 ttctctactg cctgaacccg cgcgataacg aagtgctggg caccatgatc ggtaacttcc
     9061 agggcgaagg tatgccgggc aaaatgcagt tcggttccgg ctggtggttt aacgatcaga
     9121 aagacggtat ggaacgtcag atgacccaac tggcgcagct cggtttgctg agccgctttg
     9181 tcggtatgct gactgacagc cgtagcttcc tgtcatacac ccgtcacgaa tacttccgcc
     9241 gcattctgtg ccagatgatt ggtcgctggg tggaagcggg cgaagcaccg gcggacatca
     9301 acctgctggg tgagatggtg aaaaatattt gctttaacaa tgcgcgtgac tacttcgcca
     9361 ttgaactgaa ctaaggtctg gggttgatat gcaatacatc aagatccatg cgctggataa
     9421 cgtcgcggtc gctttagcgg atttggctga aggcacagaa gtcagtgtcg ataaccagac
     9481 tgttacgctg cgccaggatg ttgctcgtgg acataaattt gcgttaacgg atatcgcaaa
     9541 aggggccaac gtcatcaaat atggcctgcc gattggttat gcattggcgg atattgcggc
     9601 gggggaacac gttcacgccc acaatacgcg cacgaatctg agcgatctgg atcagtatcg
     9661 ctatcaacct gattttcagg atctgcctgc gcaagcggca gatcgtgaag tgcagatcta
     9721 tcgtcgcgct aacggcgatg tcggggtgcg taatgagctg tggatcctgc caaccgtggg
     9781 ctgtgtcaac ggcatcgcgc ggcagatcca aaagcgtttc ctgaaagaga ccaacaacgc
     9841 cgaaggtacc gacggcgtgt tcctcttcag ccacacctac ggctgctcac agctgggcga
     9901 cgatcacatt aatacccgca ccatgctgca aaacatggtg cgccacccga acgcaggcgc
     9961 agtgctggtg attggtctgg gctgtgaaaa caaccaggtt gccgcattcc gtgaaacgct
    10021 gggcgatatc gatcctgaac gcgttcattt catgatctgc caacagcagg atgatgagat
    10081 cgaggccggg atcgagcatt tgcatcagtt gtataacgtg atgcgcaacg ataaacgcga
    10141 gccaggcaaa ctcagcgaac tgaagtttgg tctggagtgc ggtggttctg acggtctttc
    10201 tggtattact gctaacccga tgctggggcg tttctctgac tacgtgattg ctaacggcgg
    10261 tactaccgta ctgaccgaag tgccggagat gtttggcgca gagcagttgc tgatggacca
    10321 ttgccgcgac gaagcaacgt ttgaaaaact ggtcactatg gtcaacgact tcaaacagta
    10381 ctttattgcc catgaccagc cgatctatga aaacccatcg ccggggaaca aagcgggcgg
    10441 tatcaccacg ctggaagaca aatcacttgg ctgtacccag aaagcgggtt ccagtgtcgt
    10501 ggttgacgtg ctgcgttacg gcgagcgtct gaaaactccg gggctgaact tgttaagtgc
    10561 gccgggtaac gatgccgtag cgaccagcgc cctggcgggt gcgggctgcc atatggtgct
    10621 gttcagtact ggtcgtggca cgccgtatgg tggatttgtg ccgacggtga aaatcgccac
    10681 caacagtgaa ctggcggcga agaaaaaaca ctggatcgac tttgacgcgg gtcagctgat
    10741 ccacggtaaa gcgatgccgc agttgctgga agaatttatc gacaccatcg ttgagtttgc
    10801 caacggtaag caaacctgta acgagcgtaa cgacttccgc gaactggcga tcttcaaaag
    10861 cggcgtaacg ctataaagtc gaaatgagtt gttaataaaa aacggcgttt cataatgtga
    10921 agcgccgttt gcttttccat acctgctaag gatttatcat gaccgcgtat tggctggccc
    10981 agggcgtggg tgtcatcgcc tttctgattg gtatcacaac atttttcaat cgtgacgaac
    11041 gtcgcttcaa aaagcagctt tcggtctata gcgccgttat tggcgtacat ttttttcttc
    11101 tgggcaccta tcccgctggt gccagtgcca tccttaatgc cattcgtaca ttgattacct
    11161 tacgcacgcg cagcttatgg gtaatggcga tttttattgt gctgactggc ggaattggcc
    11221 tcgcgaagtt ccatcatcct gtcgaactat tgccggttat cggcacgatt gtcagtacct
    11281 gggcgctgtt ccgctgtaaa gggctgacca tgcgctgcgt gatgtggttt tcaacgtgtt
    11341 gctgggtgat tcacaacttc tgggcggggt cgataggcgg cacgatgatt gagggtagtt
    11401 ttctgcttat gaatggcctg aatatcattc gtttctggcg gatgcagaaa aggggaattg
    11461 atccgtttaa agtagagaaa accccttccg ccgtagacga aaggggttaa acattaatta
    11521 cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc agtgaacagc
    11581 acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc gatgataaag
    11641 ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt acaggccagc
    11701 gggatcagca gcagagaacc acccgccacg ccggatgcgc cacaggcaca cagagaagcc
    11761 actacgctca acagcagcgc tgtgggcaga tcgaccggaa tacccagcgt attaaccgca
    11821 gccagcgtca acacggtaat ggtgattgct gcacccgcca tattgatggt ggcacccagc
    11881 ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag cgccatattc
    11941 accggaatgt tagctgcaga gctgcgggtg aagaaggcat acacgccgct ttcgcgcagg
    12001 cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag caatgggtta
    12061 accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg cgcgtagccc
    12121 cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagcccaaa aataccgatc
    12181 ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacat atcgttgacc
    12241 aggtttttgg tggtttcgtt accgtgacgc agtgcgaaac cgaggccaat tgcccacacc
    12301 aggatcccga tgtagttacc tttcagcagc gcgtcaatgg ggttggaaac catgctcatt
    12361 accagtccgc gcatcacttc gacaatgcct gacggcggcg aaatatcacc cgcgctactg
    12421 gacaagtgca gggtagaagg gaaggcaaag ctgaagacta ctgcggccag agctgcagag
    12481 aaggtgccca gcagatagag gaacaaaata ggacggatat tggttttctg cccgtgctgg
    12541 tggttagcaa tagatgccat taccagcatc aacaccagga tgggggcaac agctttcagt
    12601 gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt tgagatccat
    12661 gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct gccatgagcc
    12721 agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt tcattgtgtt
    12781 gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa agtgtttgca
    12841 aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat tttaaagaga
    12901 ggtcatgttt tgcaacttaa tggttacatt tatttgacat aaacagaggg aaggactggc
    12961 tactaaggct gattagataa catatgggca aaactatttg gaatatgaga acggttgcgc
    13021 tcctttaatg tgctatgcca tataaagtca ctggcacagg gg
//
LOCUS       DAZKFU020000129        12953 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZKFU020000129 DAZKFU020000000
VERSION     DAZKFU020000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.130"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..467
                     /locus_tag="ACHOQQ_RS14860"
                     /old_locus_tag="pgaptmp_002972"
     CDS             <1..467
                     /locus_tag="ACHOQQ_RS14860"
                     /old_locus_tag="pgaptmp_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU4471191.1"
                     /translation="SKLDLINFEHADLSRVNLNKATLQNINLIDSKLFFTRLTNTFLE
                     MVICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILR
                     GEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQDAD"
     gene            complement(621..2270)
                     /gene="yjcE"
                     /locus_tag="ACHOQQ_RS14865"
                     /old_locus_tag="pgaptmp_002973"
     CDS             complement(621..2270)
                     /gene="yjcE"
                     /locus_tag="ACHOQQ_RS14865"
                     /old_locus_tag="pgaptmp_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU4471192.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2421..3770)
                     /gene="ghxP"
                     /locus_tag="ACHOQQ_RS14870"
                     /old_locus_tag="pgaptmp_002974"
                     /gene_synonym="yjcD"
     CDS             complement(2421..3770)
                     /gene="ghxP"
                     /locus_tag="ACHOQQ_RS14870"
                     /old_locus_tag="pgaptmp_002974"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGU4471193.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4316..4780)
                     /gene="soxR"
                     /locus_tag="ACHOQQ_RS14875"
                     /old_locus_tag="pgaptmp_002975"
     CDS             complement(4316..4780)
                     /gene="soxR"
                     /locus_tag="ACHOQQ_RS14875"
                     /old_locus_tag="pgaptmp_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGU4471194.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSVRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4866..5189
                     /gene="soxS"
                     /locus_tag="ACHOQQ_RS14880"
                     /old_locus_tag="pgaptmp_002976"
     CDS             4866..5189
                     /gene="soxS"
                     /locus_tag="ACHOQQ_RS14880"
                     /old_locus_tag="pgaptmp_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGU4471195.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5192..6778)
                     /gene="pdeC"
                     /locus_tag="ACHOQQ_RS14885"
                     /old_locus_tag="pgaptmp_002977"
     CDS             complement(5192..6778)
                     /gene="pdeC"
                     /locus_tag="ACHOQQ_RS14885"
                     /old_locus_tag="pgaptmp_002977"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGU4471196.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCSPAHQKRMLNIIRGYLYINELIYAHDN
                     HFLCSSLIAPVNGYTIAPADYKRDPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7207..7488
                     /gene="yjcB"
                     /locus_tag="ACHOQQ_RS14890"
                     /old_locus_tag="pgaptmp_002978"
     CDS             7207..7488
                     /gene="yjcB"
                     /locus_tag="ACHOQQ_RS14890"
                     /old_locus_tag="pgaptmp_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGU4471197.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7587..8123)
                     /gene="ssb1"
                     /locus_tag="ACHOQQ_RS14895"
                     /old_locus_tag="pgaptmp_002979"
     CDS             complement(7587..8123)
                     /gene="ssb1"
                     /locus_tag="ACHOQQ_RS14895"
                     /old_locus_tag="pgaptmp_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGU4471198.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8378..11200
                     /gene="uvrA"
                     /locus_tag="ACHOQQ_RS14900"
                     /old_locus_tag="pgaptmp_002980"
     CDS             8378..11200
                     /gene="uvrA"
                     /locus_tag="ACHOQQ_RS14900"
                     /old_locus_tag="pgaptmp_002980"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGU4471199.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11235..11591)
                     /gene="yjbR"
                     /locus_tag="ACHOQQ_RS14905"
                     /old_locus_tag="pgaptmp_002981"
     CDS             complement(11235..11591)
                     /gene="yjbR"
                     /locus_tag="ACHOQQ_RS14905"
                     /old_locus_tag="pgaptmp_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4471200.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11595..12011)
                     /gene="yjbQ"
                     /locus_tag="ACHOQQ_RS14910"
                     /old_locus_tag="pgaptmp_002982"
     CDS             complement(11595..12011)
                     /gene="yjbQ"
                     /locus_tag="ACHOQQ_RS14910"
                     /old_locus_tag="pgaptmp_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGU4471201.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12122..12835)
                     /gene="aphA"
                     /locus_tag="ACHOQQ_RS14915"
                     /old_locus_tag="pgaptmp_002983"
     CDS             complement(12122..12835)
                     /gene="aphA"
                     /locus_tag="ACHOQQ_RS14915"
                     /old_locus_tag="pgaptmp_002983"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGU4471202.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3337 a         3407 c         3192 g         3017 t
ORIGIN      
        1 tctctaaact tgatttaatt aactttgaac atgctgatct gtctcgtgtc aacctgaata
       61 aagcaaccct ccagaatata aacttaattg atagcaaact cttttttacg cggttaacaa
      121 atacgttcct cgaaatggtt atatgtaccg gctctaatat ggctaatgtt aattttaata
      181 atgccaattt aagcaattgc catttcaact gttctatttt aacaaaagcc tgtatgttta
      241 atacccgtct ctatcgggtt aatttcgatg aggctagcgt ccagggaatg ggcatttcca
      301 ttctccgtgg ggaggaaaat atccccattg atagtgatac cctggtaaca ctacagaaat
      361 tctttgaaga agattgtacc tctcatactg gcatgtcaca aactgaagat aatattaatg
      421 cagtcgctat gaagattact gcagatatta tgcaagatgc agattgaggt taagccgaag
      481 aacagccaat gaattgactt tacaagagag ataacttatt tgttttagaa tcatgaatgt
      541 tagcccgagc taacctgtta aaggtgcaaa agattgcacc tttttcagat tacctcgggc
      601 ctggttacgt cgctttcggc ttactgattt tcctcaatca acaacgcttc aagcaaatcg
      661 agatcgtgca gtaatttttg cagcgtttcg ttactgatct cccgcgtggc gcgtaggtgg
      721 tacagttcag cacgttcaga acgcaatgcc gccagacgga agcgacgctc aaggttctct
      781 tcctgcacgg aactttcaac gtcgttacgt ccatcggcgc gacgacgcag gttaccaatg
      841 acgcgagaac tgacctccgt aagcagctgg ttatcgatat tctcttcggt atccgccgcc
      901 agacgctcct ccattttctg gatcgccaca atcgccactt ctgccgttgc cgctcgcgca
      961 atacgttctt ctttcaattg ttgcgaatgg tccgcgactt caatgtgttg tagcagaata
     1021 ggcaacatca ccacgccgac aaacagcgag aagagaatga caccagccgc cagaaacacc
     1081 agctcatagc gcgccgggaa gacgttacca tccggcaaga gcagcgggat agagagcaca
     1141 ccggccaaag tgatcgcccc acgcaccccg gcgaaagacg cgatcaggat ttctcgtgtg
     1201 gtccacgaac caaactccat cggcttcttc ttcaggaagc ggttgctgaa ctttttcatc
     1261 gtccacagcc agccgaaacg gaccagcatc agcgccgcat atatcagaat aatatcggta
     1321 aacagcatcc agatttcgac gttagggtcg atttctgctg ccatcagcga cgtctccaga
     1381 atacccggca gctgcagacc taacagcagg aataccatgc cgttaaagac aaattccagc
     1441 atcgcccagg tgctgtttgc acgcaggcgc attgccagcg gcgcacggcg catcacaccg
     1501 gagcgggtga tggtcatccc ggcggcaacc gcagcgagga tgccggaaac gccaatatgt
     1561 tccgcaatca gataagaagc gaatggcagc aggaacaaca ggacgatctg cgtcgcgggt
     1621 tcatcaccgc cccagcggct gaggaatcgc agcgagcgac cgtacagcca gctaaccaca
     1681 aaaccggcga gaataccgcc aatggctact ttcataaatt cgaccgtcgc accgccgacg
     1741 gtgaagatca tcgtccccat cgccactgct acggcaaact tcaacgacac caggccggaa
     1801 gcgtcattca tcaacgcttc gccctgcaaa atgcccatga tttttttcgg gatgcgccct
     1861 tcgccaacaa tcccggagag cgccacagca tcggtcggag aaagtaccgc cgccagcgca
     1921 aaggcgggga tcagcggaat gcccggcacc acccagtaaa taaggaagcc aatgccgacc
     1981 acggtgacca ccaccagcgc cagtgcgagg ccgaaaatct ctcgaccatg ttcgagaaat
     2041 tcacgggtcg gcgttttcca gccatcagcg aacagcaacg gcgggataaa caagactaaa
     2101 aagagttcag gatcaaactc cacatgcaaa ccaaacgtcg gccacgccag tagcgcaccg
     2161 atggcgattt gcataagcgg aagcgggatc tgaaagggca tgacacgagt gaccaccccg
     2221 gacagcgaga ccacgagggt cattatcagt atggtgaaga agatttccat gcgttccctg
     2281 tttgcgatgt tttttatgat ctgagaaaga cgttatgcct gaattctcat tttttcagaa
     2341 tatcgcaaaa gctcacacaa tgtgcggaga aaataaaaac gggcagccat tgccacccgt
     2401 tttcagaatc cgatgaaaga ttagatagcc cagccacccg catagaaggt caccagcgcc
     2461 acggcgataa cgaccgtacc gatgttcaac ttgcgccatt cgccggaaac cagacgacca
     2521 atcaccaggg tcgcgaagcc gatcatgatg cctgttacga tgttacaggt cagcacgatg
     2581 aataccgccg taaccagacc cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc
     2641 acgttgctca gcattagcag gccaacgtac atcagcgccg gagccgttgc ataccccgga
     2701 acgaggtaag aaagcggaga gaggaacaga atcagcagga acagcacgcc aacggtgata
     2761 gccgtcaacc cggttttacc gcccgccgcc gtacccgccg cagactcgat gtataccgct
     2821 gccggagctg cacccaccag gccagagaaa acgctgctca tggagtcagt ggtcagtgct
     2881 ttcccgccgt cgatgatctg cccatcttta tccagcaggt tcgcctggcc ggcgacggca
     2941 cggatagttc cggtggcatc aaatactgcc gtcatcacca gcgccagaac gcttggcagg
     3001 actacaggat tcagcgcgcc cataatgtcc aggctgccaa tcagtgaatt gccgttttca
     3061 tcgctcaatg aaggcatggc gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa
     3121 ccgacaattg agataccgat aatggtcagc agaatgccac cagggacttt cagtttttcc
     3181 aggccgatga tcaccgccag acctaccagt gacataatca ccgggaaggt cgcgaaatca
     3241 cccagcgcaa cgggcagacc atcaagcggg tttttaatca ccagaccgac accgttagcg
     3301 gcaatgagca gcaggaacag accgataccg atccccgtgc cgtgcgccac accgtgcggc
     3361 aagttgcgca aaatccagct acggataccc gtggcagaaa ttaccgtaaa cagcacaccc
     3421 atcaggaaca cggcacccag cgcgacaggt acgctaatat gttgccccag caccaggctg
     3481 aatgcggtaa acgccgtcag ggagatggcg caaccaatcg ccaacggcag attagcccac
     3541 agacccatca cgatagaacc gagtccggca accagacagg tcgcaacgaa aactgccgca
     3601 ggcgggaagc ccgctttacc caacatgcct ggaacgacga taaccgagta gaccatcgcc
     3661 agaaacgttg ttaacccggc aaccacttcc tgacggacag tgcttccacg ttgtgaaatt
     3721 ttaaaccagg cgtcgagtga accgccggta cgcgctgatg gcgtagacat aagagaacat
     3781 cccctgagag tttaattttc gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt
     3841 tatcttcctg ctccaggttt gcgacaatgt gctgcgtcgc aaaaaagcaa acgtttagct
     3901 ccgcgcatac aaaacgggtg gtaaaaaaag caaacgatta tccggcgatc atagcggctt
     3961 tttcaaccga agtttgctgc ttttttctct tttattgtct tgacgatgaa aaaccaggca
     4021 attgatgcgc aaacgttatc gtcgatgcgc aagattgcag cgttatcgag ccgtataccg
     4081 ttctggattg cgataaaaaa tcgtctgaga tgacaaaaat attgcggcta aaaagtatac
     4141 ttttacactg gcgaagaaac gccggtgaca tacaattaaa gcatcaacac caaccggaac
     4201 ctccaccacg tgctcgaatg aggtgtgttg acgtcggggg aaaccctcct gtgtaccagc
     4261 gggatagaga gaaagacaaa gaccggaaaa caaactaaag cgcccttgtg gcgctttagt
     4321 tttgttcatc ttccagtaag cgtgcgccgg taccttcttc tcctaagcgg tcgcccgggt
     4381 tacgcaacgg gcaatcactg cgcgaaaggc agccacaacc aatacatcca tccagttcgt
     4441 cacgcagcgc cactaaggta tgaatgcgcc gatccaactc ttctcgccat tgggacgaaa
     4501 gctgtttcca ctctttcgca cttaacgtat gcccttcggg caacacgcca aacgcttcac
     4561 caatggtcgc cagcggaatg ccaatacgct gagcaatttt gataattgcg acatatcgca
     4621 acacatcacg tttatatcgg cgctgattgc cgctattacg gacactggta atcaaccctt
     4681 tgctttcata gaaatgcagc gccgataccg ccacaccgct gcgtttcgcc acttcgccgg
     4741 gggttagcag cgctttaatg cggggtaatt tcttttccat aaatcgcttt acctcaagtt
     4801 aacttgagga attatactcc ccaacagatg aattaacgaa ctgaacactg aaaagaggca
     4861 gatttatgtc ccatcagaaa attattcagg atcttatcgc atggattgac gagcatattg
     4921 accagccgct taacattgat gtcgtcgcaa aaaaatcagg ctattcaaag tggtacttac
     4981 agcggatgtt ccgcacggtg acgcatcaaa cgcttggcga ttacattcgc caacgccgcc
     5041 tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc gatttttgat atcgcaatgg
     5101 acctgggtta tgtctcgcag cagaccttct cccgcgtttt ccgtcggcag tttgatcgca
     5161 ctcccagcga ttatcgccac cgcctgtagt tttattgccc gcgcgttaac tcccgcgcgg
     5221 gtaattgctc catccattgc ataaacacct gcggcggcat cgccttcgca aaaaaccatc
     5281 cctggcaata gcgcacgccg cgtttgcgca gccagttaac ctgctcctcc gtttcgacgc
     5341 cttcagcgat cgtttttaac cccaggctgt gcgccagctc gatgatatgt tccgcaatca
     5401 aatgactggt tttatgggtg gtcagcgtct caacaaacga tttgtcgatt ttcaaaatat
     5461 cgacattcaa tgatttaagg ttatgcaagt tagagtagcc aataccaaaa tcatcaattg
     5521 ccacttcgta accggcctgg cggaaagcca gaataatcgg cgtcattttg tcgacatcaa
     5581 gaaatgcatg ttcagtcact tcaaatttaa tttgctgtgg acgcaccgcg tattgctctg
     5641 ttttctgatt gattcgcgct atcaaccgtg acgtatgaaa atcggaggcc gacagattaa
     5701 tagaaacata gcgatcagca tgtgttgcaa ggtacgcgcc cagatcgcgg aagacattat
     5761 caataacata atcagttacc tgttctatca tcccctcctt ttctgccagc ggaataaact
     5821 ctgccggatt cattacttgc ccctgctcac caggccaacg taacaacgct tcagcgccga
     5881 tacatttttc tgttttgata tcgattattg gctggtaata aagacaaagt tgatgttttt
     5941 ctagggcgcg ttgcaatttg cgtttgggag acaaatagtt ttgtcgaata cgtagccaga
     6001 gtaatagcag aacaagactc cccaaaatac cggcgggtaa cgcaaaaata agatgattat
     6061 aaaaatgggc tataagacgt tgatatgaag tcgcaacaat ggctgcaatt gggcgttttg
     6121 tcgaataaac tgtcgcatat aaatagccat ttctttgtac ggttaaatcc ttcaaatgaa
     6181 tcagcggaga aaacgttgct gccgaggcct cgttgcttaa cgagaaaaag gttttcgtca
     6241 ccgtatcata cacaccccat tgcaatgtcg ggtcatcaga catcacttca ctccagaaga
     6301 gagggttgat aaccgccaca taatttcccc gctgcatata ggtcatttta tagccagaga
     6361 aaaaaggcgt atcgcggtaa taatagatag aaacgttagg gtcacgctta taatcggccg
     6421 gtgcaatcgt atagccgttt acaggcgcta tcagcgatga gcataaaaaa tggttatcat
     6481 gggcatagat caattcatta atataaagat agccacgaat aatatttaac attcgctttt
     6541 gatgggctgg agagcaaact tgcccctgat agcgttctgc tgcatcgctc actaaatctg
     6601 cctggcgaat gaccagctcg gatttatcca gtgccagttg agcaaaggtt cggagctgat
     6661 tattcacttc tgattttgcc caaaggaagg caatccatag cgaaagaatg atgggaaaga
     6721 gaactaaaaa gataatgccc ggcaacgcca gtaattggtg tcgtgcacga tgactcattg
     6781 ctatcaattt cctgcctgtt tatacccggc cattgcaagg tgaaaccctg gctaccgacg
     6841 tctttagata aatcttacgg tataaatagc acttttgcct gatttgaaat tgataccttt
     6901 ttaatcgtca cctggatgaa aataattcat tgatattcaa aattaatttt ctacaggaat
     6961 acagaggcgg cgggaagata tacccttaat gccactgaaa aaccacataa tctgtacagc
     7021 aataaaagtc acgacctaat ctggctaaca aacaatcaat tgtgatatag ttcacaaaat
     7081 taatgaaaca aacagagtgt ttcatttttg tgttctgcaa gccccgcgat agccagagtg
     7141 taccacgcct cccgtgaaca acgccgcgct gtccagggca tcggctcttt tcagaggaat
     7201 tgtttgatgg ctaccctcac cactggcgtg gttcttcttc gctggcaact tcttagtgcc
     7261 gtaatgatgt ttctggccag cacgctcaac atccgttttc gtcggtctga ttatgtcggg
     7321 cttgcagtga tcagcagcgg tctgggcgtg gtttccgcct gctggttcgc gatggggttg
     7381 cttggcatca caatggcgga tatcaccgcc atctggcaca acatcgagtc ggtgatgata
     7441 gaagagatga atcagacacc gccacaatgg ccaatgattt tgacctgata cgtagaagcc
     7501 tccataacgg aggcttcttt tttatggctg gcagatgctt taatcatcca ccttaaaaca
     7561 atataaccta ttgttttaat gacaaatcag aacggaatgt catcatcaaa gtccatcggc
     7621 ggctcgttag acggcgctgc cggagcggac tgctgcgggc gagactgcgc gccgccgctg
     7681 aactgattgc caccctgcgg ctgctgaggc tgaccccaac cgccctgcgg ctgaccacca
     7741 ccgatattgc cacctgcagg agcgccacca ccctgacgac cacccagcat ctgcatagtg
     7801 ccgccaacgt tcaccacaac ttctgtggtg tagcgatcct gaccagattg atcggtccat
     7861 ttacgggtac gcagctgacc ttcgatataa acctgagaac ctttacgcag atattcgctg
     7921 gccacttctg ccagtttgcc gaacagcaca acgcggtgcc attcagtctg ctctttcatc
     7981 tcgccggtcg ctttatcacg ccaggattcg gaagtagcca gcgtaatgtt ggcaactgcg
     8041 ccaccatttg gcatgtagcg tacttccggg tcctgaccca gattaccaac gagaataacc
     8101 ttgtttacgc ctctgctggc catgttcgtg tctcctgaaa aaaaatcgtt ctgaataagt
     8161 gtaaacgtgc gattgtacca ttaccaatag cgcttttact atgttgtgac ctcggttccg
     8221 ggaaacaaac ctggccagac attgttacac aacactccgg gtaatgcatt ccaatactgt
     8281 atattcattc aggtcaattt gtgtcataat taaccgtttg tgatcgccgg tagcaccatg
     8341 ccaccgggca aaaaagcgtt taatccggga aaggtgaatg gataagatcg aagttcgggg
     8401 cgcccgcacc cataatctca aaaacatcaa cctcgttatc ccccgcgaca agctcattgt
     8461 cgtgaccggg ctttcgggtt ctggcaaatc ctcgctcgct ttcgacacct tatatgccga
     8521 agggcagcgc cgttacgttg aatccctttc cgcctacgcg cggcagtttc tgtcactgat
     8581 ggaaaagccg gacgtcgatc atattgaggg gctttctcct gccatctcaa ttgagcagaa
     8641 atcgacgtct cataacccgc gttctacggt ggggacaatc accgaaatcc acgactattt
     8701 gcgtttgttg ttcgcccgcg tcggcgaacc gcgctgtccg gaccacgacg tcccgctggc
     8761 ggcgcaaacc gtcagccaga tggtggataa cgtgctgtcg cagccggaag gcaagcgtct
     8821 gatgctgctc gcgccaatca ttaaagagcg caaaggcgaa cacaccaaaa cgctggagaa
     8881 cctggcaagc cagggttaca tccgtgctcg tattgatggc gaagtctgcg atctttccga
     8941 tccgccgaaa ctggaactgc aaaagaaaca taccattgaa gtggtggttg atcgcttcaa
     9001 ggtgcgtgac gatcttaccc aacgtcttgc cgagtcgttt gaaaccgcgc tggagctttc
     9061 cggtggtacc gcggtagtgg cggatatgga cgacccgaaa gcggaagagc tgctgttctc
     9121 cgccaacttc gcctgcccaa tttgcggcta cagtatgcgc gagctggagc cgcgactgtt
     9181 ttcgtttaac aacccggcag gtgcctgccc gacctgtgac ggccttggtg tacaacaata
     9241 tttcgatcct gaccgcgtga tccaaaaccc cgagctatcg ctggctggcg gtgcgatccg
     9301 tggctgggat cgccgcaact tctattattt ccagatgctg aaatcgctgg cagatcacta
     9361 taagttcgac gtcgaagcgc cgtggggcag cctgagcgcg aacgtgcata aagtggtgtt
     9421 gtacggttct ggcaaagaaa acattgaatt caaatacatg aacgatcgtg gcgatacctc
     9481 cattcgtcgt catccgttcg aaggcgtgct gcacaatatg gagcgccgtt ataaagagac
     9541 ggagtccagc gcggtacgcg aagaattagc caagtttatc agtaatcgtc cgtgtgccag
     9601 ctgcgaaggg acgcgtctgc gtcgggaagc gcgccacgtg tatgtcgaga atacgccgct
     9661 gcccgccatc tccgacatga gcattggtca tgcgatggaa ttcttcaaca atctcaaact
     9721 cgcaggtcag cgggcgaaga ttgcggaaaa aattcttaaa gagatcggcg atcgtttgaa
     9781 attcctcgtt aacgtcggcc tgaattacct gacactttcc cgctcggcag aaacgctttc
     9841 tggcggtgaa gcacagcgta ttcgtctggc gagccagatt ggtgcgggcc tggttggtgt
     9901 tatgtatgta ctggacgagc cgtctatcgg cctgcaccag cgcgataacg agcgcctgtt
     9961 gggtacgctt atccatctgc gcgatctcgg taataccgtg attgtggtgg agcacgacga
    10021 agacgcgatt cgcgccgctg accatgtgat cgacattggc ccgggcgcag gtgtacacgg
    10081 cggtgaagtg gtcgcggaag gtccgctgga agcgattatg gcggtgccgg agtcgttgac
    10141 cgggcagtac atgagcggta aacgcaagat tgaagtaccg aagaaacgcg ttccggcgaa
    10201 tccggaaaaa gtgctgaagc tgacaggcgc acgcggtaat aacctgaaag acgtgacgct
    10261 gacgctgcca gtcggtctgt ttacctgcat cacaggggtt tcaggttccg gtaaatcgac
    10321 gctgattaac gacacactgt tcccgattgc ccaacgccag ttgaatggtg cgaccatcgc
    10381 cgaaccggca ccgtatcgcg atattcaggg actggagcat ttcgacaaag tgatcgatat
    10441 cgaccaaagc ccaattggtc gtactccgcg ttctaacccg gcgacctata ccggcgtgtt
    10501 tacgcctgtg cgcgaactgt ttgcgggcgt accggaatcc cgtgcgcgcg gctatacgcc
    10561 gggacgtttc agctttaacg tccgtggcgg acgctgcgag gcctgtcagg gcgatggcgt
    10621 gatcaaagtg gagatgcact tcctgccgga tatctacgtg ccgtgcgacc agtgcaaagg
    10681 taaacgctat aaccgtgaaa cgctggagat taagtacaaa ggcaaaacca tccacgaagt
    10741 gctggatatg accatcgaag aggcgcgtga gttctttgat gcggtgccag ctctggcgcg
    10801 taagctgcaa acgttgatgg acgttggtct gacgtacatt cgcctggggc agtccgcaac
    10861 cacactttct ggtggtgaag cccagcgcgt gaagctggcg cgtgagctgt caaaacgcgg
    10921 caccgggcag acgctgtata ttctcgacga gccgaccacc ggtctgcact tcgccgatat
    10981 tcagcaactg ctcgacgtac tgcataaact gcgcgatcag ggcaacacca ttgtggtgat
    11041 tgagcacaat ctcgacgtga tcaaaaccgc tgactggatt gtcgacctgg gaccagaagg
    11101 cggcagtggt ggcggcgaga tcctcgtctc cggtacgcca gaaaccgtcg cggagtgcga
    11161 agcatcacac acggcacgct tccttaagcc gatgctgtaa tcgttaaggc cgctttctga
    11221 gcggcctttt cctttcagag ttgcaccagc aatttacgtt tttcttccgg cagtaaattc
    11281 accgcctgct gataagacgc atccaccaga taatagattt gcgaatctgg cagcgaaccg
    11341 tcgagataca cggtgctcca gtgcgcttta ttcagatggc ggcttggacg cacatcgctg
    11401 tgctgctgac gtagcagctc cgccagctcc gggctggttt tcagcgaaac agctgggcga
    11461 ttttctactt ctttcaccat cgcaaacagt acatcttcaa ctttgatctg cgtcgctttc
    11521 cagtcgttat gcacgctctg ttctgcgcct ggttttgcca tgcaatattg tagcaactcc
    11581 gaaatggtca ttttttactc cccttgtagt gtcgcgatga tgcgacgcga tccgccgtgg
    11641 atgcgatgtt cccccagcca aatgccttgc caggtgccgg tctgaatacg ccctttatgc
    11701 accggcaata caagcgatgt tcccagcatt gaggatttga tatgagaagg catatcgtct
    11761 gctccctcat agtcatgctc atagtttcca ttatcgggaa cggtgcggag gaaaaaacgc
    11821 tccatgtcgt ggcgtacggt gggatcgcag ttctcattaa gtgtcagaga ggcggaggta
    11881 tgttgcagca acagatgcag taagccgatg ttaacgcgcg gcatatcagc cagctgattc
    11941 agaatttcat ccgttaccag atgaaaccca cgagatttgg cgctaagcgt gagcgtcttt
    12001 tgataccaca tgtgctgctc cttgataaaa ctctcttaat cagtttgcag caagacagcg
    12061 aaaggataaa ggtgtgatta aaaaaacagc attcgggaga gtgtcacgct ctcccgttct
    12121 gtcagtattc tgaattgacg atcacctctt caccaaacgc acccgcttgt ggcaagggtt
    12181 tgtaggtaga gttggaggcg cgcagaatgc ggataccacg agcgccgaca tcgcgtgcag
    12241 cggtaatatc gttatcggaa tcgccataaa aaattcggat atttttatcc tgcagccatt
    12301 gcgattttgt attttgccct ggtttatcgc ccgcaaagat caccggattc atgttggtgg
    12361 caggaatatg aaaattatcc gccagcgttt ttgaaaccgt ttctgttttc gtcgggctac
    12421 gaccagtcac aaagaagatc gcgtcaccac ggcgtacatg catatcaatc agctggcgag
    12481 cgacctcttt tggaatgctg aattcatccc agccgttgtt cattttttcc cagaatacag
    12541 ggtttttcag ataatcttcg ctttctggcg agaaggtttt tttgccgcgc cagaagcccg
    12601 ggctggaaaa aagtaccgtg tcatcgatat caaaccccac cgccattggc ggacgccctg
    12661 cgaggctatt ttcaatttgt gcgaccgaaa cccaatgaat tggtgcctgt tcagcaagcc
    12721 tggcaacgtt agtcccaggg ttaagcggtg aaggagatga ggccagggca acagcggaac
    12781 tgtttagagc gaacaataag caaacggcac tgattgcctg tgtgatcttg cgcatatttt
    12841 tccctaaata gtcagtatgt tgaaactttt tattgtgaga tttgttgcaa aaaccatctg
    12901 accataacgc cagctgcagg ggaaaggaag gattatctgc ggtttttgtg atg
//
LOCUS       DAZKFU020000130        12741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZKFU020000130 DAZKFU020000000
VERSION     DAZKFU020000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.131"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..964
                     /gene="ynjI"
                     /locus_tag="ACHOQQ_RS14920"
                     /old_locus_tag="pgaptmp_002984"
     CDS             <1..964
                     /gene="ynjI"
                     /locus_tag="ACHOQQ_RS14920"
                     /old_locus_tag="pgaptmp_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4471203.1"
                     /translation="FERMIKENKAMLLCKWGFYLTCVVAVMFVFAAITSNGLNERGLI
                     TAGCSFLYLLITMGLIVRAGFKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHK
                     QWSETLEFWPRKPEPGKDTFQYHVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEH
                     FLSGDHGANTFKANMEEAPEQVIALLNKFAVFPSDYISDCANHSSGKSSAKLIWAAEL
                     SWMISISSTAFQNGTIEEELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCY
                     RRKLTDAELEACYRYDKQFWEHYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(1081..2424)
                     /gene="gdhA"
                     /locus_tag="ACHOQQ_RS14925"
                     /old_locus_tag="pgaptmp_002985"
     CDS             complement(1081..2424)
                     /gene="gdhA"
                     /locus_tag="ACHOQQ_RS14925"
                     /old_locus_tag="pgaptmp_002985"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGU4471204.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGKQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2660..2932
                     /gene="ynjH"
                     /locus_tag="ACHOQQ_RS14930"
                     /old_locus_tag="pgaptmp_002986"
     CDS             2660..2932
                     /gene="ynjH"
                     /locus_tag="ACHOQQ_RS14930"
                     /old_locus_tag="pgaptmp_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGU4471205.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLNTNPLVWQRVKP"
     gene            complement(2898..3305)
                     /gene="nudG"
                     /locus_tag="ACHOQQ_RS14935"
                     /old_locus_tag="pgaptmp_002987"
     CDS             complement(2898..3305)
                     /gene="nudG"
                     /locus_tag="ACHOQQ_RS14935"
                     /old_locus_tag="pgaptmp_002987"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGU4471206.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            3392..4012
                     /gene="ynjF"
                     /locus_tag="ACHOQQ_RS14940"
                     /old_locus_tag="pgaptmp_002988"
     CDS             3392..4012
                     /gene="ynjF"
                     /locus_tag="ACHOQQ_RS14940"
                     /old_locus_tag="pgaptmp_002988"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4471207.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPTCFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(4021..5328)
                     /gene="ynjE"
                     /locus_tag="ACHOQQ_RS14945"
                     /old_locus_tag="pgaptmp_002989"
     CDS             complement(4021..5328)
                     /gene="ynjE"
                     /locus_tag="ACHOQQ_RS14945"
                     /old_locus_tag="pgaptmp_002989"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HGU4471208.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVNTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TEKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIRPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(5395..6048)
                     /gene="ynjD"
                     /locus_tag="ACHOQQ_RS14950"
                     /old_locus_tag="pgaptmp_002990"
     CDS             complement(5395..6048)
                     /gene="ynjD"
                     /locus_tag="ACHOQQ_RS14950"
                     /old_locus_tag="pgaptmp_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4471209.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALPGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDFQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(6048..7583)
                     /gene="ynjC"
                     /locus_tag="ACHOQQ_RS14955"
                     /old_locus_tag="pgaptmp_002991"
     CDS             complement(6048..7583)
                     /gene="ynjC"
                     /locus_tag="ACHOQQ_RS14955"
                     /old_locus_tag="pgaptmp_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGU4471210.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLMIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVILLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDRSWTAVVWGHLLWVIPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(7556..8722)
                     /gene="ynjB"
                     /locus_tag="ACHOQQ_RS14960"
                     /old_locus_tag="pgaptmp_002992"
     CDS             complement(7556..8722)
                     /gene="ynjB"
                     /locus_tag="ACHOQQ_RS14960"
                     /old_locus_tag="pgaptmp_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4471211.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVQEDFSVPTEGAESPWGGAQLTFIARRD
                     VPPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTADPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKSGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(8732..9280)
                     /gene="ynjA"
                     /locus_tag="ACHOQQ_RS14965"
                     /old_locus_tag="pgaptmp_002993"
     CDS             complement(8732..9280)
                     /gene="ynjA"
                     /locus_tag="ACHOQQ_RS14965"
                     /old_locus_tag="pgaptmp_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU4471212.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(9280..9987)
                     /gene="ydjZ"
                     /locus_tag="ACHOQQ_RS14970"
                     /old_locus_tag="pgaptmp_002994"
     CDS             complement(9280..9987)
                     /gene="ydjZ"
                     /locus_tag="ACHOQQ_RS14970"
                     /old_locus_tag="pgaptmp_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4471213.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(10002..10679)
                     /gene="ydjY"
                     /locus_tag="ACHOQQ_RS14975"
                     /old_locus_tag="pgaptmp_002995"
     CDS             complement(10002..10679)
                     /gene="ydjY"
                     /locus_tag="ACHOQQ_RS14975"
                     /old_locus_tag="pgaptmp_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGU4471214.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VIFKITE"
     gene            complement(10684..11394)
                     /gene="ydjX"
                     /locus_tag="ACHOQQ_RS14980"
                     /old_locus_tag="pgaptmp_002996"
     CDS             complement(10684..11394)
                     /gene="ydjX"
                     /locus_tag="ACHOQQ_RS14980"
                     /old_locus_tag="pgaptmp_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4471215.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFKAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTAIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            complement(11561..12367)
                     /gene="xthA"
                     /locus_tag="ACHOQQ_RS14985"
                     /old_locus_tag="pgaptmp_002997"
     CDS             complement(11561..12367)
                     /gene="xthA"
                     /locus_tag="ACHOQQ_RS14985"
                     /old_locus_tag="pgaptmp_002997"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGU4471216.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         3134 a         3299 c         3246 g         3062 t
ORIGIN      
        1 ttttgaacgg atgattaaag aaaataaggc catgctgctt tgtaagtggg ggttttattt
       61 aacatgtgtt gtcgctgtaa tgtttgtatt cgcagcgata acatccaacg gtttgaatga
      121 aagaggcttg attaccgcgg gatgctcttt tctttatcta ttaattacga tggggcttat
      181 tgttcgggcc ggttttaaag caaaaaaaga acaactgcat tattatcagg ctaaaggtat
      241 tgagccgctc agtatcgaga agttacaggc gctacaattg atcgcacctt atcgattcta
      301 tcataagcaa tggtctgaaa cgctggagtt ctggccgcga aagcctgaac ctggcaaaga
      361 taccttccaa tatcatgtgc ttccttttga ctcgatcgat atcataagta aaagacgcga
      421 gtctttagag gatcaatggg gtatcgaaga tagcgaaagt tattgtgcct taatggagca
      481 ttttctttct ggcgaccatg gagccaatac ctttaaagca aacatggagg aagccccaga
      541 gcaggttatc gccttgttga ataaatttgc tgtttttccc tcagactata tctctgattg
      601 cgctaatcat agctccggta aatcctcggc gaagctaata tgggcggcgg aattatcatg
      661 gatgatctcg atatcaagca cagcttttca aaacgggaca attgaagaag aactggcctg
      721 gcattatata atgcttgcct ctcgaaaggc gcacgagttg ttcgaaagcg aagaagatta
      781 tcaaaaaaat agtctaatgg gatttcttta ctggcatatc tgctgctatc gcagaaagtt
      841 aacggatgct gaacttgaag cgtgttatcg ttacgataag cagttttggg agcactacag
      901 taagaaatgc cgttggccca taagaaatgt tccctgggga gcatcatccg ttaaatactc
      961 ataaacgcct gaaattttgc cgggggcgct gcgcttgccc ggcctacatt agatcgtaac
     1021 tgcaatgaat tgtgcccatt tgtaggcctg ataagcgtag cgccatcagg catttacaac
     1081 ttaaatcaca ccctgcgcca gcatcgcatc ggcaaccttc acaaaaccgg caatgttcgc
     1141 gccctgcacg tagtttgttt gcttaccttc accaccatgc tcaacacagg catggtggat
     1201 atccagcatg atgtgatgca aacgtgcgtc aactttctcg gctttccagc ccaggcgcgc
     1261 agcgttttgt gccatttcca ggcctgacgt tgctacaccg ccagcattag ccgctttacc
     1321 cggtgcaaac agtacgcctg cctgctggaa cagttcagtc gcttcgatgg tggtcggcat
     1381 atttgcccct tcggcgacgg ctttaacgcc attggcgata agctgatgcg cggcgtcaac
     1441 atccagttcg ttctgggtgg cgcaaggcag ggcgatatca accggtacag accacggctg
     1501 ttggccttcg agatagacca gaccaaattc tttggcgtaa tctgccactc gaccatcgcg
     1561 gctggatttg atttcgataa gacgtgccag tttctctttc gtgaatccgc tttcatcaac
     1621 tacagtgccg ctggagtctg acgcagtgat cacacgagca ccaaattcca tcgctttttc
     1681 gatagcgtac tgggcgacgt tgccggagcc agaaacggaa acgcgcattc cttcaaaacc
     1741 cataccgtgg cgttttagca ttgcttcggt gaaataaacc agaccgtagc cggtagcttc
     1801 cgggcgaata agactgccgc caaatgaaag acccttaccg gtgaatacgc aggcggtatt
     1861 gttggagagc tttttcatca tccccgccat aaagccgact tcacgaccac caaccccgat
     1921 atcacctgcc ggaacgtcgg tatccgcgcc caggtggcga tacagttcag tcatcagcgc
     1981 ctggcagaaa cgcatcactt cgccttcgct tttccctttc gggtcgaaat cgctgccgcc
     2041 tttacctccg cccatcggca gagtagtcag ggcatttttg aaggtttgtt cgaagccgag
     2101 gaatttgaga atggagaggt taactgacgg atggaagcgc ataccgcctt tgtacgggcc
     2161 gatggcagag ctgaactgca cacgccatgc acggttgacc tgtacctggt tgcgatcatc
     2221 aacccatacc acgcgaaact ggatcacgcg ctccggttca accagacgct ccagtaatga
     2281 catctggcga tattttggat tttgttcaag aaaaggccag agtgtggtca ttacttcacg
     2341 aacggcttgc gcgaactcgg tttgattcgg gtcgcgcttt tggacatggt tgaggaatga
     2401 ctccagagaa tatgtctgat ccatagatat aaaaccctta tatattaata cgattgtgct
     2461 tatgttgttt atgtcatgtg cttttgcagt tttcgactat accatcaaga atgtgacggt
     2521 aagcaagaaa aaaatgaccc aggaaagcaa attaataacg agagtaatct cataaccaaa
     2581 tgcgataact cagacgctaa agttttgcca cccggctacc gttatcagta gcagagacaa
     2641 taacgttaag gagttccctg tgagtcgagc attgttcgcc gtagtattag catttccatt
     2701 aattgcactg gctaacccgc attatcgtcc ggacgtggag gtaaacgtac cgccagaagt
     2761 gttcagttca ggcgggcagt cagcgcagcc ctgtactcag tgctgtgttt atcaggataa
     2821 aaattattct gaaggagcgg taattaaagc cgagggaata ctgctgcaat gccagcgtga
     2881 tgacaaaacc cttaacacta atccgctggt ctggcagcgc gtaaagccat aaatgcctct
     2941 aatagtggaa tgtcagcagg ggccagcgga tattgcagcg cctcttcagg tgagcaccag
     3001 accagcgcct gatgttcatg tgcctgtaac gtcccgtgga agtcgggtac gtgccaggca
     3061 tgaagatgga taatccgccc cgaaacttct cgctgatggc tggcaacata ctcacccaca
     3121 gttgcttcga tgcccaattc ttcgtttaac tcacgcacca gcgcctgctg ctggctttca
     3181 tccagctcga ctttaccacc ggcaaactcc cataatcccg cctgatcgct ctgggcgggg
     3241 cgttgcgcga gtaaaatttt gccatcacgt tcaatgatgg cggcaacaac ttcaatcatt
     3301 ttcataaatt acacacgggc agataagcga aaaatgccat aatatccgca ctgttgctca
     3361 atcagaaagg ctttatattt aaggagtgcc tgtgctagac cgccatcttc atccccggat
     3421 taaaccgttg ttgcatcagt gcgtgcgggt tcttgataaa ccgggcatta cgccagatgg
     3481 tttaacatta gtgggatttg ccatcggcgt gctggctctg ccgtttctgg ctctgggctg
     3541 gtatctggcg gcgctagtcg tcattttgtt gaatcgtctg cttgatggtc tggatggcgc
     3601 actggcgcgg cgcagaggat taaccgatgc aggcgggttt ttagatatct cgctggattt
     3661 tctcttttac gcgctggtgc cgtttggttt tattcttgct gcaccggagc aaaatgcgct
     3721 ggcaggcggc tggttgttgt ttgcgtttat cggtacgggc agcagttttc tcgcctttgc
     3781 tgcactggca gcgaaacatc agattgataa tcccggttat gcgcataaat cgttttatta
     3841 tctgggcggg ttaaccgaag gcagcgagac gattttgctg tttgttctga gttgcctctt
     3901 tccgacctgt tttccgtggt ttgcgtgggt atttggtgcg ttgtgctgga tgacgacctt
     3961 tacgcgggtg tggagtgggt atctgacgct gaagtcgctc cagcgtcagt aattgcaatg
     4021 ttatttgctg ctgtccgggc cgcgttcacc ggttgcaacc ggattttttg gatcgctgct
     4081 ccattcgtac cagccgccgt cgtaaacgga gacgttattc cagcccattg ctcgtgcgta
     4141 cataaaggtt tcggacgcgc gccagccggt gccgcagtag aatgaaactt gctgatctgg
     4201 tctgatattc catgctttcc acatagccgt aatatcatcg gcgctgcgca tggtgccatc
     4261 cgggttatga aaatcttcca tatgcgtcga gtcgctacca gcgtgtcccc aacgtgctcc
     4321 ggctatttca ccttttggtt taatatagct gtaaccgctg gtcgtaccga taaactctgg
     4381 ccacgaacga atgctcacca gcgatgcatc ctggcgatgc agcagtccac gcgcctgttc
     4441 catatcaagc attagttgcg gttgtgccgg gatcttcacg ccgaaatccg gttccgcttt
     4501 cactttcggt ggcgtaccgc gctcaacggg cagacccgcg tcggaccagg tttgccagcc
     4561 gccatccagc aggcgcacat ctttcacgcc ggcataaagc ataatctgcg ccacacgcgc
     4621 tgcagcgtat acgtcacgcc cgtacagaat gaccgtggtg tcatggcgaa tgccgtgttt
     4681 tgccagcatc gcttttagtt gttcatcaga aactttgttc cacagcggtt cgctttccac
     4741 ttcgttggta tcgatgtagt cagcaccggg aatatggctg ataaggtaaa gcttaggagc
     4801 gccccaggcc gcttcaatga ctttccagtc tccggcaggt ttctccgtaa cctctttacc
     4861 ttgttgcagg tcgtgcagcc attgcggata aaccagctgc tcaaagtgcg gcagtttttg
     4921 tagacgggaa ggttcgctta gcgcgtcact cagaatggag atatgcgtaa aacctgcttt
     4981 ttgcagtcgc gtattgacgg cgtcgacatc tttgccatta ccgtacagcg ccaccggggc
     5041 atcggctttc aggttatgtt gcttgatcca cgcgtttagc tgttcggtgc tcattttgtc
     5101 aagccagctg gcagagaggt ttaaggcggc aggttcatga ccagaagggc catttaaggt
     5161 ttgtggccag ccgttataaa acgcgctggg gcgagtatct atcgctttgc cattttgttg
     5221 ttgaagctgg tcaagtgtaa gaggcttcgc cagttcagcg gcccacgaag aagcgcaagc
     5281 cagccctaaa gccattgcca gcgcggtcat ttgagaaaca cgtttcatcg aaaatcccga
     5341 cattgtagtt ttgagtgcgc cattctgaac tgacgcattg agaaaaactt tgcgttatcg
     5401 cagtttgttg taattttctg accactgcgc catatccaga acagaactat cagcaggaac
     5461 atcctggaaa tcgtgcgtta cctgaacgac ggggatcgcc agggcgcgaa cttcgctgaa
     5521 cacccactgg cgaaaattat cgcgcagagc cacatcaaga cggctgaatg gctcatccag
     5581 gagtaacgct tttggctggg cgagaagggc gcgaagcaga gcaacgcgcg ctcgctgacc
     5641 gccagacaaa gtggcaggat cctgatggaa agctccatca aggcctgaac gctcaagtgc
     5701 atcattcacg gcattacgtc gggcattccc tttaagtgtc gccggtagcg ccagaagtaa
     5761 attttgcccg acactgaact ggtcaaataa cagtgcatcc tgaaaaagaa tgccaatctg
     5821 acgctgtgcg gtgggtagca tgtcaatccg ttgctcattg agccatagct cacctgtaca
     5881 agaaaactgt ccgggcagtg caccaatcat ccatgaaaac agagtggatt ttccacagcc
     5941 agacggcccc attaacgtga caatgtcacc tttatccacc gtaaagttaa cgtttgtcag
     6001 caagcaactt tctggtaaac gtagcgaaac atttttcacg cagagcatta gcggagtcct
     6061 tgtctgacat aacctaccca ttttgcgact aacgcggtca gggcaaaaat aataagcggt
     6121 aatagcagtt gccataaagc ctgggcggcg agaataccgt tgctgccgcc gctgcttaat
     6181 gccaccgcct cagtggtgag cgtcggaaaa cgccccgcgc ccagccacag tgtcggcata
     6241 tactgcgcaa tactgactga aaatcccacc gcgaaggcaa tcagcgtagg gcgcaacatg
     6301 agtgggcatt tcacgtaaaa gaagattttg gcccgcgacc agcccagtgt ttgtgcaatc
     6361 aaaattaacc gtgaatcaat gcgctgccag gcgggttgca ggataaacag catccacgga
     6421 attacccaca gcagatgccc ccagaccacc gccgtccagc tcctatccag gttcagccat
     6481 agcgccagcg tgtactggcc cgccaccagt ggcagtgccg gtaataaaat gggcaaccat
     6541 agccacaact ggcgacgctg tggcccccat tccagccaca gcagtaacag gagcaaagcg
     6601 atgaatgtcg ccaccagccc cattgtcagg ctgttgatga gcgcttcact attgatcgtc
     6661 gactgatccg cgaggatcgc cagcagaatt acacacagca caccggtcaa gggtaaaaaa
     6721 ctcgctagcg tattgcccgg caataaaggc gtggcaggct tacgaacgcc atctacgcgg
     6781 ggaatagtac gccgccaggt acaccatagc agatagccca gcaaaacgta ggcggcgagt
     6841 aacaacatca gcagcaggct ggcgagtgcg cctttcgttt gttgatcggc gtcgccctga
     6901 gttaaccact gccagctaat tactgccagc gtcggtggat tacctggccc aagaataatc
     6961 gccacatcca cgaccgacag cgaccaggca acaatcgcca gcatcgccat tgccagcgca
     7021 ggcgcgacgg agggcaacag cagccagttc aggcattgcc agcggctgta gcccagcgaa
     7081 tccagcacga tgacttgctg taaaagccgt ttttcactca ataccgctgc taagatccac
     7141 agcagaaatg cgctttcttt caccgccagc gtgaagccca gcccaatacc caatttgtcc
     7201 attggcggag tgaaatacgg gaaatagtca taaagcagcc ctccgtcagc aaagagcaga
     7261 agggcgctgg tggcaaaagc cacatgggga atggcgagca gccacggcag acgggtgcac
     7321 atacgctgcc atttcggccc aggccacagc gccacaatca tcagcagggc aatcaacaac
     7381 gccccgaccg ccgcgatggt tgtcgacacc agcgttgcca gtaatgcctg cggtaactgt
     7441 ggatcggcaa ataacgcctg ccagtgtgtc aacgacaaag caggcgatat cagcgtgaga
     7501 gctgccggga tcagcggtgc ataaatcacc gccaccatcg cccacagcaa aaagattaat
     7561 gcgtaccgta acggtgtagc cattcttgtt ccagcgcatt tacccaacct gcgtgcggtt
     7621 cagccagtac tggcggcaga tcttgcggca ttcttgattg caatgtttcg cgctgcccat
     7681 caggcagttt ttgcggatcg agaacagaag gatcgcccca gacagcggga tctgctttac
     7741 gcaattgcgc atcgggtgag agcaagaaat tggcaactac cttcgccgca gcactggcat
     7801 tcgcgttggc aggaatggtg acgaaatgca cgttgccaat catcccctcg cgaaagccaa
     7861 aactgtaact gcttgcaggc aaatcgccgc tggcgatttt ttgctgcgca tgcgcggggt
     7921 taaaggtcag cgacaggcgc aatgtgccgg atttcagcag agcatccatc cgcgcgggcg
     7981 aaggcgggaa atcctttcct tcgcgccaca aatacggatg cagcgcatca agatattgcc
     8041 acaagggagc agtgacacgg gcgaaagtcg catcgtccgg cgcttctttt aatgcggcgg
     8101 gatcggccgt cagcataatc agcaactgtt caagaaacgc cgtgccggta aagtccggtg
     8161 ggcgcggata ggtaaccgtg ccgggattgg ctttagcaaa ctccagtaag gcttgcggcg
     8221 tttgtggtgg ctgtggcgga acatcgcggc gggcgataaa tgtcagttgt gcgccccccc
     8281 agggcgattc agccccttct gtaggcactg aaaaatcttc ctgcaccggc agctgtgtgt
     8341 cgacatagcg ccagttgggc agagtctctg cccagtcggt ttgcagtaaa ttggcctctt
     8401 ttaaggtgcg gaagttttcg ccgttcaccc agagtagatc caccgagccg cccgttttac
     8461 gtccggctgc ggcttcggtc tgaatgcgct tcaccgcgtc tgcggcatcc gccagacgaa
     8521 caatcttcag gtttatagcg taatgggttt tcatctcgcc gctaacccag tcgagatagc
     8581 ggttaattgc ggtatcgccg ccccaggcgt taaaccagac agtttgccct ttggcctcat
     8641 ttttaatttc ttgccagtca ctggcatgtg ttgccagaga aaacagcgat aacaatccca
     8701 gcaaccaccc acaatggcgc atcatctctc cttaagaacg tttttccgga atacggcaca
     8761 gcccctgagc gggaatgtcc atggcagaat taaaacgggc tgacagattt tgcaggccga
     8821 tcaatgccgt cagttcggtg agcgcctgag cgtcaaaatg cgcagccagt cgggtacgca
     8881 gggcatcatc gaccgttggc ggcgttacgc ttgcggcttc ggcgtactcc agcgccagcc
     8941 gttcttcatc gctaaagagc gggctttgcc gccagtcagc cactgccagc agtttatcgc
     9001 tgctgccggt gcgctcggcg actttcatac tggtgatatc cacacaaaac tcacacaggc
     9061 acatttgcgc aatgcgcgcg ctgacaagcg atcgtactac cggatcgagc ggtgagcgtt
     9121 tgcgctccag ccagccaaca aacatcgaca ccagataaaa gataaacggg atccgccccc
     9181 accagcgaat ggggcttagc acctcgccgt aatggcgacg atgcagccac gcctgcggac
     9241 gtaaaatgaa aggaattttg ctaagcggcg gtaaacccat caggcattcc tcttctggcg
     9301 ctcaagccat attttcttcg ccataaaaat caccacggtc agggcaaaca gaataaacag
     9361 tccggtgaca aaccagaagg taccccctgt taacatgctg cccgcccagg aataaacaat
     9421 agtcgccggt aattgaccaa gcccggtggc gataaaaaac gagcgaaaac gtattgaagt
     9481 caaaccggca gcatagctga ttggatcgaa agggacaaaa ggcaataacc gacagaccag
     9541 gatagtgtgt ttgccgtagc gagtgaaaaa gccgtccata ctgtcaagta cggttttgcc
     9601 ggttaatttt tccaccactt cgcggcccat cactctggcg ataaaaaagc acagcgccgc
     9661 gccggccatc gaactggtcc acgacagcaa gccgccccag aacgcgccaa atagcgacgc
     9721 attggcaaag gtgatcaaaa acgcaggtag cggtgcagca atggcctgta aaatcatcaa
     9781 caagaacgag acaactgccg ccagtgcgcc gtaagactga ataaagcgtt ctataccctg
     9841 ttggtccacg gcggcaaacg ccgcaacgct gcgattgata aactcatgga cgccgggaag
     9901 cagcgcccat gccagcagca tagcgaacaa caggatgatg agggttatac ggtagtacca
     9961 gatttttcgc gattgcatct tcatcatcct ttatcctggc tttattcggt gattttaaag
    10021 ataaccgttg ccagcgtgtt atccgccggg agaaccgagg cattgccttt gaatttaact
    10081 tcacctcgtt tttcaaccgc accataagtg tatgttgcat tgctgacgat gccgaccggg
    10141 cagctatcca gacacaccag gcaacccgtt ttcttctctt ctgctgccgt taaattaccg
    10201 ccaaagcgca tgtccagttt cttgccatta ctgtcaacaa tcacttcatc gaaggaatac
    10261 gtttttgccg ccccttgcca gttgaccgaa atatccagtt tgctgcctgt gacatgagtc
    10321 gtttctttat tatccatcgt catgttttcg cccggtgtac cacctgcctc tttcagggct
    10381 tcataaaatg ctttcggggt cgcataaccc ataaacagcg atttatgtcc gttggagcca
    10441 tctttaaaca caataccgtg acgagtgtcg tcggtgagga aacgaccatt aatttgcacc
    10501 agcatggtta ccgtatggtt attggcatcg acgcgcagcg gttggctgtt cgaaagtccg
    10561 tcgtattcca cttttgccgc cgcattctct ttttgatcgc agccgctaag cccagcaaca
    10621 gccagtaagc agagtgcagt cagtcctttt ttccatgaca ctgaataatg ttgcaacatc
    10681 gttctatcct tcattttttg ggtgagtaag tgggttgcgg cgcgaagcag acagatccac
    10741 atgtttgtgt cgggcgtaga gtttggcgag ctggacgaga ataaacagcg ccaggcccgc
    10801 cagacagagt tgtaaaataa agcgcagcgt aatgccttca ttggcaagat cgcttgccat
    10861 cacggtataa attacgattc cgggcagggt cgtgagtgcc gaaataaggg tatagggcca
    10921 gaaagcaata gcggttaatc cgtaagcgta attttgaata ttgtacggaa acagcggaat
    10981 taagcgggtc agaataagaa aatcgatgcc gttacgcgca atgccttttt ctatagcctt
    11041 gaaggtatgg ctatggccca cgtatttcag cagtaaatca cgtcccatcc agcgcgccag
    11101 caggaatgag cacgaggagg ccagcgtagc ggcaattaat gagagtagtg tccctaagag
    11161 cgggccaaaa actattcctc ccgcgatcac cagtatgctt cctggtaaca gaaagagggt
    11221 ggcaatgatg aataacagaa tatagaggct ataaccgaac aatccgctct ggcgaatgag
    11281 tgtctgtaag tggggtaaat cggtgagcag atcgaataaa ccgaaagcgt ggatagcgta
    11341 aatgaccagc gcaaaaataa gacaggcaaa cagaaattta cgctcagcgt tcatcgtgga
    11401 gtttcctata tcaaacttga taatagagaa gtggtcagac aactaaaaac gcagtgacgg
    11461 tataacaaag gacggcaggc aacaaatcat tactgacagt ttgttgattt ttctcaaggt
    11521 taattctcct gacccagttt gagccaggag agctgctaaa ttagcggcgg aaggtcgccc
    11581 agacgggggc gtgatcggac ggtttttcca tgctgcggat ttcatagtcg atgccggttt
    11641 ctacgcaaca ttctgccagc ggttggctgg cgagcagcag gtcgatgcgc agaccacggt
    11701 tatcgtcaaa accttttgag cggtaatcaa accatgagaa acgatctgct gtttgcggat
    11761 tcgcatggcg gaaggtatcg accaaccccc agctcatcag cctgtccatc cattcgcgct
    11821 cttccggcag gaaagagcat ttaccggtac gcagccagcg cttacggttc tcttcgccaa
    11881 tgccaatatc caggtcgcca ggactgatat tcatatcgcc cataatcagc accggatttt
    11941 cacgcttgag ttcggtttcc aggtagtttt gcaggttctg ataaaactgc gcttttgccg
    12001 ggaattttat cggatggtcg cggctttcac cctgcgggaa gtaaccgttg atcacggtga
    12061 cattacccag cggtgagggg atttccgcca taataatccg ccgttgcgcc tcttcgtcgt
    12121 caccgggaaa gccgcgacgc acggcaatcg gcgtctcttt ggtcagcagc gccacgccat
    12181 aatggccttt ctgcccgtga taaaacacgt tgtagccgag cttcgccacc tcttcgaggg
    12241 gaaacatatc gtcatgaact tttgtctcct gcaggccaat cacatccggt tggtgctttt
    12301 cgacgatggc ttcaagctgg tgaggtctgg cgcgcaggcc gttgatatta aaagagacaa
    12361 atttcatagt cgctgccatt tattcagata aagagtgcgt ggatggtagc agaatttcgc
    12421 tttgcttacc gcctgttgtt aagtgatttg atcgatggtt gttaatgatg tcaacgatgg
    12481 cgcaaaaaat gcccgctttt ggtgcgcgtt gcgtcagaat ggcgcagtaa tttccagtaa
    12541 atttcgtcaa tgatcacaat tccagaatta tattttactt ctgcataaca ttgcgttttt
    12601 tattcttttt attcacccac atcgcaaacg tattcacttt atatgcactt taaatgcata
    12661 tggtttggtt ataactcctt gatttccagt tagcctccgc ggtttatgca cttttatcac
    12721 tggctggcac gaaccctgca a
//
LOCUS       DAZKFU020000131        12577 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZKFU020000131 DAZKFU020000000
VERSION     DAZKFU020000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.132"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..161
                     /locus_tag="ACHOQQ_RS14990"
                     /old_locus_tag="pgaptmp_002998"
     CDS             <1..161
                     /locus_tag="ACHOQQ_RS14990"
                     /old_locus_tag="pgaptmp_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HGU4471217.1"
                     /translation="GFITPDDGSKDVFVHFTAIQSNEFRTLNENQKVEFSIEQGQRGP
                     AAANVVTL"
     gene            172..360
                     /gene="ymcF"
                     /locus_tag="ACHOQQ_RS14995"
                     /old_locus_tag="pgaptmp_002999"
     CDS             172..360
                     /gene="ymcF"
                     /locus_tag="ACHOQQ_RS14995"
                     /old_locus_tag="pgaptmp_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4471218.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            335..565
                     /gene="ymcE"
                     /locus_tag="ACHOQQ_RS15000"
                     /old_locus_tag="pgaptmp_003000"
     CDS             335..565
                     /gene="ymcE"
                     /locus_tag="ACHOQQ_RS15000"
                     /old_locus_tag="pgaptmp_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGU4471219.1"
                     /translation="MHRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            555..728
                     /gene="gnsA"
                     /locus_tag="ACHOQQ_RS15005"
                     /old_locus_tag="pgaptmp_003001"
     CDS             555..728
                     /gene="gnsA"
                     /locus_tag="ACHOQQ_RS15005"
                     /old_locus_tag="pgaptmp_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU4471220.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(776..1849)
                     /gene="yccM"
                     /locus_tag="ACHOQQ_RS15010"
                     /old_locus_tag="pgaptmp_003002"
     CDS             complement(776..1849)
                     /gene="yccM"
                     /locus_tag="ACHOQQ_RS15010"
                     /old_locus_tag="pgaptmp_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4471221.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRYMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1921..4665)
                     /gene="torS"
                     /locus_tag="ACHOQQ_RS15015"
                     /old_locus_tag="pgaptmp_003003"
     CDS             complement(1921..4665)
                     /gene="torS"
                     /locus_tag="ACHOQQ_RS15015"
                     /old_locus_tag="pgaptmp_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGU4471222.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTLEVGSCFCLRLPLRVATAPAPKTANQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLEHYL
                     QLQVNNDIPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            4748..5776
                     /gene="torT"
                     /locus_tag="ACHOQQ_RS15020"
                     /old_locus_tag="pgaptmp_003004"
     CDS             4748..5776
                     /gene="torT"
                     /locus_tag="ACHOQQ_RS15020"
                     /old_locus_tag="pgaptmp_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGU4471223.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQSSTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5749..6441)
                     /gene="torR"
                     /locus_tag="ACHOQQ_RS15025"
                     /old_locus_tag="pgaptmp_003005"
     CDS             complement(5749..6441)
                     /gene="torR"
                     /locus_tag="ACHOQQ_RS15025"
                     /old_locus_tag="pgaptmp_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGU4471224.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQVQPLTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6571..7743
                     /gene="torC"
                     /locus_tag="ACHOQQ_RS15030"
                     /old_locus_tag="pgaptmp_003006"
     CDS             6571..7743
                     /gene="torC"
                     /locus_tag="ACHOQQ_RS15030"
                     /old_locus_tag="pgaptmp_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGU4471225.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDKLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7743..10289
                     /gene="torA"
                     /locus_tag="ACHOQQ_RS15035"
                     /old_locus_tag="pgaptmp_003007"
     CDS             7743..10289
                     /gene="torA"
                     /locus_tag="ACHOQQ_RS15035"
                     /old_locus_tag="pgaptmp_003007"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HGU4471226.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSKNLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTIAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVDQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10286..10885
                     /gene="torD"
                     /locus_tag="ACHOQQ_RS15040"
                     /old_locus_tag="pgaptmp_003008"
     CDS             10286..10885
                     /gene="torD"
                     /locus_tag="ACHOQQ_RS15040"
                     /old_locus_tag="pgaptmp_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGU4471227.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAMDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(10978..11283)
                     /gene="cbpM"
                     /locus_tag="ACHOQQ_RS15045"
                     /old_locus_tag="pgaptmp_003009"
     CDS             complement(10978..11283)
                     /gene="cbpM"
                     /locus_tag="ACHOQQ_RS15045"
                     /old_locus_tag="pgaptmp_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGU4471228.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11283..12203)
                     /gene="cbpA"
                     /locus_tag="ACHOQQ_RS15050"
                     /old_locus_tag="pgaptmp_003010"
     CDS             complement(11283..12203)
                     /gene="cbpA"
                     /locus_tag="ACHOQQ_RS15050"
                     /old_locus_tag="pgaptmp_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGU4471229.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12463..>12577
                     /locus_tag="ACHOQQ_RS15055"
                     /old_locus_tag="pgaptmp_003011"
     CDS             12463..>12577
                     /locus_tag="ACHOQQ_RS15055"
                     /old_locus_tag="pgaptmp_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471230.1"
                     /translation="MGNIHGISCTANNYLKQAWNDIKNEYEKNKTYSITLFE"
BASE COUNT         3035 a         3223 c         3313 g         3006 t
ORIGIN      
        1 ttggctttat cactcctgat gatggcagca aagacgtttt cgtccatttc accgccatcc
       61 agagcaatga attccgcacg ctgaacgaaa atcagaaagt tgaattttct attgagcagg
      121 ggcaacgtgg ccccgcggca gcgaacgtag ttacgctcta aggttgccgt tattactcaa
      181 catctccatt ttcgctgtcc atgttgtcat ggttcacagt accgcacatc ggcattcgat
      241 gtgacggagc gaaacccttt gggcgctaag tgtatttttt gtaaatcgac gatgatcacc
      301 tttgataacg tcgcgctaca aatacgcact gaccatgcac cgctggattt cacaaaataa
      361 tatcaggctc cctcgtggag ccttttttat atctgcctta tttttcttca acgctgtatg
      421 tatagtaagc gataacctgt tgattattga atctttcggg gagatggctt ataacatttc
      481 ttacctgacc agggtaccgg gaaccaacac cttactggcg tgttgctgtc tttcaagacc
      541 agacgaggtt aacagtgaat attgaagagt taaaaaaaca agccgaaacg gaaatcgccg
      601 actttatcgc gcaaaaaatc gccgagctga acaagaatac agggaaagaa gtctctgaaa
      661 ttcgcttcac cgcacgagaa aaaatgaccg ggcttgaaag ttatgatgtc aaaatcaaaa
      721 taatgtgatt ttgtgaacat cacccgtgcg aggtgatgtt ccgcttgttg ctaatttagt
      781 gaccaatcat tggcgcttgc ggaattaagc gtcggtacaa ttcctccggc accgggcttt
      841 gccatactcc cgcatacatt gcgtaaccaa tcaccgcaaa cataatcccc agaaccagta
      901 gcgtcattaa ccagccagac aacgcaaagg cttttttatt tgccgcaggt ttttgcagtg
      961 aaaaggtcaa tgttgaggct accggacatg actctacgca agtcatacag ccggtacatt
     1021 ccactgttcg tacctgaatt aatttatcga ccgggatccg tgatgggcaa ttttttgcgc
     1081 atttgccaca gtcgatacaa ctttcggcat tgcgacgaat cttaaacggc gacaatagcg
     1141 aaaccacgcc catcagcgcg ccatatgggc aaagataacg acaccaggca tggcgaataa
     1201 acaggctggc aatcagcaaa acggtcacgc tgattaatgt cgcggtcccc atataacgaa
     1261 agaaatcgag cattttaacg tccatcacca cgctgtaggg cgacaacata aaatagtgaa
     1321 tcgcctgagc gggcatcaat aacgcgatat agagaaaaaa actcaacagc aaatacttta
     1381 caccgcgcag aggaatatcc agccagcggg gaaggacaaa ttgccgacca aaaagtttgt
     1441 taccgagagc gccgattaat tcagaaagcg taccaaccgg gcataaccat gaacaaaagg
     1501 cctttttgag taatagactg atgacgataa aagcgaccaa taacagcatt gcggcggcgt
     1561 ggacggacgg taactgacct gttgcaaggc tatatttcag attcatcaga ccggcaatcg
     1621 gtagccagcc ttcgatacct cccggtctgg cgacaaatgt cgtactacct gccgtttcgt
     1681 aatagcgcac ccaataccag aacgtgatgg caatataaat attcattgcc aatagtaaga
     1741 attgcgtcgc tttacgccag gtcgtggcat tgcgccagtc attccacggt aatttgccgc
     1801 ccgtcgtgcc tggccgccgc tgccagcggg ttcttttttt ctctgccatg attttgccag
     1861 tccgttaagt tgcataccaa atgccactat tctagttatt cttaactggc tgatattgat
     1921 tcaaatcgcg ttcaggtctt tcttattcaa ccatgcttcc agagcggcaa cactgcgtgt
     1981 aatttcttcg tgtggaaggg gggcagataa tggctgctgc tccagttgtg cgcatagctg
     2041 gctggcgctg cgcattccca gactcgagca actgcttttt agctgatgcg cggcacgctt
     2101 tattttctcg ctgtcctggc tggcgcgggc aatgtcgatt tcatcgagaa gcggcagggc
     2161 atgttgttta aataatgcca gccattcgtg gatcttctcc gtccccatta attgagcatc
     2221 ttcattgagt tgcgatacat caagtggtat atcgttattg acttgcagtt ggagatagtg
     2281 ctccagtaac tgaccgagca cttcacgcgg caccggttta gggataatcc cgcgaaatag
     2341 tgaactggta cgctggcgca gcgtttcgtc aatgacatga gcgctaaagc caatcaaaac
     2401 cagcgacgga tattgctgtg ccagttgccg ggcaagggta atgccgtcga tatccggcag
     2461 atcaaaatcc accagtgcgg cagcaaacgg ttcgctattt tgcagtgtct ctaaagcctg
     2521 cgcggcattg ccaatagcaa caacctgcgc accactggtg ttcagcatct caacggtaat
     2581 tcgctgggtt agcgggttat cttcaattaa cagcaaacgt aaaccgtcca gacgcaccgc
     2641 ctgattggct gtttttggcg cgggtgctgt ggcaacacgt aacggcaagc gtaaacaaaa
     2701 acagcttcca acctccagcg tgctggtggc gctcagttcg ccgcccatcg cctgggccag
     2761 acggctactg atagtcagcc ccagcccggt gccgccgcgt ttgccactta cctgaataaa
     2821 tggctggaag atttctgcca gtttcgcggg atcaataccg cagccgctgt cttccacttc
     2881 gaccagccat tgctcaccat cagtacggct acgtaaaacg atctgccctt cgtcagtaaa
     2941 acgcagggcg ttgctcaaca ggttggtaat gacctgacga atacgccgcg gatcgcccat
     3001 taacgcagtc ggtacatcgt cggcaattgc cgttgccagg cgaatcgggc gacctttcac
     3061 ccgtccactc attaattgca gggtactttc cagcagcggt cgcggttcaa agggttcatc
     3121 gctgaccgaa acattcttgc cgcctgcttc gatagccgaa taatcgagaa tatcgttcag
     3181 gatggtcagc aacgattcgc cagagtcggt aatcgcccgc aaatcatcac gctgggcgtt
     3241 aagtgcgggg ttatctgcca gtagctgtgc ggtgccgaga ataccgtaca gcggtgtgcg
     3301 gatctcatgg ctcatcgccg ccagaaacgc cgattttgcc tggctggctt tttctgcttc
     3361 cgcccgtgcc tgtcggtgtt ctatcaccag ttcctgcaat tcagctgtac gtgctttgac
     3421 ctgcgccgcc agctgttcgc ggtggcgatt cagtgcatga acactgctgc gaaacgcatc
     3481 catcagccgc ccgatggtat ccagctcccg tacgccagcg gtttccggaa aaggggagtc
     3541 aatatcaccg tccagcagcc gttgcagcgc ctgcgtttgt tcggcaagtg gacgcgtgac
     3601 tgagcgataa accacgcgcc agaggatcag aatcagtgcg caaagtgaaa ccatccccag
     3661 caataacagg ctgtattgcc cgcgtgcact ggctttttcc agatgcgcca gtccatgctg
     3721 attgcgcagc tcaatggtgt cgaccagctg actgacttcg ctactaaact gcgcgaactg
     3781 ggcgatatta ttttgggcga gagtttgcag gtgattgctg atttcactgt cctgctggaa
     3841 cagcgccagc aaatcgctat attggctaac ggtagttaac gttgttgcga cctgagcacg
     3901 aactcccgga tcttcaatgc gtatttgccg acgttgcaga attttcaccg cattattgag
     3961 ctgcttttcc agcgttggcg catttttctg gatctgctcc agccccagat tcatcaccat
     4021 ttgctgcacc cgcagagcgc taaggcgcag ttcattcatc tggttaacat actcaagatc
     4081 gatatcaatc agccgatcga gtgcactttc agcagcctga cgctgatctt gttcgatcaa
     4141 atcgtaaatc ccggcctggg tcgctccggc ggaggttgcc gcattattcg cctgaccttg
     4201 cgccaggcgt gcgatctcat cggcggcagc gactatctgc tgactgagtc gctgttgttg
     4261 ctggcgcagt tgcaaacgct gccccaccag ttccccttgc tgacgtaacg aacgggagat
     4321 ctcctgctcc tgttgttcaa tagcggtggt atcaaaacct tgttcccgta acgcttgcag
     4381 caacgcatta atcttcaggc tttgtgcggt gagcattcgc ccctgcgcct gccacatctt
     4441 ttcgttatcg gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct
     4501 caactggcgc gccatattca tggtaggaat caatgcctga gtattgtctt tttccacctg
     4561 gctgataaag cgcaggttgt accatcccac cagggtactg gtcagggtta acagcgccat
     4621 cagggcaaag cccatccaga gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa
     4681 ggtgaaaaat gttgagtcgc aaagaggaat gcatctagca taaagcctta ttattgatga
     4741 ggctatcatg cgcgtactgc tatttttact tctttccctt ttcatgttgc cggcattttc
     4801 ggctgataac ctgttgcgct ggcatgatgc gcagcatttc acggtgcaat cctctacgcc
     4861 gcttaaagcc aaacgcgcct ggaaactgtg cgcgctttat cccagcctga aagattcata
     4921 ctggttatcg ttgaactatg gtatgcagga ggctgctcgc cgctacggtg tggatttaaa
     4981 agtgctggag gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg
     5041 taaacagtgg ggcgcagagg ccattttgct cggtagtagc acgacgtcat ttcccgatct
     5101 gcaaaagcag gtagcaagtc tgccggtgat cgaactggta aatgctattg atgctcccca
     5161 ggtgaaaagc cgcgttggtg taccctggtt tcagatgggc tatcaacctg ggcgatattt
     5221 ggtgcaatgg gcgcacggta aaccactgaa cgtgctgttg atgcccggac ccgataacgc
     5281 cgggggcagt aaggagatgg tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg
     5341 tattgttgat attgcgcttg gtgataacga tattgaaatc cagcgtaacc tgttgcagga
     5401 gatgctggaa cgccatccag aaatcgacgt cgttgccgga acggccattg cggcagaggc
     5461 ggcaatgggg gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt tttatctttc
     5521 acatcaggtg tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcaaat
     5581 ggtctggcag ggggaactgg cagttgagca ggccatcagg caattacagg ggcaatcggt
     5641 gtctgataat gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgcgaaca
     5701 tatccgccgc tcgctgtcgc cagggggatt tcgtccggtc tatttttatc agcacacatc
     5761 agcggctaag aaataacctt cgccatgttg cgtcaccagt aaatccgcgc tgagtttatg
     5821 gcgtaaacga cgaattaaca catcgacggt gcgcaggtca gggttttcca cccgacgcgc
     5881 agagagcata cgaagcagac gttcacggct gagaatttct cccggattgg tcacaaatgc
     5941 caccaacatt tcatactctg cgcgggtcag tttaatcggc tcaccatccc gctccagcgt
     6001 atggcgcgac acattcaggc aataacccgc aaagcgatag cagttgtcct gagtgagcgg
     6061 ttgaacttgt cgcgcgaggt cgattcgcca gagcagattt tttacccgta ctaccagttc
     6121 gcgcagttcc agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg
     6181 gtcaatccga tcgctgcgtc cggtaaccag aataatcccc accgttgagc gttctcgcag
     6241 ggcgcgggtt aacatcaggc cattttcatc gggcaagttg atatccagca gaattaaatc
     6301 caccggctga ttctgcataa tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt
     6361 atacccctcc tgagtgaagt aggattgtaa tcgcgcctgg gtaaccggct catcttcaac
     6421 aataacaatg tgatgtggca tcagagggtt ttactcattc tgttcatatc tgttcatatt
     6481 ctgccgtaag ccattcatcc tgaccagtgc cgctgttcat atttgctcat taagatcgct
     6541 tcactaaacc ataattttac aggggctatt atgcggaaac tctggaacgc gctacgccga
     6601 cccagtgctc gttggtcggt actggcgctg gtcgctattg ggattgtgat tggcattgcg
     6661 ctgattgtat tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt
     6721 tgccacagta tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc
     6781 tccggcgtgc gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg
     6841 aagcgtaaac tggaagcgag caacgatatc taccagacct ttattgccca ctccattgat
     6901 acacctgaaa aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcacgaatg
     6961 aaagaaaaca actcggcaac ctgccgctcc tgccataact acgacgcgat ggatcatgcg
     7021 aagcagcatc ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc
     7081 atcgactgtc ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag
     7141 cagttcgatg agctgcgcgc cggtgctaat gacagtggcg acaagctgta ctctattgat
     7201 attaagccga tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgcc
     7261 tcggaagtga aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg
     7321 acagaaagcg ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt
     7381 gcctcgattc gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac caccgtagcc
     7441 gacaccaata ccgagtggag caagttgcag gccaccgcgt ggatgaagaa aggcgacatg
     7501 gtgaacgata tcaaaccgat ctgggcttat gcggattcgc tgtacaacgg cacctgtaac
     7561 cagtgccacg gcgcaccgga aatctcccac tttgacgcta acggttggat cggcacgctc
     7621 aacggcatga ttggctttac cagcctcgat aagcgtgaag aacgcacctt gttgaaatat
     7681 ctgcaaatga atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa
     7741 taatgaacaa taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg
     7801 gcttaaccgt cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcgactgcgg
     7861 cgcaagcggc gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg
     7921 gggctatccg cgcgacggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg
     7981 ataaatatcc gtcgaaaatg attgccggat tgccggatca tgtacacaac gcggcgcgta
     8041 ttcgttatcc gatggtacgc gtggactggc tgcgtaagcg ccatctgagc gacacctccc
     8101 agcgcggtga taaccgtttt gtgcgtgtga gctgggatga agccctcgac atgttctatg
     8161 aagaactgga acgcgtacag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt
     8221 ggcaatcgac ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc
     8281 atggtaatag cgttggtacg ggcggagatt actctactgg tgctgcgcag gtgatcctgc
     8341 cgcgcgtagt cggttcgatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc
     8401 agaacagcaa aaccattgtg ctgtggggct ccgatttgct gaaaaaccag caagcgaact
     8461 ggtggtgccc ggatcacgat gtttatgaat attacgcgca gttgaaagcg aaagtcgccg
     8521 ccggtgaaat tgaggtcatt agcatcgatc cggttgttac atccacccat gagtatctgg
     8581 ggcgcgagca tgtgaagcac attgcggtta acccgcaaac tgacgtgccg ctgcaactgg
     8641 cgctggcgta tacgctgtac agtaaaaacc tgtacgataa aaacttcctc gctaactact
     8701 gtgtgggttt tgagcagttc ctgccgtatc tgctgggtga gaaagacggt cagccgaaag
     8761 atgccgcatg ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt ggactggcgc
     8821 ggcagatggc ggcgaacaga acgcagatta ttgctggctg gtgcgtacag cgtatgcagc
     8881 acggtgaaca gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc
     8941 tgccaggtgg tggctttggt tttggctggc actataacgg cgcaggcacg ccggggcgta
     9001 aaggcgttat tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca
     9061 gtgattacaa aggctacagc agcaccattc cgattgcccg ttttatcgat gcgatcctcg
     9121 aaccggggaa agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt
     9181 gtatttttgc cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag
     9241 gctggcgcaa gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct
     9301 ttgccgatat cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg
     9361 gcaaccactc caaccgtggc attatcgcca tgaaacaggt cgtgccgccg cagttcgagg
     9421 cgcgcaacga ctttgatatt ttccgcgagc tgtgccgccg ctttaatcgc gaagaagcct
     9481 ttaccgaagg gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgttcagc
     9541 aaggcaaagg acgcggcgtt catttgccag cgtttgatga cttctggaat aacaaagagt
     9601 acgtcgagtt tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg
     9661 atctcgaacc gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg
     9721 atatgaacta cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc
     9781 acggtgggcc cggctcgcag acgtatccgt tgcatctgca atctgtgcat ccggatttcc
     9841 gacttcactc gcagttatgt gagtcggaaa ctctgcgtca gcaatatacg atagcgggta
     9901 aagagccagt gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg
     9961 tggtacgcgt ctttaacgct cgcggtcagg tgttggcagg ggcagtagtt tctgatcgct
    10021 atgcacccgg cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg
    10081 agcctggtgc gctgtgcaaa tacggtaacc ccaacgtgtt gacaatcgac atcggtactt
    10141 cgcagctggc gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaacg
    10201 gaacagtgga tcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat
    10261 atgttcccgc gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt
    10321 ttacgcctgg ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat
    10381 cgccagtgcg cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc
    10441 ggcgatggat gagctggaaa accgtgttgc cactctgaca gtacgtgacg atgcccgtct
    10501 ggaactggcc gctgactttt gcggcctgtt tctgatgact gacaaacaag cggcgctgcc
    10561 gtatgcatcg gcctacaaac aggacgagca agaaattaaa cgcttgttag ttgaggcagg
    10621 gatggaaacc agcggcaatt tcaacgaacc ggcagatcat ctggcgatct atctcgagtt
    10681 gttgagtcat ctgcattttt cgctgggaga ggggacggtt cctgcgcgaa gaatcgacag
    10741 tttacggcaa aaaacactca cggcgctgcg gcaatggtta ccagagtttg ttgcgcgttg
    10801 ccatcagtat gacagctttg gtttttacgc ggcactaagc cagttattgc tggtgttagt
    10861 tgagggcgac caccaaaaca gataacgtcg tttgttggcc tgataagacg cgtttagcgt
    10921 cgcatcaggc attatggggc agttgccgga tgcgtcatca catccggcaa acacacatca
    10981 cggatgagct acaaaccggg aaagccgctg gcgcagcagg cggttttcct gcttcaggtg
    11041 cgcaatatca tccattaacg tcagcgccac cgcgatcccc ggccagtcca gagccagttc
    11101 atgacgcagg cgcaccgcgc gttgcaccac gatggctgca tggtcgtcaa atacccaggt
    11161 tgtttcctgg atctcacgcg gttcaaccac ccccaaaccg acaatttcat tcagttcctc
    11221 ttcagagatg ccggtatgca ggcaaaattc ggtaatagta aaagtcaccg taacattagc
    11281 cattatgctt tcccccaatc tttacgtgga tcaaaagacg actgggcgtc tgccagttgc
    11341 tgccacaacg cggcagtgtt ttcatccggt ttcggcggca tcacgatttt cagtaccgca
    11401 tacagatcgc cggtctgttt tttgctcacc agacctttgc ctttaacgcg caatcgttgc
    11461 ccggcctggc tgccaggcgg gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg
    11521 actttagcac ccagcgccgc ttcccacggg ctaaccggca ccacaatttc cagatcctgg
    11581 ccgacaatat caaacagcgg atgtggcgca atatgaatca ccagccacaa atcgccattt
    11641 ggaccgccgt tttcgcccgg cgtcccctgg cctttcaggc ggatgcgttg accattgccg
    11701 acgcctgccg ggatcttcac attcagcgtt ttcgggattt cctgttcgat catgccaaag
    11761 gcgttataaa ccggcaggtt atagctgatg gtacgcttat gctcagtaag cgtttcttcg
    11821 aggaataccg ccacttcgat ttcaatatcg tggccgcgtg tggcggggcg ttgacggctc
    11881 tggcgggcat gctgaccgaa aattgacgag aagatatcgt caaaatcttc ggcgttaaaa
    11941 ctctgaccgt cgccatggtg gaactgacgg ttaaattgcg gatcgttgcg atgttgccac
    12001 atctgatcat actcagcgcg acgttgttca tcacttaaca cttcccaggc ttcagcgacc
    12061 tctttgaagc gggcttcggc atccggttct ttgctgacat caggatggta tttgcgggca
    12121 agtcgacgat aggcggtctt gattgtcttg agatcgtccg tcggtttcac gcccatgatg
    12181 gcgtaataat cctttaattc catagcgtta tctcgcgtaa atcaacacaa attgaaggaa
    12241 cccctgtaag gtaactccta taagtgtagg gtaatcctca aaatttcata tgccaacaca
    12301 gaatatgtta ttgaaatcat cgcggagagg aggtcgcctc aaggtggggt gctgaacata
    12361 ttttaaacag gtgaaaaagg gtgagcgatt tttgatagtt gaaacaggca ctttaagttt
    12421 tactagtgcg tcattattta ttaaatttta taggcgctat atatgggtaa tatacatgga
    12481 attagttgca ctgcaaataa ttatttgaag caagcctgga acgatataaa aaatgagtac
    12541 gaaaaaaata aaacatattc aatcacgctt tttgaaa
//
LOCUS       DAZKFU020000132        12480 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZKFU020000132 DAZKFU020000000
VERSION     DAZKFU020000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.133"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(31..1314)
                     /locus_tag="ACHOQQ_RS15060"
                     /old_locus_tag="pgaptmp_003012"
     CDS             complement(31..1314)
                     /locus_tag="ACHOQQ_RS15060"
                     /old_locus_tag="pgaptmp_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGU4471231.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1427..3361)
                     /gene="yeaG"
                     /locus_tag="ACHOQQ_RS15065"
                     /old_locus_tag="pgaptmp_003013"
     CDS             complement(1427..3361)
                     /gene="yeaG"
                     /locus_tag="ACHOQQ_RS15065"
                     /old_locus_tag="pgaptmp_003013"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGU4471232.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3797..4543
                     /gene="mipA"
                     /locus_tag="ACHOQQ_RS15070"
                     /old_locus_tag="pgaptmp_003014"
     CDS             3797..4543
                     /gene="mipA"
                     /locus_tag="ACHOQQ_RS15070"
                     /old_locus_tag="pgaptmp_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGU4471233.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4633..5487
                     /gene="yeaE"
                     /locus_tag="ACHOQQ_RS15075"
                     /old_locus_tag="pgaptmp_003015"
     CDS             4633..5487
                     /gene="yeaE"
                     /locus_tag="ACHOQQ_RS15075"
                     /old_locus_tag="pgaptmp_003015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGU4471234.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASKCKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLTGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5538..6422)
                     /locus_tag="ACHOQQ_RS15080"
                     /old_locus_tag="pgaptmp_003016"
     CDS             complement(5538..6422)
                     /locus_tag="ACHOQQ_RS15080"
                     /old_locus_tag="pgaptmp_003016"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGU4471235.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6506..7501)
                     /gene="gapA"
                     /locus_tag="ACHOQQ_RS15085"
                     /old_locus_tag="pgaptmp_003017"
     CDS             complement(6506..7501)
                     /gene="gapA"
                     /locus_tag="ACHOQQ_RS15085"
                     /old_locus_tag="pgaptmp_003017"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4471236.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7843..8256
                     /gene="msrB"
                     /locus_tag="ACHOQQ_RS15090"
                     /old_locus_tag="pgaptmp_003018"
     CDS             7843..8256
                     /gene="msrB"
                     /locus_tag="ACHOQQ_RS15090"
                     /old_locus_tag="pgaptmp_003018"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGU4471237.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8298..8570
                     /locus_tag="ACHOQQ_RS15095"
                     /old_locus_tag="pgaptmp_003019"
     CDS             8298..8570
                     /locus_tag="ACHOQQ_RS15095"
                     /old_locus_tag="pgaptmp_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGU4471238.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            8940..10016
                     /locus_tag="ACHOQQ_RS15100"
                     /old_locus_tag="pgaptmp_003020"
     CDS             8940..10016
                     /locus_tag="ACHOQQ_RS15100"
                     /old_locus_tag="pgaptmp_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4471239.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPMCGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10043..11422
                     /locus_tag="ACHOQQ_RS15105"
                     /old_locus_tag="pgaptmp_003021"
     CDS             10043..11422
                     /locus_tag="ACHOQQ_RS15105"
                     /old_locus_tag="pgaptmp_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4471240.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIHQTGKPLPPVVIADDGKTPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11439..>12480
                     /locus_tag="ACHOQQ_RS15110"
                     /old_locus_tag="pgaptmp_003022"
     CDS             11439..>12480
                     /locus_tag="ACHOQQ_RS15110"
                     /old_locus_tag="pgaptmp_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HGU4471241.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
BASE COUNT         3116 a         2928 c         3163 g         3273 t
ORIGIN      
        1 agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat tttgtttatg
       61 aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct gcatcgcaaa
      121 gttgtcgaaa gtagattgca gatgctcata ttctcgccac agtgtctgat gcgcgcgacg
      181 ggtaatttcg atatagctgt aataacgaac aacgggtaat aattttttcg ccagaatttc
      241 atggcaaagc ggagagtcat cggcccagtt atcgccgtcc gatgcttgtg cagcgtaaat
      301 attccactgt gccgggttat aacgctcttt cactacctca tccatcagtt tcagggcgct
      361 ggaaacaatg gtgccgccag tttcctgcga gtagaaaaac tcatgttcat cgacttcttt
      421 cgcctgggta tgatggcgga tgtatacgac ttccacgttc ttatacgttc tgctgaggaa
      481 cagatacagc agaatataaa aacgcttagc catatcttta gtggattgat ccattgaacc
      541 ggaaacgtcc atcaggcaaa acatcactgc ctggctggag ggatcgggcc gcttctcgta
      601 gttcttgtaa cgtaaatcga aggtgtcaat aaaagggacg cgttcaattt tggcacgtaa
      661 ttctgcaatt tctttgcgca gacgttcctc ttccagcagc tgcgcaggtt cactgttgct
      721 gatgatggcc aaattctctt ccagtgcatg aagttcccgc cgcttgcctg ccgtcatggc
      781 tgtgcgtcgc gccagtgagt tctgcaagga acgcacaacg ctgatattgg ccggaacgcc
      841 gttagcggta taacccgccc gatgcgtttt atattcggtc agctggcgtt gttggttttg
      901 tttcagattc ggtaaggcca aatcttcaaa gagcagatca agatactcat ctttcgaaat
      961 ctgaaagacg aattcatcct gaccttcacc atcctggctg gcctggccct gaccactgcc
     1021 ggaacctccg ccgccaccct ggggacgttc aattcggtcg ttctggacga aatggtcatt
     1081 gcccggatgt acgcggtggc gcaggccgcc acgcccctga tgaaacatcg gttcgctaat
     1141 atcttccgtg ggaatggata cggattcgcc gctgtcgacg tcagtcaccg aacgcttatt
     1201 aatggcctcg gagatcgact gtttaatttg cgctttataa cggcgtaaaa aacgctggcg
     1261 attcaccatg cttttgtttt tgccgttcag acgccgatca ataaaccagg tcatatgccc
     1321 cccgtactac atttgccaac tgtatgctac gcctgtgttg ccatttctct ggcccacggt
     1381 tcgctgtcgt tgtaggcccg gtagcatgtg taccgggcca gggttgttaa gacgatttac
     1441 gtacgcgcaa ataccattcg cacagtaaac gcacctgttt acgggtgtag cctttttcca
     1501 tcatacggtc gacaaagtcg tcgtgtttct tctgctcgtc ggttgacgtt ttggcgttaa
     1561 acgagataac cggcaacagc tcctcggtat tggagaacat tttcttctcg ataaccgtgc
     1621 gcagtttttc atagctggtc cagttcggat tgcgtccgct gttattggct ctggcgcgca
     1681 gtacgaagtt aacaatctcg ttgcggaaat cttttggatt actgatcccc gccggtttct
     1741 cgattttctc cagctcggcg ttaagagact cgcggtcaaa cagctgcccg gtatccggat
     1801 cgcgatactc ctgatcctga atccagaaat ccgcgtaggt aacataacgg tcgaaaatgt
     1861 tttgcccata ttcggaatag gattcaaggt aggccgtctg gatctctttg ccaataaact
     1921 cggcatattt cgggatcaga taacctttca ggaactccag atagcgttct gcctgctctt
     1981 gtgggaactg ctcgcgctcg atctgctgtt ccaggacgta gaacagatgg accgggtttg
     2041 ccgccacttc tacatgatcg aagttgaaca cgcgggagag gatcttaaac gcaaaacgcg
     2101 tcgacagacc gttcatccct tcatcgacac cggcgtagtc acgatattcc tgatacgact
     2161 tggctttggg atcggtgtct ttcagacttt cgccatcata aacccgcatc tttgaataaa
     2221 tgctggagtt ttctggctct ttcaggcgcg aaagaatgga aaaacgtgac agtgtttcga
     2281 gcgtgccagg ggcgcatggg gcgtgagtca attcactgtg attaagcaat ttctcgtaga
     2341 tttttatctc ttcggaaatg cgcaagcaat acggcacctt cacgatgtaa acacgatcga
     2401 ggaaggcttc gttgtttttg ttattacgga aagtgaccca ttcggactcg ttcgagtggg
     2461 caagaataat cccgttgaac ggcagggcgg agataccttc cgtcccgttg tagttacctt
     2521 cctgagtggc ggttaacagc ggatgcagca ctttaatcgg tgctttaaac atctcaacga
     2581 attccatgat cccctggttg gcgcggcaca gtgcgccgga atagccatag gcgtctgggt
     2641 cattctgcgc gtagtgttcg agtttacgaa tatcgacttt ccctaccagc gcggagatgt
     2701 cttggttgtt ctcatcaccg ggctctgttt tggcgatagc aatttgttgc agaatggacg
     2761 gccagacctt cactacccgg aacttagtaa tatcgccacc aaattcatgc agacgttttg
     2821 ccgcccacgg cgacatgatg gtgccgagat agcggcgagg aatgccatac tctttttcca
     2881 gaatctgcgc atcttcctgc ggattgaaaa gacagaacgg atgatcgttg accgggctac
     2941 gctcaccgtt ggcgctcaat acataaatcg gtacgagctg cattaatgat ttcagtcgct
     3001 cagcaagcga tgatttaccc ccacccacag gccccagcag atacaggatt tgtttcttct
     3061 cttccagccc ctgagccgcg tgtttcagat aagagacaat ctgttcaatc gcgtcttcca
     3121 tgccgtaaaa ctcttcaaat gccggataac gtgcaatgac ccggttagaa aagagtcgag
     3181 aaagtctggg ttcctgggct gtatcgacca tgacaggctc accgatagcc atcaatagcc
     3241 gctcagccgc gttggcataa gcactgcgat cttgccgaca agtggtaaga aactcctgca
     3301 gtgtgaactc ttcgtccttg gcagcttcat atcgctggcg atagtgatcg aatatattca
     3361 tggtatgccg tcctttcgtt ttttagcaca ggttaagagc cgttcatatg aatagtagag
     3421 gctcccggaa gaggtaaact gaacgacgtc tcttgctaaa acacagcaac tctcatgcca
     3481 ttttatcgac gatgagaaca acgtgcccgg tgatacacct tcaaaattaa gcgtagatgg
     3541 caaatggaaa acttgcctgc acggctttct taatttcaat ggcatatcaa caactcatcc
     3601 ataaattttg cataattaat gtaaagacca gggttgccag taacgttaaa ttcatttggc
     3661 tgtaagcgcg gtgtcatccg cgtcaggaaa attaaacagt tactttaaaa aatgaaaacg
     3721 taaaaaggtt gggtttcgat gtattgacgg gtaaactttg tcgcccgcta aacatttgtt
     3781 taaggaatga ttaattgtga ccaaactcaa acttctggca cttggagtgc ttatcgcaac
     3841 gtctgcaggc gtagcgcacg ctgaaggtaa attttccctg ggcgcaggcg taggtgtcgt
     3901 tgaacaccca tataaagatt acgataccga tgtttaccca gtaccggtaa tcaactatga
     3961 aggcgataac ttctggttcc gtggcttagg tggtggttac tacctgtgga atgacgcaac
     4021 ggataaactt tcaattaccg cttactggtc gccgctttac ttcaaagcta aagacagtgg
     4081 cgatcaccaa atgcgtcacc tggatgaccg taagagcacc atgatggctg gtctgtctta
     4141 tgctcacttt acccagtacg gttacctgcg taccaccctg gctggcgata ccctggataa
     4201 cagcaacggc atcgtctggg atatggcctg gttgtatcgt tacaccaacg gtggcctgac
     4261 cgtgactccg ggtattggtg tgcagtggaa cagcgaaaac cagaacgaat actattatgg
     4321 cgtatcgcgc aaagagtccg ctcgcagcgg tctgcgtggc tataacccga acgacagctg
     4381 gagcccttac ctggagctga gcgccagcta caacttcctc ggcgactgga gtgtttacgg
     4441 taccgcgcgc tacacccgtc tgtctgatga agttactgac agcccgatgg tggataaatc
     4501 ctggactggc ctgatttcta ccgggatcac ctacaaattc tgataatgca tctttccagg
     4561 gcattttgtg cataaaaaca gggcgtcacg ccctgttttg cattacggtg gtgcaatacc
     4621 tgggaggaaa caatgcaaca aaaaatgatt caatttagtg gcgatgtctc actgccagcc
     4681 atagggcagg gaacatggta tatgggcgaa gatgccagta agtgtaaaac agaagttgct
     4741 gcactacgcg cgggcattga gctcggttta accctcattg ataccgccga aatgtatgcc
     4801 gatggtggtg ccgaaaaggt ggttggggaa gcattaaccg gtctgcgtga taacgtcttt
     4861 ctcgtctcta aagtctatcc gtggaatgct ggcgggcaaa aagcgataaa tgcatgcgaa
     4921 gccagtttac gccgtctcaa tactgattat ctcgatctct acttattaca ctggtctggc
     4981 agtttcgctt ttgaagagac tgtcgcagcg atggaaaaat tgatcgccca aggaaaaatc
     5041 cgccgctggg gcgtttcgaa ccttgattat gctgatatgc aggaactctg gcagctgacg
     5101 gggggaaatc agtgtgccac taatcaggtg ctttaccatc tcggttcacg agggattgag
     5161 tacgatctac tcccctggtg ccagcaacag cagatgccgg tgatggctta cagtccgtta
     5221 gcccaggccg ggcggttgcg caatggactg ttaaaaaacg cggtagtcaa cgaaattgca
     5281 catgctcaca atatcagcgc ggcacaagta ttgttggcgt gggtaatcag ccatcagggt
     5341 gtgatggcga ttccaaaagc ggccacgatt gcccatgtcc aacagaatgc ggctgtgctt
     5401 gaggtcgaac tttcgtcagc ggaattagcc atgctggaca aagcatatcc ggcaccaaaa
     5461 ggaaaaactg cgctggatat ggtgtgatgt tgatacgtgc cggatgcatc gcacccggca
     5521 cacaacatta acgtaaatta acgtttcgca acgcgaatgg attgcgccag atgtgcaggt
     5581 ttttctttgg tcactttttg cgtttctgaa gcgtaagccg tttctacaca aacaaaggtc
     5641 ttgtagccat cgtccggcat atcgcccatg ctaactgaaa gcgccggtcc cgggttccag
     5701 cctacaacgt tcagatgatg ctggtggcct acggcgataa tacgattcag cgcttcatca
     5761 ttaatcacgc tgcaatcttg cggattcaga tatacgcgat cggtacggtc agggaaggtc
     5821 tgaataccgt cggtcagtac atcttctttc gcgtcattta ctttatcaat gaagcgatcg
     5881 cccagcccac tgacgcttac cttagcgata tcacccacgt taaagtaggt atgcagggca
     5941 gaggtggttt caaattcgcc gtgtgcttca agatcgattt cacaggtttt acccacgcgg
     6001 aaatgcgcta acagcgtaaa gtcgtgcggc cagaattttt tcgtctcttc gctttgtgtc
     6061 aattcaaaag tcagcgctac gccatcagca tcttcacgat gtgatttcag cgtccacggc
     6121 aggttgcggg caaaaccgtg cgcaggcagg ccttgttgtg ccgccggacc aaaccacggc
     6181 cagcaaaccg gtacgccacc gcgaatagcg acgccatttt tgaacggtgt gttgttgctc
     6241 aaccacagaa cttcttcttc acccgcaggt ttccacgaga gaaggtgtgc gccctgtaat
     6301 gcaaaagagg cttttacctg gggatgatcg accacaatga ggtccagttc atccagttta
     6361 cgacgggaga ggacagggga gatttgttcg atgaccggaa gggcaaaaat tttcttaatc
     6421 atgacgcagt cctttaactt cattttatca ggtaaaaaaa agagcgaccg aagtcgctct
     6481 ttttagatca cagtgtcatc tcaacttatt tagagatgtg agcgatcagg tccagaactt
     6541 tgttggagta accggtttcg ttgtcgtacc aggataccag tttcacgaag ttgtcgttca
     6601 gagcgatacc agctttagca tcgaacacgg aagtgcaaac ttcgccgttg aaatcggtag
     6661 atactacgtc atcttcggtg tagcccagaa cgcctttcat ttcgccttca gcagcagctt
     6721 taacggcagc tttgatctgc tcgtaagttg cagctttttc cagacgaacg gtcaggtcaa
     6781 ccacggatac gttcggggtc ggaacgcgga acgccatacc agtcagtttg ccattcagtt
     6841 ctggcagtac tttacctaca gctttagcag caccggtaga ggacgggatg atgttctggg
     6901 aagcgccgcg gccgccgcgc cagtctttgt gagacgggcc atcaacggtt ttctgagtag
     6961 cggtagtagc gtgaacggtg gtcatcagac cttcgatgat gccgaagtta tcgttgataa
     7021 ctttagccag cggagccagg cagttggtgg tgcaggaagc gttggaaacg atgtcctggc
     7081 cagcatattt gtcgaagtta gcgcctttaa cgaacatcgg agtgttgtct ttagacggac
     7141 cagtcataac cactttcttc gcaccagcgg tgatgtgttt acgagcagtt tcgtcagtca
     7201 ggaacagacc agttgcttca gcgacaacgt caacaccaac ttcgtcccat ttcaggttag
     7261 ccggatcacg ttcagcggta acacggattt ttttaccgtt aacgatcaga tgaccgtctt
     7321 tcacttcaac ggtaccgtcg aaacggccgt gagtggagtc atatttcagc atgtatgcca
     7381 tgtaatcagc gtctaacagg tcgttgattg caacgatctc gatgtcagaa cgtttctgag
     7441 cagcacggaa aacaatgcga ccgatacggc caaaaccgtt gatacctact ttgatagtca
     7501 tatattccac cagctatttg ttagtgaata aaaggttgcc tgtaaaatta caaaaacctt
     7561 acgcagcgtc aagcggaatc gtgtcaatca ttgcgacaaa tcaatcctgt gcctaagcat
     7621 tacgcgactg actcgcctca ctcttccttt gggcttgaga ccacatgggg tcggcgcccc
     7681 gaattttaaa gggcaattac gataaaaatg tgattttcat cacgatttag gtgcagccat
     7741 ttcgtcgaga tcaagtttag gacaaaattt tgccagttgg tgttaatgtt ttgttagaat
     7801 cagtcacgta atgtgagcac gattaaagtg agatgtgagc aaatggctaa taaaccttcg
     7861 gcagaagaac tgaaaaaaaa tttgtccgag atgcagtttt acgtgacgca gaatcatggg
     7921 acagagccgc catttacggg tcgtttactg cataacaagc gtgacggcgt atatcactgt
     7981 ttgatctgcg atgccccgct gtttcattcc caaaccaagt atgattccgg ctgtggctgg
     8041 cccagtttct acgaaccggt gagtgaagaa tccattcgtt atatcaaaga cttgtcacat
     8101 ggaatgcagc gcatagaaat tcgttgcggt aactgtgatg cccatctggg gcatgtcttc
     8161 cccgacgggc cgcagccaac gggcgaacgt tattgtgtta actctgcttc tttacgcttc
     8221 actgatggcg aaaacggcga agaaatcaat ggttgaaaaa acgattcagc aacttattcc
     8281 acaggagcgg aaaaattatg aatcttgatg acattatcaa cagcatgacg cctgaggtat
     8341 accagcgttt gtcgaccgcc gttgaactgg ggaaatggcc tgatggcgtt gcgttgaccg
     8401 aggaacaaaa agagaactgc ctgcaactgg tgatgctgtg gcaagcccgc aataataccg
     8461 aagcacagca tatgacaatt gacacaaatg gtcagatggt catgaagagc aaacaacagt
     8521 taaaagaaga ttttggtatc tcagcaaagc ctattgccat gtttaagtaa gtggtttgtc
     8581 ccctttttgc cgcggttata aaggggatag ccaacaataa ccccacgact catctcaatt
     8641 atctcgtcac gaccaggcaa agccccagcc cggtctcttg ccgtcgcgtt cacgcctgaa
     8701 tgacttctcc tgaacttaag tctcagacct atttggccgg taatccctct caaatttgct
     8761 ccttttgcgt tcagccttcc atcaacctgt aacagaacca accattttct gcgtaattgt
     8821 gatctgagtt gtaaaacgca gatcattatc tgtgttttac agatttaatg ttgacctcaa
     8881 aaatcatgtg ggtaagtatg atgaccatct aagcaaaaca aaacacagag gatgacgcaa
     8941 tgaaagcact ggctcggttt ggcaaagcct ttggcggcta caagatgatt gatgtcccac
     9001 aacccatgtg tggcccggaa gatgtagtga ttgaaattaa agccgcggca atctgcggcg
     9061 cagacatgaa gcactacaat gtcgatagcg gttctgatga gtttaactct atccgcggcc
     9121 atgagttcgc aggttgtatt gcgcaggttg gtgaaaaagt caaagactgg aaagtggggc
     9181 aacgcgtcgt atcggataac agcggtcacg tttgcggcgt ttgtccggcc tgtgaacaag
     9241 gtgattttct gtgttgtaca gaaaaagtaa accttggtct ggataataat acctggggcg
     9301 gtggtttttc caaatattgt ctggttcctg gtgaaattct caaaattcat cgtcatgcgt
     9361 tgtgggaaat ccctgatggt gttgattatg aggacgcagc cgtacttgac cctatctgta
     9421 atgcctacaa atccatcgcg cagcaatcga aattccttcc tggtcaggat gtggtcgtca
     9481 tcggcactgg cccactcggg ctgttctccg tacaaatggc gcgaattatg ggggcggtaa
     9541 atatcgtcgt cgttggtctg caagaagatg tggcggtccg cttcccggtt gcaaaagaac
     9601 tgggtgcgac ggcagtagta aatggttcta ccgaagatgt ggtggcgcgc tgccagcaaa
     9661 tttgtggaaa agacaatctg ggactggtga ttgaatgctc cggtgccaat atcgcactga
     9721 aacaagccat cgaaatgctc cgcccgaacg gggaagtggt acgcgttgga atgggcttca
     9781 aacctcttga tttctcgatt aatgacatta ccgcctggaa caaaagcatc attgggcata
     9841 tggcctatga ctccacctca tggcgtaacg ctatcaggct attagccagc ggcgctatca
     9901 aagtcaaacc gatgatcacg catcgtatcg gcctgtcgca atggcgcgaa gggtttgatg
     9961 cgatggtcga taaaaccgca atcaaagtga tcatgactta cgactttgat gaataacaac
    10021 tttcctgaag gaacgttatc gcatggaaca gataacaaaa ccgcattgtg gtgcacggct
    10081 ggatcgctta ccggattgcc gctggcattc gtcaatgttt gccatcgtcg cgtttggctt
    10141 gcttgtctgc tggagtaatg ccgttggtgg cttgatcctc gcgcagctga aagcgttggg
    10201 ctggacagat aattccacca cagccacatt ctcagcaatc acgaccgccg gaatgtttct
    10261 cggtgcgctg gtgggcggca tcattggcga caaaaccggt cgcagaaatg cgttcatcct
    10321 ctatgaggcc attcatattg cctcgatggt tgtcggtgct ttctcaccga atatggattt
    10381 ccttattgcc tgccgttttg tgatgggagt tgggctggga gctttactgg ttacgctgtt
    10441 tgctggtttc accgaatata tgcccggtag aaatcgtgga acgtggtcaa gtcgggtttc
    10501 ctttattggc aactggtcat atccgctctg ttcattgata gcgatgggac tcacgccgct
    10561 gattagtgca gagtggaact ggcgggtaca actgcttatc cctgcaatat tgtcgcttat
    10621 cgctacggcg ctggcctggc gctactttcc tgaatccccg cgctggctgg aatcgcgcgg
    10681 acggtatcag gaagccgaga aagtcatgcg gagtatagaa gaaggcgtca tacaccagac
    10741 gggcaaacct ttgccgcccg tggttattgc tgatgacggt aaaacgccac aagcggtgcc
    10801 gtattcagcc ttactgacag gagtattact gaaacgcgtg atattaggct cttgtgtgct
    10861 gattgccatg aacgttgtgc agtacacact aattaactgg ttgccaacga tattcatgac
    10921 ccaggggatt aatttaaaag actcgattgt tttaaatacc atgagtatgt ttggcgcgcc
    10981 atttggcatt tttattgcca tgctggtgat ggataaaatt ccgcgtaaaa caatgggtgt
    11041 ggggctatta atcctgattg cggtgctcgg atatatctat tcactgcaaa ccagtatgtt
    11101 gctcataacg ttgattggtt tcttcctgat tacttttgtc tatatgtacg tttgctatgc
    11161 ctcggcagtg tatgtccctg agatctggcc gacagaggcc aaactccgtg gctccggtct
    11221 ggcgaatgcg gtagggcgaa tcagtggtat tgccgcacct tatgccgttg cagtgctgct
    11281 cagtagttat ggcgttacgg gagtctttat tcttctgggg gcggtttcaa ttattgtcgc
    11341 aattgctatc gccaccattg gaatcgaaac caaaggtgtc tccgttgaaa gtttaagtat
    11401 tgatgcagta gccaataaat aatacgggtg gattcaaaat gaaaaattca aaagcaatat
    11461 tgcaggtgcc gggcacaatg aaaattattt cagcagaaat accagtgcct aaagaagatg
    11521 aagttttgat taaagtagaa tatgtcggta tttgtggttc agatgtacat ggttttgaat
    11581 caggtccgtt tattccgcct aaagacccaa atcaagaaat tggcctgggt catgaatgcg
    11641 ccgggacggt tgtggctgtg ggaagccgcg tgcgcaaatt taaaccgggg gatcgggtaa
    11701 atatcgaacc tggcgttcct tgcggtcact gtcgttactg tctggaaggc aaatataaca
    11761 tctgcccgga cgttgatttt atggcgacac aacccaacta ccgcggcgca ttaacacact
    11821 atctgtgtca tccggagagc tttacttaca aactgcccga caatatggac acgatggaag
    11881 gggcgctggt ggagcctgcc gcagtcggga tgcatgccgc gatgctggca gatgttaaac
    11941 cgggtaagaa gataattatt ctgggagcag gttgtattgg tttgatgacg ttgcaagcgt
    12001 gcaaatgcct gggagcaact gaaattgccg tcgttgatgt gctggaaaaa cgtctggcaa
    12061 tggcggaaca gcttggtgcg acagtggtta ttaacggcgc aaaagaagac actattgcac
    12121 gctgtcagca atttaccgaa gacatgggcg cagatattgt tttcgaaaca gcgggttctg
    12181 cggtcaccgt taaacaggca ccttatctgg taatgcgcgg cggtaaaatt atgattgttg
    12241 gtactgtacc cggcgattcg gcaatcaatt tcctcaaaat aaatcgcgaa gttactatcc
    12301 agacggtatt ccgctacgcc aatcgttatc cggtcacgat tgaagctatt tcttcagggc
    12361 gattcgatgt gaaatcgatg gtgacgcata tttacgatta tcgggatgta caacaggcat
    12421 ttgaagagtc agttaacaat aaacgcgaca ttattaaagg cgttattaag attagcgatt
//
LOCUS       DAZKFU020000133        12384 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZKFU020000133 DAZKFU020000000
VERSION     DAZKFU020000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.134"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            67..1122
                     /gene="phoE"
                     /locus_tag="ACHOQQ_RS15115"
                     /old_locus_tag="pgaptmp_003023"
     CDS             67..1122
                     /gene="phoE"
                     /locus_tag="ACHOQQ_RS15115"
                     /old_locus_tag="pgaptmp_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU4471242.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1161..1562)
                     /gene="crl"
                     /locus_tag="ACHOQQ_RS15120"
                     /old_locus_tag="pgaptmp_003024"
     CDS             complement(1161..1562)
                     /gene="crl"
                     /locus_tag="ACHOQQ_RS15120"
                     /old_locus_tag="pgaptmp_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4471243.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1620..2864)
                     /gene="frsA"
                     /locus_tag="ACHOQQ_RS15125"
                     /old_locus_tag="pgaptmp_003025"
     CDS             complement(1620..2864)
                     /gene="frsA"
                     /locus_tag="ACHOQQ_RS15125"
                     /old_locus_tag="pgaptmp_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4471244.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2956..3414)
                     /gene="gpt"
                     /locus_tag="ACHOQQ_RS15130"
                     /old_locus_tag="pgaptmp_003026"
     CDS             complement(2956..3414)
                     /gene="gpt"
                     /locus_tag="ACHOQQ_RS15130"
                     /old_locus_tag="pgaptmp_003026"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4471245.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3675..5132
                     /gene="pepD"
                     /locus_tag="ACHOQQ_RS15135"
                     /old_locus_tag="pgaptmp_003027"
     CDS             3675..5132
                     /gene="pepD"
                     /locus_tag="ACHOQQ_RS15135"
                     /old_locus_tag="pgaptmp_003027"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4471246.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5189..5497)
                     /locus_tag="ACHOQQ_RS15140"
                     /old_locus_tag="pgaptmp_003028"
                     /pseudo
     CDS             complement(5189..5497)
                     /locus_tag="ACHOQQ_RS15140"
                     /old_locus_tag="pgaptmp_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(5517..5725)
                     /locus_tag="ACHOQQ_RS15145"
                     /old_locus_tag="pgaptmp_003029"
                     /pseudo
     CDS             complement(5517..5725)
                     /locus_tag="ACHOQQ_RS15145"
                     /old_locus_tag="pgaptmp_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(5916..6368)
                     /gene="yafP"
                     /locus_tag="ACHOQQ_RS15150"
                     /old_locus_tag="pgaptmp_003030"
     CDS             complement(5916..6368)
                     /gene="yafP"
                     /locus_tag="ACHOQQ_RS15150"
                     /old_locus_tag="pgaptmp_003030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4471247.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            complement(6378..6776)
                     /gene="yafO"
                     /locus_tag="ACHOQQ_RS15155"
                     /old_locus_tag="pgaptmp_003031"
     CDS             complement(6378..6776)
                     /gene="yafO"
                     /locus_tag="ACHOQQ_RS15155"
                     /old_locus_tag="pgaptmp_003031"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HGU4471248.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(6779..7072)
                     /gene="yafN"
                     /locus_tag="ACHOQQ_RS15160"
                     /old_locus_tag="pgaptmp_003032"
     CDS             complement(6779..7072)
                     /gene="yafN"
                     /locus_tag="ACHOQQ_RS15160"
                     /old_locus_tag="pgaptmp_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HGU4471249.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(7124..8179)
                     /gene="dinB"
                     /locus_tag="ACHOQQ_RS15165"
                     /old_locus_tag="pgaptmp_003033"
     CDS             complement(7124..8179)
                     /gene="dinB"
                     /locus_tag="ACHOQQ_RS15165"
                     /old_locus_tag="pgaptmp_003033"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU4471250.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8250..9035)
                     /gene="lafU"
                     /locus_tag="ACHOQQ_RS15170"
                     /old_locus_tag="pgaptmp_003034"
     CDS             complement(8250..9035)
                     /gene="lafU"
                     /locus_tag="ACHOQQ_RS15170"
                     /old_locus_tag="pgaptmp_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGU4471251.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            9007..10719
                     /locus_tag="ACHOQQ_RS15175"
                     /old_locus_tag="pgaptmp_003035"
                     /pseudo
     CDS             9007..10719
                     /locus_tag="ACHOQQ_RS15175"
                     /old_locus_tag="pgaptmp_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(10912..11409)
                     /gene="rayT"
                     /locus_tag="ACHOQQ_RS15180"
                     /old_locus_tag="pgaptmp_003036"
     CDS             complement(10912..11409)
                     /gene="rayT"
                     /locus_tag="ACHOQQ_RS15180"
                     /old_locus_tag="pgaptmp_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HGU4471252.1"
                     /translation="MSEYRRYYLKGGSWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKR
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHT
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGNITDFSAG
                     ERIIS"
     gene            complement(11585..12304)
                     /gene="yafL"
                     /locus_tag="ACHOQQ_RS15185"
                     /old_locus_tag="pgaptmp_003037"
     CDS             complement(11585..12304)
                     /gene="yafL"
                     /locus_tag="ACHOQQ_RS15185"
                     /old_locus_tag="pgaptmp_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4471253.1"
                     /translation="MSGLLLICLPFSSFASATTSHISFSYAARQRMQNRARLLKQYQT
                     HLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSS
                     DHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILEAKLPRTANEM
                     YHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLA
                     EPFWQDHFLGARRILTEETIL"
BASE COUNT         3061 a         3176 c         3139 g         3008 t
ORIGIN      
        1 actttcctgt tttcaacggg gctctcccgc agaatattcg cgcgttaatt aaaatcagga
       61 atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc atctgcatcc
      121 gtacaggccg cagaaatata taacaaagac ggtaataaac tggatgtcta tggcaaagtt
      181 aaagccatgc attatatgag tgataacgac agtaaagatg gcgaccagag ttatatccgt
      241 tttggtttta aaggcgaaac acaaattaac gatcaactga ctggttatgg tcgttgggaa
      301 gcagagtttg ccggtaataa agcagagagt gatactgcac agcaaaaaac gcgtctcgct
      361 tttgccggat tgaaatataa agatttgggt tctttcgatt atggtcgtaa cctgggcgcg
      421 ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga ctcctcggcg
      481 cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg gaacaccgac
      541 ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa aaacgaaaac
      601 cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata tgactttggc
      661 ggcagcgatt tcgccattag tggggcctat accaactcag atcgcaccaa cgagcagaac
      721 ctgcaaagcc gtggcacagg caagcgtgca gaagcgtggg caacaggtct gaaatacgat
      781 gccaataata tttatctggc aactttttat tctgaaacac gcaaaatgac gccaataact
      841 ggcggctttg ccaataagac acagaacttt gaagcggtcg ctcaatacca gtttgatttt
      901 ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga aggtatcggt
      961 gatgaagatc tggtcaatta tatcgatgtc ggggctacat attatttcaa caaaaatatg
     1021 tcagcgtttg ttgattataa aatcaaccaa ctggatagcg ataacaaatt gaatattaat
     1081 aatgatgata ttgtcgcggt tggcatgacc tatcagtttt aatgattatt gccggatgtg
     1141 atgcatccgg cacatttcac tcacgccgtt aacttcaccg gctcgtcacg aaaatcatcc
     1201 gccggttcca gcttcagatt cagcgtcgtc agcagttcac gcagtttctc gtgaaactca
     1261 cgcagggtgt gctccagtcg ttcaaccact tcagtgtctt ttaccggaac gctcttccag
     1321 tcgcctgctt tatcgaacag accaaactgg taactgtagg taaaacggga ttcctgcgct
     1381 tcaagctcca tccaccagcc ccagaactca cgcacttccg gtgccggttt cacgttgacg
     1441 catacagcca gacaatcgaa aaagaatcga ttatcttcgc acttaccttc acgaatatac
     1501 gggcctagtg cggtaaattt tttgatcaat ctgctcttcg ggtgtccact cggtaacgtc
     1561 attgctatct cctgttgtga tgcaactgtt ttaccaaatt ggcaaaattt agcaaatttt
     1621 taacacaagc gtttttcgat ccagtcggtg atttcctgaa gacctttgtc aaaattccga
     1681 tacaccgggt taaatgggat ctctaataat ttaccgtcag cagatgatga ggtgattaag
     1741 cgtgagtcct cttccgggct gaacggatcg ttcttccagt agcctgataa cattggcgtt
     1801 gggcagcgac gtcccagcaa tccttgcact tttaatgaat agcgattcag ctccacgcgc
     1861 aacgcttcat cggaagcatc atgcatcccc aaacgactcg ccagaacgtc aagatacatt
     1921 tccggcacct gttgctggca cttaaaatca ctcaacaggg tatgaactac cggaccaaga
     1981 caggcaaccg ctttcagacg cggcgattca agatatgcca gacgcacggc aacgttagcg
     2041 ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac gttaggcagc
     2101 gcctttaaga cgtgctgatg caacaggctg gagtcctggg tgagcttcca ttttgatgaa
     2161 aagcccaccg acggcatatc aatagtcagc atcgcaatgc cgcgcggcgc aaaataacgt
     2221 tcatacaggc tgtaatagtc cgtctgcatc gcatccagac caccacacat taatactgtc
     2281 gggaacgggc catcgccttt cggcatatgc aaaaagccgg tgatgggcgc accgccgggt
     2341 acggtaaact ccatctgccg catcgtgccc ggcagacgct gagcggcctc ttcataggcg
     2401 cggtttgaca aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg aggataggcg
     2461 gcaatgttgt acaacgtagc cgcatgcagc cagtgacgac cactgagttg cggatcttct
     2521 tccgcacagg ctttttgttg ccacaccatc gcctgggtgg cccactcata aatccagttg
     2581 ccgccacggt agccaatcac cgtatcgtat aaatcatcgt cggtacgttc ggcatcgctc
     2641 atcacaatac gtgcctggac gtcgaggatt tcgcgtgggt caatgccgcg ccagatccac
     2701 atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag ggccgactgc
     2761 acaggcggtt gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc aggatgttta
     2821 aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc agcctccaga
     2881 atacttactg gaaactattg taacccgcct gaagttaaaa agaacaacgc ccggcagtgc
     2941 caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc ccatatccca
     3001 cggctgttcg atccaggtat cttgcgggat atcaacaaca taatcatcaa ccagcggacg
     3061 accagccggt tttgcaaaga tggtgacaaa gtgcgctttt ggatacattt cacgaatcgc
     3121 aaccgcagta ccaccggtat ccaccaggtc atcaataacg atgaagcctt cgccatcgcc
     3181 ttctgcgcgt ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc tggaaataca
     3241 aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg gtaccagacc
     3301 gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag
     3361 tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc tcatgtgaag
     3421 tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag
     3481 attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt ctttttacgc
     3541 actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc aagcctgtct
     3601 tgtgttgaca acattttctg ctaaccctgt gacctgcaat actgttttgc gggtgatcga
     3661 caaggagact taacgtgtct gaactgtctc aattatctcc acagccgctg tgggatattt
     3721 ttgccaaaat ctgttctatt cctcacccgt cctatcatga agagcaactc gctgaataca
     3781 ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta ggtaatatct
     3841 tgattcgtaa acctgctaca gcaggtatgg aaaatcgtaa accggtcgtc ttgcaggccc
     3901 acctcgatat ggtgccgcag aaaaataacg acaccgtgca tgacttcacg aaagatccta
     3961 tccagcctta tattgatggc gaatgggtta aagcgcgcgg caccacgctg ggtgcggata
     4021 acggcattgg tatggcctct gcgctggcgg ttctggctga cgaaaacgtg gttcacggcc
     4081 cgctggaagt gctgctgacc atgaccgaag aagccggtat ggacggtgcg ttcggcttac
     4141 agagcaactg gttgcaggct gatattctga ttaacaccga ctccgaagaa gaaggtgaaa
     4201 tctacatggg ttgcgcgggg ggtatcgact tcacctccaa cctgcattta gatcgtgaag
     4261 aggttccagc tggttttgaa accttcaagt taaccttaaa aggtctgaaa ggcggtcact
     4321 ccggcggtga aatccacgtt ggcctcggta atgccaacaa actgctggtg cgcttcctgg
     4381 cgggtcatgc agaagaactg gatctgcgtc ttattgattt caacggcggc acactgcgta
     4441 acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc ggctgataaa gtcgacgccc
     4501 tgaaatctct ggtgaatacc tatcaggaga tcctgaaaaa cgagctggca gagaaagaga
     4561 aaaatctggc cttgttgctg gactctgttg cgaacgataa agctgccctg attgcgaaat
     4621 ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa cggtgtgatc cgcaattccg
     4681 acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg tgtggtgacc atgactgaca
     4741 ataatgtaga aattcactgt ctgatccgtt ctctgatcga cagcggtaaa gactacgtgg
     4801 tgagcatgct ggattcgctg ggtaaactgg ctggcgcgaa aaccgaagcg aaaggtgcat
     4861 atcctggctg gcagccggac gcaaactctc cggtgatgca tctggtacgt gaaacctatc
     4921 agcgtctgtt caacaagacg ccgaacatcc agattatcca cgcgggcctg gaatgtggtc
     4981 tgttcaaaaa accgtatccg gaaatggaca tggtttctat cgggccaaca atcaccggtc
     5041 cacactctcc ggatgagcaa gttcacatcg aaagcgtagg tcattactgg acactgctga
     5101 ctgaactgct gaaagaaatt ccggcgaagt aattatttga tttgctgccg gatggcgttt
     5161 aatcgccttc cggcagtttc atccttcatt atccttcgat aaaagccatc cctgtaaacg
     5221 ttcgtcgcgg gttgccacgt tcaatctggt gatggaacat tcgccgctgt gatttcagcg
     5281 ccgcgctatt ttcctgttgc tgctgctcca gcctccaggc aatcagcaat cgcgccagcc
     5341 gtttgttagc atgctggctg cgctctgact gaaccttcac gctaataccg gatgccagat
     5401 gcgtggcgcg taccgccaag tcggttttat tgacattttg accgcccggc cccgacgaac
     5461 gcagcgtgtc aggataacgc ccggcttcgg tttccagcac cgttaccgcg acgtcttgcc
     5521 gggtaaaaac gaccaacaga gaagaacgct gcgccaatcg ggtagccgcg tccggggtgt
     5581 gtaaatctgg catccccact acgcgctgca tgttgggtaa atttgccgtg gtgtgtaatt
     5641 gttggatcgc taaactctcg atccacaggt catcagatgc gatggtaaat accgcatcgg
     5701 ataagggacg aatatatttg cccatgtacc attccattta tgaataaaaa tcaggaaatg
     5761 gcagacgaat ccagggcaag aaaaaaagaa ttaatcctgg aaaggtctgc aaagagtcat
     5821 tgataaagga tttgtcatga tttacctcct tgcagtttgt ggaatcagaa aacgcgcaca
     5881 taccaggcgg gcgttatttt cattgcaagc tggatttaat gttgcggttt atatcgcata
     5941 taaaaattag taaaccacgc tccccggcat tcaacgcgct gctgcttaac tgtctgaaaa
     6001 ccataacgtt caaaaaaggg ttttgcggtt atgctggcgt ccaccgtaag ttcggattca
     6061 gacttaatcc aaggttttaa caaagcactg gcaaccccac ggcgggtgta ttcagggtca
     6121 acaaataaca tatcgatata atgttcaatg cgggaaataa aaccaaccgg ttgtgcatta
     6181 atgactgcaa cccgcacttg tgatttcgcg agtttctcct tccagcgaga ttcgtcaatc
     6241 tgcgcccagg cagcaatttg ttgtggtgaa taatgctgac tggctgtcat cgtaaccgct
     6301 ctaatgaaaa tagcgcatag ttgctgaaaa tcgccaggct gatagtttct tatttgtatg
     6361 ttattcataa tataaattca aaaacgcatg cgaaacgctt ctgccatttt cccaatttta
     6421 tgcatttggt tgttatctcg agccagttta tgaggttcag gtttcagaat ggcaatgagc
     6481 aaccatgctt gctcatcaaa cgcaccctga caatatacca aatgcgcttc gtcattcgtt
     6541 ctgctgaatt ggcgcaactg tggcggaaat ggattattca catttgccag atgaatatga
     6601 gcaactcgct caaatttgat taatggccag gtaaacgagt cgtcgtagag tgcatcgcga
     6661 ccaaatatat ctggcaaaac accgtcacgc ttataggaaa taaaatccgc cgttaacgca
     6721 tcaagttcct ctgctgtaag ttgcaggcga ataagttttg ttttgaatac ccgcatcctt
     6781 attccttaaa gtcattgaaa tcatcatccg tcatatcatt aagatattgt tcagcgcggc
     6841 gtgtaatttc ctctaatctt gcagcacttg gacggaagcg cgcctttatg ggctttttct
     6901 cctcttgttc agcaagcatg tccattaacg cttcgaatgc gctggcactt aagagatatc
     6961 ctgcggggcg attattagaa agaaccgcaa ccggttgatc aataaagtat ttagctgggt
     7021 ttttacgtaa ctcagtgata ttgaccgatt tttcagcgag aattcgatgc atacagtgat
     7081 cccctcataa taatgcacac cagaatagac ataatagtat acatcataat cccagcacca
     7141 gttgtctttc catttgaggg tcaagcaacg tcacatgcag ccccaccagt cgcacaccgc
     7201 gcccaccgcg gcgttcatcc caggttttac gcgcggtggc gattagatca gctttattca
     7261 gccgcggcca gacgtgctcc tgggtggttt gctgaaaatc gtcgaacttt aatttcaccc
     7321 cctggcgagc aatcagtaaa tcaggtttta cctttgccag acggcgttca agttccggat
     7381 acagccgctc gataatcgct tcacattcag accagtgatg aatatcttcc gccatcgtgc
     7441 gttccacgcc gacggatttt cgcaaccgtt cgctgttaac atcgcgttcg tcaatcccct
     7501 gactacgctc ccacaaaatg cggccaaatt tgccaaagcg tttaagcagc atcaccagat
     7561 cacacttttg cacatcaccg caggtccgca gccccatcgc ttccagtttt gccgccgaga
     7621 ctttgccgac gccggggatt tttgccagcg gtaaggtttg cagaaatgcc ggaacttctg
     7681 ccggagtaat cacaaactgg ccgttgggtt tattcatgtc ggaggcgatt ttggcgagaa
     7741 actttaccgg cgcaacgccc gcagacgccg tcagttgcag ctcgttgaaa atcgtctggc
     7801 ggatttcctg ggcaatgagg gtcgcagaac cgtggcaatg gacgctgtcg gtgacatcga
     7861 gataagcctc atccagtgac aacggctcaa tgcgagaggt gtagcgcgag aagatttcac
     7921 ggatatgatt tgaggcttct ttgtaggcgt caaagcgccc cggaagcaag gtgagatgtg
     7981 ggcataattt gagcgccatc cctgtcggca tagcgctgcg tacgccaaat ttacgcgcgg
     8041 gataattggc ggtgctgatc accccccgac gttcgcggct gccgccaata gcaataggga
     8101 tatcgcgcag ggcgggattg tcgcgcatct ccactgcggc gaaaaagcag tccatatcca
     8161 catgaatgat tttacgcatt actcacctct caacactggt aaagtataca gtgatttcag
     8221 ggtttgagaa atgcgtaaag attcagcatt taacgcgttc gctgcgaaag cacctgctgc
     8281 ttatccagct tttgcaccag cggctgcacc actttatccc catgctgacc aaagtattga
     8341 tacagcgtat cggacgcact tttggtcagc accataatct caatacgccg attgcccgcg
     8401 ctttgcggat ttttggcatc cagcagcatc tggtccgcca ttgcgcttac ctgcatcact
     8461 ttatcttccg gcattccggc ctcttccagc acccgacgag ctgaaagcgc gcgatcgccc
     8521 gaaaggttcc agttgttgta gatattgttt ttgtaggcca tcgcatcggt atgcccggta
     8581 ataataattt tgttatcgag cgagtcgaac actggcgcga gctccaccag caaagtttta
     8641 aagaacggca taatctgcgc gctgccgcgt tcgaacatat tgcgattctg gtcgtcttta
     8701 atcagcacgc gtaatccctg cgggacaatc tccatctcca gattcgcttc catatgcgca
     8761 tcacgggcaa tggtgttgat actggttgcc aactcgccaa gctcggtggc ggatttcttc
     8821 ttcagcagcg ccgttttctc attcacatca cgcgcttttt tctccgtctc ttccggcacc
     8881 gccacggtgg caggttttgg cggatgagac gggctgattt tatttaacgg cgacagccca
     8941 ccaccgttaa aaatcgactg cccgtgcagc gcggcgataa tgctttcacg ctcggattta
     9001 ctgacgctgt tgacaatcaa ctttatcgtc gtcaccaaag gggccgagcg tatttccgag
     9061 gtttctgccc gctttaccct ggacgcgatg cccggcaaac agatggcgat tgacgccgac
     9121 cttaacgccg ggttgatcaa ccaggcgcag gcgcaaaccc ggcgtaaaga tgtcgccagc
     9181 gaggccgatt tctacggcgc gatggacggg gcatcaaagt ttgtgcgcgg ggacgccatc
     9241 gccgggatga tgattctggc gatcaacctg atcggcggtg tctgtatcgg gatcttcaaa
     9301 tacaacctga gcgccgatgc tgccttccag caatatgtgc tgatgaccat cggcgacggg
     9361 ctggtggcgc agatcccttc cctgctgctc tccaccgcgg cggcgattat cgtcacccgc
     9421 gtcagcgaca acggcgatat cgcccatgac gtgcgccacc agctgctggc aagcccgtcg
     9481 gtgctctaca ccgccaccgg gattatgttt gtgctggcgg tggtgccggg aatgccgcat
     9541 ctgccgtttt tgctgttcag cgccctgctt ggctttaccg gctggcggat gagcaaacgc
     9601 ccgcaggcag cggaggcgga ggagaaaagt ctcgaaacgc tgacccgcac catcaccgaa
     9661 accagcgagc aacaggtcag ttgggaaacg ataccgctga tcgagcctat cagtttaagc
     9721 ctcggttaca agctggtggc gctggtggac aaagcccagg gcaacccgct cacccagcgt
     9781 attcgcggcg tgcgacaggt gatatccgac ggcaacggcg tgctgctgcc ggagatccgc
     9841 attcgggaaa acttccgcct taagcccagc cagtacgcta ttttcatcaa cggcattaag
     9901 gctgatgaag cggatattcc ggcggataaa ctgatggccc tgccctccag cgaaacctac
     9961 ggcgagattg acggcgtgct ggggaacgac ccggcgtacg ggatgccggt gacctggatc
    10021 cagcctgcgc agaaagcgaa ggcgctgaat atggggtatc aggtgatcga cagcgccagc
    10081 gtgattgcca cccatgtgaa taagattgta cgcagctata ttcctgattt gtttaactat
    10141 gacgacatca cacagttgca taaccgtttg tcgtcgatgg caccgcgcct ggcagaagac
    10201 ttaagcgcgg cgctgaatta cagccagttg ctgaaagtgt accgcacact actgaccgaa
    10261 ggcgtttccc tgcgcgatat cgtcaccatc gccaccgtgc tggtcgccag tagcgcggtg
    10321 actaaagatc atattctgct ggcggccgat gtacgcctgg cgctgcggcg cagcattacc
    10381 catccgttcg ttcgcaagca ggagctgacg gtgtatacgc tgaataatga gctggaaaat
    10441 ctgctcacca atgtggtgaa tcaggcgcaa cagggcggca aagtgatgct cgacagcgtg
    10501 ccggttgacc cgaatatgct caaccagttc cagagtacga tgccgcaggt aaaagagcag
    10561 atgaaagcgg cggggaaaga cccggtgctg ctggtgccgc cgcagttgcg tcctttgctg
    10621 gcgcgctatg cgagattgtt tgcgccgggg ctgcatgtgc tgtcgtataa cgaagtgccg
    10681 gatgagctgg agttgaagat tatgggggcg ttgatgtagg tatccggcat cggtgcacaa
    10741 ccgccggatg cggcgtgaac gccttatccg gcctaccgat ccggcacagg tttgtaggca
    10801 tgataagacg cgccagcgtc gcatcaggca tccgcgcaca attgccggat gcggcacaag
    10861 tttgtaggca tgataagacg cgctagcgtc gcatcaggca tccgcgcaca attatgaaat
    10921 gatacgctcc ccggcactaa aatccgttat gttacccgcc caatcaatgg gatataaccc
    10981 tcgcgccaca tcgcgatgga acgttgagaa tggccaatca ctcacttgct ttacccagcc
    11041 atgttttact ggatttatat aaatataatc aacatgatgg cgatagtctt tggtattgcg
    11101 gatggtgtgc tcccaaaaac gtggttgcca gatatttttc aatccacaag catgggtgaa
    11161 ttgcttttta atctcccgcc agcgagagga aaaatcatca tcgctttcag gtaatgtcca
    11221 gatacagtgc atatgctccg gcaaaactac ccaggcatta atttcaaaag gtctggctcg
    11281 cttaacttta ataatggcgt tacggagcgt ctggtactgg gtagtcaaaa gttggcttcg
    11341 acgattccgt aaattcaccg tgaaaaacca gcttcccccc ttgaggtaat aacgacgata
    11401 ttcagacata gcgactcctt tttctcaacg gtaagaagaa aaggaaaggt tggcaatgag
    11461 gttgttttat atcgggaaag cggctcgcaa atattcgcgg gctcgtaggc atgataagac
    11521 gcgccagcgt cgcatcaggc atcggtgcac aactgccgga tgcggcgtaa acgccttatc
    11581 cgtcctacaa aatcgtctct tccgtcaaaa tccggcgcgc gcctaaaaaa tgatcctgcc
    11641 agaaaggttc agctaaccga cttacccgaa tggtttcccc ggtgcgcggt gactcaataa
    11701 actgaccgtc gcccagatac acccccatat ggtcggcaat ctcgcggctg tggatatgga
    11761 aaaacagcaa atctccccgg cgcaggtcgt tattcgccac aatcgttgcc cggcggtagt
    11821 gatacatctc attggccgtg cgcgggagct tcgcctcgag gatcttgttg taggcataaa
    11881 aaaccaaccc gctacagtcg aagcctttat cgggccgcgt gccgccccag acgtaaggct
    11941 tgccaagctg ctgctccagg cggtgaatcg ccacttcggt gatgttatgc aggtgatcgc
    12001 tgcttaaaaa gtggttgttt tccgccagcg cctgccagcg tctgtcactc gccttgagcg
    12061 gcgcaggaaa ccattctgga tgctgtttta tctgttcgcg gttgtgctgg cgcagcgccc
    12121 ttctgctttc ggcattgcct tccacaatat agctggcctg ctttttcaga tgggtttggt
    12181 actgttttaa taaacgcgca cggttttgca tccgctggcg ggcggcgtag ctgaaagaga
    12241 tatgtgatgt ggtggcgctg gcaaatgaag aaaacggcaa acaaatcaac agtaatcccg
    12301 acaatacaaa ggatggaatc gacggcaagg acatactcaa taatggctgg tcatgcttta
    12361 atgttgcagt gtatcccgga tcgt
//
LOCUS       DAZKFU020000134        12172 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZKFU020000134 DAZKFU020000000
VERSION     DAZKFU020000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.135"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            246..1634
                     /gene="narU"
                     /locus_tag="ACHOQQ_RS15190"
                     /old_locus_tag="pgaptmp_003038"
     CDS             246..1634
                     /gene="narU"
                     /locus_tag="ACHOQQ_RS15190"
                     /old_locus_tag="pgaptmp_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGU4471254.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASITDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1729..5469
                     /gene="narZ"
                     /locus_tag="ACHOQQ_RS15195"
                     /old_locus_tag="pgaptmp_003039"
     CDS             1729..5469
                     /gene="narZ"
                     /locus_tag="ACHOQQ_RS15195"
                     /old_locus_tag="pgaptmp_003039"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGU4471255.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGELRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     KELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5466..7010
                     /gene="narH"
                     /locus_tag="ACHOQQ_RS15200"
                     /old_locus_tag="pgaptmp_003040"
     CDS             5466..7010
                     /gene="narH"
                     /locus_tag="ACHOQQ_RS15200"
                     /old_locus_tag="pgaptmp_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4471256.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7010..7705
                     /gene="narW"
                     /locus_tag="ACHOQQ_RS15205"
                     /old_locus_tag="pgaptmp_003041"
     CDS             7010..7705
                     /gene="narW"
                     /locus_tag="ACHOQQ_RS15205"
                     /old_locus_tag="pgaptmp_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4471257.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            7702..8382
                     /gene="narI"
                     /locus_tag="ACHOQQ_RS15210"
                     /old_locus_tag="pgaptmp_003042"
     CDS             7702..8382
                     /gene="narI"
                     /locus_tag="ACHOQQ_RS15210"
                     /old_locus_tag="pgaptmp_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4471258.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8462..9355
                     /locus_tag="ACHOQQ_RS15215"
                     /old_locus_tag="pgaptmp_003043"
     CDS             8462..9355
                     /locus_tag="ACHOQQ_RS15215"
                     /old_locus_tag="pgaptmp_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGU4471259.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDDYRISLEQGTPGFEPPLEDETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9451..10296)
                     /gene="nhoA"
                     /locus_tag="ACHOQQ_RS15220"
                     /old_locus_tag="pgaptmp_003044"
     CDS             complement(9451..10296)
                     /gene="nhoA"
                     /locus_tag="ACHOQQ_RS15220"
                     /old_locus_tag="pgaptmp_003044"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HGU4471260.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALRLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVPDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10469..11038
                     /locus_tag="ACHOQQ_RS15225"
                     /old_locus_tag="pgaptmp_003045"
     CDS             10469..11038
                     /locus_tag="ACHOQQ_RS15225"
                     /old_locus_tag="pgaptmp_003045"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGU4471261.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEHNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEQYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11042..11269)
                     /gene="pptA"
                     /locus_tag="ACHOQQ_RS15230"
                     /old_locus_tag="pgaptmp_003046"
     CDS             complement(11042..11269)
                     /gene="pptA"
                     /locus_tag="ACHOQQ_RS15230"
                     /old_locus_tag="pgaptmp_003046"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HGU4471262.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11864..>12172)
                     /locus_tag="ACHOQQ_RS15235"
                     /old_locus_tag="pgaptmp_003047"
     CDS             complement(11864..>12172)
                     /locus_tag="ACHOQQ_RS15235"
                     /old_locus_tag="pgaptmp_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471263.1"
                     /translation="NIALEQVVTWDSDNIFTPNIFEHNGRCFMKKNETWMLLNGDATP
                     NIRTMSNKKIECLEKSEVEWASRVCDSITDKERENLKALICSKPAKSVNTDKRPALGY
                     "
BASE COUNT         2867 a         3159 c         3147 g         2999 t
ORIGIN      
        1 atctgaaggg ttgaatcatg acatgcgtac atagctgaac cccgtttaat ggttagcttg
       61 ttagttacat ttagtaacac ataactggct gcttaatccc cattctgccc ccgcggttac
      121 gctttcttat ctattctttc aatcagcact tcacccaatt catccttaat tctaataact
      181 ggctattgcg ccttacctcc tgttttgtct tttttcatta ccaatgtgtg catgtgagga
      241 acaatatggc actgcaaaat gagaaaaata gtcgttatct tttacgcgac tggaaaccag
      301 aaaatccggc cttctgggaa aataaaggaa aacatattgc tcgaagaaac ctctggatat
      361 cagtcagttg tctacttctt gccttctgtg tctggatgct atttagcgca gttaccgtta
      421 atctcaataa aatcggtttt aattttacta ccgatcaact ctttttatta accgcattac
      481 cctccgtttc tggcgcatta ttgcgtgttc cctactcctt tatggtacct atattcggtg
      541 gacgccgatg gacggttttt agtactgcaa tcctgattat tccttgcgtc tggctcggaa
      601 ttgccgtgca gaatccgaat actccttttg ggatctttat cgttatcgct ttgctatgcg
      661 gttttgcagg tgcgaacttt gcttcgagca tgggcaatat cagtttcttc tttccaaaag
      721 ccaggcaagg gagcgctctt gggattaatg gcggattagg aaacttaggt gtaagtgtga
      781 tgcagctggt tgcaccgctg gtcatttttg tacccgtatt cgcctttctc ggcgtcaatg
      841 gcgtcccgca ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg atttgggtac
      901 cgctactggc gattgccacg atcgccgcct ggtcagggat gaatgatatc gccagttcac
      961 gcgcgtcaat taccgaccag ctccctgtct tacaacgcct gcatctctgg ctgctgagcc
     1021 tgctttacct tgccaccttc ggttcgttta tcggtttttc tgcgggtttt gccatgctgg
     1081 caaaaaccca gttcccggat gtgaatattc tgcgcctggc gttctttggc ccatttatcg
     1141 gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa attcggcggc gtgcgggtga
     1201 cgttgatcaa ctttattttt atggcgattt tcagcgccct gctgttcctt accttaccgg
     1261 gcacaggctc cggtaatttc atcgcatttt acgccgtatt tatggggctg tttctgaccg
     1321 cgggtctggg aagtggttct actttccaga tgatcgccgt catctttcgc cagataacca
     1381 tttatcgggt gaagatgaaa ggcggtagtg atgagcaagc tcagagagaa gctatcaccg
     1441 aaacggcggc ggctctgggc tttatctcag ccattggcgc agtgggcggc ttttttattc
     1501 cgcaggcatt tggcatgtcg ctcaatatga ccggctctcc agtgggcgcg atgaaagtgt
     1561 ttttaatctt ctacatcgtt tgtgtgctac tgacctggct ggtttatggt cggcggaaat
     1621 tcagccaaaa ataagagatc gatgcctgac acgtcttatc aggctacaac cgtgtcgcat
     1681 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1741 ttggatcgct ttcgctactt caaacaaaag ggcgatacct ttgccgatgg tcacggacag
     1801 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     1861 atcgtgcgtt ccacccacgg tgttaactgt acaggctcct gtagctggaa aatctacgtt
     1921 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     1981 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2041 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2101 gccctcaaac aacacagcga tccggtactt gcgtgggcat cgattatgaa cgatccgcaa
     2161 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2221 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2281 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaaca
     2341 cgttatctgt cactattagg tggcacctgt ttaagcttct acgactggta ttgcgacctg
     2401 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg tgccggaatc tgccgactgg
     2461 tataactcca gttacatcat cgcctggggg tctaacgtac cgcagacacg tacgccggac
     2521 gcccatttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2581 tactctgaag tggccaaatt gtgcgaccag tggctggcac caaaacaagg cactgatagc
     2641 gccctggcga tggcaatggg ccatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2701 gactacttta tcaactactg ccgccgctat agcgacatgc cgatgctggt aatgctggaa
     2761 cctcgcgacg acggtagcta cgtacccggg cgcatgatcc gcgcgtctga cctggtagat
     2821 ggactaggcg aaagcaataa tccgcagtgg aaaaccgtgg cggttaatac cgcaggtgaa
     2881 ttggtagtgc cgaatggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     2941 gaatccattg ccgccggtaa ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3001 gccgttgcag gcgtggcttt cccctacttt ggcggcatcg aaaatccgca ttttcgcagc
     3061 gtaaaacaca atcctgtcct ggtgcgtcaa ttacccgtta aaaacctgac attagccgat
     3121 ggcagcacct gtccagtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3181 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccct
     3241 gcctggggcg agcaaattac cggcgttccg cgccagtaca ttgaaaccat cgcccgcgaa
     3301 tttgccgata ccgctcataa aacgcatggg cgctcgatga ttatcctcgg tgctggtgtc
     3361 aaccactggt atcacatgga catgaactac cgcgggatga tcaacatgct gatcttctgc
     3421 ggttgtgtcg gacaaagcgg tggcggctgg gcacactatg tcggtcagga aaaactgcgc
     3481 ccgcaaaccg gctggttgcc gctggccttt gcgctcgact ggaaccgacc accacgccag
     3541 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3601 gcgcaggagt tactttcacc gcttgccgat gccagtaagt acagcggtca tctgattgat
     3661 ttcaacgttc gcgccgaacg catgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3721 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3781 caggcgctga aatcgggcga gttgcgtatg gcctgcgaac aaccagatag cggcagcaat
     3841 catccgcgta atttgtttgt ctggcgttct aacctgcttg gctcctccgg caaaggccac
     3901 gagtatatgc agaagtatct gctggggacc gaaagcggga ttcagggcaa ggaactcggt
     3961 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4021 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4081 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatccg
     4141 tttattcatc cgctatctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4201 atctacaaag gtattgccaa agcattttcg caagtgtgtg tgggtcatct tggcaaagaa
     4261 accgacgtgg tattgcaacc actgctgcac gactctccgg cagagctctc acagccgtgt
     4321 gaagtgctcg actggcgcaa aggcgaatgc gatctgattc caggtaaaac cgcgccgaat
     4381 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4441 ttgatggaca aacttggcaa cggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4501 gatttcctcg gtaaactcaa ttacaccaaa cgtgatggcc cggcacaggg gcgtccgctg
     4561 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccaga aaccaacggt
     4621 catgttgcag ttaaagcgtg gcaggcgctg ggcgagatca ccggacgcga acatacccat
     4681 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4741 aaaattatct ccagccccac atggtctggt ctggaaagcg atcacgtctc ctataatgcg
     4801 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     4861 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     4921 atcgacaccc gtagcgtcag tgagatgcgc cagatcccgc caaacggctt cccggaaaaa
     4981 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5041 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aaccgatgcc
     5101 cgtgaactaa ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5161 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5221 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5281 aactcggtta cccgcgtttg cccgaaacca acgcatatga ttggcggtta cgcgcagctg
     5341 gcctggggct ttaactacta cggcaccgtc ggctcgaacc gcgacgaatt catcatgatc
     5401 cgcaaaatga aaaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5461 aaaaaatgaa aatccgttcg caagtcggca tggtgcttaa tctcgataaa tgtatcggct
     5521 gccatacctg ttcggtgacc tgtaaaaacg tctggaccgg tcgcgaaggt atggagtacg
     5581 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5641 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatacgc ccgcgtctgg
     5701 gcagcaagat gggtgtaata accaaaatct tcgccaaccc ggtggtgccg cagattgatg
     5761 attactacga acctttcaca ttcgactacg aacatttgca tagcgcaccg gaaggcaaac
     5821 atatccctac tgctcgcccg cgttcgctga ttgacggtaa gcggatggac aaagtgatct
     5881 gggggccaaa ctgggaagaa ctgctgggcg gcgagtttga aaaacgtgcc cgcgaccgca
     5941 acttcgaggc catgcaaaag gagatgtacg ggcagtttga aaacaccttc atgatgtacc
     6001 tgccgcgcct gtgcgaacac tgcctcaatc ccagctgcgt ggcgacctgc ccaagcggcg
     6061 ctatctacaa acgcgaagaa gacggcattg tgctgattga tcaggataaa tgccgtggct
     6121 ggcgtttgtg cattagcggt tgtccgtaca aaaaaatcta cttcaactgg aaaagcggca
     6181 agtcagaaaa atgtatcttc tgttacccac gaattgagtc cggtcaaccg accgtgtgct
     6241 cagaaacctg cgtgggtcgc atccgatatc tgggcgtgct gctttacgac gccgaccgca
     6301 ttgaggaagc ggcaagcacc gagcgcgagg ttgacctcta tgaacgtcag tgcgaagtgt
     6361 tcctcgatcc acacgatccc tcagtgatcg aagaagccct gaaacaaggt attccacaaa
     6421 acgtgattga cgctgcccag cgttcgcctg tctacaaaat ggcgatggac tggaaactgg
     6481 cgctaccgtt gcaccctgaa taccgcaccc tgccgatggt ctggtacgtt cctccgctgt
     6541 caccgattca gtcttacgct gatgcgggcg gtttgccgaa aagcgaaggc gtgctccccg
     6601 ccatcgaaag cctgcgtatt ccggtgcaat atctcgccaa tatgttgagt gccggtgata
     6661 ccggtccggt actgcgggcg ctgaaacgga tgatggcgat gcgtcactat atgcgttcac
     6721 aaaccgtgga aggcgttact gatactcgtg ccatcgacga agtaggcctg agcgtcgccc
     6781 aggtcgaaga gatgtatcgc tatctcgcca ttgccaacta tgaagatcgt tttgttatcc
     6841 cgaccagcca tcgggaaatg gcgggcgatg ccttcgcaga acgcaacggc tgcggtttta
     6901 cctttggcga cggttgccac ggttcggaca gtaaattcaa cctgttcaac agtagccgta
     6961 tcgatgccat caacatcacc gaagtgcgcg acaaagcgga gggcgaataa tgcagatcct
     7021 caaagtgatc ggtctgttga tggagtatcc ggacgagctg ttgtgggaat gcaaggagga
     7081 cgcgctggcg ttgatccgcc gcgacgcgcc gatgcttacg gatttcaccc acaacctgct
     7141 taacgcgccg ctgctggata aacaggccga atggtgcgaa gtgtttgacc gcgggcgcac
     7201 tacgtcgctg ctgctgttcg aacatgttca tgccgagtcc cgcgatcgcg gccaggcaat
     7261 ggtggacctg ctggcggagt atgaaaaggt cggcctgcag ctggattgtc gggaactgcc
     7321 cgattatcta ccgctgtatc tggagtattt aagcatactg cctgacgatc aggcgaaaga
     7381 aggattgctc aatgttgcgc cgatcctcgc cctgcttggc ggtcgcttaa aacaacgcga
     7441 ggcaccgtgg tacgcgttgt ttgatgctct gctgcaactg gcgggaagta cactttcaag
     7501 tgacagtgtc acaaaacaag taaacagcga agagcgcgat gacacccgcc aggcgcttga
     7561 tgcagtgtgg gaagaggaac aggtgaagtt tattgaagat aacgccacgg cgtgtgacag
     7621 ctcgccgctt aatcaatatc agcgacgctt tagccaggat gtcgcgccgc agtatgtcga
     7681 catcagtgcg ggaggtgaga aatgattcag tatctgaacg tcttttttta cgatatctac
     7741 ccgtacattt gtgcgacggt gtttttcctc ggcagctggc tgcgctacga ctacgggcag
     7801 tacacctggc gcgcctcctc aagtcagatg ctcgataaac gcgggatggt gatatggtcg
     7861 aatctgttcc atatcggcat tttggggatc ttcttcgggc acctgttcgg catgttaacg
     7921 ccgcactgga tgtacgcgtg gtttttgcca gtggcagcga aacaactaat ggcgatggtg
     7981 ctcggtggta tttgcggtgt gttgacgttg attggcggcg caggactgct gtggcgcagg
     8041 ctgacaaatc agcgcgtgcg tgccacttcc actacgccgg atatcatcat catgagcatt
     8101 ttgctggtcc agtgtctgtt ggggctaagc accataccgt tttcagcaca gtatcctgat
     8161 ggtagcgaaa tgatgaagct ggtgggttgg gcgcaaagca ttgtcacttt ccgtggtgga
     8221 tcgtcagaga tgcttaacgg tgtagcgttc gttttccgcc tgcatctggt gttgggaatg
     8281 accatcttcc tgctcttccc gttcacccga ctggtgcatg tgtggagcgc gccgtttgag
     8341 tacttcaccc gccggtatca gattgtgcgc tcgcggcgat aattttcacc ctggccggat
     8401 agcccttcgc tctccggcca cttgcaggct atgatagttc tccagccaca ggagaacgtt
     8461 aatgaaaccg caggtttacc acgtcgatgc ttttacctct caaccatttc gcggcaattc
     8521 tgccggtgtt gttttccccg ccgataatct tagcgaagcg caaatgcagc ttatcgcccg
     8581 cgaattaggt cattcggaaa ctgcttttct gctgcacagc gacgacagcg atgtgcgcat
     8641 ccgctacttt acgccaacgg ttgaagttcc gatttgtggt cacgcgacag tagctgcgca
     8701 ctatgtacgt gcgaaggtgt taggtttagg aaattgcacg gtctggcaaa catcgctggc
     8761 aggaaaacat cgcgtgacta tcgaaaagca ccacgatgat tatcgtattt cgctggaaca
     8821 aggtacgccg ggctttgagc caccgctgga agatgaaaca cgtgcggcga ttatcaacgc
     8881 gcttcatctc actgaggacg atattctgcc aggcttgcca attcaggtgg caacaacagg
     8941 acactcaaaa gtgatgatcc cgttgaaacc agaagtggat atcgacgccc tttcgcctga
     9001 tcttgctgcg ctgagcgcta tcagcaaaca gattggctgc aatggtttct tcccgttcca
     9061 gatccgccca ggcaaaaacg aaaccgatgg tcgcatgttc tcgcctgcga ttggtattgt
     9121 ggaagatccg gtgactggca atgccaatgg cccgatgggt gcatggttgg tacatcacaa
     9181 cgtattgccc cacgatggca aggtgctgcg tgttaaaggc catcaggggc gcgcattggg
     9241 gcgtgacggc gtgattgaag tgacggtgac aattcgtgat aaccaaccgg aaaaagtcac
     9301 catttctggc gcggcggtga ttttgtttca tgccgaatgg gcaattaaac tctgaaaccg
     9361 ttgcctgatg cgcttcgctt atcaggtcta caaccgagct aatgtatgcc ggataaggcg
     9421 tttacgccgc atccggcaac ctgacataaa ttattttccc gcctccgggt gcgtatcaaa
     9481 cgccgccatc accagcgcta actcatccac ggtaaagcca tgtttcgcat catccacgcc
     9541 cagaccaaac tgttcttgca tcacagcata taatgacgcc acatccggta gatttcgctg
     9601 ctccaccgcg tgcccatttt cataatgggt aaaatgaaaa ttggtcagtg tcagcttgcc
     9661 gccgtccggc aaatggcggc acatcagcaa atgatggcga aaatgcgact gcggccagtg
     9721 cgccgaccag aaattgccca tcacataatc gctttgttgc tgctcgcaga gatcaaaacg
     9781 gtacatcgac tgccaatgct gatgatgatt aaactgcaac acccaatcat caccctcctg
     9841 caacaaccga tactctccgt gtggcgtggt ctgcacgaga tcgggaacta aacgaatcgg
     9901 cgcggttagc gtctgcccac cgaaaccgac atcagcaatc catttttccc cttccagttc
     9961 aaccaacagc aaacgatggg tgcgcggcgg taatgctggc ggatttgata acactacccg
    10021 ccctaacaag ctgcgaacgt taaaccccag ctcgcgtaac acccgctcaa acacgccatt
    10081 ctgctcaaaa cagtaaccgc cacgacgggc tatcaccagt ttctcttcca gcgattgatc
    10141 atcaagctgt atttccctcg gcagcaaaac gtcgaggttt tcaaacggaa tggtgcaatt
    10201 gtgtttcagg cgcaatgcac gaagcgtatc aatattgacc gcagcagctc ccgaccagtt
    10261 aatacgggca aaatagtgat tcagaatggg cgtcatcagg cgtttccctt agtggttttc
    10321 tcaacttata acctggaata gacgccgcac cggctatttt gtgcgatcgc ggacatgcat
    10381 cacgttcgca aatcgccaat cccgttaatt tcgttactta ttgcgttacg gactacgctt
    10441 aaaaaaacct tcttcgcgag gcgataccat gagccgattc atccccatag aattacacca
    10501 tgccagccgt ctgttaaatc atggcccaac tgtcttgatc accagtttcg atgagcaatc
    10561 ccagcggcgt aacattatgg ctgcagcctg gtcaatgccg gtggagtttg aacctccacg
    10621 cgtggcgatt gtggtagata aatcgacatg gaccagggag ttgattgaac ataacggtaa
    10681 atttggcatc gttatcccgg gcgttgcagc aactaactgg acgtgggcag tgggaagtgt
    10741 gtcggggcgt gaagaagata aatttaattg ctatggcatt ccggttgtga gaggcccggt
    10801 atttggtttg cctctggtcg aggaaaaatg tctggcgtgg atggagtgtc gcctgctacc
    10861 tgcgacttct gcgcaagaac aatacgacac actgtttggc gaggtagtat cagcagcggc
    10921 agacgcacgg gtatttgtcg aaggccgctg gcagtttgat gatgataagc tcaatacgtt
    10981 gcatcattta ggtgctggca cgtttgttac tagcggcaag cgtgttacgg cgggttaagc
    11041 attacatgct gtaaccaggt ttctttatca aagcctccat ttggggcgcg atttcggcat
    11101 cccagatagc ttgccatgat tctggttgaa tctgctgtag agaaatgctt atcgagctat
    11161 ctttactgtt cagatggcga ataataacgt cggtaatatc tgcagcaagt gctgcttttt
    11221 gttgttcgtc cagttcacgc ggaaaacatt taatgtcgat gtgcggcatg attgtgctcc
    11281 ttttgaaaga tgtcagtaaa attaacacac attgtgaatg tcgtatgtcc ggtgatgcgt
    11341 ttttacgaca ataatgccta cttgaaattg tagatatttg cactacaccc ttttaaataa
    11401 agcaccaaca aatcgtcaac ttgcagaaca tattcttctg cctacatacc attaagaaaa
    11461 catcaagttc ccgacttgcc aatttattaa cgcccattat agacgctgaa acaagctacg
    11521 gacaaaggag atgccaaaat taccggcaat gcagaaaaac tcaacgaacc atggctcttt
    11581 accacagcag ccggggttgt ttgggaccga cttccgacag cagatcccgg tgggcctgcc
    11641 accatctctt cacaagctta tgcttattac atgcaaaacg tcccgttttg catctttgtg
    11701 cctacggcac acagtgatct ttccctggtc cctgtcccgg attgtagtac cggtgccagt
    11761 tgtagtgcag ttcggattcg tcgtcatact gctagcccgg atggcggagc agttgctcta
    11821 gtcactcaga atattgtaat agtgatagag tgcagtgccg gtttcaatat cctaacgctg
    11881 gtcttttgtc tgtattaact gatttagctg gtttgctaca tattaatgcc ttcagatttt
    11941 ccctttcctt gtctgtgata ctgtcgcaaa ccctactagc ccattctact tcactcttct
    12001 ccaggcattc tattttctta ttgctcattg ttctaatgtt tggtgttgca tctccattta
    12061 ataacatcca tgtttcattt ttcttcataa aacatcttcc attatgttca aagatattag
    12121 gtgtgaatat attatcagaa tcccatgtta cgacttgctc gagggctata tt
//
LOCUS       DAZKFU020000135        12122 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZKFU020000135 DAZKFU020000000
VERSION     DAZKFU020000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.136"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            214..441
                     /gene="traY"
                     /locus_tag="ACHOQQ_RS15240"
                     /old_locus_tag="pgaptmp_003048"
     CDS             214..441
                     /gene="traY"
                     /locus_tag="ACHOQQ_RS15240"
                     /old_locus_tag="pgaptmp_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HGU4471264.1"
                     /translation="MIKGALRAAPSRKVLLTLDEATNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVTEERESTFKEL"
     gene            476..829
                     /gene="traA"
                     /locus_tag="ACHOQQ_RS15245"
                     /old_locus_tag="pgaptmp_003049"
     CDS             476..829
                     /gene="traA"
                     /locus_tag="ACHOQQ_RS15245"
                     /old_locus_tag="pgaptmp_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HGU4471265.1"
                     /translation="MNTSVSVPGAFTPVKKKSSFFSKLNLLRLVRAVIPAAVLMMFFP
                     QMALAAGGQDLMQSGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLAGFA
                     IISVFIAVGMTVVGL"
     gene            844..1155
                     /gene="traL"
                     /locus_tag="ACHOQQ_RS15250"
                     /old_locus_tag="pgaptmp_003050"
     CDS             844..1155
                     /gene="traL"
                     /locus_tag="ACHOQQ_RS15250"
                     /old_locus_tag="pgaptmp_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HGU4471266.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSFWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            1177..1743
                     /gene="traE"
                     /locus_tag="ACHOQQ_RS15255"
                     /old_locus_tag="pgaptmp_003051"
     CDS             1177..1743
                     /gene="traE"
                     /locus_tag="ACHOQQ_RS15255"
                     /old_locus_tag="pgaptmp_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HGU4471267.1"
                     /translation="MEHGARLSTSRVLAIAFIFMSLLIVLSLTVNVIQGVNNYRLQNE
                     QRTAVTPMGFNMPFAVSQNSADASFLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNKMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            1730..2458
                     /gene="traK"
                     /locus_tag="ACHOQQ_RS15260"
                     /old_locus_tag="pgaptmp_003052"
     CDS             1730..2458
                     /gene="traK"
                     /locus_tag="ACHOQQ_RS15260"
                     /old_locus_tag="pgaptmp_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HGU4471268.1"
                     /translation="MRKNKTVIISACLLFSGSVLAGNGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGETAGAWETSTPYESLLVSISQAVRAGQLPEGWYQI
                     PVTKETLQAPAGMSAKAEAVWTGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMF
                     SQPVSQLLVAARMDVYVIRDGEGN"
     gene            2458..3888
                     /gene="traB"
                     /locus_tag="ACHOQQ_RS15265"
                     /old_locus_tag="pgaptmp_003053"
     CDS             2458..3888
                     /gene="traB"
                     /locus_tag="ACHOQQ_RS15265"
                     /old_locus_tag="pgaptmp_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HGU4471269.1"
                     /translation="MASINTIVKRKQFLWLGIVIVGSVSAIGGALYLSDMDMSGNTDA
                     VAGQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRREMDVLNKQRGDD
                     QRRIEKLGQENAALAEQVKALGANPVTVTGEPVPQMPASPPGPEGEPQPGNAPVAFPP
                     QGAGAVPPPTAFYPGNGATPPPQVTYQSVPVPNRIQRKTFSYDAGTKQTPSLPYIPSG
                     SFAKAILIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVS
                     SERAIVRTRNISCLKEGKTIDMPVKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVD
                     GIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVI
                     PIGAGNEVTVVFQDGFQLKTVEEMALEQAQGQVEESNPGSPVPNPPSAESHLNGLNTD
                     QMLKQLGDLNPQQFMAGGQEGGNDGK"
     gene            3878..4462
                     /gene="traP"
                     /locus_tag="ACHOQQ_RS15270"
                     /old_locus_tag="pgaptmp_003054"
     CDS             3878..4462
                     /gene="traP"
                     /locus_tag="ACHOQQ_RS15270"
                     /old_locus_tag="pgaptmp_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HGU4471270.1"
                     /translation="MANNTSSSQACHTARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEIDFIRQTAPAGQFPVRECLPSLPDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYLVRPGKMKCIRVVTADEHLKEV
                     YTEDASLPGKIRKCHAYFPDNRTNRNNGDKNEHA"
     gene            4449..4643
                     /locus_tag="ACHOQQ_RS15275"
                     /old_locus_tag="pgaptmp_003055"
     CDS             4449..4643
                     /locus_tag="ACHOQQ_RS15275"
                     /old_locus_tag="pgaptmp_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HGU4471271.1"
                     /translation="MNMRNIHVITALSVPDKRISDDFMHAVLSNCTTGVLPAPEKFGS
                     ESLPDNFTMTAVGVMKNSEI"
     gene            4655..4906
                     /gene="trbG"
                     /locus_tag="ACHOQQ_RS15280"
                     /old_locus_tag="pgaptmp_003056"
     CDS             4655..4906
                     /gene="trbG"
                     /locus_tag="ACHOQQ_RS15280"
                     /old_locus_tag="pgaptmp_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HGU4471272.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            4903..5418
                     /gene="traV"
                     /locus_tag="ACHOQQ_RS15285"
                     /old_locus_tag="pgaptmp_003057"
     CDS             4903..5418
                     /gene="traV"
                     /locus_tag="ACHOQQ_RS15285"
                     /old_locus_tag="pgaptmp_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HGU4471273.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLQRLAEGNFRTMPVQTVTATSPSGSRPAVTALPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVIPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            5553..5774
                     /gene="traR"
                     /locus_tag="ACHOQQ_RS15290"
                     /old_locus_tag="pgaptmp_003058"
     CDS             5553..5774
                     /gene="traR"
                     /locus_tag="ACHOQQ_RS15290"
                     /old_locus_tag="pgaptmp_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HGU4471274.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            5767..6240
                     /locus_tag="ACHOQQ_RS15295"
                     /old_locus_tag="pgaptmp_003059"
     CDS             5767..6240
                     /locus_tag="ACHOQQ_RS15295"
                     /old_locus_tag="pgaptmp_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471275.1"
                     /translation="MHKSENEKIDSILDEHQQIYRRHNRIDNILAFFACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            6320..6538
                     /locus_tag="ACHOQQ_RS15300"
                     /old_locus_tag="pgaptmp_003060"
     CDS             6320..6538
                     /locus_tag="ACHOQQ_RS15300"
                     /old_locus_tag="pgaptmp_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471276.1"
                     /translation="MHTFIFLVLMKNVGGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGGGL"
     gene            6566..6913
                     /locus_tag="ACHOQQ_RS15305"
                     /old_locus_tag="pgaptmp_003061"
     CDS             6566..6913
                     /locus_tag="ACHOQQ_RS15305"
                     /old_locus_tag="pgaptmp_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471277.1"
                     /translation="MKSIYLKYFLSFVFVGVMAMIVCIPFYIIYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTFGKANKIASECRKRNEMEEARRVRENELNKIREKQ
                     LQALSDADSAKER"
     gene            7044..9674
                     /gene="traC"
                     /locus_tag="ACHOQQ_RS15310"
                     /old_locus_tag="pgaptmp_003062"
     CDS             7044..9674
                     /gene="traC"
                     /locus_tag="ACHOQQ_RS15310"
                     /old_locus_tag="pgaptmp_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HGU4471278.1"
                     /translation="MSNNPLEVVTQAVNSLLTALKLPDESAQANQILGEMNFPQFSRL
                     LPYRDYNQESGLFMNDSTMGFMLEAIPINGANETIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGERIEYGLREFSWSGEQAERFNAITRAYYMKAAETLFPLPEGLNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLTRQNRARIDDVVDFLKNARDNDQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            9671..10057
                     /gene="trbI"
                     /locus_tag="ACHOQQ_RS15315"
                     /old_locus_tag="pgaptmp_003063"
     CDS             9671..10057
                     /gene="trbI"
                     /locus_tag="ACHOQQ_RS15315"
                     /old_locus_tag="pgaptmp_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HGU4471279.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEAQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            10054..10686
                     /gene="traW"
                     /locus_tag="ACHOQQ_RS15320"
                     /old_locus_tag="pgaptmp_003064"
     CDS             10054..10686
                     /gene="traW"
                     /locus_tag="ACHOQQ_RS15320"
                     /old_locus_tag="pgaptmp_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HGU4471280.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEADMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            10683..11675
                     /gene="traU"
                     /locus_tag="ACHOQQ_RS15325"
                     /old_locus_tag="pgaptmp_003065"
     CDS             10683..11675
                     /gene="traU"
                     /locus_tag="ACHOQQ_RS15325"
                     /old_locus_tag="pgaptmp_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HGU4471281.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWNCIFP
                     LSLGSTKVSQGKVPDTINPSMPIQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGITIPELGKTGQGTAKKDDKQVNGAFYHVHWYKYPLTYWLSIITSAGCLMGGDMD
                     IGYISEIDPTWTDSSLTTILNPEAVIFANPIAQGACAVDAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNSIGVDVAVCYEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            11887..>12122
                     /locus_tag="ACHOQQ_RS15330"
                     /old_locus_tag="pgaptmp_003066"
     CDS             11887..>12122
                     /locus_tag="ACHOQQ_RS15330"
                     /old_locus_tag="pgaptmp_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471282.1"
                     /translation="MKAVEVYGRHSDVVMSLERQTDLLEAAGNVLKQRWFYRPWGSSK
                     ASVMLTGILVIPVFLVLSGLLSAGYPGLLCITVSG"
BASE COUNT         3033 a         2809 c         3359 g         2921 t
ORIGIN      
        1 agtggttgcg atatgctata atttctgatg tgttatttta tctatatcaa tacttcgcta
       61 gtaatattaa caaccttcac cgcaaagaaa tgtaagtatt cccggcacta aaagtgccgg
      121 tttttgttaa cgtaatttac tcttttgttg tttgttttgt gtgcgtctta tgtaaatatt
      181 ttgactaaat ctcttttatg tacgggaggt gtggtgatca aaggtgcttt aagagctgct
      241 ccaagtagaa aggttttact gacccttgat gaggcaacca ataaattatt acttggagcc
      301 agagagcgca gtggaagaac gaaaacaaat gaagcattgg tgaggttgca ggatcatttg
      361 caacgttatc cagattttta taacgaagag atattcaggg aggtgacaga agagagagaa
      421 tcaactttta aagaacttta gttttaatct ttttacatac aatccgaggt aattgatgaa
      481 tacttctgtt agtgttccgg gggcttttac gcctgttaaa aagaaatcat cttttttctc
      541 taaactgaac ctgcttcgcc tggttcgtgc tgtgatcccg gcagctgtgc tgatgatgtt
      601 cttccctcag atggccctgg ctgctggcgg tcaggacctc atgcagtccg ggaatgacac
      661 agtcaaagcc acctttggta aggactccag tattgttaaa tgggtcgttc tggctgaagt
      721 gctggtcggg gcggtcatgt acatgatgac caaaaacgtc aagttcctgg ccggttttgc
      781 catcatctct gtatttattg ctgtaggtat gaccgtcgtt ggcctctgac aggaaataaa
      841 acgatgtcgg gagacgagaa taaacttaag aaatatcgtt tcccggaaac actgaccaac
      901 cagagccgct ggtttggcct gccactggat gaactgatcc ccgcagcgat ctgtattggc
      961 tggggtatca caacatcgaa atatctgttc ggtattggtg cggcggttct ggtttatttc
     1021 gggataaaaa agctgaaaaa agggcggggc agcttctggt tacgggatct tatttactgg
     1081 tatatgccaa cagtcctgct gcgcggtatt tttcataatg ttcctgattc gtgtttccgg
     1141 cagtggatta aatagaactg atacctggat tgttatatgg aacacggtgc ccgcttaagt
     1201 accagtcgtg tactggccat tgcttttata tttatgtccc tgctcattgt tctcagcctg
     1261 actgttaacg tgattcaggg ggtgaataat taccgccttc agaatgaaca gcgtactgcc
     1321 gtgacaccga tgggatttaa tatgcccttt gcggtatcac agaacagtgc tgatgcctct
     1381 tttttgcagc agatggcgct ggcgtttatt gccctgcgcc tgaatgtctc accggaaatc
     1441 gtcgatgctt ctcaccaggc acttctgcag tatatccgtc cgggcgcgca gaataagatg
     1501 aaagtgattc tggccgaaga agcaaagctc atcaaaaaag ataatgtgaa ttctgctttt
     1561 ttccagacca gtgttcgtgt ctggccacaa tatggtcgtg tggaaatccg tgggattcgg
     1621 aaaacatgga ttggtaactc cgaacctttc actgatatca aacattacat tcttattctg
     1681 aaacgggaaa acggggtgac ctggctggat aatttcgggg aaacagacga tgagaaaaaa
     1741 taagacagta ataatatccg cctgcctgct tttttccggc agcgtgctgg caggaaacgg
     1801 cacactggca cctacagtgg ttccgatggt caatggcggt caggcaagta ttgccatcag
     1861 taataccagc ccgaatctgt ttaccgtccc cggtgaccgg attatcgccg tgaacagtct
     1921 ggatggtgcc ctgaccaata atgagcagac tgcgtccggt ggtgtggtgg tggccaccgt
     1981 caacaaaaag ccttttacgt tcattctgga aacagaacgt ggtcttaatc tttccattca
     2041 ggccgttccc cgtgaaggcg ccgggcgtac cattcagctg gtcagcgatt tgcgcggaac
     2101 aggagaaact gccggtgcat gggaaacatc cacgccttac gaatccctgc tggtttccat
     2161 cagtcaggcg gttcgtgccg gacaactgcc agaaggctgg tatcagatcc ccgtgacaaa
     2221 ggaaaccctg caggcccctg ccggaatgtc cgcaaaagct gaagccgtat ggacggggaa
     2281 tcacctgaag atggtgcgct ttaccgtgga aaataaaacg ctgtctgcgc tgaatatccg
     2341 ggaaagtgac ttctggcagc cgggaacccg ggcggtgatg ttcagccagc ctgtcagcca
     2401 gttactggtc gctgcccgta tggatgtgta cgtcattcgt gacggggagg gcaactgatg
     2461 gccagtatca acaccattgt gaaacgcaag cagttcctgt ggctggggat tgtgattgtc
     2521 ggatcggtat cagcaattgg tggtgcgctt tatctgtctg atatggatat gtccgggaac
     2581 actgatgccg ttgcagggca ggagcctgtg ccggatatga ccggtgttgt ggatacgacc
     2641 tttgatgaca aggtgcgtca gcatgccacc acagagatgc aggtgacggc ggcacagatg
     2701 cagaagcagt atgaggaaat ccgtcgtgag atggatgttc tgaacaaaca gcgtggtgat
     2761 gaccagcgtc gcattgaaaa gctggggcag gagaatgctg cgctggcaga gcaggtgaaa
     2821 gccctgggcg cgaatcccgt tacagtgacc ggagagcctg tgccacagat gccggcatcc
     2881 cctcccggtc cggaggggga gcctcagccc ggtaatgcgc ccgttgcttt tccaccgcag
     2941 ggggcaggtg cagtgccacc gcccaccgcg ttctatccgg gcaacggagc cacgccgccg
     3001 ccacaggtga cgtaccagtc tgtgccggtg ccgaaccgga tacagcgtaa aacgttcagc
     3061 tatgatgctg gcacaaagca aactccgtcc ctgccgtaca ttccgtcagg cagttttgcg
     3121 aaagccattc tgattgaagg ggcggatgcc aatgcttctg tcaccggtaa tgaatccacg
     3181 gtgccgatgc agctgcgtat caccggtctg gtggaaatgc cgaacagcaa aacctatgac
     3241 gcaacgggat gctttgttgg gctggaagcc tggggggatg tgtccagtga gcgtgccatc
     3301 gtgcgtaccc gcaatatcag ctgcctgaag gagggtaaaa ccatcgatat gccggttaaa
     3361 gggcatgtca gcttccgggg taaaaacggt atcaagggtg aagtggtgat gcgtaacggc
     3421 aaaatcctcg gctgggcatg gggcgcggga tttgttgacg gtatcggtca gggaatggag
     3481 cgcgcttccc agccggctgt cgggctgggt gccactgccg cttacggggc cggcgatgtc
     3541 ctgaaaatgg gtatcggtgg cggtgcatcg aaagcggcac agacactcag tgactactac
     3601 atcaaacgcg ctgagcagta tcacccggtg atacccatcg gggcgggtaa tgaagtgacg
     3661 gtggtgttcc aggacggttt ccagctgaaa accgtggaag agatggcgct ggagcaggct
     3721 caggggcagg tggaagagag taacccggga agtcccgtgc cgaatccgcc atccgcagag
     3781 agtcacctga atggtctgaa taccgaccag atgctgaagc aactgggcga cctgaatccg
     3841 cagcagttta tggccggtgg tcaggaaggg ggcaacgatg gcaaataata cgtcgtccag
     3901 tcaggcgtgc cataccgccc ggtatgttgt cgcccgggtt ctgcgtggac tgttctggtg
     3961 tctgaaatac accgtcattc tgccactggc cacaatggcc ctgatggcgt tatttgtttt
     4021 atggaaggac aacaccacac cagggaaatt actggttcag gaaattgact tcataagaca
     4081 aacagcccct gccgggcagt ttcctgtaag agaatgtttg ccttctctgc cagatcttcc
     4141 aggagcagaa gagaaaatct gccgttaccg tgctgctgat gctgcagatt atgtcaggga
     4201 gactgaccgg tcactgatgc aactggtcac cgcattatgg gcaacactgg cactgatgta
     4261 tgtctcgctg gcagcaatca ccgggaaata tctggtgcga cccggaaaaa tgaaatgtat
     4321 ccgggtggta accgcagatg aacatctgaa ggaagtttat acggaagacg cttccctgcc
     4381 gggaaaaatc aggaaatgcc atgcttattt ccctgataac agaacaaaca gaaacaacgg
     4441 agataaaaat gaacatgcgt aatattcatg ttattacggc cctctctgta ccggataaac
     4501 gcatatcaga tgattttatg catgctgttc tcagtaactg caccacagga gtacttcctg
     4561 caccggagaa attcggttct gagtcattgc cggacaattt tactatgact gctgtcggtg
     4621 tgatgaagaa ttcagaaata taaggaaccc ttcgatgaat aagctggttt ctgatggcag
     4681 cgtcaaaaaa ataaattatc cggtgctgta tgagtccgga ataacacctc cgttatgtga
     4741 agtcagtgca ccagaacctg atgcgggtgg gaaacgcatt gtggcatatg tttataaatc
     4801 atcacggaat acggtttttg aaaatcctga cattgtaaaa acctgtacgg tcagggattt
     4861 gaagaatgat tttgtgaact gcgatgagaa aggtgaggga caatgaaaca gatttcttta
     4921 tttattccgc tgctggggac gttattactt tccggctgtg ccggaacaaa ctccgacttt
     4981 gagtgtgatg ccaccacatc cgatacctgt atgacgatgg agcaggccaa tgagaaggcc
     5041 aaaaaactgg agcagtcctc agaagcaaag ccggttgcgg catcactgca gcgcctggct
     5101 gaagggaact tccggacaat gccggtgcag accgtcaccg cgacctctcc gtccggcagc
     5161 agacctgcgg tgacggcact tccggagcag aaactgctgg caccacgtcc gctgtttacc
     5221 gctgcccggg aagtgaaaac ggttgttccg gtcagttcag ttgcaccggt aatacctccc
     5281 cgtccgctaa gaacgggtga gcagacggca gcattatgga tagcgcctta tattgataat
     5341 caggatgttt atcatcagcc atccagtgtg ttttttgtta ttaaaccgtc tgcgtgggga
     5401 aaaccacgta ttaattaaca ggccctgaag ttgtatatca gccggcaatg ccggcgggct
     5461 ttctgtattc aaaatctgcc tgtaaaacga gtgccctgtg cgtgaaaagg gatgggcggt
     5521 agcgtgtcag aacatgtcgg gaggaatatt ccgtgagtga tgaagccgat gaagcatatt
     5581 cagtaacaga acaactgacc atgacaggaa taaaccggat acgccagaaa ataaatgttc
     5641 atggtattcc tgtttatctc tgtgaagcat gcggaaatcc tattccggaa gcccggcgga
     5701 aaatatttcc cggtgtgacg ttgtgcgttg aatgtcaggc gtatcaggaa aggcagagga
     5761 aacactatgc ataaatcaga aaatgaaaaa atagattcta ttctggatga acatcaacag
     5821 atatatcgca gacataaccg aatagataac atcctggcgt tttttgcgtg tattgcatgg
     5881 attccctgta ttattgctgc gttcggcgtt gatggtatat atctgaaaat cctgtttgcg
     5941 gtccttgcct gctgtggtgc ggtatgcttt tttattttct tcttcactct cctgccggaa
     6001 tcactcatgg tcctcagccg tcagagcctg cttcagctta tgcgtctgac tgaagacgta
     6061 ccggatgcga gacaggaact gcttaaccgt ctgctgtccg gtaaaaaact gactggtcgg
     6121 gatgaaaagg atatccggcg tctctggcag gagaaagtgg atgcaatgca ggagtctgcc
     6181 acccgtcagc gtgaacagga taccatccgg aagtttactg aagggaataa atccgaatga
     6241 aaatctgctg tgttttataa cctcataagt taactgaagg agtattcagt gattaatatt
     6301 tcactggagg taataaagaa tgcatacctt tatttttctt gtgctgatga aaaatgttgg
     6361 cggtggcgga agctggaatg ttacccccat gccaaatacg gacgtatgcg tgcaggttct
     6421 tcggacaatc gaagcacatg gaggcagggg atttggaaat acaggggagt gtattgaagt
     6481 taaaactaat gaacctttga ataacgtgat ggaaacactg aatggtggtg ggctatgagt
     6541 gcttactgct tcgaaggaaa atgaaatgaa gtcgatatat ttaaaatatt ttctgtcttt
     6601 tgtatttgtt ggtgttatgg ctatgattgt atgtattcct ttttacatca tttaccttgc
     6661 gcaacaacct gcaacaccag aacaactgac agagatttta caggaaactc cctgtgcagc
     6721 agaagcattt caggagacac ttaattatca gtctgaacct ttaacatttg gtaaggcaaa
     6781 taaaatcgca tcagaatgca gaaaaagaaa tgaaatggaa gaggcgcgac gggtaagaga
     6841 aaacgagctc aataagatac gcgaaaaaca acttcaggca ttaagtgatg ctgattcagc
     6901 aaaggaacgt taattgtaat cctggtttcc tgttaaaagc ctccatctct tatttgtgct
     6961 gtggtttcag aaatggcact gcgtgatata tccacgccac agactgatat tttccttttt
     7021 taaacgtact aaggaatact catatgagta ataacccact tgaggtcgtc actcaggcgg
     7081 tcaattcact gctgaccgca cttaaattac cggatgaatc tgctcaggct aatcagatcc
     7141 tcggtgagat gaacttcccg cagttcagcc gtctgctgcc ataccgtgat tacaaccagg
     7201 aatccggtct gttcatgaat gactccacaa tgggctttat gctggaagcc attcccatca
     7261 atggtgcgaa tgagaccatt gtggaggctc tcgaccatat gctgcgcacc aaactgccgc
     7321 gcggtattcc gctgtgtatc catctgatgt ccagccagct ggttggcgag cggattgagt
     7381 acgggctgcg tgagttctcc tggtcaggtg aacaggcaga acggtttaac gccattaccc
     7441 gggcctatta catgaaagcc gcagaaacac tgtttccgct gccggaaggg ttgaacctgc
     7501 ctctgaccct gcgccattac cgggtgttta tctcgtactg ttctccctcg aagaaaaaaa
     7561 gccgggccga cattctggaa atggaaaacc tggtgaaaat catccgggcg tctctgcagg
     7621 gagcatatat caccacgcag acggtggatg cacaggcttt catcgatatt gtcggggaga
     7681 tgattaacca taacccggac tccctgtacc cgaaaagacg tcagctggac ccgtattctg
     7741 atttgaatta tcagtgcgtg gaggacagtt ttgacctgaa ggtccgggca gattacctga
     7801 cgctgggact gcgtgagaac ggcaggaaca gcactgcccg catcctgaat ttccatctgg
     7861 cccgtaaccc ggaaatcgcc ttcctgtgga acatggccga caactacagc aacctgctga
     7921 acccggaact gtccatctcc tgcccgttca ttctgacgct gaccctggtg gtggaagacc
     7981 aggtgaaaac ccacagcgaa gccaacctga agtacatgga cctggagaaa aagtcgaaga
     8041 cctcctatgc caaatggttt ccgtccgtgg agaaagaagc gaaggagtgg ggggaactgc
     8101 gtcagcggct gggctccggt cagtcttctg tcgtgtccta cttcctcaac atcacagcat
     8161 tctgcaagga caataatgaa acggcactgg aagtggagca ggatatcctg aacagcttcc
     8221 gtaaaaacgg ttttgaactg atttcaccgc gctttaacca catgcgcaat ttcctgacct
     8281 gcctgccctt tatggccggg aaagggttgt ttaaacaact gaaagaggcc ggagtggttc
     8341 agcgcgcaga gagctttaat gtggccaacc tgatgccgtt agtggcggat aaccccctga
     8401 cgccggcagg tctgctggca cccacctacc gtaaccagct ggcgtttatc gatattttct
     8461 tccgggggat gaataacacc aactacaaca tggcggtctg tggcacttcc ggggccggta
     8521 aaaccgggct gatacagcca cttatccgca gtgtgctgga ctccgggggt tttgctgtgg
     8581 tgttcgacat gggggatggc tacaagtccc tgtgtgaaaa catggggggc gtatatctgg
     8641 acggtgaaac cctgcgcttt aacccgtttg cgaacatcac cgatattgac cagtcagcgg
     8701 agcgtgtccg tgaccagctg tcggtgatgg ccagccccaa cggtaacctg gatgaagtgc
     8761 atgaaggtct gctgctgcag gcggtcaggg cctcctggct taccagacag aaccgggcgc
     8821 gtattgatga cgtggtggat ttcctgaaaa acgcccggga taacgaccag tatgccgagt
     8881 ccccgaccat ccgcagccgt ctggatgaaa tgatagtgct gctggaccag tacacagcca
     8941 acggtactta cggccagtat tttaactctg atgaaccgtc cctgcgggat gacgcaaaaa
     9001 tggtggtgct ggagctgggt ggactggaag accgtccgtc cctgctggtt gcggtgatgt
     9061 tcagccttat catttacatc gagaaccgga tgtaccgcac gccgcgtaac ctcaagaaac
     9121 tgaacgtcat tgatgagggg tggcgtctgc tggacttcaa aaatcacaag gtcggtgagt
     9181 ttattgagaa aggttaccgt accgcccgcc gtcatactgg tgcctatatc accatcacac
     9241 agaacatcgt cgactttgac tctgacaagg cctccagtgc ggcccgtgcg gcatggggta
     9301 actcctccta caaaattatc ctcaaacaga gtgcgaagga gttcgcgaaa tataaccagc
     9361 tgtatccgga ccagttcctg ccactgcagc gcgacatgat tggtaagttt ggtgcggcca
     9421 aagaccagtg gttcagttcc ttcctgctgc aggtggaaaa ccactcctcc tggcaccgtc
     9481 tgtttgtgga cccgctaagc cgtgccatgt acagctctga cggcccggat tttgagtttg
     9541 tgcagcagaa acgtaaagag gggctgagta ttcatgaggc ggtgtggcag ctggcgtgga
     9601 agaagtcagg gccggagatg gcttcgctgg aagcatggct ggaagaacat gagaaataca
     9661 ggagtgttgc atgaccacaa cgcaaaaaac gactgatgtg acagcccgtc gacgcagcca
     9721 ctggtggtgg acggtgccgg gatgcctggc aatggtgtta ctgaacgcag ccgtcagtta
     9781 cggcattgtc agactgaaca cgccggtaac cgtcgccttc aacatgaaac agacagtgga
     9841 tgcgtttttt gacagcgcca gccagaaaaa actgtcagaa gcgcagtcga aggcgctggc
     9901 tgaccgtttt aacgcggcac tggaggccag tctgcagacg tggcagcaga agcaccacgc
     9961 tgtcattctg gtgtcgcctg ccgtggtaca gggggcaccg gatatcaccc gtgaaatcca
    10021 gcaggatatt gcccggcgaa tgagggcaga accatgagat gccgggggct gattgccctg
    10081 ctggtatggg ggcagagtgt ggctgcagct gatcttggca cctgggggga tttgtggccg
    10141 gtaaaggagg cggacatgct gacggtgatt atgcagcgtc tgacagctct ggagcagtcc
    10201 ggagagatgg gccggaaaat ggatgcgttt aaggagcggg tgatccgcaa cagcctgcgg
    10261 ccccctgccg tgcccggtat cggtcggacg gagaaataca gcagccgcct gtttgacccg
    10321 tctgtcagac tggctgcgga tatccgggat aacgaaggtc gtatcttcgc ccgccagggg
    10381 gaggtgatga acccgctgca gtatgtgcct tttaaccaga cactgtattt catcaacggt
    10441 gatgatcctg agcagattga atggatgaag cgccagacgc ctcccacact ggagagcaaa
    10501 attatcctgg tgcagggcag tatcccggag atgcagaagg ctctggacag tcgtgtttac
    10561 tttgatcaga acggcgtgat ctgccagcgt ctcggcattg atcaggtgcc ggcacgggtg
    10621 agtgctgtca gtggcgatcg tttcctgaag gtggagttta ttccggtcgg ggagggaggg
    10681 aaatgaaacg gtgtatgtgg ttgtgtgtgg ttttgctgct gactggccat gttcccgcag
    10741 cctcagcgga cgctgcctgt gaagggcggt ttgttaaccc gatcactgat atctgctgga
    10801 actgcatttt tccactgtcg ctgggcagca caaaagtgag tcaggggaag gtgccggata
    10861 caataaaccc gtcaatgccg atccagttct gtcctgcgcc accgcctgta tttaaacgta
    10921 tcgggctggc tattggttac tgggagccga tggcactgac ggatgtgacc cgcagtccgg
    10981 gatgtatggt aaatcttgga ataacaatcc cggagctggg gaaaaccggg cagggaacgg
    11041 ccaaaaaaga cgacaagcag gtgaacgggg cattttacca tgttcactgg tacaaatacc
    11101 cgctgacata ctggctgagt attatcacct cagcaggatg tctgatgggc ggcgatatgg
    11161 atatcgggta catctcagaa attgacccta cctggacaga cagcagtctg accaccattc
    11221 tcaacccgga agcggtcatc tttgcaaatc cgatagcaca gggagcctgt gcggtggatg
    11281 ccattgccag tgcctttaac aagccgctgg atatcctgtt ctggtgtgcc ggttcgcagg
    11341 gcagtatgta tccgtttaat ggctgggtga gcaatgaatc cagtccgttg cagtcatccc
    11401 tgctggtcag tgaacggatg gcgtttaagc tccaccgtca gggaatgata atgaacagca
    11461 tcggcgtgga tgtggctgtc tgttatgaat atccgtcgcc catacttccc aaagagcgct
    11521 ggcgttatca gatggtgaat atgtacccgg acagcggaca gtgccatccg ttcggacgca
    11581 gtgtgatgcg ctgggagacg ggaaaaaatc cgcccaacac aaagaaaaac ttcggttacc
    11641 tgatgtggcg taaacgtaac tgtgtgttcc tgtaatcggg aaaatgtgat gcgcaacagt
    11701 aaagtgcaac ttgtatcaat cctcagacag gtcagtcttt cactgaacac ggagccatta
    11761 cgacagttta tctctctgag ggagattgca gaagagacgg atcacgtcgc tgctcgtttg
    11821 tccggaggaa aacgggtaac acctgcacaa atttatgaat tgtgtgcaca gttatggatg
    11881 gccaggatga aggcagttga agtttatggg cgacattctg atgtggtgat gtctctggaa
    11941 agacagactg atttacttga ggctgcaggc aacgtattaa aacaacgctg gttttaccgt
    12001 ccgtggggat catcaaaagc atctgtgatg ctgacaggta tactggtgat ccccgtattt
    12061 ctggttttat ccggattgtt gagtgcaggg tatcccgggt tattatgtat taccgtatcc
    12121 gg
//
LOCUS       DAZKFU020000136        12003 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZKFU020000136 DAZKFU020000000
VERSION     DAZKFU020000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.137"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            165..707
                     /gene="yfaZ"
                     /locus_tag="ACHOQQ_RS15335"
                     /old_locus_tag="pgaptmp_003067"
     CDS             165..707
                     /gene="yfaZ"
                     /locus_tag="ACHOQQ_RS15335"
                     /old_locus_tag="pgaptmp_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGU4471283.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQVGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            807..2009
                     /gene="yfaY"
                     /locus_tag="ACHOQQ_RS15340"
                     /old_locus_tag="pgaptmp_003068"
     CDS             807..2009
                     /gene="yfaY"
                     /locus_tag="ACHOQQ_RS15340"
                     /old_locus_tag="pgaptmp_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGU4471284.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2229..3011
                     /gene="yfaX"
                     /locus_tag="ACHOQQ_RS15345"
                     /old_locus_tag="pgaptmp_003069"
     CDS             2229..3011
                     /gene="yfaX"
                     /locus_tag="ACHOQQ_RS15345"
                     /old_locus_tag="pgaptmp_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HGU4471285.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3026..4231
                     /gene="rhmD"
                     /locus_tag="ACHOQQ_RS15350"
                     /old_locus_tag="pgaptmp_003070"
     CDS             3026..4231
                     /gene="rhmD"
                     /locus_tag="ACHOQQ_RS15350"
                     /old_locus_tag="pgaptmp_003070"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HGU4471286.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4288..5577
                     /gene="yfaV"
                     /locus_tag="ACHOQQ_RS15355"
                     /old_locus_tag="pgaptmp_003071"
     CDS             4288..5577
                     /gene="yfaV"
                     /locus_tag="ACHOQQ_RS15355"
                     /old_locus_tag="pgaptmp_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4471287.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5595..6398
                     /gene="yfaU"
                     /locus_tag="ACHOQQ_RS15360"
                     /old_locus_tag="pgaptmp_003072"
     CDS             5595..6398
                     /gene="yfaU"
                     /locus_tag="ACHOQQ_RS15360"
                     /old_locus_tag="pgaptmp_003072"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HGU4471288.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVDGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6439..6672)
                     /locus_tag="ACHOQQ_RS15365"
                     /old_locus_tag="pgaptmp_003073"
                     /pseudo
     CDS             complement(6439..6672)
                     /locus_tag="ACHOQQ_RS15365"
                     /old_locus_tag="pgaptmp_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6673..7644)
                     /gene="rpnE"
                     /locus_tag="ACHOQQ_RS15370"
                     /old_locus_tag="pgaptmp_003074"
     CDS             complement(6673..7644)
                     /gene="rpnE"
                     /locus_tag="ACHOQQ_RS15370"
                     /old_locus_tag="pgaptmp_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGU4471289.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSLLQKERLMTIAERLRQEGHQIGWQEGKLEGLQQGKLEGLQ
                     KGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(7837..9027)
                     /gene="glpC"
                     /locus_tag="ACHOQQ_RS15375"
                     /old_locus_tag="pgaptmp_003075"
     CDS             complement(7837..9027)
                     /gene="glpC"
                     /locus_tag="ACHOQQ_RS15375"
                     /old_locus_tag="pgaptmp_003075"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGU4471290.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9024..10283)
                     /gene="glpB"
                     /locus_tag="ACHOQQ_RS15380"
                     /old_locus_tag="pgaptmp_003076"
     CDS             complement(9024..10283)
                     /gene="glpB"
                     /locus_tag="ACHOQQ_RS15380"
                     /old_locus_tag="pgaptmp_003076"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGU4471291.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10273..11901)
                     /gene="glpA"
                     /locus_tag="ACHOQQ_RS15385"
                     /old_locus_tag="pgaptmp_003077"
     CDS             complement(10273..11901)
                     /gene="glpA"
                     /locus_tag="ACHOQQ_RS15385"
                     /old_locus_tag="pgaptmp_003077"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGU4471292.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVHVRNHLTGETQSLHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAQTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
BASE COUNT         2683 a         3067 c         3301 g         2952 t
ORIGIN      
        1 gtatcggtaa agtgtgttta ttttttcggc gtttaattat ttaagaagta tttagttaca
       61 ataagtgact agatcccttt tttcgctcat tgaatcctct gcgccaggca gtacaatccc
      121 gcagctttgg gaattttgat taattgcttg tgggaaaggg gataatgaaa aaaattgcgc
      181 tagcaggtct ggccggaatg ctgttggttt ctgcatcggt caatgcaatg agcatcagcg
      241 gccaggtggg taaagaatac accaatattg gtgtcggttt tgggactgaa tcgacgggcc
      301 tggctttaag cggtaactgg acacataacg acgacgacgg tgacgtcgcg ggcgtggggc
      361 tggggttgaa tctgcctctc gggccgttaa tggcgaccgt tggcggaaaa ggcgtttaca
      421 ccaacccgaa ttacggcgat gaaggttatg ccgcagcggt aggaggtggt ttgcagtgga
      481 aaattggcaa cagcttccgt ttgtttggcg agtattacta ctctccggat tcactctcca
      541 gcggtattaa aagttatgag gaagcgaatg ctggcgcgcg ttacaccatt atgcgtccag
      601 tcagtattga ggcgggttat cgctacctga atctgtcggg taaagacggt aaccgcgaca
      661 acgccgtggc tgacggcccg tatgttgggg ttaacgccag tttctgatcc cttccggcgc
      721 ggcattctgt cgcgccattc agtttcttac tacgccttat ctccctttgc gttatagtga
      781 ttcgataaca aaagcaggag ataaccatgt taaaagtgga aatgctatcc accggggatg
      841 aagtgttaca cgggcaaatc gttgacacta acgctgcctg gctggccgat tttttctttc
      901 atcaggggtt gccattatct cgccgcaata cggtggggga taaccttgat gacttagtca
      961 ccattcttcg cgaacgtagt cagcacgccg atgtgctgat cgttaacggc gggctggggc
     1021 cgaccagtga tgatttaagc gcactcgccg ctgcgacagc aaaaggtgaa ggcctggtgc
     1081 tgcatgaagc ctggctcaaa gagatggaac gctatttcca cgaacgtgga cgagtaatgg
     1141 caccgagcaa ccgtaaacaa gcggagctgc ctgccagtgc tgaatttatc aataacccgg
     1201 taggcaccgc ctgtggtttt gccgtgcagc ttaatcgttg cctgatgttc tttactcccg
     1261 gcgtaccgtc agaatttaag gtgatggtcg agcacgaaat cctgccgcgt ctgcgcgagc
     1321 gtttttcttt accacagccg ccggtttgcc tgcgtttgac tacctttggt cgttcggaaa
     1381 gcgatctggc acaaagcctg gacactctac aactgccgcc gggcgtaaca atgggctatc
     1441 gctcctcaat gccgatcatc gaactgaaac tcaccggacc ggcaagcgag caacaggcga
     1501 tggaaaaact gtggctggac gttaaacgtg ttgccggaca gagcgtgatt ttcgaaggca
     1561 ccgaaggact gcccgcgcag atcagtcgcg aattgcaaaa ccgccagttc agcctgacgt
     1621 tgagcgagca attcaccggt ggtttattgg ctttgcaact ttctcgcgca ggtgctccat
     1681 tgctggcgtg tgaagtggtt ccttcacagg aggaaaccct ggcgcaaact gcgcactgga
     1741 ttacagaacg gcgggccaac cattttgccg ggctggcact ggctgtttcg ggtttcgaga
     1801 acgagcatct caactttgcg ctagccacgc cagacggcac tttcgctctg cgtgtgcgtt
     1861 tcagcactac acgctacagc ctggctatcc gtcaggaagt gtgcgcaatg atggcactga
     1921 atatgctgcg ccgttggtta aacggccagg acatcgccag tgagcatggc tggattgagg
     1981 ttgttgagtc catgacctta tccgtctgat tccgccactc tgtgccggtt tactgcaccg
     2041 gcacattccc cctccggcaa aatttgtcca acctgttgta ctcaataaga aaaaatccgt
     2101 gcgctgattc acaaatttgt gcgtaatttc tccagtccgt tggacgagga gcgggatttg
     2161 atgcgcttca caattttact ccccctgagc aacgagactg attccagtat ctaagactgg
     2221 agtccagtat gttggaatca agcaaagttc ccgccctgac acgggctatt gatattctca
     2281 atttgattgc ccgcattggc ccctgtaatg ctgcgacgat cattgacaca ttggggatcc
     2341 ctaaaagcac ggcctatttg ctgcttaatg aactcaggcg tcagcgtttt cttagccttg
     2401 atcaccagga aaatttttgt ttgtggacca ggctggtgga gctttccggt catgcgttaa
     2461 gcaagatgga tctccgggaa ctggctcgac cgcgtttgac gcaactgatg gataccaccg
     2521 ggctgttatg tcacctgggg attattgata acggcagcgc ttattacatt ttgaaagtgg
     2581 agtcatcagc cactatcagc gtgcgttcac atgaaggaaa aagcctttcg ctttatcgct
     2641 ccggaatagg taaatgcctg cttgcctggc aacctgcaac tgtacagcaa agtattatcg
     2701 aagggctggt ctgggagcag gcgacaccca caacgattac tcatccacag caacttcacg
     2761 aagaactggc gcgtattcgc cgtcagggct ggagctatga caacggcgaa gattacgccg
     2821 atgtccgttg tgtcgctgcg ccggtgttta acgccaataa cgagcttacc gctgcgatct
     2881 cggtggtcgg tacccgctta caaatcaatg aagaataccg tgattatctg gcgggcaagg
     2941 ccattgcctg cgccagggat atttcacgtc tgttgggatg gaaaagtccc tttgacttac
     3001 aagcctcata acaatggaga acatcatgac cctaccaaaa attaaacagg ttcgcgcctg
     3061 gtttactggc ggtgcgacag cagaaaaagg cgctggcggc ggtgattatc acgaccaggg
     3121 ggcgaatcac tggatagacg atcatattgc caccccgatg agtaaatacc gcgattacga
     3181 gcagtcacgc cagtcatttg gcattaacgt tcttggcacg ttggttgttg aagtcgaagc
     3241 agaaaacggc cagaccggat tcgccgtttc gacagccggt gaaatgggct gttttatcgt
     3301 cgaaaaacac cttaaccgtt tcattgaggg taaatgtgtc agtgatatca aactgatcca
     3361 tgatcaaatg ctcaatgcca ccctgtatta ctccggctct ggtggcctgg tgatgaacac
     3421 gatttcctgt gtcgatctgg ctctgtggga tctgttcggc aaagtggtcg ggcttccggt
     3481 ttataaactt ttaggcggcg ctgttcgtga tgagattcag ttctacgcca caggtgcgcg
     3541 tccggatctg gcaaaagaga tgggctttat cggtggcaaa atgccgacgc actgggggcc
     3601 acatgatggc gatgcgggga tccgcaaaga tgccgctatg gtcgcggata tgcgtgaaaa
     3661 atgcggtgag gatttctggt taatgctcga ctgctggatg agtcaggacg tgaactatgc
     3721 gaccaaactg gcccacgctt gcgcgcccta taacctgaaa tggatcgaag agtgcctgcc
     3781 gccacagcag tatgaaggtt atcgcgaact gaaacgcaac gcgccagtcg gaatgatggt
     3841 caccagcggt gagcaccacg gcacactgca atcttttcgt acgctttcag aaaccggtat
     3901 cgacattatg cagccggatg ttggctggtg cggtggctta accacgctgg tggaaattgc
     3961 cgcaatcgcc aaatccaggg ggcaactggt ggtgccgcac ggttcgtctg tttactccca
     4021 ccatgcggtg atcaccttca ctaatacgcc attcagcgaa ttcctgatga ccagtccgga
     4081 ttgttcaacg atgcgtccgc agtttgaccc gattctgctc aatgagccgg ttccggtgaa
     4141 tggtcgtatt cataaatcag tgcttgataa acccggtttt ggcgtcgaac tcaatcgtga
     4201 ctgcaatttg aaacgcccct acagccacta atcactccgg gcgttgctgt gacaacggta
     4261 acgctcacat attctaagga gctttttatg agcaccgctt tgcttgacgc cgtggtgaag
     4321 aaaaaccgtg tgcgtttaat tccgtttatg ttggcgctgt atgtgctggc gtttctcgac
     4381 cgttcgaata tcggttttgc caaacagacc taccagattg ataccgggtt gagtaatgaa
     4441 gcttatgcgc tgggagcagg cattttcttt gtggtatatg cgtttctggg tgttccggcg
     4501 aatcttttga tgcgcaaact gggggccaga acctggattg gtacgacaac actgctgtgg
     4561 ggatttcttt cggcagccat ggcatgggcc gatactgaag cgaaatttct gatagttcgc
     4621 actctgcttg gtgctgcgga ggccggattt ttccctggta tgatttatct cacctcgcaa
     4681 tggtttccgc agcgtaatcg cgccagcatt atggggctgt tctatatggg cgcaccgctg
     4741 gcgttaacac tgggatcacc gctttccggc gcgctgctgg agatgcatgg atttatgggg
     4801 catcccggct ggttctggat gtttgtgatt gaaggattgt tggcagtcgg cgctggggta
     4861 ttcacattct tttggcttga tgacacaccg gagcaggcac gttttctgag taaacaagaa
     4921 aaaacgttgc ttatcaatca actggcaagt gaagaacaac agaaagtgac ttctcggctg
     4981 agcgatgcgc tgcgtaatgg gcgagtctgg caactggcga ttatctacct gaccattcag
     5041 gtggcggttt acggattaat tttcttcctg ccgacccagg ttgcggcatt gctgggaaca
     5101 aaagtgggct ttacagcgtc ggtggtcacc gccattccgt gggttgcggc cttgtttggg
     5161 acctggctta ttccgcgcta ttccgataaa accggcgaac ggcgtaatgt cgcagcgctg
     5221 acattactgg cggcgggcat tggtattggt ctgtccgggc tgctttctcc agtaatggcg
     5281 atcgtagcgc tgtgtgttgc agccatcggg tttattgccg tgcagccggt gttctggacg
     5341 atgccgacac aactgctttc cggtacggcg ctggctgcgg ggattggttt tgtaaacctg
     5401 tttggtgcag tgggcgggtt tattgccccg atcctgcgcg tgaaagcaga aacgttattt
     5461 tccagcgatg cggcgggatt actgacgctg gcagcggtgg cggtcatcgg ttcgctgatt
     5521 attttcactc tgcgtgtaaa tcgtactgtt gcgcagaccg acgtggcaca tcattaaata
     5581 ggttaaggaa cacgatgaac gcattattaa ccaatccctt taaagaacgt ttacgcaagg
     5641 gcgaagtgca aattggtctg tggttaagct caacaactgc ctatatggca gaaattgccg
     5701 ccacttccgg ttatgactgg ttgctgattg atggggagca cgcgccaaac accattcagg
     5761 atctttatca ccagctacag gcggtagcgc cctatgccag ccaacccgtg atccgtccgg
     5821 tggatggcag taagtcgctg attaaacaag tcctggatat tggcgcgcaa actctactga
     5881 tcccgatggt cgatactgcc gatcaggcac gtcaggtggt gtctgccacg cgctatcctc
     5941 cctacggtga acgtggtgtt ggggccagtg tggcacgggc tgcgcgctgg ggacgcattg
     6001 agaattacat ggcgcaagtc aacgattcgc tttgtctgtt agtgcaggtg gaaagtaaaa
     6061 cggcactgga taacctggac gagatcctcg acgtcgaagg gattgatggc gtgtttattg
     6121 gacctgcgga tctttctgcg tcgttgggct acccggataa cgccgggcac ccggaagtgc
     6181 agcgaattat tgaaaccagc attcggcgga tccgtgctgc gggtaaagcg gctggttttc
     6241 tggctgtggc tcctgatatg gcgcagcaat gcctggcgtg gggagcgaac tttgtcgctg
     6301 ttggcgttga cacgatgctc tacagcgatg ccctggatca acgactggcg atgtttaaat
     6361 caggcaaaaa tgggccacgc ataaaaggta gttattgata tcaaaggccc atggggatcg
     6421 gctgtgggcc tgtgttaatt agtggttatt cgctgccaga tcggcttcgc ttagctgggt
     6481 ggccgcgagc acctggtcac gatcaatgcc ctgttccagc atgcgcaagg caattttaat
     6541 agcttgttca tgcatgcctt cctgcaaacc ttctaattta ccttgctgca aacctactaa
     6601 tttaccttct tgccagccaa tttgatgtcc ttcctgccgt agccgttcag caatagtcat
     6661 caatctctcc ttttaatgac agttcggaat atcagcatca gtgagttggg ttgccgccag
     6721 cacaatctca cgttcaaagc cctgctccag catgcgcaag gcaattttaa tggcttgctc
     6781 atgcatgcct tcctgcaaac cttctaattt acctttctgc agaccctcca atttaccttg
     6841 ctgcaaacct tctaatttac cttcctgcca accaatttga tgtccttcct gccgtagccg
     6901 ttcagcaata gtcattaatc tctccttttg tagtagtgaa cgttcggcaa tctcctgaat
     6961 aaaacgggtg aaacgggagg tatcgccgca ttgcagcaga taattaaaca gtgtttgtag
     7021 ctggctgtca ttagtgaagc ctctaaccaa aagcgtggtg atcctgtcga ccataccgat
     7081 taaatcgcgg tcgcgaatat gcttttgaat cagttcaagc agagctatac gccgatgttg
     7141 catgatctca tcgtcaggta cgatggtaat atccaccaac ggaaaagctt cggtgtacaa
     7201 ctgccgggca agttgcggat cgtcaaactc atccagccag ttgagtgagt acgggtaggg
     7261 cgaggtttcg ccatgataaa acagcaacgg caccaccagc gggactctgt catagccttt
     7321 gtccaggtga cgctgcatgg cggcagtggc atagcgcatt agccgaaaag ccatattctt
     7381 ttctgcagag ctttgatgtt caatcacgca gtagatatag ccgtcaccgt cgctggtttc
     7441 cacggaccac aaaacatccg agtagtaagc gcgtaaactt ttttcaataa aactggtggg
     7501 ttccaggcgt aaggtttgca ggttgcaaag cttgcgcagc ggctctggta gatgtatttc
     7561 gagaaaatcc cgtgcggttt cgggtgtgaa cataaaggtt ttaaatactg catcatgcgg
     7621 ggaggaggtt gttgattcgg tcatggttgt ccgtcacctg atatttatga tgacgcgaca
     7681 ttacggttat cccaggcctt tgacatctgc cagtttcagc ctttgcgaga cgttttcaga
     7741 ggaattttca ctgcgttgca acgcatacat tgggcacgga atcgtgtacg caaaaatgcc
     7801 cggtaaccac atgcttaccg ggcatcagaa aggagtttaa gccagcgcct gggccagtag
     7861 cgtaatcgga tgttcgcagc gaagacttgt ggacatctca atctgccatt tacaggtttc
     7921 gcagtcggtg accaccagat ctgcgccgct ttcttctatc tggcggaaca gtggtgcgcc
     7981 gatggcttgt gaggtggggt agttctcttt tttgaaaccg taagtacccg caataccgca
     8041 gcactgggaa tccagcaccg ttaactcaag ccccgggatg ttacgcaaca gctccagggt
     8101 gtagagcgtc cagcccattt tttccatatg gcacggagtg tgataaacca ctttcagcgg
     8161 cagcggtttc agcggtaacg ttttgccttc gtccagcttg cgccacagcc agcgggtcgc
     8221 cagttcgata tgatcgcgca agcctttgtt gtcgacattc agcacttccg ggtactcgtc
     8281 gcgcagggcg aaagtacagg tggaggaggt ggcaatcacc ggaatgcctt ttactcccac
     8341 ggcttcgcgg atcgactcta cattcgtaat tgcctgtttg cgtgctttat cggtaaagcc
     8401 gttggcgatt agcggcacac cgcagcattt ttctttgctg agcagttgca cgccagtacc
     8461 catcgcgttg agcactttaa ttaaatcttt acctaactgc ggatggttgt agttaacgaa
     8521 gcagccgtgg aaaaaggcga cctggtcttt atactgcgcc tgctgggccg ccacgctgcg
     8581 ataccagcga cgaaacgtgc cgaaggagta tttcggtagc gtgcggcgat gatcgatttt
     8641 taacgccgca tcaagcaact gccgcaccgg tttcagcgag gtagcggtgt tgacgattgg
     8701 tgcgaacggc gtggaaacgc tacccatcag gtcggtatgg cttaaaacaa aattgcgcag
     8761 cgacgggcgc gtggtgtcat atttcgcccg tgcgcgctgg atgatatcgc caatcttcac
     8821 atcggacggg caggcgactt cgcaacgttt gcagttgatg caatatttca gtgcctcgtc
     8881 atacagtgcg ccatctttta aacgcagacg ctcgccatcc ggcccggctt gttttggccc
     8941 tggataaccg ggattcaccc ggctcactgg gcaggcggtg gtgcagacgg tgcacttaat
     9001 gcagttttcg aagctggtgt cattcattgt tggcctcctg cgcgttgggc aatctgttga
     9061 gcggcatgta aagcactgac ggcacaaaca ccgccgccgc atccctgggc gatgggatca
     9121 aatccgccca gcaccgaacc gatggcaaac aggttttcga tggtttgccc tgcctgcgac
     9181 gggcgtagcg tctgatcagt ggttacaccg aactgctgcc acggttgcgg cgcaaaaaaa
     9241 tctcccttat accattcacc ccgcgtggcg gtttgtagca catcaaggcc gagaatcggc
     9301 tctcgaatgc cgttacgttc cgctaccagt ccgccactaa agaaactgcc gctggcgaga
     9361 accgcgaaac gtggacgcag cgggatatcg gcgtgattgc gggtccagat ttcattcact
     9421 acgccatttt tacaggtcac ttttttcact tcatcgcccg gcatccacac gccaccctga
     9481 cgcacaaact ggcgctgtaa ctggttttgc agacgaatgc ccagcacgga aggcggcagc
     9541 gttggcaaaa gcatcagtga acaaggtagt ttttcattca accaacgcca cagtttgtca
     9601 tcagccaggc cgaagcaggc gggcatcagg atcatttcgc aggtattggc gacaggaata
     9661 agcgcatcaa gtaacagcgg ccagttttct tcattatcaa ggaaacgggc gatattcacc
     9721 gcgcggaact cggtggcgtt attgcgcagg acatccagtt ccggcagctc tatttctgcg
     9781 gtttcaacgg caaggccgag ttcacgcaac gaagctgccg caaggtgcgc ctgaaaatcc
     9841 atcagtccac taattcccac tacacatatt ttcttcgcgg gcagcggcca gacggggact
     9901 tctggcgaac ttagccaggt agagcgcaga gtgccgagcg gcgtaatccg ctggtgagcc
     9961 agttctacgc tgccctgcaa ttgcgcaccg ctttcagcga ttaatgcctg cgcctggcaa
    10021 gcgagatcga gcacgcgttg tggcccgaga agagagtaag gatgtgcagg tgcctgctga
    10081 cgcaaagatt ccagtccact gtgaatgtct gtcaccggtt gaccatctgg cagatggctc
    10141 agcaaatcca gcgaaccgga tgagaaatgc agcgcgcttt gaccacgcgt gacaatggca
    10201 cagcgcaggc cgtgtttttg cagttgcagg ccacagagta atccggcgag gccgccgccc
    10261 ataatgacag tatcaaagcg catctttctg ctccttctcc agaccacaca atccctgata
    10321 aacccagcgg gtaaattcgc tttcgcgcag tgcatctccc caggcgatgg gttgcacgcc
    10381 tttccagcgt tcgttaagga aggtggaaag ttgctcgata gattgcgcgg acgtcgtgac
    10441 gttaaaacgt tgcagcagtc cggcagcgcg gcaggcgcag agttcgccct ggcaggtacc
    10501 catccccaca cgggtacgac ggcgtaaatc cagcaggcta ttaacgttta aattttctac
    10561 cgcgtactgc acttcacccg cagttaccgc ttcgcactca cataccaggc tacggtgcag
    10621 acggccttcg ctcagccagg caggcgtacg atcgccatga cgataaaccg cagaaccgcg
    10681 cagcggggca ggcagggaga tgactttacg caaggtaact tcagcgggtt cctgtgaacc
    10741 cggaagtgcc agatcggcgg tcgtacaggg gcgcgtgttg cccagtttgc ggcatacggc
    10801 gtcggtagcc cattccgcca tcagccgata ggtcatcagt ttgccaccgg taatggtgat
    10861 aaatccgtcc agaccatcgc gttcagcatg atcgagcagc acgatgccgc ggctgacgtt
    10921 acgcccgctc ggatcatcat cgctggcgac cagtggacgc acgccagagt aggcccgcaa
    10981 aatgcgcgtt tgcgccatca cgggggccag tttttcccct tcacgcagca ggatatccac
    11041 ttcttctgcc gtcacccgat tatcgtcaat ctcgttgtaa tcaatacgta aagaggtggt
    11101 gccaatcagc gaaatggtat cgccaggcac cagaatatcg gcgtcggaag gtttacggca
    11161 gcggttgatc acatgctggt taatgcggtg atccatgatc agcagcgatc ctttcgccgg
    11221 gaacatgcga atgcgcagat cggcatattc ggcaatgtgt tgcccccaga tcccggcagc
    11281 gttaaccacg acaggcgcat gtagcgactg ggtttcgccg gtgaggtggt tgcgtacatg
    11341 aacgccgcac accgttgcgc cttcacgaat cagtcccgtg acttcatgag cagtaaggat
    11401 aacggcaccg tgttctttgg catccagcat gtttgctgcg gtcagacgaa atggatcaac
    11461 ggtgccatcc ggaactttca ccgcgccaat cagtgccggg ttaacggcag gttcgataat
    11521 gcgcgcttgc tgcgggtcta tagcttctgc gctgatccct gcttcttcgc aggcgcgaat
    11581 aaaagtggcc tggaaggaga ggtcatcttc cggcagggtg ataaacaggc cgttggttgg
    11641 ttcaacgcag tgacgtgcaa tgcgtttcag gatctggttt tcactaatgc attcgcgggc
    11701 cgattccgca tcggttaccg cgtagcgcgc accgctgtgc agcaggccgt ggttacgccc
    11761 ggtggcaccg gttgcgatgt cgtggcgctc aaccaaaatc acgcgcagcc cgcgcagggc
    11821 acagtcgcgg gcaatcccgg ctcccgttgc gccgccgcca atgataatca cgtcacttga
    11881 ttgcgagtcg cgagttttca ttgttatccc tctgaagttc gttttttacc atttagccat
    11941 agtaaaaaca tgaattgttt gatttcgcgc atattcgctc ataattcgaa agtgaaacgt
    12001 gat
//
LOCUS       DAZKFU020000137        11948 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZKFU020000137 DAZKFU020000000
VERSION     DAZKFU020000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.138"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(180..494)
                     /gene="fliE"
                     /locus_tag="ACHOQQ_RS15390"
                     /old_locus_tag="pgaptmp_003078"
     CDS             complement(180..494)
                     /gene="fliE"
                     /locus_tag="ACHOQQ_RS15390"
                     /old_locus_tag="pgaptmp_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU4471293.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            709..2367
                     /gene="fliF"
                     /locus_tag="ACHOQQ_RS15395"
                     /old_locus_tag="pgaptmp_003079"
     CDS             709..2367
                     /gene="fliF"
                     /locus_tag="ACHOQQ_RS15395"
                     /old_locus_tag="pgaptmp_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU4471294.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2360..3355
                     /gene="fliG"
                     /locus_tag="ACHOQQ_RS15400"
                     /old_locus_tag="pgaptmp_003080"
     CDS             2360..3355
                     /gene="fliG"
                     /locus_tag="ACHOQQ_RS15400"
                     /old_locus_tag="pgaptmp_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU4471295.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3348..4034
                     /gene="fliH"
                     /locus_tag="ACHOQQ_RS15405"
                     /old_locus_tag="pgaptmp_003081"
     CDS             3348..4034
                     /gene="fliH"
                     /locus_tag="ACHOQQ_RS15405"
                     /old_locus_tag="pgaptmp_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU4471296.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPIVEPEETIIEETEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKLGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4034..5407
                     /gene="fliI"
                     /locus_tag="ACHOQQ_RS15410"
                     /old_locus_tag="pgaptmp_003082"
     CDS             4034..5407
                     /gene="fliI"
                     /locus_tag="ACHOQQ_RS15410"
                     /old_locus_tag="pgaptmp_003082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU4471297.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISPE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5426..5869
                     /gene="fliJ"
                     /locus_tag="ACHOQQ_RS15415"
                     /old_locus_tag="pgaptmp_003083"
     CDS             5426..5869
                     /gene="fliJ"
                     /locus_tag="ACHOQQ_RS15415"
                     /old_locus_tag="pgaptmp_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU4471298.1"
                     /translation="MTEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAATRKPE"
     gene            5866..6993
                     /gene="fliK"
                     /locus_tag="ACHOQQ_RS15420"
                     /old_locus_tag="pgaptmp_003084"
     CDS             5866..6993
                     /gene="fliK"
                     /locus_tag="ACHOQQ_RS15420"
                     /old_locus_tag="pgaptmp_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU4471299.1"
                     /translation="MIRIAPLITADVDNTTLSGGKASDAAQDFLALLSEALTGEATTD
                     KATPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSTQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLIAPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7098..7562
                     /gene="fliL"
                     /locus_tag="ACHOQQ_RS15425"
                     /old_locus_tag="pgaptmp_003085"
     CDS             7098..7562
                     /gene="fliL"
                     /locus_tag="ACHOQQ_RS15425"
                     /old_locus_tag="pgaptmp_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGU4471300.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7567..8571
                     /gene="fliM"
                     /locus_tag="ACHOQQ_RS15430"
                     /old_locus_tag="pgaptmp_003086"
     CDS             7567..8571
                     /gene="fliM"
                     /locus_tag="ACHOQQ_RS15430"
                     /old_locus_tag="pgaptmp_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGU4471301.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8568..8981
                     /gene="fliN"
                     /locus_tag="ACHOQQ_RS15435"
                     /old_locus_tag="pgaptmp_003087"
     CDS             8568..8981
                     /gene="fliN"
                     /locus_tag="ACHOQQ_RS15435"
                     /old_locus_tag="pgaptmp_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGU4471302.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADTVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            8984..9349
                     /gene="fliO"
                     /locus_tag="ACHOQQ_RS15440"
                     /old_locus_tag="pgaptmp_003088"
     CDS             8984..9349
                     /gene="fliO"
                     /locus_tag="ACHOQQ_RS15440"
                     /old_locus_tag="pgaptmp_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGU4471303.1"
                     /translation="MNNHATVQSSAPISAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9349..10086
                     /gene="fliP"
                     /locus_tag="ACHOQQ_RS15445"
                     /old_locus_tag="pgaptmp_003089"
     CDS             9349..10086
                     /gene="fliP"
                     /locus_tag="ACHOQQ_RS15445"
                     /old_locus_tag="pgaptmp_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU4471304.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10096..10365
                     /gene="fliQ"
                     /locus_tag="ACHOQQ_RS15450"
                     /old_locus_tag="pgaptmp_003090"
     CDS             10096..10365
                     /gene="fliQ"
                     /locus_tag="ACHOQQ_RS15450"
                     /old_locus_tag="pgaptmp_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGU4471305.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10373..11158
                     /gene="fliR"
                     /locus_tag="ACHOQQ_RS15455"
                     /old_locus_tag="pgaptmp_003091"
     CDS             10373..11158
                     /gene="fliR"
                     /locus_tag="ACHOQQ_RS15455"
                     /old_locus_tag="pgaptmp_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU4471306.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            11448..>11948
                     /gene="rcsA"
                     /locus_tag="ACHOQQ_RS15460"
                     /old_locus_tag="pgaptmp_003092"
     CDS             11448..>11948
                     /gene="rcsA"
                     /locus_tag="ACHOQQ_RS15460"
                     /old_locus_tag="pgaptmp_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU4471307.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAK"
BASE COUNT         2879 a         3118 c         3213 g         2738 t
ORIGIN      
        1 gtggtgatgt gtgtcaaatg agacattctg atgatcataa ggtttatcgc gtatacggat
       61 cgtggatggc agaaaacttt cagaatcagg tttccatttt aaaccagaaa ttgagtgagt
      121 ttgccccatc catgccccac gcggtgagat cggatgtaat taacaacagg ttacaaaacc
      181 tacacctgca tgctcatcac ttcctgatac gccgccacca gcttattacg cacctgaatc
      241 cccatttgca tagaaactga ggctttttgc atatcggtca tcacatcgtt taacgccacg
      301 ccgggttcac cgagagtgaa tttttctgcc tgcgtgcggg cggctgtttg tgtatcgctt
      361 atgcgatcga gcgcggcgtg cagctgcccg gcaaaactaa tggtcggttg cggcagtgat
      421 tcctgcgcac gcgcactcat cgccgtagcc tgtaactggc tgataacccc ttcaatcccc
      481 tgtatcgctg acattttcat ctcctggata atttctggta gcaaagccta ccagtaagtc
      541 aataagacaa aggcgctaaa tagcaacaaa aaaacgggtt tattggcgga tagaaaaaaa
      601 cgaaagcaca aataatggga gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
      661 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
      721 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
      781 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
      841 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
      901 agccaactga cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgaa
      961 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgccaaaa
     1021 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     1081 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     1141 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     1201 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     1261 gaagggcaaa ttagcgccat tgtgcatctg gtttccagcg ccgttgctgg tctgccgccg
     1321 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     1381 cgcgatctta atgacgctca attgaaatat gccagcgatg tcgaaggacg tattcagcgg
     1441 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggttacg
     1501 gcgcagctgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     1561 gaatctcagg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     1621 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     1681 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     1741 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     1801 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt tgtggtgaat
     1861 tacaaaacct tgccagacgg taaaccgttg cctctcagca acgaacagat gaagcaaatt
     1921 gaagatctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     1981 gttaactcgc cgttcaatag tagtgaggaa agcggcggag aactgccgtt ctggcaacag
     2041 caagcgttta tcgatcagtt actggctgcc ggtcgctggt tgctggtact gctggtggcg
     2101 tggctgctgt ggcgtaaagc ggtacgcccg cagttaacac gtcgcgctga ggcgatgaaa
     2161 gctgtacagc aacaggcgca ggcccgcgag gaagtggaag atgcggtgga agtccgcctg
     2221 agcaaagacg aacaactcca acaacggcgc gctaaccaac gtctgggggc agaagtcatg
     2281 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     2341 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     2401 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaggt
     2461 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccga
     2521 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     2581 cgattatctg cgttcggtat tggtcaaagc tctgggtgaa gaacgtgccg ccagcctgct
     2641 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     2701 gccacagagc gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     2761 ggtgcatctg aagcgcgccc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     2821 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcaga
     2881 gctgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     2941 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     3001 tattaccgcc gtgcgtgaat tcgacggcga actggcgcag aaaatcatcg acgagatgtt
     3061 cctgttcgag aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     3121 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     3181 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     3241 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     3301 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     3361 tgccctggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccaa
     3421 tagttgagcc ggaagaaacc atcattgaag agaccgaacc cagccttgag cagcaactgg
     3481 cgcaactgca aatgcaggcc cacgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     3541 agcaaggtca taagctgggc tatcaggaag gactggctca ggggctggag caaggtctgg
     3601 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     3661 ttcaaactac ccttgatgca cttgatagtg tgatagcgtc gcgcctgatg cagatggcgc
     3721 tagaggcggc acgtcaggtc atcggtcaga cgccaacagt ggataactcg gcactgatca
     3781 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     3841 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgctacctta agtttgcatg
     3901 gctggcgctt gcggggcgat cccaccctcc atcctggcgg ctgtaaagtc tccgccgatg
     3961 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     4021 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     4081 agccaaaatg gcgcagttgc ctgcggtacg tcgctacggg cgattaaccc gcgctaccgg
     4141 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgagcg
     4201 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     4261 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gtgtttatgc
     4321 caaaaacatt tcgcctgaag ggctacaaag cggcaaacag ttgccgctcg gtccggcgtt
     4381 attaggtcgc gttctggacg gcagcggtaa accgctcgat ggcctgccct cccccgatac
     4441 gacggaaacc ggtgcgctga ttaccccacc atttaaccca ttgcaacgta caccgattga
     4501 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgcgggca
     4561 gcgtatgggt ctgtttgctg gttctggtgt cggtaaaagt gtgctgctgg ggatgatggc
     4621 ccgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     4681 aaaagatttt attgagaata tcctcggtgc cgaagggcgt gcacgatcag tggtgattgc
     4741 cgctccggcg gatgtgtctc cgctcctgcg aatgcaaggt gccgcctatg ccacgcgcat
     4801 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     4861 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     4921 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     4981 tagcggcggc ggctcgatta ccgcgtttta taccgtgctc accgaaggcg atgaccagca
     5041 ggaccccatt gccgactccg cgcgggccat cctcgacggt catattgtgc tgtctcgccg
     5101 actggcggaa gccgggcact atccggcgat tgatattgaa gcgtcgatca gccgcgcaat
     5161 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     5221 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     5281 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttgc aacaaggcat
     5341 ttttgaacgc gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     5401 gtcataaccc aggagataac ggcagatgac agaacatggc gcgctggcga ccctgaaaga
     5461 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcggatg
     5521 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     5581 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     5641 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     5701 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     5761 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     5821 aaagatggat gagttcgccc agcgcgccgc cacgaggaaa cctgaatgat tcgcatagcg
     5881 cccttgatta ccgctgacgt tgataacacc acattgtctg gcggcaaagc cagcgatgct
     5941 gcacaagatt ttctcgcgtt gttgagcgaa gcattaacag gcgaggcaac aaccgacaaa
     6001 gcgactcccc agttgctggt ggcaacagat aagcccacga caaaaggcga gccgctggtc
     6061 agcgatattg tttccgacgc gcaacaagcg gatttactga tccccgtgga tgaaacactg
     6121 cctgtcatca acgacgaaca atccacatca acaccattaa ccaccgctca aacgatgacg
     6181 ttggctgcgg tggctggcaa caatacggca aaagacgaaa aagcggatga tctgaatgaa
     6241 gacgtcaccg caagcctgag tgcccttttt gcgatgttgc cgggttttga caatacgccc
     6301 aaagtgactg atgcgccgtc aaccgtgtta ccgacagaga aaccaacgct cttcacaaaa
     6361 ctgacttcta cgcaactcac aacagcacag cctgatgacg cccccggcac gccagctcag
     6421 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     6481 ccggtgaccg ctgccgccag cccgctaatc gctccacacc agacacagcc actgcccacc
     6541 gtcgccgcgc ctgttttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     6601 cagcatattt cgctgttcac ccgccagggg caacaaagtg cagagttgcg tctgcacccg
     6661 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     6721 atggtttcac cccatcagca tgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     6781 acgcagctgg ccgaaagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     6841 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     6901 cctctggcgg gggaagacga cgatacgctt ccggttcccg tctctttaca agggcgtgta
     6961 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     7021 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     7081 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     7141 ggatcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     7201 ggcattcgca tcaggttgcc gctgacgaca aagcgcagca acgcgtcgtg ccctcaccgg
     7261 tcttctatgc gctggatacc tttacggtca atctgggcga tgcggatcgc gtgctttata
     7321 tcggcataac cctgcgcctg aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     7381 cggaagtccg tagtcgcttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     7441 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
     7501 ttgccgggca accgaaacag gatgtcaccg acgtgctgta taccgctttt attctgcgat
     7561 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
     7621 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
     7681 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
     7741 gagcgctttg ctcgccattt tcgtatgggg ctgttcaacc tgctgcgccg tagcccggat
     7801 attaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
     7861 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttc
     7921 tcgccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcgatgg acgcttcccg
     7981 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgttg
     8041 aaactggcgc ttgaaggcta tagcgacgca tggaaggcga ttaatccgct ggaagttgag
     8101 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
     8161 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgc
     8221 ctgcctttca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
     8281 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
     8341 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
     8401 aaacccggtg acgtcctgcc gatagaaaaa cccgatcgca tcatcgccca tgttgacggc
     8461 gtcccggtgc tgaccagtca gtacggcacc ctcaacggtc agtatgcgtt acggatagaa
     8521 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
     8581 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
     8641 aacaaaaatc aaccagtagc aaaagcgctg ccgacacggt gttccagcaa tttggcggtg
     8701 gtgatgtcag cggaacgttg caggatatcg acctgattat ggatattccg gtcaagctga
     8761 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
     8821 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
     8881 taatcgccca gggcgaagtt gtcgtagtcg ctgataaata tggcgtgcgg atcaccgata
     8941 ttattacccc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
     9001 tgtgcaatct tccgcgccga tttctgctgc gccactgctg caggtgagcg gcgcactcat
     9061 cgccattatt gccctgatcc tcgctgctgc ctggctagta aaacggctgg gatttgcccc
     9121 taaacgaact ggcgttaacg gtctgaaaat tagtgccagt gcttcgctgg gcgcgcgtga
     9181 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
     9241 aatcaatctg ctgcataaac ttcccccttc tgcaccaacg gaagagatac cgcagaccga
     9301 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagcggg agatcctgat gcgtcgttta
     9361 ttgtctgtcg cacctgtcct tctctggctg attacgcccc tcgccttcgc gcaactgccg
     9421 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcaaacg
     9481 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
     9541 acccgcatca ttattgtttt tggtttattg cgtaacgcgc tgggaacacc ctccgcgcca
     9601 cctaaccagg tattgctggg gctggcactg tttttgacct tttttattat gtcaccggtg
     9661 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
     9721 gaggcgctgg aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca gacccgtgag
     9781 gcggatttag ggttgtttgc cagactggcg aataccggcc cgttgcaggg acctgaagcc
     9841 gtgccgatgc gcattttgct cccggcctac gtgaccagcg agttgaaaac cgcatttcag
     9901 ataggcttca cgattttcat cccttttttg attatcgacc tggtgatagc cagcgtgttg
     9961 atggcattgg ggatgatgat ggttcccccc gccaccattg ctctgccctt taaactgatg
    10021 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    10081 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    10141 gtcgcgctgg cactggctgc cccgctattg ttggtagcgt tggtcacggg ccttatcatc
    10201 agtattttgc aggctgccac gcagattaac gaaatgacgc tgtcgtttat tccgaaaatc
    10261 atcgccgtat ttatcgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    10321 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtagccgta ctatgttgca
    10381 ggtgacaagc gaacaatggc tatcctggtt aagcctgtac ttctggccgt tactgcgcgt
    10441 gctggcgctc atttctaccg caccaattct gagcgaacgc agcgtgccga aacgggtaaa
    10501 actggggctg gcaatgatga tcacgttcgc cattgcccca tcattacctg ccaacgatgt
    10561 tcctgttttt tcgttctttg ctctgtggct ggccgtgcag cagatcctga tcggcattgc
    10621 gcttggtttt accatgcaat ttgcctttgc cgctgtgcga accgctggcg aaattatcgg
    10681 tctgcaaatg gggctgtcat ttgcgacgtt tgtcgatccg gccagccatc tcaatatgcc
    10741 cgttttagcg cgtatcatgg atatgctggc gttactgctg ttcctgacat ttaacggtca
    10801 tttatggttg atttcactgc tggtcgatac ctttcacacc ctgccgattg gtggcgaacc
    10861 gttgaacagc aatgcgtttc tggcactcac caaagcaggg agtttgattt tccttaacgg
    10921 gctgatgctg gcgttaccgc tcattactct gctgctgaca ctgaatctgg cattaggttt
    10981 acttaatcgt atggccccgc aattatccat ttttgttatt ggatttccat taactctgac
    11041 tgtcggtatc tctttaatgg cggcattaat gccgttaatt gcgccttttt gcgaacattt
    11101 attcagtgag atgtttaatt tgctggctga tattattagt gaattgccat taatataatt
    11161 ccgtaacgtt tatcatgtta tcctaaggat tatccgaaaa ataataccta cgaacatctt
    11221 ccaggatact cctgcggcga aatatttgtt ttaagctcac tcacatatcg caacatttac
    11281 tttactttaa gacaattcca ggcaaattat acaacacttt acgggatagt aagtccgcct
    11341 gaaaaatcgc gagagtggcg cattaggtga cccatgttgt tccgtttagt catgatgaaa
    11401 tattcaggta aggggaatta tcgttacgca ttgagtgagg gtatgccatg tcaacgatta
    11461 ttatggattt atgtagttac acccgactag gtttaaccgg gtatctgttg agtagagggg
    11521 ttaaaaaaag agaaatcaac gacattgaaa ccgttgatga ccttgccata gcttgtgatt
    11581 cacagcgccc ttcagtggtg tttattaatg aggactgttt catccacgat gcttctaaca
    11641 gtcagcgtat caagctcatc attaatcaac atcccaatac gttatttatc gtttttatgg
    11701 caattgccaa tgttcatttt gatgaatatc tattggtcag aaaaaattta ttgatcagtt
    11761 ctaaatcgat taaaccggaa tctctcgacg atatccttgg cgatattctg aaaaaagaga
    11821 caacgataac ctcgttttta aatatgccga cgttatcatt gagccgaacc gaatcgagta
    11881 tgttgcgaat gtggatggca ggtcagggaa ccattcaaat ctccgaccaa atgaatatca
    11941 aagccaag
//
LOCUS       DAZKFU020000138        11935 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZKFU020000138 DAZKFU020000000
VERSION     DAZKFU020000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.139"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(28..369)
                     /locus_tag="ACHOQQ_RS15465"
                     /old_locus_tag="pgaptmp_003093"
     CDS             complement(28..369)
                     /locus_tag="ACHOQQ_RS15465"
                     /old_locus_tag="pgaptmp_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4471308.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVR"
     gene            complement(362..3604)
                     /locus_tag="ACHOQQ_RS15470"
                     /old_locus_tag="pgaptmp_003094"
     CDS             complement(362..3604)
                     /locus_tag="ACHOQQ_RS15470"
                     /old_locus_tag="pgaptmp_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4471309.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAVTQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEERTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLSNP
                     GWTTGNGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLD
                     DKQQGALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSETEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREEALKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(3668..4081)
                     /locus_tag="ACHOQQ_RS15475"
                     /old_locus_tag="pgaptmp_003095"
     CDS             complement(3668..4081)
                     /locus_tag="ACHOQQ_RS15475"
                     /old_locus_tag="pgaptmp_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471310.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLEKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            complement(4145..4354)
                     /locus_tag="ACHOQQ_RS15480"
                     /old_locus_tag="pgaptmp_003096"
     CDS             complement(4145..4354)
                     /locus_tag="ACHOQQ_RS15480"
                     /old_locus_tag="pgaptmp_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGU4471311.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEADPLAALEDALLFASEN"
     gene            complement(4450..4824)
                     /locus_tag="ACHOQQ_RS15485"
                     /old_locus_tag="pgaptmp_003097"
     CDS             complement(4450..4824)
                     /locus_tag="ACHOQQ_RS15485"
                     /old_locus_tag="pgaptmp_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4471312.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(4839..5555)
                     /locus_tag="ACHOQQ_RS15490"
                     /old_locus_tag="pgaptmp_003098"
     CDS             complement(4839..5555)
                     /locus_tag="ACHOQQ_RS15490"
                     /old_locus_tag="pgaptmp_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU4471313.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(5621..5965)
                     /locus_tag="ACHOQQ_RS15495"
                     /old_locus_tag="pgaptmp_003099"
     CDS             complement(5621..5965)
                     /locus_tag="ACHOQQ_RS15495"
                     /old_locus_tag="pgaptmp_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HGU4471314.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(5962..6408)
                     /locus_tag="ACHOQQ_RS15500"
                     /old_locus_tag="pgaptmp_003100"
     CDS             complement(5962..6408)
                     /locus_tag="ACHOQQ_RS15500"
                     /old_locus_tag="pgaptmp_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGU4471315.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGIESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(6405..6755)
                     /locus_tag="ACHOQQ_RS15505"
                     /old_locus_tag="pgaptmp_003101"
     CDS             complement(6405..6755)
                     /locus_tag="ACHOQQ_RS15505"
                     /old_locus_tag="pgaptmp_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4471316.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(6765..7091)
                     /locus_tag="ACHOQQ_RS15510"
                     /old_locus_tag="pgaptmp_003102"
     CDS             complement(6765..7091)
                     /locus_tag="ACHOQQ_RS15510"
                     /old_locus_tag="pgaptmp_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4471317.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(7088..9510)
                     /locus_tag="ACHOQQ_RS15515"
                     /old_locus_tag="pgaptmp_003103"
                     /pseudo
     CDS             complement(7088..9510)
                     /locus_tag="ACHOQQ_RS15515"
                     /old_locus_tag="pgaptmp_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(9456..9677)
                     /locus_tag="ACHOQQ_RS15520"
                     /old_locus_tag="pgaptmp_003104"
     CDS             complement(9456..9677)
                     /locus_tag="ACHOQQ_RS15520"
                     /old_locus_tag="pgaptmp_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471318.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVQRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(9722..11659)
                     /locus_tag="ACHOQQ_RS15525"
                     /old_locus_tag="pgaptmp_003105"
     CDS             complement(9722..11659)
                     /locus_tag="ACHOQQ_RS15525"
                     /old_locus_tag="pgaptmp_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4471319.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGVHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(11723..>11935)
                     /locus_tag="ACHOQQ_RS15530"
                     /old_locus_tag="pgaptmp_003106"
     CDS             complement(11723..>11935)
                     /locus_tag="ACHOQQ_RS15530"
                     /old_locus_tag="pgaptmp_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4471320.1"
                     /translation="AEGVLVHGGQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPL
                     MALFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT         2489 a         3887 c         2861 g         2698 t
ORIGIN      
        1 aataaagagt gatttatagg ataattctca tctgactacc tgctctatcg tgcaactgaa
       61 atcactgtac cgggcgttat ccgtaatact ccactcccgg cacaccaccc tgaccgtccg
      121 gttatgtttc gggggcttcc acaaaaaagc ccggtaacca ccatgccatg acagaaacgc
      181 ggacagtgcc tcccgctcag catccgttgt cacccgaaaa accacctgaa aggtcttcag
      241 ttgcgcattc agtcctgccg gacgacgttg ctgataaccg tcgccaaact tcaccgtcac
      301 caccgacggt ttctccgtca cctgcatccc ttcccgggga caccagtgaa gggtcttaat
      361 ctcatccact cagcattcct ccgtcacgac gcatggataa catcaccgcc tgtacccgct
      421 ggtcaatcag ttgcacaagg gtgcccgccg cttccggccc tatctgcccg ttagcgccgt
      481 cattctgaat ggcaatgtgg tacaccgggg aatacaccag acccgcactg ccgttcatac
      541 tgcccaccgc acgcacgccg agtgaaccat ccgctgcccg ggtcaggggc atgatggctt
      601 caggtccggc ttcccccatc agccctgccc cttttgcaaa ggcaaagtac gtgggcgtgt
      661 ccacaatact gttactgtat gcactcaggt ttgccgaggt ataaacgccg ccttttgcat
      721 tggccaccgc gccacccagc cagttgccta tactgccgaa aaatcctcct gcaccggaca
      781 tactgtttgc ggccatctta atgccgttga caatggccgc attcataaga acttttgata
      841 tttcctgcag gattgatgca gcccagctgc gccattccac tttatttccg ttcagcatct
      901 ccgtgatgtt attcaccatc cctgagatac cgtctgtcgc aagctgtgct gcctgtgaag
      961 cgtaatctga tgcgctgtcc acccagttac ttagcccttc ctgcagccct ttctgccagt
     1021 cagcacgctg cgcatccgat tcggcataaa aagcctcctg gtctttaagg cgttcactca
     1081 gatactgcgc attctgcgcc agcgcctgtc tgtaaaaatc ctcactgata tcccctgtct
     1141 gatactgaga ctgcaggtcc gcatccttct ggcggtagcc gtcacgaatc tgctgcagct
     1201 cccgcatccg ttcacggacc cgctcaccct gcccgtaccc cagcagctca gcgtcatttg
     1261 atgccctttc gtctgcatta tcatttttca gagcctcctc ccgggcccgt aactgctccc
     1321 ggattttctt ctggtcaatc agggcagcat tgcgcagcaa ctcctgcttc tgtatctccg
     1381 tcagggtttt cagctctccc tgcgaggtct ggtacttcag cttcgccagt tctgtattct
     1441 tccctgccag tgccagttgt tccttctgct gcttcagcag acgggaaaaa ctgtcttccg
     1501 ctttttccgt ctcagatttt ccggcccggg atttgggttt attcgcctca ttattgcgcc
     1561 aggcttccat cgaattacgg atgtaacgct gcctcgcctc ctgatacgta tcccccacca
     1621 gaccaaggtc atccgccgca taccccagcc gggcacgctc tttttcttcc cctttcagcc
     1681 gggacagggc cagttgacgc tctgtgttat tcagggcgcc ctgctgttta tcatccagag
     1741 tggcctgtgg cagtcgcagc ggtacactcg ccagtccctg acgctgttgc agcagttcat
     1801 tacccagccc cagcaggcgg ttgaattccg tatgctgacc gttcatgatc agcagtgact
     1861 gatacgcttt attctgctct gccgcctgct gacgaattaa cacaacacgg cgctcttcca
     1921 gtccggcaag cacatcctga atggactgcg ctttttcctg catctgtgcc agacgggact
     1981 gctcaacagc aagctgctct gttgcctgag caagcccttc cgttacagtt ttcaccgatg
     2041 tcaggtggtt tatcatgaag ccgttaccgg tcgtccagcc cggattagac agcacatact
     2101 gatacccggc gattttttcc tgcagggatt tcacccggct ggcctgctca tcaatcagcc
     2161 ggttctgctc tgtcagcgcc tcccgtgttc gctcttcatt atctgaggct tcaggcagag
     2221 acattgatgg cgttttatgc gcgatttcat caatcgtcag tgcatactgg cgcgcagact
     2281 ccctggcctg ttcctgattc tggtacagcg tgtaccatgc ggcagctccc agcatcacca
     2341 gtccgggtac gcctccaacc agtcccagcg caccgctcat cagacgtgag cccaccgccg
     2401 ttgtactgtt cagcgcattc tgggcggcgg ttctggcagc aatatttctg ttcaggcgtt
     2461 cctgtgtgac cgccagacgg gcctctgccg caatctgcat ctcagtcccg cgggctgccg
     2521 ccacagcctg ctgagcccgg tacacggctg cccttgcccg cgccgtggca atctgcgttc
     2581 cccttaactg ggcctcagcc agtgccactt cattacgtgc agcggtcaca agtcctgccg
     2641 tggcagacat cgctccggaa gccagattac caaagtaccg ggccaccccg acagcaacca
     2701 gcacacccgc agctgttgcc acggtatcaa tatttccggc cacaccgttc agtgcgccag
     2761 agagcgtttt cgtcgctccg ctggcctcat tcgcgccgcc cacccaggcc ataaaggcgt
     2821 tttccacctt cgtgatcccg tcagacaccg tttccggcat ggctgcatat tcatcacgca
     2881 atatccccag ctggctgatt aacgcgggaa cgactttatc cgccgtcagt tgaccatcgt
     2941 ccgccatcgc cttcagatcc ttacgggcca cgcccatgcc tgcagccagt gcgcgaatga
     3001 tccggtcccc actttcattg accgaattaa attcctcgcc gcgcaacacc ccctgtgcca
     3061 gcgcctggct gaactgggtg atcaccgaac ccgcctctgc cgcactggca ccggaaattt
     3121 tcagccctgt cgaaatggcc tccgtcacct tcagcacatc atcagcactg taaccatatt
     3181 cacgcatcga ggcagccgaa cgggcaaaca gggccgcatt atccgaaaat gcggtgcctg
     3241 tccgctggct gatatccatc agcactttct gtgatgacga aaattcatcc gatgactgcg
     3301 acgcctgttt cagacgggca ttcacggagc tccattcatc cgccagcgaa atcaggtgtc
     3361 cggtggcaaa ggcaccagca aacgcaccgg tcattccgac agccgaagcg cggatttccg
     3421 tcaactggct gttcagctca gccagagccc ggcgctgctc cctggctgcc gcagcagcct
     3481 gacgtccgcc attctgcagg gtccggtaat attcactgcc catacgggac gcccgctgga
     3541 tctccgactg gaatgactgt gaatttgccg aaattttgat aatcagttca cgtaacgtcg
     3601 ccattcacct ttctccgggc gaaaaaaaac ctgccgcagc aggttttatt catcagcaga
     3661 ttattgatta agttttgggt accagcccgg gattcagact ttccggactc agtgtataca
     3721 taccgccatt gaatgggtcc acaagcaacc agccaacaat accaccaaag acaaagttac
     3781 caccaatgta ccatccatta gccgatgcct tgatcggcag cgttacgggc tgataattct
     3841 ctttactgaa tgtgatctga taactctttt tcccgaagta actgccgtca gatttttcaa
     3901 gtgttacatt ctgcggtgtt ttgcccgaag aaattgtttt ccctgactca tctttaataa
     3961 taaaatccgc accagacgga ttactgttta cctgtacagt ctgcgtttta tctccgacaa
     4021 tggttgcaca ccccgtcaga aaaaagctga cagagaaaat caacagcatg aatttcttca
     4081 taaaatggct ccgttcagtt aattttcccc gaaaatactg taataaagat ctgcaaagtt
     4141 aaaattaatt ttccgaagca aataacagag catcctccag agcggccaaa ggatctgcct
     4201 ccggcttatc ctcatcctcg ccccagcaga gcatggcgtc cttcagtgca acattcatcc
     4261 cctgtgcccc gaaaaccgct ttcacgatct gtgcattacg gatatccccg cgctcatcac
     4321 ccagcgggga taccctgtcg aactccatcc acatcatcgc ctcgctcgca ctcaggctgt
     4381 ggcgcagttc ggataaggtg cgccccagac ggagcgcaag tcgcatcaga aagcgaattt
     4441 ccgggcgggc tacttttttc tggccgactc tgcatcagcg atcagttcca gtgcctgacg
     4501 cagcaagcgg gcatgtaccg gaccatagac ggccagcacc tgctcacggt cgtccggagt
     4561 gaacacccgc tgcaggtccg tatcacacag gacatcgcag aacagcgtca catccgcttc
     4621 caggttacgg cgggttttcg ccaccaccga cagggtatcg tcatcctctc catcaccatt
     4681 gagcacgtcc tgccacagat accaggcctc tgccgaaggc tcccgcagca ccacgctgac
     4741 attaccccat tccggcacct tcaccgtttt atgacgaaac cctgacagtc tggccagcgc
     4801 cagcgttttc agatcctttt tcatgatgac ccatcccctt atccggcggc tgcgctcact
     4861 gtcactgtac attcaacaga cgtcacactc tgtgctttct ctgccgaatc agtcaccacg
     4921 caggtatatt tccccgcatc agcggactgc gcacctggct tactgaaggt gtctgtcgtc
     4981 tgcccgtcaa ccggctgacc atccttcttc caggcgtatt tatacggcgg cgttcccccg
     5041 ttggcactga ctgacattgt cagcagcgca ccggtattca cggtaagtgt cttatccaga
     5101 tttttcacaa acgccagcgg taccacaaag gacaccggtt tgcctttcag acgcagtgag
     5161 aacgttgcag ccaccacgcc gttggtaccg gatgaccagg tgtgctggcg cacttccgcc
     5221 aggaatttaa agcccttacc ggacggaaac tgcaccttaa acgcatacaa cgcgtcattg
     5281 tcataagcat cacgcagggc gttctgggcc tgattcagat aaaaattacc cgacatggaa
     5341 atctcggacg acgcccccag accattgata ttctcctgct ctgtggagca gagcgtggtc
     5401 acatcaatat cctgtttctg accggcggtg aactggactt ccttgatggt gcagtccagg
     5461 cgcagatatt ccgccttatc catagtttca gcagtcgccg gggcagatga aatcatcacc
     5521 tgcgtcagct gtgagcgttc atacaaagca gacattctgc ctcctgataa taaaaaaccc
     5581 gcacgcggcg gggtatgggt tttgtagaaa aaagaaaaag tcacaccgtg acctgaaact
     5641 ccagggttgc acggtaacag cggttttccg gaatatagtc ctgcatttca ctgacggatc
     5701 ccggggccag cagcattatg gcttcacggg cgtcctgacg tatctgacgc gcctgcgtca
     5761 cagtcccggc ataaacgtct atctgcaccg ccactgagga ctccgcctgc ccgcccatca
     5821 cgtccgcaga caccgatgaa atcaggctga aaaccaccca cggaagcgcc accgacggcc
     5881 tgccatccag cagggggacc acatacgggt acacctgccc gccggcaaga tgcgccagat
     5941 gaggatacaa atccgcctcc gtcatcgtct cagtacctca tcaatggccc ggttcatccg
     6001 cgcaatcgcc acctgcgctg cctgttcact gcgcacatca aacgccgggc gcacaaacgg
     6061 gtgcggtggc atattcacgg tccccatttc cacaaaccgc cagtagaaag cattccgcgg
     6121 gttatctgcc ttcatggtgt tatcgctgtt accggtgtcc ggattaacac cacggatatg
     6181 gacaccggat tctatcccgc catcgcggga gcgccgggaa aggaccacca cattgcggcg
     6241 cagttttccc ctgcgcaccg gtgcccgtga caccacttct tctttcagca cattcgcacc
     6301 cgcacgggtt gcctcacgca gcacccggtt attttccgca ccactcagaa gctgcaaatc
     6361 gcggctgatg tcctccagcc ccgaaaaatc cagcagggtt tcgatcattt ttcccctccc
     6421 agccgacaga gaatttccag acgcccgccg gtcgcatccg gcacgggcag cccgacaacg
     6481 ttcaggatcc ggtcacgcca tggaccactc agcacatgaa gtcgtgacgc tgccgtgatt
     6541 tcccgaccgg actgaccgcg cacccagatg cggatttccg cctgcgccat ttccgcaccg
     6601 gactgcatcc gctcccggct gctcctgcca cggatatccg catgaatttt cccgcatgac
     6661 acccattctt ccgtcatttc tccggcagca ttacgggtta acaccgggtt cagaacactt
     6721 atcatctgtg tcagacgacc tgcagatatt gccattcccc ctcctcataa caccgtcgga
     6781 caacgcaaat cgtaaatcag cacggaaaca gaaaacggca gctccccctg aagcagttct
     6841 tcccgctccg caagatccgg attccggtac agcatcccgg tcagtcgcat ggcagccccc
     6901 ttcatccggg ttaatgcctc gcccgggatc agttcaccgt cctcacgaat caccttatcc
     6961 cggctgccct gaatgtaggc cagcagcacg gcggtagcct gacgaacctt gtccatcagc
     7021 atgtcatcat ccgcgtcatg gtcgacacgc agatgtgcct tgatctcttc cagtgtcagt
     7081 aatgccgtca ttttccacct cctgcatccc gcccacgttt tgcagccagg gtccaggctg
     7141 atgaatgagc ttctccgggt ttatcaccgg tcatactgtt gcagtgccac agcgagcccc
     7201 cccatgtcac cgtatcgccg gggtggtagg tttcaccggc tctgaacaca ccgcggtaga
     7261 gcatcaccgg cagggaaaat gttttttccg tacgctggcc actgctctgc cggaccacca
     7321 cagagaacaa ccgttcaccc gtcatgctga cgtcaatatc cgccaccccg tcaaccaggc
     7381 attcccatcc ccgcatcccg tgcgtttttt catacgcccg ccagagtccg ccctggtgtg
     7441 tggcatacgt gccccgggga aaggattttt gatcgtcaat ggcagggagt atttccagtg
     7501 ccgtggcatc acgcccgtcc tgcggagccg gcagggcact caccgcatcc agaaccgcct
     7561 tctgcagaac atcgggatca tagtcacgac catcacgcgg aacaggaata tggcttaccg
     7621 cctccttcac catctgttca agcatcggac gcacatcatc cggggtgata cttttaccgt
     7681 ccgccggctg cggaatattt gcgaccgcat cattcaccgc cttctgcaga acatcgggat
     7741 catagtcacg accatctcgc ggaacaggga tatggcttac cgcctccttc accatctgtt
     7801 caagcatcgg acgcacatca tccggggtga gacttttacc gtccgccggc tgcggaatat
     7861 ttgcgaccgc gtcattcacc gccttctgca gtacttccgg atcgtagtca cgaccgtcac
     7921 gcggaacagg gatatggctt accgcctcct tcaccatctt ctcaagcatc ggacgcacat
     7981 catcgcccgt cacgcacttc tgtaatacca ctgacaggga cgccagtttt tcttcaaacg
     8041 tttgtgcctg cgcggctatt ttcccctcaa atgtgcgctg taaatctgcc agcactgcgg
     8101 aaaattcttc acccagcgca cggataatgg acagttcacg ttcattcatt ttttcagaat
     8161 ccccctgaac atcgccttca ccgcatcatg ctctgtttca gtgattgcct tattaccgtc
     8221 agaggcagcg accgcctgag gcacggaagc tgttttaccg gacgacgcga acggatcttc
     8281 acgggcatca cggcgggaca acgcttccag actgtagttc tgctgctgaa gatacagtgc
     8341 atcaccgccg gccaggggcg gcaggttctc ccgtttacgg gcctcattgg gcgtgagaag
     8401 cgtatttttc accgcatccc ccagcgtttt catgcgccgc tcactgtcca ttctcagcag
     8461 cgtggtgaca tcaaattccg tactctcgtt ttcccccgtt tccagcgcct catccagtaa
     8521 cagctcaatg gactcaatca gtgtctgcag gcactgggaa taatactgct gctccagcgc
     8581 ctccacgttg tcactggaag gcggttgtcc cacgccaatc ttgtaggccg ggacacggaa
     8641 caccgaacag acaatttcag cggtcatctt cagttgttcc accgtctgtg catccaccgg
     8701 tgaaaacgtc gtggggttgt attttgcccc gttgctcaga atggccgttt tccccgcatt
     8761 ttcgcctgta tacccgctgt cccagttgct cttcagtttt ttcgcatttt cttccgtaat
     8821 actgccgggg atctcaatca ccccggacgg cctgccgcca tttctgaaaa aagacgtcga
     8881 attttcctga atatgatgcc cctgcgtggc cgccagcccg gcggcataca ccggcggcaa
     8941 ccctataagc ggatgaaaaa aacagttaaa ccggtcgtgg atcacttccc gggcaggcac
     9001 cgtcaccgcc tcagggatcc cgcagttccg gtccggcgtg atgcggtaga acacctcgcc
     9061 gtcatccgcc accagaggtt caacccggtt ccagtccaga atacgcagtt ctttgatctg
     9121 cccccgggca ttacggattt tcagcaccac cgtattgccg tgacgcagtt tggcgttcag
     9181 ccacagttca aaaaactgga tgcggttctg ctgggcgttg ggacgacgac agaggcgggc
     9241 aatatccccc cggcgcgttt ccctgcgtat cccctgcgca tccgtctgca tcaggcgcag
     9301 ccgcattttg gcgatatcct gggatatcag cgaaatacat gcaaacaccg catgaaagga
     9361 gaggacggct tcaggatcgg ctttcacgcc ctgctgccag gcaccggaaa agggctcagc
     9421 caccgcctga aacaggctgg tccagcccgc ctctcttacg tcacgtcctg atttctggtt
     9481 ttttcgggtt cgccgtaaaa ggttccacat tcgccatgct ccgcatcacg tttctttttc
     9541 tgacctgccg gacgttgcac cgtgatgtac tccgcctttc ccaggcgaac cagcacctcc
     9601 gcacacggct gtgccacatc acggatatcc ccggcccggg catcatgcgt gccctgcaga
     9661 tatcggatct ttgccataac ctgttacggg aggcgcacgc ctcccgtcct ccttatcaga
     9721 ctcagccgcc ggacgcactg ccgtagttca ctccggtgat caccgccacc gccgcggtac
     9781 ggcgacgacg ccagttgatc cagcgctccg cacggatggc cacgctgcct gtctggaaca
     9841 tggaaaccag ctccaccggc gacggcgtgg tactgtcgcc ggtcggctca gactgcattt
     9901 ccagtgatgc ctcgcgggac atatccactg ccacgccgcc gtcatccgcc agataaatat
     9961 ccggggcatt caccagcacc agctggtcac ccacgtactg ggagacaatc accggcagcc
    10021 cctggaagga gccacccagc agggtcatgt ccgggtattc tttctgaccc agcgcatttt
    10081 tacgcatgga cagtgccagg gcattggtgc tggacatcag ccagaccgca ccggtgggtt
    10141 gcaggtttgc tgccacaaac tgtccaaacg cagcctctgc atccgcatcc gggttaccgc
    10201 ttgatgccgt gcccttcaca tcatgggtga tggacgccgg ggagacatct gccaccgccg
    10261 cttttttcgg gtccacaaag tctgtatcca gacgcgccac caccgcttcc gccagcgcat
    10321 tacggaccag tgcatcagca gccggactgg aaaaacggat caattcttcc gtcagtaccg
    10381 caatggccga caccttcgca tgactgaagg tgatggattc aaaatcaaac ttcgtcaggg
    10441 gttttgcctt accctcaccc acccagccgg cagcaccgcc ggacacctgg gcgtgcacac
    10501 ggatattgaa cggcacctga cgaagtgcag ggatcccgcc ctgaccaaat cgcccgataa
    10561 tggtctgagg acgcaggtaa tcaataaagt cctgtgcgta ttcctgatat tcagacaggc
    10621 tgcctgccca ctgcggatcc gtggtggtcc ccgcgcccac tgccgatttc aggacatgat
    10681 gcagacgact gtcatccgga tactgacgac gggccacttc cagggcttca gagcggacac
    10741 ctttagccgc agccagcgat ttggcaaaac gtgcgaaacc aatcccctta tccagtttct
    10801 gctccacacg gatcaccggc gcagaagcca ccgcggccac attcccgtta ccggcctgtt
    10861 tcaccggctg cgccgtggcg gccttaccgg cttccagttc acgcaggcgc ttcaggtgcg
    10921 catccacctg acggatttcc gctgcggtgt tgtcgtaatg ctcttcctcc tccacatcca
    10981 gcgtgcgccc ttcctctgcg gctttggtca tgacctcctc aagggaggct gccagcgctg
    11041 cacgcttgtt ttcaaaactt ttaatctgtt cgccaatatt cattatggtc ttttccttat
    11101 gaaaaacggt tgttgactgt gccgcagcgc cggcagaaga tgcgattttc accaccggtt
    11161 tccggttgcc ggacgcggca gaaaacgggc ggtcgtaaga tttaatggtc cggatagtgc
    11221 attccgcatt cgcgggcacg gtgacggcag acacctccat cagctcccag cgcagaaaat
    11281 gcactccgcc tccgtccaga aaggtgtatt catgaggacg gaagcccacg gacagccccc
    11341 tgaccagccc ggtcttaatg gccgcccaga cctcatccag ccgggcagcc agttgcgacg
    11401 gcatatccgg tacgggtttc accagtgttg ccgtgatttc cagcccttcg ctgacccggc
    11461 gcaccgtaca ctgccccacc gggcgggaat ggtcatgctg ccagagaaac gggatcgcac
    11521 tgccaaactc cgcgccctcc ggctccagga tgtcaccatc ccgatccgga gaaggcgttg
    11581 acgcaatccc ggtgatcacc cgctcatcct cactgaagga tttcaccgtc agcagggagc
    11641 aggcccgttt aagagtcaca tcagcctcct gaaaataaaa aaaccgccgc agcggtccat
    11701 gatggttaca gggtgaacag ggttatatga aaaaaaccgc atattctttc tttttgggct
    11761 ccggattcag ggacatcagg gataccgcat tgaacagcgc catcagcggg tcaattttcc
    11821 cccgtccgct ggcctgtttg gtaataagga tggcgttacc tttaggctcc acccgggcat
    11881 tgccaacgca ccaggccatc agtggctgac caccatgcac cagcactccc tcagc
//
LOCUS       DAZKFU020000139        11915 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZKFU020000139 DAZKFU020000000
VERSION     DAZKFU020000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.140"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1325)
                     /gene="uidB"
                     /locus_tag="ACHOQQ_RS15535"
                     /old_locus_tag="pgaptmp_003107"
     CDS             complement(<1..1325)
                     /gene="uidB"
                     /locus_tag="ACHOQQ_RS15535"
                     /old_locus_tag="pgaptmp_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGU4471321.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEI"
     gene            complement(1322..3133)
                     /gene="uidA"
                     /locus_tag="ACHOQQ_RS15540"
                     /old_locus_tag="pgaptmp_003108"
     CDS             complement(1322..3133)
                     /gene="uidA"
                     /locus_tag="ACHOQQ_RS15540"
                     /old_locus_tag="pgaptmp_003108"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HGU4471322.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNYASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3524..4114)
                     /gene="uidR"
                     /locus_tag="ACHOQQ_RS15545"
                     /old_locus_tag="pgaptmp_003109"
     CDS             complement(3524..4114)
                     /gene="uidR"
                     /locus_tag="ACHOQQ_RS15545"
                     /old_locus_tag="pgaptmp_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGU4471323.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4343..5110)
                     /gene="hdhA"
                     /locus_tag="ACHOQQ_RS15550"
                     /old_locus_tag="pgaptmp_003110"
     CDS             complement(4343..5110)
                     /gene="hdhA"
                     /locus_tag="ACHOQQ_RS15550"
                     /old_locus_tag="pgaptmp_003110"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGU4471324.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5222..6250)
                     /gene="malI"
                     /locus_tag="ACHOQQ_RS15555"
                     /old_locus_tag="pgaptmp_003111"
     CDS             complement(5222..6250)
                     /gene="malI"
                     /locus_tag="ACHOQQ_RS15555"
                     /old_locus_tag="pgaptmp_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGU4471325.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6425..8017
                     /gene="malX"
                     /locus_tag="ACHOQQ_RS15560"
                     /old_locus_tag="pgaptmp_003112"
     CDS             6425..8017
                     /gene="malX"
                     /locus_tag="ACHOQQ_RS15560"
                     /old_locus_tag="pgaptmp_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGU4471326.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGIVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8027..9199
                     /gene="malY"
                     /locus_tag="ACHOQQ_RS15565"
                     /old_locus_tag="pgaptmp_003113"
     CDS             8027..9199
                     /gene="malY"
                     /locus_tag="ACHOQQ_RS15565"
                     /old_locus_tag="pgaptmp_003113"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGU4471327.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNATFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9303..10304
                     /gene="add"
                     /locus_tag="ACHOQQ_RS15570"
                     /old_locus_tag="pgaptmp_003114"
     CDS             9303..10304
                     /gene="add"
                     /locus_tag="ACHOQQ_RS15570"
                     /old_locus_tag="pgaptmp_003114"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGU4471328.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLDHGIRASIN
                     TDDPGVQGVDIIHEYNVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10339..11379)
                     /locus_tag="ACHOQQ_RS15575"
                     /old_locus_tag="pgaptmp_003115"
     CDS             complement(10339..11379)
                     /locus_tag="ACHOQQ_RS15575"
                     /old_locus_tag="pgaptmp_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4471329.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKSGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11622..11747
                     /gene="blr"
                     /locus_tag="ACHOQQ_RS15580"
                     /old_locus_tag="pgaptmp_003116"
     CDS             11622..11747
                     /gene="blr"
                     /locus_tag="ACHOQQ_RS15580"
                     /old_locus_tag="pgaptmp_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGU4471330.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
BASE COUNT         2896 a         3128 c         2911 g         2980 t
ORIGIN      
        1 tcaatttcaa ccacgatttc tttgaatttt ttatccgtga gcggataaaa ccagataata
       61 acgaatgcca gtagcataaa tccgcaaggt actaaggcaa ttgatgtgcg aatgcccata
      121 ataacttccg gcgtttgcac ctgattggcg atatatccgc ttaaccccaa aataaaggca
      181 ggaattgaac ctccgattgc ctgaccgcat ttacgggtaa atgagaatag tgaataggtg
      241 agcccttcaa ttcgcacgcc ggtcaggtat tcaccgtatt ctacggtatc agcttccagc
      301 gcccacataa cagtcatggt aacgccctga ccaattgaag cgatggccaa cgcaaccaac
      361 gccaccggca gtgaccagac ggaaacccag aagaatagca gataaccgca ggttcccagc
      421 aaagcgccaa tcaggaaggt attcttttta ccgatcctcg cgaccatccc cggcaccaac
      481 ggtgccgatg ccacagtacc aaccaggttt tgcaccagta ccagcacagt gaacagcccg
      541 gtatcattta acacatagcg cacgtagaac aacgacgagg cgctgaccgc gaaggtcgaa
      601 atcagcacac acagcgcacc gatgcacaac ataaacagcg ggcgattccg tttcagggtt
      661 tgcagactga tcttcaatga cggctgcgcc acgatacgta ccacattctc acgcgtcgat
      721 ttgaagcaga tgaagtaaag caccattccg gcaatcgcca gcacaatcgt ccagaaatgg
      781 tataccgaca ccatctcttc cgggctggag ttcttaatgc tcggtcctat cagaaatgcc
      841 aggcagacaa aggtcaatga agcggcaatc ccacgagccg cgcccagacg ggcgcgggat
      901 tgtggttgtt gggtcatcgc ggtagcaagt gaaccataag gaatattcac caggctgtag
      961 caaagcccga ggcccatgta ggtcaaatat gcatacacca ctttgctacc atggctccag
     1021 tcggtcggca cccagaatac cagcacgctg aagatcatta acggcgcagt accgaagagt
     1081 aaaaacgggc ggaattttcc ccagcgggta ttcacactgt ccaccactcg tccggcaaag
     1141 acgtcggcga aggcatcgaa tacccgcacc agtaacagca tggtgcccgc cgcagcggca
     1201 ccgacgccag cgacgtcggt gtagtaactt agcaggaaga gcgcccccat agcgaaggcg
     1261 aagttattgg cgacgtcacc gaggctgtag ccaacgatgg tgcgccagga gagttgttga
     1321 ttcattgttt gcctccctgc tgcggttttt caccgaagtt catgccagtc cagcgttttt
     1381 gcagcagaaa agccgccgac ttcggtttgc ggtcgcgggt gaagatgccc ttcttgttac
     1441 cgccaacgcg caatatgcct tgcgaggtcg caaaatcggc gaaattccat acctgttcac
     1501 cgacgacggc gctgacgcga tcaaagacgc ggtgatacat atccagccat gcacactgat
     1561 actcttcact ccacatgtcg gtgtacattg agtgcagccc ggctaacgta tccacgccgt
     1621 attcggtgat gataatcggc tgatgcagtt tctcctgcca ggccagaagt tctttttcca
     1681 gtaccttctc tgccgtttcc aaatcgccgc tttggacata ccatccgtaa taacggttca
     1741 ggcacagcac atcaaagaga tcgctgatgg tatcggtgtg agcgtcgcag aacattacat
     1801 tgacgcaggt gatcggacgc gtcgggtcga gtttacgcgt tgcttccgcc agtggcgcga
     1861 aatattcccg tgcaccttgc ggacgggtat ccggttcgtt ggcaatactc cacatcacca
     1921 cgcttgggtg gtttttgtca cgcgctatca gctctttaat cgcctgtaag tgcgcctgct
     1981 gagtttcccc gttgactgcc tcttcgctgt acagttcttt cggcttgttg cccgcttcga
     2041 aaccaatgcc taaagagagg ttaaagccaa cagctgcagt ttcatcaatc accacgatgc
     2101 catgttcatc tgcccagtcg agcatctctt cggcataagg gtaatgcgag gtacggtagg
     2161 agttggcccc aatccagtcc attaatgcgt gatcgtgcac catcagcacg ttatcgaatc
     2221 ctttgccgcg caaatccgca tcttcatgac ggccaaagcc agtaaagtag aacggtttgt
     2281 ggttgatcag gaactgttcg cccttcactg ccactgaccg gatgccgacg cgcagcgggt
     2341 agatatcaca ctctgtctgg cttttggctg tgacgcacag ttcatagaga taaccttcac
     2401 ccggttgcca gaggtgcgga ttcaccactt gcaaagtccc gctggtgcct tgtccagttg
     2461 caaccacctg ttgatccgca tcacgcagtt caacgctgac atcaccattg gccaccacct
     2521 gccagtcaac agacgcgtag ttacagtctt gcgcgacatg cgtcaccacg gtgatatcgt
     2581 ccacccaggt gttcggcgtg gtgtagagca ttacgctgcg atggatcccg gcgtagttaa
     2641 agaaatcatg gaagtaagac tgctttttct tgccgttttc gtcggtaatc accattcccg
     2701 gcgggatagt ctgccagttc agttcgttgt tcacacaaac ggtgatacgt acacttttcc
     2761 cggcaataac atacggcgtg acatcggctt caaatggcgt atagccgccc tgatgctcca
     2821 tcacttcctg attattgacc cacactttgc cgtaatgagt gaccgcatcg aaacgcagca
     2881 cgatacgctg gcctgcccaa cctttcggta taaagacttc gcgctgatac cagacgttgc
     2941 ccacataatt acgaatatct gcatcggcga actgatcgtt aaaactgcct ggcacagcaa
     3001 ttgcccggct ttcttgtaac gcgctttccc accaacgctg atcaattcca cagttttcgc
     3061 gatccagact gaatgcccac aggccgtcga gttttttgat ttcacgggtt ggggtttcta
     3121 caggacgtaa cataagggac tcctcattaa gataaaaata ctggtcaacc tttaatccga
     3181 ttagttaacc aattctgtgt tgcacacagt tatctgtgag aaacccatgg aagagaaatg
     3241 gaaataaaaa gaactgacac cgcgatcaaa aaaccaaaaa ctgcaatacc cacagcgtga
     3301 tatagatcgc attaatcttt aaaacggcgt tgacagaagc cataaaataa gcaatcgaac
     3361 gttcgttcga gcatattggg aacgataaac tctcatgaca tatggctaca gtgaatattt
     3421 tagggaggaa ttccccgctc cggcgagatt gtattttgta cagggaaatt aaagtatgaa
     3481 acgcgggctc agttgtcacc agcccgcgga aagagagaga gaatcaggat gcggttaaga
     3541 taccgccaat catcgcgcgt aatccctgag caaacgacgc ttcacgcgcc aggtcttccg
     3601 cttcgatatc ggccagtaca ccgtaggtca gatccagcag taaacgtgaa gtcattgccg
     3661 tgttgatgtc tgggcttatc tcgccttttt gctgggcatc acgcatccgc tgagcaacaa
     3721 attcagtgat cgtcatatgc ttatttttaa gcatggcggc gacctgtggg ttacgcatcc
     3781 cttccgccat aatttcgacc accagcgccc gttgcccaaa ggcttcatgg gtgagagaga
     3841 caatagactc gaccatatag tcaacaaaat gaatcccttc aatcggttcc cggaaacggg
     3901 ccagcgccct ctcctggtcc tgtaagataa tcgcctgaat caaggcttct ttggagatga
     3961 aatggtgata gagcgtcccg ggactaatgg cgcaggattt acagatggct ttcatcgagg
     4021 cactgtgaaa tccattttct gaaaaaatct ctctggcagc attgaggatc cgggtccgtg
     4081 tcggttgtgc ttcagtctgc atgttatcca tcattaaacc attatagtta gcgtgaataa
     4141 ctagtttacc gcgttgtcag gtgagcgcct atcattgctg ttgaatagtg cgaaggcaca
     4201 ctctattcgt atgcttttca tccagctatt ggttaaccaa tagccgtgat ttggctgcca
     4261 gttgtttagc agagacgttg atgaattgca cacgctatcc cggtaaacaa cacatacggc
     4321 tgtttaccgg ttcgttagtg tattaattga gctcctgtac cccaccaccg gagacggtga
     4381 gaatttgtcc gcttacccag ctggctgcag gcgagcaaag gaacagcgct gcgttagcaa
     4441 tatcttgcgg ttggcccaga cgtctgatcg gcgtgtgctg taacattttt tgttcaattt
     4501 ctggtgtaat aacggatttc agggcatcgg ttaatattgc ccccggcgca atgccattca
     4561 cccgaatatt tttttcaccc aggtcaaatg ccatatttct gaccagatga ctggccgcag
     4621 ctttagatga tgcataggaa gtcatgttta tatttttatt ttctgccgcc atagaagtga
     4681 tggtaagaat aacgccaccg ccattttttt ccatttctgg cgcaacaagt tgtgacagat
     4741 ggaaaaaaga aaacacattc agttcataag cacggcgaaa atccgccatt ggcatatcaa
     4801 acggtttagg tccaccgcca ccggcgttgt taaccagaat atcaacttta cccagcttac
     4861 tgatagcaaa gtctgccagt gcagagagtt cctgttcgga agtaatatca caacggcagg
     4921 caaatgcctg accacccagt tgttgaattt cgtctacaac atggttagct gcgtcggcgt
     4981 taatatcact gaccaccaca gatgcgccag ctgtcgcgaa tgtaatggcg atttctttac
     5041 caatacctgc acccgcacct gtgatgatgg cgcattttcc gtcgagtctc aggttgtcag
     5101 aattaaacac ataaacctcc tgtgaacttc agtaaaaata aaaggcaccg cctgtgtgcc
     5161 acatttttta tcattaacct tgaggaaagg acactaccag gtatagcaga taaagagagg
     5221 attatttcgc cgcaatgagc cgggcgggaa taataagatt gcgtgaatgc gtctcttcat
     5281 gggtgatttt ttgcatcatg cgatccgcaa gtgtggtacc aagttcccgc gcaggcgtgc
     5341 tggcccaggt aacgggtata tcatcaagtg tggttggtgt cgcatcggta aatgccgcca
     5401 gcgaaacctg ttgctcaaag taacgatcga ctccgctttc cccgctttgc cgccctgctt
     5461 tcagcaaacc aaaccatgcc cccatcgcaa tagtttcgtt atagcaaacc acggcactga
     5521 tggtcgggtt atgacgtaaa agcgccgtga tagcttccgc ggcttgcttc tggctggaag
     5581 tgcactccaa cacccaatcg ctgtgaaacg gcaggccaaa ttttagtaga gttgcacaat
     5641 agccccccac ccgttctgca cgggttaatg aggaactttg ccctcccagc caggcgatcc
     5701 gctgatgccc attgcgaatg agatgctccg tcaacaactg tgcagcctgc atgttgtccg
     5761 ggcgaaccgt atcaacatca tcaagataac tggcacggga agcgaaaatc accgggatag
     5821 ctttttcttc tgccatccgt cgcaggtcat cgctgcttcc tgcagccccg gcaattacca
     5881 caccatcgac accctgattc agtaacagtg aaaaccgctg tgccagctgc tcgccgtctt
     5941 taccgccgtg aagcaaaaaa accatccgtc cctgcgcttc cagagcttcc gtcaatccgg
     6001 ccgtcaattc ggcgtaaaac ggcgcagata aatcacggac gatcaaacca atgacgccgc
     6061 tttgcccgcc gcgcagcgcc gacgcctggc gattgcgcac aaatcccagc tcttcaatgg
     6121 cggcgttaac gcgttctcct gtggcggtag agattcgccc tttgccacta agcaccagcg
     6181 aaacggtact taccgacacg cccgcagcca gcgcaacatc atgaatggtt atttttttgg
     6241 cggtagccat gcagatgacc tactccctga ttttgtgaca gataaaacgt tttacctttt
     6301 atttatctta tacccgctat tatcgttgcg taatgtgatt tatgcctcac taaaatttga
     6361 taaaacgttt tatcttctcg cgcaatttac tgaatccaga ttgttctcta cgaggagtcg
     6421 ttttatgacg gcgaaaacag caccgaaagt cacgctgtgg gagttcttcc agcagttagg
     6481 caaaaccttc atgttacccg tggcattatt gtcgttctgc ggcattatgc tcggcattgg
     6541 tagttccctt agtagccacg atgtcatcac cctgatcccg gtcttgggca atcccgtgtt
     6601 gcaggctatc tttacctgga tgagtaagat tggctcgttt gcttttagtt tcctgccagt
     6661 catgttctgt attgccatcc cgctgggcct ggcacgcgaa aacaaaggcg tagcggcatt
     6721 cgctggcttc gtcggttatg cggtaatgaa cctcgcggta aacttctggt tgaccaataa
     6781 aggcattctg ccaaccacgg atgccgccgt tctgaaagcc aataacatcc agagcattct
     6841 tgggatccag tcgatcgaca ccgggatcct cggtgcggtg atcgccggta ttatcgtctg
     6901 gatgctgcat gagcgattcc ataatatccg cctgccggat gcgctggcat tcttcggcgg
     6961 tacgcgcttc gtaccaatca tctcctcgct ggtgatgggc attgtcggtc tggtgattcc
     7021 attagtctgg ccaattttcg ccatgggtat tagcggctta ggccatatga tcaatagcgc
     7081 gggtgatttc ggaccgatgc tgtttggtac cggtgaacgt ctgctgttgc cgtttggtct
     7141 gcatcacatt ctggtggcat taattcgctt taccgacgca ggcggcacgc aggaagtctg
     7201 cggtcaaacc gtcagcggtg cactgaccat cttccaggcg caattgagtt gcccgaccac
     7261 tcacggtttt tctgaaagcg ccacgcgttt cctttcgcaa ggcaaaatgc ctgcgtttct
     7321 cggcggtctg ccaggtgcag cgttagctat gtatcactgc gcgcgcccgg aaaatcgcca
     7381 taaaattaaa gggctgctga tttctggcct gatcgcctgt gtcgttggcg gcacgaccga
     7441 accgctggaa ttcctgttcc tgttcgtagc gccagttctg tatgtcatcc acgcgctgtt
     7501 aaccggcctc ggcttcaccg tcatgtctgt gctcggcgtc accatcggta ataccgacgg
     7561 caatatcatc gacttcgtgg tgttcggtat tttgcatggt ctgtcaacca agtggtacat
     7621 ggtgccagtg gtggcggcaa tctggtttgt cgtttactac gtcatcttcc gtttcgctat
     7681 cacccgcttc aatctgaaaa ccccggggcg cgatagcgaa gtggccagtt caatcgaaaa
     7741 agccgttgcc ggtgcgccgg gtaaatcagg ttacaacgtt ccggcaatcc tcgaagcctt
     7801 aggcggtgcc gacaatattg tcagccttga taactgcatt acccgtttgc gtttgtctgt
     7861 gaaagatatg tcgcttgtta atgtgcaggc actgaaggac aatcgggcaa ttggcgtagt
     7921 acaacttaat caacataacc tgcaggttgt tatcgggcca caagttcagt cagtaaaaga
     7981 tgaaatggcc ggtctgatgc atactgtcca ggcataagga taagatatgt tcgatttttc
     8041 aaaggtcgtg gatcgtcatg gcacatggtg tacgcagtgg gattatgtcg ctgaccgttt
     8101 cggcactgct gacctgttac ctttcacgat ttcagacatg gattttgcca ctgccccctg
     8161 cattatcgag gcgctgaatc agcgcctgat gcacggcgtg tttggctaca gccgctggaa
     8221 gaacgatgag tttctcgcgg ctattgccca ctggttttcc acccagcatt acaccgccat
     8281 cgatcctcag tcggtggtgt atggcccttc tgttatctat atggtctcag aactgattcg
     8341 tcagtggtct gaaacaggtg aaggcgtggt gatccacaca cccgcctatg acgcatttta
     8401 caaggccatt gaaggtaatc agcgcacagt aatgcccgtt gctttagaga agcaggctga
     8461 cggctggttt tgcgatatgg gcaagctgga agccgtgttg gcgaaaccag aatgtaaaat
     8521 tatgctcctg tgtagcccac agaatcctac cggaaaagtg tggacgtgcg atgagctgga
     8581 gatcatggct gacctgtgcg agcgtcatgg tgtgagggtt atttccgatg aaatccatat
     8641 ggatatggtt tggggcgagc agccgcatat tccctggagt aatgtggcgc ggggagactg
     8701 ggcgttgctg acgtcgggat cgaaaagttt caatattccc gcgctgaccg gtgcttacgg
     8761 gattatagaa aatagcagca gccgtgatgc ctatttatcg gcactgaaag gacgtgatgg
     8821 gctttcttcc ccttcggtac tggcgttaac tgcccatatc gccgcctatc agcaaggtgc
     8881 accgtggctg gatgccttac gcgtctatct gaaagataac ctgacgtata tcgcagataa
     8941 aatgaacgcc acgtttcctg aactcaactg gcagatcccg caatccactt atctggcctg
     9001 gcttgattta cgtccgttga atattgacga caacgccttg caaaaagcac ttatcgaaca
     9061 agaaaaagtc gcgatcatgc cggggtatac ctacggtgaa gaaggtcgtg gttttgtccg
     9121 actcaatgcc ggctgcccac gttcgaaact ggaaaaaggt gtggctggat taattaacgc
     9181 catccgcgct gttcgctaac cccaattgcg caacgtaaaa aatcgttgcg caatcgtgga
     9241 tttttaccct gctttgtttt tataatggtg cgcactttta tatccagaaa aagagtgcga
     9301 ccatgattga taccaccctg ccattaactg atattcatcg ccaccttgat ggcaacattc
     9361 gtccccagac cattcttgaa cttggccgcc agtataatat ctcgcttcct gcacaatccc
     9421 tggaaacact gattccccac gttcaggtca ttgccaacga acccgatctg gtgagctttc
     9481 tgaccaaact tgactggggc gttaaagttc tcgcctctct tgatgcttgt cgccgcgtgg
     9541 catttgaaaa cattgaagat gcagcccgtc acggcctgca ctatgtcgag ctgcgttttt
     9601 ctccaggcta catggcaatg gcacatcagc tgcctgttgc aggtgttgtt gaagcggtga
     9661 tcgatggcgt acgtgaaggt tgccgcacct ttggtgtgca ggcgaagctt atcggcatta
     9721 tgagccgaac cttcggcgaa gccgcctgtc agcaagagct ggaggccttt ttagcccacc
     9781 gtgaccagat taccgcactt gatttagccg gtgatgaact tggtttcccg ggaagtctgt
     9841 ttctttctca cttcaaccgc gcgcgtgatg cgggctggca tattaccgtc catgcaggcg
     9901 aagctgccgg gccggaaagc atctggcagg cgattcgtga actgggtgcg gagcgtattg
     9961 gacatggcgt aaaagccatt gaagatcggg cgctgatgga ttttctcgcc gagcaacaaa
    10021 ttggtattga atcctgtctg acctccaata ttcagaccag caccgtggct gagctggctg
    10081 cacatccgct gaaaacgttc cttgatcatg gcattcgtgc cagcattaac actgacgatc
    10141 ccggcgtaca gggagtggat atcattcacg aatataacgt tgccgcgcca gctgctgggt
    10201 tatcccgcga gcaaatccgc caggcacaga ttaatggtct ggaaatggct ttcctcagcg
    10261 ctgaggaaaa acgcgcactg cgagaaaaag tcgcagcgaa gtaacaaaaa tggatggtgc
    10321 aaattgcacc atccattttc acgcaaggca caaagtcgcg cgatgtttgg cggattcgat
    10381 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
    10441 atcgccattt aacgcatcac gaatagccgc atagtaagcc ggataattcc caggcaccgt
    10501 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
    10561 atcgtagccc cagtcttcct gcggcagacg ctcgccactt ttcagacgtt cttcctgtgg
    10621 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
    10681 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
    10741 gtggaaataa tcggtcgact gcgctccagg ccgtaactgt gccaagtcca ccgtcatgct
    10801 gaccggtaaa ccaaataatg taatagcctg atcaagaaga tgcggtgcta aatcgtacca
    10861 gataccactg cctgggccac cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
    10921 gtcaaaatga gactcaaagt aagcgacttc acccagcacg ccttccgcga gtaaaccttt
    10981 tagcgtcaag aaatcgctat cccagcgacg gttatggaat acagacagca cacgccccag
    11041 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
    11101 aacgaccaca tgtttacccg cctcaagcgc tgctttggct aacgggaaat gggtatcgtt
    11161 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
    11221 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
    11281 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
    11341 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
    11401 atgatggcta tttgacacta tcctttaccc tcgctcaaca gtttaataac ctgccagcaa
    11461 taagggatgt tgtttaactt aagtcaaaaa aatagcgatt tttccaacga caaaagctaa
    11521 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
    11581 ccctgaagtt aatggatcaa tcaacagaga tgtgagttgt aatgaatcgt cttattgaat
    11641 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
    11701 acaactatca gccacctgaa cagagtacta cggtacaaca caagtaagct ctgcgcttgt
    11761 agagcgacat gctgcccgtc agggtgcatg ttttcacttg tcggatatta aaccaggaat
    11821 ttattatctt gttcggtgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
    11881 catagcagtt tttcatccct gaatatttgc catca
//
LOCUS       DAZKFU020000140        11855 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZKFU020000140 DAZKFU020000000
VERSION     DAZKFU020000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.141"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..871
                     /locus_tag="ACHOQQ_RS15585"
                     /old_locus_tag="pgaptmp_003117"
     CDS             2..871
                     /locus_tag="ACHOQQ_RS15585"
                     /old_locus_tag="pgaptmp_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU4471331.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1064..1417)
                     /locus_tag="ACHOQQ_RS15590"
                     /old_locus_tag="pgaptmp_003118"
     CDS             complement(1064..1417)
                     /locus_tag="ACHOQQ_RS15590"
                     /old_locus_tag="pgaptmp_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGU4471332.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1555..3201)
                     /gene="groL"
                     /locus_tag="ACHOQQ_RS15595"
                     /old_locus_tag="pgaptmp_003119"
     CDS             complement(1555..3201)
                     /gene="groL"
                     /locus_tag="ACHOQQ_RS15595"
                     /old_locus_tag="pgaptmp_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGU4471333.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3245..3538)
                     /locus_tag="ACHOQQ_RS15600"
                     /old_locus_tag="pgaptmp_003120"
     CDS             complement(3245..3538)
                     /locus_tag="ACHOQQ_RS15600"
                     /old_locus_tag="pgaptmp_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGU4471334.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3814..5070
                     /gene="yjeH"
                     /locus_tag="ACHOQQ_RS15605"
                     /old_locus_tag="pgaptmp_003121"
     CDS             3814..5070
                     /gene="yjeH"
                     /locus_tag="ACHOQQ_RS15605"
                     /old_locus_tag="pgaptmp_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HGU4471335.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5086..5562)
                     /locus_tag="ACHOQQ_RS15610"
                     /old_locus_tag="pgaptmp_003122"
     CDS             complement(5086..5562)
                     /locus_tag="ACHOQQ_RS15610"
                     /old_locus_tag="pgaptmp_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGU4471336.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5899..7335
                     /gene="aspA"
                     /locus_tag="ACHOQQ_RS15615"
                     /old_locus_tag="pgaptmp_003123"
     CDS             5899..7335
                     /gene="aspA"
                     /locus_tag="ACHOQQ_RS15615"
                     /old_locus_tag="pgaptmp_003123"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGU4471337.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7453..8754
                     /gene="dcuA"
                     /locus_tag="ACHOQQ_RS15620"
                     /old_locus_tag="pgaptmp_003124"
     CDS             7453..8754
                     /gene="dcuA"
                     /locus_tag="ACHOQQ_RS15620"
                     /old_locus_tag="pgaptmp_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGU4471338.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8870..9208
                     /gene="cutA"
                     /locus_tag="ACHOQQ_RS15625"
                     /old_locus_tag="pgaptmp_003125"
     CDS             8870..9208
                     /gene="cutA"
                     /locus_tag="ACHOQQ_RS15625"
                     /old_locus_tag="pgaptmp_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGU4471339.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9184..10881
                     /gene="dsbD"
                     /locus_tag="ACHOQQ_RS15630"
                     /old_locus_tag="pgaptmp_003126"
     CDS             9184..10881
                     /gene="dsbD"
                     /locus_tag="ACHOQQ_RS15630"
                     /old_locus_tag="pgaptmp_003126"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGU4471340.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10918..11493
                     /locus_tag="ACHOQQ_RS15635"
                     /old_locus_tag="pgaptmp_003127"
     CDS             10918..11493
                     /locus_tag="ACHOQQ_RS15635"
                     /old_locus_tag="pgaptmp_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4471341.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11600..11675
                     /locus_tag="ACHOQQ_RS15640"
                     /old_locus_tag="pgaptmp_003128"
     tRNA            11600..11675
                     /locus_tag="ACHOQQ_RS15640"
                     /old_locus_tag="pgaptmp_003128"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11633..11635,aa:Phe,seq:gaa)
BASE COUNT         2826 a         3075 c         2942 g         3012 t
ORIGIN      
        1 catggccata agcatcaagg gtgttaatac cggtgttatt cgcaagagta ataatttcat
       61 tgcactggcg ctgaaaatta aagaacctcg caataaagaa tcgttgttct ttatgtctgt
      121 catggaactg cgcgacctgc tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact
      181 ggatgctgct gcacgccttc agtatgagca agctcgcgat aaagtcatta agaaaatggc
      241 agaaaatatc ccagaaattc tggttgatga gcttaaaaat gccgatatta atcgtcgggt
      301 taacacgctg gaacttaccg ataatcaagg cgaaaatctt accttcgtat taacgctaca
      361 tgacggtagc aaatgtgagt tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc
      421 aatcattcat gcaattaata atgcggaaat gcgtgaactg gcattacgta ttacctcact
      481 gttagatttc ctgccgttat acgacgttga ctgccagcaa aatggcaatc tggaatatga
      541 cacctattcc caaccggaat ggaaacataa cttgttcgac cattatctgg cggtgcttta
      601 ccgctttaaa gatgaaagtg gcaaagaaca atttagcggc gcagtggtga aaacgcgtga
      661 agcaacgccg ggtaaagaaa tagaggcgat cactcgccga atgcttgatt tcagcccacg
      721 actgaaaaaa ctcgcgggtg tgccatgtca ggtctatgtt cgcaccgtag cagcaaataa
      781 cgcccaaccg ctaacccagg atcaatgctt acgcgcgctg catcacttac gtgttcaatc
      841 caccagcaaa actgcaccac aggcgaaata atctcatctt tgctaatgcg aggctggagc
      901 catccagcct gaaatgactg ataacgtgat cgttggtcat gccggatgcg gcgtaaacgc
      961 cttatccggc ctacaaattc tcaagcattc aatcagttgc aatgcattat gtaggcctga
     1021 taagcgaagc gcatctggca attatacgtt tgtcaccatc acatcagttc gggcacttat
     1081 atacctggcc gataatctgg ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg
     1141 ggctactgat gccataaatc acgttaccgc ccattgcagc cgcctggttg cgcagatcgt
     1201 ttgctgcgcc gcgcatagaa ccgccctctt ctccgtgttg cccggaaagc cagttgcttt
     1261 gcttacctgt cgcagtacca atcagctggc actctgcgcc tggctgctcg tccacaatgc
     1321 gtacactctg accggcagca ctcaattcgt tgctggagct acaacccgcc atcagtagcg
     1381 cggcaccgac aatccctgct aagtatttta cgtgcatgtt attccccata atcaatgagc
     1441 tggacgtcac tcgcgtcgtc cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa
     1501 acccccagac atttctgccc gggggtttgt ttatttctgc gaggtgcagg gcaattacat
     1561 catgccgccc atgccaccca tgccgcccat accgccagca gcgcctaagt cagctgcatc
     1621 gtttttcggc aggtcggtaa ccatgcactc ggtggtgatc atcaggccag ccacagaagc
     1681 cgcgtactgc agagcagaac gggttacttt ggttggatcc aggataccca tgtcgatcat
     1741 gttgccgtat tcttcggttg ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt
     1801 gttagcaaca acagacggtt cttcgccgca gttcaggacg atctgacgca gcggagcttc
     1861 cattgcacgc agtgcaactt tgatacccac gttctggtct tcgttctgac cacgcaggtc
     1921 agccagttta gacgctacgc ggatcagcgc aacaccacca ccagcaacca cgccttcttc
     1981 taccgcagca cgggtcgcgt gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc
     2041 aacttcggta gcagcaccta ctttgataac tgcaacgccg cctgccagtt tcgctacgcg
     2101 ctcctgcagt ttttcacggt cgtagtcaga agttgcttct tcaatctgct gacggatctg
     2161 agcaacacgg ccctggattg cagcttcttc acccacgcca tcgatgatag tggtggtgtc
     2221 tttgttgatc acaacacgtt tagcctgacc caggtcttcc agggttgctt tttccagctc
     2281 cataccgatc tcttcagaga ttacggtacc gccagtcagg gttgcgatat cctgcagcat
     2341 agctttacga cgatcgccga agcccggagc tttaactgca gcaactttca cgatgccacg
     2401 catggtgtta acaaccagag ttgccagcgc ttcgccttct acatcttcag cgatgatcag
     2461 cagcggtttg cctgctttgg caacggcttc cagaaccggc agcatttcgc ggatgttgga
     2521 gattttcttg tcagccagca ggatgaacgg gctttccagt tctactgcgc cagtttccgg
     2581 cttgttgatg aagtaaggag acaggtagcc acggtcgaac tgcatacctt caaccacgtc
     2641 cagttcgtcc tgcagaccgg taccgtcttc aacggtgata acgccttctt taccgacttt
     2701 gtccatcgct tcagcgatca gtttacctac ggtttcgtcg gagttagcgg agatagtacc
     2761 aacctgagca atcgctttag agtcagagca cggtacggac agcgctttca gttcttcaac
     2821 tgcagcggta acagctttgt cgataccacg tttcaggtcc atcgggttca tgcccgcagc
     2881 aacagctttc agaccttcag tgatgatagc ctgagccagt acggttgcag tggtggtacc
     2941 gtcgcctgca gcgtcgttcg ctttagaggc aacttctttc accatctgcg cacccatgtt
     3001 ttcgaacttg tcttccagtt cgatttcacg agcaacggaa acaccatctt tggtgatggt
     3061 cggtgcaccg aaagatttat ccagaactac gttacggcct ttcggaccga gggtaacttt
     3121 cactgcatct gccagtacgt ttacgccgcg cagcattttc acacgagcgt cgttaccgaa
     3181 ttttacgtct ttagctgcca ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg
     3241 aggattacgc ttcaacaatt gccagaatgt cgctttcgga catgatcaac acttcttcat
     3301 tgtcgatctt ctcggatttc acaccgtagc catcgttgaa aataacgatg tcgccaactt
     3361 tcacatccag cggcttcact tcgccatttt caaggatacg gccattgccg acagccagca
     3421 cttcgccgcg ggtggattta gccgctgcag agccggtcag aacgatgccg ccagcagatt
     3481 tagtttcaac ttctttacgc ttgacgatca cgcgatcatg caatggacga atattcattg
     3541 ataactctcc tttgagaaag tccgtatctg ttatgggtga cgccggagct tacgtggttt
     3601 cccggctggt gaccagagaa atggggatga ggcttcgccc cttcaagggg gaaaaagaaa
     3661 aaaaattctg atcagcacaa aatcgggtga aaaccctgat tcacctcaca tttcatcgca
     3721 atttcttcat cgccgtcata agcgaatctg attgtgctac catcgaaaat ctacgcagtt
     3781 gcccaaaatt tgggcgcaat cggacatcaa cccatgagtg gactcaaaca agaactgggg
     3841 ctggcccagg gcattggcct gctatcgacg tcattattag gcactggcgt gtttgccgtt
     3901 cctgcgttag ctgcactggt agcgggcaat aacagcctgt gggcgtggcc cgttttgatt
     3961 atcttagtgt tcccgattgc gattgtgttt gcgattctgg gtcgccacta tcccagcgca
     4021 ggcggcgtcg cgcacttcgt cggtatggcg tttggttcgc ggcttgagcg agtcaccggc
     4081 tggctgtttt tatcggtcat tcccgtgggt ttgcctgccg cgctacaaat tgctgccgga
     4141 ttcggccagg caatgtttgg ctggcatagc tggcaactgt tgttggcaga actcggtacg
     4201 ctggcgttgg tgtggtatat cggtactcgc ggtgccagtt ccagtgcaaa tctacaaacc
     4261 gttattgccg gacttatcgt cgcactgatt gtcgctatct ggtgggcggg cgatatcaaa
     4321 cctgcgagta tccccttccc cgcgccagga aatatcgaac ttaccgggtt atttgctgcg
     4381 ttatcagtga tgttctggtg ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa
     4441 tttaaaaatc cagaacgtga ttttcctcgt gctttgatga ttggtctgct gctggcagga
     4501 ttagtctact ggggctgtac ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg
     4561 gccgcggcag catcgcttcc aaaaattgta gtacaactgt tcggtgtagg agcgttatgg
     4621 attgcctgcg taattggcta tctggcctgc tttgccagtc tcaacattta tatacagagc
     4681 ttcgcccgcc tggtctggtc gcaggcgcaa cataatcctg accactacct ggcacgcctc
     4741 tcttctcgcc atatcccgaa taatgccctc aatgcggtgc tcggctgctg cgtggtgagc
     4801 acgttggtga ttcatgcttt agagatcaat ctggacgctc ttattattta tgccaatggc
     4861 atctttatta tgatttatct gttatgcatg ctggcaggct gtaaattatt gcaaggacgt
     4921 tatcgactac tggcggtggt tggcgggctg ttatgcgttc tgttactggc aatggtcggc
     4981 tggaaaagtc tctatgcgct gatcatgctg gcggggttat ggctgtttct gccaaaacga
     5041 aaaacgccgg aaaatggcat aaccacataa tccggcgttt cgacattaat cctggcgatc
     5101 gtctttatga tcaaggcggt cgcgctcatc atcctttcgc tggtactcac catcaaaagt
     5161 attaccgcca ccggtcccgg cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg
     5221 tggcatcaac ttcactgtca gatgcttttg caccggcggc aataaaagca gaagaccgag
     5281 gaagtcggtg aaaaagcccg gtaataaaag cagcaaaccg gcaatgatca gcgaaacact
     5341 tttaatcatc tccgccgctg ggttttcacc cgccgccatt ttttgctgca tcagcacgaa
     5401 attcttaaag ccctggttac gtaccagtga cataccgata actgacgtga atataaccag
     5461 cacgagggtc aacaataccc ccaatacatg ggcaacctga ataaagattg aaatctcaat
     5521 atagacataa aggaaaatgg caataaaagg taaccagcgc aaaggtttct cctgtaatag
     5581 cagccggtta aacccggcta cctgaatggg ttgcgaatcg cgttcagctt atattgtggt
     5641 cattagcaaa atttcaagat gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga
     5701 tctatttcac aaattaataa ttaaggggta aaaaccgaca cttaaagtga tccagattac
     5761 ggtagaaatc ctcaagcagc atatgatctc gggtattcgg tcgatgcagg ggataatcgt
     5821 cggtcgaaaa acattcgaaa ccacatatat tctgtgtgtt taaagcaaat cattggcagc
     5881 ttgaaaaaga aggttcacat gtcaaacaac attcgtatcg aagaagatct gttgggtacc
     5941 agggaagttc cagctgatgc ctactatggt gttcacactc tgagagcgat tgaaaacttc
     6001 tatatcagca acaacaaaat cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt
     6061 aaaaaagccg cagctatggc aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat
     6121 gccatcattg ccgcatgtga tgaagtcctg aacaacggaa aatgcatgga tcagttcccg
     6181 gtagacgtct accagggcgg cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg
     6241 gccaatatcg gtctggaact gatgggtcac cagaaaggtg aatatcagta cctgaacccg
     6301 aacgaccatg ttaacaaatg tcagtccact aacgacgcct acccgaccgg tttccgtatc
     6361 gcagtttact cttccctgat taagctggta gatgcgatta accaactgcg tgaaggcttt
     6421 gaacgtaaag ctgtcgaatt ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac
     6481 gcagtaccga tgaccctcgg tcaggaattc cgcgctttca gcatcctgct gaaagaagaa
     6541 gtgaaaaaca tccaacgtac cgctgaactg ctgctggaag ttaaccttgg cgcaacagca
     6601 atcggtactg gtctgaacac gccgaaagag tactctccgc tggcagtgaa aaaactggct
     6661 gaagttactg gcttcccatg cgtaccggct gaagacctga tcgaagcgac ctctgactgc
     6721 ggcgcttatg ttatggttca cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc
     6781 tgtaacgacc tgcgcttgct ctcttctggt ccacgtgccg gcctgaacga gatcaacctg
     6841 ccggaactgc aggcgggctc ttccatcatg ccagctaaag taaacccggt tgttccggaa
     6901 gtggttaacc aggtatgctt caaagtcatc ggtaacgaca ccactgttac catggcagca
     6961 gaagcaggtc agctgcagtt gaacgttatg gagccggtca ttggccaggc catgttcgaa
     7021 tccgttcaca ttctgaccaa cgcttgctac aacctgctgg aaaaatgcat taacggcatc
     7081 actgctaaca aagaagtgtg cgaaggttac gtttacaact ctatcggtat cgttacttac
     7141 ctgaacccgt tcatcggtca ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc
     7201 ggtaagagtg tacgtgaagt cgttctggaa cgcggtctgt tgactgaagc ggaacttgac
     7261 gatattttct ccgtacagaa tctgatgcac ccggcttaca aagcaaaacg ctatactgat
     7321 gaaagcgaac agtaatcgta cagggtagta caaataaaaa aggcacgtca gatgacgtgc
     7381 cttttttctt gtgagcagta acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca
     7441 aggaaggcta atatgctagt tgtagaactc atcatagttt tgctggcgat cttcttgggc
     7501 gccagattgg ggggaatagg tattggtttt gcaggcggat tgggggtgct ggttcttgcc
     7561 gctattggcg ttaaacccgg taacatccca ttcgatgtca tttccattat catggcggtt
     7621 atcgccgcta tttctgccat gcaggttgct ggcggtctgg actatctggt tcatcagaca
     7681 gaaaagctgc tgcgccgtaa cccgaaatac atcacgatcc tcgcaccgat cgtgacctat
     7741 ttcctgacta tctttgctgg tactggcaac atctctctgg cgacactgcc agttatcgct
     7801 gaagttgcga aggaacaagg cgttaaacct tgccgtccgc tgtctactgc agtggtatcc
     7861 gcgcagattg cgatcaccgc atcgccaatc tcagcggcag tggtttacat gtcttccgtg
     7921 atggaaggtc atggcatcag ctacctccat ctgctctccg tggtcatccc gtccaccctg
     7981 ctggcggttc tggtgatgtc cttcctggtc actatgctgt tcaactccaa actctctgac
     8041 gatccgattt atcgcaagcg tctggaagag ggcctggttg aactgcgcgg tgaaaagcag
     8101 attgaaatca aatccggtgc aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc
     8161 gtggttatct atgcaatcat caacagccca agcatgggtc tggttgaaaa accgctgatg
     8221 aacaccacca acgcaatcct gatcatcatg ctcagcgttg caactctgac caccgttatc
     8281 tgtaaagtcg ataccgacaa catcctcaac tccagcacct tcaaagcagg tatgagcgcc
     8341 tgtatttgta tcctgggtgt tgcgtggctg ggcgatactt tcgtttccaa caacatcgac
     8401 tggatcaaag ataccgctgg tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc
     8461 ttcttctttg cttctgctct gctgtactct caggctgcaa ccgcaaaagc actgatgccg
     8521 atggctctgg cactgaacgt ttctccgctg accgctgttg cttctttcgc tgcggtgtct
     8581 ggtctgttca ttctgccgac ctacccgacg ctggttgctg cggtacagat ggatgacacg
     8641 ggtactaccc gtatcggtaa attcgtcttc aaccatccgt tcttcatccc gggtactctg
     8701 ggtgttgccc tggccgtttg cttcggcttc gtgctgggta gcttcatgct gtaatgacca
     8761 atcgcggggc gttcacgccc cgctttcttt cccgccgact aacatccttc ccccgtccgt
     8821 tgtatagtga cctctctctt gcggttccat ctgttcttgc gaggtgttta tgcttgatga
     8881 aaaaagttcg aataccgcgt ctgtcgtggt gctatgtacg gcaccagatg aagcgacagc
     8941 ccaggattta gccgccaaag tgctggcgga aaaactggcg gcctgcgcga ccttgatccc
     9001 cggcgctacc tctctctatt actgggaagg taagctggag caagaatacg aagtgcagat
     9061 gattttaaaa actaccgtat ctcaccagca ggcactgctg gaatgcctga agtctcatca
     9121 tccatatcaa accccggaac ttctggtttt acctgttaca cacggagaca cagattacct
     9181 ctcatggctc aacgcatctt tacgctgatc ctgctacttt gcagcacttc cgtttttgcc
     9241 ggattattcg acgcgccggg acgttcacaa tttgtccccg cggatcaagc ctttgctttt
     9301 gattttcagc aaaaccaaca tgaccttaat ctgacctggc agatcaaaga cggttactac
     9361 ctctaccgta aacagatccg cattacgccg gaacacgcga aaattgccga cgtgcagctg
     9421 ccgcaaggcg tctggcatga agatgagttt tacggcaaaa gcgagattta ccgcgatcgg
     9481 ctgacgcttc ccgtaaccat aaaccaggcg agtgcgggag cgacgttaac tgtcacctac
     9541 cagggctgtg ctgatgccgg tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc
     9601 gaagtggtcg ccaacaacgc agcgtcacag cctgtgtctg tttcgcagca agagcagcac
     9661 accgcgcaat tgcccttttc cgcgctctgg gcgttgttga tcggtattgg tatcgccttt
     9721 acgccatgcg tgctgccaat gtacccactg atttctggca tcgtgctggg cggtaaacag
     9781 cggctctcca ctgccagagc attgttgctg acctttattt atgtgcaggg gatggcgctg
     9841 acctacacgg cgctgggtct ggtggttgcc gccgcagggt tacagttcca ggcggcgcta
     9901 cagcacccat acgtgctcat tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg
     9961 tttggcttgt ttactctaca actcccctcc tcgctgcaaa cgcgcctcac gctgatgagc
    10021 aatcgccaac agggcggctc acccggcgga gtgtttatta tgggggcgat tgccggactg
    10081 atctgttcac catgcaccac cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc
    10141 gggaacatgt ggctgggcgg cggcacgctt tatctctatg cgttgggcat gggcctgccg
    10201 ctgatgctaa ttaccgtctt tggtaaccgc ttgctgccga aaagcggccc gtggatggaa
    10261 caagtcaaaa ccgcgtttgg ttttgtgatc ctcgcactgc cggtcttcct gctggagcga
    10321 gtgattggtg atatatgggg attacgcttg tggtcggcgc tgggtgtcgc attctttggc
    10381 tgggccttta tcaccagcct acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc
    10441 ctgctggcag cggcattggt tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg
    10501 cataccgcgc aaactcagac gcatctcaac tttacacaaa tcaaaacggt agatgagtta
    10561 aatcaggcgc tcgttgaagc caaaggcaaa ccggtgatgt tagatcttta tgccgactgg
    10621 tgcgtcgcct gtaaagagtt tgagaaatac accttcagcg acccgcaggt gcaaaaagcg
    10681 ttagcagaca cggtcttact tcaggccaac gtcacggcca acgacgcaca agatgtggcg
    10741 ctgttaaagc atcttaatgt ccttggccta ccgacaattc tcttttttga cggacaaggc
    10801 caggagcatc cacaagcacg cgtcacgggc tttatggatg ctgaaacctt cagcgcacat
    10861 ttgcgcgatc gccaaccgtg aacgacactt gcagtgggaa agacggagga aaatagcgtg
    10921 caacgtgaag atgtactggg agaagccctg aaattattag aattacaagg gattgccaac
    10981 accacgctgg agatggttgc tgaacgtgtg gattatccac tggacgagct gcgccgcttc
    11041 tggccagaca aagaggcaat cctctacgat gcgctgcgtt atcttagcca acagatagat
    11101 gtctggcgtc gtcagctgat gctggacgaa acgcaaaccg ccgaacaaaa gctgctggca
    11161 cgttatcagg cgttatcgga gtgcgttaaa aacaaccgct atccgggctg tctatttatc
    11221 gctgcctgta cgttttatcc cgatcctggc caccctattc atcaactggc cgatcagcaa
    11281 aaaagcgcag cctacgattt cacccacgaa ctgttaacca cgctggaagt tgacgatccg
    11341 gcgatggtag caaagcagat ggaactggtg ctggaaggct gtttaagccg aatgctagtg
    11401 aatcgtagcc aggcggatgt cgacactgca catcggctgg cggaagatat cctgcgcttc
    11461 gcccgctgcc gccagggtgg cgcattgacc tgacagaaac acagaaaaga agcgatttgc
    11521 cgcaatctta agcagttgaa tcgcttttac tgaaattagg ttgacgagat gtgcagatta
    11581 cggtttaatg cgccccgttg cccggatagc tcagtcggta gagcagggga ttgaaaatcc
    11641 ccgtgtcggt ggttcgattc cgcctccggg caccatatac cagaacacgc tggttaaggg
    11701 atgagatcct tgacgggcgg ttaatacagg atgcggcatc aaagggtctc cgtttgtgta
    11761 agttgtggtt acactgattg agttcggtat cgtatcctca aacagggtgt cttttgcaga
    11821 gacgccctgt tctcgttaac actgtatgct tacat
//
LOCUS       DAZKFU020000141        11718 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZKFU020000141 DAZKFU020000000
VERSION     DAZKFU020000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.142"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..394)
                     /locus_tag="ACHOQQ_RS15645"
                     /old_locus_tag="pgaptmp_003129"
     CDS             complement(<1..394)
                     /locus_tag="ACHOQQ_RS15645"
                     /old_locus_tag="pgaptmp_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471342.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDTPTPSSEPINHFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAE"
     gene            917..2845
                     /gene="thrS"
                     /locus_tag="ACHOQQ_RS15650"
                     /old_locus_tag="pgaptmp_003130"
     CDS             917..2845
                     /gene="thrS"
                     /locus_tag="ACHOQQ_RS15650"
                     /old_locus_tag="pgaptmp_003130"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGU4471343.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2849..3391
                     /gene="infC"
                     /locus_tag="ACHOQQ_RS15655"
                     /old_locus_tag="pgaptmp_003131"
     CDS             2849..3391
                     /gene="infC"
                     /locus_tag="ACHOQQ_RS15655"
                     /old_locus_tag="pgaptmp_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGU4471344.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3488..3685
                     /gene="rpmI"
                     /locus_tag="ACHOQQ_RS15660"
                     /old_locus_tag="pgaptmp_003132"
     CDS             3488..3685
                     /gene="rpmI"
                     /locus_tag="ACHOQQ_RS15660"
                     /old_locus_tag="pgaptmp_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGU4471345.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3738..4094
                     /gene="rplT"
                     /locus_tag="ACHOQQ_RS15665"
                     /old_locus_tag="pgaptmp_003133"
     CDS             3738..4094
                     /gene="rplT"
                     /locus_tag="ACHOQQ_RS15665"
                     /old_locus_tag="pgaptmp_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGU4471346.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4211..4335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4217..4261
                     /gene="pheM"
                     /locus_tag="ACHOQQ_RS15670"
                     /old_locus_tag="pgaptmp_003134"
     CDS             4217..4261
                     /gene="pheM"
                     /locus_tag="ACHOQQ_RS15670"
                     /old_locus_tag="pgaptmp_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGU4471347.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4544..5527
                     /gene="pheS"
                     /locus_tag="ACHOQQ_RS15675"
                     /old_locus_tag="pgaptmp_003135"
     CDS             4544..5527
                     /gene="pheS"
                     /locus_tag="ACHOQQ_RS15675"
                     /old_locus_tag="pgaptmp_003135"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGU4471348.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5542..7929
                     /gene="pheT"
                     /locus_tag="ACHOQQ_RS15680"
                     /old_locus_tag="pgaptmp_003136"
     CDS             5542..7929
                     /gene="pheT"
                     /locus_tag="ACHOQQ_RS15680"
                     /old_locus_tag="pgaptmp_003136"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGU4471349.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITSEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7934..8233
                     /gene="ihfA"
                     /locus_tag="ACHOQQ_RS15685"
                     /old_locus_tag="pgaptmp_003137"
     CDS             7934..8233
                     /gene="ihfA"
                     /locus_tag="ACHOQQ_RS15685"
                     /old_locus_tag="pgaptmp_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGU4471350.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8334..9314
                     /gene="btuC"
                     /locus_tag="ACHOQQ_RS15690"
                     /old_locus_tag="pgaptmp_003138"
     CDS             8334..9314
                     /gene="btuC"
                     /locus_tag="ACHOQQ_RS15690"
                     /old_locus_tag="pgaptmp_003138"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGU4471351.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9377..9928
                     /gene="btuE"
                     /locus_tag="ACHOQQ_RS15695"
                     /old_locus_tag="pgaptmp_003139"
     CDS             9377..9928
                     /gene="btuE"
                     /locus_tag="ACHOQQ_RS15695"
                     /old_locus_tag="pgaptmp_003139"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGU4471352.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKFAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9928..10677
                     /gene="btuD"
                     /locus_tag="ACHOQQ_RS15700"
                     /old_locus_tag="pgaptmp_003140"
     CDS             9928..10677
                     /gene="btuD"
                     /locus_tag="ACHOQQ_RS15700"
                     /old_locus_tag="pgaptmp_003140"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGU4471353.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEIRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10756..11220
                     /gene="nlpC"
                     /locus_tag="ACHOQQ_RS15705"
                     /old_locus_tag="pgaptmp_003141"
     CDS             10756..11220
                     /gene="nlpC"
                     /locus_tag="ACHOQQ_RS15705"
                     /old_locus_tag="pgaptmp_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4471354.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11467..>11718
                     /gene="ydiV"
                     /locus_tag="ACHOQQ_RS15710"
                     /old_locus_tag="pgaptmp_003142"
     CDS             11467..>11718
                     /gene="ydiV"
                     /locus_tag="ACHOQQ_RS15710"
                     /old_locus_tag="pgaptmp_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU4471355.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWL"
BASE COUNT         2964 a         2708 c         3135 g         2911 t
ORIGIN      
        1 tttcggcagt taactgttcc tgttcaatat aatccagtat gcttcgcaaa tggttagctt
       61 taataggcgt ggtttccagt aataaggaaa tgatttcttt tttatagtgt aatgtaataa
      121 gtatattttc tgcatggatg ggtgaagtgg caaagttaat ggtaaaaagg gcagtggaat
      181 aacaattttg tgatattttt cgacagtttg cggtaaattc ctgagtaaca cagtatgaca
      241 gcatcgaact taagtcagag gtatcaagca gggtgatgag ctggcgcgca aaatgattaa
      301 taggctctga tgaaggcgtt ggtgtgtcgc tatcgatgtg cattctatga ggagacttat
      361 aatgagttct gataataatg tcgttttgac tcataatctt ttcgccttat atatgtctgg
      421 aggttttgag gatgatgatt aattattatc gccagaacga aatgtgcacg aaaaattgct
      481 ataagaattg tgtcactttt atcattgtga tatgaccact tcgtcattaa tctcatgatc
      541 gtttaaatac taccgccaaa tcatgcaacg aactttgctg gatttagttt atgctgctgt
      601 aaatccgctc gcagtaaacc tttcagacgc tccgtgatgt tatcagttgt tctttaagcg
      661 ttttgctggt gtactcacta caaacgaatt gcgaatcgaa tcaatgtgaa acggaaaggt
      721 acaatctccc tttttttagt gttaacatcg ctcaaccggg tggaaggtga gaggttaatg
      781 tctttcattt gtgtacctga ttgcgaacca atttagcatt tgttggctag atggtttcgc
      841 aatgaactgt taataaacaa atttttcttt gtatgtgatc tttcgtgtgg gtcaccactg
      901 caaataagga tataaaatgc ctgttataac tcttcctgat ggcagccaac gccattacga
      961 ccacgctgta agccccatgg atgttgcgct ggacattggt ccaggtctgg cgaaagcctg
     1021 tatcgcaggg cgcgttaatg gcgaactggt tgatgcttgc gatctgattg aaaacgacgc
     1081 acaactgtcg atcatcaccg ccaaagacga agaaggtctg gagatcattc gtcactcctg
     1141 tgcgcacctg ttagggcacg cgattaaaca actttggccg cataccaaaa tggcaatcgg
     1201 cccggttatt gacaacggtt tttattacga cgttgatctt gaccgcacgt taacccagga
     1261 agatgtcgaa gcactcgaga agcggatgca tgagcttgct gagaaaaact acgacgtcat
     1321 taagaagaaa gtcagctggc acgaagcgcg tgaaactttc gccaaccgtg gggagagcta
     1381 caaagtctcc attcttgacg aaaacatcgc ccatgatgac aagccaggtc tgtacttcca
     1441 tgaagaatat gtcgatatgt gccgcggccc gcacgtaccg aacatgcgtt tctgccatca
     1501 tttcaaacta atgaaaacgg caggggctta ctggcgtggc gacagcaaca acaaaatgtt
     1561 gcaacgtatt tacggtacgg cgtgggcaga caaaaaagcg cttaacgctt acctgcagcg
     1621 cctggaagaa gccgcgaaac gcgaccaccg taaaatcggt aaacagctcg acctgtacca
     1681 tatgcaggaa gaagcgccgg gtatggtatt ctggcacaac gacggctgga ccatcttccg
     1741 tgaactggaa gtgtttgttc gttctaaact gaaagagtac cagtatcagg aagttaaagg
     1801 tccgttcatg atggaccgtg tcctgtggga aaaaaccggt cactgggaca actacaaaga
     1861 tgcaatgttc accacgtctt ctgagaaccg tgaatactgc attaagccga tgaactgccc
     1921 gggtcacgta caaattttta accaggggct gaagtcttat cgcgatctgc cgctgcgtat
     1981 ggccgagttt ggtagctgcc accgtaacga gccgtcaggt tcgctgcatg gcctgatgcg
     2041 cgtgcgtgga tttacccagg atgacgcgca tatcttctgt actgaagaac aaattcgcga
     2101 tgaagttaac ggatgtatcc gtttagtcta tgatatgtac agcacttttg gcttcgagaa
     2161 gatcgtcgtc aaactctcca ctcgtcctga aaaacgtatt ggcagcgacg aaatgtggga
     2221 tcgtgctgag gcggacctgg cggttgcgct ggaagaaaac aacatcccgt ttgaatatca
     2281 actgggtgaa ggcgctttct acggtccgaa aattgaattt accctgtatg actgcctcga
     2341 tcgtgcatgg cagtgcggta cagtacagct ggacttctcc ttgccgtctc gtctgagcgc
     2401 ttcttatgta ggcgaagaca atgaacgtaa agtaccggta atgattcacc gcgcaattct
     2461 ggggtcgatg gaacgtttca tcggtatcct gaccgaagag ttcgctggtt tcttcccgac
     2521 ctggcttgcg ccggttcagg ttgttatcat gaatattacc gattcacagt ctgaatacgt
     2581 taacgaattg acgcaaaaac tatcaaatgc gggcattcgt gttaaagcag acttgagaaa
     2641 tgagaagatt ggctttaaaa tccgcgagca cactttgcgt cgcgtcccat atatgctggt
     2701 ctgtggtgat aaagaggtgg aatcaggcaa agttgccgtt cgcacccgcc gtggtaaaga
     2761 cctgggaagc atggacgtaa atgaagtgat cgagaagctg caacaagaga ttcgcagccg
     2821 cagtcttaaa caattggagg aataaggtat taaaggcgga aaacgagttc aaacggcgcg
     2881 ccctaaccgt atcaatggcg aaattcgcgc ccaggaagtt cgcttaacag gtctggaagg
     2941 cgagcagctt ggtattgtga gtctgagaga agctctggag aaagcagaag aagccggagt
     3001 agacttagtc gagatcagcc ctaacgccga gccgccggtt tgtcgtataa tggattacgg
     3061 caaattcctc tatgaaaaga gcaagtcttc taaggaacag aagaaaaagc aaaaagttat
     3121 ccaggttaag gaaattaaat tccgtcctgg tacagatgaa ggcgactatc aggtaaaact
     3181 ccgcagcctg attcgctttc tcgaagaggg tgataaagcc aaaatcacgc tgcgtttccg
     3241 cggtcgtgag atggcgcacc agcaaatcgg tatggaagtg cttaaccgcg tgaaagacga
     3301 tttgcaagaa ctggcagtgg tcgaatcctt cccaacgaag atcgaaggcc gccagatgat
     3361 catggtgctc gctcctaaga agaaacagta aggccttcaa gtagcactgt ctgtggagcc
     3421 ttcgggcttc acagactttg ttcgcctttg tttcgtttat taacaatgcg aagtggaagt
     3481 tattaaaatg ccaaaaatta agaccgtacg cggtgctgct aagcgcttca aaaaaaccgg
     3541 taaaggtggt tttaagcaca agcacgctaa cctgcgtcac attctgacca aaaaagcgac
     3601 caaacgtaaa cgtcacctgc gtccgaaagc catggtttcc aaaggcgatc tgggcctggt
     3661 aatcgcgtgc ctgccgtacg cataagccgt taacgttttt aactttttaa ttagaatata
     3721 gatacaggag agcacatatg gctcgcgtaa aacgtggtgt tattgcacgc gcacgtcaca
     3781 agaaaatttt gaaacaagct aaaggctact acggtgcgcg ttctcgcgta taccgcgttg
     3841 ccttccaggc tgttatcaaa gctggtcagt atgcttaccg tgaccgtcgt caacgtaagc
     3901 gtcagttccg tcaactgtgg attgcgcgta tcaacgcagc agcacgtcag aacggtattt
     3961 cttacagcaa attcatcaat ggcctgaaaa aagcctctgt tgaaatcgac cgtaagatcc
     4021 tggctgatat cgcagtattc gacaaagtag cgttcaccgc tctggttgaa aaagcgaaag
     4081 cagctctggc ataagccagt tgaaagaggg agctagtctc cctcttttcg tttcaacgcc
     4141 atcaaaacat tgacttttat cgccgtagcc ttttcaataa aggtcttttg aagagtacca
     4201 aaaggtaacg caagcaatga atgctgctat tttccgcttc tttttttact ttagcacctg
     4261 aatccaggag gctagcgcgt gagaagagaa acggaaaaca gcgcctgaaa gcctcccagt
     4321 ggaggctttt tttgtatgcg cgttttgaaa aataaaaagc cagtctatca ggcgtaattg
     4381 tcgcagccag tctggatacg gccagtgcgg agaaaccgga gcgtacttaa gtacgtgagg
     4441 atttcgagca ctgcccggga ccaaaatggc aagtaaaata gcctgatggg ataggctcta
     4501 agtccaacga accagtgtca ccactgacac aatgaggaaa accatgtcac atctcgcaga
     4561 actggttgcc agtgcgaagg cggccattag ccaggcgtca gatgttgccg cgttagacaa
     4621 tgtgcgcgtc gaatatttgg gtaaaaaagg gcacttaacc cttcagatga cgaccctgcg
     4681 tgagctgccg ccagaagagc gtccggcagc tggtgcggtt atcaacgaag cgaaagagca
     4741 ggttcagcag gcgctgaatg cgcgtaaagc ggaactggaa agcgctgcac tgaatgcgcg
     4801 tctggcggcg gaaacgattg atgtctctct gccaggtcgt cgcattgaaa acggcggtct
     4861 gcatccggtt acccgtacca tcgaccgtat cgaaagtttc ttcggtgagc ttggctttac
     4921 cgtggcaacc gggccggaaa tcgaagacga ttatcataac ttcgatgctc tgaacattcc
     4981 tggtcaccac ccggcgcgcg ctgaccacga cactttctgg tttgacgcta cccgcctgct
     5041 gcgtacccag acctctggcg tacagatccg caccatgaaa gcccagcagc caccgattcg
     5101 tatcatcgcg cctggccgtg tttatcgtaa cgactacgac cagactcaca cgccgatgtt
     5161 ccatcagatg gaaggtctga ttgttgatac caacatcagc tttaccaacc tgaaaggcac
     5221 gctgcacgac ttcctgcgta acttctttga ggaagatttg cagattcgct tccgtccttc
     5281 ctacttcccg tttaccgaac cttctgcaga agtggatgtc atgggtaaaa acggtaaatg
     5341 gctggaagta ctgggctgcg ggatggtgca tccgaacgtg ctgcgtaacg ttggcatcga
     5401 cccggaagtt tactctggtt tcgccttcgg gatggggatg gagcgtctga ctatgttgcg
     5461 ttacggcgtc accgacctgc gttcattctt cgaaaacgat ctgcgtttcc tcaaacagtt
     5521 taaataaggc aggaatagat tatgaaattc agtgaactgt ggttacgcga atgggtgaac
     5581 ccggcgattg atagcgatgc gctggcgaat caaatcacta tggcgggcct ggaagttgac
     5641 ggtgtagaac cggttgccgg tagcttccac ggcgtggtcg ttggtgaagt ggttgagtgt
     5701 gcgcagcatc cgaacgctga caaactgcgt gtgacaaaag tgaatgtcgg cggcgatcgc
     5761 ctgctggaca tcgtctgcgg tgcgccaaac tgccgtcagg gcctgcgtgt ggcggtagcg
     5821 accattggtg ctgttctgcc gggtgatttc aaaattaaag cggcgaaact tcgtggcgaa
     5881 ccgtctgaag ggatgctgtg ctccttctct gagctgggaa tttctgacga tcataacggc
     5941 ataatcgagc tgcctgcgga tgcgccgatt ggcaccgaca tccgcgaata cctgaaactc
     6001 gatgacaaca ccatcgaaat cagcgtaacg ccaaaccgtg ccgactgttt aggtatcatt
     6061 ggtgttgcgc gtgacgttgc cgtgctgaat cagctgccgc tggttgaacc ggaaatcgtt
     6121 ccggttggtg cgaccatcga cgacacgctg ccgattacag tcgaagcgcc ggaagcctgc
     6181 ccgcgttatc ttggccgtgt ggtaaaaggc attaacgtta aagcgccaac tccgctgtgg
     6241 atgaaagaaa aactgcgtcg ttgcgggatc cgttctatcg atgcagttgt tgacgtcacc
     6301 aactatgtgc tgctcgaact gggccagccg atgcacgctt tcgataaaga tcgcattgaa
     6361 ggcggcattg tggtgcggat ggcgaaagag ggtgaaacgc tggtgctgct cgacggcact
     6421 gaagcgaagc tgaatgctga caccctggtt atcgccgacc acaacaaggc gctggcgatg
     6481 ggcggcattt ttggtggcga acactctggc gtgaatgacg aaacgcaaaa cgtcctgctg
     6541 gaatgcgctt tcttcagccc gctgtctatc accggtcgtg ctcgtcgtca tggcctgcat
     6601 actgatgcgt ctcaccgtta tgagcgtggc gttgatccgg cactgcagta caaagcgatg
     6661 gaacgtgcga cccgtctgct gatcgacatc tgcggtggtg aggctggtcc ggtaattgat
     6721 atcaccagcg aagcaacgct gccgaagcgt gcaaccatca ctttgcgtcg tagcaaactg
     6781 gatcgcctga tcggccatca tattgcggat gagcaggtaa ctgacattct gcgtcgtctc
     6841 ggctgcgaag tgaccgaagg caaagacgag tggcaggcag ttgcgccgag ctggcgtttc
     6901 gatatggaga ttgaagaaga tctggtcgaa gaagtcgcgc gtgtttacgg ctacaacaac
     6961 atcccggatg agccggtaca ggcaagcctg attatgggta ctcaccgtga agctgacctg
     7021 tcgctcaagc gcgtgaaaac gctgctcaac gacaaaggct atcaggaagt gatcacctac
     7081 agcttcgttg atccgaaagt gcagcagatg atccatccag gcgttgaagc cttactgctg
     7141 ccaagcccga tctctgttga aatgtcagca atgcgtcttt ctctgtggac cggcctgctg
     7201 gcaaccgtgg tgtacaacca gaaccgtcag cagaaccgtg tgcgcatttt cgaaagcggt
     7261 ctgcgtttcg taccagatac tcaggcaccg ttgggcattc gtcaggatct gatgttagcc
     7321 ggtgtgattt gcggtaaccg ttacgaagag cactggaacc tggcaaaaga gaccgttgat
     7381 ttctatgatt tgaaaggcga tcttgaatcc gttctcgacc tgaccggtaa actgaatgag
     7441 gttgagttcc gtgcagaagc gaatccggca ctgcatccgg ggcaatccgc agcgatttat
     7501 ctgaaaggtg aacgtattgg ttttgttggg gttgttcatc ctgaactgga acgtaaactg
     7561 gatcttaacg gtcgcactct ggtgttcgaa ctggagtgga acaagctcgc agaccgcgtg
     7621 gtgcctcagg cgcgcgagat ttctcgcttc ccggcgaacc gtcgtgacat cgcggtggtg
     7681 gtcgcagaaa acgttcccgc agcggatatt ttatccgaat gtaagaaagt tggcgtaaat
     7741 caggtagttg gcgtaaactt atttgacgtg taccgcggta agggtgttgc ggaggggtat
     7801 aagagcctcg ccataagcct gatcctgcaa gataccagcc gtacactcga agaagaggag
     7861 attgccgcta ccgtcgccaa atgtgtagag gcattaaaag agcgattcca ggcatcattg
     7921 agggattgaa cctatggcgc ttacaaaagc tgaaatgtca gaatatctgt ttgataagct
     7981 tgggcttagc aagcgggatg ccaaagaact ggttgaactg tttttcgaag agatccgtcg
     8041 cgctctggaa aacggcgaac aggtgaaact ctctggtttt ggtaacttcg atctgcgtga
     8101 taagaatcaa cgcccgggac gtaacccgaa aacgggcgag gatattccca ttacagcacg
     8161 gcgcgtggtg accttcagac ccgggcagaa gttaaaaagc cgggtcgaaa acgcttcgcc
     8221 caaagacgag taatctgatc taactaaaaa ggccgcgttg cggccttttt tcttttcact
     8281 gtcgaagagt caccgtaaaa tcaacgccat gacacttcag cagaacggat accatgctga
     8341 cacttgcccg ccaacaacag cgacaaaata ttcgctggtt attatgcctg tcagttttga
     8401 tgctgctggc gcttctctta agcctttgcg ccggtgaaca atggatctcg ccaggtgact
     8461 ggttttctcc tcgtggcgaa ctgttcgtct ggcaaattcg cctgccacgt acgctggctg
     8521 tattgctggt tggtgcggcg ctggctatat ccggcgctgt aatgcaggcg ttgtttgaaa
     8581 atcctctggc agaacctgga ctacttggcg tctctaacgg cgcaggcgtg gggcttatcg
     8641 ccgcggtatt gcttgggcaa gggcaactcc ccaactgggc gctagggctg tgtgcgattg
     8701 ctggcgcgct tatcatcact ttaatactct tacgtttcgc ccgtcgtcat ctttcgacca
     8761 gtcggttatt gctggctggc gttgcattag ggattatctg tagcgcacta atgacgtggg
     8821 ctatctactt ttccacctct gttgatttac gtcagctgat gtactggatg atgggcggtt
     8881 ttggcggcgt agactggcgg caaagctggc tgatgctggc attgatcccc gtgttgttgt
     8941 ggatctgttg tcagtccagg ccgatgaata tgttagcact tggcgagatc tcggcgcggc
     9001 aactgggttt acccctgtgg ttctggcgca atgtgctggt ggcagcgacc ggctggatgg
     9061 ttggcgtcag tgtagcgctg gcgggtgcta tcggctttat tggtctggtg atcccacata
     9121 ttcttcggtt gtgtggttta accgatcatc gcgtattact tcccggctgc gcgctggcag
     9181 gggcgagcgc attgctgctg gccgatattg tagcgcgcct ggcattagct gccgcagagc
     9241 tgcctattgg cgtggtcacc gcaacgttgg gtgcgccggt gtttatctgg ttattgttaa
     9301 aagcaggacg ttagccgcaa aaagacggtc tatgattaaa agctaagatt tttactgacc
     9361 acacccagga gaaacgatgc aagattccat tctgacgacc gtagtgaaag atatcgacgg
     9421 tgaagtgacc acgctggaga agttcgccgg taatgtgctg ttgattgtca atgtcgcctc
     9481 aaagtgtggc ttaacgccgc aatatgagca gttggagaat attcagaaag cctgggccga
     9541 tcgaggtttt gtggtgctgg gattcccgtg caaccagttt ctggaacaag aaccgggcag
     9601 cgatgaagag attaaaactt actgtaccac cacatggggg gtgacgttcc cgatgttcag
     9661 taagattgaa gttaacggcg aaggacgcca tccgctgtat caaaaattga ttgccgcagc
     9721 gccgaccgca gtcgcgccgg aagagagcgg attctatgcc cgtatggtca gcaaaggtcg
     9781 tgcaccgctg taccctgatg atattttatg gaattttgag aaatttttag ttggcaggga
     9841 cggattggtc atccagcgtt tttccccgga tatgacgccg gaagatccca ttgtgatgga
     9901 aagcattaaa ctggcgttgg caaaataatg tctattgtga tgcagttaca ggatgttgcg
     9961 gaatctaccc gcctggggcc gctttctggc gagattcggg ctggggagat cctgcacctg
    10021 gtggggccga atggcgcggg taagagtacc ttactggcgc gaatggccgg aatgaccagc
    10081 ggtaagggaa gcattcagtt cgcggggcaa ccactggaag catggtccgc aacaaaactc
    10141 gcgctgcatc gcgcctatct ttcacaacag cagacgccgc cgtttgcaat gccggtctgg
    10201 cactacctga cactgcatca gcacgataaa acgcgtaccg aactactgaa tgatgtcgca
    10261 ggggcgctgg ctcttgatga caaactcggt cgtagcacca atcaactttc cggcggtgaa
    10321 tggcaacgcg tacgtcttgc tgcggtggtg ttgcaaatca caccacaagc caatcccgca
    10381 ggccaattgc tgcttcttga tgagccgatg aacagtcttg atgttgcgca acaaagtgcg
    10441 ttagacaaaa ttctgagcgc tctgtgtcag caaggactgg cgattgtgat gagcagtcac
    10501 gatctcaacc acacgttgcg tcatgcgcat cgggcgtggt tgctaaaagg tggaaaaatg
    10561 ctggccagtg gacgcagaga agaggtgctc acgccgccaa atctggcgca ggcctatggg
    10621 atgaattttc gccgtctgga tatcgaaggt cacagaatgc tgatttcgac catctgattt
    10681 cattagtatc ttgaaaatcg ggtaattacg acgctaaatt aatacaagaa taaaaaaaca
    10741 gaggaaccgt acggaatgcg tttctgcctt attttgatca cagcactgtt tctggccggg
    10801 tgtagccacc ataaagcacc gccgccaaat gccagacttt ctgattcgat taccgttatt
    10861 gccggtttga acgaccagct acaaagctgg catggcacgc cgtatcgtta tggtggcatg
    10921 acgcggcgcg gtgtggactg ttcgggattt gtggtggtga cgatgcgcga tcgttttgat
    10981 ttgcagctgc cccgagaaac caaagaacaa gcctctatcg gcacgcaaat tgataaagac
    11041 gagttgctgc ctggtgacct ggtctttttc aaaacgggtt ccggacaaaa tggtttgcat
    11101 gtaggtattt atgataccaa caaccaattt atccacgcct ctaccagcaa gggagtgatg
    11161 cgttcctcac ttgataatgt ctattggcag aaaaatttct ggcaggcgag acgaatctag
    11221 tatgtcgaaa attggacggc gatgatacat cgccgtccat ttactccaga atacagtagt
    11281 attttgcaga caaaaattcc gtaaatggat tattcgttta taatcaggat aatctaaaaa
    11341 aaggacccct gaaaaagtgg ttaacatgga acaaatgaaa gttactcctt ctgtaattgt
    11401 tatgtcttat ggcctcatcc cttttagccg gatactgaaa aacatccttc gagagggacg
    11461 gttaccatga agattttttt ggagaatctt tatcattctg attgctattt cctgccgata
    11521 agagataacc agcaagacct tgtcggcgtt gagttaatta cccatttctc cagtgaagat
    11581 ggtacggttc gtatcccgac gagtcgggtt atagcgcaac ttaccgagga gcaacattgg
    11641 caattatttt cagagcagtt ggaattacta aaatcatgcc aacatttttt tattcaacat
    11701 aaactctttg cctggctt
//
LOCUS       DAZKFU020000142        11675 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZKFU020000142 DAZKFU020000000
VERSION     DAZKFU020000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.143"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..261
                     /locus_tag="ACHOQQ_RS15715"
                     /old_locus_tag="pgaptmp_003143"
     CDS             <1..261
                     /locus_tag="ACHOQQ_RS15715"
                     /old_locus_tag="pgaptmp_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HGU4471356.1"
                     /translation="KGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVSGH
                     GTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            265..1413
                     /locus_tag="ACHOQQ_RS15720"
                     /old_locus_tag="pgaptmp_003144"
     CDS             265..1413
                     /locus_tag="ACHOQQ_RS15720"
                     /old_locus_tag="pgaptmp_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HGU4471357.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGTLLFIIRASGY
                     VKVLNDFSIWWLALTPSGCWFFLFCLRHWQWSNQMDEHLFLKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREITDK
                     FCQLPAALPVNVTLITDQPDSEIRSAFVSAWEALFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCLGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            1406..4795
                     /locus_tag="ACHOQQ_RS15725"
                     /old_locus_tag="pgaptmp_003145"
     CDS             1406..4795
                     /locus_tag="ACHOQQ_RS15725"
                     /old_locus_tag="pgaptmp_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HGU4471358.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTPPRYVTVQDI
                     RHTLRRQYGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPVDGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTDDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPAIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVTVPESRLHGPLATTFGPVLALMDNQNNSADLLNLQTYLTRITQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLAAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNTRWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLRREGNRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPEMVFELSGTSAFTGNDEDAGDSVEETD"
     gene            4795..6393
                     /gene="tssA"
                     /locus_tag="ACHOQQ_RS15730"
                     /old_locus_tag="pgaptmp_003146"
     CDS             4795..6393
                     /gene="tssA"
                     /locus_tag="ACHOQQ_RS15730"
                     /old_locus_tag="pgaptmp_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU4471359.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKDIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADEVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLAELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNPGSS"
     gene            6395..7087
                     /locus_tag="ACHOQQ_RS15735"
                     /old_locus_tag="pgaptmp_003147"
     CDS             6395..7087
                     /locus_tag="ACHOQQ_RS15735"
                     /old_locus_tag="pgaptmp_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HGU4471360.1"
                     /translation="MSVMHYLQDKTFQDIVKNLVLPLLSGIIIPLVKWFVQHYGYAPN
                     IRKYRFEKIPVSEKESILARIDKLTKEPTTKNTLVRIKYCYEQMGIYLPIWCCNKLIC
                     FISDRNVSSVDNRLHCFLKYSFVGIFSDGKFTVNTRRVHKGYRMIAVFAVFSLCVQFT
                     GGIFTTMPFLSGGNTVLFMLFSLVYFIMIFLTVIFTCNLINEIRLAVQFGRLFEAWLK
                     SERESPEQLALF"
     gene            7095..8858
                     /gene="tssF"
                     /locus_tag="ACHOQQ_RS15740"
                     /old_locus_tag="pgaptmp_003148"
     CDS             7095..8858
                     /gene="tssF"
                     /locus_tag="ACHOQQ_RS15740"
                     /old_locus_tag="pgaptmp_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4471361.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KIAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVENVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENVSLPAGISGFDLE
                     VVLSQPWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVTGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEVDRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPVSVRNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            8813..9913
                     /gene="tssG"
                     /locus_tag="ACHOQQ_RS15745"
                     /old_locus_tag="pgaptmp_003149"
     CDS             8813..9913
                     /gene="tssG"
                     /locus_tag="ACHOQQ_RS15745"
                     /old_locus_tag="pgaptmp_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGU4471362.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYPGSRLDVRLQLCVKRSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTISLGRYQRVQEHYQRKEAQENGDYRW"
     gene            9894..10430
                     /gene="tssJ"
                     /locus_tag="ACHOQQ_RS15750"
                     /old_locus_tag="pgaptmp_003150"
     CDS             9894..10430
                     /gene="tssJ"
                     /locus_tag="ACHOQQ_RS15750"
                     /old_locus_tag="pgaptmp_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU4471363.1"
                     /translation="MAIIAGKTGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWLQPGGSVAVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            10433..10882
                     /gene="tssE"
                     /locus_tag="ACHOQQ_RS15755"
                     /old_locus_tag="pgaptmp_003151"
     CDS             10433..10882
                     /gene="tssE"
                     /locus_tag="ACHOQQ_RS15755"
                     /old_locus_tag="pgaptmp_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGU4471364.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSEEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTSILQGMPGTAHQLMCVLSDVLLKYEPRIKRVDVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            10935..11240
                     /locus_tag="ACHOQQ_RS15760"
                     /old_locus_tag="pgaptmp_003152"
     CDS             10935..11240
                     /locus_tag="ACHOQQ_RS15760"
                     /old_locus_tag="pgaptmp_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471365.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            11295..>11675
                     /locus_tag="ACHOQQ_RS15765"
                     /old_locus_tag="pgaptmp_003153"
     CDS             11295..>11675
                     /locus_tag="ACHOQQ_RS15765"
                     /old_locus_tag="pgaptmp_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HGU4471366.1"
                     /translation="MTSEVKLKTGNDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTVSWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDN"
BASE COUNT         2653 a         2735 c         3323 g         2964 t
ORIGIN      
        1 aaaggttttg tattgcttgg tgataatacc acacatggtg gtaaagttat ttcggcatct
       61 tcaaccatga ttgtcgatgg taaaaaagta gctcttgttg gagatatggt tagttgccct
      121 gtttccggac atggtaccaa tcctattgtg gagggctctc ctgaatggat atctgatgga
      181 agagctgttg tgattgatgg atgtaaatgt ctgtgtgggt gtaaggttat atccagtgca
      241 ccggaatgtg caataggata aaccatggga tggagcagaa caaaatcttt atcaacagaa
      301 ttacctcata aaccatcaat gatgttgtgg tttatgacag gggcatttat ggctgttgtt
      361 ggtactctgt tgtttattat ccgggcatca ggatatgtga aagtattaaa tgatttcagt
      421 atctggtggc ttgcattgac tccttcgggg tgctggtttt tcctgttttg cctgcgacac
      481 tggcagtgga gcaaccagat ggatgaacat ttgtttctta aaaaagaggg ggagtatgct
      541 cagaaacagt gggaatcctg ggccgaaagg tatcttgtta ttactgccag ttgtgtttat
      601 ttgcctgata aaataacggt agctactctt tgtgatgaat tgcctctgca gtacggcctt
      661 gtaaagaaaa tagattatct ttctgacagt gggcataagg ttgaagcctc tttacgtgtc
      721 ttgctaaggg agataacgga taaattttgt cagttacctg ccgctctccc tgtaaatgtg
      781 acgctgataa ccgatcagcc tgattctgaa atcagatctg cttttgtttc agcatgggaa
      841 gcgttatttc cccaacgcgt cgttcctgac aatatcgagg ttactcccga cttttccatg
      901 gggtgggttg atgagcgtct gaaacaacca gtgctgactg tggatttgat actggtaata
      961 cagcttaatg ggggaaatgc atactctgac ggactggctg cacttttgtt gaccagcgat
     1021 gatgttgcgc aaaaatataa cctgccgcat ccagcccgtc tgctacgccc gatgtcgctg
     1081 gatatcaaca agtttaatga tgaattcaca cttttccttg aaacccagac agctgcctgt
     1141 cgtaccgctc gtgtactggg ggactgttat cactgggaaa agattgctgc accgctgatg
     1201 actattggaa accagtatgg tgccggatgg gaaccgtccg gacgtatgtt actggaaaaa
     1261 tggtgcggta tcccgggacc tgctgctccg tggctgctta ctgctctggc agccgatctg
     1321 gtctgtcttg gtaatgattc tctcctgact ttattctcat ccggagaaga acattttatc
     1381 agtactgtta catcaggaaa ccagaatgaa taaactggcc tgtctgtctg gtcgctttgg
     1441 acgaccgggg attgtattta ttggtgtggc tgcgctctgg tggctgataa cccgatatgg
     1501 agcatttctg ggggcagaaa cacgccggga tcaaatacta ctgctgatat tgctgagtct
     1561 ggggttgctc tttgtctgtt acctgcctgt gatgaaaaaa tatgttcagg aactgacata
     1621 tcgacgtcgg gcccggaagg agcaaaggtt acctgacgat gaagagcgac tggctcagac
     1681 gccaccgcgc tatgttactg ttcaggatat tcgtcatact ctgcggcgcc agtatggcag
     1741 attctgggga cgtaaaatac gcattctgtt gataacaggt actgcctctg aggttgagtt
     1801 gctgaccccg ggcctgacag aacagttctg gcaggaagag caggggacat tgcttctctg
     1861 gggaggagac ccctcgcagc cggaaaacgc agactggctg gctgcgttac gccgtctgcg
     1921 ttaccggcct gttgatggca ttgtctgggt aacttccgga ctgagtgaaa cattatccgc
     1981 accactgaca gaagatgccc tggacagggt ttcccgtgcg gtcagttcat gttgtgagcg
     2041 ccttggctgg cgtctgccgc tgtatgtctg gtcactgcag gagagtccgg atgagcgagg
     2101 gcgtattact cagcccgtgg gatgccttct gccggcagaa tgtagttctg acaaactgaa
     2161 agcacaacta caggccatgc tccccgggct tgttgctcag gggatacagc agatatgctg
     2221 tgcccctcgg tattattttt tgttgtcgct ggcagagcga ttcagacgaa atattgatgc
     2281 tgtggtggag ccattatccg tattactccg tccataccgt cagttgttgc tggcgggtat
     2341 tgttttcagt ccggcaactg tcggcggtga acgaagtgtc agacatcgct ggcgaatgga
     2401 taaccgctgg gaggcgttgc cggagacagt gcagcaatta cctgtccggc tacagccttc
     2461 ccgcacaggt cataactggc gcagaagtct ggccgttatg gcggccatac tgatgatggc
     2521 tcagggaaca ggaatggtgg tttcctttct ggcaaaccgg agtctggttg ctgaagtgca
     2581 ggaacagatt cgtccggctc agaaccagca actgtctcct gcagaacgtc tgcaggcgtt
     2641 gctgaatctg cagaaatccc tggcccgcct tcagtaccgg gaggagcacg gggcgccatg
     2701 gtacctgcgg gccggtatga atcagaatgc agacctgctc gcggtcgtaa tgccccttta
     2761 tgcacaaaat gcacatcttt tactgcgtga tgccgctgct gctcatcttg agcagcagct
     2821 caggacattt attcgtcttc ctccggacag cccgcagcgg gggaaaatgg caaaagccgc
     2881 atatgaccag ctcagactgt atctgatgct ggcgcaaccg caacatatgg agcccgcctg
     2941 gttcagtcgt acgctcatgc gggaatggcc acagcgtgac ggtgtgagtg ccgttttctg
     3001 gcaggctaat ggcccgacgt tgctggcgta ttatgcctcc gggattatta ctcatcctca
     3061 gtggaagctg acggctgatg aagagctggt cagccagagc agaactctgc tgcttcgcca
     3121 tctggggaca cagaacagcg atgccatgct gtatcagaaa atgctggcac gggtggcaca
     3181 tcagtttgcg gatatgcgtc tgacggatat gaccgatgat actgatgtct cccgcctctt
     3241 ttttacggat gaagtggttc ccggtatgtt cacccgccag gcctgggaag aggccgtatt
     3301 gccggcgata gacaccgtga ttaatgagcg tcgtgaagaa atggactggg tactgacgga
     3361 tggcaggcag aaggcaccgt caccggtatc gccggaagca ctcagacagc gcctgacaac
     3421 gcgctatttt gctgattttg gtaatgcctg gctgaatttt ctgaacagtc tgcacttgcg
     3481 taaagctcag acgctgtctg atgtgacaga gcaactgacg ctgatggctg atgtgcgcca
     3541 gtcccccctg gttgcactga tgaatacgct ggcggttcag gggcgtactg gtcagccccg
     3601 ggaagcggtt actgattcac tggttaaatc tgccagaaac ctgctttcac aggaaaaaca
     3661 gccagtgaca gtaccggaaa gccggcttca cggtcctctg gcgacgactt tcgggccggt
     3721 gcttgcactg atggataatc agaacaacag cgcggatctg ctgaatctgc agacgtatct
     3781 gacccggatt acacaggttc gtctgcgtct gcaacagatt gccggctctt ctgacccgca
     3841 ggccatgatg cagatgctgg cacagacagt attgcagggg aaatcggttg atttaactga
     3901 tacccgggat tacggcagcc tggccgctgc cggactcgga caggagtggt atggcttcgg
     3961 acagacggtc tttgttcgcc cgatggagca ggcgtggcag caggttctta cacccgcggc
     4021 agaaagtctg aatacccggt ggcgtactgc ggtggtggat ggctggaata atgcattttc
     4081 cgggcgttat ccgtttaaaa atgtcagcag cgatgcttca cttcccctgc tggcaaaata
     4141 cctgaatacg gacacgggac gtattgcccg ttttctgcag aataacctga gtggtgtgtt
     4201 gcgcagggaa ggcaatcgct gggtaccgga taccattaat acccgggggc tgacattcaa
     4261 ccctgcattt ctgaaagcca tcaataccct gagtgaaata gcagatgtgg cgttcaccac
     4321 aggtaacgcg gggctgcatt ttgagctgcg cccgggaact gctgccggtg tgatgcagac
     4381 gacgctgata accgataatc agaaactgat ttatgttaat cagatgccgg tatggaagcg
     4441 atttacctgg ccggctgata cggaagcacc tggcgccagt ttaagctggg taagcactca
     4501 ggccggtacc cgtcagtatg cagacctgcc gggaagctgg gggctgatac gtctgcttga
     4561 gatggcccga cggaaagccg caccgggcgt tgccagtggc tggagcctga gctggcaggc
     4621 gcaggacggt cgtatgctga attacacact gcgaactgaa gcgggggaag ggccgcttgt
     4681 attgctgaaa ctccgcaatt ttgttctgcc ggaaatggtt ttcgaactct ccggtacgtc
     4741 agcgtttaca ggcaatgacg aagatgccgg ggattccgtg gaggagactg actgatggct
     4801 tccattcatt cgctcctcag tgcatgccag acgacacccc gggatgtggc tgaaccggct
     4861 caggtgcgta ttgcgctgtg ggataaatgg cttgctcccg tgacgccaga taatcccgca
     4921 ggtgatgatg cggggtacga tgatgatttt cagcagatgc gtgaagaggt caataaactc
     4981 tccggagccg atgccggaat tgtcagccag cttgcagaaa aattactgac caccagaacc
     5041 aaagatatcc gggtggcgac ctggtatatc tgggcccgtc tgcggcagga tggtgaaaaa
     5101 ggtctggccg acggtctgga gctgctgacc ggtttgttgc agcgttttgg agagcacctg
     5161 catccacaac ggtcacgggc caggaaggcg gcgctggagt ggttatgcag cgcccgcata
     5221 ctggacagtc tgtctctgta tccggaggtg gtgaaggcgg ataccctgcg cattgccggg
     5281 gcgctgtggc tggcagaaca gacctttacg gatgaggctt ccgccccggt gctgaacggg
     5341 ttatatcagg cacttgaaaa tcgcctgatg aaggccggtg gtgttgatgc agtggtgcct
     5401 caggaggctg ccgcgccggc accgacggta acatccggca gcgtcatggc attatctgcc
     5461 atcacctccg gacaggaact gctgtcacag gcccgtgttc tggcaaaata tctgcgtgac
     5521 cagcctgagg gctggctggc ggctcatcga ctgatgaaaa gcgtccggca tgacacgctg
     5581 catcagttac cgccgttatc tgctgatgga cggacccgta ttgccccacc ggggccggat
     5641 cggcgtgcgt cactgaaacg gttgtatctg cagcagaact ggttgtcact gcttgaacag
     5701 tgtgacgata tgtttgcccg gggagccagt catctgtggc tggacctgca gtggtatatt
     5761 catcaggcgc tgttgcagac cggaaaagaa aattatgctg ccattattca gtatgacctg
     5821 aaaggattgc ttctgcgcct gccggggctt gagacactgg cttttaacga cggcatgccg
     5881 tttgccgatg aagtgacgct aagctggata cagcagcagg tgatggagtg tggtgaacgc
     5941 tgggcggaag aaccgtctgt aacgataacg gcagcccccg gagataacga cattttatct
     6001 cttgagccgg aagccctgca gattgcggat aatgaaggaa cagaagccgc tttaagctgg
     6061 ttacaggcac gtcccggtat tcagtctgac cgcagcaact ggctactgcg cctgttgatg
     6121 gcccgtgttg ccgaacagac tggaaaaaat gaccttgccc tgcatctgct tgcggagctg
     6181 gatgaacgtg caacccggct gaccctgtca caatgggaac ctgaactggt atttgaggta
     6241 aaagcccgcc gcctgaaact gttacgtatg aagtccgcaa aaactgaaag tgacagggtg
     6301 cgtctgcagc ctgatatgga acatcttctt gccgggctca tcgccattga tgctgccaga
     6361 gctgctgtgt tgtgcaatcc ggggagctca tgagatgtct gtcatgcatt atcttcagga
     6421 caaaacattt caggatatag ttaaaaatct ggtgttaccg cttctgtcgg gcattattat
     6481 tcctcttgtc aaatggtttg tgcagcatta tggctatgcg ccgaatatca ggaaatacag
     6541 atttgagaaa attcctgtat ctgaaaagga gtccattctt gcccgtattg acaaactgac
     6601 aaaagagcca acaacgaaaa atacactggt aagaataaaa tactgttatg aacagatggg
     6661 gatatatctt ccgatatggt gttgtaataa gctgatttgt tttatatccg acagaaatgt
     6721 cagttcagtg gataacagac tgcattgttt tcttaaatat tcttttgtcg gtatattttc
     6781 agatggcaaa tttactgtta atactcgtcg tgtgcataaa ggttaccgga tgatagcagt
     6841 gtttgcggtg ttttctttat gtgttcagtt tacgggaggg atatttacaa ccatgccatt
     6901 tctgtcgggg ggtaatacag ttttatttat gttattttcc ctcgtttatt ttatcatgat
     6961 atttctgaca gttatattca catgtaatct gattaatgaa atccggcttg ccgttcagtt
     7021 cgggcgattg tttgaggcat ggctgaagtc agagcgcgag tctccggaac agttagcttt
     7081 attctgagaa gataatggac gatttaaccc tgcgttattt tgacgctgaa atgcgttatc
     7141 tgcgtgaagc cggaaaggcg tttgcacagg ctcatcccga tcgggcagcg atgctggatc
     7201 tcgataaagc gggtacgcct gatccctgcg tggaacgcct gtttgaaggt tttgcctttt
     7261 ccatgggacg actgcgccag aaaatcgacg atgatttgcc ggagcttact gaaagtctgg
     7321 tcagtatgct ctggcctcat tacctgcgta ctatcccgtc cctgtcggtg gtggcactga
     7381 ccccccggtt atccgtcatg aaaatagctg aaaccgtacc agccggactg gaagtgactt
     7441 cccgccctgt tggccccgga aatacagtct gtcgttaccg taccacccgg gccataccac
     7501 tgaatccact ggcagtggag aatgtcgtta tgacgacgga gccggacggg cgctcagtgc
     7561 tgaaaatagg ttttgcctgc agtgaactgg ctgactggtc tcaggtcgat ttgcatcgtc
     7621 tgagtctgta tctggcagca gaagccccgg tcagcagcac ccttcatctg atgatgacaa
     7681 agcgtctggc tgcgctgtat ctgaggctgc cggggaatga tgagcggatc cgcattgacg
     7741 gatggttttc acccggcggt tttgcagaag aagatcggct gtggccgaaa ggtgacagtg
     7801 ctttcagtgg gtatcagtta ttgctggaat atttcacctt tcgggaaaag tttatgtttg
     7861 ttcatctgaa cggactggag aatgtttctc tgccggcagg tatttccgga tttgatcttg
     7921 aggtggtgct gagtcagccc tggccggcag atttacctgt gacagacgat gccctgtgtc
     7981 tgcattgtgt gccggtgatt aaccttttta cgcttgaagc cgatccactc atcatcaatg
     8041 ggctggagag cgaatacctg ctgcgcccga agcgtctgca ggatgggtat acggagatat
     8101 attcagtgga tgcggtgacg ggctccgggc gaaccggcag tgcagagtac gtgcctttta
     8161 ccagcttccg tcaccgtggg ggaatgctgc gccatgatgc acccgaacgt tattatcata
     8221 cccgggtgaa gcggggtgtc acggggatgt atgacacatg gcttatcctt ggcggtcagc
     8281 ggtgggaggt ggatcggatg ccggagcggg aaacgctttc attacgtatt acaggtacca
     8341 atggtcagct tccccgtcgt gcgttgcaga gtacgctgct tgatcggtgt gaacaggtac
     8401 tgcagacccc ggtcagtgta cgtaatcttt gtaaacccac gctaccggtt tatccgccga
     8461 cagaagatcg ttttcactgg cgggtgatga gtcatctggg gacaggattt cttaacatgc
     8521 tgagcagtgc ggaagtgctg cgtggcacgc tggcgcttta taactggcgg gacgatgaac
     8581 ttaatcaccg tcggcttgat gccattctgg ctgtacagca tcatcgtatt cagcgttttg
     8641 aaaagggttt tctgctccgg gggcttgatg tggaggtaac tcttgatggc aacgggtttg
     8701 ccggagaggg agatattcac ctgtttggcg aaatgctcaa ccgtttcctg gccctgtacg
     8761 ccgatatgaa tcagttcaat cagctcacac tgattgttca gcctgaagga aaatgcatcc
     8821 ggtggaaaga aaatcacaat ccgcgcctgc ccggttaatt acccgttacc ggaaacagct
     8881 tccgtatatt aatttttatc gcttctgtca gttactggaa caaagccagc cagatcagcc
     8941 tcccattggc agtggctggc aggcccgtca ggaagcggtt cgcttttgtc catacacggg
     9001 aatggggttt cctgcgagtg aaataaaaga tgctgtaatt cctgaagaat cccatctgcc
     9061 acccactgta catgtgacgt ttatgggact ttacggggtc acgtctccgt tgccggctca
     9121 ctatatcagt gatattgcac agcagcggga aggccatgaa gccgcagcgg attttctcga
     9181 tatattcagt catcgtctga tcacccagta ttaccgtatc tggcgcaaat attcgtaccc
     9241 ggccaccttt gaggcggggg ggcaggataa aacatcccag tatctgctcg gtctggccag
     9301 actggggatc cccggatgtg cacagaatat tgccacgccg gtatcgcgtt ttctggcgtt
     9361 attgcccctg atgttgttac ccggaagaac agctgaaggg ctgacatcac tggtaacgct
     9421 gctggctccc ggaacacagg cccgtgtctg gcatcatgac cgacgacgga tcccgctgaa
     9481 aacaccgctg gccatgcgtg ttcatcatcc ggtcagtctg aagagtcgcc cggtgatggg
     9541 cgatcatgct acggatgtga acggacaggt gttattacag ctttcaacac agacgggcag
     9601 cgaggtacag ggctggttac cgggcgggca gctgtattct gatttgctgg cgttgttgca
     9661 cgtttatccg ggaagccgtc tggatgtacg tctgcaactt tgtgttaagc gttcactgtt
     9721 gccggatgtc cggctcagtt gccgaccggc agccggtagt ccgcaactgg ggcgcacggc
     9781 tgtaatgaga acacaggcaa agataacgac ttcagcggca cgcgtaatga ccatcagtct
     9841 ggggcgttat cagcgggttc aggaacatta tcaacgtaag gaggctcagg aaaatggcga
     9901 ttatcgctgg taagactggt tacggtctga ttattgcgtt gttttccctg tcgttatcag
     9961 ggtgtggcct gacgcaaaga gtggcagacg gtacggtatc tgccacgaaa tccctgtttt
    10021 accgacagat aaaaacactt caccttgata ttcgtgccag ggaggccatt aacaccagtg
    10081 ctgccggtat tcccctgtcg gttgtggtgc ggatttatca gcttaaagac aaccggaatt
    10141 ttgacagcgc ggattatcag gcgcttttta cgggggacag tgaaattctg gcaggcgata
    10201 tcattgcaca gaaagatgta tggctgcagc caggtggttc agtggccgtg gatatgcctc
    10261 tggatgatgc ggcgaaattt accggtgtgg cagcaatgtt tctggagcct gaccggaaga
    10321 aaaatacctg gcgggtggtg ctgagccggg atgagcttga gccggacacg ccccgtctta
    10381 ttgaagtatc cggtaacacc ctgacgctgt taccggtgaa ggataaatag tgatgccgcg
    10441 tccttccctt tatgaaattc tctatggcaa tttcgctggc gggctggagt taaaccaggt
    10501 cagtgaggaa gagcaggtta ttctttcggt gctcgataac atgcagcgta ttctgaatac
    10561 ccgggccgga agccttaaac atcttccgga ttatggtctg ccggatatga caagcattct
    10621 gcagggaatg ccgggaacgg cgcatcaact gatgtgtgtg ctttcagatg ttctgctgaa
    10681 gtatgagcca cgtattaagc gtgttgacgt gaccatgcag gagcagactc agcctggtga
    10741 gttgcattat gtcattgatg cagaacttaa agacgcagga ctggttcgtt acggaacaac
    10801 atttatgccg gagggcaggg tgttactgcg atatctgaag caacagcagt ttgtggataa
    10861 tgtgatgact ttcagggggt gatttatggt tccccttatc cgtgtgctta acagacataa
    10921 acgagggctg aatgatgaac ccgttattta atgacatcca gatgcgcctt ttttatctga
    10981 atcatgctcc gtattcctgg cactggaatg tcagattcag gccacaggaa gccgtatata
    11041 ttggtaatga tgcctgccat attaccatta cctgtaatca gtccggtttt catttaacca
    11101 gggacgggca gcgactgttc actgaacgtt atatcagaac actcagtgag ttattggcgg
    11161 ttctgaagcg gcggtgggat gtgacaccgg caatcatccg tgcggtggaa tacctttccc
    11221 gggtaccggt attacactga cgctctttcc gggaatatta attatgattt gatacagcaa
    11281 cactgaatct gccgatgact tctgaagtaa aactgaaaac aggtaatgac ccccggtcat
    11341 taccggatta tgctgccctg cgcgatgaaa tcagcaagct gactcacccg gcacgtccgg
    11401 atgtggactg gcgttatgtg gagacgctct gcctgcgtct gtatgaacac aatggcgtgg
    11461 aattacagac ggtatcctgg tacaccatcg cccggatgca tacagcaggg ttaagtggtc
    11521 tgaatgaagg gctggctctg attgtggcgc tgacccggca tcactggtca gtgatgtggc
    11581 cgctgaatac ccacgcccgg ctggaaatta ttaccggtct gttcagtcgt ctgcaaaaaa
    11641 cgctccgggc catgccaccg gatgagcgtg acaat
//
LOCUS       DAZKFU020000143        11566 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZKFU020000143 DAZKFU020000000
VERSION     DAZKFU020000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.144"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..184
                     /locus_tag="ACHOQQ_RS15770"
                     /old_locus_tag="pgaptmp_003154"
     CDS             <1..184
                     /locus_tag="ACHOQQ_RS15770"
                     /old_locus_tag="pgaptmp_003154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471367.1"
                     /translation="LCRISEDPHHWNGFSGEKRILYGHSAHHFFFSSSTIHVSLPRLI
                     TDYEVQKISFFSVACH"
     gene            225..2648
                     /gene="pgaA"
                     /locus_tag="ACHOQQ_RS15775"
                     /old_locus_tag="pgaptmp_003155"
     CDS             225..2648
                     /gene="pgaA"
                     /locus_tag="ACHOQQ_RS15775"
                     /old_locus_tag="pgaptmp_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HGU4471368.1"
                     /translation="MYSSSRKRCPQTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKEHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQTRGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2657..4675
                     /gene="pgaB"
                     /locus_tag="ACHOQQ_RS15780"
                     /old_locus_tag="pgaptmp_003156"
     CDS             2657..4675
                     /gene="pgaB"
                     /locus_tag="ACHOQQ_RS15780"
                     /old_locus_tag="pgaptmp_003156"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HGU4471369.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSKTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFRWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSPLIEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     AGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGLLFHDDALLSDYEDASAPAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVTQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLINQIKSIPQAKDKSILELQAKNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4668..5993
                     /gene="pgaC"
                     /locus_tag="ACHOQQ_RS15785"
                     /old_locus_tag="pgaptmp_003157"
     CDS             4668..5993
                     /gene="pgaC"
                     /locus_tag="ACHOQQ_RS15785"
                     /old_locus_tag="pgaptmp_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HGU4471370.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5995..6408
                     /gene="pgaD"
                     /locus_tag="ACHOQQ_RS15790"
                     /old_locus_tag="pgaptmp_003158"
     CDS             5995..6408
                     /gene="pgaD"
                     /locus_tag="ACHOQQ_RS15790"
                     /old_locus_tag="pgaptmp_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HGU4471371.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6458..7246)
                     /gene="phoH"
                     /locus_tag="ACHOQQ_RS15795"
                     /old_locus_tag="pgaptmp_003159"
     CDS             complement(6458..7246)
                     /gene="phoH"
                     /locus_tag="ACHOQQ_RS15795"
                     /old_locus_tag="pgaptmp_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGU4471372.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7878..9149)
                     /gene="efeB"
                     /locus_tag="ACHOQQ_RS15800"
                     /old_locus_tag="pgaptmp_003160"
     CDS             complement(7878..9149)
                     /gene="efeB"
                     /locus_tag="ACHOQQ_RS15800"
                     /old_locus_tag="pgaptmp_003160"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGU4471373.1"
                     /translation="MQYEDENGVNELSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLVKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9155..10282)
                     /gene="efeO"
                     /locus_tag="ACHOQQ_RS15805"
                     /old_locus_tag="pgaptmp_003161"
     CDS             complement(9155..10282)
                     /gene="efeO"
                     /locus_tag="ACHOQQ_RS15805"
                     /old_locus_tag="pgaptmp_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGU4471374.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTEADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10340..11170)
                     /gene="efeU"
                     /locus_tag="ACHOQQ_RS15810"
                     /old_locus_tag="pgaptmp_003162"
     CDS             complement(10340..11170)
                     /gene="efeU"
                     /locus_tag="ACHOQQ_RS15810"
                     /old_locus_tag="pgaptmp_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGU4471375.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3034 a         2844 c         2730 g         2958 t
ORIGIN      
        1 tttatgccgt atttctgaag atcctcatca ttggaatgga ttttcgggcg agaaaaggat
       61 tttatatgga cactctgctc atcatttctt cttctcatca tcaacaattc acgtctctct
      121 tccgcgttta ataacggatt atgaggtgca aaaaatatct ttcttttcag ttgcctgtca
      181 ttagatacag agagagattt tggcaataca tggagtaata caggatgtat tcaagtagca
      241 gaaaaaggtg cccgcaaacc aaatgggctt tgaaaattct tactgccgca tttttagcag
      301 cgagtcccgc ggcgaagagt gccgttaata acgcctatga tgcattgatt attgaagctc
      361 gcaagggaaa tactcagcca gctttgttat ggtttgcaca aaaatcagca ctcagtaata
      421 accaaattgc tgactggtta cagattgcct tatgggccgg gcaagataaa caggttatta
      481 ccgtttacaa ccgctaccgt tatcagcaat taccagcgcg tggttatgca gctgtcgccg
      541 tcgcttatcg taacctgcaa caatggcaaa actcgcttac actgtggcaa aaggcgctct
      601 ctctggagcc gcaaaataag gattatcaac ggggacaaat tttaaccctg gcagatgcgg
      661 gtcactatga tactgcgctg gttaaactta agcagcttaa ctctggagca ccggacaaag
      721 ccaatttact cgcagaagcc tatatctata aactggcagg gcgtcatcag gatgaattac
      781 gggcgatgac agagtcatta cctgaaaatg cctctacgca acaatatccc acagaatacg
      841 tgcaggcatt acgtaataat caacttgctg ccgcgattga cgatgccaat ttaacgccag
      901 atattcgcgc tgatattcat gccgaactgg tcagactgtc gtttatgcct acgcgcagtg
      961 aaagtgaacg ttatgccatt gccgatcgcg ccctcgccca atacgctgca ttagaaattc
     1021 tgtggcacga taacccagac cgcactgccc agtaccagcg tattcaggtt gatcatcttg
     1081 gcgcgttatt aactcgcgat cgttataaag acgttatttc tcactatcag cgtttaaaaa
     1141 agacggggca aattattccg ccctgggcgc aatattgggt tgcgtcggct tatctcaaag
     1201 aacatcagcc aaaaaaagca cagtcaataa tgacagagct cttttatcac aaggagacca
     1261 ttgccccgga tttatccgat gaagaacttg cggatctctt ttacagccac ctggagagtg
     1321 aaaattatcc cggcgcgcta actgtcaccc aacataccat taatacttcg ccgcctttcc
     1381 ttcggttaat gggcacgcct acgagcatcc cgaatgatac ctggttacag gggcattcgt
     1441 ttctctcaac cgtagcaaaa tatagtaatg atcttcctca ggctgaaatg acagccagag
     1501 agcttgctta taacgcacca ggaaatcagg gactgcgcat tgattacgcg agtgtgttac
     1561 aaacccgcgg ttggcctcgt gcagcagaaa atgaattaaa aaaagcagaa gtgatcgagc
     1621 cacgtaatat taatctggag gttgaacaag cctggacagc attaacgtta caagaatggc
     1681 agcaggcagc tgtcttaacg cacgatgttg tcgaacgtga accgcaagat cccggcgttg
     1741 tacgtttaaa acgtgcggtt gatgtacata atcttgcaga gcttcgtatc gctggctcaa
     1801 caggaattga tgccgaaggc ccggatagtg gtaaacatga tgtcgactta accaccatcg
     1861 tttattcacc accgctaaat gataactggc gcggttttgc tggattcggt tatgccgatg
     1921 gacaatttag cgaaggaaaa gggattgttc gcgactggct tgcgggtgtt gagtggcggt
     1981 cacgtaatat ctggctcgag gcggagtacg ctgaacgctt tttcaatcat gagcataaac
     2041 ccggcgcgcg cctgtctggc tggtatgatt ttaatgataa ttggcgtatt ggttcgcaac
     2101 tggaacgcct ctctcaccgc gttccattac gggcaatgaa aaatggtgtt acaggcaaca
     2161 gtgctcaggc ttatgttcgc tggtatcaaa atgagcggcg taagtacggt gtctcctggg
     2221 ctttcactga tttttccgac agtaaccagc gtcatgaagt ctcaattgag ggtcaggaac
     2281 gcatctggtc ttcaccatat ttgattgtcg atttcctacc cagtctgtat tacgaacaaa
     2341 atacagaaca cgatacccca tactacaacc ctataaaaac gttcgatatt gttccggcat
     2401 ttgaggcaag tcatttgtta tggcgaagct atgaaaatag ctgggagcaa atcttcagcg
     2461 caggtgttgg tgcctcctgg caaaaacatt atggcacgga tgtcgtcacc caactcggct
     2521 acgggcaacg cattagctgg aatgacgtga ttgatgctgg cgcaacgcta cgctgggaaa
     2581 aacgacctta tgacggtgac agagaacaca acttatacgt tgaattcgat atgacattca
     2641 gattttaagg ataaatatgt tacgtaatgg aaataaatat ctcctgatgc tggtgagtat
     2701 aattatgctc actgcgtgca ttagccagtc aaaaacatca tttataccgc cacaggatcg
     2761 cgagtcttta ctcgccgagc aaccgtggcc gcataatggt tttgtggcga tctcatggca
     2821 taacgttgaa gacgaagctg ccgaccagcg ttttatgtca gtgcggacat cagcactgcg
     2881 tgaacaattt gcctggctgc gcgagaacgg ttatcaaccg gtcagtattg ctcaaattcg
     2941 tgaagcacat cgaggaggaa aaccgctacc ggaaaaagca gtagtgctga cttttgatga
     3001 cggctaccag agtttttata cccgcgtctt cccaattctt caggccttcc ggtggccagc
     3061 tgtatgggcc cccgtcggca gttgggtcga tacgccagcg gatgaacaag taaaatttgg
     3121 cgatgagatg gtcgatcgag aatattttgc cacgtggcaa caagtgcgag aagttgcgcg
     3181 ttccccgctc attgaggtcg cttctcatac atggaattct cactacggta ttcaggctaa
     3241 tgccaccggc agcttattgc ctgtatatgt aaatcgtgca tattttactg accacgcacg
     3301 gtatgaaacc gcagcagaat accgggaaag aattcgtctg gatgctgtaa aaatgacgga
     3361 atacctgcgt acaaaggcta aggtaaatcc acacgttttt gtttggcctt atggcgaagc
     3421 gaatggcata gcgatagagg aattaaaaaa actcggttat gacatgttct tcacccttga
     3481 atcaggtttg gcaaatgcgt cgcaattgga ttccattccg cgagtattaa tcgccaataa
     3541 tccctcatta aaagagtttg cccagaaaat tattaccgta caggaaaaat caccacaacg
     3601 gataatgcat atcgatcttg attacgttta tgacgaaaac cgccagcaaa tggatcgcaa
     3661 tattgatgtg ctaattcagc gggtgaaaga tatgcaaata tcaaccgtgt atttgcaggc
     3721 atttgctgat cccgatggtg atgggctggt caaagaggtc tggtttccaa atcgtttgct
     3781 accaatgaaa gcagatattt ttagtcgggt tgcctggcaa ttacgtaccc gcgcaggtgt
     3841 aaacatctat gcgtggatgc cggtattaag ctgggattta gatcccacat taacgcgagt
     3901 aaaatactta ccaactgggg agaaaaaagc acaaattcat cctgaacaat atcgccgtct
     3961 ctcgcctttt gatgacaggg tcagagcaca agttggcatg ttatatgaag atcttgccgg
     4021 acatgctgct tttgatggcc tattgttcca cgatgatgct ttgctttcag attatgaaga
     4081 tgccagtgca ccggctatcg cggcttatca gcaagcaggc tttagcggga gtctgagcga
     4141 aattcgacaa aacccggagc aatttaaaca gtggacccgc tttaaaagtc gtgcgttaac
     4201 tgacttcact ttagaactta gtgcgcgcgt aaaagccatt cgtggtccac atgttaaaac
     4261 tgcacgaaat attttcgcac ttccggtaac acaacctgaa agtgaagcct ggtttgcaca
     4321 gaattatgct gatttcctaa aaagctatga ctggactgct attatggcta tgccttatat
     4381 ggaaggtgtc gcagaaaaat cggctgatca atggttaata caactgatca atcagattaa
     4441 aagcatccct caggctaaag acaaatctat tttagaatta caggcaaaaa actggcagaa
     4501 aaatggtcag catcaggcta tttcttcgca acaactcgct cactggatga gcctattaca
     4561 actgaatgga gtgaaaaact atggttatta tcccgacaat tttctgcata accaacctga
     4621 aatagacgtt attcgtcctg agttttcaac agcctggtat ccgaaaaatg attaaccgca
     4681 tcgtatcgtt ttttatatta tgtctggtgt tatgcatccc cctatgcgta gcgtactttc
     4741 actctggtga actgatgatg aggttcgttt tcttctggcc gttttttatg tccatcatgt
     4801 ggattgttgg cggcgtctat ttctgggtct atcgtgaacg ccactggccg tggggggaaa
     4861 acgcaccagc tccccagttg aaagataatc cgtctatctc cattatcatt ccctgtttta
     4921 atgaggagaa aaacgttgag gaaaccatac acgccgcttt agcacagcgt tatgagaaca
     4981 ttgaagttat tgccgtaaat gacggttcaa cagataaaac ccgtgccatc ctggatcgca
     5041 tggctgcaca aattccccat ttgcgggtca ttcatctggc gcaaaaccag gggaaagcca
     5101 ttgcgcttaa aaccggagct gccgcggcga aaagtgaata tctggtgtgc attgatggcg
     5161 acgcgttatt agaccgcgat gcggcggcat atattgtgga accgatgttg tacaacccgc
     5221 gtgtgggtgc cgtaaccggt aatcctcgta ttcgcacacg ttctaccctg gtgggtaaaa
     5281 ttcaggttgg cgagtattcc tcaattattg gtttgatcaa gcgaacccag cgtatctatg
     5341 gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg tcgcagcgcc ctggcagaag
     5401 tgggttactg gagtgacgat atgatcaccg aagatattga tattagctgg aagctgcagt
     5461 tgaatcagtg gacgattttt tacgagccac gggcgctgtg ctggatatta atgcctgaaa
     5521 cgttaaaagg gctgtggaaa cagcgcctgc gctgggctca gggcggtgca gaagtattcc
     5581 tcaaaaatat gacaaggttg tggcgcaaag aaaactttcg aatgtggcca ctgttttttg
     5641 aatactgcct gacgacaata tgggccttta cctgcctggt cggtttcatt atttacgcag
     5701 tccaacttgc cggtgtaccg ttaaatattg aattgacaca tatcgctgcg acacatactg
     5761 ccggaatatt attgtgtacg ttatgtttac tgcaatttat tgtcagcctg atgattgaga
     5821 atcgctatga gcataatctg acttcatcgc ttttctggat tatttggttc ccggttattt
     5881 tctggatgct gagcctggca acgacattgg tatcatttac acgagtcatg ttgatgccta
     5941 aaaagcaacg cgcccgttgg gtaagtcccg atcgcgggat tctgagaggt taatatgaac
     6001 aatttaatta ttacgacccg acaatcgcca gtacgtttac tggttgatta tgttgccaca
     6061 accatcttgt ggacattatt tgctttgttc atattcttat tcgccatgga tctgctgacg
     6121 ggttattact ggcaaagcga ggccagaagc cgacttcagt tctatttttt gctggcagtg
     6181 gcgaatgccg tcgtgttaat tgtctgggcg ctgtacaata agctgcgttt tcaaaaacag
     6241 cagcatcatg cagcctacca atatacgccg caagaatatg cagagagctt agcaatacct
     6301 gatgagctct atcagcaact acaaaaaagc cacaggatga gcgtacactt caccagccag
     6361 gggcaaataa aaatggtggt ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca
     6421 aagcccggtt cgcccgggct ctgcaccgat aatacactta actgtatgca tgcagcgtac
     6481 gttggcaaag tgccgaacgt acgcaatcct ctttaccgaa ccggacgatc ccgaccattt
     6541 catcttcttc gaaacgttcc agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat
     6601 cgcattgcgt gatatcaccg ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa
     6661 acattttcat ttgcgcggca gtcacattct gcgcctcgtc aagaatgacg actgcatttt
     6721 caaaggtacg tccacgcata taggcgaacg gcgcaatttc caccttccct atttccggtc
     6781 gcaggcagta ctgcataaag gaagccccta agcgccggac cagcacgtcg tagaccgggc
     6841 gaaaataggg agcaaacttt tctgcgatat ctccaggtaa gaagccaaga tcttcatcgg
     6901 cttgcagaac tggacgggtg acgataatcc tgtcgacatc cttatgtatc agggcctctg
     6961 ccgcttttgc tgcgctgatc caggtttttc cgcacccggc ttcgcccgtg gcgaatatca
     7021 gctgcttact ctcaatagcc ttcagatagt gcaattgcgc ttcatttcgc gcgaggatgg
     7081 gcgaggtatc gcgactgtcg cgggccatac caatggcttc tacgccgctc atctgcacaa
     7141 gcgaggtgac cgattcttct tcacgctgct tatggctgcg cgaatcccgt ctcagcacac
     7201 gttttgcctc gcgacgagct ttgatcactg ctttttgtct tcccatggag agcaccttga
     7261 gttgtttgta ttcatcacac gcgccgttgg cagcgcgatt atgcgcacga acatcagagg
     7321 gttggcttcc ttgtaagcca tagtttgctt ttggataaaa tgccgaaaaa cggctacgcg
     7381 caccgtttac ggcgtcggta acacatgaaa agaaaggatg aggttgaaaa tgcaaagtga
     7441 cgagatgact accggaggag aaaactccgc gagtggtggc gcgttgatta tctaaaacat
     7501 gtccagtaca ggacgttacc atccgcgatc tccatagtga ctgactatca ctgccgggaa
     7561 cttccgctgc tacttaataa gtacaacaga tctcgcattc attgcaacaa tatattcact
     7621 tatatttaac tataaaacac cattttagtg acattagttt ctactggaaa gatgacagag
     7681 tgatgacaga gtgatgacag agatgaaaaa agctgtgtgc tttcagcagg attcacatca
     7741 ggcatcagtg cacgaacgtc ggatgcggcg tgaacgcctt atccagccta ccaaacccgc
     7801 accgattgtg ggcaacataa gcatcgcaag cgccgcatca ggcatctgcg cacaaatgcc
     7861 ttatccgcct aaaaacatta aacccgcaat aatgcgcttc cgagataatc gttcgcgtcc
     7921 ttcactcccg gcagcgcaaa aaaataaccg ccgccgatag gtttaacgta ttcctccagc
     7981 gcttcgccat tgagcctttt ttgtactgtc aggaagcctt ttaccagatc gtgctggtag
     8041 cagacaaaca gcaaccccat atccagttgt ccggagttgg tgacgcccag tgaataactg
     8101 tagccacgac gcagcatcag gctggactca ctctccgccg tgcggggatt cgccagccgg
     8161 atatggctgt ccagcgcgat caccttccct tccgggtcgc tggcgtaatc aggcacatca
     8221 tgctcatgct gcattcccag cggcgcaccg gtttgcttat cacggccaaa aatcgtctgc
     8281 tgttctttca gcggcgttct gtcccaaaat tccactcgaa actgaatcaa gcgtactgcc
     8341 tgatagctgc caccgattgt ccacgcaggc tcctgctgat ctgccgttac ccacaccact
     8401 ttttgcatca acttatcatt ctggctatcg ggattggcag tgccgtcttt gaaacccagc
     8461 aaattaatcg gcgtctcttt gcctttacta cgcgccgcgt gatcggaaat aaacccttcc
     8521 cgcttccagc gcacactgag caaatccggc gtgtgtttga tgatatcgcg cagcgcatgg
     8581 atcaccgtgt cctgggtgtt ggcgcaaatt tgtagcaaca catcaccatg gcataacgcc
     8641 gcatccagcg agtcgttggg gaaacgcgtc atcttctgca actttttcgg catctgtggc
     8701 gcaaggccga agcgctcatc aaacaatgag tgacccaccg ataacgtgat ggtgagatta
     8761 tcgggcgcaa tgtagccgcc aagaatgccg gaatcgagtg gtggcaggcg cggatttggc
     8821 gtttctggtg ctgctccgcc ctgagtcaga aaagcaaaac gctgagtcaa caagcgaaac
     8881 aaccgctcaa gatcggcttt atcgctggca agcacatcaa acgccaccag catcattgcg
     8941 gcctgttgtg gcgtcaggat ccctgcctga tgctcaccat aaaacggctg tttctcattg
     9001 cgtgcatccg gtgaaagcgt gcccggtgca ctttgcgttt tttgtgcatg agcgaccgga
     9061 caacttcccg ccagcgccag cgcaccaatt cctttcagta aacgtcggcg tgacagttca
     9121 ttcacgccgt tttcatcttc atactgcata acgcttaatc cagtcccagc acaccacgaa
     9181 gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcgttc cggtcggcct
     9241 cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc agaatggtat
     9301 cgaccttttt aaagttggca tcgacttttg ccagcagttc cgggttggct ttttgcagtt
     9361 gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg aaatcccaca
     9421 gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggccgcc acttcctcaa
     9481 tcagtccggc tgcaccgcca accacttttg aaggtgggaa agccagttca ctgatgcgtt
     9541 tttgtaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatt cctttggtgg
     9601 tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc ggtgaatttt ggatcagcgg
     9661 ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg gagaacagtt
     9721 cagcaatcgg ctcaatgcgc tcatagtgct ggcgcgtcgg cgcatacagt gctttcgctt
     9781 tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc accagctgtg
     9841 tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga cttaacagcg
     9901 catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc cctttcgggt
     9961 tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc gtcattttct
    10021 ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct ttgaggatct
    10081 cccactccag cgccttctgg ctgtggttct gaataatgaa ctgtgttttc ccggcgttaa
    10141 ccgtaatggt catcggttcg cactgtttat ccgtcacggt cactttgact tgcggaatat
    10201 cagcggcgtt agccataaaa gcagaagaga acagcgcagc cacgctcaac tgcaatgcgt
    10261 tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg taactaagca
    10321 agagtttgcg tcgtatttgc tacgcggagc gagacgctgt cgcccctgcg cgtggtggca
    10381 gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact tcgctgacgc
    10441 tcggcgcttc ctgataacca aaaatccctt ccatcaacgt gccaaacagc gagtgagttg
    10501 agagcaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat ccggcttcat
    10561 gaaatgcgcg aatggcacct gcagccagcc ctgcggcgac gaagagaata aacaggctgg
    10621 tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc aggaagccga
    10681 gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc ccgacatctt
    10741 gttgaaatgc cgccagcagg aaaaagaccg actccaggcc ttcccttgca acggcaaaaa
    10801 agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg ctatcgactg
    10861 cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa accatccagg
    10921 taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc tctttttgcg
    10981 gaaattcgcc ggtggtttcg ttaatgaaga tacccaagcc caggcacaac gcagcggcaa
    11041 gcaacacgcc aatccacatc acaccaatcc atcggcctcg ctgggtacgc ttaagatagc
    11101 tggcaatcaa actgacaatc agcgcggctt caagcccttc gcgcaacata atgagaaatg
    11161 gaacaaacat gccacccacc cttaaacgtt tttctgagtc aatctatgca aagaaaagta
    11221 aatcgcagtg atagcgatta tcattacgct aaagaaaaat acaagcggaa tgtttataaa
    11281 atgatgttgg aatgtaaaaa tgagtagatc aaatccggag aaaaaagcga ttattttaag
    11341 gtatttagct gacgtttcgc ccggcagatg aagcttgtac gaatgttggt ggcgacaaac
    11401 tcgctgcagc gtgccccgaa gggttgaatt ttcagttagt cgtatcacaa aagaacgttg
    11461 ttttttgctt attaatagat agtgataggg ttgtgaatga aattatacct cgtctcctca
    11521 tcgaattttt cgcccacaac ctaaacagga gaaaacgtgt atttaa
//
LOCUS       DAZKFU020000144        11455 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZKFU020000144 DAZKFU020000000
VERSION     DAZKFU020000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.145"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(58..1422)
                     /gene="rhlE"
                     /locus_tag="ACHOQQ_RS15815"
                     /old_locus_tag="pgaptmp_003163"
     CDS             complement(58..1422)
                     /gene="rhlE"
                     /locus_tag="ACHOQQ_RS15815"
                     /old_locus_tag="pgaptmp_003163"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGU4471376.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQLRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            1651..2322
                     /gene="cecR"
                     /locus_tag="ACHOQQ_RS15820"
                     /old_locus_tag="pgaptmp_003164"
     CDS             1651..2322
                     /gene="cecR"
                     /locus_tag="ACHOQQ_RS15820"
                     /old_locus_tag="pgaptmp_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGU4471377.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2325..3320
                     /gene="hlyD"
                     /locus_tag="ACHOQQ_RS15825"
                     /old_locus_tag="pgaptmp_003165"
     CDS             2325..3320
                     /gene="hlyD"
                     /locus_tag="ACHOQQ_RS15825"
                     /old_locus_tag="pgaptmp_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGU4471378.1"
                     /translation="MKKPVVIGLAVVALAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3313..5049
                     /gene="ybhF"
                     /locus_tag="ACHOQQ_RS15830"
                     /old_locus_tag="pgaptmp_003166"
     CDS             3313..5049
                     /gene="ybhF"
                     /locus_tag="ACHOQQ_RS15830"
                     /old_locus_tag="pgaptmp_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4471379.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5042..6175
                     /gene="ybhS"
                     /locus_tag="ACHOQQ_RS15835"
                     /old_locus_tag="pgaptmp_003167"
     CDS             5042..6175
                     /gene="ybhS"
                     /locus_tag="ACHOQQ_RS15835"
                     /old_locus_tag="pgaptmp_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4471380.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6186..7292
                     /gene="ybhR"
                     /locus_tag="ACHOQQ_RS15840"
                     /old_locus_tag="pgaptmp_003168"
     CDS             6186..7292
                     /gene="ybhR"
                     /locus_tag="ACHOQQ_RS15840"
                     /old_locus_tag="pgaptmp_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4471381.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTYQTVPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7254..7664)
                     /gene="ybhQ"
                     /locus_tag="ACHOQQ_RS15845"
                     /old_locus_tag="pgaptmp_003169"
     CDS             complement(7254..7664)
                     /gene="ybhQ"
                     /locus_tag="ACHOQQ_RS15845"
                     /old_locus_tag="pgaptmp_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGU4471382.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7797..8558
                     /gene="ybhP"
                     /locus_tag="ACHOQQ_RS15850"
                     /old_locus_tag="pgaptmp_003170"
     CDS             7797..8558
                     /gene="ybhP"
                     /locus_tag="ACHOQQ_RS15850"
                     /old_locus_tag="pgaptmp_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4471383.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8555..9796
                     /gene="clsB"
                     /locus_tag="ACHOQQ_RS15855"
                     /old_locus_tag="pgaptmp_003171"
     CDS             8555..9796
                     /gene="clsB"
                     /locus_tag="ACHOQQ_RS15855"
                     /old_locus_tag="pgaptmp_003171"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGU4471384.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9796..10752
                     /gene="ybhN"
                     /locus_tag="ACHOQQ_RS15860"
                     /old_locus_tag="pgaptmp_003172"
     CDS             9796..10752
                     /gene="ybhN"
                     /locus_tag="ACHOQQ_RS15860"
                     /old_locus_tag="pgaptmp_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGU4471385.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGENTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10788..11447)
                     /gene="ybhM"
                     /locus_tag="ACHOQQ_RS15865"
                     /old_locus_tag="pgaptmp_003173"
     CDS             complement(10788..11447)
                     /gene="ybhM"
                     /locus_tag="ACHOQQ_RS15865"
                     /old_locus_tag="pgaptmp_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4471386.1"
                     /translation="MLNGIWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGL
                     LLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLIS
                     MLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWSGIISH
                     GRNKLLELAGKCHSEELWSPVRCAFIGALTLYYYFIGFFGILAAIAITLVWQRHTRFF
                     H"
BASE COUNT         2643 a         3056 c         3106 g         2650 t
ORIGIN      
        1 ccgtgttacc tgaataaagg gcaaaagcct gtgatactct tttgccctta catggtatta
       61 ctgcgcagcg gcaggtttac gcggacggcg gcgacgttgt tgttcgcctg ccggtttggc
      121 atcgccgagg cggcgagacg gtttttctgc aggcttcgcg cttgcagatt ttgcaccacc
      181 ttctccacgg cgtagttgct gttgaccgcg accaccgccc tgcccacgac caccgccgcc
      241 acgttgctgg cgaccgttct ggatcggttc ggctttgatt gacgggtccg gctcatagcc
      301 cggaatcgca atgcgcggga tctctttttt cagcagtttt tcgatatcac gcagcagttt
      361 gtgttcatca acacacacca acgacaacgc ttctccggtg gctgcggcac gaccggtacg
      421 cccgatacgg tggacataat cttccggtac gtttggcagt tcatagttga cgacgtgcgg
      481 caactcttcg atatccagac cgcgcgcagc gatgtcagtt gccaccagta cacgaatatc
      541 gcccgattta aaatcagcca gcgcacgggt acgcgcacct tgcgatttat taccgtggat
      601 cgccgcacta cggatgccat ctttattaag ctgttcagcg agatggttag cgccatgttt
      661 ggtacgggta aacaccagca cctgctgcca gttccctttc ccaatcatgt gcgacagcaa
      721 ttcgcgtttg cgcttcttat caacaaagtg aacatgctgg gtcacctgat cagacgcggt
      781 attgcggcgt gccacttcga tttccagcgg gttgtgcaac agtttttccg ccagggcttt
      841 aatatcgtca gagaaggtcg cggagaacaa caggttctgg cgcttcgcag gtagttttgt
      901 taacacgcga cggatatcgt ggataaagcc catgtcgagc atgcggtcag cttcatcgag
      961 gacgaggatt tcaacctgat ccagcttcac tgcattctga tgttccaggt ccagcaaacg
     1021 tcccggcgtt gccaccagca catcaacgcc gccgcgcaac ttcatcattt gtgggttaat
     1081 gctgacaccg ccaaacacca ccagcgaacg aatgttcaga tatttgctgt aatcacggac
     1141 gttttcgcca atctgcgcag ccagttcacg ggtcggggta agaatgagag cacgtaccgg
     1201 acgacgccct ttggcgtgcg gctggcgagt gatcaggtgt tgcaacagcg gcagcgtaaa
     1261 gcccgctgtt ttgccggtgc cggtctgggc gctagccatc aggtcgcggc cttccagcac
     1321 cgcagggatc gcctgctgct gaataggggt gggttcacgg taaccctgct cggcaacggc
     1381 gcgcaggata tcagggctta aacccaaaga atcgaaagac ataactactc cgaaccgccc
     1441 cgaccgttac cggtgtagtt ttcagggaga tactgaaaag gaaaatgaca aaaaccacaa
     1501 tgtcatgagg gagcggagtg tagcagtttt tgtgacgcag cgcataaatt atccctgtga
     1561 ctggcgtagc taaaattctt agccatactg gacaactccc ctatggagtc ataatcttaa
     1621 tcaatcattt gattaagaat ggactgtgcg atgaataatc ctgccatgac aatcaagggt
     1681 gaacaggcga aaaaacagct gattgctgcc gcactggcgc agtttggtga atatggaatg
     1741 aacgccacca cccgcgagat agccgcccag gccgggcaga atatcgctgc catcacctac
     1801 tacttcggtt cgaaagaaga tttgtacctc gcctgcgccc agtggattgc cgattttatt
     1861 ggcgagcagt tccgtccgca tgccgaggaa gccgaacgct tgttcgccca accacagcct
     1921 gatcgggctg ccatccgtga actgatcctt cgcgcctgca ggaacatgat taagctgctc
     1981 acccaggatg acaccgtcaa cctcagcaag tttatctccc gtgagcagct ctctcccacg
     2041 gcagcctacc acctggtgca cgaacaggtg attagtccgc tacacagcca cctgacacgc
     2101 ctgattgccg cctggaccgg ctgcgacgcc aatgacaccc gcatgatcct tcatacccat
     2161 gcgttgattg gtgagattct ggcgttccgt cttggcaaag aaacaattct gttacgtacc
     2221 ggctggaccg cgttcgatga agaaaagacc gaactgatca accagacggt gacctgtcat
     2281 atcgacctga ttttgcaagg attatcgcaa aggagtttgt agtgatgaaa aaacctgtcg
     2341 tgatcggact ggcggtagtg gcacttgccg ccgtggttgc cggaggctac tggtggtatc
     2401 aaagccgcca ggataacggc ctgacgctgt atggcaacgt ggatattcgt acggtgaatc
     2461 ttagtttccg tgttgggggg cgcgttgaat cgctggcggt ggacgaaggt gatgcgatca
     2521 aagcgggcca ggtgctgggc gaactggatc acaagccgta tgagattgcc ctgatgcagg
     2581 cgaaagcggg cgtctcggtg gcacaggcgc agtatgacct gatgcttgcc gggtatcgcg
     2641 atgaagaaat cgctcaggcc gccgctgcgg tgaaacaggc gcaagccgcc tatgactatg
     2701 cgcagaactt ctataaccgc cagcaaggac tgtggaaaag ccgcaccatt tcggcaaatg
     2761 acctggaaaa tgcccgctcc tcgcgcgacc aggcacaggc aacgctgaaa tcagcacagg
     2821 ataaattgcg tcagtaccgt tccggtaacc gtgaacagga catcgcccag gcgaaagcca
     2881 gcctcgaaca ggcgcaggcg caactggcgc aggcggagct gaatttacag gactcaacgt
     2941 tgatagcacc gtctgatggc acgctgttaa cgcgcgcggt ggagccaggc acggttctca
     3001 atgaaggtgg cacggtgttt accgtttcac taacgcgtcc ggtgtgggtg cgcgcttatg
     3061 ttgatgaacg gaatcttgac caggcccagc cggggcgcaa agtgctgctt tataccgatg
     3121 gtcgcccgga caagccctat cacgggcaga ttggtttcgt ttcgccgact gctgaattta
     3181 ccccgaaaac cgtcgaaaca ccggatctgc gtaccgacct cgtctatcgc ctgcgtattg
     3241 tggtgaccga cgccgatgat gcgttacgcc agggaatgcc agtgacggta caattcggtg
     3301 acgaggcagg acatgaatga tgccgttatc acgctgaacg gcctggaaaa acgctttccg
     3361 ggcatggaca agcccgccgt cgcgccgctc gattgcacca ttcatgccgg ttatgtgacg
     3421 gggctggtag ggccagacgg tgcgggtaaa accacgctga tgcggatgct ggcgggatta
     3481 ctgaaacctg acagcggcag tgccacggtg attggctttg atccgatcaa aaatgacggc
     3541 gcgctgcacg ccgtgctcgg ctatatgccg cagaaatttg gtctgtatga agatctgacg
     3601 gtgatggaga acctcaatct gtacgcggat ttgcgcagcg tcaccggcga ggcacgtaag
     3661 caaacttttg ctcgcctgct ggagtttacg tctcttggcc cgtttaccgg acgcctggcg
     3721 ggcaagctct ccggtgggat gaaacaaaaa ctcggcctgg cctgtaccct ggtgggcgaa
     3781 ccgaaagtgt tgctgctcga tgaacccggc gtcggcgttg accctatctc acggcgcgaa
     3841 ctgtggcaga tggtgcatga gctggcgggc gaagggatgt taatcctctg gagtacctcg
     3901 tatctcgacg aagccgagca gtgccgtgac gtgttactga tgaacgaagg cgagttgctg
     3961 tatcagggag aaccaaaagc cctgacacaa accatggccg gacgcagctt tctgatgacc
     4021 agtccacacg agggcaaccg caaactgttg caacgcgcct tgaaactgcc gcaggtcagc
     4081 gacggcatga ttcaggggaa atcggtacgt ctgatcctca aaaaagaggc cacaccagac
     4141 gatattcgcc atgccgacgg gatgccggaa atcaacatca acgaaactac gccgcgtttt
     4201 gaagatgcgt ttattgattt gctgggcggt gccggaacct cagaatcgcc gctgggtgca
     4261 atattgcata cggtagaagg cacacccggc gagacggtga tcgaagcgaa agaactgacc
     4321 aagaaatttg gtgattttgc cgccaccgat cacgtcaact ttgccgttaa acgcggggag
     4381 atttttggtt tgctggggcc aaacggcgcg ggcaaatcga ccacctttaa gatgatgtgc
     4441 ggtttgctgg tgccgacttc cggccaggcg ctggtgctgg ggatggatct gaaagagagt
     4501 tccggtaaag cgcgccagca tctcggctat atggcgcaaa aattttcgct ctacggcaac
     4561 ctgacggtcg aacagaattt acgctttttc tctggtgtgt atggcttacg cggtcgggcg
     4621 cagaacgaaa aaatctcccg tatgagcgag gcatttggcc tgaaaagtat tgcctcacac
     4681 gccaccgatg aactgccatt aggttttaaa cagcggctgg cgctggcctg ttcgctgatg
     4741 catgaaccgg atattctgtt tctcgacgaa ccgacttccg gcgttgaccc cctcacccgc
     4801 cgtgaattct ggctgcacat caacagcatg gtagagaaag gcgtcacggt gatggtcacc
     4861 acccacttta tggatgaagc ggaatattgc gaccgcatcg gcctggtgta ccgcgggaaa
     4921 ttaatcgcca gcggcacgcc ggacgatttg aaagcacagt cggctaacga tgagcaaccc
     4981 gatcccacca tggagcaagc ctttattcag ttgatccacg actgggataa ggagcatagc
     5041 aatgagtaac ccgatccttt cctggcgtcg cgtgcgggcg ctgtgcgtca aagagacgcg
     5101 gcagatcgtt cgcgatccga gtagctggct gattgcggta gtgatcccgc tgttgctgct
     5161 gtttattttt ggttacggca ttaacctcga ctccagcaag ctgcgggtcg ggattttact
     5221 ggaacagcgt agcgaagcgg cgctggattt cacccacact atgaccggtt cgccctacat
     5281 cgacgccacc atcagcgata accgtcagga actgatcgcc aaaatgcagg cggggaaaat
     5341 tcgcggtctg gtggttattc cggtggattt tgcggaacag atggagcgcg ccaacgccac
     5401 cgcaccgatt caggtgatca ccgacggcag tgagccgaat accgctaact ttgtacaggg
     5461 gtatgtcgaa gggatctggc agatctggca aatgcagcga gcggaggaca acgggcagac
     5521 ttttgaaccg cttattgatg tacaaacccg ctactggttt aacccggcgg cgattagcca
     5581 gcacttcatt atccccggtg cggtgaccat catcatgacg gtcatcggcg cgattctcac
     5641 ctcgctggtg gtggcacgag aatgggaacg cggcaccatg gaggctctgc tctctacgga
     5701 gattacccgc acggaactgc tgctgtgtaa gctgatccct tattactttc tcgggatgct
     5761 ggcgatgttg ctgtgtatgc tggtgtcagt gtttattctc ggcgtgccgt atcgcggttc
     5821 gctgctgatt ctgtttttta tctccagcct gtttttactc agtaccctgg ggatggggct
     5881 gctgatttcc acgattaccc gcaaccagtt caatgccgct caggtcgccc tgaacgccgc
     5941 gttcctgccg tcgattatgc tttccggctt tatttttcag atcgacagta tgcccgcggt
     6001 gatccgcgcg gtgacgtaca ttattcccgc tcgttatttc gtcagcaccc tgcaaagcct
     6061 gttcctcgcc gggaatattc cagtggtgct ggtggtaaac gtgctgtttt tgatcgcttc
     6121 ggcggtgatg tttatcggcc tgacgtggct gaaaaccaaa cgtcggctgg attagggaga
     6181 agagcatgtt tcatcgctta tggacgttaa tccgcaaaga gttgcagtcg ttgctgcgcg
     6241 aaccacaaac ccgcgcgatt ctgattttac ccgtgctaat tcaggtgatc ctgttcccgt
     6301 tcgccgccac gctggaagtg actaacgcca ccatcgccat ctacgatgaa gataacggcg
     6361 agcattcggt ggaacttact caacgttttg cccgcgccag cgcctttaca catgtgctgc
     6421 tgctgaaaag cccacaggag atccgcccca ccatcgacac acaaaaggcg ttactactgg
     6481 tgcgtttccc ggctgacttc tcgcgcaaac tggataccta ccagaccgtg ccgttgcagt
     6541 tgatcctcga cgggcgtaac tccaacagtg cgcaaattgc cgccaactac ctgcaacaga
     6601 tcgtcaaaaa ttatcagcag gaactgctgg aaggaaaacc gaaacccaac aacagcgagc
     6661 tggtggtacg caactggtat aacccgaatc tcgactacaa atggtttgtg gtgccgtcgc
     6721 tgatcgccat gatcaccact atcggcgtaa tgatcgtcac ttcactttcc gtcgcccgcg
     6781 aacgtgaaca aggtacgctc gatcagctac tggtttcgcc gctcaccacc tggcagatct
     6841 tcatcggcaa agccgtaccg gcgttaattg tcgccacctt ccaggccacc attgtgctgg
     6901 cgattggtat ctgggcgtat caaatcccct tcgccggatc gctggcgctg ttctacttta
     6961 cgatggtgat ttatggttta tcgctggtgg gattcggtct gttgatttca tcactctgct
     7021 cgacacaaca gcaggcgttt atcggcgtgt ttgtttttat gatgcccgcc attctccttt
     7081 ccggttacgt ttctccggtg gaaaacatgc cagtatggct gcaaaacctg acgtggatta
     7141 accctattcg ccactttacg gacattacca agcagattta tttgaaggat gcgagtctgg
     7201 atattgtgtg gaatagtttg tggccgctac tggtgataac ggccacgaca gggtcagcgg
     7261 cgtacgcgat gtttagacgt aaggtgatgt aacttcttat ctttcgccag caaagacact
     7321 accgccgggc cggcaaggat tgccagccct gcaattgcca gcaaaaagtt gttttccaga
     7381 acgcgggaca acagccacag cacaatcagc gctgcgccaa aataccaggt cgtggtcagc
     7441 tcttccagca cgtctgccac ctcacgccag cgttgctcgg ggtggcgctg aaacaccagc
     7501 atcatcacta ccgtgacaca atacagtgcg cataatccca cgccgacgtg aaccagagtc
     7561 ttactcatcc agcccaccac cagcagcgcc actaccagta aatgcaacat aatctgccag
     7621 caacttagac ccgttgcgac acgaacacgt tgttgccact tcatggcttc tctcctgaag
     7681 aatttatctc tacttactca gagtaccgaa ctttacggaa aattccgtaa gaccgcacct
     7741 ttttgtgacg ccgactacac tatttcaatg agataagagg caaagacagg aaaattatgc
     7801 ccgatcaaac acaacaattt tcgttcaagg tgctcaccat caatattcac aaaggcttta
     7861 ccgcgtttaa ccgacgcttc attttgccgg aacttcgcga tgccgtgcgt accgtcagcg
     7921 ccgatattgt ttgcctgcag gaagtgatgg gcgcgcacga agttcatccg ctgcatgtgg
     7981 aaaactggcc cgatacctcg cactacgagt ttctcgccga cactatgtgg agcgattttg
     8041 cctacggtcg caatgccgta tacccggaag ggcatcacgg caacgccgta ctgtcgcgtt
     8101 atcccattga acattatgag aatcgcgatg tttcggtcga tggtgcggaa aagcgcggcg
     8161 tgctctactg ccgcattgtg ccgccgatga ccggaaaagc gattcatgtg atgtgcgtac
     8221 atctgggcct gcgtgaggcg caccgtcagg cgcagcttgc gatgctcgcc gaatgggtga
     8281 atgagctacc ggacggcgaa ccggtattgg tggcgggtga tttcaatgac tggcggcaaa
     8341 aagctaatca tccgttaaaa gtgcaggccg gactggatga gatttttacc cgcgcccacg
     8401 gacgcccggc gcgcacgttt ccggtgcaat ttcctctact acgactggac aggatctacg
     8461 tcaaaaatgc cagcgccagc gcgccaaccg cgttgccgct gcggacatgg cgacaccttt
     8521 ctgatcatgc ccctttaagt gcggagattc atttatgaaa tgtagctggc gcgaaggcaa
     8581 taagatccag ttgctggaaa acggcgagca atattatccc gcggtgttta aggcgattgg
     8641 cgaggcacaa gaacgcatca ttcttgaaac gtttatctgg tttgaggatg acgtcggcaa
     8701 acaactgcat gcggcactac tggcagcagc gcaacgcggg gttaaagcgg aagtcttgct
     8761 ggatggctac ggttcgccgg atctcagcga tgagtttgtc aatgaactga cggcagctgg
     8821 cgtggtgttc cgctactacg atccccgccc tcgccttttt ggtatgcgca ccaatgtgtt
     8881 tcgccggatg catcgcaaaa ttgtggtgat cgatgcgcgt atcgccttta ttggcgggct
     8941 gaattattcc gccgagcata tgtccagcta cggtccagag gccaaacagg attacgcggt
     9001 gcgccttgaa gggccgattg tcgaagatat tctccagttt gagctggaaa acctgcctgg
     9061 acagagcgcg gcacgacgct ggtggcgacg tcatcacaaa gcggaagaga accgccagcc
     9121 gggagaagcg caggtattgc tggtctggcg cgataacgaa gaacatcgcg atgatattga
     9181 acgtcactat ctgaaaatgc tcactcaggc gcgtcgggaa gtgattattg ccaacgccta
     9241 cttcttcccc ggctatcgat ttttacacgc cttgcgtaaa gcggcacggc gcggggtgcg
     9301 gatcaaactg atcattcagg gcgaaccgga tatgccgatt gtcagagtcg gtgcgcgctt
     9361 gctgtataac tatctggtta aaggcggcgt tcaggtgttt gagtaccgcc gccgcccgct
     9421 ccacggcaaa gtggcattga tggacgatca ctgggcgaca gtagggtcca gtaatctcga
     9481 tccgctcagt ttgtcactga atctcgaagc aaatgtcatc atccacgatc gtcattttaa
     9541 ccagacgctg cgcgataatc tgaacggcat tattgccgca gattgtcagc aggtcgatga
     9601 gaccatgctg ccgaaacgca cctggtggaa cctaaccaaa agcgtgctgg cgttccactt
     9661 tttacgccac ttcccggcgc tggttggctg gcttccggca cacacgccac gtctggcgca
     9721 ggttgatccc cccgcacaac cgacaatgga aacgcaggat cgggtagaaa ctgaaaacac
     9781 gggggtaaaa ccctgatgag taaatcacac ccgcgctggc gcttagcaaa gaagatcctc
     9841 acctggctgt tttttatcgc ggtgattgtg ttgctggtgg tctacgccaa aaaagtggac
     9901 tgggaagagg tctggaaggt catccgcgac tacaatcgcg ttgcgctgct tagtgcggtc
     9961 gggctggtgg tcgtcagcta tctgatttac ggctgctatg acctgctcgc ccgcttttac
    10021 tgcggtcaca aactggcgaa gcgccaggtg atgctggtgt cgtttatctg ctacgccttc
    10081 aacctgacgc tcagtacctg ggtcggcggc attggtatgc gctatcgttt gtactctcgt
    10141 ctggggttgc ccggtagcac tattacgcgg attttctcgc tcagtattac caccaactgg
    10201 ctgggctata ttttgctggc ggggattatc tttaccgcag gcgtggtgga gctaccggat
    10261 cactggtatg tcgatcaaac cacactgcgc attctcggca ttggcttact gatgattatc
    10321 gcggtttatt tgtggttttg cgctttcgcg aagcaccgcc atatgaccat caaagggcaa
    10381 aagctggtgc tgccctcatg gaaattcgcc ctcgcccaga tgttgatttc cagtgttaac
    10441 tggatggtca tgggagcgat tatctggctg ttacttggtc aaagcgtgaa ctatttcttt
    10501 gtactgggcg tgttgctggt tagtagtatt gcaggcgtca tcgtgcatat tccggcgggg
    10561 attggtgtgc tggaagcggt atttatcgca ttactggcag gggagaatac atccaaaggc
    10621 acaattatcg ccgccctgct cgcttatcgc gtcctgtatt actttatccc gctgctgctg
    10681 gcgctgatct gttacctgct actggaaagc caggcgaaga agctacgggc gaaaaatgaa
    10741 gcggcgatgt gaagttcagt taagttcttt agtatgtgca tttacgacta atgaaaaaaa
    10801 cgcgtatgcc tttgccagac aagcgttata gctatcgcgg caagtatccc aaagaagccg
    10861 ataaaatagt aatagagtgt taatgcacct ataaaagcgc aacgaaccgg actccacaac
    10921 tcttcactat ggcatttccc cgccagttca aggagcttat ttcgtccatg cgagataatg
    10981 cctgaccata acacaatcat taagcaactt attacccaaa tgggccgttc actcattaat
    11041 gccgcattca cgatgattac cagggccaac cctgtcaacg tcatcataat gataaaacgg
    11101 tgagaaccag gatcaacatt aaataataaa ccggccagca tactgattaa aaacatcgcg
    11161 cccgcagtgc tgaacacgat gataatggca tatacaggaa ctaaacttgc aacgacaatc
    11221 gccaccaaag agagtaaaaa cagatatatt acgcctgcga tggcggcatt tatcttgttg
    11281 atatccttac ttaagccata catacaaagt aataataacc ctatggcgac atacattggt
    11341 ggtgaatcat atttggttgc cgtaatttcg agatacttag caccccaggc cagccctgcg
    11401 gttgccacca aacctaaagc ggtaatgagc catataccgt ttaatatcct ggtct
//
LOCUS       DAZKFU020000145        11293 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZKFU020000145 DAZKFU020000000
VERSION     DAZKFU020000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.146"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            229..810
                     /gene="mdaB"
                     /locus_tag="ACHOQQ_RS15870"
                     /old_locus_tag="pgaptmp_003174"
     CDS             229..810
                     /gene="mdaB"
                     /locus_tag="ACHOQQ_RS15870"
                     /old_locus_tag="pgaptmp_003174"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGU4471387.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            841..1155
                     /gene="ygiN"
                     /locus_tag="ACHOQQ_RS15875"
                     /old_locus_tag="pgaptmp_003175"
     CDS             841..1155
                     /gene="ygiN"
                     /locus_tag="ACHOQQ_RS15875"
                     /old_locus_tag="pgaptmp_003175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGU4471388.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1203..3095)
                     /gene="parE"
                     /locus_tag="ACHOQQ_RS15880"
                     /old_locus_tag="pgaptmp_003176"
     CDS             complement(1203..3095)
                     /gene="parE"
                     /locus_tag="ACHOQQ_RS15880"
                     /old_locus_tag="pgaptmp_003176"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGU4471389.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3124..3705)
                     /gene="yqiA"
                     /locus_tag="ACHOQQ_RS15885"
                     /old_locus_tag="pgaptmp_003177"
     CDS             complement(3124..3705)
                     /gene="yqiA"
                     /locus_tag="ACHOQQ_RS15885"
                     /old_locus_tag="pgaptmp_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGU4471390.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3705..4532)
                     /gene="cpdA"
                     /locus_tag="ACHOQQ_RS15890"
                     /old_locus_tag="pgaptmp_003178"
     CDS             complement(3705..4532)
                     /gene="cpdA"
                     /locus_tag="ACHOQQ_RS15890"
                     /old_locus_tag="pgaptmp_003178"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGU4471391.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4557..4979)
                     /gene="yqiB"
                     /locus_tag="ACHOQQ_RS15895"
                     /old_locus_tag="pgaptmp_003179"
     CDS             complement(4557..4979)
                     /gene="yqiB"
                     /locus_tag="ACHOQQ_RS15895"
                     /old_locus_tag="pgaptmp_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGU4471392.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(4980..5609)
                     /gene="nudF"
                     /locus_tag="ACHOQQ_RS15900"
                     /old_locus_tag="pgaptmp_003180"
     CDS             complement(4980..5609)
                     /gene="nudF"
                     /locus_tag="ACHOQQ_RS15900"
                     /old_locus_tag="pgaptmp_003180"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGU4471393.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWV"
     gene            5814..7295
                     /gene="tolC"
                     /locus_tag="ACHOQQ_RS15905"
                     /old_locus_tag="pgaptmp_003181"
     CDS             5814..7295
                     /gene="tolC"
                     /locus_tag="ACHOQQ_RS15905"
                     /old_locus_tag="pgaptmp_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGU4471394.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7443..8114
                     /gene="ygiB"
                     /locus_tag="ACHOQQ_RS15910"
                     /old_locus_tag="pgaptmp_003182"
     CDS             7443..8114
                     /gene="ygiB"
                     /locus_tag="ACHOQQ_RS15910"
                     /old_locus_tag="pgaptmp_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGU4471395.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8120..9280
                     /gene="ygiC"
                     /locus_tag="ACHOQQ_RS15915"
                     /old_locus_tag="pgaptmp_003183"
     CDS             8120..9280
                     /gene="ygiC"
                     /locus_tag="ACHOQQ_RS15915"
                     /old_locus_tag="pgaptmp_003183"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4471396.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9318..10106)
                     /gene="ygiD"
                     /locus_tag="ACHOQQ_RS15920"
                     /old_locus_tag="pgaptmp_003184"
     CDS             complement(9318..10106)
                     /gene="ygiD"
                     /locus_tag="ACHOQQ_RS15920"
                     /old_locus_tag="pgaptmp_003184"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HGU4471397.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10249..11022
                     /gene="zupT"
                     /locus_tag="ACHOQQ_RS15925"
                     /old_locus_tag="pgaptmp_003185"
     CDS             10249..11022
                     /gene="zupT"
                     /locus_tag="ACHOQQ_RS15925"
                     /old_locus_tag="pgaptmp_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGU4471398.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11080..11250)
                     /gene="yqiD"
                     /locus_tag="ACHOQQ_RS15930"
                     /old_locus_tag="pgaptmp_003186"
     CDS             complement(11080..11250)
                     /gene="yqiD"
                     /locus_tag="ACHOQQ_RS15930"
                     /old_locus_tag="pgaptmp_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGU4471399.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         2782 a         3046 c         2811 g         2654 t
ORIGIN      
        1 ctcgcagaaa gtccacacca acgttgtaaa ttaaccctgt gctagttgga atgatgagaa
       61 attgctatca gtattgtcat tttcagaaaa tatttatcct gcatcggtga gtcagagcaa
      121 gatcagactt ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt caccgtaccg
      181 ctctgtgacg tactatagtc ggcaaacgtc tcaccttgag gttaaaaaat gagcaacatc
      241 ctgattatca acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa cgacaccctg
      301 accgaagtcg cggatggcac actgcgcgac ctcgggcatg atgttcgcat cgttcgcgcc
      361 gacagcgact acgatgtcaa agcggaagta caaaactttc tctgggctga tgtggtgatc
      421 tggcagatgc caggctggtg gatgggcgcg ccgtggacag tgaaaaaata cattgatgat
      481 gtattcaccg aaggtcacgg gactctgtat gccagcgatg gtcgtacccg caaagatccg
      541 tcgaaaaaat acggttccgg cggcctggta cagggcaaaa aatatatgct ttctctgacc
      601 tggaacgcgc caatggaagc cttcaccgaa aaagatcagt tcttccacgg cgttggcgtt
      661 gacggtgtgt atctgccgtt ccataaagca aaccaattcc tcggtatgga accgctgccg
      721 acatttatcg ctaatgacgt gataaaaatg cctgatgttc cccgctatac tgaagaatat
      781 cgcaagcatc ttgtggaaat ttttggttaa ctagagctca ggctttagaa ggagttaacc
      841 atgcttaccg taatcgcaga aatccgtact cgtcctggtc aacatcaccg tcaggcggta
      901 ttggatcagt ttgctaaaat cgttccaacc gtactgaaag aagaaggttg ccacggctat
      961 gcgccaatgg tggattgcgc tgctggcgtg agtttccagt ctatggcacc ggattctatc
     1021 gtgatgattg agcagtggga aagcatcgcg catcttgaag cgcatctgca aaccccgcac
     1081 atgaaggcgt atagcgaagc cgtaaaaggt gacgtgctgg agatgaatat tcgtattctg
     1141 caaccgggga tttaaacctg ccttgtttgc ccggccatcc tgaccgggca atgttctttc
     1201 ctttaaacct caatctccgc catgtcgcct ttctcttgca accaattgcg gcgatcttcc
     1261 gagcgtttct tcgccagcag catatccatc atcgcgtcag tacgctgatc gtcttcatca
     1321 tcgatagtca gctgcaccag acggcgagtg ttcggatcaa gcgtggtttc gcgcaattgc
     1381 atcgggttca tttcccccag acctttaaaa cgctggacgt tcggcttgcc tttcttgcgt
     1441 tttaattgct caagtacgcc ctctttctct tcttccgtca gcgcgtaata aacctctttc
     1501 ccgagatcaa tacggtagag cggtggcagt gcgacgtaaa cgtgaccgtg tttcaccaac
     1561 gcgcggaagt gttttacgaa caaagcgcag agcagcgtgg caatgtgcag accatcagag
     1621 tccgcatccg cgaggataca gattttgcca taacgaagct ggctcaggtc gtcgctgtca
     1681 ggatcgatac cgatcgctac cgaaatatca tgcacttcct gcgaggccag cacttcgtcg
     1741 gaagaaactt cccaggtgtt aaggatctta cctttcagcg gcatgatcgc ctgatattca
     1801 cgatcgcgcg cctgcttggc agatccgcct gcggagtcac cttccacaag gaacagttcg
     1861 gtacggttaa gatcctgcgc ggtacagtca gccagtttgc caggcagcgc cgggccgctg
     1921 gtcagctttt tacgcaccac ttttttagcc gcacgcatac gacgctgggc gctggaaatc
     1981 gccatctctg ccagcaactc cgccgcctga acgttctggt tcagccacag gatgaaggca
     2041 tctttcacca cgcccgaaac gaatgccgca cactgacgcg aagagagacg ctctttggtc
     2101 tgcccggcaa actgcggatc ctgcattttt actgacagca cataggcgca gcgatcccag
     2161 atatcttccg ccgacaactt tacaccgcgc ggcaaaatgt tgcggtattc acagaactca
     2221 cgcatcgcgt ccagcaggcc ctgacgcagg ccattaacat gggtaccgcc ctgcatcgtt
     2281 gggatcaggt tgacgtagct ttcggtcagc agttcaccgc cttccggcag ccacagtagc
     2341 gcccagtcca ccgcttcagt atcgccagcg aaattaccga taaagggttt ttctggcagc
     2401 gtcggtaaac cgtttaccgc ttccgccagg taatcattca gaccgtcctg atagcaccag
     2461 cgctgttcgg tgttgttgat ctcatcttta aaggtaatct caacgccggg gcacagtact
     2521 gctttggctt tcagcacatg cgtcaggcgt gaaacagaaa aacgcgggct atcgaagaag
     2581 gtttcatccg gccagaaatg gacgctggtg ccggtattgc gtttaccgca agtgccgaca
     2641 acctgtaaat cctgcacctt ttcgccattt tcaaaggcga tgttataaac ctgaccatcg
     2701 cggcgcacgt taacttctac gcgcttcgac agggcgttaa ccaccgaaat ccccacgcca
     2761 tgcaggccgc cagagaactg gtaatttttg ttagagaatt taccgcctgc atgcagacgg
     2821 caaagaatca gttcaaccgc cggtacaccc tcttccgggt gaatatccac cggcatcccg
     2881 cgcccatcgt caataacttc taacgactgg tcagcatgta aaataacgtc cacgcgtttt
     2941 gcgtgacccg ccagtgcttc atccacactg ttatcaatga cttcttgccc caaatggtta
     3001 gggcgagtgg tatcggtata catccccgga cggcggcgaa ccggctcaag cccggtgagt
     3061 acctcaatgg catcagcgtt ataagtttgc gtcatggttt aagttagcaa ttcgagttga
     3121 tcgtcagaga tggtgcagac caagaaaatc gacgatcggg ttgaaataat cttcgaagcc
     3181 cgtgaatgcg tggttgccgc cttctatgac agtctggcgg caggaagcgt agtacgccac
     3241 cgcctggcgg taatccagca cttcatctcc cgtctgttgc agcagccaga tcaaatccgg
     3301 cgcttccagc gggtcaatct gcatgacttt aagatcgtaa atatggcgtg actctagcac
     3361 atattgctgc ccggtgtagg ggttctcgtt ctgaccgaga tagtccgtca gcagttcaaa
     3421 cgggcgcacc gccgggttta ccaccactgc gggcagcata aaacattgtg acaaccaggt
     3481 ggcgtaatat ccccccagtg acgaaccgac aatacccagc gaatcgccgc catgttccag
     3541 gacaatggat tccagcagct ctgccgcgtc ggaaggatac ggcggcaact gcggaatgat
     3601 catctcaacg tcagggtgat gttccgccag ccagtttttt aacaagctcg cttttgcaga
     3661 gcgcggcgag ctgttgaaac cgtgtaaata aagaagcgta gacatcagta gccttctgaa
     3721 gcggtatcag gttggaaacg tgtgtccgcc aggcgatgca cctcggtggt cagcgtgcca
     3781 tcagcatgta actcgagagt acgccagccg ggcgcgatag tatccagcgt aaagttggaa
     3841 cagtgcggct taaactgcac acaggtcgac ggcgttgcca gcaggcggcg accgttccag
     3901 tcgagatcca gctcctgatg aatatgaccg cacagcaagt atttgacgtg cggaaacttc
     3961 gccagcacgg tatccagttc gcccgcgtta cgcaggctgt gttgatcaag ccaactacaa
     4021 cccgcaggta gcggatgatg atgcagcagc agcaacgtat ggcgctctgg cgcatcggcc
     4081 agtttacgtt ccagccactc aagctgaaac tcgcttagct caccgtgcgg cacgccaaac
     4141 acctggctat ccagcaacag gatttgccat tgctcaccaa taaacacgcg cttcgccggg
     4201 gagatacccg catcctgtaa cgcgctgtac atcgcgggct ggaaatcgtg gttgcccggc
     4261 agccagacgc agggcgcacg aaaacttgcg atgccttcag cgaaatgctg ataggccgca
     4321 gaggattgat cctgcgctaa atcacctgtc gcgacaatca ggtcgaattc gtgctggtgt
     4381 ggacgaatcg cctccagcac cgcctggtag ctttcccagg tgtttacccc taacagggct
     4441 tcgtgctttt gtgcaaacag gtgagtatcg gtaatttgta aaatcctgac tctggcctca
     4501 ccagccagag gaagggttaa caggctttcc aaatggtgtc cttaggtttc acgacgctag
     4561 taaaccggaa tcgccatcgc tccatgtgct aaacagtatc gcaaccagtc cgctaaaaac
     4621 tggttaattt gatgcttttc gtcgcgttga tgcaactttt tattaggata atcataccgc
     4681 gctttgaagc gaaaaatctg ctggcttgaa cacacttcag ccaccatcgc gtcatgatac
     4741 agacgcaccg tcattgacgg aaggctccag taactgatcg cgggcgcagt ctgttctatt
     4801 gtcaccaggg tagtgtatcg ggtcgattcc acaatcgtca gccgatattg tgcgtttgcc
     4861 acctgatagc ttacagtttc gccgggtgcg tcattgcgcg gtaacaaacg gcgcaattgt
     4921 gaaaagttca tctcgcacag gcgcatcatt tcaggaaagt caggtgtgta acgcttcatt
     4981 tatacccact cattttttaa cgcttgatga tgcagctgca gccattgcaa agcgatgacc
     5041 gacgctgcgt tgtcgatttt cccctcttct acccactggt atgcctgttc ccggcttacc
     5101 acatgaacgc gaatatcttc gttttcatca gccagaccgt gaataccgct tgcggtcgtg
     5161 gcgtccactt cgcccaccat aattgacgaa cgctcactgg tgccccccgg gcttgccagg
     5221 aaacttaaca ccggtttggt ccgtttgact atcagccccg cctcttcaat cgcttcgcga
     5281 cgggcaacat cttccacact ttcaccctct tctatcatcc cggcaaccat ctccagtagc
     5341 cagggggttt cgctagtgtc gtacgcggca attcgaatct gctcaatcag cacaacttca
     5401 tcacgcactg ggtcaaaggg tagcaagact gcggcgtgac cgcgctcaaa aatttcccgc
     5461 cgtacctcat gactcatttg cccgttgaat agacgatgac gaaatctata aagatctaat
     5521 gaaaaaaagc cgcgataaag tgtttctcgt gcaataattt ctacatcgtt tttgccaaat
     5581 gtaacgggca ggttgtctgg cttaagcatt gttaatgtcc tggcactaat agtgaattaa
     5641 atgtgaattt cagcgacgtt tgactgccgt ttgagcagtc atgtgttaaa ttgaggcaca
     5701 ttaacgccct atggcacgta acgccaacct tttgcggtag cggcttctgc tagaatccgc
     5761 aataatttta cagtttgatc gcgctaaata ctgcttcacc acaaggaatg caaatgaaga
     5821 aattgctccc cattcttatc ggcctgagcc tttctgggtt cagttcgttg agccaggccg
     5881 agaacctgat gcaagtttat cagcaagcac gccttagtaa cccggaattg cgtaagtctg
     5941 ccgccgatcg tgatgctgcc tttgaaaaaa ttaatgaagc gcgcagtcca ttactgccac
     6001 agctaggttt aggtgcagat tacacctata gcaacggcta ccgcgacgcg aacggcatca
     6061 actctaacgc gaccagtgcg tccctgcagt taactcaatc catttttgat atgtcgaaat
     6121 ggcgtgcgtt aacgctgcag gaaaaagcag cagggattca ggacgtcaca tatcagaccg
     6181 atcagcaaac cttgatcctc aacaccgcga ccgcttattt caacgtgttg aatgctattg
     6241 acgttctttc ctatacacag gcgcaaaaag aagcgatcta ccgtcaatta gatcaaacca
     6301 cccaacgttt taacgtgggc ctggtagcga tcaccgacgt gcagaacgcc cgcgcgcagt
     6361 acgataccgt gctggcgaac gaagtgaccg cacgtaataa ccttgataac gcggtagagc
     6421 agctgcgcca gatcaccggt aactactatc cggaactggc ggcgctgaat gtcgaaaact
     6481 ttaaaaccga caaaccacag ccggttaacg cgctgctgaa agaagccgaa aaacgcaacc
     6541 tgtcgctgtt acaggcacgc ttgagccagg acctggcgcg cgagcaaatt cgccaggcgc
     6601 aggatggtca cttaccgact ctggatttaa cggcttctac cgggatttct gacacctctt
     6661 atagcggttc gaaaacccgt ggtgccgctg gtacccagta tgacgatagc aatatgggcc
     6721 agaacaaagt tggcctgagc ttctcgctgc cgatttatca gggcggaatg gttaactcgc
     6781 aggtgaaaca ggcccagtac aactttgttg gtgccagcga gcaactggaa agcgcgcatc
     6841 gtagcgtcgt gcaaaccgta cgttcctcct tcaacaacat taatgcttct atcagtagta
     6901 ttaacgccta caaacaagcc gtggtttccg cccaaagctc attagacgcg atggaagcgg
     6961 gctactcggt cggtacgcgt accattgttg atgtgttgga tgcgaccacc acgctgtaca
     7021 acgccaagca agagctggcg aatgcgcgtt ataactacct gattaatcag ctgaatatta
     7081 agtcagccct gggtacattg aacgagcagg atctgctggc actgaacaat gcgctgagca
     7141 aaccggtttc cactaatccg gaaaacgttg ccccgcaaac gccggaacag aatgctattg
     7201 ctgatggtta tgcgcctgat agccctgcac ccgtcgttca gcaaacatcc gcacgcacta
     7261 ccaccagtaa cggtcataac cctttccgta actgatgacg acgacggggc ttcggccccg
     7321 tctgaacgta aggcaacgta aagatacggg ttatctgccg cattcttccc ccttctcgct
     7381 tcaatttcga ccagccatcc tctattctga tgggtattta ccactggtcc cggaagacaa
     7441 aaatgaaacg gacaaaatcc atacgccacg catcgttccg caaaaactgg agcgcacgcc
     7501 atctgacacc agtcgctctc gcagttgcca ctgtttttat gctggctggc tgtgaaaaga
     7561 gtgatgaaac agtgtctctc tatcaaaatg ctgacgactg ttcagctgca aacccaggca
     7621 aaagcgccga atgtaccacc gcgtacaaca atgcgctgaa agaagccgaa cgtactgcgc
     7681 cgaaatacgc cacccgtgaa gactgtgttg ctgaatttgg tgaaggtcag tgccagcagg
     7741 caccagccca ggctggcatg gcaccagaaa accaggcgca ggcccagcaa tccagcggga
     7801 gtttctggat gccgctgatg gccggttaca tgatgggtcg tctgatgggc ggcggcgcgg
     7861 gatttgcaca gcagccgctg ttctcctcga aaaacccagc cagcccggct tacggtaaat
     7921 ataccgacgc gacgggtaaa aactatggcg cagcccagcc aggccgcacc atgaccgtac
     7981 cgaagacagc catggcacca aaaccggcga ccaccactac cgttacccgt ggcggttttg
     8041 gtgaatctgt tgccaaacaa agcactatgc agcgtagcgc aaccggtacc tcttctcgtt
     8101 caatgggtgg ctgataccga tggaaagagt cagtattacc gagcgcccgg actggcgtga
     8161 gaaagcccac gaatacggtt tcaattttca caccatgtac ggcgagccgt actggtgtga
     8221 agatgcttac tacaagttga ccctcgccca ggttgaaaag ctggaagaag tcaccgccga
     8281 actgcaccag atgtgcctga aagtggtgga aaaagtgatc gccagcgatg agctgatgac
     8341 caaattccgc attccaaaac acacctggag ttttgtgcgc cagtcatggc tgacgcacca
     8401 gccatcgctt tattcgcgtc ttgatctggc gtgggatggc actggcgaac ctaaacttct
     8461 ggaaaataac gccgatacgc caacgtcact atacgaggcg gcgttctttc agtggatctg
     8521 gctggaagat cagcttaacg ccggtaactt gccggagggc agcgaccagt ttaacagtct
     8581 gcaagaaaaa ctgatcgatc gcttcgttga gctgcgtgaa cagtatggct tccagttgct
     8641 gcatctcacc tgctgtcgcg acacggtgga agatcgcggt accattcagt atttgcagga
     8701 ctgcgcgacg gaagctgaaa tcgctaccga gttcctctac attgacgata tcgggttagg
     8761 tgaaaaaggt cagttcacgg atttacagga tcaggtaatt tccaacctgt tcaagctgta
     8821 tccgtgggaa tttatgttgc gtgagatgtt ttccaccaag ctggaggatg caggcgtacg
     8881 ctggctggaa ccggcgtgga agagcattat ctccaacaag gcacttctac cgctactgtg
     8941 ggagatgttc ccgaatcacc cgaacctgct gcccgcttat tttgcggaag atgatcatcc
     9001 gcaaatggaa aaatatgtgg ttaaaccgat cttctcccgt gaaggcgcaa acgtgtcgat
     9061 cattgagaac ggcaaaacca ttgaagcagc ggaaggtccg tatggcgaag aagggatgat
     9121 tgttcagcaa ttccacccgt taccgaaatt cggcgacagc tatatgctga ttggtagctg
     9181 gctggtgaac gatcaacccg ccggaattgg cattcgtgaa gaccgtgcat tgatcaccca
     9241 ggatatgtct cggttttatc cacatatttt tgttgaataa tcaatgatgc cggatggcat
     9301 tcgccatccg gtaattgtta gcctatctgc accgacagca tacttaaact gcccatttct
     9361 ataccatcga ccggaatggt aattggctcc tgcccatccc acgcacctaa cacatacaac
     9421 aacggcagat agtgctctgg cgttgggttc gataacgcgc caccttcatg gtcgagataa
     9481 ttcaccagag gatgctgctc tactggccct tgccacgtca gattcgcttt cacatactca
     9541 ttaaacgacg tcgcccacgg atacggtgaa ctatcaccgt gccacttcac tgtacgcagg
     9601 ttatgcacca cgttgccgct ggcgaccaac attattcctt catcacgcag cgccgcgagt
     9661 tttcgcccca tttcgaaatg ccaggcggca ggtttgctac tatcgatact caactgcacc
     9721 atcgggatat cggcgtcagg atacatctta atcagcacgc cccaggaacc gtggtcaaag
     9781 ccccaggctt ctttatccag cgtcaccggg accggcgcta acagctcaac cagatgctgt
     9841 gccagcgcag gcgaacccgg agcaggataa tgcgtatcgt acagcgcctg cgggaagcca
     9901 ccaaagtcat gaatcgtggg cggcgtctcc atcgcggtca ctcctgttcc acgggtaaac
     9961 cagtgagccg aaactaccac aatcgcttgc gggcgtggca atgtcatccc caacgtctgc
    10021 cagctgcggg tatacaaatt atcttccagc acgttcatcg gactaccgtg acctaaaaac
    10081 aatgctggca tacgtgttga agacatgatg atatccttaa ctaaaggtgt cattttgata
    10141 tcctcacaat acgcttgttc ggcggagtaa gaacccggat aacaatgatg atgatcatca
    10201 gttattttga cgatctgcct gaaggtgaag atttataagg agttgtcgat gtcagtacct
    10261 ctcattctga ccatactggc gggggcagcc acgtttattg gcgcgtttct cggcgttctc
    10321 gggcaaaaac cctcgaaccg cttactggcg ttttcgctag gatttgcggc ggggatcatg
    10381 ttgctcatct cattaatgga aatgcttcct gccgcactag cagctgaagg aatgtcgcct
    10441 gtgttgggtt atggaatgtt tatcttcggt ctgcttggct attttggcct ggaccgcatg
    10501 ttgccacatg cgcatccgca ggatttaatg caaaaatcgg tgcagccgtt gccaaaatcg
    10561 atcaagcgca cagccattct gctcactctc ggcatcagtc tgcataactt cccggaaggg
    10621 attgccacct ttgtcacggc gagcagcaac ctggagctgg gatttggcat cgctctggcc
    10681 gtagcgttgc acaatattcc tgaaggtctg gcagtggcag gcccggttta cgcagcaacg
    10741 gggtctaaac gcacggcgat tctgtgggcg gggatttctg gactagcaga aattcttggt
    10801 ggcgtgctgg cgtggttaat cctcggtagc atgatttccc cggtggtcat ggcagcaatc
    10861 atggcggcgg tcgcaggaat tatggtggcg ctctcggttg atgaattaat gccgctcgcc
    10921 aaagagattg accctaataa taaccccagc tatggcgtac tatgtggaat gtcagtgatg
    10981 ggattcagtt tggtgctgct acaaacggcg ggaattggtt aaaaacaaca tcagaagttg
    11041 tccagcgcat ggacaacttc tgctagtttc actaaagcct tattctacct tttcggctgt
    11101 ctcttctctc gtactgttta agtatttgtt ccgcgcttca agcagtgcgt ctctgtcctg
    11161 gcgaaatgcc cgacaataac gtttcaaatg caggaaataa cccaccacaa ccagaacaat
    11221 caatacaatc cagtaccagg cgataaacat ccttttaccc ttaatattta acaatactaa
    11281 acatttagcg cat
//
LOCUS       DAZKFU020000146        11276 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZKFU020000146 DAZKFU020000000
VERSION     DAZKFU020000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.147"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            186..1052
                     /gene="folD"
                     /locus_tag="ACHOQQ_RS15935"
                     /old_locus_tag="pgaptmp_003187"
     CDS             186..1052
                     /gene="folD"
                     /locus_tag="ACHOQQ_RS15935"
                     /old_locus_tag="pgaptmp_003187"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGU4471400.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1054..1266
                     /gene="ybcJ"
                     /locus_tag="ACHOQQ_RS15940"
                     /old_locus_tag="pgaptmp_003188"
     CDS             1054..1266
                     /gene="ybcJ"
                     /locus_tag="ACHOQQ_RS15940"
                     /old_locus_tag="pgaptmp_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGU4471401.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1374..1895
                     /locus_tag="ACHOQQ_RS15945"
                     /old_locus_tag="pgaptmp_003189"
     CDS             1374..1895
                     /locus_tag="ACHOQQ_RS15945"
                     /old_locus_tag="pgaptmp_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4471402.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1931..3316)
                     /gene="cysS"
                     /locus_tag="ACHOQQ_RS15950"
                     /old_locus_tag="pgaptmp_003190"
     CDS             complement(1931..3316)
                     /gene="cysS"
                     /locus_tag="ACHOQQ_RS15950"
                     /old_locus_tag="pgaptmp_003190"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGU4471403.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3490..3984
                     /gene="ppiB"
                     /locus_tag="ACHOQQ_RS15955"
                     /old_locus_tag="pgaptmp_003191"
     CDS             3490..3984
                     /gene="ppiB"
                     /locus_tag="ACHOQQ_RS15955"
                     /old_locus_tag="pgaptmp_003191"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGU4471404.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3987..4709
                     /gene="lpxH"
                     /locus_tag="ACHOQQ_RS15960"
                     /old_locus_tag="pgaptmp_003192"
     CDS             3987..4709
                     /gene="lpxH"
                     /locus_tag="ACHOQQ_RS15960"
                     /old_locus_tag="pgaptmp_003192"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGU4471405.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARNADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4827..5336
                     /gene="purE"
                     /locus_tag="ACHOQQ_RS15965"
                     /old_locus_tag="pgaptmp_003193"
     CDS             4827..5336
                     /gene="purE"
                     /locus_tag="ACHOQQ_RS15965"
                     /old_locus_tag="pgaptmp_003193"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGU4471406.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5333..6400
                     /gene="purK"
                     /locus_tag="ACHOQQ_RS15970"
                     /old_locus_tag="pgaptmp_003194"
     CDS             5333..6400
                     /gene="purK"
                     /locus_tag="ACHOQQ_RS15970"
                     /old_locus_tag="pgaptmp_003194"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGU4471407.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6539..7432)
                     /locus_tag="ACHOQQ_RS15975"
                     /old_locus_tag="pgaptmp_003195"
     CDS             complement(6539..7432)
                     /locus_tag="ACHOQQ_RS15975"
                     /old_locus_tag="pgaptmp_003195"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4471408.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7429..8244)
                     /locus_tag="ACHOQQ_RS15980"
                     /old_locus_tag="pgaptmp_003196"
     CDS             complement(7429..8244)
                     /locus_tag="ACHOQQ_RS15980"
                     /old_locus_tag="pgaptmp_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4471409.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVTQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8255..9514)
                     /locus_tag="ACHOQQ_RS15985"
                     /old_locus_tag="pgaptmp_003197"
     CDS             complement(8255..9514)
                     /locus_tag="ACHOQQ_RS15985"
                     /old_locus_tag="pgaptmp_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4471410.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNQGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9524..11191)
                     /locus_tag="ACHOQQ_RS15990"
                     /old_locus_tag="pgaptmp_003198"
     CDS             complement(9524..11191)
                     /locus_tag="ACHOQQ_RS15990"
                     /old_locus_tag="pgaptmp_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4471411.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFRHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALCLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGNLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2491 a         3242 c         2990 g         2553 t
ORIGIN      
        1 tttacaacgc catcatggac gaaaaatgaa gctttcaatc tcagcgacct cagcgacggt
       61 gcgcctaacc ttcgcaagag gcgcttcacg cgataaatct gaaacgaaac ctgacagcgc
      121 gccccgcttc tgacaaaata ggcgcatccc cttcgaccta cgtaacagat ggaatcctct
      181 ctctgatggc agcaaagatt attgacggta aaacgattgc gcagcaggtg cgctctgaag
      241 ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg ggcaccagga ctggccgttg
      301 tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc aagcaaacgc aaggcttgtg
      361 aagaagtcgg gttcgtctcc cgctcttatg acctcccgga aaccaccagc gaagcggagc
      421 tgttggagct tatcgatgcg ctgaatgccg acaacaccat cgatggcatt ctggttcaac
      481 tgccgttacc ggcgggtatt gataacgtca aagtactgga acgtatacac ccggacaaag
      541 acgtagacgg tttccatcct tataacgtcg gtcgtctgtg ccagcgtgcg ccgcgtctgc
      601 gtccctgcac cccgcgcggt attgtcacgc tgcttgagcg ttacaacatt gataccttcg
      661 gcctcaacgc cgtggtgatt ggcgcatcga atatcgttgg ccgcccgatg agcatggaac
      721 tgctactggc aggttgcacc accaccgtta cccaccgttt cactaaaaat ctgcgtcacc
      781 acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa gccgggcttt attcctggtg
      841 actggatcaa agaaggcgca attgtgattg atgtcggcat caaccgtctg gaaaatggca
      901 aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg cgcctcatac attacgcctg
      961 ttccgggcgg cgttggcccg atgacggttg ccacgctgat tgaaaacacg ctacaggcgt
     1021 gcgttgaata tcatgatcca cagggtgagt aacatggcga cattttcttt aggtaaacat
     1081 ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct ggagcgaaag cggcgcgcag
     1141 gcgaaaatcg cgattgccga aggccaggtg aaagtcgacg gcgcggttga aacgcgcaaa
     1201 cgctgcaaaa tcgtcgccgg gcagacagtg agttttgcag gtcacagcgt acaggttgtt
     1261 gcctgatccc tttcccggcc tgtatcaaca ggccggttct cttttcagac ttttgccctc
     1321 tacgccccgt tcatccatgc tgatagcatc gacattttgc cgaagcgacc atcatgccaa
     1381 ccgttattac gcacgccgcc gttcccctat gcattggttt agggttgggt tcgaaagtca
     1441 tccctccgcg tctgttattt gccggaatca tcctggcgat gctgcccgat gccgacgtct
     1501 tgtcgtttaa atttggcgtt gcttacggca atgtttttgg tcatcgcggg tttacccatt
     1561 cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct gattggacgt cgatggttca
     1621 gggctgggct gattcgctgc tggctgtttt taaccgtctc gttgctatcg cacagcttgc
     1681 tggattcggt aaccactggc ggtaaagggg ttggctggct gtggccgtgg tcagatgaac
     1741 gctttttcgc tccctggcag gtgattaaag tcgcgccgtt tgcattgtct cgttacacca
     1801 cagcgtacgg gcatcaggtg attatttctg aattgatgtg ggtgtggttg ccggggatgc
     1861 tactgatggg aatgttgtgg tggcgcagac gataaccgga tgcgaaaact cgcatccggc
     1921 aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc
     1981 ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc ccagtctttc gctttacggg
     2041 catccagacg ctgttgaatt aacgcttcaa tctcagccac ttcgctgtca tctgcctgcg
     2101 cgccgctttg caggaacgct tccggttctt gctccagcag gcccaataca gcggaaagtt
     2161 tacgcaggtg agatgccatt gcattcgccg ctgccatatc ttctgctttc agacggttca
     2221 cttcacgcgc catatcaaac agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca
     2281 tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc aggcgcaacg gttttatctg
     2341 tgccgcgcag cgcagtgtag agacgctcca gcgccgcacg cgcctgcttc aggttctctt
     2401 cgctatagtt cagctggctg cgatagtggc ccgacatcag gaagtaacgc acggtttccg
     2461 cgtcgtagta tttcagcaca tcgcgcacgg taaagaagtt acccagcgat ttggacatct
     2521 tctcgcggtc aaccatcacc atccccgagt gcatccagta gttcacatac tggccatcat
     2581 gggcacaggt ggactgcgcg atttcgtttt cgtggtgtgg gaacatcagg tcagaaccgc
     2641 cgccgtggat atcaaagtgg ttacccagct gcttgcagtt cattgccgaa cattcaatgt
     2701 gccagccagg acggcccgcg ccccacggag acggccagct cggttcgccc tctttcgaca
     2761 tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc gaccacgtca acgcgcgcgc
     2821 ctgcctgcag ctggtcgaga tcctgacgcg acagcacgcc ataagttgga tcggtcggga
     2881 cgtcgaacat cacgtcgccg ttgtccgcca cataagcgtg acctttggcg atcagttgtt
     2941 cagtgagttc aataatttct gcgatatggt gcgtcgcgcg cggctccata tccgggcgca
     3001 gaatgttcaa cgcatcaaaa tctttgtgca tttcggcgat catgcgatcc accagcgcca
     3061 caaagctttc gccattttca ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt
     3121 tgcgcacata cttcagctta tagccgagga aacgcagata gcgcgcaacc acgtcaaaag
     3181 caacaaaggt acgcccgtga ccgatatgac agagatcgta aacggtgatt ccacacacgt
     3241 acatgccgac ttccccggcg tgaataggct taaattcctc tttttggcgt gtcagagtat
     3301 tgaagatttt tagcatcgaa gattccgttt agacatgtgt gggtaattga gttgcgtata
     3361 atacccatat ttcccgcgcg aatcagcata cattgcgtga tgatccgatc gtgcggttat
     3421 gctataacac caccctatat atgacccgaa ctgggttgaa gcaccaatca aacggaacag
     3481 gatgcaaaaa tggttacttt ccacaccaat cacggcgata ttgtcatcaa aacttttgac
     3541 gataaagcac ctgaaacagt taaaaacttc ctggactact gccgcgaagg tttttacaac
     3601 aacaccattt tccaccgtgt tatcaacggc tttatgattc agggcggtgg ttttgaaccg
     3661 ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg aagccaacaa cggtctgaaa
     3721 aatacccgtg gtacgctggc aatggcccgt actcaggctc cgcactctgc aactgcacag
     3781 ttcttcatca acgtggttga taacgacttc ctgaacttct ctggcgaaag cctgcaaggt
     3841 tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg acgtggtaga caaaatcaaa
     3901 ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc caaaagaaga cgttatcatt
     3961 gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt tattgcagat cttcatctct
     4021 gcgtggaaga accggcgatc accgccggtt ttctgcgttt tttagcgggg gaagcccgca
     4081 atgccgacgc gctgtatatt cttggcgatc tgtttgaagc gtggattggc gacgacgatc
     4141 ccaacccact ccatcgccag atggcggcag cgatcaaagc ggtgtccgat tccggcgttc
     4201 cctgttattt cattcatggc aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa
     4261 gcggcatgac gttattgccg gaagaaaagg tgctcgaact ttatggtcgt cgggtgttga
     4321 ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag
     4381 tccacaaacc ctggctgcaa acgctattcc tcgccctgcc gttgtttgtg cgcaaacgca
     4441 ttgccgcgcg aatgcgcgcg aacagtaaag aagccaacag cagtaaatcg ctggcgatca
     4501 tggacgttaa ccaaaacgcg gtggtcagtg cgatggaaaa acatcaggtg caatggctga
     4561 tccacgggca tacccatcgc ccggcggtac atgaacttat cgccaaccag caacccgctt
     4621 ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat ggtgaaagtc acggcggatg
     4681 acgttgagct gattcatttt ccgttttaaa aaacccgcaa ctttgctgat ttcacagcca
     4741 cgcaaccgtt ttccttgctc tctttccgtg ctattctctg tgccctctaa agccgagagt
     4801 tgtgcaccac aggagtttta agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca
     4861 tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt cgccgccgaa atcttcgaaa
     4921 tcctgaatgt cccgcaccac gttgaagtgg tttctgctca ccgcaccccc gataaactat
     4981 tcagcttcgc cgaaagtgcc gaagagaacg gttatcaggt gattattgcg ggcgcaggcg
     5041 gcgcagcgca cctgccaggc atgattgccg ccaaaacgct ggtgccagtg ctgggcgtgc
     5101 cagtacagag cgccgcactg agcggtgtcg atagcctcta ctccatcgta caaatgccgc
     5161 gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg cgcggcaaac gcggcgttac
     5221 tggcagcaca aattcttgcg actcatgata aagaactgca ccagcgtctg aatgactggc
     5281 gcaaagccca gaccgacgaa gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca
     5341 ggtttgcgtc ctcgggaacg ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt
     5401 aggcattgct gtctggccag tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca
     5461 aagcgtgatt accgctgaga tcgaacgctg gccggaaacc gcattaaccc gcgagctggc
     5521 gcgccatcct gcctttgtga accgcgatgt gttcccgatt attgctgacc gtctgactca
     5581 gaagcagctt ttcgataagc tccacctgcc gaccgcaccg tggcagttac ttgccgatcg
     5641 cagcgagtgg cctgcggtgt ttgatcgttt aggtgagctg gcgattgtta agcgtcgcac
     5701 tggtggctat gacggacgcg gtcaatggcg tttacgcgcc gatgaaaccg agcagttacc
     5761 ggctgaatgc tacggcgaat gtattgtcga gcagggcata aacttctctg gtgaagtgtc
     5821 gctggttggc gcgcgcggct ttgatggcag caccgtgttt tatccgctga cgcataacct
     5881 gcatcaggac ggtattttgc gcaccagcgt cgctttcccg caggccaatg cacagcagca
     5941 agcgcaagcc gaagagatgc tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat
     6001 ggcgatggag tgttttgtca ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt
     6061 gcataacagc ggtcactgga cacaaaacgg tgccagcatc agccagtttg agctgcatct
     6121 gcgggcgatt accgatctgc cgttaccgca accagtggtg aataatccgt cggtgatgat
     6181 caatctgatt ggtagcgatg tgaattatga ctggctgaaa ctgccgctgg tgcatctgca
     6241 ctggtacgac aaagaagtcc gtccgggccg taaagtgggg catctgaatt tgaccgacag
     6301 cgacacatcg cgtctgtccg cgacgctgga agccttaatc ccgctgctgc cgccggagta
     6361 tgccagcggc gtgatgtggg cacagagtaa gttcagttaa cctggtgctc atttgattgc
     6421 cggatgcggc gtgaacgcct tatccggcct acaccgctcc ggtacccatt gtaggcctga
     6481 taagacgcgt caagcgtcgc atcaggcatg gtgcaccgat tgctggatgc tctcatcact
     6541 acagcgaaat acaggtcccc gcttcgcccg ccagcgtctc ttcaattcgc gataacgccc
     6601 caatccacgc gggtttacca cggcttctga cataaccact caccgccgtt accttcggcc
     6661 ccatcgaacc atcggctttg gcaaatggcg ctaactcatc cggtgtggca tggcgaatgg
     6721 cacgttgctg cggcgttccc cagttttcat ataccgcatc agcatcggtg aggatcacca
     6781 gtccatcggc attaatctgc tcggcgagca acgcagcagc gagatcttta tcaatcaccg
     6841 cttcactccc tgccccgtct tccgtcacag gcacaccgcc gccgccactg caaatcacca
     6901 catgcccctc tttgagcaac aactcgatgg cttcgctgtc gagaatttta cgcggttgcg
     6961 gagacgccac cacccggcgc aaatatttac catcacgttt catctgccag ccgtaagccg
     7021 cttccagtgc ctcttgttct tctggctgat aaaccggacc aataaatttc tctggctgca
     7081 aaaacgccgg atcatcaggc gaaacctcaa tgcgcgtcag caccgtcgtg acaggcggca
     7141 tctgcggctg tgcgctcaaa ctctgcgcca gcatatagcc aatcatcccc tggctttccg
     7201 ccaccagcac atccagcgga tacggttcta cctctttcca cgccagattc tgtaacgcca
     7261 gcagccccac ctgcggcccg ttgccgtgaa caatcgccag ccgataagaa cgggccaggc
     7321 gcgccagcgc gggtacagca ctggcgatat tgcgatattg attttctgcc gtcagcgcct
     7381 caccgcgctg aagcaaggca ttgcccccaa gagctacaac cagtgttttc atggttttcc
     7441 ttgtaataat tgttgaccaa gccagaaccc cagcagtgta tctgcccctg acgtatgccc
     7501 cagcgccagc agcgaatcaa tcgcaacagc ggtacgtttc gggcaactca gagcatgaac
     7561 aaagtgcagg agtggcgagg cgaaatatcc ttgcgcggca taacgtaaat aactgacgct
     7621 caccgctgtg gtaacgagtt gcagattgtc ggaacaggca aaaaacggac gaccggagcg
     7681 cgcatctaaa gcgccataat accaggccgc cagcagcatt ccgctcaacg tgtcatcatg
     7741 acttggcgtt aatccggggc ctttacccag ccagtgccgc cagtcggtct taacgccatt
     7801 gagcgcggcc tgaaaacagt gacgaaactg gcgtaattca gcgggcagcg gatcgcttgc
     7861 cgccatcgcc agtggcccga aaagtccggt ttcctccgcg cgttgcatcc atgcagctgc
     7921 aagcggttga ggatgcgaag gcggcgtaat acgcagcaaa caataacgct gtggctgtgt
     7981 aaccgtgaaa cgcccgaggc gaatcccttg agccacaacc tgtggtcgtt cattgccgca
     8041 taatccaccg cataacgcat cgaattgcgc acggcgaagc acccatcctc cggggccgaa
     8101 accactaccc tgacgatgca gcgttaacag ttcgccgctt tccgtcatca ggttaatcgc
     8161 ccgccgccac actcccgcta accgccagga ctggcgataa ttcggtgcgc tactactggc
     8221 aagcagagga tggatgatcg tcattgcgcg ttcctcaacc aatacccatg ctttcagcca
     8281 gtgcttccag cgcctgttca aagcacgcca gcggtgcccg cacggtgcct gcgccaatct
     8341 gcccgatccc cgcctcttta tgggcgatac cggtattgat gagtggcgta atgccggttt
     8401 ctaccacgcg acgaatgtcc agtcccaggc acgcgccctg aaaatcccag cttgggatct
     8461 gcaactgcat attgcgttca aggtaaattt ccgccatctc ttcagatacc gccctggccg
     8521 cttccatgcc acccgcaccg acaaagcgcg ttacgccagg cgctgcgatc attgccgcac
     8581 ctccgatacc aaaggtttcg gtaatcgcgc tatcgcccat atccgggttc gcctgctcct
     8641 gcgagaagcc ggtgaaaaac agaccttgcg gagtgtttac aggcgcagta aaccagcgtt
     8701 cgcccagccc gcttacccga atcccgaaca tattgccgtt gcgggtcatt gccgtgacga
     8761 tgctgcctgc gcggatcatc gcgccagcat ccatcgccgc cttgcagtaa gccatcgcga
     8821 ggttgaggaa gaactgatcg gtcacgctga ggaaatccat cacttccgcg atgtgctgtt
     8881 tatcatgatc gaggcgagca atttgtgggg ccagcgcacg cattaacagt gctgaggaag
     8941 caatattgcg ttgatggaac tcatcgccca tcgtaatgcc ctgcgccatc atcgcggtaa
     9001 gatcgatacc gcgttccatg cgccccagcg ccgcgcttaa tactggcatt aacacatcgc
     9061 gcatccagcg atggcgagtc aggacatctt cgccgtaagc gccaaaacgc atcactttgc
     9121 cgataccttc gttgaggttg cagtacgccc ggttgccgtc ggtcacgttc tcgaccacca
     9181 gcatcggcat actggcagaa gtaataccgc ccattggccc gacggcattc acatggtgac
     9241 aaggaatgaa gttcacttcc ccctgctcca gtattgccag cgcctgcgct tcatctttcg
     9301 cccagccttc gaacagacat gcgcccacgc acgccccttt catgggtccg gtcatctcct
     9361 gccagcgcat tggcggcccg gcgtgaagca gtgttttgcc ctggtttagt tcgctgataa
     9421 gtgaagaagc cggttgcaca tccagccagt gtggacgagc gcgacgaatt tgttcgataa
     9481 ccgcagcatt ggcttgcgcc actgatgtaa acatgggaac cccttattgc aaacgttcta
     9541 ataaacgagc cagtttttta ttgccaccgg cgactggcga ccattggtaa tgcacaaccg
     9601 gtttgctggc gctttgtagc tccagcgcaa aactgcgtaa tccgatgtta atcacggcga
     9661 cgttttccaa taatgacggt gtgtgttgct gtgcggcagg cgagagcgga tgaattaacg
     9721 ctgccgccag caaggtggct tccggtagcg aactcacgac cgcaatcccc gcatcttcca
     9781 gcgtggcgat ttgctgcgag cggcattgcg ggtcacgttc agtgcctgtc accgtggcaa
     9841 tggcatacag tggttgattg tctgaacgcg cagcacaggc tttttgccag gcgcttacca
     9901 gcgaggcggc aggatcggca gtcgcaccga agccaatcac gacatcaagc agcagcacac
     9961 gcacttgcgg tttagcgccg agatcggcaa ttaactggtt gcgtaaggtt gggtcgatca
    10021 tcggatgggg acgcccgacg gtgtagaaat catcgccgag gtcaataatc tggtggccat
    10081 cggcgtccag catcatgccg tgatgatggg catcatcggc ttccacgcca aggtttccgg
    10141 caagtaatcc cgccgcttcg gcagccagcg taccgccggt atacaaaccg caaataaatc
    10201 cgctgctggc aggcgttatc gcgttacgtc gcgccgtaac gcgtgaaagc aggcaagcca
    10261 ggcgtgcggc ctcatccagc gaggaggcaa accagacatt ctcgtcgcgg gccaccgccg
    10321 gggtataacc taaacacagc gccaccgtcg gtttgccggt tgctttcatg gcattaacaa
    10381 ttttcagacg caccgcttcg gcaggtggtt ttgaaacaaa tgccagcact tcgcttttct
    10441 cgtctgcact gagcatttcc agcgccgtca gcgcactgat gccgcccact tcacggctga
    10501 ggtcgcggcc gccaaggcca atcgcgtgag taattccctc ccctgccagc gcaatctgcg
    10561 agcacagctc ctgaatcccg gtaccggaag cgccaatgac gccaatgttg ccttccggca
    10621 tcacgttagc aaaagccagc ggcgtgccgg caatcatcga cgtaccgcag tccggcccca
    10681 tcaccagcaa gcctttttcc cgcgcgcggg ttttaagttg gatttcatct tccagcgtga
    10741 cgttatcgga gaacatcatc acgttgaggt tgcgatccag cgcctgattc gccagctccg
    10801 ccgcatactc gccagccact gaaatcagcg ccagattggc atcgggtaat ttctgacagg
    10861 cactgtccca gcgacgcacc tgcgtcaacg cctggctgct gcctgccccc tgcgccagtt
    10921 gttttagcgc ctcttcaagc tgctgcataa tcgcctgcgc gatccccgca tccgccgctt
    10981 cgctacgaat tgccacgcaa atatcgttcg gcgtggcgtt attaaaatcg tcatgcctga
    11041 aacctgtggt atctaataac gctttattgg cgggcgtacc catcattacg gaaacatcat
    11101 caacattttc tgattcgctg agttttcgtg aaataatcat taaactgacc gaatcctgaa
    11161 aacacccttt tttaataaag gcgtggatca taacaactcc ttaaaaattg cacccatggc
    11221 aattgcacga gcagtgttaa tgtggcgatt acggtatcga tgacaacggg taaata
//
LOCUS       DAZKFU020000147        11150 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZKFU020000147 DAZKFU020000000
VERSION     DAZKFU020000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.148"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..1004
                     /locus_tag="ACHOQQ_RS15995"
                     /old_locus_tag="pgaptmp_003199"
     CDS             99..1004
                     /locus_tag="ACHOQQ_RS15995"
                     /old_locus_tag="pgaptmp_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4471412.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(1038..1640)
                     /gene="cobO"
                     /locus_tag="ACHOQQ_RS16000"
                     /old_locus_tag="pgaptmp_003200"
     CDS             complement(1038..1640)
                     /gene="cobO"
                     /locus_tag="ACHOQQ_RS16000"
                     /old_locus_tag="pgaptmp_003200"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU4471413.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1650..3302)
                     /locus_tag="ACHOQQ_RS16005"
                     /old_locus_tag="pgaptmp_003201"
     CDS             complement(1650..3302)
                     /locus_tag="ACHOQQ_RS16005"
                     /old_locus_tag="pgaptmp_003201"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4471414.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIIASKGQQIWTGGNDAEALSKGIYTTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDICAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKALL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMLDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGESMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3410..4690)
                     /locus_tag="ACHOQQ_RS16010"
                     /old_locus_tag="pgaptmp_003202"
     CDS             complement(3410..4690)
                     /locus_tag="ACHOQQ_RS16010"
                     /old_locus_tag="pgaptmp_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HGU4471415.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4851..5168)
                     /locus_tag="ACHOQQ_RS16015"
                     /old_locus_tag="pgaptmp_003203"
     CDS             complement(4851..5168)
                     /locus_tag="ACHOQQ_RS16015"
                     /old_locus_tag="pgaptmp_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HGU4471416.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5165..6535)
                     /locus_tag="ACHOQQ_RS16020"
                     /old_locus_tag="pgaptmp_003204"
     CDS             complement(5165..6535)
                     /locus_tag="ACHOQQ_RS16020"
                     /old_locus_tag="pgaptmp_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HGU4471417.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AQEEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAEIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6539..7780)
                     /locus_tag="ACHOQQ_RS16025"
                     /old_locus_tag="pgaptmp_003205"
     CDS             complement(6539..7780)
                     /locus_tag="ACHOQQ_RS16025"
                     /old_locus_tag="pgaptmp_003205"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HGU4471418.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     TVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7780..9225)
                     /locus_tag="ACHOQQ_RS16030"
                     /old_locus_tag="pgaptmp_003206"
     CDS             complement(7780..9225)
                     /locus_tag="ACHOQQ_RS16030"
                     /old_locus_tag="pgaptmp_003206"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HGU4471419.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNAGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9244..10632)
                     /gene="glmL"
                     /locus_tag="ACHOQQ_RS16035"
                     /old_locus_tag="pgaptmp_003207"
     CDS             complement(9244..10632)
                     /gene="glmL"
                     /locus_tag="ACHOQQ_RS16035"
                     /old_locus_tag="pgaptmp_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HGU4471420.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10632..11081)
                     /gene="glmS"
                     /locus_tag="ACHOQQ_RS16040"
                     /old_locus_tag="pgaptmp_003208"
     CDS             complement(10632..11081)
                     /gene="glmS"
                     /locus_tag="ACHOQQ_RS16040"
                     /old_locus_tag="pgaptmp_003208"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HGU4471421.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVENKFKEMGFDRVFAPSHDLEEVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         2637 a         2813 c         2810 g         2890 t
ORIGIN      
        1 atgattctta taaattattt attgctgctt tggttaccat tacttcgtaa taagtaatgt
       61 atttttgatg gtttaaacta tttttaaggg acggcattat gcgagggaaa atacctaaaa
      121 cggaactgct ggtgactttt gaggtcgtcg cacgtcatga aagttatacc agagcggcag
      181 aggagttagc gttaacccaa agtgcagtat tcagacaagt aagtgcgctg gaggaatttt
      241 tacgtactcc gttattttca cactctaaaa aacgcatttt tcttaatgat gcagggaagt
      301 attacctggg gatagtaaaa gaaacattaa ataagctgga acgcgatacc aataccatta
      361 tgacctggca gcctacggtt caggttattg aacttgccgt taatcctact tttagtaccc
      421 actggttgat cccaaattta catgaattca caaagttaca tcccgatatt attgttaata
      481 ttcattcttt agctaataac ggcgattttc ttaatcggga gtacgatgct gtcattatgc
      541 gcgaaaattt ttgcgcgccc tgggcggaag tagaatatct ctttgatgag gagattttgc
      601 cggtatgcag cggcagtttg cttgcaatgt ccgatcaaaa gctgtcagtg gcagaactgc
      661 ttactgagtt accgcttctg catcaaagca cccgtattac tggctgggaa gagtggtttg
      721 ctttatctgg cgttagcagt ccgttggtca ataacggacc acgtttcgat ttgctttcaa
      781 tgctgattgc cgccgtgcgc tctaatctcg gcgtggcgct gttaccacgt ttcgctattc
      841 aacatgattt ggatagtggc gatatggtga tcccctgtga tgtgcctatt cgtaccggta
      901 atcgttttat tatgacgtgg caggaagaga agtctgactc accgcacttg cagcaatttc
      961 gtgaatggtt gctggcgaaa tcggtcgtac cgcaggagat gtaaaacctg cgcctggcgg
     1021 gatgccaggc gcagttatta ccagtcgata cccggctggg cctgtatgcc gttatcaaaa
     1081 gcatgtttga cggggcggat ttcgctgacc gtatctgcca tttttaatat ctgactgtgg
     1141 caaccgcgtc cggtgacaat gacactttgt tgtgccgggc ggttttgcag agaagcgatc
     1201 acttcttcgg tatccagata atggtaagcc agcatatagg tcagctcatc aagcaccact
     1261 aagtcgtaac gcttgtcagc cagcatccgt ttgctttccg accagacctc tttcgccgcg
     1321 tcgatatccg cttgccggtt ctgtgtctcc caggtaaaac cggttcccat aatatgaaac
     1381 tcaaccccca gcggctggag aaggttgtat tcgccgttgt cccattgccc cttgatgtac
     1441 tgcgcaaccc ccaccgtttt gccatgaccc accgcgcgcg tcacagtacc aaacgcggca
     1501 gtggatttcc ctttgccgtt gccggtgaaa acaatcaaga tccctttttt ctctatcgcc
     1561 gctgcgacgc gcgtatcgac ctgttccttc aatttctgtt ggcgctgcac atgccgttca
     1621 gttgaaattc gcgcttccat tcattttcct taatgtcccg cagggcaact ggcgcagcgg
     1681 tgttgctgct caaactgact aaagaaatta ttgcctttat catctaccag gatgaatgcg
     1741 ggcagatttt ccacttccat catccacacc gcttccatac ccagctccgg gtattccagg
     1801 caacgcagac ttttgacata ttcttgcgcc agtaacgcag ctgcgccgcc aatacttccc
     1861 agattgaagc cgccatgttt gtgacaggca tcggtgacct gctggctgcg gttacctttc
     1921 gataacatca ccagactgcc gccggccgcc tggaaggtat cgacataacc gtccattcga
     1981 ccgcccgtgg ttggacccag tgaaccgcag gccatgttct ccggtgtttt cgccgggcca
     2041 gcgtaataga caatatgatg cttcaggtac tccggcatcg attcgccgct atccagccgc
     2101 gcttttattt tggcgtgggc gatatcgcgc gccactacga tgggaccact taacgacacg
     2161 cgggtgccta ccggtaatct ggcgagatcc agcatcacgt cacgcaacgg acggtttaga
     2221 tccagttgta catgttgcgc gtgattttct tcccgcaatg aggctggaat atattgcccc
     2281 ggattgtgtt cgagtttttc cagccagata ccgtgtttgt taatttttgc tttgatattg
     2341 cgatcggcag aacaggagag cgccatagct atcgggcaag agccaccgtg acgcggcaga
     2401 cgaatgacgc gaatatcatg agcgaaatat ttccccccaa attgtgcacc aataccaaat
     2461 tgctgactgg cctcgagcaa cgctttttcc agctcaatat cacggaatgc ttgtccctgc
     2521 tcgtttccgc tggtgggcaa attgtcgtaa tatttcgttg aggccagctt ggcaattttt
     2581 aaggtttgat cggctgaaag ccctccgacg acaaaggcga tgtggtaagg cgggcaggca
     2641 gcggtgccca gcgatttcat tttttcgatt aaaaatgcgg tcagtttttc tggttgcaat
     2701 aaggatttgg tttcctgata aagcgcggct ttattggcag agccgcctcc tttattaaca
     2761 cagaggaaat ggtactcatc accagcgacg gcgcagatat caatctgcgc cgggagattg
     2821 gtttgcgtat tgacttcggt atacatatcc agcggcgcat tttgtgaaaa gcgcagatta
     2881 ttttcctgaa aagtggtgta aatgcctttg cttaaggctt cggcgtcgtt acctccggtc
     2941 catatctgtt gccctttact ggcaataata gttgcggtgc cggtatcctg acagttgggt
     3001 aatacccctt ttgctgagac ttcagcatta cgtaataact gcaacgcaac gtatttatca
     3061 ttactactgg cctgcggatc gttaagaatg ctggcgacct gttgcaaatg agcggaacgt
     3121 aagaaaaaag aggcttcata aaatgcctgc cgggcaagca gcgttaatgc ttcaggggcg
     3181 atttttatca cttcttcgcc gtctaattcc gttactgtaa tatgctgatc gctaattaat
     3241 gtatattctg taccgtcttt attttgtaga aacggttctt gccagataaa tggtttggac
     3301 atatttcact tttcacctta aacgttagtg ttacttaatt gccgtctaac ttcatgatcc
     3361 ggcttataaa aatgcgtagt cattggcttc agattgctta agagccccct cagggcagag
     3421 caacgttcga ttcattgggt tttaatgctt taccccgaca gccctcgccg aaatgttcgc
     3481 cttcccaccg actcaccatc gcagcgccca ttgcgttgct catgacgttt gtcgcggaac
     3541 gccccatatc aaggaacgga tcaacgccca tcagcaagat cagccccgct tccggaatat
     3601 tgaactggtt gagcgtggcg gcgataacca ccattgaggc gcgtggtacg ccagccattc
     3661 ctttcgaggt caacatcagg atcaacagca tggtgatttg ctcaccgatg gataaatgga
     3721 tattgcaggc ctgggcgatg aaaactgtgg cgaaggagca gtaggccatt gatccaacga
     3781 gattaaatga gtagccaatg ggtaagacaa agctggcaat tttgggggaa acgccaaatt
     3841 gctccagttt ttcaagcgtt cccggaaaag ccgcttcaga actggatgtg gtaaatgcca
     3901 gcagggcggg ttccgaaagg gcgcgggtca ggcgtctgat gcaggggccg acataaacga
     3961 tggccagacc gataagcagc acccaaagta ataacatggt gaaataaaat tcacccataa
     4021 agatcccggc gctcaccata actgccagtc ctcgttcagc aatcaatgct gaaatagcag
     4081 cgaatacggt caggggagcg aagagcatga cgtagccagt gagctttaac atggcatgtg
     4141 ccagcgaatc taaggcgtga acgatggcgc tgcctttctc gccaatcgcc gtcaggctac
     4201 agccgaggaa aattgagaac accacgattt gcaaaatttc attgtgcgcc atggcatcga
     4261 caatgctggt ggggaaagca tgcgaaataa atacttttag cgtgaagggt tctgccgcga
     4321 ccactccggt ggtttcggca ccgtgtgcaa caaaattaat tcctgtacct ggcatgaaga
     4381 aatttactgt tatcaagcct aaggcaattg acagcaatga ggcgcaaata aataaaaaga
     4441 gtgttttaga aaaaatacga ccaagggctt tggcatcgcc cattttagca atacctacca
     4501 ccagggtaga aaccactaac ggagcgataa tcatttttat cagtcgtaag aaaatcgtcg
     4561 tgaatatcga tatttcttgc gcatagagtt ttgctgtttc cggtgaagca gtgttattga
     4621 gcactacgcc gataatcatt ccaagtacca gcgccaaaag aatcatcgtg gttaaactta
     4681 ttttcttcat tttttcctcc atcaggtgca cggcgataaa tcagaatcag gagaagtaga
     4741 tccctgaata gagattcagg catctacctg ctattacaac cttacaaata gctgaagtgt
     4801 tgaactgcaa taagtgacgg gatgtcagtt ggctaaaagt attcggattt ttacaaatcg
     4861 aaactcatat ttaataaagg tgcatgttgc tgtgcaccat agacatcatt gtcacctaca
     4921 ttcccggaga taatatctcg tggcatgacg atttttactg cgttagaagg atcaaaccag
     4981 acaatacgat gaataaagtc aggttcaaca tgatataagc gcgcaatcag ttctggggtt
     5041 aattgccctg atgatttaac tcgctcatag ttttctttcg ttttaaataa aatatctaaa
     5101 accagttcgt aaggtccggc atttttcgaa cgaatgactt gcgccagtga gacaattgac
     5161 tgtttcatgc ctgaactcct tctggcgtca cctgttcaat atggaaatca aaacgcagag
     5221 cgtcgttggc ttcaatcagg tgatatatgg aaaattcgta taccggcccg ccctggatat
     5281 cagaagggga gaacgggaac gccagattgc ctgcggtagc gatgcggttt tcatagccgt
     5341 agtgcagcat ggtagagcgc accagcgagc aaacgctatt ggcaatttcc tgggtcggtg
     5401 caactacatc aaggacaatc cccagctcat gcccggcagt ttgcatcggt tcatggtctc
     5461 ccatcacccc gtttttgccg tacaggtgga aattgatgcg aatgctgtca tcgtcgagcg
     5521 acaggttacg cgaaacgctg gttttgactt catcgatgat tttatcgatc tcggcgatca
     5581 taatcgggtc gcgcgttccg gcgatggtca gacagcggaa gcccacccgt cgtgcacctt
     5641 ccagtttcag ggcatacggc gtttcttcat gcttagaacc gctgacgtag acttcgccgt
     5701 cattgactgc tttaaatgtg cagcctttca ggttcaacac gccgccaggt cctggcagga
     5761 agtagggatc ggatttctca tacagtgtgt gtgcagctgc tgacgtttcg gtaaatttac
     5821 gcttcggatt aaatgtcttc agcgtaaagc cgttgtcatc aatgatgccc atcgcacagt
     5881 cagagcctga gccgggcgtt gcggcgattg ccgcgcattc aaggattttc ccacaatgca
     5941 gcgccagacc ttcatcaaag ccttgcatga tcgggagcgc agcaaagcag gccgggtcgt
     6001 aagcgcgccc gcccagcacc acttgcgcgc cttctttcag cgcccgctgg aagggttcga
     6061 tgcccatttg cgcgacgata tacgtacttt cgtcaatcgc gtcgtgggtt aacggcggga
     6121 caaaatccag cgccgtgatt ttgccgttat ccagcgcctg gtggacgatg gctttgtcaa
     6181 cgtctgccgg gatcaacgcc atcgagaacg agaggtgctc ttcctgggca atctcatgaa
     6241 ttatttctcg gcaccactcc aggtgtggag cggcaccaga accgccggcc gttccgatca
     6301 ccaccggtat attattttgc acgcctgcgg tgatcatata gcgcagatcg cgcttgaccc
     6361 ctgcgcgatc ggtaaacggt ttgcctgccc cgaggtagtg ggggcctgga tcggaagagc
     6421 ctgcatcaac ggcgatcaga tcgggtgatt ctgccatcgc tttgcggaag ctctcttccg
     6481 ggaatccata cccgaggatt gctgttggcg ataagacttt aaaagaacga gccatttgtt
     6541 aatccttagc ctgcaacagc gcgatagtac gattcatttc gttgaagacg atatcgaggc
     6601 cttcgtcaaa gcccatccct ggctttacca gcatacgcat agggcgagcg gcaagggcga
     6661 cgtggacaca ggtgcgggca ctgacatcag tttcattgca ggtgccgccc tggtacgctt
     6721 ccatgctgtg gctgttgcag taaagaaccg catcgacgat gttgtgaata ctgcccagat
     6781 ccggggtttt gatttgcacc atgtgacaac tggcggcatc agtgaaatca acaatatcct
     6841 ggtaggtgtt acaccattca tcggccacaa ttttcacgcc ggaaccgagg cgcgtcagct
     6901 ctttggtaat cgcggtcagc aggcgaattt gatcgggctt gttaccggca tcgaccggcc
     6961 cttcaatgta gagcggcagg ccttgcgctt ctttttccag gctggcgatg tattgcgcac
     7021 agcgaagcgg atccatatcg aagatcagac cgatagtgcc gtatacatcg atgtgcaggg
     7081 tagggtggta gcgtgcgctg gtgcgcttgc ttagaatgcg atccgacaac cagcggacat
     7141 attcgcgcag tttttcacct ttaaagccca gcttctcttc gacgttatta atcagcgcat
     7201 ggggcagcac gtcgatgccc ttgaggatca tcttatcgac ggcgatatat cgatcgtcgc
     7261 cgctctgacc aaataatgga atggattccg ccacgcgtgg caactgccat tcatcacaga
     7321 ccacttcagt tttcagacgg ccggttgcca gcgcggtagc atcaagcagc gcctgtgata
     7381 atccgtagcg cacggcggta tgcagcaagt tgccgtcaat acgcaatttg tcgaagaaac
     7441 gggcattcgg caggaaagca tccacatcgc ggcctaccag taatggcttg atatggtcgt
     7501 tgaggaacgg aataaagtgc tctgcgagga acagtggatc gcgaccacct gccccagaat
     7561 actgtacggc agcacagtca cctacagcga ccgcgccgtt ttccagaatc aactgtaccg
     7621 atacgcactc cccggcctga cgtacggtgt taaacccctg tgttactggc tccccggtat
     7681 aaaaaaagcc gtcgtgaccc gctccgtttt ttatcgcctg ctggtcatcg aaatagaatg
     7741 aggaatagcc agcggtgaac agggcctgtt ttattttcat tatggtcttc ctattaattt
     7801 actgtgggat acagcattga tgtcatctac aaccatctgg aatgtcggtt ggcgtccttc
     7861 acagcgtgcg cgttccgcaa cgtaatcgtg gtggaggtcg agaatatctt ttggcaatgg
     7921 aacggctccg gcttccagta cgcgtatcgc gccggcgttg tcacggaccg gaagaatttt
     7981 gcccgcgttg caggcggctg gcgcaaacgg cacatccagt acgcccgctt caaatgccag
     8041 caccgttccg cgcgcaatat caccattacc cagttcgaac actttattca gtactgcgcg
     8101 aacctcgctc ttaatcagtt caatttccag ttcaaccgca gggcacggcg ggaatttctg
     8161 ctcattgacc atgttgagca tctggcggga ggctttcagc ccctgaatgt tggcagcggc
     8221 agtcgggatg ccccaggctt cgtgcggact tttggtgatg actttggtgg caccagacat
     8281 ccccgcgacg gctgcgcccc aggagataat agcgaacgct ttcgattcgt cttccggaaa
     8341 gccgcccatc cactggtgaa acacggtact caattcatag tcggtatagc catagctctg
     8401 aaaatattcg tgtgcaagtt cacgcagaga ctggatcgcc gcaacatcct gcgtcaggct
     8461 gccgacctgg ccataaccaa cggtgataga tttcacaccc tgttccagtg ccagcaagcc
     8521 ttcgattatc gcgatagagt gcgagataaa cggcgggatc agcgtacccg ttagtgggcc
     8581 aaacggttcg cggttgatgc ggatcccgtg ttcttcatac atccccatta aacggtcgca
     8641 gtactgccag tcgcggatcg atttttccag cgttacgcgt ttggcatacg gaatgttgta
     8701 ggaaatgcca ccgccttcat aactggtaaa gccactggcc atcgagattt cagccagcag
     8761 acgcgcatct ggcgtgccgt ggcggatctg tagcggtttt tgcagggttt cggtgaggcg
     8821 acggcaagct gcgacaccat ggttaactac cggtaaaccg ttcagtttag aggttccggc
     8881 ttcgatggat tttttaatcc ctacagccgc ttcttcgtac cgattcagac gggtatatgc
     8941 atcgatggtg cttggcagca gatcgcactc ttcttgtagt gttttcagca gttcgatgtg
     9001 ttcgtccatc agcgccacac ctgcgcgcgg ttggctaagg gttttacctt ccttgtctgc
     9061 tttcagcagc gcaagagaga agcgtttatg ctcagggatg gtttgttggt atttaacgcc
     9121 atcttcaaag ttttcaacat ccttgccggt ttcccaggtt ttcagtacct gttgccgctc
     9181 ggtcatgaat tcgtcatggg ttaatttctt atttcgaagt tccattgatt gcagccttaa
     9241 ttgttatagg gttaagcatt gaatactggt gcgcgcggcg gccagcggat cgactctggc
     9301 tacgtttgcc agcagtggta atagcccccg ggaatcgcgg taatattcaa attcggtcgg
     9361 taagagaatg cgtttaccgt cgtcgtttaa ctcgcggtat ttcaaccagt gatgcatatc
     9421 gaactgactg gcacgcgaga gccatccacc ggaacccacg actttacgca cggttgtcag
     9481 gtcacgtccc atctgtaaat cgacattgcc gacgcaggtg cagacctctt ttttggtgcc
     9541 cgcatgtctt tcggtggcat aacccacaca cagtccggcc aggacggtat cgaaatattt
     9601 ctcttctgca tttgcgggta agtagtcggg atgagcgact agatgacgga ggtaatgata
     9661 aaaagcgtcc agctgtgcgg gttgctgagc aaaattcaca ttgactaact ctttcgcact
     9721 ttcaccaacc accactgccg agacgcgcat tcccagatcg ccttccaccg tccgttttac
     9781 gaagggttcc ggtacgccgt gtaaaacggt gtcgggcgag agcgtgttgg cacaggcgga
     9841 ataaacatcg gtggttgcgc cgcccatgtc gatcagcatg aactctttcc agctgtgatc
     9901 gacgttactg attgccttga ccagttcaaa gaccgtccat ggtgtcggca ttggttcttc
     9961 gcctgtttta tcaacaatga catccagccc tttcccttta acgatgcgtt taaggaatat
    10021 gtcgcaaata gcctggcgtg cggctaaagg gttcggatgg tcaagatcgg gcagaacgtt
    10081 gtcgacgatg gtcaggtctt tatgcccgag gatctcctgc acctcgtcct gaatatcccg
    10141 gtttccggca taaataatgg cgcagtcgag tttggattct gccagcacac gggcgttatt
    10201 cagtccgtag ctttcttctc cgccgtcggt gccgccggtg aacaagagaa tatcgggctg
    10261 cgtttcttcc agtgcctgga tatcacggcg gttcagcttg taggcgtaat attgcgcaat
    10321 tttagcaccc gcagagtgag cggttacttt cgcggtttcc agggtgatag aaggcaccaa
    10381 tcccattgcg gcaaccgcca gcccaccttt ggcagaggag gaatacttca atgcaacctc
    10441 accgctgttt aacaggggga gggcattatc tacgtttaac acctggttca ggctggagaa
    10501 aaaccccttc gccagatgat gggtggtggt tggggtcagt acgtggttaa ccaacgttaa
    10561 cgcgtccccc tcccgggtaa agagggctgc tttggtccac gtcgatccaa tatcgacaga
    10621 gacgatttgc atcagatagc ctcttcaaga atacgagtat caacgtcatg acgttggttg
    10681 atatcgtggg ccatcagctg acaaacctct tctaagtcat gacttggggc aaatacgcga
    10741 tcaaatccca tctctttgaa cttgttttcc acgtcagcga agtcatgttt tcctaccacc
    10801 aggttaccgc ccacatagag caggatgtcc cccagtccac gctcgatgca gcgttcgcgc
    10861 atgcccagac agtcgatatc gccatgccca tagatagagg agacgacaat cgcgtcagcg
    10921 ccagtttcaa tggcagcatc aatatattcg tcctggctga ccattacgcc cagattaatg
    10981 actctgaagt cgtggttgct aaaaacgcgg tccagaactt tattgcctac tgcatggcag
    11041 tccgcgccaa taacgccaat cacaagtgtt gctttcttca tgggtaatcc tttgtaagac
    11101 agagagtaaa ataagttaca cacgtcgtat ttatggccgt tcataacggc
//
LOCUS       DAZKFU020000148        11144 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZKFU020000148 DAZKFU020000000
VERSION     DAZKFU020000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.149"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..264
                     /locus_tag="ACHOQQ_RS16045"
                     /old_locus_tag="pgaptmp_003209"
     CDS             82..264
                     /locus_tag="ACHOQQ_RS16045"
                     /old_locus_tag="pgaptmp_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471422.1"
                     /translation="MHQKTAEHEQTRVLLTIKNGKVILIRHVHDDEFVGNLSTFLFIA
                     EKAGYDVIAPADEDEE"
     gene            270..842
                     /locus_tag="ACHOQQ_RS16050"
                     /old_locus_tag="pgaptmp_003210"
     CDS             270..842
                     /locus_tag="ACHOQQ_RS16050"
                     /old_locus_tag="pgaptmp_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471423.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPSPIRFALQQYIEWNLIQTYGEAEGMGRALFLYQKMLTTSPDKGF
                     ILSDIGREGLELLLDEMINDLNTHGMPEGQ"
     gene            1027..1215
                     /locus_tag="ACHOQQ_RS16055"
                     /old_locus_tag="pgaptmp_003211"
     CDS             1027..1215
                     /locus_tag="ACHOQQ_RS16055"
                     /old_locus_tag="pgaptmp_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471424.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTRAVISGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            1212..2039
                     /locus_tag="ACHOQQ_RS16060"
                     /old_locus_tag="pgaptmp_003212"
     CDS             1212..2039
                     /locus_tag="ACHOQQ_RS16060"
                     /old_locus_tag="pgaptmp_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4471425.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDSALKDIQAEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSDANTPFIQVK"
     gene            2080..2451
                     /locus_tag="ACHOQQ_RS16065"
                     /old_locus_tag="pgaptmp_003213"
     CDS             2080..2451
                     /locus_tag="ACHOQQ_RS16065"
                     /old_locus_tag="pgaptmp_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HGU4471426.1"
                     /translation="MNTHNAQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVISCAIESLYATLPYEEVEDERTGGTDIMATINIGELICQDEDLSGESW
                     LAGMLVAAEIISIEPATNIRL"
     gene            2483..2725
                     /locus_tag="ACHOQQ_RS16070"
                     /old_locus_tag="pgaptmp_003214"
     CDS             2483..2725
                     /locus_tag="ACHOQQ_RS16070"
                     /old_locus_tag="pgaptmp_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4471427.1"
                     /translation="MFDLINQGQLYTDSAGYPVKIIHCINNIVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            2729..2863
                     /locus_tag="ACHOQQ_RS16075"
                     /old_locus_tag="pgaptmp_003215"
     CDS             2729..2863
                     /locus_tag="ACHOQQ_RS16075"
                     /old_locus_tag="pgaptmp_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471428.1"
                     /translation="MNKAFELWVRQCYGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            2882..3136
                     /gene="xisR"
                     /locus_tag="ACHOQQ_RS16080"
                     /old_locus_tag="pgaptmp_003216"
     CDS             2882..3136
                     /gene="xisR"
                     /locus_tag="ACHOQQ_RS16080"
                     /old_locus_tag="pgaptmp_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4471429.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGVKNASSG"
     gene            3170..4456
                     /locus_tag="ACHOQQ_RS16085"
                     /old_locus_tag="pgaptmp_003217"
     CDS             3170..4456
                     /locus_tag="ACHOQQ_RS16085"
                     /old_locus_tag="pgaptmp_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4471430.1"
                     /translation="MSNTSYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGNFDYAAQFPESPNLKHFGLGKKEITIKALSEKWLDLKKIEIGGN
                     AFNRYQSVVRNMLPRIGESRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPVKGRSVV
                     TVNYYMTTIAGMFQFAADNGYTESNPFNGLTPLKRSRTEPDPLTHDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAIETLKSQAEMTRFGTQYQIDVKLREFGRTVSHECTFVFNPQLVKKCQQAGY
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALAAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKELAESVPYMPHRARR"
     gene            4458..5237
                     /gene="yecE"
                     /locus_tag="ACHOQQ_RS16090"
                     /old_locus_tag="pgaptmp_003218"
     CDS             4458..5237
                     /gene="yecE"
                     /locus_tag="ACHOQQ_RS16090"
                     /old_locus_tag="pgaptmp_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HGU4471431.1"
                     /translation="MISFSIIELTHGSRHVNCVEGNTTLYALPKPEVVLRWREQTTDD
                     FRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALW
                     HFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAARPHSE
                     AIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYL
                     FLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5290..5685
                     /gene="yecN"
                     /locus_tag="ACHOQQ_RS16095"
                     /old_locus_tag="pgaptmp_003219"
     CDS             5290..5685
                     /gene="yecN"
                     /locus_tag="ACHOQQ_RS16095"
                     /old_locus_tag="pgaptmp_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGU4471432.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            5726..6469
                     /gene="cmoA"
                     /locus_tag="ACHOQQ_RS16100"
                     /old_locus_tag="pgaptmp_003220"
     CDS             5726..6469
                     /gene="cmoA"
                     /locus_tag="ACHOQQ_RS16100"
                     /old_locus_tag="pgaptmp_003220"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGU4471433.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            6466..7437
                     /gene="cmoB"
                     /locus_tag="ACHOQQ_RS16105"
                     /old_locus_tag="pgaptmp_003221"
     CDS             6466..7437
                     /gene="cmoB"
                     /locus_tag="ACHOQQ_RS16105"
                     /old_locus_tag="pgaptmp_003221"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGU4471434.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(7602..10030)
                     /gene="torZ"
                     /locus_tag="ACHOQQ_RS16110"
                     /old_locus_tag="pgaptmp_003222"
                     /pseudo
     CDS             complement(7602..10030)
                     /gene="torZ"
                     /locus_tag="ACHOQQ_RS16110"
                     /old_locus_tag="pgaptmp_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(10055..>11144)
                     /gene="torY"
                     /locus_tag="ACHOQQ_RS16115"
                     /old_locus_tag="pgaptmp_003223"
     CDS             complement(10055..>11144)
                     /gene="torY"
                     /locus_tag="ACHOQQ_RS16115"
                     /old_locus_tag="pgaptmp_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGU4471435.1"
                     /translation="KRIGLLFLLIAVVVGGGGFLLAQKALHKTSDTAFCLSCHSMSKP
                     FEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKF
                     EAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGETCIDC
                     HKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTDLTVV
                     DASGKQPIVLLQGYQMQGSENTLYLAVGQRLALATLSEEGIKALTVNGEWQADEYGNQ
                     WRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIAKGMG
                     ARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2893 a         2736 c         2660 g         2855 t
ORIGIN      
        1 agcctgcctt attgcacaac acgggaaaaa ttacatgcat attgcctgct tgtttctgcg
       61 taaagcatac gggagataat aatgcatcag aaaacagcag aacacgaaca aaccagagta
      121 ttgctgacca tcaaaaacgg gaaagtaata ttaattcgtc atgttcatga cgatgaattt
      181 gtaggaaatc tttcaacatt cctgtttatt gcagaaaagg caggatatga cgttattgca
      241 ccagcagatg aagatgagga ataaatttca tgcaatacgc tgaattccag gctgaagcaa
      301 cagccaatgg tatacaaaca ggcagtatga ctattgatta tcacgacgcc atacgccgtc
      361 tggatgctgg agaattcgat actcctaatg tgcgaggttt acgtatcctt cagtgtctcg
      421 cgcaagctga cgaagcagga ttactgggaa aacttccggt tgagatgaag gttgcccagt
      481 ggcgatggtt gtatgtgacg acattcatca acgaagaaga agacaagaac ggcacaattg
      541 atgtcccgaa tgaacacgga acaacagatc gcgccgtaat atataacggg aagcatggag
      601 ttatgactat ataccctagt cccattcggt ttgccttaca gcagtatatt gaatggaatt
      661 taattcaaac atatggcgaa gctgagggaa tgggaagagc gctgtttctt tatcagaaaa
      721 tgctcaccac ttcccctgat aaaggtttca tcctttcaga tataggtcga gaagggcttg
      781 aactccttct ggatgaaatg attaacgacc tgaatactca tggtatgcca gaaggccagt
      841 gacacattaa atattaagaa gaatataact cttccgtttt ttactaaccg tttatatgaa
      901 aagcgaccgt gaattaagca gagtaaaact gcttttaatc cttgccacag tactgacact
      961 aacagaaatc attattctct ttattgcgct gtctgtcagt taaaaatatc gggataccac
     1021 ataccaatga gactgtattt cacaatagta attttactgg caattatcgc atgcatttac
     1081 ggattactcg ttccgttcct tatatccatg aaggatacga gagcagttat ttctggcttt
     1141 gcactggcgt ttctgacccc gccctgcatt tatgccattt acaagggtct ttctttcact
     1201 aaggataaaa aatgaaaaaa attatttttg ctttagccat tgttctgccg accattggtc
     1261 ttgtcggttg cgatcgcgtt gagcccggta atgttggcat caaagtaaat aaactgggcg
     1321 acgataaagg cgtcggtgag gtggtcggtg ttggtcgcta ctggactggc tggaatactg
     1381 aagtttacat cttccccacc ttcaagcaaa tgaagaccta cgatgaaccg ttcagctttc
     1441 agatgagtga cggcacaacc atcggctatc acatcggtgt ggcctacaag gttgatccat
     1501 ccaaagttac tacagtgttt caaacctacc gcaaaggcgt ggatgacatt accgacactg
     1561 acctgcgcca gaagatagcc gacgcactca accgactggc cagcaaaatg accaccgata
     1621 aatttatcga cggtggcaag tctgaactgc tggattcagc acttaaagat attcaggcag
     1681 aaatgacacc catcggcatt caggtaatga gcctctctta tgtcggtaaa ccggaatacc
     1741 cgccaaccgt gatcgacagc attaatgcca aagtcacagc gaaccagaaa accctgcaac
     1801 gcgaacagga agtaaaacag cgcgaagcgg aagccaacat gttgcgcgcg gaagcagccg
     1861 gacaggctga cgccattcgc acaaaagccc aggccgaagc cgatgctatt cgtttacgcg
     1921 gtgaagctct gcgccagaac cccggtgtta tggagctgga agccatcaat aaatggaacg
     1981 gcacactgcc gcaatacatg accagcgatg ccaatacacc atttatccag gttaaataac
     2041 ttatatgccc ggcaggccgc cgggctaagg gaaaagcaga tgaacaccca taatgcccaa
     2101 ccgcaaataa tgaactatga cccgaatctg acgtcatgcg gacgcatggc aaaacaaacc
     2161 gttcgattaa ctttcgggtt atgggaatac cgcgaaacat tcgaagttac tgtcggcggc
     2221 aatctgaccg gactggatgt tatcagttgc gctattgaaa gcctgtacgc aacactgcct
     2281 tatgaagaag tcgaggatga gcgcacaggg ggaacagata tcatggccac cattaatatt
     2341 ggcgaactga tatgtcagga tgaagacctg tccggagaga gctggcttgc tggaatgctt
     2401 gtagctgcag aaattatcag tattgaaccc gctacaaata tacggctctg aagttctcgc
     2461 tattcagaga acaggagaaa aaatgttcga tttgattaat caaggacaac tgtataccga
     2521 cagcgccggt tacccggtaa aaattattca ctgcataaat aacatcgtgt tgtacagaag
     2581 aatggatgga cgaacacagt cggtaaaaat aaacgatttt aatgaactgt ttgaacgaat
     2641 cgatcaccag gaataccgac aaattctggc agaaacagaa caagaagctc atctgaaaaa
     2701 attacgtgcc atgaaaagga agtaaagaat gaataaagcg tttgagctat gggtacgcca
     2761 gtgttacggc aatcgctatg acctgacgcg agatgttgac ggtttctact gccgtgaagt
     2821 tgtgaaacga atgtttgacg tgtggtgcca ctgccgtgga tgaaaatttt atgaggttgg
     2881 catgcagaca atcatctatc agataacccc cagcaaatgg tgtacggaga gagtcctcat
     2941 tgcatcaaca gggctaaagc ctggcaccat tgagcgggca agaagaaagt catggatgca
     3001 gggaaaagaa taccgccatt acgctgtaga aggtgatcca gggcattaca gtgaatgcct
     3061 gtacaacatc gaagaaatta tgcgatggat cgaaaaccag aaacaaccag gtgtcaaaaa
     3121 tgcaagttcc ggttaacctg ttaatgctcc tggacgtctg ggaggttcaa tgagtaacac
     3181 atcatacccg acaggcgttg aaaaccatgg cggatcactc cgcatatggt ttcactataa
     3241 cggcaaacgt gtcagagaaa acctcggtgt tcctgacaca gccaaaaacc ggaagattgc
     3301 aggcgaactc aggacttcag tttgttttgc aatcagaatg gggaatttcg actacgctgc
     3361 gcagtttcct gagtccccaa atctgaaaca ctttggtctg ggaaaaaagg agataaccat
     3421 taaggcgctt tcagaaaaat ggctggatct gaaaaaaatc gaaataggag gtaacgcatt
     3481 caatcggtat caatctgtgg tgagaaacat gttgcctcgt ataggggaaa gccgactggc
     3541 ttcatctgta acaaaagaag atttactctt catcaggaaa gatttattga ccgggtatca
     3601 caatctctca aacggcaaaa caacacctgt taaggggaga tctgtagtta cagttaatta
     3661 ctacatgacg acaattgcag gtatgtttca atttgcagct gacaacggct acacggaatc
     3721 aaacccgttt aacggcctga caccattaaa aaggtccaga accgagccag atccgctcac
     3781 gcatgacgaa ttcattcgtt ttattgatgc ttgccaccat caacaaacga aaaacctgtg
     3841 gtctttagca gtatacacag gcatccgtca cggtgagcta atatctctcg cctgggagga
     3901 tattgattta aaagctaaaa caatgactat ccgtcgtaat tatacaaaac tcggggagtt
     3961 tactctacca aaaaccgaag cgggaacaga tcgtgttatt catcttgttc aaccagccat
     4021 tgaaaccctg aaaagtcagg ctgaaatgac aagatttggc actcaatacc agattgacgt
     4081 caaactgcgg gagttcggac gtaccgtaag ccatgaatgc acgtttgtct ttaatccgca
     4141 actggtgaaa aagtgccagc aagccggtta ccactacaaa gcggactcca tcagggattc
     4201 atgggcatca gccctgagac gggccggact acggcacagg aaagcctatc agtcaaggca
     4261 tacttatgcc tgctgggcac tggccgcagg agcgaatcca agctttatcg caaaccagat
     4321 ggggcacgca aacgctcaga tggtattcaa tgtttacgga gcatggatga aagataacaa
     4381 tatcgggcag atagaactgc tcaataagga gttagctgaa agtgtcccat acatgcccca
     4441 cagagcaaga cgctgaaata atttctttca gtatcattga gttaacccat ggttcccgcc
     4501 atgttaactg cgtggagggc aacaccacgc tttacgccct gccgaaaccc gaggttgtcc
     4561 tgcgctggcg tgagcagacc acagatgact tccgcttctg ttttaagttt ccggcgacca
     4621 tttcgcatca ggcagcatta cggcattgcg atgatttagt gactgaattt ttgacccgca
     4681 tgtcaccgtt ggctccgcgc attgggcaat actggctgca actgcctgcc acattcggcc
     4741 cacgggagct gcctgcgctt tggcattttc tcgattctct ccctggcgaa tttaattatg
     4801 gtgtggaagt ccgccatcca cagtttttcg ccaaagggga agaggaacaa acgcttaatc
     4861 gcggtttaca tcagcgcggc gttaatcggg tgattttaga cagccgcccg gttcatgcag
     4921 cacgtccaca cagtgaagct attcgcgacg ctcaacgaaa aaaacctaaa gttccggtac
     4981 atgctgtact gacggcgaca aatccactga tccgttttat cggtagtgat gatatgacgc
     5041 aaaaccggga attatttcag gtctggttac aaaaattagc gcagtggcat cagaccacta
     5101 cgccttatct ttttttacat acgccagata ttgcccaggc cccggaactg gtacataccc
     5161 tgtgggaaga cttacgtaaa acgcttccag agatcggagc agttccggct attccacagc
     5221 aatcttctct tttctgaatt tgccacctat catagacagg tgccatcggc cattttaaag
     5281 ggagtttgta tggtaagcgc gctgtatgcc gttttaagtg cgttgttatt aatgaagttc
     5341 tcttttgatg tcgttcgcct gcgaatgcag taccgcgttg cctatggtga cggtggtttt
     5401 agcgaactgc aaagcgctat tcgcattcat ggtaacgcgg tggaatatat tcccatcgcg
     5461 attgtgttga tgctgtttat ggaaatgaat ggcgcagaaa cctggatggt gcatatttgc
     5521 ggcatcgttt tgcttgctgg tcgtctgatg cattattacg gttttcatca ccgtctgttt
     5581 cgctggcgac gttccggcat gagcgccacc tggtgtgcgc tgttgctgat ggtgctggcg
     5641 aatctttggt atatgccctg ggagttggtt ttctccctgc gttagcgcac aatacgccac
     5701 tttctttttc ccggattttt acgttatgtc tcaccgcgac acgctatttt ctgcccctat
     5761 cgccagactg ggcgactgga cctttgatga acgggtagct gaagtcttcc cggatatgat
     5821 ccagcgttcc gttcccggct attccaatat tatttccatg attggtatgt tagccgagcg
     5881 cttcgttcaa cctggtacgc aggtttacga tctgggttgt tctctgggcg cggcgacgct
     5941 ctcggtgcgt cgcaacattc atcatgataa ttgcaaaatt attgccattg acaactcccc
     6001 ggcgatgatt gaacgctgcc gtcgtcatat tgacgcctat aaagccccta cgccagtaga
     6061 cgtcattgaa ggtgatattc gcgatatcgc cattgaaaac gcatcgatgg tggtgctgaa
     6121 ttttaccctg caattcctgg aaccttccga gcgccaggcg ttactggata aaatttatca
     6181 agggctgaac ccgggcggcg cgctggtgct ttcggaaaaa ttcagtttcg aagatgccaa
     6241 agttggtgaa ctgctgttca acatgcacca cgactttaaa cgtgccaacg gttacagcga
     6301 actggagatc agccagaagc gcagcatgct ggaaaacgtg atgctgaccg attccgtgga
     6361 aacccataaa gcacgcctgc ataaagccgg ttttgagcat agcgagctgt ggttccagtg
     6421 ctttaacttt ggttcactgg tggcattaaa agcagaggac gctgcatgat cgactttggt
     6481 aacttttatt ctctgattgc caaaaatcat ctttcacact ggctcgaaac gctgcccgcg
     6541 cagattgcta actggcagcg cgagcagcag cacgggttgt ttaagcagtg gtcaaatgcg
     6601 gtggagtttc tacctgaaat taaaccgtat cgtcttgatt tattgcatag cgttacggca
     6661 gaaagcgaag agccactgag cgcagggcaa attaaacgca ttgaaacgct gatgcgcaac
     6721 ctgatgccgt ggcgcaaagg gccgttctca ctgtatggcg tcaacatcga taccgaatgg
     6781 cgttccgact ggaaatggga tcgtgttctg ccccatcttt ctgatttaac cggacgcacc
     6841 attctggatg tcggctgcgg cagcggttat cacatgtggc gcatgattgg cgcaggcgcg
     6901 cacctcgcgg tggggatcga ccctacgcaa ctgtttctgt gccagtttga agcggtgcgt
     6961 aaactactgg gtaatgatca gcgcgcgcat ttgttaccgt taggtattga acaacttccg
     7021 gcactgaaag cctttgatac cgtcttttcg atgggcgtgc tttatcatcg tcgttcaccg
     7081 ctggagcatc tctggcagtt gaaagatcaa ctggtcaatg aaggtgaact ggtgcttgaa
     7141 acgctggtta ttgatggcga tgagaacacg gtgctggtgc cgggcgatcg ttacgcgcaa
     7201 atgcgtaatg tctatttcat cccttccgcg ctggcgctga aaaactggct gaagaagtgt
     7261 ggttttgtag atatccgcat tgcggatgtg agcgttacca ctacagaaga gcagcgacgc
     7321 actgaatgga tggttaccga gtctctgtcc gatttcctcg acccacatga tccgagtaag
     7381 acagttgaag gttatcctgc gcctaaacgc gcggtgctga ttgcgcgcaa gccgtaaagg
     7441 tctggtatta ctgccggatg cggcgtgaac gccttatcca gcctacaaaa ccttgctaat
     7501 tcaatatatt gcagggacta tgtaggcctg ataagcatag cgcatcaggc agctttatgt
     7561 ttgcataacc tcagcgcccg tttccgggcg cttttcacgt cttacgcctg aacagctggc
     7621 tgatcaaacg ccgttaactt cggtgcgtta cccgtatatt tttcgatata caccagcgca
     7681 gagttaccgg cacaggcatt tgccagctgc gagctgggga tatccgccgt taacacgttc
     7741 gcactgccgt ttttacacaa gccattttcc agatctggcc atgcaccttc atgcacgcat
     7801 accacgcctt ttttgatccc gtcagtcacc accgcacctg tcagaatctg accacgtttg
     7861 ttccagacac gcaccagatc atcattcgca ataccaaagc gagcagcatc ttcggtgtga
     7921 atagtaattg gttcacgatc ggcaaccgca tattttttac gcagttccgc atagttaagc
     7981 tgactgtgta aacggtgtgc cggatgtgcg gtcagaagct gcaactgctt ctcgtcggcg
     8041 gtacccttcc actcatcagg cgcaagccag gtcgggtgtg ccgggcaatc cttatagcca
     8101 aatttctcca gcgtttttga gtagatttca attttgccac ttggtgtacc cagcgcattt
     8161 ttcaccggat ctgcgcggaa atcgccatag cgaacgaact gctcattctt ctcgctgcgg
     8221 cgcatttcaa gcaatttatt ttgctgccag aaggcattaa acatcggcat ggtgacgcgt
     8281 tgcgcacgcg cacctttctg agcggcatca tagaaaaatt tcagccacgc catttcatct
     8341 ctaccttcgg tatagatctc ttttccgcct ggtttcagta attccgcaag gtcggcaaac
     8401 acgtcaaaat cgttgcgtgc ttcaaattgc ggagcgacaa cctgcttcat cggcacaata
     8461 tgttggttac tgtaatcgcc ggtcatcgtc agatcattgc ctcgaacgat gtggtgatcg
     8521 gtaatacgat atccgcgtgt ttagctgccg ccgtccagta gcactcagaa acgacgatca
     8581 tctccggttt ctgccaggct ttaatcagac gattagtatc ctggtgatgg gtaaagttac
     8641 cgccacccgc ccaccagatc attttgatat tcggatacgt ttgttcttta ccgttatgct
     8701 gatatttgcc gccaggattt tccagcgcat cgacgatacg ggcaacagga atcgccgtca
     8761 ttccgccatc atcagcggcc tcgctggcct gtccggcaat cgccgcagac atttccggca
     8821 gcacgccgcc aacacgcgtc gggttgccac cgttggagta atgataagag aaaccaaaac
     8881 cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag cgtcaccagc atccagtgtt
     8941 tctgttcacc atattgctgg cgctgaattc cccagcctgc catcagcatt gtgcgattcg
     9001 ccgccatcag ttcagcgagt ttgactatct gcgcttccgg tacgccagta atttccgccg
     9061 cccaggccgc gctcttcgga gtattatcgc tcttacccgt cagatactct tcaaactgcg
     9121 gatacccggt ggtgtatttt tcaaggaaca ctttatcgtg tttgccttgt gtcatcaggg
     9181 tatgcgcaat ccctaacatc agtgccacgt cggtgcccat attcggcgcg atccaggtgg
     9241 cattatcgcc aaagaactcg atggtttcgg agcggatagg atcaatggca atcactggtt
     9301 tgccagattt tttcagctga tggaagtatt ccagcccttg ctcatcggta ctgctccagg
     9361 caatttttaa ggtattcagc gggttcattc cccacagcac cacaacctgg ctgttttcca
     9421 gaatcagcgg ccaggaggtc tgctgttcat acacctctac agaaccgacc acatgcggca
     9481 tgatcacctg tgctgccccg gttgaataat cgccgctatg cccggaataa ccgcccgcca
     9541 ggttcatata acgttgcagt aaggtttgcg ctttatgcaa cacgccagaa gagcgccagc
     9601 cgtaagatcc ggcaaaaatg gccgatggtc cgttagcttt acgaatacgc tcatgttgct
     9661 catgaatcag ttttaatgct tgctcccagc tcacctgtac ataggtatct tcgcctcgac
     9721 ctttcgccgg ttgcagtgga ttatcgagat agctttttct caccatcgga tgctgaatac
     9781 gcgccgtggt gtgtacctga tccgccgccg tagactgtaa ggagttcggt atggttttcg
     9841 ccaacgcgcc tgtcgaagaa acaatcttgc cgtccttcac ttctacgttc atcgctcccc
     9901 aacgtcccgc ggtgaggatt ttaccgccct tctcttctgc ccatgcgggt agcggtgctg
     9961 ccgatgtcac caccagcgct ccagcggcaa taccgctgtg tttaataaat tcacgtcttg
    10021 ttaatgtcat aacttcctcc ctgatcaacg aggatcactg tttctcggta atatctttgg
    10081 cgttgtactg gaaataccgc gttaaaatgt ccagttcgtt ttcgctcatg ctggttcgtg
    10141 cccccattcc tttggcaatg gaaggccacg cattgacggt gtaatggtcg gcggcaatag
    10201 gggcatgaca accagcgcaa taggtatcgt caagtttttc agcgtattgc catagcggtt
    10261 tacggtccgc taatgcggga tcggtaagcg caccctgtaa agacgcctga cgccattgat
    10321 tgccgtattc gtcagcctgc cattccccgt ttaccgtgag cgccttgata ccttcttcac
    10381 ttaatgtggc tagcgccagc cgttgaccta ccgccaggta gagcgtgttt tcactgccct
    10441 gcatttgata accctgcaac agaacgatcg gctgtttgcc actggcatca acgacggtga
    10501 gatcggtacc aggattcacg gtagccagct cgcctatgcg agaagttttg aaaggataaa
    10561 tatgtgcgcc attagtcact gaagtagcgg cctgactttc cagctcatgc gccgcgttgt
    10621 catccatttt tatttctggc ggaaaatggg caatgccttt atgacaatcg atacaggttt
    10681 cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga ttcactttgc gaggcaatat
    10741 ccatggcatc aaaagaatgg caactacggc acgttgcaga gtcagttgct tttaattctt
    10801 tccatactgt ttcggccatt tcctggcgat gagcttcgaa cttatcgtca ctgtctattt
    10861 tgccgctaac aaattcatga taaatatctt tagatgcttt taatttagcg aataaataat
    10921 ccatccctga ctttggaata tggcaatcgg cacattccgc acgtatccct ttctggttcg
    10981 aaaagtggac agttccctga tattcctcaa aaggtttact catcgagtgg caggaaaggc
    11041 aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc cagcaagaac ccgccgccac
    11101 caaccacaac cgctatcagc aaaaacaata acccaatgcg tttt
//
LOCUS       DAZKFU020000149        11118 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZKFU020000149 DAZKFU020000000
VERSION     DAZKFU020000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.150"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(164..1993)
                     /gene="glmS"
                     /locus_tag="ACHOQQ_RS16120"
                     /old_locus_tag="pgaptmp_003224"
     CDS             complement(164..1993)
                     /gene="glmS"
                     /locus_tag="ACHOQQ_RS16120"
                     /old_locus_tag="pgaptmp_003224"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGU4471436.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2155..3525)
                     /gene="glmU"
                     /locus_tag="ACHOQQ_RS16125"
                     /old_locus_tag="pgaptmp_003225"
     CDS             complement(2155..3525)
                     /gene="glmU"
                     /locus_tag="ACHOQQ_RS16125"
                     /old_locus_tag="pgaptmp_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGU4471437.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELQEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3878..4297)
                     /gene="atpC"
                     /locus_tag="ACHOQQ_RS16130"
                     /old_locus_tag="pgaptmp_003226"
     CDS             complement(3878..4297)
                     /gene="atpC"
                     /locus_tag="ACHOQQ_RS16130"
                     /old_locus_tag="pgaptmp_003226"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGU4471438.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4318..5700)
                     /gene="atpD"
                     /locus_tag="ACHOQQ_RS16135"
                     /old_locus_tag="pgaptmp_003227"
     CDS             complement(4318..5700)
                     /gene="atpD"
                     /locus_tag="ACHOQQ_RS16135"
                     /old_locus_tag="pgaptmp_003227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGU4471439.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRCLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5727..6590)
                     /gene="atpG"
                     /locus_tag="ACHOQQ_RS16140"
                     /old_locus_tag="pgaptmp_003228"
     CDS             complement(5727..6590)
                     /gene="atpG"
                     /locus_tag="ACHOQQ_RS16140"
                     /old_locus_tag="pgaptmp_003228"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGU4471440.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6641..8182)
                     /gene="atpA"
                     /locus_tag="ACHOQQ_RS16145"
                     /old_locus_tag="pgaptmp_003229"
     CDS             complement(6641..8182)
                     /gene="atpA"
                     /locus_tag="ACHOQQ_RS16145"
                     /old_locus_tag="pgaptmp_003229"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGU4471441.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8195..8728)
                     /gene="atpH"
                     /locus_tag="ACHOQQ_RS16150"
                     /old_locus_tag="pgaptmp_003230"
     CDS             complement(8195..8728)
                     /gene="atpH"
                     /locus_tag="ACHOQQ_RS16150"
                     /old_locus_tag="pgaptmp_003230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGU4471442.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8743..9213)
                     /gene="atpF"
                     /locus_tag="ACHOQQ_RS16155"
                     /old_locus_tag="pgaptmp_003231"
     CDS             complement(8743..9213)
                     /gene="atpF"
                     /locus_tag="ACHOQQ_RS16155"
                     /old_locus_tag="pgaptmp_003231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGU4471443.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9275..9514)
                     /gene="atpE"
                     /locus_tag="ACHOQQ_RS16160"
                     /old_locus_tag="pgaptmp_003232"
     CDS             complement(9275..9514)
                     /gene="atpE"
                     /locus_tag="ACHOQQ_RS16160"
                     /old_locus_tag="pgaptmp_003232"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGU4471444.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9561..10376)
                     /gene="atpB"
                     /locus_tag="ACHOQQ_RS16165"
                     /old_locus_tag="pgaptmp_003233"
     CDS             complement(9561..10376)
                     /gene="atpB"
                     /locus_tag="ACHOQQ_RS16165"
                     /old_locus_tag="pgaptmp_003233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGU4471445.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10385..10765)
                     /gene="atpI"
                     /locus_tag="ACHOQQ_RS16170"
                     /old_locus_tag="pgaptmp_003234"
     CDS             complement(10385..10765)
                     /gene="atpI"
                     /locus_tag="ACHOQQ_RS16170"
                     /old_locus_tag="pgaptmp_003234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGU4471446.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2663 a         3011 c         2760 g         2684 t
ORIGIN      
        1 gtcaatgatt cgaggtattt aattactact acggttaaaa caagtgttca acggtgcccc
       61 aaataatagc tgaagttaat tatatggaat gaggttagat tattaatgct ggtaagtaaa
      121 agggccgcca aaaggcagcc cttcgttcag caaatgaata atattactca accgttaccg
      181 attttgccag gttacgcggc tggtcaacgt cggtgccttt gatcagcgcg acgtgataag
      241 ccagtaactg cagcggaacg gtgtagaaga tcggtgcaat cacctcttcc acatgcggca
      301 tctcgatgat gtgcatgtta tcgctactta caaaacccgc atcctgatcg gcgaagacat
      361 acaattggcc gccacgcgca cgaacttctt caatgttgga tttcagtttt tccagcaatt
      421 cgttgttcgg cgcaacaacg ataaccggca tatccgcgtc aatcagcgcc agcggaccgt
      481 gtttcagttc acctgcagcg taggcttcag cgtgaatgta agagatctct ttcagcttca
      541 atgcgccttc cagcgcgatt gggtactgat cgccacggcc caggaacagc gcgtgatgtt
      601 tgtcagagaa atcttctgcc agagcttcaa tgcgtttgtc ctgagacagc atctgctcaa
      661 tacggctcgg cagcgcctgc agaccatgca caatgtcatg ttcaatggag gcatccagac
      721 ctttcaggcg agacagcttc gccaccagca tcaacagcac agttaactgg gtggtgaatg
      781 ctttagtgga tgccacgccg atttctgtac ccgcgttggt cattagcgcc agatcggatt
      841 cgcgcaccag agaagaaccc ggaacgttac agattgccag tgaaccaagg taacccagct
      901 ctttcgacag acgcaggccc gccagggtat ctgcggtttc gccagactgt gacaaggtga
      961 tcatcaggct gttacgacgc acggcagatt tgcgatagcg gaattcagag gcgatttcga
     1021 cgtcgcacgg aatacctgcc agcgattcaa accagtaacg ggaaaccata ccggagttat
     1081 aagaagtacc acaggcgagg atctgaatat gctcaacctt cgacagcagt tcgtcggcgt
     1141 tcggtcccag ctcgcttaaa tcaacctgac cgtggctgat gcgcccggta agggtgtttt
     1201 tgatcgcgtt cggctgttcg tagatctctt tctgcatgta gtgacggtaa atacctttat
     1261 cgcccgcgtc atattgcaga ttggattcga tatcctgacg ttttacttcc gcgccagttt
     1321 tatcgaagat gtttaccgaa cggcgagtaa tttccgcaat atcgccctct tcaaggaaga
     1381 taaagcgacg ggtcaccggc agcagcgcca gctggtcaga agcgataaag ttttcgccca
     1441 tacccagacc aatcaccagc ggactaccag aacgtgctgc cagcagggta tccgggtgac
     1501 gggagtccat gatcactgta ccgtacgcac cacgcagttg cgggatagca cgcagaacag
     1561 cctcacgcag agtaccgccc tgtttcagct cccagttcac cagatgggca atcacttcgg
     1621 tgtcggtttc agaaacgaag gtatagccac gcgcttttaa ctcttcacgc agcggttcat
     1681 ggttttcgat gatgccgtta tgcaccacca caatgtgttc agaaacatgc ggatgcgcgt
     1741 taacttctga aggttcacca tgggtcgccc agcgagtgtg agcaataccg gtgccgccat
     1801 gcagaggatg ttcttccgct gcctgagcca gcatctggac tttaccgagg cgacgcaggc
     1861 gggtcatatg accttccgca tcaacaacgg ccagaccggc agagtcatat ccgcggtatt
     1921 ccagacgacg taaaccttca agaaggattt ctgctacatc acgttgcgcg atcgcgccaa
     1981 caattccaca catagttttt gattccgatt tatatcgttg tcggtcaacc tgtatgcccg
     2041 ttttttcggg cgccccgagc cttgtagaga gtggggttat ttttatagtt actgcttgtg
     2101 ggagggagat tatgttatct cctcatccca tgtgaccgga ttacccggcc agaatcactt
     2161 tttctttacc ggacgacgcc agccttcttt ctgagtctgc ggcacacggc tgatagctaa
     2221 tgcattttcg ccgacattac gcgtcacagt tgtacccgca gcaatggtcg cgcctttgcc
     2281 tactgttacc ggggccacca gctgagtgtc ggaaccgaca aacacatcgt cgccgataat
     2341 ggtcttaaat ttattcgcac catcgtagtt gcaggtaatg gttcccgcgc cgatgttaac
     2401 gttatcgcca atttccgcat cgcccaggta agtcagatga ccagctttcg agcctttacc
     2461 cagacgcgct tttttcatct caacaaagtt accgacgtgt gcaccttcct gcaactcagc
     2521 accaggacgc aaacgggcaa acgggccaat ggtacaggcc gccgccagat tcgcgtcttc
     2581 cacgacggta tatgggctaa tttcgcaatc atcgccaatc acgctgtttt taatcacgca
     2641 accggtgccg attttcacgc gatgaccgag agtcacgttg ccctcgatga taacgttagt
     2701 atcaatttca acatcgcgcc cgtgagtaag cgtaccacgc agatcaaaac gcgccggatc
     2761 gcgcagcata acgcctgcta acagcagttt ttcagcctgt tcggactgat aaacacgctc
     2821 cagacgggag agttgcaggc ggttattcac gccttctact tcgcttaaac gttgcggatg
     2881 aacggcgacg atttcacgcc cttcctgata cgccagtgca ataatgtcgg tgatgtagta
     2941 ttcgccctga gcattattgt tggtcagctt cgccagccag cgtttcatat ctgcgccgtt
     3001 agcgatcaga atgccggtgt tgatctcctg aatctgacgc tgctcgtcgg tggcatcttt
     3061 gtgctcaaca atgccggtaa ctttgccgtt ttcacgggtg atacgtccat aaccggtcgg
     3121 atcatccagt ttcaccgtca gcagaccaat gccaccctgc ggtttagcat cacgcagacg
     3181 ctggagtgtt tcgacagaga tcagcggcac gtcgccgtag agcattaaaa tgtcttcatc
     3241 atcggcaaag aaaggtgcag cctgctgcat tgcatgacca gtacccagct gttctgcctg
     3301 aagcacccag ttcaggttgt cgtctttcag cgcctgtttt agcagatcgc cgccgtgacc
     3361 gtacaccagg tgaacgtgcg ctgcgcctaa ttcattcgca gcatcaatga catgctgaac
     3421 catcgctttc ccggcaaggg tatgcagcac tttcggaaga tcggaataca tgcgcgtgcc
     3481 tttacctgcg gcaaggatca ctacgctcat agcattattc aacatacgcg tcctgactgt
     3541 aatttgagaa cgaatttaaa ccgcttcacc ttgaaaaaac tacatttttt tcatcgtgaa
     3601 atggacagag gataaattgt tcaataacgg attatccccg ctgacgaaag cgccattttc
     3661 gaccatcgtc acgccttttg tctgcagaaa attaaggcag taccttcatt taagcgtcag
     3721 gtggatgttt ttgctcttat ttcgatcaat gaataaacgg gaaaacaggg ggttttatat
     3781 cagcaggatc tatgtgaacg ctattcagga cgggtcacac gcgcaaaaaa aaagccagcc
     3841 tgtttccaga ctggcttttg tgcttttcaa gccggtgtta catcgctttt ttggtcaact
     3901 cgataacgcg cagctgcgcg atcgctttgg ccagttccgc agacgcctga gcgtaatcta
     3961 cgtcgccgtg agagctgcta atgtgctctt cagccttacg tttcgcttcc atggctcgcg
     4021 cttcgtcgag atcctggccg cgaattgcgg tgtcggccag aacggtcacg ttgccaggct
     4081 gcacttcaag aatgccgcca gacagataga taaactcttc gtgaccgtgc tgtttcacga
     4141 tgcgaatcat accaggctta atggcggtga gcagcggtgc gtggccaggg tagatcccca
     4201 gttcaccttc gctacccgtt acctggattt tctcgaccag accagagaac atttgttgct
     4261 ctgcgctgac gacgtccagg tggtaagtca ttgccatatc accctccgat taaggcgtta
     4321 aagttttttg gctttttcca cagcttcttc gatggaaccg accatgtaga acgcctgctc
     4381 cggcaggtga tcgtattcgc cttccatgat gcctttaaag ccacggatgg tgtctttcag
     4441 ggagacgtat ttacccggag aaccggtgaa tacttctgcc acgaagaacg gctgggacag
     4501 gaagcgctgg atcttacgag cacgtgctac caccagtttg tcttcttcag acagttcatc
     4561 catacccagg atggcgatga tgtctttcag ttcctgataa cgttgcagga tggactgaac
     4621 gccacgcgcg gtgtcgtagt gttcctgacc aaccaccagc gggtccagct gacggctggt
     4681 ggagtccagc gggtcaacgg ccgggtagat acccagagac gcgatctgac ggctcagtac
     4741 cacggttgcg tcaaggtgcg caaaggtggt tgccggagac gggtcagtca agtcatccgc
     4801 aggtacgtat actgcctgta cggaggtgat agaaccggtt ttggtggagg tgatacgttc
     4861 ctgcagaacg cccatctctt ccgccagggt cggctgataa cctaccgctg aaggcatacg
     4921 gcccagcagt gcggatactt ccgtaccggc cagggtgtaa cgatagatgt tgtcaacgaa
     4981 cagcagaacg tcacgacctt cgtcacggaa tttctcagcc atggtcagac cggtcagagc
     5041 aacgcgcaga cggtttcccg gcggctcgtt catctggcca tacaccaggg atactttgtc
     5101 gataacgttg gagtcggtca tttcgtggta gaagtcgtta ccctcacgag tacgttcacc
     5161 tacgcccgca aacacagagt aaccggagtg ctcgatcgcg atgttacgaa tgagctccat
     5221 catgtttacg gttttaccta cacccgcacc accgaacaga ccaactttac cgcccttagc
     5281 gaacggacac atcaggtcga taactttgat accggtttcc agcagttcct gagagtttga
     5341 cagctcttcg taggaaggtg ctgcgcggtg aatcgcccaa cgctcttctt caccgatctc
     5401 gcctttcatg tcgaccggtt cacccagtac gttcatgata cggcccagag tcgctttacc
     5461 taccgggact tcgatcgggt gttcgaggtc ttttacatcc agacagcgac gcagaccgtc
     5521 ggaggaaccc attgcgatgg tacgcacgat accgccgccg agctgctgct gaacttccag
     5581 caccagacgc tcattaccat tttgcacctc aagagcatcg tacacgcgcg gtacggcatc
     5641 ctgagggaat tcgacgtcaa ctacggcgcc gattacctgg acaatctttc cagtagccat
     5701 cttaaatcct ctacgaaata acctgtttaa accgcggcgg cccccgagac gatctcggtg
     5761 agttcctgag taatgctggc ctgacgagct ttgttgtata ccaactgcag ctctttaatc
     5821 aggctgccgc cattgtcggt cgcggctttc atcgccacca tacgggcggc ctgctcgctg
     5881 gccaggtttt caaccacgcc ctgataaacc tgagattcga cataacgacg cagcagggta
     5941 tccagcaacg ccttcggatc gggttcgtac aggtaatccc aggatttatg tttcagatca
     6001 tcatcatctg atgccggtaa cggcagcagc tggctgatgg tcggaacctg agacatggtg
     6061 ttaataaatt tgttgctgac aatgtaaagc ttgtccagac ggccttcgtc gtaggcctgc
     6121 aacatcactt ttaccggacc gatcagttcg gacagggaag ggttatcccc catgccggtg
     6181 acctgggcaa caacattgcc gcccacggag ttgaagaacg acacgccttt cgagccgatc
     6241 attgcgaggt cgcattgaac gcctttgtcg gtccaggtct tcatttccgc cagcagtttt
     6301 ttgaacaggt taatgttcaa accaccgcac aaaccacggt cggtcgacac caccaggtag
     6361 cccacgcgtt taacgtcgcg gtcttccagg taagggtgct tgtattccag attaccgtgt
     6421 gcaaggtgac caatcacttt gcgcatggtt tctgcataag gacggctggc cgccatgcga
     6481 tcctgcgatt tacgcatttt ggaagcggcg accatctcca tcgctttagt gatcttttgc
     6541 gtgttctgga cgcttgcgat cttactacgt atctcttttg cgccggccat gagcttctcc
     6601 tcaatgcctt gcggcctgcc ctaaggcaag ccgccagacg ttaccaggat tgggttgctt
     6661 tgaaggaatc gaggatgcct ttcagcttgc cttcgatttc gtcgttgtag ccaccggtct
     6721 ggttgatctc ttgcatcaac ggagcgtgat cacggtcgac gtaagccagc agagcggctt
     6781 cgaagctgcc gattttcgac agttcaacat ccgccaggta accacgttct gctgcgaaca
     6841 gaaccagaga ctgttgcgct acggacatcg gcgcatactg tttctgtttc agcagttcgg
     6901 tcactttctg accgtggtca agttgcttac gtgttgcatc gtcaaggtcg gatgcaaact
     6961 gagagaacgc tgccagttca cgatactgtg ccagagcggt acggatacca ccggacagtt
     7021 ttttcatgat cttggtctgt gctgcaccac caacacggga tacggaaata cccgggttaa
     7081 ccgcaggacg aataccggcg ttgaacaggt tggtttccag gaagatctga ccatcggtaa
     7141 tggagattac gttggtcgga acgaacgcag aaacgtcacc cgcctgagtt tcgataatcg
     7201 gcagtgcggt cagagaaccg gttttccctt tcacttcacc tttggtgaag gcttcaacgt
     7261 attcggcgtt aacacgcgca gcacgctcca gcagacgaga gtggaggtag aatacgtcgc
     7321 ccgggaatgc ttcacgtcct ggcggacgac ggagcagcag ggagatctga cggtaagcaa
     7381 cagcctgttt agacaggtca tcgtaaatga tcagcgcatc ttcaccgcgg tcacggaagt
     7441 attcgcccat tgcgcaaccg gcatacggtg ccaggtattg cagtgcagcg gattcagacg
     7501 cggttgctac cacaacgatg gtgttagcca gtgcgccgtg ctcttccagt ttacgtacca
     7561 cgttagaaat ggtggacgct ttctggccga tagcgacata gatacatttg ataccggaat
     7621 cgcgctggtt gatgatggca tcgatagcca gtgcggtttt accggtctga cggtcaccga
     7681 tgatcaattc acgctgacca cgaccgattg ggatcatgga gtcaacggct ttataaccgg
     7741 tctgtaccgg ctgatctacg gactgacgtt cgataacgcc cggagcgatt gcttctacag
     7801 cagagaagcc gtcgtgatcc agcggacctt taccgtcgat tggtgcaccc agagtgttaa
     7861 ccacacggcc cagcaggcca cggccaaccg gaacttccag gatacggcca gtacacttaa
     7921 ctttcatgcc ttcggcaagg tcagcgtacg gacccataac aaccgcacct acagagtcgc
     7981 gctcgaggtt cagtgcgata gcgtaacggt tacccggcag ggagatcatt tcaccctgca
     8041 tacaatcggc caggccgtga atgcggataa caccgtcact tacagaaaca atagtacctt
     8101 cgttgtgagc ttcactcaca acattgaact gagcaatgcg ctgcttgatc agttcgctga
     8161 tttcggtgga attcagttgc atgctccagt ccccttaaga ctgcaagacg tctgcaaggc
     8221 gctcaagacg accgcgtacg ctgccatcaa tgaccatatc acccgctcgg atgataacgc
     8281 ctgccattac agacttatcg attttgcaat tcagcttaac tttgcgtgac agacgttttt
     8341 ccatcgcagc agaaattttc gcgagctgtt gttcactcag tgcggcagcg gaaatgacgt
     8401 ctacctcagc ggtagcctca ctcacggcac gaaggtgaat aaactgctcc agaacatccg
     8461 ggagcgcgtt aagacgacca ttttcagcca ttacccgaat aaggttctga ccgttttcgt
     8521 ccagttgctc accacaaact gcgataaacg actcggcaag cgtttctggc gcaagcgcgc
     8581 cagagagaag ctctgccatt tgttcgtttt tggttacctc ggcggcaaac gccagcatgt
     8641 cctgccagcg ttctacactt tggtgttcga cggcaaagtc aaaagctgct ttggcgtagg
     8701 ggcgagctac cgtaataaat tcagacatca gcccctccct ccttacagtt cagcgacaag
     8761 tttatccacg atgtcgctgt tagcagcttc atccacggaa cgttcgatga tcttctcggc
     8821 gccagcaaca gccaggatag caacttgctt acgcagctct tcacgggcac gtttacgctc
     8881 ggcttcaatt tccgcctgcg cctgggccac gattttagta cgttcctgtt ctgcctcagc
     8941 tttcgcttcg tccagaatct gcgagcggcg tttgttcgcc tgctcgatga ttacctgggc
     9001 ttccgctttc gcttttttca gctggtcggt cgcgctggcc tttgcaaggt caaggtcctt
     9061 atgtgctcgt tctgcggaag caaggccgtc agcaatttct ttttgacgtt tttcgatggc
     9121 tgccattaat ggcggccata cgtacttcat gcagaacaga acgaacagga caaacgcgat
     9181 ggcctggccg aggattgttg cgttaagatt cacagcacaa tgcctctatt tagttaacgt
     9241 tctgatattg ctctttaaat aaaagcaacg cttactacgc gacagcgaac atcacgtaca
     9301 gacccagacc tacagcgatc atcgggatag catccaccag acccataacg ataaagaact
     9361 gagtacgcag cagaggaatc agatcaggtt gacgcgctgc gccttccagg aatttacccc
     9421 cgaggatgcc gataccgatc gcagcaccga ttgccgccag acccatcatc acagcggcag
     9481 ccatgtacag cagatccata ttcaggtttt ccatgacagt ctccagtttg tttcagttaa
     9541 aacgtagtag tgttggtaaa ttaatgttct tcagacgcca tcgacagata gacgatcgtc
     9601 agaaccatga agatgaaggc ttgcagcgta atgatcagga tgtggaaaat ggcccacggc
     9661 acattcagga tccactgtga ccaccacggc aacagaccag caatcagaat gaaaatcagc
     9721 tcaccggcat acatgttacc gaacagtcgc aaaccgagtg aaactggttt ggacagcagg
     9781 cttacccctt caaggattaa gttgacagga atgaacgccc agtgattgaa cggctgcagc
     9841 gtcaactctt tcgtgaagcc gccgatgcct ttcattttga tgctgtagaa cagaatcagg
     9901 ataaatacgc ccagtgccat agacagcgtt acgttcacgt ccgcagacgg aaccacacgc
     9961 agtgcaggca gacccagtac atgttcagca atgtacggca gcaggtcgat aggcagtaaa
    10021 tccatcaggt tcatcaggaa tacccagacg aagatcgtca gggccagcgg agcaatcagc
    10081 ttgcttttgc catggtacat gtctttcacg ctaccattaa caaagccgat caccagctca
    10141 atcgcggtct gaaacttacc tggcacaccg ctggtcgcct ttttggctac gctacggaat
    10201 aaaaccagga acaacagacc cagcaccacc gagaagaaca tggagtcaat attgattgtc
    10261 cagaaggtgg ctggggggtt ttgtggatcc accagcgaga atgtacgcag gtccagctga
    10321 aggttattca ggtggtgtcc tatgtaatcc tgcggcgtca tattttctga agccatgatg
    10381 ccttttaccc tttgttgtta attacagccg gtgccagtat ctgaaccacc agcaccaaaa
    10441 cccacgtaac gatcagcggc aagaataccg cctttaaaac cgccaacgcc accaccagta
    10501 acaccaacat cgccagaact ttgaaagctt cgccaaatgc gaatgtccag gccacccggc
    10561 ctttcgctgg tgtatgcgcc tggtgacgcc aggcaaatat cataaacaaa acgttaggca
    10621 gaaagactgc caggcccccg cttattgcag agacgcccca gaaggggtct ttgaggctga
    10681 acagcaatcc acttgctatc accaccagta actgaacgag cagaagcttc cgagcaacgt
    10741 ttcgactcac gagcgacaca gacatcacgt ttttcactcc tgctcccttc gaggtatgcc
    10801 gcgtgtcgta taaaactttc tttaaggctt agagtcaagc atcaaaaagc ggtcaaatta
    10861 tacggtgcgc ccccgtgatt tcaaacaata agtagccaaa aggtgaataa atgtttaaat
    10921 atttttccag tgcatacaat tgcgactttt ctgctaaccc tgtttgatca tgaaaaactg
    10981 taaataacgc gtaaacactg gtgataaagc gtgcttcaga tcacatattg cgcatgttcg
    11041 cgcacagcat atttatttac ttggcaaatg atgcctttgc aagtttatga tatttcagtc
    11101 taaaaacaga tactgttt
//
LOCUS       DAZKFU020000150        11086 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZKFU020000150 DAZKFU020000000
VERSION     DAZKFU020000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.151"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            258..761
                     /gene="dps"
                     /locus_tag="ACHOQQ_RS16175"
                     /old_locus_tag="pgaptmp_003235"
                     /gene_synonym="pexB"
     CDS             258..761
                     /gene="dps"
                     /locus_tag="ACHOQQ_RS16175"
                     /old_locus_tag="pgaptmp_003235"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGU4471447.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1165..1911
                     /gene="glnH"
                     /locus_tag="ACHOQQ_RS16180"
                     /old_locus_tag="pgaptmp_003236"
     CDS             1165..1911
                     /gene="glnH"
                     /locus_tag="ACHOQQ_RS16180"
                     /old_locus_tag="pgaptmp_003236"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGU4471448.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2050..2709
                     /gene="glnP"
                     /locus_tag="ACHOQQ_RS16185"
                     /old_locus_tag="pgaptmp_003237"
     CDS             2050..2709
                     /gene="glnP"
                     /locus_tag="ACHOQQ_RS16185"
                     /old_locus_tag="pgaptmp_003237"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGU4471449.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2706..3428
                     /gene="glnQ"
                     /locus_tag="ACHOQQ_RS16190"
                     /old_locus_tag="pgaptmp_003238"
     CDS             2706..3428
                     /gene="glnQ"
                     /locus_tag="ACHOQQ_RS16190"
                     /old_locus_tag="pgaptmp_003238"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGU4471450.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3689..5914
                     /gene="ybiO"
                     /locus_tag="ACHOQQ_RS16195"
                     /old_locus_tag="pgaptmp_003239"
     CDS             3689..5914
                     /gene="ybiO"
                     /locus_tag="ACHOQQ_RS16195"
                     /old_locus_tag="pgaptmp_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGU4471451.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(5911..6837)
                     /gene="rlmF"
                     /locus_tag="ACHOQQ_RS16200"
                     /old_locus_tag="pgaptmp_003240"
     CDS             complement(5911..6837)
                     /gene="rlmF"
                     /locus_tag="ACHOQQ_RS16200"
                     /old_locus_tag="pgaptmp_003240"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGU4471452.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7113..7373
                     /gene="mcbA"
                     /locus_tag="ACHOQQ_RS16205"
                     /old_locus_tag="pgaptmp_003241"
     CDS             7113..7373
                     /gene="mcbA"
                     /locus_tag="ACHOQQ_RS16205"
                     /old_locus_tag="pgaptmp_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGU4471453.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7638..9920
                     /locus_tag="ACHOQQ_RS16210"
                     /old_locus_tag="pgaptmp_003242"
     CDS             7638..9920
                     /locus_tag="ACHOQQ_RS16210"
                     /old_locus_tag="pgaptmp_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGU4471454.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAARLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9962..10639
                     /gene="ybiX"
                     /locus_tag="ACHOQQ_RS16215"
                     /old_locus_tag="pgaptmp_003243"
     CDS             9962..10639
                     /gene="ybiX"
                     /locus_tag="ACHOQQ_RS16215"
                     /old_locus_tag="pgaptmp_003243"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGU4471455.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10713..10979
                     /gene="ybiI"
                     /locus_tag="ACHOQQ_RS16220"
                     /old_locus_tag="pgaptmp_003244"
     CDS             10713..10979
                     /gene="ybiI"
                     /locus_tag="ACHOQQ_RS16220"
                     /old_locus_tag="pgaptmp_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGU4471456.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
BASE COUNT         2716 a         2879 c         2795 g         2696 t
ORIGIN      
        1 ctcgctactt ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc
       61 cgaaaattcc tggcgagcaa ataaataaga attgttctta tcaatatatc taactcattg
      121 aatctttatt agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca
      181 gaatagcgga acacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat
      241 caagaggata tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc
      301 tttatacccg caacgatgtc tctgacagcg agaaaaaagc aacagtagag ttgctgaatc
      361 gtcaggttat ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc
      421 gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg cttccgcact gcactgatcg
      481 atcatctgga taccatggca gaacgtgcag tgcagctggg cggtgtagct ctggggacca
      541 ctcaagttat caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc
      601 aggatcacct gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag
      661 cgattggcga agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc
      721 tggataaatt cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca
      781 gaactttagt acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagcccg
      841 cctcaacgca cttatttagt gcatccatct gctatcccca gctgattaag taaatttttt
      901 gtatccacat catcacacaa tcgttacata aagattgttt tttcatcagg ttttacgcta
      961 aataatcact gtgttgagtg cacaatttta gcgcaccagt ttggtgcccc agaatggtgc
     1021 atcttcaggg tattgcccta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca
     1081 atgagttgca aaactggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc
     1141 cgtggataga aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg
     1201 accctggctt ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat
     1261 accgccttcg ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat
     1321 ctgtgggctg ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc
     1381 agtgggatca ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc
     1441 atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg
     1501 ttagtgatgg tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa
     1561 gtggttgctg tgaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact
     1621 aaagatctgc gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc
     1681 gcagacgccg ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac
     1741 ggtcagttca aagcggtagg tgactctctg gaagcgcagc aatacggtat agcgttcccg
     1801 aaaggtagcg acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac
     1861 ggaacttaca acgaaatcta caaaaaatgg ttcggtactg aaccgaaata ataacgctac
     1921 acctgtaaag cgcactggca gttccctctc ccctctgggg agaggattag ggtgaggggc
     1981 gcaaacccgc tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag
     2041 gaacaacata tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa
     2101 ggtgccaaaa tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga
     2161 ttgctggcag gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc
     2221 tttattgaag tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ttatttcgcc
     2281 ctgccgatgg cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc
     2341 atgatcaact ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctatccac
     2401 aaaggttttc gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac
     2461 gtcattttac cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc
     2521 agcattaaag acacctcgct gtttattgtt atcggcgtgg cggaactgac ccgtcagggg
     2581 caagaaatta ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc
     2641 tatctgatta ttacgctggt gctgagcttt attctgcgtc gtctggaaag aaggatgaaa
     2701 atcctgtgat tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgctgcaca
     2761 atatcgattt gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg
     2821 gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga
     2881 ttgtcgatgg cctgaaggtt aacgatccga aagttgacga gcgcctgatt cgccaggaag
     2941 caggtatggt gttccagcag ttttaccttt tcccgcatct gacggcgctg gaaaacgtta
     3001 tgtttggccc gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcacgtg
     3061 agctgctggc gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg
     3121 gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc
     3181 tgtttgatga accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta
     3241 tgcaggatct ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg
     3301 ccgagaaagt agcttcgcgc ctgatcttca ttgataaagg ccggattgcg gaagatggcg
     3361 atccgcaggt gttgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg
     3421 tctcttaata agacacattg cctgatgcgc tacgcttatc aggcctacag gatatctggc
     3481 aacttattaa aattgcatga acttgtagga cggataaggc gttcacgccg catccggcaa
     3541 aaaagcccgc acgttgtcag caacctgctt aatatccctt cctccctttc acccgaaagg
     3601 gaggcacacc agattcctct catttaaaat cgccccttcc ccagcatcta tacttatctt
     3661 tttgctctgt tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct
     3721 gctgggcgca cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga
     3781 ctcaacgact gaaccggccc cggaaccgga tatcgaacaa aaaaaagcgg cctatggcgc
     3841 actggcggat gtgctggata atgacacctc gcgtaaagag ttgatcgacc agttgcgcac
     3901 cgtcgccgcg acgccacctg ctgaaccggt cccgaagatc gtgccgccga cgctggttga
     3961 agagcaaacc gtgctgcaaa aggtcaccga agtcagccgc cattatggtg aagccctttc
     4021 cgcccgcttc gggcaacttt atcgcaatat caccggctcc ccgcataagc cgtttaatcc
     4081 gcaaaccttc agcaatgcgc tgacccattt ttcaatgtta gcggttttag tgtttggttt
     4141 ttactggctg attcgcctgt gcgcactgcc gctgtatcgc aaaatgggtc agtgggcgcg
     4201 gcaaaaaaat cgtgagcgca gtaactggtt gcagcttccg gcgatgatta tcggggcgtt
     4261 tattatcgac ctgctgttac tggccctgac attatttgtc ggccaggtat taagcgacaa
     4321 cctgaatgca ggcagtcgca ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc
     4381 cctgattgaa tttttcaaag ccgtactacg cctgattttt tgcccaaacg tggcggaact
     4441 gcgcccgttc acgattcagg acgagagcgc ccgttactgg agtcgtcgcc tgagctggtt
     4501 aagcagtttg ataggttatg gcctgattgt ggccgtgccg attatctcta atcaggtgaa
     4561 tgtacaaatt ggcgcgctgg cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt
     4621 gtacctgatc tttcgtaata aaaaagagat tacccagcat ttgctcaact ttgcggagca
     4681 ttcgctggcc tttttcagcc tgtttatccg cgcctttgcg ctggtgtggc actggctggc
     4741 aagcgcctat tttatcgtgc tgtttttctt ttcgttgttc gatccgggca atagcctgaa
     4801 atttatgatg ggtgcaacgg tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc
     4861 cggtatgttt tcccgctggc tggcaaaaac catcactctc tcgccacata ctcagcgtaa
     4921 ctatccggag ttgcaaaaac ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg
     4981 tattctgacc gtctgcgtgg cggtaatgct gctgttgagc gcatggggat tgttcgattt
     5041 ctggaactgg ctgcaaaacg gcgcggggca gaaaaccgta gatatcctga tccgtatcgc
     5101 actcattctt ttcttctcgg cggttggctg gacggtgctc gccagtttga tcgaaaaccg
     5161 gctggcttcg gatattcatg gccgcccgct acccagcgcc cgcacgcgta ccctgcttac
     5221 gctgtttcgt aacgcgctgg cggtgattat cagtaccatc accatcatga ttgtgttgtc
     5281 ggaaatcggc gtcaatatcg cgccattgct ggcaggtgcc ggggcattag gtctggctat
     5341 ctcgtttggt tcgcaaacgc tggtgaaaga tattatcacc ggggtattta ttcagtttga
     5401 aaacggcatg aacactggag atttggtgac tattgggccg ttgaccggca ctgtggaacg
     5461 gatgtcgatt cgctccgtgg gcgtacgcca ggataccggg gcgtaccaca tcattccgtg
     5521 gtcttcgatt accacctttg ccaactttgt ccgcggcatt ggttcggtag tggcaaacta
     5581 cgatgttgat cgccatgaag atgctgataa agccaatcag gcactgaaag atgcggtagc
     5641 ggaattaatg gaaaacgaag aaattcgcgg gctgattatt ggtgaaccga attttgccgg
     5701 gattgtcggc ttaagcaata ccgcgtttac actgcgtgtt tcgttcacta cgctgccgct
     5761 caaacagtgg acggtacgct ttgccctcga cagccaggtg aaaaaacatt tcgacctggc
     5821 gggcgttcgc gcgccagtgc agacttatca ggtgctgcct gctccgggcg cgaccccggc
     5881 tgaaccgtta ccgccggggg agccaacgct ttaacgctgg cgattgacaa aacggcggcg
     5941 ctgctcgtcg ttcataaagg tccaggcaat aaagcgactc tgcttttgcc cctgggccat
     6001 ctctttttta accaccttca ccgcgcccac gtccgtcaga gcacgataca acggcggtaa
     6061 gttttcacca cgagatacca gtgaagtaaa ccacatcacc tgcttcgcga agcctttgct
     6121 ctcttcaatc atctttttga taaaggtgac ttcaccgcct tcacaccaca actcctgttg
     6181 ctggccgcca aagttcagtg catcgtcttt gttcagcccc aggttacggc gtttacgctc
     6241 actacctgcc cgcgccgcag cggcagaatc gtggaatggc gggttacaca aggtcgcgtc
     6301 gtattgctcg tttttatgga tgatgccgtt aaaaatcgcc ccactctctt tttgccgacg
     6361 cagacgaatg gcgcgattaa gccccggatt agcactgatg atcgcctgcg cgctgcttaa
     6421 cgcctggctg ctggtttcac taccggtaaa acgccagcca tattcatgta cgccaatcag
     6481 cggataaata cagttcgcac caacgccgat atccagaatg ctggcattgg ctggaattgt
     6541 tccgcttgct tctgccagta aatcggcaag gtgatgaata taatccgccc ggcccggtac
     6601 tggcgggcag agaaaaccgt cggggatatc ccagttcgct acggcgtaaa aatgggccag
     6661 caacgcctta ttgagcgcct tcaccgccag cggattggca aagtctacgc tttgctcccc
     6721 tgcgggtgta agcgtgagga attgcctgag ttcaggattg acctgacaaa gcgtggcgag
     6781 atcgtagcgg ctgtgatgac ggttgcgcgg atgcaacccc ggtttctggg cggacatagc
     6841 attctccttt aagcagcggc gtaagatacc cgcataacgc gacagggtaa atatctggct
     6901 gcgtttatca tttctaaaaa ccttaacatg gctatggttt atggcaattc atcataaaaa
     6961 tcagcatcat gcatgtcatt gatttgtctt attttgtaaa aatatatcgt tcaaaaaata
     7021 agcgtatctc tgtcaatttt ttgcgtcatt ttcatcgccc aggcgaccta cacttatcag
     7081 gcactacccg ttgtatttat gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg
     7141 ccaccgtcct gagcggaatc tctctcacgg cttatgccgc gcaaccgatg agtaacctgg
     7201 acagcggtca gctacggccc gccggtaccg tttcggcaac tggcgcatca aacctaagcg
     7261 atctggagga taaactggca gaaaaagcgc gcgagcaagg cgcgaaaggt tatgtcatta
     7321 attccgcagg cggaaatgac cagatgttcg gtactgcaac catctacaaa taaccccacc
     7381 aacgtaactg cattcgcctc tggatgcagt tttcgcctgt tttgtaatca aaaaacatca
     7441 aatatgaacg caatgtaaat aaatgtattt ctttttcgcg caatgggtga tagaaaatcg
     7501 cgccaagtga taatgcttat caaaattatt atcacttgca cgaacactat cacgagatta
     7561 acagtggcat cgcatccgca gagaggcttt ctcgtggcag tgaaaatttc aacatataag
     7621 aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc tgccagacaa tttcattcgc
     7681 tcacgttctt tgccggtctt tgtattggca tcacgcctgt ggctcaggca ctcgccgccg
     7741 aagggcaaac taacgcggat gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg
     7801 cgccacaaca atctgcagat ccgaaattct cgcgtccggt agcggatact acccgcacga
     7861 tgacggtgat ttctgaacaa gtgattaaag atcagggcgc aaccaacctt accgacgcac
     7921 tcaaaaacgt ccccggcgtg ggtgcgtttt ttgcgggtga gaacggtaac tccaccactg
     7981 gcgacgccat ttatatgcgc ggtgctgata cctctaacag tatttatatt gatggcattc
     8041 gcgatatcgg cagcgtctcg cgcgacacct tcaataccga gcaggtcgaa gtgattaaag
     8101 ggccgtccgg caccgactac gggcgcagcg caccgacagg ctcgatcaat atgatcagca
     8161 agcagccgcg caatgattcc ggcattgacg cctccgccag tattggtagc gcctggttcc
     8221 gccgcggcac gctggacgtc aatcaggtca ttggtgatac caccgcggcg cgcctgaatg
     8281 tgatgggcga aaaaacgcac gatgccggac gcgacaaagt caaaaatgag cgttacggcg
     8341 tcgccccttc tgtcgctttt ggccttggta cagcgaatcg tttgtatctt aattatctgc
     8401 atgtcaccca gcacaacacg ccagacggcg gcattccaac catcggtttg ccgggctatt
     8461 ctgccccatc tgcgggaacg gcggccctga atcattccgg aaaagttgat acgcataact
     8521 tttacggcac ggattccgat tacgacgatt cgaccaccga caccgctacc atgcgttttg
     8581 agcacgacat caacgataac accaccattc gcaacactac ccgttggtcg cgcgtgaagc
     8641 aggattacct gatgacggcg attatgggtg gggcgtcgaa tattacccag cccaccagcg
     8701 atgtgaatag ctggacgtgg tcacgcacgg cgaataccaa agatgtgagt aataaaattc
     8761 tcaccaacca gaccaacctg acctcgacgt tctataccgg ttctatcggt catgatgtca
     8821 gtaccggcgt ggaatttacc cgtgaaacgc agactaacta cggcgttaat ccggtgacgt
     8881 taccggcggt aaatatttat catcctgaca gcagcattca tcccggcgga ctgacgcgca
     8941 acggcgcaaa cgccaatggt cagacggata ccttcgcaat ttacgccttc gatacgctgc
     9001 aaatcacccg tgattttgag ctgaacggcg ggatccgtct ggataattat catactgaat
     9061 atgacagtgc caccgcctgc ggcggcagcg gacgcggtgc catcacctgc ccagctggtg
     9121 tggcaaaagg ttctccagtc accaccgtcg acaccgccaa gtcgggcaat ctggtgaact
     9181 ggaaagccgg ggcgctgtat cacctgacgg aaaacggcaa tgtctatatt aactatgccg
     9241 tttcccagca gcctccgggc ggcaacaact tcgcccttgc gcagtctggc agtggtaaca
     9301 gtgccaaccg caccgatttt aaaccgcaaa aagccaacac cagcgagatt ggcaccaaat
     9361 ggcaggttct ggataaacgc ctgttgctca ccgccgcgct gttccgtact gatatcgaaa
     9421 atgaagttga gcaaaatgat gacgggactt actcgcaata cggtaagaaa cgcgtcgaag
     9481 gctatgagat atccgtggca gggaatatca ctcccgcgtg gcaggtgatt ggcggctata
     9541 cccagcaaaa agcaaccatc aaaaacggca aagatgttgc ccaggatggt tcctcatcgc
     9601 tgccgtatac cccggagcac gccttcacct tatggagcca atatcaggca accgatgata
     9661 tttctgtggg tgcgggcgca cgctatatcg gcagtatgca taaaggttca gacggcgcgg
     9721 tgggaacgcc agcgtttacc gaaggttact gggtcgccga tgccaaactg gggtatcggg
     9781 ttaatcgcaa tctcgacttc cagctaaacg tctacaacct gtttgatacc gattacgtcg
     9841 cctcaatcaa taagagcggc taccgttatc acccgggcga gccaagaacc ttcttgctca
     9901 cagccaatat gcatttctga ttcagatgtg gggcgcaggc cccacttttt ggagaacttg
     9961 tatgatgtac cacattcccg gcgtgttatc gccacaggac gtcgcccgtt ttcgcgaaca
    10021 actggaacaa gccgaatggg tggatggacg cgtcaccact ggcgcacaag gtgcgcaagt
    10081 taagaacaat caacaggtcg acacccgcag cacgttatac gccgccctgc aaaatgaggt
    10141 gctgaacgcg gttaaccaac atgctttgtt ctttgccgcg gccttgccgc gtaccctttc
    10201 cacgccgctg tttaatcgct atcagaacaa tgaaacctat ggttttcatg tggatggcgc
    10261 agtacgcagc catccacaaa acggctggat gcgcactgac ctttctgcca cgctgttttt
    10321 aagcgatcca caaagctacg acggcggcga actggtcgtt aatgacacct tcggacaaca
    10381 tcgggtaaaa ctcccggcag gcgatctggt gttgtatccc tccagcagcc tgcattgcgt
    10441 gacacccgta acccgcggcg tacgagtggc atcatttatg tggatccagt cgatgatccg
    10501 tgatgataaa aagcgcgcca tgctgtttga actggacaac aatattcagt cgctgaaaag
    10561 ccgctacggc gaaagtgaag agatcctgtc gctgctcaat ctttatcata atctgctgcg
    10621 ggaatggtcg gagatctgac gcttgaactg cccgagttcg tccctatatt agctacactt
    10681 aactgtacaa gcattgatat ggggaggacg atatggcatc cggttgggct aacgatgacg
    10741 ccgtcaacga acagatcaac agtacaattg aagatgcgat tgcccgcgct cggggtgaaa
    10801 ttccgcgtgg cgaaagcctg tatgaatgtg aagagtgcgg tgcccccatc ccgcaagccc
    10861 gtcgggaagc cattcctggc gtgcgcttat gtattcattg tcagcaggag aaagatttac
    10921 aaaaaccagc ttatacagga tataatcgca gaggttcgaa agacagccag ttacgttaac
    10981 tacaactggc gagatgtata gcgagtataa tttgtatttt gcgtggcatt ccgtgccttt
    11041 aacgctgctg tgactgccac gctgcataaa acaagtccat aagtac
//
LOCUS       DAZKFU020000151        10968 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZKFU020000151 DAZKFU020000000
VERSION     DAZKFU020000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.152"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1134
                     /locus_tag="ACHOQQ_RS16225"
                     /old_locus_tag="pgaptmp_003245"
     CDS             <1..1134
                     /locus_tag="ACHOQQ_RS16225"
                     /old_locus_tag="pgaptmp_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4471457.1"
                     /translation="EAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESWYLRSENAY
                     RAEVPLYASMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIVRG
                     ATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDSSRAGTVRDDA
                     GSLGGYLNLIHNASGLWADIVAQGTRHSMKASSDNNDFRVRGRGWLGSLETGLPFSIT
                     DNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSS
                     RDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQNGTSLD
                     LQAGLEARVRENITLGVQAGYVHSVSGSSAEGYNGQATLNVTF"
     gene            1255..3771
                     /locus_tag="ACHOQQ_RS16230"
                     /old_locus_tag="pgaptmp_003246"
     CDS             1255..3771
                     /locus_tag="ACHOQQ_RS16230"
                     /old_locus_tag="pgaptmp_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471458.1"
                     /translation="MLQIVGALILLITGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     IFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            3847..4302
                     /locus_tag="ACHOQQ_RS16235"
                     /old_locus_tag="pgaptmp_003247"
     CDS             3847..4302
                     /locus_tag="ACHOQQ_RS16235"
                     /old_locus_tag="pgaptmp_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HGU4471459.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            4381..4614
                     /locus_tag="ACHOQQ_RS16240"
                     /old_locus_tag="pgaptmp_003248"
     CDS             4381..4614
                     /locus_tag="ACHOQQ_RS16240"
                     /old_locus_tag="pgaptmp_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU4471460.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            4714..5532
                     /locus_tag="ACHOQQ_RS16245"
                     /old_locus_tag="pgaptmp_003249"
     CDS             4714..5532
                     /locus_tag="ACHOQQ_RS16245"
                     /old_locus_tag="pgaptmp_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4471461.1"
                     /translation="MRLASRFGYANQIRRDHPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            5587..6072
                     /locus_tag="ACHOQQ_RS16250"
                     /old_locus_tag="pgaptmp_003250"
     CDS             5587..6072
                     /locus_tag="ACHOQQ_RS16250"
                     /old_locus_tag="pgaptmp_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4471462.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVASLVPDEQRISFWPQHF
                     GLIPQWVTLESRVFGWMDRLYEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            6088..6564
                     /locus_tag="ACHOQQ_RS16255"
                     /old_locus_tag="pgaptmp_003251"
     CDS             6088..6564
                     /locus_tag="ACHOQQ_RS16255"
                     /old_locus_tag="pgaptmp_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU4471463.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            6627..6848
                     /locus_tag="ACHOQQ_RS16260"
                     /old_locus_tag="pgaptmp_003252"
     CDS             6627..6848
                     /locus_tag="ACHOQQ_RS16260"
                     /old_locus_tag="pgaptmp_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4471464.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            6867..7511
                     /locus_tag="ACHOQQ_RS16265"
                     /old_locus_tag="pgaptmp_003253"
     CDS             6867..7511
                     /locus_tag="ACHOQQ_RS16265"
                     /old_locus_tag="pgaptmp_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HGU4471465.1"
                     /translation="MSRIITSTVYTLNELSATAREKARDGYRQHHADSNWYENVYEDF
                     RKVCDIFGIDLRQRVFQLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQSATARIIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEIHHRGRYCHPYSMDITVTRNSPTGQIMT
                     TDAEAAVSEALRDLAFWLYRQLENEYDWLTSDATVDEALLINEYTFTEAGLRAG"
     gene            7561..7929
                     /locus_tag="ACHOQQ_RS16270"
                     /old_locus_tag="pgaptmp_003254"
     CDS             7561..7929
                     /locus_tag="ACHOQQ_RS16270"
                     /old_locus_tag="pgaptmp_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4471466.1"
                     /translation="MSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCDAD
                     TLGSCGYVYIAIYPTPGTKT"
     gene            8019..8396
                     /locus_tag="ACHOQQ_RS16275"
                     /old_locus_tag="pgaptmp_003255"
     CDS             8019..8396
                     /locus_tag="ACHOQQ_RS16275"
                     /old_locus_tag="pgaptmp_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4471467.1"
                     /translation="MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     NERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            8393..8881
                     /locus_tag="ACHOQQ_RS16280"
                     /old_locus_tag="pgaptmp_003256"
     CDS             8393..8881
                     /locus_tag="ACHOQQ_RS16280"
                     /old_locus_tag="pgaptmp_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4471468.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINKVAENGYS
                     LRVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            8893..9090
                     /locus_tag="ACHOQQ_RS16285"
                     /old_locus_tag="pgaptmp_003257"
     CDS             8893..9090
                     /locus_tag="ACHOQQ_RS16285"
                     /old_locus_tag="pgaptmp_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4471469.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            9175..10041
                     /locus_tag="ACHOQQ_RS16290"
                     /old_locus_tag="pgaptmp_003258"
     CDS             9175..10041
                     /locus_tag="ACHOQQ_RS16290"
                     /old_locus_tag="pgaptmp_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4471470.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAIKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISAANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKENYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            10113..10376
                     /locus_tag="ACHOQQ_RS16295"
                     /old_locus_tag="pgaptmp_003259"
     CDS             10113..10376
                     /locus_tag="ACHOQQ_RS16295"
                     /old_locus_tag="pgaptmp_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4471471.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            10373..10699
                     /locus_tag="ACHOQQ_RS16300"
                     /old_locus_tag="pgaptmp_003260"
     CDS             10373..10699
                     /locus_tag="ACHOQQ_RS16300"
                     /old_locus_tag="pgaptmp_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4471472.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
BASE COUNT         2757 a         2896 c         2856 g         2459 t
ORIGIN      
        1 gaagccatta acggtgccac cacggaggaa ggggcctttg tccaggggaa caggctgcag
       61 gccggggcct ttaactactc cctcaaccgg gacagtgatg agagctggta tctgcgcagt
      121 gaaaatgctt atcgtgcaga agtccccctg tatgcctcca tgctgacaca ggcaatggac
      181 tatgaccgga ttctggcagg ctcccgcagc catcagaccg gtgtaaatgg tgaaaacaac
      241 agcgtccgtc tcagcattca gggcggccat ctcggtcacg ataacaacgg cggtattgtc
      301 cgtggggcca cgccggaaag cagcggcagc tatggcttcg tccgtcttga gggtgacctg
      361 ctcagaacag aggtcgccgg tatgtctctg acgacagggg tgtatggtgc tgcaggccat
      421 tcttccgttg atgttaagga tgatgacagc tcccgtgccg gcacggtccg ggatgatgcc
      481 ggcagcctgg gcggatacct gaatctgata cacaacgcct ccggactgtg ggctgacatt
      541 gtggcccagg gaacccgcca cagcatgaaa gcctcatcgg acaataacga cttccgcgtc
      601 cggggccggg gctggctggg ttcactggaa accggtctgc ccttcagtat cactgacaat
      661 ctgatgctgg agccgcaact gcagtacacc tggcagggac tctccctgga tgacggtaag
      721 gacaacgccg gttatgtgaa gttcgggcat ggcagtgcac aacatgtgcg tgccggcttc
      781 cgtctgggca gccacaacga tatgaacttt ggtaaaggca cctcatcccg tgacaccctg
      841 cgcggcagtg ccaaacacag tgtgcgtgaa ctgccggtga actggtgggt acagccttct
      901 gttatccgca ccttcagttc ccggggggac atgagcatgg gtacagccgc agccggcagt
      961 aacatgacgt tctcaccgtc acagaatggt acgtcactgg acctgcaggc cggactggaa
     1021 gcccgtgtcc gggaaaacat caccctgggc gttcaggctg gttatgtcca cagcgtcagc
     1081 ggcagcagcg ctgaaggcta taacggtcag gccacgctga atgtgacctt ctgacagaac
     1141 catcgcctct ctgtggtccc ggtcatcatg accgggaccc ggaccggcgc aacggatatt
     1201 caacgccaca ttcgctggca ttaacaataa catgatattc atcacggagt gactatgtta
     1261 cagatagtcg gcgcgctgat cctgctgatc acaggatttg ccattcttcg ccttttgttc
     1321 agagcattaa tcagcacggc ttctgcgctg gcagggctca tattgctgtg tctgttcggc
     1381 ccggccttac tggctggcta tatcaccgaa cgcataaccc ggttgttcca tattcgctgg
     1441 ctggcaggcg tatttctgac gattgccgga atgatcatca gcttcatgtg gggacttgat
     1501 ggtaaacata tcgcgctgga ggctcacacc tttgactctg tgaaatttat tctgaccact
     1561 gctctcgccg gtggtctgct ggctgttccc ctgcagatca aaaacattca gcagaacggg
     1621 atcacaccag aagatatcag caaggaaatt aacgggtatt actgctgttt ttatactgcc
     1681 tttttcctta tggcgtgttc tgcatgcgca ccattgatcg cgttacagta cgatatttca
     1741 ccgtcactga tgtggtgggg cgggttgttg tactggctgg ctgcattagt gacgctgcta
     1801 tgggcggcca gccagatcca ggcgctgaaa aaactgacct gtgccatcag ccagacactg
     1861 gaagaacaac cggtgctcaa cagtaaatcg tggctgacca gtttgcaaaa cgattacagc
     1921 cttcctgact cactgacgga gcgcatctgg ctgacgctca tttctcaacg gatttcccgg
     1981 ggagagctga gggaatttga actggcagac ggaaactggt tactgaacaa tgcctggtat
     2041 gaaagaaaca tggcagggtt taacgaacag ttgaaagaga acctgtcatt cacacctgat
     2101 gaactgaaaa cgctcttccg gaaccgcctg aatttatcac cggaagcgaa tgacgatttt
     2161 ctcgatcgtt gcctggacgg cggtgactgg tatccctttt cagaaggtcg ccgttttgta
     2221 tcattccatc acgtggatga gcttcgtatc tgtgcctcct gcgggctgac agaagtacat
     2281 catgccccgg aaaatcataa gccggatccg gaatggtact gctcctctct ttgtcgcgaa
     2341 acagaaacac tgtgtcagga aatttatgaa cgcccttaca acagctttat ttccgatgca
     2401 acggcgaatg gtctgattct catgaaactg ccggaaacct ggagtacaaa tgagaaaatg
     2461 tttgcttccg gagggcaggg acatgggttt gccgctgaac ggggaaacca tattgtcgac
     2521 agagtccgtc tgaaaaacgc acggatcctc ggtgataata atgccagaaa tggagcagac
     2581 agactggtca gcggaacaga aatccagacg aaatattgtt caactgcagc ccgtagcgtc
     2641 ggtgcggcat tcgacggaca aaacggacag tatcgttaca tgggaaataa tggccccatg
     2701 caactggaag tcccccgtga tcagtatgcc ggcgctgttg aaaccatgag gaataagatc
     2761 cgcgaaggta aagtacccgg tgtaaccgat cccgcagaag cgtcccggct gattcgtcgg
     2821 ggacatctga cttatactca ggcccgtaat atcacccggt tcgggaccat cgaatcggtc
     2881 acttatgata ttgccgaggg gtcggttgtc agtctggcgg ccggagggat cagttttgcc
     2941 ctgacggcat cgatcttctg gctcagcacc ggcgatcgcg atgctgccct gcagacagct
     3001 gctgtccagg caggaaaaac cttcacccgc acactggctg tctacgtcac aacccagcaa
     3061 cttcaccggc tcactgttgt tcagggtatg ctgaagcata ttgatttttc gacggccagc
     3121 ccgactgtcc ggcaggcgct tcagaagggg accggtgcag gaaatatcag tgccctgaac
     3181 aaagtgatga agggtacgct ggtgacatct ctggcactgg tagctgtcac aaccggccct
     3241 gacatgatca aaatgttgcg gggacggatc tccggtgcgc agttcatcag gaatcttgcc
     3301 gtggcatctt ccggtgtggc aggtggtgct gtcgggtcag tggcgggcgg gatattgttc
     3361 agtccactgg gaccatttgg tgcactgaca gggcgtgtgg ttggcggtgt tctgggggga
     3421 atgattgcct ccgctgtatc aggaaaaatt gccggagcgc tggttgaaga agatcgcgtc
     3481 aaaattctgg caatgattca ggagcaggtg acatggcttg ccggcagttt cctgctgacc
     3541 ggacatgaga ttgaaaatct gaacgcgaat ctggcccgtg ttatcgatca gaatgctctg
     3601 gagatcattt tcgccgccgg tatacaacaa cgggccgcga ccaatatgtt aatcaaacca
     3661 ctggtggtca gtatcatcag gcaacgcccc gtcatggaat atgatgcatc ccatctcggc
     3721 aatatggtta accgactgga agaagcatta cccccggagt taccggcata acggctattc
     3781 tgcgcctttt acgctttatc acctgatttg tcagtgtcat cgtcatgact cagagaggta
     3841 aataccatga ttcacctgtt caaaacctgc atgattaccg ccttcattct ggggttaacg
     3901 tggtctgccc cactccgggc acaggatcaa cgttacatca gtatacgcaa tacagatacg
     3961 atatggctcc cgggaaatat ttgtgcttac cagttccggc tggataatgg cggaaacgat
     4021 gaaggatttg gccccctcac catcactctg caactcaaag acaaatatgg tcagacgctg
     4081 gtgaccagaa aaatggaaac ggaagctttt ggtgacagta atgccacgcg aaccacagac
     4141 gcatttctgg aaacggagtg cgtggaaaat gtcgccacaa ccgaaatcat taaagcaact
     4201 gaagaaagta acggccatcg tgtcagtctg ccgttatcgg ttttcaatcc ccaggactac
     4261 catccactgc tgattaccgt ttccggaaaa aacgttaact gatccctgaa gtcagtttca
     4321 tgcttcagcc tctgtcacaa cgcgtggcag aggcttttta tttatcagga gaaaacagat
     4381 atgccgggtt acacagagta tgtcctggct gaaggatctt tctcatacgg gcaggcggtt
     4441 gctgtcataa ccgcttaccg caatgtgttt atccaggatg acccgggaat gcatttccgt
     4501 cgggttatcc gcaacgcgga aggtcagcga cgctggcgct gccggaattc tgaacccgat
     4561 gccggaaagg tgcttaatac gcggctcgcc agtgacggtc ttctcagaca ataaacgtca
     4621 ttcatttcac ccatcaggcc gcgtcttctc cgggagacgt ggctttttca tttattacac
     4681 cagtaaatct taaccaccga taaggagcaa attatgcgac tagccagtcg ttttggttat
     4741 gcgaaccaga tacgccgtga ccatccgctg acacacgaag aactgatgca ctatgtgccg
     4801 gggattttcg gggaagataa acacacgtcc cgaagccaga actatacgta catccccacc
     4861 atcaccgtac tggaaagcct gcagcgggaa ggctttcagc cattcttcgc ctgccagacc
     4921 cgtgtgcgcg acccgggccg ccggggatac acaaaacaca tgctgcgtct gcggcgggcc
     4981 ggagagataa acggagaaca tgtccctgaa attattctgc tcaactctca tgacggtacc
     5041 tccagctacc agatgctgcc gggttacttc aggttcgtct gccagaacgg atgcgtctgt
     5101 ggtcagtctc tgggggaagt gcgtgttccg caccggggaa atgtggtgga cagagtcatt
     5161 gaaggggctt acgaggtggt gggggttttt gaccggattg aggaaaagcg tgatgccatg
     5221 cagtcgctga ttctgccgcc accggcacgc caggcgctgg cacaggcggc actgacttac
     5281 cgttatggtg acgaacatca gcccgtcacc accgccgaca ttctgacgcc acgacgccgg
     5341 gaggattacg gtaaggacct gtggagtgct tatcagacca ttcaggagaa tatgctgaaa
     5401 ggcggaattt caggtcgcag cgctaaagga aaacgtatcc atacccgtgc cattcacagc
     5461 attgacactg acattaagct caaccgcgca ttgtgggtga tggcggaaac gctgctggag
     5521 agcctgcgct gattcagcca ttaccctgaa aaccacgcat ttatttgtcc tttaaggagt
     5581 catttcatga aaaccatttc acataattcc accacatctt ctgtttccgt aaccgctgca
     5641 tcagggaatg accagcctca gttggttgcc agcctcgtcc ctgatgaaca gcgcatcagc
     5701 ttctggccgc agcattttgg cctcattcca cagtgggtga ccctggagtc ccgggtcttc
     5761 ggctggatgg accgtctgta tgaagactac tgcggtggta tctggaatct gtacaccctg
     5821 aacaacggcg gggcatttat ggcccccgaa ccggatgacg atgatggcga aaactgggta
     5881 ctgttcaatg ccatgaacgg taaccgcgct gaaatgagcc cggaagccgc cggcattgcc
     5941 gcctgtctga tgacgtacag tcatcatgcc tgtcgtacgg agtgttatgc catgacggtc
     6001 cattattacc ggctgcggga ttacgccctg cagcatccgg aatgcagcgc cattatgcgc
     6061 atcatcgact gaatggagag aagcacaatg caacaacttt cctttctgcc cggagagatg
     6121 acgcccggcg agcgcagcct cattcagcgg gccctgaaaa ccctggaccg ccatcttcat
     6181 gaacccggcg tggccttcac ctccacccgt gcggcacggg aatggctgat tctgaacatg
     6241 gcgggactgg agcgtgaaga attccgggtg ctgtatctga ataaccagaa tcaactgatt
     6301 gccggtgaaa ccctcttcac cggcaccatc aaccgcacgg aagtccatcc ccgggaagtg
     6361 attaaacgcg ccctgtacca caatgccgct gccgtggtgc tggcgcacaa tcacccgtcc
     6421 ggtgaagtca cacccagcaa ggcagaccgc cttatcacgg aacgtctggt acaggcactg
     6481 ggcctggtgg atatccgggt gccggaccat ctgatagtcg gtggcaacca ggttttctcc
     6541 tttgccgaac atggtctgct ttaacccgtc acaaccacat cacgcttgtt ttcactttta
     6601 tcttctgtct tcagaggtat cacactatga aaatcatcac ccgtggtgaa gccatgcgta
     6661 ttcaccaaca acatccggca tcccgtcttt ttcctttctg taccggcaaa taccgctggc
     6721 acggcagtgc tgaggcgtat accggtcgtg aagtgcagga tattcccggt gtgctggccg
     6781 tgtttgctga acgccgtaag gacagttttg gcccgtatgt ccggctgatg agcgtcaccc
     6841 tgaactgaat caggacgggc attcagatga gcagaattat cacctccacc gtgtatacac
     6901 tgaatgagct atccgccacc gcacgggaaa aagcccgcga cgggtaccgt cagcatcatg
     6961 ctgacagcaa ctggtatgaa aacgtgtatg aagacttcag gaaggtctgt gacattttcg
     7021 ggattgacct ccgccagcgc gttttccagc tcagcaacgg caggtttatg gaagagccgt
     7081 gcatctggtt cagcggattc tgcagccagg gggacggagc ctgttttgaa ggacgctggc
     7141 actggcagtc agcaacagcc aggataatcc gggagtatgc cccgcaggac cgtgagcttc
     7201 atcgtatcgc ggatgccctg caggcggtgc agaagcgtaa cttctggcaa ctgcaggctg
     7261 aaatccacca ccggggacgc tactgccatc cgtacagcat ggatatcacc gtcacccgga
     7321 acagtccgac tggtcagata atgacaaccg atgcggaagc cgctgtcagc gaagccctgc
     7381 gcgacctggc cttctggctg taccgtcaac tggaaaacga gtacgactgg ctgacctcag
     7441 atgccactgt ggatgaggcc cttctcatca acgaatacac ctttaccgaa gccggtctgc
     7501 gcgccggctg acccgtcatc gttaaactat cattcaccga ttttctgtga ggattttatc
     7561 gtgtcagaca cactctccgg gacaacgcat cctgacgaca atcacgaccg cccctggtgg
     7621 ggactcccct gcacagtgac gccctgtttc ggggcacgtc tggtgcagga gggtaaccgg
     7681 ttgcattacc ttgcagaccg cgccgacatc agaggccggt tcagcgatgc ggatgcatac
     7741 catctggacc aagcctttcc gctgctgatg aaacaactgg aactcatgct caccagcggt
     7801 gaactgaatc cccgccatca gcataccgtc acgctgtatg caaaagggct gacctgcgat
     7861 gccgataccc tgggcagttg tggttacgtg tatatcgcca tttatccgac acccggaacg
     7921 aaaacataaa ccaccagaat aaacctctgc tactgcccgg tgcaccacag caccattctc
     7981 ctcaaaattt aatcgctcat atcaggaaaa tcttcattat gaaaacatta cccgacacac
     8041 acgtacgggc ggcatcgcgc tgcccgtctc cggttgaaat ctggcagaca ctgctcaccc
     8101 gactgctgga ccagcactac ggcctcacgc tgaatgacac accgttcgcc aatgaacgtg
     8161 tgattgagca gcatattgag gcaggcattt cactgtgtga tgcggtgaac tttctcgtgg
     8221 aaaaatacgc gctggtacga actgaccagc caggattcag cgcctgtacc cgctctcagt
     8281 taataaacag catcgatatc ctccgggctc gccgggcaac aggcctgatg acccgcgaca
     8341 attacagaat ggtaaataac attacccagg gtaagcatcc ggaggcgcaa caatgaaact
     8401 ggccctgaca ctggaagccg acagcattaa cgtgcaggca ctgaacatgg ggcgcattgt
     8461 cgttgacgtc gatggcattg agctcgctga actgattaac aaagtcgctg aaaacggtta
     8521 ttcactccgt gtggtggacg actccgacca acagtcaacc tgcacaccac caccgttaac
     8581 aacccttacc ggcatacgct gcagcaccgc acatatcacg gaaacagaca acgcctggtt
     8641 gtactcgctg tcacaccaga ccagtgactt cggcgaatca gaatggattc attttacagg
     8701 tagcggatat ctgttacgta ccgatgcgtg gtcatacccg gttctgcggc ttaaacgcct
     8761 ggggctgtca aaaatgttcc gccgtctggt tgtcacactt atccggcgtt atggcgtcag
     8821 tctcattcat ctggatgcca gcgctgaatg cctgccgggt ttacccactt tcgactggta
     8881 accagaaaca acatgaaatc attaaccact gaaacagcgc tggatatcct cattgcgtgg
     8941 ctgcaggaca atatcgactg cgaatcggga attatctttg acaacgatga ggataaaacg
     9001 gattcggcag cactgttgcc ctgtatcgaa caggcccggg aggatgtccg tactcttcgt
     9061 catctgcagc ttctgcacca gaaccggtaa atatcactca ccagacttca ttccactgac
     9121 gccagcctga acagggctgg cgttttcatt tatctgcaaa aaggaatatc gattatgtca
     9181 ggtatcacaa tctcccgtcc tgaagtggtc aacgggcata cggacgttat ctgttccacc
     9241 tcaatcggac acattctggc tgtacgaaag accacactga tgcaaatcga cacacttatc
     9301 cggcaactga cagaaatctc agcaatgaca gaaagtattg gaggtaaaac cgccccggac
     9361 tgggccataa aacagaattt tcgctgcggt tgctggctga tggagaaacc cgaaacagca
     9421 atgaaagcca ttacccggaa tcttgaccgc gaaatctggc gtgacctgat gcaacgttcc
     9481 gggatgctgt cattaatgga tgcacaggca cgtgatacat ggtaccggtc actggaatac
     9541 gataattttc cggaaatcag tgcggcaaac attctgagca catttgaaca actgcaccag
     9601 aataaggatg aggtgtttga gcgaggggtg atcaacctct tcaggggact gagctggaat
     9661 tacaaaacca actcaccctg taaatttggc agtaaaatta tcgtcaacaa cctggtgagg
     9721 tgggaccggt gggggtttca ttttatcacc gggcaacagg cagatcgact tgccgatctg
     9781 gaaagaatgc tacacctgtt cagcggcaaa ccgatccccg acaaccggga aaacatcacc
     9841 cttcgtctgg atgatcatat ccagtctgtt cagggtaaag agaactatga agacgagatg
     9901 ttcagtatta aatactttaa gaaaggctca gtacacatca cgttcaggaa accagaactg
     9961 gttgacaggc ttaatgatat tattgccaga cactacccgg aagccctggc aagcacagtg
    10021 aacaaaagcc gcaaggcata aaagaggtat tttacaattc gcgaatatta actattatta
    10081 attgatagca atcaatcggc gagaaaatca ccatgcctac cagtgtatcc ctcagtcctt
    10141 actttgaaac gtttatccgc gaacaaatag aaagtggtcg gtataacaat acaagtgaag
    10201 tcattcgtgc cggacttcgt gctctggaag aacgggaaca gcagataaag cttgaatcac
    10261 tccagagcgc cgttaccgca ggcatcaaca gcggtgaaag taaaagtgct gaagaggtat
    10321 tcgggcgact gacgcacaaa tacaaaaaga tggcagaagg tgaacagcca atatgaaact
    10381 gactgtttca ccattagcag aacacgatct tgaagctata ggtgactgga tcgctcagga
    10441 taatcctgtc cgtgcaatca gttttaccga agagctttac caacaatgtc tgttgatagc
    10501 cgaatcgcca gtactctaca gagaaagacc cgaattaggg gctgggatca gaggttgttc
    10561 gtatggtcgt taccttctgc tgttcagggt actggatact gaagttcgta tcgaacgtat
    10621 cgttcatggt tcccgggata tcaacagggt acttgcagag ccagacgaca aaccatcaga
    10681 tcatttctgg cagccataaa caaaatctgt gcgttaacgc tccctaacac aggtagccag
    10741 gtattttata aatctctgta ttttgttgtt acttcgattc cgcaattcag catatcgaac
    10801 cagacaaaat gattacagca aagcacaaaa aagagggatt atagcgtcaa aagaattcga
    10861 attgcacatg ggggcctcat tggtggcctc gaaaacaaca aagacaaaac aatcaataca
    10921 aaacaattgg ttatataacc agtttcattc cgagtccggg caccacta
//
LOCUS       DAZKFU020000152        10940 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZKFU020000152 DAZKFU020000000
VERSION     DAZKFU020000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.153"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            316..1656
                     /gene="dcuB"
                     /locus_tag="ACHOQQ_RS16305"
                     /old_locus_tag="pgaptmp_003261"
     CDS             316..1656
                     /gene="dcuB"
                     /locus_tag="ACHOQQ_RS16305"
                     /old_locus_tag="pgaptmp_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGU4471473.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1734..3380
                     /gene="fumB"
                     /locus_tag="ACHOQQ_RS16310"
                     /old_locus_tag="pgaptmp_003262"
     CDS             1734..3380
                     /gene="fumB"
                     /locus_tag="ACHOQQ_RS16310"
                     /old_locus_tag="pgaptmp_003262"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4471474.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3503..4132
                     /gene="yjdF"
                     /locus_tag="ACHOQQ_RS16315"
                     /old_locus_tag="pgaptmp_003263"
     CDS             3503..4132
                     /gene="yjdF"
                     /locus_tag="ACHOQQ_RS16315"
                     /old_locus_tag="pgaptmp_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGU4471475.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4271..5692)
                     /gene="melB"
                     /locus_tag="ACHOQQ_RS16320"
                     /old_locus_tag="pgaptmp_003264"
     CDS             complement(4271..5692)
                     /gene="melB"
                     /locus_tag="ACHOQQ_RS16320"
                     /old_locus_tag="pgaptmp_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGU4471476.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5795..7150)
                     /gene="melA"
                     /locus_tag="ACHOQQ_RS16325"
                     /old_locus_tag="pgaptmp_003265"
     CDS             complement(5795..7150)
                     /gene="melA"
                     /locus_tag="ACHOQQ_RS16325"
                     /old_locus_tag="pgaptmp_003265"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGU4471477.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7433..8341
                     /gene="melR"
                     /locus_tag="ACHOQQ_RS16330"
                     /old_locus_tag="pgaptmp_003266"
     CDS             7433..8341
                     /gene="melR"
                     /locus_tag="ACHOQQ_RS16330"
                     /old_locus_tag="pgaptmp_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGU4471478.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            8540..10807
                     /gene="adiA"
                     /locus_tag="ACHOQQ_RS16335"
                     /old_locus_tag="pgaptmp_003267"
     CDS             8540..10807
                     /gene="adiA"
                     /locus_tag="ACHOQQ_RS16335"
                     /old_locus_tag="pgaptmp_003267"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGU4471479.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2809 a         2904 c         2676 g         2551 t
ORIGIN      
        1 taatgacatc tgccctgaga acaaaaaata gaccgataaa tatcaataag ataacagcaa
       61 acaaaacatt aacatctgcg cagtacaaac tataaaccca tcgccagaga gtctttctct
      121 ctgaaaaagc cgcttatcac agtgcataaa tttgccgctg ctttaatcag ccaatattca
      181 ctgtgaggta tttgctaaag ccggtaacga ccaaacggat atttagtcag gctctgaaaa
      241 cagttcatac aaaacagaac gtgactgtga tctattcagc aaaaatttaa ataggattat
      301 cgcgagggtt cacacatgtt atttactatc caacttatca taatactgat atgtctgttt
      361 tatggtgcca gaaagggtgg tatcgcgctg ggtttattag gcggtatcgg tctggtcatt
      421 ctggtcttcg tcttccacct tcagccaggt aaaccaccag ttgatgtcat gctggttatc
      481 attgcggtgg tggcggcatc ggcgaccttg caagcttcgg gcggtcttga tgtcatgctg
      541 caaattgccg agaagttgct gcgccgcaac ccgaaatatg tctcaattgt cgcgccgttt
      601 gtgacctgta cactgaccat tctttgcggt acgggtcatg tggtttacac cattctgccg
      661 atcatctacg acgtcgccat taagaacaac atccgtccgg aacgtccgat ggcggcaagt
      721 tctatcggtg cacagatggg gattatcgcc agtccggtgt cggttgcggt cgtgtctctg
      781 gttgcaatgc tgggtaatgt cacctttgat ggtcgccatc ttgagttcct cgacctgctg
      841 gcaatcacca ttccatcgac gttaatcggt atcctggcga tcggtatctt cagctggttc
      901 cgcggtaaag atctggataa agacgaagag ttccagaaat tcatctccgt accggaaaac
      961 cgtgagtatg tttacggtga taccgcgacg ctgctcgata aaaaactgcc gaaaagcaac
     1021 tggctggcaa tgtggatttt cctcggtgca atcgctgtag tcgcacttct tggtgctgat
     1081 tcggacctgc gtccatcctt cggcggcaaa ccgctgtcga tggtactggt tattcagatg
     1141 tttatgctgc tgaccggggc gctgattatt atcctgacca aaaccaatcc cgcgtctatc
     1201 tcaaaaaacg aagtcttccg ttccggtatg atcgccatcg tggcggtgta cggtatcgca
     1261 tggatggcag aaaccatgtt cggtgcgcat atgtctgaaa ttcagggcgt actgggtgaa
     1321 atggtgaaag agtatccgtg ggcctatgcc attgttctgc tgctggtttc caagtttgta
     1381 aactctcagg ctgcggcgct ggcggcgatt gttccggtcg cgctggcgat cggcgttgat
     1441 ccggcataca tcgtggcttc agcaccggct tgctacggtt attacatcct gccgacttat
     1501 ccgagcgatc tggcagcgat tcagtttgac cgttccggca ccacccacat cggtcgcttc
     1561 gtcatcaacc acagctttat tctgccgggg ttgattggtg tgagcgtatc gtgcgtcttc
     1621 ggctggatct tcgccgcgat gtacgggttc ttataaatgc actttgcgtg ccgcccgtga
     1681 ctacgcggca cgccattttc gaataacaaa tacagagtta caggctggaa gctatgtcaa
     1741 acaaaccctt tatctaccag gcacctttcc cgatggggaa agacaatacc gaatactatc
     1801 tactcacttc cgattacgtt agcgttgccg acttcgacgg cgaaaccatc ctgaaagtgg
     1861 aaccagaagc cctgaccctg ctggcgcagc aagcctttca cgacgcttct tttatgctcc
     1921 gcccggcaca ccagaaacag gttgcggcta ttcttcacga tccagaagcc agcgaaaacg
     1981 acaagtacgt ggcgctgcaa ttcttaagaa actccgaaat cgccgccaaa ggcgtgctgc
     2041 cgacctgcca ggataccggc accgcgatca tcgtcggtaa aaaaggccag cgcgtgtgga
     2101 ccggcggcgg tgatgaagaa gcgctgtcga aaggcgtcta taacacctat atcgaagata
     2161 acctgcgcta ttcacagaat gcggcgctgg acatgtacaa agaggtcaac accggcacta
     2221 acctgcctgc gcaaatcgac ctgtacgcgg tagatggcga tgagtacaaa ttcctttgcg
     2281 ttgcgaaagg cggcggctct gccaacaaaa cgtatctcta ccaggaaacc aaagccctgc
     2341 tgactcccgg caaactgaaa aacttcctcg tcgagaaaat gcgtaccctc ggtactgcag
     2401 cctgcccgcc gtaccatatc gcgtttgtga ttggcggtac gtctgcggaa accaacctga
     2461 aaaccgtcaa gttagcaagc gctcactatt acgatgaact gccgacggaa gggaacgaac
     2521 atggtcaggc gttccgcgat gtccagctgg aacaggaact gctggaagag gcccagaaac
     2581 tcggtcttgg cgcgcagttt ggcggtaaat acttcgcgca cgacattcgc gttatccgtc
     2641 tgccacgtca cggcgcatcc tgcccggtcg gcatgggcgt ctcctgctcc gctgaccgta
     2701 acattaaagc gaaaatcaac cgcgaaggta tctggatcga aaaactggaa cacaacccag
     2761 gccagtacat tccacaagaa ctgcgccagg ccggtgaagg cgaagcggtg aaagttgacc
     2821 ttaaccgccc gatgaaagag atcctcgccc agctttcgca atacccggta tccactcgtt
     2881 tgtcgctcac cggcaccatt atcgtgggcc gagatattgc acacgccaag ctgaaagagc
     2941 tgattgacgc cggtaaagaa cttccgcagt acatcaaaga tcacccgatc tactacgcgg
     3001 gtccggcgaa aacccctgcc ggttatccat caggttcact tggcccaacc accgcaggcc
     3061 gtatggactc ctacgtggat ctgctgcaat cccacggcgg cagcatgatc atgctggcga
     3121 aaggtaaccg cagtcagcag gttaccgacg cgtgtcataa acacggcggc ttctacctcg
     3181 gtagcatcgg cggtccggcg gcggtactgg cgcagcagag catcaagcat ctggaatgcg
     3241 tcgcttatcc ggagctgggt atggaagcta tctggaaaat cgaagtagaa gatttcccgg
     3301 cgtttatcct ggtcgatgac aaaggtaacg acttcttcca gcagatcgtc aacaaacagt
     3361 gcgcgaactg cactaagtaa cctcttcggc ccagcgcctg gcagcatgct gccaggtgat
     3421 ccccctgggc cacctctttt gcgattgtaa tttcacgctt gctggtgaat agtcagtatt
     3481 ttccctgact tgcgaactca ccatgacccg cacactcaag ccgttaattc ttaacaccgg
     3541 cgcactggcg ctaacgttaa tcctgattta taccggcatt tcggcccatg acaaactcac
     3601 ctggctgatg gaagtgacac cggtgattat tgtcgtgccg ctactgcttg ccaccgctaa
     3661 acgttatccg ttaacgccgc tgctctatac gctcattttc tttcacgcca tcatcctgat
     3721 ggtcggcggg caatacacct acgcgaaagt ccccatcggt tttgaggtgc aggaatggct
     3781 ggggttgagc cgtaatccgt atgacaagct ggggcatttt ttccaggggc tggtgcctgc
     3841 actggtggca cgagaaattc tcgtgcgcgg gatgtacgtg cgcggacgta aaatggtggc
     3901 gttcctggtc tgctgcgtgg cgctggcgat aagcgcgatg tatgaattaa tcgagtggtg
     3961 ggcggcgctg gcaatgggcc agggagcgga tgattttctc ggcacccagg gcgaccagtg
     4021 ggacacgcaa tccgatatgt tctgcgcgct gcttggcgca ttaacgacgg tgatattcct
     4081 cgctcgcttt cattgccgcc agctacggcg ctttggcttg ataaccggat aacaacgtgc
     4141 gctttgttga tgccggatgc ggcgtgaatt ccttatccgg tctacaaaat ttgcaaatcc
     4201 aaagcattac attaagacgt ggcctgataa acatatcgca tcaggccatt ttcgttagct
     4261 attgtccacg tcaggctttc acatcactca ctgcaccgac cggaatatca gcatgaacag
     4321 gctcgggcgg tactttgcga tatttatcca gcagatgaat ctggatcctg cgcagcgtgt
     4381 cgccattgag gcgatagaaa cggaagtaga gaatcagcgt taccatgaaa aacagagttg
     4441 gtagagcaat cataataaac tgcataccta atagggcttg cgtagactgt tcaacattcg
     4501 gtacatagcc aatcatccct aacacaaccg caatgaaaaa agccgcaaag gctgaaccgc
     4561 ccttcaccac catagtctgc acggagtaag cgatactttc acagcgtacg tgcagtttat
     4621 attcaccgta atcaacggta tctgccacca tgatgacctg taatacccag aaaagcgccg
     4681 ttcccacatt cagcaaaata cccgcaatca caatgaggac gacgttgtga tagctcatta
     4741 atgccattaa caggagaaca ccacagctta acaccggaag aatagatgct ccggcccata
     4801 aaatgcgtcg ggataatgat ttaactaagc gtgggaagaa tactaacgtc accaggttag
     4861 cagctcccgc atacgacaga taatagggga acaaatccgc atcaccgata acatatgaga
     4921 aataatagat agcaaatccg gtaataatgt tgctggctac attataagca agagccatac
     4981 ccaagaggca tgaaagctga tcgtttttat aaattagcgc aacgatggct ttaagtgtca
     5041 gatggcttcc ttcagcagac ggttgattgt ctgacgaaaa gacttcatgc acattgcgca
     5101 gagtgatgat ggttgaaaca ataaaaaagg cgattagtac cagagtgaac atctgaaagc
     5161 caaatccccg atcgccaccg ccgacataat taacaaatgg tagcgtcaca cctgccgtaa
     5221 caaagcctgc cagactggca aaaaaacgcg gataaggaac cagttgttcg cgctcacgtt
     5281 tatcgagcgt gatggttgga accagcgacc agaagggaat atccataatg gtgtaagtca
     5341 tgccccagag gatgtaggtc acgcaaacaa agacaatctg agtagtacct tcaaacagat
     5401 gcgcactaaa gaggagaaat aagattacag agtttgccaa cgtaccgatc aggatccagg
     5461 gtttgaactt accccatcgc gatcgcgtag catttacaat ccatcccata atcggatcgt
     5521 taatagcatc ccagatcctc gccaccagaa acaaagtacc gaccaaaccc acagacagcc
     5581 caacgacatc ggtgtagtaa tacatgaggt acatatacac aatgccgatc gcaaaatcct
     5641 tcccgaacgc tccaaatcca taactgagtt ttgtagtcat tgaaatgctc atagggtatc
     5701 gggtcgctgt taccaacgcc cgccttctgt tattggtatc gtcagaggtg tgcgccggaa
     5761 taaatccccc ggcgcagtag cgtttagtcg cgttttaacg gtgcaaccag cctggcagcc
     5821 agtcgccgtg ggcggcaatc aggtcgtcaa caagagcata tatttcatca atgcccagca
     5881 cggcagcagt atgcgggtcc atcatcgcgg cgtggtaaac acggtcgcga ttttccgtaa
     5941 gaatagcttc ggtcagcagc gtctgtacgt tgatgttggt ttgcatcagg gcggccagat
     6001 gcgaaggtag cgtaccgact ttggtcggct gaatgccatt agcatcaacc agacaggcta
     6061 cttccacgca acatccttgt ggcaggttat caatcaaacc atcgttacgg acgttgccgt
     6121 aaatcacact cggctcgcca gtccagatag cgttcatgat tgtgctggca tattcccgtg
     6181 acggtttaat atcaatccgg gaggcgtttt tatactcctc cagctcttta tgccagttcg
     6241 ccagctgctc gacgcagcgt ttcgggtact catccagcgg tactttataa cgctcaatca
     6301 aatcctcacg acctggctta ataaaccacg gtgtgtactc agcaaaatgt tctgacgatt
     6361 ccgtgacgaa gtagcccagc tttttgaaca tttcgtagcg cacaatattc tggcagcgag
     6421 tattgccatg aatattcggc ttcggtgcct gccctgcttc ataagccgcc agcagttccg
     6481 ggtagagatt cacataactg ccgtcggcgg ttttgcgctc cagctccagg taaaacgcca
     6541 tatggttgat acctgcgcaa cggtaacgca gcgtagctgg gtcgatattg aggtcacgcg
     6601 ccagctcttc cgccgttccc tgcaccgaat ggcacagccc gacctgtttg atatgcggat
     6661 agcgggcata catcgcccag gtattcatcg ccattgggtt aacatagttg agcatggtgg
     6721 catcggggca gacttccgtc atgtcctcgc aaatttgcca cagatgcgga atggtacgta
     6781 gcgcgcgcat aataccgccc ggccccaacg tatcggcaat ggtttgttcc agaccatgcc
     6841 gcttacagac ctcgaaatca gtcaccgtgc aaggttcata accgccaatc tgaaatgcca
     6901 ccacgacaaa atcggcatcc tgtaaggctt ctttctgttg ggtgtggcag gtgattttgc
     6961 cgctggcccc tgctgaatcc atcagcttac gcaccacaat atgcgactct tccaggcggg
     7021 tgggatcaat gtccatcagg gcaatatgcg ccgttttcag cgcctcgcga tggaacacat
     7081 caccaagaat atttttaacg aaaatcgtcg aaccagcgcc gataaatgta attttgggtg
     7141 cagtcatcat gcagatctcc tggcttgctt gaataacttc atcatggcag gcgaatctgc
     7201 agaaaacctc cggcttccag gttgagcatt cccataaact cagatcctgc gtgaagcagc
     7261 agtaaatctg agtttatggt aataagttac ctgaaaagca gagttttcgg cctcatgatg
     7321 ctcggacgct cccctcgcct ctctgggaag agggtgaaaa ccgtgctccc actcgcagtc
     7381 atcctccctc actcctgcca taattctgat attccaggaa agagagccat ccatgaatac
     7441 agatacgttt atgtgcagca gcgacgaaaa acagacccgc agcccgctgt cgctgtattc
     7501 ggaataccag cggatggaga ttgagtttcg cgcaccgcat atcatgccca ccagccactg
     7561 gcatggtcag gtcgaagtga atgtgccttt cgatggcgat gtggaatacc tgatcaacaa
     7621 tgaaaaagtg aatatcaatc agggtcatat cacgctgttc tgggcctgta caccgcacca
     7681 actaacagat accggaacct gtcagagcat ggcgattttt aatctgccga tgcatctgtt
     7741 tctctcctgg ccgctggata aagacctgat taaccacgtc actcacggca tggtgatcaa
     7801 atcactggcc acacagcaac ttagcccgtt tgaagtgcgc cgctggcagc aggaattaaa
     7861 cagtccgaac gagcaaattc gccagctcgc cattgatgaa attggcctga tgctcaagcg
     7921 atttagcctc tctggctggg aaccgattct ggtcaataaa acctcgcgca cacacaaaaa
     7981 cagcgtctcg cgccatgcgc aattttatgt tagccagatg ctgggcttta ttgccgaaaa
     8041 ctatgatcag gcgctgacca tcaacgatgt ggctgagcac gtcaaactta acgccaacta
     8101 tgcaatgggg atattccagc gggtcatgca attgacgatg aaacagtaca ttaccgcgat
     8161 gcgcatcaac cacgttcgcg cgttactgag cgataccgat aaaagtattc tcgatattgc
     8221 cctgacggca ggctttcgtt caagtagtcg tttttacagc acgttcggca aatatgtcgg
     8281 catgtcgccg caacaatacc gcaaacttag ccaacagcgc cgccagacgt ttcccggcta
     8341 aagacgatat cagtatcagc caaaaaaata gtttagccgt cgataataag cagtgaaaat
     8401 aatttaaaat aatcacataa cgtattgttt ataaaattaa atatttcaaa aatgtttgtt
     8461 tttcacgcgc tttacagccc gaaaaggccg gaagatactt gcccgcaacg aagattcctt
     8521 cataaccggg taagcaatga tgaaagtatt aattgttgaa agcgagtttc tccatcaaga
     8581 cacctgggtc ggtaacgccg ttgagcgtct ggcagatgct ttaagccagc aaaatgttac
     8641 cgtgattaaa tccacctcct ttgatgatgg ttttgccatt ctctcttcaa acgaagccat
     8701 tgactgcctg atgttcagct atcaaatgga acatccggac gaacatcaaa acgtcagaca
     8761 attgatcggt aagcttcatg agcgccaaca aaacgtgccg gtcttcctgt tgggcgatcg
     8821 ggaaaaagcc ctcgccgcaa tggaccggga tctgctggag cttgtcgatg aattcgcctg
     8881 gattctggaa gataccgccg actttatcgc cggacgcgcc gttgccgcga tgacccgcta
     8941 ccgccagcag ctgttgccgc cactgttcag cgcgctgatg aaatatagtg acatccatga
     9001 atattcctgg gcagcgccag gccaccaggg cggcgttggt tttaccaaaa cacccgccgg
     9061 acgtttctac catgactact atggtgaaaa tctgttccgc accgacatgg gcatcgaacg
     9121 aacttccctc ggttctttgc ttgaccatac tggcgcattt ggcgaaagcg aaaaatatgc
     9181 cgcacgcgta tttggtgccg atcgctcctg gtcggtagtc gtcggtactt ccggctctaa
     9241 ccgcaccatc atgcaggctt gcatgaccga taacgatgtc gtggtcgttg accgtaactg
     9301 ccataaatcc atcgaacaag gtttgatgct gacaggcgcg aaaccggtct atatggtgcc
     9361 aagccgcaac cgctacggca ttatcgggcc aatctatccg caggaaatgc aacctgaaac
     9421 cttgcagaag aaaatcagtg aaagcccgct gaccaaagac aaagccgggc aaaaaccgtc
     9481 ttactgcgtg gtgaccaact gcacctatga cggcgtgtgt tataacgcta aagaagcgca
     9541 ggatctgctg gaaaaaacct ccgatcgtct gcactttgac gaagcctggt acggctatgc
     9601 acgtttcaac ccgatctatg ccgatcacta tgccatgcgc ggcgaaccag gcgatcacaa
     9661 cggtcctacc gttttcgcca cccactccac ccacaaactg ctgaatgcgc tgtcacaggc
     9721 ttcttatatt catgtacgtg aaggtcgtgg ggcgattaac ttctcccgct tcaaccaggc
     9781 ctacatgatg catgccacca cctccccgct gtatgccatc tgcgcatcca acgacgtggc
     9841 ggtgtcgatg atggacggca acagcggcct gtcactgaca caggaagtga ttgacgaagc
     9901 ggttgatttc cgtcaggcga tggcgcggct atataaagag ttcaccgctg acggtagctg
     9961 gttcttcaaa ccgtggaaca aagaagtcgt caccgaccca caaaccggca aaacctatga
    10021 ctttgctgac gcaccaacca aactgctgac caccgttcag gactgctggg taatgcatcc
    10081 gggcgaaagc tggcacggct tcaaagatat tccggataac tggagtatgc tcgacccaat
    10141 taaagtcagc atccttgctc cgggaatggg tgaagatggt gaactggaag aaaccggtgt
    10201 tccggcggcg ctggtcactg cctggcttgg tcgccacggc attgtgccta cccgcaccac
    10261 tgacttccaa attatgttcc tgttctctat gggcgtaacc cgtgggaaat gggggactct
    10321 ggttaacacc ctttgctcct tcaaacgcca ctatgacgcc aacacaccgc tggcgcaggt
    10381 gatgccggaa cttgttgaac aatatcctga cacttacgcg aacatgggga ttcacgatct
    10441 gggtgacacc atgtttgcct ggctgaaaga aaacaaccct ggcgcacggt tgaacgaagc
    10501 ctattccggc ctgccgatgg cggaaatcac cccgcgtgaa gcgtacaacg cgattgtcga
    10561 caacaatgtc gaactggtat ccattgaaaa tctgccagga cgcatcgcgg caaactcagt
    10621 tatcccgtat ccgccaggaa tcccgatgct gctgtctggt gaaaacttcg gcgataaaaa
    10681 cagtccgcaa gtaagttatt tacgctcgct gcaatcctgg gaccaccatt tccctggatt
    10741 tgaacacgaa actgaaggga ctgaaattat tgacggtatt taccacgtta tgtgcgtgaa
    10801 agcgtaacca ctattccgct gaaggcgtaa ttgtttaaat aacattacgc cgcctggcct
    10861 taggcctttt gagtatggca acgttttcat aaaaattgct gcaaacaaaa atgtcatact
    10921 ttttgcgcgg ccccaccccg
//
LOCUS       DAZKFU020000153        10926 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZKFU020000153 DAZKFU020000000
VERSION     DAZKFU020000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.154"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..179
                     /locus_tag="ACHOQQ_RS16340"
                     /old_locus_tag="pgaptmp_003268"
     CDS             <1..179
                     /locus_tag="ACHOQQ_RS16340"
                     /old_locus_tag="pgaptmp_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471480.1"
                     /translation="YEFARMIFAYINDKSIEILLLSCFAAADQKAIQRYIPQAQDGED
                     FRNHLQYKLTLPTH"
     gene            390..713
                     /gene="ybjQ"
                     /locus_tag="ACHOQQ_RS16345"
                     /old_locus_tag="pgaptmp_003269"
     CDS             390..713
                     /gene="ybjQ"
                     /locus_tag="ACHOQQ_RS16345"
                     /old_locus_tag="pgaptmp_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGU4471481.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            710..1540
                     /gene="amiD"
                     /locus_tag="ACHOQQ_RS16350"
                     /old_locus_tag="pgaptmp_003270"
     CDS             710..1540
                     /gene="amiD"
                     /locus_tag="ACHOQQ_RS16350"
                     /old_locus_tag="pgaptmp_003270"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGU4471482.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     STWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMRPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1537..2550)
                     /gene="ybjS"
                     /locus_tag="ACHOQQ_RS16355"
                     /old_locus_tag="pgaptmp_003271"
     CDS             complement(1537..2550)
                     /gene="ybjS"
                     /locus_tag="ACHOQQ_RS16355"
                     /old_locus_tag="pgaptmp_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4471483.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2649..4079)
                     /gene="ybjT"
                     /locus_tag="ACHOQQ_RS16360"
                     /old_locus_tag="pgaptmp_003272"
     CDS             complement(2649..4079)
                     /gene="ybjT"
                     /locus_tag="ACHOQQ_RS16360"
                     /old_locus_tag="pgaptmp_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGU4471484.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGESGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLSLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4090..5091)
                     /gene="ltaE"
                     /locus_tag="ACHOQQ_RS16365"
                     /old_locus_tag="pgaptmp_003273"
     CDS             complement(4090..5091)
                     /gene="ltaE"
                     /locus_tag="ACHOQQ_RS16365"
                     /old_locus_tag="pgaptmp_003273"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGU4471485.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLNKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5128..6846)
                     /gene="poxB"
                     /locus_tag="ACHOQQ_RS16370"
                     /old_locus_tag="pgaptmp_003274"
     CDS             complement(5128..6846)
                     /gene="poxB"
                     /locus_tag="ACHOQQ_RS16370"
                     /old_locus_tag="pgaptmp_003274"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGU4471486.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(6979..7947)
                     /gene="hcr"
                     /locus_tag="ACHOQQ_RS16375"
                     /old_locus_tag="pgaptmp_003275"
     CDS             complement(6979..7947)
                     /gene="hcr"
                     /locus_tag="ACHOQQ_RS16375"
                     /old_locus_tag="pgaptmp_003275"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGU4471487.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFITGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(7959..9611)
                     /gene="hcp"
                     /locus_tag="ACHOQQ_RS16380"
                     /old_locus_tag="pgaptmp_003276"
                     /gene_synonym="priS"
     CDS             complement(7959..9611)
                     /gene="hcp"
                     /locus_tag="ACHOQQ_RS16380"
                     /old_locus_tag="pgaptmp_003276"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGU4471488.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9755..10654)
                     /gene="lysO"
                     /locus_tag="ACHOQQ_RS16385"
                     /old_locus_tag="pgaptmp_003277"
     CDS             complement(9755..10654)
                     /gene="lysO"
                     /locus_tag="ACHOQQ_RS16385"
                     /old_locus_tag="pgaptmp_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGU4471489.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2642 a         2903 c         2906 g         2475 t
ORIGIN      
        1 tatacgaatt tgcaagaatg atcttcgctt atattaatga caaaagcatt gagatcttat
       61 tactttcttg ttttgcggcg gcggaccaaa aagcaatcca gcggtatata cctcaggcgc
      121 aagatgggga agactttagg aatcatttac aatataagtt gactcttccc actcattaat
      181 gttgccggca caacataata gggcaggtaa gcggtgctcc ataatgaaac caatatccgg
      241 ttacgaaaca gataattgta caggcttatg tagctgtttt tgacacaatg agatgatttc
      301 ctaaactgat caaaaccttc tttccaacat ggcatatcag tccgataatg ctttagagat
      361 cgaaggctta acaaacaagg agatcgatca tgcaattttc cacaactcca actctggaag
      421 gccagaccat cgttgaatat tgcggtgtgg tgaccggcga agcgatttta ggtgccaata
      481 ttttccgtga tttctttgcc ggtatccgcg atatcgttgg cggacgttcc ggtgcgtatg
      541 aaaaagaact gcgtaaagca cgggagatcg cctttgagga attaggctcc caggcgcggg
      601 cgctgggggc cgatgccgta gttggtattg atatcgacta cgaaacggtc gggcaaaacg
      661 gcagtatgct gatggttagc gtcagcggta cggcggtgaa aacgcgtcga tgagaagagt
      721 cttctggctg gtcgctgccg ctctgttact ggcagggtgt gcaggcgaaa aaggcattgt
      781 cgagaaagag ggataccagc ttgatacccg acgccaggcg caggcggcgt atccgcgcat
      841 taaagtgctg gtgatccact acaccgcaga tgattttgat agctcgctgg cgacactgac
      901 cgataagcag gtcagctcgc attatctggt ccctgcggta ccaccgcgat acaacggtaa
      961 accgcgcatc tggcaactgg tgccggaaca agaactggcc tggcatgcgg ggattagcac
     1021 ctggcgcggg gcaacgcgcc ttaacgacac ctctattggc attgagctgg aaaaccgtgg
     1081 ctggcaaaaa tcggccggag tgaaatattt tgccccgttt gaaccggcac agattcaggc
     1141 gcttattcca ctggcgaaag atattattgc ccgttatcac atcaagccgg aaaacgtagt
     1201 ggcacatgcg gatatcgcac cgcagcgcaa agacgatccg gggccattat ttccctggca
     1261 gcaactggcg cagcagggga ttggtgcctg gccggatgcg cagcgggtga acttttacct
     1321 tgccgggcgc gcgccgcaca caccggtaga taccgcgtca ttgctggagc ttttggcgcg
     1381 ctacggttat gacgttaaac ctgatatgag accgcgcgag cagcggcgcg tgattatggc
     1441 gttccagatg catttccgcc cgacgttata caacggcgaa gcggatgcag aaactcaggc
     1501 aattgccgaa gcgttgctgg agaaatacgg gcaggattag cgcggcagtt ttccgtggtc
     1561 gcgtagccag gcggcagttt tctcgatacc ttcatccagg gtgatgaccg gctgataacc
     1621 taattcttcc tgtgcacgcg taatatccag cgtaaagtca aaattaagct tcgagacgcc
     1681 gtagtgggtc agcggcggct cttttgctga cttgcggcct aaacgctcca tgctgcgggc
     1741 gatcatatcc agcatcgggt aggggacgga acgaatacga cagtcaatat tcaactcgtc
     1801 gatcagcttc tgcacgatgc tgcgcagtgt gcgatgctcg ccgttggtaa tgttgtacac
     1861 acgcccggaa ggtagcttat cgcaggcttc ctggcttgcc agccacatgg cgtgcactgc
     1921 attttcatag taggtcatat cgaccagcgc acttccgccg tgcggtaaca gaatgctgcc
     1981 gtagtggtgc atcatatgcg caagacgggg gataaagact ttatcgtgcg gtccgaacag
     2041 actttgtggg cgcagaatag taaagcgcgt ttgtggattc gcctgcgaaa gcatattgat
     2101 cacttcttcg ctggctgctt tgctgcgggc aaactcgttg gcgaagcggt gagggcgaaa
     2161 atcttcttta atatcgcgat ggtggtgata atcgaagtac agggagggag aagagatatg
     2221 aataaagtta cgcacacccc aggcgacagc ccattcaccc aggcggcgag tggcgcgaac
     2281 gttagccaga tcgaaagcct gttgtgtccc ccagggagag gtaaagctgg agcagtgcca
     2341 cagcgtatca atgcccgcga gcatcacttt agcttgtgat gagaccagct cggtcagatc
     2401 cgccggaaca aactctgcgc ccattttttc cagcaattta cccattgcct cgttgcgacc
     2461 ggtcgctcgc acgctgatgc ctttctggca taaaaactct accgcgtttc gacctaagcc
     2521 gctggtggcg ccggtaacca gtaccttcat atcaatccac tgttgttgag aaaatgacgt
     2581 gcgcattctt ccgtgatttc ccccatgatg caatgggaaa catgaaagaa taacgcaggt
     2641 tttgtcgatt aatctgtgct ttgttctgcc agtctggcga tttgttttgc cattccgcga
     2701 aaaataaaca gatgcgcggg gatcatcaat aaccagtaaa acagccccgg cataccgtgc
     2761 ggatgccaga aagcgcggac atcgatagta cgatagtcgc ctttatcttc cagggtaaaa
     2821 cacagtcgtc ccagccccgg tgctttcatg ccaaataaca acgaaagttg ctttttcggt
     2881 tcaacgacaa tgactttcca gctatccacc gcatcgccag tttgcaaata ttcgcgctcc
     2941 ggacggcctt tcgctaattt atgaccgatc gcacggtcca tcaacgcccg tgtctgccac
     3001 aaaatattgc caaagaaata acgctcttta ccgccgattt ggttcactac ctgccataaa
     3061 gccgcaaggc tggcggacgt tttaacggta aaccccgcct gtttggcgaa ataaccgtac
     3121 tcaggtcgcc agcgggcaaa ggcctgagcg tcgtagcccc agtcgctgga gttgaccagt
     3181 ttttcttcct ctttcaacgt gctacgtacc gcgtcatcga aagcgatcag ccgttgtggg
     3241 atgagtgcac gtagcgcggt gtcatccgcc agcagatcgt gtttcagccc ctgaatcaac
     3301 gccctggcgg tggtgggcgg tacggaagta atcacattaa gaaaccacac cgaaatccag
     3361 cgagtgggga gggggatggg gatcaaccag cggcgcttac cgctcaccgc cataaaatgt
     3421 tcaaactgtt gctgataact gagcacctct ggtccggcgg cttcgaagat gcggtgttcg
     3481 ctggccggat gatctaacag cgccaccaga tagtgcagca agttttccag cgcgatgggc
     3541 gtggtgcgtg aacgaaccca gcgtggcggc gttaacactg gcagattgta gaccatatcg
     3601 cgcatgactt cgaacgctgc tgaacctgcg ccaacgataa ttccggcccg cagttcggtc
     3661 accgggacac ctgcttcacg aagaatatcc gccgtagcct gacgagcacg cagatgatcc
     3721 gactgctcat gtggcggggc ctgtaacgaa ctgagaaaga ttaattgctt aactggtact
     3781 tcacgtagcg catcgcggac gttgagagcc acctggcgct cctgagcgat aaaatcgccg
     3841 ctttcgccca tgctgtgcac cagaaaatag acggtatcga tatcctgtaa cagggccgga
     3901 aggttatccg gccagctgag atcgacttta tggcaactga cgtttgccag ttgcagcttt
     3961 gcaagcctgt cgacatgacg tgccgccgcc aggatctgat gcccttgctg gctgagtgtg
     4021 cgcaccagat gctgaccaat gtagccactg gcaccgagaa ctaaaatgcg ttgcggcacg
     4081 tttctctcct taacgcgcca ggaatgcgcg ccagtgggcg gcgacttccg ccagctgttc
     4141 gcgcgagacg tcaagatgcg tcaccaggcg gacaatcggc gaggcgttaa tcagcacgtt
     4201 tctcgctttc atgtattcgc ctaacgcggc agcattttct tccccgacgc gaacaaacag
     4261 catattagtg tcctgacgca tcacatccgc gcctgcttca cgcagctgct ccgccatcca
     4321 ggcagcgttg tcgtggtctt cctgcaagcg cgcaacatta tttttcaggg catatatccc
     4381 ggcggcagcc agaatgccgg actggcgcat cccgccacct gtcattttcc gccagcgaat
     4441 ggcacgttta atgtaatcac gattaccgac gagtaatgaa ccgactggcg tcccaagacc
     4501 tttcgacagg caaatggtga acgaatcaca atattgcgtg atctctttca gttcgcagcc
     4561 gtaagccacc acggcattaa agatgcgcgc accgtcaaca tgcagcgcca gattgcgctc
     4621 gcgggtaaat tcccatgcat ctttaaggta ttcacgcggc aacactttgc cgttgtgggt
     4681 gttttccaga ctgagtaatt tggtgcgggc gaaatggata tcgtcgggtt tgattttcat
     4741 cgccacttta ttcagcggta gcgtgccgtc ggcagccgcg tcgatgggtt gcggctgaat
     4801 actgcccagc accgctgcgc caccggcttc aaacagatag ttatgcgcgg cctgaccgac
     4861 aatatactct tcgccgcgtt cgcagtgact gagcagagcg accaggttgg cctgagtgcc
     4921 ggtaggcaga aaaatggcgg cttctttacc ggaaagctct gctgcatagt cctgcagagc
     4981 attaacggta gggtcgtctc cgtaaacgtc gtccccaacc ggggcggcca tcatcgcttc
     5041 gagcatggcg cggctcggtc gggttacggt atcactgcgt aaatcaatca tggcatgtcc
     5101 ttattatgac gggaaatgcc acccttttta ccttagccag ttcgttttcg ccagttcgat
     5161 cacttcatcg ccgcgtccgc tgatgattgc gcgcagcata tacaggctga aacctttggc
     5221 ctgttcgagt ttgatctgcg gtggaatggc taactcttct ttggcgacca ccacatccac
     5281 caataccgga ccgtcgatgg agaaggcgcg ttgcagggct tcatcaactt cagacgcttt
     5341 ttctacacgg atacccgtaa tgccgcatgc ttcggcaatg cgggcaaagt tggtgtcgtg
     5401 cagctcagta ccgtctgtca ggtagcctcc ggctttcatc tccatcgcca caaagcccag
     5461 cacgctgtta ttaaagacga taattttcac cggcagtttc atctgcacta ccgagaggaa
     5521 atcgcccatc aacatgctaa aaccgccatc gccgcacatg gcgaccacct gacgttccgg
     5581 ctcggttgcc tgcgcgccca gcgcctgcgg catggcgtta gccatcgaac cgtggttaaa
     5641 cgaacctaac aggcgacgct tgccgttcat tttcagataa cgcgctgccc acaccgttgg
     5701 cgtaccaacg tcacaggtga aaatggcgtc atcggcggca aaatgactaa tttgctgcgc
     5761 cagatattgc gggtgaatgg ctttctcgct cggtttagct aaatcgtcca gccctttgcg
     5821 ggcatcgcgg taatcttcca gcgctttatc cagaaatttg cgatcggctt tttcttccac
     5881 cagtggcagc aatgcacgca gggttgattt gatatcgccg accagtgcca tatccacctt
     5941 gctgtgagcg ccgatgctgg ctggattgat atcaatctga ataattttgg catcggtcgg
     6001 gtagaacgca cggtagggga actgagtacc gagtagcact aacgtgtcgg cgttcatcat
     6061 ggtatggaaa cctgacgaga atccgattaa cccggtcatt ccaacatcat acggattatc
     6121 gtattcgaca tgctctttac cgcgtagggc atgaacaata ggtgctttaa ttttcccggc
     6181 aaactcaact aactctttat gcgcccccgc gcagccgctg ccacacatca gagcgatatt
     6241 gctggaataa cgcagcagtt gcgccagttt gcgtaactct tcttcttccg gcgtcacaac
     6301 tggctgtggc gcatgatacc agtgcgtggt tgccccttct ggcgcaggtt ttaacgccac
     6361 gtcacccggt aacacgacaa cggaaacgcc acggttaagc accgctttgc gcatagcaat
     6421 tgccagcact tgtgggatct gctccgggct ggaaaccagc tcgcaatagt gactacattc
     6481 gcggaatagc tcttgtgggt gggtttcctg gaaatagccg ctgcctattt cgctggaggg
     6541 aatatgagcg gcaatcgcca gtaccggaac gtgattgcgg tggcaatcga acaggccgtt
     6601 gattaagtgc aggttgccgg ggccgcacga tccggcacag accgccagtt ctcctgaaag
     6661 ttgtgcttca gcgccagcgg caaacgccgc cacttcttca tggcgggtgg acatccactc
     6721 gatggtgccc atgcgattaa gactgtcgct aagaccgttc agagagtcgc ccgtgactcc
     6781 ccagatgcgt ttcacgcctg ccgattcgag tgttttggcg atataagctg caaccgtttg
     6841 tttcatggtt ctccatctcc tgaatgtgat aacggtaaca agtttagttc atctgacgga
     6901 gggggaaggg atgggagaaa aaggaggcac taacggttaa atagcccgat gaaatgaata
     6961 tcatcgggca taaggcgatt atgcgagaac caaatccccc tgcggatggc aggagcaggc
     7021 cagtacgtaa ccttcagcga tttcggcgtc ggtcagcgtc attgtgctgc tcaccgtata
     7081 ttcaccggaa accacttttg tcttacagca gccgcaaaca cccgcacggc aggcagcgac
     7141 aaccggaacg ttattgcttt ccagcgcctc cagtagcgta gtgccaaccg gggcgtaaaa
     7201 ttctcgtgcc ggttgcagtt tggtgaattt cagaccgctg gtcgccgctt ccgctactgg
     7261 ggtgaagaat ttctctttaa agaaacgcgt cacgccgagc gctttcactt cctgctctac
     7321 ccaatccata tacggtgctg gaccacaagt catcacggta cgtgaagcta agtcaggaac
     7381 gcgagtcagg agttcgcgag tgagacgacc agtgataaag ccttcggtaa cgttattttc
     7441 cgccaccagc gttaccggat agttacgcca ctcatcggcg aaaataacat cctgcggcgt
     7501 acgcacgttg tagatcacct gtacatcggc ctgtggacga ttcttcgcca gccagcgacg
     7561 catcgacata atcggcgtga cgccgcagcc tgcagccagc aacaggaatt tatcttctgc
     7621 tttatcgtcg caggtaaatt cccccatcgc gtccgaaagc cagagataat caccgcgttt
     7681 tacatcgcgg gtcagccact gggagccgac accgtcatca atccgccgca cggtcagggt
     7741 gatatattca ctcacgcctg gcgtggagga aatggtgtaa gcacgcagcg tttccgctga
     7801 gttacgcacg ctgaccagtg catattgccc ggcgcggtat gggtagtaat cgtggcaaat
     7861 cagggaaatc gtccacacat ccggcgtttc ttgcttaatg tgatgaacct gcatccgcca
     7921 cgggcattga ttcgttggca tcgtcatcga caaactcctt acgcgctcaa cagttgcttc
     7981 atgtcttctt caacagtggt gatagaacgc aggccgaatt tctcgttcag caccgccagc
     8041 agatctggtg tcaggaaacc aggtgcagtc gggccggtga cgatattttt cacgcccaga
     8101 gaaagcagcg tcagcagaat gacgatcgct ttctgttcaa accaggagag caccagcgac
     8161 agcggcagat cgttgacacc gcagcccagt ttctctgcca gcgtaaccgc cagaataatc
     8221 gctgagtaag catcgttaca ctgaccggca tctaccagac gcggcagacc ttcgatatcg
     8281 ccaaactcaa gtttgttaaa gcgatattta ccacaggcga gggtcaggat caggcagtca
     8341 tccggcacgc tggtggcgaa atcggtgaag tagtggcgct cgccgcgtgc gccgtcacag
     8401 ccaccaagca ggaagatatg acgcagtttt tcacggctca ccagatcaat cagcgtatcc
     8461 gctgcgccaa gcagcgtctg gcggccaaag cccacggtga tcaggtgcgg aatttcgctg
     8521 tacgggaagc ccgccatttg ttgcgcctgg gcaatgaccg cagagaaatc ttcgccatcc
     8581 agatgacgca cacccggcca gccaacgatg ctgcgggtcc agatacgatc gtcataagcg
     8641 cctacggttg ggtcgatgat gcagttcgag gtcatcacga tggggccagg gaaacgagcg
     8701 aactccactt gctgattctg ccagccgctg ccgtagttac cgaccagatg cttgaattta
     8761 cgcagctccg ggtagccatg cgcaggcagc atttcgccgt gggtgtagac attaacaccc
     8821 gtgccttcgg tctgttccag aaggttgtag agatctttga gatcgtgacc ggaaatcaga
     8881 atgcatttac ccgccgtcgc tttgacgttg acctgggttg gcgtcgggtg accgtattta
     8941 ccggtttcgc ctgcatccag aatgctcatc actttgaagt tcatctggcc gatttccatt
     9001 gaacactcaa gaagcgcgtt catatcggca ggccaggtcc ccagccacgc catgatttta
     9061 tggtactggg cataaatatc attgtcgtat tgaccgagaa cgtgtgcgtg ttccatatag
     9121 gccgccgcac ctttcaggcc atacaggcac agcagacgca ggccgagaat gttttcgcca
     9181 atcgccgctt tatctttgtt aggggtaaat tctgctgcct gacgttgcag ttcgccgaga
     9241 tcgtcgctca ccagttgcag gtcagccatc gggttatcga cgcgggcgtt ggcatctaca
     9301 gccaggcatt gtgctttcag cgcctcgcgc aaggcaatcg cttcacgagc gtagccgaca
     9361 atacgcggag aatcgaagtt aacgttggtc agggttgaga aaaatgcgcg tggcgcaaag
     9421 ctgtctacat cgtggttgat gatgccgtat tcacgcgctt ttaccgccca ggccgaaagc
     9481 ccttgcagcg ccgcgatgag taaatcctga aggtcagagg tttccgccgt tttaccacac
     9541 atcccctgcg cgtatgagca gccgtttcct gccggagtac ggatagtttg ttcacattgc
     9601 acacaaaaca tgatcacacc ttttaaagtt atatttaata tacatcttta aggttaagac
     9661 gcttaacgcg gggataaaag ggatttttca tgcaacttta agggagattg atttagcgca
     9721 attttggcgg cagggatcta ccgccagaga ggtattacgc agagaaaaag gcgatgagga
     9781 tcggcactaa caggctaaga ataaaaccgt gaacaattgc cgccgggacc atatccagcc
     9841 cgccagtacg ttgaagaacg ggcagggtga aatccatcga cgtcgcaccg cataagccca
     9901 gtgcggtaga gcggctgcgg cgaatcagcc cagggatcaa cataattgca atcagctcac
     9961 gggccagatc attaaaaaac gccgcgctcc cgattaccgg accaaaagat tcggtcaata
    10021 aaataccgga aagagaatac cagccgaaac cagaggccat tgccagcgcg gtattgatgg
    10081 gtagatcaag aataaaggcg ttaattaaac caccaattaa tgaactggca accaccacca
    10141 cggcgacaat cattccccgg cgattaagga caatctgctt taaggtcatg ccattattgc
    10201 gcaactgaat accgacgagg aaaagtagca gaattaaggt gtattcactg gcttcggtcg
    10261 cgtgttgtaa gaaagccagt ccactcaggc caatggcaaa accaatcact actacgccgc
    10321 acagttttag cgactcaagc gccatcgcaa tacgcgacgg gagtttttct tgctgatggt
    10381 ggttgcgcca cggcagaccg cgctccagcc acataagggc ggcaatatta cacagtaaaa
    10441 taacggtaat actgacggca gaataatgca gaatcgccaa caggttactg gcgaggttat
    10501 cgagaaacgc cagactgata cccataaaaa agagaataag gtaaaccatc cagcttagta
    10561 gctgattaat aacttttaac gcagcttgtt ggcgaagcgg aatgaggtaa cccacaatca
    10621 ggggaaccag aatgattaac aacccagaaa acatgaaaac ccagcccttg caaagaaaga
    10681 agtcgaaatg cgcgatgaca cactactgaa agcggaagga cgagtaaagt tgcaattaaa
    10741 aggaaatgtt atgcataagg atcggtaggg tattcatttt catttaaaga tattcttgct
    10801 ctttaattac aaaccgcacc ggtgttggtg gcgtcaaaat cgccgaggcg ttccctgaag
    10861 gccggggcag cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg
    10921 cccgac
//
LOCUS       DAZKFU020000154        10923 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZKFU020000154 DAZKFU020000000
VERSION     DAZKFU020000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.155"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..824
                     /gene="adiY"
                     /locus_tag="ACHOQQ_RS16390"
                     /old_locus_tag="pgaptmp_003278"
     CDS             63..824
                     /gene="adiY"
                     /locus_tag="ACHOQQ_RS16390"
                     /old_locus_tag="pgaptmp_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGU4471490.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            961..2298
                     /gene="adiC"
                     /locus_tag="ACHOQQ_RS16395"
                     /old_locus_tag="pgaptmp_003279"
     CDS             961..2298
                     /gene="adiC"
                     /locus_tag="ACHOQQ_RS16395"
                     /old_locus_tag="pgaptmp_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGU4471491.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2402..4045
                     /gene="eptA"
                     /locus_tag="ACHOQQ_RS16400"
                     /old_locus_tag="pgaptmp_003280"
     CDS             2402..4045
                     /gene="eptA"
                     /locus_tag="ACHOQQ_RS16400"
                     /old_locus_tag="pgaptmp_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGU4471492.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4042..4710
                     /gene="basR"
                     /locus_tag="ACHOQQ_RS16405"
                     /old_locus_tag="pgaptmp_003281"
     CDS             4042..4710
                     /gene="basR"
                     /locus_tag="ACHOQQ_RS16405"
                     /old_locus_tag="pgaptmp_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGU4471493.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHOQQ_RS16410"
                     /old_locus_tag="pgaptmp_003282"
     CDS             4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHOQQ_RS16410"
                     /old_locus_tag="pgaptmp_003282"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGU4471494.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHOQQ_RS16415"
                     /old_locus_tag="pgaptmp_003283"
     CDS             complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHOQQ_RS16415"
                     /old_locus_tag="pgaptmp_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGU4471495.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHOQQ_RS16420"
                     /old_locus_tag="pgaptmp_003284"
     CDS             complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHOQQ_RS16420"
                     /old_locus_tag="pgaptmp_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGU4471496.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHOQQ_RS16425"
                     /old_locus_tag="pgaptmp_003285"
     CDS             complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHOQQ_RS16425"
                     /old_locus_tag="pgaptmp_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HGU4471497.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHOQQ_RS16430"
                     /old_locus_tag="pgaptmp_003286"
     CDS             complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHOQQ_RS16430"
                     /old_locus_tag="pgaptmp_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGU4471498.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQRAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2731 a         2853 c         2691 g         2648 t
ORIGIN      
        1 tttcccggta tcgctttgct ttagcgagaa ctggtctttt atttgcactc aggagtacat
       61 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      121 taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      181 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      241 acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      301 ggcaacgtag cgctacgccc atactcaccc tgccatgcct gacgccagac gtctttcgcg
      361 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      421 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      481 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      541 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      601 agaaaaagtt gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      661 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      721 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc
      781 cgctgcatta tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga
      841 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      901 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
      961 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1021 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc tactggcggg
     1081 attgccattt atggatggtt ggtgacgatt atcggtgcac tggggctctc gatggtatac
     1141 gccaaaatgt cgttcctcga cccaagtcct ggtggttctt acgcttacgc ccgccgctgc
     1201 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1261 aatatcgcca tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1321 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1381 attgtcggtc cgaaaatgat cacccgtgtg caggcagttg ccaccgtgct ggcgctgatc
     1441 cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg
     1501 gcatggaacg tcagcggcct gggcaccttc ggtgcaattc aaagtaccct taacgttacg
     1561 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1621 aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgta
     1681 ctttctacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcttcg
     1741 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ccggggccat tgtttccttc
     1801 tgcgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg
     1861 gcgaaagccg ctgccgatga cggactgttc ccaccaattt ttgcccgtgt aaataaagcg
     1921 ggtacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagc
     1981 agcatttcac caaacgcgac caaagagttc ggtctggttt cttccgtctc ggtcatcttt
     2041 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggaca cggtcacttt
     2101 ggtaaagcac gcccggcata tctggcagtt actaccattg ccttcctcta ctgcatctgg
     2161 gccgtggtgg ggtccggagc gaaagaggtt atgtggtcat ttgtcaccct gatggtcatc
     2221 accgccatgt atgcgctgaa ttacaaccgg ctacataaaa acccgtatcc cttagatgca
     2281 ccaataagca aagattaatt ccccttaatc cagcaaacat aaaagccaac cttaagaact
     2341 taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt
     2401 gatgttgaag cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc
     2461 cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct
     2521 gccgctggat tcgctgcata acgtactggt tttcttgtcg atgccggtcg tcgctttcag
     2581 cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac cactggcctg
     2641 cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat
     2701 cgaccgctcg atgattgcca atattattga taccactccg gcagaaagtt atgcgctgat
     2761 gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgcgc tgattgcctg
     2821 ctggataaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa
     2881 tattctggtt tctgtactac tgattttgct ggtcgccgca ctgttttata aagactacgc
     2941 ctcgttgttc cgcaataaca aagagctggt gaaatcctta agcccctcta acagcattgt
     3001 tgccagctgg tcatggtact cccatcagcg actggcaaat ctaccgctgg tgcgaattgg
     3061 tgaagacgcc caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt tgaccatcct
     3121 gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac
     3181 taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac
     3241 ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc attacaaaga
     3301 agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt
     3361 gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa
     3421 cgtcaccgcg ctgaatctgc ctggtcagtg catcaacggc gaatgctatg acgaagtgct
     3481 gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca
     3541 caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt
     3601 taccccaacc tgcgacacca atgagatcca gacctgtagc aaagagcaac tggtgaacac
     3661 ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcgatta atctgctgaa
     3721 agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt
     3781 aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa
     3841 acaggtgcca atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca
     3901 gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac
     3961 gctattggga ttaactggcg ttgagacgaa gtattaccag gctgcggatg atattctgca
     4021 aacttgcagg agagtgagtg aatgaaaatt ctgattgttg aagacgatac gctgttattg
     4081 cagggattga ttctggcggc gcaaaccgaa ggctacgcgt gcgatggcgt gacaaccgcg
     4141 cggatggcgg aacaaagcct tgaggccggt cattacagcc tggtggtact ggatttaggg
     4201 ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa atacaccctg
     4261 ccagtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgccgg gctggatgtc
     4321 ggtgccgatg actatctggt gaagcctttt gcgctggaag agttacacgc ccgtatccgc
     4381 gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg
     4441 ctgaatatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa
     4501 gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtgca tcgggaaatt
     4561 ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat
     4621 atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc
     4681 tatatgctgg tcgcgaatga ggaaaactaa ttgaatctga tgcattttct gcgccgacca
     4741 atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg
     4801 atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg
     4861 ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc
     4921 agcctgattg tccccggcgt gtttatggtc agcctgacgc tatttatctg ctatcaggcg
     4981 gtacgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcaccgcc
     5041 gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg
     5101 gcgttgaacg atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttgtttacc
     5161 gctgacgtcg cgcacgaact gcgaacgcca ctggctgggg tgcgtttgca tctggaactg
     5221 ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacggcttga tcagatgatg
     5281 gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat
     5341 tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc
     5401 atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt
     5461 cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt
     5521 tacagcccgc aaggcagcaa cattatgatt aagctgcaag aagatggcgg agcggtcatg
     5581 gcagttgaag atgaaggacc aggtattgat gagagtaaat gcggggagtt gagtaaagcg
     5641 tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc
     5701 attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg
     5761 cgggcctggg tacggctgaa gaaagatcag aacgtggcaa accagatata aagaaagctg
     5821 ctgaccacca gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca
     5881 ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt
     5941 tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagctta ttcatcaatt
     6001 cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca
     6061 atattatcga ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc
     6121 tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg
     6181 gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc aggcatcatc
     6241 agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc
     6301 accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct
     6361 ggcaaggtag aagccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac
     6421 ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac
     6481 acaaacaggg caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc
     6541 aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc
     6601 ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac
     6661 atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag
     6721 tgtttggtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg
     6781 ccctgttcca gtttatcgac atgctgctgg aacgctgggg tctcttccag cgcatggcgc
     6841 aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag
     6901 tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccactacgcc agcacccagc
     6961 acaaacccgg caatagaacc aaagtccagc cagctgccca taaagccacg tttacggtca
     7021 ggggagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct
     7081 tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac
     7141 ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc
     7201 ttctggcgac catatttatc gcccaacata ccaaagaaga gtccgccaag cggtcgaatc
     7261 agaaagggaa cggagaacgt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc
     7321 gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcaaaccat
     7381 tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata
     7441 atggtgacat cacgaagggt aatcggtttt acttttttcc ttttcagcat agctttcctc
     7501 gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga
     7561 gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg
     7621 ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac
     7681 cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga
     7741 tgtaactgat tatttacgcc aaatttacag agaagatttc ctcgaccatt gcccccgcca
     7801 gacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac agtttgccag cgttgggtca
     7861 ctgaccagca aacgggttgt ttatctgccg tggtccactt tcccagccaa cttaaaaaat
     7921 cagcatgatc cccgcccgtc ggaacggtcg aagaggcaaa ccactgatgg taatagtgac
     7981 gcgtggtcgg ggccgcattg atactctctg ccagatgttg tgtcgctttc acgcgcaaat
     8041 cacccagcaa caacgccacc gcttgcgggc ttcccagacg acaggcatcg ccaaatgccc
     8101 cccaacgcaa ctcttcgaga acgataaaac agcgcccagg caacgaccag gcgttatacg
     8161 ccccagcacg ccagcgggta aaaatttgct cgctctgatc acctgcctgc accagtaggc
     8221 ctcgttgcgt taacgccttt tccttccagc ggcaacgggc tgaaaaccac tcatacaagg
     8281 cggcaatttg ctgggggagc tccgggaaag cagagtgctg gcggtgatcc attttttgca
     8341 gcagcgccag atgctgcgcg atctgcgtca gcgccagatg accgggatcc agcatcgacg
     8401 ccagtttttc caccattgtc agtcgcaggc taagcggcgc atttaacatg ccgccgttcg
     8461 tccatgtctg aagctgcacg cgcgcaagaa gttcctgcat cagtcgttca cgaaactgct
     8521 gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc cagatccact aaaaagcggg
     8581 gataaaccga ctccagcacg cgaccggggc cgtcaagtaa cgtcttggtc aatatcgttc
     8641 tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg gcgtcggtgt aaatccagcc
     8701 gtgagtcatc acagtctgct gcaattgctg ctgcatcagc ctgaccaccg attcattttg
     8761 ttgacgcaga gccagacttt cgcgtaaacg cgtctgtaat tccgtcaaac atgaagcgaa
     8821 ctcagcgaaa aaagtattca tgcctgccgt aacagattca tcgacctgct ctgccagaac
     8881 tttagccatt tgttggcaat aaagatcgac ttctgcgctt aaagctcgtt gcaacacact
     8941 gtaatcaacc gtttctgtcg gggatttctc atttccccgt ccccagtcgg gctgattcaa
     9001 ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcatc
     9061 ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg aagtggggaa ctacgccgtg
     9121 aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg
     9181 ttgctcaatg ggccgaataa catcggttaa cacccggcaa agggtggatt caagctcggc
     9241 aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca
     9301 gcaggtacga atctcgctta acggctccga atcatcctga aacaaggcgg ctaactgcgc
     9361 attcagcgca tcttgctgtt gacgcagaaa gtggttggcg gaggtcaggg ccagctcgat
     9421 ttcatgttta atctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc
     9481 ttcgacctga tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg
     9541 aaaatccagg tattcccgcg cctgctgcgc gtaattcaac agtttatgcg cggcagatcg
     9601 cagagcatac aacgaggcgt tagcgtaagc ggcatgaagc aacatctgaa ttggctgggc
     9661 gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcaa ggtccgccag
     9721 atcggcatga cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca cccagcgttg
     9781 ttgctctggc gctggtaact taccgttgtt ggctaactca tggcgcgccc gattcgccag
     9841 gtagccccac atcgacgaca ccggaaatat ctgctgtggc gtaatacagc ctttcatcag
     9901 cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc
     9961 gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg
    10021 gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga
    10081 ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg
    10141 cccgggggta tccagtaacg tcaattgacc gggataactc tccagccccg ccagatggac
    10201 aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc
    10261 gacgtccagc gccttcgcca gtcgcactaa atcattcaaa cttttcagac aatgaaaaat
    10321 aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag
    10381 cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg
    10441 caggcgctgt tgtaattgtt gaattaaaca atcgattggc gcgacatgtg aaaaatgcag
    10501 taccggttcc ttttgcccgg gcgtatggcg aataagcgtc ggcaacgcag tcattgggcg
    10561 attgcgatta ggcagtacct ccgtaccaac aatggcatta atggtggttg atttccctgc
    10621 tttcatagta ccgacaatcg ccagcaccat ttccagtcgg gaaattttac gcaactcatt
    10681 attcagcatc gcgtgacgct cggcgatatt aggctgactc cagggtaaag cccgttgtgg
    10741 cgcatcgtct ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga
    10801 aagctgtaac aggcgttcag cctcctgact taactcatac agggtctgtg tgtacataga
    10861 aaattcttcc ttaaagcaaa ttttattact tttatttagc cagattgttt ttgagttctg
    10921 ttt
//
LOCUS       DAZKFU020000155        10889 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZKFU020000155 DAZKFU020000000
VERSION     DAZKFU020000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.156"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            115..3405
                     /gene="yjiT"
                     /locus_tag="ACHOQQ_RS16435"
                     /old_locus_tag="pgaptmp_003287"
     CDS             115..3405
                     /gene="yjiT"
                     /locus_tag="ACHOQQ_RS16435"
                     /old_locus_tag="pgaptmp_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048814575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjiT"
                     /protein_id="HGU4471499.1"
                     /translation="MGQSEYISWVKCTSWLSNFVNLRGLRKPDGRPLYEYHATNDEYN
                     QLTQLLRAVGQSQSNICNKDFAACFVLFCSEWYRRDYERQCGWTWDPIYKKIGISFTA
                     TELGTIVPKGMDDYWLRPIRFYESERRNFLGTLFSEGGLPFRLLKESDSRFLAVFSRI
                     LGQYEQAKQSGFSALSLARAVIEKSALPTVFSEDTSVELISHMADNLNSLVLTHNLIN
                     HKEPVQQLDKVHPTWRSEFPIPLDDETGTHFLNGLLCAASVEAKPRLQKNKSTRCQFY
                     WSEKHPDELRAIVSLPDEVSFPVTSEPSTTRFELAICEDGEEVSGLGPAYASLENRQA
                     TVRLRKSEVRFVRQNPSAGLSLVARAGGMIVGSIKLDDSEIAIGEVPLTFIVDADQWL
                     LQGQASCSVRSSDVLIVLPRDNSTVAGFDGQSRAVNVLGLKALPVKGCQDVTVTANET
                     YRIRTGREQISIGRFALNGKRASWVCHPDETFIGVPKVISTLPDIQSIDVTRYLSGIS
                     IEQCHIQEMLGAQYLSIRNSNNETLLRRKIGILPADFSIEIKGGMHANEGTIVITTQH
                     PCVATLKDKTLEATRKRTEGRTEIQLKAEGVPPAFITLQVLPNLAADTIDIELPFPAK
                     GCLALDVNGRPLDKNITLHDLLGSRAFLFSRNGEPTKYTLQLHLRSISGLQAWHEWSY
                     TALSERPVELNLYSLREHIENLISLEAGIDQVVEMRITGAGVVMAWQIRRYKYSLRYD
                     YEKELLLSQSVNHRAGQIPSPVIMLLSEPERKSIPLVSRMSEGVPVGEYELSSIVNKN
                     GPWLVVPKPGEEMAFRPCFIRGESSLPVEESNIRSLQKATQLFNPQAEVNAITLVLGQ
                     MANDPAHSGWQFMRSLYDQFGYLPLATFEVWRALVQHPQALAMSLFKFEMSAEYLSRI
                     ENEFPILWEFFPIFEIKAASERFKLFLSQKGAPEETQKLLVTNMFQRLGLVFPTYADE
                     IEKWLSNGYLPPSIPESCVHGWYQELLREHSEARWPEYGCKRLYKWMMSQKNPVIDIN
                     PDANHRYSVAWLPVFAAAVASGNASFESVFDRKPGAVFFLRQVRDFDSRWFKAIFQCS
                     LLRYVAKK"
     gene            3418..9762
                     /locus_tag="ACHOQQ_RS16440"
                     /old_locus_tag="pgaptmp_003288"
     CDS             3418..9762
                     /locus_tag="ACHOQQ_RS16440"
                     /old_locus_tag="pgaptmp_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU4471500.1"
                     /translation="MTTRYFSSLIEQSLSRSTEATLSIMGVTNPKLREHLAQQMGADC
                     GKPGSFLASPVFQQMFGWKESNHTMRSLTEGNALLSKAVVDSLDDQSNGRYRFGADWK
                     PFTHQLASWKALLEKKHSVVVTSGTGSGKTECFMVPVLEDLYRELHENGNNPLVGVRA
                     LFLYPLNALINSQRERLDAWTRGFGTGIRYCLYNGNTENLHASVKSEQAKRPNEVLSR
                     EKMREEPAPILVTNGTMLEYMMVRQVDAPIIQQSKAQKSLRWIVLDEAHTYVGSQAAE
                     LALQLRRVMTAFGVTPDDVRFVATSATIAGSDAEKQLKKFLSELSGIAQERIDVLDGS
                     RVIPELEPCKNVSVPLDEIEQIPDLDLKDKNDNKIKGISPERFYALTHSPEARYIREM
                     LVSQPNPMKLDEMTQRLNALTKQHYSQQEVLRWIDVCSGTQPNAKDPAFLKVRAHIFQ
                     RNTQGIWACVDKNCRLKHGTPLEKGWPFGYVYVNQRQNCDCGSPVYEVAFCNDCNEPH
                     LLAQDKKGKLVQWENKGGDEFSLQDEVPVEHDATEEKVEKENSFQPPLIIAAGETSEA
                     GYTLQRLDRQTRRIGVINNDSIPLIINDVEQVCSASGCGYRGMSGKQPFRRALLGGPF
                     YVTNIVPTVLEYCQDFTSDEGKEGIGPDSLPGRGRRLITFTDSRQGTARMAVRMQQEA
                     ERSLLRGSVVEILSWHQRTQTSTAPNANADLEKLAARAKQAREQAEEYRSWGMPDQAK
                     LSQAQAEQLEQAYQAATGGKAATTLVSRTWTEMVNELKDKADIRGPVLKYNHYLQPEV
                     FNKNGGPLKLSEMLLFREFMRRPKRTNSLETQGLVQVGYQGLEKIHKSPLHWQEKGLT
                     LDDWRDFLKVTLDLYVRESNFTQLDDELKNWIGSRFSSKFVRNPESKDPEDNQNRRWP
                     QIRNGNVSHRLAKLLMLGAGFKTVNTATIDIINTWLKEAWAQLTGPLAVLKPDGNRFY
                     LPKEHMTFSLITDAWICPVTNKILDTAFKGLTPYLPTHISFEHLTQAQYDTFVAQKVT
                     MPEIWKLDRSQEDYAEGLAKARDWVNNDPLIAQLRSENVWTDINDRVVEGGFYYRTAE
                     HSAQQSSERLQSYEKMFKNGQLNVLNCSTTMEMGVDIGGITAVVMNNVPPHPANYLQR
                     AGRAGRSKESRAISYTLCKGNPHDQQVFANPLWPFETMIPAPMVAMNSARLVQRHVNA
                     LLLSDFLCNVIGETDKEKTSLDSLWFFGEDDGQSKCERFKVWLERPVLDIDTALERLV
                     KGTALHGARAEHLRDKTINAITFLQQRWLSVYRDLVTQERESQPQTPYRKRIELEKKR
                     HCGEYLLRDLAARTFLPGYGFPTDVVTFDNFTMEDYIREKSQKSRDKKDREDNVSRYK
                     GLPSRNLGVAIREYAPGAEIILDGRVFRSAGVSLHWHNINADTNEAQRLDCAWRCHKC
                     GTIGYEEGMSSSGMLFCSNSACGEKITMDNRRQVLQPAGFVTDAHAPVTNNIETMKFV
                     PVVPAWVFVKAEPVPLPNPLMGYMASGADGHVFQQSLGEGGHGYALCLSCGRAESMLN
                     ENDAPKSMEAHYPPRPGKADRDSQNHRLICPGSTALMKNVTLGALARTDVFEMVLRKP
                     QNGEYLPDNTEEGRIVAMTLAVALRQALAGVLGISAAELGYSVRPVRLEDGQSVLAVQ
                     LYDVISGGAGFASSAPVHIEAILQGMVKQLGCRHCDTACSECLLDSQTRHDHDLLDRK
                     VALAWLGDDFTYYIGLPDEETFSLPDARYCPGAIGDTIRRAINEGAEKLTLWMTGAPN
                     EWDLYARQFRAAIQSYRLKDNVEVDLVIPAGVDDPDLLHELSQFTALGVRLCHVEQEL
                     QLPIVAQVTFADRVMTLASRSQQATIPGPEWHLNDELVVRSLGYQTVELNEFILPAKA
                     ANAVERVKDIQIHKQLNGPLSQFGQRFWDVLFNDHEEAQSLMKNTRITGVHYTDRYLQ
                     NPVALALLGSILKPLKTKLTDGAEVALDTLFKDKDRPGNRPFHDWMSIADFQDFADQW
                     FAAALGRPIELTVFDSPRDIPHHRKLTVTFEDGQVLKIRFDQGMGYWRINFASQWHYF
                     DFRDDVSFQLVKMAQACKEGNVANSEESWATDVLVEVTAS"
     gene            complement(9805..10146)
                     /gene="symE"
                     /locus_tag="ACHOQQ_RS16445"
                     /old_locus_tag="pgaptmp_003289"
     CDS             complement(9805..10146)
                     /gene="symE"
                     /locus_tag="ACHOQQ_RS16445"
                     /old_locus_tag="pgaptmp_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HGU4471501.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPVAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVIAGKQKVA"
     gene            complement(10374..>10889)
                     /locus_tag="ACHOQQ_RS16450"
                     /old_locus_tag="pgaptmp_003290"
     CDS             complement(10374..>10889)
                     /locus_tag="ACHOQQ_RS16450"
                     /old_locus_tag="pgaptmp_003290"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4471502.1"
                     /translation="ILISLVGTIGKTLILSNLAETGIINPRLVKIALHSEIQRQYVKL
                     YLDGPLAYDFFKGFSHGGTMDILNLGILKELPIPLPSLEEQREIVRRVEQLFAYADTI
                     EKQVNNALARVNNLTQSILAKAFRGELTAQWRAENPELISGENSAAALLEKIKAERAA
                     SGGKKASRKKS"
BASE COUNT         2831 a         2500 c         2815 g         2743 t
ORIGIN      
        1 gaaaaattag ctattgccgc ctgattaatt atacgatatt tttaaaggtt ttctttcaaa
       61 ttagaaatat tctggggttt tgtttttgta tcttttatct ctaaggagct tgctttgggt
      121 caatcagaat acatttcatg ggtaaaatgt acttcctggc taagtaactt tgtgaatctt
      181 agagggttga gaaaaccgga tggccgtcct ctttatgaat atcatgcaac caatgatgaa
      241 tataaccaat taacgcagct actccgtgca gtcggtcaat cacaatctaa tatatgtaat
      301 aaggactttg ctgcctgttt tgttcttttt tgctctgagt ggtaccgcag ggattatgaa
      361 cggcaatgcg gatggacgtg ggatcctata tataaaaaaa tagggatttc atttaccgcc
      421 acagagcttg gtactatcgt accgaaaggg atggacgatt actggctgcg cccaattcga
      481 ttttatgaat ctgaacgacg caattttctc ggaacattat tcagtgaagg tggtttacct
      541 ttccggttac tcaaagaatc agacagtcgc tttctggctg ttttctccag gatccttgga
      601 caatatgagc aagcaaagca gtcaggtttt tctgcccttt cgctggcaag agccgtcata
      661 gaaaaatcag cgctgccaac tgtttttagc gaagatacct ctgtagaact tatcagtcat
      721 atggctgata acctcaattc cttagtactt actcacaacc taataaatca caaagaacca
      781 gtacaacagc ttgataaggt tcaccctacc tggcgaagcg agtttccaat accgttagat
      841 gatgaaacag gaacgcattt tttgaatgga ctgctatgcg cagcctctgt agaggctaag
      901 cctcgactac aaaaaaataa gagtacgcgt tgccaattct attggtcaga aaagcatcca
      961 gatgagctta gggcgatagt atctctccct gacgaagttt cctttcctgt aacaagcgag
     1021 ccgtcaacta cgcgctttga acttgccatt tgtgaagatg gtgaggaagt ctctggcctt
     1081 gggccagcct atgcttccct ggaaaacaga caggcaacag ttcgattacg taaaagcgaa
     1141 gtgagatttg tcaggcaaaa tccatcggca ggtttgtcgt tagtggctcg tgctggaggg
     1201 atgattgttg ggagcattaa acttgatgac agtgaaattg ccattggtga ggtgccgtta
     1261 accttcatcg ttgatgcaga tcaatggctg ttacagggac aggcctcctg cagtgtgcga
     1321 agcagcgatg ttctgattgt gctccctcgg gacaatagca ctgttgctgg ttttgatggc
     1381 caatcgagag cagtaaacgt attaggactt aaagcgctac ctgttaaggg atgtcaggac
     1441 gttacagtta cagcaaatga aacttatcgg attcgcaccg gaagagaaca aatcagcatc
     1501 ggaaggtttg ctctaaatgg aaagcgtgca agctgggttt gtcatccaga tgagacattt
     1561 attggtgtac caaaagtcat ttctacacta ccagatattc aaagcattga tgtaacgcgt
     1621 tacctgagcg gaatttcaat tgagcaatgt catatacaag agatgctagg ggctcaatat
     1681 ctttctatcc gtaatagtaa taatgaaacg ctcttgcgtc gcaaaatcgg tatcttgcct
     1741 gctgatttca gtattgagat aaaaggtgga atgcacgcaa atgaaggcac tatcgtaatc
     1801 accacacagc atccttgtgt agcaacactt aaagataaaa cattggaggc tacacgtaag
     1861 cgtactgagg ggcgaacaga gatacaactc aaagcggaag gggtacctcc ggcatttatt
     1921 acattacaag ttctgcctaa tttggcggct gataccatcg acattgagtt acctttccca
     1981 gctaaaggct gccttgctct tgatgtaaac ggacgtcctc tggataaaaa cattacgctg
     2041 catgatctgc tgggttccag agcattcctg ttctccagaa atggtgagcc gacaaaatat
     2101 acattgcagt tgcatttgcg ctccattagt ggattacagg catggcacga atggagttat
     2161 acggcattga gcgagcgtcc tgttgaattg aatctctata gtctgcgcga acatattgag
     2221 aacctgattt cacttgaggc aggtattgat caggttgttg agatgcgtat tacgggggcg
     2281 ggtgtggtca tggcctggca aattcgtcgc tataaataca gcctgcgcta tgattatgaa
     2341 aaagaactat tactcagcca gtcagtgaac catcgtgctg ggcagattcc ttcccccgtt
     2401 atcatgctgt taagtgaacc cgagaggaaa tccattccct tagtatctag aatgagcgag
     2461 ggagtgcctg tcggtgaata tgagttgagc tcaatcgtta acaaaaatgg accatggttg
     2521 gttgttccta aaccgggtga agagatggct tttcgtccct gcttcattcg tggggaatct
     2581 tcgttaccgg tagaagaaag caacatccgc tcattgcaga aagcgaccca gctttttaat
     2641 ccacaggcag aagttaatgc catcaccctg gtgctcggac agatggctaa cgatcctgcc
     2701 cattccggct ggcaatttat gcgtagcctg tacgatcaat ttggttattt accgctggca
     2761 acctttgaag tatggcgtgc actggtgcag catccgcagg cattggcaat gtcgctcttc
     2821 aaatttgaaa tgtcagcaga gtacctgagt cgtattgaga atgagttccc tattttatgg
     2881 gagtttttcc ccatctttga gatcaaagct gcatctgagc gttttaaatt gttcctgagc
     2941 caaaaaggtg caccggagga aactcagaag ttactggtta ctaatatgtt tcaacggctg
     3001 gggctcgtgt tcccgacata tgccgatgaa attgagaaat ggttaagtaa tggatatctc
     3061 cccccgtcta ttcctgaatc ttgtgtccac gggtggtatc aggagctctt acgtgagcac
     3121 agtgaggctc gctggcctga atatggctgc aagcgtctgt ataaatggat gatgtcacaa
     3181 aaaaatccag tgattgatat taatcctgac gcaaatcacc gctattccgt ggcctggtta
     3241 cccgtcttcg ccgcagcggt ggcaagcgga aatgcttctt ttgaatctgt atttgaccgt
     3301 aaacccggtg ccgtattttt cctgaggcaa gtcagggatt tcgattcccg ctggtttaag
     3361 gccatcttcc agtgcagcct gttgcgttat gttgctaaaa aataaaaagg aataaggatg
     3421 actacacgct atttttcgtc acttattgaa caatcgttgt ctcgttcaac tgaagccacg
     3481 ctcagtatca tgggcgttac aaacccgaag ttgcgtgaac atctcgcgca gcaaatgggg
     3541 gctgattgtg ggaaaccggg ctcattcctt gcttctccgg tattccagca aatgtttggc
     3601 tggaaggagt caaaccacac gatgcgcagc ctgacggaag gcaacgcatt gttgagcaaa
     3661 gcagtggttg attcactgga tgatcagagt aatggccggt accggtttgg tgctgactgg
     3721 aagcctttta cgcaccagtt agccagttgg aaagctttgc tggaaaagaa acattcggtg
     3781 gtggtaacca gtggtacggg ttcaggtaaa acggaatgtt ttatggttcc ggtgctggaa
     3841 gatctttacc gggaactgca tgaaaatggc aacaatccgt tggtcggtgt ccgagccctt
     3901 tttctctatc cattgaacgc cctgattaat tcacaacgcg agcgtctgga tgcctggacg
     3961 cgtggttttg gtaccgggat ccgctactgc ctttacaacg gcaacaccga aaacctacac
     4021 gcttcggtaa aaagcgaaca ggcaaaaagg ccgaatgagg tgctctcgcg cgaaaaaatg
     4081 cgtgaagaac ctgcgccgat tctggtgacc aacggcacga tgctggaata tatgatggtt
     4141 cgccaggtcg acgccccgat tatccagcaa tccaaagcac aaaaatcact acgctggatt
     4201 gtactggatg aagcgcatac ctatgtcggg tcgcaggccg ctgaactggc gctccagctg
     4261 cgtcgtgtga tgacagcgtt tggcgtgacg ccagatgacg tgcgctttgt ggcgacctct
     4321 gcgaccattg ccggcagcga cgctgaaaag cagctgaaaa aattcctctc tgaattatcc
     4381 ggcattgcgc aggagcgcat cgatgttctg gacggaagtc gggtgattcc tgaactggaa
     4441 ccatgcaaaa acgtttctgt tccgttagac gaaatagagc aaatacctga tctggatcta
     4501 aaagataaaa atgataataa aattaaaggg ataagtcctg agcgctttta tgcgcttacg
     4561 cactcccccg aagcgcgtta catacgtgaa atgttggtta gccaacccaa tccaatgaaa
     4621 ctggatgaga tgacgcagcg gttaaatgcg ctgacaaaac agcattacag ccagcaggaa
     4681 gtactgcgtt ggatcgatgt ctgttcaggg actcaaccta atgcaaagga tcccgcattt
     4741 cttaaagtca gggcgcatat cttccagcgt aatacccagg gaatatgggc ttgtgtggat
     4801 aaaaattgca gattaaagca cggtacaccg ctcgaaaaag gctggccctt tggctatgtg
     4861 tatgtgaacc agcgacaaaa ttgcgactgt ggaagccctg tctacgaagt ggcgttctgt
     4921 aatgattgta atgagcctca tcttctggcg caggacaaaa agggcaaact tgtccaatgg
     4981 gaaaataaag gtggcgatga gttctccttg caggatgaag tacctgttga acatgacgct
     5041 acagaagaaa aagtcgaaaa agagaacagt ttccagcctc cgttgattat tgccgcagga
     5101 gagaccagcg aggccggtta taccctacaa cgcctcgacc gccagacgcg ccgtattggc
     5161 gttattaaca atgacagcat tccgctgatt attaatgatg ttgaacaggt ttgtagcgcc
     5221 agtggctgtg gctacagagg catgagtggg aaacagccct tccggcgtgc actattaggt
     5281 gggccatttt acgttactaa tatcgtgccc accgttcttg agtattgtca ggactttacc
     5341 agtgatgaag gcaaagaggg catcggacca gattcgttgc cagggcgagg acgtcgactc
     5401 attaccttta cggacagtcg acaaggtaca gctcgaatgg cggtgcgtat gcagcaagaa
     5461 gcagaacgca gtctcttacg cggaagcgta gtcgaaattc tgagctggca tcaacggacg
     5521 caaacgtcca cggcgccgaa tgccaatgcc gatctggaaa aattagcggc cagggcgaag
     5581 caagcccgtg agcaggcaga agaatatcga agctggggaa tgccagacca ggcgaaattg
     5641 tcacaagcgc aggctgaaca gcttgaacag gcttatcagg ctgcaaccgg tggaaaagca
     5701 gcaactaccc tggtatcccg cacctggaca gaaatggtta acgagcttaa agataaagcc
     5761 gatatccgtg gcccggtact gaaatataac cactatttgc agcctgaagt gtttaataaa
     5821 aacggcgggc cactaaagct ttcggaaatg ttgttgttcc gggaattcat gcgtcgccct
     5881 aaacggacta acagcctgga aacacagggg ctggttcagg ttggttacca ggggctggag
     5941 aaaatacata agagcccctt gcactggcag gaaaaaggat taacgctgga tgactggcgc
     6001 gattttctca aggttacgtt ggatctttat gttcgcgaaa gcaacttcac acagctggat
     6061 gatgagctga aaaactggat tggtagccgt ttttcatcaa aattcgtccg taacccggaa
     6121 tcaaaagatc ctgaagataa tcagaacaga cgctggcctc aaattcgtaa tggcaacgta
     6181 tcccatcgtt tagcgaagtt actgatgctg ggggctggat tcaaaaccgt caatacggcg
     6241 actattgata ttatcaatac atggctgaaa gaagcatggg cccaacttac cggaccactt
     6301 gcagtactga aacccgatgg caaccgtttt tatttaccga aagagcatat gactttttct
     6361 ttaatcacgg atgcctggat ttgcccggta accaataaaa tcctggatac ggcttttaaa
     6421 ggcttaaccc cttatctgcc cacccatatt tcgttcgagc atcttaccca ggcgcaatat
     6481 gacacttttg tcgcgcagaa agtcaccatg ccggaaatct ggaagctgga tcgttcacag
     6541 gaagactacg cagagggtct ggcaaaagca cgagactggg tcaacaatga tccgctgatc
     6601 gcacaattgc ggtcggaaaa cgtctggaca gacatcaacg atcgtgtcgt ggaaggcggt
     6661 ttctactatc gcactgccga gcactctgcc cagcagtcgt cagaacgcct gcaaagctat
     6721 gaaaagatgt tcaagaacgg gcaactaaat gttctcaact gttccaccac catggaaatg
     6781 ggggttgata ttgggggcat tactgcggtg gtaatgaaca acgttccgcc acatccggcc
     6841 aactatttac agcgcgccgg gcgagccggg cgtagtaaag aatcacgcgc gatctcttat
     6901 accctatgca aaggtaaccc acacgaccag caggtttttg ccaacccact ctggccattt
     6961 gaaaccatga tcccggcacc catggtcgcg atgaactccg cccgactggt gcaacgtcac
     7021 gtcaatgcat tattactgtc tgacttttta tgtaacgtta ttggcgaaac cgataaagag
     7081 aaaaccagcc tcgatagcct ctggtttttc ggtgaagatg acgggcagtc aaaatgcgaa
     7141 cgctttaaag tctggctgga acgcccggtt cttgatatcg acacggcgct ggaacggctg
     7201 gtgaaaggta cggcgttgca tggcgcaaga gccgaacatc tgcgggataa aacaatcaat
     7261 gcgatcacct tcctgcaaca gcggtggctc tccgtttatc gcgatctcgt gacccaggag
     7321 cgtgagtcgc agccgcaaac gccttatcgt aagcgcatag agctggagaa gaaacggcac
     7381 tgtggcgagt atctgctgcg cgatctggct gccagaacct tcctgccggg atacggtttc
     7441 ccgacagatg tagtgacgtt tgataacttc acgatggaag attatatccg cgaaaaatcg
     7501 cagaaaagtc gtgataaaaa ggaccgtgaa gataacgtct cccgttataa aggattacct
     7561 tcccgtaacc tcggtgttgc cattcgtgaa tatgcaccgg gagcagagat cattcttgat
     7621 ggccgtgtct tccgctctgc cggtgtctca ctgcactggc acaacattaa tgctgatacc
     7681 aatgaagccc agcgtctgga ttgcgcctgg cgctgtcaca aatgcgggac cattggctat
     7741 gaagagggga tgagcagttc cgggatgctc ttttgctcca acagcgcctg cggcgaaaaa
     7801 ataaccatgg ataatcgccg tcaggtgttg cagcctgccg ggtttgtcac cgatgcacac
     7861 gctccggtaa cgaacaatat tgaaaccatg aaattcgtcc cggttgtccc cgcctgggtc
     7921 tttgttaaag cagagcccgt accgttgcct aacccgctaa tgggttatat ggcttctggg
     7981 gctgatgggc atgttttcca gcaaagtctg ggggaaggtg gacacggtta tgcattgtgt
     8041 ctgagctgtg gccgtgcgga gtcgatgctg aatgaaaatg atgctccgaa aagtatggaa
     8101 gctcactatc ctccgcgtcc gggtaaagcg gaccgcgaca gccagaatca tcgtctgatt
     8161 tgccccggtt cgacagcact catgaagaac gtcaccctgg gcgcactggc gcgcaccgat
     8221 gtctttgaaa tggttctgcg caaaccacaa aatggcgagt atctgccaga taacacagag
     8281 gaggggcgga ttgtcgccat gactctggcg gttgctctgc gtcaggcgct ggcaggcgtt
     8341 ctgggtatct ccgccgcaga actcggctat tctgtacgtc cggtcagact ggaggatggg
     8401 caatcggtat tagccgttca gctttatgat gtgatcagtg gtggtgcagg ttttgcctcc
     8461 agcgcaccgg tacacattga agctatattg cagggaatgg tgaaacagtt aggctgccgc
     8521 cattgtgata ctgcatgtag tgaatgcctg cttgattccc agactcgcca cgatcacgat
     8581 ttgctggatc gcaaagttgc gctggcctgg cttggcgatg acttcacgta ttacatcggt
     8641 ttaccggatg aagagacatt ctcgctgccg gatgcccgct actgccctgg cgctattggc
     8701 gatacgattc gccgggcgat taacgaaggt gctgaaaagc ttaccctatg gatgaccggc
     8761 gcgcccaatg agtgggattt atacgcccgt cagttccgcg cagctattca gagctatcgg
     8821 ctaaaagaca acgtcgaggt cgatcttgtt atccctgcgg gagtggatga cccggatcta
     8881 ttgcatgaac tttcgcaatt tactgcgctg ggtgtgcggt tgtgccatgt tgagcaggag
     8941 ctgcaattac cgattgttgc ccaggtcacc tttgccgatc gcgtgatgac gctggcctct
     9001 cgcagccagc aggcaacgat tccggggccg gagtggcacc tgaatgacga actggtggtg
     9061 cgtagtctcg gttatcaaac ggtagaactg aacgagttta ttctgcccgc taaagccgcc
     9121 aacgctgtcg agcgggtcaa agacattcag atccacaaac agttgaatgg accactttcg
     9181 cagtttgggc aacgcttctg ggacgtgctg tttaacgacc atgaagaagc gcagtcactg
     9241 atgaaaaaca cgcgaattac cggcgttcac tataccgatc gctatctgca aaacccggtt
     9301 gcgctggcgt tgttgggatc aattctgaaa ccgctaaaaa caaagctgac cgatggcgct
     9361 gaagtggcgc tggatacgct gtttaaagat aaagatcgcc ccggtaaccg gcctttccat
     9421 gactggatga gcatagcgga ttttcaggat ttcgctgatc agtggtttgc tgctgcgctg
     9481 ggcaggccga tagaactgac ggtgttcgat tcgccgcgcg atatccctca ccatcgtaaa
     9541 ctgacggtga cttttgagga tggtcaggta ttgaagatcc gcttcgatca ggggatgggc
     9601 tactggcgca tcaactttgc atcgcaatgg cattactttg atttccgcga tgacgtttct
     9661 ttccagttag tcaaaatggc tcaggcctgc aaggaaggga atgtggccaa cagcgaagag
     9721 agctgggcaa cggatgtgct ggtggaggtg accgcctcct gatgatgtgc cgctcccgac
     9781 gtcgtgtcgg gagcgttttt caattcaggc aactttctgt ttacccgcaa ttaccccaat
     9841 aaaatcctgc acctgctttt gtttacgcgc cgacagcttg cacacctggc gcagcgactg
     9901 catcagttcg ctctcttcgg cgacgggtgg ttgggcggtg aggacaatac agccttccat
     9961 tactttgaca tctaccgccg tgccggtggc aaaaccggcg gcttccagcc actgaccttt
    10021 cagggtaatg gcgggaatac ggctgtaatc cgggtagcgg ctcgcataac tcacggttaa
    10081 ttgacggtta tttgccgggg agacttctgc ttcgaacggt tgtgcaatag aatgcgtgtc
    10141 agtcatgatt actattctcc ataaatagtg attgtgatga gcggtgcggg tgtgttggcg
    10201 cacatccgca ccgcgctaaa tacctggata tatcatcagt aaatacagga aaagtccagc
    10261 taaaaataga ataaaatggg caatttctgg aatgaataaa atatatttgc gtgagttatg
    10321 attggcgtga aataataaaa agcgcaccgg aaaggtgcgc cagaaaataa tgatcaggat
    10381 tttttacgcg aggctttttt acccccgctg gctgcgcgtt cagctttgat tttctccagc
    10441 aacgcggcgg cgctgttttc accgctgatt aattccgggt tttcggcccg ccactgggca
    10501 gtgagctcac cacggaatgc ttttgccagg atggattgcg tcagattgtt gacgcgggct
    10561 aaggcgttgt tgacctgttt ttctatggtg tcggcgtagg cgaagagttg ctcgacgcgg
    10621 cgaacgattt cgcgttgttc ctccagcgat gggagaggaa taggtaactc ttttaatata
    10681 ccaagattta ggatatccat agtcccgcca tgactgaatc ctttaaaaaa gtcatatgct
    10741 aaggggccat ctaaatacag ctttacatat tggcgttgaa tttcactatg taatgctatt
    10801 tttactaggc gtggatttat tatacctgtc tctgcaagat tagatagtat taatgttttt
    10861 ccgatagtac caactaaact aataagaat
//
LOCUS       DAZKFU020000156        10869 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZKFU020000156 DAZKFU020000000
VERSION     DAZKFU020000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.157"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /gene="ecpR"
                     /locus_tag="ACHOQQ_RS16455"
                     /old_locus_tag="pgaptmp_003291"
                     /gene_synonym="matA"
     CDS             <1..285
                     /gene="ecpR"
                     /locus_tag="ACHOQQ_RS16455"
                     /old_locus_tag="pgaptmp_003291"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGU4471503.1"
                     /translation="NYWYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDIKKDKIT
                     DREMEIIRMTAQGMQPKSIARIENCSVKTVYTHRRNAEAKLYSKIYKLVQ"
     gene            360..947
                     /gene="ecpA"
                     /locus_tag="ACHOQQ_RS16460"
                     /old_locus_tag="pgaptmp_003292"
                     /gene_synonym="matB"
     CDS             360..947
                     /gene="ecpA"
                     /locus_tag="ACHOQQ_RS16460"
                     /old_locus_tag="pgaptmp_003292"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGU4471504.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1005..1673
                     /gene="ecpB"
                     /locus_tag="ACHOQQ_RS16465"
                     /old_locus_tag="pgaptmp_003293"
                     /gene_synonym="yagY"
     CDS             1005..1673
                     /gene="ecpB"
                     /locus_tag="ACHOQQ_RS16465"
                     /old_locus_tag="pgaptmp_003293"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGU4471505.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1699..4224
                     /gene="ecpC"
                     /locus_tag="ACHOQQ_RS16470"
                     /old_locus_tag="pgaptmp_003294"
                     /gene_synonym="yagX"
     CDS             1699..4224
                     /gene="ecpC"
                     /locus_tag="ACHOQQ_RS16470"
                     /old_locus_tag="pgaptmp_003294"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HGU4471506.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSMWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4214..5857
                     /gene="ecpD"
                     /locus_tag="ACHOQQ_RS16475"
                     /old_locus_tag="pgaptmp_003295"
     CDS             4214..5857
                     /gene="ecpD"
                     /locus_tag="ACHOQQ_RS16475"
                     /old_locus_tag="pgaptmp_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGU4471507.1"
                     /translation="MRVNLLITMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5826..6536
                     /gene="ecpE"
                     /locus_tag="ACHOQQ_RS16480"
                     /old_locus_tag="pgaptmp_003296"
     CDS             5826..6536
                     /gene="ecpE"
                     /locus_tag="ACHOQQ_RS16480"
                     /old_locus_tag="pgaptmp_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGU4471508.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6849..7178
                     /locus_tag="ACHOQQ_RS16485"
                     /old_locus_tag="pgaptmp_003297"
     CDS             6849..7178
                     /locus_tag="ACHOQQ_RS16485"
                     /old_locus_tag="pgaptmp_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4471509.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7389..8003)
                     /locus_tag="ACHOQQ_RS16490"
                     /old_locus_tag="pgaptmp_003298"
     CDS             complement(7389..8003)
                     /locus_tag="ACHOQQ_RS16490"
                     /old_locus_tag="pgaptmp_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HGU4471510.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8050..8259)
                     /locus_tag="ACHOQQ_RS16495"
                     /old_locus_tag="pgaptmp_003299"
     CDS             complement(8050..8259)
                     /locus_tag="ACHOQQ_RS16495"
                     /old_locus_tag="pgaptmp_003299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471511.1"
                     /translation="MMMGAGLLLELYDAKTDKETCDCQLCITLQIYVTSNIKIGRNDG
                     AYILWRLDNHLVIQNNTVLYIYLVL"
     gene            8421..9110
                     /gene="paoA"
                     /locus_tag="ACHOQQ_RS16500"
                     /old_locus_tag="pgaptmp_003300"
     CDS             8421..9110
                     /gene="paoA"
                     /locus_tag="ACHOQQ_RS16500"
                     /old_locus_tag="pgaptmp_003300"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HGU4471512.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9107..10063
                     /gene="paoB"
                     /locus_tag="ACHOQQ_RS16505"
                     /old_locus_tag="pgaptmp_003301"
     CDS             9107..10063
                     /gene="paoB"
                     /locus_tag="ACHOQQ_RS16505"
                     /old_locus_tag="pgaptmp_003301"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HGU4471513.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPDAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10060..>10869
                     /gene="paoC"
                     /locus_tag="ACHOQQ_RS16510"
                     /old_locus_tag="pgaptmp_003302"
     CDS             10060..>10869
                     /gene="paoC"
                     /locus_tag="ACHOQQ_RS16510"
                     /old_locus_tag="pgaptmp_003302"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGU4471514.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVML"
BASE COUNT         2763 a         2899 c         2842 g         2365 t
ORIGIN      
        1 aattattggt attacaacag caatattaga ggcgtggtat acgctggact gagtcgtgat
       61 attagaaaag aactggccta tgtgattaat ggcaggttcc tgagaaaaga tattaagaaa
      121 gataaaatca ctgaccggga aatggaaatt atccgcatga cggcccaggg aatgcaacct
      181 aaatcgattg ccagaattga aaattgtagt gtgaagacag tgtataccca tcggcgtaat
      241 gctgaggcca agctgtactc aaaaatatat aagttggttc agtaaactcc aggcaagtta
      301 gttttaaaaa atgactcact gggacatcac gtcctcaatt caactcggga agaaatgcaa
      361 tgaaaaaaaa ggttctggca atagctctgg taacggtgtt taccggcatg ggtgtggcgc
      421 aggctgctga cgtaacagct caggctgtag cgacctggtc ggcaacagcc aaaaaagaca
      481 ccaccagtaa gctggttgtg acgccactcg gtagcctggc gttccagtat gccgaaggca
      541 ttaaaggttt taactcacag aaaggtctat ttgacgtggc tatcgagggt gactcaacgg
      601 ctaccgcctt taaactgacc tcacgtctta tcaccaacac attaacccag ttggatacct
      661 caggttccac actgaatgtg ggcgtggatt ataacggcgc ggcagtcgaa aaaactggcg
      721 ataccgtgat gatcgatacc gccaacggcg tactgggcgg caaccttagc ccgctggcta
      781 acggttacaa tgccagcaat cgtaccaccg cacaggatgg tttcaccttc accatcatca
      841 gcggcaccac caatggtacc accgcagtaa ccgactacag cactctaccg gaaggcatct
      901 ggagcggcga cgttagcgta cagttcgacg cgacctggac cagttaatct ctctgatgta
      961 ccagcagggg tagcccccct gctttattcc tggacggaat tgttatgaaa aagcaccttc
     1021 tgcctctcgc tctgctgttt tccggaatat ctccggccca ggcgctggat gtcggcgata
     1081 tatcatcgtt tatgaacagt gacagcagca cgctgagcaa aacgatcaaa aacagtaccg
     1141 acagtggtcg ccttatcaat atccgtctcg aacggctctc ttcaccgctt gacgacgggc
     1201 aggttatctc aatggacaag ccggatgagt tgctactcac tcccgccagc ttgctgctac
     1261 ccgcccaagc cagcgaagtg atccgcttct tctataaggg acccgcagat gaaaaagagc
     1321 gctactaccg cattgtctgg tttgatcagg ccctcagtga tgcgcagcgc gataatgcca
     1381 accgcagcgc tgtggccact gcttccgccc gcatcggcac cattctggtc gtcgcccctc
     1441 gccaggcgaa ctaccacttt cagtacgcca acggctccct gacaaataca ggaaatgcga
     1501 cgctgcggat cctcgcctac ggcccttgcc tgaaagccgc caacggtaag gagtgtaaag
     1561 agaattacta cctgatgccg ggcaagtcgc gtcgttttac ccgcgtggac actgcggata
     1621 acaaaggacg ggttgcactt tggcagggtg ataagttcat tcccgtgaaa tagatagctg
     1681 tgcagatgga taacgacaat gcctttacga cggttctccc caggactgaa agcccagttt
     1741 gccttcggca tggtcttttt gttcgttcag cccgatgcca gcgctgctga cataagtgcg
     1801 cagcaaatag gtggggtgat tattccgcag gccttcagtc aggcgcttca ggacggcatg
     1861 agcgtcccgc tctatattca tctcgccggt agccagggtc gccaggacga tcagcgaatc
     1921 ggcagcgctt ttatctggct ggatgatgga cagctacgca tccggaaaat acagctggaa
     1981 gagagtgaag ataacgccag tgtcagcgaa caaactcgac agcagctgat ggctctggcg
     2041 aacgccccgt tcaatgaggc ccttaccatc cccctgactg acaacgcgca gctggatctc
     2101 agcttgcgcc aactgctgct gcagctggtg gtcaagcgcg aagcgctggg caccgtacta
     2161 cgctcacgta gcgaagacat cgggcagtcc agtgttaaca ccctcagcag taatctgagc
     2221 tataacttgg gcgtctataa caaccagttg cgtaacggcg ggagcaacac atccagctat
     2281 ctgtcgctga ataacgttac tgcgctgcgc gaacatcatg tggtgctcga cggctcgctg
     2341 tacgggatcg gtagcggtca acaggacagt gaattatata aagcgatgta tgaacgcgat
     2401 tttgccggtc accgatttgc cggtggaatg ctcgacacct ggaacttgca gtccttaggg
     2461 ccgatgaccg ccatttcagc agggaagatt tacggccttt cctggggaaa ccaggccagc
     2521 tccaccatct tcgacagcag ccagtcagcc acgccagtga tcgccttttt accggcggcg
     2581 ggtgaagtac atctcacccg tgatgggcgg ctactaagcg ttcagaactt cactatgggc
     2641 aatcatgaag tggatacccg gggtctaccg tacggtattt acgatgtgga agttgaggtg
     2701 atcgttaacg gtcgcgtgat cagcaaacgc acccagcggg tcaataagct gtttagccgg
     2761 gggcgcggcg ttggtgcacc actggcgtgg caggtatggg gcggtagctt tcatatggat
     2821 cgctggtcgg aaaacgggaa aaagacgcga ccagctaagg agagctggct agcaggtgcc
     2881 tcgacctccg gctcattgag tacgcttagc tgggcggcaa cgggatatgg atacgataat
     2941 caggcggtgg gtgaaacccg tctgacgctg ccgcttgggg gagcgatcaa cgttaacctg
     3001 caaaatatgc tggccagtga cagctcatgg agcaacatcg ccagcatcag cgccactcta
     3061 ccgggaggct ttagttcgat gtgggttaac caggaaaaaa cccgcattgg caatcaattg
     3121 cgacgtagcg atgccgacaa ccgtgcaatc ggcggcacac tcaacctgaa ctcactgtgg
     3181 tcgaagctgg gcacattcag catcagctac aatgatgacc gccgttacaa cagccattat
     3241 tacacagcag attactatca aaatgtctac agcggtccct ttggttcgct tggcctgcgg
     3301 gccggtattc agcgctataa caacggcgac agcaacgcca atacagggaa atatatcgct
     3361 ctcgatctct cgctaccact gggcaactgg tttagcgcag ggatgactca tcaaaacggc
     3421 tacaccatgg caaacctgtc agcacgcaag cagtttgatg aagggaccat tcgcactgtt
     3481 ggtgccaatc tgtctcgagc catctccggc gataccggtg atgacaaaac tctcagtggt
     3541 ggggcgtatg cacagttcga cgctcgctac gccagcggaa cgctgaacgt caatagcgcg
     3601 gcggacggct acgtcaatac caatttaacc gccaatggca gcgtcggctg gcagggtaaa
     3661 aacattgctg ccagcgggcg gactgatggc aacgctgggg tgatattcaa caccgggctg
     3721 gaggacgacg gtcagatcag cgccaaaatc aacgggcgga ttttcccact taacggcaag
     3781 cgtaactatc tcccactctc tccctatgga agatatgagg tggagttaca gaacagcaaa
     3841 aactcactcg acagttacga tatcgtcagc ggccgcaaaa gccatctgac tctctatcca
     3901 ggcaatgtcg ctgtcattga gccagaggtg aagcagatgg ttaccgtctc cggtcgtatc
     3961 cgtgcggaag acggcacact gctggctaac gcacggatta acaaccatat cggccgaacc
     4021 cgaaccgatg aaaacggcga gtttgtcatg gacgtggata agaaataccc cactatcgat
     4081 tttcgctaca gtggcaataa aacctgcgaa gtggctctgg aactcaacca ggcgcgcggc
     4141 gccgtctggg tcggtgatgt ggtctgcagc ggcctctcat cgtgggcggc ggtgacgcag
     4201 acaggagaag agaatgagag ttaacctact aataacgatg ataatctttg cgctaatctg
     4261 gcctgcaacc gcgctcagag ctgcagtgag caaaacaacc tgggcggatg caccggcacg
     4321 cgagtttgtg tttgtcgaaa acaactcaga cgacaacttt ttcgtcactc ctggcggggc
     4381 gctggatccg cgcctgaccg gtgccaaccg ctggaccggt ttaaaataca atggttcagg
     4441 aaccatctat cagcaaagcc tcggctacat tgataacggt tacaacaccg gcctctatac
     4501 caactggaag tttgatatgt ggctggaaaa ttcgccggtt tcatctcctt taactggctt
     4561 acgctgcatc aactggtacg cagggtgtaa tatgaccacc agtcttatcc tgccgcaaac
     4621 caccgacgcc agtggatttt atggcgcgac ggtgaccagc ggcggcgcga agtggatgca
     4681 cggcatgttg tcagacgcgt tttaccagta tctgcaacaa atgcccgtcg gcagcagctt
     4741 tacaatgacc atcaatgcct gccagacctc tgtgaactat gacgccagca gcggcgcacg
     4801 ctgtaaggat caggcctccg gcaactggta tgttcgcaac gtcacccata cgaaagcagc
     4861 aaatctacgg ttgataaata cccactcgct ggcggaagta tttatcaaca gcgacggagt
     4921 accgactctg ggcgaaggga acgccgactg ccggacgcaa accatcggca gccgttcagg
     4981 attaagttgt aagatggtta actataccct gcaaacaaac ggactcagca acacctcaat
     5041 ccatatattc ccggcgatcg ccaactcgtc gttagcctcg gccgtcgggg cgtacgatat
     5101 gcagttcagt ctgaatggca gttcatggaa accggtgagc aataccgcct attactacac
     5161 cttcaacgag atgaagagcg cagactcgat ctatgttttc ttctcgagca acttctttaa
     5221 gcagatggtg aacctcggga tcagcgatat caacaccaaa gatctattca actttcgctt
     5281 tcagaacacc acatcaccgg agtctggctg gtatgaattt tctacctcca acacgctgat
     5341 tatcaaaccc cgtgatttca gcatcagtat tatctccgat gaatatactc agacaccgtc
     5401 gcgggaggga tatgttggca gcggcgagtc ggcactcgat ttcggctata tcgtaaccac
     5461 cagcggtaaa acagctgccg acgaagtgct gatcaaggtg accggacccg cgcaggtgat
     5521 tggcgggcgc tcctattgtg tcttcagctc cgatgacggt aaggcgaaag taccgttccc
     5581 ggcgacactt tcctttatta cccgcaacgg agctacaaaa acctacgatg ccgggtgcga
     5641 tgatagctgg cgggatatga ccgatgcgct gtggttgacc acaccgtgga ctgatatctc
     5701 tggcgaagtg gggcagatgg ataagaccac agtcaaattt tcgattccaa tggataacgc
     5761 catttcgctg cgtacggtag atgataacgg ctggtttggc gaagtcagcg cttcaggaga
     5821 gattcatgtt caggcgacgt ggcgtaacat taactaaggc cctgctgaca gcggtctgta
     5881 tgctggcggc acctttgaca caggcgattt cggtcggcaa tctgacattt tcgctgccgt
     5941 ccgagactga ctttgtcagc aaacgtgtag tgaataacaa caaaagcgca cggatatacc
     6001 gtattgccat cagtgctatt gatagcccgg gcagcagtga attgcgcacc cgaccggtgg
     6061 atggtgaact gcttttcgcc ccccgccagc tggcgttgca ggctggtgag agcgagtatt
     6121 ttaaatttta ctatcatggt ccacaggata accgcgagcg ctactaccgg gtctcatttc
     6181 gcgaggtccc cactcgtaac cagacaagac gcagcccaac cggcggcgag gtcagcacgg
     6241 agccggtggt ggtgatggat accattctgg tagtacgacc acgtcaggtt cagtttaaat
     6301 ggtccttcga tcaggtgacc ggaacggtaa gtaacaccgg caacacgtgg tttaagctac
     6361 tgattaagcc aggatgtgat tcgaccgaag aggaaggcga tgcctggtat ctacgtccgg
     6421 gagacgtggt tcatcagcct gagttacgtc agccggggaa tcattatctg gtctataacg
     6481 acaaattcat taagattagc gattcctgtc cggctaagcc cccttcggcg gactgatact
     6541 tatccatcgg tcgagggaag aattccacct cagagactca aaaaacgttt aaatgatgtg
     6601 gaagcgatcg tcaatgtcac ctgtaacgat gggctttctc atatgcacac catagtgttc
     6661 aggagacaca gaaaggtatc aggctgcctg ctaacgagtg gtttattcac gaaatagtac
     6721 gtaaaatcgg taacggctgg aaatcattca atactcacac tatcgaaagt acaccagcca
     6781 accgcggtac gttcttacat acgatgtacc gctgttctct ttacgattta tagctgtact
     6841 ggtgaattat gagcaatctg aatccatgca tgacgtgtgg tgcctgttgt gcatttttcc
     6901 gcgtctcttt ttactgggcc gaagccgacg atgctggcgg taccattccc gccaggctca
     6961 ctgaacaaat atcccctttt caccgatgca tgagcggtac caatcagaaa aacccccgat
     7021 gtattgccct tgcaggaacc ccaggcaaaa atgcctgctg cacgatatat aaaaatcgat
     7081 cgtccacgtg cagagaattc gccatgtctg gtgagaacgg agaagtcaat gaggcttgca
     7141 atcgtgcaag ggctaaatac gggctgacac cgttataaat atacaataat taattgcact
     7201 gccccgccag cgataatagc ggggcttcgt tttcagggac aacaaaaccc gaaacttctt
     7261 gtatttgcca gcaacaatgc ctctcttctg tagcgttctt cggatctaca tcatcctaaa
     7321 gtagcgcgat atactcactg actatctgaa tcagaatata aaaagcaatg tttttatcat
     7381 tcaatgcatt atctgtttga gcctaagggg atctcagggt ctggctcatg agtaattctg
     7441 tttcgtaaat ctctgcgaat aatttcaatg gaccagaacc agactaaatg tccaaaaatt
     7501 tcagaaacat tctcatacca cgggagatca aaaagaggtg gtgtcagtcc catgagaggg
     7561 aatgaaatca tatgaacaaa aagttgggct aaagcacctg ccagtaagcc ctgccagagt
     7621 ttaatttttg gaaatacttc agcgaccaca caataaccga cagcaaacac tatcgaaaag
     7681 ataatgtgcg taacaccaac ccagttaaag acatgcccgg caaaggtata aacagccgca
     7741 ttgggatctg tcagccccag ccagtctcga agaaaaatat acggaggatt gagaaaatta
     7801 cgcgagcaat caatttgccc agcagccctg attaatgatt ccgggccaca cgctgcatta
     7861 aacatatcca ccgggctacg tggcggcaat ggaacttcag ccccccactt cacaaatgcg
     7921 gaaacaacgc cagcaatcag cccaatgaat gcagcaagac cataacgtct gcggttcggt
     7981 ggagtttgtt caaatatatt catatctacc ctgcttgtac cattatgtta tacacctctt
     8041 taggagtatt cataaaacaa ggtaaatgta aagaactgta ttgttttgta taacaagatg
     8101 gttgtctaat cgccaaagaa tataagcacc atcatttctc cctattttta tattagaagt
     8161 gacatagatt tgcagggtga tgcagagctg acaatcacag gtttccttat cggtttttgc
     8221 atcgtacaac tcaagcaata aaccagctcc catcatcatc aacatcccgg cgaacatgaa
     8281 tttattagcc gggctcatag ctgcattcgc gttttaagaa taatcctcct gctgtcgccg
     8341 actatgctta acgtttaaaa aagcatcagc actctcgcaa cgcactctta ttttcccctt
     8401 tagaataccg gaggcctggt atgagcaacc aaggcgaata ccccgaagat aatcgggttg
     8461 ggaagcacga gccgcacgat tttagtttga cccgtcgcga tctgattaaa gtgagcgccg
     8521 caacggcggc gaccgccgtg gtttatcctc attctacgct tgcggcaagc gttccggcag
     8581 ctacacccgc gccagagata atgcccctga cacttaaggt gaacggcaaa acggagcagc
     8641 ttgaggtgga tacccgaacc acgctactgg acgctttgcg tgaaaatctg catttgatcg
     8701 gtaccaagaa aggttgcgat cacggacagt gcggagcctg taccgtgctg gtcaatggtc
     8761 gcaggcttaa tgcctgcctg acgctggcag tcatgcatca gggggccgag atcaccacca
     8821 ttgaaggcct gggttcacca gataatcttc accccatgca ggcggccttt atcaagcatg
     8881 atggcttcca gtgcggctac tgcacctccg ggcaaatttg ctcatcagta gcggtgctaa
     8941 aagagattca ggacggcatt cccagtcacg tcacggtcga tttggtttcc cctccagaaa
     9001 gaactgccga tgagatccgt gaacgtatga gcggcaacat ctgtcgctgt ggtgcatacg
     9061 ctaacatcct tgccgccatt gaagacgctg cgggggagat aaaatcatga aggcgtttac
     9121 ctatgaacga gtgaacaccc cggccgaggc ggcacttagc gctcagcgcg tacccgacgc
     9181 aaaatttatc gcgggcggga ccaatctgct ggacctgatg aagctggaaa ttgaaacgcc
     9241 cacccacctt atcgatgtga acggcctcgg gctcgataag attgaagtga ccgacgcggg
     9301 tgggctgcgc atcggcgcac tggtacggaa caccgacctg gcggctcacg agcgcgtgcg
     9361 tcgtgattac gcggtactct cccgcgccct gctcgctggc gcgtctggtc agttacgcaa
     9421 tcaggcaacc accgcaggta atctgctcca gcgcacgcgc tgcccctatt tttacgacac
     9481 caatcagccc tgcaataagc gcctgcccgg gagcggctgc gcggcgcttg aaggctttag
     9541 ccgtcagcac gcggtggtag gcgtaagcga agcctgcatt gccacccatc cgagcgatat
     9601 ggcggtcgca atgcggttgc tggatgcggt ggtggaaacc atcacgccgg agggaaagac
     9661 tcgcagtatc acactggctg atttttatca cccaccgggg aaaacgccgc acattgaaac
     9721 cgccctgctt cccggtgagc ttatcgttgc ggtgacgtta cctccgccgc tcggcggaaa
     9781 acatatttac cgtaaggtgc gcgatcgcgc ctcctacgcc tttgccctgg tatcggtcgc
     9841 ggcgattatt cagcctgacg gcagcgggcg cgtcgcgctg ggcggagtag cacataagcc
     9901 ctggcgcatt gaggctgcgg atgctcagct atcccagggg gcacaggccg tgtatgacgc
     9961 gctgttcgcc agcgcccatc ccaccgctga aaacaccttt aaactcctgt tggcgaagcg
    10021 aacgcttgcc tccgtactgg ctgaagcgag ggcacaggca tgaaatttga taaacccgca
    10081 ggggaaaacc cgatcgatca gctgaaggtt gtcggtcgtc cccatgaccg catcgacgga
    10141 ccgctgaaaa ctaccggcac ggcacgctac gcctacgaat ggcatgaaga atcccccaac
    10201 gccgcctatg gctatatcgt cggttccgcc attgccaaag gacgcctcac cgcccttgat
    10261 acggacgccg cgcaaaaagc gccgggcgta ctggctgtca ttaccgccag taacgccggg
    10321 gcactcagta aaggcgacaa aaacaccgcc aggctgttag gcggccccac tattgagcac
    10381 tatcatcagg ccattgcgct ggtagtggcc gagaccttcg aacaggcgcg agcggcggcc
    10441 tcgctggtgc aggcgcacta tcgccgtaat aaaggagctt actccctggc ggacgaaaaa
    10501 caggccgtca gtcagccgcc ggaagacacg cccgacaaaa acgtcggtga ctttgacggg
    10561 gctttctcct ccgctgcggt gaagattgat gctacctaca cgaccccgga tcagagccat
    10621 atggcgatgg agccgcatgc ctcgatggcc gtctgggatg gaaataagct tactctctgg
    10681 acctcaaatc agatgattga ctggtgccgc accgatctgg caaaaacgct gaaagtgccc
    10741 gtggagaatg tgcgtattat ctccccgtat atcggcggag ggtttggcgg caagctgttc
    10801 ctgagaagcg atgcgctgct ggcagccctc gccgcccgag cggtgaaacg tccggttaaa
    10861 gtgatgctc
//
LOCUS       DAZKFU020000157        10802 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZKFU020000157 DAZKFU020000000
VERSION     DAZKFU020000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.158"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..463)
                     /gene="yobD"
                     /locus_tag="ACHOQQ_RS16515"
                     /old_locus_tag="pgaptmp_003303"
     CDS             complement(5..463)
                     /gene="yobD"
                     /locus_tag="ACHOQQ_RS16515"
                     /old_locus_tag="pgaptmp_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGU4471515.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(518..1369)
                     /gene="manZ"
                     /locus_tag="ACHOQQ_RS16520"
                     /old_locus_tag="pgaptmp_003304"
     CDS             complement(518..1369)
                     /gene="manZ"
                     /locus_tag="ACHOQQ_RS16520"
                     /old_locus_tag="pgaptmp_003304"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGU4471516.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1382..2182)
                     /gene="manY"
                     /locus_tag="ACHOQQ_RS16525"
                     /old_locus_tag="pgaptmp_003305"
     CDS             complement(1382..2182)
                     /gene="manY"
                     /locus_tag="ACHOQQ_RS16525"
                     /old_locus_tag="pgaptmp_003305"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGU4471517.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2245..3216)
                     /gene="manX"
                     /locus_tag="ACHOQQ_RS16530"
                     /old_locus_tag="pgaptmp_003306"
     CDS             complement(2245..3216)
                     /gene="manX"
                     /locus_tag="ACHOQQ_RS16530"
                     /old_locus_tag="pgaptmp_003306"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGU4471518.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3533..3691
                     /gene="yoaL"
                     /locus_tag="ACHOQQ_RS16535"
                     /old_locus_tag="pgaptmp_003307"
     CDS             3533..3691
                     /gene="yoaL"
                     /locus_tag="ACHOQQ_RS16535"
                     /old_locus_tag="pgaptmp_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGU4471519.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3679..5235
                     /gene="yoaE"
                     /locus_tag="ACHOQQ_RS16540"
                     /old_locus_tag="pgaptmp_003308"
     CDS             3679..5235
                     /gene="yoaE"
                     /locus_tag="ACHOQQ_RS16540"
                     /old_locus_tag="pgaptmp_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGU4471520.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(5239..6837)
                     /gene="pdeD"
                     /locus_tag="ACHOQQ_RS16545"
                     /old_locus_tag="pgaptmp_003309"
     CDS             complement(5239..6837)
                     /gene="pdeD"
                     /locus_tag="ACHOQQ_RS16545"
                     /old_locus_tag="pgaptmp_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4471521.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6968..8332)
                     /gene="sdaA"
                     /locus_tag="ACHOQQ_RS16550"
                     /old_locus_tag="pgaptmp_003310"
     CDS             complement(6968..8332)
                     /gene="sdaA"
                     /locus_tag="ACHOQQ_RS16550"
                     /old_locus_tag="pgaptmp_003310"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4471522.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8516..9094)
                     /gene="nudL"
                     /locus_tag="ACHOQQ_RS16555"
                     /old_locus_tag="pgaptmp_003311"
     CDS             complement(8516..9094)
                     /gene="nudL"
                     /locus_tag="ACHOQQ_RS16555"
                     /old_locus_tag="pgaptmp_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGU4471523.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(9098..10459)
                     /gene="pabB"
                     /locus_tag="ACHOQQ_RS16560"
                     /old_locus_tag="pgaptmp_003312"
     CDS             complement(9098..10459)
                     /gene="pabB"
                     /locus_tag="ACHOQQ_RS16560"
                     /old_locus_tag="pgaptmp_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGU4471524.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10533..10712
                     /gene="yoaH"
                     /locus_tag="ACHOQQ_RS16565"
                     /old_locus_tag="pgaptmp_003313"
     CDS             10533..10712
                     /gene="yoaH"
                     /locus_tag="ACHOQQ_RS16565"
                     /old_locus_tag="pgaptmp_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGU4471525.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         2694 a         2738 c         2763 g         2607 t
ORIGIN      
        1 taagttattg agttgatacg agaagcttat aaattttttc cagatcgtcg atatttcgaa
       61 cgcggattaa cagccgacgc tgttctaatt gcatcaccaa cacgccatct tccgacaagt
      121 tcatcgcttt gattcggcta tattcaatcc agacattggc gaagaaaaaa cctttttgtt
      181 taaagatgat cttcggaacg cggatccaga atatataaaa acccatcaga gccagtgcgc
      241 ttaataacca tgtcgttatt aacgcaccat gattcgtgac gttgttatag ataagaatga
      301 caatcaatcc gacgaagata acgctatcga tgcgaccacg ccggagcaaa ggaattgcca
      361 gcagggtggg gccgttacgg cggggcatga tgaactgatc gtagatcgcg aaggccagga
      421 gtgcggcgat gaaaagaatc agtaccaggt ccgtgattgt cattaatcct ccagataaaa
      481 aaacggggcc aaaaggcccc ggtagtgtac aacagtctta cagtcccagc aggccgcaag
      541 cgtaaccagc gataccgatg acgaagaagc caacgatgat ccacagcggg ttaacttttt
      601 tgcgcagtag ccacatacaa gcaaaggtca gcagcagtgg taccaggcct ggcattaact
      661 ggtccagaat agtctggaca gtagtaacgt gttctttgcc cgtctggtca gtaatgcgag
      721 agacaaccag cgggatgttg acatgtgtcc acttgttaac caatgccccc atgacaaaca
      781 ggccgaggat agacgcccct tccgtcagtt tttgcaggaa gccaccaccc atatctttaa
      841 cgatatcgat acctttggag taaccatacg ctacgccgta gtaacgggtt gccagacgca
      901 ccaggttaaa caggatgaag aacagcagcg gacctaacag gctgccgctc atcgcgatac
      961 cggcacccag tgctgcaaat accggacgta cggttcccca gaagatcggg tcgcctacac
     1021 cagccagcgg ccccatcaaa ccgactttga taccgttgat agcaccgtca tcgatctctg
     1081 cgccattagc acgctgttct tccagcgcca gggttacgcc gagaatcggc gcagccacga
     1141 acggctgggt gttaaagaac tccaggtgac ggcgaatagc ttgtttacga gcttcgttgt
     1201 tctcagggta gaggcgacga attgccggta ccatagagaa gcagaaaccc agtgcctgca
     1261 tacgttcgaa gttccatgaa ccctggaaga ggttagaacg caggaagacg ccacgaatat
     1321 cactttgagt gagttttttc tcggtggtag tttgagttgt atcaaccatt tcgctcacct
     1381 gttagtccag ttcgttatcg agatcgttgt taccagcagc ctgagcaggc gcaccggcta
     1441 cgcggttgta tttcgggcta agttggatgt agagcactgc cataacagta ccgatcacac
     1501 ccagagcaac caggttaaag ttggtgaatg ctgcggttac gaagccgagg tagaagaacg
     1561 gcatcaggta gccagcacgc atcatgttga taaccatcgc ataaccaacc accacgatca
     1621 tgccaccggc gatattcaga ccattggtca ccacttccgg aatcgcattc agcatgttct
     1681 gtacttcgct ggtaccaaca gacagcgcaa cgatgacggc cggaatagcc acacgcattg
     1741 cttgcaggaa cagagaagaa acgtggatcc aggaaatcgc tgtcaggttg ccgttatcag
     1801 cagccttatc cgcagcgtgc tggaaagcaa cggtaatagt acgaacgatg atggtcagta
     1861 cctggcccgc agcggccaga gggattgcca gtgcaatacc tgcaccaatg ctctgatgac
     1921 ctgcgataac cagaatggta gaaatgatag atgccagagc ggcgtcaggc gcaactgcag
     1981 caccgatgtt catccagccc agcgcgatca tttccagcgt accaccgata ataataccgg
     2041 ttttcatatc cccaagaacg atacccacca gggtacacgc gattagcgga cggtgaaact
     2101 gaaattcatc gaggattgat cccatacctg cgatacaggc tacgataaat accagcacaa
     2161 tttgaagagt ggtaatctcc attgtacttc tcctgtttac gtaagactaa agtgtgaaaa
     2221 ctgagtgata atcaacacaa tacgttactt atcgattttg ctgatcagat ccatcatttt
     2281 cagtttcgga tcggtggaaa ccttacggac ttccagctca ataccgcgtg cattcagttt
     2341 tttgaacgcc tcgatatctt tttcatcaac cgaaaccgcg ttattcacct gggttttacc
     2401 ctgacggaat gccataccac cgacgttaac agaggtgatt tttacgccgc cttcaacgag
     2461 acgctctaca tctgttgggt tggtaaataa cagcattaca cgttcgccag catatttcgg
     2521 gttgttgtag acgcgaatca ttttggcaac atcaactacg tgtgctgtta cgcccggagg
     2581 tgcaacctgg gtgagcagtg tcttacgaac ggtatccgca gccacttcat cactaacaac
     2641 aataatacgg gagacattgg tttctttggt ccagcgggtg gcgacctgac cgtgaatcag
     2701 acggtcgtcg atacgcgcaa ggccaataac catgtagtcg tttggcccca ttggttttgc
     2761 cggagttgga gccgcttttg gtgctgcggc agcgggtgct ggcgcggctt tttcaaccgg
     2821 tttggctttc agcgctttca cgccttcacg gcctgtttct actgccagcg caaccagttc
     2881 atcaaagctt gggtcatcat cacgggccat taacgtttcc acgagcattg gaatgttaac
     2941 gcctgcaatg acttcataat gctctttgtc gacgacaatg cggctggcag cattgaacgg
     3001 gctgcctccc catgtatcaa cgagaaacag cacgccttta gtggtgtcga gttttgccaa
     3061 ctgagcgttg tacttttcaa tcagcgtttc ggcattttca cctggaacga aatcgatcca
     3121 gccgacgttt tcctgctcgc ctaacagcat ttctgccgtt ttaagcaact gctctgcagc
     3181 ccaaccatgt gtgcctataa caatagcaat ggtcacttgc tacctccttt attatcgtta
     3241 acacctcaac gtgccagatg tatttttgaa tcgtcgtcca caatcgaatc gattcagata
     3301 agggctacaa ccaaaaaccc ctgcgtttcg cgatttattt tagatatcga aaaaattatt
     3361 ttatgtgatg aagatccgta atttaacttt cgattagcag aaatttcgaa aggtaaaata
     3421 tccttgtcac attcgtttgc aaaggaaggt aaatctttgc caaatcagag gcgtctctga
     3481 tatgtttaac tcccgtttaa caaccatgga gtatagggca gtagcccgca gtatggatcg
     3541 tcaccgacgt catttcagca tcaggccttt taacgcctgc ctttctggca ctctatgccg
     3601 cacctttcgt ttgcattttg tcgttacgcc tgcattattt ctggcgtcga atagctattc
     3661 cttaagcagg agcttgtcat ggaattctta atggacccct caatttgggc ggggctactc
     3721 acgcttgttg ttctcgaaat tgtgctgggt atcgataacc tggtcttcat cgccattctt
     3781 gctgacaaac tgccgccaaa acaacgcgat aaagcgcgtt tgctggggtt atcactggcg
     3841 ctgattatgc gtctggggct gctgtcgctg atttcatgga tggtcacgct gaccaaaccg
     3901 ctatttaccg tcatggattt ctccttctcc ggacgcgacc tgattatgtt gttcgggggg
     3961 atattcttgc tgttcaaagc aacaaccgaa ctgcatgaac ggctggaaaa ccgcgatcat
     4021 gattccggcc acggtaaagg ctacgccagt ttctgggtag tcgtcacaca gattgtcatc
     4081 cttgacgccg tcttctcgtt ggatgcggta attactgctg tagggatggt taaccatctg
     4141 ccggtgatga tggcggcggt agtgattgcg atggcggtta tgttgctggc atccaaaccg
     4201 ctgacgcgat ttgttaacca gcaccccacg gtggtggtgc tctgtctgag cttcctgtta
     4261 atgattggtc tgagtctggt ggcagaaggt ttcggtttcc acattccgaa aggttacctg
     4321 tatgccgcga ttggcttctc gatcatcatc gaagtgttta accagattgc gcgtcgcaac
     4381 tttattcgtc accagtcgac tttgccgctg cgagcgcgta ctgccgatgc catcctgcgt
     4441 ttgatgggcg ggaaacgtca ggccaatgtt cagcacgatg ccgataaccc gatgccgatg
     4501 ccgatcccgg aaggtgcatt tgccgaagaa gaacgttaca tgattaacgg cgtactgacg
     4561 ctggcgtcgc gttctctgcg cgggatcatg acgccgcgcg gtgaaataag ctgggttgac
     4621 gctaatctcg gggtcgatga aatccgcgag cagctgctct cctcaccgca cagcctgttc
     4681 ccggtatgtc gcggtgaact ggatgaaatc atcggaatcg tacgtgctaa agaactgctg
     4741 gtggcgctgg aagagggcgt tgatgtggcg gcgattgctt cggcgtctcc ggcgattatc
     4801 gtccctgaaa ccctcgatcc gatcaacctg ctgggcgtgc tgcgtcgtgc tcgcgggagc
     4861 tttgttatcg tgaccaacga gtttggtgtg gtacaaggct tggtcacgcc gctggatgtg
     4921 ctggaagcca ttgcgggtga attcccggac gctgacgaaa cgccggaaat cattactgat
     4981 ggtgacggct ggctggtaaa aggcggtaca gatttgcatg ccttgcagca ggcgcttgat
     5041 gttgagcacc ttgccgatga cgatgatatc gcgacggtcg cgggcctcgt gatctcggca
     5101 aatggtcaca ttccccgtgt gggggatgtg attgatgtag ggccactgca tatcaccatc
     5161 attgaagcca atgattatcg tgttgatctg gttcgcattg ttaaagagca accggcgcac
     5221 gatgaagatg agtaagcgtt aacgtaatgg cataatgggc gtgatatgtc cattatgccg
     5281 ggcgggcggc ggttggctgc ccgccagcca tttgggaaaa tcacgtagcg gcatcggctg
     5341 tgcgtacaaa tacccttgca aaatatgcac gccatgacgg cgcaaatact tcgcttgttc
     5401 ttgcgtttcc acgccctccg ccaccagttc aatattcagt ctttgcccca gcgcgatgat
     5461 gatatcggtc accgtcgagt taaccgcgtc agaacctata gctgcggtaa atgacttatc
     5521 aattttcagc acgtcaggac gtaatgtttc aagccaggaa aatgaactgt tgccggtgcc
     5581 gaagtcatca atcgccagtt tgacattttt acgatgcagc tcgcgggcaa tccgataatc
     5641 aacatccagt aaggcatcgc gttcggtgat ttcgaggatc agttgctgaa tcgggtgagc
     5701 gctaaaccag tactgattga gatcttttat cagcacacca cggcgaaaat ggctgggtgc
     5761 gacgttaatg ccaacatgaa actgactact catcgggaaa acatggcgct gacgaatggt
     5821 ttctgccatc acatagcggg tcagtggcac aattaaatga tgttcttccg cgataggaat
     5881 aaacacatcc ggtgaaatcc agccctgacg cggattgttc cagcgcagca aaatctctac
     5941 accaatacat tgctggctgc gcgcattaag caaaggctga cagaacaatt cgaactcatg
     6001 ttgcgccagg cccagattga tttcgcggga aaagctcatc cggtaagcgg tcgccagcca
     6061 ggcgatgtag cccaccagta aacttagcag caccgccagc ggtaattgtg ttggaagata
     6121 gtgccatgcc agcgccgtag cccccggacc attaacgtta atggtaaaag gaaagcgttg
     6181 cgaagaaacc tgatagtttt cattgtcttc aggttgcgga agggaatcta ccagcccatt
     6241 accatagagc aaatgccgtt tgtccaccgt caggctggcg ctactgattt gcggcagttg
     6301 tggctcaagg agcattgccg tcagtaagtc gatgttaatc atctccatga ccccagcatt
     6361 gctactgccc gctgcaggcg tccattgaat caaaaccgga ctgcctttaa tcagggcacg
     6421 gtcgatcgtc aggcgtaaaa gtggttgcgg tgcaggaagt tcagccagaa tgtccacgac
     6481 gggcacattg cgataaccaa aaatgctgga gcaatagagt gtgccgtctt gcaccaggcc
     6541 aatggatcgc acagtttgga gttttgccgc ctgtttacgt aatggcaaat gggcgacaga
     6601 gcagggcaga ccaatcagcg gaagcaagac ttcgctacct gcctgtaggg gaagcagtac
     6661 tttatctaat tcctctacag catgattggc gaattgtact acccgttgtt gatttaagtt
     6721 acgctgtgaa ataaatcgca cgctcagggt cgaagcgagc gtaacgagtg cgcaaatcgt
     6781 acaaacaatc attcgattac ggcgataggt tttaatgatc cgttgtgctt tttgcatgcg
     6841 ggccaccctg taagcgtatg gcgacaaacg cctgacgcaa cagtggaagt gtagtgggga
     6901 atattacagc agacgagaaa gcggggataa attcgcccat ccgttgcaga tgggcgagta
     6961 agaagtatta gtcacactgg actttgattg ccagaccacc gcgtgaggtt tcgcggtatt
     7021 tggcgttcat gtccttaccg gtttcgtaca tcgtttcgat gaccttatcc agcgagacgc
     7081 gcggtgcact ggtgcggcgc agagccatcc gtgcggcgtt aatcgccttc acagatgcaa
     7141 tggcattacg ctcaatgcac ggcacctgaa cctgccctgc aaccgggtcg caggttaaac
     7201 caaggttgtg ttccatgcca atttccgccg ccacgcaaac ctgttccggg ctaccgccca
     7261 gcagttctgc aagacccgca gcagccattg aacaggcaac acccacttcg ccctggcaac
     7321 caacttccgc accggaaata gaggcgttca ttttatacaa tgcaccaatc gcgcccgctg
     7381 ccataaagta acgggtatag atgtccgggc tgaccgattc aataaagtgg tcatagtaag
     7441 ccagcactgc cggaacgata ccgcaggcac cgttggttgg cgcagttacc acacgaccac
     7501 cggcggcgtt ttcttcgtta actgccagcg caaacatgtt tacccagtca atgacattca
     7561 tcggatcgtt agacagttta tcgctggaaa ccagcatccg gcgcagggca gacgcacgac
     7621 gcggcacgcg cagcgggcct ggcagtacac cttcggtgtt catcccgcga tcgatacatg
     7681 cctgcatggt ttgccagaca tgcgcgaaat actcgtcgat ctctttcttg ctgtgcagcg
     7741 ccagttcgtt ctgcatagcg agaccagaca gcgaatagcc ggtttcatta cagtacgcga
     7801 gcagttcggt ggcagatttg aacggatacg gcacgctcac ttcgttggca gcatcctgac
     7861 caaagtgttc ttcatcgacg ataaaaccgc cgccgatgga ataataagtt ttgctgtaga
     7921 cgacttcatc gccgttatag gcgtggattt gcataccgtt ttcatgcagc ggcaggttgc
     7981 cgttatgaaa acgcatcccg ttgtcgcgcg ggaaatccac ttcatgccgt ccctgtgcca
     8041 gcagcagacg ttcgcgctct tctacgtcgc gaataaaacc gggaatactg tcgatatcca
     8101 cggtggcagg ttcgttacct gcaagaccca taataatggc gatatcggtg tggtggcctt
     8161 tacccgtcag cgacagtgaa ccataaacgt ccacggcaac gcgagtaacg ctatccagta
     8221 agcctttttc gaccagatca tcgacgaact gtttacctgc cttcataggc cctacggtat
     8281 gggaagatga gggaccaatc cccaccttaa acatgtcgaa tagactaatc acgataatac
     8341 tcctgacaag gtgactggac ttccagtaac gaactaataa cagcgcatag tgtaagaggg
     8401 aacgcagcga ctggcttaac tattcacatg aattaaacta atcaaaaacc gcgatttaac
     8461 taatattgcg atgattgtct caagtagtgt tttgtagatg taaagataag tatagtcagg
     8521 gtttcacacc aatttgcagc gccagctcac gaattatgcc tgcggtcatt ccccatacaa
     8581 aatactgttc gtaccaggac agccataccc gatgtgaatc gccacggcgg tagatatcta
     8641 aagggtgata acgacccaga tgtaatgcct gggcgagcgg catttcaaac accgccgaga
     8701 cttcatcttc actggcgcga tacggcagat cgggcgggat aatgccgacc actggggtta
     8761 cctggtagcc agttacgcta tcgacgggcg gcagcacgcc gataacttca acggcggaag
     8821 gcggtatagc aacctcttct tcagcttcgc gcagcgcggc ggcgataact gatgcgtccg
     8881 tgtcatcgac tgcacctcca gggaatgcca cttgtccagc gtgtttacgc agatgaattg
     8941 aacgctgagt cagcaacaac cccggttgcg gtcgacggac gatggggatt aacacggcag
     9001 cctgacgatg atttagggtt tcccggttaa tttgcgggcg caaaagttga aagcgcgata
     9061 aaaaatcatc aagcgtcagg ctacggtatt ccacgtctta cttctccagt tggcgtaata
     9121 tcttattaac tttatcaaaa gtttcctgat attccgcttc ttcctggcta tcggcgacaa
     9181 ttccaccgcc cgcagagcag tatatttgtc cgttaatggc agtcagagtg cggatagtaa
     9241 tgctggtatc catgttgccg caaaagctca aatagccaat gctgccgcac caggcattac
     9301 gtcgctgcgg ttccagttcg tcgataattt ccatagcccg tactttcgga gccccggtta
     9361 ttgagccacc aggaaaagct gcgcgcagca gatcgctggc gtgtaactgt tctggtagtt
     9421 gcgccgttat ggtgctgacc agatgatgca cggcagggaa gggttccacc acgaacagct
     9481 ctggtacttt taccgaacct gctacggcaa cacgaccgat atcattacgc attaaatcga
     9541 caatcatcag attttcggca cgatctttcg ctgagttcgc cagtttttct gcttgtttgc
     9601 tatcttcctg aggatcgggc aggcgtggta gcgtgccttt aatcggtcgg gtctggattt
     9661 cactattatc acaaaggata aaccgctctg gcgaaaggct taaaattgca ccctgttcaa
     9721 gacgtaaaaa agcgctaaat ggcgcgcggt tggcctgatt aagctgaagg aatgcctgcc
     9781 attcatcgcc agaataggtc gcatgaaaac gctgggcgag attcacctga tagcaatcac
     9841 cgctgtgcag atattcctgt acctggcgaa atttttcgcc gtactgctcg cgggtcatat
     9901 tggattgcca gtcggaagtg agcgtgaaat cttcctgcgg cgagaattgc tggctttcca
     9961 gccaggcccg acgggcattg acatcattat gactcagcaa agaaactgta tgacgctggt
    10021 ggtcgacaat gagcgcccaa tcgtagatac ccactgccat atccggcaga acgatatctt
    10081 gttccgcaat ttctggcagt gactcaaaac ggcggcccag atcgtagcca aacaacccca
    10141 gtgcgccgcc ctgaaatggc aaatcttcgt tatgcgttgg gcgaatgtct gcgcgatcca
    10201 gcacctgctg gagcacctgt agcgggtcat cagtggtcgt tgtgcgtttt tcgctttcac
    10261 taacaacggt ttctttacct aaagtggtta aagtgcaaat cggatcggcg accacaatat
    10321 caaagcggct atacggatga tcggcatagc cggagtgtaa aagcatcgcc cacggcaggt
    10381 ggcttaagcg ggagaaataa aattcagcgg cgtcctgacg ccagggtaaa gtaatcacag
    10441 cgggagataa cgtcttcatc agtcctgact ctactggcta tgtgccgggt agcatgaaat
    10501 aattagcgcc aacaatttag caggagttaa caatgtttgc aggtttacct tcactcaccc
    10561 atgaacagca gcaaaaagct gtcgagcgga tccaggaact gatggcacaa gggatgagca
    10621 gcggccaggc aatcgcgctg gtggcagaag agctgcgtgc caaccactcc ggcgagcgaa
    10681 tcgtggcgcg ttttgaggat gaagacgagt aacacactaa tccgtatcga tttcgttata
    10741 gaagccgatg attatttgat tgtgagaaaa aggacgcttt ttatatgtga ttaaaagcgt
    10801 cc
//
LOCUS       DAZKFU020000158        10799 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZKFU020000158 DAZKFU020000000
VERSION     DAZKFU020000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.159"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            170..1312
                     /gene="yghO"
                     /locus_tag="ACHOQQ_RS16570"
                     /old_locus_tag="pgaptmp_003314"
     CDS             170..1312
                     /gene="yghO"
                     /locus_tag="ACHOQQ_RS16570"
                     /old_locus_tag="pgaptmp_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HGU4471526.1"
                     /translation="MERDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1319..2083)
                     /gene="glcC"
                     /locus_tag="ACHOQQ_RS16575"
                     /old_locus_tag="pgaptmp_003315"
     CDS             complement(1319..2083)
                     /gene="glcC"
                     /locus_tag="ACHOQQ_RS16575"
                     /old_locus_tag="pgaptmp_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HGU4471527.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLSASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2334..3833
                     /gene="glcD"
                     /locus_tag="ACHOQQ_RS16580"
                     /old_locus_tag="pgaptmp_003316"
     CDS             2334..3833
                     /gene="glcD"
                     /locus_tag="ACHOQQ_RS16580"
                     /old_locus_tag="pgaptmp_003316"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HGU4471528.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHVGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSGEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3833..4885
                     /gene="glcE"
                     /locus_tag="ACHOQQ_RS16585"
                     /old_locus_tag="pgaptmp_003317"
     CDS             3833..4885
                     /gene="glcE"
                     /locus_tag="ACHOQQ_RS16585"
                     /old_locus_tag="pgaptmp_003317"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HGU4471529.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4896..6119
                     /gene="glcF"
                     /locus_tag="ACHOQQ_RS16590"
                     /old_locus_tag="pgaptmp_003318"
     CDS             4896..6119
                     /gene="glcF"
                     /locus_tag="ACHOQQ_RS16590"
                     /old_locus_tag="pgaptmp_003318"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HGU4471530.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKSDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6124..6528
                     /locus_tag="ACHOQQ_RS16595"
                     /old_locus_tag="pgaptmp_003319"
     CDS             6124..6528
                     /locus_tag="ACHOQQ_RS16595"
                     /old_locus_tag="pgaptmp_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HGU4471531.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6550..8721
                     /gene="glcB"
                     /locus_tag="ACHOQQ_RS16600"
                     /old_locus_tag="pgaptmp_003320"
     CDS             6550..8721
                     /gene="glcB"
                     /locus_tag="ACHOQQ_RS16600"
                     /old_locus_tag="pgaptmp_003320"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HGU4471532.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMASGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9076..10758
                     /gene="glcA"
                     /locus_tag="ACHOQQ_RS16605"
                     /old_locus_tag="pgaptmp_003321"
     CDS             9076..10758
                     /gene="glcA"
                     /locus_tag="ACHOQQ_RS16605"
                     /old_locus_tag="pgaptmp_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HGU4471533.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2512 a         2733 c         3039 g         2515 t
ORIGIN      
        1 ggtcgcatta aatccacaat agtcatgttg atcggctaac ggtggcgctg gtgtatcgta
       61 attattcata aatattattt tatttttcct tatattcatt gcgtggatag ttaaggtacc
      121 gtccgttgct gtttattttc agcattaatt atttgttatc cactaagcta tggaacgcga
      181 tctgctgatg attaaaattg aaaaagtaat taataaaaat gaccttaagg catttatcgc
      241 ttttccgtca tcactttatc ccgacgatcc aaactggata ccccctttat tcattgaacg
      301 caacgagcat ttgtctgcga aaaatccggg gacggaccat atcatctggc aggcgtgggt
      361 ggcaaaaaaa gcggggcaga tagttgggcg tattaccgcg caaatagata ccttgcaccg
      421 cgagcgttac ggcaaagata ccggtcattt cggcatgatt gacgccattg atgatccgca
      481 ggtttttgcc gcgttgtttg gcgcagcgga agcgtggttg aagtcacaag gtgcaagtaa
      541 gatcagcggt ccattcagcc tgaatatcaa tcaggaaagc ggattactga ttgaaggttt
      601 cgatacacca ccctgtgcga tgatgccaca cggcaaaccg tggtatgccg cgcatattga
      661 acaactgggt tatcacaaag gcattgattt actggcgtgg tggatgcagc gaaccgatct
      721 cactttctct ccggcgctaa aaaaactgat ggatcaggtg cgcaaaaagg tgaccattcg
      781 ctgcatcaat cgtcagcggt ttgccgaaga gatgcagatc ctgcgtgaga ttttcaactc
      841 tggctggcag cacaactggg gatttgtgcc gtttaccgaa catgaattcg cgaccatggg
      901 cgaccaactt aaatatctgg tgccggacga tatgatctac atcgctgaga ttgattctgc
      961 accctgcgcg tttattgtcg gcttgccgaa catcaacgag gcgattgccg atctgaacgg
     1021 atcgctcttt cccttcggct gggcaaaatt gctgtggcgt ttgaaagtca gcggtgtgcg
     1081 aactgcgcga gtaccgctga tgggcgtacg cgacgagtat cagttcagcc gcatcggccc
     1141 ggtgattgcg ctgttattga ttgaagcctt acgcgatccg tttgcccgcc ggaagatcga
     1201 tgcgctggag atgtcatgga tcctcgaaac caatacaggc atgaataaca tgctggaacg
     1261 cattggggcg gagccgtaca agcgttaccg attgtatgaa aagcagattt aataccagct
     1321 aactcaggtt catctccagc ggcaccgccg agcgaatcaa atggtggcct tccagctcga
     1381 tatcgtggag attctttttc acggtccgca catgatcgcg tgctgcgcgc tgggcgacgt
     1441 gcggcagccg ctgcaacacc gcgttgtaga tccgcgcatg ctggcgatcg atctgctttt
     1501 tttgctgtgg tcgatggtag agattattta cgctggcaaa cactgaatta aacatcagat
     1561 cggtcaatga ttgcagcgta aacaccagca cctgattgtg agaagcctga caaatggcga
     1621 gatggaaagc gtgatccaac tgcgcatgtt cgatcagcga aatctctttg ttgttctcac
     1681 tggcggagag cattttttca taacagcgag ttatcacaac aaaatcagcc tgcgttccca
     1741 gcgttgccgc cagccttgcc gattcgccct ccagtaatgc gcgaacatcg agcagatcgt
     1801 acagcgttcg cggttgcgta ctgaacagat ggatcagcgg actggtgtcc tgcacccgat
     1861 taagccgtgc gacacgagaa tcgcgaccct gcgccgtttc aataatcccg cgcccgcgca
     1921 gcacggtcag cccttcacgc agtgcggagc gtgagaagcc gagcttttca cacagtcgac
     1981 gttccgaggg aagcggctga ccgaccttca gtacgccgtc gataattaac cgttcgatac
     2041 tctctgcaac cacttcgcaa atagggcgac gttcatcttt cattcccgga cctcgtgcac
     2101 aggtaggacc aatttttctg ttcttaactc gcaaaacacg cacatcacgt aagtgattat
     2161 gttacatcaa tttaacatta agttaaccaa ggcaaggtca cagagctgga aaaaaaatgg
     2221 tctgaccggt agctaaagag atagacgaaa acgaaaagcc cgcttaataa ctgttcacag
     2281 aagcagcgcg caaaaatcag ctgccacaca acataacaaa gcgaagccta ctcatgagca
     2341 tcttgtacga agagcgtctt gatggcgctt tacccgatgt cgaccgcaca tcggtactga
     2401 tggcactgcg tgagcatgtc cctggactgg agatcctgca taccgatgag gagatcattc
     2461 cttacgagtg tgacgggttg agcgcgtatc gcacgcgtcc attactggtt gttctgccta
     2521 agcagatgga acaggtgaca gcgattctgg ctgtctgcca tcgcctgcgt gtaccggtgg
     2581 tgactcgtgg tgcaggcacc gggctttctg gtggcgcgct gccgctggaa aaaggtgtgt
     2641 tgttggtgat ggcgcgcttt aaagagatcc tcgacattaa ccccgttggt cgccgcgcgc
     2701 gcgtgcagcc aggcgtgcgt aacctggcga tctcccaggc cgttgcaccg cataatctct
     2761 actacgcacc ggacccttcc tcacaaatcg cctgttccat tggcggcaac gtggcagaaa
     2821 atgccggcgg cgtccactgc ctgaaatatg gtctgaccgt acataacctg ctgaaaattg
     2881 aagtgcaaac gctggatggc gaggcactga cgctgggatc ggatgcgctg gattcgcctg
     2941 gttttgacct gctggcgttg ttcaccggat cggaaggtat gctcggcgtg accaccgaag
     3001 tgacggtaaa actgctgccg aagccgcccg tggcgcgggt tctgttagca agctttgact
     3061 cggtagaaaa agccggactt gcagttggtg acatcatcgc caatggcatt atccccggcg
     3121 ggctggagat gatggataac ctgtcgatcc gcgcggcgga agattttatt catgtcggtt
     3181 atcccgtcga cgccgaagcg attttgttat gcgagctgga cggcgtggag tctgacgtac
     3241 aggaagactg cgagcgggtc aacgacatct tgttgaaagc gggcgcgact gacgtccgtc
     3301 tggcacagga cgaagcagag cgcgtacgtt tctgggccgg tcgcaaaaat gcgttcccgg
     3361 cggtaggacg tatctccccg gattactact gcatggatgg caccatcccg cgtcgcgccc
     3421 tgcctggcgt actggaaggc attgcccgtt tatcgcagca atatgattta cgtgttgcca
     3481 acgtctttca tgccggagat ggcaacatgc acccgttaat ccttttcgat gccaacgaac
     3541 ccggtgaatt tgcccgcgcg gaagagctgg gcgggaagat cctcgaactc tgcgttgaag
     3601 ttggcggcag catcagtggg gaacatggca tcgggcgaga aaaaatcaat caaatgtgcg
     3661 cccagttcaa cagcggtgaa atcacgacct tccatgcggt caaggcggcg tttgaccccg
     3721 atggtttgct gaaccctggg aaaaacattc ccacgctaca ccgctgtgct gaatttggtg
     3781 ccatgcatgt gcatcacggt catttacctt tccctgaact ggagcgtttc tgatgctacg
     3841 cgagtgtgat tacagccagg cgctgctgga gcaggtgaat caggcaatta gcgataaaac
     3901 gccgctggtg attcagggca gcaatagcaa agccttttta ggtcgccctg tcaccgggca
     3961 aacgctggat gtgcgttgtc atcgcggcat tgttaattac gacccgaccg agctggtgat
     4021 aaccgctcgt gccggaacgc cgctggtggc gattgaagcg gcgctggaga gcgccgggca
     4081 aatgcttccc tgcgagccgc cgcattatgg tgaagaagcc acctggggcg ggatggtcgc
     4141 ctgcgggctg gcggggccgc gtcgcccgtg gagcggttcg gtccgcgatt ttgtcctcgg
     4201 cacgcgcatc attaccggcg ctggaaaaca tctgcgtttt ggtggcgaag tgatgaaaaa
     4261 cgttgccgga tacgatctct cacggttaat ggccggaagc tacggttgtc ttggcgtgct
     4321 cactgaaatc tcaatgaaag tgttaccgcg accgcgcgcc tccctgagcc tgcgtcggga
     4381 aatcagcctg caagaagcca tgagtgaaat cgccgagtgg caactccagc cattacccat
     4441 tagtggctta tgttacttcg acaatgcgtt gtggatccgc cttgagggcg gcgaaggatc
     4501 ggtaaaagca gcgcgtgaac tgctgggtgg cgaagaggtt gcgggtcagt tctggcagca
     4561 attgcgtgaa caacaactgc cgttcttctc gttaccaggt accttatggc gcatttcatt
     4621 acccagcgat gcgccgatga tggatttacc cggcgagcaa ctgatcgact ggggcggggc
     4681 gttacgctgg ctgaaatcga cagccgagga caatcaaatc catcgcatcg cccgcaacgc
     4741 tggcggtcat gcgacccgct ttagtgccgg agatggtggc tttgccccgc tatcggctcc
     4801 tttattccgc tatcaccagc agcttaaaca gcagctcgac ccttgcggcg tgtttaaccc
     4861 cggtcgcatg tacgcggaac tttgaggagc aggctatgca aacccaatta actgaagaga
     4921 tgcggcagaa cgcgcgcgcg ctggaagccg acagcatcct gcgcgcctgt gttcactgcg
     4981 gattttgtac cgcaacctgc ccaacctatc agcttctggg cgatgaactg gacgggccgc
     5041 gcgggcgcat ctatctgatt aaacaggtgc tggaaggcaa cgaagtcacg cttaaaacac
     5101 aggagcatct cgatcgctgc ctcacttgcc gcaattgtga aaccacctgt ccttctggtg
     5161 tgcgctatca caatttgctg gatatcgggc gtgatattgt cgagcagaaa gtgaaacgcc
     5221 cactgccgga gcgaatactg cgcgaaggat tgcgccaggt agtgccgcgt ccggcggtct
     5281 tccgtgcgct aacgcaggta gggctggtgc tgcgaccgtt tttaccggaa caggtcagag
     5341 caaaactgcc tgctgaaacg gtgaaagcta aaccgcgtcc gccgctgcgc cataagcgtc
     5401 gggttttaat gctggaaggc tgcgcccagc ctacgctttc gcccaacacc aacgcggcaa
     5461 ctgcgcgagt gctggatcgt ctggggatca gcgtcatgcc agctaacgaa gcaggctgtt
     5521 gtggcgcggt ggactatcat cttaatgcgc aggagaaagg gctggcgcgg gcgcgcaata
     5581 atattgatgc ctggtggccc gcgattgaag caggtgccga ggcaattttg caaaccgcca
     5641 gcggctgcgg tgcgtttgtc aaagagtacg ggcagatgct gaaaagcgat gcgttatatg
     5701 ccgataaagc gcgtcaggtc agtgaactgg cggtcgattt agtcgagctt ctgcgcgagg
     5761 aaccgctgga aaaactggca attcgcggcg ataaaaaact ggccttccac tgcccgtgta
     5821 ccctacaaca tgcgcaaaag ctgaacggcg aagtggaaaa agtgttgctt cgtcttggat
     5881 ttaccttaac ggacgttccc gacagccatc tgtgctgcgg ttcagcggga acatatgcgt
     5941 taacgcatcc cgatctggca cgccagctgc gggataacaa aatgaatgcg ctggaaagcg
     6001 gcaaaccgga aatgatcgtc accgccaaca ttggttgcca gacgcatctg gcgagcgccg
     6061 gtcgtacctc tgtgcgtcac tggattgaaa ttgtagaaca agcccttgaa aaggaataac
     6121 aaaatgaaaa ctaaagtcat tcttagccag caaatggcga gtgcaattat tgccgcaggt
     6181 caggaagagg cgcagaaaaa caactggtct gtttccattg ctgttgccga tgacggcggt
     6241 catctgctgg cgttaagtcg catggacgat tgcgcgccga ttgcggctta catctcccag
     6301 gagaaagcgc gtaccgccgc gctggggcgt cgtgaaacta agggctatga agagatggtg
     6361 aacaacggac gtaccgcgtt cgtgactgcg ccgttattaa cgtcgctgga aggcggcgta
     6421 ccggttgttg ttgatgggca aattattggt gccgtgggcg tttctggttt aaccggagca
     6481 caggatgcgc aggtcgcgaa agcggcagca gcggtgttgg cgaaataagc gaaaacgagg
     6541 agagaaacaa tgagtcaaac cataacccag ggccgtttac gcattgacgc caattttaaa
     6601 cgttttgtgg atgaagaagt tttaccggga acagggctgg acgctgcggc gttctggcgc
     6661 aattttgatg agatcgttca cgatctggca ccagaaaatc gtcagttgct ggcagaacgc
     6721 gatcgcattc aggcggcgct tgatgagtgg catcgcagca atccggggcc ggtaaaagat
     6781 aaagcggcct ataaatcttt cctgcgtgaa ctgggctacc tggtgccgca accggagcgc
     6841 gtgacggtgg aaaccacggg cattgacagc gaaatcacca gtcaggcggg gccacagctg
     6901 gtggttccgg caatgaacgc ccgctacgcg ctgaacgcgg cgaacgctcg ctggggctca
     6961 ctgtacgatg cgttatacgg cagcgacatc atcccgcagg aaggggcgat ggccagcggc
     7021 tacgatccgc aacgcggtga gcaggttatc gcctgggttc ggcgtttcct cgatgaatct
     7081 ctaccgctgg aaaacggcag ctatcaggat gtggtggcgt ttaaggtggt cgataaacaa
     7141 ttacgcatcc agttgaaaaa tggtaaagaa accacgttac gtaccccggc gcagtttgtc
     7201 ggttaccgtg gcgatgccgc tgcgccgacc tgcattttgc tgaaaaataa cggcctgcat
     7261 attgaactgc aaattgatgc caacgggcgg attggcaaag acgatccggc gcacatcaac
     7321 gatgttatcg tcgaagcggc catcagtacc attctcgact gcgaagattc ggtcgcggcg
     7381 gttgatgcgg aagataaaat cctgctgtac cgcaacctgc tgggcctgat gcaggggact
     7441 ctgcaagaga aaatggagaa aaacggtcga caaatcgtgc gtaaactgaa tgacgatcgt
     7501 cattacaccg ccgccgatgg ctctgaaatt tctctgcacg gacgctcgct gctgtttatc
     7561 cgcaacgtgg gtcatttgat gaccattcct gtgatttggg acagcgaagg caatgaaatc
     7621 ccggaaggca ttcttgatgg cgtcatgact ggcgcgattg ccctctatga tttaaaagtg
     7681 cagaaaaact cgcgcactgg cagcgtctat attgtgaaac cgaaaatgca cggtccgcag
     7741 gaagtggcgt tcgccaacaa actgtttacc cgcattgaga caatgctcgg tatggcaccg
     7801 aataccctga aaatgggcat tatggatgaa gaacgtcgga cctcgctgaa cttgcgtagc
     7861 tgtatcgctc aggcgcgcaa ccgcgtggcg ttcatcaata ccggtttcct cgaccgtacc
     7921 ggcgatgaaa tgcattcggt gatggaagct ggcccgatgc tgcgtaaaaa ccagatgaaa
     7981 tcgacgccgt ggatcaaagc ctacgaacgt aataacgtgc tttccggtct gttctgtggg
     8041 ctgcgcggta aagcgcaaat tggtaaaggc atgtgggcaa tgccggacct gatggcagac
     8101 atgtacagcc agaagggcga ccaactgcgt gccggggcaa acacagcctg ggttccgtca
     8161 ccaaccgctg ctacgctcca tgcgctgcac taccaccaaa ccaacgtaca gagcgtgcaa
     8221 gccaacattg cccagaccga gttcaatgct gaatttgaac cgctgctgga cgatctgctg
     8281 actattccgg ttgctgaaaa cgctaactgg tcggcgcaag agatccaaca agagctggat
     8341 aacaacgtgc aggggattct ggggtacgtg gtgcgctggg tggagcaggg gattggttgt
     8401 tcaaaagtgc cggatattca caatgtggcg ctgatggaag accgcgcaac gctgcgtatc
     8461 tccagccagc atatcgccaa ctggttacgt cacggtattc tgaccaaaga acaggtgcag
     8521 gcgtcgctgg agaatatggc gaaagtggtt gatcagcaaa acgctggcga tccggcttat
     8581 cgtccgatgg cggggaattt cgctaactcg tgtgctttta aagctgccag cgatttgatc
     8641 ttcctcggcg tgaaacagcc aaacggttat accgaaccgt tattacacgc ctggcgttta
     8701 cgcgaaaaag aaagtcatta atagcgtcaa aaaagccccg gcaagtttta ttgtcggggc
     8761 tatgatagta actgcggtag tacatttatc ccggaaataa ccggagcgat tatcagggtt
     8821 taacaatatt aaatacccgt agcgcattac tagcgggcct aaaacaacaa tatggacatc
     8881 ttaacgatac tctgcgcctg cgtgtgcgga acagaaaccc ttacccgata tacttgttat
     8941 gccagaggta gcaattgcgt tagcggcgta ctgttgctgt aaagtataac gcctatattt
     9001 tacagttcag tcctcttttg cttattacaa ccttaagtat atcaagcata taaagataat
     9061 aagagactga acaatatggt tacctggacc caaatgtata tgccgatggg aggactgggg
     9121 ctatccgctc tggtcgccct gatcccgata atattcttct tcgttgcact cgcggtatta
     9181 cgtctgaaag gacatgtcgc tggagcaata acccttatat tatctatcct gattgcaata
     9241 ttcgccttta aaatgccgat tgatatggca tttgctgctg cgggctatgg ctttatttat
     9301 ggattatggc caattgcatg gattattgtc gcggcggtat tcctctataa attaaccgtt
     9361 gccagcgggc agttcgatat tatccgcagc tcggttatct ccatcaccga cgatcagcgt
     9421 ttgcaggtgt tactgattgg tttctccttt ggtgcgttgc tggaaggagc ggctggcttt
     9481 ggtgcgccgg tggcgattac cggtgcgctg ctggtgggcc tgggcttcaa accgttatac
     9541 gcggcggggc tgtgtctgat tgccaatact gcgccggtgg cgtttggtgc gttgggcgtg
     9601 ccgattctgg tcgccggtca ggtaacggga atcgatccgt tccacattgg cgcaatggcg
     9661 ggacgtcagt taccgttcct gtcggttctt gtgccgttct ggctggtagc aatgatggac
     9721 ggctggaaag gggtgaaaga gacgtggcca gcggcgctgg ttgctggggg aagcttcgct
     9781 gtcactcagt tctttacctc taattatatt ggtccggaac tgccggatat tacttcggcg
     9841 ctggtgagta tcgtctcact cgctttattc cttaaagtct ggcggccgaa aaataccgaa
     9901 acggcaatca gcatgggaca atccgcaggt gcgatggtgg taaataagcc atcttctggc
     9961 ggtcccgtgc cttcagaata tagtctgggg caaatcattc gagcgtggtc accgttttta
    10021 atcttaacgg tgctggtcac catctggacc atgaagccgt ttaaagcgtt atttgctccg
    10081 ggcggcgcgt tttattcact ggtgattaat ttccagatcc ctcatttgca tcaacaagtg
    10141 ttgaaagcgg cacccattgt cgcccaacca acgccaatgg atgcggtgtt taaattcgac
    10201 cccctctcgg ctggcggcac cgctattttt attgcggcga ttatctctat cttcatcctc
    10261 ggtgtgggga tcaagaaagg tattggcgtc tttgccgaaa cgctaattag tctgaagtgg
    10321 ccgatactgt cgattggcat ggtgctggcg ttcgccttcg tcaccaacta ttctggcatg
    10381 tccaccacgc tggcgctggt actggcaggt acaggcgtga tgttcccgtt cttctcaccg
    10441 tttctcggct ggctgggcgt attccttacc ggctcggaca cctcctctaa cgccctgttt
    10501 ggttcactgc aatcgaccac ggcgcagcaa atcaacgtct ctgacactct gctggtggca
    10561 gcaaatacca gcggcggcgt aactggcaag atgatctccc cgcaatctat cgccgtggcc
    10621 tgcgccgcga cgggcatggt gggccgagaa tctgaactgt tccgctacac cgtgaagcac
    10681 agtctgattt ttgccagcgt tatcggcatt atcaccctgc tgcaggcgta tgtgtttacc
    10741 gggatgttag tctcgtaaat caataaagcc ggatgatcgt caatatcatc cggcttcat
//
LOCUS       DAZKFU020000159        10796 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZKFU020000159 DAZKFU020000000
VERSION     DAZKFU020000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.160"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..280
                     /gene="ytfI"
                     /locus_tag="ACHOQQ_RS16610"
                     /old_locus_tag="pgaptmp_003322"
     CDS             <1..280
                     /gene="ytfI"
                     /locus_tag="ACHOQQ_RS16610"
                     /old_locus_tag="pgaptmp_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HGU4471534.1"
                     /translation="LKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQV
                     DAIVSANLAADSDITKEKICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(343..897)
                     /locus_tag="ACHOQQ_RS16615"
                     /old_locus_tag="pgaptmp_003323"
     CDS             complement(343..897)
                     /locus_tag="ACHOQQ_RS16615"
                     /old_locus_tag="pgaptmp_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4471535.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1222..1428
                     /locus_tag="ACHOQQ_RS16620"
                     /old_locus_tag="pgaptmp_003324"
     CDS             1222..1428
                     /locus_tag="ACHOQQ_RS16620"
                     /old_locus_tag="pgaptmp_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGU4471536.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(1490..2833)
                     /locus_tag="ACHOQQ_RS16625"
                     /old_locus_tag="pgaptmp_003325"
     CDS             complement(1490..2833)
                     /locus_tag="ACHOQQ_RS16625"
                     /old_locus_tag="pgaptmp_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGU4471537.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3156..3794)
                     /gene="msrA"
                     /locus_tag="ACHOQQ_RS16630"
                     /old_locus_tag="pgaptmp_003326"
     CDS             complement(3156..3794)
                     /gene="msrA"
                     /locus_tag="ACHOQQ_RS16630"
                     /old_locus_tag="pgaptmp_003326"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGU4471538.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4000..5733
                     /gene="tamA"
                     /locus_tag="ACHOQQ_RS16635"
                     /old_locus_tag="pgaptmp_003327"
     CDS             4000..5733
                     /gene="tamA"
                     /locus_tag="ACHOQQ_RS16635"
                     /old_locus_tag="pgaptmp_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGU4471539.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            5730..9509
                     /gene="tamB"
                     /locus_tag="ACHOQQ_RS16640"
                     /old_locus_tag="pgaptmp_003328"
     CDS             5730..9509
                     /gene="tamB"
                     /locus_tag="ACHOQQ_RS16640"
                     /old_locus_tag="pgaptmp_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGU4471540.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRAALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMDVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDITKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLSAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            9512..9853
                     /locus_tag="ACHOQQ_RS16645"
                     /old_locus_tag="pgaptmp_003329"
     CDS             9512..9853
                     /locus_tag="ACHOQQ_RS16645"
                     /old_locus_tag="pgaptmp_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGU4471541.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(10136..10276)
                     /locus_tag="ACHOQQ_RS16650"
                     /old_locus_tag="pgaptmp_003330"
     CDS             complement(10136..10276)
                     /locus_tag="ACHOQQ_RS16650"
                     /old_locus_tag="pgaptmp_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4471542.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHSSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            complement(10409..10711)
                     /locus_tag="ACHOQQ_RS16655"
                     /old_locus_tag="pgaptmp_003331"
     CDS             complement(10409..10711)
                     /locus_tag="ACHOQQ_RS16655"
                     /old_locus_tag="pgaptmp_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4471543.1"
                     /translation="MFHCPFCGASARIRTSKAANDTGTIRYKYYQCNNLECSATFSTM
                     ESVHRIIGQPHLIFHDKDIPRETMRKSHQGRSQLTLQLEDCGISSKPVHYARVPFG"
BASE COUNT         2683 a         2635 c         2932 g         2546 t
ORIGIN      
        1 gcttaaaatc tatggagggg atgaacgttt tcgtaatggt tcctctgttg ttttggagga
       61 tgaagattac tctcttattt ataattttct ggttaatgca ggttgccaac aagtcgaatt
      121 agccgaacaa gtggatgcga tagtaagtgc gaatctggcc gcagatagtg atattaccaa
      181 agaaaagatt tgcgagaaat ataaatcgac cattgaggcg tttaaaaaag agcaactagc
      241 gttaccagta ttagttcggc gtaagaactc agagacttaa ttcccctatc ccatagataa
      301 cgatagggga aaattatttt tgcagaattt atgttattgc cattatttga gcaacttttg
      361 cagcaggtcc atcacttgct gcacctcttc ctgagtgagc gccccgtctt tggcccattg
      421 cacgcgacca tccttatcca gcaccactac agcggaactt tcttcatcca gctgccaggc
      481 accgcgcgcg acgccattgc tatcgacaat aaactgcgac cagggataaa gctttttatt
      541 actctccaga ctgctgcgca caaacatgcc ggaacccgga atcgcgtcgt cggtgttaac
      601 aatggtggtg gtctggtaac gatcgtgcgg taacttcgct gatttaatcg cttcaatcag
      661 cgtcgcgttt ttctcttttg cagaggtgcg accagcaata tgttgcagta ctcgcacttt
      721 tcccactaac tgcgcgctgt tccaggtttt gtagctaaac tgatctttat caagcaccaa
      781 ctcgccccga tcggtaatgc caatcggcgg cactcgctga ccggtttcga actgatgtgc
      841 ggaagccatc atcggcaaca gcaggcaggt gagtgccaga atcttgcgta gggtcatggt
      901 gtttccttct tatgatatgc aggtgatccg accacttggg tctgagtttt aatcatatgt
      961 gctatttatc gaatttcccg caagtgtgat gccagtttgc ggtcaagcgc acaaatcata
     1021 tgaaaaatga atgcttatac tgaagaccgc gcttcggtaa aaagataatt ctgaataatt
     1081 gtaaccttta ggtaaaaaaa gttatacgcg gtggaaacat tgcccggata gtctatagtc
     1141 actaagcatt aaaatttgcg cctcataata gttgggccga ttgtggcacc gcacaggcgt
     1201 aatactcagc aggagataac aatgaaaatt ttccaacggt acaacccact tcaggtggcg
     1261 aagtacgtaa agatcctgtt ccgtggacgg ttatacatca aggacgttgg cgcttttgaa
     1321 tttgataagg gtaagattct tatcccaaaa gtgaaggata agctgcattt gtcagtgatg
     1381 tccgaagtta accgtcaggt tatgcgtctg caaacagaga tggcttaacc aaagtgctat
     1441 gcagtaataa gacggctcct gattcaggag ccgttgatgt ttatgggggt tacgcgacgc
     1501 tttcttcttt atctttagcg tcaggcagtt ttggcgaaag ggctgtggcc ttgctgtcga
     1561 tccgggtcac cagcagctgg tcgatgcggt agttatcgat atccaccact tcaaatttgt
     1621 agccggcgaa tttcaccgaa tcggtgcgtt tcgggatctt acgcagcata aacatcataa
     1681 agccgccgat ggtttcgtag ttgcccgact gcgggaactc gtcaatatcc agcacgcgca
     1741 tgacgtcgtc aatcggagtg ccgccgtcaa tcagccatga gttctcatca cgggcgacaa
     1801 tctgctcttc cagtccctga ccgaccagat cgcccatcag cgtggtcatc acgtcgttga
     1861 gggtgatgat ccccactacc agcgcgtact cgttcatgat caccgcgaag tcttctcctg
     1921 cggttttaaa actttccaac gcctctgaaa gggttaacgt atccggcaca atcagcgtgt
     1981 tgcgaatttg tacgccgctg ttcagtgcca ggctttggtt agccagcacg cggttcagca
     2041 ggtctttaga atcgacatag ccgatgatgt ggtcaatatc ttcattacag acgaggaact
     2101 tagagtgcgg atgttccgcc accttatttt tcagactttg ctcatcttcg tggagatcaa
     2161 accaaatcac gttttcacgc ggtgtcattg aagacggaac ggtacgggat tccagctcaa
     2221 agacgttttc aatcagctcg tgttcctgtt tacgtaacac gcccgccagc gcaccggctt
     2281 ccactaccgc gtagatgtca tcagaagtga tgtcatcttt acgtaccatt ggcagtttga
     2341 aaatacggaa gattatgttc gccaggccgt tgaagaacca caccagcggg gtgcaaacgt
     2401 acaggcagaa gcgcatcggg ttgatgatac gcaaagccac agcttctggc gcaatcatac
     2461 cgatgcgttt cggggttaaa tccgcaaaca ggataaacat gccagtcact aacgagaaag
     2521 agagaataaa gctcagttgc tcagagagct ctgccgacat atagcgggag aacaggctgt
     2581 gaaaagctgg agaaaatgcc gcatcaccga cgataccgcc gagaatcgcc actgcgttca
     2641 gaccgatttg gaccacggta aagaacatgc cgggattttc ctgcatattc agaacgcgtt
     2701 gggcatttat attgccttca tcagccagca gtttaagttt gattttgcgt gaggcggcaa
     2761 gcgagatctc ggacatcgag aagaacgcac ttacagcgat caagcagagt atgactaaaa
     2821 tactgtttaa catatcttat ccgacctttc aggccagatc ctcggaaggg gaagttgatt
     2881 aatttgtgtg aaatacacat tgaaagccga ttcgaagaaa gtgaaccggc attttcagtg
     2941 gctagtatag cgtaaggtac tgtaaagtcg ccagagggtt aaaattcaga ctgctgataa
     3001 atgcaatact gcctgatgcg ctatgccgat caggcctacg cagtccttgc aatatattga
     3061 atttggacga ttttgtaggc ctggtaaggc gtttacgccg catccggcat aaataaagcg
     3121 cactttatca acaatctaca tttgtacccg taacgctatg cttccggtgg cagacagacg
     3181 ccaattccgc caattccaca gtaaccatac gggtttttat gcagatattg ctggtggtca
     3241 tcttcggcat aataaaacgg tgtggcgtta gcgatttccg tggtgatgtg acgatcgtca
     3301 tcggcggcaa gcatcgccgc ctgaaaacgt tccagactgg cgcgagctgc ggcatcctgt
     3361 tctggggtca gcggataaat cgctgaacga tactgcgtgc cgtggtcatt gccctgacgc
     3421 attccctggg cgggatcgtg attctcccaa aatacctgta gcaactgctc atagctgatg
     3481 acggaaggat cgtaaacaat gcgtaccgct tcggcatgac ccgtatcacc ggagcacact
     3541 tcccgataag tcggatttgg cgtgtagccg ccggtatagc ctgcggcggt gctgtaaacg
     3601 ccgggtaact gccagaacag acgctccaca ccccagaaac aacccatcgc aaaaatggca
     3661 atctccattc cgtcaggtac attggtcatt gagtgaccgt tgaccgcatg cagcgtggct
     3721 acgggcatcg gggtgttacg tccaggcagg gcatcggcgg gggaaaccag atgcttttta
     3781 tcaaataaac tcatggtgtc gctctcccga aaatcggtca ttggggttaa ggttgtaaca
     3841 agaggcgtat ttgcacacaa taaccaccgt gaataggtct aaagtaaaac ataagaaata
     3901 tttggggttt agtctgcttt ttaatccata ttactggatt tttgttaagc cgtttaacgg
     3961 cgttccaggg gcaggaaaaa aggatattca ggagaaaatg tgcgctatat ccgacagtta
     4021 tgctgtgtaa gcttactctg cttaagcgga tctgccgtcg ccgcgaacgt ccgtctacag
     4081 gtcgaggggt tatcgggaca gctggaaaag aacgttcgcg cgcagctttc tacgattgaa
     4141 agtgatgaag tgacgccaga ccgtcgcttt cgcgcacgcg tcgatgatgc tatccgcgaa
     4201 ggtctgaaag cgctgggtta ttaccagccg accattgaat ttgatctccg tccaccgcca
     4261 aagaaagggc ggcaggtatt gatcgccaaa gtcacgccag gcgtgccggt gttaattggc
     4321 ggcaccgatg tggtattgcg cggcggcgcg cggaccgata aagactattt gaaattgctc
     4381 gatactcgcc cggctattgg tacggtactg aaccagggcg attatgaaaa tttcaaaaag
     4441 tccttaacca gcattgcgtt gcgtaaaggt tatttcgata gcgaatttac caaagcgcag
     4501 ctgggcattg cgctcggcct gcataaagcc ttctgggata ttgattataa cagtggcgaa
     4561 cgttaccgct ttgggcatgt gacctttgaa ggatcacaaa tccgcgatga atacctgcaa
     4621 aatctggtgc cgtttaaaga gggcgatgag tacgaatcga aagatctggc agaactgaac
     4681 cgccgacttt ctgctaccgg ctggtttaac tcggtggtgg tggctccaca atttgataaa
     4741 gcgcgcgaaa cgaaagtatt accattgacg ggcgtggttt cgccgcgaac agaaaacacc
     4801 atcgaaaccg gggtcggtta ctctacggac gtgggaccgc gcgtgaaagc gacgtggaaa
     4861 aagccgtgga tgaactctta tggtcacagt ctgaccacca gtaccagtat ttccgcgccg
     4921 gaacagaccc tcgacttcag ctataaaatg ccgctgctga agaatccact ggaacaatat
     4981 tatttggtgc agggcggttt taagcgcact gacctgaacg ataccgagtc tgactccact
     5041 acgctggtgg cttctcgcta ctgggatctc tccagcggct ggcagcgtgc cattaacctg
     5101 cgctggagtc tcgaccactt tactcagggt gaaattacca acaccacgat gctgttttat
     5161 cctggggtga tgattagccg cacgcgttct cgtggtggcc tgatgccaac ctggggcgac
     5221 tcgcaacgct actctatcga ctactccaac actgcctggg gctcagatgt cgatttctcc
     5281 gttttccagg cacaaaacgt ctggatccgc acactgtacg atcgccatcg ttttgtgaca
     5341 cgcggcacgc tgggctggat tgaaaccggt gatttcgaca aagtaccgcc ggatctgcgt
     5401 tttttcgccg ggggcgaccg cagtattcgt ggctacaaat acaaatctat cgctccgaaa
     5461 tacgccaacg gtgacctgaa aggggcctcg aagttgataa ccggatcgct ggagtaccag
     5521 tacaacgtga ccggaaaatg gtggggcgcg gtgtttgtcg atagtggcga agcggtaagc
     5581 gatattcgcc gcagcgactt taaaaccggt accggggtcg gcgtacgctg ggaatcgccg
     5641 gtcgggccaa tcaaactcga ttttgccgta ccggtcgcgg ataaagacga acacgggtta
     5701 cagttttaca tcggtctggg gccagaatta tgagtttatg gaaaaaaatc agcctcggcg
     5761 tggttatcgt tatcttactg ttgctgggat cggtggcgtt tctggtgggc accaccagcg
     5821 gcctgcatct ggtatttaaa gcggcggatc gctgggtgcc aggactggat attggcaagg
     5881 tcaccggcgg ctggcgcgat ctcaccttgt ctgacgttcg ttatgagcag ccaggcgtgg
     5941 cggtaaaagc aggcaatctg catctggcgg tcgggcttga gtgcctgtgg aacagcagtg
     6001 tttgtattaa tgatctggcg ctgaaagaca tccaggtcaa tatcgacagc aaaaaaatgc
     6061 ccccttctga acaggttgaa gaagaggaag atagcggtcc gctggatctc tccacgccgt
     6121 atcccatcac cctgacacgg gcggcactgg acaacgtcaa catcaagatt gatgacacca
     6181 cggtatcggt gatggacttc acctccggcc tgaactggca ggagaaaacc ctgaccctga
     6241 aaccgacgtc gctgaaaggc ctgctgattg ctctgccgaa agtggcggaa gtggcgcagg
     6301 aagaagtggt cgaaccgaaa attgaaaatc cgcagccgga ggaaaagccg ctcggcgaaa
     6361 cgctgaaaga tctcttttct cgcccggtat tgccggaaat gaccgacgtg catttgccgc
     6421 ttaacctgaa cattgaagag tttaagggcg agcagttgcg cgtgacgggc gacacggaca
     6481 tcaccgtgcg caccatgctg ctgaaagtga gcagcattga cggcaatact aaactggacg
     6541 ccctggatat cgattccaac caggggatcg tcaacgccag cggcacggcg cagctgtcag
     6601 acaactggcc ggtggacatc actctcaaca gtacactgaa cgtggagccg ttgaaaggtg
     6661 aaaaagtgaa gctgaaagtg ggcggcgcgc tgcgcgaaca gctggagatt ggcgttaatc
     6721 tttccggtcc ggtggatatg gatttacgcg cccagacgcg actggcggaa gccggattgc
     6781 cgctcaacgt ggaagtgaac agcaaacaga tttactggcc gttcactggt gagaagcagt
     6841 atcaggcgga tgatctgaaa ctgaaactta ccggcaaaat gaccgattac acgctctcta
     6901 tgcgtacggc agtgaaggga caggagatcc cgcccgcgac tattaccctc gacgccaaag
     6961 gtaatgaaca gcaggtcaat ctcgacaaac tcaccgtcgc ggcgctggaa gggaaaactg
     7021 aactcaaggc gttgctcgac tggcagcagg ccattagttg gcgcggtgag ctaacgctta
     7081 atggcattaa caccgccaaa gagttcccag agtggccgtc gaaactcaat gggttgatta
     7141 aaacccgcgg tagcctgtac ggcggcacct ggcagatgga cgtgccggag ttaaagctga
     7201 ccggtaacgt taaacagaac aaagtgaacg ttgacggcac gctgaaaggc aacagttata
     7261 tgcagtggat gatcccaggg cttcatctgg aactcgggcc aaacagtgcc gaagtgaaag
     7321 gcgagctggg ggtaaaagat ctcaatcttg atgccaccat caacgcgccg gggctggata
     7381 acgcgctgcc ggggcttggc ggtacagcga aagggctggt gaaagtacgc ggcacggtgg
     7441 aagcgccaca actactggca gatatcaccg cgcgcggcct gcgctggcag gaactttccg
     7501 tggcgcaggt tcgcgtggaa ggcgacatta aatccaccga tcagatcgcc gggaaactcg
     7561 acgtacgcgt tgagcaaatt tcgcagccgg atgtaaatat caacctcgtc accctgaatg
     7621 ccaaaggcag cgaaaagcag cacgagctac agttgcggat tcagggcgag cctgtctccg
     7681 ggcagcttaa tctggcagga agttttgatc gcaaagaaga acgctggaag ggaacactta
     7741 gcaatacccg cttccagacg ccggtcggcc cgtggtcgct gacccgcgat attgcgctgg
     7801 attaccgcaa taaggagcaa aaaatcagca tcgggccaca ctgctggctt aacccgaatg
     7861 cggaactgtg cgtgccgcaa actatcgatg cgggggccga agggcgtgcg gtggtgaatc
     7921 tcaaccgctt cgacctcgcc atgctgaaac cgtttatgcc agaaaccact caggccagcg
     7981 gtatcttcac gggtaaagcg gacgttgcct gggacatcac gaaagagggg ctgccgcagg
     8041 gcagtatcac tctttcgggg cgtaacgttc aggtaacgca aaccgtcaac gatgcggcgc
     8101 tgcccgtggc gtttcagacg ttgaatctgt cggcggagtt gcgtaacaac cgtgccgaat
     8161 tgggctggac catccgcctg accaataacg gccagtttga tggacaggtg caggtgaccg
     8221 atccgcaagg ccgccgtaat cttggtggca acgtcaatat ccgtaacttc aaccttgcga
     8281 tgataaaccc catctttacc cgtggggaaa aagcagcggg gatggtgagt gccaacttgc
     8341 gtctgggtgg tgatgtgcaa agcccgcagt tgtttggtca gcttcaggtt acgggtgtgg
     8401 atatcgatgg caactttatg ccgtttgata tgcaaccgag ccagcttgcg gtcaacttta
     8461 acggtatgcg ctcgacgctt gccggtacag tacggaccca gcagggtgaa atctacctga
     8521 acggtgatgc cgactggagc caaattgaaa actggcgggc gcgggtaacg gcgaaaggca
     8581 gtaaagtgcg gatcaccgtg ccgccgatgg tacgaatgga tgtatcgcca gatgttgtat
     8641 tcgaggctac accaaacctg tttaccctcg atggtcgcgt ggatgtcccg tgggcgcgca
     8701 tcgtggtgca cgatctgccg gaaagcgcag taggcgtctc cagcgatgtg gtgatgctta
     8761 acgataacct gcaaccggaa gagccgaaaa cggcgtcgat tccgattaac agtaacctga
     8821 ttgtccacgt tggcaacaat gtgcgcattg acgcctttgg cctgaaagcg cggctgacgg
     8881 gcgatcttaa cgtcgttcag gacaaacaag ggctgggcct gaacgggcag atcaacatcc
     8941 ctgaagggcg cttccatgcc tatggtcagg atctgattgt gcgtaaaggt gagttactgt
     9001 tctctggccc gccggatcaa ccgtatctta atattgaagc tattcgtaac ccggatgcta
     9061 cagaagacga cgtaatcgcc ggagttcgcg tcactggtct ggcggacgaa ccgaaagcgg
     9121 agatcttctc tgacccggcg atgtcgcaac aagctgcctt gtcttatttg ctacgtggac
     9181 aagggctgga gagcgatcag agcgacagtg cggcaatgac ctcgatgctg attggtctgg
     9241 gggttgcgca aagtggccag attgtgggta aaatcggcga gacgtttggc gtaagcaatt
     9301 tagcgctcga cacccaggga gtaggcgact cctcccaggt agtggtcagc ggctatgtat
     9361 tgccaggtct gcaagtgaaa tacggcgtgg gtatatttga ctctatagca acactcacgt
     9421 tacgttatcg cctgatgcct aagctatatc tggaagccgt gtctggtgta gaccaggcac
     9481 tggatttgct ctatcagttc gagttttagc aatgcgaata tttgtctacg gcagtttacg
     9541 ccacaaacaa ggcaacagtc actggatgac caatgcccag ttactgggcg atttcagtat
     9601 cgataactac cagttgtata gcctgggcca ctatccaggc gcagttccgg ggaacggaac
     9661 ggtacacggt gaagtttatc gtattgacaa cgccacgctg gccgaacttg atgccttgcg
     9721 caccaggggc ggtgaatacg cgcgccagtt gattcagacg ccgtacggga gtgcatggat
     9781 gtacgtttat caacgacccg tcgatggatt aaagctaatt gaaagcggcg actggttaga
     9841 cagggataag taaccctatg catacgccac cttcgggtgg cgcgaaaatt gatgtaatac
     9901 aatgaaaaat aaaggattat ttttcgagtt gtccacatgt tgccatggcg ttctaaattt
     9961 ttttggtgag actatctctg cttgttgttt tcaccgccag cttgttaaac aaaaaaagcc
    10021 cacagttttt acgctgcggg ctttgttttt ctttgtgact tgcgtgtgag ggtgcgactt
    10081 tcgaccgaac cttcggcaac cggatagcgg gggcgttcac ccccgctgcg gtttcttacg
    10141 gtttacactg caagaacgcc gcaaattctg accacatgat gttaagcctg aactcacacg
    10201 gcgaactgtg tatcatccag acagtcaaca acatggttaa gcataaaatg ctcacgatga
    10261 tcgttttttg cggcatagcg cttgctcctt tttcaaagag gcgctaacct tccacttgtc
    10321 taaggtagga tggttagggc ctcgggttaa acaatatgtt tgactcgggg cctttccaca
    10381 tccggccttc aggttttcct tccagccgtc agccgaaagg cacccgcgcg taatgtactg
    10441 gttttgagga tatgccgcaa tcctccagtt gtagcgttaa ctgactgcgt ccctgatggg
    10501 atttgcgcat tgtctcccgt ggaatatctt tatcgtggaa gatcaaatga ggctggccaa
    10561 taattctgtg cacactttcc attgttgaaa atgtggcgct gcactcaagg ttattgcact
    10621 gatagtattt gtagcggatg gtgccggtat cattggcggc tttactggta cggatccggg
    10681 cagatgcgcc gcagaatgga caatgaaaca ttgatatacc ccctggctga actggggttg
    10741 aactcgtcgc cattctaacc attgctttcc ttttcggcta tccactcggg tattct
//
LOCUS       DAZKFU020000160        10739 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZKFU020000160 DAZKFU020000000
VERSION     DAZKFU020000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.161"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            217..1584
                     /gene="dcuC"
                     /locus_tag="ACHOQQ_RS16660"
                     /old_locus_tag="pgaptmp_003332"
     CDS             217..1584
                     /gene="dcuC"
                     /locus_tag="ACHOQQ_RS16660"
                     /old_locus_tag="pgaptmp_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4471544.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHKLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1626..2123)
                     /gene="sspB"
                     /locus_tag="ACHOQQ_RS16665"
                     /old_locus_tag="pgaptmp_003333"
     CDS             complement(1626..2123)
                     /gene="sspB"
                     /locus_tag="ACHOQQ_RS16665"
                     /old_locus_tag="pgaptmp_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGU4471545.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2129..2767)
                     /gene="sspA"
                     /locus_tag="ACHOQQ_RS16670"
                     /old_locus_tag="pgaptmp_003334"
     CDS             complement(2129..2767)
                     /gene="sspA"
                     /locus_tag="ACHOQQ_RS16670"
                     /old_locus_tag="pgaptmp_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGU4471546.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3162..3554)
                     /gene="rpsI"
                     /locus_tag="ACHOQQ_RS16675"
                     /old_locus_tag="pgaptmp_003335"
     CDS             complement(3162..3554)
                     /gene="rpsI"
                     /locus_tag="ACHOQQ_RS16675"
                     /old_locus_tag="pgaptmp_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGU4471547.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3570..3998)
                     /gene="rplM"
                     /locus_tag="ACHOQQ_RS16680"
                     /old_locus_tag="pgaptmp_003336"
     CDS             complement(3570..3998)
                     /gene="rplM"
                     /locus_tag="ACHOQQ_RS16680"
                     /old_locus_tag="pgaptmp_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGU4471548.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4217..5344)
                     /gene="zapE"
                     /locus_tag="ACHOQQ_RS16685"
                     /old_locus_tag="pgaptmp_003337"
     CDS             complement(4217..5344)
                     /gene="zapE"
                     /locus_tag="ACHOQQ_RS16685"
                     /old_locus_tag="pgaptmp_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGU4471549.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDEIRAQMDKLWLALAGAKREISPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHAVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5538..5936
                     /gene="zapG"
                     /locus_tag="ACHOQQ_RS16690"
                     /old_locus_tag="pgaptmp_003338"
     CDS             5538..5936
                     /gene="zapG"
                     /locus_tag="ACHOQQ_RS16690"
                     /old_locus_tag="pgaptmp_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGU4471550.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6090..7457
                     /gene="degQ"
                     /locus_tag="ACHOQQ_RS16695"
                     /old_locus_tag="pgaptmp_003339"
     CDS             6090..7457
                     /gene="degQ"
                     /locus_tag="ACHOQQ_RS16695"
                     /old_locus_tag="pgaptmp_003339"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGU4471551.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7547..8614
                     /gene="degS"
                     /locus_tag="ACHOQQ_RS16700"
                     /old_locus_tag="pgaptmp_003340"
     CDS             7547..8614
                     /gene="degS"
                     /locus_tag="ACHOQQ_RS16700"
                     /old_locus_tag="pgaptmp_003340"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGU4471552.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8677..9615)
                     /gene="mdh"
                     /locus_tag="ACHOQQ_RS16705"
                     /old_locus_tag="pgaptmp_003341"
     CDS             complement(8677..9615)
                     /gene="mdh"
                     /locus_tag="ACHOQQ_RS16705"
                     /old_locus_tag="pgaptmp_003341"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4471553.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            10050..10520
                     /gene="argR"
                     /locus_tag="ACHOQQ_RS16710"
                     /old_locus_tag="pgaptmp_003342"
     CDS             10050..10520
                     /gene="argR"
                     /locus_tag="ACHOQQ_RS16710"
                     /old_locus_tag="pgaptmp_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGU4471554.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2671 a         2717 c         2652 g         2699 t
ORIGIN      
        1 tgaattattt acaagactat tcacgagcat tatgaatatt atgaatgtgt tcttacaaaa
       61 taatcataag cgcatatttt ttaatgaaaa atcacctcac ctacaattaa aaacacgaca
      121 tcagcaccat aaatagactt gcaaaaaata taacatcatt gttttcaatc tgccatttat
      181 gggattgacc gttttctttt gacacggagt tcaacaatgt tcggcataat tatatctgtc
      241 atcgtattaa ttacgatggg ctatttgatc ctgaaaaact acaaacctca ggtggtgctg
      301 gctgccgcag gtatcttcct gatgatgtgc ggtgtctggt tagggttcgg tggtgtactc
      361 gatcccacca aaagcagcgg ctacttgatc gtcgatattt ataatgaaat cctgcgcatg
      421 ctgtccaacc gcattgccgg attggggctg tcgattatgg cggtgggcgg ttatgcccgc
      481 tacatggagc gcataggggc cagtcgcgcg atggtgagct tgttaagccg cccgttaaaa
      541 ctcattcgct cgccgtatat tattctgtcg gcaacttacg tcatcggcca aatcatggcg
      601 cagtttatta ccagcgcctc cggtctgggt atgttgctga tggtcacctt atttccgacg
      661 ctggtgagtc tgggagtaag tcgcctctct gcggtggcgg ttatcgcaac cacgatgtcc
      721 attgagtggg ggattctgga aacgaactcc atttttgcag cccaggtcgc gggaatgaaa
      781 attgccactt acttcttcca ctaccagctt ccggtcgcct cttgcgtcat tatctcggtg
      841 gcgatctccc actttttcgt gcaacgcgct tttgacaaaa aagataaaaa tatcaatcac
      901 gaacaggcag agcaaaaaac tctcgataat gtcccgccgc tctattacgc cattttacct
      961 gtgatgccgt taatcctgat gctcggctcg ctattccttg cccacatcgg gctgatgcag
     1021 tcagaactgc atctggtggt ggtgatgtta ctgagtttga cggtgacgat gtttgttgag
     1081 ttcttccgca agcataaatt acgcgaaaca atggacgatg tgcaggcgtt ttttgacggc
     1141 atgggtacgc agtttgccaa cgtggtaacg ctggtggtcg cgggtgaaat atttgcgaaa
     1201 ggcttaacga cgattggcac tgtcgatgcg gttatcaggg gtgcggagca ttctggtctg
     1261 ggcggtattg gcgtgatgat tattatggcg ctggtcattg ccatttgtgc cattgtgatg
     1321 ggctctggca atgcgccgtt tatgtcattt gccagtctta ttccgaatat cgcagccgga
     1381 ctacatgtac cagcggttgt aatgattatg ccgatgcatt ttgccacgac gctagcgcgc
     1441 gcggtttcgc cgattactgc ggtggtagtc gttacgtcag gaattgcagg cgtttcgcct
     1501 tttgcggtgg tgaagcggac agcgatcccc atggcagtcg gtttcgtggt gaatatgatt
     1561 gccacaatca cgctatttta ttaagccatt aaaagacaaa acaggccacc agggcctgtt
     1621 ttgtattact taacaacgcg taatgccggt cgaccaccgc gtggtggctg cggaggttca
     1681 tcgtcaggat gagtgtcatc atcgtgatct ggcttgtcgc catcaataac cgacataacg
     1741 gtttcgttgt ctgccgatgc ctcttcatca ttcatgatgc tggcatcttc atcgtaggca
     1801 gcttcaggtt caaacatcgt gccagcgcca ttttcacggg cgtagatagc cagcacggca
     1861 gccagcggca cagaaacctg acgcggaatg ccaccaaagc gcgcgttaaa gcgcacctca
     1921 tcattcgcca gttccagatt gccgacagca cgcggcgcaa tgttgagtac gatttgcccg
     1981 tcacgcgcat attccatagg aacctgcacg ccagggagcg tcacatccac caccaggtgc
     2041 ggcgtgagct ggttatccag caaccactca tagaatgcac gcagcagata gggacgacgt
     2101 ggtgttagct gtgacaaatc catacagatt aactccggcc cagacgcatt tcacgttctg
     2161 cttcagttaa agaagcaagg aaagagtcac gctcaaagac gcgggtcata tagcctttca
     2221 gctctttcgc acccgggccg ctgaactcga tgcccagttg cggcagacgc cacagcagcg
     2281 gagcaaggta gcaatcgacc aggctgaact catcactcag gaagtacggc ttctgaccga
     2341 agaccggcgc aatcgccagc agttcttcgc gcagttgctt acgtgcggca tctgcttcag
     2401 aagctgaacc gttgatgatg gtgttcatca gcgtgtacca gtctttttcg atgcgatgca
     2461 tgtacagacg gctttcaccg cgagctaccg ggtaaacagg catcagtggc ggatgcggga
     2521 aacgctcatc cagatattcc ataatgatgc gagattccca cagggtcagc tcacgatcca
     2581 ccagggtcgg aacgctctga ttcgggttga ggtcaatcag atcctgaggc ggattgtcct
     2641 tttccacgtg ttcgatctcg aaacttacac ctttctcagc cagcacaatg cggacctgat
     2701 ggctatagat gtcagtagga ccggaaaaca gcgtcattac cgaacgtttg ttggcagcga
     2761 cagccatgaa aacctccagg tatagtcaga atttttactg ctaccagcca ccaggtggcc
     2821 agtcagaagt tgtgttaccc agtaaggaac gactctcttt gttcgaaaat caaacaaaaa
     2881 atgagcaata cccgacattt gggcagaaaa ttggatgata gtttaccaga ttttgcgacc
     2941 tttgtggtga gtcgatgccg gaaatgggga aaaagagatg cgctttagtc tgaaatagtt
     3001 gacttagccc cttattggcg atgtggtttt tgttttgcct gtctgtcaga cggcagcaaa
     3061 aagcaacttt ccatttttta cgctaattca aaatttagcc ataaaaaaac ccgccgaagc
     3121 gggttttttc gcaaattgtt ttctgccgga gcagaagcca attaacgttt ggagaactgc
     3181 ggacgacgac gtgctttacg cagaccgact ttcttacgtt caacctgacg agcgtcacga
     3241 gtaacgaagc cagctttacg cagttcagaa cgcagggact cgtcgtattc catcagagcg
     3301 cgggtgatac cgtgacggat cgcaccagcc tgaccagaga taccaccacc tttaacggtg
     3361 atgtacaggt ccagtttctc aaccatgtcg accagttcca gcggctgacg aactaccatg
     3421 cgggcagttt cacgaccgaa gtactgttcc agagaacgtt ggttgattac gattttaccg
     3481 ttgcccggtt tgatgaaaac gcgagctgcg gaacttttgc ggcgaccagt gccgtagtat
     3541 tgattttcag ccattgccta taatcccgat tagatgtcaa gaacttgcgg ttgctgtgcc
     3601 gcgtggttgt gctcgttacc cgcgtaaact ttcagtttac ggaacatagc acgacccagc
     3661 gggccttttg gcaacatgcc tttaaccgcg atttcaatca cacgctcagg acggcgagca
     3721 atcatctctt caaaggtcgc ttgtttgata ccaccgatgt ggccggtgtg gtgatagtac
     3781 actttgtcag tacgcttgtt gccggttaca gcaactttgt cagcgttcag aacgatgatg
     3841 taatcaccgg tatctacgtg cggagtgtat tccgctttgt gcttaccgcg caggcgacga
     3901 gccagttcag tagccagacg gcccagagtt ttaccggtcg cgtcaacaac ataccagtcg
     3961 cgttttacgg tttctggttt agctgtaaaa gttttcatta aaagcttacc caataaatag
     4021 ttacacgttg gtgaacaccc aaacgtcttc aattgttgag gttcacacga caaagtccgg
     4081 caaacctacc ccttcgaata gcctatgcca gcacacaaaa agttttggga aaaaaacttt
     4141 cttgtaacgt ggggtcgcag gattatagag aagtcggggt caaagatcga cccctttttg
     4201 tgatttgtgg caggttttaa cccgccaaat gctcgcgctt cagatactct tcgctttgca
     4261 tctcttgcag acgtgacagg caacgctgga actcaaactt cagccgctca ccctgataaa
     4321 tttcatacag cggcacttct gcactcacca ctaatttgac atggcgctcg taaaactcat
     4381 ccaccagcgc aataaagcgc cgcgcttcgc tctccatcaa ccgcgtcata actggtacat
     4441 caaacaacat gaccgcatga aagagacgtg agagcgcaat atagtcatgc tgactgcggg
     4501 cgtcgacgca cagcgtagta aaagagaccg ccagcgtctg gttctcgacg cccattgtcg
     4561 ctaatggccg atggttgatt tctaacgttg gtgaaatttc tcgtttcgcc cccgccagcg
     4621 ccaaccatag tttatccatt tgcgcccgga tttcatcgtt gagtggcgaa agccacagat
     4681 gcgcctgagt gagtgtacgc agtcgataat caacaccagc gtccacgttc attacatcac
     4741 aatgctgttt aatggcatcg attgcaggca gaaaacgcgc acgttgcagg ccatttcgat
     4801 aaagttcatc cggcggaata tttgacgtcg ctaccagggt aataccgcgg gcgaacaggg
     4861 ctttcatcag accgccaagt agcatggcgt cggtaatatc agaaacaaaa aattcgtcaa
     4921 aacagagcac gtcagtttcg gctttaaagc gatcggcaat aatttccagc ggatcggtct
     4981 gcccctgtaa ggcagttagc tcttcgtgca cccgcagcat aaaacggtga aagtgcaggc
     5041 gctgtttccg ctctcccggc aggctttgat agaaaaggtc catcagccag gttttcccgc
     5101 gtcctacacc gccccacata tataagccac gcactggcgt atgctttgtg tcttcgcgtt
     5161 taccccacag cttaccgacc cgcgccatta gcccactcgt cctgggggct ggtggcctgc
     5221 tattgatgag ttcctgataa ataatttcca ggcggctgac ggcctctttt tgaacgtcgt
     5281 cgggttgatg gctgccttca ttgagcgcct tcaggtattg cgatgttggg gtaacgcttt
     5341 gcatgatctt attgttattc cttgaataat cggtgcgccg ttgttcacgg ttgacgaaaa
     5401 aaaggccgtt ctacactacg cgatatgcag tcgggattcc acttctgtgg cattaacggt
     5461 tatagtggca taatcagccg caggcatgga gcctgaagcc aacaccctac ggaaacaaaa
     5521 gacaacggga gatgttcatg acctgggaat atgcgctaat tgggttagtc gtcggcatca
     5581 ttattggtgc tgtggccatg cgttttggta atcgtaaact acgccagcaa caggcgttgc
     5641 agtacgaact ggaaaagaat aaagctgaac tggacgagta tcgcgaagag ctggttagcc
     5701 actttgcccg cagcgcggaa ttactggata ccatggcgca cgactatcgc cagctgtatc
     5761 agcacatggc gaaaagctcc agcagcctgc tgccggaact gtctgctgaa gcaaacccgt
     5821 tccgtaatcg tctggcagag tctgaagcca gcaacgatca ggcaccggtg cagatgcctc
     5881 gcgactattc tgaaggcgca tccggcctgc tgcgtactgg cgcgaagcgc gactaattta
     5941 tttttcgggc gcagccattg cgccctcctc ttctctccct ccccgactat catttaatct
     6001 ggtgtctcat tgttagccgt ctgaaaattc aataacatca aactgttttg aatctctttt
     6061 cttatcattc aggtacgaga gcaggaataa tgaaaaaaca aacccagctg ttgagtgcat
     6121 tagcgttaag tgtcgggtta actctctcgg cgtcatttca ggccgtcgcg tcgattccag
     6181 gccaggttgc cgatcaggcc cccctcccca gtctggctcc aatgctggaa aaagtgcttc
     6241 cggcagtggt gagcgtacgg gtggaaggaa cggccagtca gggacagaaa atcccggaag
     6301 aattcaaaaa gttttttggt gatgatttac cggatcaacc tgcacaaccc ttcgaaggtt
     6361 taggctccgg tgtcatcatc aacgccagta aaggctatgt gctgaccaac aaccatgtga
     6421 ttaatcaggc acagaaaatc agtattcagc tcaatgatgg gcgcgagttt gatgcaaaac
     6481 tgattggtag cgatgaccag agcgatatcg ccctgttgca aattcaaaac ccgagcaaat
     6541 taacgcaaat cgctattgcc gactccgata aattgcgcgt cggtgatttt gccgtggcgg
     6601 tcggtaaccc atttggcctt gggcaaaccg ccacctctgg cattgtttcc gcattaggac
     6661 gcagcgggtt gaatcttgaa ggtctggaaa actttatcca gacagatgct tccattaacc
     6721 gcggtaactc cggcggtgca ctgttaaacc ttaacggtga gttaattggc atcaacactg
     6781 caatccttgc gcctggcggc gggagcgtcg gaattggatt tgccatcccc agtaatatgg
     6841 cgcgaacact ggcgcagcag cttatcgact ttggtgaaat caaacgcggt ttgttaggca
     6901 tcaaaggcac cgagatgagt gccgatatcg ccaaagcctt caaccttgac gtgcagcgtg
     6961 gcgcgtttgt cagcgaagtg ttgccaggtt ctggctcggc gaaagcgggc gtcaaagcgg
     7021 gcgatattat taccagcctc aacggcaaac cgctgaatag ctttgctgag ttgcgctctc
     7081 gtatcgcgac caccgagccg ggcacgaaag tgaagcttgg cctgctgcgt aacggcaaac
     7141 cactggaagt agaagtgacg ctcgatacca gcacctcttc gtcggccagc gctgaaatga
     7201 tcacgccagc gctggaaggt gcaacgttga gcgatggtca gctaaaagat ggcggcaaag
     7261 gtattaaaat cgatgaagtt gtcaaaggaa gcccagctgc tcaggctggc ttgcaaaaag
     7321 acgatgtgat cattggcgtc aaccgcgatc gggtgaactc gattgctgaa atgcgtaaag
     7381 tgctggcggc aaaaccggcc atcatcgccc tgcaaattgt acgcggcaat gaaagcatct
     7441 atctgctgat gcgttaatgt cgtaaaccgg gcatcaggct gacgtgtgat gtccggttaa
     7501 ctcgtggtat gctgctgccg ttcccttttt taatgacgcc tccatcatgt ttgtgaagct
     7561 cttacgttcc gttgcgattg gattaattgt cggcgctatt ctgctggttg ccatgccttc
     7621 gctgcgcagc cttaacccgc tttccactcc gcaatttgac agtaccgatg agacgcctgc
     7681 cagttataat ctggcggttc gccgcgccgc gccagcggtg gttaacgttt acaaccgtgg
     7741 tttgaacacc aactctcaca accagcttga gatccgcacc ctgggatccg gtgtaatcat
     7801 ggatcaacgc ggttatatca tcaccaataa acacgtcatc aacgacgccg atcagatcat
     7861 cgtcgcctta caggatggac gtgtatttga agcattgctg gtgggatctg actctctaac
     7921 cgatctggcg gtacttaaaa ttaatgccac tggcggttta cctaccattc cgatcaatgc
     7981 acgtcgcgta ccgcacattg gcgacgtggt actggcgatc ggtaacccgt acaacctcgg
     8041 gcagaccatt acccagggga ttattagtgc cacgggtcga atcggtctga acccgaccgg
     8101 gcggcaaaac ttcctacaaa ccgatgcttc cattaaccac ggtaactctg gcggcgcgct
     8161 ggtgaactcg ctgggagaac tgatgggcat taacacgctg tcgtttgata agagtaacga
     8221 tggcgaaacg ccggaaggta tcggctttgc gattcctttc cagttagcaa ccaaaattat
     8281 ggataagctg atccgcgatg gtcgcgtgat ccgcggctac attggtatcg gcggacgcga
     8341 gatcgcacca ctgcacgcgc agggcggtgg tatagatcaa ctgcaaggga tcgtggttaa
     8401 tgaagtgtca cctgacggcc cggcggcgaa tgcgggtatt caggtcaacg atctgattat
     8461 ttcggtggat aacaaaccgg ccatctctgc tctggagacg atggatcagg tggcagaaat
     8521 tcgccctggc tcggtgatcc cggttgtagt gatgcgtgat gataagcagt taacgctgca
     8581 ggtcaccatt caggaatatc cggcaaccaa ttaagtcgag tgctcaaaac aaaaaaccgg
     8641 agtctgtgct ccggtttttt attattcgct aatcaattac ttattaacga actcttcgcc
     8701 cagggcgata tctttcttga gcgtatccag cataccttcc agcgcgttct gttcaaatgc
     8761 gctcagggta ccgatagatt tacgctcttc cacgccgttt ttacccagca gcagcggttg
     8821 agagaagaaa cgggcgtact gaccgtcgcc ttcaacgtag gcacattcga caacgccttg
     8881 ttcgccctgc agtgcgcgaa ccagagacag accaaaacgt gcagctgcct ggcccataga
     8941 cagggttgca gacccgccac cggctttcgc ttcaaccacc tcagtacccg cgttctggat
     9001 acgtttggtc agatcagcca cttcctgctc ggtaaaacta acgccaggaa cctgtgacag
     9061 cagcggcaga atggtaacac cagagtgacc gccaataacc ggcacttcaa cttcgcctgg
     9121 ctgtttgcct ttcagttccg caacaaaggt gttggaacga atgatatcca gcgtggtaac
     9181 gccaaacagt ttgtttttgt cataaacacc ggcttttttc aacacttcag cagcaatcgc
     9241 aactgtggtg ttaaccgggt tagtgataat accaatgcac gctttcgggc aggttttcgc
     9301 aacttgctgt accaggtttt tcacgatgcc ggcgttaacg ttaaacaggt cggaacgatc
     9361 catacccggt ttacgcgcta cacctgcaga gataagaacg acatctgcgc cttccagcgc
     9421 cggagtcgca tcttcaccag aaaaaccttt gattttcaca gcagtaggga tatggctcag
     9481 atcgacagcc acaccgggag tcactggagc gatatcatac agagagagtt ctgaacctga
     9541 aggcagttgg gtttttaaca gtagtgcaag cgcctggcca ataccgccag cagcgccgag
     9601 gactgcgact ttcatcctaa actccttatt atattgataa actaagatat gttgctccgc
     9661 tgccgcgacc ttaatccaca aaattgccgt ttacaatgac cacatctcaa gaatgtgtag
     9721 tcacgcaagt ttagcgttta tgcatttaat tgccgtaatc aggaacctaa cttacgtaat
     9781 taacagtcgc ttatgcatta gcgcaacatt ccaacaggtg gtgacaatat accctaccgt
     9841 tcagccaaaa caacatcaat ttgataacaa ttaatttact tttaagcaga atttgcatgc
     9901 cgtgacgcag gcatgtttct caataacgaa atttgataaa atcccgctct ttcataacat
     9961 tatttcagcc ttcttcaggg ctgactgttt gcataaaaat tcatctgtat gcacaataat
    10021 gttgtatcaa ccaccatatc gggtgactta tgcgaagctc ggctaagcaa gaagaactag
    10081 ttaaagcatt taaagcatta cttaaagaag agaaatttag ctcccaaggc gaaatcgtcg
    10141 ccgcgttgca ggagcaaggc tttgacaata ttaatcagtc taaagtctcg cggatgttga
    10201 ccaagtttgg tgctgtacgt acacgcaatg ccaaaatgga aatggtttac tgcctgccag
    10261 ctgaactggg tgtaccaacc acctccagtc cattgaaaaa tctggtgctg gatatcgact
    10321 acaacgatgc agttgtcgtg attcatacca gccctggcgc ggcgcagtta attgctcgcc
    10381 tgctggactc actgggcaaa gcagaaggta ttctgggcac catcgctggc gatgacacca
    10441 tctttaccac ccctgctaac ggtttcaccg tcaaagacct gtacgaagcg attttagagc
    10501 tgttcgacca ggagctttaa tctctgcccc gtcgcttctg gcggcgggga aaatgttgct
    10561 tatccctctc aaccccctgc tttcccctgc gattaattta acgaatagtg cgttttactg
    10621 cgacatgtca ttcacacaat gaatacatac ggtaaaaaaa gcacattatg caaaattcat
    10681 tatctaattg aaaatactag aattaacgat aaataaccgt attttcaatt cttttttgt
//
LOCUS       DAZKFU020000161        10688 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZKFU020000161 DAZKFU020000000
VERSION     DAZKFU020000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.162"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1175)
                     /locus_tag="ACHOQQ_RS16715"
                     /old_locus_tag="pgaptmp_003343"
     CDS             complement(<1..1175)
                     /locus_tag="ACHOQQ_RS16715"
                     /old_locus_tag="pgaptmp_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HGU4471555.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     "
     gene            complement(1382..1666)
                     /gene="ihfB"
                     /locus_tag="ACHOQQ_RS16720"
                     /old_locus_tag="pgaptmp_003344"
     CDS             complement(1382..1666)
                     /gene="ihfB"
                     /locus_tag="ACHOQQ_RS16720"
                     /old_locus_tag="pgaptmp_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGU4471556.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(1826..3499)
                     /gene="rpsA"
                     /locus_tag="ACHOQQ_RS16725"
                     /old_locus_tag="pgaptmp_003345"
     CDS             complement(1826..3499)
                     /gene="rpsA"
                     /locus_tag="ACHOQQ_RS16725"
                     /old_locus_tag="pgaptmp_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGU4471557.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3610..4293)
                     /gene="cmk"
                     /locus_tag="ACHOQQ_RS16730"
                     /old_locus_tag="pgaptmp_003346"
     CDS             complement(3610..4293)
                     /gene="cmk"
                     /locus_tag="ACHOQQ_RS16730"
                     /old_locus_tag="pgaptmp_003346"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGU4471558.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4466..5230)
                     /gene="ycaL"
                     /locus_tag="ACHOQQ_RS16735"
                     /old_locus_tag="pgaptmp_003347"
     CDS             complement(4466..5230)
                     /gene="ycaL"
                     /locus_tag="ACHOQQ_RS16735"
                     /old_locus_tag="pgaptmp_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGU4471559.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(5399..6682)
                     /gene="aroA"
                     /locus_tag="ACHOQQ_RS16740"
                     /old_locus_tag="pgaptmp_003348"
     CDS             complement(5399..6682)
                     /gene="aroA"
                     /locus_tag="ACHOQQ_RS16740"
                     /old_locus_tag="pgaptmp_003348"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGU4471560.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKIIYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(6753..7841)
                     /gene="serC"
                     /locus_tag="ACHOQQ_RS16745"
                     /old_locus_tag="pgaptmp_003349"
     CDS             complement(6753..7841)
                     /gene="serC"
                     /locus_tag="ACHOQQ_RS16745"
                     /old_locus_tag="pgaptmp_003349"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGU4471561.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(8040..8732)
                     /locus_tag="ACHOQQ_RS16750"
                     /old_locus_tag="pgaptmp_003350"
     CDS             complement(8040..8732)
                     /locus_tag="ACHOQQ_RS16750"
                     /old_locus_tag="pgaptmp_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGU4471562.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            8862..10622
                     /gene="ycaO"
                     /locus_tag="ACHOQQ_RS16755"
                     /old_locus_tag="pgaptmp_003351"
     CDS             8862..10622
                     /gene="ycaO"
                     /locus_tag="ACHOQQ_RS16755"
                     /old_locus_tag="pgaptmp_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGU4471563.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSNNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRDTILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         2701 a         2770 c         2540 g         2677 t
ORIGIN      
        1 agatttgcaa taaacgaggt cagactaatg ccatgaaata tgacgatttg cacgggcata
       61 agcagaagtg tgattcccag ttgcaaatga atgagggaaa caactgcacg caataccggc
      121 ggcagttgcc actcaggaca gggaaaccac tgataccaaa atatcaatgc cgcgaccgca
      181 gcggcagaga gccataaact ttgcgagaga atggcaacag gatccatcag caaaattgcc
      241 gccagacaac atatccatac atcccagcca ctccactgtc gcccacttaa cttaagcatt
      301 ccccacgtag caagcgccac aacggtacgc aatgcaggag gttgcattcc cgtcagccag
      361 gcataaaaag cagcacagca gattccgcca attaatggca tttgccagtg gatccagcgc
      421 ccaggcagaa aaatttgtcc actgcgaatg agtccggcag ccagcaacgc cgcaaaagcg
      481 atgtgcaatc ccgatatcgc cattaaatgc gccgttccag tatcacgcat gatatttttg
      541 atttctttag ggacggataa ccgttccccc atacctaaac caagaataac cgcattccac
      601 ggatagggtt gcagcgttgt ttgtagtgac gccagatact gtgcacgcag gctacaattc
      661 ggttcaataa cacttgcctg cagaaagcgg ccggtgagcg gctgatgctg ggcaatggca
      721 taacgctggc tatcaaagcc gccatcatta agttgaccat gaacggcacg aactttgagt
      781 ttcattgacc acagttgtcc ggcacaaacc gcttgcggaa gatattcgcc atacagcaca
      841 aggcctggcg cagggaatat acgtcgacct tgtagataag taatttgacc gtaatgagtg
      901 gtcatgccgt cagtagcagt gatctcaaca attgcatcct gcgtcgcgcc agttaaggtt
      961 tctcctgcca acaaaatttg ctttgctgat aatatgcccc aaacgaaaaa cagcaacgtc
     1021 agcgcgatat aacggacggt tttaacaggg ataaatgcca gtacgcaggc gaagagagtc
     1081 agaaacgcaa gggttaatgt cccaggcaat tggggcaaaa tcagcaacgg aaaaattccg
     1141 caaattatgc atacgccgac tgtcgttatt ttcatgcacc cctccctgtt tatggaaagt
     1201 gtgcaacttt gttgagcggt tgtctggtgg gaattttgcg ttccccgagg cacattcaga
     1261 agaatttaac gaaagggtga aaactgctca aaactctgcg aggcagcttc cagttcctga
     1321 ttagagaaat gaaaaaagca cccgacaggt gcttttctcg cgttcaagtt tgagtaaaaa
     1381 cttaaccgta aatattggcg cgatcgcgca gttctttacc tggtttaaag tgaggaacgt
     1441 attttccttc cagttctact ttatcgccag tcttcggatt acgtccggta cgtggtgcgc
     1501 ggtagtgcaa agagaaactg ccgaaaccgc ggatttcaat acgctcgccc tgcgcaagag
     1561 tcgaggccat atgctccagc atctctttta ctgcatcttc aaccgtcttg gcgggaatgt
     1621 gcgattgctg ggtggcaagt ctttctatca attctgactt ggtcatgatt cctccggttc
     1681 cttaaaggca aattaagcgg ctgctgttgc tgcattgatt aagggcggcc gtagccgccc
     1741 ttagtgcttg attacaggac gaaacctgca atctgtcaag taaactcaac aatcttcgga
     1801 ataaaaatcc cgaagagtca gagaattact cgcctttagc tgctttgaaa gcttcagcca
     1861 ttgcgttgtt ggagaagttt gcatcttcct gtttgttaac agttgcgatt gcatctttct
     1921 cgtcagcttc gtctttcgca cgaacagaca ggctgattgc acggttttta cgatcaacgc
     1981 cggtgaattt agcttcaact tcgtcgccaa cgctcagaac cagggtagcg tcttcaacgc
     2041 ggtcacggga tgcttcagaa gcacgcaggt aaccttcaac gccgtcagcc agttctacgg
     2101 ttgcgccttt agcgtcaact gcagttactt taccggttac gatagcgcct ttcttgttca
     2161 gagcaaccca gttgttgaac ggatcttctg cgagctgttt aacgcccagg gagatacgtt
     2221 cacgttctgc gtcaacctgc agaacaactg cagcgatttc gtcgcctttt ttgtattcac
     2281 gaactgcttc ttcgcctgca acgttccagg agatgtcaga caggtgaacc aggccgtcga
     2341 tgccgccgtc caggccgatg aagataccga agtcagtgat agacttgatt ttaccttcaa
     2401 cacggtcgcc cttattgtgg gtttccgcga actgctgcca cgggttagct ttgcactgtt
     2461 tcagacccag ggagatacga cgacgttctt cgtcgatatc cagaaccata acttccacta
     2521 catcgccaac gttaacaact ttggacgggt ggatgttttt gttggtccaa tccatttcgg
     2581 aaacgtgtac caggccttca acgccttctt cgatttcaac gaagcagccg tagtcggtca
     2641 ggttggtcac gcgaccagtc agtttggtac cttccggata acgtttagcg atagctaccc
     2701 acggatcttc gcccagctgt ttcaggccca gggatacacg ggtacgttcg cggtcgaact
     2761 tcagcacttt aacagtgatt tcgtcgccca cgttgacgat ttcgctcgga tgcttaacgc
     2821 gtttccaggc catgtcagtg atgtgcagca ggccgtcaac gccgcccaga tcaacgaatg
     2881 caccgtagtc agtgaggttc ttaacgatac ctttaacttc catgccttcc tgcaggtttt
     2941 ccagcagctg atcgcgctct gcgctgtttt cggattcgat aacggcacga cgagaaacaa
     3001 caacgttgtt gcgcttctga tccagcttga ttactttaaa ttcaagctct ttgccttcca
     3061 ggtgcagagt gtcacgcacc ggacgaacgt ctaccaggga acctggcagg aacgcacgaa
     3121 taccgttcag ctcaacagtg aagccgccct taactttgcc gttgataaca ccggtaacag
     3181 tttcagcatc ttcgtaagct ttttccagcg tgatccaggc ttcgtgacgt ttagctttct
     3241 cacgggacag cagagtttca ccgaagccgt cttctactgc gtccagagca acgtcaactt
     3301 cgtcacctac ctggatttcc agctcgccct gggcgttttt gaactgctca gccgggatgg
     3361 cggactcaga tttcagacca gcgtcaacca gtactacatc tttgtcgata gcaacaacaa
     3421 cgccacgaac gatagaaccc gggcgggttt cgatttcttt taaggactct tcaaagagtt
     3481 gagcaaaaga ttcagtcatg tttaatcttc aggtttatat ttaacgtcca cctggctcca
     3541 tgccggatgg ggttgtttaa catacccgct gtcattccat tgcaacgggg gtactgcaaa
     3601 ttcggtcgct tatgcgagag ccaatttctg gcgcgcgtat tgtagcgctt tttcaatcac
     3661 ttgctcaatg cttaaggtgg tggaatccaa cactaaagca tcggctgccg gaaccagtgg
     3721 cgctaccgct cggttacgat cgcggtcgtc gcgttctttg atctcggcca aaaggcgctc
     3781 aaagttaaca ctaaagccct tctcctgcaa ctgtagcatg cggcgatgcg cacgttcttc
     3841 cgaggaggcg tcaaggaaaa ttttcactgg cgcatcaggg aataccaccg ttcccatgtc
     3901 gcggccatcg gcaatcaggc ctggtaattc gcgaaacgcg cgttggcgac gcaataatgc
     3961 ttcacgaacg cgtgggaatg ccgcgacttg tgaagcagca ttcgccactt cctgagtacg
     4021 aatttcgccg ctgacatctt ccccttcgag gatcacttcc agattgccat tggtcgacac
     4081 aaaacgtaca tccagatggg atgccagcgg caccagcgca tcttccgaag caacatcaac
     4141 atggtgatgt aatgccgcta gtgccagtac gcgataaatt gcacccgagt ccagcagatg
     4201 ccattgcaac gcttccgcca tagccttaca caaggtgcct ttccctgcac cgcttgggcc
     4261 atcaatggta ataaccgggg caattgccgt catctttatc tccttaaaac aggcgtaccg
     4321 ttaacataac cgcgcgcatt atacgcgccg acgccacaaa tcgttaacgt tgtgtgcaaa
     4381 aaccagtttt gtctgtattc agatggggat ttattgcgca attatagcgg gctggcagaa
     4441 gaccagcccg aaagatgaca atgatttact taccagaggc gatacgatca cggatgtgtt
     4501 gcgcacgctc tgttgatggt gggtgagagt caaacatgga ctgggtgcga ccgccatcca
     4561 ggctagccag tttttcaaag ctgccaacca gcccctgggt gctgatgcca cgtttcttca
     4621 acagatcaaa ggagaaatca tctgcttcgg actctttatc acgggagtat ttcgcattga
     4681 tagcgccttc tgcgatatca cctaattgtg agcgggaaag ctgggaagcc acaccgctgg
     4741 tagctgaaat ggcatcgcgt gcggcaacga tcgcataaga agctttcatt tcagccagcg
     4801 agtgacccaa cgcgacgtgg cccagttcat ggcccagaac accttcaatt tcgttgtcat
     4861 tcatcatgtc catcaggcca ctgtagacac gaacacagcc gttcgccatc gcccatgcgt
     4921 tgacgtcgct ggtcatataa accttatagt tgaccggcgt gccattaata ttgttaccca
     4981 atgctttggc gatttttgcc agacgtttac cgtacttgct ggatttactt gcgacttgat
     5041 tgccactgtc catttcggca cagccctgat tggcaatcgc ttttgcatcg gcatcgctta
     5101 acgttgctgc tttataggca tttaaaccgg agctgatagc catattggta tcaataccgt
     5161 gggtattttg acaccctgtc agtaatgctg cagaggtcgc aatcgccagc agtaatttag
     5221 tattcttcat gtgaatcatc cgttgattaa tcatatataa atattatgta tcaacaactt
     5281 acattgacaa gcgacgctac cattaatcaa aatgactcac aaggtccgaa aaaaaacgcc
     5341 ggcaatgtgc cggcgttttg ataaagaaag atttggctat ttattggacg ttattcattc
     5401 aggctgcctg gctaatccgc gccagctgct cgaaataatc cggaaatgtt ttggccgtac
     5461 atttgggatc aagaatcgtc actggtgtat ctgacaacgc caccagcgag aaacacatcg
     5521 ccatccggtg atcattgtat gtcgcgatct cggcaaagtt cagtttttcc ggtggagtga
     5581 tacgaatgta atcgtgcccc tcttctactt ccgcaccgac tttacgcagt tctgttgcca
     5641 tcgcaaacag gcgatcggtt tctttaacac gccagttata gatattgcgc agcgtggtgg
     5701 tgccttttgc aaataacgcc gccgtggcaa tggtcatcgc cgcatcagga atatggttca
     5761 tatccatatc aatagcgttc agttcaccac gcgtgcagga aatataatca tcgccccagc
     5821 aaatggtcgc gcccattttt tccagcacat cagcaaagcg aatatcaccc tgcatactgt
     5881 tacgtccaat accggtcact tttacagtgc cgcctttgat tgctgctgct gccagaaagt
     5941 aagaagccga agatgcatcg ccttcgacca aataagtacc cggagactga taagactgcc
     6001 cgccttttac gacaaattgt tgatagtgct gattttcaat ttcaacacca aacgtcttca
     6061 tcagattgag tgtgatgtcg atataaggtt tagaaaccag atcgccttta atacgaatca
     6121 ccgtatcttc cggcgcaaga ggcgcagtca ttaacagtgc ggtgaggaat tggctggaaa
     6181 cggagccatc aacgtcaacg ttgccgccag taaagccgcc ctgtaaacgc aacggcggat
     6241 aattttcttg ttccaggtaa atgatcttcg ccccgcccag gcgcagcgca tccaccagat
     6301 gaccaattgg gcgctctttc atacgcggct caccggtcag cacaatatca ttgctaccca
     6361 gacaaagagc tgccgccagc ggacgcattg ccgttccggc gttaccgagg aacaactcca
     6421 gggcaccttc tgcgtgtaat ggaccgccgt taccgataat ttcgcaacgt gtacgatcgg
     6481 ctgaaagcgt atagcttacc cctaacgctg ttaatgcatt cagcatatgg cgcacgtcat
     6541 cgctatccag cagattggtt aatactgttt tgccgtgtgc taatgccgcc agcaataaag
     6601 cgcggttaga aacgctcttg gaaccgggca gattaatagt gccatcgaca cgagcgatgg
     6661 gttgtaacgt cagggattcc atgaactcaa ctctctacaa cagaaataaa aaccccacag
     6721 gctggctgtg gggattaagc aaaatttcgg cattagccgt gacggcgttc gaactcaacc
     6781 atgaagtctg tcagcgcttt aacgccttcc agcggcatgg cgttataaat agacgcgcgc
     6841 attccgccga ccacacggtg acctttcagt gcatgaaggc ctgcagcaaa agactcttca
     6901 aggaacaatt tgtcaagcgc actgtccgcc aactggaacg gcacgttcat ccgcgaacgg
     6961 ttagctttcg ccacgtcatt gcggtagaaa tcgctgttat caattacccc atatagcagt
     7021 tctgcttttt gctgattgat tttatccatt tcagctacac cgccgttcgc tttcagccat
     7081 ttaaagacca gaccagacag ataccaggca aatgtcggcg gcgtgttaaa catggagccg
     7141 ttatcgttga ggatggaata atccagaatc gacggacacg cgatattcgc tttgcccagc
     7201 aaatcttcac gaacgatgac gattgtcagg cctgccgggc cgatattttt ctgcgcgcca
     7261 gcgtaaatca cgccataacg gctaacgtca atcggacggg aaagaatggt tgaagagaag
     7321 tcggcggcga ccaccacatc tttgccgaag tctggcgttt cgtcgatggc gataccgtcg
     7381 atggtttcat tcgggcagta atgcatataa gcagcattat cagagagttg ccattcacgc
     7441 attggcttaa ccgcgcgcag accatcaaca gtcactttgg cgtcaaagac attaggcgtg
     7501 cagtattttt tcgcttcttt aatggcactt gccgcccagt aaccggcatc aacataatct
     7561 gcggtggttt tatcaccgag aatattcagc ggaacagcag caaactgacc gcgaccgccg
     7621 ccatggcaga acaatacctt gtagttggag gggacattaa gaagatcgcg aaaatccttc
     7681 tcggcttcct ctgcaacctg aatgaactct ttgccacggt gactcacttc catcaccgac
     7741 gtaccaagac cgttccagtc acgcagttcc tgttgagcct gtttaagcac ctctgccggt
     7801 agcattgccg gaccagaact aaaattgaag atttgagcca tttcccctca ccacgttgcg
     7861 ttgccattaa cccccagtca atcgattgcg accgcgggta atatgttatt cctgtggcta
     7921 tcggttttat cattcagtga cacgcatcgc aatgggtaaa accgttgagg gcaaaaatgc
     7981 ggcacgcgtc acacaacaga atctctcgga ttattagcaa aaaaccgata tttttgctgt
     8041 caggtcaccc gtttagcccg acttgccttc gtccattctg gatttgcaca gcgcggacat
     8101 aattgttttt cccccgcgtt catatgaatg atttcactac aacggatgca ggcatagtcc
     8161 gccgactccg gcaccacttt gtaacgctgc gtacaatcac tgggtaaaat aagtaaaccc
     8221 ggtttcacct tgtcatcttc aaagaaatag acactgattt gcccgttggt ttcgagaatc
     8281 gccagacgta cttgccccaa ctgctctaca ccgcgcaatc gtagctccat aaagaactca
     8341 aattccgtca tgttggagtt attgagtttc gaccaggcca gctcgccatc ttcaataatg
     8401 acaaccggct taccttccag aagatcttcc agtttttcac tgtgtgccat caaccacatt
     8461 accaggcggt ataacaacgc cagagtgata aagacgataa gtaccgggac catcggcaca
     8521 tcatcataaa acgccacatc tcccgccgct gaccccagcg tcagaatgat taacacttca
     8581 aacagcgaca tctgccgcac tccgcgtctt ccggtcattt tgaggaacaa aaaaaccagt
     8641 acaaaggtat acagactacg tagtgcaact tcgccaagga aatcaaaagg cactttatca
     8701 aatgccatac ggtggagatc gaacgccttc atttttatgc tcttaacgtg gtttatccgc
     8761 taaaagcata gtcaacagtt catcggcatg gcggcggtga tagcgccttt gagataaaac
     8821 ccgtattatt gcgcgctttc cgtacgacta aagtgatttt catgacgcaa acatttatcc
     8881 ccggcaaaga tgccgcactg gaagattcca tcgctcgctt ccagcaaaaa ctttcagacc
     8941 tcggctttca gattgaagag gcctcctggc tgaatcccgt gcctaacgtc tggtctgtac
     9001 atattcgcga caaagagtgc gcactgtgtt ttaccaacgg taaaggcgca accaagaaag
     9061 cggcgctggc ttctgcactc ggtgaatatt tcgagcgtct ctcaaccaac tacttttttg
     9121 ctgatttctg gctgggcgaa accatcgcca acggtccatt tgtgcattat cccaacgaaa
     9181 aatggttccc actgaccgaa aatgacgatg tgccagaagg gctgctcgat gaccgtctgc
     9241 gcgcatttta cgatccggag aatgaattga ccggcagtat gctgattgac ctacaatccg
     9301 gtaacgaaga tcgtggtatt tgcggtctgc cgtttacacg tcagtctaat aatcagaccg
     9361 tttatattcc gatgaatatc attggtaact tgtacgtttc taacggtatg tctgctggca
     9421 atacccgtaa cgaagcacgc gttcaggggt tgtccgaagt tttcgaacgc tacgtgaaaa
     9481 accgcattat tgctgaaagc atcagcttgc cggaaatccc ggcagacgtg ctggcacgtt
     9541 atccagcagt agttgaagcg atcgaaacgc tggaagcgga aggtttcccg atcttcgctt
     9601 atgacggttc gcttggcggc cagtatccgg tgatttgcgt ggtactgttt aatcctgcta
     9661 acggcacctg ctttgcctct ttcggtgcgc atcctgattt tggcgtagca ctggaacgta
     9721 ccgtgaccga gctgctgcaa ggtcgtggcc tgaaagattt ggatgtgttt actccgccaa
     9781 ccttcgatga tgaagaagtc gctgaacata ccaacctcga aacgcacttt atcgattcca
     9841 gcggtttaat ctcctgggac ctgttcaagc aggatgccga ttatccgttt gtggactgga
     9901 atttctccgg caccacggaa gaagagtttg ccacgctgat ggctatcttc aacaaagaag
     9961 ataaagaagt ttatattgcc gattacgagc atctgggcgt ttatgcttgc cgtattatcg
    10021 tgcctggcat gtccgatatt tatccggctg aagatttgtg gttagcgaat aacagtatgg
    10081 gtagtcatct gcgcgacacc atcctctcgc taccaggcag cgagtgggaa aaagaagatt
    10141 acctgaacct catcgagcaa ctggatgaag aaggttttga tgactttacc cgcgtgcgtg
    10201 agctgttggg tctggcgacc gggtcggata acggttggta caccctgcgt atcggtgaat
    10261 taaaagccat gctggcgctg gctggtggcg atctggaaca ggctctggtc tggaccgaat
    10321 ggacgatgga gtttaactca tcggtattca gcccggaacg cgccaactat tatcgctgcc
    10381 tgcaaacgtt gttattactg gcgcaggaag aagatcgcca gccgctgcaa tatctgaatg
    10441 cgtttgttcg catgtacggc gcagatgccg tggaagccgc tagtgcggca atgagcggcg
    10501 aagcggcgtt ttatggcttg caaccagtag atagcgatct gcacgcgttt gctgcacatc
    10561 agtcgctgtt gaaggcctac gaaaagctgc agcgcgccaa agcagcattc tgggcaaaat
    10621 aaaatcaaat agcctacgca atgtaggctt aatgattcgt ctgagttata ttacggggcg
    10681 ttttttta
//
LOCUS       DAZKFU020000162        10674 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZKFU020000162 DAZKFU020000000
VERSION     DAZKFU020000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.163"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(139..1731)
                     /gene="lsrK"
                     /locus_tag="ACHOQQ_RS16760"
                     /old_locus_tag="pgaptmp_003352"
     CDS             complement(139..1731)
                     /gene="lsrK"
                     /locus_tag="ACHOQQ_RS16760"
                     /old_locus_tag="pgaptmp_003352"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HGU4471564.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1810..2763)
                     /gene="lsrR"
                     /locus_tag="ACHOQQ_RS16765"
                     /old_locus_tag="pgaptmp_003353"
     CDS             complement(1810..2763)
                     /gene="lsrR"
                     /locus_tag="ACHOQQ_RS16765"
                     /old_locus_tag="pgaptmp_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGU4471565.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQILAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3012..4547
                     /gene="lsrA"
                     /locus_tag="ACHOQQ_RS16770"
                     /old_locus_tag="pgaptmp_003354"
     CDS             3012..4547
                     /gene="lsrA"
                     /locus_tag="ACHOQQ_RS16770"
                     /old_locus_tag="pgaptmp_003354"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGU4471566.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAVGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRFSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4541..5569
                     /gene="lsrC"
                     /locus_tag="ACHOQQ_RS16775"
                     /old_locus_tag="pgaptmp_003355"
     CDS             4541..5569
                     /gene="lsrC"
                     /locus_tag="ACHOQQ_RS16775"
                     /old_locus_tag="pgaptmp_003355"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGU4471567.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKTAS
                     SGKKREAA"
     gene            5569..6561
                     /gene="lsrD"
                     /locus_tag="ACHOQQ_RS16780"
                     /old_locus_tag="pgaptmp_003356"
     CDS             5569..6561
                     /gene="lsrD"
                     /locus_tag="ACHOQQ_RS16780"
                     /old_locus_tag="pgaptmp_003356"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGU4471568.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLGLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6573..7595
                     /gene="lsrB"
                     /locus_tag="ACHOQQ_RS16785"
                     /old_locus_tag="pgaptmp_003357"
     CDS             6573..7595
                     /gene="lsrB"
                     /locus_tag="ACHOQQ_RS16785"
                     /old_locus_tag="pgaptmp_003357"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGU4471569.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7622..8497
                     /gene="lsrF"
                     /locus_tag="ACHOQQ_RS16790"
                     /old_locus_tag="pgaptmp_003358"
     CDS             7622..8497
                     /gene="lsrF"
                     /locus_tag="ACHOQQ_RS16790"
                     /old_locus_tag="pgaptmp_003358"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HGU4471570.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8521..8811
                     /gene="lsrG"
                     /locus_tag="ACHOQQ_RS16795"
                     /old_locus_tag="pgaptmp_003359"
     CDS             8521..8811
                     /gene="lsrG"
                     /locus_tag="ACHOQQ_RS16795"
                     /old_locus_tag="pgaptmp_003359"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HGU4471571.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8868..9626
                     /gene="tam"
                     /locus_tag="ACHOQQ_RS16800"
                     /old_locus_tag="pgaptmp_003360"
     CDS             8868..9626
                     /gene="tam"
                     /locus_tag="ACHOQQ_RS16800"
                     /old_locus_tag="pgaptmp_003360"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HGU4471572.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9630..10544)
                     /locus_tag="ACHOQQ_RS16805"
                     /old_locus_tag="pgaptmp_003361"
     CDS             complement(9630..10544)
                     /locus_tag="ACHOQQ_RS16805"
                     /old_locus_tag="pgaptmp_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGU4471573.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         2590 a         2629 c         2793 g         2662 t
ORIGIN      
        1 ccttgaatat agtactggtg atggaacgat gaatctgcaa gctggcattt aacaagccag
       61 ctctaaaaga agggaaataa gaaaaactat actcaaaaac taacagccac ggtcatcatg
      121 atgtggctgt caatgaaact ataacccagg cgctttccat aacgatgtcg tcagtccatg
      181 atcaaccagc cccagctgat cctgataaac tgcctgccac ttatcgcgtg aatcctgata
      241 aagttcatgc ttttccgggt ctggtgtgtg cgtccgttcc cagcgaacca ggcgttctcc
      301 ggtttctgcc attgatgaaa aaattccggc accgacgcca gctgcaatgg cacatcctaa
      361 tgcggtggct tctttgacca ccggaatatt gacgggtaat cccgagacat cagcgagaat
      421 ttgactccat aatttccctt ttgaacctcc gcctgcaaag actaacgatg aaggatgaat
      481 attcgagaaa tcagcaattt gctgcaagtt acacgctgat acaatcgccg cattttcttc
      541 cagcgcacgg aacaatgtcg ctttgttaca tttatccggg tcaatggaca agttaataaa
      601 ggaaggcgca gcgtgatacc aggttttaaa gcgcattctg tcggagaaga tcggcattac
      661 gccccacgac ccaggcggca cccgactggc catctcttcc agcagcgtat aggtgtcgat
      721 gcctaaacgt tcggcaatca gtttttcttc ggcacagaaa gcatcgcgga accagcgcat
      781 ggtgagtccg gtaaaaaagc ttatagattc agcttgtacc atgccaggaa taacatgagg
      841 attaacgcgc acgttcattt ctgggtctgt caccggcgcg gctaaattta caacttgctg
      901 ccagaatgtg ccgccaagaa ccgcggtttg tgccggacgc acaacgccta accccaggca
      961 accaagctgc acgtcgcctc ctccaacgac caccggagtg cccaccttca gaccgcagag
     1021 ttccgccgct tgtgaactta ccacgcccag caatgtgccg gtttctttga caggagaaag
     1081 aatatcggca cgcaggccag ccatatccag caatgcaggt ttccagtcac ggatggttag
     1141 atcaagaagt cccgtggtgc cagcgttaga gggatccacc gccagttcgc cgctgagcat
     1201 ataggccagc cagtcgctga tcatggtgat ggttgatgcc tgacggtaaa tatcggaacg
     1261 atggtgcgcc agccaaagta atctggggat ggcacttaaa gccagtgttt gtccggtcgc
     1321 gcgataaact tcgttttcaa aggtattgtt gtgcagttct ttaagttcgc taacttcgcg
     1381 cgctgctctg gcatccacat tggcgcaggc ccagatcggt gttccttcat tgttatataa
     1441 aacaatgcct tcacgcatag aacatgccga aacggcagcg atatactccg ggtctatgcc
     1501 ggcgttgtgc agcgcctggc gcatacactc acacgccagt tgccagtttt tgttgagatc
     1561 aaattccata gaaccaggaa cgtccggtac tgccagatgc cgccactccg cctgtcccac
     1621 tgctatttga ttgccttcca ggtcgaatat cacagcccga atacttccgg tgcctgcatc
     1681 cagcgccatc aggtagtact ttgattctga aggggtaaag agtcgagcca ttagattatc
     1741 ctcggcttat gcgttctgtc tgttcctcta tacgttctcc atcattcccg gtaataaggt
     1801 catgcaaatt taactacgta aaatcgccgc tgctgtgtcc tgatcggtaa ccagtgcgtt
     1861 gatataaccg cctttcattg cagcggcaat tgcttcggct ttattttctc cccctgccac
     1921 gccaacccgg acgggtatgg tcttcagcgc gcttaaaggt aagccaatca gttcgttatg
     1981 tattttgata tccgtgacaa cgtcaccttt tgcatcaaaa aagtagccta aaatgtcgcc
     2041 aaccgcccct tttcggccaa tcattaactg ttcgccctgg ctgatataac cggagcgaat
     2101 gatcgtcgcg tcgtcttgtt gactcacggc cccaatgccg acaatcgcca catccgctgc
     2161 ttgcgcggct aacagaacat ctttgacgca attttcattt tttagcgtac gggcaatgtc
     2221 agcggaggat gcccgcaacg gagccggaat aatattcaca ctgcacgccg cgttaagctg
     2281 cccgataccc gtcatatagg aaccgacgcc accggagagc gtgaccaggc gaatttgctg
     2341 tgacgaaata aaaccactta agcgttgcag cgtattcatg gttgcctcgc caaaaccaat
     2401 cgccaggatc tgttgtggtt gaagtaaact catcaacata tgcgccgcgc ctatccccag
     2461 tcgcccgccg acatcagcat ccgcaagccc agggatcacc cggacatgtt gcagcgaaaa
     2521 ctgacgacgt aattgtgttt catattccag acagccttca aagcgagaat taatctgcac
     2581 gcgaatgatg ccggactgat gccctttctc cagtaatcgc gacactttca aacgggtcag
     2641 gccgagacga tcgctgatct cactctgggt cagcccgtcg tggtagtaaa accacgcgat
     2701 ccgcgcgacc tgttcttctt cacacattcc ctgttctgaa attgccgaat cgttgattgt
     2761 cataatttat tcttcacttt gaacatattt aaatctttaa tgcaattgtt cagttcttgc
     2821 gcatttatat ctgtgatggc aaccacagtt tgactctacg agcatgaaca aacgcaaccg
     2881 tgaaaatcaa aatagcataa attgtgatct attcgtcgga aatatgtgca atgtccacct
     2941 aaggttatga acaaattaaa agcagaaata catttgttca aaactcacct gcaaaactga
     3001 acgggggaaa tatgcaaacg agtgataccc gcgcgttacc gctactttgc gcccgctcgg
     3061 tttataaaca gtattcaggg gttaatgtcc tgaaaggcat cgattttacg ttgcaccagg
     3121 gggaggtcca cgccctgctc ggcggcaatg gtgccggtaa atcgacgtta atgaagatta
     3181 ttgccggtat tacccctgct gatagcggta cgctggagat tgggggcaac aactacgtca
     3241 gattaacgcc agttcatgct catcagctgg gtatttatct cgttccccag gaaccgctgc
     3301 ttttcccaag cctgtcgata aaagaaaaca tcctgtttgg gctggcaaaa aaacagctct
     3361 ccatgcagaa aatgaagaac ttgctggcgg cgctgggctg ccagtttgat ctgcatagtc
     3421 tggcaggatc gctggatgtc gccgatcgcc aaatggtgga aatcctccgc gggctgatgc
     3481 gcgactcgcg gattctgatc ctcgatgaac ctaccgcctc gcttacccct gcggaaaccg
     3541 aacgcttgtt tagtcgcttg caagagctgc ttgctactgg cgtgggtatt gtttttatct
     3601 cgcataagct gccggaaatt cgccagattg ccgatcgaat tagcgtgatg cgcgacggaa
     3661 ccatcgcctt aagcggcaaa accagcgaac tgtctaccga cgacattatt caggccatca
     3721 cgccagtggt acgggaaaaa tcgctctctg ccagccaaaa attatggctg gagttacctg
     3781 gtaaccgccc acaacatgcc gtaggaacgt cggtgctgac actggaaaat ctgactggcg
     3841 aaggttttag gaatgtcagc ctgacgctaa atgctggaga aattctgggt ctggctgggc
     3901 tggtgggtgc cggacgcaca gaactggccg agacgctcta tggtctacgt actttgcgtg
     3961 gcggacgcat tatgctgaat gataaagaga tcaatagatt ctccactgga gaacgtttac
     4021 tgcgcggtct cgtttatctg ccggaagatc gccagtcctc cggactgaat ctcgatgctt
     4081 cgctggcctg gaacgtctgc gcccttactc ataaccttcg tggattctgg gcgaaaaccg
     4141 cgaaagataa tgccaccctg gaacgatatc gtcgggcgct gaatattaaa tttaaccaac
     4201 cggaacaagc tgcacggaca ttatccggtg gcaaccagca aaaaatcctg attgccaaat
     4261 gcctggaagc ctcgccgcaa gtattgatag tcgatgagcc gacgcgcggc gtggatgtct
     4321 cggcccgtaa tgatatctac cagctgttac gcagcatcgc cgcacaaaat gtggctgtgc
     4381 tgcttatctc ctccgacctg gaagagatcg aactgatggc agatcgtgtg tatgtgatgc
     4441 atcagggcga aattacccac tctgcactga ccgggcgcga tattaatgtc gagactatta
     4501 tgcgcgttgc cttcggcgat agtcagcgtc aggaggcgtc atgctgaagt ttattcagaa
     4561 caaccgtgaa atcacggcac tgctggcggt ggtgctgctg tttgtattac ccggttttct
     4621 cgaccgccag tatttaagtg tgcaaacgct gaccatggtt tatagcagcg cgcaaatcct
     4681 gatcctgctg gcaatgggcg cgacgctggt aatgcttacg cgcaatattg atgtttcagt
     4741 gggttcgatt accggaatgt gcgcggtgct gttggggatg ttactgaacg cggggtattc
     4801 actacctgtt gcttgtgtcg cgactttact gcttggtttg ctcgcaggat ttttcaacgg
     4861 tgtcctggtc gcgtggctaa aaatccctgc cattgttgcc acccttggca cgttagggtt
     4921 gtacagaggc atcatgttgc tgtggactgg cggtaaatgg attgaggggt tacccgccga
     4981 actgaaacag ctctccgccc cgctgctgtt tggtgtttcc gcgattggtt ggttgacgat
     5041 aattctggtg gcatttatgg cctggctgtt ggcaaagacg gcgtttggac gcagttttta
     5101 tgccacgggc gataatttac agggcgctcg tcaactgggc gttcgtacgg aagccattcg
     5161 cattgtggca ttttcgttga acggctgcat ggcggcactg gcgggaattg tgtttgcttc
     5221 gcagattggt tttatcccca accagaccgg taccgggctg gaaatgaaag caattgcagc
     5281 ctgcgtgctg ggcggtatta gtttgctcgg cggttccggt gcgatcatcg gtgcggtact
     5341 cggcgcatgg ttcctgacgc agatcgatag cgtactggtg ctgttgcgca ttccggcatg
     5401 gtggaatgat tttatcgcgg gtctggttct gctggcggtg ctggtgtttg atggacgcct
     5461 gcgttgtgcg ctggaacgta atctgcggcg gcaaaaatat gcccgcttta tgacgccacc
     5521 gccatccgtt aaaactgctt cgtcaggtaa aaaacgggag gctgcataat gcgtattcgc
     5581 tacggttggg aactggctct tgccgcactg ctcgttattg agattgtctc atttggtgca
     5641 attaacccgc ggatgttaga tctcaatatg ttgctgttca gcaccagtga ctttatctgc
     5701 attggcattg tcgccctacc gctgacgatg gtgattgtca gtggcgggat cgatatttcg
     5761 tttggttcga ccatcggcct ctgcgccatt gcattgggcg tactgtttca aagtggtgtg
     5821 ccgatgccgc tggcgattct cctgggctta ctgctcggcg cattgtgcgg gctgatcaac
     5881 gccggattaa ttatctatac caaagttaac ccgctggtga ttacgcttgg cacgctgtat
     5941 ctgtttgccg gaagtgccct gctgctttcc gggatggccg gagcgacggg gtacgaaggt
     6001 attggtggat tcccgatggc gtttacagat ttcgctaacc tggatgtgct tggactcccc
     6061 gttccgctga ttatctttct gatatgtctc ctcgttttct ggctctggct gcataaaacc
     6121 catgccggac gtaacgtgtt tttgattggg caaagcccgc gcgtggcgct ttatagcgcg
     6181 attccagtta accgcacctt atgtgcgctc tatgccatga cggggctggc gtctgcggtc
     6241 gccgctgtgc tgctggtgtc gtattttggt tcagcacgtt ccgatctcgg tgcgtcgttt
     6301 ctgatgcccg ccatcaccgc cgtggtgctt ggcggggcca atatttatgg tggttccggt
     6361 tccattatcg gcaccgccat tgcggtttta ttagtgggat atttgcaaca aggtttgcaa
     6421 atggcaggag tgccaaatca ggtgtccagc gccctttccg gtgcgctact tatcgtcgtt
     6481 gtcgtaggtc gttccgttag cctgcatcgc cagcaaatta aagagtggct ggcgcgtcgg
     6541 gccaataacc cattgccata aaggatatct tcatgacact tcatcgcttt aagaaaatcg
     6601 ccttacttag cgctcttggc attgccgcaa tctctatgaa tgtgcaggcc gcagagcgta
     6661 ttgcatttat tcccaaactg gttggcgtgg gattttttac cagcggtggc aacggcgcac
     6721 aacaagcggg taaagagctg ggcgttgatg tgacctacga cgggccgaca gaacccagtg
     6781 tttctggtca ggtacagttg attaataact tcgtcaatca gggttataac gccattatcg
     6841 tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa acgcgccatg caacgtggtg
     6901 tgagagtgct gacctgggac tctgatacta aaccggagtg ccgctcttac tacattaatc
     6961 agggaacgcc cgcccagttg ggaggtatgt tggtggatat ggcggcgcgt caggtgaata
     7021 aagacaaagc caaagtcgcg tttttctact caagccccac cgttacggac caaaaccagt
     7081 gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc tggctgggaa attgtcacta
     7141 cgcagtttgg ctataacgat gccactaaat cattacaaac cgcagaagga atattaaaag
     7201 cgtatagcga tctcgacgcc attatcgccc ccgatgccaa cgccctgccc gctgccgcac
     7261 aagccgcaga aaacctgaaa aatgacaaag ttgcgattgt cggattcagt acgccaaacg
     7321 tgatgcgtcc atatgtggaa cgcggcacgg tgaaagaatt tggcctgtgg gatgtggttc
     7381 agcaaggcaa aatttcagtt tatgtcgcgg atgcattatt gaaaaaagga tcaatgaaaa
     7441 cgggcgacaa gctggatatt cagggcgtag gtcaggtgga agtctcgcca aacagcgtcc
     7501 agggctatga ctacgaagcg gatggtaatg gcatcgtact gttaccggag cgcgtgatat
     7561 tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc attacttaac cggagtaagt
     7621 tatggcagat ttagacgata ttaaagatgg taaagatttt cgtaccgatc aaccgcaaca
     7681 aaatatccct tttaccctga aaggttgcgg tgcgctggat tggggaatgc agtcacgctt
     7741 atcgcggata tttaatccga aaacgggtaa aaccgtgatg ctggcttttg accatggtta
     7801 ttttcaggga ccgactaccg gacttgaacg cattgatata aatatcgccc cgctgtttga
     7861 acatgccgat gtattaatgt gtacgcgcgg tattttgcgc agcgtagttc ccccggcgac
     7921 caataagccg gtggtactgc gggcgtcagg tgcgaactct atcctggcgg aattaagcaa
     7981 tgaagccgtg gcgttatcga tggatgacgc cgtgcgcctg aacagttgcg cggtggcggc
     8041 gcaggtttat atcggcagcg aatatgaaca tcagtcgatc aaaaatatta ttcagctggt
     8101 tgatgccgga atgaaagtgg gaatgccgac catggccgtg actggcgtgg gcaaagatat
     8161 ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc gccgctgaaa tgggggcgca
     8221 aattatcaaa acctattatg tcgaaaaagg ttttgaacgg attgttgccg gatgtccggt
     8281 acccattgtt attgctggcg gtaaaaaatt accggagcgc gaggcgctgg aaatgtgctg
     8341 gcaggctatc gatcagggcg cttctggtgt ggatatgggg cgtaatattt tccagtctga
     8401 ccatccggtg gcgatgatga aagccgtaca ggcggtggtt caccataacg aaacggctga
     8461 tcgggcatat gaactctatc tgagtgaaaa acagtaactg cggatttaag gagaagaatt
     8521 atgcacgtca cactggttga aattaacgtt catgaagaca aggttgacga gtttatcgaa
     8581 gtttttcgcc agaaccacct gggctctgta caggaagaag gcaatttgcg cttcgatgtc
     8641 ttacaggacc cggaagtgaa ttcgcgcttt tatatctacg aagcctataa agatgaagac
     8701 gcagtggcgt tccataaaac cacgccccac tacaaaacct gtgtcgcgaa actggaatct
     8761 ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt tgatgccgtg aggcgaattt
     8821 atcagtttta tctacaattg gggtaacgcg ctgacgggag taaataaatg tctgactgga
     8881 acccctcttt atatctgcac tttgccgctg aacgatcgcg tccggcggtg gagctgcttg
     8941 ccagagtgcc gctggagaat atcgaatatg ttgccgatct tggctgtggc ccaggtaaca
     9001 gcaccgccct gctgcatcaa cgttggcctg ctgccaggat aacgggcatc gactcgtctc
     9061 cggcgatgat tgctgaagcg cgcagtgctt tgccagactg ccagttcgtg gaagcagaca
     9121 tccgcaactg gcaaccggaa caggcactcg atctgatttt tgctaatgcc tcactgcaat
     9181 ggctgcccga ccactacgaa ttgtttcctc acttggtttc tttacttaat ccgcagggcg
     9241 tgctggcagt acagatgcca gataactggc tggagccgac ccatgtgctc atgcgcgaag
     9301 ttgcctggga acaaaactac ccggatcgcg ggcgtgagcc gttggctggc gttcatgctt
     9361 actacgatat tttgagcgaa gccggatgtg aggtcgatat ctggcgaacc acttactatc
     9421 accagatgcc gtcgcaccag gcgattatcg attgggtaac agccactgga ttacgtccgt
     9481 ggttacagga tctgaccgag agcgaacagc agctttttct tacgcgctac catcagatgt
     9541 tggaagagca gtatccactg caagagaacg gacagatact gctggcattt ccgcgtctgt
     9601 ttattgttgc ccgccgtacg gagtaaatat cacgtcagtt ggtaatggcg atcgggaaga
     9661 atttttgctg gaatttcggc ttcatcgttc atctgtaaga ggtcaatttc aatagcgttg
     9721 cagatggcat ccagcggtaa atcattgttt tcagtaccga acggatcttc cagttcttcc
     9781 gccagacaat ccagcgaaat aaaagtatag gaaatcagca cagagataaa aggcgtcatg
     9841 taatgcaggt ccacgaccag cgcgaacggc agcatgatac aaaacagata aacggtacga
     9901 tgcaaaatca gggtgtaggc aaagggaatt ggcgtgtagg caatgcgctc gcagcctgcc
     9961 agaaccgctg aaatatcgtt aagccgatcg ttgaggctaa taaacaggat atctgaaagt
    10021 tgtccattgc gtcgctgaac cgccaaccac tcccccatta ttaacaagat gcggttagct
    10081 ggagagtttg aagccagtac acgctgaaga tcttcagtct tgagataatg agccagcact
    10141 tccgcctgtg gctgtttgcg taatgtcatg cgtaaacagt gggcgaaggc gatttgcagc
    10201 cgggcaaact cccttacact tgccgaatct ggcaatgtcg tttttacctc gcgcagtaac
    10261 gaccgtgagg caatcatcaa ctgcccccaa agttttcgcg cttcaacgta acgggcgtaa
    10321 ccggcattat tacgaaaacc aagaaaaatg gcgatggcga caccgagaat gctgaacggt
    10381 gcgagggtga atttgatgcc cagatgcgtg taccagggca gcatgaaaat aacagcgata
    10441 gaaaagagaa aattgagtag taagcgcgag gatatcttgg ataatactga gccatgccag
    10501 acaaaaatac ggcgcagcca gtgttgttgt ggacgaacaa tcatggttat cttcaggcgt
    10561 ggaaaagtcg ccctattaaa cgtgattacg atcacattct caagacacga cttacaaatt
    10621 acctacaagc ttacaacatt aacaggcgct gcatgtggca cctgttaatg ataa
//
LOCUS       DAZKFU020000163        10662 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZKFU020000163 DAZKFU020000000
VERSION     DAZKFU020000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.164"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..752
                     /gene="yghS"
                     /locus_tag="ACHOQQ_RS16810"
                     /old_locus_tag="pgaptmp_003362"
     CDS             39..752
                     /gene="yghS"
                     /locus_tag="ACHOQQ_RS16810"
                     /old_locus_tag="pgaptmp_003362"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4471574.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPIIGASFGRYLRGKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            784..1542
                     /locus_tag="ACHOQQ_RS16815"
                     /old_locus_tag="pgaptmp_003363"
     CDS             784..1542
                     /locus_tag="ACHOQQ_RS16815"
                     /old_locus_tag="pgaptmp_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471575.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1588..2937
                     /locus_tag="ACHOQQ_RS16820"
                     /old_locus_tag="pgaptmp_003364"
     CDS             1588..2937
                     /locus_tag="ACHOQQ_RS16820"
                     /old_locus_tag="pgaptmp_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGU4471576.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAAHELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            2937..3761
                     /locus_tag="ACHOQQ_RS16825"
                     /old_locus_tag="pgaptmp_003365"
     CDS             2937..3761
                     /locus_tag="ACHOQQ_RS16825"
                     /old_locus_tag="pgaptmp_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471577.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQARFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            3773..4333
                     /locus_tag="ACHOQQ_RS16830"
                     /old_locus_tag="pgaptmp_003366"
     CDS             3773..4333
                     /locus_tag="ACHOQQ_RS16830"
                     /old_locus_tag="pgaptmp_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4471578.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLDKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4364..5434
                     /gene="lptF"
                     /locus_tag="ACHOQQ_RS16835"
                     /old_locus_tag="pgaptmp_003367"
     CDS             4364..5434
                     /gene="lptF"
                     /locus_tag="ACHOQQ_RS16835"
                     /old_locus_tag="pgaptmp_003367"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4471579.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTDNRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWIVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5431..6510
                     /gene="lptG"
                     /locus_tag="ACHOQQ_RS16840"
                     /old_locus_tag="pgaptmp_003368"
     CDS             5431..6510
                     /gene="lptG"
                     /locus_tag="ACHOQQ_RS16840"
                     /old_locus_tag="pgaptmp_003368"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4471580.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSLGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQAMQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKMWILHGVNHKKWLNGKETL
                     DTSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQY"
     gene            complement(6545..7717)
                     /locus_tag="ACHOQQ_RS16845"
                     /old_locus_tag="pgaptmp_003369"
     CDS             complement(6545..7717)
                     /locus_tag="ACHOQQ_RS16845"
                     /old_locus_tag="pgaptmp_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4471581.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     DGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7717..7965)
                     /locus_tag="ACHOQQ_RS16850"
                     /old_locus_tag="pgaptmp_003370"
     CDS             complement(7717..7965)
                     /locus_tag="ACHOQQ_RS16850"
                     /old_locus_tag="pgaptmp_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4471582.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(7997..8911)
                     /locus_tag="ACHOQQ_RS16855"
                     /old_locus_tag="pgaptmp_003371"
     CDS             complement(7997..8911)
                     /locus_tag="ACHOQQ_RS16855"
                     /old_locus_tag="pgaptmp_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4471583.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGIAGGYDW
                     QRVQQLAAEARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8908..10596)
                     /locus_tag="ACHOQQ_RS16860"
                     /old_locus_tag="pgaptmp_003372"
     CDS             complement(8908..10596)
                     /locus_tag="ACHOQQ_RS16860"
                     /old_locus_tag="pgaptmp_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HGU4471584.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHTWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
BASE COUNT         2558 a         2668 c         2800 g         2636 t
ORIGIN      
        1 acgtaatatt caatacatta tttgctgccg ggtttttcgt gaatcaacgt aatatgtcaa
       61 taattaattc cacgccagtg cgtgttattg ccattgtagg atgtgatggt tcaggtaaat
      121 cgaccctcac ggcaagcctg gtaaatgaac tggcagcaag aatgccaaca gaacacattt
      181 atctcgggca atcgtccggg cgaattggcg aatggatttc acagctgccc attattggcg
      241 catcttttgg gcgttatctg cggggtaagg cagctcatgt gcatgaaaaa ccctcaacac
      301 cgcctggcaa tattactgct ctggttatct atctgctttc ctgctggcgg gcgtacaagt
      361 ttcgcaaaat gttgtgtaaa agccagcaag gctttctgct catcaccgac cgctacccgc
      421 aggttgaagt gccgggtttt cgctttgatg gcccgcaatt ggcaaaaacc acgggcggta
      481 acggttggat aaaaatgtta aggcagcgcg agctgaagct gtaccaatgg atggcgtctt
      541 atttgcccgt attgttgatt cgtcttggca ttgatgaaca aaccgcgttt gcgcgtaaac
      601 ctgaccacca actggcagcg ttacaggaaa aaatcgccgt tacgccgcaa ctgacattca
      661 atggcgcaaa gatccttgaa ctggatggtc ggcaaccagc cgatgaaatt ctgcaagcgt
      721 cactacgcgc aattcacgcc gcgctttcct gatcaccaaa atggataggg gatgatttaa
      781 cgaatggacg cactacaaac acaaactgtt aatagtacaa ccgcgccgca gcccaactac
      841 attccggggc tgattgcggt ggtcgggtgt gatggcaccg gtaaatccac actgaccacc
      901 gacctggtga aatcgctgca acaacactgg caaaccgagc ggcgctatct ggggctgctc
      961 tccggcgaag acggcgacaa aatcaaacga ttgccgttgg ttggcgtctg gctggaacgg
     1021 cgactggcgg ccaaatcctc gaaaacccaa agcatgaaaa ccaaatctcc ggcgctatgg
     1081 gcggcggtga ttatgtactg cttctcgctg cgaagaatgg cgaatctacg caaggttcag
     1141 cgactggcgc aaagtggcgt tctggtggtc agcgatcgct tcccgcaggc tgaaatttcg
     1201 ggcttttatt atgacggacc ggggattggc gtcgaacgtg cgaccgggaa aatcagcatg
     1261 tttctggcgc agcgcgaacg gcgtttatac caacaaatgg cgcaatatcg cccggaatta
     1321 attattcgcc tgggcattga tattgagact gccatctccc gcaagcctga ccatgactat
     1381 gccgagctgc aggacaaaat cggcgtcatg tcgacgattg gctataacgg cacaaaaatt
     1441 cttgagattg attccagagc gccctacagc gaagtgctgg aacaggcaca gaaggcggtt
     1501 tctctggttg ccatcgtttc tgaccgccga agtttaactt aaggctggcg ggatactttt
     1561 tactatcaac gataaggtca ggctgaattg gcgggtttta acatcaaaca ttggtttgca
     1621 gatggcgcgt ttcgcaccat tattcgcaat agcgcctggt taggctccag taatgtcgtg
     1681 agcgccttgc tgggtctgtt ggcgctctcg tgtgccggta aagggatgac gcccgccatg
     1741 tttggcgtac tggtgattgt acaatcgtac gccaagtcga tcagcgattt tattaagttt
     1801 cagacatggc agctggtggt tcagtacgga acaccagcat taaccaacaa taatccgcag
     1861 caatttcgca atgtcgtctc attttccttc tcgctggata tcgtcagcgg cgcggtggcg
     1921 attgtcggcg gcattgcctt actgccattc ctttcccatt cattaggtct ggatgaccaa
     1981 agcttttggc tggcggcgct gtattgcacg ctcattcctt caatggcttc ctccacgccg
     2041 accggcattc tgcgtgcggt agatcgcttt gatttgattg ctgtacagca ggcgacgaaa
     2101 ccttttctgc gcgcagcggg gagcgtcgca gcctggtatt ttgactttgg ttttgcgggt
     2161 tttgttattg cctggtacgt gtcgaatctg gttggcggca ccatgtactg gtggtttgcc
     2221 gcgcacgaat tacgccgcag aaatatccat aacgccttca aattgaatct gtttgagtct
     2281 gcccgacaca ttaaaggcgc gtggagtttt gtctggtcaa ccaacattgc ccactccatc
     2341 tggtcggcgc gtaactcgtg cagcacagtg ttggtgggga tcgtgttagg acccgctgcc
     2401 gccgggttat ttaaaatcgc catgacattc ttcgacgccg ccggaacgcc agcgggttta
     2461 ctgggtaaaa gtttttaccc agaggtgatg cgtttagatc cgcgcaccac cagaccgtgg
     2521 ttgttaggcg tgaagtccgg tttactggcg ggcggaatcg gtattctggt ggcgctcgcg
     2581 gtgttgatcg tcggcaagcc gctaatttcg ctggtgtttg gcgttaagta tctcgaagcg
     2641 tatgacctga ttcaggtgat gctgggcgca attgtgatct cgatgctggg cttccctcag
     2701 gagtcattgc tattgatggc gggaaaacag cgcgcttttc tcgtggcgca aaccatcgcc
     2761 tcaatcggct acatcgtatt gctgttcatg ttctgtcatc tgtttggcgt gctgggagca
     2821 gcgtttgctt atttcggcgg tcaatgtctg gatgtggtgc tttcgctaat tccgacctta
     2881 aaggcatttt ttcagcgcca ttccttgctt tataacgcag ccggagagaa atcctgatga
     2941 tgagaaaata tttcccgctt gaggcgagcg agcgtctatt tgttgcgatt gaagaagatg
     3001 atgtcgttga tgcacaggtc agtttgccgc cgactatcgc actaagttgt accaccgaaa
     3061 tcatccatga taattacgcg ttatgcctgc aattctggct caatggcgtt gatcgtcagg
     3121 aattacttcg tttaattcgc aaacaagcca aaggcgatga gctgacgaca gacgagcgta
     3181 aacagtttaa atacatgcga gcgcgctaca agcatctacg ttttgcacaa cggctgtatt
     3241 taaaaaagca tcaggccaga tttttattcg gcaaaacgac cgtttttctg ggacgttttc
     3301 aggacggatt tcgtaacggt aagaaaaata ttgtgtcgta ttacggcaac ctgttacgaa
     3361 tttatttaag ctctccggtc tggtcgttgg tgaactattc attacgccat agccagttag
     3421 aaagcgtaag cggttttatt gcctatcggc aaaagcaaat gcataccctg aaagagatta
     3481 ttgccaaacc gcgactaacc ggcagggaat ttcatgatgt gcgaaaaatc atcagccagc
     3541 aggtttctta ttacgacacc ttaagatcgc ttgatcctga aaataaagag gcgttacaga
     3601 tttcccgttt cctcgccgcc attaatggcc tgatggggga taaacacgac gacatggtgg
     3661 cggatgatat ggaaaaccgc cagtcgtatg atgcaccggt ggcgctggat agcgatattc
     3721 gccagcgctt agagctgctg atttcgcgtt ttccgctata accggagcaa tgatgtcatc
     3781 tcgtttgatt attgcgctga ttatcatgct cttagccccg ggtgtgcagg cacacaattt
     3841 cgtcactggc aaaacggtta cccctgttta tattcaggaa ggcggcgagc tgcttctgaa
     3901 tagcgacgac gaaattcact accaaaagtg gaacagcaca caattggcgg gaaaagtccg
     3961 cattatccag tatatcgccg ggcgtaagtc agcgaagaag aagaactcac tgttaattaa
     4021 agcggttgaa gcggcaaact ttccacagga tcgttttcag cccaccacca ttgtgaacac
     4081 tgatgatgcc atctttggta ctggctattt tgttgttggc aaaatcgaaa aaaataaacg
     4141 ccgctatccg tgggcgcaat tcgttattga cggcaatgga cagggacgcg ttgcctggcg
     4201 cttgccggag cagagttcaa ccattctggt gctggataaa gccgggcaaa ttcagtgggc
     4261 aaaggacggc agcctgacgc cggaagaggt agatcatgtc attgcgctgg cgcagaaact
     4321 gatcaatgaa taaaaaattc aaaggattga gggcgttagt gagatgaagc tggttgaaca
     4381 ctacatcatg cgtggtacgc gccgtctggt gctgattatc gtcggttttc tgatctttat
     4441 tttcgccagc tactccgcac agcgttatct gaccgaagcg gcaaatggga cattagcact
     4501 tgatgttgtg ctggatatcg ttttctacaa agtgctgatt gcactggaga tgttgttacc
     4561 tgttggactg tatgtgtcag ttggcgtgac gctagggcag atgtataccg attcggaaat
     4621 taccgctatc tctgcggcgg ggggcagtcc gggacgcttg tacaaagccg ttctttatct
     4681 ggcgataccg ctaagtattt ttgtcaccct tctgtcgatg tatggtcgtc cgtgggctta
     4741 tgcgcagatt tatcaactgg agcaacagtc gcagtcggag ttggatgttc gccagttgcg
     4801 ggcaaagaaa tttaacacta acgataacgg acgaatgatc ctttcgcaga cggttgatca
     4861 ggataataat cgcctgactg acgcgctgat ttatacttct actgacaatc gaacccgcat
     4921 tttccgcgcc cgttcggttg atgtggttga cccatctccc gagaaaccga ccgttatgtt
     4981 gcacaacggc actgcctatc ttctcgatca tcaggggcgt gacgacaacg aacagatcta
     5041 ccgtaatctg caattacatc tgaatccgct ggatcaaagc cctaacgtca aacgcaaagc
     5101 aaaatcggtc acggagctgg cgcgctccgc ctttcctgcc gatcatgccg aactgcaatg
     5161 gcgacaaagc cgtggcctga ccgcattgtt gatggcgctg ctggccattt cattaagtcg
     5221 ggtaaaaccg cggcaagggc gattttcaac gttattgcca ctgacgttgc tgtttgttgc
     5281 cattttttat ggcggcgacg tctgccgtac gctggtggct aacggtgcga ttcccctcat
     5341 tcctggtttg tggatagtac ccggactcat gctaatgggc ctgctgatgc tggtcgcacg
     5401 cgacttctct ttgctgcaga aattttcccg atgaatgttt tcagtcgcta tttaatccgt
     5461 catctctttc tcggttttgc cgccgccgca gggttattgc tgccgctttt tactaccttc
     5521 aacctgatta acgaactgga tgatgtcagc ctgggcggtt atcgctggac tcaggcggtg
     5581 ctggtggtgc tgatgacctt accgcgcaca ctggtggagc tttcgccgtt tatcgcctta
     5641 ttgggaggga ttgtcggcct ggggcagcta tcgaaaaaca gtgagcttac cgccattcgc
     5701 agcacggggt tttctatctt ccgtattgca ctggtggcgc tggttgccgg gatattgtgg
     5761 actgtttcgt taggcgcgat agatgagtgg gtagcgtcgc cattgcagca acaggcgatg
     5821 caaatcaaat cgaccgccac tgcgttgggg gaggacgatg atattaccgg caatatgctg
     5881 tgggccaggc gcggtaatga atttgtgacg gtgaaatcgc tgaacgagca gggccagccg
     5941 gtgggcgtgg agatatttca ttatcgcgac gatctctcgc tcgaatccta catttatgca
     6001 cgcagtgcca ccattaaaga cgacaaaatg tggatcctgc atggtgtgaa tcataaaaaa
     6061 tggcttaacg gtaaagaaac gctggacaca tcagataatc ttgcctggca atcggccttc
     6121 accagtatgg atcttgaaga gctatcgatg ccggggaata ctttttctgt ccgtcagctt
     6181 aatcattaca tccattattt gcaggaaacc gggcaaccca gcagcgaata ccgccttgca
     6241 ctgtgggaaa aactggggca acccatcctg accctggcga tgattttgct ggctgtgccg
     6301 tttaccttta gcgccccgcg ctcgccaggg atgggtagcc gtctcgctgt aggcgtcatc
     6361 gttggcttac tcacctggat cagctatcaa atcatggtca atctgggatt gctatttgcg
     6421 ttaagcgcac ctgttaccgc gctcggttta ccgatagcgt ttgtgctggt ggcgttgagc
     6481 ctggtgtatt ggtatgacag acaatattaa acccgccgtg gcgcaacacc acggcgggct
     6541 cacattactg cttcagagta gcaaatgcct gaatgatggc atcgatttgc tcacgactgt
     6601 gggcagcgtt tacgctacag cgcagcagcg tgatgcctgc aggcgcggca ggtggcagga
     6661 taagattcac ataaacgccc agcgaaataa gctcgcgcca gatgcgtaaa ccgtcctctt
     6721 tcgaaccgat tattaccggc actaccgggc taatatgtga acttaactca taacccagtt
     6781 tttgcagacc gtcatacaga tggttggcat tatccatcag cttttgccgc agctctggtt
     6841 gtgaggcaat ggttcttaac gacgagcgca cggtcgcaat gcaggacgga gaaggagagg
     6901 cggtaaaaat atacgggcgg ctgccgtagc gcagcacttc catcgcttcc gagcctacag
     6961 caaaaccgcc aatcgaggcc agacttttac tgaaggtgcc aacgataata tccacatcat
     7021 cttcaacgcc gacagcttca gccagtccgc gccccgttgc gcctaacacg ccaaacgaat
     7081 gcgcttcatc gacaatcaaa taaccaccga gacgacgttt gatatcaaca atttccgcca
     7141 gcggcgcaac gtcaccgagc atactgtaga tgccctcaac gatgataatc gcctctttcg
     7201 cccgttcacc gaggcgaacc atgcgccgtt ccagatcttt agcatcgtta tggcggaaac
     7261 gaataatttc cgcgccaccg agagaacagg cgtcataaat actggcgtgg ctgtcggcat
     7321 ccagtaatac cacggcgtta tggtcggcaa gtgcgctgat gacgcctaaa ttagcggtat
     7381 aaccggttga aaaaacgata gcggtagggc gattgaaaaa ctcggcaatc tctttctcta
     7441 acgccaggtg cggcgcataa ctgccgtttg ccatacgaga accggtggtg ccggtgcctt
     7501 gggccgctaa cgctgcctga ccttcggcga tggcatcatg attaaaggtt aaaccgaggt
     7561 aattattggt cccggccaga atcacttttt tattgccgat ccggccttcg gtggcggaat
     7621 acacttcatc aatacaggtg ccaaacggtg ttaacccgtt gtcactaaat tgcagtcgct
     7681 cgccagcaag gcgcgcatat ttatcgtata gccccattat ttattctcca gccatggaag
     7741 tagagtttcc attaattgcg caggggtttt gacatccagc agaatattaa taggaatgga
     7801 aatatcgaat ctgtcttcca gcatcattaa gagatccatc actttgatcg actccaggcc
     7861 aagatcgttg acgagatcgc tgtcgggctt aatttccaca ccgttttcga ccaaatcctg
     7921 taaacaggaa aggatgtaat ccattactat ttcacgattt accatagaaa cgttaacaca
     7981 cctttaaaca agacaatcaa aatagcccgt tgcgtaatgc gtgttccagg ctaatcccgg
     8041 gaaaccaatt aatatcttca gaaatacggt tattacttgc cgaccagtcg gcgtgggtta
     8101 attcacgaat tttcgagcgg gtcagcatag gttctttacc tgccaggcga ctcaacgcgg
     8161 tactgatatc cgcaaggcag gtgagtaccg gcagaggaat gccaaccatc cgcacagaac
     8221 cacaacgggc ttcggcggca agttgctgta cgcgttgcca gtcatagccg ccagcgatgc
     8281 catcgcataa ttcataggtt tgcgtctgta cagtttcggc gcttaaccac tgacccactg
     8341 cttgcgcaaa atcggtgaca tgcaggaaag agagctgtgt atctggtgca ccaagtcgtg
     8401 gcagcaggcc gcgcagcatc cagtcaaaca gcggttttaa ctctttatcg ccgggaccat
     8461 acacggctgt cgggcgaaaa acgcccagcg taatttcgtc agccattgcc gtcagccgtt
     8521 gttcggcgac gtgtttggaa tttgcgtacc aggagagctc gggatggcgc gccgccagcg
     8581 aagagataaa cagaaaacgt tggcaaaagc cgctctcttt tgctgcttgc atcaggcgca
     8641 ggctgccgtc aacgttacag tgggtaaaaa tctcttcttt gtgtccgcgc acttgcccgg
     8701 cgcaatggac caccgcgctg gctccggcaa ccagttcgct aagcgaatgt gtatcttcca
     8761 gcgaaccgcg cacccaggtg agattgtcat tgacgtgagc gcgggcagta cgcgtcaatg
     8821 cgcgaacgtg aaagccgcgg gcgagcaggt tatcgataat atatttaccg ataaacccgg
     8881 tagcgcccgt caccgcgaca gtttgattca tgccagggat tcctgcacat gaagactggc
     8941 agcataagcc ttctgataac gttttttcgc ttccgcacgg gcaggcttgc cggaggacgt
     9001 tcggggaata ctgtggggcg gcaacagatc gatagccgcg gtcacgccaa attcgctttg
     9061 gatccgtgct gccagcgcgt ggataagctg cccgcgacgt tcttcgtcgc tgatccgaca
     9121 ctggatctgc aaaatgattt tttcctgggc ggtaacaaaa gcaatcgcat cgccagaatg
     9181 aatttccggt tcctgttccg ctatatattc aatatcctgc ggccagatat tacggccacg
     9241 aataataatc agatctttaa tgcgtccggt gacatacaga taaccgtcca gcagataacc
     9301 gaggtcgccg gtgtctaacc agcccgtcgc ggcaatctcg tcttgcgaag cctggtcgcc
     9361 aaagtaaccg ctcatcagac tgggaccgga gatgcaaata tggcctacca cacgttccgc
     9421 gaccggcata cctgcttcat tgcggatttc aatgccatgt tctggcaacg ctttgccgca
     9481 gttgacgaaa gtcgatacgg cgcgtgtctc tgcacccggc gcgacggctt taccctgata
     9541 ttcgaggatg tcgcgatcca cttcgttaac cacaaccccg gaggcttcat cagagaagct
     9601 gacagccagc gcattttctg ccagtccgta gcacggcatg aaagttttat tgtcaaagtt
     9661 aacctgacgg aaacattcag cgaattgatg gagttgttct gcggagatgg gttctgcacc
     9721 aataccagcg acgcgccagc aggaaagatc cagttcagcg agatcttttt cattcacgcg
     9781 gcgctggcac aattcatagc caaacggcgg cgcaacggaa acggtgccgc gatttttact
     9841 gatcaattta agccattgca gaggacgcat ggcaaaatcc tgagtgcgca aataatctac
     9901 tgaaagttgc gtggcgacgg gggtcaggag aaagcctacc agtcccatat catggtagaa
     9961 aggcagccag gagacgcagc ggtcgccagg gcgtaattta atgccgtcgt ggcttatagc
    10021 acgtagatta gccatcactt cgcgatgggt gataatgacg ccacggggaa aacgggtgct
    10081 gccggaggtg tactggaggt aggcgatatc gttcggaact ggacgctgga gcgcaacatc
    10141 ggcttccggt aatgccttaa accaggtgtg gcttaataca tgcaattcgg ggttgttatg
    10201 cgtggcggca ttgaccagcg gcaaccactc atcaccagtg ataatggcag cgggctggca
    10261 acttgccagt aaaccctgca gtttggcgct ccaggaatcc cgctgaccga cgcccattgg
    10321 aatcgccaac gggacggcga ctaagccggc atactggcag gcaaaaaaag cctctacgaa
    10381 cccgctactg gtttcggcaa tcagcgccac gcgatcgcct tttttcaggt tcagcgataa
    10441 caaccgcttc gcaccagctt cggcacgtgc ttttaacgtc tgatattcca gttgatcttc
    10501 aagttggcaa cgtctgtcat aaaaattcat tccagcgcta ctcagagcgg cgtagtccaa
    10561 agcatcaacc agcgttggaa aatcggcata gcgcataggt agggaatgcg taaagatttt
    10621 attagacata tacaccacga aaaaattgga tatcaaaaat aa
//
LOCUS       DAZKFU020000164        10627 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZKFU020000164 DAZKFU020000000
VERSION     DAZKFU020000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.165"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..94)
                     /gene="yojO"
                     /locus_tag="ACHOQQ_RS16865"
                     /old_locus_tag="pgaptmp_003373"
     CDS             complement(<1..94)
                     /gene="yojO"
                     /locus_tag="ACHOQQ_RS16865"
                     /old_locus_tag="pgaptmp_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HGU4471585.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNG"
     gene            203..697
                     /gene="napF"
                     /locus_tag="ACHOQQ_RS16870"
                     /old_locus_tag="pgaptmp_003374"
     CDS             203..697
                     /gene="napF"
                     /locus_tag="ACHOQQ_RS16870"
                     /old_locus_tag="pgaptmp_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGU4471586.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            687..950
                     /gene="napD"
                     /locus_tag="ACHOQQ_RS16875"
                     /old_locus_tag="pgaptmp_003375"
     CDS             687..950
                     /gene="napD"
                     /locus_tag="ACHOQQ_RS16875"
                     /old_locus_tag="pgaptmp_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGU4471587.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNASPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            947..3433
                     /gene="napA"
                     /locus_tag="ACHOQQ_RS16880"
                     /old_locus_tag="pgaptmp_003376"
     CDS             947..3433
                     /gene="napA"
                     /locus_tag="ACHOQQ_RS16880"
                     /old_locus_tag="pgaptmp_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HGU4471588.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3440..4135
                     /gene="napG"
                     /locus_tag="ACHOQQ_RS16885"
                     /old_locus_tag="pgaptmp_003377"
     CDS             3440..4135
                     /gene="napG"
                     /locus_tag="ACHOQQ_RS16885"
                     /old_locus_tag="pgaptmp_003377"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGU4471589.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4122..4985
                     /gene="napH"
                     /locus_tag="ACHOQQ_RS16890"
                     /old_locus_tag="pgaptmp_003378"
     CDS             4122..4985
                     /gene="napH"
                     /locus_tag="ACHOQQ_RS16890"
                     /old_locus_tag="pgaptmp_003378"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGU4471590.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4982..5431
                     /gene="napB"
                     /locus_tag="ACHOQQ_RS16895"
                     /old_locus_tag="pgaptmp_003379"
     CDS             4982..5431
                     /gene="napB"
                     /locus_tag="ACHOQQ_RS16895"
                     /old_locus_tag="pgaptmp_003379"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGU4471591.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5441..6043
                     /gene="napC"
                     /locus_tag="ACHOQQ_RS16900"
                     /old_locus_tag="pgaptmp_003380"
     CDS             5441..6043
                     /gene="napC"
                     /locus_tag="ACHOQQ_RS16900"
                     /old_locus_tag="pgaptmp_003380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGU4471592.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6056..6679
                     /gene="ccmA"
                     /locus_tag="ACHOQQ_RS16905"
                     /old_locus_tag="pgaptmp_003381"
     CDS             6056..6679
                     /gene="ccmA"
                     /locus_tag="ACHOQQ_RS16905"
                     /old_locus_tag="pgaptmp_003381"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGU4471593.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            6676..7338
                     /gene="ccmB"
                     /locus_tag="ACHOQQ_RS16910"
                     /old_locus_tag="pgaptmp_003382"
     CDS             6676..7338
                     /gene="ccmB"
                     /locus_tag="ACHOQQ_RS16910"
                     /old_locus_tag="pgaptmp_003382"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGU4471594.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7380..8117
                     /gene="ccmC"
                     /locus_tag="ACHOQQ_RS16915"
                     /old_locus_tag="pgaptmp_003383"
     CDS             7380..8117
                     /gene="ccmC"
                     /locus_tag="ACHOQQ_RS16915"
                     /old_locus_tag="pgaptmp_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGU4471595.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8114..8323
                     /gene="ccmD"
                     /locus_tag="ACHOQQ_RS16920"
                     /old_locus_tag="pgaptmp_003384"
     CDS             8114..8323
                     /gene="ccmD"
                     /locus_tag="ACHOQQ_RS16920"
                     /old_locus_tag="pgaptmp_003384"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGU4471596.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRSVAQQRAREARLRAAQQQEAA"
     gene            8320..8799
                     /gene="ccmE"
                     /locus_tag="ACHOQQ_RS16925"
                     /old_locus_tag="pgaptmp_003385"
     CDS             8320..8799
                     /gene="ccmE"
                     /locus_tag="ACHOQQ_RS16925"
                     /old_locus_tag="pgaptmp_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGU4471597.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8796..>10627
                     /gene="ccmF"
                     /locus_tag="ACHOQQ_RS16930"
                     /old_locus_tag="pgaptmp_003386"
     CDS             8796..>10627
                     /gene="ccmF"
                     /locus_tag="ACHOQQ_RS16930"
                     /old_locus_tag="pgaptmp_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGU4471598.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVR"
BASE COUNT         2170 a         2731 c         3194 g         2532 t
ORIGIN      
        1 aaccatttga aatgtgagca aaagcccgtt tttgccacgc ttcccgccac tcttttgatc
       61 ctgctacagg ttttacctcg atcggggtat gcatctttga cacatccttt aatatcttag
      121 cggctataaa aatggcttat taattatgcg gtttatttgg tcgctctcaa ttttcagagc
      181 gcgttaatga tggaaggtca atgtgaagat tgatgcatcc cgtcggggca tactcactgg
      241 tcgctggcgc aaagccagta acggtatccg tccgccctgg tccggtgatg aatctcattt
      301 tctgacccat tgcacccgct gcgacgcctg tattaatgct tgtgaaaaca acattctgca
      361 acgcggcgcg ggcggctatc cgagcgttaa tttcaaaaat aacgagtgca gcttctgtta
      421 cgcctgcgcg caggcttgcc ccgaatcgct attttctccg cgccacacca gggcctggga
      481 tttgcagttc accattgggg acgcgtgcct ggcgtatcag tcagttgaat gccgccgctg
      541 tcaggatagc tgtgagccaa tggcaattat ctttcgcccg acgctctccg ggatctacca
      601 gccgcaactt aatagccaac tttgcaacgg atgtggagca tgtgcagcca gctgtccggt
      661 atcagccata actgcggagt atctccatgc acactaactg gcaagtttgc agcctggtcg
      721 tgcaggccaa aagcgaacga atttcagaca tcagcaccca actgaacgcc tctcccggct
      781 gtgaagttgc tgtcagcgac gcgccgagcg gtcagttgat tgtggtggtg gaagcagaag
      841 acagcgaaac gctgatccaa accattgagt cagtacgcaa cgtagagggc gtgctggcgg
      901 tgtcgctggt ttatcaccag caggaagagc aaggtgagga aacaccatga aactcagtcg
      961 tcgtagcttt atgaaagcta acgccgttgc ggccgctgcg gcggctgccg gtctcagcgt
     1021 gccgggcgtt gcccgcgccg ttgttggtca gcaggaagcc atcaaatggg ataaagcgcc
     1081 gtgccgtttc tgcggtactg gttgcggcgt tctggtcgga acgcagcagg ggcgtgtggt
     1141 ggcctgtcag ggcgacccgg acgcaccggt taaccgtggc ctgaactgca ttaagggcta
     1201 tttcctgccc aagatcatgt acggtaaaga ccgtttgacg cagccgctgc tgcgtatgaa
     1261 aaacggtaaa tatgacaaag aaggcgaatt taccccaatc acctgggatc aggccttcga
     1321 tgtgatggaa gagaaattca aaaccgccct gaaagaaaaa gggccggaat cgatcggtat
     1381 gttcggttct ggtcagtgga ctatctggga aggttatgcc gcatccaagc tgtttaaagc
     1441 gggcttccgt tcgaacaaca tcgacccgaa cgcgcgtcac tgtatggcgt cggcagtagt
     1501 tggctttatg cgtacctttg gtatggatga gccgatgggc tgctatgacg acatcgagca
     1561 ggctgacgcg tttgtgctgt ggggcgcaaa catggcggag atgcacccga tcctctggtc
     1621 acgcatcact aaccgtcgtc tctctaacca ggacgtcacc gtggcggtgc tttcaaccta
     1681 ccagcatcgt agcttcgagc tggcggataa cggcatcatc tttacgccgc aatctgacct
     1741 ggtgatcctg aactacatcg ccaactatat cattcaaaac aatgcgataa atcaggactt
     1801 ctttagcaag cacgttaacc tgcgcaaagg ggcgacggac atcggctacg gtttacgtcc
     1861 gacccatccg ctggaaaaag cagcgaagaa tccgggttct gacgcctccg aaccgatgag
     1921 ctttgaagat tacaaagcct tcgttgctga gtatacgctg gaaaaaaccg ctgaaatgac
     1981 tggcgtacca aaagatcagc tggaacaact ggcgcagctg tatgccgatc cgaacaagaa
     2041 agtcatctcc tactggacga tgggcttcaa ccagcatact cgtggcgtgt gggccaacaa
     2101 cctggtctac aacctgcacc tgctgaccgg caaaatttcc cagccgggtt gcggtccgtt
     2161 ctccctgacc gggcagcctt ccgcgtgtgg tactgcgcgt gaagtgggca cctttgctca
     2221 ccgtctgcct gcggacatgg tggtaactaa cgagaaacat cgtgatatct gcgagaagaa
     2281 gtggaatatc ccgagcggca ccattccggc gaaaatcggt ctgcatgcgg tagcacaaga
     2341 ccgtgcgctg aaagacggca agctgaatgt ttactggacc atgtgtacca acaacatgca
     2401 ggccgggccg aacattaatg aagagcgtat gccgggctgg cgcgatccgc gcaacttcat
     2461 catcgtctcc gatccgtatc cgacagtcag tgcgctggcc gccgacttga tcctgccgac
     2521 cgcaatgtgg gtagaaaaag agggggctta cggtaacgcc gaacgccgta ctcagttctg
     2581 gcgtcagcag gtacaggcgc caggcgaagc gaaatcggac ctctggcagt tagtccagtt
     2641 ctcccgccgc ttcaaaactg aagaagtatg gccggaagag ctgctggcga agaaaccgga
     2701 actgcgtggc aaaacgctgt acgaagttct gtatgccacc cccgaagtga gcaaattccc
     2761 ggtatccgaa ctggcggaag atcagctgaa cgatgaatcc cgcgagctgg gcttctatct
     2821 gcaaaaaggg ctgttcgaag agtacgcatg gtttggtcgc ggtcacggtc acgatctcgc
     2881 gccgttcgat gactaccaca aagcgcgcgg tctgcgctgg ccggtggtga acggtaaaga
     2941 aacgcagtgg cgttacagcg aaggtaacga cccgtacgtg aaagcgggcg aaggctacaa
     3001 gttctacggt aaaccggatg gcaaagcggt gatcttcgca ctgccgttcg aaccagcggc
     3061 ggaagcaccg gatgaagagt acgacctgtg gctctctacc ggacgcgtcc tggagcactg
     3121 gcacaccggc agtatgactc gccgtgtgcc ggaactgcac cgtgccttcc cggaagcggt
     3181 cctgtttatt cacccgctgg atgcgaaagc gcgcgatctg cgccgtggcg acaaagtgaa
     3241 agtggtttct cgccgtggcg aagtgatctc gattgtcgaa acgcgcggtc gtaaccgtcc
     3301 gccgcagggc ctggtgtaca tgccgttctt cgacgccgca cagctggtta acaaactaac
     3361 gctggacgcg accgatccgc tctcgaaaga gacggacttc aagaagtgcg cggtcaaact
     3421 ggagaaggtg taagccatta tgtcccggtc agcgaaacct caaaatggtc gccgccgctt
     3481 tctgcgcgat gtcgttcgca cagcaggcgg gctggctgcc gtgggtgtgg cgctggggtt
     3541 acaacagcaa accgcacgcg catctggcgt gcggttgcgc ccgcccggag ccataaacga
     3601 gaacgccttt gccagtgctt gtgtgcgttg cggtcagtgt gttcaggctt gcccttacga
     3661 caccttaaaa ctggcgacgc tggcctctgg cctgtcggcg ggcacgccat attttgtcgc
     3721 acgggatatt ccttgcgaaa tgtgtgagga cattccgtgc gccaaagtgt gcccgagcgg
     3781 tgcgctggat cgtgagattg aatcgatcga cgacgcgcgg atggggctgg cggtactggt
     3841 ggaccaggaa aactgtctca actttcaggg gctgcgctgc gatgtttgtt atcgcgaatg
     3901 cccgaaaatt gatgaggcca tcaccctgga gctggagcgc aacacgcgta ccggtaagca
     3961 cgcccgcttt ctgccgacgg ttcacagcga cgcctgtacc ggttgcggta agtgcgaaaa
     4021 agtgtgcgtg ctggaacaac cggcaatcaa ggtgttaccg ctgtcactgg cgaaagggga
     4081 gttaggccac cattaccgct tcggctggct ggaggggaac aatggcaaat cgtaaacgtg
     4141 acgccgggcg cgaggcgctg gagaaaaaag gctggtggcg cagtcaccgt tggctggtgt
     4201 tacgtcgcct ttgtcagttc ttcgtgctgg ggatgttttt gagtggtccg tggtttggtg
     4261 tgtggatctt gcacggcaac tacagcagta gcctgttatt cgacaccgtt ccgctgaccg
     4321 atccgctgat gacgctgcaa agtcttgcca gtggtcatct gcctgccacg gtggcgttga
     4381 ccggtgcggt gattatcacc gtgctctacg ccctggcagg aaagcgatta ttttgcagct
     4441 gggtctgccc gctgaacccg attaccgact tagcgaactg gttacgcagg cgctttgacc
     4501 tcaatcagtc tgcgacgatc cctcgccaca ttcgctacgt gctgttggtg gtcattctgg
     4561 tgggatcagc cttaactggc acgctcatct gggaatggat caaccccgtt tccctgatgg
     4621 ggcgtagcct ggtaatgggc ttcggcagcg gcgcgctgct tattctcgcg ctgtttttat
     4681 ttgatctact ggtcgttgaa cacggctggt gcgggcacat ttgtccggtt ggcgcgctgt
     4741 atggcgtgct gggtagcaaa ggtgtgatta ccgttgcggc cagcgatcgg cagaaatgta
     4801 accgctgtat ggattgtttt catgtttgcc cggaaccgca tgtgctacgt gccccggtgc
     4861 tggatgaaca aagcccggtg caggtcacca gccgcgattg catgacgtgc ggtcgctgcg
     4921 tggatgtctg ttctgaggat gtatttacaa taactacacg atggagttcg ggagcgaaat
     4981 catgaaaagc catgacctga agaaagcgct gtgtcaatgg acggcgatgc tggccctggt
     5041 ggtaagcggc gcggtttggg cggctaatgg cgtcgatttt agccagtcgc cggaagtatc
     5101 cgggacgcag gaaggggcca ttcggatgcc aaaagagcag gatcggatgc cgctgaacta
     5161 tgtgaatcag ccgccaatga tcccgcatag cgttgaaggt tatcaggtaa cgaccaatac
     5221 caaccgctgc ttgcagtgcc acggtgtcga aagctatcgc accactggcg cgccgcgtat
     5281 cagtcctact cactttatgg acagcgacgg caaagtgggc gcggaagtgg ctccgcgtcg
     5341 ttatttctgt ctgcaatgtc acgtaccgca ggccgatacc gcgccaatcg tggggaatac
     5401 ctttacccca tcaaaaggtt acgggaaata agaggttatt atgggaaatt ctgaccgtaa
     5461 gcctggtctg attaagcgcc tgtggaaatg gtggcgtacc cccagccgtc tggcgctggg
     5521 gacgctgctg ttgatcggtt ttgtcggcgg catcgtcttc tggggcggct ttaacaccgg
     5581 gatggaaaaa gccaataccg aagagttctg cattagctgc cacgaaatgc gtaacacggt
     5641 gtatcaggaa tacatggatt ccgtgcacta caacaaccgt agcggcgtcc gtgcgacctg
     5701 tccggattgt cacgttccgc acgagtttgt gccgaagatg atacgcaagc tcaaagcaag
     5761 taaagagctg tatggtaaaa tttttggcgt tattgacacg ccgcagaaat ttgaagctca
     5821 tcgtctgacg atggcacaga atgagtggcg gcgcatgaag gacaataact cgcaggagtg
     5881 ccgtaactgt cacaacttcg agtatatgga tacaaccgcc cagaaatcgg ttgccgcgaa
     5941 gatgcatgac caggcggtga aagatgggca aacctgtatt gattgccata aagggatagc
     6001 gcacaagctc cccgatatgc gtgaagtcga gccaggtttt taacagggtt attgcgtggg
     6061 tatgcttgaa gtcagagagt tactttgtga gcgggatgaa cgaaccttat ttagtggctt
     6121 gtcatttacg ctgaacgcag gagagtgggt acaaatcacc ggtagcaacg gcgcggggaa
     6181 gacaacgctt ctccgtttgc tgacggggtt gtctcgccct gacgcaggcg aggttctctg
     6241 gcaagggcag cccttgcatc aggtacgcga cagctaccat caaaacctgt tatggatagg
     6301 ccatcagccg gggatcaaaa cccggctgac ggcgttagaa aatctgcact tttatcatcg
     6361 cgatggcgat accgcacaat gtctggaagc cctggcgcag gccgggcttg ccggattcga
     6421 agatattccg gtaaatcaac tttctgccgg gcaacaacgc cgcgtcgctt tagcgcgtct
     6481 gtggctgacc cgtgccacgt tatggatcct cgacgagcct tttaccgcga ttgacgttaa
     6541 cggcgtcgat cgtctgaccc agcgtatggc gcagcataca gagcaggggg ggattgtgat
     6601 tctcactacc caccagccgc tcaacgttgc tgaaagtaaa attcgccgca tttcactgac
     6661 gcaaacgagg gccgtatgat gttctggcgc attttccgtc ttgagctgcg cgtagcgttt
     6721 cgccatagcg ctgaaatcgc caacccgctg tggttcttcc tgattgtaat tacccttttt
     6781 ccgctcagta tcggtccgga accgcaactg ctggcacgta ttgcaccggg cattatctgg
     6841 gttgccgcgc tgctttcatc cctgctggcg ctggaacgac tgttccgtga cgatttgcag
     6901 gacggcagtc ttgaacaatt gatgttgttg ccgttaccct tgcccgccgt tgtgctggcg
     6961 aaggtgatgg cgcactggat ggtaaccggt ctgccgttac tcatcctttc gcccctggta
     7021 gcaatgctac tgggaatgga tgtttatggc tggcaagtga tggcgttaac gctactgctg
     7081 ggaaccccta cgcttggctt tcttggtgcg ccgggcgtgg cgctgacagt gggacttaag
     7141 cgcggtggtg tgctgctcag catactggtg ttaccgctga ctatcccatt actcatcttt
     7201 gccaccgccg cgatggacgc ggcttctatg catttgcccg ttgacgggta tctggcaatt
     7261 ttaggcgcgt tgctggcagg caccgcgaca ttaagtcctt ttgcgacggc ggcagcgtta
     7321 cgaatcagca ttcaataacg ctggattact gatttttgtt cgagtctggt atcgaaacta
     7381 tgtggaaaac actgcatcaa ctggcgatcc caccacggct gtatcaaatc tgtggctggt
     7441 ttataccgtg gctggcaatt gccagtgtgg tcgtgcttac cgtcggctgg atctggggat
     7501 tcggctttgc tccggctgat tatcagcagg gaaatagcta ccgcattatc tacctgcatg
     7561 tgcctgcggc gatctggtcg atgggcattt atgcatcaat ggcggtggcg gcttttattg
     7621 gccttgtctg gcagatgaaa atggccaacc tggcggtggc ggcgatggcc cccattggtg
     7681 ccgtgtttac ctttattgcc ctggttaccg gctctgcatg ggggaaaccg atgtggggca
     7741 cctggtgggt atgggatgca cgtctgactt ctgaactggt gctgctgttt ttgtatgtgg
     7801 gtgtgattgc cctgtggcac gccttcgacg accgccgtct ggcgggccgt gcggcaggta
     7861 tcctggtgct gattggcgtg gtgaatctgc cgattattca ttactccgtg gagtggtgga
     7921 acaccctgca tcagggatca acgcggatgc agcaaagtat cgatcctgcg atgcgttcgc
     7981 cgctgcgctg gtcgattttt ggcttcctgc tgctgtctgc cacgctgacg ctgatgcgga
     8041 tgcgtaattt gattttgctg atggaaaaac gccgtccgtg ggtgagtgaa ctgatactga
     8101 aaagaggccg taaatgaccc ctgcatttgc ttcctggaat gaatttttcg caatgggcgg
     8161 ttacgccttt tttgtctggc tggcggtggt gatgaccgtt attccgctgg tggttttggt
     8221 cgtgcactcg gtgatgcagc atcgcgcaat tctgcgtagc gtggcgcaac agcgggcgcg
     8281 agaggcgcgt ttacgtgctg cacaacagca ggaggctgca tgaatattcg ccgtaaaaac
     8341 cgcttgtgga ttgcctgtgc cgtgttggca gggctggcgc tgactatcgg tctggtgcta
     8401 tatgcgctgc gctcgaatat cgatctcttt tatacgccgg gggaaattct ctacggcaag
     8461 cgtgaaactc agcagatgcc ggaagtcggt cagcgtctgc gcgttggcgg gatggtgatg
     8521 ccgggtagcg tgcagcgcga tcccaattcg ctgaaagtaa cgttcaccat ttacgatgct
     8581 gaaggctcag tggatgtctc ttacgaaggc attttgccgg atctgttccg tgaagggcag
     8641 ggcgttgtgg tgcagggcga gctggaaaaa ggcaatcata tcctcgcgaa agaagtgctg
     8701 gcgaaacacg acgaaaacta cacgccgcca gaagttgaga aagcgatgga agctaaccac
     8761 cgtcgcccgg cgagtgttta taaggaccca gcatcatgat gccggaaatt ggtaacgggc
     8821 tgctgtgcct ggcgctagga attgcgctgc tgctgtctgt gtatccgcta tggggcgtgg
     8881 cgcgcggaga tgcgcgcatg atggcgtctt cccgcttgtt tgcctggctg ctgtttatgt
     8941 ctgtagctgg cgcatttctg gtgctggtca atgctttcgt ggtcaacgac ttcaccgtca
     9001 cctatgttgc cagcaactcc aatacccagc ttccggtgtg gtatcgcgtg gcggctacct
     9061 ggggtgcaca tgaaggctca ctgctgctgt gggtgctgct gatgagcggc tggacctttg
     9121 cggtggcgat ttttagtcag cgtattccgc tggatattgt ggcccgcgta ctggcgataa
     9181 tggggatggt cagcgtcggc tttttgctgt tcattctctt tacctctaac ccattctcac
     9241 gcacgctgcc gaacttcccg attgaagggc gcgatcttaa cccgctgttg caggatccgg
     9301 ggctgatctt ccatccgcct ctgctctata tggggtacgt gggtttctcg gtggcgtttg
     9361 cttttgccat tgcttctttg ttgagcgggc gtctggacag cacttatgcg cgttttactc
     9421 gtccgtggac gctggcagcg tggatcttcc tgacgctcgg catcgtgctc ggttccgcat
     9481 gggcctatta cgaactcggc tggggtggct ggtggttctg ggatccggta gaaaacgcct
     9541 cgtttatgcc gtggctggtg gggactgcgt tgatgcactc gctggcggtg actgaacagc
     9601 gtgccagctt caaagcgtgg acattactgc tggcaatcag tgccttctcg ttgtgtctgc
     9661 tggggacttt cctcgtgcgt tccggcgtgc tggtatcggt acacgcgttt gcgtctgatc
     9721 cggcgcgcgg tatgtttatc ctcgccttta tggtgctggt gattggcggt tcgctgctgc
     9781 tgtttgccgc gcgtggacac aaagttcgct cacgcgtaaa caatgcgctg tggtcgcggg
     9841 aatctttgct gttagcgaac aacgttttgc tggtcgccgc gatgctggtg gtgttgctgg
     9901 ggacgctgct gccgctggtg cacaaacaac tggggctggg cagtatttcg attggcgaac
     9961 cgttcttcaa caccatgttt acctggctga tggtgccgtt tgcgctactg cttggtgtcg
    10021 gtcctctggt gcgctggggg cgcgatcgcc cacgtaaaat ccgcaattta ttgattatcg
    10081 ccttcatctc tacgctggtg ctgtctctgc tgttgccgtg gctgttcgaa agcaaagttg
    10141 tggcgatgac ggtgctcggc ctggcaatgg cctgctggat tgcggtgctg gcaattgcag
    10201 aagcggcgct gcgtatttca cgcggcacga aaaccacctt cagctattgg gggatggtgg
    10261 cggctcacct ggggctggca gtgacaattg ttggtattgc tttcagtcag aactacagcg
    10321 ttgagcgtga tgtgcgcatg aagtccggcg atagcgtcga tattcatgaa tatcgcttca
    10381 ccttccgtga tgttaaagag gtgactggtc cgaactggcg cggcggtgtg gcgactatcg
    10441 gcgtaacgcg agatggcaag ccggaaacgg tgctgtatgc ggaaaaacgt tattacaaca
    10501 ctgccgggtc gatgatgacc gaagcggcga ttgacggcgg catcacccgt gacctgtacg
    10561 cggcactcgg tgaagaactg gaaaacggtg cctgggctgt tcgtctttac tacaaaccgt
    10621 ttgttcg
//
LOCUS       DAZKFU020000165        10560 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZKFU020000165 DAZKFU020000000
VERSION     DAZKFU020000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.166"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            86..994
                     /locus_tag="ACHOQQ_RS16935"
                     /old_locus_tag="pgaptmp_003387"
     CDS             86..994
                     /locus_tag="ACHOQQ_RS16935"
                     /old_locus_tag="pgaptmp_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HGU4471599.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVLLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1214..3205)
                     /gene="tkt"
                     /locus_tag="ACHOQQ_RS16940"
                     /old_locus_tag="pgaptmp_003388"
     CDS             complement(1214..3205)
                     /gene="tkt"
                     /locus_tag="ACHOQQ_RS16940"
                     /old_locus_tag="pgaptmp_003388"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4471600.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3483..4241
                     /gene="loiP"
                     /locus_tag="ACHOQQ_RS16945"
                     /old_locus_tag="pgaptmp_003389"
                     /gene_synonym="yggG"
     CDS             3483..4241
                     /gene="loiP"
                     /locus_tag="ACHOQQ_RS16945"
                     /old_locus_tag="pgaptmp_003389"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGU4471601.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4447..5367)
                     /gene="speB"
                     /locus_tag="ACHOQQ_RS16950"
                     /old_locus_tag="pgaptmp_003390"
     CDS             complement(4447..5367)
                     /gene="speB"
                     /locus_tag="ACHOQQ_RS16950"
                     /old_locus_tag="pgaptmp_003390"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGU4471602.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5503..6234)
                     /locus_tag="ACHOQQ_RS16955"
                     /old_locus_tag="pgaptmp_003391"
     CDS             complement(5503..6234)
                     /locus_tag="ACHOQQ_RS16955"
                     /old_locus_tag="pgaptmp_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471603.1"
                     /translation="MKKWKVRSALIALVVLLVGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRA
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6380..8356)
                     /gene="speA"
                     /locus_tag="ACHOQQ_RS16960"
                     /old_locus_tag="pgaptmp_003392"
     CDS             complement(6380..8356)
                     /gene="speA"
                     /locus_tag="ACHOQQ_RS16960"
                     /old_locus_tag="pgaptmp_003392"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGU4471604.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8365..8496)
                     /gene="yqgB"
                     /locus_tag="ACHOQQ_RS16965"
                     /old_locus_tag="pgaptmp_003393"
     CDS             complement(8365..8496)
                     /gene="yqgB"
                     /locus_tag="ACHOQQ_RS16965"
                     /old_locus_tag="pgaptmp_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGU4471605.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8590..8733)
                     /gene="yqgG"
                     /locus_tag="ACHOQQ_RS16970"
                     /old_locus_tag="pgaptmp_003394"
     CDS             complement(8590..8733)
                     /gene="yqgG"
                     /locus_tag="ACHOQQ_RS16970"
                     /old_locus_tag="pgaptmp_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGU4471606.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8844..9095)
                     /gene="yqgD"
                     /locus_tag="ACHOQQ_RS16975"
                     /old_locus_tag="pgaptmp_003395"
     CDS             complement(8844..9095)
                     /gene="yqgD"
                     /locus_tag="ACHOQQ_RS16975"
                     /old_locus_tag="pgaptmp_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGU4471607.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            9151..10305
                     /gene="metK"
                     /locus_tag="ACHOQQ_RS16980"
                     /old_locus_tag="pgaptmp_003396"
     CDS             9151..10305
                     /gene="metK"
                     /locus_tag="ACHOQQ_RS16980"
                     /old_locus_tag="pgaptmp_003396"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGU4471608.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2627 a         2816 c         2676 g         2441 t
ORIGIN      
        1 ttatagaatc tgaattctca cctgtcggct gttttctcct catctcatgt gacatattcc
       61 cacactttac tcaaggaaga ttgtcatgaa agcacctgtt tcattaacct catttcgccc
      121 acaaaaatct cttgctatcg ccatcggcgt gcttgccgtg gttttgctgc catttctctc
      181 gtattacacc gttaatgaag gtgaacgcgg cattttgctg cgctacggaa aaatcgtaaa
      241 agttgccgag ccgggtctgg gttttaagat cccgtttatg gaaagcgtgg agaaaatttc
      301 cacgcgtaat caggctgtgg tctatcaggg attacaggcc tacagccgcg accagcaacc
      361 ggcacaaatg acagtatcag tcagcttcca cattaaacct tcagaagcag gcgcggttta
      421 taccacctac aacaccattg aagcattaaa agatcgtctg attgtccgtc agttgccaac
      481 tcaactggag aacgttttcg gccaatatac cgccatcagc gctgtacagg atcgtacaaa
      541 actggttcag gatctgcaaa atgccatgcg caaagccgtt gttgggccag tggtaattga
      601 cggggtccag attgaaaata ttgatttttc tgacgcttat gaaaagtcga ttgaagaccg
      661 aatgaaagca gaagtcgcca ttgccacgcg taagcaaaat ctggaaacag agaaaatcca
      721 ggcgcaaata gcggtaactc aggcccaggc agaagcagac agtaaactgg cggcagccaa
      781 agcagaagcg gaaactattc gcgtaagagg cgcggcagag gctgaaacta ttcgtttaaa
      841 aagtgcggct gaagcagaag caatccgcct gcgcggtgaa gctctgcggg ataatcccgg
      901 acttgtagcc ctcaccactg ccgaacgttg ggacggtaaa ctgcccgata ccatgatccc
      961 cggcagcaca gttccgttta tctctactaa gtaaaaaaag ggccgctttc gcggcccttg
     1021 agagtgatgg caaaagcaaa actgcctgat gcgctacgct tatcaggcct acatacttcc
     1081 tgcaatatat tgaatttgca cgatcttgta ggtcggataa ggcgttcacg ccgcatccga
     1141 catgaacaac gcgcacttta ccaataatct aaagggccgc tttcgcggcc cttctcacaa
     1201 aacgtcttac tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc
     1261 cgaactcttc aaacagcagc tctgccggag cagattcacc gaaggtggtc ataccgacga
     1321 tagcaccgtt caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa
     1381 cgcgtgcagt aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcga
     1441 atgcatcggt agacggcatg gaaaccacgc gcgccttcac gccttcggca gtcagttttt
     1501 cgtaggcggc aacagccagt tcaacttccg aaccggtcgc gatgaagatc agttccggct
     1561 gaccggcaca gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag
     1621 tacgctcctg ctgcgccagg ttctgacggg agaggataag cgcggtcggg ccgtcctgac
     1681 gctcaacacc gtatttccat gccactgcag attcaacctg gtcacatgga cgccatgtag
     1741 acatgttcgg ggtcacgcgc agagaagcaa cctgctctac cggctggtga gtcgggccat
     1801 cttcgcccag accgatggag tcgtgggtgt aaaccatcac ctgacgctgt ttcatcagcg
     1861 cagccatacg tacggcatta cgtgcgtatt ctacgaacat caggaaggtg gaggtgtacg
     1921 gcaggaagcc accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa
     1981 caccgtagtg gatgtagtta cccgcagcat cttcgttgat tgctttagaa ccagaccaca
     2041 gggtcaggtt agacggtgcc aggtcagcgg agccgccgag gaattccggc aacagcgggc
     2101 cgaacgcttc gatagcattc tgcgacgctt tacggctggc gattttcgcc gggttagcct
     2161 gcagtttagc aatgaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca
     2221 tacggcgggt aaattcagcg gcttcctgcg gataagcttt cgcgtaagct gcgaatttct
     2281 cgttccatgc ggattctttc gcctgacctg cttctttcgc atcccactga gcatagattt
     2341 cagacgggat ttcgaacggc gcgtatttcc agcccagctg ttcgcgggtc agggcaattt
     2401 cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga
     2461 aaccgatgat ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt
     2521 ctactgcgcg tttgatggat gctgcgtcat gaccgtcgat gtcgcgaata acgtgccagc
     2581 cgtaagcttc gaaacgcatt gcggtgtcgt cggtgaacca gccttcaacg tgaccatcaa
     2641 tagagatacc gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca
     2701 gagagcaaac ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg
     2761 tgtagtgatc gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg
     2821 caatcgccat acctactgcg ttggcaatac cctgccccag cggaccggtg gtggtttcca
     2881 caccagcggt gtaacccact tccgggtggc ccggagtttt agagtgcagc tgacggaagt
     2941 ttttcagttc ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca
     3001 tggagccgtg gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg
     3061 ggttgtgttt caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg
     3121 cacccgggtg accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat
     3181 tggcaagctc tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct
     3241 taacgacttg acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc
     3301 aagccgcgcg tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc
     3361 tcgcatgttg cgcaatctac tcgcccgtcc gctgcgcttt tccttatact gagactgagc
     3421 gtcggttcac ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagaaaaa
     3481 acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg actggttgcc
     3541 agaatatgga ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt
     3601 tgagtgatgc tcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg
     3661 cgacgattgc gccagccaat agcgaatacg ctaaacgtct gacaaccatt gccaacgcgc
     3721 taggcaacaa tatcaacggt cagccagtga attacaaagt gtatatggcg aaggatgtga
     3781 acgcctttgc gatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga
     3841 cggataacga agtcgaagcg gtgatcggtc acgaaatggg gcacgtggcg ttaggccatg
     3901 tgaagaaagg gatgcaggtg gcacttggta caaatgccgt gcgagtcgcg gcggcctctg
     3961 cgggcggtat cgtcggcagt ttatcgcaat cacagcttgg tgatctgggc gagaaattag
     4021 tcaattcgca attctcccag cgccaggaag cagaggcgga tgattactcc tacgatttac
     4081 tgcgccaacg cggcatcagt ccggcaggtc ttgccaccag ctttgaaaaa ctggcaaaac
     4141 tggaagaagg tcgccaaagc tcaatgtttg acgaccatcc agcatccgcc gaacgcgccc
     4201 agcatattcg cgatcgtatg agcgcggacg ggattaagta aggcatggtg gtgccaaacg
     4261 tagttcagat aaggcattta cgccgcatct gacataaatg gcacattcac tacctcgtgc
     4321 gcattgccgg atgcgatgct ggcgcatctt atccggccta cgggtgccaa acgtaggtca
     4381 gataaggcgt tcaccccgca tctgacatga atggcacgtt ttacccgtgc gcatcgcatc
     4441 tggtgcttac tcgccctttt tcgccgcctg aatatacagc atttccagcg ccagcgttgc
     4501 cgctgccaga gcagtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat
     4561 cccaacgatg ttgagatctt tcaggccgcg taccagttta atagcgcgat cggaggtcag
     4621 gccgccaatt accggggtac cggtgcccgg cgcaaatgca ggatccaggc agtcgatatc
     4681 aaaggtcagg taaaccggca tatcacccac aatttgtttc acctgggcga taacgtcatc
     4741 cacgctgcga tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa
     4801 ttcggtacga ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc
     4861 ggtatagaac atggtgccgt ggtcaaattc acaaccgttc gcgtaggtat cggtgtgggc
     4921 gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag
     4981 cgtaacaaag tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc
     5041 ggcgtgtgcc tgcagttttt cgctcatctc acgggcatcg ccgaaggcat ataccagatc
     5101 gccgcagtcc accacgttca gacgctcgcg catgtcgaaa ttccacggga agcggttgtg
     5161 ttcccaggcc aggttagtcg aaacctggcg aatcgccgcc gggccgtgac gaccaccggc
     5221 acggccagag gtggccatgt cgaacggcac gccggtgatc acccagtcgg catcgctgtc
     5281 atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag gcgttggaaa ccagtgagtt
     5341 atcgtattga tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa
     5401 aacccttccg cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt
     5461 gctaattatc gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg
     5521 cggcgcatag ccattgttat ctgctggaaa agcttgtcca gagcacggtg gcagcgcgtt
     5581 ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc
     5641 accctgcgcc gaattggctg cgtaggaatc aacaacgttg gcgaactgaa aagccgcttc
     5701 ggtatgttta tctttgcgga atcctttaat cgctaacgaa tctccggcat tcacaatata
     5761 gccgttgtta ttcagcgaac cttgcttgcc gttcagcaca tccaggccat caaccgtagc
     5821 aacgatctcg tagttggtgt tacgactgta attacgaaca tataattgat aattcattcc
     5881 gactctggcg tggacaatat agcgtggttc attattggat gaatcataag cccgcgtaat
     5941 aggtagagaa ttaaaattac catcgcgaat agcatattcc agttcgttaa tacgaatgct
     6001 ataaacgcgg tcgtagccgg aaggaacatt agtcgaataa ctaataacga tcatctctgc
     6061 aggatcgcgc caggctcgtt cagcagatac gccctgaacc gatgagtgca catccccacc
     6121 ccaggcggtg ccgacattgc ccgatgcact gctattatac tgcgcattac tggaacaacc
     6181 gaccagcagc acgactaatg ctatcaacgc gctacgtact ttccattttt tcataaccgt
     6241 tccctctgca tttaattaat gcagcgattg tagggagatt atttaataag aaaagcagct
     6301 aaattatttt taagaatata taaaaagaaa gcgggccgat ttacgtaaat aaatcgaccc
     6361 gccagcggaa taatcttaat tattcatctt caagataggt ataaccgtac aaacctgcct
     6421 cgaactcttc aaggaactgt tgttgcagtt cagcatcaag atcggttttc ttcacttgat
     6481 cacggaactg agttagcagc gttttcggat cgagctgtac atattgcagc atgtccgcca
     6541 cggtatcgcc ttcgtcagac agttccactt ctacactacc gtcagggaag acgaacacgt
     6601 caaccgcttc ggtatcaccg aacaggttgt gcatgttgcc gaggatctcc tgatatgcgc
     6661 cgaccataaa gaaaccgagc atcggcggat tctctggatc gtactccggc ataggcattg
     6721 tcgtggcaat accatcacca tcgatatagt ggtcgatagc accgtcagag tcacaggtaa
     6781 tatccagcag cacagcgcgg cgttccggca cttgatccag cccttccagc ggcagaaccg
     6841 ggaacaactg gtcgatcccc cacgcatccg gcatcgactg gaacagcgag aagttgacgt
     6901 acattttgtc cgccatacgt tcctgcagct cgtcgataat cggacggtga gcacggtttt
     6961 gcggatccag ctgcttttgc acttcatggc acatgctcaa ataaagctgc tcagcccatg
     7021 cacgttcttg caggctaaag gtgccggaag agtagccgat atgaatgtcg tgcagatcca
     7081 tctgactgtc gtgtaaccat tcacgcagag aacggcgagt tcccggttcg tgcatctcct
     7141 gccaggtttc ccacatgctt tgcagcgcgc gcggcgcatc ttctgcaggc gcggtcggca
     7201 ccgtgtattc gttacgttcc acgccgatga tattagacac cagcacggtg tgatgcgcag
     7261 tcactgcacg acccgattca gtgattaccg tcggatgcgg cagaccgttt tcttcacacg
     7321 catcaccaat cgcccagatg atgttgttgg cgtattcatt gaggccatag tttacggagc
     7381 agtcagactg cgaacgggtg ccttcatagt ccacgcccag accgccgccg acgtcgaagc
     7441 actgaatatt gacgcccagc ttgtgcagtt ccacatagaa acgcgcggat tcacgaacgc
     7501 ccgtggcgat atcgcgaata ttcgccatct gcgaaccgag gtggaagtgc agtagttgca
     7561 ggctgtcgag acgcccggct tcacgcaggg tttcaaccag ttgcagtacc tgagtcgcag
     7621 ccaggccgaa cttcgatttt tccccgccgg aagactgcca tttacccgag ccctgcgaag
     7681 ccagacgtgc acgcacgccc agacgaggaa cgacattcag acgttctgct tcatccagca
     7741 caatggcgat ttctgacatc ttctcaatga ccagatagac cttgtgcccc atcttctcgc
     7801 caattaatgc caggcggata tattcgcggt ctttataacc gttgcagacg atgacgctac
     7861 gggtcatgcc agcatgtgcc agcactgcca tcaactcggc tttggaaccg gcttccagac
     7921 ccagcggttc gcccgaatga atcagggact caatcacgcg acggtgctgg ttaactttga
     7981 tcggataaac aaggaagtaa tcgccgttat agccgtagga ttcccgcgca cgtttgaacg
     8041 cggcgttaat ggaacgcaaa cggtgctgca ggatctgtgg gaaacagaac agtgcaggca
     8101 gacgctggcc ctgtgcttca cgagttttca ctaactgcgc gagatcgacg cgagcttccg
     8161 ggacgtccgg gtccgggcac acgctgatgt ggcccagctc gttaacgtca tagtagttat
     8221 tgccccacca ggcaatattg taagtacgca gcatcttgct ggcttcctgg gagctcattg
     8281 caacctcctg catggagcgt agtacaccgt gttcgcccgc tgacgaaggc aaacccatag
     8341 acatgtcgtc agacatagcg aacctcaaat tattttatta agtgtaaaac agttaacgac
     8401 tatcgcagcc tggaactgcg ataacaaccc ataagcacat ggattttcca gcagtgaatg
     8461 ctgacgctcc aactgcgcaa ccggtttctt tttcataaca ttattaagca cataaccgaa
     8521 cgtaagtgtg acagttcggc gaaaccacga gaaaactctt gtttttacaa gagcgccctt
     8581 gttcagtcct cagtaactgt aaccagctct tgaatcctga gaagcgccga gatgggtata
     8641 acatcggcag gtatgcaaag cagagatgca gagtgcgggg aacgaatctt caccagaacg
     8701 gtgagacagg ttaagcagca gacaacggtt cattatttcg tatcacctcc acggccgcct
     8761 gttaagacga acccacaagc caaaactctg atttcaaccc ggctggaagt ggcaacacga
     8821 aagaaacgtc gtgtgctttt tatttaagcc gcgcgccgcg ttttataccc cacaatggca
     8881 gaaaattgca aaagataaat acgcagaatg ccggcattgt caggaaaaat ttccagccac
     8941 gtttttaaca gaatgagaca cgattcaaaa aaaagtggaa atcgggtgaa gaattgacct
     9001 aaaatagcca tccagatgtt aatccatcca taccgattaa cactcagact gccagtgttt
     9061 ttaacctgca gagtcgtggt aggatccgct accacagaaa atccacacaa cagtttgagc
     9121 taaccaaatt ctctttaggt gatattaaat atggcaaaac acctttttac gtccgagtcc
     9181 gtctctgaag ggcatcctga caaaattgct gaccaaatct ctgatgccgt tttagacgcg
     9241 atcctcgaac aggatccgaa agcacgcgtt gcttgcgaaa cctacgtaaa aaccggcatg
     9301 gttttagttg gcggcgaaat caccaccagc gcctgggtag acatcgaaga gatcacccgt
     9361 aacaccgttc gcgaaattgg ctatgtacat tccgacatgg gctttgacgc taactcctgt
     9421 gcggttctga gcgctatcgg caaacagtct cctgacatca accagggcgt tgaccgtgcc
     9481 gatccgctgg aacagggcgc gggtgaccag ggtctgatgt ttggctacgc aactaatgaa
     9541 accgacgtgc tgatgccagc acctatcacc tatgcacacc gtctggtaca gcgtcaggct
     9601 gaagtgcgta aaaacggcac tctgccgtgg ctgcgcccgg acgcgaaaag ccaggtgact
     9661 ttccagtatg acgacggcaa aatcgttggt atcgatgctg tcgtgctttc cactcagcac
     9721 tctgaagaga tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat catcaagccg
     9781 attctgcccg ctgaatggct gacttctgcc accaaattct tcatcaaccc gaccggtcgt
     9841 tttgttatcg gtggcccgat gggtgactgc ggtctgactg gtcgtaaaat tatcgttgat
     9901 acctacggcg gcatggcacg tcacggtggc ggagcattct ctggtaaaga tccatcaaaa
     9961 gtggaccgtt ccgctgcgta cgcagcacgt tatgttgcaa aaaacatcgt tgctgctggc
    10021 ctagccgatc gttgtgaaat tcaggtttcc tacgcaatcg gcgtggctga accgacttcc
    10081 atcatggtag aaactttcgg taccgagaaa gtgccttctg aacaactgac tctgctggta
    10141 cgtgagttct tcgacctgcg cccatacggt ctgattcaga tgctggatct gctgcacccg
    10201 atctacaaag aaaccgcagc atacggtcac tttggtcgtg aacatttccc gtgggaaaaa
    10261 accgacaaag cgcagctgct gcgcgatgct gccggtctga agtaatcttt cttcacctgc
    10321 tttcaaaggc cagcctcgcg ctggcctttt tcttttacct caccgcggtg gataggcgtt
    10381 cacgccgcat ccggcaaaaa aaccgcctgc acaataacat cattcttcct gatcacgttt
    10441 caccgcaaat tatcatcaca actgaaaccg attacaccaa ccacaacaga caaagatttg
    10501 taatattttc atattattat tcggttttca cagttgttac atttcttttc agtaaagtct
//
LOCUS       DAZKFU020000166        10521 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZKFU020000166 DAZKFU020000000
VERSION     DAZKFU020000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.167"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            237..641
                     /gene="sdhC"
                     /locus_tag="ACHOQQ_RS16985"
                     /old_locus_tag="pgaptmp_003397"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             237..641
                     /gene="sdhC"
                     /locus_tag="ACHOQQ_RS16985"
                     /old_locus_tag="pgaptmp_003397"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4471609.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            635..982
                     /gene="sdhD"
                     /locus_tag="ACHOQQ_RS16990"
                     /old_locus_tag="pgaptmp_003398"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             635..982
                     /gene="sdhD"
                     /locus_tag="ACHOQQ_RS16990"
                     /old_locus_tag="pgaptmp_003398"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGU4471610.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            982..2748
                     /gene="sdhA"
                     /locus_tag="ACHOQQ_RS16995"
                     /old_locus_tag="pgaptmp_003399"
     CDS             982..2748
                     /gene="sdhA"
                     /locus_tag="ACHOQQ_RS16995"
                     /old_locus_tag="pgaptmp_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGU4471611.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2764..3480
                     /gene="sdhB"
                     /locus_tag="ACHOQQ_RS17000"
                     /old_locus_tag="pgaptmp_003400"
     CDS             2764..3480
                     /gene="sdhB"
                     /locus_tag="ACHOQQ_RS17000"
                     /old_locus_tag="pgaptmp_003400"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGU4471612.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3781..6582
                     /gene="sucA"
                     /locus_tag="ACHOQQ_RS17005"
                     /old_locus_tag="pgaptmp_003401"
     CDS             3781..6582
                     /gene="sucA"
                     /locus_tag="ACHOQQ_RS17005"
                     /old_locus_tag="pgaptmp_003401"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGU4471613.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6597..7814
                     /gene="odhB"
                     /locus_tag="ACHOQQ_RS17010"
                     /old_locus_tag="pgaptmp_003402"
     CDS             6597..7814
                     /gene="odhB"
                     /locus_tag="ACHOQQ_RS17010"
                     /old_locus_tag="pgaptmp_003402"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGU4471614.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            8090..9256
                     /gene="sucC"
                     /locus_tag="ACHOQQ_RS17015"
                     /old_locus_tag="pgaptmp_003403"
     CDS             8090..9256
                     /gene="sucC"
                     /locus_tag="ACHOQQ_RS17015"
                     /old_locus_tag="pgaptmp_003403"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGU4471615.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9256..10125
                     /gene="sucD"
                     /locus_tag="ACHOQQ_RS17020"
                     /old_locus_tag="pgaptmp_003404"
     CDS             9256..10125
                     /gene="sucD"
                     /locus_tag="ACHOQQ_RS17020"
                     /old_locus_tag="pgaptmp_003404"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGU4471616.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         2486 a         2742 c         2940 g         2353 t
ORIGIN      
        1 tgattttgtg aacggcctat actgccgcca ggtctccgga acaccctgca atcccgagcc
       61 acccagcgtt gtaacgtgtc gttttcgcat ctggaagcag tgttttgcat gacgcgcagt
      121 tatagaaagg acgctgtctg acccgcaagc agaccggagg aaggaaatcc cgacgtctcc
      181 aggtaacaga aagttaacct ctgtgcccgt agtccccagg gaataataag aacagcatgt
      241 gggcgttatt catgataaga aatgtgaaaa aacaaagacc tgttaatctg gacctacaga
      301 ccatccggtt ccccatcacg gcgatagcgt ccattctcca tcgcgtttcc ggtgtgatca
      361 cctttgttgc agtgggcatc ctgctgtggc ttctgggtac cagcctctct tcccctgaag
      421 gtttcgagca agcttccgcg attatgggca gcttcttcgt caaatttatc atgtggggca
      481 tccttaccgc tctggcgtat cacgtcgtcg taggtattcg ccacatgatg atggattttg
      541 gctatctgga agaaacattc gaagcgggta aacgctccgc caaaatctcc tttgttatta
      601 ctgtcgtgct ttcacttctc gcaggagtcc tcgtatggta agcaacgcct ccgcattagg
      661 acgcaatggc gtacatgatt tcatcctcgt tcgcgctacc gctatcgtcc tgacgctcta
      721 catcatttat atggtcggtt ttttcgctac cagtggcgag ctgacatatg aagtctggat
      781 cggtttcttc gcctctgcgt tcaccaaagt gttcaccctg ctggcgctgt tttctatctt
      841 gatccatgcc tggatcggca tgtggcaggt gttgaccgac tacgttaaac cgctggcttt
      901 gcgcctgatg ctgcaactgg tgattgtcgt tgcactggtg gtttacgtga tttatggatt
      961 cgttgtggtg tggggtgtgt gatgaaattg ccagtcagag aatttgatgc agttgtgatt
     1021 ggtgccggcg gcgcaggtat gcgcgcggcg ctgcaaattt cccagagcgg ccagacctgt
     1081 gcgctgctct ctaaagtctt cccgacccgt tcccataccg tttctgcgca aggcggcatt
     1141 accgttgcgc tgggtaatac ccatgaagat aactgggaat ggcatatgta cgacaccgtg
     1201 aaagggtcgg actatatcgg tgaccaggac gcgattgaat atatgtgtaa aaccgggccg
     1261 gaagcgattc tggaactcga acacatgggc ctgccgttct cgcgtctcga tgatggtcgt
     1321 atctatcaac gtccgtttgg cggtcagtcg aaaaacttcg gcggcgagca ggcggcacgc
     1381 actgcggcag cggctgaccg taccggtcac gcactgttgc acacgcttta tcagcagaac
     1441 ctgaaaaacc acaccaccat tttctccgag tggtatgcgc tggatctggt gaaaaaccag
     1501 gatggcgcgg tggtgggttg taccgcactg tgcatcgaaa ccggtgaagt ggtttatttt
     1561 aaagcccgcg ctaccgtgct ggcgactggc ggagcagggc gtatttatca gtccaccact
     1621 aacgcccaca ttaacactgg cgacggtgtc ggcatggcta tccgcgcagg cgtaccggtg
     1681 caggatatgg aaatgtggca attccacccg accggcattg ccggtgcggg cgtactggtc
     1741 accgaaggtt gccgtggtga aggcggttat ctgctgaaca aacatggcga acgctttatg
     1801 gagcgttatg cgccgaacgc caaagacctg gcgggccgtg atgtggtggc gcgttccatc
     1861 atgattgaaa tccgtgaagg tcgcggctgt gatggtccgt gggggccaca cgcgaaactg
     1921 aaactcgatc acttgggtaa agaagttctc gaatcccgtc tgccgggtat cctggagctt
     1981 tcccgtacct tcgctcacgt tgatccggtg aaagagccga ttccggttat cccaacctgt
     2041 cactacatga tgggcggtat tccgaccaaa gttaccggtc aggcgctgac tgtgaatgag
     2101 aaaggcgaag atgtggttgt tccggggctg ttcgcggtag gtgaaattgc ctgcgtatcg
     2161 gtacacggtg ctaaccgtct gggcggcaac tcgctgctgg acctggtggt ctttggtcgc
     2221 gcagcgggtc tgcatctgca agagtctatc gccgagcagg gcgcattgcg cgatgccagc
     2281 gagtctgatg ttgaagcatc tctggatcgt ctgaaccgct ggaacaataa tcgtaacggt
     2341 gaagatccgg tggcgattcg taaagcgctg caagaatgta tgcagcataa cttctcggtc
     2401 ttccgtgaag gtgatgcgat ggcgaaaggg cttgagcagt tgaaagttat ccgcgagcgt
     2461 ctgaaaaatg cccgtctgga tgacacttcc agcgagttca acacccagcg cgttgagtgt
     2521 ctggaactgg ataacctgat ggaaacggcg tatgcaacgg ctgtttctgc caacttccgt
     2581 accgaaagcc gtggcgcgca tagccgcttc gacttcccgg atcgtgatga tgaaaactgg
     2641 ctgtgccact ccctgtatct gccagagtcg gaatccatga cgcgccgaag cgtcaacatg
     2701 gaaccgaaac tgcgcccggc attcccgccg aagattcgta cttactaatg cggagacagg
     2761 aaaatgagac tcgagttttc aatttatcgc tataacccgg atgttgatga tgctccgcgt
     2821 atgcaggatt acaccctgga agcggaagaa ggtcgcgaca tgatgctgct ggatgcgctt
     2881 atccagctga aagagaaaga tcccagcctg tcgttccgcc gctcctgccg tgaaggtgtg
     2941 tgcggttccg acggtctgaa catgaacggc aagaatggtc tggcctgtat taccccgatt
     3001 tcggcactca accagccggg caagaagatt gtgattcgcc cgctgccagg tttaccggtg
     3061 atccgcgatt tggtggtaga catgggacaa ttctatgcgc aatatgagaa aattaagcct
     3121 tacctgttga ataatggaca aaatccgcca gctcgcgagc atttacagat gccagagcag
     3181 cgcgaaaaac tcgacgggct gtatgaatgt attctctgcg catgttgttc aacctcttgt
     3241 ccgtctttct ggtggaatcc cgataagttt atcggcccgg caggcttgtt agcggcatat
     3301 cgtttcctga tcgatagccg tgataccgag actgacagcc gcctcgacgg tttgagcgat
     3361 gcattcagtg tattccgctg tcacagcatc atgaactgcg tcagtgtatg tccgaaggga
     3421 ctgaacccga cgcgcgccat cggtcatatc aagtcgatgt tgttgcaacg taatgcgtaa
     3481 accgtaggcc tgataagacg cgcaagcgtc gcatcaggca accagtgccg gatgcggcgt
     3541 gaacgcctta tccggcctac aagtcattac ccgtaggcct gataagcgca gcgcatcagg
     3601 cgtaacaaag aaatgcagga aatctttaaa aactgcccct gacactaaga cagtttttaa
     3661 aggttccttc gcgagccact acgtagacaa gagctcgcaa gtgaaccccg gcacgcacat
     3721 cactgtgcgt ggtagtatcc acggcgaagt aagcataaaa aagatgctta agggatcacg
     3781 atgcagaaca gcgctttgaa agcctggttg gactcttctt acctctctgg cgcaaaccag
     3841 agctggatag aacagctcta tgaagacttc ttaaccgatc ctgactcggt tgacgctaac
     3901 tggcgttcga cgttccagca gttacctggt acgggagtca aaccggatca attccactct
     3961 caaacgcgtg aatatttccg ccgcctggcg aaagacgctt cacgttactc ttcaacgatc
     4021 tccgaccctg acaccaatgt gaagcaggtt aaagtcctgc agctcattaa cgcataccgc
     4081 ttccgtggtc accagcatgc gaatctcgat ccgctgggac tgtggcagca agataaagtg
     4141 gccgatctgg atccgtcttt ccacgatctg accgaagcag acttccagga gaccttcaac
     4201 gtcggttcat ttgccagcgg caaagaaacc atgaaactcg gcgagctgct ggaagccctc
     4261 aagcaaacct actgtggccc gattggtgcc gagtatatgc acatcaccag caccgaagaa
     4321 aaacgctgga tccaacagcg tatcgagtct ggtcgcgcga ctttcaatag cgaagagaaa
     4381 aaacgcttct taagcgaact gaccgccgct gaaggccttg aacgttacct cggtgcaaaa
     4441 ttccctggtg caaaacgctt ctcgctggaa ggcggtgacg cgttaatccc gatgcttaaa
     4501 gagatgatcc gccacgctgg caacagcggc acccgggaag tggttctcgg gatggcgcac
     4561 cgtggtcgtc tgaacgtgct ggtgaacgtg ctgggtaaaa aaccgcaaga cttgttcgac
     4621 gagtttgccg gtaaacataa agaacacctc ggcacgggtg acgtgaaata ccacatgggc
     4681 ttctcgtctg acttccagac cgatggcggc ctggtgcacc tggcgctggc gtttaacccg
     4741 tctcaccttg agattgtaag cccggtagtt atcggctctg ttcgtgcccg tctggacaga
     4801 cttgatgagc cgagcagcaa caaagtgctg ccaatcacca tccacggtga cgccgcagtg
     4861 accgggcagg gcgtggttca ggaaactctg aacatgtcga aagcgcgtgg ttatgaagtt
     4921 ggcggtacgg tacgtatcgt tatcaacaac caggttggtt tcaccacctc taatccgctg
     4981 gatgcccgtt ctacaccgta ctgtactgat atcggtaaga tggttcaggc accgattttc
     5041 cacgttaacg cggatgatcc ggaagccgtt gcctttgtga cccgtctggc gctcgatttc
     5101 cgtaacacct ttaaacgtga tgtcttcatc gacctggtgt gctaccgccg tcacggccac
     5161 aacgaagccg acgagccgag cgcaacccag ccgctgatgt atcagaaaat caaaaaacat
     5221 ccgacaccgc gcaaaatcta cgctgacaag ctggagcagg aaaaagtggc gacgctggaa
     5281 gatgccaccg agatggttaa cctgtaccgc gatgcgctgg atgctggcga ttgcgtagtg
     5341 gcagagtggc gtccgatgaa catgcactct ttcacctggt cgccgtatct caaccacgaa
     5401 tgggacgaag agtacccgaa caaagttgag atgaagcgcc tgcaggagct ggcgaaacgc
     5461 atcagcacgg tgccggaagc ggttgaaatg cagtctcgcg ttgccaaaat ttatggcgat
     5521 cgccaggcga tggctgccgg tgagaaactg ttcgactggg gcggcgcgga aaacctcgct
     5581 tacgccacgc tggttgacga aggcattccg gttcgcctgt cgggtgaaga ctccggtcgc
     5641 ggtacattct tccaccgcca cgcggtgatc cacaaccagt ctaacggttc cacttacacg
     5701 ccgctgcaac acatccataa cggccagggc gcgttccgtg tctgggactc cgtactttct
     5761 gaagaagcag tgctggcgtt tgaatacggt tatgccaccg cagaaccacg cactctgact
     5821 atctgggaag cgcagttcgg tgacttcgcc aacggtgcgc aggtggttat cgaccagttc
     5881 atctcctctg gcgaacagaa atggggccgg atgtgtgggc tggtgatgtt gctgccgcac
     5941 ggttacgaag ggcaggggcc ggagcactcc tccgcgcgtc tggaacgtta tctgcaactt
     6001 tgtgctgagc aaaacatgca ggtgtgcgta ccgtctaccc cggcacaggt ttaccacatg
     6061 ctgcgtcgtc aggcgctgcg cgggatgcgt cgtccgctgg tcgtgatgtc gccgaaatcc
     6121 ctgctgcgtc atccgctggc ggtttccagc ctcgaagaac tggcgaacgg caccttcctg
     6181 ccagccatcg gtgaaatcga cgagcttgat ccgaagggcg tgaagcgcgt agtgatgtgt
     6241 tctggtaagg tttattacga cctgctggaa caacgtcgta agaacaatca acacgatgtc
     6301 gccattgtgc gtatcgagca actctacccg ttcccgcata aagcgatgca ggaagtgttg
     6361 cagcagtttg ctcacgtcaa ggattttgtc tggtgccaag aagagccgct caaccagggc
     6421 gcatggtact gcagccagca tcatttccgt gaagtgattc cgtttggggc ttctctgcgt
     6481 tatgcaggcc gcccggcctc cgcctctccg gcggtagggt atatgtccgt tcaccagaaa
     6541 cagcaacaag atctggttaa tgacgcgctg aacgtcgaat aaataaagga tacacaatga
     6601 gtagcgtaga tattctggtc cctgacctgc ctgaatccgt agccgatgcc accgtcgcaa
     6661 cctggcataa aaaacccggc gacgcagtcg tacgtgatga agtgctggta gaaatcgaaa
     6721 ctgacaaagt ggtactggaa gtaccggcat cagcagacgg cattctggat gcggttctgg
     6781 aagatgaagg tacaacggta acgtctcgtc agatccttgg tcgcctgcgt gaaggcaaca
     6841 gcgccggtaa agaaaccagc gccaaatctg aagagaaagc gtccactccg gcgcaacgcc
     6901 agcaggcgtc tctggaagag caaaacaacg atgcgttaag cccagcgatc cgtcgcctgc
     6961 tggctgaaca caacctcgac gccagcgcca ttaaaggcac cggtgtgggt ggtcgtctga
     7021 ctcgtgaaga tgtggaaaaa catctggcga aagccccggc gaaagagtct gctccggcag
     7081 cggctgctcc ggcggcgcaa ccggctctgg ctgcacgtag cgaaaaacgt gtcccgatga
     7141 ctcgcctgcg taagcgtgtg gcagagcgtc tgctggaagc gaaaaactcc accgccatgc
     7201 tgaccacgtt caacgaagtc aacatgaagc cgattatgga tctgcgtaag cagtacggtg
     7261 aagcgtttga aaaacgccac ggcatccgtc tgggctttat gtccttctac gtgaaagcgg
     7321 tggttgaagc cctgaaacgt tacccggaag tgaatgcttc tatcgacggc gatgacgtgg
     7381 tttaccacaa ctatttcgac gtcagcatgg cggtttctac gccgcgcggc ctggtgacgc
     7441 cggttctgcg tgatgtcgat accctcggca tggcagacat cgagaagaaa atcaaagagc
     7501 tggcagttaa aggccgtgac ggcaagctga cggttgaaga tctgaccggt ggtaacttca
     7561 ccatcaccaa cggtggtgtg ttcggttccc tgatgtctac gccgatcatc aacccgccgc
     7621 agagcgcaat tctgggtatg cacgctatca aagatcgtcc gatggcggtg aatggtcagg
     7681 ttgagatcct gccgatgatg tacctggcgc tgtcctacga tcaccgtctg atcgatggtc
     7741 gcgaatccgt gggcttcctg gtaacgatca aagagttgct ggaagatccg acgcgtctgc
     7801 tactggacgt gtagtagttt aagtttcacc tgcactgtag accggataag acattatcgc
     7861 cttctccggc aattgaagcc tgatgcgacg cttaacgcgt cttatcaggc ctacgggaag
     7921 caccgatgta ggtcggataa ggcgctcgcg ccgcatccga caagcgatgc cagatgcgac
     7981 gcttacacgt cttatcaggc ctacgggtga ccgacaatac ccgggagcga tacgaaatat
     8041 tcggtctacg gtttaaaaga taacgattac tgaaggatgg acagaacaca tgaacttaca
     8101 tgaatatcag gcaaaacaac tgtttgcccg ctatggctta ccagcaccgg tgggttatgc
     8161 ctgtactact ccgcgcgaag cagaagaagc cgcttcaaaa atcggtgccg gtccgtgggt
     8221 agtgaaatgt caggttcacg ctggtggccg cggtaaagcg ggcggtgtga aagttgtaaa
     8281 cagcaaagaa gacatccgtg cttttgcaga aaactggctc ggcaagcgtc tggtaacgta
     8341 tcaaacagat gctaatggcc aaccggttaa ccagattctg gttgaagcag cgaccgatat
     8401 cgctaaagag ctgtatctcg gtgccgttgt agaccgtagt tcccgtcgcg tggtctttat
     8461 ggcctctacc gaaggcggcg tggaaatcga aaaagtggcg gaagaaactc cgcacctgat
     8521 ccacaaagtt gcgcttgatc cgctgactgg cccgatgccg tatcagggac gcgagctggc
     8581 gttcaaactg ggtctggaag gtaaactggt tcagcagttc accaaaatct tcatgggcct
     8641 ggcgaccatt ttcctggagc gcgacctggc gttgatcgaa atcaacccgc tggtcatcac
     8701 caaacagggt gatctgattt gcctcgacgg caaactgggc gctgatggca acgccctgtt
     8761 ccgccagcct gatctgcgcg aaatgcgtga ccagtcgcag gaagatccac gtgaagcaca
     8821 ggctgcacag tgggaactga actacgttgc gctggacggt aacatcggtt gtatggttaa
     8881 cggcgcaggt ctggcgatgg gtacgatgga catcgttaaa ctgcacggcg gagaaccggc
     8941 taacttcctg gacgttggcg gcggcgcaac caaagaacgt gtaaccgaag cgttcaaaat
     9001 catcctctct gacgacaaag tgaaagccgt tctggttaac atcttcggcg gtatcgttcg
     9061 ttgcgatctg atcgctgacg gtatcatcgg cgcggtagcg gaagtgggtg ttaacgtacc
     9121 ggtcgtggta cgtctggaag gtaacaacgc cgaactcggc gcgaagaaac tggctgacag
     9181 cggcctgaat attattgcag caaaaggtct gacggatgca gctcagcagg ttgttgccgc
     9241 agtggagggg aaataatgtc cattttaatt gataaaaaca ccaaggttat ctgccagggc
     9301 tttaccggta gccaggggac tttccactct gaacaggcca ttgcatacgg caccaaaatg
     9361 gttggtggcg taactccagg taaaggcggt actacccacc tcggcctgcc ggtgttcaac
     9421 accgtgcgtg aagctgttgc tgctactggc gctaccgctt ctgttatcta cgtaccagca
     9481 ccgttctgca aagactccat tctggaagcc atcgacgcag gcatcaaact gattatcacc
     9541 atcactgaag gcatcccgac gctggatatg ctgactgtga aagtgaaact ggatgaagcc
     9601 ggcgttcgta tgatcggccc gaactgccca ggcgttatca ccccgggtga atgcaaaatt
     9661 ggtatccaac cgggtcacat tcacaaaccg ggtaaagtgg gtatcgtttc ccgttccggt
     9721 acactgacct atgaagcggt taaacagacc acggattacg gtttcggtca gtcgacctgt
     9781 gtcggtatcg gcggtgaccc gatccctggc tctaacttta tcgacattct cgaaatgttc
     9841 gaaaaagatc cgcagaccga agcgatcgtg atgatcggtg agatcggcgg tagcgctgaa
     9901 gaagaagctg ctgcgtacat caaagagcac gttaccaagc cagttgtggg ttacatcgct
     9961 ggtgtgactg cgccgaaagg caaacgtatg ggccacgcgg gtgccatcat tgccggtggg
    10021 aaagggactg cggatgagaa attcgctgct ctggaagccg caggcgtgaa aaccgttcgc
    10081 agcctggcgg atatcggtga agcactgaaa actgttctga aataaatact tgtaataaga
    10141 aatagccctc gccgcttccc tctacaggaa tggcgaaggg ctgtcggttt cgacatggtt
    10201 ggccatcgta tgatggcctt ttttgtgcct taaacgttaa ccacggtcta aatctgactg
    10261 caggcttgac tgcatgacca cagtcactgt ggattgccgc aaccgaccgc aagccacgaa
    10321 taagaacgac ggcgaatttc gaatcgttac ctgatgcact ggcttcccag cgtttgaccg
    10381 tagcgttaag taaatcaaca aaagcgatgt tcaccgcttt tcgtcaagtc gtgccatttg
    10441 tcagccagga tcgggtcttt accccggata ttgcagcatc gcaaaaattc ttagagggat
    10501 atgctataaa tcaaaaataa a
//
LOCUS       DAZKFU020000167        10455 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZKFU020000167 DAZKFU020000000
VERSION     DAZKFU020000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.168"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..417
                     /locus_tag="ACHOQQ_RS17025"
                     /old_locus_tag="pgaptmp_003405"
     CDS             88..417
                     /locus_tag="ACHOQQ_RS17025"
                     /old_locus_tag="pgaptmp_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4471617.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLIERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGCDGIARVFYCTLAGKRIIMLHSFIKKTQKTPPAELKKAETRM
                     KEVKHDW"
     gene            407..715
                     /locus_tag="ACHOQQ_RS17030"
                     /old_locus_tag="pgaptmp_003406"
     CDS             407..715
                     /locus_tag="ACHOQQ_RS17030"
                     /old_locus_tag="pgaptmp_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4471618.1"
                     /translation="MTGKRNPPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            734..1039
                     /locus_tag="ACHOQQ_RS17035"
                     /old_locus_tag="pgaptmp_003407"
     CDS             734..1039
                     /locus_tag="ACHOQQ_RS17035"
                     /old_locus_tag="pgaptmp_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4471619.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NATISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(1082..1819)
                     /locus_tag="ACHOQQ_RS17040"
                     /old_locus_tag="pgaptmp_003408"
     CDS             complement(1082..1819)
                     /locus_tag="ACHOQQ_RS17040"
                     /old_locus_tag="pgaptmp_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471620.1"
                     /translation="MKNNVMKNGTIDSQALLKMVNEARRLCGEKEVRNNDFIARIKDE
                     LDGEGYEIFVTPMDKKKGGADQVVIVMTYKQALRVAARESKSVRRSLVDQLESMQQQL
                     QQKITSKHSTNGLEEFRKARALKMTVDTMKDLFGFLPNLAPEAKQVVAASLVNPVVGA
                     NVIPLPMINEHYYSASEVGSQLKISANKVGRIANTYMLKTEQYGKWFIDKSPYSDKQV
                     ESFRYNNRGVRKIEEILEAENKAEFGT"
     gene            complement(1915..2475)
                     /locus_tag="ACHOQQ_RS17045"
                     /old_locus_tag="pgaptmp_003409"
     CDS             complement(1915..2475)
                     /locus_tag="ACHOQQ_RS17045"
                     /old_locus_tag="pgaptmp_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4471621.1"
                     /translation="MIEGISNKSLNTACVDGGLLYKVTFTQIDNKDNAFTVIYTSPEM
                     AKKWVDLHRLCDFRVEWGCYEMRCRWHHRGDEKMSTTLEQWLEQQHGKIDVDCGCVST
                     ETLMHWMRVAYEAGNSPVTPDGWISCSERMPDDGQWCVVNTEYGYYVQCWSEGQGWLG
                     DYISIPECDVINWMPLPEPPQEVNRG"
     gene            complement(2472..2837)
                     /locus_tag="ACHOQQ_RS17050"
                     /old_locus_tag="pgaptmp_003410"
     CDS             complement(2472..2837)
                     /locus_tag="ACHOQQ_RS17050"
                     /old_locus_tag="pgaptmp_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4471622.1"
                     /translation="MSEINYQALRDAAVAIETVATPQKLLAFRMKVTPSVVLALLDEI
                     KRLEDTNIDAMCRIAELEAREAELPDGYEPRMGHPINSGERVVMMPHHFGGWLDRFDV
                     EHALQAAGIKFKTAGGEYE"
     gene            complement(2834..3073)
                     /locus_tag="ACHOQQ_RS17055"
                     /old_locus_tag="pgaptmp_003411"
     CDS             complement(2834..3073)
                     /locus_tag="ACHOQQ_RS17055"
                     /old_locus_tag="pgaptmp_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471623.1"
                     /translation="MFKQNEKSIAQIAEYIPRTCRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNCHTRESFFSCIAAQLHKGGNQ"
     gene            complement(3066..3269)
                     /locus_tag="ACHOQQ_RS17060"
                     /old_locus_tag="pgaptmp_003412"
     CDS             complement(3066..3269)
                     /locus_tag="ACHOQQ_RS17060"
                     /old_locus_tag="pgaptmp_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471624.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            complement(3266..4144)
                     /locus_tag="ACHOQQ_RS17065"
                     /old_locus_tag="pgaptmp_003413"
     CDS             complement(3266..4144)
                     /locus_tag="ACHOQQ_RS17065"
                     /old_locus_tag="pgaptmp_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HGU4471625.1"
                     /translation="MSTHHVVSFSGGRTSAYLVHLMEQRRKAGENVHYILMDTGCEHP
                     LTYRFIREVVKFWDIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGQGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWRKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYSNLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(4252..4695)
                     /locus_tag="ACHOQQ_RS17070"
                     /old_locus_tag="pgaptmp_003414"
     CDS             complement(4252..4695)
                     /locus_tag="ACHOQQ_RS17070"
                     /old_locus_tag="pgaptmp_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471626.1"
                     /translation="MQFKDLPEDIQKIAADTLKAHLSVLDLTKEPKANLENISRNVRN
                     VFVGLYAYDNEKHEEHIQNGCINKCQQNIKLPVADKTEQKTDVLVFWNTVEAIARSTM
                     LEFNNINHSGIDTHQSSHREGFSILLTLLQEQGEYPQPTSNDTEQ"
     gene            complement(4772..5593)
                     /locus_tag="ACHOQQ_RS17075"
                     /old_locus_tag="pgaptmp_003415"
     CDS             complement(4772..5593)
                     /locus_tag="ACHOQQ_RS17075"
                     /old_locus_tag="pgaptmp_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HGU4471627.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADEHSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKRQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAIRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGNRE
                     FTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEEEDILTFIGTFSV"
     gene            complement(5657..6004)
                     /locus_tag="ACHOQQ_RS17080"
                     /old_locus_tag="pgaptmp_003416"
     CDS             complement(5657..6004)
                     /locus_tag="ACHOQQ_RS17080"
                     /old_locus_tag="pgaptmp_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471628.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            complement(6079..6666)
                     /locus_tag="ACHOQQ_RS17085"
                     /old_locus_tag="pgaptmp_003417"
     CDS             complement(6079..6666)
                     /locus_tag="ACHOQQ_RS17085"
                     /old_locus_tag="pgaptmp_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HGU4471629.1"
                     /translation="MDEVIFTYNEESALTAGQGGFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADGYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANMVSTLKDKDNRRKNVNPNHTDDPGYWQYGSDSF"
     gene            complement(6666..7355)
                     /locus_tag="ACHOQQ_RS17090"
                     /old_locus_tag="pgaptmp_003418"
     CDS             complement(6666..7355)
                     /locus_tag="ACHOQQ_RS17090"
                     /old_locus_tag="pgaptmp_003418"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU4471630.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVSE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFDAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGVNN"
     gene            complement(7352..8302)
                     /locus_tag="ACHOQQ_RS17095"
                     /old_locus_tag="pgaptmp_003419"
     CDS             complement(7352..8302)
                     /locus_tag="ACHOQQ_RS17095"
                     /old_locus_tag="pgaptmp_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HGU4471631.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARANLPSAQNAIINVAAIGITLNPASKLAYLLPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLSTQA
                     GEGIILNADHIPERDVTPASDEIINEITQAITEINKTWDDLLPLCSKTFRRTIASHEY
                     LSQEEAVKTLDFVKKKAARNKATAEAKIHATTENNSEAVS"
     gene            complement(8320..8601)
                     /gene="gamL"
                     /locus_tag="ACHOQQ_RS17100"
                     /old_locus_tag="pgaptmp_003420"
     CDS             complement(8320..8601)
                     /gene="gamL"
                     /locus_tag="ACHOQQ_RS17100"
                     /old_locus_tag="pgaptmp_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HGU4471632.1"
                     /translation="MNAYLTCDRIEERRWVNQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            complement(8622..8903)
                     /locus_tag="ACHOQQ_RS17105"
                     /old_locus_tag="pgaptmp_003421"
     CDS             complement(8622..8903)
                     /locus_tag="ACHOQQ_RS17105"
                     /old_locus_tag="pgaptmp_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU4471633.1"
                     /translation="MLNQEININVKSVITPAKTIMGEVFMDDKIIAYFVVLPDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            complement(8915..9127)
                     /locus_tag="ACHOQQ_RS17110"
                     /old_locus_tag="pgaptmp_003422"
     CDS             complement(8915..9127)
                     /locus_tag="ACHOQQ_RS17110"
                     /old_locus_tag="pgaptmp_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HGU4471634.1"
                     /translation="MLKPHYGTALVSREEIKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            complement(9198..9971)
                     /locus_tag="ACHOQQ_RS17115"
                     /old_locus_tag="pgaptmp_003423"
                     /pseudo
     CDS             complement(9198..9971)
                     /locus_tag="ACHOQQ_RS17115"
                     /old_locus_tag="pgaptmp_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529252.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT         2497 a         2539 c         2410 g         3009 t
ORIGIN      
        1 gatctaacag catgactgtc tgtttgatat gtgttgtcgt caacacagac cttgaatgca
       61 tataacaaat ttgttatgtt cattctcatg aactacacta tcgaatacta cagtgaagag
      121 gtccggctgg aagtcgatca gcttccattg agtatgcgtg cccgatacca acatctcatt
      181 gaacgtatga aggtatacgg cagcaatctc ggagaacctc acaccagcgc cttcggtgac
      241 ggacttttcg agcttcggat taaaggctgt gatggcatcg cacgcgtttt ttactgcact
      301 ttggcaggaa aacgcatcat catgctgcac agtttcatca agaagactca gaaaacccca
      361 ccggctgaac tcaaaaaagc tgaaaccaga atgaaggagg ttaagcatga ctggtaaacg
      421 taacccacca accatgacgc acgatgaaat ggcagctaaa tggatggagg atcctgcttt
      481 taaagcagaa tacgacgcca tcgccgatga attcgcactg cttgatgaaa tgctggcagc
      541 acgaaaagaa gctggcttaa cccaggctga agttgctgaa cgtatgggaa caaaagcgac
      601 tgcgatcacc agaatggaaa gcaatcttgc atcaggtata agtggcccat catttgccac
      661 actgaaaaaa ttcgcccgtg ctacagggaa aaaactccag atccgcttcg tttaactacg
      721 aggattacca ctaatggcga tgtttaaccc ccctcatccc ggagaaatta ttgctgatat
      781 tctggaagat cagaatattg gaatcaggga actggcaaga gcgcttgatg tcgcgccttc
      841 cactgttcag cgactggttt caggtaatgc gacaatatct cctgaaatgg ctgttcgtct
      901 cgctgctgtc ctgggtggaa ctccgtcttc atggattcgt ctccagacgg catggagcct
      961 cgaaaaagcg gaaagagaag ttgacgtatc tcatctctca acaaaatacc gcccggcaga
     1021 aatttcgcct catgcttaac caccgcgccg tcattctggc ggcgtcggag agtggaggat
     1081 atcaggttcc gaactctgct ttgttctccg cctccaggat ttcttcaatc ttccgtactc
     1141 cccgattatt gtatcgaaag ctttctacct gcttatccga atacggggat ttgtcgatga
     1201 accatttccc gtattgttct gttttgagca tgtaggtatt ggcaatgcga ccaaccttgt
     1261 tcgcggatat tttgagttgt gatccaactt ctgacgccga atagtaatgt tcattgatca
     1321 tcggtagcgg gattacgtta gcgcctacaa cgggattaac caggctggcg gcaacgacct
     1381 gtttagcttc aggggcaagg ttggggagga agccgaaaag gtctttcatt gtatcgacgg
     1441 tcattttcag cgcccgtgct ttacggaatt cctcaaggcc gttagtcgaa tgtttcgagg
     1501 tgattttttg ttgcagttgc tgttgcattg actccagttg atcgaccagc gaacgacgga
     1561 cggatttaga ttcacgagcg gcaacccgaa gcgcttgttt ataggtcatc acaataacaa
     1621 cctgatccgc accgcctttt ttcttatcca tgggggttac gaaaatttcg taaccctccc
     1681 catcaagttc atctttgatg cgtgcaatga agtcgttgtt gcgtacttct ttttcaccgc
     1741 acagtctccg cgcttcattc accattttca acagagcctg gctgtcgata gttccatttt
     1801 tcattacatt atttttcatc gttttaacct cttaagctcg ccgtagcgag ttcagataaa
     1861 agaaatcccc gcgagtgcga ggattcttat tcacctttga cggcaagttg caggttagcc
     1921 acggttaacc tcctgcggcg gttctggtag cggcatccaa ttgattacat cgcattcagg
     1981 gatgctgata taatcaccaa gccatccttg accttcagac cagcattgca cgtaataccc
     2041 gtattctgtg ttcactacgc accactgccc gtcatccggc attcgctcac tacagcttat
     2101 ccaaccatcc ggagttaccg gagagttgcc agcctcatac gccacacgca tccagtgcat
     2161 aagcgtttca gtgctcacac agccgcaatc aacatcaatt ttgccgtgct gctgttccag
     2221 ccattgctca agcgtagtgc tcattttttc atccccccga tgatgccatc tgcatcgcat
     2281 ttcatagcac ccccactcaa cacgaaaatc acatagcctg tgcaggtcca cccacttctt
     2341 cgccatctct ggcgacgtgt aaatcacagt gaatgcgtta tctttgttgt cgatttgcgt
     2401 aaacgtcact ttgtacaaca acccaccgtc gacacatgct gtgttcaacg atttattgct
     2461 aataccctca atcattcgta ctcacccccc gctgttttga atttgatccc cgctgcctgc
     2521 aatgcgtgct cgacgtcgaa gcggtccagc catccaccaa agtggtgcgg catcattacc
     2581 acccgctcac cgctatttat tgggtggccc atacggggtt cgtagccatc cggtaactcg
     2641 gcttccctcg cttccagttc tgcaattcgg cacatagcat caatatttgt gtcctccagg
     2701 cgcttaattt catccagcag cgccagtacc accgacggtg tgactttcat acgaaatgcc
     2761 agcaattttt gaggcgttgc tactgtttca attgctactg ctgcatcacg cagtgcctgg
     2821 taattaattt cgctcactgg ttgcctcctt tgtggagctg cgctgcgatg cacgaaaaaa
     2881 aagattcccg cgtatgacag ttaagagctg gtgcgaaagc cgcgttaaga acggcagcat
     2941 cacagccgtc atcgatatag agcgcaattt ttttctccag gcgcgctttg gcttcctgca
     3001 actgcatacc ccggcacgta cgcgggatat actcagcaat ttgagcgata gatttttcgt
     3061 tctgtttaaa catgcttcac ctcgacaggc ttgattgtgt cgatcagcag tctgcggcgc
     3121 gtattttctg caaagtggcg gcgtccggtt tctttgtggt aaaactcgtt tttgccgacg
     3181 acccacatcc gctctgtctg gtgaagtttt tttacctgcg ggccgtcttt ggtgatcaca
     3241 atgccggtat gggtttttac gattgtcatg ccactacctc ttcgaatttc aactccaatt
     3301 ggtcacccca gatttcacat gattcggaac acgaaccagt atcaaaccgc ttggccagca
     3361 ccatcgcttg atacaaattg ctgtagtcgc tgtcggcata catcctggca atcccgtcaa
     3421 gcgtcaggtg accacggtac ataacgtctt tacctgttct gcgatgacca tccctgacgt
     3481 gtttgcctgt aaccagttca ttaaaaaccc gcatcagacc aggttcgtct ttacatgcaa
     3541 gccccagctt ttgcgttgac tttttgatgc agaaaacaca gttcccgagg tgctccggga
     3601 tttgcaaatc aaaaggttgt tttcgccacc accggataac atccgactta tcaaaatctg
     3661 acagttcggc aagataccgg acgcccggtt tcggtttcag cctacggggt tcgtctgcac
     3721 gaatacccag ccacgtgatg tagttccctt gtccgaaatg gtcatcgcag tattttgtga
     3781 agggggtgag ttttaatctg tcagtgcaga acgcgccgcc gatgtatggc gtgccatatt
     3841 tttttaccat gtccataaac ggtttaagca ccggcattcg cgtctgaata tcctttggtt
     3901 cccattccgt ataaccattt ggctgcccaa gctctggatt tatatcgacc tgcaacacgg
     3961 ttagcggaat atcccagaac ttcacaacct cccgaataaa tcggtacgtc agcgggtgtt
     4021 cacatcccgt atccatgagt atgtaatgaa cgttttcacc agcttttctg cgttgctcca
     4081 taaggtggac gaggtacgcc gatgtccgac cgcctgaaaa actcacaaca tgatgagtgc
     4141 tcatttcttc gtctcgttac ggggaggggg ttaaataggt ggtaagctgg aattgccgga
     4201 tgtttacccg tgtccggggt acgacctcac gttgcagcgt acgagaatgg actattgctc
     4261 agtgtcgttt gatgtcggct gaggatattc gccttgctct tgtaacagag ttagaagaat
     4321 ggaaaacccc tcacgatgtg atgattgatg agtgtcgatc ccggaatgat tgatgttgtt
     4381 aaactccaac atcgtgctcc tggcgatagc ctcaacggta ttccagaata ctaatacatc
     4441 tgttttctgc tctgttttat cagcaacagg aagtttgata ttttgttgac atttattaat
     4501 gcaaccattt tgaatatgtt cctcgtgctt ttcattgtca taagcataca gcccgacaaa
     4561 aacatttcgc acgttacggg atatattttc cagattcgcc tttggttcct ttgttaaatc
     4621 aagcaccgat aaatgggctt taagcgtatc cgcagctatt ttctgaatat cctctggtaa
     4681 atctttaaat tgcatagtat accctccagg aactgtgtat tttctcgtga aatatgagcg
     4741 atatgtcgtg acgtccctgt cacagatttc atcacactga gaacgtgccg atgaaggtta
     4801 agatatcttc ttcttcgaaa cgctctttca gcaggtcacg gaactctttg gcaatttctt
     4861 cttccgcagc ttcaacacgc acgatacgta gcaccagaac aggttgttcg cttgtcagaa
     4921 tgcttaatcg cagcgtgaat tccctgttgc ccaggccctc gtatgggatg cattcaaagc
     4981 ggaaagtggc aggcatgatt tctttgcttt tagcctccac ggactccatg actgagcggc
     5041 taccgctgaa gttttgctct tcatattcag cactgcgttt tgcgtcgatg gtaagacgac
     5101 ggatagcaga gatagcctgt ttgatatcca gcacattacc gtcgctgtca taggctgtga
     5161 ggaagtcggc ccagtcttcc agccattcag cgagagattt ctggagttgt cttttgccgt
     5221 ccacgtcacg taatgcgcga tatggtgatg tggctttcag ttccagcagt gccttgttgt
     5281 cagcgtgtcc aggttgctcc agagtgccga tattgaacac agtgactgct ttcatcgttt
     5341 ccgcgttaat gaagcagcgg gaatgctcat cggcataccc tttgctgtat tcaacaaagt
     5401 catcaatact ggttgtggtc atgactccac ggaaacgaaa acgttctgtc atgaatcgtt
     5461 ccagactttc gatatttaca ttgtccggca ataacgcaac agggcattcc gtaagcggca
     5521 aattttcacc agaaaaagca gtagtggtga gttttacaat ttctttaatg gcattgctat
     5581 ctaactgaga catgtatatt tacctttaat ttatggattt agggataatg cgcgagcgtt
     5641 ttaaggcggg gattattcag cgtaaaacag cgtcatgttg accctgttta tcgaaaagct
     5701 gtccctggtc tttctggaac aaagtaagct caccaccttt atttacaaac atcggggtgt
     5761 tagttgtgtc ttcttctgat ttactaccac gcattgtcgg gcgtgtgaat tttagtttat
     5821 gggaaactga tacgcgattt tcatcaagtg aagataattc gaattcaaca catacctttc
     5881 cttttttgtt tgtgctgttt acgccaaagg caacttcact taataccgca ccaagtttgt
     5941 taacaaatac cccgccgtcg aggtcgttaa tgaaaacatt aacatcagtt tgatgctggt
     6001 tcatattaat atctccgtgt agaggtgtgt taaggcttcg gttgttcact gcatttaaag
     6061 cctggttttg tttgtgaatt aaaaactatc gctgccgtac tgccagtaac ctgggtcgtc
     6121 cgtgtgattc gggtttacgt tttttctgcg attgtctttg tctttgagag tggaaaccat
     6181 atttgctacc gtttctggtt tctttccttc agctttttct ttcagtgtct gccctgtttc
     6241 tgcaatgaat ggaatgcgga tttccatctg gtaaccgtct gcgccagtct ttctgtttgt
     6301 ggttaatact ttctggagga cgagtccgat tttctttccg tgaaactcag gtgcaacata
     6361 tttactgacg gaaaccatat gttgcgttaa ctgcccaatt cctgtgcatc ccatcatggc
     6421 gtgaataatg ctcgccccaa atttattttc agtgccgtca ttcttctgaa cacaaacact
     6481 gagatactgg attttccgtc cgtcgtcgga ttctccagaa aactcaatga atcgggcacc
     6541 tttttctgat tgcttgagtt ctgcttcggt gatgttaatg atatgcgcac ccgtttcggt
     6601 aataaaacca ccttgtccgg cggtcagtgc tgattcttcg ttataagtaa aaatcacttc
     6661 atccattagt tattaacccc ccattgttct ccaaatacaa aaccaatttc cgccagtgct
     6721 tcgtccattt tgtcgataaa ctccggcacc atctcatcaa aactcgccat gtacttttca
     6781 tcccgttcga ccacgacata atgcaggcct tcacgcttca tgcgtgggtc gtagttggcg
     6841 aagtaccagg cgtccttgtc tgtaacccac atgctgaatt gcacctgggc catgtaagca
     6901 ggcttgatag cgtcaaagcc gccaagccgg aatttcatga agtcgcggga agtaaaaggg
     6961 catttaagct caagaccgtt accatcgctg cataaaccat cgggagagca ggcggtgcgc
     7021 atgctttcgt cacgaaaaat tatgggtgtt tccgagacgg taacatccgc gataaattcg
     7081 aagagggcac gggcatcatc ttcgtattgc tttccccatg cgagcgactt agcgttaact
     7141 tccggtgcca cgccagtaca aacttcagcc agcagggtgt ggaagtatga catttttgta
     7201 tcaggccatt tgctgccgga acgtggcttt gctatcacat tgtgaacttc tgaagcagtg
     7261 ataacaccga gtctcagctt gtgccatcca tcatcgccct ggtcgaggct ggtaatatcc
     7321 acgccagttc gctggagaat aatttctggt gtcatgacac ggcctcgctg ttattttccg
     7381 tggtggcgtg aattttcgct tccgccgtgg ccttgtttct ggcagctttc tttttgacaa
     7441 aatcaagcgt tttgacagct tcttcctgac tgagatattc atgtgatgca atcgtgcgac
     7501 ggaatgtttt ggaacataag ggaagcaggt catcccatgt cttgttaatt tcggtgattg
     7561 cctgagtgat ctcattgata atctcatctg atgcgggagt gacgtcacgc tcagggatgt
     7621 gatcagcatt caggatgata ccttcaccgg cctgagtgct caggtagtcg atagctgtat
     7681 ccaggcgatc gcgacggggc cagtatttgc tggcgcgctt aacaatggct tttctggcca
     7741 tttcgtcggg gaagctatcc cacgggctac ttcctccgtt atttccggct ttgctgcacg
     7801 ccctgatgac ctcgatttcc ctgttgctca tctcttcggt cagatagtcg ccttcggatg
     7861 tttttacgac acaataaccg ccaatgcgtg cgcccctgtc tacgaatggg ttgtatttgt
     7921 gcgtgggggc gcagtcaata ccgttggact cgtaaatgtc cttctcgtaa acaagtttgc
     7981 attgccccca ctgaatggct cctgtgacct gtgccagatg cagaagcccc atataactga
     8041 tatccaggca tacagccttt tttcgtggga gcagatacgc cagcttgctg gccgggttca
     8101 gggttatgcc gatggcggca acattgatga tagcgttctg tgcgctgggc agatttgccc
     8161 gtgctgtgtc agccagaaag gcatttttct ggaattgctg aattgcaaac tgactttcct
     8221 tcgcccatgt cagcgtcggt tcagttaatg cctcgcagaa aaagcgctcc tgctgcttaa
     8281 caaattcaac gatatcgaac atttttttgg tcctgaaaat cagaaaggac agggggagaa
     8341 ttttctctcc cattcttctt ccgcccgagc ataggcgatc gctgagatat aatcgttgta
     8401 cgcctcttca gctttttcgc cagtgagtgc cagctgggct tctttgggta aaaaaaggct
     8461 gctcataagc aatggtttat cggggaacat gctgataagc tcctgcgccc gatcatcaat
     8521 ccatttatcc ttttcatcct gaatttgctg attaacccag cgacgctcct ctatgcggtc
     8581 gcaggtgagg tatgcgttca tggcggaact cctgattccg gttaatgcat taaattaatt
     8641 tgtcgggaaa gctgacagac agggcagtta cattcttcct cctgctcttt agcaaagaaa
     8701 tatgcagcgg cctgtaatgc gatgtcttct ggatgttccg cgataaacat aacattgcct
     8761 tccgtatcaa taacagaaat agcctcatca ggcaggacga caaaataggc gatgatttta
     8821 tcatccataa aaacttctcc cattatcgtt ttcgctggcg ttatgacgct ttttacattg
     8881 atattaattt cttgattgag catgatattt cctttcaggc tggtgagatt aacggttggc
     8941 ctttattgtt caggtaaact tctattgcat ctgagataat gcgaattttt tcaatcagtg
     9001 aatgtgcgta aagtgcatta ttaacgttag ctgacgccat gtaataacgc ccgttgtaaa
     9061 gaattgcagt gccgggttta atttcctcgc gagaaactaa tgcggttccg tagtgaggtt
     9121 tgagcatgac aaatcctccg gttaattaat ccagatattt aatttaatcc ccgatatata
     9181 atcggggaga gatttaatta aagattaacg ttgaaaccaa agcgggaaga tttttccgat
     9241 gtgcgggaaa tatccagcaa tttacggcgc atttcttccg tcagatgttt gaaggcgtta
     9301 aattcggcga cagcggcgtc aacgttatag cccttgctgt gtagctcatt gaggatgcgc
     9361 atagttgggc ttggcatatc gaagagcacg gaagcatcaa ggctgataac tttgcgatca
     9421 acatagttca tcgtggcgta ggggtgatgc tctgaaaacc acgataaagg gaaattgata
     9481 ttcatcgcag gagaagacag ggccagttgt ttctgttccc atagttgttt ttccatgcga
     9541 tcgaattcag caatgtaggc ttccttgaat gcagcggctt ttttaccagt gaatcccatc
     9601 accaggaaaa cgaatccgtt tttggtgatt tggtacattg ggcgttgttc gcctttggcg
     9661 tcggtgtagg taacgtcctc aaaattgagg gcgttaaatt tctctgagca ttcaatattt
     9721 gcaatggcac gtaatacgtt atcgtgtcgc ttgcaaaaat attcggcaac ggcaaaggac
     9781 gtagtaacag cgcggccatt ggaaatggtg atttcaggtt gagaaaggac tgggatagta
     9841 gccataatgg cagcctcctt gattggtgat taataaccac cgctggaggt gagaagctcg
     9901 ctggcggtgg actgtacagg gttctcacaa ctggcaatca agggaaccag cccgaccgaa
     9961 gtcggcccca tacagcccac cattgataag atgtgcgtgt atgttgatac aaaaaaagac
    10021 gctggcgcgt ctgtatcgcc tcgattgtca gcggggtgag aatcccgaca cccgttttat
    10081 aaggtgttcg ccaaatatag ccccgacatc acacgcagtc aataccgtcc tttctcagaa
    10141 acgctttggc ggtgccgcct gcaccccaaa acattccctg tattggtcag cgccaacttc
    10201 ctgccagtgt tgcccgttct cacgccgttc tcgctctcgc gcggggatac tctctcatcg
    10261 accggatcgc acccgatgat acagcacgtt tacgtgtagg ggtctaaaca ggtcattgac
    10321 gctgtaaagc tccagattgt taaagagcat tttgcggcgg gttaagtcgc gccgtacgac
    10381 tgatttatgt agccctgtgt aagggctcga tgtttctggc ttgggtaaaa tataataagc
    10441 gtaactgatc atagt
//
LOCUS       DAZKFU020000168        10400 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZKFU020000168 DAZKFU020000000
VERSION     DAZKFU020000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.169"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..468)
                     /locus_tag="ACHOQQ_RS17120"
                     /old_locus_tag="pgaptmp_003424"
     CDS             complement(<1..468)
                     /locus_tag="ACHOQQ_RS17120"
                     /old_locus_tag="pgaptmp_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HGU4471635.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAFQRPNVTNMRQA"
     gene            571..1341
                     /gene="yqeC"
                     /locus_tag="ACHOQQ_RS17125"
                     /old_locus_tag="pgaptmp_003425"
     CDS             571..1341
                     /gene="yqeC"
                     /locus_tag="ACHOQQ_RS17125"
                     /old_locus_tag="pgaptmp_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGU4471636.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQT
                     RGRRVLITTTTHMFMPKSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1389..3014
                     /gene="yqeB"
                     /locus_tag="ACHOQQ_RS17130"
                     /old_locus_tag="pgaptmp_003426"
     CDS             1389..3014
                     /gene="yqeB"
                     /locus_tag="ACHOQQ_RS17130"
                     /old_locus_tag="pgaptmp_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGU4471637.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLTTSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3235..4167)
                     /gene="arcC"
                     /locus_tag="ACHOQQ_RS17135"
                     /old_locus_tag="pgaptmp_003427"
     CDS             complement(3235..4167)
                     /gene="arcC"
                     /locus_tag="ACHOQQ_RS17135"
                     /old_locus_tag="pgaptmp_003427"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4471638.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4215..5600)
                     /gene="hyuA"
                     /locus_tag="ACHOQQ_RS17140"
                     /old_locus_tag="pgaptmp_003428"
     CDS             complement(4215..5600)
                     /gene="hyuA"
                     /locus_tag="ACHOQQ_RS17140"
                     /old_locus_tag="pgaptmp_003428"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGU4471639.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5653..6864)
                     /locus_tag="ACHOQQ_RS17145"
                     /old_locus_tag="pgaptmp_003429"
     CDS             complement(5653..6864)
                     /locus_tag="ACHOQQ_RS17145"
                     /old_locus_tag="pgaptmp_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGU4471640.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6922..8118)
                     /gene="dpaL"
                     /locus_tag="ACHOQQ_RS17150"
                     /old_locus_tag="pgaptmp_003430"
     CDS             complement(6922..8118)
                     /gene="dpaL"
                     /locus_tag="ACHOQQ_RS17150"
                     /old_locus_tag="pgaptmp_003430"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGU4471641.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVFSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8176..9363)
                     /gene="ygeW"
                     /locus_tag="ACHOQQ_RS17155"
                     /old_locus_tag="pgaptmp_003431"
     CDS             complement(8176..9363)
                     /gene="ygeW"
                     /locus_tag="ACHOQQ_RS17155"
                     /old_locus_tag="pgaptmp_003431"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGU4471642.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9842..>10400
                     /locus_tag="ACHOQQ_RS17160"
                     /old_locus_tag="pgaptmp_003432"
     CDS             9842..>10400
                     /locus_tag="ACHOQQ_RS17160"
                     /old_locus_tag="pgaptmp_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HGU4471643.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVN"
BASE COUNT         2458 a         2506 c         2710 g         2726 t
ORIGIN      
        1 tgcctgacgc atattggtga cattgggtcg ctggaatgcc tgcatcaagc atggttttga
       61 cactaaaatc ggatggccgg ggatgccatt atggagtggc agtattgcgc catcatttcg
      121 taacgaccag atttttctaa aaatatcaat ggtgagggtt ggcatgtcac cgtgggtgag
      181 aaaacaatgt tctgtttgca ctgcgggtac tgcggccttg actgatgtta gtaaaccctg
      241 ggcataatct gggttgtgaa taatagtgat attactctgg ttcgcataac gttcgtgcag
      301 ttcattacca cgatagccgg tgactaaaat aattcggcta caaaactgca acgcattttt
      361 gatacttgta tcaagaattg ttccctgttg ccagggtaac atcattttcc attgccccat
      421 tcttgatgat aatccggcag cggtaattat acagtcgatg gctgacatag tggttccatt
      481 atttataggg gctaataacg cctgataatt gcgttacttc ctgatggatt aagttggggg
      541 taatttatcc cagagaggtc ataaagactc gtgaaaagta tagttgaccc atcggcatta
      601 ttcattgatt taggtgcgca gaaacgtccc actgtgattt ctgttgttgg tgctgggggg
      661 aaaaccagct tgcttttttg gctggcagaa ctgctccaga ctcgcggcag gcgtgtgtta
      721 atcactacga cgacacatat gtttatgcca aagtcacact ggcccgtggt tttctgtcgt
      781 gatcccgcca tgcttcctca tgcgtctctt acatccccca tttcattttg ttttcacagc
      841 tggaaagcga accagggaaa agtgcaggga tttacgccag aagcgattga tgcactggtg
      901 caacgaccag agtgtgacgt aattctcatt gaagcagatg gctcgcgtgg aatgccgtta
      961 aaagcgcctg atgagcacga accttgcata cccaaaagca gttgctgcgt gattgctgtg
     1021 atgggaggac atactttggg cgcgaaagtg agcacagaaa atgtccatcg ctggtcgcag
     1081 tttgctgata ttaccgggtt aacacctgat gcaaccttgc aactgagcga tctcgttgcg
     1141 ctggttcgcc accctcaggg ggcgtttaaa aacgtaccgc aaggttgtcg gcgagtctgg
     1201 ttcattaacc gtttttctca atgtgagaat gcgattgcac aaagcgagct ccttcaaccg
     1261 ctgcaacaac atgacgtaga ggcaatctgg ctgggcgata tacaagaaca tcctgcaatc
     1321 gcgcgcagat ttgtgaatta gcggcccggg tattccgggt tactgaaagg atatccattg
     1381 aggttggtat gaatattttc acagaggctg caaaactcga agagcaaaat tgtccgtttg
     1441 cgatggcgca aattgttgat agccgaggct cgactccccg ccattctgca caaatgttag
     1501 tgcgcgccga tggttctatc gtcggtacaa ttggtggcgg aatggttgag cggaaggtga
     1561 ttgaagagtc gcttcaggca ttgcaggaac gtaagccgcg attattccat ggacgtatgg
     1621 ctcgtaacgg tgcggatgct gtcgggtcag attgtggagg tgcaatgtca gtatttatca
     1681 gcgtccatgg tatgcgtcca cgtctggtgt tgattggcgc ggggcatgtc aaccgggcga
     1741 tagcccagag tgcggcgcta ttaggatttg atattgccgt tgctgatatt tatcgcgaaa
     1801 gcctcaatcc tgaactattc ccaccatcaa ccacgcttct ccatgctgag tcgtttggtg
     1861 cggcagtgga agcactggat attcgccctg ataattttgt cctgattgcc acgaataatc
     1921 aggatcgtga agccctcgac aaactcattg aacagcccat tgcatggttg gggttgctgg
     1981 caagtcgtcg caaggttcag cttttcctgc gtcaattgcg tgagaaaggc gtggctgaag
     2041 aacatattgc ccgtttacat gcgcccgttg gttacaacat aggtgcggaa acgccgcagg
     2101 agatcgccat cagcgtgctg gcagaaatat tacaggtgaa aaataacgcg ccgggtgggc
     2161 tgatgatgaa accttctcat ccttccggac accagctggt ggtgattcgc ggtgcggggg
     2221 atatcgccag tggtgtggcg ctacgtctgt atcatgcggg ttttaaagtg atcatgctgg
     2281 aagtggaaaa accgacagtg attcgttgta ccgtggcgtt tgcccaggcc gtgttcgatg
     2341 gcgaaatgac ggtcgaaggc gtcactgctc gcctgacaac cagctctgcg gaagcgatga
     2401 aacttaccga acgcggattc atccctgtga tggtagatcc cgcctgttca ttgcttgatg
     2461 aactgaaacc gctttgcgtg gtggacgcta ttctggcgaa acagaatttg ggaacgcggg
     2521 cagatatggc accagtaaca atcgcgcttg ggcctggctt tactgctggg aaggattgtc
     2581 atgcggtaat tgaaacaaat cgcgggcact ggctcggtca ggtgatttac tctggttgtg
     2641 cgcaggagaa taccggtgtt cccggcaata ttatggggca taccacccga cgggtgatcc
     2701 gtgctcctgc tgcaggcatt atgcgatcca acgtgaaatt aggcgatctg gtgaaagagg
     2761 gcgatgtgat tgcctggatt ggtgagcatg aaattaaagc accgttgacg gggatggtgc
     2821 gtggcttgtt gaacgacgga ctggcagtgg tcggtggttt taaaattggt gatatcgatc
     2881 ctcgtggtga aacggctgat ttcaccagcg tttctgataa agcccgggcg attggcggcg
     2941 gcgtacttga agcgttaatg atgttgatgc atcagggcgt taaagcgacg aaagaagtgc
     3001 tggaagtggc ttaaatgaaa atggccggat ggtattaccc acccggcctg agactgatga
     3061 caaacgccaa attgcctgat gcgctacgct tatcaggcct acaagtgcat tgcaatctat
     3121 tgaatttgcg atgttttgta ggacggataa ggcgctcgcg ccgcatccag catcaacaaa
     3181 gcgcacttac ttgacaatct ttggccggat ggtattaccc acccggcctt aaaattactg
     3241 acaaatcacc gttccggttt tgccttcaat cccttctttc gctttgctca atacggtaat
     3301 cagtgcttca cgacctgcgc gagagcgagc aaacgaggcg gcagcttcca ctttaggcag
     3361 catagaacct ttcgcaaaat gaccttcttc gataaagcgt tcagcatcac ttaatgacag
     3421 gcgatcgagc cactgttcat tctctttacc gaagttaatg gccacttttt ctaccgcagt
     3481 cagaatgatc agcatatcgg catcaatcat ttctgctaaa cgggcgctgg cccagtcttt
     3541 atcgataacc gcgctggcac cacgcagatg gttaccttca cgaataacag ggataccacc
     3601 accgccaacg gtgatcacca cctggcctgc atctaccaga gctttaaccg tttctttttc
     3661 aatgatatca accggttttg gcgaggcaac aacacggcga tagccgcgac cagcatcttc
     3721 tttcagagtg taaccctgtt ttgtcagttg ctctgcttct tgctcggtaa agaacgaacc
     3781 gatcggcttg gtcgggttga ggaatgccgg atcgttagcg tcgacttcga cctgggtaac
     3841 cagcgttgct acaggtttat taatgccccg agaaagcagt tcttctctta aagcgttttg
     3901 tagatcgtaa ccaatataac cctggcttaa tgcaacacag acagacatcg gcagcattgg
     3961 cgagtgtgct tcagtttttg cggcagcttc aaaggcctgg ttaatcatgc ccacctgtgg
     4021 cccgttacca tgagtgacga taacttcatg tccctgggca attaaatcaa caattgcctg
     4081 agaagtaatt tttaccgctt tcatttgccc ggccaggtca tcgcccagcg cattcccgcc
     4141 cagggcgaga acaattttct tactcatttt tcctcactca tcattcttga ttgtgtatag
     4201 atccctggca gtggttagag cacgggaggg acaaacggtt tgcgtcgcag gaaacgacct
     4261 cgcccggctt tgcctgtaaa ggtgccgtca cagaaaatcg tttcaccacg ggataaggtt
     4321 ctgacaatcg cgccctgaca ggtaaaaccc tcccatggcg agtagtcggc gttgtcgtgg
     4381 agatggcgat gctgaatttg ttggctctga cgtgggtcga taatcaccac gtcgccatcg
     4441 gaaccgggcg ctaataatcc tttttgcggc cacaggccaa acagcctggc gggcatcgcg
     4501 ctggttaatt caacaaaacg ttccggtgtt atacgtcccg tcatcacgcc actggaaaac
     4561 agtaactgca tgcggttttc cacaccgggt aagccatttg ggcagcgact gaaatcgcct
     4621 ttagaaattt gcaggcgttg agccattgag aacgtgcagt gatcggtcgc caccacgtca
     4681 atcgcaccat cgctgatgcc acaccacagt ttgtcctgct cgcgtacgtt acgcagcggt
     4741 gggctaagaa tgaacttcat gccatcttct gtatcgtagc tgcgttcgtc caacaggaga
     4801 tattgtgggc aggtttcaac ccagactggc tggtgattcg cacgggcaag acgcagataa
     4861 tccagaccta agccgttaga caggtgcacg atatagagcg gggcgttacc ggcaatttgt
     4921 gccaggttaa tcatgcgggc gatggcttcc gcttcgcatt ccagagggcg actcaaggcg
     4981 tgatagcgcg gcgcggttaa ccctgcggcg ataaactccg cccgcttgct ggcgatagca
     5041 gcatcatttt ccgggtgcac ggtggtcagc gcgccggatt catgcaagcg gcgtaatgcc
     5101 tgcaaaactt cgtcatcgtt gagtttgtat tgataggtta aatagagttt aaaactgctc
     5161 agtccttcct cgaccatcat cgggatttcg tcgaggatgg cgtgattaat gtgctggatc
     5221 acaccgtgaa agctgtagtc gatgaccgcc ttatgggcgg catagccacg ataaacctcc
     5281 agttgatggc gtaaccgaca gccgtttggg ccaaatccca tatggtcaat aatggttgtt
     5341 gtaccgccac acgcagctgc gcgggtaccg gtaaaaaaat catcacaact gcgcgcgatg
     5401 ccgacatcaa tattgaaatg cgtatggaca tccacgccgc cagggaaaac gtaacagcca
     5461 gtggcatcaa tttcttcata cgggagctgc ggcgaaatat tgttgcccaa ctggcgaaca
     5521 atcccgcttt caatcagcaa atcctgtttg gcttgtccat ctgcgttaac gacagtgccg
     5581 tttttgatca atacgcgcat agcaaactcc aaaaaccctc cggcgaaccg gaggggaata
     5641 agtgaagagt aattattcgg ttgccagcca gcttaacggg attgcagcgt acatcgcggc
     5701 acaggtcacc aagtgagatt tccaggtttt ttcgttaggt gcatgcgctt caggttcttt
     5761 acccgggcca aagccgatca ccggaatgcc gtgacggccc atgatagata cgccgttagt
     5821 tgagaaggtc cacttatcaa caaccggcgc tttgccaaac agcccttcgt aggcattcac
     5881 cagtgcttta acggtgaagt gatcttcttc cactttccag gtcgggaagt agcattcggt
     5941 tgggtaaacc aggccagtcc aggacggacg gtcgtagttg tacatagaaa caaccgcgtt
     6001 agctttctgt actgcaggca gggcgcggat ttcgtccagc gcgccttccc aggtttcgcc
     6061 ccaagtcaga cggcggtcaa tagagacggc gcagctatct gctacagcgc aacggcttgg
     6121 ggatgtgaag aagatttcag aaacggtgag ggtgcctttg ccgaggaatt cgtcataacc
     6181 cagacgttgg gagagttctt gtaattcgcc aagaatcgga cccattttga aaatggcgtt
     6241 gtcaccgcgt tctggcgcag aaccgtggca gctaacaccc tgaacatcaa tacgaatttc
     6301 catacgaccg cgctgaccac ggtatacctg gcagtcggtt ggttcggtac tgaccacaaa
     6361 ttccgggcga atgccggatt gttcaataat gtactgccag cacagaccgt cgcagtcttc
     6421 ttcctgaacc gtaccggtaa ccagcagggt atattcatct tccagaccga ggtctttaat
     6481 gattttaccg gcataaacca tagatgccat gccgccttcc tggtcggaag taccgcgacc
     6541 accgatcagc tcatcagttt ccatgccttc gtacggatcg aagtcccagt ttttgatgtt
     6601 gccaatgccg acggtatcga tatgagcgtc cattgccacc agacgcgggc cgtggccgat
     6661 ataaccgaga acgttgccca tcgggtcgat ttcaacttta tcgaagccga ctttttccat
     6721 ctcttcttta atacgatgta ctacgcgttt ctcgtcgcag ctttcactgg gaatagcaac
     6781 catgtcgcgc aggaagcgag tcatgtccgc ctggtaatct tttgcttttt caagaatcag
     6841 tttgaatgga atattcttag ccattatttt tctccagatt ttttactccc cgttttgcag
     6901 ggagtagttc caatagggtg attaaggtgc tacagcgtgt ttgccttccc agacaacttc
     6961 gcggtagtgc ttcacgtcgg tgtcgccttc agtgctgata accagcacca cggcatcttt
     7021 gttcagcgcc agtttttcca tcaggctttg acgttgcggg tgataatgaa ccgccgcgag
     7081 aacgcccaaa ccgacagcgc cggattcacc ggagatgatg cgcgggtcgt tgccgtacgg
     7141 attacccagc acgcgcatac ctaatgcggc aacgctgtcc tggcaggaga tgaattgggt
     7201 ggcgcagtta cgtaggattt cccagcccag cgggttaggt tcgccacagg ccaggcccgc
     7261 catgatggtg gccatatcac cgccaacgtt gacgatgtcg cctttgacgc cggagcgata
     7321 aatacagtca gctttgtcag gttcaacaat aatgctgtgc agattttgcg ggctaaagac
     7381 gtcgaccaga taacccagca caccaccggc cattgctccg acaccggctt gcagcagaac
     7441 gtgcgtcggg gttacgccca tttcacgcat ttgctcgacg gcttcatctg ccagggttgc
     7501 gtagccttgc atgatccagg ttggaatttt ggtgtaacct tcccatgccg tgtcctgtac
     7561 cacttcccag ccgtgctgct gcgcgtgttg catggtcagg cgaacggtat cgtcatagtt
     7621 catatccgtg acgatgcact cggcaccgag gttcagaatg gcgtcaacgc gttcctgagc
     7681 agaacctttc ggcatgtaaa tcaccgcatt ctgtccgagt tgctgcgctg cccacgccac
     7741 accgcgcccg tggttgccgt cggtggtggt cgcgaaagtc attttttcgc cgatggcatt
     7801 tttcaggtgc tcaaatgaca gcgtttcgat atcaagatga tatttttcac acaataattg
     7861 agcgatagcg tacgcaccgc cgagcatttt gaaggcgttc agaccgaatc gttttgattc
     7921 gtctttgaca agaattttct tcacaccaaa aaggtttgcg agatcgtcca gcgcacaaag
     7981 cggtgttggg cgataaccag ctattttctg gtggaactgg cgcgccagtt tggcctggct
     8041 ttgcgaaaag agcggtgatg tttcgccgtt gaaaaatttg ttatcggcga tatcaatctt
     8101 caatgagaaa acggacatat cctatccttt tggcctctgg aaatagaggg ccgaccagct
     8161 gatcggcccg aggggttatt tcacgcgttc ttgcgcttct ttcagcagtt gctcgagcag
     8221 tgcacctggt ttggcgtatt tacgggacag gatcatcgcg gcgatgatat aaggcttcca
     8281 gctggcttct ttgtaggtag cgatacggta tttttcgaat acgccttcag tcacttcacc
     8341 ttctttacag gatacgccgc tgatatcagc tggcaggcag tgcatgtaca gggcttcgcc
     8401 atcacgggtc agttccatca tctcttcagt acaatgccag tctttgtgtt gcgcgttctg
     8461 tgccagacac tgtttttcca gtgcttttaa gccttcgtga tcgttcgcac gcagcaattc
     8521 agtacgctct tccatcactt tgtaaggtgc ccatgacttc ggataaacga tgtctgcgtc
     8581 tttgaaggct tcttccatgc tggtgacctg acggaagcta ccgccggagg ctttagcatt
     8641 gtttttagcg acttcaacga catccgggat caggtcgtag ccttccggat gggccagggt
     8701 gacatccata ccgaagcgag tcatcagacc gatgatgcct tgtggtacag agagcggttt
     8761 gccatagctt ggggagtagg cccaggtcat ggcgattttt ttacctttca ggttttccaa
     8821 tgaaccaaag tgttcacgta accatgccag gtcagccatt gactgagtcg ggtggtcaat
     8881 atcacattgc aggttcacta aagccggacg ttgcggcagc acaccctgct tgtaaccgtc
     8941 atcaagtgca gcgccaactt cacgcatata ggcgttgcct gcgcccagat acatatcgtc
     9001 gcgaatacca atagcgtcgg cgcagaagga gatcatattg gcggtttcac gcacggtttc
     9061 gccgtgagcg atttgtgatt tgccttcgtc gagatcttgc tgtgcaaggc cgagcaggtt
     9121 aagcgcggaa gcataagaga agcgggtacg ggtggagttg tcgcggaata cggaaatacc
     9181 taatccacta ttaaagactt tggttgagat gttttcagca cgcagtgctt ttaatgctgc
     9241 ggcaacgtcc agtacttgtt tcagttcatc tggcgtctgt tcccacgtta acaaaaaatc
     9301 tttctcgtga aggtgagagg tcagcgaatt gatatcctta atcagctcat taacagtttt
     9361 catcataaag tcccggtagt ggaatgacag gcaaagtggt ttatttcact gtgacttgcc
     9421 aaagtgattg gtgttttcac agctatacct gggactaaca ataagtgtgc cagaattatc
     9481 cacggggctg caaaagacta atttgacgga taatgtgtgg ggttgatcac aaattgatgc
     9541 tagcttccgg ttgtttgtaa aatacgaaaa cgtttggctt tttttattgc gttatttatt
     9601 tgctgctaag tatcagaaat aagataaagg ggcaggctat ccctcgtcag tttgataaac
     9661 tcaccgccgt aattctcata ttgatataga aatgtgatat ctcttccata atttcattag
     9721 aaagtgataa aaatcttatg tttttatcaa agccaatttc gcctgagtta atatcatcat
     9781 gatctgtgaa ccatcaacgt cttcaatgat cgacttttaa gtaagtctgg aaggtaaagc
     9841 tatggagctt gctactacgc agtcagtatt gatgcaaatt caaccgacaa ttcagcgttt
     9901 tgccagaatg cttgccagcg ttttgcagct tgaggttgag atcgttgatg aaaacttgtg
     9961 tcgcgtcgcc ggaactggcg cgtatgggaa gtttcttggt cgccagttga gcggcaactc
    10021 acgcctgctc cgccacgtcc tggaaacgaa aactgaaaaa gttgtgacac agtctcgctt
    10081 cgatcccctt tgcgaaggtt gcgatagtaa agaaaattgc cgcgaaaaag catttctggg
    10141 tacgcctgtc attttacagg atcgttgtgt tggggtgata agtttgattg ccgttaccca
    10201 cgagcaacaa gagcatatca gtgataattt acgcgaattt tctgattatg ttcgccatat
    10261 atccaccatt tttgtttcga aacttctgga ggatcagggg ccaggagata acatcagtaa
    10321 aatattcgcg accatgatcg ataatatgga tcagggcgta ttagttgttg atgatgaaag
    10381 tcgggttcag tttgttaatc
//
LOCUS       DAZKFU020000169        10292 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZKFU020000169 DAZKFU020000000
VERSION     DAZKFU020000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.170"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(116..922)
                     /gene="trpA"
                     /locus_tag="ACHOQQ_RS17165"
                     /old_locus_tag="pgaptmp_003433"
     CDS             complement(116..922)
                     /gene="trpA"
                     /locus_tag="ACHOQQ_RS17165"
                     /old_locus_tag="pgaptmp_003433"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGU4471644.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(922..2115)
                     /gene="trpB"
                     /locus_tag="ACHOQQ_RS17170"
                     /old_locus_tag="pgaptmp_003434"
     CDS             complement(922..2115)
                     /gene="trpB"
                     /locus_tag="ACHOQQ_RS17170"
                     /old_locus_tag="pgaptmp_003434"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGU4471645.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTKTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIEIGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2127..3488)
                     /gene="trpCF"
                     /locus_tag="ACHOQQ_RS17175"
                     /old_locus_tag="pgaptmp_003435"
     CDS             complement(2127..3488)
                     /gene="trpCF"
                     /locus_tag="ACHOQQ_RS17175"
                     /old_locus_tag="pgaptmp_003435"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGU4471646.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNIEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3489..5084)
                     /gene="trpD"
                     /locus_tag="ACHOQQ_RS17180"
                     /old_locus_tag="pgaptmp_003436"
     CDS             complement(3489..5084)
                     /gene="trpD"
                     /locus_tag="ACHOQQ_RS17180"
                     /old_locus_tag="pgaptmp_003436"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGU4471647.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5084..6646)
                     /gene="trpE"
                     /locus_tag="ACHOQQ_RS17185"
                     /old_locus_tag="pgaptmp_003437"
     CDS             complement(5084..6646)
                     /gene="trpE"
                     /locus_tag="ACHOQQ_RS17185"
                     /old_locus_tag="pgaptmp_003437"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGU4471648.1"
                     /translation="MQTQKPTLELLTCEGAYHDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LSFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6668..6761)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6738..6782)
                     /gene="trpL"
                     /locus_tag="ACHOQQ_RS17190"
                     /old_locus_tag="pgaptmp_003438"
     CDS             complement(6738..6782)
                     /gene="trpL"
                     /locus_tag="ACHOQQ_RS17190"
                     /old_locus_tag="pgaptmp_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGU4471649.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6920..7801
                     /gene="rnm"
                     /locus_tag="ACHOQQ_RS17195"
                     /old_locus_tag="pgaptmp_003439"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6920..7801
                     /gene="rnm"
                     /locus_tag="ACHOQQ_RS17195"
                     /old_locus_tag="pgaptmp_003439"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGU4471650.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDIIHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7798..8418
                     /gene="yciO"
                     /locus_tag="ACHOQQ_RS17200"
                     /old_locus_tag="pgaptmp_003440"
     CDS             7798..8418
                     /gene="yciO"
                     /locus_tag="ACHOQQ_RS17200"
                     /old_locus_tag="pgaptmp_003440"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGU4471651.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8446..>10292
                     /gene="yciQ"
                     /locus_tag="ACHOQQ_RS17205"
                     /old_locus_tag="pgaptmp_003441"
     CDS             8446..>10292
                     /gene="yciQ"
                     /locus_tag="ACHOQQ_RS17205"
                     /old_locus_tag="pgaptmp_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGU4471652.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLQFSNIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTLGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQSDKNQQTLIRV
                     NEGVTRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFTR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWVARITPSLTAAVSAPEIARSGVLFSLQMHLSLGANTSLLGRSYSG"
BASE COUNT         2406 a         2658 c         2717 g         2511 t
ORIGIN      
        1 acgttcgaca ggggtaagcg aaacggtaaa aagataaata ttaaatgaat ttaggatttt
       61 tccggcttcg ttaaagaaag ttaaaatgcc gccagcggaa ctggcggctg tgggattaac
      121 tgcgcgtcgc cgctttcatc ggttgtacaa aagctttcag tgccgccagc attttctctg
      181 gctcattaat atgttgctcg atgattttaa caatggccga accagaaatc gcgcccgcag
      241 ctcctgcatc aatcgctgct tttacctgat ccggggcgga aataccaaat ccctgcaatg
      301 gaggtgcagc gttgtactct ttcagcttcg caaccagatg attgaggggt aacgcggcgc
      361 ggttttctgc gccggtcacg cccgctcgtg acagcaaata tgtgtaacca cgaccgtaag
      421 aggctatctg gcgcagcagg tcgtcatcgg catttggcgg gcagatgaag ataggtgcga
      481 cattatgacg caacgcggcc tggcggaagg gcgcggactc ttcaactggc acatcggcaa
      541 ccagcaccga atcgacgccg actttttcgc actgggcata aaactcatca atgcctttgt
      601 taaacaccag attggcatac atcaacaggc caatgggaat ggtcgggtgt ttctggcgaa
      661 tcagtgccag catttcaaaa cattgtgccg gagtcacacc tgccgcaaag gcgcgcagag
      721 tggcgttttg aatggtcggg ccatccgcca gtgggtcgga gaaggggata cctaactcca
      781 gcgcgtcagc accggcttca attagcgtat cgataatttt caatgactgc tcaatgcccg
      841 gatccccgag cgtgacgaaa ggaacgaatg cgccttcttt gcgctccttc aactgggcaa
      901 acagagattc gtagcgttcc atcagatttc ccctcgtgct ttcaaaatat cgtgaacggt
      961 gaagatgtct ttatcgccgc gaccggaaag gttaaccacc agtagctgct ctttttccgg
     1021 gttttcgcgc atcattttca gtgcatgggc cagggcgtgg gaggattcca gcgccgggat
     1081 gatcccttcg tgcaggcaca gcgttttgaa ggcttcaagg gcttcatcat cggtaataga
     1141 cacgtaatca gcgcgtccag tgctgttaag atacgcgtgt tgtgggccga cggacgggaa
     1201 atccagcccg gcggagatgg agtaagattc ttcaatctgc ccgtcttcgg tttgcatcat
     1261 cggcgctttc ataccgaaat agatgcccac gcgaccatgt tttaacggtg cgccgtgctc
     1321 gccgatttct ataccgtgac cgccaggctc cacaccaatc aggccgacgt tggtttcgtt
     1381 gatgaaatct gcaaacatgc cgatggcatt cgaaccgccg ccaacacagg cgataacggc
     1441 atccggcagg cgaccttctc tttccagaat ctgtgctttg gtttcttcgc caatcatccg
     1501 ctgaaactca cggacaatgg tcggataagg atgcgggcca gctgcggtgc ccagcatata
     1561 gtgcgcggtt tcataactgc cggaccagtc gcgtagcgcc tcgttacagg catctttcag
     1621 cgtcgcggaa ccgctatgca ccgggatcac ttccgcaccc attaagcgca tacgaaaaac
     1681 gtttggcgac tggcgttcaa cgtctttggc ccccatataa atacggcatt tcaggccgag
     1741 cagggcgctg gcaagggccg acgccacgcc atgctgaccg gccccggttt cggcgatgat
     1801 ttcggtttta cccatccgct tcgccagtaa agcctgaccg agcacctggt tagttttatg
     1861 cgcgccgccg tgcagcaaat cttcgcgctt cagatacagc gtggttttcg tcccagccgt
     1921 aatgttctgg catttggtca gtgcggttgg acgcccggca tagtttttca gtaggtcgtt
     1981 gaactgggcc tgaaattcag gatctttttg cgcgctgaca aaagcttctt ccagctggcg
     2041 cagagcaggc atcaggattt gtggcacgta catgccgcca aactcaccaa aataggggtt
     2101 aagtaatgtt gtcattgttc ctttccttaa tatgcgcgca gcgtctggaa aaccgaggcc
     2161 aaaagacgtg cgtctttgat gcccggttgc gactctacag cagaattaaa atcaagtccg
     2221 gcgcagccgg tttgtgccgc ttccacgcag ttatctgcgc ctaagccccc cgccagcaga
     2281 acgttgccaa gcgattgacc gtttaacagt gaccagtcga aacgttgccc gcttccaccc
     2341 tggccgttgt ctaaaacata tttatcaacg tgctgaagct cgcgggcggg cagggtttca
     2401 ccgacgctta atgctttcca gatggcaacg tgtgctggca gagcttcacg cagcgtatcg
     2461 atatacagct gatcttcatt accatgcagt tgcactgccg ccagcgataa caccttagct
     2521 ttgtccacca catcggcaat atcgtgattg cggaacacgc caacatactg caacggtgcc
     2581 gcagccatca cttcctgcgc ctgttcaatg ttgacgcaac gcggtgatgt cgcaacaaaa
     2641 atcaacccac cgtaaatcgc gcccgcgtca tacgctgctt tagcatcttg cccacgcgtc
     2701 aggccacata ctttattctc acccagcaac acccggcgca cggcggcgtt caaatcgtca
     2761 tgggccatca acgccgaacc aatcagaaaa ccgttagcga agtggcttaa ctcgcgcacc
     2821 tgagcgtaag tattgatgcc tgattcgctg attaccgtca cgttgtgccc cagtttcggc
     2881 gcaagctcgc gggtacggtt gagatcaatc gacaaatcac gcagatcgcg gttgttgatg
     2941 ccaacgacct ttgcccccaa tgcaatggcg cgctccagtt cctcttcatt actgacttcg
     3001 gtcagcacac ccatctccag actgtgggcg acggcggcaa gctggcgata ttgttcgtca
     3061 tccagtactg aaagcattaa taagcaggca tcggcctggt aatagcgcgc cagatagatc
     3121 tggtaagggt cgataatgaa gtctttacat aaaatcggct gcggggcgat ttggctgacg
     3181 atggggagga aatcaaagct cccctgaaaa tatttctcat cagtcagcac cgaaattgcc
     3241 gaagcgtaat gtttataaat ggcggcaatg cgtgctggat cgaaatcatc acggatcacg
     3301 ccttttgacg gcgacgcttt cttgcactcc agaataaacg ccgtgcgcgc accctgtagc
     3361 gcatcataaa aatgtcgcgt gctcggctga acctcattct gaaaactggc cagcggttgc
     3421 tgctgtttgc gggcttctac ccaaatcgcc ttgtctgcga cgatcttcgc taaaacggtt
     3481 tgcatcattt accctcgtgc cgccagtgcg gtgactctgt cgtaagcgga accactgcgc
     3541 agtacctcaa gaacggtttg cgcattggct tgcagatctt catggccatg caggcgcatt
     3601 aacatggcga cgttcgcagc gacggctgct tcatgggcgg cgtcgccttt accttgtaac
     3661 aaacgtgtta aaatgtcacg gttttcttcc ggtgttccgc ctgccagttg ctcctggtgg
     3721 tagggcgtca ggccaaaatc ttcagcggtc aattgatagc tcttaatttc gccgtcatgc
     3781 agctcggcaa cgattgtcgg cgcgtgtaat gaaacttcat ccatcccgcc gctgtgcacc
     3841 accgccgcgc gttgataccc cagcacgcgc aaggtttcgg caatcggcag caccagttcc
     3901 ggactataaa caccaattaa cgccagcggc ggatgcgccg ggttaatcaa tggtcccagc
     3961 acgttgaaca aggtgcgggt tttcagttgc tggcgaaccg gcatcgcgtg gcggaatccg
     4021 gtgtgatact tcggcgcaaa gaggaaacag acgcctaatt cgtccagcgc ctggcgcgat
     4081 ttatcggcgt tcatatcaag attaataccg aatgccgcca gcagatccga cgagccagat
     4141 ttactggaga cgctacggtt gccgtgtttc gcaactttca gcccacaggc cgcggcgaca
     4201 aacgcactgg cggtggaaat attgatactg ttgctgccgt caccgccagt cccgacgata
     4261 tcagcaaaca gataatccgg gcgcgggaac ggcgctgcgt tttccagtag cgcggttgct
     4321 gctccggcga tctcgttcgg gtgctcaccg cgaattttca tgctcaccag cgccgccgcc
     4381 agttgttccg gcttcagctc gccacgcacc accgctgaaa acagctggtg actttcttgt
     4441 tggctaagcg tctgcgcctg atacagtttt tccagaatcg gttgcagcgt gttggttggc
     4501 tcaagtttct gctgcgccca ggccagcgtt tgttccagca ggcgagcgcc ctgggtagta
     4561 agaatggatt ccggatggaa ctggaatcca caaacgcgat ccgcatcgtg acgtactgcc
     4621 atcaccatgc cattaaaatg ggcattgatg gttaaacctg ccggaatgtt actgccaacc
     4681 agcgagtgat aacgcgccac cggcaatggg tttgttaatc cggcaaacat cgcctgaccg
     4741 tcgtgttcaa tgctggaggc tttaccgtgg agaatttcgc ccgcctgacc gacatagccc
     4801 ccgtaagctt cgacaatcgc ctgatgacca aggcaaatgc caataatcgg cagcttaccg
     4861 cgcaggcggg taagcagttc tggcatacaa ccagcttcgc tcggcacacc gggtcctgga
     4921 gaaagcatca gcaccggatt gctcatcgtc gccaggcgtt caattaaggt ttgcgccgga
     4981 atatggttgc ggtaaatcac cacgttatgc ccattgctgc gcaactgatc tgccaggttg
     5041 tacgtaaaag agtcgatatt atcgagcagc agaatgtcag ccatcagaaa gtctcctgtg
     5101 catgatgcgc ggtggcaata gcgcgcagta cagcgcgggc tttattacgg gtttcgtcgg
     5161 cttccgactg cggaacagaa tcaaggacta cgccagcacc ggcttgcacg gtggcgatac
     5221 cgttttccac cagcgccgag cggatcacaa tgcaggtgtc gagatcgcca tgcgcggtga
     5281 aataacctac cgcgccgccg tagctgccgc ggcgacgacc ttcagcctcg gcaattaact
     5341 gcatagcgcg tactttcggc gcaccgctta acgtacccat attcatacag gcgcggtaag
     5401 cgtgtagggc gtcgagatcg tggcgcagct caccgaccac gcgggagacc aggtgcatca
     5461 cgtaagagta acggtcaact ttggtaagat cggcgacgta gcggctgccg ggggtgcaaa
     5521 tgcgtgccag atcattacgg gcgagatcca ccagcatcag atgttcagaa agctctttat
     5581 gatcggtacg catttccagt tcgatgcggc tgtcgaggtc tctgtccagt gaaccatcgg
     5641 cacgacgacc gcgtggacgt gttccggcaa tcgggtagat ctcaatctgg cggctggtgg
     5701 cgtcatactt gagcgaactt tccggcgacg cgccaaacag ggtgaaatca ttatcctgca
     5761 taaaaaacat gtacgggctg ggattactct ttttcagcac gtaataggcc gccagcggtg
     5821 acgggcaggg cagagagaaa cggcgagatg gcaccacctg gaaaatttct ccggcgcgaa
     5881 tcgctttttg caacaaacgc actacgccac cgaactcttc atcgctctgg ttacattcac
     5941 aacgcatatg cggcacggaa accaccggca gcggtggcgc ggtttcggtc agttgctggc
     6001 gaagatcgtt caggcgagca gtgagacgtt gtttttcttc ttcattcgga gcaaaaaggc
     6061 tggcctgaat gcgggtgctt tttttctgat ggtcaatcac catcagcgtt tcagcgagat
     6121 aaaaacagaa atcagggcag ttattttccg ctgacagttg cggtaaatct tcaaaccccg
     6181 ccacaaggtc ataagagaac aggccgccga agaacatggc ttctcgttct tccttcggta
     6241 cattcaacag attctgcaat aagcggaaag cgtcaaaaac cgaaagggag cataagcgag
     6301 cgtcttcatc cagcagtgga ctaacagggg ggaagctcag cacacggcag tttggtaatt
     6361 gttcattttc cacacccgca ggcagggcgt tatccagtag tgtcagcagg gcttcgccgt
     6421 tgccggaaag ggactggatt gtgacagtgt cacctaaagc tgtaatgcgc agcgcactgt
     6481 ctaccagcag caggcttttt aaatcatctt tgctgtcgat atctgcggat tccagcagca
     6541 gcgttgccgg acgattccca cacaactggt gaaaaagcgc ggtgggattg tcgtgataag
     6601 cgccttcgca ggttagcagt tcgagagtcg gtttttgtgt ttgcattgtt attctctaat
     6661 tttgttcaaa aaaaagcccg ctcattaggc gggctgggta tctgattgct ttacgcatgg
     6721 tgaatacact gcccgtttca ggaagtgcgc caccaacctt tcagtacgaa aattgctttc
     6781 attgtcgata ccctttttac gtgaacttgc gtactagtta actagttcga cgattaattg
     6841 tcaacagctc atttcagaat atttgccaga accgttatga tgtcggcgca aaaaacatta
     6901 tccagaatgg gagtgcgcct tgagcgacac gaattacgca gtgatttacg acctgcacag
     6961 ccataccaca gcttccgatg gctgcctgac gccagaagca ttggtgcacc gtgcagtcga
     7021 gatgcgcgtc ggcaccctgg cgatcaccga ccatgacacc acagctgcaa tcgcgccagc
     7081 aagagaggaa atttcacgtt cgggactggc actgaatctt attcccggcg tggaaatatc
     7141 cacggtctgg gaaaatcatg aaattcatat tgtcgggctg aatattgata ttattcatcc
     7201 gctaatgtgt gagttcctcg cgcagcagac agaacggcgc aatcagcggg cgcagcaaat
     7261 tgccgaaaga cttgaaaaag cgcaaatacc tggggcgctg gaaggcgcac aacgactggc
     7321 gcagggcggc gcagtgacgc gcggtcattt tgcgcgtttc cttgttgagt gcggcaaagc
     7381 cagttcaatg gcggatgtct ttaaaaagta tctggcgcgc gggaaaaccg gatacgttcc
     7441 gccacagtgg tgtacaatag aacaagctat tgatgtcatt catcattctg gcggtaaggc
     7501 ggtgctagct catcctgggc ggtacaatct tagtgctaaa tggctgaaaa gattggttgc
     7561 gcattttgcc gaacacgacg gcgacgcgat ggaagtcgcg cagtgccagc aatcgcccaa
     7621 tgaacgtacc cagctggcga cgcttgcccg ccagcaccat ttatgggcat cacaaggatc
     7681 tgattttcat cagccatgcc cgtggatcga actgggtcgt aaactctggc tgcccgcagg
     7741 cgttgaaggc gtctggcagc tatgggaaca gccgcagaac accacagaga gggaattatg
     7801 agccagtttt tttatattca tcctgataac ccgcagcaac gtctgatcaa ccaggcggtg
     7861 gagatcgtgc gtaaaggcgg ggtgattgtt tatccaaccg attccggcta tgcgctcggc
     7921 tgtaaaattg aagataaaaa cgcgatggag cgtatttgtc gtattcgcca gctgccggac
     7981 ggtcacaact ttaccctgat gtgtcgcgat ctttctgaac tctcgaccta ttcatttgtc
     8041 gataacgttg cgtttcgttt aatgaaaaac aacacgccgg gcaactatac cttcatcctg
     8101 aaggggacga aggaggtgcc acgccgtctg ttgcaggaaa aacgcaaaac catcggtatg
     8161 cgtgtgcctt ctaacccgat tgcgcaggcg ttgcttgaag cactgggcga accgatgctt
     8221 tccacttcgc taatgctgcc aggcagcgaa tttaccgaat ccgatcccga agaaattaaa
     8281 gaccgtctgg aaaaacaggt agatttgatt attcacggcg gctatctcgg gcagaaaccg
     8341 acaacggtta ttgatcttac cgatgatacg ccggtcgtgg tgcgtgaagg cgtaggtgat
     8401 gtgaagcctt tcttataaca aattgcgttc cacggatgga agactatggc agggaagttt
     8461 cgctgcattt tgctattgat agttgggctt tttttctctt cgttaagtta tgcgaaaaac
     8521 acggagatgc cttcttatga agaagggatc tcgctctttg atgttgaagc cactctgcaa
     8581 ccggatgggg tgctcgacat caaagagaac attcattttc aggcgcgaaa tcagcagatt
     8641 aagcacggat tttatcgtga tttaccacga ctatggatgc agcctgatgg ggacgctgca
     8701 ctgctgaact atcatattgt tggcgtcacc cgtgatggta ttcctgaacc ctggcatctt
     8761 gactggcata tcgggttaat gagtattgtc gtgggcgata aacaacgttt cttgcctcaa
     8821 ggcgactatc attatcaaat tcattatcag gttaaaaatg ctttcctgcg tgagggggat
     8881 tctgatctgc taatttggaa cgtgaccggt aaccactggc cgtttgaaat ttataagacc
     8941 cgtttttctc tccagttctc taatattgcg ggtaatccat ttagcgaaat cgatctcttt
     9001 accggagaag agggcgacac atatcgtaat ggccgcatcc ttgaggacgg aagaattgaa
     9061 tcccgcgatc cgttttatcg tgaagatttc acggtactct accgttggcc tcactcgtta
     9121 cttagcaatg ccccggctcc acaaacgacg aatattttca gccatcttct tttaccctcc
     9181 acgtcatcgt tgttaatttg gtttccgtgt ctattcctgg tttgtggatg gttatatctc
     9241 tggaagcgca ggccgcaatt tacgccggta gatgtgattg aaaccgatgt cattccgcca
     9301 gattacacgc tcggcatgtt acgtctcgat gcgaagctgg tttacgacga taaaggtttt
     9361 tgtgccgata tcgtaaatct gatagtgaaa ggaaaaattc atctggaaga tcagtctgac
     9421 aagaaccagc aaaccctgat tcgtgttaat gaaggcgtga ccagaaataa tgcggtatta
     9481 ctgcccgcag agcagttatt actggaagcg ttatttcgta aaggcgataa ggtcgttctt
     9541 acggggagac gcaacagagt cttacgcagg gcatttttac ggatgcagaa attttatctg
     9601 ccgcgtaaaa agtcttcgtt ttaccggcct gatacgtttt tgcaatgggg tggactggca
     9661 atattggcgg tcattctcta cggtaacctg agtcctgtag gctgggcagg aatgagtctg
     9721 gtgggcgata tgtttattat gatctgctgg cttcttcctt ttttattttg ttcccttgag
     9781 cttttgttta cccgcgatga tgacaagcct tgcgttaatc gtgtaatcat cactttgttt
     9841 ttaccgctga tttgttcagg cgtggccttt tattctctct atatcaatgt cggagatgta
     9901 ttcttttact ggtatatgcc agcggggtat tttagcgctg ttttcctgac cggttatctc
     9961 actggtatgg ggtatatttt tctgccaaag tttacccaaa ctgggcagca acgttatgcc
    10021 cacggtgaag ctatcgttaa ctatcttgcg cgtaaagagg cggcaacaca cagtgggcgg
    10081 cggcggaaag gggaaacacg gaaactggat tacgcgttgc taggttgggc agtctcagca
    10141 aaccttggaa gagaatgggt tgcacgtatc accccatcac tcacagcggc tgttagcgcc
    10201 ccggaaattg cccgtagtgg cgttttgttc tcattacaga tgcacctgag tctgggggcc
    10261 aataccagtt tgttggggcg aagttattcc gg
//
LOCUS       DAZKFU020000170        10245 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZKFU020000170 DAZKFU020000000
VERSION     DAZKFU020000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.171"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(200..1600)
                     /gene="asnS"
                     /locus_tag="ACHOQQ_RS17210"
                     /old_locus_tag="pgaptmp_003442"
     CDS             complement(200..1600)
                     /gene="asnS"
                     /locus_tag="ACHOQQ_RS17210"
                     /old_locus_tag="pgaptmp_003442"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGU4471653.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1768..2970)
                     /gene="pncB"
                     /locus_tag="ACHOQQ_RS17215"
                     /old_locus_tag="pgaptmp_003443"
     CDS             complement(1768..2970)
                     /gene="pncB"
                     /locus_tag="ACHOQQ_RS17215"
                     /old_locus_tag="pgaptmp_003443"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGU4471654.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLRNFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3236..5848
                     /gene="pepN"
                     /locus_tag="ACHOQQ_RS17220"
                     /old_locus_tag="pgaptmp_003444"
     CDS             3236..5848
                     /gene="pepN"
                     /locus_tag="ACHOQQ_RS17220"
                     /old_locus_tag="pgaptmp_003444"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGU4471655.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(5891..6658)
                     /gene="ssuB"
                     /locus_tag="ACHOQQ_RS17225"
                     /old_locus_tag="pgaptmp_003445"
     CDS             complement(5891..6658)
                     /gene="ssuB"
                     /locus_tag="ACHOQQ_RS17225"
                     /old_locus_tag="pgaptmp_003445"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4471656.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(6655..7446)
                     /gene="ssuC"
                     /locus_tag="ACHOQQ_RS17230"
                     /old_locus_tag="pgaptmp_003446"
     CDS             complement(6655..7446)
                     /gene="ssuC"
                     /locus_tag="ACHOQQ_RS17230"
                     /old_locus_tag="pgaptmp_003446"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGU4471657.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7458..8603)
                     /gene="ssuD"
                     /locus_tag="ACHOQQ_RS17235"
                     /old_locus_tag="pgaptmp_003447"
     CDS             complement(7458..8603)
                     /gene="ssuD"
                     /locus_tag="ACHOQQ_RS17235"
                     /old_locus_tag="pgaptmp_003447"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGU4471658.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQDLAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(8600..9559)
                     /gene="ssuA"
                     /locus_tag="ACHOQQ_RS17240"
                     /old_locus_tag="pgaptmp_003448"
     CDS             complement(8600..9559)
                     /gene="ssuA"
                     /locus_tag="ACHOQQ_RS17240"
                     /old_locus_tag="pgaptmp_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGU4471659.1"
                     /translation="MRKIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLATRPY
                     AVKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9552..10127)
                     /gene="ssuE"
                     /locus_tag="ACHOQQ_RS17245"
                     /old_locus_tag="pgaptmp_003449"
     CDS             complement(9552..10127)
                     /gene="ssuE"
                     /locus_tag="ACHOQQ_RS17245"
                     /old_locus_tag="pgaptmp_003449"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGU4471660.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
BASE COUNT         2544 a         2775 c         2634 g         2292 t
ORIGIN      
        1 tgtgctatca gctaagaaaa aactaatccg caataagaat gcggattatt ttttgcgcat
       61 aaatgttgca atgtaatatt ccgtaacagg atgatcgtta tggcggggtt gcaaatagat
      121 tgcttgactt aacaaacaga taacttgaca gaaaagataa aaaaagagcc agcgggcgct
      181 ggctcttgaa gacgctaaat tagaagctgg cgttacgcgg agtacgtggg aacggaatca
      241 catcacgcac gttttgcaca ccagttacgt aagcaatcag gcgttcaaaa ccaagaccga
      301 aacctgaatg cggaacagta ccgtagcgac gcagatcgcg ataccaccag taatcttctt
      361 tattcaggcc catttccagc atacgctcgt ccagcacgtc cagacgttct tcacgctggg
      421 agccaccaat gatctcaccg atgcccggag ccagaacgtc catagccgca acggttttac
      481 cgtcttcgtt aagacgcata tagaacgctt taatatcttt cggatagttt ttaaccacta
      541 ccggtgcttt aaagtgttct tccgccagat aacgttcatg ctcagaagag agatcaacgc
      601 cccagtaaac cgggttttca aacttcctgc cgcagttttc gagaatggtc actgcgtcgg
      661 tgtaatccac ctgcgcaaaa tcggcttcaa tgaagcgttc cagacgtgaa acggcatctt
      721 tatctacgcg ttcagcgaag aatttcatgt cgtcagcgcg ttcttcgaga accgctttga
      781 agacatattt cagcatggct tcagccagtc ccgcgatatc gttcaggtta gcaaacgcca
      841 cttccggttc cagcatccag aattccgcca ggtgacggct ggtgttggag ttttcagcac
      901 ggaaagtcgg gccgaaggta taaattttgg acaatgcgca agcgtaggtt tcgccgttca
      961 actggccaga tacggtcagg aaagactctt taccaaagaa gtctttgtcg aaatccactt
     1021 tgccctgatc gttacgcggc aggttttcca gatccagcgt agaaacgcgg aacatttcgc
     1081 cagcaccttc ggtatcagat gcggtaatca gtggcgttga aacccagaag aatccctgct
     1141 cgttaaagaa gcgatgcagc gcctgcgcca gcgtatgacg aacgcgcgcg acggcaccaa
     1201 tcaggtttgt gcgcggacgc aggtgagcga cttcacgcag atactcaatg ctgtggcgtt
     1261 ttgccgccat cgggtacgtg tctggatctt caacccaacc agcaacttca accttgctgg
     1321 cctgaatttc aaattgttgc ccctggcccg gcgacgccac gactttaccc gtcacaatga
     1381 ccgagcaacc ggtggtcaga cgcaggacgt cttcattgta attgggcaga gaattattga
     1441 tgacagcctg tacaggatca aagcaggaac cgtcataaac ggcgaggaag gagatgccag
     1501 cttttgaatc tcggcgggta cgtacccatc cgcgtacggt gacttcgctg tcaacggcta
     1561 cgcggccctg gagtacgtcg gctacaggca caacgctcat aatattctct ctgttaatag
     1621 tcggaaaaaa taaacacttg tccacccgta aatgggggga tacctatgtt acctggcatc
     1681 tgcaatcaga caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa
     1741 agggagccga ttagctccct gatgatatta actggctttt ttaatatgcg gaaggtcgaa
     1801 cgctttgcgc agcgcccgaa caaacgcttt atcgtggcag atagttttgc cagggctgtc
     1861 agaaagtttc gccaccggtt taccgttaca ctctaccaac ttaatcacaa tattcagggg
     1921 ttttacctgg gggatatcgc aggtcaggcg agtcccaata ccaaaactta attgcacgcg
     1981 ggaagagaag tggcggtata gctcaaccgc tttgcgtaaa tccagattgt cagagaaaac
     2041 cagcgtttta ctctgtggat caattcccag cttttcataa tgtgcaatgg ctttttcacc
     2101 ccattcaacc gggtcgccag agtcatgacg caggccctga taccgactag cgaactcgac
     2161 accgaaatca cgcaggaaag catccatagt gatgcagtcg gttaatgcaa tgccaagttg
     2221 gtcgggatac tcttccagcc aggcagcaag tgcagctcgc tggctgttgg ctagatccgg
     2281 gctgatttgc tgatgtgcct ggaaccattc gtgtgcctgt gttcccatcg gcgtgaggga
     2341 aagccgacgc gccagatcgt agttgctggt gcccacgaac caagattcct gttgcagacg
     2401 cttaacgatg gtttcttgta cttcgcgaga aaaacggcga cgggtgccaa aatccatcag
     2461 atggaagcgc gacatatcaa gaccggcggt taacgccgag aagtcgacta atttgctttc
     2521 cagcgtgtcg agggcttgcg caacgtcggc ctgcggtgag cgatagcgat gtaccatttc
     2581 actgataacc gccagcaaag gaacttccca gaggatgact tcacgccacg ggccgcttaa
     2641 acgaatatcc agcttgccat tatcgttgga cacggtgact tgttccgggt taaagcggaa
     2701 attacgcagc cagttgagat agtcggcctt aaagaaaggc agcgcagaga gccattgata
     2761 ttcatcatcc tgcaggcgca ggtgctgcat cgcctgaacc tgttcacgaa tagcatcggc
     2821 ataaataccc agcagatcgt cacctcggca acgaaactcc gccgcgacat gcacatcgta
     2881 atagtgatga aacacggctt gctgcatatg caacttataa gcatctgtat ccagcaacga
     2941 gtgcagaaca ggagaagcga attgtgtcat aggtgcgcag tagcgtcctc gtacaggagc
     3001 gtttagtaca ataaacatct tcaggaactg ctggagtata ccttgtttcg caatttattg
     3061 aaccccgatc acaccatatg ccacctttct ggtcgatggc attacgcggt gtatgttata
     3121 aaaatgtagc aataaaggcg tttgtacctg aaaagatgaa gattctgcat agcgcgattt
     3181 acgcaacagg aatagactga acaccagact ctataaaaga tgctaaaggt tatttatgac
     3241 tcaacagcca caagccaaat accgtcacga ttatcgtgcg ccggattacc agattactga
     3301 tattgacttg acctttgacc tcgacgcgca aaagacggtc gttaccgcgg tcagccaggc
     3361 tgtccgtcat ggtgcatcag atgctcccct tcgtctcaac ggcgaagacc tcaaactggt
     3421 ttctgttcat attaatgatg agccgtggac cgcctggaaa gaagaagagg gcgcactggt
     3481 catcagtaat ttgccggagc gttttacgct taagatcatt aatgaaataa gcccggcggc
     3541 gaatacggcg ctggaagggc tttatcagtc aggcgatgcg ctttgcaccc agtgtgaagc
     3601 cgaaggtttc cgccatatta cgtattatct cgaccgcccg gacgtgctgg cgcgttttac
     3661 caccaaaatt attgccgata aaatcaaata tcccttcctg ctttccaatg gtaaccgcgt
     3721 tgcgcaaggc gaactggaaa acggacgcca ttgggtacag tggcaggacc cgttcccgaa
     3781 accgtgctac ctgtttgcgc tggtggcagg cgactttgat gtactgcgcg atacctttac
     3841 cacgcgttct ggtcgcgaag tagcactgga gctgtacgtc gatcgcggca accttgatcg
     3901 cgcgccgtgg gcgatgacct cgctgaaaaa ctccatgaaa tgggatgaag aacgctttgg
     3961 cctggagtat gacctcgaca tctatatgat cgtcgcggtg gatttcttca atatgggcgc
     4021 aatggagaat aaggggctga atatctttaa ctccaaatat gtgctggccc gcaccgatac
     4081 cgccaccgac aaagattacc tcgatattga acgcgttatc ggccatgaat atttccataa
     4141 ctggaccggt aaccgagtga cctgccgcga ctggttccag ctcagcctga aagaaggttt
     4201 aaccgtcttc cgcgatcagg aattcagctc tgacctcggt tcccgcgcgg taaaccgtat
     4261 caataatgtg cgcaccatgc gcggattgca gtttgcggaa gacgccagcc cgatggcgca
     4321 cccaatccgc ccggatatgg tcattgagat gaacaacttc tacaccctga ccgtttacga
     4381 gaagggcgcg gaggtgattc gcatgatcca caccctgctt ggcgaagaaa atttccagaa
     4441 agggatgcag ctctatttcg agcgtcatga tggtagcgca gcgacctgtg acgattttgt
     4501 gcaggcgatg gaagatgcgt cgaatgtcga tctctcccat ttccgccgtt ggtacagcca
     4561 gtccggtacg ccggttgtga ccgtcaaaga cgactacaat ccggaaaccg agcagtacac
     4621 cctgaccatc agccagcgca cgccagccac gccggatcag gcagaaaaac agccgctgca
     4681 tattccgttt gccatcgaac tgtatgataa cgaaggcaaa gtgatcccgt tgcagaaagg
     4741 cggtcatccg gtgaattccg tgctgaacgt cacccaggcg gaacagacct ttgtttttga
     4801 taatgtctac ttccagccgg tgcctgcgct gctgtgcgaa ttctctgcgc cagtgaaact
     4861 ggaatataag tggagcgatc agcaactgac cttcctgatg cgtcatgcgc gtaatgattt
     4921 ctcccgttgg gatgcggcgc aaagtttgct ggcaacctac atcaagctga acgtcgcccg
     4981 tcatcagcaa gggcagccgc tgtctctgcc ggtgcacgtt gctgatgctt tccgcgcagt
     5041 gttgctcgat gaaaaaatcg atccggcgct ggcggcagaa attctgacgc tgccttcggt
     5101 aaatgaaatg gcggaactgt tcgatatcat cgacccgatt gccattgccg aagtccgtga
     5161 agcactcact cgtactctgg cgacagaact ggcagatgag ctgctggcta tttacaacgc
     5221 gaattaccag agcgagtacc gcgttgagca tgaagatatt gcgaaacgca ctctgcgtaa
     5281 tgcctgcctg cgtttcctcg cttttggtga aacgcatctg gctgatgtac tggtgagcaa
     5341 gcagtaccac gaagcgaaca atatgactga tgcgttggcg gcgctttctg cggcggttgc
     5401 tgcacagctg ccatgccgtg acgcgctgat gcaggagtac gacgacaagt ggcatcagga
     5461 cggtctggtg atggataaat ggtttatcct gcaagccacc agcccggcgg cgaatgtgct
     5521 ggagacggtg cgcggcctgt tgcagcatcg ctcatttacc atgagcaacc cgaaccgtat
     5581 tcgttcgttg attggtgcgt ttgcgggcag caacccggca gcgttccatg ccgaagatgg
     5641 gagcggttac cagttcctgg tggaaatgct taccgacctc aacagccgta acccgcaggt
     5701 ggcttcgcgc ctgattgagc cgctgattcg cctgaaacgt tatgatgcca aacgtcagga
     5761 gaaaatgcgc gcggcgctgg aacagttgaa agggctggaa aatctctctg gcgatctgta
     5821 cgagaagatc accaaagcac tggcttaata aataaccgaa tggcggcaat agcgccgcca
     5881 ttcggggatt ttacccctgt tttctcaggc gagtttcaga ctcaccacgc tgcattaccc
     5941 gttgcaacac ttccgcttcc agctccgcta atcgcaccga ccctaaccgg cgcggacggg
     6001 ggatatccac cgtcaaatcc agaccaattt ttccctcttc aattaacaac acccggtcag
     6061 ccattgctac cgcttcgctc acatcatgcg tcaccaacag tacggtaaag ccgtgctcct
     6121 gccaaagtga cacaatcaaa tcctgcatct cgagtcgcgt taaggcatcc agcgccccga
     6181 gtggttcatc aagcaacaat aaacccggtc gatgaatcaa cgctcttgcc agcgccactc
     6241 gctgtttctg cccgccagaa agtgcggcag gccattcccc ggcgcgattc tccagtccta
     6301 ccgcagccaa cgcccgacgc gcggcatcgc gccactggcc tttaagacct aacccaacgt
     6361 tatcaatcac cgatttccac ggcagcagac gcgcatcctg aaacatcatt cgcgtgtctt
     6421 cctgaatttc agccagcggt gtggtgcccg ctaacacatc gccagcggtt ggcgtttcca
     6481 gacctgccag caggcgcagc agggtacttt tgccaccacc gctgcggccc accaccgcca
     6541 caaactgacc tgccggaata tgtaaatcca gttggttcag gacgatattt tctgcgtaat
     6601 gtttgcttac tgcattgagc aacaatggtg tgccctggtt cagacgagca gtattcatac
     6661 cgtggcctcc ttcaaatgat aagccgggtt ccagcgcaac cagaggcgct ctaacagctg
     6721 cgcgctgacg tcagccagtt tgccgagcag ggcgtaaaga ataatggcga ccaccaccac
     6781 gtccgtttgc aaaaactccc gcgcattcat cgccagataa ccaatgcctg aattggcaga
     6841 aatggtttcg gcaacaatca gcgtcagcca catcaggccc aacgcaaaac gcacgccgac
     6901 cataattgag ggcagggcac caggcaggat cacatggata aacagcggta tgccggataa
     6961 tccatagcta cgcgccatct ccaccaaccc gcgatcgata ttacggatgc catgccaggt
     7021 gttgatataa atggggaata gcgtaccgag cgccaccaga aagatttttg cggattcatc
     7081 aatgccaaac cacaaaatca ccagcgggat cagcgccaga tgcggcacgt tgcgcaacat
     7141 ctgaattgag gtatccagca gccgttctcc ccagcgagat agcccgctaa tcagtcccaa
     7201 aatcaacccc agcgatccgc caattgaaaa gccaatcagc gcccgccagg agctgatcgc
     7261 cagatgctgc cacagttcgc cgctggcgga gagcgtccag aacgccgtca ccactccttc
     7321 cggtgaaggc aaaatacgcg tcgacagcca gccaaccgag gaggccagtt gccacaccgc
     7381 cacgatgccc accggtaaaa accagggggc aacgcgcaat aaccacttct tcactggcgt
     7441 tgccatcatc gtgttcctta gctttgcgcg actttacggg ggataaaatc attcgccacc
     7501 gcttcgcctt gcggattcag cggctgcggc tggggaattt ccggaatggc gacatccaga
     7561 tgtgggaaca gcaactcgcc gacccgatac gcttcttcca gatgcggata gcccgaaagc
     7621 acaaagctgt cgatgccaag cgcggcatat tcgttgattc gcgcagcgac cgtaggacca
     7681 tcgcccacca gcgccgtccc ggcaccgccg cgcactaagc caacgcccgc ccataaattg
     7741 gggctgatct ccagattgtc gcgcttgcca ttatgcaacg ccgccattcg ctgttgcccc
     7801 acggaatccg tgcgggcaaa cgcggcctgt gctttggcga tagtctcatc atcaagatgc
     7861 gaaattaacc gttcggcggc ctgccacgct tcgtcgttgg tttcgcgaac aatcacatgc
     7921 agacgaatac cgaaacgaat tttgcgtcca tgcgcggccg ctttcgcccg cacttgttcg
     7981 attttctctt ttaccagctc tggcggttcg ccccaggtga gataaagatc aacttgttcg
     8041 gcggccagat cctgagcgac gtctgaagat ccgccaaagt aaagcggggg ataaggctgt
     8101 tgaatcggcg gaaagagcag tttggctcca cgcacatgaa tatgtttgcc gttgaaatcg
     8161 acggtttcgc ccagcaataa acgccgccag acctgggtaa attccgccga ggcttcgtag
     8221 cgctcgctat gatcaaggaa cacgccatcg cctgccagct cttgtggatc gctgcctgtg
     8281 accaggttaa ataacgcacg tccgttagag agacggtcaa gcgtggcggc ctggcgggcg
     8341 gcaacggtag gagacgtgac actgggacgc agagcgacaa gaaacttcag ccgctgcgtc
     8401 accgggatca tcgacgctgc aaccagccac gcatcttcgc aggagcgccc cgttgggatc
     8461 agcacaccgg tatagcccag acgatccgcc gcttgcgcaa tttgttgcag ataaccgtga
     8521 tcaaccgggc gtgaaccttc ttccgttccc agataatgcc cgtcaccgtg ggtcggtaaa
     8581 aaccagaaca tattcagact cataattgtt ttccttccag ttgagtgggc tgccagatgc
     8641 gctggcgaat atcgactttt tttggcacca gacggttttc ataaaacaga tctgccgttt
     8701 gctgttgtaa ggcagcaact tctgcgctca ccggcttgat agtggtaggg ggacgatgat
     8761 ctaaataaga ggcaatcacc ggcgccggta agcccatcgt ttttgccagt aaagcgatac
     8821 tttgctcgcg ctgactgcgg gttaacgcat cggcttcgct aaaggttgcc agtacgccct
     8881 gaataaatgc gccgtttttt actgcatacg gacgggttgc cagataaaac gatccggttt
     8941 gattgaggtc ggtaccgtct ttcagcaccc gcacgccgcc ctgtaataac gcggcggagt
     9001 agtagggatc ccagatagcc caggcatcga cgttaccctg ctggaatgcg gcgcgggcat
     9061 cagctggcgt cagataagtg ggctggatgt cagtaaactt aagtccggcc tggcgtagtg
     9121 cgcgcagtaa aagattgtgt gaactggaac ctttctggaa cgcaactttg tggcctttaa
     9181 gatcggctac ggttttgatc gggctgtttt ctgccaccag aatcacctcg gcttttggct
     9241 tcggcggctc gacgcccaca tataccaaat cagccccggc agcctgggca aagattggcg
     9301 gaatatcccc ggtactgccg agatcaatac tgccaacgtt taacgcttct aacatttgcg
     9361 gacccgcggg gaactccacc caggagattt ttgattgcgg atagcgtttt tccagtaact
     9421 ggtggctttt tgccaatacc ataccaatac tgcctttctg atagcctata cgtaacgctt
     9481 caggcgagga ttctgcagca accacaatcg tagagacgct aagcaatccc accagcgcca
     9541 gtttaatgat cttacgcatg ggcattacct cgcagagaat gaagatcagg aacctgaaca
     9601 tcgcggcggt gcaatgcctg ccagaaagtt tctagcgcgg tgtcaagacg ggtttgtaaa
     9661 ttcggcgtga actggggttt gtgatggtaa tcaattactt gtgagtcatc ggcaaacacg
     9721 ccgtgcagga tctcctgggc tttcagcgca cttaaaactg gcttaagggc ataatcgacc
     9781 gccagcagat gggccaccgt accgcccgtc gccagcggta gcaccacttt gccttgcaac
     9841 gcacgttctg gcagcaggtc gagcagggtt ttcagcgcac cggaataagc ggctttatac
     9901 acaggcgtgg cgacaatcag cccgtcagcc tgttgcaact gttcggtgaa ggtatggagt
     9961 gccggactat cgaaacgagc ataaagcaga tcttccgcgg cgaagttttg cagattccag
    10021 tgataaacct caacatccag gccatttagt ttttcccgcg catattccag caaggagctg
    10081 gagcgagaag gaaagcgagg actacccgcc agggtgatga cacgcatact ctctccttat
    10141 aaccaattgt tcttgttttg ttaacattga taacaatttg ctcagtctgt cggagagaca
    10201 agaaaatgcc aaatataaat tttgtgtatc tttttctgaa aataa
//
LOCUS       DAZKFU020000171        10224 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZKFU020000171 DAZKFU020000000
VERSION     DAZKFU020000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.172"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..727
                     /gene="yahL"
                     /locus_tag="ACHOQQ_RS17250"
                     /old_locus_tag="pgaptmp_003450"
     CDS             <1..727
                     /gene="yahL"
                     /locus_tag="ACHOQQ_RS17250"
                     /old_locus_tag="pgaptmp_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HGU4471661.1"
                     /translation="DIKSSLVSCVDIFKVLIKQYYDYPYDCRDDLVDDDKLIHLMAAV
                     RDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVFRILENQKGTVWVYDQDDAFL
                     DIKANVQAGRFTGLKKLVQFIDSVRTDCKCILLEHHMPLLRLFPKGKECMHVEKWLRE
                     MSSIPETDAPIKQVLAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRDDARLNEWIS
                     NRVQELGDRYYPVNKHVKIRYTL"
     gene            1142..1387
                     /gene="yahM"
                     /locus_tag="ACHOQQ_RS17255"
                     /old_locus_tag="pgaptmp_003451"
     CDS             1142..1387
                     /gene="yahM"
                     /locus_tag="ACHOQQ_RS17255"
                     /old_locus_tag="pgaptmp_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HGU4471662.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAWIRQIRLIT"
     gene            complement(1404..2075)
                     /locus_tag="ACHOQQ_RS17260"
                     /old_locus_tag="pgaptmp_003452"
     CDS             complement(1404..2075)
                     /locus_tag="ACHOQQ_RS17260"
                     /old_locus_tag="pgaptmp_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGU4471663.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            2222..2497
                     /gene="yahO"
                     /locus_tag="ACHOQQ_RS17265"
                     /old_locus_tag="pgaptmp_003453"
     CDS             2222..2497
                     /gene="yahO"
                     /locus_tag="ACHOQQ_RS17265"
                     /old_locus_tag="pgaptmp_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HGU4471664.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(2598..4184)
                     /gene="prpR"
                     /locus_tag="ACHOQQ_RS17270"
                     /old_locus_tag="pgaptmp_003454"
     CDS             complement(2598..4184)
                     /gene="prpR"
                     /locus_tag="ACHOQQ_RS17270"
                     /old_locus_tag="pgaptmp_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGU4471665.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLRYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            4423..5313
                     /gene="prpB"
                     /locus_tag="ACHOQQ_RS17275"
                     /old_locus_tag="pgaptmp_003455"
     CDS             4423..5313
                     /gene="prpB"
                     /locus_tag="ACHOQQ_RS17275"
                     /old_locus_tag="pgaptmp_003455"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGU4471666.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARNQVK"
     gene            5473..6642
                     /gene="prpC"
                     /locus_tag="ACHOQQ_RS17280"
                     /old_locus_tag="pgaptmp_003456"
     CDS             5473..6642
                     /gene="prpC"
                     /locus_tag="ACHOQQ_RS17280"
                     /old_locus_tag="pgaptmp_003456"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGU4471667.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSAIGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            6676..8127
                     /gene="prpD"
                     /locus_tag="ACHOQQ_RS17285"
                     /old_locus_tag="pgaptmp_003457"
     CDS             6676..8127
                     /gene="prpD"
                     /locus_tag="ACHOQQ_RS17285"
                     /old_locus_tag="pgaptmp_003457"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGU4471668.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPTFT
                     ADYHDPEKRAIANAVTLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            8167..10053
                     /gene="prpE"
                     /locus_tag="ACHOQQ_RS17290"
                     /old_locus_tag="pgaptmp_003458"
     CDS             8167..10053
                     /gene="prpE"
                     /locus_tag="ACHOQQ_RS17290"
                     /old_locus_tag="pgaptmp_003458"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HGU4471669.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         2493 a         2626 c         2753 g         2352 t
ORIGIN      
        1 ggatatcaaa agctccctgg tttcctgtgt cgatatattc aaggttttga ttaaacaata
       61 ttatgattat ccttatgatt gtcgtgatga tttagtcgat gacgacaaac tcattcatct
      121 catggctgcc gtacgagact gtgagtggtc agacgataac gcactcacca taaatgtgca
      181 gtttaatgat ttccctggat tttatgactg gatggattac cctgatcacc cggttaagtt
      241 tgtttttcgc atacttgaaa atcaaaaagg gacagtttgg gtatatgacc aggatgatgc
      301 gtttcttgat attaaagcaa acgtgcaggc tggacgcttt accgggctta agaaattagt
      361 gcagtttatc gattcagtgc gaacagattg caaatgtatt cttcttgaac accatatgcc
      421 tttgctgaga cttttcccta aagggaaaga gtgtatgcat gtggaaaaat ggcttcgcga
      481 aatgtcgtct attccggaga cagacgcgcc cattaagcag gtgcttgcgc atggattatt
      541 attacactta aaaaatatct accctgtttt tcctgaatca ctggtgatgt tattgctctc
      601 tgttctggac gtaaaaacat acagagatga cgcacgatta aatgaatgga tatctaatag
      661 agtgcaggaa ttaggtgaca gatattatcc tgtaaataag cacgttaaaa tccgctacac
      721 tctttagttc agaaagggtt ccacctgcaa gttgttattc cacctgagag tgaatcgcaa
      781 tgcaggtaat catttcatct gtatatctgt atgccaactg gcattccatc tcttttttcg
      841 ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagtag tggtttcgca
      901 gccatgcggt gtataaaaaa aatgatctca tgcagatgtt ttgtgaatgt gtgggttgac
      961 attcatatga aaaaaatcat aattccatca tgtttgtgtg gggtcttttc tgtatcttac
     1021 gcatcgcact caagcctgac agaaaatatg ctgtaaggct catatcaaaa accgccatta
     1081 gctcatcagg aagagcagac gacaaccagg attgttgtat ggtacggggt tcgaggcctc
     1141 gatggcggtc caacttttca aaactcttct gaatcagatc cccttattat catccctgca
     1201 aagtggaaca ttgccattgt ttggctattc aggacgggtc agaccaatga aaaaagccca
     1261 cacaggggag agtgggctga aatgggaagc taaagactca agtaaactta tcggaaataa
     1321 ggaccacgca ttacgggggc tatcatcgcc gatggcctgg attcggcaaa tcaggttgat
     1381 tacgtagaga gtaaattatc tgctcaccgc tgcgtcaccc cttcgtaaat caggcgtagc
     1441 gcgaatacac caataattgc accaataacc cgactggcaa cgcgttgcat acgcccataa
     1501 gcacgacgca cagcgggcaa agaaaacgcc tgactaagaa agactcgcca gataactgat
     1561 gcgagcacaa tccccgccca ggccattaaa cgtgcccatg ttggtgtttc ggcatttaat
     1621 gttactgaga aaatactgat aaaaaataaa acggtttgcg ggttagagag atcggtaatt
     1681 aatccgcggc gaaaaaagac ataccagggg gcgctaatcg gttgttgtag tgtgctcatt
     1741 tgcggtgttg actggcggcg catgctgcac cacgcaaacc ataagagata agcgccgccg
     1801 acgattctga taagcgaaaa aatctcctca cactgcgtaa ttagtgttgc aagaccaaac
     1861 aaacccaacc cggaataaaa tgcatcgccc agcgccacgc ccagcccggt cagcacccct
     1921 gcgcgtcgac cggaagccag gctggtttgt actaccacaa agagattggc tcccggatta
     1981 aaaaaagtaa tcacgaacag tcccacggtc aggtaaacgg catgcaaagg atccatagtg
     2041 atttcatcca taaataagtg aactaactgc atcatatttc taccaaaaat aatcgggtgc
     2101 gagagagatc acaaagtgtc ttatttccgg ttactggcgt ttatgccctg actgaactaa
     2161 ttattaatca acccaataat gtgggtgggt gatagtgtga taacaactct ggagccgtaa
     2221 tatgaaaata atctctaaaa tgttagtcgg tgcgttagcg tttgccgtta ccaatgtcta
     2281 tgccgctgaa ttgatgacca aagcggaatt tgaaaaagtt gaatcgcagt atgaaaaaat
     2341 aggtgatatt tcaaccagta atgaaatgtc gactgcagat gcaaaagaag atttaatcaa
     2401 aaaagccgat gaaaaagggg ctgatgtgtt ggtgctgacc tctggtcaaa ctgacaataa
     2461 gatccacggc acggcggata tttataagaa gaagtaattc tgaaccctat gtaaacatcc
     2521 ccgatgcgta agtttatcgg tgatcatcat ctattgaaat ttatgccgga taaagcgttc
     2581 gcgctgcatt cggcagttca gcttttcagc cgccgccaga acgtcgtccg actgatgcct
     2641 aaataattcg ccgctgctgt tttatcgcca ttaaatttct ccagtgcctg ttgtggtgtc
     2701 agtaagcgtg gagcgggagt tttcgccgac tcgcgcgcca gttccggcag tagcagctgc
     2761 aaaaattgcg gcgttaaatc cggcgtcggt tccacactta aaaatagcgc cagtcgctcc
     2821 atcatattgc gcagttcacg aatattgccc ggccagtcgt agcgcagcag cacagtttca
     2881 cttgcctgta acccctggcg taatgcagca gaaaacgggg cggagagcgc cgccagagac
     2941 actttcaaaa aactttccgc cagtggcaga atatctgcca cccgctcgcg cagtggtggc
     3001 aattgcagac gcaaaatact caaccgataa aacagatcac ggcgaaactg cccttgccgc
     3061 atatcttctt ccagattgca gtgagtggcg ctaatgaccc gcacatccac cggaacaggc
     3121 tgatgcccgc cgacgcgagt aacctctttt tcttccagca cccgtaacag gcgagtctgc
     3181 aaaggtagcg gcatttcgcc aatctcatcc aaaaacagcg tgccaccgtg ggcgatttcg
     3241 aacagtccgg cgcgacctcc gcgtcgcgag ccggtaaacg ccccttcctc atagccaaac
     3301 agctccgctt ccagcagcga ttcggcaatc gccccgcagt tgacggcaac aaacggatgc
     3361 gactttttgc cctgtcgcgc atcgtggcgg gcaaaatatt cccgatgaat tgcctgggcc
     3421 gccagctctt tgcccgtccc cgtttccccc tcaatcaaca ccgccgcact ggagcgggca
     3481 tacagcaaaa tagtctgccg tacttgttcc atctgtggtg attgaccgag catatcgccc
     3541 agcacgtaac gagtacgcag ggcgttacgg gtagcatcgt gagtgttatg gcgtaacgac
     3601 atgcgcgtca tatccagcgc atcgctgaac gcctggcgca cggtggcggc ggaatagata
     3661 aaaattccgg tcattccggc ttcttctgcc agatcggtaa tcagccccgc gccgaccacc
     3721 gcttcggtgc cgttagcttt tagctcgtta atctgcccgc gtgcgtcttc ttcggtaatg
     3781 tagctacgtt gatcgaggcg caaattaaag gttttttgaa acgccaccag tgccggaata
     3841 gtttcctgat aagtgacaac gccgatagaa gaggtgagct ttccggcttt tgccagtgct
     3901 tgtaacacat cgtagccgct cggtttaatc aaaataactg gcactgacag gcggcttttc
     3961 aggtacgcgc cgttagagcc agcagcgatg atggcgtcac agcgttcgtt tgccagtttc
     4021 ttgcggatgt aggtcactgc tttttcaaag ccaagctgaa taggggtaat gttcgccagg
     4081 tgatcaaact cgaggctgat atcgcgaaac agctcgaaca ggcgcgttac agataccgtc
     4141 cagataaccg gtttgtcgtc attcagccgt ggtggatgtg ccatagcgca ccgcaaagtt
     4201 aagaaaccga atattgggtt tagccttgtt tcataattgt tgcaatgaaa cgcggtgaaa
     4261 cattgcctga aacgttaact gaaacgcata tttgcggatt agttcatgac tttatctcta
     4321 acaaattgaa attaaagatt taattttatt aaggcaattg tggcacaccc cttgctttgt
     4381 ctttatcaac gcaaataaca agttgataac aaaggatggg ctatgtctct acactctcca
     4441 ggtaaagcgt ttcgcgctgc actgtctaaa gaaaatccat tgcagattgt tggcaccatt
     4501 aacgccaacc atgcgctgtt ggcgcagcgt gccggatatc aggcaattta tctctccggc
     4561 ggtggcgtgg cggcagggtc gctggggctg cccgatctcg gtatttctac ccttgatgat
     4621 gtgctgaccg acattcgccg tatcaccgac gtttgttcgc tgccgctgct ggtggatgcg
     4681 gatatcggtt ttggttcttc ggcctttaac gtggcgcgta cggtgaaatc gatgattaaa
     4741 gccggtgcgg caggattgca tattgaagat caggttggtg cgaaacgctg cggtcatcgt
     4801 ccgaataaag cgatcgtctc gaaagaggag atggtggatc ggatccgcgc ggcggtggat
     4861 gcgaaaaccg atcctgattt tgtgatcatg gcgcgcaccg atgcgctggc ggtagagggg
     4921 ctggatgcgg cgatcgagcg tgcgcaggcc tatgttgaag cgggtgccga gatgttgttc
     4981 ccggaggcga ttaccgaact cgccatgtac cgccagtttg ccgatgcggt gcaggtgcca
     5041 atcctcgcca acattaccga atttggcgcc acgccgctgt ttaccaccga cgaattacgc
     5101 agcgctcatg tcgcgatggc gctgtacccg ctttcagcgt ttcgcgccat gaaccgcgcc
     5161 gccgaacatg tctacaacgt cctgcgccag gaaggcacgc agaaaagcgt catcgacacc
     5221 atgcagaccc gcaacgagct gtacgaaagc atcaactact accagtacga agagaagctc
     5281 gacgacctgt ttgcccgtaa ccaggtgaaa taataaacgc ccgttggttg tattcgacaa
     5341 ccgatgcctg atgcgccgct gacgcgactt atcaggccta cgggtgtact gaactgtagg
     5401 tcggataaga cgcatggcgt cgcatccgac aacaatctcg accctacaaa tgataacaat
     5461 gacgaggaca acatgagcga cacaacgatc ctgcaaaaca gtacccatgt cattaaaccg
     5521 aaaaaatcag tggcgctttc tggcgttccg gcgggcaata cggcgctctg caccgtgggt
     5581 aaaagtggca atgacttgca ttaccgtggt tacgatattc ttgatctggc ggaacattgc
     5641 gaatttgaag aagtggcgca tctgctgatc cacggcaaac tgccgacccg tgacgaactc
     5701 gccgcctaca aaacaaaatt gaaagccctg cgcggtttac cggctaacgt gcgtactgtg
     5761 ctggaagcct taccagcggc gtcgcatccg atggatgtca tgcgcaccgg cgtttctgcg
     5821 atcggctgca cgctgccaga aaaagagggg cacaccgttt ccggtgcgcg ggatattgcc
     5881 gacaaactgc tggcatcact tagctcgatt ctcctttatt ggtatcacta cagccataac
     5941 ggcgaacgca tccagccgga aaccgatgac gactccatcg gcggtcactt cctgcatctg
     6001 ctgcacggcg aaaagccgtc gcaaagctgg gaaaaggcga tgcatatttc gctggtgctg
     6061 tacgccgaac acgagtttaa cgcctccacc tttaccagtc gggtgattgc gggcactggc
     6121 tctgatatgt attccgcgat tattggcgcg attggcgcac tgcgcgggcc gaaacacggc
     6181 ggggcgaatg aagtgtcgct ggagatccag caacgctacg aaacgccaga cgaagccgaa
     6241 gccgatatcc gcaagcgggt ggaaaacaaa gaagtggtca ttggttttgg gcatccggtt
     6301 tataccatcg ccgacccgcg tcatcaggtg attaaacgtg tggccaagca gctctcgcag
     6361 gaaggcggct cgctgaagat gtacaacatc gccgatcgcc tggaaacggt gatgtgggag
     6421 agcaaaaaga tgttccccaa tctcgactgg ttctccgctg tttcctacaa catgatgggc
     6481 gttcccaccg agatgttcac accactgttt gttatcgctc gcgtcaccgg ctgggcggcg
     6541 cacattatcg aacaacgtca ggacaacaaa attatccgtc cttccgccaa ttatgttgga
     6601 ccggaagacc gccagtttgt cgcgctggat aagcgccagt aaacctctac gaataacaat
     6661 aaggaaacgt acccaatgtc agctcaaatc aacaacatcc gcccggaatt tgatcgtgaa
     6721 atcgttgata tcgtcgatta cgtcatgaac tacgaaatca gctctaaagt ggcctacgac
     6781 accgcacatt actgcctgct cgacacgctc ggctgcggtc tggaagctct cgaataccca
     6841 gcctgtaaaa aactgctggg gccaattgtc cccggcaccg tcgtacctaa cggcgtgcgc
     6901 gtccccggaa ctcagttcca gctcgacccc gtccaggcgg catttaacat cggcgcgatg
     6961 atccgttggc tcgatttcaa cgatacctgg ctggcggcgg agtgggggca tccttccgac
     7021 aacctcggcg gcattctggc aacggcggac tggctttcgc gcaacgcggt cgccagcggc
     7081 aaagcgccgt tgaccatgaa acaggtgctg accggaatga ttaaagccca tgaaattcag
     7141 ggctgcatcg cgctggaaaa ctcctttaac cgcgtcggcc tcgaccacgt tctgttagtg
     7201 aaagtggctt ccaccgccgt ggtcgccgaa atgcttggcc tgacccgcga ggaaattctc
     7261 aacgctgttt cgctggcgtg ggtggacggt cagtcgctgc gcacctatcg ccatgcgccg
     7321 aacaccggca cgcgtaaatc ctgggcggcg ggcgatgcca cttcccgcgc ggtacgtctg
     7381 gcactgatgg cgaaaacggg cgaaatgggt tacccgtcag ccctgactgc gccggtatgg
     7441 ggcttctacg acgtctcctt taaaggtgaa tcgttccgct tccagcgtcc gtacggttct
     7501 tacgtcatgg agaatgtgct gttcaaaatc tccttcccgg cggagttcca ctcccagacg
     7561 gcagttgaag cggcgatgac gctctatgaa cagatgcagg cagcaggcaa aacggcggcg
     7621 gatatcgaaa aagtgaccat tcgcacccac gaagcctgta ttcgcatcat cgacaaaaaa
     7681 gggccgctca ataacccggc tgaccgcgac cactgcattc aatacatggt ggcgatcccg
     7741 ctgttgttcg ggcgcttaac ggcggcagat tacgaggaca acgttgcgca agataaacgc
     7801 atcgacgccc tgcgcgagaa gatcaattgc tttgaagatc cgacgtttac cgctgactac
     7861 cacgacccgg aaaaacgcgc catcgccaat gccgttactc ttgagttcac tgacggtacg
     7921 cgctttgaag aagtggtggt ggagtacccc attggtcatg ctcgccgccg tcaggatggc
     7981 attccgaagc tggtagataa attcaaaatc aatctcgcgc gccagttccc gactcgccag
     8041 cagcagcgca ttctggaggt ttctctcgac agagctcgcc tggaacagat gccggtcaat
     8101 gagtatctcg acctgtacgt catttaagta aacggcggta aggcgtaagt tcaacaggag
     8161 agcattatgt cttttagcga attttatcag cgttcgatta acgaaccgga gcagttctgg
     8221 gccgagcagg cccggcgtat tgactggcag acgcccttta cgcaaacgct cgatcacagc
     8281 aatccgccgt ttgcccgttg gttttgtgaa ggccgaacca acttgtgtca caacgctatc
     8341 gaccgctggc tggagaaaca gccagaggcg ctggcattga ttgccgtctc ttcggaaaca
     8401 gaagaagagc gcacctttac ctttcgccag ctgcatgacg aagtgaacgc ggtggcgtca
     8461 atgctgcgct cactgggcgt gcagcgtggc gatcgggtgc tggtgtatat gccgatgatt
     8521 gccgaagcgc atattaccct gctggcctgc gcgcgcattg gcgctattca ctcggtggtg
     8581 tttgggggat tcgcctcgca cagtgtggcg gcgcgaattg atgacgctaa accggtgctg
     8641 attgtctcgg cagatgccgg agcgcgcggt ggcaaaatca ttccctataa aaaattgctc
     8701 gacgatgcga taagtcaggc gcagcatcag ccgcgccatg ttttgctggt agatcgtggg
     8761 ctggcgaaaa tggcgcgcgt tagcgggcgg gatgtcgatt tcgcgtcgtt gcgccatcag
     8821 cacatcggcg cgcgggtgcc ggtggcctgg ctggaatcaa acgaaacctc ctgcattctc
     8881 tacacctccg gcacgaccgg caaacctaaa ggcgtgcagc gtgacgtcgg cggatatgcg
     8941 gtggcgctgg cgacctcgat ggacaccatt tttggcggca aagcgggagg tgtgttcttt
     9001 tgtgcttcgg atatcggctg ggtggtgggg cattcgtata tcgtctacgc gccgctgctg
     9061 gcggggatgg cgactatcgt ttacgaagga ttaccgacct ggccggactg cggcgtgtgg
     9121 tggaaaatcg tcgagaaata tcaggttagc cggatgttct cagcgccgac cgccattcgc
     9181 gtgctgaaaa aattccctac cgctgaaatt cgcaaacacg atctctcgtc gctggaagtg
     9241 ctctatctgg ctggagaacc gctggacgag ccgaccgcca gttgggtgag caatacgctg
     9301 gatgtgccgg tcatcgacaa ctactggcag accgaatccg gctggccgat tatggcgatt
     9361 gctcgcggtc tggacgacag gccgacgcgt ctgggaagcc ccggcgtgcc gatgtatggc
     9421 tataacgtgc agttgcttaa tgaagtcacc ggcgaaccgt gtggcgtcaa cgagaaaggg
     9481 atgctggtgg tggaagggcc gctgccgccg gggtgtattc agaccatctg gggcgacgac
     9541 gaccgctttg tgaagacgta ctggtcgctg ttttcccgcc cggtgtacgc cacctttgac
     9601 tggggcatcc gcgatgctga cggttatcac tttattctcg ggcgcactga cgatgtaatt
     9661 aacgttgccg ggcatcggct ggggacgcgc gagattgaag agagtatctc cagtcatccg
     9721 ggcgttgccg aagtggcggt ggttggggtg aaagatgcgc tgaaagggca ggtggcggtg
     9781 gcgtttgtca ttccgaaaga gagcgacagt ctggaagatc gtgatgtggc gcattcgcaa
     9841 gagaaggcga ttatggcgct ggtggacagc cagattggca actttggccg cccggcgcac
     9901 gtctggtttg tctcgcaatt gccaaaaacg cgatccggaa aaatgctgcg ccgcacgatc
     9961 caggcgattt gcgaaggacg cgatccgggg gatctgacga ccattgatga tccggcgtcg
    10021 ttggatcaga tccgccaggc gatggaagag taaggtattg tcggatgcgt cgcgtggcgc
    10081 atccgacagt tgtgcgcaga tgcctgatgc gacgctggcg cgtcttatca tgcctacagg
    10141 cctgaaccgt aggtcggata aggcgctcgc accgcatccg acaccgtgct cagtgcctga
    10201 tgcgacgcag gcgcgtctta tcat
//
LOCUS       DAZKFU020000172        10186 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZKFU020000172 DAZKFU020000000
VERSION     DAZKFU020000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.173"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            19..384
                     /gene="yfiL"
                     /locus_tag="ACHOQQ_RS17295"
                     /old_locus_tag="pgaptmp_003459"
     CDS             19..384
                     /gene="yfiL"
                     /locus_tag="ACHOQQ_RS17295"
                     /old_locus_tag="pgaptmp_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGU4471670.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            534..1052
                     /gene="yfiR"
                     /locus_tag="ACHOQQ_RS17300"
                     /old_locus_tag="pgaptmp_003460"
     CDS             534..1052
                     /gene="yfiR"
                     /locus_tag="ACHOQQ_RS17300"
                     /old_locus_tag="pgaptmp_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGU4471671.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            1042..2268
                     /gene="dgcN"
                     /locus_tag="ACHOQQ_RS17305"
                     /old_locus_tag="pgaptmp_003461"
     CDS             1042..2268
                     /gene="dgcN"
                     /locus_tag="ACHOQQ_RS17305"
                     /old_locus_tag="pgaptmp_003461"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGU4471672.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            2284..2766
                     /gene="yfiB"
                     /locus_tag="ACHOQQ_RS17310"
                     /old_locus_tag="pgaptmp_003462"
     CDS             2284..2766
                     /gene="yfiB"
                     /locus_tag="ACHOQQ_RS17310"
                     /old_locus_tag="pgaptmp_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4471673.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2843..3190)
                     /gene="rplS"
                     /locus_tag="ACHOQQ_RS17315"
                     /old_locus_tag="pgaptmp_003463"
     CDS             complement(2843..3190)
                     /gene="rplS"
                     /locus_tag="ACHOQQ_RS17315"
                     /old_locus_tag="pgaptmp_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGU4471674.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(3232..3999)
                     /gene="trmD"
                     /locus_tag="ACHOQQ_RS17320"
                     /old_locus_tag="pgaptmp_003464"
     CDS             complement(3232..3999)
                     /gene="trmD"
                     /locus_tag="ACHOQQ_RS17320"
                     /old_locus_tag="pgaptmp_003464"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGU4471675.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(4030..4578)
                     /gene="rimM"
                     /locus_tag="ACHOQQ_RS17325"
                     /old_locus_tag="pgaptmp_003465"
     CDS             complement(4030..4578)
                     /gene="rimM"
                     /locus_tag="ACHOQQ_RS17325"
                     /old_locus_tag="pgaptmp_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGU4471676.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4597..4845)
                     /gene="rpsP"
                     /locus_tag="ACHOQQ_RS17330"
                     /old_locus_tag="pgaptmp_003466"
     CDS             complement(4597..4845)
                     /gene="rpsP"
                     /locus_tag="ACHOQQ_RS17330"
                     /old_locus_tag="pgaptmp_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGU4471677.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4982..6343)
                     /gene="ffh"
                     /locus_tag="ACHOQQ_RS17335"
                     /old_locus_tag="pgaptmp_003467"
     CDS             complement(4982..6343)
                     /gene="ffh"
                     /locus_tag="ACHOQQ_RS17335"
                     /old_locus_tag="pgaptmp_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGU4471678.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6510..7301
                     /gene="ypjD"
                     /locus_tag="ACHOQQ_RS17340"
                     /old_locus_tag="pgaptmp_003468"
     CDS             6510..7301
                     /gene="ypjD"
                     /locus_tag="ACHOQQ_RS17340"
                     /old_locus_tag="pgaptmp_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGU4471679.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            7322..8608
                     /gene="yfjD"
                     /locus_tag="ACHOQQ_RS17345"
                     /old_locus_tag="pgaptmp_003469"
     CDS             7322..8608
                     /gene="yfjD"
                     /locus_tag="ACHOQQ_RS17345"
                     /old_locus_tag="pgaptmp_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HGU4471680.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSILLAPLQILMMPLVWLLNAITRMLMRIMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8663..9256)
                     /gene="grpE"
                     /locus_tag="ACHOQQ_RS17350"
                     /old_locus_tag="pgaptmp_003470"
     CDS             complement(8663..9256)
                     /gene="grpE"
                     /locus_tag="ACHOQQ_RS17350"
                     /old_locus_tag="pgaptmp_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGU4471681.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9379..>10186
                     /gene="nadK"
                     /locus_tag="ACHOQQ_RS17355"
                     /old_locus_tag="pgaptmp_003471"
     CDS             9379..>10186
                     /gene="nadK"
                     /locus_tag="ACHOQQ_RS17355"
                     /old_locus_tag="pgaptmp_003471"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGU4471682.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNL"
BASE COUNT         2495 a         2694 c         2403 g         2594 t
ORIGIN      
        1 ttgaaaaagg agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt
       61 agcggatgcc agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat
      121 gatgttggta tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc
      181 gattcacagg cggatcgcgg tctatacctt aaaggatatg ccgaaggaca aaagaaaact
      241 tgccagaccg attttactta tgcccgagga ctttccggta aaagctttcc tgccagctgc
      301 aacaatgttg aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat
      361 gccagcgcaa tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta
      421 ctcattatgt tttatttctc acaatgatga cgaccgcatg aaataatgat attttaatga
      481 gcattctgaa aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt
      541 tttctcaccg actgttctta ctgctgattc tcctgctgac gggagcacca atactcgctc
      601 aggagccttc tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca
      661 cccgctggcc tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta
      721 gtaccgccct acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca
      781 ctcaacaaga ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga
      841 cattccagat ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac
      901 aaaataccga gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca
      961 gatttgccgt aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct
     1021 taatgctcgc acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg
     1081 tttaaaagag cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc
     1141 tggttgctgc tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg
     1201 gcactgacag cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc
     1261 cctgcagcaa ctgaaacact ggcagcgctg ggccagcaag ggcaattttc aactgcagaa
     1321 gtacgtgata agcagcaaaa tattctggca tcctggcatt acacccgtaa ggatccaggc
     1381 gatactttca gcaatttcat aagccactgg ctcttccctg cccccatcat tcagccgatt
     1441 cgtcacaatg gtgaaaccat tggcgaagta cgcttaaccg ctcgcgacag ttcaatcagc
     1501 cattttatct ggttttcgct cgccgtactg accggttgta ttctgctggc atcaggaatc
     1561 gcaattaccc tcacccgcca tttgcacaat ggcctggtgg aagcactgaa aaatatcacc
     1621 gatgtcgtac atgatgtgcg ttccaaccgc aatttttccc gacgagtttc ggaagaacgt
     1681 atcgctgagt ttcaccgctt cgctctcgac ttcaacagtc tgctggatga aatggaagag
     1741 tggcagcttc gtttacaggc caaaaatgcg cagcttctgc gtaccgcgct acatgaccca
     1801 ttaaccgggc tggctaaccg cgcagcgttt cgtagcggca tcaatacgtt gatgaacaat
     1861 tccgatgccc gaaaaacgtc ggcgttacta tttcttgatg gcgataattt caaatatatc
     1921 aatgatacct ggggtcatgc gacgggcgat agagtcttga ttgaaatcgc aaaacggtta
     1981 gctgaatttg gcgggctgcg acataaagca taccgcctgg gcggcgatga attcgctatg
     2041 gtgctctatg atgtacagtc ggaatctgaa gtgcagcaga tatgctcagc actgacacaa
     2101 atctttaatc tcccgtttga tcttcataat ggccatcaga ccaccatgac attaagcatt
     2161 ggttacgcaa tgaccattgc gcacgcttct gcggaaaaat tacaagagct ggccgatcac
     2221 aatatgtatc aggccaaaca ccagcgtgcc gaaaagctgg tgagataaca aggatatatc
     2281 gatatgataa agcacctggt agcacccctg attttcacct cactaatact gactggctgc
     2341 cagtcccctc agggaaagtt tactcctgag caagtcgccg ctatgcaatc ttatggattt
     2401 actgaatccg ccggcgactg gtcgctgggc ttatcagatg ccattctgtt cgcaaaaaat
     2461 gactacaaat tgctcccgga aagccagcaa cagatccaaa ccatggcagc caaattggcc
     2521 tcgacagggc taacacatgc ccgtatggat ggacacaccg ataactatgg tgaagacagt
     2581 tacaacgaag gcttatcatt gaaacgggcg aatgtcgtgg ccgatgcatg ggctatgggt
     2641 ggacaaattc cacgcagcaa tctcaccaca cagggtttag gaaaaaaata tcccatagcc
     2701 agtaacaaga ccgcccaggg ccgcgccgag aaccgccgcg tcgcagtggt gattactacc
     2761 ccttaaaaca cgagcaaaca gataaaaaaa gggccagcca attggccagc ccttcttaac
     2821 aggatgtcgc ttaagcgaaa tcttagttaa gacgctcttt gatacgagca gccttaccag
     2881 tacgctcacg caggtagtac agtttagctt tacgaacagc accacgacgt ttgacagaaa
     2941 tgctgtcaac taccggagag tgagtctgga agacacgctc aacgccttcg ccgttggaaa
     3001 ttttacgaac agtgaatgca gagtgcagac cgcggttacg aatagcgata accacgccct
     3061 cgaatgcctg cagacgtttt ttggaacctt caacaaccca tactttcact tccacggtat
     3121 cacccggacg gaaggaaggt acgtcctgct tcatctgctc ttgttcaagt tgcttaataa
     3181 tgttgctcat aatttaatct cttatcctgg gtaaactgat atctcggggg cttacgccat
     3241 cccatcatgt ttatgttgct gttgtgcgtg ttccgttttg aactccgcca gcaaccttgc
     3301 ttgctcttca gtcagagcca ggttttccag aagttcaggt cttctaagcc aggtacggcc
     3361 cagcgactgt ttcaaacgcc agcgacgtat ctcggcatgg ttgcccgaca gtaacactgg
     3421 cggaacctcc atcccttcta acacctcagg ccgcgtatag tgcgggcaat ccagcaatcc
     3481 ttcagcaaag gaatcttccg ttgccgaggc ttcatgtccc agtactcccg gaataaaccg
     3541 ggaaacggag tcaatcagcg tcattgctgg taactcacca ccactgagaa cgtaatcgcc
     3601 gattgaccat tcttcgtcaa tttcggtttg gatcacgcgc tcatctatac cttcgtagcg
     3661 accgcacacc agaatcaatt tttgattcgt tgccagttcg ctgacgcccg cttgatcaag
     3721 cttgcgtccc tgtggtgaaa gataaatcac ctttgcgcct tcacccgccg cggcttttgc
     3781 tgcatgaatg gcgtcccgca agggttgcac catcattaac atccccggtc cgccgccgta
     3841 aggacgatcg tccacggtac ggtgccggtc atgcgtgaaa tcgcgaggac tccagctctg
     3901 gatgctcagc aggccatttt taactgcccg gccagttacc ccgtaatcgg taattgcgcg
     3961 gaacatttca ggaaacaggc taattatgcc aatccacata gcgccgtctt ttaccgttta
     4021 tccggtggtt taaaaaccag gatcccaatc tacttcgatt gaacgagtag tgagatcgac
     4081 tttcttgata acctgcccat cgaggaacgg tacgagacgt tccttgatac caaacgcatc
     4141 tttcaggttt gccttaatga cgagaacgtc attggatccg gtttccatca tatcgacgac
     4201 tttaccgaga tcgtagcctt cagtggttac tacctggcag cccatcaggt ctttccagta
     4261 gtagtcaccc tcttcaagct gaggcagctg cgatgaatcc acgacaattt cacaatttgt
     4321 cagcaggttc gccgcatcac gatcgtcaac gcctttcagc ttgatgatca tgtcctgatt
     4381 gtggtgcttc cagctttcca gctggacttg ctgccactga cccgccttct ggataaacca
     4441 gggctgatag tcaaaaatgc tttcggcgtc ttcggtggaa gaaaacactc tgagccaccc
     4501 acgaataccg taagacgaac ccatttttcc caaaacgatg ggatcaacag gtgcttgcgc
     4561 ggtgagttgt ttgctcatca tgaccaccgt gacagattaa gctgctttgt ttacttcttt
     4621 gatcagcgca gcaacgcgat cagaaatagt tgcgccctgg ccaacccagt gagcgatgcg
     4681 atccagatcc aggcgagtgc cttcttcttt ttcgctagcg attgggttga agaaaccaac
     4741 gcgctcgatg aagcgaccgt tgcgtgcatt acggctgtca gcgacaacaa cctggtagaa
     4801 cggacgcttt ttagcgccgt gacgtgctaa acgaatagtt accataacat cctcttgtgt
     4861 gaataaaaca accggacccc atcgaggaac ggagtccggt gtcatattaa aagcccgaaa
     4921 attttactga tttctggaga aaatgcaatc agcagttaat aactctgtcg cataaggtca
     4981 tttagcgacc aggaaatccc ggcggcatca tccccttcat gcttctcatc atcttcgcca
     5041 ttccgccctt cttcattttc ttcatcatgc gctgcatgtc gtcgaactgt ttcagaagac
     5101 ggttaacgtc ctgcacctgc ataccgcaac cggcagcaat acggcgttta cgcgaacctt
     5161 tgatgatttc tggcttagcg cgctctttca tcgtcatcga gttgatgatg gcttccatac
     5221 gcaccagcac tttatcgtcc atctgtgact tgacgttatc cgggatctgc cccatgcccg
     5281 gcagcttgcc catcagacta gccatgccgc ccatattttt catctggcgc agttgctcaa
     5341 gaaagtcgtt gagatcgaag ccgtcgcctt tttttagctt gctggctaat ttctctgcct
     5401 gcgcgcggtc aactttgctt tcgatatctt cgatcagcga cagtacgtcg cccatgccga
     5461 gaatacgcga cgcgatgcgg tccggatgga acggctccag cgcctcagtt ttctcgccaa
     5521 caccgaggaa cttgatcggt ttgccagtga tgtgacgaat agagagcgcc gcaccgccgc
     5581 gggcatcgcc gtccactttg gtcaacacta cgccggtcag cggtaacgct tcattgaatg
     5641 cttttgccgt attggccgca tcctgaccgg tcatggcgtc aaccacaaac agggtttcaa
     5701 ccggatttat ggacgcatgg acttgtttga tctcgtccat catcgcttcg tcaacgtgca
     5761 gacgaccagc ggtatccacc agcagcacgt cgtagaattt cagtttggct tctttcagcg
     5821 ccgcgttaac gatatctacc ggcttctgac caacatcaga agggaagaaa tcaacgccca
     5881 cctgctctgc aagcgtctca agctgtttga ttgccgctgg acgataaacg tcggcagaaa
     5941 ccaccagcac ttttttcttg tgcttctcgc gcaggaactt accgagctta ccgacactgg
     6001 tggttttacc ggcaccttgc aggcccgcca tcagcacgac cgcaggcggt tgtgccgcca
     6061 gattcagggt ctggttctct tcgcccatcg ccgcaaccag ttcgttacgg acgattttga
     6121 cgaactcctg ccccggcgtc aggcttttat taacttcatg accaaccgct ttctctttta
     6181 cgcgattgat aaactcacgc actaccggca gagcgacgtc cgcctccagc agcgccatgc
     6241 gcacttcgcg cagcgtatct tttacgttgt cttcagtgag gcgtccacgg ccactgatat
     6301 tgcgcagcgt gcgcgacaaa cgatcggtta aattatcaaa cattgtctct cgcctggggt
     6361 ggaaacggtt ggccgcaatc gcgacacatc atcagtattt tgccgcagta taacatgaag
     6421 gcgtctttgt tgttatgcaa cggttggagc agcgttcacc tgacgctata ctgcttctct
     6481 ttcttattgc tcaaactgtc gacattacta tgcccgtttt tgctctgctc gcgcttgtcg
     6541 cctactccgt cagtcttgcg ctgattgttc ccggtctgct gcaaaaaaac ggcggctggc
     6601 ggcgcatggc tattatttct gcggtcattg cgctggtctg ccacgcaatc gctctggaag
     6661 cccgcatcct gcccgacggt gatagcggac aaaacctcag cctgctgaac gttggttcat
     6721 tggtcagttt gatgatctgt acggtaatga ccattgtggc ttctcgcaat cgtggctggt
     6781 tgctgctacc cattgtctat gcctttgcgc ttatcaacct ggcgctggca accttcatgc
     6841 ccaatgaata catcacccat ctggaagcta cacctgggat gctggtgcac attggcttat
     6901 cgctcttttc ctatgccacg ctaattatcg ccgccctgta cgcgctgcaa ctggcgtgga
     6961 ttgattacca actgaagaac aagaagctgg cgtttaacca ggaaatgccg ccattgatga
     7021 gtatcgagcg taaaatgttc cacatcacgc agattggcgt ggtgctgcta acgctcacgc
     7081 tttgcactgg cctgttctac atgcacaact tatttagcat ggaaaatatc gacaaggctg
     7141 tgctctctat cgtggcgtgg tttgtctata ttgtgctgct gtggggacat tatcatgaag
     7201 gatggcgtgg acgccgcgtc gtctggttta acgttgcggg tgcagtcatt ctgacactgg
     7261 cctacttcgg cagccgaatt gtccagcagt taatcagcta aacccagaaa aggagtttcc
     7321 cctggaacac atttctacta ctacgttgat cattattctg atcatcatgg tggtcatttc
     7381 agcctatttt tccgggtccg aaaccggaat gatgaccctc aaccgctatc gtctgcgaca
     7441 tatggcgaaa cagggtaatc gctcggccaa acgcgtcgaa aaattgctgc gtaagccaga
     7501 ccgcctgata agcctggtgt taatcggcaa taacctggtc aatattcttg cctccgcgct
     7561 cggcactatt gttgggatgc gtttgtacgg cgatgcgggc gtggcaattg cgactggtgt
     7621 gctgaccttt gtggtgctgg tttttgccga agtgttgccg aaaactatcg ctgcgctata
     7681 cccggaaaaa gtcgcttatc cgagtagtat tctgttagca ccgctgcaaa ttttgatgat
     7741 gccgctggtc tggttgctga acgctatcac ccgcatgtta atgcgcatta tgggcatcaa
     7801 aaccgatatc gtggtcagcg gctctttgag caaagaagag ttgcgcacta tcgtgcacga
     7861 atcgcgctca caaatttccc gtcgcaatca ggatatgctg ctgtcggtgc tcgatctgga
     7921 aaaaatgacc gttgatgaca tcatggtgcc gcgcagtgaa attatcggta ttgatatcaa
     7981 cgatgactgg aaatctattc tgcgccaact ctcccactca cctcacgggc gcatcgtgct
     8041 ctaccgtgat tcgctggacg acgccatcag tatgctgcga gtacgtgaag cctggcggtt
     8101 gatgtcggag aaaaaagagt tcaccaaaga aaccatgctg cgcgccgcgg acgagatcta
     8161 ttttgtgccg gaaggtacgc cgctcagcac gcagttggta aagtttcagc gcaacaaaaa
     8221 gaaagtcggc ctggtcgtca acgagtatgg agacattcaa gggctggtga cggttgaaga
     8281 tattctggaa gagattgtcg gcgatttcac tacgtcgatg tcgccaacac ttgccgaaga
     8341 ggtcacgccg caaaacgacg gttcggtgat tatcgatggc accgccaacg tgcgggaaat
     8401 caacaaagcc tttaactggc atctaccgga agatgatgcc cgcacggtta atggcgtcat
     8461 tcttgaggca ctggaagaga tccctgtcgc aggcacccgc gtgcgtattg gcgagtacga
     8521 tatagatatt ctcgacgtac aggacaatat gattaagcag gtaaaagttt ttcctgtgaa
     8581 accgctgcgc gagagtgtgg cggagtaacg aaaacggccc tgcattcgca tgcagggccg
     8641 tgaattatta cgaaaacaga aattacgctt ttgctttcgc tacagttacc atcgccgcac
     8701 gaatcgtacg accattcagc gtataaccct tctgcataat gcccagtacg ttacctggcg
     8761 caacgtcatc agattccacc attgcgatag cctgatgcac attcgggtcc agcggcacgt
     8821 tagtttcggc gatcacttca acaccaaact tacgcacaac atccagcatc gacttcagcg
     8881 tcagctcaat gccttcaacc atcgcagaca tatccgggtt agctttatca gccacttcca
     8941 gcgcacgatc caggctatca atcaccggca gcaattcgtt gatgaatttc tccagcgcga
     9001 atttgtgggc tttttcaata tccagttcag tacgacgacg caggttttcc atttcggctt
     9061 ttacacgcaa aatgccgtca cgttcacggg tctgggcttc agccagctga gcttcgagat
     9121 tcgcaatttt ttcatcgcgc ggatccacct gctcagcaga agcttctggc tcaactgcct
     9181 caatctcttc gtgctgatcc atgataattt cttccggggc ttgcccctca ggcgttttct
     9241 gttctttact actcatgaat ttctccgcgt ttttttcgca ttcatctcgc taacttcgct
     9301 tattatgggg atcagtttca gggtttcaag ggaagcactc acattgtcat caatcttcgc
     9361 aacaaggacc tcggaaaaat gaataatcat ttcaagtgta ttggcattgt gggacaccca
     9421 cggcacccta ctgcactgac aacacatgaa atgctctacc gctggctgtg cacaaaaggt
     9481 tacgaggtca tcgttgagca acaaatcgct cacgaattgc aactgaagaa tgtgaaaact
     9541 ggcacgctcg cggagattgg gcaactggct gatctcgcgg tagtcgttgg tggcgacggt
     9601 aatatgctgg gcgcggcgcg cacgctcgcc cgttacgata ttaaagttat tggaatcaac
     9661 cgtggcaacc tgggtttcct gactgacctt gaccccgata acgcccagca acaattagcc
     9721 gatgtgctgg aaggtcacta catcagcgag aaacgttttt tgctggaagc gcaagtctgt
     9781 cagcaagatt gccagaaacg catcagcacc gcgataaatg aagtggtact tcatccaggc
     9841 aaagtggcgc atatgataga gttcgaagtg tatatcgacg agatctttgc gttttctcag
     9901 cgatctgatg ggctaattat ttcgacgcca acaggctcca ccgcctattc cctctctgcg
     9961 ggcggtccta ttctgacccc ctctctggat gcgattaccc tggtgcccat gttcccgcat
    10021 acgttgtcag cacgaccact ggtcataaac agcagcagca cgatccgtct gcgtttttcg
    10081 catcgccgta acgacctgga aatcagttgc gacagccaga tagcactgcc gattcaggaa
    10141 ggtgaagatg tcctgattcg tcgctgtgat taccatctga atctga
//
LOCUS       DAZKFU020000173        10130 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZKFU020000173 DAZKFU020000000
VERSION     DAZKFU020000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.174"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            276..2426
                     /gene="btsT"
                     /locus_tag="ACHOQQ_RS17360"
                     /old_locus_tag="pgaptmp_003472"
     CDS             276..2426
                     /gene="btsT"
                     /locus_tag="ACHOQQ_RS17360"
                     /old_locus_tag="pgaptmp_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGU4471683.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2476..2679
                     /locus_tag="ACHOQQ_RS17365"
                     /old_locus_tag="pgaptmp_003473"
     CDS             2476..2679
                     /locus_tag="ACHOQQ_RS17365"
                     /old_locus_tag="pgaptmp_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4471684.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2690..3646
                     /gene="yjiA"
                     /locus_tag="ACHOQQ_RS17370"
                     /old_locus_tag="pgaptmp_003474"
     CDS             2690..3646
                     /gene="yjiA"
                     /locus_tag="ACHOQQ_RS17370"
                     /old_locus_tag="pgaptmp_003474"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGU4471685.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILMTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3692..4606)
                     /gene="mrr"
                     /locus_tag="ACHOQQ_RS17375"
                     /old_locus_tag="pgaptmp_003475"
     CDS             complement(3692..4606)
                     /gene="mrr"
                     /locus_tag="ACHOQQ_RS17375"
                     /old_locus_tag="pgaptmp_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine/cytosine-specific restriction
                     endonuclease Mrr"
                     /protein_id="HGU4471686.1"
                     /translation="MTVPTYDKFIEPVLRYLATKPEGAAARDVHEAAADALGLDDSQR
                     AKVITSGQLVYKNRAGWAHDRLKRAGLSQSLSRGKWCLTPAGFDWVASHPQPMTEQET
                     NHLAFDFVNVKLKSRPDAVDLDPKADSPDHEELAKSSPDDRLDQALKELRDAVADEVL
                     ENLLQVSPSRFEVIVLDVLHRLGYGGHRDDLQRVGGTGDGGIDGVISLDKLGLEKVYV
                     QAKRWQNTVGRPELQAFYGALAGQKAKRGVFITTSGFTSQARDFAQSVEGMVLVDGER
                     LVHLMIENEVGVSSRLLKVPKLDMDYFE"
     gene            4794..8306
                     /gene="hsdR"
                     /locus_tag="ACHOQQ_RS17380"
                     /old_locus_tag="pgaptmp_003476"
     CDS             4794..8306
                     /gene="hsdR"
                     /locus_tag="ACHOQQ_RS17380"
                     /old_locus_tag="pgaptmp_003476"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
                     /protein_id="HGU4471687.1"
                     /translation="MMNKSNFEFLKGVNDFTYAIACAAENNYPDDPNTTLIKMRMFGE
                     ATAKHLGLLLNIPPCENQHDLLRELGKIAFVDDNILSVFHKLRRIGNQAVHEYHNDLD
                     DAQMCLRLGFRLAVWYYRLVTKDYDFPVPVFVLPERGENLYHQEVLTLKQQLEQQVRE
                     KAQSQAEVEAQQQKLVALNGYIAILEGKQQETEAQTQARLAALEAQLAEKNAELAKQT
                     EQERKAYHKEITDQAIKRTLNLSEEESRFLIDAQLRKAGWQADSKTLRFSKGARPEPG
                     VNKAIAEWPTGKDETGNQGFADYVLFVGLKPIAVVEAKRKNIDVPGKLNESYRYSKCF
                     DNGFLRETLLEHYSPDEVHEAVPEYETSWQDTSGQQRFKIPFCYSTNGREYRAAMKTK
                     SGIWYRDVRDTRNMSKALPEWHRPEELLEMLGSEPQKQNQWFADNPGMSELGLRYYQE
                     DAVRAVEKAIVKGQQEILLAMATGTGKTRTAIAMMFRLIQSQRFKRILFLVDRRSLGE
                     QALGAFEDTRINGDTFNSIFDIKGLTDKFPEDSTKIHVATVQSLVKRTLQSDEPMPVA
                     RYDCIVVDEAHRGYILDKEQTEGELQFRSQLDYVSAYRRILDHFDAVKIALTATPALH
                     TVQIFGEPVYRYTYRTAVIDGFLIDQDPPIQITTRNAQEGVYLSKGEQVERISPQGEV
                     INDTLEDDQDFEVADFNRGLVIPAFNRAVCNELTNYLDPTGSQKTLVFCVTNAHADMV
                     VEELRTAFKKKYPQLEHDAIIKITGDADKDARKVQTMITRFNKERLPNIVVTVDLLTT
                     GVDIPSICNIVFLRKVRSRILYEQMKGRATRLCPDVNKTSFKIFDCVDIYSTLESVDT
                     MRPVVVRPKVELQTLVNEITDSETYKITEADGRSFAEHSHEQLVAKLQRIIGLATFNR
                     DRSETIDKQVRRLDELCQDAAGVNFNGFASRLREKGPHWSAEVFNKLPGFIARLEKLK
                     TDINNLNDAPIFLDIDDEVVSVKSLYGDYDTPQDFLEAFDSLVQRSPNAQPALQAVIN
                     RPRDLTRKGLVELQEWFDRQHFEESSLRKAWKETRNEDIAARLIGHIRRAAVGDALKP
                     FEERVDHALTRIKGENDWSSEQLSWLDRLAQALKEKVVLDDDVFKTGNFHRRGGKAML
                     QRTFDDNLDSLLDKFSDYIWDELA"
     gene            8383..9972
                     /gene="hsdM"
                     /locus_tag="ACHOQQ_RS17385"
                     /old_locus_tag="pgaptmp_003477"
     CDS             8383..9972
                     /gene="hsdM"
                     /locus_tag="ACHOQQ_RS17385"
                     /old_locus_tag="pgaptmp_003477"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     methyltransferase"
                     /protein_id="HGU4471688.1"
                     /translation="MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETG
                     QEAEYLPVGYRWDDLKSRIGQEQLQFYRNLLVHLGADNQKLVQAVFQNVNTTITQPKQ
                     LTELVSNMDSLDWYSGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII
                     HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELV
                     PSTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTN
                     ITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHL
                     HTILRLPTGIFYAQGVKTNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNMPSFGKRTP
                     FTDEHLQPFERVYGEDPHGLSPRTEGEWSFNAEETEVADSEENKNTDQHLATSRWRKF
                     SREWIRTAKSDSLDISWLKDKDSIDADNLPEPDVLAAEAMGELVQALGELDALMRELG
                     ASDEADAQRQLLEEAFGGVKE"
BASE COUNT         2470 a         2741 c         2683 g         2236 t
ORIGIN      
        1 taatttttaa atgagttaca caaccgaggg ctaaaaatcg cttcggttgt gatcatcatc
       61 acataaataa ccttcataaa ccttgctcaa aaccgctcaa ccgcaaaact gaccgcttac
      121 gtccctaaaa taaccactca gttatttacc ttactttacg cgcgcgtaac tctggcagca
      181 tcactgcagg atagcggtca atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc
      241 ggccaactat taatcaatac atgccaggtt ttactatgga tacgaaaaaa ctattcaagc
      301 acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta gttgcattac
      361 gtcgggggga gcacgtcagt gccctgtgga tcgtggtcgc ctctgtgtcg gtgtatctgg
      421 tggcgtatcg ctactacagt ttgtatatcg cccagaaggt gatgaaactc gaccccacgc
      481 gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta cgttccgacc aaccgttacg
      541 tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtt ggtccggttc
      601 tcgccgcgca gatgggctac ctgcctggca cactgtggct gctggcgggg gtagtgctgg
      661 ccggtgcggt acaggacttt atggtgctgt ttatctcctc tcgccgtaac ggcgcatctc
      721 tcggtgagat gatcaaagaa gagatgggac cagtaccggg gactatcgcg ctgtttggct
      781 gtttcttaat catgatcatc atccttgccg tcctggcgct gattgtggtt aaagccctgg
      841 ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accaattgcg ctgtttatgg
      901 gtatctacat gcgctttatc cgtccggggc gtgtgggtga agtctctgtc attggtatcg
      961 tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg tactggggtc
     1021 cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat gcgtttgttt
     1081 ccgcgctgct gccggtgtgg ctgatcctcg caccacgcga ctatctggca accttcctga
     1141 aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg gaactgaaaa
     1201 tgcctgcgat gacccagtac attgacggta ctggcccgct gtggaaaggc gctctgttcc
     1261 cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgca ctgatctctt
     1321 ccggtactac gccgaaactg ctggctaatg aaaccgacgc gcgtttcatc ggttatggcg
     1381 caatgctgat ggagtccttc gtggcgatta tggcgctggt tgcagcgtcc atcatcgaac
     1441 cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc atgcctaacc
     1501 tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagttaaaa gacgttaccg
     1561 cacacgcggc ggcgaccgtc agctcctggg gcttcgtgat ttcgccagag cagatcctgc
     1621 aaaccgcgaa agacattggt gagccttctg tcctgaaccg tgcaggtggt gcgccgacgc
     1681 tggcggtagg tatcgctcac gtgttccaca aagtgctgcc gatggctgac atgggcttct
     1741 ggtatcactt cggtattctg ttcgaagccc tgttcatcct gaccgcgctg gatgcgggta
     1801 cccgttctgg ccgctttatg ctgcaagatc tgctgggtaa cttcatcccg ttcctgaaaa
     1861 aaaccgactc tctggttgcc ggtatcatcg gtactgcggg ctgtgtgggt ctgtggggct
     1921 acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg tggccgctgt
     1981 tcggtatttc caaccagatg ctggcagccg tagcgctggt actgggcacc gttgtgctga
     2041 ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta tggctgctta
     2101 tctgcaccac ctgggcgctg ggtctgaaac tgttcagtac caacccgcag atggaaggct
     2161 tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac ctgacggcgc
     2221 agcagattgc caacatgaac cacatcgttg tgaacaacta caccaacgca ggcctgagta
     2281 ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc tggcttgcgg
     2341 tgcgtaacag cgacaaacgt actgacaaag aaacaccgta cgttccaatc ccggaaggcg
     2401 gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg ggctttgttc
     2461 ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct cggccaggcg
     2521 gcaaagatgt tgattggcat tccagactac gacaactacg tcgagcatat gaagaccaac
     2581 catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca gaacgcgcgc
     2641 tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca tgaacccgat
     2701 tgcagttacc ctactcaccg gttttttagg cgcgggaaaa accaccctgc tgcgccatat
     2761 tcttaacgaa caacacggct acaagattgc cgtgattgaa aacgaattcg gcgaagtctc
     2821 cgttgatgat caactgattg gcgaccgcgc cacgcagatc aaaacgctga ccaacggctg
     2881 catctgctgt tcgcgctcca acgagctgga agacgcacta ctcgacctgc tggataatct
     2941 cgacaagggc aatattcagt tcgaccgtct ggtcattgaa tgcaccggca tggccgatcc
     3001 cggcccgatt attcagacct ttttctccca tgagatttta tgccagcgtt acctgctgga
     3061 cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc agttcaccat
     3121 cgcccagtcc caggtgggct acgccgaccg tattctgatg accaaaaccg acgtcgcagg
     3181 cgaagcagaa aaactgcgtg aacgcctggc gcgcatcaac gcccgtgcac cggtctacac
     3241 cgttacccac ggcgacatcg acctgggcct gctgttcaac accaacggtt ttatgctgga
     3301 agaaaacgtc gtcagcacca aaccgcgttt ccactttatc gcggataaac aaaacgatat
     3361 ttcgtcgatt gtggtagaac tggattaccc ggtagatatc agcgaagttt cccgggtgat
     3421 ggaaaacctg ctgctggaat cggcggataa actactgcgt tacaaaggga tgctatggat
     3481 tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag cgcctctaca gcgccgactg
     3541 ggaccgcccg tggggcgatg aaaaaccgca tagcacgatg gtgtttatcg ggattcagct
     3601 gccggaggaa gagattcggg cggcgtttgc ggggttgagg aagtaagggg ttatgggccg
     3661 gataaggcgc agccgcatcc ggcctgatat ttcactcaaa atagtccata tccagtttcg
     3721 gcaccttcaa caaacgtgaa gaaaccccta cttcgttttc gatcattaag tgcaccaggc
     3781 gttccccatc aaccaacacc ataccctcga cggattgagc aaagtcacgc gcctgagacg
     3841 taaatccaga agtggtaata aacaccccac gtttcgcctt ttgcccagcc agtgcgccgt
     3901 aaaatgcctg taattctggc ctgcctacag tgttctgcca acgttttgcc tgaacataaa
     3961 ctttctccag gccaagttta tcaagcgata tcacaccatc gatgccacca tctccagtac
     4021 cgccaacacg ctgcaaatca tcacggtggc cgccataccc caggcgatgc aaaacatcca
     4081 gaacaatgac ttcaaagcgc gaaggagaaa cctgcaataa gttttccaga acctcatcag
     4141 ccaccgcatc acgaagctct tttagcgcct gatctaaccg atcgtccggg ctgctctttg
     4201 caagttcttc atgatcggga gagtcggctt tcggatctaa atcgacggca tccggccgtg
     4261 acttaagttt gacattcaca aaatcgaagg ccagatggtt cgtctcctgc tccgtcattg
     4321 gctggggatg agacgcaacc cagtcaaaac ccgcaggagt caggcaccat ttgccacgcg
     4381 acaaactttg cgacaacccg gcacgtttta aacggtcatg cgcccagcct gcacgatttt
     4441 tataaacaag ttgtccgctg gtaatgactt tcgctcgctg gctgtcatcc agtcctaatg
     4501 catccgcggc agcctcatga acatcacgcg cggctgcacc ttccggtttt gtcgccagat
     4561 aacgcagaac aggttcaata aatttgtcat aggtaggaac cgtcatagta catccttgca
     4621 gaatcaggta gatgtttttc ggctactata acactaccaa aatagacgaa cacgttagaa
     4681 atgagtcagt tgctgtgacc gtggtcattg cccggaaagg tacagaaagc taagataagg
     4741 cgttatgggc cttaaatatt tggacaggcc cacacagcaa tggattaata acaatgatga
     4801 ataaatccaa ttttgaattc ctgaagggcg tcaacgactt tacctatgcc atcgcctgtg
     4861 cggcggaaaa taactacccg gatgatccca acacgacgct gattaaaatg cgtatgtttg
     4921 gcgaagccac agcgaaacat cttggtctgt tactcaacat ccccccttgt gagaatcaac
     4981 acgatctcct gcgcgaactc ggcaaaatcg ccttcgttga tgacaacatt ctctctgtat
     5041 tccacaaatt acgccgcatt ggtaaccagg cggtgcacga atatcataac gatctcgacg
     5101 atgcccagat gtgcctgcga ctcggcttcc gcctggctgt ctggtactac cgtctggtca
     5161 ccaaagatta tgacttcccg gtaccggtgt ttgtgttgcc ggaacgtggc gaaaacctct
     5221 atcaccagga agtgctgacg ctaaaacaac agcttgaaca gcaggtgcga gaaaaagcgc
     5281 agagtcaggc agaagtcgaa gcacaacagc agaagctggt tgccctgaac ggctatatcg
     5341 ccattctgga aggcaagcag caggaaaccg aagcgcaaac ccaggctcgc cttgctgcac
     5401 tggaagcaca gctcgccgag aagaacgcgg aactggccaa gcagaccgaa caggaacgta
     5461 aggcttacca caaagaaatt accgatcagg ccatcaagcg tacactcaat cttagcgaag
     5521 aagagagccg cttcctgatt gacgcgcaac tgcgtaaagc aggctggcag gccgatagca
     5581 aaaccctgcg cttctccaaa ggcgcgcgcc cggaacccgg cgtcaataaa gccattgccg
     5641 aatggccgac cgggaaagat gaaacgggta atcagggctt tgcggattat gtgctgtttg
     5701 tcggtctcaa acccatcgcg gtggtagaag cgaaacgtaa aaatatcgac gttcccggca
     5761 agctcaatga gtcgtatcgc tacagtaaat gtttcgataa tggcttcctg cgggaaacct
     5821 tgcttgagca ctactcaccg gatgaagtgc atgaagcggt gccggagtat gaaactagtt
     5881 ggcaggacac cagcggccaa caacggttta aaatcccctt ctgctactcg accaacgggc
     5941 gcgaataccg cgcagcgatg aagactaaaa gtggcatctg gtatcgcgac gtgcgtgata
     6001 cccgcaatat gtcgaaagcc ttacccgagt ggcatcgccc ggaagagttg ctggaaatgc
     6061 tcggcagcga accgcaaaaa cagaatcagt ggtttgccga taatcccggc atgagcgagc
     6121 tgggcctgcg ttattatcag gaagatgccg tccgcgcggt tgaaaaggca atcgtcaagg
     6181 gtcaacaaga gatcctgctg gcgatggcga ccggtaccgg taaaacccgc accgcaattg
     6241 ccatgatgtt ccgcctgatc cagtcccagc gttttaaacg cattctcttc cttgtggatc
     6301 gccgttctct tggcgagcag gcgctggggg cgtttgaaga tacgcgtatt aacggcgaca
     6361 ctttcaacag cattttcgac attaaagggc tgacggataa attccccgaa gacagcacca
     6421 aaattcacgt tgccaccgta cagtcgctgg tgaaacgcac cctgcaatcg gatgaaccga
     6481 tgccggtagc ccgttacgac tgtatcgtcg tcgatgaagc gcatcgcggc tatattctcg
     6541 ataaagagca gaccgaaggc gaactgcagt tccgcagcca actggattac gtttctgcct
     6601 accgtcgtat tctcgatcac ttcgatgcgg taaaaattgc tctcaccgcc accccggcgc
     6661 tgcatacggt gcagattttc ggcgagccgg tttaccgtta tacctaccgt accgcggtta
     6721 tcgacggttt tctgatcgac caagatccgc cgattcaaat caccacccgc aacgcccagg
     6781 aaggggttta tctctccaaa ggcgagcagg ttgagcgcat cagcccgcag ggggaagtga
     6841 tcaacgacac cctggaagac gatcaggatt ttgaggtcgc cgactttaac cgtggcctgg
     6901 tgatcccggc gtttaaccgc gccgtctgta acgaactcac caattacctt gacccgaccg
     6961 ggtcacaaaa aacgctggtc ttctgcgtca ctaatgctca tgccgatatg gtggtggaag
     7021 agctgcgtac cgcattcaag aaaaagtatc cgcaactgga gcacgacgcg atcatcaaga
     7081 tcactggtga tgccgataaa gacgcgcgca aagtgcagac catgatcacc cgcttcaata
     7141 aagagcggct gcccaatatt gtggtaaccg tcgacctgct gacgaccggc gtcgatattc
     7201 cgtcgatctg taacatcgtg ttcctgcgta aagtacgcag ccgtattctg tacgagcaaa
     7261 tgaaaggccg cgccacgcgc ttatgcccgg acgtgaataa aaccagcttt aagattttcg
     7321 actgcgtcga tatctacagc acgctggaga gcgtcgacac catgcgtccg gtggtggttc
     7381 gcccgaaggt ggaactgcaa acgctggtca acgaaattac cgattcagaa acctataaaa
     7441 tcaccgaagc ggacggccgt agctttgccg agcacagcca tgaacaactg gtggcgaagc
     7501 tccagcgcat catcggtctg gccacgttta accgtgaccg cagcgaaacg atagataaac
     7561 aggtgcgtcg tctggatgag ctatgccagg acgcggcggg cgtgaacttt aacggcttcg
     7621 cctcgcgcct gcgggaaaaa gggccgcact ggagcgccga agtctttaac aaactgcctg
     7681 gctttatcgc ccgtctggaa aagctgaaaa ccgacatcaa caacctgaat gatgcgccga
     7741 tcttcctcga tatcgacgat gaagtggtga gtgtaaaatc gctgtacggt gattacgaca
     7801 cgccgcagga tttcctcgaa gcctttgact cgctggtgca acgttccccg aacgcgcaac
     7861 cagcattgca ggcggtgatt aatcgcccgc gcgatctcac ccgtaaaggg ctggtcgagc
     7921 tacaggagtg gtttgaccgc cagcactttg aggaatcttc cctgcgcaaa gcgtggaaag
     7981 agacgcgcaa tgaagatatc gccgcccggc tgattggaca tattcgccgc gctgcggtgg
     8041 gcgatgcgct gaaaccgttt gaggaacgtg tcgatcacgc gctgacgcgc attaagggcg
     8101 aaaacgactg gagcagcgag caattaagct ggctcgatcg tttagcgcag gcgctgaaag
     8161 agaaagtggt gctcgacgac gatgtcttca aaaccggcaa cttccaccgt cgcggcggga
     8221 aggcgatgct gcaaagaacc tttgacgata atctcgacag cctgctcgat aaattcagcg
     8281 attatatctg ggacgagctg gcctgacacg gtatcattcc cggcaatttc ttcttctgcg
     8341 gcccaataaa tttgggccgc tgcttaattt acggaacacc caatgaacaa taacgatctg
     8401 gtcgcgaagc tgtggaaact gtgcgacaac ctgcgcgatg gcggcgtttc ctatcaaaac
     8461 tacgtcaatg aactcgcctc gctgctgttt ttgaaaatgt gtaaagagac cggacaggaa
     8521 gcggagtacc tgccggtagg ctaccgctgg gatgacctga aatcccgcat cggccaggag
     8581 cagttgcagt tctaccgtaa cctgctggtg catctgggcg ccgacaatca aaagctggtg
     8641 caggcggtgt tccagaacgt caacaccacc attacccagc caaaacagtt gaccgaactg
     8701 gtcagcaata tggattcact ggactggtac agcggcgcgc acggtaagtc gcgcgatgac
     8761 ttcggcgata tgtacgaagg tctgttgcag aagaacgcga acgaaaccaa gtctggcgcg
     8821 ggccagtact tcaccccgcg tccgctgata aaaaccatta ttcatctgct gaaaccacag
     8881 ccgcgtgaag tggtacagga cccggcagca ggtacggcgg gctttttgat tgaagccgac
     8941 cgctacgtta agtcgcaaac caatgatctg gacgaccttg atggtgacac gcaggatttc
     9001 cagatccacc gcgcgtttat cggcctcgaa ctggtgccta gcacccgtcg tctggcgctg
     9061 atgaactgcc tgctgcacga tattgaaggc aacctcgacc acggcggcgc aatccgtctg
     9121 ggcaacaccc tgggtagcga cggcgaaaac ctgccgaagg cgcatattgt cgccactaac
     9181 ccgccgtttg gcagcgccgc aggcaccaac attacccgca cctttgttca cccgaccagc
     9241 aacaaacagt tgtgctttat gcagcatatt atcgaaacgc tgcaccccgg cggtcgtgcg
     9301 gcggtggtgg tgccggataa cgtgctgttt gaaggcggca aaggcaccga catccgtcgt
     9361 gacctgatgg ataagtgtca tctgcacact attctgcgtc tgccgaccgg tattttttac
     9421 gctcagggcg tgaagaccaa cgtgctgttc tttaccaaag ggacggtggc gaacccgaat
     9481 caggataaga actgtaccga tgatgtgtgg gtgtacgacc tgcgtaccaa tatgccgagc
     9541 tttggtaaac gcacgccgtt taccgacgag catttgcagc cgtttgaacg ggtgtatggc
     9601 gaagacccgc acggcttaag cccccgtact gaaggtgaat ggagttttaa cgctgaagag
     9661 acggaagttg ccgacagcga agagaacaaa aacaccgacc agcacctggc caccagccgc
     9721 tggcgcaagt tcagccgtga gtggatccgc accgcgaaat ccgattcgct ggatatctcc
     9781 tggctgaaag ataaagacag cattgatgcc gacaacctgc cggagccgga tgtattagcg
     9841 gcagaagcga tgggcgaact ggtacaggca ctgggtgaac tggatgcgct gatgcgtgaa
     9901 ctgggggcga gcgatgaggc ggatgcgcag cgtcagttgc tggaagaagc gtttggtggg
     9961 gtgaaggaat gagtttaaaa aatattccaa cgaattggtg tgtaactacc ataggggata
    10021 ttaatgacta taaatcatta acttttaatc ccaaatcata ccaggaggac agcttcgaat
    10081 tgtatagcgt tcccagcttt gcatctggct caccagaaat attactggga
//
LOCUS       DAZKFU020000174        10117 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZKFU020000174 DAZKFU020000000
VERSION     DAZKFU020000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.175"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..152)
                     /locus_tag="ACHOQQ_RS17390"
                     /old_locus_tag="pgaptmp_003478"
     CDS             complement(<1..152)
                     /locus_tag="ACHOQQ_RS17390"
                     /old_locus_tag="pgaptmp_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4471689.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAG"
     gene            complement(368..748)
                     /locus_tag="ACHOQQ_RS17395"
                     /old_locus_tag="pgaptmp_003479"
     CDS             complement(368..748)
                     /locus_tag="ACHOQQ_RS17395"
                     /old_locus_tag="pgaptmp_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4471690.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(766..1755)
                     /locus_tag="ACHOQQ_RS17400"
                     /old_locus_tag="pgaptmp_003480"
     CDS             complement(766..1755)
                     /locus_tag="ACHOQQ_RS17400"
                     /old_locus_tag="pgaptmp_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4471691.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRLAPGAIRLCWHCDNLLRDQFTERLEAMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(1807..2065)
                     /locus_tag="ACHOQQ_RS17405"
                     /old_locus_tag="pgaptmp_003481"
                     /pseudo
     CDS             complement(1807..2065)
                     /locus_tag="ACHOQQ_RS17405"
                     /old_locus_tag="pgaptmp_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2062..3462)
                     /locus_tag="ACHOQQ_RS17410"
                     /old_locus_tag="pgaptmp_003482"
     CDS             complement(2062..3462)
                     /locus_tag="ACHOQQ_RS17410"
                     /old_locus_tag="pgaptmp_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4471692.1"
                     /translation="MTNPTNPAWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFF
                     VRQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWAKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMTSLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRHIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTQGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKAERLF"
     gene            complement(3459..4349)
                     /locus_tag="ACHOQQ_RS17415"
                     /old_locus_tag="pgaptmp_003483"
     CDS             complement(3459..4349)
                     /locus_tag="ACHOQQ_RS17415"
                     /old_locus_tag="pgaptmp_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4471693.1"
                     /translation="MTNFLNKQHMQHDKAQLLNRKADLEEELAFVRGGKRPWRCEHWV
                     QSTETVSCGKHGKYTRYVLTGPDVRGKAVKRVSGCLSCLSEELDRVYDELRALKVREL
                     LDQAGIARRFQDCEFENYQAVNTDAQKNLEACQRYAASWKKCLAAGTSMVMIGNCGTG
                     KNHLAVSMAKNIIRHHQATIEITDVMRLTRAVKNTWRHNSERTDDEVINHFASLDLLI
                     IDEVGVQFGTPTEITILQEIINARYESVLPTILISNLTFEQLKESIGERIVDRVTDGG
                     RNCLVFGWESYRAHIRGVAA"
     gene            complement(4369..5280)
                     /locus_tag="ACHOQQ_RS17420"
                     /old_locus_tag="pgaptmp_003484"
     CDS             complement(4369..5280)
                     /locus_tag="ACHOQQ_RS17420"
                     /old_locus_tag="pgaptmp_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4471694.1"
                     /translation="MSSKILGNVWDACAAYGIKGAKLMIMARLADYSNDDGVCYPGVE
                     TICRQLGLGESTVRTAISELEADGWLTRQSRRKGNRNTSNLYHLNADRLELLARTERD
                     KVAELKQQRRLSGLRDPSDSEPSKSEPSESVCSGVFDPSDSGKNTRLTLQNLTPDPQG
                     LKHEPPVNSKHEPQDIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFK
                     AWKARIREGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEESWQQPA
                     APGGGRGKSLPISGFSEQDYGSTNFNW"
     gene            complement(5277..5501)
                     /locus_tag="ACHOQQ_RS17425"
                     /old_locus_tag="pgaptmp_003485"
     CDS             complement(5277..5501)
                     /locus_tag="ACHOQQ_RS17425"
                     /old_locus_tag="pgaptmp_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471695.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGHVLRVCLVNIESQKVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGAV"
     gene            complement(5498..6361)
                     /locus_tag="ACHOQQ_RS17430"
                     /old_locus_tag="pgaptmp_003486"
     CDS             complement(5498..6361)
                     /locus_tag="ACHOQQ_RS17430"
                     /old_locus_tag="pgaptmp_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4471696.1"
                     /translation="MTRHQHIDSASPGRYTVPAPYKTGAGIGVLKLITEHNRAHAVFS
                     CHEHYYAQIMVGRTGPTSVGPGSVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMT
                     TLPTLSQPEIAIVDGQAVTSSLAVADFFSKRHDDVLKKIRILDCSPEFCARNFAETSI
                     LVRQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMEKSLSGADAS
                     DMSAVARNARGVYLHLREIHQIWTSQLYPMLKAVESPLASKLYDRVGDAVFGAALVDS
                     RLNGSDKEVRP"
     gene            complement(6358..6990)
                     /locus_tag="ACHOQQ_RS17435"
                     /old_locus_tag="pgaptmp_003487"
     CDS             complement(6358..6990)
                     /locus_tag="ACHOQQ_RS17435"
                     /old_locus_tag="pgaptmp_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HGU4471697.1"
                     /translation="MKFRRHTLHIVAKSTIGSETPELLQATNDAPASFLLSASLHARI
                     MVGWAGEPKGSPVSCNAGSLNPVQSATSEIETSGGGTVPLLQEAALMATIPAHPYLKI
                     EVVNGRAVIFSLYVACHFKRMHKNIIDRIEYLNCSREFLTRNFIPGTYRAYGDSVRGY
                     YITRDGLMMLQLGLSLRTMRYYESCIEAFHEAEAVQGHSVFRRNQREVHQ"
     gene            complement(7019..7195)
                     /locus_tag="ACHOQQ_RS17440"
                     /old_locus_tag="pgaptmp_003488"
     CDS             complement(7019..7195)
                     /locus_tag="ACHOQQ_RS17440"
                     /old_locus_tag="pgaptmp_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001664303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4471698.1"
                     /translation="MQVLNRWFRDGRGRRVHVIRWESETQRVIYLRDGYPHECFIPLW
                     LFRRDFVECEAPPT"
     gene            complement(7215..7922)
                     /locus_tag="ACHOQQ_RS17445"
                     /old_locus_tag="pgaptmp_003489"
     CDS             complement(7215..7922)
                     /locus_tag="ACHOQQ_RS17445"
                     /old_locus_tag="pgaptmp_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4471699.1"
                     /translation="MNTTIFTDKASMTSVEIAELVGKRHDNVKRTIETLAKGGVVRSP
                     QIEVSERINNLGFKVQHEHYLFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRLERVEEKVSHVTETVEQIKRGAIRDGYAGYRQLAAK
                     TGMSDAKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMSAFHRMTSEAEPRGT
                     RWYHPKMGLFQALGWQV"
     gene            complement(7919..8182)
                     /locus_tag="ACHOQQ_RS17450"
                     /old_locus_tag="pgaptmp_003490"
     CDS             complement(7919..8182)
                     /locus_tag="ACHOQQ_RS17450"
                     /old_locus_tag="pgaptmp_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4471700.1"
                     /translation="MKNEGIAKAVDIAGSQIALARRCGRAQSTICDWLNGKKKISPEF
                     VPSLVKAVDGKVQAYEFRPDLPELFPHPSLAHTEDGVCEGDKQ"
     gene            8306..9010
                     /locus_tag="ACHOQQ_RS17455"
                     /old_locus_tag="pgaptmp_003491"
     CDS             8306..9010
                     /locus_tag="ACHOQQ_RS17455"
                     /old_locus_tag="pgaptmp_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4471701.1"
                     /translation="MSFSDRLQLAMTIRGYSQGKLAREVGMAQSSVNKLVNGATGSRK
                     VVEIANTLNVRPEWLSYGVGPMTPEESTSCNPTPQVSYSPLENDKDIFRVEVLDIAAS
                     AGPGTFLVSDFAETVHAIEFSHDAARRLFCSRPANIIKMITVDGDSMAPTLCAGDQVF
                     VDVSVRNFETDGIYIFIFGHTFHIKRLQMQGMQLAVISDNPAYKEWFISESAEEHLFI
                     MGKVLIHQSIQYNRVG"
     gene            9270..9857
                     /locus_tag="ACHOQQ_RS17460"
                     /old_locus_tag="pgaptmp_003492"
     CDS             9270..9857
                     /locus_tag="ACHOQQ_RS17460"
                     /old_locus_tag="pgaptmp_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4471702.1"
                     /translation="MSENGAESERVYNAIRCLMRSLQKTEETASIFAEKLCEAKAMVS
                     HGHFFGKIRAQREACGLTTTELAKLLDLDEEIILQWESGEFEPTISMLIPLANVIGCD
                     PLSLLDEKDSASVIRVNAPEVYVESIGARIKSARKKLGLTEADLARMINTYSDPINDW
                     ECGICEVPADQIVPLASALNCDLMWLLTGKSEAKE"
     gene            9863..10063
                     /locus_tag="ACHOQQ_RS17465"
                     /old_locus_tag="pgaptmp_003493"
     CDS             9863..10063
                     /locus_tag="ACHOQQ_RS17465"
                     /old_locus_tag="pgaptmp_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471703.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHDLLCAVVVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
BASE COUNT         2520 a         2702 c         2389 g         2506 t
ORIGIN      
        1 cccgccataa caagctgggt cagtattaac tggcagcgtt cacgtgaaag gtacgtattc
       61 tgcgcaatct ccccgactgt cgccggttcg gtgacgctta attcattaaa caccactctg
      121 gcggtttctg tcatatcctg ctgtttcagc atgtcttttt cccttttccg gttaacgtga
      181 cacaccaata actcttgtcg aaaaagccag caagctgaaa gaccggtatt caccgccacc
      241 agcgcgttta ctgtactggc gtgatttcag tcataaaaaa acccgctcgc ggcgggttta
      301 agctgtgtgg cgaagtaacc actcttaaca tactgacata ctttttgcgg accgcactaa
      361 tcatttttta cttttttagc agccagtcgt ccatctccag tcttaccccc agcacagaca
      421 aacatccgtc aataaaccct tcggctatct gcatctcaat tcgtattgct ttttcgcttt
      481 tctttctcgt cctggctatc tgtctttttg atattcgcaa caaataatga gcaatgagaa
      541 gcgaatactc ctcaggtttt ttctgcttca gacgagcaag acagttttca atgataagtc
      601 cgtcatcatc gcagcaggcc ggacgtggtt tagtggcaga tggtaaaagt cctttgaatc
      661 cggcagcgat cggagaatag tccaccccgg tgttaccact tgccgcccat gccccccagc
      721 gttcaagaac catctgaata tcacgcatca actttctcca caaaatcagg acagcacacc
      781 aatcgccagt gcgcgatcga taaaacgaaa tatcagctcc agttgggaac catacttctc
      841 ttcaaatgcc acagtatccg catgcagttc gtcgtggtgt tttctgcaca aaggcagcac
      901 aaaaaggtca tgcgcttttg tccccatacc accctgaccg tgaccaatca gatgatgcgg
      961 atcatcggct ggctttccac aacacgcgca cggctgcgtc ttaacccagc gagtgtactt
     1021 ttcattaacc cagcgacgac gtttcgggcg taacataaaa gactccggcg actccggatc
     1081 cactttcagc gccagcacct ttttcgcttt atcccggatg atgctggtgg cgggaaccga
     1141 aggcacaagg tcactttccc gggtgacaga cggcacaaca ggcttcggta atctcagtgc
     1201 cttacgggct gcactttccg gtaaggcatc cgccaggtca ttacgaacca gccaccagca
     1261 cagttccggc attgtcacaa cgtgactgtc atcaaaacca agatcacggc gcacaacaga
     1321 caacacccag cgggcacagt tatccgttgc cattgcttcc agccgttccg tgaactggtc
     1381 acggagaaga ttgtcacagt gccagcacag acggattgca cccggcgcga gccgcattgt
     1441 ggtcatgttc tcgctgtgcc atccggaatg aggccactga cagccctttt cacgaagtaa
     1501 ccagctttca agacattcca ccccaccagc acgacgaata actgcctcat tgcggaacac
     1561 ggcccgcaca gcaggatcat ccgctaacgg ttgtgatgcc gccggaacgg caccactggc
     1621 aaaagatgaa taacgttccg gctcaggctc cagcaggaca cgcccctgca taaacagggg
     1681 catcagctct gagcctggtc tgaacagtac gatccccatt cgcggggcaa tttcaggagt
     1741 caacagtgct ctcacggtca cctcagcgaa cgatattgca tgaacacagg agaaaaaatt
     1801 cagccatcac gcagtaaact ccttcaccag cgtttcaaac tggcttacct tgccttccag
     1861 ttccgccacg caatccacca gctcatccac tgccttttgt gtgcggtgtt ttgcctgcag
     1921 cagatcacga agcgccggag taagctgctt gcggagcgta tcctttgcca cgctcatttt
     1981 tttccatctg ttcagcacaa cgaagcatct cctgcgcctg gcgacgaagt tgttccggtg
     2041 aaacagtggc tgttctgtta ttcaaaataa acgctccgct ttactgcccg acatgcggtt
     2101 attgctgtat ctgcgcggat tgcccggcgt catgggtgtg gaaagaaccc gggcactctc
     2161 ctggtccaca ggcagaaaat gcccgttatg aaaacgccgg taaatggtac cctgcgtgcc
     2221 attacgctgt ttcgtgatgt tgatttctgc tatgcctctc gcctgtgtct ccgggttgta
     2281 cacctcatcc ctgtaaagca tcagaatgat gtctgcatcc gcctctattt ccccggagtt
     2341 tttcaggtcc gagttcatgg ggcgtttatt gggtctggat tccacaccgc gggagagctg
     2401 gctcagcgct atcagcggga aaccgccgga ttttgccagg cttttaagtc cctttgagat
     2461 ttcccccaca gcaaggtcgt gacgccccgt actacgggtt ttaatcaggc cgaggtaatc
     2521 gaccaccacc agcgccgttt ccgggtgttt catccggtgg tgcctcgtgg ttgcacatat
     2581 ctcatcaatg gtcaggtttg cctggtccac catccagata tgacgccccg tcattcgtcc
     2641 cacgccctgt gagaaacgcg cccagtcttc atcttcaaaa cgggcaacag acttaagacg
     2701 ggataccggc attcccccgg cagcagacac catacgttca ccaatctgga tgttcgccat
     2761 ctccatggtg aacagaagca cgccatgccc ctgctcagtc accttgtcga tgatatccag
     2821 cgcaagttcg gttttcccca tcgaaggacg agccgcaatg aataccaggt cgcctggctc
     2881 cataccgccc gtttttgcgt ccagttcatc aataccggtc atcagtgccc tggatttctc
     2941 cagtccctga ttgcggcatt caacacggtc gaccacttcc ggaaggacat catcaatgtg
     3001 aaccggctga atgacgccct ttccggtcga cagtgaggtc atcatgttct gcgcatcctt
     3061 cagggcatcc tcagctgctt cacaggtatg cgcatcacgt aaattctgta atgcttcggt
     3121 cagtgttttt tctgcatcgc gcagtgcggc attgcgccgc agcgctgcaa catagtgctc
     3181 cagtgaagac ttcgcccagg ttttgcgtcc ggtgtcggta atcaccgggg caagttccgg
     3241 catctcattg cacagcagta cggggtcaat gacgccggat acacgggcct gtctgcaaat
     3301 tcccgcgtaa atatccctgt actgacgcac gaaaaagaca tccgccggaa gtgtggccag
     3361 aatatccatc acttccggat cagccccacg cagaaaaaac gcgccaatga cagctccttc
     3421 caggtcatca ttacgccacg ccggatttgt cgggtttgtc atgctgccac acctctgata
     3481 tgcgcacggt agctttccca accaaacacc aggcagttac gcccaccatc agtaacgcga
     3541 tccacaatcc gttcaccaat ggattcctta agctgttcaa acgtcaggtt gctgatcaaa
     3601 attgtcggta aaacgctttc gtaacgcgca tttatgattt cctgcaggat ggttatctct
     3661 gtaggcgtgc cgaactgcac accaacctca tcgatgataa gcagatccag cgatgcaaaa
     3721 tgattaatca cttcatcgtc agtgcgctca gagttgtggc gccaggtatt tttcaccgca
     3781 cgggtaagcc gcatcacatc cgtgatttct atggttgcct ggtggtggcg aataatattt
     3841 tttgccattg atacagccag gtgatttttc ccggtaccac aattgccaat catgaccatg
     3901 ctggtgcctg cagcgagaca tttcttccag gaagcggcat agcgctgaca ggcttcaagg
     3961 tttttctgtg cgtcagtatt caccgcctga taattctcaa actcacagtc ctggaaccgg
     4021 cgggcaatac cggcctgatc gagtaattca cgaaccttca gggcgcgtaa ttcgtcatac
     4081 acgcggtcca gctcctcact gaggcaggac aggcaaccgg acacccgttt gacagctttc
     4141 ccccttacat ccgggcccgt cagcacatag cgcgtgtatt ttccgtgttt cccgcaggac
     4201 accgtctcag tgctttgcac ccaatgctca cagcgccacg gacgctttcc gccacggaca
     4261 aatgccagct cctcttccag gtctgccttg cggttaagta actgcgcttt gtcgtgttgc
     4321 atgtgttgtt tgttcaggaa attagtcatt ttcaccctcc ccacaaaatc accagttgaa
     4381 gttcgttgag ccgtagtcct gttcactgaa tcccgagatc gggaggcttt tgccccgccc
     4441 acctccgggg gctgctggct gttgccagga ttcctcgaaa tgacgatcgg gtccaaagaa
     4501 cgtcgacgcc tgcttcacga actgggtacc ggtatttcca gagacacgca cccaggcggc
     4561 atagcgtttc acaccgttga gcatggtttc gggtgtcaca ccttccctga ttcgggcttt
     4621 ccaggctttg aaggctgctg acttggaatt accaccagca cgtttgggat attcctgcca
     4681 ggcctgttca aattccggtg aatattcctg tctggcagaa cgcgctggtg cagacgcgtc
     4741 agcggatgcg ccaatatctt gcggttcatg ttttgaattt actggtggtt catgttttaa
     4801 accttgtgga tctggggtca gattctgaag ggtcaaacgc gtatttttgc cagaatctga
     4861 agggtcaaac acacctgaac atacagattc tgacggttca gattttgaag gttcagaatc
     4921 tgaagggtca cgtaatcctg aaagtctgcg ctgctgtttc agttctgcaa ccttatcccg
     4981 ctcagttctg gcaagcagct caagccgatc agcattcaga tgataaagat tggacgtatt
     5041 acggttacct ttgcggcgtg actgacgcgt cagccagcca tcagcctcca gttcggagat
     5101 tgccgttctg actgtacttt ctcccaaccc aagctgtcgg catatggttt caacacctgg
     5161 gtagcacacc ccgtcatcat tcgaataatc cgccaagcgc gccataatca tcagttttgc
     5221 acctttgatg ccatatgctg cacatgcatc ccagacatta cctaggattt tgctactcat
     5281 acggcacctc ccagacgctt aaacattttt ccggactgaa acgccaccag cggataactc
     5341 agggtatgag tacgtccctg aacctggcag acaaccttct ggctttctat attgaccagg
     5401 caaacccgca gaacgtgtcc gttgctggtg gtgaaccact gccccacacg ggggcaatgg
     5461 ttgtatcggt gatacaggaa attaacgatg tggcggatca tggacgcacc tccttgtcag
     5521 aaccattcag cctggaatca acaagtgcag caccaaaaac agcatcacct acacggtcgt
     5581 acagtttgct ggccagcgga gattcaacag ccttaagcat tggataaagc tggcttgtcc
     5641 agatttgatg gatttcacgc aaatgcaggt atacgcctct ggcgtttcgt gcgacagctg
     5701 acatatcaga cgcatcggca cctgataaac tcttctccat ctggttaaag gcattgatgt
     5761 atgcctcttt gaaccgggca gcacgtttac cagtgaagcc catagcaaga aacgcaaagc
     5821 cgtcgcgggt tatttgatag caaggaagtt tgcgagtacc gccgttgggc tggcgtacca
     5881 aaattgatgt ctccgcaaaa ttgcgggcac aaaactctgg agaacaatcc aaaatgcgga
     5941 tctttttcag aacatcgtca tgacgtttag agaagaagtc agcaacagcc aaagaagatg
     6001 taacagcctg accatcaacg atggcaattt caggttgaga gagggttggg agagtagtca
     6061 tagtgacagc cccggtagtc agttttttag aaaactcacc acatgggacg ccaatcacag
     6121 aggtggtgag acgtacaggg ttggcgttac cggagactac cgaacccggc ccgaccgaag
     6181 tcggccctgt acgccccacc ataatttggg cgtagtaatg ctcatgacac gaaaaaaccg
     6241 catgagcgcg gttatgctca gtaatcaatt tcaggacgcc aatcccggca cccgttttat
     6301 aaggtgccgg aacagtgtaa cgtcccggac ttgcagaatc aatatgctgg tggcgcgtca
     6361 ttgatgcacc tcccgttgat tacgacggaa cacggagtga ccctggacgg cttcagcctc
     6421 atggaatgct tcaatgcagc tctcgtagta ccgcatggtg cgcagactta acccaagctg
     6481 tagcatcatc agaccatcac gggtgatgta ataaccacgc actgaatcgc cataggcgcg
     6541 ataagtgccg ggaataaagt tgcgggtgag aaattcgcgt gagcagttga gatattcgat
     6601 tcgatcgatg atattcttat gcatgcgctt aaagtgacag gcgacataca gggagaatat
     6661 aacggctctg ccgttgacga cctcgatttt gaggtatgga tgtgcgggga tcgtagccat
     6721 taaggcagcc tcctgtaaca gtggaacagt tccaccaccg gaggtctcaa tctcgctggt
     6781 ggcggactgg acagggttga gactaccggc gttacaggaa accggcgaac ctttcggttc
     6841 ccctgcccag cccaccataa ttctggcgtg caaggacgct gacaacaaaa aagacgctgg
     6901 cgcgtcattt gtcgcctgta acaattccgg ggtctcactc ccgatagtgg attttgccac
     6961 tatatgtaaa gtgtgccgcc ggaatttaat tttgtcaacg aggttaaata tagagtcgct
     7021 atgttggtgg cgcttcgcac tcaacaaaat cacgacgaaa caaccacagc gggatgaagc
     7081 attcatgcgg ataaccatca cgcaggtaaa taacccgctg tgtttcagac tcccagcgta
     7141 taacgtggac gcgacgcccc cttccatcac ggaaccagcg attgagtact tgcatgtgtt
     7201 acctgtgagc ataattacac ctgccagccc agcgcctgga acaaccccat tttcgggtga
     7261 taccagcgag tacctcgtgg ttctgcttca ctcgtcatgc gatgaaaagc agacatgaag
     7321 ggttccacag caacaatagc gcgacgcgat aacaatccat ccggcgtcat gaactcatgg
     7381 gtgtcggtag ggatctgata tgcgttcacc agattgcggc atttcgcatc tgacataccc
     7441 gttttcgccg ccagctgacg atagcctgca taaccatcgc gtatggcgcc tcttttgatt
     7501 tgttcgacag tttcggtaac gtgactgact ttttcttcta cccgctcaag gcgtttctgc
     7561 tgacgaacag cttcaagagc catcgcggca accatttcga tttcgctcat tggcttgcgg
     7621 atctgttctt ccagttcgcg ccagcggtct accagacggg ctgtgaattc agggcaaagc
     7681 tgtgcgacaa caatgatgct gtcgcgtttg ccttgttcgc cttcaaacag ataatgctcg
     7741 tgttgaactt taaaccctaa gttattgatt ctttcggaaa cctcaatttg aggagatcgg
     7801 acaacaccac ctttagctaa tgtttcaata gtgcgtttca cattgtcatg acgcttaccc
     7861 accagctctg cgatctcaac gctggtcatg gatgctttat cggtaaaaat tgttgtattc
     7921 actgtttatc tccttcgcac actccatctt ctgtgtgcgc taaacttggg tgtgggaaaa
     7981 gctccggtag atcaggacga aattcatacg cctggacttt cccatcgacc gccttaacca
     8041 gagacggaac aaattcagga gaaatttttt tcttcccatt cagccagtcg caaattgttg
     8101 actgcgcgcg accacaacgc ctagccagag caatctggct tcccgcaata tctacagcct
     8161 tggctatacc ttcatttttc atatcgacct ccaacctgat tccattcact atagcgattg
     8221 attaaagcga ttgcaatgta cgggaaagcg gtttgtgtat atatcgctaa ggcgatacac
     8281 taaaagggca gaaaaaggag gacttatgag cttttcagac aggctacagt tagctatgac
     8341 tatccgcgga tactcgcagg gaaaactggc cagagaggtt ggcatggctc aatcgagcgt
     8401 aaataaactg gttaatggtg ccacaggctc caggaaagtt gtggagatag ccaataccct
     8461 gaacgtgcgc cccgagtggc tatcgtacgg tgttggtcct atgaccccag aagaaagcac
     8521 atcctgcaat ccgacaccac aggtatcata ttccccgctg gaaaatgata aggatatttt
     8581 tcgtgtggaa gtcctggata tcgctgccag cgcgggacca ggaacattcc ttgtttcaga
     8641 ttttgcagaa acggtccatg ctatagaatt ttcacatgac gccgcaagac gtctgttctg
     8701 tagcaggcca gcaaatatca taaagatgat aacagttgat ggcgacagca tggctcctac
     8761 tctgtgtgca ggagatcagg tgtttgtcga cgtatccgtc agaaattttg aaactgacgg
     8821 tatctatatt tttatttttg ggcacacttt ccatatcaaa cgcctgcaaa tgcaagggat
     8881 gcaacttgct gttatatcag acaatccggc ctacaaggaa tggtttataa gtgaatcagc
     8941 tgaagaacat ctattcataa tggggaaagt tctcattcat cagtcaatcc agtataacag
     9001 ggtcggataa gtcaacatca ccggcagctg tattgcgcag ctgctctact ttcctgtatc
     9061 tcaactccct acgtatcgtc tcatttctct cataatctgt tctttgctgc ccaaaatgca
     9121 tatctatagc gattgacata accaaacgct ttggctattg taaaattaaa acaacaggag
     9181 gctcatatga agcatgcagt taaggtgatg gatgaaattc agcacaaata caagaaagcg
     9241 gctcgctgct tggaatgatc tattcctaca tgtcagaaaa cggagcagaa agtgagaggg
     9301 tttataatgc aatcagatgc ctgatgcgtt ccctgcaaaa aacagaagaa actgcatcaa
     9361 tttttgcaga aaaactatgt gaggcaaaag ctatggtttc gcatggtcac ttttttggaa
     9421 aaatccgtgc ccaacgtgaa gcctgcggct tgacgaccac cgaacttgcc aaactgcttg
     9481 atctcgatga agagatcatc ctgcaatggg agagcggaga gttcgaaccg accatcagca
     9541 tgcttatccc cctggcaaac gtcattgggt gcgatccgct ttctttgctg gatgaaaaag
     9601 atagcgcgtc tgttattcgc gtaaatgcgc ctgaagtcta cgtggaaagt attggcgcac
     9661 gcatcaaaag cgcacgtaaa aaactgggct taacggaagc tgatcttgcc cgcatgatta
     9721 atacctatag cgaccccata aacgactggg aatgcggcat ctgtgaagtt cctgctgatc
     9781 agatagtgcc actggccagt gcgcttaatt gtgacctgat gtggttgtta acgggaaaat
     9841 cagaagcaaa ggagtagcaa caatgactgg taatatccat gataagtatg aaggcttatg
     9901 cctagcacca gattccttcg caaacaatat ccatgattta ttatgcgcag ttgtcgtatt
     9961 acaaatgtca gacaacgacg caataaaaag aacaggtgat gaagttcttg aatttgcacg
    10021 ttgctatgct gaagcagcag ctgaaaaaga actatccagt taaatagaac aagtcatctc
    10081 cagatactat attacggctt aatcgccggg gattatc
//
LOCUS       DAZKFU020000175        10050 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZKFU020000175 DAZKFU020000000
VERSION     DAZKFU020000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.176"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..5176
                     /gene="iatD"
                     /locus_tag="ACHOQQ_RS17470"
                     /old_locus_tag="pgaptmp_003494"
                     /pseudo
     CDS             1..5176
                     /gene="iatD"
                     /locus_tag="ACHOQQ_RS17470"
                     /old_locus_tag="pgaptmp_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(5300..5884)
                     /gene="kptA"
                     /locus_tag="ACHOQQ_RS17475"
                     /old_locus_tag="pgaptmp_003495"
     CDS             complement(5300..5884)
                     /gene="kptA"
                     /locus_tag="ACHOQQ_RS17475"
                     /old_locus_tag="pgaptmp_003495"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HGU4471704.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            6134..6817
                     /gene="yjiH"
                     /locus_tag="ACHOQQ_RS17480"
                     /old_locus_tag="pgaptmp_003496"
     CDS             6134..6817
                     /gene="yjiH"
                     /locus_tag="ACHOQQ_RS17480"
                     /old_locus_tag="pgaptmp_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGU4471705.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            6814..7275
                     /gene="yjiG"
                     /locus_tag="ACHOQQ_RS17485"
                     /old_locus_tag="pgaptmp_003497"
     CDS             6814..7275
                     /gene="yjiG"
                     /locus_tag="ACHOQQ_RS17485"
                     /old_locus_tag="pgaptmp_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGU4471706.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLTTAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            7288..8460
                     /gene="iadA"
                     /locus_tag="ACHOQQ_RS17490"
                     /old_locus_tag="pgaptmp_003498"
     CDS             7288..8460
                     /gene="iadA"
                     /locus_tag="ACHOQQ_RS17490"
                     /old_locus_tag="pgaptmp_003498"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4471707.1"
                     /translation="MIDYNAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            8525..9436
                     /gene="hypT"
                     /locus_tag="ACHOQQ_RS17495"
                     /old_locus_tag="pgaptmp_003499"
                     /gene_synonym="qseD"
     CDS             8525..9436
                     /gene="hypT"
                     /locus_tag="ACHOQQ_RS17495"
                     /old_locus_tag="pgaptmp_003499"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HGU4471708.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(9429..9821)
                     /gene="iraD"
                     /locus_tag="ACHOQQ_RS17500"
                     /old_locus_tag="pgaptmp_003500"
     CDS             complement(9429..9821)
                     /gene="iraD"
                     /locus_tag="ACHOQQ_RS17500"
                     /old_locus_tag="pgaptmp_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGU4471709.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLVISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(9818..9901)
                     /gene="idlP"
                     /locus_tag="ACHOQQ_RS17505"
                     /old_locus_tag="pgaptmp_003501"
     CDS             complement(9818..9901)
                     /gene="idlP"
                     /locus_tag="ACHOQQ_RS17505"
                     /old_locus_tag="pgaptmp_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGU4471710.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         2622 a         2561 c         2493 g         2374 t
ORIGIN      
        1 caaaatacgc cgcctgaccg ccggagtttg tttatttaca caaatttttt tccccgtcat
       61 ggcgacggca caaaatgtgg tacatgctaa acctcagacg acagtttcat ctgccccccc
      121 tctaatagaa aataatacgg tgccttacac gcttggtgcg ctggaatcag cgcaaagcgt
      181 tgctgatcga ttcggtattt cactggagga gcttcgtcgt cttaatcagt tccgtacttt
      241 tgctcgcggc tttgataacg tgcgccaggg tgaggaactg gatgttccgg caacaacctc
      301 gcagaaaagc catgagcaac aaaatgccat accacctgcg aatggcgaaa acacgctgga
      361 gaatcaaata gccagcacct cgcagcgagt tggcactctg ctttcacaag atatgaatag
      421 tgagcaggcc agcggcatgg cgcgtggttg ggcgtcttca gaagcctcag gcgcgatgac
      481 tgattggtta aataactttg gtactgcgag aatctctctg ggtgtggatg aagattttag
      541 cctgaaaaat tcgcaattcg acttcctgca tccgtggtat gacacacctg attatctgct
      601 cttcagccag catacccttc accgaacaga cgatcgtacc cagatcaaca ccggtttggg
      661 ctggcgtcat ttcacctcca gctggatgtc aggcatcaac ctgttttttg accacgacct
      721 gagccgctat cactcccgcg cagggcttgg cgcagaatac tggcgtgatt atctgaagtt
      781 gagcagcaac gcttatatcg gcctgaccgg ctggcgtagc gcaccagaat tggataacga
      841 cttcgaagcc cgcccggcca acggctggga tttacgcgcg gaaggctggt tacctgcctg
      901 gccacaactg gggggaaaac tggtctatga acaatactat ggcgatgaag tggcgctgtt
      961 tgacaagaat gatcgtcaaa gtaaccccca tgctattacc gcaggcctca actatacccc
     1021 cttcccgctt ctgactctca gtgcggaaca gcgtcagggg aagcaaggtg aaaatgacac
     1081 acgttttgcc gttgatctga cctggcaacc cagcagttca atgcagaaac agcttaaccc
     1141 ggacgaagtg gccggacggc gcagtctggc cggtagccgt tatgacctga ttgatcgcaa
     1201 caacaacatc gttctggaat atcgcaagaa agagctgatt cgcctgagtc tgctggatcc
     1261 agtgaaaggg aagtctggag aaataaaacc gctggtttcc tcgctacaga ccaaatatgc
     1321 cctgaaaggc tataacatcg aagccgctgc gctggaagct gccggaggta aagtcagcac
     1381 gtctggaaaa gatatcacgg tcacgctgcc aggttaccgc ttcactaaca ccccagaaac
     1441 cgataataca tggtcgatag acgttaccgc cgaggatgta aaaggtaacc tgtctcggca
     1501 tgaacaaagc atggtagtta ttcaggctcc gacattaagc cagaaagatt ctctattatc
     1561 cgttaatccg ctaaccgtgg ctgcagataa aaaatcgacg accacattga ccgttactgc
     1621 gcacgattcc gacggaactc cggtgccggg gctggcgctg caaacccgca gtgaaggcgt
     1681 tcaggatatc accctgtctg actggacaga taacggtgat ggtagttaca tacagatgct
     1741 gaccgccgga acgacatcag gttcagtaac actgacgccg caaattaacg gtgagagtgc
     1801 ggtaaaagaa tccatcgtcg ttaatatcgt ccctgttgtc tcatcccgcg accattcatc
     1861 aataacaatt gataacgtat cgtattatgc cggagacgac atcaaggtta gggtggaact
     1921 gaaagacgaa agtaatcaac cggttgcata tcaaaaagag gaattggtaa aagccgttac
     1981 tgtcgaaaac agcaaacctg gcaccacgat tgtctggcac gaagagcagc cgggcgttta
     2041 taccgcgaat tatccggccc ataagcaagg gactgcacta agggcacaac ttagccttca
     2101 caactggaat gctccactgc aatcgcatat ttataacatt gaggcaaacc agaataaggc
     2161 tcgcgtcgcc acattatcag cgacaaataa tgacgtttac gccgataaaa agacatttaa
     2221 taccctcact atcaacgtca ctgatgagag tgataatccc ctgacaaatc atcaggtcac
     2281 ctttaagaat gaaaaaggaa gcgcggaatt tgtcgaaccg ccgcagcaaa atacggatgc
     2341 atatggtgtt gccacaatca acatggtaag tcaggttgcg gaagaaaata cgattagcgc
     2401 cacgctgcca aatggtttct cacaacggat aattgcgaaa tttgttagcg attcgagtac
     2461 gccaaaattc aaacaactgg ttgccgatcc agataccatt attgctggca acagccaggg
     2521 cagtactctg accgccaccg tcacagactt tcataacaac ccgttaaaag atatgaaagt
     2581 gaattttgtg gcacctggtg gctcgcaact ggacaacacg tccgccacaa cagaccagtc
     2641 cggtattgtg cgggtgcacc tgaccagttc aaaagctggt agctattccg tcgatgcctc
     2701 gcttgaggtg gataaaaata ttcaccagtc ggtcacgatc accgtggtcc caaacaggga
     2761 acaatcggta atgaccttga atgccgggtc gggcagtgcg atcgctaaca atacaaatac
     2821 cgttaccctg actgccagtg tgaaagatgt ttatggacac ccgttgccgg atgaggatgt
     2881 gaaatttacc ttgccagcct ccatgaccgg gaacttcacg ctaagtagtg aaaccgcccg
     2941 caccgatgca aacggtgatg ccgtggtcac attgcgaggc acaaaagcgg gtgagtttac
     3001 agttacggcg acgctgacca gaaataatac cgttgcttat cagcaagtca cttttattgg
     3061 ggatacaaac agtgcgcagc tccagccgct gactgcctca ttaaattcca ttgttgcggg
     3121 taacagtacg gggagtaccc tgacggcaac gatcctggac gcttaccaaa atccgcttaa
     3181 agaccagtag gtcacattcc agagtaacga tgtcactcta agcggaacag aagtcaccac
     3241 caatacgcta ggtcaggcga cggtaacaat gaccagcaat attgccgggc aacataacgt
     3301 cgtggtgagc cggaaagcgc aagcctccga taataaaacg tttagtttat cagtgctccc
     3361 ggatgaaagt tcggcgaagg taataagtat aaccggagcc gaaaaaacga taacggttgg
     3421 cgaaaacatc acgctacgga tactcgtcca ggacgcgttt aacaatgtaa tagcgggtca
     3481 acgcgtcaga ttaagtgcgc agccaacagc taacattacg ataggcgata cggcttacac
     3541 cgataataac ggttatgcgt acgttaacct tattagcacc cagcctgggg tttatcaggt
     3601 gacggcaacg ctggacaata acagtagtag taaggttgac gtgaatgtgg caaatggcaa
     3661 actcgagtta acatcatcga aaccagaaac cacggtccat aatagtgagg gtattacgct
     3721 taccgcaacg gcgagaaatg cgcggggtga attgatgcca gggcaaatta tcacctttag
     3781 cgtaacgcct gaaggtgcaa cgctaagcaa tacaggggaa gtccttactg accagtcagg
     3841 tcaggccaaa gtgacgctga ccagtgacaa agtgaatgtc tataccgtta cggccataat
     3901 gggcaaagat gttcccgttc agagccaggt aacggttgcg gttaaggcag atgctaaaac
     3961 ggcacatgtt gtgagcgtcg tggcttctcc tgacaccatc accgccgacg gcatcgatag
     4021 cagcaccatc acttcacgag tagaagatga ttacggattc ccggttgaag gtgtcgatgt
     4081 tagtcatggc ttagacacca aaggcagccc ggtagttaat attccaacta cgcgtaccga
     4141 tcagtccggg caagtcacgg cgacaataac cagtacattg gcagaaacct taacagtcaa
     4201 tgtgcaagtt cctggtacag ccaaccaatc cgcaaccatt acattggttg ccggcacggc
     4261 cgatgaaagt aagtcaattt tgaaatccga tgttggcact ctgaaggctg actaccagca
     4321 gagcgcaaaa cttacgctaa cattgcaaga caagtacggt aacccgatag tgacgtctga
     4381 tcatctggaa tttgtccagt caggcccctt cgtgaacttt ctcaagttga gcgatattga
     4441 ttacagccaa agaaattatg gcgagtacac cgtgactgtc actggcggaa aagaggggac
     4501 agcgaccctc attcccatgc tgaacggggt tcatcaggca aacttaagca tatcgctgaa
     4561 tctcatccaa tcgataaaag aaatgtccgg tcatgtcact gcaaacaacc ataccttctc
     4621 cacggctaaa ttcccgagcg aaggctttgc aggggcgtat tacacactca acaatgataa
     4681 ctttgaagcg ggtaaaaccg ttgatgatta tatgttttca agttcacagg gttgggtgtc
     4741 tgtcgatgct tcaggtaaag tttctttcgc aaatatcggc gatcaaacgt cagtcacaat
     4801 aagcgctgtt ccccgacaag gaggtacaac ctaccagacc ttaattaagc tgaaaggctg
     4861 gtgggtgaat aatggaaatc ataccaatat ctggctagct gccaatgcgc tctgtcatgc
     4921 taaaaatgat ggatataatc ttcctggcat cacacatttg acgtctggcg aaaacaaacg
     4981 cacgcaggga tcactgtatg gtgaatgggg gaacgttgga gcgttttcca gtaattcgca
     5041 atttacaccg ggagcttact ggacaagtga acctgatgat tacagtcggc actactatgt
     5101 gcagatgcta accggtatga ccggaagcga cgctgattcc agcccccaac tgaccgcctg
     5161 ccgtaaatca ctttaatttc agcagttgtt attaaaggat acccttttac gagggtatcc
     5221 tttcagactg ataacaaacg ctacattgcc ggatgtgctg cgcttatcag gcctatatag
     5281 cccctgcaat atattgaatt tattgttttt gtaccccagt cggcataaac gaccactcaa
     5341 gaaactcaac agcaaccgtt gatgtcagcc agaccccgtt ttccgcttgc cagaaaggga
     5401 tgcctcgttt cgccatctcc tgtgctttga cggttaagat aaccggcgat ccgtgtcttg
     5461 ctcccacttt gcgtgccgtt gcttcatcag cagagagatg cacgtaatga cgatcccctg
     5521 caatcagccc ctgttttttt atctcgtcaa ggaaacggct tgccgtaccg tggtagagaa
     5581 actgtggcgg tgttttttcg gcaaaggaaa tagcgacctg cgaggtcgaa tgcccctgaa
     5641 ccgcgcggat gcacctacca tcactggaat aactaaaacg ctttttatcg ctggtggcga
     5701 ccacggtatc cagcaatgca cgggttaatc gcttgcccgc tttttgggca caaagtatga
     5761 gcttatcgat atccgcccat ccttcacggt ccagtacgat accaatcgcc tccggtttgt
     5821 gtcggaggac aaaacttaaa aacttgctcg tatccgccag ctctttttcg ttgtactttg
     5881 ccatcgcgtt ttcgtccctg gattttctgt ttttatttac caatgtcatt gtcactgggt
     5941 ttatgaattt tctgcaactc aatcctgcgt agaacctttt ccacacagcg taatttttcc
     6001 gctatgcaaa aaacgcatag ccagaaacat acttgcattt tccccatacc cacatggctg
     6061 ataaggtttt tccgctccag gaattttcct ggctcaagca acacacaaac acaaaaaaac
     6121 aatggggatt gttatgacac aacaagggga tgctgtagcc ggggagctgg cgacagaaaa
     6181 agtgggcatt aaagggtatc tggctttttt tctcaccata atcttttttt ccggtgtttt
     6241 ttccggaaca gacagctggt ggcgcgtttt tgatttttcc gttctgaacg gatcgtttgg
     6301 gcaactgcct ggtgcgaacg gagccaccac ctcatttcgc ggcgcgggtg gtgcgggggc
     6361 taaagatggc tttctctttg cgctggagct ggcaccgtca gtcattctct ctctggggat
     6421 tatttcaatt accgatggtc ttggtggatt acgcgccgcg cagcagttaa tgacacctgt
     6481 gctaaaaccg ttgttgggca ttccagggat ctgctcgctg gcgctgatcg ctaacttgca
     6541 aaacactgat gccgccgccg gtatgaccaa agagctggcg caggaaggtg aaattaccga
     6601 acgcgataaa gtgattttcg ccgcatatca aaccagcggt agcgccatca tcaccaacta
     6661 cttctcttcc ggcgtcgcgg tgtttgcctt tctgggcacg tccgtaatcg tacctttagc
     6721 cgtcatcctg gtgtttaaat ttgtcggagc caacattttg cgcgtctggc ttaatttcga
     6781 agaacgtcgc aacccaacgc aaggagcgca agcatgacaa ctcaggtccg caaaaacgtc
     6841 atggatatgt ttatcgatgg cgcgcgccgt ggctttacta tcgccacgac caacctgctg
     6901 ccaaacgtgg tgatggcgtt tgtcatcatt caggcgctga aaataaccgg cctgctcgac
     6961 tgggtggggc atatctgtga acccgtaatg gcgctgtggg gattacccgg tgaagcggca
     7021 accgtccttt tagccgcatt aatgagtatg ggcggcgcag tcggtgttgc cgccagtctg
     7081 acaaccgctg gcgcattaac cggtcacgat gtcaccgttt tactgcccgc gatgtattta
     7141 atgggcaacc cggtgcaaaa tgtcggtcgc tgcctgggga cagccgaagt gaatgccaaa
     7201 tattacccgc atatcattac ggtctgcgtg attaacgcat tgctgtcgat ctgggttatg
     7261 cagcttattg tttaacaagg agttaccatg attgattata acgcagccgg ttttaccctg
     7321 ctgcagggag cgcatttgta tgcgccggaa gatcggggaa tttgcgatgt cctcgtcgct
     7381 aacggcaaaa ttatcgccgt tgccagcaat atcccttctg acattgtacc gaactgcacg
     7441 gttgtcgatc tcagtgggca gatcctctgc ccaggtttta ttgatcaaca cgtccatttg
     7501 attggcggtg gcggcgaagc aggtcccacg acgcgcacgc cggaagtggc gctaagtcgc
     7561 ctgacggaag cgggcgtcac gtcagtggtt ggtctgctgg gcaccgactc tatctctcgc
     7621 cacccggaat ccctgctcgc caagacccgt gcgctcaatg aagaaggcat cagcgcctgg
     7681 atgctgaccg gcgcttatca tgtcccttcc cgcactatta cgggttccgt ggaaaaagac
     7741 gtggcgatta tcgatcgtgt gattggcgtg aaatgcgcca tctctgatca ccgttctgcc
     7801 gcaccggacg tttatcacct ggctaatatg gcggcagaat cccgcgttgg cggtttgctc
     7861 ggcagtaaac ctggcgtcac cgtgttccac atgggcgaca gtaaaaaggc gttacagcct
     7921 atctatgacc tgctggaaaa ctgcgatgtg ccgatcagca agctgctgcc gacccacgtt
     7981 aaccgcaacg taccgttgtt tgagcaggcg ctggagttcg cgcgcaaagg cggcaccatc
     8041 gatatcacca gcagcattga cgaaccggtc gcccctgccg aaggtattgc ccgcgccgtt
     8101 caggcgggta ttccgctggc acgcgtcacc ctcagctccg acggcaacgg tagccagccg
     8161 ttcttcgatg atgaagggaa tttaacccat atcggcgttg caggttttga aacgttgctg
     8221 gaaaccgtgc aggtgctggt taaagactat gatttcagta tcagcgatgc cctgcgcccg
     8281 ctcaccagta gcgtagccgg tttccttaac ctgagcggga aaggcgaaat tctgccaggc
     8341 aatgacgcag acttactggt catgacgcca gaattgcgca ttgagcaggt atacgctcgc
     8401 ggcaaactga tggtcaaaga cggcaaagcc tgcgtgaaag gaacgtttga aacggcttaa
     8461 acggttgtca gccgccctaa tggacgtaga atgcccgcaa gggcggctga cagagtaaaa
     8521 cgtaatggat gactgtggtg cggttttgca taatattgaa accaaatggc tttatgattt
     8581 tctgaccctg gaaaaatgcc gcaatttttc ccaagcggca gtcagtcgta acgtctcgca
     8641 accggcattc agccgccgca tccgcgcgct ggaacaggcg attggcgttg aattgtttaa
     8701 ccgccaggtg acgccgctgc aactctcaga acaagggaaa atcttccatt cgcagatccg
     8761 ccatctgttg caacagttag aaagtaacct tgcagagctg cgtggcggca gcgattacgc
     8821 gcaacgtaaa atcaagatag ccgctgcaca ctctctttcc ctcggtctgt taccgtccat
     8881 tatcagccag atgccgccgc tctttacctg ggcaattgaa gccattgatg tcgatgaagc
     8941 agtcgataaa ctgcgtgaag ggcaaagtga ctgtattttt tcctttcacg acgaagattt
     9001 gctggaagcg ccgtttgacc acattcgctt atttgaatct caattgttcc ccgtttgcgc
     9061 cagtgacgaa cacggagaag cactttttaa cctcgcgcag ccacactttc cgttactgaa
     9121 ttacagccgc aactcctaca tggggcgatt gattaatcgc accctgacgc gccacagtga
     9181 gttaagtttc agcacctttt ttgtctcttc gatgagcgag cttttaaagc aggttgccct
     9241 cgacggctgt gggattgcct ggctgccgga atacgccatc caacaagaaa ttcgcagcgg
     9301 gcagctcgtt gtgctgaatc gggacgaact ggtgatcccg attcaggcct acgcataccg
     9361 gatgaatacc cgcatgaatc ccgttgccga acgcttctgg cgtgaactgc gcgagctgga
     9421 gattgtgctt agctgacatt ctcaagcgtc gcactgcgcc aaccgccatt atcccagacc
     9481 agatcaaacg ttagcggttc atcgtcgtgg tacagcatta tctcaaggct gatcaccagc
     9541 gtgtggcagt acgcttcttt caaattaacc tgcaacgccg caatccgtgg ctcatgaaat
     9601 aacagtgcag aacgcagctg acggcaatac cagctctgga gatgtttgtc gctcgggtta
     9661 tgcacttgcc actcgggcat gcctgaaaca agtaatgacg gtaatgccga atgaggagaa
     9721 ttaaaaagag tgaggagatc gctgcatacc gatttacgca gcaatgacgt tttattgcct
     9781 gaaatttcag gtaaaacagc ctgaagtgat tggcgcatca ttttgcgcac tcctgacgtt
     9841 tagcaacata tgcggcttgc cctgaacacc gggcaccact gtattgatgc tcattttcca
     9901 tagccattcc cttttgttca ttacatccat gtcagtctga gacaggcctg aatcctgtca
     9961 gcaaagtata aaaccttact tgcctatgaa tatctactga cttttcagga aaaaagtcat
    10021 gatttgataa aaatcatcaa actcaaaaaa
//
LOCUS       DAZKFU020000176         9868 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZKFU020000176 DAZKFU020000000
VERSION     DAZKFU020000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.178"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..147)
                     /locus_tag="ACHOQQ_RS17510"
                     /old_locus_tag="pgaptmp_003502"
     CDS             complement(<1..147)
                     /locus_tag="ACHOQQ_RS17510"
                     /old_locus_tag="pgaptmp_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471711.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKISKMMNGKLIYSWLKWQCG
                     EFFFL"
     gene            225..1319
                     /gene="ddlA"
                     /locus_tag="ACHOQQ_RS17515"
                     /old_locus_tag="pgaptmp_003503"
     CDS             225..1319
                     /gene="ddlA"
                     /locus_tag="ACHOQQ_RS17515"
                     /old_locus_tag="pgaptmp_003503"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4471712.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1343..1555)
                     /gene="yaiZ"
                     /locus_tag="ACHOQQ_RS17520"
                     /old_locus_tag="pgaptmp_003504"
     CDS             complement(1343..1555)
                     /gene="yaiZ"
                     /locus_tag="ACHOQQ_RS17520"
                     /old_locus_tag="pgaptmp_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGU4471713.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1815..2123
                     /gene="yaiY"
                     /locus_tag="ACHOQQ_RS17525"
                     /old_locus_tag="pgaptmp_003505"
     CDS             1815..2123
                     /gene="yaiY"
                     /locus_tag="ACHOQQ_RS17525"
                     /old_locus_tag="pgaptmp_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGU4471714.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2182..3276)
                     /gene="yaiW"
                     /locus_tag="ACHOQQ_RS17530"
                     /old_locus_tag="pgaptmp_003506"
     CDS             complement(2182..3276)
                     /gene="yaiW"
                     /locus_tag="ACHOQQ_RS17530"
                     /old_locus_tag="pgaptmp_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGU4471715.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSRAPQPLKKGEKEIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3289..4509)
                     /gene="sbmA"
                     /locus_tag="ACHOQQ_RS17535"
                     /old_locus_tag="pgaptmp_003507"
     CDS             complement(3289..4509)
                     /gene="sbmA"
                     /locus_tag="ACHOQQ_RS17535"
                     /old_locus_tag="pgaptmp_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGU4471716.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4861..6018
                     /gene="ampH"
                     /locus_tag="ACHOQQ_RS17540"
                     /old_locus_tag="pgaptmp_003508"
     CDS             4861..6018
                     /gene="ampH"
                     /locus_tag="ACHOQQ_RS17540"
                     /old_locus_tag="pgaptmp_003508"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGU4471717.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6019..6642)
                     /gene="iprA"
                     /locus_tag="ACHOQQ_RS17545"
                     /old_locus_tag="pgaptmp_003509"
     CDS             complement(6019..6642)
                     /gene="iprA"
                     /locus_tag="ACHOQQ_RS17545"
                     /old_locus_tag="pgaptmp_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HGU4471718.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6730..9627)
                     /locus_tag="ACHOQQ_RS17550"
                     /old_locus_tag="pgaptmp_003510"
     CDS             complement(6730..9627)
                     /locus_tag="ACHOQQ_RS17550"
                     /old_locus_tag="pgaptmp_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4471719.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYVDTNKPIYN
                     IDTDYLTYSGYVYNTVAKDSGYYDTVFSGDTVNGVISTYYLNYDDYAAGTANTVSINN
                     STVHGMITSKYVPNFGDAAKAHDWIDGDTFTLNISNSTIDDDYEGFYFTDTYLDADGK
                     TVKSDNYEYSVAGMGTAVTLDVESNINISNNSHVAGITLEQNDTTNTAYKTGATQWDN
                     TITVNDSTVTSGSWTEDEQQDGHFGNSAEPSDYSGDGRVGYHSNDNALEFIDDSSSDY
                     RMVNNVTFNNSQLMGDVYFQSTWNNNFDYIGHPTDTDSNVYTHGGWIDDDQNVDHLTL
                     TLNHTKWVGGAYIDYAGDDIVTPDHFYDVAANSLDPNATNYDGWNRMDSAASFQSGVF
                     DVVLNDGSEWDVAKDSRIDTLAVNNSQVDVANGVELTSDTIGLNDGSVLNIGEGGQVN
                     TDHLTVNTFSTVTLANDISSDWALDALYANTITVTNGGVLDVQTSNTDAANVFTTDTL
                     ELTSGNVKDNHGNVYAGVFDINSNDYILNADLVNDRTNDTSKANYGYGVIAMNSDGHL
                     TVNGNGDINNVDTAATNQGGEADNSTADNVVAATGNYKVRIDNSTGAGAIADYAGKQL
                     IYIDDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNI
                     WNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKI
                     DGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFN
                     NDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQL
                     SNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSI
                     DNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2556 a         2439 c         2339 g         2534 t
ORIGIN      
        1 taagaaaaaa aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat
       61 catttttgag atcttacgca catctttgag ataacgcact ttcttcacca gtacgttgca
      121 cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
      181 gtggtaatgt atgcacttta tgttagacgg gataggtttt taagatggaa aaactgcggg
      241 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
      301 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
      361 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
      421 atattgcgtt gcgcccttcg gcgaccagcc tcgcccaggt gccaggtaaa catgagcatc
      481 agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg
      541 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
      601 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
      661 gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc
      721 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
      781 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
      841 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag
      901 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
      961 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
     1021 cgaaagtggt agttccggca gccattgcgc cagaaatcaa cgataagatc cgggcgattg
     1081 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
     1141 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
     1201 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
     1261 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
     1321 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
     1381 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
     1441 gccaaccttt tgcttcaaat cccagtaacc ccaccacgcc attaagaatg aatataaggc
     1501 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggctg
     1561 ctccgttaac gaaaaacaac atccttgcat attctgcaac aaccgtctgt ggtgtagaca
     1621 acattttcgc gatagttaat aaatttaact taaagttgct attttgtgac tcgcaacgaa
     1681 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
     1741 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
     1801 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
     1861 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
     1921 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
     1981 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
     2041 tcgggctgat cccgttttta gtaggctgcc tcatttttgc agtagtggcg ctatggctgc
     2101 actggcgaca tcgccgccag taagatttac gttgccagat acggcctaaa acgccttatc
     2161 tggcatgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
     2221 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
     2281 aggcaacatt tcgcggggga gtgatttacc cgttttcgct tcggcaagtt gataaacttt
     2341 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctagct gacgacgaat
     2401 ttcgctgtcg ctcatcccca gttttgcagc cagtttgcgc gttgccagtt cggttttccc
     2461 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
     2521 cttactgacc gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
     2581 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
     2641 aaaccacaac ccgccgcggc gactgaagac ttcctgacgg actgtaccgt ccattttcca
     2701 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
     2761 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggcaccatgc tgatcagatc
     2821 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
     2881 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaaggag gaatgtgcat
     2941 ccgttcagca cgacggtcaa tctcttgcca ggcgattttg cttaagcccg gaacggccgg
     3001 atctgcctga taatttgact cctgctgcgc caccgccagc accgagcaga cgttttccag
     3061 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
     3121 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gccacatcta tctctttttc
     3181 gccctttttc aacggttgcg gagcccgact actacacccc gctaaaacca gaaccgccaa
     3241 caatgacagc gaagatagag gattgacgcg cgacattact tctccttttt agctcaaggt
     3301 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
     3361 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
     3421 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
     3481 aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat
     3541 gcgagcgatg ttgaaataca tatagtgaaa atagaggcgg aaatagtttt tccgtacggc
     3601 gctaaacagc tcgcgtaccg ttggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
     3661 cagctcttta cggtaggcag cctctactcg ctggttttta aactccagcc ccggcagttt
     3721 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
     3781 cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac
     3841 cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga cacccatatt
     3901 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg cgccttcgat
     3961 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
     4021 cacgtagtga ctgacaaaga agttattcag cacactgatc actaccgcga tcagcgcaat
     4081 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
     4141 tagcgcggtt tgaatcagat catagaacgg tgcataccag gcgttgacgg caaccccgac
     4201 ttccaccaaa aaccaggtga cgaagatgat cagtgcagta ccgagtattg accagtattg
     4261 ccaacggtgc gggctgtaga taaaccagaa caatgcaaaa agtcctacgc aaacaatgta
     4321 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
     4381 cccggaagcg ccggtgatac gcgccaccca gtcaccccca ccggcttgcc agaagataac
     4441 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga
     4501 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
     4561 catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
     4621 ttagttcgcc agcctggcca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
     4681 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
     4741 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
     4801 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatttatg gacaccaccg
     4861 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
     4921 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
     4981 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
     5041 cgcgtctttc gcagttatgg cgaaacgcga cctggtaata acgttcgccc gcagctggat
     5101 tcggtcgtgc gtattgcttc cctcaccaag ctaatgacca gtgaaatgct ggtgaaattg
     5161 ctcgaccagg ggaccgtgaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
     5221 cgcgtgccaa cctacaatgg gacgcccatt acgctggtca atctggcaac ccataccagc
     5281 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
     5341 cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag
     5401 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
     5461 aagccttata cccagttgtt tgaagagcag attacccgtc cattggggat gaaagacacc
     5521 acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgcagagcg cggtgccagt
     5581 ccatgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
     5641 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
     5701 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
     5761 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
     5821 cgtccgggga ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg
     5881 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
     5941 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
     6001 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
     6061 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
     6121 acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc
     6181 ttcattccag tcaatcattg ataataatgt tgcgcggatt tgttcgtagg aattatgccc
     6241 aatgagctgc acgtcgcgta attccagaat tctattttgc caggctaacc agtaaaaagc
     6301 gtctcgccag agttgatttt gttcaataag cgtaatggtt tgtttggctg gtagatgata
     6361 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
     6421 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
     6481 cagagagata acgccctcta aaataacgaa agtatcttcg ttattgacat cactggaaaa
     6541 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
     6601 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
     6661 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagaat ttcttcggag
     6721 tgaagaatat taccaggtat atttaacacc cacgttcgcg gaccaatctt gatctacgtc
     6781 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
     6841 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt
     6901 cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag
     6961 agcctggtct tcgctgtaag tgaaggtata acctgcatct acacccagtt cataacgcat
     7021 gctgtcgtaa gactgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga
     7081 ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa
     7141 gtcgtaaccg gctttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta
     7201 agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaagctacc
     7261 atcaacaaag acgttgttcg cgaagtgagc agaagagtag atgtaggcag tctggctgtc
     7321 ttgatccacc tgaccagaac ggtcattcat gtcaccttta gcgaagcctg cagccgcacc
     7381 gacgatccac ttagcgttgt taccgtcaat tttggtatca acaccgacca tgatgccgtt
     7441 aacatcctga tcatagttga tggtgccgtt gtcaccgttg aagttaccac cgaagtagct
     7501 tacccatgcg ccgccgttat cagccaggcc atgacgagag ttggtcaaac gagtaccaac
     7561 ggtgtcttgt tccaggttcc agatattggt gttcgcagac gggatgctca gcgccatgtt
     7621 agcgtagtca gtcagctcca tctgttgcag aacaacggtg ttaccgcgct gttcagcctg
     7681 ataggtgtat gcacccaggt cagctttgtt agcagcagag aaggtcgcgt tggttttggt
     7741 atcatcgata tagatgagct gcttacctgc gtaatcagca atcgcaccag cgccagtgga
     7801 gttgtcgata cgaactttgt agttaccggt tgcagctaca acgttgtctg cagtgctgtt
     7861 atcggcttca ccaccctggt tggttgcagc ggtatcaacg ttgttgatat caccattacc
     7921 attaacagtc aggtgaccgt cagagttcat tgcgattaca ccgtaaccgt agttagcttt
     7981 gctggtgtcg ttggtacgat cgttgaccag atccgcattc aagatgtagt cgttgctgtt
     8041 gatatcgaac acaccagcat aaacattacc atggttgtct ttcacgttac cgctggtcag
     8101 ttccagagta tcggtggtga agacgtttgc agcgtcagta ttactggtct gcacatccaa
     8161 tacaccgccg ttagttacgg tgatggtgtt ggcgtacagt gcgtcgagtg cccaatcgct
     8221 gctaatatca ttagctaagg tgacggtact gaaggtgtta acagtcaagt gatctgtatt
     8281 aacctgacca ccttcaccga tgttcaaaac agaaccgtca ttcaggccga tagtgtcaga
     8341 cgtcagctca acaccgttag caacatcaac ctgagagttg tttacagcca gggtatcaat
     8401 gcgagagtct ttcgccacat cccattctga accatcgttc agaacaacat caaacacgcc
     8461 gctctggaaa gaagctgcgc tgtccatacg gttccaacca tcatagttag tagcgtttgg
     8521 atccaagctg ttagccgcaa cgtcatagaa atgatccgga gttacgatgt catcaccagc
     8581 ataatcaata tacgcaccac cgacccattt agtatggttc agggtcaggg tcagatgatc
     8641 gacgttctga tcatcatcaa tccatccacc gtgggtatag acgttggaat ctgtatcagt
     8701 tggatgaccg atgtaatcaa agttattatt ccaggtactc tggaaataaa catcacccat
     8761 caactgagag ttgttgaagg taacattgtt gaccatgcgg taatcagaag aactgtcatc
     8821 aatgaactct aaagcgttat cattgctgtg gtaaccgaca cggccatcac cagagtaatc
     8881 actcggctca gcagaattac caaagtgacc atcctgctgc tcgtcttcgg tccaagaacc
     8941 agaagttaca gttgagtcat taacagtgat ggtgttgtcc cactgagttg cgccagtttt
     9001 gtacgcagtg ttagtggtat cgttctgttc cagcgtaata cctgcaacat gagagttgtt
     9061 gctgatatta atgttgcttt caacatccag cgttactgcg gtacccatgc cagcaactga
     9121 atattcatag ttgtctgact taacagtttt accatcagca tccaaatagg tgtcggtgaa
     9181 gtaaaaacct tcataatcat catcaatcgt tgaattgctg atgttcagag tgaaggtgtc
     9241 accgtcaatc cagtcgtgag cttttgcagc gtcgccgaag ttaggaacat attttgacgt
     9301 gatcataccg tgaacagttg agttattaat actcacagta tttgcagtac ctgctgcata
     9361 gtcatcatag ttcaggtaat aggtagaaat tacaccattt acagtgtcac ctgagaaaac
     9421 ggtgtcatag taaccgctat ctttagctac tgtgttataa acataaccac tgtaggtcag
     9481 gtaatcggta tcaatgttgt aaattggttt gttggtatca acataaccaa aagtattata
     9541 agtagcagca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta attgtgatac
     9601 tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt aataagttga
     9661 tccatcaatt atcaatgcat agaaaacatc ttatgtttcg cgtttacgat tatgcggcgt
     9721 tatagaggaa tggttcaatt attctttccc ctaagtccga ttgcggacat atataaaaat
     9781 atctatacgc acaaagaata agttgacaaa aacactgtat ttcaacacgt taatgaaatc
     9841 attaattttg aattatagat cttgaaaa
//
LOCUS       DAZKFU020000177         9815 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZKFU020000177 DAZKFU020000000
VERSION     DAZKFU020000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.179"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            86..1063
                     /gene="glaH"
                     /locus_tag="ACHOQQ_RS17555"
                     /old_locus_tag="pgaptmp_003511"
     CDS             86..1063
                     /gene="glaH"
                     /locus_tag="ACHOQQ_RS17555"
                     /old_locus_tag="pgaptmp_003511"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGU4471720.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLTPSAQSPRLLELTFTEQTTKQ
                     FLAQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1082..2350
                     /gene="lhgO"
                     /locus_tag="ACHOQQ_RS17560"
                     /old_locus_tag="pgaptmp_003512"
     CDS             1082..2350
                     /gene="lhgO"
                     /locus_tag="ACHOQQ_RS17560"
                     /old_locus_tag="pgaptmp_003512"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGU4471721.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARNVDALHAAFNQ"
     gene            2373..3821
                     /gene="gabD"
                     /locus_tag="ACHOQQ_RS17565"
                     /old_locus_tag="pgaptmp_003513"
     CDS             2373..3821
                     /gene="gabD"
                     /locus_tag="ACHOQQ_RS17565"
                     /old_locus_tag="pgaptmp_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4471722.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGD
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3835..5115
                     /gene="gabT"
                     /locus_tag="ACHOQQ_RS17570"
                     /old_locus_tag="pgaptmp_003514"
     CDS             3835..5115
                     /gene="gabT"
                     /locus_tag="ACHOQQ_RS17570"
                     /old_locus_tag="pgaptmp_003514"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGU4471723.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHTEIGDVRGLGAMIAIELFEDGDHSKPNAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFAEAKQ"
     gene            5533..6933
                     /gene="gabP"
                     /locus_tag="ACHOQQ_RS17575"
                     /old_locus_tag="pgaptmp_003515"
     CDS             5533..6933
                     /gene="gabP"
                     /locus_tag="ACHOQQ_RS17575"
                     /old_locus_tag="pgaptmp_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGU4471724.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6954..7616
                     /gene="csiR"
                     /locus_tag="ACHOQQ_RS17580"
                     /old_locus_tag="pgaptmp_003516"
     CDS             6954..7616
                     /gene="csiR"
                     /locus_tag="ACHOQQ_RS17580"
                     /old_locus_tag="pgaptmp_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGU4471725.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMNLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEARDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRQTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMCQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7617..8066)
                     /gene="kbp"
                     /locus_tag="ACHOQQ_RS17585"
                     /old_locus_tag="pgaptmp_003517"
     CDS             complement(7617..8066)
                     /gene="kbp"
                     /locus_tag="ACHOQQ_RS17585"
                     /old_locus_tag="pgaptmp_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGU4471726.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8150..8308)
                     /locus_tag="ACHOQQ_RS17590"
                     /old_locus_tag="pgaptmp_003518"
     CDS             complement(8150..8308)
                     /locus_tag="ACHOQQ_RS17590"
                     /old_locus_tag="pgaptmp_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU4471727.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8491..8790
                     /locus_tag="ACHOQQ_RS17595"
                     /old_locus_tag="pgaptmp_003519"
     CDS             8491..8790
                     /locus_tag="ACHOQQ_RS17595"
                     /old_locus_tag="pgaptmp_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4471728.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8800..9318
                     /gene="ygaP"
                     /locus_tag="ACHOQQ_RS17600"
                     /old_locus_tag="pgaptmp_003520"
     CDS             8800..9318
                     /gene="ygaP"
                     /locus_tag="ACHOQQ_RS17600"
                     /old_locus_tag="pgaptmp_003520"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGU4471729.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(9371..9775)
                     /gene="stpA"
                     /locus_tag="ACHOQQ_RS17605"
                     /old_locus_tag="pgaptmp_003521"
     CDS             complement(9371..9775)
                     /gene="stpA"
                     /locus_tag="ACHOQQ_RS17605"
                     /old_locus_tag="pgaptmp_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGU4471730.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2215 a         2570 c         2739 g         2291 t
ORIGIN      
        1 ttttcagaaa tgtagatatt tttagattat ggctacgaaa tgagcatcac gcactctccc
       61 tgaaacctca agaggatcgc ttctgatgaa tgcactgacc gccgtacaaa acaacgctgt
      121 cgattcaggc caggactata gcggattcac cctcacccca tcggcgcaat ccccgcgtct
      181 gctggaactc acctttaccg aacagacgac caaacagttc ctcgcgcagg ttgccgagtg
      241 gcccgtgcag gcgctggagt acaaatcgtt tctgcgtttt cgggtaggca aaattcttga
      301 tgatctgtgt gcgaatcagc tgcaaccgtt gctgttgaag accctgctaa accgcgctga
      361 aggtgcgttg ttgatcaatg cggtgggggt cgatgatgtg aagcaagcgg atgagatggt
      421 gaagctggca acggcggtgg cgcatctgat tggtcgctcc aatttcgacg cgatgagcgg
      481 tcagtattac gcgcgttttg tagtgaaaaa tgtcgataac tcagacagct atctgcgtca
      541 gccgcaccgc gtgatggagc tgcacaacga cggcacctac gtggaagaga tcaccgacta
      601 tgtgctgatg atgaaaatcg acgagcaaaa catgcagggc ggaaattcgt tgctgctgca
      661 tctcgatgac tgggaacatc tcgaccacta tttccgccac ccgctggcgc gccgcccaat
      721 gcgctttgcc gcgccgccga gtaaaaacgt cagcaaagat gttttccatc cggtgttcga
      781 cgttgatcaa cagggtcgcc cggtgatgcg ttatatcgac caattcgtcc agccaaaaga
      841 ttttgaagaa ggcgtgtggt tgagcgagct ttccgatgcc attgaaacca gcaaaggcat
      901 tctttctgtg cccgttcccg ttggcaaatt cctgttgatt aacaacctgt tctggctgca
      961 cggtcgcgac cgctttactc cgcacccgga tctgcgccgt gaactgatgc gtcagcgtgg
     1021 ctatttcgct tacgccactc accactacca gacgcatcag tagcgcaaag gaattgagcg
     1081 gatgtatgat tttgtgatta ttggcggtgg catcatcggc atgtcgaccg ccatgcaact
     1141 gattgatgtc tacccggacg cccgcattgc gttgctggaa aaagagtccg gcccggcctg
     1201 tcaccagacg gggcacaaca gcggcgtgat ccatgccggg gtctattaca cgcccggcag
     1261 cctgaaggca cagttttgcc tggcggggaa ccgcgccacc aaagcctttt gcgatcaaaa
     1321 cggcattcgc tacgacaact gcggcaagat gctggtcgcc acgtccgatc tcgaaatgga
     1381 acggatgcgc gcgttgtggg aacggacggc ggcgaacggc atcgagcgcg agtggttaaa
     1441 cgccgatgaa ctgcgcgagc gcgaaccgaa tatcaccggg cttggcggta tttttgtgcc
     1501 gtccagcggc attgtcagct accgcgaagt cacggcggcg atggcaaaaa tcttccaggc
     1561 cagaggcggc gagattatct ataacgccga agtcagcggc cttagtgagc ataaaaacgg
     1621 cgtggtgata cgtacccgtc agggtggcga atacgaagcg tcaacgctga ttagctgttc
     1681 cgggctgatg gctgaccggc tggtgaaaat gctcggcctc gaaccgggct ttattatctg
     1741 cccgttccgt ggcgagtatt tccgcctggc gccggagcat aaccagattg ttaaccacct
     1801 gatttacccc atccccgacc cggcaatgcc gtttttaggc gttcatctca cccgcatgat
     1861 cgacggtagc gtcaccgttg ggccaaacgc agtgctggcg ttcaaacgcg aaggctatcg
     1921 caagcgcgac ttctcgttta gcgacacgct ggagatttta ggctcgtcgg ggattcgccg
     1981 ggtgctgcaa aaccatctac gctcaggact gggcgagatg aaaaactcgc tgtgcaaaag
     2041 cggctatctg cggctggtgc aaaagtattg tccccggctt tcgttaagcg atctccagcc
     2101 ctggcccgcc ggtgtgcggg cgcaggcggt atcgccggac ggcaagctga ttgacgattt
     2161 tctgtttgtc accaccccgc gcacgatcca cacctgcaat gcgccctccc cggcagcgac
     2221 atcagcaatt cctattggtg cgcatattgt cagcaaggta caaacgctgt tggcaagcca
     2281 gagtaacccc ggacgcacgc tgcgagcggc acgtaatgtg gatgccttac acgccgcatt
     2341 taatcaataa cctttgaaaa caggatgtag cgatgaaact gaacgacagc aacttattcc
     2401 gccaacaggc gttgattaac ggggaatggc tggacgccaa caatggtgaa gtcatcgacg
     2461 tcaccaatcc ggcgaacggc gacaagctgg gtagcgtgcc gaaaatgggc gcagatgaaa
     2521 cccgcgccgc tatcgacgcc gccaaccgcg ccctgcccgc atggcgcgcg ctcaccgcca
     2581 aagaacgcgc caacattctg cgcaactggt tcaatttgat gatggagcat caggacgatt
     2641 tagcgcgcct gatgaccctc gaacagggta aaccgctggc tgaagccaaa ggcgaaatca
     2701 gctacgccgc ctcttttatt gagtggtttg ccgaagaagg caaacgcatt tatggcgaca
     2761 ccattcccgg tcatcaggcc gataagcgcc tgattgtgat caagcagccg attggcgtta
     2821 ccgcggctat cacgccgtgg aacttcccgg cggcgatgat tacccgtaaa gccggcccgg
     2881 cgctggcggc aggctgcacc atggtactga agcccgccag tcagacgccg ttctctgcgc
     2941 tggcgctggc ggagctggcg atccgcgcgg gcattccggc tggcgtgttt aacgtggtca
     3001 ccggttcggc gggcgcggtg ggtgacgaac tgaccagcaa cccgctggta cgcaaactgt
     3061 cgtttaccgg ttcgaccgaa attggccgcc agttaatgga acagtgcgcg aaagacatca
     3121 aaaaagtgtc gctggagctg ggcggcaacg cgccgtttat cgtctttgac gatgccgatc
     3181 tcgataaagc cgtggaaggc gcactggcct cgaaattccg caacgccggg caaacctgcg
     3241 tctgcgccaa ccgtttatac gtgcaggacg gcgtgtatga ccgctttgcc gaaaaattgc
     3301 agcaggcggt gagcaaactg catatcggcg acgggctgga taaaggcgtc accatcgggc
     3361 cgctgatcga tgaaaaagcg gtagcaaaag tggaagagca tattgccgat gcgctggaaa
     3421 aaggcgcgcg cgtagtttgc ggtggtaaag cacacgaacg cggcggtaac ttcttccagc
     3481 cgaccattct ggtggacgtt ccagccaacg ccaaagtgtc gaaagaagag acgttcggcc
     3541 ccctcgcccc gctgttccgt tttaaagatg aagccgatgt gatcgcgcag gccaatgaca
     3601 ccgagtttgg ccttgccgcc tatttctacg cccgtgattt aagtcgcgtc ttccgcgtgg
     3661 gcgaggcgct ggagtacggc atcgtcggca tcaataccgg gattatttcc aatgaagtgg
     3721 ccccgttcgg cggcattaaa gcctcgggtc tgggtcgtga aggttcgaag tatggcatcg
     3781 aagattactt agaaatcaaa tatatgtgca tcggtcttta actggagaac gcgaatgagc
     3841 agcaataaag agttaatgca gcgccgcagt caggcagttc ctcgtggcgt tgggcaaatt
     3901 caccccattt tcgctgatcg cgcggaaaac tgccgggtgt gggacgttga aggccgtgag
     3961 tatcttgatt tcgcgggcgg cattgcggtg ctcaataccg ggcacctgca tccgaaagtg
     4021 gttgcggcgg tggaagcgca gttgaaaaaa ttgtcgcaca cctgcttcca ggtgctggct
     4081 tacgagccgt atctggagct gtgcgagatt atgaatcaga aggtgccggg cgattttgcc
     4141 aagaaaacgc tgctggtcac cacgggttcc gaagcggtgg aaaacgcggt gaaaatcgcc
     4201 cgcgctgcca ccaaacgtag cggcaccatc gcttttagcg gcgcgtatca cgggcgcacg
     4261 cactacacgc tggcgctgac cggcaaggtg aatccgtact ctgcgggcat gggcctgatg
     4321 ccagggcacg tttatcgcgc gctttatcct tgcccgctgc acggcatcag tgaagatgac
     4381 gctatcgcca gcatccaccg gatcttcaaa aatgacgccg cgccggaaga tatcgccgcc
     4441 atcgtgattg agccggttca gggcgaaggc ggtttctacg ccgcgacgcc tgcgtttatg
     4501 cagcgtttac gcgcgctgtg tgacgagcac gggatcatgc tgattgccga tgaagtgcag
     4561 agcggcgcgg ggcgtaccgg cacgctgttt gcgatggagc aaatgggcgt ggcaccggat
     4621 ctcaccacct ttgcgaaatc gatcgcaggc ggtttcccgc tggcgggcgt caccgggcgc
     4681 gccgaagtga tggatgccgt cgctccaggc gggctgggcg gcacctatgc gggtaacccg
     4741 attgcctgcg tggctgcgct ggaagtgttg aaggtgtttg agcaggaaaa tctgctgcaa
     4801 aaagccaacg atctggggca gaagttgaaa gacggattgc tggcgatcgc cgaaaaacac
     4861 accgagatcg gcgacgtacg cgggctgggg gcgatgatcg ccatcgagct gtttgaagac
     4921 ggcgatcaca gtaaaccgaa cgccaaactc accgccgaga tcgtggcgcg cgcccgcgat
     4981 aagggcctga ttcttctctc ctgcggcccg tattacaacg tgctgcgcat ccttgtaccg
     5041 ctcacaattg aagatgctca gattcgtcag ggtctggaga tcatcggcca gtgttttgct
     5101 gaggcgaagc agtagcaccg cccccatgcc ggaggcgacg ctgcgcgtct tgtccggcct
     5161 acgagagtgt aggtcggata aggcgtttac gccgcatccg acaatcggcg caccatgccg
     5221 gaggcgacgc ttacgcgtct tgtccggcct acggaaatgt aggtcggata aggcgtttgc
     5281 gccgcatccg acaatcggcg cacccatgcc ggaggcgacg ctttgcgtct tgtccggcct
     5341 acggggattc tcgtcggata aggcgtttac gccgcatccg acaatctgta cgtgaacagg
     5401 aagaaatcta tgttggccgg gtaaggcgga gccgctctcc ggcaaaaaga atcaataaca
     5461 attaaacgcg tgacccgacg cgggaaatgt cggggcgctc tcccaagtga cacactttcg
     5521 agaggattca ggatggggca atcatcgcaa ccacatgagt taggcggcgg gctgaagtca
     5581 cgccacgtca ccatgttgtc tattgccggt gttatcggcg caagtctgtt tgtcggttcc
     5641 agcgtcgcca tcgccgaagc gggcccggcg gtattactgg cctatctgtt cgccggatta
     5701 ctggtggtta tgattatgcg gatgttggcg gaaatggcgg ttgccacgcc cgataccggt
     5761 tcgttttcca cctatgccga taaagccatt ggccgctggg cgggctatac catcggctgg
     5821 ctgtactggt ggttttgggt actggttatc ccactggaag ccaacatcgc cgccatgatc
     5881 ctgcactcgt gggttccagg cattcccatc tggttatttt ccctcgtcat taccctcgcc
     5941 ttaactggca gtaatttatt aagcgttaaa aactacggcg aatttgagtt ctggctggcg
     6001 ctgtgcaaag tcatcgctat cctggccttt attttccttg gtgcagtcgc aattagcggt
     6061 ttttacccgt atgccgacgt gagcgggatc tcaagattgt gggatagcgg cggctttatg
     6121 cccaacggtt tcggtgcggt attaagtgcg atgttgatca ccatgttctc gtttatgggc
     6181 gcagaaattg tcaccattgc cgccgcggaa tccgacacgc cggaaaaaca tattgtccgc
     6241 gccactaact cggttatctg gcgtatttct atcttctatt tgtgctctat ttttgtcgtc
     6301 gtggcattaa taccgtggaa tatgccgggg ctgaaagccg ttggttctta tcgctcggtt
     6361 ctggaattgc tcaatattcc ccatgcgaaa ttaatcatgg actgcgtgat attactttcc
     6421 gtaaccagtt gcctgaactc ggcgctgtat accgcttcca ggatgctcta ctccttaagc
     6481 cgtcgcggtg atgcgcccgc ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg
     6541 gcggtgttac tctccaccgg cgcggcattt ttaacggtgg tggtgaacta ttacgcacct
     6601 gcgaaagtgt ttaaattcct gatcgacagc tccggcgcta tcgccctgct ggtttattta
     6661 gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt
     6721 cgcctgcgca tgtggcttta cccgtggctc acctggctgg tcatcggctt tattaccttt
     6781 gtgttggtgg tgatgctatt ccgcccggcg caacagttag aagtgatctc cactggctta
     6841 ttagcgatag ggattatctg taccgtgcca attatggctc gctggaaaaa gctggtattg
     6901 tggcaaaaaa cacccgttca taatacgcgc tgataatgat caggagtcac accatgacca
     6961 ttacgtctct ggatggctat cgctggctga agaacgatat tattcgcggt aattttcaac
     7021 cggatgaaaa attacgaatg aatttgctga catcgcgtta tgcacttggc gttgggccgt
     7081 tacgggaagc tctttcgcaa ctggtggcgg aacggctggt cacggtggtg aatcaaaaag
     7141 ggtatcgggt ggcgtctatg tcagagcagg agctgctcga tattttcgac gcccgcgcca
     7201 atatggaagc gatgttagtg agtctggcga ttgcccgcgg tggcgatgag tgggaggcag
     7261 acgttctcgc aaaagcgcat ctgttgagta agcttgaggc ccgtgacgcc agcgagaaaa
     7321 tgcttgatga gtgggatctg cgtcatcagg cgtttcatac ggcaattgtg gcgggctgtg
     7381 gttctcacta tttgctgcaa atgcgtgaac ggttgtttga tctggcggcg cgctatcgat
     7441 ttatctggct gcgacaaaca gtgctttcgg tggaaatgct ggaggataaa cacgatcagc
     7501 accagaccct gactgcggcg gtactggcgc gagataccgc gcgcgccagt gagttaatgt
     7561 gccagcattt actgacgcca attcccatta tccagcaggc gatggctggc aattaactac
     7621 tcttcaggaa tacgcaacac ttgccccggg taaattttat ccgggctttt tagcatcggt
     7681 ttattcgctt cgaagatttt attgtacaga ttggcgttac cgtagacctg tttggaaatg
     7741 gcactcagag tgtcgccaga cttaacggta taaaactggc tggcagtggc tggtgtcgcc
     7801 gttttcacct gatcatcgac actggcaata ccggaaatat tccccaccgc aacaaggatt
     7861 ttctccttcg cctcctgact caggccgtca ccagtgaccg tcgctttgcc gtcggcaatt
     7921 tgaatgttca ctttatcggc atcaggtata ccggttttgc tcagatgctc ctgcaccttc
     7981 ttcgcctgat cgtctttatc gtgctgacct gtaaccgcgt cccagagttt ttctccggca
     8041 tctttcacaa aattgaacag ccccatagct acctcattcg ttaacggaaa cactcagaaa
     8101 gtgtagcaga ggaacgcggc gcgttaacgc cgcggggatg agcacaggtt taatcgcggg
     8161 tttgcaccca gaacgcgtga atcaaaccgg ggatataccc aagcagtgtc aacagaatat
     8221 taataatgaa cgcccaaccg aaccctttac cgagcagcac gccgagcggc ggcagaatga
     8281 tggtgatgac gattctccag aaacccatat gtactcccta taagaaaatt actcattgtt
     8341 taaaaagaga ttttatctct taagcgtagt aattttagcg gaggctgcca gtttcagctt
     8401 acgcttttac tctcatttac gctgctgatg ctggtatatg ttgcattttt agtttagagt
     8461 ttacttaatt tagctaacac ttaaatgact atgactgaac tcgcacaatt acaggccagt
     8521 gccgaacagg cagcggcctt attgaaagca atgagccacc ctaaacggtt gctgattctg
     8581 tgcatgctta gcggttcacc cggcaccagc gcgggagagc tgacgcgcat taccggactg
     8641 agtgcctctg cgacatcaca gcatctcgcc cgtatgcggg acgaagggct tatcgacagc
     8701 caacgggatg cccaacgcat tctatattcc attaaaaatg aggcggtaaa tgccattatc
     8761 gccaccctga aaaatgtcta ttgtccgtaa ggagtcacta tggctttgac aaccatttcg
     8821 ccgcatgatg cacaagaatt aatcgcacgc ggcgcaaagt taatcgatat tcgtgacgct
     8881 gatgaatatc ttcgtgaaca tattcctgaa gcagatctgg ctccattatc cgtgctggaa
     8941 cagtcaggtc tcccggctaa attacgtcgc gagcaaatta tattccactg ccaggcaggt
     9001 aaacgcacca gtaataacgc cgataaatta gcagcgattg ccgcccccgc agaaatcttt
     9061 ttactcgaag atgggattga tggctggaaa aaagcaggat tgccagtagc ggtaaataaa
     9121 tctcaaccct tgccgttaat gcgccaggtg cagatcgctg cgggtggttt aatattaatc
     9181 ggcgttgtac tgggttatac cgtaaatagc ggtttcttct tattaagcgg ctttgttggt
     9241 gccgggttac tgtttgcagg aattagcggt ttttgtggga tggcaaggtt gttagataag
     9301 atgccgtgga accaatgagc ttgagaagcg acgccggacg cgccatagca gcgacatccg
     9361 gcctcagtaa ttagatcagg aaatcgtcga gagatttacc ttctgccagc gcctgagcga
     9421 ttggcttcgg tgtacggccc tgaccggtcc aggttttagt ttcaccgtta acatcggtga
     9481 atttatattt cgccggacgc ggctggcgtt ttttaccagc acgtggtgca gcagcagagc
     9541 tattacccaa taactcttcc gggttaattc cgtcagcttt catcagctcc agccaggtgc
     9601 taattttttc ctggcgctct gccagttcac gctgctgctg ttcttcttct tcacgtcttt
     9661 ctttagtgac aaccctgaat ttttcgagca tttcttcaag aacgtcaatg gagaattcgc
     9721 gagccatcgc acggagggtg cgaatgttat ttaaactttg taacattacg gacataataa
     9781 agaaaacctt ttaacgccaa aacaaaaaag tattt
//
LOCUS       DAZKFU020000178         9810 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZKFU020000178 DAZKFU020000000
VERSION     DAZKFU020000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.180"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(91..2664)
                     /gene="clpB"
                     /locus_tag="ACHOQQ_RS17610"
                     /old_locus_tag="pgaptmp_003522"
     CDS             complement(91..2664)
                     /gene="clpB"
                     /locus_tag="ACHOQQ_RS17610"
                     /old_locus_tag="pgaptmp_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGU4471731.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2794..3525)
                     /gene="yfiH"
                     /locus_tag="ACHOQQ_RS17615"
                     /old_locus_tag="pgaptmp_003523"
     CDS             complement(2794..3525)
                     /gene="yfiH"
                     /locus_tag="ACHOQQ_RS17615"
                     /old_locus_tag="pgaptmp_003523"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGU4471732.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3522..4502)
                     /gene="rluD"
                     /locus_tag="ACHOQQ_RS17620"
                     /old_locus_tag="pgaptmp_003524"
     CDS             complement(3522..4502)
                     /gene="rluD"
                     /locus_tag="ACHOQQ_RS17620"
                     /old_locus_tag="pgaptmp_003524"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGU4471733.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEAMRADFEEHKDEVDWL"
     gene            4637..5374
                     /gene="bamD"
                     /locus_tag="ACHOQQ_RS17625"
                     /old_locus_tag="pgaptmp_003525"
     CDS             4637..5374
                     /gene="bamD"
                     /locus_tag="ACHOQQ_RS17625"
                     /old_locus_tag="pgaptmp_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGU4471734.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5645..5986
                     /gene="raiA"
                     /locus_tag="ACHOQQ_RS17630"
                     /old_locus_tag="pgaptmp_003526"
     CDS             5645..5986
                     /gene="raiA"
                     /locus_tag="ACHOQQ_RS17630"
                     /old_locus_tag="pgaptmp_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGU4471735.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6089..6214
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6090..6137
                     /gene="pheL"
                     /locus_tag="ACHOQQ_RS17635"
                     /old_locus_tag="pgaptmp_003527"
     CDS             6090..6137
                     /gene="pheL"
                     /locus_tag="ACHOQQ_RS17635"
                     /old_locus_tag="pgaptmp_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HGU4471736.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6236..7396
                     /gene="pheA"
                     /locus_tag="ACHOQQ_RS17640"
                     /old_locus_tag="pgaptmp_003528"
     CDS             6236..7396
                     /gene="pheA"
                     /locus_tag="ACHOQQ_RS17640"
                     /old_locus_tag="pgaptmp_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGU4471737.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7439..8560)
                     /gene="tyrA"
                     /locus_tag="ACHOQQ_RS17645"
                     /old_locus_tag="pgaptmp_003529"
     CDS             complement(7439..8560)
                     /gene="tyrA"
                     /locus_tag="ACHOQQ_RS17645"
                     /old_locus_tag="pgaptmp_003529"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGU4471738.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8571..9641)
                     /gene="aroF"
                     /locus_tag="ACHOQQ_RS17650"
                     /old_locus_tag="pgaptmp_003530"
     CDS             complement(8571..9641)
                     /gene="aroF"
                     /locus_tag="ACHOQQ_RS17650"
                     /old_locus_tag="pgaptmp_003530"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGU4471739.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         2493 a         2626 c         2293 g         2398 t
ORIGIN      
        1 tcgctcactt ttcaggcaaa acatcttaaa tatagtcttt tccgtctaac ttatagacaa
       61 aaacgagccc cgaagggctc gttttatcat ttactggacg gcgacaatcc ggtcttcatt
      121 aacttccagg cgaatcactt tacccggaac caattcacca gacagtattt gctgtgccag
      181 cgggttttcg atctgctgct gaattgcacg tttcagagga cgtgcaccat agaccggatc
      241 gtaaccgttc tcgctcagca gtttcagcgc ctcgtcagaa atgtggattt cataaccacg
      301 ttcttccaga cgtttataca gacgtttcaa ctgaatctgc gcaatcgacg caatgtgctg
      361 ttcacccagc ggatggaaga ccaccacttc atcgatacgg ttaatgaatt ccggacggaa
      421 gttatggcta accacaccga gcaccagctc tttcatgtgc gcataatcca gttcaccgaa
      481 gcgttcctga atcaaatcgg aaccgaggtt agaggtcata atgacaaccg tattacggaa
      541 gtcgaccgtt ctcccctgcc cgtcagtcag acgcccatca tccagtacct gcaacagaat
      601 gttgaagaca tccggatgcg ctttttccac ttcatccagc aggataacgg aatacggacg
      661 acgacgcaca gcttcggtca ggtagccacc ttcttcataa ccgacatatc ccggaggcgc
      721 accaaccaga cgagacaccg agtgtttctc cataaactcg gacatatcga tacggaccat
      781 cgcctcgtcg ctatcaaaca taaagttcgc cagcgcctta caaagctctg ttttccccac
      841 accagttggg ccgaggaaca ggaatgaacc aatcgggcga tttggatccg ccagccccgc
      901 acggctacga cgaatagcgt tagataccgc atcaaccgct tcgttctgac caattacgcg
      961 atggtgcagt tcttgctcca tacgcagcag tttttcgcgc tcgctttcca tcatgcgaga
     1021 aaccggaatc cccgtccaac gcgccagcac ttcagcaatt tcggcgtcgg tcactttatt
     1081 acgcaataga cgcatagttt tgccttcgag ctgcgttgcg gcttccagtt gcttttccag
     1141 ttccgggatt ttgccgtatt gcagttcaga catccgcgcc aggtccccca cacggcgagc
     1201 ctgttcaata gcgattttcg cctgttccag ttccgcttta atggtctgcg taccagaaag
     1261 cgatgccttc tctgctttcc actcttcttc taactcggag tactgacgtt ctttgtcgct
     1321 cagttcttcg ttgagcatat ccaggcgttt tttactggct tcatcagact ctttcattaa
     1381 cgcctgttgt tccagtttga gctggatgat acgacgatcg agtcggtcga gttcttctgg
     1441 ttttgagtca atttgcatac gaatgctgga tgctgcttca tcgatcaggt cgatagcttt
     1501 atccggcagc tgacggtcag caatgtagcg atgagacaac gtcgccgctg caacaattgc
     1561 cgggtcagta atttgcacat ggtggtgcaa ttcgtaacgt tctttcaggc cacgcagaat
     1621 cgcaatggta tcttcaacag aaggctcggc aacaaacact ttctggaaac gacgttccag
     1681 cgcagcatct ttttcgatgt actggcgata ttcgtcaagc gtagtggcac ctacgcagtg
     1741 caattcacca cgcgccagcg ccggtttcag catgtttccg gcgtccattg cgccatcggc
     1801 tttacccgcg ccgaccatgg tatgtaattc gtcgataaac aggatgacgt tgccttcctg
     1861 tttggcaaga tcgttaagca cgccttttaa acgttcttca aactcaccgc gatatttcgc
     1921 cccagccacc agcgcgccca tatccagcgc cagtacccgg cggcctttca acccttccgg
     1981 cacttcgccg ttgataatac gctgcgccag accttcaacg atggcagttt taccgacgcc
     2041 gggttcacca atcagtaccg ggttattttt agtacgacgt tgcagcacct gaatggtacg
     2101 gcgaatttct tcatcacgac caatcaccgg atcgagtttg ccctgttcgg ctcgttcggt
     2161 aaggtcgatg gtatattttt tcaaagcctg acgttggtct tcagcacctt gatcgttcac
     2221 gctttcacct ccacgcattt gttcaatcgc ttgagtaatg ttggcggtgg tcgcccctgc
     2281 tgctttcagg atgtcggcca gcgtgccgcg agactcaagt gccgccagaa cgaacagttc
     2341 tgacgagata aagttatcac cacgtttttg cgccagcttg tcgcaaagat taagaacgcg
     2401 caccagatcc tgtgatggct ggacatcacc accagtacct tcaacctgcg gtaaacgatt
     2461 taatgcctga ttgatatctg tgcgcaactg gccagcattt atgccagctg atgttaacaa
     2521 aggactaacc gacccccctt cctgattcag cagggcgctc attaaatgaa gtggttcgat
     2581 gaattggttg tcgtgcccga gtgcaagtga ttgggcatcg gcaagagcaa gctggaattt
     2641 attagtaaga cgatccagac gcataactcc tcccataacg gatcaaagtt gctactggag
     2701 attaaatgag gtcatccctc aattattcaa ggttattgac cagattaatg tgaaaagaaa
     2761 atcacgcgta ccggatcgtc ttgattcttt aggttatatc agccaaatga aacttgccat
     2821 acgcccggtg gtcttgtcgc gacgataaga gaagaaagtc tcattttccg tatatgtaca
     2881 acggtcgccg ccgaaaattt gttcaacacc aacgctcgcc agacgctgcc gggcaagcag
     2941 ataaatatcc gccagatact tatcaccatg ctgaatgaaa gctgtactcg ctttggcgtc
     3001 tgctgccata aacgcctcgc gaacctccgc ccccacttcg aacgcgcgtg gaccaattgc
     3061 cggccctaac caggcgagaa tattttccgg attatcagca aaacaggaaa ctgtctcttc
     3121 cagcacgcct gcgcacagtc cacgccagcc agcatgagcg gcggcgactt ccgttcccgc
     3181 tcgattgcaa aacagcacag ggaggcagtc ggcagtcatc actgcgcaaa ccgtgccagg
     3241 cgtattgcta taagaggcat ctgctcgttt tgaggcataa ggttcgccag taagcttaag
     3301 cacatctttg ccgtgtacct gctcaagcca gaccggttta gaaggcaaat tgcccgcagc
     3361 aaaaagtcgc ttgcgattct cctcaacatg atccgggtta tcgccacaat gggcaccgag
     3421 gttgagcgag tcatacgggg gcaagctcac gccgccgata cgagtagagc tacaagccgc
     3481 aacacctttt ggcagcggcc actgcgggac aatcagctta ctcataacca gtccacttca
     3541 tccttatgtt cttcgaaatc ggcacgcatc gcctcaatca gctccaccat atcttgtgga
     3601 ataggcgcat gccattccat ttcgatgccg gagatcggat gataaagacg cagcatggtt
     3661 gcatgaagcg cctggcggtc aaacttacgc agcgtggaga taaacgcttc cgaagcacct
     3721 tttggcggac gcggacggcc accataaacc ggatcgccca ccagcggatg agtgatatgg
     3781 gccatatgca cgcggatctg gtgcgtacgt ccagtttcca gacgcaaccg cagacgcgtg
     3841 tgcacacgga agtgttccat gatgcgatag tgagtcaccg ctggtttgcc catcggatgc
     3901 accgccatgt gagtacgttt ggtcgggtgg cgactgattg gctcatccac cgtaccgcca
     3961 gcggtcatat gaccaatcgc caccgcttcg tactcacgag taatttcacg cctttgcaaa
     4021 gattcgacta aacgcgtctg agccggaacg gtttttgcca caaccatcag gccagtggtg
     4081 tctttatcca gacgatggac gatgcccgca cgcggtacat cggcaatagg cggataataa
     4141 tgaagcaacg cattcagtac cgtgccatcc gggttacccg cgccaggatg aaccaccagg
     4201 tcgcgcggtt tattaatgac gatgatgtct tcatcttcat agacgatatc cagcgggata
     4261 tcctgcggtt caaaacgcgc ttcttcttca atctctgcgt tgatggcaac ctgctcgcca
     4321 cccaatactt tttctttcgg cttatcacaa agtttgccgt taaccagcac gcgctggtcg
     4381 aggatccatt cttttattcg cgaacgtgaa taatccggga acatttcggc caaagcctga
     4441 tctaagcgtt gaccgagttg gttttcggac accgttgcag tgagctgtac tcgttgtgcc
     4501 atatatactg cttcttcgtt tatcgttggg gttttacggc tttgccgttt aatatagtgt
     4561 gctattgtag ctggtcttaa ccgggagcag gaacagagaa tctcccgtat tacattttga
     4621 ggaaagtcaa aacgtcatga cgcgcatgaa atatctggtg gcagccgcca cactaagcct
     4681 gtttttggcg ggttgctcgg ggtcaaagga agaagtacct gataatccgc caaatgaaat
     4741 ttacgcgact gcacaacaaa agctgcagga cggtaactgg agacaggcaa taacgcaact
     4801 ggaagcgtta gataatcgct atccgtttgg tccgtattcg cagcaggtgc agctggatct
     4861 catctacgcc tactataaaa acgccgattt gccgttagcg caggctgcca tcgatcgttt
     4921 tattcgcctt aacccgaccc atccgaatat cgattatgtc atgtacatgc gtggcctgac
     4981 caatatggcg ctggatgaca gtgcgctgca agggttcttt ggcgttgacc gtagcgatcg
     5041 agatcctcaa catgcacgag ctgcgtttag tgacttttcc aaactggtgc gcggctatcc
     5101 aaacagtcag tacaccaccg atgccaccaa acgtctggta ttcctgaaag atcgtctggc
     5161 gaaatatgaa tactccgtgg ccgagtacta tacagaacgt ggcgcatggg ttgccgtcgt
     5221 taaccgcgta gaaggcatgt tgcgcgacta cccggatacc caggctacgc gtgatgcgct
     5281 gccgctgatg gaaaatgcat accgtcagat gcagatgaat gcgcaagctg aaaaagtagc
     5341 gaaaatcatc gccgcaaaca gcagcaatac ataacagaaa cctgaaacac aaaacggcag
     5401 cccttgagct gccgtttttt tattctgtca gttgtgaaac tgaagcgatt tagtcactat
     5461 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
     5521 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
     5581 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
     5641 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
     5701 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
     5761 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
     5821 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
     5881 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
     5941 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
     6001 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
     6061 ctgtactaaa gtcacttaag gaaacaaaca tgaaacatac accgtttttc ttcgcattct
     6121 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
     6181 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
     6241 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
     6301 agcattactc gcagagcggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
     6361 gcatcgcccg gtacgtgata ttgatcgtga acgcgattta ctggaaagat taattacgct
     6421 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     6481 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     6541 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     6601 gtatgctgcg cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     6661 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     6721 ctccggtgcc ataaatgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     6781 cgagatgacg ttaactatcg accattgttt gttagtctcc ggcacgactg atttatccac
     6841 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     6901 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     6961 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     7021 tttgcaggta ctggagcgga ttgaagcgaa tcagcgacaa aacttcaccc gatttgtggt
     7081 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacga cgttgttaat
     7141 ggcgaccggg caacaagccg gtgcgctggt tgaagcgttg ctggtactgc gcaaccacaa
     7201 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     7261 ttatctggat attcaggcca atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     7321 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     7381 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     7441 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     7501 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     7561 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     7621 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     7681 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     7741 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagtg
     7801 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     7861 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     7921 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     7981 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     8041 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     8101 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     8161 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     8221 acaggcgtcc catctgaccg ccgccgccaa cgataactac cgggcgtaac gcaggacaaa
     8281 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     8341 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     8401 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcacctcgc
     8461 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     8521 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     8581 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     8641 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     8701 tcggaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     8761 ttgccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     8821 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     8881 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     8941 aggatcacat gtccgtccgg attcccctga gtttgtagca acgcaacctg acctgcctgg
     9001 ttaatgccaa caaaacggtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     9061 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     9121 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa caaatcgccc
     9181 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     9241 agctcaagca gcaatttacg cgcgatctgc agcccggctt ctacatcaaa agagccatcc
     9301 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     9361 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagggcttt aaatcgacga
     9421 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     9481 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     9541 tcttgttgca ggcttaatgg aaaagcggcc ttcagttgtg ccggagtcat taaaacctgt
     9601 tcgtcggtaa tatgtacgtt attcagcgcg tctttttgca tgatggcgat cctgtttatg
     9661 ctcgtttgcg atagttgatc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     9721 tttcaatcca tatttcgtac atttttcttt acagaagcaa tttattcgcg ctttcaaccc
     9781 ttgattctgt atagataaat ttacaccacc
//
LOCUS       DAZKFU020000179         9754 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZKFU020000179 DAZKFU020000000
VERSION     DAZKFU020000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.181"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(78..1205)
                     /gene="rlmC"
                     /locus_tag="ACHOQQ_RS17655"
                     /old_locus_tag="pgaptmp_003531"
     CDS             complement(78..1205)
                     /gene="rlmC"
                     /locus_tag="ACHOQQ_RS17655"
                     /old_locus_tag="pgaptmp_003531"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGU4471740.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            complement(1246..1734)
                     /locus_tag="ACHOQQ_RS17660"
                     /old_locus_tag="pgaptmp_003532"
     CDS             complement(1246..1734)
                     /locus_tag="ACHOQQ_RS17660"
                     /old_locus_tag="pgaptmp_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGU4471741.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(1794..2639)
                     /gene="potI"
                     /locus_tag="ACHOQQ_RS17665"
                     /old_locus_tag="pgaptmp_003533"
     CDS             complement(1794..2639)
                     /gene="potI"
                     /locus_tag="ACHOQQ_RS17665"
                     /old_locus_tag="pgaptmp_003533"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGU4471742.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPAERGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(2636..3589)
                     /gene="potH"
                     /locus_tag="ACHOQQ_RS17670"
                     /old_locus_tag="pgaptmp_003534"
     CDS             complement(2636..3589)
                     /gene="potH"
                     /locus_tag="ACHOQQ_RS17670"
                     /old_locus_tag="pgaptmp_003534"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGU4471743.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMNHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQFSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(3599..4732)
                     /gene="potG"
                     /locus_tag="ACHOQQ_RS17675"
                     /old_locus_tag="pgaptmp_003535"
     CDS             complement(3599..4732)
                     /gene="potG"
                     /locus_tag="ACHOQQ_RS17675"
                     /old_locus_tag="pgaptmp_003535"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGU4471744.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(4827..5939)
                     /gene="potF"
                     /locus_tag="ACHOQQ_RS17680"
                     /old_locus_tag="pgaptmp_003536"
     CDS             complement(4827..5939)
                     /gene="potF"
                     /locus_tag="ACHOQQ_RS17680"
                     /old_locus_tag="pgaptmp_003536"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGU4471745.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(6290..6766)
                     /locus_tag="ACHOQQ_RS17685"
                     /old_locus_tag="pgaptmp_003537"
     CDS             complement(6290..6766)
                     /locus_tag="ACHOQQ_RS17685"
                     /old_locus_tag="pgaptmp_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGU4471746.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(6854..7756)
                     /gene="rimK"
                     /locus_tag="ACHOQQ_RS17690"
                     /old_locus_tag="pgaptmp_003538"
     CDS             complement(6854..7756)
                     /gene="rimK"
                     /locus_tag="ACHOQQ_RS17690"
                     /old_locus_tag="pgaptmp_003538"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGU4471747.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(7817..8539)
                     /gene="nfsA"
                     /locus_tag="ACHOQQ_RS17695"
                     /old_locus_tag="pgaptmp_003539"
     CDS             complement(7817..8539)
                     /gene="nfsA"
                     /locus_tag="ACHOQQ_RS17695"
                     /old_locus_tag="pgaptmp_003539"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGU4471748.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(8523..8810)
                     /locus_tag="ACHOQQ_RS17700"
                     /old_locus_tag="pgaptmp_003540"
     CDS             complement(8523..8810)
                     /locus_tag="ACHOQQ_RS17700"
                     /old_locus_tag="pgaptmp_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGU4471749.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            8970..9227
                     /gene="grxA"
                     /locus_tag="ACHOQQ_RS17705"
                     /old_locus_tag="pgaptmp_003541"
     CDS             8970..9227
                     /gene="grxA"
                     /locus_tag="ACHOQQ_RS17705"
                     /old_locus_tag="pgaptmp_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGU4471750.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(9257..9634)
                     /locus_tag="ACHOQQ_RS17710"
                     /old_locus_tag="pgaptmp_003542"
     CDS             complement(9257..9634)
                     /locus_tag="ACHOQQ_RS17710"
                     /old_locus_tag="pgaptmp_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGU4471751.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
BASE COUNT         2523 a         2664 c         2375 g         2192 t
ORIGIN      
        1 gttattatta atcgatcgat cacgattatt attttggatg gatgtatgcc gaggtgatat
       61 tgatttgaat gatgtattta catttttacc agcagcgtca gcacttcata gtgcgcggtg
      121 tgcgggaaca tatcgaaaag ctgcacccgt tcaatgcgat aaccaggcag ttcgcggata
      181 tctttcgcca tggtttgggc gttacagctg gagtagatga taaaacgcgg tgccatcgtt
      241 gagagataat cacacagcgg tttaccaatg ccgcggcgcg gcgggttaac cagcaccagc
      301 tccggcacct ccccctgagc ggtggcaaac tgagtggagt ccagcgcctg aaattgcaaa
      361 cgcgttaagc ccagttcagc agctgactgc tttgcacagg caatggcctc tggcgcaatt
      421 tcgatcccgg ttaactgcat gtcaggcgtc gcgcagtgta aaccaaagcc ccccacgccg
      481 cagaacagat cccacatatg tttaaccggc agctgtcgta cccagtcgcg cgcggtagcg
      541 tacagctggc tggcgaccgc cggattagtc tggaagaaac tttgcggacg gatccacagc
      601 ggcacatcgt taaaacgctc tgccagcgcc tgttgttcgg tcaggtagat ctccgtctcc
      661 ccttccataa tcgccatatg taccggctga atattgacgg tgataacttt tagctgcggc
      721 aattgtgcct gtaaccacgg caatgcctta cgcagttgtg ccagtttggt ttcagaacgc
      781 agtacaaagc gcagcatcat gccgccatcg ctctggcttt cagtcagcag aatgtatttc
      841 agttcgccac gcttacgcgc cacgttgtag ggcgttaacc ccgctcgggc gataaacggt
      901 tttagcgccg caaaaacggg cgcaaatgag gcaggataaa gcgggcagtc acaaaggtct
      961 tccggtgtgc cgtcgcgatg cagcataccg agcagtggtt tttcaacgct accactcacc
     1021 accattttgg ctttattacg aaacccttgt tccgggcctg acaccggcgc gcaccattcc
     1081 tcaaccggaa agtcggcgag cagattttta agatcggcgg ttttagcgga gagttgctct
     1141 ggaatcggct gcgttatcca ctgacaggaa cgacagcgac ccgcgtcgta aagtgcgcac
     1201 tgcatacatt gaccttcaca tcatcagggg cgacgattat acacattatt gcaactggaa
     1261 gaaccgccga ctggtcgagg gaacgaacag cagcatcagg atgagcatat ccggcagctt
     1321 ctgcagcatc aggctatgga agatttcacg ttttgattca ccgggaatgc tgaacagctc
     1381 cggataaccg taccccagcg aagccgccca caagtaactt gcggccgtga tttgtgtcag
     1441 cagatatagc catcgcgccc agcgacgccc tttcaccagt gaaaacgcac accagatctc
     1501 aatgaaaacc agcaccagac tgcttaaaaa gaccagcgtt aaactccagg tttgtacgct
     1561 gcgatgaatg aactcgccaa taccgcgcac gcccagcgta ttgaaaatca tcagcacgtc
     1621 gaggccacgg atcataataa tggcgagcgc cgccacctgc accagcgcag gcacattcag
     1681 gcgagcatga gatgaagatg ttttcttaaa aaatcccaac gtttcgtctt ccatgaaaac
     1741 aatgccgcga catacgcggc attatgtagc caggttggca aattttagtg tcttcagcca
     1801 cgtcttgcac gctggatgtc gcgtatccgc tgtttttctg cgcgagccat cagataccag
     1861 gcgataaatc cgacaattcc gaccgcgccg aggatcaatg ttgccagggc gttgatttcc
     1921 ggattcaccc ccatccgcac gctggaaaag accagcatcg gtaacgtggt ggctcccggc
     1981 ccggaaacaa agctggcgat caccagatca tcaagcgaca aagtaaaagc cagtaaccag
     2041 ccagaaatga tcgcgggcat gatcatcggt agcgtaatga cgaaaaacac tttcagcggc
     2101 gtcgcaccga gatccatcgc tgcttcttct atcgagcgat ccagttcccg cagacgcgac
     2161 gaaataacga ctgccacata agccgtacag aaagtgacat gcgccagcca gatggtaagc
     2221 ataccgcgtt ccgcaggcca gccaatggca tgagcaagcg cgacgaataa taacaacagc
     2281 gacaagcccg tgatgacatc tggcattacc agcggcgcgg tgatcataaa ggcaaagcca
     2341 tttgatccgc gaaacctgcc aaagcgcacc agcaccaccg ccgcaatagt tccgaggatc
     2401 gccgccgccg tcgccgcaca ggccgcaatt gtcagactta aaccaaccgc actcatcatc
     2461 gcgtcatcgc gcaataattc gccataccag cgcgttgacc agccggccca caccgtcacc
     2521 agcttcgagc tgttaaacga atagataacc agcatcagca ttggcgcgta gagaaaggtg
     2581 aagcccagca gcaaaatcac aatccgccag ggcgaacgaa ctaccggtaa attattcatc
     2641 cgtgttctcc cacgcttttt tgctggtgtt tgtggaacca cattatcggc acaattagca
     2701 gcagcaacat gatgatcgct accgccgagg ccaccggcca gtcgcggtta ttgaaaaact
     2761 cttgccatag cacgcgcccg atcatgatgc tgtccgggcc accgagcagt tccgggatca
     2821 caaactcgcc cacagccggg ataaacacca gcatcgatcc ggcaataatc ccacctttag
     2881 tcagcggcac gatcacagta aagaacgttt tcagcggtcg tgcaccgaga tccagcgctg
     2941 cttccaccag cgaataatca atacgaatca acgcggtata aatcggcagc accataaacg
     3001 gcacgtaggc gtaaacaatg ccaatataaa ctgccagatt ggtatgaaga atggtcagcg
     3061 gttgatcgat aacccccagc cacagcagaa aattattcag cacaccgttg ttttttaata
     3121 ttcccatcca ggcgtagaca cgaatcagaa acgaggtcca cgacggcagg atcaccagta
     3181 ataataagat attacgggtc gaaggcttac tgtgcgccac cgcccacgcc agcggatagc
     3241 cgatcagtaa acagcaaata gtcgaaatcg ccgccacctg taacgactgg agataagcat
     3301 cgaaatagag cggatcgtcg gtcagttgca gaaaattacc gagattaaga gtgatggaaa
     3361 attgcccgtc agcccactcc atcagttcgg tataaggtgg aatagcgcgc gccatctctg
     3421 ccaggcttat tttaaagaca atcagaaatg gcagcagaaa cagcaagatc aaccagatat
     3481 acggcaacgc aatgaccagt ttgcgcccat ggttcatttg caactgcgac aaccacagct
     3541 taaatccgcc cggcggtttc gactgggcag caggttcaag tgtactcatc tttgctcctt
     3601 aaaccgtaag caccacacag ctgtccactt cccagcacaa acgcacttcg tcgccccagg
     3661 tcggtaaccc tttacgatga cgatgggcgt tttgtagctg ggcgctaatc atctgcccac
     3721 ttttcagacg aacgtgatac accgaaagat cgccgagata ggcaatgtgt atcacctccc
     3781 ccaccgcgaa gttacaacca ttggcgggcg gctcttcgca aagcatgatt ttttccggac
     3841 gcagcgctac gtgtaccggc acgttatcga ccactgaagc atccgcatcg actttcagtg
     3901 gatgcaccag ccccggcgaa taaagcacca gaccatcttc ctgacgctct ttgagtacac
     3961 cttcaaagac attaaccgag ccaataaatt cggcgctata gcgggtagtc ggatgctcgt
     4021 agatctcttc cggttcgcca atctggacaa atttcccacg attcataatg gcgatacgcc
     4081 ccgccatggt catcgcctct tcctgatcgt gagtaaccat cacgcaggtc acaccgacgc
     4141 gctccagaat atccaccact tcaagctgca tccggtcacg cagcttttta tccagcgcgc
     4201 ccatcggctc atcgagcagt aatagtttcg ggcgtttggc aaggcttcgg gccagggcca
     4261 cacgctgtcg ctgaccaccg gaaagctgat gcggtttgcg tttggcgaac tcctgcatat
     4321 gcacaaggcc gagcatctca ttgacccggc tggcaatttc cgctttcggt agtttgtcct
     4381 gtttcaggcc aaaagcgatg ttctgttcca cggtcatatg cggaaacagc gcgtaagact
     4441 gaaacatcat attgatgggg cgcaggtaag gcggcacctg tgacaaatcg acgccatcga
     4501 gcataatctg cccggctgaa ggttgttcga aacctgccag catacgcagc agcgtggact
     4561 tgccacagcc ggatgcgccc agcagcgcga agatttcacc tttgtagatg gtcaggctga
     4621 catcatccac cgcatgttga ccatcgtagg atttggtcag gttgcggatt tctaatagcg
     4681 gcgtcagcgc cttacgggtt ttcgcctgcg ggcgagggat agcgtcattc acggctgctc
     4741 tccggcattg aatgtgtgca agcgcaccat aatggtgcga attgccggcg ggtgtggtgc
     4801 gcccgtccgg catctaaggc tgcggattat tttccgctct tcactttggt ccacgcgcgg
     4861 gtgcgcacac ggtcgatttt cggatcctgt actttcagag tgaacaactt cgcgcggaca
     4921 tccgcaggcg gataaatacc cgggttatca cggacttccg cactcaccag cggcgtggct
     4981 gctttattgg cgttagcata gaacacatgg tcagagatat gtgccaccac atcaggacgc
     5041 agcaggtaat tcaggaactg ataggcttcg tctttatttt tggcatccgc aggcatggcg
     5101 aatacatcaa agaacgccat cgccccttct tttggaatcg agaacgagac attcacgcca
     5161 ttcttcgctt ccttcgcgcg gtttgacgcc tgccagacat cacctgccca gccgatagcg
     5221 acgcaaatat cgccgtttgc caggtcgtta atgtattgag atgaatggaa ataacgaatg
     5281 ttcgggcgca gctttaacag cagatcggta gccggtccgg tgtaatcatc cgctttagtg
     5341 ctgttgggat ctttgccgag ataattcagc acggtagcaa aaacttcttc tggcgcatcg
     5401 aggaaagata caccgcagct tttcagtttt tccagatttt caggcttgag gatcaagtcc
     5461 cagctatcga cgggcgcgtt ttcgcccagc accgctttga ctttatcgac gttatagcca
     5521 atcccggtcg tcgcccacat atagggcata gcaaatttat tgtcgggatc gtgtttggcg
     5581 accaacttca gcagttccgg gtcgagattc ttccactccg gcaatttgct tttatccagc
     5641 ggctggaaaa ctcccgctgt cagctggcgt tcaagaaagc ttgctgacgg caccaccagg
     5701 tcaaagccgg tgctcccggc cattaatttg ccttccagta cttcgttaga gtcgaaaaca
     5761 tcgtagacga ctttaatacc ggtttctttt tcaaagttag ccaccgtgtc gggggcgata
     5821 taatcagacc agttataaat gtggagtgtt ttttgttcag ccgcgagcgt gccgacagag
     5881 acggccatca gagctcccgc aaccagaccc gaaagccatt ttttatttaa ggcggtcata
     5941 tctctttcct tctgaaagtt cgttaacaaa cgaacaaaag ttgtcacact gaaagatatg
     6001 caataaacgt gcatattttt tatcggcgcg gtgtaagaaa agagaactct ctcccggcca
     6061 gcactgctcg ctatataaaa gcatcgcaag aataacttta gccagggttt gagactatag
     6121 ctcggattaa aaaacggtat ttaacaaatt tttatgcgta tttgctctat gtgataaagc
     6181 caaatcgaca caagagaagt gaaataatct ttaagaaaag gttaaaaaca gcagataatt
     6241 ttgcagcgcg cagccactgc accagcgact gcgtgctcga aaggagttat cagtggagga
     6301 aatagttttc ggtaacgttg ttttgccctt catcgtcagt cggtaacagc agttgatggg
     6361 cattagcttc aagaatgacc atcgaaatct gctcttcgct ctgacgcacg aaccatgcaa
     6421 actgctcata ggttacgccc tgcataacgg ataaagactg gcaaaccacc agctttggca
     6481 gattatcatc ctgcatatca agaaatgctt tcacggtcag cgaactggca ttgatggcag
     6541 ataaatccgc cgccagcggc aatacggctg aaggtcggac ttccgccatg gcagaaaaca
     6601 ggatcgtgtt atcgatcaga tcgattttgg catcaaagac accgtcgaaa ttctgcatat
     6661 ggggcagatg cagagcctga cagttatcac attcaaaaaa actcatcccc aggtcatcga
     6721 gccattgacg cagcgtatcc agaccaggaa cgaccagcga tgtcataatt ttgtacaacc
     6781 tctttcgata aaaagaccgg cacagcttac gcaaaaagcg caggcaaaac catgatcagt
     6841 aatgtgattg cgactaacca cccgttttca ggcaatattc tgtcgtagcg tggcgttcga
     6901 tccagcggat cattttaccc gcgatgtcga taccggtggt tttttctatt ccttccagcc
     6961 ccggcgacgc attcacttcc atcaccaacg gcccgcgatt agcacgcaga atatccacac
     7021 cagcaacgtc cagcgccatc gttcgcgcgg cttttatcgc gatttcacgc tcctgtggtg
     7081 tgatactggc gacgcttgcc gcgccgccac gatgcaaatt ggaacgaaaa tcgccctctt
     7141 tcgcccgccg ttcaatcgca gcgaccactt catcgccaac aaccagacag cggatatcgc
     7201 acccttgcgc ctctttgata tattcctgca ccagaatatg cgcgttcaga ccgcggaaag
     7261 cgtcaatcac gctttccgcc gcctgacgtg tctccgccag cacgacgcca attccctgcg
     7321 tgccttcaac caacttgacc accagaggcg caccaccgac catgtcgatt aaatcgctgg
     7381 tatcatccgg cgaatgcgca atgcccgtga caggcaggtc gatgccctga cgcgccagca
     7441 gttgcatgga acgcaatttg tcacgcgccc gggcaatggc gaccgactca ttgagcggat
     7501 agctccccag catctcgaac tggcgcagtg ccgccgtccc ataaaaggta atggcggtgc
     7561 caatacgcgg gatcactgcg tcaaaatggg gtaacttgcg gcctttgtag tgaatagaag
     7621 acgccgcagg atttatgttc atgtagcaag aaagcggatc aagaatttca accaggtgac
     7681 cgcgctgtat agcggcttca cgcagccgct tacacgaata gagcgttcca tcccgggaca
     7741 atatggcaat tttcaccctg cacctctctg tcagacctgg tcaaaagccc gcgtatcata
     7801 catcgacgtg gcggttttag cgcgtcgccc aaccctgttt gtgcaaataa tccagaataa
     7861 atgggcggct ttctttaatg attgttcggc ggatatgatc gctccaggta tcccggcgat
     7921 tattgctgcc acgggtgagg taatattccg ccagttgctc gtcatactgc gccagtgcgt
     7981 ctttatccag cggttgatag ctgttttcat gcaccaaaat ggaggccggt aaacgcggct
     8041 tgagatccgg attatccgca ggccagccaa ggcacagccc aaacagcggc agaacatgct
     8101 gcggtaattt cagcagtttc gtcaccgctt caatattatt gcgcaggccg ccgatatata
     8161 cgccgcccaa tcccagcgat tccgctgcgg ttaatgcatt ctgcgccatc attgccgtat
     8221 caacgacacc gagcaacagc tgttccgcca ggccgagctg agcatccgga cagatctgta
     8281 aatggcggtt aaagtcggca cagaacaccc agaactccgc cgcttgcgct acgtgttttt
     8341 gcccgccggt cagcgtcacc agctcttcac gtaatgcttt gtcggtaatg cgaataatgc
     8401 tactacactg caaaaaactg gaactggacg tcgcacgggc gctgttaata atcgcctcac
     8461 gctgcgcttc ggaaatgggt tcatcagtga aatggcgaat ggagcgatgg ccacaaataa
     8521 gttcaatggt tggcgtcatt atctttttct ctttctgaac gtgaatattg cggtggacgg
     8581 ttcatcagct gtggggcaag acgttttgcc acctgaagaa taaccaccac cgcagcggga
     8641 agcatgagca aaacaccgag aaaaatcatc agaatctgca cttctggccg agaaaatggc
     8701 tcaggcagcg acagggagtc gcttaccgac agcagcgcca ccgccagcaa catcattccg
     8761 ataaattcca gtatcaacac gcttttaggc aatttaccga tcgcgcgcat acgcttccct
     8821 ctgcaaagtg agccttcagt ctaaaacttt tcactgtatt gtgtttaaca gttatagctt
     8881 ttagcaatta atgcaacagg ttaaacctac tttcagcgaa tacattttag cgtgatcatt
     8941 acaggcataa atctatgagg agagaaataa tgcaaaccgt tatttttggt cgcccgggtt
     9001 gcccttactg tgtgcgtgca aaagatctgg ctgagaaatt gagcaatgaa cgcgatgatt
     9061 ttcagtatca gtatgtagat attcgtgcgg aagggatcac taaagaagat ctacaacaaa
     9121 aggcaggtaa acccgtagaa accgtgccgc agatttttgt cgatcagcaa catatcggcg
     9181 gctataccga ttttgctgca tgggtgaaag agaatctgga cgcctgatcg tctgacaagc
     9241 cctcgcgttg agggctttac tgattttttc tgtgctgtgg tttaaacaag ctactgataa
     9301 ataagaaaca cagtgccccc agcgcacacc agaacaccgc gcttagtaac catgccagct
     9361 cttgccagaa tgagcgggtc ggtgaaaaaa acagccgcat aataagcatt gaacagggtg
     9421 ccgccagcat tgcgccaaat agaggtttca ggacttctct acgctgtgaa aagaaactgg
     9481 caactgctcc aggaagaatg aaaaacagca agccgatttc aggatgcccg gcagcccgaa
     9541 aagcgccttt catgtgcgtc gccagaaaaa ggcacaccac aatgaagagg acaaaacagc
     9601 agattgcccc cgcccaacgt tgtttatgtt tcactcgttc ctcctgacac tgcgtctatc
     9661 gaacacattt ttcgccagtg tggcgttcag taagataaag ccgcttcgca ttccatgcta
     9721 atataggcca acgcaattca tatagccgtt gata
//
LOCUS       DAZKFU020000180         9687 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZKFU020000180 DAZKFU020000000
VERSION     DAZKFU020000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.182"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..860)
                     /gene="rarD"
                     /locus_tag="ACHOQQ_RS17715"
                     /old_locus_tag="pgaptmp_003543"
     CDS             complement(<1..860)
                     /gene="rarD"
                     /locus_tag="ACHOQQ_RS17715"
                     /old_locus_tag="pgaptmp_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGU4471752.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDA"
     gene            complement(912..1379)
                     /gene="yigI"
                     /locus_tag="ACHOQQ_RS17720"
                     /old_locus_tag="pgaptmp_003544"
     CDS             complement(912..1379)
                     /gene="yigI"
                     /locus_tag="ACHOQQ_RS17720"
                     /old_locus_tag="pgaptmp_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGU4471753.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1544..2413
                     /gene="pldA"
                     /locus_tag="ACHOQQ_RS17725"
                     /old_locus_tag="pgaptmp_003545"
     CDS             1544..2413
                     /gene="pldA"
                     /locus_tag="ACHOQQ_RS17725"
                     /old_locus_tag="pgaptmp_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGU4471754.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2546..4375
                     /gene="recQ"
                     /locus_tag="ACHOQQ_RS17730"
                     /old_locus_tag="pgaptmp_003546"
     CDS             2546..4375
                     /gene="recQ"
                     /locus_tag="ACHOQQ_RS17730"
                     /old_locus_tag="pgaptmp_003546"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGU4471755.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4439..5059
                     /gene="rhtC"
                     /locus_tag="ACHOQQ_RS17735"
                     /old_locus_tag="pgaptmp_003547"
     CDS             4439..5059
                     /gene="rhtC"
                     /locus_tag="ACHOQQ_RS17735"
                     /old_locus_tag="pgaptmp_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGU4471756.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5121..5741)
                     /gene="rhtB"
                     /locus_tag="ACHOQQ_RS17740"
                     /old_locus_tag="pgaptmp_003548"
     CDS             complement(5121..5741)
                     /gene="rhtB"
                     /locus_tag="ACHOQQ_RS17740"
                     /old_locus_tag="pgaptmp_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGU4471757.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5852..6874
                     /gene="pldB"
                     /locus_tag="ACHOQQ_RS17745"
                     /old_locus_tag="pgaptmp_003549"
     CDS             5852..6874
                     /gene="pldB"
                     /locus_tag="ACHOQQ_RS17745"
                     /old_locus_tag="pgaptmp_003549"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGU4471758.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6882..7682
                     /gene="yigL"
                     /locus_tag="ACHOQQ_RS17750"
                     /old_locus_tag="pgaptmp_003550"
     CDS             6882..7682
                     /gene="yigL"
                     /locus_tag="ACHOQQ_RS17750"
                     /old_locus_tag="pgaptmp_003550"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGU4471759.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7758..8657
                     /gene="bioP"
                     /locus_tag="ACHOQQ_RS17755"
                     /old_locus_tag="pgaptmp_003551"
     CDS             7758..8657
                     /gene="bioP"
                     /locus_tag="ACHOQQ_RS17755"
                     /old_locus_tag="pgaptmp_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGU4471760.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8545..9498)
                     /gene="metR"
                     /locus_tag="ACHOQQ_RS17760"
                     /old_locus_tag="pgaptmp_003552"
     CDS             complement(8545..9498)
                     /gene="metR"
                     /locus_tag="ACHOQQ_RS17760"
                     /old_locus_tag="pgaptmp_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGU4471761.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
BASE COUNT         2245 a         2480 c         2606 g         2356 t
ORIGIN      
        1 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
       61 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
      121 tactggaaaa agcctaacgt tgagagacgc aggcgcgtgg cagcggcagt aaaacacaac
      181 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
      241 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaatcgc cgccacgggc
      301 agcagccaca tggtttcaat taacatgccg gtttgtgctt ctaccgcaat cttcttgcgc
      361 accaggccgt agaaggcaaa actaaatgcc agccccagcg cgataatagg tagcgaacca
      421 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
      481 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
      541 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
      601 cagttgccac caatcagtac ggcagagact gccagcataa aaattttctg tggcgtctga
      661 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
      721 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
      781 aagtacgctg gggctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
      841 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
      901 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
      961 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     1021 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     1081 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     1141 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     1201 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     1261 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     1321 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     1381 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     1441 ctactttagg aagggataac taacggcaat ggagttaagt atgtaaaata gcccgcagaa
     1501 aaacattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     1561 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     1621 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     1681 tgacaatccg ttcacgctct atccttatga caccaactac ctcatctaca cccaaaccag
     1741 cgatctgaac aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     1801 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     1861 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     1921 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     1981 ccgttttgca ggttggacgc tgcgcgatgt agagatgggg tataaccacg actctaacgg
     2041 gcgttcagac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     2101 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     2161 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     2221 ggtgctcagt gcgaaaggac agtacaactg gaacaccggc tacggcggcg cggagttagg
     2281 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     2341 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtggggg ttatgctaaa
     2401 cgatttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaatccagca tccaggatta
     2461 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     2521 tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     2581 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgcccgg gccaggaaga
     2641 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     2701 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     2761 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     2821 ggcgtgcctt aactcgacgc aaacccgcga acagcaactt gaagtgatga caggctgccg
     2881 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     2941 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     3001 ccaatggggc cacgatttcc gcccggaata tgccgcgctc ggtcagttgc gccagcggtt
     3061 cccgacgctg ccgtttatgg cgctgaccgc cacagccgac gacaccacgc gccaggatat
     3121 cgtgcgcctg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     3181 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     3241 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     3301 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     3361 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     3421 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     3481 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     3541 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     3601 ggaagagaag ccgcaggggc agttgcagga tatcgagcgc cacaaactca atgcgatggg
     3661 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     3721 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     3781 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     3841 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     3901 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     3961 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     4021 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     4081 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     4141 tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     4201 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     4261 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     4321 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     4381 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     4441 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gtcccggtcc
     4501 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     4561 cgtgctgggt attacctgcg gcgttatggt ttgggcaggg attgcgttgc ttggcctgca
     4621 tttgattatc gaaaaaatgg cctggctgca tacgctgatt atggttggcg gtggcctgta
     4681 tctctgctgg atgggttacc agatgctgcg cggcgcgctg aaaaaagagg cggtttctgc
     4741 acctgcgcca caggtcgagc tggcgaaaag tgggcgcagt ttcctgaaag gtttactgac
     4801 caatctcgct aatccgaaag cgattatcta ctttggctcg gtgttctcat tgtttgtcgg
     4861 tgataacgtt ggcactaccg cgcgctgggg catttttgcg ctgatcattg tcgaaacact
     4921 ggcgtggttt accgtcgttg ccagcctgtt tgccctgccg caaatgcgcc gtggttatca
     4981 acgtctggcg aagtggattg atggttttgc cggggcgtta tttgccggat ttggcattca
     5041 tttgattatt tcgcggtgat gccagacgcg tcttcagagt aagtcggata aggcgtttac
     5101 gccgcatccg acattatttt tcacgcatgc ctcgccgatg ctaacagcgc tcccaccagc
     5161 ataaacaacg agccgaaaat cttattcagc gccttcatct gctttggtcc tttaatccat
     5221 agagcaatcc gttgagcaag ggtggcgtaa ccgatcatca caataatatc gaccacaata
     5281 gtggtgacgc cgagcacgat atactgcatc agttgcggct gttgcggcat gatgaattgc
     5341 ggaaatagcg cggccagaaa cacaatactt ttgggattgg tgagattcac aaaaactgcg
     5401 cgctggaaca aatgtcgacg cgattgagta gaggccagcg atttaagatc aattgcgcca
     5461 gcggcgcgcc attgctgtat tcccagccaa atcaagtaag ccgcgcctgc ccacttcaac
     5521 acttcaaacg caatcactga gcgggaaaat agcgtcccca accccacgcc aaccagcaca
     5581 atatgaatcg ccagtccggt ctgaagccca gcaatagacg ccaccgcgcc gcgataaccg
     5641 tggttgagcg aggtggtcat agtgttgatt gcaccagagc ctggcgacag gcttaaaatg
     5701 atcgatgtca gcaggtaggc aaaccaccat tctaaggtca tgatgaactc ccggtgtgtc
     5761 tatttttgtg ccacaatacg ctactgtcgc agcgttgtgt caggcacgct aaaaaaaacg
     5821 attttacgtg gtttaagagg cagattaccc gatgtttcag cagcaaaaag actgggaaac
     5881 aagagaaaac gcgtttgctg cttttaccat gggaccgctg actgatttct ggcgtcagcg
     5941 tgatgaagca gagtttactg gtgtggatga cattccggtg cgctttgtcc gttttcgcgc
     6001 acagcaccat gaccgggtgg tagtcatctg cccggggcgt attgagagct acgtaaaata
     6061 tgcggaactg gcctacgatt tgttccattt ggggtttgat gtcttaatca tcgaccatcg
     6121 cgggcaggga cgttccggtc gcctgttagc cgatccgcat ctcgggcatg ttaatcgctt
     6181 taatgattat gttgatgatc tggcggcatt ctggcaacag gaggtccagc ccggtccgtg
     6241 gcgtaaacgc tatatactgg cacattcgat gggcggtgcg atctccacat tatttctgca
     6301 acgccatcca ggtgtatgtg acgccattgc gctaactgcg ccaatgtttg ggatcgtgat
     6361 tcgtatgccg tcatttatgg cacggcagat cctcaactgg gccgaagcgc atccacgttt
     6421 ccgtgatggc tatgcaatag gcaccgggcg ctggcgcgcg ttgccgtttg ctatcaacgt
     6481 actgacccac agcagacagc gatatcgacg taacttacgc ttctatgctg atgacccaac
     6541 gattcgcgtc ggtgggccga cctaccattg ggtacgcgaa agtattctgg ctggcgaaca
     6601 ggtgttagcc ggtgcgggtg atgacgccac gccaacgctt ctcttgcagg ctgaagagga
     6661 acgcgtggtg gataaccgca tgcatgaccg tttttgtgaa ctccgcaccg ccgcgggcca
     6721 tcctgtcgaa gggggacgac cgctggtaat taaaggtgcg taccatgaga tcctttttga
     6781 aaaggacgca atgcgctcag ttgcgctcca cgccatcgtt gattttttca acaggcataa
     6841 ctcacccagc ggaaaccgct ctacagaggt ttaaatttct tatgtaccag gttgttgcgt
     6901 ctgatttaga tggcacgtta ctttctcccg accatacgtt atccccttac gccaaagaaa
     6961 ccctgaagct gctcaccgcg cgcggcatca actttgtgtt tgcgaccggt cgtcaccacg
     7021 ttgatgtggg gcaaattcgc gataatctgg agattaagtc ttacatgatt acctccaatg
     7081 gtgcgcgcgt tcacgatctg gatggtaatc tgatttttgc tcataacctg gatcgcgaca
     7141 ttgccagcga tctgtttggc gtagtcaacg acaatccgga catcattact aacgtttatc
     7201 gcgacgacga atggtttatg aatcgccatc gcccggaaga gatgcgcttt tttaaagaag
     7261 cggtgttcaa atatgcgctg tatgagcctg gattactgga gccggaaggt gtcagcaaag
     7321 tgttcttcac ctgcgattcc catgaacaac tgctgccgct ggagcaggcg attaacgctc
     7381 gttggggcga tcgcgtcaac gtcagtttct ctaccttaac ctgtctggaa gtgatggcgg
     7441 gcggcgtttc aaaaggccat gcgctggaag cggtggcgaa gaaactgggc tacagcctga
     7501 aggattgtat tgcgtttggt gacgggatga acgatgccga aatgctgtcg atggcgggga
     7561 aaggctgcat tatgggcagt gcgcaccagc gtctgaaaga ccttcatccc gagctggaag
     7621 tgattggtac taatgccgac gacgcggtgc cgcattatct gcgtaaactc tatttatcgt
     7681 aatcgttctt tatttggtca gttgtcaacc tgatacttcg ctacaatgga tactcgttaa
     7741 tcaaagagtt ttccattgtg gcgctactta tcatcaccac gattttgtgg gccttctcct
     7801 ttagttttta tggcgagtac cttgcggggc acgtcgatag ctattttgcg gtgctggtgc
     7861 gcgttggcct ggcggcactc gtttttctgc cgtttctgcg tacccgtggc aatagcctga
     7921 aaacagtcgg cctgtatatg ctggtgggcg cgatgcagct cggcgtgatg tatatgctga
     7981 gtttccgcgc ttatctctac ctgacggttt ccgaactgct gctgttcacc gtactgacgc
     8041 cgctctacat cacgctgatt tatgacatca tgagtaagcg ccgtctgcgc tggggatatg
     8101 cttttagtgc cttgttggcg gtgattggtg ccgggattat tcgctatgat caggtcaccg
     8161 accatttctg gactggcttg ttgctggtgc aactctccaa tatcactttt gccatcggca
     8221 tggtgggtta caaacgcctg atggaaactc gcccgatgcc acagcataac gcctttgcgt
     8281 ggttctatct tggcgcgttc ctggtggcgg taattgcatg gttcttgctg ggaaatgcgc
     8341 agaaaatgcc gcaaaccacg ttgcaatggg gcattctggt gtttcttggc gtggtggctt
     8401 ccgggattgg ctactttatg tggaactacg gcgcgacgca ggtggacgcc ggaacgctgg
     8461 gaattatgaa taatatgcac gttccggcag ggctgctggt aaacctggct atctggcacc
     8521 aacagccgca ctggccaacg tttattacag gcgcgctggt gatcctggcc tcactgtggg
     8581 tgcatcgtaa gtgggtcgct ccgcgctctt cacaaacggc agatgatcgc aggcgtgatt
     8641 gcgcgctgag cgaataaacg cttccgtaac tggctgacgc tgctcgccat cgcgcacggc
     8701 ggcgtacagt cggctccaca agccttcgcc cagggttttg gtcaccacca gaccctggcg
     8761 ctcaaaactc tctactaccc aatgcggcag ggcggcaata cccatccgcg cggcaaccat
     8821 ctgaatcaac aataaggtgt tatcgacgct tttcagtgac gggcttacgc ctgccggctg
     8881 aagaaaatgc cgccagacat ccagtcggct acgctgcacc ggataaatca acagcgtctc
     8941 gctggcgaga tcttccggtg taatacgcgt ttttgccgcc aatggatggt caggtgctaa
     9001 caccaggcgt acttcataat cgaacatcgg cgaataatgc aggccactgc gcggcaggat
     9061 atcggaggtc atcaccagat ccagctcgcc ctgttgcaac gcgggctgcg ggtcaaacgt
     9121 cacgcccgat ttaaaatcca tctctacctg cggccagttc ttatggaaat tttctaacgc
     9181 aggcgtcagc cactgaatac agctatggca ctcaatggca atgcgcagac gcgtctgctg
     9241 cggttcattg caggcttgca gtgcctggct gatttgcggc agcacctggt ttgccagttg
     9301 caacaggatt tctccctgcg gtgtaaagcg tagcggctgg ctcttacgca caaagagccg
     9361 gaagccaagg cgttgttcca gatcgctaaa ctggtgagac agggcggatt gcgtctgatg
     9421 caacgtcgcc gcagcggctg cgagagagcc gcagttccgc aacgcttgta gcgttttcag
     9481 gtgttttact tcgatcatga aagtccttca cttcggcatg aataatttgc gcttgaggaa
     9541 tatacagtaa ccgccaatta tggatgtgta aacatctgga cggctaaaat ccttcgtctt
     9601 ttaaatttat ggtgcgttgg ctgcgtttct ccaccccggt cacttacttc agtaagctcc
     9661 cggggatgaa taaacttgcc gccttcc
//
LOCUS       DAZKFU020000181         9540 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZKFU020000181 DAZKFU020000000
VERSION     DAZKFU020000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.183"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..107
                     /locus_tag="ACHOQQ_RS17765"
                     /old_locus_tag="pgaptmp_003553"
     CDS             <1..107
                     /locus_tag="ACHOQQ_RS17765"
                     /old_locus_tag="pgaptmp_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4471762.1"
                     /translation="ISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(91..729)
                     /gene="ytfB"
                     /locus_tag="ACHOQQ_RS17770"
                     /old_locus_tag="pgaptmp_003554"
     CDS             complement(91..729)
                     /gene="ytfB"
                     /locus_tag="ACHOQQ_RS17770"
                     /old_locus_tag="pgaptmp_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGU4471763.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            948..1568
                     /gene="fklB"
                     /locus_tag="ACHOQQ_RS17775"
                     /old_locus_tag="pgaptmp_003555"
     CDS             948..1568
                     /gene="fklB"
                     /locus_tag="ACHOQQ_RS17775"
                     /old_locus_tag="pgaptmp_003555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4471764.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1877..3289
                     /gene="cycA"
                     /locus_tag="ACHOQQ_RS17780"
                     /old_locus_tag="pgaptmp_003556"
                     /gene_synonym="dagA"
     CDS             1877..3289
                     /gene="cycA"
                     /locus_tag="ACHOQQ_RS17780"
                     /old_locus_tag="pgaptmp_003556"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGU4471765.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3334..3996)
                     /gene="ytfE"
                     /locus_tag="ACHOQQ_RS17785"
                     /old_locus_tag="pgaptmp_003557"
     CDS             complement(3334..3996)
                     /gene="ytfE"
                     /locus_tag="ACHOQQ_RS17785"
                     /old_locus_tag="pgaptmp_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGU4471766.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4104..5069)
                     /gene="ytfF"
                     /locus_tag="ACHOQQ_RS17790"
                     /old_locus_tag="pgaptmp_003558"
     CDS             complement(4104..5069)
                     /gene="ytfF"
                     /locus_tag="ACHOQQ_RS17790"
                     /old_locus_tag="pgaptmp_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4471767.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGPLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5177..6037)
                     /gene="qorB"
                     /locus_tag="ACHOQQ_RS17795"
                     /old_locus_tag="pgaptmp_003559"
     CDS             complement(5177..6037)
                     /gene="qorB"
                     /locus_tag="ACHOQQ_RS17795"
                     /old_locus_tag="pgaptmp_003559"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4471768.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHRFNVNN"
     gene            6126..6506
                     /gene="ytfH"
                     /locus_tag="ACHOQQ_RS17800"
                     /old_locus_tag="pgaptmp_003560"
     CDS             6126..6506
                     /gene="ytfH"
                     /locus_tag="ACHOQQ_RS17800"
                     /old_locus_tag="pgaptmp_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGU4471769.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6624..8567)
                     /gene="cpdB"
                     /locus_tag="ACHOQQ_RS17805"
                     /old_locus_tag="pgaptmp_003561"
     CDS             complement(6624..8567)
                     /gene="cpdB"
                     /locus_tag="ACHOQQ_RS17805"
                     /old_locus_tag="pgaptmp_003561"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGU4471770.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8757..9497
                     /gene="cysQ"
                     /locus_tag="ACHOQQ_RS17810"
                     /old_locus_tag="pgaptmp_003562"
     CDS             8757..9497
                     /gene="cysQ"
                     /locus_tag="ACHOQQ_RS17810"
                     /old_locus_tag="pgaptmp_003562"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGU4471771.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2168 a         2444 c         2514 g         2414 t
ORIGIN      
        1 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
       61 tccttagctg gcttctggtt gacccttcat ctaccgcgcg cgaataaaac tgccatccgg
      121 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ctgtcaccac
      181 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggttt
      241 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      301 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      361 tccgctatct ggctggaatg gttgtgtttg ggtagtttgc ggctgctctt ctggctggcc
      421 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      481 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgtgactgaa tatctaactg
      541 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      601 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      661 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      721 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
      781 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
      841 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
      901 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
      961 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1021 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1081 cgctggaagg caaacatccg gctgttccgg ttgatgtggt gcatcgcgca ctgcgtgaaa
     1141 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1201 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1261 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1321 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1381 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1441 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgtg
     1501 gcgcaggcgc gtccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1561 tcctctaagc agcgcattct gttcccctcg agccagaggg gagcaggcat tcagcaataa
     1621 ccccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1681 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1741 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     1801 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     1861 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     1921 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     1981 tgccattggt acggggttgt ttatggggtc tggcaaaacg attagccttg ccgggccgtc
     2041 gatcattttc gtttatatga tcattggttt tatgctcttt ttcgtgatgc gggcaatggg
     2101 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2161 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2221 tatggcagac gtggtggcga tcacggctta tgctcagttc tggttccccg atctctccga
     2281 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg cttaacctcg ccaccgtgaa
     2341 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2401 gattgtcgtc ggcctggtca tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2461 gtcattcgcg catttgtgga atgacggcgg ctggttcccg aaaggtttaa gtggcttctt
     2521 tgccggattc cagatagcgg ttttcgcttt cgtggggatt gagctggtag gtacaacagc
     2581 tgcggaaacc aaagatccag agaaatcact gccacgcgcg attaactcca ttccgatccg
     2641 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2701 ggtagtcccg gagaaaagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgctgc
     2761 cgcaagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     2821 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     2881 cgctaaactt tctaagcgcg cagtaccagc gaaagggctg acgttctcgt gtatctgtct
     2941 gctcggcggc gtggtgatgc tgtatgtgaa tcccagcgtg attggcgcgt tcacgatgat
     3001 cacgaccgtt tccgcgattc tgtttatgtt cgtctggacg attatccttt gctcgtacct
     3061 tgtgtatcgc aaacagcgtc ctcatctaca tgagaagtcg atctacaaga tgccgctcgg
     3121 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3181 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cgctggggct
     3241 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3301 tgctggatgg cgcaatgcca tccagctttt agatcactca cccgccagcg cgcgtgggaa
     3361 cagcacatta ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatgcc
     3421 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtatt
     3481 gtgtttgatc acttccagca gttcacccgc ttcatcgtgc tcgctttcca ttacgctgat
     3541 tggccccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3601 tttcatcatg tggctggaaa gctcttcatg cagcatggtc aggtattttg tcagcccttt
     3661 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca gaatcagctc
     3721 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcga tgatttctgc
     3781 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagctcagc
     3841 ttcaatgacc tcaacatcca gttctttacg tgccgccgca cgcgccagcg tttgcttacc
     3901 gccgcagcag taatccatat catatttacg aaacagagcc gaagcgcgag gaatagagag
     3961 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataag
     4021 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4081 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4141 ggttttgctc tgaccgcgat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4201 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4261 aaaacaatca gcggcccgag aatcacggtc ggtaatcgct ggctggcgac gttccagcag
     4321 agtgcgccga cccatgagca aagcacggct atcgcaatca tcagactaat aaacaccagc
     4381 ggacgggggc caaagggtag ggagaagtcc ggcgtttgcg tattcagcca gtaacaagcg
     4441 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4501 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4561 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4621 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4681 atcagtgccg gggcgagttt tccccacgcg agtttgccgt cgcgctggct ataaagcaga
     4741 ttggcaaaga caggaatgac caccggcagg gggccgataa tcatcgtgga aacaggcgcg
     4801 ccagtacgtt gaatggcact ggcaaggcag aagtaataaa tgaggttgcc catcatagtg
     4861 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     4921 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     4981 gccgggtatt ccggcacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5041 aacagggcgt acagcacgcc gctaatcatt actggcatcc attgatctgt cagaagaaag
     5101 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5161 atgccacttt aattaactag ttattaacat taaaacgatg gcttacgctt tcggctaacg
     5221 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gtcatcaaat agaccgcctt
     5281 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5341 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5401 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5461 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5521 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5581 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5641 tgtaaacgat gccagaatca gccaacattt tctccgtctc aatgtgctca tcggcgaggc
     5701 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     5761 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     5821 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     5881 agtcagcctg acgcacggta atgccttgtg ctgccagggc ctgggctttt gccggattac
     5941 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaagattca ataacatagt
     6001 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6061 tgagtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6121 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6181 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6241 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatg ggcggggtga
     6301 gtgaaaagat gcttgcgcag tcgttacagg cgctagagca ggatggtttt cttaaccgta
     6361 tcgcgtatcc ggtggtgccg ccgcatgtgg aatatagcct cacgccgctg ggcgagcagg
     6421 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttag
     6481 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6541 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6601 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6661 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6721 gctttatctg acggggaagt ttcgaaacgg atatccagtt tcttatcgcc agctatcggt
     6781 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     6841 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     6901 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     6961 atagcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7021 gcattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7081 ttcacaccat caataacatc aaagttatag gtgcggaaac cgtcccagtt gatgagtgac
     7141 tgtggtttcg tgctgttggg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7201 tctttcacct ctttaccgct ggctttcacc acaatcagcg tattgggata gagataaaga
     7261 tcggcggcat tacggaaagt cagctgacct ttttccacct ctacatagct tgccgggtca
     7321 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7381 tccggatcgc cctgaatgta atgctcgaca tacgcttttt gcgcgttgtt caccacctgc
     7441 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7501 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7561 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgtgcttcc
     7621 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7681 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7741 gcgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatgg
     7801 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     7861 atcactttat acggatcggc agatagcccg gaatgcgcca gaacgacaac gacatcggca
     7921 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     7981 actttcccgg ataaattagc tttatcccag cccatgattt gtggcggcac gacgccaata
     8041 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt gtctttaatt
     8101 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8161 gggaatttcg ctcctgccag cgcatttttc aggtaatcca gaccgtagtt aaactcgtgg
     8221 ttgccaagcg ttccgacggt atagtccagc gtatttaatg ccttatagac cgggtgaata
     8281 tcgcctgctt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaag
     8341 tcgccgttat caaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8401 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8461 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8521 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8581 ttatcatcgg gaatacgaaa gaaaagggag aataaacgcc ttacttatag aacagtgaag
     8641 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8701 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     8761 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     8821 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     8881 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     8941 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9001 tagacccact ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9061 ttgcgctcat tgatcatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9121 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9181 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9241 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9301 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9361 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9421 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9481 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt ctgccatcct
//
LOCUS       DAZKFU020000182         9477 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZKFU020000182 DAZKFU020000000
VERSION     DAZKFU020000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.184"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            114..1178
                     /gene="ulaG"
                     /locus_tag="ACHOQQ_RS17815"
                     /old_locus_tag="pgaptmp_003563"
     CDS             114..1178
                     /gene="ulaG"
                     /locus_tag="ACHOQQ_RS17815"
                     /old_locus_tag="pgaptmp_003563"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGU4471772.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1286..2041
                     /gene="ulaR"
                     /locus_tag="ACHOQQ_RS17820"
                     /old_locus_tag="pgaptmp_003564"
     CDS             1286..2041
                     /gene="ulaR"
                     /locus_tag="ACHOQQ_RS17820"
                     /old_locus_tag="pgaptmp_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGU4471773.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2038..2787)
                     /gene="yjfP"
                     /locus_tag="ACHOQQ_RS17825"
                     /old_locus_tag="pgaptmp_003565"
     CDS             complement(2038..2787)
                     /gene="yjfP"
                     /locus_tag="ACHOQQ_RS17825"
                     /old_locus_tag="pgaptmp_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4471774.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2969..3298
                     /gene="bsmA"
                     /locus_tag="ACHOQQ_RS17830"
                     /old_locus_tag="pgaptmp_003566"
     CDS             2969..3298
                     /gene="bsmA"
                     /locus_tag="ACHOQQ_RS17830"
                     /old_locus_tag="pgaptmp_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGU4471775.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3447..3722
                     /gene="yjfN"
                     /locus_tag="ACHOQQ_RS17835"
                     /old_locus_tag="pgaptmp_003567"
     CDS             3447..3722
                     /gene="yjfN"
                     /locus_tag="ACHOQQ_RS17835"
                     /old_locus_tag="pgaptmp_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGU4471776.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3839..5464)
                     /locus_tag="ACHOQQ_RS17840"
                     /old_locus_tag="pgaptmp_003568"
     CDS             complement(3839..5464)
                     /locus_tag="ACHOQQ_RS17840"
                     /old_locus_tag="pgaptmp_003568"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGU4471777.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5548..6711)
                     /locus_tag="ACHOQQ_RS17845"
                     /old_locus_tag="pgaptmp_003569"
     CDS             complement(5548..6711)
                     /locus_tag="ACHOQQ_RS17845"
                     /old_locus_tag="pgaptmp_003569"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4471778.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6714..7352)
                     /locus_tag="ACHOQQ_RS17850"
                     /old_locus_tag="pgaptmp_003570"
     CDS             complement(6714..7352)
                     /locus_tag="ACHOQQ_RS17850"
                     /old_locus_tag="pgaptmp_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGU4471779.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7362..7760)
                     /locus_tag="ACHOQQ_RS17855"
                     /old_locus_tag="pgaptmp_003571"
     CDS             complement(7362..7760)
                     /locus_tag="ACHOQQ_RS17855"
                     /old_locus_tag="pgaptmp_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGU4471780.1"
                     /translation="MHILDSLLAFSAYFFIGVVMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7778..8437)
                     /locus_tag="ACHOQQ_RS17860"
                     /old_locus_tag="pgaptmp_003572"
     CDS             complement(7778..8437)
                     /locus_tag="ACHOQQ_RS17860"
                     /old_locus_tag="pgaptmp_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGU4471781.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8488..9186)
                     /locus_tag="ACHOQQ_RS17865"
                     /old_locus_tag="pgaptmp_003573"
     CDS             complement(8488..9186)
                     /locus_tag="ACHOQQ_RS17865"
                     /old_locus_tag="pgaptmp_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGU4471782.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9205..>9477)
                     /locus_tag="ACHOQQ_RS17870"
                     /old_locus_tag="pgaptmp_003574"
     CDS             complement(9205..>9477)
                     /locus_tag="ACHOQQ_RS17870"
                     /old_locus_tag="pgaptmp_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGU4471783.1"
                     /translation="LFTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGIS
                     SVQQEEYYIVFGALSLKSSLEDILLEIASLVDNALDLAEITEEYSH"
BASE COUNT         2458 a         2507 c         2268 g         2244 t
ORIGIN      
        1 cacccggcaa aaagtcaaaa aatggtcatt gccttcctga aaaaatagtg tcaggattaa
       61 tcaaaaccaa tcatatgttg attcgaattg aactatcatg aaggtaaatg gcgatgagta
      121 aagtgaaaag tatcacccgt gaatcctgga tcctgagcac tttcccggag tggggtagct
      181 ggttgaatga agaaattgaa caagagcagg tagctcctgg tacatttgcg atgtggtggc
      241 ttggctgcac cgggatctgg ctgaaatcgg aaggtggcac caacgtttgc gttgatttct
      301 ggtgcggcac aggcaaacaa agtcacggta acccgttaat gaaacagggt caccagatgc
      361 agcgtatggc tggcgtgaaa aaactgcagc cgaacctgcg caccaccccg tttgttcttg
      421 atccatttgc tattcgccag atcgacgccg tcctggcgac tcacgatcac aacgatcata
      481 tcgacgttaa cgtcgctgct gccgtgatgc agaactgtgc tgatgacgta ccgtttatcg
      541 gaccgaaaac ctgtgtggat ttatggatcg gctggggcgt gccgaaagaa cgttgcatcg
      601 tggttaaacc gggcgacgtg gtgaaagtca aagacattga aattcatgcg cttgatgctt
      661 tcgaccgtac tgcactgatc accctgcctg ccgatcaaaa agcggctggc gtactgccag
      721 atggcatgga cgatcgcgcg gtgaactacc tgttcaaaac gcctggcggc tccctgtatc
      781 acagcggcga ctcccactac tctaactatt atgcgaagca cggtaacgaa catcagatcg
      841 acgtggcgtt aggatcgtac ggcgaaaacc cgcgcggtat caccgacaaa atgaccagcg
      901 ccgatatgct gcgtatgggc gaagcgctga atgcgaaagt agtgatcccg ttccaccacg
      961 atatctggtc aaacttccag gccgatccgc aagagatccg cgtgctgtgg gagatgaaaa
     1021 aagatcgcct gaaatatggc tttaagccgt ttatctggca ggtgggcggc aaatttacct
     1081 ggccgctgga taaagacaac ttcgagtacc actatccgcg cggtttcgat gattgcttca
     1141 ctattgaacc ggatctgccg ttcaagtcat tcctgtaatc tgtcgtttat accggatagc
     1201 gtggagaagt tcgcgctatc cggcgtagta tttcctggct atttcaaata tcatctaaaa
     1261 aaatcaaatt ttatcggaat agctcatgac tgaagcacaa agacatcaaa tcctcctgga
     1321 aatgctcgca caattgggct ttgtgaccgt tgagaaagtc gttgagcgtc tgggaatttc
     1381 gcctgccact gcgcgacgcg atatcaataa acttgacgaa agcggcaaac tgaaaaaagt
     1441 gcgcaatggc gcagaagcta ttacccaaca gcgcccgcgc tggacgccga tgaatctgca
     1501 tcaggcgcag aatcacgatg aaaaagtacg tattgctaaa gcggcctcgc agctggttaa
     1561 tccgggcgaa agcgtagtca tcaactgcgg ctccaccgcg tttctgcttg ggcgggaaat
     1621 gtgtggcaag ccagtgcaaa tcatcactaa ttatctaccg ctggcaaatt acctgatcga
     1681 tcaagaacat gacagcgtga tcattatggg cggacagtac aacaaaagtc agtccatcac
     1741 tttaagcccg cagggcagcg aaaacagtct ctatgccggg cactggatgt ttaccagcgg
     1801 aaaagggctg accgcagaag ggttgtataa aaccgatatg ctgacagcaa tggcagagca
     1861 gaagatgctg agcgtggtag ggaaactggt ggtactggtt gatagcagta agattggcga
     1921 acgcgcggga atgcttttta gccgtgccga tcaaatcgat atgcttatca ccggcaaaaa
     1981 tgctaacccg gaaatcctgc aacaactgga agcgcaaggg gtcagcattc tgcgtgttta
     2041 aagatgctgg cggaaaaatg tcacggcagc atctaacgcc tcaggcgtaa tgcggtgacg
     2101 cacgcctggc tgccatgaac aggttagcag tttatcccgt cccgtctcgc ttaaggcctg
     2161 ctgcaaacgt agtgattcat cggcaggcac aacgtcatcg tcgaggccat gccacagcag
     2221 tagaggtctg tccccaagtt gttccaggtg gtttgtcgct tcccactctg ccagcggcgc
     2281 gacaatgtta ttgaattcat tctgctgtgc tgccgtttca ggtatcagcg gtggaaacag
     2341 tgaacgggcg agcgatgtaa aatagcccga tcccatcatg ctggcggtac atttcaccgt
     2401 ggggtggcga gcggtaattc ccagtgccgt catcgcgccc atcgacgcgc caccgactgc
     2461 cagacggtca tcaagcagcc agttttcttc ggctattgcc gcacgtaaag tagtgaattc
     2521 ctgcatactt tgtagcaaga tttgccagaa ttgatttaac cgccgcgctg cgtcgccact
     2581 aaaacgacta ccgtgatcgg gcgcatccgg catgatcacc cgcaaaccag cctgcgccag
     2641 cgcaacggca aaatagctat acaccagact ggacgaagta aagccgtgat aaaaaatcac
     2701 gcacggtaac ggggtatctt tttgtccgac aggataagca tgaagaacgg gaatatctgc
     2761 cagctcgcgt gattctattt caatcatctg tctctccttt ttgtctggta tgacatgaag
     2821 aaatccttag cacaagtttc tgctggcggt attagttcat aatgttgaga tgtgggttac
     2881 gctttcgtca cgttttcatt cgaaaatcgc gtaccaggta acaattcggg aacattcccc
     2941 caaagttaaa ttaataaggc actacactat ggttagcagg aaacgtaata gcgttattta
     3001 ccggtttgcc agtttattat tggtgttgat gttaagtgcc tgtagcgcac tgcaaggtac
     3061 gccacagcca gcaccgccag taacggacca tccgcaagag attcgccgcg accagacgca
     3121 agggttacag cgaataggta gcgtaagcac aatggttcgg ggttctccgg atgacgcatt
     3181 agcagaaatc agagcaaaag ctgtcgctgc aaaagctgat tattacgttg tcgtcatggt
     3241 ggacgaaacc atcgtgacag gacagtggta ttcacaagcc attttgtacc gtaaataaat
     3301 cagacaactt acatagtcag ctttacattg ctttgcgtcc ctatgcgttt acctgtgcac
     3361 aataaattac cggctggaag cctgggcgtt tattaccgcg atggaatgcc ctgcaacatg
     3421 tggggaaacg aaaaatggag ctgacgatga aacaattact tgcctcaccc tcgctgcaat
     3481 tagtgactta tcctgcgagc gccacggcac agtctgcaga attcgccagt gctgactgcg
     3541 tgacgggcct gaatgaaata ggtcagatct cagttagcaa tatttcaggg gatccgcagg
     3601 acgtggaacg tatcgtggcg ttaaaggccg atgaacaagg tgcgtcatgg taccgcatta
     3661 ttaccatgta cgaagaccag cagcctgata actggcgcgt acaggcgata ctgtatgcat
     3721 gagtgtcatc tacagcaaat gaacaatgcc ggatgcggcg cgagcgcctt gtccggccta
     3781 caattttcac atatttcatt gagttaatcg aaaccagcgt cgcatcagtc gtatacgctt
     3841 acacacacac tccccccgtc gcccgcagca ataaatcatt ctggatctgc tctgacaacc
     3901 gtacgccgcc gcgcgtatct aacatcacct gacaccacgc ctgcgccatt ggcggagagg
     3961 catatttcaa catttgcgca ccgcagccca gcaggaatag ctgatgagta atctctcgcc
     4021 ccagttcttc agctggctta cgcagctgct gctgtaaacg acgaaccgcg cgatcaaaat
     4081 agcgatcctg ccctttcact tccacaaatg cttccgacaa taagtcgtat acgcccgctt
     4141 gcttattgag aacgcgcagc acatccaggc acataatatt gccggaacct tcccaaatac
     4201 tgtttaccgg catctcccgg taaagccgcg gcagctcgct ctcctcgcaa taaccaatgc
     4261 cgcccagcac ctccatcgct tcggccacaa acggcatacc tcgtttgcag atcacaaatt
     4321 ttgccgcagg cgtaaataaa cgcgcccaca gggcttcttt ggcatcggct cgccggtccc
     4381 acgctcgcgc aagacgaaac agcaacgccg tttgcccttc aagctgaagt gccatgcgac
     4441 ttaagacatg acgcataagg ggctgttgga tcaatggatt accaaaaaca tggcgttgat
     4501 gcgcatgata aatcgccagc gagaacgcac ggcgcatcat ggcatggcta cccagggcgc
     4561 aatcaaaacg cgtcatcccc cccattttca ggatcagacg aattccttcc ccttcctgcc
     4621 ccaataacca gccaatggca tcctgaaact ccacttcaca actggcgtta gagcgattac
     4681 ccagcttatc tttcagccgc tcgaggcgaa tcgcgttgcg ttgcccgtca ggcaaaaagc
     4741 gcggcacaaa aaagcaggac aaaccgcccg cggtctgcgc cagcaccaga tgcgcatcgc
     4801 tttgcggaac cgagaaaaac catttatgcc ccaccagccg ataagagcca tcctccagac
     4861 gctctgcgcg ggtggtgttg ctcatgacat cggaaccgcc ctgcttttcc gtcattccca
     4921 tgccaatcaa caaaccgcgt ttttgcccac ctggcaataa gtgagaatcg tagcgatcgc
     4981 tcaacagcgg cgtggtccag tcctgaaacg gcgtgggtaa catctgtaac aacaatggcg
     5041 tggcggcaaa ggtcatcgtt attggacata acgaccccgc ctcaacctgt gcgtgcaaca
     5101 taaaacgcgc cgcgcgcgcc acaaatgcgc cggagcgagc gtcttcttcc caggcaagat
     5161 tgtgcacccg attggtacat agcgcctgca tcagcaggtg ccaggcgggg tgaaaacgca
     5221 catcgtccag acggcgtcct tgcgcatcgt agcgcaataa ttcaggcgga ttcacattcg
     5281 ccagccgccc cagttcaagg gattcagccg ttcctaactg ctgaccaata ctggctagaa
     5341 aatcgctatc ccagccagca ccttcacgcg ttaccgcttc gcaaagcgcg ccatcagaca
     5401 ggtataagtt gctgttattt aatggtatag gttgattaaa aacggtgtga gtttgccagt
     5461 gcactgtgtc tccctccatc aatggcagtc accattagta tggtcactgc catggattca
     5521 tgacagaaag agattgctaa aacattctta tccagcaatg taatgcggaa cgaaacgtga
     5581 ggtgtctttg gtgatcagtg tgttatcttc acgaatcccc attccgcacg cttcatcatc
     5641 gacaatccag ctaccgatga gtgtgtagct atcgccaaac cgcggcagag gttgaaacgc
     5701 ctggtagatc atcggttcat cggcgtaatc accatcagcg tggtcaacga cgttattctg
     5761 gccgtcgaaa atggtgacat ttccgccttc gcgcgagtag atcggtttac gcacatagct
     5821 ttcgccagcg gcaatctgcg gtttttcacc ctcaaaccag gatgcaagaa ggttaggatg
     5881 accagggaag aagcgccaaa gcagcggcat tagcccttta ttactcaaga tacttttcca
     5941 taacggctcc acccattgct cgcgacgctt gcgcagcagc ggaccgttat cgtcacgcat
     6001 catccactcc agcggataca gcttaaatgc acgctggatg acattatcat caagatcggt
     6061 cagtacgccg ccgacgccca aaccgagatc ttcaatgtag ataaaccgcg actcctgccc
     6121 tgcctgctgg gcgcagtcct gcaagtacag cacggtagtc ctgtcttcat cggtgtcctg
     6181 acagcagcag aaataaaacg gctcccgact gtaaagctca ctaaagcgcg aaatcaggcg
     6241 ttcctgaatg gcattgtact gatcggcatc acgcggaata atgccgctgc gccgggcatc
     6301 ctctagccac aaccactgga aataagccga ctcatacaac gaggtcggcg tatcggcgtt
     6361 gtactccagc agcttcaccg gcgcattgcc acaccaggca aaatccatcc ggccatacag
     6421 cgaaggatcg cgtgcgcgcc agctttcagc gatcacatcc cagtacaacg gagggatcgc
     6481 cagttgcgtc aggatctctt cgtctttaac cgcgcgatcc accacctcaa ggcacatctg
     6541 atgcagttcc gcagtcggtt tttcgatctg ctcttcaatc tggcgcagag taaaacggta
     6601 agcccgactc tcgtcccaat agatttcatt gtcgatgata tgaaagtcga aaccgttgtc
     6661 agcggcgatt tggtccagat cccgtcgcac aggaacgttg tgtctcagca tgattagcct
     6721 ccccaatgcc cacgggcgct ggaagaacga ccatagccgc cgcgagaaac ggttgacgct
     6781 tttttggtgg taaagccgcc tgaagagtaa gactcttttt tgccagaacc ggagcgccag
     6841 gagtaatcac cagaagtgtt gcgccagacg gggcgggaag caaatgagga tccgccgctg
     6901 ttataaacaa actgctcatc gcgatcttta cgaataaccc ggcttaacaa aaatccagaa
     6961 acgaccggga tccaactctg ttcaacgttg tcgtagtagc aattttcgta cttagactga
     7021 caattctgct gagtcatatt cttcggaaca tcggcataaa atgctgcttt ggcgttattc
     7081 cagccacgcg cgcaaatgtc tgcattatta ccgtcatcaa tacaatcctg cactgtcgcg
     7141 taaaacgttc cgtcgccgtc gttatcgaca tcgccactat cgccacaacc tttcaataca
     7201 aaaaaagcgg caccgcccat tagcgccaac gttaagtatt tttgtgcgta gcgattgcga
     7261 cgcggttcaa aaggattatt cggtctacca atgcgactga tcgctccgtg accaatttta
     7321 ctgttatttc ttgatttgcg ttttctggcc atgatcgtcc attaccatgt catacaagct
     7381 gcgttaaaaa taccgccagc cagcgcggcg gttcccataa acattcctgc tgcggtattg
     7441 tgattaataa ttttttcgct taatgcgggc atataaagtc tgacaccagc aaaaacgagt
     7501 aactgaatca ccagtgcaat cccgccccag gcgaaatagt ctggaatact caccgcattg
     7561 attgccgcac tggataaggg gatcacgtaa cccaacaatg taccgctgaa cgccagtgac
     7621 gctgcggtat tattgttttt gattaattgc cattcattgt gcggtgtaat tttggagtag
     7681 ataaacagaa aaataatcac catcaccacg ccaataaaaa aataggcact aaaggcaaga
     7741 agtgaatcca gtatatgcat tgtaaacgtc cttgtaatta accaataata tgaagtgatg
     7801 tcagcgggat atcgacaccc aacgcgcgcg aaaataacca ctctggctcg ccgagatcgt
     7861 taaaagattc ctcaccattt agcagcaaat attcgtaagt atcttcggtc acttgccgct
     7921 ggtagcccat ggtaaaatta tggacttccc atttagcatg attttgattt tctacttttt
     7981 ccagcatgta gacgggttcg atatttcccg gttcttcgct gttaaaaaat ctctgccagc
     8041 gtttttcctg ccagtttaag gtcattgccc ccatcgtttt ggcgttgatc gcctcgcgcc
     8101 agtgactctc tttactgata ccgtagctct cttcatagac aaagagcttg atatcatcaa
     8161 tgtcatcaat atcttcgccg ccggtggtat tgatttgcag aaactcatcg cctgaagtgt
     8221 agtagcggaa aatctgacta ccgccgccca gatcgatgtg gctgacagcg gctaccgtaa
     8281 attcctcacc cggcagcgca atcagcaatt catcttccag caaacgaaac gctaacgtgt
     8341 cgagcgtaaa gccactattg agatgaagtc ccagcggacc gcgagcgata gcaggcttat
     8401 tatccttgcc aaacagacgc tggaaaaaac cagacatatt ttatccttaa aatagttgcc
     8461 agccttgtag tgaggctggc aaaaaaatta ctcgccctgt tggcgttgca gtcttgctaa
     8521 tacatcctgg gcgctacttt tattgctacc gccaattccg gcttccgcca gcttttcgtc
     8581 aaggtcgcga ccgtctgcga ctttctccaa ctgtgcggca gcatccaggc gagcctgacg
     8641 ttctgcctga cgcgtttgca ggcgttttaa ggattctgcc gctgtcgaaa cgctggaaga
     8701 tgcgccaacg gtagaggttg ttacagcctg ttgtgcacgc tgcatggctt cagtggcttt
     8761 aacgacttcc atttgctgct caaactgagc gatacgctgc gctgtcgctg taactgcttt
     8821 ttccacgcca tcacgggaga cttccagatt cgacaacact tgctcttcag cggtaatgag
     8881 attctcaagg cgcgcgattt cttcagcaac ttcgttaatc aacgacggat taacgttctt
     8941 gcttaacgct tccagcgcac gggcttccag actggctttg cgctcacgta aatctttcag
     9001 cttatcgtga cttaatttca cccgcgccag cagatcgacg cgagattttc cggctttatc
     9061 gagttcagct ttagcgtcac gaatatgctg ttccagcatg cgcacgccct gggtttcttc
     9121 aatggactct tccgcctggg agataaacga tttccccagc gtgaataaac tttttaaaat
     9181 tcccataaat acccgtcctt gtaattagtg tgaatattct tctgtaattt cagccagatc
     9241 caatgcgtta tctaccagcg aggctatctc aagcaggata tcttcaagag aagatttaag
     9301 cgataacgcc ccgaaaacaa tgtaatactc ttcctgttgc acgctggaga ttcctaccga
     9361 tgacaacggc atcatcttct gatttcttaa taagaaggta ttaaattcat ctggattgct
     9421 gatactactt accggacaaa taaaggtttc gataatcatt tggcgggaag taaaaag
//
LOCUS       DAZKFU020000183         9397 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZKFU020000183 DAZKFU020000000
VERSION     DAZKFU020000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.185"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2875
                     /gene="evgS"
                     /locus_tag="ACHOQQ_RS17875"
                     /old_locus_tag="pgaptmp_003575"
     CDS             <1..2875
                     /gene="evgS"
                     /locus_tag="ACHOQQ_RS17875"
                     /old_locus_tag="pgaptmp_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HGU4471784.1"
                     /translation="NFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWLDTGNLAFL
                     NKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNIITLQTGLN
                     FSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVFVMQKAPDS
                     EQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELDALVATQLN
                     SRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTE
                     KWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQ
                     ISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADFYKNAMLPLENSDSPFKDVF
                     SNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYICGWQDITE
                     TRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGSGLSKEQRV
                     EAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSFGAIAASK
                     SIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLVHIDDNHAV
                     IKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLES
                     HPGIGTTFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRLLLKRQLNL
                     LGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSSLPIWGLTA
                     NAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVVHIAPQYRHLDIEALKNNTA
                     NDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANILNLQKLINI
                     SHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(2931..4076)
                     /gene="yfdE"
                     /locus_tag="ACHOQQ_RS17880"
                     /old_locus_tag="pgaptmp_003576"
     CDS             complement(2931..4076)
                     /gene="yfdE"
                     /locus_tag="ACHOQQ_RS17880"
                     /old_locus_tag="pgaptmp_003576"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HGU4471785.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4150..5094)
                     /gene="yfdV"
                     /locus_tag="ACHOQQ_RS17885"
                     /old_locus_tag="pgaptmp_003577"
     CDS             complement(4150..5094)
                     /gene="yfdV"
                     /locus_tag="ACHOQQ_RS17885"
                     /old_locus_tag="pgaptmp_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGU4471786.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5164..6858)
                     /gene="oxc"
                     /locus_tag="ACHOQQ_RS17890"
                     /old_locus_tag="pgaptmp_003578"
     CDS             complement(5164..6858)
                     /gene="oxc"
                     /locus_tag="ACHOQQ_RS17890"
                     /old_locus_tag="pgaptmp_003578"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGU4471787.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6912..8162)
                     /gene="frc"
                     /locus_tag="ACHOQQ_RS17895"
                     /old_locus_tag="pgaptmp_003579"
     CDS             complement(6912..8162)
                     /gene="frc"
                     /locus_tag="ACHOQQ_RS17895"
                     /old_locus_tag="pgaptmp_003579"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGU4471788.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISIDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSDFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(8674..9288)
                     /locus_tag="ACHOQQ_RS17900"
                     /old_locus_tag="pgaptmp_003580"
     CDS             complement(8674..9288)
                     /locus_tag="ACHOQQ_RS17900"
                     /old_locus_tag="pgaptmp_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGU4471789.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQKTAAAERISE
                     QGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNVNDDQ
                     YIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQYLMP
                     LKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         2714 a         2160 c         1953 g         2570 t
ORIGIN      
        1 taattttttc ttgaccagaa aagaatctgt cgttcttaat gaagtactca atcgatttgt
       61 tgatgctttg acaaatgaag ttcgctatga agtatcacaa aattggcttg atacaggaaa
      121 cctggccttt ctgaacaaac cattagaact cactgaacat gaaaaacaat ggattaagca
      181 gcatcccgat ttaaaggtgc tggaaaatcc ttactcgccc ccctattcta tgacggatga
      241 aaatggctcg gttcggggcg ttatggggga cattcttaat attattacct tgcaaacagg
      301 tttaaatttt tctccgatca ccgtttcaca caatatccat gctggaacac agctgaatcc
      361 cggcggatgg gatatattac ccggtgctat ttatagtgaa gatagagaaa ataatgtttt
      421 atttgctgaa gccttcataa caacgcctta cgtttttgtc atgcaaaaag cgcctgacag
      481 tgaacaaaca ttaaaaaaag gaatgaaagt tgccattcca tattattatg agcttcattc
      541 gcaattaaaa gagatgtatc cggaggtaga gtggataaaa gtcgataacg ccagcgctgc
      601 atttcacaag gtcaaggaag gcgaacttga tgctctggtc gcgacacagt taaattcacg
      661 ttacatgatc gaccattact atcctaatga actttatcat tttcttattc ccggcgttca
      721 gaatgcatca ctttcgttcg cttttcctcg cggagaaccg gaacttaagg atattattaa
      781 taaagcactg aatgcaatcc ccccaagcga agttctgcgc ctgacggaaa aatggattaa
      841 aatgcccaat gtgaccattg acacatggga cctctatagc gagcaatttt atattgttac
      901 gacattatcc gttttattag ttggcagtag ccttttatgg ggattctacc tgttacgctc
      961 agttcgtcgt cgtaaagtca tccagggtga tttagaaaac caaatatcat tccggaaagc
     1021 actctcggat tccttaccga atccaactta tgttgtaaac tggcaaggta atgtcattag
     1081 tcataatagt gcttttgaac attatttcac tgcggatttc tataaaaatg caatgttacc
     1141 attagaaaac agtgactcac cctttaaaga tgttttttct aatgcgcatg aagtcacagc
     1201 agaaacgaaa gaaaatcgaa caatatacac acaggtattt gaaattgata atggcatcga
     1261 gaaaagatgc attaatcact ggcatacatt atgcaatctg ccagccagcg atcatgctgt
     1321 ttatatttgt ggttggcaag atattaccga gacgcgtgat ttaattcatg cactcgaagt
     1381 agagagaaat aaagcgatca atgcaactgt cgcaaaaagt cagtttctgg caacaatgag
     1441 tcacgaaata agaacaccaa taagctccat tatgggcttc ctggaacttc tgtcgggttc
     1501 tggtcttagc aaggagcaac gggtggaggc gatttcacta gcctacgcca ccggacaatc
     1561 actcctcggc ttaattggtg aaatccttga tgtcgacaaa attgaatcgg gtaactatca
     1621 acttcaacca caatgggtcg atatccctac tttagtccag aacacttgtc actctttcgg
     1681 tgcgattgct gcaagcaaat cgatcgcatt aagttgcagc agtacatttc ctgatcatta
     1741 cctggttaag atcgaccctc aggcgtttaa gcaggtctta tcaaatttac tgagtaatgc
     1801 tctcaaattt accaccgagg gggcagtaaa aattacgacc tccctggttc acattgatga
     1861 caaccacgct gtaatcaaaa tgacgattat ggattctgga agtggattat cacaggaaga
     1921 acaacaacaa ctgtttaaac gttatagcca aacaagtgca ggtcgtcagc aaacaggttc
     1981 tggtttaggc ttaatgatct gcaaagaatt aattaaaaac atgcagggtg atttgtcatt
     2041 agaaagtcat ccaggcatag gaacaacatt tacgatcaca atcccggtag aaattaccca
     2101 acaagtggcg gctgtcgagg caaaagcaga acaacctatc acactacctg aaaagttgag
     2161 catattaatc gcggatgatc atccgaccaa caggctatta ctcaaacgcc agctaaatct
     2221 attaggatat gatgttgacg aagccactga tggtgtgcaa gcgctacaca aagtcagtat
     2281 gcaacattat gatctgctta ttactgacgt taatatgccg aatatggatg gttttgagtt
     2341 gactcgcaaa ctccgtgagc aaaattcgtc cttacccatc tgggggctta cagccaacgc
     2401 acaggctaac gaacgtgaaa aagggttaaa ttgcggcatg aacttatgtt tgttcaaacc
     2461 gttgaccctg gatgtactga aaacacattt aagtcagtta caccaggttg tgcatattgc
     2521 acctcagtat cgccaccttg atatcgaagc cctgaaaaat aatacggcga acgatctaca
     2581 actgatgcag gagattctca tgactttcca gcatgaaacg cataaagatc tacccgctgc
     2641 gtttcatgca ctagaagctg gcgataacag aactttccat caatgtattc atcgcatcca
     2701 cggtgcggct aacatactga atttgcaaaa gttgattaat attagccatc agttagaaat
     2761 aacacctgtt tcagatgaca gtaaacctga aattcttcag ttgctgaact ctgtaaaaga
     2821 acacattgca gagctggacc aggagattgc tgttttctgt caacaaaatg actaaatagc
     2881 ggctcccaca atgttcaaat gtgggagcta tttaccagca catcttacat ttatgatgag
     2941 aactcctggc gaatttgttc cccatgctgg tcgagcgttg ccgctcccgg cataacatgc
     3001 gggtccgcgc agccactgat ttttatcgga ttacccggca tcattattcc cccggcttca
     3061 atcaacatat ttctcgcctg agtttgtggc aaattaatgg cctcagccac acttaataac
     3121 ggcgcgacgg gcacaccaac ttcatgtatt ctggctaacc aaacttcagc tgcctgtgtt
     3181 tttaacgtcc gctcaatata ttgtttaaga atagcctggt tttgtacgcg taaaatattg
     3241 ctgctaaatc tgggatcatt aaccagttcc gtaagctcca gtgcctggca taacgcagag
     3301 aaaagcttgt cattaccaca acaaatcgta atcggcttat cctgagtatc gaaaacatca
     3361 aaaggtgcca tgtaggggtg gcgatttccc aaacgttgtg gtgacttccc agtcgcgata
     3421 tatgccatca gcccgtgctc cagaaaactc agcgtggcat caaacatcgc tatatcgaca
     3481 tgcgcccctc tctggctctt ttcgcggcca taaagtgcac tcactattcc actgaataaa
     3541 tagacaccgc cacatagatc cgcaagagaa gtgccaacgc gcactggcgg agcatcaggg
     3601 tatcccgttt ccatcattat cccgctcatt gcctgaatga tggtatcgta ggcaggagca
     3661 tcttttagcg gaccggtatg tccgaaacct gacgatgaag catatatgag gcgcgggttg
     3721 atttcttgta acctttccca tgaaaacccc agtttttcca ttgtacctgg gcgaaaattc
     3781 tcagctaata catcagcttg tttaagcata tttataaata tacttttatc gtgatcattc
     3841 tttaaatcaa gaaccacact ctctttacca tgattaataa aactgtaata gagtgactgt
     3901 ccatccacat agggaccaaa tgtgcgggta tcatcaccat gacctggtgg ctcaacttta
     3961 attacccttg cgcccatatt acaaagaagt tgagttccga aagggccatt aaggacatgt
     4021 gtcatatcga taactaataa gccttcaaac ggccctttgc tttcattatt tgtcatttgc
     4081 ttatgccttg aaaaaagccg aaggtgaaaa gacacttcgg catttatgta aaatcaataa
     4141 catgattaat tatgaaacca gtcgactgac ataaatccac aagggagccg tgacgacaaa
     4201 acccagtacg ctcacagcca atgacgctgt accagtgcgg gtgtagacat taaaccggct
     4261 ggcaataatg attccggaga atgccggcgg taatgcgcct gccagtacca tcatctgcag
     4321 atgttcgctg ttcaaatgac atgccatacc aacaagaagc agcgccagtg gcatcagaat
     4381 cagcttcagg aaggtgttat aagcaatttc ggcactgaac tcgaatttat gtgcagccag
     4441 agtcaacccc gcagcgaata ccgctacccc tgagttagcc ttcgcaatca gattaaaggt
     4501 tgggtcccat gctgccggaa tttttacccc aaccagcacc aggatcgttg ccagaacagg
     4561 tgcccatact acaggctcct ttgccgcaga aattaatgcg ctcagattac tattcttctt
     4621 accatccgct cctgaggaag gattcagcaa atacagacca ataggaatag taattgcgtt
     4681 aacaataata gaaataattg ctaccactaa acctgttgat acggaatcac cataaatagg
     4741 atcgagaact gcaaatccca ggaatccaat ggtaggtgaa cctgcaatta atgcacatac
     4801 agctgcttct gcatgggtac gtttaaaaaa tttgtagcaa ccaaaccagg agaaaaagaa
     4861 acatccgaca ataaccacaa gtgatacaag ggtcagacga gtgtccgcaa aaatcatttc
     4921 cctgtttgcc cgggtaatag aaacaaatag agccgcagga agagcgtagt ttaataccag
     4981 tttattaaat gcccgcgctt gatcttctga aaaagtttct cgtctgccgc taaaataacc
     5041 caacagcatg attacgataa taggcaataa atcgccaata aaaaatgtta gcataatagt
     5101 atttccgata agggtcccct caccttcgca taatgagttg gcaagaggag acgatgtcag
     5161 tatttaatta ccagcgactt gttttgggtt aagtttggta atatggccac tttcagttcc
     5221 tgctgcaggg tcgatgacca cattaataat ggtcggtttg cgcgactgaa taccggtggt
     5281 taaagcatga cgaagttcat ctgtcgtggt gacgttatag ccaacgccac gaaacgcatc
     5341 cattaatttg tcataccttg catggtgcaa cagatctgtt ggtgatggtg caccagcgcc
     5401 actgagatca acaccgtctc ctctgtagat gccgccatta ttaaaaataa cgatcgtcac
     5461 cggcaggtta tatcgacaaa tcgtttcaat ctccatccca ctgaaaccaa aagcactatc
     5521 accttcaatg gcgacaaccg gagaaccaga ggtcacgcta gcaccgatgg catagcccat
     5581 accgatgccc atgacacccc aggtaccaca atccagacga cgacgtggtt tatacatatc
     5641 aataatattt cgtgcattat ccagggtatt tgcaccttca ttaactaaat aaatatcctg
     5701 gttctcacgc aatacatcgc gcacagcact taatgcatta aagtaattta atggttgggt
     5761 atctgtactt aatttttcat gcattttttg tgcattttgc tgcttgtgga tatttaaaat
     5821 atcgcgccat accagtggag tcgtaaatgt gttttgtttc agttctgcca gcataccttg
     5881 catactggat gcaatatcgc caacgactgg cacagcaatg gggcggttgc tgtcaatttc
     5941 ctgcggttca atatccagtt gaataaactg tgtatctgcc gcccatcctt ttttaccgtg
     6001 tgccagtaac caattcagtc gtgcaccaac aagcatgaca acgtcagcat ttgccagggc
     6061 aaacgaacgc gcagctgccg cagaaagtgg atgcgtatct tcgaggatcc ctttcgccat
     6121 agacattggc aggaatggaa tctgggcact ttcaataaat tcacgaagct gttcgtcagc
     6181 ttgtgaatac gccgcgcctt tgccaaggat aattaatggc cgttcagctt ttgctaaaag
     6241 cgaaattgcg ctagtgactg acttcgggca tggcaataat gctggcgacg gattttcaac
     6301 ttttacaatc gtggttaacg cttcgtcttt ttccatcgtc gcggccagga catttgctgg
     6361 caaatcaaga taaactccgc cagggcgacc cgatactgag acccggatag cgcgtgccaa
     6421 tgcaatgcca agatcctgcg gctgattaac gcgaaatgct gctttggcat acggttttgc
     6481 cgcattcatt tggtccagct cttcataatc accttgctgt aggtcgacga tcgcgcggtc
     6541 gctggagccg ctaatcatga tcatcggaaa accatttacc gttgcgttgg ccaatgcggt
     6601 caaaccattg aggaatcctg gcgcagacac tgtcaggcag atccctggtt tttgggtaag
     6661 aaaaccgctt gccgcagcgg catagcctgc cgactgctca tgacgaaaac caatataacg
     6721 aatgccttcc gcctgggcat ggcgcgccat atccgtcaca ggaataccta caacaccata
     6781 aatagtgtca atattattct gttttaatgc ttcaacgatg atatgcatac catctgtcat
     6841 ttgaagttga tctgacatga ttaataacct cgaaattttt ataaattggg agccagaagc
     6901 ccccgttaat atcagatggc gtggttttgc ttcattgcag caatttcatc gtcgctataa
     6961 cccagctcct gcaatacagc agcggtatgt tcacctaata gcggcgcagc tttaatatcc
     7021 ggcgtaaagt cagagaattt cattggacaa ccaactgtca gatattttcc acgcaacggt
     7081 tgttccactt cgacaacact gccactttgg cgcaaagagg gatcaattga aatttctttc
     7141 atacttaaaa ccggtgcaca aggaatatca aattgagtca aataggccac cgcttcatgt
     7201 ttatcaatag tgacagtgta tttttcaatt tcagcaaaaa tatcgaaaat atgtggctgt
     7261 cgggcatggg ctgtactgta tgccggatcg gtaatccaat ctggtttgcc gatggcttta
     7321 caggtgtttt cccagttttg ctcctgaata gtgaaataaa tataggcgtt aggatcggtt
     7381 tcccaacctt tacatttcag gatccagcca ggctgaccgc caccacccgc attacctccg
     7441 cggggaactg catcaccaaa tgtgccattc ggatactgcg ggtattcttc cagataaccc
     7501 aatttatcga gacgctgctg gtcgcgtaat ttcacgcggc aaaggttcaa tacggcatcc
     7561 tgcattgaca tggtgactcg ttgcccacgc cccgtttttt cgcgatgcag caaagcagca
     7621 agtaaaccga tcagtaaatg cattccggtg ttgctgtcac ccaacgctgc agcgcttacc
     7681 agcggcggac cgtcccaaaa acccgtagtg gatgccgcgc cacccgctgc ctgagcaacg
     7741 ttttcatagg cttttacatt cacataaggc gaacactcat caaacccttt gatcgaacca
     7801 aaaatcagac gtggattgat ttcttgaata tgctcccagg tgaaacccat gtgatcaatg
     7861 gcccctggat gaaagttctc gactaagata tcagcttcgc ggatcagctt ttccattacc
     7921 tctttgcctt ccgctgtttt ggtatttaac tcaatagaac gtttgttact gttaagcatg
     7981 gtgaagtaaa gcgcatcgat atcaggaata tctcgcagct ggtgacgcgt tacgtcacca
     8041 acgccgggac gttcaatttt aataacgtca gcgccaaacc aggccagcat ttgagtacaa
     8101 gatgggccag attgcacacc ggtgaaatcg agaactttaa ttccttgaag tggagttgac
     8161 atagcaatac ctattagtta attaaatatg tgttaattta gcgtttattt aaggcataac
     8221 gcctctataa ataatccgca aaatatcgtc gtagcggcac tcgatatttt tacattcctg
     8281 tatcaagtgc tgaaaatcat ttcatcgcta cttccaattc ctttctgcat ggtaagtagt
     8341 gaggacgaaa agaagaatat gataaatatt ggttcgtggt aatcagagag agtcgcgaca
     8401 tttgtgatgt tattcttaca actgtaagaa gcgccggtga tttacaagta agggtatgat
     8461 tacgcttttt aaagcgtacc ataaaatata acatattaaa tgatttgtga tgacacggga
     8521 ttattgcggt ttactttgag gaatataaaa tgcgagttta tagaacagcg agcgccaaat
     8581 aattttatga ccacaacctg tcgtattgtg gtcataaaaa attgtcggct ggagggagtt
     8641 aaatccttta cctcaatata ctgcctgtca gcattagttc acaaacagtg cttcgctatc
     8701 gacgatgatt ccatcttccg cacctttaag cgccaggttt gcctcgtagt attgcttttt
     8761 atcaagcagt ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg gcatcagata
     8821 ttggttttcc attacaccaa caccggccag gcgaagctct tccattgcgc cctttttatc
     8881 ccctttcgcc attttttcat tggcaatttt tatcgcggct tctttttcgg gtgtcgctac
     8941 ataactctca gaaattccga cagatgcatt aatgacaata tattggtcat cattgacatt
     9001 ggtcttttta cctggcttag cgaactttgc ccattcggta ctgtcatctg acaacaaagc
     9061 tgaagcttca ttggtcagtt cttttgcctt ttccggatcg ccatgaaaca gagccaggcg
     9121 cgctacctgc acgtcacgca tcgcatataa accttgttca gagatacgct ctgctgctgc
     9181 cgttttctgg gtttgctgtt gtgccgctac cggtgcatta tcagcagccc atacagttga
     9241 agatgccaga attgctgtga ccatcgtagc cataattaaa cgtttcatac ttacctcatt
     9301 ctttaacagt tttagaaaac gccgccatca tattgggtga gcatgaaatg agaaatcaga
     9361 gagcaacggt cattttgaaa gggttatctt acagttg
//
LOCUS       DAZKFU020000184         9368 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZKFU020000184 DAZKFU020000000
VERSION     DAZKFU020000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.186"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            169..309
                     /gene="ymiA"
                     /locus_tag="ACHOQQ_RS17905"
                     /old_locus_tag="pgaptmp_003581"
     CDS             169..309
                     /gene="ymiA"
                     /locus_tag="ACHOQQ_RS17905"
                     /old_locus_tag="pgaptmp_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGU4471790.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            312..479
                     /gene="yciX"
                     /locus_tag="ACHOQQ_RS17910"
                     /old_locus_tag="pgaptmp_003582"
     CDS             312..479
                     /gene="yciX"
                     /locus_tag="ACHOQQ_RS17910"
                     /old_locus_tag="pgaptmp_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGU4471791.1"
                     /translation="MVGQEQLESSPLCQDSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            593..688
                     /gene="ymiC"
                     /locus_tag="ACHOQQ_RS17915"
                     /old_locus_tag="pgaptmp_003583"
     CDS             593..688
                     /gene="ymiC"
                     /locus_tag="ACHOQQ_RS17915"
                     /old_locus_tag="pgaptmp_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGU4471792.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            852..3527
                     /gene="acnA"
                     /locus_tag="ACHOQQ_RS17920"
                     /old_locus_tag="pgaptmp_003584"
     CDS             852..3527
                     /gene="acnA"
                     /locus_tag="ACHOQQ_RS17920"
                     /old_locus_tag="pgaptmp_003584"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGU4471793.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3591..4181)
                     /gene="ribA"
                     /locus_tag="ACHOQQ_RS17925"
                     /old_locus_tag="pgaptmp_003585"
     CDS             complement(3591..4181)
                     /gene="ribA"
                     /locus_tag="ACHOQQ_RS17925"
                     /old_locus_tag="pgaptmp_003585"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGU4471794.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4351..5115
                     /gene="pgpB"
                     /locus_tag="ACHOQQ_RS17930"
                     /old_locus_tag="pgaptmp_003586"
     CDS             4351..5115
                     /gene="pgpB"
                     /locus_tag="ACHOQQ_RS17930"
                     /old_locus_tag="pgaptmp_003586"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGU4471795.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5264..5572
                     /gene="lapA"
                     /locus_tag="ACHOQQ_RS17935"
                     /old_locus_tag="pgaptmp_003587"
     CDS             5264..5572
                     /gene="lapA"
                     /locus_tag="ACHOQQ_RS17935"
                     /old_locus_tag="pgaptmp_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGU4471796.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5579..6748
                     /gene="lapB"
                     /locus_tag="ACHOQQ_RS17940"
                     /old_locus_tag="pgaptmp_003588"
     CDS             5579..6748
                     /gene="lapB"
                     /locus_tag="ACHOQQ_RS17940"
                     /old_locus_tag="pgaptmp_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGU4471797.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6941..7678
                     /gene="pyrF"
                     /locus_tag="ACHOQQ_RS17945"
                     /old_locus_tag="pgaptmp_003589"
     CDS             6941..7678
                     /gene="pyrF"
                     /locus_tag="ACHOQQ_RS17945"
                     /old_locus_tag="pgaptmp_003589"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGU4471798.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPYGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7678..8004
                     /gene="yciH"
                     /locus_tag="ACHOQQ_RS17950"
                     /old_locus_tag="pgaptmp_003590"
     CDS             7678..8004
                     /gene="yciH"
                     /locus_tag="ACHOQQ_RS17950"
                     /old_locus_tag="pgaptmp_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGU4471799.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAPPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDIDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8130..8348)
                     /gene="osmB"
                     /locus_tag="ACHOQQ_RS17955"
                     /old_locus_tag="pgaptmp_003591"
     CDS             complement(8130..8348)
                     /gene="osmB"
                     /locus_tag="ACHOQQ_RS17955"
                     /old_locus_tag="pgaptmp_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGU4471800.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8617..9366)
                     /gene="yciT"
                     /locus_tag="ACHOQQ_RS17960"
                     /old_locus_tag="pgaptmp_003592"
     CDS             complement(8617..9366)
                     /gene="yciT"
                     /locus_tag="ACHOQQ_RS17960"
                     /old_locus_tag="pgaptmp_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGU4471801.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         2323 a         2196 c         2454 g         2395 t
ORIGIN      
        1 caaattccag ttttcaaata tctatttcag gtcaaaagat tgaattattc actggagacg
       61 attcgtaaat tcgctggatt tttgactaaa gtttctttag gatttgtctc atcgtatgat
      121 taattacacc aatcgttcaa cgtcttgctg ataagtgatg gcgattatat gaggttagca
      181 atgccttctg gaaatcagga accccgccgc gatcctgaat taaaacgtaa agcctggctg
      241 gcggtttttc ttggttctgc acttttctgg gtggttgtcg cactgctgat ttggaaagtg
      301 tggggataac tatggttggt caggagcaac tggagtcgtc accattatgc caggatagtg
      361 acaatgaacc agaaacgaag cgggaatgtt ccgtcgttat tccagacgac tggcaactaa
      421 catcgcagca gcaagccttt atagaactgt ttgctgaaga tgatcagccg aaacaataat
      481 tatcatcatt cttattaccc atttttaatg aattaaaggg cttttaatac accgcagcaa
      541 taacaggttg agttacctca acacaaagta ataaccgtta agggtgtagc ctatgatcaa
      601 cacaaatatg aaatattggt cctggatggg tgcattttct ctgtcgatgc tcttctgggc
      661 cgaactcctc tggatcatta ctcactgatc cttgaccccg ctgcggcggg gttgtcattt
      721 gctttgccac aaggtttctc ctcttttatc aatttgggtt gttatcaaat cgttacgcga
      781 tgtttgtgtt atctttaata ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt
      841 aaggaggagc tatgtcgtca accctacgag aagccagtaa ggacacgttg caggccaaag
      901 ataaaactta ccactactac agcctgccgc ttgctgccaa atcactgggc gatatcaccc
      961 gtctacccaa gtcactcaaa gttttgctcg aaaacctgct gcgctggcag gatggtaact
     1021 cggttaccga agaggatatc cacgcgctgg cgggatggct gaaaaatgcc catgctgacc
     1081 gtgaaattgc ctaccgcccg gcaagggtgc tgatgcagga ctttaccggc gtacctgccg
     1141 ttgttgatct ggcggcaatg cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg
     1201 ttaatccgct ctcaccggtc gacctggtca ttgaccactc ggtgactgtc gatcgttttg
     1261 gtgatgatga ggcatttgaa gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt
     1321 atgtgttcct gaaatgggga aagcaggcgt tcagtcggtt tagcgtcgtg ccgccaggca
     1381 caggcatttg ccatcaggtt aacctcgaat atctcggcaa agcagtgtgg agtgaattgc
     1441 aggacggtga atggattgct tatccggata cactcgttgg tactgactcg cacaccacca
     1501 tgatcaacgg ccttggcgtg ctggggtggg gcgttggtgg gatcgaagca gaagccgcaa
     1561 tgttaggcca gccggtttcc atgcttatcc cggacgtagt gggcttcaaa cttaccggaa
     1621 aattacgtga aggtattacc gccacagacc tggttctcac tgttacccaa atgctgcgca
     1681 aacatggcgt ggtcgggaaa ttcgtcgaat tttatggtga tggtctggat tcactaccgt
     1741 tggcggatcg cgccaccatt gccaatatgt cgccagaata tggtgccacc tgtggcttct
     1801 tcccaatcga tgctgtcacc ctcgattaca tgcgtttaag cgggcgcagt gaagatcagg
     1861 tcgagttggt cgaaaaatac gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac
     1921 cgatttttac cagtacgtta gaactggata tgaatgacgt tgaagcgagc ctggcagggc
     1981 caaaacgccc acaggatcgc gttgcactgc ccgatgtacc aaaagcattt gccgccagta
     2041 acgaactgga agtgaatgcc acgcataaag atcgccagcc ggtcgattat gttatgaacg
     2101 gacatcagta tcagttacct gatggcgctg tggtcattgc tgcgataacc tcgtgcacca
     2161 acacctctaa cccaagtgtg ctgatggctg caggcttgct ggcgaaaaaa gccgtaactc
     2221 tgggcctcaa gcggcaaccg tgggtcaaag cgtcgctggc accaggctcg aaagttgttt
     2281 ctgactatct ggcaaaagcg aaactgacac cgtatctcga cgaactgggg tttaaccttg
     2341 tgggatacgg ttgtaccacc tgtattggta actctgggcc gctgcccgat cctatcgaaa
     2401 cggcaatcaa aaaaggcgat ttaaccgtcg gtgcggtgct gtccggcaac cgtaactttg
     2461 aaggccgtat ccatccgctg gttaaaacta actggctggc ctcgccgccg ctggtggttg
     2521 cctatgcgct ggcgggaaat atgaatatca acctggcttc tgagcctatc ggccatgatc
     2581 gcaaaggcga tccggtttat ctgaaagata tctggccatc ggcacaagaa attgcccgtg
     2641 cggtagaaca agtctccaca gaaatgttcc gcaaagagta cgcagaagtt tttgaaggca
     2701 ctgcagagtg gaaagaaatt aacgtcgcac gatccgatac ctacggatgg caggaggatt
     2761 caacctatat tcgtttatcg cctttctttg atgaaatgca ggcaacacca gcacctgtgg
     2821 aagatattca cggtgcgcgg atcctcgcaa tgctggggga ttcagtcacc actgaccata
     2881 tctctccggc gggcagtatt aagcccgaca gcccggcggg tcgatatcta caaggtcggg
     2941 gtgttgagcg aaaagacttt aactcctacg gttcgcggcg tggtaaccat gaagtgatga
     3001 tgcgcggcac cttcgccaat attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg
     3061 ggatgacgcg gcatttacct gacagcgacg tagtctctat ttatgatgct gcgatgcgct
     3121 ataagcagga gcaaacgccg ctggcggtaa ttgccgggaa agagtatgga tcaggctcaa
     3181 gccgtgactg ggcggcaaaa ggtccgcgtc tgcttggaat tcgtgtggtg attgccgaat
     3241 cgtttgaacg aattcaccgt tcgaatttaa ttggcatggg catcctgccg ctggaatttc
     3301 cgcaaggcgt aacgcgtaaa acgttagggc taaccgggga agagaagatt gatattggcg
     3361 atctgcaaaa tctacaaccc ggcgcgacgg ttccggtgac gcttacgcgc gcggatggta
     3421 gccaggaagt cgtaccctgc cgttgtcgta tcgacaccgc gacggagttg acctactacc
     3481 agaacgacgg cattttgcat tatgtcattc gtaatatgtt gaagtaacaa gtatttgctt
     3541 gccggttatt ttgcttccgg caagcaaatt aattacacaa tgcaagaggg ttatttgttc
     3601 agcaaatggc ccattttctc ggctttggta tcgagataat gttcgttatt ggggttacga
     3661 cctacaatca atggtacgcg ttcaacaata ttaatccccg cttcggtcag aatttcgact
     3721 tttttcgggt tattggttaa caagcggact tcattgacgc caaggagttt gaacatatca
     3781 gcgcaaagag tgaagtcgcg ctcatcagcg gcgaagccta actggtggtt agcctctaca
     3841 gtatcgtaac cttgatcctg cagtgcgtaa gcgcggattt tattcagcag accaatgtta
     3901 cgaccttcct gacggtgata cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat
     3961 gccgcttcga gctggaagcc acaatcgcag cgcaagctga acagggcgtc accggtcaga
     4021 cattcggaat ggacgcgcgc aagtaccggg gtatgcccgg aaatatcgcc atagactagc
     4081 gcgacatgat cgtgtccggt tgccagttct tcaaatccca ccatcaggaa atcgccccat
     4141 ggggttggca gtttggcttc tgccacacgt ttaagctgca tgaaattctc cagataatgc
     4201 tggttctgta ttggcttatt ttgccataac gagaaggggt tcacctaatc acgacgcgtc
     4261 gatcgttcac ggaatggcac aaatctgtca atttttcctg gaactggcat tttcagttat
     4321 gattgtggga cttatcaaaa aggagaggcc atgcgttcga ttgccagacg taccgcagtg
     4381 ggagctgcac tattgcttgt tatgccagta gccgtatgga tttctggctg gcgttggcaa
     4441 cctggagaac aaagttggct actaaaagcg gctttttggg ttactgaaac tgtcacccag
     4501 ccctggggcg tcattacaca tttgatttta ttcggctggt ttctctggtg tctgcgtttt
     4561 cgcattaagg ctgcctttgt attatttgcc attctggcgg ccgcaatcct tgttggacaa
     4621 ggcgttaaat cctggatcaa agacaaagtc caggaaccac gaccttttgt tatctggttg
     4681 gaaaaaacac atcatattcc ggttgatgag ttctacactt taaagcgagc agaacgcgga
     4741 aatctagtga aagaacagtt ggctgaagag aaaaatatcc cacaatattt gcgttcacac
     4801 tggcagaaag agacggggtt tgcctttcct tccggtcaca cgatgtttgc tgccagttgg
     4861 gcactactgg ccgttggttt gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg
     4921 ttggtctggg caactggagt catgggaagc cgcctgctgc tcgggatgca ttggccacgc
     4981 gatctggtag tagctacgtt gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg
     5041 caacgaattt gtgggccatt aacaccacct gcggaagaaa atcgcgaaat agcgcaacga
     5101 gaacaagaaa gttaacgctg gttgattttc cgaatttagc ccttaaatca tcaacaatgc
     5161 gcgtggatgc catttcgcag acggcgcgaa aatggtactt taaagggcta ttgcggtaag
     5221 ttgaccataa tttattcgct ctaaccacat aacgggaagt aatgtgaaat atttactcat
     5281 tttcttactg gtgttagcga tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca
     5341 acaggtgacg tttaattatc tgttagcgca aggggagtac cgtatttcca cattgctggc
     5401 ggtattgttt gctgcggggt ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg
     5461 agttcgtgtt tccctggcgc gcgctgaacg taaaataaag cgactggaaa accagctttc
     5521 acccgcgact gacgtggctg tagtgccgca ctcgtcagcg gcgaaggaat aactttctat
     5581 gctggagttg ttgtttctgc ttttgcctgt agccgctgcc tatggctggt atatgggccg
     5641 cagaagtgcg caacaaaaca agcaagatga agccaaccgc ttgtcgcgtg attacgtagc
     5701 gggggttaac ttcctgctta gtaatcaaca ggataaagcg gtagacctgt ttctcgatat
     5761 gcttaaagag gatacaggca ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc
     5821 gcgtggcgaa gttgatcgcg ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct
     5881 gacctatgaa cagcgtctgt tggcgattca acaactgggg cgtgattaca tggccgccgg
     5941 gttatatgac cgcgcggaag acatgttcaa tcagctgacc gatgaaactg acttccgcat
     6001 tggcgcgctg caacagttgc tacaaatcta ccaagctacc agtgagtggc agaaagcaat
     6061 tgatgttgcc gaacgcctgg tgaagctggg taaagataaa cagcgcgtcg aaattgccca
     6121 tttctactgt gagttagccc tgcagcatat ggccagcgac gatctcgatc gtgccatgac
     6181 cttgctaaaa aaaggggcgg cggcagataa aaacagcgcc cgcgtatcca tcatgatggg
     6241 acgcgtgttt atggcgaaag gagaatacgc caaagccgtc gaaagtctgc aacgtgtcat
     6301 atcccaggac agagaactgg ttagcgaaac gctggaaatg ttgcaaacct gctatcagca
     6361 gttgggtaaa actgccgaat gggcagaatt cctgcagcgc gcggtggaag agaacaccgg
     6421 tgccgatgct gaattgatgc ttgcggatat catcgaagcg cgcgacggta gtgaggctgc
     6481 acaggtctat attacgcgcc agcttcagcg tcatccgacc atgcgtgtgt tccataagtt
     6541 aatggattac cacttaaatg aagcggaaga agggcgtgcc aaagagagcc tgatggtgct
     6601 gcgtgacatg gttggcgaaa aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg
     6661 ttttaccgca tacaccctct actggcattg tccgtcttgt cgggcctggt caacaattaa
     6721 accgattcgc ggtcttgatg gcctgtaatt tttaaaaaaa tccgacttta gttacaacat
     6781 acttgttatt gatgttccat tgtgctccgg caacgacggc gcagaaaaag cctgccaggg
     6841 gagaaatcgc aactgttaat tttttatttc cacgggtaga atgctcgccg tttacctgtt
     6901 tcgcgccact tccggtgccc atcatcaaga aggtctggtc atgacgttaa ctgcttcatc
     6961 ttcttcccgc gctgttacga attctcctgt ggttgttgcc cttgattatc ataatcgtga
     7021 tgccgcgctg gcctttgtcg acaagatcga cccacgcgat tgtcgtctga aggtcggcaa
     7081 agagatgttt acattgtttg ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga
     7141 tatctttctt gacctgaaat tccacgatat ccccaacact gcagcgcacg ctgtcgctgc
     7201 tgcagctgac ttaggcgtgt ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat
     7261 gaccgcagcg cgtgaggcac tggttccgta tggcaaagat gcaccgcttt tgattgctgt
     7321 gacagtgttg accagcatgg aagccagcga cctggccgat cttggcgtga cactgtcacc
     7381 tgcagattat gcagaacgtc tggcggcact gacgcaaaaa tgtggccttg atggtgtggt
     7441 gtgttctgct caggaagctg tgcgctttaa acaggtattc ggtcaggagt tcaaactggt
     7501 tacaccgggc attcgtccgc aggggagtga agctggtgac cagcgccgca ttatgacgcc
     7561 agaacaggcg ttggcggctg gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc
     7621 ggtagatcca gcgcagacgc tgaaagcgat caacgcctct ttacagcgga gtgcatgatg
     7681 agtgattcca acagccgtct ggtctactca acagaaaccg gacgtattga tgagccaaaa
     7741 gcgcccccag tacgccctaa aggcgacggt gtggtgcgta ttcagcgtca gaccagtgga
     7801 cgtaaaggta agggcgtttg cctgattacc ggtatcgata tcgatgatgc cgaactgaca
     7861 aaacttgcag cggaactgaa gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt
     7921 attgaaatcc agggcgataa gcgtgattta ttaaaatcac tacttgaagc gaaagggatg
     7981 aaggtaaaac tcgcaggcgg ttaacataaa aagccacgga tatatccgtg gctttcgaat
     8041 attttactgt gtgtattatt cagttatttc gtactgaaca gaatcaagcg tattataatt
     8101 gcgagtcgat aatcgacatt tatttacgat tatttaccga cctggtgacc aataacacca
     8161 ccgacagctg cgccacctaa tgtacccaac gtactgccat cggtcagtac tgcaccgcct
     8221 aatgcccctg cacccgcgcc gattgcggtg ttgcggtccc gtttagacca gttagaacag
     8281 gcactcagag acattgccag ggtaattgcc agaacagccg cggtcatttt tttgctcgtt
     8341 acaaacataa tactctctcc tgaatttatg attcacggaa gtaagctctc tataactata
     8401 atagctaaga ataagcctgg tgaaattatt ccgtgaaatc cgctcgcgcg cagggttata
     8461 tcacgcaggt gataatgact tcctgttata tcgctgataa taattttata tcttgagagt
     8521 gttaataaca ggtaaatagt cttaattatc aaccaggaat catcttagag cggatgattt
     8581 gccaaactgc aaatcatccg tagagaaggg aaatggttaa atgtcaacga cgtgaacggt
     8641 gagtttactg tgctcaagat gcatcagatc gctggcgcgt attttcgaat cggtaatcac
     8701 gcgattgaat cgctcaacgg gaccgatgga gtatgaatgt acagcaccaa atttcgagct
     8761 gtcagtcagg actatcgctt cgcactcttt ttccagcacg gcattcacca catcggtacg
     8821 catcatatcg cgaccggtga atccagtttc aggttgccag ccatcaatac caataaatgc
     8881 tttgctgaaa tgtacctgtt ggatgcactg gcgtgtcaac gggccaacca tactttcgct
     8941 ttttttctgg tacacgccac cgagcaaaat aacttcacaa ggcgcgtctt tcagcaaatg
     9001 cgcgatgtag ctgctgaccg tgatgatagt gacatttttc ttctgctccc ccagagtccg
     9061 ggcgagcagg gcattgctgc tgccattttc gataaagatg gtttcgcccg gttgaaccag
     9121 cgacgcggca aactcggcga gttcacgctt cagcgtatag ttgctcatca tacgggtttc
     9181 gacgtcatca ctatcaagcg aaactgcaaa gccatgtgca cggcggaggt aactcagttt
     9241 ttcgagggtg ttgagatcct ggcgaatggt aacttcagaa actccagtgg cttttgccag
     9301 atcggttacg ctaacctgac cctggtcaat gaccatctgt agaatagttt gttgtcggga
     9361 attcatcg
//
LOCUS       DAZKFU020000185         9367 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZKFU020000185 DAZKFU020000000
VERSION     DAZKFU020000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.187"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(2..298)
                     /locus_tag="ACHOQQ_RS17965"
                     /old_locus_tag="pgaptmp_003593"
     CDS             complement(2..298)
                     /locus_tag="ACHOQQ_RS17965"
                     /old_locus_tag="pgaptmp_003593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471802.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNHLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            582..2213
                     /gene="oppA"
                     /locus_tag="ACHOQQ_RS17970"
                     /old_locus_tag="pgaptmp_003594"
     CDS             582..2213
                     /gene="oppA"
                     /locus_tag="ACHOQQ_RS17970"
                     /old_locus_tag="pgaptmp_003594"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGU4471803.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2299..3219
                     /gene="oppB"
                     /locus_tag="ACHOQQ_RS17975"
                     /old_locus_tag="pgaptmp_003595"
     CDS             2299..3219
                     /gene="oppB"
                     /locus_tag="ACHOQQ_RS17975"
                     /old_locus_tag="pgaptmp_003595"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGU4471804.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3234..4142
                     /gene="oppC"
                     /locus_tag="ACHOQQ_RS17980"
                     /old_locus_tag="pgaptmp_003596"
     CDS             3234..4142
                     /gene="oppC"
                     /locus_tag="ACHOQQ_RS17980"
                     /old_locus_tag="pgaptmp_003596"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGU4471805.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4154..5167
                     /gene="oppD"
                     /locus_tag="ACHOQQ_RS17985"
                     /old_locus_tag="pgaptmp_003597"
     CDS             4154..5167
                     /gene="oppD"
                     /locus_tag="ACHOQQ_RS17985"
                     /old_locus_tag="pgaptmp_003597"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGU4471806.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5164..6168
                     /gene="oppF"
                     /locus_tag="ACHOQQ_RS17990"
                     /old_locus_tag="pgaptmp_003598"
     CDS             5164..6168
                     /gene="oppF"
                     /locus_tag="ACHOQQ_RS17990"
                     /old_locus_tag="pgaptmp_003598"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGU4471807.1"
                     /translation="MNAVTEGRKVLLEIDDLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6221..6550)
                     /locus_tag="ACHOQQ_RS17995"
                     /old_locus_tag="pgaptmp_003599"
     CDS             complement(6221..6550)
                     /locus_tag="ACHOQQ_RS17995"
                     /old_locus_tag="pgaptmp_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGU4471808.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6585..8045)
                     /gene="cls"
                     /locus_tag="ACHOQQ_RS18000"
                     /old_locus_tag="pgaptmp_003600"
     CDS             complement(6585..8045)
                     /gene="cls"
                     /locus_tag="ACHOQQ_RS18000"
                     /old_locus_tag="pgaptmp_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGU4471809.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8188..8361
                     /locus_tag="ACHOQQ_RS18005"
                     /old_locus_tag="pgaptmp_003601"
     CDS             8188..8361
                     /locus_tag="ACHOQQ_RS18005"
                     /old_locus_tag="pgaptmp_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGU4471810.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8415..>9367)
                     /gene="kch"
                     /locus_tag="ACHOQQ_RS18010"
                     /old_locus_tag="pgaptmp_003602"
     CDS             complement(8415..>9367)
                     /gene="kch"
                     /locus_tag="ACHOQQ_RS18010"
                     /old_locus_tag="pgaptmp_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGU4471811.1"
                     /translation="YTLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFI
                     SFTTLLFYSTYGALYLSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTI
                     SVIISGITVFATSMTSIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQ
                     LNQRGQNVTVISNLPEDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALS
                     DNDADNAFVVLSAKDMSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVL
                     NGEEINNDMLVSMLLNSGHGIFSDNDEQETKADSKESVQK"
BASE COUNT         2424 a         2199 c         2345 g         2399 t
ORIGIN      
        1 atcatgttat tttcgccaca tcataatcct gggcttgctg aagaataatt gaaatgatat
       61 tagtaattcc actgcctttg gtagaggaaa gtgctaaata ataatcaatt gttaaatgat
      121 tgtgcatttc actactggaa ctgtaatcag aaaagataga catgcttaac caatctctat
      181 ttgattgaat tgaaagatgt ttgttaagac atggatgcaa gctatagatt ctgatacggt
      241 caataaaaga gaattgctta acaattttgc aaaatgtatt ggcgagtaag aagcgcattt
      301 ggtactttcc gggcaaccgc caggcgattc tttattggta atgagaataa ttaacaatta
      361 aagagcgtcg cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa
      421 ggtgatcgtc tgaatcacca gaataaataa agtcggtgat agcaatccgt aacgataaag
      481 taacctgaca gcagaaagtc tccgagcctg tgcagggtcc cattccggga ttacacatgc
      541 tggttaatac cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga
      601 gaagtttagt agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag
      661 ctgatgtacc cgcaggcgtc acactggcgg aaaaacaaac gctggtacgt aacaatggtt
      721 cagaagttca gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc
      781 gagacctgtt tgaaggctta ctggtcagcg atcttgacgg ccatccggca cctggcgtcg
      841 ctgaatcctg ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga
      901 aatggtctga tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg
      961 ttgatccgaa caccgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta
     1021 ttgatgaaat tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg
     1081 atcacacatt agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc
     1141 acccatcaac ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc
     1201 agcctggtaa tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac
     1261 gaatcgttct tgaacgcagc ccaacctact ggaacaacgc gaaaaccgtt attaaccagg
     1321 taacctattt gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa
     1381 tcgacatgac ttataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc
     1441 cggacgaagt tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga
     1501 aaccgccatt caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata
     1561 tcattgttaa taaagtgaaa gcgcagggcg acatgcccgc ctatggttac actccaccgt
     1621 atactgatgg cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta
     1681 acgaagaagc gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca
     1741 tcaacctgct gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat
     1801 tgtggaagaa aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc
     1861 tcgacacccg tcaccagggt acttttgatg tggcccgtgc aggctggtgt gctgactaca
     1921 acgaaccaac ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt
     1981 ataagagccc ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc
     2041 agcgcacagc tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc
     2101 ctgtttatta ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggt tataccggca
     2161 aagatccgct ggataatacc tacacccgga atatgtacat tgtgaagcac tgatggcaat
     2221 acgtggggca ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac
     2281 aggccagaag gtagggcaat gttaaaattt attttacgtc gctgtctgga agcgattccg
     2341 acgctattta ttcttattac tatttcgttc tttatgatgc gcctcgcgcc gggaagtcct
     2401 tttaccggcg aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat
     2461 cttaatgatc cgatcatgac acagtatttc agctacctga aacaactggc gcacggtgat
     2521 ttcggtccat cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt
     2581 cccgtttctg ccaaactggg agccgcagca tttttccttg cggtaatact gggtgttagt
     2641 gctggcgtta ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg
     2701 gcaatgaccg gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt
     2761 gcgatcattt tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg
     2821 atattgccga tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt
     2881 ggctctatga ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta
     2941 cctatgcggc ggatcatttt acgccacgca ttaaaacctg ctctgttacc cgtgctctcc
     3001 tatatgggcc ccgcatttgt cggcattatt accggttcta tggttatcga aaccatttat
     3061 ggtttgccgg ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta
     3121 gtgttaagcc tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat
     3181 gtgctatatg cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt
     3241 taagtaagaa aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc
     3301 gcagcttgtg gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc
     3361 tgatagtgct ggtgctgatc gcgttatttg taatcctggc accgatgctt tcgcagtttg
     3421 cctatgacga tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact
     3481 actttggtac tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta
     3541 tctcactcat ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttacg
     3601 gttcgctttc cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa
     3661 tcctcaactc cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa
     3721 acatcctgct gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg
     3781 tgcgtgggca aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg
     3841 gtgtatcgac gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg
     3901 tggtctacgc atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc
     3961 tggggttggg tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga
     4021 actcgatgga agtctctcca tggttactgt tgttcccagc gggattcctc gtggtgacgc
     4081 tgttttgttt caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt
     4141 aaggagtgca gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc
     4201 tgacgcactg ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt
     4261 cacggcggtc aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg
     4321 tgagtctggt tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa
     4381 cggacgcatt ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg
     4441 tgaactcaat aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc
     4501 gttgaatccc tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tacataagaa
     4561 catgagcaaa gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc
     4621 ggaagcacgt aaacgcatga aaatgtaccc gcacgaattt tctggtggca tgcgtcagcg
     4681 agttatgatt gcgatggcat tgctatgtcg acctaagctg ctgattgcag atgaacccac
     4741 tactgcgctg gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg
     4801 ggaatttaat accgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg
     4861 cgacaaagtg ctggtaatgt acgccgggcg cacgatggaa tatggcaacg cgcgcgatgt
     4921 cttttatcaa cccgttcatc cttattctat cggtttgctc aacgcggtgc ctcgtctcga
     4981 tgcggaaggt gaaacaatgt tgactattcc gggtaatccg ccaaacctgc tgcgattacc
     5041 gaaaggttgc ccgttccagc cacgttgtcc gcatgcgatg gaaatttgta gtagcgcacc
     5101 gccgctggaa gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact
     5161 gttatgaatg ctgtaaccga aggaagaaaa gtcctcctcg aaatcgacga tctgaaagtg
     5221 cactttgaaa tcaaagatgg caaacagtgg ttctggcaac caccgaaaac tctcaaagcc
     5281 gtagatggtg taactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg
     5341 ggatgcggta agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt
     5401 catgttgcct ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt
     5461 cgcagtgata ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc
     5521 atcggcgaga tcattgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa
     5581 gttcgcgagc gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac
     5641 cgctatccgc atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt
     5701 attcttgaac cgaagctgat tatctgcgat gagccggttt cggcgctgga tgtgtcaatt
     5761 caggcgcagg tggtcaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt
     5821 tttattgctc atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat
     5881 ctcggccatg cggtagaact ggggacctat gatgaggtct accacaatcc actacatcct
     5941 tacaccaggg cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa
     6001 accatccagt tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt
     6061 tttcgtaccc gttgcccgat tgccggtccg gagtgtgcca aaacacgtcc tgtgctggag
     6121 gggagtttca gacacgccgt ttcttgcctg aaagtcgatc cgctttaaac acaaaaaggg
     6181 ctgacagttg tcagcccttt ttcacgccga aagtgattat ttattcccgc cagatgatat
     6241 ggcaaagttt gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgttgattt
     6301 caccatcttc actgtccgcc agaccaatca ccacttcggc aaaaaagtct gggttcaaat
     6361 cgaaatcaac atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct
     6421 cttcaaactg aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt
     6481 cgagaaaaat atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat
     6541 ccatatccat agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg
     6601 aagaagtaaa acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg
     6661 gcatcgagca gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca
     6721 aaacctttat cgtcgattgc cagggtaatc tcgaaattca gccacagact acgcatatca
     6781 aggttaacgg tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt
     6841 aacccgcctt caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg
     6901 cgactggccc agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc
     6961 accccgcgct gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag
     7021 ggcgtggtca tgatcaaata ttcacgcgcc gaataagccg cagtcaataa tgcctggtga
     7081 atgagatctt ccggaaagcc ggggccagaa gcaattgtgt gaatggtatg accgctggcc
     7141 tgctcaaacg gcataatatt gacatcaggt ggtggcggta gaatacgttt ccctgtttca
     7201 atctcccagt cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc
     7261 gccatcagat caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc
     7321 atattcatgc tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg
     7381 cgcaggtcca tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact
     7441 tcaataccgg cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg
     7501 gcggagtcga gcatcaatcg gcaatgaatg ccgcgtcgcg cagccgccat taatgattca
     7561 gccacctggt ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg
     7621 cgcgcgagct ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagt
     7681 tgtagctgat tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat
     7741 ggcgcagcga cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta
     7801 agccattttg cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa
     7861 tggagctcgc caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc
     7921 aacagccagg ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg
     7981 cctgcaatga gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt
     8041 gtcatagatt agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat
     8101 ctggcaaata aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc
     8161 attctgttat tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg
     8221 cgcatgcagc gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga
     8281 aatatgcgta tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg
     8341 tttgcgatct acaatatcta aatgttctgg gcaccattgc ggtgcccaat aagtttactt
     8401 ttccgtcttt aactctattt ttgcaccgat tctttactgt cagctttcgt ttcttgttca
     8461 tcgttatcgc tgaaaatgcc atgaccggag ttcaacaaca ttgaaacgag catatcatta
     8521 ttaatctctt caccatttaa aactcgcgcc agaatttcgc tgccaaacag ttgcggtgag
     8581 agaatgatat ccggatgtac catcttaatc ttattcaggt ttttgctatc actgacggcg
     8641 agaactgttt tgacatcact gctcatatct tttgccgaga gtacaacaaa cgcgttatct
     8701 gcatcgttat cactcagcgc cagaatggcc cggcaacgat cgattcccgc tttctttaat
     8761 acagaactgt cattactgtc accggggata acgtctgcgt tatcgcctaa gcgttgctca
     8821 agttgcttga tatcatcttc aggcaagttg ctgataaccg ttacgttttg tccgcgttga
     8881 ttcagttgca ggatcgtatt gatggcgaga atcgaatgtc cgcaaacaat aaaatgatct
     8941 ttacgatgca ttgtatgatt gtttcctttt acaagtttgt tgaatccccc gcggataagc
     9001 ggaccaaaaa ttgaggtcat agatgtggca aaaacggtaa tgccggaaat aatgaccgaa
     9061 atagtgaaca atcgtgctga ttcagaaaca gggacaatat cgccatagcc gacggttgac
     9121 atggtttcta tcgaaaaata gaacgcggtc atcaaacttt ctattcgcgg attaaaacct
     9181 tcgcttaaat aaagcgcacc gtaggttgag taaaacagta atgtcgtgaa actaataaat
     9241 gcaaaaattg tcccggctgc ggcgctactg tgggagaagt ctttgcgcag tatcagcaaa
     9301 aacaccagcg taaaaatgca aaatccaata ctaaatttca gccagggata aaaatgcagg
     9361 gtgtaaa
//
LOCUS       DAZKFU020000186         9351 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZKFU020000186 DAZKFU020000000
VERSION     DAZKFU020000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.188"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            500..3247
                     /locus_tag="ACHOQQ_RS18015"
                     /old_locus_tag="pgaptmp_003603"
                     /pseudo
     CDS             500..3247
                     /locus_tag="ACHOQQ_RS18015"
                     /old_locus_tag="pgaptmp_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
     gene            6140..7000
                     /gene="pdxI"
                     /locus_tag="ACHOQQ_RS18020"
                     /old_locus_tag="pgaptmp_003604"
     CDS             6140..7000
                     /gene="pdxI"
                     /locus_tag="ACHOQQ_RS18020"
                     /old_locus_tag="pgaptmp_003604"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGU4471812.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDILDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            7064..>9351
                     /gene="ydbD"
                     /locus_tag="ACHOQQ_RS18025"
                     /old_locus_tag="pgaptmp_003605"
     CDS             7064..>9351
                     /gene="ydbD"
                     /locus_tag="ACHOQQ_RS18025"
                     /old_locus_tag="pgaptmp_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGU4471813.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALKIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIASMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWGFLESALISWDNLPAVVLKELQQNTSR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMR"
BASE COUNT         2508 a         2098 c         2526 g         2219 t
ORIGIN      
        1 ttgttacgca cggtctagat ctaaggaatt ttatatgcaa aggaaaaaat tattgtctgt
       61 ttgtgttgcc atggcgttaa gtagccagac atgggctgcg gatacttcaa tacccgactc
      121 aaccgaaaaa acacgtaagt ccagtaagat aacatgtccg gcaaatattc attccctgag
      181 caaagagcag ctagaaaatt tatccgcgga gtgcagagaa aataaagata gtgcagtttt
      241 accatgggca gctgcaggtc ttgctgcaat agctacggga attgctatat ataccttaag
      301 tgacgatgac aatcaccacc ataataactc cccagttccg gatgatggcg gtgatacgcc
      361 tgttccgcca gacgacggcg gtgatacgcc ggttccgcca gatgacggcg gtgatacgcc
      421 ggttccgcca gatgacggcg gtgatacgcc agttccgcca gatgacggcg gtgatacgcc
      481 agttccgcca gatgacggcg atgatacgcc ggttccgcca gatgacggcg gtgatacgcc
      541 agttccgcca gatgacggcg gtgatacgcc agttccgcca gatgacggcg gtgatacgcc
      601 ggttccacca gatgacggcg gtgatacgcc ggttccacca gatgatggcg gtgatacgcc
      661 agttccagac gacggcggtg atacgcctgt tccgccagac gatggtggcg ataccccggt
      721 taaacacgat cctgtcgtct ataaaaatga cgtaacctgg gatcaggacg cgaaaaccgt
      781 caaaattcgc gaaaccacct ttacctacag ccaaaatgcc gatgggagtt acaccttaac
      841 cgcgcctgat ggcctgacga cgatagtcaa gggctggaca gtccatgacg acagcaacac
      901 cgtcatcttt gatggcgtaa atacggcagg tggcatcacc tggagctacg acgatgatgg
      961 gctcatccat atcactaaag aggccggtgt cgttgttgat ggcacaaccg gaactgcgat
     1021 tgattttggt aatgcgatca tcaccgatca gggcggcaat acggcgctga atggcggtac
     1081 tgtcatgacg gttgagggtg acaacatcac cctcaacaac gacggtaaaa cgaccgccat
     1141 cggcgagggt tcggtcgttg gtatccttac tggtgacgat atcactatca ataacaacgg
     1201 cgagacggaa gttgacggcg gtaccgcagt aatcatcaat ggcgataatg ctacgctgaa
     1261 cactgcgggc gactcgacca tcaccaatgg tggtacgggt agccttatca atggcgataa
     1321 tgcgcgggtc gataatcaag gtaccatgag cgttgacggt gagaattcca ctggctcgaa
     1381 aattgtcggt gacggtgcca ccatcaaaca ggaaggcgac ttatacgtaa gcggtggtgc
     1441 gcacggtatt gacgttgacg gcaatgatac gttcgttagc aacaaaggta atattacggt
     1501 catcgaagat aactctattg gcatgctgct tgatggtgat ggcgtcagcg tgatcaacat
     1561 gggcgacctc aatgttggtc aggccgctgc gggtgaaaat gcaatcggca ttcagatcga
     1621 cggtgataat gcgacatttg ttaacgttgg tgatatttcc gcgaccaatg ccggaaccgg
     1681 tgtcagcgtt gctggggata aagctaatat ttcactcgcg ggtgggttag acgttgggga
     1741 tttctccacc ggtcttgatg tctctggcaa taataacaac atgacgctgg cgacttatga
     1801 gctgaatgtg acagggcaaa aagcgacggg ggttaatgtt tcgggtgatg gcaataggat
     1861 tgaaatcgca ggcagcattc tggttgataa agatcagaaa gccgataatg cgcaacctta
     1921 cttctttaat ccctcaaccg gcgtaaatat cagcggtaat aacaatgacg tcacgctgga
     1981 tggtcagtta accgttgttg ctgatagcga aaccacggtg cgttcctatg caagttacga
     2041 cggcgctcag gagcacattg caggtattgt tattgctggc gatgataata cctttacgct
     2101 taatggcggt gtccattttg tcggcgaaaa aaatgtcatg gatgatggct caaaaccatc
     2161 tgcttctcgt cgtggtatcg gagacacacc gcttattagc gttgatgggc attcatcggt
     2221 ctacctcaat ggtgagtcaa ccattagcgg cgagttcccg ttgggtttcg aaaatctcat
     2281 tcagttgagt catggcgcgg agctggagat tggcgctgac gcgacgtttg atatgagtga
     2341 tgtggattcc ttcacctatt attatcgcgt ggcgttatcc accattagta ttgattctgg
     2401 tgcgaaggcg accaataacg gtgaagttga actgaaaaat atcggttttg cggctgcctg
     2461 gaataaagat tcaacggtca tcaataacgg atccattggc ctggcgatgt acgattttgg
     2521 taccgacccg gcaccaaaag tcttcgatgt tgaatacggg gggattggcg ttaacaatgg
     2581 caccttgacc accaaaatga tgaatcagca cagcgtgctg aattatccgg cagagtggaa
     2641 cttgtctgac ggtacctctt tcaataacag agcattaggt ttgacaggta tgcttgccag
     2701 ctactcgtca tccattctga acggcgaaac gggcattatt gagatgtatg gtcgtggcag
     2761 cgtggggatg ttggctatcg ataaatcaac cgcggataac gaagggcaaa tcaccctgga
     2821 tacgctgtgg gtcgatgaaa atgatgaaac atcgctgcgt aataacatcg caaatagcac
     2881 ggcgaaagat ttcggtgtgg gaatggcttc gggaaccgat gcttactatg gagcattaac
     2941 caaagcgacg tctaccaacc acgagaatgg tgtgattacc gtctacaacg cgggtgcggg
     3001 gatggcggcg tatggtaata ccaacacggt cattaaccag ggaacgatca accttgagaa
     3061 aaatgaaaat tacaacgact cgcttggcgt aaataaactc gtcggcatgg ctgtttacga
     3121 gaaaggaacg gctatcaatg atcaaaccgg tgttattaat attaatgctg aaaatggtca
     3181 ggcattctat aacgatggca ctgggcttat tatcaactat ggcaccattt gtacctttgg
     3241 cgtttgtcag ggggccgatg agtataatcc gacggataac tatatcgccg atttgtatga
     3301 tgatggcgat gtattaagct ctgcgggtga gactgtatca ttatcatctg gtgcgattat
     3361 taatggggat gttaccagcg cgggtaccgt taagaatgga aaaatccaga ttcttgaaaa
     3421 tggcgcgctg gttaatgagt cgagcggaac catcaattcg gctatcgtcg ttaatgcagg
     3481 cggtacgtta accaatgaag gtgtattgaa tacggtgacg ctggaaggag gcactttcaa
     3541 taacaacgga acgcttaatg acgtcgtaaa aattgagaaa aacagcaacg cggtaattaa
     3601 taataccggt tcactgtcaa ccttacagct taacgatggc acggtgaata acagcggtat
     3661 cgcgtcggcg cgcgttaacg cccagggcga tgctgtattc aataaccttg caggcggcga
     3721 ggcgcgtaaa ggtgccattc tctataactc tgcggtggtg aataacgcgg ggacctggaa
     3781 aatgggttat caggatgaaa ataacaatgc cgggacgctg gatattgacg accaatcaac
     3841 gttcaacaac agcggcaagc tcatccttga taacagtaaa aacgccattc gcttccaggg
     3901 aagcaatgct aacgccacgt tatataacac cggtgaaatg acgctggatg ccgcacttgg
     3961 cgcgggcgct attcactatg acgacggcgc cagccagttt attaacaagg gcgtggtgga
     4021 tgcgaaagtc actgtggcgg taagtaccgc tggtgcgaca gaaagcgatg ccatcctgtg
     4081 gaaccaggat ggcggggtga ttaacttcga taaagacaac gccagcgcag ttaaattcac
     4141 ccacaacaac tatgttgccc tcaacgatgg cgtgatgaac atcagcggca acaatgccgt
     4201 ggcgatggaa ggtgataaaa acgcacaact ggttaacaac ggcgttatca atatcggcac
     4261 cgaaggtacc accgataccg ggttaattgg tatgcaactg gatgccaacg ctaccgcaga
     4321 cgcggtgatc gagaacaacg gcacgattaa tatctatgct aacgattcct ttgcattcag
     4381 tgtgttggga acacaaggcc atatcgttaa caacggtacc gtggtgattg ccgatggcgt
     4441 gaccggttcc gggttgatta agcagggcga cagcgtgaac gtggaagggg tgaacggcaa
     4501 caacggtaat aataccgaag tgcattacac cgactacacg ttgccggaag tgccgaacac
     4561 cagttctggt ggcgtaacaa cagggggaga tgcgactggc agcggtagca acaacctcaa
     4621 cggttatgtg gtcggtacca acgttgacgg cagcgccggt aagctcaagg tcaacaacgc
     4681 cagcatgaac ggtgtcggga tcaacaccgg tttcgccgcc ggtacggcag acaccacggt
     4741 cagtttcgac aacgtggtgg aaggcagcaa cctgaccgac gccgatgcca tcacctcaac
     4801 gtccgtggtg tggaccgcca aaggcagcac cgatgccagc ggcaacgttg acgtcaccat
     4861 gagcaaaaac gcctacaccg atgtggcgac cgatgcttcg gtgaacgatg tggcgaaggc
     4921 actggatgcg ggttacacca acaacgagct gtataccagc ctgaacgtcg gcactactgc
     4981 cgaactgaac agcgccctga agcaggtgag cggcagccag gcaaccacgg tattccgcga
     5041 ggcgcgcgtg ctgagcaacc gcttcagcat gctggcggac gccgcgccga agatgggcaa
     5101 cggtctggcg tttaacgtgg tggcgaaagg cgacccgcgt gctgaactcg gaaataacac
     5161 cgagtacgac atgctggcgt tgcgtaaaac cgttgacctg agcgaaagcc agagcctgag
     5221 tctggagtac ggtatcgccc gtctggatgg cgacggtgcg cagaaagcgg gcgacaacgg
     5281 cgtaaccggt ggttacagcc agttcttcgg cctgaagcac cagatgttct tcgacaacgg
     5341 gatgaactgg aacaacgccc tgcgctatga cgtgcataac ctcgacagca gccgctcggt
     5401 ggcttacggc gacgtcagca aaacggcaga cacggatgtg aaacagcagt acctggagtt
     5461 ccgcagcgaa ggggcaaaaa cctttgaacc gcgcgaaggg ctgaaaatca ccccgtacgc
     5521 cggagtgaaa ctgcgtcaca cgctggaagg cggctatcag gagcgcaatg ccggagactt
     5581 taacctgagc atgaacagcg gcagcgaaac ggcggtggac agcatcgtcg ggctgaaact
     5641 ggactacgca gggaaaggcg gctggagcgc gaatgcgacg ctggaaggcg ggccgaacct
     5701 gagctacagc aagagccagc gcacggcaag ccttgcaggg gcaggcagcc agcactttaa
     5761 cgtcgatgac ggtcagaagg gcggcggtat caacagcctg gcgagcgtcg gcgtgaagta
     5821 cagtagcaaa gaaagttcgc tgaatctgga tgcgtatcac tggaaagagg acggcatcag
     5881 cgacaaaggc gtgatgctga actttaagaa aacgttctaa tttttagcat gtgatacaca
     5941 aaccgcaacg ctgatacagg ttgcggtttt ttattgccgg atgcgacgct tgacttatct
     6001 tttgctgtct aatgttttgg tgtctttcag gacaatagtg ctactcatcc agaaatcttg
     6061 tttacgcctt cgcattattt atctcttttc gtttctatac tgatttttct taatccgttt
     6121 tattacaggg cagggtgcga tgagcagcaa tacatttact ctcggtacaa aatccgttaa
     6181 ccgtcttggt tatggcgcga tgcaactggc aggtcctggg gtttttggcc ccccaagaga
     6241 tcgccacgtc gctataaccg tgctgcgtga ggcgctggca ttgggcgtca atcacattga
     6301 taccagcgac ttttatggtc cgcacgtcac caatcagatt atccgcgaag cgctttatcc
     6361 ttactctgac gatctgacaa ttgtcactaa aattggtgcg cggcgtggag aggacgcatc
     6421 ctggttgccc gcattttctc cggcagagct gcaaaaagcg gtacacgata atctacgtaa
     6481 tctcggcctg gacatactgg atgtggttaa cctgcgcgtg atgatggggg atggtcatgg
     6541 cccggcggaa ggatcgattg aagccagcct gaccgtgctg gcagagatgc aacaacaagg
     6601 cctggtaaaa catattggcc tgagcaacgt cacaccgacg caggttgcag aggcgcgcaa
     6661 gattgccgaa atcgtctgtg tgcagaacga atacaacatt gctcatcgtg ctgatgacgc
     6721 aatgattgaa gctttggctc gtgacggcat tgcctacgtg ccgttctttc cgctcggggg
     6781 ctttacgccg ctgcaatctt ccacgctttc cgatgttgct gcgagcctgg gtgcaacacc
     6841 aatgcaggtg gcgctggcgt ggctgttaca acgttcaccg aatattttgc tgatcccagg
     6901 gacgtcttct gttgcgcatt tacgggagaa tatggctgct gaaaaattgc atctttctga
     6961 ggaagtgttg tctacgttgg atggtatttc gcgagaataa cgaatataca aaagggaaag
     7021 atgcatttcc cttttgtttc ttttttaatg gcatggagtg catatgttta agcccaggat
     7081 ttgcagctgg ataggtttac tccccctgtt tatgctgagt cttccagtac aggcggaact
     7141 tcgatgtgtt gcaaatgcag ttgatattga accatttttt tctgcggcta ccgctgaaga
     7201 taaacaacaa gttgaacaag ctatcaacag tagcgtgaat cttgtcccct tcggtttatc
     7261 tgcatcggac tggaaagtgc atcgtggcga tttagtggta gaaggtaata tagagagtaa
     7321 tcaaaaattg attgttcttg gcaatttgac agtcaaaggt aatatttcca ctttctctct
     7381 ttctaatcca tgggttattc tcggtaacgt gacggcaacg aatatagtta cggattcacc
     7441 actgttaatt accggttcga taaatgcaag tggactggta tttatcgact catattacga
     7501 taatccgtct acgattaagg ggagtattaa tgcgcgtggg atatttatca atgacataat
     7561 tgcacctgtt gttgcgtctt cgacaaatag tgaattcatg gtccgtgcga gtgataaaaa
     7621 tgacactgaa aatgtcaaaa aagcgttgaa gataataaat cctgatgcat attattgggg
     7681 tctaataaat gatgaagatg ctctgaaaga aatttttaag cgaagcaata ttcgcatggc
     7741 agggaatgtc tgtaatcaga tgaaaaaaga agcgctgttt cgccctaagc cttctcctga
     7801 gttagtgcag gaattgcaaa tgctggatga aggcaacgtt gctgcatttg aaggacgaga
     7861 tattgcgaca tttgatcttg ccataatgcg aactctccca aggcttaaag gaatttcagc
     7921 taacctacgc aaacaactta ttaatagcaa tgatggacaa acgattgcaa gtatggcgag
     7981 atatatgcct gacaatgaga ttctggagtt gaccgatcag caattgggtt accagcctgt
     8041 tgttctgggg ttgctcgacc gtgaaccgct ctctgtcgaa ataatgacgc gaatgagtca
     8101 tctacctgat ggtgtcggtc cgttgaatct tgcgctacgt gaaaatctcc ctctggatat
     8161 cgtcatgact ctggcgaaaa gagattggga tatgatcatt caggagcttt ataaagatgc
     8221 gtggttattg cccgaatcca ttattgatgg ctatatccgc agtgatgatt cctctattcg
     8281 tcaggtcggt gctggaggac aactcaccta caatcaggca atgcagttgg cgaacgattc
     8341 atcaaacgat gttgtcacaa gcttagcgtt aaagctggca gagatgaaac accatggtca
     8401 attgttgcgg atgacgccac aagagagtga caaaatagca gtatatttat accaaaaatt
     8461 cgagaatgac gatgatctaa taggtgcgtt atttttagcg ttgccagata acttacagtt
     8521 taattttgtt aaaaggatgg agaaaaaatc ccctgcctac ttttgctgtc gggatatgca
     8581 gataattcac tctgacgctg ctttacaacg gttattgaca cgtttcaacg atcctgaggg
     8641 gtggagtaat ctggcgaaaa atcagtatct gagcacgtcg atgaaacaaa aaatttggca
     8701 acgtgcttta tcgcatcgga aaaataatcc caaagcagat tcagatgcat atgaaaccag
     8761 cgctgatatg attttgtctg agcttatcag ctacggtgaa gttgatgacc agatgctatt
     8821 aaatgccacc tctctgatac gttcggacga ctggggcttt ttagagagtg cattaattag
     8881 ttgggataat ttacccgctg ttgttctcaa ggaattacag caaaacacat cacgcaatga
     8941 tatttgggcg aagttttttc tgaggcagga aaacagttcc cgtgcgcagg ttaacgaagc
     9001 gttacgtgtt tattatgcac tagaccccga tgcgttagca caactggatg tactggcaaa
     9061 acaaccggat cgtatatggt ggagtacact ggcgaaaagc aatctcacat ttttcaagtt
     9121 cggcgcactt aacaaccgcc acacaccgcc tgcagtactg gcggcagaaa ttgatcccga
     9181 gtggtggatt gtggcgatga ataatccccg ttttccagtt gatgtattaa aggcgaggct
     9241 gaaacgtgat cctttgctgg cgttagagct tgttaaccct gaactggatt tagttcgcca
     9301 gctggcgctt aacggtaaga cgcgcgcaat acgggaacaa gcgatgagaa a
//
LOCUS       DAZKFU020000187         9221 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZKFU020000187 DAZKFU020000000
VERSION     DAZKFU020000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.189"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2092
                     /locus_tag="ACHOQQ_RS18030"
                     /old_locus_tag="pgaptmp_003606"
     CDS             <1..2092
                     /locus_tag="ACHOQQ_RS18030"
                     /old_locus_tag="pgaptmp_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGU4471814.1"
                     /translation="FDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGG
                     TINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGT
                     MAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYV
                     KNKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQ
                     LNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVA
                     HGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYP
                     SSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNRE
                     GSKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQ
                     GFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYR
                     SNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDP
                     LQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQ
                     MAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREII
                     KAGSLINFNKNRQM"
     gene            complement(2233..3516)
                     /locus_tag="ACHOQQ_RS18035"
                     /old_locus_tag="pgaptmp_003607"
     CDS             complement(2233..3516)
                     /locus_tag="ACHOQQ_RS18035"
                     /old_locus_tag="pgaptmp_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGU4471815.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3668..4144)
                     /locus_tag="ACHOQQ_RS18040"
                     /old_locus_tag="pgaptmp_003608"
     CDS             complement(3668..4144)
                     /locus_tag="ACHOQQ_RS18040"
                     /old_locus_tag="pgaptmp_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGU4471816.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(4203..5492)
                     /gene="bioA"
                     /locus_tag="ACHOQQ_RS18045"
                     /old_locus_tag="pgaptmp_003609"
     CDS             complement(4203..5492)
                     /gene="bioA"
                     /locus_tag="ACHOQQ_RS18045"
                     /old_locus_tag="pgaptmp_003609"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGU4471817.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIIHPQQLQRLTAGVNRAVQDETFFCQ"
     gene            5579..6619
                     /gene="bioB"
                     /locus_tag="ACHOQQ_RS18050"
                     /old_locus_tag="pgaptmp_003610"
     CDS             5579..6619
                     /gene="bioB"
                     /locus_tag="ACHOQQ_RS18050"
                     /old_locus_tag="pgaptmp_003610"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGU4471818.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            6616..7770
                     /gene="bioF"
                     /locus_tag="ACHOQQ_RS18055"
                     /old_locus_tag="pgaptmp_003611"
     CDS             6616..7770
                     /gene="bioF"
                     /locus_tag="ACHOQQ_RS18055"
                     /old_locus_tag="pgaptmp_003611"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGU4471819.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVTQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFIHNDVAHLAR
                     LLASPCSGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTSAHEMQDIDRLLEVLHGNG"
     gene            7757..8512
                     /gene="bioC"
                     /locus_tag="ACHOQQ_RS18060"
                     /old_locus_tag="pgaptmp_003612"
     CDS             7757..8512
                     /gene="bioC"
                     /locus_tag="ACHOQQ_RS18060"
                     /old_locus_tag="pgaptmp_003612"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGU4471820.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLVWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYRHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            8505..9182
                     /gene="bioD"
                     /locus_tag="ACHOQQ_RS18065"
                     /old_locus_tag="pgaptmp_003613"
     CDS             8505..9182
                     /gene="bioD"
                     /locus_tag="ACHOQQ_RS18065"
                     /old_locus_tag="pgaptmp_003613"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4471821.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPRLAENPENAATGK
                     YINLALL"
BASE COUNT         2168 a         2418 c         2569 g         2066 t
ORIGIN      
        1 gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga ccgctaaagc
       61 gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata actcactctg
      121 cgccgtcggc ggtactatta acggtgatga ccatgttttt ggtttatcgg cggcccagcg
      181 ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata tgcgtgagat
      241 gatggcaggc ggcggcaaaa tgatcctcgg gtcagacagc cacacccgtt acggtgcatt
      301 agggacaatg gcagtcggtg agggcggcgg tgagttggta aaacaattgc ttaatgacac
      361 ctgggatatc gactatccgg gagttgttgc ggtgcatctg accggaaaac cagcgccgta
      421 tgtggggccg caggatgtgg cgctggctat catcggtgcc gtgttcaaaa acggctacgt
      481 caaaaacaaa gtgatggaat tcgtaggtcc cggtgttgct gcgctctcta ccgatttccg
      541 taacagcgtt gacgttatga ccactgaaac gacctgttta agttctgtct ggcaaaccga
      601 tgaagaagtc cataactggc tggcgctgca cggtcgcggc caggattact gccagcttaa
      661 ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg ccatcaaacc
      721 aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac tgaaccagaa
      781 cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc acggtaaagc
      841 caaactctcg ctgctggaca aagtggaaaa tggtcgcctg aaagtgcagc aggggattat
      901 cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg cactgcgcgg
      961 tcaatcctgt ggcaatgaca ccttctcgtt agcggtttac ccgtcatcac agccagtatt
     1021 tatggattta gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg caatcatcag
     1081 aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca acggtttgag
     1141 tattcgccac accacgcgta acttcccgaa ccgcgaaggt tctaagccag ccaatgggca
     1201 gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg caaacggtgg
     1261 ctatttaact tctgccagcg aactcgattg ctgggacaac gtgccggagt acgccttcga
     1321 tgtaacgccg tataaaaacc gtgtttatca gggctttgtt aaaggggcaa ctcagcaacc
     1381 gctgatttac ggaccgaaca ttaaagactg gccggaatta ggtgcgctga ctgacaatat
     1441 cgtcctcaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg aactgatccc
     1501 ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt ttaccctgtc
     1561 acgccgcgat ccaggttatg ttggcagaag taaagcgact gctgagctgg aaaatcagcg
     1621 tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc agattgctgg
     1681 tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg cggtgaaacc
     1741 aggcgatggt tctgcgcgtg aacaggcggc gagttgtcag cgtgtgattg gcggtctggc
     1801 gaatattgcc gaggagtacg cgactaaacg ctatcgttct aacgtcatca actgggggat
     1861 gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca tttacatccc
     1921 tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg tgatccatga
     1981 agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg aagagcgcga
     2041 gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt aaaaagcgcc
     2101 atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgctggcg catcttatcc
     2161 gacctacaaa tcgcatcgaa ccgtaggccg gataaggcgt ttacgccgca tccggcaaat
     2221 agttaatagc tcttacttct tcgcctctgc aaccactttg ctacccacgc cgcggttatt
     2281 gtattcccac atgcggttgt agttagtgtc attcagattg cgctgtattt cgtcgctatc
     2341 gtcaacgctg ccggtgttac ccgcaaacgg acgattagag atcaccgcat cgccccacgg
     2401 tttagccgtg ttaaaacctt cgttgatggc gctatcacgg atcaccacct gaccgttggt
     2461 attggcatca acatccagcg agcggcccag ttgtgccacg ccatcaccgg aagcattgaa
     2521 acggctgttt acggcgagga aaccgtagta aatgttggac agcgtagccg gtgcaaacac
     2581 atacgcttct tgctgagtac gtgagttcac cacgcggaat tcggtgttat cgaacaccac
     2641 tgcgccgcga ccagaaacga tatccacatc cccttcaatg tagctgttgg tcaccagcgt
     2701 acgcggctga cgattcgttt ccagacggtt ctgcacaccg ctgttggtga caaagaaggt
     2761 gttctgacga ccgagaatgt taacgttgtt aatctgtacc tggtcaccgt cagtacgcag
     2821 tgccaccgcc ggatggttac ccgcatctac gctatcaccc agcgtgtttt cgatggtcag
     2881 gttttgcagt tgcaggccat tgttttgtga ccagaagacc gcagaacaca gaacaccgat
     2941 actgtcgctg cgtttgctct ggcagctatc gtacatatac cacgctggtt tacctggcat
     3001 atatttgccg cgcgggttga cgtcgtgacg ccagtcggca ggactcatgc ccccatcaag
     3061 ggaaagccca atcttcacat caatcggttt ttcacctgta ccgtacagag taattccacc
     3121 tggagcagca gggacataca ccgttccctg atactcacca ggcatcacgg caatatactg
     3181 gcgcttgttg gtgcgcttga taattgccgc atctaccgcc gcctgaatcg tggtatgcgt
     3241 tacaccttga gtacccgccg ggccgacaac aaagtcaggt tgcgcaggca gggttatcgg
     3301 ggacggattc cacgctgcag cacctggtgt cagggatgca aaatagtgtt gagcatcgaa
     3361 attctgcgct tcttttgccg acagaatcgg gcgagaagag gtaccaggcg cagtttgatc
     3421 agaaggacgt tgatcgggtg gtgttgagct acaggcggtc agcgtcacgc caaaagccaa
     3481 tgccagcgcc agacgggaaa ctgaaaatgt gttcacaggt tgctccgggc tatgaaatag
     3541 aaaaatgaat ccgttgaagc ctgctttttt atactaactt gagcgaaacg ggaaggtaaa
     3601 aagacaaaaa gttgttttta atacctttaa gtgataccag atagcattgc gccatctggc
     3661 agagtaatta actgaacatc gcggtaatcg atgcgcttgc cagagagtgg aaatgaacgt
     3721 taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct acatccagcg
     3781 cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg ccatcgtatc
     3841 cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct accagaccag
     3901 agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact acccagtgcc
     3961 accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa cttttcgttc
     4021 ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg taacccatgc
     4081 cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta ctgatgagtt
     4141 tcataaaccc tcctttctag tttgcagaaa gtgtagccag aaaccctcac gctgacttcc
     4201 cgttattggc aaaaaaatgt ttcatcctgt accgcacgat taacccctgc ggtcagacgc
     4261 tgcaactgtt gcgggtgaat aatatagggc ggcatcaggt aaatcagttt gccaaaaggc
     4321 cggatccaga caccctgttc gacaaagaat ttttgcagcg ccgccatatt caccgggcga
     4381 gtggtttcga ccacgccaat ggcccccagt acgcgcacat cggcaaccat ttcggcatca
     4441 cgggcggggg caagttgctc gcgcagctgc gcttcaatag ccgctacctg atgctgccag
     4501 tcgccagatt cgagaatcgc caggctggcg tttgctgctg cgcaggccag cggattgccc
     4561 ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt ttctgcaacc
     4621 tcgcgcgtgg tgagtgtggc ggaaagggtc attgtgccgc cggttaaggc tttaccgagg
     4681 cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc ggtacgacca
     4741 aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca cattttgcgg
     4801 attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg gacaatcggc
     4861 tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc aaagcccacc
     4921 atatcgcgct catcccattc accatccatg cggctttgcg gggcgggagc aaacaagttt
     4981 tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca caccgacatc
     5041 gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg gcgcgcttcg
     5101 cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc tacggaaccg
     5161 gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca tcgccaccag tttgcggcac
     5221 agctcaatgg ctggcgcatg agtgataccg ccaaacatca catgcgacat ggcatcaatt
     5281 tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc cgcccaccag
     5341 gacgacatac cgtcaaccag gcgtctgcca tcagacaaaa ttagctcgca accttcggcg
     5401 cttgccaccg gataaaccgg taacggggag gtcatggatg tgtatgggtg ccagatatgg
     5461 cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa attgaaaaga
     5521 tttaggttta caagtctaca ccgaattaac aacaaaaaac acgttttgga gaagccccat
     5581 ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa aaccgttgct
     5641 ggatctgctg tttgaagcgc agcaggtcca tcgccagcat ttcgatcctc gtcaggtgca
     5701 ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca aatactgccc
     5761 gcaaagctcg cgctacaaaa ccgggctgga agccgagagg ttgatggaag ttgaacaggt
     5821 gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgtttctgta tgggcgcggc
     5881 gtggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc agggggtaaa
     5941 agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc aagcgcagcg
     6001 ccttgcgaat gccgggctgg attactacaa ccataacctc gacacctcgc cggagtttta
     6061 cggcaatatc atcaccacac gtacttatca ggaacgcctt gatacgctgg aaaaagtgcg
     6121 cgatgccggg atcaaagtct gctcaggtgg cattgtgggt ttaggcgaaa cggtaaaaga
     6181 tcgcgccgga ttattactgc aactggcaaa cctgccgacg ccgccggaaa gcgtgccaat
     6241 caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg ttgatgcctt
     6301 tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt acgtacgcct
     6361 ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta tggctggcgc
     6421 aaactcgatt ttctacggct gcaaactgct gactacgccg aatccggaag aagataaaga
     6481 cctgcaactg tttcgcaaac tggggttaaa tccgcagcaa actgccgtgc tggcagggga
     6541 taacgaacaa cagcagcgtc tggagcaggc actgatgacc ccggatactg acgaatatta
     6601 caacgcggca gcactatgag ctggcaggag aaaatcaacg cggcgctcga tgcgcggcgt
     6661 gctgccgatg ccctgcgtcg ccgttatccg gtgacgcaag gagccggacg ctggctggtg
     6721 gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt aagccatcat
     6781 ccgcaaatta tccgcgcctg gcagcagggc gcagaaaagt ttggtgtagg tagcggcggt
     6841 tccgggcatg tcagcggtta tagcgtggcg catcaggcgc tggaagaaga actggccgag
     6901 tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa tcaggcagtc
     6961 attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag ccatgcctca
     7021 ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttatcca taacgatgtc
     7081 gctcatctgg cgcgactgct tgcttccccc tgttcggggc agcaactggt ggtgacagaa
     7141 ggcgtgttca gcatggacgg cgatagtgcg ccactggcgg aaatccagca ggtaacgcaa
     7201 cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat cggggagcag
     7261 gggcgcggca cctgctggtt gcaaaaggta aaaccagaat tgctggtagt gacttttggc
     7321 aaaggatttg gcgtcagcgg ggctgcggtg ctttgctcca gtacggtggc ggattatctg
     7381 ctgcaattcg cccgccatct tatctacagc accagtatgc cgcccgctca ggcgcaggca
     7441 ttacgtgcgt cactggcggt cattcgcagt gatgagggtg atgcacggcg cgaaaaactg
     7501 gcggcactca ttacgcgttt tcgtgccgga gtacaggatt tgccgtttac gcttgctgat
     7561 tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt acaactggca
     7621 gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac cgtacccgct
     7681 ggtactgcgc gactgcgctt aacactaacc tccgcgcatg aaatgcagga tatcgaccgt
     7741 ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg catttggtcg
     7801 ggcagccgcg cactatgagc aacacgcaga gttacagcgc cagagtgctg acgtcttact
     7861 ggcaatgctc ccacagcgta aatacaccca cgtactggac gcggggtgtg ggccaggctg
     7921 gatgagccgc cactggcgag agcgtcacgc gcaggtgacg gcgttagatc tctcgccgcc
     7981 aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcgg gagatatcga
     8041 atccctgccg ttagcgactg cgacgttcga tcttgtatgg agcaatctcg cggtgcagtg
     8101 gtgcggtaat ttatctacgg cactccgcga gctgtatcgg gtggtgcgcc ccggaggcgt
     8161 ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg cgtggcaggc
     8221 ggtggacgag cgcccgcatg ctaatcgctt tttaccgcca gatgaaatcg aacagtcact
     8281 gaacggcgtg cattatcgac atcatattca gcccatcacg ctgtggtttg atgatgcgct
     8341 cagtgccatg cgttcgctga aaggcatcgg tgccacgcat cttcatgaag ggcgcgacca
     8401 gcgaatatta acgcgttcac agttgcagcg attgcaactg gcctggccgc aacaacaggg
     8461 gcgatatcct ctgacgtatc atcttttttt gggagtgatt gttcgtgagt aaacgttatt
     8521 ttgtcaccgg aacggatacc gaagtgggga aaactgtcgc cagttgtgca cttttacaag
     8581 ccgcaaaggc agcaggctat cggacggcag gttataaacc ggtcgcctct ggcagcgaaa
     8641 agaccccgga gggtttacgt aatagtgacg cgctggcgtt acagcgcaac agcagcctgc
     8701 agctggatta cgcaacggta aatccttaca ccttcgcaga acccacctca ccgcacatca
     8761 tcagcgcgca agagggcagg tcgatagaat catcggtaat gagctccgga ttacgcgcgc
     8821 ttgaacaaca ggctgactgg gtgttagtgg aaggcgctgg cggctggttt acgccgcttt
     8881 ctgacacttt cacttttgcg gattgggtaa cacaggaaca actgccggta atactggtgg
     8941 ttggcgtgaa acttggctgt attaatcacg cgatgttgac ggcacaggcg atacaacacg
     9001 ccgggctgac tctggcgggt tgggtggcga acgatgtcac gcctccggga aaacgtcacg
     9061 ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga gagatcccca
     9121 ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     9181 agccatcctg tgtttggtta aattgcgagc gagatcgcgt c
//
LOCUS       DAZKFU020000188         9212 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZKFU020000188 DAZKFU020000000
VERSION     DAZKFU020000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.190"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..410
                     /locus_tag="ACHOQQ_RS18070"
                     /old_locus_tag="pgaptmp_003614"
     CDS             48..410
                     /locus_tag="ACHOQQ_RS18070"
                     /old_locus_tag="pgaptmp_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471822.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKGMGKTTEE"
     gene            complement(691..1149)
                     /gene="ybbJ"
                     /locus_tag="ACHOQQ_RS18075"
                     /old_locus_tag="pgaptmp_003615"
     CDS             complement(691..1149)
                     /gene="ybbJ"
                     /locus_tag="ACHOQQ_RS18075"
                     /old_locus_tag="pgaptmp_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGU4471823.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1146..2063)
                     /gene="qmcA"
                     /locus_tag="ACHOQQ_RS18080"
                     /old_locus_tag="pgaptmp_003616"
     CDS             complement(1146..2063)
                     /gene="qmcA"
                     /locus_tag="ACHOQQ_RS18080"
                     /old_locus_tag="pgaptmp_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4471824.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2209..2886
                     /gene="fetA"
                     /locus_tag="ACHOQQ_RS18085"
                     /old_locus_tag="pgaptmp_003617"
     CDS             2209..2886
                     /gene="fetA"
                     /locus_tag="ACHOQQ_RS18085"
                     /old_locus_tag="pgaptmp_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGU4471825.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2873..3652
                     /gene="fetB"
                     /locus_tag="ACHOQQ_RS18090"
                     /old_locus_tag="pgaptmp_003618"
     CDS             2873..3652
                     /gene="fetB"
                     /locus_tag="ACHOQQ_RS18090"
                     /old_locus_tag="pgaptmp_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGU4471826.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3715..4569)
                     /gene="cnoX"
                     /locus_tag="ACHOQQ_RS18095"
                     /old_locus_tag="pgaptmp_003619"
     CDS             complement(3715..4569)
                     /gene="cnoX"
                     /locus_tag="ACHOQQ_RS18095"
                     /old_locus_tag="pgaptmp_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGU4471827.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4630..5439)
                     /gene="ybbO"
                     /locus_tag="ACHOQQ_RS18100"
                     /old_locus_tag="pgaptmp_003620"
     CDS             complement(4630..5439)
                     /gene="ybbO"
                     /locus_tag="ACHOQQ_RS18100"
                     /old_locus_tag="pgaptmp_003620"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4471828.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5429..6052)
                     /gene="tesA"
                     /locus_tag="ACHOQQ_RS18105"
                     /old_locus_tag="pgaptmp_003621"
     CDS             complement(5429..6052)
                     /gene="tesA"
                     /locus_tag="ACHOQQ_RS18105"
                     /old_locus_tag="pgaptmp_003621"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGU4471829.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6023..6709
                     /gene="ybbA"
                     /locus_tag="ACHOQQ_RS18110"
                     /old_locus_tag="pgaptmp_003622"
     CDS             6023..6709
                     /gene="ybbA"
                     /locus_tag="ACHOQQ_RS18110"
                     /old_locus_tag="pgaptmp_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGU4471830.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6706..9120
                     /gene="ybbP"
                     /locus_tag="ACHOQQ_RS18115"
                     /old_locus_tag="pgaptmp_003623"
     CDS             6706..9120
                     /gene="ybbP"
                     /locus_tag="ACHOQQ_RS18115"
                     /old_locus_tag="pgaptmp_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGU4471831.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMINLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LSEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMHQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2085 a         2387 c         2549 g         2191 t
ORIGIN      
        1 gataaatctc aatttttaag aggtttacgc caggcaggaa aatttaaatg aatgaatttg
       61 agaagatttt taatgaaatg aatctggacc gggcattgct accgatcctt tttcgttcga
      121 accggtcgac agtgtggaag tatttaagcg gcgatagtac ggctcctgcc tctgccatgt
      181 cgctgattat gctgttgcag ttgatccaga aacgaaaccc cgatctgtta gccgaatggt
      241 taacgctttc tgatttcacc attccgccag aagtttacct ggaccagcct gattactgga
      301 aagggtgggt gtatacgcaa cacaaggtca ataaaaacgt cctggaatat ctaaaaaaac
      361 actatccgga tgaagaccaa aaaggtatgg gtaaaaccac agaagagtaa attgtcatta
      421 cccgcccaca tgccggatgc gcacagactg gcagtgttag taggcatgat aatacgcgtc
      481 agcgtcgcat caggcatcgg cgcataaccg ccggatgcgg cgtaaacgcc ttatccggcc
      541 tacgattcgg gcgcagactt ccaggcatga taatacgcgc ccgcctcgca tcaggtgtcg
      601 gtgcacgact gccggatgcg gcgcaagcgc cttatctggc ctacgattcg ggcatatttt
      661 gtaggcatga taagacgcgt cagcgtcgca tcaggcgatg accgcacgga tgatcagcgt
      721 tatcccttct atcgcaatga cttcaacatg cgtacctgcg ccgagatcct cgctggcgct
      781 gacaggccat gaactgtcac cgacgcgcat atgaccgcgc ccgttgacca gcggagattc
      841 cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg tttaaatgac tgtcgctgtg
      901 cttttgttcg cgcacccgcc gcgacaacca tttccaccac agccaggcgg cgagcagcgt
      961 caggacggca aacatcaccc cttgccactc ccaacccagc ggcaccagcc agaccaccag
     1021 gccggtaatc accgctgcca cgccactcca caacaaataa ccatttccgc ccagcatctc
     1081 ggctgccagc agcaaaccgc cgagactgag ccagaaaata tgtggatgaa cgaccattaa
     1141 ctccatcatg gctgagtccg tttgttggcg ctgtctttca ccagctcagc aatcccggca
     1201 atcgacccca tcaggctgct ggcctctaat ggcatcatca ctactttgct gttactggac
     1261 gaaccgatct gctgtaacgc ttcggtgtat ttctgcgcta cgaagtagtt caccgcctga
     1321 atatcaccgg aggcgatggc ttcagacacc attttggtgg cgcgggcttc tgcttcagcg
     1381 gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac gttcgccttc cgctttcagg
     1441 atttgcgact gtttttcacc ttcggctttg aggatttccg cctgacggat cccttccgct
     1501 tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct gcgcgttcat tgaagagata
     1561 agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg tgactttaat cccccacggg
     1621 ttggtggctt catcgacaat acgcagcagg cgtgagttga tgctgtcgcg ctgagagagc
     1681 atttcgtcaa gttccattga acccagcacg gtacggatgt tagtcatggt caggttgatg
     1741 atcgccagct ccagattgct gacttcatag gcagcgcgtg gcgcgtcaat cacctgaata
     1801 aaacagactg cgtcgatggt aacgttggcg ttatctttcg agataacttc ctgggaaggg
     1861 atatcgagca cttgctccat catattgatc ttgcgaccaa tgcgatccat aaacggcacc
     1921 accagactga gccccggctg taacgttttg gtatagcgac caaaacgttc taccgtccat
     1981 tgatagccct gcggcacgat tttgacgccc gcgccgacaa tgaccagcgc gacaaaaatg
     2041 agaatcggga taaagataag catcggaaaa acctcctgtt gtaccgtcca taatcagcaa
     2101 aattgctgct tgattaaaca aattatacct gattactgaa agagagttcc cccttattcc
     2161 tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg gaacagcgat gcaggaaaat
     2221 agtcctttgc ttcagctaca aaacgtagga tatctggcgg gtgatgcgaa gattcttaat
     2281 aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa ttaccggtcc ttctggttgt
     2341 ggcaaaagta cgctgctaaa aatagttgct tcattgatca gcccaaccag cggaacgtta
     2401 ctgtttgaag gtgaggatgt cagcacacta aagccagaaa tctaccgcca acaagtctct
     2461 tactgcgccc agacaccgac gctgtttggc gatacggtat acgataatct gatctttccc
     2521 tggcagatcc gtaaccggca gcctgacccg gccatttttc tcgattttct tgaacgcttc
     2581 gccttgccgg acagcatttt gacgaagaat atcgccgagc tatctggtgg tgaaaaacaa
     2641 cgcatctcat tgattcgtaa cctgcaattt atgccgaagg ttttattgct ggatgaaata
     2701 accagtgcgc tggatgaaag taataaacat aacgtcaatg agatgatcca tcgttatgtg
     2761 cgcgagcaaa atattgccgt gctgtgggtg acacacgata aagacgaaat taatcatgcg
     2821 gataaagtga ttacactgca accgcatgcc ggagaaatgc aggaagcacg ctatgaactc
     2881 gcataatatt actaacgaat cattagcact ggcattaatg ctggtggtgg tggcaatctt
     2941 aattagccat aaagaaaaac tggcgctgga gaaagatatt ctctggagcg tcgggcgcgc
     3001 gataattcag ctgattattg tcggctatgt gctgaagtat attttcagcg tggatgatgc
     3061 cagcctgaca ttattgatgg tgttatttat ctgctttaat gcggcgtgga acgcgcaaaa
     3121 acgcagtaaa tatattgcta aagcttttat ctcatcgttt attgccatta cggtcggggc
     3181 gggaattacc ctggcggtgc tgattctctc agggtcgatt gaatttatcc cgatgcaggt
     3241 gatccctatc gccgggatga tagccggtaa cgccatggta gcggtagggc tgtgttacaa
     3301 caatttagga caacgggtga ttagcgaaca gcaacagatt caggagaaac tgagtcttgg
     3361 cgcgacgccg aaacaggctt cagcgatatt gattcgcgac agtattcgcg cggctttaat
     3421 tccgacggtc gattcagcaa aaacggttgg cttagtgagt ttaccaggaa tgatgtccgg
     3481 gctgatattt gccgggattg atccggtgaa ggcgattaaa tatcagatta tggtgacctt
     3541 tatgctgctc tcaaccgcca gcttgtcgac cattattgcc tgctatttaa cctatcgtaa
     3601 gttttataat tcgcgccacc agttggtggt gacgcaattg aagaagaaat gatgcgatgc
     3661 cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc cgatcaacgg aatatcaata
     3721 caacaatgca tacagctggc ggcgatactt cgacgccagt gcatcacccg tacccagcgc
     3781 agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct gcggcggtga gatctttacg
     3841 cagatgcccg aacagcaact ccagcgcctc ttcattgcgc ccaacctgat gcagttgcag
     3901 cgccagttgc gtcgccagtg cggcatcttc tggattctcc gccacctgct gttgcaactg
     3961 ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg atttgcgcca ccagcccctg
     4021 gtagcgggtg tcctgatcct gcaacggaat ggttttcagc accgcttccg catcttcaga
     4081 acggttcagc gcaatcagcg tttctgccag cagcaggccg atctccccgt tctgattcga
     4141 caactgccag gcgtctttca gcaatggcag ggcgtcggtg taattgcctt cctgcatcag
     4201 ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc agcactttat ccagcagggc
     4261 gcggatcgcc tcttccggtt gcggcccctg gaagccatct accggttgcc cgttctggaa
     4321 cagatacacg gtcggaatcg cgcgcagacc aaactgggcg gcaatcatct gctccgcgtc
     4381 gcagtccagc ttcgccagaa taaattgccc gttgtactgc gccgcgaggc tttccagaat
     4441 tggggttaac tgcaaacagt gctggctacg ttcagaccaa aaatagaaca gcaccggagt
     4501 ggtcatcgac tgttcaagaa cctgttgcag gttagattcg ttaatgttga caatattttc
     4561 tacggacatg gagtcgctct ctgttgtcga tattttcttt gacatggggg cttaagcgcg
     4621 cgcttcaact cacccctgca atattttgtc catcacgcgc cccggcagca ggcgcttaag
     4681 caccattact gcccaggtca ccagcgttac cggatagcgc atcttcggct tctcgctaat
     4741 aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc aacgtaaagc gggcggcgat
     4801 gccgggattt tcgactggtt tatcactttg cgtctggttg acgttgtcgg tgaagcgagt
     4861 acgaatggga ccgggttcga tcaggctgac tttaattccg ctgtggcgta gctccatgcg
     4921 cagcgcgtct gaccacgcct ccagcgcata tttactggcc gcgtaagcgc cacgacccgg
     4981 cgtggagatt aatcccatca ccgatgatgt catcacaata cgcccttcac cgtgcggtaa
     5041 catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg aaaaagttgg cggaaaactg
     5101 ctgttccatc tgcgcacggc tgatggtgga aagggggcca tacatgccga atccggcatt
     5161 gttaaagatc ccatacagac aattatcggt cagggcgatc acctcgtcgg ctgcgcgatc
     5221 aacactttct ggtgaatcca gatcgatcaa cacgccggta aatcccatgc tgttcatgcg
     5281 ctcaacatca tccggtttcc ggcaacctgc cagcacatga aaaccctggc gttttaattc
     5341 gagcgcgctt tccaggccaa ttccactgga acatccggta attaagaccg atttttgcat
     5401 aactttacct gtcaggatct ccgttgcttt atgagtcatg atttactaaa ggctgcaact
     5461 gcttcgccat ccagtcggca ataaacggct gggcgtcgcg gttgggatga ataccgtcat
     5521 cctgcatcca ttgtggcttg aggtagacct cttccataaa aaagggcagc agcggaacat
     5581 caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc ttcattataa cggcgaccat
     5641 agtttgcagg cagacgtatt tgcattaaca atggttcagc gttggcggct ttgacatcct
     5701 gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg aaaaccacgc aaaccgtcat
     5761 tgccgcccag ttcaaccagc acccagcgcg gctgatgctg tttcagcaga gccggaaggc
     5821 gcgccagccc ttgttgcgag gtgtcgccgc tgatgctggc attgactacc gacgttttac
     5881 tctgccactt atcattcaac aaggcaggcc aggccgcgct ggcagacatt cgatacccgg
     5941 cgctcaggct atcacccaga atcaataacg tgtccgctgc ggcggcacgg aaggttaaca
     6001 ggaccaggaa caggaagggc aaatgccagc ggaaaacatt gttgaagttc atcatcttaa
     6061 gaagtccgtc ggtcaggggg agcatgaact ctccatcctc accggagttg agctggttgt
     6121 caaacgtggc gagaccatcg cactggtggg cgagtcggga tcgggtaagt caaccttgct
     6181 ggcgatcctc gccgggctgg atgacggcag cagtggcgaa gtgagtctgg tgggacaacc
     6241 gctacataat atggacgaag aagcgcgggc aaagttgcgc gcgaagcacg tcggctttgt
     6301 ttttcagtca tttatgttaa ttcctaccct taacgcgctg gaaaacgtcg agcttccggc
     6361 gctgctgcgc ggtgagagta gcgcggaaag tcgtaacgga gcgaaagcgt tgctcgaaca
     6421 gttagggctg ggtaaacgtc tggatcatct tccggcacag ctttccggcg gtgaacagca
     6481 acgagtggcg ctggcacgag cctttaacgg tcgacctgat gtgctgtttg ccgacgaacc
     6541 caccggtaac cttgaccgcc agacgggcga taaaattgcc gacttgctgt tttccctcaa
     6601 ccgtgagcat ggcaccacgt tgataatggt gacccacgac ctgcaactgg cggcacgctg
     6661 cgaccgctgc ttacggctgg tgaacgggca gttgcaggag gaagcatgat tgcacgttgg
     6721 ttctggcgcg aatggcgctc gccgtcgcta ttaattgtct ggctggcgct aagcctggcg
     6781 gtggcctgcg tgctggcgct gggcaatatc agcgatcgca tggagaaggg cttaagccag
     6841 caaagccgtg agtttatggc gggcgatcgg gcgctgcgca gttcgcgcga agtgccgcaa
     6901 gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg gcaagcagct gactttcgcc
     6961 accatgacat ttgcaggcga cacaccgcag ctggcgaacg tcaaagcggt ggatgatatc
     7021 tacccgatgt atggcgatct gcaaaccaat ccccctggcc tgaaaccgca ggcgggcagc
     7081 gtattgctgg ccccacgcct gatggcactg cttaacctga aaacgggcga caccattgac
     7141 gtgggcgatg ccaccttgcg gattgccgga gaagtgattc aggaaccgga ttccggtttt
     7201 aaccccttcc agatggctcc gcgtctgatg attaatctgg cggatgtcga taaaaccgga
     7261 gccgtgcagc cggggagtcg ggtcacctgg cgctataaat tcggcggcag cgagaaccag
     7321 ctcgacagct atgagaaatg gttgttacct cagcttaaac ccgaacaacg ctggtacggt
     7381 ctggaacagg acgaaggcgc gctggggcga tcgatggaac gctcgcagca gttcctgctg
     7441 ctttcggcgc ttctgacctt gctgctggca gtggcagcgg tggcggtggc gatgaatcat
     7501 tactgtcgca gtcgctacga tctggtggcg atcctcaaaa cgctgggggc agggcgagcg
     7561 caactgcgta agctaatcgt cggtcagtgg ttgatggtgc tgacgctttc agccgttacc
     7621 ggtggggcga taggcctgtt gttcgaaaac gtgttgatgg tgctgctcaa gcccgttctg
     7681 cctgctgcac taccgccagc cagcctctgg ccatggctgt gggcgctcgg caccatgacg
     7741 gtcatctcgc tgctggtggg gctacgacca taccgcttgt tgctggcaac gcagccttta
     7801 cgcgtattac gtaatgatgt ggttgcgaac gtctggccgc tgaagtttta tctgccgatt
     7861 gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg gtggcagcat gctgctttgg
     7921 gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg gtgtgctggg ctggatgctg
     7981 ctgaatgtac ttcgccgtat gacgctgaaa tcgctgcctc tgcgcctggc ggttagccgc
     8041 ctgttacgtc agccgtggtc aacgttaagc cagctttcgg cattttcgct ctcctttatg
     8101 ctgctggcac tgctgctggt gttgcgtggc gatctgctcg accgctggca gcagcagcta
     8161 cctccagaaa gcccgaacta ctttttaatt aacatcgcca cagaacaggt agcaccgcta
     8221 aaagcgttcc tctcggaaca tcagatagtc ccggaatcgt tttatccggt ggtgcgggcg
     8281 agactgacgg cgattaacgg caagccgaca gaaggcaatg aagatgaggc gctcaatcgc
     8341 gaactcaatc ttacctggca aaatacgcgg cccgatcata atccgattgt cgccggtaac
     8401 tggccgccaa aagccgatga agtgtcgatg gaagaggggc tggcgaaacg cttaaacgtt
     8461 gccctcggtg ataccgtgac ttttatgggc gatacccagg agttccgcgc taaagtgacc
     8521 agcctgcgca aagttgactg ggaaagtctg cggcctaatt tctattttat tttccctgaa
     8581 ggggctctgg acgggcaacc gcagagctgg cttaccagtt tccgctggga gaatggcaac
     8641 ggcatgttga cgcaactcaa ccgccagttc ccgaccatta gcctgttaga cattggcgcg
     8701 attttaaaac aggtcggcca ggtgctggag caggtaagtc gggcgctgga ggtgatggtg
     8761 gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac aggtgcaggt gggaatgcat
     8821 cagcgtcatc aggagctggt ggtgtggcgc acactcggtg cggggaaaaa actgctgcgc
     8881 acgacattgt ggtgcgagtt cgccatgctt gggtttgttt ctggcctggt ggccgcaatt
     8941 ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt ttgatttccc gtgggagcca
     9001 gactggcgat tgtggattgt tctgccgtgc agcggtgcgc tgctgctgtc gcttttcggc
     9061 ggctggctgg gcgcgagact ggttaagggt aaggcgctgt tcaggcagtt tgcggggtga
     9121 tgagagtaaa taaggggcgt tctggttgaa tggaacgcct tgtttaaatt aattctacaa
     9181 tatgtttcat tgatgatgtt attgaattgg tg
//
LOCUS       DAZKFU020000189         9165 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZKFU020000189 DAZKFU020000000
VERSION     DAZKFU020000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.191"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(106..1326)
                     /gene="fsr"
                     /locus_tag="ACHOQQ_RS18120"
                     /old_locus_tag="pgaptmp_003624"
     CDS             complement(106..1326)
                     /gene="fsr"
                     /locus_tag="ACHOQQ_RS18120"
                     /old_locus_tag="pgaptmp_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGU4471832.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            1544..3196
                     /gene="ushA"
                     /locus_tag="ACHOQQ_RS18125"
                     /old_locus_tag="pgaptmp_003625"
     CDS             1544..3196
                     /gene="ushA"
                     /locus_tag="ACHOQQ_RS18125"
                     /old_locus_tag="pgaptmp_003625"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGU4471833.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARVLPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QLDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="ACHOQQ_RS18130"
                     /old_locus_tag="pgaptmp_003626"
     CDS             complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="ACHOQQ_RS18130"
                     /old_locus_tag="pgaptmp_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGU4471834.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3916..4710)
                     /gene="ybaP"
                     /locus_tag="ACHOQQ_RS18135"
                     /old_locus_tag="pgaptmp_003627"
     CDS             complement(3916..4710)
                     /gene="ybaP"
                     /locus_tag="ACHOQQ_RS18135"
                     /old_locus_tag="pgaptmp_003627"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGU4471835.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTNDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4848..5189
                     /gene="ybaQ"
                     /locus_tag="ACHOQQ_RS18140"
                     /old_locus_tag="pgaptmp_003628"
     CDS             4848..5189
                     /gene="ybaQ"
                     /locus_tag="ACHOQQ_RS18140"
                     /old_locus_tag="pgaptmp_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4471836.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLALKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5404..7908)
                     /gene="copA"
                     /locus_tag="ACHOQQ_RS18145"
                     /old_locus_tag="pgaptmp_003629"
     CDS             complement(5404..7908)
                     /gene="copA"
                     /locus_tag="ACHOQQ_RS18145"
                     /old_locus_tag="pgaptmp_003629"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGU4471837.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADSDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            8170..9102
                     /gene="glsA"
                     /locus_tag="ACHOQQ_RS18150"
                     /old_locus_tag="pgaptmp_003630"
     CDS             8170..9102
                     /gene="glsA"
                     /locus_tag="ACHOQQ_RS18150"
                     /old_locus_tag="pgaptmp_003630"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGU4471838.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         2274 a         2412 c         2413 g         2066 t
ORIGIN      
        1 tgcaaaaaaa gcacgaatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg
       61 actgtaaaac gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg
      121 gttatcaggc aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata
      181 gactaactcg atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc
      241 catgccaaaa gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagcag
      301 ctcctgagcg taaaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac
      361 cgttaaaacc cccgtccagt gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac
      421 gccgaggata gagccccaaa tcacatattt ccgcccaatt ttatccccta caggcccgcc
      481 gatcaccgta cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg
      541 gatagataat ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat
      601 atagaaatat ttcgagaaaa tgaggattaa cagaatgctg accgccagta ccactttgtt
      661 gcgcggcagt ggattgataa tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc
      721 cgagtaccaa cggctgattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa
      781 ccaggcaacg ttgcctttgc cataaggcgc gataatcacc gccgccagca agggtcccag
      841 ggaactgcca aagttgccgc cgacctgaaa gatagattgt gccaggccat gccgcccgcc
      901 ggaagccata cgggccacgc gagaagattc cggatgaaag accgatgaac cggtaccgac
      961 cagcgccgcc gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc
     1021 acttaaggta aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca
     1081 gtagccgacc actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc
     1141 aatctgcata aatgtcagag aaaactctga ctgaagcagc ggataaatcg ccagaatcag
     1201 cgattggatc atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga
     1261 tgttcgggcc ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat
     1321 tgccatagga aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta
     1381 ttacccgact aacatacctg tatgcgccgc ctgaaggaag tctcaacgct gaatacagaa
     1441 tttctaatct ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc
     1501 atactatttt tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg
     1561 gggcgtggcg ttagcactgt taaccacatt tacactggcg agtgaagctg ctctggcgta
     1621 tgagcaggat aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt
     1681 ttggcgcaat gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat
     1741 ccgcaaagag gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa
     1801 cactggcgtg cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct
     1861 ggtgggctat gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt
     1921 acgtcagcag gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag
     1981 taccggcgag cgcctgttta aaccatgggc gctgtttaag cgtcaggatc tgaaaattgc
     2041 cgttattggt ctgacgacgg atgacacagc gaagttaggc aacccagaaa acttcaccga
     2101 tattgagttc cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac
     2161 agaaaagcca gacattatta tcgcggcgac ccatatgggg cattacgaca atggtgagca
     2221 cggctctaat gcaccgggcg atgtggagat ggcacgcgtg ctgcctgccg gatcgctggc
     2281 gatgatcgtc ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca
     2341 ggtcgattac gtgccgggta cgccatgcaa accggatcaa caaaacggca tctggattgt
     2401 gcaggcacat gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga
     2461 aatgaaaatg gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga
     2521 agacgggaaa agcgagcgcg tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat
     2581 ctcgctgtta tcgccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcga
     2641 aaccaatggt cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg
     2701 gcggttaatt ctggcagcac aattggatcg cactggtgcc gactttgcgg tgatgagcgg
     2761 aggcggaatt cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt
     2821 gcagccattc ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta
     2881 cctgaccgcc gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt
     2941 tagctttgtg gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga
     3001 tccagcgaaa acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata
     3061 tccgcgcctt gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct
     3121 gaaagcgtat atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga
     3181 ggtgagctgg cagtaatccg aaagtgccgg atgttcgcat ccggcacaat gcttaatcgc
     3241 ggcgggcgat atcagcaaat ttggcatcga ggatcttcgc cagatcgcct gccgccagtt
     3301 cgatatccag tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg
     3361 cgtcgataat cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt
     3421 atcccgtcga acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg
     3481 cttttgctac ttttttaagg tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt
     3541 gtttcatatc accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac
     3601 ctaatttttt gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat
     3661 ggatttgaaa cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt
     3721 tcctgcctta aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag
     3781 tcatcttgcc taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt
     3841 cgcttaaagt gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg
     3901 atattggcca tttttttagc gcaacatttg cggtaaattc ccttctccat acaggtgtag
     3961 tgcaccgacc gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc
     4021 gagatttcgc tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt
     4081 tatttcatta ttttgcggcg gcgcattcag ccaccagctc atcatttgtt gcagcaaccg
     4141 tgcattggtg tgccagtggg tcagcgtatc gtccagcagt gccagtcctt tgtcagggag
     4201 ctggagcaac atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg
     4261 ttgcttcgcc gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt
     4321 ttgcgcctgc gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag
     4381 tgagggagaa atgcccatct cctggctaat gtgctgcagt ttttggagtt gctcctcgct
     4441 aatgcgctct tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgttggtgga
     4501 aacatccgcc tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg
     4561 caggggagcc atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt
     4621 gccgggaagg gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca
     4681 aagcgttttt acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag
     4741 cgcgcggtga gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc
     4801 agtatgttct tgtatcgcta ttcacaggga agcaacagtt aaaaactatg aaacaggcaa
     4861 caagaaaacc gacgacaccc ggagatattc ttctctatga atatctggaa ccgctcgctt
     4921 tgaaaatcaa tgagttagca gagttgctgc atgttcatcg taatagcgtc agtacactga
     4981 tcaataataa tcgtaaactc accactgaaa tggcattccg tctggcgaaa gtttttgata
     5041 ccacagtcga tttttggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca
     5101 acatgcgtac ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc
     5161 gtgaagagcg tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg
     5221 ataaggcgtt tacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg
     5281 tcttatcagg cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt tcacgccgca
     5341 tccggcagtc atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg
     5401 tacttattcc ttcggtttaa accgcagcaa ccggttggcg ttactcacta cggtaatcga
     5461 cgagagcgcc attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca
     5521 caaaataccg gcggcgaccg gaataccgat actgttgtag ataaacgcgc cgagcaggtt
     5581 ctgtttcatg ttgcgcagcg ttgcgcggga aatagcgagc gcatccgcaa cgcccatcag
     5641 gctatggcgc atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccccccat
     5701 cgcaatgccg acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat
     5761 tgccacctga cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag
     5821 caccccggct atcacctcat caatcccggc ttctttggcg atcgcattgg cggtggttgg
     5881 gttatccccg gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagtgccgc
     5941 cacgctatca ctacgcaatg gatcgcgtac agccagcagg gctactgctt tcccgtcaat
     6001 cgccagcagc acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc
     6061 tttagtatcg acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc
     6121 ttctgcttca ccgctcacac ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag
     6181 ctgcatatcg cttgctttat cgaggatcgc ccgtgccagc ggatggctgg aaccttgttc
     6241 cagtgccgcc gccagacgca gcgcctgcgc ttcgtcagaa tcagcaaatg ttttcactgc
     6301 gacaacctgc ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag
     6361 tgtactggcg cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg
     6421 cccgacgccg gaaataatcg acatcggcgt cgccagcccc agcgcacacg gacaggcaat
     6481 aatcagcacc gtggtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa
     6541 ataccagatt gccgcactga caagcgcaat aaccactact accggcacaa atacggctga
     6601 gattttatcc gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatacg
     6661 aataatcctt gatagcgtgg tatggctgcc aaccgcactg gcgcgaaaca gaacgctacc
     6721 gtcctgtacc actgtcccgg catggacgct atcgccttcg cctttttgct gcgggatcgg
     6781 ttcgcccgtc agcatcgctt catccagcca cgcttcgccc tgggtaatct cgccatctac
     6841 cggcacgcgg tcgccggtcg tcaggcgcag tagcatgcct ggctgaactt ccgccagcgg
     6901 cacgcttttt tcaccttcgt cggtgaccag gcgcgccgtc ggcggagtta aatcgagtaa
     6961 tttctccagc gcctttgaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt
     7021 gatcagaccg ataatcatcg cgctggcttc gtaataaaga tgtcgcgctt ccatcgggaa
     7081 ccactgcggc cacaggttga cgctcatcga atagagccac gccacgccag tacccagcgc
     7141 caccagcgta tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa
     7201 atggccgccg gcgaaaacca tcactgccag ggttatcagg ccgataacta accacaagct
     7261 gcggttgtca gcggtgacca tcatgttatc gccgatcatc ccccagacca ttaccgggat
     7321 acccacagcc agtgcaacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt
     7381 ttcttgctgg cgctcgcggc gtttcgcgtc atcttcaatc gcttccgcac cgtagcccgc
     7441 tttttccacc gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg
     7501 ctccgccagg tttacccgtg cctgagtgac gcccggtacg ctttgcaacg cattttgtac
     7561 gcgggtgaca cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc
     7621 atcggcggtc gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga
     7681 tgactccgcc agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt
     7741 gatggtttcg atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga
     7801 cacatccgct tgttccacat ccgggcgctg ttcgagactt tctttcacgc gtttaacgca
     7861 gtgaccgcag gacaggccgt ccagggtcag gtcgatagtt tgtgacataa aacactcctt
     7921 taagacagtt ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga
     7981 aattaataac ccaggcgagt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc
     8041 aggattagac atttatctct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc
     8101 aggatgcctg aaaatcggca ccggggtgag gaattacctc ccgcatctat aaaaaggagt
     8161 taacaaaaga tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa
     8221 tttcactcac ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtgcct
     8281 ggtcaactgg cagcagtggc tatcgtgacc agcgatggca acgtctatag tgcgggtgac
     8341 agtgattacc gctttgcgct ggaatccatc tcgaaagtct gtacgttagc ccttgcgtta
     8401 gaagatgtcg gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc
     8461 tttaactcag ttatcgcctt agagttgcat ggcggcaaac cgctgtcgcc actggtaaat
     8521 gctggcgcta ttgccaccac cagcctgatt aacgctgaaa atgccgaaca acgctggcag
     8581 cgaattttac atatccaaca gcaactggct ggtgagctgg tagcgctctc tgacgaagtc
     8641 aaccagtcgg aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc
     8701 gccggatatc tctattgtga tgcaatggaa gcatgtgacg tgtacacccg tcagtgctcc
     8761 acgctcatca atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg
     8821 aatccgttga cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa
     8881 atgatgatgg aagggctgta tggtcgctcc ggtgactggg cgtatcgtgt tggtttaccg
     8941 ggcaaaagcg gtgtaggtgg cggtattctg gcggtcgtcc ctggagtgat gggaattgcc
     9001 gcgttctcac caccgctgga cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca
     9061 tcggtcgcta agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag
     9121 gtaataacgg taacaaaccc ctcggtctat ggaacgtcgt ttcca
//
LOCUS       DAZKFU020000190         9153 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZKFU020000190 DAZKFU020000000
VERSION     DAZKFU020000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.192"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            19..93
                     /gene="tnaC"
                     /locus_tag="ACHOQQ_RS18155"
                     /old_locus_tag="pgaptmp_003631"
     CDS             19..93
                     /gene="tnaC"
                     /locus_tag="ACHOQQ_RS18155"
                     /old_locus_tag="pgaptmp_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HGU4471839.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            314..1729
                     /gene="tnaA"
                     /locus_tag="ACHOQQ_RS18160"
                     /old_locus_tag="pgaptmp_003632"
     CDS             314..1729
                     /gene="tnaA"
                     /locus_tag="ACHOQQ_RS18160"
                     /old_locus_tag="pgaptmp_003632"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGU4471840.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1820..3067
                     /gene="tnaB"
                     /locus_tag="ACHOQQ_RS18165"
                     /old_locus_tag="pgaptmp_003633"
     CDS             1820..3067
                     /gene="tnaB"
                     /locus_tag="ACHOQQ_RS18165"
                     /old_locus_tag="pgaptmp_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGU4471841.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3199..4374
                     /gene="mdtL"
                     /locus_tag="ACHOQQ_RS18170"
                     /old_locus_tag="pgaptmp_003634"
     CDS             3199..4374
                     /gene="mdtL"
                     /locus_tag="ACHOQQ_RS18170"
                     /old_locus_tag="pgaptmp_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGU4471842.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSQAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4349..5308
                     /gene="yidZ"
                     /locus_tag="ACHOQQ_RS18175"
                     /old_locus_tag="pgaptmp_003635"
     CDS             4349..5308
                     /gene="yidZ"
                     /locus_tag="ACHOQQ_RS18175"
                     /old_locus_tag="pgaptmp_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGU4471843.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPSGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5465..6214
                     /gene="yieE"
                     /locus_tag="ACHOQQ_RS18180"
                     /old_locus_tag="pgaptmp_003636"
     CDS             5465..6214
                     /gene="yieE"
                     /locus_tag="ACHOQQ_RS18180"
                     /old_locus_tag="pgaptmp_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGU4471844.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6236..6802
                     /gene="yieF"
                     /locus_tag="ACHOQQ_RS18185"
                     /old_locus_tag="pgaptmp_003637"
                     /gene_synonym="chrR"
     CDS             6236..6802
                     /gene="yieF"
                     /locus_tag="ACHOQQ_RS18185"
                     /old_locus_tag="pgaptmp_003637"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGU4471845.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQEEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTNSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6856..8193)
                     /gene="adeP"
                     /locus_tag="ACHOQQ_RS18190"
                     /old_locus_tag="pgaptmp_003638"
     CDS             complement(6856..8193)
                     /gene="adeP"
                     /locus_tag="ACHOQQ_RS18190"
                     /old_locus_tag="pgaptmp_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGU4471846.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8360..9025
                     /gene="yieH"
                     /locus_tag="ACHOQQ_RS18195"
                     /old_locus_tag="pgaptmp_003639"
     CDS             8360..9025
                     /gene="yieH"
                     /locus_tag="ACHOQQ_RS18195"
                     /old_locus_tag="pgaptmp_003639"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGU4471847.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGINVDLEEIFT
                     RFKGVKLYEIIDIISKEHGVTMVQADAEHVYRAEVARLFDTELADIAGANELLASVNV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
BASE COUNT         2244 a         2288 c         2298 g         2323 t
ORIGIN      
        1 ttcgaggata agtgcattat gaatatctta catatatgtg tgacctcaaa atggttcaat
       61 attgacaaca agattgtcga tcaccgccct tgatttgccc ttctgtagcc atcaccagag
      121 ccaaaccgat tagattcaat gtgatctatt tgtttgctat atcttaattt ttccttttgc
      181 aaaggtcatc tctcgtttat ttacttgtgt tagtaaatga tggtgcttgc atatatacat
      241 ggcgaattaa tggatattgc aaatgtaata ttcacaggga tcactgtaat taaaataaat
      301 gaaggattat gtaatggaaa actttaaaca tctccctgaa ccgttccgca ttcgtgttat
      361 tgagccagta aaacgtacca ctcgcgctta tcgtgaagag gcaattatta aatccggtat
      421 gaacccgttc ctgctggata gcgaagatgt gtttatcgat ttactgaccg acagcggcac
      481 cggggcggtg acgcagagca tgcaggctgc gatgatgcgc ggcgacgaag cttacagcgg
      541 cagccgcagc tactatgcgt tagccgagtc agtgaaaaat atctttggtt atcaatacac
      601 cattccgact caccagggcc gtggcgcaga gcaaatctat attccggtac tgattaaaaa
      661 acgcgagcag gaaaaaggcc tggatcgcag caaaatggtg gcgttctcta actatttctt
      721 tgataccacg cagggccata gccagatcaa cggctgtacc gtgcgtaacg tctatatcaa
      781 agaagcattc gatacgggcg tgcgttacga ctttaaaggc aactttgacc ttgagggatt
      841 agaacgcggt attgaagaag ttggtccgaa taacgtgccg tatatcgttg caaccatcac
      901 cagtaactcc gcaggcggtc agccggtttc actggcaaac ttaaaagcga tgtacagcat
      961 cgcgaagaaa tacgatattc cggtggtaat ggactccgcg cgctttgctg aaaacgccta
     1021 tttcatcaag cagcgtgaag cagaatacaa agactggacc atcgagcaga tcacccgcga
     1081 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat
     1141 gggcggcttg ctgtgcatga aagacgacag tttctttgat gtgtacaccg agtgcagaac
     1201 cctttgcgta gttcaggaag gcttcccgac atatggcggc ctggaaggcg gcgcgatgga
     1261 gcgtctggcc gtaggtctgt atgacggcat gaatctggac tggctggctt atcgtattgc
     1321 gcaggtgcag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
     1381 cggtcacgcg gcattcgttg atgccggtaa actgttgccg catatcccgg ctgatcaatt
     1441 cccggcacag gcgctggcct gcgagctgta taaagtcgcc ggtatccgcg cggtagaaat
     1501 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggcaga
     1561 actactgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
     1621 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaagggttaa cctttaccta
     1681 cgaaccgaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca
     1741 gagtggctat aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc
     1801 ttcctctaaa ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg
     1861 tgttatggtt atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct
     1921 tgccggtgcc tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct
     1981 tcattccggg ttattgttat tagaagcaaa tttaaattat cccgtcggct ccagttttaa
     2041 caccatcacc aaagatttaa ttggtaacac ctggaacatt atcagcggta ttaccgttgc
     2101 cttcgttctc tatatcctca cttatgccta tatctctgct aatggtgcaa tcattagtga
     2161 aacgatatca atgaatttgg gctatcacgc taatccgcgt attgtcggga tctgcacagc
     2221 cattttcgtt gccagcgtat tgtggataag ttcgttagcc gccagtcgta ttacctcatt
     2281 gttcctcggg ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt
     2341 cgattactcc attctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta
     2401 tatctttatg gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag
     2461 cctgattatt tgctatggca aacgcaaaga taagctaatc aaaagcgtgg tcttcggttc
     2521 gctgctggcg ctggtgattt atctcttctg gctctattgc accatgggga atattccgcg
     2581 agaaagcttt aaggcgatta tttcctcagg cggcaacgtt gattcactgg tgaaatcgtt
     2641 cctcggcacc aaacagcacg gcattatcga gttttgcctg ctggtgttct ccaacttagc
     2701 tgtcgccagc tcgttttttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt
     2761 taagattgat aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc
     2821 cgcgttgttg tatctgatct tcccgaatgg ctttatttac gggatcggcg gtgccgggct
     2881 gtgcgccact atctgggcgg tcattatccc cgcggtgctg gcaataaaag ctcgcaaaaa
     2941 gtttcccaat cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct
     3001 ctttggtata accgtaattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt
     3061 tggctaaatc cttcaagaag ccagtcattc gctggcttct tgcctctcag gaaatcactt
     3121 atgtccaaat ggcaactcgc ctgatcctcc ttcaccacgt atgctttgcg tcaccttact
     3181 atcaggacgc tttagcccat gtcccgcttt ttgatttgta gttttgccct ggttttactt
     3241 tatcccgctg ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat
     3301 gccagcgaag cgcagttaca tattgcgttc tccgtttatc tggcaggtat ggcagctgcg
     3361 atgttatttg ccggtaaagt ggccgatcgt tcagggagaa aaccggtcgc catccccggc
     3421 gcggcgctat ttattatcgc ttcggtgttc tgttcacagg ctgaaaccag tacgttattt
     3481 cttgcagggc gctttctaca ggggttgggc gcgggctgtt gttacgtggt ggcattcgcc
     3541 attttgcgcg acacgctgga tgatcgacgt cgggcaaaag tgctgtcgtt actcaacggc
     3601 attacctgca tcattccggt gttagcgccg gtgctcggtc atctgattat gcttaaattc
     3661 ccgtggcaga gtctgttctg gacgatggca ataatgggca tcgcggtact gatgttgtct
     3721 ttgtttattt taaaagaaac gcgccctgcc gcccccgcag cttcggacaa atcacgagaa
     3781 aatagcgagt cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc
     3841 gtttcggtga tcctcacctt cgtcaacacg tcgccggtat tgctgatgga aatcatgggt
     3901 tttgagcgcg gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt
     3961 tcattctcga cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctca
     4021 caggtgttat tcctggcggc ggggatcact cttgccgttt caccttccca tgcggtttct
     4081 ctgtttggta tcacgctgat ttgcgccggt ttctcggttg gttttggtgt ggcgatgagt
     4141 caggcgttag ggccgttttc attacgcgcg ggtgtagcca gctcgacctt aggtattgcg
     4201 caggtttgcg gttcgtcact gtggatttgg ctggcggcgg tggttggtat cggcgcatgg
     4261 aatatgctga tcgggattct gattgcatgt agcatagtga gcctgttgct gattatgttc
     4321 gtcgcgcctg gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa
     4381 tctgctgctc tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg
     4441 gatgaacgtg accccttcgg cggtgagtaa gtcgctggca aagttaagag cgtggtttga
     4501 cgacccgctc tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat
     4561 ggagcaaaat ctggcggagt ggatgcaaat gagcaacctg ctgctggata aaccgcatca
     4621 ccagacaccg agcggcctaa agtttgagct ggcggcagaa tcaccgctaa tgatgatcat
     4681 gcttaatgcg ctgtcgaaac agatctacca acgttacccg caggcgacca tcaaattacg
     4741 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata tcggtttttc
     4801 cggtcgcgaa agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga
     4861 ttatgaagtg ctgtttagtg atgtgccttg cgtctggtta cgcaaagatc atccggcact
     4921 gcatgaaacg tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga
     4981 acagagcgat acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat
     5041 tgctatgagc ctgccggaat tcgagcagtc gctgtttatg gcagcgcagc ccgacaatct
     5101 gctactggcg accgcgccgc gctactgtca gtactacaat caactccacc aactgccgtt
     5161 ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag tcccttttac
     5221 cctgctgtgg cacaaacgga acagccataa tccgaagatc gtctggttac gggaaaccat
     5281 taaaaatctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc ctatttcaca
     5341 ataaaatgta aaaaatttgt aataaacttg tctgaatcga acttttagcc gctttagtct
     5401 gtccatcatt ccagtaaatg attactcttg tattcataat ggaccattaa gcatggagcg
     5461 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     5521 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt
     5581 tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     5641 gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agcgataaaa atttgccttc
     5701 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     5761 tggcctcgat atggaactgc agcgtgcgac acgcgggttt catagcccac acgcgcccga
     5821 taaccacacc ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga
     5881 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     5941 gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca
     6001 tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt
     6061 cacgcccacg attgaaaagc tcagtgtctg ggagttagat ggcaaacacg gctggaaaag
     6121 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     6181 gctctctacc atgaaggctt tttcgccaaa ttgatagaca accacaggag tcattatgtc
     6241 tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     6301 ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     6361 cattgccgac attcccctgt atgacgctga cgtacaggag gaagaaggtt ttccagcaac
     6421 ggttgaagct ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga
     6481 atataactat tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     6541 ggatcaaccg ctggccggta aaccggtgtt gattcagacc aactcaatgg gcgtgattgg
     6601 cggcgcgcgc tgtcagtatc acctgcgtca gattctggtt ttcctcgatg caatggtgat
     6661 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatccgc aaaccggaga
     6721 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt
     6781 tattcagcga gttaagatct aaataaaaaa cccgccagca atcatgcatg gcgggttttt
     6841 aatgcgctat cgattttaat gagcgtcgat aaatacaatc ttcaggataa acagcagcgc
     6901 aacgatgatt acgcacgggc taaggtcacg gagacgcccg gtaccaatct tcatcacgca
     6961 gtaggagata aagcccagcg caataccttc ggtaatcgag aagctgaacg gcatcatcac
     7021 agcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact
     7081 tgaggtcatc aacacgccaa cgtaaatcag cgcgccagct gcagcgtagc ctggcaccat
     7141 tcccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     7201 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     7261 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt
     7321 catgcgcggg aatttcccct tctcatctgc cagacctgct ttatcggtca cgccaatcaa
     7381 cgtaccggag gagtcaaaca ggttgaccag catgaaagag aaaatcaccc ctgccagccc
     7441 gaggttaaac gacccggcta aatctacatg acccacgaca gtcattacgc tcggcggcgc
     7501 agaaacgatg ccattgtagt ggacatcacc cagcatccag cccagcagcg ttgtcaccac
     7561 gatagaaacc agcaccgctg cgtgaatgtt gcgtgaggcc agaatagcaa tgatgaagaa
     7621 gccgaggatc cccagcagca cgctgtgaga agtcagatta ccgatgctga ccagcgtttc
     7681 cgggttagcg acaatcacac ctgcgttttt cagccccatc atgccaatga acagaccgat
     7741 accgctggta atgcccacac gcagactcac cggaatgttg gcaatcatcc agtagcgaac
     7801 gcggaaaatc gtcagtaata gcagaccaat cgcgccccag aagattgcgc ccatcccgac
     7861 ttgccacggc aagcccatcg cctgcacaac gacaaaagcg aagaacgcat tcaggcccat
     7921 agcgggtgcc agtgcaactg gcaggttagc aaacagcccc atcataatac tgccgaatgc
     7981 agcaatcaga caggtagtga cgaagacggc gctggtatcc atgccagcaa cgccaagaat
     8041 ttgcgggtta acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac
     8101 ttcggtccgt gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg
     8161 gccagaagcc tgggttgtgt gttgttgact cattatctat ttccgaacaa ggagggaaaa
     8221 tccgtcgcta tcgtatacca aaatgagaca atatggacgg ctgaagataa ctttttttca
     8281 ttgtatttgc ttatacggca acgattgcgt catgcgaatt ggcatgacga aaacgttaaa
     8341 ctaacgataa aggaaagaca tgtcccagat agaagcggta tttttcgact gcgacggtac
     8401 gctggtcgat agcgaggtca tttgctctcg cgcctatgtc acgatgttca gggaatttgg
     8461 cattaacgtt gacctggagg agatttttac ccgttttaaa ggcgtaaaac tctacgaaat
     8521 catcgacatc atcagtaaag aacatggcgt gacgatggtc caggccgatg ccgagcacgt
     8581 ttaccgcgca gaagtagcgc gtctgtttga taccgaactt gcggacatcg caggcgcgaa
     8641 cgaattgctg gcgagcgtta acgtgccgat gtgcgtcgtc tctaacggtc cggtcagcaa
     8701 aatgcagcat tcgctgggta agctcaacat gctgcactac ttcccggaaa aactgttcag
     8761 cggctacgat atccagcgtt ggaagcccga tccggcatta atgttccatg cggcaaaagc
     8821 gatgaatgtg aacgttgaaa actgcattct ggtcgatgac tccagcgctg gcgctcaatc
     8881 agggatagat gccggaatgg aagtgtttta cttctgcgct gacccgcaca ataagccgat
     8941 cgttcacccg aaagtcacca cctttaccga gctgtcgcag ctgcctgcgc tctggaaggc
     9001 acgcggctgg aatataacgc gataatcgcc tccttacttc aacgcttaca catcagtcac
     9061 gctggtttga gataaagaac accaggtgtc tgatgagtaa gcgtctattc cctatgaata
     9121 aattatgtcg ttattcgtcc tgtcatccac cct
//
LOCUS       DAZKFU020000191         9123 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZKFU020000191 DAZKFU020000000
VERSION     DAZKFU020000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.193"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            169..450
                     /gene="drpB"
                     /locus_tag="ACHOQQ_RS18200"
                     /old_locus_tag="pgaptmp_003640"
     CDS             169..450
                     /gene="drpB"
                     /locus_tag="ACHOQQ_RS18200"
                     /old_locus_tag="pgaptmp_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HGU4471848.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            490..1185
                     /gene="yedJ"
                     /locus_tag="ACHOQQ_RS18205"
                     /old_locus_tag="pgaptmp_003641"
     CDS             490..1185
                     /gene="yedJ"
                     /locus_tag="ACHOQQ_RS18205"
                     /old_locus_tag="pgaptmp_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HGU4471849.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALDVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPHTMQTIRGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1252..2670
                     /gene="dcm"
                     /locus_tag="ACHOQQ_RS18210"
                     /old_locus_tag="pgaptmp_003642"
     CDS             1252..2670
                     /gene="dcm"
                     /locus_tag="ACHOQQ_RS18210"
                     /old_locus_tag="pgaptmp_003642"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGU4471850.1"
                     /translation="MQENILVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2651..3121
                     /gene="vsr"
                     /locus_tag="ACHOQQ_RS18215"
                     /old_locus_tag="pgaptmp_003643"
     CDS             2651..3121
                     /gene="vsr"
                     /locus_tag="ACHOQQ_RS18215"
                     /old_locus_tag="pgaptmp_003643"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGU4471851.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDS
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3110..4030)
                     /gene="yedA"
                     /locus_tag="ACHOQQ_RS18220"
                     /old_locus_tag="pgaptmp_003644"
     CDS             complement(3110..4030)
                     /gene="yedA"
                     /locus_tag="ACHOQQ_RS18220"
                     /old_locus_tag="pgaptmp_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGU4471852.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4204..5121
                     /gene="yedI"
                     /locus_tag="ACHOQQ_RS18225"
                     /old_locus_tag="pgaptmp_003645"
     CDS             4204..5121
                     /gene="yedI"
                     /locus_tag="ACHOQQ_RS18225"
                     /old_locus_tag="pgaptmp_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGU4471853.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5200..5382
                     /gene="yodC"
                     /locus_tag="ACHOQQ_RS18230"
                     /old_locus_tag="pgaptmp_003646"
     CDS             5200..5382
                     /gene="yodC"
                     /locus_tag="ACHOQQ_RS18230"
                     /old_locus_tag="pgaptmp_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGU4471854.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSNGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPVEEV"
     gene            5625..7319
                     /gene="dgcQ"
                     /locus_tag="ACHOQQ_RS18235"
                     /old_locus_tag="pgaptmp_003647"
     CDS             5625..7319
                     /gene="dgcQ"
                     /locus_tag="ACHOQQ_RS18235"
                     /old_locus_tag="pgaptmp_003647"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGU4471855.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELAMLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7316..8131)
                     /gene="yedP"
                     /locus_tag="ACHOQQ_RS18240"
                     /old_locus_tag="pgaptmp_003648"
     CDS             complement(7316..8131)
                     /gene="yedP"
                     /locus_tag="ACHOQQ_RS18240"
                     /old_locus_tag="pgaptmp_003648"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGU4471856.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8429..8656)
                     /gene="yodD"
                     /locus_tag="ACHOQQ_RS18245"
                     /old_locus_tag="pgaptmp_003649"
     CDS             complement(8429..8656)
                     /gene="yodD"
                     /locus_tag="ACHOQQ_RS18245"
                     /old_locus_tag="pgaptmp_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGU4471857.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQHDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            8819..9007
                     /gene="dsrB"
                     /locus_tag="ACHOQQ_RS18250"
                     /old_locus_tag="pgaptmp_003650"
     CDS             8819..9007
                     /gene="dsrB"
                     /locus_tag="ACHOQQ_RS18250"
                     /old_locus_tag="pgaptmp_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGU4471858.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9051..>9123)
                     /gene="rcsA"
                     /locus_tag="ACHOQQ_RS18255"
                     /old_locus_tag="pgaptmp_003651"
     CDS             complement(9051..>9123)
                     /gene="rcsA"
                     /locus_tag="ACHOQQ_RS18255"
                     /old_locus_tag="pgaptmp_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="with RcsB is a positive regulator of capsular
                     polysaccharide synthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU4471859.1"
                     /translation="KQVIYHVVRLTDNVTNGIFVNMR"
BASE COUNT         2215 a         2221 c         2480 g         2207 t
ORIGIN      
        1 acgatttttc ctgtattcac tgccgttgcg caaaaattat ctatttgttc aaaaaatgag
       61 tgagtcttga ctggctcatc caatgtggaa aaatgtgact tttatcacat aatagtacta
      121 agtctgaatt ttccgggtta tctcaaaatg gaatacggtt cgacaaagat ggaagagaga
      181 ctctctcgca gccctggcgg caaactggct ttgtgggctt tctatacatg gtgtggctat
      241 ttcgtctggg caatggcgcg ttacatctgg gtgatgagcc ggatccccga tgcccccgtg
      301 tcgggatttg aaagcgattt aggctctacc gcgggaaagt ggttaggggc actggtcgga
      361 tttttattta tggctctggt cggggcattg ttaggcagca ttgcctggta cacccgacct
      421 cgtcccgcac gtagcagacg ctatgaataa aaattgctaa ggtggcgact ctttggcaac
      481 ggagcgcgta tggacttaca acactggcag gcacagttcg aaaactggtt gaaaaaccat
      541 catcagcacc aggacgcggc ccatgacttg tgtcattttc gccgcgtctg ggctacggca
      601 cagaagctgg cggcagatga cgatgtcgat atgttggtga ttttaaccgc ctgttatttt
      661 cacgatattg tgagcctggc gaaaaatcat ccgcaacggc agcgttcttc aattctggcg
      721 gcagaagaga cgcgtcgcct gctccgtgaa gagttcgagc aatttccggc ggagaaaatc
      781 gaggccgttt gtcatgccat tgccgctcac agtttcagcg cgcaaatcgc ccccttaaca
      841 acggaagcta aaatcgtcca ggatgcggat cggctggaag ccctgggggc gattggcctg
      901 gcgcgcgtgt ttgccgtttc aggagcattg gacgtggcgc tgtttgatgg tgaagatccg
      961 ttcgcacagc atcgcccgct tgatgataaa cgttacgcgc tggatcattt ccagactaag
     1021 ttgctgaagc taccgcatac catgcaaacc ataagaggca agcagctggc gcagcacaac
     1081 gcgcaatttt tagtcgagtt tatggcgaag ctcagtgccg aactggcggg ggagaatgaa
     1141 ggtgtcgatc acaaggtgat agatgcgttt tcacctgcgg gctgagcgcg tggccctaaa
     1201 tggctgtaat tatgttaacc tgtcggccat ctcagatggc cggttaaatc tatgcaggaa
     1261 aatatattag taaccgattc atacagcacc gggaatgccg cacaggcaat gctggagaaa
     1321 ctgctgcaaa tttatgatgt taaaacgctg gtggcgcagc ttaatggtgt gggtgagaat
     1381 cactggagcg cggcaatttt aaaacgtgcg ctggcgaatg actcggcatg gcaccgttta
     1441 agtgagaaag agttcgccca tctgcaaacg ttgttaccca aaccaccggc acatcatccg
     1501 cattatgcgt ttcgctttat cgatctattt gccggaattg gcggtatccg tcgcggtttt
     1561 gaatcgattg gcggacaatg cgtgtttacc agcgaatgga acaaacatgc ggtacgcact
     1621 tataaagcca accattattg cgatccggcg acgcatcatt ttaatgaaga tatccgcgac
     1681 atcaccctca gccataaaga aggcgtgagt gatgaggcgg cggcggaaca tattcgtcaa
     1741 cacattcctg aacacgatgt tttactggcc ggtttccctt gtcagccatt ttcgctggct
     1801 ggcgtatcga aaaagaactc gctcgggcgg gcgcacggtt ttgcctgcga tactcagggc
     1861 acgctgttct ttgatgtggt gcgcattatc gacgcgcgtc gtccggcgat gtttgtgctc
     1921 gaaaacgtca aaaacctgaa aagccacgac cagggtaaaa cgttccgcat catcatgcag
     1981 acgctggacg aactgggcta tgacgtggct gatgcagaag ataacgggcc ggatgatccg
     2041 aaaatcatcg atggtaaaca ttttctgccg cagcaccgtg aacgcatcgt gctggtgggt
     2101 tttcgtcgcg atcttaatct gaaagccgat tttactctgc gtgatatcag cgaatgtttc
     2161 cctgcacagc gagtgacgct ggcgcagctg ctggacccga tggtcgaggc gaaatatatc
     2221 ctgacgccgg tgctgtggaa gtacctctat cgttatgcga aaaaacatca ggcgcgcggt
     2281 aacggcttcg gttatggaat ggtttatccg aacaatccgc aaagcgtcac gcgtacgctg
     2341 tctgcgcgtt attacaaaga tggcgcggaa attttaatcg atcgcggctg ggatatggcc
     2401 acgggtgaga aagactttga cgatccgctg aatcagcaac atcgtccacg tcggttaacg
     2461 cctcgggaat gcgcgcgctt aatgggtttt gaagcgccgg gagaagcgaa attccgcatt
     2521 ccggtttcgg acactcaggc ctatcgccag ttcggtaact cggtggtcgt gccggtcttt
     2581 gccgcggtgg caaaactgct tgagccaaaa atcaaacagg cggtggcgtt gcgtcagcaa
     2641 gaggcacaac atggccgacg ttcacgataa ggccactcgc agcaaaaata tgcgcgcgat
     2701 tgccacgcgt gatacggcga ttgagaagcg cctcgccagt ctgttaaccg ggcagggcct
     2761 ggcgtttcgc gttcaggact ccagtctgcc cggacgcccg gattttgtcg ttgatgaata
     2821 tcgctgcgtg atatttaccc atggctgctt ctggcatcat catcactgct atctgtttaa
     2881 agtgcctgcg actcgaaccg agttctggct ggagaagata ggtaaaaatg ttgagcgcga
     2941 tcgccgcgat atcagtcgct tgcaggaact cggctggcgc gtattgattg tctgggagtg
     3001 cgcgttacgt gggcgcgaga agctgacgga tgaagcgctt agcgagcgtc tggaagagtg
     3061 gatctgcggc gaaggtgcca gcgcgcagat cgacacgcag gggatacatt tactcgcttg
     3121 atgcgtcctg aataactggc gcaactacgg gttttgccgg aaagagatat tttcccaacg
     3181 tgaccagtac caccgcgaag acaattacgc cgagcgccag ccattcaatc ttcgacagtg
     3241 tttctccacc cagtcccgta cccagcaaga ccgcgaccac cgggttaacg taggcgtagc
     3301 tggtggcgag agccggactg acgttgcgga ttaaatacat ataagcgtta atggcgataa
     3361 tcgaaccaaa cagcgccaga tagccgaccg caaggaagcc tgaaagggaa gggagcgccg
     3421 tcagtttttc acccgcaatc atcgacgcga tcattaacac cacgcctgcc gccagcatct
     3481 caatcgcacc cgccatcatc cctacaggta aggtaatgcg cgagccataa actgagccaa
     3541 acgcccagct aatcgagccg attaaaatca gaatcgcgcc ccacggattg ccgcttaaat
     3601 ttccaccgct attgagcatg atgattccgg caagcccaat ggcaataccc acccattcca
     3661 gtttgcgcgt tttaatgcca aacaggcggc tgaagcacag ggtaaagagg ggcacggttg
     3721 caaccactac ggcggcgatg ccggaaggaa cattttgatg ttcggcaacc gtcaccatgc
     3781 cattaccgac agccagcaat aacaggccaa tcagcgcggc attgagcagc ggacgtagcg
     3841 gggggagctt gtgtccgcgc agtagcaaaa atgccagcaa taaaataccg gctgccagga
     3901 atcgaacgcc cgccatcatt aatggaggcc agctttccac gccaatccga atgacaaaat
     3961 aggttgagcc ccaaatgata tacaacgcaa acagcgcgcc aaaaagcggt aataactggc
     4021 ggaaacgcat aatccctcac ggtggaaata aaaaggtggt tcatagtaaa cgtgaaaatc
     4081 attctgctgg cgagagatat aattgcactt gattgttaaa aaaaatgttg acctgtgaag
     4141 caagtatcag agctgccgtt tttgcttcat acttacaccc ttcaacaata aaaatgagag
     4201 ggaatgcttt tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg
     4261 gacgatatct ccgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat
     4321 gacttatcgc tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg
     4381 gtctggggcg tggcgaaagg atcgctgatc aataaagtga ttctggtgcc actggcgctg
     4441 atcatcagtg cgtttatccc gtgggcgatt acgcctctgt tgatgattgg tggcgcgttt
     4501 ctctgctttg aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa
     4561 gatccggcgc agagtcagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt
     4621 gaaaaggaca aaataaaagg ggcgattcgt accgatttta tattgtctgc cgaaatcgtc
     4681 gccatcacgc tggggattgt ggccgaagcg ccgttgctta atcaggtgct ggtgctttca
     4741 ggcatcgcgc tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt
     4801 gatgacctgg ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa
     4861 ggattattga ttatcgcgcc ctggctgatg aaagcgttat cgattgtcgg cacgctggcg
     4921 atgttcctcg tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgccatt
     4981 gaacatttcg ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat
     5041 ctgattcttg gatttatcat cggcggcatc gtggtgctgg gagtgaaagc cgtagcgaaa
     5101 atgcgcggtc aggcacatta atactcgcac aggctattat gcaaaattcg ccatcttcaa
     5161 ctaaggtgaa caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag
     5221 gaagttacgg ttaaagaggg cggcccgcgg atgattgtca ctggatactc caacggtatg
     5281 gttgagtgtc gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgag
     5341 cttgttccgg gggaggggag tcgtcctgtg gaagaagttt gaaagggaat cattaacacc
     5401 aggccggatg tggcgctcac gtcgcatccg gtcgggtgtg ccagtgtctg cactgaattg
     5461 cctgatgcga cgctatcgcg tcttatcatg cctacgttat aaaacaaaac tatcgatgtc
     5521 tgtcgcgtct tatcatgcca ccattataaa attatcgctg ttttgcggta cgctatcgcc
     5581 aggttatgcc agaatcataa aaaagcaggt tgggagtcgt cagggtgcag cacgagacaa
     5641 aaatggaaaa ccagagctgg ttgaaaaaac tcgcacgccg cctggggcct ggtcatgtcg
     5701 ttaatctctg ctttatcgtg gtattgcttt tttccacctt gctcacctgg cgtgaagtgg
     5761 tagtgctgga agatgcctat atctccagcc agcgtaatca tctggaaaac gtagccaacg
     5821 cgctcgataa gcatttgcag tataacgtcg acaaactgat ctttttgcgt aatggcatgc
     5881 gcgaagctct cgtagcgcca ctggatttca cctctctgcg tgatgctgta accgagttcg
     5941 aacagcatcg cgacgagcac gcctggaaaa tcgaactcaa ccgacggcgc accctgccag
     6001 tcaatggcgt gtcggatgca ttagtcagcg aggggaatct cctgtctcgc gaaaatgaaa
     6061 gcctcgacaa tgaaattacc gctgcactgg aagttggtta cttgctgcga ctagcgcaca
     6121 actcctcgtc gatggttgaa caggcgatgt atgtctcgcg tgccggattt tacgtttcga
     6181 cgcagccgac cttgtttacg cgcaatgtcc cgacgcgtta ttacggctat gtcacccaac
     6241 cctggtttat cggccattca caacgagaaa atcgtcaccg cgcggtacgc tggttcactt
     6301 cgcaaccgga acacgccagc aatactgaac cgcaggttac cgtcagtgtt ccggtagaca
     6361 gtaataacta ctggtatggc gtgctgggga tgagtattcc cgtgcgtact atgcagcaat
     6421 ttttaagaaa cgccatcgat aaaaacctcg atggtgagta tcagctctat gacagtaagc
     6481 tgagattttt gacctcttcc aatcctgacc atccaacagg gaatattttt gatcctcgtg
     6541 aactggccat gctggcgcag gcaatggaac atgacacgcg gggcggcatt cgtatggaca
     6601 gtcgctatgt tagctgggaa cgtctggacc atttcgacgg tgtgctggtg cgcgtccata
     6661 cgttaagcga aggcgtgcgc ggcgatttcg gcagtatcag cattgcatta accctgctgt
     6721 gggcgctctt taccaccatg ttactcatct cctggtatgt gattcgccgg atggtcagca
     6781 acatgtatgt tctgcaaagc tcgttgcagt ggcaggcgtg gcacgacacc ttaacgcgtt
     6841 tatataaccg tggcgcactg ttcgaaaaag cccgtccgct cgcgaaattg tgtcagacgc
     6901 accaacatcc tttttctgtc attcaggtcg atcttgacca ttttaaagcg attaatgacc
     6961 gctttggtca tcaggcgggc gaccgtgttc tttctcatgc tgccggatta attagcagtt
     7021 ccttgcgtgc gcaggacgtt gccgggcggg tcggtggtga ggagttttgt gtgattctgc
     7081 caggcgcgag tctgacggag gctgcggaag tcgcagaacg tattcgcctg aagttaaatg
     7141 aaaaagagat gttgattgct aagagtacga cgatacgcat cagtgcctcg ttgggggtaa
     7201 gtagcagcga ggaaaccggt gattatgatt ttgaacaact ccagtcactg gccgaccgtc
     7261 ggctttatct cgctaaacag gctgggcgta atcgggtatt cgcgagcgat aacgcttaac
     7321 gggcggagaa aaaatggtcc agcccttcac gccatccttc cggtccttca cgctgcgttc
     7381 gccagacgcg ggccggatcc tcatcatgca gatgcacccc ttcacggttt agccctttca
     7441 caatcaccgc gtaatccatt acctccagta agggcgcatc gtttggccca tcgcccaggc
     7501 caagtgtggt tgggcgtttg cctgacaatt gttgataggt cgcgataatc cagttggcag
     7561 cctgatcttt tccggcagag gcatccagta cgtgccagaa gcgcgcacct tgcataaact
     7621 gtaagcccag ttcgttcaga cgagcggtaa attgtgccat acgctcgtca ctgtcgcgcc
     7681 aaattagcgt taccgacgcc tcatgaagct gcgtcagcgc cgcctggcta cggcttaatc
     7741 ccgtccattc ggcgatggtt gcatcgtcga catcatcaaa agtcgtgaat ttaaaatgtt
     7801 ctttctcgcg tagcgtattt aaaacctggc tgatttcgcc atggctaata cctgagatga
     7861 tgcgtggaaa accgtctatc tcctgccatt gctcagcaag ctggatcact gcgccatttt
     7921 cggcaatcag cggtaaacct tgtagcccca acgttttttg caagtacagc atttccgctg
     7981 atgttttact gctacagaga atgacgggaa catttgcttc gcgtaaacgg gtgagccagg
     8041 gggctgccgg ttgccagtca taactatgac tgtccagcag ggtgccatca agatcgctaa
     8101 aaaccagtag tggttgttga attgaaaaca tagttgcgca gtactcccct taccaggatg
     8161 attctcaaaa cagcacaaat tatcaaagat gattttttcg ggagcgatcc cgctgacgcc
     8221 agagccacca aggggtgacg tgcgtcacat ttctattcat aagtagcgtt aatcattcat
     8281 atggcgaata ttttcttgtc agcgaaaaaa attgcggata aggtgatgaa cacatcagat
     8341 ttcctggtgt aacgaatttt ttaagtgctt cttgcttaag caagtttcat cccgaccccc
     8401 tcagggtcgg gattttttta ttgtgcattc aacgattcac ttcagagacg ctgaagtcat
     8461 gaatatccag ttcgaaggca tccgccaatt cacgccatgt atgatattca cgtccatcga
     8521 cgctaacgtg ttcagaatcg tgctggctgc gatgaacttc gctttcgcta atttcattga
     8581 tcgccgccag cagggcatcg acatcgacgc tgacctcacg ttttgcggta tcgctgtact
     8641 cttttgcggt tttcatcatc tttcctcgca accgttactc tgttgttgcc gccagtcgtt
     8701 aagtccgcct ggcatctctt aagtatagac cgtcgagaaa atcagcactc gcgcgcgcct
     8761 ggcgctgcca gaaaaacgaa attgttctac actggcacaa agccacagga ggaaaacgat
     8821 gaaggtgaat gatcgggtaa cagtcaaaac ggatggcggt ccgcgtcgtc ctggcgtggt
     8881 actggcagtt gaggagttta gtgaaggcac aatgtacctg gtttcgctgg aagactaccc
     8941 gctcggcatc tggttcttta atgaagcagg gcatcaggac ggtatctttg tggagaaagc
     9001 agagtaatct tattcagcct gactggtggg aaaccaccag tcagaatgtg ttagcgcatg
     9061 ttgacaaaaa taccattagt cacattatcc gtcagtcgga cgacatggta gataacctgt
     9121 tta
//
LOCUS       DAZKFU020000192         8940 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZKFU020000192 DAZKFU020000000
VERSION     DAZKFU020000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.194"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(219..1271)
                     /gene="fbaB"
                     /locus_tag="ACHOQQ_RS18260"
                     /old_locus_tag="pgaptmp_003652"
     CDS             complement(219..1271)
                     /gene="fbaB"
                     /locus_tag="ACHOQQ_RS18260"
                     /old_locus_tag="pgaptmp_003652"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGU4471860.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1528..2805
                     /gene="yegT"
                     /locus_tag="ACHOQQ_RS18265"
                     /old_locus_tag="pgaptmp_003653"
     CDS             1528..2805
                     /gene="yegT"
                     /locus_tag="ACHOQQ_RS18265"
                     /old_locus_tag="pgaptmp_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HGU4471861.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLITAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2802..3806
                     /gene="yegU"
                     /locus_tag="ACHOQQ_RS18270"
                     /old_locus_tag="pgaptmp_003654"
     CDS             2802..3806
                     /gene="yegU"
                     /locus_tag="ACHOQQ_RS18270"
                     /old_locus_tag="pgaptmp_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGU4471862.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRGEFTDDTSMALCLADALLEREGNIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREVV
                     "
     gene            3803..4768
                     /gene="yegV"
                     /locus_tag="ACHOQQ_RS18275"
                     /old_locus_tag="pgaptmp_003655"
     CDS             3803..4768
                     /gene="yegV"
                     /locus_tag="ACHOQQ_RS18275"
                     /old_locus_tag="pgaptmp_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU4471863.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4742..5488)
                     /gene="yegW"
                     /locus_tag="ACHOQQ_RS18280"
                     /old_locus_tag="pgaptmp_003656"
     CDS             complement(4742..5488)
                     /gene="yegW"
                     /locus_tag="ACHOQQ_RS18280"
                     /old_locus_tag="pgaptmp_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4471864.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5540..6358)
                     /gene="yegX"
                     /locus_tag="ACHOQQ_RS18285"
                     /old_locus_tag="pgaptmp_003657"
     CDS             complement(5540..6358)
                     /gene="yegX"
                     /locus_tag="ACHOQQ_RS18285"
                     /old_locus_tag="pgaptmp_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGU4471865.1"
                     /translation="MQLRITSRKKLTALLCALVLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPGQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKLIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(6423..7223)
                     /gene="thiD"
                     /locus_tag="ACHOQQ_RS18290"
                     /old_locus_tag="pgaptmp_003658"
     CDS             complement(6423..7223)
                     /gene="thiD"
                     /locus_tag="ACHOQQ_RS18290"
                     /old_locus_tag="pgaptmp_003658"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGU4471866.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7220..8008)
                     /gene="thiM"
                     /locus_tag="ACHOQQ_RS18295"
                     /old_locus_tag="pgaptmp_003659"
     CDS             complement(7220..8008)
                     /gene="thiM"
                     /locus_tag="ACHOQQ_RS18295"
                     /old_locus_tag="pgaptmp_003659"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGU4471867.1"
                     /translation="MQVDLLSFAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAI
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVATGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8043..8139)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8581..8694
                     /locus_tag="ACHOQQ_RS18300"
                     /old_locus_tag="pgaptmp_003660"
                     /pseudo
     CDS             8581..8694
                     /locus_tag="ACHOQQ_RS18300"
                     /old_locus_tag="pgaptmp_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012539895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2090 a         2294 c         2299 g         2257 t
ORIGIN      
        1 acgacaatat gaaaattaat agaggaatga aaggcaaaaa aaaacggcct cccgtaggga
       61 agccgcaaca aagtgcgcgt ggttttatgc aggatgcggc gtaaacatat gtggcagcgc
      121 tgcgtaggca tgataagacg cttcagcgtc gcatcaggca taggttgccg gatgcggcgt
      181 aaacgcctta tccgtcctac ggggtagtgc cgttcgtatc aggcgatagt aattttgcta
      241 tcgagataaa cgtcctgcac ggcgttaatc agtttcacgc cgtcagccat cgatttcttg
      301 aacgccttac gtccaagaat cagccccatt ccgccagcgc gtttgttgat aaccgcagta
      361 cgcactgcat cactgaggtc agtttcaccg cctgcagcac cgccggagtt tatcaacccg
      421 gcccggccca tatagcagtt agctaactga taacgcacca gatcaatcgg gttttcgctg
      481 gttaacttgc tgtacacgcg atcgtcggta taaccgtaat taattgcttt atagccgccg
      541 ttattttccg ccattttttg tttgacgata tctgcaccta tggttgccgc cagatggttc
      601 gcctgaccag tcaggtcggc ggagacatgg taatcaacgc catctttctt aaaggcggag
      661 ttacgcaaat aggcccacag cactgtcacc atgcccagct cgtgcgcacg ttcaaaagcc
      721 gcagaaattt cttcaatctg gcgacgtgac tcttccgagc caaaatagat agtcgcacca
      781 accgccaccg cacccatgtt gaacgcctgc tccacgctgg catacagcgt ttgatcgtag
      841 gtattcgggt aacttagcgt ctcgttgtga ttaagtttga cgagaaatgg aatgcgatgc
      901 gcgtaacgcc gcgataccga cgccaacacg ccgtaagttg acgccacaca gttacagccc
      961 gcttcgatcg ccagttcaac aatgtttttc gggtcaaagt agagcgggtt agcggcaaat
     1021 gaagctccag cagagtgctc aacgccctgg tcaacgggca gaatagaaag atatcctgtg
     1081 ccagccagac gcccggtgtt gtacaacgtc tgcatattac gtaacaccgc tggcgggcga
     1141 ttattgtcaa tcatcacgcg gtctacgtag tcatgtccgg ggagataaag ctggtcagaa
     1201 ggaatagtca tacaacggtg ctgtaaaagg ttgtcggcgt ctttgccaag caactgcgca
     1261 atatctgtca ttactatgct cccgtaaatt ccgattggat atcggctatg gattgtcctg
     1321 gcccgccttt tgcgggcaat cataatcctg gtcgttacgg gcgagttttt ccatcttttg
     1381 gatgtttttt cagcgttttc tgttggctcg attcatcaga aaaaatgtta gcgcggtcaa
     1441 gttttcaccg caaaggtatt taaaaggtat tattaagtgg tattgtcatc gcgtacctta
     1501 cattacctgt catgaaggaa ttaaaagatg aaaacaacag caaagctgtc gttcatgatg
     1561 tttgttgaat ggtttatctg gggcgcgtgg tttgtgccat tgtggttgtg gttaagtaaa
     1621 agcggtttta gtgccggaga aattggctgg tcgtatgcct gcacagccat tgcggcgatc
     1681 ctgtcgccaa ttctggttgg ctccatcact gaccgctttt tctcggcgca gaaagtgctg
     1741 gcggtattga tgttcgctgg tgcggtgctg atgtatttcg ccgcgcaaca gaccactttt
     1801 gccgggttct tcccgttact gctggcctac tcgctaacct atatgccgac cattgcgctg
     1861 actaacagca tcgcttttgc caacgtgccg gatgttgagc gtgatttccc gcgcattcgt
     1921 gtgatgggca ctatcggctg gattgcctcc ggtctggcat gtggtttctt gccgcaaata
     1981 ctggggtatg ccgatatctc accgactaac atcccgctgc tgattaccgc cggaagttct
     2041 gctctgctcg gtgtgtttgc gtttttcctg cccgacacgc caccaaaaag caccggcaaa
     2101 atggacatta aagtcatgct cggcctggat gcgctgatcc tgctgcgcga taaaaacttc
     2161 ctcgtctttt tcttctgttc attcctgttt gcgatgccac tggcgttcta ttacatcttt
     2221 gccaacggtt atctgaccga agttggcatg aaaaacgcca ccggctggat gacgctcggc
     2281 cagttctctg aaatcttctt tatgctggca ttgccgtttt tcactaaacg ctttggtatc
     2341 aaaaaggtat tattgcttgg tctgatcacc gctgcgatcc gctatggctt ctttatttac
     2401 ggtagtgcgg atgaatattt cacctacgcg ttactgttcc tcggtatttt gcttcacggc
     2461 gtaagttacg atttttacta cgttaccgct tacatctatg tcgataaaaa agcccccgtg
     2521 catatgcgta ccgccgcgca ggggctgatc acgctctgct gccagggctt cggcagtttg
     2581 ctcggctatc gtcttggcgg tgtgatgatg gaaaagatgt tcgcttatca ggaaccggta
     2641 aacggactga ctttcaactg gtccgggatg tggacttttg gcgcggtgat gattgccatt
     2701 atcgccgtgc tgttcatgat ttttttccgc gaatccgaca acgaaattac ggctatcaag
     2761 gtcgatgatc gcgatattgc gttgacacaa ggggaagtta aatgaaaaca gaacgtattc
     2821 tcggtgctct ttatgggcag gcgttagggg atgcgatggg gatgccctcc gagctttggc
     2881 cacgcagccg cgttaaagca cactttggct ggattgaccg ttttctgcct ggaccaaagg
     2941 agaataacgc ggcctgttat tttaaccgcg gcgaatttac cgatgacacc tcgatggcgc
     3001 tgtgtctggc ggatgcgtta ctggaacgtg aaggcaatat cgacccgaat ctgattgggc
     3061 gtaatattct cgactgggcg ctgcgtttcg acgcctttaa caaaaacgta ctaggtccga
     3121 cctcgaagat tgctctcaac gccattcgcg acggtaaacc cgttgctgaa ctggaaaaca
     3181 acggcgtgac caacggcgca gcgatgcgcg tctcgccatt aggttgtttg cttccggcgc
     3241 acgatgttga ttcctttatt gatgatgtgg cgctggcgtc cagcccgacc cataaatccg
     3301 atctggcagt tgcaggtgcg gtagtcatcg catgggcgat ttctcgtgcc attgacggag
     3361 aaagctggtc agcgattgta gattcactgc cttcaattgc gcgacatgca cagcaaaaac
     3421 gcatcactac cttcagcgcc tcactggcag cacgtctgga gattgcgctg aaaattgtgc
     3481 gcaatgccga cggcactgaa tccgccagcg aacagcttta ccaggtcgtt ggcgcaggta
     3541 ccagcactat tgagtccgtt ccgtgcgcca ttgcgctggt tgaactggca caaaccgacc
     3601 cgaaccgttg cgccgtcctg tgcgctaacc ttggcggcga cacagacacc atcggtgcta
     3661 tggcgacggc aatctgcggc gcgttgcatg gcgttaacgc tatcgatcct gcattaaagg
     3721 cggagctgga tgcggtaaat cagcttgatt tcaaccgcta tgccacagcg ctggcgaaat
     3781 atcgtcaaca acgggaggtg gtatgagcgg cgctcgatta cacacgctgc tgcctgaatt
     3841 aaccacgcgt cagccggtga tggtcgtcgg cgcggcggtc attgacgtga tcgccgacgc
     3901 ctacgccctc ccctggcgtg ggtgcgatat cgaactgaaa cagcagagcg ttaacgttgg
     3961 cggctgcgca ctgaatattg ccgtggcgtt aaagcgcctc ggcatcgaag cgggtaatgc
     4021 cttgccgctc ggtcagggcg tgtgggcgga gattattcgc aaccggatgg cgaaagaggg
     4081 gttaatcagc ctgatcgata acgccgaagg cgataacggc tggtgtctgg cgctggttga
     4141 gccggatggc gaacgtactt ttatgtcatt cagcggtgtg gaaaatcagt ggaatcgcca
     4201 gtggctggcg cgattaaccg ttgcgcctgg cagcctgctc tatttttccg gttatcaact
     4261 ggcctcgccc tgcggcgaat tgttagtgga atggctggaa gagctgcaag acgtgacgcc
     4321 gtttatcgat tttggcccac gtattggcga tatcccggat gcattactgg cgcggatcat
     4381 ggcctgtcgc cctttagtgt cgctcaatcg tcaagaggct gagattgccg ccgaacgttt
     4441 tgctttatcc gcagagataa caacacttgg caaacaatgg caggagaaat ttgccgcgcc
     4501 gttgatcgtt cgtctcgata aagaaggcgc atggtatttc agcaacgacg cttctggctg
     4561 cattccggca tttccaacgc aagtcgtcga caccattggg gcgggcgaca gtcatgccgg
     4621 tggcgtactt gccgggctgg cctctggtct gccactggcg gatgccgtat tactgggcaa
     4681 tgcagtggcg tcgtgggttg tcgggcatcg gggcggtgat tgtgcgccaa cgcgcgagga
     4741 actactcctc gcacacaaaa acgtatagat cgctgcgaca gtggctaatg ctgtactcaa
     4801 taggccgttg ctgttggtca agcgccactt gcttgatcac cagcaccggt attttgctat
     4861 ctaactgaat atgtgactga aactcggcat ctggcatccg ggcgctaacg cgggaacgtg
     4921 tacgttgtgg gtaaatgtgc tggctgcgga aatagtcata aagagaaatt ccgatggcat
     4981 caacatcatg aattaaatgc gccggaaccc acgattcctc aatcgatacc gcttcttcat
     5041 cgacatagcg aatacgctta agcaaaaaga catcacttcc cgcttcgacc gccagctgct
     5101 gcgcgacttc ttcgggacat ttcacgacac gcttgttaac ccatagcgta tcgggctttt
     5161 tcccgcgcaa taccacctgc tgagaaaaac ctcgcgcttc tttcaacgag tattcgaaga
     5221 tgttgttgat ctgcgtgccg taaccgcgcg aacgcgtcac cacgccctct tcttccagcg
     5281 cctgcatcgc cttgcgcacc gtaatgcgtg aaacgccggt taactgactt aaatcacgct
     5341 caccgggcaa aatattgcca tgctccagca ccccgctgcg cacggcattt tttaccgttt
     5401 cggcaaactt aatataaagc ggcgtgttat ccgccgctaa aatacgctcg ttaagttggg
     5461 caatcagctg ggtatgcgct tgttccattt gtctttttcc tgccagggag tctggtggga
     5521 gtatatcact gcttcttatt taaggcgttt ctttaatccc atcaacgaat gcctgtaact
     5581 cctccaccgt gccattaaac acattaaaat ccaccggacc attgatgcca tctacctgtc
     5641 cacggtcgga atgctgccag aagcgccagg ccatgccgtc attgtccgga cgacgttgat
     5701 agtagtgagc cacccaccac ggatattcat tgaaatagcc cgccagattg gtgtgataaa
     5761 aaacggctcc tgagtaaata atcagctttt ttcccgtact tttttcgacc atttttagcc
     5821 actgacttac ccgcttgcgt aattcttttg ccgataattt tccccgttct tctacgtcca
     5881 gcacggcagg gaaatcgcct tgtgagaaat ccaccgtttg cagaaataat cgcgcctgac
     5941 caggcgctgc taccgacggg gagaaataat gatacgcccc gcgcagcagg ccattttcgc
     6001 ggcttagttg ccagttacgc gaaaaatagg ggtccaccag cttttcgcct tccgtagcct
     6061 taataaaagc aaactgtaag cggataccgt tgtcgcgcat ttttgccacc cgctgccagt
     6121 cgatccgctc ctgccagcgc gaaacatcta ttccgtgaat ggtgtaactg gcaggaatgc
     6181 gaatagcgaa agatttaacc gggcgataac cgtagaagtg gatgtagtct gtaatctgaa
     6241 ctgctgtcga atagaaaaaa ttcaccgtct gacgcggata aattgcaacg atggaaatta
     6301 gcacaagagc gcataataga gcggtgagtt tttttcgact ggtgattctc agctgcattg
     6361 gtttcatccc tgaatgtcag tgccaaaggc tgacaataac caaagcagct atagtacggt
     6421 gctcaccacc aggcgtggaa gtggtgaacc ggaccaatac cgtgaccaac ttccagcgtg
     6481 tcggcctggg ctaatgctga tgaaagccag ctttttgcct cctgtacggt gtcagcccag
     6541 tttgtatggc gcgggcgtag tgcagccaac gccgcagaga gcgtacaacc agtgccgtgg
     6601 gtgtttttgg tcataatgcg cggtgcggta aaccgttgtt caccctcgcg ggtaaacagc
     6661 cagtccgggc tttgctcatc atccagatga ccacctttca ttagcactgc gccacagccc
     6721 atcgccaaca gcgatcgccc ttgttccagc atttcctgtt cggtgcgcgc gtgtggcgcg
     6781 tcgagcaagg cagcagcttc gggcaagttt ggcgttatta atgaaacctg tggcaataat
     6841 cgactgcgca acgtagcaac cgccgaaggt gaaagcagcg ggtcgccgct ttttgccagc
     6901 ataacggtgt cgagtaccac gttttggatc tgataacgtt gcaaccgttc tgccaccgct
     6961 tcaacaatat cggtttccgc caacataccg attttagtgg tatcgattcg cacatcgctg
     7021 aacaccgaat cgagctgggc ggcgacaaaa tcaggctcaa tgcgatacac cgactgtacg
     7081 ccacgggtat tttgcgccac cagtgcagta ataactgagc aaccataagc gccaagtgcc
     7141 gagaaggttt taagatcggc ctgaatcccc gcaccaccac tcggatcagt accggcaatc
     7201 gtcagagcgt taattcgttt catgcctgca cctcctgcgt caattgccag agcgcatcaa
     7261 ggaaatgtgg aacaaaactg cctggcccct cgcttctggc gactgcgcgt tctccggctt
     7321 gtttcatcca gtgacaggca gatgcgacat tttccagcgt atcgcctggt aacgcacagc
     7381 aggcagcgac aaccgccgat aacgcacagc cagttcctac cactttggtc attaacggat
     7441 cgccaccgtg aataccaatg atacgatgtc catcggtaac ataatccacc tcgccagtga
     7501 ccacgacgat tgcgccagtt tcccgtgcca gtgtttgtgc agcgggtatc gcattagctg
     7561 ccgcgtcagt ggtatccact ccccgtccgc cagtagcaac gccagctaat gccatgattt
     7621 ccgaagcatt accacgtatc gctgccggtt taaaagataa aagttcatga caaaaatggc
     7681 gacgataatc gagcgcacca accgctactg gatcaagcgt ccagggggtt tgagagcttt
     7741 ttgcttgctc aacggcggca cgcatcgcct gtgcgcgtgg ctgcgtcagc gtgccaacgt
     7801 taatcaacaa ggcactggcg atagccgcaa actgactggc ctcttcggtt tcgataacca
     7861 tcgctggcga tgcaccgatc gccagcaagg tattggcagt aaaggtttgc accacatcgt
     7921 tggtcatgca gtgcacaaga ggggaatgtt ggtgaaaaag gtgtaacgtg tgcgcagatt
     7981 gcgcgaaact cagcaggtcg acttgcatag tttgctcctg ccataacgtg aagaagcaat
     8041 gacctggtgg tccgtgactt ccctacgctg gcattatcca gatcaggtga tacgggtatt
     8101 tctcagcctt cacgcagaag ggcaccccga gtcgtttggt tgaatctcta tattttgtgc
     8161 tagattaggg gaagtggctt gcagatgcaa atttaccctc aagtcatttt cccggcttac
     8221 gtggtagctg gcgtagccag cagagaatcg ggtcatataa atatgaccag cctgtatcat
     8281 acaacctttt gtaagaaggc ttcagtatta tcacgccgga ccaagtatgg atagaaaaga
     8341 tcacatacct ttataccata aatgcagttt gtatctgaca atagtgatta acctgtagtg
     8401 atattccatt attggttggt cattttttgc agattactgc atcattagtc tgtaacagac
     8461 tacagataga gaggttggag cgtaagcttc tataaaacgt tgttaagtac ttcgtataat
     8521 cgtgcaattc gcatttagag atcctcaggc ataatcaatc tgccaatgaa ggggagcgcc
     8581 atgcataaag cccgtattac tgaacatcac gccgtgatta tatcgattga atccggacaa
     8641 accgttaaag atgtctcccg ggatgccggt atctctgaag ccatctacta caactgaaag
     8701 tccagattag gcggcatgag ggcttcttat attaaaaaga aaatgactct tgatgcacat
     8761 gggtaatggt ccggaatgta tctttctgag gctgtctgaa cgggactgag aaacatggtt
     8821 taatactgga gtttatcttg ccggataaac cgacaaagaa cgctttcatg gagcgtttta
     8881 ataagacata cttgtacata aatactcgat ttttatctat tcagaacgct gaaagaaatg
//
LOCUS       DAZKFU020000193         8897 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZKFU020000193 DAZKFU020000000
VERSION     DAZKFU020000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.195"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1376)
                     /gene="trkA"
                     /locus_tag="ACHOQQ_RS18305"
                     /old_locus_tag="pgaptmp_003661"
     CDS             complement(<1..1376)
                     /gene="trkA"
                     /locus_tag="ACHOQQ_RS18305"
                     /old_locus_tag="pgaptmp_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGU4471868.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1398..2687)
                     /gene="rsmB"
                     /locus_tag="ACHOQQ_RS18310"
                     /old_locus_tag="pgaptmp_003662"
     CDS             complement(1398..2687)
                     /gene="rsmB"
                     /locus_tag="ACHOQQ_RS18310"
                     /old_locus_tag="pgaptmp_003662"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGU4471869.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDNWLALLDEAGMKGFPHTDYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(2733..3680)
                     /gene="fmt"
                     /locus_tag="ACHOQQ_RS18315"
                     /old_locus_tag="pgaptmp_003663"
     CDS             complement(2733..3680)
                     /gene="fmt"
                     /locus_tag="ACHOQQ_RS18315"
                     /old_locus_tag="pgaptmp_003663"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGU4471870.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(3695..4204)
                     /gene="def"
                     /locus_tag="ACHOQQ_RS18320"
                     /old_locus_tag="pgaptmp_003664"
     CDS             complement(3695..4204)
                     /gene="def"
                     /locus_tag="ACHOQQ_RS18320"
                     /old_locus_tag="pgaptmp_003664"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGU4471871.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4334..5458
                     /gene="dprA"
                     /locus_tag="ACHOQQ_RS18325"
                     /old_locus_tag="pgaptmp_003665"
     CDS             4334..5458
                     /gene="dprA"
                     /locus_tag="ACHOQQ_RS18325"
                     /old_locus_tag="pgaptmp_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGU4471872.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5430..5903
                     /gene="smg"
                     /locus_tag="ACHOQQ_RS18330"
                     /old_locus_tag="pgaptmp_003666"
     CDS             5430..5903
                     /gene="smg"
                     /locus_tag="ACHOQQ_RS18330"
                     /old_locus_tag="pgaptmp_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGU4471873.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5932..6474
                     /locus_tag="ACHOQQ_RS18335"
                     /old_locus_tag="pgaptmp_003667"
     CDS             5932..6474
                     /locus_tag="ACHOQQ_RS18335"
                     /old_locus_tag="pgaptmp_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGU4471874.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPICGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6479..7051
                     /gene="tsaC"
                     /locus_tag="ACHOQQ_RS18340"
                     /old_locus_tag="pgaptmp_003668"
     CDS             6479..7051
                     /gene="tsaC"
                     /locus_tag="ACHOQQ_RS18340"
                     /old_locus_tag="pgaptmp_003668"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGU4471875.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVAALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7056..7874
                     /gene="aroE"
                     /locus_tag="ACHOQQ_RS18345"
                     /old_locus_tag="pgaptmp_003669"
     CDS             7056..7874
                     /gene="aroE"
                     /locus_tag="ACHOQQ_RS18345"
                     /old_locus_tag="pgaptmp_003669"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGU4471876.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLMRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQTLGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7871..8128
                     /locus_tag="ACHOQQ_RS18350"
                     /old_locus_tag="pgaptmp_003670"
     CDS             7871..8128
                     /locus_tag="ACHOQQ_RS18350"
                     /old_locus_tag="pgaptmp_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGU4471877.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8104..8658)
                     /locus_tag="ACHOQQ_RS18355"
                     /old_locus_tag="pgaptmp_003671"
     CDS             complement(8104..8658)
                     /locus_tag="ACHOQQ_RS18355"
                     /old_locus_tag="pgaptmp_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGU4471878.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2213 a         2167 c         2228 g         2289 t
ORIGIN      
        1 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
       61 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
      121 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
      181 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
      241 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
      301 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
      361 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
      421 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
      481 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
      541 acgatcgtat tctgtaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
      601 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
      661 ccaaccagca tgatccgctt atacggtttt tctagtcgct gtaattcact catcaccgcg
      721 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcttcaac aatggtcgaa
      781 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
      841 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
      901 ttaaccacag ccaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
      961 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
     1021 atcggtacag catctgaatg aaatagctta tcggcatcgc gcacgtagtc tggtgagcgg
     1081 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
     1141 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
     1201 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
     1261 aaggtccgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
     1321 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
     1381 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
     1441 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
     1501 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
     1561 gaacaggtgg catagaccag agttccaccg gattttaaat gcggccaaat ggcgtcgaga
     1621 atttcagact gcaattgcgc gagttccggg atatcgcgat cgcgacgtaa ccatttaata
     1681 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
     1741 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
     1801 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
     1861 accgcaacaa cctgcgcttc tggtgccacc tcaaggatat gcgttgtttt accgccgggg
     1921 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
     1981 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
     2041 ggtgcaggtg tttccagccg tacagcatca gggtaatccg tatgtgggaa gcctttcatt
     2101 cctgcttcat ccagcaatgc aagccagttg tcgcgggaat gatgcgtacg attaatgcgc
     2161 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
     2221 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgagcatc actggcatta
     2281 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gtttatcaac
     2341 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
     2401 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
     2461 gtccgctgtt tgccggtcat cggacgggcc attaacttat taatcagcca gtctaactgc
     2521 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
     2581 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
     2641 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
     2701 cccggcaaga ccgggcttgg aagagtggac tatcaggcca gacggttgcc cggaacaaac
     2761 cattcccgac gagaattcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
     2821 aatgagagca ggttcaggat tccatcacca gtcgcaacct gaatgccttg tttgttggct
     2881 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
     2941 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
     3001 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
     3061 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
     3121 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
     3181 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
     3241 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
     3301 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
     3361 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
     3421 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
     3481 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
     3541 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
     3601 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
     3661 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
     3721 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
     3781 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgctt
     3841 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
     3901 gcactaaagc acgttgttca gggatcgaca ggcatccttc ttcaatgcct gtttcgccgc
     3961 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
     4021 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
     4081 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
     4141 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
     4201 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
     4261 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
     4321 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagca tcagcagctt
     4381 gtacggcgat gatatggtcc gtatcgctca ctgggtggca aaacagtcgc atattgatgc
     4441 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
     4501 acgaaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
     4561 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggcgcact
     4621 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
     4681 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagcg
     4741 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtggcgc ataaagcggc
     4801 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
     4861 gcgtcgccat gcccgactgg ctaccagttt gcttgaacat ggtggggcac ttgtctcgga
     4921 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
     4981 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgcagtggtt cgctggtgac
     5041 agcacgttgt gcgcttgagc aggggcgtga agtttttgcc ttgccaggtc cattagggaa
     5101 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
     5161 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
     5221 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
     5281 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
     5341 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
     5401 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
     5461 cctatattca cactgaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
     5521 ccgacgcagg ttttgagcga gaagatatct acaatgccct gctatggctg gaaaaacttg
     5581 ctgattatca ggaagggttg gcagaaccga tgcaactggc ctcggatcct ctctccatgc
     5641 gtatttatac accggaagag tgtgaacgac tggatgccag ctgccgtggg tttctgcttt
     5701 tccttgagca gattcaggtg ctcaaccttg aaactcgtga aatggtgata gagcgagtgc
     5761 tcgcgctgga taacgcagag ttcgaactgg atgatctgaa atgggtgatc ctgatggtgt
     5821 tgttcaacat cccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
     5881 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcaaaa
     5941 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
     6001 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
     6061 gtccgtcctc tgaaatcttc agcggatgga catattgtca aagttctgga ggggcaggtt
     6121 tgccccatat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
     6181 tgcagtaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
     6241 attacatgcc cccaatgtcg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
     6301 acatttcact cttgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
     6361 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
     6421 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
     6481 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
     6541 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
     6601 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
     6661 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgcgca
     6721 gcgtgaaacc attttttccc gctggccagg tccagtcacc tttgtctttc ccgcgcctgc
     6781 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
     6841 tccgttggtg gctgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
     6901 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
     6961 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
     7021 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
     7081 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
     7141 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
     7201 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
     7261 ttgccagagc ggatgagctt actgaacggg cagcgttggc tggtgctgtt aataccctca
     7321 tgcggttaga agatggacgc ctgctgggtg acaataccga tggtgtaggc ttgttaagcg
     7381 atctggaacg tctgtctttt atccgccctg gtttacgtat tctgcttatc ggcgctggtg
     7441 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
     7501 atcgaacggt atcccgcgcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
     7561 agacgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgcaacat
     7621 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
     7681 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
     7741 gaggctcaaa gcgtactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
     7801 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
     7861 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
     7921 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgcg cgatctctgg
     7981 cgagagtctg gcgtatcgct ttactggaga tacgccagaa cagtggttag cgagttttcg
     8041 tcagcatcgc tgggacctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
     8101 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
     8161 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
     8221 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
     8281 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
     8341 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
     8401 atcttcgcca atggttaatg ggttgccatc tgggttgtat gaggatttat gagtgacatg
     8461 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
     8521 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
     8581 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
     8641 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
     8701 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgattgc
     8761 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaaatac ttgtgcaaaa
     8821 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaggatg
     8881 gtcggaacaa cgaagag
//
LOCUS       DAZKFU020000194         8751 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZKFU020000194 DAZKFU020000000
VERSION     DAZKFU020000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.196"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..1150
                     /gene="allD"
                     /locus_tag="ACHOQQ_RS18360"
                     /old_locus_tag="pgaptmp_003672"
     CDS             101..1150
                     /gene="allD"
                     /locus_tag="ACHOQQ_RS18360"
                     /old_locus_tag="pgaptmp_003672"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGU4471879.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1172..2407
                     /gene="allC"
                     /locus_tag="ACHOQQ_RS18365"
                     /old_locus_tag="pgaptmp_003673"
     CDS             1172..2407
                     /gene="allC"
                     /locus_tag="ACHOQQ_RS18365"
                     /old_locus_tag="pgaptmp_003673"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HGU4471880.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLETQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPHVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2418..3203
                     /gene="allE"
                     /locus_tag="ACHOQQ_RS18370"
                     /old_locus_tag="pgaptmp_003674"
     CDS             2418..3203
                     /gene="allE"
                     /locus_tag="ACHOQQ_RS18370"
                     /old_locus_tag="pgaptmp_003674"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HGU4471881.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3332..4477)
                     /gene="glxK"
                     /locus_tag="ACHOQQ_RS18375"
                     /old_locus_tag="pgaptmp_003675"
     CDS             complement(3332..4477)
                     /gene="glxK"
                     /locus_tag="ACHOQQ_RS18375"
                     /old_locus_tag="pgaptmp_003675"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HGU4471882.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEACGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNDIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4499..5800)
                     /locus_tag="ACHOQQ_RS18380"
                     /old_locus_tag="pgaptmp_003676"
     CDS             complement(4499..5800)
                     /locus_tag="ACHOQQ_RS18380"
                     /old_locus_tag="pgaptmp_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HGU4471883.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5857..7218)
                     /gene="allB"
                     /locus_tag="ACHOQQ_RS18385"
                     /old_locus_tag="pgaptmp_003677"
     CDS             complement(5857..7218)
                     /gene="allB"
                     /locus_tag="ACHOQQ_RS18385"
                     /old_locus_tag="pgaptmp_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HGU4471884.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7278..8732)
                     /locus_tag="ACHOQQ_RS18390"
                     /old_locus_tag="pgaptmp_003678"
     CDS             complement(7278..8732)
                     /locus_tag="ACHOQQ_RS18390"
                     /old_locus_tag="pgaptmp_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HGU4471885.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
BASE COUNT         2333 a         2300 c         2123 g         1995 t
ORIGIN      
        1 ctaactatat ttttttgcct gtctggatca cataatccag atattttccc tgtatgttaa
       61 ttgcagtcat gcttcacacc gtcgttaaaa aggaagacag atgaaaatca gtcgggaaac
      121 actccaccag ctaattgaga ataaactctg ccaggctggg ttaaaacgtg agcacgctgc
      181 aaccgtggct gaagtattgg tttacgccga tgccagaggg atccactctc atggtgcggt
      241 gcgcgtggaa tactacgcgg aacgcatttc aaaaggcggc accaaccgcg aaccggagtt
      301 tcgtcttgag gaaaccgggc cgtgctcggc aattttacat gccgacaatg ccgccggaca
      361 ggtcgcggct aaaatgggta tggaacatgc catcaaaacc gcccagcaaa atggcgttgc
      421 ggtggtcggt atcagccgga tgggtcacag cggcgcaatc tcttattttg tgcagcaggc
      481 agcccgcgcc ggattaattg gcatttcgat gtgccagtcc gatccaatgg tggtgccttt
      541 tggcggcgcg gaaatttact acggtactaa cccactggcc tttgccgcgc cgggagaagg
      601 cgacgagatc cttacctttg atatggcgac taccgtacag gcatggggaa aagtgctcga
      661 cgcccgctcg cgtaatatgt ctatcccgga tacctgggcg gtcgataaaa acggtgcacc
      721 aacaaccgat ccgttcgcgg tacatgctct gctccccgca gccgggccga aagggtatgg
      781 cctgatgatg atgattgacg tcctctcagg cgtcttactc ggcttaccgt tcgggcgaca
      841 ggttagttcg atgtatgacg atttacacgc cgggcgtaat ttggggcaat tacatgtagt
      901 tattaacccg aactttttct cctccagcga attattccgt caacatctta gccagaccat
      961 gcgcgaatta aatgccatta cccccgcgcc cggttttaat caggtttatt atcccggaca
     1021 ggatcaggat attaaacaac gcaaagccgc cgtcgaaggc atcgaaattg ttgatgatat
     1081 ttaccagtat ttaatttccg acgcgcttta taacacgtca tacgaaacga aaaatccctt
     1141 tgcgcaataa ttattgagac aggactttct tatgattaca cattttcgtc aagccataga
     1201 agaaacgcta ccctggcttt cctcatttgg cgctgaccca acgggtggga tgacccgttt
     1261 actttattca ccggaatggc tggaaaccca gcagcaattt aaaaaaagaa tggcagcaag
     1321 cgggctggaa acacgtttcg atgaagtggg gaatttatac ggtcgcctga gtggcaccga
     1381 atatccacag gaagtggttc tgagcggttc gcatatcgat accgtggtta acggcggtaa
     1441 cctcgacggg caattcggcg cgctggcggc gtggctggca attgactggc tggaaacgca
     1501 atacggcgca ccgctgcgta cggttgaagt ggtggcgatg gcggaagaag aaggcagccg
     1561 cttcccgtat gttttctggg gcagtaaaaa tatctttggg ctggcgaatc ctgacgacgt
     1621 gcggaatatc tgtgatgcca aaggaaatag ttttgtcgat gcgatgaagg cttgcggatt
     1681 tactctgccg gacgccccgc taactccgcg tcaggatatt aaagcctttg tcgaactgca
     1741 tatcgaacag ggctgtgtgc tggaaagtaa tgggcaatca attggcgtgg tgaatgcaat
     1801 tgtcgggcaa cgtcgctata cggtgacgct gaacggcgaa tcaaaccatg caggcaccac
     1861 gccgatgggt tatcgtcgtg atacggttta cgctttcagt cgcatttgcc atcagtcgat
     1921 cgaaaaagcg aaaaagatgg gcgacccgct ggtgctgacc tttggtaagg tggagccgcg
     1981 cccgaatacg gtgaatgtgg tgccgggtaa aaccacattc accattgatt gtcgtcatac
     2041 cgacgccgcc gtgctgcgcg atttcactca gcagttagaa aacgacatgc gggcgatttg
     2101 cgatgaaatg gacattggta ttgatatcga tttatggatg gacgaagaac ccgtgccgat
     2161 gaataaggac ctggtcgcca ccctgacaga attgtgtgaa agtgaaaaac tgaattaccg
     2221 ggtgatgcac agtggtgccg ggcacgacgc gcagattttc gcgcctcacg tgccgacctg
     2281 catgattttc attcccagca tcaacgggat cagccataac ccggcggaac gcaccaatat
     2341 taccgacctt gccgaagggg tcaaaacgtt ggcactcatg ctttatcaac ttgcctggca
     2401 gaaataagga gtcataaatg ggatatttaa ataacgtcac cggttaccgc gatgatttac
     2461 tggctaaccg tgcgattgtg aaacacggta atttcgcact gttaacacca gacggtctgg
     2521 taaaaaatat tattccgggc tttgaaaatt gtgacgcgac aatcctctcc acgccaaagc
     2581 tgggtgcctc ttttgttgat tatctggtca cactgcatca aaacggtggc aaccaacagg
     2641 gcttcggtgg cgaaggcatt gaaacgttcc tgtatgtgat ctctggaaat atcactgcca
     2701 aagccgaagg caaaacattt gccttaagcg aaggtggcta tctttattgc ccgccaggct
     2761 ccttaatgac gtttgttaac gcccaggccg aagacagcca aatcttttta tataagcgcc
     2821 gctatgttcc ggtagaaggc catgcaccgt ggctggtttc tggcaatgcc agcgaactgg
     2881 aacgcattca ttatgaaggt atggatgatg tgatcctgct ggattttctg cccaaagagt
     2941 taggttttga tatgaacatg catatcctct cttttgcacc aggtgccagc catggttata
     3001 tcgaaacaca cgttcaggaa cacggtgcct atattctttc cggtcagggg gtttataacc
     3061 tcgacaataa ctggatcccg gtgaaaaaag gcgattacat ctttatgggc gcttattctt
     3121 tacaggctgg ttatggcgta gggcgtggtg aagcgttcag ctatatttac tcgaaagatt
     3181 gcaaccgcga cgtagagatt taagtcatac cactcgcctg attattttta gctatgttgg
     3241 attttgccac ggtttatacc gtggcttttt ttgtagtaaa ttcaggtaaa aaaagatgcg
     3301 atataaatcg tatccgcttt aaaggtaagg gtcagttttt aattccctga cctattttaa
     3361 tggcgcaggc aatatttcgc gcgctgttga agagattggt ttcaccgctg gcgagcactt
     3421 cggctaaagg tgccagacga ggcaaaatgc tgaataccgc gtcaatgccg tactggtgca
     3481 ccacttccac gccatcaccc aatacgccag caatcccaat caccggtaca ttaaactgct
     3541 tcgccaccga cgcaacgccc agcggcgctt taccgcctgc cgtttgcgag tcaatgcgcc
     3601 cttccccggt gatcaccagt gctgccccct gcaccgcctg ctccaggttg accgcacgca
     3661 gtacaatctc aatacccggt ttaatatccg cattgagaaa taccgccgcc gcaataccca
     3721 taccgcccgc agcgccgccg cccgccatct gacagacatt aatttcagtt tgctgttgta
     3781 aaacacgggc gtaattttgc agcccctgtt ccagctcttc gaccatctcc ggcgttgccc
     3841 ctttttgcgg gccaaacacc gccgccgcgc cgcgtgcccc taccagcggg ttatcaacgt
     3901 cacatgctac ttcaatatgg cagttcgcca gacgcggatc gcattcctcc atctcaatgc
     3961 ttgccacgcg cgctaaattg ccgccgttag ctgccagcac ctgaccgtcg gcatcgagaa
     4021 aacgcacacc aagcgcctgc gccatgccca taccgccgtc gaccgtcgca ctgccgccaa
     4081 tgccgagaat aatatggcga atgtcgttat ccagcgcatg ccgaattaac tcccccgtac
     4141 caaaactgga ggccagcaac ggattacgct tttcaggcgc gaccagcatc aggccacttg
     4201 ctgccgccat ctcaatcacc gctgttttcc cgtcgccggt aaggccataa aaagcattca
     4261 ctttttcgcc catcggcccg caggcttcaa gcgtcacgat gttaccaccc gtcgcggcga
     4321 ccatcgcatc caccgtccct tcgccgccat ccgctatcgg caaacagata tagttcgcat
     4381 cgggaaagag ggtcgaaaac ccggctttaa ttgcctgaca acatttttct gcacttaagc
     4441 tctctttaaa agagtctggc gcaatgacaa tcttcattat caatcctttt tcaacaaatt
     4501 attcgatgcg ttcccacgga ataaggttat ccataagaac agccagtaaa atcccgacca
     4561 ataagccgtt actgagcaga ggacgaagcg ttaatggaag gtcttgcaga tacacaggcg
     4621 gtaatgccat taaaaatatg ccgacaaata acggcaatgc gagtcgataa atattgcgag
     4681 cggtaaacgt tatttgctgg ctaaacacca gcgcggaaaa gagtaaaggc agataagaaa
     4741 ccagcatgac cgcactactc acgggtaaag ggatactgca aaacagtcgc gtgagtgccg
     4801 gaaccagcgc caccagcagg caaataacgc tgccataaat aaacgaacgc cgcgtgtaat
     4861 cgccagtctg ggttaataaa ccaatggatg aaacaaacgg tgaaaatgga attaccgcca
     4921 gcggtacggt tatcagtgtc ataaatccgg tcgccacaaa gctacgacga taacgcgtat
     4981 tccccgcgcc ctgctgcgga taaaaaacat ccgtgccccg aatcgcaccg taggtattgc
     5041 tgatatttac cagacctgta atcaccgccg tcagaataat tcccggcgac aaagcaccgc
     5101 cactgccgag cgggaaccac tgccagtgca actcaccgga gagcgaatgc gaagaaggaa
     5161 agcaaaagta ccacaacaac cagccggtta tggtgccgac cagcaggcca taacgggcaa
     5221 aacgttgcgg cagaaagata atcatcgcca gtaccaggca catcaccgcc accgagagcg
     5281 cgaatggtgg taactgaatt ttaaaatttg ggtcggctat gccaaacggc agcccgagca
     5341 tacctttgaa aaagatagtg gtcagctgtg cgcccagcat caacataaac aagaccatca
     5401 ccgacggcgt aaataaccgt gccaggcgat ggcctaatcc gctaaaacca atcaacatcg
     5461 tcagcacgcc ggagagcgca atccccactg ccaggctggt ggcgatatcg ttgatcggtg
     5521 tcccgcgcga tgcttcgcca agggtgatag ttaggatggt tccccaccac aggccccccg
     5581 gcccttccat aatagcgcga cgatggccac agaacgcctg agcgaagcag gccagtgcgg
     5641 tagcaagaaa agcatattgt gtgagtgtaa gcaggctact ttgcggcaac tgaaaagcag
     5701 aaagtagcgt aggaggaacc acaaccgtgt tgcaaaaaat aaagaaaaac cactgaaatc
     5761 ctgataacag gctttcgcgg ctgactgcaa aattgaacat aaacacacct taaccggaga
     5821 atgcccgccg caaggacggg cattgcaggg gccagattac tgctgatgtt taaggataaa
     5881 ttgacctttc ggcgcaacag ggaagccctg ttcgatgtcg taaatcacat caccacgtaa
     5941 gatggttttc gtgatacgcg cgccaatggt acggccaaca tacgggctga ctttgtggcg
     6001 atattccaga tcgtcattgg taagaacata gctgctattc ggctgaatga agacgaagtc
     6061 ggcatctttt cctggggcga tacggccttt ttgctgcaga ccgaaaatat ctgctgcgtt
     6121 agtcgccatt aatttgccga acattggcag agacattccg cgtttctgta ccgcttcatc
     6181 gaacatcacg tccatacagt tttgcagacc ggcaataccg ccccatgctt ccatgatgtt
     6241 gccggctttc atttccggag ggcatggtga gtggtcggaa accaggcagt cgatttcacc
     6301 gttaaacagt ttttcccaca tgcctttctg gttttccaga tcgcggatcg gcggtgaaca
     6361 cttcgccaga gtgccgattt cttcgaactg atcggtatcc agtacaaagt aatgcgggca
     6421 ggattcacaa gtaacatcct gaccttcctg acgtgcacga gtcacttctt caacgccttc
     6481 cgggctgctg atatggcaaa cgtgcagacg gcaaccggca actttcgcca gatacagcac
     6541 gcggcgaatc gcttccactt cggtaaatac cggacgcgaa gccacatagt catgggcagt
     6601 tacgcgacct tcacgtttcg cttcttcgcc cagtgcgtca cagatcagcg cgttttcgca
     6661 gtgcaccagc accggctgcc ccagttcgcc cagcttctgc gcgcctttga agaactgcca
     6721 gtcatttaca tcacggaagt cgttgtcgat accgcgatcg ccacaggtcg caacgaagca
     6781 tttgaagccg acaacgccca cttcatccag ctcatgcaga cggtcgatgt tgtaagacac
     6841 caggccaccg agttgtgccg catcgatagt cagcttgcct ttagcggcat cgaacttcag
     6901 ttcaattgaa gcgcggtcaa ccgttgcagg cagctggttg agcggcattt cgatcatggt
     6961 ggtgatacca ccttttgccg ctgcgcgagt accggtttca taaccttccc agtggctacg
     7021 acccggttca gaaatatggg tgtgcgcatc aaccatgccc ggcgaaacca ccagaccaga
     7081 cgcatccata acttcttttg catcgcccag atcctgaccg atagcagcaa ttttgccgcc
     7141 tttaacggcg atatctacaa cgcgagcttc gttttctaaa ataacggtgc cgtttttaat
     7201 gattaaatca aaagacataa caaactcctt gtaaaataca gattggtttt taactcaatg
     7261 ttcaatatcg ggattaatta acctatggtt ttttgctctc ctgttttttc tgctgttgta
     7321 cgtttcttta ataaggcgta ggccgcaaag gcgacgatga cgccgacaaa ccatgagaca
     7381 cgcgataacg gttccataaa gggaataaac ttaccgccaa gagataaaat aacggccacc
     7441 agagttactg aaaacgcggt gaggttaaaa ccgttatcgt aatatttata atcgccaggt
     7501 gcggtgtaca gttcatcaag attaatttgc ccacgcatca ccacaaaata atgcgccatc
     7561 atgacaccaa ttaccggacc aagcattccg ccgataatat cgaggaacag ataaatactg
     7621 tcctgatttt ccattaattt ccacgggcag atcagcaggc tgataatact ggcaatcagt
     7681 acgccgtttt tataggtcag ttttgtcggt gcaatggcag caatctgata accagccgga
     7741 ataatattac cagtggcgtt agtggagata gtcgtcatca gaataaccag taccgcaaag
     7801 aacgaggcaa acaggctgtc ccaacgctga acaatatcca gcacgttcca ggtatcagcg
     7861 ccgtaatgaa tactggctcc ggcaataata cataccccgg cgaccgcaaa cagaatatag
     7921 gccacaacta aacccagcgt ttgccccagc gcttgctcac gaaacgagtg ggcgttttgc
     7981 gtaaagtcgg atgcgctcac cgccggagcc gcccagaccg caactaccgc gttaatcacc
     8041 accaggaaca ggaagccact gttttctgct ttctgaatac cgctcggaat gtagtcaaag
     8101 attggaccga gcccgaccag cgaaatcgcc caaatcgcca taccgccgaa aacgatatag
     8161 atgcacgggt taagaatggc agtgaattta tttaaaactt tgccaccgcc aaaacctata
     8221 ccaacgttga ccagccagaa gagtaagaaa gtaattaagc ccggtagaga aaggcctaac
     8281 agagtgaaat caccaccgag agtcaaaaat cccggccaga ttttgccaat cagaatcaag
     8341 caggccagtg accccgcgta acattgcagg ccaaaccaca taatggcagc aataccgcct
     8401 cttaataatc cgggaaacag tgcgccacgt acgccgtaag aagcacgcag gatcatggca
     8461 aacggtacgc cgtatttact gcccgcagcg ccgtttaata ccattaccgc ggcaatgaaa
     8521 aaggcgctga ggataattgc cagcataata ctaaaggtag acaagccgag aataaaaaag
     8581 ccgccgacca tcacataatt gggaacgtta tgaaccgaac ccatccataa ggtaaaatag
     8641 ttaaatgttt tccaggtccg ctggctttgc gttttcggca atagatcttc gctatagccg
     8701 cgttgctgga atagttttct ctgatgttcc ataaatccct cttaatttca c
//
LOCUS       DAZKFU020000195         8745 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZKFU020000195 DAZKFU020000000
VERSION     DAZKFU020000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.197"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(318..530)
                     /gene="rpmE"
                     /locus_tag="ACHOQQ_RS18395"
                     /old_locus_tag="pgaptmp_003679"
     CDS             complement(318..530)
                     /gene="rpmE"
                     /locus_tag="ACHOQQ_RS18395"
                     /old_locus_tag="pgaptmp_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGU4471886.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            733..2931
                     /gene="priA"
                     /locus_tag="ACHOQQ_RS18400"
                     /old_locus_tag="pgaptmp_003680"
     CDS             733..2931
                     /gene="priA"
                     /locus_tag="ACHOQQ_RS18400"
                     /old_locus_tag="pgaptmp_003680"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGU4471887.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3087..4112
                     /gene="cytR"
                     /locus_tag="ACHOQQ_RS18405"
                     /old_locus_tag="pgaptmp_003681"
     CDS             3087..4112
                     /gene="cytR"
                     /locus_tag="ACHOQQ_RS18405"
                     /old_locus_tag="pgaptmp_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGU4471888.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4204..5163
                     /gene="ftsN"
                     /locus_tag="ACHOQQ_RS18410"
                     /old_locus_tag="pgaptmp_003682"
     CDS             4204..5163
                     /gene="ftsN"
                     /locus_tag="ACHOQQ_RS18410"
                     /old_locus_tag="pgaptmp_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGU4471889.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5256..5786
                     /gene="hslV"
                     /locus_tag="ACHOQQ_RS18415"
                     /old_locus_tag="pgaptmp_003683"
     CDS             5256..5786
                     /gene="hslV"
                     /locus_tag="ACHOQQ_RS18415"
                     /old_locus_tag="pgaptmp_003683"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGU4471890.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5796..7127
                     /gene="hslU"
                     /locus_tag="ACHOQQ_RS18420"
                     /old_locus_tag="pgaptmp_003684"
     CDS             5796..7127
                     /gene="hslU"
                     /locus_tag="ACHOQQ_RS18420"
                     /old_locus_tag="pgaptmp_003684"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGU4471891.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7194..8120
                     /gene="menA"
                     /locus_tag="ACHOQQ_RS18425"
                     /old_locus_tag="pgaptmp_003685"
     CDS             7194..8120
                     /gene="menA"
                     /locus_tag="ACHOQQ_RS18425"
                     /old_locus_tag="pgaptmp_003685"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGU4471892.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8213..8698
                     /gene="rraA"
                     /locus_tag="ACHOQQ_RS18430"
                     /old_locus_tag="pgaptmp_003686"
     CDS             8213..8698
                     /gene="rraA"
                     /locus_tag="ACHOQQ_RS18430"
                     /old_locus_tag="pgaptmp_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGU4471893.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         2117 a         2277 c         2397 g         1954 t
ORIGIN      
        1 agcaggggat tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccacg
       61 ccggtggggg caccgatgcg caccccggct gaaccctgga caatgctacc gccatactgc
      121 gtcatgtcgc cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttgaaataaa
      181 taagaaccag aagcctagcg ccagtcaaca acaggaaatg ataatgagtt aacaccccgg
      241 aatttttcag aatttataaa aggcaaatta caggcacaaa aaaagcgccg tgcggcgctt
      301 ttttcggaaa tccggtctta tttgctgccc gggatgttga aacgcttgtt gaagcggtca
      361 acacggccac cggtagcaac atcacgctgt ttgccagtga agaacgggtg gcacttgctg
      421 cacacgtcga ggttcaggtc atgaccaacg gtggagcgga ttttcattac gttaccgcaa
      481 gagcagctag cagtaatttc ttcgtatttc gggtgaatat cttttttcat gggaaaacct
      541 cggtttaagg ccgcgtcgct cttccagccc taacgccaga caccacgcga tgttaaaagt
      601 atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc atacagaaat taaccagcgt
      661 atgcaaactg atccgcactc ttctacggca atgtgtatac taacccaccg aatttcaagt
      721 caggatgatg ctatgcccgt tgcccacgtt gccttgcccg ttccgcttcc tcgtaccttt
      781 gactatctgc tgccagaagg catgacggtt aaagctgggt gtcgcgtgcg cgtgccgttt
      841 ggcaaacagc aggagcgcat cgggattgtg gtatcagtta gcgatgccag cgaactgccg
      901 ctcaatgagc taaaagcggt agtcgaagtg ctggatagtg agccggtgtt tactcactcc
      961 gtctggcgat tgctgctatg ggcggcagat tattatcatc atccgattgg cgatgtgctg
     1021 tttcatgcct tgccgatttt actacgccag gggcggcctg cggcgaacgc gccgatgtgg
     1081 tactggtttg ccactgaaca aggccatgcg gtggatctga acagcctgaa acgttcgcca
     1141 aagcaacaac aggcactggc ggcattgcgg caaggcaaaa tctggcgcga ccaggttgcc
     1201 acgcttgaat ttaatgatgc cgcgttacag gcgctgcgca aaaaaggtct gtgtgattta
     1261 gcaagtgaaa caccagagtt tagcgactgg cgaacgaact atgccgtttc tggtgagcgg
     1321 ttgcgattga ataccgaaca ggccaccgcc gttggcgcaa ttcatagtgc ggcggatacc
     1381 ttctctgcct ggctgctggc gggtgttaca ggttccggta aaacagaggt ttatttgagc
     1441 gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc
     1501 ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta atgcccccgt agaagttctg
     1561 cattccggcc tgaacgacag cgagcgtctt tcggcgtggt tgaaagcgaa aaatggtgag
     1621 gcggcgattg tgatcggcac ccgctccgcg ctgtttacgc cgtttaaaaa tctcggtgtg
     1681 attgtcattg atgaagagca cgacagttcc tacaagcagc aggaaggctg gcgctatcat
     1741 gcccgcgacc tggcggttta tcgtgcgcac agcgagcaaa tcccgattat tcttggctcc
     1801 gcaacacccg cactggaaac gttatgcaac gtccagcaga aaaaataccg cctgctgcgc
     1861 ctgacgcgtc gggcggggaa tgcgcgtccg gcaattcaac acgtgctgga tttaaaaggt
     1921 cagaaggtgc aggcggggct ggctccggcg ttaatcaccc gtatgcgcca gcatttacag
     1981 gccgacaacc aggttatcct ctttcttaac cgccgtggct ttgcgcctgc cttgctgtgc
     2041 cacgactgcg gctggattgc cgaatgtcca cgttgcgatc actactacac gctgcatcag
     2101 gcgcaacagc atttgcgctg tcaccactgt gacagccaac gcccggtgcc gcgccagtgc
     2161 ccttcctgcg gttccacgca cctggtaccc gtggggctgg gcaccgaaca gcttgaacag
     2221 acgctcgcgc cgttattccc tggtgtgccc atttctcgta tcgaccgcga taccaccagc
     2281 cgcaaaggag cgctggaaca acaactggcg gaagtgcatc gcggcggcgc gaggatcttg
     2341 attggcacgc aaatgctggc gaaaggtcac cactttccgg atgtgacgct ggtagcgtta
     2401 ctggatgtgg acggcgcgct gttttctgcc gattttcgct cggcagagcg ttttgcccag
     2461 ctatacaccc aggtagcagg ccgtgccggg cgtgcgggta aacagggcga agtggtgctg
     2521 caaacgcatc atccggaaca cccgctattg caaacgttgc tctataaagg ctacgacgct
     2581 tttgccgaac aggcactggc tgagcgacgc atgatgcagc tgccgccgtg gaccagccat
     2641 gtgattgtgc gtgcggaaga tcataacaat cagcatgcgc cgctgttcct gcaacaactg
     2701 cgtaatctga tcctctccag cccgctggca gacgacaaat tgtgggttct cggtccggtt
     2761 ccggcactgg cacctaaacg tggcggtcgc tggcgctggc agatattgtt gcagcaccct
     2821 tcccgcgtgc gcttgcaaca catcattaac ggtacgctgg cactcatcaa tacaataccg
     2881 gattcccgta aggtgaaatg ggtgctggat gttgatccga ttgagggtta aaccgctcac
     2941 gatgcgaggc ggatcgaaaa attcaatatt catcacactt ttcatgaaaa ttctgtaacc
     3001 gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg
     3061 cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca
     3121 tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac cgtctcccga gcattaatga
     3181 atcccgataa agtctctcag gccacccgta atcgggttga aaaagcggcc cgggaagtgg
     3241 gttatttacc gcagcctatg gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg
     3301 tgattgtccc ggatatctgc gatcccttct ttagcgaaat tattcgcggt atagaagtta
     3361 cggcggcaaa tcacggatat ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg
     3421 aaaaaacctt tatcgatttg atcatcacca agcaaattga tggcatgttg ctgctgggtt
     3481 caaggctgcc gtttgatgcc agcattgagg aacagcgtaa tctgccgccg atggtgatgg
     3541 cgaacgaatt tgcaccggaa ctggagctgc ctaccgttca tatcgacaat ctgaccgccg
     3601 catttgatgc agtaaattat ttatatgagc aagggcataa acggattggc tgtatagccg
     3661 gtcccgaaga gatgccgctg tgtcactacc gcctgcaagg ttacgttcag gcgctgcgtc
     3721 gctgcggcat tatggttgat ccgcaataca tcgcccgtgg tgacttcacc ttcgaagccg
     3781 gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc gcctactgct gtcttctgcc
     3841 atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc
     3901 cggaagacct ttccataatc ggttttgata acatcgacct gacgcaattt tgtgatccgc
     3961 cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc
     4021 ttgatcaaat gcaggggcaa cacgttggca gtggctctcg tttaatggac tgcgaactta
     4081 tcatccgggg atcaacacgc gcgttacctt aaagtaaacc tttaagactg ccgtgactgg
     4141 tcaaaggccc gccgcttaag taacatggcg ggctgacgaa cgaataaata cagcgaaacg
     4201 atagtggcac aacgagatta tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag
     4261 agcacctcac ggaaaaagca acgaaatctg cctgcggttt ctcccgctat ggtcgctatt
     4321 gctgccgccg ttcttgtgac ctttatcggt ggtctgtact tcattacgca tcacaagaaa
     4381 gaagagtccg agacgctgca aagccagaaa gtgaccggaa acggactacc accaaaacca
     4441 gaagaacgct ggcgctacat caaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc
     4501 acagaacctt ctgctggtgg tgaagtgaaa acgccggagc aactgacacc agaacaacgt
     4561 cagcttcttg aacaaatgca ggctgatatg cgccagcagc caacgcagct ggttgaggtt
     4621 ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc tacagcgcca acgtcaggcg
     4681 cagcaattag cggaacagca acgcctggct cagcagtcgc gtacgactga acaaagctgg
     4741 cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa
     4801 ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa cccctgcgca cacgaccgcg
     4861 cagtcgaagc cgcagcaagc tgcgccggtt actcgtgccg ctgacgcgcc aaaaccgacg
     4921 gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa
     4981 caggcagaga cggtacgtgc tcagctggcg ttcgaaggct ttgactcaaa aattaccacc
     5041 aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc
     5101 accctcaatc ggttgaagat ggcgggtcat acaaactgca ttcggctcgc cgccgggggt
     5161 tgaaaccctc aaaatccccc ccatctataa ttgcattatg ccccgtactt ttgtacgggg
     5221 tttgtactct gtattcgtaa ccaaggggtc agctcgtgac aactatagta agcgtacgcc
     5281 gtaacggcca tgtggtcatc gctggtgatg gtcaggccac gttgggcaat accgtaatga
     5341 aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg
     5401 gcggtactgc ggatgctttt acgctgttcg aactgtttga acgtaaactg gaaatgcatc
     5461 agggccatct ggttaaagcc gccgttgagc tggcaaaaga ctggcgtacc gatcgcatgt
     5521 tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac cgcttcgctt atcatcaccg
     5581 gtaacggtga cgtggtgcag ccagaaaacg atcttattgc tatcggctcc ggcggccctt
     5641 acgcccaggc tgcggcgcgc gcgctgttag aaaacactga acttagcgcc cgtgaaattg
     5701 ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta taccaaccat ttccacacca
     5761 tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa
     5821 tcgtcagcga actggataag cacatcatcg gccaggacaa cgccaagcgt tctgtggcga
     5881 ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga agagctgcgc catgaagtga
     5941 ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc
     6001 gtctggctaa gctggcgaat gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag
     6061 tgggctacgt cggtaaggaa gtggattcta ttattcgcga tctgaccgat gccgccgtga
     6121 aaatggtacg cgtccaggct atcgagaaaa accgttatcg cgctgaagaa ctggcagaag
     6181 aacgtattct cgacgtgctg atcccacctg ctaaaaacaa ctggggacag accgaacagc
     6241 agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg
     6301 atgacaaaga aatcgagatc gatcttgccg cagcaccgat gggcgttgaa attatggctc
     6361 ctccaggcat ggaagagatg accagccagc tgcagtccat gttccagaac ctgggcggtc
     6421 agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat gaagctgctg attgaagaag
     6481 aagcggcgaa actggtgaac ccggaagagc tgaagcaaga cgctatcgac gctgttgagc
     6541 agcacgggat cgtgtttatc gacgaaatcg acaaaatctg taagcgcggc gagtcttccg
     6601 gtccggatgt ttctcgtgaa ggcgttcagc gagacctgct gccgctggta gaaggttgca
     6661 ccgtttccac caaacacggg atggtcaaaa ctgaccacat tctgtttatc gcttctggcg
     6721 cgttccagat tgcgaaaccg tctgacctga tcccggaact gcaaggtcga ctgccaatcc
     6781 gcgttgaact gcaggcactg accaccagcg acttcgagcg tattctgacc gagccgaatg
     6841 cctctatcac cgtgcagtac aaagcactga tggcgactga aggcgtaaat atcgagttta
     6901 ccgactccgg tattaaacgc atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa
     6961 acatcggtgc tcgtcgttta cacactgttc tggagcgttt aatggaagag atttcctacg
     7021 acgccagcga tttaagcggt caaaatatca ctattgacgc agattatgtg agcaaacatc
     7081 tggatgcgtt ggtggcagat gaagatctga gccgttttat cctataatcg cgttcaatca
     7141 ttttcatcat tgtttgatgg ggctgaaagg ccccattttt attggcgcgt attatgactg
     7201 aacaacaaat tagccgaact caggcgtggc tggaaagttt acgacctaaa accctccccc
     7261 tcgcctttgc tgcaattatc gtcgggacgg cgctggcatg gtggcaaggt cattttgatc
     7321 cgctggtcgc cctgctggca ctgattaccg ccgggctatt acagatcctt tctaacctcg
     7381 ccaatgatta cggcgatgca gtaaaaggca gcgataaacc tgaccgcatt gggccgctac
     7441 gcggcatgca aaaaggggtc attacccagc aagagatgaa acgggcgctc attattaccg
     7501 tcgtgctcat ctgtctctct gggctggcac tggttgcagt ggcatgccat acgctggctg
     7561 attttgtcgg tttcctgatt cttggcgggt tgtcgatcat tgccgctatc acctacaccg
     7621 tgggcaatcg tccttatggt tatatcggtc tgggtgatat ttccgtactg gttttctttg
     7681 gctggttaag cgtcatgggg agctggtatt tacaggctca tacattgatt ccggcactga
     7741 tccttccggc gaccgcatgc ggcctgctgg caacggcagt actgaacatt aataacctgc
     7801 gtgatatcaa tagcgaccgc gaaaacggca aaaacacgct ggtggtgcgc ttaggtgaag
     7861 tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc tctggtatgt ctggcgctgt
     7921 ttaatctctt ttcgctgcat agcctgtggg gctggctgtt cctgctggcg gcaccgttgc
     7981 tggtgaagca agcccgttat gtgatgcggg aaatggaccc ggtggcgatg cgaccgatgc
     8041 tggaacgcac tgtcaaggga gcgttactga ctaacctgct gtttgttgta gggatattcc
     8101 taagccagtg ggcagcataa ctgacaaata tcaattaaca attgatgatt ttgccaacag
     8161 accacatagc gcgatatact gaaaattctc gcagcaactg aatgttaagc ctatgaaata
     8221 cgatacttcc gagctttgtg acatctatca agaagatgtt aacgtcgtgg aaccgctgtt
     8281 ctccaacttt ggcggacggg cgtcgtttgg cggacaaata atcacggtaa aatgtttcga
     8341 ggacaacggg ttgctgtacg atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt
     8401 cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt
     8461 acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg cgtcaggtag atgacctgga
     8521 agagttggat atcggcatcc aggcgatggc ggcaattccg gttggtgccg ctggcgaagg
     8581 cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtt accttcttct ccggcgacca
     8641 tctttatgcc gacaataccg ggattattct ttcagaagat ccgctggata ttgaataagt
     8701 atctgccatg cgttaaaagg ggaattaaat tccccttttt tatac
//
LOCUS       DAZKFU020000196         8596 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZKFU020000196 DAZKFU020000000
VERSION     DAZKFU020000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.198"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(272..1936)
                     /gene="glnS"
                     /locus_tag="ACHOQQ_RS18435"
                     /old_locus_tag="pgaptmp_003687"
     CDS             complement(272..1936)
                     /gene="glnS"
                     /locus_tag="ACHOQQ_RS18435"
                     /old_locus_tag="pgaptmp_003687"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGU4471894.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2139..4085)
                     /gene="nagE"
                     /locus_tag="ACHOQQ_RS18440"
                     /old_locus_tag="pgaptmp_003688"
     CDS             complement(2139..4085)
                     /gene="nagE"
                     /locus_tag="ACHOQQ_RS18440"
                     /old_locus_tag="pgaptmp_003688"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGU4471895.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATTAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4418..5218
                     /gene="nagB"
                     /locus_tag="ACHOQQ_RS18445"
                     /old_locus_tag="pgaptmp_003689"
     CDS             4418..5218
                     /gene="nagB"
                     /locus_tag="ACHOQQ_RS18445"
                     /old_locus_tag="pgaptmp_003689"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4471896.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5278..6426
                     /gene="nagA"
                     /locus_tag="ACHOQQ_RS18450"
                     /old_locus_tag="pgaptmp_003690"
     CDS             5278..6426
                     /gene="nagA"
                     /locus_tag="ACHOQQ_RS18450"
                     /old_locus_tag="pgaptmp_003690"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4471897.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6435..7655
                     /gene="nagC"
                     /locus_tag="ACHOQQ_RS18455"
                     /old_locus_tag="pgaptmp_003691"
                     /gene_synonym="nagR"
     CDS             6435..7655
                     /gene="nagC"
                     /locus_tag="ACHOQQ_RS18455"
                     /old_locus_tag="pgaptmp_003691"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGU4471898.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7703..8455
                     /gene="nagD"
                     /locus_tag="ACHOQQ_RS18460"
                     /old_locus_tag="pgaptmp_003692"
     CDS             7703..8455
                     /gene="nagD"
                     /locus_tag="ACHOQQ_RS18460"
                     /old_locus_tag="pgaptmp_003692"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGU4471899.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         2107 a         2256 c         2199 g         2034 t
ORIGIN      
        1 tttatggata aattgcgaag cggaattaat tcgtgacgaa aagcagaaaa aaacgccgca
       61 gaccgcggcg tttaggattg tttgcatgtc tcttgccgga tgcggcgtga acgccttatc
      121 cggcctacaa aagcacgcaa actcaatata ttgcagagat catgtaggcc tgataagcgt
      181 agcgcatcag gcaatttagc gtttgaaact gtttgtctgt tccttgccgg atgcgacgta
      241 aacgccccat ccggcatagc gaaacttaaa attactcgcc tactttcgcc caggtatcac
      301 gcagcccaac ggtgcggtta aataccggtt tttccgccgt agaatggcgg ctatcgaggc
      361 agaagtaacc ttcacgctca aactggaatg ctttacccgc taccgcatct ttcagcgacg
      421 gttcagcaaa gccctgtttg atcaccagcg attccgggtt aatcaccgac aggaaatcat
      481 ccgcagcacc tgggttaggc acgctgaaca gacgatcata caaacggatt tcaaccggca
      541 gcgcatgtgc cgcgctcacc cagtgaataa cgcctttgac tttacgacca tcagccggat
      601 ctttgcttaa ggtgtcggcg tcataagtac agaagatggt ggtgatatta ccttcggcat
      661 ctttctcgac gcgttctgcc ttaatcacat aagcattacg cagacgcact tctttaccga
      721 gcaccagacg tttgtactgc ttgttagctt cttcgcggaa atcggcgcga tcgatccaaa
      781 tctcaccgct aaacggcacc tgacggctgc ccatttccgg tttgttcgga tggttcggca
      841 tggtaaccat ttcgccttcg ccctgatagt tttcgataac cagtttcacc ggatcgataa
      901 ccgccattgc gcgcggcgca ttttcgttga gatcttcacg gatgcaggat tccagcgacg
      961 ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg tttgcagaac tcacgaatag
     1021 aagccgcagt gtaaccacga cgacgcagac cggaaatggt cggcatacgc gggtcatccc
     1081 agccttcaac gtgcttatcg gtcaccagca ggttcagctt acgcttggac atcacagtgt
     1141 attccagatt caggcgcgag aactcatact ggcgcgggtg aacaggaatg gtgatgttgt
     1201 ccagtaccca gtcgtacaga cgacggttgt cctggaactc cagcgtacac agagagtgcg
     1261 taataccttc cagcgcatcg ctgatgcaat gggtgaagtc gtacatcggg tagatgcacc
     1321 acttgttgcc agtctggtgg tgttcagcaa atttaatgcg gtacagcacc ggatcgcgca
     1381 tcacgataaa cggtgaagcc atgtcgattt tcgcacgcag gcaggcttta ccttcttcaa
     1441 aaccgccggt acgcattttc tcgaacagcg ccaggttctc ttcaacgcta cggtcgcggt
     1501 acgggctgtt tttaccaggc tgcgtcaggg tgccgcggta ttcgcggatc tgttccggcg
     1561 tcagttcatc aacgtacgcc aggcctttat tgatcagttc gatcgcatag gcgtggagct
     1621 gatcaaaata atcggaggag taacggacgt taccagacca gtgaaaacct aaccactcaa
     1681 cgtcgttttt gatcgaatcg acatactcga tatcttcttt taccgggtta gtgtcgtcga
     1741 aacgcaggtt gcactggcct ttatagtcct gggcgatccc gaagttcagg cagatagatt
     1801 tcgcatggcc aatatgcaga tagccattcg gctccggcgg gaaacgggtg tgtactgtgg
     1861 tgtgcttacc actggccaga tcttcatcga tgatctgacg gataaagtta gtcgggcggg
     1921 cttctgcctc actcatcgtg gattcctcaa agcgtaaaca acgtataacg gcgtatgatc
     1981 ttataagcgg gacaggctga caactgttag ttagtgaaaa tcgaaaatct cactgagatt
     2041 atggggatga ttgatgaaaa aaaaagcggc agagaagatc tccaccgctt gtcattgttg
     2101 gatgcgacgc tcaagcgtcg catcaggcat aaagcagatt actttttgat ttcatacagc
     2161 ggtgtttgac ccgccacaac atggccctga gctttaatga tcaagccact gaaatcgtcg
     2221 atattactgc aaaccactgg gctaatcatc gagcgggcgt tagcgttcag gtaatccaga
     2281 tccatttcca gaatcggttg tcctgcgctt acctgcgcgc cctcttccac cagacgttta
     2341 aagcctttac cttccagcgc tacggtgtcg atacccatat ggacgacgat ctccgcgcct
     2401 ttttcggttt ccagacagaa cgcgtggttg gtgttgaaga ttttcacgat tgtccctgcg
     2461 gctggtgata cgacgatttt atctgtcggt ttcaccgcca caccgtcacc caccgctttg
     2521 ctggcgaatg cttcgtcagg aacctgatcc agtgccacga catcaccggt aatcggcgat
     2581 accagctccg cgatagatac cgcgtttggt acagcctgcg gttttgctac aggagcggta
     2641 gttgccggag cagtttcagc agaggcagct gctaccggac cgcgggcaac gactttcttc
     2701 atcgcatcgc cgatggattc tgctttcgcg ccaacaatca cctgaatagt ctgtttgttc
     2761 agttttacta ccccagaagc ccccagacgt ttacacatcg tatcgttaac gcgggcagag
     2821 tcagccacgg taaggcgcag acgggtgata caggcgtcaa ttgctttcag gttgtcagtg
     2881 ccgccaaccg cagcaatata gttggtcgcc agttgattga ggccttcttc agtgttgctg
     2941 ttggcttctt cagtaacgat ctcgtcttct ttatcttcac gacccggcgt tttcaggttg
     3001 aacatgcgga taaccaaact gaacaccacg aagtagatag cgaagaagac aacacccata
     3061 accagcagca tccagacgtt ctggctggcg gccggcaggt tatacatcaa cgcgtagtcg
     3121 atagcccccg cagagaaaga gaagccggca tggataccca gcagcgttgc cacaaacagg
     3181 ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg gagcaaggaa catgaacagg
     3241 aattccagcg gctcagtcac accggtcagg aacgcagtaa cagcaacaga aagcagcatc
     3301 ccgccaacca tcggacgacg ctctttcggt gctgcgaagt acatcgccag cgccgcaccc
     3361 ggcaggccga acatcatgat cgggaagaag ccggacatga acatccccgc ggtgccgtca
     3421 ccagcgtaga aacggttgat gtcgccgtgg aaaaccgtac ccgccgcgtt ggtgaattca
     3481 ccaatctgga accaggcgat ggtgttcagc acctgatgca gaccggttgg gatcagcaga
     3541 cggttgatga aaccaaagat accggaaccc agcgcgcccg cagaaacgat ccactcgccg
     3601 cctgcatgga tagcgtgctg taccggcggc cagacgtaac caaaaatggc cgccagcacc
     3661 agacagaaga agccggtggc gatcggcaca aagcgtttgc cgccgaagaa gctcaggaag
     3721 tccggcagtt taatatcgga ccaacggtta taggctgcgc caccaaccag accggtaatg
     3781 atacccgcca gtacacccat gttaatttct gggttgatgg tcaccatcgc tttggttaac
     3841 acaaagtaac ctaccgcacc cgccagcgcc gccgcacctg cgctgtcttt cgaccagctg
     3901 gatgccacac cgatggcgaa gattaatgcg aggttatcaa aaatcgcacc gcccgcctgg
     3961 gcaataaacg caacgttaag taaatctggc tgaccgaatc gcagcaacag tgccgccacc
     4021 ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc gctggaaaaa acctaaaata
     4081 ttcatcttat tccccctacg agaaccctat ttggctcgtt taaagccgta tttttattgt
     4141 gctgcaaatt gtactgccga tgttctgtaa tcagattgtt agatcatctg ccacagagtg
     4201 tgtgaaaatt taattcgtat cgcaaattaa acgcgtgtct tttgtgagtt ttgtcaccaa
     4261 atatcgttat catcactccc ttttactggc taaaccagaa aacttatttt atcattcaaa
     4321 aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt tacgcttaaa gatgcctaat
     4381 ccgccaacgg cttacatttt acttattgag gtgaataatg agactgatcc ccctgactac
     4441 cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc aatcgtatca atgcgttcaa
     4501 accgactgct gatcgtccgt ttgttctggg cctgccaact ggcggcacgc caatgaccac
     4561 ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc agctttaagc acgttgtcac
     4621 cttcaacatg gacgagtatg tcggtctgcc gaaagagcat ccggaaagct actacagctt
     4681 tatgcaccgt aatttcttcg atcacgttga tattccagca gaaaacatca accttctcaa
     4741 cggcaacgcc ccggatatcg acgccgagtg ccgccagtat gaagaaaaaa tccgttctta
     4801 cggaaaaatt catctgttta tgggcggtgt aggtaacgac ggtcatattg catttaacga
     4861 accggcgtct tctctggctt ctcgtactcg tatcaaaacc ctgactcatg acactcgcgt
     4921 cgcgaactct cgtttctttg ataacgatgt caatcaggtg ccaaaatatg ccctgactgt
     4981 cggtgttggt acactgctgg atgccgaaga agtgatgatt ctggtgctgg gtagccagaa
     5041 agcactggcg ctgcaggccg cagttgaagg ttgcgtgaac catatgtgga ccatcagctg
     5101 tctgcaactg catccgaaag ccatcatggt gtgcgatgag ccttccacca tggagctgaa
     5161 agttaagact ttaagatatt tcaatgaatt agaagcagaa aatatcaaag gtctgtaatt
     5221 gttatccctg ccctgacacc ttgctcaggg caatattttt taaaatcggg ggtcagaatg
     5281 tatgcattaa cccagggccg gatctttacc ggccacgaat ttcttgatga ccacgcggtt
     5341 gttatcgctg atggcctgat taaaagcgtc tgtccggtag cggaactgcc gccagagatc
     5401 gaacaacgtt cactgaacgg ggccattctc tcccccggtt ttatcgatgt gcagttaaac
     5461 ggctgcggcg gcgtacagtt taacgacacc gctgaagcgg tcagcgtgga aacgctggaa
     5521 atcatgcaga aagccaatga gaaatcaggc tgtactaact atctgccgac gcttatcacc
     5581 accagcgatg agctgatgaa acagggcgtg cgcgttatgc gcgagtacct ggcaaaacat
     5641 ccgaatcagg cgttaggtct gcatctggaa ggcccgtggc tgaatctggt gaaaaaaggt
     5701 acccataatc cgaattttgt gcgtaagcct gatgccgcgc tggtcgattt cctgtgtgaa
     5761 aacgccgacg tcattaccaa agtgaccctg gcaccggaaa tggttcctgc ggaagtcatc
     5821 agcaaactgg caaatgccgg gattgtggtt tctgccggtc actccaacgc gacgttgaaa
     5881 gaagcgaaag ccggtttccg cgcggggatt acctttgcca cccatctgta caacgcgatg
     5941 ccgtatatta ccggtcgtga accgggcctg gcgggcgcga tcctcgatga agctgacatt
     6001 tattgcggta ttatcgctga tggcctgcat gttgattacg ccaacattcg taacgctaaa
     6061 cgccttaaag gcgacaaact gtgtctggtt accgacgcca ccgcgccagc aggtgccaac
     6121 attgaacagt tcatttttgc gggtaaaaca atatactacc gtaacggact ttgtgtggat
     6181 gagaacggta cgttaagcgg ttcatcctta accatgattg aaggcgtgcg taatctggtc
     6241 gaacattgcg gtatcgcact ggatgaagtg ctgcgtatgg cgacgctcta tccggcgcgt
     6301 gcgattggcg ttgagaaacg tctcggcaca ctcgccgcag gtaaagtagc caacctgacc
     6361 gcattcacac ctgattttaa aatcaccaag accatcgtta acggtaacga ggtcgtaact
     6421 caataagaga aagtatgaca ccaggcggac aagctcagat aggtaatgtt gatctcgtaa
     6481 aacagcttaa cagcgcggcg gtttatcgcc tgattgacca gtacgggcca atctcgcgga
     6541 ttcagattgc cgagcaaagc cagcttgccc ccgccagcgt aaccaaaatt acgcgtcagc
     6601 ttatcgaacg cgggctgatc aaagaagttg atcagcaggc ctccaccggg ggccgccgcg
     6661 ctatctctat cgtcaccgaa acccgcaatt tccacgcaat cggcgtacgg cttggtcgtc
     6721 acgacgccac catcaccctg tttgatctca gcagcaaagt gctggcagaa gaacattacc
     6781 cgctgccgga acgtacccag cagacgctgg aacatgccct gctgaatgcc attgctcagt
     6841 ttattgatag ctaccagcgc aaactgcgcg agctgatcgc catttccgtt atcctgccag
     6901 gacttgttga cccggacagc ggcaaaattc attacatgcc gcatattcag gtagaaaact
     6961 gggggctggt agaagcactg gaagagcgtt ttaaagtgac ctgtttcgtt ggtcacgata
     7021 tccgtagtct ggcgctggcg gagcactact tcggtgcaag tcaggattgc gaagactcca
     7081 ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat ctctaacggg cgcattttta
     7141 ttggccgcaa cggtaacgtc ggtgaaattg gccatattca ggtcgaaccg ctgggtgaac
     7201 gctgccactg cggcaacttt ggctgcctgg aaactatcgc tgccaacgcc gctattgaac
     7261 aacgggtgtt gaatctgtta aagcagggct accagagccg cgtgccgctg gacgactgca
     7321 ccataaaaac tatctgcaaa gctgcgaaca aaggcgatag cctggcgtcg gaagtgattg
     7381 agtatgtcgg tcgtcatctg ggcaaaacca tcgccattgc catcaacctg tttaacccgc
     7441 aaaaaattgt tattgccggt gaaatcaccg aagccgataa agtgctgctc cctgctattg
     7501 aaagctgcat taatactcag gcgctgaagg cgtttcgcac taatctgccg gtggtacgtt
     7561 ctgagctaga ccaccgctcg gcaatcggcg cttttgcgct ggtaaaacgc gccatgctca
     7621 acggtatttt gctccagcat ttgctggaaa attaatgtgc ttttatagtg gcgcttattg
     7681 ttgtcaatat tctgggtagt ccatgaccat taaaaatgta atttgcgata tcgacggcgt
     7741 gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa tttttgcacg ggattatgga
     7801 taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg cagaccgggc aagatctggc
     7861 gaaccgcttt gccactgccg gtgtcgatgt acctgacagc gtgttttata cctctgcgat
     7921 ggcgaccgcc gatttcctgc gtcgccagga aggcaagaaa gcgtatgtgg tgggcgaagg
     7981 cgcactgatt catgaactgt acaaagccgg tttcactatt accgatgtga accctgattt
     8041 tgtcattgtt ggcgaaacgc gttcctacaa ctgggacatg atgcataaag cggcctattt
     8101 cgtcgctaac ggtgcacgct ttatcgccac caacccggat actcacgggc gcggttttta
     8161 tccggcctgt ggcgcgttgt gtgcagggat tgagaaaatc tccgggcgca aaccgttcta
     8221 tgttggtaag ccgagtccgt ggattatccg cgcggcatta aacaaaatgc aggcacattc
     8281 ggaagaaacg gtgattgtcg gcgataacct gcgtaccgat attctggccg gcttccaggc
     8341 aggtctggag acgattctgg tgctttctgg tgtttcgtcg ctcgacgata tcgacagtat
     8401 gccgttccgc cccagctgga tttacccatc ggtcgctgaa atcgacgtta tctgaatagc
     8461 aaccacgtct gcggacgtgg ttggctctga tgacaaacgc caaactgcct gatgcgctac
     8521 gcttatcagg cctacgtagc tcctgcaaca tattgaattt tcatgctttt gtaggccgga
     8581 taaggcgttc acgccg
//
LOCUS       DAZKFU020000197         8554 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZKFU020000197 DAZKFU020000000
VERSION     DAZKFU020000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.199"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..1536)
                     /locus_tag="ACHOQQ_RS18465"
                     /old_locus_tag="pgaptmp_003693"
     CDS             complement(34..1536)
                     /locus_tag="ACHOQQ_RS18465"
                     /old_locus_tag="pgaptmp_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGU4471900.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASNEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(1614..2612)
                     /gene="idnR"
                     /locus_tag="ACHOQQ_RS18470"
                     /old_locus_tag="pgaptmp_003694"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(1614..2612)
                     /gene="idnR"
                     /locus_tag="ACHOQQ_RS18470"
                     /old_locus_tag="pgaptmp_003694"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HGU4471901.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRTAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(2679..3998)
                     /gene="idnT"
                     /locus_tag="ACHOQQ_RS18475"
                     /old_locus_tag="pgaptmp_003695"
     CDS             complement(2679..3998)
                     /gene="idnT"
                     /locus_tag="ACHOQQ_RS18475"
                     /old_locus_tag="pgaptmp_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HGU4471902.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(4061..4825)
                     /gene="idnO"
                     /locus_tag="ACHOQQ_RS18480"
                     /old_locus_tag="pgaptmp_003696"
     CDS             complement(4061..4825)
                     /gene="idnO"
                     /locus_tag="ACHOQQ_RS18480"
                     /old_locus_tag="pgaptmp_003696"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HGU4471903.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(4849..5880)
                     /gene="idnD"
                     /locus_tag="ACHOQQ_RS18485"
                     /old_locus_tag="pgaptmp_003697"
     CDS             complement(4849..5880)
                     /gene="idnD"
                     /locus_tag="ACHOQQ_RS18485"
                     /old_locus_tag="pgaptmp_003697"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HGU4471904.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            6097..6660
                     /gene="idnK"
                     /locus_tag="ACHOQQ_RS18490"
                     /old_locus_tag="pgaptmp_003698"
     CDS             6097..6660
                     /gene="idnK"
                     /locus_tag="ACHOQQ_RS18490"
                     /old_locus_tag="pgaptmp_003698"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU4471905.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPHADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(6664..7684)
                     /gene="ahr"
                     /locus_tag="ACHOQQ_RS18495"
                     /old_locus_tag="pgaptmp_003699"
                     /pseudo
     CDS             complement(6664..7684)
                     /gene="ahr"
                     /locus_tag="ACHOQQ_RS18495"
                     /old_locus_tag="pgaptmp_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
     gene            7880..7964
                     /locus_tag="ACHOQQ_RS18500"
                     /old_locus_tag="pgaptmp_003700"
     tRNA            7880..7964
                     /locus_tag="ACHOQQ_RS18500"
                     /old_locus_tag="pgaptmp_003700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7914..7916,aa:Leu,seq:caa)
BASE COUNT         2191 a         2218 c         2006 g         2139 t
ORIGIN      
        1 cgtcgcatca ggcaaagaca aatcgcacct ggcttacctt tttctccccc ccagcaaact
       61 ccccaacatc ccgcgtacaa tctgattcgt cacctggcga gcggcgcttt tggccattgt
      121 ttgcaccaca ccatctttct tcccgccgcg tggtccggta gtgccgaaca aaatatcctt
      181 caatccacca agaatgccgt catctaccgc tacctctttc cctttcgcgg ggggattatt
      241 ttgctgctcg ttactggcct gaaagccttt ttgcaacatc tcataggcgg attcccggtc
      301 cacgtcatcc tcatatttgc catacaccgg ggagtggtta atcaagccat tgcgctcatc
      361 ttccgtcacc ggtcccatcc gcgaacaagg cgcaatcacc atcgcccgtt ccaccacaga
      421 aggacttcct tttgcatcga gaaacgagat taacgcctcg ccggtcccca gttcctggat
      481 tgccttttcg gtatcaaatg ccggattggc ccgcatggtt tgcgccgctg ccttcactgc
      541 tttctgatct tttggcgtaa aagcccgcaa agcgtgctga acgcgattac cgagctggcc
      601 gagcacatta tccggaatat cagacgggtt ttgcgaaacg aaccagacgc ccacgccttt
      661 tgagcgaata agccttatca cctgctcaat cttatccagc agtacctgcg gggcatcgtt
      721 aaacagcaga tgtgcctcgt cgaagaaaaa caccagtttt ggtttctcca gatcgcccgc
      781 ttccggcaat tgttcataca actctgaaag catccacagc aggctggcgg cgtacagttt
      841 cggcatctga taaagtttct cggcgctgag gatattgata acgcctttac cgttggcatc
      901 ggtgcgcatc cagtctttga tatccagcat cggctcacca aagaagtgtg ctgcgccttg
      961 ctgttccagc gacaacagcc cgcgctggat ggcaccaacc gatgcgctac tgatatttcc
     1021 gtactgattc tggaaggatt tggcgttatc gccgatgtac tgggtaattg cccgcagatc
     1081 tttaaagtcg agcagtaaca gcccctgatc gtcagcaatg cggaagatga tattcagcac
     1141 gccagattgc acatcgttga gattcaacag ccgcgccagc aacagcggcc ccagatccga
     1201 aaccgtcgcc cgcaccggat ggcctttctc gccaaagata tcccacacca ccaccggatt
     1261 ggcatgcggt tgccaatcat tgacgccgat atttttaagc cttgcgagca gtttttccga
     1321 cgccgttcct gcctgcgcga taccggtcag atcgcctttc acatcagcca taaacaccgg
     1381 cacgccgatt tccgacaatg actctgccag tttttgcagc gtaacggttt tacccgtccc
     1441 cgttgcgcca gtaatcagcc cgtgacggtt agccattccc ggcagtaaaa atagttctgt
     1501 gtccggcgtg cgggcaatta acaggggttc gctcatgaga tttcctccag tttcctgcct
     1561 ggagtatagg caacgtccgg caaagagaat acgtgtaatg aagagaaaat aacctaaagc
     1621 gtgttgccgt gataaatctg atacccgagg tcaacagtgt tgtggttgtg atcgttattt
     1681 ttaattttgc tcagcaacat ttgggcagcc gtccgaccaa tatcaaaacg gggagtaatt
     1741 acgctggcga ggcttgggat catttgcctg ccaatctcca gcccatgaaa acccgcaatg
     1801 gaaatttgtt ctggcacggc gaggtttcgc tcgcggcaca gcagaagcgc acccatcgca
     1861 atatcgtcat tggtgcaaaa tacaccgtct aaatccgggt ttgcgctaag ggcatcacgc
     1921 attaactgca ttcctaaatg aatggatgag atggcgcgtg gattcatgcg taatggggaa
     1981 agattatgca gcatcatcgc atcgcaatac ccctgataac gctgttcatc gcgggtgtca
     2041 tctttggaac cgagatacag aattttgtgc ctgactcgct tctccagcat ggtacacacc
     2101 atgtcaaaag cggcctgccg attatcaaaa ccgacctcca tatccagccg ttctccctgt
     2161 acatccatca attccacgac cggaatggtg gctgaacgca gaaatttcac cgtcctgatg
     2221 gtgtgatatt tttccgaaag tataatcccg tcaatgttat aagagagcag gttaatcacc
     2281 gactcttctt cggaatcgcg atcgtaattg taattagcaa taagggtctg ataattatgc
     2341 tcagaggtga cggattctat tcccgcgaga atgtcagcga agagttgatt ttgaaatgac
     2401 ggaatcaaga taccgagggt ataactttgt gcattcaaca acatgccggg agcgcgatta
     2461 gggatataat tgatctcttc cataattttg gcgatgcgct cgcctgtttc ctttgccacc
     2521 tttttcggcg agcggatata acgactcacg gtcatttttg ttacgccagc cagcgtagcg
     2581 atatcctgta aagaaattct gtgattgcgc ataacttctc ccaacgtcgg gggaatcttc
     2641 aatcttcccc cgaaactaca tcacaatatt ttattctttc agtgcaatac ggcgttaatg
     2701 gctaacacgc ccagcaaacc cataatagaa ataagggttt ccatcaccgt ccaggtacgc
     2761 aacgtttcac caaccgtcag attaaaatac cctttaaata gccagaagcc agggtcgttt
     2821 acgtgtgacg cgatcacact gcccgcaccg gttgccagta ccattaaagc gggatcggca
     2881 tgggtaacgt tgattatcgg caacaccaca cccgcggtgg taatggccgc gacggtggca
     2941 gagcccagag cgatacgcaa cagcgccgca acagtccagc acatcaataa cggcgaaagc
     3001 gtagttccgg tcattaagtg tgaaatatac tgcccgacac cgctatctac taatacctgc
     3061 ttaaacgcgc cgccgccagc gataataaac acaatcatcg cgatagcgcc tatagaatcc
     3121 ccaatgatat ccatgatttg ctcgatggtg cgtccattac gtcggcccag tgtgaaaatc
     3181 gcaataacaa tggcaataaa cagcgcaacg gcagggttac cgacaaattc aaagaagagg
     3241 cgcacggtgt tagttttcgg taacgtaatt tcacaaacgg cggcgatagc catcaggatg
     3301 accgggatca cggcagcgaa aatactgttc cagaaggagg gcatctcctc ttcgctaaac
     3361 agatgaggat taaataagcc ttccggtggt gctttctcaa agcgagttag cagtttagaa
     3421 aacagcggtc ctgcgacaat aactgtcgga atggtaatga taaatccata cagtaaagtc
     3481 gttccgagat tagcctcaaa gatagtcgcg atggcagtag gccctggatg tggcggcaga
     3541 aaacagtggg ttacagagag cgctgctacc attggtacgc caacatacaa caggggtaat
     3601 cctgatgatg ctacgatggt aaataccaac ggcaacagca ggacaaaacc cacttcaaaa
     3661 aacatggcga ggcccacaac cagaccggtg atcactagcg cccattgcac gcgtttttta
     3721 ccaaaagtag caatcagcgt agtggcgata cgttgtgccg cacccgtatc agaaatcagc
     3781 ttgcctaaca tggccccgaa accgaggatc attgccagcc cgccgagcgt gctgccgata
     3841 ccattttgta tagagtgcag gacggcctgt gcatccatcc cttcggcaaa tccgacgacg
     3901 gcagctacca gaacgagggc aataaagccg ttaactttaa agccgatcat caggataaga
     3961 agcagcgcga cgcctgccgc aataatgatt aatggcatag tctttatctc ttattagtgc
     4021 cagaaaagca gcgctttcgc gctgcacgta gggggggctg ttaaacagcc actaacatgc
     4081 cgccatcaac aaacaacagg tggccgttta cgaaatcaga ggcttttgaa gaaaggaaca
     4141 ccgcagcacc aatcagttcc tgcggatctc cccagcgtgc ggcgggggtc cgtttgcaca
     4201 accaggcggt gaaggcttcg tcctcaacca gtgctttagt catttctgtt ttgaaatagc
     4261 ccggcgcaat accgttgacc tgaatattgt ggcgcgccag ctcgacgcac atgccgcggg
     4321 tgagcatttt taccgcccct ttcgatgcgg cataaggggt gatggtgtca cgtcccagtt
     4381 cgctttgcat cgagcaaata ttaataactt tacctgcctt gcgttcaacc atgtgacgag
     4441 ttaccgcttg cgataccagg aacacggcgg tctggtttac tgcgatcaca tcattccact
     4501 cttgttcagg gaactcagta aaaggatgac ggcgctggat accggcgtta ttcaccagca
     4561 catcaatggg gccgatgtcc ttttcgatat gttcaacggc ggcatcaatt tcatgtttat
     4621 gagtaacatt aaaaggtgcg gcaacggcct gaataccctc ctggtggagt ttttctacag
     4681 caagttctgc gcgttcggca gtaatatcat taataattat ttgtgcgcca tatttaccca
     4741 ggccggttgc cagtaaaaag ccaatgccct gtgctgaacc ggtaatcaag atattttttc
     4801 ctgccagtga aaatagatcg ttcattgtat tattccttta tttatctctt agaaaacaag
     4861 ctggactttt gctgcctggg ttttatcacc ggcgaaacgt agcgcctctt ccaggtcagt
     4921 gaagggatat tcagcactca gtaaaggcag tggattgata acgccattcg ccagccatga
     4981 cactgcggta ttaaattcgc tggtaaaacg gaaagagcct ctgagtgaaa tctccttacc
     5041 aatcaacgtc atcattggga attctgccat cgcgcctccc atacctacct gcaccattac
     5101 gccgcgtgca cgagtgacct ccagacaggt attcactgat gaaggatgac cggacacttc
     5161 aaagctgaca tcgaaatagc ctttttccgc tttccagtga tccatgtcgt cgttttgtgg
     5221 gtttacgagc acatccgccc ccatctcttt gcccagcgaa agggaacggg gactcacatc
     5281 agcacagaca atttccgcgg cccccagtgt tttcactgca ctgacaatca ggcagccaat
     5341 gggtccaaca ccggaaataa atactcgctt gccctgtaac tcgccggcct gatgtgcggc
     5401 atgaatcgcg acggctaaag gttcggcaaa agccataacc ttttcgtcag ctttggccgg
     5461 ataagggaca cattgcgacg tttcgaccat tttataacgg gtaaaaccac catcaacatg
     5521 agggaaatac atggcactgc caaaaaaacg catatctgta cactgattct cgttatgttc
     5581 aatgcagtat ttgcagtgac cgcacggttt agacggatta atggctaccg tttgcccttc
     5641 atgtaattct gatgagtcgc tatgaataac tttaccgata acttcatgac ctaacaccat
     5701 cggtgccttt atcatgaaat tacctacttt tccttcctga taataatgta aatcggaacc
     5761 gcaaattcca cctcgggtta tttgtactaa tgttccatta ttattccaat ctatcgtctg
     5821 ctcggtaacg gcaacagttt tcttgcccgc aacaacgcag gactgtgttt tcacttgcat
     5881 aaattcctca ctggtcaggt agacacctcg gaagcattta agcggtttta attgtcatta
     5941 tttgtgatga agatcacgtc agaaaattgt tacattacta tgttacgcat aacgtgatgt
     6001 gccttgtaat tcttatcagc agaaataaaa aaacgtgaaa ttattatgcc gccaggcgta
     6061 gtatcgcagc aggtaagatg attcaggaga ttttaaatgg cgggtgaaag ctttattttg
     6121 atgggcgttt cagggagtgg taaaacatta attggtagca aggttgccgc gttattatct
     6181 gctaaattta ttgatggtga cgatcttcat ccagccaaaa atatagataa aatgtcgcag
     6241 ggtattccat tatctgatga agatcgactt ccctggctgg aacgcttaaa tgatgcttca
     6301 tacagtcttt ataaaaagaa tgaaacagga tttattgtct gttcgtcatt aaaaaaacag
     6361 tatcgtgata ttttacgcaa gggtagcccc catgttcatt tcctctggtt agatggcgac
     6421 tatgaaacta ttctcgcgcg aatgcagcgt cgggctgggc attttatgcc ggtagcgtta
     6481 ctaaaaagtc agtttgaggc actggagcgt ccacatgcag atgaacagga tattgtgcgc
     6541 attgatatca accatgatat tgccaatgtc accgaacaat gtcggcaggc tgtgctggcg
     6601 atacgacaaa acagaatatg tgcgaaagag ggcagcgcct cagatcagcg ctgcgaatga
     6661 ttttcaaaaa tcggctttca acaccacgcg gtaacgcgcc ttaccgtcgc gcacatgctg
     6721 gatggcgtcg ttaattttcg acatcgggaa cagttcggta gtcggcgcaa ccttgctgcg
     6781 ggcggcaaag cgcatcagct tacgcaactc ataaggtgta ccggtagcag agcccagaga
     6841 cactgcgatc gcccgcaatc agcgtaaagg ccggaaccgg caacggcgtg agaaccgcac
     6901 cgactgtatg gaaattaccg ccataggtca gcgcctcaaa atagggctgc cagtcgaggc
     6961 tgacgttgac ggtgttgata atgagatcaa actgccccgc cagtgctttc agtgcctgcg
     7021 gatcgcggct attcaccact ttatcggcac ccatcgccag cacttcctgc tctttcgccg
     7081 gattagaact aaaggctgtc acctcgcatc ccattgcgtg cagaagtttt atagcgatat
     7141 gccccagccc gccaatacca attaccccaa cgcggctggt agcagtgata tggtgcatca
     7201 acagtggttt aaagaccgtg ataccgccgc acaacagcgg cccggcggac tcgatatcaa
     7261 tattttctgg cagtggaatc acccattgcc agtccgcacg caacttctcg gcaaagccac
     7321 cgcgattcat aatcgtcggc accgcacctt gctcgcagtt gatctgatta ccgctaatac
     7381 aggcgtcgca gtgaccacag ctacgcgccg tccagccaat cccgacacgc tgaccgacct
     7441 gcaaaccttt atcctgcgcg gcgctcccga gtgccaccac gcgcccaatc acctcatgcc
     7501 cggcaaccag cggatattgt gaaaatcccc attcgttatc gatcatcgac agatcggaat
     7561 ggcagatccc gcagtaatcc acctgcactt caacatcttg tggcttcagc tcaccgggag
     7621 cgtactcata aacttccagt tcgccgcctg cttcttttgc ggcatagctt tttatcatcg
     7681 acattttttg gtccttctct ggtgttgtct gggaagtgta gagcatggca gggcggggtg
     7741 cctggagtgt gacaaaggtt acacatcgct gtatgcaatg ctgaaaattt cagcacttag
     7801 cgaggtgcga gcaagctggc gcttgcatgg tggcgtgcga caggtataat ccacaacgtt
     7861 ttccgcatac ctcttcagtg ccgaagtggc gaaatcggta gacgcagttg attcaaaatc
     7921 aaccgtagaa atacgtgccg gttcgagtcc ggccttcggc accacaccgt atgcaaatcg
     7981 agtcgctcaa gggcggcttt ttttgtgtct gaaatcaccc atatccgtgc agtatatagg
     8041 cctgccagaa aagcagttca gtattcttca ttttgtagtc gatggtccca agtttgctct
     8101 ggctggaagc tgcatcgtcc acattacgtg ggcagggtgg tttcgctatg ctacttgaac
     8161 gttttgacca tgtggctcag gtagctaata tcaaaagacg tcattttgtc tccacgccca
     8221 ccttggtgaa cattcaataa atagtttcgg tcgagagcag ttatgtaggg ctagcagacg
     8281 agatgcgcag acatcttgtt gaagacaatt ttactgacca cctgagaacg ctattctacc
     8341 gaataatatt tattatcttg gcgaataatg atgattacct gtgtaactat ggttttatgc
     8401 tgatgggacc ggcagtacaa tttctgtagt gtactcgcaa tttcagatct ctggcgttta
     8461 ttctggtgca aaggaggtat gtgatgaaaa ctttgctacc atgccattaa atgcgaccaa
     8521 tatcggatta tccatcagtt gcttgttgta gatt
//
LOCUS       DAZKFU020000198         8552 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZKFU020000198 DAZKFU020000000
VERSION     DAZKFU020000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.200"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..1784)
                     /gene="treA"
                     /locus_tag="ACHOQQ_RS18505"
                     /old_locus_tag="pgaptmp_003701"
     CDS             complement(87..1784)
                     /gene="treA"
                     /locus_tag="ACHOQQ_RS18505"
                     /old_locus_tag="pgaptmp_003701"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4471906.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPT
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHVPNGNRSYYLSRSQPPFFAMMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGIENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQREVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSELTTQPVKQKEAEPTP"
     gene            complement(2104..3522)
                     /gene="dhaM"
                     /locus_tag="ACHOQQ_RS18510"
                     /old_locus_tag="pgaptmp_003702"
     CDS             complement(2104..3522)
                     /gene="dhaM"
                     /locus_tag="ACHOQQ_RS18510"
                     /old_locus_tag="pgaptmp_003702"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGU4471907.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSAVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNHQS"
     gene            complement(3533..4165)
                     /gene="dhaL"
                     /locus_tag="ACHOQQ_RS18515"
                     /old_locus_tag="pgaptmp_003703"
     CDS             complement(3533..4165)
                     /gene="dhaL"
                     /locus_tag="ACHOQQ_RS18515"
                     /old_locus_tag="pgaptmp_003703"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HGU4471908.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4176..5246)
                     /gene="dhaK"
                     /locus_tag="ACHOQQ_RS18520"
                     /old_locus_tag="pgaptmp_003704"
     CDS             complement(4176..5246)
                     /gene="dhaK"
                     /locus_tag="ACHOQQ_RS18520"
                     /old_locus_tag="pgaptmp_003704"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HGU4471909.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFVTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            5474..7393
                     /gene="dhaR"
                     /locus_tag="ACHOQQ_RS18525"
                     /old_locus_tag="pgaptmp_003705"
     CDS             5474..7393
                     /gene="dhaR"
                     /locus_tag="ACHOQQ_RS18525"
                     /old_locus_tag="pgaptmp_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HGU4471910.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAVL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(7493..>8552)
                     /locus_tag="ACHOQQ_RS18530"
                     /old_locus_tag="pgaptmp_003706"
     CDS             complement(7493..>8552)
                     /locus_tag="ACHOQQ_RS18530"
                     /old_locus_tag="pgaptmp_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4471911.1"
                     /translation="DVRKNGTNWELYASGTIPEPTPNPEPTPAPAQPPIVNPDPTPNP
                     TPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQSKDGNIWLRSYGGSLDS
                     FASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIGSTHASPDYSGGDGTARSDYMG
                     MYASYMAHNGFYSDLVVKASRQKNSFHVLDSQNNGVNANGTANGLSISLEAGQRFNLT
                     PTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLLGRASMILGYDITAGKSQ
                     LNIYVKTGAIHEFSGDTEYLLNNSREKYSFKGNGWNNGVGVSAQYNKQHTFYLEADYT
                     QGNLFDQKQVNGGYRFSF"
BASE COUNT         2007 a         2135 c         2194 g         2216 t
ORIGIN      
        1 gaccgtagaa tggggggcat ttcgtggcga aaaaagagca atgccaattt tatgcacata
       61 atggcattgc tcgatataca gcaaagttaa ggcgtaggtt ccgcctcttt ttgttttacc
      121 ggctgtgttg ttaattcact gagtggacgc gtcgccggaa cattgtcaca cggttgctct
      181 ttcgggcaga tcaaatccag cattttcagc gtcacgccat tggtccagcc aaagccatcc
      241 tgtaatggat attcgccacc gccgcccccc gttccggtgg tgctgacatc atatttttcc
      301 accagctttt tctcccggtc ataggtgtgc tgaacattgg tcaggaagtg ccagctaatg
      361 tccatcgcca cctctctttg cccgtagttt tgtaatcctt ctgtcgcgac ccactgtaac
      421 ggtgcccagc catttggcgc atcccattgt tgcccacttt tcaccgacgt ggtgttcagg
      481 ccgccgggtt gcagcagatg tgttttcgtc gccgtcgcca ttttgttggc gcgatctttc
      541 gctgccgcat tgacgtacag cgggaacagg gcggccgcgg ttaactgatt gcgcacttta
      601 tgacttttca ggtcgtaatc ggcataccag ccttgttgat cgttccacag gtatttttcg
      661 atcccttttt gacgggcatt tgccagcgtt tcgtactggt ttgccatcgc gttatctccg
      721 gcagctttgc tggcgcgggc gaggattttt tccattttaa acatcaggct gttcagatcg
      781 accggtacga tgctggtggt gcgtaaggta tttaactgct gcgggttgtc catccagcgc
      841 gagctgaaat cccagccaga cgcagcggca gagcgcaggt cgcggtaaat ttcagtggca
      901 ggtcgattcg gattgctttt ggcggtggca atatcttcca cccatgactc tggtcgtggc
      961 gtatcgcgat catcccagta gcggttgaga agggtaccat cctgaagttt tacaacgcgt
     1021 ttttcctgtt gtccggcttg caggttttca ataccgtcca tccagtaagc atattctttt
     1081 tgcatttgcg gcaggtattg cttcaatgca gcatcgcctt catgctgcgc cagtaactct
     1141 accatcatgg caaagaaggg cggctgcgag cggcttaaat agtaactgcg gttgccgttg
     1201 ggaacatgac cgtaagtgtc tatttcatga gcaaaattgg ccaccatatc cgcgacctta
     1261 tcccagtgac cgctttcggc aagtcctaac atggtgaagt aactgtccca gtaatatacc
     1321 tcgcgaaaac gtccgcctgg cacgacataa ggttccggca gcggtaacag agaatcccat
     1381 ttttcggtgt tttcggtaga acgcgttaat accggccaaa gtccgtcaat atgttcgcgc
     1441 agtgactgtc cctctggcgg aacatatttc tcgccctctg tcggcagggt gaaattgacg
     1501 ttaacgaaat ggcgcagatc aaatccgctc tggttttgct gcatccgata atcagcaagg
     1561 atcatcagcg gatcgctgtt cggcacggca tcggcaaagg ttttttggtc cggaaaaagt
     1621 ttggcgtttt gcacatcatt aaacagcggc cctaataaaa tatcaggcgg ctgtggcgtt
     1681 accggtgttt cttctgcctg caccgatagc gcagcgaaac acaaaaagat gcaggctgga
     1741 attaacgcca ttttttgcgg gcgagaaggt gcgggggatt tcatcaatca ttctccttag
     1801 gcgaaaccga ataaaacggt attcaagtat cagaaaacct tagttcagga tcgcactagc
     1861 tgcatgatcg aaatctcatt ctgcgaactg tcaggcattt tagaaaaggg aattaaaggg
     1921 ataaataacg gcgaaataga gtgatcaaat aacgtctgtt tattagccag ccagcggcgt
     1981 ttggttcgcg attagcgaag aaaaatcatc ataaattggt gaaaaatata acaggtagtt
     2041 aaaaaacatt agtgctgagt aaattgccgg atgacatcag aacgatgcca tccgaacggt
     2101 ggtttaactc tgatggttga aacgttgcgt tttaacgtcc agcgttagcg tttcttctgg
     2161 ttgtatcgca tacagtttct caccctgctg gcaaatccag ccaatcccca gttcacgggc
     2221 gatcagggcg ctgtgggata ccggacttcc ggcgctaagg cagatacctt ttacaaccgc
     2281 cggatccagt tgcagtactg cggagggata aatgttctcc gccagtagaa tagttggcga
     2341 gttaaactgc gggagttctt ctttcgtttg ggtcaggtgg accagggtgc gatgcagaag
     2401 atcgtccaca tcaatatagc gagcttgtag atattcatca tccagttgct ggtattgctg
     2461 gctaagttct ttaagaactt gctgccaggc atattctgcc gtgcaatgtt cgtgctgaag
     2521 gagttcgctt gccgccgcca gcagttccgg atcatctaac agtgtatggt gaccagaaaa
     2581 gattgcggca atatcgtcaa gcccgctggc ttctgctttc gctgttaacg tcatcagatc
     2641 taataacgtg aagtcaatgg cctggcgtaa tcgatcttgt tcctcttcca cggtcagggt
     2701 tgatttggcc tgtaccgtac ataaaactgg ttgataataa aatgctttac ccgaaacagg
     2761 cggaacggga cgcagagtag gtggagcaac ttcctccgtt tcaccaaagt tatcttcagc
     2821 cagctgacgg aaagcgatca gtgcctcttc agcttctggc cctttcgcaa tcaggcgcag
     2881 cgtatcgtta tagcgaactt gtagtaacgc aatctggtta atactctccg gtgtgacgca
     2941 tttgccgttt ttctccagca acatatcggc attaaatgtc gataaggtat aaaccagccg
     3001 ggaggctgga cgtacatgca ggccgttacg gttctttatg accaccgcca gagaacgggc
     3061 ttcttcatcg tacggaggac atgtgtcaga gatttcagtg tcggaggacg gtaaacccag
     3121 ttgttcacgt ttggcttcca gcgcatgcat ggcgtcaaag ataactttgt cgatatccgc
     3181 ccccgaggcc gcgctgaccg ttgctgccag tgtaccttcg accaacggtg cagcacacaa
     3241 acgtactttt gcagcgatct cgggagccag caattccagc gcagtttcag cactcaataa
     3301 tgcgctaccc atatccatca tgaccagcac atggtcggca tcggcaacag attcgatggc
     3361 ctccatcact ttgacggcat cggtaccaat gggattttgt ggatcgtcaa ttcccgcggc
     3421 aatggcgatt ttacaactat cactcattaa catctgacgg gctaattcac cgacaccttc
     3481 tcccagtcgg ctgctatgtg aaactatgac caggtttacc atcaccaatt ccttactctt
     3541 ttgcggctaa cgccaacatt tgcatcataa acatcaccga ggttgcgccg ggatcctggt
     3601 gaccaatact gcgttcaccg agataactgg cgcggccttt gcgggcttgc atcgtaatcg
     3661 tactttgtgc agcggattcg gcgatgctac tggcagcttc gagtgccgcc ggaacagaga
     3721 gattttgctc gctggactga cgtaacgatt ccaccaccgg cacccacaca tcacacatgg
     3781 ttttatcgcc aggttcggct ttcccgcgac tgattacgcc gtccgcgcca tcgcggaaca
     3841 tctgataaag ctcttccagc gtcaggcttt gccgtgcctg ggtcgcctgt gcggcgcgta
     3901 taaagaaggt accgaatagc ggaccactgg caccaccgac gctggaaagc agcgtcatac
     3961 cggtattctt gagaatgaaa ccgatatctt tatctgcgat agcagggagt ttttccacca
     4021 ctttgctaaa gcctcggttc atatttagcc cgtggtcagc gtcgccaatc tcgcgatcca
     4081 gtccggtaag atactcgctc tcggtgttga aaatatcgcc acaacgagtg agccagttaa
     4141 caatttgagt tctgctcagt gacattgctt tctccttatt taccccagtt aagggccggg
     4201 gtgtggaccg gggcgtccca gagtgccagc gtttcgtcat caactttcag taaggtgatt
     4261 gagaaaccgg tcatatccag tgaggtgcag tacgcgccaa ttaaattacg ctcgatagtc
     4321 aatcccgctt gctggcaacg tgtggtcagg cggttataga cgccgtacag ctcagaaagt
     4381 ggagttgcgc caagattgtt aaccagcgca atcaccctgt cgccagactg gagcggttgt
     4441 ttggtttgtg gttcttcctg ccagctgcct tgttgataat cccagaaacg caaagtgcga
     4501 tggtatgagc cattttccag cagggtgacg aacatttcat cgacggtttg atcaagggaa
     4561 gagaaggggc gacggtcaat acccggctca ccatgaatgc cgacgccaaa ctccatctca
     4621 ttgtccgcca gggtaaaaga aggtttgccc gcggcaggaa cggtacaggc accgagagcg
     4681 atacctattg agtggccttg attattcaac ttacgcccca gttccgcaca ggcgtccagt
     4741 gagtcgccac gctccgccgc tgcgcctacg agtttttcaa ttaatacggt gttggcaacg
     4801 ccgcgccgcc cggcggtata aagactgtct tttactgcaa cgtcgtcatc aatgaccaca
     4861 gtggtcactt ttacgccgct atcgtgcagt aactcggtcg ctgtttcaaa gttaagaata
     4921 tcgccggtgt aatttttgat aatcaacagt acaccttcgc cgccatcaac ttgcatggcg
     4981 cattcaaaga ttttatcggg cgtcggtgag gtgaaaattt cgcccggaca ggcccccgaa
     5041 agcatcccct gaccgatata accacagtgc atcggctcgt gtccgctgcc gccacccgac
     5101 agcagggcga cttttcctgc aacaggggca tcagctcggg tgacatacac cggatcctga
     5161 tgcagtgtca gcgatggatg cgctttcgcc agtcctgcca gttgttcgtc cagtacgtct
     5221 tgcacatcat tgatcaattt tttcattatt ttgctccagc aattacggta gggcatggat
     5281 gatgttcaac gacacggcga ccactgactg ccgatgaatc cattgtgcat caggcaaggg
     5341 aaaagaaaat tagcccaaat tatgtttcat agtgaaacat atgctttaat gaatgttcca
     5401 tattgaaact tttacgtgta ttaatactta aaattgagag ccggaacacc ttttgtcata
     5461 agggatgcgg gatatgagtg gcgcttttaa caacgatggt cggggcatat ctcccttaat
     5521 tgcaacctcc tgggagcgat gcaataagct gatgaaacgg gagacatgga acgtaccaca
     5581 tcaggcccag ggcgtgacat ttgcttctat ttatcggcgt aagaaagcga tgctgacgct
     5641 cgggcaggct gcgctggaag atgcctggga atatatggca ccgcgagagt gtgcgctgtt
     5701 tattctcgat gaaaccgcct gcattctcag ccgtaatggc gatccgcaaa ccttgcagca
     5761 gctaagtgca cttggattcc atgacggcac gtattgcgcc gagggaatta ttggtacttg
     5821 tgcgctatcg ttagcggcta tctctggtca ggccgtgaaa acgatggccg atcaacattt
     5881 caaacaggca ctctggaact gggccttttg tgcaacgccg ttgtttgaca gcaagggccg
     5941 attgacggga acaatagcgc tggcgtgtcc ggttgagcaa actaccgcag ctgatttgcc
     6001 gttgacgttg gcaatcgccc gcgaggtcgg taatttactg ctgacggaca gtttgctcgc
     6061 tgaaactaac cgtcatttaa atcaacttaa tgccctgtta gaaagtatgg atgatggcgt
     6121 gattagctgg gacgagcagg gtaatttgca atttattaat gcccaggcgg cgcgggtctt
     6181 gcgccttgac gcgacggcaa gtcagggacg ggcaatcact gaactcttaa cgttacccgc
     6241 cgtattgcaa caagcaataa aacaggcaca tccgctcaaa cacgtagaag caacctttga
     6301 aagccagcac cagtttattg atgcggtgat aacccttaaa ccgataatag aaacgcaggg
     6361 aaccagcttt attttgttgc tccatcctgt ggaacagatg cggcagttaa tgaccagtca
     6421 attaggaaaa gtcagccata ccttcgctca tatgccacag gacgatccgc aaacccgccg
     6481 cttgattcat tttggtcgcc aggcggcgcg cagtagcttt cctgtcctgc tttgtggaga
     6541 agaaggcgtg ggcaaggcac tgctaagtca ggcaattcat aatgaaagcg agcgtgctgc
     6601 aggtccttat atcgccgtca attgtgagtt atatggtgat gcggtgctgg cggaagaatt
     6661 tattggcggc gatcgcacgg acaatgaaaa tggccgtctg agtcggctgg aactggcgca
     6721 cggcggcacg ctgtttcttg aaaagattga atatctggcg gtggagttac agtctgcttt
     6781 gcttcaggtt atcaagcagg gtgttatcac gcgactggat gcgcggcgtt taataccaat
     6841 tgatgtcaaa gtgattgcta caacgaccgc ggacctcgca atgctggtgg aacaaaatcg
     6901 ttttagtcgc cagctgtatt acgcgctgca tgcatttgaa attaccatcc cgcctctgcg
     6961 tatgcggcgt ggcagcattc cggctctggt gaataacaaa ttacgcagtc ttgaaaaacg
     7021 cttctctacg cggctgaaaa ttgatgacga tgccctcgct cgcctggttt cctgtgcatg
     7081 gcctggcaac gattttgaac tttacagcgt catcgagaat cttgctctga gtagtgataa
     7141 tgggcgcatt cgcgtcagtg atttgccgga acatctgttt accgagcagg caacagatga
     7201 tgtcagcgcc acacgccttt ccaccagtct gtcatttgcg gaagttgaaa aagaggcaat
     7261 tattaacgca gcccaggtca caggcggtcg cattcaggaa atgtcggctt tacttggaat
     7321 cggccgcact acgctgtggc ggaaaatgaa gcaacatggc attgatgcag ggcagtttaa
     7381 gcgccgggta tgaaagacag aaacgatttc tgatacatca gagtgatcta tatttcattc
     7441 cggcgcacgc taacattttt cagcatcgtt taagggcttg tctatcccgc acttaaaagc
     7501 tgaagcgata tcctccgttg acttgcttct gatcaaagag gttaccctgc gtgtaatccg
     7561 cttcgaggta gaatgtgtgc tgtttgttat actgtgcact gactcccacg ccgttattcc
     7621 agccattacc tttgaagctg tacttctccc gggagttgtt caacagatat tctgtatccc
     7681 ctgaaaactc gtggatagcg ccagtcttca catagatatt cagctggctt ttacctgcgg
     7741 tgatgtcata tcccagtatc atgctggcac gccccagcag cgattcgtag tgattcagat
     7801 gtatattgag gccattactc gccttcatag ccatctcatt ctggtggctg tatgtgagct
     7861 gggtttgcgg ctctatatag aacccataac cagtaggggt caggttgaac ctctgcccgg
     7921 cttccaggga gatgctcaat ccattcgcag tgccgttggc gttaacgccg ttgttctgac
     7981 tgtccagtac gtggaaacta tttttctggc gcgatgcttt tacaacgaga tcgctgtaaa
     8041 aaccgttgtg tgccatgtaa ctggcgtaca ttcccatgta gtctgaacgt gcggtaccgt
     8101 cgccaccgct atagtccggc gatgcatgtg ttgagccaat atacagaccg acatacaacg
     8161 gcattacatc agagagacgt ttatccccac caaactggat accgctgtaa cccatgtcaa
     8221 agccgctcag tttgccactg gcgaaggagt ccaggcttcc cccataactg cgaagccaga
     8281 tattaccgtc tttactctga tttcgcaggt cacccattcg ttgcatcagc gtacggtttt
     8341 caacatagtt taataagtaa ccgacattga gataattgcc gccagcatct gcggtcgtgg
     8401 tgggtttagg tgtgggagtg gggttaggcg ttggatcggg gttgactatg ggaggctgag
     8461 cgggagccgg cgttggttca ggatttggag tcggctccgg aattgtcccg gaagcataaa
     8521 gctcccagtt agtgccattt ttacgcacat cg
//
LOCUS       DAZKFU020000199         8529 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZKFU020000199 DAZKFU020000000
VERSION     DAZKFU020000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.201"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            175..2553
                     /gene="ppsA"
                     /locus_tag="ACHOQQ_RS18535"
                     /old_locus_tag="pgaptmp_003707"
     CDS             175..2553
                     /gene="ppsA"
                     /locus_tag="ACHOQQ_RS18535"
                     /old_locus_tag="pgaptmp_003707"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGU4471912.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVEQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2610..4256)
                     /gene="fadK"
                     /locus_tag="ACHOQQ_RS18540"
                     /old_locus_tag="pgaptmp_003708"
     CDS             complement(2610..4256)
                     /gene="fadK"
                     /locus_tag="ACHOQQ_RS18540"
                     /old_locus_tag="pgaptmp_003708"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HGU4471913.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWQEAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4313..4606)
                     /locus_tag="ACHOQQ_RS18545"
                     /old_locus_tag="pgaptmp_003709"
     CDS             complement(4313..4606)
                     /locus_tag="ACHOQQ_RS18545"
                     /old_locus_tag="pgaptmp_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4471914.1"
                     /translation="MSQNATVNVDVKLGVNKFHVDEGHPHIILAANPDIKEFQKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4603..5892)
                     /locus_tag="ACHOQQ_RS18550"
                     /old_locus_tag="pgaptmp_003710"
     CDS             complement(4603..5892)
                     /locus_tag="ACHOQQ_RS18550"
                     /old_locus_tag="pgaptmp_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4471915.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIHPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMHHFRKIPALMENPRLFSQYPRMIADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5948..6886)
                     /locus_tag="ACHOQQ_RS18555"
                     /old_locus_tag="pgaptmp_003711"
     CDS             complement(5948..6886)
                     /locus_tag="ACHOQQ_RS18555"
                     /old_locus_tag="pgaptmp_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGU4471916.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQVQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6906..7670)
                     /locus_tag="ACHOQQ_RS18560"
                     /old_locus_tag="pgaptmp_003712"
     CDS             complement(6906..7670)
                     /locus_tag="ACHOQQ_RS18560"
                     /old_locus_tag="pgaptmp_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGU4471917.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DNVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            7986..>8529
                     /locus_tag="ACHOQQ_RS18565"
                     /old_locus_tag="pgaptmp_003713"
     CDS             7986..>8529
                     /locus_tag="ACHOQQ_RS18565"
                     /old_locus_tag="pgaptmp_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGU4471918.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVL"
BASE COUNT         2054 a         2177 c         2214 g         2084 t
ORIGIN      
        1 atgtggaaat tgtcgaagag agcagatttg cgcaacgctg ggatcagtct taaaaagtaa
       61 aaaaatatat ttgcttgaac gattcaccgt ttttttcatc cggttaaata tgcaaagata
      121 aatgcgcaga aatgtgtttc tcaaaccgtt catttatcac aaaaggattg ttcgatgtcc
      181 aacaatggct cgtcaccgct ggtgctttgg tataaccaac tcggcatgaa tgatgtagac
      241 agggttgggg gcaaaaatgc ctccctgggt gaaatgatta ctaacctttc cggaatgggt
      301 gtttccgttc cgaatggttt cgccacaacc gccgacgcgt ttaaccagtt tctggaccaa
      361 agcggcgtaa accagcgcat ttatgaactg ctggataaaa cggatattga cgatgttact
      421 cagcttgcga aagcgggcgc gcaaatccgc cagtggatta tcgacactcc cttccagcct
      481 gagctggaaa acgccatccg cgaagcctat gcacagcttt ccgccgatga cgaaaacgcc
      541 tcttttgcgg tgcgctcctc cgccaccgca gaagatatgc cggacgcttc ttttgccggt
      601 cagcaggaaa ccttcctcaa cgttcagggt tttgacgccg ttctcgtggc agtgaaacat
      661 gtatttgctt ctctgtttaa cgatcgcgcc atctcttatc gtgtgcacca gggttacgat
      721 caccgtggtg tggcgctctc cgccggtgtt caacggatgg tgcgctctga cctcgcatca
      781 tctggcgtga tgttctccat tgataccgaa tccggctttg accaggtggt gtttatcact
      841 tccgcatggg gccttggtga gatggtcgtg cagggtgcgg ttaacccgga tgagttttac
      901 gtgcataaac cgacactggc ggcgaatcgc ccggctatcg tgcgccgcac catggggtcg
      961 aaaaaaatcc gcatggttta cgcgccgacc caggagcacg gcaagcaggt taaaatcgaa
     1021 gacgtaccgc aggaacagcg tgacatcttc tcgctgacca acgaagaagt gcaggaactg
     1081 gcaaaacagg ccgtacaaat tgagaaacac tacggtcgcc cgatggatat tgagtgggcg
     1141 aaagatggcc acaccggtaa actgttcatt gtgcaggcgc gtccggaaac cgtgcgctca
     1201 cgcggtcagg tcatggagcg ttatacgctg cattcacagg gtaagattat tgccgaaggc
     1261 cgtgctatcg gtcatcgcat cggtgcgggt ccggtgaaag tcatccatga tatcagcgaa
     1321 atgaaccgca tcgaacctgg tgacgtgctg gtcactgaca tgaccgaccc ggactgggaa
     1381 ccgatcatga agaaagcatc tgccatcgtc accaaccgtg gcggtcgtac ctgtcacgcg
     1441 gcgatcatcg ctcgtgaact gggcattccg gcggtagtgg gctgtggtga tgcaacagaa
     1501 cggatgaaag acggtgagaa cgtcactgtt tcttgtgccg aaggtgatac cggttacgtc
     1561 tatgcggagt tgctggaatt tagcgtgaaa agctccagcg tagaaacgat gccggatctg
     1621 ccgttgaaag tgatgatgaa cgtcggtaac ccggaccgag ctttcgactt cgcctgtctg
     1681 ccgaacgaag gcgtgggact tgcgcgtctg gaatttatca tcaaccgtat gattggcgtc
     1741 cacccacgcg cactgcttga gtttgacgat caggaaccgc agttgcaaaa cgaaatccgc
     1801 gagatgatga aaggttttga ttctccgcgt gaattttacg ttggtcgtct gactgaaggg
     1861 atcgcgacgc tgggtgccgc gttttatccg aagcgcgtca ttgtccgtct ctctgatttt
     1921 aaatcgaacg aatatgccaa cctggtcggt ggtgagcgtt atgagccaga tgaagagaac
     1981 ccgatgctcg gcttccgtgg cgcgggacgc tatgtttccg acagcttccg cgactgtttc
     2041 gcgctggagt gtgaagcagt gaaacgtgtg cgcaacgaca tggggctgac caacgttgag
     2101 atcatgatcc cgttcgtgcg taccgtagag caggcgaaag cggtggttga ggaactggcg
     2161 cgtcaggggc tgaaacgtgg tgagaacggg ctgaaaatca tcatgatgtg tgaaatcccg
     2221 tccaacgcct tgctggcaga gcagttcctc gaatatttcg acggcttctc aattggctca
     2281 aacgacatga cgcagctggc gctcggtctg gaccgtgact ccggcgtggt gtctgaactg
     2341 ttcgatgagc gcaacgatgc ggtgaaagca ctgctgtcga tggcgattcg tgccgcgaag
     2401 aaacagggca aatatgtcgg gatttgcggt cagggtccgt ccgaccacga agattttgcc
     2461 gcatggttga tggaagaggg gatcgatagc ctgtctctga atccggacac cgtggtgcaa
     2521 acctggttaa gcctggctga actgaagaaa taaaataaat acccggcggc gttttgtcgc
     2581 cgggtttatg tgatccccga agatgaaact tattcaatct cttcacagac atcctgagtt
     2641 aatcgccgca taatatcttt ccttaacaaa aacttttgta ttttacctga ggcagtgcgc
     2701 ggtagttttt cgattaccac gatatgttca ggatatttat attttgcgac ccgtttacgg
     2761 ctaaaaaaag ccaccacctc ttccagcgat aatgaatgat gcggcgcttt cagcacgaca
     2821 taagcgcatg aacgttcacc taagcgttca tcgggcattg caaccacaca agcatcgtga
     2881 attttaggat gctgtaataa aatatcttcc acttcacggc tgctaatatt ttcgccgccg
     2941 cggacaataa tatctttctt gcgcccggtt atttttatat agccagcctc atccatgcgg
     3001 cagagatcgc cgctgtaata ccagccttct tcatccaggg cacgggcggt taattcaggt
     3061 tcatcaaaat accccataaa cacattgggg ccacgcgagg cttcttcacc ttcgcaacct
     3121 ggcggtaagg tcttgcgtgc gtcatcgacc actttaatct ctacacctgc ggcagcgtaa
     3181 ccatcggtgt gcataaagcg cgacaaagga tcatcgagat tcaccaccgc atgcggcgaa
     3241 ctttctgtgg aaccataaac acttaataat ttaatgccgc gctgctggca ttcacgcgcc
     3301 acttttttgg ggattgtggt accgccgcaa agaaagaagc gcagcgctga aaggtctgcg
     3361 ggttgtttct ctagtaaatt caaaagatca tagacaaacg gcgttgcgcc gagcatacag
     3421 gtgcaacgct gctgctcaag cagcgcgaga cacgcatcag gagtgaaaat atctaacaac
     3481 acgctgcgag cgccaattaa gaatggtgcc gttacgccat gcagaaagcc cgttgcgtgg
     3541 ccgagtggcg caggcatcat aaagacatcc tgccaggtca gattcagccg cgcgcaataa
     3601 gcccgctcac tggcgagaat attgttatgc gttagcatca cgccctttgg cagaccctcg
     3661 gttccggagg taaacagcac cgcggctaat tcatcgccgt ggaccgttat cgccgtggtc
     3721 agtggggtat tgtcggcgat aatctgactt aatgagaggg aagaggtggc gggagccagt
     3781 ttgtccacgc cgacaatttg ttgtagttgt ggaagctgat tttgcagcgg caggattaaa
     3841 tctaccggac gcgtttgttt aaacaacgtc ggtgcaaaga acatttttgc ctgacactta
     3901 ttgagcaccc acaccagttc tgcttcctgc caggaaggca acagcggcac ggaaaccgca
     3961 ccgattttca ggcaggcaag atagataacg gtaaattcac accagcctgg cagttgaaat
     4021 gcgatgcgat cgcctgattc aatacccttc gctaacatcc agttagccag acagctcgcg
     4081 gcgtgatcga gcgcgctata ggtgtacgat gctccatgat tatcgaccac ggcaattttg
     4141 tctggcatcg cacgagcggt ctgctgccag taatcggcca gcgaagcatc gccccatagc
     4201 ccttgctgac gatacgccgc acgacgttgt tcgttaaacg ttaatgtcac tttcattttg
     4261 gactctcgaa acagaacatc aggcccgaga tgcgggcctg tgggatgcat gattagccgt
     4321 agcgaaaatc aataccgaag gtgcctgcgg gatattgcca ctgttcgaga atagtgttac
     4381 cgcacaacac ccgacaggtg ccgcactcca gacaaccggc agaatcaaaa tgaatgttgc
     4441 cggcgtcatc ctgcttataa agtccggcag ggcaggcttt cattaatttt tgaaattctt
     4501 tgatgtcggg atttgccgcc agaataatat gtggatggcc ctcatcaaca tggaatttat
     4561 tgacgcctaa tttgacatca acgttaaccg tagcgttctg gctcatagcg cggttgctcc
     4621 cttaatgcca tctttcagca gattgatcag cccgattttc ttcgcgtgtc ccatgatcat
     4681 tttgcgtacc ggctggtttg gtttgccgtc aatggtgaac atatcgttca tgatgtcggc
     4741 gatcatgcgc ggatactgac taaacaggcg cggattttcc atcagcgccg ggatcttgcg
     4801 aaaatggtgc atatcgcgca taacacaact ttgttccagc tcacgtttgt attgcgcaag
     4861 actgctggcg gagaaatccg cgcgttcttt ggcggcgatc accgttgtgg cggcagcctg
     4921 agccgatgca atggctaaat ccatgccgcg gaccgtaaaa cccaaattca ggcagaagcc
     4981 tgcggcgtca ccaacgatca tcacgccctc gttaaccagt tgcggcacca ttgccagacc
     5041 gccttccggc accatatgcg cggaatattc aagcagtttg ccgccgctaa tcagcgggtg
     5101 aatggcgggg tgttgtttaa aatcttccag catttgcggc acgctttttt gcgcatgggc
     5161 gatatcaccc aatccacaaa ccagccccaa cgatatggaa tccttattgg tatagaggaa
     5221 tccgccaccc atcaggccgt cagaagggga accggcaaac agccaggcgg caccttcctc
     5281 gcccgtaata ttaaagcgat cgttgatctg ttctggtgtg aggccaataa cctctttaac
     5341 accaacagcg taatgatgcg gatcggaagc gggaaccatt cccagcgaac ggccaagcat
     5401 cgagttaacg ccatcagcca gaatcaccac attcgcttcg agaatatcat ccccagcctg
     5461 gacgccagtg accttgtttc cttcacgaac cagcgcatcg acgcgaactc ccgggataaa
     5521 ctgtgcgccc gcctgctcgg cttgttccat caaccacggg tccagacgat tacgcaatac
     5581 ggtataagat gcgtgttgcg gaacatctgg ttgctcgcgg tgaaaatcga gggtaacggc
     5641 gctctcttcg gttaagaagg agattttctc gcgtgtgacc ttgcgttcta ccggcgctga
     5701 ggctgcaaag cctggaatga ttgcttcaag tgtgtgggca taaagacgcc cgccggtcat
     5761 gtttttacat ccggcactgt cgccgcgttc tatcaccagg acatccagcc cggctcgtgc
     5821 catgaccagt gcggcaacgc taccagcaac gcccgcaccg accacaatgg catcaaattt
     5881 gtcatccgac atgcgttatg ctcccttccc gctgcgttta cagcgggaga tgtggttgaa
     5941 aggggaatta gcggctcaac tggctaatca gggcagggac gactttgtag atatcgccca
     6001 ccagaccgta gtcggcatag ttgaagattg gcgcattttt atctttattg atggcgacaa
     6061 tcacttttgc gccgttgccg ccaaccatat gttggatctg cccggagatc cccagcgtca
     6121 ggtagagatc ggatttcagc aacacgccgg agacaccgat ataacgttca cgctccatcc
     6181 agttctcgcc ttcggcaatt ggacgtgaac agccgacttc agcattcagc accgccgcca
     6241 gttcgtggac cattttcagg tcatcctgcg ctgccagacc acgaccgacg ccaaccacac
     6301 gttttgcttt gctcaggtcc acgctgcttg ccgctttcgc acggcgctcg cgacagagaa
     6361 tttcatgacg cggagcaaca taaggtaccg tttctgtcgg gcactgatga gaggtatcac
     6421 tggtgcacgg ttcctgaaca ccgggtgcaa gggtaatgat cgctagcgag ctgttgatct
     6481 tttcctgagc gaacgctaac ccgccataca tccggtgttc ggcgcaaatg tgaccatcga
     6541 caatatccac cgccgtggca tcgttcacca gcgccgcatt cagttgcaca cttaaccgtg
     6601 ctgccagagc tttaccacgt ttcgtcgcgg ccagtagcaa catagcgggg tgtttatctt
     6661 tcagcagagc agcaatgctt tcggcgtaat tttcagtgcg ttgcagcgcg tcgttttgct
     6721 caagaacata aatacatttt ggaccataag gcataactgc ctgcacctgg tcggtatttt
     6781 gtacaatggc atacacctgt tggccccatt gctgagcgcc gccaaacagt tcagcataac
     6841 gttcaggatt atcgctaaag acccagacgc tgtttaattg actcatcgtt tcttctccgt
     6901 aggcttcagt tcagggcttt cttcagatgt tccgccagct cggcaatggc ctccggcgaa
     6961 tcgttatcga gaatgatgtg cttacgttct gtttgcggcg gtacgcgaat gccggtcagt
     7021 tcagcaagtg gtgcgctctg gctccagcca atatcacttg cctgccactg atttaccggt
     7081 tttttacccg cgccgagaat ggctttcatc gaaggaatgc gtggcacgtt gatatcggag
     7141 gtgacgcaga gcacggctgg aacagagagt tcaataactt caacgttatc ttcaagcgtg
     7201 cgttcaatca ccagtgtatt gccctgacgc tgaatagcac tcactgcatt aatcaccgga
     7261 agttgcagaa tttctccgac cagcaagcca acctgctggg cataaaggtc gccggaacct
     7321 tcaccaaaga tcagtaaatc gaagccgatc ttttcaactg ctgccgccag cgcttttgcg
     7381 gtatcgagag gcagtgcatg ttcaagttgc gcatcctgca ccaaatacag gctgtgcggc
     7441 ccgcgggata acacgtcttt gcgcactttc gagttctgca acaatgagcc gccaacggtc
     7501 agcgcggcta tctcatcgtc atctgttgca agctggcttg cagcttcaat ggcattgaga
     7561 tcgaactggc tgattttggc gtcggcattg tcgaaattca gggtgtattc tggagtgaca
     7621 acaatgtcct gttcttcagg caccagctta aagcaggtta ttattttcat gatttctcct
     7681 gtgagtcgca tacggaacca tctccgatgg aaggtaaaaa tggcctgaaa atagtgtgcg
     7741 atagcggtgc ggggttttcg acggagcaag ctgttttcct tgccaacaat cttgcaaaaa
     7801 aaatgcgatc gctaaaacaa aagtgcaatt tgcggcaata tagttattcc atgaccgtac
     7861 aatcttgcat tcagtgtaaa taatcttgtt tagctctcgc taaacgcggt tgtataaccg
     7921 caatacacgc cgtattttat ccggcatata ttgtttttgc ttgctcatgg aatagggagg
     7981 ttgtcatgta tcaacgctgt tttgataatg ccagtgaaac gctgtttgtc gccggtaaaa
     8041 cgccacggct ttcacgtttt gcatttagcg atgatccaaa atgggagtct ggacatcacg
     8101 ttcatgacaa tgaaaccgag ctgatttacg tcaagaaagg ggtggcaagg tttaccatcg
     8161 attcttcgtt atatgtcgcg catgcagatg atatagtggt gatagaacgc ggcaggctgc
     8221 atgcggtggc ctctgacgtt aacgatccgg caacgacgtg tacctgtgcg ctgtacggct
     8281 ttcagtttca gggggctgag gaaaatcatc tactgcaacc gcattcttgt ccggtaattg
     8341 ccgcggggca gggaaaagaa gtcattaaaa ccttatttaa tgagctaagt gtgattttgc
     8401 cgcaaagtaa aaatagccaa acatcttcgt tatgggatgc atttgcctat acattagcaa
     8461 ttctttacta cgaaaacttt aaaaatgctt atcgttcgga gcaggggtat attaaaaaag
     8521 atgttctga
//
LOCUS       DAZKFU020000200         8527 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZKFU020000200 DAZKFU020000000
VERSION     DAZKFU020000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.202"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            79..513
                     /gene="slyA"
                     /locus_tag="ACHOQQ_RS18570"
                     /old_locus_tag="pgaptmp_003714"
     CDS             79..513
                     /gene="slyA"
                     /locus_tag="ACHOQQ_RS18570"
                     /old_locus_tag="pgaptmp_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGU4471919.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(560..1027)
                     /gene="slyB"
                     /locus_tag="ACHOQQ_RS18575"
                     /old_locus_tag="pgaptmp_003715"
     CDS             complement(560..1027)
                     /gene="slyB"
                     /locus_tag="ACHOQQ_RS18575"
                     /old_locus_tag="pgaptmp_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGU4471920.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1301..2410
                     /gene="anmK"
                     /locus_tag="ACHOQQ_RS18580"
                     /old_locus_tag="pgaptmp_003716"
     CDS             1301..2410
                     /gene="anmK"
                     /locus_tag="ACHOQQ_RS18580"
                     /old_locus_tag="pgaptmp_003716"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGU4471921.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            2508..2837
                     /gene="mliC"
                     /locus_tag="ACHOQQ_RS18585"
                     /old_locus_tag="pgaptmp_003717"
     CDS             2508..2837
                     /gene="mliC"
                     /locus_tag="ACHOQQ_RS18585"
                     /old_locus_tag="pgaptmp_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGU4471922.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNSRR"
     gene            2896..3552
                     /gene="pdxH"
                     /locus_tag="ACHOQQ_RS18590"
                     /old_locus_tag="pgaptmp_003718"
     CDS             2896..3552
                     /gene="pdxH"
                     /locus_tag="ACHOQQ_RS18590"
                     /old_locus_tag="pgaptmp_003718"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGU4471923.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3681..4955
                     /gene="tyrS"
                     /locus_tag="ACHOQQ_RS18595"
                     /old_locus_tag="pgaptmp_003719"
     CDS             3681..4955
                     /gene="tyrS"
                     /locus_tag="ACHOQQ_RS18595"
                     /old_locus_tag="pgaptmp_003719"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGU4471924.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            5014..5877
                     /gene="pdxY"
                     /locus_tag="ACHOQQ_RS18600"
                     /old_locus_tag="pgaptmp_003720"
     CDS             5014..5877
                     /gene="pdxY"
                     /locus_tag="ACHOQQ_RS18600"
                     /old_locus_tag="pgaptmp_003720"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HGU4471925.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5921..6526)
                     /gene="gstA"
                     /locus_tag="ACHOQQ_RS18605"
                     /old_locus_tag="pgaptmp_003721"
     CDS             complement(5921..6526)
                     /gene="gstA"
                     /locus_tag="ACHOQQ_RS18605"
                     /old_locus_tag="pgaptmp_003721"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGU4471926.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(6632..8134)
                     /gene="dtpA"
                     /locus_tag="ACHOQQ_RS18610"
                     /old_locus_tag="pgaptmp_003722"
     CDS             complement(6632..8134)
                     /gene="dtpA"
                     /locus_tag="ACHOQQ_RS18610"
                     /old_locus_tag="pgaptmp_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGU4471927.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         2215 a         2095 c         2162 g         2055 t
ORIGIN      
        1 tgttttagca atacaattgc tgcacactat tctaaaagcc gcataatatc ttagcaagct
       61 aattataagg agatgaaatt ggaatcgcca ctaggttctg atctggcacg gttggtgcgc
      121 atatggcgtg ctctgataga ccatcgcctg aaaccgctgg agttaacaca aacccattgg
      181 gttacgttac acaatatcca tcagttacct ccagaccagt cgcaaattca actggcaaaa
      241 gcgattggca tcgagcagcc atcactggtc cgtactctgg accaactgga agaaaaaggg
      301 ttaatttcgc gtcaaacttg tgccagcgat cgtcgggcta aacgtattaa actgacggaa
      361 aaggcagagc cgctgatcag cgaaatggaa gctgttatta acaaaacccg cgcggaaatc
      421 ttacatggca tctccgcaga ggaactggaa caactgatta cgctcatcgc aaaacttgag
      481 cataatatca ttgagttaca ggccaaaggg tgaaatgaag ggggcgtgtg gtcagtaacc
      541 tgaccacacg caaacttatt tagcgcggag aaacggtcac ctgactgcca ttgctggcca
      601 gtacgacacg ttggcccgga gagaaacgag tgttgccttg tttctgtacc accatgatgg
      661 tattaccatc gtctttacga atttccagct cgacaccttg cgttttgttc attgcactct
      721 gtacgccctg accagctacg ccacctgcaa cagcgcctgc tgcagtagcc agagaacgcc
      781 cggttccgcc accaacagta tttcccagga aaccaccaag aacagcaccg ccaattgcac
      841 cgataacgtt ggaatcatca ccgccctgaa tctgtaccgg acgtacgtta acgatggttc
      901 catagctgac attctgtact tgtttcgctt cagaagcggt ataaacatcc cctgacaggg
      961 tgtcgttatt aacacaaccg acaagagaca gacctaccat tgaaacaacc aatacgcgtt
     1021 taatcattga aaaatctcct gttcaccatg aaacgccacg caagcatccc tcatggtcaa
     1081 agtatatggc atatgtgaat ccatggttac atgttctacc aaatatgagc aaatcataat
     1141 gaaagtagga tcaaccaatt ataaacaaaa cattcatgat gattattgat ttgaagataa
     1201 tgttttacta ccaacggtaa aaatcggtca ttccgcatgg tattcacgaa aagtttatgt
     1261 ttgagtggtg cgcaaacgtc agattgaaaa aggatgaact atgaaatcgg gccgctttat
     1321 tggcgttatg tcaggcacca gccttgatgg tgttgatgtt gtgttggcga caattgatga
     1381 acaccgggtc gcacagctgg caagtttgag ctggccgatc ccggtgtctc tgaaacaggc
     1441 tgtactggat atttgccagg gccagcagct tacactttcg cagtttggac agcttgatac
     1501 tcaactcggg cgactttttg ctgatgcggt caatgccttg cttaaggaac aaaacctgca
     1561 ggcgagagat atagttgcga tcggctgtca cggtcaaacc gtctggcatg aaccgacggg
     1621 cgtggcacca cacactttac agattggcga taacaatcaa attgtggcac gcaccggaat
     1681 tacggttgtc ggtgattttc gccgtcgcga tattgccttg ggaggacaag gcgcaccgct
     1741 ggtacctgcg ttccatcatg ccttgctggc tcacccaacc gagcgacgaa tggtgctcaa
     1801 tattggcggc atcgccaatc tgtcactgct cattcctggg cagccggttg gcggctacga
     1861 taccggtcct ggtaacatgc tgatggatgc ctggatctgg cgtcaggccg gtaaacctta
     1921 cgataaagat gccgagtggg cacgggcggg taaagttatt ctcccactgc tgcaaaatat
     1981 gctcagcgac ccatatttct cgcaacctgc accgaaaagc accggacgcg aatactttaa
     2041 ctacggctgg ctggagcgcc atttacgcca ttttccgggt gttgatcccc gtgatgttca
     2101 ggccacactg gcagaactca ccgccgtgac catttctgaa caagttttgt tgagcggtgg
     2161 ctgcgaacga ttgatggtat gtggtggagg tagtcgtaat ccgctactca tggcgcgtct
     2221 ggcggcatta ctgccaggca cagaagtcac caccaccgat gccgttggca ttagtggcga
     2281 tgacatggaa gcattggctt tcgcctggct tgcctggcgg acgctggcgg gattaccagg
     2341 aaatctgcct tccgtcactg gcgcaagcca ggagacggta ctgggggcta ttttcccggc
     2401 taacccgtga tatcagagtt aactgaattt ttcttaccgc gtctgccgtt acacttggaa
     2461 cgttagggag ggcgtattgc cctccagacc aggaaagtct tcgggaaatg tctatgaaaa
     2521 aactgttact gatctgtttg ccggtgcttc tcaccggctg tagcgcgttc aatcaactgg
     2581 ttgagcgcat gcaaaccgat acgctggaat accagtgtga tgaaaaaccg ttgacggtca
     2641 aactgaataa tccgcgccag gaagtcagtt ttgtttacga taatcaacta ctgcatctca
     2701 aacagggcat ttcagcctct ggtgcacgtt acactgacgg aatctatgtt ttctggtcga
     2761 aaggcgagga agcgactgtc tataaacgcg accgcatcgt cttgaataac tgtcagttac
     2821 aaaattcacg gcgttgagat ttttccaggg gcggcgcaca atagcgtcac ccactgacaa
     2881 tccgtaaaga aaaccatgtc tgataacgac gaattgcagc aaatcgcgca tctgcgccgt
     2941 gaatacacca aaggcgggtt acgccgccgc gatcttcccg ccgatccatt aacccttttt
     3001 gaacgttggc tctctcaggc ttgtgaagcc aaactggcgg accctaccgc gatggtggtc
     3061 gctaccgtgg atgaacatgg tcagccttat cagcgcatcg ttttactcaa acattacgac
     3121 gaaaaaggca tggtgtttta caccaacctc ggtagccgta aagcacatca aattgaaaat
     3181 aatccgcgcg ttagcctgct gttcccgtgg catacccttg aacgccaggt gatggtgatc
     3241 ggtaaagcag aacgactttc gactctcgaa gtgatgaaat attttcatag ccgcccgcgt
     3301 gatagccaga ttggtgcatg ggtttcgaag cagtccagtc gcatttctgc ccgcggtatc
     3361 cttgaaagta aattcctgga gctgaagcag aagtttcaac agggcgaagt gccattgccg
     3421 agcttttggg gcggttttcg cgtcagcctt gaacagattg agttctggca gggcggtgag
     3481 catcgcctgc atgaccgctt tttgtaccag cgtgaaaatg atgcgtggaa gattgatcgt
     3541 cttgcaccct gaaaagatgc aaaaatcttg ctttaatcgc tggtactcct gattctggca
     3601 ctttattcta tgtctctttc gcatctggcg aaaagtcgtg taccggcaaa ggtgcagtcg
     3661 ttatatacat ggagattttg atggcaagca gtaacttgat taaacaattg caagagcggg
     3721 ggctggtagc ccaggtgacg gacgaggaag cgttagcaga gcgactggcg caaggcccga
     3781 tcgcgctcta ttgcggcttc gatcctaccg ctgacagctt gcatttgggg catcttgttc
     3841 cattgttatg cctgaaacgc ttccagcagg cgggccacaa gccggttgcg ctggtaggcg
     3901 gcgcgacggg tctgattggc gacccgagct tcaaagctgc cgagcgtaag ctgaacaccg
     3961 aagaaactgt tcaggagtgg gtggacaaaa tccgcaagca ggttgccccg ttcctcgatt
     4021 tcgactgtgg agaaaactct gctatcgcgg cgaacaacta tgactggttc ggcaatatga
     4081 atgtgctgac cttcctgcgc gatattggca aacacttctc cgttaaccag atgatcaaca
     4141 aagaagcggt taagcagcgt ctcaaccgtg aagatcaggg gatttcgttc actgagtttt
     4201 cctacaacct gttgcagggt tatgacttcg cctgtctgaa caaacagtac ggtgtggtgc
     4261 tgcaaattgg tggttctgac cagtggggta acatcacttc tggtatcgac ctgacccgtc
     4321 gtctgcatca gaatcaggtg tttggcctga ccgttccgct gatcaccaaa gcagatggca
     4381 ccaaatttgg taaaactgaa ggcggcgcag tctggttgga tccgaagaaa accagcccgt
     4441 acaaattcta ccagttctgg atcaacactg cggatgccga cgtttaccgc ttcctgaagt
     4501 tcttcacctt tatgagcatt gaagagatca acgccctgga agaagaagat aaaaacagcg
     4561 gtaaagcacc gcgcgcccag tatgtactgg cggagcaggt gacccgtctg gttcacggtg
     4621 aagaaggttt gcaggcggca aaacgtatta ccgaatgcct gttcagcggt tccttgagtg
     4681 cgctgagtga agcggacttc gaacagctgg cgcaggacgg cgtaccgatg gttgagatgg
     4741 aaaagggcgc agacctgatg caggcgctgg tcgattctga actgcaacct tcccgtggtc
     4801 aggcgcgtaa aactatcgcc tccaatgcca tcaccattaa cggtgaaaaa cagtccgatc
     4861 ctgaatactt ctttaaagaa gaagatcgtc tgtttggtcg ttttacctta ttgcgtcgcg
     4921 gcaaaaagaa ttactgtctg atttgctgga aataatgcat taagtggaaa gggggagtga
     4981 gaaatcactc cccctggttt ttatacaggg aacatgatga aaaatattct cgctatccag
     5041 tctcacgttg tttatggtca tgcgggtaac agtgcggcag agtttccgat gcgccgcctg
     5101 ggcgcgaacg tctggccgct gaacaccgtt caattttcta atcacaccca atacggcaaa
     5161 tggactggct gcgtgatgcc gcccagccat ttaaccgaaa ttgtgcaagg cattgccgcc
     5221 attgataaat tacacacctg tgatgccgta ttaagtggct atctgggatc ggcggagcag
     5281 ggtgaacata tcctcggtat cgtccgtcag gtgaaagccg cgaatccgca ggcgaaatat
     5341 ttttgcgatc cggtaatggg tcatccggaa aaaggctgta tcgttgcacc gggtgtcgca
     5401 gagtttcatg tgcggcacgg tttgcctgcc agcgatatca ttgcgccgaa tcttgttgag
     5461 ctggaaatac tctgtgagca tgcggtaaat aacgtcgaag aagcggttct ggcagcgcgc
     5521 gaactcattg cgcaagggcc acaaattgtg ttggttaaac acctggcgcg agctggctac
     5581 agccgtgacc gttttgaaat gctgctggtc accgccgatg aagcctggca tatcagccgt
     5641 ccactggtgg attttggtat gcgccagccg gtaggtgttg gtgatgtgac gagcggttta
     5701 ctgctggtga aactgcttca gggggcaacg ctgcaggagg ccctggaaca tgtgaccgct
     5761 gcagtatacg aaatcatggt gaccaccaaa gcaatgcagg aatatgagtt gcaagtggtt
     5821 gctgctcagg atcgtattgc caaaccagaa cattacttca gcgcaacaaa gctctgaaat
     5881 atctcatttc ggcccacaac gaactgtggg ccgacggcta ttactttaag ccttccgctg
     5941 acagcgcgtc ttgtacttcc ggacgttcag ccatacgttg cataaatgcc gcaatgtgct
     6001 ctaacccttc cagattcagt ttcaccgcgt atgcccagcg cagtaccgta aacagatagg
     6061 catcagcaat tgtaaatctt tgcccgcaga tccagtgctc atccttcagt gcctcgttca
     6121 catattgcag cttcttctcc agctgcgcgc gaactgtcgg tttgtactct tccggtgtat
     6181 cagggcgaaa cagaggtgtg aaacctttat gcagctcggt ggcgatgtaa ttcagccatt
     6241 cgatggtttt atagcgggaa atactgttta ccggtgccag caactggcgg tcggggacgc
     6301 tgtcggcaag atactgcata atcgctacac cttccgtcag caaagtaccg tcatccagca
     6361 gcaatgcagg tacctgtccc ttagggttaa cggcaaagta atcgtcaccg ttttcgagac
     6421 gttttttcat taaatccaca ctgacgagcg taaaatcctt tccgctctca cgcagggtga
     6481 tatgggaagc gagagagcag gcgcccggtt tgtagaacaa tttcatcggt aactcctttt
     6541 gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa aaaaagccgc taacgaaaag
     6601 ttagcggctt gtaaaagagt ttccctgaat tttacgctac agcagctttc gccgctttgt
     6661 cagcagcgtc atcctgcgtc atgcggtgca gtttcggcgc ggtaaacagc atcagtactg
     6721 caataacggc agtagcgaca ccaatctgca agaatacgcg accatagact tccagtgaca
     6781 tcagcggatc ggtaacgtta tccggcacag ccatcatacc cgcaacataa ccaccaatca
     6841 ggtttgcacc ggcagtggtc aggaaccagc tacccataat gaagcccatc agacgctgcg
     6901 gaacgagttg agcaaccatt gccagaccca gaccagagat cataagttcc ccgatactct
     6961 gcaggccata gcttgcgacc agccagctta cagacacgat acctgcgtca gacgcgaatt
     7021 ttgcacccag cggcagaatc aggaacgcac cagaacacat caccataccg attgcaaact
     7081 tagttggcat cggcagggta tcgcccatct tgttatagat agcggccaga atcgggctac
     7141 caatgatgat ccagaacggg ttcagtgcct gatactgctc aggttctacg gccaggccca
     7201 gaatggtgtg ttcaacgtta cgaatcgcaa agaagttcag tgacgttggc atctggctgt
     7261 acagcacgaa gaagataatg gcttcgagca tcaggatgaa ggcaacgatc attttacgac
     7321 gcgcagcacc tttcatcgcg aaggcttctt taccgaagat aaccacgata ccaaaggcaa
     7381 caacgcccag cgccatacgc gcaacttcct gattgtgcag cagccaggtg gcgatagcga
     7441 tcagtgccac aacaccaata atggtcagca gcaggttacg gtagttgata ggctcgaagt
     7501 ctggttttga accgtactgt ttaacccagc gttggcagaa ggcgaagtta acaatagtga
     7561 tcagcaggcc tacaacgctc aacgcaaacg caacactcca gccgtatttc gcggccagcc
     7621 acggggtagc gatcatagag aagaaagagc cgatgttgac ggacatgtag tacatggtga
     7681 atgcaccgtc cagacgcggg tcgtttttct catagcatgt agaaagcaga gaagacgggt
     7741 tagctttaaa caggccgtta ccgaccgcaa tagccgccat acccatataa acgataccgg
     7801 cgtcgtgacc agaccaggca accagagcat aaccaatcgc cagcacaata gcgccgagca
     7861 taattacgcg tttagtaccc agtaccttgt cacctaacca gccgccgata gcgaccagac
     7921 cataaaccag ggcactaaag gaagagaaaa gggtgattga atccgcttca gacataccca
     7981 gttgtttaac caggtaaaca gccataattc cttgtaggcc gtaataacca aaacgttccc
     8041 ataactcaat cgagaagatg agatagaacg ccttcggttg tttgaaagcg ttcaaactga
     8101 cgctttcagt tggtttttgg tttgcagtgg acacttttac ctcttttttt acatcccata
     8161 ttaacggggg tgctttctcg cgttatgcct aaaaacatac gcgcaattgt tatagtggga
     8221 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     8281 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     8341 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttga
     8401 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     8461 agccagtgta atgttttgta ggtcaatatt tgttgcgtgt gtaaatttaa atgattttaa
     8521 cctgatt
//
LOCUS       DAZKFU020000201         8408 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZKFU020000201 DAZKFU020000000
VERSION     DAZKFU020000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.203"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(150..416)
                     /locus_tag="ACHOQQ_RS18615"
                     /old_locus_tag="pgaptmp_003723"
     CDS             complement(150..416)
                     /locus_tag="ACHOQQ_RS18615"
                     /old_locus_tag="pgaptmp_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGU4471928.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(413..1180)
                     /gene="yjiL"
                     /locus_tag="ACHOQQ_RS18620"
                     /old_locus_tag="pgaptmp_003724"
     CDS             complement(413..1180)
                     /gene="yjiL"
                     /locus_tag="ACHOQQ_RS18620"
                     /old_locus_tag="pgaptmp_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGU4471929.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            complement(1190..2341)
                     /gene="yjiM"
                     /locus_tag="ACHOQQ_RS18625"
                     /old_locus_tag="pgaptmp_003725"
     CDS             complement(1190..2341)
                     /gene="yjiM"
                     /locus_tag="ACHOQQ_RS18625"
                     /old_locus_tag="pgaptmp_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGU4471930.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQREEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2457..3737)
                     /gene="yjiN"
                     /locus_tag="ACHOQQ_RS18630"
                     /old_locus_tag="pgaptmp_003726"
     CDS             complement(2457..3737)
                     /gene="yjiN"
                     /locus_tag="ACHOQQ_RS18630"
                     /old_locus_tag="pgaptmp_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGU4471931.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3778..5010)
                     /gene="mdtM"
                     /locus_tag="ACHOQQ_RS18635"
                     /old_locus_tag="pgaptmp_003727"
     CDS             complement(3778..5010)
                     /gene="mdtM"
                     /locus_tag="ACHOQQ_RS18635"
                     /old_locus_tag="pgaptmp_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGU4471932.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSMLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            5477..6421
                     /locus_tag="ACHOQQ_RS18640"
                     /old_locus_tag="pgaptmp_003728"
     CDS             5477..6421
                     /locus_tag="ACHOQQ_RS18640"
                     /old_locus_tag="pgaptmp_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4471933.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQQRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            complement(6663..8075)
                     /gene="yjiR"
                     /locus_tag="ACHOQQ_RS18645"
                     /old_locus_tag="pgaptmp_003729"
     CDS             complement(6663..8075)
                     /gene="yjiR"
                     /locus_tag="ACHOQQ_RS18645"
                     /old_locus_tag="pgaptmp_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4471934.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            8252..>8408
                     /locus_tag="ACHOQQ_RS18650"
                     /old_locus_tag="pgaptmp_003730"
     CDS             8252..>8408
                     /locus_tag="ACHOQQ_RS18650"
                     /old_locus_tag="pgaptmp_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGU4471935.1"
                     /translation="MEFHKNKAKTPFIRLVLLWHAMRRWRRQMQTRRVLQQMSDERLR
                     DIGLRRED"
BASE COUNT         2182 a         2240 c         2128 g         1858 t
ORIGIN      
        1 ttatatttta gacgtaaaat agtaaggtgc tatcaaaaat cgctcatttt gtggatggga
       61 aatatttcac ggcaggcgag atatttacta caacttcctt catgcaggat tatgcgcagc
      121 caattatccg gttggtatat ttaaagatcc tattgcctgg ttgacgcttc tgcatcgtaa
      181 tgcaccacgc actgccagtc gctgccctgc tggcaatata tcgcttcggt ttgcccaggg
      241 atcacccatt gtatttcctc gccgggggga caggtcagcc aaatgcataa cccgcagccg
      301 ccgcgtaaat cacggggaat atcgctgacg cgaaaagtca tgcccgctgc ctgcaacgcc
      361 ttgcgggttt gtatgacgcc gaccgtggag tgaaataaaa ataaaaactc tttcatcggc
      421 gtgtcctcac tcgttgacca attaccgccg cgccaattgc gccagcaaat tgcgcatcag
      481 gatgggtatt taccggcatt cgcaggtgag attccagcat ccgggcaaac gtctggcaat
      541 gactaacgcc accagtaaac agaatcggcg cttcacagga gagacgagca atgaaattgg
      601 cactccgccg cgccatcgcg ttaatcactc cggcgagaat cgcttctggc gcgacgcccg
      661 ctgagcgcag gctgatcact tctgattcag caaacactgt gcacatactc gtgatggcgt
      721 gcggcgtgac attttcggta atgcagtcga gttgctcgac gctggtgcca agcgtgcgcg
      781 agatcacctc caggaaacgc ccggtgcccg ccgcgcattt gtcattcatc aggaaatcgc
      841 acaggttacc gtcatcatca agctgaatca ctttgctgtc ctgaccaccg atgtcgatta
      901 ccgcgcgcgt tgctggcgca agaaaccgtg cgcccagccc gtgacaggag atttccgtca
      961 cttgtttatc ggcaaaatct accagttgcc gcccgtagcc ggtgagcgtc agaaacggcg
     1021 ttgtctctaa cccttcgcgc agagtttccc aggcttcagt aattgctgtt gccgggcgaa
     1081 agggggttgg aacgaggaaa cggcgcgtaa tcacgccgtc tgccagtaag atccctttgg
     1141 tggcggtaga accagaatca atgccaatcg aatatgtcac tgccactcct tacagcatct
     1201 caataaaggc cgcgacacgg gtactgagct gcccgacatc tgaggtggag tagtctgttt
     1261 caatagcgat ataaggaatg ttgtgctgct gacgcacatg acgtttaatt gccagcgatt
     1321 ccaccgcgta ggtatggcac gcctgcaaaa tcacatcaac tacgccatcg acctgatatt
     1381 cttccaccat ctggctgagc attttcaggc gctgatcgtt cggcgaaaca caggagcagc
     1441 caatcgccag atatttatcc gccagcgcgt cgtagacatc gcccgtttct gccacgcatt
     1501 gctcggtcgc tttcgccccg gtgcagtttt cataaccgac aacccagccg ccattctctt
     1561 caatcgcgcg caccactttc tctgctgcgc cgccaatcgg gcagccggtg attaaaatgc
     1621 gcggacgcgg gtccagtcgc tggccttctt cccgctgctg acgaatgcgg gcggtcatcg
     1681 cgtccagttc attgatcaac gcctctttat cgaaccggaa ggttgcgccg taaaccactt
     1741 tcagaatgtc gctgccgcta agcgccggag gatttaactg cccaagatga taaaaattgg
     1801 ccagtgcgcg acgttcgcgg tttttcagcg caatagcatc gcgcagagca tcttcgctaa
     1861 tctcgtgccc aaaacgttct tctaccgttt tttgcaagcg cagcatctcg gctttccaca
     1921 atgcacgcga ggcatcgtcc ttaacgctgt tcggcagctg catcacatga acaggcttaa
     1981 actccgccat gtattcatac attttctttt tgccgtcgca ggtggtttca ccgaccacca
     2041 gatccgaaaa gtagaagtag gggcatttat cggttttgcc gaagccgtag ctgcttttaa
     2101 tcagcgggca gaggttgcgc ggcagatctt tctccgcttc ttcaatggtt tcatcagagg
     2161 tggaacagag cgaaaccaca accgcaccgg ctgccatcgg gatctcttgc ggcataaagg
     2221 tgcagtaagt gccaaccagc ggaatgccgc gctccttgag atccatgacg gtgagaaagc
     2281 ctttctggcg agcttcagag aactgatcga aaatggcggg tagatcggtg acaagtgaca
     2341 tgattttcct tccccgtacc acgggagata atgaaaagag gccacattat taccattctt
     2401 ttagtatggt ttatttgatc tctctcgcgg ttagccactt agtttttcat ggatttctaa
     2461 aaattgccga gggggaacaa ggccgggagc tgcgacagca aatataaaat tagcccaata
     2521 caaccgccaa ccagcgtacc gttgacacga ataaactgca gatctttgcc gatatttaac
     2581 tctatttgcc gcgacatatc ccgggcatcc cagcttttta ccgtatcgct gatgtggcgc
     2641 gtcaggaatg cggaaaactc aggcgcgacg cggtgcgcgg cttgttccag atgaccattt
     2701 aacgacgccc gcaaggcatc atcggcaatt aacgtttcgc caaaccattg acccgcccgc
     2761 gcgatacgtt ctttcacacg agaatcttca ctgttgatat ccgctttcag ccattcccgt
     2821 aaatcccccc acaattcact gagatagcgg ttaaaagctt catcttcttt cagataactt
     2881 tttacggcat cggctcgcgc tgccatttcc ggatcgtttt tcagcttgtc gatcagggca
     2941 aaagtggcgc gatcaaacgc atggcggatc tgatgcgcac gatcgcggct gatatcatca
     3001 agcaaagaat tcaccgcgtc agaaaccaac tccgcgctat gttcgcccag ccattcggtg
     3061 gggagaattt tggctttcag tggatgctca ctctccagcc agcgaacaat ttgctgggcg
     3121 ataaacttgc gcgatttatc gcgctggaga agagcaatca actgtgcgat cagcgtatcc
     3181 agcagcacct gatgacgatc gtttttggtc atactctcca gcatcaacgc actggtgccg
     3241 gaaagatcga ccttatcaat cgcccgatgg accgcgcgct taagcaggcg ctgaatacgc
     3301 gcatcatcag ttaattcgag aaaaccactc atgatctgca acagatgctg accaacgcgg
     3361 cgggcgtttt ctggctggct aaaccagttg ccaatcaaca acgccggttc gtggcgtcga
     3421 atcaatgcca ccagggattg agtatcaaga aatttttcct gcacgaactg gccgagattt
     3481 tcgccaatcc ggtctttatt acgcgggata atcgccgtat gacgagaaat aatcggtatc
     3541 ggcacgcggc gaaacagcgc caccaccgca aaccagtccg ccagcgcgcc gaccatcgcc
     3601 gcttcagcaa tcgccttcac gccgctcacc caaaaattgg gcggcaaaaa cagcgtaacg
     3661 acaaaggtag cagcggcgat aagcagtaaa gagagcgcca acattttggc gcgtctgagt
     3721 tcaatgagtt tattcataac gttaagaata gagcctgacg gcaggatcgc gcaaaaatca
     3781 ctgctcctcc gccagctcgg cagcctgatg ttggcgcacg cgattgagca atcctgccag
     3841 ggtgaaaacg acgataacgc ccgccacgac ggctaacaga tgaaacggca agcgaccgcc
     3901 gttaaaccac agccagcggc cgatttcgac cgagacagac atcaccatca ggatcaccat
     3961 attcagcgat gcggagacgg tccctttcgg taagttattg gaaaacagcg taaagcggaa
     4021 taaggtcggg aaaatcaaac caatcccgaa agcatacaga ctggtgccca gcatcgacca
     4081 cagccagacg tgcggtgaca gcagattgcc gataatcaac agcgcgaggc caaccagttg
     4141 aatgggtacg gcacgccaga taaagcgcgg ttcggtcgga tctttaacga aacgcgccac
     4201 gatggcattc gcgacaatca ccgcgccgaa caccggaacc tgtgtccagg cgaactgcga
     4261 ggtcgttaag ccgcctgcgt caatcaggat caccggcgag acagccaccc aactcatcat
     4321 ggggatatag cttaaagaga tggttgctgc gccaaagagg aacagccgat tgcaaaagac
     4381 attacgaaaa tcgcgcaaga cgcttttggc gctaaatgga accgcgccgc gcttcaccgt
     4441 ctctggcatc gccagcagta agccaacaaa tgagataaaa cccataaccg caatgatggc
     4501 aaaaaggact ttccagtgca caaagtgcat cagagctgct ccggaaagcg ggccaataat
     4561 cggcgcaatc agtacgatgg aggtgataat cgccatcaac ttgatccctt ttgtctgtcc
     4621 gaacgcctcc tgcaccgtga cataaccaac ggtagcaata aaacagatac tggtgccctg
     4681 aattgcacgc gcgataagaa actgtgtcat agacgttgtg aacattgtcg cagcgcaggc
     4741 aagggtaaaa atgagcgccc cggtaatcag cactggcctg cggccaatcc tgtcggaaag
     4801 tggccccagc agccactgta acgccatacc gccagcgaga tagagactga cggcagcagg
     4861 ggccagactg acatcggcat taaaatcacg gaccacatta atgatccccg gctggatcag
     4921 atccgtcgaa agataggcgg caaagtcata caaaatcaac gccatcggga aaaacagcgt
     4981 agcggcatgg cgggcaaaaa aacgtggcat aacaaacaac tccttgtccg gagaacccca
     5041 atggggaaaa cgaaattcaa taccgtcggt aatgacgaaa ggcaggcgag aaggacaaat
     5101 gtactgaatt tcatgtaatc aatcacactg cctgtgaaca cacagacaga gaaatacctt
     5161 ctgacttcgg gtcagaatgt ataacgatta caaatagtca tcagcacatt acacctcttg
     5221 aattgatggg ttgcatagaa aatatacacc ttaagtgtaa ttaaaatttg cggttaatca
     5281 aaagagcgcg gtggaaaggg gaatatctgc cgggtacagg acatgtaaaa cgcgagcctg
     5341 tttccggaaa aatgtatcgt ttgcagacga acaaaaatag cctggaaagc acctcgggga
     5401 acgagaaatt gccgggtgag aatggttttg ttagtcgcta aagtcgggcc atcttatcta
     5461 caggtgacgg atcgccatga caaacttcac gaccagcacg ccacatgacg cattatttaa
     5521 gtcctttctc atgcaccctg acaccgcgcg ggattttatg gagatccact tacccaaaga
     5581 tttacgtgaa ctgtgcgatc tcgacagctt aaaactggaa tccgccagct ttgtcgatga
     5641 aaaattgcgg gcgctacact ccgatattct gtggtcggta aagacccgtg aaggtgatgg
     5701 ttatatttac gtagtgattg aacatcagag ccgcgaggat atccatatgg cctttcgcct
     5761 gatgcgatat tccatggcgg tgatgcagcg ccatatcgag catgataaac gccggcagct
     5821 accgctggtc atcccgatgc tgttttatca cggtagccgt agtccttacc cctggtccct
     5881 gtgctggctg gacgaatttg ccgacccgac tactgcacgg aaactttata ctgcagcgtt
     5941 cccgctggtg gatgtcactg tcgtgccaga cgacgagatt gtgcagcacc gcagagtcgc
     6001 cctgttggag ttgatccaaa agcatattcg ccagcgcgat ctgatggggc ttatcgatca
     6061 actggtaata ttactggtta cagagtgtgc taatgacagc cagataactg cgctgttaaa
     6121 ttacatttta ctgactggcg atgaagcgcg ttttaatgag tttatcagtg aacttacccg
     6181 tcgaatgcca caacaaaggg agcgaataat gacgattgca gagcgaattc ataatgatgg
     6241 atggctgtta gggatggaaa aggggaaaga agaaggggaa caacgcctcc ttagattgct
     6301 tttgcagaac ggtgcggatc ctgaatggat acaacgatat actggacttt cggcagagca
     6361 aatgcaggca ttagagcaac ccttgcctga aagcaagcgc gatccatgga tcgagtacta
     6421 atcagagact gatgacaaac gcaaagctgc ctgatgtgct acgcttatca gacctacatt
     6481 acctctgcaa tctattgaat ttgcgcgatt tgtaagccgg ataaggcgtt cacgccgcat
     6541 ccggtatgaa caaagcacac tttgcctaag ccatgctgga acgtggcgat gcccctgcaa
     6601 agataaaacg agtcacaggc gattcgtgag aggaaatcag gggcctcgcc actaattcga
     6661 attcattcca ttgcctgata cacggcctcg ccaatttgct tcagtgcttc gcgataggtt
     6721 tcgctgagcg gcaaagcgca gttgatacgt agacaattac ggtatttgcc ggaagccgag
     6781 aaaatcgagc ctgccgccac ctggattttc atgcggtata gctgccgcgc gacgcagacc
     6841 atatcgacct gttcaggcaa ttcgatccac agtaaaaatc cgcctttcgg gcgcgtaata
     6901 cagatttcgc agggaaaata ttcccgtatc cagcaggtat aaagcgccaa attgcgctga
     6961 tagatctgcc gcatccgccg gatatggcga tgatagtgac cttccagcac aaacgtcgcc
     7021 gccgccattt gcgtagacgg tacattaaag ctgctgatgg cgtatttcat atgcagcagt
     7081 ttatcgtgat aacgccccgg tgcgacccag cccacgcgca ggccaggtgc aatactttta
     7141 ctgaacgagc tgcacaacag cactcgcccg tcgatatccc atgaatgaat ggtccgcggg
     7201 cgcggatact ccgttgccag ttcgccgtag acatcatctt caaaaatcac aatatcatga
     7261 cgctgagcga gagagagaac ggcccgtttg cgcgcgtccg gcataataaa tcccagcgga
     7321 ttattacagt ttggcaccag aatgatgcct ttaatcggcc actgttccag cgccagttcc
     7381 agcgcttcaa cgctgatgcc agtttctgga tcggttggga tttcaatcac tttcacgccc
     7441 atgccgcgca gcatctgcat cgaaccgtaa taacagggag attcgaccgc gacaatatcg
     7501 cccggtttac acaccgccat taacgccagc gacatcgagt tatggcagcc gctggtgatg
     7561 atgatgtcat cggcggtgac caccgagccg ctgtcgagca tcaggcgggc aatctgctct
     7621 cgcaataccc gctgaccggc taacaagtca taaccgagaa cggtttgcag attatgctgc
     7681 accacccggc ttagctcacg ccacaacggt ttcaggctgg gcgtttcgac atccggcgtg
     7741 cttttgctta acggaacaat ggaactgtcg ctatgcgcct ccagcatatc cagcacctga
     7801 tcccactggg taatttccac cgggcgctgc accggacgcg tcatcggcgg cactggcggc
     7861 tgggctttac gttgtgcgac aaaatacccc gaacgcggct gcggagtgat gagcttcatc
     7921 gtctccagcg tctgatacgc ctgctgcacg gtactgatgc tgacgccgtg ctcctgactt
     7981 aagctgcgca ccgacggcaa tttctccccg tgacgataca gcccctgctc aatccgctcg
     8041 gcaagtagag tcgccagatg ttgataacgc gtcatgctgt attccttatt tagaccatac
     8101 agagagaaaa accggtacag atgaggctaa aaaaccggat gcagatgctg ttttaacctg
     8161 tctgtatgtt aaataaaact aatatttgca tctgtattgt gaactgccag aaggcggatg
     8221 atgaagcctc tgaaagatga ggaggcagca gatggaattt cacaaaaaca aagccaaaac
     8281 gccgtttatc agacttgtgc tactctggca tgcgatgagg cgttggcggc ggcaaatgca
     8341 gacccgacga gtgttacagc agatgagtga tgagcggttg agggatattg ggttacgcag
     8401 ggaggatg
//
LOCUS       DAZKFU020000202         8392 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZKFU020000202 DAZKFU020000000
VERSION     DAZKFU020000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.204"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1653
                     /gene="espL4"
                     /locus_tag="ACHOQQ_RS18655"
                     /old_locus_tag="pgaptmp_003731"
     CDS             <1..1653
                     /gene="espL4"
                     /locus_tag="ACHOQQ_RS18655"
                     /old_locus_tag="pgaptmp_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HGU4471936.1"
                     /translation="YSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKA
                     YFDKMESNGISVMAAILLVDNHALTARLRIKNTTEGYTHYVVSVYDPNVTNDKIRIMS
                     ESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDEITPP
                     LSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSINLPGLYLAINYGNAD
                     IVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSILNALP
                     KLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADMLKIVLDALPLLIRTCHLTK
                     EQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISASDIVDLLTAK
                     SLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYPGLFS
                     AIQHKQQNVVETVYLALSDHARLFGFTAEDIMGFWQHKAPQKYSAFELACELGHRVIA
                     ELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1622..3358)
                     /gene="aceK"
                     /locus_tag="ACHOQQ_RS18660"
                     /old_locus_tag="pgaptmp_003732"
     CDS             complement(1622..3358)
                     /gene="aceK"
                     /locus_tag="ACHOQQ_RS18660"
                     /old_locus_tag="pgaptmp_003732"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGU4471937.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFYPEHGWEALLMRVLSDL
                     PLRLPWQNKSRDINYIIRHLTETLGPENFSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDYGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRTLQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3542..4846)
                     /gene="aceA"
                     /locus_tag="ACHOQQ_RS18665"
                     /old_locus_tag="pgaptmp_003733"
     CDS             complement(3542..4846)
                     /gene="aceA"
                     /locus_tag="ACHOQQ_RS18665"
                     /old_locus_tag="pgaptmp_003733"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGU4471938.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4876..6477)
                     /gene="aceB"
                     /locus_tag="ACHOQQ_RS18670"
                     /old_locus_tag="pgaptmp_003734"
     CDS             complement(4876..6477)
                     /gene="aceB"
                     /locus_tag="ACHOQQ_RS18670"
                     /old_locus_tag="pgaptmp_003734"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGU4471939.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6746..7675)
                     /gene="metA"
                     /locus_tag="ACHOQQ_RS18675"
                     /old_locus_tag="pgaptmp_003735"
     CDS             complement(6746..7675)
                     /gene="metA"
                     /locus_tag="ACHOQQ_RS18675"
                     /old_locus_tag="pgaptmp_003735"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGU4471940.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7832..8275
                     /locus_tag="ACHOQQ_RS18680"
                     /old_locus_tag="pgaptmp_003736"
     CDS             7832..8275
                     /locus_tag="ACHOQQ_RS18680"
                     /old_locus_tag="pgaptmp_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4471941.1"
                     /translation="MVISIRRSQHEESEKLIAIWCRSVDATHDFLSAEYRTELEDLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TSNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2118 a         2056 c         2085 g         2133 t
ORIGIN      
        1 tactcttcag ctgaaaaatt tctcacgcac ttcaacaaaa aatgcaaaaa ccagactttg
       61 gcgttgattt ccagccgtcc tgaggggcgt tgtgttgctg cctgcggtga tttcgggcta
      121 gttatgaagg catattttga caagatggaa tcaaatggca tcagtgttat ggctgccata
      181 ttattggtag ataaccatgc tttgacggcc cgactaagaa taaagaacac aactgaagga
      241 tatacccatt acgtggtttc ggtttatgat ccaaatgtaa ctaacgataa aataagaatc
      301 atgagcgaaa gtaaagagga tattaaacac tattctctga tggattttat gaatgtagat
      361 tatagcctcc tgaaatggtc aaatgatcat gttattaacc aatctgttgc aataattcca
      421 gcacttccga aagaacagct attgatgtta aaaggatctg tggatgaaat aacccctcca
      481 ttatcacctg caacgatgaa tttgctaatg gcaattggtc agaatcacca acttacgcaa
      541 ctgatgattc aactccagaa aatgccagaa ctacatagaa cagaaatgtt gactgcctat
      601 aatagtatta acctccccgg tttatatttg gctataaatt atggtaatgc ggatatcgtt
      661 gagactattt ttaattcatt gtcagagcca ggatatgaag gattactctc gaaaaaaaat
      721 ctcatgcata ttctggaggc aaaagataaa aatggttttt ctggattatt tttagcgata
      781 tcacgtaagg ataaaaatgt tgtaacctcg attctgaacg ccttacctaa actggccgca
      841 acacatcatt tagataacga acaagtgtat aaattcctga gtgccaaaaa tagtacgtcc
      901 agccatgttt tataccatgt tatggcgaat ggtgatgccg acatgctgaa aattgttttg
      961 gacgcgttac ctttgttaat tcgcacatgt catttgacta aagaacaggt actcgatctc
     1021 ctgaaggcaa aggattttta tggttgccca ggactatacc tggcgatgca aaatggacat
     1081 agcgatatcg tgaaagttat tcttgaagca ttgcccagcc tggcccagga aattaacatt
     1141 tcagcttccg atattgtaga tcttctgacc gctaaaagtc ttgcgcgcga cacgggtttg
     1201 tttatggcca tgcagcgcgg acatatgaac gttattaata ctatttttaa cgcattaccc
     1261 actctgttta acacgtttaa attcgataaa aaaaatatga agcccctcct cctggcaaat
     1321 aattctaatg aatatcccgg tttgttttca gcgatacagc ataaacaaca aaacgttgta
     1381 gagacggttt atcttgcttt atctgaccat gcacgcctgt ttggatttac cgctgaagat
     1441 attatgggtt tttggcaaca caaagcgcca caaaaatatt ctgcctttga gttggcttgt
     1501 gaattgggtc accgggttat tgctgaatta atctttaata cattaaataa gatggctgaa
     1561 agctttggct ttacggataa ccctcgatac attgcggaga aaaattatat ggaagcttta
     1621 ctcaaaaaag catctcccca taccgtacgc taaatctttg cctgcgccga tacgcataaa
     1681 catcttccac atgtccttca cgaatgcggt tttgtagtgt gcgccagtaa tcagcgcgga
     1741 acaggtcggc gtgcatctct tcaaagagtg gcccaatgcg ggggtcggcg catagccagt
     1801 ggcggaactc ttccgggaaa acatcgcccg gcgagacgct gtaccacggt tcgctggcaa
     1861 gttcgtcttc cggatagcgc ggcggcggga tgtcgcggaa gttcacttcc gtcatgtagc
     1921 aaatttcatc gtaatcataa aaaaccacac gcccgtgacg ggtgacaccg aagtttttaa
     1981 acagcatgtc gccagggaaa atgttagcgg cggcaagctg gcgaatagcg ttaccgtatt
     2041 cttcaatggc gtcacgcaac tgctgacctt ccacttgttc cagccagata ttgagcggca
     2101 ccatccgccg ctcaatataa agatggcgaa tcacaatttg ttcgccgaga tcggtgattt
     2161 tttccgctgc ttcctggagc agtaattcca ttaatgccgg ggaaatatgc cgcttctcca
     2221 gcacaaagtt ttcaaactcc tgggtgtccg ccattcggcc cacgcgatca tgctctttca
     2281 ccagttgata gcaggcacga acgtgagcgg cagacatctc tttctgcggc gcgaacctgt
     2341 ctttgatgac tttgaatacc cgatcaaagc ccggtagcgt aaacaccaac atcaccatgc
     2401 cacgaatacc cggcgcttca atgaactgct cattacagcc ctgtagataa acgagatatt
     2461 cgcggtagct ctcggttttg gcatgcttct ggcagccgat agccatatac aattcagcgg
     2521 tggttttacc tggcagaatt tcccgtaacc actcgaccag cgctgcgggc agcggcgcat
     2581 aaaccataaa ataagaacgc gcaaagccaa aaacaatgct cgcttcggcg gtcgtcgtca
     2641 ggcaggtatc aataaataac tcgccgtagt ccgtctggtg gatcggcagc aaaaatggca
     2701 atgtgccgga aggcgtgatc agtttgccta ccagccaggc ggctttattg cggtaaaaca
     2761 gttcgttcgc cacctgtaaa tgacttttgg agaagttttc tggccccaac gtttccgtca
     2821 gatggcgaat aatataattg atatcacggc ttttattctg ccagggcagg cgcagcggca
     2881 ggtcactgag gactcgcatc agcagcgctt cccagccatg ctcggggtag aagtctttcg
     2941 ccagtgggcg cggaatggta cgaaagcggc gctctggctg agagctaaag ataaaaagcc
     3001 gctcgggagt aagcgagcgg tggtcaaata accgacagta cacggagtta aaaaagctct
     3061 ccgcaatctc gaagcgcggg taatccggca acagccgggt gtaatgctct ttgacgcgca
     3121 gtaaaaattc cgcgtccgtg ctttggccgt tggtaatgca gcgcagttgc tccacgacca
     3181 gaccaacgtg atgatcgtaa agatggatac ggtttttcat cgcctgctgg acagcatgcc
     3241 agtcggcctg ttcgaaacgc tgctgcgccc cggaagtcac ttcgaggaat cgaccatact
     3301 gagcgtcgaa gccttgcaaa atggtttgag caatcagtaa ttccaggcca cgcggcattt
     3361 agcgccctca tcaggagcag agaattgccg gatgcggcgt aaacgcctta tccagcctac
     3421 gttcggcaac ggttgtaggc atgataagac gcgcaagcgt cgcatcaggc atcgtgcact
     3481 gattgccgga tgcggcgtaa atgccttatc cggcctacag tcagcaacgt ttgtaggttg
     3541 cttagaactg cgattcttca gtggagccgg tcagcgcggt gactgaagac gtgccgccct
     3601 gaataatggt cgtcacttta tcgaagtaac ctgtacccac ttcctgctgg tgagatacga
     3661 aggtgtagcc atctttcgcg gcggcgaatt ccggttgctg cactttctca acgtagtgct
     3721 tcataccctc gccctgggca taggcgtttg ccaggtcgaa catgttgaac cacatgctgt
     3781 ggatccccgc cagggtgatg aactggaact tgtagcccat atctgacagc tgctgctgga
     3841 agctggcaat agttttgtcg tcgaggtttt tctgccagtt gaacgacggc gagcagttat
     3901 aagccagcag tttgcccgga tatttcgcgt ggatggcttg tgcaaagcga cgcgccagtt
     3961 ccagatccgg cgtggaggtt tcacaccata ccaggtcagc atatggtgca tacgccaggc
     4021 cacggctgat cgcttgctca atgcccgcat gagtacggaa gaagccttca ctggtacgct
     4081 cgccggtaat aaattcgctg tcatacgggt cgcaatctga ggtgatcaga tccgccgcat
     4141 cagcatcggt acgtgcaacc agcagggttg gaacgcccgt cacgtcagct gccagacgcg
     4201 ccgcgaccag tttctgaata gcttcctgag ttggcaccaa aactttgccg cccatgtgac
     4261 cgcatttctt cactgacgcc agctgatctt cgaagtgaac tgccgctgca ccggcttcaa
     4321 tcatcgcttt cataagttca aaggcattca ggacaccgcc aaaaccggct tccgcgtcgg
     4381 caacgatcgg caggaagtaa tcgacatagc gcggatcgcc cggctcaatg cccgcggacc
     4441 attggatctg atccgcacgg cggaaagtgt tgttgatccg ctccaccaca gctggcaccg
     4501 agtttgccgg atagagcgac tgatccggat acatgctggc cgccaggtta gcgtccgccg
     4561 ctacctgcca tcccgacaga tagactgctt caatacccgc tttcgcctgt tgcagcgcct
     4621 gaccgccagt cagtgcaccg aggctgttga tgtagccttt tttcgactcg ccatgcagca
     4681 gacgccacat tttcgccgcg cccagttgcg ccagcgtgca ttcaggattg actgaaccgc
     4741 gtaatttcac cacatcttcc gcgctgtatg ggcgagtaat gccttcccaa cgcggttgag
     4801 tccactcttt ctgtaattct tcaatttgtt gtgtacgggt tttcatgtgc agatgctcca
     4861 tagttatgtg gtggtttacg ctaacaggcg gtagcctggc agggtcagga aatcaattaa
     4921 ctcatcggaa gtggtgatct gttccatcaa gcgtgcggca tcgtcaaaac gcccctggga
     4981 gaaacgttct tcgcccagtt cgctggcaat gactttcatc tcttcgccca gcatctggcg
     5041 gaacaaggct ttggtcaccg gtttgccatt gctcaacgtt ttttgatgat ggatccactg
     5101 ccagatcgag gtacgggaaa tttcagccgt cgccgcatct tccatcaggc cataaatcgg
     5161 cacgcagccg ttgccggaga tccatgcttc gatgtactgc acagccacgc gaatgttggc
     5221 gcgcatacct tcttcggtgc gttcaccatc acaaggtgcc agcagctgat cggcagtaat
     5281 cggcgcgtct tgttcgcgca tcacttcaag ctgattttta cgggagccga gaatgtcgtt
     5341 aaataccgcc attgccgtat ccgcaaggcc tgggtgagcg atccatgtgc catcgtgacc
     5401 gttattggct tccagcgatt tatccgcttt tactttgttg agcacttggt tattgcgctc
     5461 ttcatctttg ctcggaataa acgccgccat gccgcccatc gcaaaagcac cgcgtttatg
     5521 gcaggtttta atcaacaggc gtgagtaagc attcaggaat ggtttatcca tcgtcactgc
     5581 ctgtctgtct ggcaggacgc gatcgggata gtttttcaac gttttgatat aactgaagat
     5641 gtaatcccaa cgaccgcagt tcagaccaac aatatggtca cgcagtgcgt gaaggatttc
     5701 gtccatctgg aacacggcgg gcagcgtttc aatcagcaac gtcgccttga tggtgccgcg
     5761 cggcagatta aagcgatctt ctgcatagct gaagacttcg ctccaccagg ccgcttcctg
     5821 ccaggactgg gttttcggca gatagaaata gggaccgctg ccctttgcca acagtgcctg
     5881 atagttgtgg aagaaataga gcgcaaaatc aaacaggcta ccggggattg cctcaccacg
     5941 ccaggtgaca tgtttttccg gcaagtgcag accgcgtacc cgacaaatca aaaccgctgg
     6001 attgggcttg agctggtaaa ttttgcctgc ttcattcgtg taactgatgg tgccgttaac
     6061 cgcatcgcgc aggttaattt gcccgtcgat cactttgttc cagtctggtg ccagtgaatc
     6121 ttcgaaatcg gccataaaga ctttcacatt ggcgttcagt gcgttgatca ccatcttgcg
     6181 ctctaccggg ccagttatct ctacgcggcg gtcttctaag tccgcaggaa tcccgcgaat
     6241 tttccaatca gcatcgcgaa tggaagctgt ttccgaaata aaatcaggca acgttccgtt
     6301 atcaatatct tgctgctgct gaatgcgcgc tgccagaagt ttattgcgtt gtggcgtaaa
     6361 atgcgtcacc agctcagtca gaaattctac cgcttcggca gtaagaattt gcttctcctg
     6421 ctcgccatac ggccttgtga aagccagttc atcggttgtt gttgcctgtt cagtcatcgt
     6481 gcagctcctc gtcatggatc cgaaaacttc cccactgtga acgaaggatc gttgtgcgct
     6541 tttcgttcag cacaactaag actactcatt taaaatgcaa aatcaaaaac aatttccatt
     6601 ttaattaaaa atacttgata acttattgat aacaatatga ataaaattta ttcacctact
     6661 gaggtgtgtt tcggaaataa aaaaggcacc cgaaggtgcc tgaggtaagg tgctgaatcg
     6721 cttaacgatc gactatcaca gaagattaat ccagcgttgg attcatgtgc cgtagatcgt
     6781 atggcgtgat ctggtagacg taatagttga gccagttggt aaacagcaaa ttaccgtgac
     6841 tacgccagct cgctcgcggt gtattttgcg gatcattgtg cgggaaatag ttatacggta
     6901 catccgggtc tagtccggct tccacatcgc ggaaaaattc ctgcgccagc gtttgcgcat
     6961 catattcggg atggcccgtc acaaaggcaa tgcgcttatc tttgctggca aacagatatg
     7021 catccccttc ttccgtctct gccagaattt ccagatcggt ataatcacga atcaacgctg
     7081 ccggaaagtc agcatagcgc gaatgtggtg ccaggaatga atcatcaaag ccacgcgtca
     7141 gaagcgcatg aggatggaga atatgatgct cgtaaacgcc agagagtttg tcggtgcgag
     7201 tttgcttagg tatgccgtag aggatattga gcgcggcctg taccgcccag cagacaaaca
     7261 gcgtcgaggt gacgtgatct ttcgaccact ccagcacctg tttgatctgc ggccagtaag
     7321 cgacatcatt aaactccacc aggcccagcg gcgcaccggt tacaatcaga ccgtcaaagt
     7381 tctgttcctg aatatcttca aagttacagt agaagttgtt cagatgctct gcgggcgtgt
     7441 tgcgcgattc acgggaatcg atgcgcaaca gctgaatatc gacctgcaaa ggtgagtttg
     7501 aaagcaggcg cagaaactga ttttcagttt caatcttctt cggcatcagg ttaaggatca
     7561 gaaccttaag tggacgaatt tcctgaccag acgcacgaga agttgtcatc acaaagacgt
     7621 tttcttcacg caagaaattg acggcgggta gctcgtccgg cacacgaatc ggcataacct
     7681 gattacctca ctacatacgc ttatacgttt agacatccag atagctgaag ataaccagaa
     7741 aatttgccaa tgtcgagcct gcatgttgaa ggtgagaaag tttcaaggaa aagactgcgt
     7801 tagaatataa gaatgactaa aaggagagaa aatggttatt agtattcgcc gctcgcagca
     7861 tgaagaaagt gaaaaactga ttgcgatttg gtgtcgttct gtcgatgcca ctcacgattt
     7921 tttatcagca gagtatcgga ccgagctgga ggacctagtt cgttcctttc tgccggaagc
     7981 accgttgtgg gtcgcggtta atgaacggga tcagccggtt gggtttatgt tgttaagtgg
     8041 gcagcatatg gatgcgctgt ttatcgatcc tgatgtgcgc ggctgcggcg tgggccggat
     8101 gctggtgaag catgcgctct caatggcccc ggaactgaca agcaacgtta atgagcaaaa
     8161 tgagcaggcg gttgggttct ataagaaggt gggttttaag gttacgggac gctctgaggt
     8221 ggacgatttg gggaaaccgt atccgttgct gaatctggcg tatgtggggg aataagctgc
     8281 cccctatcgt gctatcgtgc ctgcgtaagc tggataagac gcgccagcgt cgcatccggc
     8341 atttttttgc accaacggca aaaaggccat ccgtcaggat ggcctttcgc tt
//
LOCUS       DAZKFU020000203         8390 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZKFU020000203 DAZKFU020000000
VERSION     DAZKFU020000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.205"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(86..2956)
                     /locus_tag="ACHOQQ_RS18685"
                     /old_locus_tag="pgaptmp_003737"
     CDS             complement(86..2956)
                     /locus_tag="ACHOQQ_RS18685"
                     /old_locus_tag="pgaptmp_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGU4471942.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(2953..3732)
                     /gene="ygfM"
                     /locus_tag="ACHOQQ_RS18690"
                     /old_locus_tag="pgaptmp_003738"
     CDS             complement(2953..3732)
                     /gene="ygfM"
                     /locus_tag="ACHOQQ_RS18690"
                     /old_locus_tag="pgaptmp_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGU4471943.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3783..5111)
                     /gene="ssnA"
                     /locus_tag="ACHOQQ_RS18695"
                     /old_locus_tag="pgaptmp_003739"
     CDS             complement(3783..5111)
                     /gene="ssnA"
                     /locus_tag="ACHOQQ_RS18695"
                     /old_locus_tag="pgaptmp_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGU4471944.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLKAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(5114..8212)
                     /gene="ygfK"
                     /locus_tag="ACHOQQ_RS18700"
                     /old_locus_tag="pgaptmp_003740"
     CDS             complement(5114..8212)
                     /gene="ygfK"
                     /locus_tag="ACHOQQ_RS18700"
                     /old_locus_tag="pgaptmp_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGU4471945.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         1927 a         2193 c         2214 g         2056 t
ORIGIN      
        1 tgagaacggc ttatctcatt ttcgcagtca ctatttagtc atcgttcaaa cagagaaaca
       61 ggccgccgaa gcggccattt attacttata ttttttccag cgcggtgagt attttctccg
      121 gtgtgaaatg ccattcgcgt aaccagatgc cgcaggcatc gtgaattgcg gtagcaatcg
      181 ccggagctgc gccatttaca ccaatttccg agatcgattt cgccccgaac gggccgactt
      241 tatcgtcgct cggcaccaac acagcgcgga aatcacgcgg aatgtcacca attttcggtg
      301 cgccataact gcgtaaatca cgcgttaacg ggtgaccttc ggcgtcataa atgatctctt
      361 cgctcatact gtggccgata gcacgcaggg tggcaccgta gatttgtccc aacgctaact
      421 ctggattgac cggtgtaccg cagtccagca aggcgtagaa tttatccagg cggatttcac
      481 ccgtacgcgt gttgacggca acttcagcga agtttgcgcc atacgggaaa gcgaaatcag
      541 gcgtgatata actgccagtt cccaccagtg aaccaaagcc ggtgccggtt tcgcctttat
      601 gggcaatatc cccgaaacta acttcgcctt tcttgccgcg cacgacgccc ggcgttgcta
      661 gttgaacatc tgccactggc tcacccaaca tttgcgcgcc gtggaacaga attttctccc
      721 gcagattttc cgctgccaaa cgcgccgcgt tacccgagaa gcaagtaccg gacgaggcat
      781 atgcgccttt atcaaacaac gcatgatcgg tatcaccgga gataacatgc acgtcctgcg
      841 gtgggcagtg cagcacttct gctgccagtt tcgtcactac agtatccaga ccagtaccaa
      901 tatccgcacc gccagaatga acgataaagg tgccgtccga ttccagtttg atcatgcagt
      961 tagcctgatc gatatccggg atacccgatt tctgcatgat aatggcgacg ccgcgaccga
     1021 tgtgccagtc accattttgc ggtttcggtg aagaccattg gatcatctcg cgaccctgac
     1081 gcaggatctc ttccagtgcg cagctggcgg cggaaggaac ggaggttggc gctttacctt
     1141 caccaattgc accgagaatt ttcagctctt gcccttcgtg tacccggtta cgttcgataa
     1201 tttccagttg gtcgatctgt aactgttcag caagttccgc taatgccatg gtgatagcga
     1261 agttaccttt cggtgcgcca taaccctgat aagcaccatt tgggcaaatg ttgctgtagt
     1321 aggtggtgac ctggaaatcg acgttatcgc acggatataa gggcagcgac agcgccggtc
     1381 cgttacacgg tacggtgagt gagtggttgc cgtaagggcc agtgttggcg cggaaatcca
     1441 tcttcactgc cgtcaggcga ccatcttttt tcgctcccag tttgacggtg actttcgcga
     1501 cgtgacgaga ggtgttagca ataaactctt cttcacgggt gtagcggaac agtaccggac
     1561 gcccggtcac gcaggttgcc catgcgcaca cttcttccag caggatgtcc tgtttggaac
     1621 caaaaccgcc gccaactcgc tctttaatga catgaacttt atgctgtttc atgcccacga
     1681 ggcgcgcgac ctggcggcgt aagtgccatg gtacctgggt ggaggcgtgg ataaccagac
     1741 gatcgccatc catgcgggta aagcagatat gtgtttcagt cgggcactgc tgcgcctgcg
     1801 ttgagttata ggttcgctca atgatcacat cggcatcggc aaagcctttg tccatatcgc
     1861 caatatgacc atgaatactg gcggcgatat ttttgcgggg gcgagaaccg atcgggaagt
     1921 taatgatcat atgctcgccg cgctgggctg catggctgtt atcgtcttcc agagtatctg
     1981 gcgcaccagc aacatacacc accggttcat cgtgcacgac aggcgcatct tccgccattg
     2041 cttcgtcgat cgacattacc ggcttaagca cttcatattc aacgtcgata agcttcagtg
     2101 cttcgagcgc aatttcttca ctttctgcga ccaccgcagc aacacgatcg ccgacgtgac
     2161 gcattttctt gccgaacata cggcggtcaa gcggtgacgg ttccggtgcg ctctgccccc
     2221 ccggggtata gtagatatcc gggcaattca gatgagtaat aacgtgaacg acgcccggta
     2281 aggcttcagc tttgctgaca tccagatgag taatcagtgc gtgagcgtgt gggctacgta
     2341 acattttaat gacgcaggcg tcagccgtta cgcggtcttc aacatagcag ggcttcgcct
     2401 gcaccatttt tgcggcatca gttttaggat aatgcttacc aatgacttca aggtcgtcac
     2461 ggaaagtcgg agcgatatca atggtggcct gcggattatt tttacgtgca accgccagtt
     2521 caatgacctg atagtattgc tgccagccag catcgcggct aaacagacct gagagcgcgt
     2581 cgtcgatctc ttcgcgggta ggtgcggcga tgcgatcgag aagatcggtg ataatcagag
     2641 ctgcagctgg atcgttataa ccagactgca ccacgccaac atcaaccatt gcctgttgaa
     2701 ccagacttaa ctcgttccat ttgcccagag attctgcggt acgaatatct gccttctcta
     2761 actgtgcggc aataagcaag gacgcgttaa cgatattacc gttaaagata attgcgtcag
     2821 aaccggcaaa cccgaagcca tcatcactgt tgcgtacaga gtgcattccc atgttaaaca
     2881 acagcttttg cacgttttcg cctggattaa cggttagctc ctgaggcgcg ccatttaaag
     2941 taaagtggat gatcatacgg cttcctcccc agcctgttgg cagtcggcat acaggtcggc
     3001 taccagaact cccgtgatat agcgtttata ggccacgctg ccccgcaaat cttcctgcgg
     3061 gaaaatggcg ttggcgacag cctgttcaag tgcattgcct tccagatttt gtttttcgac
     3121 atcgtgcaga cgcagtgctt tactggcgac gccatccagc gcaattcgca taccgtcgtg
     3181 gtctgtcatt gcaacggctg ccgtcacgac ggttaaacct gcctgagaac ggctaatttt
     3241 ccgggtcgca caggtgcgat acggatcttt aatgataatt tcggttagca ggcgatcgca
     3301 tgggcaggcc aggtagtcct cgattgacag cgtttcgccg ttgccaaaaa ccagttcagc
     3361 atccagtgcc agcaggacgg gaagcagcac cgactcttcc tggcgggcgg cgatttcacc
     3421 accaatggtc gactgattac gaacatggcg tgagtaaaca aaaccgaggg cttcacgcag
     3481 cgctgcagga ataaatcgcg catcacgcag tggctgcaag cgagacattg cgccaatccg
     3541 cagtgcaccg ttatcccagt caacccagtc cagttccaga tcctgtaagg agatggcaat
     3601 ctttttatcg gtacgggttg gtgtagcatt gagtttgctg cccccggcga accagacggc
     3661 ttcatcctgg tagcggcgct tcagttccag cgcctgttcg acagagtcgg gcctgaaaaa
     3721 ttgttcaatc atcttctttc cttatttaac cctcttccct gcgggaagag ggcatctgtc
     3781 atttatgcca gcgcatccat ccgacgccac atactggcag cggcttttct ggcttgtgca
     3841 taaatggaat cgcaatcgaa gttaaactga cggtcttcat agaccatcac accattgacc
     3901 atcacgctgt gaacgctgcc tgagcccata ccgaaagcga tatgcccggc gatattgtct
     3961 gccagcagcg gcgtcggcga gttgtaatcg caaatggtta aatcagcttt gtaaccggct
     4021 ttcagaagcc caaatttcgc gccaaagttg cggctcatca gttcgttacc gttagtcagg
     4081 gctttggcaa aactgtcagg ccacagcgga ccacccgcat cgcgatgttt aaagaaggca
     4141 aatttcatct cttcaaacat gtccgaacca atgccgtccg ttcccaacgc cagattgcgg
     4201 atgtcgctaa ggtgatggtt gtagccgaca tggttgttca tgtttgaacg ggcgttatgc
     4261 accaggaacg catcgcgctg attgagtagg gtgatgtcat ctttcgacaa gtacagccca
     4321 tgagcgacca gtgttttgct gtcgatgaga tcgaattgcg ccagtcgtgc cagcaggtct
     4381 ttgccgtacc agtggtgact gtaggaaacg tcgtaaaggt cttccgcagc gtgaatatgc
     4441 aaaccacggc ctgtggcttt cacggcttca cgcagcatct ccagaccggc atctggcacg
     4501 gtaaacggcg cgtgagcacc gatatgtgct tccaccagat acggctcggt tgctgctttc
     4561 ttcgcctcat caatctgacg ggcaaagcgg atgttttctt ctacaccttc ctgcaactct
     4621 ttgatgccgt tgttacggtc agtagtttca aaacaggtca tcgcgcgcag gccaactttt
     4681 aaaaatgcgt cgcgcaatgt ggagagcgac ccgccgatat acgccggaga ggcatggtga
     4741 tcgataaccg atgtacatcc gctcttaatc gcttccaggg aacaaatcag tccgctgtaa
     4801 tagagcgact cttcatcaag ggcgcgatcg agccgccacc agagattttt cagcgttgag
     4861 atgaaatccg ggcaaggggc gatgtttgcc ataattccgc gggaaagccc cgagtaaaaa
     4921 tggttgtgcg agcagacaat tcccggcatc acaatccgac catgcatctc tttgaagctg
     4981 gcgtcggggt agcgttgcgt cagggcatcg ccgatagcga caatcacatc gttttcgatg
     5041 gcgatatcaa cgccttcctg cacttttgcc gggtgtagct gtaccgcagt gacattcttc
     5101 agaatcaaca tgattacacc tccacgcggc ccagcagata atgatgatgc tggtggacat
     5161 ggctgatgat gcggcacata tcgttcagct ccggtggtac gttgttaaac tgacctttgc
     5221 tgtcgatgtt taacacccag ctttggttat tcagacgtac tcgtacccgg caatcttcca
     5281 caaggaagcc tgggttgctg ctgttatcaa agtcttgcgc caggctgaag acggtgattt
     5341 tgtctttgta cggtttaccg ttccacggac agaactgagc gcagttgccg cattcgttac
     5401 agtaagcgtc gaggtgcagc gtctggaaac ggttctggaa gcctgggacc gcaatggaga
     5461 cgttggcgcg gttcgggcag acatccacac acttgctgca aacgtagtta cattcgaggc
     5521 agcgagcggc ttcctgggcg acaaacgcgt cacgatcgtc actgttcacc agcgtgatag
     5581 agatatcgcc tttacgttga tagatttccg ccggattgac gttgttccag tatttatcgt
     5641 tctggtggga acggatattt tcccgactaa ggatggcatc ggtcgcccga cgcgcggttc
     5701 cgacagcagc gacaatggag gatggtccgc gctgtacgtc gccgatcata aagacgtcag
     5761 tcagacgagt ttcgccatta tggtcgacgt ctggccagcc gtttttgtcc agcggcacgc
     5821 ccatcgcatt cagggcttca gtatcctgct gttcaccaat ggcggtgatc aggctgtcta
     5881 catgcagtgt tactgtttcg ttggtttcaa ccggacgacg acgacctttc tcatccggtt
     5941 cgccaagcga cataacgcgc aaggttaagg tgccatcagc atcgaaacgt tccggattgt
     6001 tcaggaaacg gaactccacg ccgtcgtgca acgcttcttc atactcttcg cgccatgcgg
     6061 gcatctcttg cagtgaacga cggtaaacga tcgttgcttt ttctacgcct ggaacgcgta
     6121 acgccgcacg agcgcagtcc attgcggtgt tacccgcccc gacaacgacc acatgtttgc
     6181 ccagcttgag cgctgtaccc ttgttgtatt cacgcaggaa ggggagtgat ttccagacat
     6241 tttggttgtc gcccgccagt ttcacaccgc tatttttatc agtgccggtg gcaatcagaa
     6301 catagtggaa gccctgattt tttaactgtt caacggttaa atcgggtgag cagccatact
     6361 caaatttcac gccgtgatcg gcaacaaaat cgatatcgtg ctgaattaac tctgcaggaa
     6421 tacggaactg aggaatgata tttttcacta cgccgcccgc attggcttcg cgttcaaaca
     6481 gcgtaaccgg atggcccgct ctggcaagga agtaacctgc agccagaccc gccggacctg
     6541 caccaatcac ggcaaccgga tggcgtgaac cagaaccggc tggtttgtgc cagcgttgct
     6601 tatattcatc ccaacctttt tccagcgcga cttttttcag ttcgcggata ttcagcgcac
     6661 tgtcgtaatc caggcgggta cagttgtatt ggcactggtg atcgcaaata tgaccggtaa
     6721 tggcaggtag ggcgttgcgt tggtagatga gttccagcgc gtcggcatag cggtgttcgc
     6781 caagcagacg gatgtattcc ggaatatctt gcttgatagc gcaggcagta acacaggggg
     6841 caacgtagca gtcggtcagc ggcaggtctt ctgccacttc aatacgctct tctggcttcc
     6901 agtgtttctg ggtgtattcc atggttaacg cgtctgctgc cagtctgttc agtcgttcga
     6961 cgtcaacatg gtcaagtccc caggcgtcgg agccttccag ctcgcgcatg caggcactta
     7021 agcgcagata gccgccaggt ttcagcagat cggttgccat agtaataggg cgaataccag
     7081 tatcaaaaat atcgcggata gtcagctgac tggcaccacc ggaataagaa atgggcagtt
     7141 tgccgtcaaa ggcgcgagag agaactgctg caacattgat ggagagcggg aacagcgcac
     7201 ggcctgacat atacatctct tcaccgggca gtgcgccttt attgttgatg gtgccgagag
     7261 tgttagtcag ttttacgcca aagccgagtg atttttcttt tgccagtgcc atcaggcgtt
     7321 ccagcatttc cagtgcttgc gtcagcttga ggtcgtgatc aaatgactct tcttttaagc
     7381 ctatgtaacc gaaaccgcag acatcgagga tctcacgaac acgcgcgtac ccgagtaagg
     7441 tcgggttaag tttgacaaag gtgttgagcc ctttttcttc cagcatgtag cggcaaatgg
     7501 cttcgatttc atgcggagga cagccgtgca tggtggagag ggtgacgccc tgcaccatac
     7561 tggtggggat gcgagcgggt aaagcttgca agctttcgcg tttttcctgc aatccgtggc
     7621 gggctaaaaa tgcgtcatcc tggagtaatt tattcagcgt atcgcgatat tgcgcgaatt
     7681 tcggatggtc agatgcgtcc atcatattgt cgatgaactg ttgcatcggc ggttgcttaa
     7741 taccttcaag gttgtaaccg acgctcatat taaagatgaa cgatttaccg gaatcagaag
     7801 gctggaacat cgcttcgaga aggtgcaggg caaaccaggc tttgaggtat tcatcccagg
     7861 ctttaagcag ggtaaattcg gtagaccatt cggtgttaaa gcactcgtct tcggcatcga
     7921 tacaaggctt ttccagctcc aggcggtcaa gaatttggac ggtttttagt tcgatgaagc
     7981 gtccgccagt cagccaggaa gtgacaatat tttgcgcgag ctgcgtgtgc gggccagcgg
     8041 cagggccgac gggagtggca caggtttcac cgaagacgct aacagtttta ccttttacgg
     8101 gtgagtaaaa ctgttgctcg ggaataccaa agattgagcg ttgttgttgg tattcatcaa
     8161 atatgcgcgt caaaagttcc tcaaacggaa tgggacgcat aatatccccc ataaccctct
     8221 ccttagctta tacagatatc attgagtgac cggtgttgag aaatccggtg gtttaggggg
     8281 atagagcaac aatcgcacca ctcttgccag gtttggcggg aaatataaaa aaatgtgagg
     8341 cggctcgaaa cttaagggct ttttgtgaat gaactcacag gtgccaaatg
//
LOCUS       DAZKFU020000204         8325 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZKFU020000204 DAZKFU020000000
VERSION     DAZKFU020000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.206"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(196..1086)
                     /gene="malG"
                     /locus_tag="ACHOQQ_RS18705"
                     /old_locus_tag="pgaptmp_003741"
     CDS             complement(196..1086)
                     /gene="malG"
                     /locus_tag="ACHOQQ_RS18705"
                     /old_locus_tag="pgaptmp_003741"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGU4471946.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1101..2645)
                     /gene="malF"
                     /locus_tag="ACHOQQ_RS18710"
                     /old_locus_tag="pgaptmp_003742"
     CDS             complement(1101..2645)
                     /gene="malF"
                     /locus_tag="ACHOQQ_RS18710"
                     /old_locus_tag="pgaptmp_003742"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGU4471947.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2799..3989)
                     /gene="malE"
                     /locus_tag="ACHOQQ_RS18715"
                     /old_locus_tag="pgaptmp_003743"
     CDS             complement(2799..3989)
                     /gene="malE"
                     /locus_tag="ACHOQQ_RS18715"
                     /old_locus_tag="pgaptmp_003743"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGU4471948.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4354..5469
                     /gene="malK"
                     /locus_tag="ACHOQQ_RS18720"
                     /old_locus_tag="pgaptmp_003744"
     CDS             4354..5469
                     /gene="malK"
                     /locus_tag="ACHOQQ_RS18720"
                     /old_locus_tag="pgaptmp_003744"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGU4471949.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5541..6881
                     /gene="lamB"
                     /locus_tag="ACHOQQ_RS18725"
                     /old_locus_tag="pgaptmp_003745"
     CDS             5541..6881
                     /gene="lamB"
                     /locus_tag="ACHOQQ_RS18725"
                     /old_locus_tag="pgaptmp_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGU4471950.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7124..8044
                     /gene="malM"
                     /locus_tag="ACHOQQ_RS18730"
                     /old_locus_tag="pgaptmp_003746"
     CDS             7124..8044
                     /gene="malM"
                     /locus_tag="ACHOQQ_RS18730"
                     /old_locus_tag="pgaptmp_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGU4471951.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVAGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         2043 a         2173 c         2160 g         1949 t
ORIGIN      
        1 cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa aaataataag ttctatgatg
       61 gaaatattaa ccggcgaacg attcagattg cagacgaaag aaaaaaaggc gctccgtgga
      121 gcgccgaata acagtcacaa gttgggataa cgtaagttga gggtgcagcg gcataacatt
      181 ggcagaacaa catctttaac ctttcacacc acctgccgtc aggccgttga ccagccagcg
      241 ttgagctagc aagaagacga tggtgatcgg taatgcagac atcacggcag cggcggcaaa
      301 gtcaccccac aggtagtttt gcgggttgag gtattgctgc atccccacgg ccagggtgta
      361 gctgtttacg tcacgcagta acagcgatgc gaccggaact tcagtaatgg cagcgataaa
      421 cgacaggatg aacaccaccg ccagaatcgg tactgacagc ggcaacagga caaggcggaa
      481 ggcctgccac ggtgtcgcac catccagcgc cgccgcttct tccagcgaac tgtcgatggt
      541 ttcgaaatag cctttgatgg tccagacgtg cagcgcaatc ccacccagat acgcgaaaat
      601 tacgccgccg tgagtattca ggccaatgaa tggaatgtac tcacccagac gatcaaacaa
      661 cgcatacaac gcgaccagtg aaagtactgc cgggaacatc tggaaaatca gcattccttt
      721 cagcagcgtc gctttgcctg gaaagcgcat acgggcgaaa gcgtaggcgc aggtggtgga
      781 gagcgccaca atgccaatcg cggaaatccc ggcgaccttt accgagttcc acagccacag
      841 cagtaccggg aatggcggtg gcgtaatgcg accatcagcc tgttcaacgc taaaacctaa
      901 cgccagtttc cagtgatccc aggagatttg ctccgggatc aggctaccgg tcgcaaagtt
      961 cccctggcgc agtgagatgg cgacgaccat cagcagcggg aacataatcg ccgcgataaa
     1021 aagtagcagt agcaggtgag taataaacaa acgtgctttt tgcgatttcg gttggaccat
     1081 tgccattttt gttatctccc ttaatcaaac ttcattcgcg tggctttcag gttcactatc
     1141 gccagcgcac ctaccagcag gaagatcagc gtggcaattg ctgctgccag accgaagtcc
     1201 tgacccccgc cgccttcaaa agcgatgcgg taggtgtagt taacaagcag gtcggtataa
     1261 ccggctggcg tggtcgtgcc aagacgatcc gggccgccgt tggttaacag ttgaatcagc
     1321 acgaagttgt taaagttaaa ggcgaagctg gcgatcatca gcggcgtcag cggtttaatc
     1381 agcagcggca gcgtaatctt aaagaagttc tggaacggac ctgcaccatc cattgctgag
     1441 gcttcataca aatcgtccgg aatcgctttc agcaagccca tgcagaggat catcatgtac
     1501 ggataaccca gccaggtatt gacgataatc agcatcgttc gggcggtggt cggatcgctg
     1561 aaccaggcgg gcttcacgcc aaacagcgcg ctcaacatca tgttgatctc accgaagctc
     1621 tggttaaaca accctttgaa aatcaagatt gaaatgaacg atggcaccgc gtagggcaga
     1681 atcagcagga cgcgatagac cgctttgccg cgcaacgctt cccactgcac cagacacgcc
     1741 agaaccatgc cgactgccac tgttaaaaag acagtgatca gcgagaacac cacggtccag
     1801 acgaaaatgg cgaggaacgg tttctgaatg ccttcgtcgg tgaagacgcg ggtaaagttt
     1861 ttccagccag tagtcacggt gtaaccgggg cttagctttt catcgcccca gttgccgtcg
     1921 gcggtaatgg actggtaaaa gccaatttgg ttattcggac gatatttcac gccgctctga
     1981 ttattcgtca acgtgccgtc accgtcgagt gtgtagagcg gctgcgtgcc agaaaactgg
     2041 cgcagggagc tcatcatcac tttgttgcca tccggcagaa tggcggtaat gtcactcagc
     2101 gcctgacgat tctgggtaat cacgcgtaga ttcgcgcgtt cgccttcggg ctgggcggtc
     2161 gtttctttca gttgcagttt ttgctctccg ccaaatttaa aagcgtcgga gaggtaattt
     2221 ttgccggttt cgccgtcgct gagcgccagt tgccactcat cgcccgccgg gtaaagacca
     2281 aagttatagg ttttgcctgc ttgccaggag cgatctaaca acacttcctg tgcacgttca
     2341 aaagtcagct ggttagtgct gctgtagttt gtgaaggcaa tggcgatggt gcagaccaga
     2401 gggaagagga cgaataatcc cattccagcc attcccgggt aaacatagcg ccaggcgtag
     2461 gctttacgat tggcgaaaat atacagcccc gctgaactca atatcagcgt ggtaatggcg
     2521 aacaggtatt ccccttgtgc gtacattaaa acaacaaggt aacccaccag caggccgagc
     2581 agacctaaca ctgaccattt cagcgcgtcg ctttgccacc aatgtttctt tttaatgaca
     2641 tccatggggt tcttcctcat tccaggacgg ataaggcttt cacgccttat ccgacaacaa
     2701 ctgcctgatg cgacgctgac gcgtcttatc aggcctacat acgtttcggt tttgtaggcc
     2761 ggacaaggcg ttcacgccgc atccggcatt tcacagcatt acttggtgat acgagtctgc
     2821 gcgtctttca gggcttcatc gacagtctga cgaccgctgg cggcgttgat caccgcagta
     2881 cgcactgcat accagaaagc ggacatctgc gggatgttcg gcatgatttc acctttctgg
     2941 gcgttttcca tggtggctgc aatacgtgga tctttcgcca actcttcctc gtaagacttc
     3001 agcgctacgg cacccagcgg tttgtcttta ttaaccgctt ccagaccttc gtcagtcagc
     3061 agatagtttt cgaggaactc tttcgccagc tctttgttcg gactggcggc gttaatacct
     3121 gcgctcagca cgccaacgaa cggcttggat ggttgaccct tgaaggtcgg cagtaccgtt
     3181 acaccataat tcactttact ggtgtcgatg ttggaccatg cccacgggcc gttgatagtc
     3241 atcgctgttt cgcctttatt aaaggcagct tctgcgatgg agtaatcggt gtctgcattc
     3301 atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac ccgctttcgc gccagcgtta
     3361 tccacgccca cgtctttaat gtcgtacttg ccgttttcat acttgaacgc ataacccccg
     3421 tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca ggttgaacat cagcgcgctc
     3481 ttacctttcg ctttcagttc tttatccagc gccgggatct cttcccaggt ttttggcggg
     3541 ttcggcagca gatctttgtt ataaatcagc gataacgctt caacagcgat cgggtaagca
     3601 atcagcttgc cgttgtaacg tacggcatcc caggtaaacg gatacagctt gtcctggaac
     3661 gctttgtccg gggtgatttc agccaacagg ccagattgag cgtagccacc aaagcggtcg
     3721 tgtgcccaga agataatgtc agggccatcg ccagttgccg caacctgagg gaatttctct
     3781 tccagtttat ccggatgctc aacggtgact ttaattccgg tatctttctc gaatttctta
     3841 ccgacttcag cgagaccgtt atagccttta tcgccgttaa tccagattac cagtttacct
     3901 tcttcgattt tggcgagagc cgaggcggaa aacatcatcg tcgttaatgc ggataatgcg
     3961 aggatgcgtg cacctgtttt tattttcata atctatggtc cttgttggtg aagtgctcgt
     4021 gaaaacacct aaacggactc tagtttcttt atacggcaac ctctttccat cctccttgcc
     4081 cctacgcccc accgttgctt tgtgtgatct ctgttacaga attggcggta atgtggagat
     4141 gcgcacataa aatcgccacg atttttgcaa gcaacatcac gaaattcctt acatgacctc
     4201 ggtttagttc acagaagccg cgttctcatc ctcccgcctc ctcccccata aaaaagccag
     4261 ggggtggagg atttaagcca tctcctgatg acgcatagtc agcccatcat gaatgttgct
     4321 gtcgatgaca ggttgttaca aagggagaag ggcatggcga gcgtacagct gcaaaatgta
     4381 acgaaagcct ggggtgaggt cgtggtatcg aaagatatca atctcgatat ccatgaaggt
     4441 gaatttgtgg tgtttgtcgg accgtctggc tgcggtaaat cgactttact gcgcatgatt
     4501 gccgggcttg agacgatcac cagcggcgac ttgttcatcg gtgagaaacg gatgaatgac
     4561 actccgccag cagaacgcgg cgttggtatg gtgtttcagt cttacgcgct ctatccccac
     4621 ctgtcagtag cagaaaacat gtcatttggc ctgaaactgg ctggcgcaaa aaaagaggtg
     4681 attaaccaac gcgtcaacca ggtggcggaa gtgctgcaac tggcgcattt gctggatcgc
     4741 aaaccgaaag cgctctccgg tggtcagcgt cagcgcgtgg cgattggccg tacgctggtg
     4801 gccgagccaa gcgtattttt gctcgatgaa ccgctctcca acctcgatgc tgcactgcgt
     4861 gtgcaaatgc gtatcgaaat ctcccgtctg cataaacgcc tgggccgcac aatgatttac
     4921 gtcacccacg atcaggtcga agcgatgacg ctggccgaca aaatcgtggt gctggacgcc
     4981 ggtcgcgtgg cgcaggttgg gaaaccgctg gagctgtacc actatccggc agaccgtttt
     5041 gtcgccggat ttatcggttc gccaaagatg aacttcctgc cggtaaaagt gaccgccacc
     5101 gcaatcgatc aagtgcaggt ggagctgccg atgccaaatc gtcagcaagt ctggctgcca
     5161 gttgaaagcc gtgatgtcca ggttggagcc aatatgtcgc tgggtattcg cccggaacat
     5221 ctactgccga gtgatatcgc tgacgtcatc cttgagggtg aagttcaggt cgtcgagcaa
     5281 ctcggcaacg aaacccaaat ccatatccag atcccttcca ttcgtcaaaa cctggtgtac
     5341 cgccagaacg acgtggtgtt ggtagaagaa ggtgccacat tcgctatcgg cctgccgcca
     5401 gagcgttgcc atctgttccg tgaggatggc actgcatgtc gtcgactgca taaggagccg
     5461 ggcgtttaag caccccacaa aacacacaaa gcctgtcaca ggtgatgtga aaaaagaaaa
     5521 gcaatgactc aggagataga atgatgatta ctctgcgcaa acttcctctg gcggttgccg
     5581 tcgcagcggg cgtaatgtct gctcaggcaa tggctgttga tttccacggc tatgcacgtt
     5641 ccggtattgg ctggacaggt agcggcggtg aacaacagtg tttccagact accggtgctc
     5701 aaagtaaata ccgtcttggc aacgaatgtg aaacttatgc tgaattaaaa ttgggtcagg
     5761 aagtgtggaa agagggcgat aagagcttct atttcgacac taacgtggcc tattccgtcg
     5821 cgcaacagaa tgactgggaa gctaccgacc cggccttccg tgaagcaaac gtgcagggta
     5881 aaaacctgat cgaatggctg ccaggctcca ccatctgggc aggtaagcgc ttctaccaac
     5941 gtcatgacgt tcatatgatc gacttctact actgggatat ttctggtcct ggtgccggtc
     6001 tggaaaacat cgatgttggc ttcggtaaac tctctctggc agcaacccgc tcctctgaag
     6061 caggtggttc ttcctctttc gccagcaaca atatttatga ctataccaac gaaaccgcga
     6121 acgacgtttt cgatgtgcgt ttagcgcaga tggaaatcaa cccgggcggc acattagaac
     6181 tgggtgtcga ctacggtcgt gccaacctgc gtgataacta tcgtctggtt gatggcgcat
     6241 cgaaagacgg ctggttattc actgctgaac atactcagag tgtcctgaag ggctttaaca
     6301 agtttgttgt tcagtacgct actgactcga tgacctcgca gggtaaaggt ctgtcgcagg
     6361 gttctggcgt tgcatttgat aacgaaaaat ttgcctacaa tatcaacaac aacggtcaca
     6421 tgctgcgtat cctcgaccac ggtgcgatct ccatgggcga caactgggac atgatgtacg
     6481 tgggtatgta ccaggatatc aactgggata acgacaacgg caccaagtgg tggaccgtcg
     6541 gtattcgccc gatgtacaag tggacgccaa tcatgagcac cgtgatggaa atcggctacg
     6601 acaacgtcga atcccagcgc accggcgaca agaacaatca gtacaaaatt accctcgcac
     6661 aacaatggca ggctggcgac agcatctggt cacgcccggc tattcgtgtc ttcgcaacct
     6721 acgccaagtg ggatgagaaa tggggttacg actacaacgg cgatagcaag gttaacccga
     6781 actacggcaa agccgttcct gctgatttca acggcggcag cttcggtcgt ggcgacagcg
     6841 acgagtggac cttcggtgcc cagatggaaa tctggtggta atagcaaaac ctgggccgga
     6901 taaggtgctt gcgccgcatc cggcatccaa cgcctgatgc gacgcttgcg cgtcttatca
     6961 ggcctacaac ggctgtcaaa tgtaggccgg ataaggcgtt tacgccgcat ccggcataaa
     7021 aacaggttgt cattatctga aaggggcgca agcccctctg attatcgggt ttagcgcgct
     7081 attgcctggc taccgctgag ctccagattt tgaggtgaaa acaatgaaaa tgaataaaag
     7141 tctcatcgcc ctctgtttat cagcagggtt gctggcaagc gcgcctggaa tcagccttgc
     7201 cgatgttaac tacgtaccgc aaaacaccag cgacgcgcca gccattccat ctgccgcgct
     7261 gcaacaactc acctggacac cggtcgatca atctaaaacc cagaccaccc aactggcgac
     7321 tggcggccaa caactgaacg ttgctggcat cagtggtccg gttgctgcgt acagcgtccc
     7381 ggcaaacatt ggcgaactga ccctgacgct gaccagcgaa gtgaacaaac aaaccagcgt
     7441 atttgcgccg aacgtgctga ttcttgatca gaacatgacc ccatcagcct tctttcccag
     7501 cagttatttc acctaccagg aaccaggcgt gatgagtgca gatcggctgg aaggcgttat
     7561 gcgcctgaca ccggcgttgg ggcagcaaaa actttatgtt ctggtcttta ccacggaaaa
     7621 agatctccag cagacgaccc aactgctcga cccggctaaa gcctatgcca aaggcgttgg
     7681 taactcgatc ccggatattc ccgatccggt tgctcgtcat accaccgatg gcttactgaa
     7741 actgaaagtg aaaacgaact ccagctccag cgtgttggtc gggcctttat ttggttcttc
     7801 cgctccagct ccggttacgg taggcaacac ggcggcacca gctgtggctg cacccgctcc
     7861 ggtgccggtg aagaaaagcg agccgatgct caacgacacg gaaagttatt ttaataccgc
     7921 gatcaaaaac gctgtcgcga aaggtgatgt tgataaggcg ttaaaactgc ttgatgaagc
     7981 tgaacgcctg ggatcgacat ctgcccgttc cacctttatc agcagtgtaa aaggcaaggg
     8041 gtaattacgc cccacagtgc tgattttgca acaactggtg cgtctcctgg cgcacctttt
     8101 tttatgcttc attcctggga tatgagcgat tttttatagt aactcacttc ttcttcacta
     8161 agaatatcca ttatctcaat gccttatcag agattctttt cctttcgccg gtagtgtctg
     8221 gacattcagg ctacttttcc aggttatttc atttctgtaa tgcagaggtt ttatgttagg
     8281 tcatatctca aagtttgacg gcaataactc tttgataaaa catgg
//
LOCUS       DAZKFU020000205         8290 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZKFU020000205 DAZKFU020000000
VERSION     DAZKFU020000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.207"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..7975)
                     /locus_tag="ACHOQQ_RS18735"
                     /old_locus_tag="pgaptmp_003747"
     CDS             complement(<1..7975)
                     /locus_tag="ACHOQQ_RS18735"
                     /old_locus_tag="pgaptmp_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU4471952.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLVTQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALKAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSTQTLSADSHSSATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQVLTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPPGEITVTDTAPQQLTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGIAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVYTAEATLPNGNNDTKMVNIAPDASNALVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     VEATLLNGNGYTTTVNIAPDASNAQVTLNIPAQQVVTNNSDNVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTAVPDRIIAGTPQNSSGSVITATVVDNNGFPVKGVTVSFTSRTKSAEMTNGGQAVTN
                     EQGKATVTYTNTRSSRETGARPDTIEASLENGSSTLSTSIQVDADASTAHLTSLYTLY
                     DTQLAGDDTTLYITVNDNYGNGVPLHQVTLSVSPSEGVTLSNNGINTTNHDGYLYASM
                     TATKAGVYQVTATLDNGDSMQHTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVRANFTLSDGGKAITDTEGKAKVTLKGTKAGAHTVTAS
                     MAGGKSGQLVVNFTADTLTAQVNLNVTEDNFIANNIGMTKLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNSYGVSDTKPVT
                     LIADAGTAKLAGFTASSGSFTASTTEGATLTASVTDAYGNPLEGIKVNFRGPATTLSN
                     TSVETDAQGKAEILVTSTIAGTKVVTANLAIAPTEAAMRTLTVKADIDSATITSLEMP
                     EGQVIIREPIAVKAHVDDQFGNPVADQLVTFSAEPSSFNMVISQDTVSTNRQGIAEVT
                     MTPGRYGSYTVKASLANGSFYEKDLVVIDLRLTLTSSSQLIGVNDPSGATLTVRLTHA
                     NGAPLSHELVTFSVTPEGATLSSQTATTNTSGEAQVVLTSNKVGTYAVTASIHSGVII
                     ETQTTVKVTGNPSTAHVASFIADPSTIPANNSDISTLKATVEDGSGNLVEGVNVNFVL
                     KSGSATLTSLTAVTDQNGLATTSVRGAMTGSVTVSAETSYGGAQTVDITLVAGPADAS
                     LSVLKNNRSSLKGDFTESAELHLVLHDLSGHPINVSEGMEFVQSGTNVPYVQVSAIDY
                     SKNFSGEYKATVTGGGEGIATLIPVLNGVHQAGLNTTIEFISAEARPMTGTVSVNGAT
                     LPAASFPSQGFTGAYYQLNNDNFAPGKTAADYAFSSTASWVDVDTSGKVTFKNVGDRN
                     AVIITATPRSGGAIYQTQVRVKGWWVNHGNNLMQLSQAENYCSNQVGNGYTLPRADLL
                     SNGHMRREIGSLYGEWGDMGNYMNEADFYSMVYWSSNSAGAGQQYIVSLETGTQNTYQ
                     THEF"
BASE COUNT         1783 a         2091 c         2077 g         2339 t
ORIGIN      
        1 aaaattcatg cgtttgataa gtgttttgcg tccctgtttc cagggagaca atgtattgtt
       61 ggcctgcgcc tgcagaatta gatgaccaat ataccattga ataaaaatca gcttcgttca
      121 tgtaatttcc catatctccc cattcaccat acagtgaccc tatttcgcgc cgcatgtgtc
      181 cattgcttaa aaggtccgcg cggggaaggg tgtagccatt accgacctgg ttgctgcagt
      241 agttttcagc ctggcttaat tgcatgaggt tattaccgtg attcacccac cagcccttca
      301 cccgtacttg cgtttggtaa atagcgccac ctgatcttgg ggtcgccgtg attattacag
      361 catttctatc tccgacattt ttgaatgtca ccttaccgga agtatcaaca tcaacccagg
      421 aggcagtgct tgaaaacgca taatcagccg ccgtttttcc tggtgcaaag ttgtcattat
      481 tcaactgata atacgccccg gtaaatccct gcgaagggaa tgaagctgcc ggtagggtcg
      541 caccattgac tgacactgta ccggtcattg gtctggcttc cgcgctaatg aattctatcg
      601 tggtattcag acctgcctgg tgaacaccat tcaacacggg gatcagcgtt gcgataccct
      661 ctccgccgcc tgtaacagtg gctttgtact cgccggagaa atttttgctg tagtcaattg
      721 cactaacttg cacatagggc acgttggtac ctgattgcac aaattccatc ccttcagaaa
      781 cattgatcgg atggcctgat agatcgtgca gaacaagatg tagttcagca ctttcggtaa
      841 agtccccttt cagtgatgac cggttgttct taaggacgga cagcgaagcg tctgccgggc
      901 ctgccaccag cgttatatct actgtttgcg ctccaccata gctcgtttct gcgcttaccg
      961 tgacgctccc cgtcatagct cctctaacgc tcgttgtcgc gagtccgttt tgatcggtca
     1021 ccgctgttaa tgacgtcaat gtggcagagc cgctttttaa gacgaagttt acattgaccc
     1081 cttcgaccag gttaccactg ccatcctcaa cagtagcctt tagcgtgctg atatcactat
     1141 tgttggccgg gatagtcgac ggatcagcga taaaactagc aacatgtgcg gtgcttgggt
     1201 tgccagtaac cttcacggtt gtctgtgtct caataattac gccgctatgg atcgatgctg
     1261 tcaccgcata tgtaccgact ttattgctgg tcagaacgac ttgtgcctca cctgaagtgt
     1321 tggttgtcgc tgtttggcta cttaacgttg caccttcggg cgttacgcta aaggtaacga
     1381 gttcatggct caatggagcc ccgtttgcat gagttaacct tacagtcaga gttgctcccg
     1441 atgggtcatt gacgccgata agttgacttg atgacgtgag tgttagtctt agatcgatta
     1501 ccaccaggtc cttctcataa aaggagccat tcgccaggga tgctttcaca gtatacgaac
     1561 cgtatcttcc aggcgtcatg gtgacctcag ctataccctg cctattcgta gaaaccgtat
     1621 cctgactaat gaccatgtta aaacttgacg gctctgcact gaatgtcaca agttggtcag
     1681 caactggatt gccaaactgg tcgtcgacgt gtgctttaac cgctatgggt tctctgatta
     1741 ttacctgacc ttcaggcatt tccagactgg tgatcgtcgc agaatcaata tctgctttga
     1801 cagtcagcgt tcgcatggcg gcttcggtag gtgcgattgc caaattagct gtaactactt
     1861 tcgttccagc aatggtgctt gtcacaagga tttctgcctt accctgagca tccgtttcga
     1921 cactggtgtt actcagcgtg gtggcggggc cgcggaaatt gaccttgata ccttctagcg
     1981 ggttgccgta agcgtcagtg acgcttgccg tcagggtcgc gccctcggtt gtgctcgcgg
     2041 taaatgagcc cgatgatgcg gtaaaaccag ccagtttagc ggtaccagca tcggcaatca
     2101 aagtcaccgg cttcgtatca ctgacaccgt aactgttcac gctaactgtc acgggatagg
     2161 tgccggattt tgtaccgctc agtgtaactt cagccttgcc gttgatgtct gtaatggcgg
     2221 aaccgccttg tccgagagtg aagcttgcgc tcacatctgc cggtagtgtg aatgttaccg
     2281 cctcattggc taacgggttg ccgtttccat cagtcactgt tgcctgaagt ttggtcatcc
     2341 caatgttatt ggcgatgaaa ttgtcctcgg taacgttaag attaacctgc gcagtgagcg
     2401 tatccgcagt aaagttcacc accagttgtc cgctcttacc gccagccatc gatgctgtaa
     2461 cagtatgagc gcctgctttt gtacctttca gcgtgacttt cgctttgcct tcagtatcag
     2521 taatcgcttt accgccatcg ctcagtgtga agttcgccct cacatcttcc ggcagagtaa
     2581 atgttacctc agtgttggct atcgcattgc cctctgtatc agcgactgtt gctgttagtg
     2641 tcgtgagatc gttattgtcg gcaatcaccg gatccttcga ggctgccagc gtgatttcag
     2701 cattcgcgac gttcggcaca taggtcactg tgtgttgcat cgaatcgcca ttgtcgaggg
     2761 ttgccgtcac ttgataaacc ccggcttttg tagcggtcat gcttgcgtat aaatagccgt
     2821 cgtggttggt agtatttata ccgttattac tgagggtcac gccttcactc ggcgaaacgc
     2881 tgagggttac ctgatgcagg ggtacaccat tgccgtaatt atcattcacc gtaatgtaca
     2941 gagtggtatc gtcgcctgcc aattgtgtgt cataaagtgt atatagcgag gtgagatgtg
     3001 ccgtagacgc atcagcgtcg acttgaatgg acgtgctaag cgtggagcta ccattttcca
     3061 ggctagcttc aatggtatcc ggtctcgctc ctgtttctct cgaggagcgg gtattggtat
     3121 aagtgacggt agctttaccc tgttcgttcg tcacggcttg gccgccgttc gtcatttcgg
     3181 cagattttgt tctgctggta aagctcacag tcacaccttt taccggaaag ccattattat
     3241 caacgactgt ggcggtaatg acgctgccgg agctgttctg cggggtaccg gcgattatgc
     3301 ggtctggcac agccgtcaac tcgataattt ttgccgccgc tgtgtcgcca ataaaatgca
     3361 cagttttgtt gtcgctggca ccattattag ccagtgatgc agtgaccgtc tgctcaccaa
     3421 aggcaacgcc tgcgagagtg gcctgcgcga tgccagactc attggtatta ctcactcccg
     3481 gggtcagggt gagtcctgaa gaggctgagc tgaatgttac cggaagattg ttcacctcat
     3541 tgtcgaactg atctttaacc gttgcagtta gcgttgcgtt atcgacgcca ttacctgtga
     3601 tctcatcttt tgacatctgc aggacaacct gagccgaggt tttgtccgcc acaaacgtta
     3661 ccggctgcga atcactggta ttgttattac ccagcgttgc ggtaaccgta tgcgtgcccg
     3721 cttttttacc tttcagcgtg acatgcgctt ccccattggc ctgggtaatg gcaatgccgt
     3781 tattttcaag ggtaaagttt gctgccacgt cctgtggcat ggtgaagttt accgttatcc
     3841 ccgctaccgg atgattcgat gggtctttca ccgtcgccgt cagctgcaca ttgtcgctgt
     3901 tattcgtcac cacctgttga gcagggatgt tcagggtgac ctgcgcgttg ctggcatcgg
     3961 gcgcaatatt gactgtcgtc gtatagccat tgccgttaag cagtgtggct tcgactgtat
     4021 gaacacccaa aaccgtgccc ttaagggtaa cttcagcaac accactgtcg ttagtattgc
     4081 ttgtgctctg actaagttga gtatctgccg ggttggtact gaaggtgacc tgtagatctt
     4141 ttactgcgtt atcaaaagga tctttcactg ttgctgtcag agtcgtctca tccacgccat
     4201 tcccaatgat ttccgctttc gatgtttgca gaacgacaac tgcgctgtcc ttatccgcca
     4261 cgaatgtgac tggttgcgca tcgctggcat tattgttacc cagcgttgcg gtaaccgtat
     4321 gcgtgcccgc ttttttgcct ttgagggtga cgtgcgcttc gccattggcc tgagtgatgg
     4381 caataccgtt attttcaagg gtaaagtttg ccgccacgtc ctgtggcatg gtgaagttca
     4441 ccgttattcc cgccaccgga tgattcgacg gatctttcac cgtcgccgtc agctgcacgc
     4501 tgtcgctgtt attcgtcacc acctgttgag ccgggatgtt cagggtgacc agcgcgttgc
     4561 tggcatcggg cgcaatattg accatcttcg tatcgttgtt gccgttaggc agtgtggctt
     4621 cggctgtgta aacccccaaa accgtgccct taagggtaac ttcagcaata ccgttctcat
     4681 tagtattacg cgcgttcaga ctcagttgcg tgtctgcggg ggaggtgcgg aagactactg
     4741 acagattttt aaccacgtta tcaaaaggat ctttcactgt tgctgtcaga gtcgtctcat
     4801 ccacgccatt cccaatgatt tccgctttcg atgtttgcag aacgacaacc gctctgtctt
     4861 tatccgccac aaacgtcatt ggctgtgtat cgctgacatt gctgttagcc agacgagccg
     4921 tgatcatatg cgtgcccgct aacgtgccgg tcagggaggc gattgcaatc ccgttgctat
     4981 ccgtcatgcc ttttccgctg ttgctaatcg agaaccgact tgcgacgtcg tttggcacag
     5041 agaaagtgat ttctttatct tttagtgggt tgccattttt atcctgcaag gttgccgtca
     5101 gctgttgagg cgctgtgtcg gtgacggtga tctcgcctgg gggcacactg agggtcaggg
     5161 ctgcagtact ttgatcaccg ataaaaatca cctgttgatt agcctgagaa ccagagctca
     5221 cagaggccgt aaccgtataa tcaccatttt tcaaactggt cagcgtagct gtggcgatcc
     5281 cgtcgtggct attcacttcg gtttggctca gtttcgctgc tgctgaatta acattgaagg
     5341 tgaccttgac gtcattgagc aggttgcctt ttgcatcccg gactgtcgcg gtcattgtgg
     5401 cgctgtcatt gccgtcagcg accacttcat ttttcgactt ctgcagatca acctgcgcgg
     5461 tactcgagtc gccgacaaaa ctgacaatta atgtttgttt cacgccattt tcaagggtga
     5521 cttcaaccgt gttgtcttcc tgcttactac ttttcagatc aaaagtcgcc agaccattaa
     5581 catccgtttt tgcggtgttt tgattgttaa aggaagttgc cgatccgctt aataccgcaa
     5641 acgtgacggt atgatcattg attgggttgc ttccttcatc agcgacattg accgagacgg
     5701 tgtttgctgc attctcattg gcgagcacac cattattgct ggcagataat gtcgcaattt
     5761 tcgctgactg cgggttggcg tcgatgataa atccagcggt atgcaaatct tcattccagt
     5821 tttgcattaa cagcttcgca gtaagcccac tgcctttggt ataggcggta taggttgcct
     5881 tatagacgcc atctgcggtt tctttccagt ctgtagtgac accaggtttc acattgtcga
     5941 tgctgactgc ggtattcagt tgctgttttt gctccttaac aggtttgtca ttttcatctc
     6001 tcagttctac cgtcacctcg ataggattcc cggagagata acggtcctta tcaatcttaa
     6061 ttgacgagtg agtccgggat gacgaaacag aaatgatatt caccacggcg ggggctttag
     6121 ccgcatccac accgttcagc tgtggcatca gcgtcagcgt gccagacatc gctcctgtgg
     6181 tcaggacttg ggtatagctt ccgtcaccat tatctttcca gtccgacagg gtgatgtcct
     6241 gaacaccttc gtgacgcgtc gagagcacca gcccgataac aggattacct gctgcatcat
     6301 gagcaataaa agtcagtgtg gcgcttgaat gggaatccgc gctcaacgtc tgggtactta
     6361 acgataccga ggaatctttc tggcttagcg taggtgcctg aacgactacc atgctctgtt
     6421 cacgattcga aaaattgcct ttgacatctt cagcggtgac ttcaatcggc caggtgttat
     6481 cggtttctgg cgtactggtg aaccggtacg ccggcagggt aaccagaata tctttacccg
     6541 ttgtaaccac tttaccaccg gcagctttca gagcggtggc ttcgacgtta tagcctttca
     6601 gggcatattt ggtttgtagc gacgaaacca gtgatttcac ttctcctgac ttccctgtca
     6661 cggggtctgt cagggtcagg cggaccagtt cttttttgcg atattccaga acgatgttgt
     6721 tgttgcgatc caccagatca taacggctgc ctgcaaggct acgccgtgca gcgacttcat
     6781 tcgggtcaag ctgtttctgc attgcgctgc caggttgcca ggtaaaatcg acggcaaatc
     6841 gggtgtcatt ttcgccctgt ttaccctggc gttgctccgc gctaaaggtc atcagcggga
     6901 agggggtata gtttagtcca gcggttatgg catgaggatt actttgccga tcgtctttat
     6961 cgaacagggc cacttcatcg ccataatact gttcatagac cagtttaccg ccaaggtgcg
     7021 gccaggcggg taaccagcct tctgcgcgta catcccagcc attggccggg cgtgcttcat
     7081 aatcgttgtc cagttcaggt gcgctgcgcc agtttgtcag tcgcaaatag ccgttactgc
     7141 ttaatttaag atagtcgcgc cagtactccg cgccaatgcc ggcgcgggag tggtaacggc
     7201 taagatcgtg gtcgaaaaag aagttgatgc ccgacatcca tgtgggagtg aaatgacgcc
     7261 aacccaagcc gttgttaatc tgcgtacgct cgtcagtacg atggagagta tgctgactga
     7321 aaaagagatt atcaggtgtt tcataccacg gatggagaaa atcgaactgg gagttcttca
     7381 ggctaaaatc ttcatccacg cccagcgtga ttcttgcggt accgaagcgg cttaaccagt
     7441 ctgtcattgc gcctgaagcc tgagaagagg cccatccacg cgccatattt gccgcttgct
     7501 cgctgttcat atcttcggcg agcagagatc cgatttgctg tgaagtactg gctatctgtt
     7561 gctcaaggtt gccactgcta ttacccggtg gcggggttaa attattttca ctaacttgtg
     7621 ccgggacatc cagttcatca ccctggcgga cattatcaaa acctcgagca aacgtacgaa
     7681 actggttgag tttgcgtaac tcagccaccg aaataccgaa acgttcggca acgctttggg
     7741 ccgattccag cgctccaagg gtgtagggca ccgtattggc atttgcaatg gcaatttgtg
     7801 caggaactgg ttgttgggtt gcggcgttta ccacaccttg tgctgccgca gccataggga
     7861 acacaagttg agttaccagg cagatacccg cagttaagcg gcgtagtttg atacccatcc
     7921 cgcataagat ttgtcggtca tttatttctt ctccacttct cttcttcgta gccatgctta
     7981 ccttccctga acgagctaac ataaatgcgc cctttctagc acagcgaaaa aatgtaaatc
     8041 ctgatagcaa tatgattgaa aattaataac ttttcgttag gcagttttgg gtgtgagttg
     8101 caagagggga gactactgaa ttactcaagt tttataatcg aggggaaaat ggtgatggcg
     8161 ccatagcaaa acgccctcaa ccataaaggt cgagggcgct taagatgtca aaacccgcta
     8221 tccgttaaaa aacaatgttc aactaaggtc agtgacattg cgctaaaaaa gcgaattttt
     8281 agaatttggc
//
LOCUS       DAZKFU020000206         8277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZKFU020000206 DAZKFU020000000
VERSION     DAZKFU020000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.208"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..529
                     /gene="trmL"
                     /locus_tag="ACHOQQ_RS18740"
                     /old_locus_tag="pgaptmp_003748"
     CDS             56..529
                     /gene="trmL"
                     /locus_tag="ACHOQQ_RS18740"
                     /old_locus_tag="pgaptmp_003748"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGU4471953.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYSAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(582..1403)
                     /gene="cysE"
                     /locus_tag="ACHOQQ_RS18745"
                     /old_locus_tag="pgaptmp_003749"
     CDS             complement(582..1403)
                     /gene="cysE"
                     /locus_tag="ACHOQQ_RS18745"
                     /old_locus_tag="pgaptmp_003749"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGU4471954.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKTSGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(1483..2502)
                     /gene="gpsA"
                     /locus_tag="ACHOQQ_RS18750"
                     /old_locus_tag="pgaptmp_003750"
     CDS             complement(1483..2502)
                     /gene="gpsA"
                     /locus_tag="ACHOQQ_RS18750"
                     /old_locus_tag="pgaptmp_003750"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGU4471955.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(2502..2969)
                     /gene="secB"
                     /locus_tag="ACHOQQ_RS18755"
                     /old_locus_tag="pgaptmp_003751"
     CDS             complement(2502..2969)
                     /gene="secB"
                     /locus_tag="ACHOQQ_RS18755"
                     /old_locus_tag="pgaptmp_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGU4471956.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(3032..3283)
                     /gene="grxC"
                     /locus_tag="ACHOQQ_RS18760"
                     /old_locus_tag="pgaptmp_003752"
     CDS             complement(3032..3283)
                     /gene="grxC"
                     /locus_tag="ACHOQQ_RS18760"
                     /old_locus_tag="pgaptmp_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGU4471957.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(3424..3855)
                     /locus_tag="ACHOQQ_RS18765"
                     /old_locus_tag="pgaptmp_003753"
     CDS             complement(3424..3855)
                     /locus_tag="ACHOQQ_RS18765"
                     /old_locus_tag="pgaptmp_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU4471958.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            4100..5644
                     /gene="gpmM"
                     /locus_tag="ACHOQQ_RS18770"
                     /old_locus_tag="pgaptmp_003754"
                     /gene_synonym="pgmI"
     CDS             4100..5644
                     /gene="gpmM"
                     /locus_tag="ACHOQQ_RS18770"
                     /old_locus_tag="pgaptmp_003754"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGU4471959.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKNRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVDEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            5678..6937
                     /gene="envC"
                     /locus_tag="ACHOQQ_RS18775"
                     /old_locus_tag="pgaptmp_003755"
     CDS             5678..6937
                     /gene="envC"
                     /locus_tag="ACHOQQ_RS18775"
                     /old_locus_tag="pgaptmp_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGU4471960.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            6941..7900
                     /locus_tag="ACHOQQ_RS18780"
                     /old_locus_tag="pgaptmp_003756"
     CDS             6941..7900
                     /locus_tag="ACHOQQ_RS18780"
                     /old_locus_tag="pgaptmp_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGU4471961.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPNAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(7887..>8277)
                     /gene="waaH"
                     /locus_tag="ACHOQQ_RS18785"
                     /old_locus_tag="pgaptmp_003757"
     CDS             complement(7887..>8277)
                     /gene="waaH"
                     /locus_tag="ACHOQQ_RS18785"
                     /old_locus_tag="pgaptmp_003757"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGU4471962.1"
                     /translation="YLHNTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITI
                     YPEFHQQITYEALRVCHAVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQAL
                     LWSFRLWQWRDKTRSHHRITRSAFNLR"
BASE COUNT         2033 a         2211 c         2196 g         1837 t
ORIGIN      
        1 aggccgcacg gttcaccaat tttctgctat cctgcccccg cactaagggg gcagtatgct
       61 aaacatcgta ctttacgaac cagaaattcc gccaaatact ggcaacatca tccgtctttg
      121 cgctaatacc ggctttcgtc tgcatatcat cgaaccgatg ggatttgcct gggacgataa
      181 gcgcctgcgc cgcgcggggc tggactatca cgagtttacc gccgttacgc gtcatcatga
      241 ctacagcgcg ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg
      301 tacgcctgct cacagcgccg taagctatca ggatggcgat tatctgatgt tcggcccgga
      361 aacacgcggc ctgccagcga gcattcttga tgccctgccc gctgaacaaa aaattcgcat
      421 tccgatggtg ccagacagcc gcagcatgaa cctgtccaat gcggtgtcgg tagtggtgta
      481 tgaagcctgg cggcagctgg gatatccggg agcggtgttg agagattagt tacgccatgc
      541 cggatagatg attacatcgc atccggcacg atcacaggac atcagatccc atccccatac
      601 tcaaatgtat ggttaatacc gttgaaatgc tggtccatat ccattgatgg cttatcgctg
      661 tctggtttac cgacaatgcg tgccggaacg ccagcggcgg tggtatgcgg cggcaccggt
      721 tgcagcacca cggaacctgc gccaatcttc gcgccgcgac caacttcaat attgccgaga
      781 atcttcgcgc ccgcgccaat catcacgcct tcacgaattt tcgggtgacg gtcgccgcta
      841 gttttcccag taccgccaag ggtgacggat tgcagaatcg acacgtcgtt ttcaatcacc
      901 gccgtttcac ccacaacgat acccgtggcg tggtcaagca tgataccgcg accaattttt
      961 gctgccgggt gaatatcaac ctggaacgtc acagaaacct ggttttgcag aaagattgcc
     1021 agtgcgcgac gcccctgatt ccacaaccag tgaccgatgc gataggcctg caaggcatga
     1081 aaacccttca ggtataacaa cggggttgag tatttatcga ctgccgggtc gcgggtacgc
     1141 accgcctgaa tatcacaggc cgcagaggcg atcatttccg ggtcagcggc gtaggcttct
     1201 tccaccactt cacggatagc aatagcaggc ataattggcg atgacagctt gttcgccagc
     1261 atgtagctca gtgcactgcc aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg
     1321 gccagcattg gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt gttccagaca
     1381 atttccagtt cttcacacga cattgcttac tccacacgat gagataatga ccggcccgtt
     1441 ctgcgcgggc cgggtcatag cggtaacaaa ggttccctgg ggttagtggc tgctgcgctc
     1501 gtccttgcgt gcacgaccta ataacgtcaa tgctgcctcg cgcgcgtttt ttccgcaata
     1561 taatacttga taaatttcct cggttattgg catttcaacg ccgaagcgat gcgccagttc
     1621 gcggacttct ttcgtattgc ggtagccttc caccacctga ccaatcttct cctgcgcgct
     1681 ttgtacatcc atgccctgac cgagcatcat gccaaaacgg cggttacgcg actggttgtc
     1741 ggtacaggta agcaccagat cgcccagccc cgccatgccc ataaaggtgg caggatcggc
     1801 acccagcgcc gcgccaagac gcgacatttc agccaggcca cgggtgatta gcgccgtacg
     1861 cgcattcgcg ccaaaaccga taccgtcgga catccccgcg ccaatggcaa tgacgttttt
     1921 caccgcgccg ccaagctgca cgccaatgaa atccgggtta ctgtaaacgc ggaaactttt
     1981 accgcagtgc agcagctgct gaagatcatc agcaaaagtt tggtcggtcg aggccagcga
     2041 aatggctgtc ggtaaacctg ccgccagttc tttcgcaaac gttgggccag agataaccgc
     2101 cagcggaatt tgatcgccta acgcctcgcg ggccacgtcc tgtaacagac gcccggtttc
     2161 cgcttccagc cctttggtcg cccacaccag acgcgcatca ggacgcatca gcggtttaat
     2221 ctggcgcagc acttcaccaa agacatggct gggtacgacg acgagaatat tacggctggc
     2281 tgccagcgca gtggcgagat cgctttcaag atggagtgta tcgggaaaag gcacatcggg
     2341 gagaaacgcg gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc
     2401 ccagaggaca acctcgtggc catttcttgc cagggtaatg gcaagagcgg tgccgtacga
     2461 gccggcaccg atcacagtca ttgaagcatt acgttggttc atcaggcatc ctgatgttct
     2521 tcagtacctt cgccagcctg ctgctgcaaa tagttcatga acagcgcatc gaagttaacc
     2581 ggcgcaaggt tcagttgcgg gaatgtaccg cgggatacca tgctggtgat gcactcacga
     2641 gcatacggga acagaatgtt cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct
     2701 tcgatacccg cgatggagaa aataccgccc tgctgaactt cacacaggaa cgcggtttct
     2761 tcgcccaggg aggccgttac ggtaacacgc agtaccactt cgtaaacgtc atctgccagt
     2821 tgagtagatg ccgtatccag atcaagttta acttctggct gccaatcttt ctggaaaacg
     2881 tgcggcgcgt tcggcgcttc gaaagaaata tccttggtat aaatacgttg gatctggaaa
     2941 gtcatttcgg tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa
     3001 tactgcgcaa tgtaatgcca acgttcacac gttatttcag caggggatcc agtccaccac
     3061 gtgcatccaa tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct
     3121 ggggaaccgt ggtgcgaccg ctgcgtttga tcatctcttc acgcttagcg gcgttgccat
     3181 cgatcggcaa ctcctgaaaa ctcacgccct tgctgctcag cagtgctttt gcacgatggc
     3241 aatacgggca ggtttctttg gtatagattt caacattggc cataacttag ctcctgtttt
     3301 ttaccctgaa atttcatgtt gcagggcggc agcaaaatgg agtgtcccca gaagcttata
     3361 acaataagtc gctgggggcc taattgcagc taacggcctg catcatgaaa gacgacaggt
     3421 gaattatttg ccgcgcacca aaggcaagtt ttcgccagcc cagccagcga cgccttcttt
     3481 cagcacgaat acttgcgcaa aaccagcttt cgtcagtgcg tttgcaggct cctggcactg
     3541 catgccagaa ccgtctacca cgataaccgg tttgtctttg tgcttctcaa gctcaccaac
     3601 attgttggct ttgatttcgc tcggcaacag attaatagaa cctgcgatat ggcctttacg
     3661 gaagtcatca cgctgacgta aatccacaac cacagcgtct tctttgttga tcagacgcgt
     3721 agcttcacca cgagtaatca ccttcacttt cgaggtcagg ctcttaaacg tagtcacaag
     3781 aaccgccacc agtaacgcga tccaggcgat actcagtatg ggatgacggc caacaaattg
     3841 cataatttct tgcatggggg gtaacaactc ccgacgtagt gattaaaaaa accaggaaag
     3901 gagtatacct gcgcggtgcg gcaaatacag ccagcgcgtt aactggaatg caattttgcg
     3961 gggcgcgacg aaaaaaaagc gtaaatctgg catcggcttg ccgcgagcgg tcgtatattt
     4021 tgatcttcag aggctatttt atcgattcag ctgtagtaaa attacgcaaa ttttgactct
     4081 tgagtatgag gttgtcgcaa tgtcggtttc taaaaaacct atggtactgg tgattctgga
     4141 tggctatggc tatcgcgaag aacagcagga taacgccatt tttagtgcta aaaccccggt
     4201 aatggatgca ctgtgggcca atcgtccgca taccctgatc gacgcttccg gtctggaagt
     4261 cggtctgcct gaccgtcaga tgggtaactc cgaagtaggc cacgttaacc tgggtgccgg
     4321 ccgcatcgtg tatcaggacc tgactcgtct ggacgttgaa atcaaaaatc gcgctttctt
     4381 tgctaatcca gtgctgactg gcgcggtaga taaagcgaaa aacgcaggca aagcggtaca
     4441 cattatgggt ctgctctccg caggcggcgt acacagccac gaagatcaca tcatggcaat
     4501 ggtagaactg gcagctgaac gcggcgcaga aaaaatctac ctgcacgcat tccttgacgg
     4561 tcgcgacact ccgccgcgca gtgctgaatc ctcgctgaaa aaattcgaag agaaatttgc
     4621 cgcgctgggt aaaggccgcg tagcgtctat cattggtcgt tactacgcga tggaccgcga
     4681 taaccgttgg gatcgcgttg aaaaagctta tgacctgctg actctggcgc agggtgagtt
     4741 ccaggccgat accgccgttg ctggtttgca ggctgcttat gctcgtgacg aaaacgacga
     4801 gttcgtgaaa gcgaccgtta ttcgtgcaga aggtcagcca gatgcggcaa tggaagacgg
     4861 cgatgcgctg attttcatga acttccgtgc tgaccgtgcg cgtgaaatca ctcgtgcttt
     4921 cgtgaacgct gatttcgatg gcttcgcgcg taagaaagtg gttaacgtcg atttcgtgat
     4981 gctgaccgaa tacgccgctg acatcaaaac tgcggttgct tacccacccg cttccctggt
     5041 taacaccttc ggcgagtgga tggcgaaaaa cgacaaaact cagttgcgta tttccgaaac
     5101 cgaaaaatat gcccacgtta ctttcttctt taacggtggc gtagaagagt cgttcaaagg
     5161 cgaagatcgc attctgatca actcaccgaa agtggctacc tacgatctgc aaccggaaat
     5221 gagctccgca gagctgaccg aaaaactggt tgcggcaatc aagagcggca aatacgacac
     5281 cattatctgt aactatccga acggcgacat ggtaggtcac accggggtga tggaagcagc
     5341 agttaaagcc gttgaagcgc tggatcactg cgtggatgaa gttgcgaaag cggttgagtc
     5401 tgtaggtgga caactgctga tcaccgctga ccacggtaac gctgaacaga tgcgcgatcc
     5461 ggcaacgggt caggcgcaca cagcgcacac caacctgcca gttccgctga tttacgttgg
     5521 cgataagaac gtgaaagcgg ttgaaggcgg caaactttct gacatcgcgc cgaccatgtt
     5581 gtcgctgatg ggtatggaaa tcccgcaaga gatgactggt aagccgctgt tcatcgtgga
     5641 ataatccctc cccatgaggg gaaaggcgat taataccatg acacgggccg tgaaaccgcg
     5701 caggtttgca atcaggccca tcatctacgc cagcgttctt agcgctggcg tattgttgtg
     5761 cgccttttcc gcccacgcgg atgagcgtga ccaactcaaa tctattcagg ccgatatcgc
     5821 cgcgaaagag cgcgcggtac gccaaaagca acaacaacgc gcaagcctgc tcgcacaatt
     5881 gaaaaagcag gaagaagcga tctctgaagc cacccgtaag ctgcgcgaaa cgcaaaacac
     5941 gctcaatcaa ctcaataaac agattgatga gatgaacgcg tcgattgcca aactggagca
     6001 gcaaaaagcc gcccaggagc gcagcctcgc cgcacaactg gatgccgcat tccgtcaggg
     6061 cgagcatacc ggtattcagc tgattctcag cggtgaagaa agccagcgtg gacagcgttt
     6121 acaggcttat ttcggctatc tcaaccaggc gcgacaagaa accattgccc agttgaagca
     6181 aacgcgtgaa gaagtcgcca tgcagcgtgc tgaactggaa gagaaacaga gcgagcaaca
     6241 aacgctgtta tatgagcagc gcgcccaaca ggcgaaactg actcaggcgc tgaacgagcg
     6301 taaaaagacg ctggcagggc tggagtcttc catccagcaa ggtcagcaac agttgagcga
     6361 gctgcgcgcc aacgaatccc gtctgcgtaa cagcattgcc cgtgcggaag ccgcggcgaa
     6421 agcgcgtgca gaacgagaag cacgtgaggc ccaggcggtt cgcgaccgcc agaaagaagc
     6481 gacgcgtaaa ggcaccacct acaaaccgac tgaaagcgaa aaatcgctga tgtcccgtac
     6541 cggtggtctg ggcgcgccgc gtggtcaggc attctggccg gttcgcgggc cgacgctgca
     6601 tcgctatggc gagcagctac agggtgaatt acgctggaaa gggatggtga ttggtgcctc
     6661 tgaaggtact gaagttaaag cgattgccga cggtcgggtg attctggctg actggctgca
     6721 aggctacggt ctggtggtgg tggttgagca tggtaaaggc gacatgagtc tttatggcta
     6781 taatcagagc gcactggtga gcgttggttc gcaggttcgc gcgggccagc caattgcact
     6841 ggtgggcagc agtggcggtc agggtcggcc ttcactctat ttcgaaattc gccgccaggg
     6901 tcaggcggtc aatccacagc cgtggttggg aagataagtt ttgtttccat ttcgtcgtaa
     6961 cgttcttgca tttgccgctc tgttggcgct ctcctccccc gtaattgctg gcaaacttgc
     7021 catcgtcatt gatgattttg ggtatcgccc gcacaacgaa aaccaggtgc tggcgatgcc
     7081 ttccgctatc tccgtcgctg tattacccga ttcaccgaac gccagagaga tggcgaccaa
     7141 agcgcataac agcggccacg aagtgttgat tcatctacca atggcaccat tgagtaaaca
     7201 gccgctggag aaaaatacgc tacgcccgga gatgagcagc gacgaaattg agcgcattat
     7261 tcgtagtgcg gtcaataacg tgccctatgc cgtggggatc aacaaccaca tgggcagcaa
     7321 gatgacctct aacctgtttg gtatgcagaa agtgatgcag gcgctggagc gttacaatct
     7381 ttacttcctc gacagcgtaa ccattggtaa tacccaggcg atgcgcgccg cgcaaggcac
     7441 tggcgtgaag gttattaaac ggaaggtgtt cctcgacgat tcacagaacg aagcggacat
     7501 ccgtgtgcag ttcaatcgcg caattgatct ggcgcgtcgc aacggttcaa ccattgccat
     7561 tgggcatcct cacccttcaa cggtacgcgt gctacaacag atggtttata acctgccgcc
     7621 agacattacg ctggtgaaag ccagcagctt gctgaatgaa ccgcaggttg atacgtctac
     7681 accgccgaaa aacactgtgc ccgacacacc gcgtaatccg ttccgtggcg tgaagctgtg
     7741 caaaccgaag aaaccgatag aacctgttta tgctaatcgc ttctttgaag tattaagcga
     7801 aagcatcagc cagagcacgc tgatcgttta cttccagcat cagtggcaag gctggggcaa
     7861 acagcccgaa gcggcaaaac ttaacgctag cgcaaattaa aggcgctacg cgtaatgcga
     7921 tggtgcgacc gcgttttgtc gcgccattgc cataagcgga aagaccacag taacgcctgg
     7981 taacccacct tcacgctgcg cacattggta atcaggcgct tatacatacc ggaagtaaat
     8041 atctcggcaa tcatccgttg gcgggtaaga atatccggct ctttgcgcac cgcatggcaa
     8101 acacgcaatg cttcgtaagt tatttgctga tgaaattccg gataaatcgt aattttgtcg
     8161 gcataatttc gatttaattt ctccagcagg cgggtaatct taatatagtg acgttgataa
     8221 ttaaggtttt tattcccttg tctatgcaac cgactcactg acgtattatg cagataa
//
LOCUS       DAZKFU020000207         8257 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZKFU020000207 DAZKFU020000000
VERSION     DAZKFU020000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.209"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..4034)
                     /gene="air"
                     /locus_tag="ACHOQQ_RS18790"
                     /old_locus_tag="pgaptmp_003758"
     CDS             complement(<1..4034)
                     /gene="air"
                     /locus_tag="ACHOQQ_RS18790"
                     /old_locus_tag="pgaptmp_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin EaeX/Air"
                     /protein_id="HGU4471963.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINHAHSDTTTSLILPNVKTIPYTLGALESPSTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEMAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDDRTQTNHGIGWRYFTPSWMSGVNMFIDYDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDHDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEEPTLSLADSTLSVDLQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQAKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNDNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGTAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAEINGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSGVVEVSGTDKNETGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTTYTAGGVIKVTVTL
                     KDSYENLVGGQRDAINQAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SDWASALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKVNQSSDSKT
                     VNFVADVRTAQIADLVVIKDGSVADGSTANTLRVRVSDAFGNALDGQTVSVLADNGAT
                     VAPTVITEPDGTVEISVTSQTAGVSAVTASINNSSLSRNVTFVADVRTAKIADLVVMQ
                     DGSVADGTTANTLRARVTDAFGNALAGQTVSVTAGNSATVASTVTTKPDGTVEISVTS
                     QTAGVSTVTASINSSSQSRDVTFVA"
     gene            complement(4236..5420)
                     /gene="nepI"
                     /locus_tag="ACHOQQ_RS18795"
                     /old_locus_tag="pgaptmp_003759"
     CDS             complement(4236..5420)
                     /gene="nepI"
                     /locus_tag="ACHOQQ_RS18795"
                     /old_locus_tag="pgaptmp_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGU4471964.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLCGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            5654..6007
                     /locus_tag="ACHOQQ_RS18800"
                     /old_locus_tag="pgaptmp_003760"
     CDS             5654..6007
                     /locus_tag="ACHOQQ_RS18800"
                     /old_locus_tag="pgaptmp_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4471965.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            5991..6314
                     /locus_tag="ACHOQQ_RS18805"
                     /old_locus_tag="pgaptmp_003761"
     CDS             5991..6314
                     /locus_tag="ACHOQQ_RS18805"
                     /old_locus_tag="pgaptmp_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4471966.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LDVLL"
     gene            complement(6430..6882)
                     /gene="yicN"
                     /locus_tag="ACHOQQ_RS18810"
                     /old_locus_tag="pgaptmp_003762"
     CDS             complement(6430..6882)
                     /gene="yicN"
                     /locus_tag="ACHOQQ_RS18810"
                     /old_locus_tag="pgaptmp_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGU4471967.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(6935..8245)
                     /gene="adeQ"
                     /locus_tag="ACHOQQ_RS18815"
                     /old_locus_tag="pgaptmp_003763"
                     /gene_synonym="yicO"
     CDS             complement(6935..8245)
                     /gene="adeQ"
                     /locus_tag="ACHOQQ_RS18815"
                     /old_locus_tag="pgaptmp_003763"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HGU4471968.1"
                     /translation="MSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMVYIVFVNPQ
                     ILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFVVVGAMGIS
                     WQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALMGLKNTGVI
                     VANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVH
                     FSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLID
                     SNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFL
                     LVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFS
                     ITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
BASE COUNT         2125 a         2256 c         1961 g         1915 t
ORIGIN      
        1 gcgacaaacg tcacatcccg gctctgactg ctgctgttga tgctggcggt gacagtactg
       61 actcccgccg tctggctggt gacactgatc tccaccgtgc cgtccggctt tgtggtcact
      121 gtcgaagcga ccgttgcgct gttgcctgca gtcacgctga ccgtctgccc ggcaagcgca
      181 ttgccgaacg catcggtcac cctcgcccgc agcgtgttcg ccgtcgtgcc atctgccact
      241 gagccgtcct gcatcaccac cagatcagcg atcttcgccg tcctgacatc ggcaacaaac
      301 gtcacgttcc ggctcaggct gctgttgttg atactggcgg tgaccgcact gactcccgcc
      361 gtctggctgg taacagagat ctccaccgtc ccgtccggct cggtgataac cgtcggagcg
      421 accgttgcgc cattgtccgc caacacgctg accgtctgcc cgtccagcgc attaccgaac
      481 gcatccgaca ccctcacccg cagcgtattc gccgtcgaac cgtctgccac tgagccatcc
      541 ttaatgacca ccagatcagc gatctgcgcc gtcctaacat cagcaacaaa gttcaccgtt
      601 ttactatcgc tgctttgatt gaccttagcc tcaacagtgc tggcatccgc tacggtactg
      661 ctcagatcaa cctctgccat accatgcgca tccgtcctga tatctgcgct ctgccctgca
      721 ttggcaaata cagccgcccc cttcgtcacc gtaaaggtga cggtctgctc cggcagcaca
      781 ttgccaaact gatcttccac ccggacattc accgtatgtc gatcgctgcc attcgccagc
      841 acatccgggt tacccgttgt cacttgcatc gcgacaattt gtgcgctggc ggcatcgcca
      901 ctaatactgt aatcatttga agtcagagcg ctcgcccagt cggacatctg tagctgtgct
      961 tttaaaccgg tgccagggag taaggcggta tacgttgcgg tataaattcc tttttgatct
     1021 tcattccagg cgatgctttc cgcttttgta tttggcagct gaatagcctg gttgatagcg
     1081 tctcgttgcc ccccgaccag gttttcatag ctatccttga gtgttaccgt aacttttatc
     1141 acgccgcccg cggtgtaggt cgttttgtcg gtaacgatgg aggaataagc cagcacagct
     1201 ccgctttcgc ggatggcata cgcatcggat tgctgcgcgc tactccatgt cgatagtttc
     1261 agcgtggcgt aatggcgatc gccagcaatc ttcgcggttc gggtggtggt gtaaacgccg
     1321 tcagactctt cgctccagtt acccgtttca tttttatctg taccgctcac ctcgaccaca
     1381 ccgctacccg ccagcagcga agccatccct ttcaccaggt ttttctgctc atccataagg
     1441 gtgacggaca cttttatctt gccacccgca gtgtatgaca catcgtccgt cttgatggtg
     1501 gaggtcagct gcgagatatc gccagtgata aagctcactt ccacagactg actactgcca
     1561 ttgatctcgg ccttgataag actgtttacc gcctggtcac tggcaaggtt aacgctggct
     1621 atcccgtctt tatctgtcgt cacaataccg ccattcggat cggcaaaaac cgccgtacca
     1681 ttcacgaccg tgaaggtaac attttgtccc tgcagtaaat tttgatgcac atccctgacc
     1741 gtcacagcga cggtattgtg atcgcgtctg gcaactgcgt tattcgcggt gatctgcatc
     1801 gcagcgattt tggcggtatc tttatcaccc gcaatactga acgcatccga atgtttggtt
     1861 tgccagccgg gcatcgtcag ctgggccgtt aatttgtcgc caatgagatg ggccgtatag
     1921 cttgctttgt aaattccctc actttcttcc gtccaggcac taacggcatc agccttcgtg
     1981 ttgtctactt taacggtttg tttgagaagt tcttttcgtc cggtaacccc gttgtcgtta
     2041 tcatctctca gggtcacttt aacgactatc ggcttacccg cgacgtaatt atcctggtct
     2101 gtctcaatcg tggagtcagc acgagatgcc gtattcgcca caatggctat cagcgctggc
     2161 gtcttagcga tatcgtcgcc gttaaattgc ggcatcagcg ataacgcgcc agacgtcttc
     2221 ccggcggtaa ctatttgggt ataggttccg ttaccgttgt ctttccattc gcttaaggcg
     2281 aaatcctgca aacctttcgc ctgtgttttt aacgtcatac cggggattgg cttgccgctg
     2341 gaatctcgcg cggtataggt cagcgttgag gttgattttc catcagcaag caggatttgc
     2401 agatcgacag aaagcgttga atcggcgagg cttagtgtgg gttcttccac gaccaccata
     2461 ctttcttcac gccgggagaa atttcctttc gaatcttccg ctgtaaccgc tatcgggtaa
     2521 gtgttgtcgg tttcaggcat tgccgtgaat cggtagggag gaatagtgac ctgaatatcc
     2581 ttgccggaaa cagctacttt tccgcccgct gattgcaggg aggcggcttc aatgtcataa
     2641 cctttcagcg catatttggt ttgtaaggaa gaaaccagcg atttaacctc acccggcttg
     2701 cctttcaacg gatcggtcag cgtcagccgc accagctctt tcttgcgata ttcgaggaca
     2761 atattgttat tacgatcgac caggtcatag cgactgccca caaggctgcg tcgtgctgca
     2821 acttcagccg gatcgagctg tttttgcagt gaatgaccag gttgcaacgt caactccata
     2881 ccaattcggg tgtcgttttc tccctgtttg ccctgccgtt gctcggcact aaaggagatc
     2941 agaggaacag gggtgtaact caaacctgcg gtaatcgcgt gaggatcatt ttgtcgctcg
     3001 tctttaccaa acagcgccac ttcatcgcca taatattgtt catagaccaa cttgccgccc
     3061 agctgcggcc aggccggtaa ccagccttcc gcgcgaagat cccacccgtt cgccggacgt
     3121 gcttcatagt catggtcgag ttccggtgca ctacgccagt tactcaggcg cagataaccg
     3181 ttgccgctca gtttcaggta atcgcgccag tattcaacgc ccatcccggt tcgggtgtga
     3241 tagcgggtca ggtcataatc gataaacata ttgacgccag acatccagct cggggtgaaa
     3301 taacgccagc cgataccgtg gttggtttga gtgcgatcat ccgtacgatg aagtgtgtgc
     3361 tggctgaaga ccaggttatc cggcgtttcg taccagggat ggagaaattc gaatgaagag
     3421 cttttaaggc tgaaatcttc atccacacca agagataccc gggcagttcc ccaacgactg
     3481 agccagtccg ttgcctgcgc agatgcagaa gaagccaccc agccacgcgc catactcgcc
     3541 gcctgttcgc tgtccatatc gcgagctaaa agtgttccgc tttgctgcgc catttccgcg
     3601 acctgcattt gcggatcttt gccgtcgcgc tccatctgca ttgctttcag gttgcgcgct
     3661 tcagggctat tactgttaat gaggggcaca tctatttcat cgccctgacg cacattgtca
     3721 aagccccgcg caaaggtacg aaactggttc aggcgacgta actcatcaac ggtgatacca
     3781 aagcgtgccg cgaccgttga tggcgattcc aatgccccca gggtatatgg aatagtttta
     3841 acgttcggca ggatcaggga cgtggtagta tccgaatgcg catgattaat atgcgtaggg
     3901 gcagcggcca taacagggaa tatagtttgt gttatcaggc aaataccggc ggtcagacga
     3961 cgtaaacgat cccccaatcc acatatggtg ttatcgcccc gacgatcgcc attcccatgc
     4021 gctttccctg ccatttgtca tccctgtcgc tatgcgttca gattaaatga cctttcttta
     4081 acatgaggga aaatgatgat tcatgattag caaatgatga atacaggcgg aataatggaa
     4141 ataacaacaa gataagaaca atacctgatc aagcgttatc agggggaaga acaggatagc
     4201 tttcccgcca gagaaataac cctcctgtaa atacgttact tcattttcac ctttgcggtg
     4261 actaataacg ccgtcagcaa catcaatgtg ccggacaaca tcaacggcga agtcagacca
     4321 atattatcca gcgcataacc gccgattgcc gcgccacagg tattagcaag ctgaataacc
     4381 gccacctgaa tagacccggc tttttctgcc tgatcggcca gtgagcgggt gatccacgtt
     4441 gaccagccga cgggaaccaa tgcaaaagtt agcccccaga taatcgccac gccggtagca
     4501 acgattttat cgcttccaca caacgtcagc accaacgcac tcacagccag tattaacggc
     4561 gcgcctgcta aggccagttt taccgaacgt ttaagaatga acgacgaaag cgacgtaccg
     4621 ataaagctgg cgataccaaa acttaacagc accagcgtta agccatccac accgaatccc
     4681 gccaggttca tatacactgg acgaatatat gtgaagaaag caaactgtcc ggcgaaagac
     4741 atgaagatgg cgatcatccc tgccatcaca cccggacgtt gtaataagcg gaaagtattt
     4801 tgtttctgat gcgagggttc gcctggcagt gaaggcaatg atttgataat ccagaaaata
     4861 cacagcacgc ccatcaccgc cgccgcatta aagacattgc gccaaccgat aagctcgcct
     4921 aaaaaactgc ccaacggcgc ggcaatcacc agcgcaatag aaaccgcgcc gaagatcacc
     4981 gacagcgcct tcggcaccgt acgcggcggt accagacgca tggtcagcga cgccgacatc
     5041 gcccagaacc cgcccagcgc cagccccaga caggcacgac cgattaaaag caaactgaat
     5101 gagttagcaa aggaaaccag caaacaggag agcgtcagca aaacggcaaa taaaataaca
     5161 acgtagcggc ggtcagtggc ctgaattgtc tgggtaataa acaaactggc aaacattgcc
     5221 acaaaggcgg tcacggtcac cgattgcccg gcaacccctt ccgaaatgcc taaatcctgg
     5281 gccattggcg tcaacaaact gacgggcaaa aactcaacga taatcagaca ggcgacacaa
     5341 aacgccaccg agaaaacggc tgaccagttc ggtcgggtga tggcatccgc gccgcggttt
     5401 tcagcaataa attcactcat tgtgttaccc gtggcatgtt tcaggggaaa agccgaccac
     5461 tttaacattg aaatgtgtga cgcatttaac gtttttgcaa cttttacggt gttgtgggaa
     5521 aagttcgagg ctgctcgaag aaacaaaaat catagggcaa gctgatatgc attttcgcga
     5581 ttgggttggg aataagggat aaagtattgc acttggtgat atactttaat aacaggacct
     5641 caacgatatg cgcgtgttta aaaccaaatg gtttgcaagg gaagccaggt ctcatgcaat
     5701 aacagatgaa gagttgtgcc gggccatttt agaaacggag caaggtaaag ctgatgcact
     5761 tggtggtggc gtttttaaaa agcgccttca ccaaaatcgt gagcgtgcaa ttattctggc
     5821 aaaaggggga agcaattggt tttacacgtt tttatatgca aaacaggata tgtcgaacat
     5881 caatagtcag gaactggctg ggtttcgtga acttgcaaag cattatgcct tcctgacgaa
     5941 agcccagcta acagcaatga ttaacactaa agaactgaca gagatctgct atgactgcaa
     6001 aaactaaatt taaaagtcct gcatttgagg ctattcacag cgcagcggct ggattgtcca
     6061 gcgtcgatgc tatttctgcc gaaacaatgc gtacttttga caaagcctgc ctgaccagcg
     6121 tgcaagactt acaacctgtt gagataaaag cactgcggga aaaattaaaa gtgagtcagc
     6181 ccgtcttcgc tcactatctc aataccagcg tttcaactgt acaaaaatgg gaaagtggcg
     6241 ctaaacgccc caatggcatt tcattaaaac tgctcagcat tgtgcaaaaa cacgggctgg
     6301 atgtgctgct gtaaatagaa cgtagataat gcgttcacct actgtcagtt ccgataccgg
     6361 acgcggcgtg aacgccttat ccactcgaca ggtcaggcac ggtctgtagg ctgataagac
     6421 gaagcggttt taatgcagcc agtgaacgcc ctcggcaggc tgaaagaatg cgttatagcg
     6481 catctgaaaa gcattaatat cctgcatttc aggttcagtt tcgcgcagaa aacccagcgc
     6541 cagcctgacc tgagcatcgg taatcggcgc ggcaaggcgg gctttttgta aaatccgatg
     6601 ccattgcagc aggttctgtt cgctaccatc gacaatgaca atttgccaga tgagcaacac
     6661 ctgcgcggcg ttttgcaggt gcgactcatc cggacgatgc aaataacgta aaaattcgtc
     6721 accagctgac tttcccattt gatcgaccat cgactccacg ggtacgacat cgatattcag
     6781 ccgatcgctg agacggcgaa tcgcttttct ggccccggat gtcagcaccc gaaaaccaac
     6841 cacaaacact accgccagcg tggcgagcat tatccagatc atgagttctc ctgcgtaatc
     6901 agaactcatg atatctggaa accctcgcca gttactaatc caccagaata atcttcagtg
     6961 caaacagagc tgcgaccacc accacacaca ggttcagatc gcgccagcgc ccggtgcata
     7021 ctttcatgat gcagtacgac ataaagccga gtgcaatccc ttcggtgatc gagaaagtaa
     7081 agggcatcat caccgtggta ataaatgcgg gcactgattc ggtaaaatca tcccagttaa
     7141 cgcgcgccag gctcgaagtc atcagcacgc caacaaagat taacgctccg gcggttgcgt
     7201 aaggaggaac catcgccacc agcggtgaga agaacataac taacaggaac ataacgccga
     7261 ccacgacagc cgttagcccc gtgcgaccac cgactgccac accagaagta ctttcaatat
     7321 aggcagtaac agacgaggta ccgataaacg cgcccgccac cgaactgacg ctatcaacat
     7381 acagcgcctt attcatattg gggaatttac cgttactatc tattaagcca gctttatcag
     7441 ttacaccaat taatgttcct gatgaatcaa ataggttgat cagcataaag gagaaaatga
     7501 taccggcgag ttcaagtgtt aacgcgccgc tcaaatctac ttcaccaatg acgccgctaa
     7561 tatcaggcgg aatggaatag acgccgctaa aatgaacatc accgaaaaat aatccacagc
     7621 aagacgtcac cacaatagaa accagcaccg cggcatgaaa atgacgtgat gacaacacgg
     7681 ttataataaa aaaccctaaa atacctaaca acacaccgtg agaacttaaa tcgccaatca
     7741 tcaccagggt gtctttattg gcgacaataa cgccagtatt ttttaatccc attaaggcga
     7801 taaataatcc aattccactg gtgataccaa tacgtaaact taatggaatg ttggagatca
     7861 tccagtaccg gatacgaaac agcgtgagca aaaatagccc aattgcaccc cagaatatcg
     7921 cgcccatccc ggtttgccag gagatcccca tagcccccac gaccacgaag gcaaagaagg
     7981 cgttcagccc cattgccgga gccagcgcca cgggtaagtt agcaaatatc cccatcgcaa
     8041 tactgccgat accggcaatc aaacaggtgg taacaaacac cactttcggg tccatttgtg
     8101 ccgcgccgag gatttgcggg ttcacaaaaa cgatgtacac catggttaaa aaagtggtca
     8161 tccccgcaat caattctgtg cggacggtgg tcccatgctg aggtagttta aataatcgcg
     8221 aaagcgtccc tgattcatta ctcacgtaat cggtatt
//
LOCUS       DAZKFU020000208         8227 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZKFU020000208 DAZKFU020000000
VERSION     DAZKFU020000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.210"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..278
                     /locus_tag="ACHOQQ_RS18820"
                     /old_locus_tag="pgaptmp_003764"
     CDS             <1..278
                     /locus_tag="ACHOQQ_RS18820"
                     /old_locus_tag="pgaptmp_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593043.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HGU4471969.1"
                     /translation="SPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAVYEAWQREQNPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            278..5548
                     /gene="traI"
                     /locus_tag="ACHOQQ_RS18825"
                     /old_locus_tag="pgaptmp_003765"
     CDS             278..5548
                     /gene="traI"
                     /locus_tag="ACHOQQ_RS18825"
                     /old_locus_tag="pgaptmp_003765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HGU4471970.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYMDVLARTVGILPPEAGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNIYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GQREVTMTVLSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLESAISHQ
                     KSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEVREQTLIVTHLNEDRRVLNSMIHDSREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGVSKDDGLITLQDAEGNTQLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVFEPRADREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLSGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAEEKPDQPDGKTEQAVRAIAGQEQDRASSSERETALPESVLREPQRVQDAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5568..6314
                     /gene="traX"
                     /locus_tag="ACHOQQ_RS18830"
                     /old_locus_tag="pgaptmp_003766"
     CDS             5568..6314
                     /gene="traX"
                     /locus_tag="ACHOQQ_RS18830"
                     /old_locus_tag="pgaptmp_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HGU4471971.1"
                     /translation="MTTDNTNTTRNDSLAARTGTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNLSRYEHIRQASVNRLWGWAVVAQF
                     GYFLAGYPWYEGNILFAFAVAAQMLKWCEYHSLVWSVSALTLLVLWFPLSSTSYGAVG
                     IILLMLCHRIYRTTHLSVRSMLALCLFPVVALVNLQDGVTAAVSGVLVTYMVVRMVSG
                     IRWPAARFWPADFFPVFYVCHLAVLGVMAL"
     gene            6396..6764
                     /locus_tag="ACHOQQ_RS18835"
                     /old_locus_tag="pgaptmp_003767"
     CDS             6396..6764
                     /locus_tag="ACHOQQ_RS18835"
                     /old_locus_tag="pgaptmp_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HGU4471972.1"
                     /translation="MKKILLVVAITGMFVQGVWAKSDQQIRDEAKNNVITVSQVSGMT
                     EGTGVTLNGQIVKQLAAEEDHFELSDASGSIVIDVDDEVWRPLALKAGDKVQVVGEVD
                     ISDEGNRKATVDVLTLERLR"
     gene            6800..7357
                     /locus_tag="ACHOQQ_RS18840"
                     /old_locus_tag="pgaptmp_003768"
     CDS             6800..7357
                     /locus_tag="ACHOQQ_RS18840"
                     /old_locus_tag="pgaptmp_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGU4471973.1"
                     /translation="MTEQKRPVLTLKRRTDVETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELWAILAE"
     gene            7511..7714
                     /locus_tag="ACHOQQ_RS18845"
                     /old_locus_tag="pgaptmp_003769"
     CDS             7511..7714
                     /locus_tag="ACHOQQ_RS18845"
                     /old_locus_tag="pgaptmp_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471974.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
BASE COUNT         1931 a         1993 c         2702 g         1601 t
ORIGIN      
        1 tgtctcctgc catcaacgat aagaagtcag atgcaggtgt gaatgttccg gcagggggga
       61 tcgagcagga gctgaaaatg aaaccggaag aagagatgga acagcaactg ccacccggga
      121 tcagtgaatc cggtgaagtg gtggatatgg ccgtttatga ggcatggcaa cgggagcaga
      181 atccggatat ccagcagcag atgcagcgcc gtgaagaggt gaatatcaac gtgcaccggg
      241 agcgcgggga ggatgttgag ccgggagatg atttctgatg ttgagtttca gcgttgttaa
      301 atcggcaggc agtgccggaa actattacac cgacaaggat aattactatg tgctgggtag
      361 catgggggaa cgctgggcgg gcaagggggc tgaacagctt gggctgcagg gcagtgttga
      421 taaggatgtc tttacccgtc ttctggaggg gaagctgccg gacggggcgg atttaagccg
      481 tatgcaggat ggcagtaata agcatcgtcc cggctatgac ctgaccttct ccgcccccaa
      541 aagtgtctcc atgatggcca tgttaggtgg cgataagcgc ctgattgatg cacataatca
      601 ggccgtggat tttgctgtcc gtcaggtgga ggcgctggct tccacacggg tgatgacgga
      661 cggacagtca gaaacggtac tgaccggtaa tctggtgatg gcactcttta accacgacac
      721 cagtcgcgat caggacccac agttacacac gcatgcggtg gtggctaatg tcacgcagca
      781 taacggcgaa tggaagacac tgagcagtga taaagtgggg aaaaccggtt tttccgagaa
      841 cgtgctcgcg aaccgtattg cgttcgggaa aatatatcag agtgagttac gtcagcgcgt
      901 tgaggcgctg ggctatgaaa ctgaagtggt gggtaagcac ggtatgtggg agatgccggg
      961 cgtaccggtg gaggcctttt ccggacgctc acaggctatc cgggaggccg tcggggaaga
     1021 cgcctcgctg aaatcccggg atgtggcggc actggatacg cgaaaatcca aacagcacgt
     1081 cgatcctgaa gtcagaatgg ctgaatggat gcagacgctg aaggaaaccg ggttcgacat
     1141 ccgggcgtat cgtgacgcgg cagaccaacg ggcggagacc cgcacgcaga cacccggacc
     1201 cgcttcacag gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag
     1261 tgaacgaaaa gtgcagttca cgtacatgga cgtgctggcc aggacggtcg gcatactgcc
     1321 gccggaagcc ggtgtgattg agcgggcgcg tgccggtatc gatgaggcca tcagccgtga
     1381 gcagcttatc cccctcgacc gtgagaaggg gctgttcacg tccgggattc atgtgctcga
     1441 tgagctgtcc gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgactgtaca
     1501 tccggagaaa agtgttcccc ggacggccgg ttacagcgat gcggtcagtg ttctggcaca
     1561 ggatcgcccg tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg
     1621 ggtggctgaa ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttattgccgc
     1681 tgaccgtcgc tcacagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac
     1741 cggacgtcgt cagctgctgg aaggcatggc cttcacgccg ggcagtaccg ttatcgtcga
     1801 ccagggcgaa aaactctccc tgaaagagac gttaaccctg ctggacggtg ccgcacgtca
     1861 taacgtacag gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcgctgat
     1921 ggccatgaag gatgccgggg tgaacatata tcgctggcag gggggagagc agcgaccggc
     1981 aaccatcatc agtgaaccgg accgtaatgt ccgctatgcc cggctggccg gagattttgc
     2041 ggccagcgtg aaagccggag aagagagcgt ggcacaggtc agcggagtac gggaacaggc
     2101 catactgaca caggccattc gcagtgaact gaaaacgcag ggcgtactcg ggcagaggga
     2161 ggtgaccatg acggtgctgt caccggtctg gctggacagc cggagccgtt atctgcggga
     2221 tatgtaccgc ccgggaatgg tgatggagca gtggaacccg gagacacgca gtcatgaccg
     2281 ctatgttatc gaccgggtga cagcgcagag tcacagcctg accctgcggg atgcgcaggg
     2341 cgaaacgcag gtggtgcgta tatcctccct ggacagcagc tggtcgctgt tccggccgga
     2401 aaaaatgccg gtggcagacg gcgagcgact gagggtgaca gggaaaattc ccggactccg
     2461 cgtctccggc ggtgaccgcc tgcaggtggc atccgtcagt gaagatgcga tgacggttgt
     2521 tgtgccgggg cgggctgaac cggccaccct gcctgtggct gattcaccgt tcacggcact
     2581 gaagctggag aatggctggg tggaaacgcc cgggcattcc gtcagtgaca gtgcgaaggt
     2641 ttttgcctcc gtcacacaga tggcaatgga caacgccacc ctgaacggtc tggcccgcag
     2701 cggtcgtgat gtccggctgt attcctcact ggatgaaacc cgtactgcgg aaaaactcgc
     2761 ccgccatccg tcctttacgg tggtttctga gcagataaag gcgcgtgccg gtgagacatt
     2821 gctggaaagt gccatcagtc atcagaagtc cgcacttcac acgccggcac agcaggccat
     2881 tcatctggcc cttcctgtgc tggaaagtaa aaacctggcc ttcagcatgg tggacctgct
     2941 gacagaggcg aagtcgtttg ctgcagaagg aaccagtttt actgagctgg gaggggaaat
     3001 caatgcgcag ataaaacgcg gtgatttact gtatgtggat gtggcaaaag gctatggcac
     3061 aggcctgctg gtttcccgtg cgtcgtatga ggcagaaaag agcattcttc gccatattct
     3121 cgaaggtaag gaggcggtca cgccgctgat ggagagggta cccggcgaac tcatggagac
     3181 actgacgtcg gggcagcgtg ccgccacccg catgatactg gaaacgtccg accgtttcac
     3241 ggtggtgcag ggttatgccg gcgtgggtaa gaccacacag ttccgggcgg tgatgtcagc
     3301 cgtgaagatg ctgccggaga gtgagcgtcc ccgtgtcgtg gggctggggc ccacgcaccg
     3361 tgcggtcggg gagatgcgca gcgccggcgt ggatgcgcag acactggcgt cttttctgca
     3421 tgacacgcag ctgcagcagc gcagcggaga aacgccggat ttcagcaaca cgctgttcct
     3481 gctcgatgag agctctatgg tgggcaatac cgacatggca cgggcatacg ccctgattgc
     3541 ggccggtggt ggtcgtgccg tggccagcgg tgacacggac cagctgcagg ccatcgcgcc
     3601 cggtcagcct ttccgtctcc agcagacgcg cagtgctgcc gatgtggcca tcatgaaaga
     3661 gattgtgcgt cagacgccgg aactgcggga ggcggtatac agcctgatta accgggatgt
     3721 ggaaagggca ctgtccgggc ttgagagtgt gaaaccgtct caggtgccac gtctggaggg
     3781 tgcatgggca ccggagcact ccgtgacgga gttcagtcac agccaggaag cgaaactggc
     3841 agaagcgcag cagaaggcga tgctgaaagg cgaggctttc ccggatgttc ccatgacact
     3901 gtatgaagcc attgtccgcg actataccgg caggacaccg gaagtacggg agcagacgct
     3961 gattgtcacg cacctgaatg aggaccggcg cgtactgaac agcatgattc atgattcacg
     4021 ggaaaaggcc ggtgagctgg ggaaagagca ggtcatggtg cctgtcctga atacagcgaa
     4081 tatacgcgac ggggaactgc gtcgtctctc cacctgggag aataacccgg atgcccttgc
     4141 cctggtggat aatgtgtatc accggattgc cggtgtcagt aaggatgacg ggctgataac
     4201 cctgcaggat gcggaaggta acacgcagct gatttcgccc cgggaggcag tggctgaagg
     4261 cgtcacactg tacaccccgg acaccatccg ggtgggaacc ggtgaccgga tgcgcttcac
     4321 gaagagtgac cgggagcgcg gttatgtggc caacagcgtc tggactgtga cagcggtttc
     4381 cggtgacagt gtcacgctgt cggacggaca gcagacccgg gtgattcgcc ccggccagga
     4441 gcaggcagag caacatattg acctggccta tgccatcacc gctcacggtg cgcagggggc
     4501 gagtgaaacc tttgccatcg cgcttgaagg cacggaaggt aaccggaaac agatggccgg
     4561 ctttgagtca gcctatgtgg ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga
     4621 taaccgtcag ggctggacgg atgccatcag caaagccgta cagaaaggaa cggcccacga
     4681 tgtgtttgag ccaagggctg accgggaggt catgaatgca gagcggctgt tcagtacggc
     4741 gcgggagctg cgggatgtgg cagctggtcg tgctgttctc cgtcaggcag ggctgtccgg
     4801 gggagacagt cctgcacggt ttattgcacc ggggcgcaaa tatccgcagc cgtatgtggc
     4861 cctgccggct tttgaccgta acggcaagtc cgccggtatc tggctgaacc cactgaccac
     4921 ggatgacgga aacgggctgc ggggattcag tggtgaaggc cgggtgaagg gcagtgaaga
     4981 tgcacagttt gtggccctgc agggcagccg taacggcgag agcctgctgg cggataatat
     5041 gcaggatggt gtccggattg cccgtgataa ccccgacagc ggtgtggtgg tgagaatcgc
     5101 cggtgaaggt cgtccgtgga atcccggtgc catcaccggt ggtcgcgtgt ggggggatat
     5161 cccggacaac agcgtccagc cgggggccgg aaacggtgag ccggtcacgg cagaggtgct
     5221 ggcacagcgg caggctgaag aggccatccg tcgtgaaacg gaacgccgtg cggatgagat
     5281 tgtcagaaaa atggcagagg aaaaacctga ccagccggat ggtaaaacag agcaggctgt
     5341 cagggctatt gccgggcagg agcaggacag ggcttcgtca tcagaacggg aaactgcgct
     5401 gccggagagt gtgctgcgtg agcctcaacg ggtgcaggac gctgtccggg aggttgcccg
     5461 ggaaaatctg ctgcaggagc gacttcagca gatggagcgg gatatggtcc gtgacctgca
     5521 gaaagagaaa accctgggcg gagactgata caggaagaca aaggctgatg acaacggata
     5581 acacgaacac gacacgtaac gattcactgg ctgcccggac cggtacctgg ctgcagtcgt
     5641 tgctggtctg gtcacccgga cagcgggata tcatcaaaac ggtggcactg gtgttgatgg
     5701 tgctggacca tgcaaaccgc attctgcatc tggagcagac gtggatgttt ctggccgggc
     5761 ggggcgcgtt tccactgttt gcgctggtct ggggactgaa tctgtcacgg tatgagcata
     5821 tccgtcaggc gtccgtaaac cgcctgtggg gatgggctgt tgtggcgcag ttcgggtact
     5881 ttctggcggg atatccgtgg tatgagggca atattctgtt tgcctttgcg gtggcagcac
     5941 agatgctgaa atggtgtgaa tatcactcgc tggtatggtc agtcagtgca cttacacttc
     6001 tggttctctg gttcccgtta tcttccacca gttacggtgc tgtcgggatc atattactga
     6061 tgctctgtca caggatatac aggacgacgc atctttctgt acggagcatg ctggcgctct
     6121 gtctttttcc ggtcgttgct ctcgttaacc tgcaggacgg cgtgactgcc gctgtcagtg
     6181 gtgtgttagt gacatacatg gtcgtcagga tggtatccgg tatccggtgg cctgctgccc
     6241 gtttctggcc ggcagacttt ttcccggtat tttatgtctg tcatctcgct gtgctgggag
     6301 tgatggccct gtagcaggaa gcgcagaaag cggatgaact gtgaaagtct ttttgtttaa
     6361 atcagaacgc attcaggaaa aaacaggagc agatgatgaa aaaaatatta ctggtggtag
     6421 ccattacggg aatgttcgtt cagggggtat gggcaaaatc agatcagcaa atccgggatg
     6481 aggctaaaaa taatgtgatc actgtttctc aggtgagtgg catgacggag gggaccggtg
     6541 tgacgctgaa tggtcagata gtgaagcaac tggcagccga agaggatcat tttgaactca
     6601 gtgatgcatc aggaagtatt gtcattgatg tcgatgatga agtatggcgc ccgctggccc
     6661 tgaaagccgg agataaagtt caggttgtgg gagaagtgga tatttctgat gagggaaacc
     6721 ggaaagccac ggtggatgta ctgactcttg agcgtcttcg ctgatatgaa caacggatac
     6781 cggtgagggc aggtgagtta tgacagagca gaaacggcca gtactgacac tgaaacgaag
     6841 aaccgacgtg gaaacaccga cccggagccg gaaaaccatc atcaatgtca ccacgccacc
     6901 aaaatggaag gtgaaaaagc agaaactggc ggagaaggct gcccgggaag cagagctggc
     6961 ggcgaagaaa gcacaggcca gacaggcgct gtccatttat ctgacgctgc cctcgctgga
     7021 tgaggcggtg aacactctga aaccctggtg gccgggattg tttgacggta acacgccccg
     7081 gcttcttgcc tgcggtatcc gggaggtttt actggatgag gtgtctcagc gaaatatccc
     7141 gctctctcat aaaaaactca gccgggcgct gaaagccatc acccgttcgg aaagttatct
     7201 gtgcgcaatg aaagccggcg cctgccgcta tgatacggaa ggctatgtga cagaacatat
     7261 cacacaggaa gaagaacagt atgcgcaggc gcgcctggaa aaggtgcgca gacagaacag
     7321 gatcaaggat gagctgtggg cgatcctggc ggaataataa aaacgccctc tgtatgacag
     7381 agggcaacag cgaaaaaatc gggaaaacag acatccagag ttttgatgga gaaaaacacc
     7441 caaccgttca gggatgtttc agaatgtttc tgacataatc cgggagccgg gtcaagaggg
     7501 gggataaaaa atggctgtat ccagtggatt atctgagcac agcggtattt atcgctatca
     7561 gcgcgtgggg cgtcatgccg tccgtcagga gattcgtggt tttttcaggg aagccgctga
     7621 ggcctggcag aatgcggccc ggctggcacc ctgtgaagca tggcgggatt ttgccctgaa
     7681 gagagcatct gcatgccggt gtaagtgccg ataaaaataa cccccgttgc ggagcgccgg
     7741 gggatcaggc ttatatggca gatcgttatt attatagtgg tgtaagaagg agttgccctg
     7801 tctttcaaac ggacatcaca gagttaacat atttcacatc ccgggtaagc cagcagggat
     7861 aaaaaatgtg atgaatttca agaagcagga ggtgacagtg gcatgcataa cagcagggtt
     7921 atccggtgaa tctgacagac agtcgggctg tattcgcggc aggataaaga aaaaccggga
     7981 gatgactctc ccggtccgtc ttatcgctaa tcccctgagc agggcatgta tgacttatct
     8041 atgaccgtta tattcagcgc cacatccggt agcctgcgat ataacacagt tatcctatca
     8101 gcaagagcag gaatgtttaa tactcagggg tgaaaaatat aatctgtatc acaaagtagt
     8161 atgtagcagg gggaagccga tagggtacga acggggaacg ggaatacatg accttgcgaa
     8221 caggaaa
//
LOCUS       DAZKFU020000209         8189 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZKFU020000209 DAZKFU020000000
VERSION     DAZKFU020000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.211"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..500
                     /locus_tag="ACHOQQ_RS18850"
                     /old_locus_tag="pgaptmp_003770"
     CDS             <1..500
                     /locus_tag="ACHOQQ_RS18850"
                     /old_locus_tag="pgaptmp_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HGU4471975.1"
                     /translation="FLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQR
                     KMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALV
                     VISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMP
                     SLPFL"
     gene            complement(556..3411)
                     /gene="valS"
                     /locus_tag="ACHOQQ_RS18855"
                     /old_locus_tag="pgaptmp_003771"
     CDS             complement(556..3411)
                     /gene="valS"
                     /locus_tag="ACHOQQ_RS18855"
                     /old_locus_tag="pgaptmp_003771"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGU4471976.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEYN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(3411..3854)
                     /gene="holC"
                     /locus_tag="ACHOQQ_RS18860"
                     /old_locus_tag="pgaptmp_003772"
     CDS             complement(3411..3854)
                     /gene="holC"
                     /locus_tag="ACHOQQ_RS18860"
                     /old_locus_tag="pgaptmp_003772"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGU4471977.1"
                     /translation="MKNATFYLLDNDITVDGLNAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(4208..5719)
                     /gene="pepA"
                     /locus_tag="ACHOQQ_RS18865"
                     /old_locus_tag="pgaptmp_003773"
     CDS             complement(4208..5719)
                     /gene="pepA"
                     /locus_tag="ACHOQQ_RS18865"
                     /old_locus_tag="pgaptmp_003773"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGU4471978.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5873..5923
                     /gene="ytgA"
                     /locus_tag="ACHOQQ_RS18870"
                     /old_locus_tag="pgaptmp_003774"
     CDS             5873..5923
                     /gene="ytgA"
                     /locus_tag="ACHOQQ_RS18870"
                     /old_locus_tag="pgaptmp_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGU4471979.1"
                     /translation="MSIIILATISTTQENK"
     gene            5986..7086
                     /gene="lptF"
                     /locus_tag="ACHOQQ_RS18875"
                     /old_locus_tag="pgaptmp_003775"
     CDS             5986..7086
                     /gene="lptF"
                     /locus_tag="ACHOQQ_RS18875"
                     /old_locus_tag="pgaptmp_003775"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4471980.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7086..8168
                     /gene="lptG"
                     /locus_tag="ACHOQQ_RS18880"
                     /old_locus_tag="pgaptmp_003776"
     CDS             7086..8168
                     /gene="lptG"
                     /locus_tag="ACHOQQ_RS18880"
                     /old_locus_tag="pgaptmp_003776"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4471981.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
BASE COUNT         1770 a         2184 c         2176 g         2059 t
ORIGIN      
        1 catttttagc tttattacct tttctcgctg ttgctggtta tattatcttc gatcaaatat
       61 taaatgcgta tgatagttct gtgtatgcaa atgatgatat tgagaattta cagcaattta
      121 tggaaatgca acgtaaaatg ataatcgcgc agttaatcta ttattttggg attgctgtta
      181 gcaccagtta tttaacggtg tcgttgcgaa atcattttat gagcaacctg tcactgaatg
      241 atgggcgtat tcgttttcgc tcaactttaa cataccacgg tatgctttat cgcatgtgtg
      301 cgttggtggt gatatccggg attacgggcg gtctggctta tccactgctg aaaatatgga
      361 tgattgactg gcaggcaaaa aatacgtatt tgctgggcga tttggatgac cttcctttaa
      421 tcaataaaga agaacaacca gataaaggct tcttagccag tatttcacgg ggaattatgc
      481 cttctttacc atttctgtaa gagataaaaa aggccggagc atgctccggc cttcgttttc
      541 atcactgtgt tttgattaca gcgcggcgat aacagcctgc tgttcaatca gtttcgcttt
      601 cgcttctgca tagccttcca gcttctcacg ctctttcgcg atgaccgctt ccggtgcgcg
      661 ggcgacaaag ccttcattcg ccagcttgtt ctcgatacgg ctgatttcgc cttcgatctt
      721 cgccacttct ttcgccagac gcgccagctc atcttctttg ttgatgaggc cagccatcgg
      781 gatcagcagc tctgcgccgt cgatgatctt cgtaacggaa accggacctt tgtcatcggc
      841 aggcagcacg gtgatgcttt ccagacgcgc cagcgtttgc aggaagccac ggttttcatt
      901 tacgcgacgt tctgcatccg cgctgcaacc acgcagcagc agctccagcg gtttgcccgg
      961 cgcgatattc atttctgcac ggatgttacg tacggcaacg atcgcctgtt tcagccactc
     1021 ggtgtcggcc agcgccgctt catcaacctg agacgcatca tactgcggga acggctgcag
     1081 catgatggtg tcggcagtga taccgcaaag tactttcaca cgctgccaga tggtttcggt
     1141 gatgaacgga atgatcggat gcgcgaggcg cagcagacct tccagtacag tcaccagcgt
     1201 atggcgagta ccgcgcagtt cggcttcggt gccaccgttc attaccggct tggtcagctc
     1261 cagataccag tcgcagaact ggttccaggt gaactcatac agaatgcctg cagcgatatc
     1321 gaagcggaag ctgtccagcg cttcgcggta cgctttgatg gtctggttgt actctgccag
     1381 aatccagcgg tccgccagcg acagcgtcat ttcaccaccg ttgaagccgc aatcctgacc
     1441 ttctgtgttc atcagcacaa agcggctggc gttccacagc ttgttacaga agttacggta
     1501 accttccagg cgcttcatat cccagttgat gtcacgaccg gtagaagcca gcgccgccag
     1561 ggtgaagcgc agggcatcgg tgccgtgcgg ctcaatgccg ttcgggaact gtttctcggt
     1621 gcgcttacgg attttgtccg ccagctgcgg ctgcatcata ttgccggtac gtttttccag
     1681 cagttctggc agcgaaatac cgtcaaccat atccagcgga tcaataacgt tacccttgga
     1741 tttagacatc ttctggcctt cgtcatcacg aatcaggccg gtcatgtaaa cggtgtggaa
     1801 cggcacctgc ggtttgccat tttcatcttt gatgaagtgc atggtcatca tgatcatgcg
     1861 ggcaatccag aagaagatga tgtcgaagcc ggataccatc acgctggttg ggtggaactg
     1921 acgcagggcg tcggtgtttt ccggccagcc aagggtagag aaggtccaca gtgcagaaga
     1981 gaaccaggtg tcgagaacgt cttcgtcctg acgcagggca acgttggcac cgaggttatt
     2041 ttccttgcgc acttcttctt cgttgcggcc aacataaaca ttacccgctt cgtcatacca
     2101 tgccgggata cggtgacccc accacagctg acgagagata caccagtcct gaatatcgcg
     2161 catccaggag aagtacatgt tttcgtactg cttcggtacg aactgaatgt cgccgttctc
     2221 aaccgcttca accgccggtt tcgccagtac atcggcacgc acgtaccact ggtcggtcag
     2281 cattggttcg ataactacgc cgccacggtc gccgtaagga acggtcaggt cgtgcggttt
     2341 aatttcttcc agcaggccga gtgcgtcaac cgcagcaacg accgctttac gtgcagcaaa
     2401 acgctcgagt ttctggaact ctgccgggat ttcgctggaa taaacgtcag attcgttacc
     2461 tttggtatcg aacacctggg cgctttcacg gatatcgccg tcaaaggtca ggatgttgat
     2521 catcggcagc gcgtgtcgtt tacccacttc atagtcgtta aagtcgtgcg ccggagtgat
     2581 tttcacgcag ccggtgcctt tttccatgtc ggcgtgttcg tcgccaacga tcggaatacg
     2641 acggttaacc agcggtagaa tgacatattt gccaatcaga tctttgtaac gcggatcttc
     2701 cgggttaacg gctacgccag tatcgcccag cagggtttcc ggacgggtag tcgcgaccac
     2761 cagataatct ttaccgtctg cggttttcgc accgtcagcc agcggatagc ggatgtgcca
     2821 catcgaacct ttcgattcgc ggttttccac ttccaggtca gagatagcgg tgcgcagttt
     2881 cgggtcccag tttaccaggc gtttgccacg gtaaatcagg tcttctttat acagacgaac
     2941 gaaaacttct ttcaccgcat tggacaggcc ttcgtccatc gtgaagcgtt cacgctccca
     3001 gtcgacggag ttgccgagac ggcgcatctg acgggtaatc gtgccgccgg attccgcttt
     3061 ccattcccag attttgtcga tgaaagcttc gcggccgtag tcgtgacggg ttttaccttc
     3121 ttctgcggca atcttgcgct caacgaccat ctgggtagcg atcccggcgt ggtcagtacc
     3181 gacctgccac agggtgtttt tgccctgcat gcgctgatag cggatcatgg tatccatgat
     3241 ggtttgctgg aaggcgtgac ccatatgcaa actgccggtg acgttcggcg gcgggatcat
     3301 gatgcagaaa ctttcctggc tttcatcgcc attaggctta aagtagccct gcttttccca
     3361 gtgctcgtaa agcggctgtt cgatatcttg tgggttatat gtcttttcca ttatttccag
     3421 gttgccgtat tcaggttgaa accagccacg cggtaggctt tatagcgttc gcgcgccagt
     3481 tgtttcagag aatcttcata aggaacgaag tctaccactt ctgtgaaagc ggtggcaaaa
     3541 tctgcaaagc ttgttcgcag actaatcaat atatcgcgcg ggctgctgct gcgcttttgc
     3601 ggccaggcga tctccaccgg cgcaccgccg cgcggtcctt ctcccgctaa attatgcgga
     3661 acaaagcttt ctgccggacg cgcccacagg gcttcatcca gtcggtaagc ctgcttttca
     3721 tcttcacagg cgatgagtac gcgcttacca ctgcgccaac gttctgcggc aatttcacac
     3781 accagttgct caacggcgtt taagccatcg acggtgatgt cattgtccag aaggtagaac
     3841 gtcgcgtttt tcatatatgg ggcttcttgt ggtggattta aatacgcatc aggtatacac
     3901 cgcagcgcct gatgcgacgc tgttgtgtct tatcaggcct acgagttcag tgctgtgtgt
     3961 aggtcggata aggcgctcac gccgcatccg acaataacag cattgcctga tgcgacgctg
     4021 ccgcgtctta tcaggcctac gagttcggtg ctgtgtaggt cggataaggc gttcatgccg
     4081 catccgacaa taacagcatt gcctgatgcg acgcttgcgc gtcttatcag gcctacgagt
     4141 tcagtgctgt gtaggtcgga taaggcgttc acgccgcatc cggcaataac agccttgcct
     4201 gacgcaatta ctcttcgccg ttaaacccag cgcggtttaa caggaactgt gccagcaacg
     4261 ctaccggacg accggtggcg ccttttgctt taccagaacg ccaggcggta ccggcgatat
     4321 ccaggtgcgc ccagttgtac ttacgggtaa agcgcgacag gaagcaacct gcggtaatcg
     4381 ccccaccagg acgaccgcca atgttcgcca tatcggcaaa attggactcc agttgttcct
     4441 gatactcgtc acccagcggt aagcgccatg cgcggtcacc ggattgttca gacgcggcaa
     4501 tcagttcatg ggccagcgga ttatggttcg ccatcagacc agtaatatga tgacccagcg
     4561 cgatcacgca ggcaccggtc agcgtcgcca cgtcaatcac cgcttccggc tcaaaacgct
     4621 caacgtaagt taacacgtcg cacagtacca ggcggccttc agcgtcagta ttcagcactt
     4681 caacggtttg accggacatg gtggttaaca catcgcccgg acgataggct cgtccgccag
     4741 gcatgttttc gcagcctgcc aacacgccga taacgttaat cggcagttgt agctccgcga
     4801 ccatccgcat cacgccgtaa accgctgccg caccgcacat atcgtacttc atctcatcca
     4861 tgccttctga aggcttgatc gagataccgc cggagtcgaa ggttaaacct ttacccacca
     4921 gcacgattgg gcgtgcatct tccgacgcgt tgcctttgta ctcaatcacc gacatcagcg
     4981 attcgttttg cgaaccctga ccgaccgcca gataggaatg catccccagc tctttcatct
     5041 gctgttcgcc gataacgcgg gtgataacat tcttgctgta gctgtcagcc agctggcgcg
     5101 cttgtgaagc gaggtaagcg gcgttacaga tattcggcgg catattgcca agatcctttg
     5161 ctgctttaat cccggcggca atcgccagac cgtgctggat cgcgcgctca ccgctggtca
     5221 gttcacggcg ggtcggcacg ttgaacacca ttttacgcag cggacgacgc ggttcgctct
     5281 tgttcgtttt cagctgatcg aaactgtaga gcgtctcttt tgccgtctcg acagcctgac
     5341 gcactttcca gtagttgtta cggcctttaa cgtgcagttc agtcagaaag cagaccgctt
     5401 ccattgagcc agtatcattc agcgtattaa tggttttctg aataacctgc ttgtactgac
     5461 gctcatccag ctcacgttct ttgccgcaac caataaggag aattcgctcg gaaagtacat
     5521 tcggaacatg gtgcagcaac aatgtctgcc ccggttttcc ttccagttcg ccccgacgta
     5581 gcagggcgct gatgtaccca tcgctgattt tatcgagctg ttctgcaatc ggagaaaggc
     5641 gacgtggttc gaagacgccc acgacgatgc aggcactccg ctgtttctcc gggctaccgc
     5701 tttttacact aaactccatg cactacgctc ctgaatctta aagacaacgg cggtggctac
     5761 agatagaatt gcaagctttc gtaactcatg tccgctgttg cgatgacttc gtgttaatct
     5821 taacgttatt acggcattgg cacgtcagaa caaaatctga gaggtgaatc cgttgagtat
     5881 aattatctta gcgacgattt cgacgactca agagaataaa tgacgtttaa gccatgaaac
     5941 aagctaaaat cctgcaaaag acgagttttt acgggcgtat ttaaagtgat aatcataaga
     6001 tatctggtgc gggagacgct caaaagccag ctggcgatac tcttcatctt gcttttgatc
     6061 ttcttctgtc aaaagttagt gaggatcctc ggcgcagcgg ttgacggcga tattccggcg
     6121 aatctggtgc tctcccttct cgggttgggc gtgccggaaa tggcgcagct tatcctgcca
     6181 ttaagcctgt tcctcgggct gctgatgacg ctgggcaaac tgtataccga aagtgaaatt
     6241 acggtaatgc atgcctgcgg cctgagcaaa gcggttctgg tgaaagcggc aatgatcctt
     6301 gcggtattca cggcaatcgt cgcggcggtt aacgtgatgt gggcgggacc gtggtcatcg
     6361 cgtcatcagg atgaagtgtt agcagaagcg aaagcgaacc ctggcatggc ggcgctggcg
     6421 caagggcaat tccagcaagc gactaatggc agctcggtgc tgttcatcga aagcgttgac
     6481 ggcagcgatt tcaaagatgt gttcctcgca caaattcgac caaaaggtaa tgcacgtcct
     6541 tctgtggtgg tggccgattc cggacattta acccagctgc gcgacggctc ccaggtcgtc
     6601 actctcaacc agggaacgcg cttcgaaggc actgcactgt tacgtgattt ccgcattacg
     6661 gatttccagg attatcaggc gatcattggt caccaggcgg tggcgctcga cccgaacgat
     6721 accgaccaga tggacatgcg cacattgtgg aacactgaca ccgatcgtgc tcgcgcagaa
     6781 ctgaactggc gtatcacgtt ggtattcacc gtgtttatga tggcacttat ggtcgtaccg
     6841 ctgagcgtgg ttaacccacg tcagggacgc gtactgtcga tgctgccagc catgctgctg
     6901 tatctacttt tcttcctgat ccagacctcc ctgaaatcga acggcggtaa aggtaagctg
     6961 gacccgacgc tgtggatgtg gaccgttaac ctgatttatc tggctttggc gattgttctc
     7021 aacctttggg acaccgtgcc ggtccgccgc ctgcgcgcca gtttttcgcg taaaggagcg
     7081 gtgtgatgca accttttggc gtacttgacc gctatatcgg taaaactatt ttcaccacca
     7141 tcatgatgac gctgttcatg ctggtgtcgc tgtcgggcat tatcaagttt gtcgatcagc
     7201 tgaaaaaagc cgggcagggg agttacgacg cgttaggcgc aggaatgtat accttgctga
     7261 gcgtgccgaa agatgtgcag atcttcttcc cgatggcggc tctgcttggg gcgttgcttg
     7321 gtcttgggat gctggcgcag cgcagcgaac tggtggtgat gcaggcttct ggttttaccc
     7381 gtatgcaggt ggcgctgtcg gtgatgaaaa ccgccattcc gctggtctta ctgacgatgg
     7441 cgattggcga atgggtcgcg ccgcagggcg agcagatggc gcgtaactac cgtgcgcagg
     7501 cgatgtacgg cggctcgttg ctctctaccc agcaagggtt atgggcgaaa gatggcaaca
     7561 acttcgtcta cattgagcgg gttaaaggtg acgaagagtt aggtggcatc agcatttatg
     7621 cctttaacga gaatcgtcgt ctgcaatccg tacgctatgc cgctactgcg aagtttgacc
     7681 cggaacataa agtctggcgt ctgtcgcagg ttgatgaatc tgatctgacc aatccgaaac
     7741 agattaccgg ttcgcagacg gtgagcggca cctggaaaac caacctcaca ccggacaaac
     7801 tgggcgtggt ggcgctggac ccggatgcac tctccatcag tggtttgcac aactacgtga
     7861 agtatctgaa gtcgagcggt caggatgccg gacgttatca gctcaacatg tggagcaaaa
     7921 tcttccagcc gctatccgtg gcggtgatga tgctgatggc gctgtcgttc atctttggcc
     7981 cactgcgtag cgtaccgatg ggcgtgcgtg tggtcaccgg tatcagcttc ggttttgtct
     8041 tctacgtact ggaccagatc ttcggtccgc tgacgttggt ttacggcatc ccgccgatca
     8101 ttggcgcact gttgccaagc gccagcttct tcttaatcag cctgtggctg ttaatgagaa
     8161 aatcgtaatc taatgaacgc ataaaggga
//
LOCUS       DAZKFU020000210         8184 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZKFU020000210 DAZKFU020000000
VERSION     DAZKFU020000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.212"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(310..1167)
                     /gene="murI"
                     /locus_tag="ACHOQQ_RS18885"
                     /old_locus_tag="pgaptmp_003777"
     CDS             complement(310..1167)
                     /gene="murI"
                     /locus_tag="ACHOQQ_RS18885"
                     /old_locus_tag="pgaptmp_003777"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGU4471982.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1112..2995)
                     /gene="btuB"
                     /locus_tag="ACHOQQ_RS18890"
                     /old_locus_tag="pgaptmp_003778"
     CDS             complement(1112..2995)
                     /gene="btuB"
                     /locus_tag="ACHOQQ_RS18890"
                     /old_locus_tag="pgaptmp_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU4471983.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     regulatory      complement(3060..3250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3364..4464
                     /gene="trmA"
                     /locus_tag="ACHOQQ_RS18895"
                     /old_locus_tag="pgaptmp_003779"
     CDS             3364..4464
                     /gene="trmA"
                     /locus_tag="ACHOQQ_RS18895"
                     /old_locus_tag="pgaptmp_003779"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGU4471984.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMITGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4504..4863)
                     /gene="yijD"
                     /locus_tag="ACHOQQ_RS18900"
                     /old_locus_tag="pgaptmp_003780"
     CDS             complement(4504..4863)
                     /gene="yijD"
                     /locus_tag="ACHOQQ_RS18900"
                     /old_locus_tag="pgaptmp_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGU4471985.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4863..5513)
                     /gene="fabR"
                     /locus_tag="ACHOQQ_RS18905"
                     /old_locus_tag="pgaptmp_003781"
     CDS             complement(4863..5513)
                     /gene="fabR"
                     /locus_tag="ACHOQQ_RS18905"
                     /old_locus_tag="pgaptmp_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGU4471986.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5617..6933)
                     /locus_tag="ACHOQQ_RS18910"
                     /old_locus_tag="pgaptmp_003782"
     CDS             complement(5617..6933)
                     /locus_tag="ACHOQQ_RS18910"
                     /old_locus_tag="pgaptmp_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HGU4471987.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYATAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6976..8142)
                     /locus_tag="ACHOQQ_RS18915"
                     /old_locus_tag="pgaptmp_003783"
     CDS             complement(6976..8142)
                     /locus_tag="ACHOQQ_RS18915"
                     /old_locus_tag="pgaptmp_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HGU4471988.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKLWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2181 a         2200 c         1882 g         1921 t
ORIGIN      
        1 attcattttt cgtcttgcga cgttaagaat ccgtatcttc gagtgcccac acagattgtc
       61 tgataaattg ttaaagagca gtgccgcttc gctttttctc agcggcgcgg ggtgtgcata
      121 atacactttc ccgctacaga gtcaagcatt tatttttgct ttctctgccg gaattctcag
      181 gagaatcccg ctgacccggc ggcgtgtttg ccgttgttcc gtgtcagtgg tggcgcatta
      241 tagggagtta ttccggcctg acaagaggaa atttaaaata attttccgac cgcgcaacat
      301 tcaaccaaat cagcctaaaa ctgccagttt ttcgagcgtt tcgaagccgt aacgctgtaa
      361 aacgggcaat aattgttcag cttctggcgt cattgccata caaaaggcaa tattcgcatc
      421 ggcagatttt gcatccgggg cttcatgttc taacaaccaa gccgttcggc gagcaatcgc
      481 tgcgccagaa tccaccagcc gggttccctc tggcagcact tgtaacagtt cttcttgtag
      541 tagagggaaa tgggtgcaac ccaatacaac ggtatctggc ggctctttca ttcttaacca
      601 cgggcgtagg atacgtttta gtgcatccag agaaacatct tcgccatgca gcttcgcttc
      661 agccaactca accatctctg ccgagcccag catttctatc tggcattcat tagcgaaacg
      721 cgcgatcagc tcatgagtat aagaacgttt aactgttccg cgggttgcca gtaatccgac
      781 aatgccattt gccgtcagac gtgcagcagg tttaatcgcc ggaacgacac caacaaccgg
      841 gaagtcgaac ttttcgcgta aggcaggaag tgaaacggta ctggcggtgt tgcaagcgac
      901 cacagccagc gcaaggggat aacgttcttg caccgcggtg acaattgcca ccactcgctc
      961 aacaataaac gcttcgcttt tttcgccata cgggaaagcg acgttatcga aagcataaat
     1021 gtaatggaga tccggtaaga gatgccggat ctcgtcatag accgacaacc caccgacgcc
     1081 ggagtcaaac accagcacgg tgggacgtgg ttcagaaggt gtagctgcca gacaaggtgt
     1141 attcccgtcc tgcagtttgg tagccataga ctgtctcata atctttgtcg aacaggttgg
     1201 ctattttacc acgaactgtc aggtgagagg tgaccggata tgcaaccgca agatcccaca
     1261 agctcacacc gcctaattta actgtatgtg atgagccaga ggtctcgtca taagcgttgt
     1321 cgtatcgttg acctatatat tggtaagaaa cgtcccattc gaaaccataa gcttgtccag
     1381 tcaactcgta cttaacctgc tgttttgccc gacggtacag cactttatct gtttcatcat
     1441 cgcgcgggtc aacatattgt aacgtcaagc gatgttcaac aggaccggtt gtaatatttc
     1501 ccgtccattc cagcccttta atcgtggctg atttcacatt aatgtactgg ttgacattat
     1561 ttatgctttt ataatcaatc aggttctgaa tttcataacg atatcctgag agccgccaat
     1621 ccagagtccc agttaccccc tcaattccaa tctcccactg tttagattct tctggcttca
     1681 aatttgggtt ggctgcaata ccaggaccat aaattgaagc aaagcgagca gccccgtatt
     1741 gctgtccaag agaaggcgaa agaaaactgg taccgtaaga aagtgttgcc tggtagccat
     1801 caataaactc ccagccagca gctgtctgcc aggtaccatg ccagccaaac tgttcatcat
     1861 gatcttctcg gccagaagct tccaaagtca cattggaaat ctgttgctgt ccagtcaggt
     1921 ataacccggt ggtatcgcgt tcgtaatcat ctgattttgt aatgctgctg gacgtcagtt
     1981 tttcctgttt ccaatcgatc ccgccactaa ttgcaccatg ccctacctca atgctattac
     2041 cccactgaat ataacgctgt tccatgtcat ccagggtggc atctcccgca taacgaccaa
     2101 gattatttgt gtagttataa ttcttgagtc gttgatagtt agcaattaac tgagacgaat
     2161 agatgccgga gttatagcgt aggccagtat cccaggactg gttgtaattc tgagcttctt
     2221 cgttgccagt agcagaacca tacatcgagc cctggtcgta atcattatta gctgtgtagc
     2281 tgtaaccacg gaagaacccc gagaagttat cgttaaactt atgctgcagc cccccccaga
     2341 aaagtttgtt gcgatatcca tcgcgatcgc tatcaccact gtatggtgag ccaggctgaa
     2401 tgttaaaacc tttagtcgtc tggtaagcac ctgctgctgt taccatcgta tcgccaaaac
     2461 gtttatttat taccccatca taggcttgat aaccatttgt acccattcct gcgttgatct
     2521 gagctttctc atcattactc attgtaataa tgttcaccac acctccgatg gcaccagaac
     2581 cataaacggc agaacgcggc ccacgaatat attcaacacg ctgtacaagt gataccggga
     2641 actggctaat atcaattgca ttgtcgatac cggcacgagc catcggcaca ccgtcaatca
     2701 acactaaaac atggcgcgat tcagtgccac gaacataaag agaagaactc gtccccattc
     2761 caccagtttg agagatattt acaccgggga gatgactcat aacatcgttg aggtctttcg
     2821 actgccagcg ttgaatatcc tcacgggtaa caatatcagt aggtgcaagg acagtattta
     2881 ctggctgctg aaaacggtta gcagtaacga cgagagtatc cgggctggta tcctgtgccc
     2941 aagcggaaaa ggctgtgaca gaacacgccg tcagcagcga agctttttta atcattgtaa
     3001 agcatccaca atagaagaag gatgccgcag gtttcatcaa tattacgcga tgatgagaac
     3061 cagatgcgac gttagccggc aggtcttcgg gcttggaggg gtatctaaga tactaagata
     3121 tgatgacttc ccaccgaatg gcagtgtccg cataacgcaa tcatcgcacc tttccttacc
     3181 gctgcgcgtc agctccagat tcgcactgga ttccctatta actcacagga ccggcaagtg
     3241 gatgctacag gttgtaacaa gttactgtcc agacgtagct cacaaatagg aattcatcaa
     3301 gatctggaca tctgatgagc aatccctaca atcgccgcgt actttaattt ttcaggatac
     3361 atcatgaccc ccgaacacct tccaacagaa cagtatgaag cgcagttagc cgaaaaagtg
     3421 gtacgtttgc aaagtatgat ggcaccgttt tctgacctgg ttccggaagt gtttcgctcg
     3481 ccggtcagtc attaccggat gcgtgcggag ttccgcatct ggcatgatgg cgatgacctg
     3541 tatcacatca ttttcgatca acaaaccaaa agccgtatcc gcgtggatag cttccccgcc
     3601 gccagtgaac tcatcaacca gttgatgacg gcgatgatta cgggtgtgcg taataatccc
     3661 gttctgcgcc acaagttgtt ccagattgat tacctcacca cgctgagcaa tcaggcggtc
     3721 gtttccctgc tataccataa aaagctggat gatgagtggc gtcaggaagc ggaagccctg
     3781 cgcgatgcac tgcgagcaca gaatctgaat gtgcatctga ttggtcgggc aacgaaaacc
     3841 aaaatcgcgc tggatcaaga ttacatcgac gaacgtctgc cggtcgcagg gaaagagatg
     3901 atctaccgtc aggtagaaaa cagctttacc cagccgaacg cggcgatgaa tattcagatg
     3961 ctggaatggg cgctggacgt aaccaaaggc tcaaaaggcg atttactgga gctgtactgc
     4021 ggcaacggta acttctcatt agccctggcg cgcaattttg atcgggtatt agccaccgaa
     4081 atcgccaagc cgtcagtcgc tgccgcgcaa tacaatattg ccgctaacca tattgataac
     4141 gtacaaatta ttcgtatggc ggcagaagaa tttactcagg cgatgaatgg cgtgcgcgag
     4201 tttaaccgcc tgcaagggat cgacttaaag agttatcagt gcgaaaccat ttttgtcgac
     4261 ccaccgcgca gcggtctgga cagtgaaacc gagaaaatgg tgcaggcgta tccacgtatt
     4321 ctgtatatct cctgcaatcc ggaaacgtta tgcaagaatc tggaaacatt aagccagacg
     4381 cacaaggtcg aacgtctggc tctgtttgat cagttcccct acacgcacca tatggagtgc
     4441 ggcgtattac tgaccgcgaa gtaaaaccca tgccggatgc gccagcatcc ggcatgatac
     4501 cgattactca gcaacttcct gcttacggtt acgcatcttc gcgccaatcc agaacaccat
     4561 aatgacggag agtactgccg ggaagaagtt agagccgata tccggatatt ccgcacgaac
     4621 taccgtgctg tacagcagta cgccgagaat aaaacaggca gctgccagac ccggcaagcc
     4681 taccggcatg gtgcgattaa gataacgttg atgcaggcag taaaccgtca gcaccaggga
     4741 aataatcggg aatacagaaa atggcacaat ggagctaaac agcgctgcga aagtaccatt
     4801 aatcgataag ccagcaacca acgccagcag cagcgtacct ctatcttgat ttgcttgttt
     4861 cattactcgt ccttcacatt tcccggaata attgcggttt tctcttgttc acggcgatac
     4921 cagtaataag cccctttcga aatcattcgc agttgcagta ccagtcgctc ttctaattgc
     4981 cgacgttgtt cgacgccgac gtccaacgcc tcggcacccg cactgaagac aattgtcacc
     5041 attgcttcgg cttgcgcttc agtaaacgca cgcggcatat ggttttcgag ttccagatag
     5101 tccgcaagtt ccgcaatgaa gtgctgaatt tcacgcgcaa cggcggcacg aaacgcagcg
     5161 gaggtgccgg agcgttcccg caataataac cggaaggcgt taggattatt accgatgaac
     5221 tccataaatg tggagaccga ggtacggatc acactcccgc ctttggcgat acgctgacgc
     5281 gcctggcgca tgagttggcg tagcattaaa ccgctctcat caaccatggt cagacccagt
     5341 tcgtctacgt cgcggaaatg ccgataaaaa gaggtgggag caatgcccgc ttcacgcgcc
     5401 acttcacgca aactcaggct ggcgaagctg cgttcagcac ttaattggct aaatgcggct
     5461 tccaccagcg aacggcgggt tttttctttt tgttgcgctc ttacgcccat cacgatgtct
     5521 gaatccttgc caaaagtact gctggcacta taccagagaa tgaacatact ggattgctcc
     5581 agtattgtga atgaacggta acgcaataaa acatatttac tgcaattctt tggccggttc
     5641 ttttacgtac aacaacgcca acatcgacag caacgtgcct ccacccaaat accacgccgg
     5701 agccgtcaaa ctatccagca acacgatgga ttgcgtagcg aaatattgag caaaaccgcc
     5761 aaagattgcc acaccaatgc tataaaccag cgcaaaaccc aatgcccgaa tccgtttcgg
     5821 caacagttca gagatcaaca acatcactgg cacaccgcca agcgtggtaa agctgaccat
     5881 cacgaaaacc accgtcagca acatgccagg gcttgggtaa ttcaccaaca gccagaatga
     5941 cggccagcta cagatcagca ccatcacacg ggagattaaa atcagctttt tacgcccgac
     6001 cgaatcgcac aacatcccca ccagcagcgc acccacaaac gtaataacgc ccgccagtaa
     6061 catcgctgca tgagaatagt tttggttcat tcctaaatac ttcgccgccc atgtaccgta
     6121 ataaaagagg gagatatagg ttgcgactgt actaccaata gcgagcaaga taccgttagc
     6181 gatagtcgcc ttatgttgca ggagcagaga aaacgcgctt tcgctggtag cagctttttt
     6241 attgcgcaca ggctcaggta cgtcattttc cagactcaaa cgcaaccaac agccgacagg
     6301 cgctaataac acgccaataa aaaacggcac acgccagccc cattcagcca tgacggtttc
     6361 tgaaccggtc gcaaagggca gccatgcact taaacctaaa gcgacaacac ctccaaaagt
     6421 tgttgccagg ccttgagtcg ccagtgacca actgctgtaa aaaccgcgac gatttgccgg
     6481 agctgactcc accagtagcg acattgaagc cccaacttca ccgcctgctg caacaccctg
     6541 aatcaaacga gcaagtacca gagttgccgt tccccaatag cccgccgtcg catacgtcgg
     6601 cgcaatgcca atcatcaacg tgccgaggct catcagggca atagtgaaca ccatcgcggg
     6661 tttgcgtccg aaacgatcgg cccaggcccc gacgataatt ccaccaagcg ggcgcataaa
     6721 aaaactcacg ccaaacgttg caaatgccag taacagcgcg acattgtgat cagcgctggg
     6781 tgggaagaaa agtgtactga tatagaccac aaaaaagttg taaacgccaa aatcaaaaaa
     6841 ttcgagcgca ttgccaaacg ccgaggcaaa aatcacccgc ctgccgctgg gctttttctt
     6901 cacactcacg gtttcattta cgatcgccgt cattccatgt tccttgccaa aaagtgttgt
     6961 tgtgtgttgt tttatttatt gcagccaggc ttctaccagt gcgccccagt agctgcttgc
     7021 cgggaccaga cttgcatcgt tgaagtcgta acccgggtta tgcaccatgc aggaaccttc
     7081 cccgacatcg ccattaccga ataacaggaa accgcctttg ggttgggctt ccagcatgaa
     7141 agagaaatct tcactgccca tcagaggctt gatgccaaac tcggcccgat cttcaccaaa
     7201 cgttttacac gccacctgcc aggcgaagcg cgccatctcc tcgtcattta ctaatactgg
     7261 tgtaccgtta acatgcgtaa cctcagccgt agcgccaaag ctggcagcct gggcttgtgc
     7321 cagagcagga atacgggtca gaagaagttg tcgggtatcg cggctaagag agcggacgct
     7381 gattttcatc tcagcactgt caggaatgac gttagccgcc tcacccgcaa caatgctgcc
     7441 gacggtaatt accgccgctt ccagcggatc gacattgcgt gacacaatgc tttgtaatgc
     7501 ggtggtgata tgtgcggcaa ccagcaccgg atcgatggct ttgtgcggga tcgcaccgtg
     7561 tccgccacaa ccacgaaccg taatatggaa ctgatccatt gacgccatca gtgcccctgg
     7621 ctggaaataa aacttaccca ccggcagacc gggcatgtta tgcatgccga aaatgacatc
     7681 gcaggggaaa tgatcaaaaa gcccctcttt aaccatgatc tcaccgccat taatcatctc
     7741 ttccgcaggc tggaagataa gacgtaacgt gccgttaaaa cggcgggttt cggcaaaata
     7801 acgcgcggcc cccagcagta tggtggtatg cccgtcatgc ccgcaggcgt gcattaaacc
     7861 aggatgttta ctggcccaga gcttgccact attttcataa attggcagcg catccatatc
     7921 cgcccgcagg ccaatacttt tctctccgtc gcccactttt aacgtggcca ctatccccgt
     7981 cgtcgccagt ccttgctcta cttcatagcc ccactgacgc agcttttccg caaccaaagc
     8041 actggtttta aattcttgta atcccagctc cgggttttcg tggatctgat ggcggatctc
     8101 acgcagttct tcctcaaacc ctcgaatata gtgttcgatc atcggctatt cctttattca
     8161 cttataaaca ggctaattaa cgga
//
LOCUS       DAZKFU020000211         8182 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZKFU020000211 DAZKFU020000000
VERSION     DAZKFU020000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.213"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            243..1013
                     /locus_tag="ACHOQQ_RS18920"
                     /old_locus_tag="pgaptmp_003784"
     CDS             243..1013
                     /locus_tag="ACHOQQ_RS18920"
                     /old_locus_tag="pgaptmp_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGU4471989.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1004..1477
                     /locus_tag="ACHOQQ_RS18925"
                     /old_locus_tag="pgaptmp_003785"
     CDS             1004..1477
                     /locus_tag="ACHOQQ_RS18925"
                     /old_locus_tag="pgaptmp_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGU4471990.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1584..2123
                     /gene="dnaT"
                     /locus_tag="ACHOQQ_RS18930"
                     /old_locus_tag="pgaptmp_003786"
     CDS             1584..2123
                     /gene="dnaT"
                     /locus_tag="ACHOQQ_RS18930"
                     /old_locus_tag="pgaptmp_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGU4471991.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKTEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2126..2863
                     /gene="dnaC"
                     /locus_tag="ACHOQQ_RS18935"
                     /old_locus_tag="pgaptmp_003787"
     CDS             2126..2863
                     /gene="dnaC"
                     /locus_tag="ACHOQQ_RS18935"
                     /old_locus_tag="pgaptmp_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGU4471992.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2912..3406
                     /gene="yjjA"
                     /locus_tag="ACHOQQ_RS18940"
                     /old_locus_tag="pgaptmp_003788"
     CDS             2912..3406
                     /gene="yjjA"
                     /locus_tag="ACHOQQ_RS18940"
                     /old_locus_tag="pgaptmp_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGU4471993.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3660..5951
                     /gene="opgB"
                     /locus_tag="ACHOQQ_RS18945"
                     /old_locus_tag="pgaptmp_003789"
     CDS             3660..5951
                     /gene="opgB"
                     /locus_tag="ACHOQQ_RS18945"
                     /old_locus_tag="pgaptmp_003789"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGU4471994.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQTVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6089..7111)
                     /gene="lgoD"
                     /locus_tag="ACHOQQ_RS18950"
                     /old_locus_tag="pgaptmp_003790"
     CDS             complement(6089..7111)
                     /gene="lgoD"
                     /locus_tag="ACHOQQ_RS18950"
                     /old_locus_tag="pgaptmp_003790"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HGU4471995.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATLEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7250..8164
                     /locus_tag="ACHOQQ_RS18955"
                     /old_locus_tag="pgaptmp_003791"
     CDS             7250..8164
                     /locus_tag="ACHOQQ_RS18955"
                     /old_locus_tag="pgaptmp_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGU4471996.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVMARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
BASE COUNT         2064 a         2127 c         1995 g         1996 t
ORIGIN      
        1 cagtcaccta ttatcctgat tcgtaaaggc taaataatga attcattata gtgataatat
       61 tcatgtgata atttgtttgc ggcgagagat aattcgcttt ttatcaccga gcttctcccg
      121 ataaccagga ttaatcagcg caattttgat cttaacctac cgcaaaacaa ccgtgcttca
      181 tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag
      241 ttatgcaaac tgagcaacag cgagccgtaa cacggctttg tatccagtgt ggattatttc
      301 ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg
      361 cgctgggaat ggacagcgta gaaagttcta tctcttcgaa cgccatagtg ctgacaacta
      421 ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc attaatatgc
      481 atgtggtgac tgaagtccag cacattgtga ttcttgcgga gcatcatctg ctggattaca
      541 aaggcgtaga gaaacgattt agccaaattc agccattacg ttacccaaga tggctggtag
      601 ccttaatggt tggcctttct tgcgcctgtt tctgtaaact caataacggt ggctgggatg
      661 gtgccgtcat caccttcttt gccagtacgg ccgcgatgta tatccgccag ctgctggcac
      721 aacgtcatct tcatccacaa atcaactttt gcctcaccgc tttcgccgcc accaccattt
      781 ccggattgct tttgcaactc cccactttca gcaatacccc caccattgcg atggccgcca
      841 gcgttctgct gctggttccg ggctttccgt tgattaatgc cgtcgccgat atgtttaaag
      901 gccacatcaa taccggactg gcacgctggg cgatcgccag tctgctgaca ctggctacct
      961 gcgtcggcgt agtgatggca ctgacgattt gggggctacg cggatgggtg tgatcgaatt
     1021 tctgttagcg ttggcgcagg atatgatcct cgccgccatt cctgcggtcg gctttgcgat
     1081 ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg ctgcttggct cgataggtca
     1141 tggttcccga atgatcttga tgaccagcgg gttgaatatt gagtggtcaa cctttatggc
     1201 ttcgatgctg gtcggtacca ttggtattca atggtcgcgc tggtatctgg cgcatccgaa
     1261 agtgtttacc gtggcggccg ttatccctat gttcccgggc atatcggctt ataccgcaat
     1321 gatttcggcg gtaaaaatca gccagttagg ttacagcgaa ccgttgatga ttaccctgtt
     1381 aaccaacttt cttacagctt catcgattgt tggtgcgtta tccatcggtc tttccattcc
     1441 tggattatgg ttgtaccgca agcgccctcg cgtataaaat tgcctcgctt aagcacacgg
     1501 atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc ttctcgttca
     1561 tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac gtcgttggta
     1621 ttgacgccct ggtacacgat caccaaaccg ttctggcaaa aactgaaggc ggtgtggttg
     1681 ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc ctggctgaac
     1741 tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga tgtcgatctg gacgatcaac
     1801 tctatcagga accgcaagcc gctcccgttg ctgtacccat ggggaaattc gccatgtatc
     1861 cggactggca acccgatgcc gactttatcc gcctggcggc gctatggggc gtggcgctaa
     1921 gagagccggt gaccaccgaa gaactggcct cattcattgc ctactggcag gcggaaggta
     1981 aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa atcggtcgtg
     2041 ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagtgaacct gacagccaaa
     2101 ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc aacgcctgca
     2161 aaaaatgatg cctgcccata tcaaacctgc attcaaaacg ggtgaagagc tactggcctg
     2221 gcagaaagaa caaggggcga tccgctccgc cgctctcgaa cgtgaaaatc gggcgatgaa
     2281 aatgcagcgc acctttaacc gctccggtat tcgtccactg catcagaact gctcctttga
     2341 gaactatcgc gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc gccagtatgt
     2401 cgaagagttt gacggcaaca tcgccagctt catcttttct ggtaagccag gaaccggcaa
     2461 aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat ccgtattgat
     2521 catcaccgtg gccgatatta tgtcagcgat gaaagatacc ttcaggaata gcggtaccag
     2581 cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg atgagatcgg
     2641 cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg atcgccgctc
     2701 ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga tgaccaagtt
     2761 gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg tgatcttcaa
     2821 ctgggatagc taccgtagcc gggtaacagg taaagagtat taatccccgt ttctggcggt
     2881 atactgcgac acaattccac cttgagtaat gatgatgaaa actgttaaac atcttctgtg
     2941 ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt cctggaaaga
     3001 tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac agcacgacac aggaaggcgg
     3061 ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga aaccaggcct taagcgcaga
     3121 taacatgaac aatgccgcag gcattctgca atactgcgcg aagcaaaagc tggcgtcggt
     3181 aaccgatgcc gaaaacatca agaaccaggt gctggaaaag ctgggcctga acagtgaaga
     3241 gcaaaaagaa gacaccaact atctggacgg cattcagggt ttgctgaaaa ccaaagatgg
     3301 tcagcaactc aatctggata acatcggaac aactccgctg gcagaaaagg tgaaaaccaa
     3361 agcctgcgat ctggtgttaa aacaggggct gaacttcatt tcctgaaccg cgaaacgccg
     3421 cgttttagct gttacccggc tgacgcggct ctctcctgcc cttttccctg ctcctggacg
     3481 gttttacccc tgttacacgg gaagtgaccg taatcaaaaa agtctgttta taaactaatt
     3541 cctaacaaca tcgcattttt ttgacactag aattgcagca atatggcgac gccattacat
     3601 tgtgcttctc aaaaagctta aacgttggct aaacggcccg gtttagtgag gatcgcccgt
     3661 tgtcagaact actctctttc gccctttttc tcgcctctgt gctgatttac gcatggaaag
     3721 cgggacgtaa cacctggtgg tttgcagcca cgttaacggt gctggggcta tttgtcgttt
     3781 taaatatcac cctgtttgcc agcgactatt ttactggcga tggtattaac gacgcggttc
     3841 tctatacctt aaccaacagc ctgaccggtg ctggcgtcag caaatacatc ctgccgggta
     3901 tcggcattgt gctggggctg acggcggtgt tcggtgcgct gggctggatc ctgcgccgtc
     3961 gtcgtcatca tccgcaccat tttggttaca gcctgctggc actcttactg gcgctgggtt
     4021 cggtggacgc cagcccggca tttcgtcaga taacggaact ggtgaaatcc cagtcacgcg
     4081 atggcgaccc ggactttgcg gcttattata aagagccgtc gaaaactatc cctgacccga
     4141 aactcaacct ggtttatatc tacggcgaaa gtctcgagcg gacctatttt gataacgaag
     4201 ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg gatttcagcc
     4261 acacgcagca gctgccagga acggattaca cgattgcggg catggtggct tctcagtgcg
     4321 gcataccgct gtttgccccc tttgaaggca acgcctccgc ttctgtttcc agcttcttcc
     4381 cgcagaacat ctgtctgggc gatatcctga aaaactcggg ttatcagaac tatttcgtgc
     4441 agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct gaagtcgcat ggcttcgacc
     4501 acttatacgg ctcagaagag ctgaaaagcg tggtagccga cccgcactat cgcaacgact
     4561 ggggattcta cgacgatacc gttctcgatg aagcgtggaa aaagtttgaa gagctttccc
     4621 gctcaggtca gcgattctca ctgtttaccc tgacagtcga tacccatcac ccggatggtt
     4681 ttatctcgcg cacctgtaac cgcaaaaaat atgattttga cggtaagccg aatcagtcat
     4741 tcagcgcggt aagttgcagc caggagaata tcgcgacgtt tatcaacaaa atcaaagcgt
     4801 caccgtggtt taaagatact gtcatcgtcg tctcttctga ccatttagcg atgaacaaca
     4861 cggcgtggaa atacctcaat aagcaggatc gcaataacct gttttttgtc attcgtggcg
     4921 acaagccgca gcaagagacg ctggcagtga agcgtaacac gatggataac ggcgcgacgg
     4981 tactggacat tctcggtggc gataactatc tcggacttgg tcgtagcagt ttatccgggc
     5041 agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac attggcgtgg aagccagata
     5101 tcatccgcct gtggaaattc cctaaagaga tgaaagagtt caccatcgac cagcagaaaa
     5161 acatgattgc cttctcgggt agccatttcc gtttgccgct gctgttgcgg gtttcagaca
     5221 aacgcgtgga accgctgccg gaaagcgaat actcagcacc gctgcgtttc cagctggccg
     5281 atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg ctacaagatg gcacaactct
     5341 gggctccgga actggcactt tccaccgact ggtgtgtctc gcaggggcag cttggcggtc
     5401 agcaaactgt tcagcatgtc gacaaaacaa catggaagag caagacggca tttaaagata
     5461 cggtcatcga catggcgcgt tacaaaggca atgtcgatac gctgaagatt gttgataacg
     5521 atattcgcta caaagccgac agtttcatct ttaacgtcgc cggtgcgcca gaagaggtga
     5581 aacagtttag cgggatttcc cgtccggagt cgtggggccg ctggtccaac gcgcagctgg
     5641 gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa gaaatttgac ctggtgatta
     5701 ccgccaaagc atacggcaat aacgccagcc gtcctattcc ggtacgcgta ggcaatgaag
     5761 aacaaactct tgtgctgggc aatgaagtga ccaccaccac actgcatttc gataacccaa
     5821 ccgatgccga cacactggta attgtgccgc cggaacctgt ctcaaccaac gaagggaata
     5881 tcctcggaca ctcgccgcgt aagctcggga tcggcatggt ggaaattaaa gtggtagaac
     5941 gtgaagggta attcctgact gatgtgtagg tcgcataagg tgcgacctac acaccttctc
     6001 cggcttacaa cacattatta agaaacaaaa aaaacagcgc ccaacacttc cccggacgct
     6061 gcatggcaac tttaataatc tcgccttatc agaaagtaat tacgccttta attaactcac
     6121 gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt ggcgaacgga tagcgatggg
     6181 ttaacatcat gtcagcagtg atttttcctt ccgccatcag tcgaccgact ttagcaaaat
     6241 cttccagcgt ggcgttgcgg ctacccatca tcgtcgtttc ttttttatgg aattcaggat
     6301 cggagaactg caactctcct ttaaacaggc cgacaaatac caccgtgccg ccgtgacgaa
     6361 tcagattcac ggtgttattc atcgcatgtt gattgcctgt cgcgtcgatc actttctgcg
     6421 ccagcgaacc accaaactgc gcccgcagct gcgcgtcaaa atcttcggct gacgggtcca
     6481 gcacgggcaa ctccagacgc gttgccacat gttcacggcg cgccggactg gtatccgcca
     6541 ccaccacctg tgcgccatcg gctttagcga ttgccgccgc gcccagaccg attggccccg
     6601 ccccaaccac cagcacctgc tcgccgggag caattgctgc gcgacgcacc gcatgagcgc
     6661 taatagcgaa aggttcaatc aatgcggccg cctgcgggtc aataccgtct gcgggcaaaa
     6721 tgttcgccac cggcacgctc aaatactcac taaaaccgcc atcctgatgc acgccaatga
     6781 ctgaaatttt ttcacagcaa ttggtacgcc cgcttttaca cgccgggcat tgctgacagg
     6841 caacataagg gatcacagca acttgctgac catttttaag atcagcaata tttttaccca
     6901 gcccaacaat ctccccacat atttcatggc ctaaaacccg tggataacta aaaaatggtt
     6961 gatttccacc ccaggcatga atatcggtac cgcaaatccc gacagactta atttttatta
     7021 atgcttcatt gtcacccgga ataggtatct cgcgttgttt ccagactaat tctttcggct
     7081 gctggcaaat taaaacattc atcgtagaca tgattcggct ctccattttt tgttgcctta
     7141 gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat ctgttttgtg aaaacacacg
     7201 cataaaaatg ttttaaatcg ggttttaatg gactcacaag gaggaaaata tgagtcgttc
     7261 acaaaattta cgccacaatg tgattaacca ggtcattgat gatatggcgc ggggtcatat
     7321 cccttcccca cttccatcgc aaagtgcgct ggcggaaatg tacaacatca gccgcactac
     7381 agtgcgtcat attctcagcc acttacgcga atgcggcgtc ttaacgcagg ttggaaacga
     7441 ctatgttatg gcgcgtaaac ctgaccatga tgacggtttc gcctgtacta ccgcttcaat
     7501 gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg atgataaacc agcgtcagtt
     7561 acgccctggg gaaacttttt ctgaactgca actggctcgg gccgcgggtg tcagcccggt
     7621 ggttgtaaga gaatatcttt taaaattcgg ccgttataat cttattcaaa gcgaaaagcg
     7681 cggccagtgg agcatgaagc aattcgatca atcctacgcc gagcagctgt ttgagctacg
     7741 cgaaatgctc gaaacacatt cacttcagca ctttctcaac ctgcctgatc atgatccccg
     7801 ctggctgcag gcgaaaacca tgctcgaacg tcatcggcta ctgcgtgaca acatcggcaa
     7861 tagctttcgc atgttctcgc aacttgacag agattttcac tccttactgc tttcagctgc
     7921 cgacaatatc ttttttgatc aatcgcttga gatcatctca gtcatctttc attttcacta
     7981 tcaatgggac gaaagcgatc ttaaacagcg caatatcatt gccgtcgatg agcatatgac
     8041 cattctcagc gctctgattt gccgcagtga ccttgatgcc acccttgcgc tccgtaacca
     8101 tttgaattca gctaaacaat caatgattcg atccattaat gagaacacgc gttatgctca
     8161 ttaagtacgg attaaaaaca gc
//
LOCUS       DAZKFU020000212         8141 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZKFU020000212 DAZKFU020000000
VERSION     DAZKFU020000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.214"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..300
                     /locus_tag="ACHOQQ_RS18960"
                     /old_locus_tag="pgaptmp_003792"
     CDS             <1..300
                     /locus_tag="ACHOQQ_RS18960"
                     /old_locus_tag="pgaptmp_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4471997.1"
                     /translation="NLRTFNTNELTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTK
                     DTNWNSTYIYKSRHEILPVNLTPETLFGSKSQDKHALFPIFTASWRANRIKNKGI"
     gene            523..1020
                     /gene="ubiC"
                     /locus_tag="ACHOQQ_RS18965"
                     /old_locus_tag="pgaptmp_003793"
     CDS             523..1020
                     /gene="ubiC"
                     /locus_tag="ACHOQQ_RS18965"
                     /old_locus_tag="pgaptmp_003793"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGU4471998.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1033..1905
                     /gene="ubiA"
                     /locus_tag="ACHOQQ_RS18970"
                     /old_locus_tag="pgaptmp_003794"
     CDS             1033..1905
                     /gene="ubiA"
                     /locus_tag="ACHOQQ_RS18970"
                     /old_locus_tag="pgaptmp_003794"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGU4471999.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2060..4483)
                     /gene="plsB"
                     /locus_tag="ACHOQQ_RS18975"
                     /old_locus_tag="pgaptmp_003795"
     CDS             complement(2060..4483)
                     /gene="plsB"
                     /locus_tag="ACHOQQ_RS18975"
                     /old_locus_tag="pgaptmp_003795"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGU4472000.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPTHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4654..5022
                     /gene="dgkA"
                     /locus_tag="ACHOQQ_RS18980"
                     /old_locus_tag="pgaptmp_003796"
     CDS             4654..5022
                     /gene="dgkA"
                     /locus_tag="ACHOQQ_RS18980"
                     /old_locus_tag="pgaptmp_003796"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU4472001.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5132..5740
                     /gene="lexA"
                     /locus_tag="ACHOQQ_RS18985"
                     /old_locus_tag="pgaptmp_003797"
     CDS             5132..5740
                     /gene="lexA"
                     /locus_tag="ACHOQQ_RS18985"
                     /old_locus_tag="pgaptmp_003797"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGU4472002.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5813..7138
                     /gene="dinF"
                     /locus_tag="ACHOQQ_RS18990"
                     /old_locus_tag="pgaptmp_003798"
     CDS             5813..7138
                     /gene="dinF"
                     /locus_tag="ACHOQQ_RS18990"
                     /old_locus_tag="pgaptmp_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGU4472003.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLFGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFSAT"
     gene            7254..7463
                     /gene="yjbJ"
                     /locus_tag="ACHOQQ_RS18995"
                     /old_locus_tag="pgaptmp_003799"
     CDS             7254..7463
                     /gene="yjbJ"
                     /locus_tag="ACHOQQ_RS18995"
                     /old_locus_tag="pgaptmp_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGU4472004.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7505..8020)
                     /gene="zur"
                     /locus_tag="ACHOQQ_RS19000"
                     /old_locus_tag="pgaptmp_003800"
     CDS             complement(7505..8020)
                     /gene="zur"
                     /locus_tag="ACHOQQ_RS19000"
                     /old_locus_tag="pgaptmp_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGU4472005.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1838 a         1996 c         2227 g         2080 t
ORIGIN      
        1 aatttaagaa cctttaacac gaatgaatta acacgaaacg ataatatctt tatcttgttc
       61 tcctctgtcg atgatgggcc agttatggtg gtaagctccc agcgcttaca tgatatgttg
      121 aatcctacaa aagataccaa ttggaattcc acgtatatct acaaatccag acatgagata
      181 ttgcctgtta atcttactcc ggaaacactt ttcggctcca aatctcagga taaacatgca
      241 cttttcccca tttttactgc gagttggcga gctaatcgta taaagaataa aggtatttaa
      301 gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat ggttgccctc
      361 gccagcacgg gcatcggcaa agcgtaaggt tcaacagcgt tttaccactt catgcgattg
      421 ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt aaacactttt
      481 ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca ccccgcgtta
      541 acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggagcc gcaactgctc
      601 gactggctgt tgctggaaga ttccatgaca aaacgttttg aacagcaggg aaaaacggtg
      661 agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaattcccga agaactgccg
      721 ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc cgatggtgaa
      781 ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc ggagctggcg
      841 ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc gacattaacc
      901 cgggacttta ttgagatagg ccgtgatgcc ggactgtggg ggcgacgttc ccgcctgcga
      961 ttaagcggta aaccgctgtt gctaacagaa ctgtttttac cggcgtcacc gttgtactaa
     1021 gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt tcatcgctta
     1081 atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt atgggcgttg
     1141 tgggtggcga caccgggcgt tccccagctc tggatcctgg cggtgtttgt cgcgggtgtc
     1201 tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgcaa gtttgatggt
     1261 catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga gaaagaggcg
     1321 cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtgctgac gctgaatacg
     1381 atgaccattc tgttgtcgat tgccgcgcta gcgctggcgt gggtgtaccc gtttatgaag
     1441 cggtataccc atctaccgca agtggtgctg ggcgcggcgt ttggctggtc gattccaatg
     1501 gcttttgctg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt cctcgccaat
     1561 attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgtga tgatgatgtg
     1621 aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt gattattggt
     1681 attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa tggcttaggc
     1741 tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca acaaaaactg
     1801 attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa ctatgttggt
     1861 ctggtactgt ttttagggct ggcaatgagt tactggcatt tctgatgatg taaaaaagcc
     1921 ggatgatcat ccggctttct tctgggttgc ctgatgcgct gcgcttctca ggcctacaca
     1981 acagatcgca atttattgaa tttgcagatt atggaaggcc ggataaggcg tttgcgccgc
     2041 atccggcaat tctctctgat tacccttcgc cctgcgttgc actctcaatc gtcaaacgca
     2101 cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct gccggttcgg
     2161 catcgccgct atcgctgata tacccttcat cacgtagcgt cagcaccaga gaactgaaca
     2221 ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga cgttgcgcga
     2281 cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttggcg ctcaacagcc
     2341 agaaggtgat agcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc agctgtagcg
     2401 tgcgagaatg cgtcgggttg atatgcaact catcatcttg cagggtaatc agcccctgac
     2461 gttgcatctc atttgccagc gcatcaataa cgtccggcaa ctcgtcgcga tcccagcgca
     2521 ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc aatacgtcgc
     2581 gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc accaacatat
     2641 gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc agaatgatga
     2701 tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg tgatcgataa
     2761 gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggcacg ttgcgcatca
     2821 gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtgagtgag cgctgacgtg
     2881 atgccagtag cgcagtacag cacaggttca tggcgtttgc cgcgcctgcg ttgttaatgc
     2941 gtaccatcag atcggcagca atattattga ccgtcggtgt taaccatgcc ggacgcaccg
     3001 cttcgatggg atcgatagat tcacgccagt caggtacgtg ctggttaagg taggtcatca
     3061 acggcattgg ttcaccgaaa ttgacgtaac cctgaccgag attacgcagc ttgcttaaac
     3121 cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc gccgcgcagt tctttggcgt
     3181 aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc gtaatcggac
     3241 gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt ttcggatcca
     3301 gcaaacgccc cgtacgggaa cgaccgcctt ctacgaagta ctcgacggaa taaccacggc
     3361 tgaacagttc gccgagatac tcacggaaaa cggtggaata gagcttattg cctttaaaag
     3421 tacggcgaat aaagaacgca cccagacggc ggaaaatcgg cccggccggc cagaagttca
     3481 ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtgatacagc acgtaagaaa
     3541 gcagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca tgaccgtcgt
     3601 gggccaactg gcgaacgcgc tcggcgttat ggacgttgat accctggtaa agtcggttcc
     3661 aggtgaagcc cagaatacgg tcagtcaggc gaatcatctc gtaagagaaa ttcgccgcaa
     3721 tctcttccat cagtgcaatc gcgttctgct gcgctttttc atgggagatt tttttgctgc
     3781 gcgcttcatc ttctaccgct ttcgcaatgg cgcgggaggc gagcagctta ttaaacagat
     3841 cctgacgagc aggcaaacgt gggcctacgg cagccagacg ttgacgggca aagtgcatac
     3901 gcgccacgcg cgccagtttc tgcgcgatag ttttatccgt gccgtgttca tccgccatac
     3961 ggcgcagcga aacggacggt gagaaacgca caaaactgtc gcgaccgagc cacagtacag
     4021 cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gttcacttcg cctttttcac
     4081 gccccggcgc gcgaccaaac atcaccgaca ctggcaccat ctgcacatcc agatttgggt
     4141 tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct ttcggcgtgt
     4201 aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt agcgtgccgt
     4261 cgatttccag cggctctaac gggtcaggca agtcatgtgc cagacactgg gcgcgcaacg
     4321 tcagcaagtc tgctttcgag ttgtacggta aaacgtacat aattggacga gaggtatcca
     4381 gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg atgcttaatg
     4441 gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat gtaaagcctc
     4501 tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat tctgtggtat
     4561 ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac ggtacactga
     4621 tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac tggattcacc
     4681 cgaataatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg gatcaacgaa
     4741 gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc ctgctggctg
     4801 gatgtggacg cgattacccg cgtgctgctt atcagctccg tgatgctggt gatgattgtg
     4861 gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga ataccatgag
     4921 ctttccggac gcgctaaaga tatgggatcc gccgcggtgc tgattgcgat tatcgtcgcc
     4981 gtgattacct ggtgcatcct gttatggtcg cattttggat aacccttcca gaattcgata
     5041 aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt atatactcac
     5101 agcataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca ggcaacaaga
     5161 ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga
     5221 aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct
     5281 ggcacgcaaa ggcgtaattg aaattgtttc cggcgcatca cgcgggattc gtctgttgca
     5341 ggaagaggaa gaagggttgc cactggtagg tcgtgtggct gccggtgaac cgcttctggc
     5401 gcagcagcat attgaaggtc attatcaggt cgatccttcc ttgttcaagc cgaatgctga
     5461 tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatc ggcattatgg atggcgactt
     5521 gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg cacgtattga
     5581 tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac tgttgccaga
     5641 aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca ccattgaagg
     5701 gctggcggtt ggcgttattc gcaacggcga ctggctgtaa catatctctg agaccgcgat
     5761 gccgcctggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct gcatggcatt
     5821 cctcacttca tctgataaag cactttggca tctcgcctta cccatgattt tctccaatat
     5881 caccgttccg ttgctgggac tggtcgatac ggcggttatt ggtcatcttg atagcccggt
     5941 ttatttgggc ggcgtggcgg ttggtgcaac ggcaaccagc tttctcttta tgctgttgct
     6001 gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca aaaatcctca
     6061 ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggctg gggcgttaat
     6121 tgcgctgctg cgtacaccga ttatcgatct ggcgctgcat attgttggcg gtagtgaagc
     6181 agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac cggcgtcgtt
     6241 ggcgaatctg gtattactcg gttggttact cggcgtgcaa tatgcccgtg cgccagtaat
     6301 tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg taatggggct
     6361 gcatatgaac gtgcagggtg cggcgctggc gacggttatt gcggaatatg caacattgct
     6421 gtttggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg gcgaaatgct
     6481 gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg atatcatgct
     6541 gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg cgcgactggg
     6601 gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta ccgcctatgc
     6661 gctggatggt tttgcctacg cggttgaagc acattccggt caggcatacg gtgcgcgcga
     6721 cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga tcgtagcgtt
     6781 actgttttcg gtggtttatt tgctggcagg ggaacacatc attgcgttgc tgacgtcgtt
     6841 aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt tgccgttggt
     6901 tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgtg ccgccgaaat
     6961 gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta cgctgccgtg
     7021 gctgggcaat catggtttgt ggctggcgtt aaccgtcttt ctggcgttgc gcgggctttc
     7081 tctggcggct atctggcggc gtcactggcg caatggtacc tggttttccg caacgtgacg
     7141 gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta atctcacgtt
     7201 cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg attatgaata
     7261 aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag caatggggca
     7321 aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg gtcggtaaaa
     7381 tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg gattgggaaa
     7441 cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa ggatgtacgc
     7501 cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac tgttcaggat
     7561 gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca atcacattat
     7621 gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc acgccttctg
     7681 cacattcttc tttcaccgcg ccgcatcgat cgcaaataaa catagctgac gtatgggtgg
     7741 gctggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta tgcacaaaac
     7801 cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct tgcggttcag
     7861 cttcgcgcag caaatcaagc agatcgtaag cgctgatagc gccatcctgc agactcatca
     7921 ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg cagatttttt
     7981 cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc aaagtggggg
     8041 ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct tattgttgtc
     8101 gtaatgttga ttttctgttt tgtacgtaag gtgttgtttt g
//
LOCUS       DAZKFU020000213         8075 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZKFU020000213 DAZKFU020000000
VERSION     DAZKFU020000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.215"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            255..2492
                     /gene="nrfB"
                     /locus_tag="ACHOQQ_RS19005"
                     /old_locus_tag="pgaptmp_003801"
     CDS             255..2492
                     /gene="nrfB"
                     /locus_tag="ACHOQQ_RS19005"
                     /old_locus_tag="pgaptmp_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGU4472006.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2479..5451
                     /gene="nfrA"
                     /locus_tag="ACHOQQ_RS19010"
                     /old_locus_tag="pgaptmp_003802"
     CDS             2479..5451
                     /gene="nfrA"
                     /locus_tag="ACHOQQ_RS19010"
                     /old_locus_tag="pgaptmp_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGU4472007.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELVLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5452..6342
                     /locus_tag="ACHOQQ_RS19015"
                     /old_locus_tag="pgaptmp_003803"
     CDS             5452..6342
                     /locus_tag="ACHOQQ_RS19015"
                     /old_locus_tag="pgaptmp_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGU4472008.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQRSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6525..7286
                     /gene="envY"
                     /locus_tag="ACHOQQ_RS19020"
                     /old_locus_tag="pgaptmp_003804"
     CDS             6525..7286
                     /gene="envY"
                     /locus_tag="ACHOQQ_RS19020"
                     /old_locus_tag="pgaptmp_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGU4472009.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7332..7408)
                     /locus_tag="ACHOQQ_RS19025"
                     /old_locus_tag="pgaptmp_003805"
     tRNA            complement(7332..7408)
                     /locus_tag="ACHOQQ_RS19025"
                     /old_locus_tag="pgaptmp_003805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7372..7374),aa:Arg,seq:tct)
     gene            7651..>8075
                     /gene="fimZ"
                     /locus_tag="ACHOQQ_RS19030"
                     /old_locus_tag="pgaptmp_003806"
     CDS             7651..>8075
                     /gene="fimZ"
                     /locus_tag="ACHOQQ_RS19030"
                     /old_locus_tag="pgaptmp_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGU4472010.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCST"
BASE COUNT         2001 a         2098 c         2060 g         1916 t
ORIGIN      
        1 ctgctcacct ccaggcactt ccactacatc atgacctgat cggtgccacc actcctcttc
       61 actatgacgt taaccccccc tccttgacac cacgcttttg tctacattct acgcaaacgg
      121 caacttttat aaccagtgta aaaataacgt gccgcaatag tcatacctat taatggtaaa
      181 aagctgtcac aattcataaa aaaccttaat atacgccacc ctaaacataa ccagcgttaa
      241 tgtaaggttt ttgtgtggac tggcttcttg atgtttttgc tacctggctc tacggcttaa
      301 aagtaatcgc gataacgtta gcggtcatca tgttcatcag cgggctggac gattttttta
      361 ttgatgtcgt ctactgggta cgccgcatta aacgcaagtt gagtgtttat cgccgctacc
      421 cgcgaatgag ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg attatggttc
      481 cggcgtggaa tgaaacgggc gtcatcggca atatggccga gctggcggcg accacgctcg
      541 actacgaaaa ctatcatatc tttgttggca cctaccccaa cgaccccgat actcagcgtg
      601 atgttgacga agtgtgcgct cgctttccga acgtgcataa ggtagtctgc gcgcgtcctg
      661 gccccaccag caaagccgac tgtctgaaca acgtgctgga cgccatcacc cagtttgagc
      721 gtagcgccaa tttcgctttt gctggtttta ttctgcatga cgccgaagat gtgatttcac
      781 cgatggaatt gcgtctgttc aactatctgg tcgagcgtaa agatctgatt cagatcccgg
      841 tgtatccgtt tgaacgcgaa tggacgcact tcaccagcat gacttacatt gatgagtttt
      901 cagagctgca tggcaaagat gttccggtgc gtgaagccct cgccggacaa gtgcccagcg
      961 caggcgtcgg cacctgtttc agccgccgcg ccgtgaccgc tctgttagct gacggtgacg
     1021 gtattgcttt cgacgtgcag agtctgactg aagattacga cattggcttc cgcctgaaag
     1081 aaaaaggtat gacggaaatt tttgtccgtt ttccggtggt ggacgaagcc aaagaacgcg
     1141 agcagcgtaa atttttacag cacgcacgga cgtcaaacat gatctgcgtg cgcgaatatt
     1201 tccccgatac cttttcgact gcggttcgac aaaaatcccg ctggatcatc ggcattgttt
     1261 tccagggctt taaaacccat aaatggacct ccagcctgac gctgaactac tttctctggc
     1321 gcgaccgcaa aggggcaatc agtaactttg tcagcttcct cgcgatgctg gtgatgatcc
     1381 agcttttgct gttgctggcg tatgaaagtt tgtggcccga tgcctggcat ttcctttcta
     1441 ttttcagcgg cagcgcatgg ttaatgaccc tgctgtggct aaactttggt ttgatggtta
     1501 accgcatcgt gcagcgggtg attttcgtta ctggctacta cggcctgacg caggggctgc
     1561 tttccgtcct gcgtcttttc tggggcaacc tgattaactt catggccaac tggcgcgcgt
     1621 taaaacaggt acttcaacac ggcgatccac gtcgcgtggc gtgggataaa acaacgcatg
     1681 acttccccag cgtcactggc gatacccgct cgttgcgccc gttaggtcaa attctgctgg
     1741 aaaatcaggt catcactgaa gaacaactcg atacagcact gcgtaatcgc gtcgaaggtc
     1801 tacgcctggg cggttcaatg ctgatgcagg ggctgattag cgccgagcaa ctggcacagg
     1861 cgctggcaga gcaaaacggc gtggcgtggg aatccatcga tgcctggcag atcccttcct
     1921 cgctgattgc cgaaatgccg gcctccgtgg cgctgcatta tgcggtactg ccgctgcgtc
     1981 tggaaaatga tgagttaatt gtcggcagtg aagatggtat tgacccggtt tcgctggcgg
     2041 ccctgacgcg taaagtcgga cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg
     2101 tcacggggtt acgtcactgg tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt
     2161 acaatgcggt tcagcatcag tggctcacgg aacagcaggc cggtgaaatc tggcggcaat
     2221 atgtgccgca tcagttcctg ttcgccgaaa tactgaccac gctcggtcat attaatcgtt
     2281 cagcaattaa cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt
     2341 tggtcaccga aggcgttatc agccaggaaa cgttggatcg cgtcctgaca attcaacgcg
     2401 aattacaagt ttcgatgcaa tcactattac tcaaagcagg tttaaacaca gaacaggttg
     2461 cgcaactgga gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg atggtctggt
     2521 ttactgctga cgtctttatt gagtaccagc gcactcgcag acaatatcgg caccagcgca
     2581 gaagagctgg tgctgagcga ttatcgccat tttgttattt atccccgtct cgataaggcg
     2641 ctgaaggcac agaaaaataa cgacgaagca accgccatcc gcgaatttga atatatacac
     2701 cagcaggtgc cggataatat tccgctgact ttataccttg cggaagccta tcgccatttt
     2761 ggtcatgatg accgggcgcg gctgttgctt gaggatcaac tgaaacgtca cccaggagat
     2821 gcccgacttg agcgcagtct ggcggctatt ccagttgaag tgaaaagcgt tacgacagtt
     2881 gaagaactgc ttgcccagca aaaagcgtgc gatgctgcgc cgaccctgcg ttgtcgcagt
     2941 gaagtcgggc agaatgccct gcggctggca cagttacctg tcgccagagc gcaactgaac
     3001 gatgcgacgt ttgctgcatc gccggaagga aaaacgctgc gaaccgatct gctgcaacgg
     3061 gcaatctacc tgaaacaatg gtcccaggca gatacgctat acaatgaagc acgccagcag
     3121 aacacattaa gcgcggcaga acgccgtcag tggtttgacg tgcttcttgc cgggcagctg
     3181 gacgatcgga tcctggcact gcaatcacag gggatcttca ccgatcctca gtcatatatt
     3241 acttacgcga ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca ttatctcatt
     3301 gaaaataagc cgctgtttac cacggacgca caagagaaaa gttggctcta tctgttatct
     3361 aaatacagcg ccaaccccgt tcaggcattg gcgaattata cggtgcagtt tgccgataac
     3421 cgccagtatg ttgttggcgc gacgctaccg gtgctgttaa aagaaggtca gtacgacgca
     3481 gcgcaaaaac tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg ttatgctgtc
     3541 agcgtggcga cccgtaacaa ggctgaagct ctgcgtctgg cacgcttgct gtatcagcaa
     3601 gaaccggcaa atcttacccg cctggatcaa ctaacctggc aactgatgca gaacgagcag
     3661 tcacgggaag ctgccgattt attgctgcaa cgctatcctt tccagggcga tgcgcgtatc
     3721 agccagactt taatggcgcg actggcgtct ctgctggaaa gtcatcctta cctggcaacg
     3781 ccggcgaagg tggcgatttt atcgaaaccc ttaccgctgg cggagcaacg tcagtggcaa
     3841 agtcagttgc cgggtattgc agataattgc ccggcaatag ttcgcttgct gggcgatatg
     3901 tcgccttcct acgatgccgc cgcctggaac cgtctggcaa agtgttatcg ggacacgcta
     3961 cccggtgtgg cgttgtatgc atggcttcag gccgaacaac gacaaccgaa cgcctggcaa
     4021 catcgtgcgg tagcctatca ggcgtatcag gttgaggact acgccaccgc actggcggcc
     4081 tggcagaaaa tcagtcttca cgacatgagc aatgaggatc tgcttgctgc tgccaatacc
     4141 gcccaggcgg caggaaatgg tgcggctcgc gatcgctggc tacaacaggc agaacaacgt
     4201 ggactgggaa gcaatgccct ctactggtgg ctgcatgcgc aacgttacat tcctggtcag
     4261 ccggaactcg cactgaacga tctcacgcgc tcaatcaata ttgcgccttc tgccaacgct
     4321 tacgttgcgc gggcgacaat ttatcgccaa cgtcataatg tcccggccgc ggtgagtgat
     4381 ttgcgcgccg cgctggaact ggaaccgaat aatagcaaca cccaggcagc gcttggttac
     4441 gccttgtggg atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa
     4501 gggcttccgg acgatccggc actgatccga caactggcct atgtgaacca gcgtctggat
     4561 gacatgcctg cgacgcagca ctacgcccgg ctggtgattg atgacattga taatcaggcg
     4621 ctgataaccc cattgacgcc agagcaaaat caacaacgct tcaatttccg ccgtttgcat
     4681 gaggaggtcg gtcgccgctg gacgttcagt ttcgattctt ctatcggctt gcgttccggc
     4741 gcaatgagta ccgctaacaa taatgtcggc ggcgcagcgc cagggaaaag ctatcgtagc
     4801 tacgggcaac tggaagccga ataccgcatc ggacgcaata tgctgctgga aggcgacctg
     4861 ctctcagttt atagccgcgt ctttgccgat accggagaaa acggggtgat gatgccggtg
     4921 aaaaatccga tgtccggcac cggtctacgc tggaagccgc tgcgcgatca gatctttttc
     4981 ctcgccgtcg aacagcagtt gccgctgaac gggcaaaatg gcgcatccga taccatgctg
     5041 cgcgccagcg cctcattctt taatggcggc aaatacagcg acgaatggca cccgaacggt
     5101 tccggctggt ttgcccaaaa cctgtacctc gatgcggcgc aatatatccg ccaggatatt
     5161 caggcgtgga cggcagatta tcgcgtcagc tggcatcaga aggtagctaa cggacagact
     5221 attgagcctt acgctcacgt tcaggacaac ggctatcgtg ataaaggcac tcagggcgcg
     5281 cagcttggcg gtgtcggggt ccgctggaat atttggaccg gcgagacgca ctacgacgcc
     5341 tggccgcaca aagtcagtct cggcgtcgag tatcaacata cctttaaggc gattaatcaa
     5401 cgtaacggag agcgcaacaa cgcgtttctc accattggag tgcactggta aatgcgtaag
     5461 ttcattttcg tattgctgac actgcttttg gtcagccctt tttcctttgc gatgaaaggt
     5521 attatctggc aaccacaaaa ccgagatagt caggttaccg atacccagtg gcaggggctg
     5581 atgagtcagt tacgtttgca aggtttcgat accctggttt tgcaatggac ccgttacggc
     5641 gatgcattta cccagccaga acagcgcacg ttattgttta agcgtgccgc agctgcgcaa
     5701 caggctggcc tgaagctgat tgtcgggctg aacgccgatc cggaattttt tatgcaccag
     5761 aaacagtcgt ccgcagcgct ggaaagctat cttaatcgcc tgctggctgc cgatctccag
     5821 caagccagat tatggagcgc cgcgcctggc ataacgccgg atggctggta catcagcgcg
     5881 gaaattgacg acctgaactg gcgcagcgaa gccgcccgtc agcctttgct aacatggtta
     5941 aacaacgcgc agcggctgat tagcgatgtt tcagcaaaac cggtttatat cagtagtttt
     6001 ttcgccggaa acatgtcgcc cgatggctat cgccaactgc tggaacacgt taaagcaacc
     6061 ggcgttaatg tctgggtaca ggatggcagc ggcgtggata aactgaccgc tgaacagcgt
     6121 gaacgttatc tacaggccag cgccgattgc caaagatccg ccccagcaag cggcattgtt
     6181 tatgaactgt ttgtcgccgg caaaggcaaa acctttacag cgaaaccgaa accggacgca
     6241 gaaattgcct cgctgttagc gaaacgttcc tcttgcggta aagacactct ctatttctct
     6301 ctgcgctatt tgcccgtcgc gcacggcatt ctcgagtatt aaatctcctc caggtaagtc
     6361 gggtacgacc tggcttacct ctttcgctct cgtcagaatt ccatcaagat taattgctaa
     6421 aaagcgtgct aaagaaagaa gatcgttatc ggcgaacggg ccgatcactc atgcaggaag
     6481 catagatcct ttgagcggag tttctgatat ctgaggagtg cgaaatgcaa ttgagcagca
     6541 gtgaaccttg cgtggtgatc ctgaccgaaa aagaggtaga ggtaagcgtc aataaccatg
     6601 ctacgtttac ccttccgaaa aactacctgg ccgccttcgc gtgcaacaat aacgtcattg
     6661 aactctcaac gttaaatcac gtattaatca cccacatcaa ccgtaacatc atcaacgatt
     6721 atctgttgtt tttaaataag aacttaacct gtgtaaagcc ctggtcgcgg ctggctaccc
     6781 cggttatcgc ttgtcatagc cgtacaccgg aagtgttccg gctagccgcc aaccacagca
     6841 agcagcaacc cagcaaaccc tgcgaagcgg agttgacgcg cgcattgctt tttaccgtat
     6901 tgtctaactt tcttgagcaa tcgcggttta ttgccctact gatgtatatc ttacgcagca
     6961 gcgtccgcga cagcgtctgc cgcattattc aaagcgatat tcagcattac tggaatctgc
     7021 gaattgtcgc cagttcgcta tgtttaagcc ccagcctgct caaaaagaaa ttaaaaaacg
     7081 aaaataccag ctatagccag attgtcacag agtgtcgtat gcgttacgcc gtacagatgt
     7141 tattgatgga taacaaaaat atcactcagg tggcgcaatt atgtggctat agcagcacgt
     7201 cgtactttat ctctgttttt aaggcgtttt acggcctgac accgttgaat tatctcgcca
     7261 aacagcggca aaaagtgatg tggtgaaggg caaaccggaa acggataaga cgggcataaa
     7321 tgaagaagaa atggcgcgcc ctgcaggatt cgaacctgcg gcccacgact tagaaggtcg
     7381 ttgctctatc caactgagct aagggcgcgt tgataccgca atgcggtgta atcgcgtgaa
     7441 ttatacggtc aacccttgct gagtcaatgg cttttgatct ggttgctgaa caagtgaacg
     7501 accgcgtctg attttctgat ttatttcgct atagcggcaa acaaatgcac accgctgcgc
     7561 gtctgaatca agaaaacccg tattttcatg tatcaaagta caatttaccg ccctaacgga
     7621 aaattgtccg ctcctatgag actggtaact atgaaaccaa cgtcggtgat cattatggat
     7681 actcatccta tcatcagaat gtctattgaa gttctgttgc aaaaaaacag tgaattgcag
     7741 attgtcctga aaacggatga ttaccgcata accatcgatt atctccgaac ccgtcctgtt
     7801 gatttaatca ttatggatat agacttgccc ggaacagacg gttttacctt cctgaaaagg
     7861 atcaaacaaa tccagagcac agtgaaagtg ttatttttat catcgaaatc agaatgcttt
     7921 tatgctggca gagcgataca agctggtgct aacggttttg tcagtaaatg caatgatcag
     7981 aatgatattt ttcatgccgt tcagatgatc ctctccggat acactttttt tcccagcgaa
     8041 acgctcaact atataaaaag caataaatgt agtac
//
LOCUS       DAZKFU020000214         8055 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZKFU020000214 DAZKFU020000000
VERSION     DAZKFU020000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.216"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(196..558)
                     /locus_tag="ACHOQQ_RS19035"
                     /old_locus_tag="pgaptmp_003807"
     CDS             complement(196..558)
                     /locus_tag="ACHOQQ_RS19035"
                     /old_locus_tag="pgaptmp_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472011.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITGCVNH"
     gene            complement(733..933)
                     /locus_tag="ACHOQQ_RS19040"
                     /old_locus_tag="pgaptmp_003808"
     CDS             complement(733..933)
                     /locus_tag="ACHOQQ_RS19040"
                     /old_locus_tag="pgaptmp_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472012.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(930..1121)
                     /locus_tag="ACHOQQ_RS19045"
                     /old_locus_tag="pgaptmp_003809"
     CDS             complement(930..1121)
                     /locus_tag="ACHOQQ_RS19045"
                     /old_locus_tag="pgaptmp_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472013.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(1123..1500)
                     /locus_tag="ACHOQQ_RS19050"
                     /old_locus_tag="pgaptmp_003810"
     CDS             complement(1123..1500)
                     /locus_tag="ACHOQQ_RS19050"
                     /old_locus_tag="pgaptmp_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4472014.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(1586..2245)
                     /locus_tag="ACHOQQ_RS19055"
                     /old_locus_tag="pgaptmp_003811"
     CDS             complement(1586..2245)
                     /locus_tag="ACHOQQ_RS19055"
                     /old_locus_tag="pgaptmp_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4472015.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            2585..2878
                     /locus_tag="ACHOQQ_RS19060"
                     /old_locus_tag="pgaptmp_003812"
     CDS             2585..2878
                     /locus_tag="ACHOQQ_RS19060"
                     /old_locus_tag="pgaptmp_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472016.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
     gene            3121..3489
                     /locus_tag="ACHOQQ_RS19065"
                     /old_locus_tag="pgaptmp_003813"
     CDS             3121..3489
                     /locus_tag="ACHOQQ_RS19065"
                     /old_locus_tag="pgaptmp_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472017.1"
                     /translation="MRAELKQVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
     gene            3523..5181
                     /locus_tag="ACHOQQ_RS19070"
                     /old_locus_tag="pgaptmp_003814"
     CDS             3523..5181
                     /locus_tag="ACHOQQ_RS19070"
                     /old_locus_tag="pgaptmp_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU4472018.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            5183..6151
                     /locus_tag="ACHOQQ_RS19075"
                     /old_locus_tag="pgaptmp_003815"
     CDS             5183..6151
                     /locus_tag="ACHOQQ_RS19075"
                     /old_locus_tag="pgaptmp_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HGU4472019.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRIQRRFSALTSLQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            6151..7014
                     /locus_tag="ACHOQQ_RS19080"
                     /old_locus_tag="pgaptmp_003816"
     CDS             6151..7014
                     /locus_tag="ACHOQQ_RS19080"
                     /old_locus_tag="pgaptmp_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4472020.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGID
                     NPAQAIRNARENIKKAASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            7093..7518
                     /locus_tag="ACHOQQ_RS19085"
                     /old_locus_tag="pgaptmp_003817"
     CDS             7093..7518
                     /locus_tag="ACHOQQ_RS19085"
                     /old_locus_tag="pgaptmp_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4472021.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIKLEMDRYVERERSGAQVSQFSGHTGN"
     gene            7718..7873
                     /locus_tag="ACHOQQ_RS19090"
                     /old_locus_tag="pgaptmp_003818"
     CDS             7718..7873
                     /locus_tag="ACHOQQ_RS19090"
                     /old_locus_tag="pgaptmp_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4472022.1"
                     /translation="MKQQKAMLIALIVICLTVILTVLVARKDLCDVRIRTGQTEVAVF
                     VDYESEK"
BASE COUNT         2125 a         1762 c         2061 g         2107 t
ORIGIN      
        1 tctgctgtac aggataatga gcggaaaaga caattcatca tgtttagcgc atctggtgaa
       61 ccaatatcac cgctgtcaat aatcacctgt aacaaagaca ggctttctgt tatctcgcag
      121 tggacattat cgagtacttc aacgcaatta ttcatttttc attatctccg ttattccggg
      181 ggaggagcta tgctgttaat gattaacgca tccggtaata tgcccctctt taatgtaatc
      241 gatgcattca ttcaaaagtt catcaatgat taactttcct gattcagtca gatactcacc
      301 atgttggttg ataccgacag catcctggta tgcagtgcgt attgctgttt caccatcttt
      361 ccgcccaaac tcaccacggg taacgccttc aaatcgtaat agcaactggc ttatgaactg
      421 ttctgttatt tcaatagttg ttaactctcc atcatgacgc tttacagtga ggagattact
      481 tcctgttttt cgtttcatcc gccgcagtgc tgcaaccgtt atgcggcgac gatatgcaga
      541 aatgtattca tctttcattt gttatttccc gtatgccttt ttgagaaaca atactgcaac
      601 tgaccagtat ccggcatcag ccattaataa agcggtttta taggcatgtt tgtttttcat
      661 gtgtcatccc cattttaaaa tagtgagaac tcccgaccgc agaccgtcat gtttttatgt
      721 atgattgtct gtttaatttc ctttattcgt tctcagagca tcaacatact cgtaagcctt
      781 ttcacaggtt ttattcatgg aacgaatcag acaaagtaag aaatcatctg tctctccgtt
      841 atcaccagag tttttaaata taaactcaag cattgacgtg ttttctttta tttctgctgc
      901 cacttcctca agcataatca ggggagtttt catgttcttt gttccttaaa tgcatcgcat
      961 gcgcttctgg cgtattgttg tgccagtaaa aatatgtcat ccgaaagttc atcacattct
     1021 tcatcaccgg aagccgaaat gattaacccc gcttcaagca gtactgcaat gtgatgaaaa
     1081 gctgtttccg gttcgttggt gaggcctttg aacattttca tcttacgatt cctttgtcgt
     1141 gtcagaatgg aaacttgtaa gtaaccacat cggatcgcag tcaagtatat ttgccagagg
     1201 aatcacctgg cttgccagag gttcagcctt gccgttctcc cactggatga tggtttcttc
     1261 atcaactcca agcagcgtgg cgagttcagc ggtggataga ccgcagcttt cgcgttgggt
     1321 gcggatgcca tcacggcaat cttttttgtt tctcggaggt aacagaatat gttttagttg
     1381 ttggattttt tcgattccaa cctcaaggag tacgtgacgg tttctttccg atacagactc
     1441 cagactttca tgaagcagcg attctacttg ttcgataagc atcaattgtg aagtagacat
     1501 aaaaaaacct cgtaaccaat atttcgaaat ctaggttacg agtgagtaat gtaggtgtca
     1561 agagcaatta atgttttttg ttgaattaaa tttgcatacg catctcaacg acaacaccaa
     1621 taatttcaca atccccattg atggggatca gtggatagcg tgggtttagt ggctttagga
     1681 aacattgccc agcgtcttct atgtactgtt taagggttgc ctctccggag tttttgagcc
     1741 tggcgacgac atattttcct gaaaaaactt ctttgtcagg gttaacaaga atgttcatgc
     1801 cttcaaaaat agagaactct ccattctgtg atgtcattga gtcccctttc acttccagcc
     1861 agaagccgtg atcgcccgca tatatatcgc taccaatcca ttcttctgga taagctatgt
     1921 aatcttcgca gttccagggg ccagcactca cccatgttag tttcggatat ctataacggt
     1981 ttggagaaac tggcttagga tttcggacgt tttgaatgct atctgtaggt gatggggcgc
     2041 tgccgtcaag aagccattta ggagagcatt tcaatgcatc cgcgattcta aataggtttg
     2101 aagatcttgt gtcttgtgag tctcctaact ctattttgct gattgttaca cgcgagacac
     2161 cagtcaattt tgccaggtca tcttgtgaca ttcctagctg tactctgcgc attaacagtc
     2221 tgccaccaaa gctatcgtga tccatcttct cccccttctt gtcgaaataa tcgtttcgaa
     2281 acaaatatag caaaaaatct tgaaatctag gtttcggaaa attaagatta atcgaaacaa
     2341 acgtgtcgag gtggatatgg atttgtatga aattctaaaa aacatgtttg gatcgaatgt
     2401 tgaaataggt cgttattttc cccgaagggg aagggctaga accggacagg ctgtaggtaa
     2461 gtggaaaacc cgaggtgtgc cggaagacgt ggcgatcctt tgtcatctgg atcccaaaat
     2521 tccttatcag catccgtcgc tcatgaatgc cagtcatgaa tcgtgatgtt ctgccggagg
     2581 ctgtatgtcc cctgattacg ttcagatgga gatgcctgcc aggtacagcc aggcagattc
     2641 agagtggatt aagcggcagt tacagagcct gccttcctca ctgagacaga aggttgccct
     2701 gaaatacgcg gaggtttacg aagttacttt tgattccgag cctgtttcat tccgtaagga
     2761 gaacagagcg aggcatgaag ccaatgtacg gctgcgcagg ttcgttgaaa cacacgggcg
     2821 tgcattacag gggtatacga ctcagccgcc cctggcagga acgcaacagc gtacctgaat
     2881 ggtgttggtc ttaaaggtga ccgacgggct gagaccccac ttctcaggat gcacctgtgt
     2941 actaccaagc tagtgcataa gttgagaggg ggggtaaggg ggggagtgcc cgtgtgttag
     3001 tgcgaagcac tggaacggga tcttccaaca gacgggtaca gaggttaggt agatctcgat
     3061 ctaaaggggc atccctgaaa aaacggacgt atcagcaaac tagtacatca tggttaaaat
     3121 atgagagctg aactgaaaca ggtactaatc aaccttctgg aagaggaatt cggacatgga
     3181 atggacaagg tcacattcga ctacgttctg caaaagaaaa tcaaatctct gggctgtgag
     3241 ttgcaacgct gtttcactgt caggctgaaa ggtgatcgca ggggatttat cgatcttctg
     3301 gttatttctc ccgatggtca gcgttgcgca gttgaggtgg ataaccgatc gccccgtcag
     3361 cgttcactga tgaaaattcg agcgcttcct gaaggcattt cgggatttgt ttttctccgt
     3421 gatggcaaac atccacagcg ttatatcgcc gatggtgtgg atgtcatccg ggcgacccgg
     3481 tttaagtaat cactgtatca gccctgtcat cgggagaata ccatgctgaa tatcaagccg
     3541 aattttgctc aggaacgtgc gctgaatatg ttgcgtcatg actggaaaac atatcagtcc
     3601 ttcatgatgt acatgcctac agggagtggt aaaaccggcc tggcggcgtt catcactgct
     3661 ggtctggtaa gccgtggcat gcgcgttctg tttgtcgttc cttataccat tttgatcaac
     3721 cagacagcac agcgctttgt tcagtacggg ctttccgggg atgaaatcag ctttatctgg
     3781 cgtgatcatc ccaataacga cccgtcacga ctgattcaga ttgccagtgc ggatacgctg
     3841 attcgccgtg attttcctca gaatatcgac ctgctgatta ttgatgaagc tcatctccgt
     3901 aagcgccgca tcctcacgga aatcgaacga ctggttgcag agggaaatgt gaaggtgatt
     3961 gggttgtccg gtacaccctt ttcatcgttc ctggggcgtt attaccagcg tcttctcaag
     4021 ccgaccacta taggggaact gatccagcgt ggcgaactga gcaattacga attttatgcg
     4081 cccacgaaac cggatctgga aggtgtaaaa acgaaagtct caatggagta cggcagggat
     4141 tacgacgaat cacaactggc agagattatg tgcggcgcgg atctggttgg cgatattgtc
     4201 gataactggc tgtgtaacgg aagggattta ccgacagtgg cgttctgcgt caataaaacg
     4261 catgccagct tcgtgaccat gcagttcaac aaagccggaa ttaacgcaga ggtgatggtt
     4321 gctgaaacgc cacatgatga gcgtcagtta atgatcagtc gttttgaaac gggagcgacg
     4381 aaaatcattg tcagcgttgg cgttctggtg gcgggatttg acagtgatgt ccggtgcatc
     4441 atttacgcac gcccgacaaa atcagagatt cgctggcttc aggcgattgg gagaggattg
     4501 cgtacagcgc caggcaagga gtcatgcctg attttcgacc attccggtac ggttcatcgc
     4561 ctgggatttc ctgaaaatat tgagtatgac gaactgcccg gcaaaaacga cgggatgcag
     4621 gtgtcatcat cacgtggtaa cgaaattcgg gaagaaaaac tgccgaaaga atgtccgggg
     4681 tgtcatttca tgaaaccggc aggtgtttat gtctgtccga aatgcggatt taaaccgctg
     4741 gctggtgagg atgtgaacac agatacccgc cgcaatatca ggaaactcag caaggacgaa
     4801 aaaatttaca ccaaaagcga caaacaggcc tggtggagtc agatcaaatt ctatcagcgt
     4861 cagcgtgcct ctctggggcg accggtcagc gatggatggt gcgctcacac tttccgggag
     4921 aagttcggcg aatggcctga cgggctgagc agttttccga tggaaatcac cccggaggtc
     4981 agtaattaca tcagacacaa attcatccgg tttgccagag ggcgtagccg ggcagagaag
     5041 atgacggaaa aatcccccga agcgctttct ctgtccctgc ttcatggtgt ccggtctggg
     5101 gtgccggaag gcagtgagtc gtggcagatc atgcaggcaa agcaacaact tcagaaaaac
     5161 agaaacagtc tgagtcagta agatgaaaac agcagaagca gcgaaaggcc actggcctga
     5221 aattttaaaa cattacggac tgccgccagt cacgggtaaa aagcacttca aaggggaatg
     5281 tccggtttgt ggtgccagag gtaagttccg tattgatgac cgtgatggca ccggaacgtg
     5341 gatctgtgtt tgtggtagcg gtgacggcat gaaactgatc gctctgacac aaaaaagagc
     5401 gtttcatgaa atttgcgctg aaatagaccg catcacgggg aatgaatacc ggagagataa
     5461 accgcctgtg atctgtacgt cggaaagcct gagatcgcgc attcagcgtc gattttcagc
     5521 gctcacgtcg ctacagggaa catctggtgc tgaatatctt cgttcccgtg gcatattcag
     5581 tcttccggtt gagggagttc gcttcaacag ccagcaaaat tataacgggc gcatgttcca
     5641 gtctctttat gccctggcga ccgacgacaa gggagaactg tgttacctgc atcagacatt
     5701 gcttgatgga gctaaaaaag cagacattgg tatcagcgcc agaaggctca aatctttaca
     5761 ggacgataac tatctggacc atgcccggtc agttgctatc aggatgtttc cggtagccag
     5821 tacactgggt atagctgaag gtatcgaaac cgcgttgtcc gcacatcaga tttacaaagt
     5881 aaatacctgg gcaaccatta acagtggttt tatgaaaaag ttccgcgtac cggcgggagt
     5941 cacccatctg attatttttg ccgatcgcga tgaatacagt gctaccggaa tggctgcggc
     6001 ctgtgaatgc gcacatgcaa atctccaggc ccgaaatgac attcagcggg taagtgtccg
     6061 gtggcctgat catgatgatt tcaacaatat gctcatgaac ggcgatcagg ttcgggaact
     6121 ggttttttac agaaaaaagg cggttgcgtg atgcgtacgg acaatcaggt acataaagct
     6181 ttattcacta tcccgacggc agcgtacagc gccgttccgg caaatatcaa acctctgcca
     6241 gaacaaagga ggatcaccgg acataaacag acagatgctt atctctggat tctggaggtt
     6301 attcacctga acgaggccgt acatctggac gcagccgaag cagcactgga gaaactcaaa
     6361 ataacgccgg aagaagcgag tgaacggtat ggacgttatt tgcaggagat taatgttgat
     6421 cctttccaga ttgctttcgc aaccataggc atagataacc cggcgcaggc gatcaggaat
     6481 gcccgtgaga atatcaaaaa agccgcatca gtcagggcca catttggcag ctatgaggca
     6541 gcactcgatg atgtggaggc tgagcggata attcgcacct ccccgaaatt tatcgacgat
     6601 tactactggg gctggactgc ggcagagaaa aaagccggaa gtattgacgg tgttcgctca
     6661 aatgaaattg atgatcagcg tcgtgcatat gttgatggct atcgtgatgt actgccagag
     6721 cctcatacat tgtcagacgt tgttcgtgag tttatttact gggactggct ttatgagatg
     6781 cgccagacag cggggagaga aacaggggac aaatacggtt tcacaggtga acatcatgag
     6841 tcagtatatg atcggcagtt ctggctcgaa aatttgctgg gaaaaataaa gcctgtgacg
     6901 cgtgatgaag cggttgaagt gtgtcgctgg tttctggcaa gcggaaaaga tgagtacatg
     6961 gaagacaatg gttcagcggt cattctcaat ctggttgggg agtgtgagca atgaagccgg
     7021 agatatccag tagacatttt ccccctgagc attcaaatca gaaaaagtat ggttacagcg
     7081 gagtacagaa taatgcgtga tatccaacag gttctgtccc gatggggtgc atgggtggta
     7141 aataatcatg agagtgtggc atggtccggc gtcgctgccg gatttaaggg agtgatccca
     7201 tcaaaggtta aatcacggcc tcagtgtacc gatgatgatg cgctgattat cagcaattgc
     7261 atggcgcaac tgaacgtcaa caacagcgat ttgcatgatt ttctgtatga ttattatgtg
     7321 ttcgggatga cgcttatggg actgggccgt aagcatgagc gctctgattg ctgggccggg
     7381 cgggtactgc aaaaagctga aggtgttatt gaggggatgt tgattatgca gggaataaaa
     7441 ctggaaatgg acagatacgt tgagcgtgaa cgatcagggg cacaggtcag tcagttttcc
     7501 ggacatacgg gaaattgaaa gcgcggggtt ttactgtaga atgactgcgg gtgcttgagg
     7561 gtgtctgcct cgggcatgct gccgtaaggc agacggagaa aagccccagt taacattacg
     7621 cgtcctgcaa gacgcttaac attaatctga ggccaatttc atgctgaaca catgtaggtt
     7681 agcctcttac gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa aggcgatgtt
     7741 aatcgccctg atcgtcatct gtttaaccgt cattctgacg gtactggtag cgaggaaaga
     7801 cctctgcgat gtacgaatcc gaaccggcca gacggaggtc gctgtcttcg tagactacga
     7861 atctgagaag taagagacct ggcgggggag taatctcccg ccccctctga tgtgtcaggc
     7921 atcctcaacg cacccgtact ttagttgtca tatgctccgc aaaatgcagg ttgtcgttgc
     7981 aacccgtgcg gtaatttttt gtgtgcgagt gctcaaaaaa cgttgatttt cataaaagga
     8041 cgattttatg ctgcc
//
LOCUS       DAZKFU020000215         7919 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZKFU020000215 DAZKFU020000000
VERSION     DAZKFU020000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.217"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..1096
                     /gene="ompN"
                     /locus_tag="ACHOQQ_RS19095"
                     /old_locus_tag="pgaptmp_003819"
     CDS             2..1096
                     /gene="ompN"
                     /locus_tag="ACHOQQ_RS19095"
                     /old_locus_tag="pgaptmp_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HGU4472023.1"
                     /translation="MAAGAAHAAEVYNKDGNKLDLYGKVDGLHYFSDNSAKDGDQSYA
                     RLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGLKFADYGSFDYGRN
                     YGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFFGLVNGLNFAVQYQ
                     GNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAYTSSDRTNDQVNHT
                     AAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVANKTQNFEVTAQYQF
                     DFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATYYFNKNMSTYVDYK
                     INLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1237..1671
                     /gene="uspF"
                     /locus_tag="ACHOQQ_RS19100"
                     /old_locus_tag="pgaptmp_003820"
     CDS             1237..1671
                     /gene="uspF"
                     /locus_tag="ACHOQQ_RS19100"
                     /old_locus_tag="pgaptmp_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGU4472024.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1847..2782
                     /gene="ttcA"
                     /locus_tag="ACHOQQ_RS19105"
                     /old_locus_tag="pgaptmp_003821"
     CDS             1847..2782
                     /gene="ttcA"
                     /locus_tag="ACHOQQ_RS19105"
                     /old_locus_tag="pgaptmp_003821"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGU4472025.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2911..4284)
                     /gene="dbpA"
                     /locus_tag="ACHOQQ_RS19110"
                     /old_locus_tag="pgaptmp_003822"
     CDS             complement(2911..4284)
                     /gene="dbpA"
                     /locus_tag="ACHOQQ_RS19110"
                     /old_locus_tag="pgaptmp_003822"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGU4472026.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQQLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGYIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4314..4487)
                     /gene="ynaL"
                     /locus_tag="ACHOQQ_RS19115"
                     /old_locus_tag="pgaptmp_003823"
     CDS             complement(4314..4487)
                     /gene="ynaL"
                     /locus_tag="ACHOQQ_RS19115"
                     /old_locus_tag="pgaptmp_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGU4472027.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4762..5745)
                     /gene="zntB"
                     /locus_tag="ACHOQQ_RS19120"
                     /old_locus_tag="pgaptmp_003824"
     CDS             complement(4762..5745)
                     /gene="zntB"
                     /locus_tag="ACHOQQ_RS19120"
                     /old_locus_tag="pgaptmp_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGU4472028.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5759..5806)
                     /gene="ydaN"
                     /locus_tag="ACHOQQ_RS19125"
                     /old_locus_tag="pgaptmp_003825"
     CDS             complement(5759..5806)
                     /gene="ydaN"
                     /locus_tag="ACHOQQ_RS19125"
                     /old_locus_tag="pgaptmp_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGU4472029.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            5999..7231
                     /gene="dgcM"
                     /locus_tag="ACHOQQ_RS19130"
                     /old_locus_tag="pgaptmp_003826"
     CDS             5999..7231
                     /gene="dgcM"
                     /locus_tag="ACHOQQ_RS19130"
                     /old_locus_tag="pgaptmp_003826"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGU4472030.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLVA"
     gene            complement(7252..7815)
                     /gene="smrA"
                     /locus_tag="ACHOQQ_RS19135"
                     /old_locus_tag="pgaptmp_003827"
     CDS             complement(7252..7815)
                     /gene="smrA"
                     /locus_tag="ACHOQQ_RS19135"
                     /old_locus_tag="pgaptmp_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGU4472031.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         2085 a         1998 c         1876 g         1960 t
ORIGIN      
        1 gatcgccgca ggtgctgcac atgcagccga agtttataat aaagacggca acaaattaga
       61 tctgtatggc aaagttgatg gcctgcatta tttttctgat aattcagcga aagatggcga
      121 ccagagctat gcgcgtctgg gttttaaagg cgaaacccaa attaacgatc aacttactgg
      181 ctacggtcaa tgggaataca atattcaggc aaacaacact gaatcttcaa aaaaccagtc
      241 atggacccgt ctggcatttg ccggactgaa atttgcagat tacggttctt tcgattacgg
      301 acgtaattat ggcgtaatgt acgacatcga aggctggacc gatatgctgc ctgaatttgg
      361 cggtgactct tataccaatg cagacaactt tatgactggt cgtgccaatg gcgtcgcgac
      421 ttatcgtaat actgatttct tcggtctggt aaatggtctg aacttcgcgg tgcagtatca
      481 aggtaacaac gaaggtgcca gtaatggtca ggaaggcacc aacaacggac gtgatgttcg
      541 ccatgaaaac ggtgacggct ggggtctttc cacaacatat gatttaggca tgggctttag
      601 cgctggtgcg gcatacacct cttctgaccg caccaatgac caggttaacc atactgcggc
      661 gggtggtgat aaagcagacg cgtggactgc tgggctaaaa tacgatgcta acaatattta
      721 cctggcaacc atgtattcag aaacgcgtaa tatgaccccg tttggcgaca gcgattatgc
      781 agttgcgaac aaaacccaga actttgaagt cactgcacag taccagtttg attttggtct
      841 gcgtccggca gtctctttcc tgatgtctaa aggccgtgac ctgcacgctg ccggtggtgc
      901 agacaacccg gcaggtgttg atgataaaga tctggttaaa tacgccgatg ttggcgcgac
      961 ttactatttc aataaaaaca tgtccactta tgttgactat aaaatcaacc tgttggatga
     1021 agatgacagt ttctacgctg ccaacggcat ctctaccgat gatattgtcg ctttaggtct
     1081 ggtttatcag ttctaaatcc tcctgcccgc tgttatggcg ggctttttct gcttattctt
     1141 cctcctttga tctaaattaa aaatgtgaac tccgtcatta cacaaaaagt gtcatctggc
     1201 gttacacttt atgcggatac taaaacagga ggttttatga acagaacgat tcttgtccct
     1261 atcgatattt ccgattcaga attaactcaa cgcgtgatta gccacgttga ggcagaggca
     1321 aagattgatg atgcagaggt tcatttcctg acggtaatac cttcactgcc ctactatgcc
     1381 tctctgggtt tagcgtattc cgcagaatta ccggcaatgg atgacctgaa agcggaagcc
     1441 aaatcgcaac tggaagagat cattaaaaaa tttaaactgc caaccgacag agtgcatgtc
     1501 catgttgagg aaggttcgcc caaagaccgt attctggaat tggcgaagaa gatccccgct
     1561 catatgatca tcattgcttc ccatcgaccg gatatcacca cttatctgct cggttccaac
     1621 gccgcagctg tagtgcgtca cgcagagtgc tccgtgctgg ttgtgcgctg acactaacgc
     1681 ccgcacattg ctgcgggctt tttgattcat ttcgcaaatg tgctgacatt ttcccctcta
     1741 atccgtacca tacacgccac agtttttata tcagatttct tatgctgggc gttccggcat
     1801 gacgcatact cttctgatgc catataacga attgagtcgc ttttaaatgt cgcaaaatca
     1861 agaaattagc aagaaagaac aatacaatct gaacaaatta caaaaacgcc tgcgtcgtaa
     1921 cgtgggcgaa gccattgctg acttcaatat gattgaagaa ggcgatcgca tcatggtttg
     1981 cctctccggg ggtaaagaca gctataccat gctggagatt ctgcgcaatt tgcagcaaag
     2041 cgcgccaatc aatttttcgc tggtggctgt taacctcgat caaaagcaac cgggcttccc
     2101 ggaacacgtt ctgcccgagt atcttgaaaa gctgggcgtt gagtacaaga ttgttgaaga
     2161 gaacacttac ggtatcgtga aagagaagat tccagagggc aaaaccactt gctcactgtg
     2221 ttctcgactt cgtcgcggta tcctttatcg caccgcaacg gaactggggg cgacgaagat
     2281 cgcgttgggc caccatcgtg acgatatcct gcaaacgttg ttcttaaata tgttctacgg
     2341 cggcaagatg aaaggtatgc ctccgaaact gatgagcgat gatggcaaac atatcgttat
     2401 tcgtccgctg gcctactgcc gcgagaaaga tattcagcga tttgccgatg caaaagcgtt
     2461 cccgattatt ccgtgcaacc tgtgcggttc acagcctaac ctgcaacgtc aggtgattgc
     2521 tgacatgttg cgtgactggg ataaacgtta tccagggcgt atcgagacga tgttcagcgc
     2581 gatgcagaat gtggtgccgt cgcatctgtg cgataccaac ctgttcgatt tcaaaggcat
     2641 cacccacggt tctgaagtgg ttaacggggg tgatctggcg tttgatcgcg aagagatccc
     2701 actacaaccg gcgggctggc agccagaaga agatgaaaat cagttggatg agttacggct
     2761 gaatgtggtt gaagtgaaat aaccaggata gcgcccgatg cgcaagcgta tcgggctact
     2821 cttatggagg ccggataaga cgcagccagc gtcgcatccg gcaatcccga ataagatgtt
     2881 tactcttgca cccggcaatt caacatttca ttattttaat aaccgcaccc ggcacgtttt
     2941 tcctttaatc ttcccgccct gtaactgttt ccatgcttta tgagcaacag cctgacggac
     3001 cgcgacatag acatgcgccg gatgcacggc gattttgcca atatctgcgc catcaagccc
     3061 gatatatcct gtcagtgcac ctaatacatc acccgggcgc attttggctt ttttcccgcc
     3121 atcgatacac aacgttgcca tttctgcttc cagcgtcaca atggaactat tagctggcgg
     3181 cgtttgccag ttaagtttta tctgcaacat gtcagaaatg atattggccc gctgtgcttc
     3241 ttccggagca cagaaactga tcgccagacc gctatttcct gcacgagctg tacgaccgat
     3301 gcgatgtaca tgaacttcag ggtcccacgc cagctcaaag ttcaccacca gctcaagcga
     3361 tttaatatcc agaccacgcg cagcaacatc agtcgcgacc agtacacggg cgctaccgtt
     3421 agcaaaacgt accagggtct gatcgcgatc gcgttgctcc aaatcgccgt gtaatgacaa
     3481 tgcactttgc cctacttcat tcagcgcgtc gcagacagcc tggcaatctt ttttggtatt
     3541 gcaaaacacc acgcaagagg atggctgatg caagcttaat aactgttgca acagaggaat
     3601 tttgccttta ctggatgtct cataaaattg ttgttcaatg ggtggcaaag catctgttga
     3661 gtcaatttca atcgccaaag gatcgcgttg cactcgtccg ctgattgcgg cgatggcttc
     3721 cggccaggtt gccgaaaaca gaagcgtctg tcgagatgcc ggcgcaaaac ggatgacatc
     3781 atcaatggca tcgctaaatc ccatatccag catgcggtcg gcctcatcca tcaccaaagt
     3841 attcagcgca tccagtgata ccgtgccttt ttgcaggtga tccagcaaac ggcccggcgt
     3901 tgccacgata atatgcggcg catgttgcaa cgaatcacgc tgcataccga acggttgacc
     3961 accgcacaac gtcaaaattt tggtatttgg cagaaaacgc gccagccgac gcaattcacc
     4021 tgccacctga tccgccagtt cacgcgtagg acacagcact aaagcctggg tttgaaatag
     4081 cgacgcatca atttgctgta acaagccgag gccaaaagcc gccgttttgc cgctgccagt
     4141 tttcgcctgc acgcgaacat cttttccggc aaggatcgcc ggaagcgcgg cggcctgcac
     4201 cggcgtcatg gttaaataac ccaactcatt aaggttcgtg agttgggcgg gaggcaaaac
     4261 attcagggta gaaaaagcgg tcacaatcta ttctcgtggt catcgacgca aagttagcag
     4321 gcgcgtatcc tcgcagatct acgctcacga tgcgacaatt taatcggttc ttcatcgggt
     4381 ggtgggtcag gcatgggttg cgggcgaggg atcggatcgg gcactggaac aggatcgcca
     4441 ggaatcggtt cagggacagg aatttgcaaa taaataagtg tcgtcatatt tccctccggt
     4501 cattgggtgg actcttaaag ggtagacgct gataaataac aggcaaaaaa aagccgactc
     4561 atcaaagtcg gcgtcgtacg aatcaattgt gctatgcagt aattcaaaaa aggaagtaag
     4621 acaatatgga gcgcaacgcc catcgcttga cgttgcattc acctgcaaga gagatattgc
     4681 cctgaatggg tagagagttt attgacttcg ctcaaacttt gcggcgtttt tgtatacaga
     4741 tagccggaaa aattgctttt gttacaacca tttactacga tgcaaccata aagcaacacc
     4801 accaataaga acaactaaca gaatacaaaa aattgaaaat ccgaattgcc acccgccgcc
     4861 agggatccca ccaaggttga cgccaaataa ccctgtcaga aaggtactgg gtaaaaagac
     4921 cattgccatc aatgacattg tataggtacg acgagctaaa ttttcctgca tcacctgagc
     4981 gatttcatcc gccatcacac cagtccgtgc tatacaggcg tcgatttcgt caaggccgcg
     5041 cccaaggcga tcggcaatat cctgcatccg acggcgttgg tcatcgctca tccacggcaa
     5101 acgttcactg gcaagacgag catagacatc acgttgcggt gccatatagc gacgcatcac
     5161 aattaattgt ttgcgcagca gagccaggaa tccacgcggc ggaatttgct gatcaaggag
     5221 attatcttca aggtcgataa ttttatcgtg cagctgctcg ataaattcgc tggaatgatc
     5281 ggtcaacgca tcgcacacat ccaccagcca ccctccgcaa tcggtcggac ccgtgccctc
     5341 ttccagatcg ctcaccacat cgtccagcgc caacactttg cgttgtcggg tcgaaacaat
     5401 taaccgcccg tccatatata cacgcatggc gaccagttga tcggggcgtt catcggtgct
     5461 gccgtttata cagcgcaatg taatcagcgt gccttcaccg agacggctga ctcggggccg
     5521 tgtgctctca cctgccagcg catcacgtac gttattggga agcagcggtg ttgtcgccag
     5581 ccattgggcg ctatcatggt gtacataatt aaggtggagc cagcagggat gcgcttcatc
     5641 aatcacatct gtattttcca gcggtttaac gccgcctcta ccatccagca tccaggcaaa
     5701 tactgcatcc gggacattaa cgtccgatcc cttaatcgct tccacagtgc ctccatcatc
     5761 aacgcattat tttgtagtct agccttctgg ccctgttacg caacatctca tcaccccatt
     5821 accctgaaat gattaataaa attctgtcta aattgaatac aaaaagcaaa atgcttttcc
     5881 gtatacaaac cgtgtgaagt gttaaatagc gtctatcatt atcagaatta tctgatcata
     5941 tgacgtgctt ttttgcgatc ggatagcaac aaaaattgat aaaaataacg ggatctcaat
     6001 gattacgcac aacttcaata ccctggactt actcaccagt cctgtctgga tcgtttcgcc
     6061 ctttgaggaa cagttaattt atgccaatag cgcggcgcga ctgttgatgc aagacctcac
     6121 gtttagtcag ctacgaaccg gtccctattc cgtctcctca caaaaagaac tgccgaaata
     6181 cctctccgat ctgcaaaacc aacacgatat tatcgaaatc ctcactgttc agcgtaaaga
     6241 agaggaaaca gcattaagct gtcggcttgt tttgcgagag ctgacagaaa cagaaccggt
     6301 gattattttc gaaggtatcg aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc
     6361 aaattatcag cgcaaaaaac aaggttttta tgcgcgcttt tttctgacta actctgcacc
     6421 aatgttgttg attgacccgt cacgagatgg ccaaatcgtc gatgctaacc tcgccgcgct
     6481 caatttctat ggttataacc atgaaacgat gtgccagaaa catacctggg aaataaatat
     6541 gctcgggcgt cgcgtcatgc ctatcatgca tgaaatttcg catttacccg gcggtcataa
     6601 acctttgaat tttgttcata aactggcgga tggatctact cgtcatgtgc agacctatgc
     6661 cggaccgatt gaaatttatg gcgacaagct catgttatgt attgtgcatg atattactga
     6721 gcaaaaacgg ctggaggagc agctggaaca tgctgctcac catgacgcga tgaccggatt
     6781 actgaatcgg cgacagtttt atcacattac ggaaccaggc caaatgcagc atctcgccat
     6841 cgctcaggat tacagcttgt tgctcatcga caccgatcgt tttaaacaca ttaacgatct
     6901 ctatgggcat tctaaaggtg atgaggtgtt atgcgccctc gcccgcaccc tcgaaagttg
     6961 cgctcgcaaa ggcgatttgg tgtttcgttg gggaggcgaa gagtttgtct tattgctacc
     7021 aagaacccca ctggataccg cgctttcgct ggctgaaact atccgcgtaa gcgtggcaaa
     7081 agtgagtatt tcgggcttac cacgctttac cgtcagcatt ggtgtggcgc atcacgaagg
     7141 aaatgaaagc atcgatgaac tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa
     7201 tgatggtcgc aaccgcgtgc tggtggcata agccgcggat gcgtctcgag atcaacgact
     7261 gcgcttagcg tggcgctccc agttttcttg cttcgcctgc gccgttttac gtagtgcgac
     7321 gtaacacgcc ccgctgccgc catgatgcgg tagcgcggtg caatatgcct gaacatcatc
     7381 aaattcggtc agccagcgcg ccacatagct gcggacaata ttggcatgcg atttatcatc
     7441 ccgcccttta ccatgaataa tcagcacgtt acgcaaacca tccgccaggg cttgttgaat
     7501 aaaactgaac accattttgc ggcactcttc caccggctgg cgcaaaagat tcaggctcgc
     7561 ctgttgcgga tatttaccac tacgcagctt atccagcacc ccatgttgca acccttcccg
     7621 ccgaaactcc agcggctgac ttagcgggat aatgtcgaga aatccggtag tgaggaaatt
     7681 atcaagctgc agcgtgtcga tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg
     7741 gacatcggta gcacgtttca gcggctggac atcttccatg gcgtcaagaa acagcgattt
     7801 gtcgtcaagg ttcatgttac atcctcccgc aattaagagc gcgatatgat aaccagaccg
     7861 ggtcggtcca acaacgtatt acccaaaatt ccagtaataa gttccaaata ttgccgata
//
LOCUS       DAZKFU020000216         7882 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZKFU020000216 DAZKFU020000000
VERSION     DAZKFU020000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.218"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..417
                     /locus_tag="ACHOQQ_RS19140"
                     /old_locus_tag="pgaptmp_003828"
     CDS             <1..417
                     /locus_tag="ACHOQQ_RS19140"
                     /old_locus_tag="pgaptmp_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HGU4472032.1"
                     /translation="DPGAQGPKGETGATGPAGPRGAQGPKGEKGDPGPRGERGETGPM
                     GPPGASDGKSRVVGIRLGNKQTYAPQTDSTTWAVDLDIGAMITGIGGYNDGNKTLIDR
                     VSYRPLQVTFDGSQWRTVSVGEYVSSGASGFEYFPL"
     gene            459..1130
                     /locus_tag="ACHOQQ_RS19145"
                     /old_locus_tag="pgaptmp_003829"
     CDS             459..1130
                     /locus_tag="ACHOQQ_RS19145"
                     /old_locus_tag="pgaptmp_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4472033.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGIGRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTAEEIIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
     gene            1668..3293
                     /locus_tag="ACHOQQ_RS19150"
                     /old_locus_tag="pgaptmp_003830"
     CDS             1668..3293
                     /locus_tag="ACHOQQ_RS19150"
                     /old_locus_tag="pgaptmp_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472034.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSELPI"
     gene            3290..4558
                     /locus_tag="ACHOQQ_RS19155"
                     /old_locus_tag="pgaptmp_003831"
     CDS             3290..4558
                     /locus_tag="ACHOQQ_RS19155"
                     /old_locus_tag="pgaptmp_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4472035.1"
                     /translation="MSKKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            4573..4851
                     /locus_tag="ACHOQQ_RS19160"
                     /old_locus_tag="pgaptmp_003832"
     CDS             4573..4851
                     /locus_tag="ACHOQQ_RS19160"
                     /old_locus_tag="pgaptmp_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472036.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            4857..5474
                     /locus_tag="ACHOQQ_RS19165"
                     /old_locus_tag="pgaptmp_003833"
     CDS             4857..5474
                     /locus_tag="ACHOQQ_RS19165"
                     /old_locus_tag="pgaptmp_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472037.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            5565..6299
                     /locus_tag="ACHOQQ_RS19170"
                     /old_locus_tag="pgaptmp_003834"
     CDS             5565..6299
                     /locus_tag="ACHOQQ_RS19170"
                     /old_locus_tag="pgaptmp_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4472038.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHSRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            6530..6670
                     /locus_tag="ACHOQQ_RS19175"
                     /old_locus_tag="pgaptmp_003835"
     CDS             6530..6670
                     /locus_tag="ACHOQQ_RS19175"
                     /old_locus_tag="pgaptmp_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4472039.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            6727..7128
                     /locus_tag="ACHOQQ_RS19180"
                     /old_locus_tag="pgaptmp_003836"
     CDS             6727..7128
                     /locus_tag="ACHOQQ_RS19180"
                     /old_locus_tag="pgaptmp_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472040.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            7222..7872
                     /locus_tag="ACHOQQ_RS19185"
                     /old_locus_tag="pgaptmp_003837"
     CDS             7222..7872
                     /locus_tag="ACHOQQ_RS19185"
                     /old_locus_tag="pgaptmp_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472041.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYENIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGGAKTPGTGANAIQHQAQNWRL"
BASE COUNT         2042 a         1620 c         2269 g         1951 t
ORIGIN      
        1 gaccccggag cacagggtcc gaaaggtgaa acgggagcaa caggtcccgc tggtccgcgg
       61 ggagcgcagg gaccgaaagg ggagaaaggc gaccccggtc ccagaggtga gcgtggagaa
      121 accgggccga tggggccgcc cggggcatca gacgggaaga gccgggttgt gggtatcagg
      181 ctgggtaata aacagactta tgcgccgcaa acagacagca ctacatgggc agtggatttg
      241 gatattggtg cgatgattac ggggattggc ggttacaacg acggaaacaa aacccttatt
      301 gacagggtat cttacaggcc gcttcaggtg acatttgatg gttctcagtg gcgtacagtc
      361 agcgtgggtg agtatgtgtc gtcaggagct tcggggtttg agtattttcc attataaagc
      421 gcatcatctt tcagacatgt aaccgggagg actgacagat gcacataaag aatttcagac
      481 aatacacgcc ggaaaatccg gatgtgccgg gtgcgatgta cctgaaatca gaggatggtc
      541 aggactggta tgagtgtcag tcgttatttt cagcagagac gctgaaggtg gtttataaca
      601 gtgccggagt cattaccggc atcggcaggg tggcatcagt tctgtggcca gtgggccaga
      661 gtgtggtgga ggtggcagat acggaagaaa accgcaaagc tgacatctcg gggcgctggg
      721 gttttgacgg ggagaaaatc acggacctgc tgaccgcgga gaaagcgcgc gggatgaagg
      781 gcgatgaaat taacgcctgg cgtaatgcga tggaagcggc gaactacacg tttgagcaca
      841 atgggcgtaa atgggactac gggaagtcaa cgcagacgcg tcttgagccg tcggtggcgg
      901 cagcgaaagc ggggaaactg ccggaggcgt ttttctggac ggatgcggaa aacaatgatg
      961 tgccggtgac agcagaagag attattgcgc tgagtgaagc ggcagagcag gcgatgttca
     1021 ccaaagggat ggaaatccac gttcgccagc gcaccatgaa gaaagagctg gaaaagctga
     1081 ccagtgcgga tgagatactg gcatacaggg ttggttgggc acaggaataa cagacaaaaa
     1141 gatgggggac catcacctcc cccatgaact gagtcaatta tacacttgtg aagtatttac
     1201 tatgcgaata aatactgtca cggaaagtgc aggcctggat gtgatggaaa tcatgtttcc
     1261 ggcgcatcct gttgatatgg ataatttttc agatttccgg aagtgagagt ttttggtgtt
     1321 tccataccag ccacagttga tgtgtgttgt ggcgcctggc agtgcgatta aaggagcaga
     1381 gggaataaaa aacgggggca tgaccaaccc ccggtacaag gaaggagtaa ctacaatgga
     1441 aaaattaatg aagaccttta gtgctacgtt atatggcgaa taaagactgc cacagggaag
     1501 tttgcagaaa cagtgatgcc gatcgtgttt ctgacgctct tctctgataa gagtaatttt
     1561 tcagatttct ggaaatctgt attctgcaca ggcgcaattg tatgccgcct ttaaaacttc
     1621 aattcagtga ctcacctgcc attcaaattt tcggatacca gacaaccatg ccttatatcg
     1681 atataacaac tatgcgcggg atgatgccag gcgttattgc atctatgctg ccagatcatt
     1741 ctgctgtact ggcagaaaac tgtcattttc gctatggagt gatcacgcct gaacaccaga
     1801 tgtcagaggc tgagaaaaca ttcgcgatta agccgaaaac catttttcat taccgtgacg
     1861 atttctggtt tgcatggacg gatgtggtgg atgtgatccg cagtccggtc gctcaggact
     1921 cccacgggcg tatttactac actgacgggc gttttcctaa agtgacggat gcgaccattg
     1981 ccacaaaagg ggacgggaat cacccgacat catcgtatcg tctggggatc cccgcgccga
     2041 cgacagcacc tgtctgtact gttcagcagg gcggtgatgt ttctgacgat aacccgaatg
     2101 atgacgaaac ccggttttat acggaaacct ttgtctcaga ttatggtgaa gaaggtccgc
     2161 caggtccggc gtctctggag gtaacactcc gtactccggg gactgcggta cagctgacgc
     2221 tgtctccggt gccattgcag aatgccagta ttaaacgccg ccggatttat cgctctgcat
     2281 caggtggagg agaagcggat tttttacttg tggctgaact ggatgcatcc gtgctcagtt
     2341 acacggacaa aataccgggg aaaaaccttg gaccttctct ggctacatgg gattacctgc
     2401 cgccgccaga gaatatgaca ggcctttgcc tgatggctaa tggtattgcc gccgggtttg
     2461 ccggtaatga agtgatgttt tcggaagcgt atctgccgta tgcatggccg gaagtgaatc
     2521 gtcacacgac ggcagaagat attgtggcta tctgtccgct gggaacgtca ctggtggtgg
     2581 cgacaaaggg ggagccttat ctgttcagtg gtgtatcgcc ttccaccatt tctggctcca
     2641 gaattccttc catgcaggca tgcctgagcc gaagaagtat ggtggcgatg gagggattcg
     2701 tactctatgc cgggacaaac ggtctggtat ctgttgatgt aaacggtaat acagcactgg
     2761 caacggaaaa gattatttca cctgaacagt ggcagagtca gtttaacccg gcgtccattg
     2821 tggcttattc ctggcgtggt gagtacattg cctgttacac gaaaccggat ggtaagcagg
     2881 atgtgtttgt attcagtccg gtgaacatgg atatccgtta tctcagtaca ccgtttgact
     2941 gcgcatgggt tgatctcgcg aaagatatga tgcgtgtggt gacaggagac aaaatgtcag
     3001 tgcttgccgg gggctctctg ccctccacga taaggtggca ttcaaaaatt ttttcattac
     3061 ctgaaagaac ctctttttcc tgtatcaggg tgaaatctcc ggcgcctgag cgggtgggga
     3121 tcaccattat ggctgatgat gttcctgtga ttcattttgc gccgggtacg tttaagggaa
     3181 gtgtggtgag acttccggca gcaaccgggc aaaactggca ggtgatggta tccggattcg
     3241 ggcaggtgga acgaataacc ctgagtacat cgatgtcgga gttaccgata tgagcaaaaa
     3301 accgtggcgg gcggggaagg atttatccac tgttgtggag aatatggaaa ttggcaccgg
     3361 gcagcgtggt gacggacgtc acgcgtttgt gacccgtgaa gaactggtag gtcttaaact
     3421 cgcccggcgt cgaacatcgg gtggtgcctc atatgcactg aatccgggta ttgagattga
     3481 cagtacttta atgactgttg attttcccac aaaaccgctg aattttaagg cgacaggagg
     3541 atttggctcg gttcttcttg aatgggatat gcctaattat cgcggacatt cactgactga
     3601 aatctggcgg ggtacggagg atgaccttgc tgatgcagtg ctggttgcca cgacgccggg
     3661 gcaggtttac ggcgatccgg ttgaccctgg ctggtcggga ttttactgga tacgttttgt
     3721 taacgcggca ggagtgaaag gtccatggaa tgctgaaaaa ggcactcagg cacaaacaca
     3781 gatcggcgtg aaggccatca ttgaccagat ccgcgatgag gctgcaaagt cgccggttgt
     3841 gtccgagctg cgtaaagaaa taaaaaacgc gcaggggcag gctgtaaagg atgctgcaat
     3901 taagacaacc gaagttgtgg ggactctcag ggaagaaacg acaagaacga ttggtggtat
     3961 tgaaacccgc attagcacac tggattcgtc aaccagtgaa tcgcttaatg aggtcgacaa
     4021 gcgcatcact aaattggata aagaaggcgg tgaagctttt cttgcaatgt ggtcaaaaaa
     4081 agcgggagtt gatggtatca ctgcggggat cgggattgtc gccggaaaag acagtgaagg
     4141 caggcctgta agtcaggttg caatttctgc gtcgcagttg tttgtctttg acccgaacaa
     4201 cccggataac accgcctatc cgtttgcggt atcaggtggc aaggttgtga tcccgaaagc
     4261 gatgatttat gacgcggtga ttgaaacact ggtgtcgcgg aaggttgtgg cggatgaggt
     4321 aaaagccggg gtaagtatca cttcgccagt tatccggagt gccgttattc agaacggaaa
     4381 ctttcaggtt gattctcagg gtaacctgaa tattggaggc cttttcagtg ttacgtcaca
     4441 agggcaactg acaattcgtt actctaatca gaatgtagga ctggtgatcc gcaatgataa
     4501 aattgaggtt tatgatcaga atggacgact ggctgttcgc ataggcagat tacgctgatc
     4561 aggaggtgag tattggaata cggttttgcc atttataaca gaaataacgt taatgttacg
     4621 ggcgtgttga ctccggtatt tttcctggac agatttacag cggagtctgg ctcaaagacg
     4681 tacacgaata aacccgacgg gaaatcattg caggctgtat gttgtttatt tccctggaat
     4741 aatgtatttg cggatcggaa agtaccgaag ataaccatta atggcaatac ggtgacgtgg
     4801 tcgaatcttg agcagggtat gggatcttat atttatacat tctggggata agtgtcatgt
     4861 atggtttgag cattatgaag ccggatggca gcgtatggat aagtccaggt tttacgccgc
     4921 agtgtctgat caacaaaggc accataccgg cgactgaaaa gtcttttttt aaaacatcaa
     4981 tcccgtcagg caaaagttgt tttttcttta tcagaacaga gaagaaggcc gatgtcatgt
     5041 acacgcatga acagattgat ggatatcatg cactaaggct tcatgtaatt gtcaggggaa
     5101 cgaaccctgg tgttacgacg gtttatgctt tcgcgaatat ggttactcca ccttctgagt
     5161 atggtatcgc catgtataac ccggacggtg agatgattta tcatggcgaa atgatgctgc
     5221 ttgacgcgaa gttaatacct gttgatatca aatttgaaaa ggaccttgga tatccatgcg
     5281 caatcatgcc tgcactggtc gggtattata actggaaaag aactccttat gatcgaccga
     5341 tttataccac atccactggt gctacaggaa ataaaatata ttcctgtgag cattattccg
     5401 gtggtgcaac atgggatatt cgaaagccgt atatagataa ggtcctggtt attaatacat
     5461 cagtatatga ttagttgaag cgagtcttta atattcattt aaaatgtcta aaaagatgta
     5521 ttattaaaaa gtttagcgtg ttatctgaat acaggatatc ttaaatgaag agtatagcaa
     5581 cactggttgt gtgtgcaatc tccgggattg cctgtgtaaa tttatctgca catgcagcag
     5641 aaggaaacca tacaatttct ctggggtatg cgcactttca gtttccggga ctgaaggatt
     5701 ttgtaaagga tgcgactgct cataacaggg agactttcag tcatttcgtc aacagaaact
     5761 acttttcttc attgggcgaa tatacagatg gtcgggtcag tggatatgaa ggcaaggata
     5821 aaaatccaca gggcattaat atcaggtatc gctacgagat aacggatgat tttggcgtta
     5881 tcacctcttt tacatggacg cgttctctca ctaactcaca gacatttatt gatgtgcagt
     5941 cagccgatca tagcaggaag attaagaatc cggcagcttc tgccagaacg gatatcaggg
     6001 cgaattactg gagtctgtta gcggggcctt catggcgggt taatcagtac atgagtttat
     6061 atgcgatggc agggatgggc gttgctaaag ttagcgctga cctgaaaatt aaggacaata
     6121 ttaacagtag tggcggattt tctgaaagca acagcacgaa aaaaacctcc cttgcgtggg
     6181 ctgcaggtgc acagtttaac ctgaatgaga gtgttacact ggatgtggct tacgaaggtt
     6241 ccggctctgg cgactggcgc acgagtggcg ttactgctgg cattggcctg aaattctgac
     6301 ctgtatccgg taaccgttta ctacccgctg tgatggcggg ttttttattg cccgtacagg
     6361 gcaaaaaccg taaattatgc gtgggtgcct ttcggctgat ggctggaggg agaacctgaa
     6421 ggcctgatgt ggaaaggccc cgagtcaact taacgttaac ccgaggccct aacacttcgt
     6481 accttgacaa gtagaaggtt agcgcctctc cactcaggag gcaagcgcta tgtcgcaaaa
     6541 atcgcttatc accgtcacaa tttgtatgac ggttatcttc accatctgga tgttacacgg
     6601 ctcgttgtgt gagttccggc tgaatttgtg gggagcggag tttgcggcgt tcttacagtg
     6661 taagcagtag gaaaaccgcg gcggggacga aagtccccgt caactggttg ctgaggttca
     6721 gccgatatgg cacccgtttc aggtgagaga atgaacgata aaattctccg gtatatgcag
     6781 cgtgtggtga gaaattcccg caaccctgaa tttatgaatg aagttaaaga cgcctgcctt
     6841 aaaaagcagg cgttttgttt tgaggcacct gatggctttc tggtgctgcg ttctgtgctc
     6901 agtgctgatg gtatccctta tgttctggtg ttgctgggcg tgtgtacggg gagtaacagc
     6961 gttgagcgtt atctgccgga ggtgaagaca ttaacccgtc tggctggcgg acgctgggct
     7021 gaatttcata cggcaaggcg gggatttatc cggctgggaa aacgtctggg ctttgagcga
     7081 atgccggatg atgaggatgg cttcatggtg ttcaggatag cggtctgact gctatagttt
     7141 tcatcattgt gtttaaacca acattgtaat tcacattctg accctgctcc ggcagggttt
     7201 tttgttatcc agggggccat tatgggtgga agtaaaggcg gtggtgatac caaagtaaaa
     7261 ccaacagcag cgcaaatagc gcaggaagaa gtggcctgga aagggtggca ggattatcaa
     7321 aaaatcctcc gtccggcaga agataacttc atggaaaagg tcgatgacct taacagtgag
     7381 cagcagtacg aaaatatcgc tggcacaaca aatcttggtt atcagaaaca gtttggtgaa
     7441 gcgcgaaggg agctggcggg taatcttgct cagtccggcg ttgacccatc cagtggtcgt
     7501 tttaatgcgg taatggatgc aaatcaaagc gaccaggtga ccgggcagat tgacacaacc
     7561 acacgggggc aggtgtcgca ggcagataag tatgttgccg ggctacagga tgttgctgct
     7621 ctcggttctg gtcagaaggc ggatgcgtta cagagtttta actctctggc agacagcagt
     7681 ctggcaaaag ctaaatcgga tgcacaggcg gcgtttacga aacagcaggg gcgagcctct
     7741 cttgttggcg ctggtctggg tgcggcaggt gcgtatgcga tgcataaggc tggcggtagc
     7801 ggaggcggtg ctaaaacacc tggcaccggc gctaatgcca ttcagcatca ggctcagaac
     7861 tggagactgt gattatggag ta
//
LOCUS       DAZKFU020000217         7860 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZKFU020000217 DAZKFU020000000
VERSION     DAZKFU020000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.219"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(390..1583)
                     /gene="tyrB"
                     /locus_tag="ACHOQQ_RS19190"
                     /old_locus_tag="pgaptmp_003838"
     CDS             complement(390..1583)
                     /gene="tyrB"
                     /locus_tag="ACHOQQ_RS19190"
                     /old_locus_tag="pgaptmp_003838"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGU4472042.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNALPHGASLYLPMEGLNSYRHAIAPLLFGADHSVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1836..2915)
                     /gene="alr"
                     /locus_tag="ACHOQQ_RS19195"
                     /old_locus_tag="pgaptmp_003839"
     CDS             complement(1836..2915)
                     /gene="alr"
                     /locus_tag="ACHOQQ_RS19195"
                     /old_locus_tag="pgaptmp_003839"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGU4472043.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2968..4383)
                     /gene="dnaB"
                     /locus_tag="ACHOQQ_RS19200"
                     /old_locus_tag="pgaptmp_003840"
     CDS             complement(2968..4383)
                     /gene="dnaB"
                     /locus_tag="ACHOQQ_RS19200"
                     /old_locus_tag="pgaptmp_003840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4472044.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4466..5449
                     /locus_tag="ACHOQQ_RS19205"
                     /old_locus_tag="pgaptmp_003841"
     CDS             4466..5449
                     /locus_tag="ACHOQQ_RS19205"
                     /old_locus_tag="pgaptmp_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGU4472045.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5615..5857)
                     /gene="pspG"
                     /locus_tag="ACHOQQ_RS19210"
                     /old_locus_tag="pgaptmp_003842"
     CDS             complement(5615..5857)
                     /gene="pspG"
                     /locus_tag="ACHOQQ_RS19210"
                     /old_locus_tag="pgaptmp_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGU4472046.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5991..7028)
                     /gene="dusA"
                     /locus_tag="ACHOQQ_RS19215"
                     /old_locus_tag="pgaptmp_003843"
     CDS             complement(5991..7028)
                     /gene="dusA"
                     /locus_tag="ACHOQQ_RS19215"
                     /old_locus_tag="pgaptmp_003843"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGU4472047.1"
                     /translation="MYGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7391..>7860)
                     /locus_tag="ACHOQQ_RS19220"
                     /old_locus_tag="pgaptmp_003844"
     CDS             complement(7391..>7860)
                     /locus_tag="ACHOQQ_RS19220"
                     /old_locus_tag="pgaptmp_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HGU4472048.1"
                     /translation="HLQAIGSGFLTFVNACQKYDDNSLKLFASLLISLSYSSAYTDLA
                     GAEKVNINDDNENYLTAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNE
                     GKLAPDILSKMRDAAPKLIDFAKSFDPNSKEKIKIYTGTSKLIYDLFGVKSEK"
BASE COUNT         2055 a         2040 c         1863 g         1902 t
ORIGIN      
        1 taatcaatat aaggttaaac ttacacaaag cggaaaaaaa gagttctctt taactttaga
       61 atttaacgat actataattg aatctcagaa agtcacaggc aataaggcaa agcatattct
      121 tgaggatata gaaaaattct atcgtaacaa acccgatact tattattaaa tttaataatg
      181 gttagatgtg cagaaattat tactccacac aaaaaaacag gataaggaat tccagataat
      241 tgggataagc ttaacaataa aagaactaac aacgtttatt atctcttacg tagaacgatg
      301 gcatcaaaaa atcatctata aagaaagagt gaaagcgacg acagtttaat ttcactgcag
      361 gctgggtaac tccagcctgc tttcctgcat tacatcaccg cagcaaacgc ctttgccaca
      421 cgttgtacat ttgccgtatt taacccggcg acacacatgc gaccgctggc gatgagatag
      481 acaccaaatt cttcacgtag tcggtcaacc tgagcggcac ttaaaccggt ataactgaac
      541 atgccgcgct gattaagcag ataatcgaaa ttgcgttctg gcatctctgt gctcaatacc
      601 ttcaccagtt cctgacgcat tgccagaatg cgagtacgca tctcttctac ttccgccagc
      661 cagctggctt tcaatgcctc gtcattcagc accgcagcca ccacctgcgc accaaaattc
      721 ggcgggctgg agtagttgcg gcgaactgtt gctttcaatt gccccagtac gcggcctgca
      781 gcttcggcat cttcacacag aacagaaagt ccgccgacgc gctcgccgta aagggagaaa
      841 attttcgaga acgaattgct caccagagcg ggtaatccag cgctggcaat ggcgcgaatg
      901 gcgtaggcat cctcttccat accggcacca aatccttgat aggcaatatc aaggaatggg
      961 ataagctcgc gggctttgag aatttcaatc accgcatccc actggtcatt agtgagatcg
     1021 gcacccgttg ggttgtggca acatggatgc agcaacacaa tactgcgggc aggtaatgtt
     1081 ttcagcatcg ccaacaggtc attaaagcgc acgccgttag tcgcttcgtc ataccagggg
     1141 taagtactta cttcgaatcc ggccccggcg aatattgcta cgtggttttc ccaggtagga
     1201 tcgctgaccc agacgcctga ttccggaaag tagcgtttca ggaaatccgc gcccactttc
     1261 aatgcccctg agccgccaag ggtttgaatg gttgctacgc gctgttgttg cagtaccgaa
     1321 tggtcggcac caaacagcag cggcgcaatg gcatggcgat agctgttaag cccttccatc
     1381 ggtaaataaa gcgaagcgcc atgaggcagc gcattcaggc gcgcttctgc atcagccacg
     1441 gctttcaatt gtggaataat tccgtcttcg ttgtagtaca gaccgatact taaattcact
     1501 ttgtcgctgc gagggtcttc tttaaaacgc tccataagcg taagaatcgg gtcgccagcg
     1561 taggcgtcaa ctttttgaaa cacgcgatgg ttctccaggt ttacgggcag gtggttaaaa
     1621 cacaataaac cggaagaagg cgaagatcga gtggatgttc aggggcgacc ggcaattagc
     1681 aacagagtga gactcatgac aaacgtacat ccgccagagg cacgaccttt ataagagcat
     1741 gaaagaatat caacttattg aattggtagg attttagtgg ccggataagg cattcacgcc
     1801 gcatccggca cagacaatca aatattacag aacgattaat ccacgtattt catcgcgacc
     1861 cttgaagtca ggcgcgtaat aagttcgtaa gcgcttactt tcgtcatttc agcgatacgt
     1921 tctacgggca aaccttcacc ccacaaaatg accgggtccc cggctttatc ctgtgcctgt
     1981 ggacctaagt ctacgcagat catatccatc gcgactcgcc cgacaatcgg cacttcgcga
     2041 ccgttcacca gcactggcgt accagacggc gcggcgcgcg gataaccatc gccatagccc
     2101 atcgcaacta caccaagacg agtatcacgt tcgcttatcc aggttccacc ataaccgaca
     2161 ggctctccgg ctttatgctc acgcacggca atcaggctgg aggttagtga catcactggc
     2221 tgacagccaa aatcggcacc ggtggagcga tcttccagcg gcgagacgcc ataaagaatg
     2281 atgcccgggc gcacccagtc aaaatgcgac tgtggccaca gcagaatgcc acccgacgcg
     2341 gcaatggaac gttgacctgg tttgccttcg caaaaggtat taaagatagc gagttgtttc
     2401 tcggttgcgc cacattttgg ttcatccgcg cgcgcaaaat ggctgacgat attcaccggc
     2461 tgacgaacgt ttttgcactg ggtcaggcga tgataaaacg cctcagcctg ttccggcctt
     2521 acgcccagac ggtgcatacc ggtatcgagt ttcatccaga cggtaaccgg ctcgtccagg
     2581 ctagcctctt ccagcgcagc cagctgttct tcgttatgca cggcggtatg aaaatgttgc
     2641 gcagaaatcg tcggtagatc tctggcatca aaaaagcctt cgagtaacag tacaggtttg
     2701 gtgattcccc ccgcacgcag tcgcagagct tcttcgagac gggctacgcc aaaggcgtca
     2761 gcatcgggga gcgttcgcgc ggtctcaaga agaccgtgac cataagcgtt cgctttcacc
     2821 accgcaacca ttttactggc aggagccagt tcacgaagac gttgcaggtt gtgtcgcaga
     2881 gcgcggcggt taatcacaac agttgccgct tgcatttgtg ttccttgata agtgtttgct
     2941 ttaattacct aattcataaa ataattatta ttcgtcgtcg tactgcggcc ccgcatagtt
     3001 gtcgaagcgc gaccattgac cgttaaaggt caggcgtacc gtcccgattg ggccgttacg
     3061 ttgtttaccg ataataattt ccgcgatgcc ttttaaatca ctgttttcgt gatacacctc
     3121 atcacgatag ataaacatga tcaagtccgc atcctgctcg atagagccag attcacgcag
     3181 gtcggagttg accgggcgtt tgtcggcacg ttgttccaga gaacggttca actgggacag
     3241 cgccaccacc ggcacgttca gttctttcgc cagtgctttc agcgagcggg agatttctgc
     3301 aatttccagc gtacggttat cggaaagcgc cggtacgcgc atcagttgca ggtagtcgat
     3361 catgataagc ccgatgccgc cgtgttcacg ggcaatacgg cgtgcgcggg aacgcacttc
     3421 cgttggcgtc aagccggagg agtcatcgat atagatattg cgtttttcga gcaaaatacc
     3481 catggtgccg gaaatgcgtg cccaatcttc atcatcgagc tgcccggtac ggattttagt
     3541 ctggtcaacg cgcgacagcg acgccagaga acgcatcata atttgttctg atggcatctc
     3601 cagcgagaag ataagtaccg gtttatcctg caacatcgcc gcgttttcga cgaggttcat
     3661 cgcaaatgtc gttttaccca tcgacggacg cgcggcgacg atgatcaaat ccgacggctg
     3721 caagccagcg gtttttttgt tgagatcgtc ataaccggtg tttaccccgg taacgccatc
     3781 gtgtggctgc tgaaacaact gctcaatacg cgccacggtg gcgtcgagca catcggcgat
     3841 gttcttcggc ccttcgtctt tgttcgcacg actttcggca attttaaaga cgcgggattc
     3901 agccaggtcc agcagatctt cgctggtacg cccctgcgga tcaaaaccgg cttcggcaat
     3961 ctcattcgca accgagatca tctcacggac aacggcacgt tcacgcacga tgtccgcata
     4021 ggcactgatg ttcgccgcac ttggcgtatt ttttgacagc tctgccagat aagcaaaacc
     4081 accgacgcta tcgagttgcc cctggcgttc cagcgattcc gcaagggtaa tcagatcgat
     4141 aggactaccg ctttcctgca aacgcgccat ttcagtaaag atatgacggt gtgggcgggt
     4201 gtaaaaatcg tctgctacca cacgctcggc tacatcatcc cagcgttcgt tatccagcat
     4261 taaaccgccc aacaccgact gctccgcttc gatcgagtgc ggaggcactt tcagcccggc
     4321 aacttgtgga tcgcgttcgc ggggttcagc ctgctgtttg ttgaagggtt tatttcctgc
     4381 catagtgaat ggagttaccg agataaagaa tgggtcgaaa ctttaccata tgaagcagac
     4441 cctgacgata cgttctggag gacacatggc aacacgaatt gaatttcaca agcacggtgg
     4501 cccggaagta cttcaagccg tagagttcac tcctgccgat ccggcggaga atgaaatcca
     4561 ggtcgaaaat aaagccatcg gcatcaattt tatcgacaca tatatccgca gcggccttta
     4621 cccgccgcca tcgctaccca gcggattagg caccgaagca gcaggcatcg tgagtaaagt
     4681 cggcagtggt gtaaagcata ttaaggcagg cgatcgtgta gtctatgcgc agtcggcgtt
     4741 aggcgcttac agctctgtgc ataacattat tgcggataaa gcggcgattc tgcctgcggc
     4801 aatttctttt gagcaagctg cggcatcctt cctgaaaggc ttaacggttt attatctgct
     4861 gcgcaaaacc tatgaaatta aacccgatga acagttcctg ttccacgcag cggctggcgg
     4921 cgttggcttg attgcctgcc agtgggcaaa agccctgggc gcgaaactta tcggcaccgt
     4981 aggaaccgcg caaaaagcgc agagcgcgct aaaagcgggc gcgtggcagg ttattaacta
     5041 tcgtgaagag aatctggtcg agcggttaaa agagatcacc ggtggtaaaa aagtgcgcgt
     5101 ggtgtacgat tccgtgggca gagacacctg ggaacggtcg ctggattgcc tgcaacgccg
     5161 cggcttaatg gtgagttttg gcaactcatc aggtgcggtt accggtgtga acttaggcat
     5221 tctcaatcag aaaggctcgt tgtatgtgac acgcccttcc ctgcaaggct atatcaccac
     5281 gcgggaggaa ttaaccgagg ccagtaatga actgttctct ttgattgcca gcggtgtgat
     5341 taaggtcgat gtcgccgagc agcagaaata tccgctgaag gatgcgcagc gtgcgcatga
     5401 gattctggaa agccgggcga cgcaaggttc cagcctgttg attccataaa agaaataggg
     5461 cttccacctg ggaagccctt tctttttata gttcggctgt atgtagggta cagcacgatg
     5521 aatctgttag aggcgcaata gtgacagatt tgattatcaa ttcctatttt gttctaagga
     5581 taaaacctta ggttgtgatc atccgcacaa tcccttagta acgccagcgg tcataacgct
     5641 gatatttcgg cacttttggt gctttaatcg ccttaataac ccacaccacc gcaatcgcca
     5701 ggagtaacca cggcagcaac ttaatcatca atgccagcat accgccgagg aacataatgg
     5761 ccgtcgccac aaccagcgcg gcgataatgc ccagcaacga aacgccggtg accatcaaca
     5821 tgacaaaaaa gccaatcaca aaaagtagtt ccagcatgat gctctcccaa atatgaaatc
     5881 tcttgctggc attacaagaa tcatgccaaa aataatctat tgatttaaca gcaaaacgcc
     5941 ccgcgaattt gcgcagggcg tggtgaattt gactactttt tggtgaaaag ttaacgctta
     6001 tccgccacca gtttgagcgc gtgttccagc acgttaatgt cagcacccgc tttatgggca
     6061 ttttcactta aataacgccg ccactgccgc gcgccaggaa taccctggaa caagcccagc
     6121 atatgccggg taatatggcc gagatacgtc ccctggctga gttcacgctc aatgtacgga
     6181 tacatggcgc gcactaccgc caccggatcg gcatcggtat ccgaggaacc aaagatctct
     6241 cggtctaccg ccgccagaat acccggattc tgatacgcct cgcgcccgac catcacgcca
     6301 tccatatgtt gcaaatgcgc tttagcctct tccagcgact tgataccacc gttaatcgac
     6361 atcgtcagat gcggaaagtc acgcttcagt tgatacacac gcggataatc gagcggcggg
     6421 atttcacggt tttctttcgg gcttaacccc gaaagccagg ctttacgtgc gtggatgatg
     6481 aacatctcac actcgccttt gccggaaacg gtgttgatga aatcgcagag aaattcatag
     6541 ctgtcctggt catcgatgcc aatacgcgtt ttcaccgtca ccggaatcga caccacatcg
     6601 cgcatcgctt tcacgcagtc ggcaaccagc tgcgcattac ccatcagaca cgcaccaaac
     6661 atgccgttct gcacccggtc agacgggcag ccgacattca ggttgatctc atcatatcca
     6721 cgcgcttctg ccagcttcgc acactgcgcc agcgccgccg gatcgctacc gccgagttgc
     6781 aacgctaccg gatgttcttc ttcactgtac gccaggtaat cacctttacc gtgaataatc
     6841 gcccctgtag tcaccatttc ggtatacagc aacgtattgc gggaaagcag acgcaagaaa
     6901 tagcggcaat gtctgtccgt ccagtcgagc attggtgcaa cgctaaaccg accactccag
     6961 tgaacgtcag ttttttcagg cattgcgctg gtttggttga ttttttgcat ttcagaatta
     7021 ccgtacattt tcaaatgtag aaattatttt atcgatatat catggggtta tgttattcag
     7081 catcactgtt caggaggctc aatagcggga tactatacca taacaacagg aagcgcctgt
     7141 ctcattgcaa aagaaaattg agatcctctc aaggcatgaa gctctcacga agtgatggaa
     7201 ataatcttat tagccgttag ctttgttaag gccaatgata aacaatccag gttcgacgat
     7261 aaataaaaat cacacattaa actccggtga tatctctccc tgctaatagc actgatagag
     7321 aaaaaaagaa cccaataagc attgggttct tttatgtaat gccttccata ccatcgaaga
     7381 acttcacata ttatttctcc gatttaaccc cgaataaatc ataaattaat ttagaagtgc
     7441 ctgtatatat tttaatcttt tcctttgagt ttgggtcaaa cgacttagca aagtcaatta
     7501 atttcggtgc agcatctcgc attttgctta aaatatcagg ggcgagcttt ccttcattca
     7561 ccagatgtga catcttatcc agatagccat caaaattcat ttctccccca tctgccagac
     7621 gcttcatctc tcccaggtac ttcttcatat cacgttgaga taactcttca aactgagctg
     7681 tcagatagtt ctcattatca tcattaatat tcactttttc tgctcctgct aaatctgtgt
     7741 aggcactaga gtatgaaagt gaaatcaaca gcgaagcgaa taattttaag ctgttgtcat
     7801 catatttctg acatgcatta acaaacgtta aaaaaccaga acctattgct tgtaaatgta
//
LOCUS       DAZKFU020000218         7796 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZKFU020000218 DAZKFU020000000
VERSION     DAZKFU020000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.220"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..721
                     /gene="yneK"
                     /locus_tag="ACHOQQ_RS19225"
                     /old_locus_tag="pgaptmp_003845"
     CDS             <1..721
                     /gene="yneK"
                     /locus_tag="ACHOQQ_RS19225"
                     /old_locus_tag="pgaptmp_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGU4472049.1"
                     /translation="KPVTMGRVFQTGVERVLFLFLNDFIEQFPMINPGVPIKRAHTPH
                     IEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDPNLITAI
                     LDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDLFLFFASMLL
                     HYHQFMNPNESDLNNVLMPASLSDDKIIKHMARKTLKLFVKNETPPKVTHEDLVKNRP
                     RSPVRPPIPATSKTPDIPDRH"
     gene            872..2062
                     /gene="ydeA"
                     /locus_tag="ACHOQQ_RS19230"
                     /old_locus_tag="pgaptmp_003846"
     CDS             872..2062
                     /gene="ydeA"
                     /locus_tag="ACHOQQ_RS19230"
                     /old_locus_tag="pgaptmp_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGU4472050.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2087..2752)
                     /gene="marC"
                     /locus_tag="ACHOQQ_RS19235"
                     /old_locus_tag="pgaptmp_003847"
     CDS             complement(2087..2752)
                     /gene="marC"
                     /locus_tag="ACHOQQ_RS19235"
                     /old_locus_tag="pgaptmp_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGU4472051.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2964..3398
                     /gene="marR"
                     /locus_tag="ACHOQQ_RS19240"
                     /old_locus_tag="pgaptmp_003848"
     CDS             2964..3398
                     /gene="marR"
                     /locus_tag="ACHOQQ_RS19240"
                     /old_locus_tag="pgaptmp_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGU4472052.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3419..3802
                     /gene="marA"
                     /locus_tag="ACHOQQ_RS19245"
                     /old_locus_tag="pgaptmp_003849"
     CDS             3419..3802
                     /gene="marA"
                     /locus_tag="ACHOQQ_RS19245"
                     /old_locus_tag="pgaptmp_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGU4472053.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3834..4052
                     /gene="marB"
                     /locus_tag="ACHOQQ_RS19250"
                     /old_locus_tag="pgaptmp_003850"
     CDS             3834..4052
                     /gene="marB"
                     /locus_tag="ACHOQQ_RS19250"
                     /old_locus_tag="pgaptmp_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGU4472054.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(4083..4982)
                     /gene="eamA"
                     /locus_tag="ACHOQQ_RS19255"
                     /old_locus_tag="pgaptmp_003851"
     CDS             complement(4083..4982)
                     /gene="eamA"
                     /locus_tag="ACHOQQ_RS19255"
                     /old_locus_tag="pgaptmp_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGU4472055.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGALLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            5177..6364
                     /gene="ydeE"
                     /locus_tag="ACHOQQ_RS19260"
                     /old_locus_tag="pgaptmp_003852"
     CDS             5177..6364
                     /gene="ydeE"
                     /locus_tag="ACHOQQ_RS19260"
                     /old_locus_tag="pgaptmp_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGU4472056.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVDSVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGLVLTNLPPSSLFIILALVIIAAWVLMLKGIRARPWRQP
                     ALC"
     gene            6491..6586
                     /gene="mgtS"
                     /locus_tag="ACHOQQ_RS19265"
                     /old_locus_tag="pgaptmp_003853"
     CDS             6491..6586
                     /gene="mgtS"
                     /locus_tag="ACHOQQ_RS19265"
                     /old_locus_tag="pgaptmp_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGU4472057.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            6586..6691
                     /gene="mgtT"
                     /locus_tag="ACHOQQ_RS19270"
                     /old_locus_tag="pgaptmp_003854"
                     /pseudo
     CDS             6586..6691
                     /gene="mgtT"
                     /locus_tag="ACHOQQ_RS19270"
                     /old_locus_tag="pgaptmp_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
     gene            complement(6806..7696)
                     /gene="dgcZ"
                     /locus_tag="ACHOQQ_RS19275"
                     /old_locus_tag="pgaptmp_003855"
     CDS             complement(6806..7696)
                     /gene="dgcZ"
                     /locus_tag="ACHOQQ_RS19275"
                     /old_locus_tag="pgaptmp_003855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU4472058.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FAAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         2035 a         1921 c         1759 g         2081 t
ORIGIN      
        1 aaagcctgtc actatggggc gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt
       61 tctgaatgat tttattgaac aatttccaat gataaaccct ggcgttccca tcaaaagagc
      121 gcatacacca catattgaac ccctgcctcc ggatcaccat accgctgcag attacttacg
      181 ccagtttgat ttgcttgtcc tgaattttat ctctcgcggt aattttgtta tactcccccg
      241 attatggaat aactctgagg ttcacagatg gttcgtcaat aaagatccta acttgatcac
      301 cgccattctc gacataacgg acagcgaatt aaaagaggat ttattgcaaa gcctgatgga
      361 ttcattaggt tctaacaaac atgtactacc cgaagtctgc atctgctttt tatccctttt
      421 agcagaacaa gaatctcctc attttcagga cttgttttta tttttcgcca gtatgttatt
      481 gcactatcac caatttatga atcccaatga aagtgatttg aataacgtgt tgatgccagc
      541 atcattaagt gatgataaaa ttatcaaaca tatggcgcgc aagaccctca aactttttgt
      601 aaaaaatgaa acaccgccaa aagttactca tgaagacctg gtgaaaaaca ggcctcgctc
      661 ccctgtcaga ccacctatac ccgcaacctc caaaacgcca gacattcctg atcgtcatta
      721 aaccgtgatg tttaccgtct ctctgacgcg tgaaagaatc agcgtcagag aaacggaaaa
      781 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
      841 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
      901 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
      961 tgtccctgtt ggcctactct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     1021 cggcattatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgct
     1081 aatgaccagc caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     1141 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     1201 tgtggctttt gcacatgcga ttttctggtc aattacggcg tccctggcga tccgtatggc
     1261 tccggccggg aagcgagcac aggcattgag tttaattgcc acaggtacag cactggcaat
     1321 ggtattagga ttacctctcg ggcgcattgt ggggcagtat ttcggttggc gaatgacctt
     1381 cttcgcgatt ggtatcgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     1441 actgcccagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     1501 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     1561 ctatattgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     1621 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggga aactgggtaa
     1681 tcagtatgcg tctgcgttgg tgagtacggc gattgcgctg ttgctggtgt gtctggcact
     1741 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     1801 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     1861 caccgacgtc gcgatggcgc tattctccgg gatatttaat attggaatcg gggcgggtgc
     1921 gttggtaggt aatcaggtga gtctgcactg gtcaatgtcg atgattggtt atgtgggcgc
     1981 ggtgcctgct tttgccgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     2041 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     2101 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     2161 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     2221 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     2281 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     2341 tcatcgcaat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaagcaatat
     2401 tggcgctggg ttcatcttcc agctcttccg acttgctttt cgcttccggt gaatcaatcg
     2461 ctttctgttg cggaaagagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     2521 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgcccg gcgtaatacg
     2581 ccaccatcat gatggcaaag acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     2641 cactgttcat gttgcctgcc aggccaagaa ataacgcgac agttgttaat gggttagcta
     2701 acggcagtaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     2761 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     2821 cactctttag ctaaccttgc atcgcattgg ccaaaacttg aaccgattta gcaaaacgtg
     2881 gcatcggtca attcattcat ttgacttata cttgcctggg caatattatc ctctgcaact
     2941 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     3001 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aatgagtatc
     3061 tgtctccgct ggatattact gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     3121 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     3181 gtatgctgga tcgcctggtc tgtaaaggct gggtggaaag gttgccgaac ccgaatgata
     3241 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgtgaa caatgccatc
     3301 aattagttgg ccaggacctg caccaagaat taacaaaaaa cctgacggcg gacgaagtgg
     3361 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaaagag gtatgacgat
     3421 gtccagacgc aatactgacg ctattaccat tcatagcatt ttggactgga tcgaggacaa
     3481 cctggaatcg ccactgtcac tggagaaagt gtcagagcgt tcgggttact ccaaatggca
     3541 cctgcaacgg atgtttaaaa aagaaaccgg tcattcatta ggccaataca tccgcagccg
     3601 taagatgacg gaaatcgcgc aaaagctgaa ggaaagtaac gagccgatac tctatctggc
     3661 agaacgatat ggcttcgagt cgcaacaaac tctgacccga accttcaaaa attactttga
     3721 tgtcccgccg cataaatacc ggatgaccaa tatgcagggc gaatcgcgct ttttacatcc
     3781 attaaaccat tacaacagct agttgaaaac gtgacaacgt cactgaggca attatgaaac
     3841 cgcttttatc cgcaatagca gctgcgctta ttctcttttc cgcgcagggc gttgcggaac
     3901 aaacccagca accgctcgtt acttcctgtg gcgatgtggt ggttgttccc ccatcgcagg
     3961 aacaaccacc gttcgattta aatcacatgg gtacaggcag tgacaaatcg gatgcgctgg
     4021 gcgtgcccta ttataaccaa caagccatgt agtatattca ggccccggca tatcggggct
     4081 tgttaacctc ttacctttac cgctttacgc caccgcaagc caaatacatt gatatacagc
     4141 ccggtcataa tgagcaccgc gccgaaaaat tgcagacccg ttaagcgttc atccaacaat
     4201 agtgccgcac ttgccagtcc gactacaggc accagtaacg ataacggtgc aacccgccag
     4261 gtttcatagc gtccaagtag tgctccccag atcccataac caacaattgt cgccacaaaa
     4321 gccagataca tcagagacaa gatagtggtc atatcgatag tgaccagact gtgaatcatg
     4381 gttgcggaac catcgagaat cagcgaggca acaaagaagg gaatgattgg gattaatgcg
     4441 ctccagatta ccagcgacat caccgccgga cgcgttgagt gcgacatgat ctttttattg
     4501 aagatgttgc cacacgccca actaaatgct gccgccaggg tcaacataaa gccgagcatc
     4561 gccacatgct gaccgttcag actatcttcg attaacacca gtacgccaaa aatcgctaag
     4621 gcgatccccg ccaattgttt gccatgcagt cgctcaccga aagtaaacgc gccaagcacg
     4681 atagtaaaaa acgcctgagc ctgtaacacc agcgaagcca gtccagcagg cataccaaag
     4741 ttaatggcgc agaagagaaa ggcaaactgt gcaaaactga tggttaatcc ataccccagc
     4801 agcaaattca gtggtacttt cggtcgtgcg acaaaaaaga tggccggaaa agcgaccagc
     4861 ataaagcgca aaccggccag catcagcggt ggcatgttat gaagccccac tttgatgacc
     4921 acaaaattta gcccccatac gaccactacc agtagcgcca acaccccatc ttttcgcgac
     4981 attctaccgc ctctgaattt catcttttgt aagcaatcaa cttagctgaa tttacttttc
     5041 tttaacagtt gattcgttag tcgccggtta cgacggcatt aatgcgcaaa taagtcgcta
     5101 tacttcggat ttttgccatg ctatttcttt acatctctaa aacaaaacat aacgaaacgc
     5161 actgccggac agacaaatga acttatccct acgacgctct accagcgccc ttcttgcctc
     5221 gtcgttgtta ttaaccatcg gacgcggcgc tacgctgcca tttatgacca tttacttaag
     5281 tcgtcagtac agcctgagtg tcgatctcat tggttatgcg atgacaattg cgctcactat
     5341 tggcgtcgtt tttagcctcg gttttggtat cctggcggat aagttcgaca agaaacgcta
     5401 tatgttactg gcaattacgg ccttcgccag cggttttatt gctattcccc tggtggacag
     5461 cgtcacgctg gtggtacttt ttttcgcact catcaactgc gcctattccg tttttgccac
     5521 agtgttgaaa gcctggtttg ccgacaatct ttcatccacc agcaaaacga agatcttctc
     5581 gatcaactac accatgctca atattggctg gaccgtcggc ccgccactcg gcacgctgtt
     5641 ggtcatgcag agcatcaatc tgcccttttg gctggccgct atctgctctg cgtttcccat
     5701 gtttttcatt caaatttggg taaagcgcag cgagaaaatc atcgccacgg aaacaggcag
     5761 tgtctggtcg ccgaaagttt tattacaaga taaggcactg ttgtggttta cctgctctgg
     5821 ctttctggct tcttttgtta gtggcgcatt tgcttcatgc atttcacaat atgtgatggt
     5881 gattgctgat ggtgattttg ccgaaaaggt ggtcgcggtt gttcttccgg tgaatgccgc
     5941 catggtagtt acgttgcaat attccgtggg ccgccgactt aacccggcta acatccgcgc
     6001 gctgatgaca gcaggcaccc tctgtttcgt catcggtctg gtcggtttta ttttttccgg
     6061 aaacagcctg ctattgtggg gtatgtcagc tgcggtattt actgtcggtg aaataattta
     6121 tgcgccgggc gagtatatgt tgattgacca tattgcgccg ccaggaatga aagccagcta
     6181 tttttccgcc cagtctttag gctggcttgg tgccgcaatt aacccattag tgagtggcct
     6241 agtgctaacc aacctgccgc cttcctcgct gtttatcatc ttagcgttgg tgattattgc
     6301 tgcgtgggta ctgatgttaa aagggattcg tgcaagaccg tggaggcagc ccgcgctttg
     6361 ttgatttaag tcgaacacaa taaaaattta attcagcctt cgtttaggtt acctctgcta
     6421 atatgtttct cattgagatg aaaattaagg taagcgagga aacacaccac accataaacg
     6481 gaggcaaata atgctgggta atatgaatgt ttttatggcc gtactgggaa taattttatt
     6541 ttctggtttt ctggccgcgt atttcagcca caaatgggat gactaatgaa cggagataat
     6601 ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt ttatgacggc
     6661 gaaaaaaaaa ccgccagtaa accggcggtg aatgcttgca tggatagatt tgtgttttgc
     6721 ttttacgcta acaggcattt tcctgcactg ataacggatc gttgacacag tagcatcagt
     6781 tttctcaatg aatgttaaac ggagcttaaa ctcggttaat cacattttgt tcgtcaataa
     6841 acatgcagcg atttcttccg gtttgcttac cctcatacat tgcccggtct gctcttccaa
     6901 tgaccacatc cagaggctct tcaggaaatg cgcgactgac acctgctgtc acggtaatgt
     6961 tgatatgccc ttcagaatgt gtgatggcat ggttatcgac taactggcaa attctgacac
     7021 ctgcacgaca tgcttcttca tcattagccg ctttgacaat aatgataaat tcttcgcccc
     7081 cgtagcgata aaccgtttcg taatcacgcg tccaactggc taagtaagtt gccagggtgc
     7141 gtaatactac atcgccgatt aaatgcccgt aggtatcatt aaccaattta aatcggtcaa
     7201 tatccaacaa cattaaataa agattcagag gctcagtgtt gcgtaactga tgatcaaagg
     7261 attcatcaag aacccgacgc cccggcaatc ccgtcaaaac atccatattg ctacggattg
     7321 tcagcaaata aattttgtaa tcggttaatg ccgcagtaaa agaaagtaac ccttcctgaa
     7381 aagcggcgaa atgcgcgtcc tgccagtgat tttcaacaat agccagcatt aattcccgac
     7441 cacagttatg catatgttga tgggcagaat ccattagccg aacgtaaggt aattcatcgt
     7501 tatcgagtgg ccccagatga tcaatccacc gaccaaactg gcacagtcca taagaatggt
     7561 tatccgttat ttctggctta ctggcatctc tcgcgaccac gctgtgaaac atactcacca
     7621 gccactggta gtgggcatcg atagccttat tgagatttaa caagatggca tcaatttccg
     7681 ttgtcttctt gatcattgcc actccttttt cacagttcct tgtgcgcgct attctaacga
     7741 gagaaaagca aaattacgtc aatattttca tagaaatccg aagttatgag tcatat
//
LOCUS       DAZKFU020000219         7686 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZKFU020000219 DAZKFU020000000
VERSION     DAZKFU020000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.221"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(100..363)
                     /gene="cedA"
                     /locus_tag="ACHOQQ_RS19280"
                     /old_locus_tag="pgaptmp_003856"
     CDS             complement(100..363)
                     /gene="cedA"
                     /locus_tag="ACHOQQ_RS19280"
                     /old_locus_tag="pgaptmp_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGU4472059.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            546..2807
                     /gene="katE"
                     /locus_tag="ACHOQQ_RS19285"
                     /old_locus_tag="pgaptmp_003857"
     CDS             546..2807
                     /gene="katE"
                     /locus_tag="ACHOQQ_RS19285"
                     /old_locus_tag="pgaptmp_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGU4472060.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAIILNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2854..3612)
                     /gene="chbG"
                     /locus_tag="ACHOQQ_RS19290"
                     /old_locus_tag="pgaptmp_003858"
     CDS             complement(2854..3612)
                     /gene="chbG"
                     /locus_tag="ACHOQQ_RS19290"
                     /old_locus_tag="pgaptmp_003858"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGU4472061.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGVVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3625..4977)
                     /gene="celF"
                     /locus_tag="ACHOQQ_RS19295"
                     /old_locus_tag="pgaptmp_003859"
                     /gene_synonym="chbF"
     CDS             complement(3625..4977)
                     /gene="celF"
                     /locus_tag="ACHOQQ_RS19295"
                     /old_locus_tag="pgaptmp_003859"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4472062.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5081..5923)
                     /gene="chbR"
                     /locus_tag="ACHOQQ_RS19300"
                     /old_locus_tag="pgaptmp_003860"
     CDS             complement(5081..5923)
                     /gene="chbR"
                     /locus_tag="ACHOQQ_RS19300"
                     /old_locus_tag="pgaptmp_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGU4472063.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5931..6281)
                     /gene="chbA"
                     /locus_tag="ACHOQQ_RS19305"
                     /old_locus_tag="pgaptmp_003861"
     CDS             complement(5931..6281)
                     /gene="chbA"
                     /locus_tag="ACHOQQ_RS19305"
                     /old_locus_tag="pgaptmp_003861"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGU4472064.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6332..>7686)
                     /gene="chbC"
                     /locus_tag="ACHOQQ_RS19310"
                     /old_locus_tag="pgaptmp_003862"
     CDS             complement(6332..>7686)
                     /gene="chbC"
                     /locus_tag="ACHOQQ_RS19310"
                     /old_locus_tag="pgaptmp_003862"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HGU4472065.1"
                     /translation="NVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFV
                     LINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGM
                     ALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEM
                     FTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMD
                     TISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYG
                     SVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPS
                     GIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPT
                     GLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF
                     "
BASE COUNT         2061 a         2014 c         1724 g         1887 t
ORIGIN      
        1 aaggcagctt tttgttacag gtggtttgaa tgaatgtagc aatgaaatac aaaatttcag
       61 gccatgtaac tcccggcaaa accgggaggt acgtaatcct tactcagtca cttccccttc
      121 ctggcggatc tgatttgccc aacgctgggc ggattcaggc acagtaaacg ccggtgagcg
      181 cagaaatgac tctcccatca gtacaaacgc aacatatttg ccacgcagca tccagacatc
      241 acgaaacgaa tccatcttta tcgcatgttc tggcggcgcg ggttccgtgc gtgggacata
      301 gctaataatc tggcggtttt gctggcggag cggtttcttc attactggct tcactaaacg
      361 catattaaaa atcagaaaaa ctgtagttta gccgatttag cccctgtacg tcccgctttg
      421 cgtgtatttc ataacaccgt ttccagaata gtctccgaag cggggtctgg ctggtggtct
      481 atagttagag agttttttga ccaaaacagc ggccctttca gtaataaatt aaggagacga
      541 gttcaatgtc gcaacaaaac gaaaagaacc cacatcagca ccagtcacca ctacacgatt
      601 ccagcgaagc gaaaccgggg atggactcac tggcacctga ggacggatct catcgtccag
      661 cggctgaacc aacaccgcca ggtgcacaac ctaccgcccc agggagcctg aaagcccctg
      721 atacgcgtaa cgaaaaactt aattctctgg aagacgtacg caaaggcagt gaaaattatg
      781 cgctgaccac taatcagggt gtgcgcatcg ccgacgatca aaactcactg cgtgccggta
      841 gccgtggtcc aacgctgctg gaagatttta ttctgcgcga gaaaatcacc cactttgacc
      901 atgagcgcat cccggaacgt attgttcatg cacgtggatc agcagctcac ggttatttcc
      961 agccatataa aagcttaagc gatatcacca aagcggattt cctctcagat ccgaacaaaa
     1021 tcaccccagt atttgttagg ttctctaccg ttcagggtgg tgctggctct gccgataccg
     1081 tgcgtgatat ccgtggcttt gccaccaaat tctataccga agagggtatt tttgaccttg
     1141 ttggcaataa cacgccaatc ttctttatcc aggatgcgca taaattcccc gattttgttc
     1201 atgcggtaaa accagaacca cactgggcaa ttccacaagg gcaaagcgcc cacgatactt
     1261 tctgggatta tgtttctctg caacctgaaa ctctgcacaa cgtgatgtgg gcgatgtcgg
     1321 atcgcggcat cccccgcagt taccgcacca tggaaggctt cggtattcac accttccgcc
     1381 tgattaatgc cgaagggaag gcaacgtttg tacgtttcca ctggaaacca ctggcaggta
     1441 aagcctcact cgtttgggat gaagcacaaa aactaaccgg acgtgacccg gacttccacc
     1501 gccgcgagtt gtgggaagcc attgaagcag gcgattttcc ggaatacgaa ctgggcttcc
     1561 agttgattcc tgaagaagat gaattcaagt tcgacttcga tcttctcgat ccaaccaaac
     1621 ttatcccgga agaactggtg cccgttcagc gtgtcggcaa aatggtgctc aatcgcaacc
     1681 cggataactt ctttgctgaa aacgaacagg cggctttcca tcctgggcat atcgtgccgg
     1741 gactggactt caccaacgat ccgctgttgc agggacgttt gttctcctat accgatacac
     1801 aaatcagtcg tcttggtggg ccgaatttcc atgagattcc gattaaccgt ccgacctgcc
     1861 cttaccacaa cttccagcgt gatggcatgc atcgtatggg gatcgacact aacccggcga
     1921 attacgaacc gaactcgatc aacgataact ggccgcgcga aacaccgccg ggaccgaaac
     1981 gcggcggttt tgaatcatat caggaacgcg tggaaggaaa taaagttcgc gagcgcagtc
     2041 catcttttgg cgaatattat tcccatccgc gtctgttctg gctaagtcag acgccattcg
     2101 agcagcgtca tattgtcgat ggtttcagtt ttgagttaag caaagtcgtt cgtccgtata
     2161 ttcgtgagcg cgttgttgac cagctggcgc atattgatct cactctggcc caggcggtgg
     2221 cgaaaaatct cggtatcgaa ctgactgacg accagctgaa tatcacccca cctccggacg
     2281 tcaacggtct gaaaaaggat ccatccttaa gtttgtacgc cattcctgac ggtgatgtga
     2341 aaggtcgcgt ggtagcgatt atacttaatg atgaagtgag atcggcagac cttctggcca
     2401 ttctcaaggc cctgaaggcc aaaggcgttc atgccaaact gctctactcc cgaatgggtg
     2461 aagtgactgc ggatgacggt acggtgttgc ctatagccgc tacctttgcc ggtgcgcctt
     2521 cgctgacggt cgatgcggtc attgtccctt gcggcaatat cgcggatatc gctgacaacg
     2581 gcgatgccaa ctactacctg atggaagcct acaaacacct taaaccgatt gcgctggcgg
     2641 gagacgcgcg caagtttaaa gcaacaatca aggtcgctga ccagggtgaa gaagggattg
     2701 tggaagctga cagcgccgac ggtagtttta tggatgaact gttaacgctg atggcagcac
     2761 accgcgtgtg gtcacgcatt cctaagattg acaaaattcc ggcgtaaata tggataaggt
     2821 cacgtcccct ggggagtgac cttaccacct tacttacaca tcaagataac tacccaaccg
     2881 ataaccacgc tcagcaattg catatttcaa cgacgctgat gtcagcactt ccagctccgt
     2941 taaccgtggg aaacagtaag cactctggcg aatcgtatta tcgacaaacg ccggatggca
     3001 catgacttcc agcgagcgtt ccccgcgatg gctggaatca tcaagcactt gcaggaacag
     3061 tgcctcgcta atctcttcac cataaaacgc gctactgaat ccgtgcgaac tgcgcagatt
     3121 agccggtaaa tcaccagcgt tagatagcgg ctgacggtcg atgcgcaacg caatcccctc
     3181 ttcagccgca aacctggcaa caatagggaa aatctgcggg aacatatgca catgatgatg
     3241 gctatcaaga tgcgtaggtt tgcgtccaaa tagctcaatg aaacgcaaat actgactggc
     3301 aagctcctga gtaatttctt ccagcggtaa agcatcttct tctgccaact gccagatcca
     3361 ttttcccagc acaccatcgc gggttaaccc cggcatagct gtcagtggct tgcccatagt
     3421 aaggacaaag tgcatcccta tggccagact cggttcatca cgactcaact gcaccgcatg
     3481 gtcaatagcc tgcccattca ccagcgccgt cgtcgatgtg acaaccccat tgcgacaagc
     3541 ctcgataatg ccgtagttct gacctttgct taagccaaaa tcatcggcat taacaatcag
     3601 taagcgttcc ataatcagcc tcggttaatg tgctttttta agctctgcga tgcagtcggc
     3661 aaaatttggc agccatttct cgtgcgccag aatcatctcg cgtgccagca gctcagcatc
     3721 gcgatcggaa tgcaccaacg gactaaggtt tagcgccagt aacacatcgt taaattctcc
     3781 gctaagtgcg gcgttgctgg cagcaatctc gaagccttta atggtgtgaa tcagccccat
     3841 cactttatca tcgaaatgcg taatgcgcgg atgtggcgtc gcgccatcgc gccccagcgt
     3901 acaggtcatt tctaccgccc agtctgccgg aatattatca atatgcccat gatgcgggat
     3961 attaacgtaa tgttctgctt gcttatcgtt gtagatagcg ttgatcactt cgcacgctgc
     4021 atcagagtaa taagccccac cgcgctgttc cagttctttc ggcttaactt tcaactccgg
     4081 gtttttatac agctcaaaaa gttgtttctc gactttctgt actacctgcg ctcgtgcgcc
     4141 gcctttgtag tattcgccca tttcaatagc cagcatctct ttttgcttga agtaataaag
     4201 caaataagaa cacggcaaca gattcagaga acgaattaag ccttcgctaa atggcagatc
     4261 gaaaatattt ttaacgccag atgcttttaa ctgcccggat gccacaccat caagtaattc
     4321 ggcaaagcga gatttgccat ttaccagcac atccttaatg aacaccatat ggttgaggcc
     4381 gaacagatcg atagataaat catcgctgtc tttcagcatc agaacatcgc gaataaacat
     4441 cttcatgccg atcggaatat tacacacgcc gataaagcgt ttaaatccgg tatgacgata
     4501 aacggcttca gtgaccattc ccgccgggtt agtgaagtta atcacccatg cattcggaca
     4561 aagttcttcg acatctttta cgatgtcaaa aatcaccgga atggtacgca gacctttaaa
     4621 cagaccgccc gcgccgttgg tttcctgacc aagataacca tgacttaatg gaatacgttc
     4681 atccagttcg cgcgccggta attggcctac gcgcagttgg gtagtaacga aatcagcatc
     4741 tttcaatgct tcgcggcgat ccagcgtttt ataaagcttc atcgggacgc cagcgttatc
     4801 aatcatccgt tggcagagat cgaaaataat atccagttta gctttgccac cttcgacatc
     4861 caccagccat aattcgctga ccggcaattc gtgataacgc ttaataaatc cttccagtaa
     4921 ctccggggta tagctgctcc cgccaccaat agtgacgact tttaatttct ggctcataat
     4981 ttctcccttc agtacagaat actgatatct gacatatctg ccccccggac ataaataatc
     5041 cagcaacagg acagatatgt gaattgtcag gtataacgac ttactgatta aattcagtca
     5101 atttcttacg atagctctta ggcgtaaagg acgttaattt cttaaacgtt ttaataaaca
     5161 aactcgggct actataaccg gcctcaaacg caatatccgt cactgaatag ttggtcattt
     5221 ccagttgttt tttggcaaaa ttaatacgga tttcattaat aatctgcatt ggcgttttgc
     5281 cataatatcg ttgagtcgct cgcgtcaaat attcctgtga tttggctgac aacgtcacca
     5341 tattctccag cgccgattca ctaaactgct ctttatcatg catcttttct accgtacttt
     5401 tcagccactg cggtacatca tcaatcacct gttcttcgcg gtaatgacgt aaacggttaa
     5461 tgacataaaa agtaaccatc tcaacaaact cttccagccc tgtttcgcgg aaattcaatg
     5521 aagaaatcac tgtttccaca taggtgagaa aagcgttatt ggtccggtat acctgcgaag
     5581 ctacaaagca ataaggcaac aacggcaggt aatgctgctc aaaaaagcgt ttactgatcc
     5641 caacgttcaa tatacgcgtg gcaccaaact cataaaaact ttgatggtgc gaacctaacg
     5701 gaataaaaac aaaatcgccc cgttccagta acacgcgctt accgttaatc tcctggaaat
     5761 aacgcccggt taataccaga gtaaattcat aatagtcgtg ctgatgcagt ccgctgatac
     5821 tctcagtttt gttatagata aacacatgga agtttttgcc attaaacaac tgctgttctc
     5881 gggcagtggc aatttccggc gcgttaatca ctggctgcat catcgactcc ttatgccttc
     5941 agtttttcat gaagctcaat taattctgta atcagttcac gcgcaagcat ggacgtcatt
     6001 aaatgatcct gagcgtggac cagcaccaga ctcactttca tcttaccttc gcccgcatcg
     6061 ccttcaatca gtttcgtctg taccagatgc gcttcattca atgccatccg tgactgatcc
     6121 atcatggctt ttgctgcggc aaaatcgccc tgcttcgcct gtttcagtgc cgcatacgcc
     6181 aggctgcgcg cttgtccgga gttgatgata agccccatca ccacttcttc cagctcttca
     6241 gcttccgttt gcgtatcggg aatattatcg agatccatca tatatcgttc ctctctattc
     6301 ttaccggcac gattacccgt accggcatcg attaaaattt cagggcgtta gcgatatctt
     6361 cttcgctctc ttctttatca atcgcgtttt gtgctttgtt agccaccaca acaaagggca
     6421 gataaattaa cgttgcgata ccaaggttga agagtgcaac cagcaatgcg gcgacgctac
     6481 cgttggtgtt aaagaaggct cccagaccgg ttggcatggt ccacggtgca atattggtca
     6541 ccggaggaat aatgcccatg tagtacgctg ccagggtgat tgccgccaga atcggttgta
     6601 ccagtacaaa cgggataaac atcaccgggt tcataataat tggcagacca aacagaatcg
     6661 gttcgttaat ctggaagatg ccggacggca gcgccagttt tgccacctga cgataatcag
     6721 cacggcgaga ggcgataaaa atagccagga tcaggcctaa agtcgcacca ctgcccccaa
     6781 ggaaaataaa ggagtccagc atcggcttgg cccagatatg gaaggtctta ccggctgcca
     6841 gcgccgcttc gacggaacca tattgctgat aggtcgcgat attttccagc gcccacggcg
     6901 tcataatgcc gttgtccagt gcggtcagcg ccagcgcgcc atgaataccg aagaaccaga
     6961 gcagtggaac aaagatcaca taggcccagc ccaccacgct acccaacgat gccagtgggg
     7021 ttgagatggt atccataatg atctgatgga agttggtgcc ccaggtattc aacgcccagg
     7081 caataatccc catcacggaa agaataataa agccgggaat taatgccgag aaggaacgcg
     7141 atactgaagc aggtacgctg tcgggcagtt taatgaccca attgcggcgg acaataaagg
     7201 taaacatttc tgccaccacc aggccaataa taatcccgga gatgatattc gccccaccta
     7261 accagtttgc gccaaccgca taggcctcac cgacactata tggggtgacg gtcataaatg
     7321 ctgcaacgga taacaaccca gccgccagcg catcgacttt acgctcttct gccagcgcca
     7381 tgccaataaa gaacggtgcc attaaagaca ttatccctaa tgttccgtta tatacgttgc
     7441 cgccaatacc tttcagacca ttaagtgttt caatggttga ggcgtcgagg cgaataccta
     7501 aggaataaaa aaacgacccc tccccaaagc ttagaaaaac gttgttaatt aatacaaaca
     7561 tggcccccgc aagggttaac ggcattaagc gaataaagcc atttttgatt gcattaacgt
     7621 gtggctgctt tcctatttta actgcaaaag ggaggagtac cttttcaagc gatgcaataa
     7681 cattac
//
LOCUS       DAZKFU020000220         7684 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZKFU020000220 DAZKFU020000000
VERSION     DAZKFU020000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.222"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2151
                     /gene="tssH"
                     /locus_tag="ACHOQQ_RS19315"
                     /old_locus_tag="pgaptmp_003863"
                     /gene_synonym="clpV"
     CDS             <1..2151
                     /gene="tssH"
                     /locus_tag="ACHOQQ_RS19315"
                     /old_locus_tag="pgaptmp_003863"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGU4472066.1"
                     /translation="GADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPVSGRDT
                     EIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLRTLDLG
                     LLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAANLLKP
                     ALARGELRTIAATTWSEYKQYFERDAALERRFQMVRVDEPDDETACLMLRSLKSRYAE
                     HHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQLTRIRS
                     QLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENELMTARDDLEQQYARECELTGEL
                     LESRSDISRQSETHHLQQALHDIQQNQPLLSVDVDVRTVAGVVADRTGVPLSSLMKDE
                     QTELLHLEKDIGRRVVGQDVALESIAQRLRAAKTGLTSGNGPQGVFLLVGPSGVGKTE
                     TALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRPY
                     SVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDLLMQQL
                     SEKPETTESELHELIRPLLRDHFQPALLARFRTVIYRPLTQSAMRTIVEMKLAQVCER
                     LHCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYLAARQH
                     PTYLSLTWNDEDGIVIAFDGINSDYPQESIYGQC"
     gene            2138..2779
                     /locus_tag="ACHOQQ_RS19320"
                     /old_locus_tag="pgaptmp_003864"
     CDS             2138..2779
                     /locus_tag="ACHOQQ_RS19320"
                     /old_locus_tag="pgaptmp_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472067.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTGHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            3082..5601
                     /locus_tag="ACHOQQ_RS19325"
                     /old_locus_tag="pgaptmp_003865"
     CDS             3082..5601
                     /locus_tag="ACHOQQ_RS19325"
                     /old_locus_tag="pgaptmp_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGU4472068.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLELALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            5598..7280
                     /gene="tle1"
                     /locus_tag="ACHOQQ_RS19330"
                     /old_locus_tag="pgaptmp_003866"
     CDS             5598..7280
                     /gene="tle1"
                     /locus_tag="ACHOQQ_RS19330"
                     /old_locus_tag="pgaptmp_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HGU4472069.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSIA
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PAFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFAPIDPKNPDMNLSPE
                     LIPFVDKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            7300..>7684
                     /gene="tli1"
                     /locus_tag="ACHOQQ_RS19335"
                     /old_locus_tag="pgaptmp_003867"
     CDS             7300..>7684
                     /gene="tli1"
                     /locus_tag="ACHOQQ_RS19335"
                     /old_locus_tag="pgaptmp_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HGU4472070.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWNEHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIF"
BASE COUNT         1978 a         1645 c         2125 g         1936 t
ORIGIN      
        1 ggtgcggata cggcgggtat accgtcaggt gacctgccgc ctgtgttaca gagtgctctg
       61 gataaattca cccgggacat cacggccagc gcgagagagg ggaaaattga cccggtatcg
      121 ggacgcgata cggaaatccg tcagatggtg gatattctct cccgccgccg caagaacaac
      181 ccgattctgg tgggggaccc tggtgtgggg aaaacggctc tggtggaagg gctcgccctg
      241 cgtattgcgg aaggtaacgt accggaatca ctcagacccg tcaccctgcg cacccttgac
      301 ctcggcctgc tgcaggccgg tgccggcgtg aagggtgaat ttgaacagcg cctgaaaaac
      361 gtgattgatg ccgtgcagca gtcaccggct cctgtactgc tgtttataga tgaagcccat
      421 acccttatcg gggccggtaa tcaggccggc ggcgcggatg cggccaacct gctgaagcct
      481 gcgctggcgc ggggtgaact gcgtaccatt gccgccacca cctggtctga gtacaaacaa
      541 tactttgaac gtgacgcggc tctggagcgg cgttttcaga tggtcagagt ggacgagccg
      601 gatgatgaga cggcctgtct gatgctgcgc tccctgaaat cccgttatgc ggaacatcat
      661 aacgtgcata tcaccgatga ggcggtacgt gctgccgtca cgctgtcgcg ccgttatctg
      721 acagaacgcc agttaccgga caaggctgtt gacctgctcg atactgctgc tgcccgtgtg
      781 cgtatgagcc tcgacacggt gccggaacag ctgacccgta tccgttcaca gctcgcttcc
      841 ctcggtatgg aaaaacaggc actgctggaa gatattgccg ttggtcatca gaatcacggc
      901 gaacgtctgt ctgccattga gcaggaagag aacgagctga tgacagcacg tgatgacctg
      961 gagcagcagt acgcccggga gtgcgaactc accggtgagt tacttgaaag ccgcagcgat
     1021 atttcccgcc agagtgagac acatcacctg cagcaggcac tgcacgacat tcagcagaat
     1081 cagcccctgc tcagtgtgga tgttgacgta cgcaccgttg ccggtgtggt cgcggaccgg
     1141 accggggtgc cgttatcttc cctgatgaag gacgaacaga cggaactgct gcatctggaa
     1201 aaggatatcg gcagacgggt ggtcggacag gacgtggcac tggaatccat tgcgcagcgt
     1261 ctgcgtgcgg cgaaaacggg tctgacatcc ggtaacggcc cccagggggt gttcctgctg
     1321 gtcggtccca gtggtgtggg aaaaaccgaa acggcactgg cgctggcaga cgtgatgtac
     1381 ggcggcgaaa aatcactgat taccatcaat ctgtcggaat accaggagcc ccatacggtt
     1441 tcccagctta aggggtcacc gcccgggtac gtgggatacg gtcagggcgg tattctgacg
     1501 gaggctgtgc gtaaacgccc ttacagcgtg gtgctgctgg atgaagtgga aaaggcccac
     1561 cgggatgtgc tgaatctgtt ctaccaggtg tttgaccggg gctttatgcg cgacggtgag
     1621 gggcgtgaaa ttgacttccg taatacggtc attctgatga cctccaatct gggcagtgac
     1681 cttctgatgc agcaacttag cgaaaagccg gagacaacgg aatcggagct gcatgagctt
     1741 atccgtccac tgctgcgcga ccacttccag ccagcactgc ttgcccgttt ccggaccgtg
     1801 atttaccgtc cgctgacgca gtctgccatg cgcaccattg tggagatgaa gcttgcacag
     1861 gtctgcgaac gcctgcactg ccattatggg ctgagcacat cggttgatga acgtgtgtac
     1921 gatgccctga catccgcctg cctgctgccg gatacgggcg cccggaatgt ggagagtctg
     1981 ctgaaccagc aactgctgcc ggtactgagc cggcagttgc tcagctatct tgctgccaga
     2041 cagcatccga cgtacctgtc tctgacgtgg aatgatgagg atggtattgt gatagcgttt
     2101 gatggaatca atagcgatta tccgcaggaa agcatatatg gacagtgcta aagaaattgc
     2161 ccgggatatg ctggaggccg taaatattaa tggggatggg tttctggggg ccgtcgggaa
     2221 gggactcata tctttgccag tcagtctcgg ttatatggga tatgatttta tagataccgg
     2281 gcataggcgg gaaaaccaga atgacaaatt cagaatggcc agactggtag aaaaaggcat
     2341 tttcagccgt gatattattg ggaaaattat cagtatttgt cttgaagact ttgtatcccg
     2401 gatcaatatg gaaaaaacag gctccattgt gaagaacgtt tcaggagatc tggcaggtag
     2461 aatggcattt gctgaattga ccggggttaa actgggagag gttctggtat ccagagggct
     2521 ggctgctttt tttgcaggga gttttgccgg gttgttatta tctgttggcg cagagacatc
     2581 gcgtgcgatt tacacatccc gttttctgaa agacagaaat ccggtgttgc atgataagct
     2641 gaaagctcag ggggatttgg atcttttgta ctttcttgtt gaagatattg tgcgcccgtt
     2701 tgaacgagcc tgtattgtcg cagaccgaaa ttcggctgag tttgaccgga tatgcgaata
     2761 ttttttcggt ggtttatgaa gataaaaaga acattactta taatgctttc ccgatttatc
     2821 cgtggcgcag gtatggggtt aggtgcgtcg ggtattgtat ttacagtctg gttctttttt
     2881 ctctcatctg ctgaatttaa atatctgtgg ggagggctct ctgttgcgtt attttttgtc
     2941 ggttatctga tgtatcgatt tgcatatgca tatatttacg atgaatggga tgattatcag
     3001 taattctctc tgaaaataaa gcttaacggt catcattata taattgcagt tcaataccgg
     3061 tacagacatg gagttaacaa tatgagtctg acagatacac tttcaaaaac tatctctgct
     3121 tccctgaatc gttaccattt aattattccc tcctgcccgt caccactgga tgttgagcat
     3181 ttcagcggca gggaggagat gagtcagacc tattattaca tcatcaattt taccagtact
     3241 gacaacgacc tggatgccgg gctgctgctg cgtaaatcag ccacatttac aatgggtacg
     3301 ggagggctta aggagcaggt gagccagaaa gtagtacatg gtgtcattac agattttcgt
     3361 cgtatatcgg gctccgccga ccaggcaaaa taccaggtga tactggaacc atttattaaa
     3421 ctactggaca ggcagcatcg ttctcaccga tttttcgtta acaaatcggt gccggaggtg
     3481 gtgacagagg ttctccagga gcacggcctg aaaggctggg agtttgagtt ccgtctcaaa
     3541 aaaacgtatc cgaagcgtga acaaattaac cagtatcagg aaagtgacct gcaatttatt
     3601 cagcgtttgc tggctgaagt ggggattttt tattttttca ccctgcaacc ggacacgcag
     3661 acggaagtag tgcattttgg tgacagccag gctgcgctga cgtttgataa gacgctggcg
     3721 ataaacagtc cgtccggaat gaatgataac agagcggatt cagtgtgggg gctgaatgtg
     3781 acgcacaatg tggcagaggc cagtgtcacc acaaaggctt ataaccatcg tgaagcacaa
     3841 tatctgttgc agtccgcccc ggcagacatg acgcatggtg atggtgacgg aattaactat
     3901 ggtgaggtgt accactacca tttccgtcat ctggaacggg gggataaaat cgacccggtg
     3961 ccggagacgg ccaattttta tgcccgcctt ggtcatgaac gttatctggc ggagcaggtg
     4021 cggataaccg gcaacagtac agacgccacg ctggcacctg ctcaggttct gactatcacc
     4081 gacagtcttc cgccgacgtt gcctgcgctg ctgcgcaatc cggtgttgct tacctgtgtc
     4141 ggtttttctg caagtcgcaa ggatgccctg caggttgtac tgaagggcgt tccttacagt
     4201 gaagttatct gctggcgtcc tcctttactg ccgcgtccga aagttaccgg taccatgacg
     4261 gcccgtgtga ccagtgcaaa agaaggggat atctacgcct ggcaggatgc atcaggtatg
     4321 taccgggtga aatttgatgc tgaccgtgat gataaaaatc cgggacagga aagcatgccg
     4381 gtacgtctgg ccaaacccta cagtggtgat gcttatggtt ttcattttcc gctgattcag
     4441 ggaacggaag tggccattgc ctttgaggaa ggtgatccgg atcgccccta tatcgcgcat
     4501 gccctgcatg attcacgtca tgttgatcac gttaccgata agaacggtac gcgcaatgtt
     4561 atccgtacgc cggcaaataa caagttgcgg atggaggaca aacgcggtga ggagcatatc
     4621 aagatcagta ccgagtttgg cggtaagaca caacttaatc ttggtcataa cgttaatgcc
     4681 tcccggaaac tcaggggaga aggcgctgag ctgaggactg atgactggat aagtatccgg
     4741 ggaggaaaag gaatatttat cagtgcagat atgcagcctc aggcacaagg aaagatgctg
     4801 gatatgaatg aagctatccg ccaactggag ctggcattat ctctggcccg aagtatggcg
     4861 aaggccgcaa ctgctgcaaa tgctactcag ggagatataa gttgtcagca acggttgaat
     4921 tcatcgctaa cagatctcac tgcgccggga atgctgttgc atgctcctga tgggattggc
     4981 atggttagtg cacgggcgtt acgaattgct tcgggtagtg aaagcgtggg cataatgtcg
     5041 ggagataaca ctgatattag tgccgggcag tcatttacag ttgtagcaga aggtgctgtt
     5101 agcctgttga gcagaaacca ggggatgcaa ctgttagcag caaaagggcg ggtaaatatt
     5161 caggctcaaa gtgacgattt gtcaatgagt tcccaacaga atcttgatat tcaaagttct
     5221 gagggcaaag tgactgtttc tgctaatcag gaactgatac tggcatgtgg tggagcttat
     5281 atcaaactaa gcggtggaaa tattgagctg ggatgcccgg gacagatcct gttaaaaagc
     5341 acgaatatgc agaagatggg accaacaagt cttgatattg cgtctgttga aatgccgcga
     5401 gggtttggtg gcggttttat tcttaccgat gaagcgggag tgccgcaacc ttcgacgcct
     5461 tatcgattaa caacggctga aggtgatatt ctacagggga tcaccgatga aaatggtaaa
     5521 acagcgccag tcaacacctc cattccgtca gtagtaaaag ttgagtttgg gaaggtaaaa
     5581 attcatggag aaacagaatg acaaaatatc agggatatga tgtaacagat gccacccata
     5641 aaaccagtat tcataatgac tggaaagttg ttgttgcaaa gaaaaaaccg gcgcggggtg
     5701 tgactttaac cattggtata tttttcgatg gtaccggaaa caatcgggag aatacagcat
     5761 ctcgtctgat gaaatttaat gagtgtagtg cagccagaca gggggtaaat caaaaagatg
     5821 cacaaagctg tgaggacttt cttgaagaaa ttaacaagaa cagtatctcg aatggcagtt
     5881 atcgtggata ttattccaat atccactggc taaattcact ttacgtagca gatcagactt
     5941 tgttggaaag tcaggttgaa gcacaaattc agacttacat ttccgggata gggactgcgg
     6001 ctggtgaagc agattcagtc attggcatgg gattaggcac cagtattctg gatatatttg
     6061 aaggtgtggt gacgaaaacc gacgaggcaa tggaacgaat cacccaagcg ttatcagaat
     6121 ttatggggtt caatttaaat cctgattttt gtatcgcaaa aatccagttt gatgtttttg
     6181 gttttagccg gggagcagcg gcggcaaggc actttgctaa tcgggttatg gaacaggatc
     6241 ctgctatagc cagagctatt gctaaagggc tcagaggcga tttttatgat ggtaaacctt
     6301 cgggtgaagt ccgttttctg gggctttttg atactgtcgc agctattggt ggaatcagta
     6361 atttcttcga tattaatgga cgtagcaacc cgggagtaaa gctggaattg cgtccttcca
     6421 tcgcgaagaa agttttccag attactgcaa tgaatgaata ccgatataat ttcagcctga
     6481 acagtattaa aggtatgtgg cccgaactgg ctttaccagg ggcgcattca gatattggag
     6541 gtggttacaa tccggtaggt tcacctctgc aggaaaatga aagtctgttt ctgagttgcc
     6601 cggcatttga aattgtcagt gacgatacca gagaaacaga tacgcgagta taccgtaatg
     6661 cagagcaagc cagaaaaatg ttgatgactc tgccagcctt aaaacatatt ttgcctcatg
     6721 gaaaactaac caccaaaatt cgttccgtag gtgtgaacaa cagcaatcaa cgtcgggctg
     6781 gcgtgataca aaagcaggtg ggagccgcag tattttttga gcgtatggca gtacctaatg
     6841 actgggctaa tgtttgcctg cgtgtgatgc tggatgctgc tcaggaagca ggggtattgt
     6901 ttgctccaat tgaccccaaa aatccagata tgaatttatc tcctgaatta attccttttg
     6961 ttgataaagc cattgctcag ggaaaagcgg tacgtttggg gcaagagcct caggctttta
     7021 ctgaggaaga gttgtatatt atcggaaaat acacccattg ttctgccaac tggaatattg
     7081 agtctgatgg taatttatgg gtggatccta caacgggaga gatttttatc catcgctttg
     7141 gtcctaaagg taataaggcg ttcgtttttc cgaataaacc aaatgatcgc tggattcgtt
     7201 ctgtatggta catggatgat cagcagcgat tgaatgataa cgcagtaaaa aatactaaag
     7261 ttatgatgtc tggtgtttaa cgctacagga tgacaataca tgaccagaga taaaatcatt
     7321 tttatcgcac cattattggc attgttaact gggtgtaatt ctgttagctc acagtatgct
     7381 gattcggata agactgaatg gggttataaa aaattaccct atgatgaatg gaaatttctc
     7441 tttatttatc caaaggctct gcctgcgctg gccactcagg cattgatggt ggacggggaa
     7501 ggttataaga cgacttatca gtatcttgat accacaagac cgagtcaggt cagtgtaggg
     7561 cgctggaatg agcatctggg cgcgactgag tcgtattata acaaggggaa agcattacct
     7621 ctgatgttgc gtttttgctg ggattcgata attgataaaa agagttatga aactctgatt
     7681 tttt
//
LOCUS       DAZKFU020000221         7659 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZKFU020000221 DAZKFU020000000
VERSION     DAZKFU020000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.223"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..540)
                     /locus_tag="ACHOQQ_RS19340"
                     /old_locus_tag="pgaptmp_003868"
     CDS             complement(<1..540)
                     /locus_tag="ACHOQQ_RS19340"
                     /old_locus_tag="pgaptmp_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4472071.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQD"
     gene            1018..1806
                     /gene="fabI"
                     /locus_tag="ACHOQQ_RS19345"
                     /old_locus_tag="pgaptmp_003869"
     CDS             1018..1806
                     /gene="fabI"
                     /locus_tag="ACHOQQ_RS19345"
                     /old_locus_tag="pgaptmp_003869"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGU4472072.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSNIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1950..3077
                     /gene="yciW"
                     /locus_tag="ACHOQQ_RS19350"
                     /old_locus_tag="pgaptmp_003870"
     CDS             1950..3077
                     /gene="yciW"
                     /locus_tag="ACHOQQ_RS19350"
                     /old_locus_tag="pgaptmp_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGU4472073.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISTLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            3145..5079
                     /gene="rnb"
                     /locus_tag="ACHOQQ_RS19355"
                     /old_locus_tag="pgaptmp_003871"
     CDS             3145..5079
                     /gene="rnb"
                     /locus_tag="ACHOQQ_RS19355"
                     /old_locus_tag="pgaptmp_003871"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGU4472074.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(5198..5260)
                     /locus_tag="ACHOQQ_RS19360"
                     /old_locus_tag="pgaptmp_003872"
                     /pseudo
     CDS             complement(5198..5260)
                     /locus_tag="ACHOQQ_RS19360"
                     /old_locus_tag="pgaptmp_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5314..7299
                     /gene="pdeR"
                     /locus_tag="ACHOQQ_RS19365"
                     /old_locus_tag="pgaptmp_003873"
     CDS             5314..7299
                     /gene="pdeR"
                     /locus_tag="ACHOQQ_RS19365"
                     /old_locus_tag="pgaptmp_003873"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4472075.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(7281..7409)
                     /gene="ymiD"
                     /locus_tag="ACHOQQ_RS19370"
                     /old_locus_tag="pgaptmp_003874"
     CDS             complement(7281..7409)
                     /gene="ymiD"
                     /locus_tag="ACHOQQ_RS19370"
                     /old_locus_tag="pgaptmp_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGU4472076.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            7447..7620
                     /gene="yciZ"
                     /locus_tag="ACHOQQ_RS19375"
                     /old_locus_tag="pgaptmp_003875"
     CDS             7447..7620
                     /gene="yciZ"
                     /locus_tag="ACHOQQ_RS19375"
                     /old_locus_tag="pgaptmp_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGU4472077.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
BASE COUNT         1880 a         1927 c         1866 g         1986 t
ORIGIN      
        1 atcctgatat atttcctgca gatgttgtgg atcgcttttt tgcggtgtta aacgacgata
       61 aaccgtacct tccatcatgg tagcaaacac ttcaacgcag caacgaatgt gctcgtcgct
      121 aagatgggga tgcattcttt tcatatgtgc acaaccgtta gcgaacattc tggcgtcagc
      181 ttcttcaagc attgccataa cccgcgggtt gcgtgtggct tctgcagcca cttccagcat
      241 cagcgcgtcg tcgtcctcat ttaaggattt acgcaaggca aggacttccg ggaagtgatc
      301 ggtacgcgcg tcaatgtcca tttgggcaat acgaaagtcg atgatacggc gaaccatctc
      361 ttcaataatg gcgtctttgt tggcgaagta acgatatatc tgacccacgc taagctgagc
      421 ttcgctggca atttgcgaca tactggcggc atgaaacccg cagcgacgaa aacaccgacg
      481 ggcggcgtta atgatttcat cctgacgctg tttgtggcgt atctccagct tgctggtcat
      541 ttctgtctcc tggcctatca cttggtgaga atgatcgttc tcatttttgc tgattatacg
      601 tttctccaaa gtaatggaaa gtatttttat cttattcatg gtgcatattc tgataatgaa
      661 gagattgtgg agattctgtg aagatagttg acaatattaa gttggggtca attcatcaga
      721 aaggctgcta cttgttgtcg ccagttctgt tttcatcatc aaacttatcg gagctaatac
      781 gaatagtttc atagtcagat tctataagat attttcccaa cctacccctt gcaggcgtca
      841 agagtagata acgcaaaatg ctctgtaccg cagcttctct ccggtacaga aagcgcaact
      901 atagccaccc acagccaggt tgattataat aaccgtttat ctgttcgtac tgtttactaa
      961 aacgacgaat cgcctgattt tcaggcacaa caagcatcaa caataaggat taaagctatg
     1021 ggttttcttt ccggtaagcg cattctggta accggtgttg ccagcaaact gtccatcgcc
     1081 tacggtatcg ctcaggcgat gcaccgcgaa ggagctgaac tggcattcac ctaccagaac
     1141 gacaaactga aaggccgcgt agaagaattt gccgctcaat tgggttccaa tatcgttctg
     1201 caatgcgatg tggccgaaga tgccagcatc gacaccatgt tcgctgaact ggggaaagtt
     1261 tggccgaagt ttgacggttt cgtacactct attggttttg cacctggcga tcagctggat
     1321 ggtgactatg ttaacgccgt tactcgtgaa ggcttcaaaa ttgcccacga catcagctcc
     1381 tacagcttcg ttgcaatggc aaaagcttgc cgctccatgc tgaatccggg ttctgccctg
     1441 ctgacccttt cctaccttgg cgctgagcgc gctatcccga actacaacgt tatgggtctg
     1501 gcaaaagcgt ctctggaagc gaacgtgcgc tatatggcga acgcgatggg tccggaaggt
     1561 gtgcgtgtta acgccatctc tgctggtccg atccgtactc tggcggcctc cggtatcaaa
     1621 gacttccgca aaatgctggc tcattgcgaa gccgttaccc cgattcgccg taccgttact
     1681 attgaagatg tgggtaactc tgcggcattc ctgtgctccg atctctctgc cggtatctcc
     1741 ggtgaagtgg tccacgttga cggcggtttc agcattgctg caatgaacga actcgaactg
     1801 aaataatcgt tctgttggta aagatgggcg gcgttctgcc gcccgttatc tctgttatac
     1861 ctttctgata tttgttatcg ccgatccgtc tttctcccct tcccgccttg cgtcaggata
     1921 acgatttcct ttacgaccaa ggagcgccca tggaacaacg ccacatcacc ggcaaaagcc
     1981 actggtatca tgaaacgcaa tccagtacta cggagtatga cgttctgcct ctggtcccgg
     2041 aagccgcaaa ggtcagcgat ccctttctac tcgacgtgat ccttgaaaaa gaaacgctgg
     2101 cccccttcct ttcatggctg gaccctgcgc gtgttcttgc agtggagttg ttccctgacc
     2161 agcttaccgt gacccgttca cagaccttca ccgcttatga acgcttgtcg acggccctga
     2221 cggttgctca ggtttgcggc gtccagcggt tatgtaacta ctattcggcg cgacttacgc
     2281 cgctccccgg gcctgattcc accagggaaa gtaatcatcg gttggcacaa atcacgcaat
     2341 atgcccgcca actggctagc tcgccttcta ttatcgacaa ccgatcgcgc cagcatctga
     2401 atgacgtcgg tcttactgcc tgggactgtg tgatcattaa ccaaatcatt ggttttattg
     2461 gctttcaggc gcggacaatt gcgacatttc aggcttatct cgggcatccg gtacgctggt
     2521 tacccgggct ggagatacaa aactacgccg acgcgtcact gtttgctgat gaatcattac
     2581 gctggcgaag cagctatgag gtggaaaaac tacctgaaga acacacaaaa agttcaactg
     2641 cagaactttg ccaactggcc gaaatactct ctctccaccc tatttcactt tcccttctcg
     2701 aaaggttgtt aaacagcaca cgggttaata cacagccgga taatcagctt gcggcgttgt
     2761 tatgcgcgcg gataaatggc agtcctgctt gttttgccgc ctgtatggat tcatcaaatg
     2821 aatataaaaa aatcagcacc cttctgcgca agggcgaaaa tgaaattaac caatgggctg
     2881 accgtcattc tgttgagcgc gctaccgttc aggcgataca atggctgacc cgagcacccg
     2941 atcgctttag cgccgcccag ttcagccctt tactcgaaca cgaaaaatca tcaacgcaga
     3001 ttattaatct gctggtatgg agcgggctgt gtggctggat aaatcgctta aaaatcgcgt
     3061 tgggtgagac atattaacct tgccgcgtca gacagattcg cgtaaaactg tcagccgctc
     3121 taatggccac caaaatagac aattatgttt caggacaacc cgctgctagc gcagcttaaa
     3181 cagcaactgc attcccagac gccacgcgct gaaggggtgg taaaagccac agaaaaaggc
     3241 tttggcttcc tggaagtcga cgcgcaaaaa agttatttca ttccgccgcc acagatgaaa
     3301 aaagtcatgc atggcgaccg aattatcgcg gtgatccaca gtgaaaaaga acgtgaatcc
     3361 gcagagccag aagaactggt tgaaccgttc ctgactcgtt tcgtgggtaa agttcagggc
     3421 aaaaatgacc gtctggccat cgttcctgat catccacttt taaaagacgc cattccttgc
     3481 cgcgcagccc gtggtctgaa ccacgagttt aaagaaggcg actgggctgt tgccgaaatg
     3541 cgccgtcatc cgctgaaagg cgatcgttct ttctatgcag aactgacaca atacatcact
     3601 tttggtgacg accactttgt accgtggtgg gttacccttg cgcgccataa tctggaaaaa
     3661 gaagcgccag acggcgtcgc taccgaaatg ctcgatgaag gtctggttcg tgaagatctg
     3721 accgcgctgg attttgtcac catcgacagt gccagcacag aagatatgga tgacgccctt
     3781 ttcgctaagg cgttgccgga tgacaaactt cagctgattg tggcgattgc cgatccaacc
     3841 gcgtggattg ctgaaggcag caaactggac aaagccgcga aaattcgcgc attcaccaac
     3901 tatctgcctg gcttcaacat ccctatgctg cctcgcgagc tttctgacga tctctgctca
     3961 ctgcgcgcca atgaagtccg cccagtactg gcatgccgca tgacgctctc cgctgatggc
     4021 accattgaag ataatatcga attctttgcc gccaccatcg aatccaaagc gaagctggtg
     4081 tatgaccagg tttctgactg gctggaaaat accggtgact ggcagcctga aagtgaagca
     4141 attgccgaac aagtccgttt gctggcgcaa atttgccaac gacgcggcga gtggcgtcat
     4201 aaccacgcac tggtgtttaa agatcgcccg gattaccgct ttattctcgg tgaaaaaggt
     4261 gaagtgctgg atatcgtcgc cgagcctcgt cgcattgcca accgtatcgt cgaagaagcg
     4321 atgattgccg ctaacatttg tgcggctcgc gtactgcgcg ataagctcgg ttttggtatc
     4381 tataacgtgc atatgggctt tgatccggcg aatgccgacg cgctggcagc gttgctgaaa
     4441 acgcacggtc tgcatgtcga tgccgaagaa gtgctcacgc tggacggttt ctgcaaactg
     4501 cgtcgtgaac tggatgcgca accaactggt ttcctcgaca gccgcattcg tcgcttccag
     4561 tcatttgctg aaattagcac tgaacccggt cctcactttg gcctcggtct ggaagcatac
     4621 gccacctgga cttcgccgat ccgtaaatat ggcgacatga tcaaccaccg tctgctgaaa
     4681 gcggttatca aaggcgaaac tgcgacgcgt ccacaggatg aaatcactgt gcaaatggcc
     4741 gagcgtcgcc gtctcaaccg gatggcagaa cgtgatgttg gtgactggtt atacgcacgc
     4801 ttcctgaaag acaaagccgg gaccgacacc cgtttcgcag cggaaattgt cgatatcagc
     4861 cgtggcggca tgcgtgttcg tttggttgat aacggcgcta tcgcctttat tcctgcacct
     4921 ttcttacacg ctgtgcgcga tgaactggtt tgcagccagg aaaacggcac cgtacaaatt
     4981 aaaggtgaaa cggtttataa agtaactgac gttattgacg tcaccattgc cgaagtccgc
     5041 atggaaaccc gcagcattat tgcgcgcccg gtcgcgtaat ctcctttcac ggcccattcc
     5101 ttatgaatgg gccgtttatt tccccgctct accttcatca tatcccggca gttttttaac
     5161 tgactttcgt ttgaaaactg gcgtttttcc aaaactagat tacaaataaa tatataaaaa
     5221 aaacaatgtg tttagccgcc gttaatttca tgtaagtcat gttgttcctt ttatttaacc
     5281 gcggactccg ctgttaaccg gaggatatgc atcatgaaaa ccgttaggga gtccacaacg
     5341 ttgtacaact ttctcggatc gcacaatcca tactggcggt tgacggaaag cagcgatgtt
     5401 ttgcgctttt ctaccaccga aaccacagaa cctgaacgta cgttgcagtt atctgccgaa
     5461 caggctgctc gcatcaggga aatgacggtc atcacctcca gcctgatgat gagtctgacc
     5521 gtcgatgaga gcgatctttc tgtgcatctg gtaggacgaa aaatcaataa acgggaatgg
     5581 gctggcaacg cgtctgcatg gcatgacaca ccggcagttg ctcgtgattt atcacacggg
     5641 ctttcctttg ctgagcaggt agtttctgaa gcacattccg caatagtgat tctcgacagc
     5701 cgggggaata tccaacgctt caatcggtta tgtgaagatt acacagggtt gaacgaacac
     5761 gacgtcattg gccaaagcgt gtttaaactg tttatgagcc gtcgtgaagc tgcggcatcc
     5821 aggcgcaata accgtgtatt ttttcgaagc ggcaatgcat atgaagtcga actgtggata
     5881 ccaacacgta aaggccagcg gctgtttctg tttcgcaata aatttgtcca cagcggcagt
     5941 ggcaaaaacg agattttttt aatctgttcc ggcaccgaca ttaccgaaga gcgccgcgct
     6001 caggagcgac tgcgtattct ggcaaatacc gacagtatca ccggactgcc gaatcgtaac
     6061 gcaatgcagg agttaatcga tcacgctatt aatcatgcag ataacaataa agttggggtt
     6121 gtgtatcttg atttggataa ttttaaaaag gtcaacgacg cctatgggca tttgtttggt
     6181 gaccagttat tacgcgacgt gtcattggct attttaagct gtctcgaaca tgaccaggtg
     6241 ttggcgcgtc caggtggcga tgagtttctg gtactggcat ccaacacctc acaaagcgcg
     6301 ctggaagcaa tggcatcacg aattttgacc cgcttacggc tcccctttcg cattggttta
     6361 attgaagttt ataccagctg ttcagtaggt attgcactct ctcccgaaca tggttcggac
     6421 agcgcggcta ttattcgtca cgccgacaca gcaatgtaca cagcgaagga aggcggacga
     6481 ggacaatttt gcgtttttac tccagaaatg aatcagcgag tatttgaata tctctggctg
     6541 gataccaact tgcgtaaagc actggaaaac gatcagttgg ttattcacta tcaaccgaaa
     6601 atcacctggc gtggcgaagt gcgcagtctg gaagcactag tacgttggca gtcacctgaa
     6661 cgtgggttga ttccaccgtt ggacttcatt tcctacgcag aagagtcagg gctaattgtg
     6721 cctttaggcc gttgggtgat tctcgatgtc gtacgccagg tggcgaagtg gcgggataaa
     6781 ggcattaacc tgcgagtggc ggtaaacatt tctgcacgtc agctcgccga tcaaaccatt
     6841 ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc tattgatgtt
     6901 gaattgacag agagttgtct gattgagaat gatgaactgg cactgtctgt tattcaacaa
     6961 tttagccaac taggtgcgca agtgcatctg gatgattttg gtaccggcta ctcttcactt
     7021 tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt tgttcgagat
     7081 attcacaaac aacctgtctc gcagtcactg gttcgggcaa tcgtcgctgt ggcccaggca
     7141 ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc ttttttaacc
     7201 aagaacggga tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc cgccgtcgcc
     7261 ttcgaacgct ggtataaacg ctatctgaag cgcgcataag tagatgaata tatatactta
     7321 gcgctatatt ccgtcattat atatcgtatt cccggatgat aattagccgt tgctgttaaa
     7381 cacttctctt ttagcggcaa tttttgcatt attcattttc ccccttttgc tataaggatg
     7441 tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat tgtgttagat
     7501 atccttgtgg caggcgatta ccactctgcg atccataatc tcgagatact taaagcagaa
     7561 ctcttgcgcc aggttgctga atctacgcca gacatcccga aggcaccgtg ggagatttaa
     7621 caatcgtgcc ttcaggtttt cctatttgag gatgcccgg
//
LOCUS       DAZKFU020000222         7646 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZKFU020000222 DAZKFU020000000
VERSION     DAZKFU020000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.224"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..270
                     /locus_tag="ACHOQQ_RS19380"
                     /old_locus_tag="pgaptmp_003876"
     CDS             <1..270
                     /locus_tag="ACHOQQ_RS19380"
                     /old_locus_tag="pgaptmp_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472078.1"
                     /translation="PEKRINEVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDED
                     LEYGYLINTGNHYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAI"
     gene            563..1804
                     /gene="agp"
                     /locus_tag="ACHOQQ_RS19385"
                     /old_locus_tag="pgaptmp_003877"
     CDS             563..1804
                     /gene="agp"
                     /locus_tag="ACHOQQ_RS19385"
                     /old_locus_tag="pgaptmp_003877"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGU4472079.1"
                     /translation="MNKTLITATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(1842..2069)
                     /gene="yccJ"
                     /locus_tag="ACHOQQ_RS19390"
                     /old_locus_tag="pgaptmp_003878"
     CDS             complement(1842..2069)
                     /gene="yccJ"
                     /locus_tag="ACHOQQ_RS19390"
                     /old_locus_tag="pgaptmp_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGU4472080.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(2090..2668)
                     /gene="wrbA"
                     /locus_tag="ACHOQQ_RS19395"
                     /old_locus_tag="pgaptmp_003879"
     CDS             complement(2090..2668)
                     /gene="wrbA"
                     /locus_tag="ACHOQQ_RS19395"
                     /old_locus_tag="pgaptmp_003879"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4472081.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            complement(2665..3975)
                     /locus_tag="ACHOQQ_RS19400"
                     /old_locus_tag="pgaptmp_003880"
     CDS             complement(2665..3975)
                     /locus_tag="ACHOQQ_RS19400"
                     /old_locus_tag="pgaptmp_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4472082.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSQKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLGRQHQITVKLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNILNSRAPDVPQSDQEDEIKLYFSK"
     gene            complement(4028..4312)
                     /locus_tag="ACHOQQ_RS19405"
                     /old_locus_tag="pgaptmp_003881"
     CDS             complement(4028..4312)
                     /locus_tag="ACHOQQ_RS19405"
                     /old_locus_tag="pgaptmp_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4472083.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            complement(4358..4609)
                     /locus_tag="ACHOQQ_RS19410"
                     /old_locus_tag="pgaptmp_003882"
     CDS             complement(4358..4609)
                     /locus_tag="ACHOQQ_RS19410"
                     /old_locus_tag="pgaptmp_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4472084.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIS
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            complement(4974..5336)
                     /locus_tag="ACHOQQ_RS19415"
                     /old_locus_tag="pgaptmp_003883"
     CDS             complement(4974..5336)
                     /locus_tag="ACHOQQ_RS19415"
                     /old_locus_tag="pgaptmp_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4472085.1"
                     /translation="MNAVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGVVT
                     VKNGIWQAVAREVDKKPNIAPVQPVQPVQHNIIGDLLRKSRKEARRAGRKQKRWEGAC
                     KALQELNKYRDLINELSE"
     gene            complement(5372..5584)
                     /locus_tag="ACHOQQ_RS19420"
                     /old_locus_tag="pgaptmp_003884"
     CDS             complement(5372..5584)
                     /locus_tag="ACHOQQ_RS19420"
                     /old_locus_tag="pgaptmp_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU4472086.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSLTV"
     gene            complement(5544..6170)
                     /locus_tag="ACHOQQ_RS19425"
                     /old_locus_tag="pgaptmp_003885"
     CDS             complement(5544..6170)
                     /locus_tag="ACHOQQ_RS19425"
                     /old_locus_tag="pgaptmp_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HGU4472087.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            complement(6167..6598)
                     /locus_tag="ACHOQQ_RS19430"
                     /old_locus_tag="pgaptmp_003886"
     CDS             complement(6167..6598)
                     /locus_tag="ACHOQQ_RS19430"
                     /old_locus_tag="pgaptmp_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HGU4472088.1"
                     /translation="MKQIAFYRRSGRPGVFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARGLTKGSKVVKIKASEPFTTDTGIVDRFFSLESNPRRDTPRSRNAVPP
                     FSRRSREHAAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            complement(6654..7289)
                     /locus_tag="ACHOQQ_RS19435"
                     /old_locus_tag="pgaptmp_003887"
     CDS             complement(6654..7289)
                     /locus_tag="ACHOQQ_RS19435"
                     /old_locus_tag="pgaptmp_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HGU4472089.1"
                     /translation="MTSQLIPVFNGSISNQAALLCNARNLHAFLGVGKRFASWITERI
                     AEYGFVENQDYMIASQIREAKGRGGHNRKDYHLTLDTAKELAMVERNEKGRQIRRYFI
                     ECEKKFRQSLLPAPMNINYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMRI
                     LNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
BASE COUNT         1847 a         1781 c         1841 g         2177 t
ORIGIN      
        1 ccagagaaga ggattaatga ggtgaaaaat ttcctcactg ctctttcaga tagcatttgc
       61 tcattattta ttgttaatag taatgtggca tcaatctcct tggggaatga atccttttca
      121 acagatgaag atcttgagta tggttattta ataaacaccg gcaatcatta tgacgtttac
      181 ctccctcctg aactttttgc tcaggcttac aagttaaaca ataaggaaat gaatgcgcaa
      241 cttgactatt taaatcgtta tgcaatttaa tggcaaaggc atatgctaaa aaccattgtt
      301 attagtctca cactttttta ttgttaaata ttgtctctgt attggtaacg ccgcagatat
      361 tctgtttagc cacaggtgca attatcagcg gcgtacgcga ggcaggggct aatcaggcat
      421 agtttgcgtc aaaccttgcc tgtttttgaa gatgtatata gaaaaacagg cgttcaacaa
      481 gccattttgc gaacctgttc ccggaaaaaa gtcatatttc tgtcacactc tttagtgatt
      541 gataacaaaa gaggtgccag gaatgaacaa aacgctaatc accgcaactg tggcagggat
      601 agttttactc gcttcaaatg ctcaggcaca aaccgtaccg gaaggctatc agctacagca
      661 agtgctcatg atgagccgcc ataacttacg tgcgccgctg gcgaacaatg gcagtgtgct
      721 ggagcagtcg acgccgaata aatggccaga atgggacgtc cccggtgggc aactcaccac
      781 caaaggtggc gtgctcgaag tgtatatggg ccattacatg cgtgaatggc tggcagagca
      841 ggggatggtg aaatcggggg aatgcccgcc tccggacacc gtttatgcct atgccaatag
      901 tctgcaacgt actgtcgcga ccgcacagtt ctttattacc ggcgcattcc cggggtgtga
      961 tattcctgtg catcaccagg aaaaaatggg caccatggac ccaaccttta acccggtgat
     1021 caccgatgat tccgccgcat tcagtgaaca ggcggtggcg gcaatggaga aagagctcag
     1081 caaactccag cttaccgaca gctaccagct actggaaaaa atcgttaact ataaagattc
     1141 ccctgcctgt aaagagaaac agcagtgttc gctggtggat ggcaaaaata cctttagcgc
     1201 gaagtatcaa caagaaccag gtgtttccgg gccgctgaaa gtcggcaact cgctggtaga
     1261 tgcgtttact ttgcaatact acgaaggttt tccgatggat caggtggcct ggggagaaat
     1321 caaatctgac cagcagtgga aggtgttgtc gaagctgaaa aacggttacc aggacagcct
     1381 gtttacctca ccggaagtgg cgcgcaatgt tgcgaaaccg ctggtcagtt atatcgacaa
     1441 agccctggtc accgatcgca ccagtgcacc aaaaatcacg gtgttagttg ggcatgactc
     1501 caacattgcc tctctgttaa cggcgctgga tttcaaaccg tatcagttgc atgaccagaa
     1561 cgaacgcacg ccgattggcg gcaaaatcgt tttccagcgt tggcatgaca gcaaagccaa
     1621 tcgcgatttg atgaaaatcg agtatgtgta ccagagtgcg gaacagttac gtaatgccga
     1681 tgcgttaacc ctgcaggcac ctgcgcagcg tgtgacgctg gaattaagcg gttgcccgat
     1741 agatgccaac ggtttctgcc cgatggataa gtttgatagc gtgttgaatg aagcggtgaa
     1801 ataacagaaa actcccccgt gagaaacggg ggagtcgctg gttaaacgtt tttacgttct
     1861 atggtctgtt cgccccaaaa aagcgaatct ttatcggtct tagcaaaggc tttgactaac
     1921 acttcatcac taccttcttc ccaaatcttt tccgccattt tttcgtcgta cccggcgact
     1981 tcgaaaatgg cctcagctat ttccggcgac gtattgcgca gagatgccca ttcaccgacg
     2041 tgatgagctt tcgcttcttg agttggcatg cgtatcctcc tgttgaagat tagccattaa
     2101 gtttaactgc cagacctgcg acatattccc cttgataacg agcaatagac agttcttcct
     2161 ggctgggctg gcgtgaaccg tcaccgcctg cgatggtggt tgcgccgtac ggcgtaccgc
     2221 cgcgaacctg tgaaacgtca aataattcct gcgctgcgta gccaatgggg acaatcacca
     2281 tgccgtgatg cgcaagggtc gtccaggtgg atgtgatggt ttgttcctga ccgccgccag
     2341 taccggtgga actaaagacg ctcgccagtt ttccgtatag tgcgccggaa gcccacaggc
     2401 cgcccgtctg gtcgaggaag gtacgcattt gaccggacat gttgccaaag cgggtaggtg
     2461 taccaaaaat aatggcgtcg taatcggcca gttcttgcgg ggttgcaacc ggtgcagttt
     2521 gcgttttacc gcctgctttt tcaaataatt gcggcggcat ggtttccggt acacgcttaa
     2581 cgacaacttc agcgccatcc actttgcttg caccctcagc gactgcgcgt gccatcgttt
     2641 caacatgtct aaggatatgg tttatcattt tgaaaaatat aattttattt catcctcctg
     2701 atcactttgg ggcacgtctg gggcgcggga attaaggata ttattcaaca tagctacctg
     2761 agtcacgctg cactcaggca tccatgcacc ataaacattg tagaccatgc tggcgctgga
     2821 gtgccccatc tgtgacgcaa taaatgtcgg gtttgctccg gcagataaag cccagcacgc
     2881 ataggtatgg cgtgactgat acgctttacg ggatcggata cccgctcttt ttattgctga
     2941 atcccatgtc gctccgatgg agcttaccgc gtagttaata cccgccttgt gattcttgcg
     3001 aacgatttgc ggacagaaaa caaaagtgca ctcgtgcaaa attgttcttc cgtactcgcg
     3061 taatttaaca gtgatctgat gctgcctgcc aagacgagta agcatcgcct ggtttttaag
     3121 tgcttcaatt gctggtgcca gaagatgtat aacccggtta gtgcctgcgt cagtctttgg
     3181 tagcgtaaaa tcacctattt ttgtaaaatt tcttcgcact gttatcgtgc cagctttcag
     3241 gtcgatatcc tcccatgcaa gtgcggcaat ttcaccgtgt cgcatccctg taaaaacagc
     3301 cactgtccag aggtttttgg tctgttgatg atggcaggca tcaatgagac ggctaaactc
     3361 atctctggtc agtggatccg gcactggttt tgatttcctc agcggtgtta ttgaattaaa
     3421 cgggtttttc tccagatacc cgttttcggc ggcaaagctg aacattccgg ctgttgttgt
     3481 catgtaatag ttcactgtgg gtacggttct tcctttttgg gacgtgctgg ttttcctgct
     3541 tcccttttcc ccggtcagta aatctttcct gataaacagc agatcttctt ttgtaatcga
     3601 tgacgccagc ctgccaggac caagtctcgg tagcatattt ttcatcactg attgataacg
     3661 atttaaggcg ttactactga tttccattgc tttcagagta agccatttct gtgccagttc
     3721 accgacggtg atatcttttt ttaccaggcc aaatagcttc aggttaggtg agtcagggaa
     3781 tcgctcggca taatcaaacg ttcccgttct gattgcaaag cagacagaag cccttaactc
     3841 accagcgatc tttctgtttt tcggagtatc gggcacgcga agattttcgc gcactcgttt
     3901 acccctgtac ttaaacgtta ttcggagttt tcctccgtga ttttcaacgc cggctggata
     3961 ggctgaattc gccattgttc ctcctgcgtc caagagcacg ctcaggttat actcttatgg
     4021 acaagaatta aacatcctga gatggtaagg gttggttttt gatccaagtg ttgatggtag
     4081 gaaggtgata gagacattcg ctgtttttct ttggcgttcc gtcgggggcg atttgtttat
     4141 attctctgcc gttcatccac gcactgtccc tggcgcgtaa tattgttcct tttctcaggc
     4201 ctgttacagc cattaaaagc tccagtgtta cccattcatt cgcatttatc tgtacaacag
     4261 gtgtgatgac tggctgaatg ccatggttat gttggtttac taactcgcgc ataagttgcc
     4321 tctgcatgtg taagaaaaaa ccgcccgaag gcggttgtca gttgattgat gtacggcgca
     4381 tttttcgaag gctggcaata tgcttttcct tctcaatttc cgctttaatc atatgcagtt
     4441 cgttgtgctc gattcgctca aattcttcat taaatgcact aattgaagtg gctctggttc
     4501 tgccgtcgag ccttcggtag atcacctgag tcagagttac cttgcatatt tctaccggat
     4561 agttgttggc atcaacgaaa gactgcccgc gctggatcag gacgaacact ggttgtattc
     4621 cttgagtgcc atatcataaa cacggcatgc cgagaggtgt gttgtagtca acgtcgttcg
     4681 tatcctgaaa agtccatgtg ttgtgacggt aacttccggg gctttgagtt tcactgcatc
     4741 aacgatatgc tgatgtttgc ggctttcgaa tatagagctg gatataacca cacttgctgt
     4801 taaaccgtaa ctttcaaatg tgattttcat gtcattatcc tcgcagttag ccgggcaaca
     4861 aaaataaata atgttgttcc tgcgaataac aggataccgc ccgtcattag agaaaggaaa
     4921 aacaatatta atgtacgcgg gttgtttctc aaaatattct ctctgtgatt cgctcactct
     4981 gacaattcgt tgatcaagtc acggtattta ttcagttctt gcaacgcctt acatgcaccc
     5041 tcccatcgtt tctgtttccg cccggcgcgg cgcgcttctt tccgtgattt acgtagcagg
     5101 tcacctataa tgttgtgctg cactggctgc actggctgca ctggggcgat atttggtttt
     5161 ttgtccactt cccttgcaac tgcctgccag atgccatttt ttacagttac taccccctgg
     5221 ttttttaact tccatagagt gtcaataacg tcattcaaat caatattgag acctttagaa
     5281 atactttcgg ctgatgcttt tcctaatttg ttcaattctg tgagcactgc gttcattttt
     5341 ttctcctgat attttaatgt cggtggggct gttatacggt tagtgaggtg tcagcttctt
     5401 ctaccagctt ttccagttca tccagctttc gggaaagaat ttcaccaaac agatgaagtt
     5461 ctgcatctgc tgtaatcggg attggaacaa atcgtattcc acttctggca agttgatttg
     5521 cgatttccag acactgcctt aattcaactg gtgatgcctt tgtcaacgtc gttttttcgc
     5581 tcattggttt tctccagaaa tttaacaata cccggaacca tctcaatgga tggtgtgccg
     5641 cattggttac cccatacatc aaatccatgc gaggtgtggc gggcgaacag ttctatccgg
     5701 ggaacatcgc caagaagttg cacaagtttt tctcgagcca tatccggctt gcgggagtga
     5761 tcaaggcgtg gtgcagtgaa tgactggatt attccggcgt taatgcgttc gggcagattt
     5821 cctttcactg caaaaaggca gtcttcgctg ttagcgcgag tggtgctacc catacccatg
     5881 accagcttgt cggtctgtct tttcccgcat ttgttccagg ttaatccttt catcgtcacc
     5941 agacgaaagc cccacgcttc tacaaccttc aatgcttcaa gtggttgtgt aggcacccac
     6001 cacatagcca acaggcaatt atcggctgcc agttcccata ccgggaggcg gcagatatcc
     6061 agaagactca tgaccggata tttaaaactt gcaccgcgtt gaccatcggt tgctttgtcg
     6121 cggaatgtcc aggggggatc tgcgtaaatg agggtgtatt ttttcgtcat agcgcgtttt
     6181 caaactcatc aatgtaaagt cctgctttaa tcagtcggcg acggcgttcg gccttttgca
     6241 cgtattcctc gcgacagttt tttgctgcgt gttcacggct tctgcgactg aatggaggaa
     6301 cggcattgcg tgagcgaggt gtgtcacgac gaggatttga ttcgagggag aaaaagcgat
     6361 caacgattcc ggtgtctgtg gtgaatggtt ctgaagcctt tattttcaca accttactgc
     6421 cctttgtcag gccgcgagct accttgttga actcgcaaag tgaaacacca aatttctctg
     6481 ctatttcact gccagtaaca ggacgaccac gagactgaat catccaggtg acgcgttctt
     6541 ttaacccacg gaagacaccg ggccttccag aacgcctgta gaaagcgatc tgtttcaaat
     6601 ttttgtctcc agatatgaaa accccgacgg gatgtcgggg atggtagggg aagttacagg
     6661 cttaacgaga agccttttcg ggaatttcga tctgacagtc ttgaaatatc ctgtagcgtg
     6721 cggcgcattt cttccgtcag atgtttgaag gcgttaaatt cggcgacagc ggcgtcaacg
     6781 ttatagccct tgctgtgtag ctcgttgagg atgcgcatag ttgggcttgg catatcgaag
     6841 agcacagaaa cgtccaggtt aagagttttt cgatcaacat agctcatcat ggagtagggg
     6901 tgatgctctg aaaaccacga gagagggtag ttgatgttca ttggtgcagg taaaaggctc
     6961 tggcgaaact ttttctcgca ctcgatgaag tagcggcgga tctggcgacc tttttcgtta
     7021 cgttcaacca tcgcaagctc tttggctgtg tccagggtga ggtggtagtc cttgcggttg
     7081 tgaccgcctc taccttttgc ttcccgaatt tgggaagcaa tcatatagtc ctgattttca
     7141 acgaaaccat attcagcaat acgttctgtt atccacgatg caaagcgttt accaacccca
     7201 aggaaagcgt gtagattgcg agcattgcat agaagggctg cttggtttga gatagaaccg
     7261 ttgaatacgg ggatgagttg actagtcatt tttatgtcct tgctgatttg ttcagataac
     7321 cctgtgttca gcagggtggt caggtacttg aacaccgcag caagtcggcc cgtatcctta
     7381 gccttacggc tgtttttcgg tatacgcgct acccgaccat atctgaaaaa tggacacaaa
     7441 aaaaaccgca tgactgacgg ggcggtttcc gcttgctgtg gtgtgttcag caccatgaag
     7501 aggagtatcc gacagaatgt gtggcgtgtc aacatttcct tgtgtagatg ttgacaccct
     7561 attaggtaaa acattaacgt tgttttaagt tgttaataat tatcaattta atattaatgt
     7621 ttggagtata aaagcatgaa aaaatt
//
LOCUS       DAZKFU020000223         7605 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZKFU020000223 DAZKFU020000000
VERSION     DAZKFU020000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.225"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..262
                     /locus_tag="ACHOQQ_RS19440"
                     /old_locus_tag="pgaptmp_003888"
     CDS             <1..262
                     /locus_tag="ACHOQQ_RS19440"
                     /old_locus_tag="pgaptmp_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4472090.1"
                     /translation="NIGFNATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQD
                     INLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQKRSKK"
     gene            306..3522
                     /locus_tag="ACHOQQ_RS19445"
                     /old_locus_tag="pgaptmp_003889"
                     /pseudo
     CDS             306..3522
                     /locus_tag="ACHOQQ_RS19445"
                     /old_locus_tag="pgaptmp_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            3949..5604
                     /gene="lldP"
                     /locus_tag="ACHOQQ_RS19450"
                     /old_locus_tag="pgaptmp_003890"
     CDS             3949..5604
                     /gene="lldP"
                     /locus_tag="ACHOQQ_RS19450"
                     /old_locus_tag="pgaptmp_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGU4472091.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSVVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            5604..6380
                     /gene="lldR"
                     /locus_tag="ACHOQQ_RS19455"
                     /old_locus_tag="pgaptmp_003891"
     CDS             5604..6380
                     /gene="lldR"
                     /locus_tag="ACHOQQ_RS19455"
                     /old_locus_tag="pgaptmp_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGU4472092.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            6377..7567
                     /gene="lldD"
                     /locus_tag="ACHOQQ_RS19460"
                     /old_locus_tag="pgaptmp_003892"
     CDS             6377..7567
                     /gene="lldD"
                     /locus_tag="ACHOQQ_RS19460"
                     /old_locus_tag="pgaptmp_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGU4472093.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         1798 a         1883 c         2091 g         1833 t
ORIGIN      
        1 aaacattggt tttaatgcga catggggtgg tgaaaaacct gtcgatgcca aagagtttgc
       61 ccggtggcaa caattacttt tcaacacatc aatgaaatcc acattgaaat tattaccagg
      121 tcaatggcaa gacattaatt tgacactgaa gggtgtatcg ccgaataacc tgggatatct
      181 gaagttagcc atcaatatgg agaatattca gtttgacaac cttccctctg ctgaaaatcg
      241 gcagaaaaga agcaaaaaat aaatgcagct tctgcgctta tatcaaggaa tagagaacat
      301 cattaatgaa caaaatattt aaagttatct ggaaccctgc gacagggaat tatactgtta
      361 ccagcgaaac ggcaaaaagc cgtggcaaga aatccgggcg cagtaagctg ttaatttctg
      421 ccgtggttgc gggtgggttg ttgtcgtcgt ttgaggcact ggcaacaggt aatgaacctg
      481 gtacacctgt tggcacagga tctggctggg ttgctatagg ccaggaatca caggcaaatg
      541 cttttaccga tggtaatggt gccagtactg cagtgggtta taaagctttc gctgaaggaa
      601 catggagttc cgctattggc gccatgaccc atgcaattgg tccggcttca atggcattcg
      661 gggttaatgc cgtctcaacc ggtcaacgaa gtatcgctat gggcgcatcg tcaagttcag
      721 tggtcaatat tcgatggcac ttgggcgata tgcgaattcg ggtggtgatt attcgatcgc
      781 gcaaggggat aaatccaatg ctgctggtct gaatgccatt gcagtgggaa gaagcagtag
      841 tgcggcaggc gagaaagcca ctgcgctggg caacgcagct aaagctactg agattatgag
      901 tattgctctt ggcgacagcg ccaatgcgtc aaaagcgtat tcaatggcgc tggtagcaag
      961 tagcgtcgca tctgaagaaa acgcaattgc cctggggcgt agcagttagc tagcggtact
     1021 gacagcctcg cattaggcag acaaacactt gccagtacag cgaacgctat tgcgataggt
     1081 gctgagagca aagccgctga aaatgcgacg gccgttggta ccaaagcgga ggccaatgga
     1141 cttaatagta tcgcgttggg ctctgggggt attgcagatg ccgataacac gattgctctg
     1201 ggcaatcaaa gcctggcagt agctgctggt gcaattgcca tcggtcaggg gaataaagct
     1261 gacggcgcaa atgctatcgc gctgggtaat ggtagtgtta caggtggcgc aaatgctatt
     1321 gctcttggac aaggtagtta tgccggttta gaaaatggca ttgcaattgg tggtcaagcc
     1381 agtgctcaag ggacaaattc cgttgctctg ggtgcgggtt ctgtagcgac tgaagataat
     1441 tccgtctctg tgggtaacac aacagatcag cgaaaaattg tcaatatggc agcaggtgat
     1501 atcagcacta gcagtactga tgccataaat ggatcacagc tttatgctat cagtaagtca
     1561 gtagcggacc gtcttggtgg gggggctacc gtcaatgcgc aaggtgtcgt tactgccccg
     1621 aattacagac tgaaaaatgg tatttatggc aatgttggcg atgctttagc ggatctaaac
     1681 aataacacga tacaatggga ctccgttaaa aagggatata gtgcggcaca tggtacagat
     1741 gccaccagca ccatcaccaa cgttaaagcc ggcactattt ctgctaccag tacggatgcg
     1801 gttaacggtt ctcagttgaa tgcgacgaat atgatgattg agcagaacag ccaaatcatc
     1861 aatcagcttg ctggtaacac cgacgcaacc tatatcgaag aaaatggcgc tggtatcaac
     1921 tacgttcgta ctaacgacaa cggtttagcc tttaacgatg caagcgcctc tggtgtcggc
     1981 gctacagctg taggttataa cgctgtcgcg tctggtgcca gcagcgtagc cattggtcag
     2041 aacagcaata gcaccgttga taccggtatt gcgctgggta gcagctccgt ttccagccgt
     2101 gtgattgcca aaggttctcg tgacactagc gtaacggaaa atggcgtggt gattggttat
     2161 gacaccactg acggcgaact gctaggtgca ttgtcaattg gtaatgacgg taaataccgc
     2221 caaatcatca acgtagctga tggttccgaa gcccatgacg ccgttacggt tcgtcaattg
     2281 cagaatgcga ttggtgcggt cgcaaccacg ccaaccaagt actttcacgc caactcaacg
     2341 gcagaagact cactggccgt tggtgaagac tcgctggcaa tgggtgcgaa aactgtcgtt
     2401 aatggtaatg caggtattgg tatcggtttg aacactctgg ttctggctga tgcgatcaac
     2461 ggcattgcta tcggcagtaa cgcacgcgca aaccatgcaa acagtattgc aatgggtaac
     2521 ggttctcaga ccacccgtgg tgcacagact ggctacaccg cctacaacat ggacgcaccg
     2581 cagaactctg taggtgagtt ttctgttggt agtgaagacg gtcaacgtca gatcaccaac
     2641 gtcgcagctg gttcagcgga taccgatgcg gtcaacgtag gtcagttgaa agtaacggat
     2701 gcgcaggttt cccagaatac ccagagcatt actaacctga acaatcaggt cactaatctg
     2761 gatactcgcg ttactaatat cgaaaacggt attggcgata tcgtcaccac cggtagcatc
     2821 aagtacttca agaccaatac cgatggtgta gatgccagcg cgcagggtaa agatagcgtc
     2881 gcgattggtt ctggttccat tgctgccgcg gataacagcg tcgcactggg tacagggtct
     2941 gtggcaaccg aagaaaatac gatctctgta ggctcatcta ctaatcaacg tcgtatcacc
     3001 aacgtagccg caggtaaaaa cgacaccgat gctgttaacg tggcacagtt gaagtcttct
     3061 gaggcgggcg gtgtacgtta cgacaccaac gctgatggtt ctgttgacta tagcaatatc
     3121 accctcggtg ggggcaacgg cggtacgact cgtatcagca acgtctccgc tggcgtcaac
     3181 aacaacgacg cggtgaacta cgcgcagttg aagcaaagcg tgcaggaaac gaagcaatac
     3241 accgatcagc ggatggttga gatggataac aaactgtcta aaactgaaag caagttgagc
     3301 ggtggtatcg cttctgcaat ggcaatgacc ggtctgccgc aggcttacac tccaggtgcc
     3361 agcatggcct ctattggtgg cggtacttac aacggtgaat cggctgttgc tttaggtgta
     3421 tcgatggtga gcgccaatgg tcgttgggtc tacaaattac aaggtagtac caatagccag
     3481 ggtgaatact ccgccgcact cggtgccggt attcagtggt aatgatccat taagttagtg
     3541 tgactaaggg caaacgccaa attgcctgat gcgctacgct tatcaggcct acagggtgat
     3601 tctgcacatt cctataggcc gggtaaggtg ttcacgccgc atctggcaag ataaggcgct
     3661 ccgggtcaac aatctaaggg caattctctg atgaggattg cccttttctt taccagacat
     3721 ctccccccac aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt
     3781 ccccaattaa aactcacatc aatgttgcca atacataaca tttagttaac tattcattgt
     3841 cattatccct acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat
     3901 gagcaacaga ctcattacac gatgtgcgtg gactccagga gacctgcaat gaatctctgg
     3961 caacaaaact acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc
     4021 atcctgtttt tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca
     4081 tggacggtgg caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac
     4141 gcgctggcct cggtggttta tggcttcttc tacgggttgt ggcccatcgc gtggatcatt
     4201 attgcagcag tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc
     4261 tcgtctattc tttcgataac cccagaccag cgcctgcaaa tgctgatcgt cggtttctgt
     4321 ttcggcgctt tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca
     4381 ttgctggtcg gtctgggctt taaaccactg tacgccgccg ggctgtgcct gattgttaac
     4441 accgcgccag tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaggtaaca
     4501 ggtatcgaca gctttgagat tggtcagatg gtggggcggc agctaccgtt tatgaccatt
     4561 atcgtgctgt tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg
     4621 cctgcggtcg tggttgcggg cggctcgttt gccatcgctc agtaccttag ctctaacttc
     4681 attgggccgg agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg
     4741 ttcctcaaac gctggcagcc agtgcgcgta ttccgttttg gtgatttggg ggcgtcacag
     4801 gttgatatga cgctggccca caccggttac actgcgggtc aggtgttacg tgcctggaca
     4861 ccgttcctgt tcctgaccgc taccgtaaca ctgtggagta tcccgccgtt taaagccctg
     4921 ttcgcatcgg gtggcgcgct gtatgagtgg gtgatcaata ttccggtgcc gtacctcgat
     4981 aaactggttg cccgtatgcc gccagtggtc agcgaggcta cagcctatgc cgccgtgttt
     5041 aagttcgact ggttctctgc caccggtacc gccattctgt ttgctgcgct gctctcggtt
     5101 gtctggctga agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa
     5161 ctggctctgc ccatctactc catcggtatg gtgctggcat tcgcctttat ttcgaactat
     5221 tccggactgt catcaacact ggcgctggca ctggcgcaca ccggtcatgc attcaccttc
     5281 ttctcgccgt tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac
     5341 gccctgttcg ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg
     5401 ctggttgccg ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc
     5461 gctatcgcct gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact
     5521 gtcaaacaca gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat
     5581 gtcttaacgt ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg
     5641 ccgatcgtgt gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg
     5701 ctgaacgtca actggcgatg caactcggcg tatcacgtaa ttcactgcgc gaggcgctgg
     5761 caaaactggt gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct
     5821 ggcgtcatga cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg
     5881 atgatccgga ttacagtttc gatattctgg aagcacgcta cgccattgaa gccagcaccg
     5941 catggcatgc ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg
     6001 aagcaacgct aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg
     6061 cgattgccga agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg
     6121 tcctgcaatc ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt
     6181 cacaactgac cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg
     6241 gggcgcgtaa agcaatgatg gcgcacctta gttttgttca caccacaatg aaacgattcg
     6301 atgaagatca ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt
     6361 cgagggagaa aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg
     6421 cattctgccg ccgttcctgt tccactatat ggatgggggt gcatattctg aatacacgct
     6481 gcgccgcaac gtggaagatt tgtcagaagt ggcgctgcgc cagcgtattc tgaaaaacat
     6541 gtccgactta agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtggcgct
     6601 aggtccggta ggtttgtgtg gcatgtatgc gcgacgcggc gaagttcagg cagccaaagc
     6661 ggcggatgcg catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga
     6721 agtcgcgcca gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg
     6781 ctttatgcgt aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac
     6841 cgtggatatg ccgacaccgg gagcgcgtta tcgtgatgcg cattctggaa tgagcggccc
     6901 gaacgcggca atgcgccgct atttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt
     6961 gggcctgaac ggtcgtccgc atgatttagg aaatatttcg gcttacctcg gcaaaccgac
     7021 cggactggaa gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa
     7081 agaccttgag tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga
     7141 tccggaagat gcgcgcgatg cagtacgttt tggtgctgat gggattgtag tttctaacca
     7201 cggtggccgc cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga
     7261 tgcggtgaaa ggtgatatag ccattctggc ggatagcgga attcgtaacg ggcttgatgt
     7321 cgtgcgtatg attgcgctcg gtgccgacac cgtactgctg ggtcgtgctt tcctgtatgc
     7381 gctggcaaca gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat
     7441 gaaagtggcg atgacgctga ctggcgcgaa atcgatcagc gaaattacgc aagattcgct
     7501 ggtgcagggg ctgggtaaag agttgcctgc ggcactggct cccatggcga aagggaatgc
     7561 ggcatagtcg tttgcgcccc ctccccctaa ccctctccct caggg
//
LOCUS       DAZKFU020000224         7598 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZKFU020000224 DAZKFU020000000
VERSION     DAZKFU020000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.226"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1719
                     /gene="adeD"
                     /locus_tag="ACHOQQ_RS19465"
                     /old_locus_tag="pgaptmp_003893"
     CDS             <1..1719
                     /gene="adeD"
                     /locus_tag="ACHOQQ_RS19465"
                     /old_locus_tag="pgaptmp_003893"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGU4472094.1"
                     /translation="YQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIA
                     GVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICD
                     PHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRD
                     HPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAGIENC
                     HESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIA
                     HEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDAR
                     KVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRY
                     RVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNEGA
                     LAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMST
                     DTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFT
                     TLEVTE"
     gene            complement(1767..3158)
                     /gene="uhpT"
                     /locus_tag="ACHOQQ_RS19470"
                     /old_locus_tag="pgaptmp_003894"
     CDS             complement(1767..3158)
                     /gene="uhpT"
                     /locus_tag="ACHOQQ_RS19470"
                     /old_locus_tag="pgaptmp_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGU4472095.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3296..4615)
                     /gene="uhpC"
                     /locus_tag="ACHOQQ_RS19475"
                     /old_locus_tag="pgaptmp_003895"
     CDS             complement(3296..4615)
                     /gene="uhpC"
                     /locus_tag="ACHOQQ_RS19475"
                     /old_locus_tag="pgaptmp_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU4472096.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(4625..6127)
                     /gene="uhpB"
                     /locus_tag="ACHOQQ_RS19480"
                     /old_locus_tag="pgaptmp_003896"
     CDS             complement(4625..6127)
                     /gene="uhpB"
                     /locus_tag="ACHOQQ_RS19480"
                     /old_locus_tag="pgaptmp_003896"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGU4472097.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6127..6717)
                     /gene="uhpA"
                     /locus_tag="ACHOQQ_RS19485"
                     /old_locus_tag="pgaptmp_003897"
     CDS             complement(6127..6717)
                     /gene="uhpA"
                     /locus_tag="ACHOQQ_RS19485"
                     /old_locus_tag="pgaptmp_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGU4472098.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6879..>7598)
                     /locus_tag="ACHOQQ_RS19490"
                     /old_locus_tag="pgaptmp_003898"
     CDS             complement(6879..>7598)
                     /locus_tag="ACHOQQ_RS19490"
                     /old_locus_tag="pgaptmp_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472099.1"
                     /translation="SLLADTERQVLEGSSFVLCCGEKINGTISELLRKKITDSTHSIK
                     SLTLSESPSYEVYEEIFKKAVFTPDECHLVRDSLVQDGIKPEKIAKIISCLSSRTGIG
                     NLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSLVALNGRSYHQHVDFSEYGFT
                     QTLCMLGAVVNNANDTFDVANYIYTTKGENIIDNDAEDDSPYVHSDESYRHKDDYWAR
                     DNDVRNLRRNFSGNITIATYK"
BASE COUNT         1942 a         2120 c         1938 g         1598 t
ORIGIN      
        1 taccaggaat tgttagccgt ttcccgtggc gacgctgttg ccgattatat tattgataat
       61 gtctctattc tcgacctgat caatggcgga gaaatttccg gcccaattgt gattaaagga
      121 cgttacattg ccggtgttgg cgcagaatac actgatgctc cggctttgca gcggattgat
      181 gcccgcggcg caacggcggt gccagggttt attgatgctc acctgcatat tgaatccagc
      241 atgatgacgc cggtcacctt tgaaaccgct actctgccgc gcggcctgac gaccgttatt
      301 tgcgaccctc atgaaatcgt caacgtgatg ggcgaagccg gattcgcctg gtttgcccgc
      361 tgtgccgaac aggcaaggca aaaccagtac ttacaggtca gctcttgcgt gcccgctctg
      421 gaaggctgcg atgttaacgg tgccagtttt acccttgaac agatgctcgc ctggcgggac
      481 catccgcagg ttaccggcct tgcagaaatg atggactacc ctggcgtaat tagcgggcag
      541 aatgcgctgc tcgataaact ggatgcattt cgccacctga cgctggacgg tcactgcccg
      601 ggtttgggtg gtaaagaact taacgcctat attgctgcgg gtattgaaaa ctgccacgaa
      661 agttatcagc tggaagaagg acggcggaaa ttacaactcg gcatgtcgtt gatgatccgc
      721 gaagggtccg ctgcccgcaa tctcaacgca ctggcaccgt tgatcaacga atttaacagc
      781 ccgcaatgca tgctctgtac cgatgaccgt aacccgtggg aaatcgctca tgaagggcat
      841 atcgatgcct taattcgccg cctgatcgaa caacacaatg tgccgctgca tgtggcatat
      901 cgcgtcgcca gctggtcgac ggcgcgccac tttggtctga atcacctcgg cttactggca
      961 cccggaaagc aggccgatat cgtcctgttg agcgatgcgc gtaaggtcac ggtgcagcag
     1021 gtactggtga aaggcgaacc gattgacgcg caaaccttac aggcggaaga gtcggcgaga
     1081 ctggcacaat ccgctccgcc gtatggcaat accattgccc gccagccagt ttccgccagc
     1141 gactttgccc tgcaattcac gcccggaaaa cgctatcggg tcattgacgt catccataac
     1201 gaattgatta cgcactcccg ctccagcgtc tacagcgaaa atggttttga tcgcgatgat
     1261 gtgtgcttta ttgccgtact tgagcgttac gggcaacgtc tggctccggc ctgtggtttg
     1321 ctcggcggct ttggcctgaa tgaaggcgcg ctggctgcga cggtcagcca tgacagccat
     1381 aatattgtgg tgatcggtcg cagtgccgaa gagatggcgc tggcggtcaa tcaggtgatt
     1441 caggatggcg gcgggttgtg cgtggtacgt aacggccagg tacaaagtca tctaccgttg
     1501 cccattgccg ggctgatgag caccgacacg gcgcagtcgc tggccgagca aattgacgcc
     1561 ttgaaagccg ccgcccgtga atgcggtccg ttacccgatg agccgtttat tcagatggcg
     1621 tttctttctc tgccagtgat ccccgcgcta aaactaacca gtcaggggct atttgatggc
     1681 gagaagtttg ccttcaccac gctggaagtc acggaataat aaaaaaaaag cccggcgtca
     1741 tgccgggcaa aagtcaccag ttacgtttat gccactgtca actgctgaat ttttttctcg
     1801 cggcggattt tgcgttcttc catcaccgcc actatcgcca tcaggcagat acaaccaatc
     1861 gcggcgatat caagtgcggc gaaggtgcct gcccagccgg taaggccgaa taccggcgtc
     1921 ccatcggcaa tcattcccag acctaacttg gcaaagctgt caccaatcag gtaagcaaag
     1981 gtgcctttaa taccatcggc agcgccaatc gcttttttag gtacaaagcc aacagcagcc
     2041 acaccaatca acaattgcgg gccaaagacc aggaaaccca acgcaaagag agaagccaga
     2101 tagatatatt cgttgctggc atgttgatac acaccgagcg tggcgataat cagcgccagc
     2161 gcgatgcagg ccaccaggcc acggcgaccg ttcgccaggt cagagagcca gccccacagc
     2221 agcgtaccga ccagcgcacc agcttcaaac agcgtaaagc cctgaatcgc caccgcttta
     2281 gagagtttca gttcctggaa cgcgtatacg gttgaccact ggtcgatacc aatacgtacc
     2341 acatagagga aaatgttggc gaagcacagc agccagatca ctttgttttt cagcacatac
     2401 tcaacaaaga tctgccactt ggtcatatcg gtagattctg tctctttgtc ctcttcgctg
     2461 atctcctcgc cgaacagttc ttcagctttg ccgaggccat aagattccgg ggagtcgctg
     2521 ccgtaacgca ggccgataaa accgacaatc agcgcgataa tcgacgggaa gataaacatg
     2581 ccgatgacat ggccatcgaa caggtaattt gcgccgaaca gcgccacacc tgctgcgcct
     2641 gcaccgccaa ggttgtgaga tatgttccag aaaccgagga aggtcccgcg cttacgacgc
     2701 ggcgtccatt tggtgatggt ggagtaactg cacgaaccgc cggtgctctg gaaaaagccg
     2761 cttaaggcgt agaaggcaat catcagaaac aggctaaccg agccgctgcc catactggca
     2821 ctgaagccca gcatacaaat ggcagacagg ataagcatga acggcaggaa ttgtttggtg
     2881 tttttgccgt cggcgtagta ggaaaccagc gttttaccca cgccataagt gatggagaaa
     2941 cccaggccga tcatccccaa ctgcgtcatg ctcaacccgt aggtcgaaat catatcgttc
     3001 tgcgcgatgt taaagttctt gcgaatcagg tacatcgtca ggtagccgat aaagaccacc
     3061 aggtaggatt gcatgaacgg tttgaaccac attttgcgcc gcacttcgag cggaaggtcc
     3121 agggtcggct tgcgaacctg gtttaagaaa gccagcatgg gttactcctg aaatgaatac
     3181 ctgcgtgagg catgcattgt aaaaatcagc agcaaatcag gcctgagata gcgtccaggc
     3241 aaaacctgga aaatttctta gttttgccgg atatgaagtg aaaaggtgag atgcatcacg
     3301 cttcgcgcgg tgcctgggcg ttcaaaaagg gcagtaacag cagtgcggaa atcccggcgg
     3361 cgatagagat aaccacaaaa aatccgctcc agtgccaggt atcgagtact ttcgccagtg
     3421 gccaaccagc aagcgacgcc cccagataag caaacaagcc gacaaacccc gtcgccgccc
     3481 ctgccgcctc tttgtgggaa cactctgccg ccgccatgcc gattaacatc tgcgggccaa
     3541 agacaaaaaa accaatagtg aagaagcagg ttgcctgcat cacgtagctg gcaaatggca
     3601 tcagccacag cgagccgact gaaagcaaaa ttccggcggc gaaaatcaaa ttcatcggcc
     3661 cacggttgcc gttaaacaat ttgtccgagc cccaaccggc taccagcgca ccgataaatc
     3721 cgcccagttc aaacatcgtc actgccgtat tcgccgtgac cagatcgacg cccagcgtct
     3781 cggacatata caaattgccc cagtcgttga ttgccgcccg gaccacatag accagcacat
     3841 agcaaaacga aagcagccag atatacggat tcagcaacac atacttggtg aggatctctt
     3901 tacgcgtcag ccctgcccct tcttgttgtt gagcaatctc cagcgcgtcg tgccgccatt
     3961 cacctaccgc cggtaaacct aacgcctgcg ggcgatcgcg cagccgccag cagagaaaaa
     4021 tccccacgac gatcgccata caaccagcaa tcatcatccc ggcacgccag ccgtaatgta
     4081 gtgcagccgc tgccatcaca atgggaatga gtgcgccgcc gacgttatgc gccgtgttcc
     4141 ataatgccca ccaaccgccg cgctcggtac gagaatacca ggcggttaac agacgcgcac
     4201 acaccggtga accccagccc tggaaaaagg cgttcagcac ccagagtacg gcaaacgccc
     4261 atagcgacgt cgagaagcca aacagaatgt tgataatgcc cgtggcgata agccctatcc
     4321 ccataaaata acgggcattt gagcgatcgc tgacaatgcc ggagacaaac ttcgacacgc
     4381 cgtaagtaat gtaaaacagg gtcgctaaca ggccgatatc gctacggctg agcacgccgt
     4441 tagcaaggat ttctggtacg gcggcgttaa aacttttccg cgtgaagtaa aacagcgcgt
     4501 aacccagcca gatggtcagc agaatatgcc gacgccagta gcgatagcgt gcatcaattt
     4561 catgtttgtc agtcattaat ggcgcatcgg caggcgcttt cagaaacggc aacatcatcg
     4621 aaccttagac ataacgttga ggtagagaaa cgctgacacg cgtgccgtgc agacaggaaa
     4681 tgtgtaatgt gccgcccagc gccgttacgc gctcgcgcat tccggtgagg ccaaaacctt
     4741 gttgcccgga acccggcggc aaaccgctgc catcgtcttc aatcaccagc atcaaccgtt
     4801 catcctgctg ccagccttgc agcgtaaccg cgctggcatc ggcatgtttc acaatgttgt
     4861 tcagcccttc ctggcagacg cgaaacagcg tcacgcgctg gttttcactt aacgctgact
     4921 catcgatacg ccattcgaga tggctgacaa tgccgcgccc ttccagctcc atttcccgca
     4981 tcagtgagcg gatggcctgc tccagggtga gatcatccag ctggcgcgga cgtaaccgcc
     5041 ctaacaaacg gcgcaccgca tcgtaaacac ccagcgacag ttgttcgatg agctgcccgc
     5101 tctgcttcac gctggcgtta tctgccgcca gtcgctggac aatgcccgcc tgagtacgaa
     5161 tggcagtgat ggtctgaccg atatcgtcgt gcagctcacg cgccacatca cggcgcacgc
     5221 tctcttcggt ttctaacaaa cgttcagcca gatgctgatt gcgcgccagt tccttttgta
     5281 gcgactggtt aagttcacgc aaccgctgga tgcctgcgcc tagtaacaac cctgtcagac
     5341 tttgcaccag caacgagagc aataaatcca ccggatgatc gcgccaggtt tgactggcga
     5401 tcagcgcgat ggcgttcatc aacgtcgcaa tcagcgctcc ttgccaacca tagtgccagg
     5461 ccagcgcaat aatcggcagc gccaggcaga atggcgtaaa gcgcgacagt tcgtccggca
     5521 atcccaactg gagccagaga ctaataacaa acagcagcaa gtaccagacc agatgccgcc
     5581 cgcgccagtt gatcggttga gaaactaacg acgggccgag cggcagccag gtgttattgg
     5641 cgagatagtg ccagaacacc agacatatcg gggccagcgt caggccgcca gttaaagtca
     5701 gtagtagcgc attccacgac tctttgccgt gccaaagcca gggcagcgac tgcaacaacg
     5761 ccgccgccgt taacgccgcc ccctgtaaca gcaaggtgcg ccagtcacgc tgatggcgat
     5821 agcgcgagat cagcgctacg ggcagtaacg tcagtaaact accgatcatc aataaggaaa
     5881 aatgggttaa cccgaccgcc tgcgttagcc agtaaatcag cagccattcc gcgcccagca
     5941 atacgggcca gtatccgcgc gggcattgca gcattagccc cagacgcaga ccaaacggaa
     6001 ataacagcac cgccatatca gggcgctcaa ccaggtgcag gctgatactc cacaggcaaa
     6061 accatgcggc agagaagata aaaaagcagg caataacggt aattaagcgg gagaacaacg
     6121 tcttcatcac cagccatcaa acatgcggcg cgccagttca acgtcgttac tgacgcccag
     6181 tttttccatc agattggcgc gatggacgtg taccgttttc ggtgacaagc ccagttcggc
     6241 ggcaatctct ttcaccgcca ttccttgcgc cagtttttcc gccacctggc gttcgcgttt
     6301 ggtcagcggg tcctggcgac cggatgccag tttaatggca atatccggcg tcagataaca
     6361 gccgcccgtg gcaaccgtat gcaccgccgc aatcagttcg tcagggctac agcgtttgga
     6421 gagaaagccg cgcgcccccg cgttaagcgc ctgctcaacc agcgccggac tgtcatgaac
     6481 ggagagcata attgtcgcca tacctttcgg cagctggctt agcagttcca gaccggagat
     6541 atcgggcatg gagatatcgc aaatacacac ctgcacaccc cgccccggca gccccgccag
     6601 cgcctcgcgc cccgaaccaa actcggcaac tacttgcaaa tcaggctcca gccccagcaa
     6661 ctgcgcaaag ccggagcgga cgataaggtg atcgtctata agggcaacgg tgatcatgat
     6721 tgggtcctgg cggataaaaa acgcgcatac cttaacgata agcgcgatgt tgttcaagtt
     6781 ggatatgggg tatgtattgg ctgaatgatg ttgggcttat cgtcaaaacg cttacctgaa
     6841 aggtaagcgt ttggtgcgtg ttattgagaa aaagcgaatt atttgtaggt tgcaatcgta
     6901 atattgcctg agaaatttct ccttagattg cggacatcat tatcccttgc ccaataatca
     6961 tctttgtgtc tataactttc atcggaatgt acatagggag aatcatcttc agcgtcgtta
     7021 tcgataatat tttcgccctt agtggtatag atataattag caacatcaaa ggtgtcattc
     7081 gcgttattta cgactgctcc taacatgcaa agggtttgtg tgaatccgta ttctgagaaa
     7141 tcgacatgct gatggtaact tctaccattc aaagcaacca aactcggaca aacatctatt
     7201 gtatcgccaa cagatgtagc ggcgcgacgc acatcaagtg cagcgttatc attagcaaca
     7261 tgaaatgctt gtgaacaggc ataaaacaaa tttccaatcc cggtacgact ggataaacaa
     7321 gaaataattt ttgctatttt ttctggcttt ataccatcct gcactaaaga atctctcacc
     7381 agatgacatt catcaggtgt gaaaacagct ttcttgaata tttcctcata aacttcataa
     7441 gaaggagatt cagagagagt gagagatttg atggaatgtg ttgaatctgt aatttttttg
     7501 cgaagtaatt cacttatagt gccgtttatt ttttcaccac aacataaaac aaatgaactt
     7561 ccctccagaa cttgtctttc tgtatctgca aggagtga
//
LOCUS       DAZKFU020000225         7479 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZKFU020000225 DAZKFU020000000
VERSION     DAZKFU020000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.227"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..129
                     /locus_tag="ACHOQQ_RS19495"
                     /old_locus_tag="pgaptmp_003899"
     CDS             <1..129
                     /locus_tag="ACHOQQ_RS19495"
                     /old_locus_tag="pgaptmp_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4472100.1"
                     /translation="EMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKENHSRYVPG"
     gene            93..1181
                     /gene="tssG"
                     /locus_tag="ACHOQQ_RS19500"
                     /old_locus_tag="pgaptmp_003900"
     CDS             93..1181
                     /gene="tssG"
                     /locus_tag="ACHOQQ_RS19500"
                     /old_locus_tag="pgaptmp_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGU4472101.1"
                     /translation="MERESQPVRTRLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDHPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIGNRSGLGAGNRIRLSQRAPLGKTTGEACSRLLMTLETADPNEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPPAEDGHYRFE"
     gene            1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHOQQ_RS19505"
                     /old_locus_tag="pgaptmp_003901"
     CDS             1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHOQQ_RS19505"
                     /old_locus_tag="pgaptmp_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU4472102.1"
                     /translation="MVTVIRWWAPMFIACGLLAGCGLTQTVTDGTISITKSIFYKKIK
                     TLHLDFTPRTAINADGAQTPLATMVRVYQLRDRKAVDAADYQTLLRNADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
     gene            1744..4266
                     /locus_tag="ACHOQQ_RS19510"
                     /old_locus_tag="pgaptmp_003902"
     CDS             1744..4266
                     /locus_tag="ACHOQQ_RS19510"
                     /old_locus_tag="pgaptmp_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGU4472103.1"
                     /translation="MDNRSAFFDGQTRYFLEINGSAVKPDVLRFRGREALSEPFRWDI
                     GFTSRNANIPPEEVLMKYASLRMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLAL
                     LEYTRQCAVFQNQSVPEVVEQVLRKHGLEGADFEFRLERAYPSREIITQWQETDLQFI
                     QRILSEVGIYWRTEMDDVRGLDTYIFADSQLHYQFDVQLPYSEPSGLFDGAAESVWDV
                     RTWHNVATGTVTTRDYNYRTATTPMNATVSVRNDAVTTGEHYRYAAPYREAGDDASPE
                     PETESGAFYARLHHERELNRSACIHLFSNAAHLSPGQVLEPQGDVITDLKEGIVLTLV
                     TYRGARDSRLHVSAWGMPYTERYCFRPAEIPRPVIPGTLPARIESRGKNDIYAHLDGQ
                     GRYRVKMDFDREGTEPGFGYLWLRMAKPCAGETLGWHMPLIDGTEVAIAYSNGDIDLP
                     YIAYALHDAEHPDLVNRDNHTRNILRTPANNKLRMEDRRNEEHIKLATEYGKTQLNSG
                     HLVDSQGQLRGKGTELRTDEWGTLRAGKGLFVAADAQAKAQGEALDMDAALKEIDRLN
                     QQLQQLENAAERAQALKADIDSQIQMFEQRLKPLNEALVFSAPEGMALTSGEDMQMSA
                     ARNVAVNAGGDISTGTMGNLTALAGEKLGLFARTGQLSLKSGEGPVEVQAQNGSMRLF
                     AEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKVEYGTTASYLRKVKRTMTAAAQ
                     SMSMAFPRMSTSHIYSAIYRLLDKHGNILVNIPYRLTTPSGQTVTGYSDNEGRSVPVY
                     TRQQEDVELHFFVKKPQPEETMWFVGETDTQQLETELRESLP"
     gene            4266..5555
                     /locus_tag="ACHOQQ_RS19515"
                     /old_locus_tag="pgaptmp_003903"
     CDS             4266..5555
                     /locus_tag="ACHOQQ_RS19515"
                     /old_locus_tag="pgaptmp_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HGU4472104.1"
                     /translation="MEMPDLPETPTSDIPTGANNQPWDCTTTGVEMVEKQSRLPTTEN
                     RQYLHIKVEYAMRFPQLSAQIRRKGKTYQMSLKQTVMSGLIRVDEIARNYCWYYKAEV
                     AFDMRTTPPRPFLSSTLLEKDGAGRRHTTNPFPVPGRGIYRRPDVIIVKNKEDRWPGL
                     AGPDTEGNMHSDNLARLVEVKFPGDRLYEDQYEEYMQIVGGIGHEDRMTILEIHDCRP
                     EEHQWVDQVINVTMKAWQTSGAKYWPLALYPPVFMPRPPSTPVAARIEPWTYAGKAVS
                     DLSEGVVRGVAEGWNALSKETQLVLNSAASWLNDSGEWVYRQGSEVWVWASQTGKSVK
                     SWTNTQIKAAWTEIQHATDMTLDMLKQVDWVQVLTSVAKTVGVILVAVGVGGLVVTLG
                     IPEAIIGAFLLIVRLAISAWGTLATILGTGTAVLAVG"
     gene            5568..6782
                     /locus_tag="ACHOQQ_RS19520"
                     /old_locus_tag="pgaptmp_003904"
     CDS             5568..6782
                     /locus_tag="ACHOQQ_RS19520"
                     /old_locus_tag="pgaptmp_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3396 domain-containing protein"
                     /protein_id="HGU4472105.1"
                     /translation="MELHDFELNYIEKWLPEASVKYKDGSPAVNPGLIITVFFKDGHT
                     PEVRRRMVECVDRFYGEFKPWLKKTITKNWVGITDNNYAKKRQEILDSTPEEIFSWYL
                     SSAEKDFLAPDYSILIMGKRIFHNDNNRSVIKLTLPLSLLKETDGKQRYEAWLMWLCD
                     TFSVESGYAGLSFALPYARERMFPCEFALAQRFSGVMVDSLGTLEGGGAVIGLKGACW
                     YTILGTPWLEKLGGAERVKHRLAKTPEISLLPYNNGVVLKAGELPPPLGEMKTEGLSP
                     LLVKVNQVIKPVRFNESRSLHFYSEYEGLQFDKESTMKWYRRFDEASALLDKEEMGKS
                     CAPERIKCWTDEIAPHAGQWAAMVNGTTEYIQTREGQKMPPFEDKYGKQHRACWKLLK
                     RDDNGSVYTLPE"
     gene            6808..7187
                     /locus_tag="ACHOQQ_RS19525"
                     /old_locus_tag="pgaptmp_003905"
                     /pseudo
     CDS             6808..7187
                     /locus_tag="ACHOQQ_RS19525"
                     /old_locus_tag="pgaptmp_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
     gene            7184..>7479
                     /locus_tag="ACHOQQ_RS19530"
                     /old_locus_tag="pgaptmp_003906"
     CDS             7184..>7479
                     /locus_tag="ACHOQQ_RS19530"
                     /old_locus_tag="pgaptmp_003906"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HGU4472106.1"
                     /translation="MNSVSFSLAAQHLICSTKVTLFALVDGLQYERYFGESLSVQQPA
                     AVPLFDTWPDSRIAFAGPWVMEMNSIMDFRERLCELEAALPSVSWMISSSSLTEL"
BASE COUNT         1763 a         1878 c         2241 g         1597 t
ORIGIN      
        1 gagatgctgc accgtttctt tgcgctctat gctgatgttc accttttcaa ccagctcaca
       61 ctggtactgc aacccacagg gaaacgactg agatggaaag agaatcacag ccggtacgta
      121 cccggctgac gcaggccctg gcccttaaca acgatatctg gcgggccagt ttttatcgtt
      181 tctgccagct gctggagcag gaaaatccgg acgggccaaa actgggcacg accagccatc
      241 cgggcgatga tccggtgcga ttcagaccct ggcccgggat gggattcccg gtcagcaccc
      301 tgaaagcggt ggagatcgac gaagatcatc cgacattacc cccaacaatc cgcacgacat
      361 ttctcggaat gtatggggtg gactcacctc tgccgacgtc ctggctggat gatattacgc
      421 agcgccgtga aggacatgag gcactcacct catttctgga tattttcagc catcgcatca
      481 ccacgcagta ctaccggatc tggcgaaaat atgcgtaccc ggccactttt gaggaggggg
      541 ggcgcgatgc cacatcgcag tgtctgctgg ggctggtcgg gctcggtata cccggcaccg
      601 ctgaacaggt ggcaacgccg gtttcccgtt tcctggcctt actgggtgcc atgcgattac
      661 ccacccgcaa tgcggaaggc atccgggctc tggtgagtct gctggcaccg gatacccgcg
      721 cacttatcac cgaacccgat ccggttaagg tccatatcgg taaccgcagt ggactgggtg
      781 ccggaaaccg catccgtctt tcgcagcggg cgccgctggg taaaacgaca ggggaagcat
      841 gcagccggct cctgatgaca ctggaaacag cagatccgaa cgaagcggaa ggctggttgc
      901 ccggtggtgt cctccatacc gatttgctgg tcctgctgcg tgtttatctc ggctaccgca
      961 gtgacgtccg gctgcgcctt actgtccccg tgcggttgtt gcctgagccc cgcctgggca
     1021 aaggcaggcc tgttcagctg ggccgtaccg gtttgctggg gctgaaagcg ggcaaactca
     1081 gcaatggccg ggagagtctg acggtcagtc tgggctgcta cgaagggctg aaatgtgctg
     1141 cgctaccgcc tgctgaagac ggacattatc gcttcgaata atcacctgtt ctctctcaat
     1201 aaaggaacgc ctgaatggta acagtaatcc gctggtgggc gccgatgttt attgcctgtg
     1261 gcctgctcgc aggctgcggt ctgacgcaga ccgtcacgga cggcacgatc agcatcacga
     1321 aatccatctt ctataaaaaa atcaaaacgc tgcatctgga ttttaccccg cgtacggcca
     1381 ttaacgctga tggtgcacag acaccactgg cgacgatggt gcgggtttac cagctcaggg
     1441 atcgtaaagc ggttgatgcc gccgattacc agacactgct gcgtaatgct gatacggtac
     1501 ttaaggacga cgtgctggcc tcaaaagaac tgctggtgat gcccggcggc agcgtgacgc
     1561 ttaatatgcc catggatgaa gatgcgcagt ttgtggctgt ggtgggactg tttaaccggc
     1621 cggatcagaa ggacaatcgc tggcgcctgg tactgacccg tgatgacctc aatccggata
     1681 agccccgcac cattgaactg ggtgatggct ggctcagcct tgtgccggta aaggagtgac
     1741 gtgatggata accggtcagc gttttttgac gggcagaccc gttattttct ggaaattaat
     1801 ggaagtgcag taaagcctga tgttttacgt tttcgtggca gggaagcact gagtgagccg
     1861 ttccgatggg acatcgggtt cacctcccgg aatgcgaaca ttcctccgga agaggtgctg
     1921 atgaaatatg cgtcgttgcg gatgcgtaac ggcaaaaatg tccatggcat tgtgacccgt
     1981 ctggaatggc tgtccacatc gaaagaccag tcccactacc ggcttacact cagttcccgt
     2041 ctggcactgc tggagtacac ccggcagtgc gccgtatttc agaaccagtc cgtccctgag
     2101 gtggtggagc aggtgctgcg caaacacggg ctggagggcg ctgattttga attcagactg
     2161 gaacgcgctt acccgtcgcg tgaaattatc acccagtggc aggaaacgga tttgcagttc
     2221 attcagcgca ttctgtctga ggtggggatt tactggcgca cggagatgga tgatgtgcgc
     2281 ggactggata cgtacatttt tgccgacagc cagcttcact atcagtttga tgtgcagttg
     2341 ccttacagcg agccctcggg gttgttcgac ggcgcagcag aatcggtctg ggatgtgcgg
     2401 acctggcaca acgtcgccac cggcactgtc accacgcgtg attacaacta ccggacagcc
     2461 accacgccga tgaatgcgac ggtcagcgtg cgtaatgatg cggtcaccac cggggagcat
     2521 taccgttacg cagcacctta tcgtgaagcg ggagacgatg ccagcccgga gccggaaacc
     2581 gaatctggtg cgttttacgc ccggcttcac cacgagcggg aactcaacag gtcggcctgc
     2641 attcacctgt tcagcaacgc cgcacacctc tctcccgggc aggtgctgga gccgcagggg
     2701 gatgtcatta cggacctgaa ggaagggatt gtcctcacac tggtgactta ccggggagcc
     2761 cgggattccc ggctgcatgt gtcagcatgg ggaatgcctt acaccgaacg ttactgtttc
     2821 cgtccggcag aaatcccgcg tccggtcatc ccgggtacgc ttccggcacg gattgagagt
     2881 cgcgggaaga acgatatcta tgcccatctg gacgggcagg ggcgttaccg ggtgaagatg
     2941 gactttgacc gggaggggac ggaacccgga ttcggctacc tgtggctgcg gatggcaaaa
     3001 ccctgtgccg gtgaaacgct gggctggcat atgccgctca ttgatggtac cgaagtggcg
     3061 attgcataca gcaatggcga tattgacctg ccgtacatcg cgtatgcact gcacgacgct
     3121 gagcatcctg acctcgtgaa ccgggacaat cacacccgga acatcctcag aacgcctgcg
     3181 aacaacaagc tgcggatgga agacagacgc aatgaggagc atatcaaact cgccacggag
     3241 tacggtaaga cacagcttaa cagcggacat ctggtggaca gccaggggca actgcggggt
     3301 aaaggcacag agctgcgtac cgatgagtgg ggaaccctga gagcggggaa ggggcttttt
     3361 gttgctgcgg atgcgcaggc gaaagcacag ggcgaggcac tggacatgga tgccgcgctg
     3421 aaggaaatcg acaggcttaa tcagcaactg cagcaactgg aaaatgccgc agaacgggca
     3481 caggcgctga aggctgatat tgacagtcag atacagatgt ttgagcagcg gctgaaaccg
     3541 ctcaacgaag cgctggtctt ctccgctccg gaaggaatgg cgctgaccag cggtgaagat
     3601 atgcagatga gtgcagcacg aaatgtggct gtcaacgccg gaggtgatat cagtaccgga
     3661 acaatgggca acctgaccgc gctggccgga gagaagctgg ggctgtttgc ccggaccggt
     3721 caacttagcc tgaaatccgg ggaagggccg gtagaggtac aggcgcaaaa tggcagcatg
     3781 cggttgtttg ctgagaagaa gctgacactg agctcagcga gtgatatctc ctttgccggg
     3841 aaaaaacgca tcacgctgat tggcggggga agttatctgc gacttgaggc cgggaaggtt
     3901 gagtatggta cgacggcgtc gtatctgcgc aaagtgaagc ggacgatgac ggcggctgcg
     3961 cagagtatgt cgatggcatt cccccgtatg agtacttcgc atatctactc ggctatctat
     4021 cggttgctgg acaaacacgg caatattctg gtcaatatcc cctatcgcct taccactcca
     4081 tctggtcaga cagtaacggg gtatagcgat aacgaaggcc gctccgtgcc ggtttatacc
     4141 cgccaacagg aggatgtgga actgcatttt tttgttaaaa aaccacaacc cgaagaaacg
     4201 atgtggtttg tgggggaaac cgatacgcag caactggaaa ccgaactcag ggagagcctc
     4261 ccataatgga aatgcccgat ttacccgaga caccgacatc tgatattccc accggggcga
     4321 acaaccagcc gtgggattgc accacgacgg gtgtcgaaat ggtcgaaaag cagtcgcgtc
     4381 tgccaaccac ggaaaaccgt cagtacctgc atataaaggt ggaatacgcc atgcgttttc
     4441 cgcaactgtc ggcacaaatc cgccggaaag gtaaaactta ccagatgagc cttaagcaga
     4501 ccgttatgag tggtcttatt cgcgtggatg aaatcgcccg caattactgc tggtattata
     4561 aggcggaggt ggcgtttgat atgcgcacca cgccaccacg cccttttctg agcagtacgc
     4621 tgctggaaaa agacggtgct ggccgtcgcc acacgaccaa tcctttcccg gtgcccggca
     4681 ggggcattta ccgccgcccg gacgtgatta ttgttaaaaa taaggaggac cgctggcccg
     4741 gactggcggg gccggatacc gaaggcaata tgcacagcga taacctggcc cgcctggtgg
     4801 aggtgaagtt tccgggagat aggctgtatg aagaccaata tgaagagtat atgcagattg
     4861 tcggtggaat tggtcatgag gatcgcatga ccattctgga gatccacgac tgtcgcccgg
     4921 aagagcacca gtgggttgat caggtgatca acgtgacgat gaaagcctgg cagaccagcg
     4981 gcgcgaaata ctggccgctg gctctctacc cgccggtctt tatgccccgt ccgccatcaa
     5041 cccccgttgc tgccagaata gagccctgga cttacgccgg taaagcagta tctgatctca
     5101 gtgagggtgt ggtacgtggt gtggctgaag gctggaatgc cctgtcaaaa gagacgcaac
     5161 tggtcctgaa ttcagcagcc agctggctta acgacagcgg tgagtgggtt taccggcagg
     5221 gaagtgaggt ctgggtctgg gccagccaaa ccggtaagtc ggtgaaatcc tggaccaaca
     5281 cccagattaa ggccgcatgg acagaaatac agcacgccac cgatatgacg cttgatatgc
     5341 tgaaacaggt ggactgggta caggttttaa ccagtgtcgc gaaaacggtc ggcgttattc
     5401 tggttgctgt gggtgtcggc ggactggtcg taacgctggg tatcccggaa gccattattg
     5461 gcgcgttttt actgattgtt cgtctggcaa taagcgcctg gggaacactg gcgacgattc
     5521 tcggtacggg tacggcggta ctggcagttg gttaaacagg agcaggtatg gaattgcatg
     5581 attttgagct gaactacatt gagaaatggc tgccggaagc ctccgtgaaa tacaaagacg
     5641 gcagtccggc agtcaatccc ggtctgatta tcaccgtctt ttttaaggac ggacatacgc
     5701 cagaagtccg ccgcaggatg gtggagtgcg tggatcgctt ctatggggag tttaaaccct
     5761 ggctgaaaaa aacgatcacc aaaaactggg ttggcatcac tgataataac tacgccaaaa
     5821 agcggcagga gatcctcgat tcaacgcctg aagagatttt ttcctggtat ctgagtagcg
     5881 cagagaagga ttttttagcc cccgattaca gtattttaat tatggggaaa agaatttttc
     5941 ataatgacaa taatcgttca gtcattaagt taacactccc gttgtcgctg ctgaaagaaa
     6001 ctgatggaaa gcagcgttat gaagcctggc tgatgtggct gtgtgacacc ttttcggtgg
     6061 aaagtggtta tgcagggttg tcgtttgcac ttccttatgc ccgtgagcga atgttcccct
     6121 gtgaattcgc cctggcgcag cgtttttccg gtgtgatggt ggattcactg ggaacgctgg
     6181 aaggcggtgg ggcagtcatt ggtctgaaag gtgcctgctg gtacaccatt ctcggtacgc
     6241 cgtggctgga aaaactgggg ggggcagaga gagtaaaaca tcgtttagct aaaacgcctg
     6301 aaatcagcct gctaccctat aacaatggtg tcgtacttaa agccggtgaa ctccccccgc
     6361 cactgggaga gatgaaaaca gaaggtttat cgccgctgct ggtgaaggtg aaccaggtca
     6421 ttaagccagt gcggtttaat gaatcaagat ctcttcattt ctactctgaa tatgaaggac
     6481 ttcaattcga caaagaatcc accatgaagt ggtacaggcg ctttgacgaa gccagcgccc
     6541 tgctcgataa agaagaaatg gggaaatctt gcgcccccga acgcattaaa tgctggaccg
     6601 atgaaatagc gccccatgcc ggacaatggg ccgcgatggt taatggcacc accgagtaca
     6661 tccagacccg cgaagggcag aaaatgcccc cgtttgagga taagtacggt aagcagcaca
     6721 gggcttgctg gaaactactg aaacgagatg acaatggtag cgtttataca ctacctgagt
     6781 aaggtcgtgt gctgaatgac cgcgcaggcc ggagagaggc tggggctttt tgcccggacc
     6841 ggacaactga gcgtcaagtc cggggagggg ccggtagagg ttcaggcgca aaacggcagc
     6901 atgcggttgt ttgctgagaa gaagctgaca ctgagctcag cgtgtgatat ctcttttgcc
     6961 gggaaaaaac gtatcacgct gattggcggg ggagttacct gcgacttgaa gccgggaagg
     7021 tcgagtatgg tacgacggca aagtatctgc gcaaagtgaa gcggacaatg gcggcggggg
     7081 ctgcatcaat accagtcagg tccattgtgg ggggaggtat ttgcctgagc tgtctgatga
     7141 aggcggcaat gaatggcgac acctttgttg taaggggtgg gtcatgaaca gcgtcagttt
     7201 tagccttgcg gcacaacacc tgatttgttc aacaaaagtg acattatttg ctctggtgga
     7261 tggtctgcaa tatgagcggt atttcggtga gagtctgtct gttcaacaac ctgccgccgt
     7321 gcccctgttc gatacctggc ctgattcgcg tattgcattt gcggggccat gggttatgga
     7381 gatgaacagc ataatggatt tcagagaaag gctgtgtgag cttgaagctg cgcttccgtc
     7441 agtttcctgg atgatatctt cttcctcgct tactgaact
//
LOCUS       DAZKFU020000226         7403 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZKFU020000226 DAZKFU020000000
VERSION     DAZKFU020000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.228"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(80..1231)
                     /gene="argE"
                     /locus_tag="ACHOQQ_RS19535"
                     /old_locus_tag="pgaptmp_003907"
     CDS             complement(80..1231)
                     /gene="argE"
                     /locus_tag="ACHOQQ_RS19535"
                     /old_locus_tag="pgaptmp_003907"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGU4472107.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     NLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHLSNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            1385..2389
                     /gene="argC"
                     /locus_tag="ACHOQQ_RS19540"
                     /old_locus_tag="pgaptmp_003908"
     CDS             1385..2389
                     /gene="argC"
                     /locus_tag="ACHOQQ_RS19540"
                     /old_locus_tag="pgaptmp_003908"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGU4472108.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWSGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAQTQSLI
                     "
     gene            2397..3173
                     /gene="argB"
                     /locus_tag="ACHOQQ_RS19545"
                     /old_locus_tag="pgaptmp_003909"
     CDS             2397..3173
                     /gene="argB"
                     /locus_tag="ACHOQQ_RS19545"
                     /old_locus_tag="pgaptmp_003909"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGU4472109.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            3234..4607
                     /gene="argH"
                     /locus_tag="ACHOQQ_RS19550"
                     /old_locus_tag="pgaptmp_003910"
     CDS             3234..4607
                     /gene="argH"
                     /locus_tag="ACHOQQ_RS19550"
                     /old_locus_tag="pgaptmp_003910"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGU4472110.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            4874..5791
                     /gene="oxyR"
                     /locus_tag="ACHOQQ_RS19555"
                     /old_locus_tag="pgaptmp_003911"
     CDS             4874..5791
                     /gene="oxyR"
                     /locus_tag="ACHOQQ_RS19555"
                     /old_locus_tag="pgaptmp_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGU4472111.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(5774..7174)
                     /gene="sthA"
                     /locus_tag="ACHOQQ_RS19560"
                     /old_locus_tag="pgaptmp_003912"
     CDS             complement(5774..7174)
                     /gene="sthA"
                     /locus_tag="ACHOQQ_RS19560"
                     /old_locus_tag="pgaptmp_003912"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGU4472112.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         1704 a         1825 c         2096 g         1778 t
ORIGIN      
        1 atcgcatccg acattattgc ggcactggag tttggcaaca gtgccggatg cggcgcaagc
       61 gccttattcg gcctacgttt taatgccagc aaaaatggtg aatcacctgg gtgatcagtt
      121 cgcgggtggg cttgataaac ctcgtctcca gatattcatc aggctgatgg gcttgattga
      181 ttgaacccgg ccccagaacc agcgtcgggc ataacgtttg aataaacggc gcttcggtac
      241 agtagttcac cacttcggtc tttgctccga gcaatttctc aactacttca accagttgat
      301 gatttggcgg gcattcatag ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac
      361 ccggccagcg ttcgctcacc ggagccaatg catcgttgag caaaccatta agttcattga
      421 gtgtcatgcc aggcagcgga cgaatatcca tatgcaactc acagcaagcg caaatacggt
      481 tagacgcgtc gccaccgtga atatgcccga ggttgagcgt agggtatggc acggtaaacg
      541 cttcgtagtg ataacgttct ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt
      601 cgtgcattag ttcgatagcg ttaactccgc gtgctggatc gctggagtgc cccgactgac
      661 cctgaatacg gatggcgtta gagagatgac ctttatgtgc gcgtaccggt tgtagtgacg
      721 tcggttcgcc aatgatggcg caatccgggc gcagggcggt agtttcggca aaataacgcg
      781 ctccggccat actggtttct tcatcagcag tcgccagaat gtagagcggt tttttcagtt
      841 tcgtgacgtc gacatcgcgt agcgcatcaa ggataaacgc aaaaaagcct ttcatgtcgg
      901 cggtgcctaa gccgtaaagc ttgccgtcat gctccgtcag tgtaaacgga tcgcgcgtcc
      961 agcgaccgtc atcaaatggc accgtatcgg tatgccccgc cagcaacaag ccgccagccc
     1021 cctgtccggt acttgccagc atattgaatt tgttgcgagt tcctggcaca ggctgcactt
     1081 ccacattgaa gcctaaattt ttaaaccagt ccgccagcag agtgattaaa tctgcattgc
     1141 tttgatcgag tgcctcttcc gtggcgctta ttgaaggtgt ggcaatcaga gcacggtaaa
     1201 tctcgataaa tggcggtaat ttgtttttca ttgttgacac acctctggtc atgatagtat
     1261 caatattcat gcagtattta tgaataaaaa tacactaacg ttgagcgtaa taaaacccac
     1321 cagccgtaag gtgaatgttt tacgtttaac ctggcaacca gacataagaa ggtgaatagc
     1381 cccgatgttg aatacgctga ttgtgggtgc cagcggctac gctggcgcag agctagtgac
     1441 ctatgtaaat cgccatccgc atatgaacat aaccgctttg actgtttcag cgcaaagcaa
     1501 tgatgcggga aagttaatct ccgatttgca tccgcagcta aaaggcatcg ttgatctgcc
     1561 gttgcagccg atgtcggata tcagcgagtt tagcccaggg gtggacgtag tgtttctcgc
     1621 caccgcccat gaagttagcc acgatttagc gccgcagttt cttgaagcgg gctgcgtggt
     1681 gttcgacctt tccggcgcgt ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg
     1741 ctttacccat caatacccgg aactgctgga acaggcagcc tacggtctgg cggagtggag
     1801 cggtaataaa ttaaaagaag cgaatttgat tgcggtgcca ggctgttatc cgacggcggc
     1861 acagctggcg ctgaaaccgt tgattgatgc cgatcttctt gacctcaatc agtggccggt
     1921 gatcaacgcc accagcggcg tgagcggtgc aggacgtaag gcagcgattt caaatagttt
     1981 ttgtgaagtt agcctgcaac cgtatggcgt ctttactcat cgccatcaac cagagatcgc
     2041 cacacacctc ggtgctgacg ttatcttcac cccgcatctg ggcaatttcc cacgcggcat
     2101 tctcgaaacc attacctgcc gcctgaaacc gggtgtgacc caggcgcaag tcgcgcaagt
     2161 gttacagcag gcgtatgccc ataaaccgct ggtgcggctg tatgacaaag gcgttccggc
     2221 gctgaaaaat gtcgttgggc tgccattttg cgatatcggg tttgccgttc agggcgagca
     2281 tttgattatt gtggcgaccg aagacaactt actgaaaggc gcggcggcac aagcggtaca
     2341 gtgcgccaat attcgtttcg gctatgccca aacgcagtct cttatttaag ggtgcaatga
     2401 tgaatccatt aattatcaaa ctgggtggcg tactgctgga tagtgaagag gcgctggagc
     2461 gtctgtttag cgcactggtg aattatcgtg agtcacatca gcgtccgctg gtgattgtcc
     2521 acggcggcgg ttgcgtggtg gatgagctga tgaaagggct gaatctgccg gtgaaaaaga
     2581 aaaatggcct gcgggtgacg cctgcggatc agatagacat tatcactgga gcactggcgg
     2641 gaacggcaaa taaaaccctg ctggcatggg cgaagaaaca tcagattgcg gccgtaggtt
     2701 tgtttctcgg tgacggcgac agcgtcaaag tgacccagct tgatgaagag ttaggccatg
     2761 ttggactggc gcagccaggt tcgcctaagc ttatcaacac cttgctggag aacggttatc
     2821 tgccggtggt cagctccatt ggcgtaacag acgaagggca actgatgaac gtcaatgccg
     2881 accaggcggc aacggcgctg gcggcaacgc tgggcgcgga tctgattttg ctctccgacg
     2941 tcagtggcat tctcgacggc aaagggcaac gcattgccga aatgaccgcc gcgaaagcag
     3001 aacaactgat tgagcagggc attattactg acggcatgat agtgaaagtg aacgcggcgc
     3061 tggatgctgc gcgcacgttg ggccgtccgg tagatatcgc ctcttggcgt catgcggagc
     3121 agcttccggc actgtttaac ggtatgccga tgggtacgcg gattttagct taagttttgt
     3181 tggccggagg cgcagttttc cggcattgaa tttcaaaata aggaaacaga gttatggcac
     3241 tttggggcgg gcgttttacc caggcagcag atcaacggtt caaacaattc aacgactcac
     3301 tgcgctttga ttaccgtctg gcggagcagg atattgttgg ctctgtggcc tggtccaaag
     3361 ccctggtcac ggtaggcgtg ttaaccgcag aagagcaggc tcaactggaa gaggcgctga
     3421 acgtgctgct ggaagatgtt cgcgccaggc cacaacaaat ccttgaaagc gacgccgaag
     3481 atatccatag ctgggtggaa ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc
     3541 tgcataccgg gcgtagtcgt aatgatcagg tagcgactga cctgaaactg tggtgcaaag
     3601 ataccgttag tgagttactg acggctaacc ggcagctgca atcggcgctg gtggaaaccg
     3661 cacaaaacaa tcaggacgcg gtcatgccag gttacactca cctgcaacgc gcccagccgg
     3721 tgacgttcgc gcactggtgc ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt
     3781 tgcaggatgc gcttaagcgt ctggatgtca gtccgttagg ctgtggcgcg ctggcgggaa
     3841 cggcttatga gatcgaccgt gaacagttag ctggctggct gggctttgct tcagcgaccc
     3901 gtaacagtct cgacagcgtt tctgaccgtg accacgtgct ggaactgctg tcggctgccg
     3961 ctatcggcat ggtgcatctg tcgcgttttg ctgaagatct gattttcttt aacaccggcg
     4021 aagcggggtt tgtggagctt tctgaccgcg tgacttccgg ttcatcatta atgccgcaga
     4081 agaaaaaccc ggatgcgctg gagctgattc gcggtaaatg cggtcgggtg cagggcgcgt
     4141 taaccggcat gatgatgacg ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg
     4201 aagacaaaga aggtctgttc gacgcgctcg atacctggct ggactgcctg catatggcgg
     4261 cgctggtgct ggacggcatc caggtgaaac gtccacgttg ccaggaagcg gctcagcagg
     4321 gttacgccaa cgccaccgaa ctggcggatt acctggtggc gaaaggcgta cctttccgcg
     4381 aggcgcacca tattgttggt gaagcggtgg tggaagccat tcgtcagggc aaaccgctgg
     4441 aagaactgcc gctcgacgag ttgcagaaat tcagcccggt gattggcgaa gatgtctatc
     4501 cgattttgtc gctgcaatcg tgcctcgaca agcgtgcggc aaaaggcggc gtctcaccgc
     4561 agcaggtggc gcaggcgatt gcttttgcaa gggcgcggtt ggggtaagaa catttatatg
     4621 tacaaatttg agcctggctt atcgccgggc ttttttatgg caaaaaaaag cggatcctgg
     4681 agatccgcaa aagttcacgt tggctttagt tattcgagtt gagaaactct cgaaacgggc
     4741 agtgacttca agggttaaaa gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa
     4801 tgcttgatag ggataatcgt tcattgctat tctacctatc gccatgaact atcgtggcga
     4861 tggaggatgg ataatgaata ttcgtgatct tgagtacctg gtggcattgg ctgaacaccg
     4921 ccattttcgg cgtgcggcag attcctgcca cgttagccag ccgacgctta gcgggcaaat
     4981 tcgtaagctg gaagatgagc tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt
     5041 gttcacccag gcgggaatgc tgctggtgga tcaggcgcgt accgtgctgc gtgaggtgaa
     5101 agtccttaaa gagatggcaa gccagcaggg cgagacgatg tccggaccgc tgcatattgg
     5161 tttgattccc acggttggac cgtacctgct accgcatatt atcccgatgc tgcaccagac
     5221 ctttccaaag ctggaaatgt atctgcatga agcacagacc caccagttac tggcgcaact
     5281 ggacagcggc aaactcgatt gcgtgatcct cgcgctggtg aaagagagcg aagcattcat
     5341 tgaagtgccg ttgtttgatg agccaatgtt gctggctatc tatgaagacc acccgtgggc
     5401 gaaccgcgaa tgcgtaccga tggccgatct ggctggggaa aaactgctga tgctggaaga
     5461 tggtcactgt ttgcgcgatc aggcaatggg tttctgtttt gaagccgggg cggatgaaga
     5521 tacacacttc cgcgcgacca gcctggaaac tctgcgcaac atggtggcgg caggtagcgg
     5581 gatcacttta ctgcctgcgc tggctgtgcc gccggagcgc aaacgcgatg gggttgttta
     5641 tctgccgtgt attaagccgg aacctcgccg cactattggc ctggtttatc gtcctggctc
     5701 accgctgcgc agccgctatg agcagctggc agaggccatc cgcgcaagaa tggatggcca
     5761 tttcgataaa gtgttaaaac aggcggttta agccatttag cgcagctacc cgataggctt
     5821 ccgccatcgt cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac
     5881 ctttctgttc cataatcgcc tggccgatat gaataatttc ggcagcgcgc tcgccaaagc
     5941 agtgaatgcc cagaatctct ttcgtttccc gatggaacaa aattttcagc gtgcccacgt
     6001 tcatgccgac gatttgtgcg cgtgccagat gtttgaactg ggcgcggccc acttcatacg
     6061 gtactttcat cgcggtcagc tgctgttcgg ttttgcccac agagctgatt tccgggatgg
     6121 tgtaaatacc ggtagggata tcttcaatca gatgtgcggt ggcttcacct ttcaccagcg
     6181 cctgcgcggc aatgcgcccc tggtcatagg ccgccgacgc caggctcgga taaccaatca
     6241 cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata catactgttg accttcagct
     6301 gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc cagcgaatca gtattaccgg
     6361 tgcgaccgtt ggcatagagc aggcagtcag ctttcagttt tttacccgac ttcagatgca
     6421 tgatcacacc gtcgtcacag ccttcgatct tctcgtactc ttcgttgtgg cgaatcacta
     6481 cgccactgtt ccagaagtga taggagagag aatctgacat ctcttgatcg agaaatgcca
     6541 gcaggcgatc gcgggtgttg atcagatcca cttttacatc cataccgcgg aagatcgacg
     6601 catattcaca gccgatcact ccagcaccat agataagtac atggcgcggt tcgtggtgca
     6661 tgctaagaat tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat
     6721 gatatggacg agagccgcag gcaataacaa atttttcagc ggttagtgtt tcaacgctgc
     6781 cgtccgggca atccagcgcc aacgtatgct cgtcaacaaa gcgagcgttc ccctgcaata
     6841 tttcacagtg attacgttcg taaaatccct gacgcatgcg cgtttgttga ttaatcacgt
     6901 tatcggcatg gttaaggata tcggcaaaag aagagcggag cagtcgggaa tggtcgctgt
     6961 atagtgggtt ttgattgaat tctataatgc ggctgacggc gtgacggaga gctttcgacg
     7021 ggatggtgcc ccagtgggtg caaccgccgc caacattttg ataacgctcg ataactgcga
     7081 cgcgcgcacc ttgcttaacc aggcccattg cagcgccttc gccgccgggg ccggaaccta
     7141 ttactatggc atcgtaatcg taggaatgtg gcatggtagg gcttacctgt tcttatacat
     7201 aaaagcaaca gaatggtaac attttatcgc gggtaagcca attgatcccc gtcattaatc
     7261 tggctatagc gtgagcggcc tttgctttgt ctgcctcctt cttaacccat ttattcacta
     7321 ttaatcgctt tttattatgg ctgatagttt tatcttgccg aagcgtgctt ttttgttatt
     7381 gtctttatga aatcttctta taa
//
LOCUS       DAZKFU020000227         7390 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZKFU020000227 DAZKFU020000000
VERSION     DAZKFU020000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.229"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..982
                     /locus_tag="ACHOQQ_RS19565"
                     /old_locus_tag="pgaptmp_003913"
     CDS             <1..982
                     /locus_tag="ACHOQQ_RS19565"
                     /old_locus_tag="pgaptmp_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGU4472113.1"
                     /translation="KNNFAITVFASHRNERNEILSVADYSLTEPEDPQKRLQFIQETI
                     QTYGIHHIHTGRNNQWFEEHRTAIESTGATLTTGATGIDWLTLADEKVTFAQFMEQKG
                     LPVVPSWRVNTLAELKTRLAAPPFTDSPVCVKPVTGIYGMGFWRFDDSALPMAVFNHP
                     EHRLVSPQLYIAAASASESFKPLVLMPYLPGPEFSVDILADKGEILAAVGRRKEGAIQ
                     YLVNQGSAWELACDCARVMKADGLVNVQTRNDVNGNPVLLETNMRPSGGVGYTLHSGV
                     NLPGLFAAFKLGLMSEDAVIQSARNTFSPVAVRSITDVIVYPESLSNLLN"
     gene            992..2134
                     /locus_tag="ACHOQQ_RS19570"
                     /old_locus_tag="pgaptmp_003914"
     CDS             992..2134
                     /locus_tag="ACHOQQ_RS19570"
                     /old_locus_tag="pgaptmp_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HGU4472114.1"
                     /translation="MSDTLEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPILFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSENALSRRSTLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGKWDIQGKQSWGRLGMLAPAADLGHEVSFRKGER
                     VLVLGTGEFVWEPFLLAERLEAAGAQAFYGATTRSPIAVGYAIKSAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTRLLKALAEVAPVVEIVTYE"
     gene            2127..2900
                     /locus_tag="ACHOQQ_RS19575"
                     /old_locus_tag="pgaptmp_003915"
     CDS             2127..2900
                     /locus_tag="ACHOQQ_RS19575"
                     /old_locus_tag="pgaptmp_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472115.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLTPYQEKLASMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFSTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            2902..3981
                     /locus_tag="ACHOQQ_RS19580"
                     /old_locus_tag="pgaptmp_003916"
     CDS             2902..3981
                     /locus_tag="ACHOQQ_RS19580"
                     /old_locus_tag="pgaptmp_003916"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HGU4472116.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVDQKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGTALDIIEERHGTSGIVFVDGWTGKG
                     AITGELVRALRDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLLSR
                     SVWSSEGLHGCMVCEHLSEFECSRMLVDTVAHFRKKLTPSSLAPLSWNTESTRVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIIVTEMGGTLGQYRAVTIIKKVF"
     gene            3981..4937
                     /locus_tag="ACHOQQ_RS19585"
                     /old_locus_tag="pgaptmp_003917"
     CDS             3981..4937
                     /locus_tag="ACHOQQ_RS19585"
                     /old_locus_tag="pgaptmp_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGU4472117.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHEKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMRSLGKNNWPLLFIRPRHAEMGKWLKEHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMGGTHLSMMPTLETENVFDVIQMRELATRLLEHPCHKRI
                     IALRIGGNDLMNVVSLRRPRALTLYDSPMGYVIKMLVSVFSPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPDQIEVIQNALMVTQGEYSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLHNEQSADGIRLLTITQHH"
     gene            4948..6043
                     /locus_tag="ACHOQQ_RS19590"
                     /old_locus_tag="pgaptmp_003918"
                     /pseudo
     CDS             4948..6043
                     /locus_tag="ACHOQQ_RS19590"
                     /old_locus_tag="pgaptmp_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6061..6528
                     /gene="terW"
                     /locus_tag="ACHOQQ_RS19595"
                     /old_locus_tag="pgaptmp_003919"
     CDS             6061..6528
                     /gene="terW"
                     /locus_tag="ACHOQQ_RS19595"
                     /old_locus_tag="pgaptmp_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HGU4472118.1"
                     /translation="MQLNTRQARIFKLANLLGSGKPVSATDIITYLDCSEPTLTRALK
                     ELRKSYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNADLKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSGSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            6853..7390
                     /locus_tag="ACHOQQ_RS19600"
                     /old_locus_tag="pgaptmp_003920"
                     /pseudo
     CDS             6853..7390
                     /locus_tag="ACHOQQ_RS19600"
                     /old_locus_tag="pgaptmp_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT         1835 a         1871 c         1902 g         1782 t
ORIGIN      
        1 aaaaaataat tttgccatta ccgtttttgc ctcccaccgt aacgaaagaa atgaaatcct
       61 ttccgttgcc gattattctt tgactgaacc tgaagatcct caaaaacgtc ttcagtttat
      121 tcaggaaacc attcagacct acggcatcca ccatattcat actggtcgta acaaccagtg
      181 gtttgaagaa caccgaaccg ccattgaatc gaccggtgcc acccttacta ccggtgcaac
      241 gggcatcgac tggttaactc tggctgacga aaaagttact tttgctcagt ttatggagca
      301 aaaggggctc ccggtcgtac catcctggcg ggtgaatacg ctggcagaat taaagacacg
      361 cctcgcggct ccgccgttca ctgacagtcc ggtatgcgtg aagccggtga cgggtatcta
      421 cggcatggga ttctggcgct ttgatgacag tgctttgcct atggccgtct ttaatcaccc
      481 cgaacatcgt ctggtcagcc cgcaactgta tattgctgca gcgtcggctt ctgagtcgtt
      541 taaacccctt gttttgatgc cgtacctgcc tggcccggaa ttttccgtcg atatcctcgc
      601 ggataagggc gaaatactcg cagccgtggg acgccgtaag gaaggggcta tccagtatct
      661 ggtaaaccaa ggaagcgcct gggaactggc gtgtgactgc gcccgggtta tgaaggccga
      721 cgggctggtg aatgttcaga cgcgaaacga tgtgaatggc aacccggtgc tgcttgaaac
      781 caacatgcgt ccgtcaggcg gggtgggtta tacccttcac agcggggtta accttcccgg
      841 gttgtttgct gcttttaagc tcggtctgat gtctgaagat gcggtgatcc agagcgccag
      901 aaataccttt tctccggttg cagtgagatc catcacagat gtcatcgttt acccggaatc
      961 actctctaac cttctgaatt aaggcgtgtt tatgtctgat accctcgaag atatgattta
     1021 tcgccgtacc ctctcatgcg gtacgattca ggtaacccgc gaccagggtg aagtttcgct
     1081 cgatgacctg tttgatatcg ctgaaagacg taacccgaaa cgcgcctttc tgtttgtcag
     1141 caaagtactc ggcaggcaca ttcccgtgcc accctcagtc atgcggcagg cctacagaca
     1201 gcttgccagc cagttcccct caacgctgac agggcccata ctgtttatcg gtatggcaga
     1261 aaccgccgtt gggcttgggg ctggtgtgtt tgatgaagtg cgccaccagc atcgtgaatc
     1321 tgtctatctg acctctaccc gtcacccggt tgatggcaca ttgctttgcg agttcaagga
     1381 agaacacagc cacgccaccg atcatctgat ctatctgcca gacgatgaag agaaaagacg
     1441 tcgtgttaca aacgcgcgaa ctctggtttt gattgatgac gaagcaacca ccggtaatac
     1501 ctttattaac ctgctttctg ccctgcgtaa taccggcaag cttcaacata ttgaacaggt
     1561 tatcgctgtt acgcttacgg actggagcga aaatgccctg tccaggcgca gcactttacc
     1621 cgtcacttcg gtttctcttg taagtggtca gtggggatgg actccattac cagatgcacc
     1681 tgtcccggac atgccgaagg tcaacgtaac ttcacgtgga aaatgggaca tccagggaaa
     1741 acagtcctgg ggtcgactgg ggatgctcgc acctgcggcc gatctcggtc atgaggtatc
     1801 cttccgcaag ggggagcgtg ttctggttct cgggacaggg gaattcgtct gggagccgtt
     1861 cctgcttgct gaacggctcg aagctgccgg agcacaggca ttttatggtg cgaccacccg
     1921 ctcccctatc gccgttggtt atgccattaa gtccgcaatt tcctttacgg ataactacgg
     1981 cctgggcatc cccaattttg tctataacgt cgcccatcag caatttgacc gcattcttgt
     2041 gtgtactgag acacctgcag aaagtattga cacgcggctt cttaaagcgc tggctgaggt
     2101 tgcacccgtc gtggagattg ttacctatga ataaacccgt tgttctgagc gaccttgacg
     2161 acacgctctt tcagacccgt cgtaaaatgg tagacgagct ggccctggag ccattccgta
     2221 ccggtgccgt tgatcgtact ctgaatccac gaagctttat gacagaagaa cagtccatgt
     2281 tagtggactg gctcctagag caggctgaac tcattcccgt taccgcacgg ggaactgaag
     2341 aaatcagccg cgtaaggatc cctttccact cctgggcaat caccacgcac ggggccgtca
     2401 ttctcacgcc tgaaggcaaa cccgacgagg agtggaaggc ccatatgctc ggccagctaa
     2461 ctccctacca ggaaaagctg gcatcgatgc agcgtctgat cactgaaatg atggacgcaa
     2521 aagggatcaa tgcctgggca agactgaact tcgaatacgg tgaaacggcg gtttatatgg
     2581 tgatgaaaca ccgcgacagc actcgccttg acgaactcaa tgccattgca gatgagatag
     2641 aaacggtatt ttcgaccgag ggcttctaca tccaccgcaa cagtaacaac gtagcctggc
     2701 ttcccacccc ggttgagaaa gggctggccg tcagctggct gcttgaaaaa cttcgggctg
     2761 aacgcggagt tttccccgta attggtctgg gcgacagcct gagcgatcat cgttttatga
     2821 aactgtgcag ctggtttggc atcccgcgtc agagtcagtt tgcagatgcc atttcacagc
     2881 gaatttttgg agaaaattaa aatgcagcca catgaaacat ttaccggctc ataccagccc
     2941 ggtgacgtgg aatttctgct aaagccggtt gtcattgaaa tgacgccagt tgatcaaaaa
     3001 gaagagctga ttcagtccgg gaagaaacat tattcggaca tgctcagcca ggagccagcg
     3061 ccgacacaat ggcatcttga tttgtttcac cgggcgctgg atcggggcgc agagagactt
     3121 gcaaaagaag tcacacagct cgctattgct ctggccgaac gcttcggtga tgagcccata
     3181 gtactggcca gtcttgtcag ggctggcgta ccgctcggcg ttatgctaca ccaggctctg
     3241 cgtgacatgg gaaaaacctc atggcattac ggcatcagca tcatccggga tcgtggaatt
     3301 gacggtacag cacttgatat cattgaagag cggcatggta ccagcggcat tgtctttgtt
     3361 gacggatgga caggtaaagg cgcaattacc ggagagcttg tacgcgcact gagagatcgc
     3421 ccgggctatc ctgagcagcc gcgactggtt gtcctggccg atccctgtgg ctgttcctgg
     3481 cttgcagcaa gtgatgatga ctggctcatt ccttttggca tcatgggggc tccggtgtca
     3541 ggcctgctct cccggtcagt atggtcctct gaaggattac atgggtgcat ggtttgcgag
     3601 catctcagtg aattcgaatg cagccggatg cttgtcgata ccgtcgctca tttccgtaag
     3661 aagttaacac cgtcatccct cgctcctctg agctggaata cggagtcaac ccgggtctta
     3721 tggcagacaa gtcgcgacgt tatcgcgttc ctggccgatg aatttaaagt ggacagcgtc
     3781 aaccgtatta aacccggtat tgctgaagca acccgggctg tattacgtcg ggtaccggac
     3841 catgtatttg tgcgttcgat tgacgacccg gacgttgcct tacttgtggg gcttgctcgt
     3901 gaaaagggaa taattgttac agaaatgggg ggaaccctcg gccagtatcg ggctgtaacc
     3961 attatcaaaa aggtattcta atgaaaaatc gtctttctcc ctggaatctt ggtgccactc
     4021 tatacatgcc tgcaacacgg gaagatattg ctgatgccgt tctgcacgag aaaatcccgg
     4081 gtttgcgctc tctggtgatt tgcctggagg atgctgtcag tgaagcagac atccccgtcg
     4141 ccctcaaaaa tctagagcac ctgctgcacg aactcagtaa cagcatgcgt agcctgggta
     4201 aaaataactg gcccctgttg ttcattcgcc cccggcatgc cgaaatgggc aaatggttaa
     4261 aagaacatta tgatctttcc gctgtcgacg ggtttgtgtt accgaaattt accctaagtt
     4321 cgttagctga atggtgggat atcatgggcg gaactcacct gagcatgatg ccaacgctgg
     4381 aaacagaaaa cgtctttgat gtgattcaga tgcgcgagct ggccacccgg ctgttggaac
     4441 atccctgcca caagcgcatt attgcgcttc gaatcggcgg caacgatctt atgaacgtcg
     4501 tgtcgcttcg ccgtccccga gccctgacgc tgtatgacag tccgatgggc tacgtcatca
     4561 aaatgctcgt ttcagtcttc agcccgcgtg attttgccct gaccgcgccc gtatgtgagc
     4621 atattgacga tcatgccgtt atggccagag agctggctct tgatatggca catggacttg
     4681 ttggaaaaac ggccattcat ccagatcaga tagaggtcat tcaaaacgcg ctgatggtca
     4741 ctcagggtga gtactctgac gctctgcgga tcctgaactc tacccaggcc gtgtttaagt
     4801 cgcagggggc gatgtgtgaa cctgccacac atcgccgctg ggcggctggc attctggaca
     4861 gagcccggtt ttacgggtta cataacgagc aaagcgctga tggaatcaga ttacttacta
     4921 taacccagca tcattaagga aatcataatg gggttcaggt tcagaggtac ttccgtttct
     4981 ttctgtagcc ggggggccta tgccaatctg gggttacccg gtaccgagct gagttaccgt
     5041 acccggtttg agctggccgc gccttccgga ggtggaaaca ggacagcaac cgtcccggat
     5101 ctcagacagt cgcttgagca ggaagcagtt gaactcatgt cagcggtgac ctcaattcgt
     5161 aacattcacg agttgacgct ggatccaaaa acaggcatca attgggcaga gctggaagca
     5221 gtatacctgc atagcagaac atcgcttttt caggttccgg caccagtgcg tcctgaaaaa
     5281 ccagaatacc atgcattgtc ggaaaatcct gcagggaaca aaggcattgt ttttctgggt
     5341 aaatggtttg aatcggaatc agctaaagct gagcgtcaag cagggaatct tcgccgatgg
     5401 cggcaagaac tgattgatgt gaagcgtgag aatccccttc gacagcaccg gtaccagcaa
     5461 cggcagacga ccagggccga acagtattca aactggaagt gtgatgctga agaacatgaa
     5521 aaaaggctcg ccacgactca ggcagatgcc cggcagttgt tccgggcaga cgcagcgttt
     5581 ttcgaatcat accttgcggg tgtgctttca gaaactgaat ggccgcgtga aacgcttgtt
     5641 gcatttgaag taaagccgga gatatcaaca gtcctgccgg atgttggttt agctgagatt
     5701 gaagatttcc ctgataagat ttacggcgtc aatgcccggg gcatggagct gacgcaaaaa
     5761 gccatacgcg aaaactatgc ccgccacagc catggctgct tgttccgact ggtcggtatc
     5821 gtgttacata cgctaccttt cgacaaggtg atcgcatcag gatttacaca acgagtaagc
     5881 ggaacgacta tctggaggat gtttatatcc tgtcctgcaa atgcactcgc agccagaagg
     5941 catagaacat attgatccgg ttgaagcgtt aggctatcac cctgttattc gaaagatgag
     6001 aaacacattc atttttcagc ctattgaacc actaaccctt taagcaaact aaatattacg
     6061 atgcaattaa ataccagaca ggccagaatt tttaagcttg ccaacttact gggctccggc
     6121 aagcctgttt ccgctaccga cattataacc tatctggact gctcagagcc gacattaaca
     6181 cgggccctca aagagctacg aaagtcatat tctgcagaaa tcaaatacag taaggcaggt
     6241 cattcctatc atctcgttaa tccgggtcaa ctggataaaa agacacttcg ccggatgaat
     6301 gaggcgcttg cacagaatgc tgatctgaaa accggtgagt ctacaggaaa ggtcgtactc
     6361 gataaggata aaaaaacagc cgtatcatta tcgctacgca tgcgaatttt aagaaaaatt
     6421 gaccgtctgg ctgcactgag tggatcgacc cgaagtgaag ctgttgaaaa actggcactg
     6481 cattccgttg acgaactgat aaaagaatac agcgctaaaa aatcctgatg acaaatctca
     6541 gctcggggtg aataccccgg ttttttcata tttcaactct gtcttggcgt gacgtatttt
     6601 tgatctattc tcctcccgca atgccagcag ctccttacag cctacaaaac ctagcgtctt
     6661 gacatcctct tgtgctacgc atcaatcttt gggcgattta cactccactg actgccgtat
     6721 gtagccatta agccagtatt ggtaacgtag atgcccacct tttcacttat ttggtggaca
     6781 ccactttgtc taactcgtca gattctgacc aaacgtttca ggctgtacgc ttactctgaa
     6841 ggggggagaa tagtagcccg tgatagcatt gagctaactg acagccagat cgcagcacta
     6901 aagcgtaaag ccagtgatga cgaggcctgt ggcgaaagcg aaacggctca tccgggatat
     6961 ctgggttcgc aggacacgtt ctatgtaggc aacctgaaag gtgtcgggcg tatttatcag
     7021 cagacgttcg ttgatacgta ctcgaaagtg gctcactgta agctttacgt caccaaaaca
     7081 ccgattaccg cggctgactt actgaatgat cgtgtactgc cgttttatac gtctcagggc
     7141 ctgccgatgc tgagaatact gaccgacaga ggcacggagt actgtggcaa agtagagcag
     7201 catgattatc agctttatct ggcaataaac gatatagaac acacgaaaac aaaggcgatg
     7261 tccccgcaga ccaacggcat ctgcgagcgg ttccataaaa caatcctgaa cgaattttat
     7321 caggtaacgt tccgtaaaaa actgtacggc gatcctgata cattacagtc ggatcttgat
     7381 gaatggctgg
//
LOCUS       DAZKFU020000228         7379 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZKFU020000228 DAZKFU020000000
VERSION     DAZKFU020000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.230"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..189
                     /gene="ydgT"
                     /locus_tag="ACHOQQ_RS19605"
                     /old_locus_tag="pgaptmp_003921"
     CDS             <1..189
                     /gene="ydgT"
                     /locus_tag="ACHOQQ_RS19605"
                     /old_locus_tag="pgaptmp_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGU4472119.1"
                     /translation="FRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSG
                     GRLFDLGQVPKSVWHYVQ"
     gene            275..715
                     /locus_tag="ACHOQQ_RS19610"
                     /old_locus_tag="pgaptmp_003922"
     CDS             275..715
                     /locus_tag="ACHOQQ_RS19610"
                     /old_locus_tag="pgaptmp_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGU4472120.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            792..1373
                     /gene="rsxA"
                     /locus_tag="ACHOQQ_RS19615"
                     /old_locus_tag="pgaptmp_003923"
     CDS             792..1373
                     /gene="rsxA"
                     /locus_tag="ACHOQQ_RS19615"
                     /old_locus_tag="pgaptmp_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGU4472121.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            1373..1951
                     /gene="rsxB"
                     /locus_tag="ACHOQQ_RS19620"
                     /old_locus_tag="pgaptmp_003924"
     CDS             1373..1951
                     /gene="rsxB"
                     /locus_tag="ACHOQQ_RS19620"
                     /old_locus_tag="pgaptmp_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGU4472122.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            1944..4262
                     /gene="rsxC"
                     /locus_tag="ACHOQQ_RS19625"
                     /old_locus_tag="pgaptmp_003925"
     CDS             1944..4262
                     /gene="rsxC"
                     /locus_tag="ACHOQQ_RS19625"
                     /old_locus_tag="pgaptmp_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGU4472123.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAISRA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEEPVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQPANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVP
                     EEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            4263..5321
                     /gene="rsxD"
                     /locus_tag="ACHOQQ_RS19630"
                     /old_locus_tag="pgaptmp_003926"
     CDS             4263..5321
                     /gene="rsxD"
                     /locus_tag="ACHOQQ_RS19630"
                     /old_locus_tag="pgaptmp_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGU4472124.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLTLCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            5325..5945
                     /gene="rsxG"
                     /locus_tag="ACHOQQ_RS19635"
                     /old_locus_tag="pgaptmp_003927"
     CDS             5325..5945
                     /gene="rsxG"
                     /locus_tag="ACHOQQ_RS19635"
                     /old_locus_tag="pgaptmp_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGU4472125.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            5949..6644
                     /gene="rsxE"
                     /locus_tag="ACHOQQ_RS19640"
                     /old_locus_tag="pgaptmp_003928"
     CDS             5949..6644
                     /gene="rsxE"
                     /locus_tag="ACHOQQ_RS19640"
                     /old_locus_tag="pgaptmp_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGU4472126.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADSLLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            6644..7279
                     /gene="nth"
                     /locus_tag="ACHOQQ_RS19645"
                     /old_locus_tag="pgaptmp_003929"
     CDS             6644..7279
                     /gene="nth"
                     /locus_tag="ACHOQQ_RS19645"
                     /old_locus_tag="pgaptmp_003929"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGU4472127.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT         1699 a         1910 c         1972 g         1798 t
ORIGIN      
        1 tttcgcaaaa tcagttcact cgaaagtctg gaaaaacttt acgaccatct taattacacc
       61 ctgacggacg atcaggaact gatcaatatg tatcgtgctg ccgatcaccg tcgcgcagag
      121 ctggtttctg gcgggcgttt gtttgacctc ggccaggtac cgaagtccgt ctggcactat
      181 gtccaataaa gaaagtagcg atcggttata aaagccttat aataacgccc ctaaaattag
      241 atttgcctgt ttgcgtcgct attctggaga acctatgacc accacaacgc cacaacgtat
      301 tggaggctgg ttgcttggcc cgttggcctg gcttttagtc gctctgttaa gtacgacgct
      361 ggcgttgttg ctgtataccg ctgcgttatc ttctccgcaa acatttcaaa cgctcggcgc
      421 acaagcactg acgacacaaa tcttatgggg cgtttctttt attactgcta tcgccatgtg
      481 gtattacaca ctgtggttga ccatcgcttt ctttaaacgt cggcgttgtg ttcctaaaca
      541 ctatattatc tggctgctta tttccgtatt gctggcagta aaagcgttcg ctttttcacc
      601 cgtggaagat ggcatagccg ttcgccagtt gctgtttact ttgttggcaa ccgcattgat
      661 cgtcccctat ttcaaacgct cgtcgcgggt gaaagcgacg tttgtgaatc cgtaataacc
      721 ttacagttaa cctgttgtcg cctgctctgg attaacggat aataggcggc ttttttattt
      781 caggccgaaa aatgactgac tacctgttac tgtttgttgg aactgtactg gtcaataact
      841 ttgtactggt caagtttctc ggtctctgtc cgtttatggg agtttccaaa aagctggaaa
      901 ccgcgatggg catggggctg gcaacaacgt ttgtgatgac gctggcgtct atttgcgcct
      961 ggcttatcga tacgtggatt ttgatcccac ttaatctgat ttacctgcgc accctggcat
     1021 ttattctggt gattgctgtg gtcgtgcagt tcaccgagat ggtggtgcgc aaaaccagcc
     1081 cggtgcttta ccgtttgctg gggatttttt tgccgcttat caccaccaac tgtgccgtgc
     1141 tcggcgtggc gttgctgaat atcaatctcg ggcacaattt cttgcagtcg gcgctgtacg
     1201 gtttttccgc cgctgtcggt ttttcgctgg tgatggttct cttcgcagcc atccgcgaac
     1261 gccttgctgt ggctgatgtc ccggcacctt ttcgcggtaa tgccattgcg ttaattaccg
     1321 caggtcttat gtctctggcc tttatgggct ttagtggttt ggtgaagttg taatgaatgc
     1381 tatctggatt gccgttgccg ccgtgagcct gctgggcctg gcgtttggcg ccattctggg
     1441 ttatgcctcc cgccgttttg cggtggaaga cgatccggtc gttgagaaaa ttgacgaaat
     1501 cttaccgcag agccagtgtg gtcagtgcgg ttatcccggc tgtcgcccct acgcggaagc
     1561 catcagctgt aacggtgaaa aaatcaaccg ttgcgcccca ggtggcgaag ctgtgatgct
     1621 aaaaattgcc gagttgctta atgtcgagcc gcagccgctg gatggcgaag cgcaagagct
     1681 aacgcctgcg cggatggtgg cggttattga tgaaaataac tgtattggct gcactaaatg
     1741 tattcaggcg tgtccggtag acgccatcgt tggcgctacc cgtgccatgc atacggtaat
     1801 gagtgatctc tgtacgggct gcaatttatg tgttgatccg tgcccgacgc actgcatctc
     1861 gttgcaaccg gtcgcagaaa cacctgactc ctggaaatgg gatctgaaca ccattcccgt
     1921 gcgtatcatt cccgtggaac accatgctta agttattctc tgcattcaga aaaaataaaa
     1981 tctgggattt caacggcggc atccatccac cggagatgaa aacccagtcc aacggtacac
     2041 ccctgcgcca ggtacccctg gcgcagcgtt ttgtcattcc actgaaacag catattggcg
     2101 ctgaaggtga gttgtgcgtt agcgtcggcg ataaagtatt gcgcggccag ccgcttaccc
     2161 gtggtcgcgg caaaatgctg cctgttcacg cgcccacctc gggtaccgtt acggctattg
     2221 cgccccactc tacggctcat ccttcagctt tagctgaatt aagcgtgatt attgatgccg
     2281 atggtgaaga ctgctggatc ccacgagacg gctgggccga ttatcgctct cgcagtcgcg
     2341 aagagttaat cgagcgcatt catcagtttg gtgttgccgg gctgggtggt gcgggattcc
     2401 cgacaggcgt taaattgcag ggcggcggag ataagattga aacgttgatt atcaacgctg
     2461 ctgagtgcga gccgtacatt accgccgatg accgtttgat gcaggattgc gcggctcagg
     2521 tcgtagaggg tattcgcatt cttgcgcata ttctgcagcc acgcgaaatt cttatcggca
     2581 ttgaagataa caaaccgcag gcgatttcca tgctgcgcgc ggtgctggcg gactctcacg
     2641 atatttctct gcgggtgatt ccaaccaaat atccttctgg cggtgctaaa caattaacct
     2701 acattctgac cgggaagcag gttccacatg gcgggcgttc atctgatatc ggcgtattaa
     2761 tgcaaaacgt cggcactgct tatgcagtga aacgtgccgt tattgatggc gagccgatta
     2821 ccgagcgtgt cgtaaccctg actggcgaag caatcgctcg cccgggcaac gtctgggcac
     2881 ggctggggac gccagtgcgt catttattga atgacgccgg attctgcccc tctgccgatc
     2941 aaatggtgat tatgggcggc ccgctaatgg gctttacctt gccatggctg gatgtcccgg
     3001 tcgtaaagat taccaactgt ctgttggctc cctctgccaa tgagcttggc gaaccacagg
     3061 aagaacaaag ctgcatccgg tgtagcgcct gtgctgacgc ctgccctgcg gatcttttgc
     3121 cgcaacagtt gtactggttc agcaaaggtc agcaacacga taaagctacc acgcataaca
     3181 ttgccgactg cattgaatgt ggtgcttgcg cgtgggtttg cccgagcaat attcccctgg
     3241 tgcaatattt ccgtcaggaa aaagctgaaa ttgcggctat tcgtcaggaa gaaaagcgcg
     3301 ccgcagaagc caaagcgcgt ttcgaagcgc gccaggctcg tctggagcgc gaaaaagcgg
     3361 ctcgccttga acgacataag agcgcagccg ttcaacctgc agccaaagat aaagatgcga
     3421 ttgctgccgc tctggcgcgg gtgaaagaga aacaggccca ggctacacag cctattgtga
     3481 ttaaagcggg cgaacgcccg gataacagtg caattattgc agcacgggaa gcccgtaaag
     3541 cgcaagccag agcgaaacag gcagaactgc agcaaactaa cgacgcagca accgttactg
     3601 atccacgtaa aactgccgtc gaagcagcta tctcccgcgc caaagcgcgc aagctggaac
     3661 agcaacaggc aaatgcggaa ccagaacaac aggtcgatcc gcgcaaagct gccgtcgaag
     3721 ccgctattgc ccgtgccaaa gcacgcaagc tggaacagca acaggctaat gcggaaccag
     3781 aagaaccggt cgatccgcgc aaagccgccg tcgaagccgc tattgcccgt gccaaagcgc
     3841 gcaaactgga acagcaacag gctaatgcgg agccagaaga acaggtcgat ccgcgcaaag
     3901 ctgccgtcga agccgctatt gcccgcgcca aagcacgcaa gctggaacag caacaggcta
     3961 atgcggaacc agaagaacag gtcgatccgc gcaaagccgc cgtcgaagcc gctattgccc
     4021 gcgccaaagc acgcaagctg gaacagcaac cggctaatgc ggagccagaa gaacaggttg
     4081 atccgcgcaa agctgccgtc gaagcggcta ttgcacgcgc caaagcacgc aagctggaac
     4141 agcaacaggc taatgcggta ccagaagaac aggttgatcc gcgcaaagcg gcagttgccg
     4201 cggctattgc ccgcgttcag gccaaaaaag ccgcccagca aaaggttgta aacgaggact
     4261 aaatggtatt cagaatagct agctcccctt atacccataa ccagcgccag acatcgcgca
     4321 ttatgctgtt agtgttgctc gcagccgtgc caggaatcgc agcgcaactg tggttttttg
     4381 gttggggtac tctcgttcag atcctgttgg catcggttag tgctctgtta gccgaagctc
     4441 tcgtactcaa actacgcaag cagtcggtag ccgccacgtt gaaagataac tcagcattgc
     4501 tgacaggctt attgctggcg gtaagtattc cccccctcgc gccatggtgg atggttgtgc
     4561 tgggtacggt gtttgcggtt atcatcgcta aacagttgta tggcggtctg gggcaaaacc
     4621 cgtttaatcc ggcaatgatt ggttatgtgg tcttactgat ctccttcccg gtgcagatga
     4681 ccagctggtt accgccacat gaaattgcgg tcaacatccc tggttttatc gacgccatac
     4741 aggttatttt cagcgggcat accgccagtg gtggtgatat gaacacacta cgcttaggta
     4801 ttgatggcat tagtcaggcg acaccgctgg atacatttaa aacctctgtc cgtgccggtc
     4861 attcggttga acagattatg caatatccga tctacagcgg tatgctggcg ggcgctggtt
     4921 ggcaatgggt aaatctcgcc tggctggctg gcggcgtgtg gttgctatgg cagaaagcta
     4981 ttcgctggca tattcccctc agcttcttag taacgctgac gttatgcgca acgttgggct
     5041 ggttgttctc accagaaaca ctggccgcac cgcaaattca tctgctgtct ggagcgacca
     5101 tgctcggcgc attctttatt ttgactgacc cggttaccgc ttctacgacc aatcgtggtc
     5161 gtcttatttt cggcgcgctt gcgggcttat tagtctggtt gatccgcagt ttcggcggct
     5221 atcctgacgg cgtggctttt gccgtcctgc tggcgaacat cacggttcct ctgatcgatt
     5281 actacacccg tccgcgcgtc tacggccatc gcaaagggta aaccatgctg aaaactatcc
     5341 gaaaacacgg cattacgttg gcgctatttg cagcgggttc aacagggtta actgcggcca
     5401 tcaaccagat gaccaaaacg acgattgctg aacaggccag tctgcaacaa aaggcgttat
     5461 ttgatcaggt gctgccagcc gaacgctata acaatgcgct ggcacagagt tgctatctgg
     5521 taactgcgcc agagttaggt aaaggtgagc atcgggttta catcgccaaa caggatgaca
     5581 aaccggtagc cgccgttctg gaagcaaccg cgccagatgg ctattccggt gcgattcagc
     5641 tgctggtggg agccgatttt aacggcacgg tacttggcac gcgcgtgaca gagcaccacg
     5701 aaacgccagg gcttggcgat aaaatcgaac tgcgcctttc tgactggatc acccattttg
     5761 cgggtaaaaa aatcagtggt gcggatgatg cgcactgggc ggtgaagaaa gatggtggtg
     5821 atttcgacca gttcaccggc gcgacgatta ctccccgcgc ggtggttaat gcggtaaaac
     5881 gcgccggatt gtacgctcag acgttaccgg cacaactttc tcaacttcct gcctgtggag
     5941 aataaagcgt gagcgaaatt aaagacgtta ttgttcaggg gttgtggaaa aacaactctg
     6001 cgctggtcca gttgctcggc ctttgtcctc tgttggcggt cacgtccact gccactaacg
     6061 ctctgggttt aggacttgcg actacgctgg tactgacgct gaccaacctg accatttcga
     6121 cgctgcgtca ctggacgcca gccgagatcc gcattcccat ttacgtgatg atcatcgcct
     6181 cggtggtcag cgctgtacag atgctgatca acgcctacgc ttttggcctg tatcaatcat
     6241 tagggatttt tattccgctg attgtcacta actgtatcgt tgtgggccgc gctgaagcct
     6301 tcgccgccaa aaaaggtccg gcgctttcgg cactggacgg cttttcaatt ggtatgggcg
     6361 caacctgcgc catgttcgtg ctgggttcac tacgcgaaat tatcggcaat ggcacgttgt
     6421 ttgacggtgc agattcgctg ttaggtagct gggcaaaagt attacgcgtg gagattttcc
     6481 acaccgactc ccctttcctg ctggcgatgc tgccaccagg tgcatttatt ggcctgggac
     6541 tgatgctggc aggaaaatac ctgattgatg aaagaatgaa aaagcgccgt actgaagcag
     6601 ctgccgaacg tgcattgcca aacggtgaaa cagggaatgt ctgatgaaca aagcaaaacg
     6661 cctggagatc ctcactcgcc tgcgtgagaa caatcctcat cccaccaccg agcttaattt
     6721 cagttcgcct tttgaattgc tgattgccgt actgctttcc gctcaggcaa ccgatgtcag
     6781 tgttaataag gcgacggcga aactctaccc ggtggcgaat acgcctgcag cgatgcttga
     6841 actgggcgtt gaaggggtga aaacctatat caaaacgatt gggctttata acagcaaagc
     6901 agaaaatatc atcaaaacct gccgtatctt actggagcag cataatggcg aggttccgga
     6961 agatcgtgct gcgcttgaag ccctgcccgg cgtaggtcgt aaaacagcca acgtcgtatt
     7021 aaacactgca ttcggctggc cgactattgc tgtcgacacg cacattttcc gcgtttgtaa
     7081 tcgtactcaa tttgcgccgg ggaaaaacgt cgaacaggta gaagaaaagc tactgaaagt
     7141 ggttccggca gagtttaaag tcgactgcca ccattggttg atcctgcacg ggcgttatac
     7201 ctgcattgcc cgcaagcccc gctgtggctc ttgtattatt gaagatcttt gtgaatacaa
     7261 agagaaagtt gacatctgaa gaaaaggggt aacaccgatt accccattga taacctttct
     7321 ttatcctctt ttaaaacatc tttttaacgt caatgatgcc attgcttagc gttatcatc
//
LOCUS       DAZKFU020000229         7378 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZKFU020000229 DAZKFU020000000
VERSION     DAZKFU020000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.231"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..96
                     /locus_tag="ACHOQQ_RS19650"
                     /old_locus_tag="pgaptmp_003930"
     CDS             <1..96
                     /locus_tag="ACHOQQ_RS19650"
                     /old_locus_tag="pgaptmp_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HGU4472128.1"
                     /translation="VLGSLLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            101..634
                     /locus_tag="ACHOQQ_RS19655"
                     /old_locus_tag="pgaptmp_003931"
     CDS             101..634
                     /locus_tag="ACHOQQ_RS19655"
                     /old_locus_tag="pgaptmp_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4472129.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATQVDGKPVVPGMKLSKEKCDQVNAIERDKVLAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            925..1056
                     /locus_tag="ACHOQQ_RS19660"
                     /old_locus_tag="pgaptmp_003932"
     CDS             925..1056
                     /locus_tag="ACHOQQ_RS19660"
                     /old_locus_tag="pgaptmp_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472130.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            1020..1174
                     /locus_tag="ACHOQQ_RS19665"
                     /old_locus_tag="pgaptmp_003933"
                     /pseudo
     CDS             1020..1174
                     /locus_tag="ACHOQQ_RS19665"
                     /old_locus_tag="pgaptmp_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1239..1700
                     /locus_tag="ACHOQQ_RS19670"
                     /old_locus_tag="pgaptmp_003934"
     CDS             1239..1700
                     /locus_tag="ACHOQQ_RS19670"
                     /old_locus_tag="pgaptmp_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4472131.1"
                     /translation="MNRILYVVIIVLLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREAPTTSGVDNATAPRLADTVERDYFTLRERLMLMQKQLEGAQDYIRTQC"
     gene            2000..2806
                     /locus_tag="ACHOQQ_RS19675"
                     /old_locus_tag="pgaptmp_003935"
     CDS             2000..2806
                     /locus_tag="ACHOQQ_RS19675"
                     /old_locus_tag="pgaptmp_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4472132.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            2787..4493
                     /locus_tag="ACHOQQ_RS19680"
                     /old_locus_tag="pgaptmp_003936"
     CDS             2787..4493
                     /locus_tag="ACHOQQ_RS19680"
                     /old_locus_tag="pgaptmp_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4472133.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGACGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            4493..6637
                     /locus_tag="ACHOQQ_RS19685"
                     /old_locus_tag="pgaptmp_003937"
     CDS             4493..6637
                     /locus_tag="ACHOQQ_RS19685"
                     /old_locus_tag="pgaptmp_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4472134.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            6795..>7378
                     /locus_tag="ACHOQQ_RS19690"
                     /old_locus_tag="pgaptmp_003938"
     CDS             6795..>7378
                     /locus_tag="ACHOQQ_RS19690"
                     /old_locus_tag="pgaptmp_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472135.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEYLSPVL"
BASE COUNT         1952 a         1691 c         2058 g         1677 t
ORIGIN      
        1 gtgctgggga gtctgctgtt tgggctgctg acatatctga cgaacctgta tttcaagatt
       61 aaagaagacc ggcgtaaggc ggcgcgggga gagtaagctg atgagcagga aattccgcta
      121 tggtttatca gcggtcgttc tggcgctgat tgctgcaggt gcttctgcgc ctgaaatcct
      181 cgaccagttt cttgacgaaa aagaaggtaa ccacaccacg gcataccgtg atggtgcggg
      241 tatctggacc atctgccgtg gtgccaccca ggtggatggt aagcctgtcg tccccggcat
      301 gaagttgtcg aaggaaaaat gcgaccaggt taacgccatt gaacgtgata aggtgctggc
      361 atgggtggcg aaaaacatca gagtgccact gaccgaacct cagaaagcgg ggatcgcgtc
      421 attctgtcct tacaacattg gccccggtaa gtgtttcccg tcgacgtttt ataaacgaat
      481 taatgcaggc gatcgcaggg gagcgtgtga agcgattcgc tggtggatta aggacggtgg
      541 cagagactgc cgtatccgct caaataattg ctacggtcag gtatcccgtc gcgaccagga
      601 gagtgcgctg gcatgttggg gtatcgacag gtagcagaat attttgctga aaaataacgt
      661 tggtcaacgc ggacggataa cacgaaatcc tgcgaactgg caaaacctaa gtgaataaaa
      721 gtaaaaaccc cgtttttggc agcaagcggg gttttgtgtt tatggcagta agctatgggt
      781 ggctgccttg attgatttta gcaaactgat tagggagttg cgactcatga ttagtcaatt
      841 accaaactgg aagtttttgc tggtctggag catccctttt ttatgggggg tatcccagtt
      901 aattgtggca attaaggggt agctatgtca gacaaactca taacgctggc gaagatcctc
      961 tgtgtaatcg tcggcatttc attttcacta atgctggttg ctatttttct tttcctgccc
     1021 tggatgatgt tgtcttcgtc ggggatgctg gggtaacagt gactgatgac atcagcagag
     1081 cgctggcttt gctattaagt gggtggcggt tggtatcgct gtgtctccga tactgtatgg
     1141 gctggcaaaa ctggtcattg cgctgaaatc gtgaacttta tttaaacaga tgagtaatga
     1201 aattgctgta ctggtccggg caattcgctg gtggtgatat gaaccgtatt ctgtatgtgg
     1261 tgattattgt actgctggtg gcctgtggtg cgcttagtct ggggctgaat cattaccgcg
     1321 ataacgccat cacctacaaa gcgcagcgcg ataaaaaagc cagagagctg gagctagcaa
     1381 acgcaaccat tactgacatg cagcagcgcc agcgtgatgt tgctgcgctt gatgccagat
     1441 actcaaggga attagccgat gcgagagctg aaaatgaaac tctgcgcgct gatgttgccg
     1501 ctggtcgtaa gcgcctgcgg atcaacgcca cctgctccgg taccgtgcgt gaagccccca
     1561 ccacctccgg cgtggataat gcaaccgccc cccgactggc agacacagtt gaacgggatt
     1621 atttcactct cagagagcgg ttgatgctga tgcagaagca gctggaaggg gcacaggact
     1681 atatccgcac tcagtgctga actaagtttt gctgatgcgc cgtatcgtcg ccgtattccc
     1741 gcataaacag agaccgcagc caccttatct gcgtgagtgt gcgggggtaa tcaaaaacga
     1801 tgcacaccgg gttttctcat ttttcacgag atgggagcga tttcccgcga agccgcctgt
     1861 ccggtgcggt ggtggaagaa accggataaa acaacctcat tgtacaaata tcgatcaagt
     1921 atggcgctgc tgtgtgaatt ctgaaaaatc acagcggtca ttatgcgtca gtttttaaca
     1981 gaggacgtca gaaagtgaca tggcaaagct ggactggaaa aagctggagc aggcattccg
     2041 acgcgaacat gccgaaacgg gaataacatt actggactgg tgccggaaga aaaagattaa
     2101 ttacaacacc gccagaaccc gtataaaaat gggcaaaatc gatcatgaaa ttgatcataa
     2161 aaccgatcat gaaatcgatc atgacatctc agatgaagaa cccagcaatg acgcgtgttc
     2221 cgacgatgaa aaatgcgcaa aaaactctga aaaaaactgc gcaaattcgg cagaaacgaa
     2281 acggattcgt ggttcccgac ttttacctcc ttcaaacgct ttttctcagc gaaacaccca
     2341 cgccgtaaga caccgtggat atgcgaagta tcttgaggca gataacctca tggatgatgc
     2401 gtccgacatg gtgctgttcg atgaactggt gttcacccgg gcccgcgcac tttcagtaac
     2461 taaggcactt aaagggatgt tcgccgacct ggaagaggca actgacgtgg aaacccgcgt
     2521 tgctctttac gacaaaatac tcaaagctga acaggccctt gaccggaata ttgcccgtat
     2581 cgagtcaatt gaacgctcat tgctgacgct ggacgtcctg gctgagacag caccaaaact
     2641 tcgtgctgac cgggaaagaa tcaacgccgc cagagataaa ctcagagcgg aaaccgatat
     2701 tctgaccagc cagcgccggg gtgttattac gccagtcagt gacatcgtgt catcgctgca
     2761 tgaaatgagt aattcgggga gactggatga cattccggaa gaataaaacg cgatgtgatg
     2821 aacctgcaga aatgactgag accgaacaac gtctgttcat tatgacaaaa ctgagcaatc
     2881 cctggtggcg cctcaatcat ctctacaaaa tacagaacga aaaaggtgaa ctggtcacct
     2941 tcagaatgcg accggcgcaa cgtcagttgt ttcgtaacat gcataataaa aacattatcc
     3001 tgaaagcgcg ccagctggga ttttccacag ccattgatat ttatcttctc gaccaggcat
     3061 tattcattcc gcatctcaaa tgcgggatcg tcgctcagga taaacaggct gccagtgaaa
     3121 ttttccgcac aaaaattgct gtaccgtttg atcatctccc tgactggctg agagcctcat
     3181 tcaccatcgt tgaacgtcgt agcggtgcct gcggtggcta tatcctgttt ggtcacggct
     3241 cgagtattca ggtggcaacc tcatttcgct caggtacggt gcagcgcctg catatctcag
     3301 agcacggcaa aatttgcgcg aaatatccgg ctaaggcgaa agagctgcga accggtacgc
     3361 ttaatgccgt ctctgatgaa tgcattattt ttgatgaatc cacggctgaa ggcgtgggtg
     3421 gtgattttta cgagatgagt aaccgtgcac aggagagcac tgcatcaggc ttattgctga
     3481 cggcacagga ttataaattc catttttacg catggtggca ggatcctaaa tacagcgcca
     3541 gagtgcctga aagcgggctg aagctgtcac gggaaaaaat gacgtatttt tctgcggttg
     3601 agaaggcaat gaacatcacg cttaccgatg agcagaagca gtggtacatc agtaaggaaa
     3661 ctgaacagcg tgaggaaatg aagcaggagt ttccctcaac gccacaggag gcgtttctga
     3721 cgtccggacg acgtgtgttc agtgccgaaa gtacattgca ggcagaatca ttctgttcgc
     3781 caccgatgat tgtttatgac attgaacctg ttacaggagc gaagactaaa gctcagtctc
     3841 tgcgtgaagg aaataaaaac gagttgcagc ggacgctgat gaattatctg ctggtatggg
     3901 aactgccgga tccggatgaa gagtatgttt gtggggcaga tactgccgaa gggctggagc
     3961 acggagaccg ctcatcgctg gatgttgtca aacgcagtaa tggcgagcag gtggctcact
     4021 ggttcgggca tctcgatgct gaactttttg ctcatctcat ttcgcaggtc tgtcgtatgt
     4081 ataacaacgc gtttgtgggg ccggagcgta ataatcacgg acatgcagtt atcctgaaac
     4141 tccgggaact ctatccgaca cgttatatct acaacgaaca gcatcttgac caggcatatg
     4201 acgacgatac gccccgcctt ggctggctga caacccgtca gagcaaacct gttctgaccg
     4261 aaggaatgaa aacgctcctg aataatggaa tatcagggat ccgctggtca ggcacattat
     4321 cggaaatgaa cacctacgtt tatgacgcga aaggctccat gaatgcacag gaaggctgct
     4381 ttgatgatca gctcatgagc tacatgattg cccaggagat gcgcgccaga atgccggtga
     4441 gggtaaaaca gaaaacggat aaacgcagaa ccacacactg gatggcacac tgatgaaaaa
     4501 tgaaattaac accacagcga tgaaaaacga tcatggatcc acgccgcgtt tttctcagcg
     4561 tcagttactg tctctctgtt ctgatattga cagtcagcct ctctggcgtg atgccgcaaa
     4621 caaggcctgt gcgtattatg atggcgacca gctggcaccg gaagttatcc aggtactgaa
     4681 agatcgcggt cagcccatga ccatccataa cctcattgcc cccacggtag atggtgtact
     4741 gggaatggag gcaaaaacaa gaacggacct gatagtgatg tcagacgatc cgaacgatga
     4801 aacagagaaa ctggcagagg ccattaatgc ggagtttgct gatgcgtgcc gtcttggcaa
     4861 catgaataag gcccgctccg atgcctatgc ggaacaaatc aaggcggggc tcagttgggt
     4921 ggaggtcagg cgcaacagcg atccgttcgg acctgaattc agggtatcca cagtcagcag
     4981 aaatgaggtt ttctgggact ggctcagccg ggaatccgac ctgagtgact gccggtggct
     5041 gatgcgtcgc cgctggatgg ataccgatga ggcaaaagcc acgttcccgg gaatggctca
     5101 ggttatcgat tatgctattg atgactggcg tggttttgtc gataccacga ttactgaagg
     5161 ccagcccagt ccgttgatga gtgcatggga agagtatcag tcatgggatc gcagggagaa
     5221 tgaatggctt cagcgtgaac gccgtcgtgt gctgcttcag gtggtttatt accgtacatt
     5281 cgagcgtctt ccggtgattg aactcagtaa tggacgggtg gtggcttttg ataaaaataa
     5341 tctgatgcag gcggtggctg tggcatccgg gcgggttcag gtgaaagtcg ggcgggtaag
     5401 tcgtattcgt gaagcctggt ttgtgggccc acactttatt gtggatcgcc cctgtagtgc
     5461 tccgcagggg atgtttccgc tggttccttt ctggggatac cgaaaggata aaaccgggga
     5521 gccatacggg ttaatttccc gcgccattcc ggcacaggat gaggtgaatt ttcgtcgtat
     5581 caagctgacc tggctgcttc aggccaaacg cgtgattatg gatgaggatg ccacccagtt
     5641 gtcagacagc gacctgatgg agcagatcga acgtccagat ggcattatta aactgaatcc
     5701 ggcccgaaaa aatcagaaaa gtgtcgcaga tgtttttcgg gttgagcagg attttcaggt
     5761 tgccagccag cagtttcagg tcatgcagga atcggaaaaa cttatccagg ataccatggg
     5821 agtgtattcc gcatttctcg ggcaggattc aggtgcgacg tcaggcgtgg ctatcagtaa
     5881 cctggtggag cagggggcca caacccttgc ggaaatcaac gataactacc agtttgcctg
     5941 ccagcaggtg ggaagactgt tgctggctta tcttctcgat gacctgaaaa aacgccgtaa
     6001 tcatgcagtg gtgattaatc gcgatgatcg ccagcgtcgc cagaccattg tcctcaatgc
     6061 tgaaggtgat aatggtgaac tgaccaatga tatttcaagg ttaaatacac atattgcgct
     6121 ggcgcctgtt cagcagacac cggcgtttaa ggcacagctt gcacagagaa tgtcagaggt
     6181 tattcagggg ctgccgcctc aggtgcaggc tgttgtgctc gacctgtggg ttaatcttct
     6241 ggatgtgccg cagaaacagg agtttgttga gcgtattcgt gctgcgctgg ggacgccaaa
     6301 atcaccggat gaaatgacgc cggaagaaca ggaagtagcg gcacaacaac aggcacttca
     6361 gcaacaacag gcagaactcc agatgcgcga gatggctggc agagtggcaa aactggaagc
     6421 tgacgccgcc agggcacatg cagctgcaca acgggataat gccagtgcac agcgggaagt
     6481 cgccctgaca caggggcagc gttatgtgga tgcgcttaac caggcacata cggcagaaat
     6541 cattaccggc gtacagaata tggaacagga gcaggacgtt cttcagcaac agatgctgta
     6601 tacgttacaa cagcggatga atgaaatgtc gctctgaaaa ctctggcttc aactgaaccc
     6661 cgtcatcgta cggggttttt tgtttccgga ggtaagcgtt ccgggagcgg tgcgcttatt
     6721 cgcgggggca gcgataagcc ttatttactc aaccattcgg atctgtccga taaacagacc
     6781 atgcggagtt atttatggat tttgaattta cgggtgaaga aaccccggaa caactggaaa
     6841 aaatgctgga aggacttggg gatgtggata ttgacagtca cgcacaggac gtcgtgacgg
     6901 aagataccac ggaaaaacat gcggatgagg aagcacagac tcagacgggc gataacaatg
     6961 tggcaccgac gccggatgcc agtgtggagc agacgcagga cgtgaaggag ccggaagcga
     7021 agggggtgct cacccgcgac ggtaaacacg tcattcccta tgaagtcctt gaggctgaac
     7081 gttccggtaa gcaacgggcc gaacaggaag ccgcacttct tcgtgggcag atagctgaag
     7141 aaaaacgcag ggtggaactg ctgacatctc agatccacca ggccggtatg aagcccacac
     7201 cgttaccgga aaacgaaaaa atttctgatg agcagattgc ccgtatcagg gagatgtatc
     7261 cggaaattgg tgacgcggtg gcttcgctca tccgtaaaaa taactatctc cagtcccgtg
     7321 ttcagcaatc aacacagcag gcagaaggta atggtggtga gtatttatca ccggttct
//
LOCUS       DAZKFU020000230         7321 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZKFU020000230 DAZKFU020000000
VERSION     DAZKFU020000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.232"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..378
                     /gene="dinJ"
                     /locus_tag="ACHOQQ_RS19695"
                     /old_locus_tag="pgaptmp_003939"
     CDS             118..378
                     /gene="dinJ"
                     /locus_tag="ACHOQQ_RS19695"
                     /old_locus_tag="pgaptmp_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HGU4472136.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDLFDKLGI"
     gene            381..659
                     /gene="yafQ"
                     /locus_tag="ACHOQQ_RS19700"
                     /old_locus_tag="pgaptmp_003940"
     CDS             381..659
                     /gene="yafQ"
                     /locus_tag="ACHOQQ_RS19700"
                     /old_locus_tag="pgaptmp_003940"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HGU4472137.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLRFERTGTHAALFG"
     gene            815..1555
                     /gene="dpaA"
                     /locus_tag="ACHOQQ_RS19705"
                     /old_locus_tag="pgaptmp_003941"
     CDS             815..1555
                     /gene="dpaA"
                     /locus_tag="ACHOQQ_RS19705"
                     /old_locus_tag="pgaptmp_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGU4472138.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1526..2293)
                     /gene="yafJ"
                     /locus_tag="ACHOQQ_RS19710"
                     /old_locus_tag="pgaptmp_003942"
     CDS             complement(1526..2293)
                     /gene="yafJ"
                     /locus_tag="ACHOQQ_RS19710"
                     /old_locus_tag="pgaptmp_003942"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGU4472139.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETSNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2499..3077)
                     /gene="lpcA"
                     /locus_tag="ACHOQQ_RS19715"
                     /old_locus_tag="pgaptmp_003943"
     CDS             complement(2499..3077)
                     /gene="lpcA"
                     /locus_tag="ACHOQQ_RS19715"
                     /old_locus_tag="pgaptmp_003943"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGU4472140.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3317..5761
                     /gene="fadE"
                     /locus_tag="ACHOQQ_RS19720"
                     /old_locus_tag="pgaptmp_003944"
     CDS             3317..5761
                     /gene="fadE"
                     /locus_tag="ACHOQQ_RS19720"
                     /old_locus_tag="pgaptmp_003944"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGU4472141.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAHSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYKAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKDLGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5804..6250)
                     /gene="ivy"
                     /locus_tag="ACHOQQ_RS19725"
                     /old_locus_tag="pgaptmp_003945"
     CDS             complement(5804..6250)
                     /gene="ivy"
                     /locus_tag="ACHOQQ_RS19725"
                     /old_locus_tag="pgaptmp_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGU4472142.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6431..7201
                     /gene="yafV"
                     /locus_tag="ACHOQQ_RS19730"
                     /old_locus_tag="pgaptmp_003946"
     CDS             6431..7201
                     /gene="yafV"
                     /locus_tag="ACHOQQ_RS19730"
                     /old_locus_tag="pgaptmp_003946"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGU4472143.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLW"
     gene            complement(7253..7321)
                     /locus_tag="ACHOQQ_RS19735"
                     /old_locus_tag="pgaptmp_003947"
                     /pseudo
     CDS             complement(7253..7321)
                     /locus_tag="ACHOQQ_RS19735"
                     /old_locus_tag="pgaptmp_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1741 a         1838 c         1949 g         1793 t
ORIGIN      
        1 tatatttcag tgaattatgt aaatacatca gtttattgaa ttgctcataa attgccgctg
       61 ttgctcaatt gagctacaat tcaagctgaa tatatataca gcacaggaga taccccaatg
      121 gctgcaaatg catttgttcg cgcccgaatc gatgaagatc tgaagaatca ggcggcggac
      181 gtactggccg ggatggggct gaccatctct gacctggttc gcataaccct cacaaaggtc
      241 gcgcgtgaaa aggcattgcc gtttgattta cgcgagccta atcaattaac cattcaatca
      301 atcaaaaaca gcgaagctgg cgttggtgtt cataaggcca aagacgccga tgatttattt
      361 gataaattag gaatttaaat atgattcaaa gggatattga atactcggga caattttcaa
      421 aggatgtaaa acttgcacaa aagcgtcata aggatatgaa taaattgaaa tatcttatga
      481 cgcttcttat caataatact ttaccgcttc cagctgttta taaagaccac ccgctgcaag
      541 gttcatggaa aggttatcgc gatgctcatg tcgaaccgga ctggctcctg atttacaaac
      601 ttaccgataa acttttacga tttgagagaa ctggaactca cgcggcgctc tttgggtaaa
      661 gcccgctgcc gatgaaattt gtaaaccctg cgccgacagg cttgcgtaaa actcttttgg
      721 tgataatgtg agccttccaa atccagacta ttccgatttg ggatccattt tgccgtcctg
      781 gcgtgtgtaa ccgttttatc aaggaataag cagtatgcgt aaaatcgcat taattcttgc
      841 gatgttgttg atcccgtgcg tttcgtttgc cggtttgctg ggcagcagta gctcgaccac
      901 gccagtgagc aaagagtata agcagcagtt gatgggatcc cctgtctaca tccagatctt
      961 caaggaagaa cgtacgctcg atctctacgt caaaatgggt gagcaatatc aactgctcga
     1021 cagctataaa atctgtaaat attccggcgg cttagggcca aaacagcgtc agggcgattt
     1081 caaaagcccg gaagggtttt atagcgtcca gcgtaatcag ttaaaaccag acagccgtta
     1141 ctacaaagcg attaatattg gtttccccaa tgcctatgac cgtgcgcatg gttatgaagg
     1201 gaaatacctg atgatccacg gcgattgtgt ttccatcggc tgctacgcaa tgaccaatca
     1261 gggtattgat gagatcttcc agttcgttac tggtgcgctg gtgtttggtc agccgagcgt
     1321 gcaagtgagt atttacccgt tccgcatgac cgacgctaat atgaagcgcc acaaatattc
     1381 caactttaag gacttctggg agcaactgaa gccaggctac gactactttg agcaaacccg
     1441 taagccaccg accgtttctg tggtcaatgg ccgttacgtg gtcagcaagc cgttaagcca
     1501 cgaagttgtg caaccacaac tggcatcaaa ctacacgctc cccgaggcaa aataagcgcc
     1561 attcgcctgg cataatcttt tgccaggttt cattgcccgt cagcggctgt gtcgcaatca
     1621 ccgtgaccac atcattcggt gtggtctgcg agctgaagtc gatttctaca tcctgatcca
     1681 gcaacgttgc cacgccaaac ggcgcgcggc gggtgatcca gtgtaaatta gtcgagcaat
     1741 acgccattac atagcgaccg tccgaaagca gcatgttgaa aacgcccttc gcccgcagtt
     1801 catccgccag tgaggcgata tatttaaaca ctgccgccat gttgcccggc gtgcgcgggt
     1861 aacgctgcgt taatttatgc aggagccagc aaaaggcttt ttcgctgtcg gtttcaccga
     1921 ccgggcggaa gttgctggtt tccagtgatt tgtagcccgt cagttgcccg ttatgggcat
     1981 aagtccagtt acgcccccat aactcgcggg taaacgggtg agtattttcc agcgccacct
     2041 cgccccgatt agcctggcga atatgagcca ccaccgaaca ggattttatc gggtagtcct
     2101 ggaccagttt ggcgataggt gagttaaagc tgggttgcgg atctttaaat gtgcgacagc
     2161 ctttaccttc gtaaaaggta atgccccagc catctttatg tggcccggtt ccaccaccac
     2221 gctgcaccag accggtgaaa ctaaagcaga tatcggtagg gacgttggcg ctcatcccga
     2281 gcaattcgca catgggtaac ctccacaaca gcgggagcgc atggcccccg gcgaagattg
     2341 atttaagaat tttcaggtcg gatgcggcgt gaacgcatta ttcggcctac ggttcgcgta
     2401 cagatttgta ggcatgataa gacgcgtcag cgtcgcatca ggcatcggtg cacaattgcc
     2461 ggatgcggcg taaacgtctt atccggccta cgccagactt acttaaccat ctctttttca
     2521 atcaactgga tcaggatatg gatcacttta atgtgaatct cctgaatgcg gtcggcataa
     2581 ccaaagtgcg gtacgcgaat ttcgatatcc gccgtgccag ccattttgcc gccgtcttta
     2641 ccggtcaggg tgatcacttt cattcccttc tcacgcgccg ctgcgatcgc tttgatcacg
     2701 tttgcagagt taccggaggt ggagatcccc agcagtacat cgccttcgcg acctaccgct
     2761 tcaacgtaac gggagaaaat atcattgaaa ccgaaatcgt taccgacgca ggaaatatga
     2821 ctaacgtcag aaatagcaat cgccgggtag cccggacggt tttcacggta gcgaccggtc
     2881 aactcttcgg caaagtgcat agcgtcgcaa tgggaaccgc cgttgccgca ggaaagcact
     2941 ttgccaccgg ctttaaagct gtctgctaac aggaccgccg cgcgctgaat ggcgtgaata
     3001 ttggcgtcat cttttaaaaa gttagccagc gtttccgccg cttcgttcag ttcgttacga
     3061 ataagatcct ggtacatgag gatatccttc agcataaatg taatagacaa aatgcagtgt
     3121 accggatacc gccaaaagcg agaagtacgg gcaggtgcta tgaccaggac tttttgacct
     3181 gaagtgcgga taaaaacagc aacaatgtga gctttgttgt aattatattg taaacgcatt
     3241 gctaaatgtt tttacatcca ctacaaccat atcatcacaa gtggtcagac ctcctacaag
     3301 taaggggctt ttcgttatga tgattttgag tattctcgct acggttgtcc tgctcggcgc
     3361 gttgttctat caccgcgtga gcttatttat cagcagtctg attttgctcg cctggacagc
     3421 cgccctcggc gttgctggtc tgtggtcggc gtgggtactg gtgcctctgg ccattatcct
     3481 cgtgccattt aactttgcgc ctatgcgtaa gtcgatgatt tccgcgccgg tatttcgcgg
     3541 tttccgtaag gtgatgccgc cgatgtcgcg cactgagaaa gaagcgattg atgcgggcac
     3601 cacctggtgg gagggcgact tgttccaggg caagccggac tggaaaaagc tgcataacta
     3661 tccgcagccg cgcctgacgg ctgaagagca agcgtttctc gacggcccgg tagaagaagc
     3721 ctgccggatg gcgaatgatt tccagatcac ccatgaactg gcggatctgc cgccagagtt
     3781 gtgggcgtac cttaaagagc atcgcttctt cgcgatgatc atcaaaaaag agtacggcgg
     3841 gctggagttc tcggcttatg cccactctcg tgtgctgcaa aaactctccg gcgtgagcgg
     3901 gatcctggcg attaccgtcg gcgtgccgaa ctcattaggc ccgggcgagc tgctgcaaca
     3961 ttacggcact gacgagcaga aagatcacta tctgccgcgt ctggcgcgtg gtcaggaaat
     4021 cccttgtttt gcgctaacca gcccggaagc gggttccgat gcgggcgcga ttccggacac
     4081 cgggattgtc tgcatgggtg aatggcaggg ccagcaggtg ctgggcatgc gactgacctg
     4141 gaacaaacgc tacattacgc tggcaccgat tgcgaccgtg ctgggtctgg cgtttaaact
     4201 ctccgacccg gaaaaattac tcggcggtgc agaagattta ggcattacct gtgcgctgat
     4261 cccgaccacc acgccgggcg tggaaattgg tcgtcgccac ttcccgctga acgtaccgtt
     4321 ccagaacgga ccgacgcgcg gtaaagatgt cttcgtaccg atcgattaca ttatcggcgg
     4381 accaaaaatg gccgggcaag gctggcggat gctggtggag tgcctctcgg taggccgcgg
     4441 catcacactg ccttccaact caaccggcgg cgtgaaatcg gtagcgctgg caaccggcgc
     4501 gtatgctcac attcgccgtc agttcaaaat ctctatcggt aagatggaag ggattgaaga
     4561 gccgctggcg cgtattgccg ggaacgctta tgtaatggat gctgcggcat cgctgattac
     4621 ctacggcatt atgctcggcg aaaaacctgc cgtgctgtcg gctatcgtta agtatcactg
     4681 tacccaccgc gggcagcagt cgattattga tgcgatggat attaccggcg gtaaaggcat
     4741 tatgctcggg caaagcaact tcctggcgcg tgcttaccag ggcgcaccga ttgccatcac
     4801 cgttgaaggg gctaacattc tgacccgcag catgatgatc ttcggacaag gagcgattcg
     4861 ttgccatccg tacgtgctgg aagagatgga agcggcgaag aacaatgacg tcaacgcgtt
     4921 cgataaactg ttgttcaaac atatcggtca cgtcggtagc aacaaagttc gcagcttctg
     4981 gctgggcctg acgcgcggtt taaccagcag cacgccaacc ggcgatgcca ctaaacgcta
     5041 ctatcagcac ctgaaccgcc tgagcgccaa cctcgccctg ctttctgatg tctcgatggc
     5101 agtgctgggc ggcagcctga aacgtcgtga gcgtatctcg gcacgtctgg gtgatatttt
     5161 aagccagctt tacctcgcct ctgccgttct gaagcgttat gacgacgaag gccgtaatga
     5221 agccgatctg ccgctggtgc actggggcgt acaggatgcg ctgtataagg ctgaacaggc
     5281 gatggacgat ttactgcaaa acttcccgaa ccgcgtggtt gccgggctgc tgaatgtagt
     5341 gatcttcccg accggacgtc attatctggc accgtctgac aagctggatc ataaagtggc
     5401 gaagatttta caagtgccga acgccactcg ctcacgtatt ggtcgcggtc agtacctgac
     5461 gccgagcgag cataatccgg ttggtttact ggaagaggcg ttggtggatg tgattgccgc
     5521 cgacccgatt catcagcgga tctgtaaaga tctgggtaaa aacctgccat ttacccgtct
     5581 ggatgaactg gcgcacaacg cgctggcgaa ggggctgatt gataaagatg aagccgctat
     5641 tctggtgaaa gcggaagaaa gccgtctgcg cagtattaac gttgatgact ttgatccgga
     5701 agagctggcg acgaagccgg taaagttgcc ggagaaagtg cggaaagttg aagccgcgta
     5761 attgaataac ggagccgaaa ggctccgttt ctttatccgc taattattta aaattaaagc
     5821 catccggatg gttttccagg ctgccggtca acgccgcgaa cagcaccgtt ttaccatcaa
     5881 tcgaaagcgc atcgttcaca ttcaaccagg tgagtttctc ttgcgacgtt ttctcatcaa
     5941 tagtcgagaa cagccccgtc atctgattag atttctcgga ccacatcaca gcgatacgtt
     6001 gcgagccaca gtcatgcggt ttgcacgcgc tcatcacctg atacgtctca tctcccaatg
     6061 tcacggtttg tgcgggagta taagtaccgc ctttcatcac ccatgcaggt agcttatgcc
     6121 cttgcaccat ctgattaaac gcagctttgg tggtttcgcc ctttgccagg ctgctaatgg
     6181 ttaaatcatc ctgcgccatt gcactggtgg cgatgaccag agcggcgact gtcgttattg
     6241 ccttaaacat cattcctccc gagcttatcc tgcccatgtt attaacctcc taaagcctgg
     6301 cgataacacg ataatttgca aatcagatat ttccaatatc agcatttgtt atcaatttat
     6361 tctgcgctaa ggcaactgcc tgataaggtg ttaaagttat aaaacccttc gcataaagga
     6421 gttgtacacc gtgcctggtt tgaagattac gcttttgcag caaccgttgg tgtggatgga
     6481 tggtcctgcc aacctgcgtc attttgatcg tcaactggaa ggtattaccg ggcgcgatgt
     6541 gatcgttcta ccggagatgt ttaccagcgg ctttgccatg gaagcggcag cttcgtcgct
     6601 agcgcaagat gacgtagtga actggatgac agctaaagcg cagcagtgca atgcgctgat
     6661 tgcaggcagt gttgcattac aaacggagtc tggttcagtt aaccgctttt tgctggttga
     6721 gccgggcggc acggtacatt tttatgataa gcgtcatctg ttccgaatgg ctgatgagca
     6781 tctgcattat aaagcgggca atgcgcgagt gattgtggaa tggcgcggct ggcgtatttt
     6841 gccgctggtg tgctacgact tacgttttcc tgtgtggtcg cgcaatctca acgattatga
     6901 cctcgccctg tacgtcgcca actggcctgc tccgcgctct ctgcactggc aggcattgct
     6961 gacggcccgc gcgattgaga atcaggcgta tgtggcggga tgcaatcgcg tcggcagcga
     7021 tggcaacggc tgccattatc gcggtgacag ccgggtgatt aatccacaag gcgagattat
     7081 cgctactgcc gacgcgcatc aggcaacgcg cattgatgcg gagctgtcga tgatggcatt
     7141 gcgggaatat agagaaaagt ttccggcatg gcaggatgcg gatgagttta ggttgtggtg
     7201 agtgggggtt gtgtttaggg ccggggagag tcggggtatg actacgaaag cccgctctcc
     7261 tgcgaccact tttatcatag gagtgtggaa gtgcttttcc atcaatcgcg atgacgtctt
     7321 t
//
LOCUS       DAZKFU020000231         7254 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZKFU020000231 DAZKFU020000000
VERSION     DAZKFU020000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.233"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(138..842)
                     /locus_tag="ACHOQQ_RS19740"
                     /old_locus_tag="pgaptmp_003948"
     CDS             complement(138..842)
                     /locus_tag="ACHOQQ_RS19740"
                     /old_locus_tag="pgaptmp_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4472144.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(978..1430)
                     /gene="moaE"
                     /locus_tag="ACHOQQ_RS19745"
                     /old_locus_tag="pgaptmp_003949"
     CDS             complement(978..1430)
                     /gene="moaE"
                     /locus_tag="ACHOQQ_RS19745"
                     /old_locus_tag="pgaptmp_003949"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGU4472145.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1432..1677)
                     /gene="moaD"
                     /locus_tag="ACHOQQ_RS19750"
                     /old_locus_tag="pgaptmp_003950"
     CDS             complement(1432..1677)
                     /gene="moaD"
                     /locus_tag="ACHOQQ_RS19750"
                     /old_locus_tag="pgaptmp_003950"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGU4472146.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1670..2155)
                     /gene="moaC"
                     /locus_tag="ACHOQQ_RS19755"
                     /old_locus_tag="pgaptmp_003951"
     CDS             complement(1670..2155)
                     /gene="moaC"
                     /locus_tag="ACHOQQ_RS19755"
                     /old_locus_tag="pgaptmp_003951"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGU4472147.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2158..2670)
                     /gene="moaB"
                     /locus_tag="ACHOQQ_RS19760"
                     /old_locus_tag="pgaptmp_003952"
     CDS             complement(2158..2670)
                     /gene="moaB"
                     /locus_tag="ACHOQQ_RS19760"
                     /old_locus_tag="pgaptmp_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGU4472148.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2692..3681)
                     /gene="moaA"
                     /locus_tag="ACHOQQ_RS19765"
                     /old_locus_tag="pgaptmp_003953"
     CDS             complement(2692..3681)
                     /gene="moaA"
                     /locus_tag="ACHOQQ_RS19765"
                     /old_locus_tag="pgaptmp_003953"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGU4472149.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3669..3813)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4078..4986
                     /gene="yvcK"
                     /locus_tag="ACHOQQ_RS19770"
                     /old_locus_tag="pgaptmp_003954"
     CDS             4078..4986
                     /gene="yvcK"
                     /locus_tag="ACHOQQ_RS19770"
                     /old_locus_tag="pgaptmp_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGU4472150.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5177..7198)
                     /gene="uvrB"
                     /locus_tag="ACHOQQ_RS19775"
                     /old_locus_tag="pgaptmp_003955"
     CDS             complement(5177..7198)
                     /gene="uvrB"
                     /locus_tag="ACHOQQ_RS19775"
                     /old_locus_tag="pgaptmp_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGU4472151.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1716 a         1949 c         1841 g         1748 t
ORIGIN      
        1 atacttttgt atttatggat acggaaaacg cttccgctcg gccataaggc atattttata
       61 cctggtactt gttaagtgtc gtttttagta agaaaaaaaa tcccggcacc aggccgggat
      121 cagcaggaag aagagaatta acgtcgattg ccaaaaattc gcagcaacat caggaacagg
      181 ttgatgaagt ccagatacaa ggttaacgcg ccaagaatgg aatatttgcg caggttcgac
      241 gcgtcgcggg tatctatctg ctcacccata tttttcagtt tctgggtgtc atacgccgtc
      301 aatccgacaa agacaatcac gccgatgtag gtaactgccc acatcaatgc ttcgcttttc
      361 agccagaagt tgaccagcga tgccagcaca atgccgatta acgccataaa cagcatattg
      421 ccaaagccac ttaaatcgcg cttcgtggtg taaccgtaca ggctcattgc gccgaacatc
      481 ccggcagtaa cgacgaaggt actggcgata gaagcagcgg tataaacaat gaaaatactg
      541 gaaagcgtaa gacccgtcag cgccgaataa agcataaaga gcatcgtcgt tacacctgcg
      601 ctcagctttt gaatcatcgc tgataacaca ataaccaatg ctaattgcgc gatgatcaga
      661 ccgattaaaa agacacggtt agtgaacaac agctccatca cggccgcgga attagccgca
      721 taccaggcaa caaatgcggt cagcaacaag ccaacggtca tccagccata gacttgcgcc
      781 atataagtct gcaagccagc ccggggttgt acaattgaat cagaacgtgg gaatctgtcc
      841 atgacgatct cctgaagata taaggaatat cttaaggata ctgcaaaatg atgaggctgt
      901 gcatcgacgc agcgtaaacg catgtactga gcggtgaaat tgccggacgc agcggtgcct
      961 tatccggcta acaaaaacta ccagcgtttt gccgcctgct gatcgctctc ccgagcttcg
     1021 acccagcggt cgccctccgg cgtggcttcg cgcttccaga acggtgcgcg ggttttgaga
     1081 taatccataa taaactgccc ggcttcaaac gcactgctgc gatgcgcact ggtgacaccg
     1141 acaaaaacga tttcatcgcc cggccataat tccccgatgc ggtgaatcac cgtgacgcgc
     1201 cccagcggcc agcggttacg cgcttcatcg acaatttctg ccagtgcttt ttcagtcatc
     1261 cccggatagt gttcgagggt taatgctttg acgctgtcgc ccaggttatg gttgcgcacc
     1321 ttaccggtaa aggtgactac cgcaccgtct tcgtcacgct ccgccagcca cgggtactct
     1381 tctcctacgc tgaacggctg cggaccaaca acaattttgg tttctgccat cttaacctcc
     1441 ggttaccggc gggaagaaag ctacttcgtc gccgtcagtc agcggatggt caaaactcac
     1501 cagcgtctgg ttgacggcag ccagtaattt gccatcttcc agcgccagcg cccagcgatc
     1561 gctctgcgca gccaggtgct ggcgtaacgc ttcaacggtt gggaaatccg cagccacttc
     1621 ggttgcatct gttcctacca actcgcgcac ctgggcgaaa aaaagaactt taatcatccg
     1681 cttccacctt aaagtcaccc gacttgccgc cgctcttcgc cagcaaacgt accggaccaa
     1741 tcaccatatc tttttgcacc gctttgcaca tgtcataaat ggtcagcgcc gccacggagg
     1801 ccgcggttaa cgcttccatt tcgacaccgg ttttcccggt caggcggcat aaggtttcta
     1861 tacgcacccg attgtgctcc ggctcggcct gtaaattgac ttcaactttg ctgagcatca
     1921 gcggatgaca cagcgggatc agatcccagg tgcgttttgc cgcctgaata ccggcaatac
     1981 gcgcagtggc aaatacgtcg cctttgtggt ggcgaccatc aataatcatc gccagcgtct
     2041 cgctgcgcat ggtgacaaag gcttccgccc gcgcttcacg cacggtttcc gctttggcgg
     2101 agacatccac catgtgcgct tcgccagcgg cgttgatatg ggtcagttgc gacatactta
     2161 tttcttcaaa tgtggatgga aattacacgg acgcgtacgg gcatccagct gcggcgcgat
     2221 gatattttcc catgcggtac ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt
     2281 tttgttagcg acgcccgcta ccgcacgaga ttgcaacgtg gaagtgccaa tctcttcaaa
     2341 cgacaacata cggaacactt caccaaaacc ttcaacttca cggtcgaaca acggcagcaa
     2401 tgcttcggga gcctgatcac cttccgtcag gccagtcccc cccgtaatca acaccacttg
     2461 tacatcgtcg ctggcgatcc acgcagatac ctgagcgcga atagcgtagc ggttttcttt
     2521 cacaatggct ttatcgacaa cgtgatggcc cgcttcttgc gccgaatcgc gcaaatagtg
     2581 accggaggta tcgtcttctt caccgcgacg attagaaacc gtaagaatag caatacgggt
     2641 cgggataaat tcagtgctta cctgactcat ctgatctctc cttttgacgt tttagccgcc
     2701 aatgtacgat aagttttgcg taataccggt gttgttttga tgcaggaaat gggtctgttt
     2761 cttctcccgc agcgccgctg aaatacgcgc ttccagcgcc tgttgctggg tatcgtcttc
     2821 cagcagatcg cgcaggttaa cgccgccttc accaaacagg cagagatgga gtttaccaat
     2881 ggaggaaacg cgcagacggt tgcaagtggc gcagaagtct ttttcatacg gcatgataag
     2941 gccaatctct ccggcgtaat ccggatgaca aaagacttgc gcgggaccgt cgctgcgttg
     3001 acgtaattgg tggatccagc cgcgacgcag tagctcgtca cgcagaacct gaccagagat
     3061 gtgatgctta cggaagagct cgctgccctc gcccgtttcc atcagttcga tgaaacgcag
     3121 ctggataggg cgatgctgga tccagttcag aaaggtgtcg agctggtgat gattaacatc
     3181 acgcatcagc acggtattga ctttgacctt ctcaaaaccg gcctcaaatg cagcatcaat
     3241 ccctgccatc acctggttga atttatcctg cccggtaata gcgtgaaact ggcgggcgtc
     3301 cagactatca acgctgacgt tgatgccagt aagtccagca tcgcgccagt tcgccacatc
     3361 gcgttccaga cggtaaccat tggtggtgac cgctatctgg cgaatagcgt cgttttctcg
     3421 cacagcggcg atgatatcgg taaagtcgcg gcgtaaagac ggctcaccgc ctgtcaggcg
     3481 cactttttcg gtgcccagac tggcgaaggc gcgcgtaacc cggcgaattt catcgacggt
     3541 cagaaagcct ttattggtga cgccgctcgg tttgtagcca tccggcaggc agtaggtgca
     3601 acgaaagtta cacacatcgg taatcgacag gcgcaagtag taaaacttac gcgcaaatgc
     3661 atcagtcagt tgtgaagcca tgtacacctt tccagatacg ggaggcgaag tcatttcttc
     3721 cttcgccctg gtggcaatct ttacgagcat tgtcacggcc aaagcaccat atcagttgac
     3781 ccaggtgcag aggctagagt gtttagtggt taagccgata ctagcgtgta aatgagattt
     3841 ttaccatcca cactttcgct atgtaatcat gtatatagcg tcatgatcgt gctatttcgc
     3901 tacagaataa gggaaaatgg cggttttact ttgatataca tcatcaggga ggcatagcgt
     3961 gcatcgtcct ttagaggtag aagaaaatag tttgtatcct caatattggc aggttaattg
     4021 ctgtttcccc gcaatttgcg ctactgtagc gcggataatc tgactccagg aaactatatg
     4081 cgcaatcgta cgctggctga tcttgatcgt gtcgttgctc tcggcggagg gcatggactg
     4141 ggacgcgttc tctcatcact ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc
     4201 acggataatg gtggctcgac ggggcgtatt cgccgttcag aaggcggcat tgcctggggc
     4261 gatatgcgca actgccttaa ccagctgata actgaaccca gcgtcgcttc cgcaatgttt
     4321 gaatatcgtt tcggcggtaa tggcgaactt tccgggcaca accttggaaa cctgatgttg
     4381 aaggcactgg atcaccttag cgtgcgacct cttgaagcga tcaatctgat tcgaaatctg
     4441 ctgaaagtgg acgcacattt gattccgatg tcggagcatc ctgttgatct gatggcgatt
     4501 gacgatcagg ggcatgaagt ttacggcgag gtcaatatcg accagttaac tacgccgatt
     4561 caagagttat tgttaacgcc taatgtaccc gcaacgcgtg aggcggttca cgctatcaat
     4621 gaagcggatc tcatcattat tgggcctggc agtttttata ccagcctgat gcccattctg
     4681 ctgctgaagg aaatcgcgca ggcattacgc cgcacgccag cgccgatggt ctatatcggc
     4741 aatctggggc gtgagttgag tttacctgcg gccaatttga agctggaaag caagctggca
     4801 attatggagc agtatgttgg taaaaaggtc attgatgcgg tcatcgtcgg gccaaaagtg
     4861 gatgtctcgg cggtgaaaga gcggattgtg attcaggagg tgctggaggc cagcgatatt
     4921 ccgtatcgtc atgatcgcca gttgttacat agcgcactgg aaaaggcgtt acaggcttta
     4981 ggttaacgga aatccgaagg aaaattccgg cttcctattg aagacaaagt gcgcgttgtt
     5041 tatgccggat gcggcgtgaa cgccttatcc ggcctacaaa ccgcgctaat tcaatgtatt
     5101 gcagaatcat gtaggcctga taagcgtagc acatcaggca attttgcttt tgtcatctgt
     5161 cttcttcgtt atcctgtcac gaagccgcga taaacaattc acgcagctga tgcaactggt
     5221 cacgaatttg tgccgcttct tcgaactcca gattctgcgc gtgttgcatc attaaccctt
     5281 ccagctcatg gattttctgt tgcaacgctt taggcgacat atccatcggc acattatccg
     5341 gctcaacaat cgggcgcgat tttcctctgc ccttcgcttt ggttttggcg atgttctgcc
     5401 ccagcgccag gatatcgacc actttcttgt tcaagccttg cggcgtaatg ccgtgttcct
     5461 cgttgtactt ctgctgtttc tcgcggcgac gttcggtttc gccaatcgct ttcgccattg
     5521 atggggtgat cttatcgccg tagagaatcg ctttaccgtt aacgttacgt gccgcacgac
     5581 caatggtctg gatcaacgaa cgttcggaac gcaggaagcc ttctttgtca gcgtcgagga
     5641 tcgccaccag cgaaacctcc ggcatatcca gaccttcgcg cagtaagttg atccccacca
     5701 gcacgtcgaa ctcacccaga cgcaagtcgc ggataatctc catacgttcg acggtgtcga
     5761 tatccgagtg aagataacgc acgcgctcgc catgttcttc gagatattcg gtgagatctt
     5821 ccgccatccg cttggtcagt gtggtgacca gtacgcgttc gttaattgcc gctcgctgac
     5881 gaatctccga aagaagatca tcaacctgtg tcgccactgg ccgcacttcg ataatcggat
     5941 caagcaatcc ggttggacgc accacctgat ccaccacatc gccgccggat ttttccagct
     6001 cataattacc tggcgtcgcc gaaacataga tggtttgcgg cgctaatgct tcgaactctt
     6061 caaatttcag cggacggtta tccagcgctg atggcaggcg gaagccgtac tccaccagtg
     6121 tctctttacg cgcccggtca ccgcgataca tgccgccaat ttgtggaatg gtgacgtgag
     6181 attcatcgac caccagcagc ccatcggcag gcaggtaatc aaacagcgtc ggcggtggct
     6241 cacccggtcc acgaccagag aggaagcgcg agtagttttc aatccccgaa cagtagccca
     6301 gctcgttcat catctccaga tcaaactggg tacgctgggt cagccgctgc tcttccagca
     6361 gtttgttgtt ttccaacagc actttgcgtc tggcggccag ctcttctttg atctcctcca
     6421 tcgcctgtac gatgcgctcg cgcggtgtga cgtagtgcgt tttcgggtag atggtaaaac
     6481 gtggaatagt ggaaacaatc tgcccggtca gcgggtcaaa taacgacaat cgttccactt
     6541 cctcgtcaaa cagttccacg cgaagtgcaa tgtcatccga ttctgccggg aatatatcaa
     6601 tcacctcgcc acgaacgcgg aaagtaccgc gctggaatgc ctgatcatta cgagcgtatt
     6661 gcagctccgc cagtcggcgc agaatcgcgc gctgatcgat aatcataccg accgtgagat
     6721 ggagcatcat cttgagatat aaatcaggat cgcccagacc ataaatcgcg gaaacagacg
     6781 ccaccacaac cacatcacgt cgctccagca tcgctttggt ggcggacaaa cgcatctgtt
     6841 cgatatgttc gtttaccgag gcatctttct caatgaaggt gtcagaactc ggtacatagg
     6901 cttccggctg atagtagtcg tagtaggaga cgaaatattc caccgcgttt tccgggaaga
     6961 actctttcat ttcgccatac agctgggccg ccagcgtttt gttgggcgca agtaccatgg
     7021 tagggcgctg aaggtcagca atgacattgg caatggtgaa ggttttcccg gagccagtca
     7081 cgccaagtaa cgtctggtgc gccaggccat cttccagccc ctcttcgaga cgtcgaatcg
     7141 cctctggctg atcgccagaa ggtttaaaag cggaattcag tttgaacggt ttactcatga
     7201 gtctctacct gaaggagttg ggcgggcagg tatgtaattt tactcgtcgt actt
//
LOCUS       DAZKFU020000232         7185 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZKFU020000232 DAZKFU020000000
VERSION     DAZKFU020000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.234"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            319..1233
                     /gene="shuT"
                     /locus_tag="ACHOQQ_RS19780"
                     /old_locus_tag="pgaptmp_003956"
     CDS             319..1233
                     /gene="shuT"
                     /locus_tag="ACHOQQ_RS19780"
                     /old_locus_tag="pgaptmp_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HGU4472152.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVAPHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRITDVVESLHQQLWPQ"
     gene            1253..2590
                     /gene="hutW"
                     /locus_tag="ACHOQQ_RS19785"
                     /old_locus_tag="pgaptmp_003957"
     CDS             1253..2590
                     /gene="hutW"
                     /locus_tag="ACHOQQ_RS19785"
                     /old_locus_tag="pgaptmp_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HGU4472153.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDEAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            2603..3097
                     /gene="hutX"
                     /locus_tag="ACHOQQ_RS19790"
                     /old_locus_tag="pgaptmp_003958"
     CDS             2603..3097
                     /gene="hutX"
                     /locus_tag="ACHOQQ_RS19790"
                     /old_locus_tag="pgaptmp_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HGU4472154.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPFSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            3097..3720
                     /gene="chuY"
                     /locus_tag="ACHOQQ_RS19795"
                     /old_locus_tag="pgaptmp_003959"
     CDS             3097..3720
                     /gene="chuY"
                     /locus_tag="ACHOQQ_RS19795"
                     /old_locus_tag="pgaptmp_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HGU4472155.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRVQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            3769..4761
                     /gene="shuU"
                     /locus_tag="ACHOQQ_RS19800"
                     /old_locus_tag="pgaptmp_003960"
                     /gene_synonym="chuU"
     CDS             3769..4761
                     /gene="shuU"
                     /locus_tag="ACHOQQ_RS19800"
                     /old_locus_tag="pgaptmp_003960"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HGU4472156.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4758..5528
                     /locus_tag="ACHOQQ_RS19805"
                     /old_locus_tag="pgaptmp_003961"
     CDS             4758..5528
                     /locus_tag="ACHOQQ_RS19805"
                     /old_locus_tag="pgaptmp_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HGU4472157.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5580..6227)
                     /locus_tag="ACHOQQ_RS19810"
                     /old_locus_tag="pgaptmp_003962"
     CDS             complement(5580..6227)
                     /locus_tag="ACHOQQ_RS19810"
                     /old_locus_tag="pgaptmp_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGU4472158.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQIRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(6291..6617)
                     /gene="hdeB"
                     /locus_tag="ACHOQQ_RS19815"
                     /old_locus_tag="pgaptmp_003963"
     CDS             complement(6291..6617)
                     /gene="hdeB"
                     /locus_tag="ACHOQQ_RS19815"
                     /old_locus_tag="pgaptmp_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGU4472159.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(6733..7065)
                     /gene="hdeA"
                     /locus_tag="ACHOQQ_RS19820"
                     /old_locus_tag="pgaptmp_003964"
     CDS             complement(6733..7065)
                     /gene="hdeA"
                     /locus_tag="ACHOQQ_RS19820"
                     /old_locus_tag="pgaptmp_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGU4472160.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT         1813 a         1796 c         1730 g         1846 t
ORIGIN      
        1 ggttatgaaa cctatcctgc gccctgtaaa ccaccttgtt tttatggtta tccccccatg
       61 cctcatcact aaattttgtc gttaacagtg ataatttttt cttctctttg ctctgcaagc
      121 ctcgatatct ctgggttctc agctttttcc cgcgatgaaa tttagtcggt aacaagaacg
      181 ttagctgacg aagcatcttg ataaaaatca aaaataaata cgcttgtaac gccttgcgta
      241 ttgaatcgac ggttgtattt catatataac tcaaatgata atcatgatca ttctcaattt
      301 gataagagtt ctcatattat gccaaggatc atcaccaggc cgtttctttt ttctccactg
      361 acgttatgta tttcagccgt ggcctctgcc gcgaagaccg cagtaaaacg aaaaaaattg
      421 tttacggcag tactggctct tagctgggct tttagcgtaa cggctgccga acgtatcgtg
      481 gtcgcaggag gatcgctgac ggagctaatc tacgcgatgg gcgctggcga acgcgtggtt
      541 ggtgtcgatg aaacgacatc ttatccacca gaaaccgcca aactgccgca tattggctac
      601 tggaaacaac tcagcagcga aggcattttg tcacttcgcc cggatagcgt aattacctgg
      661 caggatgccg ggccacaaat tgtgctcgac caactacgag cgcagaaagt caacgtcgtc
      721 accctgccgc gtgtgccagc cacccttgag cagatgtacg ccaacattcg ccaactggca
      781 aaaacgctac aggttcctga acaaggcgag gcgctggtga cacaaatcag ccaacgcctg
      841 gagcgagtac agcaaaacgt ggcggccaaa aaagccccgg ttaaagccat gtttattctc
      901 tccgcaggcg gaagtgcgcc acaagttgcc ggtaaaggca gcgtcgcgga cgccattctt
      961 tcgcttgccg gagcagaaaa cgtcgctccc caccagcagt acaaaagcta cagtgcagaa
     1021 tcactgattg cggctaaccc tgaagtgatt gtcgtaacct cacaaatggt cgatggcgat
     1081 attaatcggc tacgttctat tgctggaatt acccacactg ccgcatggaa aaaccagcgc
     1141 attatcaccg ttgatcaaaa cctgattctg ggaatgggtc cgcgtattac tgatgtcgtt
     1201 gagtctttac accagcagct ttggccgcaa taaatggatt taaatcagac gaatgaacgc
     1261 aaataacacc ctggatctca cgccacattt tgctttagac ggtgatcagc catttaaaga
     1321 caggcgcgcc atgatgccgt ttcgtggggc cattccagtt gccaaagagc aactggcaca
     1381 gacctggcaa gagatgatca atcaaacggc atcacctcgt aagcgactgg tttacctaca
     1441 cattcctttt tgtgcgacac actgcacgtt ttgtggtttt tatcagaacc gttttaatga
     1501 agatgcgtgt gctcattata ccgacgccct gattcgtgaa atagaaatgg aagcggacag
     1561 cgtattgcat cagtctgccc ccatccacgc ggtctatttc ggtggcggta cgccttccgc
     1621 actttcggca cacgatttgg ccagaatcat caccacatta cgagaaaagc tgccactggc
     1681 ccctgattgt gaaatcacta tcgaaggacg ggtattgaat tttgacgctg agcgaatcga
     1741 tgcttgcctt gatgcaggag caaaccgctt ctcaattggt atccagtcgt ttaacagcaa
     1801 aatccgcaag aaaatggccc gcacctcgga tggcccaacc gctattgcgt ttatggaaag
     1861 cctggtcaaa cgtgaccgtg ctgcggtggt ctgtgacctg ctttttggcc tgccgggtca
     1921 ggatgcgcaa acctggggag aagatctggc tattgcccgc gatatcggtc tcgatggcgt
     1981 cgatctctat gcgctcaatg tcctgcccaa tacaccgctg ggcaaagccg tggaaaatgg
     2041 gcgtactacc gtgccctctc cggcagaacg tcgcgatctt tatctgcaag ggtgtgactt
     2101 catggacgaa gccggttggc gctgtattag taacagccac tggggccgta ccacacgcga
     2161 acgcaatctc tataacctgc tgataaaaca aggtgccgat tgtctggcct ttggttccgg
     2221 agccggcggt tcgattaatg gttactcctg gatgaacgaa cgcaatcttc agacctggca
     2281 tgaatccgtc gcggcaggca aaaaaccgct gatgatgatc atgcgtaacg ccgaacgtaa
     2341 tgcgcaatgg cgtcatacct tgcagtcagg tgttgaaaca gcgcgtgtac cgctggatga
     2401 acttacgcca catgccgaaa aactcgcgcc gttactggct caatggcacc aaaaaggctt
     2461 aagtcgcgat gcttcaactt gcctgcggct gactaatgaa ggtcgtttct gggcaagcaa
     2521 tattttgcag tctcttaatg aactaattca ggtacttaat gcgccagcga ttgtgcgtga
     2581 aaaaccataa caggagtgat ttatgagcca tgtctcgtta caagaatttt tgaaaacgga
     2641 gccggacggt acgctggagg tggtagccga acaatacaac accacactgc tggaggtagt
     2701 gagaaatcta cccttctcta ccgtagtgcc aggcgataaa ttcgatactg tctgggatac
     2761 cgtctgcgaa tggggcaacg tcaccacgtt agtacatact gccgatgtaa tcctcgaatt
     2821 tagcggcgaa ctgccttcgg gtttccatcg ccacggctat ttcaacctac gcggaaagca
     2881 cggcatgtcc gggcatatca aagcagaaaa ctgcacgcat attgccttaa tcgaacgtaa
     2941 atttatgggc atggacaccg cgtccattct ctttttcaac aaagaaggca gcgccatgct
     3001 gaaaatcttc ctcggcaggg atgaccatcg tcagctcctg agcgaacaag tcagcgcctt
     3061 ccatactctg gcggcatcgt taaaggaaca cgcctgatga cgccgtggct actctttggc
     3121 gcaggcggaa aaggtgtggg tgccagaacg cttgaactgg cgctggcgga acaacgtccg
     3181 gtcgttgctg tcattcgtca tgccgatgcc gccacgaaac tggcgcaaca aggcgtacag
     3241 gtttttacag gcgacgcctg tgatgccagc gtagtcgcgg ctgcttgccg cgcagctggc
     3301 ccggatgcgc ttatcatctc aacaatggga ggagctcagg attatctggc gcacagaaca
     3361 gtgattgatg aggccgaaaa ggccggcatc agtcgcatga ttctggtgac ctctttggga
     3421 tgcggcgata gctggccgtt tttatcggag cgggcaaagg ctgcgtttgg tcaggcggta
     3481 cgggaaaaaa cgctggcgga aagctggctg caaaccagcc agctagacta cgccattctc
     3541 cgccctggtg gtctgcttga cggcgcagca acaggcaaag cacaacgggt acaaaatcag
     3601 gagtgtcatg gttttgtccg ccgtgccgat gtcgccgcac atatccatga actggcaaat
     3661 gcgccagcac ttaaccagca ggtctacagt ctgattgaac cggacctgaa accggcgtga
     3721 taccccggcg cggcatgtcc gcgccgtaat gtcgtaacgg agttttgcgt gctcaaggat
     3781 tcattttcat ccgccagggt ctttatgggg ctatcactat tattgcttgc cctggtgctg
     3841 ttcggtgcca gtcagggagc gttaaagatc agttttgatg ccctttttga tgaggaatac
     3901 cgcgatatct ggctcaatat tcgtctacca cgggttttgc tggcggtgct ggtaggtgca
     3961 gcgttagcaa ccgcaggggt aattatgcag gggctttttc gcaacccaat ggctgaccca
     4021 ggattacttg gcgtcagtag cggttctgca ttaatggttg gcgttgctat tgtgctgccc
     4081 ttctccttcc cagtggttct ggtgctctat gagcaaatgg tgtttgccat tgccggaagt
     4141 ttagtagtct gcaccatcat ttttctcatc acgcagcgcc atcgcgatgg cagcatgatg
     4201 caattattac tcgccggtat cgccatcaat gccctgtgcg gcgcagcgat cggcatcctg
     4261 agctatatcg gcgatgagca gcagctacga caactcacat tgtggatgat gggcaatctt
     4321 ggacaggcgc aatggccgac gttattggtt gccagttcat tcatccttcc ggccattatc
     4381 gcaacaactt gtctcgccgg aacgctgaat ttactgcagc tcggtgatga agaagcccat
     4441 tacctcggcg tgaacgttaa gcgtaaacgc cagcaattac tgttagtgag ctcactgctc
     4501 gttggtgctg ccgtatcggt aagcggcatt atcggcttta ttggcctggt gatcccgcat
     4561 ctgattcgca tgactaccgg agcaaatcac cgctggctaa tcccttgttc cgccctcgcc
     4621 ggagcctgtt tattgctgat ggcagacacg cttgcccgca cgctggtaca gccagcagaa
     4681 atgcccgtgg gattattaac cagcctgctt ggtggccctt attttatgtg gttgattctg
     4741 cgaaatcgga ggatcacatg atcagcgccc agaatctggt ttatagcctt caggggcggc
     4801 gtttgactga taatgtctcg ctgacttttc ccggtgggga aattgttgcc attctcggcc
     4861 ctaatggtgc gggaaaatct acgctgcttc gccagttaac cggctatctt caacctgata
     4921 gcggcgaatg tcggttgttt aataagccat taaatgaatg gtcgattact gaactggcga
     4981 aacaccgggc cgtgatgcgt caaaatagcc acatggcgtt tccattcagc gtacaagaag
     5041 ttatccagat ggggcggcat ccgcatcgta ctggtaatca ggataatgaa acagcacaaa
     5101 taatggcgtt atgtgactgt caggcgttag caaaccgcga ctatcggcaa ttatccggtg
     5161 gtgaacaaca gcgcgtgcaa ctagcgcgcc tgctggtgca actctgggaa cctactccct
     5221 caccgaaatg gttatttctt gatgaaccaa cttcggcgct ggatattcac catcagcagc
     5281 acttgtttcg tttattacgc cagttagttc acgagcggca gttcaatgtc tgctgcatat
     5341 tgcacgatct aaatcttgcc gcgcgttatg cagaccgtgt tgtattaatg caaaaaggaa
     5401 aggttatcgc aaatggtaaa ccacaggacg tattgacaca gcaggcgctg acaatgctct
     5461 acggcgcgga tattaccgta cttaaagatc ctgccaacca ttcaccactg atcgtcctcg
     5521 atcattaaaa gcaacaggag taccgcactg gtactcctgc gaataaataa ctatacagat
     5581 tagctaattg aaacgccttt cacccctgcc atacctttta ataatcgcaa cagatcttct
     5641 atcgacgttg tggcatgaag ctggatatca atggcgacca cttcatgatc ttcattttcc
     5701 tgcaacgaaa ctaaatcagt ttttattttt tgctgcttaa accagtcgag cattgatacc
     5761 gtattaccgt tcactagtgt cagctgtaaa tggtaatttt tgttcatcag gcggaaggtt
     5821 aattgatgga agacttccag caccagcaag gtcatcactg aaccataaat acccagttcg
     5881 tacataccac tgccaataac cataccaatg gcggcggtca cccagatatc cgctgcagtc
     5941 gtcagaccta caatattttg gttacgaacg agaatgttac ctgcaccgat aaacccgacg
     6001 cccgtcacca cctgagcagc aatacggctg gggtccagtc cgacgtgatc taaagacagc
     6061 acgtcagcaa aaccatattt cgaaacaatc ataaacaggg cgcttcccat gccaattaat
     6121 acatgtgtgc gtaaccctgc tcctttgccg cgtatttgcc tttccatgcc aatagcgcca
     6181 caggcaattg ccgcgagtat aagacgtata ataaattctg ctgtcatata aaaatgcaaa
     6241 aaggagcagc aagatggctc aacttgctac tcctttttac ttgtacctca ttaattcggc
     6301 aagtcattag atgcttgatt tttgaaggta tacaaatttt tctgcgggtt tttcttacag
     6361 tattcgatca ctttaggaat ttgagtgaga tcggtttcat ttaaagtaac ggtatcgcca
     6421 cctttatata ctgtttcttc atgcagcatc caccatgcaa ccggggtcat tgcttttgga
     6481 ttcagatcaa taaattcctg gcaggtcata tctttagcgg attcattggc tgccagcgca
     6541 gattgtgcgt tcaccagtga caaagctgct acagcgccca taaaaataaa cgctttacgg
     6601 agagatgaaa tattcatttt gtaacccatt caatatagag attatatatg aacaggagga
     6661 atagctgctt ttaaagaaga tattccggcc tgttcaatat gcaaggaagt acgatgtaac
     6721 gcattcccgg aattacatat ctttcttaat tttgtcccat tcgcctttaa ctttatcttt
     6781 aaagttggct tgtttatcct gagtacaagc ctgaacgata gctggggtta cggttgcaat
     6841 accctgaaca tctaaaaccg catcttctgg tttatctttg ttgttcagcg cttcagcaaa
     6901 accaactgca gttggctgga aggattcgtc cacagccagg aaatcttcac aggtccagga
     6961 gttgaccggt tttttgttat cagctgcttt ttgcgcatcc gctgcattgc tcacaactgg
     7021 cagaagaagc agaccaccaa gaataacgcc taatactttt ttcatcgtaa tatcctcaac
     7081 tataaagtga aagagccgtc acgaatcaat ttcgacactg aggttataac ctggttttct
     7141 gtatatgtca tgttgatgga aaatatcaaa atcagatatt tttat
//
LOCUS       DAZKFU020000233         7180 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZKFU020000233 DAZKFU020000000
VERSION     DAZKFU020000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.235"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..122)
                     /gene="yneK"
                     /locus_tag="ACHOQQ_RS19825"
                     /old_locus_tag="pgaptmp_003965"
     CDS             complement(<1..122)
                     /gene="yneK"
                     /locus_tag="ACHOQQ_RS19825"
                     /old_locus_tag="pgaptmp_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGU4472161.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTI"
     gene            complement(200..1081)
                     /gene="yneJ"
                     /locus_tag="ACHOQQ_RS19830"
                     /old_locus_tag="pgaptmp_003966"
     CDS             complement(200..1081)
                     /gene="yneJ"
                     /locus_tag="ACHOQQ_RS19830"
                     /old_locus_tag="pgaptmp_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4472162.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            1182..2570
                     /gene="sad"
                     /locus_tag="ACHOQQ_RS19835"
                     /old_locus_tag="pgaptmp_003967"
     CDS             1182..2570
                     /gene="sad"
                     /locus_tag="ACHOQQ_RS19835"
                     /old_locus_tag="pgaptmp_003967"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGU4472163.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIAAAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2634..3560
                     /gene="glsB"
                     /locus_tag="ACHOQQ_RS19840"
                     /old_locus_tag="pgaptmp_003968"
     CDS             2634..3560
                     /gene="glsB"
                     /locus_tag="ACHOQQ_RS19840"
                     /old_locus_tag="pgaptmp_003968"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGU4472164.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3560..3919
                     /gene="yneG"
                     /locus_tag="ACHOQQ_RS19845"
                     /old_locus_tag="pgaptmp_003969"
     CDS             3560..3919
                     /gene="yneG"
                     /locus_tag="ACHOQQ_RS19845"
                     /old_locus_tag="pgaptmp_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGU4472165.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            4058..5476
                     /locus_tag="ACHOQQ_RS19850"
                     /old_locus_tag="pgaptmp_003970"
     CDS             4058..5476
                     /locus_tag="ACHOQQ_RS19850"
                     /old_locus_tag="pgaptmp_003970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4472166.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            5703..7154
                     /gene="uxaB"
                     /locus_tag="ACHOQQ_RS19855"
                     /old_locus_tag="pgaptmp_003971"
     CDS             5703..7154
                     /gene="uxaB"
                     /locus_tag="ACHOQQ_RS19855"
                     /old_locus_tag="pgaptmp_003971"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGU4472167.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1718 a         1681 c         1931 g         1850 t
ORIGIN      
        1 attattgtac taagtgcatc aataaaatcc tttaaaactt cttttacctg cggtgcaata
       61 ttacgaacgg gaaaatcatc aggaagagat atgtaattgc tgatgcttac tggcgtaacc
      121 atatttgccc catatagtca taagtcatcg gaacatcact atatagagca tattagccgc
      181 aagaactatc aaaaatagct cattggtaac cctgttttgt cgagtcaggc acatccagta
      241 gttgaataaa tgcctcgagc ggacgcgttt tcgcaccacg acgccagacc agccaggttg
      301 ttagccaacg ccattgctca gctaacggcc acgcttcaac ctggtgatgc cccggcatac
      361 tttccagcat agagcgcgga ataagcgcaa tacctgctcc tgcgaccaca caggccaaca
      421 ttccgtgata agactccatc tcatggatag ttcccggagc ggcaccgtca gcatgaaacc
      481 agctctcgaa gtggcgacga tacgaacaat tggcgcggaa ggcataaatg ttactgccat
      541 taacctgact ggcacggatt actggcgcat gaccttgtgg cgtgacgatc atcagctcct
      601 cgcggtatac cggtatcccg tcaatggcag tatggttaat gggtccatca ataaacgccg
      661 cattaagttt tccctccagc acaccttcca gcatagtgcc ggaagggccg gtggaaagtg
      721 aaaactgaat tttgggataa cgacggttga attccgccag cgtggccgga atgcgaactg
      781 ctgcggtgct ttccagcgaa cccagagaaa ataaaccttg cggctcatcg ccagcgacaa
      841 cgctccgcgc ttcatccact aacgcgagaa tttgttggct atagcgtaaa aagttatgcc
      901 cggcaggaga tagacgtaaa cgctgattct cgcgaataaa cagatctacc cccagttctg
      961 tttccagctg gcgtagacgg gtagtgagat tcgacggcac gcgatgcact attgctgcag
     1021 cctgcgtaat gctgccagct tcggcaaccg cgttgaacat ctccagttgg gtcagatcca
     1081 taatattctc gattcgtgaa taagtggctt aatattattc attttaaagc aagagtaaat
     1141 ctgcgtatct tcataccatg actcataaag gagatacccc gatgaccatt actccggcaa
     1201 ctcatgcaat ttcgataaat cctgccacgg gtgaacaact ttctgtgttg ccgtgggctg
     1261 gcgctgatga tatcgaaaac gcacttcagc tggcggcagc aggctttcgt gactggcgcg
     1321 agacaaatat agattatcgt gctgaaaaac tgcgtgatat cggtaaggct ctgcgcgccc
     1381 gtagcgaaga aatggcgcaa atgatcaccc gcgaaatggg caaaccaatc aaccaggcgc
     1441 gcgctgaagt ggcgaaatcg gcgaatttgt gtgactggta tgcagaacat ggtccggcaa
     1501 tgctgaaggc ggaacctacg ctggtggaaa atcagcaggc agttattgag tatcgaccgt
     1561 tggggacgat tctggcgatt atgccgtgga attttccgtt atggcaggtg atgcgtggcg
     1621 ctgttcccat cattcttgct ggtaacggtt acttacttaa acatgcgccg aatgtgatgg
     1681 gctgtgctca gctcattgcc caggtgttta aagatgcggg aatcccacaa ggcgtatatg
     1741 gctggctgaa tgccgacaat gacggtgtca ctcagatgat taaagactcg cgcattgctg
     1801 ctatcacggt gaccggaagt gttcgtgctg gagcggctat tggtgcacag gctggagcgg
     1861 cactgaaaaa atgcgtactg gaactgggcg gttcggatcc atttattgtg cttaacgatg
     1921 ccgatctgga actggcggtt aaagcggcgg tagccggacg ttatcagaat accggacagg
     1981 tttgtgcagc ggcaaaacgc tttattatcg aagagggaat tgctgcggca tttaccgaac
     2041 gttttgtggc agctgcggca gccttgaaaa tgggcgatcc ccgtgacgaa gagaacgctc
     2101 tcggaccaat ggctcgtttc gatttacgtg atgagctgca tcatcaggtg gagaaaaccc
     2161 tggcgcaggg tgcgcgtttg ttactgggcg gggaaaagat ggctggggca ggtaactact
     2221 atccgccaac ggttctggcg aatgttaccc cagaaatgac cgcgtttcgg gaagaaatgt
     2281 ttggccccgt tgcggcaatc accattgcga aagatgcaga acatgcactg gaactggcta
     2341 atgatagtga gttcggcctt tcagcgacca tttttaccac tgacgaaaca caggccagac
     2401 agatggcggc acgtctggaa tgcggtgggg tgtttatcaa tggttattgt gccagcgatg
     2461 cgcgagtggc ctttggtggc gtgaaaaaga gtggctttgg tcgtgagctt tcccatttcg
     2521 gcttacacga attctgtaat atccagacgg tgtggaaaga ccggatctga cgcgacatat
     2581 tcagctctga tatactcgca ggtcttttca gacctgcggt ccaggagtag aaagtggcag
     2641 tcgccatgga taatgcaatt ttagaaaaca tcttgcggca agtgcggccg ctcattggtc
     2701 agggtaaagt cgcggattat attccggcgc tggctacagt agacggttcc cgattgggga
     2761 ttgctatctg taccgttgac ggacagcttt ttcaggccgg agacgcgcaa gaacgttttt
     2821 ccattcagtc tatttccaaa gtgctgagtc tcgttgtcgc catgcgtcat tactccgaag
     2881 aggaaatctg gcaacgcgtc ggcaaagatc cgtctggatc accgttcaat tctttagtgc
     2941 aactggaaat ggagcagggt attccgcgta atccgtttat taatgccggt gcgctggtgg
     3001 tctgcgatat gttgcaaggg cgattaagcg caccacggca acgtatgctg gaagtcgtgc
     3061 gcggcttaag cggtgtgtct gatatttcct acgatacggt ggtagcgcgt tccgaatttg
     3121 aacattccgc gcgaaatgcg gctatcgcct ggctgatgaa gtcgtttggc aatttccatc
     3181 atgacgtgac aaccgttctg caaaactact ttcattactg cgctctgaaa atgagctgtg
     3241 tagagctggc ccggacgttt gtctttctgg ctaatcaggg gaaagctatt catattgatg
     3301 aacctgtggt gacgccaatg caggcacggc aaattaacgc gctgatggcg accagtggta
     3361 tgtaccagaa cgcgggggag tttgcctggc gggtgggact accggcgaaa tctggcgttg
     3421 gtggcggtat tgtggcgatt gttccgcatg aaatggccat cgcagtctgg agtccggaac
     3481 tggatgatgc aggtaactcg cttgcgggta tcgccgtcct tgaacaattg acgaaacagt
     3541 tagggcgttc ggtttattaa tgcagtctct cgatccactc ttcgcgcgtt tatcccgttc
     3601 aaaatttcga tctcgctttc gtttgggcat gaaagagcgt cagtattgtc tggagaaagg
     3661 cgcgccagtc atcgaacaac atgcggcgga ttttgtcgct aaacgtcttg ctccggcatt
     3721 accggctaat gacggtaagc aaacccccat gcgcgggcat ccggtgttta tcgctcagca
     3781 cgccaccgcg acctgctgtc gcggttgtct ggctaagtgg cataacattc cgcagggtgt
     3841 ttcgctaagc gaagaacaac aacgttatat cgtggcggta atctatcact ggctggttgt
     3901 acagatgaat cagccatgag aattgcggaa aacgacaaaa tagcattccc agggtacctt
     3961 tatttgccgc cgctgtcagc gaacatgcta tcctttatga gaatttactt attacgttga
     4021 gataattgcc acccgcaatt gtatggacag ataattaatg catgtacacc ccatttcaac
     4081 tttccgtgtg ttccaggaag gtcatctgct acgtaatagc atcgctattt ttgcgctaac
     4141 cacgctgttc tattttattg gtgcagagtt acggctggtt cacgaacttt ctcttttctg
     4201 gccgctgaat ggcgtaatgg cgggggtgtt tgcccgctat gtctggctta atcgactgca
     4261 ttactatgcg atcagttatg tggcgatgct ggtttatgat gccataacca ccgaatgggg
     4321 actggtttca ctggctatca atttctccaa tatgatgttt attgttaccg tcgccttact
     4381 ggtcgtgcgg gataagcgtt tggggaaaaa taaatatgag ccagtaagtg ccttacggct
     4441 atttaattac tgtctgattg ccgcattatt atgcgctatt gtcggggcga ttggttcggt
     4501 cagtattgat agtctggatt tctggccttt gcttgcggac tggttcagtg agcaattctc
     4561 aacgggcgtg ttgatcgtgc cttgtatgct gacgttggct attcctggag tgctgccgcg
     4621 ctttaaagca gaacagatga tgcccgccat cgcgcttatt gtgtcggtta ttgcctcggt
     4681 agtcattggc ggagcgggga gtctggcgtt cccgctgcct gcattaatct ggtgtgcagt
     4741 gcgctatacg ccgcaggtaa catgtctgtt gacctttgtc accggtgcgg tggaaatcgt
     4801 actggtggct aattcggtga ttgatatctc ggtcggttcg ccgttctcca ttccggaaat
     4861 gttctccgca cgtctcggta ttgccacgat ggcgatatgc ccaattatgg tttcttttag
     4921 cgtggcagcg atcaattcgc taatgaagca agttgcgctg cgagccgact ttgattttct
     4981 gactcaggtt tactcacggt ccggtcttta tgaggcgctg aaaagtccat cgctgaaaca
     5041 gacacaacat ctgactgtca tgctgcttga tatcgactat ttcaaaagta ttaacgataa
     5101 ctatggacat gaatgtggcg ataaagtgtt aagcgtgttt gcccggcata ttcagaagat
     5161 tgtcggtgat aaggggctgg tggcgcgcat gggcggcgaa gaatttgctg ttgcagtgcc
     5221 gtcggtgaat cctgtcgatg gtctgctaat ggcggaaaaa atccgtaaag gcgttgaact
     5281 gcaaccattc acctggcaac aaaaaacgct ttatctcacg gtaagtattg gcgtcggtag
     5341 tggtcgcgca tcgtaccgaa cgctgaccga tgactttaat aaattgatgg tcgaagccga
     5401 tacgtgtctg tatcgctcga agaaagatgg gcgcaaccgt accagcacta tgcgttacgg
     5461 tgaggaagtt gtctgacatc tttgttcatt cagagcgatt taaatgcaat gtgttctacc
     5521 actttatgat ccaggaaaag gcgtttttcc tcgattaaac catgatccgc gccacacttt
     5581 tctcataaaa ctgctcatta atcatcagga gttgatcaaa acaggggttc ggcgtattaa
     5641 agtttgctaa cctgtcgtac caattcaaac aggttgtatg actaatcaga agggaaccca
     5701 ttgtgaaaac actaaatcgt cgcgattttc ccggtgcaca gtatccagaa cgtatcattc
     5761 agtttggtga aggtaacttc ctgcgcgcct ttgttgactg gcaaatcgat ctcctgaatg
     5821 agcacaccga tctgaattct ggtgtggtcg ttgttcgtcc gattgaaact tcattcccgc
     5881 cgtcactgag cacgcaggat ggtctgtaca ccaccattat ccgtggcctg aatgagaagg
     5941 gggaagcggt cagcgacgcg cgtctgattc gctctgttaa tcgtgaaatc agcgtctaca
     6001 gtgaatacga tgaattcctg aaactggcgc acaacccgga aatgcgcttt gttttctcta
     6061 acaccaccga agcgggtatc agctaccatg cgggcgataa attcgatgac gcgccagcgg
     6121 taagttatcc ggcaaaactg acccgtctgc tgttcgaacg ttttagccat ttcaacggtg
     6181 cgctggataa aggttggatc atcatcccgt gtgagttgat cgactacaat ggcgacgcgt
     6241 tgcgtgaact ggtgctgcgc tatgcgcaag agtgggcact gccagaagca tttattcaat
     6301 ggctggatca ggctaacagc ttctgttcta cgctggtgga ccgtatcgtt accggttatc
     6361 cgcgtgatga agtagctaaa ctggaagaag aactgggtta tcacgatggt ttcctcgaca
     6421 ccgctgaaca cttttacctg tttgtgattc agggaccgaa atccttagcg accgagctgc
     6481 gtctggacaa atatccgctc aacgtgctga ttgttgacga tattaaaccg tataaagaac
     6541 gcaaagtggc aatactcaac ggtgcacaca ccgcgttggt gccggtggcg ttccaggcag
     6601 ggctggatac cgtaggtgaa gcgatgaacg atgctgaaat ttgcgcattc gttgaaaaag
     6661 ccatctacga agaaattatt ccggttctgg atttgcctcg cgatgaactg gagtctttcg
     6721 ccagtgcagt caccgggcgt ttccgtaacc cgtacattaa gcatcagctg ctgtctatcg
     6781 cgctcaacgg tatgaccaag ttccgcaccc gcatcctgcc gcagctactg gcagggcaga
     6841 aggcaaacgg cacacttccg gcgcgcctta ctttcgcatt agcggcattg attgcgttct
     6901 atcgtggtga gcgtaacgga gaaacatatc cggtacagga cgatgctcac tggctggaac
     6961 gttatcagca actctggagt cagcatcgtg accgtgttat cggtacccag gaactggtag
     7021 cgattgtgtt ggcagaaaaa gaccactggg agcaggacct gacgcaagtg cctggtctgg
     7081 tcgagcaggt ggctaatgac ctggatgcga ttctcgaaaa agggatgcgc gaagcagtac
     7141 gcccgttgtg ctaatactct gaacccggtt tcgaccgggt
//
LOCUS       DAZKFU020000234         7116 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZKFU020000234 DAZKFU020000000
VERSION     DAZKFU020000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.236"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /locus_tag="ACHOQQ_RS19860"
                     /old_locus_tag="pgaptmp_003972"
     CDS             <1..231
                     /locus_tag="ACHOQQ_RS19860"
                     /old_locus_tag="pgaptmp_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472168.1"
                     /translation="AGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVDQYLV
                     NQSQTVRTSAFGIWQGREEDGVEYQRKLREEW"
     gene            complement(561..1490)
                     /gene="fdhE"
                     /locus_tag="ACHOQQ_RS19865"
                     /old_locus_tag="pgaptmp_003973"
     CDS             complement(561..1490)
                     /gene="fdhE"
                     /locus_tag="ACHOQQ_RS19865"
                     /old_locus_tag="pgaptmp_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGU4472169.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1487..2122)
                     /gene="fdoI"
                     /locus_tag="ACHOQQ_RS19870"
                     /old_locus_tag="pgaptmp_003974"
     CDS             complement(1487..2122)
                     /gene="fdoI"
                     /locus_tag="ACHOQQ_RS19870"
                     /old_locus_tag="pgaptmp_003974"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4472170.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2119..3021)
                     /gene="fdoH"
                     /locus_tag="ACHOQQ_RS19875"
                     /old_locus_tag="pgaptmp_003975"
     CDS             complement(2119..3021)
                     /gene="fdoH"
                     /locus_tag="ACHOQQ_RS19875"
                     /old_locus_tag="pgaptmp_003975"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGU4472171.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3034..6084)
                     /gene="fdnG"
                     /locus_tag="ACHOQQ_RS19880"
                     /old_locus_tag="pgaptmp_003976"
     CDS             complement(3034..6084)
                     /gene="fdnG"
                     /locus_tag="ACHOQQ_RS19880"
                     /old_locus_tag="pgaptmp_003976"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5497..5499),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4472172.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6278..7111
                     /gene="fdhD"
                     /locus_tag="ACHOQQ_RS19885"
                     /old_locus_tag="pgaptmp_003977"
     CDS             6278..7111
                     /gene="fdhD"
                     /locus_tag="ACHOQQ_RS19885"
                     /old_locus_tag="pgaptmp_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGU4472173.1"
                     /translation="MKKTQQKEIENVTNVTGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1577 a         1895 c         1929 g         1715 t
ORIGIN      
        1 gcaggtattg atatgggcag aattatactc gatttatcga atgaggtgat taagcaactt
       61 gatgatcttg aggtgcagcg taatcttcct cgtacggagc tattacggga agcggtagat
      121 caatacttgg taaatcaatc gcaaacagta agaaccagtg cttttggcat atggcaaggg
      181 cgtgaggaag atggtgtcga ataccagcgt aagctgcgcg aggaatggta atgggaaaaa
      241 atgatatcaa tcagattgct gacaacgtgt gcgttgttca tgccggatgc ggcgtgaacg
      301 ccttacccgg cctacaaagt cgtataaatt caatgtattg cagtttattg gtaggcctaa
      361 taagcgcggc gcatcaggca atcttacgct tatcatcagt ctcataccca taaccttatt
      421 gatgtggggg tgggtaaatt gaggcaaaac aggcactggc ggcatagctt gaggtggtaa
      481 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgaccagcgc
      541 cacccggaaa acgtcagaga ttactcccct tcacccggaa acaggaacgg gttgatggaa
      601 ctgcgggcat agccttcttg ctccattcgt gcgtccagta ccagagaggc gaggtcatct
      661 gccacggctt caactttcgg ttctttttcc tgatagagaa ttttcaggta agtgccgcag
      721 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
      781 ttgccgctct gctcacagtt gctgcatttt acgcgcacta cgtgccattc agtttcacac
      841 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
      901 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
      961 ccggggatca gattggccat ctgcgcccag tagagcgaca gtgcagccca gataaacggc
     1021 gctttatcgc tactgacgga tgagaaatca gaggcaaaca gtgcgctggc catatcttcc
     1081 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     1141 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     1201 agaacgtgaa tatccagcgg gggcttgcct tgtgcgctgg cttctttaat gcgtgcagtc
     1261 agatccatct ccagcggatg gtcgtacagc accacttcct gggcgtgggc gataagcgca
     1321 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     1381 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     1441 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     1501 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     1561 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     1621 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     1681 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccac
     1741 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     1801 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     1861 aaaagatatc atcccgattg attaggttgt gatgccagta acggaaaaac atgatgatga
     1921 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     1981 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     2041 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     2101 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     2161 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ctgcgaaggt
     2221 agccgcaaag ccaacagctg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     2281 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     2341 cacgtacata acgtgtgtac caccgacgcc tgccggatcg tacaggcccg cattgtcgta
     2401 accgcgggtt ttcagctccg ccacgcgctc gctcgccagc gttttcatcg actctttcgt
     2461 accgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     2521 cacgcggtca acgcacaacg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     2581 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     2641 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcagaca
     2701 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     2761 ctgctccact tccgagaagc gcatcaccgt ccacgatttg gcgcttaaat cattggggtt
     2821 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     2881 cacctgacag gctttacagc cgatacaggt ggtgacgtcg atgagtttcg ccacttcttc
     2941 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     3001 atcttgcgat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     3061 ggacttaaac tccggcgtct gcgtgtttgc atcaccaacg aatggcgtta acgtattggc
     3121 aataaagcct tttttagcta cgccttcata accccagtga atcggaatac cgatggtatc
     3181 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     3241 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     3301 cgccagcgac tccccgattt ccacaaactg ttctggttgc aaaatcgcgt tcaacagcgc
     3361 gtgtttggtc cagtagtgga aatgctcggt cagacgatag gtggttccga catacgggaa
     3421 cttatcggct ttacccaatg cttcggcgtc gtctttaaag attcgcgcag ccgggttcga
     3481 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     3541 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     3601 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     3661 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     3721 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     3781 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     3841 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     3901 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     3961 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     4021 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctggtccg ggttcgcgcc
     4081 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcgccgtc
     4141 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     4201 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     4261 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     4321 ttcagtgttc agcgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     4381 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     4441 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     4501 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     4561 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     4621 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     4681 catgtaaccc ggcaggctct gcgacagcag ccccaggtcc gtcagcccct gaatattgga
     4741 gtggccgcgc agggcgttaa cgccgccgcc agccatcccc atgttaccga gcagcaactg
     4801 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     4861 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     4921 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     4981 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     5041 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     5101 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     5161 ggcgttggta taggcttcag tgtattcgcg gttgaatttt tcattgttca gcaggtacag
     5221 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     5281 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttagcgc cgttgtgaat
     5341 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     5401 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     5461 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     5521 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     5581 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     5641 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     5701 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     5761 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     5821 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     5881 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     5941 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     6001 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     6061 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     6121 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aagtggccgc
     6181 aaaggtccag aattggctca atttccctcc atccaggaag ggtttgtaac agaataccat
     6241 aatgttggtg tgtgtgttct tatccggtta atagaaagtg aaaaaaacac agcaaaaaga
     6301 aatcgaaaat gtgacaaatg tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     6361 tttacaacac ccacggttag atgaggtggc ggaagaagtt cccgttgcgc tggtctacaa
     6421 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     6481 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     6541 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     6601 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     6661 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     6721 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtgggac aactgactgg
     6781 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     6841 cgtgggtcgc catgtagcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     6901 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     6961 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     7021 agtggctgag cgctgtaatt tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     7081 ttatacccat ccgcagcgtt tgagcaatta attaaa
//
LOCUS       DAZKFU020000235         7075 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZKFU020000235 DAZKFU020000000
VERSION     DAZKFU020000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.237"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(106..996)
                     /gene="metF"
                     /locus_tag="ACHOQQ_RS19890"
                     /old_locus_tag="pgaptmp_003978"
     CDS             complement(106..996)
                     /gene="metF"
                     /locus_tag="ACHOQQ_RS19890"
                     /old_locus_tag="pgaptmp_003978"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGU4472174.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            1072..1317
                     /locus_tag="ACHOQQ_RS19895"
                     /old_locus_tag="pgaptmp_003979"
     CDS             1072..1317
                     /locus_tag="ACHOQQ_RS19895"
                     /old_locus_tag="pgaptmp_003979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472175.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     LTNHVLTASCRIRRKRFIRPNPMIIQYISLETIDPKT"
     gene            complement(1345..3777)
                     /gene="metL"
                     /locus_tag="ACHOQQ_RS19900"
                     /old_locus_tag="pgaptmp_003980"
     CDS             complement(1345..3777)
                     /gene="metL"
                     /locus_tag="ACHOQQ_RS19900"
                     /old_locus_tag="pgaptmp_003980"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGU4472176.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3780..4940)
                     /gene="metB"
                     /locus_tag="ACHOQQ_RS19905"
                     /old_locus_tag="pgaptmp_003981"
     CDS             complement(3780..4940)
                     /gene="metB"
                     /locus_tag="ACHOQQ_RS19905"
                     /old_locus_tag="pgaptmp_003981"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGU4472177.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            5217..5534
                     /gene="metJ"
                     /locus_tag="ACHOQQ_RS19910"
                     /old_locus_tag="pgaptmp_003982"
     CDS             5217..5534
                     /gene="metJ"
                     /locus_tag="ACHOQQ_RS19910"
                     /old_locus_tag="pgaptmp_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGU4472178.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5594..6202
                     /locus_tag="ACHOQQ_RS19915"
                     /old_locus_tag="pgaptmp_003983"
     CDS             5594..6202
                     /locus_tag="ACHOQQ_RS19915"
                     /old_locus_tag="pgaptmp_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGU4472179.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSSPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            6347..7075
                     /locus_tag="ACHOQQ_RS19920"
                     /old_locus_tag="pgaptmp_003984"
                     /pseudo
     CDS             6347..7075
                     /locus_tag="ACHOQQ_RS19920"
                     /old_locus_tag="pgaptmp_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1715 a         1970 c         1795 g         1595 t
ORIGIN      
        1 aaacaaggct gataacctta ttttcccgcc ctcatttcga ggcagcattt tgtgctctgt
       61 ttaaaatttg tgatcactgt gtaattttcg caaaagccac actatttata aaccaggtcg
      121 aacacccagc gtatggcaaa tcgcgtaact catttcagcg cggttaagcg tatagaagtg
      181 gaaatctttc actccttcac ggcttaaaat cttcaccata tccatggcga tattcgcgcc
      241 taccagcttg cgggtttcgg cgtcatcatc caggccgtcg aacatttgcg ccatccacgc
      301 cggaatacgc acgttggtca tatcggcaaa tttcttcgcc tgtttaaagt tagataccgg
      361 caaaattccc ggaataattt ccacatcaat gcccgccgat acacagcggt cacgaaaacg
      421 caggtagctt tcgacatcga agaagaactg agtaatcgcg cggttggctc cggcatcgac
      481 tttgcgtttc agattaagca aatccgcctg agcgcttttt gcttccgggt gaacttccgg
      541 atacgccgcc acggagatat cgaaatctgc cacttctttt aatagcgtca ccaggtcaga
      601 agcatacatt tctggcttac cacttcccgg cggcagatcg ccacgcagcg ccacgatatg
      661 acgaataccg ttattccagt agtcgcgtgc aatggtgcgc aactcgtcgg gcgtcgcatc
      721 aatgcaagta agatgcggtg ccgcttccag accagtgcga tctttaatgc ctttaataat
      781 gctgtgcgta cggtcgcgct cgccggagtt cgcgccatag gtcaccgata caaacttcgg
      841 tttcaggctg ctaaggcgat cgatggagtt ccacagggtc tgctccattt cactggtacg
      901 cggcgggaaa aactcgaacg aaacgttaat ctgcccctgg acttctgcca ggctctgatt
      961 cagggcatcc cgctggctgg cgtgaaaaaa gctcatacct tacctcatca atcgcttgtc
     1021 gttatatgtt gtgttgtgca catctatccg tttagacgtc cagatgtaaa gatgaaggaa
     1081 aagccgaagg gcgtcaaccg aaaaatgacc aatgatggtg aggaaaaatc agggaagatg
     1141 aaaaaacttc aggatggtaa gaaaaagaaa atccggtatg caaagcaaac cggactgcaa
     1201 atattaacta accatgtact tactgcttct tgccggattc gtcgtaaacg cttcatccgg
     1261 cctaatccta tgataattca atatatttca ttagaaacca tagacccgaa aacatgagta
     1321 ccgggcatta ttaaatttct gaaattacaa caactgtgcc agccggttga tatccgactg
     1381 aatcgccccg gcggtgacgt cgcgccccgc gccaggccca cggatcacca gcgggttgtc
     1441 gcgataccag cggctttcga tggcaaagac gttatcgcac ggcagcagtg atgccagcgg
     1501 atggtcttca cgcaccgcct ccacgcccac acgcgctttg ccgttggcat cgaaacgcgc
     1561 cacgtagcgc agcaccagcc ccatttcgcg ggccgcttcc agccgttgca ccatctgctc
     1621 gttaagttca tcgccgtttt caaagaagtg gtcgatgctg ccgccttcgc aatgggcagg
     1681 caccagcgat tccacgcgca cctgatccgg ttcgatgtcg taacccgctt cacgcgccag
     1741 aatcaccagc ttgcgcatca cgtctttgcc ggagaggtcg tcacgcggat caggctcggt
     1801 taagccctgc tgccacgcct gatccaccag ctcggtaaac ggcacgctac cgtcgaattg
     1861 caggaacagc caggagagcg tgccggagaa gatcccgctg atcgacaaaa tagtatcgcc
     1921 gctgtcgatc agatcgcgca cggtgtggtt gatcggcaag cccgcaccga cggtggcgtt
     1981 gtacagccag tgacgcccgg ttttttcgaa ggcgtcgtgg atctggcgat atttattgct
     2041 gtcgctggct cctgccagtt tgttggcgct aatgacgtgg aaaccgtggc tggcgaaatc
     2101 gagatactga tcagcaagct gctggctggc ggtaacgtcc agcaccacta aatcatcata
     2161 cggatgggcg cgcatccaca ggaaaagcga ctcttcatcc tgctcgaccg cttcatcgtt
     2221 gaagaaggct aacgcgcggc tggcgtccag cccgtcataa ctcaacagac tgcggcggct
     2281 gtccaccacg cctgccagca caaattcaaa gccggtacgc gccgaaagcg tgctctgctc
     2341 gcgggcgaac agttccagcc agcgggaacc gatattgcct ttgccgaaca ataccaggcc
     2401 gatgcgtttt tctgcgcgga agacggactg atgcaggccc tgaatcaggc tttcggtcgg
     2461 gccggtgcgc agtactgcca ccaggctgat gccgtcatcg gactgccagg taaactcgac
     2521 cggctggcct ttcagttgct gccagaagcg gtggcaatgc agcgggttac gggtgacacc
     2581 cgccccgacc atcgccacca gcgccagccc ctgacgcagg cgcagttcgc caggtaatcc
     2641 cgcttcgtcg aggattttca gcgcactgtc ggccacttct gaggtgtagc aaaattgcag
     2701 caactggcga tcgttatgca cgccaaccgc cagtggacgc acctgggcac gtttcaggat
     2761 ctggtcgatc tctttatgcg ccagtttgaa atcctggctg gtgggcacct gaaactcaat
     2821 caagcagaca tcatcgtggc tggtgacaat acgcgcacca gtaccggagg ccagcacgcg
     2881 ttcaatgcgc gtggagcctt gatccggcgt gtagctacag cgcagctgca ggtcgatttc
     2941 gctaccggat actggctgta aagtacgggc gtgaagaacg ggagccgcca gacgcgccag
     3001 ttcgctggcc tcatccagac gcagcaacgg cagcagacag gcatctttca ctttacgcgg
     3061 atcggcactg tataccccgg cgacgtcgct ccagatggtt acacgagaaa cacccgccag
     3121 cgcaccgatt tgtgtcgcgg aatagtcgga accgttacgc cccagcagca ccgtttcacc
     3181 ggcgttgtta cggctgataa acccggtcac caccagacgt ttacccggat gttgtaccag
     3241 tagctgttgc agcaacgggt aagaaagccc ttcatcaacc tgcggttgcg cagcgcgttc
     3301 agcgcgtaaa aactcacggg catcaagcca ggccgctggc agcccttgtt gattaagtac
     3361 cgcagacatc aggcgcgccg accacacttc accgtggccc accacttccg catacactgc
     3421 gtcgttaata ccgctgtcga gcagcgccgc caggcgctca agatcgctga caaaagcgct
     3481 aatgaggcta tcggcttctt cggcgggtag caggccgcta atcagatcgc actgatagcg
     3541 acgcagcgtt tgttgaacct gatgcgcaga gagacgatcg gtctggctta gtttcaacca
     3601 gttaatcaac tggttagtgg tgctaccggc ggcggaaacc accatcatat cgtcaggctg
     3661 agagtactcc gccataatgc ccgcgacacg caaataacac ttcacatcag ccagactact
     3721 gccaccaaat ttatgcagct gacgaccttt cgcccctgcc tgcgcaatca cactcatttt
     3781 taccccttgt ttgcagcccg gaagccattt tccaggtcgg caattaaatc ttcgccatct
     3841 tcaataccgg tggagatacg cagcagcgtc tcggagatcc cggcggcagc acgcgcttct
     3901 ggtgccatgc ctgcatgtgt catggttgcg gcgtgagaga ttaaactttc cactccccct
     3961 aatgattccg ccagcgtaaa caacgacagc ccgcccagga aacgacgcag cgtctgctca
     4021 tcgccatcca gttcaaaact caacattgcg ccaaagcctt tttgctgacg cgcggcaatt
     4081 tcatgcccct gattttccgg cagcgacggg tgatacagtt ttttcaccaa cggctgggtt
     4141 tgcagatatt tcacaatcgc ctgcgcgttg cgctgcgcca gctccatacg cggcaccagc
     4201 gttcgcaacc cgcgtagcag cagatagctg tcaaacgcgc cgcccgtcac gccaatattg
     4261 tttgcccacc aggcaagttc agtgacaacg tccgggtctt tagcaatcac cacgccggcc
     4321 accacgtctg agtgaccgtt cagatatttc gtacatgaat gcaacaccag atcggcaccc
     4381 aatgccagcg gattttgtaa tgccgggctt aagaaggtgt tatccaccac gctcaccgcc
     4441 ccgacttccc ttgccagatg gcagattttc gcaatatcca cgacgcgtaa caatggatta
     4501 cttgggcttt ctaccagtac cagtttgggt ttttctgcca gcgctgcccg taatgcctgt
     4561 tcatcgcctt gatcaacaaa caacacgcga tagcaaccgc gtttcgccag gctgtcgaac
     4621 aggcgatagc taccgccgta gcagtcgtgc ggcgcaacca gcagatcgcc aggtttcaaa
     4681 aagacggtcg ttaccaggtg aatcgcggac atgccggtat tagtaagtac tgcaccagca
     4741 ccgccttcca gttctgccag cgcacgctga accacatcgc gcgttgggtt gccgcgacgc
     4801 gagtaatcat gcgcgcgcgg ttcattaaat ccggtaaagt tataggtgct ggaaagatgg
     4861 atgggaggga caacgcaacc atactgttcg tcgtcattta acccgctacg cactgcgatg
     4921 gtggcctgtt tacgcgtcat gtgatgaagt tccctaggct tggtcggtga aatgtcaggc
     4981 accagagtaa acattgtgtt aatggacgtc aatacatctg gacatctaaa cttctttgcg
     5041 tatagattga gcaaatccca aatagccgtt aaaattatat gcattatcac gccgacaggt
     5101 gcattacacg atgtcacggt aacgcctgta cggtaaacta tgcgggttta cggtcagtac
     5161 ccacataaat tgtgtggtct ggtctcaatt tattgacgaa gaggattaag tatctcatgg
     5221 ctgaatggag cggcgaatat atcagcccat acgctgagca cggcaagaag agtgaacaag
     5281 tcaaaaagat tacggtttcc attcctctta aggtgttaaa aatcctcacc gatgaacgca
     5341 cgcgtcgtca ggtgaacaac ctgcgtcacg ctaccaacag cgagctgctg tgcgaagcgt
     5401 ttctgcatgc ctttaccggg caacctttgc cggatgatgc cgatctgcgt aaagagcgca
     5461 gcgacgaaat cccggaagcg gcaaaagaga tcatgcgtga gatggggatt aacccggaga
     5521 cgtgggaata ctaaagcgga aaagagcggc gcggagttaa ccagccgctt ttttcataca
     5581 aggacgttta acgatgaaaa tcaggctact gatcctcagc ctgctggttt ctgtgcctgc
     5641 ctttgcctgg cagccgcaaa ctggcgacat catctttcaa atctctcgct catcgcaaag
     5701 taaagcgatc caactggcga cccattccga ttacagccac accggtatgc tggtgatgcg
     5761 taacaaaaag ccctacgttt ttgaagcggt cggcccggtg aaatacaccc cgctcaagca
     5821 gtggattgcc catggtgaaa agggcaaata cgttgttcgc cgcgttgaag gcggactcag
     5881 tgttgaacaa cagcaaaaac tggcgcaaac ggcgaaacgt tatctcggca aaccgtatga
     5941 cttcagcttc tcatggagcg acgatcgcca gtactgttcg gaagtggtgt ggaaggttta
     6001 tcagaatgcg ctgggaatgc gcgtgggcga acagcagaag ttgaaagagt tcgacctgag
     6061 cagcccacaa gttcaggcaa agctcaaaga acgttacggc aagaatattc cgcttgagga
     6121 aacggtggtc tcgccacaag ccgttttcga tgcgccacaa ctcaccacgg tcgccaaaga
     6181 atggccgctg ttttcgtggt aatggatgat aaaaaaaata attcccgccg tagtctgttt
     6241 gttgctttgt agttgcacga cctttccgca agctgtcgct ccggtaaacg gtgatgcctc
     6301 caattagttg aattaaggat ataatactcg cttttaaggt tgtttcatgg tcacagttaa
     6361 ccttcattgt ccccgttgcc agtccgttca ggtttaccgt cacgggcaga accccaaaag
     6421 acatgatcga ttccgcggcc gtgactgtca ccgggtattt cagctcagtt actgttacga
     6481 gccccgtaaa ccgggagtta aagaccaaat cgctgaaatg gctttcaacg gtgccggggt
     6541 tcgctatact gcccgtacgc tgaaaatcgg cattaataca gtcatccgaa ctttaaaaaa
     6601 ctctcgccac gaagaataac gtcatccccg gtcgctcatg ctgatgtggc actcatctgc
     6661 gaacttagtc agcaatggag ctttgtcggc agtaaagcgc gacagcactg gctctggtac
     6721 gtgtataaca cgaaaactgg tggtgttctg gcctacactt ttggcccccg gactgatgaa
     6781 acctgtcgtg aattgctggc gctgcttacc cttttaccat cggcatgttg accactgacg
     6841 aatggggtag ttacgctaga gagttaccaa aggagaagca tctgactggt aagattttta
     6901 ctcagcacat tgagcgtaac aatctgaccc tgagaacccg aatcaaacgt ctggcccgca
     6961 gaaccatctg tttctcgcgt tctgtagaac ttcatgaaaa agtcatcggt gcttttatcg
     7021 aaaatatatg ttctactaat tggagtcatc acccttctga tgagctttcc ttgga
//
LOCUS       DAZKFU020000236         7069 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZKFU020000236 DAZKFU020000000
VERSION     DAZKFU020000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.238"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            115..1512
                     /gene="ulaA"
                     /locus_tag="ACHOQQ_RS19925"
                     /old_locus_tag="pgaptmp_003985"
     CDS             115..1512
                     /gene="ulaA"
                     /locus_tag="ACHOQQ_RS19925"
                     /old_locus_tag="pgaptmp_003985"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU4472180.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1528..1833
                     /gene="ulaB"
                     /locus_tag="ACHOQQ_RS19930"
                     /old_locus_tag="pgaptmp_003986"
     CDS             1528..1833
                     /gene="ulaB"
                     /locus_tag="ACHOQQ_RS19930"
                     /old_locus_tag="pgaptmp_003986"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGU4472181.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQYLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1843..2307
                     /gene="ulaC"
                     /locus_tag="ACHOQQ_RS19935"
                     /old_locus_tag="pgaptmp_003987"
     CDS             1843..2307
                     /gene="ulaC"
                     /locus_tag="ACHOQQ_RS19935"
                     /old_locus_tag="pgaptmp_003987"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGU4472182.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2321..2971
                     /gene="ulaD"
                     /locus_tag="ACHOQQ_RS19940"
                     /old_locus_tag="pgaptmp_003988"
     CDS             2321..2971
                     /gene="ulaD"
                     /locus_tag="ACHOQQ_RS19940"
                     /old_locus_tag="pgaptmp_003988"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4472183.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2981..3835
                     /gene="ulaE"
                     /locus_tag="ACHOQQ_RS19945"
                     /old_locus_tag="pgaptmp_003989"
     CDS             2981..3835
                     /gene="ulaE"
                     /locus_tag="ACHOQQ_RS19945"
                     /old_locus_tag="pgaptmp_003989"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGU4472184.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3835..4521
                     /gene="ulaF"
                     /locus_tag="ACHOQQ_RS19950"
                     /old_locus_tag="pgaptmp_003990"
     CDS             3835..4521
                     /gene="ulaF"
                     /locus_tag="ACHOQQ_RS19950"
                     /old_locus_tag="pgaptmp_003990"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGU4472185.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4650..4925)
                     /gene="yjfY"
                     /locus_tag="ACHOQQ_RS19955"
                     /old_locus_tag="pgaptmp_003991"
     CDS             complement(4650..4925)
                     /gene="yjfY"
                     /locus_tag="ACHOQQ_RS19955"
                     /old_locus_tag="pgaptmp_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGU4472186.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5252..5647
                     /gene="rpsF"
                     /locus_tag="ACHOQQ_RS19960"
                     /old_locus_tag="pgaptmp_003992"
     CDS             5252..5647
                     /gene="rpsF"
                     /locus_tag="ACHOQQ_RS19960"
                     /old_locus_tag="pgaptmp_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGU4472187.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5654..5968
                     /gene="priB"
                     /locus_tag="ACHOQQ_RS19965"
                     /old_locus_tag="pgaptmp_003993"
     CDS             5654..5968
                     /gene="priB"
                     /locus_tag="ACHOQQ_RS19965"
                     /old_locus_tag="pgaptmp_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGU4472188.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5973..6200
                     /gene="rpsR"
                     /locus_tag="ACHOQQ_RS19970"
                     /old_locus_tag="pgaptmp_003994"
     CDS             5973..6200
                     /gene="rpsR"
                     /locus_tag="ACHOQQ_RS19970"
                     /old_locus_tag="pgaptmp_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGU4472189.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6242..6691
                     /gene="rplI"
                     /locus_tag="ACHOQQ_RS19975"
                     /old_locus_tag="pgaptmp_003995"
     CDS             6242..6691
                     /gene="rplI"
                     /locus_tag="ACHOQQ_RS19975"
                     /old_locus_tag="pgaptmp_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGU4472190.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6762..>7069)
                     /locus_tag="ACHOQQ_RS19980"
                     /old_locus_tag="pgaptmp_003996"
     CDS             complement(6762..>7069)
                     /locus_tag="ACHOQQ_RS19980"
                     /old_locus_tag="pgaptmp_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU4472191.1"
                     /translation="NCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHD
                     LKIGLRSLSSSAAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         1681 a         1753 c         1956 g         1679 t
ORIGIN      
        1 cgcgtcacat ttaatcataa ataatcttgt tgtgattact tttgaaaatt agagtgagtg
       61 cacaacattc cgggtgtgag gaatacccgg ttacctcttc ttcaggagat cgttatggag
      121 atcctctaca acatctttac cgtgtttttt aaccaggtca tgaccaatgc cccgttgttg
      181 ctgggtattg tgacctgtct gggctacatc ctactgcgca aaagtgtcag cgttattatt
      241 aaaggcacga ttaaaaccat aattggtttc atgttgttgc aggcagggtc cggcatcctc
      301 accagcacct tcaaaccggt ggtggcgaaa atgtccgaag tctacggcat taacggcgca
      361 atttcagata cttacgcttc gatgatggca accatcgacc gcatgggcga tgcctatagc
      421 tgggtgggtt acgccgtatt gttagcgctg gcgctgaaca tctgttacgt gctgctgcgt
      481 cgcattaccg gcattcgcac aatcatgttg accggccaca tcatgttcca gcaggccggg
      541 ttgattgccg ttacgctgtt tatcttcggc tactccatgt ggaccaccat tatctgtacc
      601 gcgattctgg tttcgctcta ctggggcatc acttccaaca tgatgtacaa gccgactcag
      661 gaagtgacgg atggctgtgg tttctccatc ggtcaccagc agcagtttgc atcatggatt
      721 gcctataaag tcgcaccgtt cctcggcaaa aaagaggaga gcgttgaaga cctcaaattg
      781 ccgggctggc tgaacatttt ccacgacaac atcgtctcca cggcgattgt gatgaccatc
      841 ttctttggcg ccattctgct ctccttcggt atcgacaccg tgcaggcgat ggcgggcaaa
      901 gtgcactgga cggtctacat cctgcaaacc ggtttctcct tcgcggtggc gatcttcatc
      961 atcactcagg gcgtgcgcat gtttgtggcg gaactctctg aagcatttaa cggtatctcc
     1021 cagcgcctga tcccaggtgc ggttctggcg attgactgtg cggctatcta tagcttcgcg
     1081 ccgaacgccg tggtctgggg ctttatgtgg ggcaccatcg gtcagctgat tgcagttggc
     1141 atcctggtcg cctgcggctc ttccattctg attattctag gctttatccc gatgttcttc
     1201 tccaacgcca ccatcggcgt gttcgctaac cacttcggcg gctggcgtgc agcgctgaag
     1261 atttgtctgg tgatgggaat gatcgaaatc tttggttgcg tctgggcggt gaaactcacc
     1321 ggtatgagtg cctggatggg catggcggac tggtcgattc tggcaccgcc gatgatgcaa
     1381 ggcttcttct ccatcggtat cgcctttatg gccgtcatca ttgtaattgc actggcttat
     1441 atgttcttcg ctggccgcgc actgcgcgca gaagaagatg cagaaaaaca actggcagaa
     1501 cagtctgctt aataaggagt tttgattatg accgtacgta ttctggctgt gtgtggcaac
     1561 ggacaaggca gttccatgat catgaaaatg aaagtggacc agtatttaac ccaatcaaac
     1621 attgaccaca cggtaaacag ctgcgcggtt ggcgagtaca aaagcgagtt gagcggcgcg
     1681 gatatcatca tcgcttctac gcacatcgcg ggcgaaatca ccgttaccgg caacaaatat
     1741 gtggttggcg tgcgcaacat gctctctcct gccgactttg gcccgaaact gctggaagtg
     1801 atcaaagagc atttcccgca ggacgtgaag taaggatacg ccatgaaact acgtgattcg
     1861 ctggcggaga ataaatctat ccgcctgcag gctgaagcag agacatggca ggacgcagta
     1921 aaaatcggcg ttgacctgct ggtggcggcg gatgttgtcg agccgcgtta ctaccaggcg
     1981 attctggatg gcgtggaaca gtttggccct tatttcgtta tcgctccggg gctggcgatg
     2041 ccgcacgggc ggccggaaga gggtgttaag aaaacaggtt tctctctggt aacgctgaaa
     2101 aagccgctgg aatttaatca cgacgacaac gatccggtgg atatcctcat caccatggcg
     2161 gcggtcgatg ccaatactca ccaggaagtg ggcatcatgc agatcgtcaa cctgtttgaa
     2221 gatgaagaga attttgaccg tttacgcgcc tgccgtaccg agcaggaagt actggatctc
     2281 attgaccgca ccaacgcggc agcttaagaa ggaattgaac atgtcattac cgatgttgca
     2341 agtcgcgctg gacaaccaga ctatggatag cgcctacgaa accactcgcc tgattgccga
     2401 agaagtcgac attatcgaag tgggtaccat tctgtgcgtg ggcgaaggcg tgcgtgcggt
     2461 tcgcgatctg aaagcgcttt acccgcacaa aatcgtactg gcagacgcca aaattgccga
     2521 tgcgggcaaa atcctttcgc gtatgtgctt cgaagccaac gctgactggg tgacggtaat
     2581 ttgctgtgcg gatatcaaca ccgccaaagg cgcgctggac gtggcaaaag agtttaacgg
     2641 cgacgtgcag atcgaactga ccggttactg gacctgggaa caggcgcaac agtggcgcga
     2701 tgcaggcatt cagcaggtgg tttatcaccg cagccgtgac gcgcaggccg caggcgtggc
     2761 gtggggcgaa gcggacatca ccgcgatcaa acgtctttcc gatatgggct tcaaagtcac
     2821 cgtcaccgga ggcctggcgc tggaagatct gccgctgttc aagggtattc cgattcacgt
     2881 ctttatcgcg ggccgtagta tccgtgatgc cgcttctccg gtggaagccg cacgtcagtt
     2941 caaacgttcc atcgctgaac tgtggggcta aggagcggat atgttgtcca aacaaatccc
     3001 gcttggcatc tatgaaaaag cgctccccgc cggggagtgc tggctggaac gcctgcaact
     3061 ggcaaaaacg ttgggcttcg attttgtcga gatgtcggta gatgaaacgg acgagcgcct
     3121 gtcgcgcctc gactggagcc gcgagcagcg tctggcgctg gtcaatgcga ttgttgaaac
     3181 cggcgtgcgc gtgccgtcca tgtgcctttc tgctcatcgt cgtttcccgc tgggcagtga
     3241 agatgacgcg gtgcgggcgc aggggctgga gattatgcgt aaagctatcc agttcgccca
     3301 ggatgtcggt attcgcgtga tccagctggc gggctatgac gtttactatc aggaagccaa
     3361 taacgaaacg cgtcgtcgtt tccgtgacgg cctgaaagag agcgttgaga tggcaagccg
     3421 cgcgcaggtg acgctggcga tggagatcat ggattatccg ttgatgaact ccatcagcaa
     3481 ggcgctgggc tacgcgcact atctcaacaa tccgtggttc cagctctacc cggatatcgg
     3541 caacctgtcg gcgtgggata acgacgtgca gatggagttg caggccggaa tcgggcatat
     3601 cgtcgcggtg catgtgaaag acaccaaacc gggcgtcttc aaaaacgtgc cgtttggcga
     3661 aggtgtagtg gatttcgaac gttgtttcga aacgctcaaa cagagtggct attgcgggcc
     3721 gtacctgatt gagatgtgga gcgaaacggc ggaagacccg gcggcagaag tggcgaaagc
     3781 gcgtgattgg gtgaaagcgc gcatggcgaa agcgggcatg gtggaggcgg cataatgcaa
     3841 aagctaaaac agcaggtatt tgaagccaac atggagctgc cgcgctacgg gctggtgacc
     3901 tttacctggg gcaacgtcag cgctatcgac cgcgaacgcg ggctggtggt gatcaagccc
     3961 agcggcgttg cctacgaaac catgaaagcg gccgatatgg tggtggttga tatgagcggc
     4021 aaggtggtgg aaggggagta tcgcccatct tccgacactg cgacgcatct cgaactctac
     4081 cgtcgttacc cgtcgcttgg cggcattgtc catacccact ccactcatgc caccgcatgg
     4141 gcgcaggcgg ggctggcgat cccggcgtta ggcaccacgc acgctgacta cttctttggc
     4201 gacattccgt gtacgcgcgg gttaagcgaa gaagaggtgc agggcgagta tgaactgaac
     4261 accggcaaag tgattatcga aacgctgggc aacgccgagc cgctgcatac gccgggaatt
     4321 gtggtgtatc agcacggacc gttcgcctgg gggaaagatg cccacgatgc ggtgcataac
     4381 gcggtggtga tggaagaagt ggcgaaaatg gcgtggattg cgcgcggcat taacccacaa
     4441 ctgaatcaca tcgacagctt cctgatgaat aaacacttca tgcgtaaaca cggtcctaac
     4501 gcttattacg ggcagaagta gaacacgcgc tgcggaaatt tccttcctcg ggagataact
     4561 ggtctaattc cgcagccgtt tttcaaaaaa agccccctgc gaagggggca aagcaaacta
     4621 tggcaatgtt tcgttggtta tacctggtgc tagcgataaa tatccgcgct ggcgtgcata
     4681 ttgccgttac tccccggttc ccgcatcaga attacgtggt agtacgttgc gccttgcgca
     4741 tctgtttctt catttaatgc ctgacgtgct tcgctttcag tggcgaaatt atgattgata
     4801 taaatcacgc ctaagctttg cacatcgtcc atatttctgg cctggtggtt attaatttca
     4861 atggctgccc atgtatttgc acttagcaaa agcacagcca gaagggctaa aacacgactg
     4921 aacatagata cctcctcgac ggctgacttt gtgtgctctc ctctgtgatg atcttctgat
     4981 ttaattttaa tcaatgataa agaagttgat ggtgaccatt tctgatgcag ttgttcaaaa
     5041 aaacaccatg atgaagtgtg atgaacttca aatcagcgtg ttagtccact ttggtgaaag
     5101 atgagattta tttgtcggct ggccttgagt ttagcggggt atgtcagtac tataacgcgt
     5161 catttttcag ccgaccttta acacgttcct tgcctccccg ggattcggct gacccagaca
     5221 ggaggctgaa taatccgtaa ggagcaattc gatgcgtcat tacgaaatcg tttttatggt
     5281 ccatcctgac cagagcgaac aggttccggg catgatcgag cgctacactg ctgccatcac
     5341 tggtgcagaa ggcaagatcc accgtctgga agactggggc cgccgtcagc tggcttaccc
     5401 gatcaacaaa ctgcacaaag ctcactacgt tctgatgaac gttgaagctc cgcaggaagt
     5461 gatcgatgag ctggaaacta ccttccgctt caacgatgcc gttatccgca gcatggttat
     5521 gcgtaccaag cacgctgtta ccgaagcatc tccgatggtt aaagcgaaag acgagcgccg
     5581 tgagcgtcgc gatgatttcg caaacgaaac cgctgatgat gctgaagctg gggattctga
     5641 agagtaattt ctgatgacca accgtctggt gttgtccggc accgtgtgca ggactcccct
     5701 tcgaaaggtc agtccatcag gaattcctca ctgccagttc gtgcttgagc atcgttctgt
     5761 gcaggaggaa gccggctttc accggcaggc atggtgtcaa atgcccgtta ttgttagcgg
     5821 acacgaaaac caggccatta ctcacagtat aacggtcggc agtcgcataa ccgttcaggg
     5881 gttcatttca tgccacaagg caaagaacgg actgagcaaa atggttttgc atgccgagca
     5941 gattgaattg atagattctg gagactagcc atatggcacg ttatttccgt cgtcgcaagt
     6001 tctgccgttt caccgcggaa ggcgttcaag agatcgacta taaagatatc gctacgctga
     6061 aaaactacat caccgaaagc ggtaagattg tcccaagccg tatcaccggt acccgtgcaa
     6121 aataccagcg tcagctggct cgcgctatca aacgcgctcg ctacctgtcc ctgctgccgt
     6181 acactgatcg ccatcagtaa tcggtcacgg tccattaata cgactttgag aggataaggt
     6241 aatgcaagtt attctgcttg ataaagtagc aaacctgggt agcctgggtg atcaggtaaa
     6301 cgttaaagcg ggctatgctc gtaacttcct ggtaccgcag ggtaaagctg ttccagctac
     6361 caagaaaaac attgaattct tcgaagcacg tcgcgctgaa ctggaagcta aactggctga
     6421 agttctggca gcagctaatg ctcgcgctga gaaaatcaat gcactggaaa ctgttaccat
     6481 cgcgtctaaa gctggcgacg aaggtaaact gttcggttcc atcggtactc gcgacatcgc
     6541 tgacgctgta actgcagctg gcgttgaagt ggctaagagc gaagttcgtc tgccgaacgg
     6601 cgttctgcgt accactggcg aacacgaagt gagcttccag gttcacagcg aagtattcgc
     6661 gaaagtgatc gtaaacgtag tagctgaata attcgttatt caacgagacg taaaaacgcc
     6721 gaccattggt cggcgttttg ctttctattt ttcgtcaggt attagtttcg caagtagatc
     6781 cattcaccat tctcttgtgt ccataagtca ggatggtttg ctggcgttag atacatacga
     6841 gcgcaactga tgtctcgctc ctgcggcact cgctcgaaac ccaaacccga gtagattgcg
     6901 gctgaacttg ataaggaacg cagaccaatt ttcaaatcat ggggtaaatc agaatacgcg
     6961 tttagcaatg cagcaatgat gaatccgagg gcattagaaa cgcctgtaac agtgcgaagt
     7021 tggtcgatat aaagatcgat tgttggtagt tcagatgacc agcaattag
//
LOCUS       DAZKFU020000237         7052 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZKFU020000237 DAZKFU020000000
VERSION     DAZKFU020000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.239"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            206..1081
                     /gene="rluB"
                     /locus_tag="ACHOQQ_RS19985"
                     /old_locus_tag="pgaptmp_003997"
     CDS             206..1081
                     /gene="rluB"
                     /locus_tag="ACHOQQ_RS19985"
                     /old_locus_tag="pgaptmp_003997"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGU4472192.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(1121..1711)
                     /gene="cobO"
                     /locus_tag="ACHOQQ_RS19990"
                     /old_locus_tag="pgaptmp_003998"
     CDS             complement(1121..1711)
                     /gene="cobO"
                     /locus_tag="ACHOQQ_RS19990"
                     /old_locus_tag="pgaptmp_003998"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU4472193.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(1708..2466)
                     /locus_tag="ACHOQQ_RS19995"
                     /old_locus_tag="pgaptmp_003999"
     CDS             complement(1708..2466)
                     /locus_tag="ACHOQQ_RS19995"
                     /old_locus_tag="pgaptmp_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGU4472194.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2686..3735
                     /gene="sohB"
                     /locus_tag="ACHOQQ_RS20000"
                     /old_locus_tag="pgaptmp_004000"
     CDS             2686..3735
                     /gene="sohB"
                     /locus_tag="ACHOQQ_RS20000"
                     /old_locus_tag="pgaptmp_004000"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGU4472195.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3771..4022)
                     /locus_tag="ACHOQQ_RS20005"
                     /old_locus_tag="pgaptmp_004001"
     CDS             complement(3771..4022)
                     /locus_tag="ACHOQQ_RS20005"
                     /old_locus_tag="pgaptmp_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGU4472196.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4402..6999
                     /gene="topA"
                     /locus_tag="ACHOQQ_RS20010"
                     /old_locus_tag="pgaptmp_004002"
     CDS             4402..6999
                     /gene="topA"
                     /locus_tag="ACHOQQ_RS20010"
                     /old_locus_tag="pgaptmp_004002"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGU4472197.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKHQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1794 a         1678 c         1914 g         1666 t
ORIGIN      
        1 gatgttaatc gaaacaagct ttaaccataa agatagtgta gtatgttgcg cctcaaagct
       61 aggccagtta aaacgtcgag tcgtttactt aaggcctgaa gagttcaaac agactttact
      121 atcagtgagt cagatgtaag ttctgactca ctgttgcaca aaagcatatc agacgcctgg
      181 gaaggcgaca ccaaaaggaa gctctatgag cgaaaagcta cagaaagtgc tggcgcgtgc
      241 cggccacggt tctcgccgtg aaatcgaatc tattattgaa gccggtcgtg tgagtgttga
      301 tggcaaaatt gctaaactcg gcgatcgcgt tgaagttacc cctggattga aaatccgtat
      361 cgatggtcac ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta
      421 ttacaaaccg gaaggtgagt tgtgtacccg taacgatccg gaaggacgtc caacggtgtt
      481 tgaccgtctg ccaaaactgc gtggcgcacg ctggattgcc gtgggtcgct tggacgttaa
      541 tacctgtggt ctgctgctgt tcaccaccga tggtgaactg gcaaaccgtt taatgcaccc
      601 aagccgtgaa gttgaacgtg aatatgccgt gcgtgtattt ggtcaggttg acgacgcgaa
      661 actgcgtgat ttgagtcgtg gcgtgcagtt ggaagatggt ccggcagctt ttaaaaccat
      721 caagttcagc ggcggcgaag ggatcaacca gtggtacaac gtgactctga ccgaaggccg
      781 taaccgtgaa gttcgtcgtc tgtgggaagc ggttggtgtg caggtgagcc gcctgatccg
      841 tgttcgttac ggtgatatcc cactgccaaa aggtctgcct cgcggtggct ggacggagct
      901 ggacctcgcc cagactaact atctgcgcga actggtggag ctaccgccag aaaccagctc
      961 taaagtcgct gtagaaaaag accgtcgtcg catgaaggcg aatcagattc gtcgtgcggt
     1021 gaaacgtcac agtcaggtga gcggcggtcg tcgttctggc ggacgtaata ataacggtta
     1081 atcaaagtat gccggacgtc atatccggca tttttacaga ttaataatcg atccctatct
     1141 gcgcttttac accggcatca aacgcatgtt tgatggggcg caactcactc accgtatccg
     1201 ccagttcaag aatatcccga tgacagcctc gacccgtgat aatcaccgtc tgttgatgtg
     1261 gacgttcatt taacgcctgc aacacttctt ccagtggcaa atagtcatac gccaccatat
     1321 atgtcagctc atcgagcaaa accatatcca gtgaggaatc agcaagcatc cgctttgcat
     1381 gttgccagac ttcgcggcag gcggcggtat cagactcgcg gttttgtgta tcccaggtaa
     1441 agcccgttgc catcacctga aactcaacgc catgtggctc cagcagattg cgttcgccat
     1501 taggccaggt gcctttaata aactgcacga cgcccacttt ttttccgtga ccaactgcgc
     1561 gtgttgccgt accaaatgcc gcggtggttt tgccttttcc attgccggta aagacgatga
     1621 taataccgcg ttcatcctgg gcctgggcca cacgagcatc tactttttct ttcactcgct
     1681 gctgacgctg ttggtagcgt tcatcactca ttgggaaatt cctggtttac ggcccggttg
     1741 ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc agaggtagag
     1801 cggcatgata tcagcgggag ttttaagttt ctgtggatct tcggtcggga aggcgctggc
     1861 acgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg acgcgcaggc gctgctgata
     1921 ttcatcggcc agtacctgca tcatcccttc ggtggcaaat ttcgacgctg catatgcgcc
     1981 ccagttggct cgtccctgac gtccaacgct tgatgaagta aagaccagtg aaccggcgtc
     2041 cgatttgagt aataaaggaa gcagtgcctg ggtgagcata aaggtggcat taacgttgac
     2101 ctgtatgacg tcctgccaga cctgtggatt ttgttcgctc attggacaaa catcgccgag
     2161 caatccggca ttatgcaaaa caccatccag acgcggataa ttaacggcaa tgcgctgtgc
     2221 cagttgttgg caatcttcgg acctgcaggt cagcaaatcg agaataaacc actgtggctg
     2281 acgcccagtt tcttcgttta tgtggctggc tacctgacgt aatttttctt cattacggcc
     2341 caacagaatc actgtcgcac catagcgtgc atacgtcatc gcggcttcac gaccaatacc
     2401 atcactggct cccgtcacca ggataatgcg atcattgagt aaatcttgtt ttggctggta
     2461 atgcatggct actcctcaac gacgttgtct gtcgcgctgc cagtgtactt tatgactttg
     2521 gggctttatg cctgaaacag aagggcattt caatcagcct ggggcgataa caccgcaaaa
     2581 tggatacttt gtcatacttt cgctgcaata acatctctgc gagacggctt aacatgcctg
     2641 ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga attgttgtct
     2701 gaatatggtt tgtttttggc gaaaatcgtt accgttgtgc tagcgattgc ggcgattgcc
     2761 gccattattg tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt acgggtcaac
     2821 aatctcagcg aacagtataa ggagatgaaa gaagaactgg ccgcggcgct gatggactca
     2881 catcagcaaa aacagtggca caaagcgcag aagaaaaagc ataagcaaga agcgaaagca
     2941 gcaaaagcga aagccaaact gggcgaggtg gcaactgaca gtaaaccccg cgtctgggtg
     3001 ctggatttta aaggcagcat ggacgcccat gaagtgaact cgctacgtga agagataacg
     3061 gctgtactcg cagcattcaa accgcaggat caggttgtgc tacgtctgga aagccctggt
     3121 ggcatggtgc atggttacgg gttggcggct tcgcagctgc agcgtctgcg tgataaaaac
     3181 attcctttaa ctgttacggt agacaaagtc gctgccagcg gcggttacat gatggcctgt
     3241 gtggcggaca aaattgtttc cgcaccgttt gctattgtgg gttccattgg tgtggtggcg
     3301 caaatgccca actttaaccg cttcctgaaa agcaaagata ttgatatcga actgcacacc
     3361 gccgggcagt ataagcgcac gctgaccttg ctgggtgaaa ataccgaaga agggcgggag
     3421 aaattccgcg aagagctgaa cgaaacgcat cagttattta aagattttgt gaagcgtatg
     3481 cgtccgtctc tggatattga acaggtggca acgggtgaac actggtacgg acaacaggcg
     3541 gtagagaaag gcctggttga tgaaatcaac accagtgatg aagttattct tagcctgatg
     3601 gaaggccgtg aagtggtcaa tgtacgctat atgcagcgta aacgactcat tgaccgattc
     3661 accggcagcg cggcagagag cgccgataga ttattgctac gctggtggca gcgtgggcaa
     3721 aagccattga tgtaaaagac aaacgcgagg gtaagacctc gcgttttgct ttaatcaacc
     3781 agatgatatt tttctgaaag cacatgggcc aggtgtttga acatattaaa caccgcggtg
     3841 cttttggctg ttggcaatcc ttgttcatct aaaaagtagt cgccggtaaa taccagcacg
     3901 ccattacgct gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc atgctcacga
     3961 atgattttgt tggcctcttt caacagcgtt tcgcggtcga tgggttgtgt ctctttgttc
     4021 attatttact ccttaaacaa ggacattagt ctacgccagg catggcttgc agacaaatat
     4081 accacgctgg tggcaagagc gccttactgg caactttgga ttttgcatgc taataaagtt
     4141 gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc aatctggcaa tagatttgct
     4201 tgacattcga ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc tgttaactca
     4261 gtcacctgaa ttttcgtgaa cagagtcacg acaaggggtt gatatccgca gagagcgagt
     4321 ccatatcggt aactcgttgc cagtggaagg tttatcaacg tgcgacgcat tcctggaaga
     4381 atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc cccggcaaaa
     4441 gccaaaacga tcaacaagta tctgggtagt gactacgtgg ttaaatccag cgtcggtcac
     4501 atccgcgatt tgccgaccag tggctcagct gccaaaaaga gtgccgactc tacctccacc
     4561 aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg tatgggggtt
     4621 gacccgtggc acaattggga ggcgcactat gaagtgttgc ctggtaaaga gaaggtcgtc
     4681 tctgaactga aacaactggc tgaaaaagcc gaccacatct atctcgcaac cgaccttgac
     4741 cgcgaagggg aagccattgc atggcacctg cgggaagtga ttggtggtga tgatgcgcgc
     4801 tatagccgag tggtgtttaa cgaaattact aaaaacgcga tccgccaggc gtttaacaaa
     4861 ccgggtgagc tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt tatggaccgc
     4921 gtggtggggt atatggtttc gccgctgcta tggaaaaaga tcgctcgtgg tctgtctgcc
     4981 ggtcgtgtgc agtcggtggc ggttcgcctg gtggtcgagc gtgagcgtga aattaaagcg
     5041 ttcgtgccgg aagagttctg ggaagtcgat gccagcacga ccacgccatc tggtgaagcg
     5101 ttggcgttgc aggtgactca tcagaacgac aaaccgttcc gtccggtcaa caaagaacaa
     5161 actcaggctg cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga acgtgaagac
     5221 aaaccgacaa ccagtaaacc tggcgctcct tttattacct ctacgctgca acaagctgcc
     5281 agcacccgtc ttggatttgg cgtgaaaaaa accatgatga tggcgcagcg tttgtatgaa
     5341 gcaggctata tcacttacat gcgtaccgac tccactaacc tgagtcagga cgcggtaaat
     5401 atggttcgcg gttatatcag cgataatttt ggtaagaaat atctgccgga aagtccgaat
     5461 cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag cgattcgtcc ttctgacgtc
     5521 aatgtgatgg cggaatcgct gaaggatatg gaagcagatg cgcagaaact gtaccagtta
     5581 atctggcgtc agttcgttgc ctgccagatg accccagcga aatatgactc cacgacgctg
     5641 accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg ctttgatggc
     5701 tggacaaaag tgatgcctgc actgcgtaaa ggcgatgaag atcgtatctt accggcagtc
     5761 gataaaggcg atgctctgac gctcgttgaa ctgacaccag cccagcactt taccaagccg
     5821 ccagcccgtt tcagtgaagc atcgctggtt aaagaactgg aaaaacgcgg tatcggtcgt
     5881 ccgtctacct atgcgtcgat catttcgacc attcaggatc gtggctatgt gcgtgtagaa
     5941 aaccgtcgtt tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct ggaagagaat
     6001 ttccgcgagt taatgaacta cgacttcacc gcgcagatgg aaaacagcct cgaccaggtg
     6061 gcaaatcacg aagcagagtg gaaagctgta ctggatcact tcttctcgga tttcactcag
     6121 cagttagata aagctgaaaa agatccggaa gaggggggta tgcgtccgaa ccagatggtt
     6181 ctgaccagca tcgactgccc gacctgtggt cgcaaaatgg ggattcgcac agcgagcacc
     6241 ggggtattcc ttggctgttc tggctatgcg ctgccgccga aagagcgttg caaaacaacc
     6301 attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg aaggcgaaga cgctgaaacc
     6361 aacgcgctgc gcgcaaaacg tcgttgcccg aaatgcggca cggcgatgga cagctatctc
     6421 atcgatccga aacgtaagtt gcatgtctgt ggtaataacc caacctgcga cggttacgag
     6481 atcgaagagg gcgaattccg cattaaaggt tatgacggcc cgatcgttga gtgtgaaaaa
     6541 tgtggttctg aaatgcacct gaaaatgggg cgtttcggta aatatatggc ctgcaccaac
     6601 gaagagtgta aaaacacgcg taagatttta cgtaacggcg aagtggctcc accgaaagaa
     6661 gatccggtac cattaccgga gctgccgtgc gaaaaatcag atgcctattt cgtgctgcgt
     6721 gacggtgctg ccggtgtgtt cctggcggcc aataccttcc cgaaatcgcg tgaaacgcgt
     6781 gcgccgctgg tggaagagct gtatcgcttc cgcgatcgtc tgccggaaaa actgcgttat
     6841 ctggccgatg cgccgcagca ggatccggaa ggtaataaga ctatggttcg ctttagccgt
     6901 aaaaccaaac atcaatatgt ctcttcggaa aaagacggaa aggcgactgg ctggtcagca
     6961 ttttatgttg atggcaaatg ggttgaaggg aaaaaataac ctttaattct gtcaggtttt
     7021 tataaacaaa gggtcgcgaa agcggccctt tt
//
LOCUS       DAZKFU020000238         7008 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZKFU020000238 DAZKFU020000000
VERSION     DAZKFU020000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.240"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(38..3940)
                     /gene="hrpA"
                     /locus_tag="ACHOQQ_RS20015"
                     /old_locus_tag="pgaptmp_004003"
     CDS             complement(38..3940)
                     /gene="hrpA"
                     /locus_tag="ACHOQQ_RS20015"
                     /old_locus_tag="pgaptmp_004003"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGU4472198.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4141..4746
                     /gene="azoR"
                     /locus_tag="ACHOQQ_RS20020"
                     /old_locus_tag="pgaptmp_004004"
     CDS             4141..4746
                     /gene="azoR"
                     /locus_tag="ACHOQQ_RS20020"
                     /old_locus_tag="pgaptmp_004004"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGU4472199.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4800..6092)
                     /gene="ynbD"
                     /locus_tag="ACHOQQ_RS20025"
                     /old_locus_tag="pgaptmp_004005"
     CDS             complement(4800..6092)
                     /gene="ynbD"
                     /locus_tag="ACHOQQ_RS20025"
                     /old_locus_tag="pgaptmp_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGU4472200.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATIMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIKIALPYVVG
                     ACSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCLRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVNEAISYIRARRPQIVLTDEHKAMLRLWENR"
     gene            complement(6106..>7008)
                     /locus_tag="ACHOQQ_RS20030"
                     /old_locus_tag="pgaptmp_004006"
     CDS             complement(6106..>7008)
                     /locus_tag="ACHOQQ_RS20030"
                     /old_locus_tag="pgaptmp_004006"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HGU4472201.1"
                     /translation="KSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAM
                     KLFGTHSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQR
                     KTHLQILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNV
                     AQGQEMIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSL
                     AGLANAIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAG
                     FDKCTQRIDEWGIFTVSMAVRRDN"
BASE COUNT         1686 a         1957 c         1697 g         1668 t
ORIGIN      
        1 cttaccgggt tttatattta tcaggcaaat agcagcgtta accgctaatc tgctccatcg
       61 cctgcaaaat acgcttatct gaaatcggat aaggcgtacc aagttgttga gcgaagtaac
      121 taacgcgcag ctcttctatc atccaacgga tctctttcac gtcttcatcc tcacgacgtg
      181 caggcggcag tttgttgatc cattgctgcc acgcctgctg gacgttttcg actttcagca
      241 tctgtgcacg atcgcgatgt ggatcaaccg ccagcttttc cagccgtttt tcaatcgcct
      301 gcaaataacg cagcgtgtcg cccagccgtt tgaagccgtt accagtgaca aaaccgcgat
      361 ataccaaccc gcccatctgc gctttaatgt cagaaagccc cagcgccatg gtcatatcca
      421 cccgcccttt cagacgcttg ttgatattga acactgccgt aaggatttgc tcgacctgct
      481 tcgcaatatc caccaccgtg tcgttcagtt cggcacgcac tttttcatgc agcgcagcaa
      541 agccttcttc cgtccagacc gggccaccat tggcgtcgat caatttatcc acaccgcagg
      601 agatacagtc gtcgatcagc tccagcactt tgccatacgg gttaaagtac agtccaagct
      661 tggctttgtt tggtaacttc tcatgtaaat acttgatcgg cgatggaata ttcagcagca
      721 gtagacggcg aagaccgttc cacattgcct gtttttgctc cagcgggtta tcaaacagtt
      781 tgatcgccac actgtcgcgc tcatccacca gcgccggcca cgccttcact ttgtagttgc
      841 cgcgcttctg ctcgtagctt tccggcagtt gcccaaaact ccagatatgt aagccgctct
      901 gctcgatacc gtcatccgcc accgcagaca gcgtttcctg cactttgcct ttcagcgcat
      961 ctttcagatc ttgtagcgag cgcccttctt ttagcttctt gtttttgtca tccaccacgc
     1021 ggaaggtgat tttcaggtga tcgggcacct gatcccagtg ccagtcttcg cggtcaacgg
     1081 taacgccggt catccgccgt aactcgcgct caaggctgtc aagcaacggt aactccagcg
     1141 gtgtgacgcg gcctaaaaac gcttcggcgt agtttggcgc gggtacaaaa ttacggcgta
     1201 ccggtttcgg caacgattta atcagagcaa tcaccagttc gcggcgcaga ccggggatct
     1261 gccattcaaa cccgctttcc tcaacctggt taagtaacgg cagcggaata tgtacggtca
     1321 caccgtcagc atccgcgccg ggctcaaact gatagctcaa acgcagcttg agattgccct
     1381 gatgccagaa gttcggataa tccagcttgc tgatcttttc cgccccttct ttgatcaaca
     1441 tgctcttttc aaagttgagc aaatcaggcg tttcgcggct gacttttttc caccaactgt
     1501 cgaagtgacg agcggagatt acatcgtggc tgatgcgctg gtcgtagaac tcaaacaacg
     1561 tttcgtcatc aaccagaata tcgcggcgac gtgatttgtg ttccagctct tccacttcgg
     1621 cccgcagttt caggttttca cggaagaatg cgtgacgcgt ctgccagtca ccttccacca
     1681 gcgcgtggcg aataaagagt tcacgacata acgccggatc gatctggctg tagttgacct
     1741 tgcgcgcggc aacaatcggc aaaccataaa cagtgacttt ttccgttgcc atcaccgcgc
     1801 cctgcgcccg ttcccagtgc ggttcgctgt aggtacgttt aatcaaatgc tgagcgacag
     1861 gctccaccca ttccgggtcg atacgcgcag caatgcgccc ccacaggcgg ctggtttcta
     1921 ccagttccgc caccattacc catttcggcg gttttttgaa taaaccagaa ccggggaaga
     1981 tggagaaacg cgcgttacgt gcgccggtat attcttgttt atcggcatct ttcatgccga
     2041 tatgggaaag caaacccgtc agcaacgcaa tgtgaatttc acgatactcc gccggttcgc
     2101 tgttcaccgg aatgccgagt tctttcacca cctggcgcaa ctgggtgtag atatcctgcc
     2161 attcgcgcac acgcagatag ttgagataat cggtacgaca caggcgacgg aaggcgttgg
     2221 aagaaagcgc cttttgctgc tcgccaagat aattccacag attcacaaac gcgaggaagt
     2281 cagactcttt gtcgtggaag cgacgatgtt tttcgtccga tgcctgctgt ttgtccatcg
     2341 gacgttcgcg cggatcctga atggagagcg cagacgtgat aatcatcgcc tcacgcacgc
     2401 agccatgttt ttgcgcttcc agcaccatac gcgccagacg tgggtcgaca ggcaactgcg
     2461 agagctggcg accgagcggc gtcagtttat aggcgctggc ctgttcatcc gtggtgatcg
     2521 cgcccagttc ttcgagcaga cgcacgccat cctggatatt gcgtttatcc ggtgcttcga
     2581 caaacgggaa cgcagcgata tcgcccagcc ccagcgcggt catctgcaaa ataaccgagg
     2641 ccaggttggt acgcagaatc tccggatcgg taaactccgg gcgcgagagg aaatcgtctt
     2701 cggaatagag acgaatacag atcccttcgg acacacgacc acagcggcct ttacgctgat
     2761 tggcagacgc ctgcgaaatg ggctcaatcg gcaaacgctg cactttggtg cgatagctgt
     2821 agcggctgat acgcgctgta ccggggtcga taacgtattt aatccccggt acggtcagcg
     2881 acgtttccgc gacgttggtc gccagcacaa tgcgccgtcc gctatgcgac tggaacaccc
     2941 tattctgttc gctattcgaa agccgcgcat aaagcggcaa gatttccgta tgtcgtaagt
     3001 tcagtttgtt cagtgcatca gcggtatcgc gaatttcccg ctcgccgctc ataaagatca
     3061 gaatgtcgcc ggggctttcc tggctcagtt cgtctacggc gtcaaaaatc gcctgcaatt
     3121 gatcgcgctc ggtgtcatcg gcttcttcaa caatcgggcg atagcgcact tccaccggat
     3181 aggtccgacc ggagacttcg ataatcggcg cattattaaa gtggcgcgaa aagcgttccg
     3241 ggtcgatagt cgcggaagtg ataatgattt ttaggtcagg acgccgcggt agcaactctt
     3301 tcaaatagcc gagcaaaaaa tcgatattca ggctgcgttc gtgcgcttcg tcaataatga
     3361 tagtgtcgta ctgcatcagc aggcggtctt gctggatctc cgccagcagg ataccgtcgg
     3421 tcatcagctt gaccatcgtg ttatcactta cgtgatcgct gaaacgcact ttgtaaccga
     3481 tgcaaccgcc cggctccgtt ttcagctctt ccgcaatacg gtttgccact gttcttgccg
     3541 ccagacgacg cggctgggta tgaccgatca gccctttaat accgcgcccc agctccatac
     3601 agattttcgg tagctgagtc gttttaccgg aaccggtttc cccggcgacg atcaccacct
     3661 ggtgatcacg aatcgcttcg agaatgtcct gctttttctg actaactggt aaattgtccg
     3721 ggtaagtaat ttccggtcgt gccgcttcac gcagcaggac tttccctgcc gcctggtcaa
     3781 tctctttcgc catctcctgg aaaatggcct gttgtgcatc aggattttta accttcttca
     3841 caccatgcag acggcgagaa aaacgcagtc tgtcacgcag catcagcgaa tccagccgct
     3901 gctgcaaggc tgtaaaggtc aatttttgtt gttctgtcat aacgttagag ggcagtaggc
     3961 tgcctgatta attttctttt aaatgataga tatagagtac cacatcgcgg ctttctatgc
     4021 gttgttcaat aaattcgaac ataggcttcg atttattgcg ctatctctgt ggcaggattg
     4081 tgaataaagt gtcagcaagc aacggggcat cgcccattca aacatctata aggaaacacc
     4141 atgagcaagg tattagttct taaatccagc atcctggcag ggtactctca gtctaatcag
     4201 ttgtccgatt attttgttga acaatggcgc gaaaagcact ccgctgatga aatcaccgtt
     4261 cgcgacctgg ctgcaaatcc gattccggta ctggatggcg aactggttgg tgcgctgcgt
     4321 ccgagcgatg cgccgctgac tccgcgtcag caggaagctc tggcactttc cgatgagctg
     4381 attgccgagc tgaaagccca tgacgttatc gttattgcgg caccgatgta taacttcaac
     4441 atctcgactc agctgaaaaa ctattttgac ctggttgcac gtgcaggcgt tactttccgc
     4501 tataccgaga atggtccgga aggtctggta acgggtaaaa aagccatcgt tattaccagc
     4561 cgcggtggga tccacaaaga tggaccaacg gacctggtga cgccgtatct gtccacgttc
     4621 ctgggcttta tcggcattac tgatgtgaaa tttgtcttcg ccgaagggat cgcatacggt
     4681 ccggaaatgg cagcgaaagc gcagtctgac gcgaaagcag ccatcgacag cattgttgct
     4741 gcataaccgg acacggtctt gatgtggact gtagatattc agtccacatc tcaatccact
     4801 tacctgtttt cccacaatct cagcattgct ttgtgttcgt ctgtcagcac aatctgcggg
     4861 cgtctggctc gaatatagct aatcgcttca ttaacggttt tacagtgtcc gtaacataac
     4921 aaccatgccg ccaccaccag cgcactgcgc gataatccca atgcacaatg gaccagaacg
     4981 ctgccttgct cttcgcgtaa tgtttccagc atcgccacgg cctgtcggag ctccccctct
     5041 tccggaacca ccagatccag catcggtaca caaaaataga gtcgatcttt tgtggctcgt
     5101 ccgcgaggga attcaaaggt gacgtccaga accgcattct gtgccggaat atgtcgtgga
     5161 aacgccccta aataaacacc agcagtcatt ttgctcaccg gctccaggcg aagacaaaac
     5221 caacgcatag acagccacat cccgatgcgc cagggcaatg tcagccagta aacggcgggc
     5281 gggagtttcc cctgactatc tttccctgtt gttatcgcgc caagcccacc gtacccacgg
     5341 ccaatgatga atagcgataa tactggccaa cataaccaga ctgaccacca taactgaagc
     5401 attatcatta gctccatcaa cacaatgcac gagcacgcgc ctacgacata tggcagtgct
     5461 attttgattc gacgttgatc aggtttctga taattccaac gacggtcgac gggcaccatc
     5521 cagtcaatta acatacctac cgccagccct gtgatgacat caataaaatg atgctgccag
     5581 gttgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cgcagacttt acgccacctc
     5641 acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat atgcagcgaa
     5701 ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca tcctgtcacc
     5761 ccactcactt caggacggat aaaactaaac ttcagcggat agagaaaaaa accgcagcag
     5821 gccattatcg ttgccagaat aagccggtga accagtcggc gctgttcgaa tgtcgaacta
     5881 caaacgaaca acgaaaaccc atataaaaga tccagactcc agtaaggaac aatagtccag
     5941 gggagaaaag ggatcgccgt ttcccagccg aatacctgac tggggatatc atggctgtta
     6001 aagtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa aaacggggcc
     6061 agcaacaata accagccagc gccttgtagc aatacgttcc ggcgttcagt tatcacgacg
     6121 caccgccatc gaaaccgtaa aaatgcccca ctcatcaatc cgttgagtgc atttatcaaa
     6181 tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca ttacccacgg
     6241 tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg ggtgccacgg
     6301 ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggcaagac ctgcgagtga
     6361 gttttttacc tgctcatttc cgggaaaaag ctcatacagt ccagaaacaa tcgccagcgt
     6421 aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct gttcaaaacg
     6481 cacccgccca gacattcccc gttgggcaat catctcttgc ccctgagcaa catttaactc
     6541 actgtaatca cgtaacaaaa tatcgcttac ggcaggttcg tttgccagcg catccagtac
     6601 atagcgccca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt tggcgtggag
     6661 atcggcaacg gcttgtttaa tcagtatttg taaatgggtt ttgcgctggc gaataccgcg
     6721 ccagccaaca ctgttcaggt agattttgtc gattaagcgc ccgaatgcgt tactaccttg
     6781 cggttgattt tgatagacat aatccagcga actgcctgaa tcaaagccgg tgctcattcc
     6841 gagatgcagg cccgcagagt gcgtcccgaa caatttcatc gctttacgca taaagcgata
     6901 cgccaaatca accggcgata atggcacggg tccacctgaa aggagccgcc agcgatccgc
     6961 tgatggtcca gtgcggtctt catgctgata atcaaatttt tgtgactt
//
LOCUS       DAZKFU020000239         6930 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZKFU020000239 DAZKFU020000000
VERSION     DAZKFU020000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.241"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..548
                     /gene="ftnA"
                     /locus_tag="ACHOQQ_RS20035"
                     /old_locus_tag="pgaptmp_004007"
     CDS             51..548
                     /gene="ftnA"
                     /locus_tag="ACHOQQ_RS20035"
                     /old_locus_tag="pgaptmp_004007"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGU4472202.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(586..825)
                     /locus_tag="ACHOQQ_RS20040"
                     /old_locus_tag="pgaptmp_004008"
     CDS             complement(586..825)
                     /locus_tag="ACHOQQ_RS20040"
                     /old_locus_tag="pgaptmp_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGU4472203.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1016..2227
                     /gene="tyrP"
                     /locus_tag="ACHOQQ_RS20045"
                     /old_locus_tag="pgaptmp_004009"
     CDS             1016..2227
                     /gene="tyrP"
                     /locus_tag="ACHOQQ_RS20045"
                     /old_locus_tag="pgaptmp_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGU4472204.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2289..2954)
                     /gene="yecA"
                     /locus_tag="ACHOQQ_RS20050"
                     /old_locus_tag="pgaptmp_004010"
     CDS             complement(2289..2954)
                     /gene="yecA"
                     /locus_tag="ACHOQQ_RS20050"
                     /old_locus_tag="pgaptmp_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGU4472205.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAYPQEQAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3150..3236)
                     /locus_tag="ACHOQQ_RS20055"
                     /old_locus_tag="pgaptmp_004011"
     tRNA            complement(3150..3236)
                     /locus_tag="ACHOQQ_RS20055"
                     /old_locus_tag="pgaptmp_004011"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3200..3202),aa:Leu,seq:taa)
     gene            complement(3249..3322)
                     /locus_tag="ACHOQQ_RS20060"
                     /old_locus_tag="pgaptmp_004012"
     tRNA            complement(3249..3322)
                     /locus_tag="ACHOQQ_RS20060"
                     /old_locus_tag="pgaptmp_004012"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3288..3290),aa:Cys,seq:gca)
     gene            complement(3377..3452)
                     /locus_tag="ACHOQQ_RS20065"
                     /old_locus_tag="pgaptmp_004013"
     tRNA            complement(3377..3452)
                     /locus_tag="ACHOQQ_RS20065"
                     /old_locus_tag="pgaptmp_004013"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3417..3419),aa:Gly,seq:gcc)
     gene            complement(3604..4152)
                     /gene="pgsA"
                     /locus_tag="ACHOQQ_RS20070"
                     /old_locus_tag="pgaptmp_004014"
     CDS             complement(3604..4152)
                     /gene="pgsA"
                     /locus_tag="ACHOQQ_RS20070"
                     /old_locus_tag="pgaptmp_004014"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGU4472206.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4209..6041)
                     /gene="uvrC"
                     /locus_tag="ACHOQQ_RS20075"
                     /old_locus_tag="pgaptmp_004015"
     CDS             complement(4209..6041)
                     /gene="uvrC"
                     /locus_tag="ACHOQQ_RS20075"
                     /old_locus_tag="pgaptmp_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGU4472207.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6038..6694)
                     /gene="uvrY"
                     /locus_tag="ACHOQQ_RS20080"
                     /old_locus_tag="pgaptmp_004016"
     CDS             complement(6038..6694)
                     /gene="uvrY"
                     /locus_tag="ACHOQQ_RS20080"
                     /old_locus_tag="pgaptmp_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGU4472208.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1676 a         1792 c         1650 g         1812 t
ORIGIN      
        1 aaattatagt gacgccacag caacaaatat aacctttgtg gagcactatc atgctgaaac
       61 cagaaatgat tgaaaaactt aatgagcaga tgaacctgga actgtactct tcactgcttt
      121 atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc gcgttcctgc
      181 gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac ctgactgata
      241 ccggcaattt accgcgtatt aataccgttg aatctccgtt tgctgaatat tcctcacttg
      301 atgaattatt ccaggaaacc tataaacacg aacaattaat cacgcagaaa attaacgaac
      361 tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg caatggtatg
      421 tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa ttaagcctgg
      481 caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga actctctacc ctcgacacac
      541 aaaactaatg ctctccggcg gcaggttttc tgccgccgtt tcaaatcagt gacggcaaat
      601 cttactctga tcggtcgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag
      661 aaacgctacc agctctcccg ctgtcatccc ttctgccgag caagtgtgaa aacgtgcctg
      721 ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca tgcgtatatt gctcgcctga
      781 ttcaatcatc atatttaaca cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa
      841 aaaagcctag cgtagcgatt accgcttatg aagactttgc gccagcgcag gactgaatgc
      901 ttttattgta catttaaatt tacaccatat gtaacgtcgg tttgacgaag cagccgttat
      961 gccttaacct gcgccgcaga tatcactcat aaagatcgtc aggacagaag aaagcgtgaa
     1021 aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc acaattggcg caggcatgct
     1081 ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt actttaatct tgttgattgg
     1141 gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc agcatgttcc
     1201 ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg gtcaatggct
     1261 gacgggcttc agtatgatgt tcttaatgta tgctctgact gcggcataca tcagcggtgc
     1321 cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt cggcaaccgc
     1381 tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt tgtgttggaa catcgctggt
     1441 cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg tgatgctggt
     1501 attgctgcta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc aacaggggct
     1561 ggcactgtct gcaatcccgg tgatttttac gtcgtttggt tttcacggta gcgtgccgag
     1621 tattgtcagc tatatggatg gcaacattcg taagctacgc tgggtgttta taaccggtag
     1681 tgcgatcccc ctggtggcat atattttctg gcaggtggcg acgcttggca gcattgattc
     1741 aacaaccttt atgggattgc tggctaatca tgccggatta aacgggctgt tacaggcgtt
     1801 acgcgaaatg gtggcctctc cgcatgttga gctggcagtg catttatttg ctgatttagc
     1861 cctcgccacg tcatttctcg gcgttgcgtt aggcttattt gattatctgg ctgatttgtt
     1921 tcagcgttca aataccgtgg gtggaagatt gcaaactggt gcaattacct ttctgccgcc
     1981 gttggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt acgccggtgt
     2041 ggcgctggcg gtactggcat tgattatccc ttcgctgttg acctggcaaa gcagaaagca
     2101 caatcctcaa gcgggttacc gggtcaaagg tggtcgtccg gcgctggtgg tggtgtttct
     2161 ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcagggttgt taccagaagt
     2221 ggggtgatta gatagcctca aattccttat tgggtgccag aattaacgct gacacccaat
     2281 ttgacttctt aatgcaggca gcactgctta aatttcttac cactaccgca cgggcaagga
     2341 tcgttacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttg ttcctgcgga
     2401 tacgccatcc agtatgcatg taaatcaagc gccgccagtc gaatggcatc tacgctctct
     2461 tcaaacgctt ctggcgacat cttctctacc cgctcgaagt tttcctcagt accgtgcagc
     2521 gctatcgcct ccagcgctgg ttttaacgaa tcgggcaatg ttgaccagtc agaaagtgcc
     2581 acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct gccatcaact
     2641 tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg ctctgcggta
     2701 tcggccatat gttgaaaagc caggttcata aagcgcgtca tctctttctc tgacgcccag
     2761 cgcggcacat agtcagcccc accccacacg gcaaccagcc actgtgccgg ttcaatctct
     2821 tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc aaggatggcg
     2881 tggtcagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact ttcgtttaac
     2941 ggtcccgttt tcatacgctt ttccttgtgg atctcaactc gccagcacct atattacatg
     3001 ccggtccgta tcagagatac tttttgagtg gctttgccgg tgattaaaaa tcaaggaggg
     3061 tgtaacgaca agttgcaggc acaaaaaaac cacccggagg tggtttcacg acactgctta
     3121 ttgctttgat tttattctta tctttcccat ggtacccgga gcgggacttg aacccgcaca
     3181 gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat ccgggctcgg
     3241 gaagaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc aatccgctac
     3301 ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat ccgcttgagg
     3361 ccgatgtctt ttaaactgga gcgggaaacg agactcgaac tcgcgacccc gaccttggca
     3421 aggtcgtgct ctaccaactg agctattccc gcattcatca agcaatcagt taatcacttg
     3481 attttattat cgtctggcaa tcagtgccgc cgttcgatgc gttgcattct acttacctgg
     3541 cgcgatgagt caacgatatt tttcaccact tttgatcgtt tgctgaaaat tgcgccgaaa
     3601 cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa tattgcaaca ttgaccacag
     3661 agtcagtacc gcagccacaa agaaaagtgc aatgccggcg tactcaaccc aaatgttcgg
     3721 acgccacagc agccaggcca acgccaccat ctgggcagtg gttttcactt tcccaatcca
     3781 ggagacagcc acgctactgc gtttacccaa ctccgccatc cattcgcgta gcgcagaaat
     3841 aataatttca cgggcgatca tcgttgccgc cggtaaggtc acccaccagc tgtggtaatg
     3901 ctcggttacc agcaccatgg cgatagccac gagaacttta tctgctacag ggtcaaggaa
     3961 agcaccaaac cgggtactct ggttccagcg gcgtgccaga aaaccatcga accagtcagt
     4021 caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc gaccaggtga caggcagata
     4081 aaagaccaat acaaagaatg ggataaggat gacacggaac agtgtaagca acgtagggat
     4141 attaaattgc ataatgacgg gtaactatct gttgtcagta agattacccc tatgttgcta
     4201 cagagacatc aatgtttcaa cgaccagaag atcttttctg ccagaccttg cgaaataccc
     4261 ggcacttttg caatttcctc gacgctggcg ttacgtaaac cttgcaaacc gcccatatat
     4321 ttcaacaaca tttgccggcg ttttggcccg acgccttcaa tggtttccag ggaactggta
     4381 tttttgacct tcgcccgttt tttacggtgc ccgccaatcg cgtgatcatg tgattcatcg
     4441 cgaatatgct ggataacatg cagcgcggga gaatctggcg gcaaactgaa tccctcacct
     4501 tccggctcaa agaacagcgt ttccagccca gccttacgat ctgctccttt ggcaacgcca
     4561 agtagcagcg gatgattttt atcccatgag acatccagtt cggcgaaaac atttttcgcc
     4621 tgcgcaagct ggcctttgcc gccgtcgata agtatcacat ccgggatctt actgtcgtcg
     4681 atggctttac cataacgccg acgcagcacc tgattcatcg ccgcataatc atcgcccggc
     4741 gtgatgccag taatgttata gcgccgatac tccgcacgca gcgggccgtt agcatcaaac
     4801 accacacagg aagcgacggt ttgttcaccc atggtatggc tgatgtcaaa gcactccatc
     4861 cgcttcactt ccggcaattt caacacgctg gcaagtgctg taagccgctg gtgaacggta
     4921 gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat tggtgcgcgc gagtttcagg
     4981 taacgcgccc tatcgccacg aggtttggtt tgaacattaa tcttgcgtcc cgccagttct
     5041 gaaagggaat cggcgagcag cgttttatcg ctaagattaa aatcgagcag gatctcaccc
     5101 ggtaaggtgc gcatctggct gccttgtaaa tagaactgtc ctacgaaggt ttccaccacc
     5161 tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc tgcggctgcc aagcactttg
     5221 ccctgacgaa tgaacaatac gtggacacaa gccatgcccg catcgaacgc cacaccaata
     5281 acgtcaaggt cgtcgccggt attggaaaca aactgttttt cggtgacgcg tcgcaccgcc
     5341 tgaatttggt cgcgaatacg tgccgcttct tcaaactcca gattctggct ggcagtttcc
     5401 atacgactaa tgagttgcgt aagcacctga tcatctttgc cagacaaaaa caggcgcaca
     5461 tactcgacct gctgagcgta ttcttcttca ctcaccagtc cttcaacgca cggtcccaga
     5521 cagcgcccta tctgatattg cagacacgga cgcgagcgat tgcgataaac actattttcg
     5581 cactggcgaa tggggaaaat cttttgcagt agcgccagtg tttcacgtac ggcatagcca
     5641 ttcgggaacg ggccgaaata ttcacctttg gcatgctttg caccacgatg catcgccaga
     5701 cgcgggtggg tatcaccact caggaagata aaaggatatg atttatcatc gcgtagcaaa
     5761 acgttgtaac gcggctgata gagtttgatg tagttgtgtt ccagcaacag cgcttcggtt
     5821 tctgtatgag taaccgttac atcaatttgc tggatctggg cgaccagcgc ttcggttttg
     5881 cgcgaagcga ggttgctacg gaaatagctg gaaagccgtt ttttcaggtc tttcgctttg
     5941 ccgacataga taaccgtacc accagcatcg tacatgcgat aaacgcctgg ctggctggtt
     6001 acggttttta aaaacgcttt tgcgtcaaac tgatcactca ctgacttgat aatgtctccg
     6061 cattacacag accatggcga attgccaggt gagtcagctc aacatcgcca tgaatgttta
     6121 gtttactgaa catacgatag cggtagctgt tcaccgtttt cggactgaga ttgagctgtt
     6181 ctgagatctc attgaccttc tggcccttgg tgatcatcag cataatctgc aattcacgtt
     6241 cagacaaact ggcaaatggg ctttctgttt tttctggttc gatctggctt aacgccattt
     6301 gttgagcgat gtcagaagca atgtaacgct gccctgaata gacagaacgg atcgcactca
     6361 cgacttcctg cggagccgcg cctttgctga ggtagcccgc agcaccggcc tgcatgactt
     6421 tcgctggtaa agggttttct gtatggacgg taagcatgat gattttgaca tcagctgtgg
     6481 aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc cggcatactc atgtccatta
     6541 gcaccacgtc aacggcattt gcccggcacc acttaacggc gtcttcaccg cacgatgcct
     6601 caccgacgac ttttataccc tttatatctt ccagaatgcg tcgtatccct gcgcgcacca
     6661 gttcgtggtc atcaacaagt agaacgttga tcaaaggaat atctccagaa atagggataa
     6721 cgctactgat agttagtcat tcgtatatta gcggttttta ttgcaacttt gaaacgttaa
     6781 aaatggtggg tattcgattt ttctctcgtt tttgtaaatt cgattgtcca cagttatggt
     6841 cacgcccgtc accaacggct tcagggatcg tttcacagta agccctgcga tgaaatgtga
     6901 caaaaattga ctttattcag caaaaatgaa
//
LOCUS       DAZKFU020000240         6905 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZKFU020000240 DAZKFU020000000
VERSION     DAZKFU020000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.242"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            424..1413
                     /gene="araF"
                     /locus_tag="ACHOQQ_RS20085"
                     /old_locus_tag="pgaptmp_004017"
     CDS             424..1413
                     /gene="araF"
                     /locus_tag="ACHOQQ_RS20085"
                     /old_locus_tag="pgaptmp_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGU4472209.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1483..2997
                     /gene="araG"
                     /locus_tag="ACHOQQ_RS20090"
                     /old_locus_tag="pgaptmp_004018"
     CDS             1483..2997
                     /gene="araG"
                     /locus_tag="ACHOQQ_RS20090"
                     /old_locus_tag="pgaptmp_004018"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGU4472210.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3012..3998
                     /gene="araH"
                     /locus_tag="ACHOQQ_RS20095"
                     /old_locus_tag="pgaptmp_004019"
     CDS             3012..3998
                     /gene="araH"
                     /locus_tag="ACHOQQ_RS20095"
                     /old_locus_tag="pgaptmp_004019"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGU4472211.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4165..4965
                     /gene="otsB"
                     /locus_tag="ACHOQQ_RS20100"
                     /old_locus_tag="pgaptmp_004020"
     CDS             4165..4965
                     /gene="otsB"
                     /locus_tag="ACHOQQ_RS20100"
                     /old_locus_tag="pgaptmp_004020"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGU4472212.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4940..6364
                     /gene="otsA"
                     /locus_tag="ACHOQQ_RS20105"
                     /old_locus_tag="pgaptmp_004021"
     CDS             4940..6364
                     /gene="otsA"
                     /locus_tag="ACHOQQ_RS20105"
                     /old_locus_tag="pgaptmp_004021"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGU4472213.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6371..6799)
                     /gene="uspC"
                     /locus_tag="ACHOQQ_RS20110"
                     /old_locus_tag="pgaptmp_004022"
     CDS             complement(6371..6799)
                     /gene="uspC"
                     /locus_tag="ACHOQQ_RS20110"
                     /old_locus_tag="pgaptmp_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGU4472214.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGN"
BASE COUNT         1734 a         1620 c         1806 g         1745 t
ORIGIN      
        1 aattaatggc gaaatgaatg acgcgcattt tccacatctt tgagttgtgg ttattacacc
       61 atttcaaaaa ctcaacgcca ggtaatgcgg cctattgact ggttaaaaag aagacatccc
      121 gcatgggtac caaagacaac aaggatttcc aggctaatct tatggattaa tctgctgtgc
      181 attcaacaat ttgtctgaca aattggcttt tccttatgtc ttttcccgct aaatttatgc
      241 acgttctcac tgtaattctg cgatgtgata ttgctctcct atggagaatt aatttctcgc
      301 taaaactatg tcaacacagt cacttatctt ttagttaaaa ggtaatgctt tgttttccga
      361 ttaatttaac gaatgtcatt cgtttttgcc ctacacaaaa cgacactaaa gctggagaga
      421 accatgcaca aatttactaa agccctggca gccattggtc tggcagccgt tatgtcacaa
      481 tccgctatgg cggagaacct gaagctcggt ttcctggtga agcaaccgga agagccgtgg
      541 ttccagaccg aatggaagtt tgccgataaa gccgggaagg atttagggtt tgaggttatt
      601 aagattgccg tgccggatgg cgaaaaaaca ttgaacgcga tcgatagcct ggccgccagt
      661 ggcgcaaaag gtttcgttat ttgtactccg gacccgaaac tcggctctgc catcgtggcg
      721 aaggcgcgtg gctacgatat gaaagtcatt gccgtggatg accagtttgt taacgccaaa
      781 ggtaagccaa tggataccgt tcctctggtg atgatggcgg cgaccaaaat tggcgaacgt
      841 cagggccagg aactgtataa agagatgcag aaacgtggct gggatgtcaa agaaagcgcg
      901 gtgatggcga ttacctccaa cgaactggat acggctcgtc gccgtactac gggttctatg
      961 gatgcgctga aagctgcagg attcccggaa aaacaaattt atcaggtacc taccaaatct
     1021 aacgacatcc cgggggcatt tgacgctgcc aactcaatgc tggttcaaca tccggaagtt
     1081 aaacattggc tgatcgtcgg tatgaacgac agcaccgtgc tgggcggcgt acgcgcgacg
     1141 gaaggtcagg gctttaaagc ggccgatatc atcggtatcg gtattaacgg tgtggatgcg
     1201 gtgagcgaac tgtctaaggc acaggcaacc ggcttctacg gttccctgtt gccaagcccg
     1261 gacgtacatg gttataagtc cagcgaaatg ctttacaact gggtagcaaa aggtgttgaa
     1321 ccgaccaaat tcaccgaagt taccgacgtg gtgctgatca cgcgtgacaa ctttaaagaa
     1381 gaactggaga aaaaaggttt aggcggtaag taatttgccg gaaaaattcc cctctgcatg
     1441 atgcagaggg ggtgtgaacg accagtgatt cacggagacg ttatgcaaca gtctaccccg
     1501 tatctctcat ttcgcggcat cggtaaaacg tttcccggcg ttaaggcgct gacggatatt
     1561 agttttgact gctatgccgg tcaggttcat gcgttgatgg gtgaaaatgg cgcaggaaaa
     1621 tcaactctct taaaaatcct cagcggcaac tatgcgccaa ccacgggttc tgtagtgatt
     1681 aatgggcagg aaatgtcctt ttccgacacg accgcagcac ttaacgcggg tgtggcgatt
     1741 atttaccagg aactgcatct cgtgccggaa atgaccgtcg cggaaaacat ctatctcggc
     1801 cagctgccgc ataaaggcgg cattgtgaat cgctcattgc tgaattatga ggcgggttta
     1861 caacttaaac atcttggtat ggatattgac ccggacacgc cgctgaaata tctctccatt
     1921 ggtcagtggc agatggttga aatcgccaag gcgctggcgc gtaacgctaa aattatcgcc
     1981 tttgatgagc caaccagctc cctttctgcc cgcgaaatcg acaatctttt ccgcgttatt
     2041 cgtgaactgc gaaaagaggg gcgggtgatc ttatacgttt ctcaccgtat ggaagaaata
     2101 tttgccctca gcgatgccat cactgtcttt aaagatggac gttatgtcaa aacctttacc
     2161 gatatgcagc aggttgacca cgacgcgctg gtgcaggcga tggtcgggcg cgacattggc
     2221 gatatctacg gctggcaacc gcgtagttat ggcgaggagc gcctgcgtct tgatgctgtg
     2281 aaagcaccag gcgtgcgtac gccaataagt ctggcggttc gcagtggtga aattgttggg
     2341 ctgtttggtc tggtaggggc ggggcgtagc gaattaatga aaggcatgtt tggcgggacg
     2401 caaatcaccg ccggtcaggt ttatatcgac caacagccga tcgatattcg taaaccgagc
     2461 cacgccattg ccgcaggcat gatgctctgc ccggaagatc gcaaagcgga aggcattatt
     2521 cctgtgcact ccgttcgcga caatatcaac atcagtgcca gacgtaaaca tgtgctcggc
     2581 ggttgtgtaa tcaacaacgg ttgggaagaa aacaatgccg atcaccacat tcgttcgctc
     2641 aacatcaaaa cgccgggcgc ggagcaactg atcatgaatc tctcaggcgg aaatcagcaa
     2701 aaagccattc tgggccgctg gttatcggaa gagatgaagg tcattttgct ggatgaacct
     2761 acgcgcggca ttgatgttgg cgctaagcac gaaatatata acgtaattta tgcgctggcg
     2821 gcgcagggcg tggcggtgct gtttgcctcc agcgacttac ctgaagtcct cggcgttgcc
     2881 gaccggattg tggtgatgcg ggaaggtgaa atcgccggtg aattgttaca cgagcaggca
     2941 gatgagcgtc aggcactgag ccttgcgatg cctaaagtca gccaggcagt tgcctgagta
     3001 aggagagtat gatgtcttct gtttctacat cgggttctgg cgcacctaag tcgtcattca
     3061 gcttcgggcg tatctgggat cagtacggca tgctggtggt gtttgcggtg ctttttatcg
     3121 cctgtgccat ttttgtcccg aactttgcca ccttcattaa tatgaaaggg ttgggcctgg
     3181 caatttccat gtcggggatg gtggcgtgtg gcatgttgtt ctgccttgct tccggtgact
     3241 ttgacctttc tgtcgcctcc gtaattgcct gtgcgggtgt caccacggcg gtggttatca
     3301 acctgactga aagcctgtgg attggcgtgg cagcggggtt gctgcttggc attctctgtg
     3361 gcctggtcaa tggctttgtt atcgccaaac tgaaaataaa tgctctgatc acgacactgg
     3421 caacgatgca gattgttcga ggtctggcgt acatcatttc cgacggtaaa gcggtcggta
     3481 tcgaagatga aagcttcttt gcccttggtt acgctaactg gttcggtctg cctgcgccaa
     3541 tctggctcac cgtcgcgtgt ctgattatct ttggtttgct gctgaataaa accacctttg
     3601 gtcgtaatac cctggcgatt ggcgggaacg aagaggctgc gcgtctggcg ggtgtaccgg
     3661 ttgttcgcac caaaattatt atctttgttc tctctggcct ggtatctgcg atagccggaa
     3721 ttattctggc ttcacgtatg actagtgggc agccaatgac gtcgattggt tatgagctta
     3781 ttgttatctc cgcctgcgtt ttaggtggcg tttctctgaa aggtggcatc ggaaaaatct
     3841 catatgtggt ggcgggtatc ttaattttag gcaccgtgga aaacgccatg aacctgctta
     3901 atatttctcc tttcgcgcag tacgtggttc gcggcttaat cctgctggca gcggtgatct
     3961 tcgaccgtta caagcaaaaa gcgaaacgca ctgtctgatg cttttttcca caacaatcta
     4021 acgttttttc ccaccacagc cagccgccac aacggttggc tgttcttcgt tgcaaatggc
     4081 gacccccgtc acactgtcta tacttacatg tctgtaaagc gcgttctgcg caaaacaata
     4141 agaaaagaga aggaggagaa ccgggtgaca gaaccgttaa ccgaaacccc tgaactatcc
     4201 gcgaaatatg cctggttttt tgatctcgat ggaacgctgg cggaaatcaa accgcatccc
     4261 gatcaggtcg tcgtacctga caatattctg caaggacttc agctactggc aaccgcaagt
     4321 gatggtgcat tggcattgat atcagggcgc tcaatggtgg agctggacgc actggcaaaa
     4381 ccttatcgct tcccgttagc gggcgtgcat ggggcggagc gccgtgacat caatggtaaa
     4441 acgcatatcg ttcatctgcc ggatgcgatt gcgcgtgata ttagcgtgca actgcatacg
     4501 gtcatcgccc agtatcccgg cgcggagctg gagacgaaag ggatggcttt tgcgctgcat
     4561 tatcgtcagg ctccgcagca tgaagacgca ttaatgacat tagcgcaacg tattactcag
     4621 atctggccac aaatggcgtt acagcaggga aagtgtgttg tcgagatcaa accgagaggt
     4681 accagtaaag gtgaggcgat agctgccttt atgcaggaag ctccctttat cgggcgaacg
     4741 cccgtatttc tgggcgatga tttaaccgat gaatctggct tcgcagtcgt taaccgactg
     4801 ggcggaatgt cagtaaaaat tggcacaggc gcaactcagg catcatggcg actggcgggc
     4861 gtaccggatg tctggagctg gcttgaaatg ataaccaccg cattacaaca aaaaagagaa
     4921 aataacagga gtgatgacta tgagtcgttt agtcgtagta tctaaccgga ttgcaccacc
     4981 agatgagcac gccgccagtg ccggtggcct tgccgttggc atactggggg cactgaaagc
     5041 cgcaggcgga ctgtggtttg gctggagtgg tgaaacaggg aatgaggatc agccgctaaa
     5101 aaaggtgaaa aaaggtaaca ttacgtgggc ctcttttaac ctcagcgaac aggaccttga
     5161 cgaatactac aaccaattct ccaatgccgt tctctggcct gcttttcatt atcgcctcga
     5221 tctggtgcaa tttcagcgtc ctgcctggga cggctattta cgcgtaaatg cgttgctggc
     5281 agataaatta ctgccgctgt tgcaagacga tgacattatc tggatccacg attatcacct
     5341 gttgccattt gcgcatgaat tacgcaaacg gggagtgaat aatcgcattg gtttctttct
     5401 gcatattcct ttcccgacac cggaaatctt caacgcgctg ccgacatatg acaccttgct
     5461 tgaacagctt tgtgattatg atttgctggg tttccagaca gaaaacgatc gtctggcgtt
     5521 cctggattgc ctttctaacc tgacccgcgt cacgacacgt agcgcaaaaa gccatacagc
     5581 ctggggcaaa gcgtttcgaa cagaagttta cccgattggc attgagccaa aagaaatagc
     5641 caaacaggct gccgggccac tgccgccaaa actggcgcaa cttaaagcgg aactgaaaaa
     5701 cgtacaaaat atcttttctg tcgaacggct ggattattcc aaaggtttgc cagagcgttt
     5761 tctcgcctat gaagcgttgc tggaaaaata tccgcagcat catggtaaaa ttcgttatac
     5821 ccagattgca ccaacgtcgc gtggtgatgt gcaagcctat caggatattc gtcatcagct
     5881 cgaaaatgaa gctggacgaa ttaatggtaa atacgggcaa ttaggctgga cgcctcttta
     5941 ttatttgaat cagcattttg accgtaaatt actgatgaaa atattccgct actctgacgt
     6001 gggcttagtg acgccactgc gtgacgggat gaacctggtg gcaaaagagt atgttgccgc
     6061 tcaggaccca gccaatccgg gcgttcttgt tctttcgcaa tttgcgggag cggcaaacga
     6121 gttaacgtcg gcgttaattg ttaatcccta cgatcgtgac gaagttgcag ctgcgctgga
     6181 tcgtgcattg actatgtcgc tggcggaacg tatttcccgt catgcagaaa tgctggacgt
     6241 tatcgtgaaa aacgatatta accactggca ggagtgcttc atcagcgacc taaagcagat
     6301 agttccgcga agcgcggaaa gccagcagcg cgataaagtt gctacctttc ctaagcttgc
     6361 gtaggagcgg ttaattcccc gtaagtggaa ctaacagcac atcgacttca cttgaggcaa
     6421 tcactctttt cgccgagcag gatgctcgcg aaaagaaact gtgattgtga ttaccgcaaa
     6481 tcaccaaatc gaaatgatat ttgcgacaaa cttccagaat atgttcgctt aactcaccgt
     6541 aggcaataaa cgtcttgtca acgggatacc ccgcatcctg aattaactta tcaagaaagc
     6601 tttgcgtttc ttcctgcatc acgctgcgta aatcttcaag catcggcgca gctaattgat
     6661 tgtacatttc cgggtcggaa gcgagagtaa ttaaactgat gtgtcccttt actggtctgg
     6721 cgatagatac cgcttttgcc agcagttgct gactttccgg tgtaaccgca acagcgacaa
     6781 gaatattgct atagctcata acctgttcct tattctgtga acttcaggtg acattaaagc
     6841 tttactccta atggagaaac gacgcaatcc caactcagtc aaacaggtaa atattagtct
     6901 gatta
//
LOCUS       DAZKFU020000241         6900 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZKFU020000241 DAZKFU020000000
VERSION     DAZKFU020000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.243"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2740)
                     /gene="pqqL"
                     /locus_tag="ACHOQQ_RS20115"
                     /old_locus_tag="pgaptmp_004023"
     CDS             complement(<1..2740)
                     /gene="pqqL"
                     /locus_tag="ACHOQQ_RS20115"
                     /old_locus_tag="pgaptmp_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4472215.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLMALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYL"
     gene            complement(2797..5169)
                     /gene="yddB"
                     /locus_tag="ACHOQQ_RS20120"
                     /old_locus_tag="pgaptmp_004024"
     CDS             complement(2797..5169)
                     /gene="yddB"
                     /locus_tag="ACHOQQ_RS20120"
                     /old_locus_tag="pgaptmp_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU4472216.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSNVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDLFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDTWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5207..6892)
                     /gene="yddA"
                     /locus_tag="ACHOQQ_RS20125"
                     /old_locus_tag="pgaptmp_004025"
     CDS             complement(5207..6892)
                     /gene="yddA"
                     /locus_tag="ACHOQQ_RS20125"
                     /old_locus_tag="pgaptmp_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGU4472217.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVPP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1777 a         1589 c         1560 g         1974 t
ORIGIN      
        1 aaagatattg tttaacggca gtgttaacat tctctaccgt catttgtttc aacaattgct
       61 cctgctcagt ccatgctgca ggatcgtcat attgaataag actatttaca atagtgttcg
      121 ctaattgttg aacgctacgc tgttggatat cgaggctgcg ctgaacgttt tgctggtatt
      181 cgttcagttc ttgctcactg atccctttag ccagacgctt aaccatcact tcattcgcta
      241 acgttaacag ttcatcatgt cgttctggtt gacaagtaaa agccagcaaa tgactgatat
      301 ctttggcctg aggatcaacc gagaggcgag aagaaacgct gtatgcccca gatgcctgtt
      361 cacgaatatt aacgcgtaga tcttttgcca gtgcgacgtt aaaagcatcg agcgccatac
      421 gcgtcgccag attaacaggt gtccgggaat cataacgctt ccactgtgaa acctgtgcca
      481 cgggttcatt ttgttccttt acagtaaccg atgcgttgtc cgtcgcgcga gttaatggtt
      541 tacctgcggc taatggcgaa tcagagtgtt tgattgagcc taagtaacgc gtaattaacg
      601 ccatgagttt gtcttctgcg acattaccga caatgacaaa cgtgatatcc gctggagatg
      661 aaaacaactg gcgatcggca gccagcgcat ctgcggcagt aaactgtgca atctgatttt
      721 cttgcagtaa tttcgtgcgg tcatcagcat agcgcgtctc atacatctgc tgggcgaatt
      781 tctccgctgg acgctggtcg agcgttttca acgcctgaat ttgagcattt tgtagcgatg
      841 cccaaatatt gtcgttaatc gtgctgtggg tgattcgctg gttaatcaac tggaaaccag
      901 gttcagggtt attagtccgc gcgctaacgg agagcaacgt attcatgcca ctgactttac
      961 tgctcatggt taccgagttt tccgcactcc agcgtttcag gctggaagag gagagttcgc
     1021 caacgccgct tccgctaact gctttatttg ccagcgcgat aagtgatttt tgctgcgcag
     1081 ggaaacttaa atcgccttta ttcgatacgg caataatttg tagcttttgc tcttcacccg
     1141 cggattttgc cagaataacc ctggcaccat tggaaagtgt taatgatgtc agattctcag
     1201 ccagcgtttc tttgctgcta atttccgcct gtgggtcagc gtctactgtt aacgataaat
     1261 ttctgcctgg gaagatgtaa gccgccagct ttttgttggc gtactctttt tccagcgcca
     1321 ggatagccgc aggagacaat gctttttttg cggcaacctc attgtttacc atttgctccc
     1381 aaaatgcgtc ctggttcttt cttaactgct gccatttttc cgccagactt tgcacggtaa
     1441 tttgctgcca cagacgtttc gaaagttgat atgtctcttc cggcgacaag aacggcgtat
     1501 tatttaatga gctggatgcc aggcgactgg tcagcatacg taaatcacgt tcagcttgct
     1561 gatcaaccgc atttttcagc caggtgaggc gggtagattt gacatcatcg agttcttcag
     1621 cagaaaaacc atgctgatca atggttgcca actctgccat taatgcattc gcagcatcct
     1681 gcatattatc gtctcgtgca tttacacgga aaaacagcga ctgataatcg ggtgcaattt
     1741 taacgctgcg cgcagtgccg ccagaaatag tcttcaactc gcccgactgt atacgttcct
     1801 gcagacgttg attgaacagc tgaactaaca tgctccattc agcttgttcg ataaagcttt
     1861 gctcatcgtt cacttgtacc attggcaggc gataatagag tgcgatgccg ttcacccggt
     1921 tttctttatc attaatgata ttaaagcgca ggtggttttc ggcttttgtc ggccagacgc
     1981 gattttcagc tgctttgtta gccggaagct tacttaaatt atcctttatc agcgccagcg
     2041 cttctttact gtcgatatcg ccaaccacga taaaggtcat attatttggt tgataccagc
     2101 gttgataaaa ttggcgcaat tgtgccggtg tgaccgtggc gacagtatcc atcaggccga
     2161 taggttcacg gtctaaatta cgggtattag ccagcaggaa agggcggcgc gcctgagagg
     2221 tgcgccattt cgcatcctga tgggcacgcc attcctcagt aattacgccg cgttcagcgt
     2281 ctacttcgag tttttcaaag gttgcggcat tactccattc actgaagatt gccatcactt
     2341 gttgcagatt ttgtttctgc gtagttggca aactcacctg atacaccgtt tcgtcatagc
     2401 tggtataggc attaacatcg cgaccaaaac gcaggcccat tgactcaaat gtttcgatga
     2461 ctttattacc cggccatgtt tttgtgccgt taaacatcat atgttctaca aaatgagcca
     2521 cgccgcgctc attgtcttct tcctgcaatg aaccggtatg aatttgcagc cataaattta
     2581 cctgatcctt tggatgagca tgcggataaa tcatatatcg caagccattg tccaattgcc
     2641 cggtaattaa cttttcatcc tgcggtaagg cggcggcaat cagccgcccg gggagcaaca
     2701 gagttgccac taacgtcagt aagaaacaga ggtttctcat aattatctcc atgcgaaaac
     2761 cgggcgaatt tacccggtta agtaaaatcc gaactattaa aatttcatgc tgacatcaag
     2821 ccagaaagta cggccactgg cgtaggtcgc cacaccggta ttcgttgtat caaccgcggt
     2881 tttgctatcc agtacattga gaatatcggc actgatcgtc aggttttgtt gtttcagaaa
     2941 ttgtggcgtc caggacaagc gggtgtccca ggtcaggctg ctatccagtt tttcgtcaac
     3001 atactgcttg taatctgaat attcgctgat gtattgcgca ttcgtcttac ccaggataat
     3061 gcgagcttta cgcgcttctt gccaggccag cgtattcgcc cacaccaacc cgctcggttg
     3121 atgcgtgaaa tccatgttta aggagatctt taatgggtta ttaaaatctg ccactggaac
     3181 gctatcgtaa gagacaagat taccgttata aaccacctgg ttatcaccgg tattgctctc
     3241 ctcataacca ttgtttaacg acaagttgcc cttgctcttg atatagctaa agacaatttg
     3301 tgggttaata tctacctggc ggatatgcag gggttcggcc agctcaaaac tgaggttgaa
     3361 cgaatgcgtt ttggttttgc catcgttgtt atattcagta atagtggttt tagtcgcgct
     3421 gtcggtacga ctgcttttgc tgatttgatc atgcgcttca cggtaaacat agtttgcacg
     3481 cgcaataacg ttcttaccga ttttctgctg caatcccatt gccagttcat cgttataagg
     3541 cgttttcaaa tcctgataac gcgtcagggt tttattgcct gataccgatt ccgtccagct
     3601 attgcggata tcacgtaatc ccatatcaag aatattccca ccatagtaac ggttataacc
     3661 tgcggtaatc attgaggttt gatcagcaaa aatatcccat tccgtcataa agcgcgggga
     3721 gatattgtgg tttgacagat agttgtcata gtcataccgc acgccgggca ttaatgacac
     3781 attacgccag ctaatgcgat ccgccatata cagtgtgtag ttgtcaattc ccaggctgcc
     3841 tttaccttta tggtaaatgg tatggttagt tttctttccg gcagcattaa tcacatagga
     3901 ttcagactgg ttatggcgtt cagtccatgc atcggaatag atgtattccg cgccgaagta
     3961 gggttgatgc gaaacattac ccacggcgaa tttttgccag tccaggcgtg ttttgaaggt
     4021 gtaattatct acagcctggg aaatgtgtcc taatccgcca cgggtgcaac gccctgtaat
     4081 atcaccatat gtacatgaaa gttcggtgta ccaggtgtca tgatcgtgac gggtataatc
     4141 actaatatga tcccaaccaa cggtggtacg tagtttggcc catgcgagct gcgtatccat
     4201 atcccatgcc agaccatagg atttattacc catttcgcga tcagactgcg ggaaggtgct
     4261 ggtattataa tcacggctgg agccggtata ttttaaggtt aaatcgtggg taaagaggtc
     4321 gctggcaaac caggtaaatt tgctcaatgc agtatcgata acgtttttat actgtgcccg
     4381 accggcgaca atgccgtcat ttgaaacata atccgcgcgg gtgatatcag actggcggcg
     4441 cgataaaccg gcggtaacgc caaagttatc agcgagttcc tgattaaacg acaaggtata
     4501 aaagttcttt ttaaaatctg gagagtaata agtacttcct gaagaacctt gattaaatgc
     4561 gctcttgtta ttctcatcga tatgcgatgt taaccagtcc gaacgcgtag tgcgataacc
     4621 cagtttcacg ttgctatcat cagcgttgaa gcgtttgatc tttgcatcaa ttaccccgcc
     4681 attgaagcga ccgaattcaa ccggcacaaa actgtcataa agcgtcacat tgtccagtaa
     4741 gctgacatca agataatacc cctgtgacat cccgctaata ttggttgcac tactggcatc
     4801 ggactcattc gctgggttca ggttattagt ggcactaata ccatcaatca aataggcatt
     4861 ctggtagggc gacgcaccgt gaatagagat tttctccggg cgaatatctc cctggttcaa
     4921 cgaggtactt tgcgttgaat ccatgcgtac cgcagggttg gttctcagca gatcgctgat
     4981 attgccattc ccggtcggta aacgctcgat gctttcactg gtgtaatgcg tgttgccgtt
     5041 tacgggtaca gggacaggag catagaccgt catctcttca aaaaaatgca tgtacatgtt
     5101 tttatcatcg acggcctgcg ccacatcagc gccacataaa atgacgccag gaataagaac
     5161 tcgcttcatt tatttatatc tccgtacagc agggctttta ttgtttttat aaaaccgcgc
     5221 taatgtcaca aatatcatcg gccaggttcc agacaccggg ttgatgtgta accataatga
     5281 cgccgcttgt gggtagtttt tcacgcacta aacgcagtaa gcggatagcc tcttgttcct
     5341 caagatgaga ggtagtttcg tcaagaaata tccatttcgg acgtcgtaaa attaatcgtg
     5401 ccagggcgat acgttgtttt tcgccgctgg aaagaatatc tccccagcga tcgtggtcat
     5461 gaatacgcgc agccaatttc ccaagaccaa cctgatgcag tacttcgctc aacgatttat
     5521 cgtctacggg caggggaagt gctttacaaa taatctcttt cagtaagccg gttttgatta
     5581 acggtgtttg tgacacatac caactgtcag caggagaaga aatatcacct ttaaaccacg
     5641 gccagcagtg ggataatgtt ttaagcagtg tggtttttcc cgcaccagag taacctttca
     5701 gtaatagcca tttgcctggc ggaacatgaa agttcaggtt ctctaatatg atcttattat
     5761 caggggtacg aatactcgcg tcagccactt gtaccgcatg ttggcaattt ttaggcttat
     5821 tcgtagggcg ctgttcggtg agttgatgga actcatacaa gcgatcgata accgcagcca
     5881 gttcagcaag ttcgtcatat ttataaataa accagcttaa attgttcgat accagcataa
     5941 atgcctggcg cgatttcatc agtccgccca gattgatctg tccgctaata aactgcggca
     6001 ataacagaaa gtacggaaga acgctaagcg aacgcgaata aatattctgc cagtaatcaa
     6061 gccaccgctg acgattcatt aaacgatgcc agttctcttt aatggtatga aaattgtcgc
     6121 tcaactcctg gcgttgtaga gactcagcat tgcttagcgc aatcagttct gcctgtttat
     6181 tatgctgcac aagattagtt cgaaatgtcg cttcgctacg ttgtttttcc acattaagtg
     6241 gacgaatacg tttacccacc ttatgggtaa ataaagttcc accgatcaca atgagcacga
     6301 cggtatagac catatacccc tggatattcc attctgttcc accaacagtg aatgagagcg
     6361 tacccgcgct ttgccaaaga ataacggtaa aggtgatcag catgctaagt gactggatga
     6421 agccaaatga aagactgagc gttttgctga tcagtaagag aatgtcttca gcgatacgtt
     6481 ggtcagggtt atctgtattt ttatgctcgc cgtagatctg cgtgaagtag tagtttttat
     6541 ctgcaaacca ccgattaagg tagtaatctg ttaaccattc gcgccagcga atagtcagca
     6601 gtttaattaa ccatgttttg tttacggata ttaatacaaa gatccccagc agggcaggga
     6661 accaaaggac aagctgccaa agcttatcgg tttctttctg gcttagcgca ttgaaaaaat
     6721 cattattcca gtcgtttaac cagacctgaa ttttaaccac gccaaggatc atggcgagga
     6781 taataatgat taacaacact gaagttttat tattcttacg cagccaaaag ggcttcagca
     6841 gacagagata ctttgctatc aacatacaaa acgtaatggg aatggttatc attagcgaaa
//
LOCUS       DAZKFU020000242         6893 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZKFU020000242 DAZKFU020000000
VERSION     DAZKFU020000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.244"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            358..1842
                     /locus_tag="ACHOQQ_RS20130"
                     /old_locus_tag="pgaptmp_004026"
     CDS             358..1842
                     /locus_tag="ACHOQQ_RS20130"
                     /old_locus_tag="pgaptmp_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU4472218.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PNESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(2164..2766)
                     /locus_tag="ACHOQQ_RS20135"
                     /old_locus_tag="pgaptmp_004027"
     CDS             complement(2164..2766)
                     /locus_tag="ACHOQQ_RS20135"
                     /old_locus_tag="pgaptmp_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472219.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLMYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(2853..3059)
                     /locus_tag="ACHOQQ_RS20140"
                     /old_locus_tag="pgaptmp_004028"
     CDS             complement(2853..3059)
                     /locus_tag="ACHOQQ_RS20140"
                     /old_locus_tag="pgaptmp_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4472220.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            3818..3967
                     /locus_tag="ACHOQQ_RS20145"
                     /old_locus_tag="pgaptmp_004029"
     CDS             3818..3967
                     /locus_tag="ACHOQQ_RS20145"
                     /old_locus_tag="pgaptmp_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HGU4472221.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
     gene            complement(4382..4582)
                     /locus_tag="ACHOQQ_RS20150"
                     /old_locus_tag="pgaptmp_004030"
     CDS             complement(4382..4582)
                     /locus_tag="ACHOQQ_RS20150"
                     /old_locus_tag="pgaptmp_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472222.1"
                     /translation="MVIRKKKCWDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            4787..5356
                     /locus_tag="ACHOQQ_RS20155"
                     /old_locus_tag="pgaptmp_004031"
     CDS             4787..5356
                     /locus_tag="ACHOQQ_RS20155"
                     /old_locus_tag="pgaptmp_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGU4472223.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGIIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            5617..5994
                     /locus_tag="ACHOQQ_RS20160"
                     /old_locus_tag="pgaptmp_004032"
                     /pseudo
     CDS             5617..5994
                     /locus_tag="ACHOQQ_RS20160"
                     /old_locus_tag="pgaptmp_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
     gene            5998..6423
                     /locus_tag="ACHOQQ_RS20165"
                     /old_locus_tag="pgaptmp_004033"
     CDS             5998..6423
                     /locus_tag="ACHOQQ_RS20165"
                     /old_locus_tag="pgaptmp_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4472224.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLSVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            6670..6867
                     /locus_tag="ACHOQQ_RS20170"
                     /old_locus_tag="pgaptmp_004034"
     CDS             6670..6867
                     /locus_tag="ACHOQQ_RS20170"
                     /old_locus_tag="pgaptmp_004034"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4472225.1"
                     /translation="MPQDDFCIVNPGGCIVHFPANGRDIQDINAPDFPEQYQRLLARL
                     DYLTKPDAKASGQRNSGYSGF"
BASE COUNT         1742 a         1591 c         1813 g         1747 t
ORIGIN      
        1 gtgcaggaac tgtagtcttc tttattttat tcttcttcag gaggggggga ttcctgaagg
       61 cgttattccc ggtgttctgt ttatgattct tcccggcagg aaaccggtat ctgcaatacc
      121 cgggatgatg ctttttgttc gtacgtggtt gtgtaaaatt tgaatagtgg atgtgctctg
      181 aagggaagga ggggacacac agattcattt tgtctttctt cagcagcaaa acacactgtg
      241 gttattattt aaccattaaa gcaacaaaaa tttttacctc atattgattt gactgctatt
      301 tttgctatta atagccagaa ttgcagtact tttatatctg tgtgtagtga ggttactatg
      361 tcattcggtc agccttgtga tgagtttccc ctgagttcgc tgcctccact cattcgtgat
      421 gccgttattg aagctcagca aattacgcag gctccccttg ggcttgtggc agcgtctgcc
      481 ctgggcgctg tttcacttgt ctgccagaat ctgattgatg tctgcagact gaatacactg
      541 cgggggccgg tctctctttt tttcctgacg cttgccgagt ccggagaacg aaaaaccgca
      601 gtggacaaat tattaatgaa gcccctgtac cagcaggaga tgcagttata ttccagatac
      661 aaaagtgaac tggctgtctg gaaaaacaag gaagagcttt taaaggctca gaaaaaagca
      721 ttgttgtcaa aactgaataa agaactccgc aaaggcgcgg atgagtcaga aaccctcaga
      781 caactggaag tacttcagaa aaacagtgcg gaagaacctg tgaggtataa attcattttt
      841 aatgatgcca cgactgcggc aattaagaat cagctttgtg gtaaatggcg ttcagtgggt
      901 attatgtcag atgaagccgg tattatattt gatggatata ccctgtctga gttgcctttt
      961 attaacaaaa tgtgggatgg ctctgtattg tctgtggaca gaaagaatga acccgaacag
     1021 atgattgaaa atgccagaat gacgttatcc ctcatggtgc aacccggtct ttttgaccga
     1081 tatatggagc gaaaaggttc tgttgccagg gacagtggtt ttcttgcccg ttgtcttatc
     1141 agtaaacctg caaccacgca ggggaaacgc tttattaatg gagctgtgat tccgggagga
     1201 tcattaacgg catttcatga acgattaatg gaactggcca gaggcagtat tgaaaaatcc
     1261 agtgaggatg aacggtattg tctgcatttt tcgcctgaag cgcaaaaaat atttattgag
     1321 cactataatg ttctggagca ggatttatcc ccttccggac ccttaagccc gtttcgggga
     1381 catgtttcaa aaaaaacaga aaacattgca agaattgcag cgttatttca gtatttcagc
     1441 tatggtgaag ggaaaatctc tgccgatatc atgacgtctg ctgtcgtaat aagttcgtgg
     1501 tacacagatg aatataaaaa attatttgcg ttacctaatg aatctgaact gcagcagaag
     1561 gacgccgagg agctgttcga ctggctcatt gaggagtgcc gcggagaatg cccgccccgt
     1621 gtcaggaaaa attatattct ccagtgtggc ccgggccggt tcagaaacag gaaaaaactg
     1681 aacgcattgc tgaacattct tgaatctcag ttcaggcttt ctgtcgtgcc ggaaggaaaa
     1741 accatgtatg ttctgcttcc ccagatagcc agtttaaagt tgtctgatgt ctctggtatt
     1801 tttacctcgg gttatcacta caataagctg agagcgaagt aaccggtgag cagtttttct
     1861 ccattatgct tcagacttag atgattgatt attatgggga tttcatcacc gatattcaga
     1921 tgtatgaata tcggtgatga actaaagctc aggaactgcg tggtggtgat gaatcaacag
     1981 gcatataact gatacccgcc ggtcagaagt tccagcctgc gtctttcctg tctgcgtgcc
     2041 cgcagtccgg cgttcaggtg cagcgtgtga cagaacgcac ggagagcgaa acggcaggca
     2101 gacaggccgt tcagtcagcc ctggcaccct gcccggatac cagcttccct gaaacaactg
     2161 gcatcaggtg cgcttatcgt caaccgttgt gcggtatgcc aggagcacgt actgatccat
     2221 cagatccgtc agtgctgcgc tctgcttctg acggttgatg atccattcct ccagctcttt
     2281 ttctgatttc cgtttcagat tctccgtcca gtcgtcaagg cagtttccgg tcattaaatc
     2341 aagccagcaa agccagacca gtttgaggcg caactccgtg ccaccaaact gacggaagag
     2401 gctgatcagt ttatttgcca gcgaacgctg cctcagacgt tcacattcag acaggagaat
     2461 gccttccagc atcggatgat gaaccagcag atgttcactg gtgcataaat tcactatcag
     2521 atcatcatcc aggggcggcg tggtccgttt gtccggacca tacatcagcc ggtgcagact
     2581 ctggggtacg atggcaatct ccggtttttc accattgaat gacacccacc cgggcgtcag
     2641 attatggaag tagtagatat tcggatgaca cacgccccag gatacggcgt gccgggtgaa
     2701 gccggtcagc catttctggt agctttcgtg gagcagctcg taacgggtct gatactgtgg
     2761 tttcattttc atattcctca cgggggataa caggcagcag aacgccgccg gcaggccgga
     2821 gactgcgtct gctgcagatg aacggttaag aattacggac gggactgtgc aatacgcgcc
     2881 tgcagccagg cttccacttc gcttttcagc catcgggagc tgcggcccat tttgatgggc
     2941 gcaggaaagt caccatcctt gatgagtttg taaaaccact tatcggtcag gccggtcagt
     3001 tgagtgataa atgccatgtc gaccatctgg tcatccatca gtgaaactgg ggtgatcatg
     3061 agaatgttcc tccggatgtt gttaaaccag ccggtgtggt catgaaaaca acgcaccacc
     3121 ttcaccggca taacaaccgg ggagaaacgt aaaaaataat cgggaatgtg accgccagga
     3181 gataaacggg gaggttacgg tggctgagcc gtaccggtat gccgggtggt tgttacgaaa
     3241 cgatgcttcc gcacgcgcgc gcgataaaga agggatgatt ttcgtgagga agaggggaaa
     3301 actggcgttc tgataattgc agagttattt acggggcggt tacgatgggg cagaccgtgt
     3361 ccggtgggca ggtgacaggt atcaccgatg ggataagact gtctgattgg tattataaat
     3421 atataagaga gatcaaacac atgagatcaa atacatgagt tcatatatgt gaaataaaca
     3481 cggtgagtgt gatcagtggg catgatgaga acgcagtagc aacagactgc gctatccctg
     3541 atagctggcg tataacccgc gtatataaaa cgtatctgca atgtacgtga acagagtgca
     3601 ttaagcatcc gtctgagcgg taaacatatc cgacctggtg agtgacagtc tgagagaccg
     3661 gttaagtggt ggctcgggag taatgagttt ggggtgggta ccagaatgcc agtctggcgg
     3721 tatgctgaca gtatcacgtt aattgcagca taaagtgatg agaggctaat ggaagttaaa
     3781 accaaagcag ttccgtcgtt gttcatcccg ggagacgctg gaaaaagtga tttcccacac
     3841 gcgttataaa cttacctctg cggaactgga agccttcaac tctgtggttg atcactggct
     3901 ggcagaactg acaatgaaca agctttacga tcgtgttccc gcttcagtct ggaaatatgt
     3961 catctgagcg ttcggtaatc acaactgcat cgcgccaggt gttatccgct ttgtgattct
     4021 tattggctct tctcaggtta ttaaaagctg aatatgttcc tgttgtcggg aaccaggata
     4081 ataccgggaa cgttaagcgg tatagcgaca acaaagaatt cgaaatctaa agggaatggt
     4141 gccggaggcg tgggcggtaa tgggctgatt ttcaggtatt accggcttac agcgtgtggt
     4201 tgatattgag gggctgtgag tttcacgtat ttgtcagaaa cccagacgag ttttcctgta
     4261 tgagacgaaa taaggcagcg gcaatacctg gaaaaattta atatggtatg aaggagaacc
     4321 tgtcggttac gcttaatata cccggtacac tgtcagctca ggggatacat gaaccgttat
     4381 ctcaccagct acacagtata tacacactga cgctgacagt agtcagcagg accacaataa
     4441 tcagtgccag cagtatagta acgatacggt ctgtattccg gtaatagccg aaggggtcga
     4501 cagaaccaca atacgggcaa cacacagtat tatgagtaat cgcgtttcca cagtcccagc
     4561 acttcttttt gcgtatcacc ataactccct cctcacagcc ttatccgtaa tcggtttttt
     4621 acattaaaaa aacaacgctg aaaattcaac cgtcagtccg gagacgaccg ttcgggttat
     4681 ctcagagtgc ctgagatatt gcctggttgg agatcacttc agggactctg cgcatttttt
     4741 acggcgtggt taatccgtat gaacgaaacc atacggagat ttaaccatga cctacaaata
     4801 caaccccttc tggcagcaac gtattcgtga gacggtgcgg cacgcactgg atgttcatcc
     4861 ccgtctgacg gcattgcggg ttgatctgcg tctcccggat gtaccggcag caacggacgc
     4921 ggctgtgata tcccgcttca tcaatgccct gaaagcccga atcgacgctt accagaaacg
     4981 taagcatcgg gaaggtaaac gcgtgcattc ctcgaccctg cattacgcct gggcccggga
     5041 gttcggggag cttaaaggca aaaaacacta tcacctgctg ctgctggtca accgggatac
     5101 ctggtgtcgt gccggcgatt accgcgctcc ggggtcactg gccgggatta ttaaacaggc
     5161 ctggtgcagt gccctgggag tggatgccgg acgttatgac acgctggcac attttcctga
     5221 caagccagcg gtgtggctgg aacgcgatga tgaaaccggc tttcagcagg tgctggaacg
     5281 ttctggctat ctggcgaagg agcataccaa agctcgcggc accggtgagc gcaattttgg
     5341 ctgtagccgg agttgaacac gacaggcgca ccaatacccg gcaaaccaaa agtatgggtt
     5401 gttgtctggc ggcaacgatg tatccactcg cccactgact gttttttacg tcagtgggcc
     5461 attgtgtgac aaccacagtg ctgtaacagc aaccagcctg ctttacccaa actccagtcc
     5521 agattttaac tgaaccgcca ttcacaccct gaccacgctg cccgtacctg gacggctgtt
     5581 gcctgcgtgt cgtctggcgg ctgaggaata tatactatgt acgcaaaatc ctttctcgct
     5641 cttgatggca acggacgtct gacgggggcc cgtaccgcac agactgcgcc ttacgaccgc
     5701 tacacctgcc acctttgcgg cagtgcactc agataccacc cgcaatacga cactgaacgg
     5761 ccctggtttg aacacgctga cgaagggctg acggagcacg cgcagcaatg tccttatgtc
     5821 aggccggaac gcagagaaat acagttgatt aaacgtctgc agcaattcgt cccggatgcc
     5881 ttacccgtgg tgcgtaaagc cagctggcac tgcagacaat gtcaccacga ttattatggg
     5941 gagcggtact gcacacactg ccagaccgga cgcttcagtg aggagggggg tagcagaatg
     6001 aacggtataa aaacggggct ggggatcacg ccgggtgaac acattatcag tgccgactct
     6061 gcgctcagca gaaatatccg gcattgtttc tgcctttcct gccatggccg gttgattctg
     6121 cagacagatg ctcagggggc atggtttgag catgatttac atgccctgag tgagcaacag
     6181 aaggcggact gtgtggtgct gaatccggaa aagtcccacc cttacattga ggatatggcg
     6241 atgtttttat cgccattatc ggtcgtgctg gagtggcatt gtgtgatgtg tgagcagttt
     6301 tttcatggga agaagttctg tgaggcctgt ggtacgggga tttattgcag agcggtatgt
     6361 acgaagtctg tttacagcta ccagccagac ttgtttgagg actgcggagg tgcgagtact
     6421 taatccgaaa tccccgctct gcgcagggcg ttcagaggta ttgccaacct gtattttatc
     6481 cggtgtttcg cacccacccc gttcgtgaca caacggcgcg acaccggata tccgcagtcc
     6541 tgcttacctg aataaaaccc ggggtattga ttggtagtgt ggtgcggaat cgtaccgtga
     6601 tctcgcatcc gccgaattcg cactgtggtg tcaatcaacc atacgcgaga gtcctctccg
     6661 tatttatcag tgcctcagga tgatttctgc atagttaatc cagggggctg cattgttcat
     6721 ttccctgcta atggacgtga catccaggat attaatgctc ctgatttccc tgagcagtat
     6781 cagcgcttat tggcacgtct ggattatttg acgaagcccg atgctaaagc atcaggccag
     6841 cgtaactctg gctacagcgg tttttgacct gttgtaactg taaggtgatt tat
//
LOCUS       DAZKFU020000243         6745 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZKFU020000243 DAZKFU020000000
VERSION     DAZKFU020000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.245"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(150..635)
                     /gene="sixA"
                     /locus_tag="ACHOQQ_RS20175"
                     /old_locus_tag="pgaptmp_004035"
     CDS             complement(150..635)
                     /gene="sixA"
                     /locus_tag="ACHOQQ_RS20175"
                     /old_locus_tag="pgaptmp_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGU4472226.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVTS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(838..2982)
                     /gene="fadJ"
                     /locus_tag="ACHOQQ_RS20180"
                     /old_locus_tag="pgaptmp_004036"
     CDS             complement(838..2982)
                     /gene="fadJ"
                     /locus_tag="ACHOQQ_RS20180"
                     /old_locus_tag="pgaptmp_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGU4472227.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCESLVEMGARGESFWKTTATDLQ"
     gene            complement(2982..4292)
                     /gene="fadI"
                     /locus_tag="ACHOQQ_RS20185"
                     /old_locus_tag="pgaptmp_004037"
     CDS             complement(2982..4292)
                     /gene="fadI"
                     /locus_tag="ACHOQQ_RS20185"
                     /old_locus_tag="pgaptmp_004037"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGU4472228.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SNLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4473..4757)
                     /locus_tag="ACHOQQ_RS20190"
                     /old_locus_tag="pgaptmp_004038"
     CDS             complement(4473..4757)
                     /locus_tag="ACHOQQ_RS20190"
                     /old_locus_tag="pgaptmp_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGU4472229.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5129..6469
                     /gene="fadL"
                     /locus_tag="ACHOQQ_RS20195"
                     /old_locus_tag="pgaptmp_004039"
     CDS             5129..6469
                     /gene="fadL"
                     /locus_tag="ACHOQQ_RS20195"
                     /old_locus_tag="pgaptmp_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGU4472230.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDISGRSDLTGQRADADNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFNDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERTAGSLGPLLSQQLIAQGVPQSWTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFEGDYKSSINPNLNNILGSMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSLAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYHDKNWTFRTGIAFDDSPVPADKRSISIPDQDRLWLSA
                     GATYAFNEDASIDVGASYMHGQNVKFTEGEGPAAYTFKSEGKAWLFGTNFNYAF"
BASE COUNT         1700 a         1791 c         1596 g         1658 t
ORIGIN      
        1 tcagaaatta atagggaagc agaggaaaaa gtgcgatgaa ttctgtatcg aagcgtatca
       61 actccgtgaa aggttttgag tgaatatcta ctattgcaaa aagacaaatg cccgatgtaa
      121 gctgtacatc gggcattttt tcaaacgaat cagatagctt tcgccatctt caaattacat
      181 gggctcatct gccagttgaa ctcgcctttt ccgctttcat caagcgttac gctggcaatg
      241 gccgaggtgg taaacatagg cggcgtttcg cccggacata actcggcgac cagataccca
      301 actaacggta agtgggagat aaccaacacc gaagtaacac cttcattggt caacgcctgc
      361 aaataggcac tgaccagacc aacatcgccg cagggcgtta actccggcag aacttctgca
      421 ctggaaggca ggttcagaca atcccctact tcctccagtg tttgctcggc tcgcaggaac
      481 gggctcacca gaacgcgttc gatttccact ttttgacctt tcagccagtt cgccatcagg
      541 cgagattcgt cacaaccatt agtagtcaga ggacgaaccg aatcactggc ggcatcgagg
      601 gctgcgtcgc cgtgacgcat gataaaaact tgcatattgc accgcttttg ttaaccagat
      661 tcgcccgttt tctttcatct gaaaccagag aaagaaaacg gtggccggca ttgtgcctta
      721 tccattcacc gaatgaaacg ctgtttttta cctcaatggc gtaagtatag tcaatatgcg
      781 tttacatttt gaccaacaat ccgccattca gcacggattc atatagcttt gaccttctta
      841 ttgcaggtca gttgcagttg ttttccaaaa actttcccca cgcgcaccca tctcgactaa
      901 actctcgcaa ggggtaaaac gtgaaccata ctgcgtggcc agtcgttgca ttattgcaac
      961 cacttcgccc gcgccgagag aatcgatata gcggaacggt ccaccgagaa atggcggaaa
     1021 accaatgcca aataccgcac caatatcccc gtcacgcacg ctacggataa cctgctcatc
     1081 aacacaacgt actgcttcat tcagcatcaa catcacacac cgttcagcaa cctgcggtgc
     1141 ggagagtcgc ccctgccctt gtgcaccaat gagcgggtaa atggcaggat cgacctgttt
     1201 tttgctttta cgccctttct gaccataaag atagaaaccc cgaccatttt ttctgccttt
     1261 gcgatcgtcg ttcaaaattg aagaaacaac atttgcaggc gcgctaaaac gttctccata
     1321 agcggcttcc agtacaggaa taattttagt cccggtgtcg attcctacct catccaaaag
     1381 ttggattgga cctaccggaa aaccaaattt cactagcgcg gcatcaatgt gctctacccg
     1441 ttcaccttcg gtcagcatgc ggatagcttc attaatgtaa ggcgctaaga tgcgattgac
     1501 gtaaaacccg gctttgtcac gcacgacaat gggcgttttg ccctgttttt tcgccagttt
     1561 taccgtagtg gcgatggttt gcgctgatgt acccgcatga ggaataatct ccaccagcgg
     1621 cattttttcc accggactga agaaatgcag gccgataact tgctcaggtc gcgtggcgtg
     1681 agcggcgata tcaccaatcg gtaaagatga cgtgttcgaa gcaaagatgg tatgagcggc
     1741 gcaattttgc tcaacttccg ccaccatctg ttgttttaat tcgagatttt caaacaccgc
     1801 ttcaataatc agatcgcgat gggcaaagcc gcgatagtcc gtcgttccgg agattaatgc
     1861 cagctgtttg tcacgttcgc tggctttgag atgacgacgg cgaactttgc cctccagctg
     1921 atcccaactg tacttcagcg catgatttat gccctgcggg ttgatatctt taattctgac
     1981 cggaagcccc gctttacaag cagtgacata agcaataccg ccgcccatca agccaccacc
     2041 taaaatcccc acgctgttta atggcgcagg cggcgcatca ctgccgggat ctttcttcac
     2101 ttccgtactg gcaaaaaaga tactacgcaa agcttgcgat tgtggcgtca tcgccagttc
     2161 gccaaacgcc cgtgcttcag cgtcatagcc actgctggtg ccctgcgcta atcccgtttc
     2221 aacaacctcc aggatgcgtt ctgtcgccgg ataattgcct tgagttttgt gttctgtttt
     2281 cttgccgacc attttgaaca gcagcgcacg acctaacggc cccgccagaa tacgctcgcg
     2341 tacaggtaga gggcgggaag atgggcgatc cttctttgcc agctcaacag cggcttccag
     2401 cagaatggag tgcggaacaa cgtcatccac cagccccagc tttaatgcct gtttcgcccg
     2461 aagttgtttt ccggtgagga tcatctctaa tgctgtgctg acgcctatca gacgcggtaa
     2521 acgctgggtg ccgcctgaac cgggtaacaa tccaagttgt acttcaggca aaccgagcac
     2581 cgttttagga tcgtcagtac aaacgcgacc gtggcacgcg agcgccagct ccagcccacc
     2641 gcccaggcaa gcgccatgaa tagccgcgat aaccggaatg ggcaaagcat gaatctccgc
     2701 catcaactgt tggccctgcc gcgccagagc ttccgcttct tgcgccgttt tgcagttgcc
     2761 gatcatgttg atgtcagctc cggcgataaa gttgtctggt ttcgcggaaa taaataccac
     2821 gccgcgcaac tctttgtttt cgcggagttg cttaataatg gcgcgcacct gcgaggcaaa
     2881 ttccgccttc agggtattca ttttctcgcc cggtacgtcg atggtgatga tagcaatgtt
     2941 gtccagacga acattaagag taaacgctga tgccatttcc attattccgc ctccacaacc
     3001 attgccgcgc caagcccacc ggcagcacag gcggtcacta aaccaaatcc accgccgcgg
     3061 cggcgaagtt catgcaatgt ctgggttatc attcgcgcgc cggtcgccgc aaagggatgc
     3121 ccgtatgcaa tcgagccacc aagcacgtta aatttgctat cgtccacttc accggtggca
     3181 tgcgcacgcc ccagtacgtc gcgagcaaaa cgttcgctcc ccagtagctg aatattcgcc
     3241 agtgtctgag ctgcaaaggc ttcgtgcata tcgatcaatg tcagattgct catcgtcaaa
     3301 ccggcacgct ccagcgccag cggtgttgac caggctggac cgagcaacat gtcctgccag
     3361 acatcgatag cggtaaatgc gtagctgcgc agatatccca gcggtaccag ccctaattct
     3421 ttcgcccggg attcagtcat caggatcacc gccgctgcgc catcggtcag cggcgtactg
     3481 ttcgccgccg ttaccgttcc gtgtttacga tcaaacgccg gacgcagctt tgcgtaatca
     3541 gcaagcgagg aattaccgcg aatattgttg tcttccgcga gcagttgttt ataaggaggg
     3601 ataaaggcag tcatcacctc ttctttgagt tttccgtctg accatgcctg agcggcacgc
     3661 tgatgcgaac ggtgcgctaa tgcatcttgc tgttctcggg tgatgccgta ggttttcgcc
     3721 atttgctctg cagtgtcgcc catccgcaag ccggtagaat attctgctac cgcaggtggt
     3781 acgggcatta agtcgcgcaa acgcaggcga gagaagagtt tcagacgctg gctcattgtg
     3841 cgggctttgt tgacatcaac cagcacgcgc gccagttttt tactgacgcc aattggcaat
     3901 accgaagagg aatctgcccc accggcaatc cccgctcgaa tagttcccgc catcaggctt
     3961 tctgcgacgt ttgcaaccgc ctggaaactg gtagcgcaag cgcggctgac gctgtaagca
     4021 tcggtatgca cattcattcc ggtaccgaga acaatttcac gcgcaatgtt gggggcttca
     4081 ggcatttgta cgacctgacc aaagaccagt tgttcaatca cttcagcggg gatctcgctg
     4141 cgtgccagca gttcgcctac caccatcttc cccaaatcaa cagcaggaat gccatgaaaa
     4201 gccgtcgcct ggcgggcaaa aggcgtacgt aaaccgctaa caatggcgat acgatcgccc
     4261 tggcgggtaa ccagcggtaa aacctgaccc ataacactcc cctgtaaaaa aataaataaa
     4321 gtggtctgac ctgatcatag tcttaaccat ttttttacat ttagccaagc ggagaaaagg
     4381 gaaagtggga gctatgacac agagagaaaa gaagaagaga aaagaaaacg cccctgccag
     4441 caattgacag gggcgtacgt acccgagagg aattaacgca gacccagctg gaagatcagc
     4501 atttcagctt cgcaggcaaa agtgaaatcg atatccaagc gcacaccatc agactcttca
     4561 gtgaaagtcg gggagatttt gcacggttcg gattccacgc tacgggcttt ctcagtcagc
     4621 gccgccagcg tttgttctgc ttctgcgcgg tttgcaaaca cgcggctgta agacgcggtg
     4681 cagtcggagt tgtccataat ggtgccaaca tccatacagc agcaaaccgg ggtttcatca
     4741 gcactacatt tactcatcgt tgatttcctc tgtatgtgca cccaaggtgc cagataaacg
     4801 ttgtggatat tttacgcttc cggaaagtgc tgctccagtt gttaattctg caaaatcgga
     4861 taagtgaccg aaatcacact taaaaatgat ctaaaacaaa attcacccga atccatgagt
     4921 gcgccacctc caaattttgc cagctggatc gcgtttctta gatcatattt gaaaaaagat
     4981 agaaacatac ttgcaacatt ccagctggtc cgacctatac tctcgccact ggtctgattt
     5041 ctaagatgta cctcagaccc tacacttcgc gctcctgtta cagcacgtaa catagtttgt
     5101 ataaaaataa atcattgagg ttatggtcat gagccagaaa accctgttta caaagtctgc
     5161 tctcgcagtc gcagtggcac ttatctccac ccaggcctgg tcggcaggct ttcagttaaa
     5221 cgaattttct tcctctggcc tgggccgggc ttattcaggg gaaggcgcaa ttgccgatga
     5281 tgcaggtaac gtcagccgta accccgcatt gattaccatg tttgaccgcc cgacattttc
     5341 tgcgggtgcg gtttatattg atccggatgt agatatttct gggcgttccg atttaacggg
     5401 ccagcgtgct gatgcagata atattgcacc aactgcgtgg gtgcctaacc tacatttcgt
     5461 agcacctatt aacgatcagt ttggttgggg ggcttctatt acctcgaact atggtctggc
     5521 cacggaattt aatgataact acgctgctgg aatctatggc ggtaaaaccg atctgcagac
     5581 gatcaacctt aaccttagcg gagcctaccg ccttaacaat agctggagtt ttggtcttgg
     5641 ttttgatgct gtatacgcta aggcaaaaat tgagcgtact gccggttcac tcggtccatt
     5701 gctttcccaa caactgatag cgcaaggcgt gccacaaagt tggacaaacg gcatcaatac
     5761 accggattcg caaattgctc atctcaaggg tgatgaatgg ggattcggct ggaacgccgg
     5821 tattctttat gaactggata aagataaccg ttggggccta acctatcgtt cagaagtgaa
     5881 aattgacttt gaaggagatt acaaaagctc catcaaccca aatctgaaca acattttggg
     5941 tagcatgggt ttaccatatg gcacaatggg tgcaacccaa aatgggtcct taacgctgaa
     6001 tttacctgag atgtgggaaa tttctggcta taaccgtgtc gcaccacaat gggctatcca
     6061 ctatagtctg gcgtatacca gttggagtca attccaggaa ctgaaagcga aaggagactc
     6121 tggtcaaacg ctgttctata aagacgaagg tttccgggat gcttaccgta tcgcgttagg
     6181 tacaacctac taccacgata aaaactggac cttccgtacg ggtattgcat tcgatgacag
     6241 cccggttccg gcagataaac gttctatctc tatcccagat caggatcgtc tgtggttaag
     6301 tgcaggtgcg acttatgcat ttaatgaaga tgcatccatt gacgttggtg catcttatat
     6361 gcacggtcag aatgtgaagt ttactgaagg tgaagggcca gcagcatata cctttaaatc
     6421 tgaaggtaaa gcctggctgt tcggtactaa ctttaactac gcgttctgat aacgcgttcg
     6481 cctggataaa gtcacctgct atgcaggtga ctttaactcc cccacttcac cgaaagtagt
     6541 ttcctcattg cttgcctcct gagttttgca aacagcctga tggcagcttg ccctttcaaa
     6601 tcagtaagtg gtatccacag acaaacctga agggaaacgc atattttcag gcgttctgct
     6661 cgtccttctc aaagagttta cttttctgaa tttccaggat actcaacccc ctggctattg
     6721 tgcgctcata cactcaaatt aaaga
//
LOCUS       DAZKFU020000244         6650 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZKFU020000244 DAZKFU020000000
VERSION     DAZKFU020000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.246"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..538
                     /locus_tag="ACHOQQ_RS20200"
                     /old_locus_tag="pgaptmp_004040"
     CDS             <1..538
                     /locus_tag="ACHOQQ_RS20200"
                     /old_locus_tag="pgaptmp_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4472231.1"
                     /translation="ALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQTNSVVLLP
                     TVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDASTGVMGNR
                     DTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGARYFVIDSA
                     AATAGKITAVAEYTLSYL"
     gene            624..1325
                     /locus_tag="ACHOQQ_RS20205"
                     /old_locus_tag="pgaptmp_004041"
     CDS             624..1325
                     /locus_tag="ACHOQQ_RS20205"
                     /old_locus_tag="pgaptmp_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4472232.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVVIRAAELLSNGKKVPLANSVMIAPQSTTEWTLRSGISVARGALIHLV
                     TVNDYGVNVTSEHAL"
     gene            1378..3900
                     /locus_tag="ACHOQQ_RS20210"
                     /old_locus_tag="pgaptmp_004042"
     CDS             1378..3900
                     /locus_tag="ACHOQQ_RS20210"
                     /old_locus_tag="pgaptmp_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4472233.1"
                     /translation="MNKSVVSISAAMLVLLYQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVATTNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDFTWQHGLTNTLTFNSG
                     SRIADGYQALMLGGVYGSSLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGVTFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSEKQVAP
                     VAGAAVKVVFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            3916..5250
                     /locus_tag="ACHOQQ_RS20215"
                     /old_locus_tag="pgaptmp_004043"
     CDS             3916..5250
                     /locus_tag="ACHOQQ_RS20215"
                     /old_locus_tag="pgaptmp_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4472234.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWATCSNTRVGQTEDGRSALI
                     EFGKINLTDTYFAPVGSLLATTVVPSTNYTSGGASGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFHNAGGPDGLSDVYATWFAFVGLKQTMAGVTIGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTAGATSYCGNTNTDGDGVGYAKPSGTPYNCTQPNAY
                     IQLSGTSGIPFAHDEVGEDSADHYDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAYPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5247..5936
                     /locus_tag="ACHOQQ_RS20220"
                     /old_locus_tag="pgaptmp_004044"
     CDS             5247..5936
                     /locus_tag="ACHOQQ_RS20220"
                     /old_locus_tag="pgaptmp_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4472235.1"
                     /translation="MKSVLLLLTVLFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(6082..>6650)
                     /locus_tag="ACHOQQ_RS20225"
                     /old_locus_tag="pgaptmp_004045"
     CDS             complement(6082..>6650)
                     /locus_tag="ACHOQQ_RS20225"
                     /old_locus_tag="pgaptmp_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4472236.1"
                     /translation="DITKPDAACTIINRCLERFGQLDILVNNAVICNINRPIDFNRND
                     WDPMINLNLNAAFDMSQAALNIFVPQRKGKVINMCSVLSFHGGRWSPGYAATKHALAG
                     LTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKGRIPAQRWGRAQDLM
                     GAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1750 a         1661 c         1594 g         1645 t
ORIGIN      
        1 agctctgctg gcagctatcg cattaccttt ttctaccatg gctgcaccga cagtcacttt
       61 tcagggtgaa gtaaccgatc agacctgttc cgttaatatc aacggtcaaa ccaattcagt
      121 agtattgctg ccgaccgtag ccatggctga cttcggtgca actttagctg atggccagag
      181 cgcaggccag acgccgttta cagtttctgt gtctaactgc caggctccga ctggtgcaga
      241 tcaggcaatc aacaccactt tcctgggcta cgacgttgac gctagcacgg gtgttatggg
      301 aaaccgtgat accagcagcg atgcggcgaa aggctttggc attcagttaa tggattccag
      361 cacttctggt aacccagtaa ctctggctgg cgcgactaac gtaccgggtt tgaccctgaa
      421 agttggcgat actgaagcca gctacgactt cggtgcgcgt tacttcgtta tcgatagcgc
      481 agctgccact gccggtaaaa ttaccgctgt cgcagaatac accctgagct acctctaatt
      541 aatctactgg ctggcagtaa atgccagcca gtatttcttc gcacaattaa tagtgatttt
      601 tcatggacat ggaaaagata agaatgaaca tttttgcata tttactggta cttgtatttt
      661 ccatgagcat gagcagcagc gcgtttgcca gcgtggtaat gaccggaacc cgtattattt
      721 tccctggtga cgcaaaggaa aaaaccatcc agttgcgaaa taccagcgat cagccctata
      781 tcattaatat ccatgttgag gatgaacgtg gttctgacaa gaatgtaccg tttatgccaa
      841 ccccgcagac atttcgcatg gaagctgccg caggtcaggc gttacgcctg ctctacactg
      901 gtaataattt accgcaggat cgcgagtctg ttttctggtt tagtttcagt caactacctt
      961 atctgaataa gaatgataaa agtcagaacc agctcatcct ggccctgact aatcgagtca
     1021 aaattttcta tcgtcccagc tcgattgtcg gtaaatccag tgacgcaccc aaaaacctga
     1081 cttaccaggt aaaacagaac cgcattgaag tgacgaatcc cacgggctat tacgtcgtga
     1141 ttcgcgccgc tgaactgctt agtaatggta aaaaagttcc cctcgcgaat tccgtcatga
     1201 ttgctcctca aagcacaact gaatggacac tacgctctgg catcagtgtc gctcgcggtg
     1261 cgctgattca tttagtgacc gtcaacgact atggcgtaaa tgttacgtct gagcatgcct
     1321 tataacctct ttctcctttt ttgagcggta tcctttttca cgcacgagta cgtcgagatg
     1381 aataaatccg ttgtgtcaat ttctgcggca atgttggttt tactttacca accggtcatg
     1441 gggagcgaaa tctcacccgc aacaccgtca gatgaagaca actacacctt tgacccgcaa
     1501 ctcttccgcg gcagcagatt tagtcagtcg tcattagcaa aactgacaac acgtgagtct
     1561 gttgcaccgg gcaattataa aatggatatc tacaccaaca ataagttgtc aggcagttgg
     1621 aatgtcacgt ttaaagaagc cgctgatggt cgcgttctgc cctgcctgac gcctgaagtc
     1681 gcggacgcga taggcctcaa aacaggggaa gataaggggg aaaaagatcc tgtctgtacg
     1741 tttgctaagg aactcgctcc cggcatcacc agccagacac agttgtcaca attgcgcctg
     1801 gacttgtcgg tgcctcagag tcaattgatt agtcgccctc gcggctatgt tccccctagc
     1861 gagctggata ccggagcatc gctggcgttc atgaattata ttgccaacta ttacaacgtt
     1921 gcctattcag ggcagaatgc tcatagccag cgttcgctat gggcatcatt taatggcggc
     1981 attaaccttg gtgcctggca atatcgtcag ttatccaaca tgacctggga taacgacaaa
     2041 gggaatcagt ggaacaatat tcgtagctat ttgcaacgcc cgctgcccgc cataaatagt
     2101 caattaatga tggggcaact tatcaccagc ggaagatttt tctctggact cagttatcac
     2161 ggcgttagtc tcgcgaccga tgaacgtatg ctgccggact ccatgcgcgg ctatgcgccg
     2221 actattcgcg gcgtggctac aacaaacgcc agagtctcgg taatgcaaaa cggtcatgaa
     2281 atttatcaga ctaccgtggc tccaggccct ttcgaaataa acgacctata ccccaccagc
     2341 tacagcggcg atctggatgt caccgttacg gaagctaatg gcgcagtcag tcgtttcagt
     2401 gtcccctttt cagccgtacc agaatcgatg cgtccaggaa cttcccgtta taacgttgaa
     2461 gtaggtaaaa cgcaggatag cggtgatgac tcgatgtttg gtgacttcac ctggcagcac
     2521 gggctgacta atacgctaac atttaacagt ggctcgcgta ttgctgatgg ctaccaggcg
     2581 ctgatgcttg gcggagtcta tggcagttcg ctgggggcat ttggggcaaa cctcacttgg
     2641 tcccatgcgc gtgttcccga aagcgaagca cagagtggtt ggatgtcgca attaacctgg
     2701 agtaaaactt tccagcctac ttcaaccacc gtctccctgg caggttatcg atactctacc
     2761 agcggctatc gtgatctggc tgatgtgctg ggagagcgtc atgctgccag taataaacag
     2821 tcatgggact ccagccagtg gcgtcaacag tcgcgcttcg atcttacgtt aagtcagagc
     2881 cttgcgaatt acggcaacct gtttgtgtca ggttcaacac agaactaccg tggcggcaag
     2941 agccgtgata cacagcttca gttaggttac agcaatagct ttagccacgg catcagtatg
     3001 aacctttccg tcggacgcca aagaatgggc ggctataaag acaattctga tgatatgcag
     3061 acggtaacgt ccctttcatt ctcattccca cttggcggca atggacctcg tgtaccaagt
     3121 cttagcaaca gctggaccca ttcaactgac ggtagctcgc aattacaaag ctcgctaacc
     3181 ggaatgcttg atgaagcaca gaccaccaac tacagcctga acgtcatgcg cgatcaacaa
     3241 tataagcaga cgacgcttag cggaaacatg caaaaacgtt tttcacaaac taccgtcgga
     3301 ttgaacgcat cgaagggcca ggattactgg caggcgtccg gaaacgtaca aggcgcgatg
     3361 gccgtccatg gcggcggcgt cactttcgga ccttatctgg gtgaaacgtt cgccctggtc
     3421 gaagcaaaag gcgcagaagg tgcaaaagtc tataactcca gtcagctgga aattaatgac
     3481 agtggctatg cgcttgttcc ggcagtaacg ccctatcgct acaaccgtat atctctcgat
     3541 ccacaaggaa tggatggcga tgccgaactg gtcgacagtg aaaaacaggt agcaccggtt
     3601 gcgggtgcgg cggtgaaagt agtcttccgt acccgtcctg gtaaagcgct gctgattaaa
     3661 tcccgcatgg cagatggttc ggaactgcca atgggagccg atgtgctgga tgagaataat
     3721 actgttgtcg gtatagccgg tcaggggggg caaatttacc tccgcacaga acagacaaaa
     3781 ggccatttgt cagttcgctg gggtgaaggc gctaacgata gctgccagtt gccctttgat
     3841 atcagcggga aagacagcaa tagccctatc atccgcctga atgaaacctg tcagtcttga
     3901 tacagagaaa acccgatgtc taaatcgact tttttacaca ttctgattag ctccatcata
     3961 ttggtggcac taatacaatc atccgcctgg gccacttgtt ctaatacacg tgtcggacaa
     4021 acggaggacg gaagatcggc actcattgag ttcggaaaaa ttaacctgac cgacacctat
     4081 tttgcgccag taggctcact actcgcgaca acagtcgtac cttccactaa ctacacatca
     4141 ggtggcgcgt caggaagctc cgtactgtgg gaatgtgacg caacagattt gccaaacatc
     4201 tattttctgg tcgcaaccaa tggcgacgac cgcgtgggtg gctttcacaa tgcaggcggg
     4261 cctgatggtc tgagtgatgt ctatgccacc tggtttgctt ttgtcggtct caagcagact
     4321 atggcgggcg tgacgattgg tcgttactgg aaaaaagtgc ccatcactag ttatgccact
     4381 cagggaacta aaatccagat ccgcttacag gatatccctc ctcttcatgc tgagctttac
     4441 cgtattagta cgctgcctga taccgctggc gcaaccagtt actgcggtaa taccaatact
     4501 gatggcgacg gtgtggggta tgctaaaccc tccggcacac cttataactg cactcagcct
     4561 aatgcctata ttcagctttc cggtaccagt ggtattccat ttgctcatga tgaagtgggc
     4621 gaggacagtg cggatcacta tgatttttgg ggtgctgata atggttttgg ttacggaatg
     4681 cgttcggcca atcgactcta caacaatgcc acctgcgttg cccgcagcgc cacgccgtta
     4741 gtattgctgc cgacaattgc agaagcacaa ctgaatgcgg gcatggaaag taccggtaat
     4801 tttaatgtcc gcgtcgagtg tagtaactcg gttcaatcag ggattagcga tactcagact
     4861 gcattaggaa tccaggtgtc tgaaggtgca tatacagcgg cgcaaaaact ggggattatc
     4921 aatagcaacg gcggcgtcag cgccctagtc tctgataatt atgacgcagc agagatggca
     4981 aagggcgttg ggatctacat ctcaaacagt gcttaccccg atacggcgat gacgctggtt
     5041 ggtcaaccgg gcatcgcgaa gttaaccccc ggaggaaatg cagcggggtg gtatcctgta
     5101 tttgaagggg caacattaga aggtgcgact caccccggat actccagcta tagttactct
     5161 tttatcgccc ggttgaagaa actgccaaat cagacagtca gtgcgggaaa agtgcgggca
     5221 acggcgtata tattggtgaa aatgcaatga aatccgtttt gctgctctta accgtcttgt
     5281 tcgtacagtc agtggaagcc ggcatcatgc cggcttcttc acggattatt tatcgcgatg
     5341 gcgatcggga aaagacactg atgctggtca ataccaatga ctatccggta ctggtgcaaa
     5401 gttgggtaga taatggtgaa ggttccccag aagctgcttc tccccccttt gtggtgctgc
     5461 caccggtttt ccgcctggct gcgagacgaa cccaggggtt gcggattatc tataatcatg
     5521 acccacttcc ccaggatcgc gaatctgcat tctggttaaa tctttatgaa atgccgccag
     5581 aaactcccca tcaagatccc acacatttaa cgctggcaat gaatacgcaa cttaaaatct
     5641 tttacaggcc aaaatctgta actatgacgc cccaagaagc tatgcgtaaa ataagttggc
     5701 atctgggtaa cgacggcagc agtagctata ttgagcttaa taacccaaca ccactgaata
     5761 tctctttcac ctccatcagc attggggcta caaaggtgca gcaagaaagc gatatgatga
     5821 taaaaccctt cagtcaacga cggtactggt tagcaagtaa taacgttagt ggtgtggttg
     5881 cggcgacata tctcgatgat aatggtgcac agataaaaca acaattagcg caataatgct
     5941 ctgagtggta tgtaaactca tcgcgatggt tcaacgctaa cgaataacca ctatacccac
     6001 ctgaagtacc ggaagtggag aaatgacaaa atctctccac tccattttcc agtcaaaaca
     6061 cctcaacgca gccaaaaatc gttaacgaat tgaatatcca ccatcaatga ctaatagttg
     6121 tccattgaca taatctgaag cggcactggc gaggaacacc attgcgccca taagatcctg
     6181 tgcccttccc cagcgttgag ccggtatacg cccttttatt aactctttta ttttagggtt
     6241 attataaata atagcggtca tttcactcac ataatatcct ggggcgatac cattaatctg
     6301 aatattatat tccgcaaaat catcggcata cgctttggtc agccccgcta aagcatgttt
     6361 agtcgccgca taccctggcg accaacgtcc cccatgaaat gacaatacgg aacacatatt
     6421 aataactttc ccttttcttt gcgggacaaa tatattcaat gcggcctggc tcatatcgaa
     6481 tgcagcattc aggttgagat taatcatcgg atcccagtcg tttcgattga aatcgatagg
     6541 acgattaatg ttacaaatca ccgcgttatt gaccagaata tctaactgac cgaaacgctc
     6601 cagacaccta ttaataatgg tgcatgctgc atcaggtttt gttatgtcta
//
LOCUS       DAZKFU020000245         6631 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZKFU020000245 DAZKFU020000000
VERSION     DAZKFU020000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.247"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            183..425
                     /gene="yjbE"
                     /locus_tag="ACHOQQ_RS20230"
                     /old_locus_tag="pgaptmp_004046"
     CDS             183..425
                     /gene="yjbE"
                     /locus_tag="ACHOQQ_RS20230"
                     /old_locus_tag="pgaptmp_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGU4472237.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            539..1177
                     /locus_tag="ACHOQQ_RS20235"
                     /old_locus_tag="pgaptmp_004047"
     CDS             539..1177
                     /locus_tag="ACHOQQ_RS20235"
                     /old_locus_tag="pgaptmp_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGU4472238.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1174..1911
                     /locus_tag="ACHOQQ_RS20240"
                     /old_locus_tag="pgaptmp_004048"
     CDS             1174..1911
                     /locus_tag="ACHOQQ_RS20240"
                     /old_locus_tag="pgaptmp_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4472239.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1911..4007
                     /locus_tag="ACHOQQ_RS20245"
                     /old_locus_tag="pgaptmp_004049"
     CDS             1911..4007
                     /locus_tag="ACHOQQ_RS20245"
                     /old_locus_tag="pgaptmp_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4472240.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGVDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4054..4332)
                     /locus_tag="ACHOQQ_RS20250"
                     /old_locus_tag="pgaptmp_004050"
     CDS             complement(4054..4332)
                     /locus_tag="ACHOQQ_RS20250"
                     /old_locus_tag="pgaptmp_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472241.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4333..4476
                     /locus_tag="ACHOQQ_RS20255"
                     /old_locus_tag="pgaptmp_004051"
     CDS             4333..4476
                     /locus_tag="ACHOQQ_RS20255"
                     /old_locus_tag="pgaptmp_004051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472242.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4602..5012
                     /gene="psiE"
                     /locus_tag="ACHOQQ_RS20260"
                     /old_locus_tag="pgaptmp_004052"
     CDS             4602..5012
                     /gene="psiE"
                     /locus_tag="ACHOQQ_RS20260"
                     /old_locus_tag="pgaptmp_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGU4472243.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5056..6531)
                     /gene="xylE"
                     /locus_tag="ACHOQQ_RS20265"
                     /old_locus_tag="pgaptmp_004053"
     CDS             complement(5056..6531)
                     /gene="xylE"
                     /locus_tag="ACHOQQ_RS20265"
                     /old_locus_tag="pgaptmp_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGU4472244.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1637 a         1688 c         1777 g         1529 t
ORIGIN      
        1 actatgattt ttaatgttat ttatatataa gttgtagctt atatttgata ttcattccaa
       61 ttatcctaaa accccatctc taattccccg cgccgtaatt cgcatgcttt agttgtgtat
      121 actcgatccc gcccgaaatg tttttgggta aatctccatt cattcaatga agggaaattg
      181 ttatgaaaaa agttctgtat ggcatttttg ccatatctgc gcttgcggcg acttctgcgt
      241 gggctgcacc tgtacaggtg ggcgaagcgg cagggtcggc agcaacgtcg gtttcggcgg
      301 gtagttcctc cgcgaccagc gtcagcaccg taagctcggc ggtgggtgtc gcgctcgcgg
      361 caaccggtgg cggtgatggt tctaataccg ggaccacaac aaccacgacc accagtaccc
      421 agtaataaag tatgtatccc caaaataatt cgagtcatcg catctgtggc ttgaagtatg
      481 aagggattta ccataaccac actccggtgt ggttattctg cccctctgga gaagagtcgt
      541 gaagcgacct gcattcattc ttatctgcct gctattacag gcctgttcag ccacgactaa
      601 agagctgggc aattcactgt gggacagtct gttcggtacg ccaggtgtac agctgacgga
      661 cgatgatatt caaaatatgc cctacgccag ccagtacatg cagcttaatg gcgggccgca
      721 gttgtttgtg gtgctggcct tcgctgaaga cggacaacaa aaatgggtca ctcaggatca
      781 ggccactctc gtcacacaac atggtcgtct ggtgaagact ttgcttggcg gcgacaacct
      841 gattgaagtg aataaccttg ccgccgaccc gctgattaaa cccgcacaaa ttgttgatgg
      901 tgcaacatgg acgcgcacga tgggctggac cgagtaccag caggtacgct acgccaccgc
      961 acgctcagtc ttcaaatggg atggcaccga taccgtcaaa gtcggcagcg atgaaacccc
     1021 ggttcgcgtg ctggacgaag aagtctccac cgaccaggcg cgctggcata accgctattg
     1081 gatcgacagc gaagggcaaa ttcgccagtc ggaacagtat ctcggcgcgg attatttccc
     1141 ggtgaaaacc accctcatca aggcggcaaa acaatgatca aacaaacttt tgtcgcgttg
     1201 cttttgagtg tgggagcgtc atcggtcttc gcggcaggaa ccgtcaaggt gttcagcaat
     1261 ggcagcagtg aggccaaaac actaacaggc gcagagcatt taatcgatct ggtaggccaa
     1321 ccgcggctgg caaacagctg gtggcccggt gcggtgatca gcgaagagct ggcaacggcg
     1381 gcagcattgc gtcagcagca ggcgttgctg acacggctgg cagaactggc cgcagactcc
     1441 agcgccgacg atgccgctgc gattaacgcc ttacgccagc aaattcaggc gttgaaggtg
     1501 acgggcaggc aaaaaatcaa tcttgatccc gatatcgtcc gcgttgctga acgcgctaac
     1561 ccgccgttgc agggcaacta cacgctgtgg gtcggaccac caccgtccac ggtcacgttg
     1621 ttcgggctta tcagccgtcc tggcaatcag ccattcactc ccggtcgcga cgtggcgagc
     1681 tatctctccg ggcaaagtct gcttagcggt gcggatcgta gctacgcgtg ggtggtttac
     1741 ccggacggac gcacacaaaa agcgccggtg gcttactgga acaagcgtca cgtagagccg
     1801 atgcccggca gcattattta tgttggcctc gcggactccg tctggagtga gatgcctgat
     1861 gcccttaacg ccgacattct tcagactctg acgcagcgga tacctcaata atgaaaaaaa
     1921 gacatctgct tagcttactg gcgctgggca ttagcacagc ttgctacggc gaaacatatc
     1981 ctgcgcccat tggtccgtcg cagtcggatt tcggtggcgt aggattatta caaacgccca
     2041 ccgcgcgtat ggcacgggaa ggggagttga gtctgaacta tcgtgataac gatcagtacc
     2101 gttattactc agcttcagtg caactcttcc cgtggctgga aacaacgctg cgctacaccg
     2161 acgtgcgcac ccggcagtac agcagcgtcg aagcgttctc tggcgatcaa acgtataaag
     2221 ataaagcctt cgatctcaaa ctgcgtttgt gggaagagag ttattggctg ccgcaagtgg
     2281 cggttggtgc gcgggatatc ggcggtacgg ggctgtttga tgcggaatat cttgttgcca
     2341 gcaaagcctg ggggccgttc gattttacgc tcggcctggg ctggggatat ctgggcacca
     2401 gcggtaatgt gaaaaatccg ctctgttcag ccagtgataa atattgctat cgcgataaca
     2461 gctacaaaca ggcgggatct atcgacggta gccagatgtt ccacggtcct gcctcactgt
     2521 ttggcggcgt ggaataccag acgccctggc aaccgctgcg cctgaaactg gagtatgaag
     2581 gcaataatta tcagcaggat tttgccggga agctggagca aaaaagtaag tttaacgtcg
     2641 gggcgattta tcgcgttacc gattgggccg acgttaacct tagctatgaa cgtggcaaca
     2701 cctttatgtt tggcgtcacg ctgcgcacca actttaacga tctgcgcccg tcttacaacg
     2761 ataacgcccg cccgcaatat caaccgcagc cgcaggatgc cattttgcag cattcggtgg
     2821 tggcgaatca gttaacgctg ttgaaataca atgccggact tgccgatccg cagatccagg
     2881 cgaaaggcga tacgctgtat gttaccggcg agcaggtgaa atatcgtgat tcgcgcgaag
     2941 ggatcatccg tgctaatcgg atcgtgatga atgatctgcc ggatgggatc aagacgatcc
     3001 gcgttacgga aaatcgcctt aacatgccgc aggtgacgac ggaaacggat gtcgccagcc
     3061 tgaaaaatca tctcgccgga gagccgttgg gccacgaaac gaagctggcg caaaaacgcg
     3121 tcgagccagt ggttccgaag tccaccgagc agggctggta tatcgacaaa tcacgctttg
     3181 atttccatat cgatccggtg ctgaaccagt cggtcggtgg cccggaaaac ttttacatgt
     3241 atcagctcgg cgtgatggga acggcagatt tgtggctgac ggaccatctg ctgaccaccg
     3301 gcagcctgtt cgctaacctt gccaacaact acgacaagtt taactacacc aatccgccgc
     3361 aggactcgca cttaccgcgc gtgcgtaccc atgtgcgcga gtatgtgcag aacgatgtct
     3421 atgtgaataa cctgcaagcc aactacttcc agcatctggg caacggcttc tacggtcagg
     3481 tctacggcgg ctatctcgaa accatgtttg gcggtgcggg ggcagaagtg ttgtatcgcc
     3541 cgctggacag caactgggcg tttggtgtgg atgccaacta cgttaaacag cgcgactggc
     3601 gtagtgcaaa agatatgatg aaattcaccg actacagcgt gaaaaccgga catctgaccg
     3661 cctactggac gccatctttc gctcaggatg tgttagttaa agccagcgtc gggcagtatc
     3721 tggcagggga taaaggcggc acgctggaga tcgccaaacg ctttgatagc ggtgtggtgg
     3781 tgggtggcta tgccacgatc actaatgttt cgaaagagga gtacggcgaa ggggacttca
     3841 ccaaaggcgt gtatgtctct gtaccgttgg atctcttctc gtctggcccg acacgcagcc
     3901 gtgcggcaat tggctggacg ccgctgacgc gtgacggtgg tcagcaactt gggcgtaagt
     3961 tccagttgta tgacatgacc agcgaccgta gcgtcaattt ccgctaagtc atgggaaagg
     4021 tgccagtttt cgcactcact ggcaccttca ttcttaatgg cctgtcgagc catcaggaac
     4081 gcagttcacc cccggtttgc gatctggtgt gtaaaggcga aaacattctg tcttcctgtc
     4141 cgtcgatatc ggttgcaggt ctggttgcct gagcgactgg gccatcattg ggctggagac
     4201 attgctttgc ggtaggtcgg ctttatcagc agtaccgagc ggaccagcat aagcaggaag
     4261 aacagagact gataacatca aagcagcaaa ataaggcttc atttttacca cctttatcag
     4321 gttacgtttc atttgttcca gaggaacatt gtcgattttt cgcgcattgc tggtggctgg
     4381 gaatcacctg aatgggtgat ttttgaatta ccggctttgg tgcggtttgt cttgccggat
     4441 gcgccgccag gcgcggctta tccggcctac gggtaggtat atccggcttt ggtggaggcg
     4501 cgctccaaat ccaggttgaa caaaacatac acaaaaaata tagatctccg tcacattttt
     4561 gcgttataca ggaagctcgc cactgtgaag gaggtactgc tatgacgtca ctctctcgtc
     4621 cgcgcgtgga gtttatctcc accattttgc agaccgtact caatcttggc ctgttgtgcc
     4681 tcggcctgat tttggttgtc ttcctcggca aagaaacggt gcatctggct gatgtgctat
     4741 tcgcgccaga acaaaccagc aaatatgagc tggtagaagg actggtggtt tactttctct
     4801 atttcgaatt tatcgcgctg attgtgaagt actttcagtc cggttttcac ttcccgctac
     4861 gttactttgt ctatattggg atcaccgcca ttgtgcgctt gatcatcgtc gatcataaat
     4921 cgccactgga tgtgttgatc tactcggctg cgatcctgtt actggtgatc accctgtggc
     4981 tgtgtaattc gaaacggctg aagcgggagt aaaaagacag cacgccgaac tggcgcggcg
     5041 tgctggacag gaagattaca gcgtagcagt ttgttgtgtt ttcttcgttt ccggttccca
     5101 gagggcttcc agctcctcaa gggttttacc tttggtttcc gggacaaatt tccacataaa
     5161 tagtgctgcc agaacgccca tacacccgta aatccagtag gagaaaccgt tgtggaaatg
     5221 ggccaccagc caggagtttt tgtccatcat cgggaaggtc caggagacga agtagttcgc
     5281 cagccactgg gccgccaccg cgattgccag cgctttacca cgaatagcat tcgggaagat
     5341 ttccgacagc agtacccagc ataccggacc ccaggacatg gcaaaggcgg caacatagaa
     5401 cagcatcgac agaagcgcca caatacccgg tgcctgagtg taaaacgcgg taccgaggct
     5461 aaacataccg attgccattc cgagtgcgcc gataatttgc agtggcttac gaccaaattt
     5521 atccaccgtc ataattgcca gaacggtgaa ggtgaggttg ataactccga caataatggt
     5581 ctgcaacagc gcgatatccg tgctggcccc cagcgttttg aacacttccg gcgcgtagta
     5641 cagcaccaca ttgatgccga caaattgctg gaagatggag agcataacgc cgatgacaat
     5701 cacgcccacg ccaaacatca gcagacgacc accggttttg cggccatgat ccagggagtg
     5761 tttaatttcc tgtactgcct gagttgcaag cgtgttgccc ataattttgc gcaggatacc
     5821 ttccgcctgt tcttgcttgc cgcgtgacat cagccagcga ggactttctg gcacggtata
     5881 cagcagcatt aagaacagca gtgcagggat acattccgag gcaaacatat aacgccagcc
     5941 gtcagtattc agccagctgg catcaccgga acgggcaata aaatagttta cgcagtaaac
     6001 taaaagttgc ccgaaaataa tcgcaaactg gttaaaagag accagtttcc cgcgaatatg
     6061 agctggagcc agttccgcaa tatacatggg cgagagcatt gaggctaaac caacgccaat
     6121 accgccaata atgcgataaa taacaaattc cgggacataa cctgccagat aaacaggcac
     6181 tgtgttgtcc gggtttatag aggtaaaacc aagttctggc caggcagaac ctacaccaga
     6241 aataaaaaac aggacagcag caatcttaag tgaatcacga cgaccgaagc ggctactgca
     6301 ataaccaccg agggcaccac cgatgatgca cccaatcaga gcgctggcta cgcaaaaccc
     6361 taacagggag ttggcagcgg attcacttaa gttttgtgga gcaacaaaga cggtattgag
     6421 tgactcaaca gtaccggaaa taacggcggt gtcgtagcca aataataaac cacctaatgt
     6481 agcgactaag gtaatcgaaa atatataact ggaattatac tgggtattca ttcagacctg
     6541 ccttagacca ttctgatctt ttatcagtgc attgatgttg gaattgtcag gcagttaagg
     6601 caattatcat tttttacaat gcgtttacgt a
//
LOCUS       DAZKFU020000246         6589 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZKFU020000246 DAZKFU020000000
VERSION     DAZKFU020000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.248"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..631
                     /locus_tag="ACHOQQ_RS20270"
                     /old_locus_tag="pgaptmp_004054"
     CDS             <1..631
                     /locus_tag="ACHOQQ_RS20270"
                     /old_locus_tag="pgaptmp_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472245.1"
                     /translation="VNHFRYKFLLSGNAGEMILYFANVFFNPLALSFIIATIICITIK
                     KRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGITVTVPSKQWVANIVKQ
                     GPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHVCDVQGFACAYQEGVQ
                     TMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKMMLSARQ"
     gene            754..1380
                     /locus_tag="ACHOQQ_RS20275"
                     /old_locus_tag="pgaptmp_004055"
     CDS             754..1380
                     /locus_tag="ACHOQQ_RS20275"
                     /old_locus_tag="pgaptmp_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472246.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1419..1664)
                     /gene="zapB"
                     /locus_tag="ACHOQQ_RS20280"
                     /old_locus_tag="pgaptmp_004056"
     CDS             complement(1419..1664)
                     /gene="zapB"
                     /locus_tag="ACHOQQ_RS20280"
                     /old_locus_tag="pgaptmp_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGU4472247.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            2090..2935
                     /gene="glpF"
                     /locus_tag="ACHOQQ_RS20285"
                     /old_locus_tag="pgaptmp_004057"
     CDS             2090..2935
                     /gene="glpF"
                     /locus_tag="ACHOQQ_RS20285"
                     /old_locus_tag="pgaptmp_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGU4472248.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2958..4466
                     /gene="glpK"
                     /locus_tag="ACHOQQ_RS20290"
                     /old_locus_tag="pgaptmp_004058"
     CDS             2958..4466
                     /gene="glpK"
                     /locus_tag="ACHOQQ_RS20290"
                     /old_locus_tag="pgaptmp_004058"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGU4472249.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4696..5706
                     /gene="glpX"
                     /locus_tag="ACHOQQ_RS20295"
                     /old_locus_tag="pgaptmp_004059"
     CDS             4696..5706
                     /gene="glpX"
                     /locus_tag="ACHOQQ_RS20295"
                     /old_locus_tag="pgaptmp_004059"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4472250.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5803..6549
                     /gene="fpr"
                     /locus_tag="ACHOQQ_RS20300"
                     /old_locus_tag="pgaptmp_004060"
     CDS             5803..6549
                     /gene="fpr"
                     /locus_tag="ACHOQQ_RS20300"
                     /old_locus_tag="pgaptmp_004060"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGU4472251.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKESSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
BASE COUNT         1615 a         1598 c         1712 g         1664 t
ORIGIN      
        1 cgttaaccat ttccgctaca aatttctttt atctggcaac gcgggtgaaa tgattttata
       61 ttttgctaac gtatttttta acccacttgc cttatcattt attatcgcca caatcatctg
      121 tataacgata aaaaaaagaa gcagccgcag ctttattcgc ggcacttgtt ggttaatggg
      181 attatttctt atctatagca gctatacggt gtgggaacga cacagcatat gggattatac
      241 tttcccggag ccaggtatca ccgtcacggt tccgagcaaa cagtgggttg ccaatatagt
      301 aaaacaagga ccaaccctgg tgacccggga tgcccatgct attttagcta ttgttgcctt
      361 ctcacaaaat gaactcggtg tgcattcaat cgaggaactt accgcgactc aaaacggaac
      421 tgcagaaatg catgtttgtg atgttcaggg atttgcctgc gcttatcagg aaggagtgca
      481 aacaatgagt gatggcaaag taagacatgc tattttcatg actctactgg ataacactaa
      541 tttgatacaa ctggtcgcag ctatcgaccc agattatttg gacgaatatc aagaagaagt
      601 aatgaaaatg atgctttctg ctcgtcaatg atctgatgtg catgttaata ataatggatt
      661 gatatgagat acgttatctt attaatacta tggataataa ctgtttttct tagtcatctt
      721 cagcacagta acttgcttac tggagatcct gggatggttg gcgagtatat cggttcagta
      781 cttcttgggc cgttattact tcccgtgctt gtgtcaggta ttctgtgcac attcactaaa
      841 aaaacgcgta acttcgccag ttttactcgc gggtgctgtt gggtattggg agtcctgctt
      901 ttatccaacg tcggcaatac gtttcgtctg ttcaccccgt ggcactatac gtttgagaag
      961 gcagctattt ccgtgactgt cccaaaccga cactggaata cggtttctat tagtactgat
     1021 aaaaccattg atataagaag tgaagataat agcgtcttta tttctgcttt ccgcctgccc
     1081 gcaggacgta gcgccgatga ctcgctggaa gagttaaaga agatgcagcg agacaatctc
     1141 aaggatcaat acaatgaaga aaccttccag tttcatgatt gtaatgccaa acatttcacc
     1201 tgtaagtatc aggatgtgtt gataaatttt gatggtcagc agaaaagaac catcagtgtt
     1261 tatcttgaag acacccccag ggctgtgggg atcattgcat taatggagcc agatacggca
     1321 gataaatatc gccaacaggc gatggagatt atgttgtctg ctaaaaatac agtgaagtaa
     1381 aaaataaggc gccttgcggc gcctctgcgt ggaagagatt aaacctcttc catgcgaccc
     1441 agcagggcct gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca
     1501 cgctccagct cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt
     1561 ttttctttca gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt
     1621 acttttgctt ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc
     1681 aaggcgttga tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga
     1741 gcgcacttta gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct
     1801 tgcagattac ggtttgccac acttttcatc cttctcctgg tgacataatc cacaccaatc
     1861 gaaaatgtta ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag
     1921 atggaataaa tggcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga
     1981 tatttctcgt ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg
     2041 tccgtgactt tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac
     2101 atcaaccttg aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt
     2161 cggtgtgggt tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat
     2221 cagtgtcatt tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg
     2281 cgcgcatctt aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg
     2341 caaagttatt ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt
     2401 ttacgggctt tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg
     2461 cagcgttgaa agtgttgatc tggctggcac tttctctact taccctaatc ctcatatcaa
     2521 ttttgtgcag gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct
     2581 ggcgttaacg gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg
     2641 tctactgatt gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc
     2701 agcgcgtgac ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt
     2761 taccggcggc agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc
     2821 gattgtaggt gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg
     2881 tgttgtggaa gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga
     2941 ctacgggaca attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca
     3001 ccagctcccg cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg
     3061 aatttgagca aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg
     3121 ccacccaaag ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa
     3181 ttgcagctat cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg
     3241 gcaagcctat ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc
     3301 atttaaaacg tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc
     3361 cgtacttttc tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc
     3421 gtgcgcgtcg tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga
     3481 ctcagggccg tgtccatgtg accgattata ccaacgcctc tcgtaccatg ttgttcaaca
     3541 tccataccct ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc
     3601 tgccagaagt gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg
     3661 gcacgcgtat tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt
     3721 tgtgcgtgaa agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga
     3781 acactggcga gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc
     3841 cgactggcga agtgaactat gcgttggaag gtgcagtgtt tatggcaggc gcatccattc
     3901 agtggctgcg cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca
     3961 ccaaagtgca aaacaccaat ggtgtgtatg tggttccggc atttaccggg ctgggtgcgc
     4021 catactggga cccgtatgcg cgcggggcga ttttcggctt aacgcgtgga gtgaacgcta
     4081 accacattat ccgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtactggaag
     4141 caatgcaggc cgactccggt attcgtctgc acgccctgcg cgtggatggc ggcgcagtag
     4201 caaacaattt cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg
     4261 aagttcgcga agtgactgca ttgggtgcgg cctatcttgc tggtctggcg gtcggtttct
     4321 ggcagaacct cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca
     4381 tcgaaaccac tgagcgtaat taccgttacg caggctggaa aaaagcggtc aaacgcgcga
     4441 tggcgtggga agaacacgac gagtaatgtc aggcacctga atcgattgcc tgatgctgat
     4501 cgcctgatgc gacgcttacg cgtcttatca ggcctacgag ccgatgccat tttgtgtagg
     4561 cgggataagg cgttcacgcc gcatccggca tcacgcagac tctgaaaccg tgcgggggca
     4621 accccgcaca catcaataat ccctcccttc ccctgtgcta cacttcgcgc cattccttac
     4681 tgcttagagt ttgctatgag acgagaactt gccatcgaat tttcccgcgt caccgaatca
     4741 gcggcgctgg ctggctacaa atggttagga cgcggcgata aaaacaccgc ggacggcgcg
     4801 gcggtaaacg ccatgcgtat tatgctcaac caggtcaaca ttgacggcac catcgtcatt
     4861 ggtgaaggtg aaatcgacga agcaccgatg ctctacattg gtgaaaaagt cggtactggt
     4921 cgcggcgacg cggtagatat tgctgttgat ccgattgaag gcacgcgcat gacggcgatg
     4981 ggtcaggcta acgcgctggc ggtgctggca gtaggcgata aaggctgttt cctcaacgcg
     5041 ccagatatgt atatggagaa gctgatcgtc ggaccgggag ccaaaggcac cattgatctg
     5101 aacctgccgc tggcggataa cctgcgcaat gttgcggcgg cgctcggcaa accgttgagc
     5161 gaactgacgg taacgattct ggctaaacca cgccacgatg ccgttatcgc tgaaatgcag
     5221 caactcggcg tacgcgtatt tgctattccg gatggcgacg ttgcggcctc aattctcacc
     5281 tgtatgccag acagcgaagt tgacgtgctg tacggtattg gtggcgcgcc ggaaggcgtg
     5341 gtttctgcgg cggtgatccg cgcattagat ggcgacatga acggtcgtct gctggcgcgt
     5401 catgacgtca aaggcgacaa cgaagagaat cgtcgcattg gcgagcagga gctggcacgc
     5461 tgcaaagcga tgggcatcga agccggtaaa gtattgcgtc tgggcgatat ggcgcgcagc
     5521 gataacgtca tcttctctgc caccggtatt accaaaggcg atctgctgga aggcattagc
     5581 cgcaaaggca atatcgcgac taccgaaacg ctgctgatcc gcggcaagtc acgcaccatt
     5641 cgccgcattc agtccatcca ctatctggat cgcaaagacc cggaaatgca ggtgcacatc
     5701 ctctgattga tttgatcgat tgagccttcc agtccttcgg gactggaatt tttttgttcg
     5761 gagaacgaag ataaggcaag tcaatcaaaa caggagaaaa acatggctga ttgggtaaca
     5821 ggcaaagtca ctaaagtgca gaactggacc gacgccctgt ttagtctcac cgttcacgct
     5881 cccgtgcttc cgtttaccgc cgggcaattt accaagcttg gccttgaaat cgacggcgaa
     5941 cgcgtccagc gcgcctactc ctatgttaac tcgcccgata atcccgatct ggagttttac
     6001 ctggtcaccg tccccgatgg caaattaagc ccacgattgg cggcactgaa accgggcgat
     6061 gaagtgcagg tggttagcga agcagctggc ttctttgttc tggatgaagt accagattgc
     6121 gaaacgctat ggatgctggc aaccggtaca gcgattggcc cttatttatc gattctacaa
     6181 ctaggcaaag atttagatcg cttcaaaaat ctggtcctgg tgcacgccgc acgttatgcc
     6241 gccgacttaa gctatttgcc actgatgcaa gaactggaaa aacgctacga aggaaaactg
     6301 cgcattcaga cggtggtcag tcgggaaacg gcagcgggtt cgctcaccgg acggataccg
     6361 gcgttaattg aaagtggtga actggaaagc gcggttggcc tgccgatgaa taaagaaagc
     6421 agccatgtga tgctgtgcgg caatccacag atggtgcgcg atacgcaaca gttgctgaaa
     6481 gagacccggc agatgacgaa acatttacgt cgccgaccgg gtcacatgac agcggagcat
     6541 tactggtaag cggttactta tcgataaacg gcacgatcaa gagatccgc
//
LOCUS       DAZKFU020000247         6584 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZKFU020000247 DAZKFU020000000
VERSION     DAZKFU020000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.249"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..274)
                     /gene="yqfB"
                     /locus_tag="ACHOQQ_RS20305"
                     /old_locus_tag="pgaptmp_004061"
     CDS             complement(<1..274)
                     /gene="yqfB"
                     /locus_tag="ACHOQQ_RS20305"
                     /old_locus_tag="pgaptmp_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGU4472252.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPG"
     gene            319..1752
                     /gene="bglA"
                     /locus_tag="ACHOQQ_RS20310"
                     /old_locus_tag="pgaptmp_004062"
     CDS             319..1752
                     /gene="bglA"
                     /locus_tag="ACHOQQ_RS20310"
                     /old_locus_tag="pgaptmp_004062"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGU4472253.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDILREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            complement(1918..4791)
                     /gene="gcvP"
                     /locus_tag="ACHOQQ_RS20315"
                     /old_locus_tag="pgaptmp_004063"
     CDS             complement(1918..4791)
                     /gene="gcvP"
                     /locus_tag="ACHOQQ_RS20315"
                     /old_locus_tag="pgaptmp_004063"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGU4472254.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLADLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAGWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4909..5298)
                     /gene="gcvH"
                     /locus_tag="ACHOQQ_RS20320"
                     /old_locus_tag="pgaptmp_004064"
     CDS             complement(4909..5298)
                     /gene="gcvH"
                     /locus_tag="ACHOQQ_RS20320"
                     /old_locus_tag="pgaptmp_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGU4472255.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDVLSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(5322..6416)
                     /gene="gcvT"
                     /locus_tag="ACHOQQ_RS20325"
                     /old_locus_tag="pgaptmp_004065"
     CDS             complement(5322..6416)
                     /gene="gcvT"
                     /locus_tag="ACHOQQ_RS20325"
                     /old_locus_tag="pgaptmp_004065"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGU4472256.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVEKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
BASE COUNT         1526 a         1783 c         1722 g         1553 t
ORIGIN      
        1 gaccgggata gatgtcggca atgacctttt tcagttcagt cagggtcata ttttcctgct
       61 ctgcatgttt ttccgtcagc gtatccagcg ttacggttga ggttgcggtg acttcaatcg
      121 tgcaaaaata accgtcatct tcaaaacgcc cgacacgaag cacatcaccc gttttgaagt
      181 gcgattcaga ctcgtcgcgg atggtgatgg ttttacgccc agccagaatg tcatcctgga
      241 aacgttgaaa aaaagtgatg tcgtttggct gcatggtact atttcctgta agaattgact
      301 catctggagc ctatgatagt gaaaaaactc accttaccga aagatttctt atggggcggc
      361 gcagttgccg ctcatcaggt cgaaggcggc tggaacaaag gcggcaaagg gccgagcatt
      421 tgtgacgttc tgaccggtgg cgcacacggc gtgccgcgcg aaatcaccaa agaagtcttg
      481 ccaggaaaat actatccaaa ccatgaagcc gttgattttt atggtcacta caaggaagac
      541 atcaagctat ttgccgaaat gggcttcaaa tgttttcgta catccatcgc ctggacgcgc
      601 atttttccaa aaggcgatga agctcagcca aacgaagaag ggctgaagtt ttacgactct
      661 ctgttcgatg aattgctgaa atacaacatc gaaccagtga tcaccctctc ccactttgaa
      721 atgccgctgc atctggtgca gcaatacggt agctggacca accgtaaagt ggttgatttc
      781 tttgtccgtt tcgcggaagt ggtatttgaa cgctataagc ataaagtcaa atactggatg
      841 accttcaacg aaattaacaa ccagcgtaac tggcgtgcac cgctgttcgg ttactgctgc
      901 tccggcgtgg tttataccga gcatgagaac ccggaagaga cgatgtatca ggtgctgcat
      961 caccagtttg tcgccagcgc cctggcggtg aaagctgcgc gtcgcattaa cccggagatg
     1021 aaagtcggct gtatgctggc gatggtgccg ctctatcctt actcctgtaa cccggacgat
     1081 gtgatgttcg ctcaggagtc gatgcgcgaa cgctacgtct ttaccgatgt gcagctacgc
     1141 ggctattacc cgtcctatgt gttgaacgag tgggagcgtc gcggatttaa catcaaaatg
     1201 gaagatggcg atctcgatat actgcgcgaa ggcacctgcg attatcttgg tttcagctat
     1261 tacatgacca acgcagtgaa ggccgaaggc ggcaccggcg atgcgatctc tggtttcgaa
     1321 ggcagcgtac cgaacccgta tgttaaagcg tctgactggg gctggcagat tgatccagta
     1381 ggtctgcgct atgccctttg cgaactgtat gagcgttatc agaggccgct gtttattgtc
     1441 gaaaacggtt ttggcgctta cgacaaagtg gaagaagatg gcagcatcaa cgatgactac
     1501 cgcattgact acctgcgcgc ccatatcgaa gagatgaaaa aagcggtgac ttacgatggc
     1561 gtggatctga tgggctacac accgtggggc tgcatcgact gcgtgtcgtt caccaccggg
     1621 cagtacagca aacgctacgg ctttatctat gtgaataaac atgacgacgg tactggcgat
     1681 atgtcgcgtt cacgtaagaa gagctttaac tggtacaaag aggtgattgc cagcaacggc
     1741 gagaatctgt aattagccga tgtgcctgat gcgatacgct catcaggcct ggcactcttt
     1801 taatatattg agctcgcaag attttatatt acgcctcagc cgaaacacac aaagcgcact
     1861 ttattaacaa gctaaaaagg cgccgaagcg cctttagaag agagtcgaat cagtgaatta
     1921 ctggtattcg ctaatcggta cgcaggaaca gaacaggtta cggtcgccgt aaacatcatc
     1981 cagacgtttc accgtcggcc agtatttgtc tgccacgcca gccgggaata ccgcaacttc
     2041 acggctgtac ggatgcgccc acccggcgac cagttcgctc tgaatgtgcg gcgcgttcac
     2101 caacgggtta tcttccagcg gccagacacc ggctttcacc tggtcaattt ctgcgcggat
     2161 agccagcatc gcatcgataa agcgatccag ttccactttg ctttcagatt cagttggttc
     2221 aaccatcagc gtacccgcca ccgggaacga catggtcggc gcgtggaaac cgtagtcgat
     2281 caggcgcttg gcaatatcca gctcgctgat gccggtttct tctttcagcg gacgaatatc
     2341 gagaatacat tcgtgcgcca cgcgaccgtc acgaccggta tacagcaccg ggaaggcatc
     2401 ctgcaggcgg ctggcaatat agttggcgtt gaggattgcc acctgacttg cttttttcag
     2461 cccttctgcg cccatcatgc ggatgtacat ccagctgatt ggcaggatag aggcgctacc
     2521 gaacggtgcc gcagaaaccg cgccctgacg ggttaacatg ccttcgattt gcaccacgct
     2581 atgacccggt acaaacggag ccagatgcgc tttcacaccg atcggtccca tacccggacc
     2641 accaccgccg tgcggaatgc agaaagtttt atgcaggttg aggtgcgaaa cgtccgcgcc
     2701 aatgaagcct ggcgaggtga tgccaacctg ggcgttcatg ttcgcgccat caaggtaaac
     2761 ctgaccgccg aactgatgca cgacttcaca cacttcacgg atcgtttctt catacacacc
     2821 gtgggtggaa ggataggtca ccatgataca ggagaggtta tcgcccgcct gttccgcttt
     2881 cgcgcgcaga tcagccagat cgatgttgcc gtttttatca catgccacaa ccaccacctg
     2941 cattcctgcc atatgtgcag aagcagggtt agtgccgtgc gcagaagccg ggatcaggca
     3001 gatatcgcga tgcccttcgt tgcggctttc atgataatga cgaatcgcca gcaggcccgc
     3061 gtattcgccc tgtgcgccag agttcggctg catacagacg gcgtcgtaac cggtcagttt
     3121 caccagccag tcagccagct gcgcgatcat ctgctgataa ccttcggcct gttccggcgg
     3181 gcagaaaggg tgcagttcgg caaattccgg ccaggtgatg gggatcatct cggcggcggc
     3241 gttcagtttc atggtgcagg aacccagcgg gatcatcgcc tgattcagcg ccagatcttt
     3301 acgctccagc gagtgcatat agcgcatcat ttcggtttcg ctgtggtagc gattaaacac
     3361 cggatgggtg aggatttcgt catcacgcag catcgcaggt tgaatagagc ggctgtcgtg
     3421 agccacgtct ttgtccagcg tgtcgatgtc cagcccgtga ttatccccca gtagtacgct
     3481 gaaaagctgc atcacgtttt cgcgcgtggt ggtttcatca agggtgatcc caaccgcgtt
     3541 caggatatcg ctacgcaggt tgatttcagc cgcttcggca cgcgccagta cgcccgcctt
     3601 gtcggccact tccacacaca gagtgtcgaa atagtgcgca tggcgcagct tcagaccttt
     3661 ttgttgcagg cctgccgcca ggatatcggt cagacggtga atgcggttag cgatacgttt
     3721 caggccaatc gggccgtgat aaacggcata caggctggcg atgtttgcca gcagcacctg
     3781 ggaagtacaa atgttggagt tcgctttctc acgacgaata tgttgctcgc gagtctgcat
     3841 cgccatgcgc agcgcggtat tgccagctgc atctttcgat acaccgataa tacggcctgg
     3901 cattgagcgt ttgtattcat ctttcgccgc aaagaatgcc gcgtgtgggc caccgtagcc
     3961 catcggcacg ccgaagcgtt gcgccgaacc aaagacaata tctgcgccct gtttacccgg
     4021 cgcagttaac agcaccagcg ccataatatc ggcggcgacg ctgaccacaa ttttgcgtga
     4081 tttcagttcg ctgataagcg cagtgtagtc gtgaatttca ccagtagtac cgacctgctg
     4141 taacagcacg ccgaagacgt cctgatggtc gagcactttt tgcgcatcat cgacaatcac
     4201 ttcaaaacca aaggtttcag cacgagtacg taccacatcc agcgtttgcg gatgaacgtc
     4261 agaagcaaca aagaagcggt tggcattttt cagtttgctg acgcgtttcg ccatcgccat
     4321 tgcttcggcg gcagcggtgg cttcgtcaag aagagaagca gaggccatat ccagcccggt
     4381 taaatccagc gttacctgct ggaaattgag cagtgcctca aggcggccct gcgagacttc
     4441 aggttgatac ggagtatacg cggtatacca gcccggattt tccagcatgt tacgcaggat
     4501 aaccggcggt aactgcacgg cggtgtaacc catgccgatg taagacgtga agcgtttatt
     4561 gcgactggca atagccttga gttctgccag tgcagcgtat tcggtcgccg gtgcgccaac
     4621 ctgcggcggg gtcgcaagct gaatatcttt cggtacaatc tggccggtca gcgcgtttaa
     4681 cgattgtgcg ccaacggcat tcagcatttc ttgctgttgc gcggcgtccg gtccgatatg
     4741 gcgttcaata aaagcgccgc tattttcaag ctggcttaac gtctgtgtca tgagcgatgg
     4801 ttcctgaaac gtgcagtgaa ttgtgaacct ctctccttac gaagagagtg agggtgaggc
     4861 gtaaatttcc tcaccctgat cctctcccgc agaagaggaa taaagccgtt actcgtcttc
     4921 taacaatgct tcgtatgcgg tcgcatccag cagtgattcc agttcgcttt catcgctggc
     4981 tttgattttg aaaatccagc cgcctgcata cggttcgctg ttcaccagtt ccggggaatc
     5041 gctcagtacg tcgtttaccg ccacgatttc accgcttact ggtgcataaa tgtctgacgc
     5101 cgcttttacc gattcggcaa ccgcgcagtc atcgcccgcg ctaaccgtcg cgcccacttc
     5161 cggcaggtca acaaacacca tatcgcctaa cagctcctga gcgtgttcgg tgatgccaac
     5221 ggtgtaagtg ccgtcggctt ctttacgcag ccattcgtgt tctttgctgt atttcagttc
     5281 tgctggtacg ttgctcatca atcaatctcc aaaaaagtaa atcacgcgac ggctttgccg
     5341 ttacgcacaa aaacaggttt cgtcacttta accggcattt cacggttgcg aatttgcaca
     5401 atcgctgttt caccaatacc ttccggcacg cgcgccagcg caatgctgta acccagcgtc
     5461 ggggagaaag tcccgctggt gataatgcct tcatgctggt tgccctgcgc atcggtaaaa
     5521 cgtaccggca gttcattacg cagcacgcct ttttcggtca tcaccagacc aaccagtttt
     5581 tctgtaccat gctcacgctg cacttccagg gcttcacgac cgatgaagtc acgatctgcc
     5641 ggttcccagg cgatagtcca gcccatattg gcggctaaag gagagatggt ttcgtccatc
     5701 tcctgactat aaagattcat acccgcttcc agacgcagcg tgtcacgcgc gcccaggccg
     5761 catggcttaa cgcccgcttc caccagcgcg cgccagaaat ccgccgcttt ttcattgggc
     5821 agcgcaattt cgtagcccgc ttcaccggta taaccagtgg tggcaataaa cagatcgccc
     5881 gcctgcacgc caaagaacgg tttcatccct tccaccgcct gacgctgggc gtcattaaac
     5941 agtgtggcag cttttgcctg cgcattcggc ccttgcacgg caatcatgga aaggtcatcg
     6001 cgaacggtaa tttcgatgcc gaaaggttca gcgtgttggg taatccagga gaggtctttt
     6061 tcgcgggtgg cggagttaac aacgaggcgg aagaaatctt cagtaaagta gtagacgatg
     6121 agatcatcta tcacaccgcc agaggcattc aacatccccg agtaaagggc tttgccgctt
     6181 ttggtgagct tctccacatc gttcgccagc agataacgca gaaactcccg ggtgcggctg
     6241 ccgcggagat cgacgatggt catatgcgac acatcaaaca ttccggcatc ggtacgtacc
     6301 gcatgatgtt cgtcgatttg cgaaccgtaa tgcagcggca tcatccagcc gtggaaatcc
     6361 accatgcgag cgccgcaaag cgtgtgttgt tcgtacaaag gagtctgttg tgccatcttg
     6421 tcctcattga ataagcgggg ctgacaactt tttcatggtg aaattatcac cacaaaaccc
     6481 agcatcggag ccactcccgg tccccaacgc aatcgttctc ttttgcctga acttaccacc
     6541 gaaacagact gttaaccata aggtaaaatt gatcatcaca ttag
//
LOCUS       DAZKFU020000248         6558 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZKFU020000248 DAZKFU020000000
VERSION     DAZKFU020000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.250"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..177
                     /locus_tag="ACHOQQ_RS20330"
                     /old_locus_tag="pgaptmp_004066"
                     /pseudo
     CDS             1..177
                     /locus_tag="ACHOQQ_RS20330"
                     /old_locus_tag="pgaptmp_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(266..1402)
                     /gene="yibH"
                     /locus_tag="ACHOQQ_RS20335"
                     /old_locus_tag="pgaptmp_004067"
     CDS             complement(266..1402)
                     /gene="yibH"
                     /locus_tag="ACHOQQ_RS20335"
                     /old_locus_tag="pgaptmp_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4472257.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1405..1767)
                     /gene="yibI"
                     /locus_tag="ACHOQQ_RS20340"
                     /old_locus_tag="pgaptmp_004068"
     CDS             complement(1405..1767)
                     /gene="yibI"
                     /locus_tag="ACHOQQ_RS20340"
                     /old_locus_tag="pgaptmp_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4472258.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2304..4217
                     /gene="mtlA"
                     /locus_tag="ACHOQQ_RS20345"
                     /old_locus_tag="pgaptmp_004069"
     CDS             2304..4217
                     /gene="mtlA"
                     /locus_tag="ACHOQQ_RS20345"
                     /old_locus_tag="pgaptmp_004069"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGU4472259.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMFLAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGHK"
     gene            4447..5595
                     /gene="mtlD"
                     /locus_tag="ACHOQQ_RS20350"
                     /old_locus_tag="pgaptmp_004070"
     CDS             4447..5595
                     /gene="mtlD"
                     /locus_tag="ACHOQQ_RS20350"
                     /old_locus_tag="pgaptmp_004070"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4472260.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDANAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5595..6182
                     /gene="mtlR"
                     /locus_tag="ACHOQQ_RS20355"
                     /old_locus_tag="pgaptmp_004071"
     CDS             5595..6182
                     /gene="mtlR"
                     /locus_tag="ACHOQQ_RS20355"
                     /old_locus_tag="pgaptmp_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGU4472261.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPHFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6194..6403)
                     /gene="yibT"
                     /locus_tag="ACHOQQ_RS20360"
                     /old_locus_tag="pgaptmp_004072"
     CDS             complement(6194..6403)
                     /gene="yibT"
                     /locus_tag="ACHOQQ_RS20360"
                     /old_locus_tag="pgaptmp_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGU4472262.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         1629 a         1656 c         1677 g         1596 t
ORIGIN      
        1 atcgcaatag atggaaaagc actcctgctc ttttttgatt agagccgtcg caggaggaac
       61 aactcatatc atcattgcga tctcgacaat gtatagtctg atccttcggc agatcaagac
      121 ggataaaaaa tccaatgagt tcacactcat cccgaacttc ttaacataat ggatattaac
      181 gaaaaatcat cacagccgat gaaataatat gcaaaaataa ttagtcctta cagggagcaa
      241 agttccataa tctccccctt ccccctcaat gatccaaata aagataatgc atccagctgg
      301 tcattcttag cagcactttt cgcatcaccg aaacatggct gaaatggtcg gagtaaactg
      361 ccacctgggc gtaaatgccg tcgggtaagg catcgatatc atcgttaggg tccagttcaa
      421 tggttcccag cacaccgtcc gtgccgggca cgaccgttaa tgattgcaat accccctgcg
      481 cctgataaga accgcctggc acgacaggta aaatactggt cagtttgccg tggaacacct
      541 gcccaggtag cgcgttaaac accacttccg catcatcgcc aggtttcaga cgtaacagcg
      601 agttttgccg aaattgggcg acaatttgcc gtttttgctc ggggatgaaa accatcaccg
      661 gacgcaacgg caaggcagct gcgtatgtgc ctgggcggat cagtacctga gtgacgtagc
      721 cattgcttgg tgcgcgaatg acagtctgct caagattata ttttgcttca gtaagttgcg
      781 ctcttaagct cacaatctgc gattgctcgc cgttgaccat actgtcgagc tgactctgga
      841 tctgcgcctg ctccgccacc gagcctttca ctaacgcatc ctgcgcgagg aaattttgcc
      901 gcgcatcgtc gatgtcacgt tccgagaacg gattcaccgc cgcctggctg cctttcaggt
      961 aacgttgata atttttaaac agacggtcgc gctccgctga aacctgggtg gtgttggcct
     1021 gcgcttcagt gagctgggcg cgcagcgttt ttatattatg cgtcgccgtc atcaggtcag
     1081 cctgaagtct gtcaactcgc gcctggtaac gaaccgggtc gagcttaaaa agcacctcgc
     1141 ccttttgaat aagctgatta ttcttgtcag tgacttcagt aacaattccc gtcacctgtg
     1201 gcgtgatagg gatcgctatc actgcctttt gcgcggtaaa agtgtaaggg tggttgtagt
     1261 tcatcaacaa aatcaaacca ctcaccagaa acacgcctcc cagcgccgcc gtcgccagcg
     1321 tccactgatt caccggaata cgaaagattt taaagaccgc ccacgccagc gccacgtaag
     1381 ttaaaacaat caatagatcc ataattagat ctccgggaac gtggaatagt cagtcttttt
     1441 ctcggcggcg agttgatgct ccagccggga aattcggtca gaaagtgcgg cgatttccgg
     1501 gtcagtcgct ttctcctgcg acgcaacatg tgactgcata ccccagccac gctccggttg
     1561 atagagcgtc gcccagatcc acagaaacgg ccagataaca tgcagagtaa acaggctcac
     1621 ccagcccgcc gtatgaatag cgtcggcatg gggatggttg cgctttttgg caatcagata
     1681 agggatgtca tgtatcgcga tgattccata aaaaatcacc agaaagacga agatcagcac
     1741 tcccaacgcg aaatagttta gaaacatatc tgcctcggat tcacgtttat cagtgttgtt
     1801 ttttggctgg cagccagaag ggagtcaggc tgatattttg acaataatcc gggttcgcga
     1861 ttctcgccat aacaccaaag aataattttt agaggtgatg agttgcttag ttacataacg
     1921 attgtatgac gaaggcataa catgctgtag atcacatcag gtgaacgccg taagaaaata
     1981 tcttgtgatt cagatcacaa agattcaaca aaccatcaaa acaaaaatgt gacattactc
     2041 acatttaaat gccattttta gcgaaaatcg cctccttgtt gcttttttac acaagcgttt
     2101 tgtgatgaac gtcacgtcaa ttacctctct accccctata tttatgtgat tgatatcaca
     2161 caaaaggccg tcgactggac agttaaccga ttcagtgcca gatttcgcag tatctacaag
     2221 gtccggctac ctctgccgcc acattaacaa aaaacctcgg gcttccagcc tgcgcgacag
     2281 caaacataag aaggggtgtt tttatgtcat ccgatattaa gatcaaagtg caaagctttg
     2341 gtcgtttcct cagcaacatg gtgatgccaa atatcggcgc gtttatcgcg tggggtatca
     2401 tcaccgcgtt atttattcca acagggtggt taccgaacga gacgctggcg aagctggtcg
     2461 ggccgatgat cacttatctc ctgccgctgc tgatcggtta taccggtggt aagctggtag
     2521 gcggcgaacg tggcggcgta gtcggtgcca tcaccaccat gggcgttatc gtcggcgcag
     2581 acatgccgat gttcctcggt tctatgattg caggtccgct gggcggctgg tgcattaagc
     2641 acttcgaccg ctgggtagac ggtaagatca aatccggttt tgagatgctg gtgaataact
     2701 tctccgcagg catcatcggg atgttcctcg ccattctggc atttctcggc attggcccga
     2761 ttgttgaagc cctgtccaaa atgctggctg cgggcgttaa cttcatggtt gtccatgaca
     2821 tgctgccgct ggcgtctatc tttgttgaac cggcgaaaat cctgttcctc aacaacgcca
     2881 ttaaccacgg tatcttctcg ccgctgggta ttcagcagtc ccatgaactg ggtaaatcca
     2941 tattcttcct gattgaagct aacccaggtc cgggtatggg cgtgctgctg gcgtacatgt
     3001 tctttggtcg tggtagcgct aaacagtctg cgggcggtgc ggcaattatc cacttcctgg
     3061 gtggtatcca cgaaatttac ttcccgtatg tgttgatgaa tccgcgtctg atcctggccg
     3121 ttatcctcgg cggtatgact ggcgtgttca cgctgactat cctgggcggt ggtctggttt
     3181 ctccggcatc tccgggttct atccttgctg tactggcgat gacaccaaaa ggtgcttatt
     3241 tcgctaacat cgcgggtgtg tgtgcggcga tggctgtctc ctttgttgtc tctgctattt
     3301 tgctgaaaac cagcaaagtg aaagaagaag atgatattga agcagcaact cgtcgtatgc
     3361 aggacatgaa agctgagtct aaaggtgcat ctccgctgtc tgcgggcgat gtgactaacg
     3421 acctgagcca cgtacgtaaa atcatcgttg cctgtgacgc cggtatgggt tcaagtgcga
     3481 tgggcgcagg cgtgctgcgt aagaaaattc aggatgcagg tctgtcgcag atttcggtca
     3541 ctaacagtgc gatcaacaac ctgccgccag atgtggacct cgtcatcact caccgtgacc
     3601 tgaccgaacg cgctatgcgc caggttccgc aggcacagca tatttcgctg accaacttcc
     3661 tcgacagcgg cctgtacacc agcctgaccg aacgtctggt tgctgcccag cgccatactg
     3721 aaaacgaagt gaaagtgaaa gacagtctga aagacagctt tgacgattcc agtgctaacc
     3781 tgttcaaact gggcgcggag aacatcttcc tcggtcgcaa agcggccacc aaagaagaag
     3841 cgattcgttt tgctggcgag cagctggtga aaggcggtta cgttgagccg gaatacgttc
     3901 aggcgatgct ggatcgtgaa aaactgaccc cgacttatct gggtgagtct atcgcggtgc
     3961 cacacggtac ggttgaagcg aaagatcgcg tactgaaaac gggcgtggtg ttctgccagt
     4021 acccggaagg cgtgcgcttc ggtgaagaag aagatgacat tgcccgtctg gtgattggta
     4081 ttgctgcccg taacaacgag cacattcagg ttatcaccag cctgaccaat gcactggatg
     4141 atgagtccgt catcgagcgt ctggcacaca ccaccagcgt ggatgaagtg ctggaactgc
     4201 tggcaggtca taagtaatcc aatcccaccc tctccacatg gagaaggtgg ggctaattgc
     4261 ctgatgcgct acgcttatca ggcctacagg atgcatcaca atttgttgaa tttgcacgtt
     4321 cttgtaggcc ggataaggcg tttacgccgc atccggcgcg gcccctctcc acatggagag
     4381 ggtttgggtg agggaaaagc ctcaccccag ccctctcggg taaaaacatt gatgaaggtt
     4441 aatactatga aagcattaca ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa
     4501 ctgctggcag acgcgggtat acaactgacg tttgccgatg tcaatcaggt ggtacttgat
     4561 gccctgaatg cccgtcatag ctatcaggtt catgtggtag gtgaaaccga gcaggtagat
     4621 accgtttccg gcgtcaatgc tgtcagcagc attggtgatg atgtcgttga tctcattgct
     4681 caggttgatt tagtcactac cgccgttggc ccggtagtgc tggaacgtat tgctccggct
     4741 atcgccaaag ggctggtgaa acgtaaagaa caaggtaatg aatccccgct gaacatcatc
     4801 gcctgtgaaa acatggtacg cggtaccacg cagctgaaag gccatgtgat gaacgccctg
     4861 ccggaagacg ccaacgcgtg ggtagaagaa cacgttggct ttgtcgattc cgccgttgac
     4921 cgcatcgtac cgccttcggc ttcggcaact aacgatccgc tggaagtgac ggtagaaacc
     4981 ttcagcgaat ggattgtcga taaaacgcag ttcaaaggtg cactgccgaa catcccaggc
     5041 atggagttaa ccgacaacct gatggcattt gtcgaacgta aactcttcac cctgaacacg
     5101 ggtcatgcta taaccgcgta cctcggaaaa ctggccggtc atcagaccat tcgtgacgct
     5161 attctcgacg agaaaatccg cgcggtggta aaaggtgcga tggaagaaag tggcgcggta
     5221 ctgatcaagc gttacggctt tgacgctgac aagcatgcgg cgtacatcca gaaaatcctc
     5281 ggccgttttg agaacccgta tctgaaagat gatgtagagc gcgtaggccg tcagccgctg
     5341 cgtaaactga gcgcaggcga ccgtctgatc aagccactgc tcggtacgct ggaatatggt
     5401 ctgccacata aaaacctgat tgaaggtatt gccgctgcaa tgcacttccg cagtgaagat
     5461 gatccgcagg ctcaggaact ggcagcactg atcgctgaca aaggtccgca ggcggcgctg
     5521 gcacagattt ccggtcttga tgccaacagc gaggttgtat ctgaggcggt aaccgcttat
     5581 aaagcaatgc aataatggtg gaccaggcgc aggacaccct gcgcccgaat aacagattgt
     5641 cagatatgca ggcaacaatg gaacaaaccc aggcctttga aaaccgtgtg cttgagcgtc
     5701 tgaatgctgg caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg
     5761 cggtaaatct tctggtgctt caggtattcc gcaaagacga ttacgcggtg aagtatgctg
     5821 tagaaccgtt actcgacggc gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca
     5881 tttacgggtt gggcgtcatt aaccgccagg aatacgaaga tgcggaactg ctgatggcat
     5941 tgcgtgaaga gctaaatcac gacggcaacg agtacgcgtt taccgacgac gaaatccttg
     6001 gaccctttgg tgaactgcac tgcgtggcgg cgttaccacc gccgccacat tttgaaccag
     6061 cagactccag tttgtatgca atgcaaattc agcgctatca acaggctgtg cgatcaacaa
     6121 tggtcctttc actgacagag ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt
     6181 aagcaacgtc tgcttactgc ccctctacct gcttcggccg ataaagccga cgataatact
     6241 ccagacgttg tagatataac ggcacgcttt catcgggtat tccggacgga atcgcgttac
     6301 ggggaggaag ttttttcaga tactcccgga acgcctggct tgatgccatg aaatctacgg
     6361 ctttatcgat aagaagcgga acgttttcac ctaatttgcc catgatgata tctccgtatt
     6421 accccgcgcc gggaatgcgc ggccgccaat tttagtttag gttcccggta aagatgacat
     6481 taaggaaaac gtgctgaatc ctcaataatc atccgcttct gtttattatc ttttttctat
     6541 agatttctta attaatca
//
LOCUS       DAZKFU020000249         6553 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZKFU020000249 DAZKFU020000000
VERSION     DAZKFU020000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.251"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(67..1434)
                     /gene="ydfJ"
                     /locus_tag="ACHOQQ_RS20365"
                     /old_locus_tag="pgaptmp_004073"
     CDS             complement(67..1434)
                     /gene="ydfJ"
                     /locus_tag="ACHOQQ_RS20365"
                     /old_locus_tag="pgaptmp_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HGU4472263.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            complement(1492..2511)
                     /gene="rspB"
                     /locus_tag="ACHOQQ_RS20370"
                     /old_locus_tag="pgaptmp_004074"
     CDS             complement(1492..2511)
                     /gene="rspB"
                     /locus_tag="ACHOQQ_RS20370"
                     /old_locus_tag="pgaptmp_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HGU4472264.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHVDGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWVINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(2523..3737)
                     /gene="rspA"
                     /locus_tag="ACHOQQ_RS20375"
                     /old_locus_tag="pgaptmp_004075"
     CDS             complement(2523..3737)
                     /gene="rspA"
                     /locus_tag="ACHOQQ_RS20375"
                     /old_locus_tag="pgaptmp_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HGU4472265.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTITPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(3943..4269)
                     /gene="ynfA"
                     /locus_tag="ACHOQQ_RS20380"
                     /old_locus_tag="pgaptmp_004076"
     CDS             complement(3943..4269)
                     /gene="ynfA"
                     /locus_tag="ACHOQQ_RS20380"
                     /old_locus_tag="pgaptmp_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGU4472266.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4404..4745
                     /gene="ynfB"
                     /locus_tag="ACHOQQ_RS20385"
                     /old_locus_tag="pgaptmp_004077"
     CDS             4404..4745
                     /gene="ynfB"
                     /locus_tag="ACHOQQ_RS20385"
                     /old_locus_tag="pgaptmp_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGU4472267.1"
                     /translation="MKITLSKRIGLLAFLLPCTLALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            4780..5340
                     /gene="speG"
                     /locus_tag="ACHOQQ_RS20390"
                     /old_locus_tag="pgaptmp_004078"
     CDS             4780..5340
                     /gene="speG"
                     /locus_tag="ACHOQQ_RS20390"
                     /old_locus_tag="pgaptmp_004078"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HGU4472268.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5343..6053)
                     /gene="ynfC"
                     /locus_tag="ACHOQQ_RS20395"
                     /old_locus_tag="pgaptmp_004079"
     CDS             complement(5343..6053)
                     /gene="ynfC"
                     /locus_tag="ACHOQQ_RS20395"
                     /old_locus_tag="pgaptmp_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGU4472269.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6161..6466
                     /gene="ynfD"
                     /locus_tag="ACHOQQ_RS20400"
                     /old_locus_tag="pgaptmp_004080"
     CDS             6161..6466
                     /gene="ynfD"
                     /locus_tag="ACHOQQ_RS20400"
                     /old_locus_tag="pgaptmp_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGU4472270.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1831 a         1651 c         1409 g         1662 t
ORIGIN      
        1 ggcaagctac atgttttgtt atatgaataa aaaatcccct ctccggtaag agaagggatt
       61 gaggatttaa agacttctgg aaggttgcgc agctcttaca acacgcggtt gatcttccgc
      121 agcgtcttcc agcgcactta aatcacggtc tttcacttct ggcattttca gcgcagagat
      181 taaaccaatc actgaatatg ccatgatcat aatggcgatc ggataccagg attccgtcat
      241 ggtgcagaaa atacccgcaa ggataggacc aaaaccggaa gcgataagac caccaatttc
      301 tttagaaata gccatccggg taaagcggtt tttacagccg aacatttctg ccatggtaat
      361 gttttccaga gcaaataatc ccagcaccgc acagttatga atcacaatca gtgcaaccat
      421 aatggtgctc ggggcatagc ttttatctac gatgatagaa agcattggcc atgccagcac
      481 aatcgcagag gtattcataa taatatacgg gatccggcga ccaattttat cggataacca
      541 accaaggaac ggaatggtca taaagccgag aatcgaactg atcatcaatg catctgttgg
      601 aattgctttg ttaaacaata acgtctgtac taaatagcct gcaaggaaag tctgaattaa
      661 cccggagtta cctgcctgac caaaacgcag ccctgttgcc agccagaagg atttgctctg
      721 gaacatgcta ccagcaggtg caggttttgc tgtcggttgg ttgctgtcgt taaccttctc
      781 aaagaccggg ctttctttca gattcatacg caaccagata gcaaagacca tcacgacaac
      841 gctcgccagg aacggtatac gccatcccca cgccagcagt tcctctttac tgagaatgaa
      901 gaacataaag gcccagattg ccgttgcgct caaggttccg cagttagttc ccatagccac
      961 aaatgaggag ataattccgc gcttaccttt tggcgcatat tccgccagca tcgtaccggc
     1021 accggaaatt tccgcacctg cacccaatcc ctgaataata cgcagcgtca ccagcaagat
     1081 gggggcaaaa acaccaatct gtgcataggt cggtaacact ccaattaagg tggtacagat
     1141 ccccatcatg gtgatggtaa taaagagcac ttttttacgc cctattctgt cgcccatttt
     1201 gccgaaaata aatgctccga caatacgcgc cacataacct gcaccgtagg ttcccattgc
     1261 cagaattaac gccattgccg ttgatgattc aggaaaaaat atttcatgaa acactaacgc
     1321 tgcgccgagc gaatataact ggaaatccat aaattcaagc gcagtgccca gccatcccga
     1381 tactgctgct ttcaccaaat ccttagtgct tctttcgtgt ttttctattg tcataatggt
     1441 tatctctaaa aaagaggtaa gatgcgtact acttactcgc cgttattggt attattcaga
     1501 aaaagtgagt aagactttgc agcaatgttt ttgatcctgt tcaaataaac taatggcatc
     1561 agcaacatgc tggaaatcaa acgtatgggt aattaatttt tctggtttaa ttaacccttt
     1621 acttaaccag tcgataacga ccggaaattt atttgcattt aagcgtgaag agaaaataga
     1681 gagttctttt ccggtaattc cttgctgaat cacttcagaa ggttcactgg agaagcccat
     1741 caatacaata cgtgccgctg gagaagccag cgttacggcc tctttcagga tagaaggatg
     1801 acaagccgca tcgataatta atgtcggctt tatgcctttt tcagcgaaaa tctcgccaag
     1861 cggtgtctgg ctgttattaa tcacccaatc agccccgctc tctttcgctt tttccagtcg
     1921 ttcatcaatg cgatcggcaa caatcacatt tttaacgtta tagacgcctt ttaatacctg
     1981 aacgatcgtc aggccgattg gaccggcacc ataaaccaga acggtatcat tttcagtcgg
     2041 ttgaccatgt ccggttacgt tagccgcaat ggtaaaaggt tcgatcatca ccgcatattg
     2101 atcggccact gcttcaggaa ttttccacgc attttttgcc ggaaccacag catattcact
     2161 gaaaccaccg tcaacatgca cacctaatac agcgagtgtc gtacaaacgt tcggcttacc
     2221 tatagagcac ggatagcaat gaccacagct gaccaccgga tcgacagcaa cacgttcacc
     2281 gactctggcg ctttccacac cttcacccac cgcatcaatg acgccaaaga attcatgtcc
     2341 aatgacgcgc ggatatttcg caaaaggatt atgcccacga taaatatggc tatctgaacc
     2401 acaaattccg gcaagtttta cttttactcg aacttcaccc gctgacgggg tgggtatttc
     2461 acgttcgata atcgccagtt gattcggttt ttcaattaaa atgcttttca ttaacttact
     2521 ccttaccagt tccacagcgt gccatcttcc agacgtgcga ctggtagata agcaggttca
     2581 tagggatatt tcgccgccag cttttcatcg aattcgatgc caagacccgg tttgtctccc
     2641 ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag catttgttcg
     2701 gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa gtgcagcgcc
     2761 gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg tacctgatac
     2821 aacgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg ggtcagcgtg
     2881 gtgcggatat aatcgataag ttgctcttca atcagttgct tgcagtccca gatgctgttg
     2941 aagacttcac ccaccgcgat gggtgtgatg gtatgttggc gaatgagacg gaagcattcc
     3001 tggttttccg caggcgtcgg gtcttccatc cagaacatgc gataatcttc aatgctttta
     3061 ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag caaatgttca
     3121 ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc gaggtatttc
     3181 tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc ataagccaga
     3241 cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggatgccgca ctgcacgcgg
     3301 atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc atcaatactg
     3361 tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc gagtaactgg
     3421 taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc aaccgctgaa
     3481 atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa aaactgccag
     3541 atatcttcga tacggtgcgc atcgcgacca ataagctgcg gacaaaggtg atcctgcaaa
     3601 taagaggcca cagaaagctc acgtccattg agggtggcat ccccaaggcc cgtaataccg
     3661 tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac aaaaacttca
     3721 gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat gaaatctacg
     3781 caactgagct actaccatac aagtatgaag atcaaaaaaa gccggagtga tcacaaaaaa
     3841 aggcgtatat tttcgccatg aatggttgac aaaagatgaa atagagtacc ttttatcagc
     3901 tgacgcttcc tcgtatctta ttgataaaat agatttatgt ccttacgtgc gcccccagcc
     3961 ggcaacaatg atcaacatgc cgcaaagcgc aatcaacgca cccgtccagt cataaagagt
     4021 cagtttcacg ccatccacaa cgcgcagcca catcaacgcc gtgcagacat aaacgccacc
     4081 ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc agacaaacag
     4141 cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt ttaaccacaa
     4201 ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata gtaacgttgt
     4261 tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca taatatcatc
     4321 aatataaaca ccctcctggt acgttttgcg ttcgcagtgg atggtgtaga atcaccttta
     4381 atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc ggcctgctcg
     4441 cttttctgct gccttgcaca ctggcattga gcacaactgt tcatgccgaa actaacaaac
     4501 tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg gaaaaagagc
     4561 aatggaatga cacgcgcaat ctgcgtcaga aagtgaataa acgcactgaa aaagagtggg
     4621 ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc aacatcaatg
     4681 cctactggga gccaaatact ttgcgctgcc tggaccgtcg aactggccgc gttattaccc
     4741 cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc ccacagtgtt
     4801 aagctacgcc cgctggagcg tgaagattta cgctatgtac atcaactcga caataacgcc
     4861 agtgtgatgc gttactggtt tgaggaaccc tacgaagcct ttgttgaact ctctgatctg
     4921 tatgataagc atattcacga tcagagcgaa cgacgctttg tggtggaatg tgacggcgaa
     4981 aaagccggtc tggtggagtt ggtggaaatt aaccatgtac atcgccgcgc agaatttcag
     5041 ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc caaattagca
     5101 atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt tgataaagag
     5161 aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg tgaattgatg
     5221 catgagttct ttattaatgg tcaatatcgt aatgccattc gcatgtgtat attccagcat
     5281 cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac cgcacaatag
     5341 cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc gggttttact
     5401 ccttcatcaa caatgatcag ctgacagtcc accggattgg cgtgactgtc atattcacag
     5461 ctctgtttta cattaccaac tcgttgatta ttcagtaaag taaccgctgt gtaatctaat
     5521 tttttgatcg ggtccgttga tggcgtggcg ctgacagata atgttttgtc gttacttttc
     5581 gtggttttac ccagtggata accctgatca tcatagcgat attccatctg catttgtttg
     5641 ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc ggcagaaggt
     5701 aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt ctcggcatca
     5761 cggtaataat tggcgtccag taccagagcg accacggtat tattttccag atccagtaat
     5821 tcgagtgaat caaaacagcc ttcttccgac aaagtccccg aaacacgttt cgtcacttca
     5881 ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag cggatcaaaa
     5941 tcgaattcat tagagaaact ggccatctca ggagtaaatg aaagtgttac ctctgtgcgg
     6001 tcacatcctg tgagtaatat cgcgagtaag catggcagta atttgtattt cacatcagtc
     6061 accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta cacgttaaat
     6121 gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct caacctgctg
     6181 tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg gatcctgtga
     6241 gcgcatacaa agcgatatat cacagcgcat tatcaataat ggcgtacccg aaagcagctt
     6301 cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg tcgtcgggca
     6361 ttgcgctaat gatacgcata aaattctcta cacccgcacc accagcggta acgtctctgc
     6421 tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaataca ttatcgactg
     6481 aacgccggat acgacgcatc cggcgatttg aacatataac ataacccacc ttattactca
     6541 tacccttcta ttg
//
LOCUS       DAZKFU020000250         6481 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZKFU020000250 DAZKFU020000000
VERSION     DAZKFU020000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.252"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(690..938)
                     /locus_tag="ACHOQQ_RS20405"
                     /old_locus_tag="pgaptmp_004081"
     CDS             complement(690..938)
                     /locus_tag="ACHOQQ_RS20405"
                     /old_locus_tag="pgaptmp_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4472271.1"
                     /translation="MRVEICIAKEKISKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIH"
     gene            complement(1164..1259)
                     /locus_tag="ACHOQQ_RS20410"
                     /old_locus_tag="pgaptmp_004082"
                     /pseudo
     CDS             complement(1164..1259)
                     /locus_tag="ACHOQQ_RS20410"
                     /old_locus_tag="pgaptmp_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1256..1822)
                     /gene="yecD"
                     /locus_tag="ACHOQQ_RS20415"
                     /old_locus_tag="pgaptmp_004083"
     CDS             complement(1256..1822)
                     /gene="yecD"
                     /locus_tag="ACHOQQ_RS20415"
                     /old_locus_tag="pgaptmp_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4472272.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLHRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            2132..3904
                     /gene="aspS"
                     /locus_tag="ACHOQQ_RS20420"
                     /old_locus_tag="pgaptmp_004084"
     CDS             2132..3904
                     /gene="aspS"
                     /locus_tag="ACHOQQ_RS20420"
                     /old_locus_tag="pgaptmp_004084"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGU4472273.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4022..4474
                     /gene="nudB"
                     /locus_tag="ACHOQQ_RS20425"
                     /old_locus_tag="pgaptmp_004085"
     CDS             4022..4474
                     /gene="nudB"
                     /locus_tag="ACHOQQ_RS20425"
                     /old_locus_tag="pgaptmp_004085"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGU4472274.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            4503..5243
                     /gene="yebC"
                     /locus_tag="ACHOQQ_RS20430"
                     /old_locus_tag="pgaptmp_004086"
     CDS             4503..5243
                     /gene="yebC"
                     /locus_tag="ACHOQQ_RS20430"
                     /old_locus_tag="pgaptmp_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4472275.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            5278..5799
                     /gene="ruvC"
                     /locus_tag="ACHOQQ_RS20435"
                     /old_locus_tag="pgaptmp_004087"
     CDS             5278..5799
                     /gene="ruvC"
                     /locus_tag="ACHOQQ_RS20435"
                     /old_locus_tag="pgaptmp_004087"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGU4472276.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(5801..6403)
                     /gene="yebB"
                     /locus_tag="ACHOQQ_RS20440"
                     /old_locus_tag="pgaptmp_004088"
     CDS             complement(5801..6403)
                     /gene="yebB"
                     /locus_tag="ACHOQQ_RS20440"
                     /old_locus_tag="pgaptmp_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HGU4472277.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1654 a         1527 c         1692 g         1608 t
ORIGIN      
        1 tgtgattata gtcataaact ataaaaatat tgatgttgtc tttgtagaaa ggttgtgagc
       61 aattatgaaa atttatttgc ttgttcactg gttttaggga gtttgatgtg tccatgaagc
      121 gtttctgacg agaaatcaac ataaatcacc agttggtgaa ctaggattgc cagaaacgct
      181 cctgtcagtt ctgtagtgtg tagtgctcac ttagagcctg gtgaactgga taatgagtcg
      241 cacctcagta tcctgcaatg ggctgctttt cccctgctcc tgattctttt ttgtttcagg
      301 tcttaccaac ataatggttg ttgtaataaa ttgcgtgtga ttctgtatcg ggtcaaccat
      361 gtgacgatac atgttcagta aggaagactg aagggttcag agtgcgagta tgattaatag
      421 atggaatcag cgtttcaaag attttgaggg aatataaggt ctgacctgat gattaccttt
      481 tgcgcctgta tcctttgcat aaaggaggtt gatgtagata aagtcatctc cacttgtgat
      541 ataaaacagt cagttatgaa aaatcggcat aaacatcctt ttgttaacct taaagccaga
      601 cttacggttt ttccatagtt acagataagc ggctagtaag gttttacagg tgataagcct
      661 gtaacggaaa tcacctgcaa atctattaat tagtgaataa accagtcatc cgctgtttcc
      721 cagacttcct gaagcgtttc ttgtacaaac tcttgcgcgg attctttatc cacggctctt
      781 aaaatagtga gtccatcatt gctggcagat ttaacgataa catctacatc gtcataacgc
      841 ttaccgacac gtcgtagcat ttcctgttgc agggcaggaa cagaaccttt tggcattttg
      901 ctgatttttt ctttagcgat acagatctca acacgcataa caccctcctg taactgtggt
      961 tatgtacagg tgttattttt atctgtatgg ataaccagtg tcaattcctg atgttgttta
     1021 tggggcatca atggggcatg tatgggacac tttttatcgg cgaaattcgt cgaagttcgt
     1081 cgacatggta aacgaatcat ctatccaacc cttgaaaaac ggcgctcctg gacgatcttc
     1141 gtcgattttt aaaaatgttg cgtcacgcag ttaaagtggc gggcatactc ttcaaggcgg
     1201 gtgatcccca accgcaccca tttaggatgc gaccattgcg gtagaccgat gtaaatcata
     1261 acgcgtgaag gatctcttcc acgctacgca cacgggcgat gcgcgggtag atatgattaa
     1321 tgctgttatt gtgttgctcg gcgctggcgg cactacaggc gtcttcggca atcaccagat
     1381 taaagccaag ttcccaggca ttgcgggcgg tggattcaac accgatattg gtcgagatcc
     1441 cacataacac tattgtatcg atacctcggc ggtgtaattg caactccaga tccgtaccgt
     1501 aaaacgcacc ccactgacgt ttgattattt cgatatcact gtcagttgca cctaatgcag
     1561 ctggatgttg ccaccagttt tcgggcaaca ctttagcggg ggaaggggca tcaaccggct
     1621 gttttaatgc ttcggcgtaa tcggcagacc agccaacgcg caccagaaac acgggctgac
     1681 cgctggcgcg aaatttcgcc gccagcttcc cggcgcgatt aactacctca tcggcagtat
     1741 gtggaccacc ggcaaaaggt aagatgcctt cttgtaaatc gatcaccacc agcgcagtgg
     1801 ttttagcatt aagttcaagc atgatgactc ctgaaaaaac aaaatcgtgc ctcacacctt
     1861 aatgcgatta gccgatggcg gaacgacaaa ttttgttaat ttttatgagt tcgggcaaca
     1921 cctttatagc aaactggcgt agagtctgat ttcaggcccg atataaagtg gtaacgaggc
     1981 gttccagcgc gggccaggct cttacggtgc ggcagaattt ccagtataat agccgccttt
     2041 tttcattcag ttgtgacata cagctaacgc tgcgacttgg tcacctgcgc agcagggggc
     2101 atccgccggc agctaagtta agggatatct catgcgtaca gaatattgtg gacagctccg
     2161 tttgtcccac gtggggcagc aggtgactct gtgtggttgg gtcaaccgtc gtcgtgatct
     2221 tggtagcctg atcttcatcg atatgcgcga ccgcgaaggt atcgtgcagg tatttttcga
     2281 tccggatcgt gcggacgcgt taaagctggc ctctgaactg cgtaatgagt tctgcattca
     2341 ggtcacgggc accgtacgtg cgcgtgacga aaaaaatatt aaccgcgata tggcgaccgg
     2401 cgaaatcgaa gtgctggcgt cctcgctgac tatcatcaat cgcgcagatg ttctgccgct
     2461 tgactctaac cacgtcaaca ccgaagaagc gcgtctgaaa taccgctacc tcgacctgcg
     2521 tcgcccggaa atggctcagc gcctgaaaac ccgcgctaaa atcaccagcc tggtgcgccg
     2581 ttttatggat gaccacggct tcctcgatat cgaaactccg atgctgacca aagccacgcc
     2641 ggaaggcgcg cgtgactacc tggtgccttc tcgtgtgcac aaaggtaaat tctacgcact
     2701 gccgcaatcc ccacagttgt tcaaacagct gctgatgatg tccggctttg accgctacta
     2761 tcagatcgtt aaatgcttcc gtgacgaaga cctgcgcgct gatcgtcagc ctgaatttac
     2821 tcagatcgat gtggaaactt ctttcatgac cgcgccgcaa gtgcgtgaag tgatggaagc
     2881 gctggtgcgc catctgtggc tggaagtgaa gggcgtggat ctgggcgatt tcccggtaat
     2941 gacctttgcg gaagctgaac gccgttacgg ttccgataaa ccagatttgc gtaacccgat
     3001 ggaactgacc gacgttgccg atctgctgaa atctgttgag tttgcagtat ttgcaggtcc
     3061 ggcgaacgat ccgaaaggtc gcgttgccgc tctgcgtgtt ccgggtggcg catcgctgac
     3121 ccgtaagcag atcgacgaat acggtaactt cgttaaaatc tacggcgcga aaggtctggc
     3181 ttacatcaaa gttaacgaac gcgcgaaagg tctggaaggt atcaacagcc cggtagcgaa
     3241 gttccttaat gcagaaatca tcgaagccat cctggatcgt actgccgcgc aagatggcga
     3301 tatgattttc ttcggtgccg acaacaagaa aattgttgcc gacgcgatgg gcgcactgcg
     3361 cctgaaagtg ggtaaagacc ttggtctgac cgacgaaagc aaatgggcac cgctgtgggt
     3421 tatcgacttc ccgatgtttg aagacgacgg tgaaggcggc ctgacggcaa tgcaccatcc
     3481 gttcacctca ccgaaagaca tgacggctgc agagctgaaa gctgcaccgg aaaatgcggt
     3541 ggcgaacgct tacgatatgg tcatcaatgg ttacgaagtg ggcggtggtt cagtacgtat
     3601 ccataatggt gatatgcagc agacggtgtt tggtattctg ggtatcaacg aagaggaaca
     3661 gcgcgagaaa ttcggcttcc tgctcgacgc tctgaaatac ggtactccgc cgcacgcagg
     3721 tctggcattc ggtcttgacc gtctgaccat gctgctgacc ggcaccgaca atatccgtga
     3781 cgttatcgcc ttcccgaaaa ccacggcggc agcgtgtctg atgactgaag caccgagctt
     3841 tgctaacccg actgcactgg ctgagctgag cattcaggtt gtgaagaagg ctgagaataa
     3901 ctgatatgag tcaaatacac gaaatcattc gcgttgcatc gaggcggcaa ctgagtgaac
     3961 tcccatgagc atagataact atgtgaatag gatgagcgaa ggcagtcaac gaagaggcag
     4021 cgtgaaggat aaagtgtata agcgtcccgt ttcgatctta gtggtcatct acgcacaaga
     4081 tacgaaacgg gtgctgatgt tgcagcggcg tgatgacccc gatttctggc agtcggtaac
     4141 cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc atgcgcgaag taaaggaaga
     4201 ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt gactgtcagc gcacggttga
     4261 gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg ggcgtgacgc gtaatacgga
     4321 atcatggttc tgtcttgcgc ttccgcacga gcggcagatc gttttcactg aacatctggc
     4381 ttacaagtgg cttgatgcgc ctgctgcggc ggcgctcact aagtcctgga gcaaccggca
     4441 ggcgattgaa cagtttgtaa ttaacgctgc ctgaacaggc agggctatct ggagatattt
     4501 ttatggcagg tcatagtaaa tgggccaaca ccagacatcg taaagctgcg caggatgcta
     4561 agcgcggtaa aatcttcact aaaatcattc gtgagctggt aaccgcggct aagctgggcg
     4621 gtggcgatcc ggacgctaac ccgcgtctgc gtgcagcggt ggataaagca ctgtctaaca
     4681 acatgacccg tgacactctg aaccgcgcaa ttgcacgtgg tgtgggcggt gatgatgatg
     4741 cgaacatgga aaccatcatc tacgaaggtt acggtcctgg cggcacggca atcatgattg
     4801 aatgtctgtc tgacaaccgc aaccgtaccg ttgctgaagt gcgtcatgca ttcagcaaat
     4861 gtggcggtaa cctcggtact gatggttccg tagcctatct gttcagcaaa aaaggcgtca
     4921 tctccttcga gaaaggcgat gaagacacca tcatggaagc agcactggaa gcaggtgctg
     4981 aagacgttgt gacctatgat gacggcgcga ttgatgtcta cactgcatgg gaagaaatgg
     5041 gtaaagtgcg cgacgctctg gaagcggcag gtctgaaagc agacagcgcg gaagtttcca
     5101 tgatcccgtc taccaaagct gatatggatg cagaaaccgc accgaaactg atgcgtctga
     5161 tcgatatgct ggaagattgc gacgacgtgc aggaagttta ccataacggt gaaatctctg
     5221 atgaggtcgc agcgactctc tgatgaggcc tgctaaacag caaaacggag acgcgtgatg
     5281 gctattattc tcggcattga tccgggttcg cgcgtgaccg gctacggcgt catccgccag
     5341 gtaggtaggc aactgtccta cctgggtagc gggtgcatcc gcaccaaagt ggatgattta
     5401 ccgtctcgtc tgaaactcat ctatgcgggc gtgacggaaa tcatcaccca gttccagcct
     5461 gattatttcg ccattgaaca agtctttatg gcaaagaacg ctgactcagc cctgaaactg
     5521 ggccaggcgc gcggcgtggc gattgtggcg gcggtgaatc aggagttgcc agtatttgaa
     5581 tacgcggcac gtcaggtaaa gcaaacggtg gtaggtattg gtagtgccga aaaaagccag
     5641 gtgcagcata tggtccgcac cttgctgaaa ctgcccgcta atccacaggc ggatgccgcc
     5701 gatgcgctgg cgattgccat cacccactgc cacgttagtc agaatgcgat gcagatgagc
     5761 gaatcgcggc tgaacctggc gagagggcga ctgcgttaag ttataccgcc tcggtcagtt
     5821 ccggctgagg cgtttccact ccctccgcat gaatcaacac caaacccgga tgatgccaca
     5881 aactggctgg cgtgacggtt ttacgctccc acggaataga acccaggaac cagaatttcc
     5941 agaaagtgag ttttgcattc ggattgctat ttaacaattc tccaaacgtc tctatttcac
     6001 ccaccggaat acatagcgct tctttataaa tatcaaaaac aaattttgaa cagaactggc
     6061 gcgaagattc gtatttaaaa ccggtgtggt aaagtttgcg tagccgggag ggaacctgtt
     6121 caacaattcg ttgtttttgt tgttctgtca gcccagcgtc taatcgcttt atagcatagc
     6181 gttgattaga cgaacgttta ataaaacgag atagcgtggt gatggttgaa aggggaaccc
     6241 ggctttctgc aaccaggaag tcttcaccgt tatgaccgat aatgatcccg acgtgattac
     6301 tccagcaatt tgatgcagct gatatttgac caaataaggc agcacctata catgtaaaga
     6361 cgatatctcc aatttcatat tcagcagggt aattaatatt cacgttgtca ttccattgaa
     6421 atagatacga atatacgtaa tagcataaaa aatttatttt ttcaggaggc gcaatgtata
     6481 t
//
LOCUS       DAZKFU020000251         6449 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZKFU020000251 DAZKFU020000000
VERSION     DAZKFU020000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.253"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2764
                     /locus_tag="ACHOQQ_RS20445"
                     /old_locus_tag="pgaptmp_004089"
     CDS             <1..2764
                     /locus_tag="ACHOQQ_RS20445"
                     /old_locus_tag="pgaptmp_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4472278.1"
                     /translation="DWMVNGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTVILAQK
                     ADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIAFNRLAA
                     ADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMDGGADI
                     SGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESRTFSI
                     GQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGTSAVG
                     GASGSSTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTSSALTV
                     SDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGSGEYDF
                     MTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFHTLTA
                     GSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLVTAPA
                     DTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQDAETPN
                     PVEAVSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVNSSAV
                     RDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNYTH
                     VQVGADKKHELDGLDLFTGVTMTYTYSNAGSDTFSGKTKSVGAGLYASALFDSGAHID
                     LIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAELVYGAV
                     SGKQFSWKDQGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLAN
                     GETVLRDASGEKRIKGGKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDNAVNA
                     SFRYSF"
     gene            complement(3331..6003)
                     /gene="rcsD"
                     /locus_tag="ACHOQQ_RS20450"
                     /old_locus_tag="pgaptmp_004090"
     CDS             complement(3331..6003)
                     /gene="rcsD"
                     /locus_tag="ACHOQQ_RS20450"
                     /old_locus_tag="pgaptmp_004090"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGU4472279.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVNIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         1571 a         1471 c         1794 g         1613 t
ORIGIN      
        1 tgactggatg gtgaacggac ttgcgggaga cgcacttcat aaaattggta agggaacact
       61 ggtggtggcc ggcagtggtg aaaatcctgg caccctgaat acgggggacg gaacggtcat
      121 tctggcacag aaggctgatg ctgcaggcag ggtccgggca ttcagtgaag tcagtattgt
      181 cagcggacga ccagttgtgg tgctgcagga cagtcatcag attgaagggg accgcattcg
      241 ctggggatac cggggaggta cgcttgatat caacggaaat gacatagcct tcaaccgcct
      301 ggctgcggct gatgaggggg cagtactcac aagccgggcg cgtcctgcca cagtccgtct
      361 ggatttcagc cagtcagggc agaaggccgt gatgtggcat ggtcatttta ccggtaacct
      421 cagcgtcctg aacaacacgt catctgcggt ggattttatc atggatggcg gagcggatat
      481 atccggcagt tttacacagc agggcggggg actgtatatc caggggcatc cggttgttca
      541 tgctgtcagc agtgaggcgg ttgcgactgc attgcgaaag cagggtgata attctgttct
      601 gacgcagccg gtatcatttg ctcagaagga ctgggagagc agaacgttca gcatcggtca
      661 actgaaactg aagggggcgg cattcagcct ttcccgcaat gcgacactga cgggagatat
      721 tgatgctgat aatgccacca tggtgctggg aagtgactcg ctatatctgg atatgaagga
      781 cggcacgggg agcagcagtg cgccggttaa ggggacgtct gccgtgggcg gtgcatccgg
      841 tagcagtaca tttcgcggga atgtgaatat gcgtcattct gccctgacgg tccgcgacca
      901 ttttactggc agtataacgg catcggacag ccggatagct gtgagttcag agaatgtgcg
      961 tctggaaggg aattcccgcc tgacctcttc tgcacttact gtcagtgacg gcgggcgttt
     1021 acatgtgaag gggggactgg agacagatgg tggtgtgaca ctggacagag gaaccctttt
     1081 ggtggacggg ggaagtgtca ggaatgatgt atatgaacgt ctgctggcct ggtcagagga
     1141 gcgtggcggg ctgaacggaa gcggtgagta tgattttatg acgggggcag ctggtctcct
     1201 gcggagttat gtcagaggga gtgcaggtaa tgtgaatctg cagaatgcgg cctggatgat
     1261 gacgggaaac tcatccgtga agcatcttga gtcatcggga agtgctctgt atttcagcag
     1321 gccgggcggg gagttccata ccctgacggc aggcagtatg gatatcagcg acagtgttct
     1381 ggtgatgcgg acggacctta accactcaga ccagcttcgg gtgacagaat cgctgcgggg
     1441 taagaataac ctgttgttgg ttgattttac tgagcgttct gacgggcaga aggcgctgaa
     1501 tatccctctg gtgacggcgc cggccgatac cggtgcggat gtgttcagtg tgaaaacgcg
     1561 tgataccggc ttcagtcata tcacacctgt tgtcagggct gaacagggga cgggcggcac
     1621 ggcctggcag ctgaatgttg tgcagccgga aactgccgca gagcctgtgg cgacgcggca
     1681 ggatgcagag acgccgaatc ctgtggaagc tgtatcaccg ctcccttcac cggtttctgc
     1741 tccgtcatcg gctccggagg cgtcagtgac ggatgtactg accggagaga atgcggggga
     1801 atcaggtgag taccgtgatg agacccggtg gctgctgaca ggataccgga gtacggttaa
     1861 tagctccgcg gtcagggatg cggggatgct gatgtcaatg gggcaccgta acttcattaa
     1921 tgaggtcaac aacctgaaca aacgtatggg tgacctgcgg gacatcaacg gcgaggccgg
     1981 tgcatgggcc cgtatcatga gcggaaccgg ttctgccggc ggtggcttca gtgacaacta
     2041 cacgcacgtt caggtgggtg cggataaaaa acatgaactg gacggtctgg acctcttcac
     2101 cggggtgacc atgacatata cctacagcaa cgccggcagc gacaccttca gtggtaaaac
     2161 gaagtcagtg ggtgcaggtc tgtacgcttc agccctgttt gattccgggg cccatatcga
     2221 cctgattggt aagtatgttc accatgataa tgagtatacg gcgtcctttg ccggactcgg
     2281 cacaaaagac tacagctctc attcctggta tgccggtgct gaagtgggtt accgctatca
     2341 tgtcacagag gatgcctgga ttgagccaca ggcggaactg gtctatggtg ctgtatccgg
     2401 taaacagttc agctggaagg accaggggat gagcctgtcc atgaaggaca aggactacaa
     2461 tccgctgatt ggccgtaccg gtgtggatgt gggtaaatcg ttctccggta aggactggaa
     2521 agtgacggca cgtgctggtc ttggctacca gtttgacctg ctggcgaacg gagaaacggt
     2581 actgcgtgat gcatccggag agaagcgtat taagggcggg aaagacggcc gtatgctgat
     2641 gtctgtgggt ctgaacgcag aggtccgtga caacatccgt tttggtctgg agtttgagaa
     2701 atcagccttt ggtaagtaca acgttgataa tgctgtcaac gccagcttcc gttattcatt
     2761 ctgacggttg ttactgaagg ggattatggc ctgacggctc cctgtaaata aagacaggca
     2821 cagaagattg tgagttactt agtgttccac caacaagtaa ggtcgtaact ctgtgcctgc
     2881 ttcgtaagcg tacagtgagg gccatcgtca taactgaagc atccgacgac gtggtgtcta
     2941 ccagagtgtc agatatgcag gaaaatatga gctccgacag aaatgcagta aaaaaagaga
     3001 gtctcgtaca cccctccaga aaactccagg gctgtaagtt gtattaaaaa gctgattttt
     3061 ctttagcgat acagacctca ataagtataa cacccttcag ttactgtggt tatgcacagg
     3121 tgttattttt aactgtgtgg ataaacagtg tcgattattc agattggtta tggggcatgt
     3181 atgggacatt tttaatcggc gaaatacgtc gaagttcgtc gatatggtaa acgaatcatc
     3241 tatccaaccc ttgaaaaacg gcgctcctgg acgatcttcg tcgattttta aaatgcaaca
     3301 agtacgttgt tcatgtaata ggctaccttg ctacagcaag ctcttgacat aactgtcaat
     3361 gtcgctgatg tatttttcta ttcctggaac atccttctca cgaatcagat gttccagcgt
     3421 ttcacataac tgcttgccgg gtaccagatt tagcatggca aatacgcctt taagacgatg
     3481 agccgtttgg gctaacgcag caaagtcact ggttgctgct tcagtataca gcctcttaac
     3541 atcatccggt actgtgtcta caaagagcgc ataatagccg ctggcatgga gttgcgcatt
     3601 ttcatctccg cccagaggcg attctgtcac ctcttcctgc gccagttgca cttcaattaa
     3661 ttgtaagacc gcttcctgca tagcgttgct catattgaag ttgacgcaca attgaccagg
     3721 cccaatttcc cgtacgccag actcatcatc gcttaaaagc aagccagagg cagtaagatt
     3781 agacggatta tccgttaaaa agatatcata atcttgacta attaatcttt catcgggtgt
     3841 gatacaggtt gcaccccaat tttctaactg gcgagtgaca atattccgaa tttctgccga
     3901 agtaacatcc accattacgc agacatcatc cagtaaacgc tcttcttcct cttcaacttc
     3961 cgggtcagct gcgagcattt tgatatgcac agagtagcgt gtaccaagcc catcccgcgt
     4021 tttgatgttt aaatgaccgc ccagtttacg tgccagttga tcgctcagcc agaatgccag
     4081 cgggtccgcc ttgccatagc gatcgttttg ggtctggttg ataaacggga agtgcaaatt
     4141 atccatttca tgaatgttta cgccttctcc ggtgtccagg atgcggaacg tcaggcggtc
     4201 ttcggaggac tcatcctgat caacctcaag ggtgattttt cccaattgcg ttgaggtcac
     4261 ggcatattgc atcagtagca gcaaaatacg tcgtaaggca tcgcgatcgc cgcggcgcat
     4321 atcgtgtgct ttcagatgat tgttaatcag cagttgcaga cctttacgct tgatggcagg
     4381 caacactgaa ggcacaactt catcaattaa atcctgcacg gagaacagca ccgtctcact
     4441 tttccagcta tcgtccgcaa gcatgttcgc taactgtatt tcatcgacca gccgcaccag
     4501 cacatctgcc tgattcgcca gttgtttgct ttccggggcg ttgagtttag ccgcgctctc
     4561 cgccagggac tgtgcaggtt ctttcagcgc atcgccaatg tttttcataa aggtcatccg
     4621 cccctgctgg tttttctcat acagacgctg cgcctgcttg agtttcttgt ttaccagcac
     4681 ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc ggcgcgacct ggctgcggaa
     4741 catgcggatc tcatacagct cgttattgat cgtcgcctga ataatcccct gatgctgttc
     4801 cgccatggtg gtgatgtttt gcagattcaa atgcggcagc aaatgatcgg caattttgtt
     4861 acttatgaca gtgcggttcg attcctgatc gtgaaccagc aggccgagcg gcagcagtga
     4921 gactatctct tcattgattg cccgtaaaat gcgcaattcg ttattgaccg ccgtgttggg
     4981 tagactttct gtactgcgac tggagaaatg gcggaatgtg gtatagccaa ataacgccag
     5041 cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg ttttgcaacg tatccagcaa
     5101 taaggtgcca taaggaacct gccagaccag gcgcatatcg gtagagttga gtgccgagga
     5161 gatttcaatc ttcgtactgt taaagtggat actgacgcta tccgtccctt ctttctcatt
     5221 atcattgtct cccgttgccg tcgcgtctgg ctcaaggcgg aaactgtcca gcggcatacc
     5281 cggtgggatc aaatcattaa tcggcagatc aaaagccacg accgttgcca gatgtcccgg
     5341 ctggttaaag gtggtacgca aggtaaagta atgaccgttc tgccaggcca ggcggcgcat
     5401 gttagaaaag ctttcgcgtt catcgagagc gttggcctgt tgcaacatct ctgcacgacg
     5461 tgaatcaaca atgtcactga cggtcgattc tttaaatccg gaggtgagat ctttgagggg
     5521 cagggttgag atcagcacca gactgttatc ctgaccattc aggtaataca ttgaccacgg
     5581 tacattttct gcgccccaca atgtgtccag ataggtggac atccgttgag tcatctcaag
     5641 cgttgagttg tcgtgagagc caaagattaa cgcttccgtt ttgcggcgcg gtttttccag
     5701 atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct tcgccggagg agggggaggt
     5761 cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa cgccaggtat cgacgcgctt
     5821 ttggatagcg tgggtaatgt cgacaatctg gtaactttta tctttcagcc aggcgttaac
     5881 ggcgctttgt accattacac ccatcgtcac cagtaacaca atgatcaaca gtaaaaagaa
     5941 gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg gccgttgtct ctttctgacg
     6001 cattcgtgtt tatgacctgt taaaacttcg cgaagtgata gggcagtata aagggtacag
     6061 tgtgaaattc cagactctta cctgattgat gattctctat tctcccgtga tatttatcat
     6121 cctgcattgg gggaaataga tgtacaagcg ccattttgct gtacaaataa cctacaaaaa
     6181 gcccaacgaa aataattgca aataaacaaa gattgctgga aattatgctg atgttaatta
     6241 tttgtgaaat ggttaacaag cgttatagtt tttctctggt agcacagaat aatgaaaagt
     6301 gtgtaaagaa gggtaaaaaa aaccggatgc gaggcatccg gttgaaatag gggtaaacag
     6361 acattcagaa atgaatgacg gtaataaata aagttaatga tgatagcggg agttattcta
     6421 gttgcgagtg aaggttttgt tttgacatt
//
LOCUS       DAZKFU020000252         6413 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZKFU020000252 DAZKFU020000000
VERSION     DAZKFU020000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.254"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1280
                     /gene="tssI"
                     /locus_tag="ACHOQQ_RS20455"
                     /old_locus_tag="pgaptmp_004091"
     CDS             <1..1280
                     /gene="tssI"
                     /locus_tag="ACHOQQ_RS20455"
                     /old_locus_tag="pgaptmp_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HGU4472280.1"
                     /translation="PGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQME
                     GWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRRG
                     AGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKFNW
                     DRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVVVDFLNGDPDQPIIMGRTYHQ
                     ENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGNEQLYMHAQKNMDTEVLNNRT
                     TDVKVNHTETVGGNQAISVTGVQSTSVKSAQFTHVESLRQVAVQQGQLIVTGGDHEIL
                     VQETLHLAAGKAIHLQCGESELVMLANGTVALSCTNFNLYAAQRGQITAAQILDLNMD
                     GAKSGIEQPGPLTPSAISERVTAEFPPQQPENK"
     gene            1280..1708
                     /locus_tag="ACHOQQ_RS20460"
                     /old_locus_tag="pgaptmp_004092"
     CDS             1280..1708
                     /locus_tag="ACHOQQ_RS20460"
                     /old_locus_tag="pgaptmp_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGU4472281.1"
                     /translation="MPDYHFNEGQITLPASGRDCTTHVLRLPELQAVLTISRDILSGT
                     MTLEEYVADQIAKIKRDARHVQIPEPQFTDMKNGKHVCQFSCQAEISGLVHYQQQHII
                     LSGQSLLAFAWTLGHPFTEQDHQTWRNILASFVPRETVTE"
     gene            1713..6353
                     /locus_tag="ACHOQQ_RS20465"
                     /old_locus_tag="pgaptmp_004093"
     CDS             1713..6353
                     /locus_tag="ACHOQQ_RS20465"
                     /old_locus_tag="pgaptmp_004093"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4472282.1"
                     /translation="MGTDYLAAKMEDPLEHTSVLGDFMGGLVEGVCYGVLFCASANPV
                     GLAVGLTAGFIFSDVISEIGDTVSSWFPPDVAGTITSGSKNVNVKSKPAARAAGAVPD
                     EILLALLEAEAANPPDSKGMQLVKGIVAKSIMIAKLPLLPAELGRMAGEKLAAYLKGE
                     STPSQEDEQGFWSRVWESISNPVVDGPDPALHVETRLLDTIDCHKHRPQPIQYLAEGS
                     ETVRINSQPACRNGDRSTCEAVIAHTQASAKVRIGGRSLVVKEIHTGKNMLAYYAGLI
                     AGGFLVSSVQALFCRTTAREFAGKLPCLMLGSVLGNTLGIVAGGTLSTSLSSLSSGLQ
                     ASSQTARPVHIASGVKILGGEDELDFACDGLIPLAWQRIYSSLNDNEGILGRGWCLPF
                     EVFLHQEPAVPGAQDSAQIFFHDMSGRELGLGDVPVGGAMFYVDEGFRLYHPLAGMFV
                     LESAQGQYQIFEADPLHDGRFRLIQECDRHLNGIRYYYNDRGLPVRIEDDLQAICLHL
                     DYHPQHSRLTAVYQQYRQEDTLPGVLRIRYEYNEQVQLTAVVDADGVVMRRFAWDEEH
                     GRLAMHQTADGLQAHYRWQPTSGLPGHFHVARHWLQEGERRSGEILFDYDPAARRATL
                     HYGDGRPDSLHEWDEQYNITRWQPAPDICWLYEWGDNRELLRVVDPAQCEYGFKYDER
                     GNLIQVVDPVGQEYRFDWDRDFAFPLKEEYPEGLVLRHVYNVSGDRIKTIDQAGLITR
                     YNHDASGLVESVQDARGNLYRYAWNNRGQLTRQTDCSGYTTHLYYDEYGRLAAVTDAQ
                     GNCTRQTFSAAGRLQKQIRPDGGETTCHYNARGQLAGVTDPARQSSRFDYNLRGQVIR
                     HTDPQQRQVRFEYDAGGQLIALYNQKNEAYRFSRDSLSRLEESCDLTGCREVYRYDSR
                     SHVSEITRYPAPAVSGQETFLPQVTRLAYDAAGRVQYRENGDGAVCYRYGKNTLSLER
                     FAPDDWQKKQRGYENIAAQDTLHLVTDAYGSLLEEHNPAGIFTHRYDELGNRTETQMP
                     DGRRLKRLYYGSGHLQQLNLEDNDGQQTVLAEFERNSLHQEISRTQGSLTLRTEYDAA
                     GRITARLAQMAQQRHGTPLLERRYSWDQRGFLMSRYHRTQDAQALLSPEERSGSTWYQ
                     YDCAGQILSAQQHFTVRQYCDLAGNLMEERSPAIKNNQLMQYRGWHYQYDAFGRMSRR
                     TDEQAQRQDYRYNSDNRMVEVRFADRTHSRWQRVEYDYDLLGRRTGKRLYRWHDEGEA
                     EPAPERVRFIWDGMRLRGEDSPGKATEVLYVYNGDSYEPLARVDRFTRQVIEPQDTRP
                     PKTTVYYYHTALNGLPEAMTSESGQLVYEARYELWGKETEAASAHTVLTVGQNLRFAG
                     QYFDAETGLHYNTFRFYAPECGRFTQPDPIGLRGGLNLYSYVPNPLSWIDPLGLKCWS
                     TEQKNYWKDVGAKELTNPSGLYSPRNILEMIAGRAPKFTARVKVIKTGKIETRDIPYE
                     LHHKNIPQRQGGPGVHNKSNLDEVDPWQHQDTDKYRHSGIELIEVITGVNSW"
BASE COUNT         1507 a         1626 c         1981 g         1299 t
ORIGIN      
        1 gccccggctg ggcaggacgt tttgatcagg aaggccagca ccaggattac cagcgcacgc
       61 agtatgaggt gtatgactac cccggacgtt tcaagggcgc ccacgggcag aactttgccc
      121 gctggcagat ggagggctgg cgaaacaatg cagaaaccgc gcggggaatg agtcgctcgc
      181 cggagatatg gccgggacgg cgaattgtgc tgacggggca tccgcaggcg aacctgaacc
      241 gggaatggca ggtggtggca agtgaactgc acggcgagca gccgcaggcg gtgccgggaa
      301 ggcggggagc agggacggcg ctggagaacc attttgcggt gatcccggca gaccggacat
      361 ggcgacccca gccgcggcta aaaccgctgg tggacggtcc gcagagcgcc gtcgtgacgg
      421 ggccggaggg tgaggaaatc ttctgtgatg aacatggccg agtgcgggtg aaattcaact
      481 gggaccgtta taacccggca gaccaggaca gctcgtgctg gatccgtgtg gcacaggcgt
      541 gggcaggcac cggttttggc aacctggcga taccgcgtgt gggtcaggag gtggtagtgg
      601 acttcctcaa cggcgatccg gaccagccga tcattatggg gcgcacctac caccaggaaa
      661 accgcacacc cggcagcctg ccggggacaa agacgcagat gaccatccgt tcgaagacgt
      721 atatgggcag cgggtttaat gagctgaagt tcgatgatgc aacgggtaat gagcagttgt
      781 acatgcatgc ccagaagaac atggataccg aggtgctgaa taaccggacc acggatgtga
      841 aggtaaacca taccgaaacg gtgggaggca accaggctat ctcagtgact ggggtgcaga
      901 gtaccagcgt taaatctgct cagttcacgc atgtggaaag tttacgtcag gttgcggtgc
      961 agcaggggca gttgatagtg accggcgggg atcacgaaat tctcgtacag gagacgctgc
     1021 atcttgccgc aggaaaagcc attcatctgc aatgcggtga atctgagctg gtcatgctgg
     1081 ccaacggtac ggttgccctg tcctgtacca atttcaatct ttatgccgcc cagagaggac
     1141 aaattaccgc agcccagata ctggatctga atatggacgg cgcaaagtca ggtatcgaac
     1201 agcccggtcc actgacgccg tcggctattt ctgagcgtgt caccgccgag tttcccccgc
     1261 agcaaccgga gaataaataa tgccggatta tcactttaat gaaggacaga ttacgttacc
     1321 ggcttcaggg cgggactgta ccacccatgt tttgcgactg ccggaattac aggctgtgct
     1381 gaccatctcg cgggacattc tgtcaggaac catgacgctg gaagagtacg tcgccgatca
     1441 gattgcaaaa attaagcgcg atgcacgcca tgtgcaaatc ccggagccgc agtttactga
     1501 tatgaagaat ggaaaacacg tctgccagtt ttcctgccag gcagagattt ccgggctggt
     1561 tcattaccag caacaacaca ttatcctgtc cggacaatcg ctgctggcct tcgcatggac
     1621 gctcggtcat ccgtttactg agcaggacca ccagacctgg cggaatatcc tggccagttt
     1681 tgtcccgcgt gagacggtaa cggagtaatg tcatgggaac agattatctg gcggccaaaa
     1741 tggaagatcc tcttgagcac acttctgtcc tgggggattt catggggggc ctggtcgaag
     1801 gggtatgcta tggggtactt ttttgtgcct ctgccaaccc tgttggactg gcggtagggc
     1861 tgactgccgg ctttattttc agcgatgtta tttctgaaat cggcgatacg gtcagtagct
     1921 ggtttccccc ggatgttgca ggtacgataa cgtcaggctc aaaaaacgtt aatgtgaaat
     1981 ccaagccagc cgcccgtgcg gcaggtgcag tgccggatga aatattactg gcactcctgg
     2041 aggcggaagc ggccaatcca ccggacagta aggggatgca actcgttaaa gggattgtgg
     2101 caaaaagtat tatgattgcc aaactacctc ttttgccggc agaactgggc aggatggcag
     2161 gcgagaaact tgccgcttac ctcaaagggg aaagtactcc gtctcaggaa gatgagcagg
     2221 gattctggag tcgggtctgg gaatccatta gcaacccggt ggtggacggc cccgatccgg
     2281 ctttgcatgt cgagacacgg ctgctggaca cgattgactg tcataaacat cgcccgcaac
     2341 cgatacagta tctggcggaa ggttcagaaa ccgtacgtat taacagccag cccgcctgcc
     2401 gtaacggtga tcgcagtacc tgcgaggcgg tgattgccca tacccaggcc agtgcgaaag
     2461 tccgtattgg cgggcgcagc ctggtggtga aggaaataca caccggcaaa aatatgctcg
     2521 cttactatgc agggcttatt gccggaggat ttctggtcag ctcagtgcag gctctgtttt
     2581 gcaggacaac ggccagggag tttgcgggga agttgccctg cctgatgctg ggttcggtcc
     2641 tgggcaatac gctggggata gtggccggag gaacactttc cacttcatta tcgtccctgt
     2701 cttcagggct gcaagcgtca tcacagaccg ccaggccggt acacatcgcc tccggtgtaa
     2761 aaattctggg cggagaggat gagctggatt ttgcctgtga cgggttaatc ccgctggcat
     2821 ggcagcggat ttacagcagc cttaatgaca atgaaggcat tcttggccgg ggctggtgcc
     2881 tgccgtttga agtgttcctg catcaggagc ctgctgtacc gggagcgcag gacagcgcgc
     2941 agatattttt tcatgacatg tccggcaggg agctggggct gggagatgtt ccggtcggcg
     3001 gagccatgtt ctatgtggac gaaggtttcc ggctttatca tccactggct ggtatgttcg
     3061 tgctggaaag cgcacagggg cagtaccaga tttttgaggc agatcccctg cacgatggtc
     3121 ggttccgcct gattcaggaa tgcgatcgcc atctgaacgg tatccgttat tactacaatg
     3181 accggggact gccggtgcgg atcgaggatg atcttcaggc catctgcctg catctggact
     3241 atcacccgca gcattcccgt ctgacggccg tataccagca gtaccggcag gaggataccc
     3301 tgccgggcgt actgcgtatt cgttatgaat ataatgaaca ggtgcagctc accgccgtcg
     3361 tggatgctga cggggtcgtg atgcgccgtt ttgcctggga tgaagagcac gggcgcctgg
     3421 ccatgcacca gacggcggat ggcctgcagg cgcattaccg ctggcagccc acttccggtc
     3481 ttcccggcca ttttcatgtt gcccggcact ggctacagga aggcgagcgg cgcagcgggg
     3541 aaatcctgtt tgattacgat ccggcggcgc gccgggccac gctgcattat ggtgatggaa
     3601 ggccggacag cctgcatgaa tgggatgagc agtacaacat tacccgctgg cagcctgccc
     3661 cggatatctg ctggctgtat gaatgggggg ataaccggga actcctgcgg gtggttgatc
     3721 ccgcacagtg tgagtacggc ttcaagtacg atgagcgcgg caacctgatc caggtggttg
     3781 atcctgtcgg gcaggaatac cgatttgact gggaccggga ttttgccttt ccgctgaaag
     3841 aggaatatcc cgaagggctg gtcttacgcc atgtttacaa tgtctctggg gatcgcatca
     3901 aaaccattga ccaggcaggc ctcatcaccc gctataacca tgatgcttcc gggctggttg
     3961 agagcgtaca ggatgcacgg ggcaacctgt accgctacgc ctggaataat cgtgggcagt
     4021 taacccggca gacggactgt tccgggtata ccacccacct gtattatgat gagtatggac
     4081 ggctggctgc cgtgacggat gcacagggta actgcacccg ccagacgttt tcagctgcgg
     4141 gccgcctgca aaagcaaatc cgccctgatg gcggggaaac cacctgccat tacaatgccc
     4201 gtggacagct tgccggtgtg accgatccgg cacgtcagag cagccggttt gactataacc
     4261 tgcgcgggca ggttatccgc cacactgatc ctcagcagcg ccaggtgcgc ttcgaatatg
     4321 atgccggcgg gcagcttatt gcgttgtata accagaaaaa tgaagcgtac cgtttcagcc
     4381 gggacagcct gtcacggctg gaagagtcct gcgatctgac ggggtgccgc gaggtctacc
     4441 ggtatgacag ccgcagtcat gtcagcgaaa taacccgtta tcccgccccc gccgtatccg
     4501 gtcaggagac gtttttaccg caggtcaccc ggctggcgta tgatgccgcc ggacgggtgc
     4561 agtaccggga gaacggagat ggcgctgtct gttaccggta tggaaaaaac acgctgtcac
     4621 ttgagcggtt tgcgccggat gactggcaga agaaacagcg cgggtatgaa aatatcgctg
     4681 cgcaggacac gctgcatctg gtcacggatg cgtatggcag cctgctggaa gaacataacc
     4741 cggcgggcat ctttacccac cgctatgacg aactgggcaa ccgtacagaa acgcagatgc
     4801 cggatggtcg ccgcctgaag cggctgtact acggcagcgg tcacctgcaa cagctcaacc
     4861 tggaagacaa cgacgggcag cagacggtac tggctgagtt tgaacgtaac agcctgcacc
     4921 aggaaatcag ccggacccag ggcagcctga cactgcgcac ggaatatgat gccgccgggc
     4981 ggattactgc ccgtctggcg cagatggcgc agcagcgaca tggaacaccg ttgctggagc
     5041 ggcgttacag ctgggatcag cggggcttcc tgatgagccg ttaccaccgg acacaggatg
     5101 cgcaggcact gctttccccg gaggaacgca gtggcagcac ctggtaccag tatgactgcg
     5161 ccgggcaaat actgagcgca cagcagcact tcacggtgcg gcagtactgc gatctggccg
     5221 gaaacctgat ggaggagcgc agtccggcga tcaagaataa ccagttaatg cagtaccggg
     5281 gctggcacta ccagtatgat gccttcgggc ggatgagcag gcgcacggat gaacaggcgc
     5341 aacggcagga ttaccgctat aacagcgata accggatggt ggaagtgcgg tttgcggaca
     5401 ggacgcactc ccggtggcag cgggtggaat acgactacga tctgctcggg cgacggacgg
     5461 ggaagcggct gtaccggtgg catgatgagg gcgaagcgga accggctccg gaaagggtgc
     5521 gttttatctg ggacgggatg cggctgcggg gtgaagacag cccgggtaag gccacagaag
     5581 tcctgtatgt ctataacggc gacagttatg agccgctggc cagggtggat cgttttaccc
     5641 ggcaggtgat agaaccgcag gacacgcgcc cgccgaaaac cacggtatat tactaccaca
     5701 cggcgctgaa cggcctgccg gaagcgatga ccagtgaatc agggcaactg gtctatgaag
     5761 cgcgttatga actgtggggg aaagaaacgg aggctgcaag cgcacatacg gtcctgacag
     5821 tggggcaaaa cctgcggttc gcggggcagt attttgacgc ggaaacgggg cttcactata
     5881 atacgttcag attttatgcg ccggaatgtg ggcggtttac ccagcctgac ccgatagggc
     5941 taagaggcgg gctgaacctc tatagttatg taccgaatcc gctgtcatgg atcgatccac
     6001 ttggtcttaa gtgttggtca acagaacaaa aaaattattg gaaagatgtt ggtgcaaaag
     6061 aattaaccaa tccatcagga ttatattcac caagaaatat tttagaaatg atcgctgggc
     6121 gtgcacctaa attcacagct agagttaaag taattaaaac gggtaaaata gagacaagag
     6181 atataccata tgagttacac cataaaaaca ttcctcaaag gcaaggtggg cctggtgttc
     6241 ataataagag taatttggat gaagtcgatc cttggcaaca tcaggatacg gataaataca
     6301 gacactcagg aattgaactt attgaggtta taacaggagt taactcatgg taattgataa
     6361 taactatatt ttattcggtg cggcattgtc cgagatagcg acgaattcat ata
//
LOCUS       DAZKFU020000253         6329 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZKFU020000253 DAZKFU020000000
VERSION     DAZKFU020000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.255"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1163
                     /locus_tag="ACHOQQ_RS20470"
                     /old_locus_tag="pgaptmp_004094"
     CDS             <1..1163
                     /locus_tag="ACHOQQ_RS20470"
                     /old_locus_tag="pgaptmp_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGU4472283.1"
                     /translation="FRPLTFESNFTHGHMQHIVSWDDKSELIIGQLHNIQGRQLFLMA
                     FHQSHTSFSVANAPDEPHIEQLVGECRVMRQLKRLISRIAPSPSSVMVVGESGTGKEV
                     VARAIHKLSGRRNKPFIAINCAAIPEQLLESELFGYVKGAFTGASANGKTGLIQAANT
                     GTLFLDEIGDMPLMLQAKLLRAIEAREILPIGASSPIQVDIRIISATNQNLAQFIAEG
                     KFREDLFYRLNVIPITLPPLRERQEDIELLVHYFLHLHTRRLGSVYPGIAPDVVEILR
                     KHRWPGNLRELSNLMEYLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSL
                     DDAGGTALEEMEKQMIREALSRHNSKKQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1203..1682)
                     /gene="xdhC"
                     /locus_tag="ACHOQQ_RS20475"
                     /old_locus_tag="pgaptmp_004095"
     CDS             complement(1203..1682)
                     /gene="xdhC"
                     /locus_tag="ACHOQQ_RS20475"
                     /old_locus_tag="pgaptmp_004095"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGU4472284.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(1679..2557)
                     /gene="xdhB"
                     /locus_tag="ACHOQQ_RS20480"
                     /old_locus_tag="pgaptmp_004096"
     CDS             complement(1679..2557)
                     /gene="xdhB"
                     /locus_tag="ACHOQQ_RS20480"
                     /old_locus_tag="pgaptmp_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGU4472285.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(2568..4865)
                     /gene="xdhA"
                     /locus_tag="ACHOQQ_RS20485"
                     /old_locus_tag="pgaptmp_004097"
     CDS             complement(2568..4865)
                     /gene="xdhA"
                     /locus_tag="ACHOQQ_RS20485"
                     /old_locus_tag="pgaptmp_004097"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HGU4472286.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVSRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5281..6036
                     /gene="actS"
                     /locus_tag="ACHOQQ_RS20490"
                     /old_locus_tag="pgaptmp_004098"
     CDS             5281..6036
                     /gene="actS"
                     /locus_tag="ACHOQQ_RS20490"
                     /old_locus_tag="pgaptmp_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGU4472287.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6115..6188
                     /locus_tag="ACHOQQ_RS20495"
                     /old_locus_tag="pgaptmp_004099"
     tRNA            6115..6188
                     /locus_tag="ACHOQQ_RS20495"
                     /old_locus_tag="pgaptmp_004099"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6147..6149,aa:Gly,seq:ccc)
BASE COUNT         1564 a         1557 c         1625 g         1583 t
ORIGIN      
        1 gtttcagacc attaacattt gagagtaatt ttacccatgg acatatgcag catattgttt
       61 cgtgggacga taaaagtgaa ttaatcattg gccaattgca taacattcag ggccgacaat
      121 tatttttaat ggcgtttcac caatcgcata ccagtttttc tgtagcaaat gcacctgatg
      181 aaccgcatat tgaacaattg gttggcgagt gccgtgttat gcggcaatta aaacgactca
      241 ttagccgtat tgcacccagc ccatccagcg ttatggtggt tggtgaaagc ggcacgggta
      301 aagaagtcgt cgcccgtgca atccataagt tgagcggaag acggaataaa ccctttattg
      361 ctatcaactg tgccgcgatt ccggagcagc ttctggagag cgaactgttc ggttatgtta
      421 aaggcgcatt tactggcgct tctgccaacg gtaaaacagg gttgattcag gcggcgaata
      481 cgggcacgct gtttctcgat gaaataggtg atatgccatt aatgttgcag gctaaattac
      541 tgcgcgctat tgaggcacgt gaaattctgc cgattggtgc cagtagccca atacaagtcg
      601 acattcgcat catttctgca actaaccaga atttggccca gttcattgcc gaaggtaaat
      661 tccgcgaaga tctcttctac cgacttaatg ttatcccgat aactctgcca ccgctgcgtg
      721 aacgtcagga agatattgaa ctactggtgc attacttttt acatctgcat acccgtcgtc
      781 tgggatcggt ttatcctggc attgctcccg atgtcgtcga aatattgcgt aagcatcgtt
      841 ggcccggaaa cctgcgcgag ttaagcaatt tgatggaata tctggttaac gttgttcctt
      901 caggtgaagt tatcgacagc acgctattgc cgccaaatct gctgaataat ggcacaacgg
      961 agcaaagtga tgtaacagag gtcactgagg cacacctgtc actcgatgat gcgggcggca
     1021 cggcgctgga ggagatggaa aagcaaatga tccgcgaggc gctttcacgt cataacagca
     1081 agaagcaagt tgctgatgaa ctgggcatcg gcattgctac gctctatcgc aagattaaga
     1141 aatatgagtt gttaaacaca taacatcagt gaatccggcc tgaattcagg ccggatatcc
     1201 ttttacttcg ttttctcgca atccagaact gtatttacaa tcatctgata ccccgtgcag
     1261 cgacaaagat ttcccgccag tccgcgacga atttccgtaa tggttaatgg cttctcgcgt
     1321 ggtttcgcca gcattgccgt ggtagccata atcaggccag gcgtacaaaa cccgcactgc
     1381 actgcgccgg atttcgcata agcctgctga acatgagaaa gttttccgcc tttcgcttca
     1441 ccttccagcg tgcggatctc ttttccttca gcccaggcgg caaggtataa gcaactgtct
     1501 attgctgtgc cgtcgaccag caccgtacag gcaccacatt cacccacgca gcacccttgt
     1561 ttgacactta gcagtccttg ttcgcggagt aattccgaga gcggcgtgcc tggtgcggca
     1621 tgaagctgaa aaggcatccc gttaatggtg cattcgatgg taattgtttc gctgtgattc
     1681 attgcaattt tccccccgcc gcggcgacgg cttcgctaat cacttttttg gtcatcgtct
     1741 ggatgagatg cagacgaaac tctttactgg cccgccatga agaacgcggg gcgacatctt
     1801 gcaggacaga ttcgctgata gcttccagcg tttgcaggtt taatggcgca ttttgtgcag
     1861 tctgttcggc atgttggcag cgaatcggcg ttggcgcggc aacaccaaat gccagacgta
     1921 attcgctgaa attgccgtta tccagtcggc aatgtgcggc gcagccaatc gttgaaatat
     1981 ccattgcgtc gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct ttcggctggg
     2041 gcggaaaatg aaaggcgaca aggatttcgt catgctcaag agacactttg cctggcccag
     2101 tgtgaaagcc attaatcggg acgaaacgaa caccgcgtgg ggagtggatc tccagctttg
     2161 cgtcataaat tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg caaatatttc
     2221 caccgtaggt agcgacgtta cggatctgcg gtccggcaat ggacgaggcc gcagcacata
     2281 acgccgggag atgacgttga gttatagaat cttctattag ctgggtaaat gtcgttgcag
     2341 agccgatacg tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc gccagattat
     2401 gaatatcaac aatatggcga taacggtcat tgtgatggtg gagctgaatc agtacgtcag
     2461 tgccaccggc gagcagtttg gcctgcgggt tgtcagccag caggttgatg gcatcggcaa
     2521 gggttgctgc gcgatggtaa gaagcaaaat caaacatgat gttatcctca aatcaatcct
     2581 gccagatgaa actcttcata taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca
     2641 ccggtagcca tcttcaccgc gttacgaata gctgcggcaa caggaattat tggtggctca
     2701 cccagtgact tatgtccgta tgcggattgc ggttcattga tttcgacgaa cgcgctttcc
     2761 agttgtggca gatccggcat ggtcggcatt ttgtaatcca gcagattggg gttacggacc
     2821 acgccgcttt tcgcatcgat gatcatctct tcaaatagcg cccagccaat gcccattccc
     2881 attccgccgt gtacctgacc ttctgccagc agcggattta gaatatgccc cgaatcatga
     2941 acgttgagga tgcggttgat ggtgactttg cacagtgcaa tatcgaccgt cagatcaaca
     3001 aaggtacagc caaacgccgg tgggttagtg gtggttttga tggagctttc agcagagagc
     3061 tgcccgccgc gttcagggtg gtagaaagcg tccatcgcca aatcttttaa cgacattaac
     3121 ggctcttccg gtcgttcaac cagcacgata tggcctttta tcagggtcag attcatcgct
     3181 gactgatgta gcatgactgc ggcgtgagcg atgattttct cttttaataa cagtgccgca
     3241 ctgcgcagcg caggcgcggc aacatagctc tggcgtgagg caaatgcgcc gggatcgaac
     3301 ggcgtaacgt cggtatcttg tgttgaaata acgcgaacgt cgctaaccgg aacccccacg
     3361 gtttctgcca ccatttgcga gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg
     3421 ctttgcacgt tgatggttcc atcctgattc atcagaaggc gcgcgcctgc tatttctacg
     3481 ccgacaggcc aggtattaga ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa
     3541 ttgccttgct ggttctggca ttctgcacgg cgtttttccc attcaaagat tttccggcct
     3601 ttttcaagac actccggcaa ccctgcgctg taaatacgtt tgcccgtgag cggattagca
     3661 tctccttcgc gggcggcgtt gcgtaaacga atttcaacag gatcaatacc taacgctgtc
     3721 gcggcgtcat caagcataga ctcaacggca aatacgactt gtggcgcgcc ataaccacgc
     3781 atcgcaccag ccgaggggag gttggtatag caggtctttg aactgtaagc gtaggcacaa
     3841 cgaggataaa ggtaagcgac tttattcccc cccgcagaag cgatggagtg cccgtgagat
     3901 gcataagcgc cggtgttaga cagaacatcc agactataac ctttcaatgt tccgtcgcgg
     3961 ctcacgccca tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac
     4021 tcttcacggc taagggaaac tttcaccgga atgccgccaa gcttgctggt caggaatgcc
     4081 gccattggct cttccagtac atcctgttta ttaccaaaac cgccaccgac aaatggtttg
     4141 atgactcgta cgcatgacca gggaatatcc agcgcctgac caaccacgcg gcgaacaatg
     4201 tgcgggatct gggtgctgga aacgatggta attcgcgagt catcctccat ccacgccagc
     4261 gatgttacgc tttccatgtg acaatgttga ataacggggg tctgatagtg cccctgtacc
     4321 tggtagtcgg cggcatcgat tgtttgttgg acattgcccg tcgacatcgt gctttgtttc
     4381 agtaaattgc cgccgttatg gattggtgca gcgtcctctg ccagcgccgc ttctggcgtg
     4441 gtgataacgg gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg
     4501 agttcatcgc gggccacgac gatggcaacg gcgtcaccat gatgacgaac atggcgagtt
     4561 agcagtgcgc gatcggcggt atcgcgcttg ttttcgtcaa gtgtccatgc atgcccagct
     4621 gtagcgaatg ggatatcagg cacatcttcc caggtaaaaa tcgccagtac gcctggtaaa
     4681 cttctggctt gttcatcatt aatacttacg gcataaccat gtgcgatagg gctacgtaca
     4741 tatttcgcat aacacatgcc cgccataaca taatcgtcag tatatcgtgc ccgcccggtg
     4801 accttagcaa tggcatcaac gcgcatgcat gattcacccg tagcggttgc ttcccgcgct
     4861 tccatagaaa tcccctcgat ttgaacattt ggttcttatg gcaaagattt agtctaagca
     4921 ggcaagattt acgccagaaa cgacgataag cgttagggga ggaaggaagt gtgatgacgt
     4981 taaaaaaaca cagatatgat acgaaacagg actctgaata ggggcagtga tacgtatcaa
     5041 tatgagtggc gataaaatca atgtgataaa ttgggattat tacgcatcag gatgggaaat
     5101 agggtaaaaa agtcaggaaa ttcgatacct ctacagcgaa aattttgctg atgatacttc
     5161 cctttgccaa atgaaattaa cctctttctc tattaccgcg tttttccaga agcaagagat
     5221 tgggtatcaa gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt
     5281 ttgagtgcgg gacgcctgaa taaaaaatct ctgggtatcg tgatgttgtt atcggttgga
     5341 ctgcttttgg cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc
     5401 gtttacaccg tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc
     5461 gtaaaagagc tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag
     5521 aaactaaaac tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca
     5581 acgaccaaaa ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca
     5641 gtagggcaac gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca
     5701 gcagatggcg gcaataaagg gattgatatc tctgctccac ggggtacacc tatttacgcc
     5761 gcgggtgcag gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc
     5821 atgattaaac acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta
     5881 aataatgggc aaagcgtgaa ggctgggcaa aaaatcgcca ccatggggag cacggatgcg
     5941 gcatctgttc gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt
     6001 tacttgccgc cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca
     6061 gcgcggcact tgctaaggag agcgtaaggt ttataatgcc ttacgcatct caaagcgggc
     6121 gtagttcaat ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc
     6181 ccgctccaat ttatcttcac ctcatcaacc tgccattttt gttgtatctg attttctata
     6241 ttatcttaat tcaatcggtt cagacttttc gcaatcgcag taacacttcc ccattttttt
     6301 gtaaataaaa agcgtttacg caatgcaat
//
LOCUS       DAZKFU020000254         6323 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZKFU020000254 DAZKFU020000000
VERSION     DAZKFU020000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.256"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(50..2083)
                     /gene="betT"
                     /locus_tag="ACHOQQ_RS20500"
                     /old_locus_tag="pgaptmp_004100"
     CDS             complement(50..2083)
                     /gene="betT"
                     /locus_tag="ACHOQQ_RS20500"
                     /old_locus_tag="pgaptmp_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGU4472288.1"
                     /translation="MTDLSHSRENDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2212..2799
                     /gene="betI"
                     /locus_tag="ACHOQQ_RS20505"
                     /old_locus_tag="pgaptmp_004101"
     CDS             2212..2799
                     /gene="betI"
                     /locus_tag="ACHOQQ_RS20505"
                     /old_locus_tag="pgaptmp_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGU4472289.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2813..4285
                     /gene="betB"
                     /locus_tag="ACHOQQ_RS20510"
                     /old_locus_tag="pgaptmp_004102"
     CDS             2813..4285
                     /gene="betB"
                     /locus_tag="ACHOQQ_RS20510"
                     /old_locus_tag="pgaptmp_004102"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGU4472290.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPVE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4299..5987
                     /gene="betA"
                     /locus_tag="ACHOQQ_RS20515"
                     /old_locus_tag="pgaptmp_004103"
     CDS             4299..5987
                     /gene="betA"
                     /locus_tag="ACHOQQ_RS20515"
                     /old_locus_tag="pgaptmp_004103"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGU4472291.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSTE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKDALPRSTAGYYVANGMPVRAKKMSRDLN"
BASE COUNT         1544 a         1758 c         1746 g         1275 t
ORIGIN      
        1 atgattcccg cgccatatgc agacatggcg cggttttatg caataacaat cacgcgtccg
       61 ggaacatcac gctatggccc ggcgcttcac gatggagatg aataaagtta aggtgccgct
      121 cgtactggtc aagaatatcg gtgatcacct gctctttgct gtagtccatc aggtcgttgc
      181 cctggctgcc ttctaacagg aaggtttcca gccggtagta ggtcgatttc ccgctacgtg
      241 cgcggtaggt aaagcccggc accgagtatt gctgcggcca aatctgatag acaaagtttt
      301 gctcttcgcc catatgcacc aacaaatcca gatgccccaa ctgttgtccc tcttccggcg
      361 gtaggctttt tagctccaca tacgcgccgc gcaaccgcag ctcttgcgcc acttcttcca
      421 ttgccgggta acagaccgtc tccatcatct gtttagtgta acgcgtgccc ggataattca
      481 tcaggcgcga gagacgtttt ttccagctca ggcgatcctg aagccccagc ggtcgcggtg
      541 cggtatcgcg gttggcactt tcacggcggt aatcttctac cttcagagat ttatacaacc
      601 ccgccatcac gaagaagatc acaaagctga acggcagccc cataatcacc gtggtgtttt
      661 gcagcgcgga tatcccgttg gtcatcagca tgccgagcgt cagcaggcca atcgccaccg
      721 accagaagac gcgcagccag ccgggggcgt cgctgttgat atctttaagc tgcgaggtga
      781 aattccccag caccagcgcc ccggagtccg ccgaggtcac ataaaacagc aggccagtaa
      841 tggtggcgac ggaggcgcta aaggtaaacg ccggatactg cgccagcagg ctgtagaagc
      901 cgcgctccgg atggaccatc gcttcctctg caaatgccgc gccgccgtgg atgatttcat
      961 acagcgcgct attgccgaac accgagagcc ataacagcgt gaaggtaaac ggaataatca
     1021 acgtgcccag cacgaactgg cgaatggtac gcccacgcga gatacgcgcc aggaacaagc
     1081 cgacaaacgg cgaccatgcc acccaccatg cccagaagaa gagcgtccag ttattcatcc
     1141 actcaactgg acggtcgaag gcaaaactgt tgagcgtcat gcccataaag cgattcacat
     1201 agtcgccaac attcagcacc agtgcattaa gcaggaacga agtgtcgccc ataaacaaca
     1261 cgaacaggat caatcccagc gccagcgcga cattaagctc cgataacacg cgaatgccct
     1321 tatcgacacc ggatgtcacc gagatagtgg cgattatcac cgacaaggcg atcagtgccg
     1381 cttttgccgc catcgaatcg ggaatatcaa acagtacgct caagccatag ttaagctgca
     1441 ccacaccgat accgagcgta gtggcaatac cgaagatagt gccgatcacc gctgcaatat
     1501 ccacagagtg acctatcggc ccgttaatcc gtttaccgaa gatcgggtac agcgccgagc
     1561 ggatggtgag cggcaaatta taacgatagc taaagtatcc gagcgccatg cccatcagcg
     1621 catacatcga ccagccggtt aagccgtagt gaaacagcgt ccagaccatc gcctgacgcg
     1681 cggcctcaat cgtctgtccc gcaccttccg gcggctgcat atactgcgtt accggttcgg
     1741 ctacggaaaa gaacatcaga tcgataccga tcccggcagc aaacagcatc gccgcccaac
     1801 tcagcaggct gaattccggt ttggattgtt ctggcccgag cttcaccgaa ccaaaacgcg
     1861 aacaagcgat acagaccaca aagacaatat agagcgttgc cgccagcaga tagtaccaac
     1921 caaaggtttt agaaacccag tccagcgtgc ggccaatcca cagggccgag aagtcgcgaa
     1981 acaggatcgt tgtcagggaa aacaacaaaa tcagtccggc ggaggtgtaa aacaccaccg
     2041 gattgatttt gtcattttcc ctgctgtgtg aaaggtctgt catccagtat ccccactgtt
     2101 attgttacca tttaaaatca aattcgtaac aattaagaca cattttatat tgaacgtcca
     2161 atcaataacc gctttaatag ataaacaacg ctgatgaatg gagtggcgaa aatgcccaaa
     2221 ttggggatgc agtcgatccg gcgcagacaa ctgatcgacg ccacactgga agcaataaat
     2281 gaagtgggca tgcacgatgc aacgatcgcg cagatcgccc gccgtgcagg cgtttctacg
     2341 gggatcatca gccactattt cagggacaaa aatggtctgc tggaagcaac catgcgcgat
     2401 atcaccagtc agctacgtga cgcggttttg aatcgattac atgcacttcc gcagggcagt
     2461 gcagagcagc gattacaggc gattgttggc ggaaacttcg atgaaacgca ggtgagcagt
     2521 gcggcgatga aagcctggct ggcgttctgg gccagcagta tgcatcagcc gatgctctat
     2581 cgtttacagc aggtcagcag ccgccgcttg ctgtcgaatc tggtgagcga gtttcgccgc
     2641 gaattgccgc gccaccaggc acaggaagcg ggctacggcc ttgccgcgct gattgacgga
     2701 ttatggctgc gcgcggcact gagcggcaaa ccgctggata aacctctcgc acactcgctg
     2761 acccgtcact ttattactca gcatttaccc accgattaac cgaggagacg tgatgtcccg
     2821 aatggcagaa cagcagcttt atatacatgg tggttatacc tccgccacca gcggtcgcac
     2881 tttcgagacc attaacccgg ccaacggtaa cgtgctggcg accgtgcagg ccgccgggcg
     2941 tgaggatgtc gatcgcgccg tgaagagtgc ccaacagggg caaaaaatct gggcggcgat
     3001 gaccgctatg gaacgctcgc gtattctgcg tcgggccgtt gatattctgc gtgaacgcaa
     3061 tgacgaactc gcaaaactgg aaaccctcga caccggaaaa gcatactcgg aaacctcaac
     3121 cgtcgatatc gttaccggtg cggacgtgct ggagtactac gccgggctga tcccggcgct
     3181 ggaaggcagc cagatcccgt tgcgtgagac gtcatttgtt tatacccgcc gcgaaccgct
     3241 gggcgtggtg gcggggattg gcgcatggaa ctacccgatc cagattgccc tgtggaaatc
     3301 cgccccggcg ctggcggcag gcaacgcaat gattttcaaa ccgagcgaag tcaccccgct
     3361 taccgcgtta aagctggctg aaatttacag cgaagcgggc ctgccggacg gcgtatttaa
     3421 cgtgttgccg ggcgtgggcg cggagaccgg gcaatatctg accgagcatc cgggcattgc
     3481 caaagtgtca tttaccggcg gtgtcgccag cggcaaaaaa gtgatggcta actcggcggc
     3541 ctcttccctg aaagaagtga ccatggaact gggcggtaaa tcaccgctga tcgttttcga
     3601 tgatgcggat ctcgatctcg ccgccgatat cgccatgatg gcaaacttct tcagctccgg
     3661 tcaggtgtgt accaatggca cccgcgtctt cgttccggcg aaatgcaaag ccgcatttga
     3721 acaaaagatt ctggcgcgcg ttgagcgcat tcgcgcgggc gacgttttcg atccgcaaac
     3781 taacttcggc ccgctggtca gcttcccgca tcgcgataac gtgctgcgct atatcgccaa
     3841 aggcaaagag gaaggcgcgc gcgtactgtg cggcggcgat gtactgaaag gcgatggcct
     3901 cgataacggc gcatgggtcg cgccgactgt gttcaccgat tgcagcgacg atatgaccat
     3961 cgtgcgtgaa gagatcttcg ggccagtgat gtccattctg acctacgagt cggaagacga
     4021 agtcattcgc cgcgctaacg ataccgacta cggcctggcg gcgggaatcg tgacggcgga
     4081 cctgaaccgc gcgcatcgcg tcattcatca gctggaagcg ggtatttgct ggatcaacac
     4141 ctggggcgaa tccccggtag agatgcccgt tggcggctac aaacactccg gcattggtcg
     4201 cgagaacggc gtgatgacgc tccagagtta cacccaggtg aagtccatcc aggttgagat
     4261 ggctaaattc cagtccatat tctaaccagg aggtttattt gcaatttgac tacatcatta
     4321 ttggtgccgg ctcagccggc aacgttctcg ctacccgtct gactgaagat ccgaatacct
     4381 ccgtgctgct gcttgaagcg ggcggcccgg actatcgctt tgacttccgc acccagatgc
     4441 ccgctgccct ggcattcccg ctacagggta aacgctacaa ctgggcttac gagacggaac
     4501 ctgaaccgtt tatgaataac cgtcgcatgg agtgcggacg cggtaaaggc ctgggtggat
     4561 cgtcgctgat caacggcatg tgctacatcc gtggcaacgc gatggatctc gacaactggg
     4621 caaaagaacc cggtctggag aactggagtt atctcgattg cctgccctac taccgcaagg
     4681 ccgagacccg cgacgtgggc gagaacgact accacggcgg cgacggcccg gtgagcgtca
     4741 ccacctccaa acccggcgtc aatccgctgt ttgaagcgat gattgaagcg ggcgtgcagg
     4801 cgggctaccc gcgcacggac gatctcaacg gctatcagca ggaaggtttt ggcccgatgg
     4861 atcgcaccgt gacgccgcag ggccgtcgcg ccagcaccgc gcgcggctat ctcgatcagg
     4921 ccaaatcgcg cccgaacctg accattcgta ctcacgctat gaccgatcac atcatttttg
     4981 acggcaaacg cgcggtaggc gtcgagtggc tggaaggcga tagcaccatt ccaacccgcg
     5041 cgacagcgaa taaagaggtg ctgttatgtg caggtgcgat cgcctctccg cagatcctgc
     5101 aacgttccgg cgtgggtagc actgaactgc tggcggagtt tgatattccg ctggtgcatg
     5161 atttacccgg cgtcggcgaa aatcttcagg atcacctgga gatgtatctg caatatgagt
     5221 gcaaagaacc ggtttccctc taccctgccc tgcagtggtg gaatcagccg aaaatcggtg
     5281 cggagtggct gtttggcggc accggcgttg gtgccagcaa ccactttgaa gcaggcggat
     5341 ttattcgcag ccgagaggaa tttgcgtggc cgaatattca gtaccatttc ctgccagtag
     5401 cgattaacta taacggctcg aatgcagtga aagagcacgg cttccagtgc cacgtcggat
     5461 cgatgcgctc gccaagccgt gggcatgtgc ggattaaatc ccgcgacccg caccagcatc
     5521 cggcgattct gtttaactac atgtcgcacg agcaggactg gcaggagttc cgcgacgcaa
     5581 ttcgcatcac ccgcgagatc atgcatcaac cggcgctgga tcagtatcgt ggccgcgaaa
     5641 tcagccccgg cacggaatgt cagacggatg agcagctcga tgagttcgtg cgtaatcacg
     5701 ccgaaaccgc cttccacccg tgcggtacct gcaaaatggg ttatgacgag atggcagtgg
     5761 ttgacggcga aggccgcgtg catgggctgg aaggcctgcg cgtggtggat gcgtcaatta
     5821 tgccgcagat tatcaccggc aatttgaacg ccacgacgat tatgattggc gagaaaatag
     5881 cggatatgat tcgcgggaag gatgcattgc cgaggagcac ggcgggatat tatgtggcaa
     5941 atgggatgcc ggtgagagcg aaaaaaatga gtcgtgattt gaactgactc aggaaccgat
     6001 cagattgaga gttaccgttc caggtatggg gacagaatcg ttatataaaa cataggggta
     6061 actctcatgc ttcatagtga caacctcgcc agcaaattct aagctgactt gctcaatctg
     6121 gctgatgaag gtgctatgca tacacagata aactacagat gaaataccac tcagcttaag
     6181 aattacgccg ctaatgttcc ttgctatggt tacctgttca ttcccgctca cggttagcat
     6241 gtacgaaaag ggttcttgta acgagtacaa aaataagtca gaccaaaacc acatggagct
     6301 ttaaacacgg ttcgtataat tta
//
LOCUS       DAZKFU020000255         6303 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZKFU020000255 DAZKFU020000000
VERSION     DAZKFU020000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.257"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..129
                     /locus_tag="ACHOQQ_RS20520"
                     /old_locus_tag="pgaptmp_004104"
     CDS             <1..129
                     /locus_tag="ACHOQQ_RS20520"
                     /old_locus_tag="pgaptmp_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4472292.1"
                     /translation="EMLHRFFALYADVHLFNQLTLVLQPTGKRLRWKENHNLHVPG"
     gene            93..1181
                     /gene="tssG"
                     /locus_tag="ACHOQQ_RS20525"
                     /old_locus_tag="pgaptmp_004105"
     CDS             93..1181
                     /gene="tssG"
                     /locus_tag="ACHOQQ_RS20525"
                     /old_locus_tag="pgaptmp_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGU4472293.1"
                     /translation="MERESQPARTGLTQALALNNDIWRASFYRFCQLLEQENPDGPKL
                     GTTSHPGDDPVRFRPWPGMGFPVSTLKAVEIDEDRPTLPPTIRTTFLGMYGVDSPLPT
                     SWLDDITQRREGHEALTSFLDIFSHRITTQYYRIWRKYAYPATFEEGGRDATSQCLLG
                     LVGLGIPGTAEQVATPVSRFLALLGAMRLPTRNAEGIRALVSLLAPDTRALITEPDPV
                     KVHIGNRSGLGAGNRIRLSQRAPLGKTTGEACSRLLMTLETADPDEAEGWLPGGVLHT
                     DLLVLLRVYLGYRSDVRLRLTVPVRLLPEPRLGKGRPVQLGRTGLLGLKAGKLSNGRE
                     SLTVSLGCYEGLKCAALPSAEDGHYRFE"
     gene            1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHOQQ_RS20530"
                     /old_locus_tag="pgaptmp_004106"
     CDS             1215..1739
                     /gene="tssJ"
                     /locus_tag="ACHOQQ_RS20530"
                     /old_locus_tag="pgaptmp_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU4472294.1"
                     /translation="MVTVIRWWAPMFIACGLLAGCGLTQTVTDGMISITKSIFYKKIK
                     TLHLDFTPRTAINTDGAQTPLATMVRVYQLRDRKAVDAAGYQMLLRRADTVLKDDVLA
                     SKELLVMPGGSVTLNMPMDEDAQFVAVVGLFNRPDQKDNRWRLVLTRDDLNPDKPRTI
                     ELGDGWLSLVPVKE"
     gene            1744..4296
                     /locus_tag="ACHOQQ_RS20535"
                     /old_locus_tag="pgaptmp_004107"
     CDS             1744..4296
                     /locus_tag="ACHOQQ_RS20535"
                     /old_locus_tag="pgaptmp_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGU4472295.1"
                     /translation="MDNRSAFFDGQPRYFLEINGSAVKPDVLRFRGREALSEPFRWDI
                     GFTSRNANIPPEEVLMKYASLRMRNGKNVHGIVTRLEWLSTSKDQSHYRLTLSSRLAL
                     LEYTRQCAVFQNQSVPEVVEQVLRKHGLEGADFEFRLERAYPSREIITQWQETDLQFI
                     QRILSEVGIFWRTEMDDVRGLDTYIFADSQLNYQFDVCLPYSEPSGLFDGAAESVWDV
                     RTWHNVAIGTVSTRDYNYRTASMPMDATVSVRNDAVTTGEHYRDAPAYREAGDDTSPE
                     PETESGAFYARLHHERELNRSARIHLFSNAAHLSPGQVLEPQGDVIADLKEGIVLTLV
                     TYRGARDSRLHVSAWGMPYTERYCFRPAEIPRPVIPGTLPARIESRGKNDIYAHLDGQ
                     GRYRVKMDFDREETEPGFGYLWLRMAKPCAGETLGWHTPLIDGTEVAIAYSNGDIDLP
                     YIAYALHDAEHPDLVSRDNHTRNILRTPANNKLRMEDRRNEEHIKLATEYGKTQLNSG
                     HLVDSQGQLRGKGTELRTDEWGTLRAGKGLFVAADAQAKAQGEALDMDAALKEIDRLN
                     QQLQQLENAAERAQALKADIDSQIQMFEQRLKPLNEAVLFSAPEGIALTSGEDVQLAA
                     GQNLAVNAGGDISTGTMGNLTALAGERLGLFARSGQLSVKSGEGPVEVQAQNGSMRLF
                     AEKKLTLSSASDISFAGKKRITLIGGGSYLRLEAGKIEYGTTVSYLRKVKRTIAAGAA
                     SQQPEMPWLADPVKPSASALMQYLYHDDVPVVGAPFKATLADGTVREGNLDGAGYLHL
                     EDVPPGRVLVELGPDTRDYLRKNKTDNRLFVGTSLSEADIDSLIAKYSEGVE"
     gene            4293..5837
                     /locus_tag="ACHOQQ_RS20540"
                     /old_locus_tag="pgaptmp_004108"
     CDS             4293..5837
                     /locus_tag="ACHOQQ_RS20540"
                     /old_locus_tag="pgaptmp_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472296.1"
                     /translation="MSGVNVAWFKETSLETGQWIWGTLQGAFNEKQTIGQIIVDAVIG
                     MIPLVGDVTAVRDLIAVSIGLAKEPKKRESVTEWVFLVILLFALIPVIGGVIKGVGRL
                     ALRVTTKVAENVETLGAVIKFLNRIGEGDAVKWLNKLNVVEYQAQLIEKMGDFCNTMI
                     TAINKILKARVGRMLPEKWQADLLLVRDGFAALQRLASRMIPQALKELDAKLRVLQAL
                     VYKGEIHTITTGMGRAEVRREAEAYLIERPLCEAPKQGTRYPSVHAEVIHEDRLRLKY
                     SREGYPDLLGYKGKSPAFGDEQVFTNIASFSGEITAKSASEMGGSTIFRAFGNPTELA
                     PNGSWAAGGFWGYQKVPGSAEEWRQLSAVLDEWNGNGFLVVLHLPEDLATRMPGSNAW
                     HGKIAEQFGQKRPDQYLQGGGEQVIINLDETLKTELNRIGEQVKASGQAVSIEINGVN
                     AEFYPTGWKEVEGVHGYSKPEGIEPPYVTYTRELHDDELRSKVTERTILNAARIEYQT
                     TTGSEQ"
     gene            5834..>6303
                     /locus_tag="ACHOQQ_RS20545"
                     /old_locus_tag="pgaptmp_004109"
     CDS             5834..>6303
                     /locus_tag="ACHOQQ_RS20545"
                     /old_locus_tag="pgaptmp_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472297.1"
                     /translation="MITIEKTKYPMSDEQVRRKLFWVLQRLSSYSLWQRKRDAWAYFT
                     QQYEQALKNWPEEITKGFDPEAIIWAYDALRLYDEGLSELAVGNRQVWQRLTGEFHQL
                     ARPVDLIESYFYLPCHERGVQQEPYPVEVEKLNKLKIAAKIHGDYLMYPPHNNV"
BASE COUNT         1503 a         1516 c         1945 g         1339 t
ORIGIN      
        1 gagatgctgc accgtttctt tgcgctctac gctgatgtcc accttttcaa ccagctcaca
       61 ctggtactgc aacccacagg gaaacgactg agatggaaag agaatcacaa cctgcacgta
      121 ccgggctgac gcaggccctg gcccttaaca acgatatctg gcgggccagt ttttatcgtt
      181 tctgccagct gctggagcag gaaaatccgg acgggccaaa actgggcacg accagccatc
      241 cgggcgatga tccggtgcga ttcaggccct ggcccgggat gggattccct gtcagcaccc
      301 tgaaagcggt ggagatagac gaagaccgtc cgacattacc cccaacaatc cgcacgacat
      361 ttctcggaat gtatggggtg gactcacctc tgccgacgtc ctggctggat gatattacgc
      421 agcgccgtga aggacatgag gcgctcacct catttctgga tattttcagc caccgcatca
      481 ccacgcagta ctaccggatc tggcgaaaat atgcgtatcc ggccactttt gaggaggggg
      541 gacgcgatgc cacatcgcag tgtctgctgg ggctggtcgg actcggtata cccggcaccg
      601 ctgaacaggt ggcaacgccg gtttcccgtt ttctggcctt actgggtgcc atgcgattac
      661 ccacccgcaa tgcggaaggc atccgggctc tggtgagtct gctggcaccg gatacccgcg
      721 cacttatcac tgaacccgat ccggttaagg tccatatcgg taaccgcagt ggactgggtg
      781 ccggaaaccg catccgtctt tcgcagcggg cgccgctggg taaaacgaca ggggaagcat
      841 gcagccggct cctgatgaca ctggaaacag cagatccgga cgaggcggaa ggctggttgc
      901 ccggaggtgt tctccatacc gatttgctgg tcctgctgcg cgtttatctc ggctaccgca
      961 gtgacgtccg gctgcgcctt actgtccccg tgcggttgtt gcctgagccc cgcctgggca
     1021 aaggcaggcc tgttcagctg ggccgtaccg gtttactggg gctgaaagcg ggcaaactca
     1081 gcaatggccg ggagagtctg acggtcagtc tgggttgcta cgaagggctg aaatgtgctg
     1141 cgctaccgtc tgctgaagac gggcattatc gcttcgaata atcacctgtt ctctctcaat
     1201 aaaggaacgc ctgaatggta acagtaatcc gctggtgggc gccgatgttt attgcctgtg
     1261 gcctgctcgc aggctgcggt ctgacgcaga ccgtcacgga tggtatgatc agcatcacga
     1321 aatccatctt ctacaaaaaa atcaaaacgc tgcatctgga ttttaccccg cgtacggcca
     1381 ttaacactga tggggcacag acgccactgg caaccatggt gcgggtttat cagctcaggg
     1441 atcgtaaagc ggttgatgcc gccggttacc agatgctgct gcgccgggcc gatacggtac
     1501 ttaaggacga cgtgctggcc tcaaaagaac tgctggtgat gcccggcggc agcgtgacgc
     1561 tcaatatgcc catggatgaa gatgcgcagt ttgtggctgt ggtgggactg tttaaccggc
     1621 cggatcagaa ggacaatcgc tggcgcctgg tactgacccg tgatgacctc aatccggata
     1681 agccccgcac cattgaactg ggtgatggct ggctcagtct tgtgccggta aaggagtgac
     1741 gtgatggata accggtcagc gttttttgac ggacagcccc gttactttct ggaaattaat
     1801 ggcagtgcag taaagcctga tgttttacgt tttcgtggca gggaagcact gagtgagccg
     1861 ttccgatggg acatcgggtt cacctcccgg aatgcgaaca ttcctccgga agaggtgctg
     1921 atgaaatatg cgtcgttgcg gatgcgtaac ggcaaaaatg tccatggcat tgtgacccgt
     1981 ctggaatggc tgtccacatc gaaagaccag tcccactacc ggcttacact cagctcccgt
     2041 ctggcactgc tggagtacac ccggcagtgt gccgtatttc agaaccagtc cgtccctgag
     2101 gtggtggagc aggtgctgcg caaacacggg ctggagggcg ctgattttga attcagactg
     2161 gaacgcgctt acccgtcgcg tgaaattatc acccagtggc aggaaacgga tttgcagttc
     2221 attcagcgca ttctgtctga ggtggggatt ttctggcgca cggagatgga tgatgtgcgc
     2281 ggactggata cgtacatttt tgccgacagc cagcttaact accagtttga tgtgtgtctg
     2341 ccgtacagcg agccgtcggg cctttttgac ggtgctgcag aatcggtctg ggatgtacgg
     2401 acatggcaca acgtcgccat cggcacggtc tccacgcgtg attacaacta ccggactgcc
     2461 tccatgccga tggatgcgac ggtcagcgtg cgtaatgatg cggtcaccac cggggagcat
     2521 taccgcgatg caccggctta tcgtgaagcg ggggatgaca ccagcccgga gccggaaacc
     2581 gaatctggtg cgttttacgc ccggcttcac cacgagcggg aactcaacag gtcggcccgc
     2641 attcacctgt tcagcaacgc cgcacacctc tcccccgggc aggtgctgga gccgcagggg
     2701 gatgtcattg cggacctgaa ggaagggatt gttctcacac tggtgactta ccggggggcc
     2761 cgggattccc ggctgcatgt gtcagcatgg ggaatgcctt acaccgaacg ttactgtttc
     2821 cgtccggcag aaatcccgcg tccggtcatc ccgggtacgc ttccggcacg gattgagagt
     2881 cgcgggaaga acgatatcta tgcccatctg gacgggcagg ggcgttaccg ggtgaagatg
     2941 gactttgacc gggaggagac ggaacccgga ttcggctacc tgtggctgcg gatggcaaaa
     3001 ccctgtgcag gtgaaacgct gggctggcat acgccgctca ttgatggcac cgaagtggcg
     3061 attgcataca gcaatggcga tattgacctg ccctacatcg cgtatgcact gcacgacgct
     3121 gagcatcctg acctcgtgag ccgggacaat cacacccgga acatcctcag aacgcctgcg
     3181 aacaacaagc tgcggatgga agacagacgc aatgaggagc acatcaaact cgctacggaa
     3241 tacggcaaga cacagcttaa cagcggacat ctggtggaca gccaggggca actgcggggt
     3301 aaaggcacag agctgcgtac cgatgagtgg ggaaccctga gagcagggaa gggacttttt
     3361 gttgctgcgg atgcgcaggc gaaagcacag ggcgaggcac tggacatgga tgccgcgctg
     3421 aaggaaatcg acaggcttaa tcagcaactg cagcagctgg aaaatgccgc agaacgggca
     3481 caggcgctga aggctgatat tgacagtcag atacagatgt ttgagcagcg gctgaaaccg
     3541 ctcaatgaag ccgtgctttt ctcggcgccg gaaggcattg ccctgaccag cggtgaagat
     3601 gtgcagctgg ctgctggtca gaacctggct gtcaacgccg gaggtgatat cagtaccgga
     3661 acaatgggca acctgaccgc gctggccgga gagaggctgg ggctttttgc caggagcgga
     3721 caactgagcg tcaaatccgg ggaggggcca gtagaggtac aggcgcaaaa cggcagcatg
     3781 cggttgtttg ctgagaagaa gctgacgctg agttcggcaa gtgatatctc ctttgccggg
     3841 aaaaaacgta tcacgctgat tggcgggggg agttacctgc ggctggaggc ggggaaaata
     3901 gagtacggta cgacggtgtc gtatctgcgc aaagttaaac ggactatagc ggcgggggct
     3961 gcgtcacagc agccggaaat gccttggctt gccgacccgg ttaaaccctc tgccagtgcg
     4021 ctgatgcagt atctgtatca cgacgatgtc ccggtagtgg gcgcgccttt caaagcgact
     4081 cttgctgatg gcactgtacg ggaaggtaac cttgacggtg cgggatatct gcatctggaa
     4141 gacgtcccgc cagggagagt gctggtggag ctcgggccgg ataccagaga ttatttacgt
     4201 aaaaataaga ccgataatcg gttgtttgtt gggacatctc tcagcgaggc tgacattgac
     4261 tcactgatag cgaagtacag cgaaggtgtg gaatgagtgg agtaaacgtt gcctggttta
     4321 aggaaaccag cctggaaacc ggacagtgga tctgggggac gcttcagggg gcatttaatg
     4381 aaaaacagac cattgggcag atcatcgttg atgcggtgat cggcatgatc ccgctggtcg
     4441 gcgatgtaac ggcagtgcga gatctgatag cggtttcgat aggactggca aaagaaccga
     4501 agaaacggga aagtgtgact gaatgggtgt ttctggtgat cctgctgttt gcgctaatcc
     4561 cggtaattgg tggcgtgata aaaggtgtcg gacgtctggc cctgcgtgtg acaacaaaag
     4621 tggcagagaa tgtggaaacc cttggcgctg tgattaagtt tctgaaccgt atcggcgaag
     4681 gtgatgcggt gaagtggctg aataagctga atgtcgttga gtatcaggca cagttgattg
     4741 aaaagatggg ggatttttgc aacacgatga ttaccgccat caataaaatc ctgaaagccc
     4801 gggtcgggcg gatgttgccg gagaaatggc aggcggattt actcttggtt cgggatggtt
     4861 ttgcggcact gcaacggctg gcgtcgagaa tgatcccgca ggcgttgaaa gagctggatg
     4921 cgaaactgcg ggtgttgcag gcgctggttt ataaaggtga aatacataca atcacgacgg
     4981 ggatgggacg tgctgaagtg cgccgtgagg ctgaagcata tcttattgag cgtccgctgt
     5041 gtgaagcgcc gaagcagggg acgcggtatc cgagtgttca tgcggaggta attcacgaag
     5101 acagattgcg gcttaaatat tccagggagg gatatccaga tttgttggga tataaaggaa
     5161 aaagtccggc atttggtgat gagcaagtat ttacaaatat tgcctctttt agcggcgaga
     5221 ttaccgcaaa aagtgcaagt gagatgggcg gtagtacgat ttttcgcgct tttggtaatc
     5281 caacagaatt agcacccaac ggttcttggg cggctggcgg attctggggg tatcaaaaag
     5341 tgccgggcag tgctgaagaa tggcgtcagc tttcagctgt actggatgag tggaatggta
     5401 acggttttct ggtggtgctg catttgccag aagacctggc aactcgtatg ccgggtagca
     5461 atgcttggca cgggaaaatt gctgaacagt tcggacagaa gagacctgac cagtatctgc
     5521 aaggtggtgg cgaacaggta attattaatc tggatgaaac gttgaaaaca gagttgaacc
     5581 gcatcgggga gcaggtaaaa gccagcggtc aggctgtcag tattgaaatt aatggcgtaa
     5641 atgccgagtt ttatccgacg ggctggaaag aggtggaagg cgtgcatggt tacagtaagc
     5701 cggaagggat agaaccgccg tatgtgacgt atacccgtga actgcatgac gatgaactgc
     5761 gcagcaaagt gacagagcga acgatactta atgcggcccg cattgagtac cagaccacga
     5821 caggaagcga acaatgatta ccattgaaaa aacaaaatac ccgatgagcg atgagcaggt
     5881 acgtcgcaaa ttattctggg tgttacagcg gctgagcagc tatagccttt ggcagcgcaa
     5941 acgggatgcc tgggcctatt ttacccagca gtatgaacag gcgctgaaaa actggccgga
     6001 agagataacg aaggggtttg atcccgaggc gataatctgg gcatatgatg ctttgcgtct
     6061 ttacgatgaa gggctatcag aattggcagt gggaaatcgg caggtatggc agcgtttaac
     6121 gggagaattt catcaacttg ctcgcccagt ggatttaatt gaatcttatt tttatctccc
     6181 ctgccatgaa cgaggtgtac agcaagaacc atacccggta gaagtagaaa agcttaataa
     6241 gctgaagatt gcagcaaaaa tacatggtga ctatctaatg tatccaccac ataacaatgt
     6301 atg
//
LOCUS       DAZKFU020000256         6260 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZKFU020000256 DAZKFU020000000
VERSION     DAZKFU020000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.258"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1351
                     /gene="paoC"
                     /locus_tag="ACHOQQ_RS20550"
                     /old_locus_tag="pgaptmp_004110"
     CDS             <1..1351
                     /gene="paoC"
                     /locus_tag="ACHOQQ_RS20550"
                     /old_locus_tag="pgaptmp_004110"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGU4472298.1"
                     /translation="ATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLY
                     AGANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDT
                     QVDPADPTRRFSRRQLIECLRTGANKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNL
                     LEKSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFP
                     VSAGSGGQWGANTSTSGVYAACMKLREMIASTVGFDPEQSQFADGKITNGTRSATLHE
                     ATAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRIL
                     NPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFL
                     DDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            1361..2317
                     /gene="paoD"
                     /locus_tag="ACHOQQ_RS20555"
                     /old_locus_tag="pgaptmp_004111"
     CDS             1361..2317
                     /gene="paoD"
                     /locus_tag="ACHOQQ_RS20555"
                     /old_locus_tag="pgaptmp_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HGU4472299.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCEREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLADVASVRLHQEEDSYLPPSS"
     gene            complement(2495..3622)
                     /locus_tag="ACHOQQ_RS20560"
                     /old_locus_tag="pgaptmp_004112"
     CDS             complement(2495..3622)
                     /locus_tag="ACHOQQ_RS20560"
                     /old_locus_tag="pgaptmp_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4472300.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSTFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(3764..4822)
                     /locus_tag="ACHOQQ_RS20565"
                     /old_locus_tag="pgaptmp_004113"
     CDS             complement(3764..4822)
                     /locus_tag="ACHOQQ_RS20565"
                     /old_locus_tag="pgaptmp_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4472301.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDVAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGKDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLRYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            5087..5971
                     /locus_tag="ACHOQQ_RS20570"
                     /old_locus_tag="pgaptmp_004114"
     CDS             5087..5971
                     /locus_tag="ACHOQQ_RS20570"
                     /old_locus_tag="pgaptmp_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4472302.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
BASE COUNT         1468 a         1744 c         1728 g         1320 t
ORIGIN      
        1 cgccaccctt cagcacttgc gtatcggtgc cgaccagagc gggaaaatca ccgctatctc
       61 acatgaaagc tggtccggaa acctgcccgg cggcacgccg gaaacggcgg tacagcaaag
      121 cgaattactc tacgccgggg caaaccgtca taccggcctg cggctcgcca cgcttgattt
      181 gccggaaggg aacgccatgc gtgcgcccgg cgaagccccc ggtctgatgg cgctcgaaat
      241 cgcgatcgac gaactggcgg aaaaagcggg catcgatccc gtcgagtttc gcatcctgaa
      301 tgacactcag gttgaccccg ccgacccgac gcgccgcttc tctcgccgtc agcttatcga
      361 gtgcttgcgc accggagcga ataaatttgg ctggaagcag cgcaacgcca cacccggaca
      421 ggtgcgcgac ggggagtggc tggtcggcca cggtgttgcg gcgggctttc gcaataatct
      481 gctggaaaaa tcgggtgctc gggttcacct cgaacaaaac ggcaccgtta ccgtagaaac
      541 ggacatgacc gacattggca ccggcagcta caccattctg gcccagacgg cagcggaaat
      601 gcttggcgta ccgctggagc aggttgcggt tcacctcggc gattccagtt tcccggtttc
      661 tgcgggttct ggtggacaat ggggcgcgaa tacctccacc tccggcgttt acgccgcctg
      721 tatgaagctt cgcgaaatga ttgcctcgac agtcgggttt gatcctgagc agtcgcagtt
      781 tgccgacggc aagattacca acggtacccg aagcgccacg ctacatgagg ccaccgcagg
      841 cggcagactg acagcggaag agagcattga attcggaaca ctgagcaagg agtaccagca
      901 gtcgaccttt gccgggcatt ttgtggaggt cggcgtgcat agcgcgacgg gagaagttcg
      961 ggtccggcgt atgctcgctg tgtgtgctgc aggacgcatc ctgaatccga aaactgcacg
     1021 cagccaggtc attggcgcaa tgactatggg catgggcgcg gcactgatgg aggagctggc
     1081 ggtggatgac cgtttgggct acttcgttaa tcacgatatg gcggggtatg aggtgccggt
     1141 ccatgcggat atcccaaaac aggaggtgat tttcctggat gataccgacc ccatatcctc
     1201 cccgatgaag gccaaaggtg tcggtgagct gggcctgtgc ggcgtgagcg cggctatcgc
     1261 caacgcggtg tataacgcca ccggtattcg ggtacgggat tatcccatca ctctggataa
     1321 gctgctcgat aagctgccgg atgtggttta aggaggaaca atgtcatacc cgctttttga
     1381 caaagacgaa cactggcata agccagagca ggcgtttctc accgatgacc accggaccat
     1441 tctgcgcttc gccgtagagg cgctaatgtc cggtaaagga gcggtgctgg tgacgctggt
     1501 ggagatacgc ggcggcgcgg cgcgcccgct cggggcgcag atggtggtgc gcgaagatgg
     1561 tcgttactgc ggttttgtct ctggcggctg cgtggaggcc gctgccgctt ttgaagcgct
     1621 ggagatgatg ggctcaggct gcgaacgcga aattcgctat ggcgaaggtt cgccgtggtt
     1681 tgacatcgtt ctgccctgcg gcggtgggat cacgctgacg ctccataaac tacgctcggc
     1741 acagcctctg ctcgccgtgc tgaaccgcct ggaacagaga aaaccggcgg ggctgcgcta
     1801 cgatccgcaa gcacaatcgt tggtgtgcct gcccacgcaa acccgaacgg gctggaatct
     1861 caatggcttt gaggtggggt tcaggccatg cgtcaggctg atgatttacg gacgttctct
     1921 tgaggcgcag gcaaccgcga gtcttgcagc agccacaggc tatgacagcc atatcttcga
     1981 tctttttccg gcctcagcca gcgctcagat cgataccgat acggcggtca ttttgctgtg
     2041 ccatgatctc aaccgagagc tgccagtgtt gcaggccgcg cgagaggcaa aaccctttta
     2101 tctcggcgca ttgggcagct atcgaaccca cacgttacgt ctgcaaaagc tccacgagct
     2161 gggatggtcc agggaggaga cagcgcaaat ccgggcaccc gtcgggatat ttcccaaagc
     2221 ccgggatgcg catactctgg cactctccgt gctggcagac gtcgcctctg tacgtctcca
     2281 tcaggaggag gattcatacc tgcccccgtc gtcctgatcc ttgcggccgg gcgtgggaag
     2341 cgctttctca cctccgggga aataccataa attaagagaa aaatctgcag tagccgtttt
     2401 agaaaatcac ctaaatcgtt taaaagagcg ccctacccat agtgggtgct catcgccttg
     2461 tgtaacttat tcagcaacgc gcgccctcgc cggactaaag cgcaccggcc ttctggcgtg
     2521 cggtaacaaa ggtcagtacg cccgcgcaaa ggagcagcac actactcatg gcaaacgtac
     2581 tctgccagcc aaggcggtca aacacgatgc ctcctgccgt tgatccaagg gcgattgaga
     2641 gctgaatcac cgcgaccatg agaccgcctc cggcttcggc gttgtcaggc aacgtacgcg
     2701 caatccatgt ccaccatccg gttggcgcgg cggtggccag catgccccag agtcctaaca
     2761 gcagcgagac cgtccagacg tgatgcccgg taagcatcag accgatggca atgacggcca
     2821 tcagtccggg tatggccatc agcgtggggt agaattttct gttgagaaat gtcgaaacaa
     2881 ccagcgtgcc aataaaaccg gccacgccga taatcagcaa aatcaacgac aagcctaagg
     2941 aattaacccg tgtcaccgac tccagaaacg ggcgcacata ggtgaatagc gcaaactgcc
     3001 ccatgaaaaa caggccacaa gctatcatgc cgaccacaac catgcggcgg ctaaataagc
     3061 ggaataccgc gccacatgac gtgtggcttt tattcgcatc catgctgggc agactaatgc
     3121 actgccagat aaaggcgaca accgccatgg gcaccaggca caagaaagcg cctcgccatc
     3181 cgaccgtagc ccccagatag cttcccagcg gcgcggccac gaccgtcgcc agcgcattac
     3241 cggcgttgaa aatcgccagc gcacgcgtga cctgatgctg cggcaccaga cgaatcgccg
     3301 ttgccgctga catagaccag aatccgccaa tggccacacc aatcatcgca cgaccgacca
     3361 tatacatcag ataactggta gccagcgcaa taataagccc cgatacggcc atcagaaccg
     3421 tcatccccag cagcaggaac ttacgattca tctttccagc tagcgttgaa agcgtcaggc
     3481 tggtcagaac agccagcgcg ccggagatag caatcccctg ccctgcaagc ccctccgtca
     3541 cgcctaaatc gcgggtaatc ggcgtgagca ggctgacggg cataaattca gaagcaatca
     3601 gcacaaacac gcacagggtc atagcaaaaa taccgcccca gtatgcgtgc tgatgatgag
     3661 gtgatggtgt ttggcttagt gtcgacatag tgaaatttca caaagatcag gataaaagcc
     3721 acctgccata gaccggtggc tttgcatcaa ttcagaagag gttttatttc agtttggtct
     3781 ggaaaaattg ttcgaacttc gcgaaaggta tttttccggc cacgttatcg tacaggtcca
     3841 cgtgatttgc ccctggtacc accaccaact ctttgtcttt actgccaacc gccttgtaag
     3901 catcttcagc aaaatagcgt gaatgcgctt tctcaccggt cacaatcagc gtcgggatgg
     3961 tgatttcact ggcgtatctc aacagcggca tattcataaa tgacagcggc atcgttgccg
     4021 tccacgcccc ggtagagtta acggaacgtt cgtggaagcc gcgcggcatg cgatagtagt
     4081 caaagaactc tttcagtacc ggatgaggat ttgccggcag cgtttctggc agaatgcgct
     4141 cgcccgcact gaccttgccg ttgctgtcga cattaatatc atgaccaccg tgagcaaacg
     4201 ttccgctctg cgcatccttc cagcgctgtt cattcagata ttgcagaaca gcacgacggt
     4261 cggcggtaga ataacggtct ttgccatccc ccacaccatg acccatcgcc cggctcatgt
     4321 catacatcac gctggtagct accgctttaa cgcgggtatc catcgccacg tcgttcagcg
     4381 ccatgccgcc ccagccacaa ataccgagca gcccgatacg gttgcggtct acctcttttt
     4441 gcagccctaa gaaatccacc gccgcgctga aatcttcagt gttgatatcc ggtgaggcga
     4501 cgtttcgcgg atagccgccg ctttccccgg tgtaagaagg atcgaacgcc agggtaacaa
     4561 accctcgttc cgccagcgtc tgcgcataca ggccgctgga ttgctctttc accgcgccaa
     4621 atggcccact gaccgcaatc gccgccagct tgcgatcgcc acggtcttta ggcaggtaaa
     4681 gatcgcccac taaggtgatg ccatatcggt tcgggaacga gactttacgg tgatcgactt
     4741 tctggctctc ggcgaatgtt ttatcccatt tatctgtcat ggacacaggg gcattaggat
     4801 tggttgaatc agcataactc attgtcgtga ctccacttaa tgatgcgaaa agcagcatgg
     4861 tcggcatcgc ggtttttagc gttcgggtga aggctttcat aaaaatcccc ataaaaaaca
     4921 acagagagat acaggtttcc gacggacaac gctgcggtgc tttactttga tggggtcatt
     4981 atagtgagca gaattagtgc tgattagggg gcgattaatg ctaagattaa taccatattg
     5041 ttataaatta atgcggagat tgccggcatg aaggggggat gtttcaatgg cgaaaaggga
     5101 gaactacaac gagctgtacc tgtttatgca ggtggtgcgg gaaggcagct ttaccgcagc
     5161 ggctcagcgg ctgggtcttg ctcagtcagg agtcagccgt tctgttcgcg agcttgaaga
     5221 aaggctgggt gtccagctgc tagtgcgcac cacgcgtaaa ctgtcgctga cacaagcagg
     5281 cgagcagctc taccagaaga cggcatctgg atttgaaatg ttagatcttg ggcttgccac
     5341 actggcgcac tatcgagaga cgccctccgg cacggtgcgt atcaatgcca gccagcacgc
     5401 gattgataaa tgcctgctgc caaagctggc ggtatttaaa cagcgatatc ctgatatccg
     5461 gcttgaactg ataaatgaga gccggttcgt cgatatcatc gaggaaagat tcgatgcagg
     5521 tgtccgcctg gggcctgaag tcggccaggg tatggtcgcg gtacgcatca cgcccgatat
     5581 ggagatggca attgtgggga ctccggaaca ttttcgtcgc tatggttttc cgcaaactcc
     5641 ggcggattta aaggcgcatc cctgtatcgc ctatcagttt gccgacggca gcgtatacca
     5701 gtgggaactc aatcaggatg ataaaaaaat cactcatcgc cctgaaggac aatgggccct
     5761 atccgacagc tatatggaag cagaagccgc ccggctgggc ctcggattgg cctatgttcc
     5821 tgttgaactg gtcgcggatg atttagaaca cggtaagctt atcagggtgt tgcagcgtta
     5881 cagcctgcga atggagggat tgtttctcta ttaccctcat cgcaacgtct cccccgccct
     5941 gcggatggtc attgatacat tgaaaatctg aacgttaata acgaccgact cttcactgtg
     6001 caaaattgtg tcaggtagtc cctctgcgtt caattccagg gtctgctttg agcgagaaag
     6061 atcagactta tctgtacaag gggactatcc agcaatggag atcatccaaa agaaaaaacc
     6121 acctggctcc cctcgacaac cttaatccgg gcatttagcc tcaatcaaca ttttcatatc
     6181 tgtcaggtta cttcattatg aaaaccgatc aatatcgttg tttttgttgc aacagtagaa
     6241 tacgcgtatt tatccctcca
//
LOCUS       DAZKFU020000257         6234 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZKFU020000257 DAZKFU020000000
VERSION     DAZKFU020000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.259"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            62..643
                     /locus_tag="ACHOQQ_RS20575"
                     /old_locus_tag="pgaptmp_004115"
     CDS             62..643
                     /locus_tag="ACHOQQ_RS20575"
                     /old_locus_tag="pgaptmp_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4472303.1"
                     /translation="MVSLVKNQTVSLSKESPALSQLHFGLGWDPVKKKGFLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLESTCKAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELACYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            643..1800
                     /locus_tag="ACHOQQ_RS20580"
                     /old_locus_tag="pgaptmp_004116"
     CDS             643..1800
                     /locus_tag="ACHOQQ_RS20580"
                     /old_locus_tag="pgaptmp_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4472304.1"
                     /translation="MNLMPGGNAPVPAQELRVRITSGVQVDASAFRLYAAGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGCVELSGRQEAALILGEFYRRNNGWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVPPLPAETKISLSKVSLTKEMPAISLTKRDNFGEIRINLNWH
                     RGSGKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIYVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            1823..2278
                     /locus_tag="ACHOQQ_RS20585"
                     /old_locus_tag="pgaptmp_004117"
     CDS             1823..2278
                     /locus_tag="ACHOQQ_RS20585"
                     /old_locus_tag="pgaptmp_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4472305.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFEPAEFGL"
     gene            2297..3337
                     /gene="terC"
                     /locus_tag="ACHOQQ_RS20590"
                     /old_locus_tag="pgaptmp_004118"
     CDS             2297..3337
                     /gene="terC"
                     /locus_tag="ACHOQQ_RS20590"
                     /old_locus_tag="pgaptmp_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HGU4472306.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPVWPKLRGHAFLLNQKEVDAELAKPENSDVTIDRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLIHLEKAVIVLLFFIAVKLGLNATDHIWHHGYSIAATTSLYVVLGVLVLGILASVI
                     FPGKPESEEKGN"
     gene            3386..3964
                     /gene="terD"
                     /locus_tag="ACHOQQ_RS20595"
                     /old_locus_tag="pgaptmp_004119"
     CDS             3386..3964
                     /gene="terD"
                     /locus_tag="ACHOQQ_RS20595"
                     /old_locus_tag="pgaptmp_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HGU4472307.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLTANGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDTVPSDI
                     DRIIFVVTIHDAQARRQNFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            4032..4607
                     /locus_tag="ACHOQQ_RS20600"
                     /old_locus_tag="pgaptmp_004120"
     CDS             4032..4607
                     /locus_tag="ACHOQQ_RS20600"
                     /old_locus_tag="pgaptmp_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4472308.1"
                     /translation="MAVSLVKGGNISLTKEAPTMNVAMVGLGWDARVTDGQAFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQIKIDLAKVSVDV
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGAEIARFDLSEDASTETAMVFGEL
                     YRHGAEWKFKAVGQGFSGGLSALASQHGVNV"
     gene            4721..6015
                     /gene="insG"
                     /locus_tag="ACHOQQ_RS20605"
                     /old_locus_tag="pgaptmp_004121"
                     /pseudo
     CDS             4721..6015
                     /gene="insG"
                     /locus_tag="ACHOQQ_RS20605"
                     /old_locus_tag="pgaptmp_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            6064..>6234
                     /locus_tag="ACHOQQ_RS20610"
                     /old_locus_tag="pgaptmp_004122"
     CDS             6064..>6234
                     /locus_tag="ACHOQQ_RS20610"
                     /old_locus_tag="pgaptmp_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HGU4472309.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMT
                     HHLGYEKNQSKPG"
BASE COUNT         1504 a         1483 c         1697 g         1550 t
ORIGIN      
        1 ttcgccagtt actaaatata cttttgattg tctcagttat cagcatttta aggaataaaa
       61 catggtttca ttagttaaga atcagacggt atcactcagc aaagaatcac ctgcattaag
      121 ccagcttcat ttcggtctcg gttgggatcc ggttaagaaa aaaggttttc ttggaggatt
      181 gtttggaggt aacgactcaa tcgatctcga tgctggctgt gtattaatgg acagcaccgg
      241 taaaacaatt gacaccatct ggttccgcaa actggaatcc acctgcaaag ctgttgtgca
      301 tagcggtgat aacctgactg gtgagggtga cggcgacgat gaagtgatta aggtcaacct
      361 ctcccgtctg cctgcaaacg ttgaatacct ggcgtttacg gttaacagct tccgtgggca
      421 gtcgttcaat gacgtcgaaa atgcgttctg tcgtgtggtc gatcaaaccg gcaaagagct
      481 ggcttgctac aagctgactg agcagggctc acataccggg atcgttattt cttcgctgcg
      541 ccgtaataac ggcaactggg actttaccgc ccttggtcat gcctgtcgcg gtcgcacaat
      601 tgacgatatg cactccgata tcgtttctgc ggttattcgc taatgaacct gatgccgggg
      661 ggcaatgccc ccgttccagc ccaggagttg cgggtccgga taacctcggg cgtccaggtg
      721 gatgcttcag cgtttcgcct gtatgccgcc gggaaggtgc agggggatgc ggacatggtg
      781 ttttatggtc agccacgtaa cgatgatggt actgtcagcc tggtcagtga aggtcagtac
      841 tcaaccttta ctgtcgcgct caaccggctg aaacctgatg tccagaaaat tgcgttcacc
      901 gtgacctgtg acggtgggca gacggtgtcc ggtcttcgta atctttccat cgacgtggag
      961 caaggggcta ccggcctggt gagcggctgt gtcgaactga gcggccgtca ggaagcagcc
     1021 ttaattctgg gtgaatttta ccgtcgtaat aatggctgga aattccgctt cgttgcgcag
     1081 gggttcaacg gtggacttaa accgctggca gagcatttcg gcgtaaatat tgccgacgaa
     1141 ccggctcccg cagccccgac tcctccggtt gtaccccctc tgccagctga gacaaaaatc
     1201 agcctgagta aagtatctct gacaaaagag atgcctgcaa tcagtctgac caaacgggat
     1261 aacttcggtg aaatcaggat caacctgaac tggcaccggg gcagcggcaa atccggcttt
     1321 gcaggcatgt tcggctccaa aggcatcgac ctggatctgg gagcctttgt tgagcttcag
     1381 gacgggtata agtcggtcat tcaggcgctc ggtaatgcat tcggtgatta tcgcgatgaa
     1441 ccttatgttc agctcaaggg cgatgaccgg acgggtgatg tatcagacgg cgagtggctg
     1501 catatcaatg gacgtgaatg gaagcatatc cgcgaagtgc tgatttacgc ctttatttat
     1561 gaaggtgttc ccagttggga caagactgat ggagtggtca ccatttatgt gccggatcaa
     1621 ccgcccattg agacccgcct gactgaaggt gaaaaccgtc gcacattgtg cgccattgcc
     1681 agactcgtaa acgaaaacgg tgcgattaaa gttgagcgta tcaaccagta cttcaaaggc
     1741 caggacgaaa tggaccgggc atttggctgg ggatttcgct ggagcgccgg ttctaaataa
     1801 cacagcaata aaggaaactg gtatgagctt tttcgacaaa gttaaaggtg ccattaactc
     1861 tggtcgtgac gaactgaccc gccaggttgg tcgtttcaaa aacaaaaaat tcatgcaggg
     1921 caccgttgct gtatgtgccc gtattgccgt atcgagtgac ggcgtgagtt cggaagaaaa
     1981 gcagaaaatg atgggctttc tgcgctcttc agaagagctg aaggtcttcg ataccaatga
     2041 ggtgatcgag ttcttcaata aactggtttc aagcttcgat ttcgatgttg aaatcggcaa
     2101 gggcgagacc atgaaataca tcctggcgct gaaagatcag cctgaggccg ctcaactggc
     2161 cttacgtgtt ggtattgctg ttgcgaaaag cgacggtaac ttcgatcagg acgagaaact
     2221 ggcctcccgc gagatcgcta tcgcgttggg cttcgagccg gctgaatttg gcctctgaca
     2281 tccctaaggg taaaatatgg tttccacaca catcggcttc ccgactgaaa cggtcattgt
     2341 ttttattgct ctttcagtcg gagccatctt tattgacctg tttatgcacc gtgatgacaa
     2401 gccaatctcc ctgaagagtg cggcgctctg gtccgtattc tgggttgttg ttgcgatggc
     2461 atttgccggt ttcctctata tccaccacgg tgctgaagtt gccagtttgt ttgtcacggg
     2521 gtatgcgctg gagaaagtac tgtcggtcga caacctgttt gtcatgatgg ccatcttttc
     2581 ctggttcgct gttccggatc gttatcggca ccgtgtgctt tactggggga tcattggagc
     2641 gatcgtcttc aggggaatct ttgtcgccat cggtacgagc ctgctgagtc tggggccgta
     2701 cgttgaagtc gtcttcgcta tcatcgttgc ctggacagcg gtcatgatgc ttaaaagcgg
     2761 tgatgatgat gatgaaattg aagattactc ccagcatctg gcttaccgca tggttaaacg
     2821 tttcttccct gtctggccga agctacgtgg gcatgccttc ctgcttaacc agaaggaagt
     2881 ggatgctgaa ctggcgaaac cagaaaacag tgatgtaacc attgaccgtg gtaaaaaggc
     2941 ggcgctgtat gcgacccctc tgttcctgtg tgtggccgtg gttgaactct cggatgttat
     3001 gtttgcgttt gactcggtac cggcaatcat tgctgtcagt cgtgaaccgc ttatcgtcta
     3061 tagtgccatg atgtttgcta tcctggggct gcgtactctg tactttgtcc ttgaagcact
     3121 gaaacagtac ctgattcatc tggaaaaggc tgttatcgtg ttgttgttct tcattgcggt
     3181 aaaactgggc ctgaacgcga ccgatcacat ctggcatcat ggttacagca tcgctgcgac
     3241 aaccagcctg tatgttgtac tgggggttct ggtgctgggc attctcgcaa gcgttatttt
     3301 ccccggcaaa cctgaatctg aggaaaaggg gaactgatcc ccttaagtgt taaccggagc
     3361 aattactaat cgaagaggtt attttatgag tgtttctctt tccaaaggcg ggaacgtctc
     3421 cctgagtaaa gcagctccgt caatgaaaaa cgtcctggtg ggtcttggct gggatgcacg
     3481 ttcaactgac ggtcaggact ttgacttgga tgcttcagca ttcctgctga ccgcaaacgg
     3541 caaagtgcgt ggcgattcag atttcatctt ctataacaac ctgacgtcat ccgacggttc
     3601 tgtaacgcac accggcgata accgtactgg tgagggggat ggtgatgatg aatcgctgaa
     3661 aattaaactt gatactgtcc cgtctgacat cgacaggatc atcttcgttg tgacgatcca
     3721 tgatgctcag gctcgccgtc agaactttgg tcaggtttcc ggggcgttta ttcgcctggt
     3781 taatgacgat aaccagactg aagttgctcg ctacgatctg actgaagatg cgtccactga
     3841 aaccgccatg ctgttcggtg aactgtatcg tcacaatggt gagtggaaat tccgtgctgt
     3901 aggtcagggt tatgcggggg ggctggcatc tgtatgtgct cagtacggca ttaacgcgtc
     3961 ttgatcgaaa actaactttt cattccgggc tgttgattca gccctcattc aactgtttgc
     4021 aggagttaaa aatggcagtt tctctcgtaa aaggcggcaa catatctctg accaaagaag
     4081 caccgaccat gaatgttgct atggttggtc ttggctggga tgcccgtgta actgacggtc
     4141 aggcttttga tctggacgct tccgtattcg cagtaggtga agacggtaaa gtgctgtcag
     4201 atgcccattt cattttcttc aataataaaa ccagccctga tggcgcagta gagcaccagg
     4261 gtgacaaccg taccggtgaa ggcgatggtg acgatgagca aattaaaatc gatctggcaa
     4321 aagtctcagt tgacgtcaaa aaactggtat ttgccgttac catctatgat gcagaagcgc
     4381 gtaaacaaaa tttcggcatg gtgagcaaca gcttcatgcg cgtttacaac aacgataacg
     4441 gcgcggaaat tgcccgtttc gatctgtctg aagacgcctc aactgaaacc gcaatggtct
     4501 tcggtgaact gtatcgccat ggcgctgagt ggaagtttaa agctgtcggt cagggttttt
     4561 ccggtggcct gtcagcgctt gcttcccagc acggcgtcaa cgtctaaaag atgcttagtc
     4621 tgaccccggc aatgccgtaa tgccgatcgg ttaaggatca gttgaccgat ccagtggttg
     4681 tgtaagaatc cggaaacgct cactcgtttc cggatttttt atgcacactg gacaggccct
     4741 tgatctggta tcccgttacg attctctgcg taacccactg gcttctctgg gggattacct
     4801 cgaccccgaa ctcatctctc gttgccttgc cgaatcaggt acggtaacgc tacgcaagcg
     4861 ccgtcttccc ctcgaaatga tggtctggtg tattgttggc atggcgcttg agcgtaaaga
     4921 gccccttcac cagattgtga atcgcctgga catcatgctg ccggcaatcg ccccttcgtt
     4981 gcccccagtg ccgttattca ggcccgccag cacctgggaa gtgaggctgt ccgccgcgtg
     5041 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg atgcggcctg
     5101 accctgctgg ccatcgatgg tatgttctgg cgcacaccgg atacaccaga gaacgatgca
     5161 gccttccccc gccagataca tgccggcaac ccggcgctct acccgcaggt caaaatagtc
     5221 tgccagatgg aactgaccag ccatctgctt acggctgagc cttcggcacg atgaagaaca
     5281 gcgaaaatga gcttgctgag caacttatag aacaaaccgg cgataacacc ctgacgttaa
     5341 tggataaagg ttattactca ctgggactgt taaatgcctg gagcctggtg ggagaacacc
     5401 gccactggat gatccctctc agaaagggag cgcaatatga agagatcaga aaactgggta
     5461 aaggcgatca tctggtgaag ctgaaaacca gcccgcaggc acgaaaaaag tggccgggac
     5521 tgggaaatga ggtgacagcc cgcctgctga ccgtgacgcg caaaggagaa gtctgccatc
     5581 tgctgacgtc gatgacggac gccatgcgct tccccggggg agaaatggcg gatctgtaca
     5641 gtcatcgccg ggaaattgaa ctgggataca gggagataaa acagacgatg caactgagca
     5701 ggctgacgct gtaaaaagcc ggagcttgtg gagcaagagc tgtggggtgt cttactggct
     5761 tataatctgg taagatgtca gatgattaaa atggcggaac atctgaaagg ttactggccg
     5821 aatcaactga gtttctcaga atcatgcgga atggtgatga cattgcaggg cgcttcaccg
     5881 ggacgtatac cggggctgat gcgcgatctt gcaagtatgg gacaacttgt gaaattaccg
     5941 acaagaaggg aaaggggctt cccgagagtg gtaaaggaga ggccttggaa agagcctgta
     6001 catagatttg tgtaattgcc tgattttgat atgttcaatc aagcatcaaa tgaaggttaa
     6061 tttatggacg aaaaacagtt acaggctctg gctaacgaac tggccaaaaa cctcaaaacc
     6121 cctgaagacc tcagccagtt tgaccgtcta ctgaaaaaga tcagcgttga agccgctctc
     6181 aatgcagaga tgacacacca tcttgggtat gagaaaaatc agtccaaacc ggga
//
LOCUS       DAZKFU020000258         6227 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZKFU020000258 DAZKFU020000000
VERSION     DAZKFU020000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.260"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..1340
                     /gene="wcaK"
                     /locus_tag="ACHOQQ_RS20615"
                     /old_locus_tag="pgaptmp_004123"
     CDS             60..1340
                     /gene="wcaK"
                     /locus_tag="ACHOQQ_RS20615"
                     /old_locus_tag="pgaptmp_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGU4472310.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FHDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAHQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSCERQTGMQMVQSVLERIGEVK"
     gene            1337..2557
                     /gene="wcaL"
                     /locus_tag="ACHOQQ_RS20620"
                     /old_locus_tag="pgaptmp_004124"
     CDS             1337..2557
                     /gene="wcaL"
                     /locus_tag="ACHOQQ_RS20620"
                     /old_locus_tag="pgaptmp_004124"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGU4472311.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVDIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVVTPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISNLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            2568..3962
                     /gene="wcaM"
                     /locus_tag="ACHOQQ_RS20625"
                     /old_locus_tag="pgaptmp_004125"
     CDS             2568..3962
                     /gene="wcaM"
                     /locus_tag="ACHOQQ_RS20625"
                     /old_locus_tag="pgaptmp_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGU4472312.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAASFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            4120..5115
                     /locus_tag="ACHOQQ_RS20630"
                     /old_locus_tag="pgaptmp_004126"
     CDS             4120..5115
                     /locus_tag="ACHOQQ_RS20630"
                     /old_locus_tag="pgaptmp_004126"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HGU4472313.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKSPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            5358..>6227
                     /gene="galF"
                     /locus_tag="ACHOQQ_RS20635"
                     /old_locus_tag="pgaptmp_004127"
     CDS             5358..>6227
                     /gene="galF"
                     /locus_tag="ACHOQQ_RS20635"
                     /old_locus_tag="pgaptmp_004127"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGU4472314.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGIKFREK"
BASE COUNT         1660 a         1551 c         1566 g         1450 t
ORIGIN      
        1 aaaacggcaa cgaacacaac aaacacaacc tcacctggca tcaaatttat gaggaaaaaa
       61 tgaaattact tattctgggc aaccacactt gcggcaatcg tggcgacagc gccatcctgc
      121 gcggcttact tgatgccatc aacatcctca atccacacgc cgaagtggac gtcatgagcc
      181 gctatccggt cagttcttcc tggctgctca accgcccggt aatgggcgac ccgctgttcc
      241 tgcaaatgaa acaacacaac agcgcggcgg gcgttgtcgg gcgcgttaaa aaagtcctcc
      301 gtcgccgcta ccagcatcag gtattgctct cacgcgtcac cgacactggc aagctgcgta
      361 atatcgccat cgcccaggga ttcaccgact tcgtgcgcct gctgtcaggt tacgacgcca
      421 ttatccaggt cggcggatcg ttttttgtcg atctctacgg cgtgccgcag tttgaacatg
      481 cgctttgcac atttatggcg aaaaagccgc tgtttatgat tggtcacagc gtcggcccgt
      541 tccatgatga gcaatttaac caactggcga actacgtttt cggtcactgt gacgcgctga
      601 tcctgcgcga atcggtcagc ctcgatctaa tgaaacgcag caatatcact accgcaaaag
      661 tggaacatgg cgtcgatact gcgtggctgg tcgatcacca caccgaagat ttcaccgcca
      721 gctatgccgt ccaacactgg ctggacgttg ccgcacacca gaaaacggtg gcaattaccc
      781 tgcgcgaact ggcaccgttc gacaaacgcc tcggcaccac tcagcaagcg tatgaaaaag
      841 cctttgccgg ggtggtcaat cgcattctcg acgaaggcta tcaggtcatt gcgctctcca
      901 cctgtacggg tatcgacagc tataacaaag acgaccgcat ggtggcgctc aacctgcgcc
      961 agcacatcag cgatcctgcc cgttaccacg tagtgatgga tgaactcaac gatctggaaa
     1021 tgggcaaaat tctcggtgcc tgtgaactca ccgtcggtac gcgtctgcac tccgctatta
     1081 tctcaatgaa ctttgctact ccggcgattg ccatcaacta tgaacataaa tccgccggga
     1141 tcatgcaaca actggggctg ccagagatgg cgattgatat ccgtcattta ctggacggca
     1201 gcttgcaagc gatggttgcg gataccttag gccaacttcc ggcgctgaac gcgcgactta
     1261 acgaagccgt cagttgcgaa cgtcagacgg gaatgcagat ggtgcagtct gtactggaac
     1321 gcatcgggga ggtgaaatga aggtcggctt cttcttattg aaatttccgc tgtcgtcgga
     1381 aaccttcgtc ctcaatcaaa ttaccgcgtt tattgatatg ggatttgagg tggatattgt
     1441 tgcgctgcaa aaaggcgaca cacaaaacac ccacgcggca tggacgaaat ataaccttgc
     1501 cgccagaacc cgctggttac aggacgaacc tacgggcaaa gtggcgaaac tgcgccaccg
     1561 cgccagccag acgttacgcg gcattcatcg taaaaatacc tggcaggcgc tcaacctcaa
     1621 acgctatggt gccgagtcgc ggaacctgat tttgtctgcc atttgcggcc aggtcgtaac
     1681 accgcttcat gccgatgtct ttatcgctca ctttggtcca gcaggggtaa ccgcagcaaa
     1741 actccgcgaa ctgggtgtca ttcgcggcaa aattgctact atcttccacg gcattgatat
     1801 ctcaagtcgg gaagtgctca accactacac ccccgaatat caacaactgt ttcgccgtgg
     1861 cgacctgatg ttaccgataa gtaatctgtg ggccggaagg ctgcaaaaaa tgggctgccc
     1921 gagggaaaaa atcgccgtat cgcgtatggg tgtggacatg acgcgcttta gcccgcgtcc
     1981 ggtgaaagcg cccgcaacac cgctggaaat catttccgtc gcacgcttaa ccgagaaaaa
     2041 aggcctgcat gtggcgatcg aagcctgccg tcagttgaaa gagcagggcg tggcatttcg
     2101 ctatcgcatc cttggcatcg gcccgtggga acgacgcctg cgcaccctca tcgaacaata
     2161 tcaactggaa gatgtggtag agatgccggg ctttaaaccg agccacgaag tgaaagcgat
     2221 gctcgacgac gcggatgtct tcctgttgcc atcggtaacg ggcgcggatg gcgatatgga
     2281 aggcattccg gtagcgctga tggaggcgat ggcggtcggc attccggtgg tttctactct
     2341 gcatagcgga ataccggaac tggtggaggc tgacaaatcc ggctggctgg tgcctgagaa
     2401 cgatgctcgc gcactggcgc aacgactggc ggcgtttagc caactggaca ccgacgaact
     2461 ggctccggtc gtcaaacgtg cgcgcgaaaa agtcgaacat gattttaacc agcaggtgat
     2521 caatcgagaa ctcgcaagct tgctgcaagc tttatagagg agaacgcatg ccatttaaaa
     2581 aactctcccg acgcaccttc ctgacggcaa gctcggcgct tgccttcctc catacccctt
     2641 tcgctcgcgc acttcccgcc cgacaaagcg ttaacattaa cgactacaac ccacacgact
     2701 ggatcgcctc atttaaacaa gccttcagcg aagggcaaac ggtcgtcgtg cctgctggat
     2761 tcgtttgtga caatatcaac accggcatct tcattcctcc tggcaaaacg ttacacatcc
     2821 ttggaagcct gcgcggcaac ggcagagggc gatttgtctt acaggacggc agccaggtga
     2881 caggggagga gggcggcagt atgcataaca tcaccctgga tgtgcgtggt tctgactgca
     2941 ccatcaaagg gctggcgatg agcggctttg gtccggtaac gcagatttat atcggcggca
     3001 aaaacaaacg ggtcatgcgc aacctgacca tcgataacct gaccgttagc cacgctaatt
     3061 acgccatctt acgccaggga tttcataacc agattatcgg tgccaacatc accaattgta
     3121 agttcagcga cttacagggc gacgccattg aatggaacgt ggcaattaac gacagtgata
     3181 ttttgatctc cgaccatgtc atcgagcgca tcaactgtac taacggcaaa atcaactggg
     3241 gcattggcat aggccttgcg ggaagcactt acgataataa ttacccggaa gatcaggcag
     3301 tgaaaaactt tgtcgtggcg aatatcacgg gatcggattg tcggcagttg atccatgttg
     3361 aaaatggcaa acattttgtt atccgtaata tcaaagcccg caatatcacg ccggatttca
     3421 gtaagaaagc gggcattgat aacgcgacag tcgctattta cggttgtgac aatttcgtga
     3481 ttgataatat tgaaatgatt aatagcgccg ggatgttaat cggctatggg gtaattaaag
     3541 gcaaatatct ctcgataccg caaaatttcc gagtgaataa tattcaactg gataacaccc
     3601 atcttgctta taaattgcgc ggcatccaaa tctccgccgg gaatgctgcc tcctttgtgg
     3661 cactgactaa cattgagatg aagcgtgcct cgctggagct tcacaataaa ccgcaacatc
     3721 tttttatgcg taatatcaaa gtgatgcagg aatcctcagt tggtcccgca ttgagcatga
     3781 acttcgacat gcgcaaagac gttcgcggcg tgtttatggc gaaaaaagaa acactgctgt
     3841 ctcttgcaaa tgttcatgcg gtgaatgaaa gagggcaaag ctccgtcgat atcgacagga
     3901 ttaatcacca tattgttaat gtggaaaaga ttaactttag attgccggaa cggagagagt
     3961 agatttgcga ccattcataa acttactgga tataatgttc taaaagatta taatcctcac
     4021 actaactgtt aaagctgggg ctgggcagca ttgactactc agggaaggta ctcatcttta
     4081 tcttgtgttt cttacgtaac aaccagacta aagtgattta tgaacgataa cgttttgctc
     4141 ataggagctt ccggattcgt aggaacccga ctacttgaaa cggcaattgc tgactttaat
     4201 atcaagaacc tggacaaaca gcagagccac ttttatccag aaatcacaca gattggcgat
     4261 gttcgtgatc aacaggcact cgaccaggcg ttagccggtt ttgacactgt tgtactactg
     4321 gcagcggaac accgcgatga cgtcagccct acttctctct attatgatgt caacgttcag
     4381 ggtacccgca atgtgttggc tgccatggaa aaaaatggcg ttaaaaatat catctttacc
     4441 agttccgttg ctgtttatgg tttgaacaaa cacaaccctg acgaaaacca tccacacgac
     4501 cctttcaacc actacggcaa aagtaagtgg caggcagagg aagtgctgcg tgaatggtat
     4561 aacaaatcac caacagaacg ttcattaacc atcatccgtc ctaccgttat cttcggtgaa
     4621 cgcaaccgcg gtaacgtcta taacttgctg aaacagatcg ctggcggcaa gtttatgatg
     4681 gtgggtgcag ggactaacta taagtccatg gcttatgttg gaaacattgt tgagtttatc
     4741 aagtacaaac tgaagaatgt tgccgcaggt tatgaggttt ataactacgt tgataagcca
     4801 gacctgaaca tgaaccagtt ggttgctgaa gttgaacaaa gcctgaacaa aaagatccct
     4861 tctatgcact tgccttaccc actaggaatg ctgggtggat attgttttga tatccttagc
     4921 aaaattacgg gcaaaaaata cgctgtcagc tcagtgcgcg tgaaaaaatt ctgcgcaaca
     4981 acacagtttg acgcaacgaa agtgcattct tcaggttttg tggcaccgta tacgctgtcg
     5041 caaggtctgg atcgaactct gcagtatgaa ttcgttcatg ccaaaaaaga cgacataacg
     5101 tttgtttctg agtaatgcag acgagtcaga gaaaaaagga ccaggttgga ctacgattat
     5161 tcggaaagag gtactgatta aattatcagt atgttgattt ccgagtaatc cgagtaaccc
     5221 agatgctgat ggtgtgtagt gttatcgtaa ggtttaatca aagttatgcg gaactaagct
     5281 ttggtatata gccataacgt ttggctaacc tttggcaata attcacgcac tatcagccac
     5341 ttagaggtag gaaaagaatg acgaatttaa aagcagttat accggtagcg gggcttggga
     5401 tgcatatgtt gcctgccacc aaggcgattc ctaaagagat gctaccgatc gtcgacaagc
     5461 ctatgattca gtacattgtt gacgagattg tggctgcagg gatcaaagaa atcctcctgg
     5521 taacgcatgc gtccaagaac gcggtcgaaa accacttcga cacttcttat gaattagaat
     5581 cactccttga acagcgtgtg aagcgccagc tgctggcgga agtacagtcc atctgcccgc
     5641 cgggcgtgac aattatgaac gtgcgtcagg gtgaaccttt gggattaggt cactccattt
     5701 tgtgtgcacg acccgccatt ggtgacaatc cgtttgtcgt ggtgctgcca gacgttgtga
     5761 tcgacgacgc cagcgctgat ccgctgcgct acaaccttgc tgccatgatt gcgcgcttca
     5821 atgaaacggg ccgtagccag gtgctggcaa aacgtatgcc gggtgacctc tctgaatact
     5881 ctgtcatcca gaccaaagag ccgctggacc gcgaaggtaa agtcagccgc attgttgaat
     5941 tcatcgaaaa accggatcag ccgcagacgc tggactcaga catcatggcc gttggtcgct
     6001 atgtgctttc tgccgatatt tggccggaac tagaacgtac tcagcctggt gcatggggac
     6061 gtattcagct gactgatgcc attgccgagc tggcgaaaaa acagtccgtt gatgcaatgc
     6121 tgatgaccgg cgacagctac gactgcggta aaaaaatggg ctatatgcag gcgtttgtga
     6181 agtatgggct gcgcaacctg aaagaaggta ttaagtttag agaaaaa
//
LOCUS       DAZKFU020000259         6151 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZKFU020000259 DAZKFU020000000
VERSION     DAZKFU020000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.261"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(43..558)
                     /gene="ogt"
                     /locus_tag="ACHOQQ_RS20640"
                     /old_locus_tag="pgaptmp_004128"
     CDS             complement(43..558)
                     /gene="ogt"
                     /locus_tag="ACHOQQ_RS20640"
                     /old_locus_tag="pgaptmp_004128"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGU4472315.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(569..2095)
                     /gene="abgT"
                     /locus_tag="ACHOQQ_RS20645"
                     /old_locus_tag="pgaptmp_004129"
     CDS             complement(569..2095)
                     /gene="abgT"
                     /locus_tag="ACHOQQ_RS20645"
                     /old_locus_tag="pgaptmp_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HGU4472316.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(2132..3577)
                     /gene="abgB"
                     /locus_tag="ACHOQQ_RS20650"
                     /old_locus_tag="pgaptmp_004130"
     CDS             complement(2132..3577)
                     /gene="abgB"
                     /locus_tag="ACHOQQ_RS20650"
                     /old_locus_tag="pgaptmp_004130"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HGU4472317.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPISKNVTPSPLK"
     gene            complement(3577..4887)
                     /gene="abgA"
                     /locus_tag="ACHOQQ_RS20655"
                     /old_locus_tag="pgaptmp_004131"
     CDS             complement(3577..4887)
                     /gene="abgA"
                     /locus_tag="ACHOQQ_RS20655"
                     /old_locus_tag="pgaptmp_004131"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HGU4472318.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            5063..5971
                     /locus_tag="ACHOQQ_RS20660"
                     /old_locus_tag="pgaptmp_004132"
     CDS             5063..5971
                     /locus_tag="ACHOQQ_RS20660"
                     /old_locus_tag="pgaptmp_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4472319.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVREILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
BASE COUNT         1575 a         1598 c         1542 g         1436 t
ORIGIN      
        1 ttacataaaa gtgtgaacaa gctggcacaa attgtttaat gtttacagca aaagataacc
       61 ttcgtggcgc aataaccact cttttcgctg aactccgcct gcatatccgg tcatggtgcc
      121 gtttcggcca ataacccgat ggcaaggtac gacgatgctg atgggattcg atccgtttgc
      181 cgcaccaacg gcacgcgccg cgccaggacg gcccaattgc tcagccagtt cgccgtaatg
      241 cattacctgc ccgcagggga tagtgcgtag tgttttccag acttcgcgct gaaatggcgt
      301 cccccccgta gcagtgggaa gcgtatcaat aatgctaaga ttaccggcaa aatattcacg
      361 aagcttgtcg cttaaaccgc ctggattggt ggcagaaatg cgctcatagc cttctttgcg
      421 ataatggatg tccagcagct gcaccatgcg ttcgctgtac tcttcccatt caaccgcccg
      481 caggcgaaat tgctcatcgc aaatcaccca cagtggaccc agtggcgtgg caattttttc
      541 ttcaagtaat ctcagcatcc gttctctctt aagacaaacg tgggtaaata cccggaccta
      601 tcggcaggcc cacaagatac cacgccagca acattagcag ccataccacc aaaaagataa
      661 gcggataggg caagactaac gaatagtaag tacccagttt cgcgtctggt ttgtagcgtt
      721 gcaggaatcc aagaaacagt ggaacaaaag gagatactgg cgctaaaggc aatacggatg
      781 agtcggcaat acgaaagagg atttgcgcaa atgccgggtg aaagccaagt agcataaaca
      841 ttggtacgaa aatgggggcc agaatcgacc agattgcgga accgctggcg ataaacatgc
      901 ataagaaaga ggaaagcaac gccagaccga caaacgccgg gatgccgcta agccctgaac
      961 tttccagtat atcggtcagc cccacggcga tgaatttccc catgttgctc cagttaaaca
     1021 tggcgacaaa ctgggcgagg ggaaaaacca tcacgataaa tcccgccatc tctttcatcg
     1081 gttcaatcat taaatgcggt aaatccgcct gacgtcgaat tgtgcgggta gcgatgccat
     1141 aagccagcga gacaacaaag aaaaaaagaa tgatcagtgg cacgatacct ttaataaagg
     1201 gtgatggcat cacggtgtga ttaatcggat cgcgcaatat cccgttttcc gggatcacca
     1261 tcagcgcaat cgcagcaata aaaagtagcg atacgacacc tgctatgcgt aaaccaaaac
     1321 gctgactttc ggtcaatgtc tgcagtttct catcgctgtt tccctgccat tgacctaacc
     1381 gtggctcgat gattttgtcg gttatcaggc cgccaacaat cgtcagtacg actacggagc
     1441 tggccataaa ataccagtta tcaattacac tgacgtgcat ttgcggattg aacgcagccg
     1501 ccgcttccgt gctgatcccc gacagcaaca cgtcggttgt gacaatcagt aaattagccg
     1561 taaagccgca acctacgcct gcaatggcag ccagtaaacc tgcaactgga tgcctgccca
     1621 ccgccagaaa aatcagcgca cccatcggtg gcatgatcac taacgccgca tcggaagaaa
     1681 tgtggctgaa aaaagcaata aacagcacca tataactggc gtagcgggca ttaacatgcg
     1741 atgccatttt aaccattagc gctggcagta agccgacgcg ctccgccaga ccggcaccta
     1801 aaaccagcgc caggatcgca ccaagtggag caaaaccgct aaagttttta ataacattgg
     1861 gtaaaaacca gtgtaatcct tccacactga gcaggttttt caccaccacc ggcgtaccat
     1921 cggtcgggtt tttcgcactg acgccaaagg ccgacaaaat tgccgtcgtc accatgagta
     1981 cgataatcaa atagataaag agcaaaaaag gatggggaac cttgttacca attctttcga
     2041 cccagccata gagcttcccg gattgggagg acgacggtat ggatgacata ctcatgggca
     2101 ttcctcggtt gttgtgtttg cgttgttgtt gttattttaa aggtgacggt gtcacgtttt
     2161 tcgagatagg gcagtgatac ggttgcgtgt ccgtaacttg ctgatgctct tgttggcatt
     2221 cttgcaatag ccctgaatca atgaagagat tgagtgtggt tgctgccata gttttcgccg
     2281 ccagcagcat tcctttatga gcaatagatg ttcgcccctg gctaaccagt tgccacgtat
     2341 gtagcggtgt accgacggca aaacaggggc tgaaacactg ggcaacaggc agtttccaac
     2401 tgacgtcgcc gacatcagtc gatgccgcaa gcacgttatc ggtggcggca tatggagcga
     2461 cttcattcgc cagtaccgtt tcacgatgac gtagtgcaaa agccttgccg ttttcgccac
     2521 cggttgcggc gatattattc agactgtttt gccgatcgtt gggggtgagc gtagcctgaa
     2581 tttgtttcgc aaaagccagt tcttcggagt tccattccgg ggtaccaaaa tgggataggg
     2641 cctggtacat ggcattttct aaggtgcgat tcgggagata actggaacag gctttgtcga
     2701 agcggcattc aaccgtggtt tcggtcatca atgccgcacc ttcggcgatt ttggcgactc
     2761 gatcataaat atgctgcacg tcggtcattt cgggggcgcg gataagataa agcacttctg
     2821 cctgcgcctg gaccacgttg ggcgagatcc cgccactatt tgtgatggca tagtgtacgc
     2881 gcgctttttc aataatatgt tcgttgagga agttggtgcc agtggtcatc aacgttacgg
     2941 catcaagggc gctgcgtccc aaatgagggg aattcgcggc atgtgctgcg atccctttaa
     3001 agcgccatga tgcctgaatg tttgccagcg tacgggtatt gaacataccg gcaaaggctt
     3061 ccgggtgcca ggtgagtgcc gcatccacat catcaaatac cccctcgcga accatgaacg
     3121 ttttacccga gccgccttct tcgccaggac aaccataaaa gcgcaccgtg ccgccttgcc
     3181 catattgttc cagccatttc ttgacggcta ttgcagcggc aaaggcggcg gttcccagca
     3241 aattgtgtcc gcaaccgtga ccattttcac cgggcgtcac ggatgtaggt tgcgcgcaac
     3301 ctgcttgctg acttaaacct gccagggcgt catattctcc cagcagggcg ataaccggtt
     3361 tgccttgacc aaacgaagca ataaaggcat ttgggatatt gcctacgttg cgggtaacgg
     3421 tgaagcctgc agattccagc gccgaagcca gatgctccgc tgaccagaac tcttcaaaac
     3481 gtgtttctgg atgatcccag atttgatcgg caatatcggt ataacgttgg cgatcggctt
     3541 caatcgcatc gtcgataaaa cgatagattt cctgcatcag atacctcgcg tccagggaaa
     3601 attgagcgcg gtgcgcgcca gcgtttcgac ggcaatagcg agaacctgct cgtcaaaatc
     3661 gaatttttcg ttgtgatgac ctgccgccag ctgtgtgcca aacaccatgt aggaggcttg
     3721 cccttgatgt tgctgcacgc gggccatcat taatgtggca tcttcggaac ccgcaggcgc
     3781 ttcaacacgt tcaatggcct gattgacccc cgcgacctga gccgcctgac tttgcaacca
     3841 tgcgacccat tgcggcgaag gagaactggc ggtagctgca cccatcagac gagtttcaac
     3901 gccgacacca tacatggttg ctgcgccctg aatcgcttgc tgagcacggt caaaaacata
     3961 ttgattaatg acgtcgctgg ccccgcgtgt ttccactttc agcaacgccg aggcaggaac
     4021 aacattacga ccgcttcctg cctgcataac gcccacgttt actctggaag cgccttcgct
     4081 gtgcggggcg attgcatgta gtgcaagagt tgcttgtgct gccgccaaca aggcattgtg
     4141 accgtcttct ggttttgcgc ctgcgtgagc ggcggtcccg gtaaagtgcg cgtcaaattt
     4201 ggtggttgcc ataaaattat cactgccgca caccacggtg cccgcaggta cgccagtgcc
     4261 aatgtgcacg gcagtaaaat aatcaacatc atccactaca cctgcatcga ccatcgcccg
     4321 cgcgccacgc gtaccttcct ctgcaggctg aaaaatcagt ttgatgacgc catgtagtcc
     4381 ggactcgaac tgtttaaggg tatgcgccag cccaagccca atggcggtat gcccatcatg
     4441 accacaggca tgcatcattc cggcgttaca tgacgcaaaa ccgtcgcggt aggggcgatg
     4501 gctgacatcc tgctcttcac tgagatccag cgcgtccata tcgacacgga aagccatcac
     4561 cggaccgggg cgaccggtat ccagggtggc gacgatacca gtgaaaccac cttcaaaagc
     4621 cgcaatccat tgtgctagcg caccctgttg acgagcgcgc tcgaattcgc gttgtagagt
     4681 gaattcatca ggtaatccca tccggctact ttcattaact acttcgcgac ccagcgccag
     4741 tgaatagccg agctggtgca attcttccgc aacaagggtg gcagtgcgga attccaccca
     4801 gccagactct gcatagtgat gaaaatcacg tcgccagtgc gataattttg gggcaagcga
     4861 attaacaaat tgattcaaag actccataac ctttcccgtc atcagtaaaa agtgtgaccc
     4921 ggttcacgta gcgatagttt ttacttatca ctaactgatt tttcacagtt ttaaccgttc
     4981 ataaattacc ctgacacaat catctgcatt aaagtagatg ccagtttctt tggtctgata
     5041 aataacggtt atcggtggcg tcatggcttt tcaggtaaaa attcatcaaa ttcgggcttt
     5101 tgttgaagtg gctcgtcagg gcagcattcg cggagcgagc cgaatgttga atatgtcgca
     5161 accggcactg agtaaatcta ttcaggagct agaagaaggg ttagcggcgc aactcttttt
     5221 tcgccgtagt aaaggcgtga cgttaactga tgccggtgaa agtttttatc agcacgccag
     5281 tctaattctt gaagagctgc gcgcagccca agaggatatt cgccaacgac aagggcaact
     5341 ggcagggcag attaatatcg gcatgggggc cagtatttcc cgcagtctga tgccagctgt
     5401 catatctcgt tttcatcagc agcatccgca ggtaaaagta cgcattatgg aagggcaact
     5461 ggtgtcgatg attaatgaat tgcgtcaggg agaattggat ttcaccatca atacctatta
     5521 tcagggaccg tacgaccacg aatttacttt tgagaaatta ctggaaaagc aattcgcgat
     5581 cttttgccgc ccgggacacc ccgccattgg tgcccgttcg atcaaacagt tactggatta
     5641 cagctggaca atgccgacgc cacacggcag ctactacaaa cagttgagtg aattgcttga
     5701 cgatcaggcg caaacgccac aggtcggtgt agtctgcgag acgttctcag cctgtatcag
     5761 tctggtggca aaaagcgatt ttctcagcat actgcctgaa gaaatgggct gcgatccctt
     5821 gcacggacag gggctggtga tgttgccggt tagagaaatt ttaccgaaag cggcctatta
     5881 tttgattcag cggcgtgata gtcgccagac accactgacc gcgtcattaa tcacgcaatt
     5941 ccggcgagaa tgcggctatc tgcaaagtta aaactgcata aaaaaataga gtctgtcgac
     6001 atccgccaga ctctacagta cacacagcag tgcatccgcg tcttaaatcc ggcattgtct
     6061 cctctgcgcc ggtgactgtg ttataacggt tttaaccttt agttgccaat tttcttcagt
     6121 gacagatttc acgaaaatat aagttacatt a
//
LOCUS       DAZKFU020000260         6149 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZKFU020000260 DAZKFU020000000
VERSION     DAZKFU020000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.262"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2249
                     /locus_tag="ACHOQQ_RS20665"
                     /old_locus_tag="pgaptmp_004133"
     CDS             <1..2249
                     /locus_tag="ACHOQQ_RS20665"
                     /old_locus_tag="pgaptmp_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HGU4472320.1"
                     /translation="NNDKFSLRLRKHLSLAEEEVWQNCIAKLLVALPDISLFRQPLIA
                     ILMPEIPEVAHEIVHRLHQVADVPQLEMLKLVATDPFTLEILENYQYIDVFNTYGASW
                     SATVLREQGIAALARLAPYAEEDNCGDVLKCINHPLAITALIEAADTNKRCYERLIKS
                     AENFPSATIAALAEALVKKDDKRKHTLLVSLLNANPLLVEKLVIGLSSQAANLLQQCQ
                     QLLNSRNEDFANSEILPMVLVSPPWETQNKNNDNSPVGKYPAKIPPLPLFYQPPLWTR
                     PKIKNSSKVLPDSALQHLGEMLRFPQEEALYPGLLQVKDACTADSLAEFAWDLFTAWL
                     TAGAPSKESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIA
                     LMQLNGIAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDF
                     GSRQFTVSFDETLKPFVRDVSGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVA
                     AQQVARLESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAE
                     DNSYSTADDDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSY
                     ALTEAERNASELTRWAGRKCPSGRVMGLANKGWIKGTPQDGGWIGWMIKPLGRWSLIM
                     EIDEGFAVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQLSVLDAITASELI
                     NDIEALFE"
     gene            2259..5891
                     /gene="yehI"
                     /locus_tag="ACHOQQ_RS20670"
                     /old_locus_tag="pgaptmp_004134"
     CDS             2259..5891
                     /gene="yehI"
                     /locus_tag="ACHOQQ_RS20670"
                     /old_locus_tag="pgaptmp_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGU4472321.1"
                     /translation="MDKELPWLADNAQVELKYKKGKTPLSHRNWPGEPVPVITESLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEKSCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQKNRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNNPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLM
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAVQRDLARQWILHWPEHTATALIPL
                     VFTKSSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEHLLKQSPIEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQTVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRHFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHLLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            5952..>6149
                     /gene="yehK"
                     /locus_tag="ACHOQQ_RS20675"
                     /old_locus_tag="pgaptmp_004135"
     CDS             5952..>6149
                     /gene="yehK"
                     /locus_tag="ACHOQQ_RS20675"
                     /old_locus_tag="pgaptmp_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGU4472322.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDA"
BASE COUNT         1625 a         1555 c         1522 g         1447 t
ORIGIN      
        1 ggaataacga caaatttagt ttacgtctgc gtaaacattt atcactagca gaagaagaag
       61 tatggcaaaa ttgcattgca aaattactcg ttgcgttacc agatatatca ctctttcgcc
      121 aaccgttaat tgccatttta atgccagaaa tacccgaagt ggcccatgag atcgtacatc
      181 gtttacacca ggtcgcagat gttccacaac ttgaaatgtt gaaactggta gcgacagatc
      241 cattcacgct cgaaatactg gagaactatc aatacattga tgtatttaac acctatggtg
      301 ccagttggtc agctacggta ttacgggagc aaggtatagc ggcactggct cgcctggctc
      361 cctatgctga agaagataat tgcggtgacg tactgaaatg tattaatcat ccgttggcca
      421 ttacggcact catcgaagcc gccgatacta ataaacgctg ttatgaacga ctaataaaaa
      481 gtgccgaaaa ttttccttct gccacgatag cggcattagc cgaagcattg gtaaaaaaag
      541 atgataaacg caaacatacg ttgctggttt cattacttaa tgcaaatcct ttattggttg
      601 aaaaattagt catcggatta tcgtcgcaag cggcaaattt attgcaacaa tgtcagcaat
      661 tgctgaatag tcgcaatgaa gattttgcaa actcagaaat tctaccaatg gtactagtct
      721 cgccgccctg ggaaactcaa aataaaaata acgacaacag tcctgtgggt aaatacccgg
      781 cgaaaatccc ccctttaccg ttgttttatc aacctccatt atggactcgc ccgaaaatca
      841 aaaatagcag caaggtactg ccagatagcg cactccagca cctcggtgaa atgctccgct
      901 tccctcagga agaggccctg tatccgggat tattgcaggt gaaagatgcc tgtaccgccg
      961 actcactggc tgaatttgcc tgggatctgt ttaccgcctg gttgactgcg ggcgcaccgt
     1021 cgaaagagag ttgggcgttc actgcgttag gcgttcttgg taacgatgac accgcccgca
     1081 aactgacgcc attaatacgc gcctggcctg gtgaatccca gcataaacgc gccaccgttg
     1141 ggttggatat tctcgccgct atcggtagtg atatcgccct gatgcagctt aacggtattg
     1201 cccagaaact gaaattcaaa gcattacagg agcgggcaaa agaaaaaatt gccgacattg
     1261 ccgaaagccg cgaactcacg gtggcagagc ttgaagatcg gttagcaccg gatctcggtc
     1321 tggatgataa cggttcgttg ctgctggatt tcggctcacg ccagttcacc gtcagctttg
     1381 atgaaacctt aaaaccgttt gtgcgtgatg tttctggcag ccgtctgaaa gacctgccca
     1441 aaccaaacaa aagcgatgat gaatcacagg cgaacgatgc tgttaaccgc tacaaattgc
     1501 tgaaaaaaga tgcgcgtacc gtcgccgccc agcaggtagc aaggctggaa tccgccatgt
     1561 gcctgcgccg ccgctggtcg ccggaaaact tccagctctt cctggttgag catccgttgg
     1621 ttcgccactt aacccgccgt ctgatttggg gtgtttatag cgccgaaaac cagctactgg
     1681 cttgctttcg cgtagcggaa gataacagct acagtaccgc tgacgatgat cttttcaccc
     1741 tgccggaagg cgatatctct atcggcattc ctcacgttct ggaaatatca ccaacggatg
     1801 ctgccgcctt tggtcagctt tttgccgact acgaactgct accaccgttc cgtcagcttg
     1861 accgtaacag ttacgccctg acagaagccg agcgcaacgc cagcgaactg acccgctggg
     1921 caggcagaaa atgcccgagc ggtcgggtca tggggctggc gaataaaggc tggataaagg
     1981 gcaccccgca ggatggaggc tggatcggct ggatgatcaa acctctgggc cgctggtcgt
     2041 taatcatgga aatcgatgaa ggttttgcgg taggcatgtc gccagccgaa ctgagtgccg
     2101 agcagctctt aagcaagctg tggctatggg aaggcaaagc agaaagctat ggctggggga
     2161 gtaattcaac acaggaagcg cagctctccg tactcgatgc catcaccgcc agcgagctga
     2221 ttaacgatat tgaagctctg tttgaataag gaaagtgcat ggacaaggaa ttaccgtggc
     2281 tggcggataa cgcccaagtg gaactgaaat ataaaaaagg caaaacacca ctcagccatc
     2341 gcaattggcc gggcgaaccg gtgcctgtta tcactgaaag cctcatccag acactgggtg
     2401 atgaattgct acaaaaagca gagaagaaaa aaaacattgt ctggcgttat gaaaattttt
     2461 cactggagtg gcagtccgcc attacgcagg ccatcaactt gatcggcgaa cacaaaccct
     2521 cagtcccggc ccgcacaatg gcggcgctag cctgtatcgc gcaaaatgac agccaacagt
     2581 tgctcgacga aatcgtccaa caagaggggc tggaatatgc gactgaggtg gtgattgcac
     2641 gccagtttat tgcacggtgt tatgagagtg atcctctggt agtcacattg cagtatcaga
     2701 acgaggacta tggctatggt tatcgctcag aaacctataa cgaattcgat ctccgactgc
     2761 gtaaacacct ctctctggca gagaaaagct gctggcagcg ttgcgccgac aaactcattg
     2821 ccgcactgcc aggaataacc aaagttcgcc gtccttttat tgcgctgatc ctcccggaaa
     2881 agcctgaaat agccaatgaa ctcgccagtc ttgaaagctc ccggtcaagt ctccattcaa
     2941 aggagtggtt aaaggtcgtt gctactgata atacggcggt aaaaaaactc gaacgatact
     3001 ggggcctgga cgtatttagc gatcgcgaag ccagctacat gtcacaaaaa aaccgcttcg
     3061 gctacgcggc ctgcgcctcc cttttgcgcg aacaaggact ggcagccgtt ccgcgcctgg
     3121 caatgtatgc tcataaagaa gattgcggca gtctgctggt acaaattaac catccgcaag
     3181 tcatccgcac cttgctgtta gttgctgata aaaacaaccc cagcctgcaa cgtgtggcta
     3241 aatacagtaa aaacttcccc catgcgacgc tcgccgcact ggcagaactg ctggcgttaa
     3301 aagaaccacc agcccgccct ggttatccaa tcatcgaaga caaaaagctg cctgcacagc
     3361 aaaaagctcg agatgaatac tggcgtacgc tgttacaaac gctgatggca tcgcagccac
     3421 aactggcagc agaagtgatg ccgtggttaa gtactcaagc cagggcagtg ctgaagagtt
     3481 atttatcggc actgccaaaa ccggttattg atggcaccga taacagcaat ctgcctgaaa
     3541 tcctcgtttc accaccgtgg cgtagtaaga aaaaaatgac agctccacgt cttgatttgg
     3601 caccgctcga attaaccccg caagtttact ggcaaccagg cgaacaagag aggcttgcct
     3661 ccactgagtc tgcccgttat ttcagcacgg aatctcttgc ggaacgcatg gaacaaaaaa
     3721 gtgggcgagt tgtattacag gaactgggtt ttggggatga tgtatggctg tttctgaatt
     3781 atatactccc cggaaaactg gatgctgcac gcaattcact cattgttcag tggcattact
     3841 accaggggcg ggttgaagag atcctgaatg gctggaactc cccggaagca caattagcag
     3901 aacaggcgct ccgcagcggt cacatagaag cgttaattaa catatgggaa aatgacaact
     3961 actcacgtta tcgtccggaa aagagtgtct ggaacctgta tttattggca cagttgccgc
     4021 gtgagatggc tttgaccttc tggctgcgta tcaatgagaa aaaacatctg ttcgcgggtg
     4081 aggactattt tctcagtatc ctcgggttgg atgcgctacc aggtctgatg ttggcttttt
     4141 cacatcgtcc aaaagaaaca tttccgttaa ttttaaattt tggcgcaaca gaactggccc
     4201 tgcccgttgc ccgcgtctgg cgacgtttcg cggtgcagcg tgatctggct cgccagtgga
     4261 ttttacattg gccggaacat acggctactg cacttatccc tcttgtcttc accaaatcca
     4321 gcgataatag cgaagctgca ttacttgccc tgcgtttact ttacgaacag ggacatggtg
     4381 aattgctgca aaccgtggca aaccgctggc agcgtacaga tgtatggcct gccctggagc
     4441 atttacttaa acagagtcca atagagattt acccggcacg cattccaaaa gcccctgatt
     4501 tctggcatcc gcaaatgtgg tccaggccgc gccttatcac taataatcaa actgttaccg
     4561 atgacgctct ggaaattatc ggcgaaatgc tgcgctttac ccagggggga cgtttttata
     4621 gcgggctgga acaactgaaa acgttctgcc agccacaaac gctggcagct tttgcctggg
     4681 atctcttcac tgcgtggcaa caagctggtg cccccgcaaa agacaactgg gcatttctgg
     4741 cgttaagtct ctttggtgac gaaagcacgg cacgggatct gacgacacag atcctcgcct
     4801 ggccacaaga aggcaaatct gcccgtgctg tcagcggcct gaacatcctt accctgatga
     4861 ataatgatat ggcgctgata cagctgcatc atatatcgca acgggctaaa tcccgcccct
     4921 tacgtgataa cgcggcggaa tttcttcagg tggtcgcaga aaatcgcggg ctaagccagg
     4981 aagagctagc ggacagatta gtcccaaccc tgggccttga tgatccgcag gcgttgagtt
     5041 ttgattttgg tccccggcat tttaccgttc gcttcgatga aaaccttaac ccggttatct
     5101 ttgatcagca aaacgttcgc cagaaaagcg ttccccggtt gcgcgccgat gacgatcaac
     5161 tgaaagcgcc cgaggcactg gcccgactaa aagggctaaa aaaagatgcc actcaggtga
     5221 gcaaaaacct gctcccgcgt cttgaaacgg ccctacgtac cactcgacgc tggtcgctgg
     5281 cagattttca ttctctgttt gttaatcatc cctttacccg tctggttacc cagcgattaa
     5341 tatggggggt ttatccggca aatgaaccgc gttgtttact caacgccttt cgtgtggccg
     5401 cagaggggga gttctgcaat gcgcaagatg agccaattga cctgcctgcg gacgctctga
     5461 ttggcattgc ccacctgtta gaaatgacag tagaaatgcg cagtgaattt gcacagcttt
     5521 ttgccgatta cgaaattatg ccgccttttc gccagttgtc gcggcgcacg gtgctgctca
     5581 cacctgacga gtcaaccagt aacagcctga ctcgctggga aggtaaatcc gctaccgttg
     5641 ggcaacttat gggaatgcga tacaaaggct gggagtcagg ctatgaggac gcatttgtct
     5701 atgacctggg tgagtaccgg ctggtcctta agttttcacc cggttttaac cactacaatg
     5761 ttgatagcaa agcgctaatg agcttccgtt ctcttcgagt gtaccgtgac aataaatccg
     5821 tcacttttgc cgaacttgat gtgtttgatt tgagtgaggc gttaagcgca cctgacgtca
     5881 ttttccatta aaacacagcg ggcagtgatg caactgcccg ttatcaacac gacttaccag
     5941 cggggaaagc gatgatcgtg caaaaagagc tagttgctat ttacgattat gaggtccctg
     6001 tacctgaaga tccgttttcc ttcagacttg agatccataa atgctctgaa ttatttacag
     6061 gttccgtcta tcgactggag cgattccggc tacatccaac atttcatcaa cgtgatcgag
     6121 aagatgctga cccgctaata aatgatgcg
//
LOCUS       DAZKFU020000261         6141 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZKFU020000261 DAZKFU020000000
VERSION     DAZKFU020000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.263"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..308
                     /locus_tag="ACHOQQ_RS20680"
                     /old_locus_tag="pgaptmp_004136"
     CDS             93..308
                     /locus_tag="ACHOQQ_RS20680"
                     /old_locus_tag="pgaptmp_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4472323.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            721..912
                     /locus_tag="ACHOQQ_RS20685"
                     /old_locus_tag="pgaptmp_004137"
     CDS             721..912
                     /locus_tag="ACHOQQ_RS20685"
                     /old_locus_tag="pgaptmp_004137"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4472324.1"
                     /translation="MCKRLLAIPGFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1054..2073
                     /locus_tag="ACHOQQ_RS20690"
                     /old_locus_tag="pgaptmp_004138"
     CDS             1054..2073
                     /locus_tag="ACHOQQ_RS20690"
                     /old_locus_tag="pgaptmp_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGU4472325.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWVRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKIRLLGISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2830..3009
                     /locus_tag="ACHOQQ_RS20695"
                     /old_locus_tag="pgaptmp_004139"
                     /pseudo
     CDS             2830..3009
                     /locus_tag="ACHOQQ_RS20695"
                     /old_locus_tag="pgaptmp_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3184..5154
                     /locus_tag="ACHOQQ_RS20700"
                     /old_locus_tag="pgaptmp_004140"
     CDS             3184..5154
                     /locus_tag="ACHOQQ_RS20700"
                     /old_locus_tag="pgaptmp_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HGU4472326.1"
                     /translation="MYMNVIRTVICTLIILPVGLQTATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLEPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNVASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYAPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            5173..5964
                     /locus_tag="ACHOQQ_RS20705"
                     /old_locus_tag="pgaptmp_004141"
     CDS             5173..5964
                     /locus_tag="ACHOQQ_RS20705"
                     /old_locus_tag="pgaptmp_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HGU4472327.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1818 a         1370 c         1497 g         1456 t
ORIGIN      
        1 gtaagcgatg agatggtctt ccccctcctt cacgacaacc tttaattacg tcatgatgga
       61 taaactttaa ccacaaaagt aagagggata ccatgtctca acagaaaatc accggcatcg
      121 atctggctaa aacgaacttt tatcttttca gtatcaatgc tcatggcaaa cccgcaggaa
      181 ggatcaaact ttctcgcaat caactgctta actggctggt gcaacagccc aaaatgactg
      241 ttgcgatgga ggctcgcggg catcccatca ctgggccagg gaaatcagga aactcgacca
      301 tgatgtaata tttcttccgg cgcaacatgt taaagcttat cagcgacgcc agaaaaatga
      361 ttatgacgac gctcaggcaa tagcagaggc ctgccaacat ggaactatac gccccgtacc
      421 aataaaaact ctggagtaac aggacgtcca gacttttttg aaaatgcgcc ggttggtttc
      481 gatggaaagg acacaactga taaatcacgt tcgtggactt cttgctgaat atggcattgt
      541 gttcagtaaa ggcgcaacag aattacgcca aaaattaccg gcattacttg aggatgcgga
      601 aaacgaactc actgatacca tgaggacgtt attgcatcgg cagtatatac ggttgattac
      661 actagataat gaactggagt ggtatgattc tgaactgaaa aatatgtccg ccaagatcct
      721 atgtgcaaac gcttgttagc cattcctgga ttcagccctc tggttagtca ggcaataaaa
      781 agctggatgg gggatggaaa acagtttaga cgaggaagag atgcatctgc gactctggga
      841 ctggttcccc gacagttcag cacaggtggg aagcaggttc ttctgggtat aacaaaattt
      901 ggagaaatgt aatgaacaca tcccacccca ttaagtcagg tgctctgtga tggatgaagg
      961 gaaatactgg aaggtgtgga gcctcgcaga tttccggcat catagtgccc aatacaggat
     1021 gcataaagct ccctcagact ggagactcca caaatgaaat atacaccggt tggcgttgat
     1081 atcgcaaaac atctgattca ggttcatttc atcgacgagc acacaggtga agtgattgat
     1141 aagcaactac gtcgtcagga ttttctgatg ttcttctgta atcgggaacc ctgcctgatt
     1201 ggtatggagg cctgtagagg ctctcagcac tgggtgcgtg aactgacaaa gctggggcat
     1261 aaagtccggt tgttacaggc ccactttgtt aaggcattcg tcatgggaaa taagaatgat
     1321 gtgatggatg cccgggctat ctggatggct gttcagcagc caggtaagga catcgcggtt
     1381 aaaacagaag aacaacagtc agtgctggtt ttgcaccgtt cccgcagaca attggttaag
     1441 ttccgcacca tgcagattaa ttctctgcac ggcacgctcc tagaatttgg taaaaccatt
     1501 cacaaaggtc gggcagcgat ggagcgagag ctccctgaag cactggagcg gatgaaagag
     1561 agactgccgc cgtatctcat tacagtgctg gaaaaccagt acaaccggct gaatgagctg
     1621 gactcgctga tagaggatat tgaaaaaaag cttaccagcg tggcgagaca gaatgaaacc
     1681 tgtaagcggt tgctggatat tcccggcgtt gggccactta ttgcaacggc agcggtggcc
     1741 accatggggg aagcatcagc gtttaaatca gggcgagagt tcgcggctta tgttggtctg
     1801 gtgcccaaac agactggctc aggagggaaa atccgcctgc tggggataag caaacgtggt
     1861 aatacatatc tgcgagcctt gtttattcat agcgcaaggg cggcagcatt actgcccaaa
     1921 gaacctggtc cgtggataac cgaactgaaa aaacgtcgtc caaccagcgt ggcaatcgtc
     1981 gccatggcaa acaaactggc acgaacagta tgggcgatag cagctcatgg aagtaaatac
     2041 gacaagaatc atgtcagtat cagatcatat taatctctga taccgataaa tattaacttt
     2101 taatgaaagg atgaatactg aaaggtaact ctggcggcca tagtgatgac aacgacaggt
     2161 aagaccggga ctcactaaac ctgaatattg tgttgagcta aaagctcgct ggagaaatga
     2221 ggtgtgagtc agcgaattac ataggggctc gcagcttcat ggctgcaaaa aagccggata
     2281 taaagctgca acctacccgt cactgtcaag gcaatggatg ccttgcaacg ggatgtgttc
     2341 atataatcaa tatgtcagat ctatgctgat acagggtgcc agagcggtat tattcagagc
     2401 ctcagagaaa actgattcac ttcgtgtgtg ggttaattgt attcgggaaa agcgtggctt
     2461 caaccgggca gttgttgctc ttgcaaataa actccttcgg atcgcctggg caattatcgc
     2521 ccgcaatgag atgtattttc ccgcagcaaa acaaaatcaa taatctacgt aaaatcagta
     2581 ggagtaaagc ttaccctgaa attgcaaaga atgcagaatg atgaaaaaca ggtcagaccg
     2641 acgttacgaa aacctgcaag atgagccagg ccagtaaggc cgagtaagcg ataaggtcgg
     2701 agcgcgcgaa tgtctcatca aggccagaag caataacttc atgaacaaac cggatatacg
     2761 aaagtaaacc tgtccctatc atagttgctg tactcttgca atacggggga agatcatctc
     2821 atcacttacg aacgattcgt gaaaacgatg aagaaagact acatcgcgtg catgccgaaa
     2881 tctaatgtaa gaacggcatt gcataatctt gcagtggaga tcgggtatta caatgaaaac
     2941 catccgcata gtgcgttagg ttatctctca ccaagggaat atcgacgtca gcaagtaacg
     3001 tcaccttaga tgcagtagtt gtcaggaaat agtgactcaa gggcaggctt accatataca
     3061 ctcttagtgg ttcgactggg tattagaata gctattaaca gattagcact gaacactgcg
     3121 taatagtggt tgcacaaaca aaatgttatg ttataacatt gctgtattga catatatttt
     3181 cgaatgtata tgaacgtaat cagaactgtc atttgtacat taatcatact tccggtgggg
     3241 ctacagacag cgacaagtca ttcttctatg gctaaagata caatcaccgt tgtcgcgaca
     3301 ggaaatcaga acacggtatt tgaaacgccg tcgatggtca gtgtcatcac gaatgacacg
     3361 ccatggagtc agaatgcggt cacatcagcc ggtatgctga aaggtgttgc gggtctcagc
     3421 cagactggtg caggacggac caatgggcag acctttaatc tacgcggcta tgacaaaagc
     3481 ggggtacttg ttcttgttga cggcgtccgt caactcagtg acatggcaaa aagcagtggc
     3541 acttatctcg agccggcact catcaaacgc atcgaagttg tccgcggacc aaactccagt
     3601 ctgtacggca gtggcgggct ggggggggtg gtggacttca gaactgccga tgctgcagat
     3661 tttcttcccc ccggagagac aaacggtgta agtctgtggg gaaatgtcgc cagtggtgac
     3721 cacagcacag gctcggggct cacctggttc ggtaaaaccg gaaaaacaga tgcgcttctt
     3781 tctgtcatta tgcgtaaaag aggaaatatc tatcagagtg atggtgagcg tgcacctaac
     3841 aaggaaaaac ctgcagccct gtttgcgaaa ggctctgtca gtataacaga cagtaacaaa
     3901 gcaggtgcca gcttgcgtct ctaccagaat aacaccactg aaccgggcaa tcccactctg
     3961 acacatggtg acaacggcct gcgtgacaga aaaacagcac aaaatgacgt gcagttctgg
     4021 taccagtacg cccctgcgga taacagcctc atcaatgtaa agtcaacgtt atatctcagt
     4081 gatatcacta tcaagacaaa cggtcacaac aaaacagcag aatggcgaaa caacagaacc
     4141 tccggtgtta atgttgtcaa caggagccat actctgattt ttccgggagc ccatcagtta
     4201 agttatggcg ctgaatatta ccgtcagcag cagaagccgg aaggctctgc cacactatat
     4261 ccggaaggaa acattgactt tacatcgttg tatttccagg atgaaatgac aatgaaaagc
     4321 aacccggtta acattatcgt cggttcccgt tatgaccggt acaagagctt caatccccga
     4381 gccggagaac tgaaagccga acgcctgtcc cccagggcgg cgatttcagt ctcaccgaca
     4441 gactggctga tgatgtacgg ctccatatcc tctgcattcc gtgcccccac aatggcagaa
     4501 atgtacaggg atgatgtaca tttttaccgc aagggtaaac ccaattactg ggtacctaac
     4561 cttaatctga aaccagaaaa taacatcacc cgtgagattg gcgcaggtat tcaactggac
     4621 ggcctgctta cagacaatga tcggctgcag ttgaaaggcg gatatttcgg aacggatgcc
     4681 agaaactata ttgccacgcg cgtggatatg aaacggatgc gttcttattc ttataatgta
     4741 tcccgggccc gtatctgggg atgggatatg cagggtaatt accagtctga ttattttgac
     4801 tggatgcttt cttataaccg cacggaaagt atggatgcca gtagcaggga atggctgggc
     4861 tccggcaatc ctgacacact catcagtgac atcagtatac ctgttggtca tggaggagtt
     4921 tctgccggat ggcgcgctga actttcggca gcagccacgc atgtgaaaaa aggcgatccc
     4981 catcaggctg gctatgccgt acattccttt tcactgtctt ataagcctgt aagtgttaaa
     5041 ggctttgagg catcagtgac tctggataat gccttcaaca agcttgccat gaatggcaaa
     5101 ggtgtgccgc tttcaggcag aactgtcagt ctttataccc gttatcagtg gtaacctcgc
     5161 aggaaataaa tcatgatgaa aaatacaggc tatatcttag ctctttgtct gacagcatcg
     5221 gagcatgccc tggcccacga tgtctggatt accggtaaac aggcagagaa caacgttacc
     5281 gcagagattg gttatggtca taatttcccc tcaaagggca caattcctga cagaaggaat
     5341 ttctttgaaa atccccggct ttataacggg aaagagataa taacgctgaa gccaacgtcc
     5401 acggattatg tctataaaac tgagtctgca ggcaacggta atagctacgt tctgtcaacg
     5461 aaaatgaaac cgggatactg gtcgagaacc tcgtcaggat ggaagcctgt cagtcgggag
     5521 ggcagaaatg atgtggcata ctgtgagttt gtgactaaat atgcaaaatc ttttattcct
     5581 ggtgaacagc agatgccagc acaactctat cagtctccaa caggggatga gcttgaaatc
     5641 attccattat ccgatatcag tcgtttcggt gaagatgtga agctgaaagt tctgtataaa
     5701 acatccccgc ttgccggagc tactctggag cttgactctg tcagttatct gaaatcatcc
     5761 cgtcataccc atgcagctga gcataaacat tcggctcata aagcagaact tacctttgta
     5821 agtaatgagg acggtatcat cactgtacct tcccttcatg tcgggcaatg gctggctaaa
     5881 gtcaaaaata agaaagtatt tccggacaag agtctgtgtg atgaaactgt cgatgtggca
     5941 accttaagct tctcccgaaa ttaataatac tattcctgcc ctgatttatg ggcagggtat
     6001 tatcaatacg agaatagcct accgacatcc gtgtaaaaaa ccacataatg atttttcata
     6061 tccatttcca gatatactta tgtaaagatc acctccaaaa aagcgtaata gtaataaaaa
     6121 tccctgaagc accctgttca a
//
LOCUS       DAZKFU020000262         6136 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZKFU020000262 DAZKFU020000000
VERSION     DAZKFU020000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.264"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            275..1933
                     /locus_tag="ACHOQQ_RS20710"
                     /old_locus_tag="pgaptmp_004142"
     CDS             275..1933
                     /locus_tag="ACHOQQ_RS20710"
                     /old_locus_tag="pgaptmp_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGU4472328.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CADGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1930..2886)
                     /locus_tag="ACHOQQ_RS20715"
                     /old_locus_tag="pgaptmp_004143"
     CDS             complement(1930..2886)
                     /locus_tag="ACHOQQ_RS20715"
                     /old_locus_tag="pgaptmp_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGU4472329.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3037..4152
                     /gene="macA"
                     /locus_tag="ACHOQQ_RS20720"
                     /old_locus_tag="pgaptmp_004144"
     CDS             3037..4152
                     /gene="macA"
                     /locus_tag="ACHOQQ_RS20720"
                     /old_locus_tag="pgaptmp_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGU4472330.1"
                     /translation="MKKRKTVKKRYVIALVIAIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4149..6095
                     /gene="macB"
                     /locus_tag="ACHOQQ_RS20725"
                     /old_locus_tag="pgaptmp_004145"
     CDS             4149..6095
                     /gene="macB"
                     /locus_tag="ACHOQQ_RS20725"
                     /old_locus_tag="pgaptmp_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGU4472331.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTVPVVNMASGWRQFASGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
BASE COUNT         1426 a         1473 c         1708 g         1529 t
ORIGIN      
        1 caacagcggg ttgccccata tagtcatttg tctgattgac agtgtagtgc aagcaaaaga
       61 tttaatcctt taggcgtaat aaaaaataat ttatcatgct aattatttga ttttgttgtt
      121 tttgcagact tatcagcaag agggagtata acgcgattat tcgctcattt ttcagacatt
      181 tgccatgctt aaatgtgatg tcatcacgta ttagcaaggc ctttcccgtt atactgccag
      241 cgtaaaggat aagtcacata tttctggagg ggatatgatt cttgagcgcg ttgaaattgt
      301 gggttttcgc ggtatcaacc gtttgtcgtt gatgctggaa caaaacaacg tcctgattgg
      361 ggagaacgcg tggggtaaat ccagcttgct ggacgcctta accctgctgc tatcgccaga
      421 atcagatctc taccattttg agcgcgacga tttctggttc ccgccgggag atatcaacgg
      481 gcgagaacat catctgcata ttattttgac cttccgcgaa tcgctgccag gccgacatcg
      541 ggttcgccgt tatcggccgc tggaagcgtg ctggacgcca tgcgccgatg gctatcaccg
      601 tattttttat cgtctggaag gggagagtgc ggaagacggc agcgtgatga cactgcgcag
      661 ttttctcgat aaagacggac atccgattga tgttgaggat attaacgatc aggcacgcca
      721 tctggtgcgt ttaatgccgg tgctgcgctt gcgtgatgcc cgttttatgc gccgtattcg
      781 taacggcacg gtgccaaatg tccccaatgt ggaagtcacc gcgcgccagc tcgatttcct
      841 cgcccgtgag ttgtcctcac atccgcaaaa tctctctgat ggacagattc gtcagggact
      901 ttccgcaatg gtgcagctgc ttgagcatta tttctctgag cagggggctg gacaggcgcg
      961 atatcgttta atgcggcggc gggccagcaa tgagcaacga agctggcgct atctggacat
     1021 catcaaccgg atgattgacc gacctggagg gcgctcgtat cgggttattt tgctcggcct
     1081 gtttgctact ttattgcagg caaaaggcac attgcgactg gataaagacg cccgtccatt
     1141 gttgctgatc gaagatccag aaacgcgttt acaccccatt atgctttcag ttgcctggca
     1201 tctgttgaat cttttgcctt tacaacgtat tgctaccacc aactctggcg agctgctttc
     1261 gttaactcca gtggaacatg tttgccgcct ggtgcgtgaa tcttctcgtg ttgccgcctg
     1321 gcgtctgggg ccaagtggct tgagcaccga agacagccgc cgcatctctt ttcacattcg
     1381 ttttaatcgt ccgtcatcgc tgtttgctcg ctgctggttg ctggtggaag gggaaacgga
     1441 aacctgggtt atcaatgaac tggcgcgtca gtgcggacat cattttgatg ccgaagggat
     1501 caaggtcatt gagtttgccc agtccgggct aaagccactg gttaaatttg cccgccgaat
     1561 ggggattgaa tggcatgtgc tggtcgatgg cgatgaagca gggaagaaat atgccgctac
     1621 ggtacgcagc ctgttgaata atgatcggga agccgaacga gaacatttaa cggcgttacc
     1681 ggcgctggat atggaacatt ttatgtatcg ccagggattt tccgatgtgt tccaccgcgt
     1741 ggcgcaaatc ccggaaaatg taccgatgaa tctgcgcaaa attatctcga aagcgatcca
     1801 tcgctcttcc aaacccgatc ttgccattga agtggcaatg gaggccggac gtcgtggtgt
     1861 ggactccgta ccgacgctgc tgaaaaaaat gttctcacgc gtgctgtggc tggcgcgcgg
     1921 tcgcgcggat taaccgcgaa acatcgtggc catttgtggc tgaatagcgt cgagcatctc
     1981 atagcgccga cggtattcag cccgtttttt actggcgatt tcggcaatct cttttcgtgc
     2041 tatctgtgct ggaaggcggt aatggcgttc agcatcacat acgccgccaa ccgattccca
     2101 gaaagcgttg taatcggcat ggatcttgcc ttctttatcg cgataacgca ggctgcggta
     2161 aatatgcgtt tcattgctga cggcaataat ctgctctacc tgcaaacgtt gggcaaacag
     2221 acaggccgct tccatcacga ggcgtttggg aaatagcccg tggcaggctt tcgtcgcatt
     2281 ctggatttcc tgatgtggga tttcccattt tgcgccttgc agtccgccaa taaacatcgt
     2341 tcttttcccc tgatattcac acagggtaaa cgtgatctct gccagaggaa taccttcgct
     2401 gttgcggaac aggattgtgc tgtcaccttc tttatccatt gagatcatca tggtcagctc
     2461 aagcgtgaac tgctcgccgt ttttgccttc cagcttcgcc agttgcagcc cgggggtatt
     2521 caaatataag ctgaattctt ccgccgacat acacccgcgg agtaacgcat aatggtaacg
     2581 taacgcctcc agcaattgct tacggctaag attcgccgca aggtaagggc gatgcagacg
     2641 tacaggcagt cgcggctggc gcgttaacaa tacattgaga ttaggccagt gggaaagttc
     2701 gttcatccac tcaacgctta aacgcggcat aatcaacgag cgcagcaaaa atttctggcg
     2761 aaaactacgg cgatgccaga atttacccgg ccgacactgt ccacgtgcca gactaagaaa
     2821 aagtgacagg ctgctgagag attcagatgg cgtaaaggtc cgttcagtta gctgcgacat
     2881 attcatgaaa tcaatggtta tacatgacgt cgatttcacc attgcgtatc ttaaccaaac
     2941 atcaatagtg tgattactaa cgtaaatttt agggttttgt tgatatttcg ttgaagttaa
     3001 tgacccggat tggcatatgg agtattcaga atatttatga aaaagcggaa aaccgtgaag
     3061 aagcgttacg ttattgcgct ggtgatagcc atcgccggac tgattacgtt atggagaatt
     3121 cttaacgcac ccgtgccgac ttatcagaca ctgattgtgc gccccggtga tttacagcaa
     3181 agcgtgctgg cgaccggaaa gctggacgcg ctgcgtaagg ttgatgtggg cgcgcaggtc
     3241 agcggtcagt tgaaaacact gtcggtggcg attggcgata aagtaaaaaa agaccagctt
     3301 ttaggggtca ttgatcctga acaggctgaa aaccagatca aggaggtcga agcaacgctg
     3361 atggagctac gcgcgcagcg gcagcaggcg gaagcggagc tgaaactggc gcgggtgacg
     3421 tattcccgtc agcaacgtct ggcacaaacg caggcggttt cacagcagga tctcgacacc
     3481 gccgcgacgg agatggctgt gaaacaggcg caaattggca ccattgacgc gcaaatcaag
     3541 cgcaatcagg cttctctcga tacggctaaa accaatctcg attacactcg catcgttgcc
     3601 ccgatggccg gggaagtcac gcaaatcacc actctgcaag gccagacggt gattgccgca
     3661 caacaagcac cgaacattct gacgctggca gatatgagca ccatgctggt aaaagcgcag
     3721 gtttctgaag cggatgtaat ccacctgaag ccggggcaaa aagcctggtt tacggtactt
     3781 ggcgatccac tgacgcgcta cgaggggcaa atcaaggatg tactaccgac gccggaaaag
     3841 gttaacgacg ctattttcta ttacgcccgt tttgaagtcc ccaaccccaa tggtttactg
     3901 cggctggata tgactgcgca agtgcatatt cagctcaccg atgtgaaaaa tgtgctgacg
     3961 atccctctgt cggcgttagg cgatccggtt ggcgataatc gttataaagt caaattgttg
     4021 cgtaatggtg aaacacgcga gcgtgaagtg acgattggcg cacgtaacga taccgatgtt
     4081 gagattgtca aagggctgga agcgggcgat gaagtggtga ttggtgaggc caaaccagga
     4141 gctgcacaat gacgcctttg ctcgaattaa aggatattcg tcgcagctat cctgccggtg
     4201 atgagcaggt tgaggtgttg aagggcatca ccctcgatat ttatgctggt gagatggtgg
     4261 cgattgttgg cgcttcgggt tccggtaaat cgaccctgat gaatattctc ggctgtctgg
     4321 ataaggccac cagcggcacc tatcgcgtcg ccggtcagga tgttgccacg ctggacgccg
     4381 atgcgctggc gcaactgcgc cgcgagcatt tcggctttat tttccagcgt taccatttgc
     4441 tttcgcattt aaccgccgag cagaacgttg aagtacccgc cgtctatgct ggtcttgagc
     4501 ggaaacagcg gttgcttcgc gcccaggagt tgctgcaacg actggggctg gaagaccgta
     4561 cagagtatta tccggcacag ctttcgggtg gtcagcaaca gcgcgtcagc atcgcgcggg
     4621 cattgatgaa cggcggtcag gtaattcttg ccgatgaacc gaccggcgca ctggacagcc
     4681 attctggcga agaggtgatg gcgatcctgc atcagctgcg cgaccgcggg catacagtga
     4741 ttatcgtcac ccacgatccg caggtcgctg ctcaggccga gcgggtgatc gaaattcgcg
     4801 acggcgaaat tgtgcgcaat cctcccgcca ttgaaaaagt gaatgttgct ggcgggacgg
     4861 tacctgttgt caacatggcg tctggctggc gtcagtttgc cagcggtttt aacgaggcgc
     4921 tgacgatggc atggcgggcg ctggcagcga ataaaatgcg tactttactg accatgctgg
     4981 ggattattat cggtattgcg tcggtggttt ccattgtcgt agtaggtgac gccgccaaac
     5041 aaatggtgct ggcggatatt cgttctattg gtacgaatac tattgacgtc taccccggga
     5101 aagattttgg cgatgacgat ccgcaatatc agcaggcgct gaagtacgac gacttaatcg
     5161 ccatccaaaa acaaccgtgg gtcgcttcag ccacacctgc cgtctcgcaa aacctgcgcc
     5221 tgcgttacaa taatgttgat gttgctgcca gtgccaatgg cgtgagcggc gattatttta
     5281 atgtctatgg catgaccttc agtgaaggaa acacctttaa ccaggaacag ctgaacggtc
     5341 gtgcgcaggt cgtggttctc gacagtaata ctcgccgcca gcttttcccc cataaagcag
     5401 atgtggttgg cgaggtgatt ctggtcggca atatgcccgc cagagtcatt ggtgtggcgg
     5461 aagaaaaaca gtcgatgttt ggtagcagta aagtgctgcg tgtctggcta ccttacagca
     5521 cgatgtccgg gcgagttatg ggccagtcgt ggctgaactc cattactgtc agggtgaaag
     5581 aaggatttga cagcgccgag gcggaacagc aactcacgcg tttactttca ctgcgccacg
     5641 gtaagaagga tttctttacc tggaacatgg acggcgtctt gaaaaccgtt gaaaagacca
     5701 cacgtacttt acaactgttt ctgacgctgg tggcggtgat ttcgctggtg gtgggcggta
     5761 ttggtgtaat gaatattatg ctggtgtcag tgaccgagcg gacgcgggaa attggcattc
     5821 gcatggctgt aggtgcgcga gcaagcgatg ttttgcaaca gttcctgatc gaagccgtac
     5881 tggtttgcct ggtcggtggc gcgttgggaa taacattgtc actgttaatt gctttcacct
     5941 tgcagctttt cttacccggt tgggagattg gtttttcacc gttggcactg ctgctggcgt
     6001 ttctctgctc gacggccacc gggattttat ttggctggtt acccgcacga aatgcggcac
     6061 gactggatcc agtagatgct ctggcacgag agtaattttt gagataaaaa tgccagccga
     6121 tcgggctggc attttg
//
LOCUS       DAZKFU020000263         6126 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZKFU020000263 DAZKFU020000000
VERSION     DAZKFU020000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.265"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1440
                     /gene="ydfI"
                     /locus_tag="ACHOQQ_RS20730"
                     /old_locus_tag="pgaptmp_004146"
     CDS             <1..1440
                     /gene="ydfI"
                     /locus_tag="ACHOQQ_RS20730"
                     /old_locus_tag="pgaptmp_004146"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU4472332.1"
                     /translation="AKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSD
                     WGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLETVL
                     AAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALA
                     RRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRI
                     VPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLP
                     YEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYALMLQEQAPTLKVQGV
                     DLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGV
                     AGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDN
                     SLFTAKVTEAYLFLLAHGAKATVAKYSVK"
     gene            complement(1475..1678)
                     /gene="ydfZ"
                     /locus_tag="ACHOQQ_RS20735"
                     /old_locus_tag="pgaptmp_004147"
     CDS             complement(1475..1678)
                     /gene="ydfZ"
                     /locus_tag="ACHOQQ_RS20735"
                     /old_locus_tag="pgaptmp_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGU4472333.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1855..2541)
                     /gene="rspR"
                     /locus_tag="ACHOQQ_RS20740"
                     /old_locus_tag="pgaptmp_004148"
                     /gene_synonym="ydfH"
     CDS             complement(1855..2541)
                     /gene="rspR"
                     /locus_tag="ACHOQQ_RS20740"
                     /old_locus_tag="pgaptmp_004148"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGU4472334.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2630..3376)
                     /gene="ydfG"
                     /locus_tag="ACHOQQ_RS20745"
                     /old_locus_tag="pgaptmp_004149"
     CDS             complement(2630..3376)
                     /gene="ydfG"
                     /locus_tag="ACHOQQ_RS20745"
                     /old_locus_tag="pgaptmp_004149"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGU4472335.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3513..5558
                     /gene="dcp"
                     /locus_tag="ACHOQQ_RS20750"
                     /old_locus_tag="pgaptmp_004150"
     CDS             3513..5558
                     /gene="dcp"
                     /locus_tag="ACHOQQ_RS20750"
                     /old_locus_tag="pgaptmp_004150"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGU4472336.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMLQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGSLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWGFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDEVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5603..6121)
                     /gene="ydeJ"
                     /locus_tag="ACHOQQ_RS20755"
                     /old_locus_tag="pgaptmp_004151"
     CDS             complement(5603..6121)
                     /gene="ydeJ"
                     /locus_tag="ACHOQQ_RS20755"
                     /old_locus_tag="pgaptmp_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGU4472337.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1509 a         1480 c         1583 g         1554 t
ORIGIN      
        1 gcaaaagcga cgctccctgt ttatgatcgt aataacttgg ccccaagaat tgttcattta
       61 ggctttggtg catttcaccg tgcgcatcag ggcgtgtatg ccgatattct tgctacagaa
      121 catttcagtg actggggata ttatgaggtc aacttaatcg gtggcgaaca gcaaattgcc
      181 gatttacaac agcaagataa tctttatacc gttgcggaaa tgtcggccga tgcgtggacg
      241 gcacgcgtcg ttggcgtcgt taaaaaagcc ttgcacgtac agatggatgg cttagaaacc
      301 gtgttggctg cgatgtgtga accgcaaatc gcgattgtct ctctgacaat caccgaaaaa
      361 gggtatttcc actctccggc gaccggacag ttaatgctcg atcacccgat ggtcgctgcc
      421 gacgtgcaaa atccccacca gccgaaaaca gcaacagggg tgattgttga ggcgctggct
      481 cgccgtaaag cggcaggact tcccgcattt accgtcatgt catgtgacaa catgccagaa
      541 aacggtcatg ttatgcgtga cgttgtcact tcatacgcac aagccgttga tgtaaaactg
      601 gcacaatgga tcgaagataa cgtgactttc ccatcaacaa tggtggaccg tattgtgccc
      661 gcagtgacag aggatacgct agcgaaaatc gaacagctta ccggtgtgcg cgatcctgct
      721 ggcgttgcct gtgaaccttt ccgccagtgg gtaatcgaag ataactttgt tgccggacgt
      781 ccggaatggg aaaaagcggg agccgaactg gttagtgatg tgctgcctta tgaagagatg
      841 aagttgcgca tgctcaacgg cagtcattca ttcctggcgt atttgggtta tcttgccgga
      901 tatcagcaca ttaatgactg tatggaagat gaacattatc gttatgcggc gtatgccttg
      961 atgttgcagg aacaagcgcc gacgctgaaa gtgcagggcg ttgatttgca agattacgct
     1021 aaccgattaa ttgcacgcta tagcaacccg gcgttacgcc atcgaacctg gcagattgcg
     1081 atggatggca gccagaaatt gccacagcgg atgttggatt ctgttcgctg gcatctggcg
     1141 catgacagca agttcgatct gctggcgctg ggcgtcgcgg gttggatgcg ttatgtcggt
     1201 ggtgttgatg aacagggaaa tccgattgaa atcagtgacc cactgttacc tgttattcag
     1261 aaggctgtac aaagtagtgc cgaagggaaa gcgcgcgtcc agtcattgct ggcgattaag
     1321 gcgatctttg gtgatgattt gccagacaat agcttgttta ctgcaaaagt gacggaagcg
     1381 tacttgtttt tattagcgca tggtgcgaaa gcgaccgtgg cgaaatattc cgtgaagtaa
     1441 aaaagcaatg ccgccatcgg cggcattcac acgcttagtt catgccgaga cgaatcaggt
     1501 ccagtggtgc cagtttctct tcacaacctt caacaactac ggtttttccg cggcggattt
     1561 gctcagcggt cagaccttca gtatcaatcg acaggatctt gccagtgtga ccggtgccgc
     1621 taaccataac acggctgcca gtggtgattg cgttacggtt acgatcgtag gtcgtcatag
     1681 gtgttttctc ctttctgatt tacagtcgaa gcccacgtaa cgggcgcaat ataaatactc
     1741 ctgtcgagtg aaatattttt tgtttttgat caagctcaca tcttttttga tgcatgcatg
     1801 agccataagg cgggggaaaa gactctcacc ccggatggga tgagaggaaa tgaattactc
     1861 ttcgctaaac cagtcgctgt tttcctggcg gatttggcgc acggattcgc tgatttcctg
     1921 caaatgttgc gtcattgcac gttctaccgc atcgccatca cgtttttgca gggcagagaa
     1981 aatatcaaga tgctggcgta acagcatttc tggtggagaa acgtggtcga aactcatata
     2041 gcgcacgcga tcaacggtcg ctttcaggtt ctcaatggta tcccacgcca gttgacagtc
     2101 ggcaatctgc gtcaggagtt gatggaagtt gtcatcaagt tcaaaaaaat catccagttg
     2161 cttgcgctca atggcaatgc gttgctggtg aagattttgt tccagttggt agcactggct
     2221 ttcggtaatc atgctcgccg cccgacgcgc caccgcgcac tcaatggcct gacggataaa
     2281 actgccgttg cgcacctggg ccatggaaat tttgttgacg tagctgccac gctgcggacg
     2341 aatttgaatc aggccgtttt ccgccagttt aataaaggct tcacgaaccg gctggcgtga
     2401 cacattgaaa cgaacagaaa cttctttttc cgacaacggt gtgcctgggg caatcaggca
     2461 atggacaatg tcgcgacgaa ggatacgata aatctgctga ttgacaggct gtgtgggatt
     2521 aagttgcgtt tcgacggtca ttcgttcttg cttgtgagtg agttaactgc atgagtctac
     2581 cacttttttg tgacaagcaa taacccggca gttacgccgg gtataaaaat tactgacggt
     2641 ggacattcag tccggcatag ctttgggtaa ccggcatcat ttccagggta ttgatattga
     2701 cgtgagcagg cagcgttgac acccaccaga cggcttcgct gacatcctct ggcgtcaatg
     2761 caacggtatt ttgataggtt ttttccgctt taccgtcatc gcctttaaag cggacattgg
     2821 aaaactcggt gccacccacc agacccggtt cgatgtcggt gacgcgcacc gccgtaccat
     2881 gcagatccgt acgcagattc aggctaaact gacgaacaaa cgctttcgtc gcaccgtaaa
     2941 cgttaccacc ggcatacggc cagctacctg ccgttgagcc aatgttaata atatgaccat
     3001 gattacgttc aaccataccc ggtaagacgg cgcgcgtcat atataccagg cctttattgt
     3061 tggtatcaat catcgtttcc cagtcttcaa cgctggcttt atgcgcaggc tccatgccca
     3121 acgccaggcc ggcattattt accaggatat caatattgct ccactcggca gggagcgatg
     3181 ccagcatctc ttcaatagcg gcacggttgc gaacgtccag ttgggcgata tacagattat
     3241 ctcccagttc gtcttttaac tcctgcaacc gttcctggcg acggccagtg gcgataactt
     3301 tatgcccttg ttgaataaaa cgacgagtaa tgcattcacc aaaacctgcc gttgctccag
     3361 ttactaaaac gatcatctca ctgttcctca acgcttttgt gtgtcaataa catagcacgg
     3421 gatgcttaag agcgggtaat actggtttgc aagattgcac tccgttatca gtaagcctac
     3481 gctatggaac acctcgcgtt caggagaaga aaatgacaac aatgaatcct ttccttgtgc
     3541 aaagcacact gccgtatctg gctccccatt ttgatcaaat tgccaatcat cactatcgcc
     3601 cggcattcga tgagggaatg ctgcaaaagc gggcagaaat tgctgccatc gcgcttaacc
     3661 cgcaaacacc tgatttcaac aatactattc tggcactgga acaaagcgga gaattactta
     3721 cccgcgttac cagcgtcttt tttgccatga ctgcggcgca taccaatgat gaattacagc
     3781 gtcttgacga gcagttttcc gctgaactgg cggaactggc taatgatatc tatctgaacg
     3841 gtgaattatt cgcgcgggta gatgctgtct ggcaacgccg tgaatctctg gggcttgata
     3901 gtgaatccat ccgcctggtg gaggtgattc atcaacgttt tgtcctagcc ggagccaaac
     3961 ttgcacaagc tgataaagca aaattaaaag tactgaatac agaagctgcg acactgacca
     4021 gtcagtttaa ccagcggtta ctggcagcaa ataaatccgg cagtctggtt gtgaacgata
     4081 tcgcgcagct ggcaggaatg agtgagcaag agattgcgct ggcggcagag gcggctcgcg
     4141 agaaaggtct ggataacaaa tggctgattc cgctgctgaa taccacccaa caaccggcgc
     4201 ttgccgaaat gcgcgatcgt gcgacgcgtg aaaaactgtt tattgcgggc tggacgcgag
     4261 cggaaaaaaa tgatgccaat gatacccgcg ctatcattca acgtctggtg gagatccgtg
     4321 cacaacaggc tacattactc ggttttcctc attatgccgc atggaaaatc gccgatcaga
     4381 tggcaaaaac acctgaagca gcacttaact ttatgcggga aattgttcca gcggcgcgtc
     4441 aacgtgcgag cgatgaatta gcctccatac aggcggttat cgataagcag cagggcgggt
     4501 ttagcgcgca gccgtgggac tggggatttt atgccgaaca ggtccggcgg gagaaatttg
     4561 atcttgatga ggcgcagctc aagccatatt ttgaattaaa cacggtgttg aatgaaggtg
     4621 tattctggac cgcgaatcag ctcttcggta ttaagtttgt cgaacgtttt gatattcctg
     4681 tctaccatcc tgacgttcgg gtgtgggaaa tttttgatca taatggcgtg gggctggcgt
     4741 tattttacgg tgatttcttc gcccgtgatt caaaaagcgg cggtgcatgg atgggcaatt
     4801 ttgttgagca atcaacgctt aatgaaacgc atccggtaat ttataacgtc tgcaattatc
     4861 agaaacccgc tgccggtgag cctgcgttgt tactctggga tgaagtcata accttattcc
     4921 atgaatttgg tcatacgcta cacggcctct ttgcccgcca gcgttatgcc acgctctccg
     4981 gtaccaacac gccgcgtgat tttgtcgaat ttccgtcgca aatcaacgaa cactgggcaa
     5041 cgcatccgca ggtattcgct cgctacgccc ggcattatca gagcggggca gcaatgcctg
     5101 acgaactgca acagaaaatg cgtaatgcca gcctgttcaa caaagggtat gagatgagcg
     5161 aactgcttag cgccgcactt ctcgatatgc gctggcattg cctggaagaa aacgaagcaa
     5221 tgcaggatgt cgatgatttc gaattgcggg cgctggtggc ggaaaatatg gatcttcctg
     5281 ctataccgcc acgctatcgc agcagttatt tcgcccatat ttttggtggc ggatatgccg
     5341 caggttatta cgcttatctg tggacgcaaa tgttggccga tgatggttat cagtggtttg
     5401 ttgagcaggg cggattaacg cgtgaaaatg ggctgcgttt tcgcgaggcg atcctttcca
     5461 gaggtaacag cgaagatctg gaacgcctgt atcgacaatg gcgcggtaag gctcctcaga
     5521 ttatgccgat gctgcaacat cgtggcttga acatataaac accaccaatg gctgtcctgg
     5581 agatatcgcc aggccagcct ggttatagca gtaattgcag cagctgggcg agggcaaatc
     5641 tcaccgctaa agctaatacc gtttcgcagt cgccagcaaa atgcataacc gcagtgtagt
     5701 tctggccttt aatatgccac gcgaaccaga cggtgcccgc tggcgtacca tcttcaccgc
     5761 cctccggtcc gccgtagccg gtaatggcaa tactgacatc agcatctgca cgctctatgg
     5821 caccggttgc catttctgct gccactttct cactcaccgc agaatatcgt tcaagagatt
     5881 gctggcttac gctgaggatt ttcatctttg cctgatcggt gaaagtaaca aagcctgcac
     5941 cgtaaaattt gggtgtatct tcagctgcac acagggcgct agccaactta ccgccggtgc
     6001 atgattcggc ggtagttaag cgtaattgat ccgcgataag tctttgactc aacgcggatg
     6061 tcaggttttc aatagtgtcg gtatctgcga gttgtacaat tttatcttta ttaatgttca
     6121 ctgttt
//
LOCUS       DAZKFU020000264         6061 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZKFU020000264 DAZKFU020000000
VERSION     DAZKFU020000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.266"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1218
                     /gene="astC"
                     /locus_tag="ACHOQQ_RS20760"
                     /old_locus_tag="pgaptmp_004152"
     CDS             <1..1218
                     /gene="astC"
                     /locus_tag="ACHOQQ_RS20760"
                     /old_locus_tag="pgaptmp_004152"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGU4472338.1"
                     /translation="SQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDFA
                     GGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFCN
                     SGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAPL
                     PPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNAL
                     LIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVGT
                     HGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFSEVRGL
                     GLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRF
                     AAACERFVSGGSS"
     gene            1215..2249
                     /gene="astA"
                     /locus_tag="ACHOQQ_RS20765"
                     /old_locus_tag="pgaptmp_004153"
     CDS             1215..2249
                     /gene="astA"
                     /locus_tag="ACHOQQ_RS20765"
                     /old_locus_tag="pgaptmp_004153"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGU4472339.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGIVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            2246..3724
                     /gene="astD"
                     /locus_tag="ACHOQQ_RS20770"
                     /old_locus_tag="pgaptmp_004154"
     CDS             2246..3724
                     /gene="astD"
                     /locus_tag="ACHOQQ_RS20770"
                     /old_locus_tag="pgaptmp_004154"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGU4472340.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAVFPRWARLSFAERQAVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPHLLQAGTSLLTPGIIEMTCVAGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFEQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            3721..5064
                     /gene="astB"
                     /locus_tag="ACHOQQ_RS20775"
                     /old_locus_tag="pgaptmp_004155"
     CDS             3721..5064
                     /gene="astB"
                     /locus_tag="ACHOQQ_RS20775"
                     /old_locus_tag="pgaptmp_004155"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGU4472341.1"
                     /translation="MNAWEVNFDGLVGLTHNYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREKGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWCY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            5057..6025
                     /gene="astE"
                     /locus_tag="ACHOQQ_RS20780"
                     /old_locus_tag="pgaptmp_004156"
     CDS             5057..6025
                     /gene="astE"
                     /locus_tag="ACHOQQ_RS20780"
                     /old_locus_tag="pgaptmp_004156"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGU4472342.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         1290 a         1545 c         1837 g         1389 t
ORIGIN      
        1 tctcagccaa ttacgcgtga aaactttgat gaatggatga tacctgttta cgctccggca
       61 ccgtttatac cggtacgtgg cgaaggttcg cgcttgtggg atcagcaggg gaaagagtat
      121 atcgacttcg cgggtggcat tgcggtgaac gcgctgggac atgcgcaccc ggaactgcgc
      181 gaggcgctga acgaacaggc gagtaagttc tggcataccg gcaacggtta caccaacgaa
      241 ccggtactgc gactggcgaa aaaattgatc gacgccacgt ttgccgatcg cgtcttcttt
      301 tgtaactctg gcgcggaagc caacgaagcg gcgctaaaac tggcgcgtaa attcgctcac
      361 gaccgctacg gcagccataa gagcggtatc gtggcgttta aaaatgcgtt tcatggtcgc
      421 acgctgttta ctgtcagtgc gggtgggcag ccagcctatt cacaggattt tgcgccactg
      481 ccaccagata tccgtcatgc tgcatataac gatattaact ctgccagcgc gctgattgac
      541 gactctacct gtgcggtgat tgtcgaaccc atccagggcg aaggcggtgt ggtgccagcc
      601 agcaacgcat ttttacaagg tctgcgtgaa ttgtgtgatc gccacaatgc gctgttgatt
      661 tttgatgaag tacaaaccgg cgtcgggcgc accggggaac tgtatgccta tatgcactac
      721 ggcgtgacgc ctgatctgtt aactaccgcc aaagcgctgg gcggcggttt cccggtcggt
      781 gcgttgttag caaccgaaga gtgcgccagc gtgatgaccg ttggcactca tggcaccacc
      841 tatggcggca atccgctggc ctcggcggtg gcaggcaaag tgctggagct catcaatacg
      901 ccagagatgc ttaacggggt taagcaacgt cacgactggt ttgttgagcg tcttaatatt
      961 attaatcacc gctatggttt gttcagtgaa gttcgcggct taggtttgct gattggctgt
     1021 gtactgaatg ccgattacgc cggacaagcg aaacagatct ctcaggaggc ggcgaaagcg
     1081 ggcgtgatgg tgctgattgc gggtggcaac gtggtgcgtt ttgcgcctgc gctcaatgtc
     1141 agcgaagaag aggtgacgac cggactggat cgctttgctg cggcctgtga acgctttgtg
     1201 agcggaggtt catcatgatg gtcatccgtc ccgttgaacg ctcagatgtc tcggcgctga
     1261 tgcagcttgc cagcaaaacg ggcggcggcc tgacgtcgct tcccgccaat gaagccacgc
     1321 tttcggcgcg tatcgaaagg gcaatcaaaa cctggcaagg tgaactgccc aaaagtgagc
     1381 agggttatgt gtttgtactg gaagatagcg agacaggcat tgtggcgggg atttgcgcca
     1441 ttgaggtggc agttgggctg aacgatccct ggtacaacta tcgcgtcggc acgttggttc
     1501 acgcttcgaa agagctgaat gtctataacg cgctgccaac gctgtttctc agtaacgatc
     1561 acaccggcag cagcgagctg tgcacgctgt ttctcgaccc ggactggcgc aaagagggca
     1621 acggctattt gctgtcgaaa tcgcgcttta tgtttatggc ggcttttcgc gacaagttta
     1681 atgacaaagt ggttgctgaa atgcgcgggg tgattgacga acacggctat tcaccgttct
     1741 ggcaaagcct cggtaaacgc ttcttttcga tggattttag ccgcgccgat tttctctgcg
     1801 gcaccgggca aaaggcattt attgcagaac tgatgccgaa acatccgatc tatacgcact
     1861 ttttatccca ggaggcccag gacgtcatcg gtcaggtaca tccgcaaacc gcgcctgccc
     1921 gcgcggtgct ggagaaagaa ggttttcgct accgtaatta tatcgacatc ttcgacggtg
     1981 ggccgacgct tgagtgtgac atcgaccgcg tgcgcgccat ccgtaaaagt cggctggtgg
     2041 aagtggcaga agggcagcct gcgcagggcg attttccggc ctgcctggtc gccaatgaaa
     2101 attatcacca tttccgcgtg gtgctggtgc gtaccgatcc ggcaaccgag cgtttgattt
     2161 taaccgccgc acaactggat gccctcaaat gccacgcagg ggatcgcgtt cgtctggtgc
     2221 gcctgtgcgc agaggagaaa acagcatgac tttatggatt aacggtgact ggataacggg
     2281 ccagggtgca tcgcgtgtga agcgtaatcc ggtatcgggc gaggtgttat ggcaaggcaa
     2341 tgatgccgat gccgctcagg tcgggcaggc ttgtcgcgca gcccgtgcgg tgtttccgcg
     2401 ctgggcgcgg ctctcatttg ctgaacgtca ggcagttgtc gaacgctttg ccggattgct
     2461 ggaaagcaat aaagccgaat taaccgcaat cattgcccga gaaaccggca agccgcgctg
     2521 ggaagcggca accgaagtga cggcgatgat caataaaatc gcgatttcaa ttaaggcgta
     2581 tcacgttcgt accggcgagc agcgtagtga aatgccggac ggcgcggcga gcctgcgaca
     2641 tcgcccgcac ggcgtgctgg cggtgtttgg gccgtataat ttccctggtc atttgccgaa
     2701 cggacatatc gttccggcat tgctggcagg taacaccatt atctttaaac ccagcgaact
     2761 gacaccgtgg agtggcgaag cggtaatgcg tttatggcag caggctggct tgccgccggg
     2821 cgtgctgaac ctggtgcagg gcgggcgtga aacgggtcag gcgctgagtg cgctggagga
     2881 tctcgacggc ttgctgttta ccggtagcgc caataccggc taccagctgc atcgccagct
     2941 ctccggtcag ccggagaaaa ttctcgccct tgagatgggc ggtaataatc cgctaattat
     3001 cgatgaggtg gcggatatcg acgccgctgt ccatctgacc attcagtcgg cgtttgtcac
     3061 agccggtcaa cgctgcacct gcgcccgccg tttattgctg aaaagcgggg cgcagggcga
     3121 tgcgtttctt gctcgtctgg ttgccgtcag ccagcgatta acgccgggca actgggatga
     3181 cgaaccgcag ccgtttattg gcgggctgat ttctgaacag gccgcacagc aggtggttac
     3241 tgcctggcag caactggaag cgatgggcgg acgaaccctg cttgcgccgc acttgctgca
     3301 agccggaaca tcgttgctga cgccggggat cattgaaatg acgtgcgttg ctggcgtgcc
     3361 ggatgaagaa gtgtttgggc cactcttgcg cgtctggcgt tatgacaatt tcgatgaggc
     3421 gatcaggatg gcgaataaca ctcgcttcgg cctctcttgc ggtctggttt cccccgagcg
     3481 ggaaaagttc gaacaactgt tgctggaagc gcgggcgggg attgttaact ggaacaaacc
     3541 gcttactggt gctgccagta ccgcgccatt cggcggcatt ggtgcatccg gtaaccatcg
     3601 ccccagcgcc tggtatgccg cagattactg cgcatggcct atggcgagcc tggagtcgga
     3661 ctcgttaacg ttacccgcca cgcttaaccc cggactggat ttttccgatg aggtggtgcg
     3721 atgaacgctt gggaagttaa tttcgacggg ctggtagggc tgacacacaa ttacgctggc
     3781 ctgtcgtttg gtaatgaagc ctctacccgt caccgttttc aggtgtctaa cccgcgactg
     3841 gcggcgaaac agggcttact gaaaatgaaa acccttgccg atgcgggatt cccccaggcc
     3901 gtgatcccgc cgcacgagcg tccgtttatt ccggtgctgc gtcagttggg attcagtggt
     3961 agcgatgagc aggtactgga aaaagttgca cgccaggcac cgcactggct ttccagcgtc
     4021 agctccgctt cgccaatgtg ggtagccaat gcggcaacga tcgcgccatc tgccgatacg
     4081 ctggatggca aagtgcatct caccgttgcc aacctgaaca ataaatttca ccgttcgctg
     4141 gaagcgcccg tcactgaatc gctgttaaaa gctattttta acgacgaaga gaaatttagc
     4201 gtccattcgg cgttgccgca ggtagcgttg ctcggtgatg agggggcggc aaaccacaat
     4261 cgtctcggcg gtcattacgg tgaaccgggt atgcaacttt ttgtctacgg gcgagaaaaa
     4321 ggcaatgata cccggccttc ccgttatccg gcgcgacaga ctcgcgaagc cagcgaggct
     4381 gtggcaaggc tgaatcaggt gaatccccaa caggtgattt tcgcccagca aaacccggac
     4441 gttatcgacc agggcgtttt tcataatgac gtgattgccg tgagtaaccg ccaggtgctg
     4501 ttttgccacc aacaggcgtt cgctcgccag tcacagttac tggcaaacct gcgtgcgcgg
     4561 gtgaacggtt ttatggcgat agaagttccg gcaactcagg tttccgtgtc ggatgcggtg
     4621 tctacatatc tgtttaacag ccaactgttg agccgcgatg atggttccat gatgttggtg
     4681 ctgccacagg agtgtcggga acacgccgga gtatggtgtt atctcaatga actccttgcc
     4741 gctgacaacc cgatcagcga actaaaagtc tttgatttac gtgaaagcat ggcgaatggc
     4801 ggtggcccgg cgtgcctgcg gttgcgggtg gtattgacac aagaagaacg ccgggcggtg
     4861 aatccggcgg tgatgatgaa cgataccttg tttaatgcgc tcaatgactg ggtggatcgt
     4921 tactaccgcg atcgtcttac tgctgccgat ctggccgacc cgcaattgct gcgcgaaggg
     4981 cgggaagcac tggatgtatt gagccaatta ctgaatctcg gttcggttta tccgttccag
     5041 cgcgagggag ggggcaatgg ataattttct tgctctgacc ttaacgagta aaaaaccggt
     5101 tatcaccgag cgagaaatca acggtgttcg ctggcgctgg ctgggcgatg gtgtgctgga
     5161 actgacgcca ttaacgccac cgcaaggcgc actggtgatt tcagcgggga tccacggtaa
     5221 tgaaacggct ccggtggaaa tgctggacgc gttgcttggc gcgatatctc acggcgagat
     5281 cccgttacgt tggcggttgc tggtgatcct cgggaatcct cctgcgctga agcaagggaa
     5341 acgttattgc catagcgata tgaaccgaat gtttggcggt cgttggcagc tgtttgctga
     5401 aagcggagaa acctgtcggg cgcgcgaact ggaacagtgc ctggaagatt tttatgacca
     5461 gggcaaagaa tctgtgcgct ggcaccttga tctacatacc gcaattcgtg gctccttgca
     5521 tccgcagttc ggtgtattac cgcaacgcga cattccctgg gacgagaaat ttctgacgtg
     5581 gctgggtgcg gcggggctgg aggcgctggt gttccatcag gagcctggtg gtacgtttac
     5641 ccatttcagc gccagacatt ttggcgcgct ggcctgtacg ctggaacttg gcaaagcgtt
     5701 gccctttggg caaaacgatc ttcgccagtt tgcagtaact gccagcgcaa ttgctgcgct
     5761 gctatctggt gagagtgtcg gtatcgtgag aacaccgccg ctccgttatc gggtggtttc
     5821 gcaaattact cgccactcgc cgtccttcga aatgcatatg gcaagtgaca cgctgaattt
     5881 tatgccgttt aagaaaggaa cgttgctggc gcaggacggt gaggaacgtt ttaccgtaac
     5941 ccatgatgta gagtatgtgt tattccctaa tccgttggta gcgttggggt tacgcgcggg
     6001 attaatgctc gaaaaaataa gctaatactt acccgcagaa atcattctgc gggtattaag
     6061 t
//
LOCUS       DAZKFU020000265         6046 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZKFU020000265 DAZKFU020000000
VERSION     DAZKFU020000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.267"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(50..1441)
                     /gene="tcyP"
                     /locus_tag="ACHOQQ_RS20785"
                     /old_locus_tag="pgaptmp_004157"
     CDS             complement(50..1441)
                     /gene="tcyP"
                     /locus_tag="ACHOQQ_RS20785"
                     /old_locus_tag="pgaptmp_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGU4472343.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQILKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1574..2164)
                     /gene="ydjM"
                     /locus_tag="ACHOQQ_RS20790"
                     /old_locus_tag="pgaptmp_004158"
     CDS             complement(1574..2164)
                     /gene="ydjM"
                     /locus_tag="ACHOQQ_RS20790"
                     /old_locus_tag="pgaptmp_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4472344.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2327..2995)
                     /gene="hxpB"
                     /locus_tag="ACHOQQ_RS20795"
                     /old_locus_tag="pgaptmp_004159"
     CDS             complement(2327..2995)
                     /gene="hxpB"
                     /locus_tag="ACHOQQ_RS20795"
                     /old_locus_tag="pgaptmp_004159"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGU4472345.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3142..3678
                     /gene="yniB"
                     /locus_tag="ACHOQQ_RS20800"
                     /old_locus_tag="pgaptmp_004160"
     CDS             3142..3678
                     /gene="yniB"
                     /locus_tag="ACHOQQ_RS20800"
                     /old_locus_tag="pgaptmp_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGU4472346.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3719..4579)
                     /gene="yniA"
                     /locus_tag="ACHOQQ_RS20805"
                     /old_locus_tag="pgaptmp_004161"
     CDS             complement(3719..4579)
                     /gene="yniA"
                     /locus_tag="ACHOQQ_RS20805"
                     /old_locus_tag="pgaptmp_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGU4472347.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4685..4975)
                     /gene="ghoS"
                     /locus_tag="ACHOQQ_RS20810"
                     /old_locus_tag="pgaptmp_004162"
     CDS             complement(4685..4975)
                     /gene="ghoS"
                     /locus_tag="ACHOQQ_RS20810"
                     /old_locus_tag="pgaptmp_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4472348.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5076..6005)
                     /gene="pfkB"
                     /locus_tag="ACHOQQ_RS20815"
                     /old_locus_tag="pgaptmp_004163"
     CDS             complement(5076..6005)
                     /gene="pfkB"
                     /locus_tag="ACHOQQ_RS20815"
                     /old_locus_tag="pgaptmp_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGU4472349.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1540 a         1539 c         1499 g         1468 t
ORIGIN      
        1 aagtaaggca acggccccta tacaaaacgg accgttgcca gcataagaat taatggtgtg
       61 ccagttcggc gtcgtcttcg ctatccagaa tggctttatc ggtttgcttc agccactggc
      121 tggtcagcgt gccagctgtc atcgagccac taacgtttag cgccgtacgg cccatgtcga
      181 taagcggttc aacggagatt aacagcgcca ccagggttac tggcaggccc atcgcaggca
      241 gcacaatcag tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct gcggagctaa
      301 cggtaacaat accgaccagc gtcgcaatcc acatcgggtc cagcgggtta atgccaaccg
      361 taggcgcaac catcaccgcc agcattgccg gatacaaacc ggcgcagccg ttctgaccaa
      421 tggtcgcacc gaaagaggcg gcgaaactgg cgatggattc aggaacgccc agacgacgcg
      481 tttgtgcttc cacattcagt gggatagacg cagcactgga acggctggta aaggcaaacg
      541 tcagcacagg ccatacctta cggaagtact tcagcggact cacgccatta atgcccagca
      601 gaatgccatg cactgcaaac ataatcagca gaccgaggta ggacgcgaca acgaaacttc
      661 ccagtttgat gatgtcttgc aggttagaac ctgcaaccac tttggtcatt agtgccagaa
      721 cgccgtaagg ggtcaactgc atgaccaggc gaaccagttt catcacccag ctttgcaggg
      781 tatcgatagc ggttaagacg cgttcacctt tcggcgcatc atccttcagc agtttcagcg
      841 cagctacacc gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc ggattggctc
      901 cggtcagatc ggcaaacggg tttttcggga taaaggacaa gaccagctgc ggaacgctca
      961 ggtcagagac tttaccaaca tagttacttt cgatggcgtt cagacgtgca gtttctgcac
     1021 caccctgaac cagaccttca gccgtcaaac caaacaggtt ggtgaccagc acaccgacca
     1081 gcgccgcaat cagcgtggta aacaaaagcg taccgatggt cagaaaactg attttgccta
     1141 actgagatgc gttatgcaga cgggcaaccg cgctcagaat agaggcgaag actaacggca
     1201 taacgatcat ttgcagcagt tgaacatagc cgttaccaac gatgttaaac cactgtacag
     1261 aatctttaag tatctggctg tcagaaccat aaatggtatg cagggcaagg ccaaaaacca
     1321 cacccatcac cagacccacc agcacttttt tcgccagact ccactgttta tggcgggtct
     1381 gagccagcgc aaacagcagt acaacgaaca ccacgatgtt cgcaattaat ggaaagttca
     1441 tccccgttct cctgatctta ttatttctat cactgcgtag cagtcatagt gttggcgcaa
     1501 gattaacaga actgtgagct ggctcttata ttcaaatgga attgcttatt ccagaaaggt
     1561 gatttttgcc tttttagtat tcaacctgat gcttaataag ccgatgaaac tggatctgca
     1621 aggtattgat catttgcgat gaccaacgaa cagcgctgtt ctcgggtaat gaatggggca
     1681 tccataccga ccagacaaat aatgccatgc agataaaacg ttccagttgg ttaccctttt
     1741 ggggaaccag gataggcaag cggaaacgcc agcggcatgg ccagagcagg ggaacaccgg
     1801 cgggtgtcag catatcggca agtatgtggc ttaaataacc cagcaccatt ccttgtagcg
     1861 catcagccgg aatgaaccag ctttcaggaa ccttaaggta aaaggttgcc agcagcgcaa
     1921 ataccgccag cagactgtgg gtaaaaccac gatgcccaaa agcgcgggcg atcggttttg
     1981 atatccactt taatcgctgc ccaagaaacg actttgggtg atcgatgtcc ggtaacaaac
     2041 acgtcaggat tgcggaaggg acaatatgcc accagtcacc ctgtgccagc acgggcgtca
     2101 gctcggcatt tttggcaaat accgcacaag caatagaaaa gagaaggtga ccttccgccg
     2161 tcatgataaa actcactaaa ctgtcgatac gtacagtata ggatttttat acagtgcacg
     2221 gaagtggtga cggtgtaact ctttctctta aattgacgat taatccgtca gttgaatgca
     2281 aattcagata aagaaccagg ggcagcgtga tcactgcccc tggtcattaa ccgagaaggt
     2341 cttttgcggt gagttctgtc agcgatgaga gtttgacgtt tgctaataca aaacgtggat
     2401 cattttgcgc ttctggcgca ggaacgacga tggaacgcat gcgggctgct ttagaggcga
     2461 tcatgccatt taccgaatcc tctagcgcta cgcaggtcag tgggtcaacg cccagttttg
     2521 ctgcgcagtc gagatatact tgcggatgcg gcttgctgta aggcagtttt tcggccgagg
     2581 cgagggcatc gaaactgtcg cgtaagtcaa acatggtcaa cactttttcc agcatatgta
     2641 gtggtgacgc ggaggccagt cccaccaata aaccttgttc tttgcataac gcaacggctt
     2701 cgcgcacgcc tggtaataat ggacgtgtct cttcaaccag tgaaatggca cgggcaataa
     2761 cccgttctac tacttcctga cggcttggcc cattccacgg ttgccgggcg taccaaagat
     2821 cgaccaccat atcgatgcgt aaacctaagg tgtccggcag ctcgttacga cgggagatat
     2881 ccacccccag gcttgccatc acatccagtt cggctcgatc ccataaaggt tctgagtcga
     2941 taagtaatcc atccatatca aaaattgcag caagaatctg acgcggggtt gacattacaa
     3001 cctctccttt agagcattat gtgtaaggta ttaagctgtg cttatgatta agcgcctatc
     3061 atatcgcctt taaagaatgg gcgaaaacag atatctacag gtaaactaag tgaaacgacg
     3121 cgtcttaatc aaggagaact catgacgtat caacaagctg gacgcattgc tgttttgaaa
     3181 aggattatgg gttgggtgat ttttatacct gcattgatct ctacattgat ttctttgctg
     3241 aaatttatga atacccgaca ggaaaaccag gaaggcatta atgcggtcat gctcgacttt
     3301 actcatgtca tgatcgatat gatgcaggcg aatacgcctt ttttgaatct attctggtat
     3361 aactccccga cgcccaattt taatggtggc gtgaacgtta tgttctgggt gattttcatc
     3421 ctgatttttg tcggactggc gttgcaggat tccggtgccc ggatgagtcg ccaggcgcgc
     3481 ttcttgcggg aaggtgttga agatcaactc attctggaaa aagccaaagg ggaagagggg
     3541 ctaacgcgtg aacaaattga gtcccgtatt gttgttccgc accatactat tttcctgcag
     3601 tttttctcac tgtatatcct gccggttatt tgtattgctg caggttatgt gttcttttct
     3661 ctgcttgggt ttatttaatc aaaaggccgc acggagcggc cattatcctc aacccatatc
     3721 atgctgctaa taatctatcc aatgactgct gagcaataac caaatgctga ccgccaaata
     3781 accttgctcg atttaacagc gtgtagagtt ggtaaaccgg ttgacgttca aggaaatcgg
     3841 caggtagcgg ggatactgac tgatagccgt cataaatttg tggcggctgt tcagtatgca
     3901 gcggtaacat cgccaggtcg cactctcggt caccccagta gcatgccggg tcgaaaatgt
     3961 acgggccatc cggacccagc gcacagttgc cggaccataa atcgccgtgc aacagagaag
     4021 gctgcggttg atgtgaggcg agacgctgct gaatatgctc gacgatggcg tcgatattgc
     4081 cgaaagcgat ccctttttct gctgccagtt ccaactgcca gccaatccgt tgttcagcaa
     4141 aaaacgttga ccagcgacgt tgccaggtgt tgggctgtgg agttgtggag agcgcgttat
     4201 cgaaatcgag gccaaattgt ggttggtcac tccattgatg taaacgcgca atttgctgac
     4261 caagaataaa tgcgctatgc gcatccagcg gacgaggtgg gagataatcc atcaccagaa
     4321 aactgtagtc acggtcagcg cctactgccc aaaccttagg cacggtgacg gttttactac
     4381 gcgacagtaa ctccagttgg tcggcttcgg cggtaaaacc gggaagcagt tccctttcat
     4441 cacatttgac gaaaaagtca tgtcctgcat agcgcaaatg ccatgcggca tggacttctc
     4501 cgccaggcag ttcattacgc agttcgattt cgccttcacc taactgctcg ctcaaaagac
     4561 gactgattgc ctgccacatg atgtctctcc catgttgtct gccagatcat aaggttagcg
     4621 caagaatgcg gtgtaaaaac acgagctaac gcacaccaca gactgattat tgcccaaaaa
     4681 ccatttattt tctgttgcta ttccattcct cccaggtcgt gatggtgatt tcaggatctt
     4741 tgccggttgc actttgggta agccccgaaa ccagttctct aatttcttct tcactttggg
     4801 tactgataag tccaaaagtg ttagtcccca gctcatggat atttccctca tcatccgtca
     4861 tggtgagcaa aaaaccgtcg cgagtcaggt aattattcaa ctcgttaatt tcagtcaatg
     4921 tatcctcatg caacatgacg gttatgacat aacggacaag atcgccactg gccatagttc
     4981 acccctttgt tatcacatga cttttgagca tagtcggaga aacgcgttgc cgacaggttg
     5041 gtgatgattc ccccaatgct gggggaatgt ttttgttagc gggaaaggta agcgtaaatt
     5101 ttttgcgtat cgtcatggga gcacagacgt gttccctgat tgagtgtggc tgcactcccc
     5161 gcagctacgc caaaacgaac catctcttca agagaggcat tttctgccag tttcagtgtc
     5221 atcgcgccga ccatgctgtc accagcgcca acggtactct ggcttttcac cggtggtggc
     5281 accacctgaa tgcagttttc actatcaaca cccagcgctc cttgtggacc cagggaaacg
     5341 acaacccgtt tggccttgcc gctattaacg atttcttgcg cggctttgcg gacatcgtct
     5401 ggctgggtga gttcgcgatt caccagcgca ctgagttctt tttggttagg cttaaccaac
     5461 tcgatgttac caatcgccag tgctgcactt aacgcttcgc cagaactgtc gatgatgcag
     5521 cggatccctt ggttttgcgc agcggaaatc agttgggtta atttttccag cttcacacct
     5581 ggcggcaggc ttccgcttat gaccaggatg gccccggact caatttccag aacttgctct
     5641 tcaagctggc gaaactcatc ttcatttaat gccgcgcctg gcataacaaa acgatactgc
     5701 tcaccgcttg cttccacatg tacgtgtaaa ttctgccggg tccagtcttt ggcttctaca
     5761 gtagcgacgg ggacattttc atccgccaac agtgaaacca ggtgttcgcc ggtcgcgcca
     5821 cccgccggga agatcgctgt ggcactgcct ccaagatggg caatggcgcg ggcgacgttg
     5881 atgccgccgc ccccgggttc gaacaccggt gcggtacagc gcagttttcc ttcgggataa
     5941 atttgcgggg taattgttgc gctatcgaga gagggcgcaa gtgtcaacgt atagatacgt
     6001 accatcattt cctcctatag gctgatttca gtctggcacc gaatca
//
LOCUS       DAZKFU020000266         6013 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZKFU020000266 DAZKFU020000000
VERSION     DAZKFU020000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.268"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            155..1282
                     /gene="ompC"
                     /locus_tag="ACHOQQ_RS20820"
                     /old_locus_tag="pgaptmp_004164"
     CDS             155..1282
                     /gene="ompC"
                     /locus_tag="ACHOQQ_RS20820"
                     /old_locus_tag="pgaptmp_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4472350.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNAPESENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGYATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLG
                     WANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1394..2449
                     /gene="apbE"
                     /locus_tag="ACHOQQ_RS20825"
                     /old_locus_tag="pgaptmp_004165"
     CDS             1394..2449
                     /gene="apbE"
                     /locus_tag="ACHOQQ_RS20825"
                     /old_locus_tag="pgaptmp_004165"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGU4472351.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2523..3587
                     /gene="ada"
                     /locus_tag="ACHOQQ_RS20830"
                     /old_locus_tag="pgaptmp_004166"
     CDS             2523..3587
                     /gene="ada"
                     /locus_tag="ACHOQQ_RS20830"
                     /old_locus_tag="pgaptmp_004166"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGU4472352.1"
                     /translation="MKNATRLTEDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYADASEALAAGFRPCKRCQPDKANAQQHRLDKITRACRLLEQEMPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRYGGENLAVRYALADCEQGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3587..4237
                     /gene="alkB"
                     /locus_tag="ACHOQQ_RS20835"
                     /old_locus_tag="pgaptmp_004167"
     CDS             3587..4237
                     /gene="alkB"
                     /locus_tag="ACHOQQ_RS20835"
                     /old_locus_tag="pgaptmp_004167"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGU4472353.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4313..5956
                     /gene="yojI"
                     /locus_tag="ACHOQQ_RS20840"
                     /old_locus_tag="pgaptmp_004168"
     CDS             4313..5956
                     /gene="yojI"
                     /locus_tag="ACHOQQ_RS20840"
                     /old_locus_tag="pgaptmp_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGU4472354.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1417 a         1507 c         1649 g         1440 t
ORIGIN      
        1 ataaatgaaa catcttaaaa gttttagtat catattcgtg ttggattatt ctgcattttt
       61 ggggagaatg gacttgccga ctgattaatg agggttaatc agtatgcagt ggcataaaaa
      121 agcaaataaa ggcatataac agagggttaa taacatgaaa gttaaagtac tgtccctcct
      181 ggtcccagct ctgctggtag caggcgcagc aaacgctgct gaagtttaca acaaagacgg
      241 caacaaatta gatctgtacg gtaaagtaga cggcctgcac tatttctctg acgacaagtc
      301 tgtagatggc gaccagacct acatgcgtct tggcttcaaa ggtgaaactc aggttactga
      361 ccagctgacc ggttacggtc agtgggaata tcagatccag ggcaacgctc cggaaagcga
      421 aaacaactcc tggacccgtg tggcatttgc tggtctgaaa ttccaggatg tgggttcttt
      481 cgactacggt cgtaactacg gcgttgttta tgacgtaact tcctggaccg acgtactgcc
      541 agaatttggt ggtgacactt acggttctga caacttcatg cagcagcgtg gtaatggcta
      601 cgcaacctac cgtaacaccg acttcttcgg tctggttgac ggtctgaact ttgctgttca
      661 gtaccagggc aaaaatggta gcgtaagcgg cgaaggcatg accaacaatg gtcgtggtgc
      721 tctgcgtcag aatggcgacg gtgtcggcgg atctatcact tatgattacg aaggcttcgg
      781 tatcggtgct gcagtttcca gctccaaacg tactgatgat caaaatggta gctacatcag
      841 caatggtgta gttcgtaact acatcggtac tggcgaccgt gctgaaacct acaccggtgg
      901 tctgaaatac gatgctaaca acatctacct ggctgctcag tacacccaga cctacaacgc
      961 aactcgcgta ggcaacttgg gttgggcgaa caaagcacag aacttcgaag ctgttgctca
     1021 gtaccagttc gacttcggtc tgcgtccgtc cctggcttac ctgcagtcta aaggtaaaaa
     1081 cctgggtgtc atcaatggtc gtaactacga cgacgaagat atcctgaaat atgttgatgt
     1141 tggtgcgacc tactacttca acaaaaacat gtccacctac gttgactaca aaatcaacct
     1201 gctggacgac aaccagttca ctcgtgacgc tggcatcaac actgataaca tcgtagctct
     1261 gggtctggtt taccagttct aatctcgatt gatatcgaac aaagggcctg cgggcccttt
     1321 tttcattgtt ttcagcgtac aaactcagtt ttttggtgta ctcttgcgac cgttcgcatg
     1381 aggataatca cgtatggaaa taagctttac ccgcgtggca ctgctggctg ccgcgctctt
     1441 ctttgttggt tgcgatcaaa aaccacaacc cgccaaaacc cacgctactg aagttaccgt
     1501 tcttgaaggc aaaaccatgg gtaccttctg gcgtgccagc atcccgggca ttgatgccaa
     1561 acgcagtgcc gaacttaaag aaaagattca gacccagctg gacgccgacg atcagctgct
     1621 ttcgacctat aaaaaagatt ccgcgctgat gcgctttaac gactcgcaaa gtttgtcgcc
     1681 gtggccagta agtgaagcga tggccgaaat cgtcactacc tcgctgcgca ttggcgcgaa
     1741 gaccgatggt gcgatggata ttaccgtcgg gccgctggtg aatctgtggg gctttggccc
     1801 ggaacaacag ccggttcaaa ttccgagcca ggaacagatc gatgcgatga aagccaaaac
     1861 cggcttacag cacctgacgg tcattaatca gtcgcatcag caatatctgc aaaaagacct
     1921 gccggattta tatgtcgatc tctctaccgt cggcgaaggt tatgcggcgg atcacctggc
     1981 acgcttgatg gagcaggaag ggatttcccg ctatctggtg tcggtgggcg gcgcgctgaa
     2041 cagccgtggt atgaacggtg aaggccagcc gtggcgggta gcgattcaaa agccaaccga
     2101 taaagaaaat gcggttcagg ccgtggtgga tatcaacggt catggtatta gcacctctgg
     2161 cagctaccgt aactattacg aactggacgg caaacgtctt tcccatgtta tcgatccgca
     2221 aaccgggcgt cccatcgaac acaatctggt atccgtgacg gtgattgctc cgacggcgct
     2281 ggaagccgat gcctgggata ctggcttgat ggtactcggg ccggagaaag ccaaagaggt
     2341 tgttcgccgg gaagggctgg cggtttatat gatcaccaaa gagggcgata gctttaaaac
     2401 ctggatgtcg ccgcagttta aaagcttcct ggtgagcgaa aaaaattaaa gcgcaagatt
     2461 gttggttttt gcgtgatggt gaccgggcag actaaaggct atccttaacc agggagctga
     2521 ttatgaaaaa cgccacacgc ttaactgaag atcaacgctg gcaatctgtc ttagcccgcg
     2581 acccgaatgc cgacggcgaa ttcgttttcg ccgtgcgcac cacaggtatc ttttgccgtc
     2641 cgtcttgccg cgccagacat gccttgcggg aaaacgtttc tttctatgct gatgccagcg
     2701 aggcgcttgc cgctggcttt cgtccctgca aacgctgcca acctgataaa gccaacgccc
     2761 agcaacatcg gctggataaa atcacccgcg cttgtcgact gctggaacag gaaatgcctg
     2821 taacgctgga ggccttagct gaccaggtgg cgatgagtcc gttccatctg catcgtttat
     2881 ttaaagccac gaccggaatg acgccaaaag cctggcaaca ggcctggcgc gctcgccgtt
     2941 tgcgcgaatc gctggcgaaa ggggagagcg tgacgacgtc tattcttaac gccggattcc
     3001 ccgacagcag cagttactac cgcaaagccg atgaaacgct gggcatgacg gctaaacaat
     3061 tccgttacgg tggcgaaaat ctggcggtgc gttacgcgct ggctgattgt gagcagggtc
     3121 gttgcctggt ggcagaaagc gagcggggga tttgcgcgat attgctgggc gatgatgacg
     3181 cgacactaat cagcgagttg cagcagatgt ttcccgctgc cgacaacgcg cctgccgatc
     3241 tgacgtttca gcaacatgtg cgtgaagtga tcgccagcct caatcaacgc gatacgccgc
     3301 tgacgttacc gctggatatt cgcggcactg cttttcagca acaagtctgg caggcactgc
     3361 gcacgatacc ttgcggtgaa accgtcagtt atcagcaact ggctaacgcc atcggcaaac
     3421 cgaaagcggt acgggccgtt gccagcgcct gtgccgccaa caagctggct atcgtaatac
     3481 cctgtcatcg agtggtccgt ggtgatggca cactttccgg ttaccgctgg ggcgtgtcgc
     3541 gtaaagcgca actgctgcgc cgcgaagctg aaaatgagga gaggtaatgt tggatctgtt
     3601 tgccgatgct gaaccgtggc aagagccact ggcggctggt gcggtaattt tacggcgttt
     3661 tgcttttaac gctgcggagc aactaatccg cgatattaat gacgttgcca gccagtcgcc
     3721 gtttcgccag atggtcactc caggcggata taccatgtcg gtggcgatga ccaactgtgg
     3781 gcatctgggc tggacgagcc atcggcaagg ttatctctat tcgcccattg atccgcaaac
     3841 aaataaaccg tggcccgcca tgccgcagag ttttcataat ttatgtcaac gtgcggctac
     3901 ggcggcgggc tatccagatt tccagccaga tgcttgtctt atcaaccgct acgctcctgg
     3961 cgcgaaactg tcgctgcatc aggataaaga cgaaccggat ctgcgcgcgc caattgtttc
     4021 tgtttctctg ggcttacccg cgatttttca atttggtggc ctgaaacgta atgatccgct
     4081 caaacgtttg ttgttggaac atggcgatgt ggtggtatgg ggcggtgaat cgcggctgtt
     4141 ttatcacggt attcaaccgt tgaaagcggg gtttcatcca ctcaccaccg actgccgcta
     4201 caacctgaca ttccgtcagg caggtaaaaa agaataaaaa taagaattat tattgctgtg
     4261 cgcgcgaaga tgtttaaact gcgggctgta attcattgtc cgggttttct gcatggaact
     4321 tcttgtactt gtctggcggc agtatcgctg gccatttatc agtgtgatgg cgctaagcct
     4381 cgccagtgcg gcattaggca ttggcttaat tgcttttatc aaccagcgcc ttatcgaaac
     4441 ggcggatacc agtctgctgg tgttgccgga gtttctggga ttattgctgc tgttgatggc
     4501 agtcactctc ggatcgcaac tggcgctcac cactttgggg catcacttcg tttaccgact
     4561 gcgtagtgaa tttatcaagc ggattctgga tacccacgtc gagcgcattg aacaactcgg
     4621 tagcgcctcg ttgctggcgg gattaaccag cgatgtgcgc aatatcacca ttgcttttgt
     4681 gcgtctgccg gaactggtgc aggggatcat tctcactatc ggttcagcgg cgtatctgtg
     4741 gatgctgtcg ggcaaaatgt tgctggtaac ggctatctgg atggcagtca ccatctgggg
     4801 cggttttgtg ctggtggcgc gggtgtacaa acatatggcg accctgcgtg aaaccgaaga
     4861 caagctgtac acggattttc aaactgtact tgaagggcgc aaagagctga ctctgaaccg
     4921 ggaacgcgcc gagtatgtgt ttaacaacct ctacattcct gatgcgcaag agtatcgcca
     4981 tcatattatt cgcgcagaca ccttccatct tagtgccgtg aactggtcaa acatcatgat
     5041 gctgggcgca atcggcctgg tgttctggat ggcgaacagc ctcggttggg ctgataccaa
     5101 cgttgccgcg acctattcgt tgacgctttt attcctgcgt acgccgctgc tttcggcggt
     5161 tggcgcattg ccgacgctgc tgacggcgca ggtggcgttt aacaagctga acaaattcgc
     5221 gctcgcgcct ttcaaagcag agtttccgcg cccgcaggcg tttcccaact ggcaaacgct
     5281 ggagctgcgt aacgtgacgt ttgcttatca ggataacgcg ttttccgttg gtccgattaa
     5341 tctcaccatc aaacgtggcg agctgctgtt tctgattggc ggcaacggta gcggaaaatc
     5401 gacgctggcg atgttgttga cgggcttgta tcagccacaa agcggcgaaa tcttgctgga
     5461 tggcaaacct gtcagcggcg aacaaccgga agattatcgc aaactgtttt cggcagtgtt
     5521 taccgatgtc tggctgtttg atcaactgct ggggccggag ggtaaacccg ctaacccgca
     5581 actggttgag aagtggctgg cgcagctgaa aatggctcat aagcttgagt taagcaacgg
     5641 gcgtattgtt aacctgaagt tatcaaaagg gcagaaaaaa cgcgtggcgc tgttgctggc
     5701 gctggcagaa gaacgcgata ttatcctgct ggatgaatgg gcggcggatc aggatccaca
     5761 cttccgtcgt gagttttatc aggtgttgct gccgctgatg caggagatgg gtaaaactat
     5821 tttcgccatc agtcatgatg atcattactt tatccacgcc gaccgcctgc tggagatgcg
     5881 caacgggcaa cttagcgagc tgacgggcga agagcgcgat gccgcttcgc gtgatgccgt
     5941 tgcccggacg gcataatatt acgacgctga cctggcggac tgctgccgcc aggtcaatat
     6001 gctaaaaatc cct
//
LOCUS       DAZKFU020000267         5999 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZKFU020000267 DAZKFU020000000
VERSION     DAZKFU020000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.269"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(177..1142)
                     /gene="alx"
                     /locus_tag="ACHOQQ_RS20845"
                     /old_locus_tag="pgaptmp_004169"
     CDS             complement(177..1142)
                     /gene="alx"
                     /locus_tag="ACHOQQ_RS20845"
                     /old_locus_tag="pgaptmp_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGU4472355.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(1426..2412)
                     /locus_tag="ACHOQQ_RS20850"
                     /old_locus_tag="pgaptmp_004170"
     CDS             complement(1426..2412)
                     /locus_tag="ACHOQQ_RS20850"
                     /old_locus_tag="pgaptmp_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4472356.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKQ"
     gene            complement(2491..3183)
                     /locus_tag="ACHOQQ_RS20855"
                     /old_locus_tag="pgaptmp_004171"
     CDS             complement(2491..3183)
                     /locus_tag="ACHOQQ_RS20855"
                     /old_locus_tag="pgaptmp_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HGU4472357.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATLFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARISAVIDAKIFHRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(3260..3763)
                     /locus_tag="ACHOQQ_RS20860"
                     /old_locus_tag="pgaptmp_004172"
     CDS             complement(3260..3763)
                     /locus_tag="ACHOQQ_RS20860"
                     /old_locus_tag="pgaptmp_004172"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGU4472358.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            3848..4984
                     /gene="rlmG"
                     /locus_tag="ACHOQQ_RS20865"
                     /old_locus_tag="pgaptmp_004173"
     CDS             3848..4984
                     /gene="rlmG"
                     /locus_tag="ACHOQQ_RS20865"
                     /old_locus_tag="pgaptmp_004173"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGU4472359.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAREMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            5268..5582
                     /gene="higB"
                     /locus_tag="ACHOQQ_RS20870"
                     /old_locus_tag="pgaptmp_004174"
     CDS             5268..5582
                     /gene="higB"
                     /locus_tag="ACHOQQ_RS20870"
                     /old_locus_tag="pgaptmp_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HGU4472360.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            5579..5995
                     /gene="higA"
                     /locus_tag="ACHOQQ_RS20875"
                     /old_locus_tag="pgaptmp_004175"
     CDS             5579..5995
                     /gene="higA"
                     /locus_tag="ACHOQQ_RS20875"
                     /old_locus_tag="pgaptmp_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGU4472361.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         1567 a         1566 c         1473 g         1393 t
ORIGIN      
        1 tccctcagct tatcccttgc ggctaccggg tcgggcgcaa gggaacgctt cggcgatttt
       61 gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt acataattaa
      121 tagctcacct ttgtctggaa tgagttatct ctagtaatgg cttaatatac agcgaatcat
      181 tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa cgtcatcacc
      241 aggatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa gtcgacaatc
      301 agcatcttga taccgataaa taccagaatc accgccaggc catatttgag catcgagaaa
      361 cgctccgcga cgcccgccag caggaaatac atcgcacgca ggccgaggat tgcaaagagg
      421 tttgaggtca gcacaataaa cgggtcagtg gtcacggcga agatagccgg aatgctatcc
      481 acggcgaaaa tcacgtcgct caattccacc agaatcaata ccagcatcag cggtgtggcg
      541 tacaacaagc cattcttgcg cacaaagaaa tgctcgttgt cgatggtgtc ggtcatgcgc
      601 aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc atcttcatgg
      661 gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata caggatccag
      721 tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag aacaatcgcc
      781 ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga gaaatagctg
      841 aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat cagataaccc
      901 gtgagaaagg ccagtgcctg tggatcggca acggcgcgac cttcggtttg caccagatac
      961 caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga ccaggccgca
     1021 gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag gtcgatagcc
     1081 agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc gacagtattc
     1141 atagaagttc cttacacata aaaaaaacgg ccaacgtcgg aagacgtcag ccgctgcttt
     1201 ttatgcatag acctcgcctt ccggcaaggt ctcacttaca acaaaaaagg aatacgtctg
     1261 cgtattcctt tcgggttgcc cggtgaccgg atgtgatttt tcacacatcg taatgacgat
     1321 cgaccggcaa tgaagttact cccctttgca ggtaacaaag tatgacagac cattcaccca
     1381 gtcaatggtc tgttacatgc cttttacact gctttacggt aacaattatt gttttacgct
     1441 atctgccgga aaaatcaccc ctgtctggcg gcggatctct gtcagcaatt tggcggtgat
     1501 gcgactgacc gccagtcccg gatgatccac cagatgctca tccaccaggg tagcgaacag
     1561 ctctgcttcg tagagcatgg tattaatatg ctgcggctgg gtgagatcct gcatctggct
     1621 gccacgcggc acaaagcata ctttctggca ttcagacagt ttttcaatca ccagcgatcc
     1681 cgcttcgccc tgaatttcgc ttgccaggac agaatcactg acttttgagt gctgcaacgt
     1741 aacgctgaaa tcaccgtaat ccattaccac cacgccgtgg gcatctacgc cgcttgccag
     1801 caaactggcg gttgcctgca cgcttttcgg ctcaccaaat aacgccaccg ccgacgccag
     1861 gcagtaaaag ccgatatcca taattgaacc gttagagaat gccggattaa aggtgttggg
     1921 attctcaccg tccaggtaac gttgataccg cgaggaatac tggcaatagt tgaaaaaaac
     1981 tttccgcagt ttgccgactt tcggcagcgc ctggcgcaac aaatgaaagt tcggcaggct
     2041 ggcggtttta aatgcctcaa acagcaccac ctgattttcc cgcgcacagg caatggcggc
     2101 atccacttcc gccagattcg acgccagtgg tttctcgcaa atcacatgaa ttttatggct
     2161 aaggaaaagt tgtgtctggg aaaagtgcag ggaattcggg ctggcaatat acaccgcgtc
     2221 aatggcatcg ctttctgcca tcgcttccag cgacgtaaac agatgctcga cagaaaaatc
     2281 attggcgaag tgctgggcct gttcaaggct gcgggaatat acggcggtta acttgtattt
     2341 accgctctca tgggcggcct cgacgaactg gcgagtgatc cagttcgtac caatcacagc
     2401 gaaacgtatc ataacgcgtg gatactccat aaagggattc tgccgtcaat gtagcacgcc
     2461 tttttcatct tcgacgtgcg cggatacgca ttatttctgc gctggacaac catgctcact
     2521 aaacggacca ataatgaccg acggcccgag atattttggc tgacgatgga agattttcgc
     2581 gtcaatcacc gcgctgattc tggcgagttt ctcccgtaac tgagtataaa aaccgtgctt
     2641 catattcagg tctggtgcgt taaacccgat agcatcgatg ccgtattgtt ttgccagcca
     2701 gatggcgcgt tgattatgaa attcctgcga gataatggtg atatggtttt ccccaaacac
     2761 ctttttggca cgcactaccg aatccagcgt tgagaatccg gcatagtcgc agaagatcac
     2821 tttcgccggt acgcctttag cgatcaaagc ctgctgcata cctgatgctt catcgtaatt
     2881 tttacgtccg ttatcgccac tgactaacag ccatttcact tttccggcgt ggtacaactc
     2941 tgctgcggta tcaatacgtc gggtaaagta gcgattaccc ggcctggccc ccagcaataa
     3001 gcccacgtta cgcgccggaa cgacgttgac gtcgctccag gtcagctgtt tgctggcgtt
     3061 gaccattacg cgatcggcaa taaaaagggt cgctcctgcc acaagtaaca gtaagcaggc
     3121 aatctttaga gtccggcgag aaaaacaaat acggagaaga aggcgggcaa atgcgcgcag
     3181 catggtcgtt atccttaacg aaagggggga ggtcgatttt aggcgctaaa gaaagaaaag
     3241 ataacgcctg acatcatttt cagattttgt cctgcccaag cgacaactgc gttagccaca
     3301 gccgggtgtc gaactcaagc tggtggtact gcggctccat atggcagcac aattgataga
     3361 acgctttgtt atgctctttc tccttcaggt gcgccaactc gtgcaccacg atcatgcgca
     3421 aaaacggttc cggcgcgttg cgaaacacgg tagcgacgcg gatctctgct tttgctttca
     3481 gcttgccgcc ctgcacacga gaaacagcgg tatgtaaccc gagcgcgttt tttagcacat
     3541 ggatcttatt gtcatacatc actttattga tcggcggggc attacgcaga aactgatttt
     3601 tcagatcctg agtatattgc cagagggctt tatcggtggc gtagtcgtgt gttcccggat
     3661 agcgttttgc cagcacatcg cccagacgct gttcgttaat cagtgttcgc acctgggaaa
     3721 gtagctgctc gggataaccc tgaagataag taagattgct cataaccgcc cacgaaaaag
     3781 agaaaaaggg tatactcacg caccctttgc aggggcaacg tcgaaatttt atcattcagg
     3841 agggccgatg agccacttag acaacggttt ccgttcactg acactacaac gtttcccggc
     3901 gacggatgac gttaacccgc tacaggcgtg ggaagcggcg gatgaatatt tgctgcaaca
     3961 gttggacgac acagaaatcc gcggcccggt gttaatcctg aatgatgctt ttggtgcgtt
     4021 aagctgtgtg ctggcggaac ataagccgta cagcattggc gactcataca tcagcgaact
     4081 ggcgacgcgc gagaatttac gccttaacgg gattgatgaa tcgagcgtga agtttctcga
     4141 cagcaccgcc gactacccgc aacagccggg cgtggtgctg atcaaagtac cgaaaacatt
     4201 ggcattgctg gaacaacaac tgcgtgcgct gcgcaaagtg gtcacgccac aaacacgtat
     4261 tattgccggt gccaaagccc gtgacatcca cacttccacg ctggaactat tcgaaaaagt
     4321 gctcggcccg accaccacca cgctggcatg gaagaaagcg cgcctgatta actgcacttt
     4381 caatgaacca ccgctggccg atgcgccgca gaccgttagc tggaagctgg aaggtactga
     4441 ctggactatc cacaaccatg cgaatgtctt ctcccgcacc gggctggata tcggcgcgcg
     4501 cttctttatg caacatctgc cagagaatct cgaaggggag attgtcgatc tcggttgcgg
     4561 taatggcgtt attggtctga cgctgcttga taaaaacccg caggcgaaag tggtgtttgt
     4621 cgatgaatcg ccgatggcgg ttgcttccag ccgtttgaac gttgaaacca acatgccaga
     4681 ggcgttggat cgctgcgaat ttatgatcaa caacgcgctc tccggcgtgg agcctttccg
     4741 ctttaatgct gtgctctgca acccgccgtt tcatcagcaa catgcgctga ccgataacgt
     4801 cgccagggag atgttccacc acgcccgccg ctgcctgaaa atcaacggcg aactgtatat
     4861 cgttgccaac cgtcacctgg attacttcca taaactgaag aagattttcg gcaactgcac
     4921 caccatcgcc acgaataata aatttgtggt gctgaaagca gtgaagctgg ggcgtcgtcg
     4981 gtaagattga cagctacctg ccagataagg cgtaaacgcc ttatctggca tacgtttagc
     5041 acattacccc caggtcggat aagatgcggc aagcgtcgca tccggcaatg tctgcccagt
     5101 tctggaatcg cgctccataa aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt
     5161 tagcgttgtt caacgtttat tcttccgtca cacagataaa ttcatccgtt gccaatctgt
     5221 caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg cacctgataa
     5281 ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg gagttggtgg
     5341 ctctagggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta aaagcagtat
     5401 tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat gttgggggca
     5461 atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac atacgtgaag
     5521 ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag gggaaaaaat
     5581 gattgcgatt gccgacatct tgcatgcagg agaaaagctg actgctgtgg caccttttct
     5641 ggcgggtatt cagaacgagg aacaatacat ccaggcgctg gaactggtag atcatctgct
     5701 gctcaacgat cctgaaaacc ccttactgga tctggtgtgt gccaaaataa ccgcgtggga
     5761 agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc ctggcggtat
     5821 agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc tgccggaaat
     5881 tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa cgctggaaca
     5941 cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg attaacgcg
//
LOCUS       DAZKFU020000268         5970 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZKFU020000268 DAZKFU020000000
VERSION     DAZKFU020000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.270"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            164..811
                     /gene="fucA"
                     /locus_tag="ACHOQQ_RS20880"
                     /old_locus_tag="pgaptmp_004176"
     CDS             164..811
                     /gene="fucA"
                     /locus_tag="ACHOQQ_RS20880"
                     /old_locus_tag="pgaptmp_004176"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGU4472362.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDTVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            839..1987
                     /gene="fucO"
                     /locus_tag="ACHOQQ_RS20885"
                     /old_locus_tag="pgaptmp_004177"
     CDS             839..1987
                     /gene="fucO"
                     /locus_tag="ACHOQQ_RS20885"
                     /old_locus_tag="pgaptmp_004177"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU4472363.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIICNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2042..2797)
                     /gene="xni"
                     /locus_tag="ACHOQQ_RS20890"
                     /old_locus_tag="pgaptmp_004178"
     CDS             complement(2042..2797)
                     /gene="xni"
                     /locus_tag="ACHOQQ_RS20890"
                     /old_locus_tag="pgaptmp_004178"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGU4472364.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMLALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2909..4276)
                     /gene="sdaB"
                     /locus_tag="ACHOQQ_RS20895"
                     /old_locus_tag="pgaptmp_004179"
     CDS             complement(2909..4276)
                     /gene="sdaB"
                     /locus_tag="ACHOQQ_RS20895"
                     /old_locus_tag="pgaptmp_004179"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGU4472365.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4334..5623)
                     /gene="sdaC"
                     /locus_tag="ACHOQQ_RS20900"
                     /old_locus_tag="pgaptmp_004180"
     CDS             complement(4334..5623)
                     /gene="sdaC"
                     /locus_tag="ACHOQQ_RS20900"
                     /old_locus_tag="pgaptmp_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGU4472366.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1495 a         1481 c         1595 g         1399 t
ORIGIN      
        1 ttgcagttat ttactgtgat tagttgagcc aggtcacaaa atataagctt gtgaatttct
       61 ttcttgttaa tcaggaagaa gatggtgggt aattaaacgg ctaattcaat agtgtgaaag
      121 gaacaacatt attgccctgt tttgaatcag agagaggtag ctaatggaac gaaataaact
      181 tgctcgtcag attattgaca cttgcctgga aatgacccgc ctgggactga accaggggac
      241 agcggggaac gtcagtgtac gttatcagga tgggatgctg attacgccta caggcattcc
      301 atatgaaaaa ctgacggagt cgcatattgt ttttattgat ggcaacggta aacatgagga
      361 aggaaagctc ccctcaagcg agtggcgttt ccatatggcg gcctatcaaa gcagaccgga
      421 tgccaacgcg gttgttcaca atcatgccgt tcattgcaca gcagtttcca ttcttaaccg
      481 accgatcccc gctattcact acatgattgc ggcggctggc ggtaattcta ttccttgcgc
      541 gccttatgcg acctttggaa cacgcgaact ttctgaacat gttgcgctgg ctctcaaaaa
      601 tcgtaaggca actttgttac aacatcatgg gcttatcgct tgtgaggtga atctggaaaa
      661 agcgttatgg ctggcgcatg aagttgaagt gctggcgcaa ctttacctga cgaccctggc
      721 gattacggac acggtgccag tgctgagcga tgaagagatt gccgtagtgc tggagaaatt
      781 caaaacctat gggttacgga ttgaagagta atttcgtaaa gcaacaagga gaaggatgat
      841 ggctaacaga atgattctga acgaaacggc atggtttggt cggggtgctg ttggggcttt
      901 aaccgatgag gtgaaacgcc gtggttatca gaaggcgctg atcgtcaccg ataaaacgct
      961 ggtgcaatgc ggcgtggtgg cgaaagtgac cgataagatg gatgctgcag ggctggcatg
     1021 ggcgatttac gacggcgtag tgcccaaccc aacaattact gtcgtcaaag aagggctcgg
     1081 tgtattccag aatagcggcg cggattacct gatcgctatt ggtggtggtt ctccacagga
     1141 tacttgtaaa gcgattggca ttatctgcaa caacccggag tttgccgatg tgcgtagcct
     1201 ggaaggactt tccccgacca ataaacccag tgtaccgatt ctggcaatcc ccaccacagc
     1261 aggcactgcg gcagaagtga ccattaacta cgtgatcact gacgaagaaa aacggcgcaa
     1321 gtttgtttgc gttgatccgc atgatatccc gcaggtggcg tttattgacg ctgacatgat
     1381 ggatggtatg cctccagcgc tgaaagctgc gacgggtgtc gatgcgctca ctcatgctat
     1441 tgaggggtat attacccgtg gcgcgtgggc gctaaccgat gcactgcaca ttaaagcgat
     1501 tgaaatcatt gctggggcgc tgcgaggatc ggttgctggt gataaggatg ccggagaaga
     1561 aatggcgctc gggcagtatg ttgcgggtat gggcttctcg aatgttgggt tagggttggt
     1621 gcatggtatg gcgcatccac tgggcgcgtt ttacaacact ccacacggtg ttgcgaacgc
     1681 catcctgtta ccgcatgtca tgcgctataa cgctgacttt accggtgaga agtaccgcga
     1741 tatcgcgcgc gttatgggcg tgaaagtgga aggtatgagt ctggaagagg cgcgtaatgc
     1801 cgctgttgaa gcggtgtttg ctctcaaccg tgatgtcggt attccgccac atttgcgtga
     1861 tgttggtgta cgcaaggaag acattccggc actggctcag gcggcactgg atgatgtttg
     1921 taccggtggc aacccgcgtg aagcaacgct tgaggatatt gtagagcttt accataccgc
     1981 ctggtaaatg cgctgatgtg ataatgccgg atacgacgtt tgcggcgtat ccggctcgcc
     2041 gttaccgtac caatcgcaat tgctgtaaat tgccatcgat atgcaaatcg gtttgtaagc
     2101 gggcaatatc gcggcacaga aacgccatct ctttatgggt ttctaatttt ttgcgccact
     2161 tttcggcaac cgcatccaga ttctcatata tcccttccag actctgaaac tcgaccagca
     2221 gctgtgtggc gctttttggt ccgattcccg caacacccgg tacctttgaa ctgctgatcc
     2281 ccgcaagtcc ccagtaatcg ggcaactgct gcggttgaac gccaaattct ttatcgataa
     2341 atggcgcatc cagccaacgt ttctggaagt aatcacgaat acgtaatgtc ggtgaaagta
     2401 actgacagta gcctttatct gtcgaaacaa tcgttgcctg atgcccggcc tgtgtcactt
     2461 tgaccgccag cgtggcggct aagtcatcgg cttcgttgcc gctggttgac cagcacggga
     2521 cgccgcgttg ctcaaaggcg gcgcgtaatg caagcatctc gtcgtgcaac tcttccggca
     2581 ttggcggtcg acccgctttg taatctggta aacgctgatg acgccagccg ctactgcggt
     2641 tttcatcatc aaaaacggcg acagcgtggg ttggctggct gtgcataatg agctgatcga
     2701 gcgcgtgctg gcaggtttcg acacagggcg aaccctgaac ggcatgaatg cgacgaataa
     2761 gattcagtgc atcgacaata agcaaatgaa cagccacgat aaccccctta aagatcaggc
     2821 gataagggta acattcgata ctggaacagg ctatggctgg atgagaaatc gggaagaggc
     2881 ctcgcaaaac gaggcctctg gagagcgatt aatcgcaggc aacgatcttc attgccaggc
     2941 cgccgcgaga ggtttcgcgg tacttggcgt tcatatcttt acctgtttcg tacatggttt
     3001 cgataacttt atcgaggcag acgcgcggct cgctggtacg gcgcagcgcc atacgtgcgg
     3061 cgttcaccgc ttttactgcc gcaatggcgt tacgctcgat gcatggcacc tgtacctgtc
     3121 cggcgaccgg gtcacacgtc agaccgaggt tgtgctccat ggcgatttcc gccgcgatgc
     3181 acacctgcgc cgggcttgcg cctaatagtt ctgccagacc agccgccgcc attgagcacg
     3241 ccacgccaac ttcaccctgg caacccactt cagcaccaga aatcgacgcg ttcatcttat
     3301 aaagagaacc aatggcgctg gctaccagca ggtaacgagc cagtgagtta gcgttcactt
     3361 cgcggataaa cttatcgtag tacgccagca cagccgggat aatcccgcac gcaccgttag
     3421 tcggcgcagt caccacgcga ccgccagcgg cgttctcttc gttcactgcc agtgcaaaca
     3481 tgttgatcca gtcaacaacc gccatcgggt cagtggtggt tttatcctgg ctgaccagca
     3541 tccggcgtag tgccgcagca cggcgtggaa cgcgcagttt gccaggcaac acgccttcgg
     3601 tggaaatacc gcgctcaata ccgccgcgca tgacttccca gacgttcgcc aggtgctgtt
     3661 ccagctcttc tttgctgtgc agcgccagct cgtttttcat catcaggcca gagagtgaca
     3721 gcccggtttc ctgacaatgt ttttgcagat cggctgctga actgtacgga taaggaactt
     3781 caaccggtgc gctatcctgc tggccaaaat gctcttcatc aacgataaag ccaccgccaa
     3841 tagagtagta agtctgactg taaacgactt tatcacccgc cagcgcggta atgcgcatac
     3901 cgttttcatg cagagaaagg ttgtcggcat ggaagttcat gcactgatca accgggaact
     3961 ccacttcatg ctgaccgttt gccagcatca ggcgaccatg agtattcaca tcctgaataa
     4021 aaccagggat ggaatcgata tccacggtat ccggcaggtt acccgccaga cccataatta
     4081 tggcgatatc agtgtggtgg cctttaccgg tcagagagag cgagccgtac acgtcaacca
     4141 ccacgcgggt cacgtcttta agcaggttac gggcaatcag atcgtcggta aattgtttac
     4201 ccgctttcat tggtccaacg gtatgagaac tggaagggcc aatgccgatt ttgaaaatat
     4261 cgaatacgct aatcatagga aatacatcgc gttaaaacgg aggaagcgcc gcccgaaagc
     4321 ggcgcgaaag gacttagctg aacagagagt agaagattgc ggagattgca atcagaccca
     4381 tcacgacaac gaatacgttg ctgatgtgac cgctgtactt acgcattgcc ggtactttct
     4441 gaattgcgta catcggcatc aggaacagga tcatcgcgat gattgggccg cccagggttt
     4501 caatcatacc caggatgctc gggttcaggg tggcaacaat ccaggtcgtt accagcatga
     4561 acagcgcagt gatacggttc agcttgttga tttcgataga cttaccttta ccacgcagag
     4621 atttaatcac cataccgttg aagccttcac gtgcgcccag gtagtggccg aggaaggatt
     4681 tggtgatagc gataatcgcg ataatcggag ccatccacgc gataaccggt gcgttaaagt
     4741 ggttagccag gtaagacaga atcgagatgt tctgctcttt agccgcagcc aggtctgccg
     4801 gagtcaggct cagtacacag ctgaagacga agaacattac ggtcagcacc atcatgatgt
     4861 gtgcgaatgc caggatcttc gagcatttct gttctgccat atcgccgtac tcttcacgct
     4921 tcgcaacggc gaaagaagag atgatcggag agtggttgaa cgagaacacc attaccggaa
     4981 ttgccagcca cagggtcatc cacagaccgt ttccggttgc agatgcagtg tccagagaca
     5041 gcgtttccag tgctgcgccg ttccactgcg ggatcaggta cagagccagc agcatcaata
     5101 cgccaacaaa cgggaacacc agaatactca tcgctttaac gatcatctgc tcaccgaagc
     5161 gaacgatggt catcataccc acgatcagga tcagcgacag aatcgcacgc ggcggtggcg
     5221 tcatacccag ctggtgagac atgaagcttt caactgtatt ggtgattgcc acgctataaa
     5281 ccagcaggat cgggtaaata gcgaagaagt agagcagggt aatcagttta cctgcgccaa
     5341 taccaaagtg ttcttctaca acctcggtga tgtcttcgcc cgggttttta ccagacagta
     5401 cgaagcgagt caggccgcgg tgagcaaaaa acgtcatcgg gaacgcaagg atagccatga
     5461 tgatcagcgg gatcataccg ccaacaccgg cgttgattgg caggaacagc acgcccgcgc
     5521 cgattgccgt gccgtaaagg cccagcatcc acatggtgtc tgtcttgcgc caggcactac
     5581 gagagtcttt cgacgcaatc gtgctggttt gagtcgtttc catctatttc tcctggagga
     5641 atgcaaataa ttcagctttt agccagttca gcccgggaag gtgcctgacc gtaaaaatga
     5701 aatctgtcta tcggcgatgg cttaatcatt taccgccgta ataatttgag ggcggaaaga
     5761 tacacacaag ttatgtatct atcagtgatc ttgatcccaa atacaataat ctacttggaa
     5821 gatagattat cccaacgctt tactctgtaa atcaaaagta aaagagaatt taagggcaat
     5881 atttgcccat taaacgacat cttacttctt ccggggcgcg caatatatgt aggttgaact
     5941 gaaaaaatca atacttttag ttaatcatgg
//
LOCUS       DAZKFU020000269         5958 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZKFU020000269 DAZKFU020000000
VERSION     DAZKFU020000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.271"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            170..898
                     /gene="gadW"
                     /locus_tag="ACHOQQ_RS20905"
                     /old_locus_tag="pgaptmp_004181"
     CDS             170..898
                     /gene="gadW"
                     /locus_tag="ACHOQQ_RS20905"
                     /old_locus_tag="pgaptmp_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGU4472367.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1261..4374)
                     /gene="mdtF"
                     /locus_tag="ACHOQQ_RS20910"
                     /old_locus_tag="pgaptmp_004182"
     CDS             complement(1261..4374)
                     /gene="mdtF"
                     /locus_tag="ACHOQQ_RS20910"
                     /old_locus_tag="pgaptmp_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGU4472368.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGADDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4399..5508)
                     /gene="mdtE"
                     /locus_tag="ACHOQQ_RS20915"
                     /old_locus_tag="pgaptmp_004183"
     CDS             complement(4399..5508)
                     /gene="mdtE"
                     /locus_tag="ACHOQQ_RS20915"
                     /old_locus_tag="pgaptmp_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGU4472369.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5616..5894
                     /locus_tag="ACHOQQ_RS20920"
                     /old_locus_tag="pgaptmp_004184"
     CDS             5616..5894
                     /locus_tag="ACHOQQ_RS20920"
                     /old_locus_tag="pgaptmp_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472370.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5895..>5958)
                     /locus_tag="ACHOQQ_RS20925"
                     /old_locus_tag="pgaptmp_004185"
     CDS             complement(5895..>5958)
                     /locus_tag="ACHOQQ_RS20925"
                     /old_locus_tag="pgaptmp_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4472371.1"
                     /translation="YKLKLRRMSDIVTLGITSYF"
BASE COUNT         1461 a         1489 c         1504 g         1504 t
ORIGIN      
        1 tattcgtgct gcatttactt attatcaatt aactgttatg caaaactact ttgtggataa
       61 attttggtcc taccaaatct ggcagttttt gcgctaagaa acagtctggc atcatttcat
      121 tagtatactg aaattgaaat aatcgcagta tgaaatataa gggataatca tgactcatgt
      181 ctgctcggtg atcctcattc gtcgttcatt cgatatttat catgaacagc aaaaaatatc
      241 gctgcataac gagagtatcc tgctgctgga gaaaaatttg gcagacgatt ttgcgttttg
      301 ttcaccggat acgcgacggc tggatatcga tgagctgaca gtttgccatt acttacaaaa
      361 tattcgtcag ctaccacgca atttagggtt acatagcaaa gaccgtttgt taattaacca
      421 gtcacccccc atgccgctgg tgacggcgat ttttgatagt ttcaatgact ccggggtaaa
      481 ttcgccgata ctgagcaaaa tgctctatct ttcctgttta tcgatgtttt ctcataagaa
      541 agaactgatc cccttacttt tcaatagtat cagtactgtt tcaggaaaag ttgaacgcct
      601 tattagcttt gatatcgcca aacgttggta tctgcgcgat atcgcggaaa gaatgtatac
      661 cagcgagagt ctaatcaaaa aaaagttgca ggatgaaaat acctgtttca gtaaaatatt
      721 actcgcttcc aggatgtcga tggccagacg attactcgag ttacgtcaaa ttcctctgca
      781 tactattgcg gaaaaatgtg gctatagcag tacgtcgtac tttataaaca catttcgaca
      841 atattatggt gtaacgccac atcagtttgc gcaacattcg ccaggtacct tttcctgaca
      901 tattttgcat ttgaatattg gtcaggatct cacacctgct tcatgtgaaa ctcttccctg
      961 atgatttctg ccgggctacc gactagttct ctttcgcagt ttgccaggcg gtataggtat
     1021 tttcaggagg aaaagactcg gcatgtttgg gattattaag ctgacaattc atacgattac
     1081 gggaatgtgg gtcagtattg tgctgttcaa attgatgacg aatggctgga gtggtttcta
     1141 ctttcagtgc tgtgtactaa gcctggtatt cctgacggtt agctggttgt tgagtggtga
     1201 atggttagca ggaaagagta aggctgaacc ttcacgttca accttactct catttacacg
     1261 ttacgctttt ttaaagcggg caaagagatg ttccactaca acgaaaaaga ccggaacaaa
     1321 gtaaattgcc agcactgttg cggcaaacat cccgcccatc acgccggtac ctaccgcgtt
     1381 ttgcgcgcca gaaccggcac catgactgat aaccagcggc agcacgccaa gaataaaggc
     1441 cagagaggtc atcaggattg ggcgtaaacg cattcgtgcc gcttcgatga ttgcctctat
     1501 cggcgttttc ccttctttct gcatcatctc aacggcaaat tcgacaatca ggatggcgtt
     1561 cttggcggaa agcccgatgg tggtcagcaa accaacctgg aagtagacgt cattacttaa
     1621 gccgcgcaga tcggtggcca gtaatgcgcc aacgacgcct aacggaacaa ccaacatcac
     1681 cgagaacgga attgaccagc tctcataaag tgcggcgagg gcgaggaaca ccacgaccag
     1741 tgaaatggca tatagcgcgg gagcctgatt tgaggataac gcttcctgat acgacagtcc
     1801 ggtccatgag tagccgacgc ctgccggaag tttagcgacc aggtctgcca taaatttcat
     1861 tgcgtcaccg gtacttttcc cggccgccgc ttcacctaaa atctccattg acgggatgcc
     1921 gttgtagcgt tccagtcgcg gtgaaccata ggtccattcg gtagacgagt aggcagaaag
     1981 cggtgccatc gtgccagagg cgttgcgtac ataccattgg ttgatgttat ccggcaacat
     2041 acggaacggc gtgcctgcct ggacatacac ttttttcacc cgcccctggt tgaggaagtc
     2101 gttcacgtag ctgctgccga aggcggtgga aattgtctgg ttgatatcag acagcgccac
     2161 acccattgcc tcagctttcg cggcgttgac gttcactttg aacatcggcg tatcttccag
     2221 gccgttcggg cgtaccccgg tgacctgatt cggtgattgc gctgccagtg ataacagctc
     2281 gtttcgtgcc tgggttagtt tttcgtgccc caggttaccg ttgtccagca gttccatatc
     2341 aaaacctgac gcggtaccca gttcagccac cgcgggtaag ttgaacggga agacgacggc
     2401 tttattgata ctgcttagcg caatcattgc ccgctgaatg atcgcggtaa ccgagttttc
     2461 ctcaccgaca cgttcagacc acggcttgag actgataaac gccaggccgt tgttttgccc
     2521 ctggccgctg aagccaaagc cgccaacggt aaacaccgac tggacattat ctttctcttt
     2581 agtcagataa taatccgtca cctgttgcag cactttcgtg gtgttaacca tggtggcacc
     2641 ggaaggtaac tgcgcggtgg tcataaatac cccctgatcc tcttctggta agaaagaggt
     2701 cggcgtgcgc aggaacagca ccgccatccc ggcgcaaatc aggaggtaga ccaccatgta
     2761 gcgaccggta caacgcaaca gtgagcgggt gctatcggta tagtgctgag ttgatttttc
     2821 aaacagcgtg ttgaagcgtg cgaacagggc gttaggtttg tgaccgcctt ccggcgcggc
     2881 tttcagaatg gtagcgcaca gggcaggggt caggctcatt gccacaaata ctgaaagcag
     2941 catggaggag atcagcgtga tggaaaactg gcggtagatc tccccggttg caccgctcat
     3001 aaaggccatc ggcataaaca ctgcggaaag aacaacggca ataccgacca gcgcacgttg
     3061 gatctgcccc atcgatttat gcgtcgcttc cttcggcggt agcttatctt ccgcaatgac
     3121 acgctcgacg ttctccacca ccacgatggc gtcatccacc agtaacccta tcgccagcac
     3181 catcccgaac atagtcaaag tgttgatggt gaaaccgacc gccgacaaga tcgcaaacgt
     3241 cccgagaata accaccggta cggcaatcgt cgggatgatt gtggcacgga aattctgcaa
     3301 aaacagatac atgaccagga agacgaggat gatagcctca accagtgttt tgaaaacttc
     3361 ctgaatagaa atttcgataa acggcgtggt gtcgtaagga taaaccgtct tcagacttgc
     3421 cgggaaatag gctgataagc ggttcagttc ctctttgact gcccgcgagg tatccagggc
     3481 gtttgctccg gtagccagtt tgatggcgat cccggcagca ggtttgccgt tatagcgcgc
     3541 cacggtggaa taatcgtccg ccccaagttc gacgcgggcg acatcacgca gcagcacttg
     3601 cgaaccatct tgctgaactt tcaacaggat tttgccaaat tcttccggcg tttgcagacg
     3661 cgtctgcaca atgatcgagg cgtttagttg ctggtctgcc gcctgtggca tgccacccag
     3721 ttgaccacca gaaatctggt tgttttgcac cttaatctgg gaaataacat cggaaggtac
     3781 cagattgtat ttattgagtt tttgcgggtc cagccagata cgcatggcgt actcggaacc
     3841 aaagagctgt acgctaccaa cgcccgcggt acggcttagc gggtctttga tattagacgc
     3901 tacatagtcc gcgatatcgt actggtttag gctgccgtta tcagaaataa acgccgctac
     3961 catcaggata ttactgctcg atttatcgac gctaatcccc tgctgctgca ctgcttcagg
     4021 taatgaaggc atagcgagtt gcagtttatt ttgcacctga acctgtgcga tatcaggaga
     4081 tgtcccagtc tcgaaggtca gagtgataga ggcattaccc gccgcatcac tggttgaaga
     4141 catgtacatc aggccatcaa gcccattcat attttgctca atcacctgag tgaccgagtc
     4201 ttctaccgtt tgcgcatcgg cacccggata ggtagcgctg acggtaatgg tcggtggcgc
     4261 aatctgcgga tactgcgcaa ccggtaagtt catgatcgcc agaccacctg caagcatcat
     4321 aataatggca agtacccagg caaaaaccgg gcgatcaata aaatagttag ccatgaaagt
     4381 ccccttaagc ctgcaacgtt attgtttcga ttcggtgctg gcgttttcct ggctggagga
     4441 aattgctcgt gctttgatac ccggacgaat gcgttgcaaa ccggaaacga tcacccgatc
     4501 gccagcctgc aagccagagg tgacaaccca ctggtcgccg atggctttgc tggcttcaat
     4561 ttcgcgtagc tgcacgacat cgtctttatc cagaatgagc gccgttgctt taccctgggc
     4621 gttgtgggtg acgccttcct gcggcaccag taatacattc tggcggctac cttcatccac
     4681 taatgccgtg acatacatgc caggcagcaa gtcaccattt gggttgggga aaatcgcccg
     4741 taacgtcacg gagcccgtgg tttcatcaac cgtcgggtcg gagaatttca gcgtgccggt
     4801 ctggctgtaa cgtttaccat tttccagatt gagctgtact ggcgtactgc cctgaacctg
     4861 tttgatttgc ccactggcga cctcttcttt catgcgtaag aaatcctgca ccgactgcgt
     4921 gagatcgaca taaatcgggt ccagacgttg tacggtaacc agcgaatctg cctgattagc
     4981 ggtaacgagt gcgccgacgg tcaccgacga tttcccgctg acgcccgtaa tcggcgaggt
     5041 gacattcgcg tattgcagat tgatcgtcgc ctgttcaaca gccgctttgg cgacggtgac
     5101 attggcttct gcttcattca actgggtgcg cgcggtgtcg taatcctgac ggctaacgta
     5161 gttggtcttc agcaacgatg cctggcggtt aaaggtgatg cgggcattgc tggcggtaga
     5221 gagcgctttc gccagcgagc ctttggcgga gtttagctcg gcctgtaaag gtgcaggatc
     5281 aatctgatac agcgaatcgc cctggttcac tttatcgcct tcgataaagt tgcgtttaat
     5341 gataataccg cccacctggg gacgtatctc ggcaacttca taaggaacgg ttctaccggg
     5401 caattcgctc aacacattga ccgacccagg ggagagtgtg acgacaccga cctcaggcgt
     5461 catggcggcg gcgttttccg ccgatttgtc atcgcaggcg gtgagcatcg cgccgcagaa
     5521 taacaacggt attaacagct ttcttcttct gttcatttta gtccctgaaa attcttgaga
     5581 aataacggtt ctttaaattg cgcgcgagga gagatatgag ggatttacag accagcggca
     5641 tagtggggtt gtcagccagt aaggtgggat acaggcatag tgatcgacat ggtgaggtca
     5701 acgacaaaaa aaatgttgcc gttctgccaa cagttcctgc cagcattcgg gcaacgaagg
     5761 gttctactgg tggatacaca taccagggga ataaagtcgt tttagcctct cctttagtaa
     5821 tcaccggggg taatgaaatc agcatctgca tccctcgtca tgccagccat caatttcagt
     5881 tgcttatgtc ctgactaaaa ataagatgtg atacccaggg tgacgatgtc gctcatacgt
     5941 cttagtttca gcttgtac
//
LOCUS       DAZKFU020000270         5956 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZKFU020000270 DAZKFU020000000
VERSION     DAZKFU020000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.272"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1021
                     /locus_tag="ACHOQQ_RS20930"
                     /old_locus_tag="pgaptmp_004186"
     CDS             <1..1021
                     /locus_tag="ACHOQQ_RS20930"
                     /old_locus_tag="pgaptmp_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HGU4472372.1"
                     /translation="AIMALGLTGCDDKKAETETLPPANSQPAAPAPEAKPTEAPVAKA
                     EAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWLKDFKQGPTGEE
                     RTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKY
                     YTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEERE
                     GKTFHYYSLAVMISAKQINNLISQDEFDAEAAMKKVSELETLVAQAKEADKGGMNFSF
                     INSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMVDD
                     YNRLR"
     gene            complement(1123..2871)
                     /locus_tag="ACHOQQ_RS20935"
                     /old_locus_tag="pgaptmp_004187"
     CDS             complement(1123..2871)
                     /locus_tag="ACHOQQ_RS20935"
                     /old_locus_tag="pgaptmp_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGU4472373.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAETI
                     VGLTGYEGFMALLRQG"
     gene            complement(2871..3941)
                     /locus_tag="ACHOQQ_RS20940"
                     /old_locus_tag="pgaptmp_004188"
     CDS             complement(2871..3941)
                     /locus_tag="ACHOQQ_RS20940"
                     /old_locus_tag="pgaptmp_004188"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4472374.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLMLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            complement(3931..5382)
                     /locus_tag="ACHOQQ_RS20945"
                     /old_locus_tag="pgaptmp_004189"
     CDS             complement(3931..5382)
                     /locus_tag="ACHOQQ_RS20945"
                     /old_locus_tag="pgaptmp_004189"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGU4472375.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5393..5839)
                     /locus_tag="ACHOQQ_RS20950"
                     /old_locus_tag="pgaptmp_004190"
     CDS             complement(5393..5839)
                     /locus_tag="ACHOQQ_RS20950"
                     /old_locus_tag="pgaptmp_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGU4472376.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
BASE COUNT         1525 a         1529 c         1528 g         1374 t
ORIGIN      
        1 cgccatcatg gccctcggtc tgacgggttg tgatgataaa aaagccgaaa cagaaacgct
       61 cccgcctgcc aatagtcaac ctgccgcacc agctcctgaa gcgaaaccta ctgaagctcc
      121 cgttgcaaaa gcagaagcta aacctgaaac gcctgcccaa ccggtggtcg atgaacaagc
      181 ggttttcgac gaaaaaatgg atgtctatat caagtgctac aacaagttac agatcccggt
      241 acagcgcagt ctggcgcgtt atgctgactg gctgaaagat tttaaacagg ggcctaccgg
      301 tgaagagcgt actgtttatg gcatctacgg cattagtgaa tccaacctcg ctgagtgtga
      361 aaaaggcgta aaaagtgctg tggcgttaac gcctgcgctg caacctattg atggcgtagc
      421 ggtgagttat atcgatgctg ccgtggcgtt gggtaatacc attaacgaaa tggataaata
      481 ttacacccag gaaaattata aagacgatgc gtttgcgaaa gggaaaacgc tgcaccagac
      541 attcttaaaa aatctggaag cctttgaacc tgtagccgaa tcttatcatg cggcgattca
      601 ggaaattaat gataagcgcc agcttgcgga gctgaaaaat attgaagaaa gagaagggaa
      661 aacattccac tactactctc tggcagtcat gatttcagcg aaacaaatta ataacctgat
      721 atcgcaagat gagtttgatg cagaagcggc aatgaagaaa gtgtctgaac tggaaacgct
      781 ggtggcgcag gccaaagaag cggataaagg cggcatgaat ttctcgttta ttaattcggc
      841 aggccagtat caactcgagg ctaaaaaata cgttcgccgc atcagagata aagtcccgta
      901 ctctgactgg gataaagagc aacttcagga cgcaaactca agctggatgg tcgaagactc
      961 tttcccgaga gcattacgcg agtacaacga aatggtcgat gattataatc gcctgcgtta
     1021 aacgtttttg tttttgctga gaaatacaag gtcggatgcg gcgtaaacgc cttatccgac
     1081 ctaaacgtcg ttaagccgtg ttagcggtgc atccgacatc attcacccct ggcgaagtaa
     1141 cgccataaac ccttcatacc ccgttagtcc gacaatagtc tccgccggat gctgacacaa
     1201 tacgcttgcc agatagctaa atatttttag ctcatgacgc gcatcggcgg cggcgcaggc
     1261 gatcaagaca tggttaatga cttcgttatt cacctcaact ggttgggcga gggtaataaa
     1321 aaatccacga aaacgtcgct cctgttcgct ccagcaatgt gggatggcga ggcgattaac
     1381 aatcaggttt tcaccttcgc cttcgcgggc gatgatccgt tgcgcttcat cggcggtaat
     1441 atggtgttgt gcgactaatt gcgcacaaat ttgccgggta atatgttgcc agctttcacc
     1501 gcgtacattc gaataatgaa aacttccagg ggcagagaaa aaacgctccg gttgctggcg
     1561 aataaatgcc gtcgccagaa aatgttttat ttgttcgata ccggcagccg taatgatatt
     1621 ttttacggtg atgtaattat ttaccgcgtc atccagtaaa taatggctgt tgttaatgat
     1681 caataacggc tcaatctctt cgcgaatggc aacaagctcg cttaagctac gggcaataat
     1741 cacccgacaa tgtaaaacat cgcgctcaat ggcgagctga ttaatggtgg caatcgcgtt
     1801 ctggtccgag agcaaaatga tcggctggcg ttcgttttga tgtcgttcca gcgcacaggc
     1861 aaaatacaaa ccgatcagat cgctgtcgaa aagaggaata tcgagctgtt ctcgcagtag
     1921 cgtaataaag tgcagactca tatcaaaggc cgcaggccag gcggctttca ggttattcat
     1981 gctgctttgg cggtgctcgg caatccacac cggtgcggca aggcaacgta ataaatgccc
     2041 ggtaatattc tcaaccaact gcgtatcaat aatattcaat gacgcgatgc ctggcaccct
     2101 ttgcagcaac cccagcacat gatcggcaga aattacctgc gcttgctgat gctgtttctc
     2161 tatcaaaccc gtcagggttc ttacagcatt atcaccaaga aacaggccgc tctgttcaac
     2221 gatttgtttt atctcgttac cgggatattg cggccactca tcggtcagtt gattacgcag
     2281 ggcgtaaatc gccagaatca ggctggagag ataatcaccc tgcatcagcg gccagcggtg
     2341 ttggttgtcg cagaccgtgg ataaatgctg aaggttatct cgtgttacgc ccgccagcgg
     2401 tattaaaaac ggatctttgc gtagcaagtt cgccagcaag ataacgcgtt tctcttctgt
     2461 ttcatcaatg aaatggccca aaccggggcg actggccagg cagcaagtac gttcataacg
     2521 ctgtttcagg cggggcagac gctctgctac ccacgtttct ggcaaattaa gcgccgaggc
     2581 gagttgcgca cggggagtga aagtattcag taataacagc gccagcagcc gatcgtcgtt
     2641 atcgtgcttt tgcagcaact gaaaaaaact gcggcgctcg aagatttcca gctgatagcc
     2701 cgcactgcca ctggcgaaaa tgcgggcttt gccgttaagg gtgaagttga gatagtcaat
     2761 atcacgcagg atggtcctgc ctgaaacgcc agtctgttgc gccagctcgc caggcgtgcg
     2821 cggctgttgc tccagcagat cgacaatctt taactggcgt tcgtttagca ttaatccacc
     2881 tgacggaact gactaaacgc gttgactttc tccgcggtta aggtcgtcag aaaaccccgt
     2941 atcagcatga gcagagcatc gtaatcggct tttgaaatca tgccgctgtt ggcgtgcaga
     3001 taacgagttg gcagacacag cgcgacaacc ggacgcccgc cgcccatcac gttgtagcgc
     3061 ccgccatccg tcgctccggt tttcatggtg gaaaattgca gcggtaaacc gttatgtgcg
     3121 gcacagtttt ttaacgctgc taccagtttc tggttgggga agtagcgctt gtcaaacagc
     3181 atcagccccg gcccctggcc cagtttcagc gggtatttaa tgttatcaat gcccggaaca
     3241 tcgcccgcga cggcggtatc cagcacgatc accacatccg gtttagtgtg ttcagccgag
     3301 gtttgtgccc cgcgtagccc aacttcttct tccacgctgc cgacgccgta cagcgtaatt
     3361 tctgggttat ttactgtctg tagtagctcc gccatcatcg cgcagccgat gcggttatcc
     3421 agcgccttgc cgactacttt atcttcgccc cagcaggcaa aattggcctc cgggctaata
     3481 aaattaccaa tttccacgcc gcgttttttc gcttcttcgc gactgttcgc gccaatatca
     3541 atgaacatct catcaaatga cagcggttgt tgcttttgct tttccgttaa cgcatgaggc
     3601 gctacggaac caatcacacc agggattttc actcccttgt gtgtgcgtat tgttacccgg
     3661 tggttgagca tcgactgatt ccaccagccg ccaatggtgg taaagcgcag aaaaccgctc
     3721 tcgtcgatgt gggtgaccat aaagccgact tcatccatat gcccgacaac ggcaactttt
     3781 ggacctttat ttcccttacg ggcaacaaag ctgcccagac catcaaaggt gatctcatta
     3841 acgcaaggtt ccagcgtgtt tatcagaatg tcgcgaactt cctgttcatc gccgctgacg
     3901 gcgctggctt cgcacaactg ttgcagtaac tcaatgttca tggacaagct cctgttgcgc
     3961 ggctttcgct ttgcggcgta accacaatcc tttcaggacg ataatgaatg aaatgttcag
     4021 cgcgaggcca atcgccagca ccagataaaa cgaacccacc ggcgacatca ggccgatcag
     4081 cgggtcaaag atacccaaac caggtgccag acgtttgatt ccgaaagcga tcaccagcac
     4141 gccggtaata ccgcccgcca gcgtattggc ggtaatcatc ggcaacggag ctgccagagc
     4201 gtaaggaata gccggttcgg ttgcgactgt ggcaccgacc acaatggcac tactggcagc
     4261 ctctttttcc tgtcgggtaa acagtttggg cgcaatgaac gtcgcaagac ccgccgcgac
     4321 gggcggcatc agcgcgacaa cgccgacgat ggcgtaccag tcataaatgt gtttttccag
     4381 cagtgagaag cagaagaacc aggcggtttt attgatgggg ccgcccatat cgaacgccag
     4441 catcgcaccg accagaaacg ctgccgcaaa cttcattgat ggaggaatgg tattgaggaa
     4501 gtgcagtagc ccgcccatga ggtcggacat caccggtccg ataacgtagt aagtgagcac
     4561 gccgaaaacc agcagagtaa cgaacgggat cagcattgaa ccgagcagcg gttgtagcgc
     4621 tttgccgaga cggactttac ggaaccagaa gacgaaatag ccaatcgcca gccccagcac
     4681 caccgcgccg aggaatcccg cacccgactg ggtgccgagc agcgctttgt cgttcgccag
     4741 atagcaaacc agaaacgccg gagcgaaagc cggtttatcg gcaatcgacg aggcaatata
     4801 agcgcccatg atgggaatca taaaggtgaa gccaaggtaa ccgatggatt caactaccca
     4861 ggtaaacgac ggcgcgcctt tcgacatatc ggtatacggc aaaccgaact gcaccagcat
     4921 gttggcaagc gccaccagga taccaccgcc gatgacaaac ggcagcgccg cagagacgcc
     4981 cgccatcagg tggctcatca cgctgccgct ttgtacttcc tgtttgccta gcttcacgcc
     5041 gctatctgcg gcaaaaagct gcgaatttgt cggtaattca ctgaaaatct gatcgatatt
     5101 tttcagcgcc tgggagatgg caatctcata aacttttttc ccggcaaacc gtgcgcgatc
     5161 gtcaccgctc aggccacgcc cggtggcgag aatcacgtaa tcggcagcgg caatctcttc
     5221 gctggataag cggttttcaa cgccgctgga cccttgagtt tccactttta tggtatggcc
     5281 gagagaacgg gctttctgtt ccagggcttc cgccaccatg taggtgtgag cgatcccggc
     5341 ggggcagttg gtgatcgcga caatacgtaa ggatgactcc atcgccactt ccttatgagc
     5401 tgagggtttg atttaacaag gcaagcactt ggtcggcgtc gccctgttgc agttggtgaa
     5461 taaaatcctg gtgaataatt ttgcgacaca gtgtgccgat gattttgacc tgatcctctt
     5521 cgccactttg cggcacgccg aggcagatcc agcaattgac gtcttcgccg tcgctggctt
     5581 tccagtcaat agcctgcgct ttgcgggcga ataacacgaa cggttgttta acgcaggcgc
     5641 ttttaccgtg cggcacggcg acaccagaac caaaaccggt ggagtgcatt ttttcgcgca
     5701 gcagcagggt ttgcaggaac tggtgtgagt cggtgataaa accgttttgt aacgccattg
     5761 tcgctaactg cttcagaatg gaataagcgc cattgccctg aatattcagg tcaatacagc
     5821 ttgcagtaag agctgccatt aatcggactc catgaaaaaa tatttcagtt aattgtaata
     5881 gcggggcggg tttgtgatga gcgcggatat gtcgtgaagt cgtgacaata taaagcagag
     5941 tgaaggataa attttg
//
LOCUS       DAZKFU020000271         5915 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZKFU020000271 DAZKFU020000000
VERSION     DAZKFU020000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.273"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..347)
                     /locus_tag="ACHOQQ_RS20955"
                     /old_locus_tag="pgaptmp_004191"
     CDS             complement(<1..347)
                     /locus_tag="ACHOQQ_RS20955"
                     /old_locus_tag="pgaptmp_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HGU4472377.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPE"
     gene            complement(490..993)
                     /gene="cheW"
                     /locus_tag="ACHOQQ_RS20960"
                     /old_locus_tag="pgaptmp_004192"
     CDS             complement(490..993)
                     /gene="cheW"
                     /locus_tag="ACHOQQ_RS20960"
                     /old_locus_tag="pgaptmp_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGU4472378.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1014..2978)
                     /gene="cheA"
                     /locus_tag="ACHOQQ_RS20965"
                     /old_locus_tag="pgaptmp_004193"
     CDS             complement(1014..2978)
                     /gene="cheA"
                     /locus_tag="ACHOQQ_RS20965"
                     /old_locus_tag="pgaptmp_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGU4472379.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFGVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(2983..3909)
                     /gene="motB"
                     /locus_tag="ACHOQQ_RS20970"
                     /old_locus_tag="pgaptmp_004194"
     CDS             complement(2983..3909)
                     /gene="motB"
                     /locus_tag="ACHOQQ_RS20970"
                     /old_locus_tag="pgaptmp_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGU4472380.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGNRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FKTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3906..4793)
                     /gene="motA"
                     /locus_tag="ACHOQQ_RS20975"
                     /old_locus_tag="pgaptmp_004195"
     CDS             complement(3906..4793)
                     /gene="motA"
                     /locus_tag="ACHOQQ_RS20975"
                     /old_locus_tag="pgaptmp_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU4472381.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4920..5498)
                     /gene="flhC"
                     /locus_tag="ACHOQQ_RS20980"
                     /old_locus_tag="pgaptmp_004196"
     CDS             complement(4920..5498)
                     /gene="flhC"
                     /locus_tag="ACHOQQ_RS20980"
                     /old_locus_tag="pgaptmp_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HGU4472382.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5501..5851)
                     /gene="flhD"
                     /locus_tag="ACHOQQ_RS20985"
                     /old_locus_tag="pgaptmp_004197"
     CDS             complement(5501..5851)
                     /gene="flhD"
                     /locus_tag="ACHOQQ_RS20985"
                     /old_locus_tag="pgaptmp_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGU4472383.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         1381 a         1595 c         1443 g         1496 t
ORIGIN      
        1 atttcaggta acggtgccat gcttttgaat tttttataat gagtcgcggc ctgggccaat
       61 gttttcctgg cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc
      121 atcatcatcc gcactgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag
      181 gttgacgtca gctcgccctg ctgttcccgt aattgattgg aaaccacaaa actcttctgg
      241 ctatggtgaa gggaagaaaa aaacagactg ccggaaataa gctgtaacag tgcgaatacc
      301 cccagcacca ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct
      361 gaaaacaagt tgatctcgtt atcggcaagg aagggggaaa ctttattgct gatgccaccc
      421 gccgcgaaat tgaaataaaa cccgatgcgc agatcatcgg gttcatttca attgaggaaa
      481 tcaggagaat tacgccactt ctgacgccgc gctatctaac agcgccatct cttcgctgtt
      541 cagcagtttt tcgatgttca ccagaatcaa catacggtcg cccagtgcgc ccagtccagt
      601 gagatattct gttgaaagcg tcacggcaaa ctctggtgcc ggacgaattt gctccgccgt
      661 caatgaaagc acgtctgaaa cgccgtcaac cacgatgccg accacccgct gtccgagatt
      721 caggacgata actaccgtgt tgtcgttata gtccacatcc acctggctga acttaattcg
      781 taagtcaaca atcggcacaa taacaccgcg cagattcgtg acgcctttga taaacgccgg
      841 cgtgttcgca atccgtgtta cctgatcgta gccacggatc tcctgcactt tcaggatatc
      901 aataccgtac tcttcatcac caagggtaaa taccagaaat tcctggcctg acggctcgct
      961 ggccagcttt gttacattcg tcataccggt catattgtta cctttttact cattcaggcg
     1021 gcggtgttcg ccatacgttg ttcgcggttt atcgcctgca aggcggagac atcaacaatc
     1081 agtgccacgc tgccgtcgcc aagaatggtc gcagcagaaa tgccggggac tttgcgatag
     1141 ttactttcaa ggtttttaac cacaacctgg tgttgaccaa ttaattgatc caccagcaag
     1201 gcgtagcggc gaccgccact ttgtaagatc accacaattc cctgggtagc ttcggttttc
     1261 gcgcccgcga cgttgaacac tttccacagt tcgacgatgg gcagatattc accccgcact
     1321 tccagcaccc gctcgccgcc ggccagtgga tggagatcgg cttcacgggg ttgcagtgat
     1381 tccataacag cattcagcgg caaaatgaaa acttcatccg caacgcgtac ggacatgccg
     1441 tcgaggatgg ccagcgtcag cggcagtaaa atgcggatcg tcgtgccagt accctgcttc
     1501 gactggattt cgacatgacc gcccatctcc tggatattac gtttaacgac gtccatgccg
     1561 acgccgcgcc cggagacgtc ggtgacctgc tctgccgtgg agaagcctgg tgcaaatatc
     1621 agcatcgcga cttcgtcgtc gctcatgttt tcgctgacag tcaaaccttg cgaggccgct
     1681 tttgccagaa ttcgctcacg gtttagcccc gccccatcgt cggtcacttc aatgcaaatg
     1741 ttgccgccct gatgttcggc agacagaatt aaatttccga cgctgttttt acctgcggcg
     1801 agccgttttt ctggcagttc aataccgtga tcgaggctat tgcgtaccag gtgggtcagc
     1861 gggtcgataa tgcgttctat caggctcttg tcgagctcgg tggaactgcc caccagcgtc
     1921 agttctacct gcttgccgag ttttcccgcc agatcacgca ccagccgggg atagcgacta
     1981 aagacatatt ccatcggcat catgcgaatc gacatcactg attcctgcaa atcacgggcg
     2041 ttacgttgta actgccccat gctggtaatc aaatcaccat gattaaccgg gtccagttcg
     2101 ctggaacgct gggcaagcat ggactgggtg ataaccagct cgccgacgag gttaattaat
     2161 tgatcaacct tttctaccgc tacacggatg ctggtggatt cactgctacg cgtcgttttt
     2221 tcccgctcca cgcgaccggt tggcgcttgt tcggctgcca gtttaagcac tggtggggtg
     2281 gatatttttg gcgagacttc tactgtttca aaggtaatct gatcggcttc aatcacaaaa
     2341 cagagtaccg ctgtgatgtc atcttcggcg atatcgcccg gtaatattgc cgagagagaa
     2401 tccgcccctt tcaccacgtc agttaacgtt gtcagatgcc ccagctcttc ttccagcagg
     2461 tcgacttcgc cggccttcag gcgcgaaagg ataattcgtc gcggcaactg actgcgactc
     2521 tgctcatctt gcggttcact tttggcaacc acacttaatc gggtcactgc ggatggcgtt
     2581 tcgcctttcg cttctaatgc cagttgacgc aaggcctggc agatataatc gaagctggcg
     2641 gcatccggct cttgcgactg tttataagcg tcgagctgtt cttgcatgat gtccttcgtt
     2701 tccaaaaaca gattgataat gtcggtgttg agttgcatct cacctcgtct ggcttcatcg
     2761 agcaggtttt ccatcagatg cgtggtttcc tgcaaaacgc cgaagccaaa agttcctgcc
     2821 cctcctttga tcgagtgggc agcccgaaag atggcattca attgttcggc atctggcgct
     2881 tccggctgca aaaccagcag atgctgctcc atatcagcca acagttcgtc cgcttcatca
     2941 aaaaatgtct gataaaaatc gcttatatcc atgctcacgc tgtcacctcg gttcggctga
     3001 tggcattgtg ggaacactga cctgtggtgc aacctcaggt ttttccaggg cgcttactgg
     3061 ctcattctgg ctttcggcgt tttcatgcaa aatggcctgt tcggcttgtt tgttcagaac
     3121 cagcaggctg atgcgacggt tgacggcatc atcaggtccg cgatcgctta agcgcatcgt
     3181 tgccgccatg ccgacgacac gtaacacttt gccgctatcc aacccgccga ccatcagttc
     3241 gcggcgggat gcattggccc gatcggcaga aagctcccag ttgctgtaac ctttctcacc
     3301 gctggcgtag ggaaaatcat cggtatgacc tgaaaggcta atacggttgg gaataccgtt
     3361 cagtacaggc gcgatggcgc gcagaatgtc gcgcatatag ggttcgacat cggcactgcc
     3421 ggttttaaac atcgggcgat tctggctatc gatgatctga atacgtagac cttcctggac
     3481 cagatcgatt ttgagatgtg gacgtaacgc ccgcagtttc ggatcggact ctatcaactg
     3541 gtcgagatca ccccgcaatt tccgcaatcg actttgctcc atgcgttttt tcaactcttc
     3601 gatgttcggc tgcttattca cttccccctg gctttgggtg taatcatcac caccgccggg
     3661 gattgggctt tcactattag aaatgcgatt gccgcccgta accgctgtcg ccagtggagt
     3721 ccggaagtac tccgcaatct gaatcaactc ttttgggctg gagatggaga tcagccacat
     3781 caccagaaaa aaggccatca tcgcagtcat aaagtcggca taagcaatct tccacgatcc
     3841 atgtgctgcc ccgtggcttt tggctttgcg tcgtttgacg acaataatcg gatgcgcttg
     3901 attcttcatg cttcctcggt tgtcgtctgt tgttgcggat ttttcaccgc acgcacatgc
     3961 tcttccagtt caataaacga cggacgttcg ctggaataga gcgttttgcg accaaactca
     4021 acggcgatag gcggtgcgta accgttcaga ttagaaagca gtgtgacttt gacgcactgc
     4081 atcattttgc tggtttcggc gcttttctga cgtaaaacac tcgctaatgg ggaaataaat
     4141 ccgtaagcca ataaaatgcc gaggaaagtc cccaccatcg catgtgcgat aagcgccccc
     4201 agctcggcgg caggacgatc ggctgaacct aacgcgtgaa cgacccccat tacagccgcg
     4261 acaataccaa acgccggaag tgagtccccg accagcgcca gactgttcgc cgggacttct
     4321 gcctcgcttt cgtgcgtctc aatctcttca tccatcaggg cttcgatttc gaaggtgttc
     4381 atgtgaccgc tgataatcag gcgcagataa tcgacgataa aatcaagcat gacgctatcc
     4441 gcgaggatgc gtgggtagct ggcgaagatc tcgctctcac ggggattttc aatatcacgt
     4501 tccagtgaaa acatccccat ctgccgcgat ttcgccatca accgataaag cagagccagc
     4561 agatccatat acattgcttt ggtgtatttg gagcgacgaa acagcaacgg cagcgctttc
     4621 agcgtgcctt taatcgcttt gccattattg ccgacgataa acgacccaat ccctgcaccg
     4681 gcaataatca ccagttcagc gggttgatag agtgctccaa ggcttccacc ggtcatcaaa
     4741 taaccgccga aaactgtacc gagaacaacc aggtaaccta ataagataag cacgacatca
     4801 tccttccact gttgaccatg acaggatgtt cagtcgtcag gcgttaacgc gcgattgggg
     4861 caaaaaaaag cagcggtacg tcgttaccgc tgctggaatg ttgcgccaca tcgtatcagt
     4921 taaacagcct gtactctctg ttcatccagc agttgtggga taatatcggc aggattctgg
     4981 gaaagtttac gtctttttac tgcccgggat ggcggttgac ataagctgca ggcaaagctg
     5041 ccaacaggct ggtgagcgtg ggtaataaaa ttgccgccgc agcagttgca gctggaaagt
     5101 tgcagtaatc cactttcaac aaaccgcacc aatgtccagg cacgggttaa tgccagcagc
     5161 ggtccttctt ctgcttgcgg gcactgttca aggtataaac ggtaggcttt gatcaccgca
     5221 tcgacgccat tacacaaacc ggttttcagt aaaaactgcc atgcattaca gaacatcgaa
     5281 gcatgaacgt tttgttccca ggtcataaac cagtcggttg agaatggcag catgcctttc
     5341 ggcggtgggc ttccgcgcag ttctttataa agttttatca ggcgtccgcg acttaactgt
     5401 gtttcgcttt ccagcatctg caaacgagcg cccagggtga tcaattccat tgccagctga
     5461 atatcccgcg cttcctgaac aatgcttttt tcactcatga tcaggccctt ttcttgcgca
     5521 gcgcttcttc aggctgatta acatcattca gcaagcgtgt tgagagcatg atgccggtat
     5581 gaatttgctg gagatcgtca acgcgggaat cttgcgtcaa ctgagtaatc gtctggtggc
     5641 tgtcaaaacg gaagtgacaa accagctgat tggtttctgc cagcttaacc atttgcggaa
     5701 gagtcagtgc cgctaatgtt gtcgccattt cttcatttat gccgagacga aacatagcgg
     5761 acgctttgtc ctgaacaatc agacgctgtg caagtagtaa atatgacaag ttgatgtcat
     5821 aaatgtgttt cagcaactcg gaggtatgca ttattcccac ccagaataac caactttatt
     5881 tttatgcggt ttcaccgcac cccgtgatgt cgccg
//
LOCUS       DAZKFU020000272         5821 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZKFU020000272 DAZKFU020000000
VERSION     DAZKFU020000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.274"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..323
                     /locus_tag="ACHOQQ_RS20990"
                     /old_locus_tag="pgaptmp_004198"
     CDS             <1..323
                     /locus_tag="ACHOQQ_RS20990"
                     /old_locus_tag="pgaptmp_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4472384.1"
                     /translation="SKSVDTRSIYRELGATLSYNMRLGNGMEVEPWLKAAVRKEFVDD
                     NRVKVNSDGNFVNDLSGRRGIYQAGIKASFSSTLSGHLGVGYSNGAGVESPWNAVAGV
                     NWSF"
     gene            complement(695..961)
                     /gene="minE"
                     /locus_tag="ACHOQQ_RS20995"
                     /old_locus_tag="pgaptmp_004199"
     CDS             complement(695..961)
                     /gene="minE"
                     /locus_tag="ACHOQQ_RS20995"
                     /old_locus_tag="pgaptmp_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGU4472385.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(965..1777)
                     /gene="minD"
                     /locus_tag="ACHOQQ_RS21000"
                     /old_locus_tag="pgaptmp_004200"
     CDS             complement(965..1777)
                     /gene="minD"
                     /locus_tag="ACHOQQ_RS21000"
                     /old_locus_tag="pgaptmp_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGU4472386.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1801..2496)
                     /gene="minC"
                     /locus_tag="ACHOQQ_RS21005"
                     /old_locus_tag="pgaptmp_004201"
     CDS             complement(1801..2496)
                     /gene="minC"
                     /locus_tag="ACHOQQ_RS21005"
                     /old_locus_tag="pgaptmp_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGU4472387.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3016..3384
                     /locus_tag="ACHOQQ_RS21010"
                     /old_locus_tag="pgaptmp_004202"
     CDS             3016..3384
                     /locus_tag="ACHOQQ_RS21010"
                     /old_locus_tag="pgaptmp_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGU4472388.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3504..3905)
                     /gene="pliG"
                     /locus_tag="ACHOQQ_RS21015"
                     /old_locus_tag="pgaptmp_004203"
     CDS             complement(3504..3905)
                     /gene="pliG"
                     /locus_tag="ACHOQQ_RS21015"
                     /old_locus_tag="pgaptmp_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGU4472389.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            3976..4134
                     /locus_tag="ACHOQQ_RS21020"
                     /old_locus_tag="pgaptmp_004204"
     CDS             3976..4134
                     /locus_tag="ACHOQQ_RS21020"
                     /old_locus_tag="pgaptmp_004204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472390.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            4146..4439
                     /locus_tag="ACHOQQ_RS21025"
                     /old_locus_tag="pgaptmp_004205"
     CDS             4146..4439
                     /locus_tag="ACHOQQ_RS21025"
                     /old_locus_tag="pgaptmp_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGU4472391.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4511..5170
                     /locus_tag="ACHOQQ_RS21030"
                     /old_locus_tag="pgaptmp_004206"
     CDS             4511..5170
                     /locus_tag="ACHOQQ_RS21030"
                     /old_locus_tag="pgaptmp_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU4472392.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5262..5708
                     /locus_tag="ACHOQQ_RS21035"
                     /old_locus_tag="pgaptmp_004207"
     CDS             5262..5708
                     /locus_tag="ACHOQQ_RS21035"
                     /old_locus_tag="pgaptmp_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGU4472393.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT         1487 a         1300 c         1436 g         1598 t
ORIGIN      
        1 aatcgaaatc agtcgatacc cgcagtatat atcgtgaact gggcgcaacg ctgagttaca
       61 acatgcgtct gggaaacggt atggaagttg agccgtggct gaaggcggct gtgcgcaaag
      121 aatttgtcga tgataatcgg gtgaaagtga atagtgacgg taattttgtc aatgatttgt
      181 cgggcagacg tggaatatac caggcaggta ttaaagcctc attcagcagt acgttaagcg
      241 ggcatctcgg ggtggggtat agcaacggtg ccggtgtgga atccccgtgg aacgcggtgg
      301 ctggtgtgaa ctggtcgttc tgaccaccaa cgattaaact gcgcttcggc gcagttttcg
      361 tttacaggat gttgaaagag aaaattctgg ggcaaaaaaa gcccgccagt tacggcggga
      421 aacctcatcc tatgggagaa caatgaataa tgaaattgcg gggttatcat ctcccagtat
      481 atccacacta acaataaggt tatttactca accaggcata aacattttgt tctgtgcgtg
      541 ggaacagcct taaggtgtaa agagggaggt ggaaatagca atgaggagta tcagcaagaa
      601 tactcgccgc tttaccacaa cgtcgatgag agggatgaaa aactcaaggc agagatgact
      661 ctgccttgaa gataaatgcg cttttacagc gggcctattt cagctcttct gcttccggca
      721 aggtcacgtt cagctcaaga atagaaatat cgccatcttt ttgctcaagc tgtacggtta
      781 ccatctcagg atcaatttgt acgtatttac aaatgacctc aagaatatct ttacgcaact
      841 gcggcagata atgcggttct gcatcgctgc gacggcgttc agcaacaata atctgcagcc
      901 gttcttttgc aatgttggct gtgtttttct tccgcgagag aaagaaatcg agtaatgcca
      961 taacttatcc tccgaacaag cgtttgagga agcctttctt ctcttcttca atgaagcgga
     1021 aaggacgttc ttctcccaac agacgttcta cggtatctgc gtaggcttta cccgcatcgg
     1081 cgttaatgtc gagaatgacc ggttcaccct ggttagaggc gcgcaatact gattgatcct
     1141 ctgggatcac gccgacgagt ttgatgcgca ggatctccag cacatcttcc atgctcagca
     1201 tgtcacctct gcttacgcgg cctgggttat agcgcgttaa cagcaggtgc tctttaatag
     1261 gctcttcgcc attttctgcg cggcgtgatt ttgacgccag aatgcctaaa atacggtcag
     1321 agtcgcgtac tgaggagact tccgggttgg tggtaataat ggcttcgtct gcaaaataga
     1381 gtgccattaa cgcaccggtt tcaatccctg ccggggagtc acaaacgata aattcaaaat
     1441 ccatcgcttt cagatcatca agaactttag cgaccccttc acgggtgagg gcatctttat
     1501 cgcgtgtttg cgatgccggc agaatataga gattttcagt acgcttatct ttaattaacg
     1561 cctgatttag cgttgcatcg ccctgaatga cgttgacgaa atcgtaaacg acccggcgtt
     1621 cgcaacccat aatcaggtcg agattacgca ggccgatatc aaaatctatc acgacagttt
     1681 tctttccctt ctgggccaaa ccagtggcga tggccgcgct ggaggttgtc ttaccaacac
     1741 cccctttgcc cgaagtaaca acaataatgc gtgccataga aattccttgt taaaaaggga
     1801 tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac tgcagtcgcg ccgctttgcc
     1861 ataaaattct gctgggattt gatcactcag ccagtattca cctgcgatgg acaccagttc
     1921 cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca ccacttgccc ctgccagcgc
     1981 acgaccgcgc atcatgccat agacatgaat gttaccatcg gcaattaatt cggccccagc
     2041 gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca taaatacgct gaccggaacg
     2101 caccggggta tctattaaac gcgtttttgt gaccggcgtt gtattttgcg ctggagcctg
     2161 cggtgcggga gctggacgtg gcgctttttc ctttccttcc gtcaggatag gcagccccat
     2221 tttttcaatt tcggctttaa gttgcgcatc tttgcagcca cttacgccaa taacccgcaa
     2281 accggttgcc gaaaccgcct tatgcatcgc tgaccagttt accgggtctt ccagtgcact
     2341 gacgttgagt acaacggggg catgttttaa aaatgcgggg gcctgagcga ttttgtcttc
     2401 cagcgcctga tggataacct taggttctgc ctcatgcaga tgaaccacag ataaagtgaa
     2461 gctactgcct ttaagctcga ttggcgtgtt tgacatcctg gccttactca attagctatt
     2521 aatcatcgcc agcgcgcgat gatgttccga agactataag gcatgttata gtctggatta
     2581 tattgaggca agtcaccctc ccatttattc agagtaaaag tctattctgt gataaatggc
     2641 gctgattcat agcttaaaaa atacccttgt caatcaaccc attgccgtcg tacttttgat
     2701 tgttcttatt tacgcttcct ttttccgcac cctaatcgtg acagcatctt tagacctgtt
     2761 taaatatcca gtattgaggc aagtgtattt tttcaccatg ccaggctttc ttaactttcc
     2821 attttttgca gcatcattaa agaacagata cccgttatct taatatccgc cacagaattg
     2881 ctctatcata gaaaccgcag aagacgtctg atacttctgt caggtcatcg taactcgtcc
     2941 aggtcagcgg ttttgattac ggattgatga tgaaatgagc ataagtgttg tacggttgga
     3001 ttatcaggat gtgctatgaa tatgatgcgt attttttata tcggattgtc aggtgtgggg
     3061 atgatgttct catcaatggc gtctggacat gacgctggcg gacttcaatc tccggcgtgc
     3121 ggcgttgttt gcgacccgta tatttgtgtg aactcagatg gcatttcccc agagttaaca
     3181 aggaaatatc tcggcgaaaa agccgctgaa aacttacaat cattacaagg ctacgatccc
     3241 agcgaattta cattcgctaa cggtgtattt tgcgatgtta aagaaaaatt atgtcgtgat
     3301 gatcgttatt ttggtgtgga tggtaagcgg agtggaaaaa tcaatcaaac caccacaaag
     3361 atgttattta tgtgtcgtga gtgatgcctt gtttacatta tgttctattc ggcattgtct
     3421 gctattcgtc attaacctct tgagcgacag agaggagtgg ggcgttaacg tttatgacgg
     3481 ggagagtccc cgtcggttga catttactta atttgaatat cgacgttata ttttttagtt
     3541 ttgtttttac gggcgtcgtt tcgggtttgc agcactcgca gctcatattt gccagaagca
     3601 gggagtgagt attgtccgtg actatcgagt tctggggaat atctggataa atcaacagaa
     3661 tcatcgattc ctggcccgaa gaggtaagta tcagcacctt cattagagat actcacatgc
     3721 actttctgac cttttttggc atagaaggta tatgtatcgt aatcgtatcc ctttatttcg
     3781 ccggagtact gagcgctgct atggcctttt ctgaactcaa cattgacatt cttacccgca
     3841 gcaaagctcg tggaggttag cagtgcaagc agcaatacag ccttactgat gctcttgatt
     3901 ttcattggta gtctcctgtt cagaatgagt acgatgaact gttataatat aacaatccct
     3961 tacgggagaa ttccagtggc tttcctgcgt tcggtaaaat cccgtcgcgg catttttgcc
     4021 ggggggatta cttataaagg ggcatggact caacaaaccg tagaaatcta cagctggctg
     4081 gcttgtggcg cgggtttcat tgatgttagc ttatgccaaa acctggcatc ttaaagagta
     4141 aaagtatgtt ttgtgtgatt tatcgaagca gcaagcgtga ccagacctat ttatatgtcg
     4201 aaaaaaaaga cgatttttcg cgtgttcctg aggaactgat gaaaggtttt ggtcagcctc
     4261 agttagcgat gattctgccg ctggatgggc ggaagaaact ggtcaatgcc gatattgaaa
     4321 aggttaaaca ggcattaacc gagcaaggtt actatttgca gttaccgcca ccacccgaag
     4381 atttgctgaa gcaacatctt tccgtcatgg ggcagaaaac agacgacact aacaaataac
     4441 cgatatccgg cggtggcatt atctttgtcg gcgcgggttt tcatatccac gataaggtga
     4501 ggggaacgtt atgtatcaac atcacaactg gcaaggtgcg ctgctggatt atccggtgag
     4561 taaagtagtc tgtgttggca gtaactatgc caaacatatt aaagagatgg gcagcgcagt
     4621 gcccgaagag ccagtgctgt ttattaaacc agaaacggca ctgtgcgatc tgcggcagcc
     4681 attggcgatc ccatccgatt tcggttcagt tcatcatgaa gtcgaactgg cggtgttgat
     4741 tggcgcgacg ctgcgtcagg ctacggaaga gcatgtccgc aaagccattg ccggttatgg
     4801 cgtggcgctc gatctgacat tgcgtgatgt tcagggaaaa atgaagaaag ccgggcagcc
     4861 gtgggaaaag gctaaagcgt ttgataactc ttgtccgctt tccgggttta ttcccgcggc
     4921 ggaatttacc ggcgacccgc aaaatacaac gctgggcctg agcgtaaacg gcgaacaacg
     4981 ccagcaaggt acgactgcgg acatgatcca taaaatcgtt ccgctgatcg cttatatgag
     5041 caagtttttt accctcaagg ccggtgacgt tgtgctgaca ggcacgcctg atggcgtcgg
     5101 cccgttgcaa agcggtgatg agctgacagt cactttcgat gggcattctt tgacaactcg
     5161 cgttttgtaa tactttttgc cgcctgaaag cggcggcaaa acttgcatcg ctgtgccaga
     5221 ctggttataa ggtgcgtttt aacgtaatgg cggaacacct gatgagcgat gtacctttct
     5281 ggcaaagtaa aaccctggac gaaatgagcg atgcggaatg ggagtcgttg tgtgatggtt
     5341 gcggtcagtg ttgcctgcat aaactgatgg atgaagacac cgacgaaatc tacttcacta
     5401 acgtcgcctg tcgccagctc aatattaaaa cctgtcaatg tcggaactac gaacgtcgtt
     5461 tcgagtttga acccgactgc attaaattaa cccgtgaaaa tctgccaaca ttcgaatggc
     5521 tgccaatgac ctgcgcttat cgtttgctgg cggaaggtaa agatttacct gcgtggcatc
     5581 cgctacttac tggttcgaaa gcggcaatgc atggtgaacg tatctctgtg cgtcatatcg
     5641 cagtgaaaga atcagaagtg attgactggc aggatcatat cttaaataaa cctgactggg
     5701 cgcagtgata aatttaatag atgttgatgg gggctttctt aatatacgtc tggatgctcg
     5761 ccatttcgta gccctgtttt tgaaagcgct ctgtcataat gagagttacc cgatacctta
     5821 t
//
LOCUS       DAZKFU020000273         5793 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZKFU020000273 DAZKFU020000000
VERSION     DAZKFU020000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.275"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(75..1277)
                     /gene="ubiI"
                     /locus_tag="ACHOQQ_RS21040"
                     /old_locus_tag="pgaptmp_004208"
                     /gene_synonym="visC"
     CDS             complement(75..1277)
                     /gene="ubiI"
                     /locus_tag="ACHOQQ_RS21040"
                     /old_locus_tag="pgaptmp_004208"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGU4472394.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1300..2478)
                     /gene="ubiH"
                     /locus_tag="ACHOQQ_RS21045"
                     /old_locus_tag="pgaptmp_004209"
                     /gene_synonym="visB"
     CDS             complement(1300..2478)
                     /gene="ubiH"
                     /locus_tag="ACHOQQ_RS21045"
                     /old_locus_tag="pgaptmp_004209"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGU4472395.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLECGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2475..3800)
                     /gene="pepP"
                     /locus_tag="ACHOQQ_RS21050"
                     /old_locus_tag="pgaptmp_004210"
     CDS             complement(2475..3800)
                     /gene="pepP"
                     /locus_tag="ACHOQQ_RS21050"
                     /old_locus_tag="pgaptmp_004210"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGU4472396.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3826..4404)
                     /gene="ygfB"
                     /locus_tag="ACHOQQ_RS21055"
                     /old_locus_tag="pgaptmp_004211"
     CDS             complement(3826..4404)
                     /gene="ygfB"
                     /locus_tag="ACHOQQ_RS21055"
                     /old_locus_tag="pgaptmp_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGU4472397.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4572..4901
                     /gene="zapA"
                     /locus_tag="ACHOQQ_RS21060"
                     /old_locus_tag="pgaptmp_004212"
     CDS             4572..4901
                     /gene="zapA"
                     /locus_tag="ACHOQQ_RS21060"
                     /old_locus_tag="pgaptmp_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4472398.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4943..5126
                     /gene="ssrS"
                     /locus_tag="ACHOQQ_RS21065"
                     /old_locus_tag="pgaptmp_004213"
     ncRNA           4943..5126
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHOQQ_RS21065"
                     /old_locus_tag="pgaptmp_004213"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5147..5749
                     /locus_tag="ACHOQQ_RS21070"
                     /old_locus_tag="pgaptmp_004214"
     CDS             5147..5749
                     /locus_tag="ACHOQQ_RS21070"
                     /old_locus_tag="pgaptmp_004214"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGU4472399.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
BASE COUNT         1359 a         1635 c         1496 g         1303 t
ORIGIN      
        1 aggaaaatct cccggggaaa aaaccgggag atgaaagtgt gatgggtatc aaataaacaa
       61 cagaggagaa atttttaacg cagccattca ggcaaatcgt ttaatcccat tgcctggcgg
      121 ataagttgcg gcttaacgcc aggaagcgtg tcggccagtt tcaaaccaat atcacgcagc
      181 agttttttcg ccggattagc gccggaaaag agatcgcgaa aaccctgcat accagccagc
      241 atcagcgccg cgctgtgctt gcggctacgt tcatagcggc gcaggtaaat gtactgcccg
      301 atgtctttac cctgacgatg caaccgtttc agttcagcaa tcagctctgc agcatccata
      361 aagccgagat ttaccccctg ccccgccagc gggtgaatgg tatgcgcggc gtcgcccacc
      421 agcgccagac gatgcgcggc aaactggcgc gcataacgcc ccgtcagtgg gaacacctga
      481 cgcgcgctct caaccttgca taagccaagg cgattatcaa aagcgatatt taacgcgcga
      541 ttaaattcgt cttcacttgc ctgctgcatc cgctgcgctt cctctggcga cagtgaccag
      601 acaatcgagc aaagatgcgg atcgctaagc ggtaaaaagg ccagaatgcc ttcgccatgg
      661 aaaacctgcc gcgccaccgc atcatgcggt tcttccgtgc gtatggtcgc taccagcgcg
      721 tgatgctgat aatcccagaa agtcagcgga atatcggctt tgttgcgcaa ccaggaatta
      781 gcgccgtccg cgccaatcac cagacgcgcc gttaacatac tgccatcttt cagcgtcagg
      841 aaggtttcgt tttctcccca ggcgacctgc tgtaattctg cgggggccaa cagagtgata
      901 tctgacgact gctgcgcttt gttccacagc gcgtagtgaa tcactgagtt ttcaacgata
      961 tgcccaagat ggctatagcc catgctttga tcgtcaaacg aaatgtgacc aaaactgtct
     1021 ttgtcccaca cttccatacc gtgatagcaa ctggccctac gagagagaat gtcttgccag
     1081 acgccaagac gggtgagtaa tttttcgctg gcggcattga tagccgaaac gcgcagttgt
     1141 ggtggtgcat ccaccgccag aggttccggt acgcgctgct ccagtacggc aacgcgtaag
     1201 ccgctcccct gtaagccgca ggcaaccgcc agccccacca tgccgccgcc aacaatggct
     1261 acatcaacac tttgcattgt ttattcctta aaaccgcctt caacgcgcca cccaaccgag
     1321 ggtgcgctgc gccagcacat cgcgtgccgg ggtgaataat tccatcgtca tcagcccgat
     1381 gttgcgcccg acaaccagtg gcgtccaacg gttggcaaaa agatgtacaa ggctgtccgt
     1441 gacgccaatg gttgcttcgc gatcgctctg tcgacgctgc tgataacgac acaatacgcc
     1501 gtaatcaccc atgtcttctc cgcgctcctg cgcctgagtc agggtttccg caagactcat
     1561 gacatctcgc ataccgaggt tgaacccttg cccggcaatc gggtgcaggg tttgcgccgc
     1621 attgcccacc agcacggtac gatgggtaat agatctggcg gcgtgggtta acgccagcgg
     1681 ataagcactg cgtttaccag cgtgggtaat tttcccaagt cgccagccaa aggccgactg
     1741 gagttcacgg caaaacttct cgtcgctcca cgacagcacc tcttcgcgcc gttccagtgg
     1801 atgacaccag accagcgaac agcgtccgtc agacatcggc aacatcgcca gcgggccatg
     1861 ttgcgtaaag cgttcaaaag cgcgcccttc atgcgcaacg gaagtcgcaa cgttggcaat
     1921 cacagccagt tgttcgtaag gctcctgctg ccagtcaacg ccgcacacag tggctaacgc
     1981 tgaatgggtg ccatcagcgg ctaccagcac gcggcccgtc agcgtctcgc cacactccag
     2041 cgtcacttca acatgactct gagtacgggc aacgttagcc acgcgatccg ggcaatgcag
     2101 cgttacgcca ggtgctttgc gcagcaatgc aaacagccgt tgcccgacat tgtgcaattc
     2161 gacaacctgc cccagcgccg ccagttggta atcttctgcg gcgagggtga cgaatccggc
     2221 atgaccacga tcgctgacat gcacggtagt gatggcggtt gcgcaatccg ccagagattg
     2281 ccagacgcca atgcgcgcca gttgctgaca ggtacccgcc gccagcgcaa tcgctcgccc
     2341 atcaaagccc ggatgagcat gtgactctgg cgcggtcgct tcaatcaaat gtaccggcag
     2401 cgccccgtga cttaaccggg aaatagccag cgccagcgtc gcgcccgcca tgccgccacc
     2461 gacgatgatt acgctcattg ctttctcgca gctgccatca acgcttcgat ttcttccggc
     2521 tttttcacca cgctggcggt gaggttttcg ttaccggttt cggtaatcac aatgtcgtct
     2581 tcaatacgaa tgccgatacc gcgatattgt tctggcactt ctgcatccgg cgcaatatac
     2641 agccctggct ctacggtcag taccatgccc ggttccagaa tgcgcgagcg atcctggcca
     2701 taaacgccaa cgtcatggac atccagtcct aaccagtggc taaggccatg cataaagaaa
     2761 ggacgatggg cgttctgagc gatcagttca tcaacatcac ctttcaggat gccgagtttt
     2821 accaggccgc taaccatgat gcgcaccact tcaccggtga cttcctgaat ggaagttccc
     2881 ggacgataca ggcgcaggct ggtttcgaga gactcaagca caatgtcgta gatttcacgc
     2941 tgagcctggg tgaatttgcc gttgaccggg aaggtgcggg taatatcgcc agcgtaacct
     3001 ttgtattcac aacccgcatc aatcaacacc agatctccgt cacgcagttc acactcgttt
     3061 tcggtgtaat gcagaatgca gccgttttca ccgctgccga caatggtgtt ataggaagga
     3121 tagcgcgcgc cgtggcggtt aaattcgtgg tgaatttcgc cttccaggtg gtactcgaac
     3181 attcccggac ggcatttttc catcgcccgg gtatgcgcca gggcggtgat ttcacccgcg
     3241 cggcgaagta cggcaatctc ttccggcgat ttgaacaggc gcatttcatg aacaatggga
     3301 cgccagtcga tcatcgttgc cggcgcggtg agattttgcc gcgaaccttt acgcagtttc
     3361 tcaagcgcgc tgttcacgat ttcatcagca tatgcatatt cgccctgggc gtggtaaacc
     3421 acatccagac cattaagtag ttgataaagt tgctgattaa tttcgctgaa tgccagtgcg
     3481 cggtcaacgc ccagtttctc tggcgcggca tcctgaccta aacgacggcc aaaccagatc
     3541 tccgccgtca ggtcgcgaac gcggttaaac agaacgctgt ggttatgagt gtcatcgctt
     3601 ttaatcagca ccagcaccgc ttccggttcg ttaaagccgg tgaagtacca gaagtcactg
     3661 ttctgacgat aggggtattc gctgtcggcg ctacgtgtta cttctggtgc agcaaaaatc
     3721 agcgcggcgc tgccgggttg catttgctcc accagggcct gacggcgacg ctgaaactct
     3781 tgccgggata tctcactcat aacactctcc ttacgttttt tgtttttagt gtagagtcgg
     3841 tttttgtact tctggcgcgg tcggttgcgg atgagtaaag gtgtcgtggc ataacagcgc
     3901 ggcaacacga acgtattcga tgatctcttc aagcgacatt tcaagctctt cctgatcttc
     3961 gtcttcgtcg tagcccaact gcgcgatgtt acgcagatcg tcgatggctt caccggtttc
     4021 gccggtcact ttgtccagct tcggttgcgt aacgccaaga ccaagcagga agtgattgac
     4081 ccaaccggcc agcgcatcag cccgatcgaa aacgctgaca tcatcgccat caggcagata
     4141 aagctgaaaa aggaagccgt catcctgcag ggcatcgctg gtggcagagt gcattttacg
     4201 cagtgcctgt gccagctcat gaccgaaagc catgccttcg ttcgtcaggt cgtgaagtag
     4261 cggcagccat gagctgtcat cgttaccgcc acatatcatc ccactgatta aaccatgcat
     4321 ctcagcaggg gtcagacccg tcccttgttg gttcagatac tggttcattt cgttgtaacc
     4381 aggcatttcg ttctgtatag acataagcat tcgtcgtcaa agggaggaat attcatgata
     4441 tgctaccact ttgggccctg gtggaccaga aaagggcttg tctcttctca tcagggtagc
     4501 tatagtgtcg ccccttcgca gaccatgggt ctaaagacga aggcagcgca gtcaatcagc
     4561 aggaaggtgg catgtctgca caacccgtcg atatccaaat ttttggccgt tcactgcgtg
     4621 tgaactgccc gcctgaccaa agggatgcgt tgaatcaggc agcggacgat ctgaaccaac
     4681 ggttgcaaga tctgaaagaa cgcactagag tcacaaatac tgaacagttg gtcttcattg
     4741 ccgcattgaa tatcagctat gagttagcgc aagaaaaagc aaagactcgt gactacgcgg
     4801 caagtatgga acagcgtatt cggatgctgc agcagaccat agaacaagcg ttacttgaac
     4861 aaggtcgcat caccgaaaaa actaaccaaa actttgaatg acacttttcg gtttactgtg
     4921 gtagagtaac cgtgaagaca aaatttctct gagatgttcg caagcgggcc agtcccctga
     4981 gccgatattt cataccacaa gaatgtggcg ctccgcggtt ggtgagcatg ctcggtccgt
     5041 ccgagaagcc ttaaaactgc gacgacacat tcaccttgaa ccaagggttc aagggttaca
     5101 gcctgcggcg gcatctcgga gattcccttc ttatctggca ccagccatga cgcaactacc
     5161 agaactccca ctgacattat cccgacaaga aatccgcaaa atgattcggc aacgtcgtcg
     5221 tacgttaacg ccggaacaac agcaggaaat gggtcaacaa gccgctaccc ggatgatgac
     5281 ttatcccccg gtggtgatgg cacatacggt cgctgtattc ctctcttttg atggcgaact
     5341 cgacacccag ccgcttatag aacaactctg gcgcgccggt aagcgcgtat atcttccagt
     5401 tttgcatccc tttagtgccg gtaatttgct gttcctgaat taccatccgc aaagtgaact
     5461 ggtgatgaac aggttgaaga tccatgagcc aaaactggat gtgcgtgacg tgctacccct
     5521 ttcccgatta gacgtgttga tcacaccgct ggtcgccttt gatgagtacg gtcagcgcct
     5581 gggaatgggc ggtggttttt atgatcggac cttacaaaac tggcagcact ataaaatgca
     5641 accggtgggt tatgcgcatg attgtcagtt ggtggaaaaa ctccccgttg aagagtggga
     5701 tatccctctt cctgcggtgg ttacaccgtc gaaagtctgg gagtggtgag ggcgacacac
     5761 ctgcatcgcc ctgattgaca tcgttgattc ttt
//
LOCUS       DAZKFU020000274         5788 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZKFU020000274 DAZKFU020000000
VERSION     DAZKFU020000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.276"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..346
                     /locus_tag="ACHOQQ_RS21075"
                     /old_locus_tag="pgaptmp_004215"
     CDS             <1..346
                     /locus_tag="ACHOQQ_RS21075"
                     /old_locus_tag="pgaptmp_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4472400.1"
                     /translation="SRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGN
                     GGRERIGETGTVLGLNARDSKHTGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGL
                     MPLIRRRTMLQL"
     gene            536..649
                     /gene="yhiY"
                     /locus_tag="ACHOQQ_RS21080"
                     /old_locus_tag="pgaptmp_004216"
     CDS             536..649
                     /gene="yhiY"
                     /locus_tag="ACHOQQ_RS21080"
                     /old_locus_tag="pgaptmp_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGU4472401.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            712..1779
                     /gene="yhiI"
                     /locus_tag="ACHOQQ_RS21085"
                     /old_locus_tag="pgaptmp_004217"
     CDS             712..1779
                     /gene="yhiI"
                     /locus_tag="ACHOQQ_RS21085"
                     /old_locus_tag="pgaptmp_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4472402.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1776..4511
                     /gene="rbbA"
                     /locus_tag="ACHOQQ_RS21090"
                     /old_locus_tag="pgaptmp_004218"
     CDS             1776..4511
                     /gene="rbbA"
                     /locus_tag="ACHOQQ_RS21090"
                     /old_locus_tag="pgaptmp_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGU4472403.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDATHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4511..5635
                     /gene="yhhJ"
                     /locus_tag="ACHOQQ_RS21095"
                     /old_locus_tag="pgaptmp_004219"
     CDS             4511..5635
                     /gene="yhhJ"
                     /locus_tag="ACHOQQ_RS21095"
                     /old_locus_tag="pgaptmp_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4472404.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1283 a         1492 c         1665 g         1348 t
ORIGIN      
        1 tagccgggct atctcttgtc gatcctttaa tcgttatttt tctgtttcga cagattacag
       61 acctaaactt gaagcactat tagcatgctc acaaatgtta ggtataaatc aagttgttgc
      121 acataacggt aatggtggaa gagaacgcat aggtgaaacg gggacagttt taggtcttaa
      181 cgcacgtgac agtaaacata cgggaagaat gttcagcatg cataattgcc aaattaaccc
      241 tggtgcgggg cctgagataa ccactccctg gaagtcttac caacatgaaa aaaacagaaa
      301 tggacttatg cctttgatta gaaggcgcac aatgttacag ctctgaacca tagacaacat
      361 gaatattaat gttactatct ggttgagact gttgataaaa cgtaataagg aatgctctgt
      421 tcctgcaact gaacaggcgt gaaacgctat ccaacaggat ggataccgtt ttgtcagcca
      481 gttttataga tcacacttat gagtatgcgt gtttcattta cggccaggaa tgctgatgat
      541 gacaacatta ctacccgttt tcactaagcc ttccccgttg gcgctcaatg ctctgcgcgc
      601 tggtcgtatt tgtcgtttcc ttcttatccc ggatggaaga atccgctgaa cgtcgttcgg
      661 cggcagttgt aacacgcgtc gtttgcttac agggattatg gcgggtcgat tatggataag
      721 agtaagcgcc atctggcgtg gtggggtgtc gggttactgg cggtggctgc tatcgtggcg
      781 tggtggctgt tgcgcccggc aggtgtgccg gaaggctttg ccgtcagcaa tgggcgcatt
      841 gaagcgacgg aagtggatat cgccagcaaa attgccgggc gtatcgacac cattctggtg
      901 aaagaaggcc agtttgttcg cgaaggcgaa gtgctggcga agatggatac tcgcgtgttg
      961 caggagcagc gactggaagc catcgcgcaa atcaaagagg cacaaagcgc cgttgccgcc
     1021 gcgcaggctt tgctggagca acgacaaagc gaaactcgcg ccgcacagtc gctggttaat
     1081 caacgccagg ccgaactgga ctccgttgcc aaacgtcata cgcgttcccg ctcgctcgcc
     1141 caacgagggg ctatttctgc gcaacagctg gatgacgatc gcgccgccgc tgagagcgcc
     1201 cgagctgcgc tggaatcagc aaaagcccag gtttcggctt ctaaagccgc tatagaagcg
     1261 gcacgcacca atatcattca ggcgcaaacc cgcgtcgaag ccgcacaagc cactgaacgg
     1321 cgcattgccg cagatatcga tgacagcgaa ctgaaagccc cgcgtgacgg acgcgtgcag
     1381 tatcgggttg ccgagccagg ggaagtgctg gcggcaggcg gtcgggtgct gaatatggtc
     1441 gatctcagcg acgtctatat gaccttcttc ctgccaaccg aacaggcggg cacgctgaaa
     1501 cttggcggtg aagcccggct gatcctcgat gccgcgccag atctgcgtat tcctgcaacc
     1561 atcagttttg tcgccagtgt cgcccagttc acgccaaaaa ccgtcgaaac cagcgatgaa
     1621 cggctgaaac tgatgttccg cgtcaaagcg cgtatcccac cggaattact ccagcagcat
     1681 ctggaatatg tcaaaacggg cttgccgggc gtagcgtggg tgcgggtgaa tgaagaactt
     1741 ccgtggcctg acgacctcgt ggtgaggttg ccgcaatgac gcatctggaa ctggttcccg
     1801 tcccgcctgt cgcgcaactg gcgggcgtga gccagcatta tggaaaaacc gttgcgctga
     1861 acaatatcac actcgatatt ccggcccgct gtatggtcgg gctgattggt ccggatggcg
     1921 tcgggaagtc gagtttattg tcattgattt ccggtgctcg tgtcattgag cagggcaatg
     1981 tgatggtgct gggcggcgat atgcgcgacc cgaagcatcg ccgtgacgtt tgcccgcgca
     2041 tcgcctggat gccgcagggg ctgggcaaaa atctctacca caccttatcg gtgtatgaaa
     2101 acgtcgattt tttcgctcgc ctgttcggtc acgacaaagc ggagcgggaa gtccgaatta
     2161 atgagctgct gaccagcacc gggttagcac cgtttcgcga tcgtccggca gggaaactct
     2221 ccggcgggat gaagcaaaaa ctggggctgt gctgtgcgtt aatccacgac ccggaactgt
     2281 tgattcttga tgagccaaca acgggggttg acccgctctc ccgcgcccag ttctgggatc
     2341 tgatcgacag tattcgccag cggcagagca atatgagcgt gctggtcgcc accgcctaca
     2401 tggaagaggc cgaacgcttc gactggctgg tagcgatgaa tgccggagaa gtgctggcaa
     2461 ccggcagcgc cgaagagctg cggcagcaaa cgcaaagtgc cacgctggaa gaggcgttta
     2521 taaatctgtt accgcaagcg caacgccagg cgcatcaggc ggtagtgatc ccaccatatc
     2581 aacctgaaaa cgcagagatt gccatcgaag cgcgcgatct gaccatgcgt tttggttcct
     2641 tcgttgccgt tgatcacgtt aatttccgca ttccacgcgg ggagattttt ggttttcttg
     2701 gttcgaacgg ctgcggtaaa tccaccacca tgaaaatgct caccggactg ctgcccgcca
     2761 gcgaaggtga ggcgtggctg tttgggcaac cggttgatcc aaaagatatc gatacccgcc
     2821 gtcgggtggg ctatatgtcg caggcgtttt cgctctataa cgaactcacc gtgcggcaaa
     2881 acctggagtt acatgcccgt ttgtttcaca tcccggaagc ggaaattccc gcgcgagtgg
     2941 ctgaaatgag cgagcgtttt aagctcaacg acgttgaaga tgttctgccg gagtcattgc
     3001 cgctcggcat tcgccagcgg ctttcgctgg cggtggcggt gattcatcgc ccggaaatgt
     3061 taatcctcga tgagcctact tctggcgtcg atccggtggc gagggatatg ttctggcagt
     3121 tgatggtcga tctctcgcgc caggacaaag tgaccatttt catctccacc cactttatga
     3181 acgaagcgga acgttgcgac cgtatctcac tgatgcacgc cggaaaagtg ctcgccagcg
     3241 gtacaccgca ggaactggtt gagaaacgcg gagccgccag tctggaagag gcatttatcg
     3301 cctatttgca ggaagcggca ggacagagca acgaagccga agcgcctccc gtgatacacg
     3361 atgctacaca cgcgccgcgc cagggattta gcctgcgccg tctgtttagc tacagccggc
     3421 gcgaagcact ggaattgcgc cgcgatccgg tccgttcgac gctggcgctg atgggaacgg
     3481 tgatcctgat gctgataatg ggttacggca tcagtatgga tgtggaaaac ctgcgctttg
     3541 cggtgctcga ccgcgaccag accgtcagta gtcaggcgtg gacgctcaac ctctccggtt
     3601 cccgttactt tatcgaacag ccgccgctta ccagttatga cgagcttgat cgtcggatgc
     3661 gtgcgggcga tatcacggtg gcaattgaga tccctccaaa tttcgggcgt gatatcgcgc
     3721 gtggtacgcc tgtggaactc ggcgtctgga tcgacggagc gatgcccagt cgtgccgaaa
     3781 cggtaaaagg ttacgtgcag gctatgcacc agagctggct acaggatgtg gcgagccgac
     3841 aatccacacc ctccagccaa agcgggctga tgaacattga gacgcgctat cgctataacc
     3901 cggacgtaaa aagcctgcca gcgattgttc cggcggtgat cccgcttctg ctgatgatga
     3961 tcccgtcaat gctaagcgcc cttagtgtgg tgcgggaaaa agaactaggg tcgattatca
     4021 acctttacgt gactcccacc acccgtagcg aatttttgct tggcaaacag ctaccgtaca
     4081 tcgcgctggg gatgctgaac tttttcctgc tctgcggtct gtcggtgttt gtgtttggcg
     4141 taccgcataa aggcagtttc ctgacgctca ccctggcggc gctgctgtat atcatcatcg
     4201 ccaccggaat ggggctgctg atctccacct ttatgaaaag ccagattgcc gccattttcg
     4261 gtacggcgat tatcacgtta atcccggcga cacagttttc cgggatgatc gatccggtag
     4321 cttcgctgga aggtccagga cgttggatcg gcgaggttta cccgaccagt cattttctga
     4381 ctatcgcccg cggaacgttc tcgaaagcgt tggatctgac tgatttatgg caacttttta
     4441 tcccgttgct gatagccata ccgctggtga tgggcttaag catcctgctg ctgaaaaaac
     4501 aggagggatg atgcgccatt tacgcaatat ttttaatctg ggtatcaaag agttgcgcag
     4561 tctactcggt gataaagcga tgctgacatt gattgtcttc tcgtttacgg tgtcggtgta
     4621 ttcgtcagcg accgttacgc caggatcgtt gaacctggca ccgatcgcca ttgccgatat
     4681 ggatcaatcg cagttatcga atcggatcgt taacagcttc tatcgcccgt ggtttttgcc
     4741 accggagatg atcaccgccg atgagatgga tgccggactg gacgccggac gctatacctt
     4801 cgcgataaat attccgccta attttcagcg tgatgtcctc gctggacgcc agccagatat
     4861 tcaggtgaac gtcgatgcca cgcgcatgag ccaggcattt accggcaacg ggtatatcca
     4921 gaatattatc aacggtgaag tgaacagctt tgtcgcgcgc taccgtgata atagcgagcc
     4981 gttggtatcg ctggaaaccc ggatgcgctt taacccgaac ctcgatcccg cgtggtttgg
     5041 cggggtgatg gcgatcatca acaacattac tatgctggcg attgtattga ccggatcggc
     5101 gctgatccgc gagcgtgaac acggcacggt ggaacactta ctggtgatgc cgataacgcc
     5161 gtttgagatc atgatggcga aggtctggtc gatggggcta gtggtgctgg tggtatcggg
     5221 attatcgctg gtgctgatgg tgaaaggcgt gctgggcgta ccgattgaag gctcgatccc
     5281 gctgtttatg ctgggcgtgg cgctcagtct gtttgccacc acgtcaatcg gcatttttat
     5341 ggggacgata gcgcgttcaa tgccgcaact ggggctgctg gtgattctgg tgctgctgcc
     5401 gctgcaaatg ctttccggtg gatccacgcc gcgcgaaagt atgccgcaga tggtgcagga
     5461 cattatgctg accatgccga cgacacactt tgttagcctc gcgcaggcca ttctctaccg
     5521 gggtgccgga ttcgaaatcg tctggccgca gttcctgacg ctgatggcaa ttggcggcgc
     5581 gtttttcacc attgcgctgc tgcgattcag gaagacgatt gggacaatgg cgtaaataca
     5641 attgcccgat gcgtaagctt atagggacta cgcatcggtg caatttattg aattttagtt
     5701 gtttttaggc cggataaggc gtttatgaaa tgacccccaa aacctggaca gtttcagtta
     5761 accggctttt tgatactcag tccggtat
//
LOCUS       DAZKFU020000275         5739 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZKFU020000275 DAZKFU020000000
VERSION     DAZKFU020000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.277"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(133..3657)
                     /gene="nifJ"
                     /locus_tag="ACHOQQ_RS21100"
                     /old_locus_tag="pgaptmp_004220"
     CDS             complement(133..3657)
                     /gene="nifJ"
                     /locus_tag="ACHOQQ_RS21100"
                     /old_locus_tag="pgaptmp_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGU4472405.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKVRF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            3931..4197
                     /gene="ydbJ"
                     /locus_tag="ACHOQQ_RS21105"
                     /old_locus_tag="pgaptmp_004221"
     CDS             3931..4197
                     /gene="ydbJ"
                     /locus_tag="ACHOQQ_RS21105"
                     /old_locus_tag="pgaptmp_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGU4472406.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(4194..4616)
                     /gene="hslJ"
                     /locus_tag="ACHOQQ_RS21110"
                     /old_locus_tag="pgaptmp_004222"
     CDS             complement(4194..4616)
                     /gene="hslJ"
                     /locus_tag="ACHOQQ_RS21110"
                     /old_locus_tag="pgaptmp_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGU4472407.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4727..5716)
                     /gene="ldhA"
                     /locus_tag="ACHOQQ_RS21115"
                     /old_locus_tag="pgaptmp_004223"
     CDS             complement(4727..5716)
                     /gene="ldhA"
                     /locus_tag="ACHOQQ_RS21115"
                     /old_locus_tag="pgaptmp_004223"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4472408.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
BASE COUNT         1339 a         1488 c         1481 g         1431 t
ORIGIN      
        1 atgcctttat tgtcacagat tttattttct gttgggccat tgcattgcca ctgattttcc
       61 aacatataaa aagacaagcc cgaacagtcg tccgggcttt ttttagaatt ggataatcct
      121 tatccagagc atttaatcgg tgttgctttt ttccgctttt ccggccattt gtgccaggaa
      181 gtcatagcgt ttttgcaaat cagctgcagc atctttccat aactgttctg ccacttctgg
      241 ctgctgcgaa ttcagacgac ggaaacgttg ctcgtgaagt aacgtttctt ccagtgcttc
      301 tgacggcggg cgtgaatcca gcgccagcgg cagtttgcct tcatcggcac gacgcggatc
      361 gaagcgatat agcggccaga agccggtagc tgtgagttgg cgcatctggt catggctgag
      421 tgccagatcg taaccatgct cttcacatgg gctataagcg atgatcagcg atggtcccgg
      481 atacgcttcc gcttcctgaa tcgctttcac cgtctggttc agttgcgcgc cgagagaaat
      541 ctgcgccaca taaacatgac cgtacatcat catactgacg ccaagatctt tacgcgcttt
      601 acgtttgccg tgctcgccga acttagttac tgcacccagc ggtgtcgctt tcgacgcctg
      661 accaccggtg ttggaatagc attgcgtatc cagcaccaga atgttgacgt tttccgtcaa
      721 actcaataca tgatccagac cgccaaagcc gatatcgtaa gcccagccgt cgccaccaat
      781 cagccagatt gatttttcta ccagtgcatc tgcgtcacgc agcagttcat gtgcttcggc
      841 aacatcgttg agttgctggc gtaaagctgc gacctgttct cgacgaacct ctggcgtagc
      901 gtctgatttc aacgccgtca gtaattccgc cgggatttta tcggcaaatt gatccagcag
      961 acgcagcacg cggacacggt gttgatcgac cgtcaggcgg aaaccaaggc caaattcggc
     1021 attatcttca aatagagagt tcgcccatgc cggcccacga ccgttggcat cggtggtata
     1081 cggtgtagag ggcaggttac cgccataaat tgaagaacag ccggtggcgt tagcgatcaa
     1141 catccggtcg ccatagagct gagtcagcaa tttaatatac ggcgtctcgc cacaaccgga
     1201 gcaagcaccg gaatattcaa acagcggtgt aatcagctgc gatgtacgaa tatcaatacg
     1261 ttccagtttg ctacggtcga tttctggcag gttgaggaag aaatcgtaat tgattttctc
     1321 ttcttcgaca tgttcaaggc gcgacatcat attgatggct ttaatctctg gattctgacg
     1381 gtctttcgcc gggcaaactt cgacgcacag gttacaaccg gtgcaatctt ccggagccac
     1441 ctgcaagaca tatttctgcc cgcgcatatc acgcgatttc acatccagcg aatgcaggct
     1501 ggcaggggcg ttttccatcg cttcaggcgg taccactttt gcgcgaatag ctgagtgtgg
     1561 gcaagcggca acacagtggt tacattgggt acagagttcc tctttccaga tggggatctc
     1621 ttcggcgata ttgcgttttt cccagcgtgt agtgcccatc ggccaggtgc cgtctggcgg
     1681 cagcgccgaa acggggaggg catcaccaag cccggcgagc atcgccgcgg ttacggtttt
     1741 cacgaaatca ggggcggcat cggaaaccac tggcggtcga ttggcgctgt gcggattaac
     1801 cggctgcaac ggaacttctt ctacggattc acgcgccagc gccagagcct gccagttgcg
     1861 ttccaccaga tcctgacctt tgctactgta acttttggca atcgcaccct gcaattctgc
     1921 gagggcgcta tcgccaggca gaatttgcgt cagatggaaa aaagccatct gcatgacggt
     1981 attaatacgg gccgccaggc cacattcgcg ggcgattttc gccgcgttaa tcacatagaa
     2041 gcgcgctttt ttctgattta gcacggcctg aacttcctgc ggcaagcgcg accacacttc
     2101 atctgcgctg tacggcgtgt tgagcaggaa aatgccgcca ggttttaaac gctcagccat
     2161 ctgatattta tcgataaact gcaactggtg gcagccaaca aaatcagcct gggaaatgag
     2221 ataagcggaa cgaatcggct gttcgctcac tcgaaggtga gaaaccgtca ggccgcccgc
     2281 ctttttggag tcgtaaacaa aatagccctg tgcgtaccac ggcgtggaat taccgataat
     2341 cttgatattg tttttggtcg cggaaacgct gccatcacta ccaagcccat aaaacaaggc
     2401 ttccagtttc gccgagtttg gcagggtgtt ttccggcaac ggcagtgaca gattggtcac
     2461 atcatcgtaa ataccaaccg taaagcgcac tttcggttta gccgcgttga gctcggcaaa
     2521 taccgccagt acacagtccg ggccaaattc tttggatgaa agaccatagc gcccaccaat
     2581 gacacggggc agagtttcgc gctcgccatt attaaaggct tctgccagtg cggtcattac
     2641 atccagatag agcggttctg cctgggcacc gggttctttg gttctgtcca gtaccgccac
     2701 gctgcgtacg gatcccggca gagcttgcag caagtgtttg gcggagaagg gacggtacag
     2761 acgaactttc agcacgccaa ctttttcgcc acgggttagc aattcatcaa ccacttcttc
     2821 acaggtacca atggcagagc ccatcaggat aatcacccgt tccgcttgcg gatgcccgta
     2881 atattcaaac ggctgatact gacgacctgt cgcggcagcg aaatcattca tcgcctgttc
     2941 aacatggtca tagaccgcgt tgtaccatgg gttggtggct tcgcgagact ggaaataagt
     3001 gtcaggattg gcggacgtac cgcggatcac cggatgttcc gggttgagtg cccgggcgcg
     3061 atgagcatca atttcggcct gcggcatgag atcaagaatc gtgtcatcgg ccagcgggac
     3121 aattttattg atttcgtggg acgtgcggaa accatcaaag aaatgaataa atggcacgcg
     3181 gcttttcagc gtcgcgatgt gcgaaatgag agcaaagtct tgcgcttcct ggacgtttgc
     3241 cgcacacaac atcgcgcaac ccgtctggcg caccgccatg acgtcggaat gatcgccaaa
     3301 aatagagagt gcatgtgtgg caacggtacg tgccgctaca tgcaggacaa acggtgtgag
     3361 ttcgcctgcc agtttgtaca gcgtcgggat catcagcagc aaaccctgcg atgacgtaaa
     3421 cgatgttgaa agggcacccg tctgcaaagc gccatgcacg gtagcgatag cacccgcttc
     3481 cgactgcatt tcaaccacgc gtggtgtgtc tccccaaacg ttcttcaagc cgtttccggc
     3541 ccaggcatca gcctgttctg ccatcgtgga actgggggta atagggtaga tggcgataac
     3601 ttcactggtg cgaaacgcca ccgaagcaac cgcgccatta ccgtcaatag taatcatatg
     3661 acacccttac attgcgcaaa tgaggggcgc acgaaattgc tgcgcgccca gtagtaatct
     3721 ttcaatttta gcaaatggct ttcttctgca ttttcgcttt tgtgtccccc acatcagcgt
     3781 aatgaatgtt ttgatcaaac agagggcaaa aaaatagcca taaaaaagta aaaatgcggg
     3841 gcaacgcgca ggacagctct cgacgtcgcc ctctgtgctg cctattattt atggcggtgt
     3901 cgtttggctt gaagatcaga ggaagaagag atgcgagcag cgttttgggt agggtgtgcc
     3961 gctttattgt tgtcggcgtg tagtagtgag cctgttcagc aggcgactgc ggcgcacgta
     4021 gcgccaggtt taaaagcgtc gatgtccagt agtggagaag caaattgtgc aatgatcggc
     4081 ggttcgcttt ctgttgcccg tcaactggat ggtacggcga ttgggatgtg tgcattaccc
     4141 aacggcaaac gctgtagcga acagtcactt gccgccggga gctgtggcag ctattaattc
     4201 attaaatccg ccagcttata agttaatgtc tgtttcgcgg tcgccagcgt taactggttc
     4261 gcggtcagat ccacttgtgc accttctttc agcatttcgc taatggtgtt atcgagttca
     4321 ttaagctgcg ggttagcgca catcatacgg gtcattgcca gccctttggc tgtcagttca
     4381 ccattagaca gtttgccttc accgctaaag cggttacaca tgctgccgga aatcatcatt
     4441 ttttcaccaa agctgatttc tggcggattt ttatcgctgg tcacgggctt accgtttacg
     4501 ctttccagca caaagcgatg atgctgtagc tgttctggcg taacagcaat tttgtcatta
     4561 cttacacatc ccgccatcag caggcttagc gcaacaagcg cggctacttt cttcattgtg
     4621 gttctcaatt acagtttctg actcagggct attttaagaa tagaggatga aaggtctttg
     4681 gggattatct gaataagctc ccctggaatg caggggagcg gcaagattaa accagttcgt
     4741 tcgggcaggt ttcgcctttt tccagattgc ttaagttttg cagcgtagtc tgagaaatac
     4801 tggtcagagc ttctgctgtc aggaatgcct ggtgcccggt aaacagcacg ttgtggcagg
     4861 cagacaggcg acggaatacg tcatcctgga tcacgtcgtt ggatttatct tcaaagaaca
     4921 gatcgcgttc gttctcatac acgtccatac ccaacgaacc aattttctga tttttcagcg
     4981 cttcaattgc tgcctgtgaa tcaatcaatg caccgcgact ggtattgacg atcatcacgc
     5041 catttttcat ctgatcgaag gcggcttcgt tcaacagatg gtagttttcc ggtgtcagcg
     5101 ggcagtgcag agagataacg tctgattcag agaacagggt tggcagatcg acatactcca
     5161 caccgagttc cagcgccgct gcacttggat acggatcgaa cgccagcaga cgcataccaa
     5221 aacctttcag aatgcgcagc atcgccacac cgattttacc ggtaccgata acgcctgccg
     5281 ttttgccata catagtaaag ccggtcagac cttccagaga gaagttagcg tcacgggtac
     5341 gctgatacgc gcggtgaata cggcggttca gcgtcatcat cataccgatg gcgtgttcag
     5401 caacggcctc tggatcatag gctggaacac ggactacttt cagccccagt tcttttgccg
     5461 cgtcaaggtc gacgttattg aaaccggcac agcgcagggc gatatattta acgccgtgct
     5521 ttttcagctc ttccagcacc gggcggctgc cgtcatcgtt tacgaaaata cataccgctt
     5581 cgcagccatt ggcagtttta gcggtttttt ccgtcagcag aaagtcaaaa aattccagct
     5641 caaagccaaa ggactcgttc acctgttgca ggtacttctt gtcgtactgt tttgtgctat
     5701 aaacggcgag tttcataaga ctttctccag tgatgttga
//
LOCUS       DAZKFU020000276         5735 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZKFU020000276 DAZKFU020000000
VERSION     DAZKFU020000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.278"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..1692
                     /gene="recN"
                     /locus_tag="ACHOQQ_RS21120"
                     /old_locus_tag="pgaptmp_004224"
     CDS             31..1692
                     /gene="recN"
                     /locus_tag="ACHOQQ_RS21120"
                     /old_locus_tag="pgaptmp_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGU4472409.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            1841..2182
                     /gene="bamE"
                     /locus_tag="ACHOQQ_RS21125"
                     /old_locus_tag="pgaptmp_004225"
     CDS             1841..2182
                     /gene="bamE"
                     /locus_tag="ACHOQQ_RS21125"
                     /old_locus_tag="pgaptmp_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU4472410.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(2244..2534)
                     /gene="yfjF"
                     /locus_tag="ACHOQQ_RS21130"
                     /old_locus_tag="pgaptmp_004226"
     CDS             complement(2244..2534)
                     /gene="yfjF"
                     /locus_tag="ACHOQQ_RS21130"
                     /old_locus_tag="pgaptmp_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGU4472411.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(2524..2961)
                     /gene="ratA"
                     /locus_tag="ACHOQQ_RS21135"
                     /old_locus_tag="pgaptmp_004227"
     CDS             complement(2524..2961)
                     /gene="ratA"
                     /locus_tag="ACHOQQ_RS21135"
                     /old_locus_tag="pgaptmp_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGU4472412.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            3132..3614
                     /gene="smpB"
                     /locus_tag="ACHOQQ_RS21140"
                     /old_locus_tag="pgaptmp_004228"
     CDS             3132..3614
                     /gene="smpB"
                     /locus_tag="ACHOQQ_RS21140"
                     /old_locus_tag="pgaptmp_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGU4472413.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            3829..4191
                     /gene="ssrA"
                     /locus_tag="ACHOQQ_RS21145"
                     /old_locus_tag="pgaptmp_004229"
     tmRNA           3829..4191
                     /gene="ssrA"
                     /locus_tag="ACHOQQ_RS21145"
                     /old_locus_tag="pgaptmp_004229"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            4375..5568
                     /locus_tag="ACHOQQ_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /pseudo
     CDS             4375..5568
                     /locus_tag="ACHOQQ_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101258.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
BASE COUNT         1576 a         1466 c         1362 g         1331 t
ORIGIN      
        1 agtaatggtt tttcatacag gaaaacgact atgttggcac aactgaccat cagcaacttt
       61 gctatcgttc gtgagcttga gattgatttt catagcggca tgaccgtaat aactggcgag
      121 accggcgcgg gtaaatctat tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct
      181 gaagccgaca tggtgcgtac cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg
      241 aaagatacgc cagcggccct gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa
      301 tgtttgcttc gtcgcgtgat cagcagcgat ggtcgctccc gtggtttcat caacggtaca
      361 gctgttcctc tgtcacaact gcgcgaactg ggtcagttgc tgattcagat ccatggtcag
      421 cacgctcatc agttactcac caaacccgag caccaaaagt tcctgcttga tggctatgcc
      481 aatgaaacct ctctactcca ggaaatgacc gcacgttatc agttgtggca tcaaagctgc
      541 cgtgacctcg cgcatcatca acagttaagt caggaacgcg ccgcccgtgc ggaactgctg
      601 caataccaat taaaagaact taacgaattt aatccgcagc ccggagagtt tgagcaaatc
      661 gacgaagagt acaaacgtct ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca
      721 ttggcattaa tggccgacgg tgaagacgca aacctgcaaa gtcagcttta cacggctaaa
      781 caactggtga gcgaattgat tggcatggac agcaaactgt ccggcgtact tgatatgctg
      841 gaagaagcta ccatccagat cgctgaagcc agcgacgaac tgcgccacta ctgcgatcgt
      901 ctggatctcg atcccaaccg actgtttgaa cttgaacagc gcatctcaaa acagatttcg
      961 ctggcacgta aacatcacgt cagccctgag gcattaccac agtattacca gtcgctactg
     1021 gaagaacagc agcaactgga cgatcaggcc gactcacaag aaacgctcgc gctggcggta
     1081 acgaaacatc atcagcaggc actggaaacg gcgcgcgcat tacaccagca acgccagcat
     1141 tatgcagaag aacttgcaca gctgatcacc gacagtatgc atgcgctctc aatgccgcat
     1201 gggcagttta cgattgatgt taaatttgac gagcatcacc tgggcgctga cggtgccgat
     1261 cgtattgagt ttcgggtaac caccaaccca ggtcagccaa tgcagcctat tgccaaagtc
     1321 gcatccggtg gtgaattgtc ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa
     1381 atggaaaccc cggcactgat ttttgatgaa gtggatgtag ggattagcgg tccaacagcg
     1441 gcagttgtcg gcaaactgct gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc
     1501 cacctgccac aagtcgcggg atgtggtcat caacactatt ttgtcagcaa agaaaccgat
     1561 ggtgcgatga cagaaacgca tatgcaatct ctggataaaa aagcgcggtt acaagagctg
     1621 gcgcgcctgc ttggtggcag tgaagtcaca cgtaacacac tggcgaatgc gaaagaactg
     1681 cttgcggcgt aaactttttt cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga
     1741 ccggaaagca aaaggtttta aagtgatgaa aggtctatta tcatcggcat attacagatg
     1801 agccacgtac tgctcgggcc cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac
     1861 tgctgcagca gcagtactat tgatgttgac cgcaggctgt tccactctgg agcgagtggt
     1921 ttaccgtcct gacatcaacc aggggaacta tctgaccgct aacgacgtat ccaaaatacg
     1981 tgttggcatg acgcaacaac aagttgcgta cgcattgggt acaccgctga tgtccgatcc
     2041 atttggtacg aatacctggt tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac
     2101 gcagcaaacg ctgacgctaa cctttaacag tagcggtgtg ttgaccaata ttgataacaa
     2161 acctgcgctg agtggtaact aataataaag ttgctctcaa agacgttaaa aaaggtgctc
     2221 aatgagcacc ttttttctgt ctgttattta ttcgctgatt tttctgctcg ttgcctgcga
     2281 agctctttcg gatcggcaat gagagggcga taaatctcca cccgatcgcc atcatgcaca
     2341 ctatcgctta gttttgccgg acggctgtaa atgccgactt tgtttttagt taaatcgata
     2401 tcggtacgca actccagcaa gccactggcg cgaatagctt cttcaaccgt cgcgccctcc
     2461 tgcagcgtca cgcgctgcag gtactgcttc tcaggtagcg cataagccac ctcaacggca
     2521 attttacctg gcactgtaaa cctctttcgc acgaaccgta aaagcctgga ccatattaga
     2581 cgccagctct ttaaacacgc gaccaaaagc gagttcaatc aacttattgg taaactcaaa
     2641 gtcgagatga aactcgatac gacacgcctc ctggctcagc ggcgtaaact tccatccacc
     2701 aatcaatttc ttgaacggcc catccaccag attcataaga atactttggt tactggtcaa
     2761 ctggttgcgg gtagtaaacg ttttgctgat cccagcctta gagacatcta ccgccgcagt
     2821 catctgccca ggagtggact ccagaatccg acttccggta caacccggca aaaactgagg
     2881 ataagactga acgtcattca ctaactgata catttgctcc gcgctgtagg gtaccagtgc
     2941 ggtccggcta atctgaggca taacaatttc catcaacaaa aatccaacaa ataatatcat
     3001 ttatcctgct gtaaaaaaaa cgctatcccg gcgctgggta acatcgggtt catgctaaga
     3061 tagagccttg tcccccgcag gattgatatg gggtgttttc gatttcagat taccgatgat
     3121 tcacgacgct tatgacgaag aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta
     3181 acaagcgcgc ccgtcacgaa tactttatcg aagaagagtt cgaagcggga cttgccctgc
     3241 aaggctggga ggttaaatcc ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc
     3301 ttctgcgtga cggagaggca tttctgtttg gcgctaacat cacgccaatg gccgtggcct
     3361 ccacgcatgt ggtgtgcgat cctacccgta cccgcaagtt acttctcaac cagcgcgaac
     3421 tggactcatt gtacggtcgc gtcaatcgag aaggctatac cgtagtggcg ctctccctgt
     3481 actggaaaaa tgcctggtgc aaagtgaaaa tcggcgtcgc caaaggtaag aaacagcacg
     3541 ataaacgttc agatatcaaa gaacgcgaat ggcaggtgga taaagcgcgt atcatgaaaa
     3601 acgctcaccg ttaaacctgc actccaatta ttgaccagtt cctcaccgcg cctccctctc
     3661 cggcggcgcg aatgaacatc ttattggcta tcacatccga cgaaaatgtt gccatcccat
     3721 tgctaaatcg aataaaaatc aggctacatg gtcgctaaat ctttaacgat aacgccattg
     3781 aggctggtca tggcgctcat aaatctggta tacttacctt tacacattgg ggctgattct
     3841 ggattcgacg ggatttgcga aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa
     3901 agccgcaaaa aatagtcgca aacgacgaaa actacgcttt agcagcttaa taacctgctt
     3961 agagccctct ctccctagcc tccgctctta ggacggggat caagagaggt caaacccaaa
     4021 agagatcgcg tggaggccct gcctggggtt gaagcgttaa aacttaatca ggctagtttg
     4081 ttagtggcgt gtccgtccgc agctggcaag cgaatgtaaa gactgactaa gcatgtagta
     4141 ccgaggacgt aggaatttcg gacgcgggtt caactcccgc cagctccacc aaatgatgat
     4201 ccggatacgt ccggtgaagt acagaaagcc cgcacggcac aagccctgcg ggcttttttg
     4261 tgtctgtcgt tgtccgagaa catccggcta aatccagaga aaattggtac acgtttaggt
     4321 acacgctata ctgtggtcca ttaaacgtgt accaattatg gaagggatcc agacatggcg
     4381 cgcattacac gccccctcac taacaacgaa atcctcaaag cgaaaccccg cgaaaaagac
     4441 ttcaccctgc atgatggtga tggcctgttc ttactcgtca aaacctccgg taaaaaactc
     4501 tgacgcttcc gttatcaacg accgggaagc agcagccgca caaatttgag tctcggctca
     4561 taccctgccc ttacactcgc agcagctcgt cagatacgcg accagcactt aaccacgctc
     4621 gcgcaaggca tggatccaca acagcaacag gagcaagcgt cagcacaacg ccagattgag
     4681 ttagacagca ttttctcaac tgtggccgct aactggttcc agattaaaag cagaagtgtc
     4741 acagaggatt atgcaaagga tatttggcgt tctttggata aagacgtgtt cccggcaatc
     4801 ggtgcgatgc ccgttcagga tataaaggcc agaacgattg ttgaagcatt ggagccaatc
     4861 aaagcacgtg gcgcattaga gacggttcgt cgcctagttc agcgtattaa cgaaataatg
     4921 atttatgcgg ttaataccgg tctgatagat gccaacccgg cttcgggtgt aggaatggct
     4981 tttgagaaac ccaaaaagca aaatatgccc acgctacggc cagaagaatt acctaagctt
     5041 atgcgttccc tggtcatgtc gaatctgtct gttccgactc gctgtcttat tgaatggcag
     5101 cttctgaccc tagtgcgccc ttctgaggct tctggtgctc ggtgggtaga gatcgatctc
     5161 aatgccaaac tctggacgat tcccgccgaa cgaatgaagg ctaagcgtga acacattgtt
     5221 cctttatcac cacaagcatt agaaattctg gatgtgatga aacctattag tgcacatcga
     5281 gaacatgttt ttcccagcag aaataatcca aaacaaccta tgaatagcca aactgccaat
     5341 gctgcactta agcgaatagg ttatggtggg aaattggttg cacatggttt acgttcgatt
     5401 gcaagtacgg caatgaatga agccggtttt aatgctgatg taattgaggc tgctcttgct
     5461 cattcagata gaaatgaagt acgaaaagcc tacaatcgct caacatattt acacgcaaga
     5521 attgaattga tgaattggtg gggacgacat attcatcccc accagtaaac tcactctgga
     5581 gttcctaagg cagtaataaa accagcgtca aggaactcca cattatcgga tagactatct
     5641 atcaatgctc ttctccaatg attaagccca taacgtctgg tatagtcatc cctagttctt
     5701 tttagaaata atcttaatcc ttcttggcct gcaga
//
LOCUS       DAZKFU020000277         5725 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZKFU020000277 DAZKFU020000000
VERSION     DAZKFU020000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.279"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..559)
                     /locus_tag="ACHOQQ_RS21155"
                     /old_locus_tag="pgaptmp_004231"
     CDS             complement(<1..559)
                     /locus_tag="ACHOQQ_RS21155"
                     /old_locus_tag="pgaptmp_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HGU4472414.1"
                     /translation="MHSENIAAYIGLDVHKETLAVAIAAPERLGEVRYYGTINNEAQA
                     VRRLFQKLQGLYGNILSCYEAGPCGFGLYHQLTAMNIKCQVIAPSRIPKSPTDRIKND
                     HRDAISLARLLRAGELTPVWIPDLTHEAMRDLIRARAAAKRDSRVARQRILSMLLRTD
                     KRYAGKHWTGKHRTWLANQSFSQPSQ"
     gene            620..808
                     /locus_tag="ACHOQQ_RS21160"
                     /old_locus_tag="pgaptmp_004232"
                     /pseudo
     CDS             620..808
                     /locus_tag="ACHOQQ_RS21160"
                     /old_locus_tag="pgaptmp_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1176..2432)
                     /locus_tag="ACHOQQ_RS21165"
                     /old_locus_tag="pgaptmp_004233"
     CDS             complement(1176..2432)
                     /locus_tag="ACHOQQ_RS21165"
                     /old_locus_tag="pgaptmp_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU4472415.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLSDFANIFQYAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     NIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(2445..2732)
                     /locus_tag="ACHOQQ_RS21170"
                     /old_locus_tag="pgaptmp_004234"
     CDS             complement(2445..2732)
                     /locus_tag="ACHOQQ_RS21170"
                     /old_locus_tag="pgaptmp_004234"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4472416.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            complement(2748..3191)
                     /locus_tag="ACHOQQ_RS21175"
                     /old_locus_tag="pgaptmp_004235"
     CDS             complement(2748..3191)
                     /locus_tag="ACHOQQ_RS21175"
                     /old_locus_tag="pgaptmp_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4472417.1"
                     /translation="MLKNLLNTKVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            3462..4493
                     /locus_tag="ACHOQQ_RS21180"
                     /old_locus_tag="pgaptmp_004236"
     CDS             3462..4493
                     /locus_tag="ACHOQQ_RS21180"
                     /old_locus_tag="pgaptmp_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4472418.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKTSSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPF
                     SPAQITIETAFFEASHKLTEHMLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSYYQ
                     NSDLVITMPGAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDSCDQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            4696..5376
                     /locus_tag="ACHOQQ_RS21185"
                     /old_locus_tag="pgaptmp_004237"
                     /pseudo
     CDS             4696..5376
                     /locus_tag="ACHOQQ_RS21185"
                     /old_locus_tag="pgaptmp_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5393..5701
                     /locus_tag="ACHOQQ_RS21190"
                     /old_locus_tag="pgaptmp_004238"
                     /pseudo
     CDS             5393..5701
                     /locus_tag="ACHOQQ_RS21190"
                     /old_locus_tag="pgaptmp_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         1619 a         1370 c         1314 g         1422 t
ORIGIN      
        1 gctgagatgg ctgactaaat gattgattag ctaaccaggt tcgatgtttg ccggtccagt
       61 gcttccctgc ataacgtttg tctgttcgca atagcatgct cagaattcgc tgtcgggcaa
      121 ctctgctgtc tcgtttagct gctgcacggg ctctgattag atcccgcata gcttcatgag
      181 tcaggtcagg gatccagaca ggggtaagtt caccagcccg cagtaacctg gcaagagaaa
      241 ttgcgtcccg atgatcattt ttgattctgt ctgtcggtga ttttggtatc cgggaagggg
      301 caataacctg gcattttata ttcatcgctg tgagttgatg atatagccca aaaccacagg
      361 ggccagcctc atagcaggaa agaatgtttc cgtaaagtcc ctgcaatttc tgaaataatc
      421 gacgtacagc ctgggcttcg ttgttaatcg taccataata acgaacttcg ccaagccgtt
      481 caggtgcagc aatagcaacc gccaacgttt ctttatgtac atccagcccg atgtaagctg
      541 caatattttc cgaatgcata atgacctcct ctggaatatc ccaccaatgc tatagtgagt
      601 atataaaggt cataccatct ttatgttctt tggcagcggt cacggcggcg agcgtggagc
      661 actgttgtac gggctgatcg gcacctgccg tctgaacggt atcgatccgg aagcgtatct
      721 gcgccatatc ctgagcgtac tgccggaatg gccttccaac cgagttgatg aactcttgcc
      781 atggaacgta gtactcacca ataaataagc gtcaatacgg tgctccgttg acgcttacga
      841 tcaaagggcg tatatcacgg cgtcttccgg taaggaactc ttctggcatt ggcgttgaat
      901 tattacctgt ggagatgacg ctggatgagc ggccttaccc cgctttttac gcgaggtgat
      961 tatgaagatc aatgactagc cgtcgaaccg tgtgcatgaa ctgctgcact gaaacttctc
     1021 agctgtaaaa taatccttac gctacgtact tcccggagcg cttacgatta atcggatgca
     1081 gtcctgactg gaagaaagaa aaatgggaaa aaataagagg aattaatatc ctccacgttc
     1141 ctgaaatcct ggaggatatt aatgtcatca aaatattagc gaacgataat ccctaacaaa
     1201 atacccagtg cgccaaagtc agcatcactg aaggtggtgt tagcaaaacc aatgttaccc
     1261 agcacaggca ataagaatac tggcaggaag gtaataagca agccctgagc gaaggcaccc
     1321 aaaatcgcac cacgacgtcc acccgttgca ttaccgaata cccctgcagc tgcacctacg
     1381 aagaagtgcg gcaccacacc aggaataatc acgatcatgt tcagcaaata caacgtgaac
     1441 ataccgatta aacctgcagc aaagctactc aggaaaccaa ccagtacagc gttaggtgcg
     1501 taagggaaga cgaccgggca atcgagagca ggtctggcat tcggaaccag cttgtctgaa
     1561 atacctttga atgccgggac aatctccgca atcaccatgc gcacaccctg cagaatgatg
     1621 tacacgccag ctgcgaaggt aatggattgc atgatagaga acatgaacca gtttttacca
     1681 ccgctaagct ctttcaccgc atctgcaccc gcaaacaggc acgtcaccag gaaaattatg
     1741 ctcatggtaa aagagatagc aaccggcgtg tcgcgcaaga acagcagact tttcggcaca
     1801 ttcatgtctt cagtcgagtg ctctttatta ccaaacttgc tgccgatgaa accggccaat
     1861 acataagata atgttgagaa gtggccgatg gcaacgtcat cagaaccggt cactttcttc
     1921 atgtacggat gcgcaatagc cgggaaaaag accattgcca cgccaacaac caaagagcca
     1981 acggctataa gcgtaatgcc ggtcatgcct gctgttgcca gaattaccgc taccatcatc
     2041 gacataaaca acgtgtgatg accggtcagg aagataaatt tccacggtgt gaaacgggca
     2101 atcataatgt tgataaccat cgcaaagaac ataatcatcg ccatttcttt cccgaagctt
     2161 ttctgcgcta cggaaacaat ggcttcattg ttcggcacta caccctgaat accaaaggca
     2221 tactggaaaa tgtttgcgaa gtcactcagt gacgacacaa ctaaacccgc ccctgcgcct
     2281 aaaatcacaa accccataat ggtcttgacg gtacctttaa tgcattcggt taccggtttt
     2341 ttctgtgcaa tcaggccaat cagggcgatt aaaccgacca gcactgcggg ttcggaaagt
     2401 acatcgctca tcaaaaaacg aaagaaatcc atatcgcccc cagcttacag tgcgcctaat
     2461 tcggttaatg ccacggataa acgttctctc atggctttct tatcaatcat attttccagt
     2521 gcgacgattt tgcctgcgac agactgcgcg actaactgct cggcaatgtc tttggtgccg
     2581 acatagatat cactcggcgt ccctttcgcg gagcccaggt cgacatgatc aacatcagcg
     2641 ctgacggaca aatccttcaa aatgctttta atgctcattt ccatcatcag gctggtgcct
     2701 aagccgttac cgcaaaccac tgtaattttc ataaatgttc ctcaggatta atagcgcgca
     2761 ataacagaca aaatgtcctg cgtcgttttg gccgtcaaaa tagcctgtat gtctttttcg
     2821 ttatcgaata acttggcgag ttgagaaatc gcttcaatgt ggctggtact gtcggttgca
     2881 gccagcacaa tcagtaattt cactggatcg ttttcatcgg catcaaaatt cacacctgaa
     2941 gggatcaggg tcagtgctaa tgacagcttg ttagccccct cttccgggcg cgcatgaggc
     3001 atcgcaatac cagggccaac cacatagtac ggaccgatag tgtcgtgaga gtgatagatc
     3061 gcatcaacat aacgtggttc gatagaaccg ttttcaatca acggtcggca tgaaatcgcc
     3121 acggcttccc gccagtcttt gacctgttcc acgacctgaa cgacctttgt atttaataga
     3181 ttttttaaca tgcgactgat aactcccgat gataattaac aggagagtag atattttacg
     3241 cattaattaa tgtgatgatt gtcgcatttg atagcgctat ctgataacgc taccgatctt
     3301 tgtgatagcg ctatcatttc tctgccgcca tgctttggct atattcggtt cctgcatata
     3361 caatgcaaca ggatatttgc taccgtgggc aaaaactgga aagagtaatg gtaaaataaa
     3421 agtaatgaaa acagctggtt gtaattatcc atggagtcga aatggaaaaa acacgtaagc
     3481 gccgtggtac cggacgggtg acgctgcagg aggtcgctaa ctttgcaggt gtcggaacca
     3541 tgaccgtttc ccgcgcgctg agaacaccgg aacaggtttc tgacaagctg agggaaaaga
     3601 tagaacaggc cgtagaagag ttgggttata ttcccaacag aactgctggc gcccttgcat
     3661 ctggtcacag tcatacagtc gccgtactgg ttccttccct gacagataaa accagctccc
     3721 gctttatgca gtcgttgcag caggtgctta ataaaaatga attccagcta ctgcttgggt
     3781 gtcatgagta taatcagctc aaagaagctg aaatcctgat gacgcttctt caaggtaacc
     3841 cggcggcgtt agttattttt ggttctcagt tggctgataa aactcaccag atactcgaga
     3901 aaacaaatat acctaccatt aacgttgtcg gttccccttt cagtccggct caaattacca
     3961 tcgaaacagc attttttgaa gcatcacata aactgaccga acatatgctg gaacagggtt
     4021 ataagaatat cggatttatc ggtgcgcata tggacaaccg cctgcaacgt cagcaactga
     4081 acggctggca taaagcaatg ctgagctatt accagaactc tgatcttgtt atcaccatgc
     4141 cgggtgccgc aagtttgcaa ttggggcgtt acgcattaaa cgagatatta ctgcgacaac
     4201 cagagctgga tgctgtgatt tgcagccacg aagagattgc gctgggtatc atgtttgagt
     4261 gccagcggcg tttgttaaaa atccctggaa atatcgctgt agcttgtctt gacggttcag
     4321 acagttgcga tcaaacacac ccaaccttga catccatccg tattgactac aaaaaaatgg
     4381 ggacagagac gggtaagctg ctgattggac tattgaataa taatcatgat gaatcagaag
     4441 agtcgcgtat cgtgcaattc aactaccaga tagaattacg tcagagtaca tgagctcaaa
     4501 aggggattat tgcaaagtta gctattgtgg tagggatagc cgtttccggc tattccccgc
     4561 gcagagccgg atgtgtgctg ctaacgcatc ctgtcaacga cggatgaaaa gttatccact
     4621 tatatctcca ccaacggccc aatattgtac cgttcgtgac acatccattt ctacttcagg
     4681 tatttggcgt agtagcgggt gctaaaaatt tgccgcaaaa ccgcaatatc agcccgcaga
     4741 ggcgctctcc agattatcca ttcctatatt atctctgcac cggatgaatt actcgaacaa
     4801 ctcagaaata tgacacatat gcaacttatc aggactctgg gatcctggag gcctgatgcc
     4861 agtgaataca gtaatgttac caacgtttat cgcattacat tgaaatccct tgttcgacgc
     4921 tatttcgagt ttcatgatga aatcaccaac ttggatgtca tgattgcggc aattgttgat
     4981 gaactggcac ctgaactgat taaacgtaat gctgttggat acgaaagcgc tttgcagttg
     5041 ttgatctctg ccggagacaa tccctaacgg ttaagaaccg aatcaggttt tgcggcgttg
     5101 tgcggtgtca gccagtttcc cctatcttct ggaaaaacga accattaccg acttaatcgg
     5161 ggtggggagc gtgttgcaaa tagtgcactt tacatcatca ctataggatg tttacaaact
     5221 gacgataaaa caaaggaata tgtctccagg cgagtagccg aaggacatac aaaaatggaa
     5281 gcaatacgct gtctgaagcg ctatatctca cgcgaagttt atacattact acgaaatcaa
     5341 aacaggcatg ttaactgcat cccgataacg tcttgactct tagaagggcg tccaaggcag
     5401 tcattattcg agcaggcagt tccggcagtt attgtgtcga taccagatca ggcagagtat
     5461 gagccggcgc ggaaaatgct gggataacag ctcaatggaa cgcttcttca ggagtctgaa
     5521 gaacgaatgg atgccggtgg tgggttacgt aagcttcagc gaggcagctc actccataac
     5581 ggacgatatc gttggatatt acagcgcact aagaccgcac gaatataaca gtgggttatc
     5641 cccaaacgaa tcggaaaatc gatactggaa aaactctaac tcggtggcca gtttttgttg
     5701 accacttcag ccatcgccag acgct
//
LOCUS       DAZKFU020000278         5638 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZKFU020000278 DAZKFU020000000
VERSION     DAZKFU020000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.280"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1952
                     /gene="iha"
                     /locus_tag="ACHOQQ_RS21195"
                     /old_locus_tag="pgaptmp_004239"
     CDS             <1..1952
                     /gene="iha"
                     /locus_tag="ACHOQQ_RS21195"
                     /old_locus_tag="pgaptmp_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU4472419.1"
                     /translation="VISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGM
                     PASYTLILIDGVRQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAM
                     GGVVNIITKKNADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGST
                     QQRQGSSVTSLSDTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQ
                     DGQLGSLTGGYDHTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKS
                     DKWGLAGKPRELKESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFR
                     QKSWSVFAEDEWHLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTT
                     GYKAPTMGQLHKGISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGF
                     ITDFSNKIVSYSIDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQR
                     DGDNKGAPLSYTPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKV
                     YDEKGEYLKAWTVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSSLYAGDY
                     FQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(2170..2320)
                     /locus_tag="ACHOQQ_RS21200"
                     /old_locus_tag="pgaptmp_004240"
                     /pseudo
     CDS             complement(2170..2320)
                     /locus_tag="ACHOQQ_RS21200"
                     /old_locus_tag="pgaptmp_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2814..3056
                     /locus_tag="ACHOQQ_RS21205"
                     /old_locus_tag="pgaptmp_004241"
     CDS             2814..3056
                     /locus_tag="ACHOQQ_RS21205"
                     /old_locus_tag="pgaptmp_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472420.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            3347..3715
                     /locus_tag="ACHOQQ_RS21210"
                     /old_locus_tag="pgaptmp_004242"
     CDS             3347..3715
                     /locus_tag="ACHOQQ_RS21210"
                     /old_locus_tag="pgaptmp_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472421.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            3719..3931
                     /locus_tag="ACHOQQ_RS21215"
                     /old_locus_tag="pgaptmp_004243"
     CDS             3719..3931
                     /locus_tag="ACHOQQ_RS21215"
                     /old_locus_tag="pgaptmp_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472422.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQTFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEAESS"
     gene            3928..4200
                     /locus_tag="ACHOQQ_RS21220"
                     /old_locus_tag="pgaptmp_004244"
     CDS             3928..4200
                     /locus_tag="ACHOQQ_RS21220"
                     /old_locus_tag="pgaptmp_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HGU4472423.1"
                     /translation="MTDITDRHTLRRMSWSELTTAAQEAELNRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNPRGRSVCLRPQHPRR"
     gene            complement(4509..5340)
                     /locus_tag="ACHOQQ_RS21225"
                     /old_locus_tag="pgaptmp_004245"
                     /pseudo
     CDS             complement(4509..5340)
                     /locus_tag="ACHOQQ_RS21225"
                     /old_locus_tag="pgaptmp_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5398..5586
                     /locus_tag="ACHOQQ_RS21230"
                     /old_locus_tag="pgaptmp_004246"
                     /pseudo
     CDS             5398..5586
                     /locus_tag="ACHOQQ_RS21230"
                     /old_locus_tag="pgaptmp_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1490 a         1359 c         1414 g         1375 t
ORIGIN      
        1 ccgtaatcag tcaggaggaa ttgcagtcca gccagtacca cgatctggcg gaggcactga
       61 gagctgtgga gggtgtggat gttgaaagtg gtacgggtaa aaccggtggg ctggaaatca
      121 gcatccgtgg aatgcccgcc agttacacgc tgatactgat tgatggtgtc cgtcagggcg
      181 ggagcgggga cgtgacgcct aacggttttt ctgccatgaa tacagggttc atgcctcctc
      241 tggccgcgat tgagcgtatt gaggttatca gggggccgat gtccacactg tatggctctg
      301 atgcgatggg tggtgtggtg aatatcatta ccaaaaagaa tgcagacaaa tggctctctt
      361 ccgtcaacgc cgggctgaat ctgcaggaga gtaacaaatg gggcaacagc agccagttta
      421 atttctggag cagtggccct cttgtggatg attctgtcag tctgcagatc cgcggcagca
      481 cacagcagcg tcagggctca tcggtcacat cactgagcga cacatcagcc acgcgtattc
      541 cttatcccac ggagtcacag aattataatc ttggtgcgcg tattgactgg aaggctacgg
      601 agcaggatgt gctctggttt gatatggata ccacccgaca gcgttacgat aaccaggatg
      661 gtcaactggg tagcctgaca gggggatatg accatactct gcgctatgag cgaaacaaaa
      721 tttcggctgg ctatgatcat actttcacct tcggtacctg gaaatcgtat ctgaactgga
      781 acgagacgga aaataaaggt cgtgagcttg tacgtagtgt tctgaagagc gataaatggg
      841 ggcttgccgg taaaccgcgg gagcttaagg aatctaacct tatcctgaat tcattgctgc
      901 ttacccctct gggagaatct catctgctta ctgtgggggg agagtatcag agttcgtcca
      961 tgaaagatgg ggttgtcctt gccagcacag gtgaaacttt ccgacagaaa agctggtcag
     1021 tatttgctga ggatgagtgg catctcacgg atgtacttgc gctgactgca ggcagtcgct
     1081 atgaacatca tgatcaattc gggggacact tcagtccgcg tgcatatctg gtctgggacg
     1141 tgaccgatgc ctggacgctg aaaggcggtg tgaccacggg atataaagcc cccaccatgg
     1201 gacagttaca taaggggatc agcggtgtat ccgggcaggg acgtactaat ctactcggta
     1261 accccgaact gaagccggaa gagagtgtaa gttatgaggc gggggtatat tacgataacc
     1321 tctccggtct gaatgccaat ataacaggct ttataacaga cttctccaac aagattgtct
     1381 cttattccat tgatgataac accaatagct atacaaacag cggaaaggcc cgcttgcatg
     1441 gtgtggaatt cgccggcaca ttgccgctgt ggtcagagga tgtcactctg tcgttgaatt
     1501 acacctggac ccaaagtgaa caacgtgatg gtgataacaa aggtgcaccg ctgagttata
     1561 cccctgaaca catggtgaat gcgaaactga actggcagat caccgaagag gtggcatcat
     1621 ggctgggtgc ccgttatcgc gggaaaacac cacgtttcac ccggaattat tcgtcactga
     1681 gcgctgtaca gaagaaagtg tatgatgaga aaggagaata cctgaaagcc tggacggtgg
     1741 tggatgcagg tctgtcgtgg aagatgacgg atgccctgac gctgaatgct gcggtgaata
     1801 acctgctcaa caaggattac agtgacgtga gcctgtacag tgccggtaag agttcgctgt
     1861 atgccggtga ttacttccag acgggatcat caacaacagg atatgtgata cctgagcgaa
     1921 attactggat gtcgctgaac tatcagttct gataataaca aaacgctatc actgacggta
     1981 gaatacgttg ccactgcaac tcctggcgga acagtggcaa cgtattaggt taagtgcatt
     2041 tccgatccgc taatgagatt tcgttaccaa caactaatat cgtcacagga aatgcacgga
     2101 ttatttttaa cttatcattt acatacttgt ccagagtgta agcgcaccgt gaaggacgtg
     2161 gggtaaaaat tagtttacag agagagtgac gttccagggc aacaactctt tcatgcggtt
     2221 ggcaggccag gtgttgatta cactgatcac gtggcgtagc cacgtttccg gctcgattcc
     2281 gttaagtttt gcagctaccg atcaggctgt acatcactgc cgcactatcg ctcgtcatct
     2341 caaagtccgg tctcgtcagc aggaaggtat cattctctcc cgccattttt ccgggggccc
     2401 ggtcagataa gtccctttgt ctatcgctga ctcctggctc ataacccggt tagcagaatg
     2461 cagggtcacc acctcgccac gaccgaatcc ggataagtca attgcacctt ctcaatcgcc
     2521 attttgtcac aattaagttt gataaagcag gtcacctctg cactgaatct gctccagtgc
     2581 ctcattggtc tccgtccccc cctttaaact gccggataag actgctgcac agcgccttcc
     2641 ccggaatcaa atcactttcc ctgtccgcca caatccgctt catgctacct cgtattttgt
     2701 ttcagagacc ggaatctgcc agaaagtgac tacgcgtgca catatcggtt ttctgggtta
     2761 catgcatcgt gttattttct gaaatgcaaa ataataaaaa acaaggacac ataatgaagg
     2821 atgaaataca cagtaccagc atcaacccgg atgttgttga gcagtttctg gcccttatga
     2881 acacacattg taaatcctca gaagaggcgc tcgccatgtt gagtacgctt tgttacaacc
     2941 tgatggtgac atgtggcatc cattccatgg acagccagat gctcaatggg catgggatat
     3001 ccatgcaact tcaggggatt gatattgccg aaaggccagg taagaatatt cactaacctg
     3061 aacagggcct gaaaacgctg tcttttcagg cctctgcagg caaaaactga tgacaacaaa
     3121 atttctgcgt ttgtacccca ccgatgaaaa agacaaaaac tcacaccggg cttctagcca
     3181 gtaaagataa aacccgacgt gtcagcctct acgaaacgcc aacggcatgg tgtatcaggg
     3241 ggcaggagtg ctacagcaaa agtacaggac gccgctgtgg cagccacgac agtctgagca
     3301 gactgcgcct agacagcatt aagcctgttg aatgagtggt ggcaccatga aagataacaa
     3361 cacaaccgcc accccagaag cctggaaaat gcgttttctg gccaacggga catccggcac
     3421 atggttttac actgacgaca ttgcagtcac tgacgcgtgg cttcgcgcgc acccggatga
     3481 tgtggaggtt atccctctct gccggatacc accccgtcag cattcccatg aacaacatat
     3541 tgaagaactc cgcgcagccc tggcccggat gctgcatatg catcatatga tgctggaaac
     3601 cacgcattac agggaaaaca tcaccgatgc ccgttgtatc cgtgaaatgc atgaagctcc
     3661 attacaggcg ctcaatattc tggccagata cccgcataaa aaggacgagg tgtgacgcat
     3721 gtggctactg attttctggc tttcatctcc ctttaatgtc tcttccggtg atgcccggca
     3781 gcccacactg gcggtgcaca gccagacatt tcataccgaa cgggcctgtc gcgatgcatt
     3841 gcgcgccatt aaaaaggaga atgttggcga attttctctg cggggcgttt gtgtttctga
     3901 cggaaacacg aaggaggcag agagctcatg acggatatta ctgacagaca tacacttcgc
     3961 cgcatgtcct ggtcggaact taccacggca gcgcaggaag cagaattaaa ccgtgattac
     4021 gagcgtgcca gaatagtgtg gtcattcgcc ttacatgttg caacaaccac gattaacaaa
     4081 aatctggcca tagctcatat tcgacgctgc gatactttgt tgcataaatc aaaaacagta
     4141 cccggaaaca acccgagagg ccggtcagtg tgcctgagac ctcaacatcc ccggagatga
     4201 ctgacattcc ggactgacgc agttgctgca tatctgtggc aatacgttgt atccagccag
     4261 acaggctatc ccgcgtagtg acaattaacc cgctttcctg caccagcttc agccgggccg
     4321 tcggggattt taccagccgg aaatatcagt aaaacaacat atcattatgc catggtgtac
     4381 gcggccagtg tatgcttact taaggaatga gggataaatt ggatattatt acttcacttt
     4441 gcttttttat gagattcatc acataagtgg ggttttactg ttaaataatc tgaatttatt
     4501 tgatatccgt aaagcagatg tctcagttca ctgtttccaa cttttgttat tcctctgggg
     4561 cgaatgctgt ttccgctgga atattcacct ggcaccagtc ccagaaaagc catgagctgt
     4621 tcaggattgg aaaatcgcga aaaatcaccc agttcagcaa ccagggttat tgcgattagc
     4681 tgaccaacac ctttaagagc ctgcaactgg cagaccagat tgcacagaga tcactcaggt
     4741 aacaggcgag atatttcctg atcgagttga agtatgcgat cttcaatctg ctccagattc
     4801 tgacaataat gttgaaagca atctgttgag atagcagacc aaatgattga ttaactaacc
     4861 aggttcgatg tttgccggtt cagtgcttcc tggcataacg tttgtctgtt cgtgtcacgt
     4921 tttgcagcgg cacgggctct gattaaatcc cgcatagctt catgagtcag ctcagggagc
     4981 cagactgggg gaacctcacc atccctcagt aacctggcga gagaaattac gtcccgatgg
     5041 tcatttttga tcctgtctgc cggtgatttg ggtatacggg aagggggcaa taaccaggca
     5101 ttttatattc atcactgtga gttgatgata tagcccaaac ccacagggac caccttcata
     5161 gcaggaaaga atgtttccgt aaggtccctg caatttctga cataatcgat gtacagccaa
     5221 ggcttcgttg ttaatcgtac cataataacg aacttcgcca agccattcag gtgtagcaat
     5281 cgccaaggtt tctttatgaa catccagccc gacgtaagct gcaatatttt ccgaatccat
     5341 gatgacctct ggagtatccc accaatgcta tagtgagtat ataaaggtca tatcatctac
     5401 gtgttcttcg gcaacgatca cagtggagag cgtggagcgc tactgtacgg gctgattggc
     5461 acctgccgac tgaacagtat cgatccggaa gcgtatctgc gccatattct gagcgtactg
     5521 ccggaatggc cttccaaccg tgttgacgaa ctgctgccat ggaacgtagt tctcaccgat
     5581 aaataagcgt caatacggca ttcgattaat gcacttacag ctaaatggag tttttgaa
//
LOCUS       DAZKFU020000279         5629 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZKFU020000279 DAZKFU020000000
VERSION     DAZKFU020000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.281"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..508)
                     /gene="sfmF"
                     /locus_tag="ACHOQQ_RS21235"
                     /old_locus_tag="pgaptmp_004247"
     CDS             complement(<1..508)
                     /gene="sfmF"
                     /locus_tag="ACHOQQ_RS21235"
                     /old_locus_tag="pgaptmp_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGU4472424.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADKDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVA
                     KADAIFMIN"
     gene            complement(519..1526)
                     /gene="fimH"
                     /locus_tag="ACHOQQ_RS21240"
                     /old_locus_tag="pgaptmp_004248"
     CDS             complement(519..1526)
                     /gene="fimH"
                     /locus_tag="ACHOQQ_RS21240"
                     /old_locus_tag="pgaptmp_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4472425.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(1539..4148)
                     /locus_tag="ACHOQQ_RS21245"
                     /old_locus_tag="pgaptmp_004249"
     CDS             complement(1539..4148)
                     /locus_tag="ACHOQQ_RS21245"
                     /old_locus_tag="pgaptmp_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4472426.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(4179..4871)
                     /gene="fimC"
                     /locus_tag="ACHOQQ_RS21250"
                     /old_locus_tag="pgaptmp_004250"
     CDS             complement(4179..4871)
                     /gene="fimC"
                     /locus_tag="ACHOQQ_RS21250"
                     /old_locus_tag="pgaptmp_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4472427.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(5091..>5629)
                     /gene="fimA"
                     /locus_tag="ACHOQQ_RS21255"
                     /old_locus_tag="pgaptmp_004251"
     CDS             complement(5091..>5629)
                     /gene="fimA"
                     /locus_tag="ACHOQQ_RS21255"
                     /old_locus_tag="pgaptmp_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4472428.1"
                     /translation="LRFISSALAAALFTATGSYAAVVDGGTIHFEGELVNAACSVNTD
                     SADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAINDNL
                     LAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGTGT
                     SASAGQANADATFIMRYE"
BASE COUNT         1572 a         1362 c         1221 g         1474 t
ORIGIN      
        1 aattgatcat aaatatcgca tccgctttcg ccacacctgg ctgaacaccg ctggctaacg
       61 ctcgataatt ggcaaaaaag tttagtgtga cattgccatt tgcatctact tcctcagtcg
      121 ggctcgcctc ccccagtgcg agccgggagc gatcgctatt acgtaattcg atggcaacgg
      181 tttgtgccat tgcgggatca tccagagcca gcaggttggt atcggatgcc ggcgttcccg
      241 taaataaaat cgcaactgaa cccggaggac atccctccag ccgcaggcta aaagggacga
      301 gtgccgtggt atcgccagcg ttcagtaatt gtgtcgtagg ccatctgcct aaatctaccg
      361 tcttatcctt atccgctgtg tttacggtac aagagaaatc aaccacgtta ccgtgcaaat
      421 tgatattaat cgttcctaaa gggtcagctg cccatccact ggaactccac agtaacccgc
      481 agaaacagct aaagaatact cttctcattc tccgtacctc attgataatc gacgcgtaaa
      541 taccccagcg cgctaaacgg cccctcggtc ggtttttgac cggtaatact gataggccag
      601 gcgcgaagtg tgacattggc tgccgcagct gcatccagac ggaaaggaat aacgctattg
      661 agatcgttag gcgtgatcgg cgtatcgttc tgatcggcga caataaaacc taaatcctga
      721 ttgtccgaca ccatcgcctg accagaaacg gcactggctt ccagacgcat tgttaaataa
      781 gcctgcgcag caacattcgt acatttgacc gcaatgctct tggtttgcgg catgacacca
      841 gcaggtcgat tacccggccc tgccgcacta aataacgatg cgccgatatc accaaaatca
      901 aattcaacaa tctgcccggc atttaattcg cagttttgcg gtacttcaac ccggccacca
      961 aaactaatgg tataaacagg tgtaaccaac ggatcgtaag gcgttgatgt cacatacacg
     1021 gtaaacatca cctgtctggg gatctccacc atgttaataa agggccgaat aacctttaaa
     1081 cgaaatatca aacgagaatc catcaccccg aacggaaggt tttgcgaaac gttaggatct
     1141 gttcccatac gaatataatt ttgcggggga aattgaatat ccatcacgct atcaaccaga
     1201 ctcatcgcac ctaatagata atcatttaat tgcatatatt tataattatc gatagtttcc
     1261 tgaacaataa agttggtgac ataactacgg tatgtataat tcaccagcgt gccgggtggg
     1321 caaattgctg acacacctac ccagccggat ttttccggta aaacaacaat ctgccctggc
     1381 tggttattgg tgctattaaa gacatttgag agatcatagt gttcatcttc tgctacgcca
     1441 gtactatttg cacaaacagt agcccaggat atgtttgcca gacacaaaca tgccattgcc
     1501 aataataatc tgcagattat cttcattgat aatttccttt atgagccagg atgtgtgcaa
     1561 actgccgaaa taacagtgac ggcttgctgt aagctttgct tcggcaatac atagtgcgca
     1621 atgcagcgtg agttcgcgcc gtctccccac tgaaccagta attcaccaga caatggcgca
     1681 ccacttaaat aaacttgccc gtcatcaccc accatactgg ttattccggt actgttttcc
     1741 cgtaccagtg atccaaacgg caccggtttt ccgccctggg taacggtaat gagcgcccgc
     1801 acgcctatgc gggtatcaaa attggcacga accaacgcgc cttgcgtcgg cacaacgctg
     1861 ctaatatttt tttcaacatc gatggaattc cccatcgtat tggtatcaag cgcgatacgg
     1921 ttataccgat aaaccgtggc atacggcatc accgcatagc cgcgccagtc ggttaaaatg
     1981 ccagtttgat tttcaatgcg tacaccgcct gcgccaggcg ctttaatcaa aacattggta
     2041 tcccctaaag gctggctcag cgttatgcca ttttcatgcc cgaccactcc gccagacagc
     2101 tgccagttaa cgtcatgttg atcgcggtcg tagttatacc cgactcccag cgtaccgtaa
     2161 gcggcctgcc agtttgccgt ggcgctgccc gtatacccat tattcgaggt atcaccctgg
     2221 cttacgtgat aactcaggtt gtggccttcc agtaaggtcc cacctacacc tgccagccag
     2281 ctattttgcc cgttgctgtt acggttggcg ttaaaggagg cataagcgcg gtcgagcgca
     2341 ttttcccggg tgtagcgacg tttggtcaaa acattgaaag gcactgaaac attaagtccg
     2401 acaatacgtt cgttatcggg gatccctaca gattcattcc acgaaaatga gagcgaataa
     2461 ctgatgccaa cccagctgct ggtatacccc acctgatacc acgtatctga atccgaagta
     2521 ttccagtatt tttgatgggt accagaaata taaagcgagc caaagtcatt aagtgattgt
     2581 gaaatattta actgcaaacg gtctttacgg ctaaagcgta aattgtggta attcacgatt
     2641 atcggttcat cccgatgctc accgtcgtaa tcgtaatcat attcgtaccc ctccattcga
     2701 cgatacgcaa catcatcaag ggtataaaaa ccttgtgtcg aatagcggta acccattaac
     2761 tgaaaattgg tgccgaaggt gttcatcgat ttcgcataga ggaagcgaat ggaatccccc
     2821 tcatgacgac tgtcgtcggc taactggctg cgcgcatggg tcacatccag cgacactgcg
     2881 ccccagttcc cgaggttgcg ccccagccct aataaaaagg cggtgtaatt ggcagataat
     2941 tgcgtcccgc cgtaggcagt aaattcctgt ggtaaaccgc cgagtgccgt gccctgaaaa
     3001 aagaaaggcg atgattgctg actattaccg ctacgaaaat cgcccgccgt caggtcaaat
     3061 ttgaaacgcc cttcgcgttg taaaattggc actgttgaat acggaattgt gtaattctgc
     3121 tgattaccat cgcgctcgtc gatggtgacg tccagatcgc catttgaaga tgtcgggtgc
     3181 aaatctgtaa tttcaaaagc gccgggggaa acatagcttt gatagataat aaaaccattt
     3241 tgccgaatcg ttagctgggc cgccgtacgg gcaatcccac gtaccgttgg ggcaaaccct
     3301 tgctggctat caggatacat attatcggaa gaataaagcc gtacaccacg aaatccaaca
     3361 ccatcgaaaa tatcacttcc tgtattgccg tctcccatta ccagttcact tttcagcgga
     3421 ataatggcgc gctgtaccca ggtgccaata ttattccact gttctgaatg atatccattt
     3481 ccgcgaaaat agttccagga accattgttg cgtaaacgcc acgggccaat attaatcccg
     3541 ctgagctcac taaaaaaata actatcgtta ccattacctc tgttaccagt gaaattataa
     3601 ttcaggagta aagcaggaat accttcatcc cactcttcag gcggaatgta accgtgagcg
     3661 ctactcaaca acgctatctg aggaatagtg atgttcaggc gcatcgctgc aaaatcaaag
     3721 ttaattgtgg catcaggtac agctttcagt aaattgatgc atttattgtt ttgctgctgg
     3781 gctaactcgg gaaatgcact gctattaagg ccaattcgtt caaggagtac ctggttaaaa
     3841 cagggcatta acccacctga tttatcacct gtattttctg ttgtcgattc aaatacgata
     3901 tcctgtgaac caatgaactc atcattacgc cagagatcta cccgatacac gcccgcaggt
     3961 tgatgattac ctctctcaaa gcgcgataaa tcagcaacgg atgtgccatt ctctaataaa
     4021 aatgccgggt tgaaataact ttccgcatag cttaaaaagg agggtaaaat ggcaagagat
     4081 gaataacaaa ttccggccag acaccaggta tacctttgcg gaatatccgt agtagtgggt
     4141 attttcataa ttagctctga tattgtcaga tgattcggtt agtttaagtt cacttctctg
     4201 accggggtta ctgaaccata atcattaacg gtctgaaatt tcagctttcc ctgcactcct
     4261 gagggtaagg gaatttgtga attaactctg ggtgcagcca tagcattccc caacttttgg
     4321 ctgcccactt gtaagttaac cagggtgaca taaaaaggtg atggattatg aacattgata
     4381 tagttaccgg atcgcgaaaa cttgagtgtg tccggcgctt ctgcgggtaa ttcttgtaat
     4441 tgaattggac ggagaaataa tttcatgcgc gataaaatcg ccagttgcaa aacattcctg
     4501 ccgttcaatg catttttatc taccgaaggg atcgtcttaa cattcatcca gaacagagac
     4561 tcacgatctg ctgccagcgg tggaccggtg taaataatac gcaaagtatt ttcgcttttg
     4621 ggttcactaa caaatagtgg cggtgtaatg atgaatgact tttcttttac accgctgctg
     4681 ttttcaatcc acgaattgac cagaaaacgc tcattggtat ggctatttct aatccatacc
     4741 gcagtctgtt tagcatcagc gggataaata atacgcgtgg cacctaatgc gatccctccg
     4801 gcagcatggg cgctggccga aatgattaaa taaaatataa tgagcatcaa taactttatt
     4861 ttagtcatca tagttaatac gccttgtgta ttcctgacgg caaaaataag aaatataaag
     4921 aaatgattgc tttggtttct tacaacacat cattcccttt tcatttaata aagcggcatg
     4981 atacaaagtg aacatgattt cgatttacat tgccaccata ccgcttcaga caaccggtac
     5041 attactttgt tataagacgt gatatataat tcaaaacaac gtggttttga ttattcgtat
     5101 ctcataataa aagtcgcgtc agcatttgct tgccctgctg atgcacttgt accggtgcct
     5161 ttataacgtg cagaaaaatg aagaacgttg gtcccgggga tcaagttctg gttggttgag
     5221 aagctattcc catcaggttt gagaattgcg gatgtattat caagtatttc aataccgaca
     5281 cccgttgctg ttgttgtatt ggtactggat gcaatggcca ataaattatc attgattgca
     5341 tcagcctgac cagaaaatgc aacggcagca ttagcggcaa caacaggatc gcagtcgttc
     5401 aactgtatag tgaatggaat taatgcagag gtattaccaa cagcattgaa aatatcggta
     5461 cgatattgac cgagtgtgac aacctggtct gccgagtcag tattcactga acaggcagca
     5521 ttcaccagtt cgccttcaaa gtgaattgta ccgccatcta caacggcagc ataactaccc
     5581 gtagcggtga atagtgcggc agccagcgca gacgaaataa atcttaatt
//
LOCUS       DAZKFU020000280         5626 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZKFU020000280 DAZKFU020000000
VERSION     DAZKFU020000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.282"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..404
                     /locus_tag="ACHOQQ_RS21260"
                     /old_locus_tag="pgaptmp_004252"
     CDS             <1..404
                     /locus_tag="ACHOQQ_RS21260"
                     /old_locus_tag="pgaptmp_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU4472429.1"
                     /translation="WQQHTLKDKHYASLLTMLDLNEASESDKSKIFFCLSAVFANISH
                     SNVFNGIPDASKTLKRYAFALLAKAHSLDESMISNQTFNTYKTVLLDFNNLSNEEANQ
                     LRISSLYRDMVRYAQYRFSKVLSEWTPDAWL"
     gene            688..2838
                     /gene="dinG"
                     /locus_tag="ACHOQQ_RS21265"
                     /old_locus_tag="pgaptmp_004253"
     CDS             688..2838
                     /gene="dinG"
                     /locus_tag="ACHOQQ_RS21265"
                     /old_locus_tag="pgaptmp_004253"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGU4472430.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2866..3828
                     /gene="ybiB"
                     /locus_tag="ACHOQQ_RS21270"
                     /old_locus_tag="pgaptmp_004254"
     CDS             2866..3828
                     /gene="ybiB"
                     /locus_tag="ACHOQQ_RS21270"
                     /old_locus_tag="pgaptmp_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGU4472431.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3969..5054
                     /gene="hcxB"
                     /locus_tag="ACHOQQ_RS21275"
                     /old_locus_tag="pgaptmp_004255"
     CDS             3969..5054
                     /gene="hcxB"
                     /locus_tag="ACHOQQ_RS21275"
                     /old_locus_tag="pgaptmp_004255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGU4472432.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDGGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            5121..5180
                     /gene="ybiE"
                     /locus_tag="ACHOQQ_RS21280"
                     /old_locus_tag="pgaptmp_004256"
     CDS             5121..5180
                     /gene="ybiE"
                     /locus_tag="ACHOQQ_RS21280"
                     /old_locus_tag="pgaptmp_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGU4472433.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5283..5543)
                     /gene="ybiJ"
                     /locus_tag="ACHOQQ_RS21285"
                     /old_locus_tag="pgaptmp_004257"
     CDS             complement(5283..5543)
                     /gene="ybiJ"
                     /locus_tag="ACHOQQ_RS21285"
                     /old_locus_tag="pgaptmp_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGU4472434.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT         1359 a         1373 c         1570 g         1324 t
ORIGIN      
        1 aatggcaaca gcatactctg aaagataaac attacgctag tctattaacg atgctggatc
       61 tcaacgaggc cagtgaaagt gataagtcta aaatattttt ctgcctgtct gccgtatttg
      121 caaatatttc tcacagtaat gtctttaacg gtattcctga tgcgtcaaaa actttaaaac
      181 gttatgcatt tgctttattg gcgaaagctc attctttaga tgaaagtatg ataagcaatc
      241 aaacttttaa tacatacaaa acggtattgc ttgatttcaa taatctgtct aatgaagagg
      301 ccaaccagtt gcgcataagt tcgttatatc gtgatatggt ccgctatgca caatatcgtt
      361 tttctaaagt tttatccgag tggacgccag acgcatggct gtaaagatcg cagcagaata
      421 atggcattga tggccctggc tcaggccagg gtcggcaata ccggttcctg cgaagcgatg
      481 acggcggcaa gtgtaaccac gaaatgcggt atcacttgct gttttctgcc ctgaagactg
      541 gcgctcactg tgccgtcgaa ttttcaatca aatatattgc gagagggcgt gtttgcgagc
      601 cgctttccag aaacagaaaa accattaccc ctgaaaaccg aaaaatgcca caatattggc
      661 tgtttataca gtatttcagg ttttctcatg gcattaaccg ccgcgcttaa agcgcaaatt
      721 gccgcctggt ataaggcgct tcaggaacag atccccgact ttattccccg tgcgccgcag
      781 cggcagatga tcgcggacgt cgccaaaacg ctggccggag aagaagggcg gcatctggcg
      841 attgaagccc ccactggcgt tgggaaaacg ctctcctatt tgattcccgg catcgccatt
      901 gcccgcgaag agcaaaaaac gctggtggtg agcaccgcca acgtggcatt gcaggatcag
      961 atctacagca aagatttacc gttgctgaaa aagatcattc ccgatcttaa attcactgcc
     1021 gcttttgggc gtgggcgcta cgtttgcccg cgtaatctga cggcgcttgc cagcactgag
     1081 cccacgcaac aggatctgct ggcgtttctt gacgacgaac tgacgccaaa taatcaggaa
     1141 gagcaaaaac gttgtgcgaa gctgaagggc gatctcgaca cttataaatg ggatggtctg
     1201 cgcgatcata cggatatcgc tatagatgac gatctctggc gtcgtttaag taccgacaaa
     1261 gccagctgcc tcaaccgcaa ctgttactac tatcgcgaat gcccgttttt tgttgctcgt
     1321 cgggagattc aggaagcgga agtggtggtg gcaaaccatg cgctggtgat ggcggcgatg
     1381 gaaagcgaag cggtattgcc tgacccgaaa aatttactgc tggtgctgga cgaaggccat
     1441 cacctgccag atgtggcgcg ggatgcgctg gagatgagtg ccgaaatcac cgcgccatgg
     1501 tatcggctac agctggactt gttcacgaaa ctggtcgcta cctgcatgga gcagtttcgc
     1561 ccgaagacca tcccgccgct ggcgatccct gaacgtttga atgcgcattg tgaagagctg
     1621 tatgagctta tagcctcatt aaacaacatt ctcaatctct acatgcctgc tgggcaggaa
     1681 gcagaacacc gttttgcgat gggcgaactg cctgatgaag tgctggagat ctgccagcgg
     1741 ctggcaaaac tcaccgagat gctgcgtggt ctggcggagt tatttcttaa cgatttaagt
     1801 gagaaaaccg gcagccatga cattgtacgt ctgcatcggt tgattttgca gatgaaccgc
     1861 gcgttgggga tgttcgaggc gcaaagcaaa ctctggcggc tggcttcgct ggcgcaatct
     1921 tccggtgcac cggtgaccaa atgggcgacg cgggaagagc gcgaagggca gctacacctc
     1981 tggtttcact gcgtgggaat acgtgtcagc gatcagctgg aaaggctgct gtggcgcagt
     2041 attccgcaca ttattgtcac ctccgcaacc ttgcgttcgc tgaacagttt ttcgcgtttg
     2101 caggagatga gcgggctgaa agagaaagcg ggcgaccgtt ttgtggcgct ggattccccc
     2161 tttaaccact gcgaacaggg caaaattgtt attccccgga tgcgcgttga gccttccatc
     2221 gacaacgaag aacagcatat tgctgaaatg gcggcctttt tccgtgagca ggtggagagc
     2281 aaaaaacatc tcggtatgtt ggtgctgttt gccagcgggc gtgcgatgca gcgctttctc
     2341 gactatgtga cggatttacg tctgatgttg ctggtgcagg gcgatcagcc gcgttaccgt
     2401 ttagttgaac tgcaccgcaa acgcgtcgcc aacggtgagc gtagtgtgct ggtgggctta
     2461 cagtcatttg ccgaagggct tgatttgaaa ggtgatatgc tcagccaggt gcatatccac
     2521 aaaatcgctt ttccgcctat cgacagcccg gtggtgatca ccgaagggga atggctgaaa
     2581 agcctcaacc gctatccatt tgaggtgcaa agcttgccga gcgcctcgtt taacctgatt
     2641 cagcaggttg ggcgactgat tcgaagtcac ggttgctggg gcgaagtggt tatctacgac
     2701 aaacgcttgc tgaccaaaaa ttatggcaag cggctactgg atgcattacc ggtatttccg
     2761 atagagcaac cggaagtccc tgaaggtata gttaaaaaga aagaaaaaac gaaatcccca
     2821 cgccgtcggc ggcgttaatg atgtaagtca ggtaaggagt cgtaaatgga ctatcgcaaa
     2881 atcattaaag agatcgggcg cgggaaaaac cacgcgcgcg atttagaccg ggatactgcg
     2941 cgcggtctgt atgcccatat gctcaacggt gaagtccctg accttgagtt gggcggtgta
     3001 ttgattgcgc tgcgtatcaa aggggaaggg gaagcagaga tgctcggctt ttacgaagcc
     3061 atgcaaaatc acaccatcaa gctgacgccg cctgcaggca agccgatgcc gattgtcatc
     3121 cccagttaca acggcgcgcg taaacaggcc aacctgacgc cgttgttggc gattctcctg
     3181 cacaaactcg gttttcctgt ggtggttcac ggggttagcg aagatccaac tcgcgtgctg
     3241 accgaaacca ttttcgaact gatgggtatt acgccaacgt tgcacggcgg gcaggcgcag
     3301 gcgaagctcg acgaacatca accggtgttt atgcctgtcg gcgcgttttg cccgccgctg
     3361 gaaaaacaac tggcgatgcg ctggcggatg ggcgtgcgta acagtgcaca taccctggcg
     3421 aaactggcga cgccatttgc cgaaggtgag gcactgcgtc tttccagcgt ttcgcatccg
     3481 gaatacatcg ggcgagtcgc gaagttcttt agcgatatcg gcgggcgggc gctactaatg
     3541 catggtactg aaggtgaagt gtatgctaat ccgcagcgct gcccgcagat caatctcatt
     3601 gaccgtgaag ggatgcgggt gctgtatgaa aaacaggacc ctgctggtag cgagttattg
     3661 ccacaagcaa aagatccgga aaccacggcg cagtggattg agcgttgcct ggctggcagc
     3721 gaaccgattc ccgaatcgct gaaaatccag atggcttgct gcctggtggc tacgggtgaa
     3781 gcggcaacta tcagcgatgg cctggcgcgc gttaatcagg cattttaatt tttatctcag
     3841 ccggatgagc cacgtcatcc ggcttttacc ccaccgcaat atgaaattcc tgcatcttta
     3901 ttgaccttct cgcgcccggc gtgcagcata aaaaaacaac aaacacataa cataaacagg
     3961 agttaaccat ggaaagtggt catcgctttg atgctcagac gcttcacagc tttattcagg
     4021 ctgtatttcg tcagatgggt agcgaggaac aagaagcgaa attagttgcc gatcatttaa
     4081 tcgcggcaaa cctggcaggg catgattcac atggtattgg catgatccca agctatgtac
     4141 gctcctggag tcaggggcac ctgcaaatta accatcatgc caaaatcgtt aaagaggcgg
     4201 gggcggcggt cacgctcgat ggcgatcgcg catttggtca ggtcgcggca catgaagcga
     4261 tggcgctggg gattgagaaa gcgcatcagc acggtattgc cgccgtggcg ctacataact
     4321 cgcatcatat cggccgtatc ggttactggg cggagcagtg tgcagcggcg gggtttgtct
     4381 ctatccactt tgttagcgtg gtcggtattc caatggtcgc gccgttccac ggtcgcgaca
     4441 gccgctttgg caccaatccg ttctgtgtgg ttttcccacg taaagatgat tttccgctgt
     4501 tgcttgatta cgccaccagc gccattgcat ttggcaaaac tcgcgtcgcc tggcataaag
     4561 gcgtccccgt gccgccaggt tgtctgattg acgttaacgg cgtgccgacg acaaatccgg
     4621 cggtaatgca ggagtcgccg ttgggttcgc tgttgacctt tgccgaacat aaaggctacg
     4681 cccttgcagc gatgtgtgaa attcttggcg gggcgctttc tggcggtaaa acgacgcatc
     4741 aggaaacgtt acaaaccagt cccgatgcca ttcttaactg catgaccact atcatcatca
     4801 atccggaact gttcggcgcg ccggattgta gcgcgcagac cgaagccttt gccgagtggg
     4861 tgaaagcctc gccgcatgat gacgataagc cgattttgct cccgggcgag tgggaagtga
     4921 acacgcgtcg cgaacggcag gagcagggga ttccattgga tgggggaagc tggcaggcca
     4981 tttgtgatgc ggcgcggcag attggtatgt cggaagagac gttgcaggct ttctgtcagc
     5041 agttagccag ctaaaaaaaa gcccgtccag tggcggacgg gcaaacaagg gtaacatagg
     5101 atcaatgagg gttagagcat atgcgtctgt cggcaaacag acagggaaat acttgtgctg
     5161 gacgtagcgt aaacgcctga tccgtccaac ggttcggatt ttgtaggcct gataagacgc
     5221 ggtagcgtcg catcaggcat ctgttgccgg atgcggcgta aacgccttat ccgtcctacg
     5281 aattacttat aaattaccgc agtaccgctt aatttattgt tgttggtggc ggaagtgatg
     5341 ctgtatccac tcgccccagc tgccgccgct ttctcagcca gtttcgcttc cagggcatcg
     5401 agggtggatg cgccatcggc agaaaccacg ccgattttat tcatgttctg tgcctgggat
     5461 gccgttaccg gttccgcggc aaacacgcca aatgacagag ttgaaagagc catagcagca
     5521 acaacagtat tgatagtttt cataattaat ctctcgcagg tgatttttgt ataagtgacg
     5581 ttgtttcgtc gatgtgatga gtatcacgtt tttttacgag agataa
//
LOCUS       DAZKFU020000281         5615 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZKFU020000281 DAZKFU020000000
VERSION     DAZKFU020000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.283"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            97..4419
                     /locus_tag="ACHOQQ_RS21290"
                     /old_locus_tag="pgaptmp_004258"
     CDS             97..4419
                     /locus_tag="ACHOQQ_RS21290"
                     /old_locus_tag="pgaptmp_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4472435.1"
                     /translation="MNLRKKHICIAISLVLNPASISQASAQADTNKLTVEVDETISSA
                     GDDWKSIEIGTTKFTAKDESKGINFDHKSPVAEGRNSIAIGTSAEAGGDISTKEGLYT
                     GESIAIGAKTKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGKTYGREKIENNYLA
                     SEAKGDGSIALGSRTFAKGELSVSLGSVSSAEGMASNAIGATAEAKGDMATAVGFNAL
                     ANAGNALAVGSQSSANKNDALAVGSEANAAQNETVALGHGATVTVAKGNVSIGADSRD
                     KQAQKVTEAKVRTKRGAEVTYSGFAGNNAASVVSVGDAGKERQIVNVGAGAVSEKSTD
                     AINGSQLYAIAKTLKDDLDAINPTWTLKDDKGGSATINGGKDNKNGNTLTVKGDGDIA
                     VTVNDKGMTIDGTTLKNEIKNDAIKIKGGDGKTLTKSLGDNVEITGKGPIKTQVAGDK
                     LEISAETASLPVNGGNGKITVNNGDGDKLVTAKNIADAINQSGWKIKADKDTGGVIEG
                     NGQGTETLIKPGDMVTLKAGKNLSVKQSNGDFTFSVIDTPSFSSVQLNGNGKNGNVTL
                     SSEGNKLTLSGGNGAGQNGVVISGVASGLNNKDLAGLKADDAEMKNAASVADLKTAID
                     GVSTSMQGAGFALKDSKGQEVKQSLGQAIKVSGDSNITTTAITANGDKGLKISLNNDL
                     TVGDKAPGSIKVNGADGKEAVSLNGQDGTIKLTSSPAAGGKSASATIAVNAGKGGLTD
                     AAGTTKTRLTYTPDGKSPAEELATMNDGLSFEGDDGKVIHKKLNEKLTIKGGAEKNAE
                     VTDGNIRVDNDNGGLLVRMTRNLRDLASAVFGTGGQITTVNDKGITITTPAAGGGQGD
                     TVSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALRDSSAALVN
                     TGFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRLADDGSVSVG
                     GTRMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVSQLNREISQATAAT
                     TWALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYEIDVTGIPVEYTDSQ
                     GNPLVNIGGRFYTQTEDAATGKLTMTPAEPARVRISSEQPLVLTNVAAGNVSPVSTDA
                     VNGSQLASAARIAGGDNVVWKDGKAALAPDTFSELTTAGGGKTTVNGGVHKTPDNVAD
                     AVNMLNREGTKYFKAASSGQAAKAEGRDSIAVGQGAVSRGQNSIAMGNGAEVTRQAAA
                     GSVAVGSHARAGRANTGTYALNGQAVAGRTGSDTAVVSFGQPGQERQLQSVAPGVLSA
                     NSTDAVNGSQLHATNRQVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAAAMSG
                     IPQAYLPGKSLMGLGVGGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSVGAGV
                     GWQW"
     gene            4444..5223
                     /locus_tag="ACHOQQ_RS21295"
                     /old_locus_tag="pgaptmp_004259"
     CDS             4444..5223
                     /locus_tag="ACHOQQ_RS21295"
                     /old_locus_tag="pgaptmp_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4472436.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGETSVTD"
     gene            complement(5392..5615)
                     /locus_tag="ACHOQQ_RS21300"
                     /old_locus_tag="pgaptmp_004260"
                     /pseudo
     CDS             complement(5392..5615)
                     /locus_tag="ACHOQQ_RS21300"
                     /old_locus_tag="pgaptmp_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1553 a         1123 c         1676 g         1263 t
ORIGIN      
        1 aagaaatgaa ggcagtccgc cttacataat attcatgcat gatttttatg tcattccgac
       61 tgagtggaag aaaataaagg agaaaagagg atatatatga acctcagaaa aaaacatatc
      121 tgtatagcca tatcacttgt tcttaatccg gcatccatat cgcaggccag tgctcaggca
      181 gatacaaata agttaactgt tgaagttgat gaaaccatca gttcggcagg cgatgactgg
      241 aagagtattg aaataggtac aacgaaattt acagcaaaag atgaatcaaa gggaattaat
      301 ttcgaccata aatcaccggt ggctgaagga cgtaactcta tcgcgattgg taccagtgca
      361 gaggctggag gtgacattag cacgaaggaa ggtttgtaca caggcgagtc tattgcgata
      421 ggagcgaaaa cgaaagcaac taaagaacag tcaattgcaa taggtggcga taccaaagct
      481 accggatggg gggctattgt tattggtggg gatgatttaa ctcctttgca tggtaagact
      541 tatggtcggg aaaaaataga gaataactat ctggcatcgg aagcgaaggg ggatggcagt
      601 attgcgctgg gcagcagaac ttttgctaaa ggcgaattgt cagtatcgct tggttcggtg
      661 agttctgctg aggggatggc atcgaatgca attggagcca cggcggaagc taaaggggat
      721 atggcgactg cggttggttt taatgcgctg gctaatgccg gtaatgctct ggccgttggt
      781 agtcagtcct ctgcaaataa aaacgatgct cttgctgttg gttcagaggc aaatgccgct
      841 cagaatgaaa cggttgccct ggggcatgga gcaaccgtta ctgtcgcaaa ggggaacgtg
      901 tccatcggcg ctgactccag ggataagcag gcacaaaaag taacagaggc taaggtcaga
      961 actaaaagag gcgcggaagt cacatatagt ggctttgccg gaaataacgc agcatcagta
     1021 gtatctgttg gtgatgcggg taaagagcgt cagattgtta acgtgggggc gggtgctgta
     1081 tctgagaaaa gtacagatgc cattaacggt agtcagttat atgcgatagc gaagacactg
     1141 aaagatgacc ttgatgccat taatccgacg tggacgctaa aggatgataa gggaggttct
     1201 gcgactatta atggtgggaa ggataataag aatggtaaca ctttaactgt taaaggtgat
     1261 ggagatattg cagttactgt taatgataaa ggaatgacga ttgacggaac aacgctgaaa
     1321 aatgaaataa aaaatgatgc catcaaaatt aaaggtggag atggtaagac gctgacgaag
     1381 agtcttggtg acaatgtcga aatcactggc aaaggcccaa taaaaaccca ggtggctgga
     1441 gataaactgg agatttcagc agagacagct tcgttgcctg taaatggtgg taacgggaag
     1501 attacagtaa acaatggtga tggcgataaa cttgttactg cgaaaaacat tgctgatgcc
     1561 ataaaccagt ctggctggaa aattaaagcg gataaagata ccggtggtgt tatcgaggga
     1621 aatggtcagg gaacagaaac actgataaaa cccggtgata tggttacgct gaaagccggt
     1681 aaaaatctca gcgtgaaaca aagtaacggt gattttacat tctctgttat tgatactccg
     1741 tcattcagca gtgttcagct gaacggtaat ggaaaaaacg gaaatgtaac gctctcatcc
     1801 gaaggtaata agctgacatt gtcaggcggt aatggtgccg gacaaaatgg tgttgttatc
     1861 agtggtgtgg caagtggatt gaataataag gacctggcag gcctgaaagc agacgatgcg
     1921 gaaatgaaaa atgccgccag tgttgcagac ctgaaaacgg caattgatgg tgtgtccacc
     1981 agtatgcagg gtgcaggttt cgcactgaaa gattctaagg gacaggaagt gaaacagtct
     2041 cttgggcagg cgattaaagt atccggggac agcaatatca ccaccacagc aatcactgcc
     2101 aatggagata aggggttaaa aatatccctg aataatgacc tgactgtggg ggataaggct
     2161 cccggcagca tcaaagttaa cggggctgat ggtaaggagg ctgtgagtct gaatggtcag
     2221 gatggcacga ttaaactgac atcctcgcct gctgcgggtg gaaagtctgc gtcagcaacc
     2281 attgctgtta atgcaggcaa aggtggatta acagatgctg ccggtaccac gaagacacgt
     2341 ctgacttata ccccggatgg aaaatcaccg gcagaagagc tggcgaccat gaatgacggt
     2401 ctttcttttg agggtgatga cggaaaggtt attcataaaa aactgaatga aaaactgacc
     2461 atcaaaggtg gtgcagagaa gaatgctgaa gtgactgacg gcaatatccg ggttgataat
     2521 gataatggcg ggcttctggt gaggatgacc agaaacctcc gtgatttggc cagcgcggtt
     2581 tttggtacag gtgggcagat aacaacagtt aatgataaag gcatcacaat cacaacacct
     2641 gctgcgggcg gtggtcaggg ggatacggtt tcccttaccg gctccggcct gaataacggt
     2701 ggaaatcaga taaccagtgt ggccagtggt ctgaacggca ctgcgcttgc tgacgccggt
     2761 ggagatgttc tgaataatgc ggccaatatc ggtgacctga aaaatgctct ccgtgacagc
     2821 agtgcggcac tggtgaatac cggctttggt ctgcaggcgg atgacggaag caaggtgaac
     2881 acgtctctcg ggaagcatat cacggttggt ggtgacgggc agaatatcag cacgactgtt
     2941 cagaacggga gcctgacggt cagactcgct gataacattc gcctggcaga tgacggcagt
     3001 gtttctgtcg gcggaacccg gatggataaa aacggactga ccatcaagga tggcccgtca
     3061 gtgactgtga acggtattga tgctgctggc cgggttattt ccggcgtgaa agacggggtg
     3121 aatgaccagg acgccgttaa cgtttcacaa ctgaaccgtg aaatcagtca ggcaacggca
     3181 gcaacgacct gggcactgaa gactgaatcc ggtgatgagg ctgttgctgt ttcctcccag
     3241 acggttgaag tccgtcacgg acagaatacc cgggtttcag ccatcagtaa ggatgataag
     3301 ggaaattaca gctatgagat tgatgtgacg gggattccgg tggagtacac cgacagccag
     3361 gggaacccgc ttgtcaatat tggtggtcgt ttttacacac agaccgaaga cgctgcgacc
     3421 gggaaactga caatgacgcc ggctgagccg gcccgtgtca gaataagcag tgagcagcct
     3481 cttgttctga caaatgtggc ggccggaaat gtgtcaccgg tatccacgga tgcggttaac
     3541 gggagccagc tggcatctgc agccagaatt gctggcggtg ataatgttgt ctggaaagac
     3601 ggaaaagcag cgctggctcc tgacaccttc agtgagctga caactgcagg tggcggtaag
     3661 acaacggtga atggtggtgt acataaaaca ccggataatg tggctgatgc agtgaatatg
     3721 ctgaaccgtg aaggcacgaa gtattttaaa gctgcctctt ccggtcaggc agcaaaagcg
     3781 gaaggacgtg acagcattgc tgtcggacag ggggccgttt cccggggaca aaacagcatt
     3841 gccatgggta atggtgctga agtcacgcgt caggctgctg ccggctctgt tgcagttggc
     3901 agccatgccc gtgctggccg cgcgaatacc gggacttatg ccctgaacgg tcaggctgtt
     3961 gcgggacgta ccggaagtga tactgcggtg gtttcttttg gtcagccggg acaggagcgt
     4021 cagctgcagt cagtggcacc gggtgtgttg tctgcgaatt caacggatgc cgtcaatggc
     4081 agtcagttgc atgcaactaa ccgtcaggtt gccggaaata cacaggcgat aaacactctc
     4141 gggaataaat tcagtcagct gagctaccgg gtggaagagc ttaaccggga tatccgtggt
     4201 gttggtgcat cggctgcggc catgagcggg atacctcagg cctatcttcc gggtaaaagt
     4261 ctgatggggc tgggtgtcgg tggatatggt ggtgaaagcg ccattgcaat aggtgtgtcc
     4321 cgaatctccg ataacggtaa gatgataatg aaactgaatg caggtcagaa cacccggggt
     4381 aatttctccg tcggtgccgg tgtgggctgg cagtggtaac agggaaaagc aaggagaaat
     4441 gtaatgttat ttagtcgaat gatgatgacg ggtgtgctgt ccctgatgct tgcaggatgt
     4501 ggaaatctga gtcatgtcac ggatgaagga acgacggaag aagtggtatg gccttctccg
     4561 gagaaggtga tgtttaatca tgacggaaca cagaaaggtt cgtggccact gccagaaaag
     4621 ttacgtcaga taaaggcggg tatgaataag gaccagattt atcagctggc ggggagacca
     4681 cactttgctg aagggatgtc tggtgtaagg gaatgggatt acctgttcaa ctttaccgga
     4741 ccggaggggg attatacctg ccagtacaag atactgtttg accggaacat ggatgcccgc
     4801 tctttctact ggaagccgga aaactgtctg ccggaggcag agacagcaga atacagggag
     4861 tcagtccccg gtgatatatt atttgatttt gattcatgga ctctgacaga gagggggcgt
     4921 gatgcgcttt cccgtgtggc agagaagttt caggcgggtc atgttgtcgg ggtgaagata
     4981 gaaggattta cggaccatct tggcgggcag gcgtataacc ggctcctgtc agagaaacgg
     5041 gcggaatcag tcagggggtg gtttattgcc cggggattca gggctgaaag gatacatgca
     5101 acgggccgtg gatatgatgc accagtggtg ttctgcccgg atgtggaggg cgaagcactg
     5161 aaggaatgtc tgcgccccaa ccgcagggtt gaaataaccg gggaaacatc agtgactgat
     5221 taacaataaa gtgccccggg agctaatgcc agtcagttca gcaactgact ggctcttttt
     5281 cggggctgtg gggtatttcc agggagcctg tttaggaatc tgtgtaaatg ccttttctca
     5341 gaagtgaccg tccaggcggt caccgaactc gataataaag cggctcattg ccattcgccg
     5401 gttttgccat tctacaacct gctccatcac ggcatcggtg acctttgata tcagtgccgg
     5461 tgaaacatct gcgtcataca gctctttgaa cgcagcagcg atctcacggg tggtcatccc
     5521 tttggcatac aacgagaaga tctggttatc catcccagta atacgggtct gatttttctt
     5581 taccagttgt ggttcgaagg taccgtcacg atcgc
//
LOCUS       DAZKFU020000282         5381 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZKFU020000282 DAZKFU020000000
VERSION     DAZKFU020000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.284"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..292
                     /locus_tag="ACHOQQ_RS21305"
                     /old_locus_tag="pgaptmp_004261"
     CDS             53..292
                     /locus_tag="ACHOQQ_RS21305"
                     /old_locus_tag="pgaptmp_004261"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4472437.1"
                     /translation="MTLDEYLRQSSIRQADFAAIVGVSQGFVSGVITGRYMPQGRKAI
                     EWAKATDWNVTPHELNPHDYPNMEDGIPLHVRQTA"
     gene            339..608
                     /locus_tag="ACHOQQ_RS21310"
                     /old_locus_tag="pgaptmp_004262"
     CDS             339..608
                     /locus_tag="ACHOQQ_RS21310"
                     /old_locus_tag="pgaptmp_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472438.1"
                     /translation="MPDQYCDADRDWIQEQLLQLDPATRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAVRKFPPTP"
     gene            816..1193
                     /locus_tag="ACHOQQ_RS21315"
                     /old_locus_tag="pgaptmp_004263"
     CDS             816..1193
                     /locus_tag="ACHOQQ_RS21315"
                     /old_locus_tag="pgaptmp_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472439.1"
                     /translation="MSAATKSALIRTLSTVPLRTEERYSFLADVVTILESQGMHVARD
                     VTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRPRSVMKLRELARRGTEGFVLLRMP
                     KKLTSYSDAGIEIIPANGKGASC"
     gene            1187..2707
                     /locus_tag="ACHOQQ_RS21320"
                     /old_locus_tag="pgaptmp_004264"
     CDS             1187..2707
                     /locus_tag="ACHOQQ_RS21320"
                     /old_locus_tag="pgaptmp_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU4472440.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDRDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVRNWLENGEDLPTICFCVNVAHANFVTREFLQSGIGAEVMTADTPHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTASGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECPKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYSREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVYNFIKSKTIAYAKGRKKAMTGGQHAD"
     gene            2697..3668
                     /locus_tag="ACHOQQ_RS21325"
                     /old_locus_tag="pgaptmp_004265"
     CDS             2697..3668
                     /locus_tag="ACHOQQ_RS21325"
                     /old_locus_tag="pgaptmp_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU4472441.1"
                     /translation="MQTKKAAVGHWGRIFEYYGMPPVTGVKHYNGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMELLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRKLVMDKFSRLSCLRGTTGEAYLQGRGILQLPTESVRFCDRQIARGREYQAIYAIA
                     TDDKGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDAHGAGLAAAFKCG
                     HKNLMSRNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            3668..4117
                     /locus_tag="ACHOQQ_RS21330"
                     /old_locus_tag="pgaptmp_004266"
     CDS             3668..4117
                     /locus_tag="ACHOQQ_RS21330"
                     /old_locus_tag="pgaptmp_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HGU4472442.1"
                     /translation="MEIEMIKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGRSNSPELNNRLLSFF"
     gene            4125..4688
                     /locus_tag="ACHOQQ_RS21335"
                     /old_locus_tag="pgaptmp_004267"
     CDS             4125..4688
                     /locus_tag="ACHOQQ_RS21335"
                     /old_locus_tag="pgaptmp_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4472443.1"
                     /translation="MQQRKPKKCKVCGTPFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRNLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHNRHRW
                     TVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            4685..4879
                     /locus_tag="ACHOQQ_RS21340"
                     /old_locus_tag="pgaptmp_004268"
     CDS             4685..4879
                     /locus_tag="ACHOQQ_RS21340"
                     /old_locus_tag="pgaptmp_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU4472444.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            4872..5306
                     /locus_tag="ACHOQQ_RS21345"
                     /old_locus_tag="pgaptmp_004269"
     CDS             4872..5306
                     /locus_tag="ACHOQQ_RS21345"
                     /old_locus_tag="pgaptmp_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4472445.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
BASE COUNT         1424 a         1181 c         1521 g         1255 t
ORIGIN      
        1 gacatttaaa tcaggtaagc taatatcgga aaattgaaat gggaggatct ctatgactct
       61 tgacgagtac ttgagacaaa gctctatcag acaagccgat tttgcggcga ttgttggagt
      121 ttctcaggga tttgtcagtg gggttattac agggagatac atgcctcagg ggcggaaagc
      181 cattgaatgg gcaaaagcta cagactggaa tgtaactccc catgaactaa acccgcatga
      241 ctacccaaac atggaagacg gtattccgct tcatgtaagg caaacagcct gataaccctc
      301 catcggaggt gtgagtgaac aggcaaatca aaacggttat gcccgatcag tactgtgatg
      361 ctgatcggga ctggatacag gagcaattgt tacagctaga cccggccacc agggtaaaaa
      421 ttgcagcaga gtacgcgaga gtgtaccagg aagagtggga caaggagcct gtatcgttca
      481 ggaagggcaa cagggcaaga cggagcgcca acacccggtt acgggtgtat gtccagaagt
      541 acgcgagagc cagccgtggc tggatgcttt cgccagtagc ggtacggaag tttcccccga
      601 caccatgatg gcggttagtt gcgtcagaaa taatttcaca tgcaactaat ttcctgaaag
      661 gaagcgagga gggggtaagg gggtggagtc gttggtaagg cctgtatccc ttggcctagg
      721 ccagaaggca ggtacatagg ttaggtagat cactgtataa gcgcgcgata gtttctggtg
      781 aaatgattat cgagctaact attgagtgat aaactatgtc agcagcaacc aaatcggcac
      841 tgatccgtac actgagcact gtaccactga gaacagagga gcgttacagc tttctggctg
      901 atgttgtaac gatccttgag tcacagggaa tgcatgtggc aagagatgtc acagtaagaa
      961 tcgatggcag aaatttccgt gttgatattc tcgcgacagc aaaaactggt gggagtgtgg
     1021 ctattgagat cgatcgtagc tctccgcgac cccgctcagt catgaaattg cgtgaattgg
     1081 cacgtcgtgg cactgaaggc tttgtgttgc ttcgaatgcc aaagaaactg accagttata
     1141 gtgatgctgg gatcgagatc atcccggcga atggcaaggg ggcgtcatgc tgaaaatcac
     1201 cccaaacttt gctcaggagc gcggtttaaa ccagctgcgg caccagtgga aacagcatcg
     1261 cacgtacctg atgtatgcac ccactggctc tggcaaaact ggtcttgcag cattcgtcac
     1321 cgccggaatg gttgaacgtg gtatgcgagt gatgtttgtc tgtccgtaca ccatcctgct
     1381 gaaccagaca gcggaacgtt tcaccgaata cggtttgccg tgggaagaaa tcagttttgt
     1441 atggcgtgat caccctaact atgacccgtc acgactgatt cagattgcca gtgccgacac
     1501 gctgatccgt cgtgagtttc ctgacaacat cgatctgctg atcatcgatg aagcacacat
     1561 gaagcgtcgt gcgttacttg aggttatccg ggacagagac atcagggttc ttggactttc
     1621 cggcaccccg ttcgcagcct ggatggggaa gtactacgaa tgcctgatca agcctacaac
     1681 gattcgtgaa cttattcagc gtggtgatct gagcgattat gagttttttg ccccctcaat
     1741 gcctgatctg gctggcgtaa aaaccagtaa caccgtattt ggacgggatt acaacgagga
     1801 gcagctcgca tccatcatgg ggagttcgga tctggttggc gatatcgttc gcaactggct
     1861 tgagaatggc gaggatttac cgacgatttg cttctgcgtg aacgtggctc atgcgaactt
     1921 tgttacccgt gagtttttac agtctggcat tggcgcagag gtgatgacgg cagatacccc
     1981 ccatgatgaa cggcaggaca tcattcgccg ttttgaagag ggtgcgacca aaataattgt
     2041 caacgttggc gtactggttg cgggatttga cagcgatgtt cgctgcctaa tctacgcccg
     2101 tccgaccaaa tcagaaatcc gctggttgca gtgcatcggg agagcgttac gtactgcgtc
     2161 tggtaaaaaa cgcgcattga ttttcgacca ttccggtacg gttcatcggc tgggcttccc
     2221 cgaggatatc gagtatgacg aacttccggg aaaaaatgac ggaatgaaag cgtctgctgg
     2281 cgggagtgag gttaaggctg agaaacttcc cagggaatgc cccaaatgcc acttcatgaa
     2341 gcctgctggc gtgcatatgt gtcccaaatg tggatttcgt cctcttggtg gtgatgacgt
     2401 ggcgacagat cgcgatcgta agctttcacg cgtcaacaaa gggaaacgcg aatacagccg
     2461 cgaggaaaag cagcgttggt ggagtgagat caaggggtat cagaattacc gcaacgcgac
     2521 gggtaaaccc ctgagtgacg gatggtgtgc tcatacctac aaggagaagt tcggggtttg
     2581 gcccaaagga ttcagtaatg cgccgctgca aacctcagtt gaagtgtaca acttcatcaa
     2641 gtcaaagacc attgcctatg ccaaagggcg caagaaagcc atgacaggag gccagcatgc
     2701 agactaagaa ggcagcggtc gggcattggg ggcgaatttt tgaatattac ggcatgcctc
     2761 ctgttactgg tgtgaaacat tacaacggac cctgcccgat atgtggtgcc agaggtaaat
     2821 ttcgctgtga tgataaagat ggctccggtt cgtggatttg cgtctgtggt cacggggacg
     2881 ggatggagtt gttgcaactt gccacgggta agccctgggt gacgttgtgt gatgaaatcg
     2941 atcggctgat cggaaatacc tggaagaggg agaaagtcag ccagcctgta acagagataa
     3001 gcagaaagcg gaagctggtc atggataagt tctccagact ttcatgtctg cggggcacaa
     3061 caggtgaggc gtacctgcag gggaggggaa tactccagtt accgaccgaa tccgtgcgtt
     3121 tctgtgaccg tcagatcgcc agagggcgcg aatatcaggc aatttacgcc attgcaacag
     3181 atgacaaagg ttctctttgc tatctgcatc gtacgttgct ggatggtgat cgcaaggcga
     3241 atgtagaggc ggctaaaaaa atgaccgcgc tacaggagtt gcctggtttg cagcatgcca
     3301 aatcggtggc aatacgcctg tatccggtgt cgtccactct ggggatagcc gagggtatcg
     3361 aaacggcgct ttcatgccgt caaatattcc gctgcaatgt gtggtcaaca atgaactccg
     3421 gttttatgga gaagtttatt gcgccaccag gcgttaatca cctgattata tttgctgaca
     3481 atgatgcgca cggcgcaggt ctggcggctg cctttaaatg tgggcataag aatctcatga
     3541 gtcgtaatga cgttgagaag gtcagtattc gctggcctga cttgccggat tttaacgaca
     3601 tgcttattca gggatgtgaa gcccgtgaac atgtgctgac gcgcaaattc aaagcggagg
     3661 ctgcctgatg gaaatagaga tgatcaaggc ggctaatggc gtatttgtac cggcgtatga
     3721 gcgcgattta ccccgactgg caaaatttaa aaacggtgag ctgtatacac tggaagcaaa
     3781 acttacccgt aacccatctt ttcaccggaa gatgttcgct tttcttaatt tctgctttca
     3841 gtactggtgc gctgaacatg ctggatacga attctctgat gaagcgacgc agtttgatga
     3901 gtttcgtaaa aatctgacaa ttcttgccgg gttctatgat gtggtcacaa ccataagagg
     3961 cgaggtgaga tatcgggcaa aaagcctgag ttacgcgaat atggatcagg atgagtttga
     4021 acgttgctac aacgcaatga ttaatgccgc gttaaagcat gtgtttgggc gctcaaacag
     4081 ccctgaactg aataaccgcc tgctgtcgtt tttctgaggt gatgatgcag caacgtaaac
     4141 ctaaaaaatg caaagtgtgc ggaaccccgt ttgtgccgtt tcgctcgtat cagaaagttt
     4201 gttgcggtca gtgcgcactt gaactggtca gaaaagaaaa ggccatagcc tctgcaaaag
     4261 aacaggcaga caaacttaaa gcgcgaagaa gggacttaca gccccgcagt tactggatta
     4321 agcaggcaca gcaggccgtg aatgcttata tcagggaacg agaccgtaat ttgccgtgcg
     4381 tttcatgcgg gacattcgat tctgctcagt gggatgcagg tcattaccgt acaacagctg
     4441 cggctcccca actcagattt gatgaacgca atatccacaa gcaatgcgtg gtgtgtaacc
     4501 agtacaaaag cggaaatctc gttccgtatc gcgtcgaact gattaaccgc atcgggcagg
     4561 aagcagtaga cgaaatcgaa tcgaaccata accgccatcg ctggactgtc gaagaatgca
     4621 gggcgatcaa ggcgaagtat cagcagaaac ttaaagacct gcgaaacagc agaagtgagg
     4681 ccgcatgacg ttcacagtaa aaaccattcc tgacatgctc gttgaggcat atggaaatca
     4741 gacagaggta gcccgaatac tgaactgcaa tcgtgccaca gtcagaaaat acattggcga
     4801 taaagaaggg aaaagacacg ctatcgtcaa cggtgttctt atggttcacc gcggatgggg
     4861 taaagatact gatgcgtgat atccggcagg ttcttgagcg ctggggggca tgggcggcaa
     4921 ataactatga ggatgttaca tggtcgccca ttgctgccgg atttaaggga ctgatccccg
     4981 aaaaagtaaa atcacgtcca cagtgctgtg acgatgatgc gatggtgata tgcgggtgca
     5041 tagcccgcct ttaccggaac aatcgcgatc tgcatgactt gctggttgat tactacgtgt
     5101 tgggggagac gttcatggcg ctggcacgga aacatgggtg ctctgacacc tgtataggta
     5161 aacgccttca caaagcggag gggattgttg aaggcatgct gatgatgctg ggagtgaggc
     5221 ttgagatgga tcggtatgtt gagcgtgaat tgccgggagg gagaacctct gtattttatc
     5281 agcgaaaaaa tagtttacga tcgtaaaaat cttcatatca tgataagagt ggttacattg
     5341 ccacgctgct taacccgccg atgcgcgggt tttttgtatc c
//
LOCUS       DAZKFU020000283         5371 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZKFU020000283 DAZKFU020000000
VERSION     DAZKFU020000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.285"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1110
                     /locus_tag="ACHOQQ_RS21350"
                     /old_locus_tag="pgaptmp_004270"
     CDS             <1..1110
                     /locus_tag="ACHOQQ_RS21350"
                     /old_locus_tag="pgaptmp_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGU4472446.1"
                     /translation="FAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRV
                     RVKFNWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIM
                     GRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTE
                     VLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVG
                     SNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNN
                     VTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVN
                     GDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1186..>5371
                     /locus_tag="ACHOQQ_RS21355"
                     /old_locus_tag="pgaptmp_004271"
     CDS             1186..>5371
                     /locus_tag="ACHOQQ_RS21355"
                     /old_locus_tag="pgaptmp_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HGU4472447.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESLWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSSFSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGEL
                     RAVYDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRF
                     EYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYSLHMASGAVTAVTYPDGLRSSREYDEKGRLAAETSRSGETTSYSYDDPASEL
                     PTGIEDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRYGQRTAVHQEEGLSIYSLY
                     DSRGRLTGQRDAQGRETRLEYSAAGDLTVIIAPDGSRSETQYDAWGKAISTTQGGLTR
                     SMEYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGRTQRYHYDLTGKLTQSEDE
                     GLITLWHYDASDRITHRTVNSDPAEQWQYDEHGWLTTLSHTSEGHRVSVHYGYDDKGR
                     LTDERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMKL
                     GGTPLVEYTRDRLHRETVRSFGSMAGSNAAYELTSTYTLTGQLQSQHLNLPQLDRDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRHDEYGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHR
                     LVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTIQTGTTRIQTVYEPGSFTPLIRIETENGEQAKARHRSLAEVLQEDTGVTLPAEL
                     SVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLHLYHCDHRGL
                     PQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDEESGLYYNRNRYYD
                     PLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCNNDILFTGGSGPDN
                     KILNELGPRDGIDGLGSQNMKMYSGLLGGDILKPGILGGLTLGSVTQRPSRTAEEEVQ
                     AQIEYLAYKKRCEQKPDSNLSRCAAAIFQKSRKEDCLRMRQEWDDKWWPGKHADEIKN
                     VKASLKNTLIDVKKRCM"
BASE COUNT         1276 a         1366 c         1818 g          911 t
ORIGIN      
        1 tttgcggtaa tcccggcaga ccggacatgg cggccacagc cgttgctgaa accgctggtg
       61 gacggcccgc agagcgccgt cgtgacggga ccggcaggcg aggaaatctt ctgtgatgaa
      121 catggccgcg tgcgggtgaa atttaactgg gaccgctata acccgtcaaa ccaggaaagt
      181 tcatgctgga tccgtgtggc acaggcgtgg gcaggcaccg gctttggtaa cctggcgata
      241 ccgcgtgtgg gtcaggaggt cattgtggac ttcctcaacg gcgatccgga ccagccgatc
      301 attatggggc gtacctacca ccaggaaaac cgcacccccg gcagcctgcc ggggacgaag
      361 acgcagatga cgatccgctc gaaaacctat aagggcagcg ggtttaatga actgaagttt
      421 gacgatgcga cagggaaaga acaggtctac atccacgcgc agaagaacat gaacaccgag
      481 gtgctgaata accgcaccac tgatgtgata aacaaccatg ccgaaaaaat aggtaacaac
      541 caggcgatca ccgttaccaa taaccagatc cagaacattg gcgttaatca gatacagacg
      601 gttggtgtca accaggtgga aacggtgggc agtaaccaga ttatcaaagt gggatcaaac
      661 caggttgaaa aggtggggat cattcgtgcg ctgacggtgg gtgtggctta ccagacgacg
      721 gtaggcggca ttatgaatac ctcggtggcg ttgttgcagt cctcacaggt agggctgcat
      781 aaatcactga tggtgggaat gggctacagc gtcaatgtgg ggaataacgt caccttctcg
      841 gtgggcaaga caatgaagga aaacaccgga caaacagcag tttattctgc cggtgagcat
      901 cttgaactct gctgtggtaa ggcaaggctg gtgctgacga aggacggaag catatttctc
      961 aatggtacgc acattcatct ggaaggggag tcggatgtga acggtgatgc gccagtgatt
     1021 aactggaact gtggtgccac acaacctgta ccggatgcgc ctgtgccgaa agatttaccc
     1081 cccggaatgc cggatatgcg gcaattttga gtcaggtgca aaaaattaaa acggaactgg
     1141 tgcagtgacc agataaacca tattaaaagc agaaggaatt gattcatggg cggaaaaccg
     1201 gcagcgcggc agggtgatat gacccgcaag ggactggata ttgtgcaggg ttcagcgggg
     1261 gtgctgatag gtgcgccgac gggagtggcc tgttcggtat gtccgggagg gattacctat
     1321 gctaacccgg tgaacccgct gctgggtgcg aaggtgctgc cgggcgagac ggaccttgcg
     1381 ctgcccggcc cgctgccgtt tattctttcc cgcgcctaca gcagctaccg gaccagaaca
     1441 cccgcgccgg tgggggtgtt tggtcccggc tggaaagcgc ccttcgatat ccgcttacag
     1501 atacgcgatg aaggcctgat actcaacgac aacggcggca ggagcattca ctttgagccg
     1561 ctgtttcccg gcgagataag ctacagccgc agcgagtcac tctggctggc gcggggcggg
     1621 gtggcggagc agcacagttc gcagccgcta agcgcactct ggcaggtact gccggaagat
     1681 gttcgcctga gtccgcatgt gtacctggcg acaaacagcc ttcaggggcc gtggtggata
     1741 ctcagctggc cggagcgggt gccgggggcg gacgaggtgc tgccgccgga gccgcccgca
     1801 taccgggtgc tgacgggcgt ggtggacggc ttcgggcgga cgctggcctt tcaccgggcg
     1861 gcggagggtg atgtggcggg cgcggtgacg ggggtgacgg acggcgcggg ccgccgtttt
     1921 cacctggtgc tgaccacaca ggcacagcgg gcggaagtat tccgtaaaca gcgcgccacg
     1981 tctttatctt cccctgccgg tccccgttct gcttcctctt cttccttttc ttcagctttc
     2041 cccgacacgc tgcccgccgg tacagagtac ggtgccgata acggtatccg gctggaggcg
     2101 gtgtggctga cacacgaccc ggcatacccg gatgaacagc cgactgcgcc gctggcgcgc
     2161 tacacgtaca cggccagcgg agaactgcgg gcggtgtatg accgcagcgg gacgcaggtg
     2221 cgcgggtttg cttatgatgc ggagcacgcc gggcggatgg tggcgcacca ttatgcgggg
     2281 aggccggaga gccgctaccg gtatgatgat accggccggg tgacggagca ggtcaacccg
     2341 gaggggctgg actaccgctt tgagtacggg caggaccgtg tgaccatcac ggacagcctg
     2401 aaccggcggg aggtgctgta cacggagggc gagggcggcc tgaagcgggt ggtgaagaag
     2461 gagcatgcgg acgggagcat tacccgcagc gagtatgatg aggcggggag gctgaaggca
     2521 cagacggatg cggcggggcg gcggacggag tacagcctgc atatggcgtc gggtgcggtg
     2581 acggcggtga cgtacccgga cgggcttcgc agcagccggg agtatgatga gaagggaagg
     2641 ctggcggcgg agacctcacg cagcggtgag acgacgagtt acagctatga tgacccggcg
     2701 agtgagctgc cgacagggat agaggatgcg acgggcagta caaaacagat ggcatggagc
     2761 cgttacggtc agctgctggc cttcacggac tgctcggggt acacaacgcg gtatgaatac
     2821 gaccggtacg gtcagaggac ggcggttcac caggaagaag gcctcagtat ttacagtctc
     2881 tatgacagcc gcggcaggct gacagggcag agggatgcgc aggggcggga gacgcggctg
     2941 gagtacagtg ccgcaggcga cctcaccgtt atcattgccc cggacggcag ccgcagtgag
     3001 acacagtatg atgcgtgggg aaaggccatc agcaccacgc agggcgggct gacgcgcagc
     3061 atggagtatg acgctgccgg gcgcatcacc gtgctgacca acgagaacgg cagccagtcc
     3121 acgttccggt atgacccggt ggacaggctg actgaacagc gcggttttga cggccggacg
     3181 caacgttacc actatgacct gaccggaaaa ctcacgcaga gtgaagacga ggggcttatc
     3241 accctctggc actacgatgc gtcggaccgc atcacgcacc ggacggtgaa cagcgacccg
     3301 gcagagcagt ggcagtatga tgagcacggg tggttaacca ccctcagcca taccagtgaa
     3361 ggccaccgcg tgtcggtcca ctacggctat gacgataaag gccgcctgac ggatgaacgg
     3421 cagacggtgg agaacccgga gacgggggag atgctgtggg agcatgagac ggggcatgcg
     3481 tacagcgaac aggggctggc gacccgtcag gagccggacg gtctgccgcc ggtggagtgg
     3541 ctgacgtatg gcagcggtta tcttgcgggg atgaagctgg gcggaacacc gctggtcgag
     3601 tacacgcggg accggctgca ccgtgagacg gtgcgcagct tcggcagcat ggcaggcagt
     3661 aatgcagcat atgaactgac cagcacatac accctcacag gccagctcca gagccagcac
     3721 ctgaacctcc cgcagcttga ccgtgactac gactggaacg acaacggaca gctaatccgc
     3781 atcagcggcc cgcaggagag ccgggagtac cgttacagtg acacgggaag gctgacgggc
     3841 gtccacacca cggcagcgaa cctggatatc gatatcccgt atgcgacgga cccggcagga
     3901 aaccggctgc cggacccgga actgcacccg gacagcacgc tcacggcgtg gccggataac
     3961 cgcatcgcgg aagatgcgca ctatgtctac cgccacgatg aatacggcag gctggcggag
     4021 aagacggacc gcatcccgga aggggtgata cggatgcacg acgagcggac ccaccactac
     4081 cactacgaca gccagcaccg tctggtgttc tacacgcgga tacagcacgg tgagccgcag
     4141 gtggagagcc gctacctcta tgacccgctg ggccgccgga cgggaaaacg ggtgtggcgg
     4201 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggaggt gacatggtac
     4261 gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat ccagacggta
     4321 tacgagccgg ggagcttcac accgctcatc agaatcgaaa cagagaatgg tgaacaggcg
     4381 aaggcgcggc accgtagcct ggcggaggtg ttgcaggagg acacgggtgt gacgctaccg
     4441 gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcggcaggg cagcgtgagt
     4501 gaagaaagcc agcagtggct tgcgcagtgc gggctgacgg cggaacagat ggccgcgcag
     4561 ctggaggcgg aatatatccc ggagcggaaa cttcatcttt accactgcga ccaccgggga
     4621 ctgccgcagg cgctcatcag cccggaaggc gaaacggcgt ggcaggggga gtatgacgag
     4681 tggggaaacc tgctgggcga aaccagcgcg cagcacctgc aacagccgta ccgtctgccg
     4741 ggacagcagt atgatgagga gtcggggctg tattacaacc gcaaccggta ctatgacccg
     4801 ctacagggga ggtatatcac ccaggatcca atagatataa aaggaggatg gaatttatat
     4861 tcttatgcgc ttaatccggt aagttggatt gacccattag gattgacgca gtgcgattcc
     4921 gaagggtgta ataatgatat attatttaca ggaggaagtg gtcccgataa taaaatactt
     4981 aatgaattag gtccgagaga tggcattgac ggtttgggtt cacaaaacat gaaaatgtat
     5041 agtgggctat tagggggcga tattttaaaa ccggggattc tgggcggttt gacgcttggt
     5101 agtgtgacac aacgaccatc gcgaacggct gaggaggagg tgcaagctca aatagaatat
     5161 ttagcatata agaagcgttg tgagcaaaaa cctgatagta atttaagtcg ttgtgctgcc
     5221 gcgatctttc aaaagagcag aaaagaagat tgtttaagaa tgagacaaga atgggatgat
     5281 aaatggtggc ctggcaaaca tgcggatgaa ataaaaaatg tgaaagcatc gttgaaaaac
     5341 acacttattg atgttaaaaa aagatgcatg c
//
LOCUS       DAZKFU020000284         5339 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZKFU020000284 DAZKFU020000000
VERSION     DAZKFU020000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.286"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            182..1516
                     /gene="cadB"
                     /locus_tag="ACHOQQ_RS21360"
                     /old_locus_tag="pgaptmp_004272"
     CDS             182..1516
                     /gene="cadB"
                     /locus_tag="ACHOQQ_RS21360"
                     /old_locus_tag="pgaptmp_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGU4472448.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1596..3743
                     /gene="cadA"
                     /locus_tag="ACHOQQ_RS21365"
                     /old_locus_tag="pgaptmp_004273"
     CDS             1596..3743
                     /gene="cadA"
                     /locus_tag="ACHOQQ_RS21365"
                     /old_locus_tag="pgaptmp_004273"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGU4472449.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3802..5259
                     /gene="dtpC"
                     /locus_tag="ACHOQQ_RS21370"
                     /old_locus_tag="pgaptmp_004274"
     CDS             3802..5259
                     /gene="dtpC"
                     /locus_tag="ACHOQQ_RS21370"
                     /old_locus_tag="pgaptmp_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGU4472450.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT         1262 a         1279 c         1301 g         1497 t
ORIGIN      
        1 tcctgatgtg ttaataaaaa acctcaagtt ctcacttaca gaaacttttg tgttatttca
       61 cctaatcttt aggattaatc cttttttcgt gagtattctt agcgccagtt tggtctggtc
      121 aggaaatagt tatacatcat gacccggact ccaaattcaa aaatgaaatt aggagaagag
      181 catgagttct gccaagaaga tcgggctatt tgcctgtacc ggtgttgttg ccggtaatat
      241 gatggggagc ggtattgcat tattacctgc gaacctagca agtatcggtg gtattgctat
      301 ctggggttgg attatctcta ttattggtgc aatgtcgctg gcgtatgtat atgcccgact
      361 ggcaacaaaa aacccgcaac aaggtggccc aattgcttat gccggagaaa tttcccctgc
      421 atttggtttt cagacaggtg ttctttatta ccatgctaac tggattggta acctggcgat
      481 tggtattacc gctgtatctt atctttccac cttcttccca gtattaaatg atcctgttcc
      541 ggcgggtatc gcctgtattg ctatcgtctg ggtatttacc tttgtaaata tgctcggcgg
      601 tacctgggta agccgtttaa ccactattgg tctggtgctg gttcttattc ctgtggtgat
      661 gactgctatt gttggctggc attggtttga tgcggcaact tatgcagcta actggaatac
      721 tgcggatacc actgatggtc atgcgatcat taaaagtatt ctgctctgcc tgtgggcctt
      781 cgtgggtgtt gaatccgcag cagtaagtac tggtatggtt aaaaacccga aacgtaccgt
      841 tccgctggca accatgctgg gtactggttt agcaggtatt gtttacatcg ctgcgactca
      901 ggtgctttcc ggtatgtatc catcttctgt aatggcagct tccggtgctc cgtttgcaat
      961 cagtgcttca accatcctcg gtaactgggc tgcgccgctg gtttctgcat tcaccgcctt
     1021 tgcgtgcctg acttctctgg gctcctggat gatgttggta ggccaggcag gtgtacgtgc
     1081 cgctaacgac ggtaacttcc cgaaagttta tggtgaagtc gacagcaacg gtattccgaa
     1141 aaaaggtctg ctgctggctg cagtgaaaat gactgccctg atgatcctca tcactctgat
     1201 gaactctgcc ggtggtaaag catctgacct gttcggtgaa ctgaccggta tcgcagtact
     1261 gctgactatg ctgccgtact tctactcttg cgttgacctg attcgttttg aaggcgttaa
     1321 catccgcaac tttgtcagcc tgatctgctc tgtactgggt tgcgtgttct gcttcatcgc
     1381 gctgatgggc gcaagctcct tcgagctggc aggtaccttc atcgtcagcc tgattatcct
     1441 gatgttctac gctcgcaaaa tgcacgagcg ccagagccac tcaatggata accacaccgc
     1501 gtctaacgca cattaattaa aagtattttc cgaggctcct cctttcattt tgtcccatgt
     1561 gttgggaggg gcctttttta cctggagata tgactatgaa cgttattgca atattgaatc
     1621 acatgggggt ttattttaaa gaagaaccca tccgtgaact tcatcgcgcg cttgaacgtc
     1681 tgaacttcca gattgtttac ccgaacgacc gtgacgactt attaaaactg atcgaaaaca
     1741 atgcgcgtct gtgcggcgtt atttttgact gggataaata taatctcgag ctgtgcgaag
     1801 aaattagcaa aatgaacgag aacctgccgt tgtacgcgtt cgctaatacg tattccactc
     1861 tcgatgtaag cctgaatgac ctgcgtttac agattagctt ctttgaatat gcgctgggtg
     1921 ctgctgaaga tattgctaat aagatcaagc agaccactga cgaatatatc aacactattc
     1981 tgcctccgct gactaaagca ctgtttaaat atgttcgtga aggtaaatat actttctgta
     2041 ctcctggtca catgggcggt actgcattcc agaaaagccc ggtaggtagc ctgttctatg
     2101 atttctttgg tccgaacacc atgaaatctg atatttccat ttcagtatct gaactgggtt
     2161 ctctgctgga tcacagtggt ccacacaaag aagcagaaca gtatatcgct cgcgtcttta
     2221 acgcagaccg cagctacatg gtgaccaacg gtacttccac tgcgaacaaa attgttggta
     2281 tgtactctgc tccagcaggc agcaccattc tgattgaccg taactgccac aaatcgctga
     2341 cccacctgat gatgatgagc gatgttacgc caatctattt ccgcccgacc cgtaacgctt
     2401 acggtattct tggtggtatc ccacagagtg aattccagca cgctaccatt gctaagcgcg
     2461 tgaaagaaac accaaacgca acctggccgg tacatgctgt aattaccaac tctacctatg
     2521 atggtctgct gtacaacacc gactttatca agaaaacact ggatgtgaaa tccatccact
     2581 ttgactccgc gtgggtgcct tacaccaact tctcaccgat ttacgaaggt aaatgcggta
     2641 tgagcggtgg ccgtgtagaa gggaaagtga tttacgaaac ccagtccact cacaaactgc
     2701 tggcggcgtt ctctcaggct tccatgatcc acgttaaagg tgacgtaaac gaagaaacct
     2761 ttaacgaagc ctacatgatg cacaccacca cttctccgca ctacggtatc gtggcgtcca
     2821 ctgaaaccgc agcggcgatg atgaaaggca atgctggtaa gcgtctgatc aacggttcca
     2881 ttgaacgtgc gatcaaattc cgtaaagaga tcaaacgtct gagaacggaa tctgatggct
     2941 ggttctttga tgtatggcag ccggatcata tcgataccac tgaatgctgg ccgctgcgtt
     3001 ctgacagcac ctggcacggc ttcaaaaaca tcgataacga gcacatgtat cttgacccga
     3061 tcaaagtcac cctgctgact ccggggatgg aaaaagacgg caccatgagc gactttggta
     3121 ttccggccag catcgtggcg aaatacctcg acgaacatgg catcgttgtt gagaaaaccg
     3181 gtccgtataa cctgctgttc ctgttcagca tcggtatcga taagaccaaa gcactgagcc
     3241 tgctgcgtgc tctgactgac ttcaaacgtg cgttcgacct gaacctgcgt gtgaaaaaca
     3301 tgctgccgtc tctgtatcgt gaagatcctg aattctatga aaacatgcgt attcaggaac
     3361 tggctcagaa tatccacaaa ctgattgttc accacaatct gccggatctg atgtaccgcg
     3421 catttgaagt gctgccgacg atggtaatga ctccgtatgc tgcgttccag aaagagctgc
     3481 acggtatgac cgaagaagtt tacctcgacg aaatggtagg tcgtattaac gccaatatga
     3541 tccttccata tccgccggga gttcctctgg taatgccggg tgaaatgatc accgaagaaa
     3601 gccgtccggt tctggagttc ctgcagatgc tgtgtgaaat cggcgctcac tatccgggct
     3661 ttgaaaccga tattcacggt gcataccgtc aggctgatgg ccgctatacc gttaaggtat
     3721 tgaaagaaga aagcaaaaaa taattagctc gtacaaggga agtggcttgc cacttccctt
     3781 ttttgctcat aaggagaaca catgaaaaca ccctcacagc cgcgcgcgat atactatatc
     3841 gtggcgatcc aaatctggga gtacttcagt ttttacggca tgcgtgcctt actcattctc
     3901 tatctcaccc atcagcttgg ttttgatgat aaccatgcca tcagcctgtt cagcgcatat
     3961 gcttctctgg tttacgttac ccctattctc ggcggctggc ttgccgaccg cctgctcggc
     4021 aaccgcactg cagtgattgc cggcgcgctg ttaatgaccc ttggccatgt ggtgctgggc
     4081 attgatacaa attcaacctt tagcctgtat ctggcgctag caatcattat ttgtggctac
     4141 ggtttattca aatcaaacat cagctgtttg cttggcgagc tctacgacga gaacgatcat
     4201 cgacgtgatg gcggtttttc gctgctgtat gctgcgggca atatcggttc tatcgccgcc
     4261 ccaatcgcct gcggcctggc tgctcagtgg tatggatggc atgttggctt tgcccttgcg
     4321 ggtggcggca tgtttatcgg tttgttgatt ttcttaagcg ggcatcgtca tttccagtcc
     4381 acgcgtagta tggataaaaa agcgctcact agcgtgaaat ttgccttacc agtatggagc
     4441 tggttagtgg tgatgctctg tttagcccca gtatttttta ccctgctgct ggagaacgac
     4501 tggtccggat atttgctggc gatcgtttgc ctcattgccg cacaaatcat tgcccgcatg
     4561 atggtcaaat tccccgaaca ccgccgtgct ctttggcaaa ttgtattgtt gatgtttgtc
     4621 gggacattgt tctgggtact ggcacaacag ggcggcagta ccatcagctt gtttatcgat
     4681 cgctttgtga atcgtcaggc attcaatatt gaagtaccta cagcactatt ccagtcggtg
     4741 aatgcaattg cggtgatgct cgctggggtt gtactggcct ggctggcgtc gccagaaagc
     4801 cgcggcaact caacattgcg cgtctggctg aagtttgcct ttggcttact gctgatggct
     4861 tgtggcttta tgttgttggc atttgatgcc cgacatgcag cggctgacgg tcaagcgtca
     4921 atgggcgtga tgatatccgg gttggcgcta atgggctttg ccgaactctt tattgacccg
     4981 gtggcgattg cgcaaatcac gcgtctgaaa atgtctggcg tattaaccgg tatttatatg
     5041 ctggcaacag gcgcggtcgc caactggctg gcaggcgtcg tggcacagca gacgacagag
     5101 tcgcaaatta gcggtatggc aattgcagct taccagcgat tcttttctca gatgggagag
     5161 tggacgttgg cttgtgtcgc gatcatcgtc gtattggcct ttgctacccg ttttctgttt
     5221 agcacgccga cgaatatgat acaggagagc aacgattaac tgtcgtaaaa acaattggtt
     5281 atgaataaat taactttcag tttataacac tagcaaatct agattaataa gtcatttaa
//
LOCUS       DAZKFU020000285         5263 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZKFU020000285 DAZKFU020000000
VERSION     DAZKFU020000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.287"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            90..1703
                     /gene="mppA"
                     /locus_tag="ACHOQQ_RS21375"
                     /old_locus_tag="pgaptmp_004275"
     CDS             90..1703
                     /gene="mppA"
                     /locus_tag="ACHOQQ_RS21375"
                     /old_locus_tag="pgaptmp_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGU4472451.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKSGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1754..2785)
                     /gene="ynaI"
                     /locus_tag="ACHOQQ_RS21380"
                     /old_locus_tag="pgaptmp_004276"
     CDS             complement(1754..2785)
                     /gene="ynaI"
                     /locus_tag="ACHOQQ_RS21380"
                     /old_locus_tag="pgaptmp_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGU4472452.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            3029..3286
                     /locus_tag="ACHOQQ_RS21385"
                     /old_locus_tag="pgaptmp_004277"
     CDS             3029..3286
                     /locus_tag="ACHOQQ_RS21385"
                     /old_locus_tag="pgaptmp_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGU4472453.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(3336..4286)
                     /gene="uspE"
                     /locus_tag="ACHOQQ_RS21390"
                     /old_locus_tag="pgaptmp_004278"
     CDS             complement(3336..4286)
                     /gene="uspE"
                     /locus_tag="ACHOQQ_RS21390"
                     /old_locus_tag="pgaptmp_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGU4472454.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(4438..5190)
                     /gene="fnr"
                     /locus_tag="ACHOQQ_RS21395"
                     /old_locus_tag="pgaptmp_004279"
     CDS             complement(4438..5190)
                     /gene="fnr"
                     /locus_tag="ACHOQQ_RS21395"
                     /old_locus_tag="pgaptmp_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGU4472455.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT         1433 a         1204 c         1259 g         1367 t
ORIGIN      
        1 tgtcactttt ttgatggaag ttgtttgcat ttctttaagg cgaaacaaat aattacgcat
       61 caattttaat gtcggttaga gggaaactta tgaagcactc tgtttcagtc acgtgttgtg
      121 cgctgttggt cagcagcatt tctctttcgt atgctgcaga agttccgagc ggcacagtac
      181 tggcagagaa gcaggagctg gtgcgccaca ttaaagatga gcctgcgtcg ctggatcccg
      241 ctaaagccgt gggcctgcca gagattcagg tcattcgcga tctgtttgaa ggactggtga
      301 atcagaacga gaaaggggaa attgtccccg gcgttgcgac tcagtggaaa agtaatgaca
      361 accgtatctg gacttttacc ctgcgcgata acgcaaaatg ggcggatggc acaccggtaa
      421 cggcgcaaga ttttgtctac agctggcagc gtctggtgga cccgaaaacg ctatcgccat
      481 ttgcatggtt tgccgcgctg gcgggaatca acaacgcaca ggcgattatt gatggtaaag
      541 ctacgcctga ccagcttggc gtcaccgcag ttgatgccca tactttgaaa attcagcttg
      601 ataaaccgtt gccgtggttt gtgaatttaa ccgctaactt tgccttcttc ccggtgcaaa
      661 aagccaacgt agaaagcggt aaagagtgga cgaaatccgg aaatctgatc ggcaatggcg
      721 cttatgttct taaagaacgc gtagtcaatg aaaaactggt cgtggtaccg aatacccatt
      781 attgggataa cgccaaaacg gtactgcaaa aagtgacctt cctgccaatt aatcaggaat
      841 ccgcagccac taagcgttac ctcgcggggg atattgatat caccgaatcc ttcccgaaaa
      901 atatgtatca gaagctgttg aaggatattc cggggcaggt ttatacgccg ccgcagctcg
      961 ggacctatta ttatgcgttt aacacgcaaa aaggtccgac ggcagatcag cgcgttcgtc
     1021 tggcattaag tatgacgata gatcgccgcc tgatgaccga aaaagtatta gggacgggcg
     1081 aaaagccagc gtggcatttt acaccagatg ttaccgccgg atttacgccg gaaccttcgc
     1141 cgtttgaaca aatgagtcag gaagaactga atgcgcaggc aaaaactttg ttgagcgcag
     1201 ctggttatgg tccgcaaaaa ccgctgaagc tgacgctttt gtataacact tcagaaaacc
     1261 atcaaaaaat tgcgattgct gtagcatcga tgtggaaaaa gaaccttggc gtagatgtta
     1321 aattgcaaaa tcaggaatgg aaaacctata tcgatagccg taacaccggc aattttgatg
     1381 ttatccgcgc ctcatgggtg ggggattata atgaaccctc cactttcctg acattattaa
     1441 cgtcaacgca ttcaggaaat atttcacgct ttaacaatcc ggcatacgac aaagttctgg
     1501 cccaggcatc gacggaaaat accgttaaag cgcgtaatgc cgattacaac gcggcagaaa
     1561 aaatcctcat ggagcaagca ccgattgcac caatttatca atataccaat ggacgattaa
     1621 tcaaaccgtg gctgaaaggt tatcccatta ataaccctga agatgtggcg tacagtcgga
     1681 ctatgtatat tgtgaagcat tgatgtgatg ggaactggcg ttacccttgt ggataacgcc
     1741 agtgatgtct gatttagcga ccctgttccg gcggtgtaat gttatccata tacagcgtct
     1801 ggctggggaa ggcaaagtcc gcgccgtgtg actgtacaat atcgataatc ttcaaataaa
     1861 cgtcttgctg tgcagcaagc cactcagccc ataccgtggt tttggtaaag caataaacca
     1921 taatattcaa tgacgagtca gcaaactggt tgaaataaac cagtaaggtt tgtcgctggt
     1981 cgatggccgg gtgatttttc agcatctcac gtacggcttc gacgataacg cccacttttg
     2041 ccgcatcctc ataacgtaaa ccaatggtcg tggtaatgcg gcggttggtc attcgtcctg
     2101 ggttttctac gctgatcgac gaaaacagcg agttcggtac gtacaatgga cgattatcaa
     2161 aggtcgtaat tttggtaatt cgccagccaa tttccgctac tgtaccttcg atatttctgt
     2221 ccggtgaacg gatccagtcg ccaatactga aaggacggtc gaaatagagc atgatcccgg
     2281 aaaagaaatt actcagaata tctttaccgg ccataccgac agccagacca ccaataccac
     2341 caaaggtcag caagccagaa aggctcatgc cgaaatgttc gccataaagc agaacaagca
     2401 ccacaataat ggtgattttg ataatgcgcg acataatccg cgcactggtg atatcgcgac
     2461 cttttttaat ctgctgtttt tcaaactgat taatcagcaa aaatagctta atcgtcagaa
     2521 taaccgcaat cagggacgta cagataaaat cgataacgcc tggggtgata aatttgagtt
     2581 tatagttttc tataacataa ttaataatgc taccaacagc actgataatt atggtgtaga
     2641 ttaaaaattg caccgcatgg aataaaaagc cttttctttt acgatttccg cggcgaaacc
     2701 aaaagctcat cagaatcaat gctgcgcagc taccgaaaat aatgaccaga ttaagcgcat
     2761 tatttgtaaa cagttcagcg atcattgttt tatcaggctc ctccagataa ttgtcgtcat
     2821 gccggaaacc cctggcgggg ctattttacc gcgacaattc attcagatca tcaatagtca
     2881 gggaaggaag tagcaacatt agctaactaa tcaatgcgtt gattgtaaat ccagctaaga
     2941 ggtgaggttt tcagagcaga caacggtgaa atgtcatggt attgttacgt ttaggtaaca
     3001 agaaatttgt ctgcacaagg attacatcat gattatggcg aaactgaagt cagcgaaagg
     3061 gaagaaattt ctctttggtt tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg
     3121 cgcgactatc ggcggcgtta tagaacagta caatattccg ctgtctgagt ggacgacatc
     3181 aatgtatgtg attcagtcat cgatgatttt tgtttatagc ctggtcttta ctgtgttgct
     3241 ggcaatcccg ttgggaattt atttccttgg cggcgaagag cagtaagtaa aaaataggcc
     3301 cgataactcg ggccttgtca gttattgaag agtcgttaat cgtcttcttc gtcatccagt
     3361 tcaacgggtg tctgatactg gtcaggttta atgaccaaca gatcgcagcg aagatgatca
     3421 atcacctgtt ccgccgtgtt gccgaggaat gctgctgaaa taccggtgcg tcctaccgtg
     3481 cccagaacca caatccccgc ctgtaagtgc tccgccaaat caggaatcac ctcttctggc
     3541 agaccttttt ctacgtgcgt catgttttca ttaatgccga atttctgccg cagagctttc
     3601 attgccagca aatgttgccc acgaatggca tcgttataaa cgctcggatc aaattccggc
     3661 agttcaatcg cgatattaat tggcgttact gggtaagcgc caaccagatg aacttcggta
     3721 tggttgactt gttctgccag ttcgatcgtc tctttgacca gtttttcatt gagcgcatta
     3781 tgatacggct cttcactggc gagattcacc gccaccagcg ccttacctcc ttccggccac
     3841 ggctggtctt tcaccatcca caccgggctt gggcatttgc gtaacagatg ccagtccgtt
     3901 ggcgtaaaaa tcaccgcttc cagacggtca tgttggtgcg ccatttttag caccaaatcg
     3961 tgcccgccgc tgatcacttc ctgaatgatg gcttcgaaag gacggttatg ccagaccact
     4021 ttaatttcaa tgggaacgcc agcattgaga taatattttg cctgctcgtg gatccaggct
     4081 gtacgctggc tgatgacgcc ctgacgcata gcggtacgtt cgtccgggga gagcagggtg
     4141 gtcatttcgt atgagaagtc atagatcggc aaaaaggctt taattttgcc accaatccgt
     4201 tgatgtaaat aaacagctcg ccgcaatgct ggttggtcgt cctggttagg atcgataaca
     4261 acgagcatgt tctgatacat agccatacag ggtctcctta caacaactgt caacgcagtt
     4321 tgtaattaaa agattaaccc atatctggtg aatgaaacag tgatgaacct tctgccagat
     4381 caataaatca gaaaaattta atgatatgac agaaggatag tgagttatgc ggaaaaatca
     4441 ggcaacgtta cgcgtatgac cagcaagctg ggccagcgca tcgttatttt cgatggtgat
     4501 gtatttacct ttgactgcca gcataccgct tttctggaag cgacccagca gacggctgat
     4561 ggtttctacc gtcaggccca gatagttacc gatatcgcca cgagtcatcg tcaggcggaa
     4621 ttcacgaggg gagaagccgc gttgggcaaa acgacgggac aggttgtaga tgaatgcagc
     4681 cagacgttcc tcggcatttt tcttcgacaa cagcaggatc atgtcctgat cgcctttgat
     4741 ttcaccgctc atcagacgca tcatctgctg acgcagattc ggcattttac cggacaaatc
     4801 gtccagcgtt tcgaacggga tttcacatac catcgaggtt tccagcgcct gcgcgaagct
     4861 cgggtgatgg ccgctgccga tggcgtcaaa tcccaccagg tcgcctgcta aatggaaacc
     4921 agtgatttgc tcgtcgcctt gctcagtgat ggtataactt ttaatcgtac cggagcggat
     4981 ggcataaagc gatttaagtt catcaccagc cttaaacagc gtctggcctt tctgaatagg
     5041 cttcttccgc tcaatgatat tatcaagctg atcaagctca tgttcgttga gtgtgaacgg
     5101 gatgcaaagc tggctgatgc tgcaatcctg gcaatggata gcacaaccgc cagactgaat
     5161 gcgccgtata attcgctttt ccgggatcat aggtctgctc aagccgtaat tgatatttgt
     5221 caattttaac atctttttag ggagcaagta agtctaagca aac
//
LOCUS       DAZKFU020000286         5248 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZKFU020000286 DAZKFU020000000
VERSION     DAZKFU020000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.288"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            623..2179
                     /locus_tag="ACHOQQ_RS21400"
                     /old_locus_tag="pgaptmp_004280"
     CDS             623..2179
                     /locus_tag="ACHOQQ_RS21400"
                     /old_locus_tag="pgaptmp_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGU4472456.1"
                     /translation="MHQYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILAT
                     LFWHTPVQDLGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMNILRNVLAAVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLAAFNILLPFVIVAIIGEGLKAIRGVGFMTL
                     MCGVSTLIPQYLVAVYLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIETSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSTVSQIASSLSFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            2229..2948
                     /locus_tag="ACHOQQ_RS21405"
                     /old_locus_tag="pgaptmp_004281"
     CDS             2229..2948
                     /locus_tag="ACHOQQ_RS21405"
                     /old_locus_tag="pgaptmp_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGU4472457.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQNSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            2959..4374
                     /locus_tag="ACHOQQ_RS21410"
                     /old_locus_tag="pgaptmp_004282"
     CDS             2959..4374
                     /locus_tag="ACHOQQ_RS21410"
                     /old_locus_tag="pgaptmp_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGU4472458.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSLAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREEATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNGVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFREVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKELPYACSLCTACNGVCPVKIPLAQLIQKHRQVMAES
                     EITPSTERRITRIFNYVNSHPALWKVGMIAGAKTASLCIRNGKTPISPGAIKEWSVAR
                     DLPSPDGESFRSWFKRHKKGG"
     gene            4379..5074
                     /locus_tag="ACHOQQ_RS21415"
                     /old_locus_tag="pgaptmp_004283"
     CDS             4379..5074
                     /locus_tag="ACHOQQ_RS21415"
                     /old_locus_tag="pgaptmp_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGU4472459.1"
                     /translation="MENREEFLVAIARKLGRPVRRIPEAMPEPVSTLATTRLTELTMD
                     QRCEAFIKFASEVMLAKCVLVSPEDAPSKALDICWTFGSGPVIISNDQRLFDTGIAPL
                     LQKEMGAVLWTPEKGEENIHLAEKAKTGVVFAEYGLTESGGVVLFSAPERGRSVSLLP
                     EASVFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDC"
BASE COUNT         1390 a         1154 c         1278 g         1426 t
ORIGIN      
        1 gtgggagaat tcgctctccg gggcgtctgc gtccccggcg gagaataaac cggagatgat
       61 gattctccac aggagtattc aatggcatac cattccccgg atgtcacact gcattatccg
      121 tatctgcgcc ggatgtcctg acacgtctgc ctgaatggcc ggaagagcgc ttgcacgact
      181 tactaccgtt ctcagcctac cgattcgcag aattaactga cgttacggtc ataccaccta
      241 atgcagggaa acgagtcatg gaggtcgtca gctctactcc catgaatgac acagcctctg
      301 aagtaaaaat accgacgcag tgaaaccagc cttaagcttt ccaaaacaca attacaacat
      361 agttgaacaa agttcattca aacaggttct tttaataaca tttagtcaaa taaaataaca
      421 ataaaacgat tcagcaatca tttttcataa caaaaacaaa ttataacccc cttccctgtt
      481 tgtggacgag actcgttctc tccctgcgag acaagcgtaa attggtcaca ccgacggtag
      541 ttgcctgaaa attacaaatt ctcgtctgac agggcaataa caacagtaat gatgctgaat
      601 ttgttttcgt tgaggaaatg acatgcacca atacctgctt tttttactgg gacttacccc
      661 acttctggca atgatcattt tgattctgaa agtcaaaatg cccatacatt atgccgtact
      721 cagcacactc tttattacac tgattcttgc aactttgttc tggcacacac cagttcagga
      781 tctgggttct gctgttgtat atggcgcaat caaagggctg tggccgatag tgatagtcat
      841 acttgctgcc atttacagct ataacctgat gaatgcgaca aaaagcatga acattctgcg
      901 aaatgttctg gccgctgtca gtgatgataa acgtatccag gtgcttctta tctcatggtg
      961 ttttggtgga tttcttgagg cggcggccgg gtacggtaca gctgtagcaa tccccattgg
     1021 tatactcatt gcactgggct ttaaccctct taaagccgca attgcatcat tggttgctaa
     1081 tacagtacct accgcgtttg gtgctgtcgg catacctgta tcagtacttg ctgaacaggt
     1141 aaatttaccc gtaacaaccc tcagcgggac gattattctt cagctggcag cgttcaacat
     1201 tctgcttccc ttcgttatag tcgctattat tggggaaggc ctgaaggcta tacgaggcgt
     1261 tgggttcatg accttaatgt gcggtgtgag cacattaatc ccacagtacc tggtggctgt
     1321 ttatcttggc gcagaacttc ctgctttcgc gggaagtctg gtcagcctga tcgtgatagt
     1381 ctttatggca aaaaaccgta aagaaaaaac agctcctgaa tttcttattg agacttcagc
     1441 agatagcgcc agcgcacttt catactctcg tggggcgttg ttacgtgcgt gctcaatcta
     1501 catcctgatt tttctgttca ttcttctttg ctctccactg ttcccgacgg taaaaagtac
     1561 agtgtctcag attgcctcgt cactcagctt ctctctgagt aacggcagca atctgtcgat
     1621 gaagattgaa tggatcgcaa cccccggtgt tctgattatc attgccagtg ttattggcgg
     1681 cttgatacag aaagcatccc tgtcatgcct gttaaatgta tttgtcagta cggtaaaaca
     1741 gctcaaaaac tccattattg ccatttctgc gattgttgct atggcaacgg taatggatgt
     1801 ttgtgggttg attggcactc tggcacagag ccttgtagat ctcactggtg gggcctacct
     1861 cttcattgct ccggttatcg gcgctcttgg tacatttgtt actggcagtg acacaaactc
     1921 caacattctg tttggtaaat tgcagaccac tgcggctgaa aaattgcatg tggacccact
     1981 ctggctggct gccgctaaca catctggcgc aacaggcggc aaaatgattt ctcctcagag
     2041 tattgctatc gctgtttcag ccacccgtat ggatggtcag gggagcgtaa tcatgtcagg
     2101 gacactgaag tactgctttg cttatattgt tattcttgga ctgaaagtag gccttgtcta
     2161 ccacctgttc atggcctgag aaaaaataac aaacggacgt ggaatattaa tcaacaggag
     2221 aaatatctgt gaatgttaac tttttcgtca cctgcattgg tgatgcattt aaatcccgta
     2281 tggcacaaaa cagcgtgctg ttactggagc aattgggttg caatgtccat ttccccgaaa
     2341 aacagggatg ttgcggtcag ccagcgataa atggcggtta tgttcgtgat gccattcctg
     2401 gtatgaaaaa tctcatatct tcgctggaag agaatgactg gccggtagta tctcctgccg
     2461 ggtcatgcac ttacacgata aaaaattacg ctcgctatct cagtgaagaa cctgagtggg
     2521 cggcacgagc tgaaaaagta gctgaacgcc tgcatgattt gacgtcattt atagtaaatc
     2581 atcttggcat cacagatgtt ggggcaagac tcaatggtcg cgcagtgtac catccttcat
     2641 gcagcctttc ccgtaaactg ggtgtaaccc gagaaccgct tttgttgctt gagaatgtac
     2701 ggggacttga gctgctgccg tttcacgctc aggaaacgtg ctgcgggttt ggcggcacgt
     2761 tctctgtaaa aatggcggag atctctggtg aaatggtgaa agagaaagtc gggcatatta
     2821 tggatgttaa acctgatttt cttatcgggg ctgacgccag ttgcctgctc aacatcggcg
     2881 gtcggcttag ccgtgaaggg catcctgtaa aagtgatgca tattgctgaa gtcctgatgc
     2941 acagatgagg taaacagaat gtctttgaaa acaagtgata tcaggtttaa agagcgtatc
     3001 cgtattcaga ttcaggaccc gatcatgcga aaagctgtgg ctaacgcaca ggagcgcata
     3061 gggactaatc gacagaaaat ggttgatgaa ctggggcact gggaagaatg gcgttcactt
     3121 gctgaacaga ttcgtcggca tgttctggaa aatcttgatg cttatttgta tcaattgtcc
     3181 gaaaaagtta ctgcgaatgg cggacatgta tattttgccc gtaccagaga ggaggcaacc
     3241 agttacatac tgaaggtggc gaaagaaaaa aacgccagga aggttgttaa atccaaatca
     3301 atggtgactg aggaaattgg tatgaacggc gttcttcaga aagccgggat accagtgata
     3361 gaaacagatc ttggtgaata tattcttcag ctggcgcatg agccgccatc gcatgttgtt
     3421 gtgccggcaa tacataaaga tcgccatcaa attcgcagag ttttgcatga acatctgggg
     3481 tatgacggcc ctgaaactcc agaagcaatg acattgttca tccgtgagaa aatcagacag
     3541 gattttctga gtgctgaagt tggcgtaaca gggtgtaact ttgccgttgc agaaacaggc
     3601 tcagtttgcc ttgtgacaaa cgaaggtaac gcacgtatgt gcaccacgtt acctaaaaca
     3661 catattgctg tgatgggtat ggagcgtatt gcaccaacat tccgggaggt ggatgttctt
     3721 atcaccatgc ttgcaaggag tgctgtggga gcaagactga caggttataa cacctggctt
     3781 acaggtccaa gagaagaagg acatgttgac ggcccggaag agttccatct cgtcattgtt
     3841 gacaatgggc gttcagaggt tctcggctca gaattcagcg atgtattgcg ctgtattcgc
     3901 tgcggtgcct gtattaatac ctgtcccgct tatcgccaga ttggtggaca tggctatggc
     3961 tcaatctacc cgggcccaat cggagcagtt atttccccgt tgcttggtgg atatgaagac
     4021 tttaaggaat taccttatgc ctgctcattg tgcactgcct gcaatggggt atgtccggta
     4081 aaaatacctc tggcacaact aatacaaaaa catcggcagg ttatggcaga gtccgaaata
     4141 acaccatcaa cagaacgccg tataaccaga atattcaatt acgtaaacag tcatccggca
     4201 ttgtggaaag ttggcatgat tgctggcgcg aaaacagcca gcctgtgcat caggaacgga
     4261 aaaacgccga ttagcccagg tgctattaaa gaatggtctg tggccagaga cttaccttct
     4321 cctgatggag aaagcttccg tagttggttc aaaagacata aaaaaggagg gtaactttat
     4381 ggaaaacaga gaggaatttc tcgttgctat cgccagaaag cttggcaggc cagtaagacg
     4441 tattcccgaa gcaatgccgg agcctgtaag tacgctggct acgacacgtt taaccgagtt
     4501 gacaatggac caacgctgtg aggcttttat taaatttgcc tcagaagtga tgcttgcgaa
     4561 gtgcgttctg gtttcgcctg aagacgcacc atcaaaagca ttagacatct gttggacgtt
     4621 cggttccggg ccagtcatta tcagcaacga tcaacgactg tttgatacgg ggattgcacc
     4681 attgctacaa aaagagatgg gcgctgtcct gtggaccccg gagaagggtg aggaaaatat
     4741 tcatcttgca gaaaaagcaa aaacaggcgt cgttttcgct gagtatggac tgacagaatc
     4801 tggtggtgtt gttttattct ccgcccctga acgagggcgt tcagtaagtc ttttacctga
     4861 agcgtctgtg tttgttttac gcaaaagtac cattcttccc agagtggctc agcttgcgca
     4921 aaaacttcat gatatggctc aggaaggaca acgtatgcct tcctgtatca atctgattgg
     4981 aggcccaagt tctactgcag atatagaact gatcaaagtt gttggtgtgc atggacctgt
     5041 acatgcaact tacttgatta ttgatgactg ctaatacatt ttagtatgta tatatgatta
     5101 acagctccgt ttagctgagc tgttaatcat tcatttagtt gtattagtcg tgacttgagc
     5161 gtaagcgtca atacggactc cgttaacgcc ttacaatagc agtcagtgtt catgcctgtt
     5221 tattgaggca agggggagtc tgggcgcc
//
LOCUS       DAZKFU020000287         5212 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZKFU020000287 DAZKFU020000000
VERSION     DAZKFU020000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.289"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..1146
                     /gene="pstS"
                     /locus_tag="ACHOQQ_RS21420"
                     /old_locus_tag="pgaptmp_004284"
     CDS             106..1146
                     /gene="pstS"
                     /locus_tag="ACHOQQ_RS21420"
                     /old_locus_tag="pgaptmp_004284"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGU4472460.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            1233..2192
                     /gene="pstC"
                     /locus_tag="ACHOQQ_RS21425"
                     /old_locus_tag="pgaptmp_004285"
     CDS             1233..2192
                     /gene="pstC"
                     /locus_tag="ACHOQQ_RS21425"
                     /old_locus_tag="pgaptmp_004285"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGU4472461.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2192..3082
                     /gene="pstA"
                     /locus_tag="ACHOQQ_RS21430"
                     /old_locus_tag="pgaptmp_004286"
     CDS             2192..3082
                     /gene="pstA"
                     /locus_tag="ACHOQQ_RS21430"
                     /old_locus_tag="pgaptmp_004286"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGU4472462.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3253..4026
                     /gene="pstB"
                     /locus_tag="ACHOQQ_RS21435"
                     /old_locus_tag="pgaptmp_004287"
     CDS             3253..4026
                     /gene="pstB"
                     /locus_tag="ACHOQQ_RS21435"
                     /old_locus_tag="pgaptmp_004287"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGU4472463.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4041..4766
                     /gene="phoU"
                     /locus_tag="ACHOQQ_RS21440"
                     /old_locus_tag="pgaptmp_004288"
     CDS             4041..4766
                     /gene="phoU"
                     /locus_tag="ACHOQQ_RS21440"
                     /old_locus_tag="pgaptmp_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGU4472464.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         1235 a         1290 c         1394 g         1293 t
ORIGIN      
        1 tgtcatcaaa ctgtcacatt ccttacatat aactgtcacc tgtttgtcct attttgcttc
       61 tcgtagccaa caaacaatgc tttatgaatc ctcccaggag acattatgaa agttatgcgt
      121 accaccgtcg caactgttgt cgccgcgacc ttatcgatga gtgctttctc tgtgtttgca
      181 gaagcaagcc tgacaggtgc aggtgcaacc ttccctgcgc cggtgtatgc caaatgggct
      241 gacacttacc agaaagaaac cggtaataaa gttaattacc agggtatcgg ttcttccggt
      301 ggcgtaaaac agattatcgc caataccgtt gattttggtg cttctgacgc gccgctgtct
      361 gacgaaaaac tggctcagga aggtctgttc cagttcccga ccgtgattgg cggcgtggtg
      421 ctggcggtta acattcctgg gttgaagtct ggcgagctgg tgctggatgg taaaaccctc
      481 ggcgacatct acctcggcaa aatcaagaag tgggatgatg aagccatcgc caaactgaat
      541 ccgggtctga aactgccttc acagaacatt gctgtagtac gccgcgcaga tggctccggg
      601 acttccttcg tcttcaccag ctacctggcg aaagtgaatg aagagtggaa aaacaacgtt
      661 ggtactggct ctaccgtaaa atggccgatc ggtctgggtg gtaaaggtaa cgacggtatc
      721 gctgcgttcg ttcagcgtct gccgggtgca attggttacg ttgaatatgc ttatgcgaag
      781 caaaacaacc tggcgtacac caaactgatc tccgctgatg gtaaacctgt tttgccgact
      841 gaagaaaact tcgctaatgc agcaaaaggt gcagactgga gcaaaacctt cgctcaggat
      901 ctgaccaacc agaaaggcga agatgcgtgg cctattacct ctaccacgtt cattctgatc
      961 cacaaagatc agaagaaacc agaacaaggc acagaagtgc tgaaattctt cgactgggcg
     1021 tacaaaaccg gggctaaaca ggcgaacgac ctggattacg ccagcctgcc ggatagcgta
     1081 gttgaacagg ttcgcgctgc gtggaagacc aatattaaag acagtagcgg caagccgctg
     1141 tactaataaa actccaggcc ggataaggca ttaaacggct tatccggcat tacaaaatga
     1201 ctttgtaaac gcgtttaact gaagagtaac ttatggctgc aaccaagcct gcttttaacc
     1261 caccgggtaa aaagggcgac ataattttca gcgtgctggt aaaactggcg gcgctgattg
     1321 tgctattgat gttgggtggc attattgtct ctctgatcat ctcctcatgg ccgagcattc
     1381 agaaatttgg tctggctttc ctgtggacca aagagtggga tgcgccgaac gatatctacg
     1441 gggcgctggt gccgatctac ggtacgttgg tgacttcgtt tatcgcgctg ctgatcgccg
     1501 tcccggtgag tttcggtatc gccctgttcc tgactgagct tgcgcctggc tggctgaaac
     1561 gcccgctggg tatcgccatt gagctgctgg cagccattcc aagtatcgtt tacggcatgt
     1621 ggggcctgtt tatctttgcg ccgctgttcg ccgtttactt ccaggagccg gtcggcaata
     1681 tcatgtcgaa tatcccgatt gtcggcgcgc tgttctctgg cccggcattt ggtatcggta
     1741 tcctcgcggc aggcgtgatc ctcgccatca tgattattcc gtacattgcg gcggtaatgc
     1801 gcgatgtgtt cgaacaaacc ccggtgatga tgaaagagtc ggcctacggt attggctgca
     1861 ccacctggga ggttatctgg cgtatcgttc ttccgttcac caaaaatggt gttatcggcg
     1921 gcattatgct ggggctgggc cgcgcgcttg gtgaaaccat ggcggtgacc tttatcatcg
     1981 gtaacaccta ccagctcgac agcgcctcgc tgtacatgcc gggcaacagt atcacctctg
     2041 cgctggcgaa cgaatttgcg gaagcggaat ccggtctgca cgttgccgca ctgatggaac
     2101 tgggcctgat cctgtttgtg attaccttca tcgtcctcgc cgcatcgaag tttatgatta
     2161 tgcgcctggc taagaatgag ggggcacgct aatggctatg gttgaaatgc aaaccactgc
     2221 ggcgcttgct gaatcccgcc gcaaaatgca ggcgcgtcgc cgcctcaaga accgtattgc
     2281 actgacgctc tcaatggcga cgatggcctt cggtctgttc tggctgatct ggattttaat
     2341 gtccaccatc actcgcggta tcgatggtat gtcgctggct ctgttcactg aaatgacgcc
     2401 gccgcccaat acggaaggtg gtggtctggc gaacgctctg gcgggtagcg ggctgttaat
     2461 tttgtgggcc acggtattcg gtacgccgct gggcattatg gcggggattt atctggctga
     2521 atatggtcgt aaatcctggc tggcagaagt gattcgcttc attaacgaca ttctgctctc
     2581 tgcgccgtcg attgtggttg gtctgtttgt ttacaccatc gtggtggcgc agatggagca
     2641 cttctccggt tgggcgggtg tgattgcgct ggcgctgttg caggtaccga ttgttatccg
     2701 caccaccgag aacatgctga aactggtgcc ggacagcctg cgtgaagcgg cttatgcact
     2761 gggtacgccg aagtggaaga tgatctccgc gattacgctg aaagcgtcgg tgtccggaat
     2821 tatgaccggt atcctgctgg cgattgcccg tattgccggt gaaaccgcgc cgctgctgtt
     2881 taccgcgctc tccaaccagt tctggagcac ggacatgatg cagccgatcg ccaacctgcc
     2941 ggtgacgatc tttaagtttg cgatgagccc gtttgcggaa tggcagcaac tggcctgggc
     3001 cggggtattg atcattaccc tgtgcgtact gctgctgaac attctggcgc gcgttgtttt
     3061 cgcgaagaat aaacacggtt gatattgctg acacggtttt cccctctccc cagaggggcg
     3121 aggggaccga ccgagcgcct ttttgactct gtacacggtt aacactttgc cggatgcggc
     3181 gtaaacgcct gatccggcct acggtaagcc tgattagcga agtgcatcag gcacgatgag
     3241 gaaaagattg caatgagtat ggttgaaact gccccgagta aaattcaggt tcgtaatttg
     3301 aacttctact acggcaaatt ccatgccctg aaaaacatca acctggatat cgctaaaaac
     3361 caggtaacgg cgtttatcgg gccgtccggc tgcggtaaat cgacgctgct gcgtaccttc
     3421 aacaaaatgt ttgaactgta tccggaacag cgtgcggaag gtgaaattct gcttgatggc
     3481 gacaacatcc tgaccaactc tcaggatatc gccctgctgc gtgcgaaagt gggcatggtg
     3541 ttccagaagc ctacgccgtt cccgatgtcc atttacgaca acatcgcctt tggtgtccgt
     3601 ctgtttgaga agctctcccg tgccgacatg gacgagcgcg tacagtgggc attgaccaaa
     3661 gccgcattgt ggaacgaaac caaagataaa ttgcaccaga gcggttactc tctctctggt
     3721 ggtcagcaac aacgtctgtg cattgcgcgt ggtatcgcca ttcgcccgga agtgctgctg
     3781 ctcgacgaac cgtgttcggc gctcgaccct atctctaccg ggcgtattga agagctgatc
     3841 accgaactga agcaggatta caccgtggtg atcgtcaccc acaacatgca gcaggctgcg
     3901 cgttgttccg accacacggc gtttatgtac ctgggcgaat tgattgagtt cagcaacacg
     3961 gacgatctgt tcaccaagcc agcgaagaaa caaacagaag actacatcac cggtcgttac
     4021 ggttgattca ggagtgcgtt atggacagtc tcaatcttaa taaacatatt tccggccagt
     4081 tcaacgccga actggaaagt atccgcacgc aggtgatgac catgggcggc atggtggagc
     4141 agcagctttc tgatgcaatc accgcgatgc acaaccagga cagcgatctg gcgaagcgcg
     4201 tcatcgaagg cgacaagaac gtcaacatga tggaagtggc gatcgatgaa gcctgcgtgc
     4261 gcattattgc caaacgtcag ccgacagcga gcgacctgcg tctggttatg gtgatcagta
     4321 aaaccattgc tgaactggag cgtattggcg acgtggcgga caaaatctgc cgtactgcgc
     4381 tggagaaatt ctcccagcag catcagccgt tgctggtaag tctggagtcg ctgggccgtc
     4441 ataccatcca gatgctgcac gacgtgctgg acgcgttcgc gcggatggac attgacgaag
     4501 cggtacgtat ttatcgtgaa gataaaaaag tcgatcagga gtatgaaggt attgttcgtc
     4561 aactgatgac ctacatgatg gaagattcgc gtaccattcc gagcgtactt accgcgctat
     4621 tctgtgcgcg ttctatcgaa cgtattggcg accgctgcca gaatatttgt gagtttatct
     4681 tctactacgt gaaggggcag gatttccgtc acgtcggtgg tgatgagctg gataaactgc
     4741 tggcgggaaa agaccccaaa gagtagtgct aactgtaggc cagataaagc tttttgccgc
     4801 atctggcaat cagcccggtc atacatagtt accgggctga taaactttct cactgaaata
     4861 ttgccacaca aactcacctc gaaactctcc ccagtacatt gcatatacat tattacattt
     4921 gaattatttt acttttaatg tagtgccacg ctataaaaac atttttgtta ttcttttcat
     4981 ttatatcgct acctgcacag agaatggaaa agaaaacaaa aaacagggtg aaagccctat
     5041 atcgaaacat acataatgag cgatttttta cagtattcat aggtggttta gccataatcg
     5101 tctcaaacca acacccaggt atgcttttta agacaaatcg gctatgtgtt gtgcttgggc
     5161 gaataaatat tgtgttcagg agaaatgatt atggctatat catcatatag ta
//
LOCUS       DAZKFU020000288         5205 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZKFU020000288 DAZKFU020000000
VERSION     DAZKFU020000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.290"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(161..301)
                     /gene="yjjY"
                     /locus_tag="ACHOQQ_RS21445"
                     /old_locus_tag="pgaptmp_004289"
     CDS             complement(161..301)
                     /gene="yjjY"
                     /locus_tag="ACHOQQ_RS21445"
                     /old_locus_tag="pgaptmp_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGU4472465.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            397..1113
                     /gene="arcA"
                     /locus_tag="ACHOQQ_RS21450"
                     /old_locus_tag="pgaptmp_004290"
     CDS             397..1113
                     /gene="arcA"
                     /locus_tag="ACHOQQ_RS21450"
                     /old_locus_tag="pgaptmp_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGU4472466.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1173..2525)
                     /gene="creD"
                     /locus_tag="ACHOQQ_RS21455"
                     /old_locus_tag="pgaptmp_004291"
     CDS             complement(1173..2525)
                     /gene="creD"
                     /locus_tag="ACHOQQ_RS21455"
                     /old_locus_tag="pgaptmp_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGU4472467.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLGELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKDVTTDDELRIWK
                     "
     gene            complement(2583..4007)
                     /gene="creC"
                     /locus_tag="ACHOQQ_RS21460"
                     /old_locus_tag="pgaptmp_004292"
     CDS             complement(2583..4007)
                     /gene="creC"
                     /locus_tag="ACHOQQ_RS21460"
                     /old_locus_tag="pgaptmp_004292"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGU4472468.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDHEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(4007..4696)
                     /gene="creB"
                     /locus_tag="ACHOQQ_RS21465"
                     /old_locus_tag="pgaptmp_004293"
     CDS             complement(4007..4696)
                     /gene="creB"
                     /locus_tag="ACHOQQ_RS21465"
                     /old_locus_tag="pgaptmp_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGU4472469.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4709..5182)
                     /gene="creA"
                     /locus_tag="ACHOQQ_RS21470"
                     /old_locus_tag="pgaptmp_004294"
     CDS             complement(4709..5182)
                     /gene="creA"
                     /locus_tag="ACHOQQ_RS21470"
                     /old_locus_tag="pgaptmp_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGU4472470.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
BASE COUNT         1329 a         1428 c         1228 g         1220 t
ORIGIN      
        1 ttattagttg tatgatgcaa ctagttggat tattaaaata atgtgacgaa agctagcatt
       61 tagatacgat gatttcatca aactgttaac gtgctacaat tgaacttgat atatgtcaac
      121 gaagcgtagt tttattgggt gtccggcccc tcttagcctg ttatgttgct gttaaaatgg
      181 ttaggatgac agccgttttt gacactgtcg ggtcctgagg gaaagtaccc acgaccaagc
      241 taatgatgtt gttgacgttg atggaaagtg catcaagaac gcaattacgt actttagtca
      301 tgttacgccg atcatgttaa tttgcagcat gcatcaggca ggtcagggac ttttgtactt
      361 cctgtttcga tttagttggc aatttaggta gcaaacatgc agaccccgca cattcttatc
      421 gttgaagacg agttggtaac acgcaacacg ttgaaaagta ttttcgaagc ggaaggctat
      481 gatgttttcg aagcgacaga tggcgcggaa atgcatcaga tcctctctga atatgacatc
      541 aacctggtga tcatggatat caatctgccg ggtaagaacg gtcttctgtt agcgcgtgaa
      601 ctgcgcgagc aggcgaatgt tgcgttgatg ttcctgactg gccgtgacaa cgaagtcgat
      661 aaaattctcg gcctcgaaat cggtgcagat gactacatca ccaaaccgtt caacccgcgt
      721 gaactgacga ttcgtgcacg caacctgctg tcccgtacca tgaatctggg tactgtcagc
      781 gaagaacgtc gtagcgttga aagctacaag ttcaatggtt gggaactgga catcaacagc
      841 cgttcattga tcggccctga tggcgagcag tacaagctgc cgcgcagcga gttccgcgcc
      901 atgcttcact tctgtgaaaa cccaggcaaa attcagtccc gtgctgaact gctgaagaaa
      961 atgaccggcc gtgagctgaa accgcacgac cgtactgtag acgtgacgat ccgccgtatt
     1021 cgtaaacatt tcgaatctac gccggatacg ccggaaatca tcgccaccat tcacggtgaa
     1081 ggttatcgct tctgcggtga tctggaagat taatcggctt taccaccgtc aaaaaaaacg
     1141 gcgcttttta gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc
     1201 gttgtaacgt ctttactggc tttcattttc ggcagtgaaa acgcatacca gtcgatatta
     1261 cgagtcacaa acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc
     1321 gcgctatcgg cagagttgag cagtccccac atcacaccat ccagcaacaa cagcgcgagg
     1381 gtaaacaaca tgctgttgcg ccagcctttc aataccgcct gcaaataaat accgttcatt
     1441 aacgccccaa tcagactggc gattatccat gccacggtaa aaccggtatg ttcagaaagc
     1501 gccagcaaga gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg
     1561 tgtaagcgtt gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagcgcaatc
     1621 agcagaatgg cgtacttagt cgcccggtcc gttaattggt attgatcggc aggcgtcgtc
     1681 actgcgacgc taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc
     1741 tcaccgagat tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta
     1801 acttcccgtt tggctggcaa aaaatcacct aaaaaactgg gatgcggcca gttgctggtt
     1861 aaggtcattt cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa
     1921 ttcagggcca tattcagctt caggttctgc ttgcgccagt ccccttcggg taaagggata
     1981 tgcacgccct gcccgccttg ctctaacccg gtgccgggtt caatggtcag cgccgttccg
     2041 ttaacttcag gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata
     2101 aatggcttgc caaaggtgat atttggcgcg tcgagctcgc caagacgcga aacatcgaaa
     2161 tcggctttta gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt
     2221 tcttccacgt tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa
     2281 cttcgtttcc gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga
     2341 atagcgataa gcggcccaac gagtttttgc ggcccactgg tactttggcg aatcgcatct
     2401 tccacatcgc tacggtaatc ggcacgttcg acaatcacct gccgaatcag cattatcgga
     2461 atcaacaaca gcaatactgc accaaacagg gtggtcattt tccagaacag gggggatttc
     2521 aacatagtct tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga
     2581 agctatgtga agtgacggtg aagtcgaagc gaggccagca cgccgccttc ctgcacgttg
     2641 cgcagcgtga cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga
     2701 ccgctgcttt tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac
     2761 agcgcgtagt caggaatacc actaccggta tccagcacct taagcgtgac gtgttcatga
     2821 tccacttcgg cgcttagcgt gatgcaaccg ctctcgggag taaaatcgat ggcgttatcc
     2881 agtaaattcc ccagcgcctg ctccagtaac gccggttcag cagcaacgtt aacctcagta
     2941 ggcataacat gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg
     3001 cggcgaaata atgccgccac atcaacagca gtcagaacta cttcctgacg attctccagt
     3061 cttgcctggc gtagtaacgt ttctaccaat gcctgcatac gcgcattttg ggtcagaatg
     3121 ttgtcggtaa aacgagccac cacttccggt ggcggacctt cgcgtaaaat ttccgccgcg
     3181 ccacgaatcg ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca
     3241 atatagtttt tcccttccag cttcacgcgc atactttcca gcgcctgcgc aagcttacgc
     3301 agctcgctac tgccgagttc aggaagagga acgggcttat tgtcagtgac ggaatcggca
     3361 tagcgagtga gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc
     3421 agtgcaatcc ccagcaaaat ggcgctggcc cataaaattc gccgctcgct gcgcttaatg
     3481 accggagcca tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg
     3541 tccataatcg gcgcggcaac atacatcact gaactttcgg gatcggcagg attttgcaac
     3601 gtgctgcgcg caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa
     3661 tcctgcccaa cggctttatt tgccgaatcg aacaatactt tgccctgcgc atcggtcata
     3721 tagacatgat attcattgcg cactttgtta atgccaccga tattggcgcg aaacgggcga
     3781 tgttgtagct gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa
     3841 tcgggacgcg ccagctccgc cagcaacgtt gcggtgtcga tcagcgtccc ctccgttgct
     3901 cttcgcacgc ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc
     3961 gccaccagta aaaaatagcc cagcaacaac cgcatgccga tacgcattac aggcccctca
     4021 ggctatatcc catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca
     4081 gcttggcacg caacgtttta atgtgggtat cgacggtgcg atcgtaggtg tcctgcgcat
     4141 cttcccacac gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta
     4201 acgtcttcag caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga
     4261 tctgcgccgc gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgacg
     4321 agaacttctt cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa
     4381 acggtttagc cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt
     4441 cacttcgggc cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc
     4501 acaattcaaa gccgctgata tcaggcaaac caacatcgag aatcatgaca tcgggaacct
     4561 gctggcgagc tttatccagc accggcaagc ctcgctcaaa gacctcgacg gcaaaacctt
     4621 cctgctgcaa catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga
     4681 ccgtttcccg ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc
     4741 taatcgcgtt tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg
     4801 cgttgcgttt ggcatcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc
     4861 gttttttgaa tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa
     4921 tcggcccgac ttgctgacaa gaaatggccg catcggaggt atcttccgcc aggcccaatc
     4981 cccctttaat acccccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg
     5041 gatcgtcaaa ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt
     5101 cgaccgaacc aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca
     5161 ggatcaaatg cttgtatttc atattgttac catttttatc tatta
//
LOCUS       DAZKFU020000289         5157 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZKFU020000289 DAZKFU020000000
VERSION     DAZKFU020000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.291"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..211
                     /locus_tag="ACHOQQ_RS21475"
                     /old_locus_tag="pgaptmp_004295"
     CDS             <1..211
                     /locus_tag="ACHOQQ_RS21475"
                     /old_locus_tag="pgaptmp_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HGU4472471.1"
                     /translation="NFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDGQLQL
                     NVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            238..1071
                     /gene="csgG"
                     /locus_tag="ACHOQQ_RS21480"
                     /old_locus_tag="pgaptmp_004296"
     CDS             238..1071
                     /gene="csgG"
                     /locus_tag="ACHOQQ_RS21480"
                     /old_locus_tag="pgaptmp_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGU4472472.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(1135..1626)
                     /gene="ycdZ"
                     /locus_tag="ACHOQQ_RS21485"
                     /old_locus_tag="pgaptmp_004297"
     CDS             complement(1135..1626)
                     /gene="ycdZ"
                     /locus_tag="ACHOQQ_RS21485"
                     /old_locus_tag="pgaptmp_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4472473.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALTPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1728..2282)
                     /gene="ycdY"
                     /locus_tag="ACHOQQ_RS21490"
                     /old_locus_tag="pgaptmp_004298"
     CDS             complement(1728..2282)
                     /gene="ycdY"
                     /locus_tag="ACHOQQ_RS21490"
                     /old_locus_tag="pgaptmp_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGU4472474.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(2306..3043)
                     /gene="ycdX"
                     /locus_tag="ACHOQQ_RS21495"
                     /old_locus_tag="pgaptmp_004299"
     CDS             complement(2306..3043)
                     /gene="ycdX"
                     /locus_tag="ACHOQQ_RS21495"
                     /old_locus_tag="pgaptmp_004299"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGU4472475.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRALAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3098..4036)
                     /gene="ghrA"
                     /locus_tag="ACHOQQ_RS21500"
                     /old_locus_tag="pgaptmp_004300"
     CDS             complement(3098..4036)
                     /gene="ghrA"
                     /locus_tag="ACHOQQ_RS21500"
                     /old_locus_tag="pgaptmp_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGU4472476.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            4270..4357
                     /locus_tag="ACHOQQ_RS21505"
                     /old_locus_tag="pgaptmp_004301"
     tRNA            4270..4357
                     /locus_tag="ACHOQQ_RS21505"
                     /old_locus_tag="pgaptmp_004301"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4304..4306,aa:Ser,seq:gga)
     gene            complement(4507..>5157)
                     /locus_tag="ACHOQQ_RS21510"
                     /old_locus_tag="pgaptmp_004302"
     CDS             complement(4507..>5157)
                     /locus_tag="ACHOQQ_RS21510"
                     /old_locus_tag="pgaptmp_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4472477.1"
                     /translation="MKQDFRCGCWLMDKPDTAMKAITRNLDREIWRDLMQCSGMLSLM
                     DAQARDTWYRSLEYDNFPEISEENILSTFEQLHQNKDEVFERGVINLFRGLSWNYKTN
                     CPCKFGNKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDNRENITIH
                     LDDHIRSLQGKECYEDEMFIIKYFKKGSAHITFRKPELVDRLNDIIAKHYPDMLAV"
BASE COUNT         1337 a         1338 c         1199 g         1283 t
ORIGIN      
        1 taactttact caggccatcc agtcacaaat tttgggtggg ctactgtcga atattaatac
       61 cggcaaaccg ggccgcatgg tgaccaacga ttatattgtc gatattgcca accgcgatgg
      121 tcaattgcag ttgaacgtga cagatcgtaa aaccggacaa acctcgacta tccaggtttc
      181 gggtttacaa aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg
      241 cagcgcttat ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct
      301 aaagaagccg ccagaccgac attaatgcct cgagctcaga gctacaaaga tttgacccat
      361 ctgccagcgc cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg
      421 caatttaaac cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccacggca
      481 atgctggtca cggcattgaa agattctcgc tggtttatac cgctggagcg ccagggctta
      541 caaaacctgc ttaacgagcg caagattatt cgtgcggcac aagaaaacgg cacggttgcc
      601 attaataacc gaatcccgct gcaatcttta acggcggcaa atatcatggt tgaaggttcg
      661 attatcggtt atgaaagcaa cgtcaaatct ggcggggttg gggcaagata ttttgggatc
      721 ggtgccgaca cgcaatacca gctcgatcag attgccgtga acctgcgcgt cgtcaatgtg
      781 agtaccggtg agatcctctc ctcggtgaac actagtaaga cgatactttc ctatgaagtt
      841 caggccgggg ttttccgctt tattgactac cagcgcttgc tggaagggga agtgggttac
      901 acctcgaacg aacccgttat gctgtgcctg atgtcggcta tcgaaacagg ggtcattttc
      961 ctgattaatg atggtattga ccgtggtctg tgggatttgc agaacaaagc agaacggcag
     1021 aatgacattc tggtgaaata ccgccatatg tcggttccac cggaatcctg acagataaaa
     1081 aagccggaat tatcatccgg cattttactg cttaagcgaa agcgtgagtg cccctcacgc
     1141 tttatttttg atttcctgct cacggtgcgc cgtctttgca ctacgcgccg ccagccacag
     1201 gccgctgttt ttcattgcgt aaccaaatat cagcccaagc gccagcgaag gtaacaccag
     1261 tttccagtca ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa
     1321 tgaaagcagc agctgtttgg cctgaataca catcagaaac gcgacaatac cggttatgac
     1381 ataaccgaga atttccaaat gtggcgtcag tgcactaccg taaataatga ccatcgccca
     1441 caccacgcca ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc caccttgcgg
     1501 gcaggcaaag taggccgtac agcccaggaa gcccgcccag cttagtaagc ccagggaaac
     1561 agccacccat ccccagatac cggagagaat gcctgttgtg attgcaatag agagaagtat
     1621 gttcatgccg cgcatgatag cagaaaacgc ggccatgaat gagatcgagc acacatttta
     1681 tgcaacagca aattatctgt tgcatttatg agtgacgcag atcacattta ctcttcagaa
     1741 tcttcttcca gctcgtccca cattgcacta atggcatcgc gggttagcgg tgccatggtg
     1801 cgccagaaag gcgtggttgc atgggcctcc actttgccaa ggaacgcacc acaccagggt
     1861 aacagatact cactgaacag tgtttccagt gcttcgctct catcttccgt tgactgatct
     1921 tccagccagg aagccgcaag cagcaatgtg ccgatgtgat cggcaggcgt atccgctaat
     1981 ggcatccctc gttcagaaag aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat
     2041 gcgctacgat atggcggcac ggcacattca tcgccgataa acaacgcatt gtaatcggca
     2101 gagacttgcg tcatatcaca gcttttctgc aaacgtgtca gcaactcatc ctgctccagt
     2161 ggccagttcg cagccagttt cccctcacga atcagggtaa acagcggcac cagtaaagga
     2221 tcttgcggtt ggcggtaata gagcgaaccc agcacacgac agaggataga aaactcgttc
     2281 attaatatat ttccattact agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac
     2341 cgcgagattc aaggaagttc agtaagcggc gcggagaaac attcaaaatg cgctctggcg
     2401 gaaaatctac cgcgtcgagg attttaagac actcttcaaa ttcccccatg gtaaacgcgg
     2461 tgtgagaatc cgagcctaat gccacccaac ctccagcatc acgtaccgct gcggctagcg
     2521 cacgacagtt gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta ttgatttcca
     2581 gcgccacctg atgtttcgcg gctgcttcag caacggcttt aacatctatc tcatatttcg
     2641 gatttccggg atggcttatt atatgcacat tgccgctggc gatagtggcg atcatcgctt
     2701 gtgtattggt cgctttatca tgtggcgcaa aaaccggctc atgaaaaccg gcaataatca
     2761 gatccagcga atcaaacatt ttgccgctgc agtcaatttc accatcaaca tttttaatgt
     2821 tagcttcgat gccgcgcagg atccctaccc catcaaccac tcgcggccag atacgcatgt
     2881 taatgaagtg ccagtgatgt ggcgcatctt ccatatccgg gccatgatcg gtgatcgcaa
     2941 aaagtttaat gcccttttgt ttggcctggg caatataatc actcaatgtg ctatatgcat
     3001 gcgtgctggc aacggtatgc atatgaaggt cgacgggata cataactctc tcctgtattc
     3061 aattcccgcc aaggatagca ggaatcctga tgctttatta gtagccgcgt gcgcggtcga
     3121 cttgcccgca gaccctctcc cctttttcga gctgggcaat ggtgcgagaa atgtactcca
     3181 cagcttcagc gggacgggta atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt
     3241 gccagagcgg actttcaggc ggtaagggtt cacgattaaa aacatccagc attgcgcctt
     3301 taactttccc gctatccagc gccgcgagca ggtcatcttc cacaacatga acaccacgcg
     3361 ccaggttgag gagatacgcg ccatccggta atttttcgag taattgttga ttaataatgc
     3421 cgacggtttc aggggtattc ggtaacaaat taatcaatac ccgacattgg ctcagaaatg
     3481 cagacagttc ttcccgtccg gcaaagcttt gcacgccagg ccacgattta cgggttcgac
     3541 tccagcaacg cagcggaaag cgccaggttt gcagactctg agcaacttta ctgcccagta
     3601 cgcctgcgcc caaaatgccg atggtaaaat cttcccgatg atattcaggc agcggttgcc
     3661 aatgcgaact attttgctgg atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct
     3721 gactgacagc atattcctgc atttgctcgc ccataccggt atcttccagg cgaaaaagtg
     3781 gaacagaagg gttcagcatt tcagggtgtg cctgtagctt gctcaaaata gaatcaacac
     3841 cggccccgag tgcgaacacc gctttaagat cgcgccctgc cagcatttca acaggaggat
     3901 gccagactaa agcataatca gcagagtcat tatctccgct tttccatgct ctgactcttg
     3961 cctgaggaat agctttgcgc agtgcctcaa tccaccattg ggtatcgaac gttgggtgat
     4021 aaaagatgat atccatactg actcccgaaa agcgttgtgc gaaatttatt cgcacttatc
     4081 gttatgatct acaaagacca ccagcataac aaatccgtgg tcggtggcaa aaaaagcaga
     4141 tttcgcttat taaaaccaca tattgattga agtttgaata aacgcgcgat tttttaaaaa
     4201 agtttgttga cctcaggtca tgatttccct aaattagcgc ccgttccagc aagacaggaa
     4261 cgacaatttg gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg
     4321 caacgtatcg ggggttcgaa tccccccctc accgccagat tacaagcgca tgttattgtt
     4381 taacatgcgc ttttttattt atccttccag acagtatgcc accgggtatg ccatctgaaa
     4441 ccggtttata tgccccacga tcatatctta tcggacaatt tccgatcttt gagcatactg
     4501 gcaaagctat actgccagca tgtcaggata gtgttttgcg ataatatcat tcagtctgtc
     4561 aaccagttct ggcttcctga acgtgatgtg cgcagagccc ttcttaaagt atttaataat
     4621 gaacatctca tcttcatagc attcttttcc ctgaagagac cggatgtgat cgtccagatg
     4681 aatagttatg ttttccctgt tgtcggggat cggtttgccg ctgaacagat gcagcattct
     4741 ttccaggtcg gcaagtctat ctgcctgttg cccggtgata agatgcaatc cccaccggtc
     4801 ccacctcacc agattgttga cgataatttt attgccaaat ttacaggggc aattggtttt
     4861 gtaattccag ctcaaccctc tgaagaggtt gatcactcct cgctcaaaca cttcatcctt
     4921 attctggtgt agctgctcaa acgtactcag aatattctct tcactgattt ccggaaaatt
     4981 atcgtactcc agtgaccggt accatgtatc acgggcctgt gcatccatta aggaaagcat
     5041 cccggagcat tgcatcaggt cacgccagat ttcgcgatcg agattgcggg tgatagcttt
     5101 cattgctgta tcaggtttat ccatgagcca gcagccgcag cgaaaatcct gtttcat
//
LOCUS       DAZKFU020000290         5149 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZKFU020000290 DAZKFU020000000
VERSION     DAZKFU020000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.292"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..81
                     /locus_tag="ACHOQQ_RS21515"
                     /old_locus_tag="pgaptmp_004303"
     tRNA            6..81
                     /locus_tag="ACHOQQ_RS21515"
                     /old_locus_tag="pgaptmp_004303"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHOQQ_RS21520"
                     /old_locus_tag="pgaptmp_004304"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHOQQ_RS21520"
                     /old_locus_tag="pgaptmp_004304"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /gene="yhdZ"
                     /locus_tag="ACHOQQ_RS21525"
                     /old_locus_tag="pgaptmp_004305"
     CDS             complement(299..1057)
                     /gene="yhdZ"
                     /locus_tag="ACHOQQ_RS21525"
                     /old_locus_tag="pgaptmp_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGU4472478.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /gene="yhdY"
                     /locus_tag="ACHOQQ_RS21530"
                     /old_locus_tag="pgaptmp_004306"
     CDS             complement(1065..2168)
                     /gene="yhdY"
                     /locus_tag="ACHOQQ_RS21530"
                     /old_locus_tag="pgaptmp_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4472479.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /gene="yhdX"
                     /locus_tag="ACHOQQ_RS21535"
                     /old_locus_tag="pgaptmp_004307"
     CDS             complement(2178..3359)
                     /gene="yhdX"
                     /locus_tag="ACHOQQ_RS21535"
                     /old_locus_tag="pgaptmp_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4472480.1"
                     /translation="MSHRRSTVKGSLSFANPTVRVWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3427..4452)
                     /locus_tag="ACHOQQ_RS21540"
                     /old_locus_tag="pgaptmp_004308"
     CDS             complement(3427..4452)
                     /locus_tag="ACHOQQ_RS21540"
                     /old_locus_tag="pgaptmp_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4472481.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4883..5104)
                     /locus_tag="ACHOQQ_RS21545"
                     /old_locus_tag="pgaptmp_004309"
     CDS             complement(4883..5104)
                     /locus_tag="ACHOQQ_RS21545"
                     /old_locus_tag="pgaptmp_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472482.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1417 a         1333 c         1228 g         1171 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttatgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga cttaggatgc gcaaaaaact
      361 catccggtgc agcttgctcc actatttcgc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgggtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gatgtgggct
      541 catcaaacaa cataattttc ggcttcatac acagcgagcg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctaccac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaacctgaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacagaa cggtaatcag cggcacgccg
     1561 cgccagaatt cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaactga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagagc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcactg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccagttag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggatgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttccacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggt
     2401 ggaataatca cccgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat ccccgcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccgatgat caacactgcg
     2701 gcgatgggcc aggtgcggcg cagctgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttaagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccagaca
     3301 cgaaccgtag ggttggcaaa ggagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta atcttcagcg ggctttctga acctacgtta cgctcaaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccaacttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca tttagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagccggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggca acgttgagtt cggtatcagt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 ataagtgacg ccagtaaatg ccattcccat cccggcgtcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cttcccctga ctgtaaagcc gtgaagcgtt cttttgctgt
     4201 gagtggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aatatcaaca tcaatacctg aaaatttacc gtcagcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctga acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatg ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatac gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aagtgcgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaatcag cagagaatta
     4801 gccagcagaa tatagcaata ttttatctta tgtaaagata ctgtatcgtt aaacagagcg
     4861 cgaaactccg cgccctgttc agttatttcg acagagcgat gatccccatc gtcaggccag
     4921 caacagctgc tgctccgcca gcaatagccc cagccgtttc ccagttatcc tgagttgtgc
     4981 ctttcataca ggtattggtg tgaaccagac gcccctgatc gtcataaacc ggaacgcagg
     5041 gcgaatcgtg agcacagccc gccagcaatg cggtgagcaa tgccacagga attaatcttt
     5101 tcatggtgtt acctcagtaa taaacgctaa cgcttaataa gttaactct
//
LOCUS       DAZKFU020000291         5106 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZKFU020000291 DAZKFU020000000
VERSION     DAZKFU020000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.293"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            194..450
                     /locus_tag="ACHOQQ_RS21550"
                     /old_locus_tag="pgaptmp_004310"
                     /pseudo
     CDS             194..450
                     /locus_tag="ACHOQQ_RS21550"
                     /old_locus_tag="pgaptmp_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(472..745)
                     /locus_tag="ACHOQQ_RS21555"
                     /old_locus_tag="pgaptmp_004311"
                     /pseudo
     CDS             complement(472..745)
                     /locus_tag="ACHOQQ_RS21555"
                     /old_locus_tag="pgaptmp_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(742..2454)
                     /locus_tag="ACHOQQ_RS21560"
                     /old_locus_tag="pgaptmp_004312"
                     /pseudo
     CDS             complement(742..2454)
                     /locus_tag="ACHOQQ_RS21560"
                     /old_locus_tag="pgaptmp_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
     gene            complement(2447..3088)
                     /locus_tag="ACHOQQ_RS21565"
                     /old_locus_tag="pgaptmp_004313"
     CDS             complement(2447..3088)
                     /locus_tag="ACHOQQ_RS21565"
                     /old_locus_tag="pgaptmp_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGU4472483.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRVRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTER
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQRLTALLQPSQGNHPHD"
     gene            complement(3092..4009)
                     /locus_tag="ACHOQQ_RS21570"
                     /old_locus_tag="pgaptmp_004314"
     CDS             complement(3092..4009)
                     /locus_tag="ACHOQQ_RS21570"
                     /old_locus_tag="pgaptmp_004314"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4472484.1"
                     /translation="MTLLEKSLIVIFSLLLIATVINGLLVWLRPGKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPVDGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            complement(4006..4611)
                     /locus_tag="ACHOQQ_RS21575"
                     /old_locus_tag="pgaptmp_004315"
     CDS             complement(4006..4611)
                     /locus_tag="ACHOQQ_RS21575"
                     /old_locus_tag="pgaptmp_004315"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4472485.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIIAWNNVIWGVATLLLLWTIFNRCRSALREEGVK"
     gene            complement(4669..4854)
                     /locus_tag="ACHOQQ_RS21580"
                     /old_locus_tag="pgaptmp_004316"
     CDS             complement(4669..4854)
                     /locus_tag="ACHOQQ_RS21580"
                     /old_locus_tag="pgaptmp_004316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472486.1"
                     /translation="MLTQLHRVLYRCPGHYVYGSATQSTGTGTGSLASRNTSQSPLPA
                     QQAGFPRPELYSLSVCQ"
BASE COUNT         1343 a         1387 c         1261 g         1115 t
ORIGIN      
        1 taatcggcct ctaaaaaata tattgtatcc actttgttgt ggttctgaca gaagtaaatt
       61 aatttgaagt aaataaagca aaatatcaga agaatatatc ggggcgcaca gtctatctct
      121 gcctttgctt catgggatgt tctttgtata ttttacttgc tgtttaagta tgtatataag
      181 gagtgcattc attatgccaa tgaagcttgt aaagaagaat ccccggagca aagtgattcg
      241 ggagcttagc tgtgagcaag agatggcagt aaaggcggtc tgcggactga aaaaacagca
      301 actacggcac tacatgccct ggccaatgat cttctccggg aaatgcgtga gtatgatgca
      361 tggttacagt gtgactgtat tccgggagat tcgccgacca tgaattttgc tgctcttaaa
      421 aacaacacgg gaacttttta tctgtccagt ttggatatag ataattattg tactaataag
      481 ggaagccaac accagccgac agaccagaga cgccgctgct cattcagtga aacgcagata
      541 aacagtaaag caaatccaga ctccagtacg gaataatcgt cagcggtatt aacgggatcc
      601 ggtgctccca gtcaaacacc aggctgctga tgttttctct cccggcggta aactgattcg
      661 tcatgccata agtaagaaag aagaatgggg ccagcaccat tagccagccg attccctgaa
      721 taatcaccgc acgccgcgtt gtcatggttg acgtcgtacc gccatagaga ccgtgaagat
      781 gcccatttca tcaattagtt gcgtgcattt ctcaaaaccg gcgtcacgga ccagcgcgtc
      841 catctcgccc tgagtacgta cgcgcattac ccaggctttg ccatttttgt gactggtgag
      901 tgaccaggcg atggttttta actgagggtg ccagggctga caggtgtaga tcagaatgcc
      961 tcccggttca atcgctgcag ccagcccggc aagcgaatct cgtaccggcc cgttttccgg
     1021 gaacagttca tacagaccgg acacgatccc cagcgtcgga cgcggttcca gtgccgccag
     1081 ttcattacga ttaaaggcat cccctgctca aatcgcgcac ggcccgccat accgcgagag
     1141 gctatcatct cctgaccttt cgccacgttt aattcgctgt aatcgcgcag aaggatctcg
     1201 cttaccgccg tttcgccttg cagggcatca agcacatagc gtccatgccc ggcggcaata
     1261 tccacgatcc ggaccgagct accctgctct gttaaccgag caacagcctg ctgaattaac
     1321 atctgcagat gaattttacg ctggcggatc cccttccagc ccacgctatt caggtagttt
     1381 ttatcaatga ggcgtcccaa cgaggtgctg ccctgaggct ggttgcgata gatgtagtct
     1441 agggtactgc cggaatcgaa accagtctca aatcccagac gcacccccgt ggactgcgtg
     1501 cccagcactt tcatcccgta gcgcaaacag cggtaagaga tatcgtccag cgaatacagc
     1561 gcagggccac cgctcagtat gcgccaggta tcggcgctgg ggctccagcg atcttcctgg
     1621 ctgtaatcaa aacgctgcac gggacgggca tacaaggtat caataaaact gcgcatcctg
     1681 tcaaatgcca gatgccgctc tttttcgcct aaggtgtcat gataaaatcc cggcagtaca
     1741 tgcagttctt tctgcggatt tcgcaggttc tgataaaaat caagctgcgg ctggcggtgc
     1801 acaacgtaat cttccccgga gaccagcaac tgcacaggta gcgtaatggc gctggcatcg
     1861 ctgacgactc gctcagccgt tttgtagaga tcaagcagaa tattcacagc aattggtcgg
     1921 gtaatcagcg gatccttatt gaagcttgcc acccgctcgg gatcgtgcgt cagatacttg
     1981 cctttcacat acgagttgac gaagaaaaga ccgcgcaggc gatacatcaa ttccagcccc
     2041 tggcgagcaa acggcacata caatttgacc ttaaacgccg gtgatgccaa caccaggcca
     2101 cgaatgtccg gggcataatc atgcgcccag gttgccgcca gaaccgcccc gacgctttgc
     2161 gccacgacga ccacatccgt catcccagct tggctatctg cggcggcgaa acgcacaaac
     2221 tcgtcaacat cctgcaccga acgcacgagc gacggactgt aacaacgctg tatccggcat
     2281 cgccagctca tcaacaatgt gttgcaaacg cccggagtgc tcatgcccac gatgaaacag
     2341 gaccaccact tttggcgttg aactggcttc ttgtgctggc cagtggcgat aaaacagctc
     2401 tgtattatca ctggtcagaa actgccgttc atcgggtatg cgggaattag tcatggggat
     2461 gatttccttg cgagggttgc aaaagcgccg ttagtctttg ctccaggtcg tccataaacg
     2521 ttttcttctc cggatgcgta cttagcgcat cgccaatatt tacatcaata aacagcggca
     2581 aagggatacg attacctttc gccatcacac gctcagtacc gcgcaaccca acggggataa
     2641 tgggcacctc cggcatgcgc tgactcaaat gccacactcc ggatttcaat ggcgcaagta
     2701 cgcctggctc accgcgcgtc ccttccggaa acaggatcag tgttttacct tcacgcagcg
     2761 cctgttcaca ttgcgccagc ggatcggtat aaccgccctt tcttgaaata ggaatgatgt
     2821 taaggatctt cagcgcaaac caagccatgc gtttatcccg cagaaaataa tccgcggcag
     2881 caaccggatg aacagaagcc tggtaacgca gcggaaaaag cgaaagcaag gcgaaaacat
     2941 ccatatggct gttgtgattt gccacgatta tcgaaggccc gtgcttcggc agtcgctcgc
     3001 ggtggcggac acgcagacca agccacagcc agatgaccgg ccagacgata cacagcgtaa
     3061 acagggtacg gagtagtcgt gtcatcatag gttagtagca gaaatagcga acaaaatgga
     3121 agaagaccgg tgcggtaaaa atcagcgaat ccagccgatc aaggatgcct ccatggccgg
     3181 gtagcagcgt accactgtct ttgacgccga tatcacgctt gattgccgac atcaccacgt
     3241 cgccgcaaaa gccagtaacg ccgataataa ccccggctaa cagtgccatc ggccagctca
     3301 ggggagtcaa cagtggcccc aggactgttg ccgccagcgc cgtcgttgcc accccaccca
     3361 gcaatccctc cagggttttg tttgggctga ccttcggtat gacttttata cgaccaagcg
     3421 acttgcccca caggtattgc gcgatgtcgt taaattcggt caggccgacc aaaaacagca
     3481 ccaacagcgc gctggtttgc attccatcca ccggtaacac cagtaaaaac gcgacatggc
     3541 tgaaggcaaa tacggtggtc atcagactcc agtgcaactg ggaaacggta cgtaaaaagc
     3601 cctgtgtatc cccagcaatc accattcgcg ccggtaaaaa aaggaacacg tagaccggga
     3661 taaacaccac aaacatgccg taccagccga tacctatcag ccagtagtta aggggaattg
     3721 caataaacat ccataacagc ggcatgcggt cggattggcg ggaaggcacc agcgtcaaaa
     3781 actcttttag cgccataaag ctgaccagcg cgaagaacgt cagtgccagc cagctaggac
     3841 ttaatagtgc gaaggaaaac aggacgataa tcacccacca ggtgcgaata cgtaatgtca
     3901 gctcgcgcca gtctttacct ggacgcagcc acaccagcaa cccgttgatt acggtggcaa
     3961 tcaacagcag ggaaaaaata acgatcagtg atttttcaag tagcgtcatt tcacaccctc
     4021 ttcacgcaat gcgctgcgac agcgattaaa aatcgtccat aacaacaaca gtgttgcgac
     4081 accccatatg acattattcc aggcgataat ctgcggccag atcgacagtg acagccccca
     4141 tgcaccaaac accagcgctc tgtcgctttt gcccagcgga ccggcatagc tgcgcacgcc
     4201 gttaatcgtt tgcgccagca cgccgcaaaa ctcggtcatc agcgcacaaa acaccacgat
     4261 cagcaccagc ggtatactgc tctgcgggag tagcataaag ggcagataaa gcgagatgtc
     4321 ggaaattaca tcacccgtct cattgaggat ggcccccaga cgcgatcttt gattacattc
     4381 acgcgccagc ataccatcga gcgcattgag cgccattcgt acaaacaaca ccacgggcag
     4441 aaggacgaat aatccgggct gaggatgaat aatgagcacc acgccggtga taagggaaag
     4501 caccatcgcg cacagtgtaa tgtgattagc ggtgacgccg cgcctgttca gccagaacat
     4561 cagcggtctt aacagagcct gaaacgaggg ctttatctgg taaagggtca tcatcactcc
     4621 ttaataagca tatttgacgg ggttgagtct ggccttttat tggggctgtc actggcacac
     4681 tgaaagtgaa tacagttccg ggcgcggaaa tcctgcctgc tgcgccggta aaggcgactg
     4741 actggtatta cgggaggcca gagagccagt acctgtgccg gtagactgtg tggctgaacc
     4801 ataaacatag tgtcctggac aacggtacag cactctgtgc agctgtgtca gcatgcgctt
     4861 ttctttaatt atgcccagtc atgtgcggag gcaaggattt caaaatatcc gttacatttg
     4921 ttgcatgatg ccttatcgct ggtccgggct actgctttac ttcttttaaa tcagctctga
     4981 tgaacatttt ttaccacttt atcgcattaa ggttatgtca ttcatcgtct ggcatcacgt
     5041 ttttggttat ggtgtcaggg tgtaccctac cgcgataacg caaccctaat aaagtcattc
     5101 gatggc
//
LOCUS       DAZKFU020000292         5103 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZKFU020000292 DAZKFU020000000
VERSION     DAZKFU020000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.294"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..133)
                     /gene="ypaB"
                     /locus_tag="ACHOQQ_RS21585"
                     /old_locus_tag="pgaptmp_004317"
     CDS             complement(<1..133)
                     /gene="ypaB"
                     /locus_tag="ACHOQQ_RS21585"
                     /old_locus_tag="pgaptmp_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGU4472487.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     "
     gene            261..2546
                     /gene="nrdA"
                     /locus_tag="ACHOQQ_RS21590"
                     /old_locus_tag="pgaptmp_004318"
     CDS             261..2546
                     /gene="nrdA"
                     /locus_tag="ACHOQQ_RS21590"
                     /old_locus_tag="pgaptmp_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4472488.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2780..3910
                     /gene="nrdB"
                     /locus_tag="ACHOQQ_RS21595"
                     /old_locus_tag="pgaptmp_004319"
     CDS             2780..3910
                     /gene="nrdB"
                     /locus_tag="ACHOQQ_RS21595"
                     /old_locus_tag="pgaptmp_004319"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4472489.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            3910..4164
                     /gene="yfaE"
                     /locus_tag="ACHOQQ_RS21600"
                     /old_locus_tag="pgaptmp_004320"
     CDS             3910..4164
                     /gene="yfaE"
                     /locus_tag="ACHOQQ_RS21600"
                     /old_locus_tag="pgaptmp_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGU4472490.1"
                     /translation="MARVILRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4218..4868)
                     /gene="inaA"
                     /locus_tag="ACHOQQ_RS21605"
                     /old_locus_tag="pgaptmp_004321"
     CDS             complement(4218..4868)
                     /gene="inaA"
                     /locus_tag="ACHOQQ_RS21605"
                     /old_locus_tag="pgaptmp_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGU4472491.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIGGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(4949..>5103)
                     /locus_tag="ACHOQQ_RS21610"
                     /old_locus_tag="pgaptmp_004322"
     CDS             complement(4949..>5103)
                     /locus_tag="ACHOQQ_RS21610"
                     /old_locus_tag="pgaptmp_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4472492.1"
                     /translation="ACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALV
                     VAKEIH"
BASE COUNT         1247 a         1387 c         1266 g         1203 t
ORIGIN      
        1 ccgaacagtt atttttaaca aatttttctc ttcccattga ctttcccgga caccttgtct
       61 gacctaaggt gcgcgaaagc cactttttcc ttcctgagtt atccacaaag ttatgcactt
      121 gcaagagggc cattttcaca ctatcttgca gtgaatccca aacatacccc ctatatatag
      181 tgttctaagc agcttcccgt actacaggta gtctgcatga aactattgcg gaaagaattc
      241 caaaaacagg tacgacatac atgaatcaga atctgctggt gacaaagcgc gacggtagca
      301 cagagcgcat caatctcgac aaaatccatc gcgttctgga ttgggcggca gaaggactgc
      361 ataacgtttc gatttcccag gtcgagctgc gctcccacat tcagttttat gacggtatca
      421 agacctctga catccacgaa accattatca aggctgccgc agacctgatc tcccgtgatg
      481 cgccggatta tcagtatctc gccgcgcgcc tggcgatctt ccacctgcgt aaaaaagcct
      541 acggccagtt tgagccacct gcgctgtatg accatgtggt gaaaatggtc gagatgggca
      601 aatacgataa tcatctgctg gaagactaca cggaagaaga gttcaagcag atggacacgt
      661 ttatcgatca cgaccgtgat atgaccttct cttatgctgc cgttaagcag ctggaaggca
      721 aatatctggt acagaaccgt gtgaccggcg aaatctacga aagcgcccag ttcctttata
      781 ttctggttgc tgcgtgcctg ttctcgaact acccgcgtga aacgcgcctg caatacgtga
      841 agcgttttta cgacgcggtt tccacattta aaatttcgct gccgacgcca atcatgtccg
      901 gcgtgcgtac cccgactcgt cagttcagct cctgcgtact gatcgagtgc ggcgacagcc
      961 tggattccat caacgccacc tccagcgcga ttgtgaaata tgtttcccag cgtgccggga
     1021 tcggcatcaa cgccgggcgt attcgtgcgc tgggtagccc gattcgcggt ggcgaagcgt
     1081 tccataccgg ctgcattccg ttctacaaac atttccagac tgcggtgaaa tcctgctctc
     1141 agggcggcgt gcgcggaggt gcggcaacgc tgttctaccc gatgtggcat ctggaagtgg
     1201 aaagcctgct ggtgttgaaa aacaaccgtg gtgtggaagg caaccgcgtg cgccatatgg
     1261 actacggggt acagatcaac aaactgatgt atacccgcct gctgaaaggt gaagatatca
     1321 ccctgttcag cccgtccgac gtaccggggc tgtacgacgc attcttcgcc gatcaggaag
     1381 agtttgaacg cctgtatacc aaatatgaga aagacgacag catccgcaag cagcgtgtga
     1441 aagccgttga gctgttctcg ctgatgatgc aggaacgtgc atctaccggt cgtatctata
     1501 ttcagaacgt tgaccactgc aatacccata gcccgtttga tccggccatc gcgccagtgc
     1561 gtcagtctaa cctgtgcctg gagatagccc tgccgaccaa accgctgaac gacgtcaacg
     1621 acgagaacgg tgaaatcgcg ctgtgtacgc tgtctgcttt caacctgggc gcaattaata
     1681 gcctggatga actggaagag ctggcaattc tggcggttcg tgcacttgac gcgctgctgg
     1741 attatcagga ttacccgatc ccggccgcca aacgtggagc gatgggtcgt cgtacgctgg
     1801 gtattggtgt gatcaacttc gcttactacc tggcgaagca tggtaaacgc tactccgacg
     1861 gcagcgccaa caacctgacg cataaaacct tcgaagcgat tcagtattac ctgctgaaag
     1921 cgtctaatga gctggcgaaa gagcaaggcg cgtgcccgtg gtttaacgaa accacttacg
     1981 cgaaagggat cctgccgatc gatacctata agaaagatct ggatgccatc gctaatgaac
     2041 cactgcatta cgactgggaa gcactgcgtg aatcgatcaa aacgcacggt ctgcgtaact
     2101 ccacgctttc tgccctgatg ccgtccgaga cttcttcaca gatctctaac gccactaacg
     2161 gtattgaacc gccgcgcggt tacgtcagca tcaaagcgtc gaaagacggt attctgcgcc
     2221 aggtagtgcc ggactacgag catctgcacg acgcctatga gctgctgtgg gaaatgccgg
     2281 gtaacgatgg ctacctgcaa ctggtgggta tcatgcagaa atttatcgat cagtcgatct
     2341 ctgctaacac caactacgat ccgtcacgct tcccgtcagg aaaagtgccg atgcagcaat
     2401 tgctgaaaga cctgctcacc gcctacaaat tcggggtcaa aacgctgtat tatcagaaca
     2461 cccgtgacgg cgctgaagac gcgcaggacg atctggtgcc gtcaatccag gacgatggct
     2521 gcgaaagcgg cgcatgtaag atctgatatt gagatgccgg atgcggcgta aacgccttat
     2581 ccggcctacg gctcggtttg taggcctgat aagacgcgcc agcgtcgcat caggctccgg
     2641 gtgccggatg cagcgtgaac gccttatccg gcctacggct cggatttgta ggcctgataa
     2701 gacgcgccag cgtcgcatca ggcacaggat gcggcgtaaa atgccttatc cggcattaaa
     2761 ctcccaacag gacacactca tggcatatac caccttttca cagacgaaaa atgatcagct
     2821 caaagaaccg atgttctttg gtcagccggt caacgtggct cgctacgatc agcaaaaata
     2881 tgacatcttc gaaaagctga tcgaaaagca gctctctttc ttctggcgtc cggaagaagt
     2941 tgacgtctcc cgcgaccgta tcgattacca ggcgctgcct gaacacgaaa aacacatctt
     3001 tatcagcaac ctgaaatatc agacgctgct ggactccatt cagggtcgta gcccgaacgt
     3061 ggcgctattg ccgcttattt ctattccgga actggaaacc tgggtcgaaa cctgggcgtt
     3121 ctcagaaacg attcattccc gttcctatac tcatatcatt cgtaatatcg ttaacgatcc
     3181 gtctgttgtg tttgacgata tcgtcaccaa cgagcagatc cagaaacgtg cggaagggat
     3241 ctccagctat tacgatgagc tgatcgaaat gaccagctac tggcacctgc tgggcgaagg
     3301 cacccacacc gttaacggta aaaccgtaac cgttagcctg cgcgagttga agaaaaaact
     3361 gtatctctgc ctgatgagcg ttaacgcgct ggaagcgatt cgtttctacg tcagctttgc
     3421 ttgctctttc gcattcgctg aacgtgagct gatggaaggt aacgccaaaa ttattcgcct
     3481 gattgcccgc gacgaagccc tgcacctgac cggcacccag catatgctga atctgctgcg
     3541 cagcggcgcg gatgatccgg agatggcgga aattgccgaa gaatgtaagc aggagtgcta
     3601 tgacctgttt gtccaggcgg cacaacagga gaaagactgg gcggattatc tgttccgtga
     3661 cggttcgatg attggtctga acaaagacat tctctgccag tacgtggagt acatcaccaa
     3721 tatccgtatg caggcggttg gtttggatct gccgttccag acgcgctcca acccgatccc
     3781 gtggatcaac acctggctgg tgtctgataa cgtgcaggtt gctccacagg aagtggaggt
     3841 gagttcttat ctggtcgggc agattgactc ggaagtggac accgacgatt tgagtaactt
     3901 ccagctctga tggcccgcgt tattctgcgc atcactggca cacaactgct gtgccaggat
     3961 gaacaccctt cccttctggc ggcgctggaa tcccacaatg tggcggttga gtaccagtgc
     4021 cgcgaaggtt actgcggctc ctgtcgcacg cgccttgttg caggccaggt ggactggatt
     4081 gccgaaccgt tagcctttat tcagccgggg gaaattttgc cctgttgttg ccgggcaaaa
     4141 ggcgatattg aaatcgagat gtgaattggt tgtagtgcca gatacaacgc ttatgcgtat
     4201 tatctggcct acaacgatta catggcgtag taatacgctt ttacctgctc ccagtccgct
     4261 ttggggatcg gctccagata tttttccagc tggcggaagt catgattaat cgctttatcg
     4321 cgacgcaagc ggcgacgact tttctccaga tcaagaaaac cagcttctgc cttaccttct
     4381 gttttcacat agatatggcg aacataacaa cagccatgct gacgattaac gctatgcatt
     4441 ttcttaaacg ccagcgccac tgctttcaac atggcttgcc gtacttcgtc agaatagggc
     4501 gttactgcat gctgggcata ccagtcagca atgctgatga accccgccat atcttcagtc
     4561 accagcaacg cgcgccattc acccccaatt ttcaccgctt caccaaaaac gatcttcggc
     4621 acgatgacac cggcccgttc cagttcttta attaccgcaa cctcacggac aatcgttggt
     4681 cggccgaaag gataacgtac ggaatgaaac agatgatgcg tcatgcgctt tacatatagc
     4741 tttttgccgt tgcgctcgac gcattgcacc ccgctcatac cattacggcg atagttaggc
     4801 tcttcaaccc agtcgccctc tgttgcccac cagtgattaa attcgtcgtg ctttgctgaa
     4861 actgccatac cctatcgcct gtcatttttt attaacgaca atgactatag gtggttacct
     4921 gcgaaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccact actagcgcga
     4981 caccgctttt acccttcatt tgcagcgcat agactccacc agccactacc actaatagtg
     5041 cgcccagcag ataaatcatt cggtagccag tatgtgcaac catcatcccc atcacacagg
     5101 ctc
//
LOCUS       DAZKFU020000293         5074 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZKFU020000293 DAZKFU020000000
VERSION     DAZKFU020000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.295"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            171..548
                     /locus_tag="ACHOQQ_RS21615"
                     /old_locus_tag="pgaptmp_004323"
     CDS             171..548
                     /locus_tag="ACHOQQ_RS21615"
                     /old_locus_tag="pgaptmp_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472493.1"
                     /translation="MYSPLIAHYSALQTQSALLADISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            630..1259
                     /locus_tag="ACHOQQ_RS21620"
                     /old_locus_tag="pgaptmp_004324"
     CDS             630..1259
                     /locus_tag="ACHOQQ_RS21620"
                     /old_locus_tag="pgaptmp_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472494.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TTPVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            1375..1758
                     /locus_tag="ACHOQQ_RS21625"
                     /old_locus_tag="pgaptmp_004325"
     CDS             1375..1758
                     /locus_tag="ACHOQQ_RS21625"
                     /old_locus_tag="pgaptmp_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472495.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            1851..2828
                     /locus_tag="ACHOQQ_RS21630"
                     /old_locus_tag="pgaptmp_004326"
     CDS             1851..2828
                     /locus_tag="ACHOQQ_RS21630"
                     /old_locus_tag="pgaptmp_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HGU4472496.1"
                     /translation="MKTKKHTSRTATKTPEHDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKPYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMETVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSSDALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYWTTLLHELVHSTGHEKRLRRVGISTFTGRKSPEYAFEELIAEMGSAFLCAHLGI
                     AGDVQHESYIDGWLKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
     gene            2938..3372
                     /locus_tag="ACHOQQ_RS21635"
                     /old_locus_tag="pgaptmp_004327"
     CDS             2938..3372
                     /locus_tag="ACHOQQ_RS21635"
                     /old_locus_tag="pgaptmp_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HGU4472497.1"
                     /translation="MNDNALVDVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKEGLQSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            3376..3690
                     /locus_tag="ACHOQQ_RS21640"
                     /old_locus_tag="pgaptmp_004328"
     CDS             3376..3690
                     /locus_tag="ACHOQQ_RS21640"
                     /old_locus_tag="pgaptmp_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472498.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKAELWYYNLFINPSADELMDVNRRYGPERTAWLQGFIRNN
                     GA"
     gene            3774..4721
                     /locus_tag="ACHOQQ_RS21645"
                     /old_locus_tag="pgaptmp_004329"
     CDS             3774..4721
                     /locus_tag="ACHOQQ_RS21645"
                     /old_locus_tag="pgaptmp_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU4472499.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRQGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPRLYLVEECAEEQTKAAPLPVPLVSAQ"
     gene            4833..>5074
                     /locus_tag="ACHOQQ_RS21650"
                     /old_locus_tag="pgaptmp_004330"
     CDS             4833..>5074
                     /locus_tag="ACHOQQ_RS21650"
                     /old_locus_tag="pgaptmp_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HGU4472500.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLE"
BASE COUNT         1136 a         1174 c         1495 g         1269 t
ORIGIN      
        1 gaaggatgtc aaatcgtgag cgatgcaact gccattttgc aatgatggct gttttgctcc
       61 cgtaacggtt tttcttttac ttactgcacc gtgtttttgc gccggtgttt tccctgacgg
      121 gagttttgtt ccgtcaggga caggacttcc tttaagagac ggaggtcatc atgtattcac
      181 ctttaattgc tcattattca gcgcttcaga cgcagtctgc tctgctggct gatatcagtc
      241 agatggaagg cgagctgatg cggttacgtg cctggcaacg gctgggtatt accccggagc
      301 cggatgatga gacggagcct tcactggtat acgtgtatct gtgggatacc ggcgaggcgc
      361 tgggctggct gttgtatgac ctggagcagg aggagatttc cgcaccggga attcaggccc
      421 gtcaggcact cgacagtctg atggctctgg ggcgtgaaat taatcatgaa atctgggctg
      481 acagtatcag taccggaaag atggtggccg gagcggatga atattttgcc cggcacgaca
      541 tgatgtaatt catgacctga ccctgacggg ggagttctcc gtccgggaga cattccccgt
      601 tgtttcgttt taaccatgag gaatgttcca tgtctgagtt atcttcacgt ccggcccgtg
      661 agccggtggt atataccctt gagcaggttt ccacaatacc ggaaaagcag tggcatgctt
      721 ttgttctggc tgtcactgaa accttctggc agttgccgga ggcgttacgg ccgcagaatg
      781 cgtatttcgg ctccctgacc cgggcgtcag agttgtttcc ggtaacggac acgctggcgt
      841 tttactgccg gtctgccgac gggctgtggt cggtgaatgt cactattgag cgggagcaca
      901 gccggaatat cctggctctt aatgagctga atttcggacg tcagccggga gatttctttg
      961 cccgtacagt ctttgtgctg ttgcacaatc tgtgtccgga ctgttttcgt attcattcca
     1021 ctgtcggtgg tgccagctgg tcgctgccgc tgaaatggat aaaacggtac ctgggacatg
     1081 agaatttttc tgctccggag tctgtattaa ccaccccggt aaggggagat gcgtttgaca
     1141 gcttactgct gcagtttttg tccggtcagg ggcgacagtt atccccggat gactgggcag
     1201 cactggaaga ggccgaatat cagctttact ggctgcgggc tcttgccggt gggcggtaac
     1261 agaacgccgt agtttcttta tcaggaataa cgtcatcacg tattccgtca ttatatcacc
     1321 cggcagggac atatccctgt ggggagagtc ccggctttat tcaggagaat tgttatggga
     1381 ctcgatattt actttctggc tcgtgataac catcacagtg gtgccgttgt gggaacacag
     1441 gaacatcagg aggtgggggc tttccgtaag gtgaaaccgc ttctgacatg gtttcgtgaa
     1501 cataccggtg cttcagatga gcagcatgct ttccctgtaa cccgcagttc actggctgca
     1561 ttattgcgcg acctcagtat gctgacaccg gggaactgcc acaggctgtt tccttatgtc
     1621 acaggctcgt gtttacgggg ggaagattat gccggtgatt actggtatga cgtggatgac
     1681 gtaaaagcgt gggtcagcaa tacgctgaag acgtttgatt ttgagcgtcg ttcactggtg
     1741 atgctggcgt tgtggtaatt ctggccagtc tgattttcgt tattaattta actgtacggt
     1801 gcactctcac cgtacaggga gcgtgcatct ttttcagggg gcatgctctt atgaaaacga
     1861 aaaaacatac ttcacgtacc gcgactaaaa ctccggaaca tgacctgtat cagcttgtga
     1921 ccgacagggt tgttacggcc attgaaaatg gtgtgccacc ctggaaacgt ccctggcgct
     1981 ctgcgcgtaa tggcgtgggg cttccggaga tgatgccggc aaatgccctg accgggaagc
     2041 cttatacagg ggtgaacgtc ctgttgctgt ggctggccgc ggatgaagcc gggtatgtca
     2101 gcaatgactg gctgacgtac cgtcaggcgc aacaactggg tggccacgtc cgtaaagggg
     2161 aggtggccac gctggcggtg atttacaaag actggaaaaa acaggctgtg gatgaacacg
     2221 gtaaccggct gacggacagt gagggggagc ctctcatgga aaccgtaccg gtactgaaat
     2281 cgaatcctct gtttaatgtg gggcagtgtg acggtttacc tgaacatctg atggtgaagg
     2341 cggaaggcag ctcggatgca ctgccggcag ataccttcag tgagcgggtg ctgcaggtac
     2401 tgaatggcag cggcgtcagc gtctcgtcac ttccacagga cagggcattc taccggcctg
     2461 atcttgaccg tatcgtgatg ccactgtcgt cgcagtttga ccaggtggct gattactgga
     2521 ccaccctgct gcatgagctg gtacacagta ccggtcatga aaagcgcctg cggcgggtgg
     2581 ggataagtac ctttaccggt cgcaaaagcc cggaatatgc gtttgaagag ctgattgccg
     2641 agatgggcag tgcttttctg tgtgcacatc tcgggattgc cggtgacgta cagcacgaaa
     2701 gttatattga cggatggctt aaggttctga agtctgacaa aaaagcgctg tttcgcgcct
     2761 gccggcaggc acgtgaagca tcagagtatt tactgacgcg gcgaacttcg caggagtgcg
     2821 cagcataatt tttactaccg gtggcggtta taacctgacc gccatttatc ccgtgagggc
     2881 attgtccctc acggggcagt gctctctgtt atttcaggat tacagaggaa ctgagtgatg
     2941 aacgataacg cactggttga tgttgacgat gctgttgtgg cccgtatcag ggatgtgctg
     3001 gcgtatacat ttccgggaca gaaattttct gtgcggaaat gcgatccatc tttcccgaaa
     3061 gttttcaaca atgtgtgggt gagctggctg gatggtccaa cggaggccga agtacgctct
     3121 gtcacagagg ggtttgagaa taaagagggg ctgcagtctg tgcttgaatc ccgttatttc
     3181 agcgatgaac tggtacagga gtgtattgat cgccttcgta aagagtacgg acaggggaac
     3241 gtcccgttga gtgtgaccgt ggagcgttac cggaaaaacg agctggagaa gattgaaaca
     3301 gcgcgttttc cgggcaacat atccgtagcc ataaacgaga tcgggataaa taccagcaaa
     3361 tacaggaggt aaggtatggc tggtaaatgc cttgtctggt tctgtaacgg gatctctgaa
     3421 ccgtgtctga tagcaggaaa tatcagtgaa ttactggcag atgttacgat cacgtcaaat
     3481 taccgtggag atgtcacatc tgaaatcagg gatgttgtac aggagtggct caggaccggc
     3541 agggggaacc tggcggattt aaaggcagag ctctggtact acaacctttt catcaatccg
     3601 tctgcagacg aactgatgga tgtcaacagg cggtatggcc ctgagcgaac tgcctggctg
     3661 cagggtttta tccgcaacaa cggggcgtga taccccgttt tcccgtgagg gcattgtacc
     3721 tcacgggaca atgccctctt tttacatctg tataaaaaag aggagcgtaa acaatgtctg
     3781 aatggtgcaa aaaccgtttt gaaattaccg gtaaatcagt ctgtcttgat gtacttacac
     3841 agtggattga agggcgtgag accccgcgct accgtcatgc catacagcag agtatacagc
     3901 tgtttctggc cggttgtgca gggctggtaa aacccacacg gacgacgcag tatccgccat
     3961 atccggcgct ggttcgtcaa ggaacggggt tgtcatctcc ggctaaccag gcgtttgagc
     4021 agtggctggg gttgctggta aaaaatgccg ttctggatgg tgaaacgata aagaccattg
     4081 agcgtcttta tcaccagtca tgtatcggcg ctgttcgctg gggtaatctg ccggataatg
     4141 ccagggagat catcaccacg ctgatgagcc gtcagtattc tgactggttt ggtctggccg
     4201 ggctgagtga acacattgat gcagaagcct gctggagctg gttgtcggat tacccggaac
     4261 aggcgcagcc ctgtgacatg ctgatggtca taccctcccg tctggctaca gagctgaatg
     4321 gttcaggtgg actgctgcag ggtatttcca caacgaccag cctttacagc cggatatacg
     4381 gtgtggagtg gccgtcagga aataatgttc gccatgcgcg tgatgagatg agcagtctgg
     4441 tgctgctgat cgacacgccc tggtatccgc cttcaggcga actggttggt gagatctcga
     4501 aggtgtttga ctgcgaggtc cgacactggt acagcgagcc ggtacacggt atacagggtt
     4561 acaactgtta cgatggcgga gagcatacgg acagtgatct gcaggccgaa tggccgggca
     4621 gggaaacact tccacagccc cgactgtacc tggtggagga gtgcgctgaa gaacagacga
     4681 aagccgcacc tttaccggtt cctctggtct cagcccagta attaacctcc gggtatcatg
     4741 tccgtgatgc ccgttttatc aacatttcaa cccgaagggg aacacctgtt cccctgtccg
     4801 ggccatgtgt tccctgtttt tacggagaaa acatggccac catgactgac catcagaagg
     4861 agttcatttc gttgtttaac cggacagcct gttatcacaa acgtcataag gtgtttgaag
     4921 actttgtcag ctgtagtgtg attgccctgg aaaaccggct tcagttcagt gaggcccggg
     4981 aacagaagta tctgcggatc gtcagtggtt atgaaaagca ggatgttatc cacattgcgc
     5041 atttgcttgc tcatgtcatt gagggactgg agca
//
LOCUS       DAZKFU020000294         5071 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZKFU020000294 DAZKFU020000000
VERSION     DAZKFU020000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.296"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            123..1481
                     /gene="mltD"
                     /locus_tag="ACHOQQ_RS21655"
                     /old_locus_tag="pgaptmp_004331"
     CDS             123..1481
                     /gene="mltD"
                     /locus_tag="ACHOQQ_RS21655"
                     /old_locus_tag="pgaptmp_004331"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGU4472501.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1529..2152)
                     /locus_tag="ACHOQQ_RS21660"
                     /old_locus_tag="pgaptmp_004332"
     CDS             complement(1529..2152)
                     /locus_tag="ACHOQQ_RS21660"
                     /old_locus_tag="pgaptmp_004332"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4472502.1"
                     /translation="MSGLPQGRPTFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKN
                     ITTRHGYAESLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQTVEALRDTSHVRNYASCEWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQNDGSFTSDIIMVEAHKAA"
     gene            complement(2156..2956)
                     /locus_tag="ACHOQQ_RS21665"
                     /old_locus_tag="pgaptmp_004333"
     CDS             complement(2156..2956)
                     /locus_tag="ACHOQQ_RS21665"
                     /old_locus_tag="pgaptmp_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4472503.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3197..4111
                     /gene="yafC"
                     /locus_tag="ACHOQQ_RS21670"
                     /old_locus_tag="pgaptmp_004334"
     CDS             3197..4111
                     /gene="yafC"
                     /locus_tag="ACHOQQ_RS21670"
                     /old_locus_tag="pgaptmp_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGU4472504.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGALTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4108..4911)
                     /gene="dkgB"
                     /locus_tag="ACHOQQ_RS21675"
                     /old_locus_tag="pgaptmp_004335"
     CDS             complement(4108..4911)
                     /gene="dkgB"
                     /locus_tag="ACHOQQ_RS21675"
                     /old_locus_tag="pgaptmp_004335"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGU4472505.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVSRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1349 a         1214 c         1256 g         1252 t
ORIGIN      
        1 caacctgaag aacgttttgc atggttaagg tcaaagaaag ataggttctg ataaaacttt
       61 cttgtcatcg gctccgttcg ccgttatgat cggtcgtctt ttaagcaact attgacacac
      121 acatgaaggc aaaagcgata ttactcgcct ctgtcctgct cgtgggttgc cagagtaccg
      181 gcaacgttca acagcacgca cagagccttt ctgcagctgg tcaaggggaa gcagcaaagt
      241 ttacaagtca ggcacgatgg atggacgatg ggacgtctat cgcgccagat ggtgacttgt
      301 gggctttcat tggcgacgag ctaaagatgg gaattccgga aaatgaccgg attcgcgaac
      361 agaaacagaa atatttacgc aataagagct atctccacga tgtaacttta cgggcagagc
      421 cgtatatgta ctggatagcc gggcaagtta aaaaacgtaa catgcctatg gaactggtac
      481 tactacccat agtggagagc gcttttgatc ctcacgcaac gtctggcgcc aatgccgcag
      541 gcatctggca gatcattccg agcacggggc gcaattatgg tttgaaacag acccgcaatt
      601 atgatgcgcg tcgcgatgtt gttgcttcaa caactgccgc gctgaatatg atgcagcgtc
      661 tgaacaaaat gtttgacggc gactggcttc tgaccgtagc ggcttataac agcggcgaag
      721 gtcgggtcat gaaggcaatt aaaacgaaca aagcgcgtgg gaaatccacg gacttctggt
      781 cgttaccgtt gccgcaggaa acgaagcagt acgtgcctaa aatgctggca ttgagtgata
      841 ttctcaaaaa cagcaagcgt tatggcgtac gtctgccaac gaccgatgaa agccgtgctc
      901 tggcgcgtgt gcacctgagc agcccggttg aaatggcgaa ggttgcagat atggcgggga
      961 tttccgtcag caagctgaag acattcaacg ctggcgtgaa aggctccacg ctgggcgcaa
     1021 gtggtccgca atacgtgatg gtgccaaaga agcatgcaga tcaactgcgt gaatctctgg
     1081 cttcaggcga aattgctgct gtacagtcga cgctggttgc cgacaatacg ccgcttaaca
     1141 gccgtgttta caccgtacgc tctggcgaca cgctttcaag tatcgcttca cgtctcggcg
     1201 taagcaccaa agatttgcag cagtggaaca aactgcgcgg atctaagctg aagccaggcc
     1261 aaagtttgac gattggtgca ggcagtagcg cacagcgact ggcaaacaac agcgatagca
     1321 ttacgtatcg tgtgcgcaaa ggcgattcgc tttcaagcat tgctaaacgc cacggcgtga
     1381 acatcaaaga tgtgatgcgc tggaacagcg acactgcgaa tctgcaacca ggcgataagc
     1441 tgacgttgtt tgtgaaaaac aacaacatgc cagattcctg acaaaccaga taataaaaag
     1501 gcaccgattc ccccggtgcc ttttttattt atgccgcttt atgtgcttct accatgatga
     1561 tatcactggt gaaagagcca tcattctgca aggaaaaata cgttctcacc tctgtcgatg
     1621 cgctctgttg gtaaatgcga atagcgtcta ctaacgcttc tggcgtacgc attctcacga
     1681 cccatgaaga aaattccagc ggtaacttat ctgtaattaa attatcaact atcagattgg
     1741 cttcattgat taacgtcaac cactcacagc tggcgtagtt tcgtacgtga gaggtatcgc
     1801 gtaatgcttc taccgtctgt aaccagatgt cgcgcactgg gtggcccgga gacattacgt
     1861 ccatcacaat cagcctaccg ccaggtttca atatcctatt cacttctcgc agtgctgcgc
     1921 cgacatcatg ccagtgatgg gcagaataac ggctgataac aatatcaaat gcgttatcgg
     1981 caaatggcag actttcggca taaccatgac gagtagtgat atttttcagt tgccgggctt
     2041 cggcagcttg tgccacgaca tccagcattt gggcagataa gtcatacgcc accaccgcgc
     2101 tcacgttttg cgcagcgaca aacgttggtc tgccctgtgg cagacctgac ataccttatt
     2161 tatcaggctt gccgggactg aattcaacga gtagcggatt gtgatcggaa gcgcgcgtaa
     2221 ccagtacaga agcttcactg acgttcagac cacggtagaa aacaaaatcg agcgggcgac
     2281 caaacgcccg acggcgctga tcatcggtaa aacgcacctg acgcagcgac atttcccgcg
     2341 caaaacgata taacgcgttc atccttctac ggctccaggc attgaaatct cccgccataa
     2401 tgaccgggcc gctgtggtga gctatctgat caccaatagg aagtaactgc ttactataaa
     2461 catccacgcc cagactgaag ttgacggcgt gtatattaac caccatcaac aggcgggtgt
     2521 caggtaatgg atagaccgtc accagtgccg acttcgccag acgcaaaatg ggttctcgtt
     2581 cacgtaacgg gcagcaatac actggatgtg ccgccgaaag ggtcattacg ccagaaggat
     2641 gttgtggcag cacgaaagcg ggtacctgat cggcggcaag atagttagca gtcgcaaact
     2701 gtaccaactc tggcgttgtt tgcgcttcct gcaataacac cagatgtgcg tctttgccgt
     2761 aattctttaa taccgacaac cattcagcgc gttgctgttt gtatatgttc cacaccagga
     2821 tccgaatacg ctcttcggta cttaacggtt ctccgggtgg taatgcctgg ccgatgctcg
     2881 caaaagaccc cggcggtaag atcctctccg caggttgtcc ggcaacatag cgcatggcat
     2941 aggtgttttt tcgcactttt gactttgaaa cctctattac gtcaaccctt ccgcactcgg
     3001 gagggttcta ctgttatagg gattttagct cactctttca acgagcaatt gtatattgtt
     3061 atgtaagcaa gtgcttacac cggtcagaat tttgctcatt attgagaaca ttaaatcgga
     3121 tgacatatca cgcatcctga tggtttaatg acaaataaat tcaaaatatc tcatctattt
     3181 tgcaacagga gcaaaaatga aagccacgtc ggaagaactc gccatttttg tttcggtcgt
     3241 cgaaagcggc agctttagcc gggcagcgga acaattaggg caagcaaact cagcggtaag
     3301 ccgggcggtg aaaaagctgg agatgaaact tggcgttagc ctgcttaatc ggaccacgcg
     3361 gcaacttagc ctgacggaag aaggcgagcg ttattttcgt cgcgtacagt caattttgca
     3421 ggagatggca gcggcagaat cagaaattat ggagacgcgt aatacaccgc gtggactgtt
     3481 gcgcatcgat gccgcaactc cagtggtgct gcactttctg atgccgttga ttaagccttt
     3541 ccgtgaacgc tatccggaag tcactttatc gctagtctcc tccgaaacga ttattaattt
     3601 gatcgaaaga aaagtggatg tcgcgatacg cgctggtgcg ttaacggatt ccagcttacg
     3661 tgccaggccg ttatttaaca gttatcgaaa aattatcgcc tcccccgatt atatttcccg
     3721 ctacgggaag ccagaaacga tcgacgattt aaagcaacat gtttgcctgg gattcactga
     3781 acccgcttcc ctcaatacct ggccgatagc ctgtagcgat ggacaattac atgaggtgaa
     3841 gtacggtttg tcatccaata gtggggaaac actgaaacag ctttgcctga gtggcaacgg
     3901 gattgcgtgt ttgtccgact atatgatcga cagagaaatc gctcgcggag aattggtgga
     3961 gttaatggca gataaagtgt tgccagtgga aatgccattc agtgcagtct attacagcga
     4021 ccgtgcggta agtacgcgca tccgggcttt tatcgatttc cttagcgagc atgtaaaaac
     4081 agctcccgga ggagctgtca gagaggctta atcccattca ggagccagac cttccgggct
     4141 aaccaggcgg tcgttgcaat ccagtgcggc gatcgctttt ttatcttcgg catcaagctg
     4201 taaattttgt gccttaagat tactttccag gtttttacgt ttagtagaag aaggaattac
     4261 tgagtaacct tcccccatag cccacgccag aatcacttgt gccggagtcg cattgtgttt
     4321 agctgcgata cgagcaataa cctcatcttt cagggcctta ccatacgcca gcgtcatata
     4381 ggaagtaata tggatgccgt gctgtttagc ccaggcaacc actttacggt tttgcagata
     4441 aggagagagt tcaatctggt tagtagcgat gttttcagcg ccaacagcag caatcgcctt
     4501 ttccatcaat gggatcgtga agttggaaat accgatctca cgcgtcaacc cttctttttt
     4561 ggcttccagc agcgcctgca taaactcttc aacagagact tcatcgtttg gtgacggcca
     4621 gtggattaga gtcagatcaa cataatcagt acgcaatttt tgcaggctct ctttcagact
     4681 cgggatcaat ttgtctttgc tgagattttc aatccagatt ttagtggtga tgtagagttc
     4741 atgacgtgac acgccacttt ctgcaatcgc ctgacctacg gcagcttcgt tatcatagat
     4801 ttgtgcggta tcaattgcgc gataaccaag ttcaagcgcc gttttcacag atgaaataac
     4861 aacgtcgtct ttcagacgga aagtacctaa accaaatgca gggatagcca taatatgcct
     4921 cttttaatgc tattttcgtt aactgaggtg attatgggct tcagactgtt gcggaaaaag
     4981 agcgtaaaac gcagaggatt tttgcgattt tagcaaaaat agaaatgtag acgaaaaaaa
     5041 acctcgagcg ataactcgag gcttcttaat a
//
LOCUS       DAZKFU020000295         5054 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZKFU020000295 DAZKFU020000000
VERSION     DAZKFU020000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.297"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(26..250)
                     /gene="cspD"
                     /locus_tag="ACHOQQ_RS21680"
                     /old_locus_tag="pgaptmp_004336"
     CDS             complement(26..250)
                     /gene="cspD"
                     /locus_tag="ACHOQQ_RS21680"
                     /old_locus_tag="pgaptmp_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGU4472506.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQFVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            400..474
                     /gene="yljB"
                     /locus_tag="ACHOQQ_RS21685"
                     /old_locus_tag="pgaptmp_004337"
     CDS             400..474
                     /gene="yljB"
                     /locus_tag="ACHOQQ_RS21685"
                     /old_locus_tag="pgaptmp_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGU4472507.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            573..893
                     /gene="clpS"
                     /locus_tag="ACHOQQ_RS21690"
                     /old_locus_tag="pgaptmp_004338"
     CDS             573..893
                     /gene="clpS"
                     /locus_tag="ACHOQQ_RS21690"
                     /old_locus_tag="pgaptmp_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGU4472508.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            924..3200
                     /gene="clpA"
                     /locus_tag="ACHOQQ_RS21695"
                     /old_locus_tag="pgaptmp_004339"
     CDS             924..3200
                     /gene="clpA"
                     /locus_tag="ACHOQQ_RS21695"
                     /old_locus_tag="pgaptmp_004339"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGU4472509.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            3779..4987
                     /locus_tag="ACHOQQ_RS21700"
                     /old_locus_tag="pgaptmp_004340"
     CDS             3779..4987
                     /locus_tag="ACHOQQ_RS21700"
                     /old_locus_tag="pgaptmp_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4472510.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYVFPQLGAKTLDTITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQSRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHVLENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLDQRIPMMQAWADY
                     VMSQIMENQR"
BASE COUNT         1348 a         1097 c         1388 g         1221 t
ORIGIN      
        1 ggatgtacac aatgagacag aagagctatg cgactgccgc ttctacttcg acgggcacaa
       61 taacactggc gtgattgcct tttggcccct ggtggacatc aaactgaacg aattgtccag
      121 cttttagcgt tctgtaacca tccatctgaa tggtggaata atgagcgaaa atatcttcgc
      181 cgccgccttc agggcagatg aaaccaaacc ctttggcatt gttgaaccac ttaacagtac
      241 ccttttccat gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga ataaaccggg
      301 gtcagagagg gggctgttca aaacctcgcc aactctagaa atacaattta gagaattagg
      361 gcgagccgtc aagcatttga caggggacaa ggggcaggta tgaatcaaaa atttgaagca
      421 gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag atgatagtta
      481 tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg
      541 ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact
      601 ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag
      661 tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat
      721 tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga
      781 aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca
      841 agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg
      901 cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg
      961 ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg
     1021 cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg
     1081 cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg
     1141 aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct
     1201 tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct
     1261 ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg
     1321 atgtggtgaa ctttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc
     1381 ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca
     1441 cgacgaacct gaatcagctt gcgcgtgtgg gcggaatcga cccactgatt ggtcgtgaga
     1501 aggagcttga gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctgg
     1561 tgggggaatc tggtgttggt aaaactgcga ttgcggaagg tcttgcctgg cgaattgttc
     1621 agggtgatgt gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc
     1681 tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc
     1741 agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg
     1801 cgggtgcagc gtctggtggt caggtcgatg cggctaacct aatcaaaccg ttgctctcca
     1861 gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga
     1921 aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcgatcgaag
     1981 aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac gacgtgcgtt
     2041 ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatt aacgatcgtc
     2101 atctgccgga taaagcgatt gatgttatcg acgaagcggg cgctcgcgca cgcctgatgc
     2161 cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta
     2221 ttgcacgcat tccagagaag agtgtttctc agagtgatcg tgataccctg aaaaacctcg
     2281 gcgatcgcct gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag
     2341 ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt tcgttcctgt
     2401 ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg aaagcgttgg
     2461 gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc
     2521 tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg accgatgcgg
     2581 taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg catccagacg
     2641 tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac aacggacgca
     2701 aagcggactt ccgtaacgtg gtgctggtga tgaccactaa cgccggggta cgggaaactg
     2761 agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca
     2821 agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg ttcgatcatc
     2881 tgtcaactga cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc
     2941 tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga
     3001 aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg tgtcatccag gacaacctga
     3061 aaaaaccgct tgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg
     3121 tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca
     3181 aggcggaagc agcgcattaa tctgattgtc aggtaggttg gtgaagtccg taatctcgtc
     3241 aggggttacg gactttttat ttatggggtg gaacggtgaa aaccctattt ttggaggtga
     3301 aggtaagttg ttgataatta gtgctgctgg aaggtaagga taaaaaaggg tgctgcagga
     3361 gaatgggatg gttttgcttt attaacaacg ggctaaacgt gtagtatttg agttcactgc
     3421 cgtacaggca gcttagaaaa acctaccgtc ttggctagcg gttgcagcga acgttcactg
     3481 ccgtacaggc agcttagaaa ttcactggta acatactcca cccgcccacc atgttcactg
     3541 ccgtacaggc agataaaatg cgaaaaaaaa agctcgcact ttcgtacgag ctcttcttta
     3601 aatatggcgg tgagggggac cgcctgaaat aaatctgact taatccggga gtgttatata
     3661 acttcattat aaacaatgca ttacaatttt tgctgtccga tgttatctaa tgtattctta
     3721 gtgaagtgag agactaatgg catactggat ggcataccag agtaacagga attagcgtat
     3781 ggcagtattg acggatacta aagcaaggca tatcaaacct gatgacaaac cattgcccca
     3841 tgggggaatt acaggactga ctcttcatcc ttcttcagta aaggggcggg ggaaatgggt
     3901 ttttcgttat gtaagtccgg tgacacaaaa aaggcgtaat gctggattgg gtacttaccc
     3961 tgaggtcagt attgctgaag ctgcacgtac tgcccggata atgcgagagc aacttgctgc
     4021 aggtgatgat cctctggaga ttaaaaaggc tgaagctgaa aaagttgtta tcccaacatt
     4081 tgccgatgca gcccggcgtg tacatgcaga attgtcaccg ggatgggaaa acccaaagca
     4141 tgtcaggcag tggttgtcga cgcttgaaaa ttacgtgttt cctcaactgg gagcaaaaac
     4201 gctggatacg attacggctg cggacgtggc agagacactg cgtccagtct ggttgacctt
     4261 atcagaaacg gcaagccggg ttaaacagcg cattcatgtt gttatgcagt ggggctgggc
     4321 gcatggtttt tgtgtggcga atcctgttga tgtggttgat catttgcttc cacagcaatc
     4381 aagaggacgt gatgaacacc agccggcaat gccctggagg cagttaccgc tttttgtggc
     4441 gaccagtgtg tatacagatg aaccttataa tgttacccgg gcactgttat taatggtgat
     4501 actgacagca acccgctcgg gcgaagcaag gggaatgcgc tgggctgaaa ttgattttca
     4561 taagcggata tggacgatac ccgcagaaag aatgaaagcc aggatacagc atcgtgttcc
     4621 tttatcccga caggccattc acgttctgga aaatatacgt ggtctgcatg acgaactggt
     4681 gtttccttct cccagaaagc agcagatcct ttcagatatg gtgttgacga gttttctacg
     4741 taaaaagaag gccatcagtg atatacccgg acgagtggct acagcacatg gttttcgttc
     4801 aacattcagg gactggtgta gcgaacaggg atattcgcgg gatttggcgg aaagggcgct
     4861 tgcccacacg ctgaaaaata aggttgaggc ggcatatcac cggactgatc tgctggatca
     4921 gcgtataccg atgatgcagg catgggcgga ttatgtgatg tctcagatta tggaaaacca
     4981 gcgatgaaat cacatctcac agatttgcag atgtcgtctg taatcacgaa aaatatactt
     5041 attgagttat aatg
//
LOCUS       DAZKFU020000296         5005 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZKFU020000296 DAZKFU020000000
VERSION     DAZKFU020000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.298"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            143..724
                     /locus_tag="ACHOQQ_RS21705"
                     /old_locus_tag="pgaptmp_004341"
     CDS             143..724
                     /locus_tag="ACHOQQ_RS21705"
                     /old_locus_tag="pgaptmp_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4472511.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALELISQGES
                     LGSVTEKTGISRSTYFRLKRTIKNDAKIATFYK"
     gene            complement(780..884)
                     /locus_tag="ACHOQQ_RS21710"
                     /old_locus_tag="pgaptmp_004342"
                     /pseudo
     CDS             complement(780..884)
                     /locus_tag="ACHOQQ_RS21710"
                     /old_locus_tag="pgaptmp_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            complement(1142..3541)
                     /gene="dosP"
                     /locus_tag="ACHOQQ_RS21715"
                     /old_locus_tag="pgaptmp_004343"
                     /gene_synonym="pdeO"
     CDS             complement(1142..3541)
                     /gene="dosP"
                     /locus_tag="ACHOQQ_RS21715"
                     /old_locus_tag="pgaptmp_004343"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HGU4472512.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(3566..4948)
                     /gene="dosC"
                     /locus_tag="ACHOQQ_RS21720"
                     /old_locus_tag="pgaptmp_004344"
     CDS             complement(3566..4948)
                     /gene="dosC"
                     /locus_tag="ACHOQQ_RS21720"
                     /old_locus_tag="pgaptmp_004344"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGU4472513.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
BASE COUNT         1331 a         1177 c         1182 g         1315 t
ORIGIN      
        1 gctaaatatt tgttaaatat ggctagtatc aaatattaag atatctgata cctgtctaaa
       61 acagcataaa agcaggtttc aaaaatcgta ttttgtacat tatgatacca tgcagtgtca
      121 aaaacgataa gtgagactaa aaatggcgtt attggggtat gcacgggtat caaccagtca
      181 tcagaagcta acacatcaaa tctcggagct aaaaaatgcc ggggtacggg aagatcgtat
      241 ttttacggat atgatgactg gcgcaactga tgaacgggaa ggactacagc ggttgttggc
      301 ccgtgcagag aaagacgacg taatcatctg tacaaaaatg gaccgactgg ggcgtaacac
      361 tgctgacatg attcgtgtgg ttgatagctg ttacaaaaag gggatcgcca tccggttcct
      421 ggagaacggt ctcagtacag aagggacaat gggcaaaatg gttatccaaa tccttgctgc
      481 tgtcgctgaa gcggaacgtg agcgtattct ggagcggaca aacgacggaa gaatagcggc
      541 acttgccgca ggcgtaaaat ttggcaggaa gaagcatcca cgaacgccta cagcgttgga
      601 gcttatatca cagggagaat ctctgggcag tgtgacagaa aaaacaggta tctctcgatc
      661 gacgtacttc aggttaaaaa gaacaataaa aaatgatgct aaaattgcaa cgttttataa
      721 ataaattcaa ttaattacct aagcaaggtg agacttttag aggaaatggg cgcgaacccc
      781 caaatagctt tacctgtgat gttatcagcg caggcgtatt tcaattcgat ctccagatca
      841 gggaagatca cggctaaatc aaccagttcg gtggtatccg acataaacgg ttcctgtata
      901 agacaaaaat tgctgcgctt tccgcttatg cagatctcat gccatgccgg gataagcgcc
      961 agaatgctgg cttaaagtta tattttttaa cttttgatca acatttgtgc agcgtagtgc
     1021 agttttggtg caagagggga agttaaggaa ggaatctccc ggaatcgtag ctgaaatcac
     1081 agtatttaag tgacagtgtc acgttaaatg aaaacccgcg agtgcgggcg agaggaattt
     1141 gtcagatttt cagcggtaac acgctgctca tccagcctgg aatttcttcg gcgggtaggg
     1201 ggcgggaaaa gaaatatccc tgaataactc gacagtggat cttacgtagc atctcaaatt
     1261 gctctttggt ttcgacgcct tccgccacga cggttaaatt gaggctttgc ccaatgctgg
     1321 taatggcttc aagtaaggca aggatgcgtt tttccgtcag acaacgatcg acaaaacttt
     1381 tgtcaatttt gatttccgtt accggaagac tgactaagcg ggataatccg gaaaagcccg
     1441 tgccaaaatc atctaccgat aaacccacgc ccatatcacg caggatctga atgcgcttaa
     1501 agatttcggt atcgtgttcc atcatcatgc tttccgtgat ttccaccgtc agctggtggc
     1561 cgtcaatacc ccaggcttgc attgcatcag acacctgatt aggcagttga ttactgcgaa
     1621 agtgcagcgc cgacaagttc acggataacg ccgggatatg aatattctgg ctacgccatt
     1681 ctgctaactg acggcaagct tccgcgatga cccagcgccc aatattttcg atttcaccaa
     1741 tctcttctgc gagaggaata aaccgtgaag ggggcacatg accatgctgg ggatcgtgcc
     1801 agcgagcaag ggcttcgatg ccgtacagtt cacccgtttc tgcgaagatt tgcggctggt
     1861 aaaccagttt cagttggtta ttgctaatcg cctctttcag cgctgcgcct aaaaccaaac
     1921 gctcttttac catttcgttc atcgccgggc tgaagaactg ccagccgtta ccgccattct
     1981 tgcgaatata atccattgca ttgtgagcag tggagagcag gtaatcgcgg tttttaccca
     2041 cgtcgtagct gatgccaata ctcaaggtaa gcgggaaggg tttatcgtca atcattatcg
     2101 gcttgctgac cacattccgt agctcatcgg cgatttgggt aatgttactg acgtcgtttt
     2161 ccaggctcac gaggacaaac tgcgtacctt cgatacgaca gagatactga tccggtttga
     2221 gtttttcacg aaagcgattg accacttcca gcaatgcctg atcggcccac gcatagccaa
     2281 ggctatcaat cacatcctga atatggtcaa caccgatgag atacaccacc ggagagacgg
     2341 ctttgtcgac caggtcatcg aggtaattgt gcaggttatt gcgatttggc agaccggtca
     2401 tcggatcaaa ttggatgagt tgttcaatat gctgacggct tttttcctgt tccagcgcca
     2461 gcgcggccat atgctggctg atatctgcca cgcgttcgat aaaggcgctg gtttctgctc
     2521 ctgacgaggt tttaatttgc aggatccccg caggcgcgcc atcacgctga cgaatggtcg
     2581 ctgaccagct ttgcgcattt tgaacttctg caccgtggga agatgacgcc cagtgtatcg
     2641 gcatcccgtt gcgcagtgcg aacagcgaaa catgcgattc gttgagaaca gattcgatat
     2701 tacgacaaat gatttccccc atttcatgaa atggcgggct gctgcacatg gcggcgagaa
     2761 tattgccttc aagctggcgg atttgccgtt cttcggtgat atccgagaag gtcattacca
     2821 ggttctgcag atgcgcgagc acgtcataaa ccgggctgat agaagcttta atccagattt
     2881 tttcaccggt gcgcgtcaac agcagaaatt cgtcctgatc gcgggcggtt ttccatagca
     2941 actgttgtaa acgaatgcgg ttatcggcag ggaattcagg aatgttcagg agtgtatcgg
     3001 gctgcatacc gctggcttcg ctaatgcagt aaccaaacat ttcggtaaat gcgcgattgc
     3061 actgcacaat atggcgttcc ggatcgagga caatcaccgg tcggtcgaga tggtcaacgg
     3121 caataatcaa ttgcctggtc tgttcttttt gcgccatttc tacgctggca tcccgtacca
     3181 gcgccaggta ataaactttc ccctcggcgc tcacttttga tagcgcaaaa cgggtccaga
     3241 ttttactgcc gtcttttttc tccagctgca gctcccgact catcccctcg acgcgcgctt
     3301 taccgccttc acggttatga cgaatgtatt caggatgcgc aggacgcaaa tcccgcggaa
     3361 tcagcatatc aatgttattg ccaatgactt cttcacgttt gtacccccag agcttctctg
     3421 cggcggggtt gaaaaacatc acttcatcat tttcgttaat taacaccgca cccatcatat
     3481 tttgctcaag ggcggggaaa aaaatgccat cggcggcatt atccgcatcg gttagcttca
     3541 tgattacctc tgcatcctgg cgcatctaaa gactggcttt ccagagttca acacggtttc
     3601 tacctcgtct tttggcgata tacagagctt catcggctat ttgaatgagg cgctcatagt
     3661 caggatgacc attaaacatg gcggcaccga tggaaagtga gagggcaata tcttcgccgt
     3721 ttgcggcttt cagtttggtt ttctccaccc gactgcgaat acgttctgcg gtacgtaacg
     3781 tttcgttttc agaagcttca gtcaaaacaa tgataaattc atcgcccccg tagcggaaaa
     3841 cataatcact actgcggacg ttgtcataaa aggcctgaga gactttacgc agaatttcat
     3901 cgccagtgtt atggccccac gtatcgttga tctctttgaa tttatcaacg tcaataatca
     3961 gcactgacag cggtgtaccg gtccggttgg catgggcaat ttcgcgtttg aagatagtcg
     4021 gcaggaaacg gcggttaagt aatttcgtca gtacatccat accgacttcg tggcgcgata
     4081 cttcttcaaa caattcacgc agcaaggtaa taatttgcga tacggtattt cttatctgta
     4141 ataaaaattt cacccgcaga cttctgttat tcaaattcct ggtgttacgc atggtttgat
     4201 tgaaaatacc gtcgaaatcc tgaatcagac gagagatatg gcccacttca gcaatgccgc
     4261 tgaaataatg tcgaccttta tggttaaacc acaggccaaa atcagcctgg cttaaaggca
     4321 aactactgcc taaatcagaa tccagcagga ttttatagat gatatctatt tcccatgaaa
     4381 gtattgaggc tatttgccgt tctttttctt cttcggcgtt ttccagtaac gagaagatac
     4441 gatagttttc atcttctttt gaggcactac tgtcactaaa ggtaaacgcg cgggtcatca
     4501 cttccatagc gatatcaata ctgttaatcg agaaatggta gacctgaagt ttttctgcgg
     4561 cagaataatc cgaagagaag attaccggat agaggatctt tttcagcacc cgaaacccca
     4621 tctcgacaat ttctaccgga attcctatgc gggcatgcac ttccgcgacg gtatgctgga
     4681 tttgtattag cctttcgaca tcgtcaacct gggcagaaag cacgttaata atccagcgtt
     4741 ccatcgcact tttcaactgc cgctcaactt gttcattact caagaattct tcggcatgcg
     4801 ggtcgatgcg gacaattcga taaaactcaa tactcagata atgagcatgc gcaacggcaa
     4861 tttccgcggc tttagctcga atgggcggat ctgcctgttc gacaagtccg gtccactcat
     4921 ctttcattct tttaaaatac atctccataa ttcacaccct tataaggctg ggaaatcaga
     4981 cggaatcaaa acgcaacgtg cgaga
//
LOCUS       DAZKFU020000297         4949 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZKFU020000297 DAZKFU020000000
VERSION     DAZKFU020000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.299"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            152..949
                     /gene="gpM"
                     /locus_tag="ACHOQQ_RS21725"
                     /old_locus_tag="pgaptmp_004345"
     CDS             152..949
                     /gene="gpM"
                     /locus_tag="ACHOQQ_RS21725"
                     /old_locus_tag="pgaptmp_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HGU4472514.1"
                     /translation="MLTPAQRYVQQAVARNRVTMNARETQRERTAHEQVLHQLRLAQM
                     QLKGIQSNVARAELKKHLLPDFEGWIEGTLAGDSGRQDEVITTMMVWAIDCRDYPLAL
                     RIGEYVIRHGLALPDNFGRDAATVLAEEITDAVLTQATTDSEADLSVYTAMLDELNHL
                     VSEQDMPDQVRSKLHKARAFSRRASSSPDDLRLSLSLLREAMRINPASGVKREITTLE
                     RAVRKLADGNQVVPASTTGRKTTTGGKKSSAKKKSAASQKKAARKTN"
     gene            1054..1548
                     /locus_tag="ACHOQQ_RS21730"
                     /old_locus_tag="pgaptmp_004346"
     CDS             1054..1548
                     /locus_tag="ACHOQQ_RS21730"
                     /old_locus_tag="pgaptmp_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HGU4472515.1"
                     /translation="MSLIARPRVNSADTDTTDTDDGDAMVSAGDFWPAISLHEIRLAS
                     RIPGRTTTTRLLHAATEAVAHVIAELELWKRERMAEGAASLADVPATQVNGESVHVHR
                     YRRAVFALTRAFVLERTRDVDTTDKGDRRADALDMQVEDLWRDARWAISDIRGVTRIY
                     AELV"
     gene            1548..1748
                     /locus_tag="ACHOQQ_RS21735"
                     /old_locus_tag="pgaptmp_004347"
     CDS             1548..1748
                     /locus_tag="ACHOQQ_RS21735"
                     /old_locus_tag="pgaptmp_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HGU4472516.1"
                     /translation="MKVMALDGDTVDLLCQRHYGTTQGVTEAVLAANPGISAVMFLHA
                     GQEVELPELQGQTRREMIQLWG"
     gene            1751..2035
                     /locus_tag="ACHOQQ_RS21740"
                     /old_locus_tag="pgaptmp_004348"
     CDS             1751..2035
                     /locus_tag="ACHOQQ_RS21740"
                     /old_locus_tag="pgaptmp_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4472517.1"
                     /translation="MFQRLCDHITFWASVTTAGIGVMTLSEKIAVAGFVLGTLSLLRA
                     WLHRRRMEIAQARRNELIEKILMQSEGRELNDAERRAMMLLKQDEHDGRT"
     gene            2022..2564
                     /locus_tag="ACHOQQ_RS21745"
                     /old_locus_tag="pgaptmp_004349"
     CDS             2022..2564
                     /locus_tag="ACHOQQ_RS21745"
                     /old_locus_tag="pgaptmp_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4472518.1"
                     /translation="MAGPKRYAVAAIVALGISLAPGELRTSREAQIKIATWEECRARP
                     YRDLVGVGTVGCGSTGNVENRLYTQEEVAGRWINDMRRAENCITQNFRGQQMPQSAFE
                     AMTDAAFNLGCRNLMWFKNKNGTPQRTTIWKHAQARQWRLMCYRLTDFVNSGGTRTQG
                     LVNRRNDFKNWCLKDAGAQP"
     gene            2561..2986
                     /locus_tag="ACHOQQ_RS21750"
                     /old_locus_tag="pgaptmp_004350"
     CDS             2561..2986
                     /locus_tag="ACHOQQ_RS21750"
                     /old_locus_tag="pgaptmp_004350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HGU4472519.1"
                     /translation="MKLNIILCALLLTISLQAMVVIRAYRSGQESTRYEALEKAVKES
                     NTTVRELLKATAETQSALAAIRREDQERAARGEERRETMRKNLQADECARTSPPADVA
                     DSLQQHASAAENRRHLRERAGKSDQTDAGTGAAATHDMG"
     gene            2973..3104
                     /locus_tag="ACHOQQ_RS21755"
                     /old_locus_tag="pgaptmp_004351"
     CDS             2973..3104
                     /locus_tag="ACHOQQ_RS21755"
                     /old_locus_tag="pgaptmp_004351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472520.1"
                     /translation="MIWADVADWGDRLVDALDSCNADKAAIAGLERQRTARMNTHEK"
     gene            3115..>4949
                     /locus_tag="ACHOQQ_RS21760"
                     /old_locus_tag="pgaptmp_004352"
     CDS             3115..>4949
                     /locus_tag="ACHOQQ_RS21760"
                     /old_locus_tag="pgaptmp_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4472521.1"
                     /translation="MVPKHYQLVTAMTNKELAEKLAPMLREGWGLLGGPVSASEPGAP
                     FYLMQALVTDRDLPSAIPVPEGEEKPVPGTTDGYNTGHTTEPEFYYVVPLAGQSNGMA
                     YGEGLPLPDTFDSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVQDMSSLNHP
                     NADLSKGQYGCVGQGLHIAKKLLAFIPKNAGILLVPCCRGGSAFTSGAEGAFSESSGA
                     TAESSLWGVGKPLYNDLLTRTKAALDKNPKNVLLSVVWMLGEFDLKSATYAQHPQLFL
                     NMVNQFRADLSAYAAQCTGGNASLVPWICGDTTYIWKQQYTSQYPVVYGAYQNKEAQK
                     IYFVPLMTDEKGANTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRG
                     IISDRLATAILMHAGKTVAFLKGAPAPFSVVAVPPATAGSDSTTTESPQPSTPAPQPG
                     AGTQSSPARTTAVAYRADAQEGKLKEQGWTGADGKASVVNDSAAESGKAMRVEKVQGQ
                     QTAWKIAHDVPASKAAELLQKGGEIAFRFKIPEGVQLAANRFAMALYWPVSQLPENVV
                     PEGSTGSNMLAAFFIQSDSANLNLMQHKQQNAKLGTFGAFNHNWH"
BASE COUNT         1232 a         1355 c         1459 g          903 t
ORIGIN      
        1 tgctacgtgg tggaagacta cggaaaatgc gcgctgattg aggggatcac cttcgcaggt
       61 gaagatccgg acaacaacgc cgaaagcgga cagccgcaac ccgaagcata acccggacgg
      121 gcgcgccgcg cccgttaaca ggagctgaga aatgctgaca ccggcacaac gctatgtgca
      181 acaggcggtg gcccgcaacc gggtgaccat gaacgcccgg gaaacacaac gggaaagaac
      241 cgcacatgaa caggtccttc accagttaag gctggcacag atgcagctga agggtattca
      301 gtcaaacgtc gccagggcag agcttaaaaa acatctgttg cctgactttg aagggtggat
      361 tgaaggcacc ctggcaggag acagcggacg ccaggatgag gtcatcacca ccatgatggt
      421 gtgggccatt gactgccgtg actacccgct ggcactgcgt atcggggagt acgtgatccg
      481 ccatggtctg gcgctgccgg ataactttgg ccgtgacgcc gccacggtac tggctgaaga
      541 gattactgac gccgttctga cgcaggccac aacggacagc gaggcggatt tgtctgtgta
      601 caccgccatg cttgatgagc tgaaccacct ggtcagtgag caggatatgc cggaccaggt
      661 caggtcaaaa ctgcacaagg cacgcgcatt ttcccgccgc gcctcctcct ctcccgatga
      721 tctgcgtctg tcgctgagcc tgttacgtga agccatgcgg atcaatccgg cttcgggagt
      781 gaagcgtgaa attaccacgc tggagcgcgc ggtcaggaaa ctggcagacg gaaatcaggt
      841 ggtgcctgca tccacaacgg gcaggaaaac caccaccggc gggaaaaaga gcagcgcaaa
      901 aaagaaaagc gctgccagcc agaaaaaagc cgcgcgcaaa acaaactgac ttaacgaacc
      961 gcacctcgtc ggcaggcggc gcaaccggtg atctgaacag gcttcgcgtt ctttttaccg
     1021 gttgcccacc gcctgatttt tacggagaca ggcatgagtc tgatagccag accccgcgtg
     1081 aacagcgctg acacagatac caccgacacc gatgacggcg acgccatggt gagcgcgggt
     1141 gacttctggc cggcgatctc actgcatgaa attcgcctgg cttcccgcat tccggggagg
     1201 acaacaacaa cccgtctgtt acatgcagca acggaagcgg tcgcccatgt gatcgccgaa
     1261 ctggagctct ggaaacgtga gcggatggct gaaggcgcgg cctcactggc tgacgttccg
     1321 gccacgcagg tgaacggaga gagtgtacac gtccaccgct accgcagggc cgtcttcgca
     1381 ctcacccgcg cgtttgtact ggagcgcacc cgggatgtgg ataccaccga taaaggcgac
     1441 cgcagggctg acgcgctgga catgcaggtt gaggatttgt ggcgggatgc ccgctgggca
     1501 atatcagaca tacgcggtgt cacccgcatt tatgcggagc tggtctgatg aaagtaatgg
     1561 ctctggacgg tgacaccgtc gatttactct gtcagcgcca ttacggcacc acgcaggggg
     1621 tgactgaggc ggtactggcc gccaatccgg ggatttcagc cgtcatgttt ctgcatgccg
     1681 gacaggaagt ggagctgccg gagctgcagg ggcagacccg gcgtgaaatg atccagttgt
     1741 gggggtgaga atgtttcagc ggctttgtga tcacatcacg ttctgggcgt cagtgacgac
     1801 agccggtatc ggcgtgatga cgctgagcga aaaaattgcc gtggccggtt ttgtgctggg
     1861 gacgctgtca ctgcttcgtg cctggcttca ccgccgcagg atggagatcg cccaggcaag
     1921 gcgtaacgaa ctgattgaaa aaatccttat gcagtcagaa ggaagggagc tgaacgacgc
     1981 agagcgccgg gcaatgatgc ttctgaagca ggatgagcac gatggccgga cctaagcgtt
     2041 acgccgtggc cgccattgtg gcgctgggga tctccctggc tcccggagaa ctgagaacct
     2101 cccgcgaggc acaaatcaaa attgccacat gggaggagtg ccgggcccga ccctaccgtg
     2161 atctggtggg ggtcggtacg gtcggatgcg gatccaccgg taacgtggaa aaccgcctgt
     2221 acacacagga ggaagtggcc gggcggtgga tcaacgatat gcgccgcgct gagaactgca
     2281 tcacacagaa tttcaggggg cagcagatgc cgcagtctgc attcgaagcc atgacggacg
     2341 ccgcctttaa cctgggctgc cggaatctga tgtggtttaa aaacaaaaac ggcacgccgc
     2401 aacgcaccac catctggaaa cacgcgcagg cacggcaatg gcgcctcatg tgttaccggc
     2461 tgacagattt tgtgaacagt ggcggaacgc gcacgcaggg actggttaac cgccggaatg
     2521 attttaagaa ctggtgcctg aaagacgcgg gagcgcagcc atgaaactga acattatcct
     2581 ttgtgcgctg ctgctgacca tttcgctgca ggcaatggtg gtcatccggg cctatcgctc
     2641 aggtcaggag agcacccgtt atgaagcgct ggaaaaagcc gtgaaagaga gcaatacgac
     2701 cgtccgggag ttgcttaagg ccacggcgga gacacagtca gcgctggccg ctatccggcg
     2761 cgaagatcag gagcgtgcag ccagagggga agaacgacgt gaaacgatgc ggaaaaactt
     2821 acaggcggat gagtgcgcac gcacttctcc ccctgctgat gttgctgaca gcctgcaaca
     2881 acacgccagc gccgctgaaa accggcgtca tttgcgggag cgtgccggaa agtctgacca
     2941 gacagacgcc ggtaccggtg ctgccgcaac acatgatatg ggctgatgtg gctgactggg
     3001 gagaccgact ggtagatgcc ctggacagtt gtaatgccga taaagccgcc attgccgggc
     3061 tggagcggca gcggacagca cgaatgaaca ctcatgaaaa ataacggagt cactatggta
     3121 ccgaaacatt atcagcttgt gacggcaatg acgaacaagg aacttgccga aaaacttgcg
     3181 cccatgctca gggaaggatg gggacttctt gggggacctg tctcagcatc agagccggga
     3241 gcaccatttt acctgatgca ggcactggtc actgacagag acctgccatc agccatcccg
     3301 gtaccagaag gcgaggaaaa acccgtaccg ggcactaccg acggatacaa cactggacac
     3361 acaactgagc cagagtttta ttatgtggtg ccactggctg gtcagtccaa tggcatggca
     3421 tacggtgagg ggctgccatt accggataca ttcgacagtc ctgatccacg aattaaacag
     3481 ttagcgcgtc gcagtacggt gacaccgggc ggcgctgcat gtacctataa cgatattatt
     3541 ccggcagatc actgtctcca tgatgtacag gatatgagca gccttaatca tccgaacgcc
     3601 gatctgtcaa aaggtcagta tggctgcgtc ggacagggtt tacatattgc caaaaaatta
     3661 ctggcgttta tcccgaaaaa tgccgggatc ctcctggtac cttgctgtcg tggagggtca
     3721 gcgtttactt ccggcgcaga gggggcattc agtgaatctt caggagcaac ggccgaatca
     3781 tccctgtggg gcgtcggcaa accgctgtat aacgatctgc ttacccgtac aaaagccgcg
     3841 ctggataaaa accccaaaaa cgtcctgtta tccgtggtct ggatgctggg agagtttgat
     3901 ctgaaatcag caacatacgc acagcacccg caattattcc tgaacatggt gaatcagttc
     3961 cgcgccgatc tcagcgcata tgctgcacag tgcaccggag gcaacgcatc acttgttccc
     4021 tggatttgcg gcgataccac ctatatctgg aaacaacaat atacctctca gtacccggtg
     4081 gtttacggtg catatcagaa caaagaagcg caaaaaattt attttgtgcc cctgatgacc
     4141 gatgagaaag gtgctaacac gccaacaaac ctgcctgccg aagatcctga cctggcagaa
     4201 gccggttatt acggtgctgc atcccgtagt aacggaaact ggacctcttc agatcgcgcc
     4261 acgcatttca gttcatgggc ccgccgcggc attatttctg accgcctggc aacggcgatc
     4321 ctgatgcatg ccgggaagac cgtcgccttc ctgaaaggtg cacccgcacc gttcagcgtg
     4381 gtcgcagtac ctcccgccac ggccggttca gacagcacca ccacggaaag cccgcagccc
     4441 tccacacctg cacctcagcc gggcgcaggt acgcagtcat caccggcaag aacaacggcc
     4501 gtcgcttatc gcgcagacgc acaggaaggg aaactgaaag aacagggctg gacaggggca
     4561 gatggaaaag cctccgtcgt aaatgactct gctgctgaga gcggtaaagc catgcgagtg
     4621 gaaaaagtcc aggggcaaca aacagcctgg aaaatcgccc acgatgtgcc tgcaagtaaa
     4681 gcagctgaat tacttcagaa aggcggggaa attgcctttc gtttcaaaat cccggaaggt
     4741 gttcagctgg cagcaaaccg cttcgccatg gctctgtact ggccggtttc tcagctacct
     4801 gaaaacgttg ttccggaggg aagcacagga agcaatatgc tggcagcctt cttcatccag
     4861 agcgacagcg caaacctgaa tctgatgcag cacaaacaac agaatgcaaa actgggaacc
     4921 ttcggggcat ttaatcataa ctggcacaa
//
LOCUS       DAZKFU020000298         4947 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZKFU020000298 DAZKFU020000000
VERSION     DAZKFU020000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.300"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..4761
                     /locus_tag="ACHOQQ_RS21765"
                     /old_locus_tag="pgaptmp_004353"
     CDS             <1..4761
                     /locus_tag="ACHOQQ_RS21765"
                     /old_locus_tag="pgaptmp_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HGU4472522.1"
                     /translation="YVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGA
                     RGKGKAAAHYEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFM
                     TEAQRVRRIFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYW
                     STDVEMAARAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRD
                     AFDHLFSTLKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVVRG
                     VMSGIKDSDLKVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRT
                     VREKLRHEIIHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPE
                     VQAGEFLAHMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLG
                     QRVREGYTPREDGVGASSQYSRSGKPDPFKADAYDAKQFASEVRRIAGLQSVSRREML
                     RVGDTPRVLRALGAPDLELVMPASVVHKATRPEIREHFVSVETLADLPQLLTDPVVVM
                     RSKTESDALVSLIQATDTKGNPVIVAIHTHVKGDGFAEINKIASAYGKDKRSALQDQL
                     DTSLLYLNKEKAAEWLHAVGLQLPEANTINGSSGFKILHPEDIRKGPYYSRTSSLTPE
                     DTIASRFVRQMQDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKE
                     RYVQPLAKLLADYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEI
                     MQRVRNSGKQAQYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQNAYRYYVPLKGQ
                     DVDGVVSLPRTGKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNA
                     FLKLVQDNPDKDYWQVFTDDRPDTMRVIAERKDQETGETIREVVERPVAMAMMADRYF
                     TTKKNGKTYYIKLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVS
                     NFIRDVQTAVMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGK
                     GTQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNL
                     VEDANGAVENALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFA
                     NASIQGTANLVRTLGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAG
                     EDDDGVNWYDKVPSHVKERNLVIMKSMFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVA
                     AGDLTASRAAGNVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFM
                     GAQIYQENMPFGTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLK
                     FWIDYISGGTGRFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRM
                     TEVAQYHAELKSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTL
                     SLAQQSAMVKSVERDMKIAVDRFNREYNKKVGVE"
BASE COUNT         1250 a         1122 c         1487 g         1088 t
ORIGIN      
        1 tatgtggaag gtccgcgtcg tcaggctgat ttgaaccggg cttatgactc gctgcatgac
       61 cttgcggaag tactgaatgt accgacaaaa gcgctttccc tgaacggtcg tcttggcctg
      121 gcatttggtg cccgtggtaa gggtaaggcg gcagcacact atgagccggg tgaggtggca
      181 atcaacctga caaaaggtaa cggaccgggt gcgctggcgc acgaatggtt ccattctctg
      241 gataattatt ttggtcgtta tgacgtttcc aatgacggga aaattacgtc aggtggtgac
      301 tttatgacgg aagcacagcg tgtcaggcgc atatttaaag acggcaggta tgttgatgct
      361 gaatatccgg tacgtcagga ggtttacgac gcttttaaag gtgtgattca ggccattaaa
      421 aacagtgaca tgccgcgtcg ttcagcgctt ctcgatgagg tgcgctcaaa accgtactgg
      481 tcaacggatg ttgaaatggc ggcacgtgcc tttgagcgtt atgttcagga taaggcgcgt
      541 atggctggcg tggagaatga ttatctggtc aatatccgta aggcgcctga gcacaacaca
      601 gataacacct gggcttatcc gacgaatgcg gaactggatg gcggtattcg tgatgcattc
      661 gatcacctgt tcagtacgct gaaaacccgt gagacggaca agggagttgc gttttattcc
      721 cgtaagggcg tgacccgcac acctgaaggc aatctcattt cggatgttaa ccgcagtgcg
      781 gaagccaaag gcagcccggt cccgcaggtt gaagcggttg tccgtggcgt gatgagcggc
      841 attaaggaca gtgatctgaa ggtccgtgtg gtgaagtcac agaaagaggc tgaagcgctg
      901 gcgggcgaat tgttcgatgg ttacggcagg gtgcacgcat tctatcgtcc ggataaacga
      961 gaaattgtcc tggtggcgga taacatccct gacgggcgga ccgtacgcga gaagttgcgt
     1021 cacgagatta tccaccatgc catggagcat gttgtcacgc cagcggaata tcagacgatt
     1081 atcaaaaccg tgctgaaaac ccgcgacagt gataacgcca ccatccgtga agcctggcgt
     1141 aaggttgatg cgtcctatgg taaggaatca ccggaagtgc aggcgggtga atttctggcg
     1201 catatggcag agaaacagcc gaataaattt gtggcggcat gggagcgtgt tgttgccctg
     1261 gtcaaagggg tactgcgtcg tacggggtta ctgaaaccga cggaactgaa cgatatcaga
     1321 cttgttcgcg agaccatccg tacgttaggc cagcgtgtgc gggaaggtta cacaccgcgt
     1381 gaggatggag tgggtgcatc gtctcagtac tcccgtagtg gtaaacctga tccgtttaag
     1441 gctgacgcct atgatgcaaa gcagtttgct tcagaagtgc gtcgcattgc cggattacaa
     1501 tcggtttcca gaagggaaat gctcagagtt ggggatacgc ctcgtgtttt gcgcgcattg
     1561 ggggcaccag atcttgaact ggttatgcct gcatctgttg tgcataaggc tacgagacca
     1621 gagatcagag aacactttgt gtcagtggaa acactggctg atttgcctca acttctgaca
     1681 gatccggtag tagttatgcg ttcgaaaact gaatcggatg cgttggtatc tttaattcag
     1741 gccaccgata caaaagggaa tcctgtcatt gttgcaattc atacccatgt gaaaggcgat
     1801 ggatttgcag agataaacaa aattgccagt gcctatggaa aggacaaaag gagtgctttg
     1861 caggatcagc ttgatacatc tttgctgtat cttaataaag aaaaagccgc tgaatggctt
     1921 catgcagtcg ggctccaatt gcccgaggcg aacaccatca acggctcctc tggatttaaa
     1981 atactacatc ctgaggatat tcgcaagggg ccgtattact cccgtaccag cagtctgacg
     2041 ccggaggata caattgcatc gcgttttgtg cgccagatgc aggataaatt ccaggtgctg
     2101 aaagctgttc aggagaatat ccgtaaaact ggcggaaaaa tagacgacag taacaacgct
     2161 tatatggcgg aagaactctt ccacgggaag gcggaaaacg acctgaacgt gatgaaggag
     2221 cgctatgttc agccgctggc taaattactg gcggactata agattgcgca ggccgatctg
     2281 gatgagtacc tctacgcccg tcacgcgccg gaacgtaacg cgcatatcgc gaaaatcaac
     2341 ccgaaaatgc cggacggcgg ttcggggatg accaacgcgg aggcggcgga aatcatgcag
     2401 cgtgtgcgta acagcggcaa acaggcacag tatgaccgtc tggcagggat tgttgacgat
     2461 atgctggccc gtcgccgtga gcttatccgt gaggccggac tggaagagag cggtgtggtg
     2521 gatgcctggc agaacgccta ccgttactac gttcccctga aaggccagga tgttgacggt
     2581 gtggtgtcac tgccccgtac aggtaagggc ttcaccatcg gcggacgtga aagcaggcag
     2641 gccatggggc gtgcatcccg tgcacagtct ccgtccactc aggcgataca ggacctgagc
     2701 gaatcgctga tccgccatcg caaaaacgaa gtgggtaacg ccttcctgaa actggtgcag
     2761 gataatcccg acaaggatta ctggcaggta ttcactgatg acagaccgga taccatgcgt
     2821 gtgattgcag agcgcaagga ccaggaaact ggtgaaacca ttcgcgaagt tgtcgagcgt
     2881 ccggtggcga tggcaatgat ggcagaccgg tacttcacca ccaaaaagaa cggcaaaacg
     2941 tactacatca aactccatga cccgcgcctg atgcgtgcga tgaagaatat gggaccggaa
     3001 accagtaacg ccgtaatccg tacgctgggg aaagttaacc gcttcctggc aacggtgaac
     3061 acgtcgtata acccggaatt cctggtcagt aacttcatcc gtgacgtgca gacggcggtg
     3121 atgaacctga aggcagagca gggaaggagc gacggtaaac tgaaagggct ggataactta
     3181 tctgccctgg ctgtagtcaa agacagccgt tctgcaatgt cagccgtgta cgccagtctg
     3241 cgaggcaaaa ctctcacggg caaaggtaca cagtggcaga aggtgtggaa agagtttgtt
     3301 gaggacggag gcaaaaccgg ctggtttaac atgggtgacc ttgaaggcca gcagaaggaa
     3361 atggatcgcc ttgtctcact ggcgaagggg ggatggaaag gccagagtat cggtgcatgg
     3421 aattcgttcc tgaacctggt cgaggatgcc aacggcgcgg ttgaaaacgc actgcgtctg
     3481 tcagcttata aacacgcccg tgatgccggt ttgtcacgcc agcaggcggc gtctcttgcc
     3541 aaaaacatga cggtgaactt taaccgtcgt ggtgagcagg gggcgctgat gaactcgctg
     3601 tacatgttcg ccaacgccag tattcaggga acggcaaatc tggtgagaac gctcggacat
     3661 cttaatggcg acgggccgtt actggagcgc cttcgctgga agaatctcaa tgtaccgcag
     3721 aaaatcgcgc ttgcagctgt gggagcgggt tatctgcttg gctcgcttaa ccgcagtgtt
     3781 gcgggggagg atgatgacgg ggttaactgg tatgacaagg tgccgtctca tgtgaaagag
     3841 cgtaacctcg tcattatgaa atcgatgttc gggggcaagg ccggagagta ctggagtatt
     3901 cctctgcctt acgggtacaa cgttttcttc ctgctcgggc ataccgctga aggtgtggcg
     3961 gcgggtgacc tgacggcgtc ccgtgctgcc ggtaatgttg ttggtggtgt gcttggtgca
     4021 ttcagcccga ttggcagtga gacgtcggaa acactgtccg gggcattgct gaaaaatgca
     4081 gcgccgacca ttctgcgtcc gtttgcgaac cttgccatga atgaaaactt catgggggcg
     4141 cagatttacc aggagaacat gccgtttggt acaccaaaac ctgacagcca gctgggaaga
     4201 cgttcaacgc cagaagcgta caaggcgttt gcatcctggc tgaatgcgtt ctctggtggc
     4261 agccagtacc gtcccggcgc ggtggatatc acaccggaat cgctgaaatt ctggattgac
     4321 tatatctctg gcgggaccgg gcgcttcatt tctaaaacca cggatgcggc ggtgaaatcg
     4381 ctgaatggta ttgatatacc ggagcagcag gtgcccttcc tggggaaaat ttcaggtgag
     4441 gtgatgccgt atgctgacca gcagaagatg tacgaccgga tgacggaggt cgcgcagtat
     4501 cacgcagagc tgaagagtct gaccggtgca gaaagaacgg cgttcattga cgagaacaac
     4561 ggaaaattgt cgatgaacgg gcttatgcag gatacccgga agagactgaa ggatttgcgt
     4621 aaacagcgtg atgccattta cgccgacagt actctcagtc tggcgcaaca gtcggcgatg
     4681 gtgaaatcgg ttgagcggga tatgaaaatt gccgtggatc ggtttaaccg cgagtacaac
     4741 aaaaaagtgg gagtggagta aagaacatgc cccgtacgga tgtacggggc tgatataaag
     4801 tcacgcaggg aaatattgta gatgaactga acatttaaat tcggataatg ttgtgatctt
     4861 gatcagaaat tacagtgtaa gcagatgtga aatgtcatgc ctgatgaggg taagattttt
     4921 cttatttcgg ttcctacgtt gctctat
//
LOCUS       DAZKFU020000299         4936 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZKFU020000299 DAZKFU020000000
VERSION     DAZKFU020000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.301"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..479)
                     /locus_tag="ACHOQQ_RS21770"
                     /old_locus_tag="pgaptmp_004354"
     CDS             complement(<1..479)
                     /locus_tag="ACHOQQ_RS21770"
                     /old_locus_tag="pgaptmp_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4472523.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQLSALTAMTASFVLATPTETDG
                     "
     gene            complement(479..808)
                     /locus_tag="ACHOQQ_RS21775"
                     /old_locus_tag="pgaptmp_004355"
     CDS             complement(479..808)
                     /locus_tag="ACHOQQ_RS21775"
                     /old_locus_tag="pgaptmp_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4472524.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKRY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(805..3366)
                     /locus_tag="ACHOQQ_RS21780"
                     /old_locus_tag="pgaptmp_004356"
     CDS             complement(805..3366)
                     /locus_tag="ACHOQQ_RS21780"
                     /old_locus_tag="pgaptmp_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4472525.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMTRVRRHFSGLETDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRMLPMQFTDVATQLAGGQNPFLIMLQQGGQVKD
                     SFGGMIPMFRGLAGAISLPMAGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTAERMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDSINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPDTAQEMLIKAETAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQAELNKALRDGKILQADYNTLMASAKKDYEST
                     QKKPKSSGVRVSAGERQEDRAHAALLALETELRTLEKHSGANEKISQQRRDLWKAESQ
                     YAVLKEAATKRQLSGQEKSLLAHEKETLEYKRQLAELGDKVEYQKRLNELAQQAARFE
                     EQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPQALARVTGALKQTWADEDM
                     LRGDWLAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQNWRGFTR
                     SVLSMMTEILLKQAMVGMVGRIGSAIAGAFGGGASASSGTAIEAAAANFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
     gene            complement(3347..3760)
                     /locus_tag="ACHOQQ_RS21785"
                     /old_locus_tag="pgaptmp_004357"
     CDS             complement(3347..3760)
                     /locus_tag="ACHOQQ_RS21785"
                     /old_locus_tag="pgaptmp_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4472526.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRREEAQTERPDEEDMLMQKAAGLAGGVRFGEEGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPAGW"
     gene            complement(3787..4218)
                     /locus_tag="ACHOQQ_RS21790"
                     /old_locus_tag="pgaptmp_004358"
     CDS             complement(3787..4218)
                     /locus_tag="ACHOQQ_RS21790"
                     /old_locus_tag="pgaptmp_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4472527.1"
                     /translation="MFLKTEQFEYNGVSVMLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMSEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNVLTDDDFSVGKSSTAS"
     gene            complement(4235..>4936)
                     /locus_tag="ACHOQQ_RS21795"
                     /old_locus_tag="pgaptmp_004359"
     CDS             complement(4235..>4936)
                     /locus_tag="ACHOQQ_RS21795"
                     /old_locus_tag="pgaptmp_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4472528.1"
                     /translation="LWVYTGKGDAYANPLSDDEWTRLAKIKDLTPGEMTAEPYDDNYL
                     DDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDSSETRAYKIRFPNGTVD
                     VFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGEITPVTGITVTPLTGNVAKG
                     QSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKGVAAGKAQIPVVTGNGE
                     FAAVAEITVTDGAAG"
BASE COUNT          966 a         1678 c         1177 g         1115 t
ORIGIN      
        1 ccgtccgtct ccgtcggtgt cgccagcaca aacgaggccg tcatggccgt cagcgctgat
       61 aactgctcca ccacccaccg gtccgtcagc tcctgctccg ggtctgcctc cggattcccc
      121 gccacaaagt tcaccgcatc aagaaaacgc gcatacaccc ggcggcggac caccgtggca
      181 cccaccaggc tctgcaaatc ctccgccatc ccggtgacaa ggccgaaaag attggacacc
      241 gtcagcgacg ggcgggcact gctgcccttt ccgttcatct caaagccgct gccctcaatc
      301 gggtacgcct catacttccg cccctgccag gtcaccggct cccctttttc attcagctca
      361 ttgcagaaaa aataccgctc accgccctgc accgtcaggt cgatttccca gagcaccacc
      421 cgcggtgact gctctgactt aaccgactcg ttcaggcttt cttcgtgaat atcctgcatc
      481 agttcaccac ctgcttaaac tccgcgctga actcaacgcg caacatcccg acccgcgcag
      541 accacccggc gcaggtcacc tttatctgcc ggtatgcata gggtggcttc cacaaaaatg
      601 ccttccagcc cccgtgctct gccagaaacg cttccagatg ccgggcctcc tcccgggtca
      661 cggaaagcat caccctgtac ctcttcaggt ctgcattcag ccctgccgcc atacgctgtg
      721 agtacccgtc accaaaacgc acttcacgca ccgacggctg cgagttcacc tccatatccg
      781 gcttcacttt ccagcgaaag gttttcatcg cccgctccct gataacatac cgccatcacg
      841 caactgcagc cggagttcat cctgcgcccc cttacgggcc atgtcataca ccgccttcat
      901 cagctgcggc cccgcctgtc cgtttatacc gtcgttctga atcaccacgt gattgttctg
      961 attaaaatta ataccttccg cccgccgcat ctgcgccgga cttccggcac cacccacata
     1021 acccccttcc gcatagccgc gcatcaggcg gtaaagattc cccacaccta tccggctggt
     1081 tgcctccttc gtgaagacaa actccccgcg gtgaactatc cccgcaggct catatttgcc
     1141 gcccgtcccc gtaaatcctc cggtcgcgaa atggaagttc gccgccgcag cctcaatggc
     1201 cgtccccgag gaagcagatg caccaccacc gaaagcacca gcaatggcgc tgccgatacg
     1261 cccgaccatg cccaccatgg cctgtttaag caggatttct gtcatcatgg acagcaccga
     1321 acgggtgaat ccccgccagt tctgttcact gccggtcagc atcgccgcca tattctgtgc
     1381 aataccgtca aaggtctgcg tggccacact tttaacctgc gaaaaactgt ccgtcgcact
     1441 ttccgcccac tccccccagc cggacttcag cccggccagc cagtcaccgc gcagcatgtc
     1501 ttcatccgcc catgtctgtt tcagtgcccc ggtgacccgg gccagcgcct gcggattatc
     1561 gccatacacg tcacgaagac gctgcgcttc agactcccgc tgcgcctgac ggtcagtgag
     1621 accacgggct tttgcgctga tggcggcctg cttcgcgctc tgctgctctt caaaccgtgc
     1681 cgcctgctgt gccagctcat tcagccgttt ctggtattca accttgtcgc ccagctcagc
     1741 cagctgacgt ttgtactcca gcgtctcttt ctcatgggcc agcagggatt tttcctgccc
     1801 ggataactgt cgtttcgtgg cagcctcttt caggaccgca tactgacttt ccgctttcca
     1861 taaatcacgg cgctgctggc tgattttctc attcgcaccg ctgtgcttct ccagcgtccg
     1921 gagctcagtt tcaagcgcca gcagggctgc atgcgcccgg tcttcctgac gctccccggc
     1981 tgacactctg acacctgacg acttcggttt tttctgcgtt gattcataat cctttttcgc
     2041 cgacgccatc agcgtgttgt aatccgcctg caggattttc ccgtctctca gagccttgtt
     2101 cagttctgcc tgacgggcgg tatatttctc cagcggtgtc tgcaggcgtt cgtaagcctt
     2161 ctgcgcctct tcggtatatt tcagccgtga cgcttcggta tcgctctgct gctgcgcatt
     2221 tttgtcctgt tgagtctgct gctcagcctt ctttcgggcg gcttcaagcg caagacgggc
     2281 cttttcacga tcatcccagt aacgcgcccg cgcttcatcg ttaacaaaat aatcatcctt
     2341 gcggagattc cagatgtcgt ctgctttctt aaacgcagtc tctgccttaa tcagcatctc
     2401 ctgcgcggta tcaggacgac caatatccag aatggcatcc cacatcgatt tgaacgcctt
     2461 ccctgtttta tccgcccagg tttccagcgt ccccatgttc tctttcaggc ggcgggtctg
     2521 gtcatcaaag cctttcgtgg cagcctcgtt cgccgcctgc aatgccccgg cctcgtctcc
     2581 ggaacgctgc agctgcgcaa catacgcaat ctgctctgcc gtcacgttac ggaactggcg
     2641 cgccatcgcc atcagtcccg acgtcgggtc ggtggtcagt tttccgaaag cctctgcaac
     2701 cttgtccact tccacaccgg aggcagacgc aaaacgcgcg acactctggt tgatggaatc
     2761 aaactgttca ccaccacgca caccggcatt caccagggct gccagtgact cactcgcctg
     2821 gttaaacgtc agccctgctg cctgtccggc tctggagagc gtaagcatgc gctctgccgt
     2881 cagtcctgac tgattaccgg aaagaaccag ggttttatta aacgctgaaa gcgtggaatc
     2941 cccctggtac caggcgtacg ccagcgcacc ggtcgccacc gccagcgagg tgacaccggc
     3001 cattggcagg ctgatcgcac cagcaagccc ccggaacatg gggatcatcc cgccgaagga
     3061 gtccttcacc tgaccgccct gctgcagcat gataaggaag ggattctgac caccggcaag
     3121 ctgcgtggcc acgtcggtga actgcatggg tagcatacgc atggcggctt tatactgccc
     3181 gactgatatc ccggcttttt gtgcagccag cgcctggcgg ctcagcccct gctcaacagc
     3241 actggcggtt tttctggcgt cagtctccag tcctgaaaaa tgacgcctta cgcgggtcat
     3301 ctgctcatcg aaacgaaccg catccagact caggtcaata acaagatcac caaccggctg
     3361 ggacatatct cacacctccg gaaatccccg ctgaagccat cattaatgcg acatcatcct
     3421 cgctgacatc caccacatcc gcagaaggtg aaatatcgcc ccctccctcc tcgccgaacc
     3481 ggacgcctcc ggcaagtcct gccgctttct gcatcagcat gtcttcctca tccggcctct
     3541 ccgtctgcgc ttcctcacgc cgggggacga gcagactgaa atccgaggga tgcatatccg
     3601 gatcgcaaaa aaacaggctg agtacggcgt acgtcagccc ggaaaaatgc atatccagct
     3661 gggtatcgtg aaaataatgc gtgcggtaaa aacgtcgcca gtcggcatat tcggtggatg
     3721 tcatcccggc aagcatggcg cgccagtcgg gtctccccat ctcacgcgcc agtctgaggg
     3781 caaaattcag ctcgccgtcg aagactttcc cacagaaaaa tcatcatcag tcagcacgtt
     3841 atttttcgcc acttcggtga tatcagtatc cggacgaaca gcttcgatca tcccggacag
     3901 gcacaacaca acatcttccg cccgggcaat ggcatcggca ggccaggtgg tgagcacttc
     3961 ctgctctatc ttcatcacgg cctcactcat tgacggtgac tgcgttttct gtggatggtt
     4021 atgccacagg gacatcgcca ccagaaacgc gccggttctg acaagatctt ccacactcac
     4081 ctgcaggttg ccgctggctt cagcctcttc tgcccgccgt ttcaggaggg caagatgctc
     4141 aatacgctgc agcgcagaca gctcagaaag catgacggat acaccgttat attcaaattg
     4201 ttctgttttc aggaacatgc ttgatctcct cccctcagcc cgcagcgcca tccgtgacgg
     4261 tgatctccgc caccgccgca aactcaccgt tgccggtgac aacagggatc tgcgctttac
     4321 ctgccgcaac acctttcacg gtgatcgtgt tccctttcac ggtaatggtg gcgaaattct
     4381 gatttgccga cgttgcgcgg aatgttttat ccgttgcgcc ttccggctga acggccacag
     4441 tcagggtggt gctctgacct tttgccacat tccccgttag tggcgtcacg gtaataccgg
     4501 tgaccggtgt gatttctccc cgatcctccg ccagcgaagg acgaccgata ttggtgattt
     4561 ttaccgtacg ggtaatcact tctttggcgg tcaccgcttt accaatggcg ctcacccagc
     4621 cacggaacac atccaccgta ccgttcggga agcggatttt gtaggcccgg gtctcactgc
     4681 tgtcaaacca ggcaatcaaa tcacgctgcc ctttctcgcc gggcttccag gccagcgtaa
     4741 aactggtgtc accggcggat ttctgcccct gcccggtgga tacccagtcg gcatcctcat
     4801 catccagata gttatcgtcg tagggttccg ccgtcatctc gccgggggtc agatccttta
     4861 tttttgccag acgcgtccac tcatcatctg acagcgggtt tgcataagca tcgcccttac
     4921 cggtgtaaac ccagag
//
LOCUS       DAZKFU020000300         4862 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZKFU020000300 DAZKFU020000000
VERSION     DAZKFU020000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.302"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..278
                     /gene="gatB"
                     /locus_tag="ACHOQQ_RS21800"
                     /old_locus_tag="pgaptmp_004360"
     CDS             <1..278
                     /gene="gatB"
                     /locus_tag="ACHOQQ_RS21800"
                     /old_locus_tag="pgaptmp_004360"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HGU4472529.1"
                     /translation="KIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEIETY
                     MDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            282..1656
                     /locus_tag="ACHOQQ_RS21805"
                     /old_locus_tag="pgaptmp_004361"
                     /pseudo
     CDS             282..1656
                     /locus_tag="ACHOQQ_RS21805"
                     /old_locus_tag="pgaptmp_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            1704..2744
                     /gene="gatD"
                     /locus_tag="ACHOQQ_RS21810"
                     /old_locus_tag="pgaptmp_004362"
     CDS             1704..2744
                     /gene="gatD"
                     /locus_tag="ACHOQQ_RS21810"
                     /old_locus_tag="pgaptmp_004362"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4472530.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIVHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            2850..3623
                     /locus_tag="ACHOQQ_RS21815"
                     /old_locus_tag="pgaptmp_004363"
     CDS             2850..3623
                     /locus_tag="ACHOQQ_RS21815"
                     /old_locus_tag="pgaptmp_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4472531.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(3705..4604)
                     /gene="yegS"
                     /locus_tag="ACHOQQ_RS21820"
                     /old_locus_tag="pgaptmp_004364"
     CDS             complement(3705..4604)
                     /gene="yegS"
                     /locus_tag="ACHOQQ_RS21820"
                     /old_locus_tag="pgaptmp_004364"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGU4472532.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIVLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDNIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1190 a         1077 c         1277 g         1318 t
ORIGIN      
        1 gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc tacgatggcg gcggaagaaa
       61 ttaaagagtt gtgtcagagt cataatattc ctgttgaatt aatccagtgt cgggttaatg
      121 aaatagaaac ctatatggat ggcgtgcatt tgatatgcac cactgccaaa gtggatcgta
      181 gttttggcga tattccatta gttcacggca tgccttttgt ttctggtgtc ggtatcgaag
      241 cattacaaaa taaaattctg actatcttac aggggtgacc tatgttttca gaagtcatgc
      301 gttatattct cgatctcggc cctacggtga tgttgccgat tgtcatcatt attttttcta
      361 aaattttagg catgaaggca ggcgattgct ttaaagcggg tctgcatatc gggattggct
      421 ttgttggcat tggccttgtg attggcttaa tgctggattc cattggcccg gcggcgaaag
      481 cgatggcgga aaatttcgac ctgaatctgc atgtggtcga tgtcggctgg ccgggctctt
      541 caccaatgac ctgggcgtcg caaattgcgc tggtggcgat tccgattgcg attctggtta
      601 acgtggcgat gttactgacc cgtatgacgc gggtggtaaa tgttgatatc tggaatatct
      661 ggcatatgac cttcaccggc gcgttgctgc atctggcaac cggttcatgg atgataggga
      721 tggcgggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg tttgcccgcg
      781 atacccgaaa tttctttgag ctggaaggca ttgcaatccc gcacggtacg tcggcgtata
      841 tggggccaat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc gttaaccgta
      901 ttaaatttag cgccgacgat attcagcgca aatttggccc gtttggcgag cctgtcaccg
      961 tgggttttgt gatggggctg attatcggca tcctcgcggg ttacgatgtc aaaggtgtat
     1021 tgcagctggc ggtaaaaaca gcggcggtga tgctgctgat gccacgggtg attaaaccca
     1081 tcatggatgg tttaacccat catggatggt ttaacaccca tcgctaagca ggcccgtagt
     1141 cgtttacagg cgaagttcgg cggtcaggag ttcctgattg gccttgatcc ggcgttgctg
     1201 ctggggcaca cggcggtggt atcggcaagc ctgattttta ttccactcac cattttaatt
     1261 gctgtttgtg tgccgggtaa tcaggtgctg ccgtttggcg atcttgccac tatcggcttc
     1321 tttgtggcga tggcggtcgc ggtgcatcgt ggaaatctgt tccgcacctt aatctcgggt
     1381 gtcatcatta tgagcatcac cctgtggatc gcgacgcaaa ctattggttt gcacacccaa
     1441 ctggcggcta atgctggggc gttaaaagcc gggggtatgg tggcttcaat ggatcagggc
     1501 ggttctccca ttacctggtt actgattcag gttttctccc cgcaaaatat tcccggtttc
     1561 attattatcg gcgcaattta tcttaccggt attttcatga cctggcgtag agcgcgtggc
     1621 tttattaaac aagagaaagt cgttcttgca gaataatttt tacctgaggg ggaggttatc
     1681 ccccttaaca atcaggagtt tttatgaaat cagtggtgaa tgatactgat ggtatcgtgc
     1741 gcgttgcaga aagcgtcatt cctgaaatta aacatcagga tgaggtgcgg gtaaaaattg
     1801 ccagctcggg cttatgtggt tccgatttac ccaggatatt taaaaatggt gcacattatt
     1861 atccaataac gttaggccat gaatttagcg gctatattga tgcggtagga tccggtgttg
     1921 atgatttaca ccctggcgat gcggttgcct gtgtgccgtt attaccctgt tttacttgtc
     1981 cagagtgtct gaaagggttt tattcccagt gcgcaaaata tgattttatt ggctcacggc
     2041 gtgatggtgg atttgccgaa tatattgtcg ttaagcggaa aaatgtcttt gctctaccca
     2101 cggatatgcc tattgaggat ggggctttta ttgaaccgat taccgttggc ctgcatgctt
     2161 ttcatttagc gcaaggttgt gagaataaaa acgttattat tattggtgcc ggaaccattg
     2221 gcctgctggc tattcagtgc gctgtcgcgc tgggagcaaa gagtgtgacg gcgatcgaca
     2281 ttagctcaga aaaactagca ctggcaaaat ctttcggtgc gatgcaaaca tttaacagta
     2341 gcgaaatgag cgcgccgcaa atgcagggcg ttttacgcga gctgcgcttt aatcagctta
     2401 tcctcgagac ggctggcgta ccgcaaactg tcgaactggc ggtagagatt gccggtcctc
     2461 atgcccaact ggcgctggtg gggacgttgc atcaggatct gcatttaaca tcggcaacgt
     2521 ttggcaaaat attgcgtaaa gagctgacgg ttatcggcag ttggatgaac tactccagcc
     2581 cttggccggg gcaggagtgg gaaacggcga gccggttgct gacagaacgt aagttaagcc
     2641 tagagccatt aatcgttcac cgtggaagct ttgaaagctt cgcccaggcg gtgcgtgaca
     2701 tcgctcgtaa tgctatgccg ggcaaagtgt tgctcattcc ctgaaaccgc gggccagcgt
     2761 gatgctggcc cggtattgtg caaaacagat cattcaccaa tggtccccct tcgtttacac
     2821 tagccacaat tgaatatggg taaatgacga tgaattcatt cgagcgaagg aataagatca
     2881 tccaattagt gaatgaacag ggaaccgtgc ttgttcagga tctggcggga gtatttgctg
     2941 cctcggaagc gacaatccgt gccgatttgc gctttctcga acaaaaaggc gtggttacgc
     3001 gctttcatgg cggtgcggcg aaaataatgt ccggtaatag tgaaaccgag acccaggaag
     3061 tcgggtttaa agagcgattt cagctcgcca gcgcgccaaa aaacagaata gcgcaggcgg
     3121 cagtcaaaat gatccacgaa gggatgaccg ttattctcga cagcggaagt acgacaatgc
     3181 ttatcgccga aggattaatg accgccaaaa atatcacggt gattaccaac agtctcccgg
     3241 cagcgtttgc cctttccgaa aataaagaca ttactctggt cgtctgcggt ggcacggtcc
     3301 gccataaaac gcgctcgatg catggttcca ttgccgagcg ttcattgcag gatattaatg
     3361 ccgatttaat gtttgtcggt gctgatggta ttgatgcggt taatggtatt acgaccttta
     3421 atgaaggtta ttcgattagc ggtgcgatgg tcaccgctgc taataaagtg attgccgttc
     3481 tcgattcatc gaaatttaat cgtcgcggtt ttaatcaggt actgccaatc gaaaaaattg
     3541 acattattat taccgatgat gctgtgtcag aggtggataa actggcattg cagaagacac
     3601 gggtaaaatt aattacggtg tagtattgtt ctgtatgccg gataaggtgt ttacaccgca
     3661 tccggcatta cggattctct ctggttcaag agagaatagt ttgattaacg caataatggg
     3721 caatctggtg gtaatcgaca acgcaacgcc gccggaagta tttcaatatg aaagttttgc
     3781 ccactcaacg gttcgccatc aagattaaaa gtgatttcgt gtggggcttg tatatcaaac
     3841 cacgacgaag cgccttcgat aatattcggg ttatcttcgt cagattttaa ggttgatacg
     3901 agagctggaa gaatttcatc gccggtaaaa atgcgcagtt gcagcaagcc atcgttaatt
     3961 aacgcgttcg gacacaattg ctgaccgcca ccggcctgac gcccgttacc aataccaatg
     4021 accagggcgt caccttgcca gtgaaagttt tcaccgcgga tttcacaacg gtccggttgc
     4081 agagtatcca tgcgcattaa gccatgaatg atgtaagaga cgccacccag cgcggctttt
     4141 aatttttccg gcgtttctgt ggtaatacgc gtcccaaacc cgcctgtcgc catattaata
     4201 aaacaggttt gtttgttgac ctgcgccata tctatcgcaa tggcgttacc ggcaattgcc
     4261 agtttcagcg ccttatccag tgcctcagga atccctacac tggtggcaaa atcattggcg
     4321 gttcctaatg gcaaaattcc cagcgcgggt atgttatccc cctcacactg aatcaacgcc
     4381 gtagaaactt cattaatggt gccatcgcca ccaccggcaa tcaccgttgc gacgcccaac
     4441 ttccgggcct cctctacata tcgtgcggca tcgcctttct cccaggtgac ccgcacatgg
     4501 atcgtcattc cttcctcacg caacagcaca attgcttcgc gcaatggtaa attgtcagta
     4561 cttttgccat taagaatcag taagctggcg ggaaattctg ccatgatcgt atgtgccttt
     4621 atgattggtc tgtagatagt gtagagcaga aaagaaaagg tggagtcaga acaggatgaa
     4681 agtcggagga taaaaagtca gttcaagtat ggaggattca gtggttacca ccaggagctt
     4741 gcaattggga cagcgaatac taacggcagc caccgataaa tgtcaaaaaa gagcatatac
     4801 ctaatattca actaaacagt ggcatcttca atataatata ttaaagcccc catggagtta
     4861 cc
//
LOCUS       DAZKFU020000301         4847 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZKFU020000301 DAZKFU020000000
VERSION     DAZKFU020000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.303"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            16..732
                     /gene="nanC"
                     /locus_tag="ACHOQQ_RS21825"
                     /old_locus_tag="pgaptmp_004365"
     CDS             16..732
                     /gene="nanC"
                     /locus_tag="ACHOQQ_RS21825"
                     /old_locus_tag="pgaptmp_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGU4472533.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            752..1858
                     /gene="nanM"
                     /locus_tag="ACHOQQ_RS21830"
                     /old_locus_tag="pgaptmp_004366"
     CDS             752..1858
                     /gene="nanM"
                     /locus_tag="ACHOQQ_RS21830"
                     /old_locus_tag="pgaptmp_004366"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU4472534.1"
                     /translation="MNKTITALAIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALASFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRDNYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1923..2903
                     /locus_tag="ACHOQQ_RS21835"
                     /old_locus_tag="pgaptmp_004367"
     CDS             1923..2903
                     /locus_tag="ACHOQQ_RS21835"
                     /old_locus_tag="pgaptmp_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4472535.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDGEDALHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALTKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(2911..3561)
                     /locus_tag="ACHOQQ_RS21840"
                     /old_locus_tag="pgaptmp_004368"
     CDS             complement(2911..3561)
                     /locus_tag="ACHOQQ_RS21840"
                     /old_locus_tag="pgaptmp_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4472536.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIDQGFHIHQDKAAAAPIADVLFPD
                     ELPSQGTYVEGAAIKVTVNSYERDKKAREKAVEYHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYLLDPVNDLLPVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            3698..4570
                     /locus_tag="ACHOQQ_RS21845"
                     /old_locus_tag="pgaptmp_004369"
     CDS             3698..4570
                     /locus_tag="ACHOQQ_RS21845"
                     /old_locus_tag="pgaptmp_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HGU4472537.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIVLPQVRKNYPY
                     EGFIEWHRKEVFRG"
     gene            4615..4794
                     /locus_tag="ACHOQQ_RS21850"
                     /old_locus_tag="pgaptmp_004370"
                     /pseudo
     CDS             4615..4794
                     /locus_tag="ACHOQQ_RS21850"
                     /old_locus_tag="pgaptmp_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         1381 a         1070 c         1159 g         1237 t
ORIGIN      
        1 aaatgagtga aatttatgaa aaaggctaaa atactttctg gcgtattatt actgtgcttt
       61 tcgtcaccat taatttctca ggctgcgaca cttgacgtac gtggtggata tcgtagtgga
      121 agccacgcct atgagactcg actcaaagtc agtgagggat ggcaaaatgg atggtgggca
      181 agcatggaaa gtaatacctg gaataccatt catgataata aaaaggaaaa tgccgcactc
      241 aatgatgttc aggttgaagt taattacgcg attaaacttg atgatcaatg gacggtgcgc
      301 ccgggaatgt taacgcattt tagcagcaac ggcacacgct acggacccta cgtaaaactg
      361 tcctgggacg cgacaaaaga tcttaatttt ggcattcgct atcgttacga ctggaaagct
      421 taccgacaac aagacttatc cggtgatatg tctcgtgata acgttcatcg ttgggatgga
      481 tatgtcactt accatattaa tagtgatttc accttcgcat ggcaaacgac gctatacagc
      541 aaacagaacg attatcgcta tgcaaaccat aagaaatggg cgacggaaaa tgcatttgtt
      601 ctacaatacc atatgacgcc cgatattacg ccatacatag aatatgacta cctggaccgt
      661 cagggtgttt acaacggcag agataattta tcggaaaaca gttatcgcat tggtgtgtca
      721 tttaaactgt agtagacagg agacagtcac aatgaataaa acaataacgg cgcttgctat
      781 cataatggct tcatttgccg caaacgcgtc tgtattaccg gaaactcctg tgccatttaa
      841 aagtggtacc ggagcaattg ataacgacac tgtctacatt ggtttaggta gcgcaggtac
      901 ggcatggtac aagctggaga cacaggccaa agataaaaaa tggacagcgt tagcttcatt
      961 ccctggcgga ccaagagatc aagcaacctc ggcatttatt gatggcaatc tgtatgtgtt
     1021 tggcggcatt ggcaaaaaca gcgagggctt gactcaggta tttaatgacg tacacaaata
     1081 caaccccaaa accaatagct gggttaaatt gatgtcgcac gcaccgatgg gcatggcggg
     1141 tcatgtgact tttgtacaca acggcaaggc ttatgttact ggcggtgtta accagaatat
     1201 cttcaatggc tattttgaag atctcaacga ggctggaaaa gattcaaccg ctatagataa
     1261 aatcaacgct cactattttg acaaaaaagc agaagattat ttcttcaata agtttctgtt
     1321 gtcttttgat ccctcaaccc agcaatggag ttacgctggc gaatcgccct ggtacggaac
     1381 ggctggtgcg gcggttgtga ataaaggtga taaaacctgg cttattaatg gtgaagccaa
     1441 accaggattg cgaacggatg ccgtatttga acttgatttc accggtaata atttaaaatg
     1501 gaataggctt gctcccgtct catcaccaga tggcgtcgct ggcggttttg cggggataag
     1561 caatgattct cttatatttg ccggaggggc cggattcaaa ggttcacgag ataattacca
     1621 gaacggtaag aactatgcgc atgaagggct gaaaaaatca tatagcgctg atattcatct
     1681 ttggcataac gggaaatggg ataaatcggg tgaattatcg caaggtcggg cctacggagt
     1741 atcattgccc tggaataata gtctattgat tattggcggt gaaactgcag gcggcaaagc
     1801 ggtgacggat tcagttttga tctctgtgaa ggataacaaa gttactgtgc aaaactaacg
     1861 cttcagggcc ccagtaaagg ggccctgcta tcaatttgtc atttcaatta cgcgaaaatt
     1921 atatgaacgc aataatatcg ccggattatt actatgttct taccgttgct ggtcagtcta
     1981 atgccatggc gtatggcgaa ggactgccat taccggacgg ggaagatgcg cttcatccca
     2041 gaattaaaca attagcgaga tttgcgcata cgcatcccgg aggcccgtca tgtcacttta
     2101 acgacattat tccactgact cactgcccac acgatgttca ggatatgcag ggttatcacc
     2161 atcctctggc aacgaatcat caaacacagt acggcaccgt tggccaggca ctgcatattg
     2221 cacggaaatt actgcccttc attcctgata atgcaggggt tctcatcgtt ccatgttgtc
     2281 gtggcggctc ggcttttatc gcaggcagcg aagggacata ttcagaacgg cacggagcca
     2341 gccatgatgc ttgtcgttgg ggaacggata ctccgctata ccaggattta gtcagcagaa
     2401 cgcgagccgc actgacaaaa aatccgcaga acaaattcct cggcgtatgc tggatgcaag
     2461 gcgaatttga cttaatgacc agtgactacg cgtcacaccc tcaacacttt aatcatatgg
     2521 ttgaagcctt tcgtagggat ctaaaacaat accattctca gcttaataat attactgacg
     2581 cgccgtggtt ttgcggcgat accacctggt actggaaaga aaatttccct catgcgtatg
     2641 aagctattta tggcaattat caaaataatg ttttagccaa tattattttc gtcgacttcc
     2701 agcaacaagg tgaaagagga ctgacgaacg cgcctgatga agatccggac gatttaagca
     2761 cgggatatta cggttcagcg taccgctcac cggagaactg gacgacggca ctgcgaagca
     2821 gtcatttcag cgcggcagcc cgtcggggga ttatttctga caggtttgta gaagcaattt
     2881 tgcagttttg gcgcgaaagg tgagcgttca ttaatttgat ttattcgcat ccatcaacgc
     2941 ttttaactcc tccggcgtaa acggtggttt gcgtcggtgt aacatcgcat ggcaattagg
     3001 gcacacagga agtaaatcat taactggatc cagcaggtaa tcctctttaa tggctgaaag
     3061 cggcaccagg tgatgcatat ggataaagcc ctcagcgata tcgccataga tcttcaccag
     3121 atcaacgccg catacgttgc actggcatcc gtgatattcg actgctttct cgcgagcttt
     3181 tttatcgcgt tcatagctgt ttaccgtcac ctttatcgcc gcaccttcaa cgtaggtccc
     3241 ctgcgacggc aactcatccg ggaacagaac atcggctatg ggagccgcag cggctttatc
     3301 ctgatgaata tgaaatccct gatctataag tagcttgacg catttagatt ttattccgcc
     3361 ggtgaaatcc gcaggggtaa attgcgtccc cgtcatcaat gtcgctgcga tgccagcaat
     3421 cgccttcgag gggtaacgtc ggccatgata ttcaatctca tacaaccgtg caggctggaa
     3481 ctgatgagtt tctcccgcat cccataaatg aatagcttcg ataatatacc gccgcggaag
     3541 atgatcgggg agtttattca tagagaatgt ccttatacgt tctcagaaca agcagcaaaa
     3601 aaacaaatga gatcaaagta atatatcttc acttgcaggc ttgaacagca gaaaatttgc
     3661 gagcatgatc gcaattattt cactgccagg gatatcaatg gcttccagta aatcgctaca
     3721 gcaggcaatt gccaacataa aaatctggca caagggtgaa cagcgcgcgc cgcataaacc
     3781 attgttactg ctatacgtat tagcgggata cctgaatgga catccacgcc ttttcgatta
     3841 tggatcggaa atctacgaac ctctgcacag tttactggaa cgttttgggc cacagcgttc
     3901 acagtaccga cctgacatgc cgttctggcg attacaaggc gacggattct ggcaattaca
     3961 caatgccgag ctttgctcaa cagcagggag cagccgacaa ccgccagtga aggaactgaa
     4021 cgaataccac gttgcaggcg gtttcgatga acagcactac gccctggtaa ccggcaataa
     4081 aaaactcatc aatactctgg cgcaacagat actcgaagcg cacttcaccg agagtattca
     4141 ggaagaaatc gctgacgagc tgggttttga tctccagcaa atccgtaaac agcgcgaccc
     4201 gttatttcgc aaaaatgtat tacgcgctta taactaccag tgtgcgatat gtggctttaa
     4261 tatgcgccac gacgacacca ccgtcgcgct ggaagcggcg catataaaat ggaaacagca
     4321 cggcggtcct tgtgaaattc ctaatggcct ggcgctttgc gcaatacacc ataaagcctt
     4381 tgataaagga tcgattggcc tggacgaaaa tatgcgggta ctggtatcag atgcggtaaa
     4441 cggcggaggg atcgttgaga gattattctg ggattttgac gggaaaacta ttgtgttgcc
     4501 ccaggtgcgt aaaaattacc cttatgaggg gtttatagag tggcatcgga aagaggtttt
     4561 tagggggtga ggttgataac tatcgcagta tgttcaatat tgctgacata atcagtgcag
     4621 gatcttgccg ctagtttaca attcgccttc gatagcggcg caaaacgagt tctgttgccg
     4681 atatcctcgg ctatggatat tccaacagtt ccggcagagt tgtttaccaa gtttcaggta
     4741 agtttttact cagaaccggt tgatgcggtt tataaggcgc tggatgtgaa ttaacacatt
     4801 cctcagctta tttataaaag gattattccc atttgatata atatcag
//
LOCUS       DAZKFU020000302         4817 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZKFU020000302 DAZKFU020000000
VERSION     DAZKFU020000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.304"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            278..436
                     /locus_tag="ACHOQQ_RS21855"
                     /old_locus_tag="pgaptmp_004371"
                     /pseudo
     CDS             278..436
                     /locus_tag="ACHOQQ_RS21855"
                     /old_locus_tag="pgaptmp_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            660..779
                     /locus_tag="ACHOQQ_RS21860"
                     /old_locus_tag="pgaptmp_004372"
     CDS             660..779
                     /locus_tag="ACHOQQ_RS21860"
                     /old_locus_tag="pgaptmp_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU4472538.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            844..>4817
                     /gene="ypjA"
                     /locus_tag="ACHOQQ_RS21865"
                     /old_locus_tag="pgaptmp_004373"
     CDS             844..>4817
                     /gene="ypjA"
                     /locus_tag="ACHOQQ_RS21865"
                     /old_locus_tag="pgaptmp_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HGU4472539.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPGMAAFTSDVIGVVNDETV
                     DGTQRVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDVGSHNNFVGQANNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTVKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSTSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDGTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGSVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTKINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSH
                     IGFDR"
BASE COUNT         1396 a         1062 c         1262 g         1097 t
ORIGIN      
        1 taaatgggat tattcttcac actacatcca ttttagtgtc actaccaaag aaaaaggggc
       61 tacgttttca tgtaacccct tgatttattt ggtgggctag cagtgactga attggtgctg
      121 taattggttg aaatatttca tctaatgtct ggttcaactt ccttagctgc ctgaaaattt
      181 tttgagctta cccagcaata acagcgttac ggtgcaccac gcctgactga agaactgcgt
      241 gctcagggtt accacttcaa cgtaaaaccg tggcaggagg tccggactga aaaaactgct
      301 caggacatca tcggcaggga acgcactgct agtctgtaaa ctgaaccgac tggggcgcag
      361 tatgtggcat cttgtcgtgc tgttggagga gctgtgcgaa cgtggtatta actttcgtgc
      421 tctggcccaa tctatatttg cccaacaatg gggggacgaa tgctgtaaaa gtaaaacaat
      481 ctgcgatctc aaagttattg tgtgattttt atgtgagcag aagatattca tcagaaacga
      541 ttatgtaaat cattttattt tgccaacggc ctcattgtcg aaagaaaaga atacgcccgt
      601 gtttcctgaa tagattgact tttttatcca accacacttc agcgcactgc gtttaaaaaa
      661 tgcctcattc ttatgcggaa tatcatcatt tcatcatgat gtctttgatg agcggtgaac
      721 acaatacact tgcgctgtct cttcaggatg aatcccctgg tctggtgcct gtgggctgat
      781 gttgcagcaa agctaaggtc gcttaaacgc tactcagtat tcacttttca gaggatgaaa
      841 tttatgaaca ggaccagtcc ccattattgt cgccgctcag tactttcctt attgatatct
      901 gccttgatat atgccccgcc cgggatggcg gccttcactt ctgatgttat tggtgtggta
      961 aacgatgaga ctgtagatgg cacccaaaga gtagatgaac gaggtacaac aaataacact
     1021 catattatca accatggcca gcagaatgtt catggcgggg tatctaatgg aagtcttatt
     1081 gaatctggtg gatatcaaga tgtaggaagt cataacaatt ttgtggggca ggctaataat
     1141 acaaccatta acgggggcag acagtcaatt catgacgggg gtatttccac aggtacgata
     1201 atcgagagtg gcaatcagga cgtttataaa gggggtatca gcaatggaac gacagttaag
     1261 ggcggtgctt cacgcgtaga gggagggagt gcgaatggaa cactcattga tggtggtagc
     1321 cagatagtaa aagttcaagg gcatgctgat ggtacaacga taaataagtc tggctctcag
     1381 gacgtagtac aaggaagtct ggcaacgaac acaaccataa atggtggtcg acagtatgtt
     1441 gaacagagca cagtagaaac aaccaccatc aaaaatggcg gtgagcaaag agtatatgag
     1501 agccgtgcgc tggacacgac gattgaaggc ggaactcagt ctctgaatag taagtcaacg
     1561 gcaaaaaata cgcagatcta ttctggtggc acgcaaattg ttgataacac cagcacctcg
     1621 gatgttattg aagtttattc cggtggcgtg cttgatgtta gtggtggtac ggcaacaaat
     1681 gttacccagc acgatggtgc aattttaaaa actaacacta acggtacgac ggtgagcggt
     1741 acgaatagtg aaggtgcatt ctccatccac aatcacgtgg cagacaatgt gttgctggaa
     1801 aacggtggtc atttagacat aaacgcatat ggttcggcaa acaagacgat tattaaagat
     1861 aaaggaacaa tgtcagtttt aaccaatgct aaagctgatg cgacccgaat agataatggc
     1921 ggggttatgg atgttgcagg aaacgcgaca aataccataa ttaatggtgg cacacagaat
     1981 attaataatt atggcatagc cacaggcacc aatatcaaca gcggaacgca aaatatcaaa
     2041 agcggcggga aagctgacac aacaattata tcctccggga gccggcaggt tgttgagaaa
     2101 gatggtacgg caattggcag caatattagc gccggaggct cgctgattgt ctataccggc
     2161 ggtattgcac atggggttaa ccaggagacg ggcagtgctt tagttgccaa cacgggtgca
     2221 gggactgata tcgaaggata caacaagctc tctcacttca ctattaccgg aggggaggct
     2281 aattatgttg tgctggaaaa taccggcgaa ctgacggtag tggctaaaac ctcggcgaaa
     2341 aatactacca ttgatgctgg cggtaagctg attgtccaga aggaggctaa aacagatggc
     2401 accagactta ataatggcgg cgttctggag gttcaggacg gtggtgaggc taagcatgtt
     2461 gagcaacaat ccggcggcgc attaattgct tccacgacct ccggaacact tatcgaagga
     2521 accaacagtt atggtgatgc tttctacatc aggaattcag aagctaaaaa tgtagtgctg
     2581 gaaaacgctg gctcattaac agtcgtcact ggttcccggg cagttgacac gattattaat
     2641 gccaacggca aaatggatgt ttatggaaaa gatgttggct ctgtactcaa tagtgctggc
     2701 acccaaacaa tatatgccag tgccacttct gataaagcaa atatcaaagg tggcaagcaa
     2761 acggtatatg gtttagccac tgaagcaaat atcgaaagtg gtgaacaaat tgttgatggt
     2821 gggtcaacag agaaaacaca catcaatggt ggcacgcaaa ccgttcagaa ttatggtaag
     2881 gcgatcaata ccgatatcgt ctctggccta caacaaatta tggcaaacgg gacagcggaa
     2941 ggttccatta ttaatggcgg ttcacaggta gttaatgagg gcggtctggc tgaaaactcg
     3001 gtgcttaatg acggcggcac actcgatgtg cgggagaaag gcagcgcaac ggggatacag
     3061 cagagtagcc agggcgcgtt ggttgcaacc accagggcga cgcgggtcac aggaacacgc
     3121 gcggatggcg tcgcgttcag catcgagcag ggtgcggcga acaatatcct gctggcaaat
     3181 ggcggcgtgt taaccgtgga gtcagacacc tcttctgaca aaacacaggt caatacgggc
     3241 ggacgggaga tcgtcaaaac aaaagccact gcgacaggca cgacgctcac cggcggtgaa
     3301 caaattgtcg agggtgtggc gaatgagaca acaattaacg acggcggaat acaaacagtt
     3361 tcagctaacg gagaggcaat aaaaacaaag atcaatgaag gcggtacgct gacagtcaac
     3421 gataatggca aagcgacaga tatcgtccag aacagcggtg ccgctctcca gacgagcacg
     3481 gctaacggta ttgaaatcag cggtactcac cagtacggca ctttttccat ttccggcaat
     3541 ttagcgacca atatgttgct ggaaaatggc ggtaatttat tggtattagc aggtaccgaa
     3601 gctcgcgact ccacggttgg caagggtggg gcaatgcaaa acctgggtca ggactccgcc
     3661 acaaaggtta actctggcgg gcaatatacc cttgggcggt caaaagatga gtttcaggct
     3721 ctggcccggg cagaagatct ccaggtcgct ggcgggacgg caatcgtcta cgcaggtacg
     3781 ctggcggatg catcggtcag tggcgcgaca ggaagcctgt cgttaatgac gccacgggat
     3841 aatgttacgc cagttaaact cgaaggggcg gtccggatta ccgatagcgc gacattaact
     3901 ctgggaaatg gcgtcgatac aacgcttgcc gacctgacgg ctgccagccg gggcagtgtc
     3961 tggcttaaca gcaataattc ctgtgcaggt accagcaact gcgaatatag agtaaacagt
     4021 ttgctactca acgacggtga tgtttattta tcagcacaaa cagcagcgcc tgccacaact
     4081 aacggtatct acaatacgct gacaaccaat gaactttccg gtagcggtaa tttctacctg
     4141 cataccaacg ttgcaggctc ccggggcgat caactggtcg tcaacaacaa cgccactggt
     4201 aattttaaaa tctttgttca ggataccggc gtcagcccac agtctgacga cgcgatgacg
     4261 ctggtgaaaa cagggggagg ggatgcttcg ttttcgctgg gcaatactgg cggtttcgtt
     4321 gatcttggga cctatgagta tgtcctgaaa agcgatggca acagcaactg gaacctgacc
     4381 aatgatgtca aacccaaccc ggatcccaac ccaaatccca acccaaatcc gaagccggat
     4441 ccaaaaccag acccaaaacc ggatccgaaa ccagacccga ctcccgatcc aacgccgaca
     4501 cccgttccgg agaaacgcat cacgccttct acggcagccg tactcaatat ggctgcaaca
     4561 ttaccgttgg tatttgatgc tgagctaaac agtattcgcg agcggttgaa cataatgaaa
     4621 gcgagtccac acaacaataa tgtctggggg gcgacgtata acacccgtaa taatgtcacc
     4681 accgatgcgg gtgccgggtt tgagcagacg ctgaccggaa tgacagtggg gatcgacagc
     4741 cgtaatgata ttcctgaagg aattgccacg ctgggcgctt ttatgggtta ttcccattca
     4801 catatcggtt ttgatcg
//
LOCUS       DAZKFU020000303         4805 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZKFU020000303 DAZKFU020000000
VERSION     DAZKFU020000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.305"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            158..724
                     /gene="slp"
                     /locus_tag="ACHOQQ_RS21870"
                     /old_locus_tag="pgaptmp_004374"
     CDS             158..724
                     /gene="slp"
                     /locus_tag="ACHOQQ_RS21870"
                     /old_locus_tag="pgaptmp_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGU4472540.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            880..1410
                     /locus_tag="ACHOQQ_RS21875"
                     /old_locus_tag="pgaptmp_004375"
     CDS             880..1410
                     /locus_tag="ACHOQQ_RS21875"
                     /old_locus_tag="pgaptmp_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGU4472541.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1471..2499)
                     /gene="chuS"
                     /locus_tag="ACHOQQ_RS21880"
                     /old_locus_tag="pgaptmp_004376"
     CDS             complement(1471..2499)
                     /gene="chuS"
                     /locus_tag="ACHOQQ_RS21880"
                     /old_locus_tag="pgaptmp_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HGU4472542.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAV"
     gene            complement(2548..4530)
                     /gene="chuA"
                     /locus_tag="ACHOQQ_RS21885"
                     /old_locus_tag="pgaptmp_004377"
                     /gene_synonym="shuA"
     CDS             complement(2548..4530)
                     /gene="chuA"
                     /locus_tag="ACHOQQ_RS21885"
                     /old_locus_tag="pgaptmp_004377"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HGU4472543.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     IDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1276 a         1169 c         1089 g         1271 t
ORIGIN      
        1 tcatgccacg ccccaaaata acggaattga actaaacaga agggatataa acatcagaca
       61 ggtttacgtt actatcaggc atatcgcctc agaatcagat gaaaactata aagaaatatc
      121 tattatggtt ttaatatttg ttgataagga tagtaacatg aacatgacaa aaggtgcact
      181 catactcagc ctttcatttt tgcttgccgc atgtagttca attccgcaaa atatcaaagg
      241 caataaccaa cctgatattc aaaaaagttt tgttgctgtt cataaccagc cggggttata
      301 tgttggtcaa caagcgcgct ttggtgggaa ggttatcaac gttatcaatg gcaaaacgga
      361 tacgttgtta gaaatcgctg tattaccgtt ggatagctat gcgaagcctg atattgaagc
      421 caactatcag ggccgactgc tcgccagaca aagcggcttc cttgatccag tgaactatcg
      481 taatcacttt gttaccatcc tcggcaccat tcagggtgaa caacctggct ttatcaataa
      541 agtcccgtat aacttcctgg aagtgaatat gcagggcatc caggtgtggc atttgagaga
      601 agtggttaat accacctata acctgtggga ttacggctat ggtgcattct ggccggaacc
      661 gggctggggt gcgccttact acaccaatgc ggtgagtcag gtaacacctg agctggtcaa
      721 ataacaccac cgaaagatgc agatgctttc tcagcatctg catcatgtat tacataaaat
      781 taatatacag taagctaact attattatta taagccctgt cctgttaatt acctttggca
      841 aactgattat aaagttaatg tccgcaccag gagtcggtta tgtttcttat aattaccagg
      901 gatacgatgt tcttcaccgc gatgaaaaac attctgagta aaggtaatgt cgttcatata
      961 cagaacgaag aagagatcga cgtaatgttg catcagaatg ccttcgtcat tattgataca
     1021 ttaatgaata atgtatttca ttctaatttt ctcactcaaa ttgaacgatt aaaacctgtc
     1081 catgtcatta ttttctcccc ctttaatatt aaacgctgcc tggggaaagt gccggtgacc
     1141 tttgttccac ggactatcac tatcattgat tttgtcgcac tcatcaatgg cagttactgc
     1201 tctgtgcctg aagcgaatgt gtcactttcg cgcaagcaac atcaggttct gagctgcatt
     1261 gcgaatcaaa tgacaacgga agatattctg gagaaactga aaatatcgct aaaaacgttc
     1321 tactgccata aacacaatat catgatgatc ctcaatctta agcggatcaa tgagctggta
     1381 cgccatcagc atattgatta tctggtgtga acgattgatc aatataaaga ggcccagcaa
     1441 taaatagcca gacctcccgt taattatacg ttatacagca acgccttccg gtatcagcga
     1501 agcaatttgc ttacgccatt gcgcttgctc ctgttcgcct tctgtacgtt gaccataaag
     1561 ttgcgctatc tgcgtaccat catgggcaaa caattccaga ctggttacgt agccatcgct
     1621 ggtcggttta cgggtaaccc aggcttcagc aatgctctct tctaatagat gaagagtaaa
     1681 cgtcgggttg aaaatattca gccaaccttt cattggcacc actttttcta ccacaccggt
     1741 gaaaatctgt acgcagccac ggttgccaac aaacaccatg atttcattac catcctgctg
     1801 tgcagattca agaatttgcg ccaacgcact gttggatact ttgcaggcca aatcgtctgc
     1861 caccagattg aacgcctgtt ggcgcgtcag gttgtggcgc ttgagcaacg taaaaaactg
     1921 atgaacgtcg gtcatcgccc gccactcttg ctcgaccaca gtggcatcgg ctcgcgtttg
     1981 aacaactggc gcatcaacgg cttttaactc aagcggcgta ttctcatcgg tgataaaccg
     2041 tgccagaagc tcactccatg ccgccatatc ggtattgtcg gtggcataaa cttttagtaa
     2101 tgcatcgccc tgatgatcaa agaactgaat actctggcgt tcgccacgag ccgtgttttc
     2161 tttgatgtgg aaaacactgg cccattgatt gagaaacaga cgtaaatcca gcgcgcgcgg
     2221 attgaggatc aatccggcat gtccgttcaa atgctggttt gtgaacgtac caacttgctc
     2281 atggactgca tattcattac gacaaataca tttggtttcg ccaacacttt cgagcgccgc
     2341 cagaatttca cggatatcgc cgtgcatccg ccacgcatcg tgcgtgacgc gtgcaaatgc
     2401 cagttctgct tcgctgatat tcattaaacc tgcgatgtca cgcgcgtact ttccgggatt
     2461 ttgttctttt aactcaagcc agcgtgtgta gtggttcatt gtctctcttc cttccagata
     2521 aatgccccga aatatcgggg cagatgatta ccattgataa ctcacgaaaa tttttccgtt
     2581 acgaccatcc tgtgggatgc cttgcggcga ccagtactct ttgtcgaaag cgttacccaa
     2641 caccaaagta gtggtcatac ctttgagcgc ctgttgtcct tgataactga cgtagaaatc
     2701 attcacgcca tagcctggtt gtttgctgta actgctgctg atatgtgttg agcgatcggc
     2761 aaacgtacca acccacccaa cagagaagcc actgtgagcg atcggaatat tcagagtgct
     2821 ggtaacggta tccgggttaa tgctggagat gtattcgccg gtatcggtgt ctttgccgcg
     2881 ggtacggtta taggccacat caaggctaaa cagatcagtg gtatatttcg tcatcacatc
     2941 ccagccccag attttggcgt tcgggacgtt atacgacata gtcgtcgccg ccgcgaaatc
     3001 gacggtcgtg gagatgtaat ccttcgcttt ggtatcaaag tagctggctt taaattccag
     3061 agcatcattg gacaacatca ggtcatcaaa acgcagccca aaaccgtact cctgagtttc
     3121 gttagtttcc ggacgtaagt tcgggtttgg cacccaatag ttggtataga agcgaccaat
     3181 cgagaagtgc ttagaatcgt tatacatttc gcccatcgtc ggggcgcgga atgcctgggc
     3241 atatgagcca aataacatca gccagttagt cggattgata gtcatccccg cacgagatga
     3301 ccatttgtcg gcatcaacat ctttgtaacc gtcactgcta ccgcgataac tgtcatagcg
     3361 ggttccgcca agcagggtaa tcggcagatc gcgtaaggtg atctcatcct gtagccagcc
     3421 ggagctaaaa tcgatttttg cttgcgggaa gcccgtcgtc gcgccgcccg gatgttgttc
     3481 ctgacgataa tactcaccgc catatgtcag taagtgagaa gcgaaactgt cggcaaagag
     3541 agtggaacgg ttctccagcc tggctccttt tgttatctgt tcacgatact cgccggaact
     3601 ccccgtgttt tgcgcattaa tacggacttc cgaccaataa atttttgcat ctgcatttaa
     3661 ccagtcgttg ccctgcgggg cgagtttata agaaagctgc gcatcgcgtt gaattgttga
     3721 acgatcaacc atcgggttgc tgctttcaga agcctcaacg gtctgcggat tttttggttc
     3781 acgcgcgtcg ttgttgtagt aacgcactaa accgctcaga gactgggctg aatcaatttg
     3841 ccaggtccct ttcgccagca tgttattaat ggactcgtca ttcggcgcgg tttcaccatt
     3901 gctctggcgt aaatcacccc gatcgcgact ggaccaggcc acaataccat ccagattttc
     3961 agttcgccca aacgcgctcg cgcctaatcc caggctatgg tcccccgtgc cgccagtacc
     4021 aaagacacga aaaccactgc tttgtccttc ctgcaataaa tcttttgcat cgaccgtatc
     4081 gtaggatatc actccaccca gcgcgccact gccatacagt aatgctgaag gtccgcgaac
     4141 aatctcaaca cgcttgatca gcgccggatc gagaaaagtg ccattcaggt gtccggtatc
     4201 cgttccctgg cgaacaccat cgataagaac cagcacgccg cgatgatcat agccacgcat
     4261 atttacatcc tgaccgttgg ttcgtccggt accatccaga gtaattccag gaacatgacg
     4321 cagcagatcg gtggctgaag tagccgtttg attttcagga gcggaagtgt cgatgacgct
     4381 gaccatcata ggcgcttcga aggaactacg ggcattcccc gttgccgtaa cggtcatggt
     4441 ttcagtagca aaagcaaacg ttggcaaggt ggcagaaaca gccaaggcca ataaactcaa
     4501 acgcaacgag gtaaattgcg gacgtgacat agcgattctc catgaggaaa ataaaaaatg
     4561 ctggctgcct gggttaatac cggggtggct ggcgaaaaat aaatttattt aaaattcaga
     4621 gcgataatat tcgccgggtg agtttacagg cgtttatatc ccatgaaatt gcctgataac
     4681 tcggcaatta cgcaaaagca cgccctcttt gtcaggcaaa acgatatgcc ccatcaggag
     4741 tgaagagagc tgtgcgttat cccttgcaaa tgctatggtt ttgttatcca tatggtcgtg
     4801 ttttg
//
LOCUS       DAZKFU020000304         4764 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZKFU020000304 DAZKFU020000000
VERSION     DAZKFU020000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.306"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..275)
                     /locus_tag="ACHOQQ_RS21890"
                     /old_locus_tag="pgaptmp_004378"
     CDS             complement(<1..275)
                     /locus_tag="ACHOQQ_RS21890"
                     /old_locus_tag="pgaptmp_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4472544.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMA"
     gene            complement(275..604)
                     /locus_tag="ACHOQQ_RS21895"
                     /old_locus_tag="pgaptmp_004379"
     CDS             complement(275..604)
                     /locus_tag="ACHOQQ_RS21895"
                     /old_locus_tag="pgaptmp_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4472545.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(601..3246)
                     /locus_tag="ACHOQQ_RS21900"
                     /old_locus_tag="pgaptmp_004380"
     CDS             complement(601..3246)
                     /locus_tag="ACHOQQ_RS21900"
                     /old_locus_tag="pgaptmp_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4472546.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEDSLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKDVPGVSALFGELAEENREFIEKTKRDELALRKSIAERDARIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEA
                     ELRTLEKHSGANEKISQQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLAHEKETLEY
                     KRQLAELGDKVEYQKRLNELAQQAARFEEQQSAKQAAISAKARGLTDRQAQRESEEQR
                     LRDVYGDNPQALAQVTGALKQTWADEDMLRGDWLAGLKSGWGEWTESATDSFSQVKSA
                     ATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAF
                     GGGASASTGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGN
                     LYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYD
                     MARKGAQDELRLQLRDGGMLSGSGR"
     gene            complement(3296..3598)
                     /locus_tag="ACHOQQ_RS21905"
                     /old_locus_tag="pgaptmp_004381"
                     /pseudo
     CDS             complement(3296..3598)
                     /locus_tag="ACHOQQ_RS21905"
                     /old_locus_tag="pgaptmp_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(3625..4047)
                     /locus_tag="ACHOQQ_RS21910"
                     /old_locus_tag="pgaptmp_004382"
     CDS             complement(3625..4047)
                     /locus_tag="ACHOQQ_RS21910"
                     /old_locus_tag="pgaptmp_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4472547.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKAEATEPVTSKKHSKAS"
     gene            complement(4063..>4764)
                     /locus_tag="ACHOQQ_RS21915"
                     /old_locus_tag="pgaptmp_004383"
     CDS             complement(4063..>4764)
                     /locus_tag="ACHOQQ_RS21915"
                     /old_locus_tag="pgaptmp_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4472548.1"
                     /translation="LWVYTGKGDAYANPLSDDEWTRLAKIKDLTPGEMTAESYDDNYL
                     DDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDSSETRAYKIRFPNGTVD
                     VFRGWVSAIGKSVTAKEVITRTVKITNIGRPSLAEDRGEITPVTGITVTPLTGNVAKG
                     QNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKGVAAGKAQIPVVTGNGE
                     FAAVAEITVTDGAAG"
BASE COUNT          926 a         1594 c         1123 g         1121 t
ORIGIN      
        1 gccatcccgg tgaccagccc gaaaagattg gacactgtca gcgacgggcg ggcactgctg
       61 ccctttccgt tcatctcaaa gccgctgccc tcaatcgggt acgcctgata ttcacgcccc
      121 tgccaggtca ctggctcccc tttttcattc agctcattgc agaaaaaata ccgctcaccg
      181 ccctgcaccg tcaggtcgat ttcccagagc accacccgcg gtgactgctc tgatttaacc
      241 gactcgttca ggctttcttc gtgaatatcc tgcatcagtt caccacctgc ttaaactccg
      301 cgctgaactc aacgcgcaac atcccgaccc gcgcagacca cccggcacag gtcaccttta
      361 tctgccggta tgcatagggt ggcttccaca aaaatgcctt ccagcccccg tgctctgcca
      421 ggaacgcttc cagatgccgg gcctcctccc gggtcacgga aagcgtcacc ctgtatgttt
      481 tcaggtcagc attcagccct gccgccatac gctgtgagta cccgtcacca aaacgcactt
      541 cacgcaccga cggctgcgag ttcacctcca tatccggctt cactttccag cgaaaggttt
      601 tcatcgcccg ctccctgata acataccgcc atcacgcaac tgcagccgga gctcatcctg
      661 tgccccttta cgggccatgt catacaccgc cttcatcagc tgcggccccg cctgtccgtt
      721 gataccgtcg ttctgaatca ccacgtgatt gttctgatta aaattaatac cttccgcccg
      781 ccgcatctgc gccggacttc cggcaccgcc gacataacca ccttccgcat agccgcgcat
      841 caggcggtaa agattcccca cacctatccg gctggttgcc tctttcgtga aaacaaactc
      901 cccgcggtga actatccccg caggctcata tttgccgccc gtccccgtaa atccgccggt
      961 cgcaaaatga aagttcgccg ccgcagcctc aatggccgtt cccgtggaag cggatgcgcc
     1021 accaccgaaa gcaccaccaa tggcgctgcc gatacgcccg acaattccca ccatggcctg
     1081 tttaagcagg atttctgtca tcatggacag caccgaacgg gtgaatcccc gccagtctgc
     1141 ctctgcaccg gtcagcatcg ccgccatatt ctgtgcaata ccgtcaaagg tctgcgtggc
     1201 agcactttta acctgcgaaa aactgtccgt cgcactttcc gtccactccc cccagccgga
     1261 cttcagcccg gccagccagt caccgcgcag catgtcttca tccgcccatg tctgtttcag
     1321 tgccccggtg acctgtgcca gcgcctgcgg attatcgcca tacacgtcac gaagacgctg
     1381 ctcttcagac tcccgctgcg cctgacggtc agtgagacca cgggcttttg cgctgatggc
     1441 ggcctgcttc gcgctctgct gctcttcaaa ccgtgccgcc tgctgtgcca gctcattcag
     1501 gcgtttctgg tattcaacct tgtcgcccag ctcagccagc tggcgtttgt actccagcgt
     1561 ctctttctca tgggccagca gggatttttc ctgcccggat aactgccgtt tcgtggcggc
     1621 ctctttcagg accgcatact gactttccgc tttccataaa tcacggcgct gctggctgat
     1681 tttctcattc gcaccgctgt gcttctccag cgtcctgagc tcagcttcaa gggcaagcag
     1741 agcctctctt gcctgctcct cttccctctc cccggcagag cgcgttttcg gtgatgtatg
     1801 ctttttacct gtcagctctt cagccagacg gctgacggct tcctgctgcc ccggaccttt
     1861 gctgacgcct gttgcacgcg agcggttgat gtaccccatt tccccctggc gtatacgcgc
     1921 atcccgttcc gcaatggatt ttctcagcgc cagttcatcg cgttttgttt tctcaataaa
     1981 ttcccggttc tcttctgcca gctcgccaaa cagcgcactg acgccgggga catcttttgt
     2041 cgtttcccag gctgactgaa taaattcagc cagcgccaga tccccctgca caagcagcag
     2101 cttcacctgt tcaacggttc ctgccaccac gtcggtgatc agactgaggg cccccagagt
     2161 atgatcacct atccatgccc atgcgtcaga agtccaggtt ttaacatcgt cccagatttt
     2221 ttccaccggc gtggccgctt tatcaagttg ctccagacgt gcattcatga catccgcaaa
     2281 cagggacatc gcctccgtta ccgcagcctg tttacctttc gtgcgctcaa gctcatcaat
     2341 atggcgtaac tgggaaacgc tcaggaagtt atactgctga ttcagggagg ccagcgcctt
     2401 caccggatct gctgcaatcc cttcaaaggc tttttccacc ttcccggcat cgtcccccac
     2461 cgtctgcagc catctctgag aggtttcccc catgatccgt agctgcccgg cggtatattt
     2521 cccgctttct gccagacggg ccagattttc tgccgcctgt ctgataccac caccggcttc
     2581 atcgctgatc accccggcca ttttccacaa ttctgccgtt gtggtggcag ccgcccctcc
     2641 ggtcagggtc agtgaacgca acaaggcccg gtcagcctgc tctgcctgcc aggcggcggc
     2701 agcaagcgcg gccagtacgg caaccccgcc acctgccgcc acacgggcca ccgacataaa
     2761 tcgtcccagt tcaccggcat tccgggcatt ttcagccagt gcatttgccg tatctgacag
     2821 cgaatcctct gatgattcag aggcatccct gatcccgaga agttcctcct tcagcagggt
     2881 aagcaggctg agcggtccac cgaatgaatc gctgatctgc cccccctgct gcagcatgat
     2941 aaggaaggga ttctgaccac cggcaagctg agtgacaata tccgtgaact gtgcgggcag
     3001 tgtgcgcatg gcagccttat actgtccgac tgatatcccg gctttttgtg cagccagcgc
     3061 ctgtcggctc agactctgct cagcagcatc agcctgtttt ctgaaagact gactgacttt
     3121 cccggacatc aaatcagcaa gcgcactggt ttcacccagc tcttttttta cccgcgctgc
     3181 ctcttcagaa aaacgggcag aatccagtgt cagcacggct gtcagatcgg caaaattacc
     3241 tgccatcgtg gtcacctcct gaaatgtcct ctgatgccat caataactgt cacaacctcc
     3301 ttccctcctc cccgaaacgg actccaccgg cgaggcccgc cgccttctgc atcagtacat
     3361 cattttcgtc cggcgtctcc gcctgccctt cctctgcctc cggagcgaac aggctgaaat
     3421 ccgccggatg catatccgga tcgcaaaaaa acaggctgag tacggcgtac gtcagcccgg
     3481 aaaaatgcat atccagctgg gtatcctgaa aataatgcgt gcagtaaaaa cgtcgccagt
     3541 cggcatattc ggtggatgtc atcccggcaa gcatggcgcg ccagtcggct ctccccatct
     3601 cacgcgccag tttcaggaca aagttcagct cgccttcgaa tgcttttttg atgttaccgg
     3661 ctcagtcgct tctgctttcc cggttggttc aggatcggca tcgtgccggt tatccagcat
     3721 acctgaaaga taaagcaccc ggttcgttgc ctgattcagt gcatcagcag gccatcccag
     3781 catcacttca cggcggatct gctgcatctc tgtctccgga gaggccagag tgcctttgag
     3841 agaatgggaa tgccatagcg acatcgccac aagcagggat gccgtttcca gatatcgctg
     3901 gttaatgtgc acggcatcat gctccgttgt ctcctgttgt gctgcgtctg aaacaaactt
     3961 catataatca aaccgctgca gcgcagacag ctccgacagc gtgacggaca ccccgttata
     4021 ttcaaattgt tctgttttca gaaacatgct ttatctcccc cctcagcccg cagcgccatc
     4081 cgtgacggtg atctccgcca ccgccgcaaa ctcaccattg ccggtgacaa cagggatctg
     4141 cgctttacct gccgcaacac ctttcacggt gatcgtgttc cccttcacgg taatggtggc
     4201 gaaattctga ttcgctgagg ttgcgcggaa cgttttatcc gttgcgcctt ccggctgaac
     4261 ggccacggtc agggtgatat tctgaccttt tgccacattc cccgttagtg gcgtcacggt
     4321 aataccggtg accggtgtga tttctccccg atcctcagcc agcgacggac gaccaatatt
     4381 ggtgattttc acagtgcggg tgatcacttc tttggcggtc acggatttac caatggcgct
     4441 cacccagccc cggaacacat ccaccgtgcc gttcgggaag cggattttgt aggcccgggt
     4501 ctcgctgctg tcaaaccagg caatcaaatc acgctgccct ttctcgccgg gcttccaggc
     4561 cagcgtaaaa ctggtgtcac cggcggattt ctgcccctgc ccggtggata cccagtcggc
     4621 atcctcatca tccagatagt tatcgtcgta ggattccgcc gtcatctcgc cgggggtcag
     4681 atcctttatt tttgccagac gcgtccactc atcatctgac agcgggtttg cataagcatc
     4741 gcccttaccg gtgtaaaccc agag
//
LOCUS       DAZKFU020000305         4663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZKFU020000305 DAZKFU020000000
VERSION     DAZKFU020000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.307"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..928
                     /locus_tag="ACHOQQ_RS21920"
                     /old_locus_tag="pgaptmp_004384"
     CDS             <1..928
                     /locus_tag="ACHOQQ_RS21920"
                     /old_locus_tag="pgaptmp_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGU4472549.1"
                     /translation="CLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVL
                     NLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEG
                     SYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLII
                     DNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEGQNFSGL
                     RQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSL
                     NEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1137..2102
                     /gene="lipA"
                     /locus_tag="ACHOQQ_RS21925"
                     /old_locus_tag="pgaptmp_004385"
     CDS             1137..2102
                     /gene="lipA"
                     /locus_tag="ACHOQQ_RS21925"
                     /old_locus_tag="pgaptmp_004385"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGU4472550.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAAALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2203..2406)
                     /gene="tatE"
                     /locus_tag="ACHOQQ_RS21930"
                     /old_locus_tag="pgaptmp_004386"
     CDS             complement(2203..2406)
                     /gene="tatE"
                     /locus_tag="ACHOQQ_RS21930"
                     /old_locus_tag="pgaptmp_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGU4472551.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2535..3323)
                     /locus_tag="ACHOQQ_RS21935"
                     /old_locus_tag="pgaptmp_004387"
     CDS             complement(2535..3323)
                     /locus_tag="ACHOQQ_RS21935"
                     /old_locus_tag="pgaptmp_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGU4472552.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILALPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3416..3799
                     /gene="crcB"
                     /locus_tag="ACHOQQ_RS21940"
                     /old_locus_tag="pgaptmp_004388"
     CDS             3416..3799
                     /gene="crcB"
                     /locus_tag="ACHOQQ_RS21940"
                     /old_locus_tag="pgaptmp_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU4472553.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3853..4062)
                     /gene="cspE"
                     /locus_tag="ACHOQQ_RS21945"
                     /old_locus_tag="pgaptmp_004389"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3853..4062)
                     /gene="cspE"
                     /locus_tag="ACHOQQ_RS21945"
                     /old_locus_tag="pgaptmp_004389"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4472554.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4237..>4663)
                     /gene="pagP"
                     /locus_tag="ACHOQQ_RS21950"
                     /old_locus_tag="pgaptmp_004390"
     CDS             complement(4237..>4663)
                     /gene="pagP"
                     /locus_tag="ACHOQQ_RS21950"
                     /old_locus_tag="pgaptmp_004390"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGU4472555.1"
                     /translation="EHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKG
                     NWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLANENFHLGLGFTAGVTARDNWNYIPL
                     PVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1216 a         1195 c         1061 g         1191 t
ORIGIN      
        1 ctgtttaagc agaaagtcgt cagaaggtaa accacaaata tttacaacct tacgaaatat
       61 tgatcttaac cttctgacta tttttgaagc tgtatatgta cataaaggga tcgttaatgc
      121 agcgaaagtg cttaatctga ccccctcggc aatcagtcag tctatacaga aactgcgcgt
      181 tatattccct gacccattgt ttattcgcaa aggccagggt gtcactccta ccgcatttgc
      241 gatgcatcta catgagtata tcagtcaggg ccttgaatcc attcttggcg cgctggatat
      301 cgaaggaagc tatgataagc aacgaacgat aactatcgcg accaccccct cggtcggagc
      361 cctggtcctt cctgtcattt atcgggcgat taaaactcac tatccgcagc ttttactgcg
      421 caacccgccc atcagcgacg cggaaaacca actcagtcag tttcaaaccg atctcatcat
      481 cgataacatg ttttgcacca atcgtacggt gcaacatcat gttctgttca ccgacaatat
      541 ggtgttaatt tgccgcgagg gaaatccact actctcttta gaagatgaca gagagactat
      601 cgacaacgct gcacatgtac tcctgttacc ggaaggacaa aatttcagcg gtctgcggca
      661 gagaattcaa gagatgtttc cggaccggca gatcagcttc accagctaca acattttgac
      721 aatcgctgca ctggttgcca acagtgacat gttagcgatt attcccagcc gtttttataa
      781 cctgtttagc cgttgctggc cgctggaaaa actgcctttt ccgtccttaa atgaggagca
      841 aatagatttc tctatccact acaacaaatt cagcctgcgt gatcccatcc tgcacggagt
      901 aatagatgtt atccgtaatg cattttgaaa aagtattgtt acagccagat cacagcgcag
      961 aacgataaaa tggcacaatc caacaacaat tttacatttt cgcgaccgct ttggctgctt
     1021 tcaggtccgt ttcaatgata tactgccagt cgttaattca aaaatagttg ataattacaa
     1081 caatctattg aattgaaacg ctttccttcg taattcgcaa ctggaacacg cacgctatga
     1141 gtaaacccat tgtgatggaa cgcggtgtta aataccgcga tgccgataag atggccctta
     1201 tcccggttaa aaacgtggca acagagcgcg aagccctgct gcgcaagccg gaatggatga
     1261 aaatcaagct tccagcggac tctacacgta tccagggcat caaagccgca atgcgcaaaa
     1321 atggcctgca ttctgtctgc gaggaagcct cctgccctaa cctggcggaa tgcttcaacc
     1381 acggcacagc aacgtttatg atcctgggcg ctatttgtac ccgccgttgt ccgttctgtg
     1441 acgttgccca cggtcgcccg gtagctcctg atgccaatga gccagtgaaa ctggcgcaga
     1501 ccattgccga tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt gatgacctgc
     1561 gcgatggcgg tgcccaacac tttgcggatt gtattactgc cattcgggaa aaaagcccgc
     1621 aaatcaaaat tgaaactctg gtgccggatt tccgcggtcg tatggatcgt gctctggata
     1681 ttctgactgc aacgccccca gatgtgttca accataacct ggaaaacgta ccgcgtattt
     1741 accggcaggt acggcctggc gcagattaca actggtcgct gaagctgctg gaacgcttta
     1801 aagaagcgca tccggaaatc ccgaccaagt ctggtctgat ggtgggtctg ggtgaaacca
     1861 atgaagaaat tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg atgttaacgc
     1921 tggggcaata tttgcagcca agccgccatc acctgccggt tcaacgttac gttagcccgg
     1981 atgagttcga cgaaatgaaa gccgcagcgc tggcgatggg ctttacccat gctgcatgcg
     2041 gtccgtttgt ccgctcttct taccacgccg atttgcaggc gaaagggatg gaagttaagt
     2101 aatcctgtaa taattactta catctgtctg ataaaaaacc cgctttttga agcgggtttt
     2161 ttgtatcgaa caagatattg agggagcgtc ctgctcgcca cgtcactctt tatgagagag
     2221 cttttcagcc tgaagatcaa cgtctgcgcc ttttttcgcc gcagcatcgt catcattcat
     2281 cgccttcttg aaccctttaa tggccgctcc aaggtctccg cccagcgtac gtaacttctt
     2341 agtcccaaac agcagaacga ccagcgccgc aactaccagc agtttggtaa tactaatctc
     2401 acccatagat accttcttga catataaccg gcaacagaac gcgccttaaa aaccagagaa
     2461 tattaacggt cattcgacgc gcgcacacaa tagcaatctg taacaaggtg aatcaagcgc
     2521 cgagtaaaaa aacatcataa taattgcggc ggcgcaaagc gacggttgtt taagacgggc
     2581 agttgcgcgc gcacctgacg cacacgttcg ggcgtcactt ccgccataat gagtgcaggc
     2641 atttctgacg ctgccgcaat ggtgacgcca aagggatcta taatccggct ttgcccgata
     2701 tttttgttcc cgcactcccc cgccgccacc atataacagg tggtatccag cgcacgagcg
     2761 gcaagcaacg ttgaccagtg atgctctttg agcggcccgc gaacccaggc ggcaggaagt
     2821 gccaggattt cagctccctg taatgcctgt gccagcgcca gctctggaaa gcgtaagtca
     2881 taacaggtca tcagaccgac cttcatccct tccacctcca gtaacggagc gatttcatta
     2941 ccagcatcaa cacggcgtga ttcctgaatg gcaaatgcat catagagatg cagtttggca
     3001 taacgggcga caatgtttcc tgcctgtagc gccaccagca tattccatgc gcgccccggc
     3061 gttgaaggaa catgaatcgt cagaattgtc gtcatcatgt tacgtttact ttctcgccgt
     3121 aaacgtccga ggaattcgcc ttccagcagc tgtgctgatt taaccgatag atctgcatca
     3181 tgatcatcgc gtgccagcaa tgcttccggc aggacaaaca gcgatacgtc gttttccgcc
     3241 gcctgcgcca tcaacgaggc acaaatctca gcgttctttt cccacacaga tgtaacagca
     3301 aactgtcctg cagcaaccag cattgccacc tcctgtcaga gtcgtaaaat tcgccaacga
     3361 cgattattcg gcgttacact tatcactcat acaaatcaaa tagcaggatt ttgcagtgtt
     3421 acaacttctt ttagcagttt ttattggcgg tggtacggga agcgtggcga gatggctgtt
     3481 aagtatgcga tttaacccgc tgcatcaggc gattccgttg gggacgctgg ctgcaaatct
     3541 gattggggca ttcatcatag gaatgggatt cgcctggttc agcaggatga caaacattga
     3601 tcccgtgtgg aaagtattaa tcaccaccgg attttgtggc ggtcttacaa ccttctcaac
     3661 attttcggca gaagtggtgt ttttgttaca agagggccgc tttggctggg cgttactgaa
     3721 cgttttcgtc aaccttctgg ggtcttttgc catgaccgca ctggcattct ggctgttttc
     3781 ggcctcaacc gcacactaaa gaccaaaaaa aacccgctga ttaagcgggt tttgaattct
     3841 tgctgacgta tcttacagag cgattacgtt tgcagcagaa gggcctttgg caccgttagt
     3901 gatttcgaac tctacgcgct gaccttcagc aagagtttta aaaccattag tctggattgc
     3961 agagaagtgt acgaacacgt ctttgctgcc gtcttccgga gtaatgaaac cgaatccttt
     4021 ggactcatta aaccacttaa cgttaccttt aatcttagac atcaaaatta cctttacatg
     4081 aaaaatagac acaaatgctg tgtcggttac cagtacacca attgtggtac gctttgtcca
     4141 gtgcaacttt actaaaaagt gataaaagtc gcgttatttt tgatgttaat tattttagtt
     4201 attgatttta aagaagttac taaaacttca tttgtctcaa aactgaaagc gcatccaggc
     4261 aaagtacaca ttgccattgt tgtaggtacc cggaatgtag gtcatctgaa aagtcactgg
     4321 gccataaccc acggaggcca atggcagtag aaccgggaga gggatgtaat tccagttatc
     4381 acgtgccgtt acgccagcgg tgaatcccag acctaaatga aaattttcat tcgccagcgg
     4441 tcgccaggta ctttcccatc cgtatccggc aatcggttcc catttgttcc acgagtcctt
     4501 aaatgccatg gcatacaggc catgccagtt tcctttttca tcccaacgcg acaggccaaa
     4561 accgccaccc cacggtcgct cgttatagcg atcggttttt tctttgtcgt aagcgaaacg
     4621 tgcatgccag gtgatggcag gaatatataa atcataatgt tca
//
LOCUS       DAZKFU020000306         4631 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZKFU020000306 DAZKFU020000000
VERSION     DAZKFU020000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.308"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..617)
                     /locus_tag="ACHOQQ_RS21955"
                     /old_locus_tag="pgaptmp_004391"
     CDS             complement(<1..617)
                     /locus_tag="ACHOQQ_RS21955"
                     /old_locus_tag="pgaptmp_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4472556.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERA"
     gene            complement(732..807)
                     /locus_tag="ACHOQQ_RS21960"
                     /old_locus_tag="pgaptmp_004392"
     tRNA            complement(732..807)
                     /locus_tag="ACHOQQ_RS21960"
                     /old_locus_tag="pgaptmp_004392"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(772..774),aa:Thr,seq:ggt)
     gene            complement(814..888)
                     /locus_tag="ACHOQQ_RS21965"
                     /old_locus_tag="pgaptmp_004393"
     tRNA            complement(814..888)
                     /locus_tag="ACHOQQ_RS21965"
                     /old_locus_tag="pgaptmp_004393"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Gly,seq:tcc)
     gene            complement(1005..1089)
                     /locus_tag="ACHOQQ_RS21970"
                     /old_locus_tag="pgaptmp_004394"
     tRNA            complement(1005..1089)
                     /locus_tag="ACHOQQ_RS21970"
                     /old_locus_tag="pgaptmp_004394"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1053..1055),aa:Tyr,seq:gta)
     gene            complement(1098..1173)
                     /locus_tag="ACHOQQ_RS21975"
                     /old_locus_tag="pgaptmp_004395"
     tRNA            complement(1098..1173)
                     /locus_tag="ACHOQQ_RS21975"
                     /old_locus_tag="pgaptmp_004395"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1138..1140),aa:Thr,seq:tgt)
     gene            1535..2485
                     /gene="coaA"
                     /locus_tag="ACHOQQ_RS21980"
                     /old_locus_tag="pgaptmp_004396"
     CDS             1535..2485
                     /gene="coaA"
                     /locus_tag="ACHOQQ_RS21980"
                     /old_locus_tag="pgaptmp_004396"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGU4472557.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2514..3479)
                     /gene="birA"
                     /locus_tag="ACHOQQ_RS21985"
                     /old_locus_tag="pgaptmp_004397"
     CDS             complement(2514..3479)
                     /gene="birA"
                     /locus_tag="ACHOQQ_RS21985"
                     /old_locus_tag="pgaptmp_004397"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGU4472558.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3476..4504)
                     /gene="murB"
                     /locus_tag="ACHOQQ_RS21990"
                     /old_locus_tag="pgaptmp_004398"
     CDS             complement(3476..4504)
                     /gene="murB"
                     /locus_tag="ACHOQQ_RS21990"
                     /old_locus_tag="pgaptmp_004398"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGU4472559.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGRVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1167 a         1170 c         1059 g         1235 t
ORIGIN      
        1 gcacgctctg gttccggaat gtaagaatcc aggaagccag ccagttccag gattttcgct
       61 tcccactctg cgtcgccttc cagcgctttc agagcagaac cacgaacgat cggagtgtcg
      121 tcgcccggga agtcgtactg agacagaagt tcacgaactt ccatttcaac cagttccagc
      181 agctcttcgt catcaaccat gtcacatttg ttcaggaaca cgatgatgta cggaacgcct
      241 acctgacgac ccagcaggat gtgctcacga gtctgcggca tcgggccgtc agtcgcagca
      301 actaccagga tcgcgccgtc catctgagca gcaccggtga tcatgttttt aacatagtcg
      361 gcgtgccccg ggcagtctac gtgtgcgtag tgacgggtcg gggtgtcgta ttcaacgtga
      421 gaagtgttga tggtgatacc acgagctttt tcttccggcg cgttatcgat ctggtcgaat
      481 gcgcgagcag caccgccgta ggttttagcc agtacggtag taattgcagc ggtcagcgtt
      541 gttttaccat ggtcaacgtg gccgatagta ccgacgttaa cgtgcggttt tgtacgttca
      601 aacttttctt tagacatcga ttgtccctct aagacacgga taaatcggtg atatcaccac
      661 atcaaccagg caacatgccc gacttgttga atgcaataaa cagaaggaaa aaacagggag
      721 gagaaaagaa gtggtgctga taggcagatt cgaactgccg acctcaccct taccaagggt
      781 gcgctctacc aactgagcta tatcagcaca tcttggagcg ggcagcggga atcgaacccg
      841 catcatcagc ttggaaggct gaggtaatag ccattatacg atgcccgcat cctggaactc
      901 ggctacctga ttttcattct gcactgaata tcgagagaag ctctctttat tcgagccggt
      961 aagcgaactt atcgtctcgg gctacgccat cgcgtggccg aaattggtgg tgggggaagg
     1021 attcgaacct tcgaagtctg tgacggcaga tttacagtct gctccctttg gccgctcggg
     1081 aaccccaccg gacttgatgg tgccgactac cggaatcgaa ctggtgacct actgattaca
     1141 agtcagttgc tctacctact gagctaagtc ggcatcaagt agcgcgcatt ctatggagac
     1201 atgcgagttc atgcaactaa aaaattgcat aatttgtttt attggtcaca ttttatgcga
     1261 cacgatgaag aaacagccga atagagggca gattagcaca ctttttcaac atcattgtgc
     1321 tcaacaatgc gctcctgcta aaccataatt ctttttatca gatggaatat ctgtcacatt
     1381 gcttttcaac gatagcttcc tgggagagat tttttcttat tattcctccc catctggtgt
     1441 taccctcctg cccattaacc cattcaacag aactgtgacg cgccatggca aatatcgctt
     1501 tgccgataga gctatgaccg ccagaaacat gcttatgagt ataaaagagc aaacgttaat
     1561 gacgccttac ctacagtttg accgcaacca gtgggcagct ctgcgtgatt ccgtacctat
     1621 gacgttatcg gaagatgaga tcgcccgtct caaaggtatt aatgaagatc tctcgttaga
     1681 agaagttgcc gagatctatt tacctctgtc acgtttgctg aacttctata taagctcgaa
     1741 tctgcgccgt caggcagttc tggaacagtt tcttggtacc aacgggcaac gcattcctta
     1801 cattatcagt attgctggta gtgtcgcggt ggggaaaagt acaaccgccc gtgtattgca
     1861 ggcactatta agccgttggc cggaacatcg tcgtgttgaa ctgatcacta cagatggctt
     1921 ccttcaccct aatcaggttc tgaaagaacg tggtctgatg aagaagaaag gcttcccgga
     1981 atcgtatgat atgcatcgcc tggtgaagtt tgtttccgat ctcaagtccg gcgtgccaaa
     2041 cgttacagcc cctgtttact cgcatcttat ttatgatgtg atcccggatg gagataaaac
     2101 tgttgttcag cctgatattt taattcttga agggttaaat gtcttacaga gcgggatgga
     2161 ttatccacac gatccacatc atgtatttgt ttctgatttt gtcgattttt cgatatatgt
     2221 tgatgctccg gaagacttac ttcagacgtg gtatatcaac cgttttctga aattccgcga
     2281 aggggctttt acagacccgg attcctattt tcataactac gcgaaattaa ctaaagaaga
     2341 agctattaag actgccatga cgttgtggaa agagatcaac tggctgaact taaagcaaaa
     2401 tattctacct actcgcgagc gcgccagttt aatcctgacg aaaagtgcta atcatgcggt
     2461 agaagaggtc agactacgca aataatttgc aggggagcta atgctcccct ttcttatttt
     2521 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc gtcctgctca
     2581 agcaataaag cgccttgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     2641 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgaaagataa
     2701 ggtgccaatc cttcttgttc gaagagttcc agcgccgcgc gtaattcacg tattagcatg
     2761 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     2821 ttaacgacac tctcttcaac acgacgcatt gccatgttga tcccggctcc aatgactatt
     2881 tgcgccgcat cgccggtttt gccagtcagc tccacaagaa tgcctgccag cttgcgatcc
     2941 tgcagataga ggtcattagg ccatttgaca cgaactttat ctgctcccag cttgcgtaat
     3001 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     3061 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccgc
     3121 ccccggcgac cacggccagc ctgctggtat tctgcaacgc aggcatcgcc cgatttaagc
     3181 tctccgatac gatcaagaag gtactgattc gtggagtcaa taactggcag cacggctaca
     3241 ctaccgccat ccagctgacc caatatctgt tcagcattaa gtaactggat gggctcaggc
     3301 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     3361 atgtgtttat taatagccgc ccggctcatt cctaacgttt cacccaactg ctcacctgag
     3421 tgaaattcgc cattcgctaa cagcgcgatt aatttcagtg gcacggtgtt atccttcatg
     3481 aaattgtctc cactgcgctc acttcacctg atgcaccaat aaagcggact tcaggctcaa
     3541 gccagacatt aaatttttca cccacttttt gtcttacatg gtgtgccagt tgcaccacat
     3601 cttcgctttt tgcattgtct tcattaatga gaactaacgc ctgttgacgg tgcaccgcag
     3661 ccccacccat ttgcatccct tttagctggc actgatcgat aagccaacct gctgccagtt
     3721 ttactgaacc atccgcctgg gggtaatttg gtgctgttgg aaattgtgac agtaatgctt
     3781 tagccgtttc agcagataca acagggtttt tgaagaaact accggcattg ccattcactt
     3841 ttggatcagg aagtttggtg gtgcgcatat gacacaccgc atcaaatact tgctgtggcg
     3901 ttactgttgt aggatccaga cgagttaagt caccatacgt tagtacaggt tgccactctt
     3961 ttggcagacg cagacctacg gctacaatgg cgaagcggtc ctggtattca tgtttaaaaa
     4021 tactgtcgcg atagccaaaa cggcactctt tggcagttaa gcgcacttgc ttgcctgtcg
     4081 ccagttcaac acaatcaaca taagcgcaaa ctcgctgtaa ttctacgcca taagcaccaa
     4141 tattctggat aggtgatgag ccgacacgac caggaattaa tgccagattt tccagaccag
     4201 gcataccttc ctgcaaagtg tattttacca ggcgatgcca gttttctccg gctcctacat
     4261 gtaaatacca cgcatcaggt tcatcatgaa tttcgatacc tttgatccgg ttgatgatca
     4321 ccgtgccgcg atagtcttcc agaaaaagta cattacttcc ttcacccaga ataagaacgg
     4381 gttgcccttc tgcggttgca tgctgccagg cattgagtag ttgttgttcg tcttcggcac
     4441 atacaatgtg ctgagcatta tgatcaatgc caaatgtgtt ccagggtttt aaggagtggt
     4501 tcatagctgc tttcctgatg caaaaacgag gctagtttac cgtatctgtg gggggatggc
     4561 ttgtagttat gacgacaggg agagtttgta gaaacgcaaa aaggcccagt cttccgaccg
     4621 ggccttctgc t
//
LOCUS       DAZKFU020000307         4624 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZKFU020000307 DAZKFU020000000
VERSION     DAZKFU020000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.309"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..1231)
                     /locus_tag="ACHOQQ_RS21995"
                     /old_locus_tag="pgaptmp_004399"
     CDS             complement(5..1231)
                     /locus_tag="ACHOQQ_RS21995"
                     /old_locus_tag="pgaptmp_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4472560.1"
                     /translation="MKDMQAMLPQTFYIKPGQFIWSYLGTFLFMLGGCIENSWLSAWL
                     NTQGFDQSQIGQIFAAYGIVVAVTSWLSGVCVDMFGPRKVMLAGFSVYLIASIAFLNF
                     AIPSHDFSTILISYMLRGVGYPLVCYSFLVRLTIQLDNHQQGIGTSLFWVVYNLGFTI
                     IGPVVAASLIPVLGHINVMWAGIGVALLGGIFMLVFERNAFILKPRTTSVFKELSAGI
                     SIMYQRPRIGLAVIVKTINGLGTYGFVVVLPLFLLDKQFTLEEWASIWGITFISNQIF
                     NIIFGWMGDKIGFRRTIQIFGSLLTGFATLIVYWVPIVWGHNYVAFMLAMCLWGAGLA
                     GFVPMTPLVPMMAPDKKGAANSAVNFGSGLGNFVGPALVSILTGFGTGVVMYTMAGLY
                     LFSGILVQFLKVPGEK"
     gene            complement(1304..2326)
                     /gene="iolG2"
                     /locus_tag="ACHOQQ_RS22000"
                     /old_locus_tag="pgaptmp_004400"
     CDS             complement(1304..2326)
                     /gene="iolG2"
                     /locus_tag="ACHOQQ_RS22000"
                     /old_locus_tag="pgaptmp_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-chiro-inositol-2-dehydrogenase IolG2"
                     /protein_id="HGU4472561.1"
                     /translation="MQKLRCGIIGLGRVGKMHVENMYLLPQLDIVCAADSFIEEMSDW
                     LSSVNITSGYKDYQEMLQRDDIEAVFIFTSTDMHEEIVTAAVQAGKHIFCEKPLSMRE
                     DEKASLAVLRAVKEKGVKLQIAFNRRFDPQFREVYELVRRGKIGQPQMIKITSRDPEL
                     LPHDLIKRIGGLIFDFTMHDFDMARFIMQDEVSEVYVKGNTLIDPTLKNIDDVDTLVV
                     MLTFRNGGYALIDNSRRAVYGYDQRVEVFGSAGMAYADNVSESAVKVYNSQNCIMKNP
                     LPDFALRYREAYRTEILHFIDSVLNHTPIVCTGEDALLAQRIAIAAKQSLKSGLPVKI
                     KTDIQL"
     gene            complement(2499..4439)
                     /gene="iolD"
                     /locus_tag="ACHOQQ_RS22005"
                     /old_locus_tag="pgaptmp_004401"
     CDS             complement(2499..4439)
                     /gene="iolD"
                     /locus_tag="ACHOQQ_RS22005"
                     /old_locus_tag="pgaptmp_004401"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HGU4472562.1"
                     /translation="MKTIRLTMAQALVRFLDNQYIEVDGREIKFVKGIFAIFGHGNVV
                     GLGQALEEECGQLSVHQGRNEQGMAHIATGFARQMRRQQIYACTSSVGPGAANMITAA
                     ATATANRIPLLLLPGDVYASRQPDPVLQQIEQEHDLTLSTNDAFRAVSRYWDRIIRPE
                     QLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHVPTELM
                     LNDAVALILRHQRPIIICGGGVKYSQSEQELLRFAEQYHLPIAETQAGKGALSSAHPL
                     NVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDV
                     FKLDGVQLQADARVALTQLCERLAPEGYISQWGKTIHRVRSQYLAEVDRVYAAEYSGK
                     DFKPEIVDHMDTQKVFEEFNEITHSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQ
                     RIWQSRGDNDYHVEYGYSCMGYEVNAALGAKLAQPEREVYSFVGDGSFMMLHSELVTS
                     VQMGKKINVILLDNMTNGCINNLQMEHGMDSFFTEFRFHKQDNGRQEGGLIPVDFARI
                     AEGYGCKSYRVTSIGQLLEALEDARKQTVSTLIDIKVLPKTMAHKYLSWWRVGGARVS
                     RSERIQAVARMLDEHIGQARQY"
BASE COUNT         1374 a         1244 c          877 g         1129 t
ORIGIN      
        1 ttatttattt ctcaccagga actttaagaa actgaaccag gatgccacta aataaataga
       61 gtcctgccat agtatacata accacgcctg taccaaaacc tgtcagaatg gataccaacg
      121 caggtccgac aaaatttccc aggccagaac cgaaattcac cgctgagttt gctgccccct
      181 tcttatccgg tgccatcatc ggtaccagcg gcgtcatcgg cacaaatccc gccagccccg
      241 ctccccacag gcacattgcc agcataaaag caacgtaatt atgcccccac actatcggca
      301 cccagtagac aatcagcgtg gcaaacccgg tcagaagact gccaaaaatc tggatagtac
      361 ggcgaaaacc tattttatcc cccatccagc cgaaaataat gttgaaaatc tgattagaaa
      421 taaaggttat accccagata ctcgcccact cctcaagggt aaactgttta tccagcaaaa
      481 acagcggcaa caccactaca aaaccatagg tgccgagtcc attgattgtt ttaacaatca
      541 ctgccagacc aattcggggt cgttgataca taatggaaat acctgccgac aactctttaa
      601 agactgacgt agttcttggc ttcagaatga acgcattgcg ctcaaagacc agcatgaaaa
      661 tccccccaag cagtgcaacc ccaatcccgg cccacataac attaatatgc ccaaggacag
      721 gaattaacga agcagctact accggaccaa tgatggtaaa acctaagtta tatactaccc
      781 aaaacagcga cgttccaatt ccctgttgat gattatcgag ttgaatggtc agtcggacaa
      841 gaaaggaata gcagaccaac ggataaccta ctccccgcag catatagcta atgagaattg
      901 tactaaaatc atggctggga atcgcaaaat taagaaacgc gatagaagcg atcaggtaaa
      961 cggagaaacc agcaagcata acctttctgg ggccaaacat atccacacag acaccggata
     1021 accaggaagt tacggcaacc acaattccgt aggcggcaaa tatctgtccg atctgagact
     1081 gatcaaatcc ctgagtattc agccaggcag atagccaact attttcaata cagccgccca
     1141 acataaataa aaatgttccc aggtagctcc agataaactg acctggcttt atataaaatg
     1201 tctgaggcaa cattgcctgc atatctttca ttttataacc ctcagtacgg ataatctctc
     1261 gctcgtaata gtgcgagaga cccttcgcta tttacaaatg cagctaaagt tgaatatcag
     1321 tttttatctt cactggcagt ccacttttta atgattgttt tgcagcaatg gcaatacgct
     1381 gcgccaataa tgcatcttca ccagtacaga caatgggtgt atgattcaga acagaatcaa
     1441 taaagtgtaa tatttccgtt ctataagctt cacgatagcg tagcgcaaaa tcaggcaaag
     1501 gatttttcat gatacagttc tgactgttat ataccttcac cgccgattca ctgacattgt
     1561 cggcatacgc catccctgct gaaccaaaga cctcaacccg ttggtcgtag ccataaaccg
     1621 cccgacgact attatcaatt agcgcataac caccgtttct gaaggtcagc atcaccacga
     1681 gagtatctac gtcatcaata tttttcagag taggatcaat caatgtattg cctttaacat
     1741 aaacctcgct aacttcatcc tgcataataa agcgagccat atcaaagtca tgcatagtaa
     1801 aatcaaatat gagtccacct atcctcttga tcaaatcgtg cggcaacaac tctggatcac
     1861 gtgaggtgat ctttatcatc tgaggctgac caatcttgcc tctgcgcacc agttcataca
     1921 cctcccgaaa ctggggatca aaacgacgat taaaagcaat ctgcagtttg actcctttct
     1981 ctttaaccgc ccttagcacc gcaagggaag ctttctcatc ttctctcatg ctgagaggtt
     2041 tttcgcagaa aatatgttta cccgcctgga ctgctgccgt aacgatctct tcgtgcatat
     2101 ctgtcgaggt gaaaataaac accgcctcaa tatcatcacg ctgtaacatt tcctgataat
     2161 ctttatatcc tgaggttata tttaccgaac tcagccagtc gctcatttcc tcaataaaac
     2221 tgtcagcagc acagacaata tctaattgag gaagtaaata catattctca acatgcattt
     2281 tccctacccg gccaagacca ataatcccac atcgaagctt ttgcatttat acctcgtcat
     2341 atcatgatat agtaaatata aaacgacaaa agaaaatccc ctttatccaa cacggtacga
     2401 aagactttat tttgtagttt caacgaacaa atacttttac cgtaaaaaac gtcaggttgc
     2461 agaaaggcgc cctcctgcaa ccttaaaaac tgaattaatt agtactgtcg cgcttgtcca
     2521 atatgctcat caagcatacg agctactgcc tggatgcgtt cgctacgcga cacgcgagcg
     2581 ccaccaacac gccaccagct cagatattta tgcgccattg ttttaggcag tactttaatg
     2641 tctatcaacg tactcaccgt ctgtttacgt gcatcctcca gagcttcaag cagttgtcca
     2701 atgctggtga cgcgatagct tttacagcca tatccttcgg caatccgggc aaaatccaca
     2761 ggaatgagtc ccccctcctg acgaccgtta tcctgtttat ggaaacgaaa ctcggtaaag
     2821 aaactgtcca taccgtgttc catttgaaga ttatttatac aaccattagt catgttatcc
     2881 agcaaaataa cattaatttt cttccccatc tggaccgaag ttaccagctc agaatgcagc
     2941 atcataaatg agccgtcgcc aacgaagcta tatacctccc gctccggttg cgccaacttc
     3001 gcccccaatg cggcattgac ctcatagccc atacaggaat agccatattc tacgtggtaa
     3061 tcattatcgc cacggctttg ccagatgcgc tgcagatcgc ctggcagact tcccgccgca
     3121 gcaaccacca gcgcgttttc tggcagcatt cgatttagta ctccaagaac gcgcgtctgt
     3181 gtcagccatg aatgagtaat ttcattgaac tcttcaaaca ctttttgagt atccatatga
     3241 tccacaattt ccggtttgaa atccttaccg ctgtattccg ctgcatagac gcgatccact
     3301 tccgccagat attgagaacg aacacgatgg atcgttttcc cccattgtga tatgtaacct
     3361 tccggagcca gtcgttcgca aagctgcgtc agggcaacac gggcatcagc ctgcaattgc
     3421 actccatcca gtttgaagac gtcaaaacgg ctgacgttta tattcagata gcgcacatca
     3481 ggattctgga aaatccattt agaagaggtt gtgaaatcgg tataacgcgt acccacacca
     3541 ataatcagat cggcctcctg agccagcaaa ttcgccgcca gcgatccggt ttcaccaatc
     3601 ccaccaacat tcagcggatg cgcagaactg agcgcacctt taccagcctg cgtttcagca
     3661 atcggcagat gatattgttc ggcaaatcgc agcagttcct gttcagactg cgaatacttc
     3721 acacccccac cgcaaataat aattggtctc tggtggcgaa gaataagagc aactgcatca
     3781 ttcagcatta gctctgttgg cacgtggcgc tcaagacgat agacccgacg tgcaaaaaaa
     3841 tagtctggat aatcccaggc ttctccctga acatcctgcg gcaaacaaag cgtcacagct
     3901 cccgtatcag ccggatcggt aagcacccgc atcgcgctaa tgcaggcgct cattagctgt
     3961 tccggacgga taatgcgatc ccaatagcga ctcactgcac gaaaagcatc attggtgctc
     4021 agcgtcaggt catgctcctg ttcaatctgc tgcaacacag gatcgggttg acgcgatgca
     4081 tacacatctc ctggtagcag aagcaacggg atacggttcg ccgtcgcagt cgccgccgcc
     4141 gtgatcatat tagcggcacc aggccctacg gaggaggtgc aggcataaat ctgctgacga
     4201 cgcatctgac gcgcaaatcc tgttgctata tgtgccatac cctgttcatt gcgtccttga
     4261 tgaacgctaa gttgaccaca ctcttcttcc agagcctgcc ctaatcccac gacattccca
     4321 tggccaaaaa ttgcgaaaat accttttaca aacttgattt cgcgaccgtc cacttcaatg
     4381 tactgattat caagaaagcg caccaaagcc tgcgccatag tcaacctgat tgttttcatg
     4441 cactccacct atcttatcaa ccgtttgact gtcgttacct acgtacgcac tgctacagcc
     4501 ccgggcataa aacactcacc acaagaaaaa aattccgcaa acgcaattat atgaaacata
     4561 tttttcattt gtgggaagaa gatcaaaaaa cgattaaaaa tttcgagaat agctaaaata
     4621 cgaa
//
LOCUS       DAZKFU020000308         4618 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZKFU020000308 DAZKFU020000000
VERSION     DAZKFU020000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.310"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..497
                     /locus_tag="ACHOQQ_RS22010"
                     /old_locus_tag="pgaptmp_004402"
     CDS             <1..497
                     /locus_tag="ACHOQQ_RS22010"
                     /old_locus_tag="pgaptmp_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGU4472563.1"
                     /translation="EDRTDEGFDVECGFAAPWTRGNNCRHDEKTNNETDKTPPPEGRK
                     EQKLIVPEGDPGEWLRSLTPVQRKQLTQQLKNDQKSTKTANSGGFPAPEGVVYAGAAL
                     AKPDMTTRLIASTLSTTGEELHSSAALSVYRGARVRLPDGRIVHWDESTGSLKEVAQL
                     KGDE"
     gene            494..895
                     /locus_tag="ACHOQQ_RS22015"
                     /old_locus_tag="pgaptmp_004403"
     CDS             494..895
                     /locus_tag="ACHOQQ_RS22015"
                     /old_locus_tag="pgaptmp_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472564.1"
                     /translation="MTRFNCTLQQREFSVVMTKRLPALTLRGLIRRPQPGDQLEITIA
                     RPDRSTESMTADILNARPWGDSGDFIAALRLRNTGAPEPLTPAGRIMKAYHEKDIRKH
                     NEDLARAMKRINRKAAIASYVHSAARKQHRK"
     gene            915..1109
                     /locus_tag="ACHOQQ_RS22020"
                     /old_locus_tag="pgaptmp_004404"
     CDS             915..1109
                     /locus_tag="ACHOQQ_RS22020"
                     /old_locus_tag="pgaptmp_004404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472565.1"
                     /translation="MSTRQQHTINSTRAISDRDLNSMIESAQQLLSRQNPVVEETWWE
                     NLLAALTELRERRNNEGATK"
     gene            1106..1453
                     /locus_tag="ACHOQQ_RS22025"
                     /old_locus_tag="pgaptmp_004405"
     CDS             1106..1453
                     /locus_tag="ACHOQQ_RS22025"
                     /old_locus_tag="pgaptmp_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGU4472566.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEEERLELAQRAEWQQEKSVIEVCKRSEVMKRILFI
                     YGAGGDLPVISVQ"
     gene            1477..1794
                     /locus_tag="ACHOQQ_RS22030"
                     /old_locus_tag="pgaptmp_004406"
     CDS             1477..1794
                     /locus_tag="ACHOQQ_RS22030"
                     /old_locus_tag="pgaptmp_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4472567.1"
                     /translation="MTTQIATRGRLAEDPTTRKSSRGTDVTAARLAISLPGHGAEQST
                     TMWLYLIAFGKQHEELAKHKKGDLVQVHGNLQVNRYQDKYGQMKEGWQLLAQSIISSR
                     TAI"
     gene            2049..2423
                     /locus_tag="ACHOQQ_RS22035"
                     /old_locus_tag="pgaptmp_004407"
     CDS             2049..2423
                     /locus_tag="ACHOQQ_RS22035"
                     /old_locus_tag="pgaptmp_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015834182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HGU4472568.1"
                     /translation="MKNNDHGLNTLLDMHGYQHHMDNGYWWKIEAYRVSPSQFRPHGI
                     RYNLTLHDPYNSRVFGMDNAHGIKPPKKGKFTGRITVYDHIHRTSSDKGYPYEFTSAE
                     QLLLDFFNHVDRVIAEQQRGKK"
     gene            2420..2797
                     /locus_tag="ACHOQQ_RS22040"
                     /old_locus_tag="pgaptmp_004408"
     CDS             2420..2797
                     /locus_tag="ACHOQQ_RS22040"
                     /old_locus_tag="pgaptmp_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4472569.1"
                     /translation="MKARIGIIPEKVLRQRMLDIAKGKLKPEPDEPRVWFSSLNALGQ
                     ALSNENIALLRIMDAEKPQTMTELASLSGRQLSNLSATLKMLSRYGFVSLEKQGNCVQ
                     PKALFTDFEIIIDPSVGEAHRAA"
     gene            3333..3836
                     /locus_tag="ACHOQQ_RS22045"
                     /old_locus_tag="pgaptmp_004409"
     CDS             3333..3836
                     /locus_tag="ACHOQQ_RS22045"
                     /old_locus_tag="pgaptmp_004409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472570.1"
                     /translation="MSHFRDLYLANKKSRDARREVTGILRNQAEKLVAFYEQWLELPS
                     ETWEDKDGEHHRYVDTGFPCRNDRDFRPFPVNMIATDTDNIFRMAVRTWVDSHDEGES
                     VNIVLAMELHSVGKDGVSLRVRVEDDTSIRVVINGDGDREWLPIVNSMKMHIISKLQR
                     RTPSAFR"
     gene            complement(3902..>4618)
                     /locus_tag="ACHOQQ_RS22050"
                     /old_locus_tag="pgaptmp_004410"
     CDS             complement(3902..>4618)
                     /locus_tag="ACHOQQ_RS22050"
                     /old_locus_tag="pgaptmp_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4472571.1"
                     /translation="FGNAYLEVRRNRLGEPLLLRPALAKYTRRGSDLDTYWYLNDDGT
                     EFSFRKGTVCHVLNPDINQEIYGMPEYIGGLLSVSLSNSADTFRKLYYDNGSHAGCII
                     YVGTPQANAESVEAIKKTLTDSRGRGAFKNLFLHAPGGGKDGVQILPFQQITAKDEFL
                     NIKGSARDDILAAHRVPPQLMGAMPDGNAAFGDVEKAARVFFINELQPVMEAMKHVNE
                     WLGVEVMRFNPYALLLDNAS"
BASE COUNT         1397 a         1106 c         1215 g          900 t
ORIGIN      
        1 acgaagaccg cacggacgag ggttttgacg ttgagtgcgg ctttgccgca ccttggactc
       61 gtggcaataa ctgtcgccat gatgaaaaaa caaacaacga aacggacaaa acaccaccac
      121 cggaagggag gaaagaacaa aaactaatcg taccggaagg tgatccgggt gagtggttaa
      181 ggtcactgac tccagtacag agaaaacagc tcacccaaca gctgaaaaat gaccagaaat
      241 ccacaaaaac cgcaaacagc ggcgggttcc ccgcacctga aggcgtggta tatgccggtg
      301 cggcactcgc aaaaccggat atgaccacca ggttgatcgc cagtactctg agtacaacgg
      361 gcgaggagtt acacagtagt gcggcgcttt ccgtgtatcg cggggcgcgc gttcgcctgc
      421 ctgacgggcg gattgttcac tgggatgaaa gcaccggaag cctgaaggaa gtagcacagc
      481 tgaaggggga tgaatgacgc gattcaactg cacgcttcag caacgagagt tcagcgtcgt
      541 catgaccaag agattaccgg cgctgacgct gagaggactg ataagaagac cgcagccagg
      601 tgaccagctg gaaatcacca tagcccgtcc agacaggagt accgaaagta tgactgccga
      661 catcctgaac gccagaccat ggggagacag cggcgacttt atcgcagccc tgagactgag
      721 gaacacggga gcaccagaac cactgacgcc agccgggagg atcatgaagg catatcacga
      781 aaaggatatc aggaaacaca acgaagattt agccagggcg atgaagcgca ttaaccgcaa
      841 ggcggccata gcaagttatg tacattcagc cgccagaaag cagcaccgca agtaacagga
      901 gaaccacaga catcatgagc accagacagc aacacaccat taacagcacc agggcgatca
      961 gcgacaggga cctgaacagc atgattgagt cggcgcaaca gctgctttca cgccaaaatc
     1021 cggtcgtgga agaaacatgg tgggaaaatt tactggcagc actgaccgaa ctccgggagc
     1081 gccggaacaa cgaaggagcc accaaatgaa gaccccagga atgaccggaa aggaaatcat
     1141 tttacgctac ctggaaacgc acgcacgctt cagcgcaccg gaactggtag aaaaactggg
     1201 cgtgaaaaga accgtcatca atcaggccgc cggaaaaatg gtacgggaag gcctgctggt
     1261 tgtggacggg tacacggaca agataacgca ttaccggaag ccaacggaag aggaacgact
     1321 ggagctggcg caacgggctg aatggcaaca ggaaaaatca gtcatcgaag tctgcaagcg
     1381 cagcgaagtc atgaagcgaa ttctgtttat ctacggcgct ggcggagatc tgccagtcat
     1441 atcagtccaa taactaaacc accaacggag acaaatatga caacgcagat tgcaacacgg
     1501 ggacgactgg cggaagaccc gacgacaaga aaatcatcca gaggcaccga tgtaacagct
     1561 gcacgactgg cgatatcact gcccggccat ggcgcagaac agtcaaccac aatgtggctt
     1621 taccttatcg cattcgggaa gcagcacgaa gaactggcaa aacacaaaaa aggcgacctg
     1681 gtacaggtgc acgggaactt gcaggtaaac agatatcagg acaaatacgg acagatgaaa
     1741 gaaggctggc agttgctggc acaaagcatt atcagctcaa gaaccgcaat atgatcaata
     1801 tgtgagtaat gatgtgagac gccgggctgg cacaaacaaa aaggcggaga aacatactcc
     1861 gcctgacaca gttttacgta ttcagaagcc gtcaaattcg tcagacactt ttttcatatt
     1921 aaccactacg aattcaaata tgtacataag ttaacgtcga taaccacaag aacagcacaa
     1981 taacccacct tgataaagaa aaaaacgatg ttatgatttg gtcataagta aggtgatacg
     2041 aatcagttat gaaaaacaat gatcatgggc tgaacaccct tctggatatg catggatacc
     2101 agcaccacat ggataatggt tactggtgga agattgaagc atacagggtt agcccaagcc
     2161 agttcagacc acatggaata cgctacaacc tgacattgca cgatccttat aactcccggg
     2221 tttttggaat ggataacgct cacggaataa agccaccaaa gaaagggaaa tttaccgggc
     2281 gcattaccgt ttatgaccat attcacagaa catcgtccga taaaggctat ccatatgaat
     2341 tcacttctgc ggaacagctt ttactggatt tctttaacca tgtagacaga gtgattgctg
     2401 aacagcaaag ggggaagaaa tgaaagcccg tatcggcatt atcccggaga aagtccttcg
     2461 ccagagaatg ctggatatag ccaaagggaa actaaaacca gaacctgacg aaccgcgagt
     2521 ctggttttca tctctgaacg ctctgggtca ggcgctgagc aacgaaaata tcgcgcttct
     2581 gcgtatcatg gatgcggaaa aaccgcagac aatgacagaa cttgccagtc tttcaggaag
     2641 gcagttaagt aatttgtcag caacgttaaa aatgctgagt cgatacggct ttgtcagcct
     2701 ggaaaaacaa ggtaactgcg tacagccgaa agcgttgttc acggattttg aaatcataat
     2761 agacccgtca gtaggtgaag ctcatcgggc ggcatgaata tcgcgggggc aatccccgct
     2821 catgcttttt ttagcctgcg attcccgcat aaacaccacc tggaaaaccg cacaaaagtg
     2881 cacgaaattg cacaattttt cttatgtgtt ttttaccctt tcagcccagt aacggcgctg
     2941 gctgaagcca ctttgcacgg tgcacgaaaa acgaggcgat cgctgcgcgc aggcgacgag
     3001 ggaagggcgc ccgaattgag ggatggaagg cgggttgctt tatttggggt cgtattgcgc
     3061 gtgatacgca cgcagtaaag tcagttttga aaaaccacat caaaacatgg tcagggaaac
     3121 aggccgtcag aatgcgtctg attgcgtctg gcgaggtcgg agatcgggag ggaaacggac
     3181 gtgacgaacg ggataaaaaa gagagacaaa aatcattgaa atcataaagt taatagaatt
     3241 taaagtatca gtcattcacg ggtgaaaact tgcgcacaat atgaggtgag cgcagaatac
     3301 aggctggcat aaacagacca aaggagagcg acatgagcca ttttcgcgat ttgtacctgg
     3361 caaacaaaaa gagccgggac gcgaggcggg aggttacagg gattctgcgc aatcaggctg
     3421 aaaagctggt tgctttttat gagcagtggc tggaattgcc ttcagagaca tgggaagaca
     3481 aagacggcga gcatcatcgt tacgttgata cggggtttcc gtgcagaaac gatcgggact
     3541 tcaggccatt tccggtaaat atgattgcaa ccgacacaga taacatattc cggatggctg
     3601 taagaacatg ggttgactca cacgatgaag gagagtcggt aaacatagtc ctggcaatgg
     3661 agttacattc cgtcggaaaa gacggtgtat ctcttcgggt tcgcgtggaa gatgatacct
     3721 ccattcgcgt ggtcatcaac ggagacggtg acagggaatg gctgccgata gtaaacagca
     3781 tgaagatgca tattatcagc aagctgcaaa gaagaacgcc atccgcattc cgttaagaga
     3841 gctggagagt cggggcataa attttttatg ccccggcgaa gcagcagaca agcgaagcgc
     3901 gtcagctggc gttatccaga agcagggcat aggggttaaa gcgcatcacc tcaacgccaa
     3961 gccattcatt gacatgcttc atggcctcca tgacaggttg caactcgttg atgaaaaaca
     4021 ctctcgccgc tttttcaacg tcaccaaacg ccgcattccc gtcaggcatt gcccccatca
     4081 gttgaggtgg tacacgatgg gcagcaagga tatcgtcgcg ggcgctcccc ttgatgttca
     4141 ggaattcatc ctttgcggtt atctgctgga acggcagtat ttgcacacca tccttgccac
     4201 cacccggcgc atgtagaaac aggtttttaa acgccccgcg accacgggaa tccgtaagcg
     4261 tttttttaat ggcctcgaca ctttccgcat tcgcctgagg tgtgcctaca tagataatac
     4321 acccggcatg cgagccgtta tcgtagtaga gtttgcggaa tgtatccgct gagtttgaca
     4381 ggctgacaga cagcaacccg ccgatatatt ccggcatccc gtagatctcc tgattaatat
     4441 caggattaag cacgtggcac acagtccctt tcctgaaact gaactccgta ccgtcgtcgt
     4501 tgagatacca gtaggtatcc aggtcacttc ccctgcgtgt atattttgcc agtgccgggc
     4561 gaagcaggag tggttccccc agcctgttac gccgcacttc aagataggca ttaccaaa
//
LOCUS       DAZKFU020000309         4601 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZKFU020000309 DAZKFU020000000
VERSION     DAZKFU020000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.311"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(30..350)
                     /gene="chbB"
                     /locus_tag="ACHOQQ_RS22055"
                     /old_locus_tag="pgaptmp_004411"
     CDS             complement(30..350)
                     /gene="chbB"
                     /locus_tag="ACHOQQ_RS22055"
                     /old_locus_tag="pgaptmp_004411"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGU4472572.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(649..987)
                     /gene="osmE"
                     /locus_tag="ACHOQQ_RS22060"
                     /old_locus_tag="pgaptmp_004412"
     CDS             complement(649..987)
                     /gene="osmE"
                     /locus_tag="ACHOQQ_RS22060"
                     /old_locus_tag="pgaptmp_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGU4472573.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1189..2016
                     /gene="nadE"
                     /locus_tag="ACHOQQ_RS22065"
                     /old_locus_tag="pgaptmp_004413"
     CDS             1189..2016
                     /gene="nadE"
                     /locus_tag="ACHOQQ_RS22065"
                     /old_locus_tag="pgaptmp_004413"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGU4472574.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2246..3133
                     /gene="cho"
                     /locus_tag="ACHOQQ_RS22070"
                     /old_locus_tag="pgaptmp_004414"
     CDS             2246..3133
                     /gene="cho"
                     /locus_tag="ACHOQQ_RS22070"
                     /old_locus_tag="pgaptmp_004414"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGU4472575.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSQAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3093..3668)
                     /gene="ves"
                     /locus_tag="ACHOQQ_RS22075"
                     /old_locus_tag="pgaptmp_004415"
     CDS             complement(3093..3668)
                     /gene="ves"
                     /locus_tag="ACHOQQ_RS22075"
                     /old_locus_tag="pgaptmp_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGU4472576.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3871..4356)
                     /gene="spy"
                     /locus_tag="ACHOQQ_RS22080"
                     /old_locus_tag="pgaptmp_004416"
     CDS             complement(3871..4356)
                     /gene="spy"
                     /locus_tag="ACHOQQ_RS22080"
                     /old_locus_tag="pgaptmp_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGU4472577.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1159 a         1111 c         1103 g         1228 t
ORIGIN      
        1 aactctttga cgggaaaatt taaaataaat taatttgctg cggctttttt aatcgctgca
       61 accgcagcct taagcacgcc taaaccatcg actttgccat aaagcagcga gtcaattact
      121 tcaaccggtt tgttgggtaa caaacgctgg atttcgggca acatataagc aatctgcggc
      181 cctaataaca cgacatcggc attctgacct ttttcaccag ccagtgtttc cggaaatgct
      241 tcaataatga ccggaacttc atatttttct gcctgtgcgc gcatttttga taccagtaaa
      301 gaggtagaca tgcccgcaga acaaaacaga taaatgtgtt tcttttccat aaaactgccc
      361 tcatcgacga ttatctgtca gccagacact ccgcaagcct taacctgctt ccatgctctg
      421 ggtaacttgc gaaaccaaca tgactttttt tgtggctgaa aggagtatac ggtatagacc
      481 tgagcaatag tatttctttg accttataaa ttgtctctcc ttgacctgcg gttatgaccc
      541 tcttcacatt tcgggcaaat aattcgcgaa gataattaag aactcagacc aaaaaaaacc
      601 ggcgcaatgg ccggtttccg ttgttactca agctttcaga cgaagtgatt acttcgcagc
      661 ctgtggatca gtgtcgtatt cagcacaggt ctgataaccg gagttgatga catgtccggt
      721 atcatctaac gcgacaaagt aggtttctgc tttaccatca cgttgaccca ggatgtaggt
      781 ctggcaagta ccacgagcat ggatcatgct cacttcagac gaaggtttac ccgcaatttg
      841 tgcaacctgc gcccggctca tgcctttttt gacgtctttc accacaggct gtacaaactg
      901 gtctttggta cgatcataag ccgtacaacc cgccagcatg gttaataccg ccgctgcact
      961 cagaattcct gccatattct tgttcatatt ccgtcctctt gtttatcagc gtgttagata
     1021 agcctggaat acattgggcg ctttttcaag cccgtgaacg aaacggctcc gctttcagag
     1081 gattcctgta tgacgtttta accaccattc agcccgctgt cgcttgtcgt ttcagtagca
     1141 acgggttagc tttaaggaag ttttgtcttt tctgtctgga ggggttcaat gacattgcaa
     1201 caacaaataa taaaggcgct gggcgcaaaa ccgcagatta atgccgaaga ggaaattcgt
     1261 cgtagtgtcg attttctgaa aagctacctg cgaacttatc cgttcattaa atcactggtg
     1321 ctcgggatca gcggcggtca ggactccacg cttgccggta agctgtgcca gatggcgatt
     1381 aatgagctgc gccaggaaac cggcaacgaa tcactgcaat ttattgccgt acgcctgccc
     1441 tatggtgttc aggccgacga acaagattgc caggatgcca ttgcctttat tcaaccggat
     1501 cgcgtattaa ccgttaatat caagggcgcg gtattggcta gcgagcaggc attgcgggaa
     1561 gcaggcattg aactgagcga ttttgtccgt ggcaatgaaa aagcgcgtga gcggatgaaa
     1621 gcacaatata gcattgcggg tatgaccagc ggtgtcgtgg tgggcaccga tcatgcagca
     1681 gaagccatta ccggattctt cactaaatat ggtgacggcg gtacagacat taatccgctg
     1741 tatcgtctca acaaacgtca gggtaaacag ttactggcgg cattaggttg cccggaacac
     1801 ctttataaga aagcgccaac ggccgatctg gaagatgatc gcccttctct gccagatgaa
     1861 gtggcactcg gcgtgaccta tgacaatatc gacgactatc tggaagggaa aaacgtacct
     1921 caacaggtcg ccagaacaat agagaactgg tatctgaaaa ccgaacataa acgccgtccg
     1981 ccaattaccg ttttcgatga tttctggaaa aagtaataat ttgcacctat tggattgtgc
     2041 gaaaaagagt aatttgttca cgccggatgc ggcgtgaacg ccttattcga cctacaaaac
     2101 tatgcaaatt caatacattg caggagtcga atgggcctga caggcgtagc acgtcagacg
     2161 gtgtaacctt tgtcatcaac ccgcctcttt tttaatcgct tcacgcctgt tacactggat
     2221 agataaccag ccttcggagt caacagtggt acggcgttta acttctccgc ggctcgaatt
     2281 tgaagctgcg gcaatttatg aatatcccga acatttacgt tcattcctta atgacttacc
     2341 cacccgacca ggggtgtatc tgtttcatgg cgaaagtgac accatgccgc tctatatcgg
     2401 caaaagcgtt aatatccgca gtcgggtgct ttctcattta cgcaccccgg atgaagccgc
     2461 catgctacgg caatcccggc gaattagctg gatctgcacc gcaggcgaaa tcggcgctct
     2521 gctccttgaa gcgcgattaa tcaaagaaca acagccgctg tttaataaac ggttgcgccg
     2581 caatcgccag ctctgtgccc tgcaattaaa tgaaaagcgc gtcgatgtgg tgtatgccaa
     2641 agaggtggat ttttcacaag cccccaacct gtttggcctg tttgccaata ggcgcgcagc
     2701 tttgcaagca ttgcagacca tcgctgatga acaaaaactt tgttatggcc tgctgggact
     2761 ggaaccgtta agtcgcggtc gtgcatgttt tcgttcagcg ctaaaacgtt gcgccggagc
     2821 atgctgcggt aaagagagcc atgaggaaca tgcgctacgc ttgcgccagt ctctggagcg
     2881 tttgcgggtg gtgtgttggc cttggcaagg tgcggtggca ctgaaagaac agcacccgga
     2941 aatgactcaa tatcatatta ttcaaaactg gctgtggctg ggggcggtta attcgcttga
     3001 agaagcgaca acgttaattc ggacacccgc cgggtttgat cacgacggtt ataaaattct
     3061 ttgtaagccg ctgctttccg gtaactatga aattactgaa cttgatccgg cgaatgacca
     3121 gcgagccagt tgatttcact gaacaacagt ttaccttgcg gttgtaaaag ccgcagggtg
     3181 tgcctgccat cgaaccagca cgcgccctgg tcggtagtga gcagtttgtc gcccagttgc
     3241 cacgccccgt taatcacaaa caccaccccg ccgcgtgagc caaaggtagt aaacgtacgt
     3301 tcggcaattc tgactttcgc cttacagaca tccaggcgtg tcatgatgtt gaaatccatc
     3361 gacatctgcc cttccgtcag tttcgctttt accacctggt ccgccgcaaa ggcaaaaggt
     3421 tgtaacggtt ttaaagtatg gttaaagcgg tctgtgcttt caagaaacat ctcgccgcct
     3481 tccagcaacg tcactatcct ttccataccg ggaaacaaag aaaactcccc attagccgcg
     3541 atggatgcaa tgctggcacg ccagtaaaaa tcacgttttg ccggtgggaa agtgcaaatt
     3601 tcgcgcgttt caccggcagc gtttcgccac agattcaccg acattttacg catatcaaag
     3661 tattccatac tcgctcccgg cccgttcttt tcattgttat cgttcaggta accgtggatg
     3721 gttatcggca actcgggtga tttacgttag tggtgatcag gcttttttta caactgtttg
     3781 agaagagaaa agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga
     3841 ccggcggtct taagtttttt ggctgaaaga ttattcagca gttgcaggca ttttaccttt
     3901 tgccgctgga cgttctgtca gacgcttctc aaaattagca ttaaattgct ttttctgttc
     3961 cggcgtcagg atgttgtaaa ttttgttctg ggtttccatg tgcgccagca tgttagcttt
     4021 gcgctgttct tccatttttg cgatctgcgc ttcagctttt gctttatcga aggtatcgct
     4081 ggcaatgatg tcatgcattg cgcggcgttc ttccagcggc ggacgtttca tctggtcacg
     4141 ctggcctttc atgatttcgc ggatctgctg tttctgcgcg tcggtcaggt tcaggtcttt
     4201 gaacatcatg tcctgatgcg gaccgaactt gcctttgtgg tgcatcatcg gcttcgcgtc
     4261 agccggtgct gcggtagtgg tgtctgcggc atgggccagg ttagccgcgc caagagccag
     4321 ggtagaggca acaaacagtg cagttaattt acgcatattc tatatccttc ctttcagtta
     4381 tttattacgg ctttcttaag tagcgtgccg tgttgacgag attaacttta ctgactttag
     4441 cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtgta aaaaacactt ttgcgccaat
     4501 tatggagaaa aaaagaaaat ttgatggaga gtgatgagcg aatattacaa cacgatgatt
     4561 ttgcagagat tatgaagaac tataccggat gactggtgat a
//
LOCUS       DAZKFU020000310         4506 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZKFU020000310 DAZKFU020000000
VERSION     DAZKFU020000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.312"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..492)
                     /locus_tag="ACHOQQ_RS22085"
                     /old_locus_tag="pgaptmp_004417"
     CDS             complement(34..492)
                     /locus_tag="ACHOQQ_RS22085"
                     /old_locus_tag="pgaptmp_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGU4472578.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            612..1421
                     /gene="proC"
                     /locus_tag="ACHOQQ_RS22090"
                     /old_locus_tag="pgaptmp_004418"
     CDS             612..1421
                     /gene="proC"
                     /locus_tag="ACHOQQ_RS22090"
                     /old_locus_tag="pgaptmp_004418"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGU4472579.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRSQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1438..2553)
                     /gene="adrA"
                     /locus_tag="ACHOQQ_RS22095"
                     /old_locus_tag="pgaptmp_004419"
                     /gene_synonym="dgcC"
     CDS             complement(1438..2553)
                     /gene="adrA"
                     /locus_tag="ACHOQQ_RS22095"
                     /old_locus_tag="pgaptmp_004419"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGU4472580.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2655..2975)
                     /gene="psiF"
                     /locus_tag="ACHOQQ_RS22100"
                     /old_locus_tag="pgaptmp_004420"
     CDS             complement(2655..2975)
                     /gene="psiF"
                     /locus_tag="ACHOQQ_RS22100"
                     /old_locus_tag="pgaptmp_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGU4472581.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3094..>4506)
                     /gene="phoA"
                     /locus_tag="ACHOQQ_RS22105"
                     /old_locus_tag="pgaptmp_004421"
     CDS             complement(3094..>4506)
                     /gene="phoA"
                     /locus_tag="ACHOQQ_RS22105"
                     /old_locus_tag="pgaptmp_004421"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGU4472582.1"
                     /translation="KQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGAR
                     RLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFNGIDALPLT
                     GQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAA
                     GLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLN
                     ARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPL
                     LGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKN
                     EKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADHA
                     HASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAANV
                     VGLTDQTDLFYTMKAALGLK"
BASE COUNT         1036 a         1227 c         1147 g         1096 t
ORIGIN      
        1 attcaagaat gaagtgtaaa taataaatta catttagcca cgactacgtt gcacttccag
       61 ccaccacttc tccagctccg cggcaaatgc ctggcggtca cgttgtgaaa ggctatctgg
      121 tccgccggtc tggatcccac tggcacgtaa ggtatccata aaatcgcgca tcgtcaggcg
      181 ctcacgaatg gtcgctggcg tgtaacgttc accgcgcgga ttaagcgccg cagcgccttt
      241 ctcgatggct tcagcagcca aaggtatatc tgcggtgatc accaaatcgc ccgcttcaca
      301 ctgccggaca atttcgttat cggcaacgtc gaaacctgcc gcgacgcgca gcgtacgaat
      361 aaatcgcgat ggcggcacgc gtaaactctg gtttgctacc agtaccagcg gcatctgcat
      421 acgttccgcc gcgcgataca aaatctcttt aattacattg ggacacgcgt cggcatccac
      481 ccaaattgtc ataaagtcat cctttgttgg gtaatcctct attgtgtcgc gcttttgcct
      541 tccggcatag ttctgtttat gcttctgcca gcgattatca aaacaatgaa tttcacggca
      601 ggagtgaggc aatggaaaag aaaatcggtt ttattggctg cggcaatatg ggaaaagcca
      661 ttctcggcgg tctgattgcc agcggtcagg tgcttccagg gcaaatctgg gtatataccc
      721 cctccccgga taaagtcgcc gccctgcatg accagttcgg catcaacgcc gcagaatcgg
      781 cgcaagaagt ggcgcaaatc gccgacatca tttttgctgc cgttaaacct ggcatcatga
      841 ttaaagtgct tagcgaaatc acctccagcc tgaataaaga ctctctggtc gtttctattg
      901 ctgcaggtgt cacgctcgac cagcttgccc gcgcgctggg ccatgatcgg aaaattatcc
      961 gcgccatgcc gaacactccc gcgctggtta atgccgggat gacctcggta acgccaaacg
     1021 cgctggtaac cccagaagat accgctgatg tgctgaatat tttccgctgc tttggcgaag
     1081 cggaagtaat tgctgagccg atgatccacc cggtggtcgg tgtgagcggt tcttcgccag
     1141 cctacgtatt tatgtttatc gaagcgatgg ccgacgccgc cgtgctgggc gggatgccac
     1201 gctcccaggc gtataaattt gccgctcagg cggtaatggg ttccgcaaaa atggtgctgg
     1261 aaacgggaga acatccgggg gcactgaaag atatggtctg ctcaccggga ggcaccacca
     1321 ttgaagcggt acgcgtactg gaagagaaag gcttccgtgc tgcagtgatc gaagcgatga
     1381 cgaagtgtat ggaaaaatca gaaaaactca gcaaatcctg atgactttcg ccggacgtca
     1441 ggccgccact tcggtgcggt tacgtccggc tttctttgct ttgtagagcg ccaaatctgc
     1501 cgatttcaac cactcacgat agtgactcat ttgtgggttc agcggcgcaa cccccacact
     1561 aatccgtaaa gttacctgtg gcgtattcgg caaacgtaat gtatttagcc cttcatgcac
     1621 ccgtaacatg gcggtaatgg cgctctcagc tggcgtaccg gacatgatta ctgcaaactc
     1681 atcgccgcca aaccgaccaa tcacatcgct accacgcagg gtaatttgta actgtcgggt
     1741 aagcgccaca atcgcttcat cgcccacatc atggccccag gtatcgttga tgctcttgaa
     1801 atggtcgata tcgataatca gtaacgttgc atcgcgatta tgccgccgac agttatcaaa
     1861 ttcattgcgt aacatagttt cccaatgacg tcggttatac acgcccgtca tgccgtcgcg
     1921 ggtactcatg acctgcaacc tgcgtttatg ttccgccagt ttagtcgccg tctggtagct
     1981 gacccagcca aacagcagag gataaatgac aataatggga agggagagcc accattccag
     2041 cggcgcacta ttgaacgaca cggtgatgcc cgtcagctcg agggtgacaa ggcaggaaac
     2101 caccatcaac accagacccg cgacaaacag acgggggccg cctgccccca tcaaattcag
     2161 acacataatc atcaacatcg cggtggaagg cagcacgttt acgcccatta cgcctaccca
     2221 cattcccgct aatactgcat cggtttttaa gttgtaaatt tcccggctaa gcggatcgac
     2281 ggccctgctc gctatctgcc aggctaaatg cggccagacg aacgcccagc cgaccaacac
     2341 cagccaccac cagcccggcg gcgggtgtga aaccagcgtt gaagcaatcg gtaagaacat
     2401 gccagccagg ccaaccgcac ggggtagtct gacgcgacgg gcgaagcgca gcccggaccg
     2461 ctgatgttcg ttttgaggag ttaaaggagg ttcctccccg tgcgccgccg cttttttgaa
     2521 aaagttttca tcattcatta tttttgggaa cattcagaaa caattttccc aaattataga
     2581 gacggggcta aaggcagaag tatgaaattt cgggtgagcc attttagtag ctcacccgtt
     2641 agcactatca ggtatcaggc ggctttcttg aggcaggcgc tcataaactt attacgatca
     2701 tcacctttca gtgattgttg cgttgcttga ttattacatt cgcgcatctt ttgctgctgt
     2761 ggtgtcaaac ttttttcacc aggcgcagac ttgctgttct tcaggcaatc actcatgtag
     2821 gtcttacgag catccccttt caacgcctgt gccgtcgcct gctgattaca ggaggtcata
     2881 cgctgttgtt gtggggttaa ggttctctcg gcagcgccga cggtggttaa aaaaaccaga
     2941 ccgaaaagca aggtaaccag taatgtaatt ttcatagcac catccctctt catgttttaa
     3001 ccatgagcgt atgcgcccgt gatctgccat taagtctggt tgctaacagc aaaaaaacca
     3061 cccggcagcg aaaattcact gccgggagcg gttttatttc agccccagag cggctttcat
     3121 ggtgtagaag agatcggtct ggtcggtcag tccaacgaca ttggcggcat gcgggccata
     3181 cgccgcaata cgcaactgac tgccggtatg ttcttgtgaa tcctcttcgg agttcccgta
     3241 actcatcacc atcactgcgc catctttggt atttagcgcc tgggtgaggc ccggagcttt
     3301 ggtatccggc gcaacaatct ggctggcgtg ggcgtgatca gcggtgacta tgaccagcgt
     3361 gttaccatcc tttttggcga attccagcgc ccgttgtacg gcttcatcga gatcgaccgt
     3421 ctcgccaatt tgcccacaag gattcgcagc atgatcctgt ttatcgattg acgcaccttc
     3481 aacttgcagg aaaaagcctt tctcattttt actcaataat tcaatggctt tgtcggtcat
     3541 ctgtgccagg gtcggtacac tgtcattacg ttgcggatta ggcgtacagg tgactgcggg
     3601 cttatcgata ttaccgtggt acgttgcttt cggtcctagc cagcgcactg gcatattgcc
     3661 gtcagcaaac aggccaagca ggggtttttg ctgattcgct tccgtcaccg aattcagtga
     3721 ggcagcatcg ctcaccaact gataaccacg cgcctgtgcc tgttcacgca gcgtttttcc
     3781 ctgccattca ccagcggttg ccgtttcagc aaaggttttt gcgccgccgc caagcgtaac
     3841 gtcggcacga gcgttaagca gctgttcggt aatcgatcct tttccgcctt tttccagagc
     3901 gttacccgga catttttcac tggtcgcgct cggaccgtag catttgcgcg aagtcacatg
     3961 tgccaccagc gcagcgggcg tggcatcctg caactctgcg gtagaaacgt taccggtcgc
     4021 cagacctgcg gcttttgcca tttccagaat cgttgggtga tctttttcgt gaatatcgac
     4081 gcccagcgcg ccgttatagg ttttgacacc ggttgaccag gcggttgctg atgcagccga
     4141 gtcggtgacg tagtccggtt tgccggtttt tttattcagc gcatagtgag tgtattgccc
     4201 ggtaagcggt aaggcatcga taccattaaa aaagccgccc gcaccttcgg cataattacg
     4261 tgcggcagta atttccgaat cccccatccc atcgccaatc agcaaaataa tattttttgc
     4321 aggtttatcg ctaagagaat cacgcagagc ggcggtctga tcagcagtta agcggcgagc
     4381 accgccgggt gcagtaatat cgccctgagc agcccggttt tccagaacag gcatttctgg
     4441 tgtccgggct tttgtcacag gggtaaacag taacggtaag agtgccagtg caatagtgct
     4501 ttgttt
//
LOCUS       DAZKFU020000311         4493 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZKFU020000311 DAZKFU020000000
VERSION     DAZKFU020000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.313"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..3777
                     /locus_tag="ACHOQQ_RS22110"
                     /old_locus_tag="pgaptmp_004422"
     CDS             <1..3777
                     /locus_tag="ACHOQQ_RS22110"
                     /old_locus_tag="pgaptmp_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HGU4472583.1"
                     /translation="KSHKFWAVEQQGNELHISWGKVGTQGQSQVKNFADVAAAEKAKL
                     KLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAVIRLTDDINR
                     FAFPHRSRPREINYLHKDGQIWQLIANNTKAYDPANNYRSYPENWQQAFAELQMRVQG
                     NQQTGSVQSDAALLWSFWNSYSADELIDDLVIRCGLESAVEIALLALQLRYKPVKADV
                     TTIIPPDLKAESLPNWHQRLCHHLSLASEDEWQRCVDKVLAAIPSLSPARQPFAALLI
                     PERPDIANAMALHYADQNVPAMTWLSMMVSDDVALATLEKYGFPPLYNDFRKYLATLL
                     ANNGMRGVSRILLKLPVDYPVKYTDLFTHIHANAEDLVKWLWKTNHPDAIQILILGVN
                     GKKKHLEYLSKACQKHPAAAIAAYATLLAIHEDKEWRKALVKLITATPELVCDVIPWV
                     NAKAAGILSECRPQPVAEECEYATVDMLPELLVSPPWMTKEKKKNTPVFDLPVLPVPS
                     VSDVTPEITKKLTRTYLVTHFQQIAQQQATKQTLFTDLPPIKKASWEKHLIPLTPEQQ
                     ILWHLGFEKWRESGEKIYEKIPAPQSAVDALLRFDFPALNAEFVQYHNNAYKSWNLIA
                     LCYLPGQQAISFLNQIVKEDNYSGEGNILAIFGSAAIPAFMACLQGDPRRLCFFPFFL
                     GVSELALPMAQQLQKKMSYEDARNWLTDYPRHAAAGLLPVALGKKGKDRDCARQALRL
                     LVNLNQRETIEEIAQGYNQPDVLAALATLFDSDPLEEYPAKIAPLSGFYQFTLWRRPR
                     LKSNNLPLSDDAMRHLGTMLSFPRDITAYAGLDIIREIFTRESLAEFGWDLYTAWTEA
                     GAPAKENWAFTSLGILGNDDTARKLTPLIRAWPGESQHKRAVSGLDVLADIGSDVALM
                     LLNGIARKIKFKALQEHAREKINIVAENRGLTMAELEDRLAPDLGLDSSGSLILDFGP
                     RKFTVGFDETLKPVVCDANGKVLKDLPKPNQSDDKTLATDAVNLFKQLKKDVRAIASQ
                     QITRLEQAMCQRRRWTAEQFRLFLVEHPLVRHLTRRLLWGVYNDENALITCFRVAEDS
                     TYSDAQDELFTLPAGNIGIPHVLEISPESAAAFRQIYADYELLPPFQQLERGSYHLAD
                     NERNTHELTRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKTTPHGDLELETEPFS
                     LIYGETGYGDQLPVESVKITSPDDRYGKQSSLTFSMLDDITASELINDIESLFD"
     gene            3790..>4493
                     /locus_tag="ACHOQQ_RS22115"
                     /old_locus_tag="pgaptmp_004423"
     CDS             3790..>4493
                     /locus_tag="ACHOQQ_RS22115"
                     /old_locus_tag="pgaptmp_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HGU4472584.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQENELHITWGKVGSNGQSQVKSFAD
                     ATAAEKAELKLIAEKVKKGYVEQVDANAHSHDASAKKVLVTEEKASINRQAATNGLPW
                     LADNDPIILPPNIARHALSHRLWPGEPVKKPTKPEKQNLISRLANRTYHYYKNLTFDY
                     SSCSDEWADAISQAISLIKAQKTEIPARTMAVLTFLEICFCQGWNNETSSELLDEIVS
                     TFGIEYATETVIFWWQ"
BASE COUNT         1269 a         1112 c         1048 g         1064 t
ORIGIN      
        1 aaatcacata aattctgggc ggtggagcag cagggaaacg agttgcatat cagctgggga
       61 aaagttggta cccaagggca aagccaggta aaaaattttg ctgatgttgc agcagcggaa
      121 aaagcgaaac tgaagcttat cgcggagaaa acgaaaaaag gctatgtgga aaatgcttca
      181 gcaaacgtgc atatcccccc cattaccaaa gccactcctg aagttgaaac ttcccctgag
      241 agtaaaaacc aacgcccctg gctggctgat gatgcggtca ttcggctaac tgacgatatt
      301 aatcgatttg cttttcctca tcgctctcgc cccagagaga tcaattatct gcacaaagat
      361 ggtcaaatat ggcagctcat cgcgaataac accaaggcat atgatcccgc caacaattac
      421 cgttcttatc cagaaaactg gcagcaggct tttgctgagt tacaaatgcg agttcagggt
      481 aatcaacaga cagggagtgt gcaatctgat gcagcattgc tctggagttt ctggaactct
      541 tactctgcag atgaactgat cgatgactta gtcattcgct gtggtctgga aagtgccgtt
      601 gaaattgccc ttctcgctct tcaacttaga tataaaccag taaaagccga cgtcaccacc
      661 atcattcccc ctgatctcaa agcagaatcg ctaccgaact ggcatcaacg tctatgtcat
      721 catctttcac tcgcttcaga agatgagtgg caacgctgtg tagataaggt actcgcggct
      781 attccctcgc tatccccagc acggcaacct tttgcggcgt tactgatccc tgaacgcccg
      841 gatatcgcca atgctatggc gctacattat gcagatcaaa acgttccggc gatgacctgg
      901 ttaagtatga tggtaagcga tgatgttgcc ctggccaccc tggaaaaata tggtttccca
      961 ccgctatata atgattttcg caaatatctg gctacgttgc tggcaaataa tggaatgcgt
     1021 ggtgtaagcc ggatacttct caagctacct gtcgactatc ccgtaaaata cacggacctt
     1081 ttcactcaca tacacgccaa tgctgaagat cttgtcaaat ggctatggaa aacgaatcac
     1141 ccagatgcga ttcaaattct gatcctcggt gtaaatggca agaaaaagca cctggaatac
     1201 ttaagcaaag cctgccaaaa acatcccgct gcggctattg cggcttatgc tactttgctg
     1261 gcaatacatg aagataaaga gtggcgtaaa gcgcttgtca aactgattac cgccacgcca
     1321 gagttagtat gcgacgtcat tccctgggtt aatgctaaag cggcaggcat cttatctgaa
     1381 tgtcgtccgc agccagtagc tgaagaatgt gaatatgcca ctgtggatat gttgccggag
     1441 ctgcttgttt cgccgccctg gatgaccaaa gagaaaaaga aaaatacgcc tgtgtttgat
     1501 cttccagtgc ttccagttcc ttctgtttct gatgtcactc cagagattac caaaaaactg
     1561 acccgtactt accttgtcac ccacttccag caaatagccc aacagcaggc gacaaaacaa
     1621 acattgttta ccgacctgcc gccgatcaaa aaagcaagct gggagaaaca cttaataccg
     1681 cttacaccgg agcaacaaat actgtggcat cttggttttg aaaaatggcg ggaaagcgga
     1741 gaaaaaatat acgaaaaaat tccggcgcca caaagcgccg ttgatgccct gctacgcttc
     1801 gattttccag cactcaatgc tgaatttgtg caatatcata acaatgcata caaaagctgg
     1861 aatctcattg ccctctgtta cctgcctggt caacaggcca tttcttttct taaccagata
     1921 gtcaaagaag ataactactc tggtgaggga aatattctgg caatttttgg tagcgccgcc
     1981 atacctgcat ttatggcctg cctgcaaggt gatccacgac gtctgtgctt cttccccttt
     2041 ttcctcggtg tcagtgaact ggccttaccg atggcgcaac aattacagaa aaaaatgtct
     2101 tatgaagatg cgcgaaactg gctaactgat tatccacgcc atgccgccgc cgggctactt
     2161 cctgtggccc tgggcaaaaa aggaaaagat cgtgattgtg cccgccaggc gcttcgtctg
     2221 ctggtgaatt taaaccagcg agaaacgatt gaggaaatcg cgcaaggata taatcaacct
     2281 gatgttttag ctgcgctggc aacattgttc gatagcgacc cactggaaga atatcccgct
     2341 aaaatcgccc cactgtccgg cttttatcag ttcaccttgt ggcgcagacc acggcttaaa
     2401 agtaacaacc tgcctctgtc agatgacgct atgcgccacc tcggcactat gctgagcttt
     2461 cctcgcgaca ttaccgccta cgctgggctg gatatcatta gagaaatctt cacccgcgaa
     2521 tcactggctg aatttggctg ggatctgtat accgcctgga cagaagctgg cgcacccgca
     2581 aaagaaaact gggcatttac ttcgcttggg attttaggca atgacgacac cgcgcgtaaa
     2641 ttaaccccgc ttatccgcgc ctggcctggg gaatcccagc ataaacgggc ggtgtccggg
     2701 ctggatgtat tagctgatat tggcagcgat gttgcgctaa tgctgcttaa tggcatcgcg
     2761 cgaaaaatta aattcaaagc attacaggaa catgcccgcg agaaaatcaa catagttgct
     2821 gaaaaccgtg ggctgactat ggctgaactg gaagaccgcc tggctccaga tttagggctt
     2881 gatagcagtg gctcgctgat actggatttc ggcccccgca aattcaccgt tggttttgac
     2941 gaaaccttaa aacctgtagt gtgcgatgca aacggcaaag tcctgaaaga tttacctaag
     3001 ccaaaccaga gcgatgataa aacactggca actgacgcag ttaatctctt caaacagttg
     3061 aaaaaagatg ttcgtgctat cgccagccag caaattaccc gtctggaaca ggctatgtgc
     3121 cagcgcagac gctggacggc agagcagttc cgcctgtttc tggtggagca tccgctggta
     3181 cgtcacttaa cccgacggct gttatggggg gtttataacg atgaaaacgc tcttatcacc
     3241 tgttttcgcg tggcagaaga cagcacttac agcgatgcgc aggatgagtt attcacgctg
     3301 ccagcaggaa acatcggtat tccgcatgtg ctggaaatat cccctgaatc cgccgctgca
     3361 ttcaggcaaa tttacgctga ttacgaactg cttccccctt tccaacagct cgagcgaggt
     3421 agctatcacc ttgctgataa tgaacgtaat actcacgaac tgacacgctg gcaaggacgg
     3481 ctttgccagg ccggacgcat tgtcgggctg gaacgcagag gctggcaacg tctggaagaa
     3541 agcggcagcg tttatgcgat gcgcaaaaca acccctcatg gcgatctcga acttgaaacg
     3601 gaaccttttt cattaattta tggtgaaacg gggtatggcg accagctacc ggttgaaagc
     3661 gtaaaaatca cctcgccaga tgatcgctac ggtaaacaat cctcactcac tttctccatg
     3721 ctcgacgaca tcaccgccag cgagctgatt aacgacatcg aatcactgtt tgattaaaag
     3781 gatttaacaa tgaaaaccta tatttatcag gacgaaaaat cacataaatt ctgggcggtg
     3841 gagcagcagg aaaacgagct gcatattacc tggggtaaag tcggtagtaa cgggcaaagt
     3901 caggtaaaaa gctttgctga tgctacggcg gcggaaaaag cggaactgaa gctgattgcg
     3961 gagaaggtga agaaggggta tgtggagcaa gtagacgcta acgctcactc acatgatgcc
     4021 tcagcaaaga aagtcctggt cacagaggaa aaagcctcaa taaatcgcca ggcagcaact
     4081 aatggtttac cctggctggc agacaacgat cccattattc ttcccccgaa tattgcccgc
     4141 catgccctca gccaccgcct ttggccggga gagccagtaa aaaaaccaac caaacccgag
     4201 aaacaaaatt taatttcccg attggcaaat cgtacttatc attattataa aaatctgact
     4261 ttcgattaca gctcatgctc tgatgaatgg gctgatgcga ttagccaggc aatcagctta
     4321 attaaggccc aaaagactga gatacccgcg cgtactatgg cggtacttac ttttctggaa
     4381 atatgttttt gtcagggctg gaataatgaa acctcatccg agttgctgga tgaaattgta
     4441 agcaccttcg gtatcgaata cgcaactgaa actgtcatct tctggtggca aat
//
LOCUS       DAZKFU020000312         4471 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZKFU020000312 DAZKFU020000000
VERSION     DAZKFU020000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.314"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            170..1327
                     /gene="acrE"
                     /locus_tag="ACHOQQ_RS22120"
                     /old_locus_tag="pgaptmp_004424"
     CDS             170..1327
                     /gene="acrE"
                     /locus_tag="ACHOQQ_RS22120"
                     /old_locus_tag="pgaptmp_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGU4472585.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1339..4443
                     /gene="acrF"
                     /locus_tag="ACHOQQ_RS22125"
                     /old_locus_tag="pgaptmp_004425"
     CDS             1339..4443
                     /gene="acrF"
                     /locus_tag="ACHOQQ_RS22125"
                     /old_locus_tag="pgaptmp_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HGU4472586.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1076 a         1089 c         1188 g         1118 t
ORIGIN      
        1 atattaatct ttttttgtgt ttatgtgcct tgagacgcct gtattcataa ctattcctta
       61 catcgacgaa tgataatttg taggatagcg aactgtattt ttctttctgc gagttaacgc
      121 gttgcctttt tgggtaaata acgcgctttt ggttttttga ggaatagtaa tgacgaaaca
      181 tgccaggttt ttcctcctgc cctcctttat tctgatctcc gcggctttaa tcgccggttg
      241 taacgataag ggagaagaga aagctcacgt cggtgaaccg caggttaccg ttcatattgt
      301 aaaaacggcc ccgttagaag ttaagactga attaccaggc cgcaccaatg cttatcgtat
      361 agccgaagtt cgcccacagg ttagcgggat cgtactgaat cgcaatttca ctgaaggcag
      421 cgatgtgcaa gcaggccagt ccctgtacca gatcgatccc gcgacctatc aggcaaatta
      481 tgacagcgcg aaaggcgaac tggcgaaaag tgaagccgcc gccgccatcg cgcatttgac
      541 ggtaaaacgt tacgttccgc tcgtgggtac gaaatacatc agccagcagg agtacgacca
      601 ggccattgct gatgctcgcc aggccgatgc cgccgtgatt gccgcaaaag ccacagtcga
      661 aagcgctcgc atcaatcttg cttataccaa agtgactgcg ccaattagcg gacgtatcgg
      721 caaatcgact gtgaccgaag gcgctcttgt cactaatggg caaacgactg aactggcgac
      781 tgtccagcag ctcgatccta tctacgttga tgtgacccaa tccagcaacg attttatgag
      841 gctgaagcaa tcagtagagc aaggaaattt gcataaggaa aacgccacca gcaacgtaga
      901 gttggtcatg gaaaacggtc aaacctatcc cctgaaaggt acgctgcaat tctccgatgt
      961 gaccgttgat gaaagcaccg gctccataac cctacgtgct gtcttcccta acccgcaaca
     1021 tacgcttttg ccgggtatgt ttgtgcgtgc acggattgat gaaggcgtcc aacctgacgc
     1081 cattcttatc ccgcaacaag gcgttagccg cacaccgcgt ggtgatgcaa ccgtgctgat
     1141 tgttaacgat aaaagtcagg ttgaagcgcg ccctgtcgtt gccagtcagg cgattggcga
     1201 taaatggttg attagtgaag gactgaaatc tggcgatcaa gtcattgtca gcggcctgca
     1261 aaaagcgcgt ccgggagagc aggttaaagc cactaccgat acccccgcag atactgcatc
     1321 gaagtaaggt aatctgacat ggcaaacttt tttattcgac gaccaatatt tgcatgggtg
     1381 ctggccatta ttctgatgat ggcgggcgca ctggcgatcc tacaattgcc cgtcgctcag
     1441 tatccaacca ttgcaccgcc tgcggtttct gtttcagcaa actatccggg cgctgatgcg
     1501 cagaccgtgc aggatacggt gacgcaggtt atcgaacaga atatgaacgg tatcgataac
     1561 ctgatgtata tgtcctccac cagcgattcc gccggtagcg tgacaattac ccttaccttc
     1621 cagtccggga ccgatcctga tatcgcgcaa gtgcaggtgc agaacaaact ccagctcgcc
     1681 acgccgttgc tgccgcagga ggttcagcag caggggatca gtgttgaaaa gtccagtagc
     1741 agctatttga tggtggcggg ctttgtctct gataacccag acaccacaca ggacgatatc
     1801 tcggactatg tggcctctaa cgttaaagat acgcttagcc gtctgaatgg cgtcggtgac
     1861 gtacagcttt tcggcgcaca gtatgcgatg cgtatctggc tggacgccga tctgctaaac
     1921 aaatataaac tgacaccggt tgatgtgatt aaccagttga aggtacagaa cgatcagatc
     1981 gctgccggac agttgggcgg aacgccagcg ttaccagggc aacagttgaa tgcctcgatt
     2041 attgctcaga cgcggttaaa aaatccggaa gaattcggca aagtgaccct gcgcgtaaac
     2101 agtgacggct cggtggtgcg tctgaaagat gtcgcacggg ttgaacttgg cggtgaaaac
     2161 tataacgtta tcgctcgcat caacggaaaa ccggcggcgg gcctgggtat taagctggca
     2221 accggtgcga acgctctcga taccgcgaaa gccattaagg caaaactggc ggaattacag
     2281 ccattcttcc cgcagggaat gaaggttctc tacccttatg acaccacgcc attcgtccag
     2341 ctttctattc acgaagtggt aaaaacactg ttcgaagcta ttatgctggt gttcctggtg
     2401 atgtatctgt tcttgcagaa tatgcgagca acgctgatcc ccaccattgc ggtacccgtg
     2461 gtgttgttag ggacgtttgc catcctcgcc gcttttggtt actccatcaa cacactaacg
     2521 atgttcggga tggtgcttgc catcgggctg ctcgtcgatg atgcgatagt ggtggtggag
     2581 aacgtcgagc gcgtgatgat ggaggataag ctcccgccaa aagaagcgac ggaaaaatca
     2641 atgtcgcaaa ttcagggcgc actggtgggt atcgcgatgg tgctgtcagc ggtatttatt
     2701 ccgatggcat tcttcggcgg ttctactggg gcaatttatc gccagttctc tatcaccatc
     2761 gtttcggcaa tggcgctttc tgttctggtg gcattgattc ttacccctgc gttatgtgca
     2821 acgctgctta aacccgtctc tgctgagcat cacgaaaata agggcggttt cttcggctgg
     2881 tttaatacca ccttcgatca tagcgttaac cactacacca acagcgtcgg caaaatcctc
     2941 ggttccacag gacgatattt actgatctat gcgctgattg ttgcaggaat ggtagtgttg
     3001 tttttacgtc ttccgtcttc cttcttacct gaagaggatc agggtgtctt tctgaccatg
     3061 attcagttac ccgctggcgc gacgcaagag cggacgcaaa aagtgttgga tcaagtgacg
     3121 gattactatc tgaagaacga gaaagcgaac gttgaaagtg tctttacggt taacggcttt
     3181 agcttcagcg gccaggcaca aaacgccggt atggccttcg tcagtctgaa accgtgggaa
     3241 gagcgtaatg gtgacgaaaa cagtgcggaa gcggtaatcc atcgtgccaa aatggaattg
     3301 ggcaagatcc gcgacggttt tgtcattcca ttcaacatgc cagccattgt tgaactgggc
     3361 acggcaacgg gtttcgactt tgagttaatt gatcaggctg ggctgggtca cgatgcccta
     3421 acccaggccc gtaaccagtt gcttggtatg gcggcgcaac atcctgccag cttagtcagc
     3481 gtgcgtccta atggcctgga agacaccgcg cagtttaaac tggaagttga ccaggaaaag
     3541 gcacaggcat taggtgtttc actttctgac atcaatcaga ccatttcaac ggcgctgggt
     3601 gggacttacg ttaacgactt catcgaccgt ggtcgcgtga aaaaggtgta tgttcaggcg
     3661 gatgccaaat tccgtatgct gccagaagat gtcgataaac tttatgtccg cagcgccaac
     3721 ggcgaaatgg tgccattctc tgcctttacc acttcacatt gggtgtatgg ctctccgcga
     3781 ctggaacgct acaacggtct gccgtcaatg gagattcagg gggaagccgc gccaggaacc
     3841 agttccggcg atgccatggc gttgatggaa aaccttgcgt caaaattacc tgcgggcatt
     3901 ggttatgact ggacgggtat gtcgtatcag gaacgcttat cgggaaacca ggctcccgct
     3961 ctggtagcaa tttcctttgt ggttgttttc ctgtgccttg ctgcactcta tgaaagctgg
     4021 tcaattcctg tctcggttat gttggtcgtg ccgttaggga ttgtcggcgt gctgctggcg
     4081 gcgacactct ttaatcaaaa aaatgacgtc tactttatgg tgggcttgct aacgacaatt
     4141 ggcttgtcgg ccaaaaacgc tattttgatt gttgagttcg ctaaagatct catggagaaa
     4201 gagggtaaag gtgttgttga agcgacacta atggcagtac gtatgcgtct gcgtcctatc
     4261 ctgatgacct ctctcgcctt tattctcggc gtattaccgc tggctatcag taacggtgcc
     4321 ggcagtggcg cgcagaacgc agtgggtatc ggggtaatgg gcggaatggt ctctgcaacg
     4381 ttgctggcaa tcttcttcgt accagtgttc tttgtggtga tccgccgttg ctttaaagga
     4441 taaatcagaa acataaaggc gctttcgggc g
//
LOCUS       DAZKFU020000313         4374 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZKFU020000313 DAZKFU020000000
VERSION     DAZKFU020000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.315"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..833)
                     /locus_tag="ACHOQQ_RS22130"
                     /old_locus_tag="pgaptmp_004426"
     CDS             complement(<1..833)
                     /locus_tag="ACHOQQ_RS22130"
                     /old_locus_tag="pgaptmp_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4472587.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLENMDAGELFFWQVGSAAM
                     LSWFVLAISRKKLPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITAVIFSLESV
                     FILILSVILFSVKINSPLHFILLLTGAMAGSIMVALPDINDRIDNITGYILIVSGVLS
                     ASFYVVISSRLIVNFEPVTLLTGQLTFSFILACFFIVLTGTSLQLPIESVFLVFLSGI
                     LQYFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITIL
                     CV"
     gene            complement(826..1008)
                     /locus_tag="ACHOQQ_RS22135"
                     /old_locus_tag="pgaptmp_004427"
     CDS             complement(826..1008)
                     /locus_tag="ACHOQQ_RS22135"
                     /old_locus_tag="pgaptmp_004427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472588.1"
                     /translation="MIFNWNVLFYVSLEKFPVGNPYYGEIPGIPVDEEAKFFNPFPPR
                     AFENIPLYTEEITKYD"
     gene            complement(1048..1248)
                     /locus_tag="ACHOQQ_RS22140"
                     /old_locus_tag="pgaptmp_004428"
     CDS             complement(1048..1248)
                     /locus_tag="ACHOQQ_RS22140"
                     /old_locus_tag="pgaptmp_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472589.1"
                     /translation="MKLDISVKYFLNIMIADFIIPAPEKISTTWHSDTKMNRFACPIH
                     STNSDYYSGFYHTDVLIPGELA"
     gene            1367..1714
                     /locus_tag="ACHOQQ_RS22145"
                     /old_locus_tag="pgaptmp_004429"
     CDS             1367..1714
                     /locus_tag="ACHOQQ_RS22145"
                     /old_locus_tag="pgaptmp_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HGU4472590.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLSMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1732..2322)
                     /locus_tag="ACHOQQ_RS22150"
                     /old_locus_tag="pgaptmp_004430"
     CDS             complement(1732..2322)
                     /locus_tag="ACHOQQ_RS22150"
                     /old_locus_tag="pgaptmp_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472591.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDSGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNREG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(2322..2579)
                     /locus_tag="ACHOQQ_RS22155"
                     /old_locus_tag="pgaptmp_004431"
     CDS             complement(2322..2579)
                     /locus_tag="ACHOQQ_RS22155"
                     /old_locus_tag="pgaptmp_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472592.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            3148..3561
                     /locus_tag="ACHOQQ_RS22160"
                     /old_locus_tag="pgaptmp_004432"
     CDS             3148..3561
                     /locus_tag="ACHOQQ_RS22160"
                     /old_locus_tag="pgaptmp_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472593.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            3563..4345
                     /locus_tag="ACHOQQ_RS22165"
                     /old_locus_tag="pgaptmp_004433"
     CDS             3563..4345
                     /locus_tag="ACHOQQ_RS22165"
                     /old_locus_tag="pgaptmp_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4472594.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDTWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFLMPESDAVTMLKNRQA"
BASE COUNT         1137 a         1061 c         1097 g         1079 t
ORIGIN      
        1 acgcataaaa tagttataaa aacgcctagt ccctgaataa atgatatatc ttcctgcagg
       61 aagaaccagg acaacagcaa tgctaccaga ggaataaaat agagcattgc tcctgctatg
      121 tgaacgggaa tccagcgtag ggaataaaga tagaaacaaa aagccaaaaa atattgtaaa
      181 attcctgaca aaaacacaag aaatacagat tctattggca gttgcaggga tgtgccagtt
      241 agaacaataa aaaaacaagc aagaataaaa gagaaagtca actgcccggt taacaacgta
      301 acaggctcaa aattcacaat aagtcgtgat gagataacaa cataaaagga tgctgagagt
      361 acacctgaaa caataagtat atatcctgta atattatcaa tgcggtcatt gatatcaggt
      421 agggcaacca ttatactgcc tgccattgct ccagtaagca ataatataaa gtgtaatggt
      481 gagttaattt taacggaaaa taatataaca gatagtataa gaataaatac tgactcaagt
      541 gaaaaaataa cagctgttat accagcagaa ataaatttta ggccatagag agtgaatgta
      601 tacgccagaa atggctcaaa aattcctgtt gaataggcaa ggattgattt ttttgttacc
      661 gggagttttt ttctgctaat tgcgagaaca aaccatgata acattgcagc actaccaact
      721 tgccagaaaa acaactctcc ggcatccatg ttttcgagac cttttttgct catcactatt
      781 gctgctcccc atgaaaaagc agcaagaaat gcaaacataa ttcccttaat catatttagt
      841 tatctcctca gtataaagag gtatattttc aaacgctctt ggtggaaatg gattaaaaaa
      901 ctttgcttct tcatccacag gaatacctgg aatttctcca taataaggat tgccaacagg
      961 aaacttttcc agtgagacat aaaacaatac attccagtta aaaatcatag gatcattact
     1021 atcaacatca tggattatga tggcgtccta agccagttct ccaggaatta atacatctgt
     1081 atgataaaaa cctgaatagt aatcactatt agtagaatgt atagggcaag caaagcgatt
     1141 cattttagta tctgagtgcc atgtggttga tattttttct ggtgcgggaa tgatgaaatc
     1201 tgctatcata atattaagaa aatactttac agatatatcc agtttcatct ggtttctcct
     1261 ttattgttat cgtgtaccgg tgcatgacaa cataatcaca gtatgataat aataatcaat
     1321 aacaataagc tgtgtcacgt ttacatcatg gaacggaggt gctcctgtga cctttgagca
     1381 gaaaaaagcc cgggccattg ccctgatgga cagcaaaaag atgtggcgga gcaattatgc
     1441 ccctcctctt ctgcgtattc tctggcggct gggtatccgg cttccgccat taccctttat
     1501 gccgttctgg caggtcacgc tgtcgatggg gagcttatgg ggcatttcct ggggatgtgc
     1561 aatgtggttt atctactggg ggccgtcagg aatggtggca ggtgaggcca ttattataag
     1621 tatcacgggt ggttttttgt tcggactgct cacggcctct ttccactggt ggcgcaggaa
     1681 agttaaccgg ctgccaccat gggatgatgt gtaaaccgtg caggcatgtt tttacggggt
     1741 gatgctttca ttaacctgca cacgaacctc cattcctgca tcgtgacagg cctgcagcca
     1801 ctgcgccact tccagcggat cgccctcccg gcgtaccacc ctgccttctc tgttccataa
     1861 ctgcagacag gtgctgccgt cgagacgtac cacaaaatcc ccacggcagg cctgatacgt
     1921 tatgccgttc agctgcaccg ccagatcagt ggcagaagtg ccgtctgcgg gcagacctgc
     1981 tgccgggatc agcggcagta cgacgacttc tgccgcacgc tgttctgccc ggaggcggtc
     2041 ctgttctgcc agcagcagag gctgtgccac ggcccggccg gcgtccgtca gctccactgc
     2101 cagttgcagg ttcggagcgc gcagggtacg cagccagccg gaatcctcca gacgacggca
     2161 ggaggcgcgc aggtttggcc cgtagaccgg ggcttccccg cttcgctcca gcacccgttc
     2221 tatgtcccgc gtcagcacgg ggcctggccg tttttcatca agggcagcca gcacaatcag
     2281 cactctccgc tgcagtggcg acggtcggcg gctctgggtc attacctgtc atcctccggc
     2341 cagatgaggt ttctgaggcg ctgaaagtcg gcgtcaatga gctcctgtct ctttatggca
     2401 ttcattgagg catagccatt tatggccagt tcatgccaga agatcagcac ggctccagct
     2461 ttggctgact cgcagagata aatcgcgccc tggctgtcca gattgcattc ccggatgcgt
     2521 ttttccgcaa accgggtcag ttgttcataa ctgagtgaaa acaccctttc ttcatccatc
     2581 ctgcgcgctc catctgataa tgttctgaca ggtaaaaatc aaattctata tgatttttgt
     2641 gttatcgatt tatgataata tcagatatcg tttcattact tatgattgaa gtcatatctg
     2701 atatgaattt atgtgtgttg cttaattatc atatcgcctg cccgtttccc gccgcgcaga
     2761 tccggtttcc ggctgttcag gatcttcctc tccccccaca caacgccacc tcctgtcagc
     2821 acaacatgta gtgctgaatt cagctgctga tgacactata tgttgtgtca tctccctgac
     2881 ctgtgatgcg ccgcgcaggg gcggaaaaca gcgatatgat tatttcctca gcgtggtaca
     2941 cttccggaaa gttgtgatat tccggaaagt cggatctgac ggaaacggct ctccggtaat
     3001 ttaacggcgt ggttatatgg atgcttgtta tgatggtgat gatgataacg gcatgatgtt
     3061 atcagacggc gtgacggtaa gggcagtgat gatggatgac gttatcgcat gaccgtccct
     3121 gcccggaaaa gaaaaaagga gtcacccatg ttttttattg agaatgaagg tcaggctgtc
     3181 gccggaacgg attactggca gtctgtacag gcgcaggccg gatatgtcta cctcagctgg
     3241 aatgccggcg cagccaggct gcttgtcccg gatgcggcaa aacatttact cagggagatg
     3301 cggggggctg agtacgtcat catcagtaag ggaacgctgc atggccgcga tgcgctggaa
     3361 ctggtatttg aagacggcag cgatgcgccg tttgtgatcc acatgctgag tgagcagtgc
     3421 gatcgcctgc tccccgaaaa caaccaggga gggggttttg ttgtcaccgt ctggacgcgt
     3481 ggcggtaacc agctccgtta tccgggaaag taccgtgttg tggaaaacct gcccgacgtt
     3541 tccccgtgga gtgaacactg atatgcagca cctgccggca ccgatccacc atgcccggga
     3601 tgccgctcag cttcctgttg ccatcgatta tccggcagcg ctggcactcc gccagatgtc
     3661 gatggttcat gatgaactgc caaaatacct gctggcccct gaagtgagcg ccctgctcca
     3721 ttacgtcccg gatctgcgcc gcaagatgtt gctggccaca ctgtggaaca ccggtgcgcg
     3781 cattaatgaa gcactggcgc tgacgcgggg ggatttttcg ctcacgcctc cgtatccgtt
     3841 tgtgcagctg gccactctga agcagcggac agaaaaagcc gccaggacgg caggaagaat
     3901 gcccgccggt cagcagactc accggctggt tccgctttcc gacacctggt acgtcagcca
     3961 gctgcagacg atggtggcca cactgaaaat ccccatggaa cggcgtaata aacgaacagg
     4021 caggacagag aaagcgcgga tctgggaagt gacggacaga acggtcagga cctggattgg
     4081 ggaggcggtt gccgccgctg ccgctgatgg tgtgacgttc tctgtcccgg tcacgccaca
     4141 tacgttccgc cattcctatg cgatgcacat gctgtatgcc ggtataccgc tgaaggtcct
     4201 gcagagcctg atggggcata agtccatcag ctcaacggag gtctacacga aggtgtttgc
     4261 actggatgtg gctgcacggc accgggtgca gtttttgatg cctgagtccg atgctgtcac
     4321 aatgctgaaa aacaggcagg cataaatcat aagtcataat tatgaattgt gatt
//
LOCUS       DAZKFU020000314         4338 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZKFU020000314 DAZKFU020000000
VERSION     DAZKFU020000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.316"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..420
                     /gene="ydiV"
                     /locus_tag="ACHOQQ_RS22170"
                     /old_locus_tag="pgaptmp_004434"
     CDS             <1..420
                     /gene="ydiV"
                     /locus_tag="ACHOQQ_RS22170"
                     /old_locus_tag="pgaptmp_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU4472595.1"
                     /translation="NYAGELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLG
                     NLGAGNSTMKAVFDGLFTRVMLDKSFIQQQITHRSFEPFIRAIKAQISPCCNCIIAGG
                     IDTAEILAQITPFDFHALQGCLWPAVPINQITTLVQR"
     gene            483..1919
                     /gene="selO"
                     /locus_tag="ACHOQQ_RS22175"
                     /old_locus_tag="pgaptmp_004435"
     CDS             483..1919
                     /gene="selO"
                     /locus_tag="ACHOQQ_RS22175"
                     /old_locus_tag="pgaptmp_004435"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGU4472596.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSNSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1923..2114)
                     /gene="hemP"
                     /locus_tag="ACHOQQ_RS22180"
                     /old_locus_tag="pgaptmp_004436"
     CDS             complement(1923..2114)
                     /gene="hemP"
                     /locus_tag="ACHOQQ_RS22180"
                     /old_locus_tag="pgaptmp_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGU4472597.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2246..3292)
                     /gene="aroH"
                     /locus_tag="ACHOQQ_RS22185"
                     /old_locus_tag="pgaptmp_004437"
     CDS             complement(2246..3292)
                     /gene="aroH"
                     /locus_tag="ACHOQQ_RS22185"
                     /old_locus_tag="pgaptmp_004437"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGU4472598.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3449..4282)
                     /gene="ppsR"
                     /locus_tag="ACHOQQ_RS22190"
                     /old_locus_tag="pgaptmp_004438"
     CDS             complement(3449..4282)
                     /gene="ppsR"
                     /locus_tag="ACHOQQ_RS22190"
                     /old_locus_tag="pgaptmp_004438"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGU4472599.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         1053 a         1041 c         1141 g         1103 t
ORIGIN      
        1 aattacgcag gcgaactatt aaaatatccg tttattgaac tgttgattaa tgagaattac
       61 ccgcatctca atgaaggaaa agataatcgc ggtcttttat ccttatcgca agtgtatccc
      121 cttgtgttag gtaatttggg agcaggcaat agcacaatga aagcggtttt tgatggtctt
      181 ttcacccgcg tcatgctgga taaaagtttt attcagcaac aaatcacgca ccgctccttc
      241 gaaccgttta ttcgtgcgat caaggcgcaa atttcccctt gttgcaattg catcatcgcg
      301 ggaggcattg atacggcaga aatattggca cagattaccc ccttcgattt ccatgctctc
      361 cagggatgcc tgtggcctgc cgttcccata aatcagataa cgacgttggt tcagcgataa
      421 ccctcctgtt tgctggtgtt taagacgaga gtaaccgtct acactatcaa acaggaggat
      481 ctatgaccct gtcttttatt acccgctggc gcgatgaatt gccagcaacc tatacagcac
      541 tttcccctac gcctttaaat aatgcccggc tgatttggca taataccgaa ctcgctaaca
      601 cgctgagtat tccatcgtcg ctgtttaaaa atggcgcagg tgtctggggc ggcgaaaccc
      661 tactgcctgg tatgtcacca ctggcgcagg tttacagtgg tcatcagttc ggcgtctggg
      721 cgggccaact gggtgatggg cgcggcattt tacttggcga acaactgctt gctgatggca
      781 ctacaatgga ctggcatctg aaaggtgctg gcctgacgcc ttattcgcga atgggtgatg
      841 gacgggcggt tttacgttcg acgatacgag aaagtctcgc cagtgaggcg atgcattatc
      901 tgggcattcc gacgacccgc gcgttaagta tcgtcaccag caattcgcca gtgtatcggg
      961 aaacggtgga gccaggcgcg atgctgatgc gtgtggcacc aagtcatctg cgctttggcc
     1021 atttcgaaca tttttactat cgacgcgagc cggaaaaggt tcgtcagttg gctgactttg
     1081 ccattcgtca ttactggtca catcttgaag atgatgagga caaataccgt ctctggttta
     1141 acgatgttgt cgcacgtacc gcatcgttaa ttgcccaatg gcagacggtc ggctttgctc
     1201 atggggtgat gaataccgac aacatgtcgc tgctgggact gacgcttgat tacgggccgt
     1261 ttggttttct taatgattac gaacccggtt ttatttgtaa tcactcggat catcaagggc
     1321 gttacagctt tgacaatcaa cctgctgtcg cgttgtggaa tttacagcgt ctggcgcaga
     1381 cattgtcacc atttgttgcc gtagatgccc tgaatgaggc cctggacagc tatcagcagg
     1441 ttttgttgac gcattatgga caacggatgc ggcagaaact gggcttcatg acggagcaaa
     1501 aagaggataa cgcgctactg aatgaattat tcagtctgat ggcgcgagag cgcagcgatt
     1561 atacccgcac atttcgcatg ctgagtctga ccgagcagca cagcgcggcg tcaccgctac
     1621 gtgatgagtt tattgatcgt gcggcatttg atgactggtt tgcccgttat cgagggcgtt
     1681 tgcaacaaga cgaggttagc gatagtgagc gtcagcagct gatgcaaagc gttaaccccg
     1741 ctctggtatt gcgcaactgg ttggcgcaac gggcgattga agcggcagaa aagggtgata
     1801 tgacggaatt gcaccgcctg catgaggcgt tgcgaaatcc tttcagcgac agagatgatg
     1861 actatgtcag tcgtccacct gactggggta aacggctgga agtcagctgc tcgagttaaa
     1921 ggctacttgg tcaacagcag cttgccagcc tgagttttac ggagcagata ttcttgcccg
     1981 tcatgatcga taatcagttt gccatccgga cctaacagtg tctggctgga aattcgccga
     2041 ataggctgcg gggaagcggt cttgtgattg gcatccgttt caggcgtgag ttttctgcta
     2101 tccgtataac gcataaaaat taataataat tgatataaca atgattctta ttatcaatat
     2161 tatggctgac cagagcaagc agtaatgtgt tgagaagaca acaagttgcg cagaaatgcc
     2221 catgccgtca ggaatgggca cgcattcaga agcgggtatc taccgcagag gcgagttttt
     2281 cgaccaggcg ttcggtatct tcccagccca gacacgggtc ggtaatggat tgaccgtaag
     2341 tgagcggctg actgccgacg attttttgcg ttccttcgcg caggaaactt tccgccataa
     2401 ttccagcaat cgccgtagag ccattgcgga tttgctgaca aatatcctca caaacttcta
     2461 actggcgacg gtgctgcttc tggcagttac cgtggctgaa atcgaccacc agatgttcag
     2521 gtaaatcaaa ctcgtgcagc gtatcgcagg ctgcggcgat atcatcggca tgataattcg
     2581 gttttttgcc gccacgcata ataatgtggc catacgggtt gccgctggtc tgatagatgg
     2641 tcatctgacc atttttgtct ggcgagagga acatatggct ggcgcgggct gcgcggatag
     2701 catccacagc aatccgcgtg ttgccatcgg taccattttt aaaacctacc ggacaggaga
     2761 gtgccgaagc catttcacgg tggatctgac tttcggtagt acgtgcgcca atcgcgcccc
     2821 aactgattaa atcagcaata aactgaccgg tcaccatatc gaggaactca gtcgcggtag
     2881 ggacacccag ctcatttacc tgtaaaagta atttgcgcgc cagctccaga ccgtgattta
     2941 cccgatagct gccgtttaaa tctggatcgg agattagtcc tttccagccg acaacagttc
     3001 gtggtttttc aaaataggtg cgcattacga tttccagccg tgactggtac tggttgcgca
     3061 gcgactgcag acgggtggcg tactccattg cagcggtgag atcgtggatc gagcaggggc
     3121 caataatgac caacagtcgc ttatcttcac cattgagtat tttttcaatt ctgcggcggg
     3181 agtcggtgac atgggtggcg acgccaggcg ttacgggata ccgtagcgcg agttcggcgg
     3241 gcgttaccag gctctcaata cgcgcagtac ggagttcgtc agttctgttc attacaagtc
     3301 tcagaaattt gttgcttcta cggcaggact gccgggaagt gatgcgcatc acgataaacc
     3361 aatcgctgct gatttcaagt gcgaggtgtg tcacacctcg ccgggtggtt agcatgataa
     3421 caaaaaaata agctaatgca ctagttctct agtacattcg gcgactaagg cccatgatat
     3481 cgaggatctt ggtggcaatc tcttctaccg aataattggt actgttaatc cacgggatct
     3541 gatttttacg gtacaaggct tccacttccg cgacttccat cctgcactga cgaagcgagg
     3601 catagcgact gttctcccga cgttcctcgc gaatcgccgc cagacgttcc gggtcgatag
     3661 tcaggccgaa caatttatgc tgaagcggtt tgagcgacgc gggtagcacc agattatcca
     3721 tatcgtcggc aataaagggg tagtttgccg cgcggatacc aaattgcatt gccagataca
     3781 gactggtggg ggttttacca cagcgagaaa caccgagcag gatcacctga gcctgatcca
     3841 gattgcgcaa cgaaatgccg tcatcgtggg cgagggtgta atcaatcgcc gcaatgcgcg
     3901 catcatattt attgagatta ttagggttaa ggccatgggt acgatgagca atcggcgttg
     3961 gatccagttt catctcttgt tgtagcgggg caaccagcgc ctgaacgata tcctggcaaa
     4021 agccttcact ttgcaagatg atggcgcgaa tctccggcaa cacgatggag tagaagacca
     4081 gcgggcgcac gcctgtctgg tgataaattg cgtcaatctg atccttcact gcccgtgcac
     4141 ggctctcatt ttcgacaaac ggcagcgtga tgctgctgat agtgacggga aattgtgaca
     4201 ttactgcgtg tcctaatacc tccgcagtta ttgccgtacc atcagaaata taaaaaacgt
     4261 ggcgatcaac agcattatcc attttgttct tcccgtgatg cagacataat tctatgaaag
     4321 cataaattaa aaacgcac
//
LOCUS       DAZKFU020000315         4286 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZKFU020000315 DAZKFU020000000
VERSION     DAZKFU020000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.317"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..4286
                     /locus_tag="ACHOQQ_RS22195"
                     /old_locus_tag="pgaptmp_004439"
                     /pseudo
     CDS             1..4286
                     /locus_tag="ACHOQQ_RS22195"
                     /old_locus_tag="pgaptmp_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1028 a         1164 c         1344 g          750 t
ORIGIN      
        1 agcaatacct gaagtcccac agggggaacc ctcatggggt gatattgcca ctgacctgct
       61 tgaatcggca gcggaaaaag cagtaccact ggcgaaggcc tgggggaacg ctgttatcac
      121 cctgacggac agcaatgcca gttttatgga tcgcgtcagt gcaggcgcat cgcttctgtt
      181 tcccagcggc ccggtattaa tggaatttgc cacgatggtg ggcgggcgtg gcgaaatcaa
      241 aaaagatgtg gatttcccgg aagccggtga ggacacggcg ctctgcgaca aggagaacaa
      301 accaccgagg atagcccagg gcagcagcaa cgtctttatc aacaatcagc ctgccgcgcg
      361 ccagggggac aaactggagt gcagtgcggc gatcgtggaa ggttcgccgg acgtctttat
      421 tgggggtgag caggtcacct atctggatat ccagccggag ttcccgccat ggcagagaat
      481 gatcctggga ggaataacga tagccagcta tcttctgccg ccagcaggac tgctgggaaa
      541 actggggaat ctggcgaaac tgggcaaact gggaaacctg ctggggaaaa gcggaaagct
      601 gctgggcgca aaactcggcg cgttgctggg gaaaacaggt aagtcgttaa aaagtattgc
      661 caataaagtc atcagatggg taacagatcc tgtcgatccg gtaaccggcg cgtactgcga
      721 cgaacgtacc gacttcaccc tgggccagac cctccccctc tccttcaccc gtttccacag
      781 ttcggtactg ccactgcatg gcctgacggg cgtgggctgg agtgactcct ggagcgaata
      841 cgcctgggtg cgtgaacagg gaaaccgggt ggatatcatc agcctgggag ccacgctgaa
      901 cttcgccttc gacggtgaaa gtgatacggc ggttaacccg tatcacgccc agtacattct
      961 gcgccgccgt gatgattatc tggagctgtt cgacagggat gcactgagca gccgcttctt
     1021 ttatgacgcc tttccgggaa tgcgtctgcg ccacccggtg actgacgata ccagcgatga
     1081 ccgcctggca cacagccccg cagaccggat gtacatgctg ggcgggatga gcgacaccgc
     1141 cagcaaccgc atcacgtttg agcgcgacag ccagtaccgg atcacgggtg tcagtcacac
     1201 cgacgggatc cggcttaaac tgacgtacca cgccagcggc tacctgaaag ccattcaccg
     1261 cacggataac ggcatacaga cgctggcgac ctacgaacag gatgcgcggg ggcggctgac
     1321 agaagcggat gcgcggctgg actaccacct gttttatgag tacgacgctg cggaccggat
     1381 catccgctgg tccgataacg accagacgtg gagccgtttc acctacgatg aacagggccg
     1441 gtgcgtgacc gtcaccgggg cggagggcta ttacaacgcc acgctggact atggggacgg
     1501 ctgcaccacc gtgacggacg gcaagggcgc tcaccgttat tactatgatc ctgacggcaa
     1561 tattctgcgg gaagaagcgc cggacggcag caccaccacg tatgaatggg atgaatacca
     1621 tcacctgctg gcccgccact cccctgccgg gcgggtggag aaatttgaat acaacgccgc
     1681 acatggtcag ttaagctgtt atacggcggc agacggcgcg gagtggcagt actgctatga
     1741 tgagcgcggc ctgctcagca acatcaccga ccctgccggg cagacgtgga cgcagcagtg
     1801 tgatgaacgc ggcctgccgg tgagtctggt gtcgccacag ggcgaagaga cccggctggc
     1861 atacaccgca caggggctgc tgtcgggaat attccgccag gatgaacggc acctgggcat
     1921 agagtacgac caccacaacc ggccggaaac actcaccgac gtgatgggcc gtgaacacca
     1981 caccgaatac agtggtcacg acctgccggt gaagatgcgc ggccccggcg gttagtcagt
     2041 gcggttgcag tggcagcagc accataaact gagcggcctt gagcgggcag gaaccggcgc
     2101 agaaggattc cgctatgacc gccacggcaa cctgctggcg tacacggacg gtaacggcgt
     2161 tgtctggaca atggaatacg gcccgtttga tttgccggtg gcgcgaacgg acggtgaagg
     2221 ccaccgctgg cagtaccgct acgataaaga cacgctgcaa ctgacagaag tcattaaccc
     2281 gcagggcgag tcttatcttt atattctgga caactgtggc cgggtgacgg aagaacgtga
     2341 ctggggcggc gtggtctgtc gttaccgcta tgacgccgat ggcctgtgta ccgccagggt
     2401 taacggcctg gaggaaacta tcctctacag ccgggacgcc gcaggccgcc tggcagaaat
     2461 catcacgccg gaaggcaaaa cgcagtatgc gtatgacaaa tccggcaggc tgacgggtat
     2521 cttcagcccg gacggcacat cacagcgcac cggctatgat gaacgcgggc gggtgaatgt
     2581 caccactcag ggccgacggg ccattgaata ccactacccc gacgaacaca ccgtcatccg
     2641 ctgtatcctg ccaccggaag atgaacgcga cagacacccc gatgaatccc tgctgaaaac
     2701 cacgtaccgt tataacgccg ccggagaact gacggaggtc attctgccgg gggatgagac
     2761 gctgacgttc agccgtgatg aggcgggacg tgaagtgctc cggcacagta accggggttt
     2821 tgcctgtgag cagggctgga atgcagccag ccagcttgtc acccagcgcg ccggattttt
     2881 cccggaggaa accacatggg gcgggctgct cccctcactg gtacgggagt accgttacga
     2941 cagcgcgggc aatgtgtcgg ctgtcaccag ccgggaagat tacggacggg aaacacggcg
     3001 ggagtaccgg ctggaccgga acggtcaggt cacggcggtg acagcctcag gcaccgggct
     3061 gggctatggc gaaggcgatg agtcctatgg ctatgacagt tgtggctacc tgaaggcgca
     3121 gtctgcgggc aggcaccgga taagtgaaga gactgaccag tatgccggag gccaccggct
     3181 gaaacaggcc ggaaacacgc agtatgacta tgacgccgca ggccggatgg tcagccgcac
     3241 aaaacaccgt gacggctacc gcccggaaac agagcggttc cggtgggaca gccgggacca
     3301 gctgaccggg tattgcagcg cacagggtga acagtgggaa taccgccacg acgccagcgg
     3361 cagacgaaca gagaaacgct gtgacaggaa gaaaatccgc ttcacgtacc tgtgggatgg
     3421 cgacagtatt gcggaaatcc gggaataccg cgatgatgaa ctgtacagcg tacggcacct
     3481 ggtgtttaac ggctttgagc tgataagcca gcagtgcagc cgggtacggc agccgcaccc
     3541 gtccgtggcc ccgcagtggg tgacgcgaac gaatcatgcg gtgagcgacc tgacgggccg
     3601 cccgctgatg ctctttaaca gtgaaggtaa aaccgtctgg cggccggggc agaccagcct
     3661 gtgggggctg gcactcagcc tgcccgcaga caccggctac ccggacccgc gcggggagcg
     3721 ggacccggaa gcggaccccg gcctgctgta tgcgggacag tggcaggatg cagaatcggg
     3781 gctgtgctat aaccggttcc ggtactacga gccggaaacc gggatgtacc tggtgagtga
     3841 tccgctgggg ttgcagggag gggagcagac ttaccggtat gtgccgaatc cttgtgggta
     3901 tatcgatcct ttggggctgg ctatatgtca gttagcccgc tggacgaaat gggggagtga
     3961 gcaaagcaac atatctgatg ttttgaactc attagggaat agagcactta aatatgctaa
     4021 tggtgattgg ataaaatcag aggctgcatt caataaatac ataaacatga taaataaaag
     4081 actagaatta acaggtagta aatttagagt tgagattcaa ccagccataa aaaatggaga
     4141 gcgagttcct gcgacaacga atggaccatt taaagtaaat ggtaagtgga catccggcac
     4201 tcattataca ggtggttcca aacgtctaga tgccggtatt attgatatca catctcctac
     4261 aaaccaatat ggattacatc cagtta
//
LOCUS       DAZKFU020000316         4266 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZKFU020000316 DAZKFU020000000
VERSION     DAZKFU020000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.318"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(133..801)
                     /gene="artM"
                     /locus_tag="ACHOQQ_RS22200"
                     /old_locus_tag="pgaptmp_004440"
     CDS             complement(133..801)
                     /gene="artM"
                     /locus_tag="ACHOQQ_RS22200"
                     /old_locus_tag="pgaptmp_004440"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGU4472600.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(801..1517)
                     /gene="artQ"
                     /locus_tag="ACHOQQ_RS22205"
                     /old_locus_tag="pgaptmp_004441"
     CDS             complement(801..1517)
                     /gene="artQ"
                     /locus_tag="ACHOQQ_RS22205"
                     /old_locus_tag="pgaptmp_004441"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGU4472601.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1524..2255)
                     /gene="artI"
                     /locus_tag="ACHOQQ_RS22210"
                     /old_locus_tag="pgaptmp_004442"
     CDS             complement(1524..2255)
                     /gene="artI"
                     /locus_tag="ACHOQQ_RS22210"
                     /old_locus_tag="pgaptmp_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGU4472602.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2273..3001)
                     /gene="artP"
                     /locus_tag="ACHOQQ_RS22215"
                     /old_locus_tag="pgaptmp_004443"
     CDS             complement(2273..3001)
                     /gene="artP"
                     /locus_tag="ACHOQQ_RS22215"
                     /old_locus_tag="pgaptmp_004443"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGU4472603.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3219..3734)
                     /locus_tag="ACHOQQ_RS22220"
                     /old_locus_tag="pgaptmp_004444"
     CDS             complement(3219..3734)
                     /locus_tag="ACHOQQ_RS22220"
                     /old_locus_tag="pgaptmp_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4472604.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         1107 a         1074 c         1048 g         1037 t
ORIGIN      
        1 agaatttatg cactatgtag gccggataag acatccacgc cgcatccggc acaggcaccg
       61 tgctgatgtc tgatgcgacg ctggcgcgtc ttatcagacc tacaaacccc ccggcgaatg
      121 tacgcagcta cattaattcc gccgttcgaa tgccagtgct ttgcgctcga tcagacgcat
      181 catcagcgtc agcaggccgt taacgaccag gtaaataatc cctgccgcac cgaacaccat
      241 tacatcgtag gtgcgtccgt acaacaactg gctgtatccc atcacttcca tcagcgtaat
      301 ggtgtaagcc agagaggtac ttttgaacac cagcaccact tcgttggaat aagaagagag
      361 cgagcgttta aaggcatacg gcagcaggat cgccagcgta tcttttttgc tcattcccag
      421 ggcgctacag gactgccact gaccttctgg gatcgcacga attgcaccgt aaaacagctg
      481 cgtggtatac gccgcactgt tcagcgacaa cgcaatcagc gcacataacc acggttctga
      541 caacaaatgc cacagtgccg gatactcctg caacgtcggg aactgacccg gcccgtaata
      601 aatcaggaag atctgcacca gcagcggtgt accggtaaac agcgtgatat aaccccgcac
      661 cagccacacc agcaccggcg ttttcagcgt caggatgatg gtaaaaatca acgccagaat
      721 cagtgccaca atcagcgagg caacggttag cgtcaggctg gtgtgcagcc ctttcatcag
      781 ttcgggtaaa tactcaaaca ttagctgggc ctccgctcaa aacgtgtcgc gcgcaggtca
      841 atgcgtttga gaatgtactg actgagcagg gtgatcacca ggtaaatcgc cgccgccaca
      901 atgtaccagg taaatggttc ctgggtacga gtagcgatgc ttttggtttg tagcattaaa
      961 tcattcacac taatcaaact gaccagcgcg gtatctttca gcagcaccag ccactggtta
     1021 ccgaggccag gcagcgcatg acgccacatc tgcggcatca ccagacggaa aaagatagcc
     1081 gatttcgaca gccccagcgc ctgaccagat tcccactggc ccaccggtac cgctttcaac
     1141 gcgccgcgca gcgtttgcga ggcataggcg gcatacagca gtgacagagc gatgacacca
     1201 caaaggaacg gactcacgtc gaagttctca atgtccatct gcaccgggat ctgcacgaac
     1261 ccaagattga tggtgaagcc atccgaaagc gtcagcagca gctgtgagga gccaaaatag
     1321 ataaacagca ccaccagaat ttctggcagg ccacgtagaa tggttaccag cgctgaacct
     1381 gcccacgcga caggacgcca ttttgccgac tcccacaccg caaagaacat cgccagcgcc
     1441 agtccgacaa tcaatgcaca aacggcaagg ccgacggtca tcccggcggc gcttgctaaa
     1501 ggaaaaaatt cattcatcag gaattacttc tggaaccatt tgttgtagat ggtttcgtaa
     1561 gtgccatctt tcttcacttt ttccagcgca gtgttgagtt tctgctgcag ttcagtgttg
     1621 ccctgacgta ccgcgatgcc gaggccagta ccgaagtaat ctttatcggt cactttatcg
     1681 cccactgccg ccagtttcgg gttatctttc agccattcgg ttaccactgc ggtgtcacca
     1741 aatacgctgt cgatacgacc attttgcaga tccagttttg cgttctggta gctgtcatac
     1801 ggaacggtgg tgatttccgg gtgcttatcc ataatgaact tctggtgtgt cgtcccgttc
     1861 tgaacgccga cttttttgcc tttcagctga tcaacactgg tgtatttgcc ttgctgaccc
     1921 acaaacaggg cagagttgtc atagtacggg gtggtaaaca gcacctgctt ttcacgctcc
     1981 ggagtgatat ccatgcccgc catcacggct tctacgcgac ggaatttcag gcttgggatc
     2041 aggctgtcaa acgcctggtt agagaaagta caggttgcat caatctcttt acacagtgcc
     2101 tgtgccaggt cgacgtcaaa accaacgatc tggttgtttg catcaatcga ttcaaacgga
     2161 ggataggagg cttcggtagc aaaacgaatg gtttcggcag ctgtggcgga aagactaaaa
     2221 cctgcaatta acgcggcaat cagaactttt ttcattgttg ttatcccgaa tcttagtgag
     2281 agagatagtt tttaaatgct tcggtttgcg gctcggtaaa gcagctcgcg tcgccttgtt
     2341 ctacgatatg accattttcc atatacacca ctcggctggc ggttttacgc gccacttcaa
     2401 cttcgtgggt gacgatcacc tgggtaatat tcgtttctgc cagctcacga atgatgctga
     2461 cgatttgtgc ggtaatttcc gggtccagtg cggcggtcgg ttcatcaaac agcagtacct
     2521 gcggttccat cattaacgca cgggcaatag caacacgctg ctgctgacca ccagaaagat
     2581 gcagcgggta acgatcgcta taaggtttga gacgcagacg ttccagcagt ttttctgcac
     2641 gggccagcgc ctgatcttta ctcaacccca gtacacggca gggcgcttca atcaggtttt
     2701 gctgcacggt cagatgcggc cacaggttgt attgctgaaa caccatgcca acgttacgac
     2761 gcaaatcgcg aatcgctttg tctgagggtg ttttggtgaa atcgaaatgg ttgcctgcaa
     2821 tgttgagcgt accggagcgc ggcatctcaa gcagattgag tacacgcagc agcgagcttt
     2881 tacccgcgcc gctggggcca agtaacacca gcgtttcgcc ctgtgggcaa tccagcgtga
     2941 tatcgaacag cgcctgatgc gcgccgtaga agcaattaat gccgtttaat tgaatactca
     3001 ttgacactcg tatactggca gtctgatagc tattgaggtc gaagatagta cctttgacag
     3061 aataattatg caatatttct gctttaaaag ttaaaagcaa agcgcattat tcaataaaca
     3121 tagcacaaaa taacgggggc ggtggtcggc gagcataaat gtcggcattc ctcacgaaat
     3181 gccggacaat ttacggggtt tattggttga tcaaggcgtt agcgattctc gatggactga
     3241 cggagcgtac ccgccgtggc atgaacgcta ccgcctaagt agcgcacatc gtcgatcacc
     3301 caacactggc cttcctgaat cattaacact tcatcctgcc agccctggtc accctgcttg
     3361 agatccacgc gcaatggaat gttacgggca tcacgattag ggatagtcga tgcactggca
     3421 acgtgggcgc tgtctggcaa ggtggttcga ctggagaacg gatcgctgct taacaattca
     3481 cgatgactat tatcgcggct ggcatcgcta agtagggttg ccagtttgtc actcaaatag
     3541 gggcgcaggg cggtaatgtc attgctgcga tgttgaatgc ggtagtcata gaattgttgg
     3601 gccacgctgt caggcccccc ctggacacag gaaccggtgc ggggaccgtt atctttataa
     3661 gctggcgtga cggtggtaca ggcactcagc agcagtgcac atggaataag tagagatagt
     3721 gaacggtagc gcataatcat ttccttatta gcttgctgtc tgcgtcacac tacgaccaaa
     3781 cgctatcaga ttatttaata cattgttttt aatacaattc tgaaacaatc tgaacacctg
     3841 attgggtttg agtccattgc tggctaccca ctcaaggttg ctcgtagctg acttacttaa
     3901 attacccaca caatagacag tgtaaaccat ttgtgaggtt gagcgtgtta tatggctgaa
     3961 atgatcattt aaggtaaata tgatagttct gaaatggtgg tataaatcct cctgtagagg
     4021 ttaagagcat ctgcaagaag ccagtttcac atttataacg cgtgataaat gaacgcggtt
     4081 attgtctaat cattacaaat ttttcacatg cgataagaga tcctgctatt aagatgattg
     4141 ccaaaattac gttgtatttt tgaaaatgag cgaaatttga tagaaaattt atctgctgta
     4201 cgatttaatg aacttaatga tacagcatta caaaaacaat cgaagtttat aaaataacat
     4261 ttttat
//
LOCUS       DAZKFU020000317         4212 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZKFU020000317 DAZKFU020000000
VERSION     DAZKFU020000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.319"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..847
                     /gene="hyxR"
                     /locus_tag="ACHOQQ_RS22225"
                     /old_locus_tag="pgaptmp_004445"
     CDS             53..847
                     /gene="hyxR"
                     /locus_tag="ACHOQQ_RS22225"
                     /old_locus_tag="pgaptmp_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HGU4472605.1"
                     /translation="MKKPLVIISACQFTRLALESLIPADRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            1001..1762
                     /gene="hyxA"
                     /locus_tag="ACHOQQ_RS22230"
                     /old_locus_tag="pgaptmp_004446"
     CDS             1001..1762
                     /gene="hyxA"
                     /locus_tag="ACHOQQ_RS22230"
                     /old_locus_tag="pgaptmp_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HGU4472606.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVCNITYIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAENLPVMDSYSVFMNEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1804..4101
                     /gene="hyxB"
                     /locus_tag="ACHOQQ_RS22235"
                     /old_locus_tag="pgaptmp_004447"
     CDS             1804..4101
                     /gene="hyxB"
                     /locus_tag="ACHOQQ_RS22235"
                     /old_locus_tag="pgaptmp_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter HyxB"
                     /protein_id="HGU4472607.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADNAVSTTPVTLDKDKTT
                     LDQDVVINGDKKITAVTIETSDSDKDLNVTFGGHDITAASTVNQDFVEGVKVSGNKNV
                     VINATDSTITAQGEGTYVRTAMVIDSTGDVVVNGGNFVAKNEKGSATGISLEGAKGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSLLNSFEGDATDNITLAGSNIINGRIETIV
                     TAGNKTGIHTVNLNIKDGSVIGAANNKQTIYASASAQGTGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYTFNSGDKAGSNLDINLSDSSVWKGKVS
                     GAGNASVSLQNESVWNVTGSSTVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASS
                     ENTLDISGKASGDLRVYSAGSLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQG
                     ADGNFYFVKNTHKASNASSVIQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWI
                     QYFGGKQKHTTAGNASYDLDVNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSK
                     GDTEGYSFHAYLSRQYNNGIFIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAM
                     VKGGYTWEDGNGLFIQPYAKLSALTLEGVDYQLNGVNVHSDSYNSVLGEAGTRVGYDF
                     AVGNATVKPYLNLAALNEFSDGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAY
                     ASLDYTKGDDIENPLQGVVGINVTW"
BASE COUNT         1151 a          914 c         1065 g         1082 t
ORIGIN      
        1 acattttgaa tcaaaatgtc aggttttgta gcaatggcgt ggacgagtct gaatgaagaa
       61 gcctctggtt attatcagtg cctgtcagtt caccaggctg gcactggagt ctctcatccc
      121 agcggacagg tatatcgtac gggtgtacag caacgtcact acagaggttg aattcgtttt
      181 aaagacctcc tgtggctatc tgttggctga tatcccttcg cttccttcgg gggagttagt
      241 tcttctggct tgtcttgtcc gcttgcggag tgatgccggg caatggcagg tctgcctgac
      301 cggtgactcc gactggttcg ataatgctcc tgctgcaagt ctgtataacg gcttcccacg
      361 catcggcacc atgctgacag cgagtcgtta tcagaatcaa attatccgct ggttactacg
      421 tcctttgccc ggcgatatga gcgatttctg gctcctgact gcacgggagt tgagcgtgct
      481 taagattctg atgcagggca tgagtttcag tgagattgcc cgttatgaac aacgcagttc
      541 aaagaccctg catgcgattg ccacacgggc attaatgaaa cttggactgg gaactctgag
      601 cgatttcagg ctgctctata cgggatgcgg tgacagcaga gtgaaacgga atatacggct
      661 acatgcaaaa tatgtcggac aacattccgc tcatcgactc actcagtatg tgcagaacat
      721 ggtatccgga attctttatc cagagaaagt tgccgttggt gaatcaataa attctgacgc
      781 catatccgga aaagtacttt ccggacaaag aaaaaatatg tctaaaaaaa gaccaggatt
      841 gttatgacac agcagcgttg ttactacccc ccactgcggc gcgtacggac tcgctacagt
      901 gtaggggcca aaatatttct actgctctgg gctgcaggat tgggaggcac gctgtacagc
      961 ctgtacagcg tgctggcgta ctgacatctg tgagggaagg atgcgtttcg ttcttttctg
     1021 tccctcagga attgtacctg cacagttcgc agctctatca accggttctg tctgcaatat
     1081 tacctacatc agaacagaag aggagttacg caataaattg cggcacaggc cacagagtgt
     1141 cgtgatatcg gcagggcgac ctgccgaatg cgctgaaatg tggttccgat tttatcggga
     1201 tcactcattc gtggtggtgt tgtgcgtcgc gccctttttt ctgccaccgg atgtgagtat
     1261 ttctggcgtt ctcaaaaacc tgaggttact gaaaccagga atgtcagtag agcgtgtcat
     1321 cagtattgca aatgcctctg gtgggttttc aggactgaaa catgcagaaa atttgccagt
     1381 tatggacagt tactctgtct ttatgaatga ggtgaataac cgcacaaaga caatcgtcat
     1441 gtcagaacgc ttcccggaaa agcaaaagaa agtgttgtcg ttgcttcttg ccggacatag
     1501 ttgggaatac agtgcgcagt ttctcaaaac ggggataaga cagatatggc ttgccgagca
     1561 gtcgctaaaa aagcgttggg gaataccgga cagcatgtca ttgcgggaag ccctgctgct
     1621 gagcagcaat aaatttcacg gtgatggaaa tgcattagaa aaaacgaatg ccatgacatt
     1681 gcgtgaaaac ggaaatacca acaggtattc cgtggtggta aatgcaggga cgcaggcttt
     1741 atctctgcat aaatataaat aagtgggaaa ttgaaattag ttaccagaat ggagaatttc
     1801 ttcatgaaaa acagtaaggc attttaccgc agcgcattag cgacagctat tgttatggct
     1861 ctttctgcac cagcattcgc tgctgataac gcggtatcaa ctacaccggt tacattggat
     1921 aaagataaga caactctgga tcaagatgtt gttattaacg gtgataaaaa gattacagcc
     1981 gtaacaattg aaacgtcaga ttcagataaa gaccttaatg ttacttttgg tggtcatgat
     2041 attaccgccg catcaacggt aaaccaagat ttcgttgaag gtgtaaaagt tagtggtaac
     2101 aaaaatgttg tgattaatgc tacagactcc accatcacag ctcaaggtga aggcacctat
     2161 gtccggactg caatggtcat tgattcaact ggcgatgttg ttgttaatgg cggtaatttc
     2221 gttgcaaaaa atgaaaaagg tagtgcgaca gggatatctc tggaaggggc caagggaaat
     2281 aatgtcacgc ttaatggcac aaccataaat gctcaaggta ataagagttc cagcaacgcc
     2341 tccacggcaa tttttgctca aaagggtagc ttattgaata gttttgaagg tgatgcaacc
     2401 gacaacatta cccttgctgg ctcaaatatt attaatggtc ggattgaaac aatagttact
     2461 gccgggaata agacgggaat tcatacagtc aacctgaata ttaaggatgg ctcagtaatt
     2521 ggggcggcta ataataaaca aacaatttat gcctctgctt cggcacaagg cacaggttca
     2581 gcaacgcaaa atttaaattt gtctgttgct gattcaacca tctactctga tgtcctggcc
     2641 ctttctgaaa gcgagaattc agccgctacc acaacaaatg taaacatgaa cgttgcccgc
     2701 tcttactggg aaggtaatgc ttatactttc aatagcggcg ataaagcggg tagtaatctg
     2761 gatataaatc tttccgatag ttcagtctgg aaaggcaaag tttcaggggc aggaaatgcc
     2821 agtgtatctc tgcaaaacga gtctgtctgg aatgttacgg gctcctcaac tgttgatgct
     2881 ctggcagtaa aagacagtac ggttaatatc acgaaggcta cagtcaatac tggcacgttt
     2941 gcttctcaaa acggcactct gattgttgat gcctcttctg aaaacactct ggatatcagc
     3001 ggtaaagcga gcggtgactt gcgtgtttac agtgcgggtt cattggatct tatcaatgaa
     3061 caaacggcat ttatttctac cggcaaagac agcactctaa aagccacagg cacaacggaa
     3121 ggtggtctgt atcaatatga cctgacacag ggagctgatg gtaactttta tttcgtaaaa
     3181 aacacgcata aagcatccaa cgccagctcc gtgattcagg caatggcagc agctccggct
     3241 aacgttgcta atctgcaggc tgacacgctc tcagcccgtc aggatgctgt ccgtctgagc
     3301 gaaaatgaca agggcggcgt atggattcag tactttggcg gtaaacagaa acataccacc
     3361 gcgggaaatg catcctatga cctggatgta aacggtgtaa tgctgggtgg tgatacccgc
     3421 ttcatgactg aagatggtag ctggctggcc ggtgtggcga tgtcttctgc gaaaggtgac
     3481 atgaccacca tgcagagcaa aggcgacact gaaggttaca gcttccacgc ttacctgagc
     3541 cgccagtata acaacggtat cttcattgat actgctgcac agtttggtca ctacagcaac
     3601 acggcagatg ttcgcctgat gaatggtggc ggtaccatca aagctgactt taacaccaat
     3661 ggtttcggtg cgatggttaa aggcggttac acctgggaag acggtaatgg cctgtttatt
     3721 cagccatatg ccaaactgtc tgcgctgacg ctggaaggtg tggattatca actcaacggc
     3781 gtgaacgttc attctgacag ctataactct gtgctgggtg aggccggtac gcgcgtgggt
     3841 tatgacttcg ctgtgggcaa cgcgaccgtt aaaccttatc tgaatctggc cgcactgaac
     3901 gaattctctg atggcaacaa agtccgtctg ggtgatgagt ctgtcaatgc cagcattgac
     3961 ggtgcagcat tccgcgtggg tgcaggtgta caggctgata tcaccaaaaa catgggagca
     4021 tatgcaagcc ttgactacac caaaggtgac gacattgaga atccgctaca gggtgtagtt
     4081 ggtatcaatg tgacctggta agaataaagc agtcggttcc cctgtcgact gttatacccc
     4141 ctgcaggcag cagtatgctg cctgcaggct ataaatatcc agtattcatg atgctggctt
     4201 tttgctgata tt
//
LOCUS       DAZKFU020000318         4197 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZKFU020000318 DAZKFU020000000
VERSION     DAZKFU020000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.320"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..101
                     /locus_tag="ACHOQQ_RS22240"
                     /old_locus_tag="pgaptmp_004448"
     CDS             <1..101
                     /locus_tag="ACHOQQ_RS22240"
                     /old_locus_tag="pgaptmp_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016808261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HGU4472608.1"
                     /translation="TIRDGIDHYLAPSRAQPRSSAAVTGDYRRERP"
     gene            101..343
                     /locus_tag="ACHOQQ_RS22245"
                     /old_locus_tag="pgaptmp_004449"
     CDS             101..343
                     /locus_tag="ACHOQQ_RS22245"
                     /old_locus_tag="pgaptmp_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HGU4472609.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            420..734
                     /locus_tag="ACHOQQ_RS22250"
                     /old_locus_tag="pgaptmp_004450"
     CDS             420..734
                     /locus_tag="ACHOQQ_RS22250"
                     /old_locus_tag="pgaptmp_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HGU4472610.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            747..1109
                     /locus_tag="ACHOQQ_RS22255"
                     /old_locus_tag="pgaptmp_004451"
     CDS             747..1109
                     /locus_tag="ACHOQQ_RS22255"
                     /old_locus_tag="pgaptmp_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HGU4472611.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGVIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            1106..1768
                     /locus_tag="ACHOQQ_RS22260"
                     /old_locus_tag="pgaptmp_004452"
     CDS             1106..1768
                     /locus_tag="ACHOQQ_RS22260"
                     /old_locus_tag="pgaptmp_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HGU4472612.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            1768..2676
                     /locus_tag="ACHOQQ_RS22265"
                     /old_locus_tag="pgaptmp_004453"
     CDS             1768..2676
                     /locus_tag="ACHOQQ_RS22265"
                     /old_locus_tag="pgaptmp_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HGU4472613.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTVASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNAHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            2666..4153
                     /locus_tag="ACHOQQ_RS22270"
                     /old_locus_tag="pgaptmp_004454"
     CDS             2666..4153
                     /locus_tag="ACHOQQ_RS22270"
                     /old_locus_tag="pgaptmp_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HGU4472614.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEHQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
BASE COUNT          897 a         1027 c         1368 g          905 t
ORIGIN      
        1 agaccattcg tgacggtatt gaccattatc tggccccttc ccgggcgcaa ccccggtctt
       61 ccgcagccgt gaccggtgac taccggcggg agcgtccctg atggcgatga cggccctgca
      121 gaatacggcc ttccgggctg cttcaggtga agtggatctc agtgtgctgc atctcatcac
      181 gctgggattt ctgctggcag tcgtgttcct ctgggcagcg tggagtctga cggatgcctg
      241 ggcaggctgg cgtagcggtg atgtccggga tggtgtgttc ggctggttta tgttccgact
      301 cgtcctgctg ctgatctgca gtatctggat gtttgcgggt tgatttcagt gtcttgttta
      361 agtttcagga gaaaataatg cataaaaaaa tgcgcatctg tcgccaggcc tggtatgcca
      421 tggcggtgtt tctgtgtacc gggatcacac ctgctctggc tgatttgcct cagatagagc
      481 cgccaacatc cggtggaggt ggtggacttt tcggacagat caaaggctat ctccaggatg
      541 gtgttgtgct gtttggtctt gtgctcagtg cagttgcttt tgtgaaggtg gcggaagcgg
      601 cgctttccac cttctcagag gttcgtgacg ggaaggccgg atgggggcag tttggtgcca
      661 tcgtggtggt tggtgttgtt ctgctggtcg ccattatctg gctgctgggc aagtcggcct
      721 ccatcattct gtaaggagac atccccatgc agaccattcg ttttctgccc gatcgtctga
      781 acgtggagcc tgcggttttt cgtggtttta ccacaccgga actggggctg gcggccctga
      841 caggcgctgt ggcaggggtg atctggccgt taccgctgat tccgctactt ggctgggtga
      901 ttatcccgac agggatgttg ctgactcccc tgctgctggt ctggttcggt ggcgtctgga
      961 tcagccggct gaagcgggga aaacctgaca actggctctg gcagcatctg gcgaagaaaa
     1021 gcagtgcatg gggtggtggc cggaaaacat ttatccggga ggcgcatggc tggtccctgc
     1081 gccgtacccg tagcaggggg aaaaaatgag tcgttttcgt catgctctgc gggatcgcga
     1141 ccagcatatc ctgacactgc gtattttcag cggtgtgctg gccggaattc tgatgctggt
     1201 ctgtatcggg tggatgacgg caccggacag actgacaatt cacaatccgc cggatctgcg
     1261 ttccggcagt acccgtccct ggtgggaagt cccgccgtca acggtttatt cctttgcctt
     1321 ttatatcttt cagcaactga atgcctggcc gaagaacgga gaggtggact acccggcgaa
     1381 aattcatgcg ctctccgcct acctgacgcc atcctgtcgg gagattctgg agagtgatgc
     1441 ccgccgtcgc agtgatgcgg gggagctgct ggagcgtgtg cgcgtggtgt atgaaattcc
     1501 ggaacatggc tacgggggca ggtacaacaa aaatccggtg acggtggaag ggcgggataa
     1561 ctggcaggta cagctggatc tggtggcaga tgagtatttt catgctgagc cggtaaaacg
     1621 ggcacttgtc cgttatccga taaaggtggt gcgcatggag ggggatcctg aacgtaatcc
     1681 gttcgggctg gcgctggact gctacagcag tacaccacag cggctggaag cagcctcccc
     1741 ggcagccggg aagggggata atcagtgatg aacggagctg aacgttgttt ataccgtagt
     1801 attgtgatgg tcctgtcact gctggtttta ccactgagcg cacgggctga tgagctgatg
     1861 aaatgggagc gcattccctt acccgttacc ctgatggcag gacaggagcg ggttattttt
     1921 gcagataaaa atgtgcgggt gggcttcccg ccagcactga acggaaagtt gagggtacag
     1981 agtaccggtg gggcagtcta tctgaaagca gagagtgttt ttccgcagac gcgtctgcaa
     2041 ctgcaggatg tggagagcgg agaaattatt ctgctggatg tgacagcagc agaaaaaggg
     2101 cccacagagc ccgtacggat tgtgtatgag ggaagcgtca gtacagtcag tcaggctggc
     2161 acagtggcgt ctgcacgctc tgcagaacac aaaaaggtgg tacccggcgg ggatgacggc
     2221 tcgcaggccc tccgtcagcc ggtgaagtat gcagagcctt tacccgttct gttgacccgc
     2281 tatgcggcac agtcgctgta cgcaccgtta cggacgctgg aagcggtacc gggtatccgg
     2341 ccggttaatg cgcatctgcc cgggaaaatc accacactct atccgtcaga gccactgaca
     2401 gtcacgccac tggcagcctg ggctgcgggt gggcgtgcag ttgttgcact gaaactgacg
     2461 agtacggtaa gccgcaaaat tatccttgat ccgcgggcac ttcagggaaa attcattgcc
     2521 gcgactttcc agcaccgctg gctgggtgca gcaggtaccc ctgaggatac caccacgctc
     2581 tatgtggtga cggcagaacg gccggaacag gcgtttatcg cggagcctgc ctcccgtcgc
     2641 attccttcac ggggggaggt gaagcatgca gattaaatcc aatacgatcg tgaagtatgt
     2701 ggtgccctgt gtggtggtgg cggggatcct gacgggatta aaaagctgtg gaggagatac
     2761 cggggagccc tcgtctgcgg aaagctcagg aacgctggcc ggactgtcac cggatgaact
     2821 gaaggccctg ggcgtggaag gagacacacc tgaagatacc ctgcgcacac tgattgggcg
     2881 tatgaatcag gtgagggagc ggcaactggt actggacaaa cagaatgaaa agttgctgaa
     2941 agaaaatgaa atgctgcgca agcggggcac ggatgtttcc ggtcaggtca gtgaggcagt
     3001 ggcaggattg cagaaaaatt atgagcagtc gcagcgccag ttccggaatg aacagaacgc
     3061 gttaatgaca caggttcagt cactgacaga gcaactgaag cgtcagcagg agagtgctga
     3121 cgccggcgcc ggaggacagg agagcgatat tccagcaggt ctgggccttg acggactggg
     3181 gagcgtgaca ggatcggcag cctcaccggg aacggatggt ctgatgtggg tgacaccggc
     3241 cgatcaggtg caggatgaag cgtcgtcggt gggggataag agcggaccgc gttttccagt
     3301 ctcttttctg gatgacaatc cactgacccg tcagaaagcg gcttatgaac agcaggtgaa
     3361 gggatacagc aatgaacatc agaaggcgag cacccgggca gtgtataccc tgccggagaa
     3421 ctcgacgctg gcaggcagcc gggcgatgac agcgcttctg ggacgggttc cggtgaacgg
     3481 aaccgtgacg gatccctatc cgttcaagat cctggtgggt aaggataacc tgacggcaaa
     3541 cggaatagag ctgccggatg tggaaggtgc agtggtgtcc ggtactgcga gtggtgactg
     3601 gacgctctcc tgtgtccgtg ggcaggtgaa cagcatcacg tttgtttttg ccgacgggac
     3661 agtgcgtacg ctgccgtcat cagacgtgaa cggcagcagt aacgcacagg caaaaaataa
     3721 cagcagtaca ggaggcattg gctggatttc ggatgatgac gggatcccgt gtatcagcgg
     3781 aacccgtaaa tccaatgcct ccacgtatct gccgacaata tttgccctgt ctgcagccgg
     3841 cgctgcgggt gatgcactga gtgaggggca gtacacctca cagaataacg tcaatggcat
     3901 cacatcaaca ctgaccggca gtgccggaga ggcggcactt ggaaaagccc tgtccggcgg
     3961 gatgaatgaa accgcggaat gggtgaaaca gcgatacggg cagacgtttg atgccattta
     4021 tgttcccccg gggcagacgc tggcggtgca tatcacgcgt caactggcca ttgattatga
     4081 agatcagggg cgccgggtac gttacgactt cactctgccg ggggaagtcc acggtaccgg
     4141 tggtctggac tgatgtaagg agatcccgtg aaacgacagt tatctgtaat gaccgtc
//
LOCUS       DAZKFU020000319         4193 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZKFU020000319 DAZKFU020000000
VERSION     DAZKFU020000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.321"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(146..1378)
                     /gene="serA"
                     /locus_tag="ACHOQQ_RS22275"
                     /old_locus_tag="pgaptmp_004455"
     CDS             complement(146..1378)
                     /gene="serA"
                     /locus_tag="ACHOQQ_RS22275"
                     /old_locus_tag="pgaptmp_004455"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU4472615.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(1634..2293)
                     /gene="rpiA"
                     /locus_tag="ACHOQQ_RS22280"
                     /old_locus_tag="pgaptmp_004456"
     CDS             complement(1634..2293)
                     /gene="rpiA"
                     /locus_tag="ACHOQQ_RS22280"
                     /old_locus_tag="pgaptmp_004456"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGU4472616.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(2349..3245)
                     /locus_tag="ACHOQQ_RS22285"
                     /old_locus_tag="pgaptmp_004457"
     CDS             complement(2349..3245)
                     /locus_tag="ACHOQQ_RS22285"
                     /old_locus_tag="pgaptmp_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4472617.1"
                     /translation="MIDWNDYYYFALIADEGSYSQAALRAGVSKSVLSRRMAALEERL
                     GVRLIQRTTRRLALTAAGEQFAVECRALMAQEERARAVVMAAQESPQGMLRVSAPVFM
                     AETWFGEFVSEFIARWPLAKVQLLAINRPVDMVAEGIDIALVTASGALADSTLHYKKL
                     HTQADILVASSQWLARHPQVKAIEDLNHVDGILRLANNGGSQWQLINGEQEITLQPEA
                     RLQSNNLRVMLQAVLGGNGVALLPQFACKQGLANGELVRLFAPWSGIPRPLYALFAGR
                     KGMPAIARYFMDELTTRLANGV"
     gene            3346..3975
                     /locus_tag="ACHOQQ_RS22290"
                     /old_locus_tag="pgaptmp_004458"
     CDS             3346..3975
                     /locus_tag="ACHOQQ_RS22290"
                     /old_locus_tag="pgaptmp_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGU4472618.1"
                     /translation="MKILHIDSSPMLKHSSSRKLSQTLVEALCQHFPKATIIHRDVGL
                     TPPPHLREETFMALCDKAECGSAQIRQEVNGIHAAIEELASCDVLIIGAPMINHSVSS
                     GLKTWIDQVCQAGLTFRFTSAGAVGLLTDKPTFIVSTRGGIYCDEAHLALDHQESYLQ
                     ATLKLMGITNTHILRAEGVDKTQPGRELAEQNALAQIPELITQVFNHYL"
     gene            3992..>4193
                     /locus_tag="ACHOQQ_RS22295"
                     /old_locus_tag="pgaptmp_004459"
     CDS             3992..>4193
                     /locus_tag="ACHOQQ_RS22295"
                     /old_locus_tag="pgaptmp_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472619.1"
                     /translation="MSIKQNLFAALFLCTLTGHAFAATEIHKPDNNLEQLSKVSMSTH
                     NSNPAGVESQIKEKAEELQASNY"
BASE COUNT         1071 a         1119 c          980 g         1023 t
ORIGIN      
        1 ccccattaaa taaacacaac gcattgatct gactttgatt tattttctgg agcatactcg
       61 caaagtagaa tgcgcaacgg tgtggagaag ggataaaaaa acgggcaagt cagtgacctg
      121 cccgttgatt ttcagagaag gggtattagt acagcagacg ggcgcgaatg gtacccggaa
      181 tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcggcttca atatcaataa
      241 ccacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg acgccctgct
      301 cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg atgtgcatca
      361 gacgacgccc accgtgcagt ggcagcgaga cttccgggaa gttcaccgca gagagcgttg
      421 agccattgtc agaatacttg atcaatttac ccgcaacttc caggccaata ttctcctgcg
      481 cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat tcacacagcg
      541 gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg attgccgccc
      601 ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc acagtaccgc
      661 gcgaagcatt aatcagcagc gagccgggct tcattagtga aatttctttc gcgcccatca
      721 tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc atattcagca
      781 ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttattt tcaatatcat
      841 aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta ccaatatgac
      901 cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc gccagtttgt
      961 tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat agcagcagca
     1021 gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg ttaaataccg
     1081 ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttcctata cagaaacagc
     1141 caatagcgac cagcttttct gcggcgttga tcacgtcttc agttagatgg gtacgggatc
     1201 gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca tccagcgcgc
     1261 ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc gccttttggt
     1321 gcactccttc taccagcaga aacttaatct tgtctttctc cagcgatacc tttgccattt
     1381 acccaatcct gtcttttgaa atgttgtgtg cggatttgcg tccgcctttc aacatatcaa
     1441 aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat atcacgcaaa
     1501 ggtaattctt ggatgaatat gctggtttag aggatttatt tagagcaatc gacaatagcc
     1561 aggtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta acgggggagg
     1621 ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca atcagcgcaa
     1681 cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc gcatttatgg
     1741 cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg atcacgttgc
     1801 cattatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc accagctggc
     1861 gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat ttacccagaa
     1921 tatcaacctg cttggaagcg tctgcaatac agataaattt ttctgcaacc gaagcaatga
     1981 ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg ccgttgattt
     2041 catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca aaaacgtgaa
     2101 tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc ccttcaatct
     2161 ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct gtacctacac
     2221 caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct tttttcaatt
     2281 catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt atatacaaaa
     2341 aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa atatcgcgca
     2401 atggcaggca tcccctttcg ccccgcaaat aaagcataca acggtctggg tatgccgctc
     2461 cacggtgcaa acaggcgcac cagttcaccg ttcgcaagcc cctgtttaca ggcaaattga
     2521 ggcaataacg ccacgccatt cccaccaagt actgcttgca gcatgacgcg aaggttattt
     2581 gactgcaaac gcgcctcagg ctgcagcgta atctcctgtt cgccattaat taattgccac
     2641 tggctcccgc cattgttcgc cagccgaagg atcccgtcga cgtggttaag atcttcaata
     2701 gctttcacct gcggatggcg ggccagccac tgagaactgg caactaaaat atcagcctgg
     2761 gtatgtaatt ttttataatg cagcgtcgag tcagcaagcg cgccggaagc cgtaaccagc
     2821 gcaatatcaa tgccttcagc gaccatatcc accgggcgat taattgccag cagttgcacc
     2881 ttcgccaggg gccaacgggc gataaactca ctgacaaact caccaaacca tgtttcagcc
     2941 ataaacaccg gcgcgctgac gcgcagcatt ccctgtgggc tttcctgggc ggccatcacc
     3001 accgctctgg cccgttcctc ctgcgccatc agcgcccgac attcgacagc aaactgttcg
     3061 cctgccgccg tcagggccag acgccgcgta gttcgctgaa taagccgtac gccaaggcgc
     3121 tcctccagtg cagccattcg tcggcttaat accgatttcg acacgcccgc acgcagcgcc
     3181 gcctggctgt agctgccttc atcggcaatc agggcaaagt agtaataatc attccagtcg
     3241 atcatgattg ttctattttc acaacaatga agttcaatta tgtctattta atcaccatat
     3301 aaacaacagt taaacttctc tcatcgcaga aatgagagca agcccatgaa aattcttcat
     3361 atcgacagta gcccaatgct taagcattca agttcgcgga aattaagcca gacgctggta
     3421 gaagcgcttt gccaacactt tccgaaagcc acaattattc atcgcgacgt tggcctgacg
     3481 ccgccgcccc atttacgtga agaaaccttc atggcgcttt gcgataaagc agagtgcggg
     3541 agcgcacaaa ttcggcagga ggtgaatggg attcacgccg ctattgaaga actggcatcg
     3601 tgcgatgtgc tcattattgg cgcgccgatg attaatcaca gcgtttcatc tggcctcaaa
     3661 acctggatag atcaggtctg tcaggctggt ttaacgtttc gttttacttc cgctggtgcc
     3721 gttgggttac tgacggataa accgacgttt attgtctcga cgcgcggtgg tatctattgc
     3781 gatgaagcac atctggcgct ggatcatcag gaaagttacc tgcaagccac gctgaaatta
     3841 atgggcatta ccaatacaca tattttacgt gcggaaggcg tcgacaaaac ccaaccggga
     3901 cgcgaactcg ccgagcaaaa tgcattagca caaatccctg aacttattac acaagtattt
     3961 aatcattatc tttaatataa aaggagttat tatgtctatt aagcaaaatt tatttgccgc
     4021 gttatttctt tgcactctca caggacacgc atttgccgca accgagatac ataaaccgga
     4081 caataacctt gaacagttat caaaagtgag catgtctaca cacaatagca acccagcagg
     4141 cgttgaaagc caaatcaaag agaaagcgga agagttacaa gccagcaatt att
//
LOCUS       DAZKFU020000320         4155 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZKFU020000320 DAZKFU020000000
VERSION     DAZKFU020000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.322"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..480
                     /locus_tag="ACHOQQ_RS22300"
                     /old_locus_tag="pgaptmp_004460"
     CDS             <1..480
                     /locus_tag="ACHOQQ_RS22300"
                     /old_locus_tag="pgaptmp_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4472620.1"
                     /translation="KPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESVFWLNVKN
                     IPPSIDKKNTNVLEIAIKTRIKLFWRPAAVKIIPEQAIKNVKWSRQGNNLIVENPNPI
                     HINVMSVTVDDKDVSLNMIRPFETLTLPIPAGVAGKSLVWRFINDYGAISQPLKKNL"
     gene            502..3036
                     /locus_tag="ACHOQQ_RS22305"
                     /old_locus_tag="pgaptmp_004461"
     CDS             502..3036
                     /locus_tag="ACHOQQ_RS22305"
                     /old_locus_tag="pgaptmp_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4472621.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFVQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYGDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSTEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSSSNYHDDNNDNFGKEQIF
                     MFSMSIPLSGWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLIQMDAICR"
     gene            3048..4139
                     /locus_tag="ACHOQQ_RS22310"
                     /old_locus_tag="pgaptmp_004462"
     CDS             3048..4139
                     /locus_tag="ACHOQQ_RS22310"
                     /old_locus_tag="pgaptmp_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4472622.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGVCFNSDGATHMSNLSFGPLT
                     VAAANNHSGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLE
                     RTLNGLNYYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNL
                     DSGRSAKLSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQN
                     CELTPNQSIDVDFQKINSGEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASM
                     IAANRSADATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFY
                     SAPASLTGTKPAPDNGFTATATLVIEFTN"
BASE COUNT         1137 a          954 c         1004 g         1060 t
ORIGIN      
        1 aaaccgccat tttctgtgat acctcccgtt tctcgcattg aaccgcagca agagaaaata
       61 ttacgcattt taaaaacggc tggcgagtta ccaaaagatc gagagtccgt attctggttg
      121 aacgtgaaaa acatccctcc ttcaattgat aaaaaaaata ccaatgttct tgagatagcc
      181 ataaaaacaa gaattaagtt attctggcga ccagccgcgg taaagataat ccctgagcag
      241 gctattaaaa atgttaaatg gtcgcggcag ggtaataatc tcatcgtcga aaatccaaat
      301 ccgatccata ttaatgtaat gagtgtgacg gtggacgata aagacgtgtc gctaaatatg
      361 atccgccctt ttgaaacatt gacattacca attccggctg gcgtggcagg aaaatcgctc
      421 gtttggcgct ttattaatga ttacggtgca attagtcaac cactaaagaa aaacctttaa
      481 gcgccggata aggatatatc gatggcctct tcgcatcttt tcattacgct tgcatcgggc
      541 atatgtctgc tctgttccat atctgctttt gcccgggata gcttgttcaa ccccagatta
      601 ctggaactgg atcatcctgc ggataatatt gatattcacc agttcaaccg ttcgaatacc
      661 ttacctgcgg gaacatacaa agttgatgtg atgatcaacg gcatgctctt cgaacgccag
      721 gaagttaaat ttgtccagga taaccctgat gctgaactcc acccatgcta cgtggcgata
      781 aaaaacgtac tggcgaccta tggtataaaa gttgatgcga taaaatctct ggcgaatgtt
      841 gatgacaaaa catgcgtaaa tccagttccg ctgatcgacg gggctacctg gttactggac
      901 gccagtaaac ttgcattgaa tattactatt ccgcaaattt atctcaacaa tgcagttaat
      961 ggttatatca gcccttcccg ttgggatcag gggatcaatg ccatgatgat gaattatgat
     1021 ttttcggcat cgcataccat ccggtcaaat tatgacgacg acgatgacag ttattatctg
     1081 aatttgcgta atggtattaa tttaggcgca tggcgttttc gtaattacag caccctgaat
     1141 tcttatgacg gtaatgtgga ctaccattcc gtcagtaatt acattcagcg cgacatcatg
     1201 gcattacgta gccagattat gattggcgat acctggacgg caagcgatgt atttgatagt
     1261 acacaggtgc gtggcgtgcg gctgtatacc gatgacgata tgttgccctc cagccagaat
     1321 ggctttgcgc cagtggtaca tgggattgcg aaaactaacg ccacggtgat catcaaacaa
     1381 aacggctacg ttatttatca atcagccgta ccacagggcg catttgccct caccgactta
     1441 aacacgacca gtagcggcgg cgatctcgat gtcactatca aagaagaaga tggcagcgag
     1501 cagcacttta ttcagccatt tacttcactg gccattctca agcgtgaagg tcagaccgat
     1561 gtagacctta gcattggaga agtgcgcgac gaaagcggct ttacgcctga ggtcttgcag
     1621 ttacaagcaa tgcacggttt ccctttggga ataaccttgt atggcggaac acaactggca
     1681 aatgattacg cttctgccgc gctgggtatt ggtaaagata tgggggcgct gggcgcgatt
     1741 tcttttgacg tgactcatgc ccgctcgcag tttgactacg gcgataatga gagtggtcaa
     1801 tcgtatcgtt ttctctattc caaacgtttt gaagacacca ataccacctt tcgtctggtg
     1861 ggttatcgct actctacgga ggggttctac accctcaatg aatgggtgtc gcgacaggat
     1921 aacgattctg atttctgggt aacgggcaac cgtcgcagcc gcttcgaagg cacctggacg
     1981 caatctttca cgccaggctg gggcaatatt tatttaacat tcagtcgaca ggaatactgg
     2041 cagaccgatg aggtcgagcg tttattacag ttcggctata acaacaactg gcgaaacatc
     2101 tcctggaacg tttcctggaa ctatacggac tcgatcaagc gctcatcgag caactatcat
     2161 gatgataaca atgataattt cggcaaagaa cagattttca tgttctcaat gtcgataccg
     2221 ctatcgggct ggatggaaga cagctacgtc aactattcgt taacgcaaaa caaccaccat
     2281 gaaagcacga tgcaggtcgg tctgaacgga acgatgctgg aagggcgtaa cctgtcttat
     2341 aacgtacagg aatcgtggat gcactctcct gatgactcct acagcggcaa tgccggaatg
     2401 acctatgacg ggacttatgg ctcggtcaat ggcagctatt cctggagccg tgactcccaa
     2461 cattttgatt atggcgccag aggcggcgtg ctggtgcata gtgacggagt gaccttctcg
     2521 caggaactgg gcgaaacggt ggcattagtc aaagcgccgg gcgcagaagg cctgtccatt
     2581 gaaaacgcca ccgggatttc taccgactgg cgtggttata ccgtaaaaac gcagcttagc
     2641 ccatatgacg aaaaccgcgt ggcattgaac agcgactatt tctccaaagc caatattgaa
     2701 ctggaaaaca ccgtcatcaa cctggtacca acgcgcggtg cggtggtgaa agccgaattt
     2761 gtcacccatg tcggttatcg cgtgctattt aacgtccgcc aggtcaacgg taaaccaata
     2821 atgtttggcg cgatggcaac cgcctctctc gaaacgggca cagtcaccgg gattgtcggt
     2881 gataacggcg aactgtatct ctccgggatg cctgaaaaag gcgagttttt attgagttgg
     2941 ggacaagctg cggatgaaaa atgtaaggcg gcctatcaca tcacccataa acctgatgat
     3001 accagcctga ttcaaatgga tgcgatttgt cgctaaagga agcatgaatg aaaaaaatat
     3061 tatcagggtt gattctgctg ctttgctgtc cttatggttt cgccgctaat ggtgatggcg
     3121 tttgttttaa tagtgatggc gcaacgcaca tgtcaaattt atcatttggt ccgctgacgg
     3181 tggctgcggc gaataaccac tccggataca atattttcga ggcactgagc aacacgactg
     3241 gaacataccc ggtgcgctgt cactgtgatg acacgcatgg cggaccgggc caacaaacag
     3301 cattttttcc tatcttctac acgggggatg ccgcaccggg gcttgtgctt gagcgcactc
     3361 ttaatgggtt aaattactat gctctgaatg attatttatc ggtcggcgtg acgattttta
     3421 ttattaataa ccaatatgcg gccattcctt ttgaacactt atccaaccaa tccacctcac
     3481 cgcaacatac ctgtggagca ggtaataatg ggagcactgt aaatctggat tcagggcgct
     3541 cggcaaaatt atctttctat gttcggcatt ctattactgg cacggtgaca atacccacaa
     3601 cggaagtcgc ctggttatac gcgggcatgt ccgatcattt tcccaaaacg acccccgttt
     3661 ctaaagtgac aattcgcgga caattaacgg ccccgcagaa ctgtgagtta acgccaaatc
     3721 agagcatcga tgtcgatttt caaaaaatta atagcggtga gttctcctca acggcgggtt
     3781 caattattgc agaacgaaag attaaaaccg aagtcacagt atcctgtacc gggatggaag
     3841 acgtaaggtc tacggaggtg gtgagtgcgt cgatgattgc ggcaaacaga agtgccgatg
     3901 ccaccatgat cgtgacgagt aatccggatg tgggaattaa gatttttgat aagaacgacc
     3961 gtccagtgaa tgtggatggg ggcaacttac ccgctgatat gggtgctatt agtcgattag
     4021 gaaaaaccga tggtagcgta acgttttatt cagcgcccgc cagtctgacg ggcacaaaac
     4081 cagcgcctga taatggattt accgccacag ccacgctggt tattgaattt actaactaat
     4141 cttgtgttag taaaa
//
LOCUS       DAZKFU020000321         4146 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZKFU020000321 DAZKFU020000000
VERSION     DAZKFU020000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.323"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            227..835
                     /locus_tag="ACHOQQ_RS22315"
                     /old_locus_tag="pgaptmp_004463"
     CDS             227..835
                     /locus_tag="ACHOQQ_RS22315"
                     /old_locus_tag="pgaptmp_004463"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGU4472623.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="ACHOQQ_RS22320"
                     /old_locus_tag="pgaptmp_004464"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="ACHOQQ_RS22320"
                     /old_locus_tag="pgaptmp_004464"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGU4472624.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..>4146
                     /gene="selB"
                     /locus_tag="ACHOQQ_RS22325"
                     /old_locus_tag="pgaptmp_004465"
     CDS             2321..>4146
                     /gene="selB"
                     /locus_tag="ACHOQQ_RS22325"
                     /old_locus_tag="pgaptmp_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGU4472625.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDA"
BASE COUNT          926 a         1043 c         1232 g          945 t
ORIGIN      
        1 gctcatcctt gtattatcgg gttatttgtc cgctgacagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tctgtcagat aagaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat tgggaggtga caaaccatca acattttctg ccctttttcc
      181 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcattac
      301 tttcgaattt attaatgaac tgccctataa cgcggacaat ggcgtggcgc aatttaaccc
      361 gttagggaaa gtgccagtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat
      421 catcgccgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccttt
      481 ggagtcgttg cgggtgcgca aaattgaggc gctggccgac ggcattatgg atgctgggct
      541 ggtgtcggtg cgtgaacagg cgcgtccggc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 acttaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggctggtg tgtcgaccgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgc tgcgtcagat gcttgacgaa gcgcgagaag
     1081 tgattcgtgg cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaggcg cagagcgcgc tgcgtccggt gataaacctg acgggaaccg
     1201 tgctgcatac caatcttggg cgagctttac aggcggaagc tgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc
     1321 gcgatcgggc gctggcgcag ctgctgtgcc gcattaccgg cgcggaagat gcctgtatcg
     1381 ttaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg
     1441 tggtggtttc tcgcggcgaa ttggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     1501 tgcgtcaggc aggctgcaca ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     1621 acagcattca ggggttcacc aaagcgatag acgaagctga actggtggcg ctcggcaaag
     1681 agctggatgt tccagtggtg actgatttag gcagtggctc gctggtcgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gtttctccgg cgacaagttg ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 cccttgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2101 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgccgatcg
     2401 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     2461 gccggatggt cgtgtgcctg gttttatcga cgtacccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 cgtgatggca cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     2641 gacagtggcg ctgaccaaag ccgatcgcgt ggacgaagcg cgtgttaatg aggttgaacg
     2701 ccaggtaaag gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt taccagaacg
     2821 tgaacacgcc agccaacata gtttccgcct cgcgatcgac cgcgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 atggctgact ggtgtaaata aaccgatgcg cgtgcgtgcg ctgcatgcgc aaaaccagcc
     3001 aacagaaacc gctcatgccg ggcagcgtat cgcgcttaac atcgcggggg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgtg ccgccagagc cgttcacgcg
     3121 ggtgattgtc gagcttcaaa cccatacgcc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtta cgggacgcgt ttcgctgctg gaagataacc ttgccgagct
     3241 ggtgttcgac acgccgctgt ggctggcgga taacgatcgc ttagtgttgc gcgatatctc
     3301 ggcccgcaat acgctggcgg gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa
     3361 acgtaagccg gaatatctgc aatggctggc gtctcttgca cgggcgcaga gcgatgccga
     3421 tgcgttatct gttcatctgg aacgcggagc ggttaacctt gcggatttcg cctgggcgcg
     3481 ccagctcaac ggcgaaggga tgcgcgaact gctgcaacag ccgggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc ccgctggcag cggaaaattc ttgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctggtcct gggcgcgaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct ggtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atccacagcc atcacggctg gttgcatctg ccagatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg
     3841 gtgggtgcgt gacctggcaa aagagacggg aaccgacgag caggcaatgc gtctgacgct
     3901 acgccaggcg gcccaacagg ggataattac cgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgagtttg ccaatatgat ccgcgatctc gatcaggaat gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg ggttgggcga aaactggcga ttcaaatcct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga
     4141 cgcatt
//
LOCUS       DAZKFU020000322         4141 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZKFU020000322 DAZKFU020000000
VERSION     DAZKFU020000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.324"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..3889)
                     /locus_tag="ACHOQQ_RS22330"
                     /old_locus_tag="pgaptmp_004466"
     CDS             complement(77..3889)
                     /locus_tag="ACHOQQ_RS22330"
                     /old_locus_tag="pgaptmp_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HGU4472626.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNSATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINAFGNIENRDGNSFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRFIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNTL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGLLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
BASE COUNT          980 a         1077 c          922 g         1162 t
ORIGIN      
        1 ctaaataaag acagcagcaa cccctgcgtg acatgacgca ttaatattaa aagccactgc
       61 tgtctttggt tacatcttac atgccgacaa tagtaccggt gttatcagtt tcgtttgctt
      121 ctaacgctaa accgtagaag ccaccgagaa cggcgtcttt accgctattc gtaaccttat
      181 ttgccgatac gccagcgaca ccataactca gcatattgag gtagttataa atattaccgg
      241 tggttttaac acccagagaa taatcaccca caatatttcc gcggttatta atgtcattca
      301 cggcattgat cgcgatggtt tgtcccaaca tattactgtt gctgttgttg gtaacatact
      361 tctgcgtatc aatagtcagc gcagtacccg ccacaatgtc tttaaagttc tcaacaccgt
      421 tcagggcatt aatggtcagc gcgttgtccg ccgcgatggc attacggtta tacagcgtgc
      481 ctttcagaac attgaaatgc acatctcctt taccgctgat ccaaccatag ttagtgacgc
      541 tgttatcaac gctcagatcc aggtttttgt cagacgcaat cacgcccgtg gcggtattgt
      601 cttccaggcg accatcgctg gcgttgttca acgacgccgc ataaacgtcg agattaccgg
      661 cgctgtacgt ggtggcatag ttgttgtaga acgtccctgc cgcacggatg atggcatcag
      721 ccccactgct aagaagcgcg cgggtattat ccagcgttcc cctggacagc agattaagcg
      781 gaccattttc agcgatgatg ctgctgttat tgttgtagac gttctgagca gaaagcgtga
      841 caccttcttt acctacgatg ccgccaacgt tgtcggtgat cccaaacagc gcgccgttat
      901 tacgcaggaa gttattcccg tcgcggtttt cgaggttgcc gccagcgtta ataaccagct
      961 ttttattcgc cgcgatcagc gcagtgttgt tgtaaatcgt cgatttagca tcgacagaaa
     1021 cattctcacc ggcaatcaaa ccataggcgt tgtctacgat gttcgccgtc agagtcacgt
     1081 cacccgtttc agaggtcagc aaacccttat agctgtcgta gttgcccgtc agtgccagcg
     1141 tgacatcgcc ataaccacga atttcaccgt tatcgttgtt cagattgttg cctttaacag
     1201 acaacacgcc gttcaacgca tgcagattac cgcggttgtt acccacgtta cccttcgaat
     1261 cgataatcag atcgcctgtc gcttcgatgg tcgcgttatt gttcgccacg ctatttgccg
     1321 acaatgcgat atccccatac cctaccatat agagatagtt atacaggttg cctttggtcg
     1381 tcacgttcaa gtcagcgtca ctgacaatgt tgccacggtt gttgatatcg ttaaccgcgt
     1441 taataacgat attctgcccc accatattgc tgttggagtt gttggtcaca tggcgattgg
     1501 tgttcatcgt caggtcgccg ccagcagaaa tatccttcca gttttcaata ccgttcagcg
     1561 ctgcaatgtc cagccccttt tcagccgcaa tagtattgcg gttgtacagg ttgccgttat
     1621 tcaccgtgac atcaacatca cctttaccgc tgatccagcc atagttggta agggtattca
     1681 ccacttccag tgacaggttt ttatcagatg ctatgaaccc ggtcgcattg ttatcgatca
     1741 tcgtaccgct actgctgttt tgcagtgtgg tcgcgtcgat atccaggtta cccgcgctcc
     1801 aggtggtagc gtagttgttg taatacgttc cgccaacctg aatagatgca tccgccccgc
     1861 tggtgaccag agcacgcgtg ttgtcgaacg tattctgtgc ctgcagagtc agaggaccat
     1921 cctcagcgat gaaacgactg ttgttgttat agatattctg cccggaaagc tcgatgcctt
     1981 ccttgccgac cattccaccc gtttgctgag gcatgccgaa gtacagacca tatgcattac
     2041 cgaagctatt gccatcgcgg ttttcaatat taccgaacgc gtttatcttc agttttttgc
     2101 tggccacgat cagcgcggtg ttgttattga cgccagacat cgactccatc gtgatgttct
     2161 gccccatcat gaagccgccg tggttgtcca ctgagttggc taacagggag atatcaccct
     2221 cttcagagct gatcacgcca atgttattgg tcaggctgcc gccaacggcg acttcaatgc
     2281 cctgcttacc gctgatcccc ccggtatcgt tacgtagtga gcttcccttg ataatcactt
     2341 tgctgttgga cagaattttg cccgcatagt cgtcgatcgt gctgtaactt gacagggaga
     2401 cgttgccatt agacgcaatc tgtccgccgt tgttattgat gttgttcgca ccaatttcta
     2461 cgccggtatc tgcaataatg cccagagagt cagaactgcc ggtatcggcg gttttggttg
     2521 aaccattatt tacgctacct gccgcgccaa taacgatatg cccggtggac gaacggatca
     2581 gacctttgtt gttatccaca ttaccgttac tgataatggc gatatcgccg gtggtctgga
     2641 tatcaccgtt gttgttattc agcgcagcgg attcaagacc cacatagcca ccgcgaatct
     2701 gaccattgct gttgttgagc gttccggttt tcagtgccac gagccccgct tcaatcccga
     2761 cagaactccc tttacctgag ttaatcagcg tggcgctatt ggtatcgacc gccagcatac
     2821 ctgctgcggc gagcttgccg ttggtgttat caatcgtgcc actgttaacg tagatatcac
     2881 ccatcgtaga gatgttaccc gcacgggtat tcacgatagt attcttgttg gtattgagcg
     2941 agatagtgcc tacagatgtc accgtaccgg tggtgttatc cagagtacca tttgttgtca
     3001 ggttgatcgt gcttgcagac tgaatctggg cgttactgtt taacaggtta cctttggaat
     3061 cgatgctgac accattaacc cccccagcaa taacgccgag gttgcgaacc ccaacacctt
     3121 tctcggtgct gaccagactg attttgttcg catacattcc gcccagtttg gcaacatcta
     3181 cgctgtaacc gttacgggaa cccgtggcgg atacgctacc ggtcacctgg cctgcggcat
     3241 taacatagtt attgccagca acaacgttca gctcatcggc agacactttg ccgttaacca
     3301 ccacgttacg ggacagaatt tctgtcgggc tggcgttatc cagtttaccg agcgtaatgg
     3361 taccgccgtt cacggagtaa ccggccagct tgtcatcctg gatatccggc gtcccggtgg
     3421 ttaaggtcag tttacctgta ttgattgaac cgccaccgtt tacagtaata ccattcgggt
     3481 tggcaataat cagatccgct ttatcccctg caacttccat catcccatta atggttgaag
     3541 gatttttgga agtaacctca ttcaggatca ccttcgccga cccggaggtc agattactgt
     3601 ttccctgaat atttccggca agtgaagtac tggattcatt agcgctgtta ttgaaaacga
     3661 caccattttt atcaacgttt aggttatccc agatattatg agataaaccg ctaccgttag
     3721 gtttgttgat gtcaacgact ggcacaccat tggcgttata taccgttcca ttttttatga
     3781 ctaatcccgc cgcttgagaa gctacaggaa aagacaacat cataagtata gcaatagggg
     3841 ttaatgcttt acccaccgtt gcttccttac ttaaatttat catcgccact aacgttttcc
     3901 ttattgatgc tattcatttt ggacatacag atcacttttg atgatttgcg gaatatcaac
     3961 aaaaatgatt gcggcaaatc ctatcacctg gcgcaataaa aaatcacgaa aaataagctg
     4021 aaataaggac tcaaaaaaca actaagagtt attgtttttt cttcgaacgg agacgtacct
     4081 aacttactaa tacacaataa tttaaaagtc cccacccctt ctccctgggt gagaaaaaat
     4141 c
//
LOCUS       DAZKFU020000323         4124 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZKFU020000323 DAZKFU020000000
VERSION     DAZKFU020000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.325"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            530..1087
                     /locus_tag="ACHOQQ_RS22335"
                     /old_locus_tag="pgaptmp_004467"
                     /pseudo
     CDS             530..1087
                     /locus_tag="ACHOQQ_RS22335"
                     /old_locus_tag="pgaptmp_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1227..2077)
                     /locus_tag="ACHOQQ_RS22340"
                     /old_locus_tag="pgaptmp_004468"
                     /pseudo
     CDS             complement(1227..2077)
                     /locus_tag="ACHOQQ_RS22340"
                     /old_locus_tag="pgaptmp_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2335..3204
                     /locus_tag="ACHOQQ_RS22345"
                     /old_locus_tag="pgaptmp_004469"
     CDS             2335..3204
                     /locus_tag="ACHOQQ_RS22345"
                     /old_locus_tag="pgaptmp_004469"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4472627.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLFHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(3364..3957)
                     /gene="rclC"
                     /locus_tag="ACHOQQ_RS22350"
                     /old_locus_tag="pgaptmp_004470"
     CDS             complement(3364..3957)
                     /gene="rclC"
                     /locus_tag="ACHOQQ_RS22350"
                     /old_locus_tag="pgaptmp_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGU4472628.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(3969..>4124)
                     /gene="rclB"
                     /locus_tag="ACHOQQ_RS22355"
                     /old_locus_tag="pgaptmp_004471"
     CDS             complement(3969..>4124)
                     /gene="rclB"
                     /locus_tag="ACHOQQ_RS22355"
                     /old_locus_tag="pgaptmp_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGU4472629.1"
                     /translation="LKHISVSSVSASPTVLEDAIADIARKYNASSWKVTSMRIDNNST
                     ATAVLYK"
BASE COUNT         1201 a          866 c          933 g         1124 t
ORIGIN      
        1 gagtaagaaa tataacttaa gtatctattt aatgacttgc acaaaaagct aaattttcac
       61 ccataaataa aaatataatc ccgcgcccaa ccacctgatg agtggctaca ggcactggat
      121 atattaggtg gcggtgtact ttcttacata aaggtatttc cttttctgcg gaaaaggaaa
      181 ccgggaaatc cccggttttt ctgacaagca gacgccatta tttgtgtctg cctatgttcg
      241 ttaattcgtt catcaggaaa ttatctcaat gtcacgttat aaaacaggtc ataaacaacc
      301 acgatttcgt tattcagttc tggcccgctg cgtggcgtgg gcaaatatct ctgttcaggt
      361 tctttttcca ctcgctgtca cctttacccc agtaatggcg gcacgtgcgc agcatgcggt
      421 tcagccacgg ttgagcatgg gaaatactac ggtaactgct gataataacg tggagaaaaa
      481 tgtcgcgtcg tttgccgcaa atgccgggac atttttaagc agtcagccag atagcgacgt
      541 gacgctgact gccagctcgg aaaatttagt tctggatcct aaagggacgg cgaaaactaa
      601 tgagcaagga caggctgttt tcaccggctc taccactatc gcagcgacat atacactcac
      661 ggcgaaagtg gaacaggcca acggtcaggt atcgacgaaa actgctgaat ctaaattcgt
      721 cgcggatgat aaaaacgcgg tgctcgccgc atctccagaa cgtgtagatt ctctggtggc
      781 ggacgggaag actactgcaa caatgacggt taccctgatg gcgggagtca atcccgtagg
      841 aggaagtatg tgggtcgaca ttgaggctcc ggaaggagtg acggagaagg attatcaatt
      901 cctgccgtcg aaggctgacc atttctcagg tgggaaaatc acgcgtacat ttagtaccag
      961 caagccaggt gtctatactt tcacattcaa cgcactgacg tatggcgggt acgaaatgac
     1021 gcctgtgaag gtgacaatta acgccgttgc tgcagagact gaaaatggcg aggaggagat
     1081 gccataattt gaatagtatg aaagttacga tctaataaag aataatggtg ttgaaatatt
     1141 caacgccatt attcactaaa taccttgcct gatactgatt aaatagtatc aggcaaggat
     1201 aaaactcaat aagagatagt gaccaactaa atcggacgaa cgggaataaa aatttcgcaa
     1261 acaacatcat tgccatcctt ttctacgaaa tcttcattgt aataaaagcg ctcaatatcg
     1321 ctgccgttca ctctggctaa ccccctggca ggcaactcta tcagatagat gttacgcacc
     1381 cagtcaggat aattttccca gtttccttca taacggaaag aggcataaag tcctcgggtc
     1441 gttgtccact cttttggcat gtcagcatat tcacataccg taccagcaaa agtttcaacc
     1501 gtgagcaaat cgaccgattt tgttgacggt tcaaacgtgg agacgaaata gatatccgtc
     1561 ttaaaattat tcatacatct ggctaaatga tgacgtcgca gtttgacttc ttcatccggt
     1621 gatttattcg acacttcaat atcatattta aatgaattgc caaaataggt gtctcaggaa
     1681 agttgactaa tctacactcc gtcttttgct gttgacgtat taaaaaagaa gggaagatat
     1741 ttgcgagatc ccaataatca cggtggcggt atacacgggg agagcagccg aataactttt
     1801 taaattcgcg gctgaatgac tgctgtgaat caaaatgcaa agagagtgca atatcaagca
     1861 tagatttcgc ggtgagccgg acaagaatgg cggcacgaca aagccttcgt ttgcgaatgt
     1921 attctcccaa aggcacatgc atgaaatttc ggaacagaag ctggatgttg cgtctgctgt
     1981 agccagattt ctgtgcgata tcttcgattg aaatagggtg ctcaagattg cactcaatcc
     2041 actccaggag ctgctgtaga atcttctgcc tgatcatcac attctccgtt aattctttgt
     2101 catatcgcac attttcgtta tgccctgggc cctaagtgta tcaaaaacag caagggcgaa
     2161 ggcaaaagca ctggtagcgt ctgcttcgtt gtccccgaaa atagagaggt ttcagtccta
     2221 cattattaat gaattttttg cataagtgat atcaaaatcc acatactaat ttgaggttac
     2281 gttttaacgt agactcattg ttcatgccta atgtgcctaa tggagggact gacagtggaa
     2341 ttttccgtat taagtaacaa tctgaaaatg ccgatgatgg gatttggtgt ttttcaggtt
     2401 accgataaaa acgtgtgcaa acagtcagtg ttgaacgcta tccgcacggg ttatcgactc
     2461 atcgacactg cagcggtata cggtaatgaa gatgctgtcg gtgaagccgt tcgtgaagcc
     2521 atatccgaag gtttatgtac ccgagaagag ttatttataa cgtctaaatt gtgggtgcag
     2581 gatatgttga atcaggatac ggcagcagca ggtattgaag catcattaaa aaaatcggga
     2641 ctagaatatt tcgacctcta tttatttcat caggctatgc gcgattattt cagtgcgtgg
     2701 cgtgcactcg aagatgccta tgaagagggc aaattaaaag cgattggggt ttccaatttc
     2761 tatcctcatg ttctggcgaa cttttgtgaa acggtaagag ttaaaccgat ggtcaaccag
     2821 gtcgagttgc atccctattt tgcccaacca gaggcgctgg caaccatgaa gtattataac
     2881 gtgcagcctg aagcatgggc tccgttaggt ggtggacgac ataaaccctt tgaaaataat
     2941 ctgcttcaga gtattgcaga tgcccatcaa aaatcgattt ctcaagtcat tctgcgttgg
     3001 aatattcaac ggggagtggt cgttattccg aaatcgacac atcaacagcg tatcgaagaa
     3061 aattttgcta tctgggattt ctcactgaca gagaaagaaa tggcacaaat tagttcgctt
     3121 gatttgggtt atgttgggga gtcggtaaaa cattttaatc ctgaatttgt tcgtggttgt
     3181 cttgctgtaa aaatacatga ttgatattaa tcatgtattt tacctgagac gacaagaatc
     3241 ttttaacagg ggagtgatat tgatcttcac tctgtcatat ctccggtaat atggcgtcag
     3301 gcttcatcat cagaaagggg gcctgacgcc tgaaaaagtg aacaacagac agtgttcgga
     3361 ttatcaatat tcagttttta acgttgaact ggattcatta ctgcgttgtt taagaatttc
     3421 ccgcgccgaa tctgccatta tcatgacagc acctgccagc atcagagtat ctttcaatac
     3481 caagcgacca gcaccggata aataagggaa accatgatga gcgtcaccca atgcgggtac
     3541 ccatgcctcc ggggtggtga ttaaaaatga gagtgttacc aacggtgtgg taaatgccat
     3601 cagccctccc aataaaccta accagcgatt gacaggatta gccaaaacca gcagagcaat
     3661 aatcacctcc acgacgccaa gaccgttgga aaaaccatag gtattattgg ccgattgcca
     3721 tgcccttgct tctggtttgt attcgccttc gtgagtcaga tactgtttat agtcttccgg
     3781 gtgttcataa aagaacgaca ttagtggact gtttgcgacg aatggtgtaa tgctgtctgc
     3841 ctcgtaaggg acaaacttta ataacccaat ccacataaaa acaatcgcaa tactcagacg
     3901 aatcaatgtc aggccaattt tatctccccg acttaacagg tgcaggtatt tttccattgt
     3961 gaacatcctt atttatacaa tactgctgtt gcggttgaat tattatcaat tcgcatcgat
     4021 gtgactttcc aggatgaagc attatatttt ctggctatat cagcaatggc atcctccaga
     4081 actgtcggtg atgctgatac tgacgagacg ctgatatgtt tcag
//
LOCUS       DAZKFU020000324         4118 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZKFU020000324 DAZKFU020000000
VERSION     DAZKFU020000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.326"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..785
                     /gene="artJ"
                     /locus_tag="ACHOQQ_RS22360"
                     /old_locus_tag="pgaptmp_004472"
     CDS             54..785
                     /gene="artJ"
                     /locus_tag="ACHOQQ_RS22360"
                     /old_locus_tag="pgaptmp_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGU4472630.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKISDQWFPQ"
     gene            complement(836..2317)
                     /locus_tag="ACHOQQ_RS22365"
                     /old_locus_tag="pgaptmp_004473"
     CDS             complement(836..2317)
                     /locus_tag="ACHOQQ_RS22365"
                     /old_locus_tag="pgaptmp_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HGU4472631.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWDPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(2329..2649)
                     /locus_tag="ACHOQQ_RS22370"
                     /old_locus_tag="pgaptmp_004474"
     CDS             complement(2329..2649)
                     /locus_tag="ACHOQQ_RS22370"
                     /old_locus_tag="pgaptmp_004474"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4472632.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            complement(2671..4014)
                     /locus_tag="ACHOQQ_RS22375"
                     /old_locus_tag="pgaptmp_004475"
     CDS             complement(2671..4014)
                     /locus_tag="ACHOQQ_RS22375"
                     /old_locus_tag="pgaptmp_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGU4472633.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
BASE COUNT         1183 a         1067 c          870 g          998 t
ORIGIN      
        1 ccatgccgca gacacggcca aaaatcataa gattgacaga cgggagttcc atcatgaaaa
       61 agttagttct tgccgcatta cttgcttcct ttactttcgg tgcttctgcc gcagagaaaa
      121 tcaattttgg cgtttcagcc acctatccac cctttgaatc tataggtgct aataatgaga
      181 ttgtcggctt tgatatcgac ctggcaaaag ccttgtgcaa acaaatgcag gcagaatgta
      241 cttttactaa tcacgctttc gacagcctga tcccgtcact gaaattcagg aaatatgacg
      301 ccgtaatctc cggtatggat atcacaccag atcgtagcaa gcaggtatcg ttcaccacgc
      361 cctattacga aaactcagcc gtcgtgattg ccaaaaaaga tacctacaaa acgtttgccg
      421 acctgaaagg caaacgcatt gggatggaaa acggtactac gcaccagaaa tatattcagg
      481 atcagcaccc ggaagtgaaa acggtctcct atgacagtta tcaaaatgcc tttatcgatc
      541 tgaaaaatgg ccgtattgat ggcgtatttg gtgacactgc ggtggttaat gaatggctga
      601 aaaccaatcc gcaactgggc gttgctactg aaaaagtgac cgatccgcaa tactttggca
      661 ccggtctggg tatcgctgta cgtccggata acaaagctct gctggaaaaa ctgaataacg
      721 cgctggcagc aattaaagct gacggtacat atcagaaaat cagcgatcag tggttcccgc
      781 aataattatt catttgccgg agtgggcgaa cacccactcc ggcaaaatca cttctctacg
      841 ctaatcccag gcgctgaaac tgctctggcg gcgcatcatt atctttcatc aactgaatca
      901 gcaacgcgat catccgggac tcgatcacat catcatgaat cggatgtagc tgctgcggat
      961 cgttttgcag atcaaacagc aaatgaccaa actgattcaa ccccttatat ttttcctgtg
     1021 ccgggatgcg taatacctgt aaccctttgg taaaacgaaa cggtggggcc agcgataaag
     1081 cccgcatctc atccaccgga tagcggctgt tcattttgca ggacatcaac gtgtagttgg
     1141 caatatcatc ctcacgcccc ggcagcgctg cacgcatata cacataacgc ccatcagtta
     1201 cgttagcatg accactgaaa acaccaaaca tcgccgagct gcgtactggt tcatcgtgac
     1261 tgaccgtttt cgccagcggt tgcccctgca catcgggcgg cactggctgt tgaaaaaagt
     1321 catacaacgt tggcgcccag tcgatcattt gtaccagtgc ctgtcgccgc tctcctttta
     1381 caccactgcg cgggtcccag ataaacaacg gattattggc aacttcgttg taataaggca
     1441 tctggttttt cgcccaccag ccatgctcac cgagtaaaaa gccgtggtca gtgccgacaa
     1501 tcagcatggt atctcgccac agatcgtgct catccatcag gtcgagaatg cgccccaggt
     1561 tgcgatcgca catcgacacc agcgaacgat accggcaacg gatgtgctcc acggcctcgt
     1621 cgctttcctg cacccggcca cgcggccagt cataatgcgg tccatcccag ttatcttcat
     1681 acaaggaaag atactcttcc gttgtgtgaa acggctcgtg cggatcaaac gtttcgatct
     1741 gcaataacca gttatcttcc gcgtgatttt tgtggataaa ggcacagcct gcatcaaaca
     1801 ctttagtttg tggaaaatcc gcttcctgct gaatatattc ccggtttgcc cagtcatggc
     1861 gccacagacc agaaacaccg ccaccggtct gcttttgcgg cacccgtagc acctcgggaa
     1921 taggcggttg cgcaacgctt gcatgccagt gatcgccttc ctgtccgcgc acaatctccc
     1981 acgaactgta gcgattatga taattcccgc cgccatcttc ccagtaatgc aaatgatcgc
     2041 ttatcaggtg ggtatagatc cccgcctttt taagcagctc cggcatggaa tcgtcaaacg
     2101 gttctaacgg cccccactcc cgatgcagga agttacatcg cccggtatgc aactcccgcc
     2161 gggcaggcat acacggcata ctgccgacgt aactgttttc aaaggtggcg gcatgagccg
     2221 ccagacgttg gaaattaggc gctttggtta acgcatcgcc ataaggtggc agataacgtt
     2281 tattcagact atcgaacagc agaataatcg ctctcattac gccctacctt attgattaat
     2341 taaatgcgga aaatgttgct gaaccacttc ataaatttgc tgaccaatag tttcaccgtc
     2401 cataatattc tctatcggaa tcaaatattg cccttgtttc gggtcaagac taaccacatc
     2461 gagtaatttt ttggaaatag caaaaatatc cgcccagtcg gtaaatccgc cgacttcacc
     2521 tgcggcggta tgttccattt caactttgaa atcatatttg gtcagcgctt ttttaacgac
     2581 cgtttgtaac ataacgctac tgcctacacc gtaaccacag acaaacagaa tattaattgt
     2641 atctttcatt tttaaaccct taatatttaa ttaacccgcg taaggaccaa gatggaatgt
     2701 tgcagcgata aatttcagaa cttcacaaat agcaggccat acggttgccc agtcaaatat
     2761 tcctgtccag ccgacgccat tttcagcaag cccggttaac ggaatagccc acaccgtacc
     2821 gaaggtctga ataataccca gcaagaacgt acagataatt acggaacggt atccgcccat
     2881 gcggtttgct aatacgccaa ttggaccgcc agagaaaaac agaggtacaa agccaggcaa
     2941 taccataatt gggctgtgaa ccagtaataa gatccccatc gaaaaaatgg tgccaaaagt
     3001 acagaagacg aagcctaacg ttgcggcatt gggtgaaaaa ggtaacagcg ctgcaacatc
     3061 gacagcagga atagcgttag gaataaagcg atcttgccag cctttaaacg aaccgttaat
     3121 ttcgcccacc atcatgcgca caccctgcag cagaatgacc atatacatgg agaactgtaa
     3181 gccaaggttg atgatataga tgtaccaggg cttgcctttc gccattaact ggacattatc
     3241 gatacccgtt gatagcaaga aaccaccgac aaacagcgtc atcacaatgg cgatagccgt
     3301 tacgttatgg ttaaaaatcg ccagccaccc gggaagtttc aggttttcag catcgtgctt
     3361 ttcagggtca ccaaatttat gggcgaattt ggagaaaaac cacaatccca acatctggtt
     3421 atgagcaatg gtaaaaccgg catctttggt aactttggca atgggcttaa caatgagcgt
     3481 ggtggagaac gcccagaata cacccgttaa tattccggca ataataatgg atgtagtcca
     3541 gctaaaaccc agccaaaaga caatcaacca taataccgcc tgagaatgtg aaacaccggt
     3601 attccctgtc aaaaatatcc ctttggcacc agtataccga ccaaataata ccagcagcag
     3661 gttggtaaaa aacgccagta caaatacata accgacataa ccaaaattat cccccaacgc
     3721 aatttgcgtt gccgctttca ttgcgtagga atccattaaa tatccgtgaa caccataggc
     3781 tttacttacg gcttctgcta ccggtttaaa tgttgcggaa aactggaaac caccaaataa
     3841 caccatcatt aatcccacca tggcgctgac catgccggta ataattttta ttggcgattt
     3901 cttatcaagg gcataaccaa tacccaccag caaaccaatt aaaattgccg gttcagttaa
     3961 aaacgtttga ataacatcta tgaatttggc aaacatcgtc ggaacgccct ccataagcgt
     4021 acatttccgt tgtcaatgca tgaaccttct gctgacgtta ataatgtctc aggaaatttt
     4081 gtgtttcgct aagcaatgcc tctgtatgta ttcggtaa
//
LOCUS       DAZKFU020000325         4114 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZKFU020000325 DAZKFU020000000
VERSION     DAZKFU020000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.327"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..117
                     /locus_tag="ACHOQQ_RS22380"
                     /old_locus_tag="pgaptmp_004476"
     CDS             <1..117
                     /locus_tag="ACHOQQ_RS22380"
                     /old_locus_tag="pgaptmp_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472634.1"
                     /translation="IEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPKAKA"
     gene            133..1470
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS22385"
                     /old_locus_tag="pgaptmp_004477"
     CDS             133..1470
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS22385"
                     /old_locus_tag="pgaptmp_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4472635.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSDRSLVDIVLA
                     LPLLNANGGNLDDGRESGRPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQKGVMWEGAL
                     HDARLREGADFWLSVRSSMPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            1467..2120
                     /gene="tssL"
                     /locus_tag="ACHOQQ_RS22390"
                     /old_locus_tag="pgaptmp_004478"
     CDS             1467..2120
                     /gene="tssL"
                     /locus_tag="ACHOQQ_RS22390"
                     /old_locus_tag="pgaptmp_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HGU4472636.1"
                     /translation="MNKPVISRAEQIFYPGWLMVCQLRSGQPVEDGKALYRRACQLVK
                     QAREELAEAGFSQENSDTMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIMTLAGLWCVLSSVLADQVARLTGQG"
     gene            2123..3823
                     /locus_tag="ACHOQQ_RS22395"
                     /old_locus_tag="pgaptmp_004479"
     CDS             2123..3823
                     /locus_tag="ACHOQQ_RS22395"
                     /old_locus_tag="pgaptmp_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4472637.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSRLLNDWQ
                     QHGVPQRYGLGLYQGMWLIPPVERATGEWVAPLPPRPVIQKVTQTPKTVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDW
                     MRDTGDIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEISLVPQVDACRLPDITRAP
                     "
     gene            3858..>4114
                     /gene="hcp"
                     /locus_tag="ACHOQQ_RS22400"
                     /old_locus_tag="pgaptmp_004480"
     CDS             3858..>4114
                     /gene="hcp"
                     /locus_tag="ACHOQQ_RS22400"
                     /old_locus_tag="pgaptmp_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HGU4472638.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSA"
BASE COUNT          813 a         1051 c         1272 g          978 t
ORIGIN      
        1 atcgaagaca acccgggatt tttccgcgtg aaactctatg ctgtaccaca tttccaggtg
       61 gaaggtatgg acgtcagtct gtcactggtt tcgcagatgc caaaggcaaa agcgtaaggc
      121 aggcgtcggg aaatgaagat ttatcgtcca ctgtgggaag acggggcttt tctgatgccc
      181 cagcagtttc agcagcaggc tgcctgggat gttcatctgg cagacagcgt tgcccgaatg
      241 gggctggcgc atccctgggg cgtggtggct gcagagtttg atgactctct gttaccgctt
      301 tcacggttga atgccacccg tctgattgtt cgtttcccgg acggaacgct tattgatacg
      361 gaacgtgcgg ataatctgcc tccggtctgt gatttatcga cggtttctga tcgttccctg
      421 gtggatattg tgctggcgct accgttactg aatgctaacg gtggcaacct tgatgatggc
      481 cgtgagagcg ggcgtccgcg tcgctggaaa tcagaaaggg tgaatgtgca ggagctggcc
      541 gggcatgagc aaagcgaagt ggcggtattg cggcataacc tgactctgcg catggctcat
      601 caggaaaatg ctgcctggct gacctgtccc gttacccgtc ttgttcgtga tgcacaggga
      661 cagtggtgca gggacccgcg ttttattccc cctctgctga cgctgtctgc cagtccttcg
      721 ctgatgactg agcttgcgga gttgcttcat cacctgcagg cgcgtcgcca gcgactgatg
      781 tccatgcgtc gggagaacaa tgcccgtctt gcggattttg cagtggcgga cgtgtcgttg
      841 ttctggttgc tgaatgccct gaacagtgcg gagcctgttc tgaaagagtt gcttgacatg
      901 ccttaccgcc acccggaact gttgtacagg gagcttgccc ggctggcagg ctccctgctc
      961 acattctcgc ttgagcataa tgtggatgcg gttccggcat accgtcatga aacacctgaa
     1021 aatgttttcc cacctttgct ttctttgctg aaccagctgc tggaggcaag cctgccttcc
     1081 cgtgtggtct tcattgaact gaaacaaaaa ggtgtgatgt gggagggggc attacacgat
     1141 gcgcgcctgc gcgaaggagc ggatttctgg ctttcagtgc gctcttccat gcccgggcat
     1201 gaactgcaga cgaagtttcc gcagctatgc aaagccggga gccccgatga tgtgtctgag
     1261 gtggtgaatg tggcgctgag cggggtcatt atccgtccgg ttacgcatgt accggcggcc
     1321 attccgctcc ggctggaaaa ccagtatttc gcgctggatt taagtacgga tgcggcccgg
     1381 gcgatgctgg acgcggggcg ttgtactttc tacaccccgg catcgctggg agatgtgaaa
     1441 ctggaacttt ttgcggtgct gcggacatga ataaaccagt tatttcccgg gctgaacaga
     1501 ttttttatcc cggctggctg atggtctgcc agctgcgcag cggacaaccg gttgaggatg
     1561 gtaaggccct gtatcgccgt gcctgtcagc tggtgaaaca ggcgcgtgaa gagctggccg
     1621 aagcaggatt cagtcaggaa aacagtgaca ccatgttgta tgccttctgt gccctgctgg
     1681 acgagagtgt gctgaaccgc gaaaaaacag acgatggctg gcacacctgg cagcaggacc
     1741 cgctgcaggc tcattttttt ggtacgctca atgccggtga agagctctgg gagcgtattc
     1801 gtgagcaact gaaattacca gcgccggatg tggcggtact gacatgcctc tgccgtacgc
     1861 ttcagctcgg ttttaccggt cagtaccggt cgcaggatga tgagcgtcgc gaagatgtaa
     1921 tacgcgcact gacagcgcgt gttcctgcat ttactttcgc acaggatgcg ccggtggtgg
     1981 tgcgtgcacc ggggtaccgt gctggcagaa cgatgtactg gttgtcatgg gtggcgggta
     2041 ttatgacgct ggctggtctc tggtgtgtgc tttcttctgt gctcgcagac caggtggcac
     2101 ggcttaccgg gcagggataa ccatgcacgt acgttcccgt ctctggctga caatactgag
     2161 tctgttcctg acactgtggc tgatttcggg attcctgcca ctgtctgcag taaaccagat
     2221 aatactgacc ctggtttcag ttgtggcggc ggggcttgtg ctgtggtatc agtggcgttg
     2281 tctgtcttcc cgtcgtgata tcccggagaa tgccggcgat gacggtctgc cgcctgagta
     2341 ttttcagggg catgtgttgc ttgttgccgg aaactcagat gcgtggttcg aacccggtca
     2401 ggcataccgg gaaacagcat cgggatggta tcttcgtgtg gatacgccgg agcagatgag
     2461 aatactggca gagcggttgt cgtacagccg tccggccctg tgtggccagg tcgtggtcat
     2521 gctgggactt ttaccggagc agcatacctc agatgaagct ttcctgtcgt ggttgcggaa
     2581 ctggcagcgt gcagtcatgc agtgccggca gtggctgaat accatgccgc cggtgctggt
     2641 gacattgatg gtttcagggc cgtcaccaca aaacacatcc ttaaataagc actctgcaca
     2701 ctggtttaca ctgacaccgg acctgtcggg aatgcacata cgcctgacgg aagccggagc
     2761 cattccggca gaggaatggc tccgggagtg tgatgcagcg tccaggctcc ggtgcgtcag
     2821 tgaactgtta tggctgaatg ccatgttgtc ctggtacaac cgggttactg atgccctgac
     2881 agatatttgc cggcaggagc acttttattt ccggccggtt gccgttggat tctgtctgac
     2941 gtcggttgcg gcccggcccg gtaatctctg gcaacaacaa ctgacgtcac tcactgtact
     3001 gccaccgcct gccggcgtgt catcacaggc actgccgttg ccggatattc tgctgtccgg
     3061 tattccccgt catcgtggac tcagtcactg gcacagactt tgtctccaga cgggggcgat
     3121 tgtcggcgtt tttcttctgc tggctatgct ggcgtctttt atgaataacc agcgactggt
     3181 tcgcagcgtg ggggaacacc tgacggcctg gcaccggatg cgcggatatt catctgaatc
     3241 gaaaaaaact gcactgcaac gtcttcaggc tgacagccgg ttactgaacg actggcagca
     3301 acatggtgtg ccgcagcgtt acggactggg attgtatcag ggaatgtggc tgataccgcc
     3361 ggttgagcga gcgactggtg agtgggttgc accactgcct ccccgtccgg tgatacagaa
     3421 agttactcag acgccgaaaa cggttcgcct taacagcctg gcgctgtttg atgccggtaa
     3481 atggacgctg aaaccgggcg ccacaaaatg gctggtgaat gccctggtgg atattaaggc
     3541 aaaagcgggc tggctgattg tggtcagtgg tcacacggac aatacgggtg acccgcagcg
     3601 caaccaggcc ctgtcactga agcgcgcaga ggccgttcgc gactggatgc gggatacggg
     3661 cgatatccct caaagctgtt ttgccgtaca gggatatggt gagagtcgtc ccgtggctcc
     3721 gaatgagacg gcggaagggc gagcgcgtaa ccgccgtgtt gaaatcagtc tggtaccgca
     3781 ggtcgatgcc tgtcgtctgc cggacatcac ccgggcgccg taacagggcg cgtaagacaa
     3841 cgagagggag taactttatg gcaattcctg tttatctgtg gctgcaggat gatggcggag
     3901 cggatattaa aggctccgtg gatgtgaagg accgtgaggg cagtattgaa gtggtggctc
     3961 aggagcactg cctttacatt ccgacggata acaataccgg taagctgacc ggtacccgta
     4021 ttcacacgcc gttcctgttt accaaggaaa ttgattcttc cagcccctac ctttacaagg
     4081 cggtgaccac ggggcagacc ctgaagtctg ctga
//
LOCUS       DAZKFU020000326         4104 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZKFU020000326 DAZKFU020000000
VERSION     DAZKFU020000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.328"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            164..724
                     /locus_tag="ACHOQQ_RS22405"
                     /old_locus_tag="pgaptmp_004481"
     CDS             164..724
                     /locus_tag="ACHOQQ_RS22405"
                     /old_locus_tag="pgaptmp_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472639.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQRLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            711..1295
                     /locus_tag="ACHOQQ_RS22410"
                     /old_locus_tag="pgaptmp_004482"
     CDS             711..1295
                     /locus_tag="ACHOQQ_RS22410"
                     /old_locus_tag="pgaptmp_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472640.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            1319..2185
                     /locus_tag="ACHOQQ_RS22415"
                     /old_locus_tag="pgaptmp_004483"
     CDS             1319..2185
                     /locus_tag="ACHOQQ_RS22415"
                     /old_locus_tag="pgaptmp_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU4472641.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            2178..2540
                     /locus_tag="ACHOQQ_RS22420"
                     /old_locus_tag="pgaptmp_004484"
     CDS             2178..2540
                     /locus_tag="ACHOQQ_RS22420"
                     /old_locus_tag="pgaptmp_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HGU4472642.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYENLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            2557..2781
                     /locus_tag="ACHOQQ_RS22425"
                     /old_locus_tag="pgaptmp_004485"
     CDS             2557..2781
                     /locus_tag="ACHOQQ_RS22425"
                     /old_locus_tag="pgaptmp_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472643.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            2778..3680
                     /locus_tag="ACHOQQ_RS22430"
                     /old_locus_tag="pgaptmp_004486"
     CDS             2778..3680
                     /locus_tag="ACHOQQ_RS22430"
                     /old_locus_tag="pgaptmp_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472644.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            3673..3915
                     /locus_tag="ACHOQQ_RS22435"
                     /old_locus_tag="pgaptmp_004487"
     CDS             3673..3915
                     /locus_tag="ACHOQQ_RS22435"
                     /old_locus_tag="pgaptmp_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472645.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKAWAPAQPDELA"
     gene            3936..4082
                     /locus_tag="ACHOQQ_RS22440"
                     /old_locus_tag="pgaptmp_004488"
     CDS             3936..4082
                     /locus_tag="ACHOQQ_RS22440"
                     /old_locus_tag="pgaptmp_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472646.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
BASE COUNT         1245 a          957 c         1013 g          889 t
ORIGIN      
        1 agaacagtaa ttaatttaca gctttattgt cggggatttt tacgacctga aaaggccagg
       61 ctgtatcaca gatatatcgc aacaaacgaa ttgctgagcc acatcttctg gctgcgctca
      121 gagtgaataa aagtaattaa caccggatga acacggagta accatgaaag aagaacctga
      181 aatcattgaa attaattctg aagaattcac agaaaacgaa tggctgacag aaaccgatcc
      241 gcgcgttctg gcgctgacaa tggaaggata catcgtattt ccacctttct tcagtgatgc
      301 ggaatacagt gcaataaata atgccggaat cgaccgtgct tttcataaat acgaactcga
      361 ttgcggccct gtttacagag tctgccgcca cggacaagtt aagaaatgct tcacgcgaaa
      421 aacggccatc atacatctcg ctcattttat gacaacgaag gtattcgaaa gttcaggttt
      481 tgaaaaacgt ctgcctgacc agcgcctgat gcatccggaa tatggagaag tctttcatcc
      541 gggagaaatt acgcccgaat actacagagc tcacgggcgt tgttttcatc gcctgttgcg
      601 catccttgca cgcagaaaac agtttgatgc ctggatggaa aaatacaacg cctggattga
      661 tcagtattgc cggcttgtcg cgcaaagacc atttaagaag agagcaaacc atgactgcaa
      721 ctgaacagtt attttttctt accgctgaag gtatccacac accttacgga ctgcttgata
      781 tcaacaaagc actggaaatg ctcgatgaag gctgttttga cgagccgatc tggcatggtc
      841 tggaaatagt gtgttgcatc agtgaagcag aagaaaaagg catgacaggt gagctagcgc
      901 cggaaaagac aatcatgtac tggcgctggc tttataccgc catgttcttc tctgaacagc
      961 tcaccagaaa cggctggacc gaagtgctta acgagaatgg cggtacagac aagggcacca
     1021 tgtacagagg ccacaacggt ggcatgacaa tctacctgaa accattacgt ctcgctatgc
     1081 agaactatat ggaatgggca ctcatagaaa cttacggagc ggaatcaggg ctgctcaaaa
     1141 gcattcttat gtacaaagca ttcaccggcg ctctgggcga cggcgaaaaa accggcatca
     1201 caatggaagg ttacagagcg atgcacatga ttctggatgg gcttatcagt gaagccactg
     1261 acaactcaga accggaaacc agagtgttcc actaatcagc ttttcgtgag gtgtaatgat
     1321 gaaaaaatca actgaacttg tgccggtggc agcccgtgat ttgcaaatta tcgaatatcg
     1381 cggtcaacgc gttgtgacca ctgaacaact ggcggcaggt tatggagcaa caccaatccg
     1441 tattcgccag aatcattccc gaaacgaaga acgattcgtt gttggaaagc attatttttt
     1501 gctggaaggc gatgaactac gtgaacttaa gcacagagta tctcaaagct actctgtgaa
     1561 aattgccagc aatgttcgat ccctcatcct ctggaccgaa cgcggcgcag ccaaccacgc
     1621 aaaattgctg gaaacggatc aggcgtggaa ctatcacgaa gaccttgtgg agttctactt
     1681 cactcagcgg gaaaacatcg atgttcagcg cgtactcact cgcaaggaac tcgcgttgat
     1741 ggttattgag gccgaagaac gggctgaagc tatggccctg gaaaataaaa cgctctctgc
     1801 aaccgtagac agtctggaaa aacatttctc taaaggcatg actgttcccc agttctgccg
     1861 tgcgctgaac ggcgtaaata ccagccagat aatgtggtgg ctgtctggcc gaaaatgggt
     1921 attcaacgaa caacgcgatc cggagaaaac accccgctgg cgcacggcct cttatgcccg
     1981 tgacagatac ctgaccgaag aagcagtaca gatcactcca cacgggaaag caacattcac
     2041 gaaattcacc cccgtattgc tggaaaaagg atgccaccgt ttataccagt tttacatgaa
     2101 cggtgaactc cctatgaaga aaaactggaa cggtgaattt aaacacgaca aagctattta
     2161 cacaccggag gcagaacatg aatagcacta ctgcggcaat catgaactac gacccgaaca
     2221 tgacattatg cgggcgcatg gcaagacaga cggttcgtct gacactcggg caatgggagt
     2281 accgcgaaac atttgaagtg gacgttgtcg gcaatctgac gggactggat gtaataacct
     2341 gcgcaattga aaacctttat gaaaacctgc cgtatgaaga agcacaggat gaatataccg
     2401 gtgaaactaa cattatggcc acaatacaga tcggtgatct tgcatgtacg gatgaggaca
     2461 tgctcggcga agcatggctg gcaggtatgc ttattgcagc agaaattatc agcattgaac
     2521 cagcaggaac actttcataa cggagatcgc catcagatga ttatttcatt caccgattac
     2581 ggcgaacagg caaaactgat tatcaccagt tcccgcctga cattgcgaaa acataatcgt
     2641 gtcgttgatg cggtacttct gtgcacgccg gacatcacag aaagccggag cggctttttc
     2701 ctggttaagt cgtcattatc aggaaaaaca cgcgacatcc tgcgcgccta caaaacagca
     2761 acaagggagt ctgaacgatg aaagaagctg atctgaaagc actgcgtgac gccgcacaaa
     2821 ataccgtata tggagacaag ggatgtgatc tggaagcatt tcactccctt gccactccgc
     2881 cagttatcct tgcgctaatc agtcacatag aaaatctgga gaaaaaactg gatgaaatcg
     2941 taacgtcaga gattagcccg aaggcctggc tgaataacct tacgggggaa gtttgcagta
     3001 ttgatgccat gcctgacgcc cgtaatgaca gagaaacata caccccgctt tatcctgcct
     3061 ggcagcgcat tgccgccgca gatgataacg gcagagtggc atggatgtat acgcacaaaa
     3121 aagcaggtta cagggtcccc acaatgacat cgtgggaaag tcttgcagaa gagcggggag
     3181 gctctctgga taaatatatc gtcagtccac tattcactgt tcctcagata ccgccagtga
     3241 gaacagaaca ggatgcttac cctgatgtgc aggtttcatg ggatgcaaca cgtcactatg
     3301 tcaacggctg gaacgcctgc cgacaggcaa tgctgacgac ggcagaaaaa gtacttgatg
     3361 tgcgaatccc tgaatatatc ggacgctggc tttccgctgc acttcatgat cagaactcat
     3421 gcgaggaaat gaaaaacgat atacgacgct tcctggctga ataccaccat gttccatcga
     3481 aatataacgg ctggcgactt gtaccggaca agttaaccga acagatgaag taccggattg
     3541 tgccactact ggcgaccatc aagccccgca gcgtcgatga atatgaccgc tcaggagaaa
     3601 acgcacaatt tcttgaatgg gtatggacgc aactgatgga agccacgaaa ttcgcgtatg
     3661 agggctcagt gaatgggtga aatggttgat attaacgatg aactggtccg cacagtaatt
     3721 accgttgatg acggatgcga ccatacccag cgttttatct ggatgatgaa tacacggcgt
     3781 aatatcagaa acaaagtgtt ccggcacgaa ccaaagccag tcagggtgac aaagatacgc
     3841 attgaagcca gaaaacgccg caaatacagg ccacggaagg cctgggcccc agcgcaaccg
     3901 gatgaactgg cataaagaca gcaggagaat aagtcgtgaa tcgcgagttt gaaatatgga
     3961 tacgactcag atacgggtac cgatacgatt taaccagaga tgctcacggc ttctactgcc
     4021 gtgaagttgt aaagcgaatg tttgacgtgt ggtgccactg ccgtggcctg aagatagtgt
     4081 gagtcaggaa tggaaacaat catc
//
LOCUS       DAZKFU020000327         4099 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZKFU020000327 DAZKFU020000000
VERSION     DAZKFU020000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.329"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(66..758)
                     /locus_tag="ACHOQQ_RS22445"
                     /old_locus_tag="pgaptmp_004489"
     CDS             complement(66..758)
                     /locus_tag="ACHOQQ_RS22445"
                     /old_locus_tag="pgaptmp_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4472647.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQLGKPTANHPKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            869..1096
                     /locus_tag="ACHOQQ_RS22450"
                     /old_locus_tag="pgaptmp_004490"
     CDS             869..1096
                     /locus_tag="ACHOQQ_RS22450"
                     /old_locus_tag="pgaptmp_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4472648.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            1127..1666
                     /locus_tag="ACHOQQ_RS22455"
                     /old_locus_tag="pgaptmp_004491"
     CDS             1127..1666
                     /locus_tag="ACHOQQ_RS22455"
                     /old_locus_tag="pgaptmp_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HGU4472649.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQAPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            1663..2682
                     /locus_tag="ACHOQQ_RS22460"
                     /old_locus_tag="pgaptmp_004492"
     CDS             1663..2682
                     /locus_tag="ACHOQQ_RS22460"
                     /old_locus_tag="pgaptmp_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4472650.1"
                     /translation="MTGSEMAKAGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     VAQKERRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNIGINNVISEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSDESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            2679..3380
                     /locus_tag="ACHOQQ_RS22465"
                     /old_locus_tag="pgaptmp_004493"
     CDS             2679..3380
                     /locus_tag="ACHOQQ_RS22465"
                     /old_locus_tag="pgaptmp_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4472651.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGIATMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGARV"
     gene            3377..3679
                     /locus_tag="ACHOQQ_RS22470"
                     /old_locus_tag="pgaptmp_004494"
     CDS             3377..3679
                     /locus_tag="ACHOQQ_RS22470"
                     /old_locus_tag="pgaptmp_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HGU4472652.1"
                     /translation="MTGKEAIIHYLGTHKKFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            3747..4079
                     /gene="emrE"
                     /locus_tag="ACHOQQ_RS22475"
                     /old_locus_tag="pgaptmp_004495"
     CDS             3747..4079
                     /gene="emrE"
                     /locus_tag="ACHOQQ_RS22475"
                     /old_locus_tag="pgaptmp_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HGU4472653.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
BASE COUNT         1126 a          929 c         1036 g         1008 t
ORIGIN      
        1 aaaatgaata attaagttgt tttactgaat gagcacaatg cccggttgat aatttttaat
       61 tggtactatc catgcttcct atacgtctgc ggcatgctcc caataacttt cccgaagata
      121 aatacccggt tcatctcatc tttctcgatc gggtcccacg gtgagtagct tttgttatca
      181 gagataacca gcagcttatc cttcatcatt tgcaggcgct ttacatgggc tgtgtcgtcg
      241 tacagaaacg catagatacc atcaccgtcg aaagatttaa ccgtgatatc aacgaataga
      301 agatcaccag gttcaatcgt tcctgacatg ctgtcaccgc gtacgttaat gatgcggata
      361 ttttctgcct tcctaccatc gaacatgtga cgagcatcgt caaacgagta ctcaaccgag
      421 cgtagaactt ctacaaactc acggttgatg actcccggcc cggcactcac ttctatatca
      481 agaacgtcaa tcttgaagta tttgggatgg ttggcagtag gcttccctaa ttgttgaccg
      541 tcatttctca tcgggcctat gcctgatgag agccactctg ttcgaacacc caatgcatta
      601 gctatttcaa caatttttgt tgagccgcgc gcgttgccgc ttgtcagtct ccagattgtg
      661 ggttgagcta cgccagacgc ctttgcaaga gcgccttgag acattccaga ttgttccatc
      721 gctaggttta agcgatcagc aagagtttct tttttcataa gttttaattt atacgcttgc
      781 gtattgatgg tcaaaacacg ttttgctatt gttatgatta atacgtattg ctattattta
      841 ttcattgtaa taccaatagg aattgataat gacaaatcaa accattcaac tcgcaatcag
      901 tattacaggt agtcaaaaac gactggcaga tctatgcggt gtagcccagc ccactgtttg
      961 gcgttggcta cacggtggcg gaattgatgc ccgttatgta atgaaaattg tctcagccac
     1021 tggtggaaag attaaaccag cagatattcg tcccgacctc gcaccattgt ttaacgcgag
     1081 taattctgcc gcctaatctg cggcgttaac tgataaggca atgactatgc aaccacttac
     1141 ataccaacag actagcggat ttagcccgac tgcggtgata aatcgttctc aaacaaaaca
     1201 agctccaggc cacgaaaaaa tccgtgatgc cgtccgcgcc tggtcggctg cagataatca
     1261 ggatgttgtt gccgcactca ttgtgaatga gtatcgggag cagggcggcg gcaccatcga
     1321 tttccctgat gatgtcagcc gtgcacgcca gaagctgttc cgcttcctcg ataacaaatt
     1381 cgattctgaa aaataccgaa ataacgtgcg tgaactgacc ccggcaattc tggcggtact
     1441 accgctggaa tatcgcggcc acctggttga gcaggatagc ttcatggctc ggctggctga
     1501 aatggaaaag gaactcagtg aggcaaaaca ggctgtcatt ctcaacgcac cacgccacca
     1561 gaaactgaag gagatgagtg aaggcattgt gtcgatgttt cgtgtggacc cggatctggc
     1621 tggtccactg atggcgatgg tcaccaccat gctgggggca atatgacagg ttcggaaatg
     1681 gcgaaagccg gtctgcgcga acagaaccga ctttcaggtg caaatcgtaa cacactcatt
     1741 gcgggaggaa ttatggcaaa cactgctgag atattcaatt ttccagtgcc ggatgtggca
     1801 caaaaggagc ggcgcgtggc agatctcgat gatggttata cgcgcattgc aaatgagttg
     1861 ctggaagctg tgatgctggc cggattaaca cagcaccagc ttctggtctt tctggctgtc
     1921 atgcgcaaaa catatggctt taataaaaaa ctggattggg ttagcaacga gcaactttcc
     1981 gaattaaccg ggatattgcc gcacaagtgt tctgctgcaa aaagtgttct ggtaaagcgt
     2041 gggattttga ttcagagcgg gcggaatata ggcattaata atgtgatcag tgaatggtca
     2101 acattacccg aatcaggtaa gaaaaataaa gtttacctga aagaggtaaa tttacctgaa
     2161 tcaggtaaga aaagtttacc caaatcaggt aaaggcgttt acccgaatca ggtaaacaca
     2221 aaagacaaac taacaaaaga caatataaaa cctttttcgt ccgagaattc tgacgaatcc
     2281 tctgaccagc cagaaaacga tcttcctgtg gtgaaaccgg atgctgcaat tcagagcggc
     2341 agcaagtggg ggacagcaga agacctgacc gccgcagagt ggatgtttga catggtgaag
     2401 accatcgcgc catcagccag aaaaccgaat tttgcagggt gggctaacga tatccgcctg
     2461 atgcgtgaac gtgatggacg taaccaccgc gacatgtgcg tgctgttccg ctgggcatgc
     2521 caggacaact tctggtccgg taacgtgcta agtccggcca aactccgcga caagtggacc
     2581 cagctcgaaa tcaaccgtaa caagcaacag gcaggcgtga cagccagcaa accaaaactc
     2641 gacctgacaa acacagactg gatttacggg gttgatctat gaaaaacatc gccgcacaga
     2701 tgattaactt tgaccgtgag cagatgcgtc ggatcgccaa caacatgccg gaacagtacg
     2761 acgaaaagcc gcaggtacag caggtagcgc agattatcaa tggtgtgttc agccagttac
     2821 tggcaacttt cccggcgagc ctggctaacc gggaccagaa cgaactgaac gaaatccgcc
     2881 gccagtgggt gctggctttc cgggaaaacg ggatcgccac aatggaacag gttaacgcag
     2941 gaatgcgcgt agcccgtcgg cagaatcgac cattcctgcc atcacccggg cagtttgtcg
     3001 cctggtgccg ggaagaagca tccgttaacg ccgggctgcc aaacgtcagc gagctggttg
     3061 atatggttta cgagtattgc cggaagcgtg gcctgtatcc ggatgcagag tcttatccgt
     3121 ggaaatcgaa cgcgcattac tggttggtta ccaacttgta ccagaacatg cgggccaatg
     3181 cgttgactga cgcggaatta cggcgcaagg ctgccgatga actgtcctgt atgaccgcac
     3241 gaattaaccg tggtgaggct atacctgaac cagtaaaaca acttcctgtc atgggcggta
     3301 gacctctaaa tcgtgcacag gctctggcga agatcgcaga aatcaaagct aagtttgggc
     3361 tgaaaggagc aagggtatga cgggcaaaga ggcaattatt cattacctgg ggactcataa
     3421 gaaattctgt gcgcaggacg ttgccgcggt aacaggcgca acggtaacca gcataaatca
     3481 ggctgcggct aaaatggcgc gggcaggaat cctggtcgtt gatggaaagg tctggcgaac
     3541 ggtgtattac cggttcgcta ccagagaaga acgggaagga aaggtgagca cgaatctgat
     3601 ttttaaggag tgtcgccaga gtgccgcgat gaaacgggta ttgagggtat ataaaagaac
     3661 atcaatgggt acacaatgat gaaacaggtg agttgagttc aaactgtagt acaattctct
     3721 ccagtttgaa caggaaagaa tatgctatga acccttatat ttatcttggt ggtgcaatac
     3781 ttgcagaggt cattggtaca accttaatga agttttcaga aggttttaca cggttatggc
     3841 catctgttgg tacaattatt tgttattgtg catcattctg gttattagct cagacgctgg
     3901 cttatattcc tacagggatt gcttatgcta tctggtcagg agtcggtatt gtcctgatta
     3961 gcttactgtc atgggggttt ttcggccaac ggctggacct gccagccatt ataggcatga
     4021 tgttgatttg tgccggtgtg ttggttatta atttattgtc acgaagcaca ccacattaaa
     4081 agtaatttgt ttctaaacg
//
LOCUS       DAZKFU020000328         4070 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZKFU020000328 DAZKFU020000000
VERSION     DAZKFU020000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.330"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..199)
                     /locus_tag="ACHOQQ_RS22480"
                     /old_locus_tag="pgaptmp_004496"
     CDS             complement(<1..199)
                     /locus_tag="ACHOQQ_RS22480"
                     /old_locus_tag="pgaptmp_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4472654.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLC"
     gene            complement(501..794)
                     /gene="yicS"
                     /locus_tag="ACHOQQ_RS22485"
                     /old_locus_tag="pgaptmp_004497"
     CDS             complement(501..794)
                     /gene="yicS"
                     /locus_tag="ACHOQQ_RS22485"
                     /old_locus_tag="pgaptmp_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGU4472655.1"
                     /translation="MKLTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAIERNNQNNYWEALGKVECPDM"
     gene            1003..1821
                     /gene="nlpA"
                     /locus_tag="ACHOQQ_RS22490"
                     /old_locus_tag="pgaptmp_004498"
     CDS             1003..1821
                     /gene="nlpA"
                     /locus_tag="ACHOQQ_RS22490"
                     /old_locus_tag="pgaptmp_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGU4472656.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            complement(1825..2748)
                     /locus_tag="ACHOQQ_RS22495"
                     /old_locus_tag="pgaptmp_004499"
     CDS             complement(1825..2748)
                     /locus_tag="ACHOQQ_RS22495"
                     /old_locus_tag="pgaptmp_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGU4472657.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPSALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(2859..4043)
                     /locus_tag="ACHOQQ_RS22500"
                     /old_locus_tag="pgaptmp_004500"
     CDS             complement(2859..4043)
                     /locus_tag="ACHOQQ_RS22500"
                     /old_locus_tag="pgaptmp_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HGU4472658.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARNSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNVPVVTQPVEILPSIHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1191 a          913 c          933 g         1033 t
ORIGIN      
        1 tacagagaaa tttaaaaaag agttcagctt cgtcaagtct tcctttttca taaaagttat
       61 aagcgtaagc ataaacgcct tcaagggtat ccggagtaat gccatgcagc tctgcaagtg
      121 ttgctcccat acctagtgca cgttcgaact tactggcaat ttctttattt tcttctaaat
      181 ccagatttgc gctttccatc tctactctct cctttcgaac attctttgct cagacttcac
      241 cattttttag ggggtggtga tatcaaaatt cgctcatttt tatgcccatc cgttgggctt
      301 cgttttctgc attaaaaaca agtattgttt caatgagata agattttcta tgtgatgaaa
      361 tgaaaataat gtagagggaa atttatccac aatcctgatg aactggttca attttgattg
      421 gaatttgaaa aagcaggtga tttactgatg tgcgagctta agtcagcggc tgctggcctg
      481 agcgcggagg tgtgcgaaat tcacatatcc gggcattcta ccttacccag cgcttcccag
      541 tagttattct gattattacg ctctattgcc acaatagctt cgcggatatg ttgtttgtta
      601 ctctcatcag acaacattaa tttttcccac gcttcgtctg ataatgacgc ctgtggcgtg
      661 caaattttac gtaaatcttg caataccgtg gttttctctt ttgccgactg tagcgaactg
      721 aatggcgact cggcctgaga aatggctgga aaagtcagga atacggtaat catgagtagc
      781 atcgttagct tcatagcggc aaccttctgc aaatgtgcat cgaatgaagc ataagtgtag
      841 tttgcttttg ccgctctcat catgctgtac gaaaggtgtt tgcgtggtca gtaagaagtg
      901 ccagtttttt ataccttttt cttctattcg atagcttttc gttttttaaa tcgtagtgat
      961 cttgccatta tagtcagtta atgattaaaa aaaggataaa aaatgaaact gacaacacat
     1021 catctacggg cgggggccgc attattgctg gcaggaattc tgctggcagg ttgcgaccag
     1081 agcagcagcg atgcaaaaca cattaaagtt ggcgttataa atggcgcaga acaagatgtc
     1141 gcggaagtcg ctaaaaaggt ggcaaaagag aaatatggcc tcgatgttga actggtaggt
     1201 tttagcggtt cgttgctacc gaacgatgcg acaaatcatg gcgaacttga tgcgaacgtc
     1261 ttccagcatc gaccctttct tgaacaagat aatcaggcgc atggttataa actggtagcg
     1321 gtgggaaata cgtttgtctt ccctatggcg ggttattcca aaaaaatcaa aaccgtggcg
     1381 caaataaaag aaggcgcgac agtggcgatc cccaacgacc cgaccaacct tggtcgcgca
     1441 cttttactgt tgcaaaaaga gaagctaatt actctgaaag agggaaaagg gttattacct
     1501 accgcgctgg atataaccga taatcctcgt catttgcaga ttatggaact cgagggggcg
     1561 caactcccgc gcgtactgga tgatcccaaa gttgatgtag cgattatcag caccacttac
     1621 attcagcaga ccgggctttc tccggtgcac gacagcgtat ttattgaaga taaaaattcg
     1681 ccgtatgtga atattttggt ggcacgggaa gataataaga atgcagaaaa cgtgaaggaa
     1741 tttctgcaat cttatcaatc atccgaagtc gctaaagcgg cagaaaccat ctttaacggt
     1801 ggcgcggtgc ctggctggta attctcactc atcccgcgcc ggacgattta tttttctggc
     1861 gcggcgacgg gaatccattg aaatcaaaat taccgatgac agaatcagca gcgttcccag
     1921 ccagtccggt aatgtgaacg tgatccccag caacagcaaa gagagtagcg cgctacttaa
     1981 tggttctgca cagctcaaaa tgctggcttt tggaccgcca attaattgtg ctcctttcag
     2041 gtacaggcta aatgtcagag atgtaccaat gaccaccaaa taaaaaaacg ccagaatcaa
     2101 actgccgtta acgacaaagt ttgtcccttg tctggcataa aaaggcaaca gaatcagacc
     2161 gccaatcagc atactccagc cgacgactgg taacgtgcca tagcgggcaa tcagcgttga
     2221 gggataggtg gtatagaatg cagcagcaaa tgccgaggca atgccccaga acaacgcgga
     2281 aggagagatc gataatgacg tcggattacc gtgtgtcacc aataaaaaag ttccgaccag
     2341 cgatgtcaaa atagcgcaga aaaccagaat gcccgggcgg gatttacgca ccagtgagaa
     2401 ccaggcgacg ataatcgtcg gtgagaggaa ttgcagcacc gttgccgtgg cagcattcga
     2461 tttttcgatg gttagcaaaa aagtgagctg tacggttagc gcgccaacca cggaaaaaat
     2521 cagcagacta atggcatctt tatgattgtt aataatagaa aagattttat cgccatgaac
     2581 aaatgacagc gtcagcagaa tcaaaccggc gaatatcaaa cgcgtcatag tcaaaaactg
     2641 cgatgacatc tggctttgct ccatgatgta ttgcgcacag acccctgaac ttccccacaa
     2701 cacggcggca atcagaacgt tcagcatccc ctttctggtg gaacccattt ttccctctgc
     2761 atactgtttt gtcgtttatg tatgcaggca tgatagcaaa aatggcgagg atggctctgt
     2821 ttgcgccacg ccgatttttc ttagcccaga ctggtgagct aaatatcttt aataaacagc
     2881 aagcaaatca tcgcaatacc caacatccct atcgccagcc agaacaacgc atggtagctc
     2941 cagatttcaa ccataattcc gtcaacagag ccggcgataa tccagccgac gcgtgaagta
     3001 tttgcatata acgttgtcgc agagcctatt ttttcaggca tcaggtcctg aaaataaagc
     3061 atgccgatac cacagagaat accaaggaag atggcattta gaatttgcaa ttccagctca
     3121 actgccggag tcgtcgccat gagtacgctg gcgtaaaaac acattccact cacgatagca
     3181 atgagcatta atagtcgctt gccaatacgt ttcatgtaat agcctgcgat taacatcatc
     3241 ggaatttcca gaccggcagc gataccaatc atttctccag ccagtttatc ggttagatgc
     3301 agttcatcaa taataaatag cggcatattt atcatgtaga gattattcgc cgcccacatc
     3361 attgaacaga ccacaaaaag tagccgcgta tctcgctttc tatggatgga gggtaaaatt
     3421 tctacgggtt gggtaacgac aggaacattt ctttgtattg atggcaaaaa caaccagact
     3481 atcagcccgc aaacaacaaa tgcgatggca gcggtgagat acatcacttt aaaactaaat
     3541 cccattgcca gttcataagc gagcggtggc ccgataaccc aggcaagcga gatctgagca
     3601 cgtaaaaatg tactgaacat gaccgtctca cggcctgttc tatcggcgtg ttcacgggcg
     3661 agggcgaaca tttgcgggtt tgcggtggaa gcaaaactac tcagaagtac gcccgttgag
     3721 agcagaatga agtagttgcg attccacgca aaaagcgtgc aggccagcac tccaaataag
     3781 caacatagca ggatcagtaa tttacggtcg ccttgtttat cggagttcct tgccagaaat
     3841 tgactgacca gaattcccat aatagcgcta ccggtgaaga aaaaacctac cattatagga
     3901 cgggctttca gttcatctgc gaggaatata cttagggtag gagtctgaag agcgcccgca
     3961 atacccgtca gaaaggcgac aagtaaaaat gccgcggcag tgagatcaag tatttttgat
     4021 ggagtggtag ccgttttttg cattcttctg aatcaccggg cgacaaaata
//
LOCUS       DAZKFU020000329         4027 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZKFU020000329 DAZKFU020000000
VERSION     DAZKFU020000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.331"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(286..1443)
                     /gene="intS"
                     /locus_tag="ACHOQQ_RS22505"
                     /old_locus_tag="pgaptmp_004501"
     CDS             complement(286..1443)
                     /gene="intS"
                     /locus_tag="ACHOQQ_RS22505"
                     /old_locus_tag="pgaptmp_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HGU4472659.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IISLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWIDEKVE"
     gene            complement(1605..1679)
                     /locus_tag="ACHOQQ_RS22510"
                     /old_locus_tag="pgaptmp_004502"
     tRNA            complement(1605..1679)
                     /locus_tag="ACHOQQ_RS22510"
                     /old_locus_tag="pgaptmp_004502"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1644..1646),aa:Arg,seq:cct)
     gene            complement(1755..2687)
                     /gene="yfdC"
                     /locus_tag="ACHOQQ_RS22515"
                     /old_locus_tag="pgaptmp_004503"
     CDS             complement(1755..2687)
                     /gene="yfdC"
                     /locus_tag="ACHOQQ_RS22515"
                     /old_locus_tag="pgaptmp_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGU4472660.1"
                     /translation="MDNDKIDQHSDEIEVESEEKESGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2981..3736
                     /gene="mlaA"
                     /locus_tag="ACHOQQ_RS22520"
                     /old_locus_tag="pgaptmp_004504"
     CDS             2981..3736
                     /gene="mlaA"
                     /locus_tag="ACHOQQ_RS22520"
                     /old_locus_tag="pgaptmp_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGU4472661.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          974 a          939 c          939 g         1175 t
ORIGIN      
        1 tatattttgt ttagcattga atgattatca caatgcgcta tctgtttttg gtttaattat
       61 ctgttattgt tttatgttgc ggttttactg tgtggttttt tatgcttttg tggtgttttt
      121 atctatttaa gcgccatgcc tttaaaggca tataagcgaa aatagcatga ggtttatcct
      181 caattgctat gttttttagt acaaaaaaga gggacaaaac tgagacacac atagctttgt
      241 actggattgc aaggctttgt gctcttcgat agtggttaag gtggatcact ccaccttttc
      301 atcaatccag tccgcccacc attgcatcat ttctctgcgt ttatcgagat actgagcatg
      361 gttgtaaatc ccacgcacag atccgccgtt ggcatgtgcc agttgcactt caatagcgtc
      421 agcaggccat tcgtgctcgt tcataattgt gctgaattca tgcctgaatc cgtgaccgct
      481 ttccagaccc tcatagccga tttgtttgat cacaagcaat accgcgttct cgcagattgg
      541 cttcttctta tcgttgcgcc cggcaaaaac aaactctgat actggtttag tgatggagct
      601 tagcgtagtg agaagttcaa ccacctggtc tgacatagga acaacatgaa tcttgcggcc
      661 cttcatcaca ctggcgtcga tagtgataat cctgttttca aaatcgacgt tcttccattg
      721 catggagcga agctctttcg ttcttagggc tgtgtagcgt aaaactttgg tcgcaatgag
      781 cgatatgatg cttcctgaaa atgttgccag tgctttgttg aatgcaggga tctggtctgc
      841 tgggagaaac gggaagttct tcttgcggta tcccttcatg gcgtcagcaa ggtcaggtgc
      901 cgggttatat ttagccctac cagtgacaat agcgtaacgg aaaacctcgc cgcatcttct
      961 gcgggctttg ttggctcgct ccattgcacc gcgatcttca aatctgcgga ttacttccag
     1021 cagttgcatc ggctcaatat cctgaatttc aaggccgccg atgataggta aaatgtcgtc
     1081 atcaaacatt tttgcaagtt cagttgcata gcctactgac cagacttgct tcttgtgctc
     1141 gtaccattcc ttgtaaatcg cactaaagga attgttgtta gacgaagcct ttttcgcttt
     1201 taccggatcg atgccaaccg agatgtcttt cctcgcggtc catgctttat ctcttgcctc
     1261 ctgcaaagtc attagcggat attttcctac ggtcaggatt ttctccttac cgtcaatctt
     1321 gtagcgaagc tgccatacct ttttccctga cacagggaca taaaggtaca ggccattacc
     1381 atcgagtagg cggtatggtt tttctttcgg ctttgctgct tcaatctgct taacggtgag
     1441 catgggtaaa aatccggtgg gtaaaattat tttatccact ttttacccgt catggagtgc
     1501 ggctgtcaac gatctgacgc gaaccattac gaactgtgaa tctacggaag gcttgatatt
     1561 caggggattt tgcggactgg tacggatggg agcgaactga taaatggtgt cccctgcagg
     1621 aatcgaacct gcaattagcc cttaggaggg gctcgttata tccatttaac taagaggaca
     1681 atgcggaatg agtatacccg ctaatggact gcggggtaag tacgctgccg tgcgattgct
     1741 taaaccctcg ccatttatgc cgggttttta tcatttttct taatgttttc cgcacgttct
     1801 gctttttggc gtgcttctgc tttgcgcttg ttgctcatgt cgttacggat ctgtgcatga
     1861 ctcattaacg cgaagataaa ggtgccgccg cagatgttcc ccgctaaagt aggtagtgcg
     1921 aagggccaga tgaaatcgct ccagtgcagc gtgccgttaa acaccagata gaggatttca
     1981 acagaaccga caacgatgtg ggtggtgtca cccagggcaa taagccaggt catcaatata
     2041 atcaccacaa tctttgccgc acccgctgca ggaaacatcc ataccatagt ggcgatcagc
     2101 cagccggaaa tgatcgcgtt ggcaaacatt tcgctggggg tgttcttcat cacatccatg
     2161 ccgattttga caaatgcatc gcgagtttct tcattgaaga ttggcatata ttcaaatgcc
     2221 cacgccgcaa tacctgtccc gagaatatta cccagcagca cgacgcccca taaccgcata
     2281 agtaagccaa cgttgctcat tgtcggtttt tgcatgacgg gtagtacagc agtcacggtg
     2341 ttttcggtaa ataattgctg gcgggccata atgacgataa taaaaccaaa ggtataacct
     2401 agattctcca gcaagaagct gcccggcaca ccttccagtt cgacatgaaa tatccctttt
     2461 gccagtaatg aagcgcccat cgacagaccc gccgcaatgg ctgaccacag tagtgccatt
     2521 gcgtcgcgtt ccagctcttt ttcaccatcc tggcggatat gctcatgaat tgccatcgcc
     2581 cgggagggga gtcgatcttc atctatttct atttttttgc cactctcttt ttcttcgctc
     2641 tcaacttcaa tttcgtcgct gtgttgatca attttgtcgt tgtccatggt ctcttctctt
     2701 gaattagcac gtatagctaa agcgtagcgg ctttttcgct cgcaactggc gggagttact
     2761 ctgaaaatgt agaaaaggcc gcgtttgcct ttttctgttt ctatagaatc aagtagccta
     2821 cagggcggcg attaccaggc tatgatcaaa tcagcaaatc agggcgtctg gacatcagtt
     2881 gacgtgctgt tacaatcgcc cacacctaaa caggcggata cggtatcgtt ccgtcatgga
     2941 tggcaaactg cataagccat aaaaaaacag ggagacattt atgaagcttc gcctgtcggc
     3001 gcttgctctg ggaactacgc ttctggtggg gtgtgcgagt tccggtacag atcagcaagg
     3061 gcgttctgac ccgttagaag ggttcaaccg caccatgtac aacttcaact tcaacgtact
     3121 agacccgtat attgttcgac cggtcgctgt cgcctggcgt gattatgttc cgcaaccggc
     3181 gcgtaacggt ttgagcaact ttaccggcaa ccttgaagaa cccgcggtga tggtgaacta
     3241 cttcttgcag ggcgaccctt atcaggggat ggtccacttt acccgctttt tcctgaacac
     3301 cattttgggg atgggtggtt ttattgatgt tgcagggatg gcgaacccga aactgcaacg
     3361 gactgaacct caccgcttcg gtagtacgct tggtcattat ggcgtgggtt atgggcctta
     3421 cgtccagtta ccgttctacg gtagcttcac gctgcgtgat gacggtggtg atatggcgga
     3481 tggtctttac ccggttctat cctggctgac ctggccgatg tctgtgggta aatggacgct
     3541 tgaagggata gaaactcgtg cgcagttgtt ggattccgac ggtctgctgc gtcagtcgtc
     3601 cgatccttat attatggtgc gcgaagcgta cttccagcgt catgatttca tcgctaatgg
     3661 cggcgaactc aaaccgcagg aaaatccgaa cgcgcaagcg attcaggatg atttaaaaga
     3721 tatcgattct gaataagaaa caaataaaaa aggtgagtcg caatactcac cttttttgtt
     3781 gttatccctc aattatcaca agtaaaagga gataaaccga caatgacccg gacgtaatta
     3841 tgtaaaaata ctgatgttcg tcactgacta tttccgatat tcagctgttg aaaaatcaaa
     3901 aaactggaaa aataatttaa atatcatcaa aattaacacc tagcgcatat agttgattaa
     3961 tttcattttg atatttcgtc tggcgatctt gaagcctttt tcaatgttac cttcgagttc
     4021 cttaaag
//
LOCUS       DAZKFU020000330         4016 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZKFU020000330 DAZKFU020000000
VERSION     DAZKFU020000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.332"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..546
                     /gene="ykgH"
                     /locus_tag="ACHOQQ_RS22525"
                     /old_locus_tag="pgaptmp_004505"
     CDS             <1..546
                     /gene="ykgH"
                     /locus_tag="ACHOQQ_RS22525"
                     /old_locus_tag="pgaptmp_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HGU4472662.1"
                     /translation="INKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQR
                     ITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIINWYASEYHNGRQVLDE
                     IQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLILGFFLSIV
                     ISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(789..1484)
                     /locus_tag="ACHOQQ_RS22530"
                     /old_locus_tag="pgaptmp_004506"
     CDS             complement(789..1484)
                     /locus_tag="ACHOQQ_RS22530"
                     /old_locus_tag="pgaptmp_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGU4472663.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1477..2904)
                     /locus_tag="ACHOQQ_RS22535"
                     /old_locus_tag="pgaptmp_004507"
     CDS             complement(1477..2904)
                     /locus_tag="ACHOQQ_RS22535"
                     /old_locus_tag="pgaptmp_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGU4472664.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLIAMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2915..3634)
                     /locus_tag="ACHOQQ_RS22540"
                     /old_locus_tag="pgaptmp_004508"
     CDS             complement(2915..3634)
                     /locus_tag="ACHOQQ_RS22540"
                     /old_locus_tag="pgaptmp_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGU4472665.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1066 a         1058 c          841 g         1051 t
ORIGIN      
        1 atcaataaag ataatataaa agtgagttac agcctaaaaa taaatcagac aacaccaggc
       61 atacttgtta actgtgatag caataataac tttgcctgtc agaccacaat gactgaagat
      121 gttattcagc gaattactac attttttcac accagcccag atgtcaagaa cagagaaata
      181 aagctggaat ggtcaggaga taagagagat ttaccaactg ctgaagcgga aatatctcgc
      241 gtgcaggcgt ctattatcaa ttggtatgcg tcagaatacc ataatggcag gcaagttctc
      301 gatgagatac aaacaccttc agcaattaac agtgagcttt atacaaaaat gatatacctg
      361 accaggaact ggtcattgta tccgaacggt gatggctgtg taactataag ctcaccagaa
      421 ataaaaaata aataccctgc tgccatttgc ctgattctgg gattttttct aagcattgtg
      481 atttctgtaa tgttttgcct tgtcaaaaaa atggtagatg aataccaaca aaactctggg
      541 cagtaataaa cagcattctg atagagagtg cctgtataaa gctgaagtga caatgaccgg
      601 gaagggaacc cgttttcagg aatggatgcc tgtctttttc actgtaaata tcctggcatt
      661 actaatgcca ggatatttac agaagaggag cataaccatg acggaacggg gttcagtcag
      721 gttgtcctta acgctattca caacctcgta tgccgccttc aggtttatgt ccagacttca
      781 tatctctctc agcaatcctc aataatcaga tacaccgctt tcaccgggcc atgaaccccg
      841 acgactttga taagctcaat atccgccgtt gaactagggc cgctaatgat gttaatgcag
      901 gacggcatgc gttcaccggc ctgcgctttc tgatgcaatt tttctgcgag ttgcgctaca
      961 cgcggcagta ttgtgctttt acgcaggata aaaagagaag attccgggag caggctcaat
     1021 gaacgcccgc gctcggcggc ggaaaaaaga accacgcctc ccgattcggt taaaccatat
     1081 tcagcataca cgacacccac tttagcctgc tctgcctgcg agatattctc ggcacctttc
     1141 gccggatccc aaacaacggc attgcattcc tgctgcaaac gttcgctaat ccccaattcc
     1201 tccagcctcg tgtcaccgct aatcacgacc gactgatctc ccagctcttt acacagacgt
     1261 attgcagctt ctgccgcctt cgcctcgctg gtcagctcac agcgcgtcaa cataacatcg
     1321 ctggcaaact gaataaacgc gtcacagcgc tgctgttggt taagttgggt aagccgctcg
     1381 ttagcatagt tgttaagcgg cgcatcttct gcttgcggtt caagtcgcaa cgggcgaccc
     1441 agtgcctgag caacgttatt caaaaattcg cctcgattat ccattctttt tctcctgcgc
     1501 ctgatgtttc ttaaaccaac tacggaaact ctctccgtca gcttcaggaa gatcgcgtgc
     1561 ttccatccag tcgctaatcg cgccaaattt gagtggtgtt ttgccgccat tgataaacca
     1621 gcttgccgca tgagcgccgg ccatcatccc gactttccac aatcctggat gactattggc
     1681 ataagcgaac atttttatcg cccgttgctc tgcttttgcg gtgatccctt tttcagccat
     1741 cacccgacga tgacgcaaaa tcagttttga cagcggaata cgcaccggac acacgctgtc
     1801 acaagcagtg cataaagagc aggcgtaggg taaatcttta aaatctttat agccgccaag
     1861 tagcggagaa atcaccgcac caattggccc tggataaata gagccatatc catgaccgcc
     1921 aatatggcga tatgccggac aagtattcat acaagccccg cagcgaatac agcgcagcac
     1981 atcccgaaat tcagaggcca gcacctcaga acgcccgtta tcgacaataa ccagatgaaa
     2041 ctcttcagga ccatcaacgt gccccgcttc gcgcggtcct gtcagccagg tgttgtatcc
     2101 cgtcaaacgt gcaccaacgg cactgcgcgc cagcatggcg atcaatacat ctacctcggc
     2161 aaacgtgggg gcaatacgct ccattcccat cactgcaata tgcgttttag gcagcgtggt
     2221 acacattcgc gcattacctt cattggtcac caggcatacc gaacctgtct ctgccaccgc
     2281 gaaattacag ccggtaatac ctatttcagc actgaggaaa tcttcgcgga ttttttgccg
     2341 gatgaataag gtcatcgctt caggcgtttc cggcccctca tagcccagac gttcgtggag
     2401 cactcgacgg atctgatggc gatctttatg aattgccggg accacaacat gagatggcgg
     2461 atcttgatcc agctggagaa tatattcacc cagatcggtt tcaatcacct gaatgccagc
     2521 atcctgcaac acatgattga caccaatctc ttcggtcacc atcgatttag atttcaccac
     2581 cttccgggca tttttgcgtt gggcaacctg taaaatgtag cgggtagcgt cttctttggt
     2641 ttttgcaaaa tagacgtgac cgccgttttg cgtcactttt tctgagagct ggtacagata
     2701 agcgtcgaga ttactcagaa catgatcacg tatctgggcg gcccgatcgc gccactcctc
     2761 ccagtgcccc aattcatcga ccattttttg ccgatttgcc ccaatacgct gctgcgcgtt
     2821 tgccaccgct ttgcgcatga tcggatcttc aatttgctga cggatgcgtg tcttaaaatc
     2881 tgtattactg gttttgatcg acatctttat atcctcagcg gctcatcaac acttcagcaa
     2941 tatgcatcac tttgactttc tgcccttccc gttgtaatcg cccactgatg tttagcaggc
     3001 aactcacgtc agcaccaatt aaatactcag ggcggacttc catcaggtgc gcaacctttt
     3061 ctttcaccat ctcgccggat atttcggcca ttttgaccga gaacgtgccg ccaaatccgc
     3121 agcaggtatc ctgttcagca aaggtcaaca gctccagtcc acgcacattt ttcagcagcg
     3181 taagtggctc gtccttcact cccagcttac gggccaggct acaagatggg tgatacaccg
     3241 ctctcccttg caaactggca cctacatcga ctacccctaa tttattaaca ataaaagagg
     3301 tgagatcctg catacgcgcg gcaacctttg cggcacgtga tgcccattca ggttcatccg
     3361 ccagatacat cgggtaactt tttacggcat aggtgcagga gccagccggt gaaataatgg
     3421 gatcatcgtt atcctccagt gcggcgatca gatttttcat ccctggaatc gcttctttga
     3481 tataaccgct attgatcgca ggctgaccgc agcatccctg tttctccggg aaatttacgc
     3541 gacagccgag tttttccagt agcagcacgg agtctcgtgc cattcttgat ttcagggcgt
     3601 caccaataca ggtgacaaag aaattgacat tcacaactaa tactccatta cttcatgccc
     3661 atttatgccc gatgtttccc cccaccgggg gatcaatcat caataaatat ggcgttcttt
     3721 tttaattggc tagcaacata ccacccctca tttgtatgac aaatgataat ctttcatcca
     3781 aacttcaaat gcaataacta attttatttc tttcggcatc gctcacattt tatggggaaa
     3841 tcgacgatca tttttggcat aagaaatcca tttaaaaatg gctacatccc ttatacctat
     3901 tcagagctac ttattcaggg gttattgaga tatacactga tggtgtacaa aaaaatcacc
     3961 gacaataatc acgtatcaag taatagtcgg gattttattt aattgaagca atttag
//
LOCUS       DAZKFU020000331         4015 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZKFU020000331 DAZKFU020000000
VERSION     DAZKFU020000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.333"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(155..2188)
                     /gene="metG"
                     /locus_tag="ACHOQQ_RS22545"
                     /old_locus_tag="pgaptmp_004509"
     CDS             complement(155..2188)
                     /gene="metG"
                     /locus_tag="ACHOQQ_RS22545"
                     /old_locus_tag="pgaptmp_004509"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGU4472666.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2320..3429
                     /gene="apbC"
                     /locus_tag="ACHOQQ_RS22550"
                     /old_locus_tag="pgaptmp_004510"
     CDS             2320..3429
                     /gene="apbC"
                     /locus_tag="ACHOQQ_RS22550"
                     /old_locus_tag="pgaptmp_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGU4472667.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3691..3972
                     /gene="yehE"
                     /locus_tag="ACHOQQ_RS22555"
                     /old_locus_tag="pgaptmp_004511"
     CDS             3691..3972
                     /gene="yehE"
                     /locus_tag="ACHOQQ_RS22555"
                     /old_locus_tag="pgaptmp_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HGU4472668.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT          992 a          998 c         1021 g         1004 t
ORIGIN      
        1 atataactct tcatccgcgt tccctgtatt tatatccagt tataagcgga tgctagcagt
       61 taatgaaaaa aattaagagc gttgaacaaa caagaaaaca actttaggaa tttataaaga
      121 aaaggcgctg tcgatgcagc gccttgaagg gggattattt cacctgatga cccggtttag
      181 caccggcatc cgggcttagc aggaaaatat ctttcccgcc aggaccggca gccatcacca
      241 tgccttcaga gataccgaag cgcattttac gtggtgccag gttagccacc ataatggtgt
      301 gacggccaat cagtgcctgc ggatccgggt aagcagaacg aatgccggag aagacattgc
      361 gtttttcacc gccgagatcc agcgtcaggc gtagcagttt gtcagaacct tcaacaaact
      421 ctgcgttttc aatcagcgcc acgcgcaggt caactttggc gaagtcgtca aaggtgatgg
      481 tttcctgaat cggatcatct gccagtgggc cagtaactgg cgcggcagct gctttcactt
      541 cttctttaga cgcttccacc agtgcttcaa cctgcttcat atcgatgcgg ttatacagcg
      601 ccttgaacgg attcactttg tggcccagca gcggttgctg gataccatcc caggtcagtt
      661 ccgtatttag gaatgcttct gcgcgctcgg tcagtttcgg cagtaccggc ttcaggtaag
      721 tcatcagcac gcggaacagg ttgatgccca ttgagcaaat cgcctgcagg tcagcatcgc
      781 ggccttcctg ttttgccacc acccacggag cctgttcatc gacatagcgg ttagccagat
      841 cagccagcgc catgatttcg cgcactgctt taccaaattc gcggctttcc cacgcttcac
      901 caatcacttc agcggcatca gtgaaggttt tgtacaactg cgggtcagcc agttcgcttg
      961 ccagcacacc gtcaaaacgc ttgttgataa agcccgcatt acgagaggcc aggttaacca
     1021 ctttgttaac gatatcggca ttcacgcgct gaacgaaatc ttccaggttg agatcgatat
     1081 catcaatgcg cgaagagagt ttcgcagtgt agtagtaacg caggctgtcg gcgtcaaaat
     1141 gattcagcca ggtgctggct ttaataaagg taccgcgaga cttggacatc ttcgcgccgt
     1201 tcaccgtcac atagccgtga acaaacaagt tggtcggctt gcggaagttg ctgccttcca
     1261 gcattgcagg ccagaacagg ctgtggaagt aaacaatatc tttaccgatg aagtggtaca
     1321 gctcggcggt ggagtctttc ttccagtatt catcgaagct tacgctgtcg ccgcgcttat
     1381 cgcacagatt cttgaaagaa cccatgtagc caatcggtgc gtccagccag acgtagaaat
     1441 atttgcccgg cgcgttcgga atttcaaaac cgaagtacgg tgcgtcgcgg gagatatccc
     1501 actgttgcag gccagattca aaccactcct gcattttgtt tgccacctgc tcctgcaacg
     1561 caccgctgcg ggtccatgcc tgcaacattt cgctgaaaga gggcagatca aagaagaagt
     1621 gttcagaatc acgcattacc ggggtagcgc cagaaaccac cgatttcggc tcgatcagtt
     1681 cagtcgggct gtaggtcgcg ccgcagactt cgcagttatc gccgtattga tccggggatt
     1741 tacatttcgg gcaggtgcct ttcacaaaac ggtccggcag gaacatgcct ttttccggat
     1801 cgtacagctg agagatggtg cggtttttaa taaaaccgtt ttctttcaag cgagtataga
     1861 taagctcaga caactggcgg ttctcttcgc tgtgcgtcga gtgatagttg tcatagctga
     1921 tgttaaagcc tgcgaaatca gtctgatgct cctgactcat ttcgccaatc atctgctccg
     1981 gggtgatacc aagctgctga gctttcagca tgatcggtgt accgtgggca tcgtcggcgc
     2041 agatgaagtt gacctcgtgg ccgcgcattc gctggtaacg gacccagaca tcagcctgga
     2101 tgtgctccag catatggccg aggtggattg agccgttagc gtacggcagt gcgcacgtca
     2161 ccagaatttt cttcgcgact tgagtcatag taggcattac ttcttaatag tgaaaagggg
     2221 ttagatagta ccaaatggga aaatgttaag taagtacttc tctatattct tcaggtaaac
     2281 tatcagctga tggtttatct atacaaaaaa ggagtcggga tgaacgaaca atcccaggcc
     2341 aaatcaccag aagccttgcg cgcaatggtc gccgggacac tggccaattt tcagcaccca
     2401 accctgaagc ataacctcac cacgctgaaa gcgttgcacc atgtcgcctg gatggacgac
     2461 acgttgcatg tggaactggt tatgccattc gtctggcata gcgcgtttga agagttaaaa
     2521 gagcaatgta gcgccgaact gctgcgtatc accggggcga aagctatcga ctggaaactg
     2581 tcgcacaata tcgccacctt aaaacgcgtg aagaatcagc cgggtattaa tggcgtgaag
     2641 aacatcattg cggtcagttc cggtaaaggc ggcgtgggta aatcctccac ggcggtaaac
     2701 ctggcgctgg cactggcggc ggaaggggcg aaagtcggca ttctggacgc cgatatctac
     2761 ggcccatcaa ttcccaccat gctgggcgca gaaaatcagc gtccaacttc gccggacggt
     2821 acccatatgg caccgattat gtctcatggc ctggcaacca attcaattgg ttatctggtc
     2881 actgacgaca atgcgatggt gtggcgtgga ccgatggcga gcaaggcgtt aatgcagatg
     2941 ttgcaggaaa ccttgtggcc ggatctcgac tatctggtgc ttgatatgcc gccaggcacc
     3001 ggtgacattc aattgacgct ggcgcaaaac attccagtaa ctggtgcagt cgtggtcacc
     3061 accccgcagg acatcgcgtt gatcgatgcg aaaaaaggca ttgtgatgtt cgagaaagtc
     3121 gaggttccgg tgctcggtat tgtcgagaac atgagtgtgc atatttgcag taactgtggt
     3181 caccatgaac cgattttcgg taccggtggt gcagagaaac tggctgaaaa ataccataca
     3241 cagctgctgg ggcagatgcc gttacacatc tccttacgtg aggatctcga taaaggcacg
     3301 ccaacggtta tcagccgtcc ggagagcgaa tttaccgcta tctaccgcca gctggctgac
     3361 cgcgttgcag ctcagctcta ctggcagggt gaagtcattc caggcgagat ttccttccgc
     3421 gcggtctaac gcctattaac cattctggtt attcttcata caccgcagag atgttatatt
     3481 gatgcggtgt atttgttttt gccgcattaa tgaacactta tttaatactg cgaatattct
     3541 gcggaccttc gtccgaaact ctacaaagtc gattgatcgt acaggcaatg cttcattatc
     3601 atccttcgtg ctgtgtagtg gatgtacagc tatagtttgt tatgcactca ttctatccag
     3661 gcataacctt tactacaggg ataacgttca atgaataagt attggctgtc aggaattatt
     3721 tttcttgcct atggattggc ttctcccgcg ttctcttcag agactgccac attaacgatt
     3781 aatggcagga tcagtccccc aacatgcagt atggctatgg ttaatagtca acctcaacag
     3841 cattgtggtc agttaacgta taatgtcgat acccgtcatc aggtttcttc gcccgtcaaa
     3901 ggtgtaacga cagaagtggt tgttgcaggt agtgatagca aacgtcgaat cgtgttaaat
     3961 cgctacgatt aatttggcaa cctgatttta attgcattta gaaagccgaa atcat
//
LOCUS       DAZKFU020000332         3972 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZKFU020000332 DAZKFU020000000
VERSION     DAZKFU020000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.334"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..637)
                     /gene="hyi"
                     /locus_tag="ACHOQQ_RS22560"
                     /old_locus_tag="pgaptmp_004512"
     CDS             complement(<1..637)
                     /gene="hyi"
                     /locus_tag="ACHOQQ_RS22560"
                     /old_locus_tag="pgaptmp_004512"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HGU4472669.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRG"
     gene            complement(650..2431)
                     /gene="gcl"
                     /locus_tag="ACHOQQ_RS22565"
                     /old_locus_tag="pgaptmp_004513"
     CDS             complement(650..2431)
                     /gene="gcl"
                     /locus_tag="ACHOQQ_RS22565"
                     /old_locus_tag="pgaptmp_004513"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HGU4472670.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2521..3336)
                     /gene="allR"
                     /locus_tag="ACHOQQ_RS22570"
                     /old_locus_tag="pgaptmp_004514"
     CDS             complement(2521..3336)
                     /gene="allR"
                     /locus_tag="ACHOQQ_RS22570"
                     /old_locus_tag="pgaptmp_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HGU4472671.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNSNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3414..3896)
                     /gene="allA"
                     /locus_tag="ACHOQQ_RS22575"
                     /old_locus_tag="pgaptmp_004515"
     CDS             complement(3414..3896)
                     /gene="allA"
                     /locus_tag="ACHOQQ_RS22575"
                     /old_locus_tag="pgaptmp_004515"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HGU4472672.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
BASE COUNT         1005 a         1109 c          912 g          946 t
ORIGIN      
        1 cgccgcgatg cggattatca gcaatttgca ggtgaccaat tttatcggcc cactgagtca
       61 tggtgttggt taattcacct tccatccgct gcatgtgata aatgtcatac tgaattttca
      121 aattgcagca accaacatca tcaatcaatt tcagcgcctg ccgagttccg gtgagatgga
      181 aaccaggaat atcaaaatgg ttaataggtt caatcagtaa taaaatatct tctttcatca
      241 gcatattcgc ggcataacgc aggttttcta caagcgttgc gtgaatctgt tcgctgctga
      301 aaccagccgg cgttttaccg accagacagt taattttttt attacccagc gcacgggcat
      361 aacgaatcgc tgctgctacg ccatcccgaa actcttcttc acggccagga atacaggcaa
      421 taccgcgctc ccccgccgcc cagtcacccg ccggtaaatt gtgcagcgta tgttcgagtt
      481 tattactcgc cagcacctgt tttaattctt caatgtcgta gtcataagga aacataaatt
      541 caacgccgcg aaaaccacac tgcgcagctt tctcaaaacg ggcgagaaaa tcatattcac
      601 caaataacat cgataaatta gcagagaaac gtaacattat ttatctccct tattcatagt
      661 gcatgaagca ggtttcagtc ggtgcatccg ctgcgttatc ggcgatatct tcaaattcca
      721 tgacgttatc cagttcgctg cccatcgaaa tatttgtcac acgctcgaga ataacttcca
      781 cgactaccgg tacccgatat tgcgccatta aggctttcgc ctgttcaaag gccggtgcaa
      841 tatcttccgg tttgaagacg cgaatagctt tacaacctaa accttccgct acttttacgt
      901 ggtcgacgcc gtaaccgttc acttcgctgg agttgatatt ctcgaaagcg agttgcacgc
      961 agtagtccat gtcaaaggcg cgttgtgact gacgaatcag gccgagatag gcgttattca
     1021 ccagcacatg gatgtacgga atgttgaact gcgcgccaac ggctaactct tcaatcagga
     1081 actggaagtc gaagtcaccg gaaatcgcca ccacattgcg tttcggatca gcggcacaaa
     1141 cccccagcgc cgccggaatc gtccagccta acggaccagc ctgaccacag ttgatccagt
     1201 ggcggtcttt aaagacatgc agcatttgtg ccgcagcgat ttgtgacagg ccaatggtgg
     1261 tgacataaca aacatcgcga ccaaaggctt tgttcatctc ttcatacacg cgctgcggtt
     1321 tcaccggcac gttgtcgaaa tgggttttgc gcagcaaagt gcgtttgcgc tgctggcagt
     1381 cggcgaccca ttctttacga cacggcagac gacccgcttt ttgcatctcc tgcgccactt
     1441 caaccagcag tgtcagcgcc gctttagcat cagagacaat gccgagatcc ggacacagca
     1501 cgcgaccaat ttgcgtcggc tcaatatcga tatgcacgat tttgcgccct tcggtgtatt
     1561 tctctaccga accggtatga cggttagcaa aacggttacc gataccaaac accatgtcgg
     1621 acgccagcag cgttgcgtta ccgtaacgat gcgcggtttg cagacccacc atcccggcca
     1681 tcagttcatg atcgtccggg atacagcccc agcccatcag cgttgggatc accggaacgc
     1741 tggttagttc agcaaactgt tgtaacagtg cagctgcgtc agcattgatt accccgcccc
     1801 cggcaacaat caccggacgt tcggcctgga ttaacatttc tacagctttt tcgatctgca
     1861 tacggctggc agcaggtttg tagaccggca gcggttcgta catgtcagga tcaaactcga
     1921 tttccgcaac ctgaacgtcg aacggtaaat ccaccagtac cggacccgga cgaccagaac
     1981 gcatcaggtg aaatgcctgt tgcagcacgc gaggcaccag cgccgcttca cgtactgtaa
     2041 ccgccatttt gctgaccggt ttagcaattg cttcaatatc aacagcctga aaatcttctt
     2101 tatgcagacg ggcgcgcgga gcctggccgg taatgcacag aataggaatg gaatcagcag
     2161 aagcagaata gagcgcggtg atcatgtccg tgcccgcagg accggaagtc cccagacata
     2221 cgccgatatt ccctgccgtt gcgcgggtat aaccttccgc catgtgcgaa gcaccttcca
     2281 catgacgcgc cagaatgtga cgaataccgc cgtgcttacg catcgctgag tagaacggat
     2341 tgattgcagc tcccggaaca ccgaaggcgg tggtgatacc ttctttctcc agcacataca
     2401 ttgccgcgtc aacggctctc atttttgcca ttttattcct acctccatta attggaaaaa
     2461 ttttccaact ttcaaatttt gtcaaaaaaa cccgctccgc agagcgggat gcgatagaca
     2521 ttatggatgt gctttcagtc ccaacgccgt gctgatatcg cgggcggtgt ctctgaccag
     2581 ctcaccctga ctgacaaaac gatcttctgt cagtcttgat gaaggcccgg agatggagat
     2641 agcggcaaca acactaccca catcatcgta aattgctgaa gctatgcaat tcagacctac
     2701 aacatgctct tctttatcta cggtatagcc cagttcacgc gcttgttcca ggtccttcag
     2761 cagggtgggc atatccacaa gcgtagttgg cgtaaactgc tgcaaaccgg tttgcagaat
     2821 gatgctcatc aactcctctt ccgccagcgg ataaagcagc gctttgcccg caccggaagc
     2881 atgcagtggc agacgactgc ccagtggcgc acacatcctg accatcgatt tacactctaa
     2941 ctgaccaatt aataccgctt cattgctgtt acggatcgcg acattgaccg tttcgccaga
     3001 aagtaacatc aggcggcgca taaacggccc ggcgacggag aggacatcgc ggttatggat
     3061 gtacgctgca ccgacgttaa agacacctaa tcctatatgc caccagccta actgactgtc
     3121 ctgatagaca aaatccgctg cctgtaaaac cttcagcaag cgaaaggtcg tggagagcgg
     3181 caaatccaga ttgagagaaa tatcgctaac cgacgaactt cccccacttt tttccagata
     3241 ttgcagaatc gcaattcccc gctctaatgc ctgtgcgccc ttctgtgcca caggctccgc
     3301 ctgtcctggc ctgccgcgcc gtctaacttc cgtcatacct acgttaactc cgacctacaa
     3361 gtgagcataa acaatgaaca attagtttat cttatgcaag tcggttgcag gcgtcacgca
     3421 aaacagagtt cctgttcagg aatactttca acatcacagt tgtcactgcc gccgcgatcg
     3481 atggtcagaa aatcggtgac gcgctgccag gcgaaaagtg ggtgatgcca gacgttacga
     3541 tggtaattca ctccctgttc gccgttggta ataaacgccc gcagggttga caggtctggt
     3601 ttgtcgtcac ctaacgccac gaccaccaca aacacctcac ctttcatcgg gataaaggcc
     3661 tgagtaccca gcggatgacg ctcgagttcg tgaatggtca gcggcagatt cgccggttgc
     3721 gcgcggttaa tgctgataag cgtacggtct tgctcaagaa tctcaaccag cgccaaatcg
     3781 tggtaacgct ccaccaggcc attgttaata tggaaaaaat cccgttgctg cgtttcgatt
     3841 acgtcgccat aagcactaaa ggcttcctga cttaacggta atacctgaag tttcatcttt
     3901 ttgtccttgt aacataattc cgttcaacca ccgccgaccg gtcagcttgg tttcaataac
     3961 aggcaatcta aa
//
LOCUS       DAZKFU020000333         3966 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZKFU020000333 DAZKFU020000000
VERSION     DAZKFU020000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.335"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            116..319
                     /gene="aaeX"
                     /locus_tag="ACHOQQ_RS22580"
                     /old_locus_tag="pgaptmp_004516"
     CDS             116..319
                     /gene="aaeX"
                     /locus_tag="ACHOQQ_RS22580"
                     /old_locus_tag="pgaptmp_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGU4472673.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            327..1259
                     /gene="aaeA"
                     /locus_tag="ACHOQQ_RS22585"
                     /old_locus_tag="pgaptmp_004517"
     CDS             327..1259
                     /gene="aaeA"
                     /locus_tag="ACHOQQ_RS22585"
                     /old_locus_tag="pgaptmp_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGU4472674.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            1265..3232
                     /gene="aaeB"
                     /locus_tag="ACHOQQ_RS22590"
                     /old_locus_tag="pgaptmp_004518"
     CDS             1265..3232
                     /gene="aaeB"
                     /locus_tag="ACHOQQ_RS22590"
                     /old_locus_tag="pgaptmp_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGU4472675.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPINEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3324..3596
                     /gene="yhcO"
                     /locus_tag="ACHOQQ_RS22595"
                     /old_locus_tag="pgaptmp_004519"
     CDS             3324..3596
                     /gene="yhcO"
                     /locus_tag="ACHOQQ_RS22595"
                     /old_locus_tag="pgaptmp_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGU4472676.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3652..3915)
                     /gene="yhcN"
                     /locus_tag="ACHOQQ_RS22600"
                     /old_locus_tag="pgaptmp_004520"
     CDS             complement(3652..3915)
                     /gene="yhcN"
                     /locus_tag="ACHOQQ_RS22600"
                     /old_locus_tag="pgaptmp_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGU4472677.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT          907 a          975 c         1061 g         1023 t
ORIGIN      
        1 atattaaata tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc
       61 ggcgacagat gctgaaaata ataacgcctg ctctctctat acaaccaagg tcaacatgag
      121 tctgtttccc gttatcgtgg tgtttgggct gtccttccca ccgatatttt ttgaattgct
      181 tttatcactg gcgattttct ggctggtgcg ccgggtactt gtgccaacag gtatctacga
      241 ctttgtctgg catccggcgt tgttcaacac cgcgctctat tgctgcttgt tttatttgat
      301 atcgcgactg ttcgtttgag gttgaagtga aaacactaat aagaaaattc tcccgtacgg
      361 ccatcacggt cgtattagtc attctggcct tcatcgcaat ttttaatgcc tgggtctatt
      421 acaccgaatc cccgtggacg cgtgacgcgc gctttagcgc tgacgtcgtt gcgatcgcgc
      481 cggacgtttc tggactcatt acccaggtga atgttcatga taaccagctg gtgaaaaaag
      541 gacaggtact gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag gaagcgcaag
      601 ccgatgttgc ttattatcag gtactggcac aggagaaacg ccaggaggcc ggacgtcgta
      661 accgtctcgg tgtgcaggcg atgtctcgcg aagagatcga ccaggccaac aacgtactac
      721 aaacggttct gcatcagtta gcgaaagcgc aagcgacccg cgatctggca aaactggatc
      781 ttgaacgcac ggtgatccgc gcgccagcgg atggctgggt gaccaacctc aacgtctata
      841 ccggtgagtt tattactcgt ggatcaacgg cggttgcgct ggtgaaacag aactctttct
      901 atgtactggc ctatatggaa gaaactaagc tggaaggggt gcgccctggg tatcgtgcag
      961 agatcacgcc gcttggcagt aacaaagtgc tgaaagggac tgttgatagt gttgccgctg
     1021 gggtcaccaa cgccagcagc acgcgtgacg acaaagggat ggcgaccatc gactctaacc
     1081 ttgaatgggt gcgtttggcg caacgtgttc cggttcgtat tcgtctcgac aaccagcaag
     1141 agaacatctg gcctgcgggc accactgcta cagtggtggt cactggcaaa caagatcgcg
     1201 acgaaagcca ggattcgttc ttccgtaaaa tggcccatcg cctgcgtgag tttggttaat
     1261 cacgatgggt attttctcca ttgctaacca acatattcgc tttgcggtaa aactggcgac
     1321 cgccattgta ctggcgctgt ttgttggctt tcacttccag ctggaaacgc cacgctgggc
     1381 ggtactgaca gcggcgattg ttgccgccgg tccggccttt gctgcgggag gtgaaccgta
     1441 ttctggcgct attcgctatc gtggcttttt gcgcatcatc ggcacattta ttggctgtat
     1501 tgccggactg gtgatcatca ttgcgatgat ccgcgcacca ttattgatga ttctggtgtg
     1561 ctgtatctgg gccggttttt gtacctggat atcctcgctg gtacgaatag aaaactcgta
     1621 tgcgtggggg ctggccggtt ataccgcgct gatcattgtg atcaccattc agccggaacc
     1681 attgcttacg ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg gtattgtgtg
     1741 tgcgattatg gcggatttgc tcttttctcc gcgatcgatc aaacaagaag tggatcgaga
     1801 gctggaaagt ttgctggtcg cgcaatatca attaatgcaa ctctgtatca agcatggcga
     1861 tggtgaagtt gtcgataaag cctggggcga cctggttcga cgcaccacgg cgctacaagg
     1921 catgcgcagc aacctgaata tggaatcttc ccgctgggcg cgggccaatc gacgtttaaa
     1981 agcgatcaat acgctatcgc tgacgctgat tacccaatcc tgcgaaactt atcttattca
     2041 gaatacgcgc ccggaattga tcactgatac tttccgcgaa ttttttgaca cgccggtaga
     2101 aaccgcgcag gacgtccaca agcagctcaa acgcctgcga agagttatcg cctggaccgg
     2161 ggaacgggaa acgcctgtca ccatttatag ctgggtcgcg gcggcaacgc gttatcagct
     2221 tctcaagcgc ggcgttatca gtaacacaaa aatcaacgcc accgaagaag agatcctgca
     2281 aggcgaaccg gaagtaaaag tagagtcagc cgaacgtcat catgcaatgg ttaacttctg
     2341 gcgaaccaca ctttcctgca ttctgggcac gcttttctgg ctgtggacgg gctggacttc
     2401 cggtagtggt gcaatggtga tgattgcggt agtgacgtca ctggcaatgc gtttgccgaa
     2461 tccacgcatg gtggcgatcg actttatcta cgggacgctg gccgcgctgc cgttagggct
     2521 gctctacttt ttggtgatta tccctaatac ccaacagagc atgttgctgc tgtgcattag
     2581 cctggcagtg ctgggattct tcctcggtat agaagtacag aaacggcgac tgggctcgat
     2641 gggggcactg gccagcacca taaatattat cgtgctggat aacccgatga ctttccattt
     2701 cagtcagttt ctcgacagcg cattagggca aatcgtcggc tgtgtgctcg cgttcaccgt
     2761 tattttgctg gtgcgggata aatcgcgcga caggactgga cgtgtactgc ttaatcagtt
     2821 tgtttctgcc gctgtttccg cgatgactac caatgtggca cgtcgtaaag agaaccacct
     2881 cccggcactt tatcagcagc tgtttttgct gatgaataag ttcccagggg atttgccgaa
     2941 atttcgcctg gcgctgacga tgattatcgc gcaccagcgc ctgcgtgatg cgccgatccc
     3001 gattaacgag gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag accatgtgat
     3061 atctgcccgt agcgatgata aacgtcgtcg gtactttggt cagttgctgg aagaactgga
     3121 aatctaccag gaaaagctac gcatctggca agctccaccg caggtgacgg aaccggttca
     3181 tcggctggcg gggatgctcc ataagtatca acatgcgttg accgatagtt aagtcaaaac
     3241 cgacgccaaa agcgtcggtt ttttcatggc tatacttagc gatgaacggc agaactcgcc
     3301 gcgaaacgtg acggtggcaa cagatgaata tttatacctt tgattttgat gagattgaga
     3361 gtcaggagga tttttatcgc gacttcagcc aagcctttgg tctggcgaaa gataaggtac
     3421 gcgatctcga ctcactatgg gatgtgttaa tgaacgatgt cctgccgcta ccacttgaga
     3481 ttgaatttgt tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta atattgctgt
     3541 ttgatgaggc agaggaagag ctggaagggc atttgcgttt taatgttcgt cattagcgca
     3601 aaaaaaagcc cccgaaccgg gggcaatatc gtcggacaag acgatgaggg tttatttgta
     3661 cagttcagcc gtagcgtgcc aggtgtcacc gctacgagct tcagtaatct ggtaggccgt
     3721 tgcgcctttc tcttccgctt ttttgttcag catttcacgc atatccattg gcgaagacgc
     3781 cacaccactt acggatacgg tcccgattgc ttcacgattt tgtgcttgtg cagcatcaat
     3841 ggagtcggca gcgaatgcac cgaaagagag aacagaaagt acgcttaatg cagcaacagt
     3901 ggttttgatt ttcatgattt ttacctcgac ataatctttt agctgggcct ttgtttcgtg
     3961 acccat
//
LOCUS       DAZKFU020000334         3965 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZKFU020000334 DAZKFU020000000
VERSION     DAZKFU020000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.336"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..511
                     /gene="asnC"
                     /locus_tag="ACHOQQ_RS22605"
                     /old_locus_tag="pgaptmp_004521"
     CDS             53..511
                     /gene="asnC"
                     /locus_tag="ACHOQQ_RS22605"
                     /old_locus_tag="pgaptmp_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGU4472678.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            601..1044
                     /gene="mioC"
                     /locus_tag="ACHOQQ_RS22610"
                     /old_locus_tag="pgaptmp_004522"
     CDS             601..1044
                     /gene="mioC"
                     /locus_tag="ACHOQQ_RS22610"
                     /old_locus_tag="pgaptmp_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGU4472679.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            1423..3312
                     /gene="mnmG"
                     /locus_tag="ACHOQQ_RS22615"
                     /old_locus_tag="pgaptmp_004523"
     CDS             1423..3312
                     /gene="mnmG"
                     /locus_tag="ACHOQQ_RS22615"
                     /old_locus_tag="pgaptmp_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGU4472680.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            3376..>3965
                     /gene="rsmG"
                     /locus_tag="ACHOQQ_RS22620"
                     /old_locus_tag="pgaptmp_004524"
     CDS             3376..>3965
                     /gene="rsmG"
                     /locus_tag="ACHOQQ_RS22620"
                     /old_locus_tag="pgaptmp_004524"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGU4472681.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGER"
BASE COUNT          997 a         1006 c         1021 g          941 t
ORIGIN      
        1 taaaaaagaa ggctaaaata gaatgaatca tcaatccgca taagaaaatc ctatggaaaa
       61 ttatctgatc gacaatctgg accgtggcat cctggaagca ttaatgggca atgcgcgcac
      121 cgcttacgcc gaactggcga aacaatttgg cgtcagtccg gggacgattc acgttcgagt
      181 agagaaaatg aagcaggcgg gaatcattac cggggcgcgt attgatgtca gcccgaagca
      241 gctcggttat gacgtaggct gctttatcgg cattatatta aagagcgcca aagactatcc
      301 ttccgcgctg gcaaagctgg aaagcctcga tgaagtgacc gaggcgtact acaccaccgg
      361 ccactacagc atctttataa aagtgatgtg ccgttcgatc gatgctctcc agcatgtact
      421 tatcaacaag atccaaacaa ttgatgaaat tcagtccacc gagacactga tcgtcctgca
      481 gaacccgatc atgcgtacca tcaagccctg atcggctttt ttaatcccat acttttccac
      541 aggtagatcc caacgcgttc acagcgtaca atacgccact cttaataaag gtggcggttt
      601 atggcagata tcactcttat cagcggcagc accctcggcg gtgccgaata tgtagcagaa
      661 cacctggctg aaaagctgga agaggcgggt tttaccaccg aaacgctgca cggtccgctg
      721 ttggaagatt tatctgcctc ggggatctgg ctggttatca gctccaccca cggtgccgga
      781 gatattccgg acaacctttc tcctttctat gaagcattgc aggaacagaa gcccgatctt
      841 tctgcggtcc gctttggcgc aatcggtatt ggtagtcgtg aatatgacac cttttgtggg
      901 gctatcgata aactcgaggc tgaactcaaa aattccggtg caaaacagac gggcgaaaca
      961 ctgaagatca acattcttga tcacgacatt ccggaagatc cggcagaaga atggctggga
     1021 tcatggatta atttactcaa ataagtatac agatcgtgcg atctactgtg gataactctg
     1081 tcaggaagct tggatcaacc ggtagttatc caaagaacaa cggttgttca gtttttgagt
     1141 tgtgtataac ccctcattct gatcccagct tatacggtcc aggatcaccg atcattcaca
     1201 gttaatgatc ctttccaggt tgttgatctt aaaagtcgga tccttgttat ccacagggca
     1261 gtgcgatcct aataagagat cacaatagaa cagatctcta aataaataga tcttcttttt
     1321 aatacccagg atcccaggtc tttctcaagc caacaaagtt gagtagaatc cacggcccgg
     1381 gcttcaatcc attttcatac cgcgttatgc gaggcaatca ccatgtttta tccggatcct
     1441 tttgacgtca tcatcattgg cgggggtcat gcaggcaccg aggccgcgat ggctgcggcg
     1501 cgtatgggtc aacagactct gcttttgaca cacaatatcg acactctggg gcagatgagc
     1561 tgcaacccgg cgatcggcgg tattgggaag ggacatctgg taaaagaagt ggatgcactc
     1621 ggcggtctga tggcgaaagc gatcgatcag gcgggtatcc agtttaggat actaaacgca
     1681 agtaaaggac cggctgttcg cgctacccga gctcaggcgg atcgtgtgct ctaccgtcag
     1741 gcggtacgta cggcgctgga gaaccaaccg aatctgatga tcttccagca ggcggttgaa
     1801 gatcttattg tcgaaaacga tcgcgtggtc ggtgctgtta cccaaatggg actgaagttc
     1861 cgtgccaaag ccgttgtgct caccgttggg acgttcctcg acggtaaaat tcatatcggt
     1921 ctggataact acagcggtgg ccgtgctggt gatccgccgt ccattccgct ttctcgccgt
     1981 ttgcgtgaac ttccgctgcg cgttggtcgt ctgaaaaccg ggacaccacc gcgtattgat
     2041 gctcgaacca tcgactttag cgtgcttgcg caacagcatg gcgataaccc aatgccggta
     2101 ttctcgttta tgggcaatgc gtcccagcat ccccagcagg tgccgtgtta tatcactcat
     2161 accaacgaga aaacccatga tgtgatccgc agtaacctcg atcgtagccc aatgtacgca
     2221 ggggtgatcg aaggtgtcgg cccacgctac tgcccgtcga tcgaagacaa agtcatgcgc
     2281 ttcgccgaca gaaatcagca tcagatcttc cttgaaccgg aagggctgac ctctaacgaa
     2341 atttatccga acggtatctc caccagcctg ccgttcgatg tgcagatgca aatcgtccgc
     2401 tccatgcagg ggatggaaaa cgcgaagatc gtgcgtccgg gttatgccat tgagtatgac
     2461 ttcttcgatc cacgcgacct gaaaccgacg ctggagagca agtttatcca ggggctgttc
     2521 tttgctggtc agattaacgg cactaccggt tacgaagaag ccgctgcgca aggtttgctg
     2581 gccggtctta acgctgcccg tctgtctgct gacaaagaag gttgggctcc ggcgcgttct
     2641 caggcctatc tcggcgtact ggttgatgac ctgtgcactt taggaaccaa agaaccgtat
     2701 cgtatgttta cttcgcgcgc agaatatcgt ctgatgctgc gcgaagataa tgcggatctg
     2761 cgtttgactg aaatcggtcg tgaactgggc ctggtggatg acgaacgttg ggcgcgcttt
     2821 aacgagaaac ttgagaatat cgagcgtgag cgtcagcgtc tgaaatcgac ctgggtaacc
     2881 ccgtcggcgg aagctgcagc cgaagtgaat gctcacctga ctgcgccgct ttcccgtgaa
     2941 gccagtggtg aagatctgct gcgtcgtccg gaaatgactt atgaaaaatt aaccacgctg
     3001 acgccgtttg cccctgcgtt gacagacgaa caggcggcgg aacaggttga gattcaggtt
     3061 aaatacgaag gttatatcgc gcgccagcaa gatgagatcg aaaagcagct gcgtaacgag
     3121 aacaccctgc tacccgcgac actggattac cgccaggtat ccggtctttc taacgaagtg
     3181 atcgccaaac ttaacgatca caaaccggcc tctatcggcc aggcttcacg tatttctggc
     3241 gtcacgcctg cggccatctc cattctgctg gtgtggctga aaaaacaggg tatgctgcgt
     3301 cgtagcgcat aacgcattaa aaatgcctgg taagcacccg cttaccaggc aacgcatcaa
     3361 gaacaggtaa tcaccgtgct caacaaactc tccttactgc tgaaagacgc aggtatttcg
     3421 cttaccgatc accagaaaaa ccagcttatt gcctacgtga atatgctgca taaatggaac
     3481 aaagcgtaca acctgacttc ggtccgcgat cctaatgaga tgctggtacg ccatattctc
     3541 gatagcattg tggtggcacc gtatctgcaa ggtgaacggt ttatcgatgt cggcaccggt
     3601 cctggactgc ctggcattcc actctctatc gtgcgtcctg aagcccattt cactctgttg
     3661 gatagccttg gtaaacgcgt gcgtttcctt cgtcaggtgc aacatgagct taaactggag
     3721 aatattgagc cagtacagag cagggtagaa gagtttcctt cagagccgcc atttgatggc
     3781 gtaattagcc gcgcttttgc ctctctgaac gatatggtga gctggtgcca ccatcttcct
     3841 ggtgagcaag gccgtttcta cgcgctgaaa gggcaaatgc cggaagatga aatcgctttg
     3901 ttgcccgaag aatatcaggt tgaatcagtg gttaaacttc aggttcctgc cctggatggc
     3961 gaacg
//
LOCUS       DAZKFU020000335         3924 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZKFU020000335 DAZKFU020000000
VERSION     DAZKFU020000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.337"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..528
                     /locus_tag="ACHOQQ_RS22625"
                     /old_locus_tag="pgaptmp_004525"
     CDS             <1..528
                     /locus_tag="ACHOQQ_RS22625"
                     /old_locus_tag="pgaptmp_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4472682.1"
                     /translation="EEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLG
                     REGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTLY
                     DLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDH
                     PKLQGIAQQNSFKHT"
     gene            684..2219
                     /gene="gadC"
                     /locus_tag="ACHOQQ_RS22630"
                     /old_locus_tag="pgaptmp_004526"
     CDS             684..2219
                     /gene="gadC"
                     /locus_tag="ACHOQQ_RS22630"
                     /old_locus_tag="pgaptmp_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGU4472683.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2350..3669
                     /locus_tag="ACHOQQ_RS22635"
                     /old_locus_tag="pgaptmp_004527"
     CDS             2350..3669
                     /locus_tag="ACHOQQ_RS22635"
                     /old_locus_tag="pgaptmp_004527"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGU4472684.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
BASE COUNT          967 a          977 c          955 g         1025 t
ORIGIN      
        1 gaagaagcgc tgccgcagga actggtgttc aacgttgact acctgggtgg tcaaattggt
       61 acttttgcca tcaacttctc ccgcccggcg ggtcaggtaa ttgcacagta ctatgaattc
      121 ctgcgcctcg gtcgtgaagg ctataccaaa gtacagaacg cctcttacca ggttgccgct
      181 tatctggcgg atgaaatcgc caaactgggg ccgtatgagt tcatctgtac gggtcgcccg
      241 gacgaaggca tcccggcggt ttgcttcaaa ctgaaagagg gtgaagatcc gggatacacc
      301 ctgtacgacc tctctgaacg tctgcgtctg cgcggctggc aggttccggc cttcactctc
      361 ggcggtgaag ccaccgacat cgtggtgatg cgcattatgt gtcgtcgcgg cttcgaaatg
      421 gactttgctg aactgttgct ggaagactac aaagcctccc tgaaatatct cagtgatcac
      481 ccgaaactgc agggtattgc ccaacagaac agctttaaac atacctgata acgtttaacg
      541 gtaacggtgt cccgaaacga acccgtttcg ggacaatttc caaagtctgt tcactggcat
      601 tagcaacgga aaatattgtt ctgaatacgc ttcagaacaa aacaggtgcg gttccgacag
      661 gaataccgtt ttagggggat aatatggcta catcagtaca gacaggtaaa gctaagcagc
      721 tcacattact cggctttttt gccataacgg catcgatggt aatggctgtt tatgaatacc
      781 ctaccttcgc aacatcgggc ttttcattag tcttcttcct gctattaggc gggattttat
      841 ggtttattcc cgtgggactt tgtgctgcgg aaatggccac cgtcgacggc tgggaagaag
      901 gtggtgtctt cgcctgggta tcaaatactc tggggccgag atggggattt gcagcgatct
      961 catttggcta tctgcaaatc gccattggtt ttattccgat gctctatttc gtgttagggg
     1021 cactctccta cattctgaaa tggccagcgc tgaatgaaga ccccattacc aaaactattg
     1081 cagcactcat cattctttgg gcgctggcat taacgcagtt tggtggcacg aaatacacgg
     1141 cgcgaattgc taaagttggc ttcttcgccg gtatcctgtt acctgcattt attttgattg
     1201 cattagcggc tatttatctg cactccggtg cccccgttgc tatcgaaatg gattcgaaga
     1261 ccttcttccc tgacttctct aaagtgggca ccctggttgt atttgttgcc ttcattttga
     1321 gttatatggg cgtagaagct tccgcaaccc acgtcaatga aatgagtaac ccagggcgcg
     1381 actatccact ggctatgtta ctgctgatgg tggcggcaat ctgcttaagc tctgttggcg
     1441 gtttgtctat tgcgatggtc attccgggta atgaaatcaa cctctccgca ggggtaatgc
     1501 aaacctttac cgttctgatg tcccatgtgg caccggaaat tgagtggacg gttcgcgtga
     1561 tctccgcact gctgttgctg ggtgttctgg cggaaatcgc ctcctggatt gttggtcctt
     1621 ctcgcgggat gtatgtcaca gcgcagaaaa acctgctgcc agcggcattc gctaaaatga
     1681 acaaaaatgg cgtaccggta acgctggtca tttcacagct ggtgattact tctatcgcgt
     1741 tgatcatcct caccaatacc ggtggcggta acaacatgtc cttcctgatc gcactggcgc
     1801 tgacggtggt gatttatctg tgtgcttatt tcatgctgtt tattggctac attgtgttgg
     1861 ttcttaaaca tcctgactta aaacgcacat ttaatatccc tggtggtaaa ggtgtgaaac
     1921 tggtcgtggc tattgtcggt ctgctgactt caattatggc gtttattgtt tccttcctgc
     1981 cgccggataa tatccagggt gactctaccg atatgtatgt tgaattactg gtcgttagtt
     2041 tcctggtggt acttgccctg ccctttattc tctatgctgt tcatgatcgt aaaggcaaag
     2101 cgaataccgg cgtcactctg gagccaatca acagtcagaa cgcaccaaaa ggtcacttct
     2161 tcctgcaccc gcgtgcacgt tcaccacact atattgtgat gaatgacaag aaacactaac
     2221 catgtctgaa tggttataag acaagggagc gataattcat cgctcccttt ttcgtgcttg
     2281 ctgtcgtatt gacttaaccg ggataagtac gagaatgagc gcacatctgt ttaccggaaa
     2341 ccagcacata tggatatctg ctcccgaaac aagaaattaa cgattagaag accagcgata
     2401 ctggttgcac tggcactttt actgtgtagt tgtaaaagca cgcctccaga gtccatggtg
     2461 acaccaccag cgggttcaaa gccaccagcc acgacgcaac aatcgtcaca accgatgcgt
     2521 ggcatctggc tggccacggt gtctcgcctc gactggccac cggtttcctc ggttaacatt
     2581 agtaacccca ccagccgggc ccgtgtacaa caacaggcga tgatcgacaa attggatcat
     2641 ttgcaacgtc tcggtataaa cacggtcttt ttccaggtca agccggacgg taccgccctg
     2701 tggccttcaa aaattttgcc atggtccgat cttatgaccg gtaagattgg tgaaaatccg
     2761 ggttacgatc cgctgcaatt tatgctcgac gaagcccaca agcgtgggat gaaagtacac
     2821 gcctggttta acccctatcg cgtatcggtt aatacgaagc ccggtactat cagggaactg
     2881 aatagcactc tgtctcaaca accggcgagc gtctatgtgc aacaccgtga ctggatcaga
     2941 acgtctggcg atcgctttgt cctcgacccg ggcatccctg aggttcagga ctggatcaca
     3001 tcaatagtcg cagaagtggt ttcccgctat ccggtagatg gcgtgcagtt tgacgactat
     3061 ttctatactg agtcaccggg ttcacggcta aatgataacg aaacgtaccg caaatacgga
     3121 ggcgcatttg cgtcaaaagc agactggcgg cgcaacaata ctcagcagtt aattgcaaaa
     3181 gtatcgcaca ccattaaaag cattaagccg ggagtcgaat ttggtgttag cccggcaggc
     3241 gtgtggcgta accgatcaca cgatccgctc ggttccgata cccgaggcgc ggcagcctat
     3301 gacgaatcct acgctgacac ccgtcgatgg gtggaacaag gattgctgga ttacattgct
     3361 ccccaaattt actggccgtt ctcacggagt gccgcgcgtt atgacgtgtt ggcaaaatgg
     3421 tgggcggatg tcgttaaacc gaccaggacc cgcctgtata tcggtatcgc cttctataaa
     3481 gtgggtgaac cttcaaagat agagccagac tggatgatta acggcggcgt accggaactg
     3541 aaaaagcagc tcgatcttaa cgatgcggta ccagaaatta gcggcaccat cttgttccgt
     3601 gaggactatc tgaataaacc gcagactcaa caagcggtca gctatctgca aagtcgttgg
     3661 ggcagttaaa ctcccattct tactttgtaa caagccggta cattacccac cggcttgtta
     3721 ccctcttgaa ctatcccact actaatcatg cttactaaag tcaaattaac catgcaatta
     3781 accacactta gttaaacagc cataaatccc ctaatacaaa tgtatgaata tgaaaaaaca
     3841 tacgatattc gctgtcaccg aaaaataatt acgatgggct atactcttct gaagaacagt
     3901 agcgtagatt gaaactgtcc taag
//
LOCUS       DAZKFU020000336         3899 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZKFU020000336 DAZKFU020000000
VERSION     DAZKFU020000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.338"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..1710
                     /gene="dpiB"
                     /locus_tag="ACHOQQ_RS22640"
                     /old_locus_tag="pgaptmp_004528"
     CDS             52..1710
                     /gene="dpiB"
                     /locus_tag="ACHOQQ_RS22640"
                     /old_locus_tag="pgaptmp_004528"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGU4472685.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGHPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1679..2359
                     /gene="dpiA"
                     /locus_tag="ACHOQQ_RS22645"
                     /old_locus_tag="pgaptmp_004529"
                     /gene_synonym="citB"
     CDS             1679..2359
                     /gene="dpiA"
                     /locus_tag="ACHOQQ_RS22645"
                     /old_locus_tag="pgaptmp_004529"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGU4472686.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2399..3784)
                     /gene="dcuC"
                     /locus_tag="ACHOQQ_RS22650"
                     /old_locus_tag="pgaptmp_004530"
     CDS             complement(2399..3784)
                     /gene="dcuC"
                     /locus_tag="ACHOQQ_RS22650"
                     /old_locus_tag="pgaptmp_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4472687.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1059 a          940 c         1010 g          890 t
ORIGIN      
        1 cattttaatt acatccgtca aagaggctcg ggacaacccg caaggaaaac aatgttgcag
       61 cttaacgaga ataaacagtt tgcatttttc caaagactgg catttccgct gcgtatcttt
      121 ttgctgattc tggtgttctc aatatttgtc attgcagccc tggcgcaata ttttacggcc
      181 agttttgagg actatttaac gcttcatgta cgcgacatgg caatgaatca ggcgaaaatt
      241 attgcttcta atgacagtat catcagcgca gtaaaaacgc gtgactacaa acggctggcg
      301 accatcgcta acaaattaca aagagatacc gattttgatt atgtggtgat tggcgaccgg
      361 cactcgatcc gcctttacca tcctaatccg gagaaaattg gtcatcctat gcagttcacc
      421 aaacagggcg cgctggagaa aggggagagc tacttcatta ccgggaaagg gtcaatgggt
      481 atggcgatgc gcgccaaaac gccaatcttt gatgacgatg gaaaagtcat cggcgtggtg
      541 tcgattggct acctggtgag taaaatcgat agctggcggg ctgagttttt attaccgatg
      601 gcaggcgtgt ttgtcgtgct gttagggatt ctgatgttgc tgtcgtggtt cctggccgcg
      661 catatccgtc ggcagatgat gggcatggag ccaaagcaaa tcgcacgcgt ggtccgtcag
      721 caagaggcgc tgtttagttc ggtttatgaa gggctgattg cggtggatcc gcacggttac
      781 attaccgcca tcaatcgtaa cgcaagaaag atgctggggc tgagttcccc tggacggcaa
      841 tggttgggta aacccattgc agaagtggtc aggcctgccg atttctttac cgaacagatt
      901 gatgaaaaac gtcaggatgt ggtggcgaac tttaacggtc tgagcgttat tgccaaccgg
      961 gaagctattc gttcaggtga tgatttgctg ggggccatta tcagctttcg tagtaaagac
     1021 gaaatttcca ccctcaatgc gcaactgacg caaataaaac aatacgtcga gagccttcgt
     1081 acattgcgac acgagcatct caattggatg tcgacgctca atggtctgtt gcagatgaaa
     1141 gagtatgatc gcgtgctggc gatggtgcag ggggagtctc aggcccagca acagcttatt
     1201 gacagcctgc gcgaggcgtt tgccgatcgc caggtggcgg ggctgctttt tggtaaagtg
     1261 cagcgcgccc gcgaactggg gctaaaaatg atcatcgtcc ccggtagcca gctttcgcaa
     1321 ctgccgccag gactggatag caccgagttt gccgccattg ttggcaattt acttgataac
     1381 gccttcgaag ccagcctgcg tagcgatgaa ggaaacaaga tcgttgaatt attcctcagc
     1441 gatgaaggcg atgatgtggt gattgaagtc gccgatcagg gctgcggcgt tccagagtct
     1501 ctacgagaca aaatatttga gcagggggtc agtacgcgtg ctgacgagcc cggtgaacat
     1561 ggcattgggt tgtacttgat tgccagctac gtaacgcgct gcggtggtgt tatcactctc
     1621 gaagataatg atccctgcgg taccttattt tcaatctata ttccgaaagt gaaacctaat
     1681 gacagctcca ttaaccctat tgatcgttga ggacgaaacg ccgctggcag agatgcatgc
     1741 ggaatatatt cgtcacattc ccggattcag tcagatatta ctggcgggaa acctggcgca
     1801 ggcccgaatg atgatcgagc gttttaagcc ggggctaatc ttgctcgata actatcttcc
     1861 tgacggtaga gggattaatt tactgcatga actggtgcag gcgcattatc ccggcgacgt
     1921 ggtgtttacc actgcagcca gcgatatgga aacggtgtct gaagccgtac gttgtggtgt
     1981 atttgattat ctcattaagc caattgccta tgaacggctg gggcaaacgc taacccgttt
     2041 ccgccagcgt aaacatatgc tggaaagtat tgatagcgcc agccagaagc aaattgatga
     2101 gatgtttaat gcttatgctc gcggtgaacc taaggacgag ctaccgaccg gcattgaccc
     2161 cttgacgcta aacgcggtgc gaaaactgtt taaagagccg ggtgtgcaac atacggcaga
     2221 aacggtggcg caggcactga ccatcagccg caccactgcc aggcgttatc ttgaatattg
     2281 cgccagccgc catctgatta ttgctgaaat tgttcacggc aaagttggca gaccgcaacg
     2341 catataccac agtgggtgac atgataaagc cggaggaaac tgcctccggc atgactattt
     2401 acttgcctgt gaccgctgct gccgttcctg gcaccatcag ctctgtagca acgataacaa
     2461 tcaccagccc aacaagcact ggcaccgagg tgcgttttac gacttcaaat ggtgagattt
     2521 tcgccatccc ggcaaccgca acgactacgc cagaaacggg tgacagggta cgaccgaggt
     2581 ttgacgcctg cagcatcggg atagtcaaat acgccgggtt aatgccggaa gagtgcgcca
     2641 gtttcgggat catctcaaca aacgcataaa acggcgcatt gcctgaaccg gtcgtgactg
     2701 ccgccagcat tgtcagaatc accaatacca gcatcaggat gatactcgcc gaaccaaacg
     2761 aggtagcgat agaaatcaga ctttgaataa agccgatggt gctaagcccc tgagcgaata
     2821 ccccagcggc aaccagcagc atcaccacgt ttgcaaatgc atctgccatc ccgcgataag
     2881 ccacttccag accagagaaa actttctggg tattaaagct gcggaggaac tccagaatgg
     2941 aggcaatcag catacaaatc accagaatag tgatgatgtg taattgcgga ccccatttgc
     3001 cgtcaaaaat cagcaccccg atgatcggcg tgaacggcaa aatggcataa aacgcagggg
     3061 cagtggtggt tatttcactg acatctaaca tttcatgaga tatgtgctct tttttatcca
     3121 gataacgttg ccagaagaag tgggcgatcg ccatgccgat aattgcagca attgagatag
     3181 gcagcgtcgt tttgaaggcg aagtcaatca gcgacatttc ggaagcctgc gccgccagca
     3241 ccacatcccc tgaagtcggt gcgagaataa tcgccgccgg ggaggcacaa atggcagcag
     3301 ctgctccacg actgatacca acgtttacca tcaccggaaa cagggttgcc atcagcaaaa
     3361 cacccagacc ggttgcggaa gagacggcca gagacatcag acaggcgaca aaataggcgg
     3421 caatcatcag caggtaaggg gagttaatat actgtaatgg ttttgacgcc agcttgacca
     3481 ccatatcatt cgcgccgata tgggtcatgt aagcggcaaa gccacacagc atcataatca
     3541 tcatgccgag gtcgccgccg cggctcatta gcaatatttt aacgtattca acgatatccg
     3601 tggcgctgta gcctgttgaa gcctggctgg acggtaacac tttgtgcccc ataatggcac
     3661 taataatcag caataacagg ccaccgacaa ataacacgcc agtggcggaa taccctttaa
     3721 tgatgtagcg agctacaccc acaataacca caaccccaat aaggagttca atgaatgtca
     3781 gcataatttt tcctgtctcc aggccccaaa gtaaataata aaaaattcct aaaacttaag
     3841 gaaaaaatat gcccaataaa ttggcggtga atgctgatta aaatcaagaa aaactgcca
//
LOCUS       DAZKFU020000337         3898 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZKFU020000337 DAZKFU020000000
VERSION     DAZKFU020000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.339"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..296)
                     /locus_tag="ACHOQQ_RS22655"
                     /old_locus_tag="pgaptmp_004531"
     CDS             complement(<1..296)
                     /locus_tag="ACHOQQ_RS22655"
                     /old_locus_tag="pgaptmp_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel"
                     /protein_id="HGU4472688.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIA"
     gene            complement(597..893)
                     /gene="yciI"
                     /locus_tag="ACHOQQ_RS22660"
                     /old_locus_tag="pgaptmp_004532"
     CDS             complement(597..893)
                     /gene="yciI"
                     /locus_tag="ACHOQQ_RS22660"
                     /old_locus_tag="pgaptmp_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGU4472689.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1117..1836
                     /gene="tonB"
                     /locus_tag="ACHOQQ_RS22665"
                     /old_locus_tag="pgaptmp_004533"
     CDS             1117..1836
                     /gene="tonB"
                     /locus_tag="ACHOQQ_RS22665"
                     /old_locus_tag="pgaptmp_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGU4472690.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTASARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(1876..2274)
                     /gene="yciA"
                     /locus_tag="ACHOQQ_RS22670"
                     /old_locus_tag="pgaptmp_004534"
     CDS             complement(1876..2274)
                     /gene="yciA"
                     /locus_tag="ACHOQQ_RS22670"
                     /old_locus_tag="pgaptmp_004534"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGU4472691.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(2379..2918)
                     /gene="yciB"
                     /locus_tag="ACHOQQ_RS22675"
                     /old_locus_tag="pgaptmp_004535"
     CDS             complement(2379..2918)
                     /gene="yciB"
                     /locus_tag="ACHOQQ_RS22675"
                     /old_locus_tag="pgaptmp_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGU4472692.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2948..3691)
                     /gene="yciC"
                     /locus_tag="ACHOQQ_RS22680"
                     /old_locus_tag="pgaptmp_004536"
     CDS             complement(2948..3691)
                     /gene="yciC"
                     /locus_tag="ACHOQQ_RS22680"
                     /old_locus_tag="pgaptmp_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGU4472693.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(3719..3808)
                     /locus_tag="ACHOQQ_RS22685"
                     /old_locus_tag="pgaptmp_004537"
                     /pseudo
     CDS             complement(3719..3808)
                     /locus_tag="ACHOQQ_RS22685"
                     /old_locus_tag="pgaptmp_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT         1143 a          939 c          917 g          899 t
ORIGIN      
        1 gctatcaaca acaaaatgat actgattgcc catgcgagct ttgcccggaa cagtaagcca
       61 atggagttca ggacaagaaa gacgccgagc ataaatagtg gggcgtttgc cagcaaggag
      121 agatccaact ctgaaaatgc tttgatatgg aaaatatcaa gcaaattgac cgacatacca
      181 tagattgttt taaaaataag caatccattt aaaaagacgg ccagcgcgag aatgtcgtgc
      241 cgtagggtca cccataaatt tgttgctgtt tgtttgaatg tagcccagtg actcactgat
      301 acctctgtaa ccacaatttt gtttaattat cttagcgtgc acattttctg aacatacatg
      361 cagcgcgtga atgtgttaaa caatagtaga tgactttatc cgcagtaatc aaaggttttg
      421 agtgagcaac tctgcgtcag gaaagggcat ttttcctgaa tatgctcaca tcatataaag
      481 aaatacagat aaagttatta tctgcttgtg gtggtgaatg cactgaccga cctataagga
      541 aaggccaaac aagaacacgg ttgcaaaaac cgtgccctta aatattggct ctctactcag
      601 aacactttct taaatggttt cactgaaacg tgttcataga ctcctgccgc tacgtacggg
      661 tcagcatcgg cccaggcctg agctgcttcc agcgattcaa attcagcaat aacggttgag
      721 ccagtaaatc ccgcagcccc tggatcgtta ctgtctaccg ctggcattgg accagctgtc
      781 aacaaacgac cttcatcatg cagtaactgt aaacgtgcta aatgtgccgg acgaacggaa
      841 aggcgttttt cgaggctatc agctttgtct tgagcgtaaa taacatacaa cacgggcaca
      901 actccttgtt cgggaaagtt gtaaagtacg ttatttgaaa gggcgaagat ctgcaacgga
      961 aagatgatgt ctttgttaag accatgcata aagtaaggtc aattacgcca aaaatgacat
     1021 tttcactgat cctgatcgtc ttgccttatt gaatatgatt gctatttgca tttaaaatcg
     1081 aggcctggtt tttctactga aatgattatg acttcaatga cccttgattt acctcgccgc
     1141 ttcccctggc cgacgttact ttcggtctgc atacatggtg ctgttgtggc gggtctgctc
     1201 tatacctcgg tacatcaggt tattgaacta cctgcgcctg cgcagccaat ttctgtcacg
     1261 atggttgcgc ctgctgatct cgaaccgcca caagccgttc agccgccacc ggagccggtg
     1321 gtagagccag aaccggaacc tgagccgatc cccgaaccgc caaaagaagc accggtggtc
     1381 attgaaaagc cgaagccgaa acctaagcca aaaccgaagc cggtgaaaaa ggtacaggag
     1441 cagccaaaac gcgatgtcaa acccgtagag tcgcgtccgg catcaccgtt tgaaaatacg
     1501 gcatcggcac gcccgacatc aagtacggca acggctgcaa ccagcaagcc ggttaccagt
     1561 gtggcttcag gaccacgcgc attaagccgt aatcagccgc agtatccggc acgagcacag
     1621 gcattgcgca ttgaagggca ggttaaagtt aaatttgatg tcacgccgga tggtcgcgtg
     1681 gataacgtgc aaatcctctc agctaaacct gcgaacatgt ttgagcgtga ggtgaaaaat
     1741 gcgatgcgca gatggcgtta tgagccgggt aagccaggca gtgggattgt ggtgaatatc
     1801 ctgtttaaaa ttaacggcac caccgaaatt cagtaagcag aaagtcaaaa gcctccgacc
     1861 ggaggctttt gattattact caacaggtaa ggcgcgaggt tttccttcag gatcaaccgc
     1921 gacatactta aataatgctt ctgtcgcttt atagcgttgc ccgattggtt cagacgctac
     1981 ttttttcacc cacacttcaa tattaatgct gaccgatgtc gtccctttct ggacacagcg
     2041 tgcatagcag cacaccacat cgccgaccgc aaccggccgt aagaaagtca ttccttcaac
     2101 ccgcacagtc actacgcgac cgtgggcaat ttctttcgcc agaatagcgc cgccaatatc
     2161 catttgtgac attaaccaac caccaaagat gtcaccattg gcattggtat cggcgggcat
     2221 ggctaaagta cgtaaaacaa gatcgccctg agggacgtta tgtgttgtag acatggtaaa
     2281 accgacttaa aaggaaaatc acaggcgcga tgctactatg attttcctga ggagaacaga
     2341 ggagatacag cgccagcccc gaaggactgg cagtctggtt aggatttatc ttcctgcggc
     2401 atgtggcggt agatatagat accgcttaac aatgtgaaga ttaaggtaag ggcggtcagg
     2461 ccaaagactt taaagttgac ccaaatattt tgcggcagcc agaatgcgat gtagatgttt
     2521 gccagaccgc aaaggataaa gaaaacagcc caggccagat tcagcttcga ccataccggt
     2581 tgcggcagcg tgagttcttt acccagcatc cgctgaatta gcggcttttt catcacccat
     2641 tggctgacta acagggcacc cgcaaacagg gcataaatga cagtaacctt ccatttaata
     2701 aactcatcat tgtggaagaa cagcgtcaag ccaccgaaga cgaccaccag aacaaaagtg
     2761 atcagggcca tcttctcaac cttacgaaag cgaacccagc tatagataag cacaatcgcc
     2821 gtggcgacga tcagcgccgc agtagccgca tagatgtcat aaatcttgta aaacgcgaaa
     2881 aagacaacca gcggtaaaaa atcaagaaac tgcttcattt tacgattccg taatcaaatg
     2941 cttaaggtta ttggcgaatc aacatataca gacggaacag atagatgagc aatacggctg
     3001 aaatcaggtt gctcaaggtg ttcgccagta cggcaccaat ttccggggtt aatgcggcaa
     3061 aagaagaggc aaaaagcagc aacagtgtct ttgccagcaa ccagctcagt actgcgggcg
     3121 ccaccagacg catattcgcc caagtcagcc gcatactgct acgcatcgag gcaaaaacgc
     3181 ccattttgtc ctgtaccaac atcaccggtg ccagggccag taaaatggcc atgataattc
     3241 ccggaacgac cactagcatg atgccaatct gtactaaaag ggtagtcaga aaaatcagaa
     3301 taaataactt tggcaatatc ggcgcactgg caccaatagc tcgcagcgca ctgactctct
     3361 gacccgcaga caccagctgg ataattaata ttacgcctcc ggcgagaatg gcgttaccga
     3421 ttaatcctga aaaagtggac gccgctgaag cctgcagcaa aatttgttgc tgttccggtg
     3481 acatattctg aaccaggtcg aacaacccac tactgccgct aacgggcacg ccgtcattga
     3541 gctgcgcaag ctgtgcatca ctgggtgaga aaacatgccc taacaccact gtgataaacg
     3601 cacatagcaa cgataccaac agaatggtca taaattgatt acggaagaaa tttccggtgt
     3661 cacggtatac ggactgcgcc gtgatagaca tgcactctcc ttgagtttta tgcaggtgtt
     3721 aattagcggg caattgtacc ctggttaagc ctcgggtggc agcatcaggt cttgtatgga
     3781 aaagcatatc tttgtaaagc ggaggtaatt gctggccttc aggcgtgggc aaaccgcggc
     3841 aagtggcgat cgagttaata tttgcgtagc gaaaatattt aaaaattgat ttaaatca
//
LOCUS       DAZKFU020000338         3841 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZKFU020000338 DAZKFU020000000
VERSION     DAZKFU020000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.340"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..335)
                     /locus_tag="ACHOQQ_RS22690"
                     /old_locus_tag="pgaptmp_004538"
     CDS             complement(<1..335)
                     /locus_tag="ACHOQQ_RS22690"
                     /old_locus_tag="pgaptmp_004538"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4472694.1"
                     /translation="MSELSYLEKLLDGVEVEWLPLGEVSALRRGRVMSKGYLTENFGP
                     YPVYSSQTANNGKIGSINTFDFDGEYISWTTDGANAGTVFYRTGKFSITNVCGLITLK
                     SKYPLIYKFL"
     gene            complement(332..1888)
                     /locus_tag="ACHOQQ_RS22695"
                     /old_locus_tag="pgaptmp_004539"
     CDS             complement(332..1888)
                     /locus_tag="ACHOQQ_RS22695"
                     /old_locus_tag="pgaptmp_004539"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HGU4472695.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSGYMEAGDDSICYAALDDGIITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLNLGDFNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWTGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSARGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTRVQFIDASELFKKEANNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCGVQK"
     gene            2154..3026
                     /locus_tag="ACHOQQ_RS22700"
                     /old_locus_tag="pgaptmp_004540"
     CDS             2154..3026
                     /locus_tag="ACHOQQ_RS22700"
                     /old_locus_tag="pgaptmp_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4472696.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            3359..>3841
                     /locus_tag="ACHOQQ_RS22705"
                     /old_locus_tag="pgaptmp_004541"
     CDS             3359..>3841
                     /locus_tag="ACHOQQ_RS22705"
                     /old_locus_tag="pgaptmp_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HGU4472697.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     G"
BASE COUNT          930 a          967 c          952 g          992 t
ORIGIN      
        1 agaaatttgt agattaatgg atatttactt tttaatgtaa ttaggccgca aacattagtt
       61 attgaaaatt tacctgttcg atagaacaca gtgcctgcat ttgcgccatc tgttgtccag
      121 cttatatatt cgccatcaaa atcgaaggta ttgatagaac ctattttccc attatttgca
      181 gtttgagagc tatagacagg atatgggcca aaattttcag ttaaatatcc ctttgacata
      241 actctgcctc ttcttaaggc tgagacttcc cccaatggca accactcaac ctcaacccca
      301 tccagcaatt tttccagata actcaactcg ctcatttctg caccccgcag ccttcaattt
      361 cagccacaat cgcatcaata tctttacgca actggtcgat tttgctgacc gtggttttca
      421 gctcagcatt cagctcagca atattgataa tttcacgagt atctttcgct tccacatagc
      481 tgctcaccga caggttatag tcattagcga caacggtctc aaacgcgact gatttcgcca
      541 gatgggcaac atcttccttg ctggcaaata cctgcataat ctgttcgata tgagcatcgg
      601 tcagaatgtt gttgttagcc tcttttttga acagttcgct ggcatcaata aactgaactc
      661 tggtatccgt tttatgttta gacagcacca gtatgtttac ggcaatggtg gtgccaaaga
      721 acaggttcgg tgccagtgaa atcacggttt cgacatagtt attgtcgacc agatactgac
      781 ggattttctg ctctgcgccg ccacggtaaa aaatacccgg gaagcagaca atcgcagcgc
      841 gacctctggc cgaaagatag ttcagcgcat gtaatacaaa cgcaaagtca gctttggatt
      901 tgggggccag aacgccagcc ggggcaaaac gctcatcgtt aatcagtgtc gggtcatcgc
      961 tgccagtcca tttcaccgaa tacggcgggt tagaaacgat ggcatcaaac ggtttttcat
     1021 ctctgaagtg cggctcagtc agcgtattgc ccagcttgat atcaaacttg tcgtagttga
     1081 tgttgtgcaa aaacatgttc atacgcgcca ggttataggt cgtatggttg atttcctgac
     1141 caaaaaagcc ttcttcgatg atatggtcat caaactgctt tttcgcctgc aacaacagcg
     1201 aaccggaacc cgctgccggg tcgtagattt tgttaacgtg ggtctgcccg tgcatagcca
     1261 gttgtgcaat cagcttagag acgtgctgtg gtgtaaagaa ctcgccgcct gacttaccgg
     1321 catttgcggc atagttagaa ataaggaatt cataggcatc gccaaacagg tcaatctgat
     1381 gtttgttgaa gtcaccaagg tttaaccctt caaccccttt cagaacagca gccaggcggg
     1441 catttttatc cttaacggtg ttacccaggc ggttactggt ggtatcgaaa tcagcaaaca
     1501 aacccttgat gtcagcttct gaaggataac cgtaagcaga actttcgata gcaacgaaga
     1561 tgctgtttaa atctgcattc agtctgtcat tggtatttgc tttcgcagct acgttgcaga
     1621 aaagctgact ggggtagatg aagtagcctt tagtcctgat ggcatcgtct ttaatgtcat
     1681 cagtaataat gccgtcatca agtgccgcat aacagatgct gtcatcaccg gcttccatat
     1741 acccggagaa attttcgctg ataaaacggt agaaaagcgc gcccagaacg tattgcttaa
     1801 aatcccatcc atcgaccgaa cctctgacat cgttagcaat ttgccatatc tggcggtgca
     1861 gttctgctct ttgttgaata cttgtcattt ttaacctgtt tttcctggtc tggaactgaa
     1921 tctccggggg caatactaca gcaatcttgt ggttcaggca gccccggagc aagtcagcag
     1981 atattgtgag atacaccagg ttattcatga cagggaggag gaacggaaca ctgcctttct
     2041 tttacagtgc accgggtaca ttcaaaataa tttcagacag atattatcgt tgtgagtccc
     2101 ccgccacctt tccggtgcgc ggggttttgt cttttttcac cgggaataca tgtatgaatc
     2161 cgtctgatgc cattgaggca attgaaaaac cgctctcctc cctgccttac tcactttccc
     2221 gtcacatcct ggaacatctg cgcaaactca cccgtcacga acccgtgatt ggcattatgg
     2281 gtaaaagcgg ggccggtaaa tcctcactct gtaatgcact gtttcagggg gaggtcaccc
     2341 cggtcagtga tgttcacgcc ggcacccggg aagtacggcg cttccgtctg agtggccacg
     2401 gtcacagcat ggttatcact gacctgcccg gggtgggcga gagccgggac agggatgcag
     2461 agtatgaggc cctgtaccgt gacattctgc ctgaacttga cctggtgctg tggctgatta
     2521 aagccgatga ccgggccctg tctgtggatg agtatttctg gcgacacatc ctgcagtgcg
     2581 gacatcagca ggtgctgttt gtggtgacgc aggccgacaa aacggagccc tgccatgaat
     2641 gggatatggc cggcattcag ccttctcccg cacaggaaca gaacattcgt gagaaaacag
     2701 atgcggtatt ccggctgttc cggcccgtac atccggttgt ggccgtatcg gcccgcaccg
     2761 gctgggaact ggatacgctg gtcagtgcgc tcatgacagc acttcccgac catgccgcca
     2821 gtcccctgat gacccgcctg caggacgagc tgcgcacgga gtctgtccgc tcgcaggccc
     2881 gtgaacagtt taccggtgcg gtggaccgga tatttgacac agcggagagc gtctgtgttg
     2941 cctctgttgt ccgtacggcc ctgcgcgctg ttcgtgacac cgtggtctct gttgcccgcg
     3001 cggtatggaa ctggatcttc ttctgaacct gttgtggatg atgtcctccc tgcctctgag
     3061 tctgctcaca aaagcgctgt tttcgttact gtctctcttg tccgtgcaat agccaaataa
     3121 tagaataaaa cgatcgatac ctattttatc gatcgtttat atcgatcgat aagctaataa
     3181 taacctttgt cagtaacatg ctcagatacg tacagaaaga cattcaggga acaacagagc
     3241 cacacgtcag aaacttccgt cagccggacc tctggcactg taacccttta cctgccggta
     3301 tccacatctg tggataccgg cttttttatt caccctcact ctgattaagg aaatgctgat
     3361 gaaacgacat ctgaatacct gctacaggct ggtatggaat cacattacgg gcgctttcgt
     3421 ggttgcctcc gaactggccc gcgcacgggg taaacgtggc ggtgtggcgg ttgcactgtc
     3481 tcttgccgcg gtcacgtcac tcccggtgct ggctgctgac atcgttgtgc acccgggtga
     3541 aacagtgaat ggcggaacac tggtaaacca tgacaaccag tttgtatccg gaacagctga
     3601 tggcgtgact gtcagtaccg ggcttgagct ggggccggac agtgacgaaa acaccggcgg
     3661 gcaatggata aacgcgggtg gcacaggcag aaacaccact gtcaccgcaa atggtcgtca
     3721 gattgtgcag gcaggaggaa ctgccagtga tacggttatt cgtgatggcg gagggcagag
     3781 ccttaacgga ctggcggtga acaccacgct ggataacaga ggtgagcagt gggtacacgg
     3841 g
//
LOCUS       DAZKFU020000339         3788 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZKFU020000339 DAZKFU020000000
VERSION     DAZKFU020000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.341"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1677
                     /locus_tag="ACHOQQ_RS22710"
                     /old_locus_tag="pgaptmp_004542"
     CDS             <1..1677
                     /locus_tag="ACHOQQ_RS22710"
                     /old_locus_tag="pgaptmp_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HGU4472698.1"
                     /translation="IALEVWPGSEFQAETQQTRISLRSLINSIYGIKRTLAKIFCLSV
                     VIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISAGLMFFILLRAAVSMLRAWSSLVMS
                     TLINVQWQSGLFDHLLRLPLAFFERRKLGDIQSRFDSLDTLRATFTTSVIGLIMDSIM
                     VVGVFVMMLLYGGYLTWIVLCFTTIYIFIRLVTYGNYRQISEECLVREARAASYFMET
                     LYGIATVKIQGMVGIRGAYWLNMKIDAINSGIKLTRMDLLFGGINTFVTACDQVVILW
                     LGAGLVIDNQMTIGMFVAFSSFRGQFSERVTSLTSFLLQLKIMSLHNERIADIALHEK
                     EEKKHEIEIVAHMGPISLETNDLSYRYDSQSAPIFSALSLSVAPGESVAITGASGAGK
                     TTLMKVLCGLFEPDSGRVLINGIDIRQMGINNYHRMIACVMQDDRLFSGSIRENICGF
                     AEEMDEEWMVECARASHIHDVIMNMPMGYETLIGELGEGLSGGQKQRIFIARALYRKP
                     GILFMDEATSALDSESEHFVNVAIKNMNITRVIIAHRETTLKTVDRVISI"
     gene            complement(1864..2181)
                     /locus_tag="ACHOQQ_RS22715"
                     /old_locus_tag="pgaptmp_004543"
     CDS             complement(1864..2181)
                     /locus_tag="ACHOQQ_RS22715"
                     /old_locus_tag="pgaptmp_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V family bacteriocin"
                     /protein_id="HGU4472699.1"
                     /translation="MREITLNEMNNVSGAGDVNWVDVGKTVATNGAGVIGGAFGAGLC
                     GPVCAGAFAVGSSAAVAALYDAAGNSNSAKQKPEGLPPEAWNYAEGRMCNWSPNNLSD
                     VCL"
     gene            complement(2398..2634)
                     /gene="cvi"
                     /locus_tag="ACHOQQ_RS22720"
                     /old_locus_tag="pgaptmp_004544"
     CDS             complement(2398..2634)
                     /gene="cvi"
                     /locus_tag="ACHOQQ_RS22720"
                     /old_locus_tag="pgaptmp_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V immunity protein"
                     /protein_id="HGU4472700.1"
                     /translation="MDRKRTKLELLFAFIINATAIYIALAIYDCVFREKDFLSMHTFC
                     FSALMSAICYFVGDNYYSISDKIKRRSYENSDSK"
     gene            3226..3372
                     /locus_tag="ACHOQQ_RS22725"
                     /old_locus_tag="pgaptmp_004545"
                     /pseudo
     CDS             3226..3372
                     /locus_tag="ACHOQQ_RS22725"
                     /old_locus_tag="pgaptmp_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(3422..3775)
                     /gene="cmi"
                     /locus_tag="ACHOQQ_RS22730"
                     /old_locus_tag="pgaptmp_004546"
     CDS             complement(3422..3775)
                     /gene="cmi"
                     /locus_tag="ACHOQQ_RS22730"
                     /old_locus_tag="pgaptmp_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HGU4472701.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQFSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
BASE COUNT         1119 a          769 c          785 g         1115 t
ORIGIN      
        1 attgcacttg aggtctggcc cggaagtgaa ttccaggcgg aaacccagca gacccgcata
       61 agtctccgtt cactgattaa cagtatttac ggtattaaaa gaacactggc gaaaattttc
      121 tgtctgtcag ttgtaattga agcaatcaat ctggtaatgc cggtgggaac tcagctggtt
      181 atggatcatg cgattccggc gggggacaga gggctgctaa cgcttatttc tgctggcctg
      241 atgttcttta tattgctcag ggccgcggtg agtatgctgc gtgcatggtc ctcactggtt
      301 atgagcacgc tcatcaatgt acagtggcag tcggggctgt tcgatcatct tctcagacta
      361 ccgctggcgt tttttgaacg ccgaaaatta ggtgatatcc agtcacgttt tgactccctt
      421 gacacattga gggccacatt taccaccagt gtgatcgggt taataatgga cagcattatg
      481 gttgtcggtg tttttgtgat gatgctgtta tacggaggat atctcacctg gatagttctc
      541 tgctttacca caatttacat ttttattcgt ctggtgacat acggcaatta ccgacagata
      601 tcagaagaat gtcttgtcag ggaggcccgt gccgcctcct attttatgga aacattatat
      661 ggtattgcca cggtaaaaat ccaggggatg gtcggaattc ggggagcata ctggcttaat
      721 atgaaaatag atgcgataaa ttcgggtatt aagctaacca ggatggattt gctcttcgga
      781 ggaataaata cctttgttac cgcctgtgat caggttgtaa ttttatggct gggagctggc
      841 cttgtgatcg ataatcagat gacaatagga atgtttgtag cgtttagttc ttttcgtggg
      901 cagttttcgg aaagagttac ctctctgacc agttttcttc ttcagctaaa aataatgagt
      961 ttacacaatg agcgcattgc agatattgca ttacatgaaa aggaggaaaa gaaacatgaa
     1021 attgaaatcg ttgctcatat ggggccaata tccctggaaa ccaatgattt aagctaccgg
     1081 tatgacagtc agtcagctcc gatattcagt gctctgagtt tatcagtagc tccgggggaa
     1141 agtgtggcta taactggtgc ttccggtgcg ggaaaaacca cattaatgaa agtactatgt
     1201 ggactatttg aacctgatag cgggagggta ctgataaatg gtatagatat acgccaaatg
     1261 ggaataaata attatcaccg gatgatagcc tgtgttatgc aggatgaccg gctattttca
     1321 ggctcaattc gtgaaaatat ctgtggtttt gcagaggaaa tggatgaaga gtggatggta
     1381 gaatgtgcca gagcaagtca tattcatgat gttataatga atatgccaat gggatatgaa
     1441 acattaatag gtgaacttgg ggaaggtctt tctggcggtc aaaaacagcg tatatttatt
     1501 gcacgagcct tataccggaa accaggaata ttatttatgg atgaggcaac cagtgctctt
     1561 gattcagaga gtgaacattt cgtgaatgtt gccataaaaa acatgaatat aaccagggta
     1621 attattgcac acagagaaac aacgttgaaa actgttgaca gagttatttc catttaaaac
     1681 ctactgacat aaccatagag gaattgcaac tgtatgagga atatatcttc ctgtgataat
     1741 tcctcgttat gttcagttgt cagttggagg aggaatggaa gatagacaac ccaccaggaa
     1801 gcatatgtat cattctgtgt ggattgtcca ataacagttc tttcctatat taaaaaatac
     1861 tatttataaa caaacatcac taagattatt tggactccaa ttacacattc taccttcagc
     1921 gtagttccat gcttctggag gtagtccttc tggtttttgt ttcgctgagt tggaatttcc
     1981 tgctgcatca tacaaagcag caacagcggc agaagatcca acagcgaagg caccagcaca
     2041 aacagggccg cacagaccag ctccgaatgc accgccaatc actcctgcac cgtttgttgc
     2101 tacagttttc ccgacatcaa cccaattgac atcaccagca ccagagacat tattcatttc
     2161 atttaacgtt atttctctca tatatcattt acccctatta aaaaaaatag atatcattaa
     2221 tgcaatggcg ataccgctaa caacagattg aacaaggttg ctagaattta gttgtttagc
     2281 aataagacta ataataatgc ttatgggtaa tataataaaa aatacttgcc acgttttcat
     2341 ctatattcct gcgctaaagt tatagtaacg ccaacacctc cagaaacaga atctaattca
     2401 tttagagtca gagttctcat atgatctcct ttttatctta tcggatattg aataataatt
     2461 atcaccaaca aagtaacata ttgcagacat taatgcagag aagcaaaatg tatgcatgga
     2521 taaaaaatcc ttttctctaa aaacacaatc atatatagct aatgcaatat atattgcggt
     2581 ggcatttatt ataaatgcaa ataacaactc taattttgtt ctttttctat ccattacttt
     2641 ctatcccatt accatacaaa cactaacgat aatgattatc gtttacatag tcaagagtga
     2701 agggtagggg agccctcacc cccctataag gggtccgcct ggaaaacgga gtttcccacg
     2761 tcaagagaat tgagttgaac aagtggttac atccatccaa gccagctaac tcactaaacg
     2821 taaaggatgt acgaaatgtt cctaccgtcc gataaccatt tggcttactt ccgtacattc
     2881 ccttgcgtag atgttcagaa ttgtacgagt tcggacacca aaaccgctgt tgtcccgcca
     2941 ttctggcgat tcctgaaagt catttgacta aaaaataggg gctatttcga attttctgga
     3001 cactcctgac gcggagtctg tgacttcaac ctgacacctt acgctcttag cgtacgattt
     3061 tttccgtttc gtgagttgac ccctttattt tccaaattcc aaactgcccc ctacatttat
     3121 acaatgtccg gcagggcata gaaggggaga aagaaaatac ctgggcgaag ccgctgaaca
     3181 tcattctggc gatttcaata aaggcatttg ctcgcgctct gtcattatga tgataaatac
     3241 gattttaacg ccagcactca ccgtggcgtc atcggagagt cgctcacaag gctcggggcg
     3301 atgtgttctg gtaaagagta ccagatactg cttcctggtg aaattcacat taaagaaagt
     3361 ggtaagcgat aatataaggg ccgggattcc ggccttttta tgcagccttt ataacagtgt
     3421 attagtcatt cgcatattca acatatccct cctcacaatg gtacatgcca taatacattc
     3481 tcttaccctc cggaccttca gctttaaatg ggacaagaag tgttttttca tcttcaacat
     3541 ctgttaaact tctttcaatc ttttcccatg taattttcgg gatttctgta ccaagaagtt
     3601 gtacatcgtt gtcccagcgt ttcattcttt gcaggtaatc attttttaca aatgttctgg
     3661 cctgttcatc gctgaactga tagcatgccg gacctttatc ttcagaccag aaaaaaacag
     3721 cagcaagagc aataatcgtt aaaataaaaa taaatttcat gctaattact ttcataaaat
     3781 ctccacaa
//
LOCUS       DAZKFU020000340         3752 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZKFU020000340 DAZKFU020000000
VERSION     DAZKFU020000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.342"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            274..534
                     /locus_tag="ACHOQQ_RS22735"
                     /old_locus_tag="pgaptmp_004547"
     CDS             274..534
                     /locus_tag="ACHOQQ_RS22735"
                     /old_locus_tag="pgaptmp_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HGU4472702.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            758..832
                     /gene="tap"
                     /locus_tag="ACHOQQ_RS22740"
                     /old_locus_tag="pgaptmp_004548"
     CDS             758..832
                     /gene="tap"
                     /locus_tag="ACHOQQ_RS22740"
                     /old_locus_tag="pgaptmp_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGU4472703.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            825..1682
                     /gene="repA"
                     /locus_tag="ACHOQQ_RS22745"
                     /old_locus_tag="pgaptmp_004549"
     CDS             825..1682
                     /gene="repA"
                     /locus_tag="ACHOQQ_RS22745"
                     /old_locus_tag="pgaptmp_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGU4472704.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            2382..2575
                     /locus_tag="ACHOQQ_RS22750"
                     /old_locus_tag="pgaptmp_004550"
                     /pseudo
     CDS             2382..2575
                     /locus_tag="ACHOQQ_RS22750"
                     /old_locus_tag="pgaptmp_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2617..2946
                     /locus_tag="ACHOQQ_RS22755"
                     /old_locus_tag="pgaptmp_004551"
     CDS             2617..2946
                     /locus_tag="ACHOQQ_RS22755"
                     /old_locus_tag="pgaptmp_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4472705.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            2927..3208
                     /locus_tag="ACHOQQ_RS22760"
                     /old_locus_tag="pgaptmp_004552"
     CDS             2927..3208
                     /locus_tag="ACHOQQ_RS22760"
                     /old_locus_tag="pgaptmp_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4472706.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
     gene            3391..3705
                     /locus_tag="ACHOQQ_RS22765"
                     /old_locus_tag="pgaptmp_004553"
     CDS             3391..3705
                     /locus_tag="ACHOQQ_RS22765"
                     /old_locus_tag="pgaptmp_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HGU4472707.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNPLGVLRPFPPKVSL
                     LA"
BASE COUNT         1013 a          951 c          934 g          854 t
ORIGIN      
        1 ggggacggaa gtccccgctt tccggaagtg tgaggtattt caggggcaga cacccgacat
       61 gccagaaacc gccggtcccg cccggggccg gcacacaggt taaggcattt cctgtttttc
      121 agccatttcg caacaaaaat cacattaaac aacagtaatc accgttgatt tgtgcgccaa
      181 cacagatctt cgtcacaatt ctcaagtcgc tgatttcaaa aaactgtagt atcctctgcg
      241 aaacgatccc tgtttgagaa ttgaggaggc gagatgtcgc agacagaaaa tgcagtgact
      301 tcctcattga gtcaaaagcg gtttgtgcgc agaggtaagc ctatgactga ctctgagaaa
      361 caaatggccg ctgttgcaag aaaacgtctt acacacaaag agataaaagt ttttgtcaaa
      421 aatcctctga aagatctcat ggttgagtac tgcgagagag aggggataac acaggctcag
      481 ttcgttgaga aaatcatcaa agatgaactg cagagactgg atatactaaa gtaaagactt
      541 tactttgtgg cgtagcatgc tagattactg atcgtttaag gaattttgtg gctggccacg
      601 ccgtaaggtg gcagggaact ggttctgatg tggatttaca ggagccagaa aagtgaaaac
      661 cccgataatc ttcgacaagt ttggcgactg agaagattac cggggccatc taaaaaccgt
      721 atagccaaca attcagctat gcggggagta tagttatatg cccggaaaag ttcaagactt
      781 ctttctgtgc tcgctccttc tgcgcattgt aagtgcagga tggtgtgact gatcttcaac
      841 aaacgtatta ccgccaggta aagaacccga atccggtgtt tacaccccgt aaaggtgccg
      901 gaacgctgaa gttctgcgaa aaactgatgg aaaaggcggt gggcttcacc tcccgttttg
      961 atttcgccat tcatgtggcg catgcccgtt cgcgtggtct gcgtcgacgc atgccaccgg
     1021 tgctgcgtcg acgggctatt gatgcgctgc tgcaggggct gtgtttccat tatgacccgc
     1081 tggccaaccg cgtccagtgt tccattacca cactggccat tgagtgcgga ctggcgacag
     1141 agtccggtgc aggaaaactc tccatcaccc gggccacccg ggccctgacg ttcctgtcag
     1201 agctgggact gattacctac cagacggaat atgacccgct tatcgggtgc tacattccga
     1261 ccgatatcac gttcacaccg gcgctgtttg ccgcccttga tgtgtctgag gatgcagtgg
     1321 cagctgcgcg ccgcagccgt gtggtatggg aaaacagaca gcgcaaaaag caggggctgg
     1381 ataccctggg tatggatgag ctgatagcga aagcctggcg ttttgtccgt gagcgtttcc
     1441 gcagttacca gacagagctt aagtcccacg gcatgaaacg tgcccgtgcg cgccgcgatg
     1501 caggcagaac ccgccaggac attgtcaccc tggtgaaacg gcagctgacg cgtgaaatcg
     1561 cggaagggcg tttccgggga agcctggagg ccgtgaagcg cgaaatcgac cgtcgtgtga
     1621 aggaacgcat gataatgtcg cggaacaaca actacacacg gctggccact gcctccccct
     1681 gagttttacc tcatctgaat aatccggcct gcgccggagg catctgcacg cctgaagcct
     1741 gtcagcgaac aaaaaaacag caccacatac aaaaaacaat ctcatcatcc cgcttctggt
     1801 gcatccggcc cccctgtttt cgatacaaaa catgcctcac agacgtggaa ttttgcttat
     1861 ccacatttaa ctgcaaggga cttccccata aggttacaac cgttcatgtc ataaagcgcc
     1921 agccgtcagc gttacagggt gcaatgtatc ttttaaacac ctgtttatat ctcctttaaa
     1981 ctacttaatt acattcattt aaaaagaaaa cctattcatt gcctgtcctg tggacagaca
     2041 gatatgcacc tcccaccgca agcggcgggc cccgaccgga gccattttag ttacaacact
     2101 caaatacaag caccgggaaa accccggtcc agcgcagaac cgaaaccgca aagcccctcc
     2161 ctcataactt aaaagcggcc ccgccccggc ccaaagggcc ggaacagagt cgcttttaat
     2221 tatgaatgtt gtaactacat tatcatcgct gtcagtcttc tggctggaag tcctcagtac
     2281 acgctcgtaa gcggccctga cggcccgcta acgcggagat acgccccgac ttcgggtaaa
     2341 ccctcgtcgg gaccactccg accgcgcaca taagctctat catagctgac agcgggtatg
     2401 gctttgcagg gtggggatgg gtaaggtgaa atctatcaat cagtaccggc ttacgccggg
     2461 cttcggcggt tttactccgg tatcacatgt aaccacagcg tgccgcctta catgcgctga
     2521 cgcgcataaa caacaagttg ctacattagc ttacaggtta attcaccttg cataagatta
     2581 gcatataggt taatgtttat cgaaatggga ggtcatatgt ttagcgttgt ttatcacccg
     2641 gaagccaggg aagaagccac agcattaccc ataaaaatca gggtgaagtt tgaccgtctc
     2701 atcagcaaac tggaatatga cgcccgatta ctgcgggaac cggatacaaa gcccctgggg
     2761 gatggacttt ttgagatccg cacgatgggg acggacatag ccagaggcat ctgggtatac
     2821 cacaaaggca ataccatcat catgctccgg gttttcatca agaagtctca gaaaacgcca
     2881 gcaaaagaaa tcgatctcgc caaaaagcgg ttagcggagg tacttaatga aactggtaag
     2941 ccataaagaa ctgcataacg agtggatgca ggacgacgaa tatcgcgctg catggcagga
     3001 agaagaacgt aaggagaaac tcagggagtt gcttgcagaa tggcgtaagc atgacaattt
     3061 gacaaaagcg caggttgctg aacgcatggg ggttactcct cctgttattt cccgcctgga
     3121 aaacaacatc actaaagcca gcatcgacac cctgactcgc tatgcccatg cgtgcggtat
     3181 caaaaaacct gttattacgc tgtactgaac tctgctctat ggctgccagt tctggagaaa
     3241 tcagtaacta aattttaatt agctacgtta tggtaaatct gcctgatgtg tcgatttctc
     3301 tgaaagtaat tcggcttctg tgggctgcgt ttgccttgtg ccacctgaat gtaatcacta
     3361 tgattaaata tgattaacaa ctaatcggat atacaaatga aaaacaatac cgcacaggca
     3421 acaaaagtaa ttaccgcgca tgtgccatta cctatggctg ataaagtcga ccagatggcc
     3481 gccagactgg aacgctcccg gggctgggtt atcaaacagg cactttctgc atggcttgcc
     3541 caggaggagg agcgtaatcg cctgacgctg gaagccctgg acgatgtgat atccggacag
     3601 attatcgacc atcaggctgt acaggcctgg gcagacagcc tcagtactga caatccgtta
     3661 ggggttctgc gcccatttcc tccaaaagtc tcactcttag cataaaaacc attatttatc
     3721 atgttgttaa ccgtcatatt cggactcaat at
//
LOCUS       DAZKFU020000341         3734 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZKFU020000341 DAZKFU020000000
VERSION     DAZKFU020000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.343"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(95..661)
                     /locus_tag="ACHOQQ_RS22770"
                     /old_locus_tag="pgaptmp_004554"
     CDS             complement(95..661)
                     /locus_tag="ACHOQQ_RS22770"
                     /old_locus_tag="pgaptmp_004554"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4472708.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDKSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            complement(689..886)
                     /locus_tag="ACHOQQ_RS22775"
                     /old_locus_tag="pgaptmp_004555"
     CDS             complement(689..886)
                     /locus_tag="ACHOQQ_RS22775"
                     /old_locus_tag="pgaptmp_004555"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4472709.1"
                     /translation="MPQDDFCIVNPGGCIIHFPANGRYILDIHDPDFPEQYQRLLERL
                     DYLTKPGTKASGQRNSGYSGF"
     gene            complement(1134..1559)
                     /locus_tag="ACHOQQ_RS22780"
                     /old_locus_tag="pgaptmp_004556"
     CDS             complement(1134..1559)
                     /locus_tag="ACHOQQ_RS22780"
                     /old_locus_tag="pgaptmp_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4472710.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDSHALSAQQKATCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRATCTKSVYRYQPDLCQDCGGAST"
     gene            complement(1556..1939)
                     /locus_tag="ACHOQQ_RS22785"
                     /old_locus_tag="pgaptmp_004557"
     CDS             complement(1556..1939)
                     /locus_tag="ACHOQQ_RS22785"
                     /old_locus_tag="pgaptmp_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4472711.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWRCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            complement(2311..2904)
                     /locus_tag="ACHOQQ_RS22790"
                     /old_locus_tag="pgaptmp_004558"
     CDS             complement(2311..2904)
                     /locus_tag="ACHOQQ_RS22790"
                     /old_locus_tag="pgaptmp_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4472712.1"
                     /translation="MYNANPNYEMNFAILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRLDSTLSEICSLV"
BASE COUNT         1090 a          916 c          806 g          922 t
ORIGIN      
        1 acgccccaga ttaggggcgt cgaaatatgc ctctctgacc attatattcc ggcgtacagg
       61 tagcaggtca gaagtgacaa tgcgtcacct gacgttaaaa gtcactacac ccaagatgac
      121 gttcaacagc accatgcgat tcaatgtaag cccgggctgt ctgttccagt acaccaggct
      181 cagcgttgta tgtgttagct gcatcaaata ccaacgacag cacttcagga tacacaacca
      241 gatgtgtaat ggagttatct tcacccaata cttttcccca cgcctgctca atcagatttc
      301 tgagaaccac cacctcacga ctcttacacc agacatcgtt attaagtagc agcaccataa
      361 gataaggagt ggtatcgtta gccacagcct ccctactcca gagataatat aaaggggtgg
      421 gctcaacaga tttatcttta cgtcgcttac actgcaaata ttcagaaatg agtctatgca
      481 gttcaccagt aaaatcagcc atcaaagagg gaatggcctt attaataccc tgccctggta
      541 ttaatttaaa ttgtaataat ttaatttcag gatgtgtggc tgcagcccga tacagagttg
      601 caaggacaca cttttgccag agggcgttac aggaaagctt aacgtttgat tctgtataca
      661 taataaatca ccttacagtt acaacaggtc aaaaaccgct gtagccagag ttacgctggc
      721 ctgatgcttt agtaccgggc ttcgtcagat aatccagacg ctccaataag cgctgatact
      781 gctcagggaa atcaggatca tgaatatcca ggatgtaacg tccattagca gggaaatgaa
      841 taatgcagcc ccctggatta acaatgcaga aatcgtcctg aggcactgat caatacggag
      901 aggactctcg cgtgtggttt attgacacca cagtgcagat tcggcgaatc cgcgatcacg
      961 gtgcaatttc gttccacagc acacaatcat aaccccgggt tttattcagg taagcaggac
     1021 tgcggatatc cggtgtcgcg cctttgtgtc acgaacgggg taggtgcgaa acaccggata
     1081 aaatgcaggc tggcaatacc tctgaacgcc ctgcgcagag cggatatttt ggattaagta
     1141 ctcgcacctc cgcagtcctg gcacaaatct ggctggtacc tgtaaacaga cttcgtacat
     1201 gttgctctgc aataaatccc cgtaccacag gcctcacaga atttcttccc atgaaaaaac
     1261 tgctcacaca tcacacaatg ccactccagc acgaccggta atggcgatag aaacatcgcc
     1321 atatcctcaa tgtaagggtg ggacttttcc ggattcagca ccacgcaggt cgccttctgt
     1381 tgcgcgctca gggcatgtga atcatgctca aaccacgccc cctgagcatc tgtctgcaaa
     1441 atcaaccgac cacgacagga aaggcagaaa cactgccgga tatttctgct gagcgcagag
     1501 tcggcactga taatgtgttc acccggcgtg atccccagcc ccgtttttat accgttcatt
     1561 cagccactcc ctcctcactg aagtgccctg tatggcagtg tgtgcagtac cgctccccat
     1621 aataatcgtg gtgacattgt ctgcagcgcc agctggcttt acgcaccacg ggtaaggcat
     1681 ccgggacgaa ttgctgcaga cgtttaatca accgtatttc tctgcgttcc ggcctgacat
     1741 aaggacattg ctgcgcgtgc tccgtcagcc cttcgtctgc gtgttcaaac cagggacgct
     1801 cagtgtcgta ttgcgggtgg tatctgagcg cactgccgca aaggtggcaa gtgtagcgat
     1861 cgtaaggtgc agtctgtgcg gtacgggcac ccgtcagacg cccgttgcca tcaaatgcaa
     1921 taaaggattt tgcgtacatc gtcatattcc tcagccgcca gacgacacgc aggcaacagc
     1981 tgtcccggta cgggcagtgt ggtcagggtg tgaatggcgg ttcagttaaa atctggactg
     2041 gagtttgggt aaaacaggct ggttgctgtt accgcactgt ggttgtcaca caatggccca
     2101 cctgcgtaaa aaactgtcag caggcaggga tggacatcgg gggatgtgct gtatcggttg
     2161 ccggaaggaa tggtaaagaa tgtgccatgg ttaaatctcc gtatggttca tacggaatag
     2221 ccatgtcgta aaaaatgcgc agagtccctg aagtggcctc cggaaccgac tgtaacaatt
     2281 agcgccagcc cgtagttgtc gttcaggggc ttacaccaga gaacatatct ccgacagggt
     2341 actgtccagt cgcggtcggc cccggttact tttttcggga agcctgctac gccccagaat
     2401 aataccgtgt tctttctgat cctgtttagc caggtagctg agggcgtaac gtaagtcgtc
     2461 taccgcagat ttatcactat agtgaatcac gtgatcgata cgcaccgggt atttgtcttt
     2521 agcccggcac agatgaaaat aaccctcccc ttccgtgatc cgcctccaga tatctcccag
     2581 ttgcctggat atctgataag agttcttgtg acgttgtcca tccagatagc cgataaaatg
     2641 aatatgaagc cctttgtctg gtgtatattc cattacccag taatatcctg ctaacatcgt
     2701 ctgggtttcg ctaagtaaac ggtatatttc catacacata ctgtgcttac aggaatgccc
     2761 gaaactgggc gtgtctttcc tgtaggcaaa atcaattctg aaaggtaaca gtttagaaaa
     2821 acgttgaaac ataccgtcca tgtgttcatt cacatctttc agaatcgcga aattcatttc
     2881 gtaattggga ttggcattat acattttata gtccttactt tataaaactc acaggaagag
     2941 gaaattatgt aaatgctgag ggtacagaaa cgtcgtacta cggcattaat aaaagagata
     3001 accagcagca cagacaacct gataaaactg ttttgtatta cctcattaca gcaatgaggt
     3061 aatatttcag aatacagtga gactctctta aactgaggac cagtacaaat tacaatggta
     3121 aaaacaccac ctcatagaat tgagtttaag aaaacaccat tcactcaacc tgacattcac
     3181 aaaagtcagt accaggtagc ctcagataac atcgctatta atattatcct gccattaagt
     3241 aaaaacagaa actctgtgag gaagcctcac caatatttga ccagtagcat aaagaataac
     3301 gatatgagag atacgcccac accagagatg attagcccat acaaaaagat aatatttatt
     3361 aataccaccg aacctcaata gagtaataac acactaatag cgttttagta cacacttaac
     3421 actaactatg caccagacac atactcagga ttaacattct caacatttat ggtactctca
     3481 aagagtataa ttcaccacac aggtaaactc tcacaccagt agtatggaga taatgctgta
     3541 cacatactta cagagtaaag tgtacagcta gcatacagtg gcgagcagac attacaacat
     3601 aaagactcaa gatagcataa taaagcataa taagaacaca acaaaacccc cactaataat
     3661 actaattaac cttaattctt ttttgaacag gaaatcaaca tagctccaga cctcttcagg
     3721 tcatacactt ttgt
//
LOCUS       DAZKFU020000342         3712 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZKFU020000342 DAZKFU020000000
VERSION     DAZKFU020000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.344"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            187..786
                     /locus_tag="ACHOQQ_RS22795"
                     /old_locus_tag="pgaptmp_004559"
     CDS             187..786
                     /locus_tag="ACHOQQ_RS22795"
                     /old_locus_tag="pgaptmp_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4472713.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            983..1441
                     /gene="tnpA"
                     /locus_tag="ACHOQQ_RS22800"
                     /old_locus_tag="pgaptmp_004560"
     CDS             983..1441
                     /gene="tnpA"
                     /locus_tag="ACHOQQ_RS22800"
                     /old_locus_tag="pgaptmp_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HGU4472714.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            1562..>3712
                     /locus_tag="ACHOQQ_RS22805"
                     /old_locus_tag="pgaptmp_004561"
     CDS             1562..>3712
                     /locus_tag="ACHOQQ_RS22805"
                     /old_locus_tag="pgaptmp_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4472715.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSSASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAADSKTAAASSASAASTSAGQASASATAAGKSSESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSETLAATPKAVKTANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGA"
BASE COUNT         1012 a          929 c         1060 g          711 t
ORIGIN      
        1 ctgacactga cattcaccgt atcttcatca ggagcgaata actggacacc aacaaccagt
       61 atcagcgatc tgctggttgt ggtgatgaaa aaatccacag caggtatcag tatcagctga
      121 attttataac ccagaacggg cgtcagaaat gacgcctttt ttattgcaga aaagcgagag
      181 gtaattatgc gtaaacttta tgccgccatt ttgtccgcag ccatttgtct ggccgtatcc
      241 ggtgcgcctg catgggcatc tgaacatcag tccacgctga gcgcggggta tcttcatgcc
      301 cggacgaacg ttcccggcag tgatgatctg aacgggatta acgtgaaata ccgttatgag
      361 tttacggaca cgctgggaat ggtgacgtca ttcagctatg caggagacaa gaatcgccag
      421 cttacccgtt acagcgatac ccgctggcat gaagattccg tgcgtaaccg ctggttcagc
      481 gtgatggcgg ggccgtctgt acgcgtgaat gaatggttca gcgcgtatgc gatggcgggt
      541 gtggcttaca gccgtgtgtc gactttctcc ggggattatc tccgcgtaac tgacaacaag
      601 gggaaaacgc acgatgtgct gaccggaagt gatgacggtc gccacagcaa cacgtctctg
      661 gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga tgtcgcttat
      721 gaaggttccg gcagtggtga ctggcgcact gacggtttca tcgtgggtgt cggttataaa
      781 ttctgattag ccaggtaaca cagcgttatg acagcccgcc ggttcaggcg ggcttttttg
      841 tgcacagaaa acccccagct aggctggggg ttccggaaag ctttcagctt tgagccagtt
      901 attaaaaccc cttttgattt gttaaaacac cttgcggtct ggcaactgca agtgtcaaac
      961 aagaaatcaa aagggggtcc caatggggaa cgaaaagagc ttagcgcaca cccgatggaa
     1021 ctgtaaatat cacatagttt ttgcgccaaa ataccgaaga caggtgttct acagagagaa
     1081 acgtagagca ataggcagta ttttgagaaa gctgtgcgag tggaaaagcg tacggattct
     1141 tgaagctgaa tgctgtgcag atcatatcca tatgcttgtg gagatcccgc ccaaaatgag
     1201 cgtatccggc tttatgggat atctgaaagg gaaaagcagt ctgatgcttt acgagcagtt
     1261 tggtgatttg aaattcaaat acaggaacag agagttctgg tgcagagggt actacgtcga
     1321 tacggtgggt aagaacacgg cgaagataca ggattacata aagcaccagc ttgaagagga
     1381 taaaatggga gagcagttat cgattcccta tccgggcagc ccgtttacgg gccgtaagta
     1441 acgaagttgg atgcaaatgt cagatcgtgt gcgcctgtta gggcgcggct ggtaagagag
     1501 ccttataggc gcatttgaaa aacctccggc tatgccggag gatatttatt gtggggtgaa
     1561 tatggcagta aagatttcag gtgtactgaa agacggcaca ggaaaaccgg tacagaactg
     1621 cacaatccag ctgaaagcaa aacgtaacag caccacggtg gtggtgaaca cgctggcctc
     1681 agaaaatccg gatgaagccg ggcgttacag catggacgtt gagtacggtc agtacagcgt
     1741 tattctgttg gtggaagggt tcccgccgtc acatgccggg accatcaccg tgtatgaaga
     1801 ttctcaaccc ggtacgctga atgattttct tggtgccatg actgaggatg atgtccgtcc
     1861 ggaggcactg cgccgttttg agctgatggt ggaagaggtg gcgcgtaacg cgtccgcggt
     1921 ggcacagaac acggcagccg cgaagaagtc agccagcgat gccagcacat cagcccgtga
     1981 ggcggcaacc catgcgactg atgctgcgga ctcagcacgc gcagccagca cgtcagccgg
     2041 acaggccgcg tcgtcggctc agtcagcgtc ttccagcgca ggaacggcat caacaaaggc
     2101 tactgaagca tcaaaaagtg ctgccgctgc agagtcctca aaaagcgcgg cggctaccag
     2161 tgccggtgcg gcgaaaacgt cagaaacgaa tgcggcagcg tcacaacaat cagccgccac
     2221 ttctgcatcc accgcgacca cgaaagcgtc agaagctgcc tcctcagcca gggatgcgtc
     2281 ggcttcaaaa gaggcggcaa aatcatcaga aacgagtgca gcctcgagcg ccagtagttc
     2341 cgcttcctcg gcaacggcgg caggcaattc cgcgaaagcg gcaaaaacgt ccgagacgaa
     2401 cgctaagtct tctgaaacgg cagcggaaca gagtgcctcc gcagcagcag actcaaaaac
     2461 agcggctgca tcatctgcca gcgccgcgtc aacaagtgcc gggcaggcct cagccagtgc
     2521 caccgctgcc ggaaagtcgt cagaaagtgc cgcatcgtct gcttcaacag ccacaacgaa
     2581 ggctggcgaa gccactgaac aggccagcgc agcagcgagt tctgcttccg cagcgaagac
     2641 atccgaaacg aacgcgaaag cgtcggaaac cagcgcagaa tcctcaaaaa cggctgccgc
     2701 atcgtcagcc agttcggcgg cgtcatcggc atcatctgcg tctgcttcaa aagatgaggc
     2761 gaccagacaa gcgtcagcag cgaagagcag cgccacgacg gcatccacga aggcgacaga
     2821 ggcagctggt agtgcgacgg cagcagctca gagcaaaagt acggcggaat ctgcagcaac
     2881 gcgcgctgag acagcggcaa aacgggcaga ggatattgca tccgccgtgg cgcttgagga
     2941 tgcgagcacg acgaaaaagg ggatagtaca gctcagcagt gcgaccaaca gcacttccga
     3001 gtcactggcg gcaacgccaa aagcagttaa ggccgcgtat gacctggcta acgggaaata
     3061 cacagcacag gatgcaacga cagcacagaa agggatagtc caactaagca gcgcgaccaa
     3121 cagcacgtct gaaacgctgg cggcaacgcc aaaggcagta aaaacagcca atgacaatgc
     3181 tgagaaacgt ctgcagaaag atcagaacgg tgcggatatc cctggcaaag acacctttac
     3241 gaaaaatatt ggtgcctgcc gtgccttcgg tgggtcagta agcacaacaa caggaaattg
     3301 gacgactgca cagtttatcg agtggctgga ttctcaggga gcatttaacc atccatactg
     3361 gatgtgcaag ggttcctggt cttatggcaa taataaaatc attactgata ctggctgcgg
     3421 taatattcat ctcgccggag ctgtcattga agtaatgggg ataaagtcag cgatgacgat
     3481 ccgcattacc actccgacca catccagcgg tggcggaaca accaacgccc agtttaccta
     3541 tattaatcac ggaacagatt attcacctgg ctggcgaagg gactataact ccagaaataa
     3601 gccaacggca tcagagatcg gggcgctacc gtcaggtgga acagcagtat catcagttaa
     3661 tctggcttca aaaggtcggg tagccgcgct gacagacaat acgcaggggg ca
//
LOCUS       DAZKFU020000343         3685 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZKFU020000343 DAZKFU020000000
VERSION     DAZKFU020000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.345"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1301)
                     /locus_tag="ACHOQQ_RS22810"
                     /old_locus_tag="pgaptmp_004562"
     CDS             complement(<1..1301)
                     /locus_tag="ACHOQQ_RS22810"
                     /old_locus_tag="pgaptmp_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HGU4472716.1"
                     /translation="MKGRLITSDPWNQQFMVELEVFFSPRQPACSELISVFPNHSLQQ
                     IDGFGGSFTESAGVVFNSMSDKTKAQFLNLYFSPQEHNYSLGRMPIQSCDFSLSNYAY
                     VDSSEDLRQGRISFSRDESHLMPLISAALRINPQIKLMASPWSPPAFMKTNYDMNGGG
                     KLRYEYYADWADIIIDYLLEYRRHGISVKALSVQNEPVAIKSWDSCLYSVEEETTFAV
                     HYLRPRLARYGMNEIEIYIWDHDKDGLIDWAERAFADEKDGDSINGMAFHWYTGDHFS
                     QIQYLARKLPNKKLLFSEGCVPMENDEGGQIRHWHTYIHDMIGNFKSGCSGFIDWNLL
                     LDSDGGPNHQSNLCEAPVQYDALNDILWCNHSWYGIGHFCRFVHPGAKVMLTSSYDTQ
                     LEDVGFVNPDGERVLVVYNRDTRERHFRVVDGDRELALTLPP"
     gene            complement(1399..2409)
                     /gene="iolG1"
                     /locus_tag="ACHOQQ_RS22815"
                     /old_locus_tag="pgaptmp_004563"
     CDS             complement(1399..2409)
                     /gene="iolG1"
                     /locus_tag="ACHOQQ_RS22815"
                     /old_locus_tag="pgaptmp_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase IolG1"
                     /protein_id="HGU4472717.1"
                     /translation="MTLKAGIVGIGKIGADHLRRLANTISGVEVVAVCDIVEGRAQAA
                     LDKYEIKAKDYKDYHDLINDEDVEVVVITASNEVHADVAVAALNANKYVFCEKPLAVT
                     ADDCQRVIGAERKNGKRMVQVGFMRRYDKGYVQLKNIIDSGEIGQPLMVHGRHYNVST
                     VSDYKTPQAIYETLIHEIDVMHWLLNEDYRTVKVYFPRQSSLVTTLRDPQVVVMETTS
                     GINIVVEVFVNCQYGYDIHCDVTCEKGMVELPTVATAAVRKEAKYSTDILVDWKQRFI
                     DAYDIEFQDFFDRLNAGLPLAGPTSWDGYLAAVTADACVKSQETGNTEFVELPAKPDF
                     YK"
     gene            complement(2427..3332)
                     /gene="iolE"
                     /locus_tag="ACHOQQ_RS22820"
                     /old_locus_tag="pgaptmp_004564"
     CDS             complement(2427..3332)
                     /gene="iolE"
                     /locus_tag="ACHOQQ_RS22820"
                     /old_locus_tag="pgaptmp_004564"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HGU4472718.1"
                     /translation="MYSVKESIKWGIAPIGWRNDDLPEIGKDNTYKQILSDAALTGFS
                     GTEVGGCYPLDPTELNIELALRHLEIPGKWFSSFIIRDGIESVKKAFEQHCAYLQAVH
                     AYIAVVSEQTYSIQGIIDKCVYTEKPYFNDIEWQQLCSGLNELGMIANTYGLKLAFHH
                     HMGTGVQTLQEVDRLMENTDPQLVNLLFDTGHIYVSDGDVMSLLCKHFSRIKHVHFKD
                     VRDEKLKACRAGKKSFLNSFLDGMFTVPGDGNIDFKSVLAYLVENHYSGWIVVEAEQD
                     PNIYNPLEYAQKGKKHIDGLLGNYI"
BASE COUNT         1096 a          880 c          656 g         1053 t
ORIGIN      
        1 ggcggcagcg ttaatgcgag ttctctgtcg ccatcgacca cccggaaatg cctttctcgc
       61 gtatcgcgat tatataccac taaaactcgc tcaccatcag gattaacaaa acccacatct
      121 tccagttgcg tatcataact ggaagtaagc ataactttcg caccgggatg cacaaagcgg
      181 caaaagtgtc caattccgta ccaagagtgg ttacaccaga gaatgtcatt caatgcatcg
      241 tactgcacag gagcttcgca caaattactc tggtgatttg ggcctccatc actgtctaac
      301 agcaggttcc agtcgataaa tccgctacag ccggatttaa aattaccaat catgtcatga
      361 atataggtat gccagtgacg gatctggcca ccttcatcat tctccattgg tacacatcct
      421 tcggaaaaca gcagcttttt attcggcaac ttccgggcca gatactgaat ttgcgaaaaa
      481 tggtcaccgg tataccagtg gaaagccatc ccgttgatgc tatcgccatc cttttcgtca
      541 gcaaaggcac gttcagccca gtcaatcaga ccatctttat catgatccca gatatagatc
      601 tctatttcat tcattccata gcgggcgagt cgtgggcgca gatagtgaac agcaaaagta
      661 gtctcttctt ctacactata cagacaggaa tcccagcttt ttatcgcaac tggttcattc
      721 tgcactgata gtgctttaac actgatacca tggcgtctgt attcgagcag atagtcgata
      781 ataatatctg cccagtcagc atagtattca taccgcaatt tcccacctcc gttcatatca
      841 taattagtct tcatgaatgc cggtggactc cacggggaag ccattaattt tatttgtgga
      901 ttgatccgca atgccgcgga aattagcggc attaaatgag attcatcacg ggaaaaggaa
      961 atccttccct gacgcaggtc ttcactggaa tcaacatagg cataattgct cagagagaaa
     1021 tcacagcttt ggatcggcat ccgccccaaa ctatagttat gttcctgagg cgaaaaatag
     1081 agattcaaaa attgcgcctt tgtcttatca ctcatgctgt tgaaaacaac accagcactt
     1141 tcagtgaagc tgccgccaaa tccatcaatc tgttgtaacg agtggtttgg aaagacgctg
     1201 attaattcac tacacgctgg ttgacgaggc gaaaaaaaaa cctccagctc aaccataaat
     1261 tgctgattcc agggatcgga agtgattagt ctgcctttca tagagggatt ccagataata
     1321 taggatgata aattgtgata taccggacag gagcatacac ctagctatcc ggtatgaaat
     1381 tatcacatcg cagaaagact atttgtagaa gtcaggtttt gctggtaatt ccacaaactc
     1441 ggtgtttcct gtttcctggg acttcacaca ggcatcagcc gttaccgcag ccagataacc
     1501 atcccatgat gttgggcctg ccagaggtaa tcccgcattc agacggtcaa agaagtcctg
     1561 gaactcaata tcataggcat caataaagcg ttgtttccag tccaccaaaa tgtcagtact
     1621 gtattttgcc tctttacgca ccgcagcagt agccacggta ggtaattcca ccatcccttt
     1681 ctcacaggtc acatcacaat gaatatcata gccatactgg caattcacaa acacctcaac
     1741 cactatatta ataccggagg tcgtttccat caccaccacc tgcggatctc gtagagtagt
     1801 caccaagctg gactgacgtg ggaagtagac cttaactgtt ctataatctt cgttgagtaa
     1861 ccagtgcata acatcgattt catgaatcag cgtttcataa attgcttgtg gtgttttgta
     1921 atccgatacc gtgcttacat tataatgacg accatgaacc attaatggtt gaccaatttc
     1981 tccgctatcg atgatgtttt tcagctgtac gtagccttta tcgtagcgac gcataaaccc
     2041 aacttgcacc attcgttttc catttttacg ttcagctccg atcacacgct gacaatcatc
     2101 tgctgtaacc gcaagcggct tttcacagaa aacatattta ttggcattta acgctgcaac
     2161 ggctacatcg gcatgtactt cattcgaggc agtaattacc actacttcga catcttcatc
     2221 attaatcaga tcgtgatagt ctttatagtc ttttgctttg atctcatact tatccaaagc
     2281 tgcttgcgct ctgccttcga caatatcgca cacagcaaca acttctactc cagaaatagt
     2341 gttagccaga cgtcttaagt gatcggcacc aattttcccg atccccacaa tacctgcttt
     2401 taaagtcatc ttattttcct tttctatcag atataattac ccaataaacc atcaatatgc
     2461 tttttacctt tttgtgcata ttccaaagga ttatatatat tcgggtcctg ctcagcttca
     2521 acgacaatcc agccggaata atgattctca accaaataag ccaatacaga tttaaaatca
     2581 atattcccat caccaggaac agtaaacatt ccatcaagaa aagaattgag aaatgatttt
     2641 tttccagccc gacatgcttt tagtttttca tcccgaacat ctttaaagtg aacatgttta
     2701 atacgggaaa aatgcttgca caacagtgac atcacatctc catcagaaac ataaatatga
     2761 ccagtatcaa acagcaaatt cactaattgt ggatctgtgt tttccataag tcgatccact
     2821 tcctgtaaag tttgtacgcc agtgcccata tgatggtgga aagcaagttt aagcccatat
     2881 gtattggcga tcatgcctaa ctcgttcaat cctgagcata attgttgcca ttcgatatca
     2941 ttgaagtaag gtttttccgt atagacacat ttatcaataa ttccttgaat actatacgtt
     3001 tgctcagaca caacagcgat ataagcatgg acagcctgca aataagcgca atgttgttca
     3061 aaagctttct tcactgattc aataccatca cgaataataa aagatgagaa ccactttccg
     3121 ggaatttcca gatggcgaag tgctaactct atatttaatt cagtcgggtc cagaggataa
     3181 catcccccca cttctgttcc agaaaaacca gtaagtgctg catcacttaa tatctgctta
     3241 taggtattgt ctttaccaat ttcaggcaaa tcatcatttc gccatccgat tggcgcaatc
     3301 ccccatttaa tactctcttt tacactatac atattccaga ctcctgctta ataaaacttt
     3361 ctcacatcct gttgcataag taataatctt tggctgacaa tatgctactt tccacgtggc
     3421 taaaaaactt tatctatcat gagtacagaa gtgggaaaaa tgtgtcagga atcacaaaaa
     3481 aaacacaacc ctttaatgat tttgtctatg ttttagtcat gctgaaaaat cacacaatga
     3541 ccgtaaaata aaaccgtcac tttcccacta actctggcgt ggacagaatc atcccgtatg
     3601 attcccaagt ttggtaaata ttattaaatc aggatgtgca agctatgatt cagtcatact
     3661 aggaaaaaag tttttttgca gattc
//
LOCUS       DAZKFU020000344         3663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZKFU020000344 DAZKFU020000000
VERSION     DAZKFU020000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.346"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..252
                     /locus_tag="ACHOQQ_RS22825"
                     /old_locus_tag="pgaptmp_004565"
     CDS             <1..252
                     /locus_tag="ACHOQQ_RS22825"
                     /old_locus_tag="pgaptmp_004565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472719.1"
                     /translation="CKWWDLADLLSFLSFVMEDFIVSFHLSSCLFLLPGVYVSGCQLL
                     KQDRTVNVFYTFFPSNSEAGHGDVVWSRDGRPIAYRSGR"
     gene            194..1918
                     /gene="ilvI"
                     /locus_tag="ACHOQQ_RS22830"
                     /old_locus_tag="pgaptmp_004566"
     CDS             194..1918
                     /gene="ilvI"
                     /locus_tag="ACHOQQ_RS22830"
                     /old_locus_tag="pgaptmp_004566"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGU4472720.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRSNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1921..2412
                     /gene="ilvN"
                     /locus_tag="ACHOQQ_RS22835"
                     /old_locus_tag="pgaptmp_004567"
     CDS             1921..2412
                     /gene="ilvN"
                     /locus_tag="ACHOQQ_RS22835"
                     /old_locus_tag="pgaptmp_004567"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4472721.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2424..2510
                     /locus_tag="ACHOQQ_RS22840"
                     /old_locus_tag="pgaptmp_004568"
     CDS             2424..2510
                     /locus_tag="ACHOQQ_RS22840"
                     /old_locus_tag="pgaptmp_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGU4472722.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2592..3596
                     /gene="cra"
                     /locus_tag="ACHOQQ_RS22845"
                     /old_locus_tag="pgaptmp_004569"
     CDS             2592..3596
                     /gene="cra"
                     /locus_tag="ACHOQQ_RS22845"
                     /old_locus_tag="pgaptmp_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGU4472723.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          850 a          871 c         1023 g          919 t
ORIGIN      
        1 tgtaagtggt gggatttagc cgatttatta tcatttttat ccttcgtaat ggaggatttt
       61 atcgtttctt ttcacctttc ctcctgttta ttcttattac ccggcgttta tgtctctggc
      121 tgccaattgc ttaagcaaga tcggacggtt aatgtgtttt acacattttt tccgtcaaac
      181 agtgaggcag gccatggaga tgttgtctgg agccgagatg gtcgtccgat cgcttatcga
      241 tcagggcgtt aaacaagtat tcggttatcc cggaggcgca gtccttgata tttacgatgc
      301 attgcatacc gtgggtggta tcgatcatgt tttagttcgt catgagcagg cggcagtgca
      361 tatggccgat ggtctggcgc gcgcgaccgg ggaagtcggc gtcgtgctgg taacgtcggg
      421 tccaggggcg accaatgcga ttactggcat cgccaccgct tatatggatt ccattccatt
      481 agttgtcctt tccgggcagg tagcgacctc gttgataggt tacgatgcct ttcaggagtg
      541 cgacatggtg gggatttcgc gaccggtggt taaacacagt tttctggtta agcaaacgga
      601 agacattccg caggtgctga aaaaggcttt ctggctggcg gcaagtggtc gcccaggacc
      661 agtggtcgtt gatttaccga aagatattct taatccggcg aacaaattac cctatgtctg
      721 gccggagtcg gtcagtatgc gttcttacaa tcccactact accggacata aagggcaaat
      781 taagcgtgct ctgcaaacgc tggtagcggc aaaaaaaccg gttgtctacg taggcggtgg
      841 ggcaatcacg gcgggctgcc atcagcagtt gaaagaaacg gtggaggcgt tgaatctgcc
      901 cgttgtttcc tcattgatgg ggctgggggc gtttccggca acgcatcgtc aggcactggg
      961 catgctggga atgcacggta cctacgaagc caatatgacg atgcataacg cggatgtgat
     1021 tttcgccgtc ggggtacgat ttgatgaccg aacgacgaac aatctggcaa agtactgccc
     1081 aaatgccact gttctgcata tcgatattga tcctacttcc atttctaaaa ccgtgactgc
     1141 ggatatcccg attgtggggg atgctcgcca ggtcctcgaa caaatgcttg aactcttgtc
     1201 gcaagaatcc gcccatcaac cactggatga gatccgcgac tggtggcagc aaattgaaca
     1261 gtggcgcgct cgtcagtgcc tgaaatatga cactcacagt gaaaagatta aaccgcaggc
     1321 ggtgatcgag actctttggc ggttgacgaa gggagacgct tacgtgacgt ccgatgtcgg
     1381 gcagcaccag atgtttgctg cactttatta tccattcgac aaaccgcgtc gctggatcaa
     1441 ttccggtggc ctcggcacga tgggttttgg tttacctgcg gcactgggcg tcaaaatggc
     1501 gttgccagaa gaaaccgtgg tttgcgtcac tggcgacggc agtattcaga tgaacattca
     1561 ggaactgtct accgcgttgc aatacgagtt gcccgtactg gtggtgaatc tcaataaccg
     1621 ctatctgggg atggtgaagc agtggcagga catgatctat tccggccgtc attcacaatc
     1681 ttatatgcaa tcgctacccg atttcgtccg tctggcggaa gcctatgggc atgtcgggat
     1741 ccagatttct catccgcatg agctggaaag caaacttagc gaggcgctgg aacaggtgcg
     1801 cagtaatcgc ctggtgtttg ttgatgttac cgtcgatggc agcgagcacg tctacccgat
     1861 gcagattcgc gggggcggaa tggatgaaat gtggttaagc aaaacggaga gaacctgatt
     1921 atgcgccgga tattatcagt cttactcgaa aatgaatcag gcgcgttatc ccgcgtgatt
     1981 ggcctttttt cccagcgtgg ctacaacatt gaaagcctga ccgttgcgcc aaccgacgat
     2041 ccgacattat cgcgtatgac catccagacc gtgggcgatg aaaaagtact tgagcagatc
     2101 gaaaagcaat tacacaagct ggtcgatgtc ttgcgcgtga gtgagttggg gcagggcgcg
     2161 catgttgaga gggaaatcat gctggtgaaa attcaggcca gcggttacgg gcgtgacgaa
     2221 gtgaaacgta atacggaaat attccgtggg caaattatcg atgtcacacc ctcgctttat
     2281 accgttcaat tagcaggcac cagcgataag cttgatgcat ttttagcatc gattcgcgat
     2341 gtggcgaaaa ttgtggaagt tgctcgctct ggtgtggtcg gactttcgcg cggcgataaa
     2401 ataatgcgtt gagaatgatc tcaatgcgca atttacagcc caacatgtca cgttgggctt
     2461 tttttgcgaa atcagtggga acctggaata aaagcagttg ccgcagttaa ttttctgcgc
     2521 ttagatgtta atgaatttaa cccataccag tacaatggct atggttttta cattttacgc
     2581 aaggggcaat tgtgaaactg gatgaaatcg ctcggctggc gggagtgtcg cggaccactg
     2641 caagctatgt tattaacggc aaagcgaagc aataccgtgt gagcgacaaa accgttgaaa
     2701 aagtcatggc tgtcgtgcgt gagcacaatt accacccgaa cgccgtggca gctgggcttc
     2761 gtgctggacg cacacgttct attggtcttg tgatccccga tctggagaac accagctata
     2821 cccgcatcgc taactatctt gaacgccagg cgcggcaacg ggggtatcaa ctgctgattg
     2881 cctgctcaga agatcagcca gacaacgaaa tgcgctgtat tgagcacctt ttacagcgtc
     2941 aggtcgatgc cattattgtt tcgacgtcgt tgcctcctga gcatcctttt tatcaacgct
     3001 gggctaacga cccgttcccg attgtcgcgc tggaccgcgc cctcgatcgt gaacacttta
     3061 ccagcgtggt tggtgccgat caggatgatg ccgaaatgct ggcggaagag ttacgtaagt
     3121 ttcccgccga gacggtgctt tatcttggcg cgctgccgga gctttctgtc agcttcctgc
     3181 gtgaacaagg tttccgtact gcctggaaag atgatccgcg cgaagtgcat tttctgtatg
     3241 ccaacagcta tgagcgggag gcggctgccc agttattcga aaaatggctg gaaacgcatc
     3301 cgatgccgca ggcgctgttc acaacgtcgt ttgcgttgtt gcaaggggtg atggatgtca
     3361 cgctacgtcg cgatggtaaa ctgccttctg acctggcaat tgccaccttt ggcgataacg
     3421 aactgctcga cttcttacag tgcccggtgc tggcagtggc tcaacgtcac cgcgatgtcg
     3481 cagagcgtgt gctggagatt gtcctggcaa gcctggacga accgcgtaag ccaaaacctg
     3541 gtttaacgcg cattaaacgt aatctctatc gccgcggtgt gctcagccgt agctaagctg
     3601 cgaacaaaaa tacgcgccag gtgaatttca ctctggcgcg tagagtacgg gactggacat
     3661 caa
//
LOCUS       DAZKFU020000345         3662 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZKFU020000345 DAZKFU020000000
VERSION     DAZKFU020000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.347"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            160..1263
                     /gene="proB"
                     /locus_tag="ACHOQQ_RS22850"
                     /old_locus_tag="pgaptmp_004570"
     CDS             160..1263
                     /gene="proB"
                     /locus_tag="ACHOQQ_RS22850"
                     /old_locus_tag="pgaptmp_004570"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGU4472724.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1275..2528
                     /gene="proA"
                     /locus_tag="ACHOQQ_RS22855"
                     /old_locus_tag="pgaptmp_004571"
     CDS             1275..2528
                     /gene="proA"
                     /locus_tag="ACHOQQ_RS22855"
                     /old_locus_tag="pgaptmp_004571"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGU4472725.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLDKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRV"
     gene            2643..2718
                     /locus_tag="ACHOQQ_RS22860"
                     /old_locus_tag="pgaptmp_004572"
     tRNA            2643..2718
                     /locus_tag="ACHOQQ_RS22860"
                     /old_locus_tag="pgaptmp_004572"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2676..2678,aa:Thr,seq:cgt)
     gene            complement(2845..3252)
                     /locus_tag="ACHOQQ_RS22865"
                     /old_locus_tag="pgaptmp_004573"
     CDS             complement(2845..3252)
                     /locus_tag="ACHOQQ_RS22865"
                     /old_locus_tag="pgaptmp_004573"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4472726.1"
                     /translation="MVVASPSYLAANSTPVHPRELVNHKCIGWRQSPDVAPYRWPFEE
                     NRRAFDLAIEPQITTNDLRLMLRLALAGGGITIATQETFRPYIESGRLVSLLDDFLPQ
                     FPGFYLYFPQRRNIAPKLRALIDHVKEWRQQLA"
     gene            3258..3524
                     /locus_tag="ACHOQQ_RS22870"
                     /old_locus_tag="pgaptmp_004574"
                     /pseudo
     CDS             3258..3524
                     /locus_tag="ACHOQQ_RS22870"
                     /old_locus_tag="pgaptmp_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT          914 a          911 c          991 g          846 t
ORIGIN      
        1 tagtaaatct gtaataaaag cgtaaacaac tgccgctacg cttgctgatc ccgcgcaaca
       61 aaacaccatg ctttgctcgc agatggttgg caaccgacga cagtcctgct aaaacgttcg
      121 tttgatatca tttttcctaa aattgaatgg cagagaatca tgagtgacag ccagacgctg
      181 gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa ccgtgcccat
      241 atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg gattgttatt
      301 gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg gttacccgga actgccagcg
      361 actatcgcct cgaaacaact gctggcggcg gtagggcaga gtcgactgat tcaactgtgg
      421 gaacagctgt tttcgattta tggcattcac gtcgggcaaa tgctgctgac tcgtgctgat
      481 atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt gctcgataac
      541 aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta cggcagagat taaagtcggc
      601 gataacgaca acctttctgc actggcggcg attctggcgg gtgccgataa actgttgtta
      661 ctgaccgatc aaaaaggttt gtataccgct gacccgcgca gtaatccgca ggcagaattg
      721 ataaaagatg tttacggtat tgatgacgca ctgcgcgcga ttgccggtga cagcgtttca
      781 ggcctcggaa ctggcggcat gagtaccaaa ttgcaggccg cggacgtggc ttgccgtgcg
      841 ggtatcgaca ccattattgc cgcgggcagc aagccgggcg tcattggtga tgtgatggaa
      901 ggcatttccg tcggtacgct gttccatgcc caggcgactc cgcttgaaaa ccgtaaacgc
      961 tggattttcg gtgcgccgcc tgcgggtgaa atcacggtag atgaaggggc aaccgccgcc
     1021 attcttgaac gcggcagctc cctgttgccg aaaggcatta aaagcgtgac tggcaacttc
     1081 tcgcgtggtg aagtcatccg catttgtaac ctcgaaggtc gcgatatcgc ccacggcgtc
     1141 agtcgttaca acagcgatgc attacgccgt attgccggac accactcgca agaaattgat
     1201 gcaatactgg gatatgaata cggcccggtt gccgttcacc gtgatgacat gattacccgt
     1261 taaggagcag gctgatgctg gaacaaatgg gcattgccgc gaagcaagcc tcgtataaat
     1321 tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga taaaatcgcc gatgaactgg
     1381 aagcacaaag cgaaatcatc ctcaacgcta acgcccagga tgttgctgac gcgcgtgcca
     1441 atggcctcag cgaagcgatg cttgaccgtc tggcactgac gcccgcacgg ctgaaaggca
     1501 ttgccgatga tgtgcgccag gtgtgcaacc tcgccgatcc ggtggggcag gtaatcgatg
     1561 gcggcgtact ggacagcggc ctgcgtcttg agcgtcgtcg cgtaccgctg ggggttattg
     1621 gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt cgcttcgctg tgcctgaaaa
     1681 caggtaacgc ggtgatcctg cgcggtggca aagaaacctg tcgcactaac gctgcaacgg
     1741 tggcggtgat tcaggacgcc ctgaaatcct gtagcttacc ggctggtgcc gtgcaggcga
     1801 ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg tatggataaa tacatcgaca
     1861 tgctgatccc gcgcggcggg gctggtttgc ataaactgtg ccgcgagcag tcgacgatcc
     1921 cggtgatcac aggtggtata ggcgtatgcc atatttacgt tgatgaaagt gcagagatcg
     1981 ctgaagcatt aaaagtgatc gtcaacgcga aaactcagcg tccgagcaca tgtaatacgg
     2041 tagaaacgtt gctggtgaat aaaaacatcg ccgatagctt cctgcccgca ttaagcaaac
     2101 aaatggcgga aagcggcgtg acattacacg cagatgcagc tgcgctgaca cagttgcagg
     2161 caggccccgc gaaggtggtg gcggttaaag ccgaagagta tgacgatgag tttctgtcat
     2221 tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc catcgcccat attcgtgaac
     2281 acggcacaca acactccgat gcgatcctga cccgcgatat gcgcaacgcc cagcgttttg
     2341 ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc tacgcgtttt accgacggcg
     2401 gccagtttgg actgggtgcg gaagtggcgg taagcacaca aaaactccac gcgcgtggcc
     2461 caatggggct ggaagcactg accacttaca agtggatcgg cattggtgat tacaccattc
     2521 gtgtgtaaat aaaaccgggt gatgcaaaag tagccatttg attcacaagg ccattgacgc
     2581 atcgcccggt tagttttaac cttgtccacc gtgattcacg ttcgtgaaca tgtcctttca
     2641 gggccgatat agctcagttg gtagagcagc gcattcgtaa tgcgaaggtc gtaggttcga
     2701 ctcctattat cggcaccatt taaatcaagt agttacccca tattcaaata caccacgttt
     2761 cctcctgtgc cgtatttatg ccattgtaac cctggcaatt aatcaaaata ctgttctgac
     2821 atcaggcaat gcaggtgcag acatttaagc caattgctgc cgccattcct tgacgtggtc
     2881 aatcagggcg cggagctttg gtgcaatatt gcgacgctgt gggaaataca gatagaagcc
     2941 cggaaattgt ggaagaaagt catcaagcag agatacaagc ctaccgcttt caatatatgg
     3001 cctgaaagtt tcctgagtgg caattgttat tcctccgccg gcaagagcca gcctcaacat
     3061 cagacgcaga tcattagtcg taatctgcgg ttcaatcgca aggtcgaaag ctctcctgtt
     3121 ttcttcaaat ggccagcgat aaggcgcaac atccggggat tgacgccagc cgatacactt
     3181 atgattcaca agttcacgcg gatgaacagg tgtgctgttg gctgcgagat aggagggaga
     3241 ggcaacgacc atttcacgct gcctgatttg cagtacgggc tatttctgat ggcttacggc
     3301 aggaaagctc gaaaatccgt gtgacctgcg taaatcctgg cgttgtcgaa agtgaactgg
     3361 cttcaaccat tacccatgtg gaaaccatga aggcaatgga cgcctaccgt tctgttgcac
     3421 ttcaacctgc ggatattgcc cgtgcggtaa ggcatattat tgaatctccc gaaagcgttg
     3481 atacaaccga aattaccatc agacccactg catctgcaaa ctgaagaata acttttacat
     3541 tcatgtttta ttaaataggt cagaaggaag atttatatat ccctgtgttt ttcatagcaa
     3601 gatgcattat gtattgctca ccccgaagga gatgaaggtt taattacaga aggagtattt
     3661 tc
//
LOCUS       DAZKFU020000346         3655 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZKFU020000346 DAZKFU020000000
VERSION     DAZKFU020000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.348"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(50..769)
                     /gene="dcuR"
                     /locus_tag="ACHOQQ_RS22875"
                     /old_locus_tag="pgaptmp_004575"
     CDS             complement(50..769)
                     /gene="dcuR"
                     /locus_tag="ACHOQQ_RS22875"
                     /old_locus_tag="pgaptmp_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGU4472727.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(766..2397)
                     /locus_tag="ACHOQQ_RS22880"
                     /old_locus_tag="pgaptmp_004576"
     CDS             complement(766..2397)
                     /locus_tag="ACHOQQ_RS22880"
                     /old_locus_tag="pgaptmp_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGU4472728.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAMARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2578..2808
                     /gene="yjdI"
                     /locus_tag="ACHOQQ_RS22885"
                     /old_locus_tag="pgaptmp_004577"
     CDS             2578..2808
                     /gene="yjdI"
                     /locus_tag="ACHOQQ_RS22885"
                     /old_locus_tag="pgaptmp_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGU4472729.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKCRHK"
     gene            2820..3092
                     /locus_tag="ACHOQQ_RS22890"
                     /old_locus_tag="pgaptmp_004578"
     CDS             2820..3092
                     /locus_tag="ACHOQQ_RS22890"
                     /old_locus_tag="pgaptmp_004578"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4472730.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3319..3615
                     /gene="ghoS"
                     /locus_tag="ACHOQQ_RS22895"
                     /old_locus_tag="pgaptmp_004579"
     CDS             3319..3615
                     /gene="ghoS"
                     /locus_tag="ACHOQQ_RS22895"
                     /old_locus_tag="pgaptmp_004579"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4472731.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
BASE COUNT          955 a          826 c          893 g          981 t
ORIGIN      
        1 tattttgcat tacggtaata attattactt acagataacc agcgatccgt tattggcaat
       61 attgtttcag tagtgagtag tgttctgcct gaatacggta acggtaaact ggacgccccg
      121 tgacgccata atggatactg gtgaacaaga tgtggcagtt gaccagccag atgaggtatt
      181 tacggcagga aacacgcgag atgttaacct cgttagccag ctcgtcggtt gaaaactcat
      241 agtcctgatg cgcgtcaatc cactggcaca gcgtgcgtaa cgtctgcggc gttaagcctt
      301 ttggcaagcg acgaggatcc tgttcgttgg agctgctgcc gtggattagc tgatcaagct
      361 cggcctggtc ataatactga tgtttttcca gcgccatttt cttttgccgc cagccggtga
      421 gcgcctcttc aaagcgggaa gcctggaagg gtttgatcag gtaatccacg acaccgtaat
      481 gcagcgaatc tttaatggtt gccgcatcgg ctgcggagga gatgacaatc acatcacttt
      541 tgcaacgcgc gttatgcagg acaggcagta aatcgagccc gttctctttt tgcatataga
      601 tatcgagcaa tatcaggtcg ataggcgtat cgctattgaa gataatctct ttggctttct
      661 ccagcgtcga ggctgttcca cagcattgaa agcctgggat ttgtgctacg tatcggcgat
      721 tcagctccgc gaccattgcg tcgtcatcga taattaatac attgatcatc tgttcgacct
      781 ctccccgtcc cagggtatct ggacaaaaaa ttgtgtgaaa atcccgggtt ccgattccac
      841 ggcgatgctg ccgccgagat tttctacctg ttgtttgaca agtgctaaac cgacgcctcg
      901 ctcgcttcct tttgtcgaga cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat
      961 ccccggtcca tcatcgttaa cttcgcagtg cagccagccg tgacggtagt gcaatgttac
     1021 gctaatttcg ccaccgggtt ccggccctaa tgcttccagc gcgttttcta tcagatttcc
     1081 caatgtggta atcagcgtcg cgacttggtc ctcactgccg ctgtctggca gcaggctttc
     1141 actgtttaaa atcagcgtat ggcctaaatc ggtcgcgcgg ttaatcttgc tgattaaaaa
     1201 accagcgata accggagatt tgatcttacc cagcagagag ccaatctctt cctgatagtt
     1261 attggctgtt ttgagaatgt aatcttccaa ctgcttataa ctcttcagat gcaataatcc
     1321 gagaatcaca tgcaatttat tcataaattc gtgggatcgt tcacgaagtg catcagcata
     1381 gttgaccaga ccgtcgagtc gctgcatcag tttacgaact tcagttttgt ccctgaaggt
     1441 tgaaatggca ccgatgataa cgccattact gcgcaccgga acggtgttga tcagtaatag
     1501 ccggtcttta atcgtaatct cttcgtcgcg gcgcggcgta ccgtcgcgta acacttccga
     1561 gacatctacc acctgtgacc atgagtggct tagcgtcgac agtttctcat cgtcctgcga
     1621 cttacggtaa ttcagcaatt cttgtgcggc atcgttgatc agcgtgacct cgccgcgatc
     1681 gtccacggca acgacgcctt ctttgataga ctgcaacatg gcctggcgct gctcaaacag
     1741 cgtggagatt tcgtagggtt ccaggccgaa aaggattttt ttcagtacct taaccagaat
     1801 gcaggtgcca atcagtccga ccagcatgcc aaataatacc gaccagataa tgctccagcg
     1861 actgtcattg atttgttggg tcacgcggct taactcaagg ccgatcgcca ccacgccaat
     1921 ttgtttatga ttttcatcgt agatgggggt aaatacgcgt aaagcctgtg ccagaaaacc
     1981 gcgattgata gcgacatttt ctttgccatt cagcgcttta aggatgtcat cacctttaaa
     2041 tggctgacca atacgctggg cttcaggatg cgagtagcga agactttgca tatcggtaac
     2101 gacaataaac agcagatcgt tgcgtttgcg cacggcttcc gcgatggcct ggattccgct
     2161 ctcctgcggt tttttctgca agccctgacg gatttccggc gagtcggcga gggtacgcgc
     2221 cattgccagt gccttgttgg ccagcccgtc tcgcgtcata tcactgattt gcgagaagta
     2281 aatcagatgc accaccaata gcaccgagaa cagtaccgcg ctgaccatta agatcactgt
     2341 ggtactcaat ttcatcggac gtttgcgtaa catgcggtag ggcaatgaat gtctcatcag
     2401 cttccttgtg tgacaaattt cttaagcatt atctctgatg aggcgggtaa ttcaaaggga
     2461 gtaagaatga ttggctatat aggggaagag actctggcaa cggaaactgc cagtgctgta
     2521 tgaagattcc agggctatgc ttatagcgat aatcatactg atgagagagg gaaagtcatg
     2581 gatcaggcgc tactggacga gggttatcgc tgttataccg gcgaaaagat cgatgtctat
     2641 ttcaacactg cgatatgtca gcattctggc aattgcgtac gtggcaacgg caagttattt
     2701 aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg acgtcgtcac tgtggttaaa
     2761 gtgattgata cgtgcccgag cggcgcgctg aaatgccgtc ataaataagc gagggtaaaa
     2821 tggaaatacg cgaaggccac aataaatttt acattaatga cgaacagggc aagcaaatcg
     2881 ctgaaattgt ctttgtgccg accggagaga atttagcgat tatcgaacat accgatgtcg
     2941 atgaaagcct gaaagggcag gggattggta aacagctggt tgcgaaagtc gtggaaaaaa
     3001 tgcgtcggga aaaacgaaaa attatcccac tatgcccatt tgcgaaacac gaatttgata
     3061 aaacgcggga gtatgatgat attcgcagtt gatgggagag tacagagtca cgatattttt
     3121 catgctctcc gcggagtgat gcaggagagc atctgaaggg tagggggatg cacaaagaat
     3181 gggcagagag cgcgtttttt tgtcccaagt catccccttt actgagcaaa aaaaagaata
     3241 tctcctatat gagaatcatc aatcggggtt aataagtttt gcgtccccag agcattcaat
     3301 attgatagga gtcatattat ggaaggtaaa aacaagttca atacttatgt tgtttctttt
     3361 gattatccat catcttattc ctcagtgttc ttaaggttaa gatcattgat gtatgatatg
     3421 aatttctcct ctatcgtggc tgatgaatat gggataccac gacaattgaa tgaaaactcc
     3481 ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag atttaatcag gcttaaatgc
     3541 ttagacttac cggatattga ttttgacctc aacattatga cagttgatga ctatttccgt
     3601 cagttttaca agtagccagg aaggataaga agaacataaa aaatggcact attct
//
LOCUS       DAZKFU020000347         3616 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZKFU020000347 DAZKFU020000000
VERSION     DAZKFU020000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.349"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..1529
                     /locus_tag="ACHOQQ_RS22900"
                     /old_locus_tag="pgaptmp_004580"
     CDS             3..1529
                     /locus_tag="ACHOQQ_RS22900"
                     /old_locus_tag="pgaptmp_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4472732.1"
                     /translation="MSKKFTKTLLSSSLGGLLLGISSGVLAQELPPIKDSGLPFYSVL
                     KEADASSVIHGSTYRIYYNKSSGWGTMRYVLNGKDETLFNFDQEGNIIVLSKDNSIAY
                     TVHEPVLKDFARMAAGLRTSDKENGKHQVDEEEVRRIFNKVNNLSNTIINPEVLKQSD
                     FIASGPRPEGDPLVQASAVGEFALAVGEGAAAEEKNSTAVGSWASAHGRQSTALGSGG
                     QAYANGSTALGSYSHVDDSSVYGVALGGSSVVGENAPEGTALGSRAEVSHRNSVALGA
                     RAHTTRDNEVYIGYVEEAGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQD
                     TNTRLVVEAKKSREYTDSRTTVGVNSDGTLTSADGATKTIAVNDGLVALSGRTDRIDA
                     AVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQIRENHEEMKRAAAQSAALAGLF
                     QPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSYNVGVNFEF
                     "
     gene            1616..2089
                     /locus_tag="ACHOQQ_RS22905"
                     /old_locus_tag="pgaptmp_004581"
     CDS             1616..2089
                     /locus_tag="ACHOQQ_RS22905"
                     /old_locus_tag="pgaptmp_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472733.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            2460..>3616
                     /locus_tag="ACHOQQ_RS22910"
                     /old_locus_tag="pgaptmp_004582"
     CDS             2460..>3616
                     /locus_tag="ACHOQQ_RS22910"
                     /old_locus_tag="pgaptmp_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HGU4472734.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDNSGTVVPAVAGGDIVWKYSQGSGE
                     GTLSQGGQVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNG"
BASE COUNT          899 a          705 c         1097 g          915 t
ORIGIN      
        1 taatgagtaa aaaattcaca aagacacttc tgtcatcttc tctgggtggg cttctgcttg
       61 ggatcagctc aggtgtttta gctcaggagt tacctcctat caaagattct ggtttgcctt
      121 tttattcagt gctgaaagag gcagatgctt cgtctgttat acacggtagt acttatcgta
      181 tttactataa caaatcctct ggttggggaa caatgcgata tgttctgaac ggaaaagatg
      241 agaccctttt taatttcgat caggagggta atattatagt attgagtaaa gataactcaa
      301 ttgcatatac cgttcatgag cctgtgctaa aagacttcgc ccgtatggca gcaggcctca
      361 gaacgtctga caaagaaaac ggtaagcatc aggttgatga ggaggaggtc cgccggatct
      421 tcaataaagt taataatcta tcaaatacta ttattaatcc tgaagtatta aaacaatcgg
      481 atttcatcgc atcaggacca cgtccggaag gtgacccttt ggttcaggct agtgcggtgg
      541 gcgaattcgc tctggctgtt ggagaaggtg cagcggcgga ggaaaagaat tcaacagctg
      601 ttggttcatg ggctagtgct catgggagac agagtactgc attaggaagt ggggggcagg
      661 cttatgctaa tggttcaacc gcgttgggaa gttattctca tgtggatgat tcatccgttt
      721 atggcgtggc attagggggc agctctgttg tgggtgaaaa tgcccccgaa ggcactgcac
      781 tgggaagcag agcggaagtt tctcatagaa atagtgttgc tttaggggcg agagcgcaca
      841 ctacccgtga taatgaagtt tatatcggct acgtagaaga agcgggcaag gcttataaaa
      901 ctcgtgtgct tggtggcctg agtgacggta ctcgtccctc agatgcagca accgttcgtc
      961 aggttgaccg tgtaaaagac agtgttgagc aactggctca ggatacgaat acccgccttg
     1021 tagtcgaagc gaaaaagtct cgtgaatata cagattccag aacgacagtt ggtgtgaact
     1081 ctgacggaac cctgaccagc gctgatggcg ctacaaaaac gattgctgtt aatgacggtc
     1141 tcgttgctct gtccggcaga acagaccgta ttgatgctgc agttggtagc gttgaccgcc
     1201 gggttactaa aaatacacag gctattcagt ccaatactcg ccagttgcag gaacataatg
     1261 cacgcctgaa cagccagcag cgtcaaattc gtgagaatca cgaagagatg aagcgtgcag
     1321 cagcacagag tgcagcgctg gcggggcttt tccagccgta cagtgtgggg aaattcaacg
     1381 ccactgctgc cctggggggg tacagtgata aacaggctgt tgccgtgggt gttggttacc
     1441 gtttcaatga gcagacagca gcgaaagcag gtgtggcagc gagtgatggc gatgtgtcct
     1501 acaacgtagg tgtgaacttt gagttttaat tgctgacagt gacatgaagc atgacggcag
     1561 gcgtggagtt ttgcctgccg ttttttctca tgtgagcatg aaacaggtga cagcgatgaa
     1621 agtgattctg gcgacccgca accgttatct ggagtatggc ctgcagcaga tgcttgaggg
     1681 gtacaggata atactggcga gggagttttt tacaccagag aacaggaaga gtgtaccggc
     1741 acatgatgag tcatgggtga taatctgtga tgccctcctt ggcaggctga tgtgctgcat
     1801 gtttcaggga cgccgttatc ttcagattga tgcggaagat gttacggggc gcctggagac
     1861 gtacaggaaa atccggaatg gtgagtgggt acataatacg tatgcgcgac ctctgacgat
     1921 gtccgagatg gtggtgatgt ttggatacgt gtaccgggag tcaaagccct gccatcttgc
     1981 ccgtgaaatg gggataaaca cgaagacggt gaacaccttt ctgtatatgg gacttggtaa
     2041 aaatggtctt aagtacagga gtgtgaagca cctggttgga cgtgcgtagc cggaatgcat
     2101 attcgtgagt cgggtaatga gagtggaacg gtggcagtga gaatggaaca gaaaaaaatc
     2161 agatgcggaa agcggaaata cacagcgggt gcgatgtgct tcatgctgtt tgtatggctg
     2221 gggggtgtgg tgacattctg tgtgctggcc gctctgataa tgaggtgaaa acggaagaga
     2281 gtttacttcc ttaattcgaa ctttctgata actgagaact tgttaaatgc ctgtgggttt
     2341 atgtatttaa atcaattggt taatggtgat tttatcttta tcagtcaaag ttcgttttag
     2401 tatgatgatt gtgcccgctg tttattgttg tccgggagaa aatacaggga atccagggga
     2461 tgaacaaaat atactcactc agagttaatg acagaaaaga actggttgct gtaccggaaa
     2521 cggtgggggg caggaaaaag tcgtccggaa ttcccggtgc ggggatgctt tcccgtcttc
     2581 ttctggcctc aggagccgtt gccggcgttc tgttttcttc tcctgcgctg gcatccgttg
     2641 tcgggaatac tctgccatgg cagacctaca gggattttgc tgaaaacaaa ggggcgtttc
     2701 atgccggggc cacaaatatt ccgctttatg gtaagaatgg ggctgtcagt gggcggctgg
     2761 acaaggctcc gatgatggat ttcagtgttg tggaccagat actgggggtg gcaacactga
     2821 tttctccgca gtatgtggcg ggggttaagc ataacggcag ctacaatacg gtgcgttttg
     2881 gttatgcgga cgatacgaca tatcgccttg ttgaccgtaa tgagcactgg cgtgattttc
     2941 atactccccg gctgaacaaa ctggtgacag aagttgcccc ggtgagcgtc acggatgccg
     3001 gaaccggcaa gggggtatac cagaaccgca gtcgttatcc ggtgttttat cgtatgggga
     3061 gtggaactca gtacaccgga gctgcttccg gtgcgctgac cagaatagcg gatgcttacg
     3121 tctggaggac tggcgggacg gtgggaagtc cggtcatttc tgactggagt ctggtgtcaa
     3181 acccgggaaa tctttaccag ggtgttaatg gtccgctggc gtcttacggg acatcaggag
     3241 acagtggctc accgcttttt gcctgggatg cggtgaagaa gcagtgggta tttgttgctg
     3301 tacttcatgg ttatgccggt gagaacggga agacgaactg gtttacggtg attcctgcag
     3361 gggatgtgaa taacacgata aagcaggaca attcaggcac agttgtgccg gcagttgccg
     3421 gtggtgacat tgtgtggaag tacagtcagg gaagcggaga gggaaccctt tctcagggcg
     3481 ggcaggtatg gaagatgaac ggcttcaggg gagggagtct gaatgacggg aaagatatca
     3541 cctttggggg taagggcacg gttgttctga aggatgatgt tgtccagggg gcgggttcgc
     3601 tcacgtttaa tggtga
//
LOCUS       DAZKFU020000348         3601 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZKFU020000348 DAZKFU020000000
VERSION     DAZKFU020000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.350"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..218
                     /locus_tag="ACHOQQ_RS22915"
                     /old_locus_tag="pgaptmp_004583"
     CDS             <1..218
                     /locus_tag="ACHOQQ_RS22915"
                     /old_locus_tag="pgaptmp_004583"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472735.1"
                     /translation="KNNTSKPPDQQVSHRLSEQQNRFAGGWSHCVTPTTITSVFSRTV
                     VNHKIGTHAGGAGPHDFLFDESENGER"
     gene            509..703
                     /locus_tag="ACHOQQ_RS22920"
                     /old_locus_tag="pgaptmp_004584"
     CDS             509..703
                     /locus_tag="ACHOQQ_RS22920"
                     /old_locus_tag="pgaptmp_004584"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGU4472736.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            complement(2259..3275)
                     /locus_tag="ACHOQQ_RS22925"
                     /old_locus_tag="pgaptmp_004585"
     CDS             complement(2259..3275)
                     /locus_tag="ACHOQQ_RS22925"
                     /old_locus_tag="pgaptmp_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HGU4472737.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYESDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLAERPAARWLFMTLTVKNPPVEDTRETLIRMNAAWNRLVRRKEFRSVLGWLRTTE
                     ITYGKVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKRPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLIGADIPAELDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
BASE COUNT         1016 a          913 c          772 g          900 t
ORIGIN      
        1 aaaaaaacaa cacatccaaa ccacctgatc agcaggtttc tcaccgactt tctgaacaac
       61 aaaaccgctt tgcaggtggt tggtcccact gcgtgacacc aaccaccatc accagtgttt
      121 tttctcgtac cgtcgtcaat cacaaaatcg gaacccacgc cggaggcgcg ggaccccacg
      181 attttctgtt tgacgagtca gaaaacggcg aacgctgaca gatttcaatc tggctgcgtg
      241 agacgttggc ggggtgtcgg ggcgcagccc tgaccaggcg gtatttgtct gattgcgtgt
      301 gcgcggtccg acaaatacac atcctgtccc gtttttcgca cggtttttat tgattaccgg
      361 acagatgaac ccggattgcc atgttgcgaa aatcacgtta aaacgattac gagcgtgcag
      421 ttgggctgat atatcaacag tcatgccaat atgtattaac ccgacaggcc gctctttttt
      481 aattcaaata aaaatataaa ttacgagtat gaatcaggac aaagcaattt atttagaaaa
      541 taaatatttt tcacccgcaa acgaagctgt cagactggag cgtgaaggcg attacattgg
      601 agcatggaaa aaatggaatg atgcgcttca ttatgccaaa ggagaaaata tagactggtg
      661 tatagccaga gctgaatatt gtaaaaagac aggccggttt taattctgac ggggtttcaa
      721 aggggcgtcc cctttggcac gtcgacgtat caaaatgcgc cagcattttg tatacagtcg
      781 gtaacgtgcc tccaggattt accatcaaaa acgcccctgt agttaatcct gaatcaagat
      841 ataactaaga tataattagc gtataactaa ggcataacta aagaataact aacttagtgt
      901 gcatataatt aagatataac taatatataa ctaatgtata actaacggtt caccaacgga
      961 tcgcatgtag tagacgctac ataaaaaatc ctgaaatgga ggattcgtca taaagagatt
     1021 cctgttttta gcgttctgat gcaggatagg gcaaacaccg caaaatgacg tctttggtat
     1081 gttttgtgat gtttgcttct ggcatctgtt atctgtttta ttgggtgttt ttatgagtgt
     1141 tgatgtaatt aaacaaattc agagtataca ggacaaagga attattattt attcaaaata
     1201 cagagctaac gaatttaaca gggatgatgt atacagagag agttacagcc tgattattga
     1261 gtttaatcat cttattgctg aacatgccgt tcatgatgaa aaactgatta accatactgt
     1321 ctgtattctg aatgaattac gaagcatagc gataaattaa aaaatccccc tggattacag
     1381 ggggaagcca tcagagtttg agtaatgaga aatattattt tggagagagc accacaaatg
     1441 cgctcttttc caatctgccg caaccatcag caaaagacaa tcagaacagg cgaaaaaatc
     1501 cccctatatg gcaagggggt gtcaatgagg acatggcaat tgtgagtttt gtgtgtttta
     1561 tgtgttgcag tttgcaacgt cagaaatttg ccgcagttat gcgcgaaaaa caatcctgat
     1621 tccgggacag gatgtgtaat cgtataaccg cgcacgcacg gctatacgat taccacctgg
     1681 tcagggcttt gcccgacacc ctcgacaccg ttcccgcaat acgatagaac atcagggcgc
     1741 gtttccgggt aaacgcatgt taatgaccgg gaattcattg attagggacc ggagacgcgc
     1801 tacaaaactc ctgcgcgttt ctggcatcaa ctgaccatgg ggatggtttg gcagtagcag
     1861 caagcgccag aaacgcgctc aaaatcatca gatgtacagc gaacaaaaaa ttgtcatctg
     1921 caaaaaatca cgttaaaaca atcgcgagca ccttcctgtc tccttcaatt acggtcaaac
     1981 attttgcatc tgtcgggaag ttgctcacct ccataattca atttctcatt gacaataatt
     2041 ccggtgatat cccctctttt gaggggcttt ttttacccac acaccacaaa cagcagccat
     2101 accatcgtca aaaacgcgct caaaacgtct ctgacgccct gaaccaacca aaacatacaa
     2161 aaccattgcc caatgcaaaa aagtgcgtta gaaagcgtct gagcgcgttt ctgacgtctt
     2221 ttttgttgct gtgtcgctac tcgttgtcag attcgcgctc aatccttcat cctgtcagct
     2281 tcaggattcc gcttgtaacg tttttccgta cgtccccagt caaacgccag ccagaaggct
     2341 tgctcctcga actcgtccag ttctgccgga atatctgccc cgatcaaatc ctcgtccgtc
     2401 tccggtttat cgactttcag aacctctttg agacgaccgc cggttgccac catgcgaagc
     2461 ccataggtct gccgcgccag ctccagaaac cacgccggat cacgcacaac ttccgaagat
     2521 ttcaccgtgt atttcatcgt ctcaatgacg cctgactcca gcgccgcacg atgcatatca
     2581 ggcagggtta caccatccgg ccgtttccag ccctgtttcg gttttaccac ccgaatatcc
     2641 acacccgcct cataatcgac acgaaggcat tcactccaga tctccaccca gcgagcatgt
     2701 ttcacataac cattaccgga caacatcgaa ggggggacca tcatcagaac gtgaaaatgc
     2761 ggatggcaac atcctggcac cttcccgtaa gtaatttccg tcgtacgtaa ccagccaaga
     2821 actgacctga attctttacg ccgaaccaac ctgttccacg ccgcattcat ccgaatgagt
     2881 gtttcgcggg tatcttccac agggggattt ttgacagtca gtgtcatgaa caaccagcgg
     2941 gccgcaggac gttcggccag cagctttggc aaggcctccc agaggcgacg catccagcgg
     3001 agagagcgac gacgactaca caccggacaa aacggaacat gacaaaactc ccccttccga
     3061 agggccagct tcatttcgcc cgtcaggcgg gacaccttcg gcgcaaacaa caaccgacgg
     3121 gaacaatcaa acatccgttc tccacgagag gaaaacatcg aatcgctttc gtaaatctgc
     3181 gccaccctgt cagccatcac tcgatcactg tcccattttg tatcccgcgt gctgtaatcg
     3241 cgtaacgaga gtaccggact gtctggattt ttcatttgca ccccccaaga actgggcaga
     3301 tgcggaaatt ttgtgttagc atttttctgt cacctgtgac tctgttgccg aacaacgaat
     3361 tccaaactgg tgatcgtccc ctggagtgtt accgcactcg caggggaacc ccctcaaaat
     3421 cgttcatttt ttaaccagaa gtttttatct cttttccacc catttttacc gctcaaagcc
     3481 gcgccaccac tgggctaagc caaaataaca catccttgag ttgttgccta tagtataaat
     3541 acaaggatcg cgcgcgcgat cgcccaccca aaatgcgcgt tttttgagcg ttcccgagaa
     3601 c
//
LOCUS       DAZKFU020000349         3588 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZKFU020000349 DAZKFU020000000
VERSION     DAZKFU020000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.351"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..830
                     /gene="yibB"
                     /locus_tag="ACHOQQ_RS22930"
                     /old_locus_tag="pgaptmp_004586"
     CDS             <1..830
                     /gene="yibB"
                     /locus_tag="ACHOQQ_RS22930"
                     /old_locus_tag="pgaptmp_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HGU4472738.1"
                     /translation="YFDIGRGDWTANKGFREKLARSVDVYFSYFERLAVLENEMIIFT
                     SPDLKPRVEAIRNGKPTTVIAIDIKKKFRHIRSRIEKIQKDESFTNRLEPRQLKNPEY
                     WSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDENK
                     MHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQYKWKEFYKLVLESQKITLNN
                     NIVDDDQGIFVMCYYKRSGLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
     gene            1105..2301
                     /gene="kbl"
                     /locus_tag="ACHOQQ_RS22935"
                     /old_locus_tag="pgaptmp_004587"
     CDS             1105..2301
                     /gene="kbl"
                     /locus_tag="ACHOQQ_RS22935"
                     /old_locus_tag="pgaptmp_004587"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGU4472739.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2311..3336
                     /gene="tdh"
                     /locus_tag="ACHOQQ_RS22940"
                     /old_locus_tag="pgaptmp_004588"
     CDS             2311..3336
                     /gene="tdh"
                     /locus_tag="ACHOQQ_RS22940"
                     /old_locus_tag="pgaptmp_004588"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGU4472740.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          906 a          762 c          937 g          983 t
ORIGIN      
        1 catattttga cattggtaga ggggattgga ctgctaataa agggttccgt gaaaaactag
       61 ctcgttcagt tgatgtttat tttagttact ttgagcgtct ggccgtactt gaaaatgaga
      121 tgattatttt cacttctccc gatttgaaac caagggttga agcgattcgc aacggaaaac
      181 caacaacggt tatcgctatc gatataaaaa agaaatttag acatatcaga agtcgtatcg
      241 aaaaaattca aaaagatgag tcgtttacaa atagacttga gcctcgacaa ttaaaaaacc
      301 cagagtactg gtcaccagag tatgttttgg tatgtaacct taaggcctat tttgtaaata
      361 aagctatcaa catgggctta gtgaaaacgc ctcttgttgc gtggatagat tttggctatt
      421 gccgtaagcc aaatgtgact cgaggattga aaatttggga tttcccattt gatgaaaata
      481 agatgcatct tttcactata aaaaaagggc ttactgttac atctcaacaa caggcttttg
      541 atttcatgat tggcaatcat gtatatatta ttgggggcgc aattgttggt tcacagtata
      601 aatggaaaga attctataaa ttagttctgg aaagtcaaaa aataacacta aacaataaca
      661 ttgtggacga tgaccagggc atttttgtca tgtgctatta caaacgttcc ggtctcttca
      721 acctaaacta tctggggaga ggaaaatggt tcgatttgtt tcgctgcttc aggagtaaca
      781 ctttaggggc aaaaatgcag gcgctgagaa ttttcctttc aagaaaatag gtttcccgcc
      841 attagtgggt agcgttttga tgccaactga gataggaaaa atgatgtttt ggcatcattt
      901 tttcttttta tatcaatgca gtaaaatcac tcatcattat gctcagccaa aactgtacag
      961 tttttctact atctgtaggt gttgagagac atcaagatac acgttattta tcctgaattt
     1021 tgcagaagtg ttaacgcgtc atctcgtcgc gacctataag tttgggtaat atgtgctgga
     1081 atttgccctg tctggagaat cgcaatgcgt ggagaatttt atcagcagtt aaccaacgat
     1141 ctggaaaccg cacgggcgga agggttgttt aaagaagagc gcattattac gtctgcgcag
     1201 caagcagata tcactgtggc tgatggaagc cacgtcatta acttttgtgc caataactat
     1261 ctcgggctgg cgaatcatcc ggatctgatt gcggcggcaa aggcgggaat ggattctcac
     1321 ggtttcggca tggcttcggt gcgttttatt tgcggcactc aggacagcca taaagagctt
     1381 gaacaaaaac tggcggcatt cctggggatg gaagatgcga ttctctactc ttcctgcttt
     1441 gatgctaacg gtggcctgtt tgaaacgctg ctgggcgcgg aagacgccat tatctccgac
     1501 gcactgaacc acgcgtctat tattgatggt gtgcgtctgt gtaaagctaa acgctatcgc
     1561 tatgccaaca acgatatgca ggagcttgaa gctcgtctga aagaagcgcg tgaagccggt
     1621 gcgcgtcatg tgctgattgc caccgatggt gtgttctcga tggacggcgt gattgccaat
     1681 ctgaagggcg tttgcgatct ggcagataaa tacgatgccc tggtgatggt agacgactcc
     1741 cacgcggtcg gttttgtcgg tgaaaacggt cgtggttccc atgaatactg cgatgtgatg
     1801 ggccgggtcg atattatcac cggtacgctt ggtaaagcgc tgggcggggc ttctggtggt
     1861 tatactgcgg cgcgcaaaga agtggttgag tggctgcgcc agcgttctcg tccgtatctg
     1921 ttctccaact cgctggcacc ggccattgtt gccgcatcca tcaaagtact ggagatggtc
     1981 gaagcgggta gcgagctgcg tgaccgtctg tgggcgaacg cgcgtcagtt ccgtgagcaa
     2041 atgtcggcgg cgggctttac cctggcggga gccgatcacg ccattattcc ggtcatgctt
     2101 ggtgatgcgg tagtggcgca gaaatttgcc cgtgagctgc aaaaagaggg catttacgtc
     2161 accggtttct tctatccggt cgttccgaaa ggtcaggcgc gtattcgtac ccagatgtct
     2221 gcggcgcata cccctgagca aattacgcgt gcagtagaag cattcacgcg tattggtaaa
     2281 caactgggcg ttatcgcctg aggatgtgag atgaaagcgt tatccaaact gaaagcggaa
     2341 gagggcatct ggatgaccga cgttcctgta ccggaactcg ggcataacga tctgctgatt
     2401 aaaatccgta aaacagccat ctgcgggact gacgttcaca tctataactg ggatgagtgg
     2461 tcgcaaaaaa ccatcccggt gccgatggtc gtgggccatg aatatgtcgg tgaagtggta
     2521 ggtattggtc aggaagtgaa aggcttcaag attggcgatc gcgtttctgg cgaaggtcat
     2581 atcacttgcg gtcactgtcg caactgtcgt ggcggtcgta cccatctgtg ccgcaacacg
     2641 atcggcgtcg gcgttaaccg tccgggctgc ttcgccgaat atctggtgat tccggcgttc
     2701 aacgctttca aaattcccga caatatctcc gacgacctgg cttccatttt tgatcccttc
     2761 ggtaacgccg tgcataccgc gctgtcgttc gatctggttg gcgaggatgt gctggtctct
     2821 ggtgcaggtc cgataggtat tatggctgcg gcggtggcga aacacgttgg tgcacgcaat
     2881 gtggtgatca ctgatgttaa tgaatatcgt ctcgaactgg cgcgcaaaat gggtatcacc
     2941 cgtgcggtca acgtcgctaa agaaaacctt aatgatgtga tggcagaact gggcatgacc
     3001 gaaggctttg atgtcggtct ggaaatgtcc ggtgcgccgc cagcgtttcg taccatgctc
     3061 gacaccatga accacggcgg tcgtattgcg atgctgggta ttccaccttc tgacatgtcg
     3121 atagactgga caaaagtgat ctttaagggc ttgttcatta aaggcattta cggtcgtgag
     3181 atgtttgaaa cctggtacaa gatggcggcg ctgattcagt ctggcctcga tctctcgccg
     3241 atcattaccc atcgtttctc tatcgatgat ttccagaagg gctttgacgc tatgcgttcg
     3301 ggccagtccg ggaaagttat tctgagctgg gattaacacg aacaagggct ggtattccag
     3361 cccttttatc tgaggataat ctgttaaata tgtaaaatcc tgtcagtgta ataaagagtt
     3421 cgtaattgtg ctgatctctt atatagctgc tctcattatc tctctaccct gaagtgactc
     3481 tctcacctgt aaaaagaata tctcacaggc ttaatagttt cttaatacaa agcctgtaaa
     3541 acgtcaggat aacttcagag gtcgtcggta atttatgatg aacagcac
//
LOCUS       DAZKFU020000350         3569 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZKFU020000350 DAZKFU020000000
VERSION     DAZKFU020000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.352"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2060
                     /locus_tag="ACHOQQ_RS22945"
                     /old_locus_tag="pgaptmp_004589"
     CDS             <1..2060
                     /locus_tag="ACHOQQ_RS22945"
                     /old_locus_tag="pgaptmp_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4472741.1"
                     /translation="AEAVGAVRSTLGGLGAVSENELTRITRKMLDMQAVMGGDIPQHI
                     QAAAIMIKNGLARSSDEAVDLMVAGMQRMGPVMRDELPEILHEYSTHFRNMGLTGSET
                     MTLLADMAQQGKFALDKTGDAIKEFSIRGSDMSKASAEAYALIGLDAARMSSAVASGG
                     TAARAALQKTASGLLQIVDPAERANAAIALFGTPVEDLSVDQIPAFLGALARVSDRMA
                     DVSGVADRMGQTLRDNLSGDVARLTGTFSALRSGIIGEITDDLRGLVRMATAWGERLQ
                     AWVNAHPELVRMIALTTGAVLALTTAMGALSVAAWMVVAPLLKLRLGLALLRAIPAGL
                     SVAGTLFGGLTGVISAVGAALGALTWPVVVAVGALVGGALLIWRYWDRVRAFVSGFIE
                     GVQSALAPFMATASRFAPLLDLVSGALSRILGWFKSLLTPVSSTRDLLEQCTSAGQTF
                     GSTLAGALATVFSPLKALIDGLAWVLEKLGVLPGEAERVKRKLDEARKTPVAWEWDPE
                     QKKMVQKAWHWSPPGGASVPSGASGTQPPPVALPAGIAPDGNRNVLRHLKGIEGNTGG
                     LLAENRKRIGPGDIVFKHLPRALAVRGQWHEPVVAPSSGGMRALPQEEIQVQQWPTAR
                     PAPRMHQQETFASAFQGEIHVHLHGVSSHNPRELARMVGDAVRAEMNRMARAGTGSFR
                     DRD"
     gene            2071..2562
                     /locus_tag="ACHOQQ_RS22950"
                     /old_locus_tag="pgaptmp_004590"
     CDS             2071..2562
                     /locus_tag="ACHOQQ_RS22950"
                     /old_locus_tag="pgaptmp_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4472742.1"
                     /translation="MMMIYGMFVFQLSTLPHQQVEQSKRWRHVSNERVNRSSSWQYIG
                     SGEDHITMSGVLCPEITGGEVSLTALNTLAYTGLPWPLIDGVGQIYGMYVITEIRTTR
                     QELDRYGMARKIEFTVSFQRCNEDLRERLQSGSVGDLMAGLKNSASSALSSASSALSG
                     LML"
     gene            complement(2573..3160)
                     /locus_tag="ACHOQQ_RS22955"
                     /old_locus_tag="pgaptmp_004591"
     CDS             complement(2573..3160)
                     /locus_tag="ACHOQQ_RS22955"
                     /old_locus_tag="pgaptmp_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4472743.1"
                     /translation="MHTIKSARNAAYNRDGTIDCEIQFEGEESWLPYTATEYDNSPHG
                     KKLWGELKAGNHGPVSEYQENDTDRENARRLKNDEINAWRNAMEAASYTFEHNGRKWD
                     YGKSTQARLEPSVAAAKAGILPLDFFWTDAQNNDVPVSADELIALSDAAAKALFAKGM
                     EIHIRQRNMKKEIAALNDTAAILDYKVGWGKEAEA"
     gene            complement(3172..>3569)
                     /locus_tag="ACHOQQ_RS22960"
                     /old_locus_tag="pgaptmp_004592"
     CDS             complement(3172..>3569)
                     /locus_tag="ACHOQQ_RS22960"
                     /old_locus_tag="pgaptmp_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4472744.1"
                     /translation="SSATDSDSEVLAATPKAVKTAMTAAENAGQKARQALEAAQAKTQ
                     RTLWDVGSFILAATDAEGVYPARINIAPGDTRPGSKLRLMSMTNSDFEISFYESMQNW
                     NTNRRPPGTWRACADIYAGAGIGIFERIA"
BASE COUNT          650 a          927 c         1127 g          865 t
ORIGIN      
        1 cggctgaagc tgtgggtgct gtccgctcca ctctgggtgg gctgggggcg gttagtgaga
       61 atgaactgac ccgaatcacc cgaaaaatgc tggatatgca ggccgtgatg gggggcgata
      121 tcccgcagca cattcaggcg gctgccatca tgatcaaaaa cggtcttgca cgcagcagtg
      181 acgaggcggt tgatctgatg gtggcgggca tgcagcgcat ggggccggtt atgcgcgatg
      241 agctgcctga gatccttcac gagtattcga cccattttcg taacatgggg ctgacagggt
      301 cggaaaccat gacgctcctt gcggatatgg cgcagcaggg gaagtttgcg ctggataaaa
      361 ccggcgatgc catcaaggag ttcagtattc gcggctccga tatgtcaaag gcgagtgctg
      421 aagcgtatgc gcttatcggg ctggatgcag ccaggatgtc atcggctgta gccagtggtg
      481 gaacggctgc acgggctgcg ctgcagaaaa cagcctcggg tctgttgcag atcgtcgatc
      541 cggctgagcg ggccaatgcg gccatcgcac tttttggtac accggttgaa gatctgtctg
      601 tcgatcaaat ccctgccttt cttggcgcgc tggcgcgggt gtctgaccgt atggctgacg
      661 tgtctggtgt ggcggaccgg atggggcaaa ccctgcgtga caatctgagt ggtgatgtgg
      721 cgcggcttac cgggacgttc agcgcgctga gaagtgggat tatcggggag attacggacg
      781 atctgcgtgg gcttgtccgg atggccacag cgtggggtga gcgtctgcag gcgtgggtga
      841 atgcgcatcc ggaactggtg cgaatgattg cgctgacgac gggagcggtg ctggcgttaa
      901 ccacagcgat gggggcgttg tccgttgcgg cctggatggt tgtcgcaccg ttactgaaac
      961 tgcgtctggg gcttgccctg cttcgcgcga tcccggccgg actctctgtt gccggtacgc
     1021 tgtttggcgg actgacgggt gtgatttcag ccgtgggggc tgcgctgggg gcgctgacct
     1081 ggccggttgt ggtggcggtg ggagccctgg tcggtggtgc tctgctgatc tggcgctact
     1141 gggatcgggt cagggcgttt gtgagcggat ttattgaagg ggtacagtca gcactggcgc
     1201 ccttcatggc gacagcctcc cgttttgcgc cgctgctgga tctggtttca ggggcactgt
     1261 cccgtattct gggatggttt aaatccctgt taacgccagt ctcctccacg cgtgatttgc
     1321 ttgagcagtg cacctctgcc gggcagacgt tcgggagcac tctggccggt gcgttagcca
     1381 cggttttttc gcccctgaaa gctctgattg acggtctggc gtgggtgctg gaaaaactcg
     1441 gtgttctgcc cggtgaagct gagcgggtta agcgaaaact ggatgaggcc cgtaaaacgc
     1501 cggtggcatg ggagtgggat ccggaacaga aaaaaatggt tcagaaggcg tggcactggt
     1561 cgcctcctgg tggggccagt gtgccttccg gcgcctccgg tacacagcct ccgccggtgg
     1621 cgctccctgc ggggatagcg ccggacggta accgtaacgt tctccgtcac ctgaaaggca
     1681 ttgaggggaa taccggtggt ctgctggctg aaaaccgtaa acgtatcgga cccggagata
     1741 ttgtgtttaa acatcttcca cgggcgctgg cggtccgggg gcagtggcat gaacctgtcg
     1801 ttgcgccgtc ttccggcgga atgcgcgcgc tgccacagga ggagattcag gtgcagcaat
     1861 ggccgacggc acgcccggcg ccacgaatgc accagcagga aaccttcgcc agcgcctttc
     1921 agggggagat acatgtgcat cttcacgggg tgagttcgca taatccccgg gaactggcgc
     1981 gcatggttgg tgacgctgtc agggcggaaa tgaacagaat ggcacgtgcc ggaaccggca
     2041 gcttccgcga tcgcgactga ggtgaaaaag atgatgatga tttatgggat gtttgtgttt
     2101 cagttaagta ccctgccgca tcagcaggtg gaacagagta aacgctggcg acacgtcagt
     2161 aatgaacggg ttaaccgttc atcctcctgg cagtatattg gctctggtga agatcacatc
     2221 acgatgtccg gtgtgctctg tccggaaatc accggcgggg aggtgtcact gactgccctg
     2281 aacaccctgg cctacacagg attaccctgg ccactgattg atggtgtcgg acagatttac
     2341 gggatgtatg tcattacgga aatcaggacc acccgtcagg agctggatcg ctatggcatg
     2401 gcccgcaaaa ttgagttcac tgtcagcttt cagcgatgca atgaagatct gcgtgagcgt
     2461 cttcagtcgg gctcggtggg tgatctgatg gcaggattaa aaaacagcgc gtcgtcggcc
     2521 ctgtcgtcgg cgtcctctgc gctgtctggt ctcatgttgt gaaggtcact gttcaggctt
     2581 cggcttcttt tccccagcca accttatagt ccagaatcgc cgccgtatcg ttcagggcgg
     2641 cgatctcttt tttcatgtta cgctggcgaa tgtgaatttc catccctttg gcaaacaggg
     2701 cttttgcggc tgcatcgctg agggcaatca actcatcagc agaaacagga acatcgttgt
     2761 tctgtgcatc agtccagaaa aaatccagcg gcaggatccc cgcttttgcg gcagcaacgg
     2821 aaggctcaag gcgggcctgc gttgacttcc cgtagtccca tttcctgccg ttatgttcaa
     2881 aggtgtaact cgccgcttcc atcgcattac gccaggcgtt gatttcgtcg tttttcaggc
     2941 gtctggcgtt ttccctgtca gtgtcgtttt cctggtattc agatacaggg ccgtgatttc
     3001 ctgctttcag ttcgccccag agttttttgc cgtgtggcga gttgtcatat tcggttgccg
     3061 tatagggcag ccagctctcc tccccttcaa actggatctc acagtcgata gtgccgtcac
     3121 ggttataagc ggcatttctt gctgatttga ttgtgtgcat gagtctctcc gtcaggcaat
     3181 gcgctcaaat attccgattc ctgcgcctgc ataaatgtca gcgcaggccc gccacgtgcc
     3241 cggtggcctg cggtttgtgt tccagttttg catggattca taaaaactga tttcgaagtc
     3301 ggaattagtc atgctcatca gtctgagttt actgcccggg cgggtatctc cgggcgctat
     3361 attaattctg gcaggatata ccccctcggc atccgttgcc gccagaataa agctgcccac
     3421 atcccagagt gtccgctgtg tttttgcctg tgcagcctcc agtgcttgcc gtgctttctg
     3481 cccggcgttt tctgctgccg tcatggccgt cttcacagct tttggtgtgg cagccagcac
     3541 ttcactgtca ctgtccgttg cgctgctta
//
LOCUS       DAZKFU020000351         3518 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZKFU020000351 DAZKFU020000000
VERSION     DAZKFU020000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.353"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..614)
                     /gene="mobB"
                     /locus_tag="ACHOQQ_RS22965"
                     /old_locus_tag="pgaptmp_004593"
     CDS             complement(87..614)
                     /gene="mobB"
                     /locus_tag="ACHOQQ_RS22965"
                     /old_locus_tag="pgaptmp_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGU4472745.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
     gene            complement(596..1180)
                     /gene="mobA"
                     /locus_tag="ACHOQQ_RS22970"
                     /old_locus_tag="pgaptmp_004594"
     CDS             complement(596..1180)
                     /gene="mobA"
                     /locus_tag="ACHOQQ_RS22970"
                     /old_locus_tag="pgaptmp_004594"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGU4472746.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1250..1519
                     /gene="yihD"
                     /locus_tag="ACHOQQ_RS22975"
                     /old_locus_tag="pgaptmp_004595"
     CDS             1250..1519
                     /gene="yihD"
                     /locus_tag="ACHOQQ_RS22975"
                     /old_locus_tag="pgaptmp_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGU4472747.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1596..2582
                     /gene="srkA"
                     /locus_tag="ACHOQQ_RS22980"
                     /old_locus_tag="pgaptmp_004596"
     CDS             1596..2582
                     /gene="srkA"
                     /locus_tag="ACHOQQ_RS22980"
                     /old_locus_tag="pgaptmp_004596"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGU4472748.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFNHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2599..3225
                     /gene="dsbA"
                     /locus_tag="ACHOQQ_RS22985"
                     /old_locus_tag="pgaptmp_004597"
     CDS             2599..3225
                     /gene="dsbA"
                     /locus_tag="ACHOQQ_RS22985"
                     /old_locus_tag="pgaptmp_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGU4472749.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            3380..>3518
                     /locus_tag="ACHOQQ_RS22990"
                     /old_locus_tag="pgaptmp_004598"
     CDS             3380..>3518
                     /locus_tag="ACHOQQ_RS22990"
                     /old_locus_tag="pgaptmp_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HGU4472750.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KG"
BASE COUNT          909 a          824 c          850 g          935 t
ORIGIN      
        1 aggccatcct gacggatggc ctttttgcgt tggtgcaaaa aaatgccgga tgcgacgctg
       61 gcgcgtctgc tccagcctac tcttgcttat ccgtcctgat tttgcatcca ctccaccaca
      121 aaatcagcca gcccctcaac atcattaata tccagtaatg cgacatcaag attaagcggc
      181 acatcactgg ctacagcaat aacatgcctg tctatcacta attcttcagg tcgatgtccg
      241 gctccatcgc gaaacagcac aatctttgcg atctcttcat gcttaaaccc ttcgaccaga
      301 atcaaatcca gctttgaggt atccattcga cttgcgagaa actgtagatc cagctcttct
      361 tcgtctggtg tttccgtcat caaggcccat cgttgctggc tggcaacgat ggtttgtgcc
      421 gcgccagcct tgcgcagctc atagctatct ttgcctggct tatcaacatc catatcatgg
      481 tgcgtatgct taatcagccc tggacggatc cctctggcgc ataatgccgg gatcagtttt
      541 ttcaaaagcg tagtttttcc ggtaccactc cacgcggcaa aggcgagtaa cggtatcatc
      601 gtttttcctg ccatcgggca agctcctctg gcgtattcac gttaacaaat gcatctttat
      661 gatcgctgaa atcaaccgca tgaccgccag ccagacgcat aaatgccatt acccggcgtt
      721 ctcctgcttg cagatattct agtaataaag gctcaatagc gcggtttacc agagcaatag
      781 tcgggtgatc gcgttcaccg tcatggaccc acacgacagg cgcatctttg cgctgatgat
      841 taagccgggc ggctaaatca tggggaatgt aaggcgtatc gcacggacaa aacaaaaacc
      901 actcaccagc ttcctgctgc attactgaaa gcatccctgc cagagggcct gggtaatccg
      961 ccagtgaatc ttcaatcact ttcagaccgc ctgcctgata gatttcctga tgacgattag
     1021 cattaaccac gacgtgagag agctgcgtca taagcgcgtc agcgacatgt tgccataatg
     1081 gtttgccgtt taattcaagc aatcctttat ctacgccgcc cattcgtctg gctttaccgc
     1141 ctgccagcac aacgcctgtt atcgtcgtca tcagattcac cgatatcgcc tcttttattg
     1201 tgggattgac cctgctaacg tgtctgtctc aagagtaagg agcatcacta tgaaatgtaa
     1261 acgtctgaat gaagttattg aactcctcca gccagcctgg caaaaagagc cagaccttaa
     1321 cctgctgcaa tttttgcaga aactggcgaa agagtcaggt tttgacggcg aactggcgga
     1381 tttgacggat gacattctga tctatcacct gaaaatgcgt gattccgcga aagatgcggt
     1441 gatcccgggt ttgcagaaag attatgaaga agacttcaaa acggcgctgt tacgcgctcg
     1501 cggcgtaatt aaagagtaaa agcttgtaag cggcgccacc aaaatcatcg tgaaatgata
     1561 tccttcatca ttcgtaatgt tttccggatg atgggatgaa caacagcgct tttactttcc
     1621 agacactaca cccggatacc atcatggatg ctctgtttga gcaagggatc cgggtggatt
     1681 ccggtcttac cccgcttaac agctatgaaa accgtgtcta tcaatttcag gacgaagatc
     1741 gtcgacgttt tgtcgtcaaa ttttatcgcc ctgaacgttg gacagccgat caaatcctcg
     1801 aagaacatca atttgcgttg cagctggtaa atgatgaagt cccggtcgca gcacctgtgg
     1861 cctttaacgg tcagacttta ttgaatcatc agggatttta tttcgctgtt tttccaagcg
     1921 tcggtggtcg ccagttcgaa gctgataata tcgatcagat ggaagcggtt gggcgttatt
     1981 tagggcgtat gcaccagacg gggcgcaaac agctttttaa ccatcgcccg accatcggtc
     2041 tgaatgaata tctcattgag ccacgcaagc tgtttgagga cgctacactg ataccttccg
     2101 ggttgaaagc ggcattcctg aaagcgacag atgagctgat cgccgccgtt acagcacact
     2161 ggcgggaaga tttcaccgtt ctgcggctac atggagactg ccacgccggg aatattctct
     2221 ggcgcgatgg cccaatgttt gttgatctgg atgatgcacg taatggtcca gctattcagg
     2281 atttgtggat gttgctcaat ggcgataaag ccgagcagcg gatgcaactg gaaactatta
     2341 ttgaagctta tgaagaattt agcgagttcg acaccgctga aatcggactg attgaacctt
     2401 tacgcgccat gcgtttggtt tattatcttg cctggctaat gcggcgttgg gctgatcccg
     2461 cgttcccgaa aaatttcccg tggttaaccg gggaagatta ctggctacga cagacggcga
     2521 cttttataga acaggcaaaa gttctacaag aacccccttt gcaattaaca cctatgtatt
     2581 aatcggagag agtagatcat gaaaaagatt tggctggcgc tggctggttt agttttagcg
     2641 tttagcgcat cggcggcgca gtatgaagat ggtaaacagt acactaccct ggaaaaaccg
     2701 gtagctggcg cgccgcaagt gctggagttt ttctctttct tctgcccgca ctgctatcag
     2761 tttgaagaag tactgcatat ttctgataat gtgaagaaaa aactgccgga aggcgtgaag
     2821 atgactaaat accacgtcaa cttcatgggt ggtgacctgg gcaaagatct gactcaggca
     2881 tgggctgtgg cgatggcgct gggcgtggaa gacaaagtga ctgttccgct gtttgaaggc
     2941 gtacagaaaa cccagaccat tcgttctgct tctgatatcc gcgatgtatt tatcaacgca
     3001 ggtattaaag gtgaagagta cgacgcggcg tggaacagct tcgtggtgaa atctctggtc
     3061 gctcagcagg aaaaagctgc agctgacgtg caattgcgtg gtgttccggc gatgtttgtt
     3121 aacggtaaat atcagctgaa tccgcagggt atggatacca gcaatatgga tgtttttgtt
     3181 cagcagtatg ctgatacagt gaaatatctg tccgagaaaa aataattcaa tgtaaattac
     3241 ataaagcccg tgaatattca cgggcttttt ttattattta ataaatataa atacattctg
     3301 ataatgcatc ctgccgctgg acattatttc agcataagtg gatattcaaa gttttgctgt
     3361 tttatcaggg aatatttata tgatacgtaa gtcagctaca ggtgttattg ttgcgttagc
     3421 cgtaatctgg ggtggtggca catggtacac aggtacgcaa attcagcctg gtgtcgaaaa
     3481 atttattaaa gattttaacg atgctaaaaa gaaaggtg
//
LOCUS       DAZKFU020000352         3464 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZKFU020000352 DAZKFU020000000
VERSION     DAZKFU020000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.354"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            136..462
                     /locus_tag="ACHOQQ_RS22995"
                     /old_locus_tag="pgaptmp_004599"
     CDS             136..462
                     /locus_tag="ACHOQQ_RS22995"
                     /old_locus_tag="pgaptmp_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HGU4472751.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(594..794)
                     /locus_tag="ACHOQQ_RS23000"
                     /old_locus_tag="pgaptmp_004600"
     CDS             complement(594..794)
                     /locus_tag="ACHOQQ_RS23000"
                     /old_locus_tag="pgaptmp_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4472752.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            836..1201
                     /locus_tag="ACHOQQ_RS23005"
                     /old_locus_tag="pgaptmp_004601"
     CDS             836..1201
                     /locus_tag="ACHOQQ_RS23005"
                     /old_locus_tag="pgaptmp_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4472753.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            1484..2041
                     /locus_tag="ACHOQQ_RS23010"
                     /old_locus_tag="pgaptmp_004602"
     CDS             1484..2041
                     /locus_tag="ACHOQQ_RS23010"
                     /old_locus_tag="pgaptmp_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4472754.1"
                     /translation="MLTTQKRKFALALMSGKNRTAAAIEAGYSEKSARCKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQACHREVPPVEKKNQVSVPPDDGQSFSPLSG
                     IDYMEDGLPDPVKAMGRILVENISIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGDAA
                     KKAANRFAVPPPPRLVVNNDNEGNG"
     gene            2038..>3464
                     /locus_tag="ACHOQQ_RS23015"
                     /old_locus_tag="pgaptmp_004603"
     CDS             2038..>3464
                     /locus_tag="ACHOQQ_RS23015"
                     /old_locus_tag="pgaptmp_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4472755.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVASGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQSWKLGGA"
BASE COUNT          788 a          803 c         1040 g          833 t
ORIGIN      
        1 gctaaatgcc gcatggtatc cgctgcatta cagccagtgt gttgagcgac cttattatcc
       61 atgcgcggta ttgtgatggc cgacagggtg gcattgttag aataagactt attcttatct
      121 gtgccgggaa tgaaaatgaa aagaaatctt ccgttaatta ttttgttgtc ttctctggtt
      181 atgggctgta cgcaacataa aacagatatg ccccgacagt tggttaaggc attaccacaa
      241 tatccggcct atgcagcggc aaattatata aagggacggg ttgatgtgaa gtttgatatt
      301 ggtgctgatg gtactgtcac ccgaattgag tttatccgtt cagagccgca ccatctgttt
      361 gatgagcagg ttgtaaaggc gatggcaaaa tggcgatttg agaaggacag gccgcgtaaa
      421 ggcgtgaaga aaacgtttat ctttagtcct tctgcaccct gattatttca tcagaaatta
      481 attatcactc tgttgttatt ctgtacatcc cggctgggta agtcttgttc cgccgggtat
      541 gaagatgaaa tattgttgga ggacagtggg tacctgctcc tgtaaccgaa cgttcatttc
      601 tcgttatttg tcatgctggc cgggcgcagg tgcgttgcat ctgttgccag ccttctccgg
      661 caggcttcaa taacccacgc tgaaaagtta ccggaccctt tatgctcaag ggcgatgttg
      721 atctgttcaa tcatgtgatt ggggaaacgg atattgcggg ttgtggttct gcgggtccgg
      781 tttttcgatg acatatttat ttcctttact gattgcctta tgacggggat tttacatggc
      841 tgagcttcgt acactccaga gcagaatcaa aacactgaat acccgacggg tgaatattct
      901 gaagggtgaa cagcgtcgtg tcagtggcag tgcacgtgtt tccctcaagc gtcatatctg
      961 gctcagggat gccgggcagt gctgtctctg tggtcgtgtg gttgacctct gtgacagtga
     1021 actcgatcac cgaattgcac ttcagttcgg tggtggtaat gaggagacga atctctggac
     1081 gctctgtacc gaatgccatc gacaaaagtc tgctcgtgaa gcggcgggtg gtatgccgga
     1141 cccgacactg ccggaggtgt ccggaggtag tggcagagcg gacgacatca tcggactgta
     1201 acccgacccg gggggtatca tccggcgtaa aaaacgatcg ctccggacac cgcgccccct
     1261 ctcacgcaga gaaaaaattc cccttcagaa gttataaaca tgtttataac ctactgatgg
     1321 atttttaccg tttttttctt tttattcata acgttgtggg aggtaaatgt ttataccttt
     1381 ttttcgcaaa atgtttacag ggcagcggta aacattttcc ctgccgcctc cattcttacc
     1441 gggaggattg ctgacgtttt tctgtttttc cccggaggaa agaatgctga cgacgcaaaa
     1501 aagaaaattt gcgctggcgc tcatgtcggg caaaaacaga acggctgctg ctattgaggc
     1561 tggttactcg gaaaagtccg ccagatgtaa aggctcacaa ctggcaaaag atccggaagt
     1621 gctcgcgttt atagcccgta agcagggcga gacggtggag gtggatgagg ttcctgttta
     1681 ccggcagaaa aagacgcagg cgtgccaccg ggaagttcct ccggtggaaa agaaaaacca
     1741 ggtttcagtg ccaccggatg acgggcaaag tttctctcca ctatccggta ttgattacat
     1801 ggaggacggc cttcctgatc ctgtgaaagc catgggtcgg atcctggtgg aaaacatcag
     1861 cattgatccg aaactggctc tggatgcagc ctggcgactg gcgcagttca cgcaccacaa
     1921 aaaaggggat gccggtaaaa aatcggcaaa aggtgacgcg gcgaaaaaag cggctaaccg
     1981 ttttgcggtg ccaccaccac cccgcctggt ggtgaataat gataatgagg gcaacggatg
     2041 atacctgtgt ggagcacggc ctgcccggac tgggcagagc gtctgaaaaa ggggctgtcg
     2101 attattccgg ctccgattta cccggaccag gccgcacatg ccctggcgat ttttaaacaa
     2161 ctgcggattg tggatgcacc gggcagcccg acgttcgggg agtcctgtgc accgtgggtg
     2221 tttgatctgg tggctgccct gtttggctcc tacgatgcgc agaccggtgt acgccatata
     2281 aaggaagtgt tcattctgat acccaaaaaa aacagcaagt ccacgctggc tgccgggatc
     2341 atgatgacgg cgctgttact gaactggcgg caggcggcgg gctacaccat tctggccccg
     2401 accgtggagg tggcggctaa cgccttcaac cctgccaggg atatggtacg acgggacgat
     2461 gatctggatg acctctgtca ggtgcagaca catatccgga ccatcaccca tcgggtgacg
     2521 gacaccaccc ttaaggtggt ggctgccgat ccgaatacgg tgtccggtat caagtccgtg
     2581 gggacgctga ttgatgaact gtggttattt ggcaagcagt acaaagcgga ggacatgtta
     2641 cgtgaagcca tcggcggcct tgcctcccgc ccggaagggt ttgtggtgta cacaaccacc
     2701 cagtcgaatg agccgcccgc cggggtgttc agacagaaac tgcagtacgc ccgggatgtg
     2761 cgcgacggca aaattaatga tccgcacttt ctgccggtga tatttgagca tccgccggag
     2821 atggtggagc gcggtgagca cctgctgatg gaaaacctcg ccatggttaa cccgaatctc
     2881 ggttattcgg tggatgaggc ttttctgtac cgggagtacc gtaaagcccg ggaggctggt
     2941 gaggaggcat ttcgtggctt catgtcaaaa cacgccaatg tggaaattgg tcttgccctg
     3001 cgttctgacc gctgggcggg tgcggatttc tgggagcagc agggcaggcg cgtcagcctg
     3061 gacgatatcc tgcagcgcgc tgatgtggtg acggtgggga ttgacggcgg gggcctggat
     3121 gatctgctgg gaatgtacgt gattggtcgt gacagggaaa cccgcgaatg gctgggctgg
     3181 ggccatgcct gggcgcatga aaccgcggtg gtcagacgga agagtgaggc atcccggttt
     3241 caggattttg tggccagtgg agacatgacg attgtccgtc gggtcgggga tgacacggcg
     3301 gaagtggcgg agtatgtgcg tcgtattcat gaggctgagt tactggacca tatcggtatt
     3361 gacccgtcag gtgtggggca gattctggat tcactggcag aagccgggat ccccgacgga
     3421 attgttgtgg ggataagcca gagctggaag ctgggcgggg ccat
//
LOCUS       DAZKFU020000353         3416 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZKFU020000353 DAZKFU020000000
VERSION     DAZKFU020000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.355"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            148..1812
                     /gene="tsr"
                     /locus_tag="ACHOQQ_RS23020"
                     /old_locus_tag="pgaptmp_004604"
     CDS             148..1812
                     /gene="tsr"
                     /locus_tag="ACHOQQ_RS23020"
                     /old_locus_tag="pgaptmp_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGU4472756.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(1861..3222)
                     /locus_tag="ACHOQQ_RS23025"
                     /old_locus_tag="pgaptmp_004605"
     CDS             complement(1861..3222)
                     /locus_tag="ACHOQQ_RS23025"
                     /old_locus_tag="pgaptmp_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4472757.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
BASE COUNT          913 a          819 c          931 g          753 t
ORIGIN      
        1 aaaagaaaat gtatcaataa gtggttattg tatttttgaa caaaaattaa aaaagtgagg
       61 taaaattaac cagtttcaag tcgctaccta taaagttttc tcatttcagg ccgaaaatct
      121 tgcatcggtc cacaggaaag agaaaccatg ttaaaacgta tcaagatcgt gaccagctta
      181 ctgctggttt tggccgtttt tggcctttta caactgacat caggcggtct gttctttaat
      241 gccttaaaga atgacaaaga aaatttcact gttttacaaa ccattcgcca gcagcaatcc
      301 acgctgaatg gcagctgggt cgcgttgttg cagacgcgta acaccctcaa ccgtgcgggt
      361 atccgctaca tgatggatca gaataatatt ggtagcggtt caaccgttgc tgagctgatg
      421 cagagtgcca gtatttcgct gaaacaggcg gaaaaaaact gggcagatta cgaagcgttg
      481 ccgcgtgacc cgcgtcagag caccgccgca gcggcagaga tcaaacgtaa ttacgatatt
      541 tatcacaatg cgctggcgga gctgatccaa ctgttaggtg caggcaaaat caacgagttc
      601 tttgatcagc cgacccaggg atatcaggac ggtttcgaga agcagtatgt ggcttacatg
      661 gagcaaaacg atcggctcta tgatatcgcc gtcagcgata acaatgcctc ctacagccag
      721 gcgatgtgga ttctggtggg cgtgatgatc gtcgtactgg cggtcatctt cgccgtctgg
      781 ttcggtatta aagcctcgct ggtagcgcca atgaatcgtc tgattgacag cattcgtcat
      841 attgcaggcg gcgatctggt gaaaccgatt gaggtggatg gctctaatga gatggggcaa
      901 ctggcagaaa gtttgcgcca tatgcaggga gagctgatgc gtaccgtcgg tgatgtgcgc
      961 aacggggcca atgccatcta tagcggtgcc agcgaaatcg ccaccggtaa taacgatctc
     1021 tcttcgcgca ccgagcaaca ggccgcttcg ctggaagaga cggcagccag catggagcaa
     1081 ctgaccgcaa cggtgaaaca gaacgccgag aatgcgcgcc aggccagcca cctggcgtta
     1141 agtgcttctg aaacggcgca acgcggcggc aaagtggtag ataacgtggt gcagactatg
     1201 cgcgatatct ccaccagttc gcagaaaatc gccgatatta tcagcgtaat tgacggcatt
     1261 gctttccaga ccaatattct ggctttgaac gcggcggttg aggctgcgcg tgcgggtgag
     1321 caagggcgcg gttttgcggt ggttgcggga gaagtgcgta atctggccca gcgtagcgcc
     1381 caggcggctc gtgaaattaa aagcctgatt gaagactcgg tggggaaagt ggatgttggc
     1441 tctacgctgg tcgaaagcgc cggggaaaca atggcggaga ttgtcagtgc tgtgacccgc
     1501 gtgacggaca ttatgggcga aatagcttct gcttctgatg agcagagccg tggtatcgat
     1561 caggttggct tagcggttgc tgagatggac cgggtaactc aacagaacgc cgcgctggtg
     1621 gaagaatctg ccgctgccgc cgccgcgctg gaagagcagg ccagtcgcct gaccgaagct
     1681 gtggcagtgt tccggattca gcaacagcag caacagcagc gtgaaacatc ggctgtggta
     1741 aaaaccgtga cgccagctac gccgcgtaaa atggcagtgg cagatagcgg ggagaactgg
     1801 gaaacgtttt aatcgccata aaaatgcccg ataagcaaaa tgttatcggg catagggagt
     1861 ttaatcttta cgcgggtcgt tgatcggctg acgaaccagg aagatgtacg ccagtgctcc
     1921 cgctgcggtg acgcaaccgc aaatgattag tgccagacgg aatgagtggg ttgtatcaac
     1981 aataaaacca gtaatgatcg gcgcgaaaga ggcgcagatg aagctggcaa agttctggat
     2041 actgcccacc gacgcagtca tgcgagaagc aactgcgacg tggatcaagc cccagcagga
     2101 tgttccggca aagtgaatac agaacagtgc cataccaatc agcagaaccg ctgtcatgga
     2161 tgttgtcgct tgcggtacta tcagcgtaaa ggcggcagaa cagaacatcc cggcaataat
     2221 gcagatctta cggcttttaa tcggagccat tccccctttg accagccagt cagtaacgta
     2281 accgttgacc agcatcccgg cagccccaaa caggaaaggg atagccgcca tcaaccctgt
     2341 actttttaaa tccaggttat aggctgtttg caggtaacca ggaagccaag ccagatacag
     2401 ccacgcagtg tagttgatgc cactgaatcc gagcatcatt ccccacattg tgcggttacg
     2461 gaacaggctg cgccattcgg caaaactgag cggatctcgg cgggcattga cgctacctgc
     2521 attgagataa gcttgttcaa cggcagtcag ttctacgtgc tcgcggttgc gatagagcat
     2581 ataccagccg atggcgagaa aaatccccag tacaccaatg gtaataaaca tcccgcgcca
     2641 gcccatcacc agcatcatcg ccgccagaat cggtgggctt acggcaacgc caatggtaga
     2701 agctgcgttg aagaagccca tcgggcgtcc gcgctctttg atgttgaacc agtcgttaat
     2761 gacttttaca ccgcatgggt tcatcggcgc ttcaccaatc cccataccga tacgcaccaa
     2821 cacgaactgc gtaaagctgt gcaccatgcc agacatggcc tggaacagtg accagaagaa
     2881 catccccagt cccagcatca ggcgtgggcc tttacgatcc aatagtgggc cgcaaggaag
     2941 ttgcgcaatg ccgtaagcga gtgaaaacac ggagagcaaa gcgccgattt cggtggcact
     3001 taatcccaat tcttcacgaa tcgttaaatt tgctaccgac agcgaactgc ggtcgagata
     3061 attgattacc gccgcaaaaa ataataacaa catggcggtg gtttgaatgc gtttaattcg
     3121 ggtactgcgt tgaactaatc catctggtgg cacgggaata tctgccgacg aagatggagt
     3181 aaatgaagaa cgcggatcga gggtgatatt ttctttttcc acgccggact cctgtattta
     3241 taattttaat tgtttacgta attgtgcatt acgcaaggtt gacattaacg gcaggttaaa
     3301 gttttaggac tttactaccg ctcacaaaaa catttttagt cgactgattg tgagcatagg
     3361 taagcgggaa tttcccggta tttatctttt gataaaaccg tgcacccgct aatagt
//
LOCUS       DAZKFU020000354         3409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZKFU020000354 DAZKFU020000000
VERSION     DAZKFU020000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.356"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..1742
                     /gene="aslA"
                     /locus_tag="ACHOQQ_RS23030"
                     /old_locus_tag="pgaptmp_004606"
     CDS             87..1742
                     /gene="aslA"
                     /locus_tag="ACHOQQ_RS23030"
                     /old_locus_tag="pgaptmp_004606"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HGU4472758.1"
                     /translation="MEFSFSPKRLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     DYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1910..3145)
                     /locus_tag="ACHOQQ_RS23035"
                     /old_locus_tag="pgaptmp_004607"
     CDS             complement(1910..3145)
                     /locus_tag="ACHOQQ_RS23035"
                     /old_locus_tag="pgaptmp_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4472759.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRYYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMVDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(3292..3368)
                     /locus_tag="ACHOQQ_RS23040"
                     /old_locus_tag="pgaptmp_004608"
     tRNA            complement(3292..3368)
                     /locus_tag="ACHOQQ_RS23040"
                     /old_locus_tag="pgaptmp_004608"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3332..3334),aa:Pro,seq:tgg)
BASE COUNT          865 a          919 c          842 g          783 t
ORIGIN      
        1 gtaatcatcc tgtcagggag agggatctca attatcaatg cttaattacg tcatcatttt
       61 gatgacatga aaccggagca ttttgcatgg aattttcgtt ttcacccaaa cgtcttgttg
      121 ttgctgtcgc cgccgctctt cctctcatgg ccagcgcagc agataccccg tcaactgcca
      181 ccgcacgcaa aggctttgcc ggatacgatc acccaaacca gtatctggtt aaaccggcga
      241 ccactattgc cgacaacatg atgccagtaa tgcagcatcc ggcgcaggat aaagaaaccc
      301 agcagaagct ggcagaactt gagaaaaaaa ccggtaagaa accgaatgtg gttgttttct
      361 tgctggacga tgtgggctgg atggacgtcg gttttaacgg tggcggcgtg gcggtgggta
      421 accctacacc agatatcgac gccgttgcca gccaggggct gattttaact tcggcgtatt
      481 ctcaaccaag ctcttcccca acccgcgcca ccattctcac cggacaatac tccatccacc
      541 acggcattct gatgccgcca atgtacgggc aaccgggcgg gctgcaaggg ttaaccacgc
      601 tgccacagtt gctgcacgat cagggctacg tcactcaggc catcgggaaa tggcatatgg
      661 gggaaaacaa agagtcgcag ccgcagaacg ttggctttga tgatttccgt ggctttaact
      721 cggtgtctga tatgtacacc gaatggcgcg acgttcacgt caatccggaa gtggccctga
      781 gtccggaccg ttctgaatac atcaagcaat taccgttcag caaagatgac gttcatgcgg
      841 tgcgcggcgg cgaacaacag gccattgccg atattacgcc gaaatatatg gaagatctgg
      901 atcaacgctg gatggactat ggcgttaagt tcctcgacaa gatggcgaag agtgataagc
      961 ctttcttcct ctactacggc actcgtggct gccacttcga taactaccca aatgcgaaat
     1021 atgcaggtag ctctccggca cgcacctcgt atggcgactg catggtggaa atgaacgatg
     1081 tgttcgctaa tctgtataaa gcactggaga aaaacggtca gcttgataac acgctgattg
     1141 tctttacctc cgataacgga ccggaagccg aagtaccgcc gcacggacgc acaccgttcc
     1201 gtggtgcgaa aggatctacc tgggaaggcg gcgttcgcgt accgactttc gtgtactgga
     1261 aaggcatgat ccaaccgcgt aaatctgacg gtattgtcga tctggcagat ctcttcccta
     1321 ccgcgctgga tctggcaggg catcctggcg cgaaagtggc gaatttagtg ccgaaaacca
     1381 cctttatcga tggtgtggac cagacatctt tcttcctggg aacaaatggt cagtctaacc
     1441 gtaaggccga gcactacttc ctcaacggta aactcgctgc tgtgcgtatg gatgaattca
     1501 agtatcacgt cctgattcag caaccttacg cttataccca gagcggatat cagggtggat
     1561 tcaccggcac agtaatgcaa acggcgggat cgtcggtgtt taacctgtac accgatccgc
     1621 aggaaagcga ctccattggc gtgcgccata ttccgatggg tgtaccgcta cagaccgaaa
     1681 tgcacgcgta tatggagatc ctgaaaaagt atccaccacg cgcgcagatt aaatctgatt
     1741 aagccggcgc tatcgctgag atctgccttt gccggatgcg atgctgacgc atcttatcca
     1801 gcctacagaa cgctgcaatt tattgaattt gcacgatcat gtaggccgga taaggcgttt
     1861 acgccgcatc cggcaatcaa ccgcaggcgg ccacaaaatg gccgcttttt tacttactca
     1921 ccaccagcaa atgcgcatgc ataatgtcgc tggccggacg accgtgcgcc agcaaatcag
     1981 ccatagcttt aagatatggc ggtagatggc ggaaataacg ctgatacccg gcacacaaat
     2041 aattcagccc cggtttgccg ctggcatcga gcatgaagcg gtgtttcgga cagcctcccc
     2101 aacatgcttt taacacgtta caactgcgac actgcgccgg taactgctta aatttatctt
     2161 caccaaaagc ctgctgctgc ggagaatcta ccatttctgc aattgtttgc tggtgcatat
     2221 tccccagtcg atattgcgga taaacatagt gatcgcaggc gtaaacgtcg ccgttgtgct
     2281 caacaatcac cgagcgccca caggttggct gatgatggca aaccgcaccc ggcgcaccga
     2341 caaaattggc aaacgcccat tcgatattca tcacaaaaat cttgccgaca tcacgtttga
     2401 tccagtggtc gaaaatcgcc accagaaatt caccaaattc atcggggcgc accgaccatt
     2461 ccgttagctc accctgaata tcaccaggcg catgcaattt cagtccttcg cgggcagccg
     2521 tttcatcagc caagcgctcg accaccggaa taaactggat gaattccact cccgcatcgc
     2581 acaaaaaatc atatacctgc aacggttgct gcgcgctggt gcgattaacg cagaccagca
     2641 cgttatagtc gacatgatgt ttttgcagca gcgtcagggc acgcatcacc agcttatgcg
     2701 ttggtctgcc acctttggtc acgcgatatt gattgtggat ctcagccgga ccatccagcg
     2761 ataacccaac aagaaaatga ttttctgcca gaaatgcaca ccattcgtca tcgagcagca
     2821 cgccgttagt ctggaagctg ttacttatct tcctgccagc accatatttc gcctgtagcg
     2881 ctacggcacg gcggtaaaac tccagcccga gtagcgttgg ttcgccgccc tgccaggtaa
     2941 aagccacttc gttttgcggt tcgctggcag cgatatagta acggacatac gcttccagcg
     3001 tgtcatcgtc catatgcgtg actggttttt cgcggtaaag ggattgtttt tcgagataaa
     3061 aacagtagtc acagttcaga ttacaatcgg aaccgctcgg tttcgccatc acatgaaaag
     3121 cacgcgttgg aacctgttgc agcatggtcg ctccttgcct gaagtcatcc ttcaatacgt
     3181 taagggcgaa atcataccgt ggggcaatga gttattacgg gcgtttattg cgaaggaagg
     3241 gtgatagaat gcacagaaaa caaaaaaccc cgccgaagcg gggttcaaaa ttggtcggcg
     3301 agagaggatt cgaacctccg acccactggt cccaaaccag ttgcgctacc aagctgcgct
     3361 actcgccgaa atactgcttt ttgaattttt agttcaattc tttaaagtc
//
LOCUS       DAZKFU020000355         3370 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZKFU020000355 DAZKFU020000000
VERSION     DAZKFU020000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.357"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2050
                     /locus_tag="ACHOQQ_RS23045"
                     /old_locus_tag="pgaptmp_004609"
     CDS             <1..2050
                     /locus_tag="ACHOQQ_RS23045"
                     /old_locus_tag="pgaptmp_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4472760.1"
                     /translation="KSRKSVKSVEPDIADRANNWLKSYGLDYKLEQAPLNNEIDNALD
                     AYFSKNGGTGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDGTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDARPTLDDFKKTVGDYLAWEVSQLLKKQGENNFAGK
                     "
     gene            2119..2616
                     /locus_tag="ACHOQQ_RS23050"
                     /old_locus_tag="pgaptmp_004610"
     CDS             2119..2616
                     /locus_tag="ACHOQQ_RS23050"
                     /old_locus_tag="pgaptmp_004610"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4472761.1"
                     /translation="MSTGSLISSGRLKPGKIKRVSAKSDQNGIIGEFDTEFMDDARHY
                     ENFISVNFFGDAFYHPYCASVEMKVHVLTLKSSKSDFNAKRGLYVASMINKALKGRFN
                     YGQQLSSSKLRDGEYFISLPVKNNEIDWFFMDNYIEELEAAHIEELEAYLLVSGLADS
                     RTDKL"
     gene            2669..3190
                     /locus_tag="ACHOQQ_RS23055"
                     /old_locus_tag="pgaptmp_004611"
     CDS             2669..3190
                     /locus_tag="ACHOQQ_RS23055"
                     /old_locus_tag="pgaptmp_004611"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4472762.1"
                     /translation="MWGTYNLKKLFGPAIRGKRLKNADRIPGSLPFVTAGEGNEGISA
                     FISNDVVVFPENTITIDMFGSAKYRNYEYGADDHVAVVHTEFFDRNAVIFITASIHKS
                     SCTGKFNYGRNFYAKDADELMVSLPSKDNNPDYSFMETLILAVHKLVIKDVVQYTDKK
                     LVAYRQITSEKVV"
BASE COUNT         1041 a          625 c          798 g          906 t
ORIGIN      
        1 taaatcaaga aagtctgtaa aatctgttga accagacatt gccgaccggg ctaacaactg
       61 gctcaaatct tatggacttg attacaaact ggagcaggca ccacttaaca acgagatcga
      121 taacgcactg gatgcttatt tttcgaaaaa tggcggcaca ggcggtaatc gtcccgacgt
      181 aaaactcctt gttcaggaca aatacggcaa gcagtatccc gttctcatcg agtataaagg
      241 ctacaaggac aggcttatca gacttggcag caacggtatt attgagaaca aagatgccag
      301 aaaagaatgg aattttaaaa atattaatgg atacgcggtg aatggcgcag tccattatgc
      361 caacgcactt cttcagttca ccaattaccc tgatattatt gctattggta tgaccggctg
      421 gcgcgatgat ggtacaggcg aactgcatca tgaaatcggc gtgtggtacg tatcaaagaa
      481 taatttagga gccgggcaga aggttggtga attcaccgac ttatcgttcc ttgctgacaa
      541 aaatgttgat ggatttctca acaagataaa gctgcttaat cttcctcccg aagaactgga
      601 aaaaatcaag gccagcaaag aggaagagat agacacgcga ctttcccgtc tgaataacga
      661 tatttaccag aatgaaaagg ggcttggtga aagcgatcgt gtttaccttg ttgtggctac
      721 cgttattgca acgttgggga ttccgggaaa gttagcaccg ctggataaaa aagaactgac
      781 ctcttcaacg gaggaggact tgcgtgatgg tgatattatt ttcaggaaaa taaggaattt
      841 tttaaggctg aaagctgttc ctgaaacaaa aagagaaatg attttgcgct cgttgcaaaa
      901 tacgctgtgg actgaaaaca ttaacaaacc agtcaacggt gaaagccagt taaagcgcgt
      961 gtttgttaaa gttgttgatg atcttgggga gtattacaaa atcggactga ccactgattt
     1021 tacgggcaaa ctgtttaatg agatgtatcg ctggcttgga tttacgcagg acaaactcaa
     1081 tgacgtcgtt ctgacaccac cttatgttgc cacattgctg gcacgactcg ccagagtaaa
     1141 caaagattcc tatgtgtggg attttgctac aggttctgcg ggattgcttg tggctgcaat
     1201 gaatgaaatg ctcattgacg ccagagaaaa tattcactca ccgaatgaac tacaacttaa
     1261 ggaagcgcaa atcaaggctg aacagcttct tggccttgag gtgttatcca gtatttatat
     1321 gctggccatc ctgaatatga ttttgatggg ggatggtagc tcgaatatcc tcaataaaga
     1381 ctcgctagcc gattttgacg gcaagtatgg atttgggaaa acaggagaaa aattccctgc
     1441 ggacgcattt attctcaatc ctccgtattc agctaaaggt aacggcatga ttttcgtgca
     1501 gaaggcgctg tcgatgatgg ataaaggcta tgctgcagtg attatccaga gttcagccgg
     1561 cacaggaaag gctacggagt acaacaaaaa aatactcaag gaaaatacct tgctggcaag
     1621 catcaaaatg cctgcggatc tgtttattgg taaatcaagc gttcagacgt acatttacgt
     1681 ttttcaggta aaaattccgc ataacgcgaa acaggctgtt aagtttatcg acttttccaa
     1741 tgacggctat gctcgctcta atcgcaaaaa ggcaagaaac aatcttgttg atgccgatcg
     1801 tgcaaaagag cgttatcagg aggttgtgga tctggttcac ttcgggaaag gttgccttaa
     1861 tatctttacg gaggatgaat attttgaggg aacaatcgat cctgatagtg gtgaggactg
     1921 gaatcagacc aggccagtag atgctaggcc aacgctggat gatttcaaaa agacggtagg
     1981 tgattacctt gcatgggagg tctcgcaatt actgaaaaaa caaggtgaaa acaattttgc
     2041 gggaaagtaa attccccgct cagcgataaa ttaagaagcg ttgagtgggg agagttcagg
     2101 attggcgatt tgttctatgt gagcacaggc tcgcttatct cctcaggaag attaaaacca
     2161 ggcaaaatta aacgtgtttc agcaaagagc gatcaaaacg gtatcatcgg agagtttgat
     2221 accgagttta tggatgatgc aaggcattat gaaaatttta taagcgttaa tttttttgga
     2281 gatgcttttt atcatcctta ttgtgccagc gtagaaatga aggtgcatgt tcttacatta
     2341 aaatcaagta agtccgactt taatgcaaag cgcggactgt atgttgccag tatgattaat
     2401 aaagctttaa aggggcgttt taattatggt caacagttat cgagttccaa actcagagat
     2461 ggagaatatt tcatctcatt accagtgaaa aataatgaaa ttgactggtt ttttatggat
     2521 aattatatag aagagctgga agcggcccat atagaagagc tggaagctta cttgctggtt
     2581 agcggactcg cggactcgcg gactgataag ctatgagctt acaccgcagg aatataacgc
     2641 gattgagcta ttaaatagca ataatcttat gtggggcact tataacctca aaaagttatt
     2701 tggccccgca ataagaggta agcgtctgaa aaacgcagat cgtattcccg gatccttgcc
     2761 ttttgttact gcgggtgaag gcaatgaagg tatttctgca tttatcagta atgatgttgt
     2821 tgtattccct gaaaatacca ttacgataga tatgtttggc tccgcaaaat acagaaatta
     2881 tgagtatggt gcagatgacc atgtggcagt tgttcataca gaattctttg acagaaatgc
     2941 cgttattttc ataacagcat caattcataa gtcttcttgt accggaaagt tcaactacgg
     3001 gagaaatttc tatgcaaaag atgccgacga gttaatggta tctttgccgt caaaagataa
     3061 taaccctgat tattctttca tggagacgct aattttagca gtacataaac tggtcatcaa
     3121 agatgttgtc cagtacactg ataaaaagct ggtagcgtac aggcagatta catctgagaa
     3181 ggtcgtttag tgcaaatccc cacctgacag ggtggggaat ggtcaggtta tcgtgccctg
     3241 atgcagaaaa acagtttaca atcgtaaaaa tcatcatatc atgacaaaag tggttacatt
     3301 gccacgcagc cgaacccgcc gatgcgcggg ttttttgtat ccggaatcct gtgagttata
     3361 cggaaagtac
//
LOCUS       DAZKFU020000356         3366 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZKFU020000356 DAZKFU020000000
VERSION     DAZKFU020000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.358"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            92..1243
                     /gene="yiaY"
                     /locus_tag="ACHOQQ_RS23060"
                     /old_locus_tag="pgaptmp_004612"
     CDS             92..1243
                     /gene="yiaY"
                     /locus_tag="ACHOQQ_RS23060"
                     /old_locus_tag="pgaptmp_004612"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGU4472763.1"
                     /translation="MAASTFFIPSVNVIGADSLADAMNMMAEYGFRRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSIIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1408..2946
                     /gene="aldB"
                     /locus_tag="ACHOQQ_RS23065"
                     /old_locus_tag="pgaptmp_004613"
     CDS             1408..2946
                     /gene="aldB"
                     /locus_tag="ACHOQQ_RS23065"
                     /old_locus_tag="pgaptmp_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGU4472764.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAYTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT          836 a          823 c          898 g          809 t
ORIGIN      
        1 ttaattttgc taaacaataa tactgatcat attttcgaat gaaaatcctc agtaagctgc
       61 ccgccctttt ttacactttc aggagtgtgt tatggcagct tcaacctttt ttattccttc
      121 tgtgaatgtc atcggcgctg attcattggc tgatgcaatg aatatgatgg cagaatatgg
      181 gtttcgccgt acattgattg tcactgacag tatgttaacg aaattaggta tggcgggcga
      241 tgtgcaaaaa gcactggaag aacgcaatat ttttagcatt atttatgatg gtacccaacc
      301 taaccccacc acggaaaacg tcgccgcagg tttgaaatta cttaaagaaa ataattgcga
      361 tagcgtgatc tccttaggcg gtggttctcc gcacgactgc gcaaaaggta ttgcgctggt
      421 ggcagccaat ggcggcgata ttcgcgatta cgaaggcgtt gaccgctctg caaaaccgca
      481 gctgccgatg atcgccatca ataccacggc gggtacggcc tctgaaatga cccgtttctg
      541 catcatcact gacgaagcgc gtcatatcaa aatggcgatt gttgataaac atgtcactcc
      601 gctgctttct gtcaatgact cctcgctgat gatcggtatg ccgaagtcac tgaccgccgc
      661 aacgggtatg gatgccttaa cgcacgctat cgaagcgtat gtttctattg ccgccacgcc
      721 gatcactgac gcttgtgcac tgaaagccgt gaccatgatt gccgaaaacc tgccgttagc
      781 cgttgaagat ggcagtaatg cgaaagcgcg tgaagcaatg gcttatgccc agttccttgc
      841 cggtatggcg ttcaataatg cttctctggg ttatgttcat gcgatggcgc accagctggg
      901 cggtttctac aacctgccac acggtgtatg taacgccgtt ctgctgccgc atgttcaggt
      961 attcaacagc aaagtcgccg ctgcacgtct gcgtgactgc gccgctgcaa tgggcgtgaa
     1021 tgtgacaggt aaaaacgacg cggaaggtgc tgaagcctgc attaacgcca tccgtgaact
     1081 ggcgaagaaa gtggatatcc cggcaggcct acgcgacctg aacgtgaaag aagaagattt
     1141 cgcggtactg gcgactaatg ccctgaaaga tgcctgtggc tttactaacc cgatccaggc
     1201 aactcacgaa gaaattgtgg cgatttatcg cgccgcaatg taatcatcat ttccacaacg
     1261 gctggcaaat tgtcagccat tttttcaact atctctgtaa tccttgcccg taaattcgtg
     1321 atagctgtcg taaagctgtt accgactggc gaagatttcg ccagtcacgt ctacccttgt
     1381 tatacctcac accgcaagga gacgatcatg accaataatc ccccttcagc acagattaag
     1441 cccggcgagt atggtttccc cctcaagtta aaaaaccgct atgacaactt tattggcggc
     1501 gaatgggtag cccctgccga tggtgagtat taccagaacc tgacgccggt gaccgggcag
     1561 ctgctgtgcg aagtggcgtc ttcgggcaaa cgagacatcg atctggcgct ggatgccgcg
     1621 cataaagtga aagataaatg ggcatatacc tcggtgcagg atcgcgcggc aattctgttt
     1681 aagattgccg atcgaatgga acaaaacctc gagctgttag cgacagctga aacctgggat
     1741 aacggcaaac ccattcgcga aaccagtgct gccgacgtgc cgctggcgat tgaccatttc
     1801 cgctatttcg cctcgtgtat tcgggcgcag gaaggtggga tcagtgaagt tgatagcgaa
     1861 accgtggcct atcatttcca tgaaccgtta ggcgtggtgg ggcagattat cccgtggaac
     1921 ttcccgctgc tgatggcgag ctggaaaatg gctcccgcgc tggcggcggg caactgtgtg
     1981 gtgctgaaac ccgcacgtct taccccgctt tctgtactgc tgctaatgga aattgtcggt
     2041 gatttactgc cgccgggcgt ggtgaacgtg gtcaatggcg caggtgggga aattggcgaa
     2101 tatctggcga cctcgaaacg catcgccaaa gtggcgttta ccggctcaac ggaagtgggc
     2161 caacaaatta tgcaatacgc aacgcaaaac attattccgg tgacgctgga gctgggcggt
     2221 aagtcgccaa atatcttctt tgctgatgtg atggatgaag aagatgcctt tttcgataaa
     2281 gcgctggaag gctttgcact gtttgccttt aaccagggcg aagtttgcac ctgtccgagt
     2341 cgtgccttag tgcaggagtc aatctacgaa cgctttatgg aacgcgccat ccgccgtgtc
     2401 gaaagcattc gcagcggtaa cccactcgac agcgtgacgc aaatgggggc gcaggtttct
     2461 cacgggcaac tggaaaccat cctcaactac attgatatcg gtaaaaaaga gggcgcagac
     2521 gtgctcaccg gcgggcggcg caagctgctg gaaggcgaac tgaaagacgg ctactacctt
     2581 gaaccgacga ttctgtttgg tcagaacaat atgcgggtgt tccaggaaga gatttttggc
     2641 ccggtgctgg cggtgaccac cttcaaaacg atggaagaag cgctggagct ggcgaacgat
     2701 acgcaatatg gcctgggcgc gggcgtctgg agccgcaacg gtaatcttgc ctataagatg
     2761 gggcgcggca tacaggcagg gcgcgtgtgg accaactgtt atcacgctta cccggcacat
     2821 gcggcgtttg gtggctacaa acaatcaggc attggacgcg aaacccacaa gatgatgctt
     2881 gagcattacc agcaaaccaa gtgcctgctg gtgagctact cggataaacc gttggggctg
     2941 ttctgatgta agaagctggt cgcatcgggt attaattgcc tgatgcgacg cttacgcgtc
     3001 ttatcatgcc tacggggacc tgaccgtagg ccggataagg cgtacacgcc gcatccggca
     3061 taaacaaagc gcacgttgtt acaatttacg ttttaaacct acgtgcgcaa taacccagac
     3121 tacgccaaca gaagtgcgtt tctggggcgt gttttgccag gagtttaatc tcgcgtttcg
     3181 atggtgagcg ggcaaaccag accgtaaatc atcattggta ttccgaggac aatcagcaat
     3241 aaacggtaga ttattgaagg tttaatacgc ttaatctgca ttgtggatgt tttacggttt
     3301 ttaatcataa attttgccaa tagttccatt atttcaatat gataagcctc gacctgttaa
     3361 aaagcc
//
LOCUS       DAZKFU020000357         3359 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZKFU020000357 DAZKFU020000000
VERSION     DAZKFU020000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.359"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..254
                     /locus_tag="ACHOQQ_RS23070"
                     /old_locus_tag="pgaptmp_004614"
     CDS             48..254
                     /locus_tag="ACHOQQ_RS23070"
                     /old_locus_tag="pgaptmp_004614"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4472765.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRVFVARIAREHDIND
                     NLLFKWLRLWLIKGKRKPTAVCSH"
     gene            370..732
                     /locus_tag="ACHOQQ_RS23075"
                     /old_locus_tag="pgaptmp_004615"
     CDS             370..732
                     /locus_tag="ACHOQQ_RS23075"
                     /old_locus_tag="pgaptmp_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4472766.1"
                     /translation="MVKLALQPGASVARIAREHDINDNLLFKWLRLWQNEGRVSRRLP
                     VTTSSDTGVELLPVEITPDEQKEPVVAIAPSLSTTTQTRVSASSCKVEFRHGNMTLEN
                     PSPELLTVLIRELTGRGR"
     gene            729..1079
                     /gene="tnpB"
                     /locus_tag="ACHOQQ_RS23080"
                     /old_locus_tag="pgaptmp_004616"
     CDS             729..1079
                     /gene="tnpB"
                     /locus_tag="ACHOQQ_RS23080"
                     /old_locus_tag="pgaptmp_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4472767.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRRNSLTML"
     gene            1110..2534
                     /locus_tag="ACHOQQ_RS23085"
                     /old_locus_tag="pgaptmp_004617"
                     /pseudo
     CDS             1110..2534
                     /locus_tag="ACHOQQ_RS23085"
                     /old_locus_tag="pgaptmp_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
     gene            complement(2787..>3359)
                     /locus_tag="ACHOQQ_RS23090"
                     /old_locus_tag="pgaptmp_004618"
     CDS             complement(2787..>3359)
                     /locus_tag="ACHOQQ_RS23090"
                     /old_locus_tag="pgaptmp_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HGU4472768.1"
                     /translation="IAFQHYCQSLEQIEDRILQLDQEISRLLPEWSLCNLVCQLQALK
                     GVGQLIAITLVAELGDFSRFSNPKQLMAFLGLVPGEYSSGNSIRPRGITKVGNSELRR
                     LLYEAAWSYRTPAKVGAWLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQV
                     AITAVARELTGFMWDIALAAQSSFSQQKQN"
BASE COUNT          871 a          826 c          865 g          797 t
ORIGIN      
        1 tattggtaac gtaggtgccc accttttcaa actagtggat gcctgttatg gaacaaaaaa
       61 cattatccgc agaaccccgc aggtcatttt caaatgagtt taaacttcaa atggttaaac
      121 tggctttaca accaagggtt tttgttgccc gtattgcccg ggagcatgat atcaatgata
      181 atcttctgtt caaatggctc aggctctggc tgatcaaagg taagcgtaaa ccgactgccg
      241 tatgtagcca ttaagccagt attggtaacg taggtgccca ccttttcaaa ctagtggacg
      301 cctgttatgg agcaaaaaac attatccgca gaaccccgca gatcattttc aaataatttt
      361 aaacttcaaa tggttaaact ggctttgcaa ccaggagcct ctgttgcccg tatcgcccgg
      421 gaacacgata tcaatgataa cctcctgttc aaatggctca ggctctggca gaacgaaggg
      481 cgtgtttcgc ggcgtcttcc ggtaacgacc tcttctgaca ctggcgttga attattacct
      541 gtagaaataa cgccggatga gcagaaagaa cctgtggtgg ccattgcgcc atctttatcc
      601 actaccactc agaccagagt cagtgccagc tcctgcaagg tggaattccg tcacggtaac
      661 atgacgctgg aaaatccatc gccagagctg ctcacggtgt tgatccgtga actgaccggg
      721 aggggaagat gatctcactc ccatcaggta cccgtatctg gctcgttgcc ggcgttaccg
      781 atatgcgtaa atccttcaac ggactgggag aacaggtaca acatgtgctg aatgataatc
      841 ccttctccgg tcacctgttt atcttccgtg gccgacgggg tgacaccgtt aaaattcttt
      901 gggctgatgc tgatggtctg tgcctgttca ccaaacgcct ggaggaaggc cagtttatct
      961 ggcctgcggt acgtgacggc aaggtatcca ttacccgctc gcaactggca atgctcctcg
     1021 ataagctgga ctggcgtcag ccaaaaacat cccgccgtaa ctcactgaca atgttgtaaa
     1081 aaactcctga ccgcattata aaaacggtca tgagtcagaa atacctcatt cgcatcgcag
     1141 agctggaaag gttgctctct gagcaggctg aagccctccg tcagaaagac cagcaactga
     1201 gtctggttga agagacggaa gccttcctgc gctctgcact gacacgtgcc gaagaaaaga
     1261 tcgaagaaga tgaacgggaa atagaacatc tgcgggctca gatagaaaaa ctgcgccgga
     1321 tgctgttcgg tacccgttct gaaaaactgc gtcgtgaagt tgaactggct gaggccctgc
     1381 tgaaacaacg tgaacaggac agcgatcgtt acagtgggcg ggaagacgat cctcaggttc
     1441 cccgccagtt gcgacagtcg cgccatcgtc gtccgttacc ggtacacctt ccccgtgaaa
     1501 tacaccgcct ggagccagaa gaaagctgtt gcccggagtg tggcggtgag ctggattatc
     1561 caggggaagt cagcgctgag cagctggaac tggtgagcag tgccctgaaa gtgatccgca
     1621 cagaacgggt aaaaaaagcc tgtacaaaat gtgactgcat cgttgaagca ccggcgccgt
     1681 cccgcccgat agagcgtggt atcgcgggcc ccggattact tgcccgcgtg ttaacgggaa
     1741 aatactgcga acatctgcca ctgtatcgtc agagtgaaat ctttgcccgc cagggtgtcg
     1801 aactgagccg ggccttactc tccaactggg ttgacgcgtg ctgccagtta atgacaccgg
     1861 tgaatgatgc cctgtaccgt tatgtaatga acacccgcaa ggttcacact gatgacacac
     1921 cggtaaaggt actggcaccg ggtcagaaaa aggcgaaaac agggcgtatc tggacgtatg
     1981 tccgggatga tcgcaatgtg ggttcgtcat ctcctccagc ggtctggttc gcgtactcgc
     2041 cgaaccggca ggggaaacac ccggagcaac acctccgccc cttccggggt atcctgcagg
     2101 cggatgcgtt cacaggttac gacaggttgt tcagtgcaga acgtgaaggt ggtgcactga
     2161 cagaagttgc gtgctgggcc catgcccggc gaaaaatcca cgatgtatac atcagcagca
     2221 aaagtgcgaa ggcagaagaa gccctgaagc gaatcagtga actgtacgcc atcgaggatg
     2281 aaatacgggg attaccggag tcagagcgtc tttccgtcag gcagcagcga agcaaagtgt
     2341 tactgacgtc gctgcatgaa tggatggtgg agaagaatgg tacgctgtcg aaaaaatcca
     2401 gactgggcga agcgttcagc tatgtactga atcagtggga tgccctctgt tattacagtg
     2461 atgacggtct ggcggaggcg gataataatg ctgcggaaag agcgcttcgt gcagtctgtc
     2521 tcggaaagaa aaactcttat gacctttgtc aaatattaag cccaaaaaga cctacgcagc
     2581 tccgggagct tcgttatatc gcggattaaa gaacctgaaa cagtcagact gtatcttgga
     2641 ttttacaaac tcttattcgg ggattttagc cccatgacga tttacgcagt gagctggcct
     2701 ctttctacgt gcaggtatta gcgcattctg gcttccctga attctttttc gaggatcact
     2761 cattccgtgt ctgactgctt cagggtttaa ttttgcttct gctgactgaa tgatgattgg
     2821 gcagcaagtg caatatccca cataaatcca gtcaactcac gagccaccgc cgtaatggct
     2881 acttgtgatt ttttcccttt tgctgtcaga gttcggtaac gagaacacaa tcgttgttga
     2941 gctttccatg caatatcttt ggaatattgt gttacagagt ccggtcggta atatataagc
     3001 catgcaccaa cttttgcagg tgtacgataa gaccaggcgg cttcgtaaag cagacgtctc
     3061 agttcgctgt ttccaacttt tgttattcct ctgggacgaa tactgtttcc actggaatat
     3121 tcacctggca ccagtcccag aaaagccatg agctgtttgg gattggaaaa tcgcgaaaaa
     3181 tcacccagtt cagcaaccag ggttattgca attagctgac caacaccttt tagagcctgc
     3241 aactggcaga ccagattgca cagagaccac tcaggtaaca ggcgagatat ttcctggtcg
     3301 agttgaagta tgcgatcttc aatctgctcc agactttgac aatagtgttg aaaagcaat
//
LOCUS       DAZKFU020000358         3358 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZKFU020000358 DAZKFU020000000
VERSION     DAZKFU020000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.360"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            704..1576
                     /locus_tag="ACHOQQ_RS23095"
                     /old_locus_tag="pgaptmp_004619"
     CDS             704..1576
                     /locus_tag="ACHOQQ_RS23095"
                     /old_locus_tag="pgaptmp_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4472769.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHTGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            1949..>3358
                     /locus_tag="ACHOQQ_RS23100"
                     /old_locus_tag="pgaptmp_004620"
     CDS             1949..>3358
                     /locus_tag="ACHOQQ_RS23100"
                     /old_locus_tag="pgaptmp_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4472770.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGIARDTLIYAGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGINRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNVGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNN"
BASE COUNT          873 a          875 c          889 g          721 t
ORIGIN      
        1 taaaataaca cttcttcgac aaacacaaaa acagccttca ttttacatca tcatccagtt
       61 tcagttaacc ccggacgagc gacagcgttt cagtacagga attaaaagcc agcttttgct
      121 cagtaaaaac acactcaact aataatatct cagcagatat tatcctgacc aggacacctc
      181 acattactga attctcttcc gacagtatca ctgcacaaat aaaaaagaac tggataaata
      241 ttcagaccgg aagaatggtg tcacaaaagc atttaatcat tacgcatgac caggaaggga
      301 aacacttaac cacatggtat caacagatat cattacgtgt gcactcttat aaaaaacaca
      361 cagaagaagt aagataatta taaacgtaaa tttaatatct cccactgaca tctcccccac
      421 aatacactga aaaatactca cagcagtaaa cacagacaca cctgataatc atgcagccga
      481 taaatatttt cccctttacg gagaaatcag cctgctgttt aacaataacg gcgtattttc
      541 atggaaggag atcacccagt aacggataag tcctgatctc cttcatgtta atacgcaaca
      601 atcccatcga ttcaaaacaa tttcagacag atattatctc cgtgaatccc ccgccacctt
      661 tccggtgcgc ggggttttgt cttttttcac cgggaataca tgtatgaatc cgtctgatgc
      721 cattgaggca attgaaaaac cgctctcctc cctgccttac tcgctttccc gtcacatcct
      781 ggaacatctg cgcaaactca cccgtcacga accagtgatt ggcattatgg gtaaaagcgg
      841 ggccggtaaa tcctcactct gtaatgcact gtttcagggg gaggtcaccc cggtcagtga
      901 tgttcacacc ggcacccggg aagtacggcg cttccgtctg agtggccacg gtcacagcat
      961 ggttatcact gacctgcccg gggtgggcga gagccgggac agggatgcag agtatgaggc
     1021 cctgtaccgt gacattctgc ctgaactgga cctggtactg tggctgatta aagccgatga
     1081 ccgtgccctg tctgtggatg agtatttctg gcgacacatc ctgcaccggg gacatcagcg
     1141 ggtactgttt gtggtgacgc aggccgacaa aacggagccc tgccatgaat gggatatggc
     1201 cggtattcag ccttctcctg cacaggcaca gaatattcgc gaaaaaacgg aggcggtatt
     1261 ccgtctgttc cggcccgtac atccggttgt ggccgtatcg gcccgcaccg gctgggaact
     1321 ggatacgctg gtcagtgcgc tcatgacagc gcttcccgac catgccgcca gtcccctgat
     1381 gacccgactg caggacgagc tgcgcacgga atctgtccgg tctcaggccc gtgaacagtt
     1441 taccggtgcg gtggaccgga tatttgacac ggcggagagc gtctgtattg cctctgttgc
     1501 acgcacggtt ctgcgtgccg tccgtgactc ggtggtctct gttgcccgcg ctgtatggaa
     1561 ctggattttc ttctgaacac tctgccaggg gaaatgcctg attacattgc gttaaccaac
     1621 tgaaaacatg acacaaaccc ctcatccccc tcctgtttct cttgcgtgac cgctctgctg
     1681 tcaatagaat aaaacgatcg ataaaacagg tatcgatcgt tactatcgat cgtttatagt
     1741 ggttaatggt ctgcgaaaag gcagctgaat ctcttcatca tgcagaacgg aattgcacac
     1801 aacggactga tacttctctg gcggatgaga gaggggaagc atttatgtcc gggaaaaacc
     1861 actttatctg accatgctgt tttgtacctg ccggtatcca catttgtggg taccggcttt
     1921 tttattcacc ctctgtaagg aaaagctgat gaaacgacat ctgaacacca gctacaggct
     1981 ggtatggaat cacattacgg gcaccctggt ggtggcctcc gaactggccc gctcacgggg
     2041 aaaacgcgcc ggtgtggcgg ttgcgctgtc tcttgctgct gtcacatcag tcccggcact
     2101 ggctgctgac acggttgtac aggcgggaga aaccgtgaac gacggaacac tgacaaatca
     2161 tgacaaccag attgtcctcg gtacggccaa cggaatgacc atcagtaccg ggctggaact
     2221 ggggccggac agtgaagaaa acaccggtgg gcaatggata cagaatggcg ggatagccgg
     2281 aaacaccact gttaccacaa atggtcgtca ggtcgtgctg gaggggggaa cagccagtga
     2341 tacggttatt cgtgacggcg ggggacagag cctgaacggg ctggcggtga acaccacgct
     2401 gaataacaga ggcgagcagt gggttcatga gggcggggtt gccaccggta caattatcaa
     2461 ccgcgacggt taccagagcg ttaaaagtgg cgggctggca acaggaacca tcatcaacac
     2521 cggcgcagaa ggcggcccgg attctgacaa ctcgtacacg ggtcagaagg tccagggaac
     2581 agcagaatcc accaccatca acaaaaatgg acggcagatt atcttatttt ccgggatagc
     2641 ccgtgacact ctcatttacg caggtggtga ccagtcggta cacggaaggg ccctgaatac
     2701 cacactgaat ggcggttacc agtatgtgca caaggacgga cttgcgctga acacggtaat
     2761 taacgagggg ggctggcagg ttgttaaggc aggtggtgct gtcggtaaca ccaccataaa
     2821 tcagaacggt gaactgaggg tacatgccgg cggggaagcc actgcagtca cccagaacac
     2881 gggcggtgca ctggttacca gtacggctgc aaccgttacc ggcataaacc gccttggagc
     2941 attctcggtt gtggagggta aagctgataa tgtcgtactg gaaaatggcg gacgcctgga
     3001 tgtgctgacc ggacacacag ccactaatac ccgcgtggat gatggcggaa cgctggatgt
     3061 ccgcaacggt ggtaccgcca ccaccgtatc catgggaaat ggcggtgtac tgctggccga
     3121 ttccggtgcc gctgtcagtg gtacacggag cgacggaaag gcattcagta tcggaggcgg
     3181 tcaggcggat gccctgatgc tggaaaaagg cagttcattc acgctgaacg tcggtgatac
     3241 ggccacggat accacggtaa atggcggact gttcaccgcc aggggcggca cactggcggg
     3301 caccaccacg ctgaataacg gcgccatact taccctttcc gggaagacgg tgaacaac
//
LOCUS       DAZKFU020000359         3356 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZKFU020000359 DAZKFU020000000
VERSION     DAZKFU020000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.361"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(361..783)
                     /locus_tag="ACHOQQ_RS23105"
                     /old_locus_tag="pgaptmp_004621"
     CDS             complement(361..783)
                     /locus_tag="ACHOQQ_RS23105"
                     /old_locus_tag="pgaptmp_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HGU4472771.1"
                     /translation="MRSAVARLLLIPLITATIVLTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGVKDKAAGVSAETAQQRIKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            904..1182
                     /locus_tag="ACHOQQ_RS23110"
                     /old_locus_tag="pgaptmp_004622"
     CDS             904..1182
                     /locus_tag="ACHOQQ_RS23110"
                     /old_locus_tag="pgaptmp_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472772.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRASLAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            complement(1174..1464)
                     /locus_tag="ACHOQQ_RS23115"
                     /old_locus_tag="pgaptmp_004623"
     CDS             complement(1174..1464)
                     /locus_tag="ACHOQQ_RS23115"
                     /old_locus_tag="pgaptmp_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472773.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            1703..3199
                     /locus_tag="ACHOQQ_RS23120"
                     /old_locus_tag="pgaptmp_004624"
     CDS             1703..3199
                     /locus_tag="ACHOQQ_RS23120"
                     /old_locus_tag="pgaptmp_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HGU4472774.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGS
                     GNGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPEAGGNSDV
                     QGGYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVG
                     SEKAKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVD
                     VEQFHYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNE
                     EPQRQNQAKKAAYDVFYSFSMNRDRIQSDVLNKAAELISDMGNKIGDYLGESYKSLAR
                     EIADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADHDALVNAWRSRDAQDIANK
                     LGNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVESWVLSGMAASVAMTLLS
                     AIIGSFTLPALALTAINIAGIMGISYLASYIDASVAEKINNKLVHFTH"
BASE COUNT          959 a          580 c          826 g          991 t
ORIGIN      
        1 ttaaaatgct tacgccatcg gtatgtttta tattttgaga tagcactacg atagtgtttg
       61 agtagaaact aatgggtact cccagatgca tattcaggtt gatggaaggg ggtaaaaatc
      121 cccgtgctga accttccatc cggagggagg aaacttatca ttttaacctg ttcgtccgtc
      181 ggaatagtcg aaatcgatcg cgcatcgctt ttgtgaatag ttatgcaagt acctgaaaac
      241 gattctgacg cattttttcg gtttagcctg gggtttcctt gtctttttgc gtttttgcgt
      301 cagaacgcgt atgagagcgt ttcagcggta cgtacaatta agggattatg gtaaatcccc
      361 ttactgtctg ccctcgtatc catcgagata aaccgcagaa atggtgtctg ccattagttt
      421 catttttttc ggcgacggca tatcgctgtt tgtgtatttt ctgccagcaa aggttgttgt
      481 gctccggagt gagtgaaaaa atttttctga gtggaattct tttatacgtt gctgtgccgt
      541 ttctgctgat actccggctg ctttgtcttt cacccccata tcccggaact gctgaaagaa
      601 cgggagcatc atacgtatgg tgtctgcttt ctgggtgtag aagttaggat caaatccatc
      661 atctgaagca taaacataat gccgggtatg tcgttccagg ctggtctttg gtgtgcatcc
      721 tgtaagaacg attgttgctg ttatcagagg tatcagaagc agcctggcaa cggcagatct
      781 cataaacatc tccttgcgtt tttaatcttg atactcaggg agttatattt ccgaagagta
      841 tctcttatca tacgtcggtt taccccaatg tgatttgatg gtcaatgagt caggaataat
      901 cccgtgaaaa aatgtgtgcc agtcctgctt tttagtgttc tttctgcttc ggttgccagt
      961 catgcggaca gcaatgtttt tcaacaaaag gccgagaaga caaaaaatgg cattgttgcg
     1021 gatgttaatg ccagacagaa aatgttcagt aacagactga agatacaact tcagatcgat
     1081 gagttgaggg catctctggc tggcaccaga gatgcccaaa aaagagagga tttgcgtaaa
     1141 aaactggagt ctctggagcg gcaaaagaaa tccttacgct gatgggggcg tattttcccc
     1201 ggtttcttcc ataacctttt taccgttcag atatttacgg gcgaggcgaa tgcgtcttgc
     1261 ctggtatacc gccgagtaag cctgggtggg attcacaccg ttactgattt cactgaccac
     1321 atggcgctga tcagccagtg tcagcgtgta aacctgtttc tggatgaggt cacgggcttt
     1381 gtcatgctca tatccggcag caacaagggt gtcgagaagt cgtttgtctg ttttgttcag
     1441 tttatggcgg ggtgatttgc tcacatcgtt ctccgttttt catgatccaa cagttttata
     1501 tctcatcctg atttgctccg gtgttctgtt tcgacgttct caatgcctgc ctccccatag
     1561 tgtgatcctt attgcaaaaa tgaaaatcca tgctcttgac atggacaatg gtgagtagta
     1621 ggtttttctg tacataaaac cagtggttat atgtacagta tgttgttttt aatttattgt
     1681 tttaattatc aaagaggaat ttatggctaa agaactaagt ggatatggcc caactgctgg
     1741 tgagtctatg gggggaacag gggcgaatct gaatcagcaa ggtggaaata ataatagtaa
     1801 tagtggggtt cactggggtg gtggttcagg ctctggtaat ggaggtggtg aacatggaag
     1861 tcagacgggg tggggatgga gtaaaaccaa taatccggat gtgccgcctt atgttgatga
     1921 taatgggcag gtgcgcatta ccattacaaa tggtctggta aaaacgcctg tatatggagt
     1981 tcctgaggct ggcggaaata gtgatgttca ggggggctat attcctgaaa atcctaatga
     2041 tgaagttgct cgtaagtggg ataaaaacaa cttaccgcga gagattgatg tgagtatcga
     2101 tggttttaaa tatcgtgtaa cgcttaatga taatggtaga gctatcggta ttcttcgaac
     2161 aggtgtccgt ccttacgtag ggagtgaaaa agctaaggcg ggtattatgg aaaaaatcaa
     2221 tcataaaaca cctgaggaga tatatgaggc gttaggtttt aataaagatg aatctcagcg
     2281 gcaggaaaaa gcaaaacaac aggctgaaga tgcgtgggat cgtttgcctc ctaatgtgag
     2341 aaaatttgat gttgatgttg aacagttcca ctatttagtg gtgcttgatg attatggtaa
     2401 tgttttatct gtaactagaa cagctgttcg tccttatgta gggagtgaaa aagctaaggc
     2461 tggtattatg gataaagtcg accataagac cccggaagag atatacgagg cattaggatt
     2521 taataatgaa gagcctcaac gacagaatca agctaaaaaa gctgcttatg atgtatttta
     2581 ttctttcagt atgaaccgtg accggattca gagtgatgtg ctaaataaag cagctgaact
     2641 tatatctgat atgggtaaca agatcggtga ttatcttggt gaatcttata agtcgttagc
     2701 aagagaaatt gctgatgata tcaaaaattt ccaggggaaa accatccgta gctacgatga
     2761 tgcaatggag tcattaaata aaattttgtc taatcctagt tttaaagtta acagagcaga
     2821 ccatgatgca ctggtgaacg cctggcgaag tcgtgatgct caggatatag caaataaact
     2881 ggggaatatc agtaaggctt tcaaagctgc cgatgtaatt atgaagattg agaaggtcaa
     2941 ggagaaaagc atcgagggat atgaaactgg aaactggggg ccgctgatgt tggaggttga
     3001 gtcttgggtt ctcagtggta tggccgcaag cgttgccatg acattgctta gtgctataat
     3061 tggatcattc acactaccgg cgttagcatt aactgcaata aatattgcag gtattatggg
     3121 aattagttac cttgcatcat atattgatgc aagtgtggct gaaaaaataa ataataaatt
     3181 ggttcatttt actcattgat agaaaaagag aggtgatcat atcacttctc tttaaacgac
     3241 ctataggaac caatacaaaa ccaaaaatac aaatatgaga atataaaaat aatggaatgt
     3301 aagctaatgt aaaaaaacga taggaggatt atatttacag acatcagttt tagcat
//
LOCUS       DAZKFU020000360         3349 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZKFU020000360 DAZKFU020000000
VERSION     DAZKFU020000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.362"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            58..984
                     /gene="allS"
                     /locus_tag="ACHOQQ_RS23125"
                     /old_locus_tag="pgaptmp_004625"
     CDS             58..984
                     /gene="allS"
                     /locus_tag="ACHOQQ_RS23125"
                     /old_locus_tag="pgaptmp_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGU4472775.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            1053..2147
                     /gene="mnmH"
                     /locus_tag="ACHOQQ_RS23130"
                     /old_locus_tag="pgaptmp_004626"
     CDS             1053..2147
                     /gene="mnmH"
                     /locus_tag="ACHOQQ_RS23130"
                     /old_locus_tag="pgaptmp_004626"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGU4472776.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHAAGIDYPLVAGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            2263..2628
                     /gene="ylbG"
                     /locus_tag="ACHOQQ_RS23135"
                     /old_locus_tag="pgaptmp_004627"
                     /pseudo
     CDS             2263..2628
                     /gene="ylbG"
                     /locus_tag="ACHOQQ_RS23135"
                     /old_locus_tag="pgaptmp_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3149..>3349)
                     /locus_tag="ACHOQQ_RS23140"
                     /old_locus_tag="pgaptmp_004628"
     CDS             complement(3149..>3349)
                     /locus_tag="ACHOQQ_RS23140"
                     /old_locus_tag="pgaptmp_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472777.1"
                     /translation="TLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPGQGDSVNLFFVG
                     EINVTHYFITNIGAGLPDACAE"
BASE COUNT          842 a          807 c          856 g          844 t
ORIGIN      
        1 taaatcagag gataagaaaa tcacgcaact tgcggattta aaaggggata cctgaagatg
       61 ttcgatccag aaaccttgcg gactttcatt gcggttgctg aaacaggaag tttttcaaaa
      121 gcggcagaac gattatgtaa aaccacggcg acgatcagtt atcgcattaa acttctggaa
      181 gagaataccg gagtagcgct gtttttccgt acgactcgta gcgtaacgtt gacagcggct
      241 ggcgagcatc tactttccca ggccagagac tggctgagct ggctggaaag tatgcccagc
      301 gagctgcaac aggtgaatga tggcgtggaa cgccaggtga atattgtcat caacaacctg
      361 ctctataacc cccaggccgt cgcccagttg ctggcgtggc tgaatgagcg ttaccccttt
      421 acccagtttc acatctcccg acaaatctat atgggcgtct gggactcgct attgtacgaa
      481 ggtttttcgc tggctatcgg cgtcacggga actgaggcgc tggcaaatac ctttagtctt
      541 gatcccttag gatcggtgca atggcgattt gtcatggcgg cggatcatcc gctggcgaac
      601 gttgaagagc cgctaacaga agcgcagttg cggcgctttc cggcggtcaa tattgaagac
      661 agcgcccgca ccttaaccaa acgcgtcgcc tggcgattgc cgggtcaaaa agagattatt
      721 gttcccgata tggaaacgaa aatcgccgcc catctggcgg gcgttggcat tggttttttg
      781 ccaaaatcgc tttgccagtc aatgatcgat aatcaacaac tggtcagccg ggtaatccca
      841 acgatgcgcc ctccttcgcc attgagtctg gcatggcgca aatttggcag cggcaaagcg
      901 gtagaagata ttgtgacctt gtttacccag cgcaggccgg aaatcagcgg atttttagaa
      961 attttcggca acccacgcag ttaaaaatca agtttccttg cgcgttggcg cgtaaaatag
     1021 cgcgcaagct gatttgaggt tcacttttaa ctatgcaaga gagacacacg gaacaggact
     1081 atcgtgccct gctgattgct gatacgccca ttattgatgt tcgcgcccct atagagtttg
     1141 agcaaggcgc aatgcccgcc gctatcaatc tgccgttaat gaataacgat gaacgcgccg
     1201 ccgttggcac ctgctataaa cagcaaggct cagacgcagc gctggcgctg ggacataaac
     1261 tggtggcggg tgaaattcgt cagcagcgca tggacgcctg gcgggcagcg tgcctgcaaa
     1321 atccgcaagg tattctctgc tgcgcccgtg gcggtcagcg ctcacatatt gtgcaacgct
     1381 ggttgcatgc agcggggatt gattatccgc tggtggcagg cggttataag gcactgcgcc
     1441 agaccgcgat tcaggcgact attgaactgg cacaaaaacc gatagtgctg attggcggtt
     1501 gtaccggcag cggtaaaacg ctgttagtgc agcaacagcc gaacggtgtt gatctggaag
     1561 ggttggcgcg tcatcgcggt tcggcgtttg gtcgcacgtt acaaccacaa cttagccagg
     1621 cgagttttga aaacctgctg gctgccgaaa tgctaaaaac cgacgcccgt cagaatttgc
     1681 gcctgtgggt gctggaagac gaaagccgga tgatcggttc gaatcacctg ccggaatgcc
     1741 tgcgcgagcg aatgactcag gcggcgattg cggtggtaga agatccgttt gagatccgtc
     1801 ttgagcgcct gaacgaagag tatttcttgc gtatgcatca tgattttacc cacgcgtacg
     1861 gcgacgaaca gggctggcag gaatattgcg aatacctgca tcacggactt tcggctatta
     1921 agcgtcggct ggggctacag cgatataacg aactggctgc aaggctggat gcagcactga
     1981 caacgcaact caccaccggc agcaccgacg gtcatctggc ctggctggtg ccgttacttg
     2041 aagaatatta cgacccgatg tatcgctatc agcttgagaa aaaagcggaa aaagttgtct
     2101 ttcgcggtga gtgggcggaa gtggcggaat gggttaaggc gcagtgatga gggacgtatt
     2161 tgtattagtc gcgacttgat ctgacacatg gccttgaaag gttgagagtt accggttttg
     2221 atatgagtgt cgaatcctta tccaaaacaa gaggtaactc tcatgcttca tactgccaat
     2281 cccgtcatca aacacaaagc cggtttgctc aatctggctg aagaactcag caacgtatca
     2341 aaagcctgta aaatcatggg cgtttcgcgt gatacgtttt atcgttaccg cgaactggtc
     2401 gctgaaggcg gtgtggatgc gcagattaat cgtagtcgcc gcgctcctaa ccttaagaac
     2461 cgtaccgatg aggcaactga acaggctgtt gttgattacg ccgtcgcttt cccgacacat
     2521 ggtcagcacc gggccagcaa cgaactgcgt aaacaggggg tttttatctc cgacagtggt
     2581 gtccgttctg tctggttact ccataacctt gagaatctca aaagacgata ctgaacaaat
     2641 tttatcaggt gacgttccgt aaaaagttgt atggagttct cgatgcagtc ggcgcgacta
     2701 tcagtacccc cgctgaaccc tggactatcg ggccgccgta ctggggcatg tcaccctggc
     2761 gtgccgctgg ttttccgcta atggtttatt tcctctttct ttcccggcaa gaatttattt
     2821 taatccggaa ggattattta ttttcagata gggactcaca ttaaacacgt aacattttaa
     2881 ttaatgcatg gctgaatatg tattacacaa tgtcagatat cgtaaaagac cagtgcaatt
     2941 ctatgtaaac tctctgactg ttcattttat ttgttgtttc agggtcggaa ctcaccctca
     3001 ataaacaact gaatacgagt agatattcat cgtctgagct atatggcttt acacaatagc
     3061 cgacatgtct tttctcagtc aggaatttta cttttcctca ctacgaagtt gacgacttcg
     3121 atatgggata gactcttaat tcaagcaatt actctgcaca agcatcaggc aatccagctc
     3181 caatatttgt tataaaataa tgcgtaacat taatttcacc aacaaaaaaa agatttacgc
     3241 tatccccttg ccctggtatt ttaccatatt tacaccacac tttttttgta tctggatcat
     3301 tctttgatac gattttcgcc acttgttccc tggccctcac cgtcaatgt
//
LOCUS       DAZKFU020000361         3321 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZKFU020000361 DAZKFU020000000
VERSION     DAZKFU020000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.363"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(2..88)
                     /locus_tag="ACHOQQ_RS23145"
                     /old_locus_tag="pgaptmp_004629"
     tRNA            complement(2..88)
                     /locus_tag="ACHOQQ_RS23145"
                     /old_locus_tag="pgaptmp_004629"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Leu,seq:cag)
     gene            complement(117..203)
                     /locus_tag="ACHOQQ_RS23150"
                     /old_locus_tag="pgaptmp_004630"
     tRNA            complement(117..203)
                     /locus_tag="ACHOQQ_RS23150"
                     /old_locus_tag="pgaptmp_004630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Leu,seq:cag)
     gene            complement(471..1502)
                     /gene="rsmC"
                     /locus_tag="ACHOQQ_RS23155"
                     /old_locus_tag="pgaptmp_004631"
     CDS             complement(471..1502)
                     /gene="rsmC"
                     /locus_tag="ACHOQQ_RS23155"
                     /old_locus_tag="pgaptmp_004631"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGU4472778.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGIEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1605..2018
                     /gene="holD"
                     /locus_tag="ACHOQQ_RS23160"
                     /old_locus_tag="pgaptmp_004632"
     CDS             1605..2018
                     /gene="holD"
                     /locus_tag="ACHOQQ_RS23160"
                     /old_locus_tag="pgaptmp_004632"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGU4472779.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1987..2433
                     /gene="rimI"
                     /locus_tag="ACHOQQ_RS23165"
                     /old_locus_tag="pgaptmp_004633"
     CDS             1987..2433
                     /gene="rimI"
                     /locus_tag="ACHOQQ_RS23165"
                     /old_locus_tag="pgaptmp_004633"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4472780.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2448..3125
                     /gene="yjjG"
                     /locus_tag="ACHOQQ_RS23170"
                     /old_locus_tag="pgaptmp_004634"
     CDS             2448..3125
                     /gene="yjjG"
                     /locus_tag="ACHOQQ_RS23170"
                     /old_locus_tag="pgaptmp_004634"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGU4472781.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
BASE COUNT          785 a          906 c          881 g          749 t
ORIGIN      
        1 ttggtgcgag gggggggact tgaaccccca cgtccgtaag aacactaaca cctgaagcta
       61 gcgcgtctac caattccgcc accttcgccc agtgcgagca atatcaacgt ggtttttggt
      121 gcgagggggg ggacttgaac ccccacgtcc gtaaggacac taacacctga agctagcgcg
      181 tctaccaatt ccgccacctt cgcatgccat caattcttaa aaagaattgc taccacggag
      241 gcgcattcta gtggttttca gcttttcgtc aatagttaat tatcgccgga ggtgtaattg
      301 ctggaaaaat gtccatcagg aaactagcgt gcaggtttgg taggcatgcg ggggcagatg
      361 ccagatgcga cgctggcgcg tcttatctgg cctacgaagg gctaacgtgc aggttttgta
      421 ggtcggataa ggcgttcacg ccgcatccga cacagtattc ggcgggatac ttaacctttc
      481 ttcgcctggc gggtcataat ggcgcgatac accttgaagc gccctgtttg cgcgataact
      541 tcatggaagc caaatgtctc atccagcacg tccggataag gcaggaaggc gttcgctaca
      601 attcgcagct cgccgccgct attaagatga cgcaccgcgc cgcgaatcag cgtttgcgcc
      661 gcatccaggc tggtttgcat cccatcgtgg aacggcggat tggagatgat catatcaaaa
      721 cgacctttta cctcggaaaa gacgttgctg gcaaagactt caccttcaat accgttggcg
      781 gcaagcgtcg cgcggctggc ttcaactgct ggcgcagaga catcgcacaa ggtgagacga
      841 attttcggcg aatggcgcgc aaaggcaact gaaagcacac ccgcgccaca gccgacatcc
      901 agcactttac ctttcgtgtg cggagttaac gtcgagagca gcaactggct accgacatcc
      961 agaccgtcgc ggctaaacac gccaggcagc gttttgaccg tcaggccatc gacgctgtat
     1021 tcgccccaga atttatccgc atcaaatact ggctgttttt ccagacgacc aaaatagagt
     1081 ccacagcgac gagcgctgtc gactttattc aacggcgcat aatctgccag catctgctcg
     1141 gcgctgcgca cgccgctgcg gttctcgcca acgacaaaaa tatctgtccc cactggcagc
     1201 agagaaagta aattcatcaa ctggaactgg gcttccggtt tgttcttcgg ccagtagtaa
     1261 atcagcgtat cgcaatctgc gacgtcatcc gccgtggcga ccagactgaa acgggcgtta
     1321 tcccccatct ggcggcttaa tacctgccag tggtggaatt gctgggtatg agcacggctg
     1381 gccgcggtat ctaaacgcgc gggcaggtca tcctgtaagt ctccggcaaa cagaatacgg
     1441 ctttgttcga aatcatcact gtgacgcagc aagacttcac ttgccggggt aaatgcagac
     1501 atggaatgct cctcaattga tactggcggc gattatagcc atatgttggc gcggtatcga
     1561 cgaatttgct atatttgcgc ccctgacaac aggagcgatt cgctatgaca tcccgacgag
     1621 actggcagtt acagcaactg ggcattaccc agtggtcgct gcgtcgccct ggcgcgttgc
     1681 agggggagat tgccattgcg atcccggcac acgtccgtct ggtgatggtg gcaaacgatc
     1741 ttcccgccct gactgatcct ttagtgagcg atgttctgcg cgcattaacc gtcagccccg
     1801 atcaagtgct gcaactgacg ccagaaaaaa tcgcgatgct gccgcaaggc agtcgctgca
     1861 acagctggcg gttgggtact gacgaaccgc tatcactgga aggcgctcag gtggcatcac
     1921 cggcgctcac cgaattacgg gcaaacccaa cggcacgcgc cgcgttatgg caacaaattt
     1981 gcacatatga acacgatttc ttccctcgaa acgactgatt taccagcggc ttaccacatt
     2041 gaacaacgcg cccacgcctt tccgtggagt gaaaaaacct ttgccagcaa ccagggcgag
     2101 cgttatctca actttcagtt aacgcaaaac ggcaaaatgg cggcgtttgc gattacgcaa
     2161 gtggtgctgg atgaagctac attgttcaat attgcggtcg atcctgacta tcagcgtcag
     2221 ggattgggaa gggcgctgct ggaacatctg atcgacgaac tggaaaaacg cggcgtggcg
     2281 acgctctggc tggaagtccg tgcctcaaac gctgccgcca ttgccctgta cgaaagttta
     2341 ggctttaacg aggcgacgat tcgccgcaat tactacccca ccacggacgg tcgcgaagac
     2401 gccatcatca tggcgttgcc aatcagtatg taagacaagg tggaataatg aagtgggact
     2461 ggattttctt tgatgccgat gaaacgttgt ttacctttga ctcattcacc ggcctgcagc
     2521 ggatgtttct tgattacagc gtcaccttta ccgctgaaga ttttcaggac tatcaggccg
     2581 ttaacaagcc actgtgggtg gattatcaaa acggcgcgat cacttcatta cagcttcagc
     2641 acgggcggtt tgagagctgg gccgaacggc tgaacgtcga gccgggtaaa ctcaacgaag
     2701 cctttattaa tgcgatggcg gaaatctgta cgccgctgcc gggcgcggtt tctctgctta
     2761 acgccattcg tggcaacgcc aaaatcggca tcatcaccaa cggctttagc gccttgcagc
     2821 aagtgcgtct ggaacgtacg ggcctgcgtg attacttcga tttgctggtg atttccgaag
     2881 aagttggcgt tgccaaaccg aataagaaaa ttttcgatta tgcgctggaa caggcgggca
     2941 atcctgaccg ttcacgcgtg ctgatggttg gcgacactgc cgagtccgat attctcggtg
     3001 gcatcaacgc cgggcttgcg acctgctggc tgaatgcgca caatcgcgag caaccagaag
     3061 gcatcgcgcc cacctggacc gtttcttcgt tgcacgaact ggagcagctc ctgtgtaaac
     3121 actgattgcc tcccccccgt tgatgggtaa aatagccgca atttttcgtt ttcaacaagc
     3181 gcggcgcgat gccgcttact caagaagaaa gaattatgac gttgtctcct tatttgcaag
     3241 aggtggcgaa gcgccgcact tttgccatta tttctcaccc caataattaa gcccaaatta
     3301 aagctctttt actctttcaa a
//
LOCUS       DAZKFU020000362         3319 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZKFU020000362 DAZKFU020000000
VERSION     DAZKFU020000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.364"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            333..1649
                     /gene="shiA"
                     /locus_tag="ACHOQQ_RS23175"
                     /old_locus_tag="pgaptmp_004635"
     CDS             333..1649
                     /gene="shiA"
                     /locus_tag="ACHOQQ_RS23175"
                     /old_locus_tag="pgaptmp_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HGU4472782.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            1751..3205
                     /gene="amn"
                     /locus_tag="ACHOQQ_RS23180"
                     /old_locus_tag="pgaptmp_004636"
     CDS             1751..3205
                     /gene="amn"
                     /locus_tag="ACHOQQ_RS23180"
                     /old_locus_tag="pgaptmp_004636"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGU4472783.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYIRSGELPDE
                     NARKQGLFVYPSLTVTWDGGTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          763 a          815 c          805 g          936 t
ORIGIN      
        1 caatgatgct aaaaacatac ctaacctcgc ctccctactg gttataatgc aatgcagtct
       61 atcagactca tcagggtgcc attttgtgca tatgcggact tttatgtttc atatctcgaa
      121 cctgtgggtc ctctgcttaa tccttaaaca acaccagcaa ctcccgcgct ttcatcttcc
      181 agcgaatttt tcatgttgcc gctaatcagc cataaaatca tttgcagatg cgctctgtcg
      241 aggtagtctc ataaggttcg tttatagatc gacggcaatg tgagttacct tttccatact
      301 aattataaaa agacagtaca aacaggatca ttatggactc cacgctcatc tccacccgtc
      361 ccgatgaagg gacgctttcg ttaagtcgcg cccgacgagc tgcgttaggc agtttcgctg
      421 gtgccgtcgt cgactggtat gattttttac tctatggcat caccgccgca ctggtgttta
      481 atcgcgagtt tttcccgcaa gtaagcccgg cgatgggaac gctcgccgca tttgctacct
      541 ttggcgtcgg attccttttc cgtccgctcg gcggtgtcat tttcggtcac tttggcgacc
      601 gactgggacg taagcgcatg ttaatgctga ccgtctggat gatgggtatc gcgacagcct
      661 tgattggtat tcttccttca ttctcgacca ttgggtggtg ggcacctatt ttactggtga
      721 cgctgcgtgc cattcaggga tttgccgtcg gcggcgaatg gggaggcgcg gcgttgcttt
      781 ccgttgaaag tgcaccaaaa aataaaaaag ccttttacag tagcggtgta caagttggct
      841 acggtgtagg tttactgctt tcaaccggac tggtttcatt gatcagtatg atgacgactg
      901 acgaacagtt tttaagctgg ggctggcgta ttcctttcct gtttagcatc gtactggtac
      961 tgggagcatt gtgggtgcgc aatggcatgg aggagtccgc ggaatttgaa caacagcaac
     1021 attatcaagc tgccgcgaaa aaacgcatcc cggttatcga agctctgtta cgacatcccg
     1081 gtgctttcct gaagattatt gcgctacgac tgtgcgagtt gctgacgatg tacatcgtta
     1141 ccgcctttgc acttaattat tcaacccaga atatggggtt accgcgcgaa cttttcctta
     1201 atattggttt gctggtaggt ggattaagct gcctgacaat tccctgtttt gcctggcttg
     1261 ccgatcgttt tggtcgccgc agggtttata tcacaggcgc gttgatcgga acgttgagcg
     1321 catttccttt ctttatggcg cttgaagcac aatctatttt ctggatagtt ttcttctcca
     1381 taatgctggc aaacattgcg catgacatgg tggtgtgtgt gcaacaaccg atgtttaccg
     1441 aaatgtttgg tgccagttat cgctatagtg gtgctggagt cggttatcag gttgccagtg
     1501 tggttggcgg tggatttaca ccttttattg ccgctgcgct catcacttac tttgccggga
     1561 actggcatag cgtcgccatt tatttgctgg ctggatgtct aatttccgca atgaccgctt
     1621 tgttgatgaa agacaatcaa cgcgcttgat agtctggcga agatcatccg atcttcgcct
     1681 tacacttttg tttcacattc ctgtgacata ctatcggatg tgcggtaatt gtatggaaca
     1741 ggagacacac atgaataata agggctccgg tctgacccca gctcaggcac tggataaact
     1801 cgacgcgctg tatgagcaat ctgtagtcgc attacgcaac gccattggca aatatattag
     1861 aagtggcgaa ttacctgatg aaaacgcccg caaacaaggt ctttttgtct atccatcact
     1921 gaccgtaacc tgggacggtg gcacaaccaa tccccccaaa acgcgcgcat ttggtcgttt
     1981 tactcacgca ggcagctaca ccaccacgat tactcgccct actctctttc gttcgtatct
     2041 taatgaacaa cttacgttgc tgtatcagga ttatggtgcg catatctcgg tgcaaccctc
     2101 gcagcatgaa atcccttatc cttatgtcat cgatggctct gaattgacac ttgatcgctc
     2161 aatgagcgct gggttaactc gctacttccc gacaacagaa ctggcgcaaa ttggcgatga
     2221 aactgcagac ggcatttatc acccaactga attctccccg ctatcgcatt ttgatgcgcg
     2281 ccgcgtcgat ttttccctcg cgcggttgcg ccattatacc ggtacgccag ttgaacattt
     2341 tcagccgttc gtcttgttta ccaactacac acgttatgtg gatgaattcg ttcgttgggg
     2401 atgcagccag atcctcgatc ctgatagtcc ctacattgcc ctttcttgtg ctggcgggaa
     2461 ctggatcacc gccgaaaccg aagcgccaga agaagccatt tccgaccttg catggaaaaa
     2521 acaccagatg ccagcatggc atttaattac cgccgatggt cagggtatta ctctggtgaa
     2581 tattggcgtg ggaccgtcaa atgctaaaac catctgcgat catctggcag tgctacgccc
     2641 ggatgtctgg ttgatgattg gtcactgtgg cggattacgt gaaagccagg ccattggcga
     2701 ttatgtactt gcacatgctt atttacgcga tgatcacgtt cttgatgcgg ttctgccgcc
     2761 cgatattcct attccaagca ttgctgaagt gcaacgtgcg ctttatgacg ccaccaagct
     2821 ggtgagcggc aggcccggtg aggaagtcaa acagcggcta cgtactggca ctgtggtaac
     2881 cacagatgac aggaactggg aattacgtta ctcagcttct gcacttcgtt ttaacttaag
     2941 ccgggccgta gcaattgata tggaaagtgc aaccattgcc gcgcaaggat atcgtttccg
     3001 cgtgccatac gggacactac tgtgtgtttc agataaaccg ttgcatggcg agattaaact
     3061 tcccggccag gctaaccgtt tttatgaagg cgctatttcc gaacacctgc aaattggcat
     3121 tcgggcaatc gatttgctgc gcgcagaagg cgaccgactg cattcacgta aattacgaac
     3181 ctttaatgag ccgccgttcc gataataagt tctgaacctt cttatcaaaa atagatgtga
     3241 aggattaagt aagacatctg gcaaatagcc tgcaataaag caggctgttt gcttccttag
     3301 gcggatagga gttttatta
//
LOCUS       DAZKFU020000363         3234 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZKFU020000363 DAZKFU020000000
VERSION     DAZKFU020000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.365"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1006..3054
                     /gene="ireA"
                     /locus_tag="ACHOQQ_RS23185"
                     /old_locus_tag="pgaptmp_004637"
     CDS             1006..3054
                     /gene="ireA"
                     /locus_tag="ACHOQQ_RS23185"
                     /old_locus_tag="pgaptmp_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HGU4472784.1"
                     /translation="MKNKYIIAPGIAVICSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
BASE COUNT          995 a          671 c          695 g          873 t
ORIGIN      
        1 gtcagcatgt cttcatcctc atcagcagct cttcagtgga taacatccac agttacacct
       61 gcatttacgc gcaacaaaaa caccagatga agattttatt cattcagctc cgctaaaaat
      121 taatcttgcc gtggaatatc gggaaaacag gaagttacgt taaaagccga aggaaaaact
      181 cacaatccct tcttcaatca gcctctgaaa atcggctttt cagcatgtct tcccccattg
      241 ttcgtatacc ctgttcgatg gattattatt gatccttatg atcgcttcac gtaaacacga
      301 ttctggctcc acaccattct ggacgcgatt gttaccaaaa aagacaacca tcacaagacg
      361 tcacagacgg tgcgtgcgct tactcctgta tatctggcgc aacagaatct accagtgtaa
      421 actctctatg cttttgcgca gaagcagtgg gcctgggttc gcttatgtcc tgcatctaca
      481 agcgaatgac tgaccagaga ctcccatatc tggtcagtcg cccattttta ttttccctat
      541 acactcagac tacaatagcc gtaacaaaca gcataccccc agtcatagtg gcccctttat
      601 cttcttatgg cttgtctgtg gtactggaga tagagcaact gtgggagtac tgttatggcc
      661 cccggtaaga aaaactcttg cttaatgtac agcagaactg ttcccagaaa gaagcctgtg
      721 catattttca tagaaggaga tcacccataa cggcgttttt cacggtaata cacgccaggt
      781 aaagaatgct ggattatcaa tcaaagctcc gcacaggaag cgcttatgcc gtagagcgcc
      841 ctctggagaa aagtgctact tcttgtcgat tcagactagt tcaggaaatt atacgaaaat
      901 attacctgaa tgagctaaat gttatttacc ttcctttctg ttcattgtaa attttttctt
      961 gctaacgcaa atcattatca ttacgatttt agaaaaggga aatttatgaa gaacaaatat
     1021 atcattgctc cgggcattgc cgtgatatgt tctgcagtca tatcatcagg ttatgccagt
     1081 tctgataaaa aagaagatac gcttgttgtt actgcctccg gcttcactca gcaactcaga
     1141 aatgcaccgg ccagtgtttc agtcattact tcagagcaac tgcaaaaaaa accggtttca
     1201 gacctggtcg atgcggtaaa agatgttgaa gggattagta tcaccggtgg gaatgaaaaa
     1261 ccggatatca gtatacgtgg gttaagtggc gattatacat tgattctggt cgatggacga
     1321 cgtcagagtg gtcgggaatc cagaccaaac ggcagcggcg gttttgaagc cggatttatc
     1381 cctcctgtgg aagcaattga gcgtattgaa gttatacgtg gccctatgtc ttccctgtat
     1441 ggttccgatg ctatcggagg agttattaat atcataacca aacctgttaa taaccagaca
     1501 tgggatggcg tacttgggct tgggggaatt attcaggaac atgggaaatt tggtaactca
     1561 accacgaatg acttctacct gtcaggccca ttggttaagg ataaacttgg tcttcagtta
     1621 tatggaggaa tgaactatcg caaggaagat agtatctctc agggaacacc agcaaaagat
     1681 aataagaata taacggcaac actccagttt actccgactg aaagccagaa gtttgttttt
     1741 gaatacggaa aaaataacca ggaacataca ttaacacctg gtgattctct tgatgcctgg
     1801 actatgcggg gaaatcttaa acaaccaaac agtaaaagag aaacacataa ctcacgtagt
     1861 cactgggtag cagcatggaa tgcccagggc gaaatactgc atcctgaaat tgctgtttat
     1921 caggagaaag ttattcgtga ggttaaatct ggtaaaaaag ataaatataa tcattgggat
     1981 cttaattacg agtcaagaaa accggaaata accaacacga tcatagatgc aaaagtgacg
     2041 gcatttctgc cggaaaatgt gctgaccatc ggaggtcaat ttcagcatgc agaactccgt
     2101 gatgactcag ccacaggtaa aaaaacgaca gaaacacagt ctgtttcagt taaacagaaa
     2161 gctgttttta tagaaaatga atatgctgca acggattctc tcgccctgac tggagggctg
     2221 cgcctcgata accatgaaat ctatggcagt tactggaatc caagattgta tgctgtttat
     2281 aacctgaccg ataatctcac tctcaaagga gggatcgcaa aagcatttcg ggccccttcg
     2341 attcgtgagg tcagtcctgg atttggaaca ctgacgcagg gtggtgcctc tattatgtat
     2401 ggaaacaggg acctgaaacc ggagaccagt gtaaccgaag agatcggtat tatttataat
     2461 aatgacagtg gtttttcagc aagcgcgact ctgtttaata cagattttaa aaataagctg
     2521 accagttacg atataggcac aaaagatcca gtcactgggc taaacacttt tatttatgat
     2581 aatgtgggtg aggcaaatat cagaggtgtg gagcttgcaa ctcagattcc tgtgtatgat
     2641 aaatggcgtg tatctgcaaa ctatacattc actgactccc gtcgagaaag cgatgacgaa
     2701 agtctcaatg gcaagtcgct gaaaggggaa cctctggaaa gaactcccag acatgcagcc
     2761 aatgcaaaac tggaatggga ttacactcag gatattacat tttattcatc tctgaattat
     2821 acaggaaaac aaatctgggc agcacaaaga aatggtgcca aggttccccg tgttcgtaat
     2881 ggattcactt ctatggatat tggtatgaat taccagttcc tgccaggcac gctgattaat
     2941 tttgccgtac ttaacgtcac agacagaaag agcgaagata tcgataccat tgatggtaac
     3001 tggcaggtcg atgaaggacg tcgttactgg gctaatgtaa gaatatcctt ctgagagaat
     3061 atagataacc tgaggccgtg cgcctcaggt accagtatag taatcagtaa cgtcagcttc
     3121 caggttgtaa tgcttttaat tttgataaaa ataaacttaa taaattacat ttgttaaata
     3181 ttgataattt taatttggtt ttattactcc agttatactc acactctatc ttta
//
LOCUS       DAZKFU020000364         3230 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZKFU020000364 DAZKFU020000000
VERSION     DAZKFU020000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.366"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..290)
                     /locus_tag="ACHOQQ_RS23190"
                     /old_locus_tag="pgaptmp_004638"
     CDS             complement(<1..290)
                     /locus_tag="ACHOQQ_RS23190"
                     /old_locus_tag="pgaptmp_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGU4472785.1"
                     /translation="MKRTLLIAVWAIGLMSDGAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYV"
     gene            complement(298..483)
                     /locus_tag="ACHOQQ_RS23195"
                     /old_locus_tag="pgaptmp_004639"
     CDS             complement(298..483)
                     /locus_tag="ACHOQQ_RS23195"
                     /old_locus_tag="pgaptmp_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGU4472786.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(480..3119)
                     /locus_tag="ACHOQQ_RS23200"
                     /old_locus_tag="pgaptmp_004640"
     CDS             complement(480..3119)
                     /locus_tag="ACHOQQ_RS23200"
                     /old_locus_tag="pgaptmp_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGU4472787.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVGAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQVSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
BASE COUNT          778 a          897 c          747 g          808 t
ORIGIN      
        1 acatattgcc ccgacttcgc gcgagcaaca agcttttgtc cagccagttt ggctatctct
       61 ttagtggata cattattttg ctttgccagt tgttggtaac tatggttacg agcttcatta
      121 atatctttta ctaatgcctg tgtttcggca tccgtttgta gcgccaccag gtaaccgtta
      181 agcgtttcac ctacccgccc ctgacttctg gcttcgttca gggttaaagc cattgcaccg
      241 tcgctcatca ggccaatggc ccatacagct atgagtaatg ttcttttcat catcacctca
      301 aaatagatcg ctacgggttt tgagcaggtc ttcaacgtcc ttatccgctt taatgatgat
      361 ttcatgctca attttaacgt tcatattaat ggtgattggc tctttcggcg cagccacttc
      421 aatgcgaggc gtgcaaccaa ccaacataaa agaggacgca aacgcggcta tcactatttt
      481 cattgttttt cctcacactc ttttcctgat gggcagccag tttccggtag ctgggcattc
      541 tgctccagcc atacctggag attatcgcca aagcgtaaac tgcgccataa cgtaaaaata
      601 ttctcttcat ggtgataatt aaggtttacc gtaccgcttt taccatcgac gcgactggtg
      661 cccgaaatgt tggcctgcat ggttaacaca ccgagattat ctaaattaat ccttgttgat
      721 gagcgactga tttccatata acgcaaccag ttgatggcag ttccagcggt catattgtct
      781 ttggccaccg catctgcagt gtctttatcc agacgcagcg tcatcggccc cgcgtttgcc
      841 aaccagccat ctttgacaat ccatttttca ttgttcagcc atagtggcaa cgccccacta
      901 aatgccccag acatagcaaa ctgtttcgga ttaaccgcgc taaccagttc gctggatgag
      961 agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg tttcatcagc
     1021 acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaatggttc ttcttcagtc
     1081 cacggataac gcccttgcaa atcagccgta atatttttcg ctgtcaccag attaatcact
     1141 tcggcaattc gcaacgtaac ggggccgcga gtccccagat gccaggctcc atcggcaaaa
     1201 cggaaaggca ggacaaaatc gacaccgttc acctgattat ctggcatcca ggcactaccg
     1261 cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc agaaaatgca
     1321 acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg caccagcggc
     1381 tggaatacag tcagtgattg tttaggccac caggcattac cgcgcagacg aataccgtcc
     1441 cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc tttaaagagg
     1501 aaatacgtcg gatcgcgccc atcgacgcta aattttaagg ttgatggcgg taacacactg
     1561 ccgccagtga acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa ggagggatgc
     1621 tgtgcgtcac gtacccagac gacgggcttg tcgagaatta atcgcggctt ttctaccgtc
     1681 atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt aatcgtgctg
     1741 tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt aaaactccct
     1801 tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt cgccagccca
     1861 tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat ggcctgcaaa
     1921 cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg gatttcatcg
     1981 atgtccagcg aatcgatgac tcttcccttt gaacgaagta acgcgcctgg ctcaaaggtc
     2041 agcgttgggt cagacagact tccacttacc tgcgcaggta aacgggcata taaaatgagg
     2101 tccgcctgtt tcgcttcacc ggtcagctgc agaggcagct gactgttatc catgcttaat
     2161 tttcctgggc caaaatttag caccgcgttg cctttacccg cttgcccctg ggtcagcaca
     2221 ctcagtcgtc cgctgaccag agcgttctca agccctgcct gccagttgtc gactttgaca
     2281 cccagtcggc cactcaaagg aaaacctgca tacggccagc tccagcgacc atcgcttacg
     2341 gtcaattgtt gacgcgtaat ttgccacggc aaatcgagca acggatcgcc gttatcccgt
     2401 gccagcacga tcaattgccc gctattttcc tgccagtcca gctcggcacc caccagtgac
     2461 ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag tccatccggc
     2521 acgagcggca tagtaaattc ccccaccagt ttaaccggcg gctgattttc aaacgcgacg
     2581 acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa tttaactttt
     2641 tcgccctgat aacgcagttg ctggatatca gaggttaatg cgagagagag ttttccctgc
     2701 cattcctgcc agggagaaaa aatcagttta tcgatattga tccaggtgtt aggcagcatg
     2761 gactgccact gcgcgagggt ttttggagca gctggcgatt gctccgtctg cggcaatttc
     2821 gccagacaag cagaatcaag ttctaccgtg ccgacgttca ataaccagcg gctgggatgt
     2881 gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata acggagatcg
     2941 ggaatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat acgtgtcccg
     3001 agcggtagcc agatgcccgc cagcgtggga acccacagcc cgagcgtcat cagcagcgtc
     3061 aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt acccagcatg
     3121 ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat tgtggcatgt
     3181 ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga
//
LOCUS       DAZKFU020000365         3193 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZKFU020000365 DAZKFU020000000
VERSION     DAZKFU020000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.367"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..72
                     /locus_tag="ACHOQQ_RS23205"
                     /old_locus_tag="pgaptmp_004641"
     CDS             <1..72
                     /locus_tag="ACHOQQ_RS23205"
                     /old_locus_tag="pgaptmp_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HGU4472788.1"
                     /translation="LLTYLTNLYFKIREDRRKAARGE"
     gene            76..303
                     /locus_tag="ACHOQQ_RS23210"
                     /old_locus_tag="pgaptmp_004642"
                     /pseudo
     CDS             76..303
                     /locus_tag="ACHOQQ_RS23210"
                     /old_locus_tag="pgaptmp_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            956..1243
                     /locus_tag="ACHOQQ_RS23215"
                     /old_locus_tag="pgaptmp_004643"
     CDS             956..1243
                     /locus_tag="ACHOQQ_RS23215"
                     /old_locus_tag="pgaptmp_004643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472789.1"
                     /translation="MQPNTGAHSSTFGVPENMQSVLSNADELLLIHLTKEKRSESLVC
                     LNIQQNPQGVSRSVPGVYESHNIDRDDIYEWLFYPMVVLCVPVNGGLPLDR"
     gene            1378..1911
                     /locus_tag="ACHOQQ_RS23220"
                     /old_locus_tag="pgaptmp_004644"
     CDS             1378..1911
                     /locus_tag="ACHOQQ_RS23220"
                     /old_locus_tag="pgaptmp_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4472790.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKKKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            2185..2880
                     /locus_tag="ACHOQQ_RS23225"
                     /old_locus_tag="pgaptmp_004645"
     CDS             2185..2880
                     /locus_tag="ACHOQQ_RS23225"
                     /old_locus_tag="pgaptmp_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4472791.1"
                     /translation="MKEMISVDREISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPQTLPEALRFAAKLAEQKAELENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            2975..3106
                     /locus_tag="ACHOQQ_RS23230"
                     /old_locus_tag="pgaptmp_004646"
     CDS             2975..3106
                     /locus_tag="ACHOQQ_RS23230"
                     /old_locus_tag="pgaptmp_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472792.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVALFLSITWMALTSAGLVG"
     gene            3118..>3193
                     /locus_tag="ACHOQQ_RS23235"
                     /old_locus_tag="pgaptmp_004647"
     CDS             3118..>3193
                     /locus_tag="ACHOQQ_RS23235"
                     /old_locus_tag="pgaptmp_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4472793.1"
                     /translation="MLSAFTVILLVVCGALSLGLNHYRD"
BASE COUNT          873 a          659 c          848 g          813 t
ORIGIN      
        1 ctgctgacat atctgacgaa cctgtatttc aaaatcagag aggaccgtcg taaggcggcg
       61 cggggagagt agacgatgaa taaaaaatat gaactggttg taaaagggat aaatcattac
      121 tcggataaga taactgttac tgtgacactg gaaattgccg ggcagccgtc gttgttgtcg
      181 ccatatgtgg cgattagtct tgaccgtact gaaggtgcca cgctggaatt ttacgaagct
      241 gaggcgaaaa aacaggcgaa gcagtttttc atggatattg ctgccgcttt atgtgaaggg
      301 gatgaacagt cacaggaaaa gcgcctctgt tcagaagagc ggtataccat acaaattaac
      361 aatgcatata acacgataca gtcagaaaaa gatgatatcg aatcacgcat tgaaaaactg
      421 gagaacagcg tcgttgaact gaataaaaaa ctgtcggtgc agatcccttc cgaaggtgaa
      481 aaaaaacgcc gtgatgagca gttcgcggcg ttttacgatt attgccggaa agtgatgagc
      541 agaaatctcg cagagtgttt ccgtattcaa aaaaacagag agcgctctgc aaatagtgag
      601 attctggata attttttaaa ggggacgcat ctcgccagtg attgtttcat tgctcattct
      661 tttggtggct ttcatgaggc catcaaacat gatgttgaga gaagtccagg tatcctctgt
      721 tccattcccg taagcatcga aatagacaca aatacgataa ccggtgaact cgttaccgcc
      781 ggagtcggta tattccgtca tgaggggaac gatgccaaag gttcctttac aaaaatcggg
      841 ccgttctttc tgaaggtaga gcgatagttt gttaagtacc catccgacag ctctctcatt
      901 ttggtttatt ttcgtatcgc gaaacaaaaa ttcgaagcgg ccagatgacg catgggtgca
      961 accaaatacc ggggcgcatt catcaacctt tggtgttcct gaaaacatgc agtccgtgct
     1021 ctcgaatgca gatgaattgc tgttgatcca cttaacaaaa gaaaaaaggt ctgaatcgct
     1081 tgtctgtctg aatattcaac aaaatcctca aggtgtgtct cgatcagttc cgggtgttta
     1141 cgaaagtcat aatattgacc gggacgatat ttatgagtgg ttgttttatc ccatggttgt
     1201 gctctgcgtc cctgtaaacg gaggtctgcc gctggataga taagcataag taatctcctg
     1261 gtggaatgta atccacgatc atccggggaa attaagaacc cgccagtgcc caccactggt
     1321 ggggtgaagg cttaacatat ccgggtattc ggaaccgata aatcctgata aatatccatg
     1381 aacgcaaaaa tcagatacgg cctgtcggct gccgttctgg cgctgattgc cgcaggggcg
     1441 cctgcgcctg atattctcga ccagtttctg gatgaaaaag aaggtaacca caccacggca
     1501 taccgtgatg gtgcgggtat ctggaccatt tgtcgtggtg ccaccagggt ggatggcaaa
     1561 cctgtcgtcc cgggcatgaa gttgtcgaag aaaaaatgcg accaggttaa cgccattgag
     1621 cgtgataagg cgctggcatg ggtggagaaa aacatccggg tgccactgac cgaaccccag
     1681 aaagcgggga tcgcgtcatt ctgcccgtat aacattggtc ccggtaagtg tttcccgtcg
     1741 acgttttata aacgaattaa tgcaggcgat cgcaggggag cgtgtgaggc gattcgctgg
     1801 tggattaagg acggtggcag agactgccgt attcgctcaa ataattgcta cggtcagata
     1861 tcccgtcgtg accaggagag tgcgctggcg tgctggggaa tcgacagata agcagaatat
     1921 tttgctgaaa aatgacgttg gtcaacgcgg gtggataaca cgaaatcctg cgaactggca
     1981 aaacctaagt gaataaagtc aggaacattg tttcacgcag aggcaccgta atggtgcctt
     2041 tgtcatttct gcgcttcgca caagcgtaaa taaaccaaag aacctttcag gatgagccct
     2101 ggtggataac cggcagtggt ctggttaacc ctctttgggc tggttattcc tgtgcgcagg
     2161 gttcatcact aaaaggaatc aaccatgaaa gagatgattt ctgtcgatcg tgaaatatcc
     2221 atgagtagtc tggattttct gaataacatt attaatccag cccgggcaga agccggagaa
     2281 gtccctcatg aaccgcgtaa gtttcttgca aaaattgagg atgagctaga gcttgatgga
     2341 accggaaaaa aattccggtt aaacaataac cagacaagaa cggcatacta tgatctggat
     2401 tttgaccaga tgatgctcgt tggcatgaga gagtcaaagg ccgttcgtcg ttctgtgctg
     2461 gcaaggctca aagcgatgca tggtattcag atcccccaga ctttacctga ggcgttgcga
     2521 tttgcggcaa aactggcaga acagaaagca gagctggaaa atcaactggc aatagcagca
     2581 ccgaaggctg aatttgttga taactatgtt gaagcatctg gtctgatggg attccgggaa
     2641 gttgctaagt tactcggtat caaagaaacc gatttccggc tgtttttgtt ggagaacgga
     2701 ataatgtatc gcctcgctgg aaaaatgacg ccttactcgc atcacctgga tgcggggcgg
     2761 tttagcgtga aaacgggcga ggcgggcaat ggtcatgctt ttacgcaggt taaattcacc
     2821 ccaaaaggtg ttcagtggat tgctggtctg cttgctgcat ggagagctac cgcagcatga
     2881 agatgataaa aatggactgg aaatttttgc tggtctggct gattccgttt ttatgggtgg
     2941 ttgcccggtt aattactgct attaagaggt aaagatgtca gacaaactca taatgctggc
     3001 gaagggcctt tgtgtaatcg tcggtatttc attttcacta atgctggttg ctctttttct
     3061 ttccataacc tggatggcgt tgacttcggc agggctggtg gggtgagcat aaaccgaatg
     3121 ctttccgcgt ttaccgttat tctgctggtg gtctgtggtg cgcttagtct ggggctgaat
     3181 cattaccgcg ata
//
LOCUS       DAZKFU020000366         3184 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZKFU020000366 DAZKFU020000000
VERSION     DAZKFU020000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.368"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            166..1686
                     /locus_tag="ACHOQQ_RS23240"
                     /old_locus_tag="pgaptmp_004648"
     CDS             166..1686
                     /locus_tag="ACHOQQ_RS23240"
                     /old_locus_tag="pgaptmp_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGU4472794.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIANHL
                     QAVCAFLEGKHTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSNIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1711..2049)
                     /gene="maoP"
                     /locus_tag="ACHOQQ_RS23245"
                     /old_locus_tag="pgaptmp_004649"
     CDS             complement(1711..2049)
                     /gene="maoP"
                     /locus_tag="ACHOQQ_RS23245"
                     /old_locus_tag="pgaptmp_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGU4472795.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2168..3007
                     /gene="hdfR"
                     /locus_tag="ACHOQQ_RS23250"
                     /old_locus_tag="pgaptmp_004650"
     CDS             2168..3007
                     /gene="hdfR"
                     /locus_tag="ACHOQQ_RS23250"
                     /old_locus_tag="pgaptmp_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGU4472796.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3103..3178)
                     /locus_tag="ACHOQQ_RS23255"
                     /old_locus_tag="pgaptmp_004651"
     tRNA            complement(3103..3178)
                     /locus_tag="ACHOQQ_RS23255"
                     /old_locus_tag="pgaptmp_004651"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3143..3145),aa:Trp,seq:cca)
BASE COUNT          861 a          779 c          776 g          768 t
ORIGIN      
        1 tcgtagttca tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact
       61 caacttcact tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta
      121 cagcagcgat atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt
      181 gttcataccc gcgcagccct gggagtaaat gcgcccccaa tcactgttga ggtacatatc
      241 agtaaaggtc tacccggctt aacgatggtg ggcttaccag aaacaacggt aaaagaagcc
      301 cgcgatcgcg tgcgcagcgc cattatcaat agcggatatg aatatccggc caaaaaaatc
      361 accatcaacc tagctccagc cgatctgcca aaagaagggg gacgatatga tttacctatc
      421 gccattgcgt tgctggcggc ctcagaacag cttacagcca ataagttaga tgaatatgaa
      481 ttagtcggag aactggcgct tacaggcgct ctgcgtggcg ttcccggtgc aatctccagt
      541 gcaactgaag ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa
      601 gtggggctaa ttaacggtga aggatgcctg atagccaatc atctgcaggc tgtctgtgcg
      661 tttctggaag gtaagcacac tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg
      721 ctacaacatg atctcagtga tgttgtcggt caggagcaag gaaagcgagg actggaaatt
      781 accgccgctg gcgggcacaa ccttttactg atcggaccgc cgggaacagg taaaacaatg
      841 ctcgccagcc gtattaatgg tcttttgcca gatttaagca atgaagaggc actggagagc
      901 gctgcgatat taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgcccg
      961 ttccgctcac ctcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca
     1021 gggcctggtg aaatttcgtt ggcacataac ggcgtgcttt ttcttgatga gctacctgaa
     1081 tttgaacggc gtacactgga tgctttgcga gagccgattg aatccgggca gatccatctt
     1141 tcacgcacac gagcaaaaat aacctatcca gcccgtttcc agcttgttgc ggcgatgaat
     1201 cccagcccta ccggacatta tcagggaaac cataaccgct gcacgccaga acagacatta
     1261 cgttatctca accggctctc ggggcccttt ctcgaccgct tcgatctctc actggagatc
     1321 ccattaccac cccccggcat tttgagtaaa acggtagtgc cgggagaaag cagcaccacc
     1381 gttaaacaac gtgtaatggc tgccagagag cgccaattta agcggcagaa taaactgaat
     1441 gcctggctgg atagtccgga aatacgcaaa ttctgcaagc ttgagagcga agatgcgcag
     1501 tggctggaag aaacgctgat ccatctgggg ttatcgattc gtgcctggca gcggttattg
     1561 aaagttgcac gaaccattgc tgatattgat cagtctaaca ttatcacacg tcagcatttg
     1621 caggaggcag ttagctatcg tgcgattgac cgtttgctca tccatctgca aaaactactg
     1681 acataaaaaa agggcatttc gcccttttta ttaatcgtca gaatcggtgt agtcttcagc
     1741 accttcaacc tgcggtttac cgccggaaag ggtgtgaaaa cgttttggac gcttaatacg
     1801 cgtcatatac ttggaccaca cgcgttctgc ttctgtcact ggctcacgtt cgccacggca
     1861 tactgctacg aagagtttct cttcctcggt aaccggctcg cgtttgccaa gatccaactc
     1921 attgaaggca taaccatgac gctcaagcag ttgtgcctct ttgatggtga aatcaccatg
     1981 acgagagaat ccacgtggat aatgtttatt gtcgaaatat cgattagtcg tcgtaaagct
     2041 ttccgccatc ctgcacgctc ctaattcttt gaccgagcta gttatggcgc ggagtattag
     2101 ttacgcttga cagagtgtaa aacaaaacat ttaaatcata acgacaaata attttgcgga
     2161 gagcactgtg gatacggaat tgttaaaaac tttcctggaa gttagccgaa cgcgtcactt
     2221 tggtcgagcg gctgaatcgc tctatctgac ccagtcagca gtgagctttc gaatcagaca
     2281 actggaaaat caactgggtg tgaacctttt cacccgccac agaaacaata tccgtttaac
     2341 cgctgccggt gaaaaactac tgccttatgc agaaacgctc atgagcacct ggcaggccgc
     2401 ccgtaaggag gtggcgcata cctcacgaca taacgagttt tctatcggtg ccagcgcctc
     2461 attgtgggaa tgtatgctta atcagtggct gggacgtttg tatcaaaatc aggatgccca
     2521 tacaggctta cagttcgaag cgcgaattgc ccaacggcag tctctggtaa aacagctgca
     2581 tgaacgccag cttgatcttc ttattaccac tgaagcgccc aaaatggacg aatttagtag
     2641 tcagttgctg gggtatttca ctttagcgct ttataccagt gccccttcca aactaaaggg
     2701 agatcttaac tatctgcgac tggagtgggg gccagatttt caacagcatg aggcaggttt
     2761 gatcggtgct gacgaagttc ccattctgac aaccagttct gctgaactgg cacagcaaca
     2821 gattgcgatg cttaatggtt gcacctggct acccgtcagc tgggcgcgta aaaaaggcgg
     2881 cctacacacc gttgtcgata gcacaacact ttcgcggccg ctttatgcca tatggctgca
     2941 aaatagcgat aaaaatacgc tgattcgcga tctattgaaa attaacgtgc tggatgaagt
     3001 gtattaatct gaatggctgg caaggatgcc ggtagaagga tttacttcag agagggttat
     3061 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggcaggg gcggagagac
     3121 tcgaactccc aacacccggt tttggagacc ggtgctctac caattgaact acgcccctaa
     3181 ttag
//
LOCUS       DAZKFU020000367         3171 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZKFU020000367 DAZKFU020000000
VERSION     DAZKFU020000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.369"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..325
                     /locus_tag="ACHOQQ_RS23260"
                     /old_locus_tag="pgaptmp_004652"
     CDS             <1..325
                     /locus_tag="ACHOQQ_RS23260"
                     /old_locus_tag="pgaptmp_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4472797.1"
                     /translation="ITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWL
                     LTNTELSQAEISWRVGYENVDHFGKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQP
                     VSLAG"
     gene            complement(368..1519)
                     /locus_tag="ACHOQQ_RS23265"
                     /old_locus_tag="pgaptmp_004653"
     CDS             complement(368..1519)
                     /locus_tag="ACHOQQ_RS23265"
                     /old_locus_tag="pgaptmp_004653"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGU4472798.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPEPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1533..3128)
                     /locus_tag="ACHOQQ_RS23270"
                     /old_locus_tag="pgaptmp_004654"
     CDS             complement(1533..3128)
                     /locus_tag="ACHOQQ_RS23270"
                     /old_locus_tag="pgaptmp_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGU4472799.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT          777 a          804 c          730 g          860 t
ORIGIN      
        1 aatcacttta gaacagttat cgaaaaaatt tcgtgccagc gtcagttata tttgccatga
       61 gtttaccaaa gagtatcgta tttcccctat taactatgtt attcaacggc gtatgacgga
      121 agcgaaatgg ttactcacta atactgaatt atcacaggca gagatctcct ggcgtgtggg
      181 ttatgaaaat gtcgatcact ttggcaaact gtttttgcgc catgtcggct gttcgcccag
      241 cgattaccgc aggcaattta aaaactgttt tgcggaacaa gaaatcctat ctgaatttcc
      301 tcaaccggta agtcttgccg gataaatctg gcggcgcagt ttgttaagaa gctgcgccgc
      361 ctgcagatta tttgttctga tagtctttca ggatctgccg acctgctacg taaatcataa
      421 tttcgtctgt accgccgccg atacgttcac aacggacatc acgccagaag cgggagacgc
      481 gcgcttcatc ggtatagccc agaccgccca tgatttgaat cgcatcatca atgacttcca
      541 ttgcggtacg tgcgcaatac agttttgcaa gcgccgcgct ggtgcgcagt gactgatgct
      601 gatcggcttg ccatgccact ttcagcacca tgttgcgcat gttgtcaatc ttaatcgcca
      661 tcagcgccag tttttcctgg atcatctggt tatgaccaat gggcttacca aaagcgatac
      721 gttggttggc atagcgggcg gcatcttcaa aggcgcattc ggcaaagccg gtgctgcgcg
      781 cggcgttgat caggcgctcc atctcaaagt tgtacatcac attgaggaaa cccattcctt
      841 cttcgcccac catgtcgctc tcttcaactt ccacgttgtc gagatagact tcgcaggtgc
      901 tgagcatatg ccagccgatt ttatgcagcg ggttaatctt aatgccgggc ttactggagt
      961 cgacccacca cagggtgaag gcttttttgg gatctttcgg ttcaggatcg cgcgccagca
     1021 ccagcatata cgggtactct ttcgcaccgg taataaaggt tttctgtccg ttgatgtaaa
     1081 ccttgccgtt tttacgcgtg taagtggtag tggcactgtt gttgtctgag cctgcgcctg
     1141 gctccgtcaa cgccagggca taggcgggat cacccgtttc cagtgtgctt tctgccgttt
     1201 tacgtagctg ctctgcagaa ccgaaacggc gcatactgtg aatacattga ccgttggtaa
     1261 tcaaaaatgc cggagcaccg cattttgaca cttccatcag tgccagcatt tgggtgacgt
     1321 aatccgcagg tataccacca aattcttccg gcacgccaag catggaaata ccgttatccg
     1381 ccagtgcccg cataaattca cgcgggtatg tcccgttttg atcgcaggtg cggaaatact
     1441 cttccggaaa gttagtcgta atcagttcgc gaatactggc cagcagcagt tcttgttctt
     1501 cagttaaaga aaaatccatc gtgatactcc tgttaatgag ccgcttcagg caggacaaaa
     1561 cccatcgccg catcgataaa taatctttcg tccatcagtt tgagttctgg cgaaatcact
     1621 ggggtgaaat ccattttgtc gagaatatct ttttgtaaat cgacgccagg ggcgatttcg
     1681 attaaatgca ggccgtcttc tttcagcgtg aatactgcgc gctcagtgat ataacgaaca
     1741 tccagccctc gctcgagagc gatttttccg ctgaaagtaa tttccggtag ttcccgaata
     1801 aatttcttca cccgtccttc ctggacgata tttaatttgc cgtcggtaat ttctgttttt
     1861 aaactgcccg cagttaatgt gccgcagaaa atgattttct tcgaagtggc actgatatca
     1921 ataaatccac cggtacccat gattttgcca ttgaatttat gcacgccgac gttaccgtgc
     1981 tggtcgactt cagcaaaact caaataacaa acatccagac caccaccgtg ataaaaatca
     2041 aactgggacg tcatatccag aatggcgcgg gtattcacgt tcgcgccaaa ggcgataccc
     2101 tgtgaagtaa ttccgccaat cggacctgtt tctaccgtca gaataaagtc atcagcacaa
     2161 ccttcttccc gggcgaccag gccaatgccg tcagcaatac cgacgccgac attccccacc
     2221 gcgcctttac gcatttcgaa taatgcgcgc cgcgcaacta atttacgttg gtttaggggc
     2281 aggctaagtt tggtactgtc atcaagtgtg aagtcaccag aaataaagcg gttaaccggt
     2341 gcaccgccat acagttgggt ttgatccgga tcgaccacca caatatccac cagataaccc
     2401 ggaatacgga cagatttagg atgcagcgtg gctttcttaa ccattttctg cacctgcatc
     2461 atcacaatac cgccgttatt gtgcaccgct tgggcaataa ccaatgcgtc gagatacatc
     2521 acctcatctt caaaagtggc gtagccttca ctgtcgcagg tggtagcgcg aatgaaagca
     2581 atatctggcg caatcgcttt gtaatagaga tattctttgt tatcaaactc gaccagttta
     2641 atcaggtctt ctttagtgac ttcattcagt ttgccgcctt gctggcgtgg atcgacaaat
     2701 gtcccgatgc caatatcact aataatacca ggctggtggg ctgcggcggc gcgtaaggtt
     2761 tgtgtaagta caccttgtgg gtagttataa gcaataattt tattttgttc tgcgagttca
     2821 gaaatacgcg gcgattgtcc ccagtgacca cataatgccc atttcaccag accttcttgc
     2881 gccagaggac taataccacg gtcggcgcga tcgccaagcc ctgttggact aataatcgat
     2941 aaattacgtg gcgtttgagt ctgtttatat ttatcagcaa gagcagtaat taacgtggtg
     3001 gcttccagaa taccgccgcc agcgcctaac acacaaagtg ttgcttcgtc gggaatataa
     3061 ttcaccgctt cctgtgccga caggaccggc actcgtccat taatacgagg tggttttaca
     3121 ggtttcattg ttaattcctg attcaggtgt gattttccct acgccagatg g
//
LOCUS       DAZKFU020000368         3165 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZKFU020000368 DAZKFU020000000
VERSION     DAZKFU020000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.370"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(54..815)
                     /gene="kduD"
                     /locus_tag="ACHOQQ_RS23275"
                     /old_locus_tag="pgaptmp_004655"
     CDS             complement(54..815)
                     /gene="kduD"
                     /locus_tag="ACHOQQ_RS23275"
                     /old_locus_tag="pgaptmp_004655"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGU4472800.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(845..1681)
                     /gene="kduI"
                     /locus_tag="ACHOQQ_RS23280"
                     /old_locus_tag="pgaptmp_004656"
     CDS             complement(845..1681)
                     /gene="kduI"
                     /locus_tag="ACHOQQ_RS23280"
                     /old_locus_tag="pgaptmp_004656"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGU4472801.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVTDEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASDDTATPAKFYYNCVPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1968..3149)
                     /gene="yqeF"
                     /locus_tag="ACHOQQ_RS23285"
                     /old_locus_tag="pgaptmp_004657"
     CDS             complement(1968..3149)
                     /gene="yqeF"
                     /locus_tag="ACHOQQ_RS23285"
                     /old_locus_tag="pgaptmp_004657"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGU4472802.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVIDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          830 a          875 c          715 g          745 t
ORIGIN      
        1 caggtataaa aaaccctgcc atgcggcagg gtcataaaag taagagaaat gaattaacgc
       61 gccagccaac cgccatccac ggcaatggta taaccattca cataatctga agcgctggag
      121 gcaaggaaca ctaccggccc catcaggtca ctcggcagtc cccaacggcc agctggaatg
      181 cggtcgagaa tttccgcgct acgttgttca tctgcccgta gttgttgagt attgttggtc
      241 gccatgtaac ccggagctat cgcattaaca ttaatgttgt gtttagccca ttcgttcgcc
      301 atcaatcgcg tcacgcccat aacaccgctt tttgatgcgg tataagaagg cacacggatc
      361 ccgccctgga aggagagcat tgacgcgata ttgataatct tgccgccatt gccttgcgcg
      421 ataaagtgtt tcgccgctgc ctgagacatg aagaatacgc tcttgatatt caggttcatg
      481 acatcgtccc agtctttttc gctgaactcg agagcatctt cgcggcgaat caatccggcg
      541 ttattcacca ggatatcaat atgaccaaac tcagctaccg cgcgatccag cagtgctgga
      601 ataccatcaa tctttcgcag atcggcggtc aggcttaaaa aacgacgccc cagcgccgtg
      661 acctgcttga tggtttcagt cggttcaacg atgttaatgc caacaatgtc acagcccgct
      721 tgcgccagcc ccaacgccat cccctggccc agtccagtat cacaaccagt gacgaccgca
      781 actttacctt cgagagaaaa tgcacttaaa atcataacaa taccttattc gtttatgccg
      841 acaactagcg caaatcttta acggccacat ggtccatatc atcaaagacc tggttttcac
      901 cgaccatgcc ccagataaag gtataagctt tggttccgac gccggaatgg atcgaccagc
      961 tcggggagat caccgcctgc tcgttatgca tcacaatgtg acgcgtttct tgcggctgcc
     1021 ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa atagaaataa acttccatcc
     1081 ggcgctcgtg ggtgtgacac ggcatggtgt tccacaagtt acctggagcc agctccgtca
     1141 gccccatact caattggcag gtttccagca catccgggac aaaatatttg ttaatcgtgc
     1201 gacggttact ggtgaggtta tcccctaacg tgactggaga tacttcgtcc ggtgtgactt
     1261 ttttggtggg atacgtcgta tgcgcgggta cgcaattgta ataaaacttc gccggagtgg
     1321 cggtatcatc actggcaaag acaacttctt ttgcaccttt acccacatac agggcgtcgc
     1381 ggtgaccgat ttcatagcat tgaccatcga cagtaatcgt accggcaccg ccaatattga
     1441 taacacctaa ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta ccaacttccc
     1501 cgccaacgga aaccgtttta gttaccggca taatgccgcc aacaataata cggtcaatgt
     1561 ggctgtaaac catggtgtac tcatcggtga caaatacctt ttcaaccaaa aattcattgc
     1621 gcagcccctg ggtatccagt gtttttgcgt gcgcactgtg gatgctctgt cttacgtcca
     1681 catcaacctc cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc
     1741 gttttctcta taaggggatg atatgcccat gggatagggt tttcaattaa aaataaaaca
     1801 atgtttcatt tttaagttaa ggattaaaaa aagtgccgtg tcgatcacga atatgtcatg
     1861 agaatggaaa aatcagggag cccagaaaat gtatgtcgac ttgccagcca tcattattca
     1921 acagattaat gacggcagat atttatcggt aaggaggaaa tgcagagcta ctcgtcacgt
     1981 tcaatggtca atgctacgcc ctggcccccg ccgatacaaa gcgttgccag tcctttgcgg
     2041 gcattacgtt tcaccatttc atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct
     2101 atcgggtgac cgagtgcgat cgcgccacca ttgacattga cccgacgctc atcccattca
     2161 agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt cattagcctc gataagatcg
     2221 acatcagcca actgccagcc cacacgctcc aggcaacggc gggtcgcata caccggcgca
     2281 attcccatca atgctggatc tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg
     2341 ggtaaattca acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg
     2401 tttatggatg atgcattacc cgctgtcaca gaaccgagac tatcaaatga aggatttaaa
     2461 cgggctaagc cttctgcact ggcgtcagtg cgtggctgtt catcggtatc aataaccaac
     2521 gtctgaccat tactttgggt cattaccggg acgatctcat ctttaaatcg tccggcgtca
     2581 atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg
     2641 atgccatatt cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca
     2701 tcccacaacc catcatgcac cagactgtca accaactggc tattgccaag ctgtgcaccg
     2761 gtgcggctat cagtcagaac atgtggtgcg cggctcatgt tttcctggcc accggcgatg
     2821 acaatatcag cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc cttaagcccg
     2881 gaaccgcaaa cgtcattaat cgtgattgca gaaacgctat taggaagacc acctttaata
     2941 gccgattgcc ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact
     3001 tcatccaccg catatgcagg aacgccggta cgttctatta acgctttcac gaccagacta
     3061 ccaagttcca cggcggaatg acccgctaac gcaccacgaa agcagccgat aggtgtccgt
     3121 aacgccccga caatcacaac gtctttcatc acaacctcgc accaa
//
LOCUS       DAZKFU020000369         3159 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZKFU020000369 DAZKFU020000000
VERSION     DAZKFU020000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.371"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1017
                     /locus_tag="ACHOQQ_RS23290"
                     /old_locus_tag="pgaptmp_004658"
     CDS             <1..1017
                     /locus_tag="ACHOQQ_RS23290"
                     /old_locus_tag="pgaptmp_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4472803.1"
                     /translation="ADCLEFIQTLPENSVDLIVTDPPYFKVKPEGWDNQWTGDEDYLK
                     WLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNHIIWAKPSGRWNGCN
                     KESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMAPLIAYFRDARAALG
                     ITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVAEEKHQRGVLEKPHH
                     QLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPVQYYPGKHPCEKPAE
                     MLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELETERFEQTVREVQDLA
                     SQNG"
     gene            1058..1133
                     /locus_tag="ACHOQQ_RS23295"
                     /old_locus_tag="pgaptmp_004659"
     tRNA            1058..1133
                     /locus_tag="ACHOQQ_RS23295"
                     /old_locus_tag="pgaptmp_004659"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1091..1093,aa:Ile,seq:cat)
     gene            1143..1219
                     /locus_tag="ACHOQQ_RS23300"
                     /old_locus_tag="pgaptmp_004660"
     tRNA            1143..1219
                     /locus_tag="ACHOQQ_RS23300"
                     /old_locus_tag="pgaptmp_004660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Arg,seq:tcg)
     gene            1233..1309
                     /locus_tag="ACHOQQ_RS23305"
                     /old_locus_tag="pgaptmp_004661"
     tRNA            1233..1309
                     /locus_tag="ACHOQQ_RS23305"
                     /old_locus_tag="pgaptmp_004661"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1267..1269,aa:Arg,seq:tct)
     gene            1399..1623
                     /locus_tag="ACHOQQ_RS23310"
                     /old_locus_tag="pgaptmp_004662"
     CDS             1399..1623
                     /locus_tag="ACHOQQ_RS23310"
                     /old_locus_tag="pgaptmp_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472804.1"
                     /translation="MKKLMVTIGPFETEVSFRVVQGESVLVEDVFHGKSTGPYVKEYL
                     IDATDENIEVVYDSVNHPDLIIKAKLKPLY"
     gene            1676..1885
                     /locus_tag="ACHOQQ_RS23315"
                     /old_locus_tag="pgaptmp_004663"
     CDS             1676..1885
                     /locus_tag="ACHOQQ_RS23315"
                     /old_locus_tag="pgaptmp_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472805.1"
                     /translation="MFVSEVLMEDKDNKGWVKGWAVVRSSPWHLVGVFATEEDAETEA
                     RKMGDKYEVHYGSHRTGSDDFVWGE"
     gene            2075..2506
                     /locus_tag="ACHOQQ_RS23320"
                     /old_locus_tag="pgaptmp_004664"
     CDS             2075..2506
                     /locus_tag="ACHOQQ_RS23320"
                     /old_locus_tag="pgaptmp_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4472806.1"
                     /translation="MFGIFKKKTRRAATEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATITGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            2503..2667
                     /locus_tag="ACHOQQ_RS23325"
                     /old_locus_tag="pgaptmp_004665"
     CDS             2503..2667
                     /locus_tag="ACHOQQ_RS23325"
                     /old_locus_tag="pgaptmp_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4472807.1"
                     /translation="MTVKLRLAAVALLLFLVVMVDFTSRIMSVLADGVLVVGIVVVLF
                     PLVKKDMPGS"
     gene            complement(2969..>3159)
                     /locus_tag="ACHOQQ_RS23330"
                     /old_locus_tag="pgaptmp_004666"
     CDS             complement(2969..>3159)
                     /locus_tag="ACHOQQ_RS23330"
                     /old_locus_tag="pgaptmp_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4472808.1"
                     /translation="IVLNNKVPPVTIYNVVPFNKRFWDLIKNSQECPANSDNVLKECF
                     NNHCILQICPYGLKQQCP"
BASE COUNT          792 a          664 c          908 g          795 t
ORIGIN      
        1 gctgattgcc tggaatttat ccagacctta ccggaaaact ctgtcgatct gatagtcaca
       61 gacccgccat actttaaagt gaagcccgag ggctgggata accagtggac gggggatgag
      121 gattacctga aatggctgga ccagtgtctt gcgcagttct ggcgggtgct gaaacctgcc
      181 ggaagtcttt acctgttctg tggtcatcgc ctggcgtctg acattgaaat catgatgcgt
      241 gaacgcttca gtgtgctgaa ccatatcatc tgggcaaagc cgtccggacg ctggaacggg
      301 tgcaacaagg aaagcctgag ggcgtatttc cccgccacag agcgcattct gttcgcggaa
      361 cattatcagg ggccgtatcg tccgaaagat gccgggtatg aggcgaaggg cagggcactg
      421 aaacagcatg tgatggctcc gctgattgct tactttcgtg atgcgcgtgc tgccctgggg
      481 ataacggcaa aacagatagt ggatgccaca ggaaagaaaa acatggtgtc gcactggttc
      541 agtgccagtc agtggcagct accgaacgaa agcgattatc tgaaattaca gtcgctgttt
      601 gcccgggtgg cagaagagaa gcatcagcgc ggtgtactgg aaaagcccca ccaccagctg
      661 ctggagacgt atacttcact gaaccggcag tatgcggaac tgcagagtga atataagcat
      721 ctgcggcggt attttggcgt gacggcgcag gtgccgtaca ccgatgtgtg gacgcataaa
      781 ccggtgcagt actatcccgg gaaacatccg tgcgaaaaac cggcagaaat gctgcagcag
      841 ataatcagcg cgagcagtcg tccgggggac ctggttgcag attttttcat ggggtcgggt
      901 tcgacagtca aagccgcgat ggcactgggg cgtcgtgcaa ctggtgttga gctggagact
      961 gaacgttttg agcagacggt cagggaagtt caggatttag ccagtcagaa cggatgatat
     1021 tgcaggatta gttacgtacc gttattatcc tgcacccggc cctttagctc agtggtgaga
     1081 gcgagcgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca ccatatcaca
     1141 taccgccatt agctcatcgg gacagagcgt cagccttcga agctggttgc gcggggttcg
     1201 agtcctcgat ggcggtccat tatcggtatt cagcgttgtt agctcagccg gacagagcaa
     1261 ttgccttcta agcaatcggt cactggttcg aatccagtac aacgcgccat acttattttt
     1321 ctggctcgct tttgcgggcc ttttttgtat ctgcgttaca ccattaacta ataaatcgag
     1381 tgcttatcag gaggctatgt gaaaaaactg atggtgacga ttggtccgtt cgaaacagaa
     1441 gttagttttc gtgttgttca gggggagagt gtacttgttg aagatgtatt tcatggaaaa
     1501 tcaacaggtc cttatgtaaa agaatatctt atcgacgcca cggatgaaaa tattgaggtg
     1561 gtgtacgatt ccgtcaatca ccctgatttg atcattaagg caaaattgaa gccactttat
     1621 tgatctgacc gggagcaatc ataaaatatc tctgggtacc cacaaggaga taaatatgtt
     1681 tgtttctgaa gtgttaatgg aagataagga caacaaagga tgggttaaag gttgggctgt
     1741 ggtaagaagc tcgccctggc atcttgttgg ggtttttgcg acagaggaag acgcagaaac
     1801 ggaagcaaga aagatgggag ataagtacga ggttcactat ggctcgcatc gaacaggaag
     1861 tgatgatttt gtctgggggg agtaacagtc gtttaacccc agaataatcc cgtaactgag
     1921 gtcgctattg gcggcctttt ttgtatccgc gccacgcccg gcgcatatca accacagagc
     1981 ctttcggggg tgagcttacg gagtggtcag tgtgactttc tctgtgggca gatcgctccc
     2041 gggcgttggc tcacccaccc aaaggaacgt cacgatgttt ggtattttca aaaagaaaac
     2101 ccgcagagca gcaacggaaa ttaaaaagtt tgagaaacgc gatctggcac aggcggttat
     2161 taatgctgcc tacctggtag cctatgcaga tggtgaatgt gaggcttcag agaaagcgaa
     2221 gatcgaacag gtattacgta accagcctgc gttgtccgcg tttacgtcag aaattaatgc
     2281 catcagtgcc acgatcacag gtcagcttga caccaacttt aaaattggtc gtcgagcggc
     2341 gttgcgtgaa attgaagatg tgaaacacga tacgcgtgaa gcggaagatg tgctggatgt
     2401 ggcggtggct attgctgaag cagatggtga aattgagccg gaagagcgta aggttctgga
     2461 agagattgct ggtgttcttg gcctgcgact ggagaaccac ctgtgacggt aaaactgcgt
     2521 ctggccgctg tggcactcct gctgtttctg gtggtgatgg tggatttcac cagcaggatc
     2581 atgtcggtgc tggcggatgg agtgctggtg gtcggtattg tggtggtgct ttttcctttg
     2641 gtgaaaaagg acatgccagg tagttagccg ggtatcagtc atgccccgaa aattttaaat
     2701 gtctcacaat tcagacggtt gacagttgtc tgttttgcgg ggagtttgtt aaaagaaact
     2761 ggcatggtga atccccctga gcggaggggc gactggtgca ttaggtgtta tctttgatac
     2821 ccaggaaagt gaacgcgcgg gtttggtggc accgggctga actcaccggg aggcacccgg
     2881 catcatgcgc atgatgatac agatacgcgg ctttagcccc tctccggagg ggcttttata
     2941 tgaattttgt aagatatgag taaactaatt atggacactg ttgttttagt ccgtaagggc
     3001 atatttgcag aatgcaatga ttattaaagc attctttcaa tacgttatct gaatttgcag
     3061 gacattcctg gctgtttttg attaaatccc agaatctttt attgaatggt acaacgttgt
     3121 aaatggttac aggtggcact ttgttattaa gtacgatac
//
LOCUS       DAZKFU020000370         3125 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZKFU020000370 DAZKFU020000000
VERSION     DAZKFU020000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.372"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..2550
                     /locus_tag="ACHOQQ_RS23335"
                     /old_locus_tag="pgaptmp_004667"
                     /pseudo
     CDS             71..2550
                     /locus_tag="ACHOQQ_RS23335"
                     /old_locus_tag="pgaptmp_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            2564..>3125
                     /locus_tag="ACHOQQ_RS23340"
                     /old_locus_tag="pgaptmp_004668"
     CDS             2564..>3125
                     /locus_tag="ACHOQQ_RS23340"
                     /old_locus_tag="pgaptmp_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HGU4472809.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYGELSAQHNLVVQGDFALTQNQ
                     TATHTYTFNDTSCVSTNTVAQIDPSDIVIGLYNDTIKLKLHFEWEKKGSITLSNNQTS
                     FTSGYNVTVTSAASDAIVNLSGGGSGSLMINSVATLSSASSSTRLAAFLQFAGCFLGG
                     TSWDICVQNYKNALSKNAGVYSFNLAL"
BASE COUNT          859 a          587 c          796 g          883 t
ORIGIN      
        1 ctggcatctg accattatcg atgaccatgg caactatatt agtgacaaaa tttaattgca
       61 ggagctgcct atgttgagaa tgaccccact tgcatcagca atcgtagcgt tattgctcgg
      121 cattgaagct tatgcagctg aagaaacctt tgatacccat tttatgatag gtggaatgaa
      181 agaccagcag gttgcaaata ttcgtcttga tgataatcaa cccttgccgg ggcagtatga
      241 catcgatatt tatgtcaata agcaatggcg cgggaaatat gagatcattg ttaaagacaa
      301 cccgcaagaa acatgtttat caagggaaat tatcaagcgg ttaggcatta atagcgataa
      361 cttcgccagc ggtaagcaat gtttaacatt tgagcaactt gttcagggtg ggagctatac
      421 ctgggatatc ggggtttttc gtctcgattt cagtgtcccg caggcctggg tggaagaact
      481 ggaaagtggc tatgttccac cggaaaactg ggagcggggt attaatgcgt tttatacctc
      541 ttattatgtg agtcagtatt acagtgacta taaagcgtcg ggtaatagta agagtacata
      601 tgtacgtttt aacagcgggt taaatttact ggggtggcaa ctgcattctg atgccagttt
      661 cagtaaaaca aataacaatc caggggcgtg gaaaagcaat accctgtatc tggaacgtgg
      721 atttgcccaa cttctcggca cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt
      781 tgattctgtt cgcttcagag gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc
      841 gaaacaaaat tttacgccac gggtgcaggg gattgctcag agtaacgcgc tggtaactat
      901 tgaacagaat ggttttgtgg tttatcagaa agaggttcct cctggcccgt ttgcgattac
      961 agatttgcag ttggccggtg gtggagcaga tcttgatgtc agcgtgaaag aggcggacgg
     1021 ttcggtaacc acctatctgg tgccttatgc agccgtgcca aatatgctgc aacccggcgt
     1081 gtcgaaatat gattttgcgg cgggccgtag ccatattgaa ggggcgagca aacaaagtga
     1141 ttttgtccag gcgggttatc agtatggttt taataactta ttgacgctgt atggtggctc
     1201 gatggtcgcg aataattatt acgcgttcac tttggggact ggctggaata cacgcattgg
     1261 tgccatttcc gtcgatgcca cgaagtcgca tagtaaacaa gacaacggcg atgtgtttga
     1321 cgggcaaagt tatcaaattg cctacaacaa atttgtgagc caaacgtcga cgcgttttgg
     1381 tctggcggcc tgcgttattc gtcgcgtgat tatcggacat ttaatgatca cgtttgggct
     1441 aacaataaag ataattatcg ccgtgatgaa aacgatgtct atgacattgc cgattattac
     1501 cagaacgatt ttggccgtaa aaatagcttt tctgccaata tgagccagtc attgccagaa
     1561 ggctggggtt ctgtgtcatt aagtacgtta tggcgagatt actgggggcg tagcggcagt
     1621 agtaaggatt atcagttgag ttattccaac aacctgcgac ggataagcta taccctcgcg
     1681 gcaagccagg cttatgacga gaatcatcat gaagagaaac gttttaatat ttttatatcg
     1741 attccctttg attggggtga tgacgtttcg acgcctcgtc ggcaaatata tatgtctaac
     1801 tcaacgacgt ttgatgatca ggggtttgcc tcaaataata cgggattatc aggaacagta
     1861 gggagtcggg atcagttcaa ttatggtgtc aacctgagtc atcaacatca gggaaatgaa
     1921 acgacagctg gagcgaattt aacctggaac gcgccggttg cgacagtgaa tggcagttat
     1981 agtcagtcga gtacttatcg acaggctgga gccagtgttt cagggggcat tgtcgcctgg
     2041 tcgggtggcg ttaatctggc gaaccgtctt tccgaaacgt ttgctgtgat gaatgcgcca
     2101 ggaattaaag atgcttatgt caatgggcaa aaatatcgca caacaaaccg taatggagtg
     2161 gtggtatacg acggaatgac accttatcgg gaaaatcacc tgatgctgga tgtgtcgcaa
     2221 agcgatagcg aagcagaatt gcgcggtaac cggaaaattg ccgcctctta tcgcggcgcg
     2281 gttgtactgg ttaattttga taccgatcag cgcaagccct ggtttataaa agcgttaaga
     2341 gcggatgggc aaccattaac gtttggttat gaagtcaatg atatccatgg tcataatatt
     2401 ggcgttgtcg gccagggaag tcagttattt attcgcacca atgaagtacc gccatcggtt
     2461 aatgtagcaa ttgataagca acaaggactt tcatgcacaa tcaccttcgg taaagagatt
     2521 gatgaaagta ggaattatat ttgccagtaa tagtcaggtg gttatgaata ttcgtataat
     2581 gttatttata gtaatgatga tggttatgcc tgtgagttat gctgcatgtt atggcgagtt
     2641 gtcagcacag cataatttgg ttgttcaggg ggattttgcg ctaactcaaa atcaaacagc
     2701 aactcataca tatactttta atgatacttc ttgcgtcagt actaatacag tagcgcagat
     2761 agacccctcg gatattgtga ttggactcta taatgacacc atcaaactta agttgcattt
     2821 tgaatgggag aaaaaaggta gcatcacgct ttcaaataac caaactagtt ttacgagtgg
     2881 atataatgtc actgtgacat cggcagcgag cgatgccata gtgaatttgt ctggtggggg
     2941 aagtggatct ctcatgatta acagtgtcgc gacattaagc agtgcttcat catctactcg
     3001 tttggctgct tttttacaat ttgcaggttg ttttctaggc ggcacctcat gggatatctg
     3061 tgtgcaaaac tataaaaatg cattatcaaa aaatgcaggt gtctattcct ttaatctggc
     3121 attgt
//
LOCUS       DAZKFU020000371         3082 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZKFU020000371 DAZKFU020000000
VERSION     DAZKFU020000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.373"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            66..959
                     /locus_tag="ACHOQQ_RS23345"
                     /old_locus_tag="pgaptmp_004669"
     CDS             66..959
                     /locus_tag="ACHOQQ_RS23345"
                     /old_locus_tag="pgaptmp_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4472810.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLRKYPEISVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINIRLPTAGGLYHWEFEREGKALRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYITSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(990..1979)
                     /locus_tag="ACHOQQ_RS23350"
                     /old_locus_tag="pgaptmp_004670"
     CDS             complement(990..1979)
                     /locus_tag="ACHOQQ_RS23350"
                     /old_locus_tag="pgaptmp_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4472811.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGSIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2006..2857)
                     /locus_tag="ACHOQQ_RS23355"
                     /old_locus_tag="pgaptmp_004671"
     CDS             complement(2006..2857)
                     /locus_tag="ACHOQQ_RS23355"
                     /old_locus_tag="pgaptmp_004671"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4472812.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLHAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
BASE COUNT          743 a          755 c          798 g          786 t
ORIGIN      
        1 aatagcatct atgccccccc ttcatgagtg tctgtgaaga ggatgtttct acaaggaaat
       61 cgccaatgct caaagaaaac ttcaacgaac tgcaaatctt tcttgtggtg gcaagagagc
      121 gaagttttac caaagcagcg ggcaaactcg gcgtctctca gtctgcactt agccatgcaa
      181 tgaaggctct ggaggaaagg ctcaatatcc gcctcctgac acggactacc cgcagtgtgg
      241 ctcccacaga agcgggggag agaatcattg cctgccttga accgcgtatt gacgagcttg
      301 aacaggaact ggagtcactt attcaacaga acggcacgcc ctccgggaat attcgtttat
      361 ctgccgggga acatgccgcg cgaagtctgg tctggccgaa gctaaaacct tttctcagaa
      421 agtatcccga aatcagtgtc gaactggtgg tggataacgg ttttgtcgat attgttgaag
      481 gccgttttga tgccgggata cgactgggtg aaaatgttga taaagatatg gttgcggtca
      541 gaattggacc agatatgcgg atggctgtgg tgggggcacc ggcgtatttt gctgcaaacc
      601 ctgcgcctga aacgcctcac gagctacaaa accatcgctg catcaacata cgtttgccaa
      661 ctgccggtgg actttaccac tgggaattcg agcgagaagg gaaagcgctt cgcgttaagg
      721 ttgatgggca gctgacgtgt agtccattgc cagaaagaat tgatgccgcg ttatcagggt
      781 ttgggatcgc ttgtgtccct gaagacatgg ttcaggagta tataacgtca ggcaagctta
      841 ttcaggtgtt gcaggagtgg tgtccaactt ttcccggata ttatctctac taccccagcc
      901 gcaagcagca tccgcctgct tttgcgctgt tgatcgatgc gcttcgctac accgaataac
      961 gggtcctttg tccaggaccc gtgtgggtat tagcggccta cgcgtgcctg gtgttcaggc
     1021 gagtaacgtt caccgacgat tttaattgtt tccagcgcct cggttatctg ctgcgtatct
     1081 ttctgcgaaa gaacaatgtc agcagcggcc aggttttctt ccagccggtg cagtttagtg
     1141 gtgccaggga tgggaacaat ccacggcttt tgtgctaaca gccatgccag tgcgatttgt
     1201 gcagacgtta cccctttctc cgcagccagt tcacctaaca aggtgaccag tttttcatta
     1261 gcttcaatgg cttgtgctgc gaaacgcggt acggtgctgc ggtaatcatc tttgccgaaa
     1321 gtggttcccg gcttaatcga cccggtcagg aaacctttac ctagtgggct gaaggggaca
     1381 aaaccaatcc ccagttcctc cagcaacggc agaatttcct gttcaggctc acgccaccac
     1441 atggaatatt cgctttgcag ggcggtgaca ggttgtaccg catgcgcacg acgaatggtt
     1501 tgtgcgccag cctcggacag accgaaatgt ttgaccttac cttctgcgat gaggtctttc
     1561 accgttcctg caacatcttc aataggaaca tccggatcaa cacggtgttg gtacagcagg
     1621 tcaataacat ctgtcttcag acgacgtaat gatccttcaa cggcttcacg gatatgttcc
     1681 ggacggctgt ttaaaatctg ctgcttatta tcatcgccga aggtaaaacc gaatttagtg
     1741 gcgatcacca cgcggtcacg aaacggtttt aaggcttcgc cgaccacttc ttcattcaga
     1801 tacggaccat acacttcggc ggtatcgaag aaggtgacgc cacgttcaac cgcagcacga
     1861 ataagttcga tagcctgacg ggtatcggtc gccgggccgt agccgtggct taagcccatg
     1921 caaccgagcc caagggcgga aacttcgagt ccggatttac ccagataacg tttttgcatt
     1981 gaatgaatac ctcttttatc gcggcttaaa catcaagttt gcggccagtc agccattcca
     2041 ccattgcggg atcgcggtga gagaagaacg cgctggttgc ggtatccagg gcggtaattt
     2101 gcagcatatc ttcaggactg agttcaaaat cgagaatgtt gatgttctct tccatgcgtt
     2161 ctttgcgtac tgattttgca agcgaaacaa tgcctcgttg gtagatccag cgcagaacaa
     2221 cctggcctac gcttttgcca tacttctcgc caattgccgt gagaacagga tgctggaaca
     2281 gaccattttt cccttccgca aacggtgccc aggcttctgg ctgaatacca cggctttgca
     2341 tccacggaac ggcgtgtagc tgctgattga aagggttaac ttcaacctgg tttaccgctg
     2401 gaacgacgtt attaaaggcg ataaggtcag ccagtcggtc aggatggaag ttactgacgc
     2461 caatagcgcg aattttaccg gcctgttgca gctcctccat ggcacgccag gcaccgtgga
     2521 catcaccgta aggctgatga atcaaataca ggtcaacgta atcaagttgc agtcgattca
     2581 gagagcgttc gaactgggct ttggcccctt cgtaactggt atcctgcaac cagagtttcg
     2641 tggtgacaaa gagttcgtta cgggcgatac cgctttgttt cagtgcgttc ccgacctggg
     2701 tttcattttg ataggaggcg gcggtgtcaa tcagtcgata accggtgtta atagcgtcaa
     2761 tgaccgctct ttcgcattca gcggcatcgg tcatctgaaa tacaccaaag cccaacaggg
     2821 gcatttcaat accgttgttc agttttactt tttgcatggt gttatccttc cgttctcgtt
     2881 catgagaagc ataacgtaaa gggaaaagct cgattagacg gcaggatttg tcaggggtta
     2941 tgaacgaaat tcataaatct gtttgagtgt tgcgatgggt agtgcaagtt cgatatctcc
     3001 gcaatttaca gtccgatgaa ggaaaatgaa tatccataaa aaatatatcg gtttatcctg
     3061 gcgtatatac ctacttcgac gt
//
LOCUS       DAZKFU020000372         3077 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZKFU020000372 DAZKFU020000000
VERSION     DAZKFU020000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.374"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            115..591
                     /gene="agaB"
                     /locus_tag="ACHOQQ_RS23360"
                     /old_locus_tag="pgaptmp_004672"
     CDS             115..591
                     /gene="agaB"
                     /locus_tag="ACHOQQ_RS23360"
                     /old_locus_tag="pgaptmp_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGU4472813.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            630..1433
                     /gene="agaC"
                     /locus_tag="ACHOQQ_RS23365"
                     /old_locus_tag="pgaptmp_004673"
     CDS             630..1433
                     /gene="agaC"
                     /locus_tag="ACHOQQ_RS23365"
                     /old_locus_tag="pgaptmp_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGU4472814.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1423..2214
                     /gene="agaD"
                     /locus_tag="ACHOQQ_RS23370"
                     /old_locus_tag="pgaptmp_004674"
     CDS             1423..2214
                     /gene="agaD"
                     /locus_tag="ACHOQQ_RS23370"
                     /old_locus_tag="pgaptmp_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGU4472815.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2215..2970
                     /locus_tag="ACHOQQ_RS23375"
                     /old_locus_tag="pgaptmp_004675"
     CDS             2215..2970
                     /locus_tag="ACHOQQ_RS23375"
                     /old_locus_tag="pgaptmp_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HGU4472816.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQHVDVSQLTVVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTSIPASFLWLHSNFICLINT"
BASE COUNT          784 a          711 c          751 g          831 t
ORIGIN      
        1 gtgccttaat ggaaaaggag atgactaaac cttaataaat acatcagtac aatatcgcaa
       61 caataatata tttaaaaaca ttatattatt caactttatg gtgaggatta cacaatgagc
      121 agtccaaata ttctcttaac ccgtattgat aaccgtctgg ttcacggtca ggttggcgtg
      181 acctggacat ccaccatcgg tgcaaatctg ctggtagtcg tggatgatgt tgtcgctaac
      241 gatgatattc aacagaaatt aatgggtatt accgcagaaa cctacggctt tggcattcgt
      301 ttctttacta tcgaaaaaac cattaacgtc atcggcaaag ctgcaccgca tcagaagatc
      361 ttcctgattt gccgtacgcc acaaacggta cgtaaattgg tagaaggtgg cattgacctg
      421 aaagatgtca acgtcggcaa tatgcatttc tcggaaggga aaaagcaaat cagcagtaaa
      481 gtttatgtcg atgaccagga tctcacggac ttacgtttta ttaaacaacg tggcgtgaat
      541 gttttcattc aggacgtccc tggcgatcaa aaagaacaaa tccctgacta aatctaaaat
      601 cgccttaata ttggtttgag gtaataaaaa tgcatgaaat aaccctactt cagggattat
      661 ccctggcggc gttagttttc gttctgggga ttgatttttg gctggaagcc ttatttttat
      721 tccgcccgat aatcgtttgt accctaactg gcgctattct cggtgatatt cagactggct
      781 taattaccgg tggtctgaca gagttggctt tcgccggatt aacccctgca ggtggtgttc
      841 agccgcccaa ccctattatg gcgggtctga tgaccaccgt cattgcatgg tctacgggcg
      901 ttgatgccaa aacggcaatt ggtcttggcc taccgtttag tttgttaatg cagtacgtca
      961 ttctgttctt ctattctgct ttctcattat ttatgaccaa agccgataaa tgcgcgaaag
     1021 aagcggatac ggcagcgttt tcccgactta actggacaac gatgctcatc gtcgcttcag
     1081 cgtatgcggt gattgctttc ctctgtactt acctggcaca aggggcgatg caggcgctgg
     1141 tgaaagcgat gcccgcctgg ctgacccacg gctttgaagt ggctggcggt attctgcctg
     1201 ccgttggttt tggcttgctg ctgcgcgtga tgttcaaagc acaatatatc ccttacctga
     1261 tcgccggctt cctgtttgtt tgctacatcc aggtcagcaa cctgttgccg gttgccgtac
     1321 tgggcgcagg ctttgcggtg tatgagtttt tcaatgcgaa atcccggcag caagcgcaac
     1381 cgcagcccgt tgccagtaaa aatgaagaag aggactacag caatgggatc tgaaatcagt
     1441 aaaaaagata tcacccgtct gggctttcgt tcgtcgctgc tgcaagcgag ctttaactac
     1501 gaaaggatgc aggcgggcgg ttttacctgg gcgatgttgc cgatcctgaa aaagatttat
     1561 aaggatgaca aaccgggcct aagcgcagcg atgaaagata acctcgaatt tattaatacc
     1621 cacccgaatc tggtcggatt cctgatgggg ttattaattt cgatggaaga aaaaggagaa
     1681 aaccgcgaca ccattaaagg cctcaaagtg gcactgtttg gcccaatcgc cgggattggc
     1741 gatgcgattt tctggtttac tttgttgccg attatggcgg gaatttgctc atcatttgcc
     1801 agccagggaa acctgctggg gccgattcta tttttcgccg tttacctgct tatctttttc
     1861 ctgcgcgtcg gctggaccca cgtcggttat tcagtcggcg tgaaggcgat cgataaagtg
     1921 cgagagaact cgcagatgat tgctcgctcg gcaaccatcc tcgggatcac ggtaatcggc
     1981 gggctgatcg cttcgtatgt gcatattaac gtggtgacat cgtttgccat cgacagtacc
     2041 cacagcgtcg cgctgcaaca ggatttcttc gataaagtct tcccgaacat tttaccgatg
     2101 gcctacaccc tgctgatgta ttacttcctg cgggtgaaaa aagcgcatcc ggtgctgtta
     2161 atcggcgtga cttttgtgct ctctattgtt tgttccgcat tcggcatttt gtaaatggaa
     2221 cgaggcactg cgtctggcgg tgcctcttta cttaaggaat ttcatcctgt gcaaaccctt
     2281 cagcaagttg aaaactatac ggcgttaagt gaacgtgcca gcgaatattt attggccgtg
     2341 atccgtagca aaccggatgc cgtgatttgc ctggcgaccg gagccacgcc attactgacg
     2401 tatcattatc tggtagaaaa aatccaccag cagcacgttg atgtcagcca gctcaccgtc
     2461 gtcaaactcg atgaatgggt ggatctgcca ttaacgatgc ctggtacctg cgaaactttc
     2521 ctgcaacagc atatcgtgca gccgctgggg ctacgtgaag accagctcat cagctttcgc
     2581 tccgaagaga taaatgagac agagtgcgaa cgggtaacga acctgattgc gcgcaaaggc
     2641 ggtctggatt tatgcgttct cggattgggg aaaaacggtc atcttgggct gaacgaaccg
     2701 ggggaaagcc tacaaccggc ctgccatatc agtcaacttg atgccagaac acagcaacat
     2761 gagatgttaa aaaccgcggg tcgccccgtg actcgtggga tcaccttagg cctgaaggat
     2821 attctcaatg cccgcgaggt tttgttactg gtgactggcg aaggaaagca ggatgcgaca
     2881 gaacgttttc tcacggctaa agtctctacc tctatcccgg cttcgttttt gtggctgcac
     2941 agtaacttta tttgtttaat taatacctaa ccagatgaaa aatttgctca aaatgtttaa
     3001 attaacttat tcaacagtca cgcattatat taaataacat ttgactgggt tgaacataac
     3061 gccaatagca aaaggag
//
LOCUS       DAZKFU020000373         3055 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZKFU020000373 DAZKFU020000000
VERSION     DAZKFU020000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.375"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..646)
                     /locus_tag="ACHOQQ_RS23380"
                     /old_locus_tag="pgaptmp_004676"
     CDS             complement(<1..646)
                     /locus_tag="ACHOQQ_RS23380"
                     /old_locus_tag="pgaptmp_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HGU4472817.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKRFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIH"
     gene            839..1342
                     /locus_tag="ACHOQQ_RS23385"
                     /old_locus_tag="pgaptmp_004677"
     CDS             839..1342
                     /locus_tag="ACHOQQ_RS23385"
                     /old_locus_tag="pgaptmp_004677"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4472818.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPVIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1388..1804)
                     /gene="rraB"
                     /locus_tag="ACHOQQ_RS23390"
                     /old_locus_tag="pgaptmp_004678"
     CDS             complement(1388..1804)
                     /gene="rraB"
                     /locus_tag="ACHOQQ_RS23390"
                     /old_locus_tag="pgaptmp_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGU4472819.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1966..2970
                     /gene="argF"
                     /locus_tag="ACHOQQ_RS23395"
                     /old_locus_tag="pgaptmp_004679"
     CDS             1966..2970
                     /gene="argF"
                     /locus_tag="ACHOQQ_RS23395"
                     /old_locus_tag="pgaptmp_004679"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGU4472820.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSN
                     "
BASE COUNT          889 a          706 c          682 g          778 t
ORIGIN      
        1 gatgtatacc gaaactggta ttgctccaga gaaaactcat taccaggcta tataaagtac
       61 cattaaaaat accaatggaa acaataccaa ctattgccat cagaaccaca gatataataa
      121 cactacttga actattggca tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc
      181 caatggccat taaaatcggc aagaaaaagg ttacccacca gaaccttttc atagagcaat
      241 taaaactaaa tcttacgccg ttgagactgg tcatcaaggc ctgataacgc agtccttttg
      301 ctgccataaa tataagcaaa accaacagtg acaaagtcaa aacacagcca acaagcggca
      361 tatctgctga cactgtcatt aagattgcga aatagaaaaa aacaaaaaca agacaactaa
      421 aaaaaacatt cccaccagta attccataag aaaatcgttg tccgttaact tccatattag
      481 catagagata acgcttacat ttcattaatg cccatggtaa atagatacct agcgttataa
      541 tcgttaacaa cacattcacc acacaaataa gaaaatatct ctcacctgta ccatgaaaaa
      601 caaacgaatg ggacggaaca tccatttcat taataacttg agccataaaa attccctgaa
      661 ttaaaaaatc cattgatata atttatagat acagacagta aaatgttctg ccacgttaca
      721 atactcttat cagtaatagg ctggcaaaaa ctttattgct ttataagatg cttgcgcacg
      781 taagaagaga gtggtattaa acaaatgtat aattacttta tttttattgg gttaaactat
      841 gaataacatt gcgccgcaat cacctgtaat gcgtcgcctg acgctgcagg ataatcctgt
      901 tattgcccgc gtcattcgtc aggtatccgc cgaatacggt cttaccgctg ataaaggcta
      961 caccgtcgcc gatccgaatc ttgacgaact gtatcaagtg tatagtcagc cgggtcatgc
     1021 atattgggtg gttgaatacg agggtgaagt ggtcggcggc ggtgggattg cgccattagc
     1081 aggtagtgaa tcggacattt gcgaactgca aaagatgtat tttcttccgg ctattcgcgg
     1141 taaagggctg gcgaaaaaac tggccttaat ggcgatggag caggcacgag aaatgggctt
     1201 caaacgctgc tatctggaaa cgaccgcttt tttaaaggaa gccattgcgc tttatgagca
     1261 tttgggcttt gagcatatcg actatgcgct tggctgcacg ggccatgtcg attgtgaagt
     1321 gcggatgcta cgcgaactct aaaaaaatgc ccgctagtgt gtgctatcgg gcattttaat
     1381 caattaatta gtggcgaacc ccgtcatcgt cttcatcgac aaaatcttca tcgtcgccat
     1441 cttcgccgtt aggatcttca aagtaagtgc cccaaccgtc gtactcaacg tcaaatttct
     1501 ccgccagcgt catcagttgt tcaacctggg catcgatcag atcggcattc aacgcgcact
     1561 cgctgaggat gtcgcagcaa atcacgatat caccatcttc aacttccagc tcttctggat
     1621 cggtcacttc gtaaccgagt ttaaacgctt caactgctgc tttttccagg gtttccagat
     1681 cgtctgcgga aagatggtgt tcgatggtgt acagcgcgtc cggatcgctg ccatcttcca
     1741 gtaattcttc aataatcaaa cgtgtttctt cacgctgttc ttccagttgt tccgggtttg
     1801 ccatggctca ttcctcttaa tgtcctgccg atactcatat tgtcacacac cactggcatt
     1861 gcctccacct ttgtgacaaa gatttatgct ttagacttgc aaatgaataa tcatccatat
     1921 aaattgaatt ttaattcatt gaggcgttag ccacaggagg gatctatgtc cgggttttat
     1981 cataagcatt tcctgaaatt actcgatttc acgccagctg aactcaacag cctgctgcag
     2041 ttagccgcga agctgaaagc cgataagaaa agcggcaaag aagaagccaa actcactggt
     2101 aaaaacatcg cgctcatctt cgaaaaagac tcgacccgta cccgatgctc tttcgaagtt
     2161 gccgcatatg accagggtgc tcgcgttact tatctcggcc caagcggcag tcagattggt
     2221 cataaagagt cgattaaaga cactgcccgc gtgctgggtc gcatgtatga cggtattcag
     2281 tatcgcggct atggtcagga gattgtcgaa acactggcgg aatacgctgg cgtgccggta
     2341 tggaacggcc tgaccaatga gtttcacccc acgcagttgc tggcggatct cctcaccatg
     2401 caggagcatt tacccggtaa agcattcaac gaaatgacgc tggtctatgc aggtgacgca
     2461 cgtaacaaca tgggcaattc gatgctcgaa gctgcggcgc ttaccggtct ggatttgcgt
     2521 ttggtcgcgc cacaggcatg ctggccggaa gctgcgctgg ttacggaatg ccgcgctctg
     2581 gcacagcaaa atggcgggaa tattacgctg acggaagatg tagctaaagg cgttgaaggt
     2641 gctgacttta tctataccga tgtatgggtg tcgatgggtg aagcaaaaga gaaatgggca
     2701 gaacggattg cattactgcg tgattatcag gtgaacagca agatgatgca gttgaccggt
     2761 aacccggagg tcaaattcct ccactgcctg cccgcgtttc atgacgacca gacgacgctt
     2821 ggtaagaaaa tggcggaaga gtttggccta catggcggaa tggaagtgac tgatgaggtc
     2881 ttcgaatctg ccgccagcat tgtttttgat caggcggaaa accgtatgca taccatcaaa
     2941 gcagtgatgg tcgcgacgct cagtaactaa ttgatatcgc tataaagatg ccgggaaata
     3001 caaaactatc ccggcatcac attattttat gcagtgatag gtctattctt ccacc
//
LOCUS       DAZKFU020000374         3054 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZKFU020000374 DAZKFU020000000
VERSION     DAZKFU020000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.376"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..921)
                     /gene="rfaC"
                     /locus_tag="ACHOQQ_RS23400"
                     /old_locus_tag="pgaptmp_004680"
     CDS             complement(<1..921)
                     /gene="rfaC"
                     /locus_tag="ACHOQQ_RS23400"
                     /old_locus_tag="pgaptmp_004680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGU4472821.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADL"
     gene            complement(925..1971)
                     /gene="rfaF"
                     /locus_tag="ACHOQQ_RS23405"
                     /old_locus_tag="pgaptmp_004681"
     CDS             complement(925..1971)
                     /gene="rfaF"
                     /locus_tag="ACHOQQ_RS23405"
                     /old_locus_tag="pgaptmp_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGU4472822.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(1981..2913)
                     /gene="rfaD"
                     /locus_tag="ACHOQQ_RS23410"
                     /old_locus_tag="pgaptmp_004682"
     CDS             complement(1981..2913)
                     /gene="rfaD"
                     /locus_tag="ACHOQQ_RS23410"
                     /old_locus_tag="pgaptmp_004682"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGU4472823.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
BASE COUNT          727 a          835 c          744 g          748 t
ORIGIN      
        1 caaatctgca agagacatac ttgtagaacg acactctact tgattcttcc catatccgcc
       61 aattaatccc ggatcggttg gtccataaac cgtgatattg ggtctatcca gtgccgccgt
      121 taaatggctt aaccccgtat ccaccgacac tacaaattta gccccggcca gcacgcgggc
      181 aacgccttcc agactcatct tcggcaatac ttcaacataa gcaaatcctt ccgccagtcg
      241 tttcgcccgt tcttcctcat gcggcgcgcc ccacggaagt ttaatccgta ttcctgaatc
      301 agccagtaaa ccaatcaatt ctcgccagtg ttcttccggc cagtgtttat catcacgggt
      361 cgtcgcatga agaaatacgg catattcgcc agcatctgta ggcaggttcg tcagaaaatg
      421 ctgtgcgata gcataatcgc cctgggtttg cggtttgcta tagcccaaac ttttggcaaa
      481 tagttcgcgg gtgcgttcta cggcgtgctg ctgttttgca atatgatgct tacgattgta
      541 aaacaggctg gctaaaggtt cgcgagcggt ttgccagtcc atgccatgct ttacgccatg
      601 cgccagacgc gtcaccagcg ccgcgctttt taccagcccc tgagcgtcga taactgcgtc
      661 atagttctct gcttgtagcg cttcacgaaa cgctttgcgt tccgctttta tgggggccga
      721 gaaccaggct ttacgccagc gacgtattgc cacaggaata actcgctcaa cggcagcgtg
      781 ccaggaagga atctgtgcga acccttcttc caccacccag tcaaacttaa tccctgggat
      841 tgcctgctgg gcatcagtga gtgcgggcaa cgtatggaga acatcgccca tcgacgatgt
      901 tttaacgatc aaaacccgca tccgtcaggc ttcctcttgt aacaatagcg cgttgagttc
      961 ttccagtacg cgctggggag taatgtcgat taagctctgg tgataaccct ccgcagcgtc
     1021 acctttacgc actttgtgat agccggtaat caaacggatc acgcgcgctt tatgggatag
     1081 cggcggtgtg aagtccgggc tactcggacc atacagggca accagcggac gattgagcgc
     1141 cgccgcaaca tgcatcaggc cagaatcgtt agtgacaatg gctttacagg ctgcaatcag
     1201 gataaccgct tgatcaagct gtgtttcccc cgccaggttc cgacaccatg cctgctgctc
     1261 ggtattcaaa gcggcaagaa tctcattgcc cgcttcatga tctttcgccg agccaaacag
     1321 aaccacctga taaccttcat caatcagctg ctttgccagc tccgcatagt ggtagtgtgg
     1381 ccagcgtttt gccggaccaa actccgcacc cgggcaaaaa ccaatcatcg gacgttctga
     1441 tgaaagcgaa aattgattac aggtatatga tttttcacct tcgctcacct gcaactgcgg
     1501 ccataacaat ggctgcggca gatcttgtgc tgtgcgcata atgcctttgt cataggccag
     1561 cgctatatag cgttccacca ttagcggcca ggcttcttta tcgagcacgc gtacatcgtt
     1621 gagtaaaccg tagcgcatct cgccgcgcca gccggtgcga tgaggaatac ccgcgaagaa
     1681 aggcactaat gcagatttga aggagttggg taagacgtag gcgcggtcgt agcgcttttc
     1741 acgcagacta tgacccagtt tgcggcgttc gccgatttcc agcgctccgt gaccgagagg
     1801 cataggaata gcttcgttaa cttccggcat ccgcgataat aatggacggc accatgccgg
     1861 tgccatcaca tcgattatcg cctggggata gcgcgcctgg agcgtgcgat agagactttg
     1921 cgacatcatc atgtcgccaa cccaagacgg gccgatcacc agtattttca tgcagagctc
     1981 ttatgcgtcg cgattcagcc aggccatgta ttccgttaca ccttcagcaa cggttttgaa
     2041 cggtttgtcg taacccgccg cgcgcagatt tgtcagatct gcctgagtga acgcctgata
     2101 gcggcctttc agtttatccg ggaacggaat gtattcgatc tggcctttct tgtgataagc
     2161 cagcgtagca tcagctacag cctggaagga ttccgcacga ccagtaccga gattgaagat
     2221 gccggaaacg ccattttcca ggaaccacag attcacatca gccacgtcgc ccacatagac
     2281 gaagtcgcgt ttgaagttct cgctaccttc aaacagcttc ggtgattcac cgttgttaag
     2341 ctgggtgttg agatggaaag cgacgctcgc catgctgcct ttatggcctt cacgcggtcc
     2401 ataaacgttg aaataacgga agccaacaat ctgcgagttc gcttcaggca ggatttgacg
     2461 aacatattca tcaaacagga attttgagta accgtagacg ttcaacggtt tttcgtactc
     2521 gcgggattca ataaagtcgg aggtgcgtcc gccgtaggtg gccgcggaag atgcatacag
     2581 gaacgggatt tcgcgctcca gacagtagtg cagcagctct ttggagtatt gatagttgtt
     2641 atccatcata tacttgccat cccactcggt ggtggaagag catgcgcctt cgtggaaaat
     2701 cgcttcgaca tcgccgaact cttcgccagc cataatctgg atcaggaagt cttccttatc
     2761 catatagtcc gcgatatcca gatccaccag gttcacaaac ttggtgccgt ctttcaggtt
     2821 gtccaccacc agaatatcgg tgatgccttt atcattcagg gccttaacga tgttgctgcc
     2881 gataaagccc gcgccgccgg taacgatgat cataactgta accttcgaat tatggagtca
     2941 gagacaatct cagacatgaa tgtctgtaat cataccatca catccgaatc ccttcagccc
     3001 tttacataga gatgcaagga tgctaattgc aacaaactag tttgggtttt agct
//
LOCUS       DAZKFU020000375         3035 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZKFU020000375 DAZKFU020000000
VERSION     DAZKFU020000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.377"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..726)
                     /locus_tag="ACHOQQ_RS23415"
                     /old_locus_tag="pgaptmp_004683"
     CDS             complement(<1..726)
                     /locus_tag="ACHOQQ_RS23415"
                     /old_locus_tag="pgaptmp_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4472824.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISA"
     gene            complement(794..2275)
                     /locus_tag="ACHOQQ_RS23420"
                     /old_locus_tag="pgaptmp_004684"
     CDS             complement(794..2275)
                     /locus_tag="ACHOQQ_RS23420"
                     /old_locus_tag="pgaptmp_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HGU4472825.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGRWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPLAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVQSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESGIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFF
                     QARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            complement(2268..>3035)
                     /locus_tag="ACHOQQ_RS23425"
                     /old_locus_tag="pgaptmp_004685"
     CDS             complement(2268..>3035)
                     /locus_tag="ACHOQQ_RS23425"
                     /old_locus_tag="pgaptmp_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4472826.1"
                     /translation="RLWLTQSFRFGPAVARMANLLLQREGETREVKGCGGDDEVLLKC
                     HACEHLQGHYTVLSRTVAGVIGTALMAAMKGQKVYWVGGIEGYRTGELEDLYWFQVDM
                     PERMHSDRLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLELLRQYTVTN
                     EQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLYVAVTRALRT
                     LVLNDLMHQLAEEQQKTEGLRGTGYDTSGRVQENGHA"
BASE COUNT          715 a          969 c          612 g          739 t
ORIGIN      
        1 ggcagagatc ggctcactga aacgacagac gtcaaaaaga tagtcatcat cacctgcccc
       61 gcactgacgg gcccgctcca gcagtttttc catcgatacc ttcaggggag ccaccagagg
      121 aacgccccat tccctgtgag ttttacttcc ctccagctta agatcaatta tgctttcatc
      181 cggacagaca tccctcacac ggatatgctt cagttgatta aagcgcattc cggtataacg
      241 cagaacatcc agaactgtca gccagtacca ggcagggtat atagcattgc gtcgacgact
      301 attcaccatg ttctgctctt ccgcttctgc aaacctctgc acaaccaggt aaaccctgag
      361 catctgattg tcactcagtg tctttttctt tttttcagta ctcgcaactg atgcttccac
      421 caacgggttt tctgtctgag gcagcagttt catcttgatc ccgaatccca taattgcccg
      481 caagtgggcc accttgttat tccaggtcac ctccctgcgc ttctgctctt tcaggacata
      541 acgccggtat cgctgaacat cacggacagt cacctccccc ggcaaaatat caccgataaa
      601 ttccctgaat accctgacaa ctttgcagta gctccactct gtatccggcc gaagattctt
      661 tgaaaaaaag tactcttcca gcagttgttc ccaggtgatt tttgtgacgg aatcgtcatt
      721 ctgcattata tattcccctt gtttactgtg ctcccgcact ctccggtcaa attctgcaca
      781 tcatcgcctt tactcacccg aaaacaataa gcgggctgtc tccgggaacc gtaatattcc
      841 cgtacagaaa cctgccctta accagatatc ccgaaaccat tttgtaacgt ccggaatgtt
      901 ctgcgtcact ataaagacgg gcctgaaaaa aacgctctcc tgaacgtacc ttatggatac
      961 ctgcccgttc aaagagcgac tgaacataac gacgatcctg cccggaacca ctctccttca
     1021 gaaaacgata aaaaatcccc ggggcacaca gaaaaacata tccggaaaca acatgaacat
     1081 ttgaatcaac gctgttaacc gaaagtgaac cctccctcag cccctctttt aaccatctga
     1141 taaattcact ctctaccctg ctatcctctg tcagggaaaa ctctgccgtt ttttccactg
     1201 tttccgggat acggtttccc tgattttctg ttacacatcc gctctcttcc ggtaactcaa
     1261 taccgctttc tttatccggc ggcacgcctg acgctatctg ccctccctca tccgacaaat
     1321 cagcagcaga aaacagcgta aggagcattc cggtatcagc atcagtttcg ttcccctcat
     1381 ccacctcctg ttccgctgtc cggccctcat tttcggcatc ctccactggc gactgtacaa
     1441 ccggcaacgg ctgaatgacg ctttctgctg aaattgctgc tgactgcagc agtaccggaa
     1501 caacctcagc caccggaact tcggccacat ctcctgcagc gaggggactc acgacttcct
     1561 ccatagcttc ctgtaatact gtacgtatca gtgccatttc aggatgctga tgccagactg
     1621 ctccggcaag attctgtaat gcccccggga cagtggcaag ccaggctccc gcctctccgg
     1681 gcagcagttg tcccgccatc agggctgcca gttgccgtcc ctccgttccc tggcagggca
     1741 tcttcctgaa ccggaaccgg tatacgccct caggtacaga tatccccggc tgccagcatc
     1801 tgccgtcggc ctgttcgcct tccagttccg ccagcaacgg caggtgataa aacagagccg
     1861 accagaacac caccatgttc cacacactcc cctgcgcggc ctgctcttct gctgtcgctc
     1921 ccggtggaaa cattctgcct ctggccagcc ggacagcata cgtcgtgaac tgcagggtca
     1981 ggtcaccaaa accaccggtg taaccccagc gaccttctgc tgtggccggc agattctgac
     2041 tgacagacaa caactgatgc acaggatcaa gacaaaggcg ctgatacata tccaccggca
     2101 tggaactgtt ttcccataac tgtttcagac accttttcct gaacggtgtg gataccagta
     2161 catcagccgg caatggtgta aaatacccct ccggcaccgt ttccccggga atatccgtcc
     2221 ggacatcaga acattctgtc ttcacaggaa ataacctttt caggcgttca agcatgccca
     2281 ttctcctgaa cccgacctga tgtgtcgtac ccggtccccc gcagaccttc agttttctgc
     2341 tgttcttctg ccagttggtg catcaggtca ttcagtacca gcgtcctgag ggcccgggtg
     2401 acagccacat ataacagaca ggtttcatca tgacgctcgg cctccgtcat cagcggatcc
     2461 gtgatatcct ggaaatcctc atccagcatt acaaccggcc actccagccc cttgctccgg
     2521 tgtgccgttg acaccgtgat atctgcatcc tgctcattcg ttaccgtata ctgccggaga
     2581 agttccagtt ttttcggtaa cggaaaacac aactccagaa gccgaaggga ctgattcatc
     2641 tccacatcct tcgtggattt tgcaatatat ttatattctt caaaattcct gtagtcccgg
     2701 cgcagcctgt ctgaatgcat ccgttccggc atatcaacct ggaaccagta gagatcctca
     2761 agttccccgg tcctgtaacc ttcgattccc cccacccagt aaaccttttg ccctttcatt
     2821 gccgccatca gcgcagtacc aatcacaccc gcgacagtac gactgagtac cgtgtaatgc
     2881 ccctgcagat gttcacacgc atggcatttc agcaatacct catcatcacc accacaacct
     2941 ttcacctctc gtgtttcacc ttcacgctgc agaagcagat ttgccatacg agccaccgcc
     3001 ggaccaaaac ggaaactctg tgtcagccac aaccg
//
LOCUS       DAZKFU020000376         3001 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZKFU020000376 DAZKFU020000000
VERSION     DAZKFU020000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.378"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2151)
                     /locus_tag="ACHOQQ_RS23430"
                     /old_locus_tag="pgaptmp_004686"
     CDS             complement(<1..2151)
                     /locus_tag="ACHOQQ_RS23430"
                     /old_locus_tag="pgaptmp_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4472827.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSAKAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTDCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSTGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVTALTDNTQGA"
     gene            complement(2216..2815)
                     /locus_tag="ACHOQQ_RS23435"
                     /old_locus_tag="pgaptmp_004687"
     CDS             complement(2216..2815)
                     /locus_tag="ACHOQQ_RS23435"
                     /old_locus_tag="pgaptmp_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4472828.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDILGLVTSFSYAGDKNRQLSRYSDTRWHEDSVRNRWFSVMVG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
BASE COUNT          546 a          869 c          795 g          791 t
ORIGIN      
        1 tgccccctgc gtattgtctg tcagcgcggt tacccgacct tttgaagcca gattaactga
       61 tgatactgct gttccacctg acggtaacgc cccgatctct gatgccgttg gcttatttct
      121 ggagttatag tcccttcgcc agccaggtga ataatctgtt ccgtgattaa tataggtaaa
      181 ctgggcgtta gttgttccac caccagtgga ggtggtcggt gtggtaatgc ggatcgtcat
      241 cgctgacttt atccccatta cttcaatgac agctccggcg agatgaatat taccgcagtc
      301 agtatcagta atgattttat tattgccata agaccaggaa cccttgcaca tccagtaggg
      361 atggttaaat gctccctgag aatccagcca ctcgataaac tgtgcagtcg tccagtttcc
      421 tgttgttgtg cttactgacc caccgaaggc acggcaggca ccaatatttt tcgtaaaggt
      481 gtctttgcca gggatatccg caccgttctg atctttctgc agacgtttct cagcattgtc
      541 attggctgct tttactgcct ttggcgttgc cgccagcgtt tcagacgtgc tgttggtcgc
      601 gctgcttagc tggattatcc ctttctgtgc tgtcgttgca tcctgtgcgg tgtatttccc
      661 gttagccagg tcatacgcgg ccttaacggc ttttggtgtt gccgccagtg actcggaagt
      721 gctgttagtc gcactactga gctgtactat cccctttttc gtcgtgctcg catcctcaag
      781 cgccacggcg gatgcaatat cctctgcccg ttttgccgct gtctcagcgc gcgttgctgc
      841 agattccgcc gtacttttgc tctgagctgc tgccttcgca ctaccagcag cctctgtcgc
      901 cttcgtggat gccgtcgtgg cgctgccctt cgctgctgac gcctgtctgg tcgcctcatc
      961 ttttgaagca gacgccgatg atgccgatga cgccgccgaa ctggcggacg atgccgctgc
     1021 cgtttttgag gattctgcac gggtttccga cgctttcgcg ttcgtttcgg atgtcttcgc
     1081 tgcggaagct gacctcgctg ctgccgtggc ctgctcagtg gcttcgccag ccttcgttgt
     1141 ggctgttgaa gcggatgatg cggcgctttc tgccgatttt ccggcggcgg tggcactggc
     1201 tgaggcctgc ccggcacttg ttgacgcggc actggcagac gacgcagccg ctgtttttga
     1261 gcctgccgca gctgaggcgc tctgtcccgc tgccgtctca gaagacctgg cgttcgtctc
     1321 ggacgttttt gccgccttcg cggaatttcc tgccgccgtt gccgaggaag ctgcgctact
     1381 ggcgctcaag gatgcgttcg tttctgatga tttcgctgcc tcttttgagg ccgccgcatc
     1441 ccgggctgag gtggcagctt ctgacgcttt cgtggtcgcg gtggatgcag aagtggcggc
     1501 tgattgttct gacgctgcgg cattcgtttc tgacgttttc gccgcaccgg cactggtagc
     1561 tgccacgctt tttgaggact ctgcagcggc agcacttttt gaggcttcag tggcctttgt
     1621 tgatgccgtt cctgcgctgg aagacgctga ctgagccgac gaagcggcct gtccggctga
     1681 cgtgctggct gcacgtgctg aatctgcagc atcagtcgca tgggttgccg cctcacgggc
     1741 agatgtgctg gcatcactgg ctgacttctt cgcggctgcc gtgttctgtg ccaccacgga
     1801 cgcgttacgc gccacctctt ccaccatcag ttcaaaacgg cgcagtgcct caggacgggc
     1861 atcatcctcc gtcatggcac cgagaaaatc attcagcgta ccgggtcggg aatcttcata
     1921 cacggtgatg gtcccggcat gtgacggcgg gaatccttcc accaacagaa taacgctgta
     1981 ctgaccgtac tcaacgtcca tgctgtaacg cccggcttca tccggatttt ctgaggccag
     2041 cgtgttcacc accaccgtgg tgctgttacg ttttgctttc agctggattg tgcagttctg
     2101 taccggtttt cctgtgccgt ctttcagtac acctgaaatc tttactgcca tattcacccc
     2161 acaaaaaagc ccgcctgaac cggcgggctg tcataacact gtgttacctg gctaatcaga
     2221 acttataacc gacacccatg atgaaaccgt cagtgcgcca gtcgccactg ccggagcctt
     2281 cataagcaat atcaatggcc acggattcgg tcgggttaaa ctgcacgcca gctccccacg
     2341 ccagagacgt gttgctgtgg cgaccgtcat cacttccggt cagcacgtcg tgcgttttcc
     2401 ccttgttgtc agttacgcgg agataatccc cggagaaagt cgacacacgg ctgtaagcca
     2461 cacccgccat cgcatacgcg ctgaaccatt cattcacgcg cacagacggc cccaccatca
     2521 cgctgaacca gcggttacga acggaatctt catgccagcg ggtatcgctg taacggctca
     2581 gctggcgatt cttgtctcct gcatagctga atgacgtcac cagccccagt atgtccgtaa
     2641 actcataacg gtatttcacg ttaatcccgt tcagatcatc gctgccggga acgttcgtcg
     2701 aggcatgaag ataccccgcg ctcagcgtgg actgatgttc agacgcccat gcaggcgcac
     2761 cggatacggc cagacaaatg gctgcggaca aaatggcggc ataaagttta cgcataatta
     2821 cctctcgctt ttctgcaata aaaaaggcgt catttctgac gcccgttatg ggttataaaa
     2881 ttcagttgat actgatacct gctgtggatt tcttcatcac cacaaccagc agatcgctga
     2941 tactggttgt tggtgtccag ttattcgctc ctgatgaaga tacggtgaat gtcagtgtca
     3001 g
//
LOCUS       DAZKFU020000377         2991 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZKFU020000377 DAZKFU020000000
VERSION     DAZKFU020000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.379"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2991
                     /locus_tag="ACHOQQ_RS23440"
                     /old_locus_tag="pgaptmp_004688"
     CDS             <1..>2991
                     /locus_tag="ACHOQQ_RS23440"
                     /old_locus_tag="pgaptmp_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HGU4472829.1"
                     /translation="ELIQDLQNQGYEFISVKSQSAMLANVREQLQSLNGVVFNDSEWR
                     RFTEQYLDNPGDGILDKTRKIHIDYICDFIFDNGRLENIYLIDKKNLMRNKVQIIRQF
                     EQAGSHSNRYDVTILVNGLPLVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYL
                     QLFVISNGTDTRYFANTTKRDKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNV
                     LVNYSVFDSSQTLLVMRPYQIAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKT
                     LTSFKAARLATELDFIDKVFFVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLD
                     KDDNKIIVTTIQKLNNLMKAESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFRRYY
                     QFGFTGTPIFPENASGSETTASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKS
                     LETETDEKKLSAAENQQAFLHPMRIQEITQYILNNFRQKTHRTFPGSRGFNAMLAVSS
                     VDAAKVYYATFKRLQEEAANKSANYKPLRIATIFSFAANEEQNAIGEISDETFDTGAM
                     DSSAKEFLDAAIREYNDHFQMNFSTDSKDFQNYYRDLAQRVKNQDVDLLIVVGMFLTG
                     FDAPTLNTLFVDKNLRFHGLMQAFSRTNRIYDATKTFGNIVTFRNLEQATIDAITLFG
                     DKNTRNVVLEKSYAEYMEGFTDAATGEAKRGFMAVVSELEQRFPDPASIESEKEKKDF
                     VKLFGEYLRAENVLQNYDEFATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQMIR
                     LPAERKIQDYRSAYNDIRDWQHREKEADKKEKSTTDWDDVVFEVDLLKSQEINLDYIL
                     GLIFEHSRQNKSKDEMTVEVKRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDT
                     FFTFAQREQQREADALIKEENLNEEAARRYIRTSLKREYATENGTELNETLPKLSPLN
                     PQYKTKKQTVFQKISAFIEKFKGVG"
BASE COUNT          923 a          652 c          682 g          734 t
ORIGIN      
        1 gaactgattc aggacctgca gaatcagggc tatgaattta tttccgtaaa atcacagtcg
       61 gcgatgctgg ctaatgtccg ggaacagctt cagagcctca atggcgtggt gtttaatgac
      121 agcgaatggc ggcgtttcac ggagcagtat ctggacaacc ctggcgatgg cattctggat
      181 aagacccgta aaatccatat cgactatatc tgtgacttta tttttgacaa cgggcgtctt
      241 gagaatatct atctgataga taaaaagaat ctcatgcgca acaaagtgca gattatccgg
      301 cagtttgaac aggcaggttc tcacagtaac cgttatgacg tcactatcct ggttaatggt
      361 ttgccgctgg tgcaaatcga actgaaaaag cgtggcgtgg cgattcgtga ggctttcaac
      421 cagatacatc gttacagcaa ggagagtttt aacagcgaaa attccctgtt taagtatctg
      481 caactgttcg tcatttccaa cggtaccgat acccgttact ttgccaacac gacaaagcgc
      541 gataaaaaca gttttgactt caccatgaac tgggcgaaat cagacaacac gctgattaaa
      601 gacctcaaag attttaccgc cacctttttc cagaaacata ccctgcttaa tgttctggtg
      661 aactacagcg tttttgacag cagtcagacg ctactggtga tgcgaccgta ccagattgct
      721 gccaccgagc gcattctgtg gaaaattaag agctccttta cagcgaagaa ctggtcaaaa
      781 ccggaaagcg gtgggtatat ctggcacacc acgggttccg gtaaaacact gaccagcttt
      841 aaagccgcgc gtctggcaac agaactggac tttattgata aagtcttctt tgtggtcgac
      901 aggaaagacc tcgattacca gaccatgaag gaatatcagc gtttttcgcc agatagtgtt
      961 aacggctcgg aaaataccgc aggccttaaa cgaaatctgg ataaggacga taacaaaatt
     1021 atcgtcacca ctattcagaa acttaataac ctgatgaaag cagaaagcga cctgcctgta
     1081 tacaatcagc aagtggtgtt tatatttgat gaatgccacc gcagccagtt tggagaagcg
     1141 cagaaaaatc tgaagaagaa attcagacgc tattatcagt ttggttttac cggcacacct
     1201 attttcccgg aaaacgcctc aggttcagaa acgaccgcca gcgtatttgg tcgtgaattg
     1261 cattcgtatg tgattaccga tgcgattcgt gacgaaaaag tgctcaaatt caaggtggac
     1321 tacaacgatg tgcggccaca gtttaagtct ttagagacag aaactgacga gaaaaaactg
     1381 agtgcggctg aaaatcagca ggcgtttctt catcccatgc gtattcagga aatcacgcaa
     1441 tatatcctga ataacttccg ccagaagacc caccgcactt tcccgggttc cagaggtttt
     1501 aatgccatgc tggcagtaag cagcgtggat gctgcgaaag tctattacgc gacgtttaaa
     1561 cgcttacagg aggaagccgc taataaatct gcaaattata aaccactgcg tattgcgaca
     1621 atattctcct ttgccgccaa tgaagaacaa aatgccattg gtgaaatttc cgatgaaact
     1681 tttgataccg gcgcaatgga cagcagtgct aaagagtttc tggatgctgc aattcgtgaa
     1741 tataacgacc attttcaaat gaatttcagt actgatagca aggattttca gaactactat
     1801 cgggacctgg ctcagcgggt taaaaatcag gatgttgacc tgttaatcgt cgtggggatg
     1861 tttttaaccg gcttcgatgc tccaacactg aacacgctgt tcgtcgataa aaacctgcgt
     1921 tttcacggtc tgatgcaggc gttttcccgt accaaccgca tttatgacgc cacgaaaacc
     1981 ttcggtaaca tcgtcacatt ccgtaacctg gagcaggcaa ccatcgatgc catcacactt
     2041 tttggtgata aaaacaccag aaatgtggtg ctggaaaaga gttatgcaga gtatatggaa
     2101 ggctttacag atgctgccac tggcgaagct aaacgcggct ttatggcagt agtttcagaa
     2161 ctggaacaaa ggttccctga ccctgccagt attgaaagcg aaaaagagaa aaaagacttc
     2221 gttaaactgt ttggtgaata cctgcgtgcc gagaacgtcc tgcaaaacta tgatgaattt
     2281 gccacgctga aagccctgca acaaatcgat ctcagcgatc ctgttgcggt agaaaaattc
     2341 aaagcagaac attatgtgga tgatgaaaag ttcgctgaat tgcaaatgat tcgtctccct
     2401 gctgaacgca agattcagga ttatcgttct gcctataacg atattcgtga ctggcagcac
     2461 cgcgaaaaag aagctgacaa aaaagaaaaa tcaaccactg actgggatga cgtggttttt
     2521 gaggtcgatt tgctgaagtc tcaggaaata aacctggatt atatccttgg cctgattttc
     2581 gaacacagca gacaaaacaa aagtaaggac gaaatgaccg tagaggtcaa acgattaatt
     2641 cgttcaagtc tggggaaccg tgctaaagaa ggcctggtgg tcgattttat tcagcaaacg
     2701 aaccttgatg atttgccgga caaagccagc atcattgata cgttctttac ctttgctcaa
     2761 cgcgaacaac aacgtgaagc agatgcattg atcaaagaag aaaatcttaa tgaagaagcg
     2821 gccaggcgct atattcgcac gtcgttaaaa cgcgaatacg ccaccgaaaa tggcacagaa
     2881 ttaaacgaga cattaccaaa acttagtccg ttaaacccgc aatataaaac gaaaaaacag
     2941 accgtttttc agaagattag cgcatttatt gagaagttta aaggcgtggg g
//
LOCUS       DAZKFU020000378         2968 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZKFU020000378 DAZKFU020000000
VERSION     DAZKFU020000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.380"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            50..1186
                     /locus_tag="ACHOQQ_RS23445"
                     /old_locus_tag="pgaptmp_004689"
     CDS             50..1186
                     /locus_tag="ACHOQQ_RS23445"
                     /old_locus_tag="pgaptmp_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4472830.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            1282..>2968
                     /locus_tag="ACHOQQ_RS23450"
                     /old_locus_tag="pgaptmp_004690"
     CDS             1282..>2968
                     /locus_tag="ACHOQQ_RS23450"
                     /old_locus_tag="pgaptmp_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HGU4472831.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLT"
BASE COUNT          910 a          591 c          641 g          826 t
ORIGIN      
        1 tagtttaatt tttaaaacca tactaaaaat gtgcacaggg gattttttta tgcgaaaatt
       61 aagtatggtt gtgtgctctg tcgtttctgc attaatgctg tgtgaggcaa cacaggcagc
      121 agaaatctat aataaagacg gtaataaact ggatttttat ggtcgcgtta aagcccgtca
      181 ttacatctct gatggttcta tggatggcga cagaacattt gcacgtcttg gtttcaaagg
      241 cgaaacacaa attaatgaga gcctgactgg ctttggtcag tgggaatacc agttcctggg
      301 gtttaagaca gaaagtgaag tacgcaacga taaaaatcgt ctggcatttg caggtattcg
      361 ccacaatata ctcggttcac ttgattatgg gcgaaactat ggtgttgcct atgatgtggg
      421 ggctctgact gacgtacttc cggactttgg tggtgacagc tggacttact ctgataacta
      481 tatgaccggc agaacaaccg gagttctgac ctggcgcaat acagatttct tcgggcttgt
      541 tgatggactt actttttctg ctcaatatca gggtaaaaat gaccgtgata atttacttga
      601 agctaatggt gatggatatg gtttctccgt aagctatgac tatgaaggtt ttggaatcgt
      661 tgccgcttac actaatgccg acagaaccag tctgcaggaa aaagaaggcc gtaaacgttt
      721 catgacatcg aaagattcag aaggaaaaga agttattacg cctcgctgga ttgccggggg
      781 aaaacatgcg gagtttgcag gtgttggggt caagtataat gataatgacc tgtatctggc
      841 agctgactac tctgaaaccc gaaatatgac tccatttggt agcgcaggca tcgcagataa
      901 agcacggaat attgaagttg tggctcagta tatgtttgat ttcggcctgc gtccttcact
      961 ggcttacgtt cagtccaaag ggatggatgt taaaggtgga atagtaaact atggcgatca
     1021 gtctcttgtt gagtatattg atgttggcgc aaactacttt ctgaacaaaa acatgtcact
     1081 tcttattgat tacaaaatta accttattga tgaaagcgaa tttaccaaaa aggctggagt
     1141 tgcaaccgat aatatacttg ctgttggtat gatgtatcag ttttaacagg tatccattta
     1201 aacgatttac atagttttta tttatatcag aggcttttat tattaataaa tgttcgtgat
     1261 gcaactcaga aggaatacca tatggaacat cagttaaata aaaaactgct taactcatca
     1321 atatcactta tattagcatc tggtctgatg atgagtactg caaatgcagc agatgatgtt
     1381 aagctcaaag caacaaaaac caatgttgcc tttgcggact tcacgccgac ggaatacagc
     1441 actaaaggta agccaaatat cattgtttta acaatggatg atttagggta tggacaattg
     1501 ccttttgatg atagttcatt tgaccctaaa acgatggata accgaaatgt tgttgataca
     1561 tacaaaatag gtattgataa agcaattgaa gctgcaaaaa aatccactcc aacattagcg
     1621 gccctgatgg acgaaggtgt aaaattcact aacggctatg ttgcacacgg tgtctctggc
     1681 ccttcacgtg ccgcaatcct gacggcgcga gctcctgctc gcttcggtgt atattcaaat
     1741 tctgatgcac aggatggtat ccctctggaa gaaacattcc tgtctgaatt acttcagaat
     1801 catggttatt acactgcagc aatcggtaag tggcacttat caaaaatcag caacgtaggt
     1861 gtgcctgaag aaaaacaaac ccgtgactac cacgataact tcaccacctt ctccgcagaa
     1921 gaatggcaac ctcagaatcg tggcttcaac tacttcatgg gctatcacgc atccggcgtt
     1981 tcactttata actccccgtc attgttccgt aaccgtgaaa acattaaagc tgaagggtac
     2041 attagtgatc aattaactga tgaagcaatt ggtgtaattg accgtgcaag caaactggaa
     2101 cagccattta tgatttatct ggcatacagt gcaccacacc tgcctaacga tcagcctgcg
     2161 cctaaaaact atcaggagaa attcaacact ggtagcaaga ctgctgataa ctattatgcg
     2221 tcaatttact ccgttgacca gggggttaaa aagatcctga atacacttaa ggaaaaaggt
     2281 cagtacgaca acaccatcat tctgttcacc tctgataacg gtgctgtaat tgatggtcca
     2341 ctgccactga atggtgcgca ggctggttac aaaggccaac cgtatccggg tggtatgcat
     2401 acacctatgt tcatgtggtg gaatggcaag ctgaaaacgg gtgattatga caaactggtt
     2461 tcatcaatgg acttctatcc gaccgcttta gatgccgcag atatcaagat ccctgcttct
     2521 ctgaaactgg atggtgtatc attattgcca tatgtacaga aaggagctga taagaaatct
     2581 gcagctggcg aacctcacaa atatctgtcc tggattacct cttattctca ctggtgggac
     2641 gaagagaaca ctgcattctg gaacggatat cataaattcg tgagaaaaga gtctgatgat
     2701 tatccgcaca acccacatac agaagactta agccagtttg catacactgt aagaagcaac
     2761 gattattctc tggtttacac ctatgcagag aataaactag ctctgtacga tatcaacgat
     2821 ctgcaacaaa ctaaagatct ctctgcatcc catcctgatg tagttaagaa tatgaaggct
     2881 gttatgattg acttcatcac gtcaagtcaa aaaccgatca gtgatatcaa ccaagccaaa
     2941 tttgagaata taaaaaaatc tttaacta
//
LOCUS       DAZKFU020000379         2897 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZKFU020000379 DAZKFU020000000
VERSION     DAZKFU020000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.381"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..415
                     /locus_tag="ACHOQQ_RS23455"
                     /old_locus_tag="pgaptmp_004691"
     CDS             <1..415
                     /locus_tag="ACHOQQ_RS23455"
                     /old_locus_tag="pgaptmp_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU4472832.1"
                     /translation="TLFKNTCFYNVRVKNVYLEGAYLNNDNIVKKANNSTEKQSIDST
                     DKQANDSTVQQSIDSTDKQANDSTEKQANNSTEKQANNSTEKQANNSTDKQAKNSTEQ
                     QDSNSSNQARLKKEVNIRFSIPGLTSYQPTYIVEE"
     gene            681..1523
                     /locus_tag="ACHOQQ_RS23460"
                     /old_locus_tag="pgaptmp_004692"
     CDS             681..1523
                     /locus_tag="ACHOQQ_RS23460"
                     /old_locus_tag="pgaptmp_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU4472833.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNANSEVSILKKIKIPLQETPTNCGFEGLAYSPQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKALQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            1591..2769
                     /locus_tag="ACHOQQ_RS23465"
                     /old_locus_tag="pgaptmp_004693"
     CDS             1591..2769
                     /locus_tag="ACHOQQ_RS23465"
                     /old_locus_tag="pgaptmp_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4472834.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
BASE COUNT          765 a          695 c          655 g          782 t
ORIGIN      
        1 aacgcttttt aaaaatacct gtttttacaa tgtcagagtg aaaaatgtct atcttgaggg
       61 agcatatctg aacaatgata atatcgtgaa aaaagccaat aacagtaccg agaaacaatc
      121 cattgacagt accgataaac aagccaatga cagtacggtg caacaatcca ttgacagtac
      181 cgataaacag gccaatgaca gtaccgagaa acaagccaat aacagtaccg agaaacaagc
      241 caataacagt accgagaaac aagccaataa cagtaccgat aaacaagcca agaacagtac
      301 cgagcaacag gacagtaaca gttctaatca agcccgttta aagaaagaag taaatataag
      361 attttccatt ccgggtttaa cgtcttatca gccaacgtat atagttgaag aatagttcaa
      421 taatcaccca aacagagcag gtatggcaga ggatataaat taacatttaa acgttaaccg
      481 cctcatctat cattcatgga tgaggcgcat ttatcagtcg atgtaagctg aaaatgttct
      541 gccatccaga gacaaccgcc gccccctgcg gcgtttacta tttcatcgcc cgcgttaaga
      601 attcattaag cttcatccgt atactcctta tctgtcgcac tgaatttgac gagaaaaaag
      661 caataataag gagcaataag ttgaaaaagc gcatcacctt tatcgttttt ctttgcgcca
      721 tcgtggcggc aagcctgttt tttgtgcaat cctgcgtccg taaaagccag catgtcgcag
      781 ggtttcagaa ttatcaggcc actatcgatg gtaaagagat tacaggagta actaaaaata
      841 tctcctcact gacctggtca gcgcagagca ataccttatt tagcactatc aacaaacctg
      901 caaccatcgt tgaaatgacg accgagggcg acctgatccg caccattccg ctcgattttg
      961 ttaaggatct cgagacgatt gaatacattg gtgataataa atttgtcatc agtgatgaac
     1021 gtgattacgc catttatgtg atttcactta acgctaactc ggaagtgagc atcctcaaaa
     1081 aaattaaaat cccattgcag gaaacgccta ctaactgtgg ctttgaagga ctggcttact
     1141 cccctcagga ccataccttc tggtttttta aagagaaaaa tccgatagag gtctacaaag
     1201 taaccgggct attgcgtagc gatgagttgc atatcagcaa agataaagcg ttgcagcgtc
     1261 agtttacttt agacgatgtt tctggcgcgg aatttaaccc acagaaaaat acgttgctgg
     1321 tgttgtcaca tgagtcacgc gccctgcagg aagtgaccgt gagaggtgac gtgatcggcg
     1381 aaatgtcgtt aaccaaaggt aagtatggcc tttcacacaa catcaagcag gcggaaggga
     1441 tagcgatgga tgatagcggc aacatctata tcgttggcga accgaatctt ttttatcgct
     1501 ttacgtcgac aaaatcacga taacagtctg gcaggttctc cttttttgtt attaacttgt
     1561 taactcaaaa taatacaaac caggcagaac atgaattcga catcgcatcc cgtagaacgt
     1621 ttttctttca gcaccgcgtt attcgggatg ctggttctga ccttaggtat gggtttaggc
     1681 cgctttctct atacgccgat gctgccagtc atgctggcgg aaggcgagtt ttcctttagc
     1741 gaactctcat ggatcgccag tggtaactat gccgggtatc tggcggggag cctgctgttt
     1801 tcattcggcg cgtttcattt accctcacgc ctgcgcccgt tcctgttagc ttccgccctc
     1861 gcaaccggat tattaatcct cgcgatggcg tggctgccgc cgtttcttct ggtttttatc
     1921 attcgctttc tggcgggagt cgccagcgcc ggaatgttga ttttcggctc aacgctcatc
     1981 atgcaacata cccgccatcc ctttgtcctt gcggcgctat tttctggtgt tggcgtcggg
     2041 attgctctgg gcaatgaata tgtgctggca ggcctgcatt ttgccctctc ttcacaaacg
     2101 ttgtggcaag gtgccggagc actttctgcc attatattgc ttgctctggc gctgctcatc
     2161 ccgtcgaata aacacgttat cccgccagcg ccattggcaa aaatcgcgca acaacccatg
     2221 agctggtggt tactggcgat tctgtatggt ctggcgggtt ttggttatat catcgtcgcc
     2281 acctacctgc cgctcatggc gaaagacgcg ggccagcctg tgttgacggc tcacctctgg
     2341 acactggttg gcttgtcgat tgtcccaggt tgctttggct ggctgtgggc agccaaacgg
     2401 tggggagcat taccttgcct gaccgcgaat ttgctggtgc aggcgatctg cgtgctgtta
     2461 accctcgcca gcagctctcc tttattactc atcatcagca gtattggttt tggcggcacc
     2521 tttatgggaa cgacctcgct ggtgatgacc atcgcccgcc agcttagcgt gccgggaaat
     2581 cttaaccttt tgggctttgt gacactcatt tatggtatcg ggcaaattct tggcccggcg
     2641 ctgaccagta tgctcggcaa cggaacgtcg gcgctcgcca gtgccacact ctgcggcgca
     2701 gcggcgctat ttatcgcagc attaatctgc gggatgcaaa tattcaaatt gcatacgaat
     2761 tattcttaag taatttaacg aatttaactt ttcagtacat cacgtaaaat aattaaaaca
     2821 tcctttttct ttggaggtac tcagccaccg gcgttattag tcctggttta gcaattttac
     2881 ggctaattca ttatttc
//
LOCUS       DAZKFU020000380         2866 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZKFU020000380 DAZKFU020000000
VERSION     DAZKFU020000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.382"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1869
                     /locus_tag="ACHOQQ_RS23470"
                     /old_locus_tag="pgaptmp_004694"
     CDS             <1..1869
                     /locus_tag="ACHOQQ_RS23470"
                     /old_locus_tag="pgaptmp_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4472835.1"
                     /translation="PESLRPVTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAP
                     VLLFIDEAHTLIGAGNQAGGADAANLLKPALARGELRTIAATTWSEYKQYLERDAALE
                     RRFQMVRVDEPDDETACLMLRSLKSRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPD
                     KAVDLLDTAAARVRMSLDTVPEQLTRIRSQLASLGMEKQALLEDIAVGHQNHGERLSA
                     IEQEENVLMTARDDLEQQYARECELTGELLESRSDISRQSETLHLQQALHDIQQNQPL
                     LSVDVDVRTVAGVVADWTGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQRL
                     RAAKTGLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEPHT
                     VSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMR
                     DGEGREIDFRNTVILMTSNLGSDLLMQQLSEKPETTESELHELIRPLLRDHFQPALLA
                     RFQTVIYRPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERLYDALTSACLLPDTG
                     ARNVESLLNQQLLPVLSRQLLSHMAAKQKPQALTLTWSDEDGIVMVFDAQAEGEPS"
     gene            1866..>2866
                     /locus_tag="ACHOQQ_RS23475"
                     /old_locus_tag="pgaptmp_004695"
     CDS             1866..>2866
                     /locus_tag="ACHOQQ_RS23475"
                     /old_locus_tag="pgaptmp_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGU4472836.1"
                     /translation="MNRSLTSKAGSVVQQLSGQSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQVPMSDGTPALPIRTVFGVVESFRRIF
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPPRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYEFGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTTGDAESPETGAYFARLRHERILNAQYCVSGHSSSPYLTPGQVLETDG
                     "
BASE COUNT          625 a          764 c          864 g          613 t
ORIGIN      
        1 ccggaatcac tcagacccgt caccctgcgc acccttgacc tcggcctgct gcaggccggt
       61 gccggcgtga agggtgaatt tgaacagcgc ctgaaaaacg tgattgatgc cgtgcagcag
      121 tcaccggctc ctgtactgtt gtttatagat gaagcccata cccttatcgg ggccggtaat
      181 caggccggcg gcgcggatgc ggccaacctg ctgaagcctg cgctggcgcg gggtgaactg
      241 cgtaccattg ccgccaccac ctggtctgag tacaaacaat acctggaacg tgacgcggct
      301 ctggagcggc gttttcagat ggtcagagtg gacgagccgg atgatgagac ggcctgtctg
      361 atgctgcgct ccctgaaatc ccgttatgcg gaacatcata acgtgcatat caccgatgag
      421 gcggtacgtg ctgccgtcac actgtcgcgc cgttatctga cagaacgtca gttaccggac
      481 aaggccgttg acctgctcga tactgctgct gcccgtgtgc gtatgagcct cgacacggtg
      541 ccggaacagc tgacccgtat ccgttcacag ctcgcttccc tcggtatgga aaaacaggca
      601 ctgctggaag atattgccgt tggtcatcag aatcacggcg aacgtctgtc tgccattgag
      661 caggaagaga atgtgctgat gacagcacgt gatgacctgg agcagcagta cgcccgggag
      721 tgcgaactca ccggtgagtt acttgaaagt cgcagcgata tttcccgtca gagtgagaca
      781 ctgcatctgc agcaggcact gcacgacatt cagcagaatc agcccctgct cagtgtggat
      841 gttgacgtac gcaccgttgc cggtgtggtc gcggactgga ccggggtgcc gttatcttcc
      901 ctgatgaagg acgaacagac ggaactgctg catctggaaa aggatatcgg cagacgggtg
      961 gtcggacagg acgtggcact ggaatccatt gcgcagcgtc tgcgtgcggc gaaaacgggt
     1021 ctgacatccg gtaacggccc ccagggggtg ttcctgctgg ttgggcccag tggtgtggga
     1081 aaaaccgaaa cggcactggc gctggcagac gtgatgtacg gcggcgaaaa atcactgatt
     1141 accatcaatc tgtcggaata ccaggagccc catacggttt cccagcttaa gggttcaccg
     1201 cccgggtacg tgggatacgg tcagggcggt attctgacgg aagctgtgcg taaacgccct
     1261 tacagcgtgg tgctgctgga tgaagtggaa aaggcccacc gggatgtgct gaatctgttc
     1321 taccaggtgt ttgaccgggg ctttatgcgc gacggcgagg gacgtgaaat tgacttccgt
     1381 aatacggtca ttctgatgac ctccaacctg ggtagtgacc ttctgatgca gcaactgagc
     1441 gaaaagccgg agacaacgga atcggagctg catgagctta tccgtccact gctgcgcgac
     1501 cacttccagc cagcactgct tgcccgtttc cagaccgtga tttaccgtcc gctgacgcag
     1561 tctgccatgc gcaccattgt ggagatgaag cttgcacagg tctgcgaacg cctgcactgc
     1621 cattatgggc tgagcacatc ggttgatgaa cgtctgtacg atgccctgac atcagcctgc
     1681 ctgctgccgg atacgggcgc ccggaatgtg gagagtctgc tgaaccagca actgctgccg
     1741 gtactgagcc ggcagttgct gagccatatg gccgcgaagc agaaaccaca ggcgctgact
     1801 ctgacatgga gtgacgagga cggtattgtg atggtatttg atgcacaggc agagggagag
     1861 ccgtcatgaa ccgttccctg accagcaagg ctggcagcgt tgtacagcag ctttcagggc
     1921 aatcacggta ccgggttcat gtgcatgaat gcagccattt tctggatgtg ttgcgttaca
     1981 gcgcggttga gtccctcagc cagccctggc gctatgacgt ggttgtcacc tgctcttctg
     2041 cggatattgc ctgtgacgcg ttactgctga agccggcgtc atttactttc caggtcccga
     2101 tgtctgacgg aacaccggcc ctgccgatca ggacggtatt cggcgtggtt gagtcgtttc
     2161 gtcgtatctt tgcctcgaat gatgagaccc gttacgcact gacgattgtt ccccgtatcg
     2221 cccttctgaa ctatacgaaa gggagcgaaa tctacctcaa tcagtcagtc actgaagtgg
     2281 ttgagcaggt attacgtaaa catggtctgg aagggcagga ttttgaattc cggctctccc
     2341 gggagtatcc acctagggaa ctcatcaccc ggtggcggga aactgacctt gagtttatcc
     2401 ggcgcctgct ggcggaggtg gggatttact ggcgctatga aatggacagc cgtcttgaac
     2461 aggacatagt gattttccag gacagccagc agcagtatga atttggcatc acgctgccat
     2521 tgcgcaatca ggccgggatg agtgacagcg ggcaggagag catctgggat attcagacca
     2581 cctgtaacgt ggtgagcgga agtgtggtca cccgggacta caactaccgt gaagcgctga
     2641 aacctcagga cagtgccgaa agtattttca gtaaggaagg gatcaccacc ggagaaacgt
     2701 accattatgc tgaacccttc ctgacgacag gtgacgctga aagcccggag accggggcat
     2761 actttgcccg tctgcgtcac gaacgcattc tgaacgcgca gtattgtgtc agtgggcatt
     2821 cctccagccc ttatctgacg ccgggtcagg tacttgaaac ggacgg
//
LOCUS       DAZKFU020000381         2866 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZKFU020000381 DAZKFU020000000
VERSION     DAZKFU020000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.383"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1881
                     /locus_tag="ACHOQQ_RS23480"
                     /old_locus_tag="pgaptmp_004696"
     CDS             <1..1881
                     /locus_tag="ACHOQQ_RS23480"
                     /old_locus_tag="pgaptmp_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4472837.1"
                     /translation="PESLRPVTLRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAP
                     VLLFIDEAHTLIGAGNQAGGADAANLLKPALARGELRTIAATTWSEYRQYLERDAALE
                     RRFQMVRVDEPDDETACLMLRSLKSRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPD
                     KAVDLLDTAAARVRMSLDTVPEQLTRIRSQLASLGMEKQALLEDIAVGHQNHGERLSA
                     IEQEENVLMTARDDLEQQYARERELTGELLESRSDISRQSETHHLQQALHDIQQNQPL
                     LSVDVDVRTVAGVVADRTGVPLSSLMKDEQTELLHLEKDIGRRVVGQDVALESIAQRL
                     RAAKTGLTSGNGPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEPHT
                     VSQLKGSPPGYVGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMR
                     DGEGREIDFRNTVILMTSNLGSDLLMQLLDEQPEATEASLHELLRPILRDHFQPALLA
                     RFRTVIYRPLTQSAMRTIVEMKLAQVCERLHCHYGLSTSVDERVYDALTSACLLPDTG
                     ARNVESLLNQQLLPVLSRQLLSHMAAKQKPQALTLAWSDEDGIVLVFDAQAEGESSCA
                     VS"
     gene            1866..>2866
                     /locus_tag="ACHOQQ_RS23485"
                     /old_locus_tag="pgaptmp_004697"
     CDS             1866..>2866
                     /locus_tag="ACHOQQ_RS23485"
                     /old_locus_tag="pgaptmp_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGU4472838.1"
                     /translation="MRSFLTSKADSVVQQLSGLSRYRVHVHECSHFLDVLRYSAVESL
                     SQPWRYDVVVTCSSADIACDALLLKPASFTFQTPVSDGTPALPLRTVFGVVESFRRIS
                     ASNDETRYALTIVPRIALLNYTKGSEIYLNQSVTEVVEQVLRKHGLEGQDFEFRLSRE
                     YPSRELITRWRETDLEFIRRLLAEVGIYWRYEMDSRLEQDIVIFQDSQQQYESGITLP
                     LRNQAGMSDSGQESIWDIQTTCNVVSGSVVTRDYNYREALKPQDSAESIFSKEGITTG
                     ETYHYAEPFLTAGDAESPETGAYFARLRHERILNAQYRVSGHSSSPYLTPGQVLETDG
                     "
BASE COUNT          608 a          772 c          871 g          615 t
ORIGIN      
        1 ccggaatctc tcagacccgt caccctgcgc acccttgacc tcggcctgct gcaggccggt
       61 gccggcgtga aaggtgaatt tgaacagcgc ctgaaaaacg tgattgatgc cgtgcagcag
      121 tcaccggctc ctgtactgtt gtttatagat gaagcccata cccttatcgg ggccggtaat
      181 caggccggcg gcgcggatgc ggccaacctg ctgaagcctg cgctggcgcg gggtgaactg
      241 cgtaccattg ccgccaccac ctggtctgag tacagacaat acctggaacg tgacgcagcc
      301 ctggagcggc gttttcagat ggtcagagtg gacgagccgg atgatgagac ggcctgtctg
      361 atgctgcgct ccctgaaatc ccgttatgcg gaacatcata acgtgcatat caccgatgag
      421 gcggtacgtg ctgccgtcac actgtcgcgc cgttatctga cagaacgtca gttaccggac
      481 aaggccgttg acctgctcga tactgctgcc gcccgtgtgc gtatgagcct cgacacggtg
      541 ccggaacagc tgacccgtat ccgttcacag ctcgcttccc tcggtatgga aaaacaggca
      601 ctgctggaag atattgccgt tggtcatcag aatcacggcg aacgtctgtc tgccattgag
      661 caggaagaga acgtgctgat gacagcacgt gatgacctgg agcagcagta cgcccgggag
      721 cgcgaactca ccggtgagtt acttgaaagt cgcagcgata tttcccgtca gagtgagaca
      781 catcacctgc agcaggcact gcacgacatt cagcagaatc agcccctgct cagtgtggat
      841 gttgacgtac gcactgttgc cggtgtggtc gcggaccgga ccggggtgcc gttatcttcc
      901 ctgatgaagg acgaacagac ggaactgttg catctggaaa aggatatcgg cagacgggtg
      961 gtcggacagg acgtggcact ggagtccatt gcgcagcgtc tgcgtgcggc gaaaacgggt
     1021 ctgacatccg gtaacggccc ccagggggtg ttcctgctgg ttggtcccag tggtgtggga
     1081 aaaaccgaaa cggcactggc gctggcagac gtgatgtacg gcggcgaaaa atcactgatt
     1141 accatcaatc tgtcggaata ccaggagccc catacggttt cccagcttaa gggttcaccg
     1201 cccgggtacg tgggatacgg tcagggcggt attctgacgg aggctgtgcg taaacgccct
     1261 tacagcgtgg tgctgctgga tgaagtggaa aaggcccacc gggatgtgct gaatctgttc
     1321 taccaggtgt ttgaccgggg ctttatgcgc gacggcgagg ggcgtgaaat tgacttccgt
     1381 aatacggtca ttctgatgac ctccaacctg ggcagtgacc ttctgatgca gttgctggac
     1441 gaacaaccgg aagccactga ggccagcctg cacgaactgc tgcgcccgat actgcgcgac
     1501 cacttccagc ctgcactgct cgcccgtttc cggaccgtga tttaccgtcc gctgacgcag
     1561 tctgccatgc gcaccattgt ggagatgaag cttgcacagg tctgtgaacg cctgcactgc
     1621 cattatgggc tgagcacatc ggttgatgaa cgtgtgtacg atgccctgac atccgcctgc
     1681 ctgctgccgg atacgggtgc ccggaatgtg gagagtctgc tgaaccagca gctgctgccg
     1741 gtactgagcc ggcagttgct gagccatatg gccgcgaagc agaaaccgca ggcgctgact
     1801 ctggcatgga gtgacgagga tggcattgtt ctggtatttg atgcacaggc agagggagag
     1861 tcgtcatgcg cagtttcctg accagcaagg ctgacagcgt tgtacagcag ctttcagggc
     1921 tatcacggta ccgggttcat gtgcatgaat gcagccattt tctggatgtg ttgcgttaca
     1981 gcgcggttga gtccctcagc cagccctggc gctatgacgt ggttgtcacc tgctcttctg
     2041 cggatattgc ctgtgacgcg ttactgctga agccggcgtc atttactttc cagaccccgg
     2101 tgtctgacgg aacaccggcc ctgccgctca gaacggtatt cggcgtggtt gagtcgtttc
     2161 gtcgcatttc tgcctcgaat gatgagaccc gttacgcact gacgatagtt ccccgtattg
     2221 cccttctgaa ctatacgaaa gggagcgaaa tctacctcaa tcagtcagtc actgaagtgg
     2281 ttgagcaggt attgcgtaaa catggtctgg aagggcagga ttttgagttc cggctctccc
     2341 gggagtatcc atcccgggag ctcatcaccc ggtggcggga aactgacctt gagtttatcc
     2401 ggcgcctgct ggcggaggtg gggatttact ggcgttatga aatggacagc cgtcttgagc
     2461 aggacatagt gattttccag gacagccagc agcagtatga atctggcatc acgctgccat
     2521 tgcgcaatca ggccgggatg agtgacagcg gacaggagag catctgggat attcagacca
     2581 cctgtaacgt ggtgagcgga agtgtggtca cccgggacta caactaccgt gaagcgctga
     2641 aacctcagga cagtgccgaa agtattttca gtaaggaagg gatcaccacc ggagaaacgt
     2701 accattatgc tgaacccttc ctgacggcag gtgacgctga aagcccggag accggggcat
     2761 actttgcccg tctgcgtcat gaacgtattc tgaatgcgca gtatcgtgtc agcgggcatt
     2821 cttccagccc ttatctgacg ccgggtcagg tacttgaaac ggacgg
//
LOCUS       DAZKFU020000382         2858 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZKFU020000382 DAZKFU020000000
VERSION     DAZKFU020000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.384"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>2858)
                     /locus_tag="ACHOQQ_RS23490"
                     /old_locus_tag="pgaptmp_004698"
     CDS             complement(<1..>2858)
                     /locus_tag="ACHOQQ_RS23490"
                     /old_locus_tag="pgaptmp_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4472839.1"
                     /translation="IAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTI
                     TSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQ
                     YLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALVGV
                     QVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRKAW
                     DVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKD
                     RHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLI
                     KTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDRE
                     ITLPSSGTTLISLVDGEGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQ
                     RLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTA
                     EVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQLA
                     LGNYSLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDANDK
                     WNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMF
                     VAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLN
                     NVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYA"
BASE COUNT          558 a          899 c          735 g          666 t
ORIGIN      
        1 ctggcataac tgttcgtgcg ggggaaagac ttgctgaccg ttttgacaat atcaccttca
       61 atctggttgg ctgacagttt ccccttaatc tgacagttct cattaatcgt gacgttgttg
      121 agcgtccctg agttcgcatt cacactgcca ctgatatccg catttttagc ggtcagcttt
      181 ccgtctgatg tcagggaaaa taccggagga ctgccaccgc tggtaatggt gggtgccgtc
      241 aggcgtttca ggaacacgtc gttcatgaat atctggttgc cctgcgccac aaacatcggc
      301 gtttcattcc cgtttgccgg gtcaataaac gcgatacggt tagcggcaac cagaaactgg
      361 ctcagcttgc cttcctccgt atcctccata ctgaggccaa tacccgcgac ataatgtttg
      421 ccgtctttgg tctgctcaat tttgacagcc cacatggcat tccatttatc gttggcatcc
      481 ttccactctt tcgaaaactc atccagtctg ctggcgttat cctccgtcag atctactttt
      541 tccagcagct ctttaccgag atgggattcg gttatcttgc ctttgaaaaa atccaggtaa
      601 ccttccgcat catcgctcgc ccgaccgacg gcctccacaa atgccgattt gccaacggtg
      661 ttcacactgc ggatataaaa gtaataatca tggcccggtt tgatattgat actggcggct
      721 atccagtaca gccccgtgcc aaggtagcgg gctgtggttt caacctgcct gatatcggta
      781 atccgcgttt ccgagaacca gaactcaaac tgtacagtcg gatcataaac cgcaagatgc
      841 ggcgtggcgg ttatctgaaa atagcccggc gtcagctcaa tccgcgatgg cgctgccggt
      901 gcggcaatcc ggaacgatac cgacgccgga tcgccctgct gcccccaggc atttaccgcc
      961 cggactgtca gactgtaatt tcccagagcc agctgcctga agcggtatgt ggtttccgtc
     1021 gtccgggccg tgctgaccag ccgctcactg ccgtcatccg ctgccacggt caggcgaagc
     1081 ataaagctca cgcccttcac caccttcggc gtatcccagc gcgccagcac ctggtattcc
     1141 ccgctgtctg cggtgacttc tgcggtcagg tgttgcactg ctggcggagt gacaccattc
     1201 accgtgccgc tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc ctctttctcc
     1261 ggtacatgct gcacggcagt gatggcatac gtgccgtcgt cgttctcacg gatactcacg
     1321 cagcggaaca ggcgctggcg caacgtcggc aacttcagcc cccacacgct gtattcagca
     1381 acgccgtcgg gaacacggct cactttcacc ttcacgccgt cggtgacgga ctggacctcc
     1441 acgctgaccg gattaccctc accgtcaacc aggcttatca gcgtggtgcc ggaggatggc
     1501 agcgtgattt cacggtcgag cgtcagcgtc cgggtctggc tgtttaccgc cagcacgcgc
     1561 ccgccggtgc tgataccggc atagtcatca tcacagattt caatgacatc gcccggcaca
     1621 tggcgaagcc cttctgcgcc cacgctgaag tccacggtct gcgtttccag cagttctgtt
     1681 ttaatcagcc acagcccggc gcggtgtgcc tgcccccggc tggtacagcc aaaagcatcc
     1741 atcttcgtga cgttacgacc gtaacgggca atggcctgcg tatcctccac aagctctgtc
     1801 gccgtctccc agccgttgtt cgggtcaatc cagttcacct caacggcatt atggcggtct
     1861 ttcagggcgc tgaagctgta gcggaacggc gcgccatcat ccggcatcac cacattactg
     1921 cggttatagg tccacacctt atccgatggc cggtcctgca caaacgtcag cgtctgcccg
     1981 ttccataccg gcatacagcg catcgccgag cagaaatcac tgagcacatc ccacgccttg
     2041 cgctgcgtgg tcaggtacgc attacaggta atacgcggct ccgtgccacc aaagccatcc
     2101 ggcaccgact ggtcgcaatt ctggccgatg acatacagcg cccatttatc cacatccgcc
     2161 gcaccaagac gtttccccat gccgtagcgc ggatgggtca gcatatccca cagacaccag
     2221 gccatgttgt tgctgtatgc tggcttaaac gttccgtccc agataccgct gtattgccgc
     2281 gtctgcgggt tatagttcga cggcacctgc agaatgcgtc cgcgaagatg ataattacgg
     2341 ctcacctgct ggctgccgaa ctgctccgag tccacctgca cgccgaccag tgccgtgttc
     2401 gggtagcact gtttcacatc gatgatttcg gtgtatgacg accagagcgt tttgttctgc
     2461 agctggtctg tggtactgtc cggcgtcatc ctgcgcatcc ggatattaaa cgggcgcggc
     2521 ggcaggttat ccaccaccac cgaggccaga tactgcgagg tggttttgcc cttaatggtg
     2581 atgtcttttt ccgtcaccca gccaccatta cgctgtatct gaaccagcag gcgaacttcc
     2641 gacggattcc ggtccccctt tgaggtggtt tccaccagtg cctgcacacc gaaggtaaag
     2701 cgcagacggt cgatgtttgc agacgtgatg gtccgggtga tcggcgtgtc gtatttcact
     2761 tccgtaccca gcaccgtctc ggatccggag gattcaaatc cctccggcgg tgtctgctcc
     2821 tgctcaccgg cccggaacac caccgtgaca ccggcgat
//
LOCUS       DAZKFU020000383         2858 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZKFU020000383 DAZKFU020000000
VERSION     DAZKFU020000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.385"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>2858)
                     /locus_tag="ACHOQQ_RS23495"
                     /old_locus_tag="pgaptmp_004699"
     CDS             complement(<1..>2858)
                     /locus_tag="ACHOQQ_RS23495"
                     /old_locus_tag="pgaptmp_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4472840.1"
                     /translation="ISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTI
                     TSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQ
                     YLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALFGV
                     QVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRKAW
                     DVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKD
                     RHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLI
                     KTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLDRE
                     ITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTLRQ
                     RLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTA
                     EVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLA
                     LGNYRLTVRAANAWGQQGDPASASFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVDLTEDNASKLEQFSKEWKDANDK
                     WNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMF
                     VAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGTLN
                     NVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYA"
BASE COUNT          547 a          896 c          746 g          669 t
ORIGIN      
        1 ctggcataag tgctcgtgcg ggggaaagac ttgctgaccg ttttgacaat atcgccttca
       61 atctggttgg ctgacagttt ccccttaatc tgacagttct cattaatcgt gacgttgttg
      121 agcgtcccgg agttcgcatt cacactgcca ctgatatccg catttttagc ggtcagcttt
      181 ccgtccggtg tcagggaaaa tgccggtgga tttccaccgc tggtaatggt gggggccgtc
      241 aggcgcttca ggaacacatc gttcatgaat atctggttgc cctgcgccac aaacatcggc
      301 gtttcattcc cgtttgccgg gtcaataaac gcgatacgat tggcggcaac cagaaactgg
      361 ctcagtttgc cttcctccgt gtcctccatg ctgaggccaa tacccgcgac ataatgtttg
      421 ccgtctttgg tctgctcaat tttgacgccc cacatggcat tccacttatc gttagcatcc
      481 ttccactctt tcgaaaactg ctccagtttg ctggcgttat cctccgtcag gtcgactttt
      541 tccagcagct ccttgccgag atgggattcg gttatctggc ctttgaaaaa atccaggtaa
      601 ccttccgcat catcgctcgc ccgaccgacg gcctccacaa atgccgattt gccaacggtg
      661 ttcacactgc ggatataaaa gtaataatca tggcccggtt tgatattgat actggcggct
      721 atccagtaca gcgccgtacc aagatagcgg gctgtggttt caacctgcct gatatccgca
      781 atccgctttt ccgaaaacca gaactcaaac tgtaccgtcg gatcataaac ggcaagatgc
      841 ggcgtggcgg ttatctgaaa atagcctggc gtcagctcaa tccgcgacgg cgctgccggt
      901 gcggcgatcc ggaacgatgc cgatgcagga tcgccctgct gtccccaggc atttgccgcc
      961 cggactgtca gcctgtagtt tcccagcgcc agctgcgtga agcggtatgt ggtttccgtc
     1021 gtccgggccg tgctgaccag ccgctcactg ccgtcatccg ctgccacggt caggcgaagc
     1081 ataaagctca cacccttcac caccttcggc gtgtcccagc gcgccagcac ctggtattca
     1141 ccgctgtctg cggtgacttc tgcggtcagg tgctgcactg ctggcggcgt gacaccgttt
     1201 accgttccgc tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc ttctttctcc
     1261 ggtacatgct gcacggcagt gatggcatac gtgccgtcat cgttctcacg gatactcaca
     1321 cagcggaaca ggcgctggcg caacgtcggc agcttcagcc cccacacgct gtatccggca
     1381 acgccgtcag gaacacggct cactttcacc ttcacgccgt cggtgacgga ctgaacctcc
     1441 acgctgaccg gattgccact tccgtcaacc aggcttatca gcgtggtacc ggaggatggc
     1501 agcgtgattt cacggtcgag cgtcagcgtc cgggtctggc tgttcaccgc cagcacgcgc
     1561 ccgccgatgc tgatacccgc atagtcatca tcgcagattt caatgacatc ccccggtaca
     1621 tggcgaagcc cttccgcacc cacgctgaag tccacggtct gcgtttccag cagttccgtt
     1681 ttaatcagcc acagcccggc gcggtgcgcc tgcccccggc tggtacagcc aaaggcatcc
     1741 atcttcgtga cgttacgacc gtaacgggca atggcctgcg tatcttcaac aagctctgtc
     1801 gccgtctccc agccgttgtt cgggtcaatc cagttcacct caacggcatt atggcggtcc
     1861 ttcagggcgc tgaagctgta gcggaacggc gcgccatcat ccggcatcac cacattactg
     1921 cggttatagg tccacacctt atctgatggt cggtcctgca cgaacgtcag cgtctgcccg
     1981 ttccataccg gcatacagcg catcgccgag cagaaatcac tgagcacatc ccacgccttg
     2041 cgctgtgtgg tcaggtacgc attacaggtg atgcgcggct ccgtgccgcc aaagccgtcc
     2101 ggcaccgact ggtcgcagta ctggccgatg acatacagcg cccatttatc cacatccgct
     2161 gcaccaagac gtttccccat gccgtagcgc ggatgggtca gcatatccca cagacaccag
     2221 gccatgttgt tgctgtatgc cggtttaaac gttccgtccc agataccgct gtattgccgc
     2281 gtctgcgggt tataattcga cggcacctgc agaatacgcc cgcgcagatg ataattacgg
     2341 ctcacctgct ggctgccgaa ctgctccgag tccacctgca cgccgaacag tgccgtgttc
     2401 gggtagcact gtttcacatc gataatttcg gtgtatgacg accagagcgt tttgttctgc
     2461 agctggtctg tggtgctgtc cggcgtcatc ctgcgcatcc ggatactgaa cgggcgcggc
     2521 ggcaggttat ccaccaccac cgaggccaga tactgtgaag tggttttacc cttaatggtg
     2581 atgtcttttt ccgtcaccca gccaccgtta cgttgtatct gaaccagcag gcggacttcc
     2641 gatggattcc tgtccccctt tgaggtggtt tccaccagtg cctgcacgcc gaaagtaaaa
     2701 cgcagacggt caatgtttgc cgacgtgatg gtccgggtga tcggcgtgtc atatttcact
     2761 tctgtaccga gcaccgtctc ggagccggag gattcaaatc cctccggcgg tgtctgctcc
     2821 tgctcaccgg cccggaacac caccgtgacg ccggatat
//
LOCUS       DAZKFU020000384         2851 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZKFU020000384 DAZKFU020000000
VERSION     DAZKFU020000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.386"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..416)
                     /locus_tag="ACHOQQ_RS23500"
                     /old_locus_tag="pgaptmp_004700"
     CDS             complement(<1..416)
                     /locus_tag="ACHOQQ_RS23500"
                     /old_locus_tag="pgaptmp_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HGU4472841.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLE"
     gene            732..1916
                     /locus_tag="ACHOQQ_RS23505"
                     /old_locus_tag="pgaptmp_004701"
     CDS             732..1916
                     /locus_tag="ACHOQQ_RS23505"
                     /old_locus_tag="pgaptmp_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4472842.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     VS"
     gene            2245..>2851
                     /locus_tag="ACHOQQ_RS23510"
                     /old_locus_tag="pgaptmp_004702"
     CDS             2245..>2851
                     /locus_tag="ACHOQQ_RS23510"
                     /old_locus_tag="pgaptmp_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4472843.1"
                     /translation="MNDRLRFEVNDNQGCFVFPDAWFGSLLDEFDELIDAYDRDEISE
                     TSYINKLRRLARQEPDFIDVHAYLACAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAALLTDKILAYNPEDNQGARWLLGPEL
                     LRTGDHEQARHVLKAHADEFSPYWYELGLLHFLNRPLETPDS"
BASE COUNT          799 a          713 c          656 g          683 t
ORIGIN      
        1 atttccagca tgacagaacg tgcgccctgt gaatccacca caatgacgtc ataggtctga
       61 aaaagcggat gctgaagcac attacgcaga cgaaggcgac catccggcgc atgaagcatg
      121 gccgttttca gttgctcatg ggggtcgttg gagacgatga ggtcaagccc cctgacagcc
      181 gtacgggaaa tgatgttgtc aggatgactg aggtcagccg tgcgcatcag taattcaaaa
      241 agaccggccg gcgcttcata cgtcagcgga aagatattgc ttgccgtggg ctgtgcatgg
      301 tctccgtcta tcagcagagt acggaggccg gcatctgcca gaaaaccggc aagattagca
      361 gactgggttg attttccttc gccaccttta gtggatataa ccggtaaaat tttcatatag
      421 cacactcttt aaagtatgca accaatcact tacactacgg aaaccagcca gaatattcac
      481 ttcactacct gaatactcgc aacacgggga ttgaaaatcc ccgtgtcctt ggttcgattc
      541 cgagtccggg caccactttc acttccagat tcatctcccg aactccacga aactcaagta
      601 taatcacatc gttacaagca atctgaacat cctgaactcc accgaactct actgaactct
      661 ttctcaatca agctttcatt gggggcaccg gtgggggctc attatgttca gtcagtttca
      721 ggagccccca tatggcactt acagccagac aggtcgaaac agccagacct aaagaaaaag
      781 actataaact ctctgacgaa cgtggtttat atctgctggt aaaaaccacg ggagcccgct
      841 actggcggct taaataccgg atagcaggaa aagagaaaaa actggccctc ggcgtctatc
      901 ccgacgtctc ccttgctgag gccagaatca aacgtgacga tgcccgaaaa atcatctccg
      961 aaggtggtga cccgggcgaa aagaagcgaa aggaaaaact cactcagaaa atctctgcca
     1021 ccaatacgtt ccatgccctc gctacggaat ggcaccagca taaatctttg tcatggtctg
     1081 aaagttacgc cagaagcgta ctggaagcgc tggataaaga tattttcccg tatctgggca
     1141 aacgaagcgt tacggatatc ctcccgctgg aaatgctgga aattctgcgc cgcatagaaa
     1201 aacgtggctc gctggaaaaa ctgcgtaagg tacgtcaata ctgtaatcag atttttcgtt
     1261 atgccatcgc caccggacga gccactgtca atccggcatc tgaactgacc agtacgctgg
     1321 cggcgccaaa agctgcacac ttcccccacc tgagagcaga tgagctccct gtttttctca
     1381 ggaagctcgc tgaatatcat ggcagtcctg ttacccgcat ggcgacaaat ctgctgcttc
     1441 tgacaggcct cagaacgatt gagctacggt ccgctgaatg gtcagaaatt gattttgata
     1501 atgccctgtg gacaatccct gaaagccgca tgaaaatgcg acgtaaacat gtcgtaccac
     1561 tgtcacgaca ggtcactgac attctgcaac aactcaaaac tttctccgga caataccggc
     1621 tggttttccc gggacgttgt gatatcaaca agccaatgag cgaagccagc atcaatatgg
     1681 tgctcaaacg tatcggttac gatggcagag caaccggtca tggttttcgt cacaccatga
     1741 gtaccattct gcacgaacag gggtttaatt ctgcctggat tgaaatgcag ttagctcatg
     1801 tggataaaaa ttccatcagg ggtacctata atcatgccct gtatattgat ggtcgccgtg
     1861 aaatgatgca gtggtatgct gactacattg attcgctttc tgagcaggta agttaagctg
     1921 agaacacgtt attctgattt tcactcactt cacgattatt cctccggcct gcaggttcgg
     1981 aggattctta tttttatgtt ttattttcag ataaagtttt tgacaaaacg tttattctgc
     2041 atcagcaaaa ttatcacaaa acaacgcaat cacagacaaa ttaacatttt tcagacgggt
     2101 atattgagtg cccacaatgt aagacaggca ggcatccggt gacatggtaa aatattaatc
     2161 cagggcaaaa acaaggcttg atacacaaca tcatgacttt tatgctctca gactatgcca
     2221 ccacagattt tacaggctca gaaaatgaat gacagattac gctttgaagt gaacgataac
     2281 cagggctgtt tcgtgttccc ggacgcctgg tttggctcgt tgctggatga gtttgacgaa
     2341 ctaattgatg catacgacag agatgaaatc agcgaaacaa gttatatcaa caaactcaga
     2401 cgccttgccc gacaggagcc tgactttatt gatgtacacg cttacctggc atgcgcattt
     2461 ctggagcaga atgccccgcg taaggcactg aacgctgctc ttaaaggact ggcagcaggt
     2521 aatcgtctca tcccggaaag tttcagtggt gaaatcatct ggatgcatcc cgaaaaccgg
     2581 ccttatctgc gcgcgcttta tgcagcaatt ctcgcaaatg tgcacttaca gcgtcatcag
     2641 gatgcagctc tgctcactga taaaatactg gcatacaatc ctgaagacaa tcagggagca
     2701 cgctggctac tgggaccgga actgttacgt actggcgacc atgaacaggc acgccatgta
     2761 ctgaaagcgc atgcagatga attttcacct tactggtatg aactcggcct tctgcatttt
     2821 ctgaaccggc cccttgaaac gccagacagt g
//
LOCUS       DAZKFU020000385         2837 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZKFU020000385 DAZKFU020000000
VERSION     DAZKFU020000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.387"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(161..1732)
                     /locus_tag="ACHOQQ_RS23515"
                     /old_locus_tag="pgaptmp_004703"
     CDS             complement(161..1732)
                     /locus_tag="ACHOQQ_RS23515"
                     /old_locus_tag="pgaptmp_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGU4472844.1"
                     /translation="METSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPALPS
                     ALRQSSARKPLPTTLPRETRVIRPEEECCPACGGELSPLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     DVELSRATLGRWTGAVAELLEPLYDILRQYVLMPGKVHADDIPVPVQEPGSGKTRTAW
                     LWVYVRDDRNAGSEMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRVLYESG
                     RITEAACMAHARRKIHDVHARVPTDITTEALQRIGELYAIEAEVRGCTAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWDGLNVYCGNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLTSQ"
     gene            complement(1752..2099)
                     /gene="tnpB"
                     /locus_tag="ACHOQQ_RS23520"
                     /old_locus_tag="pgaptmp_004704"
     CDS             complement(1752..2099)
                     /gene="tnpB"
                     /locus_tag="ACHOQQ_RS23520"
                     /old_locus_tag="pgaptmp_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4472845.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKIHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(2099..2746)
                     /gene="tnpA"
                     /locus_tag="ACHOQQ_RS23525"
                     /old_locus_tag="pgaptmp_004705"
     CDS             complement(2099..2746)
                     /gene="tnpA"
                     /locus_tag="ACHOQQ_RS23525"
                     /old_locus_tag="pgaptmp_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HGU4472846.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIF
                     SLVRRFHQVFTDWPLSGEYSCGQLARALFPGRYPSTPTVTQPVKAEKPRRNRFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPE
                     PDNKIPDPVQVEQINTPTDSLCCELVLPAGTLRLKGELTPALLQTLIREMKGSSH"
BASE COUNT          598 a          792 c          763 g          684 t
ORIGIN      
        1 ctttatgacc tttgatggtc gtcggatata tcatattttg ctggtgtcgg tctgaccgat
       61 cttccgtctc actttcctgc gcaattcatt tcagtaggcc acttcagaca aacttttctc
      121 tacaacatac gtaagcggct cgccagaacc gtattgatat ttactgagag gtcagatcaa
      181 ctttccaggg caacagatcg cgtacccggt ttgccggcca gtcctggata tgctcaatga
      241 cgtaacgcag ccatttttct ggttccacat tgttcagacg gcatgtgccg atcagcgagt
      301 acaacaccgc cgcatgctcg ccaccgctgt cggaacccgc gaagagccag tttttccggc
      361 ctacggccac tccccgtaag gcgttctctg cgatgttgtt gtcgatttcc acccagccat
      421 taccgcagta cacgttcagg ccatcccact gtttcagcag gtatgcgaac gcttttgccg
      481 tatccgagtg acgcgacagt gttttcatct gagtctgtat ccagtcatac agtgactgca
      541 tcagtggcgc agctctggct tttcttgccg ccagacgctg ttctgctgta catccccgga
      601 cttctgcctc tatggcatac agttcaccga tacgctgcag ggcttccgtg gtgatgtcgg
      661 tgggcactct tgcatgcaca tcgtggattt ttctccgggc atgagccata cacgcggctt
      721 ccgttattct gccggattcg tataacaccc ggtaaccacc gtaagcatcg gcctgaagca
      781 caccgctgta accggccaga tgattttgtg gatggatacc tttccggtcc ggtgagtacg
      841 cgaaccagac cgccgggggc atttccgaac cggcgttacg gtcatcacgg acgtagaccc
      901 acagccaggc ggtccgggtt ttaccactgc ccggctcctg gaccggaacg gggatatcat
      961 cggcatggac tttaccgggc atcagcacat actggcgcag gatgtcatac agcggctcca
     1021 gcagttcagc aacggcaccg gtccagcgac ccagcgtggc acggctcagc tccacgtcct
     1081 ggcgacggta tatttctgac tggcggtata acggcagatg gtctgcatac ttcccggtga
     1141 caacatgggc cagaagcccc gctccggcat aactgcgtgc aatgggtttt gaaggcaccg
     1201 gtgcctgcac aatatggtcg caccgacaac aggccagttt cggacgttgt gtttcgataa
     1261 ccttaaaggc gctgctgata agctccagct gctctgacac atcacatccc agtggactga
     1321 gttcaccacc acaggccgga cagcattcct cttccggccg gatgacccgg gtttcacggg
     1381 gaagtgtggt cggtaacggt ttacgggctg aagactggcg cagggcggat ggcagtgccg
     1441 ggtcatattg ttcacccagc gtttccgcca tttcttcctg aagtgcgctg attcgctcct
     1501 gtgcttcctg tatctgccgt tcggtttttg cacgaagttt ttctgagctt ttaccgaact
     1561 gcatacgctg cagtttcgca accagcgcct tcagccggtt gatttcagaa gcataagccg
     1621 ccaccctctg tgagagcagg cggttgtatt cggccatctg gcggatggtg tcctgttgcg
     1681 tctgcaacag tgcccgcagg cgggcgttct catgagcaag tgaggtctcc atatcctcac
     1741 tttacaacgg gttatatgcg gattccagcg cgttccgttc gttttggatg tttccagtcg
     1801 atgccttcaa gaagcatgga caactgagcc ggagtaaggt gaatctttcc atcgcgggtg
     1861 accggccaga cgaagcggcc ccgctccagg cgtttggtga agaggcacag tccgtcgctg
     1921 tcagcccaca acacttttat ctggtcaccc cggcgtccgc ggaagatgaa cagatgcccg
     1981 gagaacgggt catccttcag gacgttctga acttttgatg ccaggccgtt aaaaccattt
     2041 cgcatgtcgg tgataccggc aaccagccag atacgcgaac cggcagggag agatatcatc
     2101 agtggctgct ccctttcatt tcgcggataa gtgtctgtaa taacgccggt gtcagttcac
     2161 ctttcagcct gagagttccg gccggcagaa ccagctcgca gcacagactg tcggtcggtg
     2221 tatttatctg ctcaacctgc acggggtccg ggattttatt atccggctcc ggagttaacg
     2281 tcacgggaag tagcgatgtt tcatgttcgc cgggcggctg caggccgccg ttacgccaga
     2341 gatgccgcca gttaaagagc aggttatcgt tgattccgtt ctcacgagct atttgtgcga
     2401 cacaggcacc gggtaaaaga gtctgctgaa caagtctcca tttaaactcc ggtgaaaatc
     2461 ggttccggcg gggtttctct gctttcacag gctgagtcac ggtcggggtt gaaggatatc
     2521 gccccgggaa aagagcccgg gccagttgcc cgcaggaata ttcaccggaa agaggccagt
     2581 cagtgaatac ctggtgaaat cgcctcacca gagaaaaaat ggcggttttg ctgacaccag
     2641 tctgcgcaga aatgcgcggg tacgacagtg actcgtcaaa tctcagctgt agcacacgca
     2701 ggatcaattc cggattatct gccagtaccg cctttcgtgc attcatctta aatgtccttt
     2761 tactgcgaaa atggacatta gtatcggaaa caggaaaagg aggcgaaaga cggtttaaat
     2821 gatgcgctta ccaacat
//
LOCUS       DAZKFU020000386         2836 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZKFU020000386 DAZKFU020000000
VERSION     DAZKFU020000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.388"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..126
                     /locus_tag="ACHOQQ_RS23530"
                     /old_locus_tag="pgaptmp_004706"
     CDS             10..126
                     /locus_tag="ACHOQQ_RS23530"
                     /old_locus_tag="pgaptmp_004706"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472847.1"
                     /translation="MSEKTFSTHKQNLIKRLRVHNEVELLYKGMLLVRKGRL"
     gene            201..389
                     /locus_tag="ACHOQQ_RS23535"
                     /old_locus_tag="pgaptmp_004707"
     CDS             201..389
                     /locus_tag="ACHOQQ_RS23535"
                     /old_locus_tag="pgaptmp_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU4472848.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            533..1378
                     /gene="mscS"
                     /locus_tag="ACHOQQ_RS23540"
                     /old_locus_tag="pgaptmp_004708"
     CDS             533..1378
                     /gene="mscS"
                     /locus_tag="ACHOQQ_RS23540"
                     /old_locus_tag="pgaptmp_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU4472849.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIGGIAARMISGVLNNLMLGRNI
                     DATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLA
                     AGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIVN
                     FSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMNF
                     VVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRS
                     VSSRQ"
     gene            1504..2724
                     /locus_tag="ACHOQQ_RS23545"
                     /old_locus_tag="pgaptmp_004709"
     CDS             1504..2724
                     /locus_tag="ACHOQQ_RS23545"
                     /old_locus_tag="pgaptmp_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HGU4472850.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
BASE COUNT          713 a          527 c          775 g          821 t
ORIGIN      
        1 tatcttggca tgtcagagaa aacattctcc actcataagc agaatctgat aaagaggtta
       61 cgggtacata atgaagttga gttgttgtac aaggggatgc ttctggtcag aaaagggcgg
      121 ttgtgaagca gggcagctta tttccggtaa tgttaaacgc agcctctgat tcgttaattt
      181 cataatcata aggagttttt atgcgtctgt tgattgcttt tcttcttccc tggctgactt
      241 ttttcaccat cggccggcct ctgacgggga ttttttgtct ggtgttacag ataacgctgc
      301 tgggctggat accggcaaca atatgggccg tgtatgcgct ttcgcagtat aagacggaac
      361 agaaaatccg cgaagccacg gggaaatgat gactgcagaa gagttggttg tcataccggt
      421 gtatatgatg cgggataaag gcccggagat tcctggaaat aaaatgttcc ggggaatata
      481 aaacagggaa aaaccggatg tgatggacgc ttctggcgag gcggtttcat gaattgtccg
      541 taatcaggat atgttgcccg gttacgccgc gaatcttgtg gcagcagtgg ttattattat
      601 cgggggtatc gctgcacgga tgatttctgg tgtgctgaat aatctgatgc tgggcaggaa
      661 tattgatgcc acagtggcgc tgttcctttc cgcgctgata cgtcatgcca ttatagcctt
      721 tacgctgatt gctgccctgg gacgtgtggg ggttcagaca gcttccgtaa ttgcagtcct
      781 gggggctgca ggtcttgctg ttggacttgc cctgcagggg tcattgtcaa accttgcagc
      841 tggtattctt ctcgtgatgt tccgtccttt cagggctggt gaatatgctg acctgggtgg
      901 tgttgccggg actgtgcaga atgtacatat attctcaacc acgatgcgta cactggatgg
      961 taaaattgtt gtcattccga acgggaagat tattgccgga gagattgtta atttctccag
     1021 ggagcctgaa cgacgaaatg agtttatcat cagtgtttca tatgatgcag atattgactg
     1081 tgtgaaacaa gtcctgacag atattgtgat gtcagaagag agggttctga agaacaggga
     1141 aatcactgtt cgcctgaatg agcctgggga gtcatccatg aattttgtag tacgggtatg
     1201 gagcaggcgt gatgaccttc agtctgtata ctgggatgta ctggagagaa ttaagcggga
     1261 gtttgattca gaagggataa gttttccata tccacaaatg gatatccatc ttgttcgctc
     1321 cgggaagcag gcagagtcgg taacccataa cagtcgctca gtcagttcca gacagtaaaa
     1381 tgaaaagcgc attttatttg tgaaagatga acagcctgaa tctgtcaggc tgttttcatt
     1441 gtgtttttat ggtcattttt ggtatatgta ctgtatataa tgtcatctgt acaaggatat
     1501 aaaatgccaa agaaaataat gacagggatt gatgtttttg acgctgccat ggagcgtatt
     1561 gaatgggtgt ttgatacgtt ttcatcagtt tgtctttctt tttccggcgg aaaagactcc
     1621 actgtcctgt ttcacctgat ggcgaatatt gcccgccgaa aaaaacgccg tttttctgta
     1681 ctgtttgttg actgggaggc tcagtatcag tgtaccattg agcatgtcac caggttgagg
     1741 gaaatgtacg atgatatcac agatactttt ttctgggtgg cgctgcctct gacgaccgtt
     1801 aatggagtgt ctcaggtaca gcctgagtgg gtatgttggg aggacggggc tgaatgggtt
     1861 cgccagccac cagaagatgc cataaccaga gcgtcttttt ttccttttta tcagaatgcg
     1921 atgacatttg aagagtttat tccggcattt aatcattggt ttgcccggga aaaagggatg
     1981 gttgctgcga ttcttactgg tgttcgcgct gatgagtcac tgaatcgttt tatgggactg
     2041 gtatccggac gtaagctccg ttatgcggag gataaaccct ggaccacggc atcggctgag
     2101 ggattttact acaccatgta tcctttgtac gactggaaaa caagagatgt atggatttac
     2161 catgcgagaa cacgtgctgc ctataactct ctttatgacc tgatgtacag ggctggagtt
     2221 cctcttcgca atatgcgggt gtgtgagcct tttggaccgg agcaacgtaa ggggttgtgg
     2281 ctttatcatg tactggagcc ggatacatgg ggcagaatgt gtgctcgcgt atcaggtgct
     2341 gtagggggag cgctctatgc aaatgaaagt ggtgcttatt ttgctttgag gaaacagata
     2401 acaaaaccag ttcaccatac atggcagagt tatgcaatgt ttctgctgga gtcaatgcct
     2461 gagaaaacgg cggagcatta caagaataag attgctgttt atcttcggtg gtggcagtct
     2521 catggatggc cggatggcat ccctgatgag caggataatg accagggatg taaagatatt
     2581 ccctcctggc gtcgcatctg taagacgtta ctgaaaaatg atttctggtg tcggatgctg
     2641 gcgtttagtc cgaataagcc ccgacattat gagcgctacc tggccagaat gcgtgaaaaa
     2701 aggaaagagt ggggaatatt gtaaattata tttaaacatc agaaatatga cggttaatcc
     2761 aggatagtag ctaaaaaaat aagattcctt gctgatttat ccctgtttga tatgtaaaat
     2821 ctgaggggct tataag
//
LOCUS       DAZKFU020000387         2824 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZKFU020000387 DAZKFU020000000
VERSION     DAZKFU020000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.389"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(56..132)
                     /locus_tag="ACHOQQ_RS23550"
                     /old_locus_tag="pgaptmp_004710"
     tRNA            complement(56..132)
                     /locus_tag="ACHOQQ_RS23550"
                     /old_locus_tag="pgaptmp_004710"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Asp,seq:gtc)
     gene            complement(265..996)
                     /gene="dnaQ"
                     /locus_tag="ACHOQQ_RS23555"
                     /old_locus_tag="pgaptmp_004711"
     CDS             complement(265..996)
                     /gene="dnaQ"
                     /locus_tag="ACHOQQ_RS23555"
                     /old_locus_tag="pgaptmp_004711"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGU4472851.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1061..1528
                     /gene="rnhA"
                     /locus_tag="ACHOQQ_RS23560"
                     /old_locus_tag="pgaptmp_004712"
     CDS             1061..1528
                     /gene="rnhA"
                     /locus_tag="ACHOQQ_RS23560"
                     /old_locus_tag="pgaptmp_004712"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGU4472852.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1525..2247)
                     /locus_tag="ACHOQQ_RS23565"
                     /old_locus_tag="pgaptmp_004713"
     CDS             complement(1525..2247)
                     /locus_tag="ACHOQQ_RS23565"
                     /old_locus_tag="pgaptmp_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4472853.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2281..>2824
                     /gene="gloB"
                     /locus_tag="ACHOQQ_RS23570"
                     /old_locus_tag="pgaptmp_004714"
     CDS             2281..>2824
                     /gene="gloB"
                     /locus_tag="ACHOQQ_RS23570"
                     /old_locus_tag="pgaptmp_004714"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU4472854.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPH"
BASE COUNT          738 a          698 c          678 g          710 t
ORIGIN      
        1 aaccgcaaaa ttaaaaatct tgcggttctc tgaactcatt ttcatgagtg aatagtggcg
       61 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
      121 aactaccgct ccgcgttgtg ttccgttggg aacgaggcga atattacgga ttgcctcacc
      181 cttcgtcaac ggtttttctc atcttttgaa tcatttgctg caaaaatcgc ccaagtcgct
      241 atttttagcg ccttttacat gtatttatgc ccgccagagg cagcttccgc cctttttctg
      301 caccagatcg agacgggctt catgtgctgc aagctcttca tctgtcgcaa aaacaacgcg
      361 taacttactt gcctgacgta caaggcgctg gattgttgct tcaccttgtt gctgttgtgt
      421 ctctccttcc atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc
      481 cgcaaggatc tgggcatcga gtaatgcccc gtgcagcgtt cgtttactgt tatctatttc
      541 gtagcgagca cataacgcat cgaggctgtt gcgcttaccg ggaaacattt tcctcgccac
      601 cgcaaggcta tcggtgacct tacagaaggt attggtcttc ggaatatcgc gcttaagcaa
      661 cgaaaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca ccaactccgc
      721 gccgcggata tagtccatga actcatcggc tacttcggca aacgtgggct tatcgagcaa
      781 aaattcatcg gcaataccat gtacaccaaa ggcttccgga tccaccagcc gatcgggttt
      841 gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa cggcaccaat
      901 ctcaatgatc ttgtggcctt catagtgcgc accaatctgg ttcataccgg tggtttcggt
      961 atcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg tcagacttgt
     1021 cgttttacag ttcgattcaa ttacaggaag tctaccagag atgcttaaac aggtagaaat
     1081 tttcaccgat ggttcgtgtc tgggtaatcc tggacctggg ggttacggcg ctattttacg
     1141 ctatcgcgga cgcgagaaaa cctttagcgc aggctacacc cgcaccacca acaaccgtat
     1201 ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt
     1261 gagtaccgac agccagtatg tccgccaggg tatcacccag tggatccata actggaaaaa
     1321 acgtggctgg aaaacagcag acaaaaaacc agttaaaaat gtcgatctct ggcaacgtct
     1381 tgatgctgcg ctggggcagc atcaaatcaa atgggaatgg gttaaaggcc atgccggaca
     1441 cccggaaaac gaacgctgtg atgaactagc tcgtgccgcg gcgatgaatc ccacactgga
     1501 agatacgggc taccaagtcg aagtttaagc ctgtggttta cgacattgcc gggtggctcc
     1561 aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcggattta gcgttaaagg
     1621 aatagtccgt ttccgggcaa caataagttg taagcaacca agcgcaggaa tatgcgcatt
     1681 caaaagtttt cctccgtgtt tgttccacgg gagaacgtgg aaacggctgg cgtgtagcac
     1741 ttcaaaattc aacaaagaga gccagtccag ctgccgcatc agagtaaaca tccggctgtt
     1801 atagggcgag gttttgcgca ataccggcac aagtttgcgt aatcccatta aactaatggg
     1861 attgaagcca ctaatcacca gccagccatc atcaatcaat acccgatcgg cttcacgcaa
     1921 taaacgatgc ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa catcaacgga
     1981 tttatcggca aaaggaagat gaagtggatc cgcctgcacc tggacgggca ttccttgcgc
     2041 agaaacattc acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt
     2101 gccaatctta agcagatgaa aaccatacat tttagtgaac cacgggttga gctggcgctc
     2161 cagcgccttg cgataaagct ctccccaggg caaatcgccc cagcgatcag gagccacgac
     2221 agtttgaggg actcttgccg gtttcatcac aaccttccgt ttcacactga gaggtaatct
     2281 atgaatctta acagtattcc cgcctttgat gacaattaca tctgggtttt gaatgatgaa
     2341 gcaggtcgct gcctgattgt cgatcccgga gacgcagagc cagtattaaa cgccattgcc
     2401 gccaataact ggcaaccgga ggccatattt ctcacccacc atcatcacga tcacgttggc
     2461 ggcgtaaaag aactggtgga aaagtttccg caaattgtgg tgtatggtcc acaagagaca
     2521 caagataagg gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat
     2581 gaatttagtg taattgccac accgggtcac actttaggac atatctgtta cttcagtaaa
     2641 ccttatctat tttgcggcga cacgctgttt tctggtgggt gtggtcggtt gtttgaaggg
     2701 acagcatcac aaatgtatca atcacttaaa aagttaagtg cgttacctga cgatacattg
     2761 gtatgttgtg ctcatgaata tacgttatca aatatgaagt ttgctttgag tattcttccg
     2821 cacg
//
LOCUS       DAZKFU020000388         2803 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZKFU020000388 DAZKFU020000000
VERSION     DAZKFU020000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.390"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..676
                     /gene="comR"
                     /locus_tag="ACHOQQ_RS23575"
                     /old_locus_tag="pgaptmp_004715"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="ACHOQQ_RS23575"
                     /old_locus_tag="pgaptmp_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGU4472855.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHOQQ_RS23580"
                     /old_locus_tag="pgaptmp_004716"
     CDS             complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHOQQ_RS23580"
                     /old_locus_tag="pgaptmp_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4472856.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..>2803)
                     /gene="ndh"
                     /locus_tag="ACHOQQ_RS23585"
                     /old_locus_tag="pgaptmp_004717"
     CDS             complement(1504..>2803)
                     /gene="ndh"
                     /locus_tag="ACHOQQ_RS23585"
                     /old_locus_tag="pgaptmp_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGU4472857.1"
                     /translation="TPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHL
                     WKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKG
                     ELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYS
                     ANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERI
                     LPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKA
                     PDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQ
                     MATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIAR
                     FVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          669 a          772 c          693 g          669 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgcgcc gtactcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtcagc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggctgg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tcgccaatac gctgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactgatgca gattgcccga acgactttgc gtttatggcc
      661 ggaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggctaaat
      721 tgcagagtac aaccttgtta tactttgtta ttcaaaatca gttgcccgtt gctatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc accaaactga
     1021 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgtgg gaatggctgg aaatacttaa
     1381 ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attctgtata gcgagatgta
     1621 cacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccgacg gtggagaagt tcgacagcga taccagcgaa ccgtgatctt tatactgata
     1741 atttttcagc ggcttaccgt tcatctgcgc cagaatgttg ttcattgcgc aagttgccat
     1801 ctggtgtgca gcctgagcgc gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcaatcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttgg tttcaagacc accaatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccacacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
     2041 gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttcacc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg accgcgttgt gcagttcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag
     2341 attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
     2461 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gcggtttcca caggtggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatctt tttcaatggc gta
//
LOCUS       DAZKFU020000389         2749 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZKFU020000389 DAZKFU020000000
VERSION     DAZKFU020000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.391"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2513
                     /locus_tag="ACHOQQ_RS23590"
                     /old_locus_tag="pgaptmp_004718"
     CDS             <1..2513
                     /locus_tag="ACHOQQ_RS23590"
                     /old_locus_tag="pgaptmp_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HGU4472858.1"
                     /translation="VTSQTAGISVVTASINSSSQSRDVTFIADVRTAQIADLVVIKDG
                     SVADGAMANMLRARVTDAFGNALGGQTVSVTAGNSATVTPTVTTQSDGTVEFSVTSQT
                     AGVSAVTATINNHSLSQNVTFVPGDASQLTSTVETNKSNYTVGETITITVTLRDAFDN
                     LVTGAASQLAANSVLTVDGTDPSETGSWVESGGVYTTTRMATIAGTDQHANLQLQTWS
                     DGVTSDRYDIQSGSPAQATSTIATDKNAYTAGETITVAVTLKDAHGNLVEGGESLLSG
                     DNVTVEGAVRSGEWSETAGVYTATWSAQMAGDSHHATLTLPEWGNSKQSESYSIHSGA
                     PVQANSAIRTDKSAYIAGEPLTVTITLRDEFGNPALGLTSEVIESYIDSFAVGGATPD
                     SMQWVEQNNGEYTIVWTAWVAEENLVASLKLKTWATEIKSSLYGIQPGAAAQTQSTIV
                     TDKTKYIAGDSITVTVVLKDAQGNFITDGVAQLNEENVQVRNADSIQGNNWVYNGNGQ
                     YQRQYMAHFAEANLNAQLKMAGWSDANYSKNYTINRGEVSMLRSQLRIREVLVVAGAD
                     IPVSVLLSDEFGNPVNDGLDLLTDDAVYLQNVEKKHWSSWTFVGDGRYERTYMAYKEG
                     ENLNSYLHINGWYVGGQPSYTILPFVEVESLSVNGAKFRAADGFPKTGFDGAKFTLIL
                     THNMKNTDYNWTSGIQGIQVDSNGMVTLEFIINKEVTITGTPKSNKGNKVTYKFSLQK
                     WFIPQGIIQESWSEMNSYCIGNGYILPSSTDLVGSSTSGAVPRKVGSLWGEYGNLTSY
                     DGIFRAEHYWLDSGMIFYPGDGHLSIAPRSSPLCMKTF"
     gene            complement(2563..>2749)
                     /locus_tag="ACHOQQ_RS23595"
                     /old_locus_tag="pgaptmp_004719"
     CDS             complement(2563..>2749)
                     /locus_tag="ACHOQQ_RS23595"
                     /old_locus_tag="pgaptmp_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4472859.1"
                     /translation="NKINGVILPLIYSVYGKKEYEKRWQQLIKESDLWSNVLLHDPRL
                     LSVKNEFNTIGVMRSSA"
BASE COUNT          771 a          580 c          711 g          687 t
ORIGIN      
        1 gtgtcaccag ccagacggca ggaatcagtg tggtcaccgc cagcattaac agcagcagcc
       61 agagccggga tgtgacgttt atcgccgatg tcagaacggc gcagatcgcg gacctggtgg
      121 tcattaagga cggctcagtg gctgacggcg caatggcgaa tatgctgcgg gcgagggtca
      181 ccgatgcgtt cggcaatgcg cttggcggac agacggtcag cgtgacggca ggcaacagcg
      241 caacggtcac tccgacggtc accacgcagt cggacggtac ggtggagttc agtgtcacca
      301 gtcagacggc gggagttagc gcagtcacag caaccatcaa caatcatagc ctgagccaga
      361 acgtcacctt tgtaccgggt gacgcatcgc agctcacttc aaccgttgaa acaaataagt
      421 ctaactacac ggttggagag accatcacca tcacggtaac gctccgggat gcgttcgata
      481 atctggtaac cggtgcagct tcacagttag ccgctaatag tgtgctgacg gtggatggca
      541 ccgacccgtc agaaacggga agctgggttg aatcaggtgg cgtgtatacc acaacccgaa
      601 tggcgacgat tgccggtacc gatcagcacg ctaacctgca actgcagacg tggagtgatg
      661 gtgtaacgtc agatcgctat gacatacagt ccggttctcc ggcgcaagcc acatcaacta
      721 tcgccacgga taaaaacgca tacaccgctg gcgagactat aaccgtcgcc gtgacgctga
      781 aagatgcgca cggtaacctg gttgaagggg gcgagtcctt actgtctggt gataacgtaa
      841 cagtggaagg agccgtacgt tcaggagagt ggtctgaaac tgccggtgtt tatactgcta
      901 catggtcggc gcaaatggcg ggagactctc accacgcgac gctgacgttg cccgagtggg
      961 gcaacagtaa acaatcagag agttattcca tccacagtgg tgctccggtg caggcaaatt
     1021 ctgctatcag gacagataaa tcggcatata tcgccgggga gccattaact gtcacaataa
     1081 cgttgcggga tgagtttggt aaccctgctt tagggctgac atcagaagtc atcgagtctt
     1141 atatcgatag tttcgcggta ggtggcgcaa ctcccgattc tatgcagtgg gtcgagcaga
     1201 ataatggtga atataccatt gtctggacag catgggttgc cgaggaaaat ctggttgcca
     1261 gtctgaaatt aaaaacatgg gcaacggaga ttaagtcctc attatacgga atacaaccag
     1321 gtgcggcggc acaaactcaa tccacgattg tcacggacaa aacgaaatat atcgctgggg
     1381 atagcataac ggtgaccgtt gttcttaaag atgcccaggg taactttatt actgatggtg
     1441 tcgcgcagct taatgaggaa aacgtacaag tcaggaacgc tgattctatt caaggcaata
     1501 attgggttta caacggcaat gggcaatatc aaagacagta tatggcgcat tttgcggaag
     1561 cgaacttgaa tgcgcaactg aagatggctg gttggagcga tgccaattat tcaaaaaact
     1621 acactattaa cagaggtgaa gtaagtatgc ttcgttcaca gcttcgtata cgcgaagttt
     1681 tagtggtagc tggggcggat atacctgtta gtgttttatt atctgatgaa tttggtaatc
     1741 ctgtgaatga cggtctggat ttgctgacag atgatgcagt gtacttacaa aatgtggaaa
     1801 agaaacattg gtccagttgg acatttgtag gtgatggcag atatgagcgt acctatatgg
     1861 cttacaaaga aggagaaaac ttaaactctt atctacatat taatggttgg tatgtaggcg
     1921 ggcaaccgtc ttataccatc cttccttttg tggaagtcga gtcgcttagt gtcaatggtg
     1981 caaaattcag ggcggcagat ggttttccca aaacagggtt tgatggtgct aaatttacac
     2041 tgatattaac ccacaatatg aaaaacacag attataattg gaccagtggc atacagggta
     2101 ttcaggtcga tagtaatggt atggtgactt tagaattcat tatcaataaa gaggttacta
     2161 ttacaggaac gccgaaaagc aataaaggga ataaagttac atataaattc tcactgcaga
     2221 aatggtttat acctcaaggg ataattcagg aatcatggag tgagatgaat tcatattgta
     2281 ttggtaatgg ttatatacta ccgtctagta cggatttagt tgggagttca actagcggtg
     2341 ctgtcccgcg aaaagtaggt tcgttatggg gtgaatatgg taatctgact tcgtatgacg
     2401 gaatatttcg cgctgaacat tattggctcg acagcggtat gatattttat ccaggagatg
     2461 gtcatctctc gattgcgcca cgttcatccc cactgtgtat gaaaacgttc tgatattatt
     2521 tatgttattg ccctgcatca gcagggcaat ttatcttcgc tcttacgcag aactccgcat
     2581 cacaccaata gtattaaatt catttttcac actgagcaaa cgggggtcat gaagcaacac
     2641 atttgaccat aaatcactct ctttaattaa ttgttgccag cgcttttcat attctttttt
     2701 accataaaca ctgtaaatta acggcaaaat aacaccattg attttgttg
//
LOCUS       DAZKFU020000390         2742 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZKFU020000390 DAZKFU020000000
VERSION     DAZKFU020000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.392"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1665
                     /locus_tag="ACHOQQ_RS23600"
                     /old_locus_tag="pgaptmp_004720"
     CDS             <1..1665
                     /locus_tag="ACHOQQ_RS23600"
                     /old_locus_tag="pgaptmp_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4472860.1"
                     /translation="KSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAFSDFTPT
                     EYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1717..>2742
                     /locus_tag="ACHOQQ_RS23605"
                     /old_locus_tag="pgaptmp_004721"
     CDS             1717..>2742
                     /locus_tag="ACHOQQ_RS23605"
                     /old_locus_tag="pgaptmp_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4472861.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFTKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEVFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVS"
BASE COUNT          834 a          599 c          569 g          740 t
ORIGIN      
        1 aaaagtgtcg taagtacctc gatatctttg atactggcat ctggtatggc tgcatttgct
       61 gctcatgcgg cagatgatgt aaagctgaaa gcaaccaaaa caaacgttgc tttctcagac
      121 tttacgccga ctgaatacag taccaaagga aagccaaata ttatcgtact gaccatggat
      181 gatcttggtt atggacaact tccttttgat aagggatctt ttgacccaaa aacaatggaa
      241 aatcgtgaag ttgtcgatac ctacaaaata gggatagata aagccattga agctgcgcaa
      301 aaatcaacgc cgacgctcct ttcattaatg gatgaaggcg tacgttttac taacggctat
      361 gtggcgcacg gtgtttccgg cccctcccgc gccgcaataa tgaccggtcg agcgcccgcc
      421 cgctttggtg tctattccaa taccgatgct caggatggta ttccgctaac agaaactttc
      481 ttgcctgaat tattccaaaa tcatggttat tacactgcag cagtaggtaa atggcacttg
      541 tcaaaaatca gtaatgtgcc ggtaccggaa gacaaacaaa cacgtgacta tcatgacaac
      601 ttcaccacat tttctgcgga agaatggcaa cctcaaaacc gtggctttga ttactttatg
      661 ggattccacg ctgcaggaac ggcatattac aactcccctt cactgttcaa aaatcgtgaa
      721 cgcgtccccg caaaaggtta tatcagcgat cagttaaccg atgaggcaat tggcgttgtt
      781 gatcgtgcca aaacacttga ccagcctttt atgctttacc tggcttataa tgctccgcac
      841 ctgccaaatg ataatcctgc accggagcaa tatcagaagc aatttaatac cggtagtcaa
      901 acggcagata actactacgc ttccgtttat tctgttgatc agggtgtaaa acgcattctc
      961 gaacaactga agaaaaacgg acagtatgac aatactatta ttctctttac ctccgataat
     1021 ggtgcggtta tcgatggtcc tctgccgctg aacggggcgc aaaaaggcta taagagtcag
     1081 acctatcctg gcggtactca caccccaatg tttatgtggt ggaaaggaaa acttcaaccc
     1141 ggtaattatg acaagctgat ttccgcaatg gatttctacc cgacagctct tgatgcagcc
     1201 gatatcagca ttccaaaaga ccttaagctg gatggcgttt ccttgctgcc ctggttgcaa
     1261 gataagaaac aaggcgagcc acataaaaat ctgacctgga taacctctta ttctcactgg
     1321 tttgatgagg aaaatattcc attctgggat aactaccaca aatttgtccg tcatcagtca
     1381 gacgattacc cgcataaccc caacactgag gacttaagcc aattctctta tacggtgaga
     1441 aataacgatt attcgcttgt ctatacagta gaaaacaatc agttaggtct ctacaaactg
     1501 acggatctac agcaaaaaga taaccttgcc gccgccaatc cgcaggtcgt taaagagatg
     1561 caaggcgtgg taagagagtt tatcgacagc agccagccac cgcttagcga agtaaatcag
     1621 gagaagttta acaatatcaa gaaagcacta agcgaagcga aataactaaa ccttcatgcg
     1681 gcggaatttt ccgccgcctt attgagcgag atagcgatgc acgttacagc caagccctcc
     1741 agttttcaat gtaatctcaa atgtgattac tgtttttacc ttgaaaaaga gtcgcagttt
     1801 actcatgaaa aatggatgga tgacagcact ctgaaagagt tcatcaaaca atatatcgca
     1861 gcgtctggca atcaggtcta ttttacctgg caaggcggtg aacccactct ggctggcctg
     1921 gattttttcc gtaaagttat tcactatcaa caacgctatg caggccaaaa acgtattttt
     1981 aatgcattac aaacgaatgg cattttattg aataatgaat ggtgtgcctt tctcaaagaa
     2041 catgaatttc tggttggtat ctcgatcgat ggcccccagg agttacatga ccgttacaga
     2101 cgcagtaatt caggtaacgg tacttttaca aaagtgatag cagccatcga gcgtctgaaa
     2161 tcatatcaag tagagtttaa tacgttaacc gtcattaata acgttaatgt ccattaccct
     2221 cttgaggttt atcatttttt aaaatctatc ggcagtaaac atatgcaatt tatcgaattg
     2281 ctagaaaccg ggacgccgaa tattgatttc agtggtcata gtgagaacac attccgtatc
     2341 attgattttt ctgtgcctcc cacggcttat ggcaagttta tgtcaaccat ttttatgcaa
     2401 tgggttaaaa acgatgtggg tgaagttttc atccgtcagt ttgaaagctt tgtcagccgt
     2461 tttttgggga atgggcatac cagttgtatt ttccaggagt cctgcaagga taatctggtt
     2521 gttgaaagta atggagacat ttacgaatgc gaccattttg tctatccaca gtacaaaatt
     2581 ggaaacatta ataaatctga actcaaaacg atgaacagtg tacaactgac agcgcaaaaa
     2641 aaacggattt cagcgaaatg tctgaaatgt gcatataaac ctatctgcaa tggcggttgt
     2701 cctaagcatc gtattactaa agtaaacaat gagactgttt ct
//
LOCUS       DAZKFU020000391         2741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZKFU020000391 DAZKFU020000000
VERSION     DAZKFU020000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.393"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(831..1829)
                     /locus_tag="ACHOQQ_RS23610"
                     /old_locus_tag="pgaptmp_004722"
     CDS             complement(831..1829)
                     /locus_tag="ACHOQQ_RS23610"
                     /old_locus_tag="pgaptmp_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HGU4472862.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFKRLKERKEFRHVLGWVRTTEVT
                     RGKDGTAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADDMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRQLSGED"
     gene            2504..>2741
                     /locus_tag="ACHOQQ_RS23615"
                     /old_locus_tag="pgaptmp_004723"
     CDS             2504..>2741
                     /locus_tag="ACHOQQ_RS23615"
                     /old_locus_tag="pgaptmp_004723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472863.1"
                     /translation="MLPERWRNIGSFCAVSPSTEARRSGNRTANHFAVFVPQPFRAID
                     KLAVGGESHEVRTYTISTSFRLCAVCDAVKTADNR"
BASE COUNT          686 a          788 c          641 g          626 t
ORIGIN      
        1 aaaaaaaaat ccccctgtgt cagggggccg tcaaagtcaa tttcctgatg atagttgttt
       61 tgaggtgttg ctatgcccgc tcaatttgcc gcaggaacac cgcaaaaaca actccgatga
      121 cgggacagga tgtgtaatcg tataaccgcg cacgcacgcc gatacgatta ccacctggtc
      181 agggctacgc cccgacaccc tcgacaacct gcccgggaca cgttaaaacg acatctgcgc
      241 gtttctgaag ctcccattaa ccatggggat ggtattagcg ccgggatgcg acagaaacgc
      301 gcttaaaacg cccctgaacg catcaaacgc gaaaaaacga acaaaacctt gcctgcctgc
      361 aaaaatcacg ttagaacgcg tctgagcgcg tctgaggggt taaaattcac tgtgcgtcaa
      421 atggggattg ttacaccccg ccctcaaatg cgctcacttc cccttcctgt ccatgtgcgg
      481 aaatgttgtt gctttgtatc ttttgtgtta agcaacggat gagcgtgttt ctttcgcatt
      541 tccactatca tcaacccacg aagacctgta gaaacacgct ccccccctcc gactactgga
      601 cagcagaatc acaccacgct aaacatgaca tgcgtgtttc tggtgagcga aaagctgtca
      661 gccgtggact gccgtagcag agacccggaa acgcgcttaa aacgcttctg agtgcgtcaa
      721 aacaaaaaac gagtaaatcc ttgctcgttt ctgaaaatca cgttagaacg cttctgagcg
      781 cgtttctgaa tattctccca gtgcatgaat agttcgcgcc tgaaacgcgc tcaatcctcg
      841 ccacttagct gacgtttatc tttcgcccga tcgcgctggt attttttgac aaccggcacc
      901 cacgaaaacg ccagacggga accgtcatct gtaccctctg ccatgtcatc cgccatgatt
      961 aaatcctcgt tcgtttcccg ctccagttgc agaacgttct taagcgctcc ccccgtagca
     1021 acaaaacgac gcttgtgtac ctgccgtgtt aattcgagga accattcagg atcagccagc
     1081 atatcagcag gctttgtgga atatttcaga gtttcaacca ccgcgccgcg cagctgttcc
     1141 gcaacaacac aaggtaaaga ttcatcgtct tttggtttcc gtgatttcac cactctgata
     1201 tccacattcg ggtcatacgc cacccgcaga caatcacgcc acagttccac ccaacgggca
     1261 tgtttcacat actcacggcc ggacaacatc gaagggggaa ccatcagcag agtgtgaaaa
     1321 tggggatgtg cagtaccatc cttgccgcgc gttacttccg tggtacgaac ccaacccaac
     1381 acatggcgaa attccttacg ctctttcaac cgcttaaacc ccgcattcat cgcggtcagc
     1441 gtctcaccaa gcgcgtcaat ggaacagttg cgcactgtca gtgtgagaaa caaccagcgc
     1501 gcgccgggat gttccgtcac aatctttggc agggactgat agaaacgggc ttgccacatc
     1561 atggaacgcc tccactgaca gatcggacag tgccggacgc gacagaaccg tgcatcacgc
     1621 aaccgcaggc gcgtttctcc ggtctctgtg tttgttgacc acccaaaccg caaaatacca
     1681 ctacaagccc cgacacgttc gccgtagtgc tcaaatccgg cagcacgcag ataaataccg
     1741 cccacatcgt cggaaaagct tcggtgttca tcccacggtt tatctgctgg agagtaatcc
     1801 gtcagattct taagttcagg atttttcatg tctgccgtcc ccttgcgagc gacagacgaa
     1861 attgcgtgtt atcattcctg tatcacctgt accaattgtc gtcaaatcag ttcatgttct
     1921 aggtggtcgc gcccctggag tggtggaaga ctcgcagggg ctaatttata accactcccg
     1981 acaaaatact ctcaaaccta tttacacgca aatccgcgct caggccagat atggcaaggg
     2041 atagagctga ttcaaaaaat cgcatttatc ccttaagtat aaatacaagg aacgcgcacg
     2101 aaagcagcag caaaatcgcc tgttttttgt tcgatttttt gagggcaaaa acaacaacca
     2161 cgaaagcgtt ttcccgacaa aacacccaca ggcttttcca ccaattttgt gaacaacaaa
     2221 aaccgtcttg caggtggttg gcatcactcc gtgacaccaa ccacctcctc cagtgttttt
     2281 tccgtgccat cgtcaatcac aaaatcggaa cccacgccgg aggcgcggga ccccacgatt
     2341 ttctgtttga cgaattcggg aacggtgagc agtacgcgga attttttcac gtgccgcgaa
     2401 gcgttggcag ggtgtcgggg cgtagccctg accaggtggg gaatgtctga gtgcgcgtgc
     2461 gcggtccgac attcccacat cctgtcccga tttctgcgag tttgtgttac cggaacgctg
     2521 gcgcaatatc ggcagttttt gcgccgtgtc gccctccacg gaagcgcgca gatctggcaa
     2581 ccggacggca aatcattttg ccgttttcgt gccgcagccg ttccgcgcga tcgacaaact
     2641 ggcagtcggg ggggaatctc acgaagtgcg cacttataca atctcaactt cgtttcgatt
     2701 gtgtgcggtc tgcgacgcgg tgaaaaccgc agataacagg c
//
LOCUS       DAZKFU020000392         2712 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZKFU020000392 DAZKFU020000000
VERSION     DAZKFU020000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.394"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..357)
                     /gene="quuD"
                     /locus_tag="ACHOQQ_RS23620"
                     /old_locus_tag="pgaptmp_004724"
     CDS             complement(<1..357)
                     /gene="quuD"
                     /locus_tag="ACHOQQ_RS23620"
                     /old_locus_tag="pgaptmp_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HGU4472864.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEM"
     gene            complement(443..583)
                     /locus_tag="ACHOQQ_RS23625"
                     /old_locus_tag="pgaptmp_004725"
     CDS             complement(443..583)
                     /locus_tag="ACHOQQ_RS23625"
                     /old_locus_tag="pgaptmp_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU4472865.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGYVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(580..942)
                     /gene="rusA"
                     /locus_tag="ACHOQQ_RS23630"
                     /old_locus_tag="pgaptmp_004726"
     CDS             complement(580..942)
                     /gene="rusA"
                     /locus_tag="ACHOQQ_RS23630"
                     /old_locus_tag="pgaptmp_004726"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HGU4472866.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(939..1229)
                     /locus_tag="ACHOQQ_RS23635"
                     /old_locus_tag="pgaptmp_004727"
     CDS             complement(939..1229)
                     /locus_tag="ACHOQQ_RS23635"
                     /old_locus_tag="pgaptmp_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU4472867.1"
                     /translation="MVDLRKAARGRECQVRIPGICNGNPETSVLAHIRLAGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(1222..1392)
                     /locus_tag="ACHOQQ_RS23640"
                     /old_locus_tag="pgaptmp_004728"
     CDS             complement(1222..1392)
                     /locus_tag="ACHOQQ_RS23640"
                     /old_locus_tag="pgaptmp_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU4472868.1"
                     /translation="MTTPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(1392..1847)
                     /gene="ybcN"
                     /locus_tag="ACHOQQ_RS23645"
                     /old_locus_tag="pgaptmp_004729"
     CDS             complement(1392..1847)
                     /gene="ybcN"
                     /locus_tag="ACHOQQ_RS23645"
                     /old_locus_tag="pgaptmp_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HGU4472869.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(1844..1942)
                     /locus_tag="ACHOQQ_RS23650"
                     /old_locus_tag="pgaptmp_004730"
     CDS             complement(1844..1942)
                     /locus_tag="ACHOQQ_RS23650"
                     /old_locus_tag="pgaptmp_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472870.1"
                     /translation="MRPQVELLALPQMQKCTCVFLHPTFDLCGGEA"
     gene            complement(2018..2269)
                     /locus_tag="ACHOQQ_RS23655"
                     /old_locus_tag="pgaptmp_004731"
     CDS             complement(2018..2269)
                     /locus_tag="ACHOQQ_RS23655"
                     /old_locus_tag="pgaptmp_004731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472871.1"
                     /translation="MMSFTKCKLMHSHKTSVNAALLDDTKLVVERDLHFLRELYISSP
                     EEFVGHWVYANEPIFHEHPLSVGLSGIVYHKVIDEIEKS"
     gene            complement(2329..2658)
                     /locus_tag="ACHOQQ_RS23660"
                     /old_locus_tag="pgaptmp_004732"
     CDS             complement(2329..2658)
                     /locus_tag="ACHOQQ_RS23660"
                     /old_locus_tag="pgaptmp_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HGU4472872.1"
                     /translation="MDAGVLVLAVQQSPITKQFTDNELCTLAWLWRAGNVMLIAYQNV
                     THLLQDAEHGEAGHFTSIEQEYPRILNRARAILVRETAHVKLQPWQDDKWSRVLPHLR
                     QNVIKQR"
BASE COUNT          692 a          678 c          608 g          734 t
ORIGIN      
        1 catctctaac cggatatcta atgccattaa catcccttca attatgccct cagccttctg
       61 taaccttttc ccgatataac catcagagca gcaatgctta cctgccagtg acatgaatgt
      121 cataccgact acataataat ctactaataa atcgtgcaaa tcgctgttgt tctttttcag
      181 acgggccatg cacccgcaaa tgatcatcgc gtcatcgtca caacattgcg ggcgagattt
      241 tacttttgaa ggaattaatc ccttaaaacc ggcggcaatg gacgaccagg tcacatcttc
      301 atgattatta gccgcccacg ctccccaacg ctcaagaacc atctgaatat cacgcatcaa
      361 cttactccac aaaactcaga ccagaacgcc aattacaagc aaaaatcaac aaaacagtat
      421 tagttgattg ttatctctga cttcatactc ctgctcctgt cagtgttttg gcgtaattct
      481 tcagtattcg gtaatcggtc aaaacataac cggggaaacg atataagcgc agacgccccc
      541 agcggtggcg aagaagttct gccatattaa actcaaacat cattcattcc ccatttcggt
      601 gatggtcagt tccagcctcc cacctttggt aacaggcatc ttcacaacgc ggtaatcaac
      661 gacctgagca tcatccagcc agaaacctgc tttagtgagt gcgtcaaaag cggctttttg
      721 cagattatcc aggtcacggc gacggcgatc cggcatgtgg cactcaatgc ggattttcac
      781 aggcatagcc aggccgatat ccagcattgc gtttttaatg attcgggcga cgttatcgcg
      841 gtatgcctgc ccctctgcgc tgatgtgcgt gcgcccgcga ttatggcggt aatagcgatt
      901 attgctcgga ggccagggta atgtgatgct gtaggtattc acgccttaat aaccccctct
      961 ttcagccaga taacctgtgt tctcgccata ccttccagcg cgcattcttt tgcatatcca
     1021 gcgtcaacaa aatgcgtgcg acggtcgatc tcatcgtgac aggcagaaca tgcaatggtg
     1081 gcaatcaggt ctggcggttt ggtaccggtg ccacacaatc cagccagccg gatatgtgcc
     1141 agtacagacg tttcagggtt gccattacat atgccaggga ttcttacctg gcattcccga
     1201 ccacgcgctg cttttctcaa atcaaccatg attcctcctt gctgccagtc gcaaccattt
     1261 tttatcaacc aggctggcgg tataccctag cagtgttggt atttcggatg gcttcagctc
     1321 aggcttacgc ttacgacgat ttggtactct gtagatgtgt ccgttcatga cacgaataag
     1381 cggtgtagtc attacgcctc ctgcttgtcg cgcagcagct ggaactcgca gctctgtgga
     1441 atagtcaggt ggcaaccaat attcatcgcc caggcttcaa ccttacacag gaagacatac
     1501 atctctccgg tatcaagatc ggaggtatgg cgtaacgact ggatcgtagt gatttcgccg
     1561 gttacgacat caaccaggtc cttggtttca taaccgaggt atgtgtgttt gagagcatct
     1621 tttacccatg ctgcagtagc gaacgatttc cccctgctga tgaggtattc actgatttcg
     1681 ctgtaccaca tgtggctgag tgcattctgg gaaagactgc gtctttcgcg ccacggttta
     1741 agcaccatgc gaaagcattt gccgtcctcc agataaggct ggatctgctg accgatagcg
     1801 gtgaagttac cgcgatgcaa tttgataccg tcttgtggga ggttcacgct tcacctccgc
     1861 agaggtcaaa ggttggatgc aaaaaaacgc aggtgcattt ctgcatctgt ggcaaggcga
     1921 ggagttcaac ttgtggtcgc atataatttc ccaatcatat gcgcagaagt caccgcaggg
     1981 tgttcaggcc aacggtgacg gtattattgc ttattgatta tgatttttca atttcatcaa
     2041 taaccttgtg atagacaata ccagaaaggc caacagacag tggatgttca tgaaatattg
     2101 gctcatttgc ataaacccaa tgcccgacaa attcttctgg gcttgatatg tataactcac
     2161 gaagaaaatg gagatccctc tctacaacta atttcgtatc gtccaagagc gctgcattta
     2221 cgctagtttt atggctgtgc attaatttac atttggtgaa ggacatcatg gcaaaagctc
     2281 ctttttctta tgaatccata tgttagctaa attggagaga cacagtagct aacgttgttt
     2341 aataacattc tgacgcaaat gtggcaatac tcgactccac ttatcatcct gccacggctg
     2401 aagttttaca tgtgccgttt ctcggacgag gattgctcgc gctctgttga gtatccgggg
     2461 atattcttgc tcgatggaag tgaagtgacc agcttcacca tgctccgcat cctgaagaag
     2521 atgagtaacg ttctggtagg caattaacat cacattccct gctcgccata accaggcgag
     2581 tgtgcaaagt tcgttatcag tgaattgttt tgtgattggg gattgttgaa ctgctagaac
     2641 gagaacgcca gcatccattg gcagtcccta tagtaaaacc atagctcagg acgcttcgtt
     2701 caggatagat aa
//
LOCUS       DAZKFU020000393         2709 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZKFU020000393 DAZKFU020000000
VERSION     DAZKFU020000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.395"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..1478
                     /gene="glpT"
                     /locus_tag="ACHOQQ_RS23665"
                     /old_locus_tag="pgaptmp_004733"
     CDS             120..1478
                     /gene="glpT"
                     /locus_tag="ACHOQQ_RS23665"
                     /old_locus_tag="pgaptmp_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGU4472873.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            1483..2559
                     /gene="glpQ"
                     /locus_tag="ACHOQQ_RS23670"
                     /old_locus_tag="pgaptmp_004734"
     CDS             1483..2559
                     /gene="glpQ"
                     /locus_tag="ACHOQQ_RS23670"
                     /old_locus_tag="pgaptmp_004734"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4472874.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT          661 a          588 c          737 g          723 t
ORIGIN      
        1 caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag aaacggcagg
       61 ttctctcact gaatcaggct gttaatcata aataagacca cgggccacgg aggctatcaa
      121 tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgccgcg gagatcgatc
      181 cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc tatgctgctt
      241 actatttggt tcgtaagaac tttgcgcttg ctatgcctta tctggttgag cagggattct
      301 cacgcggtga tttaggtttt gccctttcgg ggatctcgat tgcttatgga ttttcgaaat
      361 tcatcatggg ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc gcaggtttga
      421 ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg tcgagcattg
      481 cggtgatgtt tgtactgttg ttcctctgcg gttggttcca ggggatgggg tggccgccgt
      541 gtggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt gtgtcagtgt
      601 ggaactgtgc gcacaacgtc ggtggtggta ttccgccgct gctgttcctg ctggggatgg
      661 cctggttcaa tgactggcac gcggcgctct atatgcccgc tttttgcgcc attctggtgg
      721 cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ctgtggcttg ccgccgatcg
      781 aagagtacaa aaatgattat ccggacgact ataacgaaaa agcggaacag gagctgaccg
      841 cgaagcaaat cttcatgcag tacgtgctgc cgaacaaact gctgtggtac atcgccatcg
      901 ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg acttatctga
      961 aagaggttaa gcatttcgcg ctggataaat cctcctgggc ctacttcttc tatgagtatg
     1021 cgggtattcc gggcaccctg ctgtgcggct ggatgtcgga taaagtcttc cgtggcaacc
     1081 gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc gtttactgga
     1141 tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc ggcttcctga
     1201 tctacggccc tgtgatgctg atcggtctgc atgcgctgga actggcaccg aaaaaagcgg
     1261 caggaacggc agcgggcttt accggtctgt ttggttacct gggcggttcg gtggcggcga
     1321 gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt atggtaatga
     1381 ttggcggcag cattctggcg gttatcttgt tgattgttgt gatgattggc gaaaaacgtc
     1441 gccatcagga attactgcaa aaacgcaacg gaggctaatg gcatgaaatt gacgctgaaa
     1501 aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc aatggcggcg
     1561 gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt gccggagcat
     1621 acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga acaggatttg
     1681 gtgatgacca aagacgacca tctggttgtt ctgcatgacc attacctcga tcgtgttact
     1741 gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta cgcgatagat
     1801 ttcacgctgg atgaaattaa gtcgttgaaa tttaccgaag gtttcgatat tgaaaacggt
     1861 aaaaaagtgc agacttatcc agggcgtttc ccaatgggta agtccgactt ccgggtacac
     1921 acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg gaaaaatatc
     1981 ggtatctatc cagaaatcaa agcgccgtgg ttccatcatc aggaagggaa ggatattgcg
     2041 gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaagacga taaagtttat
     2101 ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga acccaaaatg
     2161 ggcatggatc tcaatctggt acagctgatt gcctataccg actggaatga aacgcagcag
     2221 aaacagccgg acggaagctg ggttaattac aactacgact ggatgtttaa gccgggtgcc
     2281 atgaaacagg tggcggaata tgcagatggt attggtccgg attaccatat gttgattgag
     2341 gagacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc tcagcagaac
     2401 aagctggtag tgcatcctta taccgtgcgg tcagataaac tgcctgaata cacaactgat
     2461 gtgaatcagt tatatgatgc tctgtataac aaagcgggtg taaatgggtt gtttactgat
     2521 ttccctgata aagcggttaa attccttaat aaagagtaat gctgctactg caggatggcg
     2581 ttcatgcgcc atcctctgct aaagcgaaat aatgctacta ccggttattc ttttcattaa
     2641 ccggtgcatc ctgtaaaaaa gatgccaaca taattttcgt aaaactcatt cgttaaaaaa
     2701 gctaacact
//
LOCUS       DAZKFU020000394         2700 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZKFU020000394 DAZKFU020000000
VERSION     DAZKFU020000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.396"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..317)
                     /locus_tag="ACHOQQ_RS23675"
                     /old_locus_tag="pgaptmp_004735"
     CDS             complement(<1..317)
                     /locus_tag="ACHOQQ_RS23675"
                     /old_locus_tag="pgaptmp_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU4472875.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSG"
     gene            complement(470..922)
                     /locus_tag="ACHOQQ_RS23680"
                     /old_locus_tag="pgaptmp_004736"
     CDS             complement(470..922)
                     /locus_tag="ACHOQQ_RS23680"
                     /old_locus_tag="pgaptmp_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HGU4472876.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            complement(948..2498)
                     /locus_tag="ACHOQQ_RS23685"
                     /old_locus_tag="pgaptmp_004737"
     CDS             complement(948..2498)
                     /locus_tag="ACHOQQ_RS23685"
                     /old_locus_tag="pgaptmp_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HGU4472877.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVHFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
BASE COUNT          792 a          638 c          478 g          792 t
ORIGIN      
        1 ataccactac gtgtactttt actgatgtca atcagataaa caggatcccc tttttttatc
       61 aactgtcctt catgaacaaa ctgcctgaca acaaatccct gtacacctga atatatattg
      121 acagctcttg gccaggttgt gacctcacca ctgacattaa cacggcggct ataggtacca
      181 acaataatga acatcagaaa tgccgtaata aacactatgc ttcccagcat gattaaccat
      241 agtggtattc cgggaagtaa tattgcccgt ccctgccact tcatttttct gttttctaaa
      301 gcatcctgac gaaacaaaat agctcctctt actcacactt ttcctgtaat accttcactg
      361 gttaagcgaa atgctataat caaagccaga aaatacagag tcctgtcaaa atagtatcag
      421 cgatgcattt accccgtaat ttacaattat ctctgtatct tcagaggtac tacgttttat
      481 gcagccgctc agggatgtat gtcacaattt tctttcgcct gcttaaccat cgtacctctc
      541 cttctcccca tggctgaagc tgtatgagat agtcaataac ttttcgacca aagcctggtt
      601 tacaacagaa atccagaatc caaatccttc catcttcatt ccattcagac ggatgcaacc
      661 tgaattctgg atccctgagc aggcgcgctt ctgtatcagc ctcaagatat gcccaggtaa
      721 tatacgccag tggaaaacct cgtgaatcaa acaggaattt aatctgatta tgctgtatcg
      781 catattcagt ccagtattga atggttaaca ccggatacag actgtaatta cgattgtaga
      841 gcatacaact gaccgcaaaa ccgatctgtt cataaacagt ccattcatct tttcctctga
      901 tggtttccct tatataagac atgattacta tttcccttat atatttttta cactgtgttt
      961 cttatcggtt caagatatcc aaatgcatct ctggaaaact taagcataaa atttttcata
     1021 tcagaaattg cttcttgtaa aaaaagggga tcatcgccat gattatccag caggatggca
     1081 attttttcgc acatattttc tttcctcagc tcttccctgg gttgcagcct tttcatggta
     1141 tgatgttcaa tttctttcag gagtaatgag gcatttatct gacgtctggt actgccttgc
     1201 gtcagccagt ggatggcctg ggaaaaacac tgttgtgcaa cggtgaaatg aacatcattc
     1261 ttgatgctac tattcagtgc ctgcttttga ttatgcctat gatggattag cgtcacaacc
     1321 aacttttgca atggtgaacc atcctgattg acaatatctt gggcatgtga tttctcgcag
     1381 tattgattga gtaatgcaac aaaactgtta accagttctt tcaatatatc aggtatctct
     1441 ttttgtgtta taaattctcc aggaataccc aacagttcta gataacagta ataacatgct
     1501 tcatgaaaag ttttctgaaa tatctcctca accttcaaac gggctgcaag agtgaagtca
     1561 gggtcaaaca tatattttcc gatatacaag cagagatcag acagcccttg cataagtacc
     1621 atcggttctt ttgccagttc agctaatctt agtttatacc caaaatcagc tggggaatct
     1681 cccggaaaca gacaactgag tccgttaaaa tccattaatc tgccatccat tgcaatatta
     1741 gaaggagaag ttgtcaatct caggaaacgt gttcgacaaa atgccatttg ccaggcctca
     1801 cgacgtgcca gttcacataa cccctcaatg caataaatcc ggggatcagt tcgtgcttca
     1861 gcatcaattt cttccggtgg tacaggtaga tatcctggca gcttgtggat cacagatcta
     1921 acccgacagg catcgtgaat taacagactg gaatactccg gttttacttg gaagtaaggt
     1981 gcccgttcaa aatgcgccgg acgaacaaca ggctcacgta ccaacagggc tcttcgtgat
     2041 ttcctaccgg agcgctcaaa tgccttttca gtatagagat ctgtaagtaa aaccgcccga
     2101 acccgcacag cactataagg taatatttga gccagtactt ctccccacaa agcctcatat
     2161 attgcatgaa cagcgccgag tgcaccatta gaatgacgtt cgtcagtacc ttcgccaacc
     2221 aacggattac ttcctattcc tttaacctgg tagttaccat caaacccaca acgtgcacca
     2281 cctccattac gctgaatacc actgccacca tagcgctctg catggaacac tttggcagaa
     2341 gctgtactgc caggatcgat aatataactg caggcatttt gcagctcgtc tggatttctg
     2401 acatcctctg cacaccagat taatgaggcc ttgatctgcc tggcagtgaa aggcaccagg
     2461 ttttcattca gttcagaaag tgaacactga tgactcatct ggcagtattc tccgtttcaa
     2521 ttttcataaa agagtgttca taaaagagca agtaagtgtc ctgacagctt cttccttatt
     2581 acagtaaaac tctattaatt acacataaat taaaaaatta tttatcaacg actgtaaatc
     2641 atatcttcat cagtaaagtg ttgaacataa aaacacactt ataaatggat cttctatctg
//
LOCUS       DAZKFU020000395         2698 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZKFU020000395 DAZKFU020000000
VERSION     DAZKFU020000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.397"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..1342
                     /gene="dcuC"
                     /locus_tag="ACHOQQ_RS23690"
                     /old_locus_tag="pgaptmp_004738"
     CDS             71..1342
                     /gene="dcuC"
                     /locus_tag="ACHOQQ_RS23690"
                     /old_locus_tag="pgaptmp_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4472878.1"
                     /translation="MFNVITGCVILIITVVLLIKRYDTRTTLIGAGILLCIVAMSPLN
                     AFSAFASRMTESGLIQAICTSMGFACVMKFTGCDTCLVHFLTKGLSRLGIFLIPAAVT
                     VSFIVCIAIPSAAGASAAVGATLIPLLISARIHPAIAGAAILSGTIGAYLNPGVAHNS
                     FISKISQTPLMEFVVFHTPTTITCGVICAVILTVVAIVRKETKCDSAGNISEQTQVEF
                     TDRDICFYLKSVAPFLPVALLMLAEFGFLGETKITVPAAMILGCLYALIVSKSNPKEL
                     VSSFFEGMGTSYANILGIIISAAVFIEGLRSVGIIDVFIRFLTDSPEFARWGGTLGPF
                     LMGLITGTGDATAFAFNEVVTPHANNFGYDTNTLGAAVMLASALGRNMSPLAGATIIC
                     AGLAGVNPVEIAKRTAPGMIVTVLFIALLFL"
     gene            1355..2665
                     /locus_tag="ACHOQQ_RS23695"
                     /old_locus_tag="pgaptmp_004739"
     CDS             1355..2665
                     /locus_tag="ACHOQQ_RS23695"
                     /old_locus_tag="pgaptmp_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HGU4472879.1"
                     /translation="MSELNQLQKWRREFHHIPESGWCEFITTSKIITILRGMGWQVIT
                     GNKYLSTEYIMGRNATEVADSIKRAVDSGVPHELLDEMDGFTGCVALWDTGKNGAVTA
                     FRFDIDCVQVSESKSKDHIPEKMGFASIHSGLMHACGHDGHISIGLGLAQWVSENNHK
                     LCGKIKLIFQAAEEGVRGAKPIAEGGILDDCDYFLSSHIGMGVPTGNVVASPTDLLCT
                     TKMDFFFYGTPAHAGVNPHLGNNALAAACHCATQLLAIPRHGEGMSRINIGTLNAGEG
                     RNIIPSFARMQLEVRGETETINQYMREHALKIAQGISSIFGLRFEYKVVGEATDLHND
                     PLLVNLIHYIAQEDMNMSLTEKSFGGSEDATLLIRRVQKIGGKAAYIILGSDLKAGHH
                     EVDFDFDENVLDTGVKLFSLCLERLNSPGLNKIVGNGKLESELY"
BASE COUNT          752 a          499 c          609 g          838 t
ORIGIN      
        1 ttgttcatga tcaatatttg agcaaatcac ataaagtaaa gattatcctt ctttgaagaa
       61 ggaggatctc atgtttaacg tgataactgg atgtgtgatt ctgataatca cagttgtgct
      121 tttgataaaa agatacgaca cacgaacgac actaattggt gcaggtattc tgctatgtat
      181 agttgccatg tcacctttaa acgcattttc ggcatttgca agcagaatga ctgagtccgg
      241 tctcattcag gccatttgta ccagtatggg ctttgcctgt gttatgaaat tcactggttg
      301 tgatacatgc ctggttcatt ttctaacgaa aggattatct cgtcttggta tctttcttat
      361 tcctgctgcg gttaccgtca gttttattgt ttgcatcgcg attccatctg ctgctggagc
      421 atcagctgct gttggtgcaa cgctgatacc attacttatt tcagcacgta ttcatcctgc
      481 aatagccgga gcagctatcc tttcaggaac tatcggtgct tacctcaatc ccggtgttgc
      541 acataattca tttatatcta aaatatcaca aacaccatta atggaattcg tcgtattcca
      601 tactccgact acaataacat gtggtgtaat atgtgctgtt attcttacag ttgttgctat
      661 tgtaagaaaa gaaacaaaat gtgattctgc aggaaacata tcagaacaaa cacaggttga
      721 gttcactgac cgtgatatct gtttttatct gaaatctgta gcaccatttc ttcccgtagc
      781 attacttatg ctggctgaat ttggttttct tggagagact aaaataaccg ttccagccgc
      841 aatgattctc ggttgtcttt atgccctgat tgtcagtaaa tccaatccca aagaattagt
      901 ttccagcttc tttgagggta tggggacatc atatgcaaat attcttggga tcataatttc
      961 cgcagctgtt ttcattgaag gtttaagatc tgttggtatc attgatgtgt ttatcagatt
     1021 tcttactgac tcaccagaat ttgcccgctg gggcggtaca ttggggccat tcctgatggg
     1081 acttataacc ggcacagggg atgcgactgc attcgcattc aacgaagttg tcacaccaca
     1141 tgcgaacaat tttggttacg atacaaatac tctgggtgct gcagttatgc tggcttctgc
     1201 gctagggaga aacatgtctc cgttggctgg tgcaactatt atctgtgccg gtctggctgg
     1261 agttaatcca gttgaaattg caaaacgcac tgcccccgga atgattgtga cggtattgtt
     1321 tattgcatta ttattccttt aacaggaggc gtatatgtct gaactgaatc agttacaaaa
     1381 atggcggaga gaatttcacc atattccgga aagtgggtgg tgcgaattta tcacaacgtc
     1441 aaagataatt actattctgc gtggtatggg atggcaggtt attacgggga ataaatacct
     1501 gtcaacagag tatattatgg gccgtaatgc aactgaagtt gcagatagta taaaacgtgc
     1561 agttgattca ggcgttcccc atgaattgct ggatgaaatg gatggcttta cagggtgtgt
     1621 tgctttatgg gatacaggaa aaaatggagc tgtcactgcg tttcgttttg acattgattg
     1681 tgtacaggta agtgaatcaa aatcgaaaga tcatattcct gaaaaaatgg gatttgcatc
     1741 catacactct ggcttaatgc atgcatgtgg acatgatggt catatatcga ttggtttagg
     1801 attggcacaa tgggtatctg aaaataacca taagttatgc gggaaaatta agctaatttt
     1861 tcaggctgca gaagaaggcg ttcgcggtgc aaaacctatt gcagaaggtg gtattcttga
     1921 tgactgcgac tacttccttt cttcacatat tggtatggga gttcctacag gaaatgttgt
     1981 cgcttcacct acagatttgt tgtgtacgac aaaaatggat ttctttttct atggtactcc
     2041 ggcacatgct ggtgtaaatc ctcacctggg aaataatgct ctggcagctg cttgtcactg
     2101 tgcaacgcaa ttacttgcca ttccacgcca tggtgaaggt atgtcccgga taaatattgg
     2161 cacgctaaac gcaggtgaag gaaggaacat aatcccgtct tttgcaagga tgcaacttga
     2221 agttcgtgga gaaacggaaa caataaatca atacatgcgt gaacatgctc tgaaaatagc
     2281 acaaggtatt tcttccatat ttggtttacg ttttgagtac aaagtggttg gtgaagcaac
     2341 ggatttacat aatgaccctt tacttgtcaa tctgatacac tatatagcac aagaagatat
     2401 gaacatgtct ttgacagaga aatcatttgg gggcagtgaa gatgcaacgc tccttattcg
     2461 ccgtgtacag aagataggag gaaaagcagc atatataata cttggcagtg atttaaaagc
     2521 tggtcatcac gaagttgatt ttgattttga tgagaatgtg ctggatacag gcgttaaact
     2581 gttttcgtta tgtctggaga gattgaactc tcccggacta aataaaattg tagggaacgg
     2641 aaaactggaa tctgaattgt attgattgct gacaggtatc ctgtcaatca ctcctgat
//
LOCUS       DAZKFU020000396         2673 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZKFU020000396 DAZKFU020000000
VERSION     DAZKFU020000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.398"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..589
                     /gene="eco"
                     /locus_tag="ACHOQQ_RS23700"
                     /old_locus_tag="pgaptmp_004740"
     CDS             101..589
                     /gene="eco"
                     /locus_tag="ACHOQQ_RS23700"
                     /old_locus_tag="pgaptmp_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGU4472880.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(965..2611)
                     /gene="mqo"
                     /locus_tag="ACHOQQ_RS23705"
                     /old_locus_tag="pgaptmp_004741"
     CDS             complement(965..2611)
                     /gene="mqo"
                     /locus_tag="ACHOQQ_RS23705"
                     /old_locus_tag="pgaptmp_004741"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGU4472881.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          622 a          746 c          676 g          629 t
ORIGIN      
        1 ctttaagtta ttcataaagc aaattaataa atctgatgaa tatgttaacc ttcagcgaca
       61 tcatcggtgc aaacctataa atgaagaagg aaagcaaaaa atgaagacca ttctacctgc
      121 agtattgttt gccgctttcg ccaccacttc cgcctgggcg gcagagagcg tccagccact
      181 ggaaaaaatc gcgccttatc cacaagctga aaaaggcatg aagcgtcagg tgattcagtt
      241 aaccccgcaa gaagatgaat ctaccctgaa agtagaactg ttaatcggtc agacgctgga
      301 agtcgattgc aatttgcatc gtctcggcgg gaagctggaa agcaaaacgc tggaaggctg
      361 gggctatgat tattatgtct ttgataaagt tagctcgccg gtttccacga tgatggcctg
      421 cccggatggc aagaaagaga agaaatttgt caccgcgtat ctgggcgatg ctggaatgct
      481 gcgttacaac agcaagctgc cgatcgtggt gtatacgcca gacaatgtag atgtgaagta
      541 ccgcgtctgg aaggcggaag agaaaattga caacgcggta gttcgctaaa ctgccgtgaa
      601 gtgcggcacc ccataggttg gataaggcgg ttacgccgca tccaacatct aacgcccgag
      661 ccggttgcct gatgcgacgc tgacgcgtgt tatcaggcct acaccgctgt gaagtgctcc
      721 accccgtagg tcggataagg cgggaacgcc gcatccgaca tccaacgtcc gagccggttg
      781 cctgatgcga cgctgacgcg tcttatcagg cctacaccgc tgtgaagtgc tccaccccgt
      841 aggtcggata aggcggttac gccgcatcca acatctaacg cccgagccgg ttgcctgatg
      901 cgacgctggc gcgtcttatc agcatacgcc acatccggca taccatgccg gatgtggcac
      961 atcattacaa cgcaatatcc gccacttctt tttgaacggg ttgcggtttc aactgcggtt
     1021 tcggcgtact atctgctgct tgcggtttgt cgtatttcag ccccagcact tcgctggtgt
     1081 actgcaactc gcgctctgtt gcggctacat caccgttcag cttgcgtcca taagacggaa
     1141 cgatcgcttt caacgtagcc tgccattgcg ggctggaaac gcgatcgcca aatacttttt
     1201 ccagcagatc caacataatc ggcgcggcgg ttgacgcccc tggcgatgcc cccaggagcg
     1261 cggcaatggt cccttgctgg tcactgacca cttcagtacc cagacgcagt acaccacctt
     1321 tatcggcatc acgcttgata atctgcacac gctgccccgc ttgccacaaa cgccagtcct
     1381 cttttttcgc ttgcggatag tactctttca acgcttcaaa acgatcctct tcactcaaca
     1441 tcacctgact caccagatat ttcaccagat cgaaattatc cagcccgacg tgcatcatcg
     1501 gcatcacgtt agaggtggtg gtggaactca ttagatccca caatgaaccg tttttgagga
     1561 atttggttga gaaggtggca aatggcccaa acagcactac gcgtttaccg tccagaacgc
     1621 gggtatcgat atgcggaacc gacatcggtg gtgcgccaac ggatgcttta ccgtaaacct
     1681 tcgccagatg gtgattaacc acgtccgggt tttccgaaac aaggaactga ccgcccaccg
     1741 ggaaaccggc gtagtctttc gcttccggaa tccccgattc ctgtaacagc ttcagtgccg
     1801 caccgcccgc gccgataaag acaaatttgg cacggatgtt ctgtgcagtg ccatttttca
     1861 gatcggcaac ggtaacggtc caggtgttat cgtcattacg ctttagggcg cggacttcgc
     1921 tgctgagttg cagcgagaag ttagatttct tctgcaagga agcaattaac tggcgggtga
     1981 tctcgccgta gttcacatcg gtaccaattt ccgtacgcgt ggctgccact ttctgttgcg
     2041 gatcgcgccc ttccatcact aacggtgccc actctttgat ctgcgcgtga tcttcagagt
     2101 aacgcatacc gcgaaatagc gagctttgtt gcaacgcggc gtaacgggcg cgcaggaaat
     2161 tgacgttatc ctcgccccag acaaagctca tatgcggaac ggtattgata aatgaacgcg
     2221 gagtacgcag cacgccgcgc tcgacctggt gcgcccagaa ctggcgggaa atctgaaatg
     2281 cttcgttaat ggcgactgct ttttcaatac tgatgctgcc atcggcgttt tgcggggtgt
     2341 agttcagttc catcagtgca gaatgcccgg ttccggcgtt gttccagccg ttcgaactct
     2401 cctgcgcgac cccctccagg cgctccacca tggtcatcga ccattcaggc tccagctcgc
     2461 gtaaataggt ccccaacgtg gcgctcataa tgccgccgcc aatcaacagt acatcagttt
     2521 cctgctcctc ggacgctttc gcttttgccg ccatcgaaac ggcattaagc cccacggcca
     2581 tcgagaagag catggcagtc acttttttca tcttgttaat gccttacttt tagtcgcttt
     2641 attgcaggtg agatttgcgc ggcatcaacg gta
//
LOCUS       DAZKFU020000397         2650 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZKFU020000397 DAZKFU020000000
VERSION     DAZKFU020000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.399"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..776
                     /locus_tag="ACHOQQ_RS23710"
                     /old_locus_tag="pgaptmp_004742"
     CDS             51..776
                     /locus_tag="ACHOQQ_RS23710"
                     /old_locus_tag="pgaptmp_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4472882.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            734..1411
                     /gene="bglJ"
                     /locus_tag="ACHOQQ_RS23715"
                     /old_locus_tag="pgaptmp_004743"
     CDS             734..1411
                     /gene="bglJ"
                     /locus_tag="ACHOQQ_RS23715"
                     /old_locus_tag="pgaptmp_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGU4472883.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1449..2237)
                     /gene="fhuF"
                     /locus_tag="ACHOQQ_RS23720"
                     /old_locus_tag="pgaptmp_004744"
     CDS             complement(1449..2237)
                     /gene="fhuF"
                     /locus_tag="ACHOQQ_RS23720"
                     /old_locus_tag="pgaptmp_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGU4472884.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDPTLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNVLSSLLAVYSDHIYRNQPMMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQHRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2378..2614
                     /locus_tag="ACHOQQ_RS23725"
                     /old_locus_tag="pgaptmp_004745"
     CDS             2378..2614
                     /locus_tag="ACHOQQ_RS23725"
                     /old_locus_tag="pgaptmp_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGU4472885.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLTYADLSPDKWRIVILMGLLLTP
                     LMLYHKQLRHYVLLPSCLALSAGIMLMLMNLNQG"
BASE COUNT          703 a          581 c          669 g          697 t
ORIGIN      
        1 atgtcctgaa taaaccatca taatttttag acagtggatg tggaggaaat atgttgccag
       61 gatgctgcaa aaatggaatt gttatcagta aaatacctgt tatgcaagca gggttaaaag
      121 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
      181 ttaccttatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      241 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      301 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaactg ctcatgtgcc
      361 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      421 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctccccggca atgactgaaa
      481 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      541 tattaggtaa aggatggggc atcaaccaga tagcttcatt gcttaagaaa agtaataaaa
      601 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      661 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      721 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      781 atgcgtcatg agtagtatcg gtattgagag tttattcaga aagtttgcgg gcaaccctta
      841 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcgtt
      901 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      961 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
     1021 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
     1081 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1141 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgt taagcccgac
     1201 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1261 gcttaaacgc aatattaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1321 cgtcagttct gacgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1381 cgaaacaagt aatgtgttgc atccatatta atccgcatga tgccgggttt acttccccgg
     1441 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1501 tggcagcaag tgcggcgcac cagcaggccg tcgcgtagca ccacggtacg ccagagtgga
     1561 ttatcttcac cggtagtaag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1621 gtcgcctcgc cgagcagttg cttcatctca gtaagatacc agttgatcaa ataaccggta
     1681 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1741 accagcgcct ggctgattaa cgtttccatt cgatgctgcg gcgaatgtgg tgttgcgttt
     1801 ttatcttcac atacatcgac ccagaaacag gcggcgcgtc cggtttcgtg aaactcaacg
     1861 tggaaatgtt ccggcgacac atctaatgct ttttcctgcg tcagcagcgc cagcattaat
     1921 ggcggcacca tcaggccgat ataccactgc gcccacagtg agatcagcgg tttgttctcg
     1981 cggatcatca tcggttggtt gcgatagata tgatcggaat agaccgccag cagagaactt
     2041 agcacattcg gtgatgacca ttgcgccagc gtcatggcgt taagtggggc aggttcatcc
     2101 aggcggataa actcgagcaa atgttcacga tgttttgcga tcgtcgcccg cacggcttgc
     2161 gcaagcgtgg gatcctgcgg ctggagatgc gttcgccaga taacatcttc atagagcggt
     2221 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2281 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2341 gatattggtt atcattatca atgaaagaga taaaaccatg ttgcaacgta tgctgggcag
     2401 tggctgggga gtgttgctgc cgggattgct gattgcagga ctgacgtatg cggatttatc
     2461 gccggataag tggcggattg tgattctgat gggattactg ctgacaccgc tgatgttgta
     2521 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta gtgctggcat
     2581 catgctgatg ctaatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2641 agtaaagata
//
LOCUS       DAZKFU020000398         2639 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZKFU020000398 DAZKFU020000000
VERSION     DAZKFU020000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.400"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(76..873)
                     /gene="mtfA"
                     /locus_tag="ACHOQQ_RS23730"
                     /old_locus_tag="pgaptmp_004746"
     CDS             complement(76..873)
                     /gene="mtfA"
                     /locus_tag="ACHOQQ_RS23730"
                     /old_locus_tag="pgaptmp_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGU4472886.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            967..1056
                     /locus_tag="ACHOQQ_RS23735"
                     /old_locus_tag="pgaptmp_004747"
     tRNA            967..1056
                     /locus_tag="ACHOQQ_RS23735"
                     /old_locus_tag="pgaptmp_004747"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1001..1003,aa:Ser,seq:cga)
     gene            complement(1626..2156)
                     /gene="yodB"
                     /locus_tag="ACHOQQ_RS23740"
                     /old_locus_tag="pgaptmp_004748"
     CDS             complement(1626..2156)
                     /gene="yodB"
                     /locus_tag="ACHOQQ_RS23740"
                     /old_locus_tag="pgaptmp_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4472887.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(2499..>2639)
                     /locus_tag="ACHOQQ_RS23745"
                     /old_locus_tag="pgaptmp_004749"
     CDS             complement(2499..>2639)
                     /locus_tag="ACHOQQ_RS23745"
                     /old_locus_tag="pgaptmp_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HGU4472888.1"
                     /translation="PRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMM
                     SH"
BASE COUNT          764 a          553 c          574 g          748 t
ORIGIN      
        1 ttagcggtac cttgtctgtc aacactaaat ttcataagtc atttcaattg gttaattaat
       61 ctgcaaagtt gttaattaat gaacattcgt cgccgaaaac gagtctgtat cattagcgtg
      121 atgcagtctc tgcaaaggat cttgttgata aaattggcag aaacgttgcc acaatgaagg
      181 aaaacgagga gcaaaaagtt ctggggcgct aaagaaatat tcagaaagta cggcaaaaca
      241 ttcagcagga tcactggcag cataagcatc aatgctcgcc gcattctcgc caaccaattc
      301 gatttcttcc tgaatgttgt tcattgcagc atgaagatcg tgttcccagc cagcaacctc
      361 acgcaacgga ataaagggaa ctccgctggc gcgatcgccg ttacgggtgt ccagcttatg
      421 agcgacttca tgaataatca ggttaaaacc agaagcatca aaagaatctt gtatatccaa
      481 ccagttcaaa acgataggcc cttgctgcca gctctgacct gactgaacaa tacgttggtt
      541 atgcaccaga ccgatatcgt cttcccattc atcatcgacc acaaatggcg caggataaat
      601 taagacttca tgaaaaccat ccagccattc cagtcctaac tccagaacgg gtaggcaaaa
      661 tagaagtgct atccggcagc ttcttaatga atccagttca aagccctgta aaggaacaag
      721 ccgcttttgc tgtaaaaaac gttcggcaag agtgactaat ttgctttgtt cctgttccgt
      781 cagacacgtt aaaaggggga tcgatagtgc ttcctgccag ggaagggcag tttgatgtgc
      841 tgattcttgt actttccagg gccacttaat catcgttttg ctcgcaaact cgtcacttga
      901 acaaaattgc acggacaggg actgttaaaa tgccaaattt cctggcatca tggcaaccat
      961 ctgaacggag agatgccgga gcggctgaac ggaccggtct cgaaaaccgg agtaggggca
     1021 actctaccgg gggttcaaat ccccctctct ccgccacttt atcaatgact tatctcccga
     1081 cttcccgcct tgcttttcct aaacagaaca atcgtagaat attcttgaag ggttagatcg
     1141 tcactgtttt ctgttcgata ctgtgacatt cagcacttga ttcgctatgg atctgacagg
     1201 aagctttcga gcgaaaatgt gcagttattc agtcgttttc ttatcgctca ccattattct
     1261 tttagacatt catcctatga aactgccgca aagttggtgg tgggaactga agttgcgtag
     1321 agaaggggtc aataattgag tcaaaaaagg aacgcggccg tcagattaaa gccgcgggac
     1381 aaagtccata atttccaaga gttgttgaat ttcccagctt ttttgaaagc ctttgtatca
     1441 tactaacaag taaaaaaggt ttaaatacca aaattaacct gtatttttga taagtcttac
     1501 cattgtacga tttacacagt ttctgctcct ccttgttgat agtggaaagg gtgcattgtg
     1561 cacttgcgta aacatttatg acgcgttgat gtccatggtt cacatttcct tgtcagggga
     1621 tcgacttatt tgcggcgagg catcattcgt aacagagtat tatctttctg aatgtagtga
     1681 tgaaagagtg ctgcgccagc gtgagctgcg attaaaaagt aacctatatt tgcccaggtt
     1741 tcgtgaatat tttttatcag tgctttaatt tcgctgtttg gggtaacaaa gggagacaca
     1801 ttgaaaccaa ggaaactcca cgattttcca ctgtaagcca ttaaagcaat ccccagcaga
     1861 ggaagcgcaa ggaaggttat ataaagcatg atgtgcatta gcgaagccgc tttcatttgc
     1921 caggcaggtg gcggtggcac aatagaagga tcactataac ggtgttttat aatcaggcgt
     1981 gaaaacatta acacccaaac gaatataccc gcattgtaat gtgtttctcg catcagcaga
     2041 taagtactac tacctttagg aaaccagcca cggagttcca tcgcggcata ggttattgca
     2101 acgaaaagca gcgttatcca atgtaaataa atttgagttt ttgagaatcg gttcattttt
     2161 gtagctcctg tgattttcac agtcaagcta tcccacgaac cttaaaaatt ccttatacag
     2221 atgtggtttt aagaatttgt tatttaaaaa attgatagtg cacattgtaa ccagccaata
     2281 taagcaatgg cgtgagcaca ggtatctttc tctgttggcc gtattgtgta tggaatccgt
     2341 tattggttaa ctcaatgcgg gttagcttat taatatacag acacgtcata gtgatgagcg
     2401 tgtctgtagc tagcttgcgt tcagtggcgt ttgtggatgg accagtggca gttatgatga
     2461 cttactaaag cggcatcgag gcgttatcat gagaatactc aatgagacat catttcctcg
     2521 accacttctt cgctactcaa ctgatatgga taatacgttg gccagttttc catttcattt
     2581 aataaagatt gctgtgaatc attacccata aaaatgtgaa aatgagatga ttttcgtgg
//
LOCUS       DAZKFU020000399         2630 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZKFU020000399 DAZKFU020000000
VERSION     DAZKFU020000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.401"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(215..767)
                     /locus_tag="ACHOQQ_RS23750"
                     /old_locus_tag="pgaptmp_004750"
                     /pseudo
     CDS             complement(215..767)
                     /locus_tag="ACHOQQ_RS23750"
                     /old_locus_tag="pgaptmp_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            884..1789
                     /locus_tag="ACHOQQ_RS23755"
                     /old_locus_tag="pgaptmp_004751"
     CDS             884..1789
                     /locus_tag="ACHOQQ_RS23755"
                     /old_locus_tag="pgaptmp_004751"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4472889.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGILNTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKK"
     gene            1834..>2630
                     /locus_tag="ACHOQQ_RS23760"
                     /old_locus_tag="pgaptmp_004752"
     CDS             1834..>2630
                     /locus_tag="ACHOQQ_RS23760"
                     /old_locus_tag="pgaptmp_004752"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU4472890.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNV"
BASE COUNT          763 a          440 c          648 g          779 t
ORIGIN      
        1 gcgccagtct tcggactggc gttttgttca actgccagtt ccctgaatgt ttgatttgtt
       61 caataaagcc tgtttatcaa taaattttct gacgacaggg aatggtatgt ttttattagt
      121 ttttatgtgt tggacaacaa gacagaataa tgatgtctgt tttatgtata cgatatagcc
      181 actgattcaa cgcattatcg gctatattgc cagtcttatt tagtgccaga ttcattgtat
      241 cgaaggccgc tgccgtcagt agatggctgg tcagttctat cagacagacc atttttacct
      301 gaggataaag tgtctggttt ccaccatgta tttggcgagt gaagctttat cgtgttcttg
      361 cgtgtcctgt gttcgtcaga acacgccatc aatggtcagc agattcagat cgcaccagag
      421 cggatatggc gtggtgttat gccagagctg cgctgtttga gtgaacacgc agcgtatggc
      481 tccgcttcct ggccgctggc gggcatggag cacggcgcta ctgcctggca gtatgatgtc
      541 cagccggttc acaatctgat gaaggttcct taagctcatg ggccatactg ataattcacc
      601 agaccatcat ttccagagga agacgccgtt tgtgtagcgt gacagtgtcg gactcttcag
      661 ggctgtgggt aatcagttcc agggcaagat agtcgcagag ggttacgcag agaatagtaa
      721 ctggacactg gatcgatcaa ccgatcctta actgagcggc atgacagctt tctgatggat
      781 ttttttgata atcttcacat tgtgcttctt ttctgttgga taatttttgc acttggtggt
      841 gtactaggta tcccattatg cagttaagtg aaggtgatta aaaatgcaat gtgaatttaa
      901 aggggtcata tcagcattac ccactcctta tgaccaatcc cagcaaatcg atatggagag
      961 tcttcgaaaa ctgatccgtt ttaatatcga acaaaatatt aagggattgt atgttggagg
     1021 ctctactgga gaagcatttc ttcagaatgt tgcagagcga gaaaaaattc ttgaaacagt
     1081 agccgatgaa tcagatggaa gactgacact tatagcgcat gtgggcggaa tcagtacggc
     1141 agaaagcgag gtacttgcaa aagctgcaaa aaaatacgga tatcatgcta tatctgctgt
     1201 tactccattt tattatcctt tttcatttga agagcattgt atccattaca ggaagattat
     1261 tgattctgca gatggacttc cgatggtagt ttataatata ccggctctta gcggagtgcg
     1321 tttttcttta gatcagataa atgagcttgt cacaataccc ggggtttgtg cattaaaaca
     1381 gacatccggt gatttattcc agatggaaca aatcaaaagg aatcatccag agcttgtgtt
     1441 atataatggt tacgatgaaa tatttgcatc agggttaatt gcaggtgcag atgggggaat
     1501 cggtagtaca tataatataa tgggatggcg ttatcttgaa atatttgaag cagtcaaaaa
     1561 caatgatgtg attaaggcga aggaaatgca ggtggcctgt aaccaggtta ttgacactct
     1621 tattcagagt ggtgtcctgg caggtataaa aactcttctt tactatatgg ggattctaaa
     1681 tactcctgta tgcagaagtc ctttttctcc ggtaaaggaa aagaatctgg atgtattatc
     1741 aaaacttgca gagcgactgc tcgaagaaca tgacagaaat aaaaaatgaa aataatctga
     1801 ataattgcaa gattgttata taggtgaaaa tgaatgataa cactggctgt tgatatagga
     1861 ggaacgaaaa tttcagcggc gttgatatct gatgacggtt catttttgct gaaaaaacag
     1921 atatcaacac ctcatgaaag atgtcctgac gaaatgacag gggctttacg tctactggtt
     1981 tctgaaatga agggaacagc ggagcgattt gcagtagcat caacagggat tattaataat
     2041 ggagtattga cagctctgaa tccagacaat cttggagggc tgaaagagta tcctctgaaa
     2101 aatataatgg aagatataac tggtttaaat ggaagtgtga taaatgatgc ccaggcagcc
     2161 gcatgggcag aatatacagt tttgccaaaa gaaatttgtg atatggtttt tataaccgtt
     2221 tctacggggg ttggaggggg aattgtagtt aacaggaaat tattaacagg tgtttctgga
     2281 ttagccggac atgttgggca tatattgtcc ggagtaacgg atacagaatg tggatgtggt
     2341 cgtcggggat gcgttgaagc ggtttcatct ggtcgggcca tcatgggggc agcaaaaaat
     2401 gaacttgcag gatatagcac caaatatatt tttgaattag cgagacaggg atataaggaa
     2461 gcagaatttt tgacagagcg ttctgccagt actattgctg aattaatagt aagtttaaaa
     2521 ttactgcttg attgtcaggt tgttgttgtc ggaggaagtg ttggacttgc tgatgggtat
     2581 attcagaaag tgtcgaaaca tctttccata tattctgaaa tttgtaatgt
//
LOCUS       DAZKFU020000400         2590 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZKFU020000400 DAZKFU020000000
VERSION     DAZKFU020000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.402"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            238..432
                     /locus_tag="ACHOQQ_RS23765"
                     /old_locus_tag="pgaptmp_004753"
     CDS             238..432
                     /locus_tag="ACHOQQ_RS23765"
                     /old_locus_tag="pgaptmp_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4472891.1"
                     /translation="MSTKNKTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            792..1085
                     /gene="iss"
                     /locus_tag="ACHOQQ_RS23770"
                     /old_locus_tag="pgaptmp_004754"
     CDS             792..1085
                     /gene="iss"
                     /locus_tag="ACHOQQ_RS23770"
                     /old_locus_tag="pgaptmp_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HGU4472892.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(1176..1358)
                     /gene="rzoD"
                     /locus_tag="ACHOQQ_RS23775"
                     /old_locus_tag="pgaptmp_004755"
     CDS             complement(1176..1358)
                     /gene="rzoD"
                     /locus_tag="ACHOQQ_RS23775"
                     /old_locus_tag="pgaptmp_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HGU4472893.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1575..2072)
                     /gene="rrrD"
                     /locus_tag="ACHOQQ_RS23780"
                     /old_locus_tag="pgaptmp_004756"
     CDS             complement(1575..2072)
                     /gene="rrrD"
                     /locus_tag="ACHOQQ_RS23780"
                     /old_locus_tag="pgaptmp_004756"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU4472894.1"
                     /translation="MPPSLRKVVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(2072..2287)
                     /gene="essD"
                     /locus_tag="ACHOQQ_RS23785"
                     /old_locus_tag="pgaptmp_004757"
     CDS             complement(2072..2287)
                     /gene="essD"
                     /locus_tag="ACHOQQ_RS23785"
                     /old_locus_tag="pgaptmp_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HGU4472895.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          742 a          628 c          553 g          667 t
ORIGIN      
        1 attttcataa atagcgaaaa cccgcgaggt cgccgccccg taacctgtcg gatcgccgga
       61 aaggacccgc aaaatgataa taattatcat ctatatgtca caacgtgcat ctacgccatc
      121 aaaccacgtc aaataatcaa ttatgacgca ggtatcgtat taattgatct gcatcaactt
      181 aacgtaaaaa caacttcaga caatacaaat cagcgacact gaatacgggg caacctcatg
      241 tcaacgaaga acaaaacccg cagaacaaca acccgcaaca tccgctttcc taaccaaatg
      301 attgaacaaa ttaacatcgc tcttgagcaa aaagggtctg ggaatttctc agcctgggtc
      361 attgaagcct gccgtcggag actaacgtca gaaaagagag catatacatc aatccaaagt
      421 gatgatgaat aaacatcccg gtttcttcca ccatcgcacc ggaaaagcga ctatgagggt
      481 aaccctgcgt ctgtcagcac agtaaaaccc ggtgtgcatc gtttttgatt attcccgcac
      541 actcacgcag aaggaattcc ccgtcgggct acggtcatgg ttaatgcggg aatacggcga
      601 cgatacagcg cagctaaaag ggtaatggac agatagagcg gtttatttca ttccacagga
      661 ttctgagtgc ccccctcctc caataggctg agcatccacc tatatagttt taattttcat
      721 caatccattt aactatcgtt taattgttgt cacataggat tctgccgttt ttaacaatgc
      781 aggataataa gatgaaaaaa atgttgtttt ctgccgctct ggcaatgctt attacaggat
      841 gtgctcaaca gacgtttact gttggaaaca aaccgacagc agtaacacca aaggaaacca
      901 tcacccatca cttcttcgtt tcgggaattg gacaggagaa aactgttgat gcagccaaaa
      961 tttgtggcgg cgcagaaaat gttgttaaaa cagaaaccca gcaaacattc gtaaatggat
     1021 tgctcggttt tattacttta ggcatttata ctccgctgga agcgcgtgtg tattgctcac
     1081 aataattgca tgagttgccc atcgatatgg gcagctctat ctgcactgct cattaatata
     1141 cttctgggtt ccttccagtt gtttttgcat agtgatcagc ctctctctga gggtgaaata
     1201 atcccgttca gcggtgtctg ccagtcgggg ggaggctgca ttatccacgc cggaggcggt
     1261 ggtggcttca cgcactgact gacagactgc tttgatgtgc aaccgacgac gaccagcggc
     1321 aacatcatca cgcagagcat cattttcagc tttcgcatca gctaactcct tcgtgtattt
     1381 tgcatcgagc gcagcaacat cacgctgacg catctgcatg tcagtaattg ccgcgttcgc
     1441 cagcttcagt tctctggcat ttttgtcgcg ctgggctttg taggtaatgg cgttatcacg
     1501 gtaatgatta acagcccatg acaggcagac gatgatgcag ataaccagag cggagataat
     1561 cgcggttact ctgttcattg ctgaccccac aaacagattt cacgctcaat ctcacgacga
     1621 gtcatgagac ctttccattg cttaccgcca gcatatgtcc agcgacgtag ctgatcacat
     1681 gcgcctttga tatcgccctg gtttattttg cgaagaagcg tcgatgttct gaaattgcca
     1741 gcacccacgt tgtaaacgaa tgagtaaaga gcgccgcgcg ttgtttccgg tatatcgact
     1801 ttgatgtacg ggttaatttg tctggcgaca gtggcaaggt ctttattcaa gagtgctttg
     1861 cattctgctt tggtatacgt tttaccgagc atgatgtctt ttcctgtatg cccgtgacat
     1921 acagtccata caccaacaat atctttgtat ggtatgtagc tgacaccttc cagaccatcg
     1981 ttaccacttg ggccagtgat taacactgat gctatagcaa ttgctccgcc accaatagca
     2041 gcagcaacga cttttcgtaa tgatggaggc attattcacc tctcgcagcc ttgcgcttat
     2101 cttctttaat cttgaaataa aggtttgtca ggtacgtcag caggccaaat accaggctac
     2161 ccagcacacc tattgctgcc cactgtgagg gcgtgacttt atcgagcagc tgtaaaaacc
     2221 agtaaccggc actacctgct gaggtgccat aggcgacacc cgttgttaac ttatccatgg
     2281 atttcataac cccacctcgc agacaaagcg ggtgtaaatt gagggaatac aacgtatcgc
     2341 aaaaaagcag aaacgtaaca gactcggagt cagtgaataa cttaggtatt gagttatcag
     2401 ctaatatcga gactcaaaaa atggaaaaac cagctcgacg gcgggtttaa gctgtgtgac
     2461 gaagtaacca ctcttaacag cataaccaat tttttacgta cgtaaaccac tgaatgatat
     2521 ttatgagaat gctaccgagt gttcaaaaca ccaccacaaa tacataagaa aacctcaaca
     2581 aataaccaat
//
LOCUS       DAZKFU020000401         2586 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZKFU020000401 DAZKFU020000000
VERSION     DAZKFU020000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.403"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(102..956)
                     /gene="rclR"
                     /locus_tag="ACHOQQ_RS23790"
                     /old_locus_tag="pgaptmp_004758"
     CDS             complement(102..956)
                     /gene="rclR"
                     /locus_tag="ACHOQQ_RS23790"
                     /old_locus_tag="pgaptmp_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGU4472896.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGMDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQFAP"
     gene            1182..2507
                     /gene="rclA"
                     /locus_tag="ACHOQQ_RS23795"
                     /old_locus_tag="pgaptmp_004759"
     CDS             1182..2507
                     /gene="rclA"
                     /locus_tag="ACHOQQ_RS23795"
                     /old_locus_tag="pgaptmp_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HGU4472897.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGVAVNERGGIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLIK"
BASE COUNT          737 a          581 c          599 g          669 t
ORIGIN      
        1 aaatatataa agcccaaacc accgttggaa atagaaatag ttgaaaggct cacaactccc
       61 ggaatgcgga ttatctttgc cctgatttct ggcggtttta ttcagggtgc aaactgtctg
      121 accctttccc gatattctcc cggagtacaa ccaaactcgc ggacaaacgc cttgtgaaaa
      181 gatgattcac tggcatagcc tactgactca gcgatcacca caacagggag catttcccgg
      241 gaaaacatct gggccgctat ttgtagacgc aactttgtta atacagccag cggcgtggtt
      301 ccggaaacat cacggaaaag ctgggcaaaa cttgcccggg acatgtgggc gatgctggcc
      361 agcgattcga cggtccaggc gtgtcctggc atttccagca tttgctgtat taccgcacca
      421 agacgtggat gcagaagcaa actgagaatg tttttctcag tattaacctg tgcaatccac
      481 tcacgcaccg cgagggtaaa gaatgtagcg cagatctggc tacacagagc atccattccc
      541 ggcattgccg atctggattc ctgttgtaaa aacgggatcg cctccctcag ccagttatat
      601 tccacgctgt ggttaaccgg tgctaaaaac agcgtttccg gcaaagacgt taaaaaataa
      661 cgcgccgaat gttgcagccg aagagtgccg cagacaatac aggtcgattc attatcgaca
      721 tgactcaggc gatgagctga attttgtggt agcaggacca catttcccgg gcgtaatgta
      781 aaaatctctc ccgtcggcat ttccagcttc gccgctcctt gcgttaacgc atgccaacga
      841 ataaccgaca attccccggc accatgtgga agctgccagt cacttcctaa cacgcaattc
      901 ttatcgatcg ttccttgtgg agcgttaagc atcaacaaac ggctaagggc atccatatca
      961 gactcctgag tggtttttga ttacaaacac caacaatctt agtaaacaac taatctaata
     1021 ccatcaaata gttaatcatg atttttgttt tgtgattctg attaagaaaa agaaaatacc
     1081 agacgataag acaaaatatc aagcgttgtg aagaaatgtt atttgctctt ttgcgtctat
     1141 agtcatgatg tcaaatgaac gcgtttcgac aggaaatcat catgaataaa tatcaggcag
     1201 tgattattgg ttttggcaag gctggaaaaa cattagccgt cacgctggca aaagcaggtt
     1261 ggcgtgtggc tctcatcgaa caatcaaatg caatgtatgg cgggacctgt attaatatcg
     1321 gctgcatccc aaccaaaaca ttggttcatg acgcacagca gcacacagat tttgtccgtg
     1381 ccatacagcg taaaaatgaa gtggttaatt ttttacgtaa taagaatttt cataatcttg
     1441 cagatatgcc caatatcgac gtgatcaacg gccaggcgga gtttatcaat aatcatagcc
     1501 tgcgtgttca tcggcctgag ggaaatctgg aaattcatgg cgagaaaatt tttattaata
     1561 ccggtgcaca aaccgtggtt ccgccaattc ctggaattac caccacgcct ggtgtatatg
     1621 acagcaccgg attacttaat ctaaaagaat tgcctgggca tttaggtatt ttgggcggcg
     1681 gatatattgg cgttgagttc gcctctatgt tcgctaattt tggcagcaag gtaaccattt
     1741 tagaagcagc ttcgctgttt ttgcctcggg aagatcggga tattgctgat aatatcgcga
     1801 cgattttacg cgatcagggc gtcgatatta tcctcaatgc ccatgtggag cgaatcagtc
     1861 accatgaaaa tcaagtgcaa gtgcatagcg agcacgccca actggcggtg gatgcactgt
     1921 taatagcttc cggtcgtcaa ccggctaccg cttcgttaca tccagaaaat gccggtgtcg
     1981 cagtaaacga gcgcggggga attgtcgttg acaagcgatt acataccacc gcagacaata
     2041 tttgggcgat gggagatgtt accggcgggc tgcaatttac ttacatatca ctggatgatt
     2101 accgcattgt acgtgatgag ttactgggtg aaggcaaacg tagtactgat gatcggaaaa
     2161 atgtgcctta ttccgtattt atgacaccgc ccttgtccag ggttggtatg acagaagaac
     2221 aagccagaga gagtggtgct gatattcagg tggtgacatt gcctgtagct gcaattccgc
     2281 gcgccagagt gatgaacgat acccgtgggg tattaaaagc gattgttgat aataaaaccc
     2341 aacgtatatt aggggcatca ctgctgtgtg ttgactccca cgagatgatt aatatagtga
     2401 aaatggtgat ggatgccggg ctgccttata gcatattacg cgatcagata tttactcatc
     2461 cgtcgatgag cgaatcactc aatgatctat tttcattaat caaataaact caaaatcaga
     2521 cgccagaaca aatattctgg cgtcccagag aaaagaatct tattaattcc ctgttactct
     2581 ataact
//
LOCUS       DAZKFU020000402         2564 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZKFU020000402 DAZKFU020000000
VERSION     DAZKFU020000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.404"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..865
                     /gene="gatY"
                     /locus_tag="ACHOQQ_RS23800"
                     /old_locus_tag="pgaptmp_004760"
     CDS             11..865
                     /gene="gatY"
                     /locus_tag="ACHOQQ_RS23800"
                     /old_locus_tag="pgaptmp_004760"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGU4472898.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKKYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            894..2156
                     /gene="gatZ"
                     /locus_tag="ACHOQQ_RS23805"
                     /old_locus_tag="pgaptmp_004761"
     CDS             894..2156
                     /gene="gatZ"
                     /locus_tag="ACHOQQ_RS23805"
                     /old_locus_tag="pgaptmp_004761"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HGU4472899.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRTYRYGCAE"
     gene            2166..>2564
                     /gene="gatA"
                     /locus_tag="ACHOQQ_RS23810"
                     /old_locus_tag="pgaptmp_004762"
     CDS             2166..>2564
                     /gene="gatA"
                     /locus_tag="ACHOQQ_RS23810"
                     /old_locus_tag="pgaptmp_004762"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGU4472900.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLP"
BASE COUNT          665 a          595 c          668 g          636 t
ORIGIN      
        1 ggatatgaaa atgtacgtgg tatcgacaaa gcagatgctg aacaacgcac agcgcggcgg
       61 ttatgcggtt ccggcattca atattcacaa tctcgaaacg atgcaagtgg tggtagaaac
      121 cgctgccaac ctgcatgcgc cggtcatcat cgccggaacg cctggcacat ttactcatgc
      181 tggtacagaa aatctcttgg cgctggtcag cgcgatggcg aagcaatatc accatccact
      241 ggcaattcat ctcgaccatc acacgaaatt tgacgatatc gctcagaagg ttcgttctgg
      301 cgtgcgctca gtcatgattg acgcctcgca tttgcctttt gcgcaaaata tttcacgggt
      361 caaagaggtg gtggattttt gccatcgctt tgatgtcagc gtcgaagcgg agctggggca
      421 acttggcggc caggaagatg atgtgcaagt caatgaagcc gatgcttttt ataccaaccc
      481 cgctcaggcg cgtgaatttg ccgaggcaac cggaattgat tccctggcgg tcgccatcgg
      541 cacggctcat ggtatgtatg ccagcgcacc ggcgcttgat ttttctcgac tggagaacat
      601 tcgccagtgg gtgaacttac cgctggtgct gcatggcgcg tcagggttat cgactaagga
      661 tattcagcaa accatcaaac tggggatatg caaaatcaac gttgcaacgg agctgaaaaa
      721 tgccttctcg caggcgttaa aaaaatacct gaccgagcac cccgaagcga ccgatccccg
      781 ggattatttg cagtcggcta aatccgcaat gcgcgatgtg gtgagcaaag tgattgccga
      841 ttgtggctgc gagggcaggg cataacgcac ctgccattta acaaggaaaa aacatgaaaa
      901 cgttaattgc ccggcataaa gctggtgaac atatcggcat atgttcagtc tgttctgccc
      961 atccgttggt tatcgaagcg gcgctggcat ttgatcgcaa cagcacgcgc aaagtgctga
     1021 ttgaagcaac gtcaaaccag gtcaatcaat ttggcggtta taccggaatg acaccggcag
     1081 actttcgcga atttgttttt acgattgccg ataaagtcgg atttgcgcgc gagcgcatta
     1141 ttctcggcgg agaccatctg gggccaaact gctggcagca agaaaatgcg gatgcggcaa
     1201 tggaaaaatc cgtcgagctg gtaaaggcat atgttcgtgc tggcttcagt aaaattcatc
     1261 ttgatgcgtc aatgtcctgc gcgggggatc ccataccgtt agcaccagaa acggttgcgg
     1321 aacgagctgc tgtgctttgc tttgctgcgg aaagtgtggc gacagattgc cagcgtgagc
     1381 aactgagcta tgtcattggc accgaagttc cggttccggg cggtgaggcc agcgccattc
     1441 agtcagtaca catcacccat gttgaagatg ccgccaatac tttacgtacg catcaaaagg
     1501 cctttattgc ccgtggcctg acagaggcgt taacacgtgt gattgccatc gtggtgcagc
     1561 cgggtgtgga atttgatcac agcaatatta tccattatca gccgcaggaa gcgcaggcgc
     1621 tggcgcaatg gatagaaaac acccgaatgg tttatgaagc acattctacc gattaccaga
     1681 cccggacggc ttattgggaa ttagtccgcg atcactttgc aatattgaaa gtcggtcccg
     1741 cattaacctt tgctttacgc gaggcgatat ttgcactggc acaaattgag caggaactta
     1801 tcgcccccga aaatcgcagc ggttgcctgg cggtaattga agaagtgatg ctcgacgaac
     1861 cgcaatactg gaaaaaatat tatcgcacgg gttttaacga ttcattactg gatattcgtt
     1921 acagcctatc ggatcgtatt cgttattact ggccgcatag tcggattaaa aatagcgttg
     1981 aaacgatgat ggtgaatctg gaaggcgtgg acatcccact gggcatgatt agtcagtatc
     2041 ttcctaaaca atttgaacgc attcagtccg gggaattatc agcaataccg catcagctta
     2101 ttatggataa aatttatgat gttttgcgca cctatcgcta cggctgtgcg gaataaggac
     2161 ggtatatgac taacctgttt gttcgtagcg gaatttcttt tgtcgatcgt agcgaagttt
     2221 taacccatat cggtaatgag atgctcgcga aaggtgtggt tcatgatacc tggccacagg
     2281 cattaattgc cagagaagca gaattcccta ctgggataat gcttgagcag cacgccattg
     2341 caataccgca ttgtgaggcg attcatgcta agtcgtcagc catttatctg ttaaggccaa
     2401 caaataaagt tcattttcag caagcggatg atgataatga cgtggcggta tcgttggtta
     2461 ttgcgttgat tgtggaaaat ccgcagcagc aattgaaact tttacgctgt ttatttggca
     2521 agttacaaca gcccgatatc gtcgagacac taatcactct tcct
//
LOCUS       DAZKFU020000403         2552 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZKFU020000403 DAZKFU020000000
VERSION     DAZKFU020000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.405"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..1268
                     /locus_tag="ACHOQQ_RS23815"
                     /old_locus_tag="pgaptmp_004763"
     CDS             3..1268
                     /locus_tag="ACHOQQ_RS23815"
                     /old_locus_tag="pgaptmp_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HGU4472901.1"
                     /translation="MKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESATLPV
                     ITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLTLVL
                     PASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVLSLD
                     MAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAETWF
                     DRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQLYR
                     DYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSPILF
                     TENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTVYTG
                     GHDYAWWRGAIIDELANYNCRKVSNNNSV"
     gene            complement(1410..2534)
                     /locus_tag="ACHOQQ_RS23820"
                     /old_locus_tag="pgaptmp_004764"
     CDS             complement(1410..2534)
                     /locus_tag="ACHOQQ_RS23820"
                     /old_locus_tag="pgaptmp_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HGU4472902.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIHFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          778 a          575 c          485 g          714 t
ORIGIN      
        1 ctatgaaaaa ctatcttttc cagacacccg aagatatttg tgtacagtta aaaaaaatga
       61 cacatcctgt cacaataaga acaacagata ttgctaattt ctggcactat cttgagtcag
      121 caacacttcc agtgatcaca aaaagcacca ctacagaaaa tcgggaggtt acatttctgt
      181 ggcgctcaga gaaagcactg caaggcgtat atcttcgcct gaatcgtgta acagataaaa
      241 aagacgtcaa aaaaggacta atgactcata tcccttcgac agatatctgg atgctgacac
      301 tggtgttacc agcttcatat cggggctcat actcatttat agaaattccc acagatatga
      361 cacaaaaaga catatttcaa ctaggaagtc gcttctctcc attacccggt aaatctgatc
      421 catttaacaa aacagcagaa ataaatatac gaggattcgg agaatcagtc ctttctcttg
      481 atatggctcc tgaacaaaag gaatgggatg atacttccca taaatgtaca ggtattcttt
      541 caacattaca ttcctttgtt gcaggatatc aacgccggat tcgtttatat tttcccctga
      601 atccaacatc agtacctctt ggattacttg tgttacctga tgctgaaaca tggtttgacc
      661 ggatagatat tacccgggca ttagatatgg ccatcaccac tggtcgtatc acgccaatgg
      721 caattatggg gatagacaat attaatgaat ctgatcgtat gaatatactg ggaggcaata
      781 aagaactcat ctttgatata gcggaaaatc tgatacccca gttatacaga gactatccgg
      841 atatcgtatg ggctggtcgc tctaatactg tactggccgg gcagagcctc ggtggagtga
      901 cagcactgat ggcagctata tatgcgtcga caacatttgg tacaatcatt agtcactcac
      961 cttcaatgtg gtggaaccct gaccagggca gcccgatctt gtttactgag aataatacct
     1021 cctgggtaag tgagcagata ctttcagcgc ctccgaaaga tgtaaatatt cgacttggag
     1081 tcggttcttt agaaggtaca accgtctcac atgttcagcg attgcatcat tcgttaatcg
     1141 cagctggttt ggaaagtaac ctcactgtct ataccggtgg tcacgattat gcttggtggc
     1201 gcggagcaat tattgatgaa ttagcaaatt ataattgcag gaaggtatca aataataact
     1261 ctgtgtaaaa tatcttttta tcgtccgata actctttctg caatattatt atgggagact
     1321 atctcattta tttagtgatg tccttcattt atgtgatgaa ggacattata ttactcataa
     1381 catttctcgt tgaaaatttc agcaatacgc tacaattgtt tcccgttgtt ttttaatttt
     1441 ctcatcaaaa cctctgcaat ctctgcagga ctgggttgtt cagccatttc agcggctacc
     1501 tgatctgaac agaagcgtag tgaatcatca taaagcaggc gatttaccaa atcactagtc
     1561 agtccatgct tgtccggaat aatcccacac ccacgcttcg caacaatttc tgcattaaca
     1621 gagcgatctg caccttcgcc aaacacaatc tgtggtattc cactatacag cgctgtcagg
     1681 gtattacctg cgccaccgtg atgaataaat ccatcagctc cattaaggaa tacacccata
     1741 ggtatccagt caaccagacg tacatttgat ggtagttttc gtaaaccagt acgagcattt
     1801 attgcaagtt gcaaaatgat atcagcatca acttcatttg cagaatccat aacccatgaa
     1861 atcagctcca gaccatcaac cattggtttt acagtgccaa ggctgataag taaacgtttc
     1921 cgatcagaat ccctgtccca ccatgtttcc tttacagcac ctccgttata aggaatatat
     1981 ctcattgaga taaccggttc tccagcattt ttaagaatgc tcatacttgg gggagcaaca
     2041 tcaatccatg ctaaatcaca tagtggaccg ctgactccat gccgcctgta agcattgctt
     2101 aaagaacggt ttaacatctg aatatgggca gatgtatgcg cgaatccaac agccagcatc
     2161 actgaaggaa ttctatattt agcagcaacc aatgggcctg ccggaccaag agggggatag
     2221 actattaaat ctggcctcca ttttcctgca aaatggatga ggttatctgc catttcatcg
     2281 ctaaaaaaag agaaatgacc aataatatta ctttttttcc tcaactcttc ctgatggcga
     2341 tatccagcct ctgaatctaa acctggaact gcatcaaaaa caaccagtcc agcttctgct
     2401 gctttattcg cgaatttgcc agcgctacta attactacat catgtccaat tacacgaaag
     2461 gcctgagcca gagaaatcaa tgggtataac aaaccgtaca gcggtgggcc aataaaaaga
     2521 atacgttttc gcattaaaaa agtccgtctt ct
//
LOCUS       DAZKFU020000404         2529 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZKFU020000404 DAZKFU020000000
VERSION     DAZKFU020000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.406"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>2529)
                     /locus_tag="ACHOQQ_RS23825"
                     /old_locus_tag="pgaptmp_004765"
     CDS             complement(<1..>2529)
                     /locus_tag="ACHOQQ_RS23825"
                     /old_locus_tag="pgaptmp_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HGU4472903.1"
                     /translation="ASTMTTKPDGTVEISVTSQTAGTSTVTASINNSSQSRDVTFIAD
                     VRTAQIADLVVIKDSSEADGATANTLRARVTDAFGNALAGQTVSVLADNGATVSPTVT
                     TEPDGTVEISVTSQTAGTSVVTVSINNSSLSRNVTFIADVRTAKIADLAVTQDGSVAD
                     GATANTLRVRVTDAFGNTLAGQTVSVLADNSATVTPTVITGQDGTVEISVTSQTAGTS
                     VVTASINNSSLSRDVTFIADIRTAQIADLVVIKDGSEADGATANTLRARVTDAFGNAL
                     AGQTVSVLADNGATVSPAVTTEPDGTVEISVTSQTAGISTVTATINSSSLSRNVTFIA
                     DVRTAQIADLVVIKDGSVADGSTANTLRVRVTDAFGNALGGQTVSVLADNGATVASTV
                     TTGQDGTVEISVTSQTAGVSAVTATINNSSLSQSVMFIADVRTAQIAELVVIKDGSEA
                     DGATANTLQVTVTDAFGNTLAGQTVSVLAGNGATVAPTVITEPDGTVEISVTSQTAGI
                     STVTASINSSSLSRNVTFIADVRTAKIADLVVIKDDSVADGAMANMLQVKVTDAFGNA
                     LNGQTVSVSADNSAMVTPTVTTQPDGTVEISVTSQTAGISTVTATINNSILSRNVMFV
                     ADVRTAQIADLVVIKDDSVADGAMANILRARVTDAFGNTLAGQTVSVMADNGATVALT
                     MTTKPDGTVEISVTSQTAGISTVTATINNSTLSQNVTFIADIRTAQIADLVVIKDDSV
                     ADGAMANMLRARVTDAFGNALAGQTVSVFAGNGATTAPTVTTQPDGTVEISVTSQTAG
                     TSAVTASINSSSQNRDVTFIADVRTAKIASLEVTQDNAVADGAMA"
BASE COUNT          413 a          876 c          661 g          579 t
ORIGIN      
        1 cgccattgcg ccgtcagcca cggcgttatc ctgcgtcacc tccagacttg cgatcttcgc
       61 cgttctgaca tcggcgataa acgtcacatc ccggttctgg ctgctgctgt taatgctggc
      121 agtgaccgca ctggtgcctg ccgtctggct ggtgacagag atctccaccg tgccgtccgg
      181 ctgcgtggtg accgtcggag cagtcgtggc accgttgcct gcgaacacgc tgaccgtctg
      241 cccggcaagt gcattaccga acgcatcggt gaccctcgcc cgcagcatat tcgccattgc
      301 gccgtcagcc actgagtcgt ccttaatgac caccaggtcc gcgatctgcg ccgtcctgat
      361 atcggcgata aatgtcacgt tctgactcag ggtgctgttg ttgatggttg cagtgactgt
      421 actgatcccc gccgtctggc tggtgacact gatctccacc gtgccgtccg gctttgtggt
      481 catcgtcaaa gcgaccgttg caccgttgtc cgccatcacg ctgaccgtct gcccggcaag
      541 cgtattaccg aacgcatcgg tcaccctcgc ccgcagaata ttcgccattg caccgtctgc
      601 cactgagtcg tccttaatga caaccagatc agcgatctgc gccgtcctga catccgcgac
      661 aaacatcacg ttccggctca ggatgctgtt gttgatggtt gcagtgactg tactgatccc
      721 cgccgtctga ctggtgacac tgatctccac cgtgccatcc ggctgcgtgg tcaccgtcgg
      781 agtgaccatt gcgctgttgt ctgccgatac gctgaccgtc tgcccattca gcgcattccc
      841 gaatgcatcg gtgaccttca cttgcagcat attcgccatt gcaccgtctg ccactgagtc
      901 gtccttaatg actaccagat cagcgatctt cgccgtcctg acatccgcaa taaacgtcac
      961 gttccggctc aggctgctgc tgttgatgct ggcagtgact gtactgatcc ccgccgtctg
     1021 gctggtgaca ctgatttcca ccgtcccgtc cggctctgta atcaccgtcg gggcaaccgt
     1081 tgcgccgttg cctgccaaca cgctgaccgt ctgcccggca agcgtattac cgaacgcatc
     1141 ggtgaccgtc acctgcagcg tattcgccgt cgcaccgtct gcctctgaac catccttaat
     1201 aaccaccagc tccgcgatct gcgccgtcct gacatcggca ataaacatca cgctctggct
     1261 caggctgctg ttgttgatgg ttgccgtgac tgcactgact cccgccgtct ggctggtgac
     1321 tgagatctcc accgtgccgt cctgccctgt ggtgaccgtc gaagcgaccg ttgcgccgtt
     1381 gtccgccaac acgctcaccg tctgcccgcc cagcgcatta ccgaacgcat ccgtcaccct
     1441 cacccgcagc gtgttcgccg tcgaaccgtc tgccactgag ccatccttaa taaccaccag
     1501 atcagcgatc tgcgccgtcc tgacatcggc gataaacgtc acgttccggc tcaggctgct
     1561 gctgttgatg gttgcagtga cagtactgat ccccgccgtc tggctggtga cactgatttc
     1621 caccgtgccg tctggttctg tggtgaccgc cggagacacc gttgcgccgt tgtccgccaa
     1681 cacgctgacc gtctgcccgg caagcgcatt gccgaacgca tcggtcaccc tcgcccgcag
     1741 cgtgttcgcc gtcgcaccgt ccgcctctga accatcctta atgaccacca gatccgcgat
     1801 ctgcgccgtc ctgatatcgg cgataaacgt cacatcccgg ctcaggctgc tgttgttgat
     1861 gctggcagtg accacactgg tgcccgccgt ctggctggtg acactgattt ccaccgtgcc
     1921 gtcctgccct gtgatgacag tcggagtgac cgttgcgctg ttatccgcca acacgctgac
     1981 cgtctgcccg gcaagcgtgt tcccgaacgc atccgtcacc ctcactcgca gcgtgttcgc
     2041 cgtcgcgcca tctgccactg agccgtcctg cgtcaccgcc agatcagcga tcttcgccgt
     2101 cctgacatcg gcaataaatg tcacgttccg gctcaggctg ctgttgttga tgctgacagt
     2161 gaccacactg gtgcccgccg tctggctggt gacactgatc tccaccgtcc cgtccggctc
     2221 tgtggtaacc gtcggagaca ccgttgcgcc gttgtccgcc aacacgctga ccgtctgccc
     2281 ggcaagcgca ttgccgaacg catcggtcac cctcgcccgc agcgtattcg ccgtcgcacc
     2341 gtccgcctct gaactatcct taatgaccac cagatccgcg atctgcgccg tcctgacatc
     2401 cgcgataaat gtcacatccc ggctctgact gctgttgttg atgctggcag tgactgtact
     2461 ggttccggcg gtctggctgg tgactgagat ctccaccgtg ccgtccggct ttgtggtcat
     2521 cgtcgaagc
//
LOCUS       DAZKFU020000405         2528 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZKFU020000405 DAZKFU020000000
VERSION     DAZKFU020000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.407"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..561
                     /locus_tag="ACHOQQ_RS23830"
                     /old_locus_tag="pgaptmp_004766"
     CDS             <1..561
                     /locus_tag="ACHOQQ_RS23830"
                     /old_locus_tag="pgaptmp_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU4472904.1"
                     /translation="IDAINKIKIELHRSSPFYAEPVDCVIWIKNNLIEANDYNPNVMA
                     PSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGFHRQMLGKSTTKLEKKLKGYLPVT
                     CIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIVRDLSRMGWDDLRIGSELGMDTDE
                     VLRLKQISGLTELFEEENFSPAWTIK"
     gene            complement(743..2488)
                     /locus_tag="ACHOQQ_RS23835"
                     /old_locus_tag="pgaptmp_004767"
     CDS             complement(743..2488)
                     /locus_tag="ACHOQQ_RS23835"
                     /old_locus_tag="pgaptmp_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4472905.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMAVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
BASE COUNT          760 a          626 c          516 g          626 t
ORIGIN      
        1 attgatgcaa ttaataaaat aaagattgag ttacatagga gtagtccttt ttacgcagaa
       61 cctgttgatt gtgttatatg gataaaaaac aatctaattg aagcaaacga ctataatcct
      121 aacgtcatgg ctccctccga aaaaaggtta ttaacacact ccctgttggt tgatggattc
      181 actcaacctg ttgtcacctt agctcaaaag gggaaataca ccgttattga cggatttcac
      241 agacaaatgc ttgggaaaag cacaacaaaa ctagagaaaa aactaaaagg atatttacct
      301 gtcacatgca ttaagcatga taataacttt aaagcttact ctatcgcctc gaccatcagg
      361 cataacaggg ccagaggaaa gcaccagata gacgcgctct ccgatatcgt ccgagatctt
      421 tcccggatgg gatgggatga tctccgaata ggtagtgaac tgggcatgga tacagatgag
      481 gtactgagat taaaacaaat aagtgggtta acagaactat ttgaggagga aaactttagt
      541 ccggcatgga caataaagtg atcatttagg gggtgacttt atccagtaat taccgactga
      601 tatatgatca ccccatgtaa tggacagcta ttgacagatc aaaaacatgg ggaatattaa
      661 ttcaactctg aatactcgct aaatacccat cttagattat caaaaaacga atgattacat
      721 atcagcaata ataaaacaca acttatacaa aatcctcaag atgacgactg atactgtttt
      781 cacgataaca ggtcaccagt aattcccgtg tagcccgggt gaacgcaaca taaagcgccg
      841 gaataacttc agactcatcc gcagcacctg agtgaacgaa agaagcattg ataaccgcaa
      901 catacggaaa ctccagtccc ttactgctct ggtaggtcag taaatgcaca acatccttgc
      961 gtcgcgaata tttcttctta ccttcactgt caaagcaggt ggcataaggt atcccgcgtt
     1021 gtgtcatgac ctctttcaga tgtttcactg aaaactctgc aggacacaaa acagccatat
     1081 cactccagtg accgcacagt gtatagcgtt tctccagcca gcccacaacc tgcactgcct
     1141 catcactctc agaagcacaa cgcagaattt ccggctctgt gccctcttca ccgcccgcct
     1201 gtggctgaac aaacggtatt tcacggttct gatgcttctc aaaatactca cgggagaatg
     1261 cataagcaaa gtgcagaatg cgtttcgtat tacggtaatt taccggcagg acggaagtac
     1321 gtccctgcgc ctgaatcccc acactggcaa gcgaaaagtt cagagcccgt tcccgtctgt
     1381 aaatggactg tgcgtcatcg tacatcagga gcagtgaacg ggacgcgtta tcaaacaggc
     1441 gggctatcag agccagccag cgactctcaa aatcatgccc ctcatccaca agtacggcat
     1501 cgtatccagt atcagtaatt gttccgctgt tcacggcatc ttccagcgca ctgaagcatt
     1561 tctccgggta atcctttcct ccagcagtca cctgaattcc atggcgcttt atcattgagg
     1621 cgcaccaact atgaaaatgg aataccgtaa cccgtgactt cagtcccctg gactcaatac
     1681 attcccgcag gtagctggcc agagtaatgt tgtaacatag caccagaatc ttaccagggg
     1741 tagtttcagc caggtaaaga caacggaaca aaagaatcat ggtttttcca gagccggcta
     1801 ccccatgaat aacacgatgc ccatcaccaa tatttctggc cagaatctcc tgctgaatat
     1861 ccattatttt gatttgtgag tgctgtctga cggccatttc aggaaacaga tgagcacgca
     1921 gaatatctct aacctgtggt gtcacccggg ttctgaatcc catcgtaaac atcccggcaa
     1981 ttttttggcg gaatatatca ggtaaaacag actgagtcat atcatcctga cagattgtct
     2041 gcgctgaagg gaaaatcctt tccacagcat tttcatcatt cccagccaga gccttcagtt
     2101 gctggcgggt aatacgggta aacacaacac catacgccca ggccagattc agacgcccct
     2161 tatactgacc atcattctgt cgcaaacgtg agtctgcagg taaagcattt acggtatcac
     2221 aggcataacg acgaacctgt agcagcggat ttatttctga tttaagaata ccgttagttt
     2281 caagaatgac ctgttcgtga tttacctgat gcaacgtgtg aacggtccag tccttgactt
     2341 caagaaatac cagaccattc tccgggtcaa taatgacaaa atcaggatgt cggtttttcc
     2401 gtcccaccgg aatatcgtac cagaccagac actcattact cataaatgcc tcaagacggg
     2461 atgcaacccg cttttccccg gcagtcatcc gctcctcggt aacacctgat atatttatca
     2521 gtttagcc
//
LOCUS       DAZKFU020000406         2521 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZKFU020000406 DAZKFU020000000
VERSION     DAZKFU020000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.408"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2189)
                     /gene="ypjA"
                     /locus_tag="ACHOQQ_RS23840"
                     /old_locus_tag="pgaptmp_004768"
     CDS             complement(<1..2189)
                     /gene="ypjA"
                     /locus_tag="ACHOQQ_RS23840"
                     /old_locus_tag="pgaptmp_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HGU4472906.1"
                     /translation="MNRISRYYFHRSVLSLLIAAMIYAPPGMTAFTSNVIGVVNDETV
                     DGSQRVDERGTTNNAHIINHGNQEVYGGISNGSVIDTGGHQEVSGHGSYQGQANNTVI
                     NGGSQTISEGGISTGTIINDKGTMSVLTNAKADATRIDNGGAMDVAGNATNTIINGGT
                     QNIYNHGIATGTNINSGTQNIKSGGKADTTNISSGSKQVVEKGGTATGSNIRAGGTLI
                     VDTGGIAHGVYLDTGSALVANTGAGTDIDGYQRSSHFTITGGRAEHVVLENTGELTVV
                     AQTSAVDTIVDAGGKLIVHEEAVAYTTRLNNGGTLDVREKGSATGILQSTQGALVATT
                     RATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTTSAKTQVNTGGREIVKTKA
                     TATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTLTVNDNGKATD
                     IVQNSGAALQTSTANGIEISGTHQYGTFSIAGNLATNMLLENGGNLLVLAGTEAHDST
                     VGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQPLARAEDLQVAGGTAIVYAGTLAD
                     ASVSGATGSLSLITPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLADLTAASRGSVW
                     LNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTTNELSGSGNFY
                     LHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG"
     gene            complement(2254..2373)
                     /locus_tag="ACHOQQ_RS23845"
                     /old_locus_tag="pgaptmp_004769"
     CDS             complement(2254..2373)
                     /locus_tag="ACHOQQ_RS23845"
                     /old_locus_tag="pgaptmp_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU4472907.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2334..2519)
                     /gene="ymgJ"
                     /locus_tag="ACHOQQ_RS23850"
                     /old_locus_tag="pgaptmp_004770"
     CDS             complement(2334..2519)
                     /gene="ymgJ"
                     /locus_tag="ACHOQQ_RS23850"
                     /old_locus_tag="pgaptmp_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HGU4472908.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
BASE COUNT          557 a          688 c          581 g          695 t
ORIGIN      
        1 cctccccctg ttttcaccag cgtcatcgca tcgtcagact gtgggctgac gccggtatcc
       61 tgaacaaaga ttttaaaatt accagtggcg ttgttgttga cgaccagttg atcgccccgg
      121 gagccagcaa cgttggtatg caggtagaaa ttaccgctac cggaaagttc attggttgtc
      181 agcgtattgt agataccgtt agttgtggca ggcgctgctg tttgtgctga taaataaaca
      241 ttaccgtcgt taagtagcaa actgtttact ctatactcgc agttgctggt gcctgcacag
      301 gaattattgc tgttaagcca gacactgccc cggctggcag ccgtcaggtc ggcaagcgtg
      361 gtatcgacgc catttcccag agtcaatgtc gcgctatcgg taatccggac cgccccttca
      421 agttttactg gcgtaacatt atcccgtggc gtgattaacg acaggcttcc tgtcgcgcca
      481 ctgaccgatg catccgccag cgtacctgcg tagacgattg ccgtcccgcc agcgacctgg
      541 agatcttctg cccgggccag aggctgaaac tcatcttttg accgcccaag ggtatattgc
      601 ccgccagagt taacctttgt ggcggagtcc tgacccaggt tttgcattgc accacccttg
      661 ccaaccgttg agtcatgggc ttcggtacct gctaatacca ataaattacc accattttcc
      721 agcaacatat tggtcgctaa atttccggca atggaaaagg tgccgtactg gtgagtaccg
      781 ctgatttcaa taccgttagc cgtgctcgtc tggagagcgg caccgctgtt ctggacgata
      841 tctgtcgctt tgccattatc gttgactgtc agcgtaccgc cttcattgat cgttgttttt
      901 attgcctcac cgttagccga aactgtttgt attccgccgt cgttaatcgt tgtctcattc
      961 gccacaccct cgacgatttg ttcgccgccg gtgagcgtcg tgcctgtcgc agtggctttt
     1021 gttttgacga tctcccgtcc gcccgtattg acctgtgttt tggcggaagt ggtgtctgac
     1081 tccacggtta acacgccgcc atttgccagc aggatattgt tcgccgcacc ctgctcgatg
     1141 ctgaacgcga cgccatcagc gcgtgtccct gtgacacgcg tcgccctggt ggttgcaacc
     1201 aacgcgccct gagtactctg cagtatcccc gttgcgctgc ctttctcccg cacatcgagt
     1261 gtgccgccat tgttaagtct ggtggtatat gctacggcct cctcgtggac aatcagctta
     1321 ccgccagcat caacgatggt atctaccgcc gaggtttgag ctacgaccgt cagttcgccg
     1381 gtattttcca gcacaacatg ctcagccctg cctccggtaa tagtgaagtg agatgacctc
     1441 tggtatccgt caatatcagt ccccgcgccc gtgttggcaa ctaaagcact ccccgtgtcc
     1501 aggtaaaccc catgtgcgat accgccggta tcgacaatca gcgtgcctcc ggcgcgaata
     1561 ttgctgccag ttgccgtacc tcctttctca acaacctgct tgctcccgga agagatattt
     1621 gttgtgtcag ctttcccgcc gctcttgata ttttgcgtgc cactgttgat gttggttccc
     1681 gtagcaatac catgattata aatattctgt gtgccgccat taattatggt attagtcgcg
     1741 ttcccggcaa catccatggc cccgccgtta tctattcggg tcgcatcagc tttagcattc
     1801 gttaaaactg acattgttcc tttatcgtta attatcgtcc ctgtagaaat tcctccctca
     1861 ctaattgtct ggctgccacc gttaatgact gtattattag cctgcccctg ataagagcca
     1921 tgaccagata cctcttgatg tccaccagta tcaataacac ttccattaga aatcccgcca
     1981 tatacctcct gattgccgtg attgatgata tgagcattat ttgttgtacc tcgttcatct
     2041 actctttggc tgccgtctac agtctcatca tttacaacgc caataacatt agaagtgaag
     2101 gccgtcatcc caggcggggc atatatcatg gcagctatca ataaggaaag tactgagcgg
     2161 tgaaaataat agcgactgat tctgttcata aatttcatcc tctgaaaaat gaatactgag
     2221 tagcgtctaa gtaacctaag ctcggctgca acatcagccc gcaggcacca gaccagggga
     2281 ttcatcctga agagacagcg caggtgtatt gtgttcgccg ctcaccaaag acatcatgat
     2341 gaaatgatga tatcccgcat aagaaagagg cattttttaa acgcagtgcg ctgaagtgta
     2401 gttggataaa aaatcactct tttcagataa tactgtcgta cgcttatctt tcgacaatga
     2461 ggccgtcggc aaaataaaat gactaatgta atcgttgctg atgaatatct tctgctcaca
     2521 t
//
LOCUS       DAZKFU020000407         2508 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZKFU020000407 DAZKFU020000000
VERSION     DAZKFU020000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.409"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..407
                     /locus_tag="ACHOQQ_RS23855"
                     /old_locus_tag="pgaptmp_004771"
     CDS             84..407
                     /locus_tag="ACHOQQ_RS23855"
                     /old_locus_tag="pgaptmp_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4472909.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            413..1549
                     /locus_tag="ACHOQQ_RS23860"
                     /old_locus_tag="pgaptmp_004772"
     CDS             413..1549
                     /locus_tag="ACHOQQ_RS23860"
                     /old_locus_tag="pgaptmp_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4472910.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1546..>2508)
                     /locus_tag="ACHOQQ_RS23865"
                     /old_locus_tag="pgaptmp_004773"
     CDS             complement(1546..>2508)
                     /locus_tag="ACHOQQ_RS23865"
                     /old_locus_tag="pgaptmp_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4472911.1"
                     /translation="QLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFESKV
                     GLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGI
                     ITYLSKHVIDKIKDISDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVA
                     SFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVR
                     DYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLV
                     AIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          618 a          580 c          635 g          675 t
ORIGIN      
        1 ttatataaat aagaaaattg cactgcatca tggcatttca taatatgcgt taattgttct
       61 tttttatatt ctctgaggga aatatgttcc tggactattt tgcactggga gtgcttattt
      121 ttgtatttct ggtgatattc tatgggatta ttattttaca tgatattccc tacctgattg
      181 ctaaaaaacg taatcatcct catgccgacg ccattcatgt tgctggttgg gtgagtcttt
      241 ttacgctgca tgttatctgg ccatttttgt ggatttgggc cacgctttac cgcccggagc
      301 ggggatgggg aatgcaaaac catgactcct ccgttgtgca actgcaacag cgcattgccg
      361 ggctggagaa acagctcgcc gacatcaaat cctcttctgc cgagtaatac ttatggattt
      421 actgattatt ttgacctatg tggctttcgc atgggcaatg tttaagatct tcaaaattcc
      481 tgtaaataaa tggaccattc ccacagcggc cctggggggc atatttattg tcagcggttt
      541 aattctgtta atgaactata accatccgta tacctttaaa gcgcaaaaag cggttatttc
      601 tattcctgtt gtcccgcagg tgacaggcgt ggtgatcgaa gtgacggaca agaaaaatac
      661 gctaattaaa aaaggtgagg tgctatttcg actggacccg acgcgttatc aggcgcgggt
      721 ggatcggctg atggcggata tcgttaccgc agaacataaa cagcgggcgt tgggcgcaga
      781 gttagatgag atggcggcga atactcagca ggcaaaggcc acgcgggata aattcgctaa
      841 agagtaccag cgttacgcac gcggcagcca ggcgaaagta aacccgtttt cagaacgcga
      901 tattgatgtg gcacggcaaa attatctggc gcaggaagcc tccgtaaagt catcggcggc
      961 ggaacaaaaa cagatccaga gccagctgga tagcctggtg ttgggtgaac attctcaaat
     1021 cgccagcctg aaagcgcagc tcgcggaagc aaaatataac cttgagcaga cgatagtgcg
     1081 tgcgccgagc gatggttatg tgacccaagt gctgattcgt ccgggtacct atgccgcgtc
     1141 gctgccgcta cgtccggtga tggtgtttat acccgatcag aaacgacaaa tcgtggcgca
     1201 gttccgtcag aactccttgc tgcgcctggc tcccggcgat gatgcggaag tggtatttaa
     1261 cgctctgcca ggcaaagtgt tcagcggtaa gctggcagcc attagtccag ctgttcccgg
     1321 cggagcttat cagtcgaccg gcaccttaca gacgttaaac acagcgccgg gttcagatgg
     1381 cgttatcgcg accattgaac tgaatgagca cactgatttg agcgctttac cagacggtat
     1441 ttacgcccag gtggcggtct actctgatca tttcagccat gtctcggtga tgcgcaaagt
     1501 gttattacgc atgaccagct gggtgcatta cctttatctc gatcattaat cttcgctttc
     1561 cgccgcttgt tcaggccgca gttgctgcga ctgagcaagc tggtggcgaa tttcacgcag
     1621 catttcatcc agcacatact ggacccgcca gggctggtat tcccgtttac ggaagatagc
     1681 caccagatcc agccaccatt catacgggtg ctgaaagcag ggcaccagcg tgccgtcttg
     1741 caggtcgctc tgcacacttt tgtctggcgc aaacacaatc cccaggtgat ttcgtgccag
     1801 ctccagcgca gattgcgtgt tgtcgcaaac gtaatttccc ttcacgcgat aatcccgcac
     1861 ctctttactc ccggcgacgt tgaagcgcca gatattcgcg tcgtcgatca tcatcgagtc
     1921 gatcaaaata caggagtgat gttcgagttc gtcaggacgg ctaatgggat gtttctcaag
     1981 atagcgctgg ctggcataag cggttacggc atattgggta ataaaactgg caaccagcga
     2041 ttcatctttt ggtggggcat agctgattaa aacatcacaa tcatcaggaa attcgacgcc
     2101 ttcataaaag gcattacgct caagattgca ggtttttagc gataaggtga tatcactgat
     2161 atctttaatt ttatcgatta catgtttgga taaataggta attatgccgg ttggggcgta
     2221 gatggtgacc cgaccacgtt tctcatgctt ataatctgca ataaaattat taagctgctc
     2281 gtttctgtcc agcatgtcgt tgatgtacgg taatagcgcg gtgccaaaag gtgtgagcat
     2341 cagttgtcgg gtggttcggt caaaaacttt taaacccact tttgattcaa aatcagcaag
     2401 gtatttgctg acgttggcct gtgcgatgcc aagtacagtc gcggcatggc tgatattttc
     2461 actggcagcg attaccgaga taatttttaa ctcccggtac ttaagttg
//
LOCUS       DAZKFU020000408         2490 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZKFU020000408 DAZKFU020000000
VERSION     DAZKFU020000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.410"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            138..2417
                     /gene="ydeP"
                     /locus_tag="ACHOQQ_RS23870"
                     /old_locus_tag="pgaptmp_004774"
     CDS             138..2417
                     /gene="ydeP"
                     /locus_tag="ACHOQQ_RS23870"
                     /old_locus_tag="pgaptmp_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HGU4472912.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICSGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTQSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          651 a          588 c          636 g          615 t
ORIGIN      
        1 taacgataat agattaaaaa tagaaagaga gtagtcccct actcttatat atccatgttg
       61 gcgataatcc ctttgtatgt actgtgcatc atcgctatta caaatcctaa taattcattt
      121 ccacacagga taagtagatg aagaaaaaaa ttgaatccta ccagggagct gccggtggtt
      181 ggggtgctgt taaatccgta gcgaatgcag tacgtaagca gatggatata cgccaggatg
      241 ttattgccat gtttgacatg aataagccag agggctttga ctgtccgggt tgtgcatggc
      301 cagatcctaa gcacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa gcaatcgcct
      361 gggaagtcac ggataagcag gtaaacgcct ctttctttgc tgagaatacg gttcaatcat
      421 tacttacctg gggagaccac gagcttgagg ctgcggggcg actcactcag cctttgaaat
      481 atgatgccgt cagcgactgt tacaagccat taagctggca acaagctttc gatgaaattg
      541 gcgcacgcct tcaaagctat agtgatccca atcaggttga attctatact tcgggccgca
      601 cttccaatga ggctgccttt ctttatcagc tttttgcccg tgaatacggg agcaataact
      661 ttcccgactg ctccaacatg tgccatgaac cgacaagcgt gggtttggca gcgagtatcg
      721 gtgtaggtaa agggaccgtg ttgctggaag actttgagaa gtgcgattta gtcatttgca
      781 ttgggcataa ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc gctttagtga
      841 aacggggagc gaaaatgatc gccatcaatc ctctacagga acgtggcctg gagcgattta
      901 ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg gccagtgcct
      961 actacaacgt gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg cgcctgttaa
     1021 ttgagcgcga tgatgctgca agtgccgcag gtcggccctc attgctggat gacgaattta
     1081 ttcaaacgca taccgtcggc tttgacgagc tacgccgtga cgttctcaat tccgagtgga
     1141 aagatatcga acgtatttct ggactaagtc agacacaaat cgccgaactg gctgatgcat
     1201 atgccgctgc cgaacgcacc attatctgtt acggaatggg gatcactcag cacgaacatg
     1261 gtacccagaa tgtacagcaa ctggtcaatc tgctgttgat gaaaggtaac attggcaagc
     1321 ctggtgcggg tatctgccca ctacgtggcc actctaatgt acagggcgac cgaaccgtcg
     1381 gtatcaccga gaaaccgtct gcagagtttc tggctcgcct gggtgagcgc tatggcttca
     1441 caccacctca agcacctgga catgctgcaa ttgccagcat gcaagcaata tgttcagggc
     1501 aggctcgagc attgatctgc atggggggca actttgcact ggcaatgcca gatcgggaag
     1561 cgagcgctgt accgttaacg caattagatc tggcggtaca cgtagccact aagcttaacc
     1621 gctctcatct gctgaccgca cggcatagct atattctgcc ggttctggga cgtagcgaga
     1681 ttgacatgca aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg tcgatgattc
     1741 atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag tgtgcagtgg
     1801 tcgcgggaat cgcgcaggca gcactacccc agagcgtggt agcctgggag tatctggtgg
     1861 aagattatga tcgcattcgc aatgacattg aagctgtgct gccagagttc gccgattata
     1921 accagcgcat ccgacatcct ggtggttttc acctgataaa cgctgctgct gaaaggcgct
     1981 ggatgactca gtcaggtaag gctaatttca ttaccagcaa agggctgtta gaagatccct
     2041 cttcagcgtt taacagtaag ctggtcatgg cgacagtacg cagccacgat cagtacaaca
     2101 cgacgattta tggtatggat gatcgctatc gaggggtatt cggtcaacga gatgtggtct
     2161 ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga gttaatctta
     2221 ttgcgcttac gccagacggt aagcgcagct cacgccgcat ggatagatta aaagtggtca
     2281 tttaccctat ggctgaccgc tcactggtga cctattttcc agaatcgaat cacatgctaa
     2341 cacttgataa ccacgatcca ttaagtggca ttcctggcta taaaagtatt ccggttgaat
     2401 tagaaccatc aaattaatgc ctcttctcat ttcttctgct gtcatccgca cagcagaaga
     2461 atacctcatt gactattatc gcgcaatttg
//
LOCUS       DAZKFU020000409         2477 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZKFU020000409 DAZKFU020000000
VERSION     DAZKFU020000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.411"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(82..558)
                     /locus_tag="ACHOQQ_RS23875"
                     /old_locus_tag="pgaptmp_004775"
     CDS             complement(82..558)
                     /locus_tag="ACHOQQ_RS23875"
                     /old_locus_tag="pgaptmp_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4472913.1"
                     /translation="MQIIIMTRDRYLEYGLMCVLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRCGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYGFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
     gene            complement(621..827)
                     /locus_tag="ACHOQQ_RS23880"
                     /old_locus_tag="pgaptmp_004776"
     CDS             complement(621..827)
                     /locus_tag="ACHOQQ_RS23880"
                     /old_locus_tag="pgaptmp_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472914.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
     gene            complement(955..1935)
                     /locus_tag="ACHOQQ_RS23885"
                     /old_locus_tag="pgaptmp_004777"
     CDS             complement(955..1935)
                     /locus_tag="ACHOQQ_RS23885"
                     /old_locus_tag="pgaptmp_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4472915.1"
                     /translation="MSAYVNPEASNGVAVGSWASADALNSVALGTGAQAMHRYSVALG
                     QQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTRDTDAATVGQLNRKADE
                     VYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNTRGLLSQRGVLETHEER
                     LNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQEMATENRAAVSRNRQV
                     GERNSRAIASQARTLQAHERRMNSQQRQINENHEEMKRAAAQSAALAGLFQPYSVGKF
                     NATAAVGGYSDKQAVAVGMGYRFNEQAAAKAGTAFSDGDVSWNVGVNWEF"
BASE COUNT          660 a          732 c          510 g          575 t
ORIGIN      
        1 aactgttatc acaaataaaa tgcatacccg ttccacatga aatttatcac gcctggccac
       61 ataacattaa ttatcccgga tttaccggtc tgcggtgctg cagagatatt taatactgac
      121 gccgcgcaga ccatttttct ccatcgcatt gtaaagatgc gcacataccg tcttcatgct
      181 tatccccagc tggtctgcca gaaaggtaaa accgtactca tggaaaacaa ctccgaagac
      241 caccaccatc tcggtacggg tcagtggtct tgccgtatgt ccgaacagcc acgccccaca
      301 acggatggcc tgcctgatat ctgtcaggca tctcacagag gaaacaggaa tgacaagaaa
      361 acgacgccca cagaacatac agtatgtaag cctttccaga ttacggtcac agagaatcac
      421 ataactgtct tcgggaagtg gcagacggcg ctttccggca tcaaacagtt cacggcctgt
      481 cgtgagcctg tatccgttca gcacacacat caggccatat tccagatatc tgtcacgggt
      541 cataataata atctgcatac tctcagaccc ttatctcccg gataccggct ccgtttacca
      601 ttaccggtat aactgactca tcattcataa cggaaactgt ctgccgcata catcagtgcc
      661 cgctcttcca gctctgacag actcagctct ttcagttccc cttctccttc agcaagaaga
      721 caacattcaa aaacagctgc atcgtcgcat tccagacgaa cctgcaccct gtcagcccga
      781 ccgtttacag taaatacctg cactgaactt atgcgatagt tcagcatata cactctccct
      841 ctcagctcct caggaatacc ccggaaccgt cagtcatgcc accggggaga aaagcaggca
      901 gcgaatcatg accgcataca gaacgctgcc cgccccggtg actcagaccc gggttcagaa
      961 ctcccagttg acacccacat tccaggaaac atcaccgtca ctgaacgcag tacctgcctt
     1021 tgccgcagcc tgttcgttaa agcgatatcc catgcccacc gcaacagcct gcttgtcact
     1081 gtacccgccc actgccgctg tggcgttaaa tttacccaca ctgtatggct ggaaaagacc
     1141 ggccagcgca gctgactggg ctgctgcccg cttcatttcc tcatgattct cattaatctg
     1201 acgctgctga ctgttcatac gacgctcatg cgcctgcagg gtacgtgcct gagaagcaat
     1261 ggcgcgactg ttgcgttcac ccacctgacg gttacggctg accgcagcac ggttttctgt
     1321 ggccatttcc tgtgtacgta ccagcccttc ctgtacagta attcgttcac cggttccctc
     1381 accgcgcgtc acataaccat gactgtccac agcaacggta cttccggtat ttatctgctg
     1441 ttgctggctg ttcaggcgct cttcatgcgt ctccagcacg cctctctggg aaagcagtcc
     1501 cctggtgtta cggtttatgg ccaccgtgtt ccggataata ttctccctgt tctctgcagc
     1561 aaccgtatcg gtatggtcac gcgccttaag tgcttccgcg gcaatccgcc cactgacgtc
     1621 actgtacacc tcatccgctt tcctgttcag ttgcccgaca gtggctgcat ccgtatcccg
     1681 tgtaccatca ctgacaccac cgagaacccg ggttttatcc ggtacacgcg gactggccgg
     1741 gtttgtgaca cctgagtcat atccgatgta gacttcattt tcacgggttg taccggactg
     1801 ctgaccaaga gccactgaat acctgtgcat tgcctgtgca cccgtaccca gtgctactga
     1861 atttaaagca tctgcagatg cccagctacc tactgccaca ccattagacg cctccggatt
     1921 tacataagca gacatcccca cggatgtgga aaacggcgca tgcgcatacg atgcagagcc
     1981 aagagctgtc cctgccttcc cggcattcgc ccatgccccc actgccgtgg agtcagtctc
     2041 tgatgcagag gctgtgtccg gagacgccca ggactgccat cctgcgcaaa cgccggagca
     2101 ccggcaatca gaaaggaaat aagcgataaa gtaataccgt gtcgatttat tttcataaac
     2161 aacctcattg atatacacaa tatactggca cagagttaaa ttaatatata atcaggagag
     2221 aagtgaaaga gtacccggtg cgaaaatgaa ttaatctgag cagatttaac ggtaaacgaa
     2281 gcgcaataaa ttaaaattaa aaaacaaaat cacttgcagt ctattatctt tacgttcctc
     2341 accgcaaccg cacacaaaaa caaaacacac aatgcattga ttttaaacaa aaaaaacaaa
     2401 acagaaactc aacacataaa aatcagacaa tataaacaga attgtgattt atatcctcaa
     2461 gcgctgtatt aacgaaa
//
LOCUS       DAZKFU020000410         2463 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZKFU020000410 DAZKFU020000000
VERSION     DAZKFU020000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.412"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..847)
                     /locus_tag="ACHOQQ_RS23890"
                     /old_locus_tag="pgaptmp_004778"
     CDS             complement(<1..847)
                     /locus_tag="ACHOQQ_RS23890"
                     /old_locus_tag="pgaptmp_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HGU4472916.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLIATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRL"
     gene            complement(863..1759)
                     /locus_tag="ACHOQQ_RS23895"
                     /old_locus_tag="pgaptmp_004779"
     CDS             complement(863..1759)
                     /locus_tag="ACHOQQ_RS23895"
                     /old_locus_tag="pgaptmp_004779"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4472917.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLAGVLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMV
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(1759..2364)
                     /locus_tag="ACHOQQ_RS23900"
                     /old_locus_tag="pgaptmp_004780"
     CDS             complement(1759..2364)
                     /locus_tag="ACHOQQ_RS23900"
                     /old_locus_tag="pgaptmp_004780"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4472918.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
BASE COUNT          771 a          624 c          512 g          556 t
ORIGIN      
        1 ataaacgact aataaagctt tgcatttttt caaatgcctg cgccctgttc tcttcaccca
       61 acgtgtcgtg ataaaagcct ggcagcagat gcagctcttt cagagggcta cgtaatctct
      121 gataaaaatc aatctgcggt tggcgatgca ccacatagtc atcgcctgat atcagaagtt
      181 gcgtggggag cgtaatcgcc gccgcatcgc taacaatacg ttcagacgtt ttatagagat
      241 ctagcaagat attgacggca atcgcccttg tgatcagcgg atcattattg aaactcgcca
      301 cccgttgccg atcgtgggtc aaatagcgtc ctttcacata ggaattaata aaaaacagac
      361 cacgcagacg atgccataac gccagcgcag gacgtgccag cggcacatac aatttaacct
      421 taaaggccgg agaagccagc accagcccgc gaattgcagg cgcataatca tgaacccatg
      481 tggcaatcag cactgcgccg acgctttgcg cgatcactac cacctcttcc agtccaacct
      541 ggctgtcgct ggcagcaaaa cggacaaatt catcgacatc ccgcactgaa cgcgcaagag
      601 atgggctata accacgcggc cccgaagttt ttccatgccc tcgggcatcc catgcataaa
      661 aagcagtatc tggcatcgcc agttcatcaa cgagatgttg tagacgacca gaatgttcat
      721 gaccgcgatg aaataagacg atgacctttt tcgccccggg ctgtaaagcg ggccagtgcc
      781 gataaaacaa cgctgtatta tcactggtgg taaaaaaatg ttccccaggg atgcgtgaat
      841 tttccatttt ctctattttc cttcagtaac agcagtagcg aataaaataa aaaaataccg
      901 gagcggtgaa aattaatgag tcaatcctgt caaggagtcc gccatgtcct ggtaatagtt
      961 ttccgctatc tttaacacca atatctcgtt tgatggctga catcactaca tcgccgcaaa
     1021 aaccactaat accaattaac aaacccgcta ataatgcctg taatgtattt agcggtgtca
     1081 gcaacggccc aataatcagt gacgcaacca tggtggtgat tacgcccccc accagacctt
     1141 ccagcgtttt tcccgggctg actttgggga ccacttttct tctgccgcag gatttccccc
     1201 ataaatactg tgcaatatcg tttgactctg ttaacgccaa aagaaataac accagtaacg
     1261 ccccgccctg ggtatttgtg gttggcaaca ttaataacca ggcggcatga ctcaaggcga
     1321 aaacggtcat tatccagccc caaaaaatgg cgctcacggt atgcagaaaa ccggaggtat
     1381 cacctactaa aactcgccag gtggttaata tcagaacccc agccaaaggt ataaataaca
     1441 gaaacagctc aaagcaatta aaaccgataa gcaaatagtt aagaggaata acccaatata
     1501 accaacgcgg aaaatgtacg gatataagcg tacaatattc tttcaatgcc agaaaactta
     1561 tcagcgcaaa aaacgtcaac gtcatccagc gtggacctga aatcaccagt gaaaaacaaa
     1621 tgacgatgcc ccaccaggta cgaatacgta atgtaacttc atcccctttt ctctccggta
     1681 accgccatac ccgaaaacgg tttatcagcg ttgctaaaat taacaacgcg aaaagtgttg
     1741 ccagagattt ttccagcatt atctttcagc actcatcagg agcacactcc gacaacgatt
     1801 aatcgcagtc cagagaagca gaattgaagc aatgctccat aaaagattat tccattgcat
     1861 ccactgtgga taaatggcaa cggccagccc ccacaaccca aatattaacg cgcgatcgct
     1921 tttgccaaaa ggaccgacat aactgcgaac accgttaatc gtctgcgcga gtaaaccgca
     1981 aaactcagtc aatatggtgc aaaataacat gagtattacg agtgatgcgt tactttctgg
     2041 taaaaataaa aacggtaaat agagcgcaat atcggaaata acatcgccag tttcattcaa
     2101 aatcgccccc agacgtgttt gctgattgca ctcacgcgcc aacatgccat ccagcgcatt
     2161 gagtgccata cggataaaaa gcacgatggg caatagcaaa aagaggatgg gttgtgcgac
     2221 caacatcaac agcaatccgg taataagaga aagcgccagt gcagcaagag tgatgtgatt
     2281 cgctgtaaca tggtgcttat aaagccaaaa catcgtcggc cttaacagcg actgaaagag
     2341 cggttttatc tggtagagcg tcattatcaa tccttgataa ttcggtttgc gctatggtgc
     2401 atcaattccc ctgacggaga tatcgcaaca cgacgcttca ttaaaaattc agaatatatc
     2461 aag
//
LOCUS       DAZKFU020000411         2459 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZKFU020000411 DAZKFU020000000
VERSION     DAZKFU020000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.413"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..245
                     /gene="ymgI"
                     /locus_tag="ACHOQQ_RS23905"
                     /old_locus_tag="pgaptmp_004781"
     CDS             72..245
                     /gene="ymgI"
                     /locus_tag="ACHOQQ_RS23905"
                     /old_locus_tag="pgaptmp_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HGU4472919.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            247..591
                     /locus_tag="ACHOQQ_RS23910"
                     /old_locus_tag="pgaptmp_004782"
     CDS             247..591
                     /locus_tag="ACHOQQ_RS23910"
                     /old_locus_tag="pgaptmp_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HGU4472920.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            595..930
                     /locus_tag="ACHOQQ_RS23915"
                     /old_locus_tag="pgaptmp_004783"
     CDS             595..930
                     /locus_tag="ACHOQQ_RS23915"
                     /old_locus_tag="pgaptmp_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HGU4472921.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(986..>2459)
                     /locus_tag="ACHOQQ_RS23920"
                     /old_locus_tag="pgaptmp_004784"
     CDS             complement(986..>2459)
                     /locus_tag="ACHOQQ_RS23920"
                     /old_locus_tag="pgaptmp_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4472922.1"
                     /translation="SDNSTWDIKADSTVSKLTVDNSTVYISRADGKDFEPTRLTITEN
                     YVGNNGVLHLRTELGDDNSATDKVVINGNTSGTTRVNVTNAGGSGAYTLNGIEIISVE
                     GESNGEFIKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVA
                     PTPVLRPEAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQVRSSRLWLRQIGGHN
                     AWRDSNGQLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYR
                     SKGSVRGYSAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATV
                     SLEAGYGFALNKSFGLEAAKYTWIFQQQAQAIWMGVDHNAHTEANGSRIENDANNNIQ
                     TRLGFRTFIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGARNLGEI
                     KLGVNGNLNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT          635 a          593 c          563 g          668 t
ORIGIN      
        1 cagattatct tacctttaca acattgcgtt aacagtcaat gacacttctt gaataaaaat
       61 attgtcaatc aatgagttat tcctcattta agattattct cattcacgta aaaaacataa
      121 ttcctatcat cacagccacg ctcattttga attatctgaa taatagcgag cgttctttag
      181 ttaagcagat cttaattgaa gacgaaatta ttgtgtgtgc aacttactta ataccggaca
      241 tctaagttga agaaaaaaat acttgccttc gggcttatct cagcattgtt ctgctccacg
      301 ccagcgatgg cggatatgaa ccgcaccacg aaaggtgcat tgctaggcgc aggcgttggt
      361 ttacttactg gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg cgctggtgtt
      421 ggtgcagtaa cagaaaaagg ccgcgacggt aaaaatgcgc gtaaaggtgc aaaagtgggt
      481 gccgccgttg gtgctgtcac tggcgttctg accggcaatg gcctcgaagg ggcgattaaa
      541 ggtgccgtga ttggcggtac gggtggtgca attctgggta aaatgaaata aggaatgatg
      601 atgaaaaaat ttgctttact ggcgggtctg tttgttttcg caccaatgac ttgggcgcag
      661 gactacaata ttaaaaacgg tttgccgtca gaaacctata ttacttgcgc agaagccaac
      721 gaaatggcaa aaactgacag cgcacaggtt gcagaaattg ttgcggtaat gggtaatgcc
      781 agcgttgcca gccgtgattt aaaaattgag caatctccgg aactgagcgc caaagtggtc
      841 gaaaaactga atcaggtttg cgctaaagac ccgcaaatgc tgttgatcac ggcaattgat
      901 gacaccatgc gtgctatcgg caaaaaataa tatttaatat gttaccgcgt cgatgaattc
      961 gacgcggtat tttagctaac ggggatcaga acttatattt caggcccacc atcactgcgg
     1021 tgtcattgta gccattatca cccagctgca cgcccacatt accccagacg ctggccgccg
     1081 gattcagatt gccatttacg ccaagtttaa tttcccccag attacgcgcc ccgtcttgtt
     1141 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     1201 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     1261 aggtgcggaa accaagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     1321 cattggcttc cgtatgcgca ttatgatcga cgcccatcca gatagcctgt gcctgttgct
     1381 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaagccgt
     1441 aacccgcttc cagcgagacg gttgcgcctt tcgcgctata ggattcatag gctaattcat
     1501 cccctttcac cgagttttta aaccagctat attgcgccca ggcgtcaatg tatgcgcctt
     1561 ttttactaat gtcatcggca aaccaggtgg catacagccc tgcgctatag cctctgacac
     1621 tccctttcga acgataatcc gacacgctgg aatgagttga gttgtagtcg cgggcataac
     1681 cagccatcac tcccaaacgc caactgtcac tgtcggtaaa accaccggtt aacagatcgg
     1741 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     1801 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgcacc tgttcagtta
     1861 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aacagagtgt
     1921 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg ggggtgggcg
     1981 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     2041 tagtcagata ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt
     2101 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     2161 tgataatctc tatcccattt aacgtataag ccccgctgcc gccagcattg gtaacgttaa
     2221 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     2281 cacccaattc agttctgaga tgcaatacgc cattattacc aacgtaattt tcagttattg
     2341 ttaatcttgt tggttcgaaa tcctttccat ctgcccggga aatatagacc gtactattgt
     2401 caaccgtgag cttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaa
//
LOCUS       DAZKFU020000412         2454 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZKFU020000412 DAZKFU020000000
VERSION     DAZKFU020000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.414"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1077
                     /locus_tag="ACHOQQ_RS23925"
                     /old_locus_tag="pgaptmp_004785"
     CDS             <1..1077
                     /locus_tag="ACHOQQ_RS23925"
                     /old_locus_tag="pgaptmp_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4472923.1"
                     /translation="SALSTDMIVPALPELQKAFHADYSLIQLSISGFLIVFAFSQLIS
                     GTIGDYFGKVRVMTICLIIFLIGSIVCFMANDLNMLLLGRILQAIGAGAGPVISKAIA
                     KESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVIFIVMGAFSILTILLS
                     PKETNKQEKKTHQDNVKFVTKDFICGTILVSLMLSSLFCYISISPALFMSDYGLNTFH
                     YSVVFSLSVLFFIIGNQLSKIEKISNPWVLFPLNAISVTPFLILNDAFIICVIGAMIF
                     NLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGTISFLSVKISDNGVFLGTT
                     LSISCLILALLSMFVTIIIGNNKK"
     gene            1074..1490
                     /locus_tag="ACHOQQ_RS23930"
                     /old_locus_tag="pgaptmp_004786"
     CDS             1074..1490
                     /locus_tag="ACHOQQ_RS23930"
                     /old_locus_tag="pgaptmp_004786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472924.1"
                     /translation="MNWQKKKIERSIKYQTRVFNEHINELVITRRNGKVITVNDGREM
                     IEFVSCSYLGLETHENLKHAIANAIEKFGVQLSVARTRVKVDLFEQLEKRLNKIMNGA
                     HTLTFNAVTPCHLAVLPLLASGTLPNYKFNKNHILF"
     gene            1511..2110
                     /locus_tag="ACHOQQ_RS23935"
                     /old_locus_tag="pgaptmp_004787"
     CDS             1511..2110
                     /locus_tag="ACHOQQ_RS23935"
                     /old_locus_tag="pgaptmp_004787"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4472925.1"
                     /translation="MQINRGLLQQFGETIRVDFQHKNDIEEAFKYASINNMVPISISD
                     SVGSMGGVAPVEFIVRMANRFDGYAYIDDAHGMSIYGKNGSGYVMSCLDNKLDDRIII
                     AGSLSKAFGSHGGFIACNYPDTINFIKTYGTTYAFGGPPSLPGIAACIAAADLHLDGT
                     VSLRQNDLQQVIKYFDDKFNETEVINKGTTMPIRGEPAP"
     gene            2186..>2454
                     /locus_tag="ACHOQQ_RS23940"
                     /old_locus_tag="pgaptmp_004788"
     CDS             2186..>2454
                     /locus_tag="ACHOQQ_RS23940"
                     /old_locus_tag="pgaptmp_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4472926.1"
                     /translation="MTGILLGQELRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQP
                     SLLFKWKKQYLEGSLTAVAAGEDVVPASELAAAIKQIKELQRLLG"
BASE COUNT          809 a          429 c          465 g          751 t
ORIGIN      
        1 tcggcattat caactgacat gattgtgcct gcgttaccag aactacagaa agcatttcat
       61 gctgactact cattaattca actaagtatt agtggatttc ttattgtatt tgcatttagt
      121 caactgatat caggaaccat aggtgattat ttcggaaaag tacgcgtaat gactatatgc
      181 ttaattatat ttcttattgg cagtattgta tgttttatgg ccaatgattt aaatatgctt
      241 cttttgggta gaattctaca ggcaataggt gcaggtgccg gacctgtaat atcaaaagcc
      301 attgctaaag agtcttttcc accactaaag ctaaaaagag cactttctga catatcatca
      361 acaagtgcga taataccatt aatagcacct ctggccggag cattaatact tgattattat
      421 aactggaatg taatatttat tgtaatgggt gcatttagca ttctaaccat actcctgtca
      481 cctaaagaga ctaataaaca ggagaaaaaa acacatcaag ataatgtaaa atttgtgact
      541 aaagatttca tctgcgggac aatactcgtt tcactaatgc taagctcact attttgctac
      601 atatcaatat cacctgcact atttatgagt gattatggat taaatacatt tcactactct
      661 gttgtttttt ccctgtcagt tttgtttttc atcattggaa accaattgtc aaaaatcgaa
      721 aaaatatcaa acccatgggt cttatttccc ctaaatgcaa tatcagttac accttttctt
      781 atattaaatg atgcttttat aatatgtgtt attggagcaa tgattttcaa tttaactctt
      841 ggcgcatact atccaatagc taatttcatc tcattgcaga taaacggttc acgaactggt
      901 ttagcagcat caattacagg ctttactcaa acagtaagcg cagggacaat cagtttcctt
      961 tcagtaaaaa taagcgataa tggagttttt ttgggaacca ctttaagtat tagttgtctt
     1021 attcttgcat tattgtcaat gttcgtaact attattatag ggaataataa aaaatgaatt
     1081 ggcaaaagaa aaagatagaa cgttcaatca aataccaaac aagagtattt aacgaacata
     1141 taaatgaact agtgatcacc agaagaaatg gtaaggtaat cactgttaat gatgggaggg
     1201 agatgattga gtttgtgtca tgctcttatc ttggactgga aacacatgaa aatctaaaac
     1261 atgcaatcgc taatgcaatt gaaaaatttg gagtccaact ttcggtcgcc agaacgcgcg
     1321 taaaagtaga tttattcgag caattggaaa agagattaaa taaaataatg aatggggcgc
     1381 acacattaac atttaatgcg gttactccat gccatttagc tgtactccca cttcttgcat
     1441 caggaacact acctaattac aagtttaata aaaaccatat tttattttag agaaaacagc
     1501 gcatgcaaca ttgcaaataa atagaggact attacaacag ttcggtgaaa caatcagggt
     1561 tgactttcaa cataaaaatg atatagaaga agcgtttaaa tatgcttcaa taaataatat
     1621 ggtaccaata tctatatctg atagtgtcgg ttcgatgggt ggggtggctc ctgtcgaatt
     1681 tattgttaga atggctaaca gatttgatgg gtatgcttac attgatgatg ctcatggcat
     1741 gtcaatatat gggaaaaatg gctccggata tgtaatgtct tgtctggata acaaacttga
     1801 tgacagaatt attatcgctg gttcattatc taaagcgttt ggctctcatg gtggatttat
     1861 tgcatgcaat tatcctgaca ccataaactt tataaaaacg tatggaacta cctatgcttt
     1921 tggtggtcca ccatcattac caggaattgc tgcatgtatc gcagcagcag atttacacct
     1981 cgatggcaca gtatccttgc gtcaaaatga cttacaacaa gttataaaat actttgatga
     2041 caaatttaat gaaaccgaag tcataaacaa agggactaca atgccaatac gtggtgaacc
     2101 ggccccttga aacgccagac agtggatgta tcttaaaata agagataggc ttggatatat
     2161 gtctaacact agtgctaatt ttgagatgac tggaattttg ctggggcagg agcttcgtaa
     2221 acgtaaaact cctcaggaaa aaatcgccat tattcagcag acaatggaac ccggtatgac
     2281 ggtgtcccat gtggcgcgtt tgcatggtat tcagcccagc ctgttgttta agtggaaaaa
     2341 acaatatctg gaaggaagtc tcactgctgt tgctgccgga gaggatgtcg ttcctgcctc
     2401 agaactggct gccgctataa aacaaataaa agagttacag cgcttgctgg gtaa
//
LOCUS       DAZKFU020000413         2446 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZKFU020000413 DAZKFU020000000
VERSION     DAZKFU020000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.415"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..253
                     /locus_tag="ACHOQQ_RS23945"
                     /old_locus_tag="pgaptmp_004789"
     CDS             <1..253
                     /locus_tag="ACHOQQ_RS23945"
                     /old_locus_tag="pgaptmp_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HGU4472927.1"
                     /translation="YLQTLINDVAEIKNITHVIDSEVKSLAESIRQEISTFSGMNRIY
                     LTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            253..648
                     /locus_tag="ACHOQQ_RS23950"
                     /old_locus_tag="pgaptmp_004790"
     CDS             253..648
                     /locus_tag="ACHOQQ_RS23950"
                     /old_locus_tag="pgaptmp_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU4472928.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDSLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDINVLKNIIE
                     ASSENKSESIASKDDFEEQTRRNLSMLKK"
     gene            complement(763..1095)
                     /gene="pemK-mt"
                     /locus_tag="ACHOQQ_RS23955"
                     /old_locus_tag="pgaptmp_004791"
     CDS             complement(763..1095)
                     /gene="pemK-mt"
                     /locus_tag="ACHOQQ_RS23955"
                     /old_locus_tag="pgaptmp_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HGU4472929.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(1097..1354)
                     /gene="pemI"
                     /locus_tag="ACHOQQ_RS23960"
                     /old_locus_tag="pgaptmp_004792"
     CDS             complement(1097..1354)
                     /gene="pemI"
                     /locus_tag="ACHOQQ_RS23960"
                     /old_locus_tag="pgaptmp_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HGU4472930.1"
                     /translation="MHTTRLKKVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPHRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(1447..2100)
                     /locus_tag="ACHOQQ_RS23965"
                     /old_locus_tag="pgaptmp_004793"
     CDS             complement(1447..2100)
                     /locus_tag="ACHOQQ_RS23965"
                     /old_locus_tag="pgaptmp_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGU4472931.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGPGKNYLF
                     FSHGAVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYIGVFLLGKVLAL
                     PAEPFMTKLFADKSIPDVSLTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYKNLLTLAELFLVGGITSAARIRSGGLLLPVLLHMEATTLGLLFG"
BASE COUNT          725 a          650 c          507 g          564 t
ORIGIN      
        1 atatctccag acattaataa atgatgtagc tgaaataaaa aatattactc atgtaataga
       61 cagtgaggtg aaatctctgg cagaaagcat caggcaggaa atttcaacat tctcaggaat
      121 gaaccgaatc tacctcacag gcgggggagc tgagcttatt taccctcaca taaagcaata
      181 tttcccgaat ctgaaagtga acaaggtaga tgaacctcag tttgcattgg tgaaagcaat
      241 ggtgcacgcg taatggaaag cagtgatccg aaaaaaagaa agaaagttgt ggcatacctg
      301 cacccgacac tttacccgca ggacagcctg acacaacaaa caatagactc tctcccgata
      361 caaatgcgag gagacttcta cagacaatct cttatttgcg gagcagctct ttattctgtt
      421 gacccccgac tgttaacgct aatcagtggt tttttcagtg agaaaatcac tgcagaaaat
      481 ctggtaaaac ttatagagca aacaacagga tacacatcca catcaattga tataaatgtg
      541 ttaaaaaaca ttatagaggc atcttcagaa aacaaatcag agagcatcgc ttcaaaagat
      601 gatttcgaag aacaaaccag acgcaatctg tctatgttaa aaaaataata aaagaagagc
      661 aggttcagtt attacctgct cttcttttat aatgagggac cagcagcggt tttagtgccc
      721 taaatcgaac gttttcgtcc ggttgcccct caaaccccat gttcaagtta gaatagtgga
      781 cagacggcca agaacttcgt tcatgatagt ctctggaacc cgttcgagtc gttttccgcc
      841 ccgggctttc atatcaattg tccggggttg atcgcaacgc acaacacctg tggtacgtat
      901 gccaacacca tccagcgaca ccgcaaagcc ggcagtgcgg gcaaaattgc ctccgctggt
      961 tacgggcaca acaacaggca gtcgggtcac gcgattaaag gccgccggtg tgacaatcag
     1021 caccggccgc gttccctgct gctcatgacc tgcggtagga tcaagcgaga caagccagat
     1081 ttcccctctt tccatgtcag atttcctcct gaccagtcgc cggtgcatcc agccattctc
     1141 gttcttcagc tgatatttca gcattcggat cacactgtgc cagtagctca gccagtgaat
     1201 attgcgggcg tctgtgcggc tcaacaatca gccggccatt atcaatgacc atgccaactt
     1261 cattatctgt acccagagac agcgcattca gcagtgccgg tggaacggtc agcataactg
     1321 agccgccaac cttcttcagt cgggtggtat gcattcttca cctccataaa agttatattt
     1381 aaatataaca tccactaaaa aaacacacca ggctttaacg cgcaatgttt aataaaaata
     1441 taactttcaa ccaaacagta aacccagcgt ggttgcttcc atatgcagca ataccggcag
     1501 cagcaggcca ccacttctga tcctggccgc tgatgtaatc ccccccacca ggaacagctc
     1561 tgccagtgtc agcaggttct tatactggct atgcgcggcg acgaacaaca acgacgttat
     1621 cagcgccccc agccacatcg tccagcagta ccgtgaacgg aagacgttca gcataatccc
     1681 ccggaacagc gtttcctcat tcaacggggc aaggataaag atggtcagca acgtcaggct
     1741 cacgtcaggt atagacttat cggcaaaaag tttcgtcata aatggctcag caggcagagc
     1801 cagcacctta ccgagcagaa atacaccgat atacaccacg gccatcgcac cgaccagcca
     1861 cggtacgcca acgttgcgca gctgaccaac gaccggtagc ggagctatcc aacggcggta
     1921 taccaggaaa acacacagca ggtacatcag aacagcacca tgactgaaga acaaatagtt
     1981 ttttcctggc ccataaagca gaacggcctg ctccatgaca aatctggctc cccagctaat
     2041 gccccatgca gccagcataa ccagcataaa ctgcagatat tgattacgtg tctgaatcat
     2101 tgtatctcct gtaaattttt aacttgtcct atttttgtca ttactacata tatacacatg
     2161 tataacaatt cagatatcgt caccaggata tgccgcgcca gcggcatgga aggcggcact
     2221 ccgctgtttc atatgatacc gccgaagccc gatgtaagcc gctacagtcg tccgaaagtc
     2281 accagcctcc tccccctgcc gtcatccgtg catcagctat gcactgagta tgcctgccct
     2341 tccctagaga atcctgccag gcttgccaca ctgatatatc ttgactttat gtaaacgata
     2401 tgacacctta acatgataat gattaccatt ctcttttaat atacag
//
LOCUS       DAZKFU020000414         2446 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZKFU020000414 DAZKFU020000000
VERSION     DAZKFU020000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.416"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            291..434
                     /locus_tag="ACHOQQ_RS23970"
                     /old_locus_tag="pgaptmp_004794"
     CDS             291..434
                     /locus_tag="ACHOQQ_RS23970"
                     /old_locus_tag="pgaptmp_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGU4472932.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            447..656
                     /gene="cspE"
                     /locus_tag="ACHOQQ_RS23975"
                     /old_locus_tag="pgaptmp_004795"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             447..656
                     /gene="cspE"
                     /locus_tag="ACHOQQ_RS23975"
                     /old_locus_tag="pgaptmp_004795"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4472933.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            822..1631
                     /gene="rlmA"
                     /locus_tag="ACHOQQ_RS23980"
                     /old_locus_tag="pgaptmp_004796"
     CDS             822..1631
                     /gene="rlmA"
                     /locus_tag="ACHOQQ_RS23980"
                     /old_locus_tag="pgaptmp_004796"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGU4472934.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1628..2194)
                     /gene="mntP"
                     /locus_tag="ACHOQQ_RS23985"
                     /old_locus_tag="pgaptmp_004797"
     CDS             complement(1628..2194)
                     /gene="mntP"
                     /locus_tag="ACHOQQ_RS23985"
                     /old_locus_tag="pgaptmp_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGU4472935.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2191..2313)
                     /locus_tag="ACHOQQ_RS23990"
                     /old_locus_tag="pgaptmp_004798"
     CDS             complement(2191..2313)
                     /locus_tag="ACHOQQ_RS23990"
                     /old_locus_tag="pgaptmp_004798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472936.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     regulatory      complement(2326..2416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT          643 a          593 c          600 g          610 t
ORIGIN      
        1 tagcagaaaa tgtgatattg cacgcactat gtaataactt ctcccactgg cctggaacaa
       61 ctgaacttat tgaactatgt tagaaactac gccagtttaa gtatctgcct gaactggcaa
      121 ggttaagcac aatgatatat cggcgcgtat tccgttgcat aagtgtgcaa aaaaagtgga
      181 agacgtatcg agatttgtgc gtctgatcga gacatgttta aaaatggctt gccataatta
      241 acgttgtatg tgataacaga tttcgggtta aacgaggtac agttctgttt atgtgtggca
      301 ttttcagtaa agaagtcctg agtaaacacg ttgacgttga ataccgcttc tctgccgagc
      361 cttatattgg tgcctcatgc agtaatgtgt cagttttatc tatgttatgc ctgcgggcga
      421 agaaaacaat ctaaggaatt tttcaaatgg caaagattaa aggtcaggtt aagtggttca
      481 acgagtctaa aggttttggc ttcattactc ctgctgatgg cagcaaagac gtgttcgtac
      541 acttctccgc tatccagggt aatggcttca aaactctggc tgaaggtcag aacgttgagt
      601 tcgaaattca ggacggccag aaaggtccgg cagctgttaa cgtaacagct atctgatcga
      661 atccactgat ctgaagtgtg aatacgcttc aatctcgcta taaagcctcg tcgaatgcga
      721 ggctttttac tatgctttat cttcgctcct ggcgttcgga tatttgcccg ccgcgtgatt
      781 cgcgttacac ttgcggcctt tagtatcctg ccggagttgt catgtcgttt tcctgtccac
      841 tttgccatca gcctcttacg cgtgaaaaaa acagctatat ctgtccccaa cgacatcagt
      901 ttgatatggc gaaagaaggg tatgtcaatc tgctgcccgt tcagcataaa cggtctcgtg
      961 atccgggcga cagcgcggaa atgatgcaag cacgccgcgc attcttagat gccggacatt
     1021 atcagccgct gcgtgatgca attgtcgccc aactgcggga acggcttgat gataaggcca
     1081 cggcggtgct ggatattggc tgtggtgaag ggtattacac acacgcattt gccgatgcgt
     1141 tgcccgaaat caccaccttt ggcctggatg tctcgaaggt agcgataaaa gcggcggcga
     1201 aacgctatcc gcaggtcact ttttgtgtcg cttccagcca ccgtttgccg ttttccgata
     1261 ccagtatgga cgccataata cgtatttacg cgccgtgtaa agcagaagaa ttagcgcgag
     1321 tagtgaagcc cggcggttgg gtcattactg ccacgccggg accgcgacat ttgatggagc
     1381 tgaaggggct gatttacaat gaagtacatc ttcatgcacc tcatgcagaa caactggaag
     1441 gttttacatt acagcagagt gatgagttgt gttatccgat gcgtcttcgc ggtgatgaag
     1501 ccgtcgcatt attgcagatg acgccgtttg cctggcgtgc gaagccagaa gtctggcaaa
     1561 cgctggcagc aaaagaagtg ttcgactgcc agacggactt taatattcac ctctggcagc
     1621 gttcttatta accgtggaag tgcgtccaga ggatctggac gccgatgccg atcagcacca
     1681 gcccgccgag aatttccgct tttttcccaa taattgagcc gataaagcga ccaaccatca
     1741 tccctaatgt cgacataatc aaggttgcac aaccaatggc caatgcggtc gcgataatgt
     1801 tgacctgaag gaaagcaaga ccaacaccca cagccatggc atccaggctg gtggcaatcg
     1861 cggtggttac cagtagccag aaaccgtgcc gacggcgcgg ctcttcatct tcatcatctg
     1921 cgccacgaaa accctcaata atcattcgcc cgccgaggaa tatcagcagc acaaacgcaa
     1981 tccagtgatt ccattcaagg acaaaccggc tggctaacat gcccattccc cagccgatca
     2041 gcggcgtcag ggtttcgacg gcaccaaaaa taaggccggt tcgcaatgct tcagaaaatt
     2101 tcggtttatg gagggtggca cctttaccga ttgatgcagc aaatgcatcc atcgacatac
     2161 caaacgcaag aagaacagta gcagtgatat tcatgacaac gtcctgaccg gggtatccat
     2221 aaaacacatc atcgccccca gcaaacatgc atacatgaac aacaagccgc gatgggtttt
     2281 cgcgttcatg caggcatagc gattgatgtg catatggtct cgcctgactg aaagtcttca
     2341 gtccgtacgt gccacgtttt gcaacgagta tgttgacacg tacatttccg gaatctggaa
     2401 atcggctact ccccaaatga gggcgcaacc ttaacatatt ttgaga
//
LOCUS       DAZKFU020000415         2442 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZKFU020000415 DAZKFU020000000
VERSION     DAZKFU020000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.417"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1227)
                     /locus_tag="ACHOQQ_RS23995"
                     /old_locus_tag="pgaptmp_004799"
     CDS             complement(<1..1227)
                     /locus_tag="ACHOQQ_RS23995"
                     /old_locus_tag="pgaptmp_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGU4472937.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDGEVMLTCHFRSGKKYTRP
                     LSVCQITPTVILRNVFLERKGNAVTSRAERVIIYGDKYAAVYYRESERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVADERVFYARGNNKNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLMAVERAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQRLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANMIDDVLQQVKKHLNAINQVASLKAEINELSVEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKSIREKQISLNEYE"
     gene            complement(1901..>2442)
                     /locus_tag="ACHOQQ_RS24000"
                     /old_locus_tag="pgaptmp_004800"
     CDS             complement(1901..>2442)
                     /locus_tag="ACHOQQ_RS24000"
                     /old_locus_tag="pgaptmp_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HGU4472938.1"
                     /translation="LSADQMAQLSRTPSLLNHTSEWITLSGQQITRLTELPPAYNLQR
                     SAQLLQQLRGLFPGNPHVQEMVDNWHKSVRSRALPEEAMTGWNEGMARLQQLAERLNR
                     LDEQRGKYMTVSELKTEVFGIMQAFNRHIPAEEQLRRYGEARNQNGSEQQQKQAELAL
                     NQLINRYQMEHTGNQERQP"
BASE COUNT          627 a          591 c          484 g          740 t
ORIGIN      
        1 ctcatattca ttcaaagaaa tttgtttttc ccggatggat ttttcataat aagaaagctg
       61 aagagaagta aatagtgcca gccttctctc cggaatcacc aatggtttga ctttcagtat
      121 cctgaagttt aacaacaaat caattctgtt tctgaatcca atccgcctgt cagaaaggta
      181 atgaatataa gccatcaggt cagttgtttt tcgtgaggaa agtcggtatt tgtgagaaaa
      241 aagctcctcc ggtcgaagat tctgactctg ccattgctgc aggtatttat actcaacact
      301 cagttcatta atttctgctt ttaacgaagc aacctggttg atagcattca ggtgtttttt
      361 cacttgttga agtacatcat caatcatatt tgcagacggt gagtcaggta agacttcctc
      421 agaacgcgaa atcgacgttg aaaaaaactg ttgtctgttc tccgtacttc ccagtcgtgc
      481 caccacatat cccagtcgtt gtttgtccat tttttcataa acattactga cagctgagtt
      541 attattggac aaaattgcca ctgttttatt ctgaattaat atattggcaa caatattgag
      601 aatagtctgt gttttacctg tcccaggtgg tccttcaata actgaaatct gtgaggaaaa
      661 tgcccgttca accgccatta actgactctc attcaggcca aacggaaaaa tcaaccccca
      721 tggcgaatca cgttttttac tctgtcctga acagtatgca tgcaaggcgg tatccggatg
      781 aggaactatt ttctttatct gtctgaggat attctcatcg atatttttat tattacctcg
      841 agcataaaaa acacgttcgt cagccaccct gcacaagtaa ttaaacacgg catgctctgt
      901 aaatgctgaa cactgttgaa agtcaagtcc ggtcgttttc atgatgtagg gtcgttcgct
      961 ctcacggtaa taaacagcag catatttatc accatagata ataacccgtt cagcccggga
     1021 tgttacagcg ttccctttcc tttcgaggaa aacatttcgc aaaattacag ttggcgttat
     1081 ctgacataca gaaagcggac gagtatattt tttcccgctg cgaaaatgac aggtcagcat
     1141 aacctctccg tcatcatttc cagcccacag agtccagtcg gtaattttat gagtcttatt
     1201 ttcaccgtca acacaaatcg tgaccatatt ctcactcagt aattatcatt taacttacgg
     1261 aaaattgtta tgaaactccc gatatttacc cggtaaaata cttgtcattg tttgaagccc
     1321 tccctaaaaa atgaccaggc atttgataac ccggtgtatt tctcccgata tgagctccgg
     1381 aggattttcc acaactccgg gtgtgttaac atattcccgc cggccatata tacattgagt
     1441 tcagtcataa cagtcaatac caagattaca ggaacgcgat gctgatacgc tggttattga
     1501 atactggtag cccctaacta ctaattcatc acgatactct gtacttactg ttttttacaa
     1561 ccattccccc aggtaaaaga gtccatttat catgcccctc tttaacgcct gccagcataa
     1621 tttaagaatg gataccgtgg gtggaattaa caatacctgc tcaggccaat gccatgtaac
     1681 cgcctcactg aaaaagccag atcatctaac caggtacaga ccaacatcgc aaaaattacc
     1741 atgtagcacg aactgctgtc atatttctgt tcaggtatgc cgctatttcg cgggttttct
     1801 tcacgccata atgcgtttca aggtagcgaa catcttcgat tgtccatggt gtgcgcgctc
     1861 ttccgggggt attttccatt tccatgtttt atctcagcca ttatggctgc ctctcctgat
     1921 tgcctgtgtg ttccatctga taacggttaa tcaactgatt cagcgccagt tctgcctgtt
     1981 tttgctgctg ttcactgcca ttctggttgc gggcttcacc ataacgacgt aactgctctt
     2041 ctgccgggat atgccggtta aaagcctgca tgatgccaaa cacctctgtt ttcagttcac
     2101 tgaccgtcat gtattttccc cgctgttcat ccagacggtt caggcgctca gccagttgct
     2161 gtaaacgggc catgccttcg ttccagcccg tcatcgcctc ttccgggagc gctcggctcc
     2221 ttacgctctt atgccagtta tccaccattt cctgaacatg tggattgccg gggaaaagtc
     2281 ccctcagttg ctgcagcagc tgcgcactgc gttgcaggtt gtatgccggc ggtagttctg
     2341 tcagtcgcgt tatctgctga ccggaaaggg ttatccactc tgacgtatga tttaacagtg
     2401 agggtgttct gctgagttgc gccatctggt ctgctgaaag ca
//
LOCUS       DAZKFU020000416         2424 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZKFU020000416 DAZKFU020000000
VERSION     DAZKFU020000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.418"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(23..1231)
                     /locus_tag="ACHOQQ_RS24005"
                     /old_locus_tag="pgaptmp_004801"
     CDS             complement(23..1231)
                     /locus_tag="ACHOQQ_RS24005"
                     /old_locus_tag="pgaptmp_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4472939.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1239..1666
                     /gene="tnpA"
                     /locus_tag="ACHOQQ_RS24010"
                     /old_locus_tag="pgaptmp_004802"
                     /pseudo
     CDS             1239..1666
                     /gene="tnpA"
                     /locus_tag="ACHOQQ_RS24010"
                     /old_locus_tag="pgaptmp_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
     gene            complement(1759..>2424)
                     /locus_tag="ACHOQQ_RS24015"
                     /old_locus_tag="pgaptmp_004803"
     CDS             complement(1759..>2424)
                     /locus_tag="ACHOQQ_RS24015"
                     /old_locus_tag="pgaptmp_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGU4472940.1"
                     /translation="RTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKETT
                     SATSKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKE
                     AIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMD
                     TRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDV
                     NKK"
BASE COUNT          574 a          607 c          576 g          667 t
ORIGIN      
        1 aggacgtgga tccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
       61 tcaacgggcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
      121 aaatgttacg agcgccattt acatcagcgt tcgctgtata tccacatgcc tggcatctga
      181 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacgcg caacgctggc
      241 ttgtgtacgc tggcggaaca gcaagcacct gaccgccacg ccagagctgc ttgtactcaa
      301 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
      361 cccggacatt gcgccccggc tggctgaccg tacccgctgc tgactttgac atgttgctga
      421 ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt
      481 gaaggtagtc cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct
      541 gcttctgcca gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca
      601 gcgtcttctg gtttttctga aaactgttta caggcccaaa gactgtgcca tctgacagcg
      661 tggcgagttt agccacgcca gcatccagtc cgaccattaa tgctgaaggg tgaaccggag
      721 ttgatacttc attttcagtc tgaatactga tgtaccactt accgcaggac tggctgacag
      781 tgacattttt cacaacaccc gtgacctgac ggctattccg gtagcgcatc cagccaagtt
      841 tcggcagaaa aatacgactg ttttcctgat cgagcttaac gccctgcggg tagcggaatg
      901 catcattctg tccccgcttt ttgaatcggg gaaaagcagc ccgcttccgg aagaagtttt
      961 tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg ctgtgacggg gcatctttaa
     1021 gccattgcgt ttcagtggca tttttccact caaccagcca ggaagccatt ttcccgtaag
     1081 ggatgtattt attaccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa
     1141 cgaaacgaca tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca ccgggtctta
     1201 actggaattt aaatgcctgt agtcgtttca caggccaaat gatactttgg tctgtgaaaa
     1261 aagaaaccga tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct
     1321 ttgtcaccag ataccgacgc cagatttttg accatgacgc gacagaaaaa ctacgcactt
     1381 acttttcaaa ggtatgtgct gattttgaag ctgaactggt tgaaatggat ggcgaaccag
     1441 atcacgtttg ttaattaact atcctcccaa actggcgata tccagtctgg taaacagcct
     1501 caaaggcgta tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta
     1561 ctacaaaggc gttttgtgga gtcctggcta ttttgccagt agctgcggag gtgcgccaat
     1621 atccgccatc cgtcaataca ttgaacaaca gcaaacacct ggttaggtgg aaaaccgcgc
     1681 cgtatatccc cgccctgaag gacggggttt tacggcgcac cggataaaaa gcgccatcta
     1741 acgatggcgc gtctggaact actttttgtt aacgtcaaac atggtggcat cggtcgccat
     1801 gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt tattcaggtc
     1861 tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg catcaatcag
     1921 ctcaccttca aaatagaggc gagtatccat ggtacggtgg cctgtagcca tttgcgtccc
     1981 cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt tgctggtatc
     2041 tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg taaccactgg
     2101 tttacgctgc gctatcgctt ctttcatttc ggtgttggta tagttcaaaa ttttatccag
     2161 aactttctgc cctacctggg tagaaggttt tggtaccgga taataagtga ttgggttcca
     2221 gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta aaacaggttt
     2281 acttgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac cagaatattt
     2341 atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc ataaagctgc
     2401 aactttcgca aatgatgtgg tacg
//
LOCUS       DAZKFU020000417         2414 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZKFU020000417 DAZKFU020000000
VERSION     DAZKFU020000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.419"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..881
                     /gene="ycdU"
                     /locus_tag="ACHOQQ_RS24020"
                     /old_locus_tag="pgaptmp_004804"
     CDS             <1..881
                     /gene="ycdU"
                     /locus_tag="ACHOQQ_RS24020"
                     /old_locus_tag="pgaptmp_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HGU4472941.1"
                     /translation="FTFPSEDIGVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWF
                     KKKQNHKIELFLTQPLVAGFLSLIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLF
                     RMIRTSYVKQVAISVAKKVKYKSLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQW
                     LTFKSIIALLILCGVVLAINLFFLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKP
                     LWLRLFILLVIEVMWITLVSVLATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHND
                     FMMACDMYFRWGHFNK"
     gene            1248..>2414
                     /locus_tag="ACHOQQ_RS24025"
                     /old_locus_tag="pgaptmp_004805"
     CDS             1248..>2414
                     /locus_tag="ACHOQQ_RS24025"
                     /old_locus_tag="pgaptmp_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4472942.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAW"
BASE COUNT          595 a          477 c          627 g          715 t
ORIGIN      
        1 gatttacttt tccatcagag gatattggtg tcgttagtgg gattctggcc ctggtgctgt
       61 ataattttat ttttgatctc aaggcgtact gggcttataa atgcgtcacg aagaatatcg
      121 atttttcgtg gtttaagaaa aagcagaacc acaaaataga attatttctt acgcaacctc
      181 tggtggcagg atttctgtcg ttaatcatgt tgagtgcaat gagttggggg ctataccagc
      241 gtctaccctc gttatatgcg ctgttcctga tttcgttact tgggccgttg gttatctttc
      301 tgttgtttcg gatgatccgc accagttatg tcaagcaggt cgctatttca gtggcgaaaa
      361 aagtaaaata taaaagtctg actcgctatg tgctgctttc ggtgtgcatc tcaacggttg
      421 ttaacttgct tactatcagc ccgttgcgta acagtgattc ttttgtgata gaggggcagt
      481 ggttaacgtt taaatcgata attgcattgc tcattctttg tggcgtagtg ttggcgatta
      541 atctgttttt tctgcgcttc tccaggcggt acgctttttt gggcaggctt tttttgcagg
      601 aaatcgatct ctttttctcc agtgaaaatg cgttgtcgac cttttttgcc aagccgcttt
      661 ggcttcggtt attcatattg ctggttattg aagtgatgtg gattacgctg gtgtcggtat
      721 tggcaacgct tgtggaatgg cggatttggt ttgaagccta ttttttactc tgctatgtac
      781 cgtgcttaat ttactatttt ttctattgtc gattcctctg gcataacgat tttatgatgg
      841 catgtgacat gtatttccgt tgggggcatt ttaataagtg aggaagtgat aggaagtgac
      901 cagataatac atatatgttc tgtactctct tgcgcatttt gattgttgac tgagcaacca
      961 gacagttgat gtgcacgatt tcccctcgcc ctaacagacg tgggcggggg cacccggtgt
     1021 gtgcaatata cgaaaaaaaa gcccgtactt tcgtacgagc tcttctttaa atatggcggt
     1081 gagggggacc gcctgaaata aatctgactt aatccagggg tgttatataa tttcattata
     1141 aacaatgcat tacacttttt gttgtccgat attatctaat gtattcttag tgaagcgaga
     1201 gactaatggc atactggatg gcataccaga gtgacgggaa ttagcgtatg gcagtattga
     1261 cggatacgaa agcaagacat atcaaacctg atgacaaacc attgccccat gggggaatta
     1321 ccggactgac ccttcatcct tcttcagtaa aggggcgggg gaaatgggtt tttcgttatg
     1381 taagtccggt gacacaaaaa agacgtaatg caggattggg aacttaccca gaggtcagta
     1441 ttgctgaagc tgcacgtact gcccggataa tgcgagagca acttgctgca ggtgatgatc
     1501 ccctggagat taaaaaggct gaatctgaga aagtcgttat cccaacattt gccgatgcag
     1561 ccaggcgtgt acatgcagaa ctgtctcctg gatgggaaaa tccaaagcat gtaaggcagt
     1621 ggttatcgac gcttgagaat tacgcgtttc ctcaactggg agcaaaaacg ctggattcga
     1681 ttacggctgc ggacgtggca gaaacactgc gtccagtctg gttaaccttg tcagaaacgg
     1741 caagccgggt taaacagcgc attcatgttg ttatgcagtg gggatgggcg cacggttttt
     1801 gtgtggcaaa tcctgttgat gtggttgacc atttgcttcc tcagcagaca agaggacgtg
     1861 atgaacacca accagcaatg ccctggaggc agttaccgct ttttgtggcg accagtgtgt
     1921 atagtgatga accttataat gttacccgcg cactgttatt aatggtgata cttacagcaa
     1981 ctcgctcggg cgaagccagg ggaatgcgct gggctgaaat tgattttcat aagcgggtat
     2041 ggactatacc tgcagaaaga atgaaagcca ggctacagca tcgtgttcct ttatcccggc
     2101 aggctattta cattctggaa aatatacgtg gcctgcatga tgaactggtg ttcccttcac
     2161 ccagaaagca gcagatcctt tccgatatgg tgttgacaag ttttctgcgt aaaaagaaag
     2221 ccgtcagtga cattccgggg cgagttgcca cggcacatgg ttttcgctca acattcaggg
     2281 actggtgtag tgaacagggg tattcgcggg atctggcgga aagggcgctc gctcatacgc
     2341 tgaaaaataa ggttgaggcg gcatatcatc gtactgatct actggagcag cgtgtaccga
     2401 tgatgcaggc atgg
//
LOCUS       DAZKFU020000418         2386 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZKFU020000418 DAZKFU020000000
VERSION     DAZKFU020000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.420"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(97..1509)
                     /gene="pykF"
                     /locus_tag="ACHOQQ_RS24030"
                     /old_locus_tag="pgaptmp_004806"
     CDS             complement(97..1509)
                     /gene="pykF"
                     /locus_tag="ACHOQQ_RS24030"
                     /old_locus_tag="pgaptmp_004806"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGU4472943.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1517..1711)
                     /locus_tag="ACHOQQ_RS24035"
                     /old_locus_tag="pgaptmp_004807"
     CDS             complement(1517..1711)
                     /locus_tag="ACHOQQ_RS24035"
                     /old_locus_tag="pgaptmp_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472944.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2066..2275
                     /gene="fumD"
                     /locus_tag="ACHOQQ_RS24040"
                     /old_locus_tag="pgaptmp_004808"
     CDS             2066..2275
                     /gene="fumD"
                     /locus_tag="ACHOQQ_RS24040"
                     /old_locus_tag="pgaptmp_004808"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HGU4472945.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          627 a          518 c          577 g          664 t
ORIGIN      
        1 atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc gcccatcagg
       61 gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa cagatgcggt
      121 gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacaacgt cacctttgtg
      181 tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat cagtagaagt
      241 gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat gagccgtttt
      301 ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta ctgcgcgagc
      361 agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt cagcagtttc
      421 aacggcacca cggcataccg cttcggtaat gcgcagttta cggttgtcat tgttgaactc
      481 aagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga tagaaaccgc
      541 ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt cagtaccgtc
      601 gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt ttttgatcat
      661 ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac atttttcgat
      721 catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca ggtcgccacg
      781 cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga ggccttcctg
      841 gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg
      901 gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt ctacgccttg
      961 ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca gggcaatgga
     1021 aacgccaggc aggttcacac ctttgttttc acccaggtcg ccgttgttca gcactttaca
     1081 gataactttg ttaccttcaa tggcagtaac ttccataccg atcagaccat cgtcaaccag
     1141 tacggtgttg ccaacagaca ggtcagttgt gaaaccttca tacgttaccg caaccatttc
     1201 gctgttgccg ataacagatt tatcagtagt gaaagtaaag gtctgaccag ctttcagaga
     1261 aacgtcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg tatcaagcag
     1321 gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa tgcgctgacc
     1381 gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc cagcgtccag
     1441 cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa caattttggt
     1501 ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt gctttctggg
     1561 cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct ctaaggatag
     1621 gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgcc
     1681 tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag agtcaattgg
     1741 cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat
     1801 acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa aaggttacgc
     1861 aaaaacttcg ctgtttgcgc aacgtctggt gccggggttg tcactgtgaa gataagtgaa
     1921 agttcatccg gtgtataaat acatcatcga tcaattgcta cagttaaaat aatttatcgc
     1981 gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat atgctgatag
     2041 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
     2101 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
     2161 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
     2221 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
     2281 caggatgagg cgataagctt catcctgata ttcttgcttc agttgcggtc cagttaatca
     2341 ttctaaataa caactgctga aattatacga atttcgatat gtgttt
//
LOCUS       DAZKFU020000419         2373 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZKFU020000419 DAZKFU020000000
VERSION     DAZKFU020000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.421"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..342)
                     /locus_tag="ACHOQQ_RS24045"
                     /old_locus_tag="pgaptmp_004809"
     CDS             complement(<1..342)
                     /locus_tag="ACHOQQ_RS24045"
                     /old_locus_tag="pgaptmp_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HGU4472946.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGI"
     gene            complement(599..1234)
                     /gene="msrQ"
                     /locus_tag="ACHOQQ_RS24050"
                     /old_locus_tag="pgaptmp_004810"
     CDS             complement(599..1234)
                     /gene="msrQ"
                     /locus_tag="ACHOQQ_RS24050"
                     /old_locus_tag="pgaptmp_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGU4472947.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALIGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(1235..2239)
                     /gene="msrP"
                     /locus_tag="ACHOQQ_RS24055"
                     /old_locus_tag="pgaptmp_004811"
     CDS             complement(1235..2239)
                     /gene="msrP"
                     /locus_tag="ACHOQQ_RS24055"
                     /old_locus_tag="pgaptmp_004811"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGU4472948.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLHSWFKGNDRPPAPAGKPLDFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
BASE COUNT          625 a          566 c          574 g          608 t
ORIGIN      
        1 aatgccgtcc tcaatgccaa tcatctcgat atctgttgca taacctttgt gataataatc
       61 tttaatttca gcaaatgttt tagttttatc tgcatccgct ttcttctgaa agacggggtc
      121 aagtttgcca ctttgcagta aaggataaac ggattgccag actccatccc agtcactgag
      181 cgttcggttt tgtacattgg catcatcaaa aacaccatta gcagcttttt gttcgacctc
      241 tgttaagggt ttgccgtgtg agtgatgacc atgcgaaaag gcaggagcgc taacaataaa
      301 gacacctaaa gcaacagcca gtttgtgaag acgaatcgcc aaaacagttt tctccagagt
      361 cattgtatat gatgtgtaat gttatattgt aacatatgac aaaatgcaac aactcttagg
      421 agatgagagc atagttttct caggtgtact tatctatctt attgaaataa aatgaattgt
      481 gttgataatt tgaggttgat gacaaacgaa acataacctg atgcgatctg cgtaggccgg
      541 atagggcgct cgcgccacat ccggcaggga taacgcgtac tttgtcggca atatgaagtt
      601 atacagataa tttgttgtga acctgcttgc gtaaccggtt aaaaagggaa cgcaacttct
      661 tataccgtaa ggctaaaagc agtacagcca gcccagcgta gatgagcggc tgcggtgaaa
      721 taatcttcac cgaccacaga taatgtattg gggccaggat cgcgacaaga tagacgaaat
      781 tatgcaactg ttgccagtgc ttgccgagtt ttcgctgcat cgcctgggtt gaagtaaacg
      841 ctaaagcaag caaaataacc cagctgataa ttcctaacgt taaataaggc cgggtaatta
      901 actccttgcc tattaacgcc agattattca cgcccagctc cagcaatgcg taactggtta
      961 aatgcagtgt cgcccaggca aagcaccata agcctaacag gcggcgagtg cgtatcaata
     1021 acggctgttt tgcgtagcgt gccagaggtg tgattaacaa ggtcgccagc aaaaatttca
     1081 gtgcagtgcg accggtaaaa tgctgaatat ctttcactgg atcggcaccc agtccaccgt
     1141 gattgatcgc ccagaccagc cagagaaacg gcaacaatcc ggcaagatgc aggcaaactt
     1201 tcagccatgt cacctgtttt gctgtcagac gcatttagaa gttctcccgc agatccaggc
     1261 cacgatacag cgatgccacc tggtcggcgt aaccattaaa cagtagcgtt ggctggcgct
     1321 gtacatcgag gatgccgcct gaaccaataa atcgttcggt agcctgtgac cagcgtgggt
     1381 gatcaacatg cggattaacg ttggcgtaaa aaccgtattc gtcaggcgct gccagattcc
     1441 aggtggttgg cggacgctcg cgggtcagct taatactgac gatcgattta atccctttaa
     1501 agccatattt ccacggcaca atcagtcgca ccggcgcgcc attttgtggc ggtaacgcct
     1561 tgccataaac acctacggtc atcagtgtga gcggatgcat tgcttcgtcg agacgcaatc
     1621 cttcgacata aggatatttc agcccgccgc cgataaagcg gtcctgctgg cctggcatct
     1681 gttccggtgc ataaattgtt tcgaaagcga catacttcgc attgctggtg ggttcggcaa
     1741 gcgccagcaa tttgtgcagt ggaaaaccaa tccacggcac caccatcgac catgcttcta
     1801 cgcagcgcat acgataaata cgctcttcca gcgggaagcg acgggttaag tcatcatgat
     1861 cgagggtcaa tggttttgcc acttcgccgc tgattttcag tgtccatgga tcggttttca
     1921 ggctaccagc attagcggcg ggatcggctt tatccagccc gaattcatag aagttgttat
     1981 aaccggagac tttatctgct ggcgtcagtg gcaggttatt ttgccaggcg gcaggcttgc
     2041 tgaagtccag cggttttccg gcgggggcgg gcggacgatc gttcccttta aaccagctat
     2101 gcagatcggc atgtgcagcg tggggcaaag aaagtgcagc tgcgctgatg cccagtgctt
     2161 ttaacacctg ccgacgcttc ataaagaata ccgactcggc cgtaacatct gattctttta
     2221 aaaattgatt ctttttcata agccatcaca ctttttgtcg ttagccagca agagccgttt
     2281 actgttaatc tgtcagggag tgtcctccct aacagtagca gagatctgct tttacatatt
     2341 tatgcattaa ctgccacgat aggttgaata ccc
//
LOCUS       DAZKFU020000420         2353 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZKFU020000420 DAZKFU020000000
VERSION     DAZKFU020000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.422"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..499
                     /locus_tag="ACHOQQ_RS24060"
                     /old_locus_tag="pgaptmp_004812"
     CDS             <1..499
                     /locus_tag="ACHOQQ_RS24060"
                     /old_locus_tag="pgaptmp_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4472949.1"
                     /translation="SWVKDSLLHFLNGELVKAATAFRRGFAANTYIAEILCGNLHPFP
                     LAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAEIIKCAEML
                     MQEDDFEICESILRQQEKLRERIGETLSEKIVQKCRNMNGEYVWPWILPFSAAGMKHT
                     GIQYQ"
     gene            642..871
                     /locus_tag="ACHOQQ_RS24065"
                     /old_locus_tag="pgaptmp_004813"
                     /pseudo
     CDS             642..871
                     /locus_tag="ACHOQQ_RS24065"
                     /old_locus_tag="pgaptmp_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(858..>2353)
                     /locus_tag="ACHOQQ_RS24070"
                     /old_locus_tag="pgaptmp_004814"
     CDS             complement(858..>2353)
                     /locus_tag="ACHOQQ_RS24070"
                     /old_locus_tag="pgaptmp_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4472950.1"
                     /translation="RKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVLLLF
                     LLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEFTGM
                     IPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVGTTD
                     TLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTPWLN
                     TAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISNQGK
                     LGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFLHMI
                     GSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAMLYF
                     SDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQWLT
                     GIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRGK"
BASE COUNT          661 a          542 c          548 g          602 t
ORIGIN      
        1 atcgtgggtc aaggacagcc ttctgcattt tcttaatggg gaactggtaa aagctgcaac
       61 tgcatttcgt cgcggctttg cggcaaatac ttacatcgca gaaatactgt gcggaaatct
      121 gcatccattt ccacttgctg tatggcataa cttttcaggc ggtccggata ctgctgaaga
      181 ttattatgcc acataccacc cgttatgggg gcaatatccg gaagcattgc tgtttgtaaa
      241 ctggctctat aatcattcct ctgtgctgca tgaacgggca gaaatcatta aatgcgccga
      301 aatgctgatg caggaagatg atttcgaaat atgcgaaagt attttaagac agcaggagaa
      361 gttgcgtgaa agaattggtg agacgctttc tgagaaaatt gtacagaaat gcagaaatat
      421 gaatggtgaa tatgtctggc cctggatatt gccgttttca gcggcaggca tgaaacatac
      481 tggcatacag tatcagtaga tattgcatta gtgtatcctg cacacaagta ataatttatc
      541 caccaataat aacactgtta atgtcccctt cccctggttg tcagccaggg gttatcttct
      601 gaatatttct tttgaaaagg ataacacagt aaattatttt tatgaattat cccatggact
      661 cattaacacc ctttcataat gctttattgt caaacacgtt atggctgaca tcaaaaaaac
      721 cggatttcct ctgccagcgg gtaatcacct ccccggtgtt ttcggttggt ctggttactc
      781 cagtctggta attagcaaga taatcgatat aaacagtgga aaactcatcg tacataatct
      841 ggtgatgaac attacgctta ttttcccctg accggaagaa tcagaggctg cggtttcaga
      901 ctgtctgccg gtacattcct ctccccgtta aaaaccataa cgggttcatt atcttcgtct
      961 gtcagcggat tgaatggcgg tatattttca gtacgaatgc cggtcagcca ctgaaaaata
     1021 cctgcgaaat gacgggcact gattttcctg ctgacggact gatgagacgt gatgtcactg
     1081 gcggtaataa tcaggggaac gctgtagcct ccctgcacat gaccatcatg atgaacagga
     1141 ttggcactat cgctgaccga cagcccatgg tcagaaaagt aaagcatggc gaaatgacgg
     1201 gaatgccggc gaaggatacc atcaagctga ccgagaaagt tatccagttt actgatgctg
     1261 gcgaggtaac aggcaatctt tcggggatac tgctccaggt aatgattcgg ccaggagtta
     1321 agtcggtcac acggattcgg atgagagcca atcatgtgca ggaaaatcac cttcggagag
     1381 gatttatccg ccagcgcacg ttctgtttcc tgtaacaaca acatgtcatc cgttttacgg
     1441 gaagcaaagc tgcctttctt gaggaaaacg gtatgctccg catcagaagc aataacagag
     1501 atgcgtgtat catgctcccc cagttttccc tgattggata tccaccatgt gctgtatcct
     1561 gcttttgctg ccagcgctac cacgttgttg ccggagtcag ggttctgctc atagtcataa
     1621 atcagtgtcc ggctcaggga aggtacggta ctggctgctg ccgatgtata gccgtcaata
     1681 aataaaccgg gagccgtatt cagccacggt gtggttggta cgggataacc atataccgac
     1741 atataatccc tgcgcacact ctcaccagtg acaatcacaa tcgtgtcata caacggtgct
     1801 cccggcagga ttttccagct gtcagccctg tgctgactca gttgttcata acgctgcatt
     1861 tcacgcaatg tgtcagttgt ccccacaacg gttcctttaa ccatccgcaa cggccagctg
     1921 tttacagagc ataatacgaa cagcagcagt gccagccagt tacggtgacc gcggtggtgt
     1981 gtccgccaga aaataaccat gaacaccaga atcgcggcac tgaccagaaa atgataaaca
     2041 ggaatcatcc cggtaaactc cgcagcctca tccgccgtgg tctgcaacaa cgcgacaata
     2101 aaactgttgt tgattttacc gtacgtcata cccgcaggcg catacagtgc acaacagaac
     2161 agaaataaca gagctgtaat ggatgtaagg gtatttctgt gtgcaaggag cagaagaagg
     2221 aacagcagca acacattccc ggtggcattc ttctcagtgt atccgcatgc aattgtggtt
     2281 atgacagaaa taacaaaaaa gaataaaaac aataaaatcc gtggtgtgtt gtccggacaa
     2341 aacagttttc tga
//
LOCUS       DAZKFU020000421         2330 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZKFU020000421 DAZKFU020000000
VERSION     DAZKFU020000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.423"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            344..907
                     /locus_tag="ACHOQQ_RS24075"
                     /old_locus_tag="pgaptmp_004815"
     CDS             344..907
                     /locus_tag="ACHOQQ_RS24075"
                     /old_locus_tag="pgaptmp_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4472951.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            904..>2330
                     /locus_tag="ACHOQQ_RS24080"
                     /old_locus_tag="pgaptmp_004816"
     CDS             904..>2330
                     /locus_tag="ACHOQQ_RS24080"
                     /old_locus_tag="pgaptmp_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4472952.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLLYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLVEAGIPDGIVVGISQGWKLGGA"
BASE COUNT          523 a          561 c          754 g          492 t
ORIGIN      
        1 ctgccggagg tgtccggagg tagtggcaga gcggacgaca tcatcggact gtaacccgac
       61 cggggggggt atcatccggc gtaaaaaacg atcgctccgg acaccgcgcc ccctctcacg
      121 cagagaaaaa attcccgttt cagggcagtt aacatgttaa ctggctgtcc gggcattttt
      181 gcggttttta tctttattat tcagtttgtt gcgtggaaaa aatgttaaca ggctttttca
      241 gcaaatgtta accaggcagc agttaacatt tgcggcatga gacgccggga aaaatgggct
      301 gaaccatacc cggctgagtg cgttctggac ccgggaggag gctgtgctga caacgcaaaa
      361 acgaaaattt gcgctggcgc tcatgtccgg gaaaaacaaa acagcgtcag ccattgccgc
      421 cggttattcg gcgaagacag ccagggttaa aggctcgcag ctggcaaaag atccggaggt
      481 gcttgcgttt atagcccgta aacagtgcga gacggtggag gtggatgagg ttcctgttta
      541 ccggcagaaa aaatcagagc aggaggataa accccgtcgc cgtgaggcgg ctgcaatacc
      601 acagccggac gaaacaaatc cggagatgcc accgcccgtg gtgatatctc ctggtattga
      661 gtatatggag gacggtcttc ccgatccggt gaaagcgatg gggcgtcttc tggtggagaa
      721 cattaatacc gaccccaggc tggcgctgga tgcggcttat aagctggcgc agttcacgca
      781 ccacaaaaaa ggggatgccg gtaaaaaatc ggcaaaaggt gacgcggcga aaaaagcggc
      841 taaccgtttt gcggtgccac caccaccccg actggtggtg aataatgata atgagggcaa
      901 cggatgatac ctgtgtggag cacggcctgc ccggactggg cagagcgcct gaaaaagggg
      961 ctgtcgatta ttccggcccc gatttatccg gatcaggctg cacatgcact ggcgattttt
     1021 aaacaactgc ggattgtgga tgcaccgggc agcccgacgt tcggggagtc ctgtgcaccg
     1081 tgggtgtttg acctggtggc ggccctgttt ggctcctacg atgcgcagac cggtgtacgc
     1141 catatcaagg aagtttttat ccttatcccc aagaaaaaca gcaagtccac gctggctgcc
     1201 gggatcatga tgacggcgct gttactgaac tggcggcagg cggcgggcta caccattctg
     1261 gccccgaccg tggaggtggc ggctaacgcc tttaatcctg cgcgggatat ggtacgacgg
     1321 gacgatgatc tggatgacct ctgtcaggtg cagacacata tccggaccat cacccatcgg
     1381 gtgacggaca ccacccttaa ggtggtggct gccgatccga atacggtatc cggtatcaag
     1441 tccgtgggga cgctgattga tgaactgtgg ttatttggca agcagtacaa agcggaggac
     1501 atgttacgtg aagccatagg tggccttgcc tcccgcccgg aagggtttgt ggtgtatacg
     1561 accacccagt cgaatgaacc gcccgccggg gtgttcagac agaaactgct gtacgcccgg
     1621 gatgtccgtg acggcaaaat tcatgatccg cactttctgc cggtgatatt tgaacaccct
     1681 cctgaaatgg tggaaagcgg ggctcacctg ctgatggaaa acctcgccat ggtcaatccg
     1741 aatctcggtt attcggtgga tgaggctttt ctgtaccggg agtaccgtaa agcccgggag
     1801 gctggtgagg aagcatttcg tggcttcatg tcaaaacatg ccaatgtgga aattggtctt
     1861 gccctgcgtt ctgaccgctg ggcgggtgcg gatttctggg agcagcaggg caggcgcgtc
     1921 agcctggacg atatcctgca gcgcgctgat gtggtgacgg tggggattga cggcgggggc
     1981 ctggatgatc tgctgggaat gtacgtgatt ggccgtgaca gggaaacccg cgaatggctg
     2041 ggctggggcc atgcctgggc gcatgaaacc gcggtggtca gacggaagag tgaggcatcc
     2101 cggtttcagg attttgtggc ctgtggagat atgacgattg tccgtcgggt cggggatgac
     2161 acggcggaag tggcggagta tgtgcgtcgt attcatgagg ctgagttact ggagcatatc
     2221 ggtattgacc cgtcaggtgt ggggcagatt ctggattcac tggtggaagc cgggatcccc
     2281 gacggaattg ttgtggggat aagccagggc tggaagctgg gcggggccat
//
LOCUS       DAZKFU020000422         2257 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZKFU020000422 DAZKFU020000000
VERSION     DAZKFU020000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.424"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(67..296)
                     /locus_tag="ACHOQQ_RS24085"
                     /old_locus_tag="pgaptmp_004817"
                     /pseudo
     CDS             complement(67..296)
                     /locus_tag="ACHOQQ_RS24085"
                     /old_locus_tag="pgaptmp_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(686..1495)
                     /gene="iolB"
                     /locus_tag="ACHOQQ_RS24090"
                     /old_locus_tag="pgaptmp_004818"
     CDS             complement(686..1495)
                     /gene="iolB"
                     /locus_tag="ACHOQQ_RS24090"
                     /old_locus_tag="pgaptmp_004818"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HGU4472953.1"
                     /translation="MTNLLSTCTTRAGNIQHISPQNAGWEYVGFDVWLLKAGESLTLP
                     SDDRERCLVLVAGLASVKAADHFFYRIGQRMSPFERIPAYSVYLPHHTEAKVTAETDL
                     ELAVCSAPGFGELPVRLISPQDIGVEHRGKGRNQRLVHNILPDTQPADSLLVVEVYTR
                     EGDTSSWPAHKHDTAVEGQETYLEETYYHRFDPPQGFSLQRVYTADRSLDECMAVYNR
                     DVVKVPKGYHPVATIAGYDNYYLNVMAGPLRKWQFTWEADHAWINSSEYPR"
     gene            complement(1520..>2257)
                     /locus_tag="ACHOQQ_RS24095"
                     /old_locus_tag="pgaptmp_004819"
     CDS             complement(1520..>2257)
                     /locus_tag="ACHOQQ_RS24095"
                     /old_locus_tag="pgaptmp_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGU4472954.1"
                     /translation="MPDADLDATVNAIMGGAFGSAGERCMALPVVVAVGDETANRLIE
                     RLKPLIAALRIGPGELREKDENEMGPVVSRMHQQKVLAYIDKGVSEGATLVMDGRNYC
                     VKGYPEGFYVGGTLFDNVTPEMTIWREEIFGPVLGIVRVTDYATAIRTVNSHEFGNGS
                     VIFTSNGHYAREFAQSVEAGMVGINVPVPVPMAFHSFGGWKRSVFGALNVHGPDGVRF
                     YTRMKTVTARWPNGQQIASEFSMPTLG"
BASE COUNT          597 a          567 c          515 g          578 t
ORIGIN      
        1 catgttggtg cttttattct gtgaggagtg ctggcatggc ggacacaacc atttgttctt
       61 tttagctgcc atttgataat attttcctga agctttccgg ggatatattc cccagacggg
      121 agtggcggcg ctgacggttg tagaatatct caatgtatac ccagtattac tgagatgact
      181 tcattccggc tactaaaatg gtagtggttc agactttcat tcttcagagt tccccaaaag
      241 ctttccatag gggttttgtc gtaacagttc cctttacgag acattgatgt tttcagagct
      301 aactgcgata gactccttca acttcaactt caacttcaac ttcaacttca acttcaactt
      361 caacttcaac tgagaaacgg atgatttcac gatagactgc ccaacgtatt gagcccgtta
      421 ggaagccaga gtaacaagcc gccgtgtacg gcatttctct cctaattcgg cagaaccaaa
      481 aatattgtca gctcaggagt aagaatcaga aaaaaccata acagaacacc agaaacagga
      541 tattatcaga cagagaaaaa attcaaaaaa atccaccgat tctgttgcct gtggatttgt
      601 gtataagagg cagaaccctg ttggggactg tgcacaaaaa agccagctgc agaggacaac
      661 tggcaaagga atatttaacg tattgttaac gcggatactc tgacgaatta atccatgcgt
      721 gatcagcttc ccatgtgaat tgccatttgc gtaatggccc ggccatcaca ttcagataat
      781 agttatcgta acctgcgata gtggccaccg gatgatagcc tttcgggact tttaccacat
      841 cgcggttata tactgccata cattcgtcca gactgcgatc ggcggtgtag actcgctgta
      901 agctgaatcc ctggggtgga tcgaaacggt ggtagtaggt ttcttccaga taggtttcct
      961 gcccttcaac tgctgtgtca tgcttatggg caggccagga acttgtatcg ccttcccttg
     1021 tatatacttc cactaccagc agactgtcag caggttgagt gtctgggagg atattatgaa
     1081 ccaagcgctg attgcgccct tttcccctat gttccacccc aatatcttgt ggtgagatca
     1141 gtcttaccgg aagttcaccg aatccaggag cactgcagac agccagttcc agatcagttt
     1201 cggcagtgac ttttgcttct gtatggtgcg ggaggtaaac agaataagcc ggaattcgct
     1261 caaaaggact catgcgctgg ccaatccgat aaaaaaagtg gtcagcggcc tttacgctgg
     1321 caagtccggc aaccagaacc aggcaacgtt cgcgatcatc ggatggcaag gttaaagatt
     1381 cgccggcttt caacagccag acatcaaaac cgacatactc ccagcctgcg ttttgcggag
     1441 aaatatgttg aatatttcca gccctggtgg tgcaggtact cagcaagttg gtcataatct
     1501 tctccttcct gaacgtttat taacctagcg taggcatact gaactcggat gcgatctgct
     1561 gaccgttcgg ccagcgtgcg gtaacggttt tcatccgggt ataaaaacgc accccatccg
     1621 gtccgtggac attcagcgcc ccaaacaccg agcgtttcca gccgccgaaa gagtgaaacg
     1681 ccattggaac cggtaccgga acattgatac cgaccatccc ggcttcaacc gattgagcaa
     1741 attcgcgggc ataatggccg ttggaggtga agatcacgct accattgcca aattcatgag
     1801 agtttaccgt acgaatggcg gtggcataat ctgttacgcg tacgatgccg agcacgggac
     1861 caaaaatttc ctcacgccag atagtcattt ccggcgtgac gttatcaaac aaggtaccgc
     1921 cgacataaaa tccttcagga tagcccttca cgcaataatt tcgcccatcc atcaccagtg
     1981 ttgctccttc gctaacgcct ttgtctatat atgcaaggac tttttgttga tgcatacgtg
     2041 aaacaactgg ccccatttca ttttcgtctt tttctcgtaa ctcaccgggg ccaatacgca
     2101 gcgctgcaat aagtggtttt aggcgttcaa taagacggtt ggctgtttca tcaccgacag
     2161 cgaccaccac aggtaatgcc atacaacgtt cgcctgcgga accaaaagcc ccacccataa
     2221 tagcgttaac ggtagcatca aggtcagcat ccggcat
//
LOCUS       DAZKFU020000423         2253 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZKFU020000423 DAZKFU020000000
VERSION     DAZKFU020000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.425"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..247)
                     /locus_tag="ACHOQQ_RS24100"
                     /old_locus_tag="pgaptmp_004820"
     CDS             complement(<1..247)
                     /locus_tag="ACHOQQ_RS24100"
                     /old_locus_tag="pgaptmp_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472955.1"
                     /translation="MRMFTNLLYDICTVFELFKEGESPRDKRKSTDFGAHQRFWDQRY
                     NELSHIIDAEGVYSLEQRRIIFSRYEYFYYMMNSYPVY"
     gene            348..596
                     /locus_tag="ACHOQQ_RS24105"
                     /old_locus_tag="pgaptmp_004821"
     CDS             348..596
                     /locus_tag="ACHOQQ_RS24105"
                     /old_locus_tag="pgaptmp_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4472956.1"
                     /translation="MSTQTGHFYTPSRILRMAELTRLLGISRSTIYEKMNPASKYYDA
                     TFPRPVRLGSSAVGWRASAIDEWLTLHTTPTRSVTKDK"
     gene            612..2177
                     /locus_tag="ACHOQQ_RS24110"
                     /old_locus_tag="pgaptmp_004822"
     CDS             612..2177
                     /locus_tag="ACHOQQ_RS24110"
                     /old_locus_tag="pgaptmp_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HGU4472957.1"
                     /translation="MTKLSPPELDITKLPDRCQALLDEMHEETGISREILLSIMLTVK
                     AASVQDTHEVELSGGQRTSLQIYMCLSSASGSGKTSACTKLIAPVHETEEELHQAYID
                     DKKNYDRMMEMWTTDKKILERRYKKEMERSPENAAAARAALEKCIANKPVPPVQQVLI
                     VNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVTVERASRP
                     GFRVKDSRLTMLILTQPVIFDKFFTLTGDQIRGNGFLARVLFCEPGANKIMTTEPHTA
                     TPVVTQQCATCFHEKSFGSQIRDSLRASRERRAKGEQRICMTLSNAASHDLEIFHKEN
                     MSAVRQNPRMTTFEDIIVRKREQAVRIAALLELENNPHGTVITRESINSAIYLVDFYF
                     QHLISKLESLREISPAEKLDKWLRENIIRVKGYEYQKSHILQYGPYALRNKCVLDEAL
                     DILAEQKKIVIDYSIGQKIIYIGDAITPCELANEANIPIMERGMFMVCWDHKLNKYRN
                     EEQTKIWNITTKN"
BASE COUNT          732 a          509 c          457 g          555 t
ORIGIN      
        1 aatacacagg gtaagaattc atcatgtaat aaaaatattc ataccgggaa aatataattc
       61 gccgttgttc cagactatac accccttcag catcaataat gtgactgagc tcattataac
      121 gttgatccca gaatctttga tgcgctccaa aatcagtact cttccgctta tctctcggac
      181 tctctccttc cttaaacaac tcaaaaactg tacaaatatc atacaaaaga tttgtaaaca
      241 tgcgcatctg ctctgatctc cggttggttt ccgttttatc acaggggaca ttctggcctt
      301 aacctagcaa attaatgaca tagccttcaa ctcacatgga gaacaatatg tcaacccaga
      361 ccggacattt ttataccccc tcacgcatcc tgcgtatggc tgaacttacc cggctccttg
      421 gtatatcaag atcgaccatc tacgaaaaaa tgaatccggc atcaaaatac tacgatgcca
      481 cctttccccg gcctgtccgg cttgggagtt cggctgtagg ctggcgggca tctgccattg
      541 atgagtggtt aactttacat acaacaccaa ccagatccgt aactaaggat aaataaggaa
      601 aactgtacgc catgacaaaa ttatcccccc ctgaacttga cataactaaa ctgcctgaca
      661 ggtgccaggc attacttgat gaaatgcatg aagaaacggg aataagccgt gaaatactgc
      721 tgtctattat gctgacggtc aaggctgcct cggtccagga tacgcatgaa gttgaacttt
      781 ctggagggca gcgtaccagt cttcagatat atatgtgtct ttcatcagca tcaggcagtg
      841 gaaaaacctc tgcctgcaca aaattaatcg cccctgttca tgaaacagaa gaagaactac
      901 atcaggcata tatcgacgat aaaaaaaact atgatcgcat gatggaaatg tggacaacag
      961 ataaaaaaat cctggaacgg agatataaaa aagaaatgga aagatccccg gaaaatgctg
     1021 ctgctgcacg agcagcgctt gaaaagtgta tagcaaataa acccgtacct cccgtacaac
     1081 aggttctcat cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac
     1141 cttctcttct gttgctgtct gatgaaggag gaactattct ggataagcgt ttcgaacgca
     1201 aatcggcgct gtacaacaca ttatggagtg ggcaaccagt caccgtagaa cgggcatcca
     1261 gacccgggtt ccgggtaaag gattcccgtc tcactatgct tatactcact caaccggtaa
     1321 tatttgataa gttttttact ctcactggcg accagatccg gggcaatggc tttctggccc
     1381 gggtgctatt ttgcgaaccc ggagcaaata aaataatgac gacagaaccg catactgcca
     1441 ctcctgttgt aacgcagcaa tgtgcaacct gttttcatga gaaaagtttt ggcagtcaga
     1501 tcagagactc tctgagagcg tcccgggaaa ggcgtgcaaa aggtgagcaa cgcatctgca
     1561 tgacattatc aaacgcagca tcccacgatc tggaaatctt tcacaaagaa aatatgagtg
     1621 cagtcaggca gaacccacgt atgaccactt tcgaagacat tatcgtcagg aaaagagaac
     1681 aggctgttcg aatagccgca cttctggagc tggagaacaa tccacatggt acagtaataa
     1741 cacgagaaag tatcaatagt gctatttatc tcgttgattt ttattttcag catcttatat
     1801 ccaaactgga atcacttcgg gaaatatctc ctgcagaaaa actggataaa tggctcagag
     1861 aaaatatcat ccgggtaaaa ggatacgaat atcaaaaaag ccatatacta caatatggtc
     1921 catatgcgct caggaataaa tgtgttcttg atgaagcact tgatatactt gcagaacaga
     1981 aaaaaatcgt aattgattat tcaatcggcc aaaaaatcat ttatatcggg gacgcgatta
     2041 caccttgtga attagcaaat gaggcaaaca tcccgataat ggagcgtgga atgtttatgg
     2101 tctgttggga ccataagctg aataagtaca gaaatgaaga acagactaaa atatggaaca
     2161 taactacaaa aaattaaagt tcccgtcttt tatcattcag ttaccaacct gaatatctga
     2221 aacaatgcca gacatcactt acagactggc att
//
LOCUS       DAZKFU020000424         2248 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZKFU020000424 DAZKFU020000000
VERSION     DAZKFU020000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.426"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..565
                     /locus_tag="ACHOQQ_RS24115"
                     /old_locus_tag="pgaptmp_004823"
     CDS             <1..565
                     /locus_tag="ACHOQQ_RS24115"
                     /old_locus_tag="pgaptmp_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4472958.1"
                     /translation="RLLENMVRTGVVSEVDLKNWRIRVTSGGLTTDWLRWNCTRAGAF
                     KIWLPPSPGEQVLLLCPGGSPEMAVVAGSLYSEEHPAPGTKQNEVILLAPDGAEIRYD
                     AEKGALTVKGIKTAEISAETRITLDTPAVECTKHLKVRTFELTDGGTLKGDITHSNGN
                     LRSNGVTVHTHVHNGVQSGGSMTGGPK"
     gene            562..927
                     /locus_tag="ACHOQQ_RS24120"
                     /old_locus_tag="pgaptmp_004824"
     CDS             562..927
                     /locus_tag="ACHOQQ_RS24120"
                     /old_locus_tag="pgaptmp_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HGU4472959.1"
                     /translation="MTPVCTGMNPHGSGCISDTEHLRHSVNNILTTPRGSRIMRREYG
                     SLLPELLDAPQNDVTRLQCMSAVVMALAQWEPRIAINRVDIAWLPDGRALLAISGVLT
                     RTMQPVQMSIPLRGSNADN"
     gene            914..1810
                     /locus_tag="ACHOQQ_RS24125"
                     /old_locus_tag="pgaptmp_004825"
     CDS             914..1810
                     /locus_tag="ACHOQQ_RS24125"
                     /old_locus_tag="pgaptmp_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4472960.1"
                     /translation="MPTIDLSQLPRPQIIESLDYESILKDVKAFMIDAFPENMRSAVS
                     AAIALESEPLNIIAQAIAFREMLLRQRINDAAAGCMLSHAVSTDLDNLAAIVNAKRLE
                     LKPATDTEAAIMESDSAFRLRAQSGFEGFSVAGPSAAYEYFARSASGLVADARATSPS
                     PATVIVSILSTEGDGTASPELIATVSRALNDETVRPLADRLTVQSATIVSYDIEAVLY
                     FYPGPESEPILAAARNALDTWLKTQNAIGRDVARSAIMATLHVPGVQRVELKKPAQNV
                     VISNTQSARCTSRNISAGGTDE"
     gene            1803..>2248
                     /locus_tag="ACHOQQ_RS24130"
                     /old_locus_tag="pgaptmp_004826"
     CDS             1803..>2248
                     /locus_tag="ACHOQQ_RS24130"
                     /old_locus_tag="pgaptmp_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HGU4472961.1"
                     /translation="MNNRLLPPSSPSFLHHSEQGAQRLSELPIHINTLWNPDTCPAHL
                     LPWLAWALSVDRWDETWSEEARRQIIRDSWHIHRHKGTISAMRRAVEPFGAILSITEW
                     WENNDPPGSFRLDIDISERGLTEETRQEMERMITTVRPVSRHLAAM"
BASE COUNT          531 a          663 c          622 g          432 t
ORIGIN      
        1 gcgactgctg gaaaacatgg tccgcaccgg cgtcgtttcg gaggtggatc tgaaaaactg
       61 gcgcattcgc gttaccagcg gcggactgac aacggactgg ctccgctgga actgcacacg
      121 cgccggggcg tttaaaatct ggctgccccc ctcccccggt gaacaggtgc tgttgctctg
      181 cccgggcggc agtcctgaaa tggccgttgt ggctggcagt ctctacagcg aagaacaccc
      241 ggcgcccggc acaaaacaga atgaagtgat actccttgca cctgacggtg ccgagatacg
      301 ctatgacgca gaaaaaggcg cgctgacggt gaaaggaatc aaaaccgcag agatctctgc
      361 ggaaacacgt attaccctgg acacaccggc cgtagagtgc acaaaacatc tgaaggtcag
      421 gacgtttgaa ctgactgacg gtggcaccct gaaaggggat atcacacaca gtaacggaaa
      481 cttacgctca aatggtgtta ccgtgcatac gcacgttcat aacggtgttc agtccggcgg
      541 aagcatgaca ggggggccga aatgacaccg gtatgcacag gcatgaaccc gcatggtagc
      601 ggatgcatca gtgacacaga acacctccgc cactctgtaa acaatattct gaccaccccg
      661 cgcggcagtc gcatcatgcg aagagagtat ggcagcctgt tacctgaact gctcgatgcg
      721 ccacaaaacg acgtgacgcg tctgcagtgc atgagcgcgg tggttatggc gctcgcacag
      781 tgggagcccc ggatcgccat taaccgggtg gatatcgcct ggctgcctga cggacgcgct
      841 ctgctggcca tctcaggcgt gctcacccgc acaatgcagc ccgtacagat gagcattccg
      901 ctgaggggga gtaatgccga caattgacct gtctcagtta ccacgtccgc agatcattga
      961 atcactggat tatgagagca tactgaaaga cgtgaaggcc tttatgattg atgccttccc
     1021 tgaaaacatg cgcagtgccg tcagcgcagc cattgcgctg gaatcagagc cactcaacat
     1081 cattgctcag gccatcgcct ttcgcgaaat gctgctccgt cagcggatca acgatgcagc
     1141 ggcggggtgc atgctcagcc atgccgtatc cacagacctg gataatctcg ccgctatcgt
     1201 gaatgcgaaa cgccttgaac tgaaacctgc tactgataca gaagctgcca tcatggagag
     1261 cgacagcgcg ttccgtctgc gggcacaatc cggatttgaa ggtttcagcg tcgcaggccc
     1321 ctctgctgca tatgaatatt ttgcccgcag tgccagcggt cttgttgccg atgcacgggc
     1381 aaccagtccg tcacctgcga cggtcattgt ctccattctc tcaacggaag gagacggcac
     1441 agcgtcaccg gaattaatcg cgaccgtcag ccgggctctc aatgatgaaa ctgtgcgccc
     1501 cctggccgac agactcacgg tacagagtgc gaccatcgtc agttacgata ttgaggccgt
     1561 actctatttt tatccgggcc cggaatccga acccattctt gccgccgcca gaaatgcact
     1621 tgacacatgg ctgaaaactc agaatgccat aggacgtgat gtggcccgct ctgccattat
     1681 ggcaacgcta cacgtaccgg gcgttcagcg agtcgagctg aagaaaccag cgcagaatgt
     1741 ggtgatcagt aacacccagt cagcccgctg cacatcccgg aacatctctg caggtggcac
     1801 cgatgaataa ccgactgctg ccgccatcct cgccctcctt cctgcatcat tcggaacagg
     1861 gcgctcagcg cctgtcagag ctgccgatcc atatcaacac cctctggaac cccgacacct
     1921 gcccagccca tctgctgcca tggcttgcct gggcgctttc cgttgaccgc tgggatgaaa
     1981 catggagtga agaggcacgt cgtcagatca tccgtgactc gtggcatatt caccgccaca
     2041 aaggcaccat cagcgccatg cgtcgggctg tggagccctt cggcgcgata ctgagtatta
     2101 cagaatggtg ggagaacaac gatccgccgg ggtcattcag gctggatatt gacatatcgg
     2161 agcgcggact gacagaggaa acccggcagg agatggagcg aatgattacc accgttcgcc
     2221 cggtaagccg acaccttgcc gccatgaa
//
LOCUS       DAZKFU020000425         2242 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZKFU020000425 DAZKFU020000000
VERSION     DAZKFU020000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.427"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(12..284)
                     /gene="rcnR"
                     /locus_tag="ACHOQQ_RS24135"
                     /old_locus_tag="pgaptmp_004827"
     CDS             complement(12..284)
                     /gene="rcnR"
                     /locus_tag="ACHOQQ_RS24135"
                     /old_locus_tag="pgaptmp_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGU4472962.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            405..1229
                     /gene="rcnA"
                     /locus_tag="ACHOQQ_RS24140"
                     /old_locus_tag="pgaptmp_004828"
     CDS             405..1229
                     /gene="rcnA"
                     /locus_tag="ACHOQQ_RS24140"
                     /old_locus_tag="pgaptmp_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGU4472963.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVRAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1448..1786
                     /gene="rcnB"
                     /locus_tag="ACHOQQ_RS24145"
                     /old_locus_tag="pgaptmp_004829"
     CDS             1448..1786
                     /gene="rcnB"
                     /locus_tag="ACHOQQ_RS24145"
                     /old_locus_tag="pgaptmp_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGU4472964.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1868..>2242)
                     /locus_tag="ACHOQQ_RS24150"
                     /old_locus_tag="pgaptmp_004830"
     CDS             complement(1868..>2242)
                     /locus_tag="ACHOQQ_RS24150"
                     /old_locus_tag="pgaptmp_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4472965.1"
                     /translation="TINSKQGDIILRCKNLLGQQNQTSRRMQVYLSSSDLLAGSNTIL
                     KGAEDNGVGFILESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYV
                     YDTNKIKSGALEATALINVKYD"
BASE COUNT          568 a          535 c          498 g          641 t
ORIGIN      
        1 ataactccta tttatttgat atatgaatcc agcaccttca gaacgacatc cagatcttct
       61 tcacgtttta gctcatcccc ctggtgaacg atgtgttccg tcagatgacc tttaatcact
      121 tcccgcatca gaccgtttac cgcgccacgg atagcagcaa tctgttgtaa aactgcagcg
      181 cattcgtgcg gctcgtcgag cattttcttg agcgccacga cctggccctg aatcttactg
      241 gcacgcgctt tcagtttctg tttatcacgg attgtatgag acatggcaac acctggttaa
      301 caagaatatg aaaaatcata gcactattaa tctactgggg ggtagtatca ggtactgggg
      361 gggagtagaa tcagattgcc gaattaatac taagaattat tatcatgacc gaatttacaa
      421 ctcttcttca gcaaggaaac gcctggttct tcatccccag cgccatctta cttggtgcgc
      481 ttcatggcct ggaaccaggg cactcaaaaa cgatgatggc ggcgtttatc atcgccatca
      541 aaggcaccat taaacaggcg gtaatgctcg gactggcagc aaccatttcg cataccgcag
      601 tggtctggtt gattgccttt ggcgggatgg tgatcagcaa gcgctttact gctcaatcag
      661 cagaaccgtg gctccagctg atttccgcag tgatcattat tggcaccgcg ttctggatgt
      721 tctggcgtac ctggcgcggc gaacgcaact ggctggagaa tatgcacgag catgatcatg
      781 aacatcatca ccacgatcac gaagatcacc acgaccatgg acatcatcac catcacgaac
      841 atggcgagta tcaggatgcc catgcacgag cccatgccaa tgacattaaa cgacgctttg
      901 atggtagaga ggtcaccaac tggcaaattt tgttatttgg cttaaccggt ggccttatcc
      961 cctgcccggc agcaattacc gtgctgttga tatgcattca gttgaaagcc ctgacactgg
     1021 gcgcaacact ggtcgtcagt ttcagcattg gcctggcgtt aacgcttgtc accgtaggcg
     1081 ttcgcgcagc aatcagcgtt caacaggtcg caaaacgctg gagcggattt aacactctcg
     1141 ctaaacgcgc cccctatttt tccagtctgt tgattggctt agttggtgtg tatatgggcg
     1201 tacatggctt catgggcata atgcgataac tcaatccttt aggcttgcat gttatcttca
     1261 gcccaggatg attcttaaat cagctatttc cgctgactct ctgctcgata aggatgcttt
     1321 tagggcatcc tttcatttac actttttacg aaatcatggg atcactaaca aaatatcgct
     1381 tgtcagttat attgtatggc aggaaagata tgcgactgat attacagatc cccaaagtgg
     1441 agagtttatg accattaaaa ataagatgtt gctgggtgcg cttttgctgg ttaccagtgc
     1501 cgcctgggcc gcaccagcca ccgcgggttc gaccaatacc tcgggaattt ctaagtatga
     1561 gttaagcagt ttcattgctg actttaagca tttcaaacca ggggacaccg taccagaaat
     1621 gtaccgtacc gatgagtaca acattaagca gtggcagttg cgtaacctgc ccgcacctga
     1681 cgccggaacc cactggacct atatgggtgg cgcgtacgtg ttgatcagcg acaccgacgg
     1741 taaaatcatt aaagcctacg acggtgagat tttttatcat cgctaaaaaa agccccctca
     1801 tcatgagggg gaaatgcaga caccttgtta ttttttatta ttagccactt gctcgtcttg
     1861 cttgttatta gtcgtatttc acgttgatta atgcggttgc ctccagtgcg ccagatttaa
     1921 ttttatttgt atcgtagacg tagtaactgg cagttatcgg aattgaaacc gttgtatctg
     1981 taagttttga ctttgccgca atttctttta aatttgtgta tcctttactg ctgttagtga
     2041 tatttaaaag tgtgactggc gaaccattac tttcaagaat aaatccgact ccattatctt
     2101 ctgcaccttt caggatcgtg ttactgccgg ctaacaaatc agaacttgat aaatacacct
     2161 gcattctccg tgatgtttga ttctgctgac ctaataaatt tttacagcgc agaataatat
     2221 ccccttgttt actatttatt gt
//
LOCUS       DAZKFU020000426         2240 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZKFU020000426 DAZKFU020000000
VERSION     DAZKFU020000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.428"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(246..1118)
                     /locus_tag="ACHOQQ_RS24155"
                     /old_locus_tag="pgaptmp_004831"
     CDS             complement(246..1118)
                     /locus_tag="ACHOQQ_RS24155"
                     /old_locus_tag="pgaptmp_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4472966.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1384..>2240
                     /locus_tag="ACHOQQ_RS24160"
                     /old_locus_tag="pgaptmp_004832"
     CDS             1384..>2240
                     /locus_tag="ACHOQQ_RS24160"
                     /old_locus_tag="pgaptmp_004832"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HGU4472967.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSGYMEAGDDSICYAKLDDSVITDDIKDDAIRTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNKHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEP"
BASE COUNT          596 a          552 c          573 g          519 t
ORIGIN      
        1 gaggtccggc tgtgggagtt tctgaattgt ggttctgttg ttccctgaat gtctttctgt
       61 acgtatctgt gcatgttact gacaaaggtt attattagcc tatcgatcga tataaacgat
      121 cgataaaata gatatcgatc gttttattct attattgagc tattgcacgg acaagagaga
      181 cagtaacgaa aacagtgctt ttgtgagcag actcagagtc agggagggca tcagtcacga
      241 caggttcaga agaaaatcca gttccatacc gcacgggcaa cagagaccac cgtgtcacgg
      301 acggcacgca gaaccgtgcg tgcaacagag gcaacacaga cgctctccgc tgtgtcaaat
      361 atccggtcca ccgcaccggt aaactgttca cgggcctgag accggacaga ttccgtgcgc
      421 agctcgtcct gcaggcgggt catcagggga ctggcggcat ggtcgggaag cgctgtcatg
      481 agcgcactga ccagcgtatc cagttcccag ccggtgcggg ccgatacggc aacaaccgga
      541 tgtacgggcc ggaacagacg gaataccgcc tccgtttttt cgcgaatatt ctgtgcctgt
      601 gcgggagaag gctgaatgcc ggccatatcc cattcatggc agggctccgt tttgtcggcc
      661 tgcgtcacca caaacagtac ctgctgatgt ccccggtgca ggatgtgtcg ccagaaatac
      721 tcatccacag acagggcacg gtcatcggct ttaatcagcc acagtaccag gtcaagttca
      781 ggcagaatgt cacggtacag ggcttcatac tctgcatccc tgtcccggct ctcgcccacc
      841 ccgggcaggt cagtgataac catgctgtga ccatgtccac tcagacggaa gcgccgcact
      901 tcccgggtgc cggcgtgaac atcactgacc ggggtgacct ccccctgaaa cagtgcatta
      961 cagagtgagg atttaccggc cccgctttta cccataatgc caatcacggg ttcgtgactg
     1021 gtgagtttgc gcagatgttc caggatgtga cgggaaagtg agtaaggcag ggaggagagc
     1081 ggtttttcaa ttgcctcaat ggcatcagac ggattcatac atgtattccc ggtgaaaaaa
     1141 gacaaaaccc ccgcacaccg gaaaggtggc gggggactca cggagataat atctgtctga
     1201 aattattttg aatgtgccgg tcgcacagca aagaaggggc gatattctgc acatcctccc
     1261 ggttatgaat aacccggagt atctcaatgt ctaccgactt accccggggc tgtctgaacc
     1321 accggattac tgtagaattg cccctgatga ctcaggtctg aaccaggaaa aaacggataa
     1381 aaaatgacaa gtattcaaca acgagcagag ctgcaccgcc agatatggca aattgccaat
     1441 gatgtcagag gttctgttga cggatgggat tttaaacagt acgttctggg cgcacttttc
     1501 tatcgtttta tcagcgaaaa tttctccggg tatatggaag ccggtgatga cagcatctgt
     1561 tatgcgaaac tggatgacag cgtaattact gatgacatta aagacgatgc catcagaacc
     1621 aaaggttact tcatctaccc aagccagctg ttctgcaacg tggctgcgaa agcaaatacc
     1681 aatgacagac tgaatgcaga tttaaacagc atcttcgttg ctatcgaaag ttctgcttac
     1741 ggttatcctt cagaagctga catcaaaggt ttgtttgcgg atttcgatac caccagtaac
     1801 cgactgggta acaccgttaa ggataaaaat gcccgactgg ctgcggttct gaaaggggtt
     1861 gaaggattaa agcttggcga ctttaacaaa catcagattg acctgttcgg cgatgcctat
     1921 gagttcctga tttctaacta tgcagcaaat gccggtaagt caggcggaga attctttaca
     1981 ccgcagcacg tctctaagct gattgcacaa ctggctatgc acgggcagac ccacgttaac
     2041 aaaatctacg acccggcagc aggttccggt tcgctgttgt tgcaggctaa aaaacagttt
     2101 gataaccata tcattgaaga aggctttttc ggacaggaaa tcaaccatac gacctataat
     2161 ctggcgcgta tgaacatgtt tttgcacaac atcaactacg acaagtttga tatcaagctg
     2221 ggcaatacac tgactgagcc
//
LOCUS       DAZKFU020000427         2220 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZKFU020000427 DAZKFU020000000
VERSION     DAZKFU020000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.429"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            218..1831
                     /gene="tssA"
                     /locus_tag="ACHOQQ_RS24165"
                     /old_locus_tag="pgaptmp_004833"
     CDS             218..1831
                     /gene="tssA"
                     /locus_tag="ACHOQQ_RS24165"
                     /old_locus_tag="pgaptmp_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU4472968.1"
                     /translation="MSSPEALLTACAASQEERLSLISRANEALSLWDNWLHPFTSGSE
                     TGDDPAYDDNFQLMREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLTRD
                     GERGLSEGLLLLVAMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGVDSNEA
                     GLTAGAISLLGSAVADWPEAEKPSFAGLCSALENRLARSGGMEALVPQNSSPRETGRE
                     SAPPDSPQLAAVTSGRDLLDQARLLSRWLSGQPQGWLASHRLMKTVRWDTVDQLPSLD
                     SSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLWQGLS
                     HAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEEDVMY
                     GDEAAIPAVVNGQTDDVLILESEAMEKGDTEGPEAALAWLQSRPGMDTPRHRWLIRLL
                     MARVAEQYGRNDMALHLLGELITRAPQLTLEDWEPALLFEVRARRLKLLRMKAGRSES
                     DKARLMPEMDTLLAGLIAIDPARAMVLCG"
     gene            1865..>2220
                     /locus_tag="ACHOQQ_RS24170"
                     /old_locus_tag="pgaptmp_004834"
     CDS             1865..>2220
                     /locus_tag="ACHOQQ_RS24170"
                     /old_locus_tag="pgaptmp_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4472969.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     GVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYMRTIPSLAIVEFSPDWRSL
                     RQAEILAEGFSVLSRPV"
BASE COUNT          457 a          594 c          679 g          490 t
ORIGIN      
        1 caccctttca gagaacggct ccttcatgca tgcccgtcgg cgttttcgcc cacatgtcag
       61 aaaaaacaac cgataagctg acggaagggc aaacccactc agcaggagaa cgtatgctgc
      121 tgaaatagag gccaccacaa atcttcaaca gtaaggacga cgcttctccg gaagcggtcg
      181 tgaggtttat gaaagagact gataaacagg aaaaagaatg agttcaccgg aagccctgtt
      241 aacggcctgc gccgccagcc aggaagaaag gctgagcctc atttcccgcg caaatgaggc
      301 cctgtcgctc tgggataact ggctgcatcc atttacctcc ggatctgaaa ccggggacga
      361 tcctgcatac gatgataatt ttcagctgat gcgtgaagag atcaataaga tttcaggtac
      421 tgattcagcg ctgttgtgcg aactggctca gaagtgcctt tgcgaatgcg ccagagatat
      481 tcgtgtcgtg acctggtacg tgctggcccg tctcacccgg gatggtgaaa gagggttgtc
      541 cgaaggctta ttacttcttg ttgcgatgct gacccgctac ggacaggcat gtcatccccg
      601 gcgtcctgcc gcccggaaag ctgcgctgga gtggctgaac agtgcaaaag tgactgatgc
      661 actgctgtta tggcccgggg tggacagcaa tgaggccggt ctgacagcgg gtgccataag
      721 cctgctggga tccgccgtgg cagactggcc ggaagcggag aagccgtctt ttgcggggct
      781 ttgttccgcg cttgagaacc gcctggcacg ttcagggggc atggaggcac tggtccccca
      841 gaacagcagt ccccgggaga ccgggcgtga atcagctccc ccggactcac cacaactggc
      901 agcggtgacc tcggggcgcg acttactcga ccaggccagg ttactttccc gctggctgag
      961 cgggcagccg cagggctggc tggcttctca ccggctgatg aagacggtgc ggtgggatac
     1021 ggtcgatcag ctcccttcgc tggacagtag cggacgcacc cgcctggtcc ccccgaaaac
     1081 cgggtatcgc gctcagctta agcgactctg gcttcagaaa aactgggttg agctgattga
     1141 actggccagt cagatgtatt gcgaaggtgt gaatcatttc tggctcgatc ttcagtggta
     1201 tctctggcag ggactcagtc atgctggtca gccctgggat tcctggtctg acgctgtcct
     1261 gtcagatctg cggctgttgc ttaaacgtct cccgggcctt gaaggactgg catggaatga
     1321 cggtactccc tttgctgacg aggtcaccct cgcctggatc gctgaaaaag tgaatgaaga
     1381 agatgtgatg tacggcgatg aagctgccat tcctgccgtt gttaatggcc agactgacga
     1441 tgtgctgatc cttgagtctg aagccatgga aaagggagac acggaagggc ctgaagcggc
     1501 gcttgcctgg ctccagtccc ggccggggat ggatacacca cgtcatcgct ggctgatacg
     1561 cctgctcatg gcccgggtgg cagagcagta tggccggaac gatatggcgc ttcatctgct
     1621 tggtgaactt attacccgtg cgccacagct gacgctggaa gactgggagc ccgctttgct
     1681 gtttgaagtc cgggcccgac gcctgaagct gctacggatg aaagccggtc gcagcgaatc
     1741 tgataaagcc cgtctgatgc ctgagatgga tacgctgctg gcggggctga tcgccattga
     1801 cccggcccgt gccatggtgc tctgtggctg atgttctctt taatcatttt caggaatgcc
     1861 gtgcatggat gatttaaccc tgcgctatta cgaagcagag atgcgctatc tgcgggaagc
     1921 cggtaaagag tttgcccgtg ctcatccgga tcgggcagca atgctgaatc tggataagcc
     1981 tggcgcctgc gatcccggtg tggaacgtct gtttgaaggc tttgccttcc tgatgggacg
     2041 tttgcgtgaa aagctggatg atgacctgcc ggagctcacc gaagggctgg tgagtctgct
     2101 gtggccgcac tatatgcgga ctatcccgtc gctggccatc gttgagttct cgcctgactg
     2161 gcgtagtctg cgtcaggccg aaatactggc tgaaggcttc tccgttttgt cccgtcctgt
//
LOCUS       DAZKFU020000428         2215 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZKFU020000428 DAZKFU020000000
VERSION     DAZKFU020000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.430"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..216
                     /locus_tag="ACHOQQ_RS24175"
                     /old_locus_tag="pgaptmp_004835"
     CDS             <1..216
                     /locus_tag="ACHOQQ_RS24175"
                     /old_locus_tag="pgaptmp_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472970.1"
                     /translation="PLSENGSFTPVGVFAPMSEKTTGKRPEGQPHSAGESMLLKWRPP
                     QIVNSKDDASPEAVVRFMKETDKQEKE"
     gene            213..1826
                     /gene="tssA"
                     /locus_tag="ACHOQQ_RS24180"
                     /old_locus_tag="pgaptmp_004836"
     CDS             213..1826
                     /gene="tssA"
                     /locus_tag="ACHOQQ_RS24180"
                     /old_locus_tag="pgaptmp_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU4472971.1"
                     /translation="MSSPESLLTACAASQEERLSLISRANEALSLWDNWLHPFTSGSE
                     TGDDPAYDDNFQLMREEINKISGTDSALLCELAQKCLCECARDIRVVTWYVLARLIRD
                     GEKGLSEGLLLLVAMLTRYGQACHPRRPAARKAALEWLNSAKVTDALLLWPGVDSNEA
                     GLTAGAISLLGSAVADWPEAERPSFAGLCSALENRLARSGGMEALVPQNSSTRESGGE
                     SAPPDSPQLAAVTSGRDLLDQARLLSRWLNGQPQGWLASHRLMKTVRWDTVDQLPSLD
                     SSGRTRLVPPKTGYRAQLKRLWLQKNWVELIELASQMYCEGVNHFWLDLQWYLWQGLS
                     HAGQPWDSWSDAVLSDLRLLLKRLPGLEGLAWNDGTPFADEVTLAWIAEKVNEEDMMY
                     GDEAAIPAVVNDQTDDVLILESEAMEKGDTEGPEAALAWLQSRPGMDTPRHRWLIRLL
                     MARVAEQYGRNDMALHLLGELITRAPQLTLEDWEPALLFEVRARRLKLLRMKAGRSES
                     DKARLMPEMDTLLAGLIAIDPARAMVLCG"
     gene            1860..>2215
                     /locus_tag="ACHOQQ_RS24185"
                     /old_locus_tag="pgaptmp_004837"
     CDS             1860..>2215
                     /locus_tag="ACHOQQ_RS24185"
                     /old_locus_tag="pgaptmp_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4472972.1"
                     /translation="MDDLTLRYYEAEMRYLREAGKEFARAHPDRAAMLNLDKPGACDP
                     SVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYMRTIPSLAIVEFSPDWRSL
                     RQAEILAEGFSVLSRPV"
BASE COUNT          458 a          587 c          673 g          497 t
ORIGIN      
        1 cccctttcag agaacggttc cttcacgccc gtcggcgttt tcgcccccat gtcagaaaaa
       61 acaaccggta agcgaccgga agggcaaccc cactcagcag gagaaagtat gttgctgaaa
      121 tggaggccac cacaaatcgt caacagtaag gacgacgctt ctccggaagc ggtcgtgagg
      181 tttatgaaag agactgacaa acaggaaaaa gaatgagttc acctgaatcc ctgttaacgg
      241 cctgcgccgc cagccaggaa gaaaggctga gcctcatttc ccgcgcaaat gaggccctgt
      301 cgctctggga taactggctg catccgttca cctccggatc tgaaaccggg gacgatcctg
      361 catacgatga taattttcag ctgatgcgtg aagagatcaa taagatttca ggtaccgatt
      421 cagcgctgtt gtgcgaactg gctcagaagt gcctttgcga atgcgccaga gatattcgag
      481 tcgtgacctg gtacgtgctg gcccgtctta tccgcgacgg tgaaaaaggg ttgtccgaag
      541 gcttattact tcttgttgcg atgctgaccc gttacggaca ggcatgtcat ccccggcgtc
      601 ctgccgcccg gaaagctgcg ctggagtggc tgaacagtgc aaaagtgact gatgcactgc
      661 tgttatggcc cggggtggac agcaatgagg ccggtctgac ggcaggtgcc ataagcctgc
      721 tgggatccgc cgtggcagac tggccggaag ctgagaggcc gtcttttgcg gggctttgtt
      781 ctgcgcttga aaatcgtctg gcacgttcag gaggcatgga ggctctggtc ccccagaaca
      841 gcagcacccg ggagtccggg ggggaatcag ctcccccgga ctcaccacaa ctggcagcag
      901 tgacatcggg ccgcgactta ctcgaccagg ccaggttact ttcccgctgg ctgaacggac
      961 agccgcaggg ctggctggct tctcaccggc tgatgaagac ggtgcggtgg gatacggtcg
     1021 atcagctccc ttcgctggac agtagtgggc gtacccgcct ggtacccccg aaaaccggat
     1081 atcgcgctca gcttaagcga ctctggcttc agaaaaactg ggttgagctg attgaactgg
     1141 ccagtcagat gtattgcgaa ggggtgaatc atttctggct tgatcttcag tggtatctct
     1201 ggcagggact cagtcatgct ggtcagccct gggattcctg gtctgacgct gtcctgtcag
     1261 atctgcggct gttgcttaaa cgtctcccgg gccttgaagg gctggcatgg aatgacggta
     1321 ctccctttgc tgacgaggtc accctcgcct ggatcgctga aaaagtgaat gaagaagaca
     1381 tgatgtacgg cgatgaagct gccattcctg ccgttgttaa tgaccagact gacgatgtgc
     1441 tgatccttga gtctgaagcc atggaaaagg gagacacgga agggcctgaa gcggcgcttg
     1501 cctggctcca gtcccggccg gggatggata caccacgtca tcgctggctg atacgtctgc
     1561 tcatggcccg ggtggcagag cagtatggcc ggaacgatat ggcgcttcat ctgcttggtg
     1621 aactcattac ccgtgcgcca cagctgacgc tggaagactg ggagcctgct ttgttgtttg
     1681 aagtccgggc ccggcgtctg aagctgctac ggatgaaagc cggtcgcagc gaatctgata
     1741 aagcccgtct gatgcctgag atggatacgt tgctggcggg gctgatcgcc attgacccgg
     1801 cccgtgccat ggtgctctgt ggctgatgtt ctctttaatc attttcggga atgccgtgca
     1861 tggatgattt aaccctgcgc tattacgaag cagagatgcg ctatctgcgg gaagccggta
     1921 aagagtttgc ccgtgcccat cctgatcggg cagcaatgct gaatctggat aagcctggcg
     1981 cctgcgatcc cagtgtggaa cgtctgtttg aaggctttgc cttcctgatg ggacgtttgc
     2041 gtgaaaagct ggatgatgac ctgccggagc tcaccgaagg tctggtgagt ctgctgtggc
     2101 cgcactatat gcggactatc ccgtcgctgg ccatcgttga gttctcgcct gactggcgta
     2161 gtctgcgtca ggccgaaata ctggctgaag gcttctccgt tctgtcccgt cctgt
//
LOCUS       DAZKFU020000429         2199 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZKFU020000429 DAZKFU020000000
VERSION     DAZKFU020000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.431"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..630
                     /gene="aroL"
                     /locus_tag="ACHOQQ_RS24190"
                     /old_locus_tag="pgaptmp_004838"
     CDS             106..630
                     /gene="aroL"
                     /locus_tag="ACHOQQ_RS24190"
                     /old_locus_tag="pgaptmp_004838"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGU4472973.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            680..871
                     /gene="yaiA"
                     /locus_tag="ACHOQQ_RS24195"
                     /old_locus_tag="pgaptmp_004839"
     CDS             680..871
                     /gene="yaiA"
                     /locus_tag="ACHOQQ_RS24195"
                     /old_locus_tag="pgaptmp_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGU4472974.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            1129..1806
                     /locus_tag="ACHOQQ_RS24200"
                     /old_locus_tag="pgaptmp_004840"
     CDS             1129..1806
                     /locus_tag="ACHOQQ_RS24200"
                     /old_locus_tag="pgaptmp_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGU4472975.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            1878..2162
                     /gene="ppnP"
                     /locus_tag="ACHOQQ_RS24205"
                     /old_locus_tag="pgaptmp_004841"
     CDS             1878..2162
                     /gene="ppnP"
                     /locus_tag="ACHOQQ_RS24205"
                     /old_locus_tag="pgaptmp_004841"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGU4472976.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          585 a          498 c          553 g          563 t
ORIGIN      
        1 tggaattttt tctttacaat cgaaattgta ctagtttgat ggtatgatcg ctattctcat
       61 gacaccggct ttcgccgcat tgcgacctat tggggaaaac ccacgatgac acaacctctt
      121 tttctgatcg ggcctcgggg ctgtggtaaa acaacggtcg gaatggccct tgccgattcg
      181 cttaaccgtc ggtttgtcga taccgatcag tggttgcaat cacagctcaa tatgacggtc
      241 gcggagatcg tcgaaaggga agagtgggcg ggatttcgcg ccagagaaac ggcggcgctg
      301 gaagcggtaa ctgcgccatc caccgttatc gctacaggcg gcggcattat tctgacggaa
      361 tttaatcgtc acttcatgca aaataacggg atcgtggttt atttgtgtgc gccagtttca
      421 gtcctggtta accgactgca agctgcaccg gaagaagatt tacggccaac cttaacggga
      481 aaacctctga gcgaagaagt tcaggaagtg ctggaagaac gcgatgcgct atatcgcgaa
      541 gttgcgcata ttatcatcga cgcaacaaac gaacccagcc aggtgatttc tgaaattcgt
      601 agcgccctgg cacagacgat caattgttga ttttagagcg cctatactta acgttcatcc
      661 cgtgaaataa ggaagaacga tgccaacgaa accgccttat cctcgtgaag catatatagt
      721 gacgattgaa aaaggaaaac caggacagac ggtaacctgg taccaactca gagccgatca
      781 tcctaaacca gactcgttga tcagtgaaca tcctaccgct caggaagcga tggatgcgaa
      841 aaaacgctat gaggaccctg acaaagagtg accgcatcag actgctcgga agggattctg
      901 agtgccacta caagggatct gcgtcacatt tttcataatt catgtttttc aaataattag
      961 aatattaaac aataacaatc cattactgga atcatttgga atctttacat tatgccgtgc
     1021 acgtctgctg ctacgctttt tgtcatttgt agcacaagta agtgtcagca gtggtgctta
     1081 acacttgccc ggtaattaac gacgaaataa aagtaaggtg gaagaacaat gagtgcgtcg
     1141 ttggcgattc taaccatcgg cattgtacct atgcaggaag ttttgccgct cctgacagaa
     1201 tacattgacg aagataatat ttcccatcat agcctgctgg ggaagttaag ccgcgaagaa
     1261 gtgatggcgg agtacgcgcc agaagcaggc gaagacacaa ttctcacatt attaaatgac
     1321 aaccatctgg cccatgtttc gcgtcgcaaa gtagagcgtg acctgcaagg tgtggttgaa
     1381 gtgctcgata atcagggcta cgacgtcatt atattaatga gtacggcaaa cattagtagt
     1441 atgactgcgc gtaatacgat ctttcttgag ccgtcgcgga tattgccacc gctggtttcc
     1501 tctattgttg aagatcatca ggtgggggtt atcgtaccgg ttgaggagtt gctgaccgtt
     1561 caggcgcaaa aatggcaaat tttgcagaaa ccgccggtat tttcattggg taaccccatt
     1621 catgattcag aacaaaaaat cattgatgcc gggaaagaat tactggcgaa aggcgcagat
     1681 gtcatcatgc tggattgttt gggatttaac cagcgtcatc gcgatttact gcaaaaacag
     1741 ctcgatgttc ctgtcttgct ctctaacgta ttgattgcac ggttggctgc ggaattactg
     1801 gtgtaatttt gcgtgacagc cagcgcctct ggcccctata gtgaagtaga tgttcaacta
     1861 ccagacaggg ccagtttatg cttcaaagta atgagtactt ttccggcaaa gtgaaatcga
     1921 tcggcttttc cagcagcagc actggtcgcg ccagcgtggg tgttatggtt gaaggcgaat
     1981 acaccttcag caccgctgag ccggaagaga tgacggtaat cagtggcgcg ctgaatgtgt
     2041 tactgcctga cgcgaccgac tggcaagtgt atgaagccgg ttcggtgttt aatgttcccg
     2101 gtcacagtga gtttcatctg caagttgccg aacccacctc ttatctgtgc cgctatctgt
     2161 aattcctcgc cttccccttg aacgggaggg catttttct
//
LOCUS       DAZKFU020000430         2197 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZKFU020000430 DAZKFU020000000
VERSION     DAZKFU020000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.432"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..345)
                     /gene="hiuH"
                     /locus_tag="ACHOQQ_RS24210"
                     /old_locus_tag="pgaptmp_004842"
     CDS             complement(<1..345)
                     /gene="hiuH"
                     /locus_tag="ACHOQQ_RS24210"
                     /old_locus_tag="pgaptmp_004842"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGU4472977.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINK"
     gene            478..1149
                     /gene="hprR"
                     /locus_tag="ACHOQQ_RS24215"
                     /old_locus_tag="pgaptmp_004843"
     CDS             478..1149
                     /gene="hprR"
                     /locus_tag="ACHOQQ_RS24215"
                     /old_locus_tag="pgaptmp_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGU4472978.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1149..>2197
                     /gene="hprS"
                     /locus_tag="ACHOQQ_RS24220"
                     /old_locus_tag="pgaptmp_004844"
     CDS             1149..>2197
                     /gene="hprS"
                     /locus_tag="ACHOQQ_RS24220"
                     /old_locus_tag="pgaptmp_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGU4472979.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEVCF
                     NVECNQQIFADKILL"
BASE COUNT          644 a          397 c          508 g          648 t
ORIGIN      
        1 tttattaata tgaaattcaa cggggatctc agggaagaaa ctttcaagat tttgtttctt
       61 gaaatagtcc ccggttttaa atacgacacg gtaatcgccc gtagttgcag tttgctcggg
      121 ccacagtgcc ttaattcgtc catccttatc tgttttggca gtattaagtt gtaaccagcc
      181 gttgtccgct ttcttttcaa gagtgactgt cacgtcggca gcgggttttc ctgtttgctg
      241 gttcaaaatg tgcacgctaa gaatgttttg ttgtgcagca tagaccaaag aaggtaatga
      301 aaatgcagcc gttactacgg agagtactaa ataacgcttt aacatgttta tatccttgtc
      361 atgtgaatga gttttgagga tagcgaaaaa gcgtccaggg taaccttaca gcagcattac
      421 aattttgtaa tgaaccaggc tgtttctata acatatgatt tatggcatat tattttcatg
      481 aagattttac ttattgaaga taatcaaagg acccaagagt gggtaacgca ggggctttcc
      541 gaagcgggtt atgtcattga tgccgtttct gatggcagag atgggcttta tcttgcgctg
      601 aaggatgatt atgcattgat cattctggat attatgcttc cgggtatgga tggctggcag
      661 atcttacaaa cgctaagaac agcaaagcaa acccctgtta tttgccttac tgcaagggat
      721 tctgtcgatg acagagtcag agggctggac agtggggcaa atgattatct ggtaaaacct
      781 ttttcatttt ctgagttgct ggcaagggtt cgggcacaat taagacaaca tcacgctttg
      841 aattcaacat tagaaatcag cggcttaaga atggactctg ttagtcaaag tgtgagcagg
      901 gacaatatca gtattacact gacgcgcaag gagtttcagt tactttggct gctggcctcc
      961 agagctggcg agattatccc cagaacggtt attgcgagtg aaatttgggg aatcaacttt
     1021 gatagtgata ccaatacggt ggatgtcgcc attcgcaggc tccgcgcaaa agttgatgat
     1081 ccttttcctg aaaagctaat tgccacaatc cgtgggatgg gatattcgtt cgtagcggta
     1141 aaaaaataat gaaaagatta tctataaccg tccgtttaac cttgcttttt atattgctgc
     1201 tgtctgttgc tggcgccgga attgtctgga ctctctataa tggcctggca agtgagttga
     1261 aatggcgcga tgatacaaca ctcattaacc ggacagcaca gatcaagcag ttgttaattg
     1321 atggggtaaa tccagatacg ttacctgtat actttaaccg gatgatggat gttagtcagg
     1381 atatcttgat cattcatggt gatggcatca ataaaattgt taaccggaca aatgtcagtg
     1441 atgacatgtt aaataacata cctgctagtg agacaatcag cgcagctggc atttacagaa
     1501 gcattattaa tgacactgaa attgatgctt tacgaattaa tattgatgaa gtttcgccat
     1561 cattaacggt tactgtggct aaattggctt cagccagaca taacatgctt gaacagtata
     1621 aaattaatag cattataatt tgcattgtcg ccattgtact ttgctcagta ttaagtccgc
     1681 tgttaatcag aacgggatta cgagagatca aaaagttgag tggtgtaacg gaagcgctga
     1741 attataacga tagccgggag cctgttgagg ttagcgcatt accgagagaa ctaaaacctc
     1801 ttgggcaggc gttgaataaa atgcatcatg ctttagtcaa agattttgag cgtctaagtc
     1861 agtttgctga cgatctcgct catgaactta gaacgccaat taatgcatta ctgggtcaga
     1921 atcaggttac gctaagtcaa accagaagta tcgctgaata tcaaaaaaca attgccggaa
     1981 acattgaaga gctggaaaat atttcgcggt taacagagaa catactgttt cttgccaggg
     2041 cagataaaaa caatgttttg gtgaaactgg actcgctttc tctcaataag gaagtcgaaa
     2101 atttgttgga ctatcttgaa tatctttcag acgagaaaga ggtttgcttt aatgtcgagt
     2161 gcaatcagca aatctttgcg gataaaattt tactgca
//
LOCUS       DAZKFU020000431         2184 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZKFU020000431 DAZKFU020000000
VERSION     DAZKFU020000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.433"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            201..383
                     /locus_tag="ACHOQQ_RS24225"
                     /old_locus_tag="pgaptmp_004845"
     CDS             201..383
                     /locus_tag="ACHOQQ_RS24225"
                     /old_locus_tag="pgaptmp_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4472980.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            409..681
                     /locus_tag="ACHOQQ_RS24230"
                     /old_locus_tag="pgaptmp_004846"
     CDS             409..681
                     /locus_tag="ACHOQQ_RS24230"
                     /old_locus_tag="pgaptmp_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4472981.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEACLDAEVDA
                     ILDELLGVPAEPPTEAGDTTAESGELQPESPVSDATEPQPESVMML"
     gene            757..972
                     /locus_tag="ACHOQQ_RS24235"
                     /old_locus_tag="pgaptmp_004847"
     CDS             757..972
                     /locus_tag="ACHOQQ_RS24235"
                     /old_locus_tag="pgaptmp_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4472982.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            976..1533
                     /locus_tag="ACHOQQ_RS24240"
                     /old_locus_tag="pgaptmp_004848"
     CDS             976..1533
                     /locus_tag="ACHOQQ_RS24240"
                     /old_locus_tag="pgaptmp_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472983.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSTLMPSEDEKKRRDEQFAAF
                     DDYCRKVMSRNLAECFSIHNDNFSELEWECNRPSFVVSGDGGKITISGNGKVTPPSHQ
                     HSEELIEFAIDYLKNNKKQGLMQRIGRCMGYLQVAAEIEALASGADKDSVVREALLRD
                     FDNPPFKKVPAYWLHPGLTYLKVRI"
     gene            1570..2103
                     /locus_tag="ACHOQQ_RS24245"
                     /old_locus_tag="pgaptmp_004849"
     CDS             1570..2103
                     /locus_tag="ACHOQQ_RS24245"
                     /old_locus_tag="pgaptmp_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4472984.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGNPVIPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            2100..>2184
                     /locus_tag="ACHOQQ_RS24250"
                     /old_locus_tag="pgaptmp_004850"
     CDS             2100..>2184
                     /locus_tag="ACHOQQ_RS24250"
                     /old_locus_tag="pgaptmp_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4472985.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRD"
BASE COUNT          573 a          474 c          626 g          511 t
ORIGIN      
        1 catatacgct gctgattgac agtgtgaagg tggaagtgaa caacgcggac gtcgcggcat
       61 gataaaaaaa gccgccagcg gcaggaatgg aagctggcgg aggtaatccc aatggagaat
      121 gtaaagaaaa gatgctttcg tacatcggtt gtttttttaa tgaaaacagt tctcattgtc
      181 aaccataacg gtaagaaatt atgacattta ttcatcaggt gatgttgtac ttctgtacgg
      241 cggtctgtgt gctgtatctc ctttcgggtg ggtacagggc agtgcgcgat ttctggcgca
      301 ggcagattga taaaagggcc gcagagaaaa tcagcgccag tcagtcagcc ggaagcaaac
      361 ccgaagagcc cgttactcct taacaacccc tttcagcgag aaaatcccat gtcagaaatc
      421 acatccctgg tcactgcaga ggcagtgaag gaagtcctgc gctctgaaga agtcctgagc
      481 gcactgaaac agaaactccg ccagaacctt gaggcgtgtc ttgatgcaga agttgatgcc
      541 attctggatg agctgcttgg cgtaccggca gagccaccga ctgaagcggg agataccacc
      601 gcagagagcg gtgaacttca gcctgaatca ccggtctccg atgcgactga accgcaaccc
      661 gaatcggtca tgatgctgta acggggtgtc ggggccatca gtaaactgct gctggccttt
      721 ttcatgttgt gagcttccgt gtgcgggaga cggggtatgt accagatgga aaaaatcaca
      781 acaggtgtgt catacaccac gtcagcggtg ggaacgggct actggttcct gcagttgctg
      841 gacagggttt ccccgtctca gtgggcggca ataggtgtgc tggggagtct gctgtttggg
      901 ctgctgacgt acctgacgaa cctgtatttc aaaattaaag aagaccggcg taaggccgcc
      961 cggggagagt gaataatgaa ccatgaagaa atgaatcagc gcttcagtcg cctggaaaat
     1021 gaaattgctg aactgaataa aaaactgtcg acgctgatgc cttctgaaga tgaaaaaaaa
     1081 cgccgcgatg agcagtttgc tgcgtttgac gattattgtc ggaaagtgat gagcagaaat
     1141 ctcgcagagt gtttcagtat tcataatgat aatttcagtg agctggaatg ggagtgtaac
     1201 cggccatcct ttgttgtatc cggtgatggt gggaaaataa ccatctcagg aaatgggaaa
     1261 gtaacacctc catcgcacca gcacagtgag gagctcattg aatttgccat tgattacctg
     1321 aagaacaata aaaaacaggg gctgatgcag cgcattggtc gttgcatggg gtatctgcag
     1381 gtagccgctg agattgaagc gctggccagt ggtgcggaca aggattcagt tgtgcgggag
     1441 gcacttcttc gtgattttga taatccgccc tttaaaaaag tgccggctta ctggcttcat
     1501 cccggactga cttatctgaa agtgcgtatt tagtgggcct gggacagcgg ctgaatattt
     1561 aatatatcca tgaacacaaa aataaaatac ggcctgtcgg ctgccgttct ggcgttgatt
     1621 gccgcagggg cttctgcgcc tgaaatcctc gaccagtttc tggatgaaaa ggaaggcaac
     1681 cacaccacgg cataccgtga tggtgcgggt atctggacca tctgccgtgg agccacccgg
     1741 gtggatggta atcctgtgat tcctggcatg aagctgtcga aggaaaaatg cgaccaggtt
     1801 aacgccattg aacgggataa ggcgctggca tgggtggaga aaaacatcag agtgccactg
     1861 actgaacccc agaaagcggg gatcgcgtca ttctgtcctt acaacattgg cccgggtaag
     1921 tgtttcccgt cgacgtttta taaacgaatt aatgcaggcg atcgcagggg agcgtgtgaa
     1981 gcgattcgct ggtggattaa ggacggtggg cgtgattgcc gcacacgttc aaataactgc
     2041 tacggacagg ttattcgtcg tgaccaggaa agcgcattag cttgttgggg gatagatcag
     2101 tgagaagagt aaccgcgatt atttacgctc tggttatctg catcatcgtc tgtctgttat
     2161 gggctgttaa tcattaccgc gata
//
LOCUS       DAZKFU020000432         2181 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZKFU020000432 DAZKFU020000000
VERSION     DAZKFU020000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.434"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..678
                     /locus_tag="ACHOQQ_RS24255"
                     /old_locus_tag="pgaptmp_004851"
     CDS             <1..678
                     /locus_tag="ACHOQQ_RS24255"
                     /old_locus_tag="pgaptmp_004851"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4472986.1"
                     /translation="PPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVG
                     RAVVKLPLMGKSLNKSIERNKVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRG
                     LIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLN
                     VDLEVACMRKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDF
                     MTARGYH"
     gene            complement(727..>2181)
                     /gene="pitB"
                     /locus_tag="ACHOQQ_RS24260"
                     /old_locus_tag="pgaptmp_004852"
     CDS             complement(727..>2181)
                     /gene="pitB"
                     /locus_tag="ACHOQQ_RS24260"
                     /old_locus_tag="pgaptmp_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HGU4472987.1"
                     /translation="LLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVVMAAF
                     FNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLP
                     ASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLIVSPIVGLVIAGGLIF
                     LLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKG
                     IGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLITMEPPL
                     PAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSVSQRSQLRRIMLCISD
                     TSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVAM
                     TIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGTMVVDGGGL
                     QRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          570 a          577 c          542 g          492 t
ORIGIN      
        1 cctccattaa tagccgttat tggtagcgat ggttcaggca agtcaacggt gtgtgaacat
       61 ctcattaccg ttgtcgaaaa atatggcgcc gcagaaagag ttcatctcgg aaaacaagcc
      121 ggaaatgtcg gtcgtgcagt ggtaaaactg cccttaatgg gaaaatcgct taataaatct
      181 attgaacgca ataaagtgaa aacagcaaaa aaattgccag gaccagttcc ggcgctggta
      241 attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt
      301 cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg cgcttacgat
      361 ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct ggcaagccag
      421 gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat caaactcaat
      481 gttgaccttg aagttgcctg catgcgtaaa cccgaccata aacgggaatc gctggcgagg
      541 aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga tatcgatgcc
      601 aatcagccac tggaacaggt gctggttgat gcagaaaaag cgattacgga ttttatgacc
      661 gcgcgtggtt atcactagcc aaaaatggaa atgcccgatc gccaggaccg ggcattttca
      721 ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa aaatcgccgc
      781 cggtaaagta aatacccacg ccatcaggat gctggttacc gttttacgct gtaacccacc
      841 gccgtccacc accatcgtcc ctgcaactgc agacgagagg acgtgtgttg tggagacggg
      901 catcccaata taactggcaa gaccgataga cactgccgcc gtcatttgtg ccgccatgcc
      961 ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg ctacacgacg
     1021 ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga tccacaccgg
     1081 agcgtactca atggtgctta acatatcgct gcgaagtttt ttcagcaggt tctggtcttc
     1141 tttactgacg cctggcagtt tcgccagctt cgcggaggta tcagagatgc acagcataat
     1201 gcggcgcaac tggctgcgct gactcacgct taacggctcg taactttcca tattgcctgg
     1261 cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac agtgaaactc
     1321 cgtcacttgc gcgccatcag tagatgccgc aggcaatgga ggttccatcg taatcaactt
     1381 ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtgacgg catcgcgggt
     1441 acgggtaatt tcatagccgg acgcgttcat attgacgacg aagccagcag gggcaatccc
     1501 caccagtacc agcatcacca ggccgatccc tttctgcccg tcgttagcgc cgtgtgaaaa
     1561 cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg gcttacgttt
     1621 gccttttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct ttttcgttcc
     1681 gctccagtag cgtcgcagca ggaatatcag gcctcccgca atgaccaggc cgacgatagg
     1741 ggaaacaatc agcgaggaga aaattttggt cacttcacgc aggtttaacg catccatcac
     1801 cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcgc caatcaaggt
     1861 atgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga taatcgccgc
     1921 tagcagcatg gaaaagacca tcgccagacc gtgggttgac cccatattca gcaacaaatc
     1981 ggttggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata acacgccaaa
     2041 aaagttaaaa aatgccgcca tcaccacagc aagttgtggt tgcatggcac gagtataaat
     2101 aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt agaacaacac
     2161 aaatgccaga gcaagtaata a
//
LOCUS       DAZKFU020000433         2175 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZKFU020000433 DAZKFU020000000
VERSION     DAZKFU020000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.435"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..313
                     /gene="essQ"
                     /locus_tag="ACHOQQ_RS24265"
                     /old_locus_tag="pgaptmp_004853"
     CDS             98..313
                     /gene="essQ"
                     /locus_tag="ACHOQQ_RS24265"
                     /old_locus_tag="pgaptmp_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4472988.1"
                     /translation="MKSMDKLTTGIAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKVARRE"
     gene            318..653
                     /locus_tag="ACHOQQ_RS24270"
                     /old_locus_tag="pgaptmp_004854"
                     /pseudo
     CDS             318..653
                     /locus_tag="ACHOQQ_RS24270"
                     /old_locus_tag="pgaptmp_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(817..1077)
                     /locus_tag="ACHOQQ_RS24275"
                     /old_locus_tag="pgaptmp_004855"
     CDS             complement(817..1077)
                     /locus_tag="ACHOQQ_RS24275"
                     /old_locus_tag="pgaptmp_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472989.1"
                     /translation="MDYTNLPKQTFADLIALRQAVVALINLLPEKEKELVKALLNRTA
                     ADFSSYPLTDDLADLPELIAASAIKLTEEIYPPQKSSQNSCE"
     gene            1191..1724
                     /locus_tag="ACHOQQ_RS24280"
                     /old_locus_tag="pgaptmp_004856"
     CDS             1191..1724
                     /locus_tag="ACHOQQ_RS24280"
                     /old_locus_tag="pgaptmp_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4472990.1"
                     /translation="MNVKIRYSLSAAVLALIAASAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCAQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEH"
     gene            1721..>2175
                     /locus_tag="ACHOQQ_RS24285"
                     /old_locus_tag="pgaptmp_004857"
     CDS             1721..>2175
                     /locus_tag="ACHOQQ_RS24285"
                     /old_locus_tag="pgaptmp_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HGU4472991.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRTISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGVTC"
BASE COUNT          601 a          449 c          578 g          547 t
ORIGIN      
        1 ttagcgccgt tgagaacaac gtttgctata aaaactaacc catagactcc gatcttttca
       61 aacatattgc accatctgtg tacatcgggg tgaggatatg aaatcaatgg ataagttaac
      121 aacaggtatc gcctatggca catcggctgg taatgctggt ttctgggcat tgcagttact
      181 cgataaagta actccgtcac agtgggctgc aatcggtgtg ctgggtagtc tggtttttgg
      241 cctgctgacg tatctgacaa acctttattt caagattaaa gaagacaggc gtaaggttgc
      301 gagaagagag taatccaatg acacaagact atgaactggt tgtgaaagga gtccgcaatt
      361 ttgagaataa agtggcggta actgtagcct tacgggacaa aaaacgcttt gacggtgaaa
      421 tttttgacct ggacatctcg ctggaccgtg ttgaaggtgc cgcgctggag ttttatgagg
      481 cagcagccag aatgagaatc agacaggtat tcctggatgt tgctgccggg ttatgtgtag
      541 gggatgagca gtcgccggaa aagcgcccca taattttaga ggcgcagaat gtgtggataa
      601 cctacaaagg aaagctaccg ggaagaatta ctggttctct gaagacgcca ccgacggcat
      661 tgcggtcaga aaaagatgat attgaatcgc ccattgaaaa actggagggc agcgtcgctg
      721 atctgaataa aaaattgtcg gtgctgatcc cttctgaaga tgaaaaaaaa cgccgcgatg
      781 agcagtttgc ggcgttttac gattattgca ttgaagttac tcgcaggaat tttgtgaaga
      841 tttttgagga gggtaaatct cttcagtaag cttaatggcg gacgctgcaa ttaattcagg
      901 aaggtccgca aggtcatctg tcagtggata tgatgaaaaa tcggcggcag ttctgttaag
      961 aagcgcttta actaattcct tttccttctc cggcaacaag ttgattagag ctacgactgc
     1021 ttgcctgagt gcgattaaat cagcaaaagt ttgttttggt agatttgtgt aatccatagt
     1081 cacctctgtg tttatcagat tgacatcctc ctcccgccag tgcccatcac tggtgaggta
     1141 agatttaaca tatccgggga tttgaagccg ataaatcctg ataaatatcc atgaacgtaa
     1201 aaatcagata cagcctgtcg gctgctgttc tggcactgat tgccgccagt gctcctgccc
     1261 ctgacattct cgatcagttt ctggatgaaa aagaggggaa tcacaccact gcataccgcg
     1321 atggctccgg catatggacc atctgtcggg gagcaacgat ggtggacggt aaacctgtta
     1381 ttccgggaat gaaactgtcg aaggaaaaat gcgcccaggt taacgccatt gagcgggata
     1441 aggcgctggc atgggtggag cgcaatataa aagttccact gaccgagcca cagaaagccg
     1501 gtatcgcgtc attctgtccc tataacattg gccctggtaa gtgttttccg tcgacgtttt
     1561 ataagcggct taatgccggt gatcgtaaag gcgcatgcga ggcgattcgc tggtggataa
     1621 aggacggtgg acgtgattgc cgcattcgtt caaataattg ttacggtcag gttattcgtc
     1681 gtgaccagga gagtgcatta acctgctggg ggatagaaca ttgaatcaga tattcacggt
     1741 gattttgctc gtgttggtag gatttgtcgt aggtaatgtc tggagcgaca gaggatggca
     1801 aaaaaaatgg gcggagcgtg atgctgccga attatctcaa gaggtaaatg tccaatttgc
     1861 tgctcgaata attgaacagg ggcgaactat atcccgtgat gaggctgtta aagatgcaca
     1921 acagaaagcc gctgaaattt ctgccagggc tgctgatctg tctgatagtg ttaaccagct
     1981 gcgtgccgaa gcaacaaaat atgccatacg ccttgacgca gcgcagcata ccgcaaatct
     2041 tgccgctgcc gtcagaggca aaacaaccaa agccgccgaa ggaatgctca ccaacatgct
     2101 cggagatatt gcagcagaag ctcagcttta tgctgaaatt gctgacaaac gctacatcgc
     2161 aggagtgact tgtca
//
LOCUS       DAZKFU020000434         2155 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZKFU020000434 DAZKFU020000000
VERSION     DAZKFU020000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.436"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..531
                     /locus_tag="ACHOQQ_RS24290"
                     /old_locus_tag="pgaptmp_004858"
     CDS             22..531
                     /locus_tag="ACHOQQ_RS24290"
                     /old_locus_tag="pgaptmp_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4472992.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            503..>2155
                     /locus_tag="ACHOQQ_RS24295"
                     /old_locus_tag="pgaptmp_004859"
     CDS             503..>2155
                     /locus_tag="ACHOQQ_RS24295"
                     /old_locus_tag="pgaptmp_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4472993.1"
                     /translation="MNISEQQLNNMMSSVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVAGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVESTIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPE"
BASE COUNT          548 a          493 c          600 g          514 t
ORIGIN      
        1 agagtttttg cgaggtgagg agtggctacg cagactgaag ttgccaggca tttaagtctg
       61 accgatcgcc agcttcgcag attgcagaaa ttgccgggtg ccccgatatc gaataagcga
      121 gggcaactgg atctggatgc ctggcgtgat ttttacatat cgtatctgag gagaagtaaa
      181 aacgatgtgc ctgatggcga tagcgaagac gactatgagg agaaattgct tattgccaga
      241 tgggaactga cagcggaaca ggctgttaca cagcagttaa aaaatgaggt gtcaaaagga
      301 aaacttattg acaccgggtt ctgtattttt gccctcagta agctggcaat ggcgttatcc
      361 agtacgcttg attccatccc tttatccatg cagcgacagt ttcctgattt aacaccgcgc
      421 catcttgacc atctgaaaac ccttattgcg aagggggcaa atcagtgtgc gcgggcgggg
      481 gataaattac cggatttact cgatgaatat atcagagcaa caactgaata atatgatgag
      541 ctctgtcaca acagcattac agcccctgat aagggcattg ccggtgacgc cagttgaatg
      601 ggctgatcaa aattattatc tgcctaaaga atcttcatat ggtgagggag aatggaaaac
      661 gctgccgttc cagatcgcca tcatgaacag tatggggaat gatcagatcc gcactgttaa
      721 tctgattaaa tctgcccgtg ttggctatac aaagatgttg ctgggagtcg ccgggtattt
      781 tattgagcat aaatcccgaa acagtctgct ttttcagcct acggattctg ccgctgaaga
      841 ttttatgaaa tctcacgtgg aatccacgat tcgcgatgtt ccctgcctga aaaaactttc
      901 tccctggctg ggacgtaaac atcgtgataa taccctcacg ctgaaacgct tttcatcggg
      961 tgtgggtttc tggtgcctgg gtggtgcggc agcaaaaaac taccgtgaaa aatctgtgga
     1021 tgtggtctgc tatgacgaac tttcctcgtt cgagccggat gttgaaaaag agggctcgcc
     1081 aaccctgctg ggggataaac gtattgaggg ctcggtatgg ccaaaatcca ttcgcggctc
     1141 gacgcctaaa ataaaaggtt cctgccagat cgaaaaagcg gccaacgagt cggcgcattt
     1201 catgcgtttt tatgtgccct gcccgcactg tggggaggcg cagtatctga aatttggcga
     1261 tgagtccacg ccttttggcc ttaaatggga gaaggacagc cccgaaagcg ttttctacct
     1321 ctgtgaacat catggctgcg tgatccatca gtctgagctt gaccagagca acgggcggtg
     1381 gatctgtgaa aacacgggga tgtggacccg tgacggtctg acgtttttca gcgctgcggg
     1441 taatgaaatt ccaccgccgc gctccatcac attccatatc tggacggcgt acagtccgtt
     1501 caccacctgg gtacagatag tctatgactg gctggatgca ctgaaagatc ccaacggcct
     1561 gaaaactttt gtgaacacca cgctgggcga gacctgggaa gaggccgtgg gcgaaaaact
     1621 cgatcaccag gtactgatgg ataaggttgt gcgttacacg gcggcggtgc ctgcccgggt
     1681 ggtttatctg acggcgggca ttgactcgca gcgaaaccgt tttgagatgt atgtctgggg
     1741 atgggctccg ggagaggaag cctttctggt ggataaaatc atcattatgg gacgtccaga
     1801 tgaggaagag acgctgttac gtgtggatgc ggcgatcaac aaaaaatatc gccatgcgga
     1861 tggcaccgaa atgaccattt cccgtgtctg ctgggacacc ggggggatcg atggcgaaat
     1921 cgtttatcag agatcaaaaa aacacggtgt tttccgtgtg ctgccggtaa aaggcgcgtc
     1981 tgtctatggc aagccggtga tcaccatgcc aaaaacccgc aatcagcggg gcgtgtatct
     2041 gtgtgaagtg gggacggaca ccgcaaaaga aattctctat gcccgtatga aagccgatcc
     2101 cacgcctgcg gatgaagcca cgtcgtatgc catccgtttt cctgatgatc cggag
//
LOCUS       DAZKFU020000435         2147 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZKFU020000435 DAZKFU020000000
VERSION     DAZKFU020000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.437"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..2115
                     /gene="yjcS"
                     /locus_tag="ACHOQQ_RS24300"
                     /old_locus_tag="pgaptmp_004860"
     CDS             130..2115
                     /gene="yjcS"
                     /locus_tag="ACHOQQ_RS24300"
                     /old_locus_tag="pgaptmp_004860"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGU4472994.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSEKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          568 a          563 c          536 g          480 t
ORIGIN      
        1 aaatggttcg ttccgctcac acttccactt attaaagtcg caatttccca attatgataa
       61 cgccagcttt aaatgacgta ttttgcatgg attacatatt tacgacataa atgataaatg
      121 gagatttata tgaataactc tcggttattc cgtttgagca ggattgttat tgcgttaact
      181 gccgccagcg gcatgatggt aaataccgct aacgcgaaag aggaagcgaa agccgccact
      241 caatataccc aacaggttaa tcagaattac gccaaatcat taccgtttag cgatcgtcag
      301 gattttgacg atgcccagcg tggatttatc gccccgctgc tggatgaagg tattctgcgt
      361 gatgcgaacg gtaaagttta ctaccgcgcg gacgattaca aatttgatat taatgccgca
      421 gcgccggaaa ccgtaaaccc cagcctgtgg cgtcagtcgc aaatcaacgg tatttctggc
      481 ctgttcaaag tcaccgataa aatgtatcag gtgcgcggcc aggatatctc taacattacg
      541 ttcgttgagg gcgagaaagg cattattgtt atcgacccgc tggtgacgcc gcctgccgca
      601 aaagccgcac ttgaccttta cttccagcat cgtccgcaaa aaccgattgt tgccgttatc
      661 tacactcaca gccacaccga ccactatggt ggcgtgaaag gcattatctc tgaagccgat
      721 gttaaatccg gcaaagttca ggtgattgcc cctgctggct ttatggacga agccatcagc
      781 gaaaacgtgc tggcgggtaa catcatgagc cgccgtgcgc tctactctta cggtctgtta
      841 ctgccgcaca acgcgcaagg caacgtgggt aatggccttg gcgtgacgct ggcaacgggc
      901 gacccgagca ttattgcacc gactaaaact atcgtccgaa ctggcgagaa gatgattatc
      961 gacggcctgg agtttgactt cctgatgacc ccaggtagcg aagcaccagc cgaaatgcac
     1021 ttctatattc cggccctgaa agccctgtgt accgccgaga acgccacgca taccctgcac
     1081 aacttctaca ctctgcgcgg cgcgaaaacc cgcgatacca gcaagtggac cgagtacctg
     1141 aacgaaacgc tggatatgtg gggtaacgac gcggaagtac tgtttatgcc gcacacatgg
     1201 ccggtctggg gcaataagca tatcaatgat tatattggta aatatcgcga tactatcaag
     1261 tacattcacg accagaccct gcacctggcg aaccagggct acaccatgaa tgaaatcggc
     1321 gacatgatta agctgccgcc tgcacttgcc aataactggg ccagccgtgg ctattacggt
     1381 tctgtcagcc acaacgcccg cgcggtgtat aacttctatc ttggctatta cgacggtaac
     1441 ccggctaacc tgcatccgta tggtcaggta gagatgggca aacgttacgt gaaagcgctg
     1501 ggcggttctg cccgtgtcat caacctggcg caagaagcga acaagcaagg tgattaccgc
     1561 tggtcggcag aactgctgaa acaggtgatt gccgccaacc cgggtgacca ggtcgcgaag
     1621 aatctgcaag cgaataactt tgaacagctg ggctatcagg ccgagtccgc cacatggcgc
     1681 ggtttctacc tgaccggcgc gaaagagctg cgcgaagggg tgcataagtt cagccacggc
     1741 accaccggtt ccccggacac cattcgcgga atgtcggtcg aaatgctgtt cgactttatg
     1801 tccgttcgcc tcgatagcga aaaagccgcg ggtaaaaata tcagcctgaa cttcaatatg
     1861 agcaacggcg ataacctcaa cctgacgctg aacgatagcg tgcttaacta ccgtaaaacg
     1921 ctgcaatccc aagctgacgc ctctttctac atcagtcgtg aagatctgca cgccgtgctg
     1981 accggacaag ccaaaatggc ggatctggta aaagcgaaga aagccaaaat tattggcaat
     2041 ggtgcgaaac tggaagaaat tatcgcctgt ctggataatt tcgatttgtg ggtgaatatc
     2101 gtaaccccaa attaatgcca ctcacttttg ggggcgctta agccccc
//
LOCUS       DAZKFU020000436         2141 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZKFU020000436 DAZKFU020000000
VERSION     DAZKFU020000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.438"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(61..273)
                     /locus_tag="ACHOQQ_RS24305"
                     /old_locus_tag="pgaptmp_004861"
                     /pseudo
     CDS             complement(61..273)
                     /locus_tag="ACHOQQ_RS24305"
                     /old_locus_tag="pgaptmp_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            423..1091
                     /locus_tag="ACHOQQ_RS24310"
                     /old_locus_tag="pgaptmp_004862"
     CDS             423..1091
                     /locus_tag="ACHOQQ_RS24310"
                     /old_locus_tag="pgaptmp_004862"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4472995.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1036..1173)
                     /locus_tag="ACHOQQ_RS24315"
                     /old_locus_tag="pgaptmp_004863"
     CDS             complement(1036..1173)
                     /locus_tag="ACHOQQ_RS24315"
                     /old_locus_tag="pgaptmp_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HGU4472996.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1146..1730)
                     /locus_tag="ACHOQQ_RS24320"
                     /old_locus_tag="pgaptmp_004864"
     CDS             complement(1146..1730)
                     /locus_tag="ACHOQQ_RS24320"
                     /old_locus_tag="pgaptmp_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4472997.1"
                     /translation="MAFRMSEQPRTIKIYNLLSGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(1730..>2141)
                     /locus_tag="ACHOQQ_RS24325"
                     /old_locus_tag="pgaptmp_004865"
     CDS             complement(1730..>2141)
                     /locus_tag="ACHOQQ_RS24325"
                     /old_locus_tag="pgaptmp_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4472998.1"
                     /translation="SSTDLGTKTTSSFDYGTKSTNNIGAHTHSVSGTAASAGNHTHSV
                     TGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHS
                     VAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          576 a          516 c          444 g          605 t
ORIGIN      
        1 gatattactt taatcaaaag ttgacgtgcc agattcgaag taaatttata aaatatcaaa
       61 tcaatataca ttttgcacct tcgagatagt aatggctgtt actgtaacat gagttacaaa
      121 cagcttcata cccagagcta ctgatgtatc aactcgattc aacaataccc gatatttatt
      181 ccatgcctcc agcaacgatc tttcttcctc cgttgcgatt tccagatcta cagcatcctg
      241 cagtggcgca atatactcac tgaattcctg gatgtagaac tgtgtggtga cggtcttcca
      301 gccattcggc tcctgctgta tcgaagcata ccatgctatt tcaatatcgc tatgctgcgg
      361 cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata
      421 acatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta
      481 ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc
      541 ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac catctggata
      601 ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa
      661 tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta
      721 aacataaaat taaccatgat gtttttgaac cttatcccgc ggcgttacat ggtcaatttg
      781 attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct
      841 gtgtaatacg caatgcggcg caggccttaa ctgacgatgg aactctatac ggagccacaa
      901 ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc
      961 agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag
     1021 agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag
     1081 ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag acgtgctgcg gcataatccc
     1141 aatgattact ccctgacagg gttcgtaggc cactcaatat caggtgcagt tgatgtatca
     1201 acacggttca gcaacacccg atactttttc caggcttcca gcaacaaggt ttctttctcc
     1261 gttgcgatct ccagatccac agcatcctga agcggcgcta tatgctcact ggctacctgc
     1321 atcaggctgt tttttgtttc ttccgcctcc cggatccgga acagtttttc tgcttccgta
     1381 tccttcaccc aggctgtgcc gttccacttc tgaaactccc cttccgggga taaccaggtg
     1441 acattttccg gtaatgagcc gagttcagaa ataaataacg cgtcgccgga agccacgtca
     1501 taaaccgttt taccccgatg atcttcaacg agatgccacg atgactcatc actgttgaaa
     1561 acagccacga aaccagccgg aatatctggc ggtgcaatat cggtactgtt tgctggcaga
     1621 cctgtatgag gcggaatata tgcatcacct tcaccaataa attcattagt tccggacagc
     1681 agattataaa tttttatggt ccgtggttgt tcactcattc tgaatgccat tatgcaagcc
     1741 tcacaatata gttaaatgcg atgtttttga cggtgttttc cgcgttacca gcagcgttaa
     1801 cggtgatggt gtgtccatgt gagccaatcg caaccgagtg cgtatgagca ccaataccga
     1861 cagtatgtgc gtgagcacct gcagatgcag ctgtgccgct gacactatga gtgtgcgctc
     1921 ctgcagcact tgtattcaca ctggccgcag acactacctt atgtactgac ccattttgtg
     1981 accactggct gactgctgat gcgcctgtga cactatgagt atggtttccg gcacttgcgg
     2041 ctgtaccgct cacactgtgc gtatgcgccc cgatgttatt cgtggattta gtgccgtaat
     2101 caaacgacga tgtggttttc gtccccaaat ccgtactgga t
//
LOCUS       DAZKFU020000437         2112 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZKFU020000437 DAZKFU020000000
VERSION     DAZKFU020000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.439"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            68..1873
                     /locus_tag="ACHOQQ_RS24330"
                     /old_locus_tag="pgaptmp_004866"
                     /pseudo
     CDS             68..1873
                     /locus_tag="ACHOQQ_RS24330"
                     /old_locus_tag="pgaptmp_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
BASE COUNT          590 a          489 c          514 g          519 t
ORIGIN      
        1 tattcattga cttaaacaaa atgacaaaat ccatagaccc atagcctggg tactatatag
       61 gagtggtatg ggcatcaaac aacacaatgg gaataccaaa gccgatcgtc tcgctgaatt
      121 aaaaatccgt tcgccctcaa ttcaactgat aaaatttggc gctattggtt tgaatgcaat
      181 tatcttttcc cccctgctga tagctgctga tacaggaagt caatatggca ccaatattac
      241 tattaatgat ggtgacagaa ttacaggaga taccgccgat ccatcaggaa acctctatgg
      301 tgtaatgacc ccagcaggaa acacgcctgg caatatcaac ctgggtaatg atgtcaccgt
      361 caatgtcaac gacgcctctg gatatgcaaa aggaatcatt attcagggca aaaacagctc
      421 cctgacagct aaccgactca cagtagatgt tgttggtcaa acctctgcca tcggcattaa
      481 tttaattggt gactataccc atgctgactt aggcacaggc agcaccatta agagtaacga
      541 tgacggcatc attattgggc atagctcaac actaacagcc actcaattca ccattgaaaa
      601 ctcgaacggt ataggcctaa ccatcaatga ctatggcacc agtgtcgatc ttggaagcgg
      661 aagtaaaatc aagaccgatg gaagtacagg tgtttatatc ggtggtctca acggcaataa
      721 cgccaatggt gctgcgcgtt ttacggcgac agacctgaca atcgatgttc agggctacag
      781 cgccatgggg ataaacgtac agaaaaactc tgttgtcgat ctcggaacaa acagtaccat
      841 taaaaccaat ggcgataatg ctcacggcct ctggagcttt ggccaggtta gcgcgaatgc
      901 actcactgtt gatgtaactg gagccgcggc caatggcgtc gaagttcgtg gtggtacaac
      961 cactatcggt gcagatagcc atatttcttc cgcgcagggc ggtggtctcg tagccagtgg
     1021 ttcagacgcg acaatcaatt tttctggcac ggcagcgcaa cgaaacagca tcttttccgg
     1081 cggctcttat ggtgcctcgg cccagacggc aacggctgtt atcaacatgc aaaatacgga
     1141 tattacggtt gatcgtaatg gcagtctggc gctgggtttg tgggcgctca gcggcggtag
     1201 aataaccgga gacagtttgg ctatcaccgg cgcggcagga gccaggggga tttatgccat
     1261 gaccaacagc cagatcgacc tcacgagcga tctggtcatt gatatgagta cacccgacca
     1321 gatggccatc gcaacgcaac atgacgatgg ttatgccgcc agccgcatca acgcctcggg
     1381 tcgtatgctt atcaacggta gcgttctttc caaaggtggg ctaatcaatc tggatatgca
     1441 ccctgggtcg gtttggacag gttcctccct cagcgataat gtcaatggcg ggaaactgga
     1501 cgttgtaatg aataacagcg tctggaacgt aacaagtaat tctaatctcg acacgctggc
     1561 gctgagccat tcaactgtcg attttgccag ccacgggtca actgccggca catttaccac
     1621 attaaacgta gagaacctga gcggtaacag tacctttatt atgcgtgctg atgttgttgg
     1681 cgagggtaat ggcgttaata ataaagggga tttattgaat atcagcggga gtagtgctgg
     1741 taatcatgta ttggctatcc gcaaccaggg cagcgaggcc acaacgggaa atgaagttct
     1801 gacagtggta aaaaccactg acggtgcggc ctcgttcagc gcgtcttctc aggttgagtt
     1861 ggggggatat ctgtgatatg tgtgaaatca tataaaaaca catttatttt catagtgata
     1921 cagtaatgtg ttattgaatg tgttgttgga tttatggtgc aaaaaaattt tgcataagtt
     1981 cagcaacaca aagcctcccc ctttctttag cattttctac tgagcagcca tccgctttca
     2041 gacctcactt aaattacgct tattcagaga agagcgtcag aaacacgatc ggcatcactg
     2101 tttctgcaaa ct
//
LOCUS       DAZKFU020000438         2100 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZKFU020000438 DAZKFU020000000
VERSION     DAZKFU020000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.440"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..2019
                     /gene="cirA"
                     /locus_tag="ACHOQQ_RS24335"
                     /old_locus_tag="pgaptmp_004867"
     CDS             25..2019
                     /gene="cirA"
                     /locus_tag="ACHOQQ_RS24335"
                     /old_locus_tag="pgaptmp_004867"
                     /inference="COORDINATES: protein motif:HMM:NF007478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HGU4472999.1"
                     /translation="MFRFNPFIRHLLSTSIGASLFSFPVFTSAHSTLPDAAEDTLVVT
                     ASAVEQNLKDAPASISVISQDDLSRKPVHNLVDVLRDVPGVQITTDDNRKLVNIRGLG
                     GGYTLILVDGKRVSSRTAMFRNNDFDLSWIPVSTIERVEVVRGPMSSLYGADAMGGVI
                     NIITKKTGKEWSGVISGDMTLQEHRDRGDTYGGQFSAGGPLVENLLGIKVYGALSGRD
                     KDTGKQKSADGQETTRAEGFTTRNGGVDFTLTPLENHEFSTGYSFDRQDRESEGSSDK
                     NRMERQNYSVGHNGQWGLGNSELRFYSEKVNNLNTGNTHPITSWNEVIDGKYVFPFTD
                     YNHIVILGGDYRREKISDPVHLTGEKGSENSTSQYSLFAEDEWRLFAPLALTTGVRMD
                     KHENYGTNWSPRAYLVWNATDSVTVKGGWARAFKAPTLIQQSEAWLTNSCKGGCQIIG
                     SPDLKPETSESIEGGVYYSGQEGLLEGVEASVTVYRNEIDDMIANKRSKKLKEAMTLP
                     NFVGITDDGRPVFRYFNINKARIQGLESELKIPLGEQVKLSLTYTYTDGRDLSSGEDK
                     PLNDSPFHNANGRIDWTPVDDWSFYVSANYHGETRAQSSGTETQGGYTLWDAGASWKA
                     TKNITVRSGILNVMDKDLERNDYSYTVDGRRYFLSMDYRF"
BASE COUNT          638 a          380 c          538 g          544 t
ORIGIN      
        1 tacaaattat ggatataatc agaaatgttc agatttaacc cttttattcg tcatcttctt
       61 tcaaccagca taggtgcatc gttattttca tttcctgtgt tcacttctgc acattcgaca
      121 cttccggacg ctgctgaaga tactctggta gttacagcat ctgctgttga acagaatctg
      181 aaagacgcac ctgccagtat cagtgttatt agccaggatg atttatcccg taagccagtc
      241 cataatctgg tggatgtttt aagggatgtg cccggggtgc agattaccac cgatgataac
      301 agaaaactcg taaatataag gggactgggc ggaggataca ccctcattct tgtggatggt
      361 aaacgggtca gttcccgcac agctatgttc cggaataatg actttgatct gagctggatc
      421 cctgtttcca ctattgaacg tgtagaggtc gtgcgtggcc ctatgtcatc actgtatggt
      481 gcggatgcga tggggggcgt cattaatatc attactaaaa aaacaggtaa ggaatggtca
      541 ggtgtcatct cgggggatat gacgttacag gaacacagag accgtggcga tacttatggg
      601 ggacagttca gtgcaggtgg gcctttggtt gaaaaccttt taggaattaa agtttacgga
      661 gctttaagtg gccgggataa agataccgga aaacaaaaat cggcagatgg acaggaaact
      721 acacgtgctg aaggttttac aacccgtaat ggtggcgttg actttacgct tactcctctg
      781 gagaatcacg agttcagcac agggtattct tttgaccgtc aggaccggga gtctgaaggt
      841 agtagtgata aaaaccggat ggaacgacag aattattctg ttggccataa tggacaatgg
      901 ggattaggga acagtgaact ccgattttac agtgaaaaag taaataatct gaatacaggt
      961 aatacacatc cgataacttc atggaatgag gttattgatg gaaaatatgt attcccattc
     1021 actgattata atcatatcgt gatattaggt ggtgattatc gcagggagaa aataagtgat
     1081 cctgtccacc ttaccggaga gaaaggttca gaaaattcga caagtcagta ttcacttttt
     1141 gcagaagacg aatggcgttt gttcgcgccc ctggctttga caacaggggt tcgtatggat
     1201 aagcacgaaa attatggaac caactggagt ccaagagcat atcttgtatg gaatgcaacc
     1261 gattctgtta ctgtaaaagg aggatgggcc agagcattta aagctccgac cctgattcag
     1321 caaagtgaag catggctgac gaactcatgc aaagggggat gtcagatcat cggtagcccc
     1381 gacctgaaac cggaaacaag tgaaagtatt gagggtggtg tttattacag cggacaggaa
     1441 ggactcctgg aaggtgtgga agcaagtgtc actgtgtatc gtaatgaaat tgatgacatg
     1501 attgcgaaca aacgcagtaa gaaactgaaa gaggcaatga cgctgcctaa ctttgtcggg
     1561 ataacggatg atggacggcc tgttttcaga tactttaata taaataaagc taggatacag
     1621 gggcttgaat cagaactgaa aatcccgctg ggagagcagg tgaaactgtc tctcacctat
     1681 acctatacag atggacggga tttgagtagc ggagaagata agccactgaa tgacagtccg
     1741 tttcataatg caaacggaag aattgactgg actcctgtgg atgactggtc tttttacgta
     1801 tcagctaatt accatgggga aacccgggca caatcttccg gcacagaaac gcaggggggg
     1861 tatacattat gggatgccgg agcttcctgg aaggctacga aaaatataac tgtccgttca
     1921 ggtatattaa atgttatgga taaagattta gaacggaatg attacagtta taccgttgac
     1981 gggagaagat attttctgtc aatggattat cgtttttaat agtaaaccga aggctccagg
     2041 aggagccttt ataagataat attctgaaaa taaacaagac gataagaatg aagtaagatg
//
LOCUS       DAZKFU020000439         2054 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZKFU020000439 DAZKFU020000000
VERSION     DAZKFU020000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.441"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            58..1350
                     /gene="sctN"
                     /locus_tag="ACHOQQ_RS24340"
                     /old_locus_tag="pgaptmp_004868"
     CDS             58..1350
                     /gene="sctN"
                     /locus_tag="ACHOQQ_RS24340"
                     /old_locus_tag="pgaptmp_004868"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HGU4473000.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKQSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1340..1798
                     /locus_tag="ACHOQQ_RS24345"
                     /old_locus_tag="pgaptmp_004869"
     CDS             1340..1798
                     /locus_tag="ACHOQQ_RS24345"
                     /old_locus_tag="pgaptmp_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HGU4473001.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYERLKTRIKKQLLDQMLYQDELEQEEKYNGRSQEN"
     gene            1776..>2054
                     /locus_tag="ACHOQQ_RS24350"
                     /old_locus_tag="pgaptmp_004870"
     CDS             1776..>2054
                     /locus_tag="ACHOQQ_RS24350"
                     /old_locus_tag="pgaptmp_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HGU4473002.1"
                     /translation="MDEVKKIEHGKILNTDTPDNSASDLDSFLKKNKKNKEMSEIVAG
                     TILQLLRQTQDSQFSTLGKKNGQMKSIDNKSSLQVDRNPSLSLQTNGTE"
BASE COUNT          680 a          371 c          481 g          522 t
ORIGIN      
        1 ccgttaatat tctgaaaact atttaggata atgtcttgcg atatggagca tgctggtatg
       61 aaaaaattaa aattattaaa taaatacagc tacttacata gcataaacgg ctctctgatt
      121 gaagcagaac ttgacgacgt ttctgtgggt gaagtatgcg aaatttatgc gagctggcaa
      181 gcaaatgaac gtatagccag ggctcaggtc gttggctttc gcaatggaaa gacattatta
      241 aaccttatag gcagctcagt ggggcttaca cgtactgcag ttttgaaacc aacgggagag
      301 cagttaacca tacagatcag tgatgccttt cttggttcgg ttttgaatgc ctctggtcaa
      361 attatggaaa ggtttgtccc ggacactcca ggcgatagag gaaatttacg cctgattgat
      421 gaactgccac cgtcgtatca ggaacgtcga gttattaata caccactaga gacacgcata
      481 agagtcattg atggcgtact gacatgtggc attggtcaac gtgttgggat tttcgcatcg
      541 gctggctgtg gcaaaacggt tttaatgcat atgctcgtaa acaatactga agctgatgtg
      601 tttgttattg gcttgattgg cgaacgtggt agggaagtta cggaatgcgc ggaatcgctg
      661 aaacaatcag taaatgcggc aaaatgcgta ttggtttatg ccacctctga tttttcgtca
      721 gtagatcggt gtaatgccgc cctgatggcg acaaccgtgg cagagtattt tagagatcga
      781 ggaaagagag ttgtactttt tattgactca atgacgcggt atgcacgagc actacgtgat
      841 atgaaactgg cagcgggtga accacctgcc agacgtggtt atcctgcttc tgtttttgac
      901 agtttgcctc gcttactgga gaggccggga ccaacactaa aaggaagtat caccgcgttt
      961 tataccgtgt tactggaagg ggaagatgaa tcagacccac tgggtgatga aattcgctca
     1021 atccttgatg gccatatcta tctaagccgc aaactcgcgg gtcaaggtca ctatccggca
     1081 atagatgttc taaaaagtgt gagtcgagta tttggtcagg tcactgatga aaaacacaga
     1141 gataatgctg cacgggtaag gaagatcctc acaactttgg aagatctaca ggtttttatt
     1201 gacttagggg aatatcgcgc tgggcaaaat gctgaaaatg attttgctat gaatgcacgg
     1261 cctaaactca caaattggtt gaaacagtca gttaatgaaa aaatgccaat gagtgaaact
     1321 ctaaaagagc ttgaacgaat tgttaagtaa agttaatcga cttataagaa gaacagcgca
     1381 gagcctggct gcatgtgaag catcattaca aaagttaaat gcagaaaaag aaaagttagc
     1441 cgaaaaggaa cgcctgtatg atatgcaact taaaaacctg cagtcactgt tagatatgaa
     1501 agagttactc ggcgaagttg tatttcgcca ggatatattt tactcgttac gtaaagttgc
     1561 agtcattcag cagcagatcg ctgagataaa tcttgaaaag caaaaaatag cagagcggag
     1621 aaaaatactt aacaaggaaa tagttcaaca gcaagcacaa agaaaacact ggtggctaaa
     1681 aggagaaaaa tacgaacgac taaaaacgcg catcaaaaaa caacttttag atcagatgct
     1741 ttatcaagac gagcttgaac aggaggaaaa atataatgga cgaagtcaag aaaattgaac
     1801 acggtaaaat tttaaacaca gacacacccg ataattctgc ttcggattta gacagtttct
     1861 taaagaaaaa caaaaaaaat aaagaaatga gtgagattgt cgctggaacc attctccagt
     1921 tattacgtca gactcaggat agccaatttt ctacgttggg gaaaaaaaat gggcaaatga
     1981 agtctattga caacaaatca tctctccagg ttgacaggaa tccctctctt tctttgcaaa
     2041 caaatggaac agag
//
LOCUS       DAZKFU020000440         2038 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZKFU020000440 DAZKFU020000000
VERSION     DAZKFU020000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.442"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(44..367)
                     /locus_tag="ACHOQQ_RS24355"
                     /old_locus_tag="pgaptmp_004871"
     CDS             complement(44..367)
                     /locus_tag="ACHOQQ_RS24355"
                     /old_locus_tag="pgaptmp_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473003.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
     gene            complement(400..1302)
                     /locus_tag="ACHOQQ_RS24360"
                     /old_locus_tag="pgaptmp_004872"
     CDS             complement(400..1302)
                     /locus_tag="ACHOQQ_RS24360"
                     /old_locus_tag="pgaptmp_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HGU4473004.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRNNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGRDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
     gene            complement(1834..>2038)
                     /locus_tag="ACHOQQ_RS24365"
                     /old_locus_tag="pgaptmp_004873"
     CDS             complement(1834..>2038)
                     /locus_tag="ACHOQQ_RS24365"
                     /old_locus_tag="pgaptmp_004873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473005.1"
                     /translation="LTAPFITGNTQQWLCDPYPSGDAGMFLLRNGGIDFFYSFHLFAS
                     TSCDCARCIRPFEIWSLTRVSGV"
BASE COUNT          578 a          503 c          368 g          589 t
ORIGIN      
        1 tataagcctg tatattttta aatcacaaaa gatgaatgtc aagttattca acatcaatcc
       61 aaagaggaat attatgtatt cccttaacgg gttctcccag aggaaaaaca caggtccctt
      121 taatcagtat ttttcttccc gaccatctga catcacagtt aaaatctccc accacatcaa
      181 aataatccgc aatggattca tagatgtccc attcatcata gatatcccat ttatctgctg
      241 ttccttcaca ttcaaactcg gcctgatatc cggaatcaac atatcgcgcc agatagtgtg
      301 accacaaagg cgaatttttt gtaatctctt ttacagagat tattttcagc aaaatagtac
      361 aatccataat atttacccgt acgaaatcgc catgagttat tactcatagc ctaacaactg
      421 acaataaacc tgttcaatat atcgtcctct gccatcacca tgaccaagat caattgatgt
      481 cagtgcaagt gcctccttat aactgtaacc gttcataata tgatatttca tggcacttgc
      541 agcgtaactg taccgaaaac tgtgtgcagt ctcatctctg cctttcagtt ctccataacg
      601 acgtaatgta ttaagataat aatccattgc cgacttaaga ttatctctgt ctataagacg
      661 tccattatgt tcactggcat atttgagtgc ataattaaca gcatcaagaa cgttatcccg
      721 gtcaacaacc gttgtcattc tttcccgtcc ccctttcgta ccaaaaatca ctcttatttt
      781 ttcatctccc cgttgtaacg cttttttcca ggttcgtaat gatttggccg actggatagc
      841 ttcttcattt ctcagaccga ggtaacgtga cagtttaaca acagcagcca cccctttatc
      901 ttttgcttcc acacgctgaa gaatctcccg aaaacgctca tccgtgatta atttttttgt
      961 tcccttacga ctggagccgg aaatgcctaa cgctttattg ctgagacgag gatgttcggg
     1021 atcagccatc ttatatttac ctgcctgacg taacataacc cgtattgcag acatttcatt
     1081 ttcccttgtt cgcccagaaa cattattccg gcttctcata tatagctcaa tatgccggac
     1141 ttttacctgc gcggcagaac tgatctgtac attcattttt gccagacttt cagcaaagcg
     1201 gtgcatgatc ctggtgtaat cactcagctt cttgaagctg cctttgtctt ttcgaatgtg
     1261 agcaaccaga tcctgtttca gtaatttttc acgtctggac atcaacttct cctgcacata
     1321 ataattttta ccttaaacag ctgtacgttc ggcagatgag tttttctgtt gtgccgtgaa
     1381 cacaacagaa aaactcatct gcatttctcc ctgacgacac ggttaaacgt tgcgatcctt
     1441 cgggtagtga ctgcctgtgc gccagggtag cgcagattgt ttaggtattg aggggtaaca
     1501 aatcggtgtt gcacaccgac tgcagcattt gctgtacctg tctccaggtc gcccccgaca
     1561 ccacctctaa taagtggtat cgccgattcg gcatctcact acataaaact ccttaaaaaa
     1621 acaattgatt ctgcaaaaaa ccatttatgg acgttattca caaaatcgtc cgtaaatttg
     1681 cagaagaggt tgaaaaaaca ctctcacgcc gggtcacttc ttcaaatgtt aaaaaacatc
     1741 acgtgaaacg gcagttcgtg cgggttcgtt aagcagacgc gcgtgcgtca ctttgtcatc
     1801 gtgacacgct gacgaaagtg acgccgcgca gcttcagacg ccagatacac gcgtaagcga
     1861 ccaaatttca aatggtctta tacagcgcgc acagtcgcag gaagtgctgg caaacaaatg
     1921 aaaagaataa aagaagtcaa taccgccatt acgcagtaag aacattccgg catcaccgga
     1981 tggataagga tcacacaacc actgctgtgt attgcccgta ataaagggcg cggttaaa
//
LOCUS       DAZKFU020000441         2037 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZKFU020000441 DAZKFU020000000
VERSION     DAZKFU020000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.443"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            163..618
                     /gene="tssE"
                     /locus_tag="ACHOQQ_RS24370"
                     /old_locus_tag="pgaptmp_004874"
     CDS             163..618
                     /gene="tssE"
                     /locus_tag="ACHOQQ_RS24370"
                     /old_locus_tag="pgaptmp_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGU4473006.1"
                     /translation="MNRKSPSLYEILYGNFTGGLELNQVSEEEQVILSVLDNMQRILN
                     TRAGSLKHLPDYGLPDMTSILQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQ
                     PGELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            671..976
                     /locus_tag="ACHOQQ_RS24375"
                     /old_locus_tag="pgaptmp_004875"
     CDS             671..976
                     /locus_tag="ACHOQQ_RS24375"
                     /old_locus_tag="pgaptmp_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473007.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            1031..>2037
                     /locus_tag="ACHOQQ_RS24380"
                     /old_locus_tag="pgaptmp_004876"
     CDS             1031..>2037
                     /locus_tag="ACHOQQ_RS24380"
                     /old_locus_tag="pgaptmp_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HGU4473008.1"
                     /translation="MTSEVKLKTGGDPRSLPDYAALRDEISKLTHPARPDVDWRYVET
                     LCLRLYEHNGVELQTVSWYTIARMHTAGLSGLNEGLALIVALTRHHWSVMWPLNTHAR
                     LEIITGLFSRLQKTLRAMPPDERDNLPLLYQTETCLKTLSDILARHELKQSSKVALPE
                     AMVKGYITRLENQPVQGEVSSPVTLPAQALRSDAPDVQEHQPLPYSRLVYVVSETKTE
                     PTLFLQKSPLTFLKTFVTGVCTALLAVCVAIPGWQFLTQPSPAEQQLRALLAPLPDVL
                     SADQMAQLSRTPSLLNHTSEWITLSGQQITRLTELPPAYNLQRSAQLLQQLRGLFPDN
                     PR"
BASE COUNT          523 a          490 c          513 g          511 t
ORIGIN      
        1 ttaagaatac ctggagaaat taaaaaacgg agattgtttc aagagcttct tttatttaag
       61 ggtttacagg tcaaaaatgt gaatagacta gtatcatgac tgttgtatag ctgtattaaa
      121 ttagatattt aacatgacag taatacaaat tggattatta ttatgaaccg aaaatcccca
      181 tcattatatg aaattctcta tggtaatttc actggcgggc tggagttaaa tcaggtcagt
      241 gaggaagagc aggttattct ttcggtgctc gataacatgc agcgtattct gaatacccgg
      301 gctggaagcc ttaaacatct tccggattat ggtctgccgg atatgacaag cattctgcag
      361 ggaatgccgg gaacggcgca tcaactgatg cgtgtgcttt cagatgttct gctgaagtat
      421 gagccacgta ttaagcgtgt caacgtgacg atgcaggagc agactcagcc tggtgagttg
      481 cattatgtca ttgatgcaga acttaaagat gcaggactgg ttcgttacgg aacaactttt
      541 atgccggagg gcagggtgtt actgcgatat ctgaagcaac agcagtttgt ggataatgtg
      601 atgactttca gggggtgatt tatggttccc cttatccgtg tgcttaacag acataaacga
      661 gggctgaatg atgaacccgt tatttaatga catccagatg cgtctttttt atctgaacca
      721 ctctccgtac tcctggcact ggaatgtcag attcagaccc caggaagctg tctatattgg
      781 cagcgatacc tgtcatatca ccattacctg taatcagtca ggttttcatt taacccggga
      841 cggacaacgg ctgttcactg agcgttatat cagaaacctt aatgagctgt taccggttct
      901 gaaacggcgg tgggatgtga ctccggcaat tatccgcgcg gtggaatacc tttcccgggt
      961 accggtatca cactgatgtc ctttccgtaa ctgttaatca tgatttaata cagcaacacc
     1021 gaatctgccg atgacttctg aagtgaaact gaaaaccggt ggtgaccccc ggtcattacc
     1081 ggattatgct gccctgcgtg atgaaatcag caaactgacc cacccggcac gtccggatgt
     1141 ggactggcgt tacgtggaaa cgctctgcct gcgtctgtat gaacacaatg gcgtggaatt
     1201 acagacggta tcctggtaca ccattgcccg gatgcataca gcagggttaa gtggtctgaa
     1261 tgaagggctg gctctgattg tggcgctgac ccggcatcac tggtcagtga tgtggccgct
     1321 gaatacccac gcccggctgg aaattattac cggtctgttc agtcgtctgc aaaaaacgct
     1381 ccgggccatg ccaccggatg agcgtgacaa cctgcccctg ctgtatcaga cagaaacatg
     1441 cctgaaaacg ctgagtgaca tactggcccg gcatgaactt aaacagagca gtaaagtggc
     1501 tttaccggaa gccatggtga agggatatat cacccgtctg gagaatcagc ctgtgcaggg
     1561 tgaagtatcg tcgccggtaa cccttccggc tcaggcactc cggtctgatg ctccggatgt
     1621 acaggaacat cagcccctgc cgtactcccg tctggtgtat gttgtttctg aaacaaaaac
     1681 agagcccaca ctcttccttc aaaaatcccc gctcacgttt ttgaaaacgt ttgttacagg
     1741 tgtctgtacg gcattgctgg cggtctgcgt ggccattccg ggatggcagt ttctgacaca
     1801 gccatcaccg gcggagcagc aactcagggc gctgctggca ccactaccgg atgtgctttc
     1861 agcagaccag atggcgcaac tcagcagaac accctcactg ctaaatcata cgtcagagtg
     1921 gataaccctt tccggtcagc agataacgcg actgacagaa ctaccgccgg catacaacct
     1981 gcaacgcagt gcgcagctgc tgcagcaact gaggggactt ttccccgaca atccccg
//
LOCUS       DAZKFU020000442         2003 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZKFU020000442 DAZKFU020000000
VERSION     DAZKFU020000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.444"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..1861
                     /locus_tag="ACHOQQ_RS24385"
                     /old_locus_tag="pgaptmp_004877"
     CDS             80..1861
                     /locus_tag="ACHOQQ_RS24385"
                     /old_locus_tag="pgaptmp_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopB/SseC family type III secretion system
                     translocon subunit"
                     /protein_id="HGU4473009.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSSKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPAIQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     ATRLHNNLESTLLRHEMAHNKFRELSDAYSSSLDDAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVLTQKNAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CDSIKNQSEVMKEINHVRENKLNETARKYTTTTKVLKIVNECVTVATIAMSAILIVVG
                     LLAAVPSGGSSIAGALALIGGIAGAVVLGVDITCQIALGTTATGWILGKVVEGLSAAI
                     KTVDPTLLAITALLDVIGVDQDTIELVKSIYASAAASIVMATVMIGAAVICSVAIGAV
                     VSALSKTAAEEVTKEITSTIKSTIESIINSVSKNIIKVLDSVCSMIQTSVVVLKLIAK
                     ISNGLEKIGLLICETATSIMNYCVAENSADMAILQQDMSNLSKTREQMLSVLQRVDKT
                     VEQEVSQMVRVLQHRTEALKFASHSIV"
BASE COUNT          703 a          388 c          406 g          506 t
ORIGIN      
        1 gatgggaata aacatttgcc atgagttaaa taaaaaagaa ttaattacac ccggtgatag
       61 cttaaaaagg aggtgaatta tgtcggcacc gattacggga caaacgatca cgtttgaaca
      121 aatcagcgaa acattgcgta cacagtatag cgatgcggaa aaaagactgc aggacagcag
      181 caaaacgcaa gttgatccca tgcggcttaa taaaaacccg aaatcccttg ataacgatat
      241 tcgcgctcgt cttgaaaaca aaccgatgct tgctccgcct gcaatacagg tttcagattc
      301 agacaacgcc actacagcaa agacgaatga tgcccgcctg acaatgattt tgggtaattt
      361 aacaggcatt gccgatcagg atattgcaac acgattgcat aataaccttg aaagcaccct
      421 gcttcgacac gaaatggcac ataataagtt tcgtgaatta agtgacgctt actcctcatc
      481 attagatgat gcgcaaaaag ccgacgatat catgcatcag gccaataata attacaatgc
      541 ggtcgataaa aaggtgcaat cgctggagaa aaaagtcaac acattgaatc aagagttgtc
      601 acaactgcaa cctggcgatc cgcaatataa taaagtactg acgcaaaaaa atgcggctga
      661 aaaaacactc acgctgtcat tacaaaaaaa atcgttagct gagaaatcgt taaatacagc
      721 cattatggat gctgatgctg cgatcggcca aagtatggaa atttttgatg aaattcaaca
      781 gcaagaacag attaataact tcaccaccaa tatttgcctg acccaagaaa accagaaaaa
      841 tagaaacgcg acagccacat ttatcctttt gattacctca gtaatggaag ttattggtga
      901 taccaattgc gactctatta aaaatcaatc tgaggtaatg aaagagatta accatgtcag
      961 ggaaaataaa ctcaatgaaa cagcgcgtaa atatactacc acgactaaag ttttaaaaat
     1021 cgttaatgag tgtgtaactg tagctaccat tgccatgagt gcaatcttaa ttgttgttgg
     1081 tcttcttgct gcagttccta gtggtggttc aagtattgcg ggtgcactgg cgcttattgg
     1141 tggaatagcg ggcgcagtgg tattaggtgt tgatatcact tgtcaaatcg ctctaggcac
     1201 cacggctacc ggctggattt tagggaaagt tgtagaaggt ctttcggctg ctattaaaac
     1261 agttgatccc actctgctcg caatcaccgc gcttctggac gttattggtg tcgatcaaga
     1321 tacgattgaa ctggttaaaa gtatttacgc aagtgcagcc gcttctattg tcatggcgac
     1381 agtaatgatt ggtgcagctg taatatgttc tgtagcaata ggggccgttg tttctgcatt
     1441 atcaaaaaca gccgcagaag aagtcaccaa agaaataaca agcactataa aatcaaccat
     1501 tgaatctatt attaattccg tttcaaaaaa tattataaag gtattagaca gcgtatgcag
     1561 tatgatacaa acatcagtcg tagttttgaa gttgattgcc aagataagca atggcctgga
     1621 gaaaataggc ttacttatct gtgaaacagc aacatcgata atgaattact gtgttgctga
     1681 aaactctgcc gacatggcaa ttttacaaca ggacatgagt aatctatcaa aaacgcgtga
     1741 acaaatgctt tcagtattgc aaagggtgga taaaaccgtc gaacaagagg taagccaaat
     1801 ggtaagagta ttacaacacc gaactgaagc cttaaaattt gcttcccatt ctatcgtata
     1861 agagggttct aacatggcca cctatgaaat taaaaatgcg accagcacac ttaatattaa
     1921 caatctcgat aaaacaatct ttgatagaga gcatagcaaa gaaaacaaca taccaaatga
     1981 taataaaatt gtgcaatact ata
//
LOCUS       DAZKFU020000443         1977 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZKFU020000443 DAZKFU020000000
VERSION     DAZKFU020000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.445"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(33..959)
                     /locus_tag="ACHOQQ_RS24390"
                     /old_locus_tag="pgaptmp_004878"
     CDS             complement(33..959)
                     /locus_tag="ACHOQQ_RS24390"
                     /old_locus_tag="pgaptmp_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4473010.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            1116..>1977
                     /locus_tag="ACHOQQ_RS24395"
                     /old_locus_tag="pgaptmp_004879"
     CDS             1116..>1977
                     /locus_tag="ACHOQQ_RS24395"
                     /old_locus_tag="pgaptmp_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4473011.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDK"
BASE COUNT          573 a          449 c          425 g          530 t
ORIGIN      
        1 cattcttctt accgatattc tgacacatat catcaatttt cgagttttac tcttttctcc
       61 accataaaat caataaaagc acgaacttta gagggcattc tcacccgtga tgaaacataa
      121 atataaaatc caggaaatga aacagcccaa tcctcaagaa tcggctgtaa cttgcccgca
      181 ttgatatgtt cagcgattgc aaaatccaga tgttgaatga tacccgttcc attcagcgcc
      241 attcgtatca tgctttcgtc atcattagaa atatacttac ctgaaacctc tatttcgaac
      301 tcttcattac cccccacaac attagaaaac tcccatgcag agagtgcacc gctactttta
      361 tgccgcaacc gaaggcaatt atggttactc agttcctgag gcgtttcagg aataccgtgt
      421 tcttttaaat aatctggcga tgccacagta accagtttaa taagcggaga aacaggaacg
      481 gcagtcatat gctcatccag tccttgctca agacgtatgc ccacatcaca gccgtccgca
      541 ataatgttag ataaaccgtc atccataatg agttctatct ttgttttggg gtaacgagta
      601 agaaactcgc caatatgagg ctcaacaagc atccgtgctg caacacggga ggaatttatt
      661 ctcaacaggc ctgtaggttc aagttgggta tcaccaacat ctttcaaagc atcatcaatt
      721 tgccccaaag ccgacaccag cacttcatac agacgctgtc cttcctcagt aagcgacata
      781 ttgcgggtcg tacggtatat cagcttggtg ttaagacgtt cctcaagaga ttttaaattc
      841 tgggacaatg cggcccgcgt aatacccatt tcagaagcgg ctctggaaaa actgccggca
      901 cgaacaatca gcgcaaacca ggcaagagaa ggcaaaattg aaggctctat tttcatcatt
      961 gtaaacctta acttaacact gtgatagata ttagaccatt gatgataacc aatacaagtc
     1021 atactattac gccgttctcg atatgaacac gaatgccagt tgcggataat agaaatgata
     1081 tttaaatctg tttaggcaaa tccccggagc ttgaaatgaa aactgtttcg ataaaacaca
     1141 cttactggga tatagctgct gatatctatt tccctgacga ttttaatagt gaaaaaaagt
     1201 atcctgccat tatcagcgcc catccaattg gaagctgcaa agaacagaca tcgggtagtg
     1261 tgtatggtgc cgccctggca aaagctggct ttatcgtcat tgcttttgat gccagttttc
     1321 agggaagcag cggtggggaa ccgcgttatc tggaagatcc aacaatgcga gtaaaagact
     1381 tcagcattgt cgttgattac cttaccagct taccttacgt tgatgccggg cgtattggtg
     1441 tactgggtat ttgtggtgga ggtggatatg ccattaatgc cgcaatgaca gaacgtcgaa
     1501 ttaaagctat tggtactgtc acgggtgcaa actacgggcg tcttatgcgt gagggattta
     1561 cagcgtttaa tcctatcgga gcactcgaag caatggcgca acaaagaaca gacgaagcta
     1621 acggcgcaaa acttcgcgta gatgatttac tcccctcttc tccggatgca gctcttgaag
     1681 cgggattaac agaaatagac ctttttgaag ccacagaata ttatcgttcg ccacgaggct
     1741 atgcacctaa tggggtaaat cgttcgctat tctcacatca gactgttgcc gtgggttggg
     1801 atgcatttca tcttgcagaa gtcctgttaa ctcaaccaat aatggttgtg gtgggcgata
     1861 gagtgggcgc atttggtgcc taccgtgacg gctgcgaaat tattggccgc gctgcttcaa
     1921 aacataaaga gctggtcgtg gttgaaggtt attcacatta cgacctgtat gataagc
//
LOCUS       DAZKFU020000444         1970 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZKFU020000444 DAZKFU020000000
VERSION     DAZKFU020000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.446"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..548
                     /locus_tag="ACHOQQ_RS24400"
                     /old_locus_tag="pgaptmp_004880"
     CDS             <1..548
                     /locus_tag="ACHOQQ_RS24400"
                     /old_locus_tag="pgaptmp_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4473012.1"
                     /translation="INGQHVPEIILLNSHDGTSSYQMLPGYFRFICQNGCVCGQSLGE
                     VRVPHRGDVVEKVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQAALTYRYGN
                     EHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSID
                     TDIKLNRALWVMAETLLESMR"
     gene            887..1366
                     /locus_tag="ACHOQQ_RS24405"
                     /old_locus_tag="pgaptmp_004881"
     CDS             887..1366
                     /locus_tag="ACHOQQ_RS24405"
                     /old_locus_tag="pgaptmp_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4473013.1"
                     /translation="MKIVSQNTTTLSSATAPGNNQPQLVATPVPDEQRISFWQQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGKTWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECNAIMRIID"
     gene            1382..1858
                     /locus_tag="ACHOQQ_RS24410"
                     /old_locus_tag="pgaptmp_004882"
     CDS             1382..1858
                     /locus_tag="ACHOQQ_RS24410"
                     /old_locus_tag="pgaptmp_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU4473014.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT          452 a          555 c          522 g          441 t
ORIGIN      
        1 agataaacgg acaacatgtc cctgaaatta ttctgctcaa ctcccatgac ggtacctcca
       61 gctaccagat gctgccgggt tacttcaggt tcatctgcca gaacggctgc gtctgtggtc
      121 agtctctggg ggaagtgcgt gttccacacc ggggagacgt agtggagaaa gttattgaag
      181 gggcttacga ggtggtgggc gtttttgacc ggatagagga gaagcgtgat gccatgcagt
      241 cgctgattct gccgccaccg gcacgccagg cgctggcaca ggcggcactg acttaccgtt
      301 atggtaatga gcatcagccc gtcaccaccg ctgatattct gacaccgcga cgccgggagg
      361 attacggtaa ggacctgtgg agtgcttatc agaccataca ggagaatatg ctgaaaggcg
      421 ggatttccgg ccgcagtgca aaaggaaaac gtatccacac ccgtgccatt cacagcatcg
      481 acaccgatat taagctcaac cgcgcattgt gggtaatggc agaaacgctg ctggagagca
      541 tgcgctgata aaacatatcc ctgaaggcgt gattcaggta ccagaatcac ccgacgcccc
      601 ggaggcgctg ccggggtgtc gttccatttc tgatgatgac ctgtgcacct ggtgtacccg
      661 tctgctttac cgtcccggag aactcagtct gtgcagactg agtacaggca atggcatctg
      721 gccatccgtc tgtgaccgga acggttacgc gtacggctgt ccggaatttc agccgaatat
      781 cattcatccc tgatattacc gggtctcacg gtcagtgatg aacgtgatgt tgccagcccc
      841 cggcctttat ttcctctcat ttatacacat tgtaaggatt tcactcatga aaatcgtttc
      901 tcagaacaca actactcttt cttcagccac agcaccaggg aataaccagc cccaactggt
      961 tgctactccc gtccctgatg aacaacgtat cagcttctgg cagcagcatt ttggcctcat
     1021 tccacagtgg gtgaccctgg agccccgtgt cttcggctgg atggaccgtc tgtgcgaaga
     1081 ctactgcggt ggtatctgga atctgtacac cctgaacaac ggtggcgcat ttatggcacc
     1141 cgaaccggat gaagatgatg gaaaaacctg ggtactgttc aatgccatga acggtaaccg
     1201 cgctgaaatg agtccggaag ctgccggcat tgctgcctgt ctgatgacgt acagtcatca
     1261 cgcctgtcgt acggagtgtt atgccatgac ggtccactat taccggctgc gggattacgc
     1321 cctgcaacat ccggaatgca acgccattat gcgcatcatt gactgaatgg aggcagggac
     1381 aatgcagcag ctttcctttc tgcccggaga gatgacgccc ggcgagcgca gtctcattct
     1441 gcgggccctg aaaaccctgg accgccatct tcatgaaccc ggcgtggcct tcacctccac
     1501 ccgtgcggca cgggaatggc tgattctgaa catggcggga ctggagcgtg aagaattccg
     1561 ggtgctgtat ctgaataacc agaatcagct gattgccggt gaaaccctct tcaccggcac
     1621 catcaaccgc acggaagtcc atccccggga ggtgattaaa cgcgccctgt accacaatgc
     1681 cgcagccgtg gtgctggcac acaatcaccc gtccggtgaa gtcacaccca gtaaggcaga
     1741 ccgccttatc acggaacgtc tggtacaggc actgggcctg gtggatatcc gggtgccgga
     1801 ccatctgata gtcggtggca gccaggtttt ctcctttgcg gaacacggtc tgctttaacc
     1861 cgtcaccgtc acaaccacat cacacctgtt ttcactttta tcttctgtct tcagaggtat
     1921 cacactatga aaatcatcac ccgtggtgaa gccatgcgta ttcaccaaca
//
LOCUS       DAZKFU020000445         1945 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZKFU020000445 DAZKFU020000000
VERSION     DAZKFU020000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.447"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(81..842)
                     /locus_tag="ACHOQQ_RS24415"
                     /old_locus_tag="pgaptmp_004883"
     CDS             complement(81..842)
                     /locus_tag="ACHOQQ_RS24415"
                     /old_locus_tag="pgaptmp_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HGU4473015.1"
                     /translation="MKKIIAVSALAVVGMFSGLALADRGKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTSSAFTPWVSVGLGYARI
                     HHKATYTDTSWNKSGEVSNFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLD
                     AGDASVSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            1632..>1945
                     /gene="subA"
                     /locus_tag="ACHOQQ_RS24420"
                     /old_locus_tag="pgaptmp_004884"
     CDS             1632..>1945
                     /gene="subA"
                     /locus_tag="ACHOQQ_RS24420"
                     /old_locus_tag="pgaptmp_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HGU4473016.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHE
                     VYG"
BASE COUNT          542 a          462 c          411 g          530 t
ORIGIN      
        1 accctgcagt ataattacgg cagggagttt ccgtttaatt acagaagaca ggtaattatt
       61 atctttcagg agtagtttcg tcagaagtta taagtcacac caagcatgat gtcatgactt
      121 ttaacatcaa cttctgattt atatttatcg ccccactcat ccttgtaact cacactggca
      181 tcacctgcat caagatagcg atagctgagg tccagagcga tatccggggt tacgtcatag
      241 cggacacctg ccccaaaact ccatgcaaaa ttgttatcat atcccgggta atgcaacgca
      301 gaaaaattgc tcacctcacc agatttattc caggaggtat cagtatatgt tgccttatga
      361 tgtatccgtg cataacccag tccaacagac acccatggag tgaatgcact gctggtgaat
      421 gcactgctgt ttctgaagtc ataatacgta ttcaccatca gagtattcac actgagcctg
      481 ttctgtgtgt cttcccgacc accaccgacc atcggagagt accatgtatc cagtgtataa
      541 cgggagtccg cagctccgcg gccatagaat tccacttccg tgcgcacagg aacattgtaa
      601 tgctgataaa aatcatagcc caccgcaagg ccggcaccga ataccgtatc gttcttatcg
      661 ccgcccttat acttataaac atcgtcccca aagtcctgac gaaagcgctg gtcagtctga
      721 gtcacaaccg aagcaccagc tttaccggtc atataaaagc ctgttttgcc tctatcagcc
      781 agggccaggc ctgaaaacat gcccaccaca gcaagcgctg aaaccgcaat aatctttttc
      841 atttcagtta tctccgctat cactttcccc gaacgataaa cgtggttgct tattctaaag
      901 ctatgcaaaa gacagggtaa aaagagaaaa atcgatcgaa tcgatcgaca aaatcgatca
      961 tgcatgatga agatttctta tcgaatacat aaaaatagtg atggcaatcg tggcaggagc
     1021 ttacgtcagg ccctgaagta ctgacacagg cattgatggc aggtgatata aggagtgctc
     1081 cagggaatga ctcccgactg acccaggcac tgtcccgttg tcaacccgta tggttactgc
     1141 tctgactcat ttcctgtcgt cagacgttca actctggacc actggttatg acgacaggtg
     1201 tcgctcattt gtcagcacta taacggtcaa agatcgtggc attgacacat tctccagagg
     1261 taaatttctc aagaggtaac aactctgcca ggaggatatc tgcagagagt tacattcgtt
     1321 tgggttcata tctgtgccgc gcgttttcct tccatcagtg aacaaacatt cagtcccatg
     1381 acgaccgggc aaatagcgtg ttcacagata ttgctgtcca ggtacgaagt accataatgg
     1441 atattggtaa gttattgggt gaaccccctc catgaaaaaa ccaaatataa aaatgaatgt
     1501 ttcgggagag acttcactcg ctttattgat agtgattgtt tatcttttac tgaagacagg
     1561 atatttcaga tgtgatatcc ctgtaacata ttgaccagca atacttttta tttgtaccac
     1621 tggaggatat tgtgctgaaa aatttacggc tgcatattct gttcctgctt tttatctcag
     1681 tgtcagtcag ggcggaaaaa ccatggtatt ttgatgcaat tggtctgaca gaaacaacca
     1741 tgtcgctcac agacaaaaat accccggttg tcgtatctgt tgttgattcc ggagtggcat
     1801 ttgtaggagg cctcagcgac agcgaatttg ccaaattcag ttttactcag gatggttcgc
     1861 cattcccggt aaaagagcct gaagctttat atattcatgg tactgccatg gcttcactca
     1921 ttgcctcacg tcatgaggtt tatgg
//
LOCUS       DAZKFU020000446         1944 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZKFU020000446 DAZKFU020000000
VERSION     DAZKFU020000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.448"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1149
                     /locus_tag="ACHOQQ_RS24425"
                     /old_locus_tag="pgaptmp_004885"
     CDS             <1..1149
                     /locus_tag="ACHOQQ_RS24425"
                     /old_locus_tag="pgaptmp_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4473017.1"
                     /translation="TPANHAARFTEVVDQYRADLADMAGQCVGGSADSVPWICGDTTY
                     FWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPDIGYYGS
                     KWRTDRSTWTSQDRASHFSSWARRGIISDRLATAILSCAGKSSAFVNGTAGVVVPDRP
                     VTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLVDDSGNKALRIEKNNSAKSWSMYC
                     DIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKYAFGLYWRIAEWPGEGGEGYLSS
                     FFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWHTLAFKFKGSNSINVTPVLDGVDG
                     QAFDLVKWANTANGLNRFVITDITGSAETYPVLIDTVEVKANKAGAAA"
     gene            1289..1471
                     /locus_tag="ACHOQQ_RS24430"
                     /old_locus_tag="pgaptmp_004886"
     CDS             1289..1471
                     /locus_tag="ACHOQQ_RS24430"
                     /old_locus_tag="pgaptmp_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4473018.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            1497..1769
                     /locus_tag="ACHOQQ_RS24435"
                     /old_locus_tag="pgaptmp_004887"
     CDS             1497..1769
                     /locus_tag="ACHOQQ_RS24435"
                     /old_locus_tag="pgaptmp_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4473019.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            1846..>1944
                     /locus_tag="ACHOQQ_RS24440"
                     /old_locus_tag="pgaptmp_004888"
     CDS             1846..>1944
                     /locus_tag="ACHOQQ_RS24440"
                     /old_locus_tag="pgaptmp_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HGU4473020.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSP"
BASE COUNT          538 a          415 c          538 g          453 t
ORIGIN      
        1 acgccagcga atcatgcagc ccgttttaca gaagtggtgg atcaataccg tgcggacctt
       61 gcagatatgg cggggcagtg tgtgggtggc tcagctgaca gtgttccctg gatttgtgga
      121 gacacaacat atttctggaa gcagaagaac gaaacggcgt accagacggt gtacggtagt
      181 tacaaaaata aaacggaaaa gaatatccat ttcgtgccgt tcatgaccga tgagaacggg
      241 gtgaatgtgc cgacgaacaa accggaagaa gacccggata ttccggatat cgggtattac
      301 ggttcaaaat ggcgaacgga cagaagcacc tggacatctc aggacagggc cagtcatttc
      361 agttcatggg ctcgccgtgg gattatttcc gaccgtctgg caacggcgat tctgagctgc
      421 gcgggtaagt cttctgcgtt tgttaatggt actgccgggg tggttgttcc agacagaccg
      481 gttaccacct cagagtctgt aattttttac gatgccaaaa aagcttcaga caatcagctg
      541 aaaccttatg gctgggacgg tatggatggc agacgcacac tggttgatga cagcggcaat
      601 aaagctctgc gaattgagaa aaataacagc gcgaaatcct ggtcaatgta ctgtgatatt
      661 gctgcagaca aggcaaaact tttactggaa aaaggcgggg aaattgctgt ccggtttaaa
      721 atccccgaaa acgtcaatct tgagacaacc agaaacaagt atgcctttgg tttgtactgg
      781 cgaatagcgg aatggccggg tgagggtggt gaaggctatc tgagttcttt ctttgtccag
      841 acagataaag ccagtattga tgttgcatac catcatacag ttaatcaaca aaaagaactt
      901 ggcacgtttg gcgcattcga ccatgactgg catacgcttg catttaaatt taagggcagt
      961 aacagcatta atgttactcc ggtgcttgat ggtgtggatg gacaggcgtt tgacctggtg
     1021 aaatgggcca atactgctaa tggactcaac aggtttgtca ttacggatat tacaggtagt
     1081 gcagaaacct accctgtact tattgatacg gtggaagtta aagcaaacaa agctggagca
     1141 gccgcataat tgctaaaaaa agccgccagc ggcaggaatg gaagctggcg gaggtaatcc
     1201 caatggagaa tgtaaagaaa agatgctttc gtacattggt tttttaaatg aaaacagttc
     1261 tcattgtcaa ccataacggt aagaaattat gatatttatt catcaggtga tgctgtactt
     1321 ctgtacggcg gtctgtgtgc tgtatcttct ttcgggtggg tacagggcag tgcgcgattt
     1381 ctggcgcagg cagattgata aaagggccgc tgagaaaatc agcgccagtc agtcagccgg
     1441 aagcaaaccc gaagagcccg ttactcctta acaacccctt tcagcgagaa aatcccatgt
     1501 cagaaattac atccctggtc actgcagagg cagtgaagga agtcctgcgc tctgaagaag
     1561 tccggagcgc actgaaacag aaacttcgcc agaatcttga ggcgcgtctt gatgcagaag
     1621 ttgatgccat tctggatgag ctgcttggtg tacaggcaga gccaccgact gaagcgggag
     1681 ataccaccgc agagagcggt gaagttcagc ctgaatcacc ggtcgccgat gcgactgaac
     1741 ctcaacccga atcggtcatg atgctgtaac gaggagtcag ggccatcagt aaacggctgc
     1801 tggccttttt catgttgtga gcttccggat tgcgggagac ggggtatgta ccagatggaa
     1861 aaaatcacaa caggtgtgtc atacaccacg tcagcggtgg gaacgggcta ctggttcctg
     1921 cagttgctgg acagggtttc cccg
//
LOCUS       DAZKFU020000447         1938 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZKFU020000447 DAZKFU020000000
VERSION     DAZKFU020000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.449"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1938
                     /locus_tag="ACHOQQ_RS24445"
                     /old_locus_tag="pgaptmp_004889"
     CDS             <1..>1938
                     /locus_tag="ACHOQQ_RS24445"
                     /old_locus_tag="pgaptmp_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HGU4473021.1"
                     /translation="LISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDSLSEKWSLAG
                     VTLHNYGVNGYRNNWLLLPEDYIRNIIVADFDPIISFNKNSKEHMSWTYDAAKGVGRI
                     QQDDQQFVMHGNLNGNLNAGKNLYFTGANGIIDLKDNVNQGAGYLQFADDYTVTTSNA
                     SSWFGGGIIVNSGTTVKWGINGVSGDDLHKVGDGTLIINGTGKNEGGLKIGAGTVILE
                     QKAKNNDSTAFSSINISGGNSRVKLSGDNQIIPDNVSWGFRGGYLDINGKNTEFSRLQ
                     AVDYGAAIINSSTEKSLLTLNLSPLKKDEIAVSVKALDMNAIFHGGYGTTGDLYKTTF
                     YGPTQYYLLKKPKFGSVLMGSLKNTSEWQFAGTDLNQAVDMAKNNKLTSSAQASYLYH
                     GKLLGNMDIVIPELTGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQS
                     DWENREFSLNNLNLNNADFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDV
                     ISVEGKSAAAGTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASS
                     FINSSLNIHKGGALTAQGGLFTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGF
                     KLMSDSSVLKARDQASVVGDII"
BASE COUNT          579 a          363 c          465 g          531 t
ORIGIN      
        1 ttaatatcaa gctctccagg tgacacctat aataaatcac agggaccatt agccagttat
       61 gggcagatgg gtgacagcgg gtcgccatta tttgcttatg attctctcag tgaaaaatgg
      121 tctctggctg gagtcaccct gcataactat ggggtgaacg gttacagaaa taactggttg
      181 ttattacctg aggattatat cagaaatatt attgtagccg atttcgatcc cattatttca
      241 tttaataaaa acagtaaaga acatatgtca tggacatatg atgcagccaa aggcgtgggc
      301 cgtattcagc aggatgacca acagtttgtg atgcatggta acctgaatgg taatctgaat
      361 gcaggtaaaa atctttattt tacaggcgca aatggcatta ttgatttaaa agataatgtt
      421 aatcagggag ccggatatct tcagtttgcg gatgattata cagtaacaac gagcaatgcc
      481 agttcatggt tcggaggagg cattattgtc aattcgggta caactgtaaa atggggtata
      541 aacggggttt ccggagatga ccttcataaa gttggggacg gaacgcttat tattaatggc
      601 acagggaaaa atgaaggggg actgaagatt ggggctggta ctgtaatcct tgaacaaaaa
      661 gcgaaaaaca acgattcaac ggctttttct tccattaaca tcagtggggg aaactcgcgc
      721 gttaaactct caggagataa tcagattata cctgataatg tttcctgggg attccggggt
      781 ggatatttag atataaacgg aaaaaacaca gagttctccc gcttacaggc tgttgattat
      841 ggggctgcaa ttattaacag cagcacagag aagtcattat tgacgttaaa tctgtcacca
      901 ttgaaaaaag atgaaatagc tgtgagcgtg aaggcgttag atatgaacgc catctttcat
      961 ggaggatacg ggacaaccgg cgatttgtat aaaacaactt tttacggtcc gacacagtat
     1021 tatctgctga aaaagcccaa atttggcagt gtgctgatgg ggtcactgaa aaacaccagc
     1081 gaatggcaat ttgcaggaac agacctgaat caggccgttg atatggcaaa gaataataaa
     1141 ctgacatcaa gtgcacaggc ttcatatctt tatcacggta agctcctggg gaatatggat
     1201 attgtcattc cggagcttac aggcaatgat attttaacgc ttgatggctc tgtcagtata
     1261 tcaggagata tgtcaaaaca ggacggtgct cttatcttcc agggacaccc ggttattcat
     1321 gcagggcaaa ctgtttctgc atcgcagagt gactgggaga acagggagtt ctcactcaac
     1381 aatctgaatc ttaataatgc ggacttcagt ctgtcccgta atgcatttat gaacgggaat
     1441 atcagggccg ttaaccagag cactgttatt atcggcggag atacagtctt tactgataaa
     1501 aatgacggaa caggtaatga tgtcatcagt gttgaaggga aatctgctgc cgcaggaaca
     1561 tcctcctata cagggcatat cactctggag caaaaatcag cactggatat ccgcgataat
     1621 tttcgtggcg gggttacgtc tgaagacagt catatcaatg tttcttcatc ttcagtcctg
     1681 ttctcagatg catcgtcatt tataaacagc tccctgaata ttcataaagg aggtgcgctg
     1741 accgctcagg gagggctgtt tacaagtgga agcattgata ttggtgacgc ttcccttctg
     1801 cttaccggta caccagtgaa ttcagatgat gctgcttttt taccgaccat caatatggct
     1861 gatggcggat ttaaactgat gtctgattca tcagtactga aagccagaga ccaggcatct
     1921 gttgttggtg atattatt
//
LOCUS       DAZKFU020000448         1904 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZKFU020000448 DAZKFU020000000
VERSION     DAZKFU020000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.450"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            133..1872
                     /locus_tag="ACHOQQ_RS24450"
                     /old_locus_tag="pgaptmp_004890"
     CDS             133..1872
                     /locus_tag="ACHOQQ_RS24450"
                     /old_locus_tag="pgaptmp_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4473022.1"
                     /translation="MKSVKLSLLAVVVATIVSPSAFADDTVEAAATELTAIHPGMSHD
                     EIDQKINRFMIRTGNTEAAQDYLFSHGYMDPQTGDNIYPASTAVQTDSNITDQKQADR
                     DAAQDTAIQNAHNIASIAAANADDAKKALTTAQSAIDANKGAIADTQKDVADVKASAA
                     HANTNANTALMNGLKLSGAVSDNKNQIEKNSSSIAEQQEQMALSEQNQIHRDAAQDSS
                     IQDAQHSANWASLKADDAHHAIMVAQTDIDANKSAISDTQKDVAAVRTDVSAMKDDVA
                     DVKASADHANANANTALMNGQKLSGVVSDNKNQIEKNSTAITEQQEQITATQKTVAAT
                     GDVKTAARYQSMINALQTATADAQQSQSEAQQTQITEQQKQIAATEKTVAAIGDPQST
                     AHYQKMINAKLTAQNEANERSTADQEQRINELASNVATQQQIDSVQYGEQIRNLAQDS
                     AQAHEQIESLTQDSAQTHQQLTNTQKRVADNSQQINSLNNNFSSLKHEVEDNRKEANA
                     GIASAVAIASQPQVKTGDFMMVSAGAGTFNNESAVSVGASFNAGIHTVIKAGVSADTQ
                     SDFGAGVGVGYSF"
BASE COUNT          617 a          459 c          428 g          400 t
ORIGIN      
        1 aaaaacgcgc tttacatcac acaaatggcg gcgtagattt cgattaaatt gcaacgcaaa
       61 ttaactccaa tagaaatatt attagtttct attagaaacc aaaataaact taatttgatc
      121 gagaaaaaat aaatgaaatc tgtaaagttg tctttactgg cagtcgttgt tgctaccatt
      181 gtgagcccat ctgcttttgc agatgatacg gttgaagcgg cagcgacaga actgacagcg
      241 attcacccag gtatgtctca tgatgaaatc gatcaaaaaa tcaatcgctt catgattcgg
      301 acaggtaata cagaagccgc gcaagattac ctgttttctc atggatatat ggacccacag
      361 acaggcgata atatttaccc tgccagtaca gccgtacaga ctgacagcaa catcaccgac
      421 caaaaacagg ctgatcgcga cgcggcgcaa gataccgcaa ttcagaacgc acacaatatc
      481 gcaagcattg cggcggcaaa tgcagatgac gccaaaaaag cgcttaccac tgcgcaatct
      541 gcaattgatg cgaacaaggg agctattgct gatactcaga aagatgtcgc agatgttaaa
      601 gcaagcgccg ctcatgccaa cacaaacgcc aataccgccc tgatgaatgg cctgaaactc
      661 tccggtgccg tgagcgacaa caaaaaccag attgaaaaaa acagctcctc tattgctgaa
      721 cagcaggaac agatggcgtt aagtgagcag aaccaaatac atcgcgatgc cgcacaggac
      781 tcgtcaattc aggatgccca gcactctgct aactgggctt cgcttaaagc cgatgacgcg
      841 caccatgcca ttatggtggc gcaaacggat atcgacgcca ataagtcggc catttccgac
      901 acacaaaaag atgttgccgc tgtccgcacc gatgtgagtg caatgaaaga tgatgtcgca
      961 gatgttaaag caagtgccga tcatgccaac gcgaatgcta ataccgcgct gatgaatggc
     1021 cagaaacttt ccggtgtcgt cagcgacaac aaaaaccaga ttgaaaaaaa cagtacggcg
     1081 attaccgaac agcaggaaca aatcactgcc acgcaaaaaa ccgttgccgc cacgggtgat
     1141 gtaaagaccg ctgctcgtta tcagagcatg attaacgctc ttcagacagc cactgctgat
     1201 gcgcaacaat cacagtctga agcccaacaa acgcagatta cagagcaaca gaagcagatc
     1261 gcagccacag aaaaaacggt cgctgcaatc ggcgatcccc agtcaactgc gcattatcaa
     1321 aagatgatta atgccaaact gacggcgcaa aatgaagcca atgaacgttc aaccgctgat
     1381 caggagcaga gaattaatga actggcttcc aatgtcgcga cgcaacagca aatcgatagc
     1441 gtgcaatacg gtgaacaaat tcgaaacctt gcgcaagact cggcccaggc acatgagcaa
     1501 atcgaaagtc tgacacaaga ttctgcccaa acgcaccagc agttaacaaa cacgcaaaaa
     1561 cgtgttgcag ataacagcca gcaaatcaat tcgctcaata acaacttcag ttcgttgaaa
     1621 cacgaagtgg aagataaccg taaagaggct aatgcgggta ttgcgtcagc cgtcgctatt
     1681 gcttcacagc ctcaagttaa gacaggtgat tttatgatgg tgtctgccgg ggcgggtaca
     1741 ttcaacaatg aatctgcggt atctgtcggt gcttcgttta atgctggaat tcatacggta
     1801 attaaagcgg gagtctctgc ggatacacaa tctgatttcg gcgcgggtgt cggcgtgggg
     1861 tattcgttct aattttaata gcaatttctt attatactct aaat
//
LOCUS       DAZKFU020000449         1900 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZKFU020000449 DAZKFU020000000
VERSION     DAZKFU020000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.451"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(84..302)
                     /locus_tag="ACHOQQ_RS24455"
                     /old_locus_tag="pgaptmp_004891"
     CDS             complement(84..302)
                     /locus_tag="ACHOQQ_RS24455"
                     /old_locus_tag="pgaptmp_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013575645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473023.1"
                     /translation="MLIYNVFGRHIGVKREGDHWLVFRADLTERKFSRLYDIVIPDDM
                     TEDEIPGWLSDIFHEAATERHPDVKRIR"
     gene            complement(533..630)
                     /locus_tag="ACHOQQ_RS24460"
                     /old_locus_tag="pgaptmp_004892"
                     /pseudo
     CDS             complement(533..630)
                     /locus_tag="ACHOQQ_RS24460"
                     /old_locus_tag="pgaptmp_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1161..>1900
                     /locus_tag="ACHOQQ_RS24465"
                     /old_locus_tag="pgaptmp_004893"
     CDS             1161..>1900
                     /locus_tag="ACHOQQ_RS24465"
                     /old_locus_tag="pgaptmp_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4473024.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWM"
BASE COUNT          456 a          443 c          510 g          491 t
ORIGIN      
        1 gcaggatatc ccctgtaacg aagtttgcgt aacagcattt tactctacga gtttgccagc
       61 ctcccccagt gggctggctt tttttatctg atgcgcttca catcagggtg acgttctgtt
      121 gccgcttcat ggaaaatgtc gctgagccag cccggtatct cgtcttctgt catatcgtct
      181 ggaatgacaa tatcgtataa ccgggagaat tttcgttctg tcaggtctgc ccggaagacc
      241 agccagtgat caccttcacg tttgacacca atatgtcgac caaagacgtt gtagattagc
      301 actatcgacc tctttcagga attgtgtaac tgagatgaca gattcagatt tcgtcaataa
      361 atgcaaaatg tcaatcctga gatcatggtg agctatctgg cggtttttta tatctgttta
      421 acgggggatg ttacatacct cacaattcag tcagttgaag gttgtctgcc cggatgagaa
      481 tttgttagaa aatttctgca tggtgaatcc ccctgagcgg aggggcatat cagcgacagg
      541 tggttggtta tatcccttat ctggtgcggg ttcaggtgct gatactgaac tcaccgggag
      601 gcacccggca ccatgcaatg aacggtacat agcgctactc ttcagcccct ctccggaggg
      661 gctttttaat gaggggaaga cactgggcag tgaaatgtta aatcactcac aattcaggta
      721 gttgacggtt gtctgcttgg ctgagaattt gtaaaaaaaa tcctgcatgg tgaatccccc
      781 tgtgcggagg ggcgactggt gaatggtatg atctctttga tgatcgtaag cgagaatacg
      841 cgggtttggt ggcaccaggc cgaactcacc gggaggcacc cggcaccatg cagtatacag
      901 agattaggca tatatcaggg cccctcatag caggggcctt tttacatgca aaaaaaagcc
      961 gccccgggaa gagcggttgg caagaagaaa agcaatatga acaattaatt aacgctgcga
     1021 ataatacctt acagtaatca ccctgcgcaa ctgtgaggag tgtatttctt ttttgcgggt
     1081 ggttgtcttt ttcccttgtg tttcgggact tccgctcact ccttatctta ttcagtacat
     1141 tatcccggcc gggaggattc atggcattta aacactatga cgtggtcagg gcggcgtcgc
     1201 cgtcagacct tgcggaacga ataactcaaa aactgaagga agggtggcag ccttatggta
     1261 gtgcgctgat ttcgacagct ggttatggtg cggagttcat ccagccagtt gtgagtgagg
     1321 ggagcatctc atcaccagag gagccaggca accgtccgac gacctcagcg ccttctgttg
     1381 cgccagaata ttactatgtg atcgcgcttg ctggtcagtc caatggtatg tcatacggtg
     1441 agggactgcc attaccggat acattcgaca gccctgatcc acgtattaaa cagttagcgc
     1501 gtcgcagtac ggtgacaccg ggaggtgctg catgtatcta taacgatatt atcccggcag
     1561 atcactgtct ccatgatgtg gaggatatga cagggcttaa tcatcccaaa gcggatttat
     1621 cgaaaggtca gtacggctgt gtgggacagg ggctgcatat cgccaaaaag cttctgccat
     1681 ttataccggc gaatgcgggc attcttcttg ttccgtgctg ccgtggtggt tcagctttca
     1741 ccaccggaac cgatggcaca tacagtgaca ctactggcgc ctcagagagt tccacccgct
     1801 ggggggtggg caggccgctg tataaggacc tcattggtcg tacaaaagcc gcgctggcga
     1861 agaatccgaa aaatgtgctg cttgccgtgg tatggatgca
//
LOCUS       DAZKFU020000450         1897 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZKFU020000450 DAZKFU020000000
VERSION     DAZKFU020000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.452"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..834
                     /locus_tag="ACHOQQ_RS24470"
                     /old_locus_tag="pgaptmp_004894"
     CDS             1..834
                     /locus_tag="ACHOQQ_RS24470"
                     /old_locus_tag="pgaptmp_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU4473025.1"
                     /translation="MMKLGFNEATCLRNSSLEKDLVLAERFGYDYIEIRLDMLQTWLQ
                     EHTLVELADIFSVSHLKPWGYNSLEDITFCDAGRWSEIRQQLVLACYAGEVVGGDCLV
                     VVPTIREGAHFSDRDTVRDSVKRLREMSALAAESQMRLAFEPIGSAGCCVRSLAMAME
                     IVDAVDRSNVGLAIDAFNLYLYDQWQDLSTLRQVPVEKIFVYHIDDADRLPVAVLDHC
                     HRLFPGNGAIPLYMITHELVKKGYTGICSLELFNPGYWQMAASEVFAIGAEKTRPFLA
                     D"
     gene            complement(1050..1220)
                     /locus_tag="ACHOQQ_RS24475"
                     /old_locus_tag="pgaptmp_004895"
                     /pseudo
     CDS             complement(1050..1220)
                     /locus_tag="ACHOQQ_RS24475"
                     /old_locus_tag="pgaptmp_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          480 a          395 c          488 g          534 t
ORIGIN      
        1 atgatgaaac tgggattcaa tgaagcgacc tgtctgcgta attccagcct ggagaaggat
       61 ctggtcctag cggaacgttt tggatatgac tatattgaga tccgtctgga tatgttgcag
      121 acgtggctgc aggaacacac tctggttgaa ctggccgata ttttttctgt aagccatctt
      181 aagccatggg gctacaactc gctggaagac atcacatttt gtgatgccgg gcgctggtcg
      241 gaaatacggc aacaactggt tttagcctgt tatgcagggg aagtcgtagg tggtgattgc
      301 ctggttgtag tgccgaccat tcgtgaaggt gctcattttt cagatcgtga cacggtaaga
      361 gattcagtga aacgtttgcg tgagatgtca gcattagcgg cagagagcca gatgcgactg
      421 gcctttgagc ctatcggctc tgcgggatgt tgcgtacgca gtctggcgat ggcgatggag
      481 atcgtggacg cagttgatcg gagtaacgtt ggtctggcaa tagatgcctt taacctttat
      541 ctgtacgatc aatggcagga tctgagtacg ctgcgacagg ttccggtgga aaagatcttt
      601 gtctatcata ttgatgatgc tgaccgttta cctgtagctg tgttggatca ttgtcatcgt
      661 ctgtttccag gcaacggtgc gataccactg tacatgatca cccatgaact ggtgaaaaaa
      721 ggttatacag ggatctgttc actggagttg tttaacccag gttactggca aatggcagcc
      781 agcgaggtct ttgctatcgg agcggaaaaa acgcgacctt tcttagcgga ttaacgctga
      841 cgtaactgca aatgaactgt cgctgtgccc ggtaataagc aggtgatcag aataaatctc
      901 ctccggcata gccggaggtt tttcagatgt gtcttaaaga ttcgttttct gcctcgccct
      961 ggctgacagg cgcatatgat ctgacatttt cattcaactt ccttacttac gccccgtaaa
     1021 ttcacaagca tatggatatg atccgctcag cattcagctt ccagaatccg tacacttttc
     1081 cactcacaca gttttctcaa aatattgcct ggtgctcttg cacttcgctt ttctctgtag
     1141 aacatctgtc ttcggtattt tggcgcaaaa actatgtgat atttacagtt ccatcgggtg
     1201 ttcgttaagc cctttttcat tcacccattg gaatcccctt ttgatttctt gtttgacgct
     1261 tacagttggt agactgcaag gtgttttaac aaatcaaaaa ggggttttga caacagcctc
     1321 aaagttggaa gttttactga accactaggc tggctggtgg ttttctataa ataacaagaa
     1381 agatctgttg gtagtcaatg aatggcgtca gcaggcccta tgtcaatccg gtcagtcctc
     1441 aacttagagc ctgaaatgct gaataggggc caggggcggt tccgcggatt ttgggagagg
     1501 ccaagtgtac acctcatccc agtaacggaa aatttaactg attaataatt aattaacgaa
     1561 ttcagttctg tatatatcag gcctgatact cattttggtt acttaaatgg gtgaatgcag
     1621 aaaggttgct ctggtggtca acgtgatgac aaagacaggt aagacaggga ctcactaaac
     1681 ctaaatgggg ttttgggctt gaatcccgcg cagtaaatga ggtgtgagtc agcgaattac
     1741 ataggggctc gcagctttat ggttgcaata aagccggatc cccgggggaa tagatttaag
     1801 cccagcaggc acgtcgacca acgaatatcg tcatcataga gctggcaaat aagattgcca
     1861 ggatcgcatg ggcagtgttt atgcgtggtg aaaaact
//
LOCUS       DAZKFU020000451         1892 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZKFU020000451 DAZKFU020000000
VERSION     DAZKFU020000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.453"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..279
                     /locus_tag="ACHOQQ_RS24480"
                     /old_locus_tag="pgaptmp_004896"
     CDS             52..279
                     /locus_tag="ACHOQQ_RS24480"
                     /old_locus_tag="pgaptmp_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473026.1"
                     /translation="MACNVFAFSNSGGGSETSYKEPEKTSAMTTTHSTKFQPSQAILF
                     KMREDAPPLNLTEETTPPFPTKASYLIHPVR"
     gene            complement(356..598)
                     /gene="yfdY"
                     /locus_tag="ACHOQQ_RS24485"
                     /old_locus_tag="pgaptmp_004897"
     CDS             complement(356..598)
                     /gene="yfdY"
                     /locus_tag="ACHOQQ_RS24485"
                     /old_locus_tag="pgaptmp_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGU4473027.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            951..1871
                     /gene="lpxP"
                     /locus_tag="ACHOQQ_RS24490"
                     /old_locus_tag="pgaptmp_004898"
     CDS             951..1871
                     /gene="lpxP"
                     /locus_tag="ACHOQQ_RS24490"
                     /old_locus_tag="pgaptmp_004898"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGU4473028.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
BASE COUNT          525 a          468 c          424 g          475 t
ORIGIN      
        1 tctatgaaag taaacttaat actttttagc ttatttttat tggtctctat tatggcatgc
       61 aatgtttttg cattttctaa ttctggtggt ggtagtgaga cgagctataa agagccagaa
      121 aaaacgtcag cgatgacgac aactcactct acaaaatttc agccatcaca ggcgattttg
      181 tttaagatga gagaagatgc accaccatta aacctcacag aagaaaccac gccccctttt
      241 ccgacaaagg cgagttatct tattcatcct gtgcgatagc gtctgtcaca tggcaggtga
      301 gcaatcatgt ctgatgctga tgtgaacacc tgcccaaatg agccatcctg acccactacc
      361 gcctgccata ccccaccata aagcgaatca tcaagccaca agccggggca agaaacataa
      421 atatccccgt caccagttca ttaccgccag ttaaccacat ggtgaagtaa taaatgagtg
      481 cagcgaggat caccatgccg aagaaagaag agaccgccga taccacgttc agtacctgac
      541 cgatgtagcc gcttacgcag acaatacaca gggcgagaaa catccataaa taaatcatag
      601 ctcaacctcc tgccgcgcta aaaaacctgt aacttcagtc actttaatag taagacaatc
      661 cttaacctct ggcaattttt gctaagcatc aatgaaaaca gcatgttaaa tgcaacacta
      721 ttgcatgcgg aaaatttttt actttgcacg attaataatc agtagctgaa agcagtcagc
      781 cagtgataaa gtagagccac cgcattcatg ctcctcgtgg ttatgtcctg actagtcttt
      841 acactcttta caggaaccat tgtcgtacat gatggcccaa ccaattgagt gtaccgctgt
      901 cgacatggaa tcttctgcga tacaacaatt cgtatctaca gaaggtaact atgtttccac
      961 aatgcaaatt ttcccgcgag tttctacatc ctcgctactg gctcacatgg tttggacttg
     1021 gtgtactctg gctttgggta cagcttcctt atcctgttct ctgctttctc ggcacgcgta
     1081 ttggcgcaat ggcgcgacca ttcctgaaac ggcgtgaatc tatcgcccgt aaaaacctgg
     1141 aactttgctt cccgcagcat tctgcggaag aacgcgagaa gatgattgcc gaaaactttc
     1201 gttcactcgg catggcgctg gtagaaaccg gcatggcgtg gttctggccc gacagtcgcg
     1261 tacgtaaatg gtttgatgtt gaagggctgg ataaccttaa acgcgcacaa atgcaaaatc
     1321 gcggcgtaat ggttgtcggc gtccatttta tgtcgctgga actgggcggc cgcgtgatgg
     1381 gactttgtca gccaatgatg gctacctatc gtccacataa taatcagctg atggaatggg
     1441 tgcagacccg tggtcgcatg cgctctaaca aagcgatgat cggcagaaat aatctgcgcg
     1501 gcattgtcag tgcactgaag aaaggtgaag cggtatggtt tgctcccgat caggattatg
     1561 gtcgtaaagg cagctccttc gcgccgttct ttgcggtaga aaatgtcgcc acaaccaatg
     1621 gcacctatgt tctctcccgt ctctctggcg cagccatgtt gaccgtaacg atggtaagaa
     1681 aagcggatta cagcggatat cgtttgttca tcaccccaga gatggaaggc tacccgacag
     1741 atgaaaatca agccgctgcc tatatgaaca agattatcga gaaagaaatc atgcgcgcac
     1801 cggagcagta cctctggatc caccgtcgct ttaaaacgcg cccggtggga gaatcgtcgt
     1861 tatacattta acaaactgct ctgactcttt ac
//
LOCUS       DAZKFU020000452         1887 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZKFU020000452 DAZKFU020000000
VERSION     DAZKFU020000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.454"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..881)
                     /locus_tag="ACHOQQ_RS24495"
                     /old_locus_tag="pgaptmp_004899"
     CDS             complement(<1..881)
                     /locus_tag="ACHOQQ_RS24495"
                     /old_locus_tag="pgaptmp_004899"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4473029.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEFMTGDKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYVGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPSEVRQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAAL"
     gene            complement(871..>1887)
                     /locus_tag="ACHOQQ_RS24500"
                     /old_locus_tag="pgaptmp_004900"
     CDS             complement(871..>1887)
                     /locus_tag="ACHOQQ_RS24500"
                     /old_locus_tag="pgaptmp_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4473030.1"
                     /translation="DRYGRAVAYHICEDDFPFSGSGRWERIPRELPTGRPAMLHIFEP
                     VEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESELDTEKAFEYIAG
                     APQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGDDLKLQTAQDSDNGFSALE
                     QALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIAARLATQMFS
                     CWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMRIDSGL
                     STYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIAQAYQQQIAESRRPEEETT
                     PRET"
BASE COUNT          428 a          607 c          468 g          384 t
ORIGIN      
        1 ttcagcgccg cagccatcac gctgatggca tccgacgcat tgattaattc atccgccagc
       61 ccggcctcaa taccggactg accttcaaaa acggcagcct ctgtccccgt gacagcttcc
      121 acagacagcc ccgtatacat cgccactttt tcggcaaaca tccggtgcgc cgcatcaatc
      181 cgctgctgca tgtcctggcg aacctctgac ggcaacgctt caaactgatt gccatccacc
      241 ttatgcgccc cggcataaat cagcgtgata tccactccgg cctgcgccag atgaccgaca
      301 tagctgacat ggcccatcat cacgccaatg gagccgatac gggatgtctg ggtaaccagc
      361 cgtcgggagc aggccgacgc cagcagcatg gccgcagaac aggccgtgtc attacacagt
      421 gcccagaccg gcttctgctg ccggaggcgg taaatcatgt cagcgcagtc aaacgcaccg
      481 gcggcctgcc cgcccggact gtcaatatcc agcagtacgc cccggacaga ggtgtccgcc
      541 attgcctgct gaagacaggc aacaatgccg tcatagcctg tcattccgga aaatggccgc
      601 atgccaccca gccggtgcac cagcgtgccg gtcaccggca gtacagcaat accgttcacc
      661 acccggtaaa cacgggccgg tcgtttatct ccggtcatga actcatccgt ttcagccagc
      721 attccgggag catcaagctg tacctgctgt tgtggtaccg aaagacttgc tgcccccatc
      781 tcgcgcccca gcgcgcaaaa gaaaacccgc gcataggcgg gctccagaag cagcggttca
      841 ttgaatgctg cggcaataat gtgtgaaaga ttacgtctca cgtggtgttg tctcctcttc
      901 cggcctgcgg ctctccgcta tctgctgctg atacgcctgc gctatccaca ccggacgtga
      961 gagtccggct ttttcccgct ctgcggattc cctgacctgc tggcggaaaa tgtcctgata
     1021 atcctcaccc atcagcgcca gctctttctc atacgtgctc agtccggaat caatacgcat
     1081 caccgattcc tggacctcct tgagcccgtc aatggccatt cttccggcac caatccactc
     1141 ggcccgtgac caggctgatc gcgcctgata aaaatcaaaa cgtgcccgtg gcggacggat
     1201 aatcccccga agaagtgcct cttccagcca gcaggaaaac atctgcgtgg ccagccgggc
     1261 cgcaataaat tttcgtcgtc ccataaaata gcgccacgac tcattggcag aggcgcgggc
     1321 acttgaataa ctgaccttcg agtaatcacg ggacaactgt tcgtaggaaa cgccaagacc
     1381 ggcggcgata taccgcagca gcgcctgttc aagcgccgaa aatccattgt ctgaatcctg
     1441 cgcggtctgc agtttcagat catcaccggg gaaaaggtgc ggaattttga caccgcccag
     1501 cgtcacatga ttcgtgtcat accagctgga gaacttctcc agaatattaa taagcggatt
     1561 atcctgctga ccctgcggtg cccccgcgat atattcaaag gccttttcgg tatcaagttc
     1621 actttcaatc gtcgctgcat acatggcttt aacaatggcc gactgaagct gtgttgcctg
     1681 cagggaatcg agcatcttca gccgttccat gacgctgtaa aactgattgg ccccacgggt
     1741 ctgcccgtcc tccaccggct cgaaaatatg cagcatggcc ggacgcccgg tgggaagttc
     1801 acgcgggatc cgttcccatc gtccactacc agagaacgga aaatcatcct cacagatatg
     1861 gtacgcaacg gcacggccat atcgatc
//
LOCUS       DAZKFU020000453         1887 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZKFU020000453 DAZKFU020000000
VERSION     DAZKFU020000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.455"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..881)
                     /locus_tag="ACHOQQ_RS24505"
                     /old_locus_tag="pgaptmp_004901"
     CDS             complement(<1..881)
                     /locus_tag="ACHOQQ_RS24505"
                     /old_locus_tag="pgaptmp_004901"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4473031.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSLPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPSEVRQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAAL"
     gene            complement(871..>1887)
                     /locus_tag="ACHOQQ_RS24510"
                     /old_locus_tag="pgaptmp_004902"
     CDS             complement(871..>1887)
                     /locus_tag="ACHOQQ_RS24510"
                     /old_locus_tag="pgaptmp_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4473032.1"
                     /translation="DRYGRAVAYHICEDDFPFSGSGRWERIPRELPTGRPSMLHIFEP
                     VEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESELDTEKAFEYIAG
                     APQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGDGLKLQTAQDSDNGFSALE
                     QALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIAARLATQMFS
                     CWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMRIDSGL
                     STYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIAQAYQQQIAESRRPEEETT
                     PRET"
BASE COUNT          429 a          606 c          468 g          384 t
ORIGIN      
        1 ttcagcgccg cagccatcac gctgatggca tccgacgcat tgattaattc atccgccagc
       61 ccggcctcaa taccggactg accttcaaaa acggcagcct ctgtccccgt gacagcttcc
      121 acagacagcc ccgtatacat cgccactttt tcggcaaaca tccggtgcgc tgcatcaatc
      181 cgctgctgca tgtcctggcg aacctctgac ggcaacgctt caaactgatt gccatccacc
      241 ttatgcgccc cggcataaat cagcgtgata tccactccgg cctgcgccag atgaccggca
      301 tagctgacat ggctcatcat cacgccaatg gagccgatac gggatgtctg ggtaaccagc
      361 cgtcgggagc aggccgacgc cagcagcatg gccgcagaac aggccgtgtc attacacagt
      421 gcccagaccg gcttctgctg ccggaggcgg taaatcatgt cagcgcagtc aaacgcaccg
      481 gcggcctgcc cgcccggact gtcaatgtcc agcagtacgc cccggacaga ggtgtctgcc
      541 attgcctgct gaagacaggc aacaatgccg tcatagcctg tcattccgga aaatggccgc
      601 ataccaccca gccggtgcac cagcgtgccg gtcaccggca gtaccgcaat accgttcacc
      661 acccggtaaa cacgggccgg tcgtttacct ccggccatgt actcgtccgt ttcagccagc
      721 atcccgggag catcaagctg tacctgctgt tgtggtaacg aaagacttgc tgcccccatc
      781 tcgcgcccga gcgcgcaaaa gaaaacccgc gcataggcag gctccagaag cagcggttca
      841 ttgaatgctg cggcaataat gtgtgaaaga ttacgtctca cgtggtgttg tctcctcttc
      901 cggcctgcgg ctctccgcta tctgctgctg atacgcctgc gctatccaca ccggacgtga
      961 gagtccggct ttttcccgct ctgcggattc cctgacctgc tggcggaaaa tgtcctgata
     1021 atcctcgccc atcagcgcca gctctttctc atacgtgctc agtccggaat caatacgcat
     1081 caccgattcc tggacctcct tgagcccgtc aatggccatt cttcctgcgc caatccactc
     1141 tgcccgtgac caggctgaac gcgcctgata aaaatcaaaa cgcgcccgtg gaggacgaat
     1201 aatcccccgc agaagtgcct cttccagcca gcaggaaaac atctgcgtgg ccagccgggc
     1261 cgcaataaat tttcgtcgtc ccataaaata gcgccacgac tcattggcgg aggcgcgggc
     1321 acttgaataa ctgaccttcg agtaatcacg ggacaactgt tcgtaggaaa cgccaagacc
     1381 ggcggcgata taccgcagca gcgcctgttc aagcgccgaa aatccattgt ctgaatcctg
     1441 cgcggtctga agtttcagac catcaccggg gaaaaggtgc ggaattttga caccgcccag
     1501 cgtcacatga ttcgtgtcat accagctgga gaacttctcc agaatattaa taagcggatt
     1561 atcctgctga ccctgcggtg cccccgcgat atattcaaag gccttttcgg tatcaagttc
     1621 actttcaatc gtcgctgcat acatggcttt aacaatggcc gactgaagct gtgttgcctg
     1681 cagggaatca agcatcttca gccgttccat gacactgtaa aactgattgg ccccacgggt
     1741 ctgcccgtcc tccaccggct cgaaaatatg cagcatggac ggacgcccgg tgggaagttc
     1801 acgcgggatc cgttcccatc gtccactacc agagaacgga aaatcatcct cacagatatg
     1861 gtacgcaacg gcacggccat atcgatc
//
LOCUS       DAZKFU020000454         1881 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZKFU020000454 DAZKFU020000000
VERSION     DAZKFU020000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.456"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(285..953)
                     /locus_tag="ACHOQQ_RS24515"
                     /old_locus_tag="pgaptmp_004903"
                     /pseudo
     CDS             complement(285..953)
                     /locus_tag="ACHOQQ_RS24515"
                     /old_locus_tag="pgaptmp_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
     gene            1018..1584
                     /locus_tag="ACHOQQ_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /pseudo
     CDS             1018..1584
                     /locus_tag="ACHOQQ_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
BASE COUNT          436 a          471 c          516 g          458 t
ORIGIN      
        1 attttatatc atgtggttaa gcggtggtga gttggtgagg agtgaaaagt cgaatcttcc
       61 cctttgtgat ttttgctcgt tctggatctg cccggtcagg cgatgtggtg gggggggtaa
      121 aaaatttttt taggttttac tgttcacact gttcacctct ggttttttat taataatttc
      181 atggtgatac agggtgaaga tacggtgaag ggtgaacagt ggattgttca ccttgtggca
      241 atggccagaa aggaaaagac cggctgttgc cggtctgagt gaggttatgt tgctacgggt
      301 tcgtcgcatt tcggtagcca gtcgccgtag ctttcctctt tcagcgacag attggtttgt
      361 atcccctgct tagtgtgccg cttctcataa ttcaggccgt actctttcag catcatgggc
      421 agccccagcc cgaacatttt caggctgagt acgttcctat acccgttagc ctccatatag
      481 gccagatacg cgtgatagag atatttacga taattacgcg ggatgatgct ggcattcccc
      541 ataaacatcc cgttggtctg cgggagcatt tccagatagc cgcaaaaatc aaacgtaggg
      601 tcagcatcgc gcttgatgct gagcgcctcg tcggaattct gctgtgactg gagcagtgcg
      661 caggctgcca tcgggtcgct gaacttctgc atgagctggc gcacaatcac ggccagctcg
      721 cgcgcaattt tatccctgag ctgcgggtcg cgttcctccg gcgcaatttg ctccgggaag
      781 tgaataatca cccggcgacg tgacacacca ccgctgcggt cggtgaagcg catggggtta
      841 ttgttcactg ccagaatcac cgccggaatg tgtgttgagt acgggttctg gtatttcggg
      901 tcaaccgaga ccgcatcgcc gccggtgatg gccttaagcc ctgcgccgtc accgccgccg
      961 cgtaaagtac agggctgcta cagctcgccg ttccggcatt aatcaggaga gagactcgtg
     1021 accactgtga ccatacagca ggcctttgag gcctgtcaga cgaacaaaaa tacctggctg
     1081 aaacgtaaag ccgaactggc agaccttgaa cgggaatacc gtgaacagct ccttgccggt
     1141 gacgaacaaa tcccgcgcag aatgcaggat ttgcgcgaca atatcgacgt gaaaaaatgg
     1201 gagattaatc aggccgccgg tcgttatatc cgctcacatg aggaagtgca gcacatcagc
     1261 atccgcaacc ggctccatga ctttatgcag tagcacggcg cggagctggc cgccacgctg
     1321 gcacctgagc tgatggggta taacgaacag cttcccgcag taaaacagag cgccatgcag
     1381 cactcggttg attatctgcg tgaagccctg tcggtatggc tggctgcagg tgaaaaaatt
     1441 aattattccg cgcaggacag cgatatttta acggccatcg gattcaggcc tgatgcggcc
     1501 tcgcgggatg ataatcgcca gaaattcacc ccggcacaga acctgattta cacccgccga
     1561 cgtgcagaac tggccgcacg gtagcacgca aaaaaatccg caaaattccg ctattttttc
     1621 cgaaaaaagc catgcatcca taaggtgcat ggtttttcat gcaaatcccc gtattttatt
     1681 ttcctcaccg cgccagtacc ggcgcggcct gagccggttc atgcacctgc attaaaagcg
     1741 acctcttaag cgggcaggcg tggcggggag agcattgcgc gcggagtggg tgttttattt
     1801 tttgtatgtc acataaactt aaggtatgca gtacgtagct agtttcacta atactgggac
     1861 gaaaagaatg gtgcgtttgt a
//
LOCUS       DAZKFU020000455         1857 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZKFU020000455 DAZKFU020000000
VERSION     DAZKFU020000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.457"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..509
                     /locus_tag="ACHOQQ_RS24525"
                     /old_locus_tag="pgaptmp_004905"
     CDS             <1..509
                     /locus_tag="ACHOQQ_RS24525"
                     /old_locus_tag="pgaptmp_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4473033.1"
                     /translation="SAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICR
                     GATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIRVPLTEPQKAGIASFCPY
                     NIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQISRRDQESA
                     LACWGIDR"
     gene            666..848
                     /locus_tag="ACHOQQ_RS24530"
                     /old_locus_tag="pgaptmp_004906"
     CDS             666..848
                     /locus_tag="ACHOQQ_RS24530"
                     /old_locus_tag="pgaptmp_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473034.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            863..994
                     /locus_tag="ACHOQQ_RS24535"
                     /old_locus_tag="pgaptmp_004907"
     CDS             863..994
                     /locus_tag="ACHOQQ_RS24535"
                     /old_locus_tag="pgaptmp_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473035.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            1217..1402
                     /locus_tag="ACHOQQ_RS24540"
                     /old_locus_tag="pgaptmp_004908"
     CDS             1217..1402
                     /locus_tag="ACHOQQ_RS24540"
                     /old_locus_tag="pgaptmp_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4473036.1"
                     /translation="MRELKMKLCALMLPLVVSACGSTPPVQAPCVKPPPPPAWIMQPA
                     PDWQTQLNGIISPSESG"
BASE COUNT          460 a          429 c          529 g          439 t
ORIGIN      
        1 tgtcggctgc cgttctggcg ttgattgccg caggggcttc tgcgcctgaa atcctcgacc
       61 agtttctgga tgaaaaagaa ggtaaccaca ccacggcata ccgtgatggc gcgggtatct
      121 ggaccatctg ccgtggagcc acccgggtgg atggtaagcc tgttattcct ggcatgaagc
      181 tgtcgaaggg gaaatgcgac caggttaacg ccattgagcg tgataaggcg ctggcatggg
      241 tggagaaaaa catcagagtg ccactgactg aaccccagaa agcgggtatt gcgtcattct
      301 gtccttacaa cattggcccg ggtaagtgtt tcccgtcgac gttttataaa cgaattaatg
      361 caggcgatcg caggggagcg tgtgaagcga ttcgctggtg gattaaggac ggtggcagag
      421 actgccgtat ccgctcaaac aactgttatg gtcagatatc ccgtcgtgac caggagagcg
      481 cgctggcgtg ctggggaatc gacagataag cagaatattt tgctgaaaaa tgacgttggc
      541 caacgcggac ggataacacg aaatcctgcg aactggcaaa acctaagtga ataaaagtaa
      601 aaaccccgtt tgttggcagc aagcggggtt ttgtgtttct gaccttggat aaggcaaggg
      661 agaacatgga aaagtataaa cgaattctgt tgaggttgac tatgaaaaac ggccttgaac
      721 tgaaagcgcc agtaactgat gacatcagca gagcactggc ttttgctatt aagtgggtgg
      781 cggtcggtat cgctgtgtct ccgatgctgt atgggctggc aaaactggtc attgcgttga
      841 aatcgtgaag ggaggattaa gcatgtcaga caaactcata atgctggcga agatcctctg
      901 tgtaatcgtc ggtatttcat tttcactaat gctggttgct atttttcttt tcctgccctg
      961 gatgatgttg tcttcgtcgg ggatgctggg gtgagcataa accgaatgct ttccgcgttt
     1021 accgttattc tgctggtggc ctgtggtgcg cttagtctgg ggctgaatca ttaccgcgat
     1081 aacgccatca cctacaaagc gcagcgcgat aaaaaagcca gagagctgga acaggcgaat
     1141 gccaccatta ctgacatgca ggtgcgccag cgtgatgttg ctgcgctcga tgcaaaatac
     1201 tcgagggaat tagccgatgc gagagctgaa aatgaaactc tgcgcgctga tgttgccgct
     1261 ggtcgtaagc gcctgcggat caacgccacc tgtccaggct ccgtgcgtga agcccccacc
     1321 acctccggcg tggataatgc aaccggcccc cgactggcag acacagttga acgggattat
     1381 ttcaccctca gagagcggtt gatgatgatg cagaagcagc tggaaggggc gcaggaatat
     1441 atccgcactc agtgcctgaa ataagttttg ctgatgcgcc gtatcgtcgc cgtattcccg
     1501 tattaacaga gaccgcagcc cgacagggag actcctctgc gagagtgtgc ggggataatc
     1561 aaaaacgata cacaccgggg tttaccgcgt taacagagcg cggcgttgtc ccctcatagt
     1621 cgcctgtccg gtgcgatggt ggaagaaact ggatttgttg caactgataa ccattatcat
     1681 tttctcgggt cctttccggc atatggaccc gttacggggc ggcgacctcg cgggttttca
     1741 ctatttatga aaattttccg ggatccatgt ccggtttctc tgcaagttaa ctatatgaaa
     1801 aatataaaaa caggtcttct gtgaaccgga catgcgcaaa aaatgggcat gtgaacc
//
LOCUS       DAZKFU020000456         1854 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZKFU020000456 DAZKFU020000000
VERSION     DAZKFU020000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.458"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..528
                     /locus_tag="ACHOQQ_RS24545"
                     /old_locus_tag="pgaptmp_004909"
     CDS             <1..528
                     /locus_tag="ACHOQQ_RS24545"
                     /old_locus_tag="pgaptmp_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4473037.1"
                     /translation="EEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLG
                     REGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLY
                     DLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDH
                     PKLQGIAQQNSFKHT"
     gene            898..1722
                     /gene="gadX"
                     /locus_tag="ACHOQQ_RS24550"
                     /old_locus_tag="pgaptmp_004910"
     CDS             898..1722
                     /gene="gadX"
                     /locus_tag="ACHOQQ_RS24550"
                     /old_locus_tag="pgaptmp_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGU4473038.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMKRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQRLPNRDSAASIVAQGNFYGADCSAEGIRL"
BASE COUNT          513 a          391 c          409 g          541 t
ORIGIN      
        1 gaagaagcgc tgccgcagga actggtgttc aacgttgact acctcggtgg tcagattggt
       61 actttcgcca tcaacttctc ccgcccggcg ggtcaggtaa ttgcacagta ctatgaattc
      121 ctgcgcctcg gtcgtgaagg ctataccaaa gtacagaacg cctcttacca ggttgccgct
      181 tatctggcgg atgaaatcgc caaactgggg ccgtatgagt tcatctgtac cggtcgcccg
      241 gacgaaggca ttccggcggt ttgcttcaaa ctcaaagatg gtgaagatcc gggatacacc
      301 ctgtacgacc tctctgaacg tctgcgtctg cgcggctggc aggttccggc cttcactctc
      361 ggcggtgaag ccaccgacat cgtggtgatg cgcattatgt gtcgtcgtgg cttcgaaatg
      421 gactttgctg aactgttgct ggaagactac aaagcctccc tgaaatatct cagcgatcac
      481 ccgaaactgc agggtattgc ccagcagaac agctttaaac acacctgata acataacgtt
      541 gtaaaaaccg aatgcccagc ctttaaaaaa acagctgggc attcggttgc ttattaatcg
      601 caataatata ttggctgtct attcatcgtg ttaataagat atatgctata tgaatgttta
      661 ttaaatgtag cgattacctt taatgtcacg aaaagatatt atttgcttac tattaatttc
      721 cctgcgttct taaacacgtt ttttgtatta taaatccagt catcctgccc gtagaacatc
      781 acacattatc atcctgttct cccgcttcgt ttaaattttt tatcaatcaa tttgacttaa
      841 gagggcggcg tgctacatta ataaacagta atatgtttat gtaatattaa gtcaactatg
      901 caatcactac atgggaattg tttaattgcg tacgcaagac ataaatatat tctcaccatg
      961 gttaatggtg aatatcgcta ttttaatggc ggtgacctgg tttttgcgga tgcaagccaa
     1021 attcgagtag ataagtgtgt tgaaaatttt gtattagtat caagggatac gctttcatta
     1081 tttctcccga tgctcaagga ggaggcatta aatcttcatg cacataaaaa agtttcttca
     1141 ttactcgttc atcactgtag cagagatatt cctgtttttc aggaagttgc gcaactatcg
     1201 cagaataaga atcttcgcta tgcagaaatg ctacgtaaaa gagcattaat ctttgcgttg
     1261 ttatctgttt ttcttgagga tgagcacttt ataccgctgc ttctgaacgt tttacaaccg
     1321 aacatgcgaa cacgagtttg tacggttatc aataataata tcgcccatga gtggacacta
     1381 gcccgaatcg ccagcgagct gttgatgagt ccaagtctgt taaagaaaaa attgcgcgaa
     1441 gaagagacat catattcaca gttgcttact gagtgtagaa tgaaacgtgc tttgcaactt
     1501 attgttatac atggtttttc aattaagcga gttgcagtat cctgtggata tcacagcgtg
     1561 tcgtatttca tttacgtctt tcgaaattat tatgggatga cgcccacaga gtatcaggag
     1621 cgatcggcgc agagattgcc gaaccgtgac tcggcggcaa gtattgttgc gcaagggaat
     1681 ttttacggcg ctgactgttc tgcggaagga ataagattat agagttttac tcagacataa
     1741 aaaaaacccg gcatagggga ccgggaagag gatagtctgc cgtctccaga ctaataaacc
     1801 gttataacac tccctgttgg cacgggaaac tttgtgctct cagtaagtta aata
//
LOCUS       DAZKFU020000457         1851 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZKFU020000457 DAZKFU020000000
VERSION     DAZKFU020000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.459"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..1628
                     /gene="lysS"
                     /locus_tag="ACHOQQ_RS24555"
                     /old_locus_tag="pgaptmp_004911"
     CDS             111..1628
                     /gene="lysS"
                     /locus_tag="ACHOQQ_RS24555"
                     /old_locus_tag="pgaptmp_004911"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4473039.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGDRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(1747..>1851)
                     /gene="ghoT"
                     /locus_tag="ACHOQQ_RS24560"
                     /old_locus_tag="pgaptmp_004912"
     CDS             complement(1747..>1851)
                     /gene="ghoT"
                     /locus_tag="ACHOQQ_RS24560"
                     /old_locus_tag="pgaptmp_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGU4473040.1"
                     /translation="FISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
BASE COUNT          509 a          411 c          452 g          479 t
ORIGIN      
        1 ttagtttcga taagataaac tgagttatta atagtcgagg cagataatac agtgtaccga
       61 tctgtctctt tatctacgct aaattgaaag ctggatttag aggaaccaaa atgtctgaac
      121 aagaaacacg gggagccaat gaggctattg attttaacga tgaactgaga aatcgccgcg
      181 aaaaactggc ggcactacgt cagcaaggtg tggcgtttcc caatgatttt cgccgcgacc
      241 atacctctga ccagctgcac gaagagtttg atgcgaagga taaccaggaa ctggaatcct
      301 taaacattga agtctcggtt gctggccgaa tgatgacccg tcgtatcatg gggaaagcct
      361 cctttgtaac gttgcaggat gtcggtgacc gtattcaact gtacgttgca agagatagcc
      421 tgccagaagg tgtttataac gatcagttta aaaaatggga tctgggtgac attatcggtg
      481 cccgcggtac gctgtttaag acgcaaaccg gtgagctttc cattcactgt actgagttgc
      541 gcctgctgac aaaagcacta cgtcctttac cagataaatt ccatggtctg caggatcagg
      601 aagtccgtta tcgtcaacgt tatctggacc tcatcgctaa cgataaatcc cgtcaaacgt
      661 ttgttgtccg ttcaaaaatt ctggccgcta tccgccagtt catggtcgcg cgcggcttta
      721 tggaagtaga aaccccgatg atgcaggtaa ttccaggtgg ggcatctgct cgcccgttta
      781 ttacccatca taacgctctg gatttagata tgtacctgcg tatcgcgccg gagctgtatc
      841 tgaaacgtct ggttgtaggc ggttttgaac gggtattcga aatcaaccgt aacttccgta
      901 atgaaggtat ttctgttcgc cataatcctg agttcacaat gatggaactc tacatggcgt
      961 atgcggatta ccacgatttg attgaactga cagagtccct gttccgcacc ctggcacaag
     1021 aggttctggg taccactaaa gtcacttatg gcgagcatgt gtttgatttc ggcaaaccgt
     1081 ttgaaaaact caccatgcgc gaagcaatca aaaaatatcg tccagaaacc gatatggccg
     1141 acctggataa ttttgatgct gctaaagcat tagctgaatc tatcggaatt acggtagaga
     1201 aaagctgggg gctgggacgt attgtcacgg agatctttga tgaagtggca gaagcacatc
     1261 tgattcagcc aacctttatt acggaatatc cggcagaagt gtcaccgctg gcgcgccgta
     1321 atgatgttaa cccggaaatc accgaccgtt ttgaattctt catcggtggt cgtgaaatcg
     1381 gtaatggttt tagcgaatta aacgacgcag aagatcaggc tgaacgtttc caggaacagg
     1441 ttaacgctaa agctgcaggt gacgacgaag ccatgttcta tgacgaagat tacgtgactg
     1501 cgctggaata tggtctgccg ccaaccgctg gtctgggtat tggtatcgac cgaatgatta
     1561 tgctgtttac aaacagtcat actattcgcg acgttattct cttcccggcg atgcgcccac
     1621 agaaataaat ttcactttaa tgaacgaagc agtcaggcga ctgcttcgtt cgtcgttccg
     1681 caaatccagt tttgaggaga gacgcttcgt ttcacgataa ccagcgggtc cactcaacta
     1741 cagctcctaa aagagagaaa aaagtaatgc cacaggcaga ctcattggcc aggttattcc
     1801 gaccaggaat gcactaagta aacgaatatg tgttttctca tgtgagataa a
//
LOCUS       DAZKFU020000458         1848 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZKFU020000458 DAZKFU020000000
VERSION     DAZKFU020000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.460"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..833
                     /locus_tag="ACHOQQ_RS24565"
                     /old_locus_tag="pgaptmp_004913"
     CDS             <1..833
                     /locus_tag="ACHOQQ_RS24565"
                     /old_locus_tag="pgaptmp_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4473041.1"
                     /translation="SAEETKQLEEVKKERADMFATYMATHDAVSVGVGATTTGRHAVT
                     RQITSVAAGSVDTDAVNVAQLRDLQTVTEKADAALDKRITNNTSAITKNLQVVNSNKE
                     EISNINKTTKILDERVTANTQTAEEHKLLISENSKQISTNTLGIRENTQRIRDEGQAR
                     IRGDRETLRAATGYTDMRVNGLERNTNKRFAQLKEDIDKNRKRADAGIAGAMAMTGIP
                     SVPGKKVSFGMAMAGYRSEGAVAAGFHFNTSENSAVKVNAAWDTENGAGVSAGMALGW
                     "
     gene            complement(1263..1838)
                     /gene="dolP"
                     /locus_tag="ACHOQQ_RS24570"
                     /old_locus_tag="pgaptmp_004914"
     CDS             complement(1263..1838)
                     /gene="dolP"
                     /locus_tag="ACHOQQ_RS24570"
                     /old_locus_tag="pgaptmp_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU4473042.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRLSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADVASRVSGVKRVTTAFSCIR"
BASE COUNT          524 a          443 c          466 g          415 t
ORIGIN      
        1 tgtctgctga agagactaaa caactggagg aagtgaaaaa agagcgggct gatatgtttg
       61 cgacatatat ggcaacgcac gatgctgtat ctgtaggtgt tggtgcaacg acaacaggac
      121 gacatgctgt tacaagacag ataactagcg ttgcagcagg ttctgtcgat acggatgctg
      181 ttaacgttgc acagctcagg gatttgcaga ctgtgacaga aaaggcagat gcagcgcttg
      241 ataaacgcat aacaaacaac accagtgcga taactaaaaa tctgcaggtg gttaacagta
      301 ataaagagga aatcagtaac attaataaaa cgacaaaaat tctggatgaa agagtgacag
      361 ccaatacaca gacagcagaa gaacataagc tgcttatttc ggagaacagc aaacaaatca
      421 gtaccaacac tcttggtatc agagagaata cacaacggat ccgggatgaa ggacaggctc
      481 gaatccgggg agacagggaa acactgcgtg cagcgaccgg ttataccgac atgcgggtaa
      541 acgggctgga gcgtaatacg aataaacgtt ttgcgcagct taaagaggat atagacaaaa
      601 acagaaaacg tgcagatgca ggtattgcag gtgcgatggc aatgacgggg ataccgtccg
      661 tacccgggaa aaaggtctca tttggtatgg ccatggccgg ataccggagt gaaggtgctg
      721 ttgctgccgg cttccatttt aatacaagtg aaaacagtgc tgttaaagtg aatgcagcct
      781 gggacactga aaatggagct ggcgtttcag ccgggatggc tctgggatgg taatgcttcc
      841 gggccagaag ccgttcgcag atattgttat ccaggggaca ctgcaccacc attcaggaag
      901 cgattcagtt tatcgcatcg gctcaggatg taattaatac ggacaatctg gctcctgcac
      961 cggagccaga cgtacacgtg tcaacgccac gctctttgac ctgtatggat aaaagctcag
     1021 attatttacc agcagcgaca tccggagcgc accgggattt tatctgtgag tgaacatacc
     1081 cgggcaaaaa aatcccccga attcaggggg gtgtcaaaga ggaattatgt atattggtta
     1141 ttataatgag cacgcctccg gtattccgcg acgaatgttc acgtggtggc atgaacggcc
     1201 cggaaacgtg ctcttctgat ttttaacgta aatttacact aaaggcaact ctgatactgc
     1261 cttcaccgta tacaggagaa ggccgtcgtg acccgcttca ctccgctcac cctactggca
     1321 acatctgctg ccgctcgccc ttcctgctct gtaaccaggc ccagtaaaaa gacttcgctg
     1381 ttttctgtca ccaccttgac actggatgat ttgacacggg cacttgtgag taactgagaa
     1441 cgaatttttg tcgtaagcca ggcatcacct gccattatga ccggactgac gggtgtcccc
     1501 tgccggattt cattatagac ctcctgaaca ccatcgacac cgagtgctat ctgacgggca
     1561 cgggcagata attcacggtc cggagactga ccgatcagca gcacgcgtcc ctgatatgca
     1621 atctcactga tgcgtgcttc ttttctgatt tgttcatctt ttgaaagtgc actgctgagc
     1681 ctcagctcca gcacttcatc atccacctgc gttccgacac tgcgtgggtc ggttgctgcc
     1741 tttgtcccga cagcggctgt acccaccaca gttgcagcaa cgcatccctg taacaataag
     1801 gctgaaatca ggacaacagc agaatataat aaacgcatat ctcccccg
//
LOCUS       DAZKFU020000459         1847 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZKFU020000459 DAZKFU020000000
VERSION     DAZKFU020000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.461"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..1738
                     /gene="yfbK"
                     /locus_tag="ACHOQQ_RS24575"
                     /old_locus_tag="pgaptmp_004915"
     CDS             11..1738
                     /gene="yfbK"
                     /locus_tag="ACHOQQ_RS24575"
                     /old_locus_tag="pgaptmp_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HGU4473043.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPQPENKESQQQQPSTPTEQQVLAA
                     QQAAIKEAEQSAAAAKALAQQEVQQYSDKQALQGRLQEAPTFARAAKAKATHIANPGT
                     ARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEIVNYF
                     PSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELPASNLV
                     FLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAE
                     INAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIE
                     LMLKKQRESGVTLSTFGVGDDNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQ
                     TLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLF
                     ELTLNGQKASIDKLRYAPDNKLAKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTIN
                     APSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGVDPQGYRAEFIRLIEL
                     ADGVTDISQ"
BASE COUNT          536 a          392 c          468 g          451 t
ORIGIN      
        1 aggattttat atgcgaaata aaaatataat catgttgctt atgagtagtt tgattttgtc
       61 aggatgtggg ccgcaacctg agaataagga aagtcagcaa caacaaccca gtactcccac
      121 agagcagcaa gtgcttgccg cgcagcaagc tgcaataaaa gaggctgagc aaagcgccgc
      181 cgccgcgaaa gccttggccc agcaagaagt gcaacaatat tcagacaaac aggctttaca
      241 ggggcgattg caggaagcgc caacatttgc aagagcggct aaagcaaaag ctacacatat
      301 cgcaaatcca ggaaccgctc gctaccagca gttcgatgat aatccggtta agcaggtagc
      361 gcaaaatccg ttggcgacgt ttagtcttga cgttgacact ggcagttatg cgaatgtaag
      421 gcgtttcctc aatcaagggc tgttacctcc gccagacgct gtgcgggtgg aggagatagt
      481 caattatttc ccgtctgatt gggatatcaa agacaaacaa tctattccgg cctctaagcc
      541 aatacctttc gctatgcgct acgaattggc acctgcacca tggaatgaac agcgaacatt
      601 gctgaaagtt gatatcctgg cgaaagatcg caaaagtgaa gagttaccag cttctaatct
      661 ggtctttctt atcgacactt ctggttcaat gatttctgat gaacgtttgc cacttatcca
      721 gtcttcgttg aaattattgg tcaaagaact tcgtgagcag gataacattg ccatcgtgac
      781 ctacgctggc gactcccgta ttgcattgcc ttctatctcc gggagtcata aggcggaaat
      841 taatgccgca attgattcgc tggatgccga aggcagtacc aatggcggtg ccgggctgga
      901 actggcttat cagcaggcgg cgaaggggtt tattaagggc ggtatcaatc gcattttatt
      961 ggccactgac ggtgacttta acgttggtat tgacgatcca aaatcgattg aattaatgct
     1021 caaaaaacag cgggagtccg gtgttactct gtcgacgttt ggcgtggggg atgacaatta
     1081 caacgaggca atgatggtgc gaattgccga tgttggcaac ggtaactaca gctacattga
     1141 taccctctct gaagcgcaga aagtattgaa cagtgaaatg cggcagacgt tgattaccgt
     1201 agcaaaagat gtcaaagcgc aaattgagtt taaccccgcg tgggtaacgg aataccgtca
     1261 gattggttat gaaaagcgcc aacttcgggc ggaagatttt aataacgaca acgttgatgc
     1321 gggggatatt ggcgcaggca aacatataac gttgttattt gaattaacgc tgaacgggca
     1381 aaaagcatca attgataagt tacgctatgc cccggataac aaattagcga aatcggacaa
     1441 aacgaaagaa ctggcctggt taaaaattcg ctggaaatac ccgcagggaa aagaaagtca
     1501 gttagttgaa ttcccgctgg ggccaacaat aaacgcgccc tctgaagata tgcgttttcg
     1561 cgcagcagta gctgcatatg ggcaaaagtt acgcggttct gaatacctga acaatacctc
     1621 ctggcagcag atcaaacagt gggctcagca ggcaaaaggg gtagatccac agggttacag
     1681 ggcggaattt attcgcctga ttgaactggc ggatggtgtg actgacatca gtcagtgatg
     1741 actttttagc aaactatgtt cgaccagtca gcatatttgc tgactggtcg aattaattaa
     1801 caatgatgtt aacttactct tttgcttgat gctctatttt tgggttg
//
LOCUS       DAZKFU020000460         1822 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZKFU020000460 DAZKFU020000000
VERSION     DAZKFU020000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.462"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1822
                     /locus_tag="ACHOQQ_RS24580"
                     /old_locus_tag="pgaptmp_004916"
     CDS             <1..>1822
                     /locus_tag="ACHOQQ_RS24580"
                     /old_locus_tag="pgaptmp_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473044.1"
                     /translation="KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAF
                     GCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGR
                     ILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPVRVDITAHPAPDRIQVSVLPDG
                     VATYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPMSG
                     SLNSVIPPAVQHLTVEVSASDGQYLAQAKWDTPRVVKGVRFSLRLTSGSGENSRLVTS
                     AITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGYYQ
                     ITAVPVLAVYDPTVQYEFWFSEKRITDMAQVETFAHYLGTGSQWSVSGPHIKPGKDFW
                     FYVRSVNLVGKSVFVEASGRASNDAEGYLGLFREKIGKLHLAQGMWELIDNSQLDDEM
                     AEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGI
                     PYVAGIGAGIEDADGRPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKR
                     LFAVSITSSGNPPTFSLTPEGRLTARNADISGHISANSGTL"
BASE COUNT          421 a          506 c          571 g          324 t
ORIGIN      
        1 tgaaggaccg gcacacggcg gtggaggtga attacaccga cccgcagaac ggctggcaga
       61 cctccacgga actggtggaa gaccctgaag ccatactgcg ctacggacgc aacctgctga
      121 agatggatgc gttcggctgt accagccgcg gtcaggccca ccgtgccgga ctgtgggtga
      181 taaagaccga actgctggaa acgcagacgg tggatttcac gctcgggtct caggggctgc
      241 ggcacacacc cggtgacatc attgaaatct gtgataacga ctatgccggg accctgaccg
      301 gcggacgcat cctgtccatc gatgccgcca gccgcacact gacgctggac cgtgaggtga
      361 ccctgccgga gacaggtaca tcgacggtga acctgattaa cggcagcggt aagccggtgc
      421 gcgtggacat cactgcacac cccgccccgg accggataca ggtcagcgtc ctgcctgatg
      481 gcgtggcgac atacggtgtg tggggactct ccctgccgtc actgcgtcgt cgcctgttcc
      541 gctgtgtttc catccgggaa aacacggacg gcacctttgc catcacggcg gtgcagcatg
      601 tgccggaaaa agaagctatt gtggataacg gggcccgctt tgagccgatg tccggctcac
      661 tgaacagcgt catcccgccg gcagtgcagc acctcacggt ggaggtgagt gcctcagacg
      721 gccagtatct ggcacaggcg aaatgggaca cgccgcgggt ggtgaagggc gtgcgcttca
      781 gtctgcgcct gaccagtggc agtggtgaaa acagccgcct ggtgaccagc gccatcactg
      841 ccgacacgga gcaccgtttc agtggcctgc cgcccgggga atacaccctg acggtcaggg
      901 cgataaacag ctacggccag cagggcgaac ctgccaccac tacattccgg attaatgcgc
      961 ctgcagcacc ggccagcatt gagctgacgc cggggtatta tcagataacg gcagtaccgg
     1021 tgctggcggt gtatgacccg acggtacagt atgaattctg gttctcagaa aaacgcatca
     1081 cggacatggc acaggtggaa acctttgccc attatctggg gacaggcagc cagtggagtg
     1141 tctccggccc gcacattaag ccggggaagg atttctggtt ttatgtgcgc agcgtcaacc
     1201 tggtggggaa atctgtgttt gtggaagcca gtggccgggc cagcaatgat gcagaagggt
     1261 atctggggct gtttcgggaa aagataggaa aactgcatct ggcacagggg atgtgggagc
     1321 tgattgataa cagccagctt gatgatgaga tggcagagat gaagaccacc atcacagaaa
     1381 cccgcaatga aatcacacag acggtcagta aaaccctgga agaccagagt gccaccatac
     1441 agcagataca gcgggtgcag acagacacaa ataacgacct ggctgcgctg tacatgctga
     1501 aggtgcagaa aacgaaaaac ggcattccgt atgttgccgg tataggtgcg gggattgagg
     1561 atgctgatgg ccggcccctg agcaatatac tgctgcaggc ggaccgtatc gcgatgatta
     1621 acccggagaa cggcaacacc acgccgctgt ttgtggcgca ggggaatcag ctgttcatga
     1681 acgatgtgtt cctgaagcga ctgtttgcag tgagcatcac ctcgtccggc aatcccccga
     1741 cgttctccct gacgccggag ggcaggctga cggcccgcaa tgctgacatc agcggacata
     1801 tcagtgcgaa ctcgggcacg ct
//
LOCUS       DAZKFU020000461         1818 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZKFU020000461 DAZKFU020000000
VERSION     DAZKFU020000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.463"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            136..639
                     /locus_tag="ACHOQQ_RS24585"
                     /old_locus_tag="pgaptmp_004917"
     CDS             136..639
                     /locus_tag="ACHOQQ_RS24585"
                     /old_locus_tag="pgaptmp_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGU4473045.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(718..969)
                     /gene="yecJ"
                     /locus_tag="ACHOQQ_RS24590"
                     /old_locus_tag="pgaptmp_004918"
     CDS             complement(718..969)
                     /gene="yecJ"
                     /locus_tag="ACHOQQ_RS24590"
                     /old_locus_tag="pgaptmp_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGU4473046.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1084..1170)
                     /gene="azuC"
                     /locus_tag="ACHOQQ_RS24595"
                     /old_locus_tag="pgaptmp_004919"
     CDS             complement(1084..1170)
                     /gene="azuC"
                     /locus_tag="ACHOQQ_RS24595"
                     /old_locus_tag="pgaptmp_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGU4473047.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1433..1756
                     /gene="yecR"
                     /locus_tag="ACHOQQ_RS24600"
                     /old_locus_tag="pgaptmp_004920"
     CDS             1433..1756
                     /gene="yecR"
                     /locus_tag="ACHOQQ_RS24600"
                     /old_locus_tag="pgaptmp_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HGU4473048.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          466 a          430 c          410 g          512 t
ORIGIN      
        1 ttatttcaga tcatcgtcag aattgactcc acgatcacat ttcggaccgg cagaaaggaa
       61 ttattctgca aacagtaatt atggtgtttt gatttatctt gcacctctcc acttctggat
      121 ataaggatat taggtatggc aaccgctgga atgcttctca aactcaactc tcaaatgaac
      181 cgcgagtttt acgcatccaa tctctacctt cacctgagta actggtgttc tgaacagagt
      241 ctgaacggca ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg
      301 cgcatgttta actttatgaa gagtgtcggc gctaccccca tcgttaaagc cattgatgtt
      361 cccggtgaaa aactgaactc tctggaagaa ctgttccaaa aaacgatgga agaatacgag
      421 caacgttcta gtacgttggc acagttagcc gatgaagcga aagaactgaa tgatgattca
      481 accgtcaatt tcctgcgcga tctggaaaaa gaacagcagc atgatggcct gttgctgcaa
      541 accattcttg atgaagtgcg cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac
      601 caacatgttc tgaatgtcgt gtcgcatcag ctgcattgat catacatcgg cgctaatgca
      661 ttgcgccgat gaagtttttg agaaaccgct gcctcatctt tttgaagcag cggtttttta
      721 atgggattcg ccttgtgggg taaacttgag ttcaatcagc gcgatggctt tttggattgc
      781 ccgcatggtg accgggtctg cggcggcggg atgatgagta aagtcgatat tcttcagctg
      841 gctggacatt ttttcgcgaa cttcaacggg cgcgattaca tcgagaacat ccagaatttg
      901 tttgataacc aactggcaag caaccacatc agaaaccagt tcctgatcgg cattcagcgg
      961 ctgggacatc gtaaactcct gatagcattt tgaaagccgt tatagtagcg gcttcacatc
     1021 ttcagcgata gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt
     1081 ttgttatcgg aacatattgc ctggcggtac gtctttgaac gtcttgcaat agttattgaa
     1141 catacttttc aggattttgc gcagtttcat cgcggcactc cgaccatttg ttatacaggt
     1201 gttattgtct ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt tatgtgatgc
     1261 aaatcacata aatacccctt taatgttata aaaatgataa ccaaaaaaca gcccccctat
     1321 ttctgacacc tacagatggc aggaaatagc gcctgccagg cgtcttttcc ggccattgtc
     1381 gcagcgctgt aacgcgtaaa atagtgcttt ctcttactct tctggctgga ccatgagact
     1441 tctgattctg actctttcat taataacgct ggcggggtgt acggttactc gccaggccca
     1501 tgtgagcgaa gttgacgcgg caacaggtat tgtacggttg gtttatgacc aggctttttt
     1561 gcagcatgcc cataccgatc gttatgtcag tcgcgggatc gccgatcgcg catgccagca
     1621 ggaaggctat acccacgcga tcccctttgg tcagccagta ggcaactgta gcctttttgc
     1681 gggttcctta tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt
     1741 ccctgtcttt ctctaaatat tttaatcagc gaggggatct tcgctgatta aagaaatagc
     1801 ggtaatgcgt tttattcc
//
LOCUS       DAZKFU020000462         1806 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZKFU020000462 DAZKFU020000000
VERSION     DAZKFU020000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.464"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(580..>1806)
                     /locus_tag="ACHOQQ_RS24605"
                     /old_locus_tag="pgaptmp_004921"
     CDS             complement(580..>1806)
                     /locus_tag="ACHOQQ_RS24605"
                     /old_locus_tag="pgaptmp_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HGU4473049.1"
                     /translation="RVRQLTSAVLVCKCGALGCSVFEGNIADDWSQVQMHSGVRVDIL
                     NVLGAGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPTKQELDDYLS
                     REHAITRPDKDPHLNHLHRTTTRKQHWPELCVFAFDHRNQLVDIARATGASEFTIPEL
                     KMLLLEGARQAAHEAGLLHNCGILADTTFGQKALNDVTGQGWWIGRPIEMPGSYPLKL
                     EHGNIGSQLVSWPKEHVVKCLVFYHPLDAESLRLKQEALIDEIYRACCQSGHDLLLEV
                     ILPRDAADQSEHYYTQIVERFYLLGIQPDWWKLPPLSPARWQEISQHIERYDPECRGI
                     LILGLDAPESELKSGFAAAADIPWVKGFAVGRTIFGEPSRQWFSGQINDEQLIAAVKQ
                     KYRTLIDYWREYRPAL"
BASE COUNT          453 a          463 c          470 g          420 t
ORIGIN      
        1 atatccgtca tggctctgca aaaaactgta aaatgaagcc gaatgtacgg gggcagtctg
       61 aaccccaact gaagcatctt ttttgcacaa aatcgggagt ccatgtacgg atataaagct
      121 gcaacctacc gtcaatgtca gaacaatgga agctgtgaaa aacgggatgc gttaatataa
      181 actccatgac agtagtggaa cgggttaatc aagcaggatt accagcaaga tgagggagct
      241 gattcagcac aggcgacctg accctggcca cggataaccg tctgtcgccc gtctgaggtg
      301 tcgttttttc gttctgccac ccctcggttg agtaaaatgg aaattaaccg tcaagctgcc
      361 tgtagcgtac cgatcagaag attgctcaga tggtacagct gttgtacgtt ctgcggcaac
      421 gtgacgaggg aactaccaat agccagacag ttcagaaata tcattaatgc agatacgaaa
      481 cagcactgtc gggatgatga ggaaagtagg acggtcatga aacatctttg aatacgcccc
      541 gagcaagaat acagacgcgg gacgtaatgc agacggatac taaagcgccg gacgatattc
      601 gcgccagtag tcgatcaatg tgcggtattt ttgtttcact gctgcgatga gctgttcatc
      661 gttaatctgg ccgctgaacc actgccgtga tggctcgcca aagatggtgc gacctacggc
      721 gaagccttta acccatggta tatccgcagc ggcggcaaag ccagatttca gctcgctttc
      781 cggtgcatcc agtccaagta ttaaaatacc gcggcattcg ggatcgtagc gctcaatatg
      841 ctggctgatt tcttgccaac gagccggact taacggtgga agcttccacc agtcaggctg
      901 gatccccagc aggtaaaaac gttccacaat ctgtgtatag taatgctcgc tttgatctgc
      961 tgcgtcgcgt ggcaagatga cctccagcag cagatcgtgt ccagactggc agcaggcccg
     1021 gtatatctca tcaatgagtg cttcttgctt aaggcggagg gattctgcat ccagtgggtg
     1081 atagaagaca aggcatttca cgacgtgctc tttcggccaa ctgacaagtt gtgaaccgat
     1141 attaccgtgc tccagcttca gaggatatga ccccggcatt tcgataggac gtccaatcca
     1201 ccatccctga ccagtcacgt cgttaagtgc tttctgacca aaagtagtat ccgccaggat
     1261 gccgcagtta tgcagtaatc cagcctcgtg cgccgcctga cgtgcgcctt ccagtagtag
     1321 catcttcagc tcagggatag tgaactcgct ggcacctgtc gctctggcga tatccaccag
     1381 ttgattgcga tggtcaaagg cgaatacaca cagttccggc cagtgctgtt ttcgggttgt
     1441 cgttcggtga agatggttaa ggtgagggtc tttgtcgggt ctggtgatag cgtgctcacg
     1501 ggagagatag tcatccagtt cctgtttggt tggcatcgcg ggggcgcagc cgtggcgcga
     1561 tacaactaat gcgccacatg cgttagcata acgacaagct tgctcccaac cttcatcgtt
     1621 cagatagcct cgaagcagtc ccgacataaa ggcgtctcct gcacccagca catttaggat
     1681 gtccacacgt acaccgctgt gcatctgcac ctgagaccag tcgtcggcga tgtttccttc
     1741 aaatactgaa caccctaatg cgccgcattt gcataccaat accgcagagg ttagctgacg
     1801 cacacg
//
LOCUS       DAZKFU020000463         1806 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZKFU020000463 DAZKFU020000000
VERSION     DAZKFU020000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.465"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..191)
                     /locus_tag="ACHOQQ_RS24610"
                     /old_locus_tag="pgaptmp_004922"
     CDS             complement(<1..191)
                     /locus_tag="ACHOQQ_RS24610"
                     /old_locus_tag="pgaptmp_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473050.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSE"
     gene            complement(280..903)
                     /locus_tag="ACHOQQ_RS24615"
                     /old_locus_tag="pgaptmp_004923"
     CDS             complement(280..903)
                     /locus_tag="ACHOQQ_RS24615"
                     /old_locus_tag="pgaptmp_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4473051.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYYL"
     gene            complement(992..1501)
                     /locus_tag="ACHOQQ_RS24620"
                     /old_locus_tag="pgaptmp_004924"
     CDS             complement(992..1501)
                     /locus_tag="ACHOQQ_RS24620"
                     /old_locus_tag="pgaptmp_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473052.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
BASE COUNT          486 a          487 c          389 g          444 t
ORIGIN      
        1 tgttcactgt atgccgtgtt cagcgcatcc caggaaacgt tgatataaaa gctgtcctgc
       61 aatttgcggg taacaatttc tgccacccgg ttacggttat ccgcctgtcc ggcataacct
      121 ctctctgcca gccactcatt cagttcatgt tccggagaca gattgataat atcgtaatcg
      181 gaccttgcca ttcccgtttc cttagcacat gttttcaggc tcggggataa agaatgtcgt
      241 actgctgctg ccagagcaat attccccctg atggtcgaat cagagatagt aggccggtac
      301 tttgcgggtt gttcccgtca gattatcatg cacctccgcg gtcgttgtca ccaccggcat
      361 tccgctcttt ctcaacctca ggacatcagc cgcaatacgc tgactgccga accagaacag
      421 gccgtccagt gcagtcacca gcaaccccgc ctccagcgca tgcttcagcc gttcacgggg
      481 cgctttcact tcccgggcaa tctgctggta tggcgatgat gtgttttcat tcccaatcac
      541 ccggcgaata cgatgagaca gatgataccg gtatgtatcc ggcacaccgg aaaggctggc
      601 cttcaggctg tacacgcagc caaagcgttt atcgttgaac accacatttt tctggctgat
      661 atcccattct tcacgcagcg cggcaatcag ttgtggtgtc cgggtaagca acaagcgaaa
      721 aggcagttca aaactggtga cgtaatccac attcagcagt gcaatacgca gccgttcttc
      781 cggcccggcc tctgtctgcc ggcactcctc cagaacatcc tgccactgct ggtgaaggcg
      841 tggaagttca ttcagttcag taaaactgta tttatccgca agatagtcaa ttcgtgtacg
      901 catactgaaa agaattccgt ataaagattc agctggcaaa actttatcag tctgtaaaaa
      961 ctaacggaag agtcgatatt tctcccgtcc atcaccggat gattgttgca ataccccgtg
     1021 gcatcagaga ctgaacagcc gtttttaacg ccacgtattg ccctgatgaa ccaggccggg
     1081 caacccgaaa acatctttcc acccggcatt gtccgccagc acttatcacc gaccaggtct
     1141 gttgcagtaa atccaccagt tgcgcacatt cttcatcagc catgacgccg actcgcggat
     1201 ggcaaatact cgtctggctg agcagcaaca gacatcgtat ctgtgctcct tcatgttgtt
     1261 cccgcaacca gcgtaatacc accggcgctg atgggctgac cgtgtgatta tgctccgtaa
     1321 tacgttcgtg tacccgtcgg tgaaaggaac tacaaaatgt ctgaatctgt tgaccataga
     1381 ttccttctgc cgaatagagc gtgaagtgaa atgccaacag atttggccag tggtccaccg
     1441 cctgctgaac aaaacgccgg atttcccccg gctctgaaat taaggcttcg gttatttgca
     1501 cgaaatgttc cttttcaacc cctgttgaat tttgtcaagc cggtgcagac acagtaaagc
     1561 gcgcatcaac acctgaaagg tacgatacaa acacaagtta caaacaataa gaacggaagg
     1621 acaattaatc agtgaggaat aataactaaa ttaaccaaaa gaatgttact gacaatacta
     1681 ccaatactta aatagcaata tattaattaa tgtaagaaca gatgcccggt aagacatata
     1741 cacaatacat ccacaaacac actcttttca ttaaatcaat cacctgatta aaccatgaaa
     1801 gtgtga
//
LOCUS       DAZKFU020000464         1805 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZKFU020000464 DAZKFU020000000
VERSION     DAZKFU020000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.466"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..325
                     /gene="bssS"
                     /locus_tag="ACHOQQ_RS24625"
                     /old_locus_tag="pgaptmp_004925"
     CDS             71..325
                     /gene="bssS"
                     /locus_tag="ACHOQQ_RS24625"
                     /old_locus_tag="pgaptmp_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGU4473053.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            440..1558
                     /gene="solA"
                     /locus_tag="ACHOQQ_RS24630"
                     /old_locus_tag="pgaptmp_004926"
     CDS             440..1558
                     /gene="solA"
                     /locus_tag="ACHOQQ_RS24630"
                     /old_locus_tag="pgaptmp_004926"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGU4473054.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1606..1719
                     /locus_tag="ACHOQQ_RS24635"
                     /old_locus_tag="pgaptmp_004927"
     CDS             1606..1719
                     /locus_tag="ACHOQQ_RS24635"
                     /old_locus_tag="pgaptmp_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HGU4473055.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          466 a          437 c          434 g          468 t
ORIGIN      
        1 aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta
       61 gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga
      121 catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa
      181 taagtccgag caggaaggga ctgaagttgg tcagacgctc tggttaacca ctgatgttgc
      241 cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttccc
      301 ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc
      361 ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca
      421 tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg
      481 cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgcttatga ccgacgccca
      541 tatgccacca catcaacacg gcagccacca cggcgatacg cgattaattc gccatgctta
      601 tggtgaaggc gaaaagtatg tcccactggt cctccgcgcg caaacactgt gggatgagct
      661 ctcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggccc
      721 ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt
      781 tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa
      841 ttacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg cgattaaaac
      901 ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc cggtcaccgc
      961 aattcgtcat gacgatgatg gcgtaactat tgaaacggct gacggtgagt atcaggcgaa
     1021 aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc tgcctgtcca
     1081 gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg tgaagaataa
     1141 attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga
     1201 aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga
     1261 acgtgttccg tttgcggaag tggccagtga tgggtcagaa gccttcccgt tcttgcgcaa
     1321 tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc
     1381 tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc tcattaccgg
     1441 cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg ccgattttgc
     1501 gcaagacaaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct tccaataatc
     1561 atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggctgttg
     1621 cactatctca tcaataatat tcgcgagcat ctgatgcttt atctttttct ctggggattg
     1681 ttggcaatta tggatctgat ctatgtgttt tatttttagt tttccgcact gacataaact
     1741 gacaaatatt taatctccct ttcatttagc gtgaaaaatt gaatgagatt attcctgctt
     1801 ctaca
//
LOCUS       DAZKFU020000465         1792 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZKFU020000465 DAZKFU020000000
VERSION     DAZKFU020000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.467"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..417
                     /locus_tag="ACHOQQ_RS24640"
                     /old_locus_tag="pgaptmp_004928"
     CDS             <1..417
                     /locus_tag="ACHOQQ_RS24640"
                     /old_locus_tag="pgaptmp_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4473056.1"
                     /translation="TAYRDGAGIWTICRGATRVDGKPVIPGMKLSKEKCAQINAIERD
                     KALAWVAKNIRVPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRW
                     WIKDGGRDCRIRSNNCYGQVIRRDQESALTCWGIDR"
     gene            638..751
                     /locus_tag="ACHOQQ_RS24645"
                     /old_locus_tag="pgaptmp_004929"
     CDS             638..751
                     /locus_tag="ACHOQQ_RS24645"
                     /old_locus_tag="pgaptmp_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473057.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIIAIAVLIWAIRWW"
     gene            753..1247
                     /locus_tag="ACHOQQ_RS24650"
                     /old_locus_tag="pgaptmp_004930"
     CDS             753..1247
                     /locus_tag="ACHOQQ_RS24650"
                     /old_locus_tag="pgaptmp_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4473058.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGP
                     VREATGTARVDNATGPQLADTVTRDYFTLRERLMTMQKQLEGAQEYIRTQCIKLSFYY
                     PEDI"
     gene            1244..1468
                     /locus_tag="ACHOQQ_RS24655"
                     /old_locus_tag="pgaptmp_004931"
     CDS             1244..1468
                     /locus_tag="ACHOQQ_RS24655"
                     /old_locus_tag="pgaptmp_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473059.1"
                     /translation="MKKLLVTVKPFQGTIPFRVLQPGRVLVEGTFSGKCTQSYSRVFR
                     VDASNEELTVECAVNAAKCRMVSAALQPVC"
BASE COUNT          441 a          397 c          517 g          437 t
ORIGIN      
        1 acagcatacc gtgatggtgc gggtatctgg accatctgcc gtggtgccac ccgggtggat
       61 ggtaagcctg tgattcctgg catgaagctg tcgaaggaaa aatgcgcaca gattaatgcc
      121 attgaacgtg ataaggcgct ggcatgggtg gcgaaaaaca tcagagtgcc actgaccgaa
      181 cctcagaaag cgggtattgc gtcattctgt ccttacaaca ttggccccgg taagtgtttc
      241 ccgtcgacgt tttataaacg aattaatgca ggcgatcgca ggggagcatg tgaggcgatt
      301 cgctggtgga ttaaggacgg tggcagggac tgccgtattc gctcaaataa ctgctacggg
      361 caggttatac gtcgtgacca ggaaagcgcg ctgacgtgct ggggcatcga cagataagca
      421 gaatattttg ctgaaaaatg acgttgacca acgcgaacgg ataacacgaa atcctgcgaa
      481 ctggcaaaat ctaagtgaat aaaagtaaaa accccgtttg ttggcagcaa gcggggtttt
      541 gtgtttctga ccttggataa ggcaagggag aaattatggt gagggaggtg ctttccctgt
      601 gaggaagtat aaaagattct ttctgaggtt gtccattatg aaaggcattg aagtggagac
      661 gccagccagt ctggatttaa caagagcggc agcttttgcc attcgtatta tagccattgc
      721 tgttctgatc tgggcaatcc gttggtggtg aaatgaaccg cgttctgtgt gtggtcatca
      781 ttgctctgct ggtggcctgt ggtgcgctta gtctggggct gaatcattac cgcgataacg
      841 ccatcactta caaagcgcag cgcgataaaa aagccagaga gctggagctg gcaaacgcaa
      901 ccattactga tatgcaggtg cgccagcgtg atgttgctgc gctcgatgca aaatactcga
      961 gggaattagc cgatgcgaga gctgaaaatg aaactcttcg cgctgacgtt gccgctggtc
     1021 gtaagcgcct gcgtatcaac gccacctgtc caggtcccgt gcgtgaagcc accggcaccg
     1081 cccgcgtgga taatgcaacc ggcccccaac tggcagacac cgttacacgg gattatttca
     1141 ccctcagaga gcggctgatg acgatgcaga agcaactgga aggggcgcag gaatatatcc
     1201 gtactcagtg cattaagttg tctttttatt atccggaaga tatatgaaga aattactggt
     1261 aaccgtaaag ccttttcagg gaacaattcc gttccgtgtt ttgcagcctg gtcgtgttct
     1321 tgttgaaggt acgttcagtg gtaaatgtac gcaatcatac tcccgggtct ttcgggtgga
     1381 tgcctcgaat gaagagctaa ccgtggagtg tgctgtgaat gccgctaaat gccgcatggt
     1441 atcggctgca ttacagccag tgtgttgagc gaccttatta tccatggcgc ggtattgtcg
     1501 ccgtattccc gtattaacag agaccgcagc ccgacaggga gactcctctg cgcgagtgtg
     1561 cggggataat caaaaacgat acacaccggg gtttaccgcg ttaacggagc gcggcgttgt
     1621 cccctcatgg tcgctggtcc ggtgcgatgg tggaagaaac cggacgatgt gttacctcgc
     1681 atgacctgtt atgtcatgtg tctgatttgt gatttaagtc ggataattgt cgttgccatt
     1741 aagcagagga ttgatggccg acagggtggc attgttagaa taagacttat tc
//
LOCUS       DAZKFU020000466         1792 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZKFU020000466 DAZKFU020000000
VERSION     DAZKFU020000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.468"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1035)
                     /locus_tag="ACHOQQ_RS24660"
                     /old_locus_tag="pgaptmp_004932"
     CDS             complement(<1..1035)
                     /locus_tag="ACHOQQ_RS24660"
                     /old_locus_tag="pgaptmp_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4473060.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLAL"
     gene            1187..>1792
                     /locus_tag="ACHOQQ_RS24665"
                     /old_locus_tag="pgaptmp_004933"
     CDS             1187..>1792
                     /locus_tag="ACHOQQ_RS24665"
                     /old_locus_tag="pgaptmp_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4473061.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIM"
BASE COUNT          552 a          439 c          406 g          395 t
ORIGIN      
        1 cagcgccagg cctgcatcca tcgcattgaa gaagagggag tttgcgacac cgattttatg
       61 cggttccact gaactgataa tctgcgtctg aaaaactggc gtcacggaac catacccgat
      121 accaatcaac gcgccagaaa cgaccatcat tacgctgccc tgggtgtagc cgagaaccac
      181 cagcccaacc gtaaagaaca gcaggcaggg gtaaaccaca tatttcgggc cttttttgtc
      241 gcaaacattg ccggtgaagg tacggcagat catcaggaag atggcgtagc agagcaggaa
      301 attactggcc gccgacatga gattaagttc gcgggcgtaa agcgccagaa aggcagaaac
      361 cccggcatac gaaaaggtca taaagaatgt aaccatggca aacggcaatg ccgcgcgatc
      421 gaacatggcg gcgaagccca tctttggctt tgaaccatcg gcatggctga taaccggcgg
      481 cacggtcata atcagcgaca ggataatccc caccgccgca acgccagtac ataaccagaa
      541 agccatatta aaggcctccc agcgcgccat attcaggcca atccacggcc cgaccaccat
      601 tgccagcccc attgccagag agaaaaaact gatgccctcc ccacgtcggg aggccgggat
      661 cagccgcgcg gaaatcgttc cctttaccgt tgtgataacc ccgaaggtaa taccatgaag
      721 caccctaata aaaagcagtg attctattga gtggcaaaaa ggatacagcg cagtgaccac
      781 caaaaaggcc agagaagaga gcaccagaat agttttattc gaatatttac ccacccattg
      841 tccggcaaag ggtcgaataa caatcgccgc aattaaaaat aatgtcacca acaaacctgc
      901 tttatcagga gaggcatgca agcggtcaga gatataaatt ggcaacaatg ttagtaaaac
      961 ataaaagacg aagaaaataa taaagctgat gatggttatt gcccaaaaat ccttcgtcca
     1021 taacttctct ttcataccct accgcctgtc aggatgacgc acgcagacaa cactcccgtt
     1081 actgcggttg cgtcaaattg tgatgtactt tgcagaacag aagtataaat ggcggcatgc
     1141 tattagagaa attaatcgtt ttaatattat tgattagcaa actctaatga ctcttaccca
     1201 aataaattct ttactcgccg tgctggatta cggtggcttt actgaagcca gcaagcgatt
     1261 gttcatgacg cagtcggcgg taagtcaggc aattgctgcc ctcgaacaag aacttggtgt
     1321 gaatatactg ttacgcgatc gccgtaaagc tatacaactg accacagcag gtcaccgcat
     1381 tgtgcaacat ctgcgcgcca ttaatcggga agttaacgca gtaaaagaga tagccgagca
     1441 ggaaaagcag aaccctcaaa gaacgctgag acttggttgt ttccccagcg tctgcgcctg
     1501 tatcttaccg gttgtgattc gctactttga aattcatcat ccgaatataa aaattattcc
     1561 ttttgaggaa aatagcacag cgattattga ttcgcttcag aatgaatcca ttgatgccgg
     1621 ttttgttcat tttccggtga gcggaatgta ctccgtgccc atttatcagg ataaattcac
     1681 cgtagtgtta ccgcccaacc atgctctggc aaaaaacagc actattacag tggaagaatt
     1741 aatgggtgaa ccattaatca ttagtaaagg acgctatgaa ttaagcatta tg
//
LOCUS       DAZKFU020000467         1772 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZKFU020000467 DAZKFU020000000
VERSION     DAZKFU020000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.469"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(35..232)
                     /locus_tag="ACHOQQ_RS24670"
                     /old_locus_tag="pgaptmp_004934"
     CDS             complement(35..232)
                     /locus_tag="ACHOQQ_RS24670"
                     /old_locus_tag="pgaptmp_004934"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HGU4473062.1"
                     /translation="MIGKAIIKAQYNKQVPLTLVAMSVLTAMSIACQNQVDVCSPGNL
                     RGAVNIYTMVLEKGKRPGERY"
     gene            complement(459..755)
                     /locus_tag="ACHOQQ_RS24675"
                     /old_locus_tag="pgaptmp_004935"
     CDS             complement(459..755)
                     /locus_tag="ACHOQQ_RS24675"
                     /old_locus_tag="pgaptmp_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4473063.1"
                     /translation="MPDQSGYCWTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            complement(978..1671)
                     /locus_tag="ACHOQQ_RS24680"
                     /old_locus_tag="pgaptmp_004936"
                     /pseudo
     CDS             complement(978..1671)
                     /locus_tag="ACHOQQ_RS24680"
                     /old_locus_tag="pgaptmp_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          480 a          381 c          370 g          541 t
ORIGIN      
        1 tttctcattc ttaatacctc ctgaaattat ttcactaata cctttccccc ggtcttttcc
       61 ctttctccag aaccatagtg taaatattaa cggccccacg taaatttccg gggctgcata
      121 cgtcaacctg attctgacag gcaattgaca tcgctgttaa tactgacatt gcaaccagag
      181 tcagaggtac ttgtttattg tattgagctt taattattgc cttccctatc attgatggtc
      241 acgcatatac tgaatacggc atatccggtt tgatgtaaga cataaatgct tcctttttcg
      301 taatacgttt cggttatagc agcaattacc gctacaatag cgacaatagc tgctctttca
      361 acaatgaatg ctggaaattc tgtttatcaa ttaaacttct ggctaaattg ttcttatgct
      421 tctttttcct cctgaggtga tcacgtgatg acaaaaactt atctgctcat ggtcatatct
      481 gaagaaagcg tatgtcgcac ttcgtcatta acacctttga ctgctgatct ggcaggtaca
      541 gtatgtaaag taagccattc gtcaatagct gatgaacgcc agcccacaga accactacca
      601 agacgtaccg gcctggggaa ggttgcatcg tagtatttcg ataacggatt cattttttcg
      661 taaattgtcg aacgagatat acctaacaat tggctcagtt caggcatccg taagatgcgt
      721 gaaggagtcc agcagtatcc ggattgatct ggcattttat cccccatagt gtggtgaact
      781 gtcgttacag tttgtattcc ccgggtaaat ttgtctgcgt aaaaaaatgg gctgagcaaa
      841 aagacaataa ataattaaaa atcaatacga tagaaaacaa cagcattatt gttattttac
      901 tttctcctta tcgctgtcga ttctcattat cattcattgg taaaaaacag caaaagaggc
      961 cctggtaatg actccagtca gtagtacagt gttttcgaca aatgaaccaa tggcttttcg
     1021 tgaatttcca gggagcgtga aaagcaaatc gttttccggg ctggtcgttt gatgtgagta
     1081 cgaaatgtca gattgttgcg ctcaatgcgc cgggtaaata tcttgccgac aagatgctta
     1141 tcctgtggca tttctctggt ataactgctc cggttatctc tggttatcat gcctgtggag
     1201 aatggcttca ggagttccgg caactcacga catgtttcat cagtgcgagg accaaaagtg
     1261 taagccagca cacctccaga tttggtgtta tacgcatacc agtgccactg ctgacgagct
     1321 ttattttcga caaaactcca ttgttcatca agttcgcaga taagcgtcac atcggcactg
     1381 gcgactggag atgtggttac tttgcgtggt gagcgtttta taaagtgaga atgacggtgt
     1441 tgatatccac cttcagcgtt ctggagctat cgcgaacccc gactccgtta tgaaccattt
     1501 caacaatttg ctctttaatg cctggttttc gggtttcgta gatgtaatgc agttgaaata
     1561 cacacgtttg caggattagc actggaaacg ctcatgatct gaggtgctgt gatcataatg
     1621 gtacacttta taggaattac agcggggaga atgtactgta actgttgcca tatggtctcc
     1681 agataccaat agaataccac attaatctat cgtcagaagg catcaccggg gccttctgac
     1741 ataatctgtt aatacgtgta ctattaccct gt
//
LOCUS       DAZKFU020000468         1770 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZKFU020000468 DAZKFU020000000
VERSION     DAZKFU020000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.470"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1770
                     /locus_tag="ACHOQQ_RS24685"
                     /old_locus_tag="pgaptmp_004937"
     CDS             <1..>1770
                     /locus_tag="ACHOQQ_RS24685"
                     /old_locus_tag="pgaptmp_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU4473064.1"
                     /translation="AFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFIARGGS
                     LAGTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVEKSGSGTLTVSNT
                     TLIQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATW
                     NIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQ
                     NNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVIEAINGATTEEGAFVQGN
                     MLQAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGV
                     NGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKA
                     SSGNNDFRARGWGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFG
                     HGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSS
                     RGDMRVGTSTAGSGMTFSPSQNGT"
BASE COUNT          401 a          507 c          534 g          328 t
ORIGIN      
        1 ggcattccgt atcggaggcg gtcaggcgga tgccctgatg ctggaaaaag gcagttcatt
       61 cacgctgaac gccggtgata cggccacgga taccacggta aatggcggac tgttcatcgc
      121 cagagggggc tcgctggcgg gcaccaccac gctgaataac ggtgccacgc ttaccctttc
      181 cgggaaaacg gtgaataacg ataccctgac catccatgaa ggtgatgcac tcctgcaggg
      241 aggcgctctt accggtaacg gcagggtgga aaaatcagga agtggcacac tcactgtcag
      301 caacaccaca ctcatccaga aaaccgtcaa cctgaatgaa ggcacgctga cgctgaacga
      361 cagtaccgtc accacggatg tcatcgctca gcgcggcacg gccctgaagc tgaccggcag
      421 cacagtgctg aacggtgcca ttgaccccac gaatgtcacc ctgacatccg gtgccacctg
      481 gaatatcccc gataacgcca cggttcagtc ggtggtggat gacctcagcc atgccggaca
      541 gattcatttc acctccgccc gcacagggaa gttcgttccg acaaccctgc aggtgaaaaa
      601 cctgaacggg cagaatggca ccatcagcct gcgtgtacgc ccggatatgg cgcagaacaa
      661 tgctgacaga ctggtcattg acggtggcag ggcaaccgga aaaaccatcc tgaatctggt
      721 gaacgccggc aacagtggta cggggctggc gaccaccggt aagggtattc aggtgattga
      781 ggccattaac ggtgccacca cggaggaagg ggcttttgtc caggggaata tgctgcaggc
      841 cggggccttt aactacaccc tcaaccggga cagtgatgag agctggtatc tgcgcagtga
      901 agaacgttat cgtgctgaag tccccctgta cgcctccatg ctgacacagg caatggacta
      961 tgaccggatt ctggcaggct cccgcagcca tcagaccggt gtaaacggtg aaaataacag
     1021 cgtccgtctc agcattcagg gcggccatct cggtcacgat aacaacggcg gtattgcccg
     1081 tggggccacg ccggaaagca gcggcagcta tggcttcgtc cgtctggagg gtgacctgct
     1141 cagaacagag gtcgccggta tgtcactgac gacaggagtg tatggtgctg caggccattc
     1201 ttccgttgat gttaaggatg atgacggctc ccgcgccggc acggtccggg atgatgccgg
     1261 cagcctgggc ggatatatga acctgacaca cacctcctcc ggcctgtggg ctgacattgt
     1321 ggcacaggga acccgccaca gtatgaaagc gtcatcgggc aataacgact tccgcgcacg
     1381 gggctggggc tggctgggct cactggaaac cggtctgccc ttcagtatca ctgacaacct
     1441 gatgctggag ccacggctgc agtacacctg gcaggggctt tcgctggatg acggtaagga
     1501 caacgccggt tatgtgaagt tcgggcatgg cagtgcacag catgtgcgtg ccggtttccg
     1561 tctgggcagc cacaacgata tgacctttgg tgaaggcacc tcatcccgtg cccccctgcg
     1621 tgacagtgca aaacacagtg tgcgtgaact gccggtgaac tggtgggtac agccttccgt
     1681 tatccgcacc ttcagctccc ggggagatat gcgtgtgggg acttccactg caggcagcgg
     1741 gatgacgttc tctccctcac agaatggcac
//
LOCUS       DAZKFU020000469         1763 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZKFU020000469 DAZKFU020000000
VERSION     DAZKFU020000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.471"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1354
                     /gene="sctC"
                     /locus_tag="ACHOQQ_RS24690"
                     /old_locus_tag="pgaptmp_004938"
     CDS             <1..1354
                     /gene="sctC"
                     /locus_tag="ACHOQQ_RS24690"
                     /old_locus_tag="pgaptmp_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HGU4473065.1"
                     /translation="TNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNAA
                     KLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIKQ
                     STGVNVLSEISSRQQLKNVSRMPPFPRAEEDDDLQVEKIISTAGAPETDDIQIIAYPD
                     TNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKIG
                     SSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRTF
                     YTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQV
                     GRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNIV
                     RVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVGG
                     "
     gene            1359..>1763
                     /locus_tag="ACHOQQ_RS24695"
                     /old_locus_tag="pgaptmp_004939"
     CDS             1359..>1763
                     /locus_tag="ACHOQQ_RS24695"
                     /old_locus_tag="pgaptmp_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0046903 - secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrpJ domain-containing protein"
                     /protein_id="HGU4473066.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQ"
BASE COUNT          594 a          302 c          400 g          467 t
ORIGIN      
        1 gacgaatgag tttaataatt tcttaaaaaa atcgggtctg tataactctc gttatgaaat
       61 taaagggggc ggcaatggta ctttctatgt atcggggcca ccggtttatg ttgacctggt
      121 cgttaatgct gcgaaattaa tggagcaaaa cagcgatggc attgagatcg gtcgaaataa
      181 agtaggaata attcatctgg tcaacacatt cgttaatgac cggacttatg agttacgtgg
      241 cgaaaagata gttattcctg gcatggcaaa agtactatcg acgttgttaa ataataacat
      301 taagcaaagc acaggggtga acgtactttc tgaaatttca agtcgtcaac aactaaaaaa
      361 cgtatcacgt atgcctcctt ttcctagggc tgaagaagat gatgacctac aagtagagaa
      421 aataatatcg acagcaggag cgccagagac tgacgatata caaattattg catatcctga
      481 taccaacagt ttacttgtta aaggaacagt atctcaggtt gattttatcg agaagttagt
      541 agctacgctt gatattccaa agcgacacat tgaattatct ttatggataa tagatattga
      601 taagactgat ctagaacagc taggtgctga ctggtcgggc acgataaaaa ttggatccag
      661 cttaagtgca tcatttaata attcaggttc aattagtacc cttgatggga cacagtttat
      721 tgcaacaata caggcacttg cacaaaaaag aagagccgcc gttgttgctc gtcccgtcgt
      781 tttaacacag gagaatattc ctgctatatt tgataataac cgcactttct atactaaatt
      841 agtcggcgag cgtactgcgg aattggatga agtaacctat ggtacgatga ttagcgtgct
      901 accacgattt gctgcgcgaa atcaaattga attactcttg aatattgaag atggaaatga
      961 aatcaattcc gacaagacca atgttgatga tctgcctcag gttggtagaa cacttataag
     1021 tactattgca cgcgtgcccc aagggaaaag tcttttgatt gggggatata cacgcgatac
     1081 gaatacctat gaaagtcgca agatcccaat attaggcagc attccattta ttggtaaatt
     1141 atttggttat gaaggaacaa atgcgaataa catcgttcgt gtctttctta ttgagccaag
     1201 agagatcgat gagcgcatga tgaataatgc gaatgaggct gcggttgatg ccagagcgat
     1261 tacacagcaa atggcaaaaa ataaagaaat taacgatgaa ttactgcaga aatggataaa
     1321 aacttacctg aatcgtgaag tcgtcggagg atagtgaaat ggcgattcat gtagagcatg
     1381 tcggtgtatt agaaagagcc agggaagttt ctcgcttaga ggacattatc acagaagata
     1441 atgaagatat tgaagctgaa atgcctaaaa tgggggatga tcccgcaggt aaagaagctc
     1501 ggttcttaca agctactgac gagatgtcag cagcgttaac gcagtttatg aagaaaaaaa
     1561 tctacgaaga acaactggcc aattttctgg acggagaaga atatgtcctg gaagaccagc
     1621 caatagaaaa aaccgacaaa gttatggagg cgcttaaggc tgcaactacc catgactatg
     1681 aagtgtatag ctttgctaaa aaattattcc cagatgaaag cgatctggta gttgtgttac
     1741 gtgcaatttt acgtaaaaaa caa
//
LOCUS       DAZKFU020000470         1762 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZKFU020000470 DAZKFU020000000
VERSION     DAZKFU020000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.472"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..315
                     /locus_tag="ACHOQQ_RS24700"
                     /old_locus_tag="pgaptmp_004940"
     CDS             <1..315
                     /locus_tag="ACHOQQ_RS24700"
                     /old_locus_tag="pgaptmp_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HGU4473067.1"
                     /translation="RTQSRRLKLSTRRARAAQFRDGWVQGIRQVVMPFSVTEEESRLM
                     RLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNASLHHGVGGTARQMVPGLMIRE
                     AS"
     gene            312..926
                     /locus_tag="ACHOQQ_RS24705"
                     /old_locus_tag="pgaptmp_004941"
     CDS             312..926
                     /locus_tag="ACHOQQ_RS24705"
                     /old_locus_tag="pgaptmp_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HGU4473068.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKVDED"
     gene            930..1328
                     /locus_tag="ACHOQQ_RS24710"
                     /old_locus_tag="pgaptmp_004942"
     CDS             930..1328
                     /locus_tag="ACHOQQ_RS24710"
                     /old_locus_tag="pgaptmp_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473069.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VASCIIEDGCMCVDGVGEYVSHSLPGGTYQLYTASPVPVVPEGLVKAVRFYEQVKREN
                     PPVETGAWKDAVDWVLKEACQAVNIRIKGE"
     gene            1331..>1762
                     /locus_tag="ACHOQQ_RS24715"
                     /old_locus_tag="pgaptmp_004943"
     CDS             1331..>1762
                     /locus_tag="ACHOQQ_RS24715"
                     /old_locus_tag="pgaptmp_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HGU4473070.1"
                     /translation="MQISPVTLRVAKAFISRHHRHNKPPVGHKFSIGLRNDAGELTGV
                     ATAGRPVARHFDDGLTLEVNRTCTTGERNANSALYGAVWRAAKDMGYRRCITYTRADE
                     SGASLRAAGFVRVKELPSGKSWAESSVALKSTRDPVGSGGVP"
BASE COUNT          403 a          391 c          578 g          390 t
ORIGIN      
        1 cgtacacaga gccggagact gaaactgagc acccgtcgtg cccgggcggc tcagtttcgt
       61 gatggctggg ttcaggggat tcgtcaggtg gtgatgccat tcagtgtgac agaagaggaa
      121 agccggctga tgcgtctctg gatggaacag caggatatgg tgacggtgga ttcccgggag
      181 atgaaagcct gtcgcggcgg tgacctggcc cgctggcagg ggtatgaggc cgggaaaaat
      241 gcctcactgc atcatggtgt ggggggaact gcccgccaga tggtgccggg tctgatgata
      301 cgggaggcgt catgaataac agcttgtctc aggcaaccaa cggtctgctg atgcaactgg
      361 tgatggacct caagagcggc tacctgcgtc gctgcgaatc tctggggctg gcccgggagg
      421 aaatgcagat gctgcaggga ctgaccattg aggaaattca ttacctgtca aactctgaag
      481 tgtcggttat ccgtctggac attaatcatg aaaacctgct gcgtatgctg cagcaggccc
      541 ggacggagca gaaacgtctg cagcgtattg accgggcact ggcgctggga ggctccattg
      601 agctgatggc gttttacttt ggactttcca gtgtggatgt ggcggcgcgc cggcgcattg
      661 ccggtattga tgttcgcccg ggacgtggca tcacactgag tgatgaggaa agtgcagagc
      721 tgtggcgttt atggcagaag agcggcatca cagatgtgga aagtgcggac ggactggatg
      781 tcatgatgct ggcagctgag caaatgaata tcccgctgac ggcggtatgg catgcagtga
      841 ggggatggca tcagggaact gcggggttta atgatgtatc ggccagtcgc cggaaactga
      901 accgggaaaa agtggatgag gactaaccta tgaccactat taccaaagag cgactgcaat
      961 ggctggctaa catttctggc cgcgatgaca ttgacgatat agacggcggt gagattcgtg
     1021 agctggcgct tatcgcgctg gcatcgctgg aagcagagcc tgtggcgtca tgcattattg
     1081 aagatgggtg catgtgtgtt gacggggtcg gtgagtatgt aagtcactcg ctgcctggtg
     1141 gaacgtacca gctatacacc gcttcacctg tgccggtagt gcctgaagga ctggttaaag
     1201 cggtgcgctt ttatgaacag gtaaagcgtg agaatccgcc agttgaaact ggggcatgga
     1261 aagatgcggt tgactgggtg ctcaaagagg cttgccaggc tgtaaatatt cgcatcaaag
     1321 gagagtgagc gtgcagattt caccagtcac tcttcgtgtt gcgaaggcgt ttatatccag
     1381 acatcaccga cacaataaac ctccggtggg gcataaattc agtattggtc tgagaaatga
     1441 tgcgggtgaa ttgacaggtg tggcgacagc cggcagacct gttgcacgcc actttgacga
     1501 tgggctgaca ctggaggtca atcgtacctg tactactggt gaacgtaacg ctaacagcgc
     1561 actttatggc gctgtctggc gggcggcaaa agatatgggc tatcgccgtt gcattacata
     1621 caccagggct gatgaatccg gagcctctct tcgggcagct ggttttgttc gtgtgaaaga
     1681 acttccttca ggaaaaagct gggcggaatc aagtgtcgcc ctgaagagta cacgcgatcc
     1741 ggttggaagc ggtggtgttc ct
//
LOCUS       DAZKFU020000471         1743 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZKFU020000471 DAZKFU020000000
VERSION     DAZKFU020000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.473"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..1553
                     /locus_tag="ACHOQQ_RS24720"
                     /old_locus_tag="pgaptmp_004944"
     CDS             120..1553
                     /locus_tag="ACHOQQ_RS24720"
                     /old_locus_tag="pgaptmp_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU4473071.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     FSSACQNQIDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKLHKEIRAGKDCKATESELKRHLNKCPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGIIFDGRAMSKLGILNSLWDGGSLFIDRKSS
                     PGIILKDPRLTVSVMIQPDVYQKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEQRINELIDENLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIHHSSGEEGDISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNPALTVPVRLIPEELELFNWIKSYCIEKGIPCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT          527 a          323 c          416 g          477 t
ORIGIN      
        1 atcagatcta caaatgctct gtatttctca gagcatcagg tctgaatagc agctattttc
       61 gctattgtag cgggatttgc tgctatttta ctaacccatt actgaaaaga gaggctttta
      121 tgtgcttact tgcaccagaa aatccttatc cgatatatgc actaccaccg ctggtgagaa
      181 atgcaataat tgaaactcaa aaaaatacac aggcaccttt ggctatggtg gcgacatccg
      241 cattaacagc gttctcaagt gcctgtcaga atcagattga cgtgtgcaga cctggaaatt
      301 tacgtgggcc tgttaatctt tactctctga ttctggctga ttccggtgaa aggaaaacga
      361 ccgtggataa agtgtttatg aaagcatttt atctcaggga tgaagccctg gcggaagaat
      421 acgcgaaact ggttgagaat tacagtacag aaaaggaaat atgggagcaa aagcaaaaag
      481 cactggagtc aaaattgcat aaagaaattc gcgccggtaa agattgtaag gcaacagagt
      541 cagagcttaa aagacatctg aataaatgtc ctgttccgcc gcagatacgc cggacaattt
      601 tcaatgagac aacgatagag ggaatgttaa aatattattc cgacagcaat cgctcttttg
      661 ctcttgtatc cagtgaagga ggaataattt ttgatggccg ggcaatgagt aaacttggaa
      721 tacttaactc tttgtgggat ggtggttctc ttttcatcga caggaaatca tctcccggaa
      781 ttattttgaa ggatccaaga ctgacggtat cggtgatgat tcagcctgat gtttaccaga
      841 aaggtttttg tacgcgaaaa aaagaacttg tgaaaacgtc aggacatcat gcaaggtttt
      901 tgatgtgtca accaacatca acgcagggga caaggataat aaccggagat aattattcat
      961 cacagtatca ggatttgttt gaacagagga tcaatgagct tattgatgaa aatctggcga
     1021 tgagtggtga acgacgttgc cttcactttt ctcctcaggc tgccagaatc tggacggatt
     1081 attacaacga tgtggaatcg aagctggggg gattggggcc tttaagacat tgccgggaat
     1141 atgctgctaa aaatgcagag tatatggcaa gactggcagg acttattcac cattcgagcg
     1201 gtgaagaggg ggacatttcc ccttacactg cagaaatggc gagagaatta gcaatatggt
     1261 acggtaatga gtatgtgcgg ttgtctaatc cgttaacttt tgacaaccct gccctgaccg
     1321 tacctgtgcg actcattcca gaggagcttg aacttttcaa ctggataaaa agctattgca
     1381 ttgagaaggg gatcccctgt atgaaaaaaa atgatatttt acagcgtggt ccgaaccgtt
     1441 tccggaaaaa ggataaaatc aactggttac tggatttact gtatgaacaa aacagagttg
     1501 taccggttat tgagggaaaa acgttgtgtg tcgcacctaa ctttgacctc tgaacgactt
     1561 aatcgttgag taacatatag tctcctctgc tacttgcgga ggagactaat gcctttacca
     1621 cgtcagcgtt gttgtattag aaaactctgg tacaggtcag tcagtaaata taactggtgt
     1681 aaccactatt tactattgga tagagtgttg ctcaggatat tgtcggcttt agtctgaggt
     1741 gag
//
LOCUS       DAZKFU020000472         1743 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZKFU020000472 DAZKFU020000000
VERSION     DAZKFU020000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.474"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            333..851
                     /locus_tag="ACHOQQ_RS24725"
                     /old_locus_tag="pgaptmp_004945"
     CDS             333..851
                     /locus_tag="ACHOQQ_RS24725"
                     /old_locus_tag="pgaptmp_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU4473072.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1061..>1743
                     /gene="tssI"
                     /locus_tag="ACHOQQ_RS24730"
                     /old_locus_tag="pgaptmp_004946"
     CDS             1061..>1743
                     /gene="tssI"
                     /locus_tag="ACHOQQ_RS24730"
                     /old_locus_tag="pgaptmp_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HGU4473073.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVT"
BASE COUNT          436 a          435 c          441 g          431 t
ORIGIN      
        1 taaggaacat ctcaaaaatt atcaaaatga taattcagaa aggataaata caatatttat
       61 tcatgattca gctttgacca ttttatggag gcaatacgga ctttgaacag ttgggtatgg
      121 ttatccagat atatattatt aagttattgc tgaataagag atttaatgat tttaaaaata
      181 gtttttacga caaccgtaaa ttctagctcg gtttgtatgt aaaaaatact gaaaaatgat
      241 gcggttccgt gaagtgagct gcatctgcct gtctgggatt aacggcaggt cattgtaagt
      301 agtaattaac cacgttaaca aggagtttac aaatgccaac accgtgttat atctccatca
      361 ctggtcagac ccagggaaat attactgcag gtgcatttac cgctgattct gtcggcaaca
      421 tctatgtgca ggggcacgaa gacgagatgc tggtgcagga attcctgcat aacgttactg
      481 tcccgaccga tcctcagtcc ggtcagcctg cgggtcagcg tgctcacaag cctttcattt
      541 ttaccgtggc gctgaacaaa gcggttccgc tgatgtacaa cgcactggcc tccggcgaaa
      601 tgctgccaac cactgaactt cactggtggc gtacctctgt ggaaggcaaa caggagcatt
      661 atttcactac ccgcctgact gattcgacga ttgtcgatat gaaactgcat atgccgcatt
      721 gccaggaccc ggcgaaacgc gagttcaccc agttgctcga agtttcactg gcctaccgca
      781 aaattgaatg ggaacacgtc aaatctggca cctccggtgc cgacgactgg cgcgcaccgc
      841 tggaagcata agttaagcca acagcatccg gctatcaggc cggatgctgt ttttgtatta
      901 cgccgtagaa agaaaggttg tcagtaaggg gttacgttca cgggagatgg cggctgccgg
      961 aaacttcggc ggtagcgtgc ctgaacgcaa gcgtaacact gtactgtcac agccgcttta
     1021 acagcgaact gtctgttttt ccttaacccc ggaggttgct atgtcaaccg gattacgttt
     1081 cacgctggaa gtggacggcc tgccaccgga tgcttttgcg gtggtctcct ttcatctgaa
     1141 ccagtcactc tcttcgcttt tttccctcgc tctctctctg gtcagccagc agtttctctc
     1201 ccttgaattc cagcagatcc tcgacaaaat ggcctacctg acgatatggc agggcgatga
     1261 cgtacagcgc cgggtgaaag gtgtggtgac ctggtttgaa ctgggggaga acgacaaaaa
     1321 ccagaagctg tacagcatga aggtatgccc gccgctgtgg cgcacagggc tgcgccagaa
     1381 cttccgtatc ttccagaatg aggacatcga aagcatcctc ggcacgatat tgcaggaaaa
     1441 cggggtgacc gagtggagcc cgctgttcag cgagccacat ccttcccgtg agttttgtgt
     1501 ccagtacggt gagactgatt acgatttcct gtgccggatg gcggcggagg aaggcatctt
     1561 cttttatgag gagcacgcgc aaaaaagtac cgaccagagc ctggtcctgt gcgataccgt
     1621 gcgttatctg ccggagtcct ttgagatccc ctggaatccg aacacccgta ccgaggtgag
     1681 caccctctgc atcagccagt ttcgctacag cgcacaaatc cgcccttctt ccgtggtgac
     1741 caa
//
LOCUS       DAZKFU020000473         1740 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZKFU020000473 DAZKFU020000000
VERSION     DAZKFU020000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.475"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(144..285)
                     /locus_tag="ACHOQQ_RS24735"
                     /old_locus_tag="pgaptmp_004947"
                     /pseudo
     CDS             complement(144..285)
                     /locus_tag="ACHOQQ_RS24735"
                     /old_locus_tag="pgaptmp_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            820..1008
                     /locus_tag="ACHOQQ_RS24740"
                     /old_locus_tag="pgaptmp_004948"
     CDS             820..1008
                     /locus_tag="ACHOQQ_RS24740"
                     /old_locus_tag="pgaptmp_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HGU4473074.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            complement(1011..1200)
                     /gene="tnpB"
                     /locus_tag="ACHOQQ_RS24745"
                     /old_locus_tag="pgaptmp_004949"
                     /pseudo
     CDS             complement(1011..1200)
                     /gene="tnpB"
                     /locus_tag="ACHOQQ_RS24745"
                     /old_locus_tag="pgaptmp_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1187..1395)
                     /locus_tag="ACHOQQ_RS24750"
                     /old_locus_tag="pgaptmp_004950"
                     /pseudo
     CDS             complement(1187..1395)
                     /locus_tag="ACHOQQ_RS24750"
                     /old_locus_tag="pgaptmp_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          477 a          376 c          431 g          456 t
ORIGIN      
        1 gagattattc ttacggggcc gggcgaatgc tatctgaatc gtacggttca tagaaataaa
       61 tatttaccga catagccgga gggttttcag atgcaatccc tgactcactg cgaaatcgca
      121 tcgtatccga tcgcggactc gccgattctc agaacaggca gtccctgaga atcgcagaac
      181 gctagtgcac gatcgttgaa cacatcagct acggtgatcg atgttttact cgtattatag
      241 tttgcagtgt gccactttcg gggatgtagc tacgaacgtc tgctggcttc ccgatatgtc
      301 agtaaaaaat acccgccgta ttacagcaac ggcaggtaat ggcacatcca gaagagaagt
      361 attttattat atcctcattt ttatcatgtg gtgtcaggag aattcaattc ggaataagtt
      421 tgcattcagt ttgcattcag aaatgaataa ttaacatacc attttacatt cacaccgcac
      481 ctgagcctgt acatcatcat gtctccggca ccaggagaaa ataaatatat tcaggatatt
      541 tcgttttgtt caggataatt aatgtcactc tgtatggatg aaaaaactga cagcagaagt
      601 ctccactggc gttgacaatg tggcagaagg agggtgtcaa gaactctgaa aaataacgag
      661 agagacatgt tactctgata tctttgttgt attctgaggt gccatcggtg cccgtacaga
      721 atcatcgcat gagccattgc tgagacgctc actttttttc gcatgcttta ccggagtgat
      781 ggctcagccg gtaattttgt tgtcaggatg agaggcgata tggggaaacg atataaaata
      841 actgcgacaa taatcaaacc cggtaatatg ccggttcagt ggttgcgtta ccaggattca
      901 gaaatgacat tcgccgaatg tgaaaaactg tttgcacctg cgaaagagcc cggacggtat
      961 attcgtgtca gggtggaaga cttccggtgt gaagtagtga aggcctgacg cagccatacg
     1021 ccatgtttat cccggcgtag aacttttatg cgggaacgag ccttgttggt aaagacaaag
     1081 gcagcattgt cgtgccatgc agcgttaagt tctttccgga cgtactgtgt cagttttgtc
     1141 gatgccccgg cgcatattga cgggttttac gaccaggaat acgttatcag tcctcagtat
     1201 ggcaaaaagc ccggaaaaca tcggttaact gagaaggctg gcagcacatc cggataccac
     1261 cgggaaggaa ggggataagg ctcatccgac aatgatctgt tatcggccac tccatgggca
     1321 acagatactg aggcgaggaa tgattgcagg cgctctggtc cgtttttcgg cttttgcggc
     1381 atcctgtggt cgctgatgcg ccatgaagca atatgatgct tttctggtca agcgctcatc
     1441 gcattccagg gattgtttat tctttctcca ccgaaggtgg ttatacgaat accggaatgc
     1501 taatgaacga aatatttctg tgaagatggt agaacagaga tgtgacgcca tcgctgtcat
     1561 acgggaatgg gtatatatca ggatttttat aaccgtcagc gccctccctc acggcagggg
     1621 aataccactc cggtagcatt caggaaaagt atcatcggat gaatgcttaa aaaaagaaca
     1681 aatgactgta tccgctattg ccagtacacc tcagacttcg atgccagtcc gtgaacgaac
//
LOCUS       DAZKFU020000474         1728 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZKFU020000474 DAZKFU020000000
VERSION     DAZKFU020000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.476"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..589)
                     /locus_tag="ACHOQQ_RS24755"
                     /old_locus_tag="pgaptmp_004951"
     CDS             complement(113..589)
                     /locus_tag="ACHOQQ_RS24755"
                     /old_locus_tag="pgaptmp_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU4473075.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(605..1090)
                     /locus_tag="ACHOQQ_RS24760"
                     /old_locus_tag="pgaptmp_004952"
     CDS             complement(605..1090)
                     /locus_tag="ACHOQQ_RS24760"
                     /old_locus_tag="pgaptmp_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4473076.1"
                     /translation="MTTFSHSSTTPSVSVTTASGSDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(1181..>1728)
                     /locus_tag="ACHOQQ_RS24765"
                     /old_locus_tag="pgaptmp_004953"
     CDS             complement(1181..>1728)
                     /locus_tag="ACHOQQ_RS24765"
                     /old_locus_tag="pgaptmp_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4473077.1"
                     /translation="ITGQHVPEIILLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGE
                     VRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGD
                     EHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSID
                     TDIKLNRALWVMAETLLESMR"
BASE COUNT          390 a          453 c          494 g          391 t
ORIGIN      
        1 tgttggtgaa tacgcatggc ttcaccacgg gtgatgattt tcatgatgtg atacctctga
       61 agacagaaga taaaagtgaa aacaggtatg atgtggttgt gacggtgacg ggttaaagca
      121 gaccgtgttc ggcaaaggag aaaacctggc tgccaccgac tatcagatgg tccggcaccc
      181 ggatatccac caggcccagt gcctgtacca gacgttccgt aataagccgg tctgccttac
      241 tgggtgtgac ttcaccggac gggtgattgt gtgccagtac cacggcagcg gcattgtggt
      301 acagggcgcg tttaatcact tcccggggat ggacttccgt gcggttgatg gtgccggtga
      361 agagggtttc accggcaatc aactgattct ggttgttcag atacagcacc cggaattctt
      421 cacgctccag tcccgccatg ttcagaatca gccattcccg tgccgctcgg gtggaggtga
      481 aggccacgcc gggttcatga agatggcggt ccagggtttt cagggcccgc agaatgagac
      541 tgcgctcgcc gggcgtcatc tctccgggca gaaaggaaag ttgttgcatt gtgcttctct
      601 ccattcagtc gatgatgcgc ataatggcgc tgcattccgg atgctgcagg gcgtaatccc
      661 gcaaccggta ataatggacc gtcatggcat aacactccgt acgacaggca tgatgactgt
      721 acgtcatcag acaggcagca atgccggcgg cttccgggct catttcagcg cggttaccgt
      781 tcatggcatt gaacagtacc cagttttcgc catcatcgtc atccggttcg ggggccataa
      841 atgcgccacc gttgttcagt gtgtacagat tccagatacc accgcagtag tcttcgcaaa
      901 gacggtccat ccagccgaag atacggggct ccagggtcac ccactgtgga atgaggccaa
      961 aatgctgcgg ccagaagctg atgcgctgtt catcagggac tatggtggca accagctgag
     1021 gctggtcact ccctgatgca gttgttacgg aaacagaagg tgtggtggag ctatgcgaaa
     1081 aggttgtcat gaaatgattc cttataaaaa gcaaatgaat gaagaagact cgggggcagg
     1141 gacagacgtg agtcaggacg gcgctttcag gtaaacggta tcagcgcatg ctctccagca
     1201 gcgtttccgc catcacccac aatgcgcgat tgagcttgat gtcggtgtcg atactgtgaa
     1261 tggcacgggt atggatacgt tttccttttg cactacggcc ggaaatcccg cctttcagca
     1321 tattctcctg gatggtctga tatgcgctcc acaggtcctt accgtaatcc tcccggcgtc
     1381 gtggtgtcag aatgtcggca gtggtgacgg gttgatgttc atcaccataa cggtaagtca
     1441 gcgccgcctg tgccagtgcc tgacgtgccg gtggcggcag aaccagagac tgcatggcat
     1501 cacgcttttc ctcaatccgg tcaaaaacgc ccactacctc gtaagcccct tcaatgactc
     1561 tgtccaccac atttccccgg tggggcacac ggacttcccc cagagactga ccgcagacgc
     1621 agccgttctg gcagacgaat ctgaagtaac ccggcagcat ctggtagctg gaggtaccgt
     1681 catgggagtt gagcagaata atttcaggca catgctgacc ggttatct
//
LOCUS       DAZKFU020000475         1708 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZKFU020000475 DAZKFU020000000
VERSION     DAZKFU020000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.477"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..218
                     /locus_tag="ACHOQQ_RS24770"
                     /old_locus_tag="pgaptmp_004954"
     CDS             111..218
                     /locus_tag="ACHOQQ_RS24770"
                     /old_locus_tag="pgaptmp_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473078.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            424..1707
                     /gene="gltA"
                     /locus_tag="ACHOQQ_RS24775"
                     /old_locus_tag="pgaptmp_004955"
     CDS             424..1707
                     /gene="gltA"
                     /locus_tag="ACHOQQ_RS24775"
                     /old_locus_tag="pgaptmp_004955"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGU4473079.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
BASE COUNT          449 a          418 c          392 g          449 t
ORIGIN      
        1 atcctgaaca gtgatccagg tcacgataac aacatttatt taatttttaa tcatctaatt
       61 tgacaatcat tcaacaaagt tgttacaaac attaccagga aaagcatata atgcgtaaaa
      121 gttatgaagt cggtatttca cctaagatta acttatgtaa cagtgtggaa gtattgacca
      181 attcattcgg gacagttatt agtggtagac aagtttaata attcggattg ctaagtactt
      241 gattcgccat ttattcgtca tcaatggatc ctttacctgc aagcgcccag agctctgtac
      301 ccaggttttc ccctctttca cagagcggcg agccaaataa caaacgggta aagccaggtt
      361 gatgtgcgaa ggcaaattta agttccggca gtcttacgca ataaggcgct aaggagacct
      421 taaatggctg atacaaaagc aaaactcacc ctcaacgggg acacagctgt tgaactggat
      481 gtgctgaaag gcacgctggg tcaagatgtt attgatatcc gtactctcgg ttcaaaaggt
      541 gtgttcacct ttgaccctgg cttcacttca accgcatcct gcgaatctaa aattactttt
      601 attgatggtg atgaaggtat tttgctgcac cgcggtttcc cgatcgatca gctggcgacc
      661 gattctaact acctggaagt ttgttacatc ctgctgaatg gtgaaaaacc gactcaggaa
      721 cagtatgacg aatttaaaac tacggtgacc cgtcatacca tgatccacga gcagattacc
      781 cgtctgttcc acgctttccg tcgcgactca catccaatgg cagtcatgtg tggtattacc
      841 ggcgcgctgg cggcgttcta tcacgactcg ctggatgtta acaatcctcg tcatcgtgaa
      901 attgccgcgt tccgcctgct gtcgaaaatg ccgaccatgg cagcgatgtg ttacaagtat
      961 tccattggtc agccatttgt ttatccgcgc aacgatctct cctatgccgg taacttcctg
     1021 aatatgatgt tctccacgcc gtgcgaaccg tatgaagtta atccgattct ggaacgtgct
     1081 atggaccgta ttctgatcct gcacgctgac catgaacaga acgcctctac ctccaccgtg
     1141 cgtaccgctg gctcttcggg tgcgaacccg tttgcctgta tcgcagcagg tattgcttca
     1201 ctgtggggac ctgcgcacgg tggtgctaac gaagcggcgc tgaaaatgct ggaagaaatt
     1261 agctccgtta aacacattcc ggaatttgtt cgtcgtgcga aagataaaaa tgattctttc
     1321 cgcctgatgg gcttcggtca ccgcgtgtac aaaaattacg acccgcgcgc caccgtaatg
     1381 cgtgaaacct gccatgaagt tctgaaagag ctgggcacca aagatgacct gctggaagtg
     1441 gctatggagc tggaaaacat cgcgctgaac gacccgtact ttatcgagaa gaaactgtac
     1501 ccgaacgtcg atttctactc tggtatcatc ctgaaagcga tgggtattcc gtcttccatg
     1561 ttcaccgtca tcttcgctat ggcccgtacc gttggctgga tcgctcactg gagcgaaatg
     1621 cacagtgacg gcatgaagat cgcccgtccg cgccagttgt acaccggcta tgaaaaacga
     1681 gactttaaat cagatgttaa acattaat
//
LOCUS       DAZKFU020000476         1705 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZKFU020000476 DAZKFU020000000
VERSION     DAZKFU020000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.478"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..174
                     /gene="tnpB"
                     /locus_tag="ACHOQQ_RS24780"
                     /old_locus_tag="pgaptmp_004956"
     CDS             <1..174
                     /gene="tnpB"
                     /locus_tag="ACHOQQ_RS24780"
                     /old_locus_tag="pgaptmp_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4473080.1"
                     /translation="ADRDGLCLFSKRLEWGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            194..>1705
                     /locus_tag="ACHOQQ_RS24785"
                     /old_locus_tag="pgaptmp_004957"
     CDS             194..>1705
                     /locus_tag="ACHOQQ_RS24785"
                     /old_locus_tag="pgaptmp_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGU4473081.1"
                     /translation="MNNTLPDDIEQLKALLIAQQAVIVRLSGEITGYAREISSLRALV
                     AKLQRMLFGRSSEKSREKIEKKIARAETRITELQNRPGEAQLQLTSMAGETAPKTSDS
                     PVRKAFPATLPRDRQVISPAETECPVCSGKLKPPGESISEQLDIINIAFRVIETVRPK
                     LACSRCDCIVQAPQPPKPIERSYASPALLARIIMAKFAEHLPLYRQSEIYARQGVELH
                     RNTMGRWVDIMGEQLRPLYDELKHYVLMPGKVHADDTPVNVLEPGQGKTRTGRLWVYV
                     RDDRNAGSTMPAAVWFSYSPERKGIHPQQHLADYRGILQADAYAGYNALYESGQVTEA
                     ACMAHARRKIHDVHVRHPTTVTGEALRRIGALYAIESEIRGSPAEERLAVRKARTVPL
                     MQSLYEWLQGQMSTLSRHSDTAKAFTYLLKQWDALNEYCRNGWVEIDNNLCENALRVV
                     ALGRRNYMFFGSDGGGESAAVMYSLIGSCKLNGIEPETWLRHVISVINTWPANR"
BASE COUNT          419 a          478 c          500 g          308 t
ORIGIN      
        1 gccgatcgtg acgggttatg cctgttctcc aaacgcctgg aatggggccg cttcgtctgg
       61 ccggtaaccc gggaagggaa agtgcacctg acgccagctc agttatccat gctactggag
      121 gggatcgcgt ggcaacatcc caaacggaca gaacggcctg gcatccggat ataacccgtg
      181 ataaaacagg ggaatgaaca acacactccc cgacgacatc gagcaactga aggccctgct
      241 gatcgcacag caggcggtta tcgtccgtct gtctggtgaa ataaccggct atgcccgcga
      301 gatcagctcg ctcagagcgc tggtcgctaa actgcagaga atgttgttcg gtcgcagcag
      361 cgagaaaagc cgcgagaaga tagaaaagaa gatcgcacgg gcagaaacgc gtataaccga
      421 gctccagaac aggcctggtg aggcgcagtt gcaactcacc tcaatggccg gagagacagc
      481 gccgaaaaca tcagactctc ccgtccgcaa agcatttccg gcaacacttc cccgtgacag
      541 gcaggttatc tccccggcag aaaccgaatg ccccgtctgc agcggcaaac tgaaaccgcc
      601 gggagaaagc atctctgaac aactggatat catcaacatc gcgttcaggg taatcgaaac
      661 ggttcgccca aaactggcct gcagccggtg cgactgtata gttcaggctc cgcagccacc
      721 aaaacccatc gagcgcagtt acgccagtcc ggctctgctg gcccgcataa tcatggcgaa
      781 gttcgccgag catctgccgc tgtaccgtca gtcggaaatc tatgcccgcc agggcgtgga
      841 gctgcaccgc aatacgatgg ggcgctgggt tgacatcatg ggagagcagc ttcgcccgct
      901 gtatgatgaa ctgaagcact atgtgctgat gccgggtaaa gtgcatgccg atgacacgcc
      961 ggtaaatgta ctggagccgg gtcagggtaa aacccgtacc ggacggctgt gggtctatgt
     1021 tcgtgacgat cgcaacgccg gttcgaccat gccggcagcg gtgtggttct catactctcc
     1081 cgagcgcaaa ggtatccacc cgcagcaaca tctggcggac tacagaggta tcctgcaggc
     1141 cgatgcatat gcgggttaca atgctcttta cgaaagcggt caggtaaccg aagcggcttg
     1201 tatggcacat gcccgacgca agatccacga tgtacatgtc cgccatccaa cgacagtaac
     1261 gggagaagca ctccgtcgta tcggggcact gtacgctatc gagtctgaga tccgcggcag
     1321 tccggcagaa gagcgactgg cggtcagaaa agccagaacg gtaccgctaa tgcagtcgtt
     1381 gtatgagtgg ctccaggggc agatgagcac gctgtcgcgt cactctgata cggcgaaagc
     1441 gttcacctat ctgctgaagc aatgggacgc cctgaacgaa tactgccgca atggctgggt
     1501 ggagatcgac aataatctgt gtgaaaacgc cctccgggta gttgcactgg ggcggcgtaa
     1561 ctacatgttc ttcggctctg atggaggagg cgagagtgcg gcagtgatgt acagcctgat
     1621 cggtagctgc aaacttaacg gaatcgagcc ggaaacgtgg ctacgccacg tgatcagtgt
     1681 catcaacacc tggccagcca accgc
//
LOCUS       DAZKFU020000477         1704 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZKFU020000477 DAZKFU020000000
VERSION     DAZKFU020000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.479"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..798)
                     /locus_tag="ACHOQQ_RS24790"
                     /old_locus_tag="pgaptmp_004958"
     CDS             complement(<1..798)
                     /locus_tag="ACHOQQ_RS24790"
                     /old_locus_tag="pgaptmp_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473082.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLTCEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKNRMAKLFNQSDTSVRNKYRPAFDKWNELV
                     GKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSN"
     gene            complement(811..1698)
                     /locus_tag="ACHOQQ_RS24795"
                     /old_locus_tag="pgaptmp_004959"
                     /pseudo
     CDS             complement(811..1698)
                     /locus_tag="ACHOQQ_RS24795"
                     /old_locus_tag="pgaptmp_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          471 a          363 c          305 g          565 t
ORIGIN      
        1 gttagagaga tgaagaactc gaatatcaaa aaggtctttt aacatcatta cttcttcggc
       61 tgttaatgaa tcgcaactaa aatatccctg ttgatcaaaa aggttttcta tttctccatc
      121 atcggcgagg cgtttttcaa agagtgtttg caacatcgag ttgccaagga caatggatat
      181 cgtgcgatct ttcgcagatt tacaccccat cacaacggca acattcgtat ccccctcagg
      241 agtttcattt atcgctgtcg ctaaaatggt ggtcaaacac gcaaacggaa ctgtatttct
      301 ttcgtgattt aatttaccta ccagttcatt ccatttatca aaagccgggc gatatttgtt
      361 gcgcacagaa gtatccgact ggttaaacaa tttagccatg cgatttttta tcatctcgat
      421 atccggcgaa ccagaaagac tttgcgcata atacggtcct gatgatgttg catacattaa
      481 agccgcgccc acacctgtcg ccatgatggg cgcagccacg gttttggcaa gggcgacgct
      541 gcgatggcta tgcgcttcac aggttaaagg aacatattcc actgactgcg gtaattcctt
      601 acatgcctcc ctcatcattg ccgtatgctt tacaaacaac ggtttttcac tgcccaaaaa
      661 aactttctgc ttaattgcac taagaaaacg taaatcaaaa atgattgttt ttggtttatt
      721 attttttggc acatttccta ttatcccgct gagaaccttt cttgctgcat ggagattttt
      781 ttgcacagca cataacacaa ttgacgaagc cttctctact gcgtcattga ttttaatttt
      841 aaaatccgca tgatatacgt tgacgggcaa tacaagtatg tctggcgcta catctttggc
      901 tgagtattta ttggtgatcg acggtcgtga atccacgaga aaacttagct gcaaattatg
      961 ctctatatta ccagctgttt ttgtggtgtg attaaacata gagatgccac ctctcattct
     1021 ttcacacata tcctcaactt tttcacttcg ttcttgtggc aatttggctt taacgttaat
     1081 ttgatgattt ttacccgtgc attccttaac tacaagggga atgttatcta aagtgccata
     1141 caggctaata acgtcaccag gatttttatc atgtaagtaa gtttgattaa tactcgaagc
     1201 attcacaaag aggtcaatat tataactgtt tataacttca cctacatcaa atgtgtagtg
     1261 cttactctgg aggctgccat ccttatttaa ttgtatatca atcaatatat ccccggactt
     1321 tgtcaccata tctttgccat aaaccatttg catagccatt tcgaggagat ttttatctaa
     1381 ctgattgcgg gcgttgagta agcgactacc aaataattta tatactgttc ccgtcattgc
     1441 cagggctgcg ctttctggta ctttgcgttt ccacagtgag gtttctctat ttttaaacag
     1501 gtcatattca ttattactcg acaaaccata tttcagttcg ttaacacaat ggaacagttg
     1561 ttcctcacct tccattttaa ggcgatgtga aaaatcattt aatagcgttt gcttcggtga
     1621 ttcattactc attagttgtt tttctaaatt aacaacagaa taactttctt gttgaatgag
     1681 attaatattg gcaaccatta cagc
//
LOCUS       DAZKFU020000478         1699 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZKFU020000478 DAZKFU020000000
VERSION     DAZKFU020000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.480"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..1215
                     /gene="ompC"
                     /locus_tag="ACHOQQ_RS24800"
                     /old_locus_tag="pgaptmp_004960"
     CDS             61..1215
                     /gene="ompC"
                     /locus_tag="ACHOQQ_RS24800"
                     /old_locus_tag="pgaptmp_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4473083.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQ
                     VQGLNGTADVLDPESGKKVGEKTVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSET
                     QNMTTFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVK
                     YIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
     gene            1425..>1699
                     /locus_tag="ACHOQQ_RS24805"
                     /old_locus_tag="pgaptmp_004961"
     CDS             1425..>1699
                     /locus_tag="ACHOQQ_RS24805"
                     /old_locus_tag="pgaptmp_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4473084.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMA"
BASE COUNT          434 a          318 c          462 g          485 t
ORIGIN      
        1 ttaatttttg ttcatgacat ctaaatgcat gaatagttca gtttaaataa ggattaaatc
       61 atgaaaaaaa tgacagtggc actttctgct gtagcagtgg cagtgatgtt tgctgcgggg
      121 gcgcaggcag cagaagttta taataaagat ggtaataaac tggatcttta cgggcgtgca
      181 accgctctgc attacttctc ggatgataaa ggtaatgacg gagaccagac ttatgctcgt
      241 ctcggcttta aaggcgaaac gcagattaat gatcaactga ccggatttgg tcagtgggaa
      301 taccagttct ctggtaacaa aacggaatct gaaggttccg cgggaaataa aacccgtctg
      361 gcatttgcag gtctgagatt tgcagatgtt ggtaccattg attacggacg taactatggt
      421 attgcttacg atgtcggatc atatactgac gtactgcctg agtttggtgg tgatggctgg
      481 acgcagaccg ataactttat gacggcccga acttccggtg tcctgactta ccgtaataca
      541 gacttcttcg gactggttga tggtctgaat tttgcggcgc agtatcaggg caaaaatgag
      601 cgtgatgacc ttcagaaggc taatggcgac gggtatggtt tctcggccag ctatgagttt
      661 gatggttttg gttttgtcgc tgcgtatacc aagtcagatc gtaccgataa gcaggttcag
      721 gggttgaacg gtactgcaga tgttcttgat cctgaatcag gtaaaaaggt tggagaaaaa
      781 acagttgact caggtagtgt ggcaaaaggt aaacatgctg aattctgggg aactggtctt
      841 aaatatgatg ccaataacct gtatctggca gcagtgtatt ctgaaaccca gaatatgacg
      901 acctttggtg atcaaggtgt tgcggataaa gctcagaata tcgaagccgt tgttcagtat
      961 cagtttgatt ttggtctgcg cccgtccctt gcctaccttc agtcccgcgg acaggatgtt
     1021 atggttggtg gtgtgaacca tggcgatcag gatctggtta aatatattga tgtcggtgcg
     1081 acttattact ttaacaagaa tatgtccacc tatgttgatt ataaaattaa cctgattgat
     1141 gaaagtgaat ttacccggaa agccggtgtt gcgacagata atatcgttgc tgtgggtatg
     1201 acttatcagt tctgattatt gctgataagt taataaatac gggccgtctg cccttacagg
     1261 cggcctgtat caatgaaaac acagttttca ttggtcactg cgatcagcaa ttgccatctg
     1321 gcatgtgctg atttaacttt ctgttattac ctttattggt tttattttaa attggacttt
     1381 tattgttcgg ggcgcgtctg cgcccctttt ttatgggcgg atatatgcag gatattcacg
     1441 aagaaagcct gaacgagtcg gttaagtcag agcagtcacc acgggtggtg ctctgggaaa
     1501 ttgacctgac ggtgcagggc ggtgagcggt attttttctg caatgagctg aatgaaaaag
     1561 gggagccggt gacctggcag gggcgtgaat atcaggcgta cccgattgag ggcagcggct
     1621 ttgagatgaa cggaaagggc agcagtgccc gcccgtcgct gacagtgtcc aatcttttcg
     1681 ggctggtcac cgggatggc
//
LOCUS       DAZKFU020000479         1692 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZKFU020000479 DAZKFU020000000
VERSION     DAZKFU020000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.481"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..412
                     /locus_tag="ACHOQQ_RS24810"
                     /old_locus_tag="pgaptmp_004962"
     CDS             <1..412
                     /locus_tag="ACHOQQ_RS24810"
                     /old_locus_tag="pgaptmp_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473085.1"
                     /translation="SSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSV
                     TGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHS
                     VAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            412..987
                     /locus_tag="ACHOQQ_RS24815"
                     /old_locus_tag="pgaptmp_004963"
     CDS             412..987
                     /locus_tag="ACHOQQ_RS24815"
                     /old_locus_tag="pgaptmp_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4473086.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTSQDIEWPALP"
     gene            complement(1085..1675)
                     /locus_tag="ACHOQQ_RS24820"
                     /old_locus_tag="pgaptmp_004964"
     CDS             complement(1085..1675)
                     /locus_tag="ACHOQQ_RS24820"
                     /old_locus_tag="pgaptmp_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4473087.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          457 a          385 c          407 g          443 t
ORIGIN      
        1 atccagtacg gatttgggga cgaaaaccac atcgtcgttt gattacggca ctaaatccac
       61 gaataacacc ggggcgcata cgcacagtgt gagcggtaca gccgcaagtg ccggaaacca
      121 tactcatagt gtcacaggcg catcagcagt cagccagtgg tcacaaaatg ggtcagtaca
      181 taaggtagtg tctgcggcca gtgtgaatac aagtgctgca ggagcacaca ctcatagtgt
      241 cagcggcacc gctgcatctg caggtgctca cgcacatact gtcggtattg gtgctcatac
      301 gcactctgtt gcgattggct cacatggaca cactatcacc gttaacgctg ctggtaacgc
      361 ggaaaacacc gtcaaaaaca tcgcatttaa ctacattgtg aggcttgcat aatggcattc
      421 agaatgagtg aacaaccacg gaccataaaa atttataatc tgctggccgg aactaatgaa
      481 tttattggtg aaggtgacgc atatattccg cctcatacag gtctgccagc aaacagtacc
      541 gatattgcac caccagatat tcctgctggc tttgtggctg ttttcaacag tgatgaggca
      601 tcgtggcatc tcgttgaaga ccatcggggt aaaacggttt atgacgtagc gtcaggggac
      661 gcgttattta tttctgaact cggtccgtta ccggaaaatg ttacctggtt atcgccggaa
      721 ggggagtttc agaagtggaa cggcacagcc tgggtgaaag atgcagaagc agaaaaactg
      781 ttccggatcc gagaggcgga agaaacaaaa aacagcctga tgcaggtagc cagtgagcat
      841 attgcgccgc ttcaggatgc tgcagatctg gaaattgcaa cggaggaaga aatctcgttg
      901 ctggaagcat ggaaaaagta tcgggtattg ctgaaccgtg ttgatacgtc aacttcacag
      961 gatattgaat ggccagcact gccgtagggt aaaacatata aattctataa ttagatgtat
     1021 ctttccattt acggcaagga agggggcttg gaagacgtaa agcatctcac accaagatta
     1081 ttttttatat gtcaggtgtc tgaagttttg ctttggctct taaaatggtt tgccgcgagg
     1141 ttttgaattc ccgggcaatg gcacttatac ttacacctga cttaattcgt tcgaatacca
     1201 cctgtttctg ttcttcattt aacacaggtg gtcgaccaaa acgtttccct gcgccccggg
     1261 ctcttactat cccggaatga gtgcgttcaa gtaaaaggtc tcgttcaaat tcagcgactg
     1321 ctgaaattac ttgcatcatc attttccctg ttggactggt caggtcaatg ccccccaatg
     1381 ctaagcaatg cactctgata cctgtttcgg tcagttgttc cactgttttc ctgatatcca
     1441 ttgcattaca accaaggcga tccagttttg tcacaatcaa ttgatcacca catttcagac
     1501 gagcaagcaa ccggttaaaa ccaggacgct cactggttgc tgctgagccg ctaatgtgtt
     1561 cttcgattat ttgctgaggt ttgattttaa aacctgcact ttcgatttcc cggcgttgat
     1621 tttcggtggt ctgatccagc gttgatatcc gacagtaagc aaaaattcga gacatagtga
     1681 gactctatac ga
//
LOCUS       DAZKFU020000480         1692 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZKFU020000480 DAZKFU020000000
VERSION     DAZKFU020000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.482"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..419
                     /locus_tag="ACHOQQ_RS24825"
                     /old_locus_tag="pgaptmp_004965"
     CDS             <1..419
                     /locus_tag="ACHOQQ_RS24825"
                     /old_locus_tag="pgaptmp_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU4473088.1"
                     /translation="RFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEVDLTLNWG
                     RISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNEEIQPECQ
                     ITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            432..695
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS24830"
                     /old_locus_tag="pgaptmp_004966"
     CDS             432..695
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS24830"
                     /old_locus_tag="pgaptmp_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4473089.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            1217..>1692
                     /locus_tag="ACHOQQ_RS24835"
                     /old_locus_tag="pgaptmp_004967"
     CDS             1217..>1692
                     /locus_tag="ACHOQQ_RS24835"
                     /old_locus_tag="pgaptmp_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4473090.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISTTSEPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG"
BASE COUNT          443 a          387 c          455 g          407 t
ORIGIN      
        1 tgcgttttgt cactgtcaca gcagaagcct tacggttcag gcaaatacag agagaatttc
       61 gtctggcact gtctgaaact gctcctgttt atacgatgac accggaagaa gtggacctca
      121 cactgaactg ggggagaatc agcaatgtgc ttccggagtt tcggggagag ggtggtgtca
      181 gagtggggcg aatatccttt aataatatat cagcgatact gggcacagtg gcggttatac
      241 tgaattgcca tcatcagggg gcacgttccg ttcgcgccgt gaatgaagag atacaaccag
      301 aatgtcagat aactggcgac aggccagtta taaggataaa caatacttta tgggaaagta
      361 ataccgcagc tgcttttctg aatcgcaggg ctcactcttt aaatacatcc ggagaataac
      421 aggagttaaa tatgaagaag atatttgtag cggctttatt tgcttttgtt tctgttaatg
      481 caatggcagc tgattgtgca aaaggtaaaa ttgagttctc taagtataat gagaatgata
      541 cattcacagt aaaagtggcc gggaaagagt actggactaa ccgctggaat ctgcaaccgc
      601 tactgcaaag cgcacagtta acaggaatga cggtaacaat caaatcaaat acctgtgcgt
      661 caggttcagg atttgctgaa gtgcagttta attaatatca gaagcattgc tggtttcgtg
      721 gtgtgcagca atgtagttac agtgtaatca atgtcacaat tcagtcagtt gaaggttgtc
      781 tgcccgactg agaatttgtt aaaaaaaatc ctgcatggtg aatccccctg agcggcgggg
      841 catatcagcg tcacaggtgt ttctgtttta cctctatcct ttctgtgcgg gttcaggtgc
      901 tgatactgaa ctcaccggga ggcacccggc accatgcatg aacggtacat agcgcataca
      961 tcagcccctc tccggagggg ctttcttgtg ggcaaaaaaa agcccgcgcc gggagacgcg
     1021 ggcggcaagg aataaacaac aaaacgtgaa gtaatatttc agctggcgaa taatacccga
     1081 cagtaatcac tctgtgcaac tgcgcggcct ttttcgtatt gcgggctgta gtctcctttc
     1141 tgccattgtc ctgtaacttc cggacttcag cccgctcctt atctgactca caacattatc
     1201 ccgaccggga ggattcatga catttaaaca ctacgatgtg gtcagggcgg cgtcgccgtc
     1261 agaccttgcg gagcgactga ctcaaaaact gaaggagggg tggcagccat ttggcagtcc
     1321 tgtcgccatc acgccttata ccctgatgca ggccattgcg gcggaaggtg atgtcaccac
     1381 acctgtgctg gtgaagccgt cggatggaga aggcacagtt atcagcacca ccagcgaacc
     1441 ggagtattac tttgttgttg ttctggcggg gcagtcaaac agcatggcat atggtgaagg
     1501 ccttccgctg ccggagacat atgaccgtcc ggacccgcgt attaagcagc tggcgcgccg
     1561 cagtacggtg acaccgggcg gtgccgcctg taagtataac gacatcattc cggcggacca
     1621 ttgtctgcat gatgtgcagg acatgagccg gctgaatcat ccgaaagcgg acctgtcaaa
     1681 ggggcagtac gg
//
LOCUS       DAZKFU020000481         1668 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZKFU020000481 DAZKFU020000000
VERSION     DAZKFU020000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.483"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..1583)
                     /gene="cadC"
                     /locus_tag="ACHOQQ_RS24840"
                     /old_locus_tag="pgaptmp_004968"
     CDS             complement(45..1583)
                     /gene="cadC"
                     /locus_tag="ACHOQQ_RS24840"
                     /old_locus_tag="pgaptmp_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGU4473091.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT          487 a          339 c          348 g          494 t
ORIGIN      
        1 actcaacaac aaatattcca gagcataaat caacccggag ttacttattc tgaagcgaga
       61 aatttgtcga gataaggtac aacataagga acagaagtct ggaatatacc attttcaatc
      121 cagtaaaggg tgtttgcccc tgggcgtaaa ttaaaggcgg tgagatatgc atcagctgct
      181 tcccggttca tccccttcat ttcataaacc ttgccaagca atacataatt tagccaggac
      241 atttcaagat caatgccagt atttatcgcc tggtaagact catctgtttt accttttacc
      301 agagcactga ccgcttttat ttgatatata atggacaggt tattcaattc cggtagtgta
      361 acaatgttat ctatttctgt gttcagtgct gctaattgtt tttcatctaa aggatgttga
      421 gaatggcgca cgatatcaac taatgctttt tctgctctcg cgtaggtaaa ttctggggat
      481 gattgaacaa tctcacctaa taattcactg gcacggttca atgatttatc atcgccatgc
      541 agtaaataat catgtgcctg ataaaaatta gttaataacg caccacgatg aggcaaaatt
      601 tgctggagcg tctcctgcat tcgttgtggc cacggttggt ttaacgcttt tgataaactc
      661 tccagtaaat cattttgaat cgccagctga ttaccgttag tgatgacata acgtttatcc
      721 agcatggttg aaccatctgc attgtctacc aattttatcg acataaagca ttgttgagca
      781 cggtattggc gctgattaac aaacgcaata gataatgttt taccggaact gctcggttca
      841 tcaatgttgt agttgatttt gtcatgcacc ataaaggtgg agaaggtgtt aagtgatgtc
      901 gccaccaaat cacccacgcc tatcgcgtaa gagagctgat acggggaact ccagctgtta
      961 caacttttat ttaccatatt aatgtcaata tcgcgtggat tgagcaaaat acgcgatttg
     1021 ctcataggaa gccgtgtatc aagagttgaa aacgctacca gtgctacaca gatacctaac
     1081 gacaacagga aaaaaaacca tacccaaaag gtagtgaatc gtttgctttt aactggggat
     1141 tgttcaggtg gcgttgcggt gttttgaatg ttaagactgt gggagggaga atctgtggca
     1201 ggaaccgcct ctggtatagg gggaggcgaa gatagcatta tttcctctcc ctcttcttcg
     1261 ctgtaccaga taaccggcac cattaattta tagccgcgct ttggtacagt agcgatatag
     1321 acaggactat cttcatcatt atcttttaat gacttacgta gttctgagat actctgcgtc
     1381 acaacatgat tggtgacaat acttctcttc cagacattat cgataagttc atccctgcta
     1441 agtacttcgc cactgtgttg agcaaagaaa accagaagat cgattaatct cggctcaagg
     1501 gtaagttgac gcccattgcg gctaatttgg tttatggacg gagtaacaag ccattcgcca
     1561 acgcgaacta caggttgttg cataatagaa actcattcga aaagggaatg atgcaatgat
     1621 aattgccaca acctattttt accatctata gatgggttta tttacata
//
LOCUS       DAZKFU020000482         1663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZKFU020000482 DAZKFU020000000
VERSION     DAZKFU020000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.484"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..789
                     /locus_tag="ACHOQQ_RS24845"
                     /old_locus_tag="pgaptmp_004969"
     CDS             <1..789
                     /locus_tag="ACHOQQ_RS24845"
                     /old_locus_tag="pgaptmp_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4473092.1"
                     /translation="LLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMG
                     KRWPTGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSC
                     IGGFMGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALV
                     PKIISGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFV
                     VLVLIGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            793..1623
                     /locus_tag="ACHOQQ_RS24850"
                     /old_locus_tag="pgaptmp_004970"
     CDS             793..1623
                     /locus_tag="ACHOQQ_RS24850"
                     /old_locus_tag="pgaptmp_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4473093.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
BASE COUNT          419 a          271 c          413 g          560 t
ORIGIN      
        1 cttctgtctt gtattgcatt tgcatgtcta tatgtttgtt tttcaggcgt cactgcaaac
       61 gctgctttaa tcacagttat ggcattgtgc tgtgcggctg tttttatcag ttttatatat
      121 cagggtatgg ggaaaagatg gccaacaggc attatgttga tgcttgtggt tatgttctgt
      181 ttcctctacg gatggccact gcaggctttt cttcctacat ggcttaaggt tgatatgcag
      241 tattcaccag aaactgttgc tctcatattt atgctggcag gttttggctc tgcagctgga
      301 agttgtatag gtggttttat gggggactgg cttggaaccc gaaaagcata tgttatatca
      361 cttcttatcg gtcagcttgt tattatcccc gtttttcttg tggaccggga ctatgtatgg
      421 ttacttggcc ttctgatctt tactcagcag gtttttggtc aggggattgg tgctctggtg
      481 ccgaaaataa tcagtggtta ttttaatgtg gagcagcgtg cggcaggact ggggtttatt
      541 tataatgtag gttcacttgg tggagcatgt gcgcctattc ttggtgcagt tgttgcgtct
      601 catacaagtc ttggtacagc aatgtgttct cttgctttta tcttaacatt tgttgttctt
      661 gtccttattg gttttgatat gccatcacgt gtacagagat ggatccatcc agaagctgcg
      721 cttgaatatg atactgtcga tggaaaacct ttttatggag caagaaaaaa gaatgttgca
      781 ggagaataac ggatgctatc agggaataaa aaaatgattc attacaggga gaatatatat
      841 tctcttgata attatgagcg ttacataact gacagttcgc tatttgagaa tactgacgat
      901 ggcataatat atacaggtat tgtgcccctt attacagttg ttgagcctgt tgtatctaat
      961 caacatagtg tgctgattat acctggaggt ggatacagga ggattgttta tgaaagagag
     1021 ggacttgatg taggcagatg gatagcaggt cagggatata cggcatttat tctttcttac
     1081 agaatgccct ccgttaatgg ttattattcc tttgcggatg cgaagaatgc cctgaacttt
     1141 atttcttact gggccggaat gtcggagaga acatttaaga gtgttggatg tatggggttt
     1201 tcggcaggtg gtcatcttgc tggatatatt actcatctcc attatgagga agagacttct
     1261 gtgcgtcctg actggatggc tctgatatat ccagttatat ctcttgacag gagtattgct
     1321 catcagggat gccggaaacg tcttctggat gcaggtgaaa gtgaatattc agccagtctt
     1381 gaaaatagtg ttacagagta tatgccacca gtctttattg tgcatgcaac agatgataag
     1441 gatgttccag ttattcactc tctgagatat tttaactcta tccgggataa gggacatcat
     1501 gcagaagctc atttttttga taagggaggt catggatttg gtgtatccgg aaaaaatgga
     1561 tatccgatag aaaaatggca aagcctgctt ctggaatggc ttgcggaaca taagtttcat
     1621 tgagaaatca gattacaagt gataaatata tttaatgatg agg
//
LOCUS       DAZKFU020000483         1650 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZKFU020000483 DAZKFU020000000
VERSION     DAZKFU020000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.485"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            188..259
                     /gene="ypdK"
                     /locus_tag="ACHOQQ_RS24855"
                     /old_locus_tag="pgaptmp_004971"
     CDS             188..259
                     /gene="ypdK"
                     /locus_tag="ACHOQQ_RS24855"
                     /old_locus_tag="pgaptmp_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGU4473094.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(324..1562)
                     /gene="alaC"
                     /locus_tag="ACHOQQ_RS24860"
                     /old_locus_tag="pgaptmp_004972"
     CDS             complement(324..1562)
                     /gene="alaC"
                     /locus_tag="ACHOQQ_RS24860"
                     /old_locus_tag="pgaptmp_004972"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGU4473095.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVSGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          382 a          450 c          418 g          400 t
ORIGIN      
        1 tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc agtttagctc
       61 agaattctgg ggtgatgccc acggtaaaat acaggacttt taaggtcgtt ttgcccggtg
      121 tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagcaatt acgcggagag
      181 atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt gggccggtac
      241 ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca tcaggctcct
      301 gtttgcattt ctgaggtcgg tgtttattcc gcgttttcgt gaatatgttt gctgctggcg
      361 ggtagtaaac cgtcggcacg gaacatcgct ttaatcccac gaatcgcctg acgaatacgg
      421 tcgcggtttt caatcagtgc aaagcgcaca tgcgtatcac catagtcgcc aaagccaatc
      481 cccggagaga cacagacctt cgcttcgtta agcaatttct tggcaaattc cagcgagccc
      541 atggcagcat acggttccgg tattttcgcc cagacataca tcgaagcctt cggcatttcg
      601 accatccagc ccgcttcatg cagcccttta accaatacat cacggcggcg tttgtactgt
      661 tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc cgccacctgc
      721 aacggcgtaa aggtgccgta atcgtgatag cttttaatac gtgccagcgc actgaccagc
      781 gttttgttgc caaccataaa gccgatacgc cagcccgcca tgttgtagct tttcgacagc
      841 gtaaagaact cgaccgccac atcgcgtgca cctgacacct gcatgattga cggcgctttc
      901 cagccatcgt agacgatatc ggcataggcc aggtcatgga ccaccagcac atcgtagcgc
      961 ttcgccagcg ccacaacctt ttcgaagaac tccagttcca cgcattgcgc ggttgggttc
     1021 gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat ggcgcgctcc
     1081 agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg agccccggca
     1141 atcaccgcgc cgtaaatatg aatggggtaa cttggattcg gcaccagcac tgtgtcaccg
     1201 tgatccagtg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat agtgacgatg
     1261 gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg ggaaatggcg
     1321 cgacgtagcc gcggaatacc gcgtgaagtg gagtaaccat gcgtgtccgg gcgctgggcc
     1381 acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt acccatactg
     1441 aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc ggtaatgtta
     1501 aaaacatagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg agtgtcagcc
     1561 ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc cacgatggtg
     1621 gctgttttga aaatagcctg attaatttct
//
LOCUS       DAZKFU020000484         1647 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZKFU020000484 DAZKFU020000000
VERSION     DAZKFU020000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.486"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1647)
                     /gene="mchF"
                     /locus_tag="ACHOQQ_RS24865"
                     /old_locus_tag="pgaptmp_004973"
     CDS             complement(<1..>1647)
                     /gene="mchF"
                     /locus_tag="ACHOQQ_RS24865"
                     /old_locus_tag="pgaptmp_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HGU4473096.1"
                     /translation="IALEVWPGSEFLAETQQTRISLRSLINSIYGIKRTLAKIFCLSV
                     VIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISAGLMFFILLRAAVSMLRAWSSLVMS
                     TLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSRFGSLDTLRATFTTCVVGAIMDSIM
                     VVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTYGYYRQISEETLVRGARASSYFMES
                     LYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKLTRMDLLFGGINTFVAACDQVAILW
                     LGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLTSFLLQLRIMSLHNERIADIALHEK
                     EEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQVFSGLNLSVAPGESVAITGASGAGK
                     TTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYHRMIACVMQDDRLFSGSIRENICGF
                     AEETDDEWMTECAKASHIHDVIMKMPMGYETLIGELGEGLSGGQKQRIFIARALYRKP
                     GILFMDEATSSLDTESERFVNAAIKKMNITRVIIAHRETTL"
BASE COUNT          476 a          435 c          306 g          430 t
ORIGIN      
        1 taaagttgtc tctctatgtg caataatcac ccgggtgata ttcatctttt ttatagcagc
       61 attcacgaaa cgttcacttt ctgtatcaag agaacttgta gcctcatcca taaataatat
      121 tccaggtttc cggtataaag ctcgggcaat gaatatacgc tgtttttgac cgccggaaag
      181 accttccccc agttcaccta ttaacgtttc ataccccatt ggcattttca ttatcacatc
      241 atgaatatga cttgctttgg cacattctgt catccattcg tcgtctgttt cttctgcaaa
      301 cccacagata ttttcacgaa ttgatcctga aaatagccgg tcgtcctgca taacacaggc
      361 tatcatacgg tgataattat ttattccaag ttgacgtata tccgtgccat taaccagtac
      421 ttttccacta tctggttcaa acagtccaca taatactttc attaatgtgg tttttccggc
      481 accggaggca ccagttatag ccacactttc tcccggagcc acagacaaat tcagaccact
      541 gaatacctgt gctgactggc tgtcataccg gtagcttaaa tcagtggttt ccagtgaaac
      601 cgggctcatg tcagcaacga tttcaatttc cggtttcttt tcttcctttt catgtagtgc
      661 aatatctgca atgcgctcat tatgcagact cattattctc agttgaagaa gaaaactggt
      721 cagcgaagca acccgatccg aaaactgccc acgaaaagaa ccaaatgcca caaacatccc
      781 tattgtcatc tgattatcga tcacaaggct tgcacccagc cataaaatcg ccacctgatc
      841 acaggcggca acaaaagtat ttatcccccc gaagagcaaa tccatcctgg ttaacttaat
      901 acctgaattg atcgcatcta ttttcaggtt aagccagtgt gttccccgaa tcccagccat
      961 accctggatt tttaccgtgg caataccata caggctttcc ataaaatagg agctggcccg
     1021 ggcccccctg acaagagttt cttccgatat ttgccggtaa tagccgtatg tcaccagacg
     1081 aataagaacg taaaccatgg taaaaccgag cactatccag gtaagatatc ctccatataa
     1141 cagcatcatc acaaaaacac ccacaaccat aatactgtcc atgattgccc caacaacaca
     1201 ggtagtaaag gtggccctca aagtgtcaag ggagccaaaa cgcgactgga tatcacctaa
     1261 tttacggcgt tcaaaaaacg ccagcggcag tctgagaaga tggttaaaca gacccgactg
     1321 ccactgtata ttgatgagcg tgctcataac cagtgaggac catgcacgca gcatactcac
     1381 cgcggccctg agcaatataa agaacatcag gccagcagaa ataagcgtca gcagccctct
     1441 gtcccccgcc ggaatcgcat gatccataac cagctgagtt cccaccggca ttaccagatt
     1501 gattgcttca attacaactg acagacagaa aattttcgcc agtgttcttt taataccgta
     1561 aatactgtta atcagtgaac ggagacttat gcgggtctgc tgggtttccg ccaggaattc
     1621 acttccgggc cagacctcaa gtgcaat
//
LOCUS       DAZKFU020000485         1631 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZKFU020000485 DAZKFU020000000
VERSION     DAZKFU020000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.487"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(272..1495)
                     /locus_tag="ACHOQQ_RS24870"
                     /old_locus_tag="pgaptmp_004974"
     CDS             complement(272..1495)
                     /locus_tag="ACHOQQ_RS24870"
                     /old_locus_tag="pgaptmp_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4473097.1"
                     /translation="MALSDVWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKVTEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGEITGEPL
                     KRPIIEPVEELIKYAISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQYAWRCLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYLAWW
                     KRVESIVNCTISDSN"
BASE COUNT          472 a          378 c          312 g          469 t
ORIGIN      
        1 ataaaaaact aactttatcc agatagtgtc cgttaagaag ttttcgttac aactaagttt
       61 ctaggtaaat catgaatcga cctatagtat caaaaacatt tggtggtaaa catctgcatt
      121 tatcaaatgg caggagcgat ttaggggcat agggatagta atgatatcga tacccgcccc
      181 ctcttatgtc aacacaatgg cgtaaactta acgctcacat ctcgctcaaa gtgtgctagg
      241 agatttggtt atgttcaggt gatacaaacc gtcagtttga atcagagata gtacaattaa
      301 caatcgattc aaccctcttc caccatgcca ggtaagcttt acgctgttct tctagataat
      361 cactcttatc ataaacttgc catacccctg gcagcttatg accaagcata atttctgcga
      421 tatgaggcgc agtaagatca gaaaaatttg ttcgagctgt acgtctcaag tcatgaagag
      481 accaatgagg gaattgatat cctaaacacc gccatgcgta ctgcattaaa ttgtaaggca
      541 gcgactgcaa tgatgtccga ccaacgggct ccctgcttcc ttccttagta aaaagcatat
      601 cggaaccatt gttcatagag atagcgtact ttataagctc ttcaaccggt tcaataatgg
      661 gccgctttag cggttcgcct gttatctccc cagtcttatg tcgttctggt ggtacagtcc
      721 atactttatt aatgaaatca aaatcgtcca cccgggcggt aattagctct gaactacggc
      781 aaccaaaatg cagcaatagt ttaatgaagg cccggtattt aggaaccatt cgagaaccat
      841 cgatcgcagc ataaaggatt ttaatttcat catgtgtcag aaaccgtttc ttctgacctt
      901 tacggatatc catatcttta cccgtgatat ccgacagcgg gcgagtttca atgagctttc
      961 tcttatacgc ccagacatgg gcctgctttg cgttaattag caatcggtct gctattgctg
     1021 gagtcttagt gctaagaggc tccaagactt ctaaccaatc atgcaatgta gctgcatcgt
     1081 gagggatatt cccgatttta gagaacaggt gcagctcaaa cgagcgaagt atctgttcag
     1141 aacctttttt attttttaca caatatgctt cataccaagc acggatcaca gactctaccg
     1201 tcatggcttc agtaactttt cgtttttcag cctgcttgac caatcgtgga ttacggtttg
     1261 actcgagttc accacgaaga cggataactt cttctctggc ctcttttaat ccagttgccg
     1321 ggtaagttcc gatatcaaga cgctcacctt tccctgccca ttgataacga tattggaaca
     1381 ctacgcgacc tttcggtgat actctgacag acagaccatc acgatcggat ttaaccaaaa
     1441 ccttatcacg ttcctttcca acgactgaac gcaaccacac atcagacagc gccattactc
     1501 accttgtaca aatcgcaaaa cacctctgct gttttgtaca ttatgtacaa gcatatgtac
     1561 agatttttga tgaaagcaag ctgatcgatt taaagttaca tgaaagagtt ttcaattaaa
     1621 gaagctacat a
//
LOCUS       DAZKFU020000486         1626 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZKFU020000486 DAZKFU020000000
VERSION     DAZKFU020000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.488"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..351
                     /locus_tag="ACHOQQ_RS24875"
                     /old_locus_tag="pgaptmp_004975"
     CDS             <1..351
                     /locus_tag="ACHOQQ_RS24875"
                     /old_locus_tag="pgaptmp_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473098.1"
                     /translation="VYGDTQRFHWRVDYSTKNKPTTASLTVNGWERDEVTGRLRQWGS
                     IEVSEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPHAIKMYTLSNPSLVSVTLITV
                     RGCYGKFSWEAIGY"
     gene            361..585
                     /locus_tag="ACHOQQ_RS24880"
                     /old_locus_tag="pgaptmp_004976"
     CDS             361..585
                     /locus_tag="ACHOQQ_RS24880"
                     /old_locus_tag="pgaptmp_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473099.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            585..1256
                     /locus_tag="ACHOQQ_RS24885"
                     /old_locus_tag="pgaptmp_004977"
     CDS             585..1256
                     /locus_tag="ACHOQQ_RS24885"
                     /old_locus_tag="pgaptmp_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4473100.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
BASE COUNT          426 a          327 c          512 g          361 t
ORIGIN      
        1 gtttacggag acacacaacg ttttcactgg cgggtggatt acagcacaaa aaacaagccc
       61 acaacagcaa gcctgacagt aaatggctgg gagcgtgatg aagtaaccgg tcgtttgcgg
      121 caatggggca gtattgaggt aagtgaggat gatggtaagc ttatgacaca gcactctatc
      181 cgttttcctg tcgcttttcc ggttgctgct ctgaatgctc aggtgtctgc agtgggagac
      241 ccccacgcca ttaagatgta taccctgtcg aatccgtcat tagtgtctgt gactctgata
      301 acagtcaggg gatgctacgg caagttttca tgggaagcga tcggatactg agggcattgt
      361 atggaacagt atgttttttc accgtcagag aatatgttct atcccctgtc cctgcggccg
      421 gtttatgagg cagcagggcg ctggccggaa gacggtattg tggtggatta tgtggtgtat
      481 aaagtctttg cagcggatgc tgcaccggca ggtatgaagc ggggcgtcgg tgcggaaaaa
      541 atgccggtct gggtgccggt atcggaggag ggcacggaaa catgatgcat atcagaaatt
      601 tttcgtatta caccccggcg gagccggatg ttgcaggcgc gatgtacctg aagtctgagg
      661 atggtcagga ctggtatgag tgtcaggcgt tgttttcgcc ggagacgctg aaggtggtgt
      721 atgacagccg gggtgtgatt acgggatacg gtaaggacac tgcgttactg tggccggtga
      781 accagagtgt ggcagaagtg ccggataccc cggagagccg gaaaattgac ctgagcgggc
      841 gctggggttt tgacggggag aaaatcacgg acctgctgac cgcggagaaa gcgcgaggga
      901 tgaagggcga tgaaattaac gcctggcgta atgcgatgga agcggctaac tacacgtttg
      961 agcacaatgg gcgtaaatgg gactacggga agtcaacgca gacgcgtctt gagccgtcgg
     1021 tggcggcagc gaaagcgggg aaactgccgg aggcgttttt ctggacggat gcggaaaaca
     1081 atgatgtgga agtgacagca gaagagctta tagcgctgag tgaagcggca gagcaggcga
     1141 tgttcaccaa agggatggaa atccacgtcc ggcagcgcac catgaagaag gagctggaaa
     1201 agctgaccag tgcggatgag atactggcat acagggttgg ctgggcacag gaataacaga
     1261 caaaaagatg ggggaccatc acctccccca tgaactgagt cgattatcta attgttgtga
     1321 agagacttta cctacacgga tgcagagtgc cacggaaaag ttaatcctga aagtgatacc
     1381 ggtctcattt ccagagcatc ctgttgatat ggataatttt tcagatttcc ggaagtgaga
     1441 gttttggtgt ttccatacca gccacagttg atgtgtttta tggcgcctgg cagtgcgatt
     1501 aagggaacag ggataatgaa aaaacggggg tatcaccacc cccggtacaa ggaagtatta
     1561 actataatgg aaacattaat gaagatcttt agtgctacgt tatatggcga ataaagactg
     1621 ccacag
//
LOCUS       DAZKFU020000487         1615 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZKFU020000487 DAZKFU020000000
VERSION     DAZKFU020000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.489"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(47..609)
                     /locus_tag="ACHOQQ_RS24890"
                     /old_locus_tag="pgaptmp_004978"
                     /pseudo
     CDS             complement(47..609)
                     /locus_tag="ACHOQQ_RS24890"
                     /old_locus_tag="pgaptmp_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(673..998)
                     /locus_tag="ACHOQQ_RS24895"
                     /old_locus_tag="pgaptmp_004979"
                     /pseudo
     CDS             complement(673..998)
                     /locus_tag="ACHOQQ_RS24895"
                     /old_locus_tag="pgaptmp_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(1138..>1615)
                     /locus_tag="ACHOQQ_RS24900"
                     /old_locus_tag="pgaptmp_004980"
     CDS             complement(1138..>1615)
                     /locus_tag="ACHOQQ_RS24900"
                     /old_locus_tag="pgaptmp_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473101.1"
                     /translation="SKLLPFRIDFAYRKDTPSFGHGCKHSMCMEIYRLLSETQTMLAG
                     YYWVMEYTQNKGLHIHFIGYLDGQRHKNSYQISRQLGDIWRRITEGDGYFHLCRAKDK
                     YPVCIDHVIHYSDKSAVDDLRYALSYLAKQDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT          437 a          383 c          342 g          453 t
ORIGIN      
        1 tgtttgattc tgtatacata ataaatcacc ttacagttac aataggtcaa aaaccgctgt
       61 agccaaagtt acgctggcct gatgctttag tgtcgagctt cgtcagataa tccagacgtg
      121 ccaataagcg ctggtactgc tctgggaaat caggatcgtt aatatccagg atgtaacgtc
      181 cattagcagg gaaatgaact aatccctggt gatcttccag ttccaaccct aaggcactgt
      241 accaggctcc ggtaattaac cctctcagtg tgtcagagcg ctcatagttt cccaggtggt
      301 aacaggcgtc tttgttaaaa aaaggcagac gtgataatga catttaaagg actgcgtata
      361 ttctttcgcc cagataatac aaaccgacgt tgcgtaggtg cgtttgcctt gttgtttttt
      421 acggtgttga tctgcattaa ttttcgcatt cagcgagtta cggaaacgag atatctctcc
      481 ggggatcagg ttagggaaac aggtaatgat atccccgttg tcaatcacag gagggtaatg
      541 gagatccccc cgtattgctg ttattctgga agtgttttta atattcgtat tgatagttct
      601 cagagatata tcacactcta gatattttaa ttaagtatca gttcgatatc ggcatattgt
      661 acactacctc aggtagatga tgagatcaga tacaggaatc aactgacggt gagcgcagtc
      721 cccacatttt atccggggct taccgcatat tcccaacagc cattcgttag ccaggcagac
      781 gaataccctt tccgctggtt ttgctttccc atcgaagggc ccatacatca tcacgcccac
      841 gaaacaaccg acgaaataac gcaactttct catccgtgga taatacgaaa acgcactgca
      901 cagggctctg cggtttacgt cgccattcaa tcccatgagc ttcaagttat gtttgccctt
      961 agtgcagcta attcatcact gtcagattta tgaactatat gcgttacctg tacctgcccc
     1021 acggggtagc accacagaag atgaagtgtc atagtcccct gcgctgagga caaagccaca
     1081 cgttaacaac aaatccacgc aataaattac gatagttcgg acataagccc ccgtaattca
     1141 gttatgtcgt ggtcgacccc ggttactttt ttcgggaagc ctgctacggc ccagaataat
     1201 accgtgttct ttctgatcct gtttagccag atagctgagg gcgtaacgta agtcgtctac
     1261 cgcggattta tcactatagt gaatcacgtg atcgatacac acagggtatt tgtctttagc
     1321 ccggcacaga tgaaaataac catccccttc cgtgatccgc ctccagatat ctcccagttg
     1381 cctggatatc tgataagagt tcttgtgacg ttgcccatcc aggtaaccga taaaatgaat
     1441 atgaagtccc ttgttttgtg tatattccat tacccagtaa tatcccgcca gcatcgtttg
     1501 ggtttcacta agtaagcggt atatttccat acacatactg tgcttacagc catgaccaaa
     1561 actgggcgtg tctttcctgt aggcaaaatc aattctgaaa ggtaacagtt tagaa
//
LOCUS       DAZKFU020000488         1611 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZKFU020000488 DAZKFU020000000
VERSION     DAZKFU020000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.490"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..360
                     /locus_tag="ACHOQQ_RS24905"
                     /old_locus_tag="pgaptmp_004981"
     CDS             151..360
                     /locus_tag="ACHOQQ_RS24905"
                     /old_locus_tag="pgaptmp_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4473102.1"
                     /translation="MSEDVPLPKANQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            complement(380..613)
                     /locus_tag="ACHOQQ_RS24910"
                     /old_locus_tag="pgaptmp_004982"
     CDS             complement(380..613)
                     /locus_tag="ACHOQQ_RS24910"
                     /old_locus_tag="pgaptmp_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473103.1"
                     /translation="MARSDYDFINLSLEHELNEWLAERGYAGLADNRNRLAEVVACKL
                     QDSFCMNVSWDALNAAYSEHPEWFSGLVSGDEN"
     gene            complement(708..1331)
                     /locus_tag="ACHOQQ_RS24915"
                     /old_locus_tag="pgaptmp_004983"
     CDS             complement(708..1331)
                     /locus_tag="ACHOQQ_RS24915"
                     /old_locus_tag="pgaptmp_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4473104.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKHFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRMVGSENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTSVEVHDNLTGTTRKIPAYHL"
     gene            complement(1420..>1611)
                     /locus_tag="ACHOQQ_RS24920"
                     /old_locus_tag="pgaptmp_004984"
     CDS             complement(1420..>1611)
                     /locus_tag="ACHOQQ_RS24920"
                     /old_locus_tag="pgaptmp_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473105.1"
                     /translation="TVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYV
                     ALKTAVQSLMPRGIATIIR"
BASE COUNT          433 a          443 c          357 g          378 t
ORIGIN      
        1 agaggagagc aataaaagac acggaaaata aagaagagag agaaaatatt tcttaaaaac
       61 agaagattaa caaaacacat caggaggcag agactcctga ttcagcaata tcacacgaaa
      121 ttttagttaa ctgctaccgt tctgaaatct atgtctgaag atgttccatt accgaaagcc
      181 aatcagcgat acagagacga tcatggtgcg cttgtcaccg tgaccagtgt tgaggaaacc
      241 cgggtggtat ttatgcggga cggctatccg catccctgca tgcggccgat gtacaacttc
      301 ctgggaaaat tcaaacccga accccggaaa gaaccccctg ccggcaatca cactgcctga
      361 tggcagcata atccacttat taattctcat ccccggagac aagccctgaa aaccactcgg
      421 gatgttcact gtatgctgcg ttcagagcat cccaggaaac gttcatacag aagctgtcct
      481 gcaatttgca ggcaaccacc tctgccaggc ggttccggtt atccgccagt ccggcataac
      541 ctctctctgc cagccactca ttcagttcat gttccagaga cagattgata aaatcgtaat
      601 cggaccttgc cattcccgtt tccttaacac atgtcttcag actcagggat aaagtataaa
      661 aatgtcgccc tgctgctgcc agagcaatat tccccctgat ggccgtatca gagatggtat
      721 gccggtattt tacgggtggt tcccgtcagg ttatcgtgta cctccacgct cgtagtcacc
      781 accggcattc cggcctttct cagcctcaaa acatcagctg caatacgctg actaccagac
      841 cagaacaggc cgtccagtgc agtcaccagt aaccccgcct ccagcgcata cttcagccgt
      901 tcacggggcg ctttcacttc ccgggcaacc tgctgatatg gcaatgatgt gttttcactc
      961 ccgaccatcc ggcgaatacg atgagacaga tgataccggt atgtatccgg cacacctgaa
     1021 aggctggcct tcaggctgta cacgcagcca aagtgtttat cgttgaacac cacatttttc
     1081 tggctgatat cccattcttc acgcagcgcg gcaatcagtt gtggtgtacg ggtaagcaac
     1141 aagcgaaaag gcagttcaaa actggtgacg taatccacat tcagcagggc aatgcgaagt
     1201 cgttcttctg gtccggcttc tgtctgccgg cactcctcca ggacatcctg ccactgcagg
     1261 cgaagacggg aagactcatt cagttctgta aagcagtatt tatccgccag atagtcaatt
     1321 cgtgtatgca tactgaagag tattccgtat aaagattcag ctggcaaaac tttatcagtc
     1381 tgtaaaaact aacggaagag tcgatatttc tcccgtccat caccggatga ttgttgcaat
     1441 accccgtggc atcagagact gaacagccgt ttttaacgca acgtattgct ctgatgtatc
     1501 aggccggaca acccgaaaac acctttccac ccggcattgt ccgccagcgc ttatcaccgg
     1561 ccaggtctgt tgcagtaaat ccgccacttg cgaacatgct tcatcaactg t
//
LOCUS       DAZKFU020000489         1608 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZKFU020000489 DAZKFU020000000
VERSION     DAZKFU020000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.491"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(154..504)
                     /locus_tag="ACHOQQ_RS24925"
                     /old_locus_tag="pgaptmp_004985"
     CDS             complement(154..504)
                     /locus_tag="ACHOQQ_RS24925"
                     /old_locus_tag="pgaptmp_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4473106.1"
                     /translation="MAVIYIAGPMTGYKDDNRLEFCLADLKLRYAGNIVLNPAVLPDG
                     LSQQQYMSICIPMLMCADAIYLLDGWEESAGARAEYAMALKLNMPVSFPENRAAGETV
                     AQLLGKSKAAQATE"
     gene            complement(495..713)
                     /locus_tag="ACHOQQ_RS24930"
                     /old_locus_tag="pgaptmp_004986"
     CDS             complement(495..713)
                     /locus_tag="ACHOQQ_RS24930"
                     /old_locus_tag="pgaptmp_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473107.1"
                     /translation="MIQESTVIRFTANGRQYEVDESLIDQGMTRQDSRNPEMHHIRLI
                     NGSHFCATNMEEVRVLTATRNAGGKAWP"
     gene            complement(710..946)
                     /locus_tag="ACHOQQ_RS24935"
                     /old_locus_tag="pgaptmp_004987"
     CDS             complement(710..946)
                     /locus_tag="ACHOQQ_RS24935"
                     /old_locus_tag="pgaptmp_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473108.1"
                     /translation="MNSERNKALLRGLRHIAHAHEETAKVAAAVGKPKVVISSSISLG
                     ITMEAIRVIQDQESEIAALKKQLNEKSAGQRGQQ"
     gene            complement(982..>1608)
                     /locus_tag="ACHOQQ_RS24940"
                     /old_locus_tag="pgaptmp_004988"
     CDS             complement(982..>1608)
                     /locus_tag="ACHOQQ_RS24940"
                     /old_locus_tag="pgaptmp_004988"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HGU4473109.1"
                     /translation="ELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGCYRKPYFPEHE
                     IRAFADKANSTRTLLIHADFKDTLNSASHLFGMGNTLCVYCDPPYLQFSGRDNFTAYG
                     KPFDEHEHVRLRAALDRLSQETGGMTSITISNSDTPETRRIYQGYRMNCIQAPRSVGA
                     RTREPAPEVIATLRQCDACGHYGGGYCPDCGPVTGDATYCVMFTPAST"
BASE COUNT          317 a          414 c          447 g          430 t
ORIGIN      
        1 catgtgatta gtaaggaatg catgattgcg cgcctctcct ttgttgagga aagtgacagt
       61 cccatcatgg agtgcatgat taatcttgcc tggaataaag cctgtgaggc ggttggcagc
      121 tcttcgcgca atcatgtttc gatctccggt cggttactca gtcgcctgtg cggcttttga
      181 tttgccaagc agctgtgcga cagtttcgcc ggccgcccga ttttccggga atgacaccgg
      241 catgttcagc ttgagtgcca ttgcgtactc agcccgtgcg ccagcggact cttcccatcc
      301 atccagtaag taaatggcat cggcacacat gagcatcgga atacaaatgc tcatgtactg
      361 ctgctggctt aatccgtcag gcagtacggc aggattaaga acaatgtttc cggcgtacct
      421 aagcttcagg tccgccaggc aaaactccag tctgttgtcg tctttgtatc cggtcatggg
      481 accggcgata taaatcacgg ccatgcttta cctcccgcgt tgcgtgttgc tgtcagcacg
      541 cgtacttctt ccatgttcgt ggcgcagaag tgagaaccgt tgataaggcg gatgtgatgc
      601 atctcaggat tgcgtgaatc ctggcgtgtc atgccctggt caatcaggct ttcgtcgact
      661 tcgtactggc ggccgttggc tgtgaaccgg atgacggttg actcctggat cattgctgac
      721 ccctttgtcc ggcagatttc tcgttgagct gctttttaag ggcggcgatt tcactttcct
      781 gatcctgaat cacccggatt gcttccatgg tgatccccag tgagatcgag gaagatataa
      841 cgacctttgg ttttccaacg gcagccgcaa ctttggctgt ttcctcgtgt gcatgggcga
      901 tgtgacgtaa cccccgcaaa agtgctttgt ttctttcgct attcatttcc tgactcctgt
      961 gttactgatt catctgttta ttcacgtact ggcaggggta aacatcacgc agtaagttgc
     1021 atctcccgtt accggtccgc agtccgggca gtagccaccg ccatagtgac cgcaggcgtc
     1081 gcactgtctc agggtggcga taacttccgg tgctggttcg cgggttcttg cgccaacgct
     1141 gcgtggtgcc tgtatgcagt tcatccggta accctgataa atgcgtctgg tttccggtgt
     1201 gtcggaattg gagatggtga tgctggtcat gccgcccgtt tcctgcgaaa ggcggtcaag
     1261 tgctgccctc aggcgaacgt gctcgtgttc atcgaatggt tttccgtagg cggtgaaatt
     1321 gtcccggcca ctgaattgca ggtaaggggg atcgcagtac acgcaaagcg tgttgcccat
     1381 gccgaacagg tgactggcag agttcagcgt atccttaaaa tctgcgtgga taagcagcgt
     1441 tcgggtgctg tttgccttgt ctgcaaatgc gcggatttca tgctcaggga aatagggctt
     1501 gcggtagcag ccgaaaggca cgttaaaatc gccttttccg ttcacccggt acaggccatt
     1561 aaaacagtgc cggttgaggt acaaaaacat ggcggccagt cgcagttc
//
LOCUS       DAZKFU020000490         1601 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZKFU020000490 DAZKFU020000000
VERSION     DAZKFU020000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.492"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(497..1588)
                     /gene="ychF"
                     /locus_tag="ACHOQQ_RS24945"
                     /old_locus_tag="pgaptmp_004989"
     CDS             complement(497..1588)
                     /gene="ychF"
                     /locus_tag="ACHOQQ_RS24945"
                     /old_locus_tag="pgaptmp_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGU4473110.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT          391 a          377 c          366 g          467 t
ORIGIN      
        1 gtatctgcat aacaaaaaca atatgcgcca tttttgcatt actaataaga aaaagcaaaa
       61 aacatcgata ttcatttttg atcaatgatt tttctggcat atcgttatta tcgtgctatt
      121 ggttatcatt ttcatcttgc caggatcgca cgtaatctca ttattcgggg actttatctg
      181 cgggatatta cccttcaggt aagtcctttt ctcgccagca tattggagcg tgatcaatat
      241 tgatcagctg tgaagggggt gatgtgtagt ggaacaaaaa cttacgttat atcactacta
      301 atgagatatc cgtcatctga acactgatgt ctttaattat tctggtggtt cactgtaggc
      361 aataagcaca aaagtgtagg atgttacaag aatgattagg actcggtgaa atgaaaaatc
      421 cacgcaattg cgtggatttt atatactttt gcgctcttca tgagatttag cgaaacctca
      481 tgagacaaca aattaattag acgttgaaaa ggaagttcat cacatcgcca tctttaacga
      541 tgtaatcttt accttctgca cgcattttgc ctgcttcttt cgcgccttgt tcacctttgt
      601 aagtgatgaa atcttcaaac gagatggttt gtgcacggat aaagcctttt tcaaaatcag
      661 tatggatttt gcccgctgcc tgcggcgcgg ttgctccaac tggaatggtc catgcacgca
      721 cttctttcac cccagcggtg aagtaagttt gcaggttcag cagcttataa ccggcacgga
      781 tcacacggtt caggcccggc tcttccagcc caagctcctg cataaactcg tcacgttctt
      841 cgtcgtccag ttcggcaatg tctgcttcaa cagcggcaca aaccggaacc acaacagaac
      901 cttctttcgc cgcgatttca cgcacctggt caagatatgg gttgttttca aaaccgtctt
      961 cgttgacgtt ggcgatgtac attgttggtt ttagcgtcag gaagctcagg taacgaatag
     1021 ccgctttctc ttcagcgctt aaatccagcg cgcgcagcat acctgcgttt tccagctggg
     1081 gcaggcattt ttccaggacc gccagctcag ctttcgcgtc tttatcgcca cctttggctt
     1141 tcttctgtac gcgatgaatc gcacgttcgc aggtgtcgag gtctgccagc gccagttcgg
     1201 tgttgataac ttcaatatcg tcagccgggt taactttgcc ggaaacgtga atgatgttgt
     1261 cattttcaaa gcagcgaaca acgtgaccga tcgcttcggt ttcacggatg ttggtcagga
     1321 actggttacc cagaccttcg cctttcgatg cgccttttac cagaccggcg atatcgacaa
     1381 attccatggt cgtgggaagc gtacgttgcg gttttacgat ttcagccagt tgatccaggc
     1441 gaggatcagg cattggtacg acgcctgtgt tcggctcaat ggtgcagaat ggaaagttgg
     1501 ccgcttcaat accggctttg gtcagcgcgt tgaacagggt agatttcccg acgttgggca
     1561 aaccgacgat accgcatttg aatcccatga tttaaatcac c
//
LOCUS       DAZKFU020000491         1601 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZKFU020000491 DAZKFU020000000
VERSION     DAZKFU020000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.493"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1077
                     /locus_tag="ACHOQQ_RS24950"
                     /old_locus_tag="pgaptmp_004990"
     CDS             <1..1077
                     /locus_tag="ACHOQQ_RS24950"
                     /old_locus_tag="pgaptmp_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGU4473111.1"
                     /translation="FAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRV
                     RVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIM
                     GRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTE
                     VLNNRTTDVINNHAETIGNNQIIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIG
                     LVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKT
                     KKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINW
                     NCAASKSPPKTPDEKQDTPDMREY"
     gene            1144..>1601
                     /locus_tag="ACHOQQ_RS24955"
                     /old_locus_tag="pgaptmp_004991"
     CDS             1144..>1601
                     /locus_tag="ACHOQQ_RS24955"
                     /old_locus_tag="pgaptmp_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4473112.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKATQPDG"
BASE COUNT          397 a          402 c          495 g          307 t
ORIGIN      
        1 tttgcggtaa taccggcaga cagaacatgg cgaccacagc cgttgctgaa accgctggtg
       61 gacggcccgc agagcgccgt cgtgacggga ccggcaggcg aggaaatctt ctgtgatgag
      121 catggccgcg tgcgggtgaa atttaactgg gaccgttata acccgtcaaa ccaggacagt
      181 tcatgctgga tccgtgtggc acaggcgtgg gcaggcaccg gattcggtaa ccttgcgata
      241 ccgcgcgtgg gtcaggaggt gattgtggac ttcctcaacg gcgatccgga ccagccgatc
      301 attatggggc gcacctacca ccaggaaaac cgcacacccg gcagcctgcc gggaacaaag
      361 acgcagatga ccattcgttc gaaaacctat aagggcagcg ggtttaatga actgaagttt
      421 gacgatgcga cagggaaaga acaggtctac atccacgcgc agaagaacat gaacaccgag
      481 gtgctgaata accgcaccac ggatgtgata aataaccatg ctgagaccat cggcaacaat
      541 cagataattg cggttaccaa caatcagata cagacggtgg gcgttaacca gatagagacg
      601 gtgggcagta accagatcat taacgtgggt tctgttcagg tggaaacgat tggacttgtt
      661 cgtgcgctga ccgtgggcgt ggcgtatcag acgacggtag gtggcattat gaacacctcg
      721 gtggctctga tgcagtcctc gcagatcggt ttgcataaat cgttgagggt cgggctgggt
      781 tatgacgtca aagtcggaaa taacgttacc ttcaccgttg gtaaaacgaa aaaggatgat
      841 accgggcaga ccgcgattta ctccgccggt gagcatctgg agctctgctg tggtaaggca
      901 aggctggtgc tgacgaagga cggacaaatt tttctcaacg gcacaaaaat tcatttgcag
      961 ggtaaggagc aggttaatgg tgactcactg ttgattaact ggaactgtgc agcctcgaaa
     1021 tccccaccga agacccctga tgaaaagcag gatacgccgg atatgagaga gtactgatcc
     1081 cgtacctgtt cggtattcag taacaaacaa gcattaaaaa taaacagaag aaggaattga
     1141 atcatgagcg gaaaaccagc ggcgcgtcag ggagatatga cccagtatgg cggtcccatt
     1201 gtccagggtt cggcaggtgt aagaattggc gcgccaactg gcgtggcgtg ctcggtgtgt
     1261 ccgggcggga tgacttcggg caacccggta aatccgctgc tgggggcgaa ggtgctgccc
     1321 ggcgagacgg accttgcgct gcccggcccg ctgccgttca ttctctcccg cacctacagc
     1381 agctaccgga cccggacgcc tgcaccggtg ggcgttttcg gccccggctg gaaagcgcct
     1441 tctgatatcc gcttacagct acgtgatgac ggactgatac tcaacgacaa cggcgggcgg
     1501 agcattcact ttgagccgct gctgccgggg gaggcggtgt acagccgcag cgagtcaatg
     1561 tggctggtgc gcggtggtaa ggcaacgcag ccggacggcc a
//
LOCUS       DAZKFU020000492         1579 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZKFU020000492 DAZKFU020000000
VERSION     DAZKFU020000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.494"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..726
                     /locus_tag="ACHOQQ_RS24960"
                     /old_locus_tag="pgaptmp_004992"
     CDS             <1..726
                     /locus_tag="ACHOQQ_RS24960"
                     /old_locus_tag="pgaptmp_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4473113.1"
                     /translation="ARTEGDATALAAARAHSDENDKRTLVSANTYADQKETAINNRTD
                     GLLTTERQARTAGDTATLAAARTHSDENNKRTLISANTYADQKETDINNRTDGLLMTE
                     RLARIAGDAETLATAEAYSDAGDSLTLKQATAYSDISTRRALSEANRYTDNKFRNLSN
                     KIERAEKRLNAGIAGVTAISSIPYVAENSFSYGIGVGNYQYANAVAAGIQYKTSRNTN
                     IRLNISLDSSHNTAIGAGVAGGW"
     gene            complement(966..1454)
                     /locus_tag="ACHOQQ_RS24965"
                     /old_locus_tag="pgaptmp_004993"
     CDS             complement(966..1454)
                     /locus_tag="ACHOQQ_RS24965"
                     /old_locus_tag="pgaptmp_004993"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4473114.1"
                     /translation="MFQLTPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHHIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
     gene            complement(1460..1579)
                     /locus_tag="ACHOQQ_RS24970"
                     /old_locus_tag="pgaptmp_004994"
     CDS             complement(1460..1579)
                     /locus_tag="ACHOQQ_RS24970"
                     /old_locus_tag="pgaptmp_004994"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGU4473115.1"
                     /translation="MRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
BASE COUNT          459 a          388 c          366 g          366 t
ORIGIN      
        1 gcccgaacag aaggtgatgc aacagcgctg gctgcagcca gagcccacag cgatgaaaac
       61 gacaaaagaa cactggtcag tgcgaatacc tatgctgacc agaaagaaac cgccatcaat
      121 aaccgtactg acggattact gacgaccgaa cgccaggcac gcaccgcggg cgatactgca
      181 actctggctg ctgccagaac gcacagcgat gaaaacaaca aaagaacact gatcagtgcg
      241 aatacctatg ctgaccagaa agaaaccgac atcaataacc gtactgacgg attactgatg
      301 accgaacgcc tggcacgcat cgcaggtgat gcagaaactc tggcaacagc agaggcatac
      361 agtgacgccg gggattcact gacactgaaa caggcaacag cctacagcga tatcagtacc
      421 cgccgggcac tgtcagaggc caatcgttat accgacaaca aattcagaaa cctgagcaat
      481 aaaattgaac gggcagaaaa aagacttaat gcaggcatcg ccggagtgac ggcaatatcc
      541 tccatcccct atgtggctga aaactctttt tcatacggga tcggcgtggg taactatcag
      601 tacgctaatg ctgtggccgc aggcattcag tataaaacat cacgtaatac caatatacgc
      661 ctcaacatct ccttggactc atctcataac accgcgattg gtgcaggggt tgccggcggc
      721 tggtaaataa tcttgcgtac atacggcatt atggtattcc gtaatcacat taattcagta
      781 cgcaacaccg ccccatccgg aacagaccac tggactgagc gggataatca aggaaaaagg
      841 gcgcgcagtg tggtcagcct tcacaacaat ccggcggcag atttcctgcc gccgtgttgc
      901 ccgttaacat ttaacttcgt gggctgacgt tgccacaagc accgtatttc cctgatttat
      961 tctgtttatt ttattgtgga taatgatgcc aatggcatga ataaagttct tccggactcc
     1021 gggaacgcca taaagccata atgtttgtaa aatgcgactg ccgtgtcatc ttttgcatca
     1081 acaataagcg catatgctgc aatttcagat gtgtatgcac gatatagcgc atcagctaaa
     1141 agtgctcccc caagccccgt tccttttgat gacagactta ctgccagtcg tcccatacga
     1201 atagccggaa tagtgggata tcgcggaagc ttttttctca aatgctcagg taagtcagtt
     1261 aataatatac tggccgctga taacgtatag tatccgatga tatgatgatc gttatctaca
     1321 gcgacaaaac aggccgcgat gcgtcgtcga atatcctgtg tgacatgctc ttttatgtaa
     1381 cggtccagag catcagagcc acataaaaat gaggttctgt catatgtgct gttaaatggt
     1441 gtcagttgaa acattatatt tattttgtgt acagcatatt attacggcgt ttaaaggccc
     1501 gtttaagcgc atcattcggc tctgttggtg acagtaatgc ctgagcaaaa atttcctgat
     1561 ctgtccggga cagtcggat
//
LOCUS       DAZKFU020000493         1570 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZKFU020000493 DAZKFU020000000
VERSION     DAZKFU020000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.495"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..454)
                     /locus_tag="ACHOQQ_RS24975"
                     /old_locus_tag="pgaptmp_004995"
     CDS             complement(<1..454)
                     /locus_tag="ACHOQQ_RS24975"
                     /old_locus_tag="pgaptmp_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HGU4473116.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGSPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAA"
     gene            complement(474..935)
                     /locus_tag="ACHOQQ_RS24980"
                     /old_locus_tag="pgaptmp_004996"
     CDS             complement(474..935)
                     /locus_tag="ACHOQQ_RS24980"
                     /old_locus_tag="pgaptmp_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGU4473117.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(963..>1570)
                     /locus_tag="ACHOQQ_RS24985"
                     /old_locus_tag="pgaptmp_004997"
     CDS             complement(963..>1570)
                     /locus_tag="ACHOQQ_RS24985"
                     /old_locus_tag="pgaptmp_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU4473118.1"
                     /translation="VDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMG
                     SGFNELKFDDATGKEQVYIHAQKDMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNR
                     LLMVEKGDKITGVRKGSLSENVFQDRGTIAGSVHVDAVDNGGEGNGIQAYTAIKEIML
                     AVEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
BASE COUNT          301 a          450 c          391 g          428 t
ORIGIN      
        1 gggctgctgg ctttccgtta atttttacgt tttcagaacc ggtattgatg gcgccgtccg
       61 tgtcacaaaa gctgtttatc catgattcaa gctggttctg tttctcctcc acataatcac
      121 tcgccttata gctggcatac atcagcgcag gcaccagaac cagcgcataa gggccggtgg
      181 cacaggcggc cataatcagg caaccttctg cgatggtcac tatcgccccc agcacggccc
      241 ctgccagcgc ccccaaaaaa ctcttgtgct gaatttcatc gcccagcctg gcggcggggg
      301 agccagactt ttttgatgcc aacatctgtg caatggcttt gtcgttggct gcccgtttca
      361 tctggctggc gaccatcgcc tgctgtgaca gcattcgcat tgccagcgta ccgcctgcgg
      421 tcgctccctg tggcccgcct ggtccttcac tcatgtgtta tccctcgttt atgtcagcgc
      481 ggctggtcct tttggatgtc attctccggt ggcaacgggg tgaagctctt tatcacctcc
      541 agcagcatcg tcttttgact gttcttcagg gataaggagg tgctgaaagt gaaggtcagc
      601 agcgtatctc cacgccgctg gaatacggct atctggctaa tccgcccctg attcccttca
      661 tcttccggac cgttccagga ataatccagc agccaggccg gggcctctgc cagagtgatt
      721 tcccggcagg catgttcggt gtacccatca agtttgccct ggtacagcgc cagcgtttcc
      781 tggtacagct ctggtgtcag ggttccctgt ggaacggctt cgcgggtaat gaccaggttg
      841 atgccactgt tatctgtgga caccaggacc tgcattgact gatcctgcca ttgagacggc
      901 agttgaatgt ggccttcatt aaaagtgaac tgcataaaat actccctgat aatctgagtg
      961 aatcagttga taaaaacaga accgcccacg atggtgatgc catccttcga cagcctgatt
     1021 accgttgatt ccccgacctg tagctgaatg ccatccggcg tcagcgcaat tttgctttcc
     1081 tccacggcca gcataatttc cttaattgcc gtataagcct gtataccgtt gccttcgcca
     1141 ccattgtcta cagcgtcaac atgcaccgaa ccggcaatcg tgcctctgtc ctgaaagaca
     1201 ttctcagata aagaaccttt tcgtactcct gtgatcttgt cgcctttttc caccataaga
     1261 aggcgattac cttcctgtat cgtgaccgtc tggtcatttt ttacggtttc gcgatgatcg
     1321 tgttttacgg tggtggtacg atcgttgagt acctcggtat ccatgtcctt ctgcgcgtgg
     1381 atgtagacct gttctttccc tgtcgcatcg tcaaacttca gctcattaaa cccgctgccc
     1441 atatacgttt tggaacggat ggtcatctgc gtcttcgtcc ccggcaggct gccgggggtg
     1501 cggttttcct ggtggtaggt gcgccccata atgatcggct ggtccggatc gccgttgagg
     1561 aagtccacta
//
LOCUS       DAZKFU020000494         1570 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZKFU020000494 DAZKFU020000000
VERSION     DAZKFU020000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.496"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1024
                     /locus_tag="ACHOQQ_RS24990"
                     /old_locus_tag="pgaptmp_004998"
     CDS             <1..1024
                     /locus_tag="ACHOQQ_RS24990"
                     /old_locus_tag="pgaptmp_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HGU4473119.1"
                     /translation="PVIKHKAGLLNLAEELSNVSKACKIMGVSRDTFYRYRELVDEGG
                     VDALINRSRRAPNLKNRTDEATEQAVVDYAVAFPAHGQHRTSNELRKQGVFISGSGVR
                     SVWLRHNLENFKKRLKALEEKVARDGIELTDSQIAALERKASDDEACGEIETAHPGYL
                     GSQDTFYVGNLKGVGRIYQQTFVDTYSKVAHCKLYVTKTPITAADLLNDRVLPFYASQ
                     GLPMLRILTDRGTEYCGKVEQHDYQLYLAINDIEHTKTKAMSPQTNGICERFHKTILN
                     EFYQVTFRKKLYGDLDTLQSDLDEWLAHYNNERTHQGKMCNGRTPMETLLDGKRIWAE
                     KNLSQM"
     gene            complement(1084..1570)
                     /locus_tag="ACHOQQ_RS24995"
                     /old_locus_tag="pgaptmp_004999"
                     /pseudo
     CDS             complement(1084..1570)
                     /locus_tag="ACHOQQ_RS24995"
                     /old_locus_tag="pgaptmp_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT          439 a          338 c          381 g          412 t
ORIGIN      
        1 tcccgttatc aaacacaaag ccggtttgct caatctggct gaagaactca gcaacgtatc
       61 aaaagcctgt aaaatcatgg gcgtttcgcg tgatacgttt tatcgttacc gcgaactggt
      121 cgatgaaggc ggtgtggatg cgctgattaa tcgtagtcgc cgcgctccta accttaagaa
      181 ccgtaccgat gaggcaactg aacaggctgt tgttgattat gccgtcgctt tcccggcaca
      241 cggtcagcac cggaccagca acgagctgcg taaacagggc gtttttatct ccggcagtgg
      301 tgtccgttct gtctggttac gccataacct tgagaatttc aaaaaacgtc tgaaagcact
      361 ggaagaaaaa gtagcccgtg atggcattga gctaactgac agccagatcg cagcactgga
      421 gcgtaaagcc agtgatgacg aggcctgtgg cgaaatcgaa acggctcatc cgggatatct
      481 gggttcgcag gacacgttct atgtaggcaa cctgaaaggt gtcgggcgta tttatcagca
      541 gacgttcgtt gatacgtact cgaaagtggc tcactgtaag ctttacgtca ccaaaacacc
      601 gattaccgcg gctgacttac tgaatgatcg tgtactgccg ttttatgcgt ctcagggcct
      661 gccgatgctg agaatactga ccgacagagg cacagagtac tgtggcaaag tagagcagca
      721 tgattatcag ctttatctgg caataaacga tatagaacac acgaaaacaa aggcgatgtc
      781 cccgcagacc aacggcatct gcgagcggtt ccataaaaca atcctgaacg aattttatca
      841 ggtaacgttc cgtaaaaaac tgtacggcga tcttgataca ttacagtcgg atcttgatga
      901 atggctggct cactataata atgagcgaac ccatcaggga aaaatgtgca atggacggac
      961 gccaatggaa acgttacttg atggaaagcg catctgggcc gagaaaaatt taagccagat
     1021 gtaatctgac agatacctgt ataaataacc ggtaactgtc agatcaggtc tgagctaata
     1081 caaattaggc atttgggcag gaagggttac aacgtatcct atcgtttggc cggtatagtc
     1141 tggagtacca taaccaggtc ccaaaaccac ttcgtgtatt gtgtatttac tgttgggttc
     1201 gaaggattcg acatcttgca ataaatattt cagttctgcg gttaacctcg cacccgcgta
     1261 agctttattg tatttctggg cgtgaatatt atctaatgta gtttcatggt tccatccaaa
     1321 agaattatca ccattcattg ttactccatc gtaagcatta cacttctttg ctaacttatt
     1381 gaatgcttgc cagtcagaga gatctagttt gtggatctca acagtacgtc tgccatcgca
     1441 attatgatct cttccacgat ttgacagtaa tgtagcgcgt gcgtattgat gaaattttag
     1501 ttttgctggt ttgtcggtgg acgaaaaacg aggtaaatat gcggggaatg aataaatagt
     1561 tgttggtaat
//
LOCUS       DAZKFU020000495         1570 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZKFU020000495 DAZKFU020000000
VERSION     DAZKFU020000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.497"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..251
                     /locus_tag="ACHOQQ_RS25000"
                     /old_locus_tag="pgaptmp_005000"
     CDS             <1..251
                     /locus_tag="ACHOQQ_RS25000"
                     /old_locus_tag="pgaptmp_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HGU4473120.1"
                     /translation="EMLQDKNEEVRIDAIIGLSYRKDKRVLSVLCDELKKNTVYDDII
                     EAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
     gene            279..1200
                     /locus_tag="ACHOQQ_RS25005"
                     /old_locus_tag="pgaptmp_005001"
                     /pseudo
     CDS             279..1200
                     /locus_tag="ACHOQQ_RS25005"
                     /old_locus_tag="pgaptmp_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1200..1526
                     /gene="ybfB"
                     /locus_tag="ACHOQQ_RS25010"
                     /old_locus_tag="pgaptmp_005002"
     CDS             1200..1526
                     /gene="ybfB"
                     /locus_tag="ACHOQQ_RS25010"
                     /old_locus_tag="pgaptmp_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HGU4473121.1"
                     /translation="MKYIIFSFRAIWLALSLLMLFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVADIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
BASE COUNT          456 a          279 c          394 g          441 t
ORIGIN      
        1 tggagatgct tcaggataaa aatgaagaag tccgtattga cgcaataatc ggactttcct
       61 acagaaaaga taaaagggtt ttatctgttt tatgcgatga gttaaaaaaa aatactgttt
      121 atgatgatat cattgaagct gcgggtgaat taggtgataa aacgctactt cctgttttag
      181 atactatgtt gtacaagttt gatgacaatg aaattataac ttccgctatt gataagctga
      241 agcgttcata gcgtgagttg cctatgcaca gtgggggatt cccgccggca cggtgcagat
      301 gctcgaccgg ctggaaagtg aaatcctggc tgagcgggtg agtgaggaaa gccgccgctg
      361 gctggcatcg tgcggcctga cggtggagca gatgcaaaac cagatggacc cggtgtacac
      421 gccggcgcga aaaatccacc tgtaccactg cgaccatcgc ggcctgccgc tggcgcttat
      481 cagtgcggaa ggggctacag aatggtgcgc agaatacgat gaatggggca acctgctgaa
      541 tgaagagaac ccgcaccagc tgcagcagct tatccgcctg ccggggcagc agtatgatga
      601 ggagtccggc ctgtattaca accgccaccg ctattatgac ccgctgcagg ggcggtatat
      661 cactcaggat ccgattgggc tgaagggggg atggaatttt tatcagtatc cgttgaatcc
      721 gatctcaaat atagatccat taggattaga aacactaaaa tgcattaaac cactgcattc
      781 aatgggcgga actggtgaaa gaagcggtcc agatatatgg gggaatccgt tctatcatca
      841 atatctttgt gtcccagatg gtaaagggga ctatacttgt ggtggccaag accaacgggg
      901 agaatcaaaa ggagatggtc tatgggggcc aggtaaagca agtaatgata caaaagaagc
      961 tgctggccgt tgtgacctcg ttgaaaccga taatagttgt gtggagaact gtttaaaagg
     1021 gaagtttaaa gaggtaaggc gcgttattct gtattgcctg atatattcac acctataaat
     1081 ttagggctat ttaaaaactg ccaagactgg tctaatgatt ctttagaaac atgtaagatg
     1141 aagtgctccg gaaataacat tggacgcttt attagatttg tattcaccgg agtgatgtaa
     1201 tgaaatatat tattttttca ttcagagcta tatggctggc gttatcactt ttaatgctat
     1261 tcttttccat gcatagactt tcactattag actccactcg tgatgtgagc gagttaatta
     1321 gtctcatgtc ttatggaatg atggtaatat gttttccaac aggcatagta ttctttattg
     1381 ctctaatatt tatagggact gttgcagaca ttattggcgt aaggattgat agtaagtata
     1441 ttatggcgat aataatatgg ctttattttc tgtcaggagg gtatattcaa tggtttgtat
     1501 taagtaagcg cattataaac aaataaatta agttaattgc tcttattatt atatgtaacc
     1561 tggacattga
//
LOCUS       DAZKFU020000496         1565 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZKFU020000496 DAZKFU020000000
VERSION     DAZKFU020000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.498"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..1487
                     /gene="gndA"
                     /locus_tag="ACHOQQ_RS25015"
                     /old_locus_tag="pgaptmp_005003"
     CDS             81..1487
                     /gene="gndA"
                     /locus_tag="ACHOQQ_RS25015"
                     /old_locus_tag="pgaptmp_005003"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGU4473122.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     DNPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGQPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAGNPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLN"
BASE COUNT          395 a          372 c          390 g          408 t
ORIGIN      
        1 tcgttattgt acattatagt taatttcttt aatatctagc gatatatcaa ttaatgacta
       61 acccctgaca ggagtaaaca atgtcaaagc aacagattgg cgtcgtcggt atggcagtga
      121 tggggcgcaa ccttgcgctc aatatcgaaa gccgtggtta taccgtctct attttcaacc
      181 gttcccgtga aaagacggaa gaagtgattg ccgacaatcc aggcaagaaa ctggttcctt
      241 actatacggt gaaagagttt gttgaatctc tggaaacgcc tcgtcgcatc ctgttaatgg
      301 tgaaagcagg tgcaggcacg gatgctgcta ttgattctct caagccatac ctcgataaag
      361 gcgacatcat cattgatggt ggtaacacct tcttccagga caccatccgt cgtaaccgtg
      421 agctttctgc agaaggcttt aacttcatcg gtaccggtgt ttccggaggc gaagaaggtg
      481 cgctgaaagg tccttccatt atgcctggcg ggcagaaaga agcctatgaa ctggttgctc
      541 cgatcctgac caaaatcgcc gccgttgctg aagatggcca accgtgtgtt acctatattg
      601 gtgccgatgg cgcgggtcac tatgtgaaga tggttcacaa cggtattgaa tacggtgata
      661 tgcaactgat tgctgaagcc tattctctcc tgaaaggcgg cctgaatctc tctaacgaag
      721 aactggcaca gacctttacc gagtggaata acggtgaact gagtagctac ctgattgaca
      781 tcaccaaaga tatcttcact aaaaaagatg aagatggtaa ctatctggtt gatgtgattc
      841 tggatgaagc agctaacaaa ggcaccggta aatggaccag ccagagtgcg ctggatctcg
      901 gcgagccgct gtcgttgatt accgagtctg tgtttgctcg ttatatctct tctctgaaag
      961 agcagcgtgt tgccgcgtct aaagttctgt ctggtccgca agcgcagcca gcaggcgaca
     1021 aggctgagtt catcgaaaaa gttcgtcgtg cgctgtatct gggcaaaatc gtttcttacg
     1081 ctcagggctt ctcgcagctg cgtgctgcgt ctgaagagta caactgggat ctgaactacg
     1141 gcgaaatcgc gaagattttc cgtgctggtt gcatcatccg tgcgcagttc ctgcagaaaa
     1201 tcaccgatgc atatgccgga aatccgcaga tcgctaacct gctgctggct ccgtacttca
     1261 aacaaattgc cgatgactac cagcaggcgc tgcgtgacgt cgttgcttat gcagtacaga
     1321 acggtatccc ggttccgacc ttcgccgctg cggttgccta ttacgatagc taccgtgccg
     1381 ctgttcttcc tgcgaacttg atccaggcac agcgtgacta tttcggtgca catacttata
     1441 agcgcattga taaagaaggt gtgttccata ccgaatggct gaactaatct taatttagat
     1501 atctcgcccg gattacaccg ggcttttcaa tgcctataga aaaccatcag ataggcttgt
     1561 agttt
//
LOCUS       DAZKFU020000497         1560 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZKFU020000497 DAZKFU020000000
VERSION     DAZKFU020000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.499"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(256..>1560)
                     /locus_tag="ACHOQQ_RS25020"
                     /old_locus_tag="pgaptmp_005004"
     CDS             complement(256..>1560)
                     /locus_tag="ACHOQQ_RS25020"
                     /old_locus_tag="pgaptmp_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HGU4473123.1"
                     /translation="QNANIPLKEASSQSEEPHQLVNSKSEGNLHNSASKDNNIGAMLK
                     KDVEKLSTLVSERSPRNEEWKCQHDIQLNFVKNMLRNFPTENLSEKGSALLHESYLLI
                     KNVLQLTENVNTIDGKLKDPAKILTLHNAVRKTLANVAHNTTGPGAMATTKGTLTHVI
                     ASLDSFAPEMRSSATSSTLTASSSSEFKSDKYICSDIADLMSVLGSDYLEIYANSVEI
                     MSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHFYYIDKYPNGDFHYHY
                     NPDYVLYPPAPADKIGVPLEEAEKWCAALGLPVIPPDPKHRTPSPIVEVEPQGSGLYV
                     IIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAEYQAWLAGYNGQAENM
                     KTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
BASE COUNT          413 a          301 c          314 g          532 t
ORIGIN      
        1 aagtcaccaa atatatattg ttgtgtatta ttttttccat gttgcagttg catagaacgc
       61 tccttccatt ttctctattc atagtattaa tttgatctgt ctaaaataaa gtattggcag
      121 aattgctcta tcaaaaaaaa ctcattttgt gagattgtga tgaagaaatg aatgaaagct
      181 gatgcttaat aatatgaaac actggtccca ttttacggga ccagatagtg gacgataatt
      241 gtaggtgtta cttaatcaga aacgtaaata atctttcgct gaatcaaaca acgcagtaat
      301 ggttgaagat aataatttta taattgtatc gtaggtggag ttggctgtac tgtatttagt
      361 cgttataacc tgcacgtcgg ttttcatgtt ttctgcctgg ccattataac ccgccaacca
      421 tgcctggtat tcagccgtgc ttatttcgta gccggtgaat tctttagata tatttttttc
      481 cttcccttta tcatccctgt gaaccattga gtctgaagac tggctcatac tgtcaataat
      541 ctgagggttg ggaataataa catacagacc gctaccttgc ggttcaactt cgactatagg
      601 agaaggtgtg cggtgttttg ggtcgggtgg aataaccgga agtcctaaag cggcgcacca
      661 tttttctgct tcttccagtg gaacaccaat tttgtcagca ggtgccggag ggtataaaac
      721 atagtctgga ttgtaatgat aatgaaaatc accattagga tatttatcga tataataaaa
      781 atgcatcaag gctttttgga atgcattgac atcaaaaact atcgcatcgc cttttttatt
      841 agagtgagtc cattttccca tattgctttg gatatgttca ctgaagtcct gccagtatgc
      901 gctcattatc tcaacgctat ttgcatagat ttcgaggtag tcactaccga gcacactcat
      961 caaatcagca atatcactac atatatattt atcactttta aattcgcttg aactactggc
     1021 cgttaaagta ctgctggttg ctgaacttct catttcaggc gcaaaactat ctaaagaagc
     1081 gataacgtga gttaaggttc ctttggtcgt tgccattgca cctggcccgg ttgtgttgtg
     1141 agcaacgtta gccagggttt ttctcacagc gttatgcagg gtcaatattt tagcgggatc
     1201 ctttaatttt ccgtcaatag tattaacatt ttctgtaagc tgcagaacgt tttttatcag
     1261 caaataactt tcgtgtaaga gcgcagatcc tttctctgac aggttctcag ttgggaaatt
     1321 tctgagcatg tttttaacga agttaagttg tatgtcgtgc tggcatttcc attcttcatt
     1381 tcgcgggcta cgttcactga ccaaagttga tagtttctca acatctttct tgagcatagc
     1441 gccaatatta ttatccttac tggcgctgtt atgcaaattt ccctcgcttt tgctgtttac
     1501 cagttgatga ggctcctcac tttgcgagga tgcctctttg agaggaatgt ttgcattctg
//
LOCUS       DAZKFU020000498         1557 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZKFU020000498 DAZKFU020000000
VERSION     DAZKFU020000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.500"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(55..459)
                     /locus_tag="ACHOQQ_RS25025"
                     /old_locus_tag="pgaptmp_005005"
     CDS             complement(55..459)
                     /locus_tag="ACHOQQ_RS25025"
                     /old_locus_tag="pgaptmp_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HGU4473124.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(680..1411)
                     /gene="bluR"
                     /locus_tag="ACHOQQ_RS25030"
                     /old_locus_tag="pgaptmp_005006"
     CDS             complement(680..1411)
                     /gene="bluR"
                     /locus_tag="ACHOQQ_RS25030"
                     /old_locus_tag="pgaptmp_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HGU4473125.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          428 a          337 c          326 g          466 t
ORIGIN      
        1 attatgctaa tgccttactg aattatgaag catttcttaa gcatccaact ttagctagat
       61 taatggttta ttattttcta catcttcaat atataaaagc gtattatcaa tggcgtagta
      121 actgcgtttg ttatgattaa catcagtaac ccaacggaaa acgcccgcgc ctgccagtgt
      181 tgaacagtat tcccgaaatg tagattttcc gcaaatatga agcaatgcgg cctcttttat
      241 tttagcaggg ttcttggtcg tactaacttt taacaggttc ctggttcctc ttaataacaa
      301 aaccgtgtca tcgtgagtaa taattctgat gttatccgta gccagataat aaatgtaatg
      361 tgcaatacgg tgatgtttta attctgaata aaaccaggag aagttttgct cttttctcac
      421 ttgctcaaac atcttttgaa aaacaacgac ctgatccatc aggataataa cctcttgtta
      481 gttgtgagac tgcgtagtgt gcacgatcgg ttttaccact tcaatctggt ctgtcctttg
      541 gctgtgatat gtacaaagtg tgaaaggggg attacctgaa ttctcccggt gagcattttg
      601 taatggacca gctccggtac aaacgctgtt gtgggttcag attataacat tctgtctaag
      661 gggcgggata aaggtgaaat tagggggcat gaaagatgac tttataacct tgctcacccc
      721 agtgttgtaa aagttcgttt tgccttctcg ttggtgccat gcctgtccag acaatcaatg
      781 tttgcgtcgg gaacagttcc gggcgcggtg attcaatggg atcagcaaga acagaaatat
      841 gccatcctga caatgataaa cgccatgctt ccagccacaa tcgagctctc tcttcaacat
      901 tccacgccat cagaatggct tctttaccgg gcttacggcg catttcgaaa agcgaggctg
      961 ctgcatattc aattaatgcg ccgtcaaaca tactgctcat aatgcgggag gtgttgtgat
     1021 caagcacgag acgctggcga acaggaaggt aaacatgatt aatcaattga tctactgggt
     1081 actctcgacc cagtgaaata attctcgcgc gtagtttggc aggattagcc atacgaagaa
     1141 ttgacatcat ctcttcttgc aggcggctcc agtcatcttc cgtatcctgg ctggtggttt
     1201 ccagtaatgc tttaactttg cctacaggga cgccattact tatccaacgc ttgatctctt
     1261 cgatacgttg tatgtcttct tcatcaaaga gtcggtgtcc gccttcactg cgctgtggtt
     1321 ttaacaaacc gtagcggcgt tgccaggccc ggagagtgac aggattaatc ccgcaacgtt
     1381 cagcaacatc accaatgctg taataagcca caattcctcc ttgcggtcac aaatctccgt
     1441 cgcctgtaaa cgacccaata atactttgta caatatacgc taaaattgta caaagtataa
     1501 ataagatttc agctaatttg gatgaaacat tatttttaat gtggattaaa tttaaac
//
LOCUS       DAZKFU020000499         1540 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZKFU020000499 DAZKFU020000000
VERSION     DAZKFU020000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.501"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(567..764)
                     /locus_tag="ACHOQQ_RS25035"
                     /old_locus_tag="pgaptmp_005007"
     CDS             complement(567..764)
                     /locus_tag="ACHOQQ_RS25035"
                     /old_locus_tag="pgaptmp_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4473126.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(830..1094)
                     /locus_tag="ACHOQQ_RS25040"
                     /old_locus_tag="pgaptmp_005008"
                     /pseudo
     CDS             complement(830..1094)
                     /locus_tag="ACHOQQ_RS25040"
                     /old_locus_tag="pgaptmp_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991605.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1150..>1540)
                     /locus_tag="ACHOQQ_RS25045"
                     /old_locus_tag="pgaptmp_005009"
     CDS             complement(1150..>1540)
                     /locus_tag="ACHOQQ_RS25045"
                     /old_locus_tag="pgaptmp_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGU4473127.1"
                     /translation="ELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLMV
                     LLLNNDVWCKSREVVVLRNLIEQAWGKVLSEDNSITHLVVYPEVLSLVFDAANTYNAE
                     PGVLEQTARAYIESYGAIERHLGCSDF"
BASE COUNT          429 a          380 c          344 g          387 t
ORIGIN      
        1 tttgcaggac agttatgcta aactagcgga atcagtatca caacaataaa tgactgttca
       61 tacctgaaat ttgaattaac aggcatcatg aatgtcaata gtctaagcgg tgactactta
      121 tatcgcttgc ataaatacta aacactgact agaaaagtat tggataaccc cgtcactaaa
      181 agtaacgctt cggttatttg ctcggaatgt attaacgcca agattgttag ccgttacgcc
      241 ttatatcact ggcgctgaca aatgtttctt ttctaattta ctgaaccggc gcagacacgc
      301 aggaaagcac gccggcaccg gtgtaatgcc agttctaatc aataagaatg gaatgacgaa
      361 tcagtcagga agggataaca acccgctgct gaacggaagg tatcagcagg aagatgaacg
      421 ggaatatcgc cagcaccaag ggcagggccg ggttccacaa taccagcgct ggcattttgc
      481 acattgccag cgtaccggca ttacaacacg tttcatgctg ctgcccccag gattcctcga
      541 ttcgttgcta catccactgc tctacttcac ttccactgct ccacttcact tttgtaccag
      601 cggctgctgc gccccagttt gatgggttta gggaaatggc cttcactgat caacttgtaa
      661 aaccatttgt cggtcatacc ggtgaaactg gtgatatagt tcatgtcgat cagggaatta
      721 tcggcacgca gaaatacgct gtcgtggctt gttgtagtca acatggtcag aatgtcctgt
      781 gagatttatg aagattgtgc gaatgcgggg aatctgctga gctgtgcttt cagaactggc
      841 ctgttacggg ataccaggga ttaccggcgg ggtaacgggc ttccggatca tacacaccac
      901 gattatcgcg gacaaaatca ctgaacgccc atatcacctc tttaagtatg tcttcgcagt
      961 ccggtaaatg acgatccagc gccacatccc gggtggtaca gctttgatgc gcccggtgac
     1021 acaaagcccg gattgttcca gacatcctga atcaaacgcc ccagattagg ggcgtcgaaa
     1081 tatgcctctc tgaccattat attccggcgt acaggtagca ggtctgaagt gacaatgcgt
     1141 cacctgacgt taaaagtcac tacacccaag atgacgttca atggcaccat aggattcaat
     1201 gtaagcccgg gccgtctgtt ccagtacacc aggctcagcg ttgtatgtgt tagctgcatc
     1261 aaataccaac gacaacactt caggatacac aaccagatgt gtaatggagt tatcttcact
     1321 caatactttt ccccacgcct gctcaatcag atttcttaga accaccacct cacgactctt
     1381 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggtat cgttagccac
     1441 agcttcccta ctccagagat aatataaagg ggtgggctca acagattgat ctttacgtcg
     1501 cttacactgc aaatattcag aaatgagtct atgcagttca
//
LOCUS       DAZKFU020000500         1539 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZKFU020000500 DAZKFU020000000
VERSION     DAZKFU020000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.502"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..1539)
                     /locus_tag="ACHOQQ_RS25050"
                     /old_locus_tag="pgaptmp_005010"
                     /pseudo
     CDS             complement(1..1539)
                     /locus_tag="ACHOQQ_RS25050"
                     /old_locus_tag="pgaptmp_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          248 a          523 c          418 g          350 t
ORIGIN      
        1 aaaataatca gagacagagg ctcccatctc atccccagtt cccgaactca ttaatccata
       61 tcgatttgcc gccgcagcat gcccaaaagg attatcagga acggggccgt tacctattat
      121 catagtacta agccctaatg gatcaacgtg ttgcactgga ttcagcggat attggtacag
      181 attccatccc cccttcagcc caatcgggtc ctggatgata taccgcccct gcaacggatc
      241 gtagtaccgg tgacggttat aatacagccc cgactcctca tcgtgttgct gccctggcag
      301 acggtacggc tggtgcaggt gatgcgggtt ttcctcattc agcaggtttc cccactcatc
      361 atactccccg cttcacgcag tattgccgtc ttcgctgatg agcgccagcg gcaggccccg
      421 gtggtcgcag tggtaaaaat gaacttttcg cgccggtgtg tattccggct ccacctgtct
      481 ggccagttgc tccaccgtca gcccgcactg cgcaagccac gcccggcttt cactgctcac
      541 gcggtctgcc cggatttctt cctccagtct gtccagcagc cgcaccagtt cagccggaaa
      601 taccacgccg tgcccgtcct cgctcccttc ctgctgcatc ttctccgcca ggctgcggcg
      661 ctgcgctttc tcccgctcgc cgttctctgt ctcgacccgg atgagcggcg tgaagcttcc
      721 cggctcgtat accgtctgga tacgtgtggt gtcagtctgc accgtcgtca gcctgtcgcc
      781 gtcccacccg taccatgtct cctccggttt acgcgacagc gacatccatc ccgtcaggtc
      841 acgcccgcga cgccacaccc gtttcgccat tcgccgtccc agcgggtcgt agaggtagcg
      901 gctctcgacc agtggctcgc catgctgtat ccgcgtgtag aacaccagcc ggtgctggct
      961 gtcgtagtga tagtggtggg tgcgctcgtc gtgcatccgg ataacccctt ccgggatacg
     1021 gtccgtcttc tccgccagcc tgccgtattc atcgtagcga tagacatagt gcgcatcttc
     1081 cgcgatgcgg ttatccggcc acgccgtgaa ggtactgtcc gggtgcagtt ccgggtccgg
     1141 cagccggttt cccgccgggt ctgttgcata cgggatatcg atatccaggt tcgccgccgt
     1201 ggtgtggacg cccgtcagcc ttcccgtgtc actgtaacgg tactcccggc tctcctgcgg
     1261 gccgctgatg cggattagct gtccgttgtc gttccagtcg tagtcacggt caagctgcgg
     1321 aaggttcagg tgcctgctct ggagctggcc gctggtattc caggcggtgg caagttcata
     1381 tgcctccccg ccgaagctgc gggccgtctc acggtgcagc cggtcccgcg tgtactcgac
     1441 cagcggcgtt ccgcccagct tcatccccgc aagataaccg ctgccatacg tcagccactc
     1501 caccggcggc agaccgtccg gctcctgacg ggtcgccag
//
LOCUS       DAZKFU020000501         1539 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZKFU020000501 DAZKFU020000000
VERSION     DAZKFU020000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.503"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            126..>1539
                     /locus_tag="ACHOQQ_RS25055"
                     /old_locus_tag="pgaptmp_005011"
     CDS             126..>1539
                     /locus_tag="ACHOQQ_RS25055"
                     /old_locus_tag="pgaptmp_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4473128.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSTTPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLN"
BASE COUNT          400 a          350 c          352 g          437 t
ORIGIN      
        1 agatggagaa aattttatct tcggcgtgga tatttcttat gctctctacc atgctctctg
       61 tagatgccag cgcgagcgaa tgaatggcat tttcatgtat taaacccttt ggccgggaca
      121 agcaaatgcg caatacactc atacccatcc tcgttgcgat atgcttgttt attaccggtg
      181 tcgctatttt gaacatccag ctctggtatt ccgcaaaagc agagtacctg gcgggagcaa
      241 gatatgccgc caacaatatc aatcatatac ttgaagaagc gtcacaggcg actcaaacag
      301 cggttaacat tgccggtaag gaatgcgacc ttgaggagca atatcagctt ggcactgaag
      361 cagctctgaa acctcacctg cgcacaatca tcattctcaa acagggaata gtctggtgta
      421 catccctgcc tgggaatcgg gtcctgctgt ctcgtattcc tgttgtcccg gacagtaatt
      481 tactgttggc tccggcaatc gacaccgtta atagattacc tatcctgctc tatcagagcc
      541 aatttgcaga tacgcgcatt ttggttacga taagtgatca acatattcgc ggggcgctta
      601 atgtaccctt gaaaggggta aggtatgtat tacgcgtggc ggatgacatt attggaccaa
      661 cgggtgatgt gatgacgctt aatggacatt atccctatac cgagaaggtt cactccacaa
      721 aatatcattt cactattatc tttaacccgc cgccactctt tagcttctac agacttatcg
      781 ataaaggctt tgggatttta atatttattc tgttaatcgc ctgcgccgct gccttcctgc
      841 ttgatagata tttcaataaa agcacaacgc ctgaagagat cctgcgacgg gcaataaata
      901 atggggagat cgtccctttt taccaacctg tggtaaatgg tcgggaaggg acattgcggg
      961 gagttgaggt gttagcccgc tggaaacaac ctcacggtgg atatatttca cccgcggcat
     1021 ttattccact tgctgaaaaa tccggattaa tcgttccgct tacacaaagc ctgatgaatc
     1081 aggttgccag acagatgaac gctatcgcga gtaaactgcc ggaaggtttt catattggaa
     1141 ttaattttag cgcttcgcat attatttcgc cgacgtttgt cgacgagtgt ttaaatttcc
     1201 gtgacagttt tacccgccgc gatttaaacc ttgttctgga agtcaccgag cgtgagccat
     1261 tgaatgttga tgaaagtctg gttcagcggt tgaacattct gcatgaaaat ggttttgtca
     1321 tcgcgctgga tgatttcggt actggctact cagggctttc ttatctgcat gacctgcata
     1381 ttgattatat caaaattgat cacagtttcg ttggccgcgt caacgcagac ccagaatcaa
     1441 cccgaattct ggattgtgta ttggatctgg cgcgtaaact ttcgatcagt atcgtcgctg
     1501 aaggtgtcga aacgaaagaa caacttgact atctgaacc
//
LOCUS       DAZKFU020000502         1535 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZKFU020000502 DAZKFU020000000
VERSION     DAZKFU020000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.504"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(178..1116)
                     /gene="lrhA"
                     /locus_tag="ACHOQQ_RS25060"
                     /old_locus_tag="pgaptmp_005012"
     CDS             complement(178..1116)
                     /gene="lrhA"
                     /locus_tag="ACHOQQ_RS25060"
                     /old_locus_tag="pgaptmp_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGU4473129.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT          418 a          369 c          351 g          397 t
ORIGIN      
        1 aggcgattat ttgtgcgtac ggggcgtgaa tctctggtga ttgaaaaatg aacagttttg
       61 tacgttctgc acgatgtgaa gaaggttctt tactaaaaaa aagccgctgg ggtttaaaac
      121 acccccagcg gctcgttttt tacactattg tctcaggaat tatctatggt ccgtcgatta
      181 ctcgatatcc ctttcaatca acaatgaatc atccccttcc ggagcagata ttgggctgta
      241 ttgccacgga ttatggtagc tctccatcgc ctgatagatc acctgggcca gctcattatt
      301 actggacggg tcatagcaca gcaaatattc ggtatcaggt agcggcggta agccatctac
      361 tccactcagc acacgcaagt ccggactcat catctcgacc ggccttgccg tcacgccaag
      421 accggctttc actgccgcac gaacggccgg aagcgtcgag gcgacataag ccagtcgcca
      481 gggaatatct gctttattaa gcgtcgccag caccatatcg cgaaacggac ttggatcatc
      541 cagcaataca agagggatcg gctcaccttt ttgcaatacg tattccgctg cgcagtacca
      601 gtgtgttggc gaggtacgca ggttcaatgc cttaaacgct gaaggacgat gggtggttac
      661 catcaaatcc acttcctggg attccagcat ttctgccata taggcattac gtttaacgcg
      721 gacatccagc gctaatttcg gataaaccga actcacgcga tttaacagga aaggtaagat
      781 cgtatcggca gattcatctg aagcaccgat agttaacacg ccctgaagat tactgaacat
      841 taatgatgag caggcctcat cattaaaacg caggattttc ctggcgtaac caagaagttg
      901 aatgccatgt tcagttaaca gtttgttgcg accgtgacga gcgaacagtt ctttcccaac
      961 gagttgttcc agacgctgca tttgctgact tacggcggac tgagtacgac acacagcggc
     1021 agctgcggca gcaaaagtgt tcagatcggc aacagcaaca aatgttctca gcagatcgag
     1081 gtcgaggtta attatcggac gatttgcact tatcatatat tatcacttac tggcggctca
     1141 tactgagctg gttaatgctg tgcacacaca aacaagcaat tccatttgta atgtgcctcc
     1201 ctggcagttt catccggaac ccggacgaaa gtaaaaatgc atatgagttg cactaaaaaa
     1261 gcgactcaca ttgttccgtt ataatgcctg aagtagatca cagaatatat cttcagggat
     1321 cgcatatcta ttaagttact cactcttttc tatttatgac atgcgcgtgt ttgtataaat
     1381 gtaaatgtga gtccttgttc cactctcgtg cagcatcgct ggtcatacgc gaacacgtac
     1441 aaacagcaat ggtgaggcgg catcaagagc agggatccgt catttatgcg agcattttac
     1501 cccaaaaacc ttttatttat aagggtcatt gcgaa
//
LOCUS       DAZKFU020000503         1531 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZKFU020000503 DAZKFU020000000
VERSION     DAZKFU020000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.505"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..1476
                     /gene="iolT1"
                     /locus_tag="ACHOQQ_RS25065"
                     /old_locus_tag="pgaptmp_005013"
     CDS             43..1476
                     /gene="iolT1"
                     /locus_tag="ACHOQQ_RS25065"
                     /old_locus_tag="pgaptmp_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol import MFS transporter IolT1"
                     /protein_id="HGU4473130.1"
                     /translation="MPISVNRYNTGYILRICSIAALGGILFGYDTAVISGAISSLTSY
                     FHLSPAETGWAVSSVVVGCVVGSFSSGYLSKRFGRKKSLMISALLFTISAVGTSLSYT
                     FLHFIIYRIVGGLAVGLAATVSPMYMSEVSPKDMRGRALSMQQFAIVFGQILIFYVNY
                     KIASIATNTWLIEIGWRYMFAAGIAPCILFCILVFFIPESPRWMMMAGREEETLKILT
                     KISNEEHARHLLTDIKTSLQNDRLTAHQKLNYHDSNVRFILFLGCMIAMLQQVTGVNV
                     MMYYAPIVLRDVTGSAQEALFQTIWIGVIQLVGSIIGAMIMDKMGRLSLMRKGTIGSI
                     IGLLLTSWALYSQTTGYYALFGMLFFMIFYALSWGVGAWVLISEIFPNRMRSQGMSIS
                     VGFMWIANFMVSQFFPIINENPWLLAHFHGAFPMWIFAACCVFSYFFICRYLPETKGV
                     SLEKMEDIVLTKRRNKPQPIQAERSSD"
BASE COUNT          391 a          343 c          330 g          467 t
ORIGIN      
        1 ggtcaaatag atagatgatt tctgctcagg agataatcag ctatgcccat atcagtgaat
       61 cgttacaata caggctatat attacgtatc tgttctattg cagcattagg cgggatatta
      121 tttggctacg atactgccgt tatttcaggt gcaatcagtt ctctcaccag ttattttcac
      181 ctatctccgg ccgaaaccgg ctgggcggtt tccagcgtcg ttgtaggttg tgtggtggga
      241 tcattcagct caggttactt atccaaacgt tttggtcgca agaaatcact catgatttcc
      301 gccctgctgt tcacaatctc agcagtggga acatctcttt cctacacctt cctgcacttt
      361 attatttatc gtatcgtagg tggacttgct gtcggcctcg cagcaacagt atctccaatg
      421 tatatgtcgg aggtttcacc taaggacatg cgtggcaggg cattgagtat gcaacagttt
      481 gccatcgttt ttggtcagat cctcatattt tacgtcaact ataaaatcgc ctcgattgcg
      541 accaatacat ggctgattga gattggctgg cgttatatgt ttgctgccgg cattgcccca
      601 tgcattctgt tctgtatttt agtattcttt ataccggaat ccccccgctg gatgatgatg
      661 gctggtcgtg aagaagaaac gctaaaaatt cttacaaaga tatctaacga ggaacatgca
      721 cgacatttgc taacagatat aaaaacctct ctgcaaaatg accggctgac ggcccaccaa
      781 aagctcaatt accatgacag taacgtacgt tttatccttt tcctcggttg catgatcgca
      841 atgttacagc aagtaaccgg cgttaacgtt atgatgtatt acgcacctat tgtattgaga
      901 gatgttaccg gtagcgccca ggaagcgctg tttcagacca tctggattgg cgtaattcaa
      961 ctcgtcggat caattattgg cgcaatgatc atggacaaaa tggggcgttt atcgctaatg
     1021 cgaaaaggca ccattggtag catcattggt ttattattaa cttcatgggc tctgtacagc
     1081 caaacaaccg gctattatgc tctctttggt atgctgttct ttatgatttt ctatgcactt
     1141 tcctggggcg ttggagcatg ggtgctcata tcagaaatat tccccaaccg catgcgttca
     1201 cagggaatga gtatttctgt aggatttatg tggatagcaa actttatggt ttcacagttc
     1261 tttcctataa ttaacgaaaa tccctggtta ctggcccatt tccacggtgc atttccaatg
     1321 tggatattcg cagcctgctg tgtctttagc tatttcttta tttgccgcta cctgccggaa
     1381 accaaaggtg tctcactaga gaaaatggag gatatcgtgc tgacaaaacg caggaacaag
     1441 ccacaaccta ttcaggctga acgctcttca gattaagctt agccaaaatg ccgatgatca
     1501 tgaaaaacag attatcggca tcttcctctt a
//
LOCUS       DAZKFU020000504         1531 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZKFU020000504 DAZKFU020000000
VERSION     DAZKFU020000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.506"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..591
                     /locus_tag="ACHOQQ_RS25070"
                     /old_locus_tag="pgaptmp_005014"
     CDS             <1..591
                     /locus_tag="ACHOQQ_RS25070"
                     /old_locus_tag="pgaptmp_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HGU4473131.1"
                     /translation="VRWPLAELCDYYNDAVRAVILARPDAGASLETLSCVPGARQTLP
                     DGAIQLLDVICLSDGSAIKPVSREVLDAQYPDWHMLRGKPECFISNDLSPRVFWLFPV
                     PDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNPLVDWMLFRAFSKDAAGGAES
                     GLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            588..>1531
                     /locus_tag="ACHOQQ_RS25075"
                     /old_locus_tag="pgaptmp_005015"
     CDS             588..>1531
                     /locus_tag="ACHOQQ_RS25075"
                     /old_locus_tag="pgaptmp_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HGU4473132.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGDT"
BASE COUNT          368 a          350 c          519 g          294 t
ORIGIN      
        1 gtgcgctggc ctctggctga gctgtgtgat tattacaatg atgctgtgcg ggcggtgatt
       61 ctggcaaggc cggatgcagg cgcaagcctg gaaacgctca gttgtgtccc tggcgcacgc
      121 cagactttac ctgatggcgc aatacagctt cttgacgtga tatgcctcag cgatggcagt
      181 gcgattaaac cagtatcccg ggaggtgctt gatgcacagt atcccgactg gcacatgctg
      241 aggggaaaac cggaatgctt tatcagcaac gacctgtccc cgcgcgtatt ctggctgttt
      301 cctgtccctg acaaagagat aagtattgat gcagtggtaa gccggatacc ggaggcagtg
      361 tatgttctga cgcaggacga tgatacgcca gttccactgg aagaggctta tgttaaccca
      421 ctggtggact ggatgttgtt tcgtgctttc agtaaggatg ctgccggtgg tgcagaatcg
      481 gggctggctg cgcagcatta tcagagtttt gttgagcaac ttgggatcaa acagggggca
      541 gacagtgcat tgtctgcccg taaaaaagtg tttaacggag gtggagtgtg agtgttgttg
      601 tttcggggac gctgaaatct cctgatggtg aggcgatatc aggagcaaat attaccctga
      661 cggcgctgac agtttcaccg gatgcgctca gcggcaccag tgcgtcggca gtgacccgtg
      721 aaggtggata ttacggaatg acgatggatc cgggggagta tgcggtttcg gtgacggtga
      781 aagggaagac tgctgtctac ggacgtgtgc gtattgaggg gaccgaaagt acggtgacgc
      841 tcaatatgct gttacgccgc agtcttgttg aggtgagcat acccggagag ctgctgacag
      901 atttccggca gatacagaat aatgtggctg atgaccttgc cactattcgt cgcctgaatg
      961 aagacacggc gacaaaaaac actcaggcca cacagtcaaa agaaagtgca gcagccagtg
     1021 cgaagagtgc atctgacagt gcaaagacgg caaccagcag ggcggctgaa gccggacaaa
     1081 aagcgactga tgccactgag gccgcgaccc gtgcaatcac agcagcgggg aatgcagagg
     1141 aaagctcgac ccgtgccgga gagtctgaaa aagccgccgg agctgatgca gaaaaagcca
     1201 gacagcatgc tgaaaaggcc aggctggcgc aggagagcgc cggagagatc cttaagcggg
     1261 cagaggctgc cactgtcagt gctgaagagg ccagacgtat ggctgagaat gcacgggggc
     1321 cccgggggcc tcagggagaa actggtccga agggggatgt cggtcctaaa ggcgaaacag
     1381 gtccagtggg ccctcaaggg cccgcagggc cgaaaggtga gcgtggtgac gttggtgctc
     1441 agggggctgt agggcccgct ggtccgcgtg gtgagaaggg cgaacagggg gagcgaggac
     1501 cgcagggaat accaggcctg aagggggata c
//
LOCUS       DAZKFU020000505         1527 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZKFU020000505 DAZKFU020000000
VERSION     DAZKFU020000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.507"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..417
                     /locus_tag="ACHOQQ_RS25080"
                     /old_locus_tag="pgaptmp_005016"
     CDS             <1..417
                     /locus_tag="ACHOQQ_RS25080"
                     /old_locus_tag="pgaptmp_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473133.1"
                     /translation="RYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLPRVARLADLGE
                     NNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPDSNTLGLRNALAIAGA
                     KNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            427..699
                     /locus_tag="ACHOQQ_RS25085"
                     /old_locus_tag="pgaptmp_005017"
     CDS             427..699
                     /locus_tag="ACHOQQ_RS25085"
                     /old_locus_tag="pgaptmp_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HGU4473134.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGATIGATVGSAVPVVGTAVGAVAGAVIGGIAGALF"
     gene            710..>1527
                     /locus_tag="ACHOQQ_RS25090"
                     /old_locus_tag="pgaptmp_005018"
     CDS             710..>1527
                     /locus_tag="ACHOQQ_RS25090"
                     /old_locus_tag="pgaptmp_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473135.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMIAHSQPLMEAIGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIA"
BASE COUNT          421 a          353 c          446 g          307 t
ORIGIN      
        1 agatacggtt ataccggagc agcccgccct cagggggact ggcagacatc cgcagcgctg
       61 acccgccagc aatacgacga ctggcgcacc agatatttac cccgtgtagc aaggctggct
      121 gaccttgggg agaacaacag tctgatgaat gcacagcttg cacgggtggg aggccttgcc
      181 acttccagtc tccgtacagc gcagatggcg caggataacc agatggcgag atacggggta
      241 aaccgcccgg ataatcccga cagtaatacg ctggggttac gtaatgccct ggcaattgct
      301 ggcgcgaaaa atggtatccg tgaagccgaa caggatcgcc agatgaatat tctgacgggg
      361 gcttctgcac cggcaagaca gaaactgagt gttggcggac aactggtggc agcgtaaggg
      421 ggcaatatgg gatacggttt actggatatt gcaaatcagt cgcggcgtga ggcattacag
      481 ggaataagtg acgcagacag acgacgtaca gagattgagg catcaaataa acaactggct
      541 gctcaacaga aagctcagaa aaaacagaat atcggtacgg gcattggtac gggggccact
      601 atcggggcga ctgttggttc agcagtaccg gtagtgggga cagccgtcgg cgctgttgct
      661 ggtgcagtaa ttggcggcat tgcaggcgct ttgttttaag gagtggtgaa tgagcggatt
      721 tgcacagggg ttacttgctg gattcagcac tgttgatcag gcaatgaccc gtcgtaagga
      781 gcttggtttg cgagaagcac agcttgctca gcaacagaaa aataacgagc gcgattttga
      841 atttgcgcag tctcagtttg aacataataa agacgttgat cagcggaact ttgattacag
      901 agccaaagtt gatgaccgca attacacact gcaggaaagg gagtttaacg ccaaccagaa
      961 ttaccggaat gcgtcgctgg ggatggaaca gcaacgactc cagttgcaga aatacaacca
     1021 gcgacggctt gagtataacg atatgattgc ccatagccag ccactaatgg aagcgattgg
     1081 aaaagcaatt gaggctggcg atcaggaggc tgcaacgcgt ctgttcgggc agctgccaaa
     1141 gggacatcca ttaattctta tgtcaaacga aggctatgca gcgaaagcgg gtcaggccgt
     1201 gatcaacctg caaaaaatct ttggtgataa gccggacatg gcgatcgatt cgctgaatac
     1261 cccggaaaat ctcgatgtgc tttccggcgt gtttgccccg gaactacaac agcgtattgg
     1321 catgcctgat tcaaccgggg aaaaaacgat aaaagaggcc aggattggca gtatcgtacc
     1381 ggcgcagcag gaagggtacg tacttattgg ccttgatctc acatacagcg atggctccac
     1441 cgcgcataaa cctgtaacag aatacggcag tgcgcaccct gatgatcaaa ccgtgctggc
     1501 gatacccgtt gataaggcta tcgctca
//
LOCUS       DAZKFU020000506         1527 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZKFU020000506 DAZKFU020000000
VERSION     DAZKFU020000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.508"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..1479
                     /gene="narK"
                     /locus_tag="ACHOQQ_RS25095"
                     /old_locus_tag="pgaptmp_005019"
     CDS             88..1479
                     /gene="narK"
                     /locus_tag="ACHOQQ_RS25095"
                     /old_locus_tag="pgaptmp_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGU4473136.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT          273 a          370 c          427 g          457 t
ORIGIN      
        1 agttatctta tcgtttgatt tacatcaaat tgcctttagc tacagacact aaggtggcag
       61 acatcgaaac gagtatcaga ggtgtctatg agtcactcat ccgcccccga aagggctact
      121 ggagctgtca ttacagattg gcgaccggaa gatcctgcgt tctggcaaca acgcggtcaa
      181 cgtattgcca gccgcaacct gtggatttcc gttccctgtc tgctgctggc gttttgcgta
      241 tggatgttgt tcagcgctgt tgcggtgaac ttaccgaaag tcggttttaa ttttacgacc
      301 gatcagctat ttatgttgac tgcgctgcct tcggtttctg gcgcgttatt acgtgttcca
      361 tactccttta tggttcctat cttcggtggt cgtcgctgga cggcgttcag caccggtatt
      421 ctgattattc cttgtgtctg gctgggtttt gccgtgcagg atacctccac gccttatagc
      481 gtcttcatca tcatctctct gctgtgcggc tttgctggcg cgaacttcgc ttccagtatg
      541 gcaaacatca gcttcttctt tccgaaacag aagcagggtg gcgcgctggg cctgaatggt
      601 ggtctgggca acatgggcgt tagcgttatg cagttggttg ctccgctggt ggtatcactg
      661 tcgattttcg cagtatttgg tagccagggc gtcaaacagc cggatgggac tgagctgtat
      721 ctggcgaatg cgtcctggat atgggtgccg ttccttgcca tcttcaccat tgcggcgtgg
      781 tttggcatga acgatcttgc tacctcgaaa gcctccatca aggagcagtt gccggtactc
      841 aaacggggtc atctgtggat tatgagcctg ctgtatctgg caaccttcgg ctcctttatc
      901 ggcttctccg cgggctttgc gatgctgtca aaaacgcagt tcccggatgt tcagattctg
      961 caatacgctt tcttcgggcc gtttattggt gcgctggcgc gttctgcagg tggtgcatta
     1021 tctgaccgtc tgggcggaac tcgtgtcacg ctggtgaact ttattctgat ggcgattttc
     1081 agcggcctgc tgttcctgac cttaccgact gacgggcagg gcggaagctt catggcgttc
     1141 ttcgcggtct tcctggcgct gttcctgaca gctgggctgg gtagtggttc cactttccag
     1201 atgatttcag tgatcttccg taaactgaca atggatcgcg tgaaagcaga agggggttct
     1261 gacgaacgtg cgatgcgtga agcggcaacc gacacggcgg cggcgctggg tttcatctct
     1321 gcgattggcg cgattggtgg cttctttatc ccgaaagcgt ttggtagctc gctggcatta
     1381 acgggttcgc cagtcggcgc aatgaaggta tttttgattt tctatatcgc ctgcgtagtg
     1441 attacctggg cggtatatgg tcggcattct aaaaaataaa tcttaatatt tgattatcct
     1501 ttgcgcggca tgatgtcgcg ctttttt
//
LOCUS       DAZKFU020000507         1507 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZKFU020000507 DAZKFU020000000
VERSION     DAZKFU020000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.509"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..417
                     /locus_tag="ACHOQQ_RS25100"
                     /old_locus_tag="pgaptmp_005020"
     CDS             <1..417
                     /locus_tag="ACHOQQ_RS25100"
                     /old_locus_tag="pgaptmp_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473137.1"
                     /translation="RYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLPRVARLADLGE
                     NNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLGLRNALAIAGA
                     KNGIREAEQERQMNILTGASAPARQQLSVGGQMMTA"
     gene            428..679
                     /locus_tag="ACHOQQ_RS25105"
                     /old_locus_tag="pgaptmp_005021"
     CDS             428..679
                     /locus_tag="ACHOQQ_RS25105"
                     /old_locus_tag="pgaptmp_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HGU4473138.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            690..>1507
                     /locus_tag="ACHOQQ_RS25110"
                     /old_locus_tag="pgaptmp_005022"
     CDS             690..>1507
                     /locus_tag="ACHOQQ_RS25110"
                     /old_locus_tag="pgaptmp_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473139.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHRKENDQRTYDLNVRNADREYALKEREHKAAQNYRNASLGLSQQRLQLEKY
                     NQRRLEYNDMLARDQSVMVALGKAIDAGDHDAASRLFGKLSDANPIKIISTEGYAAKA
                     GQAVNNLQKIFDDKPDRAIDSLNTPENLDVFSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQNVLAIPVDKAIAL"
BASE COUNT          406 a          339 c          452 g          310 t
ORIGIN      
        1 agatacggtt ataccggagc tgcccgccct cagggggact ggcagacatc cgcagcgctg
       61 acccgccagc aatacgacga ctggcgcacc agatatttgc ctcgagtagc aaggctggct
      121 gaccttgggg agaacaacag tctgatgaat gcacagcttg cacgggtggg aggccttgcc
      181 acttccagtc tccgtacagc gcagatggcg caggataacc agatggcgag atacggggta
      241 agccgcccgg ataatcccga cagtaatacg ctggggttac gtaatgccct ggcaattgct
      301 ggcgcgaaaa atggtatccg tgaagccgaa caggagcgcc agatgaacat actgacgggg
      361 gcttctgcac ctgcaagaca acaactgagt gttggcggtc aaatgatgac agcgtaaggg
      421 ggaagatatg gggtacggct tactggatat tgcgaatcag tcacggcgtg aggcattaca
      481 gggaataagt gacgcagacc gacgacgtga agaaattgag gctgcgaaca aacagatggc
      541 ggcgcaacag aaagcgcaga acaagcagaa tatcggtacg ggcattggta cgggggcggc
      601 tattggcgca tccgttggtg gtcctgttgg tgctgttgct ggtgcagtaa ttggcggcat
      661 tgctggttct ttgttttaag gagtggtgaa tgagcggatt tgcacagggg ttacttgccg
      721 gattcagcac tgttgaccag gcaatgaccc gtcgtaagga gcttggtttg cgagaagcac
      781 agcttgctca gcaacagaaa aataacgagc gcgattttga atttgcgcag tctcagtttg
      841 agcaccgaaa agagaatgat cagcgtacat atgatcttaa tgtcaggaat gctgaccgtg
      901 aatatgcgct gaaagagagg gaacacaaag cagcgcagaa ttatcgaaat gcgtcacttg
      961 ggctatctca gcagagactg caactggaaa aatacaacca gcgacggctt gagtataacg
     1021 atatgctggc acgcgatcag tcggtgatgg tggcgctggg gaaagcgatt gatgcaggtg
     1081 accatgatgc ggcctctcgg ctgttcggca aattatcgga tgccaacccc attaaaataa
     1141 tatcaacgga aggctatgct gcgaaggcgg gtcaggccgt gaataacctg cagaaaatct
     1201 ttgatgataa gccggacagg gcgatcgatt cgttgaatac cccggaaaat ctcgatgtgt
     1261 tttccggcgt gtttgccccg gaactacaac agcgtattgg catgcctgat tcaaccgggg
     1321 aaaagacaat aaaagaggcc aggattggca gtatcgtacc ggcgcagcag gaagggtacg
     1381 tacttattgg ccttgatctc acatacagcg atggctccac tgcgcataaa cctgtaacag
     1441 aatatggcag tgcgcaccct gatgatcaaa acgtgctggc gatacccgtt gataaggcta
     1501 tcgctct
//
LOCUS       DAZKFU020000508         1502 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZKFU020000508 DAZKFU020000000
VERSION     DAZKFU020000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.510"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..423
                     /locus_tag="ACHOQQ_RS25115"
                     /old_locus_tag="pgaptmp_005023"
     CDS             <1..423
                     /locus_tag="ACHOQQ_RS25115"
                     /old_locus_tag="pgaptmp_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473140.1"
                     /translation="DPGAQGPKGETGATGPAGPRGAQGPKGEKGDPGPRGERGETGPM
                     GPPGASGDDGKSRVMGFRLGNPVTYELQEGDHGWSVNLADGSVITGLSGNTQGGVSRV
                     TTIYYRPLQMTFDGSHWRTVGVGEYVSSGAPGREYFSL"
     gene            465..1136
                     /locus_tag="ACHOQQ_RS25120"
                     /old_locus_tag="pgaptmp_005024"
     CDS             465..1136
                     /locus_tag="ACHOQQ_RS25120"
                     /old_locus_tag="pgaptmp_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4473141.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGIGRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNEMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQA"
     gene            1212..1394
                     /locus_tag="ACHOQQ_RS25125"
                     /old_locus_tag="pgaptmp_005025"
     CDS             1212..1394
                     /locus_tag="ACHOQQ_RS25125"
                     /old_locus_tag="pgaptmp_005025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473142.1"
                     /translation="MTMLVYGVKTATEMLILYVMLILFPEHPVDVNNFQIFGSESLDI
                     SIYATVDVFCGAWQCD"
BASE COUNT          402 a          296 c          497 g          307 t
ORIGIN      
        1 gaccccggag cacagggtcc gaaaggtgaa acgggagcaa caggccccgc tggtccgcgg
       61 ggagcgcagg gaccgaaagg agagaaagga gaccccggtc ccagaggtga gcgtggagaa
      121 accgggccga tggggccgcc gggggcatca ggagatgacg ggaagagtcg tgtcatgggc
      181 ttcaggctgg ggaatccggt aacgtatgag ttgcaggagg gggaccatgg ctggtcagtt
      241 aatctggctg atggttcagt cattacaggg ctttcgggga acactcaggg cggcgtcagc
      301 cgtgttacaa cgatttacta caggccgctg cagatgacgt ttgatggctc tcactggcgc
      361 accgttggtg ttggtgagta tgtgtcgtca ggagcgccgg ggcgtgagta tttttcatta
      421 taaagcgcat catctttcag acatgtaacc gggaggactt acagatgcac ataaagaatt
      481 tcagacaata cacgccggaa aatccggatg tgccgggtgc gatgtacctg aaatcagagg
      541 atggtcagga ctggtatgag tgtcagtcgt tattttcagc agagacgctg aaggtggttt
      601 ataacagtgc cggagtcatt accggcatcg gcagggtggc gtcagttctg tggccagtgg
      661 gccagagtgt ggtggaggtg gcggatacgg aagaaaaccg caaagctgac atctcggggc
      721 gctggggttt tgacggggag aaaatcacgg acctgctgac cgcggagaaa gcgcgcggga
      781 tgaagggcga tgaaattaac gcctggcgta atgagatgga agcggcgaac tacacgtttg
      841 agcacaatgg gcgtaaatgg gactacggga agtcaacgca gacgcgtctt gagccgtcgg
      901 tggcggcagc gaaagcgggg aaactgccgg aggcgttttt ctggacggat gcggaaaata
      961 atgatgtgga ggtgacagca gaagagctta tagcgctgag tgaagcggca gagcaggcga
     1021 tgttcaccaa agggatggaa atccacgttc gccagcgcac catgaagaag gagctggaaa
     1081 agctgaccag tgcggatgag atactggcat acagagttgg ctgggcacag gcgtaataaa
     1141 aaaggggggc tgataaacag cccctgacca aagaaaagta tcagcggaaa catttagaag
     1201 gatctaaata aatgaccatg cttgtatacg gcgtaaagac tgccacggaa atgttaattc
     1261 tgtacgtgat gctgatcttg tttccggagc atcctgttga tgtgaataat tttcagattt
     1321 tcggaagtga gagtttggat atttccatat atgccacagt tgatgtgttt tgtggcgcct
     1381 ggcagtgtga ttaagggaac agggataacg aaaaaagcgg gggtatcacc accccccggt
     1441 acaaggaagt attaactata atggaaacat taatgaagac attcagtgct atgttatatg
     1501 gc
//
LOCUS       DAZKFU020000509         1478 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZKFU020000509 DAZKFU020000000
VERSION     DAZKFU020000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.511"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..180
                     /locus_tag="ACHOQQ_RS25130"
                     /old_locus_tag="pgaptmp_005026"
     CDS             <1..180
                     /locus_tag="ACHOQQ_RS25130"
                     /old_locus_tag="pgaptmp_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU4473143.1"
                     /translation="SYLRLSKNGIEHGSSGEFIVKTSDYLVPGSGVDLPNEAPGFSLT
                     DITQESKISSKSFND"
     gene            196..>1478
                     /locus_tag="ACHOQQ_RS25135"
                     /old_locus_tag="pgaptmp_005027"
     CDS             196..>1478
                     /locus_tag="ACHOQQ_RS25135"
                     /old_locus_tag="pgaptmp_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473144.1"
                     /translation="MSVLPKIVWPIPSSRRGSEFKNQEEILSHLGGESTGLYMIGRSG
                     MWHGGIHITEATTPWCALSGKSPLEAIDFPVPFKGEQAIRCMADGEVIAYRICRDYLT
                     IAWESGPLNFSGSFVLVKHYIQPGEKETSGLHFYTLYMHLAPYSAYEAEAENQWVVQD
                     TLRAYSEMDWLTAKLTSDETSPQIAGYMPEGARVEWDPADSNLNATGSNKRKYGLVTL
                     KGLPEKTSSTLTPGQRYWVVVDNNNVKPAAGAGPGWWRHLLPPAKEVMVFDKTVSLSS
                     PFAIKAGDPIGHMGYYQAPKDGGYEARYQVHIECTSMDDNLEKFLTNPEQVGKNNPLW
                     LKYAPGLALYKKDVFTETFTKDTRVTARTGILPLKQVQTEVDKSTKQEYWQLRPENAY
                     VPKGQVEPQLLSQYDLAKLGFRTEIAEPTSFDYLDG"
BASE COUNT          407 a          331 c          407 g          333 t
ORIGIN      
        1 tcttacctga gactgagtaa gaacgggata gagcacggct catcgggtga gtttattgtg
       61 aagacgtctg actatctggt gcctggttct ggcgtagatc tccccaacga agcacctggt
      121 ttcagcctga cggatatcac acaggaaagc aaaatcagca gcaagtcttt taacgactga
      181 cagggagtat gaatcgtgtc tgtattaccc aaaatcgtct ggccgatccc ctccagccgt
      241 cggggaagtg agttcaaaaa tcaggaagag atcctctctc atttgggggg agaatctacc
      301 gggctgtaca tgataggccg tagtggcatg tggcatggtg ggatccatat caccgaagcc
      361 acgacaccat ggtgtgcgct tagcggtaag tctccacttg aagcgataga ttttccggtt
      421 ccttttaaag gtgagcaggc aatccgatgc atggctgacg gagaagtgat tgcttaccgc
      481 atctgcaggg attatctgac gattgcatgg gaatccggtc cgctgaattt ttccggctca
      541 tttgttctgg tgaaacacta tatccagccg ggtgagaaag aaaccagtgg actgcatttc
      601 tatactctgt atatgcatct tgcgccgtat tcagcttatg aggctgaagc agaaaatcag
      661 tgggtcgttc aggatactct aagagcatat tctgaaatgg actggctgac ggcgaaactg
      721 acttcagatg aaacttctcc acaaattgca gggtacatgc ctgaaggggc cagagtggag
      781 tgggacccgg cagacagcaa tcttaacgct acaggaagta acaagagaaa atacggtctt
      841 gtgacgctaa agggtttacc agaaaaaacc agcagtactc tcactcccgg tcagcgctac
      901 tgggttgttg tggataacaa caatgttaag ccagcggcgg gggccgggcc cggctggtgg
      961 cgacacttgc tgcctcccgc aaaggaagtc atggtattcg ataaaacagt aagcctcagt
     1021 tctccgtttg ccattaaggc cggtgacccg attgggcaca tgggctacta tcaggcacca
     1081 aaggatgggg ggtatgaggc acgatatcag gtacatatcg aatgcacgag tatggatgac
     1141 aatctggaga aattcctgac caatcctgag caggtgggaa aaaataatcc actctggctg
     1201 aagtacgccc cggggcttgc gttgtacaag aaagatgttt ttacagaaac attcacgaag
     1261 gacacgagag tgacggccag gacgggaatt ctgccactga aacaggtgca aacagaagtc
     1321 gataagtcga ccaaacagga atactggcaa ttgcggccag aaaacgcgta cgtgccaaaa
     1381 ggtcaggtgg aacctcaact tttgtcacag tatgatctgg caaagctggg gttcagaact
     1441 gaaatcgcag agcctaccag ctttgattac ctggatgg
//
LOCUS       DAZKFU020000510         1472 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZKFU020000510 DAZKFU020000000
VERSION     DAZKFU020000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.512"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..378
                     /locus_tag="ACHOQQ_RS25140"
                     /old_locus_tag="pgaptmp_005028"
     CDS             <1..378
                     /locus_tag="ACHOQQ_RS25140"
                     /old_locus_tag="pgaptmp_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4473145.1"
                     /translation="VAPIMMEQNHGAIITMSSVVGTDGNIGQSNYAATKGGVIAMSKG
                     WAKEFSRKGAKVRVNCVAPGFVITPMTENLPEKVINHMIAKTPLGRMATPEDISNGVL
                     FLASEKSSFITGQTLKIDGGLVI"
     gene            388..>1472
                     /locus_tag="ACHOQQ_RS25145"
                     /old_locus_tag="pgaptmp_005029"
     CDS             388..>1472
                     /locus_tag="ACHOQQ_RS25145"
                     /old_locus_tag="pgaptmp_005029"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGU4473146.1"
                     /translation="MEKVYITGAKRTPIGCFGGSLSTVSASDLGAIAISAAIKQSRIN
                     VECIDEVIVGNVLSAGQGMGPGRQAAVKAGIPWKTPALTLNMICGSGMKTIIDAAMKI
                     RSGDMDVVVTAGIENMSQAPYLIDHHARSGVRMGNQVMSDSLITDGLTDAFNHYHMGI
                     TAENIARQFNITRYEQDTFALESQKRALKAIDNGEFEDEITPVQVHTRKGNIEVTTDE
                     YPRRDSSLEKLSVLRPAFEKNGTVTAGNSSGINDGAAALILTSEKAIQTLELQPLAEI
                     RSFGQGGVDASVMGLGPVPAIDNALKKAKLDIKDIDLFEINEAFAAQAIGVLKSISEN
                     HSVSIDWLNQRTNVNGGAIALGHPLGAS"
BASE COUNT          473 a          228 c          353 g          418 t
ORIGIN      
        1 gttgcaccta ttatgatgga acaaaatcat ggggcaatca taacaatgtc ttctgttgta
       61 ggaactgatg gtaatatagg tcagagtaat tatgccgcaa ctaaaggtgg tgttattgca
      121 atgtctaaag gttgggctaa agaattttcc cgaaaaggtg ctaaagtacg cgtgaattgt
      181 gtagctccag gattcgttat taccccgatg acagaaaatc tgccagagaa agtcattaac
      241 catatgatag ctaaaacccc tttagggcga atggcaacac ctgaggatat ttcgaatggt
      301 gttctttttt tagctagtga gaaatcatct tttattactg gtcaaacatt aaaaattgat
      361 ggtggattag ttatttaagg aggaggaatg gaaaaagtat atattacagg ggcaaaaaga
      421 acgcctatag gatgttttgg cggttcgtta tcaacagtgt cagcttcaga tcttggagca
      481 attgcgatat ctgcagcaat taaacagagc agaataaacg ttgaatgtat tgatgaagtt
      541 attgtcggga atgttttgtc tgctggacaa ggaatggggc caggtagaca agctgctgtt
      601 aaagccggta taccatggaa aactccggct ctgactttga atatgatttg tggtagtggt
      661 atgaaaacta ttatcgatgc tgcaatgaaa attcgtagtg gagatatgga tgttgttgtt
      721 acggcaggca tagagaatat gagtcaggca ccttacttga ttgatcatca tgcaagaagt
      781 ggtgtacgaa tgggtaatca ggtaatgagt gactctctta ttacagatgg tcttactgat
      841 gcttttaatc actatcatat ggggattaca gcagaaaata tagcccgaca gttcaatatt
      901 acaagatatg aacaggatac atttgcgctt gaaagtcaga agagagcctt aaaggccatt
      961 gacaatggag aatttgagga tgaaattact ccggtacaag tacatacccg aaaaggaaat
     1021 attgaagtta ctacagatga gtatcctcgt agagactcct cattagagaa actttctgtg
     1081 ttacgtccag catttgaaaa aaatggtacc gtgactgcag gaaattcatc tggaataaac
     1141 gatggtgcag cagctcttat tcttacttca gagaaagcaa ttcagacatt ggaattacaa
     1201 cctttggctg aaattcgctc atttggacaa ggaggcgttg atgcatcagt gatggggctg
     1261 gggcctgttc cagcaataga taatgcatta aaaaaagcaa aactggatat taaagatatt
     1321 gatctttttg aaattaacga ggcgtttgcg gctcaggcaa taggggtctt aaaaagtatt
     1381 tctgaaaatc actcagttag tattgattgg ctaaatcaaa gaacaaatgt taatggaggg
     1441 gcgatagctc taggccatcc tttgggagca tc
//
LOCUS       DAZKFU020000511         1465 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZKFU020000511 DAZKFU020000000
VERSION     DAZKFU020000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.513"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1404
                     /locus_tag="ACHOQQ_RS25150"
                     /old_locus_tag="pgaptmp_005030"
     CDS             <1..1404
                     /locus_tag="ACHOQQ_RS25150"
                     /old_locus_tag="pgaptmp_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGU4473147.1"
                     /translation="WSVLYLKQHLRRNISTRNSFSAETYRDEFNRFIKRFPVIGSSTH
                     SIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNVIVVGDRKQLPHVPVLLPNSP
                     SPPAEYYNCEKYSLLDSVCMSFRNMVPVTLLKEHYRCHPKIIQFCNKQFYDNALIPLT
                     LDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDEESSRGYIAPYNAQVNLAETV
                     LPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQQSRNIAFVDNPELVNVAVSRARNK
                     FTLVTGNDVFERHAGHIAALIRYIRYYADDGEIFESPVISAFDLLYSEYDKSLERLNS
                     RLNSDDSRFKSEQIVACLLRDILSQDSYRSMMFHSQIALNQLVLLERGDFTHRELLFM
                     RNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERDELKNSILKKCGLPLLRLRTI
                     DSDIEGKLGAFLSGLSG"
BASE COUNT          408 a          268 c          355 g          434 t
ORIGIN      
        1 tggtctgtgc tttatttaaa acaacatctg cgaagaaata tttcaacaag aaattcattc
       61 tcggcagaga catacaggga tgaatttaac cgttttataa agcgttttcc ggttattggt
      121 agcagcacgc attctattat taactctatc ggtaaggggg ctcttcttga ttatgtgatt
      181 attgatgaag cctcacagca ggatattgtt ccgggcattc tgggactagg atgtgcccgt
      241 aatgtaatcg ttgtagggga tcgaaagcaa cttccacatg ttcctgtatt actcccgaac
      301 agcccatctc caccggcaga atactataat tgtgagaaat atagtctact tgattcggtt
      361 tgtatgtcgt tcaggaatat ggtgccagta actcttctta aagagcatta tcgctgtcat
      421 cctaaaatca ttcaattttg taataagcag ttttatgata atgctctgat accgctaacg
      481 cttgattcag gtgaagcctc attatctctg atcatcacgg caaaaggaaa ccatacccgt
      541 aatttttcaa atctcaggga gcttgaatct ctggaaggac attactggga tgaggaaagt
      601 agcaggggat atatcgcccc atataatgca caggttaatc tggctgaaac agtgctgccc
      661 gctgattttg tgaaatcgac ggtgcataaa tttcagggac gggagtgcga tgaaattgtg
      721 ttttccactg tgctggataa gaaacgcagc agccagcaaa gtcggaatat cgcgtttgtt
      781 gataatcctg aactggtgaa tgtcgctgtt tcaagggccc gaaacaaatt tacgttggtg
      841 actggtaatg atgtatttga acgccacgct ggacatattg cggcactgat tcgttacatc
      901 agatactacg ctgatgacgg agagattttc gagagcccgg tgatatcagc atttgatttg
      961 ctgtacagtg agtatgacaa atcactggaa cgactgaata gtagattgaa ttcagatgat
     1021 tcacgcttta agtcagaaca gattgtggcc tgcctgttgc gggatatcct gtcgcaggat
     1081 agttatcgga gtatgatgtt tcattcacaa atagcgctga atcagttggt gttgctggag
     1141 cggggagatt ttactcaccg tgaactgtta ttcatgcgta atcgggccag ttgtgacttt
     1201 gttgtttact ataaggtcgg gaagactcca ttaggggtta ttgaagtgga tggaggctat
     1261 catctgacca gtgtccaggc tgaacgtgat gaacttaaaa acagtatact gaagaaatgt
     1321 ggtctgccat tgctaagatt acgtactata gacagtgata tagaagggaa gttgggcgcg
     1381 tttttgtcag gcctgtcagg ctaaagtctg aatattaaaa attgcatata gcactattag
     1441 ttttctggta agcgtaaacc gaccg
//
LOCUS       DAZKFU020000512         1447 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZKFU020000512 DAZKFU020000000
VERSION     DAZKFU020000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.514"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            40..1447
                     /locus_tag="ACHOQQ_RS25155"
                     /old_locus_tag="pgaptmp_005031"
                     /pseudo
     CDS             40..1447
                     /locus_tag="ACHOQQ_RS25155"
                     /old_locus_tag="pgaptmp_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
BASE COUNT          390 a          335 c          454 g          268 t
ORIGIN      
        1 ataatagata tttcttggta aggaaaatat tccccgaaag aggccacatg gggaatactg
       61 ctcccctcac tggtacggga gtaccgttac gacagcgcgg gtaacgtatc aggcgtcacc
      121 agccgggaag attacggacg ggaaacacag cgggagtacc ggcttgaccg gaacggccag
      181 gtcacggcgg tgacagcctc aggcaccggg ctgggctatg gcgaaggcga tgagtcctat
      241 ggctatgaca gttgtggcta cctgaaggcg cagtctgcgg gcgggcaccg gataagtgaa
      301 gagactgacc agtatgccgg aggccaccgg ctgaaacagg ccggaaacac gcagtatgac
      361 tatgacgccg caggccggat ggtcagccgc accagacacc gtgacggcta tcgcccggaa
      421 acagagcggt tccggtggga cagccgggac cagctgaccg ggtattgcag cgcacagggt
      481 gagcagtggg aataccgcca cgacgccagc ggcagacgga cggaaaaacg ctgtgaccgg
      541 aagaaaatcc gttttacgta cctgtgggac ggcgacagta ttgcggaaat ccgggaatac
      601 cgcgatgatg aactgtacag cgtacggcac ctggtgttta acggctttga gctgataagc
      661 cagcagtgca gccgggtacg gcagccgcac ccgtccgtgg ccccgcagtg ggtgacacga
      721 acgaatcatg cggtaagcga cctgacgggc cgcccgctga tgctctttaa cagtgaaggc
      781 aagacggtct ggcgaccggg acagaccagc ctgtgggggc tggcactcag cctgcccgca
      841 gacacagact acccggaccc gcgcggggag ctggacgcgg aagccgaccc cggcctgctg
      901 tatgcaggac agtggcagga tgcagaatcg gggttgtgct ataaccggtt ccgatattac
      961 gagccggaaa ccgggatgta cctggtgagt gatccactgg ggttgctggg gggtaagcag
     1021 acttaccggt atgtgccgaa tccactgggg tatattgatc cgctggggct ggtaaaaact
     1081 tctgttcccg cagaaaaaat aagcctttcg gataaagcaa gagatctttt tagacaagga
     1141 aaagtaagag aagcattaga tgttcattat gaggatcttg tacgaagaaa gcttggtggt
     1201 atatctcaag aaattgccgg gcgtgaatat gatgttgtta ctgacaagat catagcgcag
     1261 gtcaagagga cttatagctc tatagataat ccaaaaaact tcttaagtaa gtctactcgg
     1321 acacaaatta agaagacaat tgagctggct gaagaacagg ggaaagaggc tcagttttgg
     1381 tttaaatatg gagtaagccc taaagtaaga gaatatatag aaagcaaagg tgggaaagtt
     1441 atattgg
//
LOCUS       DAZKFU020000513         1446 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZKFU020000513 DAZKFU020000000
VERSION     DAZKFU020000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.515"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(318..734)
                     /locus_tag="ACHOQQ_RS25160"
                     /old_locus_tag="pgaptmp_005032"
     CDS             complement(318..734)
                     /locus_tag="ACHOQQ_RS25160"
                     /old_locus_tag="pgaptmp_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4473148.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCARLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
     gene            complement(734..988)
                     /locus_tag="ACHOQQ_RS25165"
                     /old_locus_tag="pgaptmp_005033"
     CDS             complement(734..988)
                     /locus_tag="ACHOQQ_RS25165"
                     /old_locus_tag="pgaptmp_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HGU4473149.1"
                     /translation="MKITTLSSRELNQDVTRAKRATSYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
BASE COUNT          413 a          316 c          313 g          404 t
ORIGIN      
        1 gccatcgaat gactttatta gggctgcgtt tgggaaacgt acaatatggg gcagctcacg
       61 aaacggatat ttttgtacgc taagagacag ctatcgaagc caaagctgcg ccattcttgt
      121 tgttgctcat actgctgtca ggttggagtg tacttcagtc tgacgtcagg ttgtttgggc
      181 gaaacacgtc agaatagggt ctatttcgaa ttttctggac actcctgacg cggagtctgt
      241 gacttcaacc tgacacctta cgctcttata gtacgattcc gtttcccaaa cggagcccta
      301 gaagaaggga agcgatatca agcccaggga ttaataattg atactccggt taattcaaaa
      361 tcaccaacat ttctggttac aaccgtcatc ccatgtacca gagccgttgc tgcgatcaat
      421 gcgtctcgtt cactgcgcct gtcaggaaca tgtaaacgag cacaacgtaa tgcaaccgca
      481 gtatctacag gaagtatctt atcagaaaac gctggtaaca catgctgatc catccaggaa
      541 cgtagcataa tcccttgtcg catatctcga cgttcaactt gtaatattcc ctgttcaagt
      601 tccattatgg aaataacaga aataaagaaa tctgccgcat ctgtatgcaa tgcccatttt
      661 gcaacattat tatctatacg tcctgtccga gccttccgta gctcagagat aacattagta
      721 tcaagaatat acactataaa aactccgcag gttttaaact aaccgtaata cgaggcggtt
      781 caaattcaac tgcatcagca tcgggcatcg ccagaatgtc aatgagagtt cgtcgttgtt
      841 gcgtcaattt tcggtattct tcgatactaa gaagtacatg tgcggtttta ccccgatcag
      901 taataaatac gggaccataa cttgtggcgc gtttggcccg ggttacatcc tgattcagtt
      961 cccgactgga tagagttgta atcttcatgc tgttgccttt tgattcatat aggtatgtta
     1021 ctacattgtg gcccatgaaa tagtacaatg caagaaatgt acttgattag gggtagtctt
     1081 cgccgcttgt tgctgtcaga tattaatgct aatgtatgca tacattagca ttaatggcca
     1141 cgtcctggat gaggttctac gctggaactt catcggataa tctaaactga aagtctgttg
     1201 gctaaaaaac agtcttaacg tggtttattt tccgaattcc aagcggaccc catttaagac
     1261 ttaatacagt aataatgtgt attattacac attattatcc atcaggaagc acgtcgtcag
     1321 tattggagaa aacttacaaa cgctcgccgc agccccagcg accgaacgtt agtgagcgag
     1381 tgggcgagga agcggaagag tgtcaggtct gaattgagca catacgccag aaattatata
     1441 gtaaaa
//
LOCUS       DAZKFU020000514         1446 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZKFU020000514 DAZKFU020000000
VERSION     DAZKFU020000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.516"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(248..529)
                     /locus_tag="ACHOQQ_RS25170"
                     /old_locus_tag="pgaptmp_005034"
     CDS             complement(248..529)
                     /locus_tag="ACHOQQ_RS25170"
                     /old_locus_tag="pgaptmp_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473150.1"
                     /translation="MKTNKKGTKWHIFYRENSGAEVLLEIPSFRECLSVSKELMTPSN
                     YMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
     gene            complement(587..979)
                     /locus_tag="ACHOQQ_RS25175"
                     /old_locus_tag="pgaptmp_005035"
     CDS             complement(587..979)
                     /locus_tag="ACHOQQ_RS25175"
                     /old_locus_tag="pgaptmp_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473151.1"
                     /translation="MELTVDLLKSLNNIRQNATIYVHAYHYTDDEKCAMVPRISLPSL
                     KLTPLQSEIIIRLLLNGVDYIRHDGYVQGYREKTVATFTIQGEGRHRFKADLSQNGES
                     DIASNVLNHWLHRIHKTDIIVLSVVLTE"
BASE COUNT          375 a          284 c          307 g          480 t
ORIGIN      
        1 ttgaatgtgt ggggtttttg gggcgagtaa tgtacgcgcg tattctctag aggtttaatt
       61 taaaaggcta cgtatgtagc tttatgtatt taagagtact gaactcagtc tccggcaata
      121 aaccagtttg gcgtccagct aacaaactct tgcggatgaa aggtccgggc acagcgatga
      181 cttacaagca ttctcccgca aaaaatgaac tatagcgcct tgcttaatgc attgtctgaa
      241 tccggagtta ctttttaata attctgttaa tggttattag ctcacctaat atttcgaagt
      301 tatcaggtgg gcagttaatc catttgttag agccagcgat gatttccctg tcccagcgtt
      361 ttattctttc gccatttttt tctatgcata tcatgtaatt tgatggggtc attaattctt
      421 tagatactga aagacattcc cgaaatgatg gtatttcaag caaaacttcc gcgcctgaat
      481 tttcacggta aaaaatgtgc cattttgtgc cttttttatt agttttcacg tgcgtatgct
      541 cataggataa atataaaacc ttgtaacatt caggaggtaa caggatttat tctgtaagaa
      601 caacagacag aacaatgata tctgttttat gtatacgatg tagccagtga tttagtacat
      661 tgctggctat atcactttcc ccattttgtg ataagtctgc tttgaaacga tggcgtccct
      721 ccccctgtat tgtgaaagtt gccacagttt tttctctata cccctgaaca tatccgtcgt
      781 gccttatata atcgactcca ttgagaagca gtcgaataat tatttctgat tgcaacggag
      841 ttaatttcag tgatggtaaa gatatccgtg gcaccatagc gcatttttca tcatcagtat
      901 agtggtatgc gtgaacatat atggtggcat tttgcctgat gttgttaagg cttttcagca
      961 ggtctacagt caactccatc gcggtatcct tcaggttgtt atcattcgtt gatgatgcga
     1021 tctgttgaag gcgttggcga caattaattc tttttctctt ctgaaaaatt tacggaatac
     1081 aaaaaagatc tgtttttaac tgattgttac tttctgaaga tattctctgc tccatttgct
     1141 ttccgggtaa gacgtatggc tggttatggt atggtcggac tgcagccaca gggggatgac
     1201 tgtaatacct ctgatcacca cagtcagtca tctgtaaatg ttaaatacct cacagttcag
     1261 tcagtggatt attgtcggtc ttacgggata tttgttaaaa aaatactgca tggtgaatcc
     1321 ccctgagcgg cggggcatat cagtatcgta ggtgtttggt cacatccctt atctgtgcgg
     1381 gttcaggtgc tgatactgaa ctcaccggga ggcacccggc accatgctac ataacactcc
     1441 attacg
//
LOCUS       DAZKFU020000515         1426 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZKFU020000515 DAZKFU020000000
VERSION     DAZKFU020000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.517"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1426
                     /locus_tag="ACHOQQ_RS25180"
                     /old_locus_tag="pgaptmp_005036"
     CDS             <1..>1426
                     /locus_tag="ACHOQQ_RS25180"
                     /old_locus_tag="pgaptmp_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HGU4473152.1"
                     /translation="AKARATEEIDLINKKLKFASKIFNFFVDSNIEDSIPFGYVREKV
                     FSLISKDDMHLVSEFFDKNKIDVIEYQWQYIDSHHLKIANSIRKLFLAIDIVYDNEKS
                     VIGQQITAAKDDLRGKGKLDAKDLRVIHPSEKNYIVKEGEINTQRFEFHMYSRVSNHL
                     ENGVFYAAESEQNKRLEDDLIERSYWHKNKSILIEKTGLERLITPITQTLNELENTFS
                     DKLARITIGINSDANEFVKKQPHSNRLQWSLACKKWKTSIDNPIYNQIKNIGIVDVMK
                     YVNNETNFLSVFNAISSRKNNLEADYNDLLACIFGNGANYGVHRMASASDRSIGILRD
                     VNDKFIRPETTGMANTVISDAIANLSVYKYWTINEDSPFGSVDGQKHTCRINTFKARY
                     SAKYFRKGKGVSAMTLVSNHVPLSTVVISPNEYEGHFAFDLLYNNTSEIQPKSLATDN
                     HGINNVNFAILDIFGYQFSPRYAK"
BASE COUNT          503 a          237 c          281 g          405 t
ORIGIN      
        1 ttgcaaaagc tcgggcaaca gaggaaatag atctaataaa taaaaagctt aagtttgcga
       61 gtaaaatatt taacttcttc gttgatagta acattgaaga cagcatccct tttggatatg
      121 ttcgtgaaaa ggttttttca cttatatcga aagatgatat gcatcttgtg agtgaatttt
      181 ttgacaaaaa taaaatcgat gtaattgaat atcaatggca gtatattgac agccatcacc
      241 ttaagatcgc caattcaatc cgaaagcttt ttctggctat agatatagta tatgacaacg
      301 agaagtctgt tattggccag caaattactg ctgccaagga tgatctcaga ggaaaaggaa
      361 agctggatgc taaggatctg agggtaattc atccatccga gaaaaattac attgtaaaag
      421 aaggagaaat taacactcaa cgattcgaat ttcatatgta cagcagggta tctaaccatc
      481 tggaaaacgg tgtcttttat gctgcggaga gtgaacaaaa caagagactg gaagatgatc
      541 tcatagaacg aagttactgg cataagaata aatcgatttt gatagagaaa acaggccttg
      601 aacgacttat cacaccaata acgcagactc ttaatgagct ggagaatacc tttagcgata
      661 aactggctcg tataaccatt ggaataaact cagatgctaa tgagtttgtg aaaaaacaac
      721 cacactccaa cagattacag tggagtctgg cctgtaaaaa atggaagacc agcatcgata
      781 atccgattta caatcagata aaaaatatcg gaatagttga cgtaatgaag tatgtaaaca
      841 atgagacgaa ttttttgtct gtgtttaatg ccatatcatc caggaaaaat aatcttgaag
      901 ctgactataa tgaccttctt gcctgtatat ttggtaatgg cgcaaattat ggtgttcaca
      961 gaatggcatc tgcatcagac cgtagtattg gaatactacg tgatgtgaat gacaaattca
     1021 ttcgtccaga aacaacagga atggccaaca cagtaatatc tgatgcaatt gcaaatcttt
     1081 ctgtatataa atactggacg ataaatgaag attctccatt tggaagtgtt gatgggcaga
     1141 agcacacttg cagaataaat acatttaaag cccgttattc tgccaagtat ttcagaaagg
     1201 ggaaaggggt ttctgcgatg acactggtat ctaatcatgt gccattatca actgtggtaa
     1261 tttctccaaa tgaatatgaa ggtcattttg catttgactt gttatataac aacaccagtg
     1321 aaatacaacc aaagtcatta gcgactgaca atcatggtat aaacaacgtt aattttgcga
     1381 ttcttgacat ttttggctat cagttttcac cacgttatgc aaaatt
//
LOCUS       DAZKFU020000516         1414 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZKFU020000516 DAZKFU020000000
VERSION     DAZKFU020000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.518"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..190
                     /locus_tag="ACHOQQ_RS25185"
                     /old_locus_tag="pgaptmp_005037"
     CDS             <1..190
                     /locus_tag="ACHOQQ_RS25185"
                     /old_locus_tag="pgaptmp_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4473153.1"
                     /translation="TKINEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSAT
                     YHYLNKHNVFRITLPQRN"
     gene            complement(298..1149)
                     /gene="hchA"
                     /locus_tag="ACHOQQ_RS25190"
                     /old_locus_tag="pgaptmp_005038"
     CDS             complement(298..1149)
                     /gene="hchA"
                     /locus_tag="ACHOQQ_RS25190"
                     /old_locus_tag="pgaptmp_005038"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HGU4473154.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
BASE COUNT          369 a          306 c          355 g          384 t
ORIGIN      
        1 aacgaaaatt aatgagcctg aaaaactctt ccgtagattt tggcggggag ataattcgcg
       61 tcattccgta ggtcagggac ttggtctttc tttagtcaaa gcgattgccg aattgcatgg
      121 gggaagtgct acgtatcact atctcaataa gcataatgtg ttccggatta cgttaccgca
      181 aagaaattaa catagggctt cagtacgcca taaattaacc gtgaaaaaac gcagccacgt
      241 cgtgagtact aacgcggctg cgattgatta tgcgcttaca ttcaaacgta acagggatta
      301 acccgcgtaa gctgccagca tttcctgcgc cgccagttta cccaacgcat tcgctgcaaa
      361 aggactgtcg ccggtgagaa gtttacggtc cttatgtact cgcccggtga tgtcgtcatt
      421 aatgatattc atgcccattt tcttcagttc ttcgccgaag taccaggtga gatgacccgg
      481 catatagcca atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc aaatggaata
      541 accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc cgtggcaaag
      601 ggagataaca aaacggtcat ttttgattgc ccactgtaaa gcggcagcca cgtcctggct
      661 ttcaggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg catattcgct
      721 atcagcgttg aggctggcga cgacatccgc gagtttcttc ggattgcgga acaacgattt
      781 atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc agtattcaaa
      841 cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaacctgcag catggagatg
      901 atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt ttccgttatc
      961 ggtcggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgtttac cgcgatacgg
     1021 ttttggatag tccacgccat caagatcaga gacaggactg gtatattggc taagcgaata
     1081 ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt tacttgtttg
     1141 aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata tttgcgactg
     1201 agctggtcgc tatttttaac ccggctagaa aattgagtca atatatcgaa agcggtagtc
     1261 ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt cacgccaaat
     1321 tttccttatt gagtaacgtc gtcgcaagtg gcctcaatga ttttagacca ttatcagagg
     1381 tacggggtgg cggggagaga tttagtgctg tgga
//
LOCUS       DAZKFU020000517         1400 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZKFU020000517 DAZKFU020000000
VERSION     DAZKFU020000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.519"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1400
                     /locus_tag="ACHOQQ_RS25195"
                     /old_locus_tag="pgaptmp_005039"
     CDS             <1..>1400
                     /locus_tag="ACHOQQ_RS25195"
                     /old_locus_tag="pgaptmp_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4473155.1"
                     /translation="ILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPVRVDITAH
                     PAPDRIQVSALPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKE
                     AIVDNGARFEPLSGSLNSVIPPAVQHLTVEVSASDGQYMALAKWDTPRVVKGVRFSLR
                     LTSGSGENSRLVTSAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTAFRINAP
                     AAPASIELTPGYYQITAVPHLTVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGSQW
                     SVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQG
                     MWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLA
                     ALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVA
                     QGNQLFMNDVFLKRLFA"
BASE COUNT          323 a          380 c          437 g          260 t
ORIGIN      
        1 atcctgtcca tcgatgccgc cagccgcaca ctgacgctgg accgtgaggt gacactgccg
       61 gagacaggta catcgacggt gaacctgatt aacggcagcg gtaagccggt gcgcgtggac
      121 atcactgcac accccgcccc ggaccggata caggtcagcg ccctgccgga tggcgtggag
      181 acatacggtg tgtggggact ctccctgccg tcactgcgtc gtcgcctgtt ccgctgtgtt
      241 tccatccggg aaaacacgga cggcaccttt gccatcacgg cagtgcagca cgtaccggaa
      301 aaagaagcca tcgtggataa cggggcccgc tttgagccgc tctccggttc actgaacagc
      361 gtcatcccgc cggcagtgca gcacctcacg gtggaggtga gtgcctcaga cggccagtat
      421 atggcgctgg caaaatggga cacgccgcgg gtggtgaagg gcgtgcgctt cagtctgcgc
      481 ctgaccagtg gcagtggtga aaacagccgc ctggtgacca gcgccatcac tgccgacacg
      541 gagcaccgtt tcagtggcct gcctctgggg gaatacaccc tgacggtcag ggcgataaac
      601 agctacggcc agcagggcga acctgccacc accgcgttcc ggattaatgc gcctgcagca
      661 ccggccagca ttgagctgac gccggggtat tatcagataa cggcggtccc gcatcttacg
      721 gtgtatgacc cgacggtaca atttgagttc tggttttcgg aggcaaaaat cgcagacaca
      781 tctcaggtgg aaacctctgc ccgttatctg gggacaggca gccagtggag tgtctccggc
      841 ccgcacatta agccggggaa ggatttctgg ttttatgtgc gcagcgtcaa cctggtgggg
      901 aaatctgcgt ttgtggaagc cagtggccgg gccagcaatg atgcggaagg gtatctgggg
      961 ctgtttcggg aaaagatagg aaaactgcat ctggcacagg ggatgtggga gctgattgat
     1021 aacagccagc ttgatgatga gatggcggag atgaagacca ccatcacaga aacccgcaat
     1081 gaaatcacac agacggtcag taaaaccctg gaagaccaga gtgccaccat acagcagata
     1141 cagcgggtgc agacagacac aaataacgac ctggctgcgc tgtacatgct gaaggtgcag
     1201 aaaacaaaaa acggcattcc gtatgttgcc ggtataggtg cggggattga ggatgctgat
     1261 ggccagcccc tgagcaatat tctgctgcag gcggaccgta tcgcgatgat taacccggag
     1321 aacggcaaca ccacgccgct gtttgtggcg caggggaatc agctgttcat gaacgatgtg
     1381 ttcctgaagc ggttgtttgc
//
LOCUS       DAZKFU020000518         1397 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZKFU020000518 DAZKFU020000000
VERSION     DAZKFU020000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.520"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..569)
                     /locus_tag="ACHOQQ_RS25200"
                     /old_locus_tag="pgaptmp_005040"
     CDS             complement(<1..569)
                     /locus_tag="ACHOQQ_RS25200"
                     /old_locus_tag="pgaptmp_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative coupling factor TraD, PFGI-1 class"
                     /protein_id="HGU4473156.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTEHSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHT"
     gene            complement(566..1354)
                     /locus_tag="ACHOQQ_RS25205"
                     /old_locus_tag="pgaptmp_005041"
     CDS             complement(566..1354)
                     /locus_tag="ACHOQQ_RS25205"
                     /old_locus_tag="pgaptmp_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473157.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGKRMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQVFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEAQLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEVLSALPEKALPRSWWRRLLQRGTRS"
BASE COUNT          303 a          450 c          358 g          286 t
ORIGIN      
        1 atggtgtgcc ccacacgttc acccagtgcc agcgtcacat cctgctcatc cggctcaata
       61 ccatgcaggg caggatttcc tcccaccggc ggcaggggac ggacgggatt cagcggcgag
      121 tcacgacgaa gaagctgccc aatccaggga agactgtgct ccgttttcat ttcaagccag
      181 cgcgctgtcc ggtacagact gcccggctgc agatagtgtg caacctccgg tcgcagcgtg
      241 tcctgaaggc gctgggtgtg cttctgtgtc cagcggaagc cgcgtcccag aaacagccgc
      301 tgacggctga ccggaatttg ccgggaactc atcacataac ggggcagccg acgcagattg
      361 cgacggtaac ggatgatctt catcccctgt cggaaacgga ccactgccag aacgccaaag
      421 ccggcggctg tgacatagct gaccgaaggc gcgagcgcca ccgcccaggg cgctgtcaca
      481 cagacaccgg ccgccgttgc ggccaccacg gcggtattca gttccacagc cggacgcagc
      541 agagcctcaa tcacataacg gttactcatg accgggtacc tctctgtaaa agacgccgcc
      601 accatgagcg cggcaacgct ttctccggta atgctgacag tacctccagt ctgcgctgta
      661 acaccatcac ctcccgccat gcgtctgccg cattcagcag taatgcctta tacaccagtg
      721 gcccgtcatg tccgccggca tgcacatcat ccactgtcaa ctgcgcctct gttctgaaac
      781 gatccacttt ccagcaggcc aggtcttcgc gggaaacaca ctgccgccgg atctgcagac
      841 gaagatacac atcatgccgg gacaacggtt ctgccaggtt gtgaatttcc cgacaggcag
      901 accggaacac ctggtctgtg tgataaatac tctgggccac attgtgaagc tgtgtgcgcc
      961 cctcaccctg caggtggaac agaaagcggg ccagacagct gcggtgatta ccgtcagcac
     1021 tgacatacca gtgaatgcca tccgtgctga caaaacccat cccggcatgc tgttgctctg
     1081 cctgcgtgta atatcccgga ttcttttcaa gtaacgggat attgatgtcc atgcgtttac
     1141 cacgctccag cagctccagc cagctcattc cggcatactg tggatggtca gtccccacaa
     1201 tgcggtacac attgatactg ccctgtcccg tccagtagtg ttccgtaata aaatccgaca
     1261 ttgccgcaaa ctctgcggta tcccatgcaa ccagttgttc atgtgcccag gccggagcat
     1321 tcgcctgtat ccgctctgta aatgccccct tcattcagct cttctctcct gcacttttga
     1381 gataccgata ccgtcag
//
LOCUS       DAZKFU020000519         1392 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZKFU020000519 DAZKFU020000000
VERSION     DAZKFU020000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.521"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..69)
                     /locus_tag="ACHOQQ_RS25210"
                     /old_locus_tag="pgaptmp_005042"
                     /pseudo
     CDS             complement(1..69)
                     /locus_tag="ACHOQQ_RS25210"
                     /old_locus_tag="pgaptmp_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="traU family protein"
     gene            complement(104..550)
                     /locus_tag="ACHOQQ_RS25215"
                     /old_locus_tag="pgaptmp_005043"
     CDS             complement(104..550)
                     /locus_tag="ACHOQQ_RS25215"
                     /old_locus_tag="pgaptmp_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473158.1"
                     /translation="MSEGTRVNDFAYVKQALLAVFHRMNTRPLTRLCEKDDIQRGIAD
                     IQWMLHHHYYPSPGQVGFIIFLLRDEKFRVVREDGRQSFLAVEIQSLIDVLKDIRKYL
                     QFVTRYDCDGCIIRLHASAERKYLWIFLECVIVFILCAVLFFLIIC"
BASE COUNT          430 a          337 c          245 g          380 t
ORIGIN      
        1 gtcaggcgga tttttttccg tctcccggcg catcacactg cgcccgaacg gatggcactg
       61 tccgctgtcc gctgtccggg tacatgttca ccaccaggtg tttttaacag ataatcagaa
      121 aaaacagcac agcacacaaa ataaacacaa ttacacactc aaggaaaatc cacaggtatt
      181 tacgttcagc actggcatgc agcctgataa tacaaccatc acagtcatac cgtgtcacaa
      241 actgcaggta tttccgtata tctttcagaa catcaattaa cgactgaatt tcaactgcaa
      301 ggaatgactg acgcccatct tcccgcacta cccggaactt ctcatcccgc aataaaaata
      361 ttataaaacc aacctgtccg ggtgatggat aatagtgatg atgtaacatc cattgaatat
      421 ctgcaattcc acgctgaata tcatcttttt cacatagcct ggtgagcggc cgggtattca
      481 tccgatgaaa caccgccaaa agggcctgtt tgacataagc aaaatcattt actcttgtcc
      541 cttcactcat ttaatatcac ccttaaacat ctgttcatgt atcagtttcg gagtgcaaat
      601 ggtcataata ctgatattct tatcgctgtt gttaacggtc ctgcttcgtt ttcgcttcat
      661 aaataacagt ctctttttca gagttctgag aagaccattc ccggctggct ttattttgtt
      721 tttactgtca tccatcatac atctgatgat acaaaaagat tattcacctt tcagcggaaa
      781 catatactct gtaattatca ggtcagggat atctgtaatt acactatggt tttttctgtt
      841 ctttttctca tcgtccatgc tcagaacgcc tgttacgggt ctttatctta ccccctttat
      901 atccagcctg agaatcgata tacatccaca atatattacc agaccatctg tcaaagtatc
      961 ctcaagaatc aacgcagaca agcttgtcgg aaatattgaa catttcatat tatgctatcc
     1021 gtcttataac acgatcacat tacagtctca catcctgcgc cacggaatcc gaaacagaat
     1081 agctaataac ctgaaaaaaa aaggtatact atataaagaa aaggcaagga agacgccact
     1141 ctgtgaaact attgtcctga atctgaggta tgggggaaaa acccgctaca gactgttctc
     1201 ctgcaggagg catcccaatg gattcagagt acatcgcaat ggcggagcat tcattattca
     1261 gacgcagaat aattaatctc attttttcag gagcagatat agcagaataa aactgaacac
     1321 agaccagcaa agcccgactg gatctttagc ccgcaaagaa aaagcggcaa tcccggaaaa
     1381 ataacagccg ga
//
LOCUS       DAZKFU020000520         1388 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZKFU020000520 DAZKFU020000000
VERSION     DAZKFU020000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.522"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(21..407)
                     /locus_tag="ACHOQQ_RS25220"
                     /old_locus_tag="pgaptmp_005044"
     CDS             complement(21..407)
                     /locus_tag="ACHOQQ_RS25220"
                     /old_locus_tag="pgaptmp_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGU4473159.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGVHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
     gene            complement(404..628)
                     /locus_tag="ACHOQQ_RS25225"
                     /old_locus_tag="pgaptmp_005045"
     CDS             complement(404..628)
                     /locus_tag="ACHOQQ_RS25225"
                     /old_locus_tag="pgaptmp_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4473160.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            694..1049
                     /locus_tag="ACHOQQ_RS25230"
                     /old_locus_tag="pgaptmp_005046"
                     /pseudo
     CDS             694..1049
                     /locus_tag="ACHOQQ_RS25230"
                     /old_locus_tag="pgaptmp_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(1269..1388)
                     /locus_tag="ACHOQQ_RS25235"
                     /old_locus_tag="pgaptmp_005047"
                     /pseudo
     CDS             complement(1269..1388)
                     /locus_tag="ACHOQQ_RS25235"
                     /old_locus_tag="pgaptmp_005047"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
BASE COUNT          409 a          338 c          287 g          354 t
ORIGIN      
        1 tgctgattgg atatctatat ctaatttaga ataatggctt taagatggtc agaaaccatt
       61 ttccagttat attctcctgt tgcagcattc aaagcgataa tttgcatttc atcaaacgaa
      121 atacgtgaac taataatatg tacaccattc cgttttaaaa aaacctgtgc aacaaacaag
      181 gctgttcgct tatttgcatc gttgaaaatg tgaccacgag caatagcaac taaatacacg
      241 gcagccagat ccataatatc tgtcacacct tcataatgat acatgttcaa aacacgatga
      301 atgatcgcgt cagcgcgacc gggttctggc atacccgcca cgccaccatc acgcgaaatc
      361 aagcggtcat gaaactcaat catttcctgt gaactaacga atttcatcgc ttagaaagct
      421 ccgtataggc cgtcccatga ctttgcatga tctcagcaaa ctcagcatca agagccgctt
      481 tacggtatcg ttcaaaagcg tctttatcaa ccagtacagc cgcgccacct ttacggcgct
      541 gaatctcaac aggctgattt tcatcaaccg ccagctcgag caacgaggcc aggttatcgc
      601 gagcatggct atagttacat gtaaacatag tgcttcctct ttgagttgta caattaaatt
      661 gtacaacagt tatcttcaat atgaaatcac cgttaccgct ggcgcatcct gacccaactt
      721 aaccggggag aaagtcgcca tgccgttgcc agagctatct gtcacggtca gaaaggtgag
      781 ataagaaagc gatataccga tggtcaagaa gatcaactgg gcgcactggg gctggtgact
      841 aacgccgtcg tattatggaa cactatttat atgcaggctg ccctggacca tttcagggca
      901 cagggggaaa tcgtgaatga tgaagatatc gcacgcctct caccactttg ccacggacac
      961 atcaatatgc tcggccatat tccttcacgc tggcagaact ggtgacgaaa gggcaaaaag
     1021 aggcatcgga ggaagaaaac attgcttaac gtgagttttc gttccactga gcgccagccc
     1081 cctaattctc tatatccaaa ctgaccccaa taggtgacgg gttatgtgac gaaatctgat
     1141 gtagaaatcg ttgtttcagt gacagtcact caatcggtca tttatgtgac accccacgcc
     1201 gttactggtc gcggaaaaat ccaggtttcc ggcttggaac cccatctcaa gagatccttt
     1261 gatcttttct actgaaccgc gatccccgtc agttcagaag acgaagatga cgcaacggtt
     1321 cctcattgta catcacgaat atagtttgct tgacatcctg acaagaataa tattttaatt
     1381 agaaaaat
//
LOCUS       DAZKFU020000521         1370 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZKFU020000521 DAZKFU020000000
VERSION     DAZKFU020000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.523"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            102..752
                     /gene="csgD"
                     /locus_tag="ACHOQQ_RS25240"
                     /old_locus_tag="pgaptmp_005048"
     CDS             102..752
                     /gene="csgD"
                     /locus_tag="ACHOQQ_RS25240"
                     /old_locus_tag="pgaptmp_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGU4473161.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQEHVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            757..1146
                     /gene="csgE"
                     /locus_tag="ACHOQQ_RS25245"
                     /old_locus_tag="pgaptmp_005049"
     CDS             757..1146
                     /gene="csgE"
                     /locus_tag="ACHOQQ_RS25245"
                     /old_locus_tag="pgaptmp_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGU4473162.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1171..>1370
                     /locus_tag="ACHOQQ_RS25250"
                     /old_locus_tag="pgaptmp_005050"
     CDS             1171..>1370
                     /locus_tag="ACHOQQ_RS25250"
                     /old_locus_tag="pgaptmp_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HGU4473163.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDYGIETPSA"
BASE COUNT          407 a          286 c          293 g          384 t
ORIGIN      
        1 attactacac acagcagtgc aacatctgtc agtacttctg gtgcttctat tttagaggca
       61 gctgtcaggt gtgcgatcaa taaaaaaagc ggggtttcat catgtttaat gaagtccata
      121 gtattcatgg tcatacatta ttgttgatca ctaaaccttc tttgcaggcg acggctctct
      181 tgcagcacct taaacaatcg ctggcaatta caggaaaatt acataatatt caacgttctc
      241 tggacgatat atcttcaggc tctattattc ttctggatat gatggaagcg gataaaaagc
      301 ttatccatta ttggcaggat actttgagca ggaaaaacaa caatatcaaa atattgttgc
      361 taaatacgcc tgaagattac ccgtaccgcg acattgaaaa ctggcctcat atcaacggcg
      421 ttttttatgc catggaggat caagaacatg ttgtcaatgg gttgcaaggc gtcctgcgcg
      481 gcgaatgcta ctttacgcaa aagcttgcca gctacctgat tacgcattcg ggtaactatc
      541 gttataacag cacggaatca gccctcctta ctcatcggga aaaagagatc ctgaataagc
      601 tgcgtatcgg cgcgtctaat aacgagatcg ctcgttcgtt gttcatcagc gaaaatacgg
      661 ttaaaacgca tctttataat cttttcaaga agatagccgt aaaaaaccgg acacaagcgg
      721 tttcctgggc aaacgataac ctcaggcgat aaagccatga aacgttattt acgctggatt
      781 gtggcggcag aatttctgtt cgccgcaggg aatcttcacg ccgttgaggt agaagtcccg
      841 ggattgctaa ctgaccatac tgtttcatct attggccatg atttttaccg agcctttagt
      901 gataaatggg aaagtgacta tacgggtaac ttaacgatta atgaaaggcc cagtgcacga
      961 tggggaagtt ggatcactat aacggtcaat caggacgtta ttttccagac ttttttattt
     1021 ccgttgaaaa gagacttcga gaaaactgtc gtctttgcac tgattcaaac tgaagaagca
     1081 ctaaatcgtc gccagataaa tcaggcgtta ttaagtacgg acgatttggc gcatgatgaa
     1141 ttctaaataa aaaattgttc ggaggctgca atgcgtgtca aacatgcagt agttctactc
     1201 atgcttattt cgccattaag ttgggctgga accatgactt tccagttccg taatccaaac
     1261 tttggtggta acccaaataa tggcgctttt ttattaaata gcgctcaggc ccaaaactct
     1321 tataaagatc cgagctataa cgatgactat ggtattgaaa caccctcagc
//
LOCUS       DAZKFU020000522         1369 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZKFU020000522 DAZKFU020000000
VERSION     DAZKFU020000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.524"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..160)
                     /gene="tnpB"
                     /locus_tag="ACHOQQ_RS25255"
                     /old_locus_tag="pgaptmp_005051"
     CDS             complement(<1..160)
                     /gene="tnpB"
                     /locus_tag="ACHOQQ_RS25255"
                     /old_locus_tag="pgaptmp_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4473164.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKM"
     gene            complement(165..833)
                     /gene="tnpA"
                     /locus_tag="ACHOQQ_RS25260"
                     /old_locus_tag="pgaptmp_005052"
                     /pseudo
     CDS             complement(165..833)
                     /gene="tnpA"
                     /locus_tag="ACHOQQ_RS25260"
                     /old_locus_tag="pgaptmp_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            1171..1312
                     /locus_tag="ACHOQQ_RS25265"
                     /old_locus_tag="pgaptmp_005053"
                     /pseudo
     CDS             1171..1312
                     /locus_tag="ACHOQQ_RS25265"
                     /old_locus_tag="pgaptmp_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          350 a          313 c          344 g          362 t
ORIGIN      
        1 ccattttgcc actgcggccc cggaagacga agatatgccc ggagaacggg tcatctttca
       61 gcgtgttctg caccttcgaa gccaggccgt tgaagccaca acgcatatct gtgatgccag
      121 cgatgatcca gattttggta ccggtcggca gcgttatcat cagatccccc tttcatttcg
      181 cgaatgagca ccctaagaag ttccggagtg aggggggaaa acagttttac cacacctgat
      241 ttaagatgca gctcacactg tggcgagcct acggtagtgt cattctggct ttcatcagac
      301 ttgtttcgcc agaaaggatt tgtcaccggt ttgacggggt caagagtctc agtgattgcc
      361 accggaacgg cctggcattc ctggagatcc tcatcattca gtaagccctc ttcatacagt
      421 cttttccagt taaacaacag attgtcatta atattgtgct ctctggcgat ccgtgcaacg
      481 actgcaccag gctgtaatgc ctgttttacc agcctgattt taaattcacg actgtaggag
      541 gttcgtcgtt cttttcgcca tgtgctttcg ctgatttgag gatcatcagg taattccttc
      601 tttctattgg cataaagtat ggcatcgagt tgggcgactg aaagtgattc aggtaatggc
      661 caagtaatac cggaggcatt aaatcgttga aagagattat gaatggtgga atgactgaga
      721 cctaactgca gggcgatagc ccggacagat aattgctcgt cgaatcttag acgtaatgcg
      781 tctggcaaat aagagcgaaa gccggtgata tcttttgctg tctgagaatt catcgttcgt
      841 gtccatcaat atagatggac gcgattgttg ccagacagga caaccatcac aagacgtcac
      901 aggcagagcg cttacggaat ggccttccaa ccgtgttgac gaactgctgc catggaacgt
      961 agttctcacc gataaataag cgtcaatacg gtgctctgtt gacgcttacc ttttactgcg
     1021 aaaatggaca ttagtatcgg aaacaggaaa gggaggcgaa agacggttta aatgatgcgc
     1081 ttacccgcag ggttgaaata accggggaaa catcggtgac tgattaacaa tgaagtgccc
     1141 ccggagtaat tccgggggcc gtaaccggaa tttattttct ttggctgtgc ttatgaaagt
     1201 gaatgaggag ccctgctgta cggggtgagt ctcatgcttc cagaagcaca tctgttatat
     1261 tctgagcgtt ctgccagaat ggccttcctg ccgtgttgat gaactcctgt agaacgaagt
     1321 acttcgccaa taaataaccg tcaatacggc attcgattaa tgcacttac
//
LOCUS       DAZKFU020000523         1369 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZKFU020000523 DAZKFU020000000
VERSION     DAZKFU020000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.525"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(11..427)
                     /locus_tag="ACHOQQ_RS25270"
                     /old_locus_tag="pgaptmp_005054"
     CDS             complement(11..427)
                     /locus_tag="ACHOQQ_RS25270"
                     /old_locus_tag="pgaptmp_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473165.1"
                     /translation="MKNVKGLSDIQDFFNWMWAVDPRLASCLNDWHDYYRRACKRAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMTLVLEMTTHSV
                     AMKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            complement(496..723)
                     /locus_tag="ACHOQQ_RS25275"
                     /old_locus_tag="pgaptmp_005055"
     CDS             complement(496..723)
                     /locus_tag="ACHOQQ_RS25275"
                     /old_locus_tag="pgaptmp_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HGU4473166.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVSFIMQSNVLHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            complement(654..1037)
                     /locus_tag="ACHOQQ_RS25280"
                     /old_locus_tag="pgaptmp_005056"
                     /pseudo
     CDS             complement(654..1037)
                     /locus_tag="ACHOQQ_RS25280"
                     /old_locus_tag="pgaptmp_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1047..>1369
                     /locus_tag="ACHOQQ_RS25285"
                     /old_locus_tag="pgaptmp_005057"
     CDS             1047..>1369
                     /locus_tag="ACHOQQ_RS25285"
                     /old_locus_tag="pgaptmp_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473167.1"
                     /translation="MWRINITWQLVIVCIRGVFLMSDKLPSDYDILFDLWYSYWLERI
                     TCVFHRRVSAALRISAFLVGVAAFVLCDFVVLVLFSVVLFLLWQCMAASFERRAEKAE
                     WQAVS"
BASE COUNT          280 a          338 c          405 g          346 t
ORIGIN      
        1 ttatggtcgc tcatgcgtcg cactccccgg cagtcgccgc ctgaaacgcc gcgcggtaca
       61 gcacgagaag gcgctggtat tcaccgataa agtcttctgc ggtgtatatg cctttcatgg
      121 ccacgctgtg cgttgtcatt tccagaacaa gcgtcatctc tgacgacgac cagctgatgg
      181 cggtgctgtc ggttccgttg atgtgaaata aaccaaaacc atcaccatca cctttcacgt
      241 ccacgcggta acggtcgtca acggtgaagg agaaatcccc gtatgtggtg acgcgtctgg
      301 cggcccgttt acaggcgcgg cggtaatagt cgtgccagtc gttcaggcag gaagccagcc
      361 ggggatccac tgcccacatc cagttaaaaa aatcctgaat atctgaaagt cccttaacgt
      421 tcttcatgag aacctccggt aacagacgtg cggaagcctc ccgcgcacgg gtgcgggatg
      481 gcttcaggat gttgtttagt ggtggtgttg ctggttgata aggtcctgca gttctgccag
      541 gtcttcggcc agatagctga agactgccgc gtaatacccg gcagcgatac cgtcgttaag
      601 tcggtgtaat acgtttgact gcatgatgaa ggagacagat gcagcccgat ggagtctgcg
      661 gttgatttca gtgcggatgt gaagacgctc agcgcgggcg cgctgttgtt tgcggtttgc
      721 catggtgtgg cctcgtacgt agtaagttct gaaaactcac caacccgagg ttccaagctc
      781 agggtggcga gacgtacagg gttggaacta ccggctacgc acgaacccgg cccgaccgaa
      841 gtcggccccg tacgccccgc cataattctg acgtgaaaaa gacgtggcga tacagtacgc
      901 acaaaaaaac cgctggcgcg gttgtgcgcg tgcatagtcg gcggggttcc aatcccggca
      961 cctgttttat gaggtgcggg aaaactgtaa cccgaccagg cgaacgctgg caagcggttt
     1021 ttgtgttgcg tgctgatgtg gttagaatgt ggcgaattaa tatcacctgg caactggtga
     1081 ttgtctgcat acgcggggtg tttctgatga gtgataagct cccttctgat tacgacattc
     1141 tttttgacct gtggtacagc tactggcttg aacgtattac atgtgtgttt caccgacgcg
     1201 tttctgctgc tctgcgaatc agcgcctttc tggttggtgt tgcggcgttt gtcctgtgtg
     1261 attttgttgt gttggtactc ttcagtgtgg ttctgtttct gctttggcag tgcatggctg
     1321 ccagttttga acgcagggct gaaaaggctg aatggcaggc agtcagtta
//
LOCUS       DAZKFU020000524         1363 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZKFU020000524 DAZKFU020000000
VERSION     DAZKFU020000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.526"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(11..421)
                     /locus_tag="ACHOQQ_RS25290"
                     /old_locus_tag="pgaptmp_005058"
     CDS             complement(11..421)
                     /locus_tag="ACHOQQ_RS25290"
                     /old_locus_tag="pgaptmp_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473168.1"
                     /translation="MKNVKGLSDIQDFFNWMWAVEPRLASRLNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTNHSVAV
                     KGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            complement(490..717)
                     /locus_tag="ACHOQQ_RS25295"
                     /old_locus_tag="pgaptmp_005059"
     CDS             complement(490..717)
                     /locus_tag="ACHOQQ_RS25295"
                     /old_locus_tag="pgaptmp_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HGU4473169.1"
                     /translation="MANRKQQRARAERLHTRTEINRRLWRATRVAQIMHINMLHERTH
                     ALSNRYCAAVFSYLADDLAELQDLINQQHHH"
     gene            1041..>1363
                     /locus_tag="ACHOQQ_RS25300"
                     /old_locus_tag="pgaptmp_005060"
     CDS             1041..>1363
                     /locus_tag="ACHOQQ_RS25300"
                     /old_locus_tag="pgaptmp_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473170.1"
                     /translation="MWRINITWQLVIVCIYGVFLMSDKLPSDYEILFDLWYSYWLERI
                     TCVFHRRVSAVLRISAFLVGVAAFVLCDFVVLVLFSVLLFLLWQCMAASFERRAEKAE
                     WQAVS"
BASE COUNT          267 a          336 c          411 g          349 t
ORIGIN      
        1 ttatggtcgc tcatgcgtcg cactccccgg cagtcgccgc ctgaaacgcc gcgcggtaca
       61 gcacgagaag gcgctggtat tcaccgataa agtcttctgc ggtgtatatg cctttcacgg
      121 ccacgctgtg gtttgtcatt tccagaacaa gcgtcatctc tgacgacgac cagctgatgg
      181 cggtgctgtc ggttccgttg atgtgaaata aaccaaaacc atcacctttc acgtccacgc
      241 ggtaacggtc gtcaacggtg aaggagaaat ccccgtatgt ggtgacgcgt ctggcggcct
      301 gtttacaggc gcggcggtaa tagtcgtgcc agtcgttgag gcgggaagcc agccggggct
      361 ccactgccca catccagtta aaaaaatcct gaatatctga aagtccctta acgttcttca
      421 tgagaacctc cggtcacaga cgtgcggaag cctcccgcgc acgggtgcgg gatggcttca
      481 ggatgttgtt tagtggtggt gttgctggtt gataaggtcc tgcagctctg ccaggtcatc
      541 cgccaggtag ctgaagacag cagcgcagta acggtttgat agcgcgtgcg tgcgctcatg
      601 cagcatgttg atgtgcatga tttgcgcgac gcgtgttgcg cgccagagtc tgcggttgat
      661 ttcagtccgg gtgtgaagac gctcagcgcg ggcgcgctgt tgcttgcggt ttgccatggt
      721 gtggcctctt tgctagtaag ttctgaaaac tcaccaacct gaggcggtaa tctcagggtg
      781 gcgagacgta cagggttacc acaaccggct agcaaagaat ccggcccgac cgaagtcggc
      841 cccgtacgcc ccgccataat tcgtgtgcga aaaagacgtg gcgatacagt acgcacaaaa
      901 aaaccgctgg cgcggctgtg cgctttgcta gtcggcgggg tggtaatccc ggcacccgtt
      961 ttatgaggtg cgggaaaact gtaacccgac caggcgagcg ctggcaagcg gtttttgtgt
     1021 tgcgtgctgg tatggttaga atgtggcgaa ttaatatcac ctggcaactg gtgattgtct
     1081 gcatatacgg ggtgtttctg atgagtgata agctcccttc tgattacgaa attctttttg
     1141 acctgtggta cagctactgg cttgaacgta ttacatgtgt gtttcaccga cgcgtttctg
     1201 ctgttctgcg aatcagcgcc tttctggttg gtgttgcggc gtttgtcctg tgtgattttg
     1261 ttgtgttggt gctcttcagt gtgcttctgt ttctgctctg gcagtgcatg gctgccagtt
     1321 ttgaacgcag ggctgaaaag gctgaatggc aggcagtcag tta
//
LOCUS       DAZKFU020000525         1359 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZKFU020000525 DAZKFU020000000
VERSION     DAZKFU020000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.527"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..967
                     /locus_tag="ACHOQQ_RS25305"
                     /old_locus_tag="pgaptmp_005061"
     CDS             <1..967
                     /locus_tag="ACHOQQ_RS25305"
                     /old_locus_tag="pgaptmp_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4473171.1"
                     /translation="EKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRVKQLARRK
                     TITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIAKKLLPY
                     IPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVARVKVAL
                     EYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVGNSSERV
                     PWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEPEEDADDL
                     SVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            complement(1283..>1359)
                     /locus_tag="ACHOQQ_RS25310"
                     /old_locus_tag="pgaptmp_005062"
     CDS             complement(1283..>1359)
                     /locus_tag="ACHOQQ_RS25310"
                     /old_locus_tag="pgaptmp_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="subtilase cytotoxin subunit B"
                     /protein_id="HGU4473172.1"
                     /translation="SNALVGLSTCATSTECFGPDRKKN"
BASE COUNT          391 a          264 c          341 g          363 t
ORIGIN      
        1 tgaaaaaata gatttctggt atgtgatagc tctcgcagga cagtctaatg gtatggccta
       61 cggtgaaggt attcccctgc cagatacact cgacaaaccc gagtcccgtg taaaacaact
      121 ggctcgtcga aaaacaatca caccaggagg aaaagagtgt aaattcaatg agattattcc
      181 ggcagatcac tgcctgcatg atgttcagga tatgagtgga tatcaccatc cggctgctga
      241 tttgcataaa ggggaatatg gttgtgtcgg acagggattg catatagcta aaaagttact
      301 gccatacata cctgagcaag cagggatatt actggttcct tgttgcagag gaggtgccgc
      361 ctttactgtt ggagctgaag gtatgtatgt acctgataca ggggcaacag ctgatgcaat
      421 gcggtggggc accggaacag cactctatga ggacttagta gcccgggtta aggtagctct
      481 ggaatataac agaaagaata aactgctatc tgtctgctgg atgcaggggg agtttgatct
      541 tatgtcacca gattatgaaa agcatcctga cttgttttat cagatggtaa cttctttcag
      601 gtctgagtta tctgaatact catctcagtg tgttggtaat tcgtcagaaa gagttccatg
      661 gttgtgtggt gatacaacct ggtactggaa ggaatcttat caaaaagaat atgattttat
      721 atatggacat tacagacaac ggactgatga tgaaatacat tttctcagct ttcaggatag
      781 taaccgacat gaattaacta atgagccaga agaagatgcc gatgatttat ctgtcgggta
      841 tctggggtca tcatggagga cagaattaag ctggacaacg tcacagagaa gtacacactt
      901 taattcaatg gccagaaggg gagttattgc tgaatgttat gcgcaaaaaa taagaaatta
      961 tttgtagaca agttcaggat atatctacag ctctgacatg attctctgag agacatgacg
     1021 gagtggtatg gagttgtcgg tatcaacacc aggatagctg gtcgttaccg tttgatatca
     1081 ctggtgctga tactatgtgg acggtagtgt gtcacgaagg cacgcaccag gggacgtgac
     1141 tccctgtaat atcatcgaca gtcccattat ggcatgtctg cacctacaac gtcatttcga
     1201 ggtcggctca gtgttcactc gagtcttata tcactgacgc cgacactcca tccagagtat
     1261 ctctggacgg aaacatcacc tattagttct tttttctgtc ggggccaaaa cattcagtcg
     1321 atgtggcgca ggtcgataac ccaacaaggg cattggacg
//
LOCUS       DAZKFU020000526         1355 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZKFU020000526 DAZKFU020000000
VERSION     DAZKFU020000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.528"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1125
                     /locus_tag="ACHOQQ_RS25315"
                     /old_locus_tag="pgaptmp_005063"
     CDS             <1..1125
                     /locus_tag="ACHOQQ_RS25315"
                     /old_locus_tag="pgaptmp_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HGU4473173.1"
                     /translation="APVWWVVVHKLSKGVTQAAVASIPCRPDLRQLRFEIQRTFIENR
                     ALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFMQGDYGTLQSKM
                     PRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEVSDHYWLRLSAAMK
                     MIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAVARIAGTAGSAV
                     AALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLITLTFVVFAVHFL
                     TFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGTMFLVLPAVWLG
                     ALSWAGVHIGDGISRGLPNGISEAKGAASSAGSIANRGIK"
     gene            complement(1173..>1355)
                     /locus_tag="ACHOQQ_RS25320"
                     /old_locus_tag="pgaptmp_005064"
     CDS             complement(1173..>1355)
                     /locus_tag="ACHOQQ_RS25320"
                     /old_locus_tag="pgaptmp_005064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473174.1"
                     /translation="LDSLLKKTISAVLPASDKVSHQNKNKLRNNEFIKIEHPCSKSES
                     RIDGDYIISGGTEYRL"
BASE COUNT          329 a          256 c          418 g          352 t
ORIGIN      
        1 gccccggtat ggtgggtggt ggttcataaa ttgtcaaaag gtgtcaccca ggcggctgtg
       61 gcttcgattc cgtgccggcc tgatttgcgc cagttgcgtt ttgagatcca gcggacgttt
      121 atagaaaaca gggcacttgc tgatgagctg caggacttta cgaacgactg ttattcactg
      181 gcactgtata tgtggaaaca gcgtgatcag gggcagagta aagataaaaa agtgcttgat
      241 gatatctcct ggatagggag cgccacgttc atgcaggggg attacgggac gctgcagtca
      301 aagatgccgc gggctgcgtt cccgtggaat acagcacgtg acagcggacg accggatacg
      361 gggcggggag gctatccgac atgccgggag tggtggagtg acagtagtgt ggggctgaaa
      421 aaacgggtca tggaggaggt cagtgaccat tactggttac gtctgtcagc tgcaatgaag
      481 atgattggca gcagtcaggt ggattataag gaggaggtga tcaagcgtct ggtgagtccg
      541 gcaaacctga cgctctctca ggggggagag gtgtatcgtg gctatggtgg caatgcagat
      601 atgacgacca tgaatgcggt ggcccggatt gcgggaacgg cgggcagtgc ggtagcagca
      661 ctgggtgcgt ttccggcact ggatgcaatg cggcaggcac tgccgatggt acagtcgata
      721 atactgatgg cgatttacat catgataccg ctgattctgg cgtttgcggc atatgaactg
      781 tcgacgttaa tcacactgac gtttgtggtg tttgcggtac actttctgac gttctggtgg
      841 gagcttgcac ggtggcttga cagctggatg ctgacggcgc tgtacagttc ggatacacat
      901 acccggttca atatgatggg attccagaac acatcggatg atctgataat gaatctggtg
      961 atggggacga tgtttctcgt cttgccggca gtgtggctgg gagcgttgtc ctgggctggt
     1021 gttcatattg gcgatggaat cagtcgagga ttaccgaatg gtatttcgga agcgaaaggt
     1081 gcagcttcga gtgcaggaag tattgctaat cgaggtataa aataggaagt aaaaaagtta
     1141 caggagaatt tacagaaaat tctcctgctt aattacaaac ggtattctgt tcctccacta
     1201 atgatgtagt caccatcgat tctggattca gactttgagc atggatgctc aattttaatg
     1261 aattcattat tacgtaattt gtttttattt tggtgtgaaa ctttgtctga tgcaggtaaa
     1321 acagcagaaa tagtcttttt tagtaatgaa tccaa
//
LOCUS       DAZKFU020000527         1354 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZKFU020000527 DAZKFU020000000
VERSION     DAZKFU020000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.529"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(215..>1354)
                     /gene="ompC"
                     /locus_tag="ACHOQQ_RS25325"
                     /old_locus_tag="pgaptmp_005065"
     CDS             complement(215..>1354)
                     /gene="ompC"
                     /locus_tag="ACHOQQ_RS25325"
                     /old_locus_tag="pgaptmp_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4473175.1"
                     /translation="GAANAAEVYNKDGNKLDLYGKVAGLHYFSDDAGSDGDMSYARIG
                     FKGETQIADQFTGYGQWEFNIGANGPESDKGNTATRLAFAGFGFGQNGTFDYGRNYGV
                     VYDVEAWTDMLPEFGGDTYAGADNFMNGRANSVATYRNNGFFGQVDGLNFALQYQGNN
                     EKSGLFDQEGSGNGNGRKLAKENGDGYGMSTSYDFDFGLSLGAAYSNSDRTDNQVHKG
                     THNTRYGDRFDATAGGETAEAWTVGAKYDANNVYLAAMYAETRNMTGYGDADAIANKT
                     QNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGVNSDNFDSQGNHHYTNKDLVKYVDIGM
                     TYYFNKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVGLVYQF"
BASE COUNT          361 a          304 c          315 g          374 t
ORIGIN      
        1 cataaagaaa tgtatttggg gtgttggctg gaagagtagc tggtagaaga tattgtgcca
       61 ggaggggcag ggtaaggtgg tgtatcgata tgctttggca attgcgtgtg ccgcagaggg
      121 agatgaaatg tcaataaaaa agcctgagtt cgatgaggga cgaactcagg ctagtgttca
      181 gttttacagt aacacgccgc agcgagttgg ctgattagaa ctggtagact aagcccacag
      241 caacgatatc atcggtagcg atgccgtttt ctttgtagaa gtcgtcgtcg ttgtccagca
      301 ggttgatttt gtaatcaaca taggtggaca tgtttttgtt gaagtagtag gtcataccaa
      361 tatcaacata tttaaccaga tctttgttgg tgtagtgatg attaccctgg ctgtcgaagt
      421 tatcactatt tacaccgcct aaatccttac ctttagattg caggtaagcg atggacgggc
      481 gcagaccaaa gtcgaactga tactgagcaa caacttcaaa gttttgggtt ttgttagcga
      541 tggcatcagc atcaccgtaa ccagtcatgt tacgggtttc agcatacata gctgctaagt
      601 aaacgttatt agcgtcgtat ttagcaccaa cggtccatgc ttctgctgtt tcaccgccag
      661 cagttgcatc gaagcgatct ccataacgag tgttatgagt acctttgtga acctgattgt
      721 cggtacggtc agagttagag taagcagcgc ccagacttaa accaaagtca aagtcatagg
      781 aagtggacat accgtaaccg tcgccgttct ctttagcaag tttacgtcca ttaccgttac
      841 ctgaaccttc ttgatcaaat aatccgcttt tctcgttgtt accctgatac tggagtgcaa
      901 agttcagacc atcaacttga ccaaagaaac cattgttacg ataggttgct acgctgttag
      961 cacgaccgtt catgaagttg tcagcgccag cataggtatc tccaccaaat tctggcagca
     1021 tatcggtcca agcttctacg tcatatacga caccgtagtt acgaccatag tcgaaagtac
     1081 cattctgacc aaagccgaaa cctgcaaatg ccagacgcgt tgcggtatta cctttgtcgc
     1141 tttcaggacc gtttgcgcca atgttaaatt cccactgacc ataaccagtg aattggtcag
     1201 cgatctgagt ttcacctttg aaaccgatac gggcatatga catgtcgcca tcgctgccag
     1261 catcatcaga gaagtagtgc aggcccgcta cttttccgta caaatccagt ttattaccat
     1321 ctttattata gacttcagcc gcatttgctg cgcc
//
LOCUS       DAZKFU020000528         1353 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZKFU020000528 DAZKFU020000000
VERSION     DAZKFU020000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.530"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1180)
                     /locus_tag="ACHOQQ_RS25330"
                     /old_locus_tag="pgaptmp_005066"
     CDS             complement(<1..1180)
                     /locus_tag="ACHOQQ_RS25330"
                     /old_locus_tag="pgaptmp_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HGU4473176.1"
                     /translation="MFQRSKYDSAYVYALCCVAALAGLMFGYSTAVITGVILPLQQYY
                     QLTSTETGWAVSSIVIGCIIGALVGGKIADKLGRKPALLIIAVIFIISSLWGAMSESF
                     MVFSISRIVSGFAVGMAGTASTMYMSELAPADIRGKALGIYNISVVSGQVIVFIVNYV
                     IAKGMPSDVLVEEGWKSMLFAQVIPSVTMLVITLFLPESPAWCARNNKGEKRSLKVLA
                     RIYRGVSATDVAAIFDSMRETEARENAAGREFVILKSSPVLRYILLVGCCIAVLQQFT
                     GVNVMNYYAPLVLQNSSTEEVMFQTIFIAVCNVIGSFIGMILFDRYGRIPIMKIGTIG
                     SIIGLLIASYGLYTHDTGYITIFGILFFMLLFAVSWSVGAWVLISEVFPEKIKGFGMG
                     L"
BASE COUNT          466 a          362 c          220 g          305 t
ORIGIN      
        1 ccagccccat gccgaatcct ttaatctttt cgggaaacac ctcagaaatc agtacccatg
       61 ctcctacgct ccagctgaca gcaaacaaca gcataaaaaa taaaatccca aaaatggtga
      121 tatagccagt atcatgggta tacaaaccat atgatgcgat caacaaacca ataattgacc
      181 cgatagtgcc aatcttcatg attggtattc gtccatagcg atcgaacaaa atcatcccga
      241 taaaactacc gatcacatta caaacagcaa taaaaatcgt ctggaacatc acttcctctg
      301 tactgctgtt ttgtagcacc agaggtgcgt agtagttcat cacattgaca ccagtaaact
      361 gttgtaaaac agcgatacaa cagccaacca gcagaatata gcgcaacaca ggagagcttt
      421 tcaggataac gaattccctg cctgcagcat tctcccgggc ctccgtttcc ctcatactat
      481 caaaaatagc ggctacatcg gtagcgctaa ccccccggta gattctggca agcactttca
      541 gagaacgttt ttcccccttg ttattacggg cacaccatgc cggcgattca ggcaaaaata
      601 aagttatcac taacattgta acagaaggta tcacctgagc gaataacatg ctcttccagc
      661 cttcttcgac cagcacatca gagggcatcc ccttagcaat cacataatta acaataaaaa
      721 caatcacctg accagaaact actgatatat tgtaaatacc cagagcttta ccacgaatgt
      781 cagctggtgc cagttcagac atatacatgg tagatgcagt ccccgccatc ccaacagcaa
      841 aaccactgac gatgcgcgaa atcgagaaaa ccataaaaga ttcactcatc gccccccaca
      901 gtgacgagat gataaaaata actgcaataa tcagaagcgc aggcttacgc cccaacttat
      961 cggcaatttt ccccccgacc agcgcaccaa tgatacaacc aatcacaatg ctcgatactg
     1021 cccacccagt ttcagttgaa gtgagctggt aatattgctg taaaggcagt atcacccccg
     1081 ttatcactgc cgtcgaatag ccaaacatca atccagccag tgcagcaacg cagcacaatg
     1141 cgtacacata ggctgaatca tacttgcttc tctgaaacat accccctccc tgcctgttga
     1201 ggaaatgaca tacgaacatt tattccatac ttaaacaaat cagctcaaat attccacaac
     1261 aaagatcaca aaatataaaa atttcataac tgtgaaaaat agtttgtagc acaataatca
     1321 tattgaaaat acagaacaat aaaaaactat tat
//
LOCUS       DAZKFU020000529         1348 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZKFU020000529 DAZKFU020000000
VERSION     DAZKFU020000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.531"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(274..1278)
                     /locus_tag="ACHOQQ_RS25335"
                     /old_locus_tag="pgaptmp_005067"
     CDS             complement(274..1278)
                     /locus_tag="ACHOQQ_RS25335"
                     /old_locus_tag="pgaptmp_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGU4473177.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKVLLRKKFTRTKLIEFLAG
                     CASSTVVMEACAGAHFMARRIADLGHEAKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEAQQAMRALHRVRESLVSDKVKTINQMHAFLLEFGISMPTGVAVVKR
                     LSTVLAENELPPYLAQLLMRLHAHYIYLVGQITELEIALEQELSRDEVGQRLQTIPGI
                     GPITASVLSSQLGDGKQYSCSRDFAASTGLVPRQYSTGGKNTLLGISKRGDKNLRRLL
                     VQCARSFIQRIENQSGRLADWVRALLARKHSSVVCCALANKLARIAWAITTQQTVFER
                     "
BASE COUNT          334 a          346 c          303 g          365 t
ORIGIN      
        1 tctatggtcc ccaccatttt tgcaacactg attttgattg atgttggctt gcctaaatct
       61 atccggcgtc attatgggca tggatgcccg cgcctcgatg agttccgcac ctgatgagcc
      121 caaaaaacca tccggcttca gtgagccatt ttcttgctga ggcttttcat caggccgata
      181 tgccgttcat gtcatcaata atcagtcttc gcaaaaccag attgggaact actttaattc
      241 atgattaacc tgccagccag actggcgcca tcgttacctt tcgaacacag tctgctgtgt
      301 tgtgattgcc cacgctatcc gggctaattt gttggccagt gcgcaacata ctacgctgga
      361 gtgttttctt gccaacaacg ccctgaccca atccgccaac cttcctgact gattctcgat
      421 tctctgaata aaggatcttg cgcactgaac gagtaaccgt cgcagatttt tatctccacg
      481 tttacttatc cctaaaagtg tatttttacc acctgtgctg tactgccggg gtaccagacc
      541 ggttgaagca gcaaagtccc gactgcagct atattgttta ccgtctccca gctgcgatga
      601 cagtacgctg gcggttatcg gaccaatacc gggtattgtc tgaagacgtt gtcctacctc
      661 atcacgactc agttcctgct ccagtgcgat ttcaagctcg gtgatctgtc cgacaagata
      721 aatataatgg gcatgtaagc gcatcagtaa ctgcgccaga tagggaggaa gttcgttctc
      781 tgcaagtact gttgaaagtc gttttaccac tgcgactcct gtcggcatac tgatgccaaa
      841 ttccagcaaa aaagcatgca tctgattgat cgttttgact ttatcactga ccaatgattc
      901 cctgacccgg tgaagcgccc gcatggcctg ctgagcttct gttcgtggtt gcacaaatcg
      961 catggaggga cgagatgccg cttcgcatat ggcttctgca tcgacaaaat cgttcttgtt
     1021 gctcttaaca aaagggcgaa cgaactgcgg agaaatcaat tttgcttcat gacctaaatc
     1081 agcaatccgg cgagccataa aatgagctcc ggcgcaggct tccatcacaa cggtagacga
     1141 ggcacagcct gccagaaact caataagttt ggtacgggta aactttttac gcagcaatac
     1201 cttgcctgac ttatcttggc agtgaatgtg aaaggaatgc ttgccaagat ctataccaat
     1261 aagcgtaatg ttttgcatga tggtcttctc catgaaagaa aaacactcta ctagggtaca
     1321 gctcacaggg tggtggggac catctcat
//
LOCUS       DAZKFU020000530         1343 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZKFU020000530 DAZKFU020000000
VERSION     DAZKFU020000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.532"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..577
                     /locus_tag="ACHOQQ_RS25340"
                     /old_locus_tag="pgaptmp_005068"
     CDS             <1..577
                     /locus_tag="ACHOQQ_RS25340"
                     /old_locus_tag="pgaptmp_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4473178.1"
                     /translation="AQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDI
                     HRFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLA
                     ENGFYRVSPSRAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQ
                     RRTHSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            574..1155
                     /locus_tag="ACHOQQ_RS25345"
                     /old_locus_tag="pgaptmp_005069"
     CDS             574..1155
                     /locus_tag="ACHOQQ_RS25345"
                     /old_locus_tag="pgaptmp_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4473179.1"
                     /translation="MNTAAEAIRALSLQVPGFRGQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          296 a          320 c          435 g          292 t
ORIGIN      
        1 ggcacagatg cagggggaga ttgtggcgct ggtccacagt catcccggtg gactgccctg
       61 gctgagcgag gcggaccggc ggctgcagat aaagagtgcc ctgccctggt ggctggtctg
      121 ccggggtgac attcaccggt tccgctgtgt gccgcacctg accggacggc gctttgaaca
      181 cggtgtgacg gactgttaca ccctgttccg ggatgcttac catctggcgg ggataacgct
      241 gccggatttt gtgcgtgagg atgactggtg gcgcaacggt cagaacctgt acctggacaa
      301 cctggcggaa aacggctttt accgggtgtc tccgtcccgt gcacaggcag gcgatattct
      361 gctgtgctgc tttggcgcat cggtgccgaa tcatgccgcc atttactgtg gcaacggtga
      421 actgcttcac catatacctg aacaactgag taaacgggag aggtattcag agaaatggca
      481 acgacgaacg cattctgtct ggcgtcaccg ccactggtcc gcatctgcct tcacggggat
      541 ttacaacgat ttggtcgccg catcagcctg tatgtgaaca cggcagcgga ggccatccgt
      601 gccctgtcgc tgcaggtgcc gggattccgc ggtcagatga atgaaggctg gtatcagata
      661 cgtattgccg gtgaggatac cgcaccggag gcggtgtacg cccgtcttca cgaacaactg
      721 ggtgagggag cggtcatcca cattgtgccg cgactggccg gagccggggg aaatggtgtt
      781 tttcaggtgg tgctgggggt tgcagcgatt gtcgggtcat ttttcactgg tggtgcaaca
      841 ctggcggcat ggggcgcagc cctgagtgcc ggagggctga ctgccaccac gatgctgttc
      901 tcactgggtg ccagcatgat actgggtggt gtggctcaga tgctggcccc gaaggcaaag
      961 acgccggagt ataaaagtac ggataacggt aaacagaaca cgtatttttc gtcactggac
     1021 aacatgattg cccagggtaa cccgatgccg gtgccttacg gtgaaatgct ggttggttca
     1081 cgacggatat cccaggatat cagcacccgt gatgagggcg gagacgggaa agtggtggtt
     1141 atcgggcggg gataaaaata aaaaaaatcc cgcagagtca gcggagctgc gggaaataac
     1201 gatgaagatt aacgttatgg agttattttt ttgggcagaa aaacattaac gcagcgaaat
     1261 tataagcgcc acatggattt tgcgaaaatg tgaagaaatt cagaaatttt ttgctgcaga
     1321 ggtgcaggga gcttgccggg tat
//
LOCUS       DAZKFU020000531         1340 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZKFU020000531 DAZKFU020000000
VERSION     DAZKFU020000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.533"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..132
                     /locus_tag="ACHOQQ_RS25350"
                     /old_locus_tag="pgaptmp_005070"
     CDS             <1..132
                     /locus_tag="ACHOQQ_RS25350"
                     /old_locus_tag="pgaptmp_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4473180.1"
                     /translation="VEETRVVFMRDGYPHPCMRPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            complement(152..385)
                     /locus_tag="ACHOQQ_RS25355"
                     /old_locus_tag="pgaptmp_005071"
     CDS             complement(152..385)
                     /locus_tag="ACHOQQ_RS25355"
                     /old_locus_tag="pgaptmp_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473181.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(481..1104)
                     /locus_tag="ACHOQQ_RS25360"
                     /old_locus_tag="pgaptmp_005072"
     CDS             complement(481..1104)
                     /locus_tag="ACHOQQ_RS25360"
                     /old_locus_tag="pgaptmp_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4473182.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYWYHLSHRIRRMVGSENTSSPYQQIAREVKAPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTSVEVSDNLTGTTRKIPAYHL"
     gene            complement(1193..1340)
                     /locus_tag="ACHOQQ_RS25365"
                     /old_locus_tag="pgaptmp_005073"
                     /pseudo
     CDS             complement(1193..1340)
                     /locus_tag="ACHOQQ_RS25365"
                     /old_locus_tag="pgaptmp_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247776.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          328 a          377 c          306 g          329 t
ORIGIN      
        1 gttgaggaaa cccgggtggt gtttatgcgg gacggctatc cgcatccctg catgcggccg
       61 atgtacaact tcctgggaaa attcaaaccc gaaccccgga aagaaccccc tgccggcaat
      121 cacactgcct gatggcagca taatccactt attaattctc atccccggag acaagcccag
      181 aaaaccactc gggatgttca ctgtatgccg tgttcagcgc atcccaggaa acgttgatat
      241 aaaagctgtc ctgcaatttg cgggtaacaa tttctgccac ccggttacgg ttatccgcct
      301 gtccggcata acctctctct gccagccact cattcagttc atgttccgga gacagattga
      361 taatatcgta atcggacctt gccattcccg tttccttagc acatgttttc aggcacgggg
      421 ataaagtata aagaatgtcg tactgctgct gccagagcaa tattccccct gatggtcgta
      481 tcagagatgg tatgccggta ttttgcgggt tgttcccgtc aggttatcgc tcacttccac
      541 ggacgttgtc accaccggca ttccggcctt tctcagcctc aaaacatcag ctgcaatacg
      601 ctgactacca gaccagaaca ggccgtccag tgcagtcacc agtaaccccg cctccagcgc
      661 atacttcagc cgttcacggg gcgctttcac ttcccgggca atctgctggt atggcgatga
      721 tgtattttca ctcccaacca tccggcgaat acgatgagac agatgatacc agtatgtatc
      781 cggcacaccg gaaaggctgg ccttcaggct gtacacgcag ccaaagcgtt tatcgttgaa
      841 caccacattt ttctggctga tatcccattc ttcacgcagc gcggcaatca gttgtggtgt
      901 acgggtaagc aacaagcgaa aaggcagttc aaaactggtg acgtaatcca cattcagcag
      961 ggcaatgcgc agtcgttctt ctggtccggc ttctgtctgc cggcactcct ccaggacatc
     1021 ctgccactgc aggcgaagac gggaagactc attcagttct gtaaagcagt atttatccgc
     1081 cagataatca attcgtgtat gcatactgaa gagtattccg tataaagatt cagctggcaa
     1141 aactttatca gtctgtaaaa actaacggaa gagtcgatat ttctcccgtc catcaccgga
     1201 tgattgttgc aataccccgt ggcatcagag actgaacagc cgtttttaac gcaacgtatt
     1261 gctctgtcag cgccagcgct tatcaccggc caggtctgtt gcagtaaatc cgccacttgc
     1321 gaacatgctt catcaactgt
//
LOCUS       DAZKFU020000532         1340 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZKFU020000532 DAZKFU020000000
VERSION     DAZKFU020000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.534"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..359
                     /locus_tag="ACHOQQ_RS25370"
                     /old_locus_tag="pgaptmp_005074"
     CDS             <1..359
                     /locus_tag="ACHOQQ_RS25370"
                     /old_locus_tag="pgaptmp_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4473183.1"
                     /translation="SLKHHNCLYASSLVGGNCWHFYIRDEITTLEIAKTVNIENSEFL
                     KAVALMGGGIAYLPYPLVQSEILSGKLVSIMNDYIKTRFEVSLWFRKPLSLRCVTFRN
                     FLVERASQLKDTVQFR"
     gene            533..1340
                     /locus_tag="ACHOQQ_RS25375"
                     /old_locus_tag="pgaptmp_005075"
                     /pseudo
     CDS             533..1340
                     /locus_tag="ACHOQQ_RS25375"
                     /old_locus_tag="pgaptmp_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          400 a          287 c          321 g          332 t
ORIGIN      
        1 tcagtttaaa gcatcataac tgtttatacg catcttctct tgttggaggg aattgttggc
       61 atttctatat tcgtgatgaa ataacaacat tggaaatagc taaaacagta aacattgaaa
      121 atagtgagtt tttaaaagca gttgcactaa tgggtggggg gatagcatac ttaccatatc
      181 ctcttgttca atcagaaata ctgtcaggaa aactagtatc aataatgaat gattatatta
      241 aaactcgttt tgaagtaagt ctgtggttta ggaagcccct atccttgcgt tgtgttacat
      301 ttaggaattt cctagtagaa agagcctcac aactcaagga tactgtacaa ttcagataaa
      361 tcacttaact tgatgcatca atcacccagc gaccagttat gctcatcata atcattattc
      421 aggtgtgagc ataatgagca taacccagcg atccagacag gttccctgaa tctccagaca
      481 accaatatca attaaataag tgatagtcta aaaactagtc attagagaac agatgattga
      541 tgtcttaggg ccggagaaac gcggacggcg taccacacag gaaaagatcg ctattgttca
      601 gcagaacttt gaaccgggaa taactgtctc cctcgttgca cggcaacacg gtgtgacagc
      661 cagttatttc tctggcgtaa gcaataccag gaaggaaatc tcactgctgt ggctgctgga
      721 gaacaggttg ttctggcctc tgagcttgct gccgccatga agcacattaa agaactccag
      781 cgcctgcccg gcaagaaaac gatggaaaat taactcatta gagaagccgt tgaatattgg
      841 cgtgcaaaaa agtggatagc gcacgcccaa tattgcccgg gaatggggag taagtttcgt
      901 cagccgttat ctccgtgtgt tgcgtgcgca gttgcacgtc attctcagac gaaccgatga
      961 ctggaaggat ggccgccgca tgatacggat gtttatctcc gtaataacat ggtgtacacc
     1021 atgttatcgg agagctgccc acgtatggtt atcgtcggct gtgaaacagt acaggacgtc
     1081 atgctgggag cggtagaaca ccgcttcggc agcgaccttc ctgcgtctcc agtggagtgc
     1141 ctgacggata atagttcatg ctatcaggct aatgaaacac gccagttcgc ccggatgttg
     1201 gaacttgaac cgaagaacac ggcggtgcgg tatccggaga gtaacggcat agcagagagc
     1261 ttcgtgaaaa cgataaagcg tgactacatc agtatcatgc ccaaaccaga cgggttaacg
     1321 gcagcaaaga accttgcaga
//
LOCUS       DAZKFU020000533         1338 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZKFU020000533 DAZKFU020000000
VERSION     DAZKFU020000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.535"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..>1338
                     /gene="bluF"
                     /locus_tag="ACHOQQ_RS25380"
                     /old_locus_tag="pgaptmp_005076"
     CDS             151..>1338
                     /gene="bluF"
                     /locus_tag="ACHOQQ_RS25380"
                     /old_locus_tag="pgaptmp_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HGU4473184.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANGLVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPS"
BASE COUNT          370 a          290 c          290 g          388 t
ORIGIN      
        1 ctatacaaga tttttcgcca actcgtgttc aactttttcc attttctatc aatgtaaaga
       61 aacagttatg ttttggtatt aaaagaaaga caccggaaga tatgtctgtt accgtcttac
      121 tctcgcctct cccattaccc tggacagtta atgcttacca cccttattta tcgtagccat
      181 atacgtgacg acgaacctgt caaaaaaatc gaagaaatgg tttcgatagc aaatcgcagg
      241 aacatgcagt ctgacgtaac agggatctta ctgtttaatg gttctcattt tttccagctt
      301 ctggaaggtc cggaagaaca ggttaaaatg atatatcggg ctatatgcca ggatccacgg
      361 cactataata ttgttgagct gctgtgcgat tacgcgcctg ctcgccgttt tggcaaagcg
      421 ggaatggaat tatttgattt gcgcctgcac gagcgagatg acgttttaca ggccgtattc
      481 gacaaaggca catcaaaatt tcagctaact tatgatgaca gagcgctaca attttttcgt
      541 acttttgtcc ttgcaaccga acaatcaacc tatttcgaga tccctgccga agactcctgg
      601 ctttttatcg ctgacggatc tgataaagaa cttgattcct gtaccctttc accaactata
      661 aacgaccact ttgcctttca tcctattgtc gatcccttat cgcggcggat aatcgctttt
      721 gaagccattg tgcaaaaaaa tgaagacagc ccgtcagcta tagcggttgg tcagcgtaaa
      781 gacggggaaa tctacacagc ggatcttaaa agtaaggcgc ttgcattcgc gatggcacac
      841 gcacttgagc tcggtgataa aatgatatca atcaatctat tacctatgac cctggttaac
      901 gagcctgacg cagtctcttt tttacttgat gaaataaagg ccaatggtct ggtgcctgaa
      961 caaatcatcg ttgaatttac tgaaagtgaa gtcatatctc ggtttgatga gtttgccgaa
     1021 gcggttaaat cgctaaaggc tgccggtatc agtgtagcaa ttgatcattt tggcgcaggt
     1081 tttgctggtt tgttactcct gtcacgcttc cagcctgaca gaattaaaat cagtcaggaa
     1141 ttgattacca atgttcataa aagcggacca cggcaggcaa ttattcaggc gatcatgaaa
     1201 tgctgtacat cacttgaaat tcaagtctct gctatgggcg tggcaacacc agaagagtgg
     1261 atgtggcttg aatccgcagg aattgagatg tttcaggggg atctgtttgc taaagctaaa
     1321 ttgaatggta tcccttcc
//
LOCUS       DAZKFU020000534         1336 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZKFU020000534 DAZKFU020000000
VERSION     DAZKFU020000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.536"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1336
                     /locus_tag="ACHOQQ_RS25385"
                     /old_locus_tag="pgaptmp_005077"
     CDS             <1..>1336
                     /locus_tag="ACHOQQ_RS25385"
                     /old_locus_tag="pgaptmp_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473185.1"
                     /translation="SFSLGDVLPTMGKGVAQSVRGTGEMARGLGDAMIQSPVKTGARI
                     LNEFSRMGLPGVATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDG
                     AEATDEWLTGKMSPGAVRALNTPMTEGYDDSAVWLAKGVNLIGALVPDMVAGGVARKV
                     GDVTLRKMLTAGLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQ
                     SAMAAADAVLNADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRA
                     QLATDPELLAVNAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSR
                     YQENTALRETAGMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEETAMR
                     DAETVQQDDAAPQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAA"
BASE COUNT          317 a          314 c          442 g          263 t
ORIGIN      
        1 catcgttcag cctcggtgat gtattgccaa caatgggtaa aggtgttgcc cagtccgtcc
       61 ggggaacagg ggaaatggcc cgtggactcg gtgatgcgat gattcaaagc ccggtaaaaa
      121 caggggcgcg tattttaaat gagttcagcc gtatggggct gccgggtgtc gcaactgtgc
      181 aggatatttt tgccggtggc agcagggggg ctgatgaggt catcgatacc ctgcctgatg
      241 gcaaaaacgc ggttactgat actgtcggta aaggtctgaa ggcaaccggt aaggctgtca
      301 gtgatggtgc tgaagccact gatgaatggc tgaccggtaa gatgtcgccg ggtgcagttc
      361 gtgcgctgaa tacgccgatg accgaaggct atgatgattc tgcggtctgg ctggcgaagg
      421 gtgtaaacct gattggtgcg cttgtacctg atatggttgc tggcggtgtg gctagaaagg
      481 tgggtgatgt cacactgcga aaaatgctga ccgccgggct ggagaaaaaa tacatcgcgg
      541 cagggatgca gccggaaaga gccacggcac tggcagcaga agctgtcgat aaaaaaatgc
      601 cggatttatt ccaggcgggc ctgatcaccc attccacagc cagtgcacag gggcagagtg
      661 caatggcggc agcagatgct gttcttaatg cggattactc tgagctggcg cagtcaccga
      721 aatttcagca gacgtttttg tccattgacg ccgacccgca gcacgcacag cttactgatc
      781 gccagaaaat ggatctggca aaagagcgtg ttgccgatga ggtgcgcgcg cagctggcaa
      841 ccgatcctga attgctggct gtgaatgcca tggcggcaaa actgggtgac gcacaactgt
      901 ttaatctggt gacacgaggc acagcgaaga ccgttaaaag cggcattgtc agaaatgcca
      961 cggaacaggg ggcgattaat gcggcgcagg gcggctattc acgctatcag gaaaacacgg
     1021 cattgcgtga gaccgccgga atggatgtgt caccgtggga gggcgtggct gacgcaacga
     1081 tcgaaggtgc agcctttggt gctgcgatgg gggctccatc cggtgcggtt gccggatatc
     1141 gtggcagacg tcaggccgca gaagaaaccg ccatgcgtga tgctgaaacc gtgcagcagg
     1201 acgacgcagc cccgcaacca gaatctgttg atccggtggc gcagcagcgt gaatccatgc
     1261 agggcatgaa tcgcgagcag cttctggagc agtatgctga tgcggatatg gcaacagagg
     1321 gtgacgcatc cgcagc
//
LOCUS       DAZKFU020000535         1334 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZKFU020000535 DAZKFU020000000
VERSION     DAZKFU020000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.537"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..1093
                     /locus_tag="ACHOQQ_RS25390"
                     /old_locus_tag="pgaptmp_005078"
     CDS             74..1093
                     /locus_tag="ACHOQQ_RS25390"
                     /old_locus_tag="pgaptmp_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HGU4473186.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          381 a          303 c          370 g          280 t
ORIGIN      
        1 gcctcgcaga tttccggcat catagtgccc aatacgggat gcacaaagct ccctcagact
       61 ggagactcca caaatgaaat atacaccggt tggcgttgat atcgcaaaac atgtcattca
      121 gattcacttc atcaatgagc acacaggtga agtggttgat aaacagttgc gtagacagga
      181 ttttctgacg ttcttcggca accgtgagcc atgcctgatt ggtatggagg cctgtggagg
      241 ttctcagcac tgggcacggg aactgacaaa acttggtcat aaagtccggt tgttgcaggc
      301 ccgcttcgtt aaggcattcg tcatgggcaa taagaatgat gtgatggatg ccagggctat
      361 ctggatggcg gttcagcagc cgggtaaaga aatcgccgta aaaacagaag aacagcagtc
      421 ggtactggtt ctgcaccgta cccgcatgca actggtgaag ttccggaccg cacaaattaa
      481 tgccctgcac gggacgttac tggagtttgg tgaaaccatc cacaaaggcc gggcagcgat
      541 ggagcgggag ttccccgaag cactggaacg gatgaaagag agactgccac cgtatctcat
      601 tatggttctg gaaaaccagt acaaccgact gaatgagctg gactcactga tagaggatat
      661 tgaaaaacag cttaccagcg tggcgaggca gaatgaaacc tgtaagcggt tgctggatat
      721 tcctggcgtt ggaccactta ttgcgacggc agcggtggcc accatggggg aagcatcagc
      781 gtttaaatcg gggcgagagt tcgccgcata tgttggtctg gttccaaaac aaactggctc
      841 cggagggaaa gtacgtctgc tggggataag caaacgtggt gacacttatc tcaggacatt
      901 atttatccac ggtgcaagag cggtggcatt agtagctaaa gagcctggcc cgtggataac
      961 cgaactgaaa aaacgtcgtc cagccagtgt ggcaatcgtc gccatggcaa acaagctggc
     1021 acgaacagta tgggcgataa ccgcccatga ccgtaagtat gacaggaacc acgtcagtat
     1081 cagaccatat taatcgctga taccgttaaa caatgaactc ttaacaaaag ggtgaatgct
     1141 gaaaggttgc tatggcggcc agagtgatga caaagacagg taagaccgtg actcactaaa
     1201 cctgaacagt attttgggct tgaagtccgc cgtgaaaata aggggtgagt cggcgaatta
     1261 cataggggct cgcagcgtta cggctgcaat aaagccggat ataaagctgc aacctacccg
     1321 tcatgtcaaa acaa
//
LOCUS       DAZKFU020000536         1313 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZKFU020000536 DAZKFU020000000
VERSION     DAZKFU020000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.538"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(232..903)
                     /locus_tag="ACHOQQ_RS25395"
                     /old_locus_tag="pgaptmp_005079"
     CDS             complement(232..903)
                     /locus_tag="ACHOQQ_RS25395"
                     /old_locus_tag="pgaptmp_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4473187.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVNQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
     gene            complement(945..>1313)
                     /locus_tag="ACHOQQ_RS25400"
                     /old_locus_tag="pgaptmp_005080"
     CDS             complement(945..>1313)
                     /locus_tag="ACHOQQ_RS25400"
                     /old_locus_tag="pgaptmp_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473188.1"
                     /translation="PAGPQGPKGEKGDPGPRGERGETGPMGPPGASGDDGKSRVMGFR
                     LGNPVTYPLQEGENGWSVDLADGSVITGLSGNTQGGVSRVTTIYYRPLQMTFDGSHWR
                     TVGVGEYVSSGASGREYFPL"
BASE COUNT          275 a          432 c          268 g          338 t
ORIGIN      
        1 gcgccataaa acacatcaat tgtggctggt atggaaacac caaaaactct cacttccgga
       61 aatctgaaaa attatccata tcaacaggat gctctggaaa tgagacctgc atcactttca
      121 ggattaactt ttccgtgaca ctctttatct gtgtaggtaa ggtctcttca caataattac
      181 ataatcgact cagttcatgg gggaggtgat ggtcccccat ctttttgtct gttattcctg
      241 tgcccagcca accctgtatg ccagtatctc atccgcactg gtcagctttt ccagctcttt
      301 cttcatggtg cgctgccgga cgtggatttc catccctttg gtgaacatcg cctgctctgc
      361 cgcttcactc agcgcaataa gctcttctgc tgtcaccggc acatcattgt tttccgcatc
      421 cgtccagaaa aacgcctccg gcagtttccc cgctttcgct gccgccaccg acggctcaag
      481 acgcgtctgc gttgacttcc cgtagtccca tttccgccca ttgtgctcaa acgtgtagtt
      541 cgccgcttcc atcgcattac gccaggcgtt aatttcatcg cccttcatcc ctcgcgcttt
      601 ctccgcggtc agcaggtccg tgattttctc cccgtcaaaa ccccagcgcc ccgagatgtc
      661 agctttgcgg ttttcttccg tatccgccac ctccaccaca ctctggttca caggccacag
      721 aactgacgcc accctgctga tgccggtaat gaccccggca ctgttataaa ccaccttcag
      781 cgtctctgct gaaaataacg actgacactc ataccagtcc tgaccatcct ctgatttcag
      841 gtacatcgca cccggcacat ccggattttc cggcgtgtat tgtctgaaat tctttatgtg
      901 catctgtaag tcctcccggt tacatgtctg aaagatgatg cgctttataa tggaaaatac
      961 tcacgccccg aagctcctga cgacacatac tcaccaacac caacggtgcg ccagtgagag
     1021 ccatcaaacg tcatctgcaa cggcctgtag taaatcgttg taacacggct gacgccgccc
     1081 tgagtgttcc ccgaaagccc tgtaatgact gaaccatctg ccagatcaac tgaccagcca
     1141 ttttcaccct cctgcaacgg atacgttacc ggattcccca gcctgaagcc catgacacga
     1201 ctcttcccgt catctcctga tgcccccggc ggccccatcg gcccggtttc tccacgctca
     1261 cctctgggac cggggtcgcc tttctcccct ttcggtccct gcggaccagc ggg
//
LOCUS       DAZKFU020000537         1310 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZKFU020000537 DAZKFU020000000
VERSION     DAZKFU020000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.539"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..631
                     /gene="evgA"
                     /locus_tag="ACHOQQ_RS25405"
                     /old_locus_tag="pgaptmp_005081"
     CDS             17..631
                     /gene="evgA"
                     /locus_tag="ACHOQQ_RS25405"
                     /old_locus_tag="pgaptmp_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGU4473189.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            636..>1310
                     /locus_tag="ACHOQQ_RS25410"
                     /old_locus_tag="pgaptmp_005082"
     CDS             636..>1310
                     /locus_tag="ACHOQQ_RS25410"
                     /old_locus_tag="pgaptmp_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4473190.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAEQNDYFIGSNIITSS
                     MISRYFT"
BASE COUNT          412 a          278 c          251 g          369 t
ORIGIN      
        1 gcaaagggaa taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc
       61 aattcgtaat ttattgatca aaaacgatat tgaaatctta gcagagttga ctgaaggcgg
      121 aagtgccgtt cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat
      181 ccccggagtt aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat
      241 tattattatc gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg
      301 cgctaatggt ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc
      361 tgcaaaaaat ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac
      421 gtccgaccag caaaaactcg actccttatc gaaacaagaa attagtgtca tgcgctatat
      481 tcttgatggc aaggataata atgacattgc tgaaaaaatg ttcatcagca acaaaactgt
      541 cagcacttat aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta
      601 cacattcgca caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc
      661 ttctctgttg tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc
      721 gtggcatcag tagtaacaac cgtgtcacac ttgatccact acgtctgagc aacaaggaat
      781 tacgttggtt agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta
      841 cgttgttgca taccgattcg cagcaacggg ttcgtggtat taatgctgat tatttaaatc
      901 ttttaaaaag agcgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag
      961 caatggacgc gctggaagaa ggtgaagtcg atatagtgtt atcacattta gttgcttcgc
     1021 cgcctcttaa tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg
     1081 tcaccaccct tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc
     1141 gagtagcaaa ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta
     1201 tctcttttac aaatttatat caggcattag catccgtctc agccgaacag aatgattact
     1261 ttattggtag taacatcatt accagcagta tgatttcccg ctatttcact
//
LOCUS       DAZKFU020000538         1304 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZKFU020000538 DAZKFU020000000
VERSION     DAZKFU020000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.540"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1304
                     /locus_tag="ACHOQQ_RS25415"
                     /old_locus_tag="pgaptmp_005083"
     CDS             <1..>1304
                     /locus_tag="ACHOQQ_RS25415"
                     /old_locus_tag="pgaptmp_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HGU4473191.1"
                     /translation="RFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQEL
                     TERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAP
                     ENAAGRQGETLEGEMVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVR
                     DSLPELAQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFG
                     EEIKGAAKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIA
                     LYRSDIPAKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQ
                     LLRTLPPSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDE
                     LLTKAKVWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSG"
BASE COUNT          350 a          329 c          394 g          231 t
ORIGIN      
        1 cgattccgtg gtttcacggg ggatgatacg gaggtacagg cccgccttgc ccgtggcaac
       61 gtgccgacgg cagaggagct tgtacgttca cagatggctg aaggtgatgc cggtccgaca
      121 gcacaggagt taactgagcg tccacgcctg cccgctcccg gcgatattca tcccggacag
      181 ggatatccgt taccgggaga ggtggcgcgt acgccggatg agaatcaggc aggacgtggt
      241 ggtcgtttta ccacaaccgg tgaggttaag ggccagagtt tccagaaagg acaagctccg
      301 gcaccggaaa acgccgctgg tcgccagggg gaaacactcg agggtgaaat ggttcgtcgt
      361 ggtctgccgt caccggatgc gcagaacgcg acagcaccgg tacgtgaagg gctaccggct
      421 cctgacattg cgcgtaatgt tcgtatgcct cagcctgaat cacttccccg cactgtacgg
      481 gactcactgc ctgagcttgc acagcaggca gaagtacgcc gacaggccgg aggaaatcgt
      541 gacatcccgc agcctgagac aatcgcacct gaatctgaaa caactgtctc tactgacagg
      601 gaagctaccg tgcgcggagg tgaagtcagg ggcaaaaaaa ttgaagactt tggcgaggaa
      661 attaaagggg cagccaaaca ccgttatgca cagcttgctg aaacactggg taaaacgctg
      721 gaagacaggg attatgccac gcagccgctg agcaaactgt tcccgaaacc ggactacgca
      781 aaactggcga acgaaggtgc cgatgctgat accctggcaa tgatagcgct gtatcgtagc
      841 gatattccgg cgaagacgaa acacaatacg gcaggctggg gggagagcat aaaaaaagta
      901 cgacacagtg tatcggaaat gctgaacgga acggtcagcg cgaaacgcct cgcagaatgg
      961 atggaaggca gaatgccctc ccgttacgcg gacacctggc aactgttacg cactctgcca
     1021 ccctcacaga tggacagggc ttctgcttat cgggtggtat cgggtgtgta tcaggcggca
     1081 ggagggaagc gttacgatcc gccacagaaa ctttattcac tgcgcaataa ggacaataag
     1141 ggtagtaacc tctttttctc ggaaagcagg gatgaattac tgacaaaggc gaaagtctgg
     1201 tttgcagagc aggaggaaaa atcacaggcg aaaggtgatg aaaaaacagc accgtcaccg
     1261 gatgacaaaa tccgctttga cgtttaccgg aatacccgca gtgg
//
LOCUS       DAZKFU020000539         1304 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZKFU020000539 DAZKFU020000000
VERSION     DAZKFU020000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.541"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1304
                     /locus_tag="ACHOQQ_RS25420"
                     /old_locus_tag="pgaptmp_005084"
     CDS             <1..>1304
                     /locus_tag="ACHOQQ_RS25420"
                     /old_locus_tag="pgaptmp_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HGU4473192.1"
                     /translation="RFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQEL
                     TERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAP
                     ENAAGRQGKILEGETVRRGLPSPDAQNVTAPVREGLPAPDIARNVRMPQPESLPRTVR
                     DSLPELAQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFG
                     EEIKGAAKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIA
                     LYRSDIPAKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQ
                     LLRTLPPSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDE
                     LLTKAKVWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSG"
BASE COUNT          350 a          326 c          394 g          234 t
ORIGIN      
        1 cgattccgtg gtttcacggg ggatgatacg gaggtacagg cccgccttgc ccgtggcaat
       61 gcgccgacgg cggaggagct tgtacgttca cagatggctg aaggtgatgc cggtccgaca
      121 gcacaggagt taactgagcg tccacgcctg cccgctcccg gcgatattca tcccggacag
      181 ggatatccgt taccgggaga ggtggcgcgt acgccggatg aaaatcaggc cggacgtggt
      241 ggtcgtttta ccacaaccgg tgaggttaag ggccagagtt tccagaaagg acaagctccg
      301 gcaccggaaa acgccgctgg tcgccagggg aaaatactgg agggcgaaac agttcgtcgt
      361 ggtctgccgt caccggatgc gcagaacgtg acagctccgg tacgtgaagg gttaccggct
      421 cctgacattg cgcgtaatgt tcgtatgcct cagcctgaat cacttccccg cactgtacgg
      481 gactcactgc ctgagctcgc acagcaggca gaagtacgcc gacaggccgg aggaaatcgt
      541 gacatcccgc agcctgagac aatcgcacct gaatctgaaa caactgtctc tactgacagg
      601 gaagctaccg tgcgcggagg tgaagtcagg ggcaaaaaaa ttgaagactt tggcgaggaa
      661 attaaagggg cagccaaaca ccgttatgca cagcttgccg aaacactggg taaaacgttg
      721 gaagacaggg attatgccac gcagccgctg agcaaactgt tcccgaaacc ggactacgca
      781 aaactggcga acgaaggtgc tgatgctgat accctggcaa tgatagcgct gtatcgtagc
      841 gatattccgg cgaagacgaa acacaatacg gcaggctggg gggagagcat aaaaaaagta
      901 cgacacagtg tatcggaaat gctgaacgga acggtcagcg cgaaacgcct cgcagaatgg
      961 atggaaggta gaatgccctc ccgttacgcg gatacctggc aactgttacg cactctgcca
     1021 ccctcacaga tggacagggc ttctgcttat cgggtggtat cgggtgtgta tcaggcggca
     1081 ggagggaagc gttacgatcc gccacagaaa ctttattcac tgcgcaataa ggacaataag
     1141 gggagtaacc tctttttctc ggaaagcagg gatgaattac tgacaaaggc gaaagtctgg
     1201 tttgcagagc aggaggaaaa atcacaggcg aaaggtgatg aaaaaacagc accgtcaccg
     1261 gatgacaaaa tccgctttga cgtttaccgg aatacccgca gtgg
//
LOCUS       DAZKFU020000540         1302 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZKFU020000540 DAZKFU020000000
VERSION     DAZKFU020000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.542"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..>1302
                     /locus_tag="ACHOQQ_RS25425"
                     /old_locus_tag="pgaptmp_005085"
     CDS             3..>1302
                     /locus_tag="ACHOQQ_RS25425"
                     /old_locus_tag="pgaptmp_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGU4473193.1"
                     /translation="MHDNDCVILGGFIGAVVPECIEKGIEDRFLKTGKPRNLKIIFAA
                     GQGDAGTKAVNHLSHEGLVTSAIGGHWGLIPKLQKLANEGKITGYNLPQGVICHLFRD
                     CAAGKLGTLTHVGLGTFVDPRVEGGKINDKTQEDIVSVTEINGVENLFYKKIDANIAI
                     LRGTTSDQSGNITMEDECLILENLAAAQLVHNNGGKVIVQVKQIVRDGTLDPLQVRIP
                     GIYVDAVVVSEDEYHMQTFAEYMNFSYVGKGVKEVKDKETRPLDIKKIIARRAAMELK
                     KNAVVNYGIGAPEVIAEVTDEEGITSELIATVEPGAIGGTPAGGLSFGASSFPQAIIT
                     QDQMFDFYDGGGLDQAFLGLAETDEKGDLNVSKFGTKIAGCGGFINITQNAKKVYFCG
                     SFTAGKNKIYVSNGKLHIKEDGSVKKFIKKVQQITFSSDTA"
BASE COUNT          424 a          190 c          312 g          376 t
ORIGIN      
        1 tgattcatga taatgattgt gttattcttg gcggatttat tggtgcagtt gttcctgagt
       61 gtatagaaaa aggcatagaa gatcgttttt tgaaaacggg aaaaccaaga aatctcaaga
      121 ttatttttgc tgctggtcaa ggggatgcag gaacaaaagc tgtcaatcat ttatctcatg
      181 aggggcttgt cactagtgcc attggcgggc attggggact aatacctaaa ttgcagaagt
      241 tagctaatga gggaaaaatt acgggttata atcttcctca aggggttatt tgccatttat
      301 tccgagattg tgctgctggg aagttaggga cattaactca tgttggttta ggtacatttg
      361 ttgatccccg cgtagagggc gggaaaatta atgataaaac acaagaggat attgtgtccg
      421 tcacagaaat taatggtgtg gaaaacctat tttataagaa gattgatgct aacatagcaa
      481 tcctcagagg tactacatct gatcagagtg ggaatataac aatggaggat gaatgtttga
      541 ttcttgaaaa cctagctgca gcccagttag tccataataa tggagggaaa gttattgttc
      601 aggtaaaaca aatagtacgt gacggtacat tagatccttt acaagtcaga attccaggta
      661 tttatgttga tgctgttgtt gtttctgaag atgaatatca tatgcaaact tttgcggagt
      721 atatgaactt cagctatgtc gggaaaggtg taaaagaggt aaaagacaaa gaaacacgcc
      781 ctcttgatat aaaaaaaatt atagctcgac gagccgcaat ggaactgaaa aaaaatgcag
      841 ttgtaaatta tggcataggg gcacctgaag ttatagctga agtaacagat gaagagggga
      901 ttacctccga acttatcgca actgttgaac caggtgcaat tggaggaacg cctgctggag
      961 gtctgtcatt tggagcttct tcgttccctc aggcgataat tactcaggat cagatgtttg
     1021 atttctatga cggtggtggg cttgatcaag cgtttcttgg attagctgaa acagatgaaa
     1081 aaggagattt aaatgtttct aaatttggaa caaaaattgc agggtgcgga ggatttatta
     1141 acataactca aaatgctaaa aaagtatatt tctgcggttc tttcactgct ggaaaaaata
     1201 agatttatgt cagcaatggg aaattgcata taaaagagga tgggagtgta aaaaaattca
     1261 ttaagaaggt tcaacaaatc actttctcgt cagatactgc aa
//
LOCUS       DAZKFU020000541         1295 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZKFU020000541 DAZKFU020000000
VERSION     DAZKFU020000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.543"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1289
                     /locus_tag="ACHOQQ_RS25430"
                     /old_locus_tag="pgaptmp_005086"
     CDS             <1..1289
                     /locus_tag="ACHOQQ_RS25430"
                     /old_locus_tag="pgaptmp_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4473194.1"
                     /translation="SYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPGINPYINFD
                     WPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKINACIHNTG
                     AEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYNVRSIVLDG
                     VVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQFHELLSKL
                     HHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNEIETALSAP
                     IASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSDSPDFCELW
                     PIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTINGDGHTLA
                     LTGVNLCVDKAVVHHLITPQKPENIYCPGNSEAEIQ"
BASE COUNT          418 a          288 c          264 g          325 t
ORIGIN      
        1 cttcttatga aaaaaaatca caagtcaaac tagcgttgac aaaattgccg gcaaaaagca
       61 agcataaagg aagtatcctg ataataagtg gtggtcccgg gttaccaggc ataaatcctt
      121 atattaactt tgactggcca gtcacaaatc ttcgtgagtc atgggatatt attggatttg
      181 atcctcgagg cgtcggacag tccactccga caataaactg ccggcaatca gatacagaga
      241 ctcaggaaaa cataaccgaa aagcaacaag tattaaataa aattaatgcc tgtatccata
      301 ataccggagc cgaagtcatt cgccatatcg gctctaacga ggctgtatac gatattgatc
      361 gtattaggca agccttgggg gataaacaac tgacagccgt ggcgtattcg tatggaactc
      421 aaattgcagc cttatatgca gaacgttttc cctacaacgt aagatctatc gttcttgatg
      481 gagtcgtcga tatcgatgac ctggaggaca acttcacatg gcaactcaaa caggcacaga
      541 gttatcagga aacgtttgat cgctttgcat cctggtgtgc gcgtacaaaa agttgcccgc
      601 tttcttcaga cagagataag gcaataactc agttccatga gctattatca aaattacatc
      661 acaaaccttt attagacagt aagggagaaa atatatcttc agatgaactc atatcattaa
      721 caacagacct tctgctatgg cgttcatcat ggccaaccct tgcaactgcc atacgccagt
      781 tctctcaggg gattgtcagt aatgaaattg aaactgcgct cagtgctccg atagcctcag
      841 aagagtcaag cgatgcttcg ggggtaatcc tctgtgtaga tcagggggat gagcaattaa
      901 caccagaaga gcgaaaatcc cgaaaagacg ctcttgcgaa tgccttcccg gctattaact
      961 ttgacaatgg acgttccgat tcacctgatt tttgtgaatt atggccaata catagcgacc
     1021 tgaacaaaac tcgcctgaaa aatactgttc tgccctctgg tttactgttt gtagcacaca
     1081 aatacgaccc aacaacgccc tggattaatg cccgtaagat ggcagagaaa ttttccagcc
     1141 cgttactaac aataaatggt gatgggcata cattagctct caccggagtt aatttatgtg
     1201 tagataaagc agttgtacat cacctgatca ctccacaaaa accagaaaat atatactgcc
     1261 caggaaattc tgaagcagaa atacaataat ttcaa
//
LOCUS       DAZKFU020000542         1294 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZKFU020000542 DAZKFU020000000
VERSION     DAZKFU020000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.544"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..315
                     /locus_tag="ACHOQQ_RS25435"
                     /old_locus_tag="pgaptmp_005087"
     CDS             <1..315
                     /locus_tag="ACHOQQ_RS25435"
                     /old_locus_tag="pgaptmp_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HGU4473195.1"
                     /translation="RTQSRRLKLSTRRARAAQFRDGWVQGIRQVVMPFSVTEEESRLM
                     RLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNASLHHGVGESARQVVPGLMIRE
                     AS"
     gene            312..920
                     /locus_tag="ACHOQQ_RS25440"
                     /old_locus_tag="pgaptmp_005088"
     CDS             312..920
                     /locus_tag="ACHOQQ_RS25440"
                     /old_locus_tag="pgaptmp_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HGU4473196.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            935..>1294
                     /locus_tag="ACHOQQ_RS25445"
                     /old_locus_tag="pgaptmp_005089"
     CDS             935..>1294
                     /locus_tag="ACHOQQ_RS25445"
                     /old_locus_tag="pgaptmp_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HGU4473197.1"
                     /translation="MAEINFQVLRENDAGELTGVATAGRPVARHFDDGLTLEVNRTCT
                     TGERNANSALYGAVWRAAKDMGYRRCITYTRADESGASLRAAGFVRVKELPSGKSWAE
                     SSVALKSTRDLVGSGGVP"
BASE COUNT          293 a          289 c          438 g          274 t
ORIGIN      
        1 cgtacacaga gccggagact gaaactgagc acccgtcgtg cccgggcggc tcagtttcgt
       61 gatggctggg ttcaggggat tcgtcaggtg gtgatgccat tcagtgtgac agaagaggaa
      121 agccggctga tgcgtctctg gatggagcag caggatatgg tgacggtgga ctcccgtgag
      181 atgaaagcct gtcgcggcgg tgacctggcc cgctggcagg ggtatgaggc cgggaaaaat
      241 gcctcactgc atcatggtgt gggggagagt gcccgacagg tggtgccggg tctgatgata
      301 cgggaggcgt catgaataac agcttgtctc aggcaaccaa cggtctgctg atgcaactgg
      361 tgatggacct caagagcggc tacctgcgtc gctgcgaatc tctggggctg gcccgggagg
      421 aaatgcagat gctgcaggga ctgaccattg aggaaattca ttacctgtca aactctgaag
      481 tgtcggttat ccggctggac attaatcatg aaaacctgct gcgtatgctg cagcaggccc
      541 ggacggagca gaaacgtctg cagcgcattg accgggcact ggcgctggga ggctccattg
      601 aactgatggc gttttacttt ggactttcca gtgtggatgt ggcggcgcgc cgtcgcattg
      661 ccggtattga tgttcgcccg ggacgtggca tcacactgag tgatgaggaa agtgcagagc
      721 tgtggcgttt atggcagaag agcggcatca cggatgtgga gagtgcggac ggactggatg
      781 tcatgatgct ggcagctgag caaatgaata tcccgctgac ggcggtatgg catgccgtga
      841 ggggatggca tcagggaact gcggggttta atgatgtatc ggccagtcgc cggaaactga
      901 accgggaaaa agcagaatga caaggacaaa tgacatggcc gaaattaact ttcaggtact
      961 gcgggaaaat gatgcgggtg aattgacagg agtggcgaca gctggcagac ctgttgcccg
     1021 ccactttgac gatgggctga cactggaggt caatcgtacc tgtactactg gtgaacgtaa
     1081 cgctaacagc gcactttatg gcgctgtctg gagggcggca aaagatatgg gctatcgccg
     1141 ttgcattaca tacaccaggg ctgatgaatc cggagcctct cttcgggcag ctggttttgt
     1201 tcgtgtgaaa gaacttcctt caggaaaaag ctgggcggaa tcaagtgtcg ccctgaagag
     1261 tacacgcgat ctggtcggaa gtggtggtgt tcct
//
LOCUS       DAZKFU020000543         1292 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZKFU020000543 DAZKFU020000000
VERSION     DAZKFU020000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.545"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..998
                     /gene="ompT"
                     /locus_tag="ACHOQQ_RS25450"
                     /old_locus_tag="pgaptmp_005090"
     CDS             45..998
                     /gene="ompT"
                     /locus_tag="ACHOQQ_RS25450"
                     /old_locus_tag="pgaptmp_005090"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HGU4473198.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1185..>1292
                     /locus_tag="ACHOQQ_RS25455"
                     /old_locus_tag="pgaptmp_005091"
     CDS             1185..>1292
                     /locus_tag="ACHOQQ_RS25455"
                     /old_locus_tag="pgaptmp_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473199.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFN"
BASE COUNT          412 a          270 c          266 g          344 t
ORIGIN      
        1 aaaatacata ttcaatcatt aaaacgattg aatggagaac ttttatgcgg gcgaaacttc
       61 tgggaatagt tctgacaacc cctattgcga tcagctcttt tgcttctacc gagactatat
      121 cgtttactcc tgacaacata aatgcggaca ttagtcttgg aactctgagc ggaaaaacaa
      181 aagagcgcgt ttatctagct gaagaaggag gccgaaaagt cagtcaactc gactggaaat
      241 tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc cagatatcta
      301 ttggggctgc gggctggact actctcggca gccgaggtgg caatatggtc gatcaggact
      361 ggatggattc cagtaacccc ggaacctgga cagatgaaag taggcaccct gatacacaac
      421 tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac gaacccaatt
      481 accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca gccagaggtg
      541 gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc ttcccgaatg
      601 gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa
      661 gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc tgggtggaag
      721 catctgataa cgatgagcac tatgacccag gaaaaagaat cacttatcgc agtaaagtca
      781 aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaaca cctaacgcaa
      841 aggtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat acttcacttt
      901 atgatcacaa tgataacact tcagactaca gcaaaaatgg tgcaggcata gaaaactata
      961 acttcatcac tactgctggt cttaagtaca ccttttaacg aagttaacca gattttctcc
     1021 ccggtggtat tgtataaccc cggggaattt gtttacagtt acaagtaggc aatatccact
     1081 aattttgtaa gcatttaacc tatgtcataa cgtgtaatct cttaccgcaa cacaagacct
     1141 caatatattc aaatcttatc tttattccga tattcagaga cacaatgcga aaaataatta
     1201 ctcatttcaa agttgtttta acgttacttc taccagtaac cgtatctgcc cagcagatac
     1261 agtggcaatc ctgtatggcc agtcaattca ac
//
LOCUS       DAZKFU020000544         1291 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZKFU020000544 DAZKFU020000000
VERSION     DAZKFU020000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.546"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..153
                     /locus_tag="ACHOQQ_RS25460"
                     /old_locus_tag="pgaptmp_005092"
     CDS             <1..153
                     /locus_tag="ACHOQQ_RS25460"
                     /old_locus_tag="pgaptmp_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HGU4473200.1"
                     /translation="RLTPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTF
                     LGSTNF"
     gene            167..>1291
                     /locus_tag="ACHOQQ_RS25465"
                     /old_locus_tag="pgaptmp_005093"
     CDS             167..>1291
                     /locus_tag="ACHOQQ_RS25465"
                     /old_locus_tag="pgaptmp_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HGU4473201.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAM"
BASE COUNT          313 a          285 c          436 g          257 t
ORIGIN      
        1 agactgacgc cttcagtctc cacgtcctgc gcagtcttcc ctgacggaga gcatgaagcg
       61 gctgaaacag gtaatgctgc ctttgccctg tggcagccct acagttgctg caagaaacgg
      121 ggacagacat tcctgggcag tacgaatttt taagtaaggg gaagtgatga aacagaaaat
      181 gacgatacct gttctggctg tggtgctttg tgcgggtacg gtacaggctg acggaaactt
      241 tttgtccctg ccgaaagtga atgacagtgc gtttggctac ggtaaatccg tggacggcgc
      301 agtatcagac aaactgtttt ataccctggg agggggctcg gtgatatcgc agccggcaac
      361 gcgcagcaat atgcagaaac ttggggtgga tctcgggtgg agcagtgatc tgatgtgtgg
      421 caattttgac ctgaaaacaa cagtgggtaa ccagctgaac gggatcactg atggtttcaa
      481 aaacctgatg ggggatgtga ttcagggggc gagcggagcc gtggcgagtc tgccggcaat
      541 ggtgatacag cgggctaatc cggggctgta tgacatgctg accaacggtg tactgcaggc
      601 gaatgtggca ttcgacaaag cgcagctcaa ttgtcagaac atggcgaaac gcatgatgga
      661 gttcacggac agcaataagt ggacgcaggc ggccatgctc gaggagtaca aaaaaattgt
      721 gaatacgggg gacggtgatg cgatcaaagg ggatgaagca ggtcgtcagg ttaccgggga
      781 ggaggggcaa cgctggatcg gagggcagca gcgtggtgga gcgggacagt ctgctatccg
      841 gccgacacat gatctgacag ctgccggttt caatatgatg aacagtctgc cttcgaccag
      901 tacatccagt gtcagtcccg ggaactgtaa cgggagtgcc tgcatgaaat accgtaatgc
      961 agaggaagct gcccgggcgg tggtgaaggt tctgggggat aaatccatgc ggacctgtgg
     1021 tgatgcaaag gcatgtacca gcggggatgc tgatcagcag ccggggacca gtattgccgg
     1081 cacggggttt gcgccgatgc ttgaagaagc gacgcgggtt aacacggaac tgctggcagg
     1141 tatggtcaat ggtcgtgaca agccgacagc cgggaatctg gcaaaactga aagccgggag
     1201 tctgtctgtg acagcgggag tgatccgtgc cctgcagcgt gatccggaca atggtgccct
     1261 gacgtcgcgt ctggcgggag aactggccat g
//
LOCUS       DAZKFU020000545         1274 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZKFU020000545 DAZKFU020000000
VERSION     DAZKFU020000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.555"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(80..460)
                     /locus_tag="ACHOQQ_RS25470"
                     /old_locus_tag="pgaptmp_005094"
     CDS             complement(80..460)
                     /locus_tag="ACHOQQ_RS25470"
                     /old_locus_tag="pgaptmp_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473202.1"
                     /translation="MSELEKITIPNGAAFPGTDISSLEDTPPTTESSSDGDNLNAQRA
                     ADAKSNRELREKFAEKAYKVAKYGLYWWASVLLLSATGRAMGKEIFSDEVLIAVTAAS
                     TLNLFAAFLGVIRGLFPHAKSHTE"
     gene            complement(460..930)
                     /locus_tag="ACHOQQ_RS25475"
                     /old_locus_tag="pgaptmp_005095"
     CDS             complement(460..930)
                     /locus_tag="ACHOQQ_RS25475"
                     /old_locus_tag="pgaptmp_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HGU4473203.1"
                     /translation="MAYSAAAVANAFIDKAKRGEIKDLTPMKLQKLLFYTQSWHLKLF
                     NGRPLFDDLFARWPYGPVIPHLYHMLKKYGGDEIQEMISIIIDSDGEWKRITPTIQKG
                     DTETRKLIDRIVEVYGSYSGTELSNMTHAPNAAWSKGQPDGGPILHTDMANHIH"
     gene            complement(1078..>1274)
                     /locus_tag="ACHOQQ_RS25480"
                     /old_locus_tag="pgaptmp_005096"
     CDS             complement(1078..>1274)
                     /locus_tag="ACHOQQ_RS25480"
                     /old_locus_tag="pgaptmp_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473204.1"
                     /translation="FENNVLYGKLAFIRDLVTYEAKTLADLEQEFKTSVDLYLHSCVD
                     DGKEPAPPFKNLPVAYLLSP"
BASE COUNT          353 a          273 c          290 g          358 t
ORIGIN      
        1 gattaattta tttatgtaaa aaatataagc gaggataatg aagatggagg atgcgtaaag
       61 aaaaaccggc gcgggccggt tactctgtgt gagatttggc gtgagggaaa agcccccgga
      121 taacaccaag aaaagcagcg aacagattca gagttgaagc agcggtcacc gcaatgagca
      181 cttcatctga aaagatttct ttccccatgg cgcggcctgt ggcagacaac aacaaaacac
      241 tagcccacca gtaaagccca tacttggcaa ctttataggc tttttctgca aatttttctc
      301 ttaattccct gttagatttt gcatctgctg cgcgctgggc gtttaaatta tctccatctg
      361 atgaagattc tgttgttggt ggcgtatctt caaggcttga tatatctgtg cctggaaatg
      421 ctgctccgtt cgggattgtg attttttcaa gttcagacat caatgaatgt ggtttgccat
      481 atcagtatga agaattgggc caccatcagg ttgaccttta ctccaggccg cgtttggtgc
      541 atgggtcatg ttagaaagct cagttccgct gtagctgccg tatacctcaa caattctgtc
      601 gatcagcttc ctggtttctg tgtcaccctt ctgtattgtc ggtgtgattc gtttccattc
      661 cccatctgaa tcaatgatga tgctgatcat ttcctgtatt tcatcgcccc catatttctt
      721 taacatgtgg tacaggtgcg gtattacagg cccgtaaggc cagcgcgcaa acagatcgtc
      781 gaataatggc cttccgttga atagtttcag atgccacgac tgagtataga acaacaattt
      841 ttgtagcttc atcggagtta aatctttgat ttctccccgt ttagccttat caataaaggc
      901 gttagctacg gcagcagctg aatatgccat gctctactcc gcattttgcg aaattaaatg
      961 tatcaccgga gagaatacaa caaaaagcaa attagcaaaa ctgatagaga gggcaggaaa
     1021 caaaaattag aggtgaacgg cagtcacgag gtgaagaaag tccggtgagt tggacgacta
     1081 tggagaaagc agataagcta ctggcaagtt tttaaagggg ggggcaggtt cctttccatc
     1141 atccacacaa gagtgcaagt acagatcaac cgatgtttta aactcctgtt ccaggtctgc
     1201 taatgttttt gcctcataag tcaccagatc acggataaac gccagctttc catacaggac
     1261 attattttca aaat
//
LOCUS       DAZKFU020000546         1272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZKFU020000546 DAZKFU020000000
VERSION     DAZKFU020000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.556"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..1072)
                     /locus_tag="ACHOQQ_RS25485"
                     /old_locus_tag="pgaptmp_005097"
                     /pseudo
     CDS             complement(1..1072)
                     /locus_tag="ACHOQQ_RS25485"
                     /old_locus_tag="pgaptmp_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          216 a          428 c          323 g          305 t
ORIGIN      
        1 ctgttactgt tgtggcagaa ccaagaacca gtgtctcacc agacaaccca agaggatccc
       61 cccgctgcac cgggttcagc ggatacgcat acaggttcca tccccccttc agcccaatgg
      121 gatcctgggt gatatagcgt ccctgcaacg gatcgtagta ccggttccgg ttatagtaca
      181 gccccgactc ctcatcatac tgctgccccg gcagacggag tgactgttga aggtgctgcg
      241 cgctggtttc gcccagcagg tttccccact cgtcatactc cccctgccac gccgtttccc
      301 cttccgggct gatgagcgcc agcggcagtc cccggtggtc gcagtggtaa agatgaagtt
      361 tcctctccgg gatgtattcc gcctccagct gtgcggccat ctgctccgcc gtcagcccgc
      421 actgcgcaag ccatgcctca ctctccgcac tcaccgctcc cgcgcgcagt tcccgctcca
      481 gccttcccag catcaccgcc agctccgccg gtagcgtcac ccccgtgtcc tcctgcaaca
      541 cctccgccag gctacggtgc cgcgccttcg cctgttcgca attctctgtt tcgattctga
      601 tgagtggtgt gaagctcccc ggctggtata ccgtctggat acgcgtggtg tcggtctgta
      661 ccgtcgtcag cctgtcgccg tcccacccgt accacgtctc ctccggttta cgcgacagcg
      721 acatccaccc cgtcaggtca cgctcccgcc gccacacccg ttttcccgtc cggcggccca
      781 gcgggtcata gaggtaccgg ctctccacct gtggttcgcc gtgctgtatc cgcgtgtaga
      841 acaccaggcg gtgctggctg tcgtagtgat agtggtgggt gcgctcgtcg tgcatccgga
      901 taaccccttc cgggatacgg tccgtcttct ccgccagcct gccgtattca tcgtagcggt
      961 agacatagtg cgcatcttcc gcgatgcggt tatccggcca cgccgtgagc gtgctgtccg
     1021 gatgcagttc cgggtccggc agccggtttc ccgccgggtc tgttgcatac gggaaattcc
     1081 cttgcagggc gtccacaaca tgtgcatatt tcctgcacat gctgtggttt tgtcatacaa
     1141 ggagcaacaa tactagttaa tgattcataa aaaattttaa cggggtgatt tttcatgcga
     1201 ttatcgtcct aattaacgcc acctcactta cagattattt cattcgttac aatcttttga
     1261 ttgcattaca cc
//
LOCUS       DAZKFU020000547         1268 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZKFU020000547 DAZKFU020000000
VERSION     DAZKFU020000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.557"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            188..>1268
                     /locus_tag="ACHOQQ_RS25490"
                     /old_locus_tag="pgaptmp_005098"
     CDS             188..>1268
                     /locus_tag="ACHOQQ_RS25490"
                     /old_locus_tag="pgaptmp_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4473205.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDVSKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPG"
BASE COUNT          400 a          235 c          280 g          353 t
ORIGIN      
        1 tcatcataaa taaaaagact attgtttatc atattgttct cagcattata tgattattta
       61 tcctgataac tctcctatgt tgtatgttta tatgattttc cttgaaacat ataatgcaaa
      121 ttttcgattt attttccatc attaatccag ataaacaaca aactaatagt atgcaaggag
      181 acattatttg tttcgccagg atgctttaga aaacagaaaa atgaagtggc agggacgggc
      241 aatattactt cccggaatac cactgtggtt aatcatgctg ggaagcattg tgtttattac
      301 ggcatttctg atgttcatca ttgttggtac ctatagccgc cgtgttaatg tcagtggtga
      361 ggtcacaacc tggccaagag ctgtcaatat atattcaggt gtacagggat ttgttgtcag
      421 gcagtttgtt catgaaggac agttgataaa aaaaggggat cctgtttatc tgattgacgt
      481 cagtaaaagt acacgtagtg gtattgtcac tgataatcat cgtcgggata tagaaaacca
      541 gctggttcgt gtggacaaca ttatttcccg tctggaagaa agtaaaaaaa taacgctgga
      601 taccctggaa aaacaacgtc tgcaatacac agatgcgttt cgtcgctcat cagatattat
      661 acagcgtgca gaggaaggga taaaaataat gaaaaacaat atggagaatt acagaaacta
      721 tcaggcaaaa gggctgatta ataaagatca gttaactaac caggtggcat tatattatca
      781 gcaacaaaac aatcttctca gcctgagcgg acagaacgaa cagaatgccc tgcagataac
      841 cactctggag agtcagattc agactcaggc tgcagatttt gataaccgta tctaccagat
      901 ggaactgcaa cggtacgagt tacagaaaga actggttaac actgatgtgg agggcgaaat
      961 cattatccgg gcgttgactg acgggaaagt tgactccctg agtgtcactg tcgggcaaat
     1021 ggtcaatacc ggagacagcc ttctgcaggt tattcctgag aacattgaaa actattatct
     1081 tattctctgg gtcccgaatg atgctgttcc ttatatttcg gctggtgaca aagtgaatat
     1141 tcgttatgag gcctttccgg cagaaaaatt tgggcagttc tctgctacgg ttaaaactat
     1201 atccaggact cctgcgtcaa cacaggaaat gttgacctat aagggagcac cacagaatac
     1261 gccgggcg
//
LOCUS       DAZKFU020000548         1255 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZKFU020000548 DAZKFU020000000
VERSION     DAZKFU020000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.558"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            183..644
                     /locus_tag="ACHOQQ_RS25495"
                     /old_locus_tag="pgaptmp_005099"
                     /pseudo
     CDS             183..644
                     /locus_tag="ACHOQQ_RS25495"
                     /old_locus_tag="pgaptmp_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            747..1130
                     /locus_tag="ACHOQQ_RS25500"
                     /old_locus_tag="pgaptmp_005100"
     CDS             747..1130
                     /locus_tag="ACHOQQ_RS25500"
                     /old_locus_tag="pgaptmp_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473206.1"
                     /translation="MNYQAVSELITSSNHNVLIVGDSASEVDSFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVAVREQDMKKMEAEFGRIIKIH"
BASE COUNT          368 a          216 c          298 g          373 t
ORIGIN      
        1 tgcatgatta attcatgaga ccttagtaac acatggctat atgtgtattc agccactgaa
       61 tcttatttta ctgaatagac cacaatttaa cggtttattt ttggcgtgat gagtgaggca
      121 ttattcatca cggaatggaa ttcctgcaat cacaaaaagt aatcagtgac ggagagagta
      181 aaatgaagaa atatatacca gtggtattgt ttgttttttc atatcaggtt ttttctgctg
      241 atattcatgg acgggtcgtg cgtgttctgg atggcgatac cattgacgtc atgttatctc
      301 agcatccggt gcgtgtccgt cttgttaata ttgatgcacc tgaaaagaaa caggactatg
      361 gacggtggac tgaaaaaata atgtaatctc tggttgcggg aaaaacggtt actgtcacat
      421 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
      481 atgttaatca gtttatggtc cgtgcaggcg ctgcatgggt ttatgagcag tacaacaccg
      541 atcctgttct tcccgtcctg caggaagaag cacgacagca gaaacgtggt ctgtgggcag
      601 acagcgcgcc tgttccgccc tgggtatggc gctcacggaa ataatgatat ttctgtggct
      661 attgagttct cattgaaatg cgatatttaa aagtatgaag catattacat tattaactta
      721 tttctctgtt aatgaaaggt gaacaaatga actatcaggc agtatcagaa ttaatcacat
      781 cgtctaatca taatgttctt attgtggggg actcagcttc tgaagttgat agctttttaa
      841 ataaactgaa tattactgat tataagtatt atgatttcag ccagatttac agttgttctg
      901 acagaacatt aaatgattat gcggttattt ttatcaggga tgcactgaac gccagtgagc
      961 atattattat ttttaattgt acaggatggc ctgatttgaa taatgaatct gccgtcatgc
     1021 agtttgccag agtggcacga aaatcaggaa aacaactgat agtcgcagta agagaacagg
     1081 atatgaaaaa aatggaagca gagtttggaa ggatcataaa aatccactga cgccttcgcg
     1141 ttgctcagtt gtccaacccc ggggacggga aaaagcaagt tttccccgcc cccggcgttt
     1201 ctgtagtgaa aagcgggaaa tggagggaat ggtcagtaac agggaatgtg gtatt
//
LOCUS       DAZKFU020000549         1251 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZKFU020000549 DAZKFU020000000
VERSION     DAZKFU020000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.559"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1251
                     /locus_tag="ACHOQQ_RS25505"
                     /old_locus_tag="pgaptmp_005101"
     CDS             <1..>1251
                     /locus_tag="ACHOQQ_RS25505"
                     /old_locus_tag="pgaptmp_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGU4473207.1"
                     /translation="EVIEKTGRGDDDLLTWLLTYQPLAHMALKLGITPPCWPSLEIRP
                     DRRTEPDPERVPGAVMRLSCATWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEA
                     LLDWREKRQRTRDFLKAWDIENEEGERFSLEDIYWAGLSNPRNRRNEMMTCARGMEQV
                     AEARGDVAFFVTVTAPSRFHSVNDDGSLNPKYCGATVRDASDYLVYRFFAAVRRTIDK
                     AGLGWYGVRTVEPHHDGTPHWHMLVFTSQENEARITEIMRNAAIREDRAELGDDISPR
                     FKCEKIDPTKGTPTSYIATYIGKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAI
                     GWAGLHRIHQFQFFGIPPRQVWRELRRLAGQMARNPAAPQRLDHDDIDAILAAADVGC
                     FATYITRQGGVLIPRNTYLVRTAY"
BASE COUNT          286 a          389 c          354 g          222 t
ORIGIN      
        1 tgaggtcatc gaaaagaccg gcaggggtga cgatgacctg ctcacctggt tactgaccta
       61 ccagccactg gcacacatgg cgttaaaact gggtatcacg ccaccctgct ggccgtcgct
      121 ggaaatcagg cccgaccgcc gcaccgagcc cgacccggaa cgggtgccgg gtgcggtcat
      181 gcgcctttcc tgtgcgacat ggtggaacag tcagttaaga cacctccggg acatctggcg
      241 tgaagaactg ttacgcgccg caggtctggt gtcacgtaag acatcggtat atatcagcca
      301 tgaagcgtta ctggactggc gcgaaaaacg ccagcgcacc cgcgattttc tgaaggcctg
      361 ggacatcgag aacgaagagg gagaacggtt cagccttgag gatatctact gggcaggtct
      421 ctctaatccg cgtaaccgcc gtaacgagat gatgacctgc gcccggggaa tggagcaggt
      481 cgccgaagca cgcggcgacg tggcgttttt cgtcaccgtc acagcccctt cccgttttca
      541 cagcgtgaac gatgacggca gcctgaaccc gaaatactgc ggagcaacgg tacgcgatgc
      601 cagtgattat ctggtttacc gctttttcgc cgccgtccgc aggaccatcg acaaggccgg
      661 gctgggctgg tacggcgtgc gcaccgtgga gccccaccac gacggtacac cgcactggca
      721 catgctggta ttcaccagcc aggaaaacga agcccgtatc actgaaatca tgcgcaacgc
      781 cgccatcagg gaggaccgcg cagagctggg tgatgatatc tccccgcgtt ttaaatgcga
      841 aaaaatcgac ccgacaaaag gcacaccgac aagctatatc gcgacctata tcggtaagaa
      901 tctggacgcc agcgccttca tggggaatga cccgaaaacc ggcaaacctt acgtcgataa
      961 ggaatcaggc aaaaccatgg ccgaaaccgt ggagaacgcc atcggctggg caggccttca
     1021 ccgcatccac cagttccagt tcttcggtat ccctccccgc caggtatggc gcgaactccg
     1081 ccgtcttgcc gggcaaatgg cccgtaaccc ggctgccccg cagcgtctgg accatgacga
     1141 cattgacgcc atactggccg ccgccgacgt gggatgtttt gccacctata tcacccgcca
     1201 gggtggtgtg ctcattcccc gcaacacgta cctggtccgc accgcatacg a
//
LOCUS       DAZKFU020000550         1247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZKFU020000550 DAZKFU020000000
VERSION     DAZKFU020000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.560"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..455
                     /locus_tag="ACHOQQ_RS25510"
                     /old_locus_tag="pgaptmp_005102"
     CDS             <1..455
                     /locus_tag="ACHOQQ_RS25510"
                     /old_locus_tag="pgaptmp_005102"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4473208.1"
                     /translation="QSASAKKTPYCVMLMDIDHFKKVNDTWGHPIGDQVIKTVVNIIG
                     KSIRPDDLFARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKVTI
                     SIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
     gene            553..>1247
                     /locus_tag="ACHOQQ_RS25515"
                     /old_locus_tag="pgaptmp_005103"
     CDS             553..>1247
                     /locus_tag="ACHOQQ_RS25515"
                     /old_locus_tag="pgaptmp_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HGU4473209.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGVNNNDRLYML"
BASE COUNT          383 a          219 c          280 g          365 t
ORIGIN      
        1 ttcaatcagc atcggccaaa aaaacgcctt attgcgtcat gcttatggat atcgaccact
       61 tcaaaaaagt caacgacacc tgggggcatc cgattggcga tcaggtgata aaaacagtgg
      121 tgaatatcat tgggaaaagc atacggccag atgacctttt tgcgcgcgtc ggcggcgaag
      181 agtttggcgt cttgctgacg gacatcgata ctgaacgtgc gaaagcttta gcggaaagga
      241 ttcgggaaaa tgttgagcgt ttaactggtg ataatcctga atacgccata ccacaaaaag
      301 tgacgattag tattggcgct gttgtgactc agggaaatgc gttaaacccg aatgagatct
      361 atcgactggc tgataacgca ctatacgagg cgaaagagac cgggcgtaat aaggtggttg
      421 tgagggatgt ggtgaatttt tgtgagtcac cataaagcgg cattttagcg ggtgattttg
      481 gtaagcgcta aatagctatt cgcagacatg cgtaacaatt ttcagaaata tcatagtact
      541 atatgatctt aaatgcttat cattgatcag tctgctatca cccgctttta ctggcaattg
      601 tactataatt tcttaggttg catcaccctg atgcgtgggg aaacatgtat ggtattcaac
      661 aaaaaaatgt ttgtactcat tataatacca ggcattctgg gggtgttact gtcgtttgct
      721 atgtcagtat ttcaaatgaa tcgagatacg acaattaccg ccggtattct gttaaaacag
      781 cttgataatg ttacgcaaat tgtcaaacat acgacaaagc ttacttctat tctggtgatg
      841 aaaccctgca aagatatcct ggaacaattg attgctaacg gagcattgac cccctatgtt
      901 cgaacaacgg gtcttataga aaataatttc caaatatgtt cttcagttag cggtttcaaa
      961 aaaatgaatg ttaacgatgt ttacggtacg agctttcaca ataaaaataa agaatcacgc
     1021 atagtatcaa ttagcggtac aagttttgta ccaggtaaaa cagccatagt gttccttatg
     1081 ccaataggga atgatatgac agcttttagt attgtagagt cacgatatat ctatgatctg
     1141 atggatgttc ttgatgatga gaatgatgat tcattttctc ttcgctttac tgaagggcct
     1201 gcaattatta gtggtgtaaa taataatgac aggttatata tgttaaa
//
LOCUS       DAZKFU020000551         1245 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZKFU020000551 DAZKFU020000000
VERSION     DAZKFU020000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.561"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..497
                     /locus_tag="ACHOQQ_RS25520"
                     /old_locus_tag="pgaptmp_005104"
     CDS             <1..497
                     /locus_tag="ACHOQQ_RS25520"
                     /old_locus_tag="pgaptmp_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4473210.1"
                     /translation="EHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQHLSE
                     TKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYVFEQF
                     EIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSETDGV
                     EAKK"
     gene            complement(557..1195)
                     /gene="ompW"
                     /locus_tag="ACHOQQ_RS25525"
                     /old_locus_tag="pgaptmp_005105"
     CDS             complement(557..1195)
                     /gene="ompW"
                     /locus_tag="ACHOQQ_RS25525"
                     /old_locus_tag="pgaptmp_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGU4473211.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          355 a          301 c          264 g          325 t
ORIGIN      
        1 ttgaacatta tcatgactgg ttacgtgacg cccatgcaat ggaaaagcaa gccgaatcta
       61 tgcttgaatc catggccagc cgtatagata attatcctga actacgtgct cgtattgaac
      121 aacatcttag tgaaaccaaa aaccagattg ttcaactgga aactattctt gatcgtaatg
      181 acatttcacg ttcagtcatt aaagattcca tgagtaaaat ggctgcgctt gggcagtcaa
      241 tcggtggtat attcccttct gatgaaatag tcaaaggttc tattagcgga tatgtcttcg
      301 agcaatttga aatcgcctgt tacacatctc tattagcagc agcaaaaaat gccggtgata
      361 cagcctcaat tccgatcatc gaagcgattt taaatgagga aaagcaaatg gccgactggc
      421 tgattcagca tattccgcaa acaactgaga aatttttaat tcgctctgaa actgatggcg
      481 tagaagcgaa gaaataatac cctttatgcc atgtccttat tgaccccgtt tattacgggg
      541 tcgtttttgt gcggaattaa aaacgatatc ctgctgagaa cataaacacc cacggatcga
      601 ggcgtacgct atcatgttgc tgtgcaccgc ccagtttata attggcggtg gtatcgatat
      661 ccatgtacca cactgacata ttaaccaacc agtcacggtt aatcagataa tcaaccccca
      721 cctgcccggc agctccccag gaatctttca gactgagatc ggaaagcccc gcctctttgc
      781 catgatcgtt aaatccatta tcaaagaagg tggtgtagtt aatacctgcc ccaacataag
      841 gacggaattt gctgctggca tcaccaaaat accactgcgc catcagtgtt ggcggcagat
      901 gatgaacggt tgcaatatcg ccggtcgccc gggtgccgat tttatggcgg aacggcgtcg
      961 ctgccagtaa ttccacacca atgttgtcgg tcgccatata agtaaacgtc aggcccagtt
     1021 gagtgttatt ggtcacgctg aatccaccca gacttcctaa cgtaccacca gcaccttctg
     1081 ttggacgtac ggttgcagaa cctgcacgca taaaaaattc gcctgcttca tgcgcaaagg
     1141 cactgccaga gagaagtgtt gttactgcca aagccgccac tgttaacttt ttcatatccg
     1201 ctccgtcgtt atggttataa aaacgtgaca aatataccta cattg
//
LOCUS       DAZKFU020000552         1237 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZKFU020000552 DAZKFU020000000
VERSION     DAZKFU020000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.562"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1237
                     /locus_tag="ACHOQQ_RS25530"
                     /old_locus_tag="pgaptmp_005106"
     rRNA            <1..>1237
                     /locus_tag="ACHOQQ_RS25530"
                     /old_locus_tag="pgaptmp_005106"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          310 a          286 c          391 g          250 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacaga actttccaga
      721 gatggattgg tgccttcggg aactgtgaga caggtgctgc atggctgtcg tcagctcgtg
      781 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
      841 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
      901 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
      961 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1021 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1081 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1141 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1201 tcgtaacaag gtaaccgtag gggaacctgc ggttgga
//
LOCUS       DAZKFU020000553         1237 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZKFU020000553 DAZKFU020000000
VERSION     DAZKFU020000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.563"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..948
                     /locus_tag="ACHOQQ_RS25535"
                     /old_locus_tag="pgaptmp_005107"
     CDS             <1..948
                     /locus_tag="ACHOQQ_RS25535"
                     /old_locus_tag="pgaptmp_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HGU4473212.1"
                     /translation="GLTRTYFSTPSGETHEAYQFWTGDRWNSEQPRSKRSEFDVSHAH
                     LKGGVLFPDETWRQIVTIEDAVANGWRYTNIGVIRGENTESEFRNLYMCEFVRDGEAA
                     FSLSALTGCGVDGYDEWPDWKPFAARPMGVREVWLGYDANGSSGKGDCGALSVCVPPL
                     VAGGRFRTVETVRVQGMEFEQQAALIEEYTQKYNVKHIGIDVTGGNGEGVYQIVKKFF
                     PMAVPYNFNIASKRALVMKMLQVVRAGRWEYDRSERDLIQAFASVRKIQTPGGYITYD
                     TDRSRGVSHGDLAWATMLAVINEPIGAEREDNRGFAMEF"
     gene            954..>1237
                     /locus_tag="ACHOQQ_RS25540"
                     /old_locus_tag="pgaptmp_005108"
     CDS             954..>1237
                     /locus_tag="ACHOQQ_RS25540"
                     /old_locus_tag="pgaptmp_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="gpQ; Q protein; structural capsid protein;
                     bacteriophage P2-like virions include a head and a tail; Q
                     is a connector or portal protein that joins phage head and
                     tail; Q, P and M proteins are needed to package DNA into
                     proheads and for the conversion of proheads to capsids;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4473213.1"
                     /translation="MSKRKKYTGKRGQDSLHDALKSTPALSAFTFDGPYEVSRMDLLD
                     NMSCADNGRYFDTPMDWNAIARASRRASWHQSALYFKRNALNGCFVPHPLL"
BASE COUNT          283 a          304 c          372 g          278 t
ORIGIN      
        1 gggcttacgc gaacctactt ttcaacaccg tcaggtgaaa cacatgaggc gtatcagttc
       61 tggacggggg accgctggaa cagcgaacag ccacgctcaa aacgcagtga gtttgatgtt
      121 tcacatgcgc atctgaaggg gggagtgctc tttcctgacg aaacatggcg gcagattgtc
      181 actattgagg atgctgtcgc taacggatgg cggtacacca atattggtgt tattcgcggc
      241 gaaaatacag aaagtgaatt tcgtaatctg tacatgtgcg aatttgtccg tgatggtgag
      301 gcagccttca gcctgagtgc gctcaccggc tgcggtgttg acggctatga cgaatggcct
      361 gactggaaac cttttgctgc ccgccctatg ggggtgaggg aggtctggct tgggtatgac
      421 gccaacggca gcagcgggaa gggggactgc ggggcactgt cggtgtgcgt gccgcccctt
      481 gttgccggtg ggcgttttcg taccgtggag acggtcaggg tgcagggtat ggagtttgaa
      541 cagcaggccg cactgattga ggaatacacc caaaaatata acgtgaaaca tatcggcatt
      601 gatgtgaccg gcgggaacgg ggagggggtt tatcagattg tgaaaaagtt tttcccgatg
      661 gccgtaccgt acaacttcaa tatcgcctcc aagcgggcac tggtcatgaa aatgcttcag
      721 gtggtgcgtg ccggacgctg ggagtatgac cgcagtgaac gtgacctgat ccaggcattc
      781 gcatcggtgc gaaaaataca gacacccggc ggctatatca cctatgacac cgaccgctcc
      841 cgcggtgtga gtcatgggga tcttgcctgg gcaaccatgc ttgctgttat caatgagcca
      901 attggtgcgg agcgtgagga taatcgcggc tttgcaatgg agttctgaac acaatgagta
      961 aacgcaaaaa atacaccgga aagcgggggc aggattcgct acacgatgca ctgaaatcaa
     1021 cgcctgcact cagcgcattc acctttgatg ggccctatga agtcagccgc atggatctcc
     1081 tggataacat gtcatgtgca gataacgggc gctactttga tacccccatg gactggaacg
     1141 ctatcgcccg tgcatcccgt cgtgcgtcct ggcatcagtc ggctttgtac ttcaagcgca
     1201 acgccctgaa cgggtgcttt gttcctcacc ctctgct
//
LOCUS       DAZKFU020000554         1231 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZKFU020000554 DAZKFU020000000
VERSION     DAZKFU020000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.564"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..312)
                     /locus_tag="ACHOQQ_RS25545"
                     /old_locus_tag="pgaptmp_005109"
     CDS             complement(<1..312)
                     /locus_tag="ACHOQQ_RS25545"
                     /old_locus_tag="pgaptmp_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HGU4473214.1"
                     /translation="MGSKNTPERKKFRVAVSGSTIDGREISGEMLRQAAENYDPSVYA
                     SRVNIEHVLSMWPSSELCAVGDLIALSTDEITEGKLAGRTALYAEIEPTDRMKNMIAD
                     GK"
     gene            468..>1231
                     /locus_tag="ACHOQQ_RS25550"
                     /old_locus_tag="pgaptmp_005110"
     CDS             468..>1231
                     /locus_tag="ACHOQQ_RS25550"
                     /old_locus_tag="pgaptmp_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HGU4473215.1"
                     /translation="MIQDVFIRMRAKQLYWQGYTPADIAQLMGINQNTIYSWKKRDEW
                     DATPPIQRVTQSIDARLIQLTAKPDKSGGDFKEIDLLTRQLKKLNEGQGEQAVSGKKP
                     RRRKLKNHFTEEQIIALRERTLRTLACHQREWYENRHHRNRMILKSRQIGATWYFARE
                     ALLDALRDDVSAGYQRNQIFLSASRRQAYQFKHFIQQAALEVDVELKGGDKIVLSNGA
                     ELHFLGTSAATAQSYTGHLKFDEFFWVSNFVELRKVA"
BASE COUNT          295 a          303 c          334 g          299 t
ORIGIN      
        1 tttcccgtca gcaatcatgt ttttcatgcg gtcggtgggt tcaatttcgg catataacgc
       61 tgtacgccct gccagtttcc cttcggtgat ttcatcagtt gacagagcga tcagatcacc
      121 cactgcgcag agttcactgg atggccacat cgaaaggacg tgctcaatgt tcacccggga
      181 ggcgtaaacg gacgggtcat agttttctgc ggcctgacgc agcatttcac cagaaatttc
      241 acgtccgtcg atggttgaac cggaaacggc aacgcgaaat tttttgcgct ctggcgtgtt
      301 tttgcttccc atggaaattc tccataacct ggtcgttttt cccatgatgg cggcagataa
      361 cggtgtgtct caaggtgctg aagttgtatg gcgacgggga caacacgggc aggtatcagg
      421 cggtattgct gaggcttaat cttgccctgt taatgtcagg gggtgcaatg atccaggatg
      481 tctttattcg tatgcgggcg aaacagcttt actggcaggg gtataccccg gcggatatcg
      541 ctcagctgat gggaataaac cagaacacga tttattcgtg gaaaaaacgc gatgagtggg
      601 atgcaactcc cccgatccag cgggtcaccc agtccattga tgcacgcctg atacagctca
      661 ccgccaaacc ggataaaagc ggcggtgact ttaaggaaat tgacctgctg acgcgtcagc
      721 tgaaaaaact gaacgagggg cagggtgaac aggctgtttc cggtaagaag ccccgcaggc
      781 gcaaactgaa aaatcacttt acggaagagc aaatcatcgc cctgcgtgaa agaaccctcc
      841 gaacactggc ctgccatcag cgtgaatggt atgaaaaccg ccaccaccgt aatcgtatga
      901 tcctgaagtc gcgtcagatt ggtgcgacct ggtattttgc gcgcgaggct ctccttgatg
      961 cactgcgtga tgatgtctcc gcagggtacc agcgcaacca gatatttttg tccgcgtccc
     1021 gtcgtcaggc atatcagttc aaacacttta ttcagcaggc ggcgctggaa gttgatgtcg
     1081 aactgaaagg gggggacaag attgttttgt ctaacggtgc agagcttcac ttccttggca
     1141 catcggcagc aaccgctcag tcgtataccg gacaccttaa atttgatgaa tttttctggg
     1201 tcagtaactt tgtggaactc aggaaggttg c
//
LOCUS       DAZKFU020000555         1226 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZKFU020000555 DAZKFU020000000
VERSION     DAZKFU020000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.565"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(33..800)
                     /gene="sctT"
                     /locus_tag="ACHOQQ_RS25555"
                     /old_locus_tag="pgaptmp_005111"
     CDS             complement(33..800)
                     /gene="sctT"
                     /locus_tag="ACHOQQ_RS25555"
                     /old_locus_tag="pgaptmp_005111"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HGU4473216.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLGIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAEREMPLSFFPEQLQLYIEK"
     gene            complement(802..1062)
                     /locus_tag="ACHOQQ_RS25560"
                     /old_locus_tag="pgaptmp_005112"
     CDS             complement(802..1062)
                     /locus_tag="ACHOQQ_RS25560"
                     /old_locus_tag="pgaptmp_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4473217.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1071..>1226)
                     /locus_tag="ACHOQQ_RS25565"
                     /old_locus_tag="pgaptmp_005113"
     CDS             complement(1071..>1226)
                     /locus_tag="ACHOQQ_RS25565"
                     /old_locus_tag="pgaptmp_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4473218.1"
                     /translation="ISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQY
                     FDLSINP"
BASE COUNT          422 a          252 c          221 g          331 t
ORIGIN      
        1 tggcttttca gttttattgg ccatgaaaca ctctatttct caatgtatag ttgcaattgt
       61 tctgggaaaa aggataacgg catttctcgt tcagccagta ttgggccaaa ataaataatt
      121 aatattaaaa atgccagcgc acttttgaca gtcagtgaaa tggcaaaagc atttagttgt
      181 gaagcataac gtgccagcaa tcccaggaca gcctcaccgc ccagcataac ggcaatcacg
      241 gggcttgcgt aaacgatagt atgagtcatt atgttattaa tatatgagaa taaaggctct
      301 aatttaggaa aattaaaatt acctgaaggc cataaattat aactttgcca aagagtttcc
      361 agaataaagt tcattccacc gttagtaaga taaaccgcag cagagaaaag gttaaataat
      421 cttgctaatt ccgaggtatc gacacctgta gccggatcca aagtactact taatgttgca
      481 ccgcgttggt tatcgataaa gctacctata ccaaggaaaa tccaaaatgg ggatgctaat
      541 aagcatgcaa taaacagccc tatgataatt tctctggcag cgaacaaagc aatatcgatt
      601 tcatttaagt tcaataattc atagtggtaa tgctgaacta atgcacatga taccactatt
      661 attattggat gacggactac tgttggtata ttcccactgg cgaaaaatgg taacaaatag
      721 aaagtgggtg ccaatcggca aaaaccgagc gctgtagcag ccagcagact atgcaactga
      781 tagagtatcg cctcacccat gctatcctct cgcgaatgct aaattcagca tttcaatacc
      841 aaaagaatat aatttttcgc cataccatcc gctcattaaa aaaaagcaaa tacttacgca
      901 taataatttc acgccaaatg gcaacgtctg ttcttgcaac tgtgttaccg tttgaaaaag
      961 ccctactaat aaaccaacaa atgtcgcaac tgcgataggt cctgcagaca taactaaaat
     1021 cagatacagc gccctattcc ctgcaaaaac aatatcgtcc atattttttc tcagggatta
     1081 atggataggt caaaatattg taaaatgagt ccttttgata acattgtcca accgtccata
     1141 gcgacaaaca gaattaattt tatcggtgta gatatggtta ccgggctcat catcatcata
     1201 cctaacgtta acaaaacgct ggatat
//
LOCUS       DAZKFU020000556         1222 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZKFU020000556 DAZKFU020000000
VERSION     DAZKFU020000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.566"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..1200
                     /locus_tag="ACHOQQ_RS25570"
                     /old_locus_tag="pgaptmp_005114"
     CDS             join(44..293,293..1200)
                     /locus_tag="ACHOQQ_RS25570"
                     /old_locus_tag="pgaptmp_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HGU4473219.1"
                     /translation="MSRKIQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDIFKKSNSVFCTGVAEKYALI
                     EQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGT
                     RRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFA
                     PTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALR
                     SQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE
                     SLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          358 a          287 c          311 g          266 t
ORIGIN      
        1 gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaaatcca
       61 acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa atcaactttc
      121 gatcagtgaa ggcgcttccc gattatctct tcctgaaggc actttaggac aatgggttac
      181 cgccgccaga aaagggctcg gtactcctgg ttcccgcacg gtggctgaac tggaatctga
      241 aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt gagcgagata tattaaaaaa
      301 agcaacagcg tattttgcac aggagtcgct gaaaaatacg cgttaatcga acaatggcga
      361 caacaatttc ccattgaagc gatgtgtcag gtatttggtg tatccaggag cggttattac
      421 aactgggtac agcatgaacc ctcagacaga aaacaaagtg atgagcggct aaaactggag
      481 attaaggtgg cacatatccg cactcgcgaa acatatggaa cccggcggct ccagacggag
      541 ctggcagaga atggcatcat cgttggtcgt gaccgactgg cacgtcttcg taaggagcta
      601 aggctacgct gtaagcagaa acgcaagttc agagcgacta cgaactcgaa ccacaatctg
      661 ccagttgcgc caaatctgct gaaccagacg ttcgctccta cagcaccaaa tcaggtctgg
      721 gtggcggacc tgacgtatgt tgccacacag gagggatggt tgtacctcgc tggcatcaaa
      781 gatgtttata cgtgcgaaat tgtcggctac gccatgggag agcgcatgac aaaagagctg
      841 acaggtaaag ccctgtttat ggcgctcagg agccagcgcc cacctgccgg gctaatccac
      901 cactccgatc gtggttcaca gtactgcgca tacgattacc gggtcataca ggagcagttt
      961 ggtctgaaaa catcaatgtc gcgtaaaggt aactgttacg acaacgctcc gatggaaagc
     1021 ttctggggaa cgctgaaaaa tgagagcctg agccactatc gttttaataa ccgggatgaa
     1081 gccatctcag taatacggga atacattgag attttctaca atcgtcagcg tcgtcactct
     1141 cgtctgggga atatctcccc ggcagccttc agggaaaaat atcatcagat ggctgcttaa
     1201 aaaaagaaca aatggtagtg tc
//
LOCUS       DAZKFU020000557         1205 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZKFU020000557 DAZKFU020000000
VERSION     DAZKFU020000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.567"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..>1205
                     /locus_tag="ACHOQQ_RS25575"
                     /old_locus_tag="pgaptmp_005115"
     CDS             52..>1205
                     /locus_tag="ACHOQQ_RS25575"
                     /old_locus_tag="pgaptmp_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1414 family transposase"
                     /protein_id="HGU4473220.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKLSVEAALNAEMT
                     HHLGYEKNQSRPGANSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTRITG
                     MDNQILSLYAKGMTTREIATAFKELYDADVSPALISKVTDAVMEQVVEWQNRPLDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPDAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRD
                     LKAIYQAPTEEAGQQALEAFAAAWDSRYPQISRSWQANWPNLATFFAYPTDIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQSASQKWTMSLKDWR"
BASE COUNT          334 a          301 c          309 g          261 t
ORIGIN      
        1 taattgcctg attttgatat gttcaatcca gcatcaaatg aaggttaatt tatggacgaa
       61 aaacagttac aggctctggc taacgaactg gctaaaaacc tcaaaacccc tgaagacctc
      121 agtcagtttg atcggctgct gaaaaagctc agcgttgaag ccgctctcaa tgcagagatg
      181 acacaccatc ttgggtatga gaaaaatcag tccaggccag gagctaactc ccgcaacggt
      241 tattccacaa agaccgttat cacaggcgac ggtccactgg aactgcgtac tccgcgcgat
      301 cgtgacggta ccttcgaacc acaactggta aagaaaaatc agacccgtat taccgggatg
      361 gataaccaga tcctctcgtt gtatgccaaa gggatgacca cccgtgagat agctactgcg
      421 ttcaaagaac tgtatgacgc agatgtttca ccggcactga tatcaaaagt caccgatgcc
      481 gtgatggagc aggttgtaga atggcaaaac agaccactgg atgctgttta ccccattgtt
      541 tatcttgact gtatcgtcct gaaagttcgg caggacagtc gcgtcatcaa caaatcggtg
      601 ttcctggcac tgggcatcaa tatcgaaggt cagaaagaac tgctgggtat gtggctggcc
      661 gaaaatgaag gggcgaagtt ctggctcaat gtgctgactg aactgaaaaa ccgcggtctg
      721 aacgatatcc tcatcgcctg tgtggatggc ctgaaaggct tcccggatgc catcaacaca
      781 gtatatccga aggcccgcat ccagttatgc atcgtgcata tggtgcgcaa cagcctgcgc
      841 ttcgtgtcat ggaaggacta caaagccgtc actcgcgacc tgaaagcgat ttatcaggct
      901 cccacggaag aggcaggtca gcaggcactg gaagcgttcg ctgcggcctg ggacagtcgc
      961 tatcctcaga taagccgaag ctggcaggct aactggccga atcttgccac gttcttcgct
     1021 tatccaacgg acatccgcaa agtgatctat acgacgaatg ccatcgagtc gctaaacagc
     1081 gtgatccgcc atgcgatcaa aaagcgtaaa gtgttcccga cagacgactc ggtgaaaaaa
     1141 gtggtgtggc tggcaatcca gtctgcgtcc cagaaatgga cgatgtcgtt gaaggactgg
     1201 cgaat
//
LOCUS       DAZKFU020000558         1204 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZKFU020000558 DAZKFU020000000
VERSION     DAZKFU020000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.568"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..131)
                     /locus_tag="ACHOQQ_RS25580"
                     /old_locus_tag="pgaptmp_005116"
     CDS             complement(<1..131)
                     /locus_tag="ACHOQQ_RS25580"
                     /old_locus_tag="pgaptmp_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HGU4473221.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQK"
     gene            177..1034
                     /locus_tag="ACHOQQ_RS25585"
                     /old_locus_tag="pgaptmp_005117"
     CDS             177..1034
                     /locus_tag="ACHOQQ_RS25585"
                     /old_locus_tag="pgaptmp_005117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473222.1"
                     /translation="MRITDNKKTNQAEYTFNADYFLIAVDTGNTAHFLTSVATGSTVR
                     FLTSAATGSTVHFLTSAATGSTVRFLTSAATGSTVRFLTSAATGSTAHFLISAATGST
                     VHFLTSSATGSTVRFLTSAATGSTVHFLTSTATGSTAHFLIPAATGSAAHFLISAATG
                     STAHFLISAATGSTAHFLISAATGNTAHFLITVTTGSTVRFLTSSATGSAAHFLISAA
                     TGSTAHFLISAATGNTAHFLITVTTGSTAHFLTSVATGNTAHFLITVATGNMVNDDYY
                     LNVQRQKQH"
BASE COUNT          269 a          344 c          216 g          375 t
ORIGIN      
        1 tccttctgat tattcctgcg tccctcccag agacggatcg catatcgggt atgaagggca
       61 atagtcagcg atgactgcag agcaccaact tgttctgatt ttctgacttc tccggttttc
      121 tcctggggca ttgtgtttac tccaaatgat ttcatgacca gaagatagtc cgcaggttgc
      181 gtattaccga caacaaaaaa acaaatcagg cagaatatac atttaacgcc gattattttt
      241 taatcgctgt tgacactggc aacaccgctc actttttaac ctctgttgcc actggcagca
      301 ctgttcgctt tttaacctct gctgccactg gcagcactgt tcacttttta acctctgctg
      361 ccactggcag cactgttcgc tttttaacct ctgctgccac tggcagcact gttcgctttt
      421 taacctctgc tgccactggc agcactgctc attttttaat ctctgctgcc actggcagca
      481 ctgttcactt tttaacctct tctgccactg gcagcactgt tcgcttttta acctctgctg
      541 ccactggcag cactgttcac tttttgacct ctactgccac tggcagcact gctcattttt
      601 taatccctgc tgccactggc agcgctgctc attttttaat ctctgctgcc actggcagca
      661 ctgctcattt tttaatctct gctgccactg gcagtactgc tcatttttta atctctgctg
      721 ccactggcaa caccgctcat tttttaatca ccgttaccac tggcagcact gttcgctttt
      781 taacctcttc tgccactggc agcgctgctc attttttaat ctctgctgcc actggcagca
      841 ctgctcattt tttaatctct gctgccactg gcaacaccgc tcatttttta atcaccgtta
      901 ccactggcag cactgctcat tttttaacct ctgttgccac tggcaacacc gctcattttt
      961 taatcactgt tgccactggc aacatggtta atgatgacta ctaccttaac gttcagagac
     1021 aaaaacagca ttaatgtcgt tatccccatc acgagcaact gaatgtcggt tatatccaga
     1081 tgagacaatg accagaaaat cacaaaaaat gaaaagcatt ctgccagcag actcaggagg
     1141 aacataaatt gcaggccccc tccgactgta ctgcctgtac gcgctttata aagactgtat
     1201 gtga
//
LOCUS       DAZKFU020000559         1203 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZKFU020000559 DAZKFU020000000
VERSION     DAZKFU020000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.569"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..1173
                     /locus_tag="ACHOQQ_RS25590"
                     /old_locus_tag="pgaptmp_005118"
     CDS             31..1173
                     /locus_tag="ACHOQQ_RS25590"
                     /old_locus_tag="pgaptmp_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HGU4473223.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT          363 a          256 c          266 g          318 t
ORIGIN      
        1 caaaatcaga taaacatttg aggtattgtt atgacaaata aacacccatc attgttttct
       61 ccttttatgc tgacagaaaa aataaagcta cgtaatcgta tcgttatggc tcctatgacg
      121 acctggtcag cgaatcctga tggaaccatc tctgagcaag aactcgaatt ttataaacgt
      181 cgctcacaaa atgttggttt agtaataaca ggatgtacct atgtcacgcc aagcggcatt
      241 gggtttactc atgaatttgc cgcgtatgac gaccgtttca ttaacagcct tgaaaagtta
      301 gcagcagcag ccaaaagtgg gggtgctccg gctattttgc agattttcca cgccggtaat
      361 aaagccatcc cggagcttgt accaaataac gatgttatta gtgccagcgc atcaagcgta
      421 aaatctggtg actttatgaa gagagtggta caaagcaggg aaatgactga aaacgaaatt
      481 caggaaacca ttagagcatt cggagatgtc acaaaaaggg cgattaaagc aggttttgat
      541 ggcattgaac ttcatggtgc gcacggattc cttctacaga atttcttctc ccctctattt
      601 aaccagcgta atgaccgatg gggaggagat ctcgaaggac gtatgcgctt cccgctggcc
      661 gtattacagg aagtaaaaaa tgtagtctat gaatatgcga caaagccctt tgccatcggt
      721 tacagaatat ctcctgaaga atctgcaaca ggggggctgc ggatagagga tacttataaa
      781 ctccttgatc ggctcatttc atccggtatt tcgtatatac atacctcgct ggtcagtatt
      841 aacgatagct accctgttga gtcgccaaat ggcccccgaa ctattgaatt gattttaaat
      901 catattgcag gcagagtgcc ggtaatcgct gcaggtaaaa tacgcacacc gtctcaggct
      961 caagaagcaa tttccactgg cctgccactt gttgcgatcg gtaaagggct ggtgataaat
     1021 ccagaatggg tgactttagc tgaatcaggt agaggccatg aaattcaaac aacccttaat
     1081 ccacaacttg ttcctgaatt aaccattccg gataaactat gggatcaaat tcaggcatcg
     1141 aaaggaactg gctggtttcc gttaatggat taaatatcta ttactctccc gagagtaaaa
     1201 cta
//
LOCUS       DAZKFU020000560         1195 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZKFU020000560 DAZKFU020000000
VERSION     DAZKFU020000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.570"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..720
                     /gene="ais"
                     /locus_tag="ACHOQQ_RS25595"
                     /old_locus_tag="pgaptmp_005119"
     CDS             118..720
                     /gene="ais"
                     /locus_tag="ACHOQQ_RS25595"
                     /old_locus_tag="pgaptmp_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGU4473224.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHOQQ_RS25600"
                     /old_locus_tag="pgaptmp_005120"
     CDS             complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHOQQ_RS25600"
                     /old_locus_tag="pgaptmp_005120"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGU4473225.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEIKPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          318 a          277 c          257 g          343 t
ORIGIN      
        1 ggcattttgc cgacatcgta ggatttttaa tattacacca actgctaatt atcgccagaa
       61 atgtcgctca atttcacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
      121 ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt actggcgctc
      181 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      241 gacaataaaa cactggccag actggcacag cagcacccgg ttgtcgtttt gtttcgccat
      301 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtc
      361 aaaggtaccc aggatgcccg agaactgggc aacgctttta gtgctgatat ccctgatttc
      421 gatctctatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt ttcagcgggt
      481 aaaaaattga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcaatt
      541 aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca taatcattgc
      601 ctgacatata ttgctaaaga taagcgtgat gcgacattta aacctgatta tctggatggt
      661 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taaccactaa
      721 attttcaatc tgacagccag tgatggctgt catcattgtt aaagaagacc tttcaaacgt
      781 aacgtttttc gggtggcaac attcaaatca taatgcacca gatcttcagg ttttacccac
      841 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcagtcaaaa
      901 atcaggtaaa tcatataaat ctcttccttg cgaccatctg catacgtctt ggtgcgaata
      961 tcatcgctga aggtccacgg cttgatttct gtcaaaagca gctgttctcc cagttcttcg
     1021 cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccgg gctccacgcc gccacccgaa
     1081 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag caaataagca
     1141 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcacaggggt attcc
//
LOCUS       DAZKFU020000561         1194 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZKFU020000561 DAZKFU020000000
VERSION     DAZKFU020000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.571"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(78..1022)
                     /gene="leuO"
                     /locus_tag="ACHOQQ_RS25605"
                     /old_locus_tag="pgaptmp_005121"
     CDS             complement(78..1022)
                     /gene="leuO"
                     /locus_tag="ACHOQQ_RS25605"
                     /old_locus_tag="pgaptmp_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGU4473226.1"
                     /translation="MPEVQTDHPEMAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          364 a          272 c          241 g          317 t
ORIGIN      
        1 attcagaata aaattctaca ttcaataaaa aatgcagaat aaaccagaca ttcatgtctg
       61 acctattctg cattcagtta gcgtttgcaa attgagacta attgctcttc catccactga
      121 tggcctttat cgcgcccggc agcttcatgc caggagagat aacaggttct gctatttagt
      181 tttaacggca gcggtaatac ctgtaattct aaggattcag cgaactcttc agccagccaa
      241 cgcggcgcaa tagcgaccaa atgcgtttgc gacaccacgc taagtacgct catcattgcc
      301 atgccctgat acgcgatact ggcttgctta tctaccgtgt cataccacgg ttgactaaat
      361 gacgcgaaac gatcgagcga aaccgccgca tgttgttcgt tataaacatc atgtttcagt
      421 aacgggccct taattgttgg gtgatttttg ctggctacca gcaccatttc atctttaaat
      481 aatggcacgc tggtaaattc aggacgatgg aagtcttcat aactaatcac aaactccgtt
      541 tcctgataac gcagctgatg ttcagtgttc tgatttaatg aagacttgaa cataacatgt
      601 atatttggcg caatctgctc aatgtgatta taaatctgcg aggtcagaat actatctaac
      661 gggctgcaaa cacaaagatg aaatacacgt tcactgctcg cgggttcaaa acctgaacca
      721 ggcaattcat tttgtactag ttgcaatgcc tgacgaactg aaccaaaaag ttgaaatgcg
      781 cgagcagtcg gttgaatacc acggccataa cgaacaaaaa gctcgtcatt aaacatcacc
      841 ttcaggcgtg caacagcgtt actgaccgca ggttgcgaca ttcccagaac atgagcagca
      901 cgggtaatgt tttgctcctg catcacggca tcgaaaacgg ttaataagtt gagatcgacc
      961 atgcgtagct gtggcttgct gaactccgcc atctctggat gatctgtttg tacctctggc
     1021 atacttaact ccactgtcac acttaactcc ctttccctta ttggaatgca gagaaataat
     1081 cctcgagaat atgatttatc atgcatataa aataagaaaa aggaaaatga gtaaaattag
     1141 gaaaacataa agatacgggt tcttataaaa acagaatcca ttgccgtgga aagc
//
LOCUS       DAZKFU020000562         1190 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZKFU020000562 DAZKFU020000000
VERSION     DAZKFU020000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.572"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..1099
                     /locus_tag="ACHOQQ_RS25610"
                     /old_locus_tag="pgaptmp_005122"
     CDS             2..1099
                     /locus_tag="ACHOQQ_RS25610"
                     /old_locus_tag="pgaptmp_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4473227.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
BASE COUNT          331 a          263 c          269 g          327 t
ORIGIN      
        1 gatgaaaaaa ttaacagtgg caatttctgc tgtagctgca tcagtactga tggcgatgtc
       61 tgctcaggca gctgaaattt ataataaaga cagtaacaag ctggatctgt acgggaaagt
      121 taatgccaag cactactttt cctctaacga tgcagatgat ggtgatacta cttatgttcg
      181 tctgggcttc aaaggcgaaa cccaaatcaa cgatcagctg actggtttcg gtcagtggga
      241 atatgaattc aaaggcaacc gcgctgaatc tcaaggttcc tccaaagaca aaacccgtct
      301 tgcatttgca ggcctgaaat tcggtgacta cggctcaatc gattacggcc gtaactacgg
      361 tgtagcatac gacatcggtg cgtggactga cgttctgcca gaattcggtg gcgatacctg
      421 gacccaaaca gatgtgttca tgactggtcg caccactggt gttgcaacct atcgtaacaa
      481 cgacttcttt ggtctggttg atggtctgaa ctttgctgct cagtaccaag gcaaaaacga
      541 tcgtagcgat ttcgataact acaccgaagg taacggtgat ggcttcggtt tctctgctac
      601 ctatgaatac gaagggttcg gtatcggtgc aacttatgcg aaatctgatc gtaccgacac
      661 tcaagttaat gcagggaaag ttcttcctga agtatttgct tccggtaaaa atgcagaagt
      721 ttgggccgca ggtctgaaat atgacgctaa caacatttac ctggccacta cctattctga
      781 aacccagaat atgactgtat ttgctgatca cttcgttgct aataaagctc aaaacttcga
      841 agctgttgca caatatcagt tcgatttcgg tctgcgtccg tccgttgctt acctgcaatc
      901 taaaggtaag gatcttggag tatggggcga tcaggactta gtcaaatatg ttgatgtagg
      961 tgcaacctat tacttcaaca aaaatatgtc tactttcgtt gattacaaaa tcaacctgct
     1021 tgacaaaaat gacttcacta aagcactcgg tgtaagcact gatgacatcg ttgctgtagg
     1081 tctggtttac cagttctaat ctgattacga aaaagatatg ttgcgggagg ctttgcctcc
     1141 ccaacatata agtggctccc tcaagccact tcctttagga gcacaacctt
//
LOCUS       DAZKFU020000563         1179 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZKFU020000563 DAZKFU020000000
VERSION     DAZKFU020000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.573"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..713
                     /locus_tag="ACHOQQ_RS25615"
                     /old_locus_tag="pgaptmp_005123"
     CDS             <1..713
                     /locus_tag="ACHOQQ_RS25615"
                     /old_locus_tag="pgaptmp_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4473228.1"
                     /translation="TAWYAEYDEWGNQLNEENPHQLQQLIRLPGQQYDEESGLYYNRH
                     RYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNK
                     PPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTC
                     TPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDD
                     LGEILAKMKQRTSRGIRK"
     gene            710..>1179
                     /gene="ybfC"
                     /locus_tag="ACHOQQ_RS25620"
                     /old_locus_tag="pgaptmp_005124"
     CDS             710..>1179
                     /gene="ybfC"
                     /locus_tag="ACHOQQ_RS25620"
                     /old_locus_tag="pgaptmp_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HGU4473229.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWKWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKP"
BASE COUNT          381 a          234 c          270 g          294 t
ORIGIN      
        1 caacagcgtg gtacgcagaa tacgatgaat ggggtaacca gctgaatgaa gagaacccgc
       61 accagctgca gcagctcatc cgcctgccgg gtcagcagta tgatgaggag tccggcctgt
      121 attacaaccg ccaccgctat tatgacccgc tgcaggggag gtatatcact caggatccga
      181 ttggactgaa ggggggatgg aatttttatc agtatccgtt gaatccggtt cagtatatag
      241 attcaatggg actggcatca aaatatggac acttaaataa tggcggatat ggagcgagac
      301 ccaacaaacc gcctacgccc gatccaagta aattgccaga catagcgaaa caattaagac
      361 tgccatatcc tattgaccag gccagtagtg cgcctaatgt tttcaaaaca ttcttcagag
      421 cattaagccc ttacgactac acactgtatt gcaggaagtg ggtaaaacca aatctgactt
      481 gtacgccaca ggatgatccc cagtatccag ggatggatac aaagacagca agtgattacc
      541 tgccacagac aaattggcca acaactcaat taccaccagg atatacttgt gcagaaccct
      601 atttattccc agacattaat aaacccgatg ggccagcaac agcagggata gatgatttgg
      661 gtgaaatttt agctaaaatg aaacagagaa catcgagagg aataagaaaa tgaaaagagt
      721 tttgttcttt ttgctgatga tatttgttag ttttggtgtt atagctgatt gcgaaataca
      781 agctaaagat catgattgtt ttactatttt cgctaagggt actatttttt ccgcgtttcc
      841 tgtcttaaat aataaagcca tgtggaaatg gtatcagaat gaggacatcg gtgagtatta
      901 ttggcaaaca gaactgggta catgtaaaaa taataaattc acaccaagcg gggcgagact
      961 cttaattcgc gtcgggtcac tacgccttaa tgaaaatcat gccatcaaag gaacgttgca
     1021 ggagttgata aatacagcag aaaaaacggc gtttctcggc gatcggttta gaagttatat
     1081 aagagcgggt atctatcaaa aaaagagcag tgatccagct cagttgttgg cagtccttga
     1141 taattcaatc atggttaaat attttaaaga tgagaagcc
//
LOCUS       DAZKFU020000564         1173 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZKFU020000564 DAZKFU020000000
VERSION     DAZKFU020000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.574"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(11..>1173)
                     /locus_tag="ACHOQQ_RS25625"
                     /old_locus_tag="pgaptmp_005125"
     CDS             complement(11..>1173)
                     /locus_tag="ACHOQQ_RS25625"
                     /old_locus_tag="pgaptmp_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4473230.1"
                     /translation="STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNG
                     VFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFN
                     SLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFL
                     AYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYAD
                     TAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKS
                     LWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASL
                     PILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT          340 a          335 c          292 g          206 t
ORIGIN      
        1 cagagaggtg ttatacggtt ttattgcgct tcatgaccat tgccacaata aggctgagta
       61 agattgcacc aataatccat atcaacgcac ggctgccctg ggaccacagg ctgtagataa
      121 agccaacgat caccaacaac atcaccaacg tacctacgat aagaatcggc aacgaggcgt
      181 gaatatcatg acgcaggcgg attgccacca caaacaccgc cagatagcaa atcaaaaagg
      241 tggcgctggc aacgctggcg agtgagccta aatttagtgc cgccgtcatc agcataatca
      301 acaccacgac aatgatgttg ccccaggtac tctgccgcca cagggattta ttcattagct
      361 tcggcagttc gcgttcgctg cccatgttgt ccatgatgtt aaacacggcg aacaggttcg
      421 cgttgatggc tgaagccgtc gccagtaaag cgccgatgac gacgatcaca taacccacat
      481 gcccgagcag cggagaagct gcctgcgcta cggcggtatc ggcatatttt tctaactcta
      541 atgccgatac atcgctaagc aaaaccagcg ccagcgagat ataaagcaac gtggtaacgc
      601 caattgccac cagaaacgcc cgtggcataa tgacctgcgg atctttcact ttatccgccg
      661 cgttcgccat catgccaaag cccgcatagg caaggaaggt tatcccgata caggagaaga
      721 acgcaccgga gctggggggc gcagagacgg aaatatgcgc cggttgcagc gaccagacac
      781 cggcaataat cagcaataac aggatcatca ttttaatgcc gacgaggatc acctccagcc
      841 gccctaccgc atggttgctt aaggagttga aaagcgtcat caccgcaatg atccccaacg
      901 cgtagagcaa aataaggtgc tcctcctggc tgccttcatg caaaaactgc acggcataag
      961 cgccaaaagc acgggcgacc atggcgatgc tcaccgccag cgtcaacagg tacagtaacg
     1021 agagcgccag cgaaaagacg ccgttgccta atccgcgacg aaagaagtcg ataatgccgc
     1081 cattgctggg atagctcgcc cccagacgcg cataggcata accggaaaac atcgccacaa
     1141 taccgccaaa agcaaaggcg acccaggtcg agg
//
LOCUS       DAZKFU020000565         1171 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZKFU020000565 DAZKFU020000000
VERSION     DAZKFU020000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.575"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(107..>1171)
                     /locus_tag="ACHOQQ_RS25630"
                     /old_locus_tag="pgaptmp_005126"
     CDS             complement(107..>1171)
                     /locus_tag="ACHOQQ_RS25630"
                     /old_locus_tag="pgaptmp_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4473231.1"
                     /translation="LLAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGDKTY
                     ARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGSLDYG
                     RNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDFFGLIEGLNFAAQ
                     YQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAAN
                     PLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQF
                     DFGLRPSLAYLKSKGKDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDF
                     TRAVDIRTDNIVATGITYQF"
BASE COUNT          325 a          265 c          257 g          324 t
ORIGIN      
        1 ggtgaatgac cagaaatatc aaaaattgct catttttact taaggagtca tacagcagca
       61 cttaaatcaa aaaccagccc agagaagggc tggtcaggag aggatgttag aactgatagg
      121 taatgcccgt agcgacgatg ttatcggtgc gaatatctac ggcacgggta aagtcgcttt
      181 catcaatcag gttgatttta taatcaacat aggtcgacat atttttgttg aagaaatacg
      241 tcgcaccaac gtcgatatac tcaatcatgt cctggtcgcc gtagcggccc agatctttgc
      301 ctttcgattt caggtaagcg agtgatgggc gcaagccgaa gtcgaattga tattgcgcca
      361 ccacttcaaa actttgtgct ttgttagaga tataaccatc cccataggtg gtcatgttta
      421 atgtttcagc gtaattagct gcaaagtaga tgttgttggc atcatacttt atacctgtgg
      481 cccacagttc tgcattctta ccggaggcat tgagaggatt agcagcggct ctttcctgcg
      541 catttgtccg atcggatttg gtatataccg caccgatacc aaagccatca taaacataag
      601 ttgaggagat accgaaaccg tcaccgttgg cacgcgtgta gtcagcacca taaagatgac
      661 tgttgtcagt acgttcattt ttgccttgat attgcgcggc aaagttcagc ccttcaatta
      721 agccaaagaa atcgtagttg cgataggttg ctacaccggt ggtacggtat gtcatgaaga
      781 catctttctg actccaggta tcgccaccaa attctggcaa catatcagtc catgaaccaa
      841 tgtcatagcc gacaccgtag ttacggccat agtccagaga gccaaattca ctaattttta
      901 aaccagcata ggccagacgg gtcttgttgc cgcgcgagcc gttggcttca tcgttatagc
      961 ctttaaaatc atattcccag tgaccaaaac caatcatttg atcattgatt tgcgtttctc
     1021 ctttaaagcc gagacgggca taagttttat caccatcgtc acgcttatca tcagtaaaat
     1081 aacgtagagc ggtaaccttg ccataaaggt cgagtttatt accatccttg ttatagactt
     1141 cggcagcgtg tgctacgttc gcagctaaca a
//
LOCUS       DAZKFU020000566         1167 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZKFU020000566 DAZKFU020000000
VERSION     DAZKFU020000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.576"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..85
                     /locus_tag="ACHOQQ_RS25635"
                     /old_locus_tag="pgaptmp_005127"
     CDS             <1..85
                     /locus_tag="ACHOQQ_RS25635"
                     /old_locus_tag="pgaptmp_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HGU4473232.1"
                     /translation="DYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            186..1064
                     /gene="glxR"
                     /locus_tag="ACHOQQ_RS25640"
                     /old_locus_tag="pgaptmp_005128"
     CDS             186..1064
                     /gene="glxR"
                     /locus_tag="ACHOQQ_RS25640"
                     /old_locus_tag="pgaptmp_005128"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4473233.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
BASE COUNT          313 a          255 c          305 g          294 t
ORIGIN      
        1 tgactacaac ggttgggttg ggtgtgaata taaaccccaa accaccacgg aagccggttt
       61 acgctggatg gatccgtacc gttaaaacgt aacgctattc agacaatgct tttttaggcc
      121 gctaagttgg caggggatcg tgttgtctga attcaggaat agcgacattt aaaaagaggt
      181 taattatgaa actgggattt attggcttag gcattatggg tactccgatg gccattaatc
      241 tggcgcgtgc cggtcatcaa ttacatgtca cgaccattgg accggttgct gatgaattac
      301 tgtcactcgg tgctgttaat gtcgacaccg cgcgccaggt aacggaagca gcggacatca
      361 tttttattat ggtgccggac acacctcagg ttgaagaagt tctgtttggt gaaaatggtt
      421 gtaccaaagc ctcgctgaag ggcaaaacca ttgttgatat gagctccatt tcaccgattg
      481 aaactaaacg tttcgctcgt caggtgaatg aactgggtgg tgattatctc gatgcgccag
      541 tctccggcgg cgaaatcggc gcgcgtgaag ggacgttgtc aattatggtt ggcggtgacg
      601 aagcggtatt tgaacgtgtt aaaccgctgt ttgaactgct cggtaaaaat atcacccttg
      661 tgggcggtaa cggcgacggt caaacctgca aagtggcaaa tcagattatc gtagcgctca
      721 atattgaagc ggtctctgaa gctctgctgt ttgcttcaaa agccggtgca gacccggtac
      781 gtgtgcgcca ggcgctgatg ggcggctttg cttcctcacg tattctggaa gttcacggtg
      841 agcgcatgat taaacgcacc tttaatccag gtttcaaaat tgcgctgcac cagaaagatc
      901 tcaacctggc actgcaaagt gcgaaggcac tggcgctgaa cctgccaaac actgcgacct
      961 gccaggagtt atttaatacc tgcgcggcaa acggtggcag ccagttggat cactctgcgt
     1021 tagtgcaggc gctggaatta atggctaacc ataaactggc ctgatacccg caataaaaat
     1081 ggccgatatc aaaaaatgaa tcggccagca atattaaaaa agaaagcagc caaagatgtt
     1141 gcttcagtat taaaaataat attttta
//
LOCUS       DAZKFU020000567         1160 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZKFU020000567 DAZKFU020000000
VERSION     DAZKFU020000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.577"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..408
                     /locus_tag="ACHOQQ_RS25645"
                     /old_locus_tag="pgaptmp_005129"
     CDS             151..408
                     /locus_tag="ACHOQQ_RS25645"
                     /old_locus_tag="pgaptmp_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HGU4473234.1"
                     /translation="MLHIIRKGDKTTHGGSVLTASETMKFGGIGVARKGDKVSCPGHG
                     PTIIIEGNPDYLDHGIPVAFHGHKCACGCTLISSFAAAKVA"
     gene            411..>1160
                     /locus_tag="ACHOQQ_RS25650"
                     /old_locus_tag="pgaptmp_005130"
     CDS             411..>1160
                     /locus_tag="ACHOQQ_RS25650"
                     /old_locus_tag="pgaptmp_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004170999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473235.1"
                     /translation="MPVWLDAIPEKAPKIARPNTRRWLLFLAFIMLGGMALTLWCWTS
                     ERTGFVFWFTALGLPFCTWGLIFGLRRFAYKAGQVGAESRNVEREALIDSEILRGQRC
                     AWILGTYIQSPAGNKADDLLEAMEVAAPAIDFSHPRGCDKPVRYAALPEYQTDLTKAL
                     KGAVNKLTTRVEGIVHPLPPALPCWLVLDCDNDLYPLIEEQLKADLSLKTGRIFRLMT
                     GKGLGAFDAWLDKRWDTPGILVVITLSLPASP"
BASE COUNT          283 a          273 c          317 g          287 t
ORIGIN      
        1 gcgatggaga actctatcta tcaataatca gtgacccgga aacattcata ataaaaatcc
       61 agacaatcac tatcgatggc agagcccagc ataaacatgg gaattaaaga gtgatgaatt
      121 agttaagaga taagaaagag gaatttaata atgcttcata ttattcgtaa aggcgataaa
      181 actactcacg gaggttccgt tctgaccgct tctgaaacga tgaaattcgg tgggataggc
      241 gtcgcccgta aaggcgataa agtgtcatgt ccgggtcatg gtcctaccat catcatagaa
      301 ggcaatcctg attatcttga ccatggtatc ccggtggcat ttcacggtca taaatgcgcc
      361 tgtggttgca cattgatcag ttcatttgcc gcagcaaagg ttgcctgatt atgcctgtct
      421 ggctggatgc catccctgaa aaggccccaa agatagcgcg ccccaatact cgccgctggc
      481 tgttgttcct ggcatttatc atgctggggg ggatggcgct cacattatgg tgctggacgt
      541 cagaacgaac aggatttgtc ttctggttca ccgctctggg gcttccattt tgcacgtggg
      601 ggcttatttt cggtttacgt cggtttgcct ataaagccgg gcaggtgggg gctgagtccc
      661 gaaatgtcga gcgggaagcg cttattgaca gtgaaattct gcgtggtcag cgctgtgcct
      721 ggatcctcgg tacttatatt cagagcccgg ccggaaacaa agccgatgac ctgcttgagg
      781 caatggaagt cgcggctcct gctattgatt tctcgcaccc aagaggatgt gataaaccgg
      841 tcaggtacgc ggcattacca gaataccaga cagatttaac gaaggctctg aaaggggcgg
      901 tgaataaact gaccacgcgt gtagagggga tagttcatcc gcttccgccc gcgctaccat
      961 gctggctggt gctggattgt gataacgatc tgtatcccct gattgaagag cagcttaaag
     1021 ccgacctctc actcaagacg ggcaggattt ttcgcctgat gaccggaaaa gggctggggg
     1081 cgtttgatgc ctggctggac aagcgatggg acaccccggg cattctggtt gtcatcacgc
     1141 tgtcgttacc tgcctctccc
//
LOCUS       DAZKFU020000568         1156 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZKFU020000568 DAZKFU020000000
VERSION     DAZKFU020000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.578"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..932
                     /locus_tag="ACHOQQ_RS25655"
                     /old_locus_tag="pgaptmp_005131"
     CDS             <1..932
                     /locus_tag="ACHOQQ_RS25655"
                     /old_locus_tag="pgaptmp_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGU4473236.1"
                     /translation="MAIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVT
                     MTMQDHATLKEIKDLAKNITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGL
                     TMKCYSPVCKNASRMDQNLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREE
                     MRSLGSYIALNLEGSSQERTFSLLIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVD
                     CYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPA
                     MADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT          370 a          218 c          253 g          315 t
ORIGIN      
        1 ctatggccat ctttcctgtt attcgggcgc ttgaattgca taaaattaag catctactaa
       61 ttgttacgtc gacaattaat ttagaagtct ttaatgcgct caaacttgaa cagactaaat
      121 taacattagt cacaatgact atgcaggatc acgcaacatt aaaagaaata aaagatttag
      181 cgaagaacat aacacagcaa tacggtacgc cagatctttg tatagaggcg atgcgtaaaa
      241 agaacctgaa aacgatgtta tttatcagtc agttgaaagc aaaaacgaat tttcaggttg
      301 ttggtttaac catgaagtgc tattcccctg tgtgtaagaa cgcgtcccgg atggatcaga
      361 atctccgggc tcccgtaccc atgacatggg catttatgat gcgtgaggcg ggttttccag
      421 cagtcaggcc aatatatgaa ttgccactaa gtgaggatgt actcgatgag gtgcgcgagg
      481 aaatgcgatc gttaggatct tacattgcgc ttaatttaga aggtagctcg caggaacgta
      541 cattttcatt attgattgca gaaaatctaa tagcaaaaat tcaaagtgaa acagatatac
      601 caatagtgat cgttcatgga ccaaaagggg aagataaagc cagggtatta gtggattgtt
      661 ataataatgt ctaccgttta tccttatcac cctcgattaa acgttcagca gcaatcataa
      721 aagatgctta tatcgcaata actcctgaca cctcaatatt acatatggca agtgcctata
      781 atacccccgt tgttgcaatt tatgctgatt acaaaacgcg atggcccgca atggccgatg
      841 tttcggagtc agtcgtcgtt gggcaaaaaa ttgacaatat aagtctggat gaattcgcaa
      901 aggcattaaa aagtgttttg gcgagaattt gaggcagtac gtatgcatag taatgccgat
      961 ctgttatgaa cggtggtgtc atcaatactc tgaggctggt caatcatcac agaaaaccag
     1021 cctttggatc aaagctgtac tcaccggata cgatcatagt cacaccgaac gcgaatctgg
     1081 cggtatcgca tttttattta ttcattttcg gtgagagaag agcaagctct atagagggaa
     1141 tgtaaaaaag agtgcc
//
LOCUS       DAZKFU020000569         1154 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZKFU020000569 DAZKFU020000000
VERSION     DAZKFU020000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.579"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..729
                     /locus_tag="ACHOQQ_RS25660"
                     /old_locus_tag="pgaptmp_005132"
     CDS             <1..729
                     /locus_tag="ACHOQQ_RS25660"
                     /old_locus_tag="pgaptmp_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4473237.1"
                     /translation="LNDPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEG
                     EQFDTVEVDFGRSEGNNIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDG
                     TVWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAE
                     DGTEKRYLPEGTLVLGNTSAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPARE
                     FTMTQSAPLMVLPDPDEFVVVQVK"
     gene            781..>1154
                     /gene="gpFI"
                     /locus_tag="ACHOQQ_RS25665"
                     /old_locus_tag="pgaptmp_005133"
     CDS             781..>1154
                     /gene="gpFI"
                     /locus_tag="ACHOQQ_RS25665"
                     /old_locus_tag="pgaptmp_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4473238.1"
                     /translation="MATKEENLNRLRQLAGLLGREVDMSGSAADIALRVSEWEEELAA
                     SREGIMHSDESGSDQNDTDDGEQLNNTDSPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTA"
BASE COUNT          262 a          258 c          375 g          259 t
ORIGIN      
        1 ctgaacgacc cggcctaccg tcgtctgcgt atcatcactg ataacctcaa acaggaagag
       61 catgccattg tccaggtgga ggaaatgcag gcggtgaatg ccgtgctgta tggcaaatac
      121 accatggaag gggagcagtt tgatactgtc gaggtggatt tcgggcgctc tgaaggaaat
      181 aacattgagc aggctgacgg taaaaaatgg tctgagcagg accgtgatac gtttgatccg
      241 acgcatgata ttgacctcta ctgcgatcag gccagcggtc ttgtgaatat cgccattatg
      301 gacggtacgg tctggcgtct gctgaatggc tttaagttgt tccgcgaaaa actggatacc
      361 cgtcgcggct caaattcaca actcgaaacg gcagtgaaag atctgggcgc agtggtgtcc
      421 ttcaaggggt attacggcga tctggccatt gtggtggcga aaacgtctta tgtggcagag
      481 gacggtaccg aaaaacgtta tctgccggag ggcacgctgg tcctgggaaa tacgtcagcg
      541 gagggcatcc gttgctatgg tgccattcag gatgcgcagg cgttgtccga aggtgtggtg
      601 gcctcttccc gttatccgaa acactggatg accgtgggcg atccggcccg tgaattcacc
      661 atgacgcagt ccgcgccgct gatggtgctg ccggatccgg atgagtttgt ggtggtacag
      721 gtgaaataat ccgggagcgg gggcgaaatg cccccgtgtc ttttttcaca gggggctgag
      781 atggcaacaa aagaagaaaa tctgaatcgt cttcgtcaac tggctggtct gctggggcgc
      841 gaggtggata tgtcggggag tgctgcggat attgctctgc gtgtgtctga gtgggaagag
      901 gagcttgcag cttcccggga gggcattatg cactctgatg agagcgggtc tgatcaaaat
      961 gacacagacg atggtgagca gttgaacaac acggattctc cggatgatgt taaagccgtc
     1021 cgggtacgga agtgcctgca agtaatgggg tattgcccgg agacaggtcg tcccgttgag
     1081 ctggcgttac ggggtatgcg tgttctggtg ccatcatcac tggcaacggc catgatacag
     1141 cacggaacgg ctga
//
LOCUS       DAZKFU020000570         1153 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZKFU020000570 DAZKFU020000000
VERSION     DAZKFU020000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.580"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..1028
                     /locus_tag="ACHOQQ_RS25670"
                     /old_locus_tag="pgaptmp_005134"
     CDS             48..1028
                     /locus_tag="ACHOQQ_RS25670"
                     /old_locus_tag="pgaptmp_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HGU4473239.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRARVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
BASE COUNT          321 a          304 c          307 g          221 t
ORIGIN      
        1 gatatgagat catgtttgtc atctggagcc atagaacagg gttcatcatg agtcatcaac
       61 ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca gaccagaaaa gagattttct
      121 tgtcccgcat ggagcagatt ctgccatggc aaaacatggt ggaagtcatc gagccgtttt
      181 accccaaggc tggtaatggc cggcgacctt atccgctgga aaccatgcta cgcattcact
      241 gcatgcagca ttggtacaac ctgagcgatg gcgcgatgga agatgctctg tacgaaatcg
      301 cctccatgcg tctgtttgcc cggttatccc tggatagcgc cttgccggac cgcaccacca
      361 tcatgaattt ccgccacctg ctggagcagc atcaactggc ccgccaattg ttcaagacca
      421 tcaatcgctg gctggccgaa gcaggcgtca tgatgactca aggcaccttg gtcgatgcca
      481 ccatcattga ggcacccagc tcgaccaaga acaaagagca gcaacgcgat ccggagatgc
      541 atcagaccaa gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg
      601 ccaagagtgg cctgacccac agcctggtca ccaccgcggc caacgagcat gacctcaatc
      661 agctgggtaa tctgctgcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag
      721 gggcgccaca gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgcc
      781 ccggcaaggt aagaaccttg aaacagcatc cacgcaagaa caaaacggcc atcaacatcg
      841 aatacatgaa agccagcatc cgggccaggg tggagcaccc atttcgcatc atcaagcgac
      901 agttcggctt cgtgaaagcc agatacaagg ggttgctgaa aaacgataac caactggcga
      961 tgttattcac gctggccaac ctgtttcggg cggaccaaat gatacgtcag tgggagagat
     1021 ctcactaaaa actggggata acgccttaaa tggcgaagaa acggtctaaa taggctgatt
     1081 caaggcattt acgggagaaa aaatcggctc aaacatgaag aaatgaaatg actgagtcag
     1141 ccgagaagaa ttt
//
LOCUS       DAZKFU020000571         1145 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZKFU020000571 DAZKFU020000000
VERSION     DAZKFU020000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.581"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..652
                     /locus_tag="ACHOQQ_RS25675"
                     /old_locus_tag="pgaptmp_005135"
     CDS             <1..652
                     /locus_tag="ACHOQQ_RS25675"
                     /old_locus_tag="pgaptmp_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HGU4473240.1"
                     /translation="FDAVTAAMASAAPALGAMAVVAGPMGYDLLANDGRAGKALSDGQ
                     LINSQAEMCYWTSSCPLDSPHENDNSSGSQPNVGKDLTDEEKNCLAGGSTGSPGGWGP
                     EDENSARDRMQHERYKDELRQKMGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRY
                     ELATGEKVGGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            652..1029
                     /locus_tag="ACHOQQ_RS25680"
                     /old_locus_tag="pgaptmp_005136"
     CDS             652..1029
                     /locus_tag="ACHOQQ_RS25680"
                     /old_locus_tag="pgaptmp_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473241.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DVSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          354 a          181 c          311 g          299 t
ORIGIN      
        1 ttttgatgca gtcacggcgg ctatggcgtc tgctgcccct gctcttggag caatggctgt
       61 ggtcgctggg ccaatgggat atgaccttct cgctaatgat gggcgtgccg ggaaggctct
      121 gagcgatggg cagttaataa attctcaggc tgaaatgtgt tactggacca gtagttgtcc
      181 tttagatagc ccacatgaga atgataattc gtcagggagt cagccgaatg ttgggaagga
      241 tttgacggat gaagagaaga actgcctggc cggcggcagt acgggaagcc cgggaggatg
      301 gggaccagaa gatgagaata gcgcaaggga tcgtatgcag catgaacggt ataaagatga
      361 attgcgacag aaaatgggaa aacccgacgt tacagattca aatttgaaaa atattataga
      421 cgatctttat cggccaaatg caaaagtagg aagtggaagc acagcagatg ctgttcgtta
      481 tgaattggcc acaggtgaaa aagttggtgg tagaggacat gtattgaaag cacaggatta
      541 tacaagggct ttacaggatt ggttggataa gaatccaaca gcatctccag gggatcgagc
      601 agcagcagaa aacgttcttc aggatttaca gaatgctttg ggaggcaagt aatgaaccaa
      661 tatattgcta gttatataga aaaaatggca tcattttcca ctgttttgtc tgagacagtc
      721 tcattagcta atgaatactg gcatccagaa acgccaccgg tggttatgtt gttctctcag
      781 gttggaaaaa cgattgtaaa aaatttttat gaaatggggg atattgaaag agaaaaaata
      841 tttaaacatg tcgaagatgg tatggtctcg cttgatgatg aattggccac agttgtagct
      901 acagggctta tagaggcaat agtcactgcg accgatagca attctttact ttggcaagat
      961 gtaagtaaat ttctgggtga caaatcaaag tctcatgcat tagtgtggaa aaattttggt
     1021 caggagtgat attattcaag aagtattaag ctcgctattg agatcttaat gcttcttgag
     1081 attaaattaa agttaggacg aagtaattgc aggtggaaat aaatccatgg tgacgtgtgg
     1141 caatg
//
LOCUS       DAZKFU020000572         1142 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZKFU020000572 DAZKFU020000000
VERSION     DAZKFU020000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.582"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..538)
                     /locus_tag="ACHOQQ_RS25685"
                     /old_locus_tag="pgaptmp_005137"
     CDS             complement(<1..538)
                     /locus_tag="ACHOQQ_RS25685"
                     /old_locus_tag="pgaptmp_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4473242.1"
                     /translation="MKGLSSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKAN"
     gene            complement(618..>1142)
                     /gene="yehD"
                     /locus_tag="ACHOQQ_RS25690"
                     /old_locus_tag="pgaptmp_005138"
     CDS             complement(618..>1142)
                     /gene="yehD"
                     /locus_tag="ACHOQQ_RS25690"
                     /old_locus_tag="pgaptmp_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4473243.1"
                     /translation="AAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSV
                     SINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDK
                     AIASSNDTVGYYLYLGDNSEVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDSSVNS
                     GDVLSSLVMRVAQD"
BASE COUNT          324 a          252 c          208 g          358 t
ORIGIN      
        1 tattagcttt gacatccgaa atcgtcaccc aattagctga gtcatttttt atcaccaatc
       61 cattgccact gcgatttacc agtaactttt taaaggtcgc tttatttacc ggagcaatac
      121 ccgccggccg gtaaaacaat ttgattctgt tttgcatcgc aaacttcagt gcattcttac
      181 cttcttgttc tggactattt ggtggaatat ccagaacatt cagataaaaa atgctttctt
      241 tattagtggg cagtttattc ggcataattt tgatttttac ttgctgcccg gaatttgccc
      301 ctatttttgc cactggtggc gttaacatga aaggaacctg aattttttct ggtggtaatg
      361 acgtatcgcc atcatcaatc cacgcctgca gcagcgaaga acggtttccc tggttcatca
      421 actgcaccat cacttcttta ttttctgccg ggtaaataat gcgcgtcccg tagataacaa
      481 ttccggcatg tgcaggaagg accatagaaa aaatgagtaa agatgataat cctttcattt
      541 taatgcctct taaattaaaa ggccgccatg ggatagcggc catgtttatt acctggagaa
      601 acttacaatt ttatttatta atcctgcgcc acacgcataa ccagagaaga taacacatca
      661 cctgaattca ctgagctatc tgtcagtttc aggtattttg ctttaaacgg aatagcatat
      721 tgaccttccg ctgttttata actctcaata ttaaaaggtg cagaaacatc caggacttcg
      781 ctgttatcac ctaaatagag atagtaaccc acagtatcgt tgctactggc tatcgcctta
      841 tcgttaccag ttattttatc gttggtaatc aacgagagtt ttacaccagc aacttctttc
      901 gggcagctaa tttttaacat gctgctggtt acaccgactg catcatcata ggtagaatat
      961 ttagctttac cttcaaagac agtggttggt acagttggca tattaatact tactgaatca
     1021 ccacccgctt cgaatttaca cggagattct gcaatatttc ctttaatggt taatgttccg
     1081 gtatcaacgt ctgcagcaaa tactccagcg ctcataaaaa aagaagaaaa gacagcggca
     1141 gc
//
LOCUS       DAZKFU020000573         1140 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZKFU020000573 DAZKFU020000000
VERSION     DAZKFU020000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.583"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..359)
                     /gene="gpFI"
                     /locus_tag="ACHOQQ_RS25695"
                     /old_locus_tag="pgaptmp_005139"
     CDS             complement(<1..359)
                     /gene="gpFI"
                     /locus_tag="ACHOQQ_RS25695"
                     /old_locus_tag="pgaptmp_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4473244.1"
                     /translation="MATKEENQKRLRELAGLLGREADMSGSAADIAQRVAEWEEEVCA
                     SENEIADVDDTVYEQAYRNTGEDALGILERIRLLKCFYLCGVDDETGEPVEHVDAGRV
                     ILMPPSVAKDMVRSGMAV"
     gene            complement(412..>1140)
                     /locus_tag="ACHOQQ_RS25700"
                     /old_locus_tag="pgaptmp_005140"
     CDS             complement(412..>1140)
                     /locus_tag="ACHOQQ_RS25700"
                     /old_locus_tag="pgaptmp_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4473245.1"
                     /translation="LNDPTYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEG
                     DQFEKIEVDFGRSTKNNIIQGSGKEWSKQDRDTFDPTYDIDLFCDQASGLVNIAIMDG
                     TVWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGSVVSFKGYYGDLAIVVAKTSYVAE
                     DGTEKRYLPEGTLVLGNTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPARE
                     FTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          256 a          360 c          249 g          275 t
ORIGIN      
        1 acggccattc cactcctgac catgtctttt gccactgagg ggggcatcag aattactctg
       61 ccagcatcaa catgctcaac aggctcacct gtttcatcgt caacgccgca caggtaaaaa
      121 cacttcagaa gcctgatacg ttccagaata cctaaggcat cctcaccggt gttcctgtat
      181 gcctgctcat aaacggtatc atcaacatct gcgatttcat tttccgatgc gcaaacctcc
      241 tcttcccact ctgccacacg ctgtgcgata tccgctgcac tccccgacat atccgcctcg
      301 cgccccagca ggccagccag ttcacgaaga cgtttctgat tttcttcttt tgttgccata
      361 tccagccccc tgtgaaaaaa gacacggggg catttcgccc ccgctcacgg attatttcac
      421 ctgtaccacc acaaactcat ccggatccgg cagcaccatc agcggagcgg actgcgtcat
      481 ggtgaattca cgggccggat cgcccacggt catccagtgt ttcggataac gggaagaggc
      541 caccacacct tcggacaacg cctgcgcatc ctgaatggca ccatagcaac ggatgccctc
      601 agcagccgta tttcccagga ccagcgtgcc ctccggcaga taacgttttt cggtaccgtc
      661 ctctgccaca taagacgttt tcgccaccac aatggccaga tcgccgtaat accccttgaa
      721 agacaccacc gaccccaggt ctttcactgc cgtttcgagt tgtgaatttg agccgcgacg
      781 ggtatccagt ttttcgcgga acagcttaaa gccattcagc agacgccaga cggtaccgtc
      841 catgatggca atattcacaa gaccgctggc ctgatcacag aaaaggtcga tatcatatgt
      901 cgggtcgaac gtgtcacggt cctgttttga ccactcctta ccgctaccct gtatgatgtt
      961 attcttcgtc gatctgccaa aatcaacctc aattttctcg aactggtctc cttccatggt
     1021 gtatttgcca tacagcacgg cattcaccgc ctgcatttcc tccacctgga caatggcgtg
     1081 ctcttcctgt ttgaggttat cggtgatgat acgcagacga cggtaggtcg ggtcgttcag
//
LOCUS       DAZKFU020000574         1136 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZKFU020000574 DAZKFU020000000
VERSION     DAZKFU020000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.584"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..229
                     /gene="yniD"
                     /locus_tag="ACHOQQ_RS25705"
                     /old_locus_tag="pgaptmp_005141"
     CDS             122..229
                     /gene="yniD"
                     /locus_tag="ACHOQQ_RS25705"
                     /old_locus_tag="pgaptmp_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGU4473246.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(282..1040)
                     /locus_tag="ACHOQQ_RS25710"
                     /old_locus_tag="pgaptmp_005142"
     CDS             complement(282..1040)
                     /locus_tag="ACHOQQ_RS25710"
                     /old_locus_tag="pgaptmp_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGU4473247.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          315 a          281 c          268 g          272 t
ORIGIN      
        1 cgcagaaaca gtgatcaggt gtacaacgcc acccgacggc tgtaataaca aaaaatccct
       61 tgatgcctgt cccttttgtt acactccgtt atcacgcaca agagatatgc aggacactgg
      121 tatgccgact aaacgctttg ataaaaaaca ctggaagatg gtggtggtgc tactggcaat
      181 ctgtggcgct atgttgttgc tacgttgggc agcaatgatt tggggctgag cagtgacaaa
      241 cgaaagcagc cagaccgcat aacacgatcc ggcagataat tttacatgct gtagcccagc
      301 gataaggttg tccgacgatc ggtatgttcc ggtgcggact ctggaggttc agagttccat
      361 gtgacgttgt aagccacctt cagtccaaaa tgttcattaa tagcaacatt taatgcactt
      421 tcggagttca gcgttgtgtc ttccgcgcca aagaccgaaa caccctgcgt aaatttagcg
      481 ttgtcagtca actgccatgc ataagccccg gaggcatagc ccagcggctg agtttcactg
      541 gcattgtcgg tatatttgtc gtaacgcaca cctggaccga attcaaagcg gaaactgtgt
      601 accgggccat tcaaaaactg acgaccataa cccgcggtca acacatcgcg ctctcgatag
      661 ccgttataac ggtctgtcag ccagcttgcc tgaccaaata aatagtcata atcagttaaa
      721 ttaaaacggc tacgcccgcc cgccgcatat ttttctgaag aacgctcatc attagaagaa
      781 gtattactgg cgttccccca cagcgaccag gcagtggttt gtccatacca ggtcatggtg
      841 gtgtcagccg taagtgagga gcttttcgta ttgcctgatt gtgcaagata tcctgcgttc
      901 agattacctt cgaaaggttt tttagcgctg gcaggatcgt ccatgacagt aaaaacggaa
      961 tcatcggcag ctgcattcag tgacgcaaac atgccccccg ccaacataac gatggcagga
     1021 actgtcttca aaagcttcat ttatcaagag tccgtacaac aaaaaaagag accatcgcgg
     1081 tcccggaaac tttcttaagg atcaaagatt agcgtccctg gaaaggtaac gaatta
//
LOCUS       DAZKFU020000575         1122 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZKFU020000575 DAZKFU020000000
VERSION     DAZKFU020000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.585"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..641)
                     /locus_tag="ACHOQQ_RS25715"
                     /old_locus_tag="pgaptmp_005143"
     CDS             complement(<1..641)
                     /locus_tag="ACHOQQ_RS25715"
                     /old_locus_tag="pgaptmp_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HGU4473248.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKT"
     gene            838..>1122
                     /locus_tag="ACHOQQ_RS25720"
                     /old_locus_tag="pgaptmp_005144"
     CDS             838..>1122
                     /locus_tag="ACHOQQ_RS25720"
                     /old_locus_tag="pgaptmp_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473249.1"
                     /translation="MNTEKQLPSGIESEVKYTLQFWLLSNGKVMTEKDGLCNPFPLNC
                     IESAHPFTPFQQNQWVELDTVGPNGPVKATFLVHSVLYKLKRQGGSLNDAG"
BASE COUNT          275 a          316 c          292 g          239 t
ORIGIN      
        1 gttttcccca ttgacggacg ggcagccagc agaaccaggt caccaggctg caggccacag
       61 gtcatttcat ccagacagtc aaatcccgtg ggggtaccgg taacaccacc ggagccgagc
      121 acactttcca gttgctgcag aacggtatca agcgcggatg aaagagaggc ttccgtgcgt
      181 tcccggagcg tcctgttctg cgcaagtgca aacagacgcc gctctgcagc atccagcaca
      241 tcccgtgatg tggcgttcgg tgccgccaca tcagcaatca gggtgtttcc ggtggcctgt
      301 aactgacgca gaacgctttt ctccaccaca atgcctgcat aggccatgat gttggccgca
      361 gaaggtgtgt tcttgctgag ctcggccaga taagcaaagc cgcccagtac gtccagcctg
      421 ccctgatgtt ccaggtgctc tgacagtgta atcaggtcca gtggctggcc attcaccgcc
      481 agttcagaca tggcacggaa aatagcccgg tgctgagcca cataaaaatc gtcaggactc
      541 agcatcagca ccacttcatc ccagcgttca ttctccagca tcagaccgcc cagaacggcc
      601 tgctctgctt cgtatgcgca gagaacagac atttcagcca tggcgccccc tccccctctg
      661 acagccggaa acctggctac gtgaaaaaag gaaaaagggt gccggacgta cagctgtcgg
      721 gaataaaatc ccggtaactg tctgtacgtt ttcagaccgg tgagtttgcc gcccccggtc
      781 tgaaggtacg gacagcattc gttcattcat aacaagttgt tttaaaggaa aaagtttatg
      841 aatacagaaa aacaactgcc gtccggcatt gaatctgaag tgaaatacac cctgcagttc
      901 tggctgctga gtaacggaaa ggtcatgacg gaaaaagacg ggctctgtaa cccctttccc
      961 ctgaactgta ttgaatcggc tcatcctttt actccgtttc agcagaatca gtgggttgaa
     1021 ctggacactg tcgggccgaa tggaccggtg aaagcgacat tccttgtgca cagcgttctg
     1081 tataaactga aacgtcaggg tggctccctg aatgatgccg gc
//
LOCUS       DAZKFU020000576         1118 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZKFU020000576 DAZKFU020000000
VERSION     DAZKFU020000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.586"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..610)
                     /locus_tag="ACHOQQ_RS25725"
                     /old_locus_tag="pgaptmp_005145"
     CDS             complement(<1..610)
                     /locus_tag="ACHOQQ_RS25725"
                     /old_locus_tag="pgaptmp_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473250.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDALVNNPVKTLAGLSSFMTDVYMPPS
                     STQKELGAPPSVGDAIKNIPDGTGKNAVTDTVGKGLKVTGKSVSDGAKASDEWLTGKM
                     SPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVVGGVA"
     gene            complement(696..>1118)
                     /locus_tag="ACHOQQ_RS25730"
                     /old_locus_tag="pgaptmp_005146"
     CDS             complement(696..>1118)
                     /locus_tag="ACHOQQ_RS25730"
                     /old_locus_tag="pgaptmp_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473251.1"
                     /translation="AEISKNYGYFMPKQQGLSLKELQKEASKVVAQAIKDGRDAQAAN
                     DEFLAAYDSLPHQQKVQQRKTQQLLQKWQPWAAGDPVKQEFVKEAAANAPALLEPGRE
                     KEVEIYYQNHLRDKKAEHEKALDYSASAAAEKIKGWMK"
BASE COUNT          256 a          326 c          245 g          291 t
ORIGIN      
        1 tagccacacc gccaacaacc atatcaggta caagcgcacc aatcaggttt acacccttcg
       61 ccacccagac cgcagaatca tcatagcctt cggtcatcgg cgtattcagc gcacgaacag
      121 cacccggcga catcttaccg gtcagccatt catccgaggc tttagcacca tcactgactg
      181 acttgcctgt gaccttcaga cctttaccga cagtatcagt caccgcgttt ttccctgttc
      241 catcgggaat gtttttaatc gcatctccaa cagaaggtgg tgcgcccagt tccttttgcg
      301 tggaggaagg cggcatgtaa acatccgtca tgaaagacga caacccggca agcgttttta
      361 caggattatt tacaagtgca tcaccgagtc cacgggccat ttcccctgtt ccccggacgg
      421 actgggcgaa acctttaccc attgttggca atacatcacc gaggctgaac gatgtgctgt
      481 gatcaatggc attctgcggg ttgctgaaaa aatcctgcca gtaattttct cccggctgct
      541 taatgttcag gctgctgcgg ttctggttac ccaattgcgc ttcagggcgc tgctcatctg
      601 aaaaagccat acagactcca gtaaaaaaaa gcccgacacg atggtcgggc gtaagtgaac
      661 gatattaacg gggagcgcgt tactgccgca tatctttact tcatccagcc tttgatcttc
      721 tcggcagctg cggaagcgct ataatccagc gccttttcat gctctgcctt tttatcccgg
      781 aggtgattct ggtaataaat ttccacctcc ttttcacgac caggttccag caaagcaggc
      841 gcattggcgg cagcctcttt tacaaactcc tgtttaaccg gatcgcccgc cgcccacggc
      901 tgccacttct gtaaaagctg ttgcgttttc cgttgctgaa ctttctgttg atgcggcagt
      961 gaatcatacg ctgctaaaaa ctcatcattc gcagcctgag catcacggcc atccttgatc
     1021 gcctgagcca ctactttgct ggcttccttc tgaagctctt tcagagaaag tccctgctgc
     1081 ttcggcatga aataaccata atttttcgat atctctgc
//
LOCUS       DAZKFU020000577         1109 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZKFU020000577 DAZKFU020000000
VERSION     DAZKFU020000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.587"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1109
                     /locus_tag="ACHOQQ_RS25735"
                     /old_locus_tag="pgaptmp_005147"
     CDS             <1..>1109
                     /locus_tag="ACHOQQ_RS25735"
                     /old_locus_tag="pgaptmp_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HGU4473252.1"
                     /translation="ILSIDAASRTLTLDREVILPEAGTSTVNLINGSGKPVRVDITAH
                     PAPDRIQVSVLPDGMETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKE
                     AIVDNGARFEPMSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLR
                     LTSGSGENSRLVTSAITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAP
                     AAPASIELTPGYYQITAVPHLTVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGRQW
                     SVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQG
                     MWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTL"
BASE COUNT          245 a          309 c          351 g          204 t
ORIGIN      
        1 atcctgtcca tcgatgccgc cagccgtacc ctgacgctgg accgtgaggt gatactgccg
       61 gaagcaggga catcgacggt gaacctgatt aacggcagcg gtaagccggt gcgcgtggac
      121 atcactgcac accccgcccc tgaccggata caggtcagcg tcctgccgga tggcatggag
      181 acatacggtg tgtggggact ctccctgccg tcactgcgtc gtcgcctgtt ccgctgtgtt
      241 tccatccggg aaaacacgga cggtaccttt gccatcacgg cggtgcagca tgtgccggaa
      301 aaagaagcca ttgtggataa cggggcccgc tttgagccga tgtccggctc actgaacagc
      361 gtcatcccgc cggcggtgca gcaccttacg gtggaggtga gtgcctcaga cggccagtat
      421 ctggcactgg cgaaatggga cacgccgcgg gtggtgaagg gcgtgcgctt cagtctgcgc
      481 ctgaccagtg gcagtggtga aaacagccgc ctggtgacca gcgccatcac ggcggacacg
      541 gagcaccgtt tcagtggcct gccgcccggg gaatacaccc tgacggtcag ggcgataaac
      601 agctacggcc agcagggcga acctgccacc accacgttcc ggattaacgc accggcagca
      661 ccggccagca ttgaactgac gccggggtat tatcagataa cggcggtccc gcatcttacg
      721 gtgtatgacc cgacggtaca atttgagttc tggttttcgg aggcaaaaat cgcagacaca
      781 tctcaggtgg aaacctctgc ccgttatctg gggacaggcc gccagtggag tgtctccggc
      841 ccgcacatta agccggggaa ggatttctgg ttttatgtgc gcagcgtcaa cctggtgggg
      901 aaatctgcgt ttgtggaagc cagtggccgg gcgagcaatg atgcggaagg gtatctggac
      961 tttttcagag gagaaatcgg gaagacacat ctggcacagg ggatgtggga gctgattgat
     1021 aacagccagc ttgatgatga gatggcggag atgaagacca ccatcaccga aacccgcaat
     1081 gaaatcacgc agacggtcag taaaaccct
//
LOCUS       DAZKFU020000578         1107 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZKFU020000578 DAZKFU020000000
VERSION     DAZKFU020000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.588"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(177..464)
                     /locus_tag="ACHOQQ_RS25740"
                     /old_locus_tag="pgaptmp_005148"
     CDS             complement(177..464)
                     /locus_tag="ACHOQQ_RS25740"
                     /old_locus_tag="pgaptmp_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGU4473253.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
     gene            complement(539..682)
                     /gene="mgrB"
                     /locus_tag="ACHOQQ_RS25745"
                     /old_locus_tag="pgaptmp_005149"
     CDS             complement(539..682)
                     /gene="mgrB"
                     /locus_tag="ACHOQQ_RS25745"
                     /old_locus_tag="pgaptmp_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGU4473254.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            841..1080
                     /locus_tag="ACHOQQ_RS25750"
                     /old_locus_tag="pgaptmp_005150"
     CDS             841..1080
                     /locus_tag="ACHOQQ_RS25750"
                     /old_locus_tag="pgaptmp_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGU4473255.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
BASE COUNT          341 a          241 c          223 g          302 t
ORIGIN      
        1 taaattaatg aggaaaatat ctttttctga tatcccgtta cgcaaataaa taatatgata
       61 attatttgaa gtcagaccag agttttaatt tgaagcagtt gcaccatgac agtgcgtata
      121 atcaggatga attgagtaac ccttcaacga cggggttact caagattgag gaaggattat
      181 cgttcggcga ccaggcgaat aatatcttcg tcttcaaccg atttttgact ctcaatggtc
      241 tttttatctg ctttctctgg ttcgtttgct tcgcacaaac gacgtaacag tgcattttga
      301 cgtttttgct gatccagcaa cgcctcaagc agttcaatct gttcgttagt ccgagaactg
      361 gcacgattaa taaaaaacca caagatgagc ccgataagaa gaaccaccac cgatacaacc
      421 aaagacgcaa tgttcatcac gcctgaattt acaacttcgt tcatttcacc acctctgagt
      481 agaggcgcta ttctaccact gctggagagg aagaaaatct agtgctgaaa aaatgatatc
      541 accacgggat aaactggtta atggcacaaa ttccgctgaa aaattgtaca tcctgatcgc
      601 acatcatgtt gaatacctgc gcccaaagca gcaagcaagc caacaccacg acaaccagaa
      661 cgacccatcg aaactttttc actccaatct ccgtttcacc taccttatgt cattaaacta
      721 gcatgcgtta gttaaacagc gcctaactat gtcgatattc gtgctttttc ggaatgagtc
      781 gcttgcttca gcgcatattg ccgctacgat taagcgaaca taaaaagaga agaggttgta
      841 atgcgattca tcattcgcac agttatgctg atagcactgg tatggatagg attattactc
      901 agcggatatg gcgttttgat tggaagcaaa gagaatgccg caggattagg tttgcaatgt
      961 acctatctga ctgccagagg caccagcacg gtacaatact tgcacactaa aagtggtttt
     1021 ttggggataa cagattgccc gctgttacgc aaaagcaata tcgtggtcga taatggttaa
     1081 gtgtaataaa taaccagatt ctcactc
//
LOCUS       DAZKFU020000579         1100 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZKFU020000579 DAZKFU020000000
VERSION     DAZKFU020000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.589"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..683)
                     /gene="tssI"
                     /locus_tag="ACHOQQ_RS25755"
                     /old_locus_tag="pgaptmp_005151"
     CDS             complement(<1..683)
                     /gene="tssI"
                     /locus_tag="ACHOQQ_RS25755"
                     /old_locus_tag="pgaptmp_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HGU4473256.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTCTEVSTLCISQFRYSA
                     QIRPSSVVT"
BASE COUNT          308 a          267 c          266 g          259 t
ORIGIN      
        1 ttggtcacca cggaagaagg gcggatttgt gcgctgtagc ggaactggct gatgcagagg
       61 gtgcttacct cggtacaggt gttcggattc caggggatct caaaggactc cggcagataa
      121 cgcacggtgt cgcacaggac caggctctgg tcggtacttt tttgcgcgtg ctcctcataa
      181 aagaagatgc cttcctccgc cgccatccgg cacaggaaat cgtaatcagt ctcaccgtac
      241 tggacacaaa actcacggga aggatgcggt tcactgaaca gcggactcca ctcggtcacc
      301 ccgttttcct gcaatatcgt gccgaggatg ctttcgatgt cctcattctg gaagatacgg
      361 aagttctggc gcagccctgt gcgccacagc ggtgggcaca ccttcatgct gtacagcatc
      421 tggtttttgt cgttctcccc cagttcaaac caggtcacca cacctttcac ccggcgctgt
      481 acgtcatcgc cctgccatac cgtcaggtag gccattttgt ccagcacctg cgcaaattca
      541 agggaaagaa actgctgact gaccagagaa aggtcgaggg aaaaaagtga agagagtgac
      601 tgggtgagat gaaaggaaac caccgcaaaa gcatccggtg gcaggccgtc cacttccagc
      661 gtgaaacgta atccggttga catagcaacc tccgtgaaaa tgaataataa tccgcacata
      721 ttgcatgtac aacatgtcgg gcggatgaga taagcgagtc gacatctggc gttaccgggg
      781 aatatcagaa aaatgtcatg gttatgtgat gatttagtct gggattttac ttcagagaaa
      841 cgggtgtctg tttagtcaac aaaataatca caaacgcaac ggatgggttc tcgacgttat
      901 tcacgacagg ctcgtacatt acagcgagta ataaaaaacc tgatatgcaa agtcaaacca
      961 atattcataa tgtcttttta ttcacaacaa taaataattt aataacaatc attgaaatga
     1021 atgatgattt gtataaggaa tatacgctac cgccccaccc tgataacatg atctgcacat
     1081 tgccatctta acgtcgttaa
//
LOCUS       DAZKFU020000580         1099 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZKFU020000580 DAZKFU020000000
VERSION     DAZKFU020000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.590"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1099
                     /locus_tag="ACHOQQ_RS25760"
                     /old_locus_tag="pgaptmp_005152"
     CDS             <1..>1099
                     /locus_tag="ACHOQQ_RS25760"
                     /old_locus_tag="pgaptmp_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HGU4473257.1"
                     /translation="FSCGTCQATGAEVTSESVFNILNSTGAATDKSYLSLNPDKYPNY
                     RLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGTFILASTFEDDKTAA
                     ETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPDQIKRMQDIISNPKR
                     LDGIPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGLYLDLLIQAINTGTI
                     NAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTSSVALVTSGLERLSN
                     LLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITLQLDLGGESPGYLEK
                     DKHYREADAALLNVIYPANLSKINTRRKEQVL"
BASE COUNT          356 a          219 c          245 g          279 t
ORIGIN      
        1 tattttcgtg cggcacatgc caggcgacag gggcagaagt gacgagtgaa tctgtattta
       61 acattcttaa ttccacgggg gcagcaacag ataaaagtta tttatcgtta aatccagata
      121 agtatcctaa ttatcgtctt ctgattcact cagccaaatt acaaaatgaa ataaagagcc
      181 actataccaa agacgaaatt caaggattgc taacattaac agaaaacaca cgaaagttaa
      241 cgttgacgga gaaaccctgg ggaacgttta ttcttgcttc tactttcgaa gatgacaaaa
      301 cagcggcaga aacccactat gatgctgtct ggctgaggga tagtttatgg ggctatatgg
      361 cattagtatc agaccaggga aatagtgtag ccgcaaaaaa ggttctgcta accctgtggg
      421 attatatgtc tacgcccgac cagattaagc ggatgcagga catcattagc aacccaaaac
      481 gactggatgg tattcccggg caaatgaatg ctgtgcatat ccgatttgac agcaactccc
      541 ccgtgatggc agatgtgcag gaagaaggca agccgcagct gtggaaccat aaacaaaatg
      601 atgcgttagg tctttatctt gatctcctta tacaagcaat caatactggt actattaacg
      661 ctgaagattg gcaaaaaggt gaccgactaa aatcggttgc gctgctgatt gcttatctgg
      721 ataaagccaa tttctatgtt atggaagatt ccggagcgtg ggaggaagat gcccgattaa
      781 acacatcttc cgttgcactg gtgacctctg gactggaacg tttgtcaaac ctactctcga
      841 agaaagactc tgtttttgtc tctgatttac tacgcgaagc taaagtcaat gaactggatg
      901 aaactctttc aacaacgagg ttgaaccacc tcatagataa aggttatgaa agaataacgt
      961 tacagcttga tttaggggga gaatccccag gttatctgga aaaagataaa cattacagag
     1021 aagccgatgc ggctttactt aatgttattt atcctgcaaa tctttcaaag ataaacacca
     1081 gaagaaaaga gcaagttct
//
LOCUS       DAZKFU020000581         1098 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZKFU020000581 DAZKFU020000000
VERSION     DAZKFU020000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.591"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(57..>1098)
                     /locus_tag="ACHOQQ_RS25765"
                     /old_locus_tag="pgaptmp_005153"
     CDS             complement(57..>1098)
                     /locus_tag="ACHOQQ_RS25765"
                     /old_locus_tag="pgaptmp_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4473258.1"
                     /translation="LGLPCGYAANDGTCATRGGTHTLSLNFPLTTVSAANNVPGNTLI
                     DIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTTASGLAFYYLNEYVD
                     VGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLKTGADAKISFRIKRS
                     INGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSCQINAGQVIYFDFDT
                     IPASEFSSTAGQAITSRKITKTVSIECTGMGDERTQKVDASFTGTNRSSDDTMVATDN
                     ADVGIKIYNKSNAEVSVNNGKLPADMGNTTIIGRKNGSVTFSAAPASFTGARPQPGVF
                     NATATLTIEFVN"
BASE COUNT          292 a          248 c          241 g          317 t
ORIGIN      
        1 ttttttcatg aaaggagagg aaatatgaac ggctgtcata aaacagccgc aaaaggttag
       61 tttacaaatt caatggttaa ggtcgcggta gcgttaaaaa cgccgggctg aggccgggca
      121 ccggtaaagc tggcaggtgc tgccgaaaaa gttaccgaac catttttacg accaataatg
      181 gtcgtgttgc ccatgtctgc gggtaacttg ccgttgttga cgctaacttc agcattcgat
      241 ttattgtaaa ttttgatccc gacatcagca ttgtctgtcg ccaccatcgt atcgtcactg
      301 cttcggttcg tccccgtaaa agaagcatcg actttctgcg tacgctcatc ccccatcccc
      361 gtacactcaa tactcactgt tttagtgatt tttcgtgaag taatggcttg cccggcggta
      421 gatgaaaatt cggacgcagg aatagtatca aaatcaaaat aaatcacctg tcctgcattt
      481 atctgacaag actgtggtgc ggtcaaactg cctgaaattc gaacttttgc aatcgcctca
      541 ccacgggtcg tggtgctgga tatgttggca tacagcaatg caatatcggt gataggtatt
      601 actaccgttc catttattga acgtttaata cgaaatgaaa tcttcgcatc tgctccagtt
      661 ttcaggctca cgccaaccgc tgtagttttg tttccctgac aagtgtgatc tgttgtagtt
      721 gcctggttgg aaacatgttc aaaaggaact gccgtatacc ccgcatttag cacagatatt
      781 tttgttccca catcgacata ttcgttaaga tagtaaaaag caagaccact tgcggtggtg
      841 ctataaacca taccgggagc agggtctgcg gtataatata tttcgtgata agcgccattg
      901 ctatgttttg aatcacagtt acacagaacg ctataatttt cagaagatgt tgcattagca
      961 atatctatta atgtatttcc aggcacattg tttgctgcac tgaccgttgt cagaggaaaa
     1021 ttaaggctta atgtatgtgt gccgcctctt gttgcacacg tgccatcatt tgccgcatac
     1081 ccgcacggca accctaaa
//
LOCUS       DAZKFU020000582         1097 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZKFU020000582 DAZKFU020000000
VERSION     DAZKFU020000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.592"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            77..1051
                     /gene="cysB"
                     /locus_tag="ACHOQQ_RS25770"
                     /old_locus_tag="pgaptmp_005154"
     CDS             77..1051
                     /gene="cysB"
                     /locus_tag="ACHOQQ_RS25770"
                     /old_locus_tag="pgaptmp_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGU4473259.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          285 a          261 c          266 g          285 t
ORIGIN      
        1 tattaaatcg tattagtcac ccgccaggtg tgacgaaaaa acgatgttct gatggcgtct
       61 aagtggatgg tttaacatga aattacaaca acttcgctat attgttgagg tggtcaatca
      121 taacctgaat gtctcatcaa cagcggaagg actttacaca tcacaacccg ggatcagtaa
      181 acaagtcaga atgctggaag acgagctagg cattcaaatt ttttcccgaa gcggcaagca
      241 cctgacgcag gtaacgccag caggacaaga aataattcgt atcgctcgcg aagtcctgtc
      301 gaaagttgat gccataaaat cggttgccgg tgagcacacc tggccggata aaggctcgct
      361 gtatatcgcc accacgcata cccaggcacg ctacgcgtta ccaaacgtca tcaaaggctt
      421 tattgagcgt tatccccgcg tttctttgca tatgcaccag ggctcgccga cacaaattgc
      481 tgatgccgtc tctaaaggta atgcagattt cgctatcgcc acagaagcgc tgcatctgta
      541 tgaagattta gtgatgttac cgtgctacca ctggaatcgg gctattgtag tcactccgga
      601 tcacccgctg gcaggcaaaa aagccattac cattgaagaa ctggcgcaat atccgttggt
      661 gacatatacc ttcggcttta ccggacgttc agaactggat actgcgttta accgcgcagg
      721 gttaacgccg cgtatcgtct tcacggcaac ggatgctgac gtcattaaaa cttacgtccg
      781 gttagggctg ggggtagggg tcattgccag catggcggtg gatccggtcg ccgatcccga
      841 ccttgtgcgc gttgatgctc acgatatctt cagccacagt acaaccaaaa ttggttttcg
      901 ccgtagtact tttttgcgca gttatatgta tgatttcatt cagcgttttg caccgcattt
      961 aacgcgtgat gtcgttgatg cggctgtcgc attgcgctcc aatgaagaaa ttgaggtcat
     1021 gtttaaagat ataaaactgc cggaaaaata atttcttgcg ttattttcgg cacctttttt
     1081 gtagcgaagg tgccgaa
//
LOCUS       DAZKFU020000583         1096 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZKFU020000583 DAZKFU020000000
VERSION     DAZKFU020000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.593"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..134)
                     /locus_tag="ACHOQQ_RS25775"
                     /old_locus_tag="pgaptmp_005155"
     CDS             complement(<1..134)
                     /locus_tag="ACHOQQ_RS25775"
                     /old_locus_tag="pgaptmp_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4473260.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     V"
     gene            73..255
                     /locus_tag="ACHOQQ_RS25780"
                     /old_locus_tag="pgaptmp_005156"
     CDS             73..255
                     /locus_tag="ACHOQQ_RS25780"
                     /old_locus_tag="pgaptmp_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473261.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     IYLLQKMNITDKTKYK"
     gene            675..868
                     /locus_tag="ACHOQQ_RS25785"
                     /old_locus_tag="pgaptmp_005157"
                     /pseudo
     CDS             675..868
                     /locus_tag="ACHOQQ_RS25785"
                     /old_locus_tag="pgaptmp_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          328 a          235 c          250 g          283 t
ORIGIN      
        1 acactggcgg ctgcgttggt cagttttttc tcatagccgg atgccgagac aatcatcaca
       61 tcctctgctg ccatgctgct ggctgaaaac ccgagacagg gaatgacaac ggaagccaga
      121 gtggttattc gcatattact aatctccgat catgttaatc ggcaagacaa tacacaaata
      181 caaacaataa tgagaataat ttatatttac cttttacaaa aaatgaatat tactgacaaa
      241 actaaatata aataatgact gcctcggcat gccagttaac agccatccgc aatggcgtgt
      301 tcggttttgc gaataaaaaa tggttactgc agaagcggat tttcaccacc tgtcaaccat
      361 gaatggtcag tggagtgaga cgactggtat gatttggtcg atgacttctt ccggtgaata
      421 cggacagaaa cattgaaata ttgtaagaga gaacgttcag aattctgtgt cacgtgaaaa
      481 tactacagcg ttatcacctt attcagtcga ccttcaaaat aaacgtctaa ccgtgacaat
      541 caccatttgg attgcccact ggggaggcga acgattgcct cgacggcatt tataatgcca
      601 gcccacattg ttggcgctga caatactaag gacaaacgat gagggagaac cgtcttatac
      661 cactagcgct gacaccggca caccttcccc gtgaaataca ccggctggag cctgaagaaa
      721 gctgttgccc ggagtgtggc ggtgagctgg attatctggg gaagtcagcg ctgaacaact
      781 ggaactggtg agcagctccc tgaaagtgat cagcacagaa cgggtaaaaa aagcctgtaa
      841 aaaatgtgac ggtaccgttg aagcgccggt ctcgtaagaa taatcctgga caaataagat
      901 ttttcctgaa aaagcgtcta gtcttttgat atcgttgttt ttatgctaat cagggagtat
      961 gtgtgatgat tgtctgttac gttattcttg ttaaccgtcc gtttgaaaca gagattgccg
     1021 ggaaaaagct attttttcag aaaaatgacg tcctgttgac agataacatc agccagtcct
     1081 ttttcctgcg ccatcc
//
LOCUS       DAZKFU020000584         1094 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZKFU020000584 DAZKFU020000000
VERSION     DAZKFU020000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.594"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..252
                     /locus_tag="ACHOQQ_RS25790"
                     /old_locus_tag="pgaptmp_005158"
     CDS             <1..252
                     /locus_tag="ACHOQQ_RS25790"
                     /old_locus_tag="pgaptmp_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HGU4473262.1"
                     /translation="TNEELTSFSSHEKFSVVPGFDLTTIADSVIRKKVSLINKSRILE
                     DYSVTELRISASEIGVALEAEKIGEIEKIKMPQIRKDVN"
     gene            781..>1094
                     /gene="subA"
                     /locus_tag="ACHOQQ_RS25795"
                     /old_locus_tag="pgaptmp_005159"
     CDS             781..>1094
                     /gene="subA"
                     /locus_tag="ACHOQQ_RS25795"
                     /old_locus_tag="pgaptmp_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HGU4473263.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHR
                     VYG"
BASE COUNT          322 a          203 c          225 g          344 t
ORIGIN      
        1 actaatgaag aattaacaag tttttcttct catgaaaaat tttcagtagt tccagggttt
       61 gatttaacaa caattgctga ctctgttatt agaaagaaag tttccttaat taataaatca
      121 agaatacttg aagactactc tgtgactgag ttaagaattt cagcgtctga aattggtgtt
      181 gctctagaag ctgaaaaaat aggtgaaatt gaaaaaataa aaatgccaca aataagaaaa
      241 gatgtaaatt aaggaaacaa catgttgggt ggagcggaca atccaaatgg cgaattaccg
      301 tcttatatca ctggcgctga cacctgatat ccggggctta ctgcacacat aataatgagc
      361 cagggtaaac gacacaacca tgcgctgatc gctccggtaa gacggcactg taatttcctg
      421 ttcgccatga tgcatgacgg tactctttat tgtcataaac atgccaggca acatagttgc
      481 cccctcttat tttttctggc taatttttac ccatcatagc tggatttact tctgctctga
      541 aggagtagga agagcatctc accgattacc tattgcagaa atcctggtgc atattaccgt
      601 gaaggagttc agtgagctat tgaatatgca aataaataaa ttaatatttt tgagaatgac
      661 ttcactcgct ttattgattg tgattgttta tcttttgtcg aaaacaggat atttcagatg
      721 tgatgttcct gtaagatatt gacctgcaat actgtttatt tgtactgact ggaggatatt
      781 gtgcttaaga ttttatggcc gcatattctg ttcctgcttt ttatatctgt gtctgtcagg
      841 gcagaaaaac cctggtattt tgatgcaatt ggcctgacag aaacaaccat gtcgctcaca
      901 gacaaaaata ccccggttgt cgtatctgtt gttgattccg gagtggcatt tgtaggaggc
      961 ctcagcgaca gcgaatttgc caaattcagt tttactcagg atggttcgcc attcccggta
     1021 aaagagtctg aagctttata tattcatggt actgccatgg cttcactcat tgcctcacgt
     1081 catagggttt atgg
//
LOCUS       DAZKFU020000585         1094 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZKFU020000585 DAZKFU020000000
VERSION     DAZKFU020000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.595"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            186..>1094
                     /locus_tag="ACHOQQ_RS25800"
                     /old_locus_tag="pgaptmp_005160"
     CDS             186..>1094
                     /locus_tag="ACHOQQ_RS25800"
                     /old_locus_tag="pgaptmp_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473264.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDPLKTDEESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEEGHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETNLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDILLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALV"
BASE COUNT          342 a          220 c          202 g          330 t
ORIGIN      
        1 tgtttcatta gtaataatca cttaaatgta tcgatgaaac aggtatgact taccgggagt
       61 gattgtttct gagtgaacag aggccacttt cgcctgataa atgaaaaatg agcgattttt
      121 gcaagttagt ttttctatag ccgagtacgc tcagcgcctc gcttgattac aatgaggttg
      181 ctgctatgag tcctgataat atcggttcgg ctgtcaatcg cttctcaggt tggccatcta
      241 atgatcctct aaaaacagat gaagaatcaa tgacatcacc cgtgactgtg caagtaataa
      301 agatgattgc cgccatcctt gatccacatc ggatagtcgt tggaattgat acactttata
      361 caacgcagcg cgaaacattt acctcagaga gtcaacagct cattgaatca gcattgcaaa
      421 cacttcatgt gatgaattta aataacctgg aaacaaatcg cgatgaagaa ggtcactatc
      481 tggcttttga atataacgtt gacacctctg acagaataac cgtttctgct tataatgaaa
      541 aaaaacctgg ctgctatggc caatgtcttg taatacagga tgacgaaaca aacttattta
      601 acctgataaa aattaaaatt ttcgaagcca ggcataatta tgtcgctcaa ttgatgagcg
      661 atccggaatt tatgttcaaa tttagttata gcgctcaaca aaatagatta gaacctcatc
      721 ttctggttcc cacttatttc cctctaaaaa caaacgaacc ggttacgcaa gaggatatac
      781 tgttacttta tcgtttcttt aaaatggatg gtgattttaa tatattaaca tcagatgaat
      841 acatgagtat catgacacct ctgatgaagt gtgagcgcag catccacgac cacaacaggt
      901 atgtgacggg caaagatacc cttcttctgg actatcctcc ttctggtaac cagatatatt
      961 tccatatttt tcctgacgaa tcagcaacac tagtccttta tttttcaaac aatgacattg
     1021 aatgtttcgt ctttgaaaga gatattccga gccagtatcg ctttttcaaa atgtttacca
     1081 atctggcact ggtt
//
LOCUS       DAZKFU020000586         1088 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZKFU020000586 DAZKFU020000000
VERSION     DAZKFU020000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.596"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(459..1031)
                     /gene="hdeD"
                     /locus_tag="ACHOQQ_RS25805"
                     /old_locus_tag="pgaptmp_005161"
     CDS             complement(459..1031)
                     /gene="hdeD"
                     /locus_tag="ACHOQQ_RS25805"
                     /old_locus_tag="pgaptmp_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGU4473265.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          371 a          249 c          212 g          256 t
ORIGIN      
        1 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
       61 agtaaataac acattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
      121 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
      181 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
      241 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
      301 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
      361 atagtgcgca attgatctac aactccgcgt agcggagaga gtattaatcg gatcatagtc
      421 acatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
      481 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
      541 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
      601 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
      661 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
      721 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
      781 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
      841 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
      901 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
      961 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     1021 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     1081 ccaaaaac
//
LOCUS       DAZKFU020000587         1087 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZKFU020000587 DAZKFU020000000
VERSION     DAZKFU020000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.597"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(4..483)
                     /locus_tag="ACHOQQ_RS25810"
                     /old_locus_tag="pgaptmp_005162"
     CDS             complement(4..483)
                     /locus_tag="ACHOQQ_RS25810"
                     /old_locus_tag="pgaptmp_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473266.1"
                     /translation="MHSLLILLYVVVWRACIILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            complement(566..958)
                     /locus_tag="ACHOQQ_RS25815"
                     /old_locus_tag="pgaptmp_005163"
     CDS             complement(566..958)
                     /locus_tag="ACHOQQ_RS25815"
                     /old_locus_tag="pgaptmp_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473267.1"
                     /translation="MSTFILCPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
BASE COUNT          304 a          312 c          248 g          223 t
ORIGIN      
        1 ccgtcagtct gcgtcggaaa attcaataca ggcctgcttc aggagcccgg ctatacgttg
       61 attcagacat tccgtccagc catcgttcca tccacgctcc catggattgc agacatcctc
      121 ccatggattg cagacatcgt tacagccatt cttccatgtc ttccatgcct ccatctccat
      181 gacagccccc agcctgtggc ctttcacacg tatatgccac agaaacggcc ggaaaagtcc
      241 tgatttcagt tggtcaccac cgcttttgta gcccagatga cgtgcaatac tttcagtata
      301 ttcaaatgca tcattctgca ttccagccgc cttagccagc gagctgtacg catcacgaag
      361 tatcgtcata ccttccagtt gatactcaca ccacgcacgg acagccgaca ctggtgcgat
      421 tacccgaaga atgatgcagg cacgccatac aacaacgtaa aggagtatta acagactatg
      481 catgtgttca cctcagatac aaaaaataac gggaacacac ctgccctgcg ggaagcgtgt
      541 tcccgacagg gtaataaatg ataaatcagc agggctgtgc gacgattcca tcaccctgca
      601 gaccaaaccg gtaacgcagg gtggtcagtt gctccgccat acggcaggca gcatgccatg
      661 tcctgcgacg ggatatccgt ccggtaaacc ggttcagata aatgacctca cgcggtaacg
      721 gctgatacca tcccgtgtac tcgttgcgat acattccctg ccggtaatac aggcagggtg
      781 tccagacacc gccacgacgg aacatacaga cccgcacgct cagattgccg gccggattag
      841 tgtaccagcg actgtaatat gacaggttat tgcgggaaaa aaacacccgc aactgagccg
      901 tcattcgccg gaaaaaagaa gagatgcccg gttcagggca caaaataaac gttgacataa
      961 agactcctgt aaaaaaacgg gagccttcgc cccgacggac aaaaactccc gtcagggtta
     1021 aaacaccacc agtacagcat ggtgttgctg tcagaaaaag cccgttaata aagggcgcag
     1081 ttataaa
//
LOCUS       DAZKFU020000588         1078 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZKFU020000588 DAZKFU020000000
VERSION     DAZKFU020000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.598"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            291..>1078
                     /gene="iolR"
                     /locus_tag="ACHOQQ_RS25820"
                     /old_locus_tag="pgaptmp_005164"
     CDS             291..>1078
                     /gene="iolR"
                     /locus_tag="ACHOQQ_RS25820"
                     /old_locus_tag="pgaptmp_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator IolR"
                     /protein_id="HGU4473268.1"
                     /translation="MSKHQTQLSLLQDDIRSRYDSLSKRLKQVAQYILDNSNSVAFDT
                     VASIAQHAEVPPSTLIRFANAFGFSGFNEMKQVFRQHLMEETVSYTERARLFRQKNIN
                     DGEPTPEKPGEILKLFSMVNAQVLQQLPMQIKNEQLDAAVSLLSKADNIYVIGLRRSF
                     SVACYLTYALRHLERRAFLIDGIGGMFSEQLSMISSNDVVIAISYSPYAQEVVELVEL
                     GAKRDAHHIVITDSQVSPLAAFSEVCFVIREAQVDGFRSQVASM"
BASE COUNT          300 a          218 c          250 g          310 t
ORIGIN      
        1 gccacacacg tgtcagctct ttgtgacctg acgcaatcat ctgactcagc agcgattcac
       61 ggtactggtc tttttggtga ccgttctgac agagcaatac agtgggacaa acgaccatcg
      121 tcatcactgg agaggcgtgc atggaaactc gtcaacaact caagttcgcc ggaacggaac
      181 gttaacaata gaactttact gtataaattt gaatactgct aaatgtatga atggttcatt
      241 catgttaata ttgtgatttg caaaaatttc attttattag agtaaaatac atgtctaaac
      301 accaaacaca gctttcctta ctgcaggacg acatccgcag tcgctatgac agtctcagca
      361 aacgtcttaa acaagttgct cagtatattc tggataacag taatagcgtt gcttttgata
      421 cggttgcttc tattgcgcag cacgcggagg tacctccttc gacattaatt cgttttgcca
      481 atgcgtttgg ttttagcgga ttcaatgaaa tgaaacaagt atttcgccag catttgatgg
      541 aggaaaccgt tagctataca gaacgggctc gtctgttcag acaaaaaaac atcaatgatg
      601 gggaacccac accggaaaaa ccgggcgaaa ttctgaagct attttccatg gtaaatgcac
      661 aggtactaca acaattaccg atgcagatta aaaatgagca actggatgct gcagtaagtt
      721 tattgtcaaa agccgataat atttatgtga ttggattacg tcgttcattt agcgtggcct
      781 gttatttaac ctatgcattg cgccatcttg agaggcgtgc atttttaatt gatggtattg
      841 gaggcatgtt ttctgagcaa ctgagtatga tcagctctaa cgatgtagtc atcgccatca
      901 gttattcgcc atacgctcag gaggttgttg aactggttga gttgggggca aaacgtgatg
      961 cacatcatat cgtcattacc gacagtcagg tcagtccgtt ggctgcgttc agtgaggtct
     1021 gttttgtaat acgtgaagcg caggtggatg gcttccgttc gcaggtagcg tcgatgtg
//
LOCUS       DAZKFU020000589         1063 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZKFU020000589 DAZKFU020000000
VERSION     DAZKFU020000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.599"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1063)
                     /gene="espI"
                     /locus_tag="ACHOQQ_RS25825"
                     /old_locus_tag="pgaptmp_005165"
     CDS             complement(<1..>1063)
                     /gene="espI"
                     /locus_tag="ACHOQQ_RS25825"
                     /old_locus_tag="pgaptmp_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HGU4473269.1"
                     /translation="YTAFYRAGVGSQYIRYANGTDKLLQAYTPEKAYLTGGTVGKPYY
                     THYNGMKMISANPGNTFDKNQRPLASYGQSGDSGSPLYAWDNIDKKWVLAGVTLHNYG
                     VKGARNDWLLIPHDFISQKLQDDLKPIIVASPEENILRWEFDRSRGTGTLSQGEKIFS
                     MTGSVNGNANTGNNLVFSGNEGKIELVSSVEQGAGYLQFDKDYTVLTNNNSTWTGAGI
                     IVGDEANVKWGVNGIAGDNLHKVGSGTLTVNGHGENKGGLKVGDGVVVLEQQPDANQK
                     QQAFSHINIASGRATVKLNGANQVDADNISWGYRGGKLDLNGYDFTFSRLQAADYGAE
                     ISNDNQTDKSIVTLSLSPLK"
BASE COUNT          278 a          268 c          194 g          323 t
ORIGIN      
        1 ctttcagagg agataacgaa agtgtgacta tggatttatc tgtctgatta tcgttgctga
       61 tttcagcacc ataatctgca gcctgaagac gggaaaaggt aaaatcatac ccatttaaat
      121 ccagtttccc cccccgatat ccccagctga tattatctgc atctacctgg tttgccccgt
      181 taagtttaac tgttgcccga ccactggcta tattgatatg gctaaacgcc tgttgtttct
      241 ggtttgcatc tggctgttgc tcaagaacaa caacgccgtc cccaacttta aggccacctt
      301 tattctcacc atgaccatta acagttaatg tcccggaacc aactttatgc agattatcac
      361 cggcaatgcc attaactccc catttgacat ttgcctcgtc accgacaata attccggcac
      421 cagtccatgt actgttatta tttgtcagta cagtgtagtc tttatcaaac tgaagatatc
      481 cggctccctg ctccacactg gataccagtt cgattttccc ttcattacct gagaacacaa
      541 gattgttccc ggtatttgca tttccgttta cactaccagt catggaaaaa atcttctctc
      601 cctgactgag agtacctgta cctctcgaac gatcaaattc ccagcgtaag atattctcct
      661 ccggagaagc aacaataatt ggtttaaggt catcctgtaa tttttgactg ataaagtcat
      721 gaggtatcag gagccagtca tttcgtgcac cttttactcc ataattatgc agagtaactc
      781 cagctaatac ccattttttg tcaatgttat cccaggcata taacggcgaa ccactgtctc
      841 cactctgtcc ataactggca agaggtctct ggtttttatc aaaggtattt cccgggtttg
      901 ctgaaatcat cttcataccg ttatagtgag tataataagg tttccccact gttccgccgg
      961 tcagataagc cttttctgga gtgtaagcct gcagtagttt atctgtgcca tttgcataac
     1021 ggatatattg agagccaacg cccgcacggt aaaaagccgt ata
//
LOCUS       DAZKFU020000590         1043 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZKFU020000590 DAZKFU020000000
VERSION     DAZKFU020000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.600"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(52..519)
                     /locus_tag="ACHOQQ_RS25830"
                     /old_locus_tag="pgaptmp_005166"
     CDS             complement(52..519)
                     /locus_tag="ACHOQQ_RS25830"
                     /old_locus_tag="pgaptmp_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473270.1"
                     /translation="MLKINTLRDAMTDACPWCRANPEKFTVYVNNGGIETTGESPSYL
                     YRYTLGIFVMDFTQDLDELMVPLMRWLYRNQPDLLLNPEKNRDIKFSVAINDDDSTDI
                     LLEIPVWERVIIRRNDDGTVHAEHIGEPPVSRALEAWRVTLEDVTWGQNDGSG"
     gene            complement(535..708)
                     /locus_tag="ACHOQQ_RS25835"
                     /old_locus_tag="pgaptmp_005167"
     CDS             complement(535..708)
                     /locus_tag="ACHOQQ_RS25835"
                     /old_locus_tag="pgaptmp_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4473271.1"
                     /translation="MLFIHQIMLYFCTAVCALYLISGGYSVIRNYFQRKIDERAAEKI
                     SQKSAQQNNQNMP"
     gene            complement(855..>1043)
                     /locus_tag="ACHOQQ_RS25840"
                     /old_locus_tag="pgaptmp_005168"
     CDS             complement(855..>1043)
                     /locus_tag="ACHOQQ_RS25840"
                     /old_locus_tag="pgaptmp_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473272.1"
                     /translation="NNSISVTPVINGQTQAAFNLAKCPASGVENDKLLITDITSNQDT
                     YPVLIEYVTVDINNSNAA"
BASE COUNT          238 a          269 c          253 g          283 t
ORIGIN      
        1 gcggacagac cactgaggat ctcctgaaag agaccgtcca gtctgtgcag gtcatccgga
       61 gccatcattc tgaccccatg tgacatcttc gagggtgacg cgccatgctt ccagggcccg
      121 gctgacaggc ggctctccga tgtgttctgc gtgaacagtg ccatcatcat tacggcggat
      181 aatcacccgc tcccataccg ggatttccag caggatatcg gtgctgtcgt cgtcattgat
      241 ggcaacagaa aatttgatat ccctgttttt ctccggatta agcaggagat caggctgatt
      301 gcggtacagc cagcgcatca gcggcaccat cagttcatcg agatcctgcg tgaagtccat
      361 gacaaagatc ccaagcgtgt aacggtaaag atatgagggg gattcgccgg tcgtctcaat
      421 gcctccgtta ttcacataca cagtgaattt ctccgggttg gcgcgacacc atgggcaggc
      481 gtcagtcatg gcgtcgcgca gggtgttaat tttcagcatg ccccctcctg tgtgttatgg
      541 catgttctgg ttgttctgtt gtgcagactt ctgactgatt ttttcagcgg cgcgctcgtc
      601 aatttttcgc tgaaaataat ttctgataac actgtaaccg ccgctgatca gatacagcgc
      661 gcagacggca gtacagaaat acagcatgat ctgatgaatg aataacataa tcacctcaca
      721 caaatgattg acataacgat gtctgtcgtt ttaaaaaaaa tgaataacaa taataaacac
      781 tctccataat tcctcttgcg ctcccgtcag gtatcttctc ctctcagtct tgcctgccgg
      841 gagcctcttt tttcttatgc ggcgttgctg ttattgatat ccaccgtcac atactcaatc
      901 agcaccgggt aggtgtcctg gttactggtg atatccgtga tcagcagttt gtcgttttca
      961 acacctgatg ccgggcattt cgccagatta aaggccgcct gtgtctgacc gttgatgacc
     1021 ggtgtcacgc tgatgctgtt att
//
LOCUS       DAZKFU020000591         1022 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZKFU020000591 DAZKFU020000000
VERSION     DAZKFU020000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.601"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..757
                     /locus_tag="ACHOQQ_RS25845"
                     /old_locus_tag="pgaptmp_005169"
     CDS             <1..757
                     /locus_tag="ACHOQQ_RS25845"
                     /old_locus_tag="pgaptmp_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4473273.1"
                     /translation="SNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTS
                     LGTNLSWRTPIATIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAP
                     GLEGAHINGQKYNRTNSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYR
                     GAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSLLFIRVDEI
                     PTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            770..>1022
                     /locus_tag="ACHOQQ_RS25850"
                     /old_locus_tag="pgaptmp_005170"
     CDS             770..>1022
                     /locus_tag="ACHOQQ_RS25850"
                     /old_locus_tag="pgaptmp_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU4473274.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLLETTNAIKVHFLTQNALRFKVGS"
BASE COUNT          324 a          204 c          210 g          284 t
ORIGIN      
        1 atcgaattct acctcatttt ccaaagatgg ctattcctcc aacaatactg gaattactgg
       61 catagccggt gaacatgatc agttaaatta tggtatatat gttaatcagc aacaacaaaa
      121 taatgatacc tcgcttggta cgaatttaag ctggagaact ccaatcgcca caatagatgg
      181 cagctatagc cattctaaaa acgcctggca aagtggtgga agtattagta gtggattagt
      241 tgtctggccc ggtggtatta atatcactaa ccagctatcc gatacatttg caattctgga
      301 tgcgcctggg ttagaaggcg cgcatattaa tggacaaaaa tacaaccgaa caaacagcaa
      361 aggccaggtt gtttacgacc tgatgatacc tcatcgtgaa aaccatctgg tacttgatac
      421 agcaaacagt gaaagtgaaa cagaattgca gggcaatcgt caaattattg cgccttaccg
      481 tggagcagtt tcttatgtgc agtttacaac tgaccaacgt aagccctggt atatacaggc
      541 actgcgtccc gatggttcgc cattaacttt tggctatgac gtactggatc tccaggaaaa
      601 caatattgga gtcgttggcc agggtagtct cctttttatt cgcgtagatg aaattcccac
      661 tggcataaaa gttgctctca atgatgaaca gaatttattc tgtactatta cttttcaaca
      721 cgttatcgat gaaaacaaaa catatatatg ccagtaaaag gagtttacta tgaagtggct
      781 tttattaatc accttgagtc tatattcttt tattgtgcaa agtgctccct gtggattgac
      841 caatgtcggg gaacaacgtg gaacatatat actcaagcca ctgagtatga aaggtaatct
      901 caccgccgaa aaaattgaaa acaccggaaa tatcattgag tgtgcagact tgctagagac
      961 gactaacgcc attaaagttc attttttaac acagaacgct ttaaggttta aagtcggaag
     1021 tc
//
LOCUS       DAZKFU020000592         1021 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZKFU020000592 DAZKFU020000000
VERSION     DAZKFU020000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.602"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..436)
                     /locus_tag="ACHOQQ_RS25855"
                     /old_locus_tag="pgaptmp_005171"
     CDS             complement(<1..436)
                     /locus_tag="ACHOQQ_RS25855"
                     /old_locus_tag="pgaptmp_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473275.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQSVPGNSVNCMCWASLAALAFDAEERVE"
     gene            complement(672..881)
                     /locus_tag="ACHOQQ_RS25860"
                     /old_locus_tag="pgaptmp_005172"
     CDS             complement(672..881)
                     /locus_tag="ACHOQQ_RS25860"
                     /old_locus_tag="pgaptmp_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473276.1"
                     /translation="MCVGCGYSGVRDGAGKNALVWVKVNPESGLGLCSGCDSFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
BASE COUNT          267 a          316 c          249 g          189 t
ORIGIN      
        1 gttccacccg ctcttcagca tcaaatgcca gtgcagcaag gcttgcccag cacatgcagt
       61 tcactgaatt ccccggaacg ctctgcagct cccggcgcac catgttgacc tcagcactga
      121 aaaaagcgtt ctcatcgcct tcaagggcag ccagatgcag cggaagccag acctgactga
      181 accattcgca catccgttcc cggttactga cggtcatcac tgtctccggg cgggaaccgt
      241 cctgaaaaac atgtccggtg cgtggagaac tgtatgaaac ataaccaccg tgcaccgcaa
      301 tgaccggacg acctgacgcc ggaagcccca cacgacaccc actggcaatc cgggtcatca
      361 gcaggttcag ttcagccagt aattcagggc gggttaagac cgccggaacc ggagaatatt
      421 tcactgagga agacatactt tcacctccat atctctcagc ccgccaggtg cgtcacttcc
      481 ccgcagagaa ccggaaacct ggctatatga aaaaagggaa aagggtaccg gacgaacagt
      541 tgtcgggaat aaaatcccgg taactgtctg tacgttttca gaccggtaag tttggcgacc
      601 ctggtcagaa ggtacggaca gcatctttgc gctcataact gtaccgaccg ggatatgcat
      661 tacaaagact gtcataccgc cccctccgtc tttcgctgtt tgcaggaaag accacatgaa
      721 aaaagctggt catgccgctg acgacgctgg cgttcctgca aaagaaagga gtcacaaccg
      781 gaacacagcc ccagaccact ctcaggattc accttcaccc agaccagagc attcttaccg
      841 gcaccatccc gtaccccgga ataaccacag cccacgcaca ccgtccactt cttcagtacg
      901 atgagtgcag tttcgagtga tgcgcggaga cgccgggagg caataaggag caacggctca
      961 tggtcataaa aaaccccgcc gttaccccct ccggattcaa gcagcgggag tcctgcaggc
     1021 t
//
LOCUS       DAZKFU020000593         1019 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZKFU020000593 DAZKFU020000000
VERSION     DAZKFU020000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.603"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1019)
                     /gene="espX6"
                     /locus_tag="ACHOQQ_RS25865"
                     /old_locus_tag="pgaptmp_005173"
     CDS             complement(<1..>1019)
                     /gene="espX6"
                     /locus_tag="ACHOQQ_RS25865"
                     /old_locus_tag="pgaptmp_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HGU4473277.1"
                     /translation="QFIKIMEQKAQLSIYIDKNNLKESIFSVLHNDPQHVDKDQHLPC
                     EKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPVENQLTIRDNNNKTETFSFTNL
                     QWENLLQYYKENHQQPNIAGSRNLTDNIDKIKNTISTSEIIECASPEIGSSVLNDLYS
                     IANFLPDNNLTPNESWKRFCDTCERFYVAQKSITGDKSERLTRKLSISDAGITMTFKI
                     GDVVINTIITAIPEDATGQRCIEGLNLAEMDLTGIDLSKMVLRNVNFNGSILRHAKFS
                     GTICEGVDFTDCDLRNAKFKNASLENNDFRKVRHLTYVNFKNANLRNSNFSGKVLTGV
                     NFTGS"
BASE COUNT          285 a          180 c          192 g          362 t
ORIGIN      
        1 tacttccagt aaaattaacg ccagtgagaa cttttccgct gaagttactg tttcgtagat
       61 ttgcgttttt gaaatttaca taagtcaagt ggcgaacttt acgaaaatca ttattttcta
      121 atgaggcatt tttaaatttt gcattacgca gatcacaatc tgtaaaatcc acgccttcac
      181 agatcgtacc ggagaacttg gcatgtctaa gaatgctgcc attaaaattg acattcctta
      241 gaaccatttt agacaagtct atgccggtta aatccatttc tgctaaattc aacccttcga
      301 tacaccgttg acccgttgca tcttcaggaa tagcagtgat aatagtattg atgacaacat
      361 cacctatctt gaaggtcatt gtaattcctg catcagagat agagagtttt cgcgtaagac
      421 gttcactctt atctccagtg atactcttct gagcaacgta aaagcgctcg catgtgtcac
      481 aaaatctttt ccagctctca tttggggtca gattattgtc cgggaggaaa ttagcaatgc
      541 tataaagatc gttcaggacg ctacttccta tttcaggaga ggcgcattca ataatttcag
      601 aggtggatat tgtattttta attttatcta tattatccgt gagatttcgt gatcctgcta
      661 tatttggctg ctggtggttt tctttgtagt attgcagcaa attttcccat tgtaagtttg
      721 taaacgagaa agtttcagtc ttgttattgt tatcccgtat ggttaattga ttttctactg
      781 ggtcaaaaga aatcatgaag ttattcaggt gttgatattg atgagtggca tctaatttca
      841 cttcaaatga atttgaactt ttgcaggcat gttttaaaaa cttttcacag ggaaggtgtt
      901 gatctttatc tacgtgttgt gggtcattgt gcaaaacaga gaaaatactc tctttcaaat
      961 tgttcttgtc aatatagatt gatagttgcg ctttttgttc cattatttta ataaactgc
//
LOCUS       DAZKFU020000594         1018 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZKFU020000594 DAZKFU020000000
VERSION     DAZKFU020000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.604"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            233..>1018
                     /gene="reiD"
                     /locus_tag="ACHOQQ_RS25870"
                     /old_locus_tag="pgaptmp_005174"
     CDS             233..>1018
                     /gene="reiD"
                     /locus_tag="ACHOQQ_RS25870"
                     /old_locus_tag="pgaptmp_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inositol utilization transcriptional
                     regulator ReiD"
                     /protein_id="HGU4473278.1"
                     /translation="MAYVSQVSESNGPFVRFMHMHPRTVELILITEGKGEYFIGDRIY
                     PVRKGDLVIYNSEVVHDEYLESGRPVGTICCGINNIVCPGLRENALIPDDTVPVIPLY
                     HHYATIEKLMSSIFNVINQHTVEGPTMAQLLTRVLLKYVEGNVLMRTGNDITRRHKNL
                     LDSIKAYIDHNFYEPIRLDALATKFNVSPYYVSHEFKRRYGYSPMDYLIKRRLGEAQS
                     LLTTDEGGCEKITSIAYRVGFSNLSHFQNYFKNKVGKTPGQYRK"
BASE COUNT          305 a          172 c          226 g          315 t
ORIGIN      
        1 tatcaaagaa aataaattgt gattctgtat tgttttattc gatgatctta tccgtgttag
       61 atgagtttca ccagaaaata atatgatcta tgtaacgttt tattctgttt ttttatatta
      121 taaaaaaaat agttttagtt ttggccgtag cactacagat tcggataatg gaggaaaaat
      181 catgcgtttt tgcttctgtc cttcagaaca agataataag tcccgggctc agttggcgta
      241 tgtcagtcag gttagcgaaa gcaatggccc gtttgtacgg ttcatgcata tgcatcccag
      301 gaccgtggag ctaatcctta tcaccgaggg aaagggggag tatttcatcg gtgatcgcat
      361 ttacccagtc agaaaaggtg atttggttat ttataacagc gaagtagtgc atgatgagta
      421 tcttgaaagc ggacggcctg tgggtactat ttgctgtggg ataaacaata ttgtgtgccc
      481 gggtctgcgg gagaatgctc tgattccaga tgatactgtg cccgtaattc cactatatca
      541 tcattatgcg acgatcgaga aattgatgag ttcaatattt aatgttataa atcagcatac
      601 agtagaagga ccaacaatgg cgcagttgct gaccagggtg ctgcttaaat acgtcgaggg
      661 aaatgtatta atgcgcacag gtaatgatat aacacggcgc cataaaaatt tactggattc
      721 aataaaagct tatattgacc ataatttcta tgaacccatc cgtctggatg ctctggcgac
      781 taagtttaat gtcagccctt actatgtttc tcatgaattt aaacggcgct atggatattc
      841 gccaatggac tatttgatta aacgtcggtt aggggaggca caatctttac ttaccactga
      901 tgaaggtggg tgtgaaaaaa ttacctcaat tgcctatcgt gttggtttca gtaatttgag
      961 tcattttcag aactatttta aaaataaagt aggaaaaacg cctggacaat atcgtaaa
//
LOCUS       DAZKFU020000595         1015 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZKFU020000595 DAZKFU020000000
VERSION     DAZKFU020000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.605"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1015
                     /locus_tag="ACHOQQ_RS25875"
                     /old_locus_tag="pgaptmp_005175"
     rRNA            <1..>1015
                     /locus_tag="ACHOQQ_RS25875"
                     /old_locus_tag="pgaptmp_005175"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          244 a          230 c          317 g          224 t
ORIGIN      
        1 ataccagctg gctgcaactg tttattaaaa acacagcact gtgcaaacac gaaagtggac
       61 gtatacggtg tgacgcctgc ccggtgccgg aaggttaatt gatggggtca gcgcaagcga
      121 agctcttgat cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga
      181 aattccttgt cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc
      241 cacccgagac tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg
      301 gaaagacccc gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg
      361 ataggtggga ggctttgaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca
      421 ccctttaatg tttgatgttc taacgttgac ccgtgatccg ggttgcggac agtgtctggt
      481 gggtagtttg actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct
      541 aatcctggtc ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt
      601 gacggcgcga gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc
      661 catcgctcaa cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca
      721 tatcgacggc ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg
      781 tcccaagggt atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt
      841 gagacagttc ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt
      901 acgagaggac cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc
      961 cggtagctaa atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgc
//
LOCUS       DAZKFU020000596         1012 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZKFU020000596 DAZKFU020000000
VERSION     DAZKFU020000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.606"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1012
                     /locus_tag="ACHOQQ_RS25880"
                     /old_locus_tag="pgaptmp_005176"
     CDS             <1..>1012
                     /locus_tag="ACHOQQ_RS25880"
                     /old_locus_tag="pgaptmp_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HGU4473279.1"
                     /translation="NQDSDTLAALWPLYAKNNAQLMRDATADSLRQQLNAFVQLPPAS
                     DARTQGTQHAYDILKSYLMLARPDKADAGWLAKNALKALPKRRNVPDGIWQNVAPKLL
                     GFYAQNLPAHPEWKIKPDDELISTVRQILLKQIGQRNAESGLYQEMLKRVASNWPDLT
                     LADMTGDTDASTVFSTDEVVPGMFTRQAWEEQVQDAINEVVRTRRDEIDWVLTDKTHQ
                     TGSDISPEALKSRLTERYFTDFGNAWLNMLNSIQWHEPTSLSGAIAQLSLLGDVRQSP
                     LVALMNTLNYQGKTGQKGEALADSVVDSAKKLLDKNKNGRQFIEQTQGPEGPLDGVFG
                     PLT"
BASE COUNT          267 a          254 c          288 g          203 t
ORIGIN      
        1 aatcaggaca gcgacacgct ggccgcgctc tggccgctgt atgcgaaaaa taacgcacag
       61 ctgatgaggg atgccacagc agacagcctc aggcagcagc tgaatgcctt tgtgcaactc
      121 cctcctgcca gtgatgcccg tacgcagggc acacagcatg cttacgacat actcaaaagt
      181 tatctgatgc tggcgcgccc ggacaaagcg gacgccggct ggctggcaaa aaatgcgctg
      241 aaagccttac cgaaacgccg gaatgtgccg gatggcatat ggcagaacgt cgcaccgaaa
      301 ctgctggggt tttatgcgca aaaccttccg gctcatccgg aatggaaaat taagcctgac
      361 gatgaattaa tcagtacggt ccgtcagatt ctgcttaagc aaattggcca gcggaatgct
      421 gagtccgggt tatatcagga gatgcttaag cgcgtggcca gtaactggcc tgatttaaca
      481 cttgcggata tgaccggtga caccgatgcc tcaacggtat tcagcactga cgaagtcgtt
      541 ccgggaatgt ttacgcgtca ggcctgggaa gagcaggtac aggatgcgat taacgaagtt
      601 gtcagaacac gccgcgatga aattgactgg gtactgacgg ataaaacaca tcagacaggg
      661 agcgatatct ctccggaggc cctgaagagc cgcctgactg aacgctattt taccgatttt
      721 ggtaatgcct ggctgaacat gctcaacagc atacagtggc atgaacccac gtcactttcc
      781 ggagccattg cccagttgag tctgctcggt gacgtccgcc agtctccgtt agttgcactg
      841 atgaacacgc tgaactacca ggggaaaacg gggcagaagg gggaggcact ggctgattca
      901 gtcgtggatt cagcgaaaaa gctgctcgat aagaataaaa atggcaggca gtttattgaa
      961 cagacacagg gccctgaagg tccgctcgat ggggtatttg gaccgctgac ag
//
LOCUS       DAZKFU020000597         1012 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZKFU020000597 DAZKFU020000000
VERSION     DAZKFU020000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.607"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..249
                     /locus_tag="ACHOQQ_RS25885"
                     /old_locus_tag="pgaptmp_005177"
     CDS             <1..249
                     /locus_tag="ACHOQQ_RS25885"
                     /old_locus_tag="pgaptmp_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4473280.1"
                     /translation="GDGSEESISLTFRNMNDFTPEQIARQIPRLKAMLAMRSLLRDLK
                     ANLLDNATFRKELEKILRDPELSQALRDELSALVPEQV"
     gene            270..>1012
                     /locus_tag="ACHOQQ_RS25890"
                     /old_locus_tag="pgaptmp_005178"
     CDS             270..>1012
                     /locus_tag="ACHOQQ_RS25890"
                     /old_locus_tag="pgaptmp_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU4473281.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKW"
BASE COUNT          250 a          226 c          296 g          240 t
ORIGIN      
        1 ggtgatggct ctgaagaaag catcagcctg actttccgca atatgaacga cttcacgcca
       61 gagcagatcg cccgacaaat tccccgtctt aaagccatgc tggccatgcg cagccttctg
      121 cgggatctga aggcgaatct gctggataac gccactttcc gtaaagaact ggaaaaaatt
      181 ctgcgggatc ccgaactgag tcaggcctta cgtgacgaac tgagtgcact ggtgccggaa
      241 caggtgtgat taacggatta agagagagca tgctgatgtc tgtacaaaaa gaaaataatg
      301 tggcgggaaa tgcggtttct gaaacgcatt ccggcgacag cgtatatgct tccctgtttg
      361 aaaaaattaa tctgaatccg gtatcagccc tgagtgcgct ggatatctgg caggatccgc
      421 aggccatgtc agaggcatca gcagatgaac gtctgaccgc agggatgcag gtgtttatgg
      481 aatgcctggc gaaagcggga gcaccggtgg agaaactcga taaggcgctg attgaccatc
      541 atattgcaga gcttgattat cagatcagcc gtcagctgga tgccgtgctt catcatccgg
      601 aatttcagaa agtggaatcc ctgtggcggg gcgtgaagtc tctggtggat aaaacggact
      661 tccgccgtaa tgtgaaaatt gagctgctgg atctgtccaa agacgatctg cgccaggatt
      721 ttgaggatgc gccggaaatt attcagagtg gtctttatct gcagacttat gtggcggagt
      781 acgatacgcc gggtggtgag cccattgcag ccctgatttc tgcctgggag tttgatgcgt
      841 ctgcgcagga tgtggcgctg ctgaaaaata tttcccgggt ggctgcgtcc gcgcatatgc
      901 catttattgg ttcagttggc ccggctttct tccggaagga tacgatggaa gaggtggccg
      961 ccattaagga tattggcaat cactttgagc gtgccgagta catcaagtgg aa
//
LOCUS       DAZKFU020000598          998 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZKFU020000598 DAZKFU020000000
VERSION     DAZKFU020000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.608"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..718)
                     /locus_tag="ACHOQQ_RS25895"
                     /old_locus_tag="pgaptmp_005179"
     CDS             complement(<1..718)
                     /locus_tag="ACHOQQ_RS25895"
                     /old_locus_tag="pgaptmp_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HGU4473282.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSAPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLYEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTSVYPNLLIWPAAMLLG"
     gene            complement(711..>998)
                     /locus_tag="ACHOQQ_RS25900"
                     /old_locus_tag="pgaptmp_005180"
     CDS             complement(711..>998)
                     /locus_tag="ACHOQQ_RS25900"
                     /old_locus_tag="pgaptmp_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative coupling factor TraD, PFGI-1 class"
                     /protein_id="HGU4473283.1"
                     /translation="QDFTSSTQDRVGTVKVPLLEPADIVTLPKGQAFALLEGGQLWKI
                     RMPLPATDADDALMPESIAKIAEAMRRNYHTGEGWWNETPSVNMPEGEQNG"
BASE COUNT          243 a          322 c          231 g          202 t
ORIGIN      
        1 cccccagcag catggccgcc ggccatatca gcaggttcgg atacaccgac gtcggccagg
       61 acaagtaaag aatgcaggga atatatatcg ccggtttaat agactttttt gcatggtgat
      121 aaacaaagct ggactcatac gcagcaccat accggcgcag atcacgacgc cccagtccct
      181 ccacaaccgc gatcagtatc accatcacaa acagcggaac agacaacacc aggatcgtca
      241 cccgtatcag tgtgaccacc gtgacatata ctgtggccat caggtactca tacagataat
      301 ggctcagcca gcgccccaca tcctcactgt actgtgacac actgttaccc accacggcac
      361 cggcattttg ctgcttcagc cacgacagaa aacctgtttc cacaaacagc cactgatacg
      421 ccgttgctat ccattctgtc accgttaccg atggtgccga cagcagcaga ctgcgggtga
      481 attccgaaga cagccacccc agttctgtct tcatcactgc ctcactgtgt gctgccccct
      541 gctccggcca gaaaaaagca atcccgatat actcaaccag caggctcagc agcagggagg
      601 aaaacaggat cccgaccagc atccacggcc agccccacaa cagattccag agcagtccgt
      661 gctgacgtgg cggagagggt tgtgctgctc tgttcacctc acgacggggt tcagccattc
      721 tgctctcctt ccggcatgtt cacggaggga gtctcattcc accatccctc tccggtgtgg
      781 taattacgcc gcatggcctc tgcaattttt gcaatactct ccggcatcag cgcgtcatct
      841 gcatcagttg caggcagggg catacggatt ttccacaact gtcccccttc cagcagagca
      901 aaggcctgtc ctttcggcag ggtcacgata tctgccggct ccagcaaagg caccttcaca
      961 gtaccaacac gatcctgggt actggatgtg aagtcctg
//
LOCUS       DAZKFU020000599          996 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZKFU020000599 DAZKFU020000000
VERSION     DAZKFU020000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.609"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            82..405
                     /locus_tag="ACHOQQ_RS25905"
                     /old_locus_tag="pgaptmp_005181"
     CDS             82..405
                     /locus_tag="ACHOQQ_RS25905"
                     /old_locus_tag="pgaptmp_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HGU4473284.1"
                     /translation="MAYHSPDVTLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMHEQMSRIIRKTGTDEILFRLSKHHHLYEKK
                     KTKEG"
     gene            complement(793..>996)
                     /gene="subB"
                     /locus_tag="ACHOQQ_RS25910"
                     /old_locus_tag="pgaptmp_005182"
     CDS             complement(793..>996)
                     /gene="subB"
                     /locus_tag="ACHOQQ_RS25910"
                     /old_locus_tag="pgaptmp_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HGU4473285.1"
                     /translation="SVWGPSFSTLYNQALYFYTTGQLVRIYYEPGVWTYPPFVKALTS
                     NALVGLSTCATSTECFGPDRKKN"
BASE COUNT          280 a          235 c          241 g          240 t
ORIGIN      
        1 gtgggagaat tcgctctccg gggcgtctgc gtccccggcg gagaataaac cggagatgat
       61 gattctccac aggagtattc aatggcatac cattccccgg atgtcacact gcattatccg
      121 tatctgcgcc ggatgtcctg ggcgcagata acggcagcag ctgaagatgc ggaaatacat
      181 catgattatg cccgggcatt aatattgtgg cagcatgcat atcatgctgc cacgctgact
      241 ctcaacaaaa acctggcagt tgctaaaatc gacttctgta ttaaacgtat ccggatgcat
      301 gaacaaatga gcaggataat ccggaagact ggcacagacg aaatactctt ccgtctgagt
      361 aaacaccatc atttatatga gaaaaagaaa acaaaagagg ggtgagaaac ccattgtatg
      421 gggaaaactt gccatgctca ctgcgggggc atttttgtac tggctgttct aaccgggtaa
      481 cccgttgata cacatagtac aataatgttt tatgcaggct tacgcgccag actgccatca
      541 gtagtgcatt gcgtgccagc tcgcggacag gggcagtgac cagccaccac atgagtaagc
      601 atcatttcag cgccatcagg cagctcctga aactcctgcg agttgaaaaa gcatcctcct
      661 gcattagcaa tactggcttg tcagtgccag tgtgatataa gacgtaatgt caaagttcgt
      721 ggtgttgaca tatttctgtc gcaaccaaag tatgctcccg cccagtatat atctgggcag
      781 gagcatcatt tattagttct ttttcctgtc ggggccaaaa cattctgtcg atgtggcgca
      841 ggttgataac ccaacaaggg cattggacgt taatgccttc acaaagggcg gatacgtcca
      901 taccccgggt tcataataaa tcctgaccaa ctgacctgtt gtgtaaaaat atagtgcctg
      961 attatataat gtggaaaacg aaggccccca gacaga
//
LOCUS       DAZKFU020000600          996 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZKFU020000600 DAZKFU020000000
VERSION     DAZKFU020000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.610"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..473)
                     /locus_tag="ACHOQQ_RS25915"
                     /old_locus_tag="pgaptmp_005183"
     CDS             complement(<1..473)
                     /locus_tag="ACHOQQ_RS25915"
                     /old_locus_tag="pgaptmp_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473286.1"
                     /translation="MVMVLTGAGMGSAATGGVGMAGASTGTAVFPGAGSRDGDWEEDN
                     ISYPGSGSGSRQGNGGFYYQGKQYDPILNEPMVVSAETHGDFWEDDMDHSETVNYEGI
                     DSKAEVQAKTDEKTCENCPPEGKVMPMIRRCSRWSMVTISYQTRICGTFYNPETQ"
     gene            complement(473..817)
                     /locus_tag="ACHOQQ_RS25920"
                     /old_locus_tag="pgaptmp_005184"
     CDS             complement(473..817)
                     /locus_tag="ACHOQQ_RS25920"
                     /old_locus_tag="pgaptmp_005184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473287.1"
                     /translation="MLKLSEEQCVGMDKREDDDFVCDTALRLKKKFPGITESDDTFHV
                     RLRKALDYANTLPLKEKNVKRDFLFLEAFWPGFYLKPEVDKWLRTPNAYSTDQRVEDY
                     KHVMINRERREL"
     gene            complement(811..>996)
                     /locus_tag="ACHOQQ_RS25925"
                     /old_locus_tag="pgaptmp_005185"
     CDS             complement(811..>996)
                     /locus_tag="ACHOQQ_RS25925"
                     /old_locus_tag="pgaptmp_005185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473288.1"
                     /translation="MNTELPDGRIALLRFHDPRVQKRLGEMLNDQQHRELTGLMQEWL
                     TIVDGKAWSFKQREFIC"
BASE COUNT          226 a          300 c          180 g          290 t
ORIGIN      
        1 tgttgcgtct ccggattata aaacgtgcca catattcttg tctggtagct gattgttacc
       61 atactccagc gcgagcaacg ccttatcatc ggcattacct tcccttccgg tgggcagttc
      121 tcacacgttt tctcatccgt cttcgcctgt acttcggctt tgctatcaat accttcatag
      181 tttactgttt ctgaatgatc catatcatct tcccagaaat caccatgcgt ctctgcgctg
      241 acaaccatcg gctcattcag aattggatca tactgtttcc cctgataata aaaccctccg
      301 ttaccctgac gcgaaccact gcctgagcca ggataagata tattatcttc ttcccagtct
      361 ccatcgcggc ttcctgctcc gggaaatact gctgttccgg ttgaggcacc agccatacca
      421 acacctccgg tagctgcgct ccccatacca gcccctgtca ggaccataac catcagagct
      481 ccctcctttc tctgtttatc atcacatgct tataatcttc aacccgctgg tcagttgaat
      541 aagcattcgg cgttctcaac catttatcca cttcgggttt cagataaaaa ccaggccaga
      601 aggcctccag aaacaagaag tcccgcttca cgtttttctc tttaagcgga agggtattgg
      661 cataatccag cgcttttctc aggcgaacat ggaacgtgtc atctgattca gtaattcccg
      721 gaaatttctt cttcagtctt agcgcggtgt cgcaaacaaa atcatcatct tcccttttgt
      781 ccataccaac acactgctct tcagacaact tcagcatatg aactcccttt gtttaaatga
      841 ccaggctttt ccgtccacta ttgtcagcca ctcctgcatt aaccccgtca gctcccgatg
      901 ttgttggtcg ttcagcattt ctcccaggcg cttctgaacg cgcggatcat ggaaacgcag
      961 caacgcaatc cgtccatccg gtagttctgt attcat
//
LOCUS       DAZKFU020000601          992 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZKFU020000601 DAZKFU020000000
VERSION     DAZKFU020000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.611"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            103..345
                     /locus_tag="ACHOQQ_RS25930"
                     /old_locus_tag="pgaptmp_005186"
     CDS             103..345
                     /locus_tag="ACHOQQ_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473289.1"
                     /translation="MMMARDITAYHLNSVHISARRFPEIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
     gene            771..992
                     /locus_tag="ACHOQQ_RS25935"
                     /old_locus_tag="pgaptmp_005187"
                     /pseudo
     CDS             771..992
                     /locus_tag="ACHOQQ_RS25935"
                     /old_locus_tag="pgaptmp_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
BASE COUNT          256 a          196 c          231 g          309 t
ORIGIN      
        1 agcagtaact ggctatcgtg tataattatc cagaagtcct gctgagcctt ccgtctgttc
       61 atcatgtgcc aggtttgcgt tcgggatatg atgtgccatc ttatgatgat ggcaagagat
      121 attactgcgt accacctcaa ttctgtacac atcagtgccc gtcgtttccc cgagattttt
      181 cagtgggcca ccggtatgca aacaaaaaat ctgccgttaa ctaattcttt gttacagaat
      241 gattacgcag aaagtatttt tgtatttaat ggcgaacgtg acgtttcgga aatgccttta
      301 ccggaagaaa tgctactgac tggaaacgct gagtcagaaa gataattcgg tgtattcatg
      361 actgttatca atgtgttatg tgtatgatgt caacgacgga tgaaaagtta tccacttata
      421 tgaggcatta taggattatt tctggctgac gttgtttcag ttcagaccag gggcagtgaa
      481 tatgctgacc tgctttttcg ctgatatcag tccccctgta attacataga taatagatat
      541 acctctccgt tgatcaggct gtccaggtaa ttgataccgc acttcctcat cagttcgtag
      601 cagagtgtac ttagcatggc tagtgcagtt tctgatgatt tacagtgtgt attcaggaga
      661 gtaagaaatt gctcaaaaca tcctggttta tactggtgct ttgatattca ttgtgtccct
      721 ttttcgttcg ttattatttc catatttaat atgatgcctg tacatgtaaa attggtgttc
      781 aggaggtcta taatgaaatc cggcagggga ctcccgtcag tccggtcata atggctggtg
      841 atgcctggct cacgacaaaa attcgttctc agttacttac aagtgcccgt gtcaaatcat
      901 ccagtgtcaa ggtggtgaca gaaaacagcg aagtcttttt actgggcctg gttacagagc
      961 aggaagggcg agcggcagca gatgttgcca gt
//
LOCUS       DAZKFU020000602          992 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZKFU020000602 DAZKFU020000000
VERSION     DAZKFU020000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.612"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>992
                     /locus_tag="ACHOQQ_RS25940"
                     /old_locus_tag="pgaptmp_005188"
     CDS             <1..>992
                     /locus_tag="ACHOQQ_RS25940"
                     /old_locus_tag="pgaptmp_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU4473290.1"
                     /translation="EAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESWYLRSENAY
                     RAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARG
                     ATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDA
                     GSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGRGWLGSLETGLPFSIT
                     DNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHSSAQHVRVGFRLGSHNDMTFGEGTSS
                     RAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGT"
BASE COUNT          208 a          278 c          305 g          201 t
ORIGIN      
        1 gaagccatta acggtgccac cacggaggaa ggggcctttg tccaggggaa caggctgcag
       61 gccggtgcct ttaactactc cctcaaccgg gacagtgatg agagctggta tctgcgcagt
      121 gaaaatgctt atcgtgcaga agtccccctg tatgcctcca tgctgacaca ggcaatggac
      181 tatgaccgga ttgtggcagg ctcccgcagc catcagaccg gtgtaaatgg tgaaaacaac
      241 agcgtccgtc tcagcattca gggcggtcat ctcggtcacg ataacaatgg cggtattgcc
      301 cgtggggcca cgccggaaag cagcggcagc tatggattcg tccgtctgga gggtgacctg
      361 atgagaacag aggttgccgg tatgtctgtg accgcggggg tatatggtgc tgctggccat
      421 tcttccgttg atgttaagga tgatgacggc tcccgcgccg gcactgtccg ggatgatgcc
      481 ggcagcctgg gcggatatat gaacctgaca cacacctcct ccggcctgtg ggctgacatt
      541 gtggcacagg gaacccgcca cagtatgaaa gcgtcatcgg gcaataacga cttccgcgca
      601 cggggccggg gctggctggg ctcactggaa accggtctgc ccttcagtat cactgacaac
      661 ctgatgctgg agccacaact gcagtacacc tggcagggac tttccctgga tgacggccag
      721 gataacgccg gttatgtgaa gttcgggcat agcagtgcac aacatgtgcg tgtcggtttc
      781 cgtctgggca gccacaacga tatgaccttt ggtgaaggca cctcatcccg tgcccccctg
      841 cgtgacagtg caaaacacag tgtgcgtgaa ctgccggtga actggtgggt acagccttcc
      901 gttatccgca ccttcagctc ccggggtgat atgcgtgtgg ggacctccac cgcaggcagc
      961 gggatgacgt tctctccctc acagaatggc ac
//
LOCUS       DAZKFU020000603          984 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZKFU020000603 DAZKFU020000000
VERSION     DAZKFU020000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.613"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..>984
                     /locus_tag="ACHOQQ_RS25945"
                     /old_locus_tag="pgaptmp_005189"
     CDS             88..>984
                     /locus_tag="ACHOQQ_RS25945"
                     /old_locus_tag="pgaptmp_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGU4473291.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAG"
BASE COUNT          201 a          228 c          282 g          273 t
ORIGIN      
        1 caccacaacc catttaaatg aatttgataa tcattctcgt ttggcatagc atgaaacata
       61 gcaaaggcta tgttttagag gcacaagatg acgaactatc gcgttgagag tagcagcgga
      121 cgggcggcgc gcaagatgag gctcgcatta atgggacctg cgttcattgc ggcgattggt
      181 tatatcgatc ccggtaactt tgcgaccaat attcaggcgg gtgccagctt cggctatcag
      241 ctactgtggg ttgtcgtttg ggccaacctg atggcgatgc tgattcagat cctctctgcc
      301 aaactaggga ttgccaccgg taaaaatctg gcggagcaga ttcgcgatca ctatccgcgt
      361 cccgtagtgt ggttctattg ggttcaggca gaaattattg cgatggcaac cgacctggcg
      421 gaatttattg gtgcggcgat cggttttaaa ctcattcttg gtgtttcgtt gttgcagggc
      481 gcggtgctga cggggatcgc gactttcctg attttaatgc tgcaacgtcg cgggcaaaaa
      541 ccgctggaga aagtgattgg cgggttactg ttgtttgttg ccgcggctta cattgtcgag
      601 ttgattttct cccagcctaa cctggcgcag ctgggtaaag gaatggtgat cccgagttta
      661 cctacttcgg aggcggtctt cctggcagca ggcgtgttag gggcgacgat tatgccgcat
      721 gtgatttatt tgcactcttc gctcactcag catttacatg gcggttcgcg tcaacaacgt
      781 tattccgcca ccaaatggga tgtggctatc gccatgacga ttgccggttt tgtcaatctg
      841 gcgatgatgg ctacagctgc ggcggcgttc cacttttctg gtcatactgg tgttgccgat
      901 cttgatgagg cttatctgac gctgcaaccg ctgttaagcc atgctgcggc aacggtcttt
      961 ggattaagcc tggttgctgc cgga
//
LOCUS       DAZKFU020000604          969 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZKFU020000604 DAZKFU020000000
VERSION     DAZKFU020000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.614"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..812
                     /gene="yeeO"
                     /locus_tag="ACHOQQ_RS25950"
                     /old_locus_tag="pgaptmp_005190"
     CDS             <1..812
                     /gene="yeeO"
                     /locus_tag="ACHOQQ_RS25950"
                     /old_locus_tag="pgaptmp_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU4473292.1"
                     /translation="GLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIW
                     EVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSA
                     STIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKH
                     VVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIML
                     GWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
BASE COUNT          197 a          193 c          277 g          302 t
ORIGIN      
        1 ctgggttggg tttaaccatt tctcgttata ttggcgcagt tgcaattttg tgggtgctgg
       61 cgattggttt taatcctgcg ctaaggattt cgttaaagag ctattttaaa ccgctgaatt
      121 ttagcattat ctgggaagtc atggggattg gtattccagc gagtgtcgaa tcagtgttat
      181 ttaccagtgg tcggttatta acccaaatgt tcgttgccgg gatggggacc agtgttattg
      241 ccggaaattt tatcgcgttt tcaattgcgg ctcttatcaa cttacccgga agtgcgctcg
      301 gctctgcttc tacgatcatt acaggccgaa ggttgggggt agggcagata gcgcaagcag
      361 agattcagtt gcggcatgtg ttctggctgt ccactcttgg attaacggcc atcgcctggc
      421 taacggctcc ctttgccggg gttatggcat cgttttacac ccaggatcca caggttaaac
      481 atgtcgttgt gattctgatt tggctaaatg ctttatttat gcctatttgg tccgcctcat
      541 gggtgctacc cgctggattt aaaggtgctc gtgatgcccg ttacgccatg tgggtttcga
      601 tgttgagcat gtggggttgt cgggttgtcg tcggttatgt gctgggaatc atgcttggct
      661 ggggtgtggt tggtgtctgg atgggaatgt ttgccgactg ggctgtgcgg gccgtgctgt
      721 tttactggcg aatggttact ggacgttggc tatggaaata ccctcgatcc gagccgcaaa
      781 agtgtgaaaa aaagccagct gtgtcggaat aaacgacaaa atgcagatta tttcagcaaa
      841 cgatttcaaa tttaaaaaac aggctttgac attgtgggtg ggcatcgcta atattcgcct
      901 cgttctcacg attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc
      961 actggttcg
//
LOCUS       DAZKFU020000605          965 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZKFU020000605 DAZKFU020000000
VERSION     DAZKFU020000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.615"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..359)
                     /locus_tag="ACHOQQ_RS25955"
                     /old_locus_tag="pgaptmp_005191"
     CDS             complement(<1..359)
                     /locus_tag="ACHOQQ_RS25955"
                     /old_locus_tag="pgaptmp_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HGU4473293.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVIS"
     gene            complement(366..707)
                     /locus_tag="ACHOQQ_RS25960"
                     /old_locus_tag="pgaptmp_005192"
     CDS             complement(366..707)
                     /locus_tag="ACHOQQ_RS25960"
                     /old_locus_tag="pgaptmp_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473294.1"
                     /translation="MSRIYGRVKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            complement(712..>965)
                     /locus_tag="ACHOQQ_RS25965"
                     /old_locus_tag="pgaptmp_005193"
     CDS             complement(712..>965)
                     /locus_tag="ACHOQQ_RS25965"
                     /old_locus_tag="pgaptmp_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HGU4473295.1"
                     /translation="TGMAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLAR
                     NSVLTIIERDNERVGNNPMIQQADNADSRIHSLDAPDKE"
BASE COUNT          237 a          317 c          201 g          210 t
ORIGIN      
        1 ctgctgatca ccccactgta ccccgtctct ccgggctctt caggtgtcca taccccacag
       61 gtctgtgccc tggatttatc gaatgacagc gtatccagcg tcacctgtac cagaggaaca
      121 cagcaggata tcatcacaaa aaaagcacca tagagaacat gctcaatacg cggcagggaa
      181 agcatccctt tgtttccctc atccgcacct tcttccctga ctttcagcca gatcccgatg
      241 atcctgaatg ccagtggcag ggcaaacagt cctgtgctga tcaacacatt ccacagaccg
      301 ttattcacca cccagcccag cagagtcaga aaatattcca gatagttatt tgtggtcatg
      361 attgctcaac gccccggctc agcaaaaatg gcgtattcac acagcaggat aaacagaaca
      421 ctgactgtgg ccatacgcag aaaagaagcc cggtattctg ccctgaaccc cggcgcatgc
      481 cagatcctcc accccagcca tccgatcgca ctgtaaagac acagacgcca tcccagccag
      541 ccataacgtg tctcatacat ccactcccgc agggccgcaa atccttctgc gtgacgtata
      601 ccggtatcgg ccagccagag ggaaaacacg gtaatccctg tcagcaacag cgtatacagg
      661 gcgaatacct tcatcctctt tttcacccgg ccataaattc tgctcatcct ctcactcctt
      721 atccggggca tcgagagaat ggatacggct gtcagcattg tcagcctgct ggatcatcgg
      781 gttattcccc acacgctcgt tatcccgttc aatgatggtc agaacactgt tacgcgccag
      841 atcccgcttc agctccatct cgtttttcag cgccataatt tcccgatcca gcgcctcaat
      901 acggcgttcc ccctcttcca gtgcagcagg ctgagctgca gcatacggct cggccatccc
      961 cgtga
//
LOCUS       DAZKFU020000606          960 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZKFU020000606 DAZKFU020000000
VERSION     DAZKFU020000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.616"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            119..>960
                     /locus_tag="ACHOQQ_RS25970"
                     /old_locus_tag="pgaptmp_005194"
     CDS             119..>960
                     /locus_tag="ACHOQQ_RS25970"
                     /old_locus_tag="pgaptmp_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4473296.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYADQFIHHDEYKHAVEIKDGEVK
                     DIDNALVITTGNGSYGVSVAGEGSNLAINNGVIVTTGGLYPSDNSSLSTALTASAIVS
                     EFGGAVILQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFSDGDNTFI
                     VTAGNHAVGVLACASPGSARACVSSLDEESTADTGNNENSAKAILDMAKGEITTHGTE
                     SYAAYANGTVVKSGDKLDYTNASVTLTNVDITTHGDNAHAIAARQGTVSFNQGEIYTT
                     GPDAA"
BASE COUNT          275 a          183 c          224 g          278 t
ORIGIN      
        1 ttatctattt aaatttcttg tgcttttgtt tttgtcttta ccctccgttt tggtaatctg
       61 aactcatagg ttatttgacc aggaatgttt gttaatccaa tgaatggatg gttaatctat
      121 gaaactgaaa aaactccccg ggtttagttt aggacttatt gctctggcgg tgggtaatgc
      181 atatgctgat cagtttattc atcacgatga gtataaacat gccgtggaaa taaaagatgg
      241 agaagtaaaa gatattgata atgcgctagt tataacaacg ggtaatggaa gttacggtgt
      301 atctgttgca ggagagggta gcaatttagc cattaacaat ggcgtcattg taacaacggg
      361 cggtttatat ccttctgaca atagttctct aagcacagca ttaacagcta gcgcaatcgt
      421 aagtgaattt ggcggtgctg ttattttgca gggtaaaaac acgattgtca caacggggga
      481 ttactcaatt ggtttgctaa gtcaaacgag tggaaacctg aatactgata ccataataag
      541 agtcaactct gacggttcgg ttaccccctc tttttctgac ggagataata cttttattgt
      601 tactgcgggt aatcacgcag tcggcgttct tgcgtgtgca tctcccggaa gcgctcgtgc
      661 gtgtgtatct tctcttgatg aagaaagtac tgccgataca ggaaataatg aaaatagcgc
      721 caaagcaata ctggatatgg caaaagggga gataacaacc cacggaacag aaagttatgc
      781 cgcttacgct aatggcactg tcgtcaagtc tggtgacaag cttgattata ccaatgccag
      841 cgttacatta accaatgttg atatcaccac gcatggggac aatgctcatg ccatcgctgc
      901 tcgccagggg acagtttcat ttaaccaggg agaaatttat acaacgggtc ctgacgccgc
//
LOCUS       DAZKFU020000607          944 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZKFU020000607 DAZKFU020000000
VERSION     DAZKFU020000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.617"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>944
                     /gene="traD"
                     /locus_tag="ACHOQQ_RS25975"
                     /old_locus_tag="pgaptmp_005195"
     CDS             <1..>944
                     /gene="traD"
                     /locus_tag="ACHOQQ_RS25975"
                     /old_locus_tag="pgaptmp_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Members of this protein family are the putative
                     conjugative coupling factor, TraD, as the term is used for
                     the SXT and TOL plasmid systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer system coupling protein
                     TraD"
                     /protein_id="HGU4473297.1"
                     /translation="ASRVAGQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLI
                     MRYVNNIADLYIRYAGKVISEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAI
                     EVALSSEAGKKLYDPILDGLRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDY
                     LDMSDTRPVFDWEQVIRKKAVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGM
                     NAGLPGGNGTKNPVNLHCDEFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTG
                     SAAKTAQVQGNFNNMIMLRVRENRTAELLTTQLSQVEI"
BASE COUNT          222 a          207 c          288 g          227 t
ORIGIN      
        1 gcttcccgtg tggccggtca gttatccggt gaaggtaaca gtgcggcctt ccgtgagttt
       61 gcatggcggt ttgtcaacat tgttgcccgt gcactggtgg cactggggga gcgcccggat
      121 tacacgctga ttatgcgtta tgtgaataat attgccgacc tttatatccg ttatgctggt
      181 aaggtgatca gcgaacagat gccggaactg gaaaacgcca ttctgaataa catgaatgta
      241 ctgggggaag cggatgttcc ccggaccatg cagaaccagc cggatgctgt gcgtatctgg
      301 gccattgagg tggctctcag cagtgaagcc ggaaagaaac tctatgatcc gatactggat
      361 ggtctgcgaa gcgcggtacg ttatgatcgt acctattttg acaaaattgt ggcatccctg
      421 ttgccgttgc tggaaaaact gaccacagga aagacggcag aactgctggc acctgactac
      481 ctggatatga gcgatacccg tcccgtgttt gactgggagc aggtgatacg taaaaaggcg
      541 gtggtgtaca ttggtcttga tgcgctgagt gacagtgagg tggccagtgc cgtgggtaac
      601 tccatgtttg cggatctggt gagtgtcgcc gggcatatct acaaacacgg aatgaatgcc
      661 ggtcttccgg gagggaatgg taccaaaaat ccggttaacc tgcattgtga tgagtttaat
      721 gaactgatgg gcgatgagtt cattccgtta atcaacaagg gaggcggtgc cggtatgcag
      781 gtgacggctt acacgcagac ctcttctgat attgaggccc gtaccggtag tgctgccaaa
      841 acggcccagg tgcagggtaa ctttaataac atgattatgc tgcgggtcag ggagaaccgg
      901 acggccgagt tactgacgac ccagttgtcc caggtggaaa ttta
//
LOCUS       DAZKFU020000608          933 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZKFU020000608 DAZKFU020000000
VERSION     DAZKFU020000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.618"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..107)
                     /locus_tag="ACHOQQ_RS25980"
                     /old_locus_tag="pgaptmp_005196"
     CDS             complement(<1..107)
                     /locus_tag="ACHOQQ_RS25980"
                     /old_locus_tag="pgaptmp_005196"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGU4473298.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTD"
     gene            complement(524..>933)
                     /gene="dolP"
                     /locus_tag="ACHOQQ_RS25985"
                     /old_locus_tag="pgaptmp_005197"
     CDS             complement(524..>933)
                     /gene="dolP"
                     /locus_tag="ACHOQQ_RS25985"
                     /old_locus_tag="pgaptmp_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU4473299.1"
                     /translation="ALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALG
                     VDGVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFL
                     LGLVTEQEGRAAADIASRVSGVKRVTTAFSCIR"
BASE COUNT          231 a          240 c          220 g          242 t
ORIGIN      
        1 aaatcggtca ttgtgcgccc ctggatttct gcagcgcgct tcagcatggc atgtagttct
       61 gtgctgatcc ttgcttccag tcgggcagtt tgtgttgcag atggcatgag ttgtacctcc
      121 tgaatacact gtacggcaaa ttgccgtatt gtgcaaatta cactctgaca ccgggacggc
      181 agacaggcat tctgcctgaa aagaaaaatc agattcagac aaaactatcc gggggatgtt
      241 aacgccacga tctttgacca ttgcatacag ataaaagctc agattattta ccagcagcga
      301 catccggagt acaccgggat tttatctgtg agtgaacata cccgggcaaa aaaatccccc
      361 gaattcaggg ggtgtcaaag aggaattatg tatattggtt attataatga gcacgtctcc
      421 ggtattccgc gacgaatgtt cacgtggtgg catgaacggc ccggaaacgt gctcttctga
      481 tttttaacgt aaatttacac taaaggcaac tctgatacgg ccttcaccgt atacaggaga
      541 atgccgtcgt gacccgcttc acgccactca ccctactggc aatatctgct gccgctcgcc
      601 cttcctgctc tgtgaccagg cccagtaaaa agacttcgct gttttctgtc accaccttga
      661 cactggatga tttgacacgg gcacttgtga gtaactgaga acgaattttt gtcgtgagcc
      721 aggcatcacc tgccattatg accggactga cgggagtccc ctgccggatt tcattataga
      781 cctcctgaac accatcgaca ccgagtgcta tctgacgggc acgggcagat aattcacggt
      841 ccggagactg accgatcagc agcacgcgtc cctgatatgc aatctcactg atgcgtgctt
      901 cttttctgat ttgttcatct tttgaaagtg cac
//
LOCUS       DAZKFU020000609          927 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZKFU020000609 DAZKFU020000000
VERSION     DAZKFU020000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.619"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            213..395
                     /locus_tag="ACHOQQ_RS25990"
                     /old_locus_tag="pgaptmp_005198"
     CDS             213..395
                     /locus_tag="ACHOQQ_RS25990"
                     /old_locus_tag="pgaptmp_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4473300.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPITP"
     gene            421..666
                     /locus_tag="ACHOQQ_RS25995"
                     /old_locus_tag="pgaptmp_005199"
     CDS             421..666
                     /locus_tag="ACHOQQ_RS25995"
                     /old_locus_tag="pgaptmp_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4473301.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGEPTPDSDMMM"
     gene            743..>927
                     /locus_tag="ACHOQQ_RS26000"
                     /old_locus_tag="pgaptmp_005200"
     CDS             743..>927
                     /locus_tag="ACHOQQ_RS26000"
                     /old_locus_tag="pgaptmp_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4473302.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIK"
BASE COUNT          245 a          207 c          270 g          205 t
ORIGIN      
        1 catatacgtt gctgattgac agtgtgaagg tggaagtgaa caacgcggat gccgcggcat
       61 gataaaaaag gccgccagca atgaaggaaa gggatgaact ttgtccacta catgaatgct
      121 ggcggctggt aaaacgacca tgatgacacc gaagtctgcg gtgtttttct acaaaaataa
      181 gtaaatcatg tcaactatca cggtgagaaa ccatgacatt tctgaaccag ttaatgctgt
      241 acttctgtac ggtggtctgt gtgctgtatc ttctttcggg tgggtacagg gcagtgcgcg
      301 atttctggcg caggcagatt gataaaaggg ccgcagagaa aatcagcgcc agtcagtcag
      361 ccggaagcaa acccgaagag cccattactc cttaataacc cctttcaacg agaaaatccc
      421 atgtcagaaa ttacatccct ggtcactgca gaggcagtga aggaagtcct gcgctctgaa
      481 gaagtcctga gcgcactgaa acagaaactt cgccataacc tggaagcgcg tcttgatgca
      541 gaagtggatg ccattctgga tgaactgctg ggcgcaccgg cagctccgga gccggaaggc
      601 atcgcgggtg aggggagtgc ttcagatagc ggtgaaccca cacctgacag cgacatgatg
      661 atgtaagcat gcgcaaggga ccatcggtgt gtgccggtgg tctttatatt gttgtgagct
      721 tccggattgc gggagacggg gtatgtacca gatggaaaaa atcacaacag gtgtgtcata
      781 caccacgtca gcggtgggaa cgggctactg gttcctgcag ttgctggaca gggtttcccc
      841 gtctcagtgg gcggcaatag gcgtgctggg gagtctgctg tttgggctgc tgacatatct
      901 gacgaacctg tatttcaaaa tcaaaga
//
LOCUS       DAZKFU020000610          909 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZKFU020000610 DAZKFU020000000
VERSION     DAZKFU020000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.620"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..>909
                     /locus_tag="ACHOQQ_RS26005"
                     /old_locus_tag="pgaptmp_005201"
     CDS             2..>909
                     /locus_tag="ACHOQQ_RS26005"
                     /old_locus_tag="pgaptmp_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HGU4473303.1"
                     /translation="MILFVFLLPMIIFYQPWVNALPSTPRHASPEQLEKTVRYLTQTV
                     HPRSADNIDNLNRSAEYIKEVFVSSGARVTSQDVPITGGPYKNIVADYGPADGPLIII
                     GAHYDSASSYENDQLTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPH
                     FFRSDEMGSAVHAASLEHPVKLMIALEMIGYYDSAPGSQNYPYPAMSWLYPDRGDFIA
                     VVGRMQDINAVRQVKAALLSSQDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAIMITD
                     TAFYRNKQYHLPGDTADRLNYQKMAQV"
BASE COUNT          235 a          211 c          211 g          252 t
ORIGIN      
        1 tattatatta tttgtgtttt tactccctat gattattttt taccaaccct gggtcaatgc
       61 attaccgtca acgccccgac acgcaagtcc tgagcaatta gaaaaaacag ttcgttatct
      121 tacacaaact gtgcatccac gtagtgccga caatattgat aatctgaata ggtccgcaga
      181 gtatataaaa gaggtctttg tcagtagcgg tgccagagtt acctcgcagg atgtccccat
      241 tacgggtggc ccctacaaaa acattgttgc tgattatggt cctgccgatg gaccgctgat
      301 tattattggt gcgcattatg actctgccag cagttatgaa aacgatcaat tgacctatac
      361 gccgggcgcg gatgataacg ccagcggtgt ggcaggatta ctcgaactgg cacgtttgtt
      421 acatcagcaa gtaccgaaaa caggcgtgca gctggtcgcc tatgcgtcgg aagaaccgca
      481 cttctttcgt agcgatgaaa tggggagcgc ggtgcatgca gcttcgcttg agcatccagt
      541 gaaattaatg atagcactgg agatgattgg ctattacgac tctgcgcctg gaagccagaa
      601 ttacccttac ccggcaatgt cctggcttta tcccgatcgg ggagacttta ttgccgtggt
      661 cggcagaatg caggatatca acgccgttcg tcaggtaaaa gcggcattgt tgtcatctca
      721 ggatttatct gtttattcta tgaatgcccc agggtttatt cccggtattg atttctctga
      781 tcacctgaat tattggcaac acgatattcc cgccataatg attactgaca ccgcttttta
      841 tcgtaataaa caataccact tgcccggtga taccgcagac agattgaatt atcagaaaat
      901 ggctcaggt
//
LOCUS       DAZKFU020000611          906 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZKFU020000611 DAZKFU020000000
VERSION     DAZKFU020000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.621"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>906
                     /locus_tag="ACHOQQ_RS26010"
                     /old_locus_tag="pgaptmp_005202"
     CDS             <1..>906
                     /locus_tag="ACHOQQ_RS26010"
                     /old_locus_tag="pgaptmp_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4473304.1"
                     /translation="KFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQSNGRW
                     ICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDALKDPN
                     GVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPARVVYLTAGIDSQRNRFEM
                     YVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTG
                     GIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEIL
                     YARMKADPTPADEATSYAIRFPDDPE"
BASE COUNT          214 a          218 c          277 g          197 t
ORIGIN      
        1 aaatttggcg atgagtccac gccttttggg cttaaatggg agaaggacag ccccgaaagc
       61 gttttctacc tctgtgaaca tcatggctgc gtgatccatc agtctgagct tgaccagagc
      121 aacgggcggt ggatctgtga aaacacgggg atgtggaccc gtgatggtct gacgtttttc
      181 agcgctgcgg gtaatgaaat tccgccgccg cgctccatca cgttccatat ctggacggcg
      241 tacagtccgt tcaccacctg ggtacagata gtctatgact ggctggatgc actgaaagat
      301 cccaacggcg tgaaaacctt tgtgaacacc acgctgggcg agacctggga agaggccgtg
      361 ggcgaaaaac tcgatcacca ggtactgatg gataaggttg tgcgttacac ggcggcggtg
      421 cctgcccggg tggtttatct gacggcgggc attgactcgc agcgaaaccg ttttgagatg
      481 tatgtctggg gatgggctcc gggagaggaa gcctttctgg tggataaaat catcattatg
      541 gggcgtcctg atgaggaaga gacgctgtta cgtgtggatg cggcgatcaa caaaaaatac
      601 cgccatgcag acggaaccga aatgaccatt tcccgggtct gctgggacac cggggggatc
      661 gatggcgaaa tcgtttatca gcggtcaaaa aaacacggtg ttttccgggt gctgccggta
      721 aaaggcgcat ctgtctatgg caagccggtg atcaccatgc caaaaacccg caatcagcgg
      781 ggcgtgtatc tgtgtgaagt ggggacggac actgcaaaag aaattctcta tgcccgtatg
      841 aaagccgatc ccacgcctgc ggatgaggcc acgtcgtatg ccatccgttt tcctgatgat
      901 ccggag
//
LOCUS       DAZKFU020000612          906 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZKFU020000612 DAZKFU020000000
VERSION     DAZKFU020000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.622"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..433
                     /locus_tag="ACHOQQ_RS26015"
                     /old_locus_tag="pgaptmp_005203"
     CDS             <1..433
                     /locus_tag="ACHOQQ_RS26015"
                     /old_locus_tag="pgaptmp_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HGU4473305.1"
                     /translation="YRDGDKINGPQAHSLDSTNERRFADNRTMFTQHIEKQRNVHTLN
                     QNDINNSVNSVNVPENELTYQFQRWGQNHTVRILESSEGIRLKPSDALVSDRLHEAQN
                     NDVTAQRWVLTEQDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            393..906
                     /locus_tag="ACHOQQ_RS26020"
                     /old_locus_tag="pgaptmp_005204"
                     /pseudo
     CDS             393..906
                     /locus_tag="ACHOQQ_RS26020"
                     /old_locus_tag="pgaptmp_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT          317 a          161 c          203 g          225 t
ORIGIN      
        1 ataccgcgat ggagacaaaa taaatggacc gcaggctcat tcattagaca gtactaacga
       61 aagaaggttt gctgataaca gaaccatgtt tactcaacat atcgaaaagc aaaggaatgt
      121 tcatacgctc aatcagaatg atataaataa ttccgttaat agtgtcaatg tgccagaaaa
      181 tgaattgaca tatcagtttc agcgttgggg acagaatcat acagtccgga ttctggaaag
      241 tagtgaaggg atacgactaa aaccatcaga cgcgcttgtc tccgacagac tccatgaagc
      301 ccaaaacaat gatgttacag cacaacgttg ggtattgact gagcaagatg aacgacaagg
      361 acaacgacat cagcctcatg aggagcaaga aaatgaaggc aaattcgaaa acgatcagaa
      421 ggatgaaagt taatgttcgg tctgaggaaa gtaaatcgaa acactcatac gtttgaaacc
      481 acttttcaaa attggaaaga aaatggcgaa gatgttgcat tattaatgcc cgaatttagt
      541 gctaaatggc taccaatagc agaagagagt ggcagctggt ctggatgggt attgcttcga
      601 gaaatatttc ccttaatttc ttctgagctc gctgggatgg ctttaatgcc agaaacggaa
      661 aggttaatag gggaatggct cagtttatcc agttccccgt taaatctaaa gcatcctgaa
      721 ctaaaatata atcgtttgtg cgtaggtaaa gtatttgatg gagtattgag cccagctcaa
      781 ccactaataa gaatatggac aggggaattg aatctttggt tagataaagt cacagtctgc
      841 caatacggaa acgctccagc gttagacaaa aaatcattat attggcccat tcattttgta
      901 attgga
//
LOCUS       DAZKFU020000613          905 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZKFU020000613 DAZKFU020000000
VERSION     DAZKFU020000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.623"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..305)
                     /locus_tag="ACHOQQ_RS26025"
                     /old_locus_tag="pgaptmp_005205"
     CDS             complement(<1..305)
                     /locus_tag="ACHOQQ_RS26025"
                     /old_locus_tag="pgaptmp_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473306.1"
                     /translation="MKVVFNVKSDFDKIFLPLEFSGFHGRNGYCYLRVQIKRGFIVFS
                     CAQLLNYYRTSVTNAIEQVREAAVNVLFREGVLSYTQQKEFWDLLKTSQRVNKEIDS"
     gene            complement(483..>905)
                     /locus_tag="ACHOQQ_RS26030"
                     /old_locus_tag="pgaptmp_005206"
     CDS             complement(483..>905)
                     /locus_tag="ACHOQQ_RS26030"
                     /old_locus_tag="pgaptmp_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4473307.1"
                     /translation="SVFMQLELGDKYRSQFFTPWSVACMMAQMQLGDVKALFESNPFI
                     TLSEPACGAGSMILAMADTLNRSGYPAYQRMWVSATDIDPLAAGMAYIQLSLCGVAGE
                     VVIGNSLCNERRRVLLTPGHYLGNWDHRLRHLQEQNVA"
BASE COUNT          270 a          238 c          172 g          225 t
ORIGIN      
        1 tgcgaatcaa tttctttgtt tacccgttgt gatgtcttta gtaaatccca gaattctttt
       61 tgctgtgtgt atgacaacac accttccctg aagaggacat taaccgcagc ctctctgacc
      121 tgctcaattg catttgttac tgacgtacga tagtagttca ataattgagc gcatgagaaa
      181 acaataaaac cacgcttaat ctgaactctt aaataacagt acccattccg cccatgaaaa
      241 ccagaaaact ccagtggtaa aaaaatctta tcgaaatcac tttttacatt aaagacaacc
      301 ttcacttaac taaactcctg tttgatatct tgagaatata attaatcctg agtttataaa
      361 atgaatagcg atgaccaagc tgtcaacatg gcatacataa acttaaaaaa aacaacaaaa
      421 actgacgttg cagatgtgat aacgccagtc cgaagactgg cgctgtgata aaacattctg
      481 catcatgcca cattctgctc ctgaagatga cgcagacgat ggtcccagtt tcccaggtaa
      541 tgcccgggag tcagcagtac gcggcgtcgc tcattacaga gcgagttacc aatcaccact
      601 tctccggcca caccacacag cgacagttgt atatacgcca tgccggctgc cagcgggtca
      661 atatccgttg ccgacaccca catcctctgg tatgccggat accccgaacg gttcagggtg
      721 tcagccattg ccagaatcat actgccggca ccacaggcag gttcactgag cgtgataaag
      781 gggttactct caaacaacgc cttcacatct cccagttgca tctgtgccat catgcaggca
      841 acactccagg gcgtgaaaaa ctgactccgg tatttatccc ccagttccag ttgcatgaac
      901 acact
//
LOCUS       DAZKFU020000614          905 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZKFU020000614 DAZKFU020000000
VERSION     DAZKFU020000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.624"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            168..347
                     /locus_tag="ACHOQQ_RS26035"
                     /old_locus_tag="pgaptmp_005207"
     CDS             168..347
                     /locus_tag="ACHOQQ_RS26035"
                     /old_locus_tag="pgaptmp_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4473308.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQNGGNKSGKS"
     gene            433..>905
                     /locus_tag="ACHOQQ_RS26040"
                     /old_locus_tag="pgaptmp_005208"
     CDS             433..>905
                     /locus_tag="ACHOQQ_RS26040"
                     /old_locus_tag="pgaptmp_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4473309.1"
                     /translation="MNMKTMEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNV"
BASE COUNT          307 a          186 c          203 g          209 t
ORIGIN      
        1 ttcctcaggc aattgttaat atatcaagaa cgttcctcat aatatatttt cccacttcct
       61 tctcgttacg cttaatttga ccaattctca ttatcgacta attttaatga gtgtcgacac
      121 acaacactca tattaatgaa acaatgcaac gcaacgggag aaataacatg gccgaacatc
      181 gtggtggttc aggaaatttc gccgaagacc gtgagaaggc atccgacgca ggccgtaaag
      241 gcggtcagca tagcggcggt aattttaaaa atgatccgca acgcgcatct gaagcgggta
      301 aaaaaggcgg tcaacaaaac ggtggtaata aatcaggtaa atcctgattc accgttaatt
      361 attaatatca gctgataaag ataattgctt ctgcgggccg tatggctcgc agtgattcca
      421 ctggagaaaa atatgaatat gaagaccatg gaagatgtat ttattcacct actttcagat
      481 acctacagcg cagaaaaaca attaacccgg gcactggcaa aactcgcaag agcaacatca
      541 aacgaaaaat taagtcaggc ttttcatgcg cacctcgagg aaacacatgg acagattgaa
      601 cgtattgatc aagttgtgga atcagaatcg aatctgaaaa ttaagcgcat gaaatgtgtg
      661 gcaatggaag gtcttattga agaagctaat gaggtcatcg aaagtaccga gaaaaacgaa
      721 gtgcgtgatg ccgcactgat tgccgcagca cagaaagtcg agcattatga gattgccagt
      781 tacgggacat tagcgacgct ggctgaacaa ttaggttacc gtaaagcagc gaagcttctg
      841 aaagagaccc tggaagaaga aaaggccacc gacatcaaac tgactgatct ggcccttaat
      901 aacgt
//
LOCUS       DAZKFU020000615          904 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZKFU020000615 DAZKFU020000000
VERSION     DAZKFU020000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.625"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>904
                     /locus_tag="ACHOQQ_RS26045"
                     /old_locus_tag="pgaptmp_005209"
     CDS             <1..>904
                     /locus_tag="ACHOQQ_RS26045"
                     /old_locus_tag="pgaptmp_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473310.1"
                     /translation="LHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYD
                     AAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHEKAVMNYMEYLVTSQLDEQQMS
                     SLLYERDDLSAIDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSKRRILININHDF
                     PNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNDDGSTRVG
                     GEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPH
                     DGLVIINKGNEEVEGTWPNKLQPGK"
BASE COUNT          317 a          175 c          171 g          241 t
ORIGIN      
        1 cctccatgca ttaaaagaaa agcgtttaaa tggaatgcct aaaacaaccc ccaaaagtga
       61 ccaggttatt atgctgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg
      121 tttacgttat gatgcggcaa aacactcaaa acatgaacaa atagaaagat caataacccc
      181 accgcttaaa aattataatg agcgattaca caatactaac ttatttaacc caaaatatca
      241 tgaaaaagcc gttatgaatt acatggaata tctggtaact agtcagttgg atgaacaaca
      301 aatgtcatcg ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat
      361 gaagacgata aaagcttttt catttggtgg cgatctccaa acccttgcat caaaaccggg
      421 ttcaacaatc tcaagcatcc catcaaaaag acggatattg attaacatta accacgattt
      481 tcctaacaat ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt
      541 agcaatggca tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca
      601 agtattaaaa tcaacgactg aaattaaaaa tgatgatggg tcgacgcgtg tcggcggtga
      661 ggcgtggctt aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt
      721 atatatagat aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt
      781 tctggccttt agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt
      841 tataataaat aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg
      901 aaaa
//
LOCUS       DAZKFU020000616          903 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZKFU020000616 DAZKFU020000000
VERSION     DAZKFU020000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.626"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..886
                     /gene="hlyE"
                     /locus_tag="ACHOQQ_RS26050"
                     /old_locus_tag="pgaptmp_005210"
     CDS             <1..886
                     /gene="hlyE"
                     /locus_tag="ACHOQQ_RS26050"
                     /old_locus_tag="pgaptmp_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HGU4473311.1"
                     /translation="VEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQE
                     YSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASA
                     QKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQ
                     VDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFFTTLSN
                     TVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTC
                     NEYQKRHGKKTLFEVPEV"
BASE COUNT          298 a          177 c          200 g          228 t
ORIGIN      
        1 ggtagaggta gttaaaaacg caatcgaaac cgcagatgga gcattggatc tttataataa
       61 atatctcgat caggtcatcc cctggcagac ctttgatgaa accataaaag agttaagtcg
      121 ctttaaacag gagtattcac aggcagcctc cgttttagtt ggcgatatta aaaccttact
      181 tatggatagc caggataagt attttgaagc aacccaaaca gtgtatgaat ggtgtggtgt
      241 tgcgacgcaa ttgctcgcag cgtatatttt gctatttgat gagtacaatg agaagaaagc
      301 atccgcccag aaagacattc tcattaaggt actggatgac ggcatcacga agctgaatga
      361 agcgcaaaaa tccctgctgg taagctcaca aagtttcaac aacgcttccg ggaaactgct
      421 ggcgttagat agccagttaa ccaatgattt ttcagaaaaa agcagctatt tccagtcaca
      481 ggtagataaa atcaggaagg aagcgtatgc cggtgccgca gccggtgtcg tcgccggtcc
      541 atttggatta atcatttcct attctattgc cgcgggcgta gttgaaggga aactgattcc
      601 agaattgaag aacaagttaa aatctgtgca gagtttcttt accaccctgt ctaacacggt
      661 taaacaagcg aataaagata tcgatgccgc caaattgaaa ttaaccaccg aaatagccgc
      721 catcggggag ataaaaacgg aaactgaaac aaccagattc tatgttgatt atgatgattt
      781 aatgctttct ttgctaaaag aagcggcaaa aaaaatgatt aacacctgta atgagtatca
      841 gaaaagacac ggtaaaaaga cactctttga ggtacctgaa gtctgatcag cgattattct
      901 ctc
//
LOCUS       DAZKFU020000617          902 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZKFU020000617 DAZKFU020000000
VERSION     DAZKFU020000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.627"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..290)
                     /locus_tag="ACHOQQ_RS26055"
                     /old_locus_tag="pgaptmp_005211"
     CDS             complement(<1..290)
                     /locus_tag="ACHOQQ_RS26055"
                     /old_locus_tag="pgaptmp_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4473312.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTLLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSPVYFRDAVAFSSLALFEEHPDF"
     gene            complement(482..901)
                     /locus_tag="ACHOQQ_RS26060"
                     /old_locus_tag="pgaptmp_005212"
     CDS             complement(482..901)
                     /locus_tag="ACHOQQ_RS26060"
                     /old_locus_tag="pgaptmp_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473313.1"
                     /translation="MSAPFLVAGCDDATKENVSKKAADIKEAAQNQLSDVKDAAEDVK
                     MQAAGKVAEVSEAAQSGLSEVKDAVEEVKTQAAGKMAEVSEAAQSGLSEVKDAVEEVK
                     TQAAGKVAEVSEATQSGLSDVKDTAPEKGAEISVTEE"
BASE COUNT          209 a          273 c          205 g          215 t
ORIGIN      
        1 tggaaatccg gatgttcctc aaacagcgcc agactggaaa aagccaccgc atccctgaag
       61 tacaccggcg atggttcatc tgtaataagt tgtctgatca gacattccgc cagcacggac
      121 atctgtggcc ggatctgtaa aagcaaagac gacgggctga ctgccatacc ggataatagc
      181 gtgcagaacc gcagataccg cctcaccacc tcctcactga ggtcagcgat aaggaaacgt
      241 ccggtatgag cctgaaaagc agggcgcaga tgcccggcaa tcagaagcat tgtgccggca
      301 ggtatgacag agttcctgtg ctccagacaa agcattgtct gtttgagagc cataatgaca
      361 gcgcaggtac agggcatcgg atatccggta aatatgctga tttattaaag agacagacat
      421 gccccgtata taaaattccg tgtcaccggg tgctgaaaaa aactgcaccg gcggcgctct
      481 tttactcctc cgttacggat atttcagccc ccttttcagg agcggtatcc ttcacatcac
      541 tgagaccgga ctgagtcgct tcagagacct ctgccacctt tccggctgcc tgtgttttta
      601 cttcctccac ggcatccttc acctcgctga gcccggactg agccgcttca gagacctctg
      661 ccatctttcc ggctgcctgt gttttcactt cctccacggc atccttcacc tcgctgagcc
      721 cggactgagc cgcttcagaa acctctgcca cttttccggc agcctgcatt ttcacgtcct
      781 ccgcggcatc cttcacatca ctgagttgat tctgcgcagc ttcctttata tctgccgcct
      841 tcttacttac attctccttt gtcgcatcat cacagcccgc aaccagaaat ggagcggaaa
      901 ta
//
LOCUS       DAZKFU020000618          896 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZKFU020000618 DAZKFU020000000
VERSION     DAZKFU020000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.628"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            187..789
                     /gene="fimB"
                     /locus_tag="ACHOQQ_RS26065"
                     /old_locus_tag="pgaptmp_005213"
     CDS             187..789
                     /gene="fimB"
                     /locus_tag="ACHOQQ_RS26065"
                     /old_locus_tag="pgaptmp_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGU4473314.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          265 a          164 c          192 g          275 t
ORIGIN      
        1 cgtttgtatg gcaacgttat tataattaac tgttgctaat ccatttaagt tcactcagaa
       61 gaactggtcc acttacgtta gttattaagc aaacgttcgc ttttataaac ataatcagga
      121 taaaaatgtt ggattattgc taacccagca cagctagtgc gcgtctgtaa ttataaggga
      181 aaaacgatga agaataaggc tgataacaaa aaaaggaact tcctgaccca tagtgaaatc
      241 gaatcactcc ttaaagcagc aaataccggg cctcatgcag cacgtaatta ttgtctgact
      301 ttgctttgtt ttattcatgg tttccgggcg agtgaaattt gtcgattgag gatttcggat
      361 attgatctta aggcaaagtg tatatatatc catcgattaa aaaaaggctt ttcaacaacg
      421 cacccgctat tgaacaaaga agttcaggct ttaaaaaact ggttgagtat ccgtacttcg
      481 tacccgcatg ctgagagcga gtgggtattt ttatcgcgta aggggaatcc gctttctcgg
      541 caacagtttt accatattat ctcgacttcc ggtggtaatg ccgggttgtc actggagatt
      601 catccgcaca tgttacgcca ttcgtgtggt tttgctttgg cgaatatggg aatagatacg
      661 cgacttatcc aggattatct tgggcatcgc aatattcgtc atactgtctg gtataccgcc
      721 agcaatgcag ggcgttttta cggcatctgg gatagagcca gaggacgaca gcgtcacgct
      781 gttttataga gatagttgag ataccaggga tggtgttaaa aactacatta ttcttacgat
      841 atatcaaaaa tgattaacca tttattaatt gatgttattg taagtataat tcttaa
//
LOCUS       DAZKFU020000619          887 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZKFU020000619 DAZKFU020000000
VERSION     DAZKFU020000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.629"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(294..>887)
                     /locus_tag="ACHOQQ_RS26070"
                     /old_locus_tag="pgaptmp_005214"
     CDS             complement(294..>887)
                     /locus_tag="ACHOQQ_RS26070"
                     /old_locus_tag="pgaptmp_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4473315.1"
                     /translation="AHTGRFLIADLSEEVVRRYLRFCTPLSGMAVSPSSLLLQIRPQM
                     SVLAECLIRQLITDEPSSVYFRDAVAFSSLALFEEHPDFHALLTGCIRSCTVRVKNII
                     SADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQYAAQLLSAGENRI
                     SRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
BASE COUNT          267 a          235 c          204 g          181 t
ORIGIN      
        1 aaaaatgtat cctgcgtctg ctcaggagca tcaagcagga caacacgaac acctgctggc
       61 gggttcatgg ggcgctggtg cgaatcggta taaattgttg taccggcaga tgcagccacc
      121 ggtagtatca ggaaggaata cagtaacagt cggtacatga tgcgactccg tcagacaact
      181 ggtatctgac agcgtgcgca aaggcaaagg gacaatcagc aataaattca aaaccggatg
      241 ataaaaatta agcctcttcc tctgaataac gcacactctg catctgaatc aaattaatcc
      301 ggttgcatca caggaagcca ctgattcggg gtaataccat aataacgcct gaacacagaa
      361 ataaaataag aagcactggc atagccacac aactctgcca cccgggaaat acggttttct
      421 cccgcagaca aaagctgagc ggcatactgc attctctctt tcagaagaag ctcactgaag
      481 gtcgtcagct cttcctgaag ctttttcttc agtaaactct cacttatata taacagactg
      541 gccacttcac ccagcatcca tggacgggac aaatcagcac tgataatatt cttcacccgg
      601 acagtgcagc tccggataca ccccgtgagc agggcgtgga aatccggatg ttcctcaaac
      661 agcgccagac tggaaaaagc caccgcatcc ctgaagtaca ccgacgatgg ttcatctgta
      721 ataagttgtc tgatcagaca ttccgccagc acggacatct gtggccggat ctgtaaaagc
      781 aaagacgacg ggctgactgc cataccggat aatggcgtgc agaaccgcag ataccgcctc
      841 accacctcct cactgaggtc agcgataagg aaacgtccgg tatgagc
//
LOCUS       DAZKFU020000620          876 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZKFU020000620 DAZKFU020000000
VERSION     DAZKFU020000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.630"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            159..845
                     /locus_tag="ACHOQQ_RS26075"
                     /old_locus_tag="pgaptmp_005215"
     CDS             159..845
                     /locus_tag="ACHOQQ_RS26075"
                     /old_locus_tag="pgaptmp_005215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4473316.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT          237 a          191 c          224 g          224 t
ORIGIN      
        1 attcaaccgt tagtacagtc aggaaatagt ttagcctttt ttaagctaag taaagggctt
       61 tttctgcgac ttacgttaag gatttgtaaa ttcgcaccgc gtaataagtt ggcagtgatc
      121 accctgttcg cggtcatttg gtcaagaaga gtggcaatat gcgtataacg attattctgg
      181 tcgcacccgc cagagcagaa aatattgggg cagcggcgcg ggcaatgaaa acgatggggt
      241 ttagcgaact gcggattgtc gatagtcagg cacatctgga gccagccact cgctgggtcg
      301 cacatggatc tggtgatatt attgataata ttaaagtttt cccgacgctg gctgaatcgt
      361 tacacgatgt cgatttcact gtcgccacca ctgcacgcag tcgggcgaaa tatcattact
      421 acgccacgcc agtagaactg gtgccgctgt tagaggaaaa atcttcatgg atgagccatg
      481 ccgcgctggt gtttggtcgc gaagattccg ggctgactaa cgaagagtta gcgttggctg
      541 acgttcttac tggtgtgccg atggtggcgg attatccttc gctcaatctt gggcaggcgg
      601 tgatggtcta ttgctatcaa ttagcaacat taatacaaca accgacgaaa agtgacacaa
      661 cggcagacca acatcaactg caagctttac gcgaacgagc catggcattg ctgacgactc
      721 tggcagtggc agatgacata aaactggtcg actggttaca acaacgcctg gggcttttag
      781 agcaacgaga cacggcaatg ttgcaccgtt tgctgcatga tattgaaaaa aatatcacca
      841 aataaaaaac gccttagtaa gtatttttca gctttt
//
LOCUS       DAZKFU020000621          876 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZKFU020000621 DAZKFU020000000
VERSION     DAZKFU020000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.631"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..457
                     /gene="yhaJ"
                     /locus_tag="ACHOQQ_RS26080"
                     /old_locus_tag="pgaptmp_005216"
     CDS             <1..457
                     /gene="yhaJ"
                     /locus_tag="ACHOQQ_RS26080"
                     /old_locus_tag="pgaptmp_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU4473317.1"
                     /translation="IVIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSE
                     VTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMV
                     EKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFNGK"
     gene            complement(508..864)
                     /gene="yhaI"
                     /locus_tag="ACHOQQ_RS26085"
                     /old_locus_tag="pgaptmp_005217"
     CDS             complement(508..864)
                     /gene="yhaI"
                     /locus_tag="ACHOQQ_RS26085"
                     /old_locus_tag="pgaptmp_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4473318.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
BASE COUNT          262 a          195 c          226 g          193 t
ORIGIN      
        1 tattgttatc gcgccggata tgcattttcg ttcctcgtcg gagatcaact cgcgcaagct
       61 ctatacgtta atgaacgtct acgttgccgc gcctgatcac ccgattcatc aggagccgga
      121 accgttatct gaagtgacgc gtgtgaaata tcgtggaatt gcggtggcgg ataccgctcg
      181 tgagcgcccg gtgttgaccg tacagctgct ggacaaacag ccgcgcttaa cggtgagcac
      241 gattgaagat aaacgtcagg cattactggc ggggcttggc gtggcgacga tgccgtatcc
      301 gatggtcgaa aaagatattg cggaagggcg gttgcgtgtc gtcagcccgg aatcgaccag
      361 cgagatcgat attattatgg cctggcgtcg tgacagtatg ggggaagcga aatcctggtg
      421 tctgcgggaa attcccaaac tttttaacgg aaaataatat ttattattgt taataaaaag
      481 gggcgaaata tcgccccaat tctaagatta atttgaacca aacttcggat cgtttccgta
      541 acgattgctg ccagaagtac cttctgtgca gaaaaaaacc agaagcacga gccagccaat
      601 aaacggaaca aaaaagagca aggcccaggc acctgaacgg tcggtatcgt gtaagcgacg
      661 gatagcaagc gcaaggacgg gaaggaaggt tgccagcaaa taaagcatag acagatatgg
      721 aagctccaga cctaaaatca attgaatgac attgataata gcgccgacga tggcgttaat
      781 cagagtaaac atccagtact ctttacgacg tgcgcgacca gagaaaccaa cataattttt
      841 taatacggat aaataccact gcataaaaac atccct
//
LOCUS       DAZKFU020000622          875 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZKFU020000622 DAZKFU020000000
VERSION     DAZKFU020000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.632"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>875
                     /locus_tag="ACHOQQ_RS26090"
                     /old_locus_tag="pgaptmp_005218"
     CDS             <1..>875
                     /locus_tag="ACHOQQ_RS26090"
                     /old_locus_tag="pgaptmp_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473319.1"
                     /translation="SLFPDTTYRELNKAAQRSITAPDRIGEGKTWIYQRDTMNEREDN
                     KGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDYELAADVYNINVTSPSGQQETFK
                     ILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQQIRENMSEFARC
                     HYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAK
                     KFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIEGGIPGYWQGQVLIPFII
                     DPVTKERKQIHPEDI"
BASE COUNT          298 a          185 c          165 g          227 t
ORIGIN      
        1 ttcccttttt ccagatacaa cttaccgtga actgaataaa gctgcacagc gtagtattac
       61 agcgccagac aggataggag aaggaaaaac atggatttat caaagagata caatgaatga
      121 aagagaagat aacaaggggg ttcaccagta tggtcctgct gaacatttca cccacattat
      181 atctgacaaa ccctccccaa aagataaata tgttgcatat gctattaaca ttcctgacta
      241 tgagctggca gccgatgtat ataatattaa cgtgacgtca ccttccggac agcaagaaac
      301 atttaaaata ctaatcaatc cagaacatct acggcaaaca cttgagcgta aatctcttac
      361 tgctgttcag aaatcacaat gtgaaatcat cacccccaaa aaacctggcg aagcgattct
      421 tcatgctttt aatgccacct accagcaaat cagagaaaat atgtctgaat ttgcacgttg
      481 ccattatggg tatatacaaa tccctccagt gacaactttc cgtgccgacg gaccagaaac
      541 tcccgaagaa gaaaaaggtt actggtttca cgcttatcaa cccgaagatc tttgtaccat
      601 ccacaatcca atgggagatt tgcaggattt tatcgcattg gttaaagatg ctaaaaaatt
      661 tggtatcgat atcattcctg attatacctt taactttatg ggaatcgggg ggagtggtaa
      721 aaatgacctg gattatccct ctgctgatat acgagcgaag atcagtaaag atatagaagg
      781 tggtatccct ggctattggc aaggtcaggt tttgattcca tttattatcg atccagtaac
      841 aaaagaacgt aaacaaatcc atccagaaga tatac
//
LOCUS       DAZKFU020000623          869 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZKFU020000623 DAZKFU020000000
VERSION     DAZKFU020000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.633"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..577
                     /locus_tag="ACHOQQ_RS26095"
                     /old_locus_tag="pgaptmp_005219"
     CDS             <1..577
                     /locus_tag="ACHOQQ_RS26095"
                     /old_locus_tag="pgaptmp_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HGU4473320.1"
                     /translation="PPVPERSDIIIKTDDPVPCDGIWEPVKIHYDHKLLIIKDGISGF
                     NNLGAYNYFIRGMNAPRQVYYDVLIESDTEMVVPDDPIRYRDVHWRLVWEDTRYSDGI
                     IPDESEYFLDDAPGKRITCPSGELCPHSGHWATIAGGHQQFIDVQAGQLMPEATKYQS
                     NMYADEIHLPAIWNLLRRDDEGSVYVKTGES"
     gene            637..>869
                     /locus_tag="ACHOQQ_RS26100"
                     /old_locus_tag="pgaptmp_005220"
     CDS             637..>869
                     /locus_tag="ACHOQQ_RS26100"
                     /old_locus_tag="pgaptmp_005220"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HGU4473321.1"
                     /translation="MTLFALVDGQRYFGESLSVQQPAAVPLFDTWPDSRIAFAGPWVM
                     EMNSITDFRERLCELEAALPSVSWMISSSSLTEL"
BASE COUNT          224 a          191 c          217 g          237 t
ORIGIN      
        1 cccccctgtt ccagaacgca gcgatattat tattaaaacc gatgacccgg ttccctgtga
       61 cggcatctgg gaaccggtaa agattcatta cgaccataaa ctattgatta ttaaagatgg
      121 tatcagcgga tttaataacc tcggtgctta taattatttt attcgtggaa tgaatgctcc
      181 tcgccaggtg tattacgacg tattaattga aagtgacaca gaaatggtcg tacccgatga
      241 cccaatccgt taccgggacg ttcactggcg tctggtctgg gaagacacgc gctacagtga
      301 cggaattatt cccgatgagt cagaatactt tctggatgat gcaccaggca aacgcattac
      361 ctgcccgagt ggggagcttt gcccgcattc aggtcattgg gcgaccatag ccggaggaca
      421 tcagcagttt attgatgttc aggccgggca gttgatgcca gaagcaacta aatatcagag
      481 caatatgtat gcagatgaaa tccatcttcc tgcgatatgg aaccttttac gccgggatga
      541 tgaggggagt gtttatgtaa aaacagggga aagttaaata actgatcaac gctttttgac
      601 ggacagatct gttaccacct gatttgctca acaaaagtga cattatttgc tctggtggat
      661 ggtcagcggt atttcggtga gagcctgtct gttcaacaac ctgccgccgt gcccctgttc
      721 gatacctggc ctgattcgcg tattgcattt gcggggccat gggttatgga gatgaacagt
      781 ataacggatt tcagagaaag actgtgtgag cttgaagctg cgcttccgtc agtttcctgg
      841 atgatatctt cttcctcgct tactgaact
//
LOCUS       DAZKFU020000624          867 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZKFU020000624 DAZKFU020000000
VERSION     DAZKFU020000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.634"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..311
                     /locus_tag="ACHOQQ_RS26105"
                     /old_locus_tag="pgaptmp_005221"
     CDS             <1..311
                     /locus_tag="ACHOQQ_RS26105"
                     /old_locus_tag="pgaptmp_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HGU4473322.1"
                     /translation="PIRFYQGSKVLVSENNLTATTKEVAAATNIDRAMLLGAQALANA
                     YGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQDHGVIAVDTAVKL
                     "
     gene            367..756
                     /locus_tag="ACHOQQ_RS26110"
                     /old_locus_tag="pgaptmp_005222"
     CDS             367..756
                     /locus_tag="ACHOQQ_RS26110"
                     /old_locus_tag="pgaptmp_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473323.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            806..>867
                     /locus_tag="ACHOQQ_RS26115"
                     /old_locus_tag="pgaptmp_005223"
     CDS             806..>867
                     /locus_tag="ACHOQQ_RS26115"
                     /old_locus_tag="pgaptmp_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473324.1"
                     /translation="MSEKIAVVYIGPKPVKKDTIT"
BASE COUNT          213 a          210 c          234 g          210 t
ORIGIN      
        1 tgccgatccg tttctatcag gggtcaaagg ttctggtatc agagaataac ctgacggcaa
       61 ccacgaaaga ggtcgctgct gcaaccaata ttgaccgcgc catgttactg ggggctcagg
      121 cgctggcaaa tgcttacggt cagaaggcgg gcggtcactt caacatggtt gagaagaaaa
      181 cggatatgga taaccgtact gagatagcaa tcagctggat caacggtctg aaaaaaatcc
      241 gtttccccga gaagagcggc aagatgcagg atcacggcgt gattgccgtt gatacagcag
      301 tgaagctctg attttttcct ttccccatgc cgggttttcg cccggctttt tcaggagtca
      361 ttaattatgg caaagactat ccttgccccg tcactgagtg aacgggtcta tacgggtacg
      421 cacggtaatg agtcggtggc agaaggcgta tttacggtga atgctgcgga agcggacagt
      481 gttattcatc ttctctcact gccagtgggc atccgtatca actcactcca gctggtttca
      541 acgggtggtc tgggtactgc aaccgtcagc attaagtccg gtgagcatgc tctcatcgat
      601 aacagcgaag ctgtttctgc aaaatttgcc agatatgtgc cagtggagcc gtacaccaca
      661 cagcgtgacg gggagctggt tactgtcacc attaagactg ccgctgcaac cggcaccctg
      721 aatgttctgc tgcgttatac cgtggtggga tactgattaa aaccttccgg cccgcgtcat
      781 gcgggctttt tatccgggga attatatgag tgagaaaatt gccgttgtct atatcggccc
      841 aaaacccgtg aaaaaggaca ccattac
//
LOCUS       DAZKFU020000625          863 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZKFU020000625 DAZKFU020000000
VERSION     DAZKFU020000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.635"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..820
                     /locus_tag="ACHOQQ_RS26120"
                     /old_locus_tag="pgaptmp_005224"
     CDS             <1..820
                     /locus_tag="ACHOQQ_RS26120"
                     /old_locus_tag="pgaptmp_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HGU4473325.1"
                     /translation="ARKIHLYHCDHRGLPLALISTEGATEWCAEYDEWGNLLNEENPH
                     LLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISD
                     IDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKE
                     FAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEI
                     FSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNGKGLPNVCRVHGNM"
BASE COUNT          270 a          165 c          211 g          217 t
ORIGIN      
        1 ggcgcgaaaa atccacctgt accactgcga ccatcgcggc ctgccgctgg cgcttatcag
       61 cacggaaggg gctacagaat ggtgcgcaga atacgatgag tggggcaacc tgctgaatga
      121 agagaacccg catctgctgc agcagcttat ccgcctgccg gggcagcagt atgatgagga
      181 gtccggcctg tattacaacc gccaccgcta ttatgacccg ctgcagggga ggtatatcac
      241 tcaggatccg attgggctga aggggggatg gaacctgtat ggatatcaat tgaatccgat
      301 atcagacatc gaccccctgg gtttatctat gtgggaggat gcaaaatcgg gggcatgtac
      361 taatggtctt tgcggcacac tatccgctat gataggtcca gataaatttg attctataga
      421 tagcaccgca tatgacgcct taaataaaat aaatagccaa tctatttgcg aagataaaga
      481 gttcgctggt ttaatatgta aggataatag tggcagatat ttctcaacag cacctaaccg
      541 aggagaaaga aaaggatcat atccattcaa tagcccttgc cctaatggta ctgagaaagt
      601 atcagcttat catactcatg gtgcagatag tcatggagaa tattgggacg aaatattttc
      661 aggtaaagat gagaaaatag ttaaaagtaa agataacaat atcaagtcat tttatttagg
      721 tacgcccagt ggtaatttta aagcaataga taaccacggg aaggaaataa caaacggaaa
      781 aggattacct aatgtctgca gagttcatgg taatatgtaa aaaatattgt ttaggaactg
      841 tgtcattgta tctttgtttg ttt
//
LOCUS       DAZKFU020000626          854 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZKFU020000626 DAZKFU020000000
VERSION     DAZKFU020000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.636"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..113
                     /locus_tag="ACHOQQ_RS26125"
                     /old_locus_tag="pgaptmp_005225"
     CDS             <1..113
                     /locus_tag="ACHOQQ_RS26125"
                     /old_locus_tag="pgaptmp_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4473326.1"
                     /translation="QHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK"
     gene            203..577
                     /gene="cbtA"
                     /locus_tag="ACHOQQ_RS26130"
                     /old_locus_tag="pgaptmp_005226"
     CDS             203..577
                     /gene="cbtA"
                     /locus_tag="ACHOQQ_RS26130"
                     /old_locus_tag="pgaptmp_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4473327.1"
                     /translation="MKTLPVLPGQSASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            574..>854
                     /locus_tag="ACHOQQ_RS26135"
                     /old_locus_tag="pgaptmp_005227"
     CDS             574..>854
                     /locus_tag="ACHOQQ_RS26135"
                     /old_locus_tag="pgaptmp_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4473328.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVG"
BASE COUNT          219 a          231 c          205 g          199 t
ORIGIN      
        1 atcagcatac cgtcacgctg tatgccagag ggctgacctg cgaagccgac accctcggca
       61 gttgtggcta cgtttatatg gctgtttatc cgacgcccga aacgaaaaag taactctcca
      121 gaataaccct ctgctccggc ctggtgtttt caccacacca ctttcccatt tttcatcttt
      181 gcatatcagg aaaatcttca gtatgaaaac attacctgta ttacccgggc agtcggccag
      241 ttcacgccca tctcctgttg aaatctggca gacactgctc acccgactgc tggaccagca
      301 ctatggcctt acgctgaatg acacaccgtt cgctgatgaa cgtgtgattg agcagcatat
      361 tgaggcaggc atttcactgt gtgatgcggt gaattttctc gtggaaaaat acgcgctggt
      421 gcgtaccgac cagccgggat tcagcgcagg aacttcgtcg cagttaatca acagcattga
      481 tattctccgg gcacgccggg caacaggtct gatgacccgc gacaattaca gaacggtaaa
      541 taacattacc ctgggtaagc atccggagaa acgatgaaac tttccctgat gctggaagcc
      601 gacagaatta atgtgcaggc actgaacatg gggcaaattg tcgttgacgt cgatggtgtt
      661 aatctcactg aactgattaa caaggtcgct gaaaacggtt attcactccg cgtggtggag
      721 gaatccgacc aacagtcaac ctgcacacta ccaccgtttg caacccttgc cggcatacgc
      781 tgcagtaccg cacatatcac ggaaaaggat aacgcctggc tgtactcgct gtcgcatcag
      841 accagtgacg ttgg
//
LOCUS       DAZKFU020000627          852 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZKFU020000627 DAZKFU020000000
VERSION     DAZKFU020000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.637"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..809
                     /locus_tag="ACHOQQ_RS26140"
                     /old_locus_tag="pgaptmp_005228"
     CDS             9..809
                     /locus_tag="ACHOQQ_RS26140"
                     /old_locus_tag="pgaptmp_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473329.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKAYVSMVKDLVSRFQ"
BASE COUNT          268 a          169 c          194 g          221 t
ORIGIN      
        1 gtccaattat gaattcaaaa aagctttgtt gcatatgtgt gttattctcg ctgcttgcag
       61 gatgtgcctc tgaatcttct attgatgaaa agaagaaaaa agctcaagtc acacaaagta
      121 atattaataa aaacactccc cagcaactga cagacaaaga tttattcggt aatgaaacca
      181 ctctggccgt atccgaagag gatattcaag cagcgcttga tggagatgag ttccgcgttc
      241 cccttaattc tcctgtaata cttgttcaat ccggcaaccg cgcaccggaa accattatgc
      301 aggaggagat gcgaaaatat tatactgttt ccacattctc tggtatcccg gacaggcaaa
      361 agcctctgac ttgtaacaaa aacaaagata aaaatgagaa cgaggatgtt gcccgtgctg
      421 agaatatgaa ctggatgcag gcactgcgtt ttgtggctgc aaaaggacat cagaaagcga
      481 ttattgttta ccaggatatg ttgcagacag gaaaatatga ctctgcgctg aaatcaacag
      541 tatggtccga ctataaaaat gaaaaactca ctgacgctat ctctctgcgc tacctggtac
      601 gtttcacgct ggtggatgtg gcaacaggtg agtgggctac ctggtcgccg gtgaattatg
      661 aatcgagagt aatttttccg ccggctggaa ttacaaaaac gagcaataaa gaactatcaa
      721 atgaccatgt tacggaagcg caactgttta atttgaaaca aaaagcatat gtatcgatgg
      781 tgaaagactt ggtgagtcga ttccagtaat cgttattgtt atgctgccgc ctgtagttta
      841 gggtggcagc aa
//
LOCUS       DAZKFU020000628          849 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZKFU020000628 DAZKFU020000000
VERSION     DAZKFU020000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.638"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..689
                     /locus_tag="ACHOQQ_RS26145"
                     /old_locus_tag="pgaptmp_005229"
     CDS             <1..689
                     /locus_tag="ACHOQQ_RS26145"
                     /old_locus_tag="pgaptmp_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HGU4473330.1"
                     /translation="MINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTF
                     SLTPEGRLTARNADISGAVTANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAF
                     PRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYSSCRLMVKC
                     NGRVLFERTALAESQVYSGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASISDLLVVVM
                     KKSTAGISIS"
BASE COUNT          202 a          203 c          261 g          183 t
ORIGIN      
        1 cgatgattaa cccggagaac ggcaacacca cgccgctgtt tgtggcgcag gggaatcagc
       61 tgttcatgaa cgatgtgttc ctgaagcgac tgtttgcagt gagcatcacc tcgtccggca
      121 atcccccgac gttctccctg acgccggagg gcaggctgac ggcccgcaat gcggacatca
      181 gtggagctgt cacagcgaat agcggcacgc tcaataatgt gaccattaac cagaactgcc
      241 ggatacttgg aaaactgtct gccaaccaga ttgaaggcga tattgttaaa accgtgggga
      301 aggctttccc gcgggactcc cgcgcaccgg agcggtggcc atcggggacc attaccgtca
      361 ggatttatga cgatcagcct tttgaccggc aaatagtgat cccggcagtg gctttcagcg
      421 gcgctaaaca tgagagagag cataacgata tttattcatc atgccggctg atggtgaaat
      481 gtaacggcag ggtgcttttt gaaaggacgg cgctggctga gtctcaggtg tattcagggg
      541 tgattgatat gcctgcagga agggggcata tgacgctgga gttttctgtg tctgcctggc
      601 tggtgaatgg atggtacccg acagcgagta tcagcgatct gctggttgtc gtgatgaaga
      661 agtccacggc gggtatcagt atttcgtgat catgaggagc cggattgccg gaaggagaca
      721 aaaaccgtac agtatgcgcg ggtgcctttg gctgatggcc ggagggaaca cctgaaggcc
      781 ggatgtgaaa aggccccgga caaacattca tgtttaaccc gaggcctgac cattcatcct
      841 aagcaagtg
//
LOCUS       DAZKFU020000629          846 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZKFU020000629 DAZKFU020000000
VERSION     DAZKFU020000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.639"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>846)
                     /locus_tag="ACHOQQ_RS26150"
                     /old_locus_tag="pgaptmp_005230"
     CDS             complement(<1..>846)
                     /locus_tag="ACHOQQ_RS26150"
                     /old_locus_tag="pgaptmp_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HGU4473331.1"
                     /translation="GVGQNGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTR
                     VNGDSDNMGQNIMSQLLSNSFVIIVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFK
                     FKKKGTEETVVEGDITVGLEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKY
                     TEINLADRIRSQFFIEYGIRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLL
                     VEYSDEVVSTLIKKPVIVNSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCL
                     AHNIN"
BASE COUNT          294 a          179 c          124 g          249 t
ORIGIN      
        1 tcattaatat tatgagccag acatacagat aaatgattat acatttcatc catggccgtg
       61 cgagtataac agccaatttt tgttaatttt tgagcatcac ttttcgtaac ccaataatat
      121 tgttcaccat tactatttac aatgacaggt ttcttgatca aagtcgaaac cacttcatct
      181 gaatattcaa ccaacaagac aagatcatga taaattttaa attgcgaagc tctgacttca
      241 tttagcatta atatgacatc ttcatcatta agaccttccc cctcacgaat gacaataccg
      301 ggaatgcgga taccatactc aataaaaaac tgactacgaa tcctatcagc aagattgatt
      361 tcagtgtatt ttttagcctg tacactattc ataatcaaga ccaagggtac agtttcggta
      421 ataacctgat caagtttatt aatgatacct aatgagatgt catcatcttg attatagggt
      481 tccaacccaa cggtaatatc tccctcaaca accgtttctt cagtcccttt ctttttgaat
      541 ttaaagtaaa agtaaattcc taagatcact gccaaacata aaaacacaag aaggggaaaa
      601 cctggaagta acccaatact tagcgcgagg acacatgtca caataatgac aaaagaatta
      661 cttaataact gggacattat attttgcccc atattatcac tatcgccatt tacgcgcgta
      721 acgataaatc ctgcgctaat agcaattaat aaagctggaa tttgagaaac aagaccatca
      781 ccgacagtaa gaattgtgta gacagtcaac gctgttgaga aatccattcc attttggcct
      841 actcct
//
LOCUS       DAZKFU020000630          842 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZKFU020000630 DAZKFU020000000
VERSION     DAZKFU020000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.640"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>842
                     /locus_tag="ACHOQQ_RS26155"
                     /old_locus_tag="pgaptmp_005231"
     CDS             <1..>842
                     /locus_tag="ACHOQQ_RS26155"
                     /old_locus_tag="pgaptmp_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473332.1"
                     /translation="SEAENQWVVQDTLKAYSEMDWLTAKLTSDETSPQIAGYMPEGAR
                     VEWNPADSNLNATGSNKRKYGLVTLKGLPEKNSSTLTPGQRYWVVVDNNNVKPAPGAG
                     PGWWRHLLPPAKEAMVFDKTVSLSSPFAIKVGDPIGHMGYYQAPKDGGYEARYQVHIE
                     CTSMDDNLEKFLTNPEQVGKNNPLWLKYAPGLALYKKDAFTGTFTKDARVTTRTGILP
                     LKQVQTEVDKSTKQEYWQLRPENAYVPKGQVEPQLLSQYDLAKLGFRTEIAEPTSFDY
                     LDGK"
BASE COUNT          256 a          188 c          230 g          168 t
ORIGIN      
        1 tctgaagcag aaaatcagtg ggtcgttcag gacactctaa aagcatattc tgaaatggac
       61 tggctgacgg cgaaactgac ttcagatgaa acttctccac aaattgcagg gtacatgcct
      121 gaaggggcca gagtggagtg gaacccggca gacagcaatc ttaacgctac aggaagtaac
      181 aagagaaaat acggtcttgt gacgctaaag ggtttaccag aaaaaaacag cagtactctc
      241 actcccggtc agcgctactg ggttgttgtg gataacaaca atgttaagcc agcgccgggg
      301 gccgggcccg gctggtggcg acacttgctg cctcccgcaa aggaagccat ggtattcgat
      361 aaaacagtaa gcctcagttc tccatttgcc attaaggtcg gtgacccgat tgggcacatg
      421 ggctactatc aggccccaaa ggatgggggg tatgaggcac gatatcaggt acatatcgaa
      481 tgcacgagta tggatgataa tctggagaaa ttcctgacca atcctgagca ggtgggaaaa
      541 aataatccac tctggctgaa gtacgccccg gggcttgcgt tgtacaagaa agatgctttt
      601 acaggaacat tcacgaagga cgcgagagtg acgaccagga cgggaattct gccactgaaa
      661 caggtgcaaa cagaagtcga taagtcgacc aaacaggaat actggcaatt gcggccagaa
      721 aacgcgtacg tgccaaaagg tcaggtggaa cctcaacttt tgtcacagta tgatctggca
      781 aagctggggt tcagaactga aatcgcagag cctaccagct ttgattacct ggatggaaaa
      841 aa
//
LOCUS       DAZKFU020000631          840 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZKFU020000631 DAZKFU020000000
VERSION     DAZKFU020000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.641"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>840)
                     /gene="espL1"
                     /locus_tag="ACHOQQ_RS26160"
                     /old_locus_tag="pgaptmp_005232"
     CDS             complement(<1..>840)
                     /gene="espL1"
                     /locus_tag="ACHOQQ_RS26160"
                     /old_locus_tag="pgaptmp_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HGU4473333.1"
                     /translation="FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQ
                     HVPLVRDAEFRALLRHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHT
                     LSVRLRIKQSAYGQLNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWA
                     DRYVRNCAIAILPLLPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFR
                     QLPILPEKEIIEIITAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNL
                     VKLM"
BASE COUNT          245 a          175 c          190 g          230 t
ORIGIN      
        1 cattaattta accagattat cttcatgaat gatgtgatta tctaccagtg actgaatttc
       61 ttgcataaat attttcacgt tgtcagtatg tccgttcatc atagccaaaa ataaagcagg
      121 tgtaccaacg ctattctgcg cagttattat ttcaatgatt tctttttcag ggagtatggg
      181 caactgtctg aataatgtaa taagctgttg agtctggcct gtggccatta ttaacaacat
      241 tgccgatgga tgtatagggg caaatggcat tcgtgtcgcg atacccgtga aaatagcccc
      301 tggaactccc tcaggtaata ggggcagaat agcaattgca cagttgcgaa catacctatc
      361 agcccaggtc tgagcatcgg gacctgaact gatgaactta tcaagcgaga gaaagccccg
      421 tgctgtcctg tgggtgcctc tgacggcaac gttggtatcg ttcgggtcgt aaacggacac
      481 aacataattt aattgcccat acgccgactg ttttattcgt agccttactg acaaagtatg
      541 attgtctata tataaaatcg cagacatatg actgacattg tttgccatca ttcgacagaa
      601 gaaaatatct aaagcgaagc caaaatcctt actcgcgata acgcgactcc ctgcaggagg
      661 atggcgaagt aatgctctga actccgcgtc ccggactaag gggacatgct gctgtatgga
      721 ttcaacggag gagtaatgtt taatcaggtt gaccttgcct tttccatttc tcaatgaatc
      781 aagtgcgtat tgcaatgaca gatggcgaca aaaaatagcg tcagaaggag attgcgaaaa
//
LOCUS       DAZKFU020000632          836 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZKFU020000632 DAZKFU020000000
VERSION     DAZKFU020000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.642"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..436)
                     /locus_tag="ACHOQQ_RS26165"
                     /old_locus_tag="pgaptmp_005233"
     CDS             complement(<1..436)
                     /locus_tag="ACHOQQ_RS26165"
                     /old_locus_tag="pgaptmp_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473334.1"
                     /translation="MSSSVKYSPVPAVLTRPESLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFCQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQRVPGDSVNCMCWASLAALAFDAEERVE"
     gene            complement(421..>836)
                     /locus_tag="ACHOQQ_RS26170"
                     /old_locus_tag="pgaptmp_005234"
     CDS             complement(421..>836)
                     /locus_tag="ACHOQQ_RS26170"
                     /old_locus_tag="pgaptmp_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473335.1"
                     /translation="PEGLPLLESGGGNGGVFYDNEPLLLIASRRLRASLETALIVLQK
                     WTVCVGCGYSGVRDGAGKNAPVWVKVNPESGLGLCSGCDSFLLQERQRRQRHDQLFSC
                     GLSCKQRKTEGAVYLRGSDAPGGLRDMEVKVCLPQ"
BASE COUNT          211 a          279 c          199 g          147 t
ORIGIN      
        1 gttccacccg ctcttcagca tcaaatgcca gtgcagcaag gcttgcccag cacatgcagt
       61 tcactgaatc ccccggaacg cgctgcagct cccgacgcac catgttgacc tccgcactga
      121 aaaaagcgtt ctcatcgcct tcaagggcag ccagatgcag cggaagccag acctgacaga
      181 accattcaca catccgttcc cggttactga cggtcatcac tgtctccggg cgggaaccgt
      241 cctgaaaaac atgtccggtg cgtggagaac tgtatgaaac ataaccaccg tgcaccgcaa
      301 tgaccggacg acctgacgcc ggaagcccca cacgacaccc actggcaatc cgggtcatca
      361 gcaggttcag ttcagccagc gattcagggc gggttaagac cgccggaacc ggagaatatt
      421 tcactgagga agacatactt tcacctccat atctctcagc ccgccaggtg cgtcacttcc
      481 ccgcagatat accgccccct ccgtctttcg ctgtttgcag gaaagaccac atgaaaaaag
      541 ctggtcatgc cgctgacgac gctggcgttc ctgcaaaaga aaggagtcac aaccggaaca
      601 cagccccaga ccactctcag gattcacctt cacccagacc ggagcattct taccggcacc
      661 atcccgtacc ccggaataac cacagcccac gcacaccgtc cacttctgca gtacgatgag
      721 tgcagtttcg agtgatgcgc ggagacgccg ggaggcaata aggagcaacg gctcattgtc
      781 ataaaaaacc ccgccgttac cccctccgga ttcaagcagc gggagtcctt caggct
//
LOCUS       DAZKFU020000633          832 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZKFU020000633 DAZKFU020000000
VERSION     DAZKFU020000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.643"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>832
                     /locus_tag="ACHOQQ_RS26175"
                     /old_locus_tag="pgaptmp_005235"
     CDS             <1..>832
                     /locus_tag="ACHOQQ_RS26175"
                     /old_locus_tag="pgaptmp_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4473336.1"
                     /translation="SGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSMAGSNAAYELTS
                     TYTPAGQLQSQHLNSLVYDRDYGWSDNGDLVRISAPRQTREYGYSATGRLESVRTLAP
                     DLDIRIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRHDEYGRLTEKTDR
                     IPAGVIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRE
                     RDLTGWMSLSRKPEVTWYDWDGDRLTTVQTDTTRIQTVYEPGSFTPLIRVETENGERE
                     K"
BASE COUNT          187 a          254 c          285 g          106 t
ORIGIN      
        1 agcggttacc tggcgggcat gaagctgggc gggacgccgc tggtcgagta tacgcgggac
       61 aggctgcacc gtgagacggt gcgcagcttc ggcagcatgg caggcagtaa tgccgcatac
      121 gaactgacca gcacatacac ccccgcaggc cagttacaga gccagcacct gaacagcctg
      181 gtatatgacc gtgactacgg gtggagtgac aacggcgacc tggtgcgcat cagcgccccc
      241 cggcagacgc gggaatacgg ctacagcgcc acgggcaggc tggagagcgt gcgcaccctc
      301 gcaccggacc tggacatccg catcccgtat gcgaccgacc cggcgggaaa ccggctgccg
      361 gacccggagc tacacccgga cagcacgctc acggcgtggc cggataaccg catcgcggag
      421 gatgcgcact atgtctaccg acacgatgaa tacggcaggc tgacggagaa gacggaccgc
      481 atcccggcgg gtgtgatacg gacggacgac gagcggaccc accactacca ctacgacagc
      541 cagcaccgcc tggtgttcta cacgcggata cagcatggcg agccactggt cgagagccgc
      601 tacctctacg acccgctggg acggcgaatg gcaaaacggg tctggcggcg ggagcgtgac
      661 ctgacggggt ggatgtcgct gtcgcgtaaa ccggaggtga cgtggtatga ctgggacgga
      721 gacaggctga cgacggtgca gactgacacc acacgtatcc agacggtata cgagccggga
      781 agcttcacgc cgctcatccg ggtcgagaca gagaacggtg agcgggagaa ag
//
LOCUS       DAZKFU020000634          832 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZKFU020000634 DAZKFU020000000
VERSION     DAZKFU020000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.644"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            79..>832
                     /locus_tag="ACHOQQ_RS26180"
                     /old_locus_tag="pgaptmp_005236"
     CDS             79..>832
                     /locus_tag="ACHOQQ_RS26180"
                     /old_locus_tag="pgaptmp_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbaSI family restriction endonuclease"
                     /protein_id="HGU4473337.1"
                     /translation="MDKTDYILRSLTKTSKKRWEHYAVTRIYHLLNDPDIEFVCQQCV
                     RKENGKIYLADLFFPQLELYLEIDEGHHNSEQSQIDDAKRRMDITEVTGFLEKRIPAS
                     EISLEIFDRYINEFIELIRNRKNRMIKDKKFKEWDYDNKYTARPHLKAGRISVGPDAV
                     FRYHRDALNCFGYNKGHHQSGGWSIPSHISEAMGLSGKCMVWFPKLYGNSDWGNELTD
                     DGNKIIERNIKDDEGYTERWDKRIVMAHSRDEL"
BASE COUNT          302 a          132 c          174 g          224 t
ORIGIN      
        1 gcatcttatg gcgataggta acactatcgc cataagaaaa agcacattac atgaagaaca
       61 aagaaggaag gcatataaat ggataaaact gattacatcc tacgttcact tacgaaaacc
      121 tcaaaaaaac gatgggaaca ttatgcagta actcgcattt atcatctttt aaatgatccc
      181 gacattgaat ttgtctgcca gcaatgtgtc agaaaagaaa atggaaaaat ctatttggca
      241 gatttatttt tcccacaact ggaactttat cttgaaattg atgaagggca tcacaacagt
      301 gagcaatcac aaatagatga tgcgaaacgt cgtatggata ttactgaagt tacaggattt
      361 ttagagaaaa gaattcccgc cagcgaaata agtcttgaaa ttttcgatcg ttatatcaat
      421 gaatttattg agttgatacg caatcgtaaa aatagaatga ttaaagataa aaaattcaaa
      481 gaatgggatt atgataataa atatacagct cgtcctcatc ttaaggcagg aaggatatct
      541 gtaggtccag atgccgtctt cagataccac cgggatgctt tgaactgttt cggttacaac
      601 aaaggacatc atcagtctgg cggttggagc attccttctc acataagtga agcaatgggt
      661 ctatcaggaa aatgcatggt ttggtttcca aaactatatg gaaattcaga ttggggaaat
      721 gaattgactg atgatggtaa caaaatcata gagcggaata taaaggatga tgagggatac
      781 acagaacgct gggataaacg aattgttatg gcacattctc gtgatgagtt ga
//
LOCUS       DAZKFU020000635          818 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZKFU020000635 DAZKFU020000000
VERSION     DAZKFU020000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.645"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..278
                     /locus_tag="ACHOQQ_RS26185"
                     /old_locus_tag="pgaptmp_005237"
     CDS             51..278
                     /locus_tag="ACHOQQ_RS26185"
                     /old_locus_tag="pgaptmp_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HGU4473338.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
     gene            655..>818
                     /locus_tag="ACHOQQ_RS26190"
                     /old_locus_tag="pgaptmp_005238"
     CDS             655..>818
                     /locus_tag="ACHOQQ_RS26190"
                     /old_locus_tag="pgaptmp_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible protein OsmY"
                     /protein_id="HGU4473339.1"
                     /translation="MRLLYSAVVLTSALLLQGCVAAAVVGTAAVGTKAATDPRSVGAQ
                     VDDEVLELRV"
BASE COUNT          262 a          139 c          194 g          223 t
ORIGIN      
        1 tttaaagttt atatgtactg ttacagtgtt gatgaaagga gataaaaaca atgttcccgg
       61 agtacagaga actgatttca gcattgaaag gaaatcataa acgctttgat tctttatttg
      121 aaaagcataa taaacttgat cacgaaataa aaagaattga agatgaattt ggttcagcgt
      181 attcgacgaa agtggtcagg ctgaagcttg aaaaacttga gatcaaaaga gaaatccaga
      241 aaatccttga agatgagtca gaaaagggcg aacggtaatc ataaacggta tattcaaaga
      301 cgtgttttct ctgttttatg aaaaaggcac cgggggctcc acggtgcctt ttagcctgtg
      361 tatagcgaaa aggagatcct gttcagcgaa ccagatagtt aaaaaaagaa tctttacgac
      421 ggtatcaaag ttttcgtgca aaaaacatga tcaatattaa attccagtac atccatatcc
      481 gatataaatc tttccgtctg acagatacag atatgccacg taacgcatcc gctgttccgg
      541 ttgtgccgga ggataataac agtctgtagc ttattttgtt tcactgaagg taaacttttt
      601 gtacgataaa ttccgggaat atgaagtaaa aatcaaaaca attccggggg agatatgcgt
      661 ttattatatt ctgctgttgt ccttacttca gccttattgt tacaggggtg cgtggctgcc
      721 gctgtggtgg gtacagctgc tgtcgggaca aaggcagcaa ccgacccacg cagtgtcgga
      781 gcgcaggtgg atgatgaagt gctggagctg agggtcag
//
LOCUS       DAZKFU020000636          817 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZKFU020000636 DAZKFU020000000
VERSION     DAZKFU020000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.646"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>817)
                     /locus_tag="ACHOQQ_RS26195"
                     /old_locus_tag="pgaptmp_005239"
     CDS             complement(<1..>817)
                     /locus_tag="ACHOQQ_RS26195"
                     /old_locus_tag="pgaptmp_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4473340.1"
                     /translation="SGYLAGMKLGGTPLLEFTRDRLHRETVRSFGNNAYELTSTYTPT
                     GQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIR
                     IPYATDPAGNRLPDPELHPDSTLTVWPDNRITEDAHYLYRYDEYGRLTEKTDRIRAGV
                     IRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLCDPLGRRMAKRVWRRERDLTG
                     WMSLSRKPEETWYGWDGDRLTTVQTDTTRIQTVYEPGSFTPLIRVETENGERKK"
BASE COUNT          112 a          270 c          250 g          185 t
ORIGIN      
        1 cttttttccg ttcgccgttc tctgtctcga cccggatgag cggcgtgaag cttcccggct
       61 cgtataccgt ctggatacgt gtggtgtcag tctgcaccgt cgtcagcctg tcgccgtccc
      121 atccgtacca tgtctcctcc ggtttacgcg acagcgacat ccaccccgtc aggtcacgct
      181 cccgccgcca gacccgtttt gccattcgcc gtcccagcgg gtcacagagg tagcggctct
      241 cgaccagtgg ctcaccatgc tgtatccgag tataaaacac caggcggtgc tgactgtcgt
      301 agtggtagtg gtgggtccgc tcgtcgtccg tccggataac ccctgcccgg atgcggtccg
      361 tcttctccgt cagcctgccg tactcatcgt agcggtacag gtagtgcgcg tcttcggtga
      421 tgcggttatc cggccacacc gtgagcgtgc tgtcaggatg cagctccggg tccggcaggc
      481 ggttgcccgc cgggtcggtg gcatacggga tgcggatgtc caggtccggt gcgagggtgc
      541 gcacgctctc cagcctgccc gtggcactgt agccgtattc ccgcgtctgt cgcgggccgc
      601 tgatgcgcac caggtcgccg ttgtcattcc acccgtagtc acggtcatac accaggctgt
      661 tcaggtgctg gctctgtaac tggcctgtgg gggtgtaagt gctggtcagt tcatatgcat
      721 tattgccgaa gctgcgcacc gtctcccggt gcaggcggtc gcgcgtgaac tccagcagcg
      781 gcgtcccgcc cagcttcatg cccgccaggt aaccgct
//
LOCUS       DAZKFU020000637          811 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZKFU020000637 DAZKFU020000000
VERSION     DAZKFU020000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.647"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            348..>811
                     /locus_tag="ACHOQQ_RS26200"
                     /old_locus_tag="pgaptmp_005240"
     CDS             348..>811
                     /locus_tag="ACHOQQ_RS26200"
                     /old_locus_tag="pgaptmp_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473341.1"
                     /translation="MCDFIKSCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTSPTKENEPVFFSHGSHIHSYFICSPFFLVH"
BASE COUNT          284 a          150 c          129 g          248 t
ORIGIN      
        1 ataatgcatt ctgagcatat cagagcgcat aaaaggcgtc aattatccat gctccctcac
       61 gaaacagaag ctgaactctt aacacctgct atcggaactc actgaataaa caactaaaaa
      121 ggacattgtg aaaattaaca tcaacagtat gtaaagtgat ttgttttgtc ttaaatgggt
      181 gaatgtaaaa aaatattcta tacaatccac acatccttgt atcatatatc aagtaaacat
      241 ccacagagat aacagcttat ttcaaataag cagattctgt ttgatacttc agaaaaatag
      301 tatatgctcc tgatttactg atgttataag ttatacagga tagttaaatg tgtgatttta
      361 taaaatcatg ttatgcagca ccaagaatta acaaaatggc tgattttttc tcatcaacag
      421 catctggagt aaaacataaa cctttgaagc atgtaaaaat tgataacagg cttttcccat
      481 ctcctgagta tataattttt tctgactatc ttacgaaaaa cagtggtcca tttgttaacc
      541 actacttttg cagtattcca tacagcaaag aagaagaatg caggctgggt gtgactatta
      601 tgaaatatgc aagccatcga aataagccat tgagcttatg gtgtcttgga atggcagaag
      661 gagccatggc cagaacaatt tctcaactat caaaaggaca ggttttatca ttaacaacca
      721 gcccaacaaa agaaaatgaa cctgtattct ttagtcatgg ttctcatatt cactcttact
      781 ttatttgctc cccattcttt cttgttcatg a
//
LOCUS       DAZKFU020000638          802 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZKFU020000638 DAZKFU020000000
VERSION     DAZKFU020000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.648"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            50..>802
                     /locus_tag="ACHOQQ_RS26205"
                     /old_locus_tag="pgaptmp_005241"
     CDS             50..>802
                     /locus_tag="ACHOQQ_RS26205"
                     /old_locus_tag="pgaptmp_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HGU4473342.1"
                     /translation="MVLLNGNTVYSPLKELVKMPDFSVSLDREQDKNTLQELNNRVIS
                     LSMTDNRGFEADRVTLVLDDADGQLALPGRGTHIRVMLGWKGEPLVDKGTYIVDEVAH
                     EGPPDRLTLSANSADFREEFNVKREVSWHDVTVERVVSAIAHRYGLKAQISDMLMDIE
                     IDHADQTEESDISFLTRMADMLGAITTVKNGCLLFILPGGGVTADGRPLPSFALARSD
                     GDSHQFRIADRQAYTGVKAYWLDLNHGKKKAVN"
BASE COUNT          209 a          190 c          232 g          171 t
ORIGIN      
        1 tccggtggtg tttctggtat gcaactgtgg cgggaaaaac ggaggaatta tggtattgct
       61 gaacggtaac acggtttatt cccccctgaa agaactggtg aaaatgcctg atttcagcgt
      121 ttcactggac agggagcagg ataaaaacac acttcaggaa ctgaataacc gggtgatatc
      181 gctttccatg acagacaacc gggggtttga agcagacagg gtcactcttg tgctggatga
      241 tgccgacggg cagctggctc tccccgggcg gggaacacac atcagagtca tgctgggatg
      301 gaagggagag cctctggttg ataaaggcac ctacattgtt gatgaggtcg ctcatgaagg
      361 acctccggat cggctgacac tgagtgcaaa cagcgcagac ttcagggagg aatttaacgt
      421 caaacgggaa gtatcctggc acgacgtgac tgttgaacgc gtggtttccg ctattgccca
      481 ccgttacgga ctgaaagccc agataagcga tatgcttatg gatattgaaa tcgatcatgc
      541 cgaccagacg gaagaaagcg acatatcatt ccttacccgc atggctgata tgctgggagc
      601 catcactacc gtcaaaaatg gctgtctgct gttcattctg ccgggtggcg gcgtgacagc
      661 tgacgggcgc ccactcccct catttgcact ggcacgcagt gacggagaca gtcaccagtt
      721 cagaatagcc gatcgccagg catacacagg ggtgaaggcg tactggctgg atttgaatca
      781 tggcaaaaag aaagccgtca ac
//
LOCUS       DAZKFU020000639          800 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZKFU020000639 DAZKFU020000000
VERSION     DAZKFU020000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.649"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..414)
                     /locus_tag="ACHOQQ_RS26210"
                     /old_locus_tag="pgaptmp_005242"
     CDS             complement(<1..414)
                     /locus_tag="ACHOQQ_RS26210"
                     /old_locus_tag="pgaptmp_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473343.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLNGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MLAVVLFLGALFMEGVSSVILFRGCGSAAVSGEGRG"
     gene            complement(411..>800)
                     /locus_tag="ACHOQQ_RS26215"
                     /old_locus_tag="pgaptmp_005243"
     CDS             complement(411..>800)
                     /locus_tag="ACHOQQ_RS26215"
                     /old_locus_tag="pgaptmp_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4473344.1"
                     /translation="DARRLKYLRKISPYVFEELLLLAFEQQGMPVIRNPSYSGDGGLD
                     GQVVINGERWLIQAKRYRRTIRPAHIDAFGELLRRKGCRGFFIHTGRTGNISRARLTL
                     WPDIELISGQRLLQLLNGTLLSNKGES"
BASE COUNT          214 a          253 c          166 g          167 t
ORIGIN      
        1 ccccctgcct tccccggata cggctgcaga accacaacca cgaaacagaa tgactgacga
       61 gacaccttcc ataaacaaag cgcccagaaa cagaaccacc gccagcattt tcagttcacg
      121 aatatcttca gcaacagcct ccccgaaaga aatcgctgtt cgcggacatt cctgctcatc
      181 gacatacatc cctgagacgg atatgacgga ggaacatttt tgtaccatca catgcccttc
      241 aggcgcatca cgccagcggg atgactccgt cgcataacgc tgtgtcatgc ctgcctcacc
      301 accattcaga ttgttccaga catacagcat taccagacat cctgccagca ttgacggcca
      361 gacaatcaga agccggagca gcagcataac aaaccggtac agtgtcataa tcatgattct
      421 cctttgttac tcagtaaggt gccattcagc agttgcaaca gacgttgtcc actgatcagc
      481 tcaatatccg gccacagtgt cagccgtgcc cggctgatgt tccccgtacg ccccgtatgg
      541 ataaaaaagc ccctgcatcc cttccggcgc agcagttcgc caaaagcatc aatatgtgcc
      601 ggacgaatgg ttcttctgta acgtttagcc tgtatcagcc agcgttcacc gttaatcacc
      661 acctgaccat caagaccacc atcgccgctg tatgaggggt tacggataac cggcatcccc
      721 tgttgctcaa aagccagcag caacagttcc tcaaacacat acggactgat tttgcgcaga
      781 tatttcagcc gccgggcatc
//
LOCUS       DAZKFU020000640          800 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZKFU020000640 DAZKFU020000000
VERSION     DAZKFU020000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.650"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..414)
                     /locus_tag="ACHOQQ_RS26220"
                     /old_locus_tag="pgaptmp_005244"
     CDS             complement(<1..414)
                     /locus_tag="ACHOQQ_RS26220"
                     /old_locus_tag="pgaptmp_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473345.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCPSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRG"
     gene            complement(411..>800)
                     /locus_tag="ACHOQQ_RS26225"
                     /old_locus_tag="pgaptmp_005245"
     CDS             complement(411..>800)
                     /locus_tag="ACHOQQ_RS26225"
                     /old_locus_tag="pgaptmp_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4473346.1"
                     /translation="DARRLKYLRKISPYVFEELLLLAFEQQGMPVIRNPSYSGDGGLD
                     GQVVIGGECWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRTGNISRARLTL
                     WPDIELISGQRLLQLLNGTLLSNKGES"
BASE COUNT          211 a          254 c          167 g          168 t
ORIGIN      
        1 ccccctgcct tccccggata cggctgcaga accacaacca cgaaacagaa tgactgacga
       61 gacaccttcc ataaacaaag cgcccagcaa cagaaccact gcctgcattt tcagttcacg
      121 aatatcttca gcaacagcct ccccgaaaga aatcgctgtt cgcggacatt cctgctcatc
      181 gacatacata cctgagacgg atatgactga gggacatttt tgtaccatca catgcccttc
      241 aggcgcatca cgccagcggg atgactccgt cgcataacgc tgtgtcatgc ctgcctcacc
      301 accactcaga ttgttccaga catacagcat taccagacat cctgccaaca ttgacggcca
      361 gacaatcaga agccggagca gcagcataac aaaccggtac agtgtcataa tcatgattct
      421 cctttgttac tcagtaaggt gccattcagc agttgcaaca gacgttgtcc actgatcagc
      481 tcaatatccg gccacagtgt cagccgtgcc cggctgatgt tccccgtacg acccgtatgg
      541 ataaaaaatc ccctgcatcc cttccggcgc agcagttcgc caaacgcgtc aatgtgtgcg
      601 ggacgaatgg ttcttccgta acgtttggcc tgtatcagcc agcactcacc gccaatcacc
      661 acctgaccat caagaccacc atcgccgctg tatgaggggt tacggatcac aggcattccc
      721 tgttgttcaa aagccagcag caacagttcc tcaaacacat acggactgat tttgcgcaga
      781 tatttcagcc gccgggcatc
//
LOCUS       DAZKFU020000641          794 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZKFU020000641 DAZKFU020000000
VERSION     DAZKFU020000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.651"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>794)
                     /gene="espY3"
                     /locus_tag="ACHOQQ_RS26230"
                     /old_locus_tag="pgaptmp_005246"
     CDS             complement(<1..>794)
                     /gene="espY3"
                     /locus_tag="ACHOQQ_RS26230"
                     /old_locus_tag="pgaptmp_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HGU4473347.1"
                     /translation="ATSDLDSYLKLDFDRLSPKQKQTTLCCFWNKIASSLPEPYNSTI
                     KHNIIFYKDGENLMIRGTISIVNEVVKTYSLPIEKDDNGYYDFSGLYLAHSNISGKDP
                     NKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNANCTNANFNNCRFKKCDLTNMNCT
                     GAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKSDGNETKGMILTNCSCVKTTFNWA
                     DLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYRTDLFNPILKTE"
BASE COUNT          221 a          142 c          145 g          286 t
ORIGIN      
        1 ttctgttttt aatatcggat taaaaaggtc ggtacggtaa agtttcgtgc ctttcatcct
       61 gacgatgtca ggaagaatag tgtttgaaag attagcctcg ctaaagtcca cattttggca
      121 atcactttct gataaatctg cccaattgaa agtggtttta acacaggagc agttagttag
      181 aatcatccct ttcgtttcat tcccatctga tttctgataa gttatccttt gtattatttg
      241 atctgcttct ggatattgca tctcaggttc tttttcttta ccatatatca tcgcattatc
      301 aagaattgcg ccagtgcaat tcatatttgt caggtcgcat ttcttaaatc tacaattgtt
      361 aaaattcgca tttgtacaat tcgcatttgt aaacttgacg ccataaaaat aagtatgttc
      421 gaagttgaca tttgagcagt tacagtttcc catatctata ccgaaatcta tatcgggatc
      481 tttattggga tctttaccgg aaatattgct gtgtgctaga tatagaccgc tgaaatcgta
      541 atatccattg tcatcctttt ctattgggag tgaataagtc tttactacct cgttaacaat
      601 agagatagtt ccacgtatca tcagattttc tccgtcttta taaaaaataa tgttatgttt
      661 gatggtactg ttgtatggct ctggcaatga ggatgcaatt ttattccaga aacaacaaag
      721 cgtggtctgc ttttgtttgg gcgagaggcg atcaaaatcc agctttaaat atgagtctaa
      781 atctgacgtt gcat
//
LOCUS       DAZKFU020000642          788 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZKFU020000642 DAZKFU020000000
VERSION     DAZKFU020000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.652"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>788)
                     /locus_tag="ACHOQQ_RS26235"
                     /old_locus_tag="pgaptmp_005247"
     CDS             complement(<1..>788)
                     /locus_tag="ACHOQQ_RS26235"
                     /old_locus_tag="pgaptmp_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4473348.1"
                     /translation="TEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRH
                     RYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDPLGLDLLSLPSPRQKPVSQSCT
                     IDPINQQPVGRFIGDSKGNIMVEPVGGRTIPYPPSNPGSADTHTLYQNNSNAYRLNPI
                     GHANNSTPHAHAHLPGTGSGMKGQGPSLDVNGRVVPWNSSAAHIGIRSLGTLSFFSSV
                     LHMFTLRSCREGTFGQCYCAMYVEMSTGSNQDEADIMCGMPEYF"
BASE COUNT          215 a          184 c          162 g          227 t
ORIGIN      
        1 aaaatactca ggcattccgc acattatatc agcctcatct tgattgcttc ccgttgacat
       61 ttcaacatac atagcacaat aacattgtcc aaacgttccc tctctacatg atctaagtgt
      121 aaacatatgt aaaacagaac taaaaaagct tagtgtaccg agtgatctaa taccgatatg
      181 tgcggcacta ctattccatg gtacaacgcg accattaaca tcaagggatg gaccttggcc
      241 tttcataccg ctacctgttc ctggtaaatg tgcatgtgca tgtggtgttg aattgtttgc
      301 atgccctatt ggattaagcc tataagcatt tgaattattc tgataaagag tgtgagtatc
      361 tgcacttcct ggattactgg ggggatacgg gattgttctg ccacctaccg gttcaaccat
      421 aatattgcct ttagaatcac ctataaacct accaacaggt tgttgattta taggatcaat
      481 agtacaggat tgacttacag gtttttgtct gggagaaggt aatgataata gatctaatcc
      541 caaagggtct atatactgaa ccggattcaa cggatactga taaaaattcc atcccccctt
      601 cagtccaatc ggatcctgag tgatatacct cccctgcagc gggtcataat agcggtggcg
      661 gttgtaatac aggccggact cctcatcata ctgctgcccc ggcaggcgga taagctgctg
      721 cagctgatgc gggttctctt cattcagcag gttgccccac tcatcgtatt ctgcgcacca
      781 ttctgtag
//
LOCUS       DAZKFU020000643          787 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZKFU020000643 DAZKFU020000000
VERSION     DAZKFU020000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.653"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..309)
                     /locus_tag="ACHOQQ_RS26240"
                     /old_locus_tag="pgaptmp_005248"
     CDS             complement(<1..309)
                     /locus_tag="ACHOQQ_RS26240"
                     /old_locus_tag="pgaptmp_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HGU4473349.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     I"
     gene            complement(326..>787)
                     /locus_tag="ACHOQQ_RS26245"
                     /old_locus_tag="pgaptmp_005249"
     CDS             complement(326..>787)
                     /locus_tag="ACHOQQ_RS26245"
                     /old_locus_tag="pgaptmp_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4473350.1"
                     /translation="TDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGN
                     NGNNMQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRD
                     GFLTHSCLNAFGPSLGPRFCFEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          239 a          159 c          134 g          255 t
ORIGIN      
        1 gatctttccc ttacttaata aatagcatgt ataggattcg gaaagtccag catctatttt
       61 gtacctcatt atattagata aggatgtgag aaattccttg ttatcattcg cagccatata
      121 tgccagtccg agttcagcct ctgcggcctt acaggcaaaa tagctaagtg aacacgcttt
      181 tcgtccctct ttagtactgg ctgatattag tgcccataat attgctttgt ctgccaaatc
      241 taactctctc tgaaattgtc ttgcagtaac tgcactcgca gaaatagaga aaaaaataca
      301 attgttaata accttgccat attttttagt ccgtaactgt taatgtgcta tctggctctg
      361 aaaatattaa ctcatttaat ttttgcatgt cattagatga accagttata caaccctcga
      421 aacagaatcg aggtcctaaa gacggaccga aagcgtttaa acaactatgt gttaaaaatc
      481 catcacgatt atatgtttct gtatttgctg acggtactaa acgtatacca ttaggttttc
      541 tattggttgc tccaggacca ttaacattcc agctccatac cccttgaggt attggacctc
      601 tattttgtaa gtgtgtacaa ccaggattat ttttacattg catattatta ccattattac
      661 cagaggcgac aggaatagta agcacatcat aagagttatc atcattttgc gtatctgaaa
      721 cgcatgaaag catacttaaa gttatgctgt ataaacaggt cgctaatccc aacgggtcaa
      781 tatcagt
//
LOCUS       DAZKFU020000644          781 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZKFU020000644 DAZKFU020000000
VERSION     DAZKFU020000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.654"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(18..614)
                     /gene="fimE"
                     /locus_tag="ACHOQQ_RS26250"
                     /old_locus_tag="pgaptmp_005250"
     CDS             complement(18..614)
                     /gene="fimE"
                     /locus_tag="ACHOQQ_RS26250"
                     /old_locus_tag="pgaptmp_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGU4473351.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          245 a          188 c          147 g          201 t
ORIGIN      
        1 attatcaatg agttaaatca aacctcttct ctttttaatt tttcgtttat gagattattt
       61 ctttcccata atccggcaaa acgagcagca ttactggcgg tataacgtac agtatggcga
      121 atatttcgat gcccgagata atcctgaatt aaacgagtat ctgcaccacg ctccgccagt
      181 tcataaccgc aagcatgcct taacatatga ggatgagtct gcgttacggt tccagcttca
      241 ataccggcat cacgaataat gcgataggcc tgctggcgag aaagccgact cccgcggcga
      301 gaaataaata tggcgtcggt ccggtcagcg cctttccagt tagcacgttc cagggtccag
      361 cgttccacag cttcacgctc atcaaaacgt aacgggtgaa cggtagaaaa tccgttcttc
      421 agtcggcgaa tatttattcg accttcatta aggtcaagat cctgataatg cagatcaagc
      481 agttcactaa tacgcatccc atgccgatat gccaacagaa taagacaata atctctggct
      541 cccgttgccc cgtaacaaac cgcctgcatc atggcctgaa cttctttacc ggtaagataa
      601 cgacgtttac tcacaatagt agtactccta actgagatat atttgaatga atacctatag
      661 gaaacctcaa tcggtcaaaa ttagcccaat agataaaaag ataaccacac acgaagtgat
      721 gtggctatca gtcaattaca ttaaatatca tacaaaaata aaatatcact gatgtgacaa
      781 c
//
LOCUS       DAZKFU020000645          778 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZKFU020000645 DAZKFU020000000
VERSION     DAZKFU020000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.655"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..722
                     /locus_tag="ACHOQQ_RS26255"
                     /old_locus_tag="pgaptmp_005251"
     CDS             3..722
                     /locus_tag="ACHOQQ_RS26255"
                     /old_locus_tag="pgaptmp_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4473352.1"
                     /translation="MTKKITITDKDIQNAERLRKIWDEKRKQLSLSQEKAADILGFKT
                     QGAVSQLLNAKIALNTENTLKFAALLQVPAEEINPDLSDLLRSIRTHTPGNRRNLFEK
                     YYEYPLLSCVQAGAFSMDDFSYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGK
                     PSFPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIEC
                     NENCQFVGKVIKSQWNDETFD"
BASE COUNT          241 a          170 c          185 g          182 t
ORIGIN      
        1 ctatgacgaa gaaaattaca attactgaca aagacatcca gaacgctgaa cgactgcgca
       61 aaatctggga tgaaaaacgg aaacagctat ccctaagcca ggaaaaagcc gcagatatcc
      121 tcggcttcaa aacgcagggg gctgttagtc agttattgaa cgcaaaaatt gcgttaaata
      181 ctgaaaatac ccttaagttt gcggctttat tacaggttcc tgcggaagaa attaatccag
      241 atctgagtga tttgttacgc agtattcgta cccacacacc gggaaacagg cgtaacctgt
      301 tcgagaaata ttacgaatat ccgttactct cctgcgtaca agctggagct ttctcaatgg
      361 atgatttttc gtacaccgca aaggatgcga ttaagtggat ctccaccacc acaaaagcca
      421 gtgacagatc gttctggctg gaggtcaagg ggcattcaat gaccgcgcca cagggaggta
      481 aacccagctt ccctgaagga atgctgatac ttgttgaccc tgaacgggaa atcgaggatg
      541 gcgatttttg cgtggcccga atgaacggcg atgaattcac cttcaaacga ttcattcgtg
      601 agagcggtaa agcgtacctg gagccactca acccacgatt cgacatgatt gagtgtaacg
      661 aaaactgcca gtttgtagga aaagtcataa agtcgcagtg gaatgatgag acttttgatt
      721 gaattcacag aacaatttaa catccatcac gtcctttggg gcgtggagga tgttaaac
//
LOCUS       DAZKFU020000646          775 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZKFU020000646 DAZKFU020000000
VERSION     DAZKFU020000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.656"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..641)
                     /locus_tag="ACHOQQ_RS26260"
                     /old_locus_tag="pgaptmp_005252"
     CDS             complement(<1..641)
                     /locus_tag="ACHOQQ_RS26260"
                     /old_locus_tag="pgaptmp_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473353.1"
                     /translation="MMAAKYKTIITNAGTAALAAAVLPDGEKITLTHIAVGDGNGKPT
                     EPLATQTALVKEVWRGEVSRVSRDAENTSFLTAEVIIPANEGGFWIREIGLFDSKGTL
                     IAVGNVAEGYKPQLDEGSGREQVFRMVILLSNEASVNLTADSSTTMATQAGVAEMIKQ
                     HEQNGKHPDATTTSKGFTQLSSETDSDDETRAATPKAIKALKALAVTRPTAAL"
BASE COUNT          127 a          206 c          206 g          236 t
ORIGIN      
        1 ctgagggcgg ctgttggtct tgttactgcc agcgccttaa gtgcttttat tgctttgggt
       61 gtggctgccc gggtttcatc atcactgtcg gtttcactac tgagctgagt aaatccctta
      121 ctggtggttg tggcatccgg gtgtttaccg ttttgttcat gctgctttat catttctgcc
      181 acgccagcct gtgttgccat ggtggttgat gagtcggctg tcagatttac agaggcttcg
      241 ttgctcagca gaatcaccat gcggaatacc tgttctctgc ctgagccctc atcaagctgc
      301 ggtttgtaac cttccgcaac attacccacg gcgatgagtg tgcctttgct gtcaaacaga
      361 ccgatttcgc ggatccagaa tcctccttca ttcgcaggaa tgatcacttc ggcggttaaa
      421 aaactggtgt tttccgcgtc acggctgacc ctgctgacct cgccccgcca gacctcttta
      481 acgagagcgg tttgcgtggc cagtggttct gtgggtttac cgttaccgtc tccgacggcg
      541 atatgtgtca gcgtgatctt ttctccatcc ggtaacaccg cagctgcgag ggctgctgtt
      601 ccggcatttg tgataatggt tttgtatttt gcggccatca ttactccttg tccggtctga
      661 tggttactgt gatgccttca taggtgactc cacccgggtg gatgtcacca ctgactgaca
      721 gcgggcttgt cagttctgct gccggatata ccacgatggt ttccccggtc agcgt
//
LOCUS       DAZKFU020000647          770 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZKFU020000647 DAZKFU020000000
VERSION     DAZKFU020000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.657"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..641)
                     /locus_tag="ACHOQQ_RS26265"
                     /old_locus_tag="pgaptmp_005253"
     CDS             complement(<1..641)
                     /locus_tag="ACHOQQ_RS26265"
                     /old_locus_tag="pgaptmp_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HGU4473354.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAPH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKT"
BASE COUNT          179 a          234 c          203 g          154 t
ORIGIN      
        1 gttttcccca ttgacggacg ggcagccagc aggaccaggt cacctggctg cagaccacag
       61 gtcatttcat ccagacagtc aaatcccgtg ggggtaccgg tcacaccacc ggagccgagc
      121 acactttcca gttgctgcag aacggtatcg agcgcggatg aaagagaggc ttccgtgcgc
      181 tcccggagcg tcctgttctg cgcaagcgca aacagacgcc gctctgcagc atccagcaca
      241 tcccgtgacg tggcatttgg tgccgccaca tcggcaatca gggtgtttcc ggtggcctgt
      301 aactgacgca gaacgctttt ctccaccaca atgcctgcat aggccatgat gttggccgca
      361 gaaggtgtgt tcttgctgag ctcggccaga taagcaaaac cacccagtac gtccagcctg
      421 ccctgatgtt ccagatgctc tgacagtgta atcaggtcca gaggctggcc attcaccgcc
      481 agttcagaca tggcacggaa gatggcccgg tgcggagcca cataaaaatc gtcaggactc
      541 agcatcagca ccacttcatc ccagcgttca ttctccagca tcagcccacc cagaacggcc
      601 tgctctgctt cgtatgcaca gagaacagac atttcagcca tggcgtaccc tccctctctg
      661 actgccggaa acctggctac gtgaaaaaag gaaaaagggt gccggacgta cagttgtcgg
      721 gaataaaatc ccggtaactg tctgtacgtt ttcagaccgg tgagtttgcc
//
LOCUS       DAZKFU020000648          770 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZKFU020000648 DAZKFU020000000
VERSION     DAZKFU020000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.658"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..708
                     /gene="ydeO"
                     /locus_tag="ACHOQQ_RS26270"
                     /old_locus_tag="pgaptmp_005254"
     CDS             <1..708
                     /gene="ydeO"
                     /locus_tag="ACHOQQ_RS26270"
                     /old_locus_tag="pgaptmp_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HGU4473355.1"
                     /translation="LDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYL
                     QCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAA
                     CKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTF
                     SQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRC
                     QRHTGMNTDNTMSALAI"
BASE COUNT          241 a          154 c          156 g          219 t
ORIGIN      
        1 ttagataaag attacgcctt ctctgacggc gagatcctga tggtagataa cgctgttcgt
       61 acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga aaataccatt
      121 aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc tgcgaagttt
      181 ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta tctcaactcg
      241 gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct gtctattttt
      301 gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt ttccgggaaa
      361 gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga tatttgtgac
      421 tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca aacgacattc
      481 tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg cgtagaaggt
      541 tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt tatttatgcg
      601 ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg ttgtcaaagg
      661 cacaccggta tgaacacgga caacacgatg agtgctttag ctatttgatt atttgctaac
      721 gggtagtcaa ccacacacgc tacgtaagaa ttaaatgggg cagccattcc
//
LOCUS       DAZKFU020000649          768 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZKFU020000649 DAZKFU020000000
VERSION     DAZKFU020000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.659"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..557)
                     /locus_tag="ACHOQQ_RS26275"
                     /old_locus_tag="pgaptmp_005255"
     CDS             complement(<1..557)
                     /locus_tag="ACHOQQ_RS26275"
                     /old_locus_tag="pgaptmp_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HGU4473356.1"
                     /translation="MKKRFILSGLTVCLLMAGCQSTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGR"
BASE COUNT          193 a          210 c          215 g          150 t
ORIGIN      
        1 cgacccacaa aaggaacggt gaccggaaca ggagccagct ggctgaccgg taactggtaa
       61 ccgtcacgca gactgtggca gaccaccctc tgcacatcat ccacttccag ttgccatgcc
      121 ggccccgcca ttaactgcag ggcagtacgc agtctcagtg gtcccggctg atactgcacc
      181 gccggcagag gctgacggta cagcacgccg ttggccggtc cggtggcgca gagcgtgtaa
      241 ccggactgtt tcagggcata acgcatggca tcggccaccg tgggtttcag ggaggaggga
      301 atgcgaatgt cgatcacctg agaaagtgga tcacgctgta cagcctgcgg ctctgtgctg
      361 accagcaggt aacggtcata gcgcaccacc tccggtgcct gagaatacac atccgggctg
      421 gcaggctgta cattacggtt tactgtgacc gacggtaccg gcatggccgc atcccgttgc
      481 tgtagtttct gagtgctctg acatcccgcc atcaggaggc agactgtcag tccggacagg
      541 ataaagcgtt ttttcatcgg gtatttacct ctgcagtgat aaatcagagg tcaatgtgca
      601 gaagaagacg gggctggctc aatgaacaac tcattacccg ctggcgaaaa aaaacgcctg
      661 cagacatgca ggcgctttgt tgacaggaaa gaaagcaaca taacaaatat gtcacaatcc
      721 tgcggttaac ataaattacc gattaccgga gagcattacg caaatcat
//
LOCUS       DAZKFU020000650          767 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZKFU020000650 DAZKFU020000000
VERSION     DAZKFU020000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.660"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            216..291
                     /locus_tag="ACHOQQ_RS26280"
                     /old_locus_tag="pgaptmp_005256"
     tRNA            216..291
                     /locus_tag="ACHOQQ_RS26280"
                     /old_locus_tag="pgaptmp_005256"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Ile,seq:cat)
BASE COUNT          223 a          141 c          177 g          226 t
ORIGIN      
        1 gtatcattga atcgatgata actatagata gtgttaatta ttaacatttg cttgttgact
       61 gcgagtatcg actttgcgaa tggaggcgat gtcgggtttt atgtcgcatg tattgacgac
      121 ccaggatgga tgagaacgca taaaaatctg ttatttacat cataagatca tcatgtttat
      181 tgcgtggttt ataagtattg ttattgcata cttaaggccc tttagctcag tggttagagc
      241 aggcgactca taatcgcttg gtcgctggtt caaatccagc aggggccacc aaatcatcat
      301 tcatgtatca ggaagagtta agggtacaag gggtactcga tggcggtcta tatttcatga
      361 ctaacgccat ttaaaggaag aagtgcggtg ggtggtttcg aatgagggct ggtgttttgg
      421 tacgtggcac gccactgcaa tgtatttatc catcagtact cattcaaaag tacctggaaa
      481 aaatgcccgc tatgccaagc gggcaaattg cttacatgtg gaattaagta attaacgata
      541 aagccagagt attatcattt attataccca ttcatttaat caggttaatt actcaaatca
      601 ctgaactatt ctctgttcgc tcactgatga tgagtgagta ccacaaccaa tgtatgtaga
      661 acaatgccgt agagtaagga tgaaatgtct ctgccacgca ttaaacatct actactgacc
      721 agaacacccg ccgcaggtag atgagttttg tgagcgttac aggagcg
//
LOCUS       DAZKFU020000651          767 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZKFU020000651 DAZKFU020000000
VERSION     DAZKFU020000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.661"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..158)
                     /gene="ssb"
                     /locus_tag="ACHOQQ_RS26285"
                     /old_locus_tag="pgaptmp_005257"
     CDS             complement(<1..158)
                     /gene="ssb"
                     /locus_tag="ACHOQQ_RS26285"
                     /old_locus_tag="pgaptmp_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4473357.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKE"
     gene            complement(174..377)
                     /locus_tag="ACHOQQ_RS26290"
                     /old_locus_tag="pgaptmp_005258"
     CDS             complement(174..377)
                     /locus_tag="ACHOQQ_RS26290"
                     /old_locus_tag="pgaptmp_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473358.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(468..>767)
                     /locus_tag="ACHOQQ_RS26295"
                     /old_locus_tag="pgaptmp_005259"
     CDS             complement(468..>767)
                     /locus_tag="ACHOQQ_RS26295"
                     /old_locus_tag="pgaptmp_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HGU4473359.1"
                     /translation="RFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTL
                     NRGEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT          181 a          205 c          172 g          209 t
ORIGIN      
        1 tgttccttca tttcaccgct ctgtttatca cgccaggtat cagtggtggc cagcgtcaga
       61 tttgccactg cacctccgcc gttggggaaa taacggattt ccggatcctg tcccagatga
      121 ccaatcagta tgactttatt cactccacgc gatgccataa caactcctta tcctcacaca
      181 cattcacgct gagtccagat gccgttttcc agagcccgga ctgcctgctc ataatttctg
      241 aaatactctt cggattcccg ggacacaggc ccttcgtcgc actgagtacc aatataataa
      301 ccacgagcac tgctgaggat ctgcagaggt aaggtatagc ccgctttggc ggccagaatt
      361 ccgcttgtcg gtttcataac atgtttcctt aaagttaaac gataagaaaa cagacctccc
      421 tgatggttgg tctgttttca tcagggaggt tgagttgaaa aatacgatca gaaagaattt
      481 tctgcgtatt cccgcggggt attgccattc tgtggcggta tggattccga tttttcagtc
      541 ttataaacca tgtcctggcc tattttgatc cactccacct tgatcaggcg ggctttcaga
      601 ctgacgcgtt gctcaccggc atgttcaccc cggttaaggg taaaaatgtc agtagagggg
      661 ttgctcatgg taaaaccaat cagcaccttg cgatcttcat ccaccgcttt ctggcagcgg
      721 gcaatcaggg aagttgcctc tttaccggca accgtcacat caaaacg
//
LOCUS       DAZKFU020000652          750 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZKFU020000652 DAZKFU020000000
VERSION     DAZKFU020000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.662"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..656
                     /locus_tag="ACHOQQ_RS26300"
                     /old_locus_tag="pgaptmp_005260"
     CDS             <1..656
                     /locus_tag="ACHOQQ_RS26300"
                     /old_locus_tag="pgaptmp_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4473360.1"
                     /translation="LISANTYADQKETAINNRTDGLLMTERQARTAGDAATLAVARTH
                     SDENNKRTLISANTYADQKETAINNRTDGLLTTERQARIAGDAATLATAAAYSDAGDS
                     LTLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAGVTAISSIPY
                     VAENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGVAGGW"
BASE COUNT          235 a          204 c          172 g          139 t
ORIGIN      
        1 cactgatcag tgcgaatacc tatgctgacc agaaagaaac cgccatcaat aaccgtactg
       61 acggattact gatgaccgaa cgccaggcac gcaccgcggg tgatgctgcg acgctggcag
      121 ttgccagaac gcacagcgat gaaaacaaca aaagaacact aatcagtgcg aatacctatg
      181 ctgaccagaa agaaaccgcc atcaataacc gtactgacgg attactgacg accgaacgcc
      241 aggcacgcat cgcaggtgat gccgcaactc tggcaacagc agcggcatac agtgacgccg
      301 gggattcact gacactgaaa caggcaacag cctacagcga tatcagtacc cgccgggcac
      361 tgtcagaggc caatcgttat accgacaaca aattcagaaa cctgagcaat aaaattgaac
      421 gggcagaaaa aagacttaat gcaggcatcg ccggagtaac agcaatatcc tccatcccct
      481 atgtggctga aaattctttt tcatacggga tcggcgtggg taactatcag aacgctaatg
      541 ctgtggccgc aggcattcag tataaaacat cacgtaatac caatatacgc ctcaacatct
      601 cctgggactc atctcataac accgcgattg gtgcaggggt tgccggcggc tggtaaataa
      661 tcttgcgtac atacggcatt atggtattcc ataatcacat taattcagta cgcaacacag
      721 ccccatccgg aacagaccac tggactgagc
//
LOCUS       DAZKFU020000653          743 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZKFU020000653 DAZKFU020000000
VERSION     DAZKFU020000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.663"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..278)
                     /locus_tag="ACHOQQ_RS26305"
                     /old_locus_tag="pgaptmp_005261"
     CDS             complement(<1..278)
                     /locus_tag="ACHOQQ_RS26305"
                     /old_locus_tag="pgaptmp_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4473361.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERL"
     gene            complement(336..683)
                     /locus_tag="ACHOQQ_RS26310"
                     /old_locus_tag="pgaptmp_005262"
     CDS             complement(336..683)
                     /locus_tag="ACHOQQ_RS26310"
                     /old_locus_tag="pgaptmp_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4473362.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
BASE COUNT          162 a          211 c          182 g          188 t
ORIGIN      
        1 aggcgctcca ccgcctgctg gtaattaaat tcgtgtttcg gcttgacgta gcccggacgc
       61 aacacgcggg tttcaccacc acgatgacgc agcacttttc cttcaacaac cggggagaca
      121 taggctgcca ccggcgtttt tccggtaatt ttgtccagca tcacctcttc ggtgtggaaa
      181 ttcaccgtac ggcggaaaaa cagttccaga aacagcgcac gaaatttcac tttttgttcg
      241 gtataaccga gtaactggcg ggtcgtaaac aatcccataa atcagttcct ttcattcaga
      301 aatcagtcag gccaacgcgg tggcctgata acgtgttacg gcagcgccgc gtgactcagg
      361 gctgtgccga caaaggcgtt ggcctttttg tgttcatcca cactttcagg ccagcggatt
      421 gcctccgtcg caaaggtccc cgacttgtaa tacgtcagca ccgtctctgt gccttcaagc
      481 ggcagtacca gtatgccagc cgcactaccg gctttctgtc cgtcccagac caccagtttc
      541 ccgctggctt catccagcat caggggcgtc agtgccggtg ttgcctgaga aatcccgctg
      601 ctgcctgtgg cggtatgagc cggatcatta ccggcaaaaa tacgtacttc tgcacgttgt
      661 tcagtgatgg ttttcgttac catattgtaa aaacctcata ttgatggtca gcactgactt
      721 catggcatgg ccatgagcat ttt
//
LOCUS       DAZKFU020000654          737 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZKFU020000654 DAZKFU020000000
VERSION     DAZKFU020000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.664"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..297)
                     /locus_tag="ACHOQQ_RS26315"
                     /old_locus_tag="pgaptmp_005263"
     CDS             complement(<1..297)
                     /locus_tag="ACHOQQ_RS26315"
                     /old_locus_tag="pgaptmp_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HGU4473363.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIY"
     gene            complement(294..>737)
                     /locus_tag="ACHOQQ_RS26320"
                     /old_locus_tag="pgaptmp_005264"
     CDS             complement(294..>737)
                     /locus_tag="ACHOQQ_RS26320"
                     /old_locus_tag="pgaptmp_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4473364.1"
                     /translation="LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRK
                     TECYKLVRYLLSQSLIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARA
                     VLEIIEGNSDMTTIAHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          231 a          156 c          125 g          225 t
ORIGIN      
        1 ataaatagct ttgccatcat cataccaaat aagccccatc tgtgccgaca ggcgttcaat
       61 caaggcatag ggttctgtta aatcaaaatt tccgctgatc tgtttcttca tggcaagctt
      121 actaactatt gtaggcttac ccgcatatga tgaaaccgtt tcaaaaaaac tacgtagatt
      181 ttctttattt gctacgtatc cattcgctgc tgcggcatat gcaccaggag tcaataatcc
      241 attaaaaatg cataagactg aaattaatat aatagtaatg cgtaatttta ttttcataac
      301 tttttacata gttctctcgg tgtttttcct aaccggcttt ttacttcggc tgaaaagtgt
      361 gatgaggacg agtaaccata tttatgcgca atagttgtca tatcactgtt tccttcaatg
      421 atttccaaca cggctctggc aactctccag ccacataatt ccgttttaac tttaccaccc
      481 aatgcataac tacataaacg gcgaaaatgt gaatacgata cgccgtaaag ctcaccaaga
      541 tcatagagtg aagtctgtat gagcgattgc gataataaat aacgaaccag tttatagcat
      601 tctgtttttc ttagatgctg aaacaggatt tcattaatat tatcgctgct cagataagtg
      661 gctaaaaacc aggcttcact tgcaacttta ctgaatagca caacttctga tgcaaagggc
      721 aaggcatcag caaacaa
//
LOCUS       DAZKFU020000655          736 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZKFU020000655 DAZKFU020000000
VERSION     DAZKFU020000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.665"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(72..635)
                     /gene="ipbA"
                     /locus_tag="ACHOQQ_RS26325"
                     /old_locus_tag="pgaptmp_005265"
                     /gene_synonym="fimX"
     CDS             complement(72..635)
                     /gene="ipbA"
                     /locus_tag="ACHOQQ_RS26325"
                     /old_locus_tag="pgaptmp_005265"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HGU4473365.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          223 a          151 c          150 g          212 t
ORIGIN      
        1 atgggttgtt tttttatgga agaattgagg aaaacgtagg aatctgacat tttgaatcag
       61 aaggtactgt tttaacgagg cttttttttc cacacccctt taaatcgagc agcattactg
      121 gctgtgtatc taacagtatg ctggatattt cgatgaccca gataatcctg aagtagtcgg
      181 gtatcgacac cattgtcagc cagagcaaaa ccacaggcat gacgtaacat atgaggatgt
      241 gcttttacag ctaatcctgc acgttttcca gcctcacgaa tgatagaaaa aaaatgttgt
      301 ctgctgatag ggcggcgttt ccgggagaga aaaagccagt ctccttcaac tccgttttca
      361 attaacttcc tctgcttaag ccacaacttg atcaaattat attcatcagg aagaagagga
      421 tgtgtcgtcg agaaaccatt tttaatacgc cgaatattaa gttgttttcc tgatgcatca
      481 atatcggata aacgtaaatc aagaagttca ctggccctaa aaccgtgaat gaaagccatc
      541 ataattagac agcgatttct ttcaggaaag gacatgctct gggctgcatc catcagcctg
      601 tagacttcat cctgtgtgag atattttctc gtcatacgcc accgtgtaca gttgtaaaaa
      661 agagaggcac acccagataa gtatgcctgc catcagaacg ttatcgatgt aatctcatta
      721 tctcattaat aagatt
//
LOCUS       DAZKFU020000656          731 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZKFU020000656 DAZKFU020000000
VERSION     DAZKFU020000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.666"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..704
                     /locus_tag="ACHOQQ_RS26330"
                     /old_locus_tag="pgaptmp_005266"
     CDS             <1..704
                     /locus_tag="ACHOQQ_RS26330"
                     /old_locus_tag="pgaptmp_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4473366.1"
                     /translation="ANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELNTSLKF
                     VIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSNVNRTI
                     ANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRDVTEEE
                     GNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGLVDALE
                     DDDDVQKVYHNVANL"
BASE COUNT          244 a          127 c          155 g          205 t
ORIGIN      
        1 gggccaatat tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa atttatgcaa
       61 aattcggtgt agaaatctat gctgctgcta aacaaggtga acccgatcca gaattaaaca
      121 catctttaaa attcgttatt gaacgtgcaa aacaggcaca agttccaaag cacgttattg
      181 ataaagcaat tgataaagcc aaaggcggcg gagatgaaac gttcgtgcag ggacgttatg
      241 aaggctttgg tcctaatggc tcaatgatta tcgccgagac attgacttca aatgttaacc
      301 gtacgattgc taacgttcgc acaattttca ataaaaaagg cggcaatatc ggagcggcag
      361 gttctgtcag ctatatgttt gacaatacgg gtgtgattgt atttaaagga acagaccctg
      421 accatatttt tgaaatttta cttgaagctg aagttgatgt tcgtgatgtg actgaagaag
      481 aaggtaacat tgttatttat actgaaccta ctgaccttca taaaggaatc gcggctctaa
      541 aagcagctgg aatcactgag ttctcaacaa cagaattaga aatgattgct caatcagaag
      601 ttgagctttc cccagaagat ttagaaatct ttgaagggct tgttgatgcc cttgaagatg
      661 acgacgatgt acaaaaagtt tatcataacg tcgcaaatct ctaattatat tttaaagaaa
      721 tctgtcttta t
//
LOCUS       DAZKFU020000657          722 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZKFU020000657 DAZKFU020000000
VERSION     DAZKFU020000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.667"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>722)
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS26335"
                     /old_locus_tag="pgaptmp_005267"
     CDS             complement(<1..>722)
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS26335"
                     /old_locus_tag="pgaptmp_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4473367.1"
                     /translation="VRYREEWVTLQDAFGSEEEPIAVARFNFTIRFAHENNDSWKVCP
                     VARLIRDGQNAWRQDPSFIPPVALFGASPVLRERLVLLNRQLRSRRQRLMAMRRESNE
                     RLADFAVADVSLFWLLNALNSHASVLTEYERFPSRPPEQVWAELARLAGSMLTFSLDH
                     DPDAIPGYDHKDPAGAFLPLFDLITGLLEACLPSRVIALEMSRPDGQTWKASLHDIRL
                     REEADLWLSVRSDTPAWQVAEK"
BASE COUNT          186 a          209 c          193 g          134 t
ORIGIN      
        1 aatttctcag caacctgcca ggccggagta tccgagcgca cagacagcca gaggtccgcc
       61 tcttcgcgca ggcggatatc gtgaaggctg gccttccagg tctgtccatc cgggcgggac
      121 atttccagag caatgacccg ggatggcaga caggcctcca gcagacccgt tatcaggtcg
      181 aacagcggaa gaaacgcacc ggccggatcc ttgtggtcat aacccggtat ggcgtcagga
      241 tcatgatcca gggaaaaagt cagcatactg cctgccagac gagcgagctc cgcccacacc
      301 tgttcaggtg gacgggaagg aaaacgttcg tattccgtca gtacactggc gtgggagttc
      361 agcgcattca gcaaccagaa aagggaaaca tcagcgacag caaaatcggc gagtctttcg
      421 ttactttcac gacgcatcgc catcagacgc tggcgtcggg agcgtaactg acgattgagc
      481 aacaccagac gttcgcgcag aaccggactt gccccaaata acgccaccgg aggaataaaa
      541 gacggatcct gtcgccacgc attctgacca tctctgatca atcttgctac cggacagact
      601 ttccatgaat cattgttttc atgggcaaac cggatggtga aattaaaccg ggcaacggct
      661 atgggctctt cttcagagcc aaaagcatcc tgcagcgtga cccactcttc ccgatagcga
      721 ac
//
LOCUS       DAZKFU020000658          720 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZKFU020000658 DAZKFU020000000
VERSION     DAZKFU020000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.668"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..248)
                     /locus_tag="ACHOQQ_RS26340"
                     /old_locus_tag="pgaptmp_005268"
     CDS             complement(<1..248)
                     /locus_tag="ACHOQQ_RS26340"
                     /old_locus_tag="pgaptmp_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473368.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCSSFHRRVHERITERDHTVSPSAPVVLRWLREQ"
     gene            complement(529..707)
                     /locus_tag="ACHOQQ_RS26345"
                     /old_locus_tag="pgaptmp_005269"
                     /pseudo
     CDS             complement(529..707)
                     /locus_tag="ACHOQQ_RS26345"
                     /old_locus_tag="pgaptmp_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
BASE COUNT          181 a          180 c          183 g          176 t
ORIGIN      
        1 tgttgttccc gcaaccagcg taataccacc ggcgctgatg ggctgaccgt gtgatcacgc
       61 tccgtaatac gttcgtgcac ccgtcggtga aaggaactac agaatgtctg aatctgttga
      121 ccacagacgc cttctgtcga atgaagtgtg aagtggattg ccagcagatg cggccagtga
      181 tccaccgcct gctgaacaaa acgccggatt tcccccggct ctgaaagtaa ggcttcggtt
      241 atttgcacta ttttatctct gttgaacttg gttaagtcgg tgcagacgca tcaacacaag
      301 tacggttcga tgcaaacagc tgtgactggc aatatgaaag gaatgatgaa tcagtcagga
      361 tgacaaagtg ccagctgacc ggaggggacg caggaagatt cacgggggga ccagcaccag
      421 ggaacagcgc cacaatacca acgctgacac gttgaacatt gccagcgtac cggtatcaca
      481 acacgtttca tacttctgcc accgtgattc ttcgattcgt tactgtatct actgtgacac
      541 ttcgctttta tacctgcggc tggatcggcc cggcttgatg aatcttcact gatcagctta
      601 taaacccctc tgtcggtcat accggtgaaa ctggtgatat agttcatgtc aatcagggaa
      661 ttatcggcac gcagaaatac gctgtcgtgg cttgttgtag tcaacatggt cagaatgtcc
//
LOCUS       DAZKFU020000659          718 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZKFU020000659 DAZKFU020000000
VERSION     DAZKFU020000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.669"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            496..>718
                     /locus_tag="ACHOQQ_RS26350"
                     /old_locus_tag="pgaptmp_005270"
     CDS             496..>718
                     /locus_tag="ACHOQQ_RS26350"
                     /old_locus_tag="pgaptmp_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473369.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQE"
BASE COUNT          207 a          145 c          216 g          150 t
ORIGIN      
        1 ggggttaagg gataaaaaaa tcccgcagag tccggagact gcgggacaga accgatgaag
       61 attaacgttc aaggagtttt gtttttattg acccgaaaaa actgtaacgc cccggaatga
      121 tatctgccac gggaacgtac agaaaatgtg aagaaattca gactttttct gtcgcaggtt
      181 tacagaacgg atgaccgcca gggtaccagt ggcgtaaatg agcaggatgt tgtggtgtgg
      241 ctgaagcaaa aaaatctccc gtaatatcgc cggatactga cgggagacga atagtcacct
      301 taaggaggtc tgataattat tgcttttatt gagcaaaaaa actgtaacgc tgcgtcatta
      361 tagtggctac aggcaattgc cggaaatgtg aagaatttca gaaattttat tccgtcatga
      421 cacaggcacc ctccggggtg cctgtcgttt tctggcataa acagattcag acatcagaca
      481 ggagaggggg acagagtggg taaagggggc ggcaaggggc acacaccgcg tgaggcgaag
      541 gacaatctca aatccacgca gatgatgagc gtgattgatg ccattggtga gggaccggtg
      601 gaaggtccgg tgaagggact gcagagtatt ctggtgaaca aaaccccgct gacggacacg
      661 gacggtaatc ccgtgataca cggtgtgacc gccgtctggc gtgccgggga gcaggagc
//
LOCUS       DAZKFU020000660          718 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZKFU020000660 DAZKFU020000000
VERSION     DAZKFU020000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.670"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..540
                     /locus_tag="ACHOQQ_RS26355"
                     /old_locus_tag="pgaptmp_005271"
     CDS             22..540
                     /locus_tag="ACHOQQ_RS26355"
                     /old_locus_tag="pgaptmp_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HGU4473370.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            537..>718
                     /locus_tag="ACHOQQ_RS26360"
                     /old_locus_tag="pgaptmp_005272"
     CDS             537..>718
                     /locus_tag="ACHOQQ_RS26360"
                     /old_locus_tag="pgaptmp_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4473371.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVM"
BASE COUNT          144 a          190 c          227 g          157 t
ORIGIN      
        1 tgaaccacag gaggagcaga aatgaaacga gaaagacagt tttatgtact gagtctgctg
       61 ctgttctgta caacagctgg cgcagaactg aaagtgattg cagacctggg cggcagggat
      121 gccagcccct tttttgaggc gataaaccgg cagccaccgg ttcccgcttc agttgtgcca
      181 tcaccctctt ttccgcaggg agaggcggca atgttaccgg tcagtacgcc tgaaatgtca
      241 cccggagagg ttgtggcgcg ccggcttcag ttaccgggca tcggtgcact ttttcttgtg
      301 ggcgatgatg cccattcccg cacatggctg cagatgaatg cagcagtact cagggagcgg
      361 aaggcggcgg gaatggtagt gaatgtggcg gatgagagca gtctgcgcgt actgcgtgag
      421 ctggcaccgg gggtggtgat ggttccggca tccggttctg tactggcccg caggttacag
      481 ataagtcatt acccggtgct gataacggac aacggcctgt cacaggtacc tgcaccatga
      541 tgccgctgtc tgccacttct gctgcgctct ctgcttctcc ggtactgaca gcctgcctgg
      601 ttctcagcct gctggctgtt gtcctggggc cggcactgtg gcgcagccgg cgaaaaaaag
      661 cgtgtgtgag aacacgccgg catcagcgtt atcgtgccac ggcctcacgg gtaatgaa
//
LOCUS       DAZKFU020000661          713 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZKFU020000661 DAZKFU020000000
VERSION     DAZKFU020000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.671"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..641)
                     /locus_tag="ACHOQQ_RS26365"
                     /old_locus_tag="pgaptmp_005273"
     CDS             complement(<1..641)
                     /locus_tag="ACHOQQ_RS26365"
                     /old_locus_tag="pgaptmp_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4473372.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRT"
BASE COUNT          240 a          181 c          126 g          166 t
ORIGIN      
        1 attgttctga cgggtttatt ctcttgttgc cttttctgcc agtcatcaga ctccggaaga
       61 ttcttgcgaa gataaaaagc aaataatatt ggaagcatgc cgacaaagaa aagagaacgc
      121 cagccccata gtggtactac aatgctataa acctgtgcag ctaaacctcc cccaatagaa
      181 aatccagaaa ttaaaaaacc actcgccttg tttctgagat gaacaggcca gctttcgata
      241 acatatgtta cacttgagcc atattcacct gccataccaa gcccaataac aatgcgagca
      301 ataaacatca ctgcatatcc aggagccaga ccacaggcaa gggttccgag agagaatagc
      361 actatactta aaaccattgc catcctgcgc cccatcttgt ccgataaagc gccaatagca
      421 agccccccaa accatcggga aataaaagca gcggaaataa ggcttgccgc ttctattgtc
      481 gtcaagccga attcatgctg aacctctgta agaaccagag atatcagaac gaaatcaaaa
      541 ccatcaagta aataacctgt ccatgccgca ccgaatatct tccactgtct gggtgaaaga
      601 tgcttatacc aaacctgctt tttctgaata caggagtcca tattaaacct cacaataata
      661 gcggatttat aatacactgg agtattactt taagtaatac aatcccggtc cca
//
LOCUS       DAZKFU020000662          710 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZKFU020000662 DAZKFU020000000
VERSION     DAZKFU020000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.672"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          236 a          132 c          139 g          203 t
ORIGIN      
        1 agccgtcaat tgggacagtt ttttataact cattaatccc tacctgttgc tttattttca
       61 ttatgaaaag ttaccaaatc ttagcacctc agtcaggaga gtcaggtcat cgataaagaa
      121 tattccagta gagtggcgct catgatcaaa gataacagca aagttgatga cagcgctgac
      181 cgactgtcat ttacagaaac cctgtttgta gcacattcca tcttacctga cctcacccag
      241 acaaagacaa aagagaatga ttgttattta cttttcaaaa aaaagtgtca tattgtccat
      301 ataaagtcaa gaggtatttt acgttatgtc actgatgcaa ttgttagtca ggatctcatg
      361 gttgtcgctc atgtataacc tgagacttga acgcacacta atagcactga cggtcataca
      421 tcgggaaatg tgaggaggca taagaatgaa ttgtgataat aatcacagaa atgaagaatt
      481 cattgttacc tttgataaag gcaacaagca agacaattca agacgaaaac acgataattt
      541 tcctatagag gtagaatcct ccgtagagca ggagacacac tgtatcacaa ataataagtc
      601 ggcttccggt atagtaacac atgactatga tgccgattat atttgtggtt gtggtgaaat
      661 tatgtgtcct ggttgcggtc atgacctata acaaacattt ttgcaaactg
//
LOCUS       DAZKFU020000663          709 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZKFU020000663 DAZKFU020000000
VERSION     DAZKFU020000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.673"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..634)
                     /gene="queE"
                     /locus_tag="ACHOQQ_RS26370"
                     /old_locus_tag="pgaptmp_005274"
     CDS             complement(<1..634)
                     /gene="queE"
                     /locus_tag="ACHOQQ_RS26370"
                     /old_locus_tag="pgaptmp_005274"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGU4473373.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWR"
BASE COUNT          169 a          198 c          161 g          181 t
ORIGIN      
        1 aacgccaatt acgcgcaatg caggtttcaa tgcacaaacg tgtggcatca tccttttggc
       61 taatcggctg cagtgcaatg actcgcggtt tatcatcggt cagcgtcgcc aaaagttcat
      121 ccagtgcttc aatatcacgt acgcgtccca ctggatgctt gatttcgttg gctcgctcca
      181 gtgcctgtga caacacttca tagccgccgc gcatgttcag ctttggcgat acggtaaccc
      241 aggtattcgg tgtacagcgt acttcatgag taccactggt ttcaatctgg cagctaaaac
      301 cgttcttttc gagcaggtca gtcagtggca gcaaatcatg aatgcaaggc tcaccacccg
      361 taatcaccac atgccgcgcg gtatatccct ggcgaccaat gacagccagc aagtcttcac
      421 tgctcgccgc cccccactta tcactctctt tggtcttcgc cagaatgctg aaaagggaga
      481 cttcccgatc ctcaagcttt tcccaggtgt gtttggtgtc gcaccaggca cagccaaccg
      541 ggcatccctg taaacgaata aaaatggcgg ggacgccggt aaagtaaccc tcaccttgca
      601 gggtctggaa catctcgtta atcgggtact gcatagcatt ctctgtgaag tggataattg
      661 ttaattattg cagatcctgc cacaacaatc atgtcttatt aacattctg
//
LOCUS       DAZKFU020000664          704 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZKFU020000664 DAZKFU020000000
VERSION     DAZKFU020000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.674"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(246..>704)
                     /locus_tag="ACHOQQ_RS26375"
                     /old_locus_tag="pgaptmp_005275"
     CDS             complement(246..>704)
                     /locus_tag="ACHOQQ_RS26375"
                     /old_locus_tag="pgaptmp_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4473374.1"
                     /translation="QNLSLQKTLPQQFSEQMFFACLSIFSTHRGFMLLLFRCINSVAL
                     KVRYIICSSPSRAWRVKDISNSLYMSESLLKRKLQEEKVSFSQILLDIRMHNARQLIS
                     SKLSVNQIAIRCGYASTPYFISVFREYFGITPHQMMINQSGFIESRIAIK"
BASE COUNT          215 a          140 c          142 g          207 t
ORIGIN      
        1 caggaaacgg attgcaggag aggtatactc attcatcttc ccggagtttt gttaatcact
       61 gcataatcaa acggaccaat acattctttg ctcgcatccg taaaaatgtc gccacaatat
      121 catcagtcag tcaactcatt accatctgtt ttccgggtcc gtagttacta cagtgctccg
      181 gagtgataaa cagaaactga taatgaagca taagaatact tttattatgc gctgactgac
      241 atactttact taattgcgat tctgctctca ataaatccgg actgattaat catcatctga
      301 tgaggtgtta taccaaaata ttctctgaaa acgctgataa aatacggtgt gctggcataa
      361 ccgcaacgta ttgcgatttg attcactgac agtttactgg aaataagttg tcgggcgtta
      421 tgcatgcgta tatccagcag gatttgggag aaagaaactt tttcctcctg aagttttctt
      481 ttcagtaaac tctcgctcat gtacagagaa ttactgatat ctttaactct ccatgcccgt
      541 gatggactgg aacagataat atacctgact ttaagcgcaa cagagtttat gcatctgaag
      601 agcagaagca tgaaacctct gtgtgtggaa aaaatggaaa ggcaggcaaa aaacatctgc
      661 tcagaaaatt gttgtggcaa tgtcttttgc agactgagat tctg
//
LOCUS       DAZKFU020000665          693 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZKFU020000665 DAZKFU020000000
VERSION     DAZKFU020000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.675"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..686
                     /locus_tag="ACHOQQ_RS26380"
                     /old_locus_tag="pgaptmp_005276"
     CDS             105..686
                     /locus_tag="ACHOQQ_RS26380"
                     /old_locus_tag="pgaptmp_005276"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4473375.1"
                     /translation="MNHLMIDTETLGNGPDAAIFAIGAVFFDPFTGKLGKQFEKFIDP
                     VDSERNGGTVSATTAVWWAGQSVEARACLRNAEGTELAAVTEFLAFISRNMHDESPGN
                     SLTIWCKGASFDFPILRSAIKRTTGEKSIPWCYRDERCMRPLIAMAESTGWRMPGRSG
                     KEAIAHTALSDAVYQAKVVSEIWQRFTTPFLNI"
BASE COUNT          191 a          188 c          190 g          124 t
ORIGIN      
        1 gagacagcga aagagacaca cacctgtcca caggaaacct gcgcagccat tgaacgactg
       61 attgcaatac tcgccaaccc agacgaactg aaggggtgcg cataatgaac catctcatga
      121 ttgacaccga aacactgggt aacggaccgg acgcagccat attcgcgatc ggggcggtgt
      181 ttttcgaccc gttcaccgga aagctgggca agcagtttga gaaattcatc gacccggtag
      241 attcagaacg caacggcgga accgtcagtg caacaaccgc tgtatggtgg gccggacaat
      301 ccgtggaggc cagggcctgc ctgcgcaacg cagagggaac agagcttgca gcagtaaccg
      361 aatttctggc gttcatttca cgcaatatgc atgacgaaag cccgggcaat tcactgacca
      421 tatggtgcaa gggcgcatcc ttcgatttcc caatcctgag atccgccata aagcgaacaa
      481 ccggagaaaa aagtattccg tggtgttacc gggacgagcg ctgtatgcgt ccgcttatcg
      541 ccatggctga atcaaccggc tggagaatgc caggccgatc aggaaaagag gccattgctc
      601 acaccgcgct aagcgacgcc gtttaccagg caaaggtcgt gtcagaaatc tggcagcgct
      661 tcacaacgcc attcctgaac atatgaggtg aag
//
LOCUS       DAZKFU020000666          691 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZKFU020000666 DAZKFU020000000
VERSION     DAZKFU020000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.676"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            104..685
                     /locus_tag="ACHOQQ_RS26385"
                     /old_locus_tag="pgaptmp_005277"
     CDS             104..685
                     /locus_tag="ACHOQQ_RS26385"
                     /old_locus_tag="pgaptmp_005277"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4473376.1"
                     /translation="MNHLMIDTETLGNGPDAVIFAIGAVFFDPFTGKLGKQFEKFIDP
                     VDSERNGGTVSATTAVWWAGQSVEARACLRNAEGTELAAVTEFLAFISRNMHDESPGN
                     SLTIWCKGASFDFPILRSAIKRTTGEKSIPWCYWDERCMRPLIAMAESTGWRMPGRSG
                     KEAIAHTALGDAIYQAKVVSEIWQRFTTPFLNI"
BASE COUNT          189 a          185 c          188 g          129 t
ORIGIN      
        1 gagacagcga aagaaaaaca cacctccccg ctggaacctg ttcagtcatt gaacgactga
       61 ttgcaatgct caccagccag gacgaaccga aggagcccgt acaatgaacc atctcatgat
      121 tgacaccgaa acactgggta acggaccgga cgcagtcata ttcgcgatcg gcgcggtgtt
      181 tttcgacccg ttcaccggaa aactgggcaa gcagtttgag aaattcatcg acccggtgga
      241 ttcagaacgc aacggcggaa ccgtcagtgc aacaaccgct gtatggtggg ccggacaatc
      301 cgtggaggcc agggcctgcc tgcgcaacgc agagggaaca gagctggcag cagtgaccga
      361 atttctggcg ttcatttcac gcaatatgca tgacgaaagc ccgggcaatt cactgaccat
      421 atggtgcaag ggcgcatcct tcgatttccc aatcctgaga tccgccataa agcgaacaac
      481 cggagaaaaa agtattccgt ggtgttactg ggacgagcgc tgtatgcgtc cgcttatcgc
      541 catggctgaa tcaaccggct ggagaatgcc aggccgatca ggaaaagagg ctattgctca
      601 cactgcactg ggcgatgcca tttaccaggc aaaggtcgtg tcagaaatct ggcagcgctt
      661 cacaacgcca ttcctgaaca tatgaggtga a
//
LOCUS       DAZKFU020000667          687 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZKFU020000667 DAZKFU020000000
VERSION     DAZKFU020000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.677"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(96..473)
                     /locus_tag="ACHOQQ_RS26390"
                     /old_locus_tag="pgaptmp_005278"
     CDS             complement(96..473)
                     /locus_tag="ACHOQQ_RS26390"
                     /old_locus_tag="pgaptmp_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4473377.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            complement(478..>687)
                     /locus_tag="ACHOQQ_RS26395"
                     /old_locus_tag="pgaptmp_005279"
     CDS             complement(478..>687)
                     /locus_tag="ACHOQQ_RS26395"
                     /old_locus_tag="pgaptmp_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473378.1"
                     /translation="YTEGVVLAKRTEALFRVVPPSLYLALGMTEKEEKAERRALMKVH
                     QCSELEAAFHVARKLDGLRGLVGRE"
BASE COUNT          161 a          248 c          142 g          136 t
ORIGIN      
        1 ccacagctcc cgccagaaac agcacaggtt cactggcaat ttgatctgac agcgatggat
       61 acagtggtcc cggaaaatcg atatcgctcc tcatatcacc cctctcccag ccacaacagc
      121 agatagaaca aatccgccgc atcagtcact gatatatacc agcggggatg aatatactcc
      181 tcctccgacg gccagctctc atgcccactc acagtaagca gaatatgcac atactgcccc
      241 tgctgacctt ccagcatcac cgccacactg ctgttcagac ccaccctctg tggctggcaa
      301 cggatacact ccacccgccc cagttgcgcc gccagatgcc cgcgaagacg ttcaagatta
      361 tcccgtgtgg tgcaacttat ccccggcgca ccctgtggcg gcagaacaaa cacacttccg
      421 gcagggtgaa cagcagacac cacttccggt tccggatact tcagacacag catagtctca
      481 ctccctgcca accagaccac gtagcccgtc cagctttcgt gccacatgaa acgctgcttc
      541 cagttcgctg cactggtgca ccttcatcag ggcgcgtcgt tcagccttct cttctttctc
      601 cgtcatcccc agagccagat acagactcgg tggcacaacc cggaacaacg cttctgtgcg
      661 cttcgccagt accaccccct ccgtata
//
LOCUS       DAZKFU020000668          683 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZKFU020000668 DAZKFU020000000
VERSION     DAZKFU020000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.678"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..>683
                     /locus_tag="ACHOQQ_RS26400"
                     /old_locus_tag="pgaptmp_005280"
     CDS             43..>683
                     /locus_tag="ACHOQQ_RS26400"
                     /old_locus_tag="pgaptmp_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGU4473379.1"
                     /translation="METVGNFIQGKTRLSNSGETLPVTNPATGEVIRLVTQSTRQDML
                     EAIQSAHEAFPAWSKMAPLRRARILFEFKVLLEKHRDELAALIVSEHGKVWSDALGEL
                     TRGIEVVEFACGIPHLIKGEFSFNVGTGVDSFSLMQPLGVVAGITPFNFPAMVPMWMF
                     PIALACGNTFVLKPPALVPSASLRMAQLLQEAGLPDGVFNVVHCGNDAASLLT"
BASE COUNT          171 a          162 c          178 g          172 t
ORIGIN      
        1 agtcggaacg tcgttaacat tgattcaaga aggtaaacag tgatggaaac agttggaaat
       61 tttattcagg gtaaaacgag actgagcaac agtggtgaaa cactaccggt gactaaccct
      121 gcaaccggag aagtgattcg cctggttacc cagagcaccc ggcaagatat gctggaggcg
      181 attcagagtg ctcatgaagc ttttcctgca tggagtaaaa tggcaccgtt gcgccgtgca
      241 agaatccttt ttgaatttaa agtgttgctg gaaaaacacc gagatgaact ggcggctctg
      301 atcgtcagtg aacatggcaa agtatggtcc gacgcgcttg gtgaactgac tcgcggaatc
      361 gaagttgttg aattcgcctg cggcattccg catctcatta aaggagagtt ctcttttaat
      421 gttggcacag gcgttgacag cttttcatta atgcaaccac ttggtgttgt agctggcatc
      481 acacccttta acttccctgc aatggtgcca atgtggatgt tccctatcgc tctcgcctgc
      541 ggcaatacct tcgtcctgaa gcctccagcg ctggtgccct ccgcatcgtt acgtatggcg
      601 caactactac aggaggccgg actaccggat ggtgtattca atgtagttca ctgtggcaat
      661 gacgcagcca gcctgttgac cag
//
LOCUS       DAZKFU020000669          683 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZKFU020000669 DAZKFU020000000
VERSION     DAZKFU020000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.679"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            513..>683
                     /locus_tag="ACHOQQ_RS26405"
                     /old_locus_tag="pgaptmp_005281"
     CDS             513..>683
                     /locus_tag="ACHOQQ_RS26405"
                     /old_locus_tag="pgaptmp_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4473380.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPV"
BASE COUNT          165 a          169 c          180 g          169 t
ORIGIN      
        1 attgctggtt tcgtggtgtg cagcaatgta gttacagtgc aatcaatgtc acaattcagt
       61 cagttgacag ttgcctgtct ggctgagcat ttgttaaaaa aatcctgcat gatgaatccc
      121 cctgagcgga ggggcataat gacagatgtt tggttgcgta ttgtataggc aagttgcgga
      181 ttctgtctgg tcattgcaga attcaccggg aggcacccgg catcatgctg tatacagaga
      241 ttaggcatat atccaggctc ctcatcgcag gagccttttt acatgcaaaa aaagcccgcg
      301 ccgggagacg cgggcagcaa ggaataaaca acaaaacgtg aagtaatcaa tttttcagct
      361 ggcgaataat acccgacagt aatcactctg cgcaactgcg cggccttttt cgtattgcgg
      421 gctgtagtct tccttctgtc attgtcctgt aacttccgga cttcagcccg ccccttatct
      481 gactcacaac attatcccgg ccgggaggat tcatggcatt taaacactat gacgtggtca
      541 gggcggcgtc gccgtcagac cttgcggagc gactgactca aaaactgaag gaggggtggc
      601 agccatttgg cagcccggtg gccatcacgc cttataccct gatgcaggcc attgcggcgg
      661 aaggtgatgt caccacacct gtg
//
LOCUS       DAZKFU020000670          676 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZKFU020000670 DAZKFU020000000
VERSION     DAZKFU020000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.680"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            96..335
                     /locus_tag="ACHOQQ_RS26410"
                     /old_locus_tag="pgaptmp_005282"
     CDS             96..335
                     /locus_tag="ACHOQQ_RS26410"
                     /old_locus_tag="pgaptmp_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HGU4473381.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            355..>676
                     /gene="sctI"
                     /locus_tag="ACHOQQ_RS26415"
                     /old_locus_tag="pgaptmp_005283"
     CDS             355..>676
                     /gene="sctI"
                     /locus_tag="ACHOQQ_RS26415"
                     /old_locus_tag="pgaptmp_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HGU4473382.1"
                     /translation="MSVSNMPPIDRVEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETL"
BASE COUNT          227 a          113 c          149 g          187 t
ORIGIN      
        1 ggacacaaat aatagttcat ttagctacgg agataaccga ttcgagaagt ctcaaggtag
       61 caagtggaca tttcaggaat aattgaggag gacttatggc agattggaat ggttatatta
      121 tggatatcag caaacaattt gatcagggcg ttgatgatct gaaccagcaa gttgaaaaag
      181 cgttggagga tttagcaacc aatccttccg acccgaagtt ccttgccgaa tatcaaagtg
      241 cattagctga gtatacatta tatcgaaatg cgcaatctaa cgttgtaaaa gcgtataaag
      301 atctcgattc tgcaatcata caaaacttcc gctaaactgg ataaggattt tgttatgtca
      361 gtaagtaata tgccacctat tgatagagtt gagcaatcaa ctgctcatga aatacagcaa
      421 gcgaaagtga ttgatttaaa tgaccgggtt ctcaatcttg acaacccgga tgataaaatg
      481 atctccgctt ttgccaacta tgctgtacaa actgaaaatt ggcagcaaaa tgcgttacaa
      541 gcattaaggt ctgataaaaa ggggttaact cctgaaaaat tattagtctt gcaggatcat
      601 gtgcttaatt ataatgtcga ggtttcattg gtgggaacat tagcaagaaa gattgtcgct
      661 gcagttgaga cgttga
//
LOCUS       DAZKFU020000671          668 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZKFU020000671 DAZKFU020000000
VERSION     DAZKFU020000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.681"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..198)
                     /locus_tag="ACHOQQ_RS26420"
                     /old_locus_tag="pgaptmp_005284"
     CDS             complement(<1..198)
                     /locus_tag="ACHOQQ_RS26420"
                     /old_locus_tag="pgaptmp_005284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473383.1"
                     /translation="MFSFPASSCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQG"
     gene            311..>668
                     /locus_tag="ACHOQQ_RS26425"
                     /old_locus_tag="pgaptmp_005285"
     CDS             311..>668
                     /locus_tag="ACHOQQ_RS26425"
                     /old_locus_tag="pgaptmp_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473384.1"
                     /translation="MDEGKQPGTPGMNDIISIIILLTVYLIGLIFIILTINDIDRSSK
                     KRKYEKKLKEAIINGSLQNNDICLLAERWSASHEHIKSALLSVFNDFLTDNNFSHHVP
                     RVRELITWYEKYAPFTE"
BASE COUNT          209 a          163 c          154 g          142 t
ORIGIN      
        1 cccctgtgaa gaagcctgca gagaggccgt tctggcccgc tccagcgctg cctgcacgtg
       61 gtcaagctgg cgcattacag tggccagttc atccttttcg gcagcctgcg caggtaatgc
      121 aaaggacaaa agaaacagca tccccgcggc accggccaga cggccggcac ggcaggatga
      181 tgccggaaaa gaaaacatac gcacctccgt cagagttaaa cagtgcgtac aggatgcgta
      241 aggcagggga ctggatcagc gataaactct atttgcaaaa cagaaaattt ttacgggaga
      301 atacactggt gtggatgaag gcaaacaacc cggaactcct ggcatgaacg acatcatcag
      361 tataatcatt ctcctgacag tctatctcat cggcctgata ttcattatcc tgactatcaa
      421 tgatatagac agatcatcaa aaaagagaaa gtacgagaaa aaactgaaag aagcgataat
      481 aaatggcagt ttacaaaata atgatatctg tttactggcc gaacgctggt ctgccagtca
      541 tgaacatatc aaaagcgcac tgctgtctgt tttcaatgat tttctgactg ataacaactt
      601 ttcacatcac gtcccccggg tcagagaact gatcacatgg tacgagaaat atgccccatt
      661 cacagaac
//
LOCUS       DAZKFU020000672          665 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZKFU020000672 DAZKFU020000000
VERSION     DAZKFU020000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.682"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>665
                     /locus_tag="ACHOQQ_RS26430"
                     /old_locus_tag="pgaptmp_005286"
     CDS             <1..>665
                     /locus_tag="ACHOQQ_RS26430"
                     /old_locus_tag="pgaptmp_005286"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4473385.1"
                     /translation="NPRINRVWCVKEKKEHPQIGSSEWIVIRQPLIHEGFLAYFLQTQ
                     DFRDLLCSDVSGVGGSLTRAQPKRVATYNVPIAPLPEQKIIAEKLDTLLAQVESTKAR
                     LEQIPQILKRFRQAVLGAAVSGKLTQNWRENNKTTPINKLLNKINEERNEARKNKKIK
                     QNFGKYRPVLPLFQLPADCWEWVCFEQICETDNNALKAGPFGSALKKSDCVKSGHKVY
                     GQE"
BASE COUNT          227 a          124 c          138 g          176 t
ORIGIN      
        1 ttaatccccg tattaatcgc gtttggtgtg taaaagaaaa aaaagaacat cctcaaatcg
       61 gttcatctga atggattgta atcaggcaac cgctcattca tgaaggtttt ttggcctatt
      121 ttttgcaaac acaagatttc agagatctcc tatgttctga tgtttctggt gtgggtggtt
      181 ctctgacgag agcgcaaccc aaaagagtcg ctacatataa tgtccctata gcacctttgc
      241 cagaacaaaa aatcatcgct gaaaaactcg atacgctgct ggcgcaggta gagagcacca
      301 aagcacgtct tgagcaaatc ccacaaatcc tgaaacgttt tcgtcaggcg gtgctgggtg
      361 ctgctgtcag cggaaaactt acacaaaatt ggagagaaaa caataaaaca accccaatta
      421 acaaattatt aaataagata aatgaagaaa gaaatgaagc cagaaaaaac aaaaagataa
      481 aacaaaattt tggtaaatat agacccgtac ttccattatt tcaattgcct gccgattgtt
      541 gggagtgggt atgttttgaa cagatttgtg agactgataa caatgcgtta aaagctgggc
      601 catttggttc ggcactcaaa aaatctgact gtgttaaatc tggtcataaa gtttatggtc
      661 aagag
//
LOCUS       DAZKFU020000673          663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZKFU020000673 DAZKFU020000000
VERSION     DAZKFU020000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.683"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>663)
                     /locus_tag="ACHOQQ_RS26435"
                     /old_locus_tag="pgaptmp_005287"
     CDS             complement(<1..>663)
                     /locus_tag="ACHOQQ_RS26435"
                     /old_locus_tag="pgaptmp_005287"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HGU4473386.1"
                     /translation="AIIPTRQVFDLSSLNGDELKVYQLIRQHYLAQFLPEQEADVTEA
                     TFVIGGQLFRSRGRVNVIIGWKQLFPPENHADEEKPDSDESGNILPPLIKGELCALDG
                     AEQKELKTVPPRPFTEGSLITAMKNASSGVTDPALKKILRENAGLGTEATRAGILETL
                     FARNYIERAGKHIRSTQLGRELVAALPDSLTNPGMTALWEQALDDVARGKISLEAFME
                     KQ"
BASE COUNT          151 a          195 c          140 g          177 t
ORIGIN      
        1 tgctgtttct ccataaacgc ctccagtgag attttccccc gggcaacatc atccagcgcc
       61 tgttcccata aagccgtcat acccggattt gtcagagagt ccggcaatgc cgccaccagt
      121 tctcttccca gttgcgtgga acggatatgt tttcccgctc tctcaatata gttgcgcgca
      181 aataacgttt ccaggatccc cgcgcgtgtg gcttccgttc ccagtcccgc attctccctc
      241 aggatctttt tcagcgccgg atccgttaca ccagacgaag cattcttcat cgcagtaata
      301 agactgcctt cagtaaaagg acggggaggt acggttttca gctccttctg ttcagcacca
      361 tccagagcac agagttctcc ctttatcaaa gggggcaata tgtttccact ttcatcactg
      421 tcaggctttt cttcatctgc atgattttcc ggtggaaata actgtttcca cccgataatc
      481 acattcactc ttccgcggga acggaataac tgccccccaa tcacaaatgt tgcctcagta
      541 acatcggctt cctgctccgg cagaaactgt gccagatagt gctgacgaat cagttgatat
      601 accttcagtt catcaccgtt cagtgagctg aggtcaaaaa cctgccgggt cggaataatg
      661 gca
//
LOCUS       DAZKFU020000674          662 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZKFU020000674 DAZKFU020000000
VERSION     DAZKFU020000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.684"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>662
                     /locus_tag="ACHOQQ_RS26440"
                     /old_locus_tag="pgaptmp_005288"
     CDS             <1..>662
                     /locus_tag="ACHOQQ_RS26440"
                     /old_locus_tag="pgaptmp_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGU4473387.1"
                     /translation="RMEDKRQEEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTD
                     EHGAVRAAKGLFLTADAQARAQGPVLEMAAAISQINQANSQMQALNSAAEAAGALLSD
                     INTHIRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLCSTRNTMINAGQHLDIGAMK
                     NLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVTSSEDEIIIST
                     PET"
BASE COUNT          179 a          164 c          199 g          120 t
ORIGIN      
        1 cggatggagg ataagcgcca ggaagagcat atcaagctcg caacggaata tggcaaaaca
       61 cagctgaaca tgggacacct ggtcaacagc cggcgtgaaa agcgaggtgc cggttttgaa
      121 ctgcgaacgg atgagcatgg ggctgtacgt gcagcaaaag ggctgttcct gacggcagat
      181 gcccaggcca gagcccaggg gccggtgctg gaaatggcgg ccgcgataag tcagattaac
      241 caggctaaca gccagatgca ggccctgaac agcgctgccg aagccgccgg cgccctgctc
      301 agtgatatca atactcatat taggtttgtg accgacaaaa tcagggaact tcagtctgcg
      361 gtcctgctgg gtagtgcgcc gcagggggtg gccctgacct caggtgaaca ccttcagctc
      421 tgcagtaccc gtaacaccat gatcaatgca ggccagcatc ttgatatcgg tgcgatgaaa
      481 aatctgtcgg taacggtgga aaaagccctt ggtctgtttg tgcataaaga cggggcgaag
      541 ctggtagcca accagggaga tattgagatt caggctcagc acaacacgat ggcgctgttc
      601 tctgaaaaac agctgacggt gaccagtagt gaagacgaaa ttattatcag caccccggaa
      661 ac
//
LOCUS       DAZKFU020000675          662 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZKFU020000675 DAZKFU020000000
VERSION     DAZKFU020000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.685"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>662
                     /locus_tag="ACHOQQ_RS26445"
                     /old_locus_tag="pgaptmp_005289"
     CDS             <1..>662
                     /locus_tag="ACHOQQ_RS26445"
                     /old_locus_tag="pgaptmp_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HGU4473388.1"
                     /translation="RMEDKRQEEHIKLATEYGKTQLNMGHLVNSRREKRGAGFELRTD
                     EHGAVRAAKGLFLTADAQARAQGAVLEMAAAISQINQANSQMQALNSSAEAAGALLSD
                     INTQIRFVTDKIRELQSAVLLGSAPQGVALTSGEHLQLCSTRNTMINAGQHLDIGAMK
                     NLSVTVEKALGLFVHKDGAKLVANQGDIEIQAQHNTMALFSEKQLTVTSSEDEIIIST
                     PET"
BASE COUNT          181 a          161 c          200 g          120 t
ORIGIN      
        1 cggatggagg ataagcgcca ggaagagcat atcaagctcg caacggaata tggcaaaaca
       61 cagctgaaca tgggacacct ggtcaacagc cggcgtgaaa agcgaggtgc cggttttgaa
      121 ctgcgaacgg atgagcatgg ggctgtacgt gcagcaaaag ggctgttcct gacggcagat
      181 gcccaggcca gagcccaggg ggcggtgctg gaaatggcgg ccgcgataag tcagattaac
      241 caggctaaca gccagatgca ggccctgaac agctctgccg aagccgccgg cgccctgctc
      301 agtgatatca atacccagat taggtttgta accgacaaaa tcagggaact tcagtcagcg
      361 gttctgctgg ggagtgcgcc gcagggggtg gccctgacct caggtgaaca ccttcagctc
      421 tgcagtaccc gtaataccat gatcaatgca ggtcagcatc ttgatatcgg tgcgatgaaa
      481 aatctgtcgg taacggtgga aaaagccctt ggtctgtttg tgcataaaga cggggcgaag
      541 ctggtagcca accagggaga tattgagatt caggctcagc acaacacgat ggcgctgttc
      601 tctgaaaaac agctgacggt gaccagtagt gaagacgaaa ttattatcag caccccggaa
      661 ac
//
LOCUS       DAZKFU020000676          661 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZKFU020000676 DAZKFU020000000
VERSION     DAZKFU020000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.686"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>661
                     /locus_tag="ACHOQQ_RS26450"
                     /old_locus_tag="pgaptmp_005290"
     CDS             <1..>661
                     /locus_tag="ACHOQQ_RS26450"
                     /old_locus_tag="pgaptmp_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4473389.1"
                     /translation="DGRVTEQLNPAGLSYTYCYEQNRITITDSLDRREVLHTQGEGGL
                     KRVVKKEHADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASA
                     FYYNHHSQLTSATGPDGLELRREYDESGRLIQETAHNGDITRYRYDNPHSDLPCATDD
                     ATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQL
                     IA"
BASE COUNT          164 a          188 c          201 g          108 t
ORIGIN      
        1 gcgacgggcg ggtgacagag cagcttaacc cggcgggctt aagctacacc tactgttatg
       61 agcaaaaccg catcaccatc accgacagcc tggaccgccg tgaagtgctg cacacgcagg
      121 gcgaaggcgg gctgaagcgg gtggtgaaaa aggaacacgc ggacggcagc gtcacgcaga
      181 gtcagtttga tgcggtgggc aggcttaagg cacagacgga tgccgcaggc cggacaacag
      241 aatacagccc ggatgtggtg acgggactca tcacgcgcat caccacaccg gatggcaggg
      301 catcggcatt ttactataac caccacagcc agttaacgtc agccaccggg cctgacgggc
      361 tggaattgcg ccgggaatat gatgaatccg gccgtctgat tcaggaaact gcacacaacg
      421 gcgatatcac ccgctaccgt tatgataatc cacacagtga cttaccctgc gcaacggacg
      481 atgccaccgg cagccggaaa accatgacgt ggagccgtta cggccagttg ctgagcttca
      541 ccgactgttc cggttatgta acccgttatg accatgaccg tttcgggcag atgacggcgg
      601 tgcaccgcga ggaagggctg agtcagtacc gcgcatacga cagccgtgga cagttaattg
      661 c
//
LOCUS       DAZKFU020000677          661 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZKFU020000677 DAZKFU020000000
VERSION     DAZKFU020000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.687"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>661)
                     /locus_tag="ACHOQQ_RS26455"
                     /old_locus_tag="pgaptmp_005291"
     CDS             complement(<1..>661)
                     /locus_tag="ACHOQQ_RS26455"
                     /old_locus_tag="pgaptmp_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU4473390.1"
                     /translation="VTAGNGATVAPTVITEPDGTVEISVTSQTAGISTVTASINSSSL
                     SRDVMFIADIRTAQIADLVVIKDGSEADGATANTLRARVTDAFGNTLAGQTVSVMADN
                     GATVASTVTTGQDGTVEISVTSQTAGTSVVTTSINSSNASRNVTFIADVRTAQIADLV
                     VIKDGSEADGATANTLRARVTDAFGNALAGQTVSVSADNSAMVTPTVTTQPDGTVEIS
                     VT"
BASE COUNT           99 a          238 c          176 g          148 t
ORIGIN      
        1 tggtgacact gatctccacc gtgccatccg gctgcgtggt caccgtcgga gtgaccattg
       61 cgctgttgtc tgccgatacg ctgaccgtct gcccggcaag cgcattgccg aacgcatcgg
      121 tcaccctcgc ccgcagcgta ttcgccgtcg caccgtccgc ctctgaacca tccttaatga
      181 ccaccagatc cgcgatctgc gccgtcctga catccgcaat aaacgtcacg ttcctgctcg
      241 cgttgctgct gttgatgctg gtggtgacca cactggtgcc cgccgtctgg ctggtgactg
      301 agatctccac cgtgccgtcc tgccctgtgg tgaccgtcga agcgaccgtt gcgccgttgt
      361 ccgccatcac gctgaccgtc tgcccggcaa gcgtattacc gaacgcatcg gtcaccctcg
      421 cccgcagcgt gttcgccgtc gcaccgtccg cctctgaacc atccttaatg accaccagat
      481 ccgcgatctg cgccgtcctg atatcggcga taaacatcac gtcccggctc aggctgctgc
      541 tgttgatgct ggcagtgact gtactgatcc ccgccgtctg gctggtgaca ctgatttcca
      601 ccgtgccgtc cggctctgta atcaccgtcg gggcaaccgt tgcgccgttg cctgctgtca
      661 c
//
LOCUS       DAZKFU020000678          660 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZKFU020000678 DAZKFU020000000
VERSION     DAZKFU020000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.688"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>660
                     /locus_tag="ACHOQQ_RS26460"
                     /old_locus_tag="pgaptmp_005292"
     CDS             <1..>660
                     /locus_tag="ACHOQQ_RS26460"
                     /old_locus_tag="pgaptmp_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473391.1"
                     /translation="INSLYEAANDDTRTSHALVKHNYQRLLDKIDSGSDRYSPMEYWR
                     ALHNPDYRDVLQKTIVKHPSDWYFKKDDAIWQPFLNALKKDAPEWKKYSEDFLDKMAW
                     MQDVTTEKLGPLLWHMHPIMFLGCMKKSSGIWPLGKTSERYESGGRGPGVISSGKGDH
                     GGVSYGMYQLSSKMGTVNKYIIMSKYKDLFEGKKPATPEFNEIWKKIALIHKEEFAQD
                     Q"
BASE COUNT          221 a          120 c          151 g          168 t
ORIGIN      
        1 ttatcaactc actctatgag gctgcgaacg atgatacgcg aaccagtcat gcgctggtga
       61 aacataatta tcagcgcctg ctggataaaa ttgatagtgg aagcgaccgt tactctccaa
      121 tggaatactg gagagcactt cataaccccg actatcgtga tgttcttcag aaaacaatcg
      181 ttaaacatcc cagcgactgg tacttcaaaa aagacgatgc aatctggcaa ccatttctga
      241 atgcgctgaa aaaagatgct ccggaatgga agaaatacag cgaagatttt cttgataaga
      301 tggcctggat gcaggatgtg accacggaga aactgggccc tttgttgtgg catatgcatc
      361 cgattatgtt cttaggctgt atgaaaaaat cgtcaggaat atggccactt gggaaaactt
      421 cagagcgtta tgagtctgga ggaagagggc caggtgtaat ttcatctgga aaaggagatc
      481 atggcggtgt gtcatatgga atgtatcaat tatcttctaa gatgggaacg gttaataaat
      541 atattataat gtcaaaatac aaagatttat ttgagggtaa aaaacctgct acacctgaat
      601 tcaatgaaat atggaaaaaa attgctctca ttcacaaaga agaattcgca caagatcagc
//
LOCUS       DAZKFU020000679          660 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZKFU020000679 DAZKFU020000000
VERSION     DAZKFU020000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.689"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          179 a          126 c          166 g          189 t
ORIGIN      
        1 ttgacagctc atcaaaatag aataaatgtt gctgaggttg ctgattctga caagagcgaa
       61 tttagtagaa ttaaaacctc ttcggatttt gatggggcat tatggactgg gataagaaag
      121 cttatttcat tctttaaaaa atagatctat agcgtatcgt tccggcgaac tcatcctgat
      181 gcgttttcgg gggtccatcg gtgaaacata cgaatgatcg ccctctgacg atggatgatt
      241 tattttgttg cacagaagcg atcgctgggc aggtaccgga ggttggctct gaagctctaa
      301 ttgcgtcagg ttgcgaagtg ttgacacggg tattggtgag aactgccccg ggaacgactc
      361 tgggctgatt ctggttctga ttcgctgtca gcccgtgtgg ttactggcct ggctgatttc
      421 ctgtcgtcag gcgtttaact cccgactgca ggtatcactt accaagagaa ggtaaatcct
      481 tttagacgta cagtgaaagt tgtctggtca taatctgaag catctgacaa agttgtgtcc
      541 accaaataat aagtagtggg aaccaaagtg tcatatatac agaaaaatat aactcaccga
      601 tttccttcac gttaatacgc agggggaggt atataacagt ccgttcaggt attggataaa
//
LOCUS       DAZKFU020000680          656 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZKFU020000680 DAZKFU020000000
VERSION     DAZKFU020000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.690"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>656)
                     /locus_tag="ACHOQQ_RS26465"
                     /old_locus_tag="pgaptmp_005293"
     CDS             complement(<1..>656)
                     /locus_tag="ACHOQQ_RS26465"
                     /old_locus_tag="pgaptmp_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473392.1"
                     /translation="VLNADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERV
                     ADEVRAQLATDPQLLAVNAMAAKLGDAQLLNLAMRGTAKTVKSGIVRNATAQGAINAA
                     QGGYSRYQENTALRETAGMDVSPWEGVADATIEGAALGAAMGAPFGAVAGYRGRRQAA
                     EETAMRDAETVQQDDAAPQPESVDPVAQHRESMQGMNREQLLEQYADADMATEGDASA
                     A"
BASE COUNT          125 a          213 c          166 g          152 t
ORIGIN      
        1 gctgcggatg cgtcaccctc tgttgccata tccgcatcag catactgctc cagaagctgc
       61 tcgcgattca tcccctgcat ggattcacga tgctgcgcca ccggatcaac agattctggt
      121 tgcggggctg cgtcgtcctg ctgcacggtt tcagcatcac gcatggcggt ttcttctgcg
      181 gcctgacgtc tgccacgata tccggcaact gcaccgaatg gagcccccat cgcagcacca
      241 agggcagcac cttcgatcgt tgcgtcagcc acgccctccc atggcgatac atccattccg
      301 gcggtctcac gcaatgccgt gttttcctga tagcgtgaat agccgccctg cgccgcatta
      361 atcgccccct gtgcagtggc atttctgaca atgccgcttt taacggtctt cgctgtgccc
      421 cgcatcgcca gattaagcaa ctgagcatcg cccagttttg ccgccatggc attcacagcc
      481 agcaactgag gatcggttgc cagttgcgca cgcacctcat cagccacacg ctcttttgcc
      541 aggtccattt tctggcgatc agtaagctgt gcgtgctgcg ggtcggcgtc aatggacaaa
      601 aacgtctgct gaaatttcgg tgactgcgcc agctcagagt aatccgcatt aagaac
//
LOCUS       DAZKFU020000681          655 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZKFU020000681 DAZKFU020000000
VERSION     DAZKFU020000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.691"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..187)
                     /gene="subB"
                     /locus_tag="ACHOQQ_RS26470"
                     /old_locus_tag="pgaptmp_005294"
     CDS             complement(<1..187)
                     /gene="subB"
                     /locus_tag="ACHOQQ_RS26470"
                     /old_locus_tag="pgaptmp_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HGU4473393.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGG"
     gene            complement(204..>655)
                     /gene="subA"
                     /locus_tag="ACHOQQ_RS26475"
                     /old_locus_tag="pgaptmp_005295"
     CDS             complement(204..>655)
                     /gene="subA"
                     /locus_tag="ACHOQQ_RS26475"
                     /old_locus_tag="pgaptmp_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HGU4473394.1"
                     /translation="PAAYHPVSSVNKKQDPVIRVAALAQYRKGETPVLHGGGVTGSRF
                     GNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATAIVSGVLAAMVSCNPRATATELKRT
                     LLESADKYPSLADKVTEGRVLNAEKAISMFCKKNYIPVRQGRMSEEL"
BASE COUNT          161 a          187 c          125 g          182 t
ORIGIN      
        1 aaccaccagg ttgtttccct tcaatacaaa aataagggtt attatctatt tgccctgtat
       61 gaaactgatc aataacaacg cctgaaaaca tgccatcccg ggcatcgcca gtccactcag
      121 ccatggcagg attaagggat aaacatccca taacacctgc acatacaaaa aacctcttaa
      181 tcgtcatatt cacctccgga attttacagt tcttcactca tccttccctg acggacagga
      241 atataatttt tcttgcagaa catgctaatt gctttttctg cattaagaac tctcccttcc
      301 gtcaccttat ctgcaagtga ggggtatttg tctgcagact ccagcagtgt acgcttcagt
      361 tctgtcgctg ttgcacgggg attacatgag accatcgctg ccagaacgcc ggaaactata
      421 gctgttgctt ccgaggttcc actaccgatc cccgtttttc catctggtct gaggaatgta
      481 atattctgtc ccggtgcggc aatatcaacc cagccgtttc cgaaccgact tccggtgacc
      541 cctccaccat gcaatacagg tgtttctcct ttccggtact gtgccagagc tgccactctt
      601 atcacaggat cctgcttttt attcacactg ctgaccggat ggtaagccgc aggcc
//
LOCUS       DAZKFU020000682          655 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZKFU020000682 DAZKFU020000000
VERSION     DAZKFU020000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.692"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>655)
                     /locus_tag="ACHOQQ_RS26480"
                     /old_locus_tag="pgaptmp_005296"
     CDS             complement(<1..>655)
                     /locus_tag="ACHOQQ_RS26480"
                     /old_locus_tag="pgaptmp_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4473395.1"
                     /translation="NEENPHHVYQPYRLPGQQYDEESGLYYNRNRYYDPLQGRYITQD
                     PIGPVGGWNPYSYVLNNPAQFIDPTGLTQEDNWTYGEVGNSCVTSQRDFTPPPGLADM
                     KASIGAACSRNNASRNCHPVDGSNASAPSDKAAWENIQNATNGKDLSGGGNMFCVGNE
                     DCAVVHSCREIVNARHPAGRVRERVTPLTTSGTATLNGTTVYFYNDPRNGNCPAHEYI
                     "
BASE COUNT          161 a          173 c          141 g          180 t
ORIGIN      
        1 gaatatattc gtgagcagga caattaccat tccggggatc attataaaag taaactgttg
       61 ttccattgag cgttgctgtt cctgacgttg ttaacggagt tacacgttca cgcactcgtc
      121 ctgccgggtg tcgagcatta actatttcac gacagctatg aacaaccgca cagtcctcat
      181 ttcctacaca aaacatattt ccaccaccag ataaatcttt accatttgtc gcattttgaa
      241 tattttccca tgcggcttta tccgatggtg cagaagcatt actaccatct acggggtggc
      301 agtttctgga agcattgttt cttgagcagg cagcgccaat gctggctttc atatccgcaa
      361 gtccgggcgg gggagtaaaa tcgcgttgac tcgtaacaca gctattacct acctcaccat
      421 aggtccaatt gtcctcttga gttaaacctg ttgggtcaat aaactgggct ggattattaa
      481 gcacataact atagggattc caccccccca ccggcccaat cggatcctgg gtaatatacc
      541 tcccctgcaa cggatcatag taccggttgc ggttatagta cagccccgac tcctcatcat
      601 actgctgccc cggcaggcgg tatggctgat acacatgatg cgggttctcc tcatt
//
LOCUS       DAZKFU020000683          655 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZKFU020000683 DAZKFU020000000
VERSION     DAZKFU020000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.693"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..552
                     /locus_tag="ACHOQQ_RS26485"
                     /old_locus_tag="pgaptmp_005297"
     CDS             <1..552
                     /locus_tag="ACHOQQ_RS26485"
                     /old_locus_tag="pgaptmp_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HGU4473396.1"
                     /translation="DIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETI
                     EKHLAWLDGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVF
                     QVFHYGLISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRM
                     GEPDPDVMSGKKRSVYSPPVKAG"
BASE COUNT          171 a          133 c          180 g          171 t
ORIGIN      
        1 gacatcatca gtatgcccag ggctattgcg tttgcgggtc aggcaaccag agattcagcc
       61 agggataatc cttatgcaga tatgatgtta gtcaggctgg aaaatgccct gacgaaggca
      121 actgaaacaa ttgaaaagca tcttgcgtgg cttgacggga tccttaaaaa cctgccggga
      181 cagatctccc tttctgatat taaatcctct tcgccggtaa atattggcgt gtacagtagt
      241 tctccggtcg gttatcgatg tgtatggctt ctgatcggat atgatcgact ggtcatgaag
      301 gtatttcagg tatttcatta cggacttatt tcccgcacaa aacgggatga gttgttgaat
      361 cagggagggt atgcggtacg caggatttac gggattattc aaaattataa atcagtcagt
      421 gttacacgtg ctgatatttt gtccggcaca gcccgtggaa aggaggcggt taaccgcatg
      481 ggggagccgg atcctgacgt gatgagcggt aagaaacgtt ctgtttattc accaccggtt
      541 aaagcggggt gagtatgcaa ctctttctgt gtgaaaaacc ttctcaggcg aaagatatcg
      601 cccgtgaact ggcgctcagt aagcggggcg acggatacat cggagatgac aatat
//
LOCUS       DAZKFU020000684          649 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZKFU020000684 DAZKFU020000000
VERSION     DAZKFU020000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.694"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>649)
                     /locus_tag="ACHOQQ_RS26490"
                     /old_locus_tag="pgaptmp_005298"
     CDS             complement(<1..>649)
                     /locus_tag="ACHOQQ_RS26490"
                     /old_locus_tag="pgaptmp_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HGU4473397.1"
                     /translation="KRDEWDATPPIQRVTQSIDARLIQLTAKPDKSGGDFKEIDLLTR
                     QLKKLNDGQGEQAVSGKKPRRRKLKNHFTEEQIVALREKILPSLAWHQREWYDNRHHR
                     NRMILKSRQIGATWYFAREALLDALRDDVSAGYQRNQIFLSASRRQAYQFKHFIQQAA
                     LEVDVELKGGDKIVLSNGAELHFLGTSAATAQSYTGHLKFDEFFWVSNFVELRKVA"
BASE COUNT          140 a          171 c          172 g          166 t
ORIGIN      
        1 gcaaccttcc tgagttccac aaagttactg acccaaaaaa attcatcaaa tttaaggtgt
       61 ccggtatacg actgagcggt tgctgccgat gtgccaagga agtgaagctc tgcaccgtta
      121 gacaaaacaa tcttgtcacc ccctttcagt tcgacatcaa cttccagcgc cgcctgctga
      181 ataaagtgtt tgaactgata cgcctggcga cgggatgcgg acaaaaatat ctggttgcgc
      241 tggtacccgg cggagacatc atcacgcagt gcatcaagga gtgcttcgcg cgcaaaatac
      301 caggtcgcac caatctgacg cgacttcagg atcatgcggt tgcggtgatg gcggttgtcg
      361 taccattcgc gctgatgcca ggccagtgac ggcaggattt tttccctgag tgcgacaatc
      421 tgctcttcgg tgaagtgatt tttcagtttg cgcctgcggg gcttcttacc ggaaacagcc
      481 tgttctccct gaccgtcatt cagttttttc agctggcgcg tcagcaggtc aatttcctta
      541 aagtcaccgc cgcttttatc cggtttggcg gtgagctgta tcaggcgtgc atcaatggac
      601 tgggtgaccc gctggatcgg gggagtggca tcccactcat cgcgttttt
//
LOCUS       DAZKFU020000685          644 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZKFU020000685 DAZKFU020000000
VERSION     DAZKFU020000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.695"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          217 a          112 c          128 g          187 t
ORIGIN      
        1 cttaatgatg tagttcaaaa tatcaggcgt tggctaaaag aaaatgaaaa tatggtgaaa
       61 gctattaaga aaattgatac taataacatg gcgaataaat aaacggatca agtcgttcct
      121 gtgacaatga ggttatacga aaaagacgct tacgcattag aaatctggtg tactttaatt
      181 aaaccagtgt cgctgatgtg taagcgtcga cgttgggaga cgattaaatt gttatatcac
      241 cgctacgccc tttccagacg gcgtataaga gtgcaatatg ttgagttttc cgagtgaatg
      301 gtgcatttta ttaaccgagc catttgaata agccaacgca ataaaacaga ctggaataat
      361 aagtctgtat ttattgttca tcacaatcgc actctacggt tttatcctgc tgaaatgaga
      421 cagcctccag cgtttgtgga tgatccttga acgagagtca tggtctggtg tcaggattca
      481 caaatgacct gtttcctgac atggatataa acccaacata gagtgcggta gttaatctta
      541 aaaatctata gtcggctact cagcacaaaa catcattatt gattctttat aagaaagaat
      601 ctcgccatca tttttatata aatcactatt aaggaaatcc agaa
//
LOCUS       DAZKFU020000686          641 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZKFU020000686 DAZKFU020000000
VERSION     DAZKFU020000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.696"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>641
                     /locus_tag="ACHOQQ_RS26495"
                     /old_locus_tag="pgaptmp_005299"
     CDS             <1..>641
                     /locus_tag="ACHOQQ_RS26495"
                     /old_locus_tag="pgaptmp_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4473398.1"
                     /translation="QLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEGGLKRVVKK
                     EHADGSVTQSQFDAVGRLKAQTDTAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHH
                     NQLTSATGPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRK
                     TMTWSRYGQLLSFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIA"
BASE COUNT          159 a          186 c          191 g          105 t
ORIGIN      
        1 cagctaaacc cggcaggctt aagctacacg tatcagtatg agaaagaccg catcaccatc
       61 accgacagcc tggaccgccg tgaagtgctg cacacgcagg gcgaaggcgg gctgaagcgg
      121 gtggtgaaaa aggaacacgc ggacggcagc gtcacgcaga gtcagtttga cgccgtgggc
      181 aggcttaagg cacagacaga tacggcaggc cggacaacag aatacagccc ggatgtggtg
      241 acgggcctca tcacgcgcat caccacgccg gatggcaggg catcggcgtt ttactataac
      301 caccacaacc agttaacgtc agccaccggg cctgacgggc tggaattgcg ccgggaatat
      361 gatgaatccg gccgtctgat tcaggaaacc gcccctgacg gcgatatcac ccgctaccgt
      421 tatgataatc cacacagtga cttaccctgc gcaacggacg atgccaccgg cagccggaaa
      481 accatgacgt ggagccgtta cggccagttg ctgagcttca ccgactgttc cggttatgta
      541 acccgttatg accatgaccg tttcggtcag gtgacggcgg tgcaccgcga ggaagggctg
      601 agtcagtacc gcgcatacga cagccgtgga cagttaattg c
//
LOCUS       DAZKFU020000687          637 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZKFU020000687 DAZKFU020000000
VERSION     DAZKFU020000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.697"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>637
                     /locus_tag="ACHOQQ_RS26500"
                     /old_locus_tag="pgaptmp_005300"
     CDS             <1..>637
                     /locus_tag="ACHOQQ_RS26500"
                     /old_locus_tag="pgaptmp_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HGU4473399.1"
                     /translation="YQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLL
                     KNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDE
                     SGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAV
                     DKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEG"
BASE COUNT          195 a          118 c          142 g          182 t
ORIGIN      
        1 tatcaactgg tatcggaacg cgcggtaaaa cgtgccgata gccgctttga acttattcag
       61 aaaaacgttg gctatttctt taaagatatt gaacgttcgg ccctgacatt aaaggactca
      121 ctgtatttat taaaaaatac agaggagatt caacgcgctg tgatcctgaa aatggaaatg
      181 atgccatttt tagactcggt gggactggta cttgatgata ataaatatta tctcttttcg
      241 cggagggcga acgataaaat cgttgtttat catcaggaac aagtaaatgg accgcttgtc
      301 gacgagtcag ggcgggttat ttttgccgat tttaacccat cgaaacgacc gtggtcggtg
      361 gcttcagatg actctaacaa cagctggaat ccggcataca attgctttga tcgtccgggt
      421 aaaaaatgta tctcttttac gctacgcatc aacggcaaag atcacgattt gttagcggtg
      481 gataaaattc atgtcgattt aaactggcga tatctgaacg agtatcttga tcaaatcagc
      541 gctaatgatg aagttctatt tttgaaacaa ggccatgaga tcattgccaa gaatcaactc
      601 gctcgtgaaa aactgattat ttataatagc gaaggta
//
LOCUS       DAZKFU020000688          627 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZKFU020000688 DAZKFU020000000
VERSION     DAZKFU020000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.698"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          195 a          151 c          127 g          154 t
ORIGIN      
        1 agtttatttt ttttcttcat gtaagccctt accctgaaga gcggagtttt acggtgcgct
       61 aagtaaaacg taagtgctgt ccctagtcgt ggattgctgt agctgagagt cggaaacgtg
      121 ctcaaaacgt gcctgacagc gagaacgcta acaaattaaa aactattgcc tgaaaaaaat
      181 cgcgttaaaa agtgtatgag tgcatttcca gcgagcacaa aagccacatt cagtgaattg
      241 cctcagcaga gccccagaaa tacactcaac ccgataaaaa tacttgccga aaattccggt
      301 gatatttcaa ccctcaaggg ggatttttta tgcaatcacc gcaacaaaaa ctggacatac
      361 tgaaatacac gatgccctgt taccagtgcg tttctggcat caatgtagtt tgtagggatg
      421 attacagtca ggcatgtgcc agaaacacgc tcaattctca atctattcct tgcaaccgac
      481 caactccgtg accttgatat ccaccgcagt ctgaagcata caaccgggcc cgtgcaaaat
      541 acggacttcc ggaaatctga aaaattactc ttatcagaga agagcacaga aacacgatcg
      601 gcatcactgt ttctgcaaac ttccctg
//
LOCUS       DAZKFU020000689          626 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZKFU020000689 DAZKFU020000000
VERSION     DAZKFU020000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.699"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>626
                     /locus_tag="ACHOQQ_RS26505"
                     /old_locus_tag="pgaptmp_005301"
     CDS             <1..>626
                     /locus_tag="ACHOQQ_RS26505"
                     /old_locus_tag="pgaptmp_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGU4473400.1"
                     /translation="WMRDCHSSDDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMH
                     SLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFA
                     VKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTF
                     EWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIR"
BASE COUNT          206 a          113 c          150 g          157 t
ORIGIN      
        1 tggatgcgtg attgtcattc ttcagatgat aaagacgtca ttgcaattga tggaaaaacg
       61 ctccggcatt cttatgacaa gagtcgccgc aggggagcga ttcatgtcat tagtgcgttc
      121 tcaacaatgc acagtctggt catcggacag atcaagacgg atgagaaatc taatgagatt
      181 acagctatcc ctgaacttct taacatgctg gatattaaag ggaaaattat cacaactgat
      241 gcgatgggtt gccagaaaga tatcgcagag aagatacaaa aacagggcgg tgattattta
      301 ttcgcggtaa aaggtaacca ggggcggctt aataaagcct ttgaggaaaa atttccgctg
      361 aaagaattaa ataatccaga gcatgacagt tacgcaatta gtgaaaagag tcacggcaga
      421 gaagaaatcc gtcttcatat tgtttgcgat gtccctgatg aacttattga tttcacgttt
      481 gaatggaaag ggctgaagaa attatgcgtg gcagtctcct ttcggtccat aatagcagag
      541 caaaagaaag agccagaaat gacggtcaga tattatatca gttctgctga tttaaccgct
      601 gagaaattcg ccacagcgat ccgaaa
//
LOCUS       DAZKFU020000690          625 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZKFU020000690 DAZKFU020000000
VERSION     DAZKFU020000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.700"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>625
                     /locus_tag="ACHOQQ_RS26510"
                     /old_locus_tag="pgaptmp_005302"
     rRNA            <1..>625
                     /locus_tag="ACHOQQ_RS26510"
                     /old_locus_tag="pgaptmp_005302"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          171 a          135 c          196 g          123 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact
      601 atgtgttgtt gggtagggga gcgtt
//
LOCUS       DAZKFU020000691          625 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZKFU020000691 DAZKFU020000000
VERSION     DAZKFU020000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.701"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>625
                     /locus_tag="ACHOQQ_RS26515"
                     /old_locus_tag="pgaptmp_005303"
     CDS             <1..>625
                     /locus_tag="ACHOQQ_RS26515"
                     /old_locus_tag="pgaptmp_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4473401.1"
                     /translation="HGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTINKNGRQ
                     VIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGWQVIKE
                     GGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSVVAGKA
                     DNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGV"
BASE COUNT          159 a          158 c          203 g          105 t
ORIGIN      
        1 acatggcgga ctggcaaccg gaaccatcgt caacaccggt gcagaaggtg gtccggagtc
       61 tgaaaatgtg tccagcggtc agatggtcgg agggacggct gaatccacca ccatcaacaa
      121 aaatggccgg caggttatct ggtcttcggg gatggcacgg gacaccctca tttacgccgg
      181 tggtgaccag acggtacacg gagaggcaca taacacccgg ctggaggggg gtaaccagta
      241 tgtacacaac ggtggcacgg caacagagac gctgataaac cgtgatggct ggcaggtgat
      301 taaggaagga ggaactgccg cgcataccac catcaaccag aaaggaaagc tgcaggtgaa
      361 tgccggcggt aaagcgtctg atgtcaccca gaacacgggc ggtgcactgg ttaccagtac
      421 agctgcaacc gtcaccggta caaaccgcct gggagcattc tccgttgtgg cgggtaaggc
      481 tgataatgtc gtactggaaa atggcggacg tctggatgtg ctgagcggac acacagccac
      541 taatacccgt gtggatgatg gcggaacgct ggatgtccgc aacggtggtg ccgccaccac
      601 cgtatccatg gggaatggcg gtgta
//
LOCUS       DAZKFU020000692          624 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZKFU020000692 DAZKFU020000000
VERSION     DAZKFU020000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.702"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            144..>624
                     /locus_tag="ACHOQQ_RS26520"
                     /old_locus_tag="pgaptmp_005304"
     CDS             144..>624
                     /locus_tag="ACHOQQ_RS26520"
                     /old_locus_tag="pgaptmp_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HGU4473402.1"
                     /translation="MNKIYALKYSSLTGGLIAVSELSKKVTGKTGRRLMTVSLVLSVT
                     LSVLPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEINDNNGNKVGVLDVPMLDFS
                     SLSRDGHTTLIHPGYVVSAKHGGLQSVSSATFGYDQIYKIVDNNLAGIDFSAPRLNKL
                     "
BASE COUNT          192 a          108 c          124 g          200 t
ORIGIN      
        1 gtttgttttc cacaggaaaa atcttaagta tggacaggta ttcctgatgt catttaaaat
       61 agattcaggg tgagtatcca gtactcactc tttccggaca gacaatacat tttaatacca
      121 tataattata tagcaggaat tatatgaata aaatatacgc gcttaaatat agctccctta
      181 ctggtgggct tatagctgtg tcagaattaa gtaagaaagt cacaggaaaa accggcagaa
      241 gattaatgac ggtttccctg gtattatcag tgactctttc tgttttaccg ggtaaagcat
      301 caacggtcag cgcagaaata ccatatcaga cttttcgtga ctttgctgaa aataaaggtg
      361 tgtttacccc cggagtgaca ggaattgaaa taaacgacaa caatggaaat aaagttgggg
      421 ttcttgatgt tcccatgctt gatttttcca gtctttctcg tgatggtcat accacattga
      481 ttcatcctgg ttatgttgta tcggctaaac atggtggttt acaaagtgtt tcatcagcaa
      541 cttttggtta tgaccagata tataaaatag ttgataataa ccttgctggt atagattttt
      601 ctgccccacg attaaataag cttg
//
LOCUS       DAZKFU020000693          621 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZKFU020000693 DAZKFU020000000
VERSION     DAZKFU020000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.703"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..342
                     /locus_tag="ACHOQQ_RS26525"
                     /old_locus_tag="pgaptmp_005305"
     CDS             <1..342
                     /locus_tag="ACHOQQ_RS26525"
                     /old_locus_tag="pgaptmp_005305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473403.1"
                     /translation="QHDHIWYLTGYSRKDASKHVVFSDNEPNQNTIDTFERHYKKNRP
                     LINESIWNGQPDGLACSISHFMKFIDNPKKLNLIIDIDQRVANTSDMIDSLLLLAECG
                     MDVIYAPHTLT"
     gene            320..523
                     /locus_tag="ACHOQQ_RS26530"
                     /old_locus_tag="pgaptmp_005306"
     CDS             320..523
                     /locus_tag="ACHOQQ_RS26530"
                     /old_locus_tag="pgaptmp_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm52 family immunity protein"
                     /protein_id="HGU4473404.1"
                     /translation="MPHILLPELIPEAAKIVPVFEGEKQKGTIVVSTEDVFDGNNKEH
                     IGKANDIEIRLLDLGLLPLLTEL"
BASE COUNT          208 a          116 c          119 g          178 t
ORIGIN      
        1 cagcacgatc acatatggta cttaactggc tactcaagaa aggacgcttc aaagcatgtc
       61 gtgtttagtg ataatgaacc gaaccaaaat acaatagata cctttgagcg gcattataaa
      121 aagaatcgtc ctttaattaa tgaatctata tggaatggac aacccgatgg gctggcttgc
      181 tcaatatcac attttatgaa atttattgat aaccctaaaa aactaaatct tatcatagat
      241 attgatcaga gagttgccaa tacttcggat atgattgatt ccttgcttct gttggctgaa
      301 tgtgggatgg atgttatata tgccccacat actcttacct gaattaatcc ctgaagccgc
      361 aaaaatagta ccggttttcg agggcgagaa gcaaaaggga actattgttg tttcaaccga
      421 agacgtattt gacggaaata ataaggaaca catcggaaaa gctaatgata ttgaaataag
      481 acttctggat cttggactgc tacctttgct aacagagctt taatccaacc ttatcactca
      541 tgggtagatg acgggacagt tacacccgtt aatggcctta tttttaacac atcagataaa
      601 attgaatgct caccattaag a
//
LOCUS       DAZKFU020000694          618 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZKFU020000694 DAZKFU020000000
VERSION     DAZKFU020000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.704"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>618)
                     /locus_tag="ACHOQQ_RS26535"
                     /old_locus_tag="pgaptmp_005307"
     CDS             complement(<1..>618)
                     /locus_tag="ACHOQQ_RS26535"
                     /old_locus_tag="pgaptmp_005307"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4473405.1"
                     /translation="ETYQSTFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFE
                     KLAAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYY
                     PLADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTAD
                     SISSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHN"
BASE COUNT          177 a          138 c          108 g          195 t
ORIGIN      
        1 aattatgaat aacctcttcg aatctttgag ttacgccatc ccaactgtag tgatcaaaca
       61 caaaatcctg accttgcttt gcaactgctg ttaattcagg attcgctaac gtttttaaaa
      121 tatcgctgct tatggagtct gcagtcatag gttcttttaa atggaatccc gtagtatttt
      181 ctttgacaaa ttccgtcatc cccccacgcg tactcactaa aacaggctta ccagcaccca
      241 tagcttcaat tgccaccatg caaaatggtt cctggaactg ggaaggaatg acgactaaat
      301 ctgccagtgg gtaataacaa tgcatctttt caggcggtat gcttcctaac ataatgcaac
      361 gatccttcaa acgttttgca atttccctga cattgcgttg atatgcacct ttgtcacttt
      421 tactcatttc tgtataatca ccaatcacaa ctaattttaa atttttgtga gcagcggcca
      481 atttttcgaa tgattgcatc aacagcaata tgcctttgtc agggacgata cgcccagcat
      541 aaaaaataat tttctcttca ggggagatat taagttcgct tctagtgatc ggctgaaaag
      601 tagattgata ggtttcta
//
LOCUS       DAZKFU020000695          615 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZKFU020000695 DAZKFU020000000
VERSION     DAZKFU020000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.705"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..355)
                     /locus_tag="ACHOQQ_RS26540"
                     /old_locus_tag="pgaptmp_005308"
     CDS             complement(<1..355)
                     /locus_tag="ACHOQQ_RS26540"
                     /old_locus_tag="pgaptmp_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4473406.1"
                     /translation="MRGSVIIPVALHALVAVSTLLWLLWYFIPAGNVFNGLATLLILL
                     LAGWSVFKNSRSEKQKLTSDVTLTDAEPALSDTRGPVVLVCGDMQEALFQDGPLRKTA
                     RGWWLRVGDVSRLTDV"
     gene            complement(355..>615)
                     /locus_tag="ACHOQQ_RS26545"
                     /old_locus_tag="pgaptmp_005309"
     CDS             complement(355..>615)
                     /locus_tag="ACHOQQ_RS26545"
                     /old_locus_tag="pgaptmp_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HGU4473407.1"
                     /translation="FQGVYGLKAVSQSQRDEVMKALSERVSLPDGGLSLVVYRMEKHR
                     WRLIRSVWFWTVLAVILTGVIWWGGYLWLQSLLSSQIPELHG"
BASE COUNT          171 a          184 c          150 g          110 t
ORIGIN      
        1 caacgtcagt taagcgactg acgtcaccga cccgcagcca ccatccccgg gctgttttac
       61 gcagcggacc gtcctgaaac aacgcttcct gcatgtctcc acataccagt acaacagggc
      121 cccgggtgtc ggataatgcc ggttcagcat ccgtcagggt aacgtcagat gtgagctttt
      181 gcttttcact ccggctgttt ttaaaaacac tccagcctgc cagaagaagg atcagcagag
      241 tagccagacc gttaaaaacg ttgcctgcag ggatgaaata ccacagcaac cacagcaacg
      301 tggaaacggc caccagggca tgcagggcta ccggaatgat gacagatcca cgcatcagcc
      361 atgcaactcc ggtatttgtg atgacaaaag agactgaagc cagaggtacc ccccccacca
      421 gataacgccg gtaagaatga cggcgagcac tgtccagaac cagactgagc gtatcagacg
      481 ccagcgatgt ttctccatcc ggtaaacaac cagggacaga ccaccatcag gcagagaaac
      541 acgttcagaa agggctttca tgacctcatc acgctgtgac tggctgactg ctttcaggcc
      601 gtacaccccc tgaaa
//
LOCUS       DAZKFU020000696          613 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZKFU020000696 DAZKFU020000000
VERSION     DAZKFU020000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.706"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>613
                     /locus_tag="ACHOQQ_RS26550"
                     /old_locus_tag="pgaptmp_005310"
     CDS             <1..>613
                     /locus_tag="ACHOQQ_RS26550"
                     /old_locus_tag="pgaptmp_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
                     /protein_id="HGU4473408.1"
                     /translation="LVSDDAMINELSSELVSCLGNGKFTPISEGGKLLNMLSEFKLLR
                     EQCFRWGNYTLLFENYGAYDKTGSITIEKSQGEGTLPIRHKLEFISTNIAELLDKLTK
                     ITDARLCKGFSDWASSVKEGASNDFKENVDRALVRMFKCVKLHSNELDLSYLFLGSVP
                     PLPEWIEMLSLVYNELDSIQVPESCKELEVDFNNLTEFPQVPDG"
BASE COUNT          196 a           89 c          141 g          187 t
ORIGIN      
        1 agttagtatc tgatgatgca atgataaatg aattatcaag tgagttagtc agttgtcttg
       61 gaaatggtaa gtttacacca attagtgaag gcggcaaatt attgaatatg ctgtcagaat
      121 ttaagttatt gagagagcaa tgtttcaggt ggggtaatta tactctattg tttgaaaatt
      181 atggggctta tgataagacg ggatctatca cgatagaaaa aagtcagggg gaggggactt
      241 tacccattcg gcataaatta gagtttatat cgaccaatat tgcagagttg ctggacaagt
      301 taaccaaaat tacagatgcc agactttgca aaggtttcag tgactgggct agttcagtca
      361 aagaaggcgc atcgaatgac ttcaaagaaa atgtggatag agcattggtg agaatgttta
      421 aatgtgttaa gcttcacagt aatgaacttg acttatcata cctatttttg ggttctgtgc
      481 cgcctcttcc tgagtggatt gaaatgctta gccttgttta taatgaactt gattcaatac
      541 aggtgcccga atcatgcaaa gaattagaag tcgatttcaa taaccttaca gaatttccac
      601 aagtacctga tgg
//
LOCUS       DAZKFU020000697          613 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZKFU020000697 DAZKFU020000000
VERSION     DAZKFU020000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.707"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHOQQ_RS26555"
                     /old_locus_tag="pgaptmp_005311"
     CDS             complement(<1..242)
                     /locus_tag="ACHOQQ_RS26555"
                     /old_locus_tag="pgaptmp_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU4473409.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFEL"
BASE COUNT          213 a          131 c          127 g          142 t
ORIGIN      
        1 agttcaaacc aggtcaccac acctttcacc cggcgctgta catcatcacc ctgccatatt
       61 gtcaggtagg ccattttgtc cagcacctgc gcaaattcaa gggagagaaa ctgctggctg
      121 accagggaga gatcgaggga aaagagcgaa gagagtgact gggtgagatg aaaggaaacc
      181 accgcaaaag cgtccggtgg caggccgtcc acttccagtg tgaaacgtaa tccggttgac
      241 atagcaacct ccggtaaaat gaataataat ccgcaccgta caactgcgga taatgagata
      301 agcgggacga cttaagacgt tattagggaa ggaacaaaaa atgctatgaa tatgtgagaa
      361 ttttgtttaa atattacttt acagaaacgg gtgtctgttc agtcaaaaaa ataatcacaa
      421 gctcaactta ttgtttcctg acgttattca ggacaggtgc gcacatcaca gcgagtaaca
      481 aaaacgcaaa gtaaaaccaa tattcataat atgtttttat tcacaactaa ataacccaat
      541 taacatcatt aaaatgaatg attgcttaaa aaatatatgc taccgctcca ccctgataac
      601 atgatctgca cat
//
LOCUS       DAZKFU020000698          613 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZKFU020000698 DAZKFU020000000
VERSION     DAZKFU020000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.708"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>613
                     /locus_tag="ACHOQQ_RS26560"
                     /old_locus_tag="pgaptmp_005312"
     CDS             <1..>613
                     /locus_tag="ACHOQQ_RS26560"
                     /old_locus_tag="pgaptmp_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HGU4473410.1"
                     /translation="KVPLKAYYSSPEDIQKHIPFELEQQFDNLQKNPPPGTCVVACDK
                     FGEALSVFFHRMEKEKLTHMAAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPNATN
                     TAVRYKANNCDSFGSLRSFMNIKQAQKKWVITEICSDCVGITPYLPREQAHLLSGINN
                     ELQPPLSPSALHLLIQMGMHENIVLFFDKFKNSQEMTASKVLAA"
BASE COUNT          209 a          124 c          108 g          172 t
ORIGIN      
        1 gaaagttcca ttaaaggctt actattcatc tccggaagat atacaaaaac atattccatt
       61 cgagcttgag cagcaatttg acaatctaca aaaaaatcca ccacccggta catgcgttgt
      121 tgcctgtgat aagtttgggg aggcattatc agtctttttt cacagaatgg aaaaagagaa
      181 gttaacgcat atggcggcaa tcgttcaatc tcaaacacac gctatggccg tccgcttgag
      241 aatcaaaaaa acgcctgctg gtgaaacaga atatgttgta tccttttatg atcctaatgc
      301 aaccaatact gcagtacgct ataaagcaaa caactgtgat tcttttgggt cattgcgatc
      361 ctttatgaat attaaacagg cacaaaaaaa atgggtaatc acagaaattt gctccgactg
      421 tgtaggaata actccttatc tccctcgaga acaagcccat ttattaagtg gtataaataa
      481 tgagttgcaa ccaccattat caccatcagc attacatcta ttaatacaaa tggggatgca
      541 tgaaaacatt gttctttttt ttgataaatt taaaaactcc caagaaatga cagcatcaaa
      601 ggttcttgct gcg
//
LOCUS       DAZKFU020000699          609 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZKFU020000699 DAZKFU020000000
VERSION     DAZKFU020000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.709"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            399..>609
                     /locus_tag="ACHOQQ_RS26565"
                     /old_locus_tag="pgaptmp_005313"
     CDS             399..>609
                     /locus_tag="ACHOQQ_RS26565"
                     /old_locus_tag="pgaptmp_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HGU4473411.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTR"
BASE COUNT          144 a          172 c          147 g          146 t
ORIGIN      
        1 tatgcccaca gcgtcagcgg cagcagcgct gaaggctata acggtcaggc cacgctgaat
       61 gtgactttct gataatcagg tcactcccct tcaggccccg gtcatcatga ccggggcttt
      121 ttactttcag ggagcaacag atatgccggg ttgtacagca cgtttattgc cgaaggctct
      181 tttgctgcca aatggcatct gaatcgatca cagtctggcc ggttattacc cgatcccgat
      241 ggggttattg ggtgatctcc ttccataaaa atacgcagct cgccagtgaa gggcttctca
      301 gacaataaac gtcattcatt tcccccatca ggccgcgtct tctccgggag acgcggcttt
      361 ttcatttata ccgctaactc attcataagg aataaatcat gcgattagcc agtcgttttg
      421 gttatgcgaa ccagatacgc cgtgaccgcc ccttaactcg tgaagaactg atgcaggtcg
      481 tccccagcgt attcggggaa gagaaacaca catccagaag cgagaactat acctggattc
      541 ccaccatcac cgtgctggaa agcctgcagc gggaaggctt tcagccattc ttcgcctgcc
      601 agacccgcg
//
LOCUS       DAZKFU020000700          609 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZKFU020000700 DAZKFU020000000
VERSION     DAZKFU020000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.710"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..475)
                     /locus_tag="ACHOQQ_RS26570"
                     /old_locus_tag="pgaptmp_005314"
     CDS             complement(<1..475)
                     /locus_tag="ACHOQQ_RS26570"
                     /old_locus_tag="pgaptmp_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HGU4473412.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHSIKCQNCDSEIWFSGIDIDTQILFQTAEVDAFSSSNGVSIINGVILNDVSWDVVIE
                     STEVNDLNLFVWRFFERYVVSSFFIKGKEFCCESDKYRFNKIIPYLLGYSHEYSAQ"
BASE COUNT          203 a          133 c           92 g          181 t
ORIGIN      
        1 tctgagcaga atactcatga ctataaccca aaagatacgg tattatctta ttaaagcgat
       61 atttgtcaga ttcacaacaa aactccttgc cctttataaa aaagctacta actacgtatc
      121 tttcgaaaaa tctccagaca aacaaattca agtcattcac ttcggtactt tcaatcacca
      181 catcccatga tacatcattc aatattacac cattaataat gctcactcca tttgaggacg
      241 aaaaagcatc aacttcagca gtctgaaata aaatttgcgt atcaatatca ataccagaaa
      301 accatatttc agaatcacaa ttctgacact ttattgagtg taaaaaaaca gagtttgagt
      361 ctatgaattt tcctgactct gtgcggacac agactggata ttttgcggat aaaataattt
      421 ccggcaggcc tgattgacgt atgagtgctc tttctctagt atccatcgca cacataggaa
      481 cctcctcaaa ggattctatg gacagtcgat gcagggagct agtccctccc tgcatcggcg
      541 attttcaagt tcaaatttta acgtggcatt aaccacataa ttaatatact cgttatgtgg
      601 ttaattgtc
//
LOCUS       DAZKFU020000701          603 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZKFU020000701 DAZKFU020000000
VERSION     DAZKFU020000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.711"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..397)
                     /gene="tssL"
                     /locus_tag="ACHOQQ_RS26575"
                     /old_locus_tag="pgaptmp_005315"
     CDS             complement(<1..397)
                     /gene="tssL"
                     /locus_tag="ACHOQQ_RS26575"
                     /old_locus_tag="pgaptmp_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HGU4473413.1"
                     /translation="MRQDIDIDQLMMETWLTVTMLKKGALTPDGTALYNKCVKQVESV
                     REALERAGYDDASVEHITYAQCALLDETVMSRKPEKSGEGGEPDVDEGQAAWRKVPLQ
                     ARFFGSLRAGEALWDRIAEVLRQPSPDPAV"
     gene            complement(394..>603)
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS26580"
                     /old_locus_tag="pgaptmp_005316"
     CDS             complement(394..>603)
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS26580"
                     /old_locus_tag="pgaptmp_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4473414.1"
                     /translation="IYGAALRGIPLIPVSRVPAALPVRMENQYFALDMESPAAHEMLE
                     QGVCMFYVPELLGALELELFAVLRS"
BASE COUNT          140 a          189 c          143 g          131 t
ORIGIN      
        1 aaacggccgg gtccggtgaa ggctgacgca gaacctctgc aatgcgatcc cacagtgctt
       61 caccagcccg aagagaacca aaaaagcggg cctgaagcgg cactttacgc caggccgcct
      121 ggccttcgtc aacgtccggc tccccgcctt ccccgctttt ttcaggctta cggctcatca
      181 ccgtttcgtc caggagggca cattgtgcat aggtaatatg ctccacactg gcatcgtcat
      241 aaccggcacg ctccagcgct tcccgcacac tttcgacctg tttaacgcat ttgttgtaaa
      301 gcgcagtgcc atcgggcgtg agtgcacctt ttttcagcat ggtgacggta agccatgtct
      361 ccatcatcag ctggtcgata tcaatatcct gtctcatgag cgcaataccg caaacagttc
      421 aagttccagg gcaccaagta actcaggcac atagaacata cacactccct gctccagcat
      481 ttcatgtgca gcgggacttt ccatatcaag tgcgaagtac tggttttcca tacgtacagg
      541 caacgcagcc ggaaccctgc tcaccgggat caaagggatc cccctgagtg ccgcgccata
      601 gat
//
LOCUS       DAZKFU020000702          599 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZKFU020000702 DAZKFU020000000
VERSION     DAZKFU020000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.712"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(171..>599)
                     /locus_tag="ACHOQQ_RS26585"
                     /old_locus_tag="pgaptmp_005317"
     CDS             complement(171..>599)
                     /locus_tag="ACHOQQ_RS26585"
                     /old_locus_tag="pgaptmp_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HGU4473415.1"
                     /translation="PVLPDVREFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTA
                     LARQTLAELTGIINAGRYSKVLPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSA
                     RCSDSALVTMHSLACELLPEWREFFDALLENGGKSLWAEN"
BASE COUNT          137 a          174 c          146 g          142 t
ORIGIN      
        1 ccgtaattcg ttttcattcc acatattgtg gaaagttatc cggttcactg aacaacgcca
       61 ttcctccgcc agacgctgat acaaatgctt tttcgcttct gcgcggtcag atgcatcaac
      121 aaacacatgc acttcctcgt gtccgggtgc tgaagccacg gcacgccata tcagttttct
      181 gcccacaggc ttttccctcc gttttccagc agagcatcaa aaaactcacg ccactccggc
      241 aacagttcac aggccagact gtgcatggtg accagcgcag aatcgctgca acgggcagac
      301 actttttcca gacgatgcac cggctgaccg gctgcgtgcc cctgtttata aatattcagg
      361 tcgtaaatct gtgttgaaag aagggtgacc gcaccttcag gcagcacttt gctgtagcga
      421 ccggcgttga taatgcctgt cagctctgcc agtgtctgtc tggccagcgc cgtataatcc
      481 atgcagttaa ccagggtgcg gactttcggc aggggaatac ccagttcacg gtagggcata
      541 aggttttcca tgacattgac cgtgccgcgg atgaactcac gcacatccgg cagaaccgg
//
LOCUS       DAZKFU020000703          596 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZKFU020000703 DAZKFU020000000
VERSION     DAZKFU020000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.713"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(121..399)
                     /locus_tag="ACHOQQ_RS26590"
                     /old_locus_tag="pgaptmp_005318"
     CDS             complement(121..399)
                     /locus_tag="ACHOQQ_RS26590"
                     /old_locus_tag="pgaptmp_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473416.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPEKGAEISVTEE"
BASE COUNT          147 a          169 c          115 g          165 t
ORIGIN      
        1 cgcaggtaca gggcatcgga tatccggtaa atatgttgat ttattaaaga gacagacatg
       61 ccccgtatat aaaattccgt gtcaccgggt gctgaaaaaa actgcaccga cggcgctctt
      121 ttactcctcc gttacggata tttcagcccc cttttcagga gcggtatcct tcacctcgct
      181 gagcccggac tgagccgctt cagaaacctc tgccaccttt ccggcagcct gcattttcac
      241 ttcctccgcg gcatccttca catcactgag ttgagtctgc gcagcttcct ttatatctgc
      301 cgccttctta cttacattct cctttgtcgc atcatcacag cccgcaacca gaaatggtgc
      361 ggaaatacat aacgccagaa ttgttttttc agcatacaat ctctcattat taagtgttaa
      421 cgcagaacag cttatatttc gtccccggga aatggtcaaa agacaaccag aacatcacag
      481 tgtcagggca cactcccggt atatgctttt ataaccataa catgctgttc ctgatattac
      541 cggaaatgtg cccactacat ttaacctgtt ttcagcaccg ggtcatcggc tgtgct
//
LOCUS       DAZKFU020000704          596 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZKFU020000704 DAZKFU020000000
VERSION     DAZKFU020000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.714"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            70..>596
                     /locus_tag="ACHOQQ_RS26595"
                     /old_locus_tag="pgaptmp_005319"
     CDS             70..>596
                     /locus_tag="ACHOQQ_RS26595"
                     /old_locus_tag="pgaptmp_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_453701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HGU4473417.1"
                     /translation="MSMNNTVSSEQKTLDIICLGRVAVDLYGQQIGSRLEDMTTFAKY
                     LGGSSGNVAYGTAIQGLRSAMLARVGDEHMGRFIREELNRVGVNTQCLLTDPERLTAL
                     VILGIKDQDTFPLIFYRDNCADMALAPEDIHETFIASSRALAVTGTHLSHINTRAAVL
                     KALTYAQKHGLRRVL"
BASE COUNT          157 a          147 c          140 g          152 t
ORIGIN      
        1 ctttttgttg aaatcgaaat tatataacct tagtgtaaac aaaaatcaca cggtcttctg
       61 taggaggata tctctatgaa caacacagtc agcagtgagc aaaaaacgct ggacattatc
      121 tgtctggggc gtgtcgccgt tgatttatat ggtcaacaaa tcgggtcacg tctggaagac
      181 atgacaacct ttgcaaaata tttaggaggc tcatcaggca acgtcgctta cggcacagca
      241 attcagggac tacgttccgc catgctggca cgtgtgggcg atgaacatat gggacgtttc
      301 atccgcgaag aattaaaccg tgttggcgtc aatacacaat gcctgttgac tgatcctgaa
      361 cgtctgacag ccctggtgat ccttggtatt aaagatcagg acaccttccc gctgatcttt
      421 tatcgagata actgtgcgga tatggcgctg gcgcctgagg atatccacga aacattcatt
      481 gcctcttcac gtgccctggc cgtcaccggc actcatcttt ctcacatcaa tacccgcgcc
      541 gcagtgctta aagcgctgac ctatgctcaa aaacacggtc tgcgtagagt gctgga
//
LOCUS       DAZKFU020000705          594 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZKFU020000705 DAZKFU020000000
VERSION     DAZKFU020000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.715"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..449
                     /locus_tag="ACHOQQ_RS26600"
                     /old_locus_tag="pgaptmp_005320"
     CDS             87..449
                     /locus_tag="ACHOQQ_RS26600"
                     /old_locus_tag="pgaptmp_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGU4473418.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVDAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
BASE COUNT          173 a          146 c          147 g          128 t
ORIGIN      
        1 aactattgca atgtcatcag ttgtgaacaa agcacctggt cgcgcatact aggggctata
       61 aatttatctt tatcagaagc catcacatga aagaagtcga aaaaaacgaa atcaaacgtc
      121 tcagcgatcg tctggacgcc atccgccacc agcaggccga tctgtcgctg gttgatgccg
      181 cagacaaata tgccgagctg gaaaaagaga aagccacgct ggaagcagaa attgcccgcc
      241 tgcgtgaagt tcacagccag aaactgagta aagaagcaca gaagctgatg aagatgccgt
      301 ttcagcgcgc aattaccaaa aaagagcagg ctgatatggg caagctgaag aaaagtgttc
      361 gcggactggt ggttgttcac ccaatgaccg cgctgggccg cgaaatgggc ctggaagaga
      421 tgactggatt ctctaagact gcgttttaag cccctcagac gattggtacg atcgtatcaa
      481 cccgcatctg gcttgcgtta gctggtcaat gcggggactc ccctgtctgc tcgcctaatt
      541 actgaacaat ccactccctc tacatcaatt taccgctggt tgatattctg aaaa
//
LOCUS       DAZKFU020000706          590 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZKFU020000706 DAZKFU020000000
VERSION     DAZKFU020000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.716"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            97..>590
                     /locus_tag="ACHOQQ_RS26605"
                     /old_locus_tag="pgaptmp_005321"
     CDS             97..>590
                     /locus_tag="ACHOQQ_RS26605"
                     /old_locus_tag="pgaptmp_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4473419.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATS"
BASE COUNT          178 a          107 c          135 g          170 t
ORIGIN      
        1 tatgcttgat ttaaatggcg gacaattatt gttattatta agtctaatgt catttaaagg
       61 cattatcata tttacgaaga gatcaggatg ataacgatga aaaaaagtgt attgacggcg
      121 tttataactg tggtatgtgc aacgtccagt gttatggcag ctgatgataa tgctatcacg
      181 gatggttcag taacatttaa tggtaaagtt attgctccag cttgtaccct ggtcgctgcg
      241 acgaaagatt ccgtggtgac tttgccagat gttagtgcca cgaagttaca aaccaatggt
      301 caggtttctg gcgtgcaaac tgatgtgcca attgaattaa aagattgtga tactaccgta
      361 acaaaaaatg caacgttcac ctttaatggc actgcggata ctactcagat tacagcgttt
      421 gctaaccagg cctcatctga tgctgctaca aacgtggccc tgcaaatgta tatgaatgat
      481 ggtacaacgg ccatcacgcc agacacagaa accgggaata ttttgttgca agatggagat
      541 cagacgttga cttttaaagt tgattatatc gctacgggga aagcgacttc
//
LOCUS       DAZKFU020000707          588 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZKFU020000707 DAZKFU020000000
VERSION     DAZKFU020000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.717"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..379)
                     /locus_tag="ACHOQQ_RS26610"
                     /old_locus_tag="pgaptmp_005322"
     CDS             complement(<1..379)
                     /locus_tag="ACHOQQ_RS26610"
                     /old_locus_tag="pgaptmp_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HGU4473420.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKF"
     gene            complement(403..>588)
                     /locus_tag="ACHOQQ_RS26615"
                     /old_locus_tag="pgaptmp_005323"
     CDS             complement(403..>588)
                     /locus_tag="ACHOQQ_RS26615"
                     /old_locus_tag="pgaptmp_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473421.1"
                     /translation="KVKTAEQEQAVPASPSDLGTTASVGIGDNFERLLGASHSEAEAI
                     MRGMTNAEIDALLEKLG"
BASE COUNT          124 a          164 c          140 g          160 t
ORIGIN      
        1 taaacttcgt gcgctgctga ctcatacgtc cgcctgcatc caccaggtga cgtccctgat
       61 tgattttcag ggagaagtca gcatggctga gatcctcacc acgaccttca acacgctcat
      121 ctcccatcgt cggacgtttt gagagtttgt gcatgatgct gaaggtcact tcatcaccgg
      181 cctgtttgtt aaggtctgtg atacggacaa ccggcgcacc tgcgctggtc tgcttcgtgc
      241 ttttcttgtc cggcgaaacc gcttttggcg cttcctgctg ttcagtaagg atattaacca
      301 tcgagcggtt gcggttggca gcagtaaaaa gcgccacctg atacagctta ttcgcctggg
      361 ctgatgttac agtcgtcatt acttcagtac tccttcagta agttacccga gcttctccag
      421 aagtgcgtct atttcagcat tcgtcatacc acgcataatc gcctcagcct ctgaatgaga
      481 agcaccaagt aaccgttcaa aattatcacc gattccgacg gaggccgtgg tgcctaaatc
      541 tgacggggaa gcaggtactg cctgctcctg ttcagcggtc ttcacttt
//
LOCUS       DAZKFU020000708          587 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZKFU020000708 DAZKFU020000000
VERSION     DAZKFU020000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.718"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..404)
                     /locus_tag="ACHOQQ_RS26620"
                     /old_locus_tag="pgaptmp_005324"
     CDS             complement(<1..404)
                     /locus_tag="ACHOQQ_RS26620"
                     /old_locus_tag="pgaptmp_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGU4473422.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVV"
BASE COUNT          175 a          136 c          125 g          151 t
ORIGIN      
        1 acaacaccca gactcaccaa catacggatg cgaagatcaa taaccatatg ctttgccagc
       61 tgttccgtaa gtgagttggt aattgccact gcatatatca acacgacgac aaaaaaggcg
      121 atgaagtaca gcgtggtaat cagattacca atcttcttgc catagtagtg tgttactgcg
      181 cccgttatcc cttcacctgc tgatgttttc gatgagagga tgaactggca taaggcttta
      241 tgtggccaat atgttaaagg ccaggcgacc agagcagtaa taaacaggac cactgcccct
      301 gccgaaccta actgaatagg aaggaagagc gtgcctgcac caacggctgt gccgtagagc
      361 gcgaaactcc acagagtttc ttcttttgac caaatattag acattatctg aacgtatcat
      421 ctacaaatta aacaaaatag agggcaattt accataaacc aggttaagta tgggagatta
      481 acgccctatc tacccctcta ttggtgggtt agtggttgca aaccttacgt gccagttatg
      541 tctctgactg ctgctcaggc atatttcctt tgtgataaaa atcactt
//
LOCUS       DAZKFU020000709          582 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZKFU020000709 DAZKFU020000000
VERSION     DAZKFU020000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.719"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..299)
                     /gene="cspH"
                     /locus_tag="ACHOQQ_RS26625"
                     /old_locus_tag="pgaptmp_005325"
     CDS             complement(87..299)
                     /gene="cspH"
                     /locus_tag="ACHOQQ_RS26625"
                     /old_locus_tag="pgaptmp_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGU4473423.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT          180 a          119 c          119 g          164 t
ORIGIN      
        1 gaaaaagtaa cttgtgttat aatacatggt gttgactatt taactatgcc aataaaacca
       61 gtcagcttta aacaagcagc gtcatattaa gagagataaa catttgccgc tgttggtcct
      121 cgcaggccat ttacgcggca aaattccacg cgtaatcctg gtataagcac ttctgcgtcg
      181 cggggagtga atgcggaaat atggacctga acttctttac gaccgtcgga ggggataatg
      241 aatcctttgc cgcttttgcg atcaaaggtt ttgacaattc ctgtcatttt acgggacaaa
      301 caaattcctt actgaaaata ctgcgctgca ctatacgggg ttaataaaat aaagccagcg
      361 atatttaaga ccgccggacg gctaaaataa aatttgctta atctcaatta tcatgcgtta
      421 atagctgcgt cggtttgaaa gacagacagc atacaaagta gtttactaaa gcagttctca
      481 ttatcaggca ttatcccctt cttttgagtc tctctcctga acactaagta gtttctgtat
      541 taaagccctg tttgccgaaa ggcccaaaat gaaggaagta aa
//
LOCUS       DAZKFU020000710          580 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZKFU020000710 DAZKFU020000000
VERSION     DAZKFU020000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.720"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..534
                     /locus_tag="ACHOQQ_RS26630"
                     /old_locus_tag="pgaptmp_005326"
     CDS             <1..534
                     /locus_tag="ACHOQQ_RS26630"
                     /old_locus_tag="pgaptmp_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HGU4473424.1"
                     /translation="HWVAEPSASQGMVVSPDFLRAINQLAELSDIVFSQGDAGVHFEL
                     MARPSRDVARVQLTLDEQNLDYFNQMESWQSFTWPGNTYYPGVSLSWRSVSSGMQLYA
                     SHQGNWGFIRLLDEALITPLDASRTQLVWITADGNPLKFVMRSELGDGPLALLKLQGF
                     TLPTAIFNVAAGTEGVE"
BASE COUNT          121 a          151 c          171 g          137 t
ORIGIN      
        1 cactgggtcg ctgagccttc cgcaagccag gggatggttg tcagtccgga tttcctccgg
       61 gcgattaatc agctggcaga gctgtcagat atcgtgttct ctcagggcga tgcgggggtt
      121 cacttcgaac tgatggcgcg tccttcacgg gatgttgcca gagtgcagct tacccttgat
      181 gaacagaatc tggattactt caatcagatg gaaagctggc agagcttcac ctggccgggg
      241 aatacttact atccaggtgt gtcgctcagc tggcggagtg taagcagtgg tatgcaactc
      301 tacgccagtc accaggggaa ctgggggttt attcgcctgc tggacgaagc cctgatcacg
      361 ccacttgatg ccagccgcac acaactggtg tggatcactg ccgatggtaa tcctctgaaa
      421 ttcgtcatgc gcagcgagct gggcgacggt ccgcttgcct tactgaagct acaggggttt
      481 acgctgccca ccgccatttt taacgttgcc gccggcacgg aaggtgttga gtaaaaatga
      541 acaggggcat ttatccgacg ccggccttta ctgaagatct
//
LOCUS       DAZKFU020000711          580 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZKFU020000711 DAZKFU020000000
VERSION     DAZKFU020000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.721"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>580)
                     /locus_tag="ACHOQQ_RS26635"
                     /old_locus_tag="pgaptmp_005327"
     CDS             complement(<1..>580)
                     /locus_tag="ACHOQQ_RS26635"
                     /old_locus_tag="pgaptmp_005327"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HGU4473425.1"
                     /translation="RERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQVIVWL
                     EKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKREKTPPP
                     LCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHEESSEV
                     LEAVGRSDPSVLPQLAELESGRVSRIWNDKKIT"
BASE COUNT          143 a          187 c          129 g          121 t
ORIGIN      
        1 cgtaattttt ttatcattcc agatacggga tacccgtcct gattccagtt cagccagttg
       61 tggaagcacg gacggatctg aacgcccgac agcctcaagc acttcagaac tctcctcatg
      121 catcgaggca ggcagataac cacagtctgt cctgggataa gttgttgctt tgtgagtctc
      181 ataaagggac tgtgcgatat ccagaacggt ctgagccccc atgccaaact tccgggaaca
      241 ggcttcctgc aacgttccca gcgaaaaaca caaaggtggc ggtgttttct ccctcttctg
      301 tacaacatcc tgtacaacag cagaggactg ctgacggcac agaccgagaa cagcaccggc
      361 cgcctgcaca ttcacacaac gtccctcatc atcgcagtac tgctcagctg ccacccagtt
      421 actgcggaaa cggagcccct ccttttccag ccagacaatc acctgccacc aggaaaccgg
      481 cacaaatgtg gcgatctcct gatcccgtct gaccaccatt gccagcgtgg gtgtctgcac
      541 ccggcccact gacaaaaccc cagaaattcc acgttcccgt
//
LOCUS       DAZKFU020000712          577 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZKFU020000712 DAZKFU020000000
VERSION     DAZKFU020000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.722"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..172
                     /locus_tag="ACHOQQ_RS26640"
                     /old_locus_tag="pgaptmp_005328"
     CDS             <1..172
                     /locus_tag="ACHOQQ_RS26640"
                     /old_locus_tag="pgaptmp_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colicin V secretion protein CvaA"
                     /protein_id="HGU4473426.1"
                     /translation="IAMPEKQIIRYDEKYLPLENGMKAESTLFLEKRRIYQWMLSPFY
                     DMKHSATGPLND"
     gene            165..>577
                     /locus_tag="ACHOQQ_RS26645"
                     /old_locus_tag="pgaptmp_005329"
     CDS             165..>577
                     /locus_tag="ACHOQQ_RS26645"
                     /old_locus_tag="pgaptmp_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGU4473427.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRY"
BASE COUNT          156 a          126 c          148 g          147 t
ORIGIN      
        1 cattgcgatg cctgaaaagc agattatcag atatgacgaa aaatacctcc ctctggaaaa
       61 tggaatgaaa gccgaaagta cactatttct ggaaaaaagg cgtatttacc agtggatgct
      121 ttctcctttc tatgacatga aacacagtgc aacaggaccg ctcaatgact aacgggaatt
      181 tcagacaaat tataaatcag cttgatatgc gctggcgacg tcgtgttccg gttattcatc
      241 agacggagac cgctgaatgt ggactggcct gcctggcaat gatatgcggt cattttggta
      301 agaatattga cctgatatct cttcgccgga agtttaatct ctcggcccgt ggagcaaacc
      361 ttgcaggaat caatggaata gcagagcagc tggggatggt cacccgggct ctttcactgg
      421 agctggatga acttggtgcc ctcaaaatgc cgtgtattct ccactgggat ttcagtcact
      481 tcgtcgtgct ggtcagcgta aagcgtaacc gttatgtact gcatgatccg gccagaggca
      541 gaagatatct cggtcgggag gaaatgagcc ggtattt
//
LOCUS       DAZKFU020000713          577 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZKFU020000713 DAZKFU020000000
VERSION     DAZKFU020000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.723"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>577)
                     /locus_tag="ACHOQQ_RS26650"
                     /old_locus_tag="pgaptmp_005330"
     CDS             complement(<1..>577)
                     /locus_tag="ACHOQQ_RS26650"
                     /old_locus_tag="pgaptmp_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4473428.1"
                     /translation="SYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGS
                     YHSNGLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMKSKSVDTRSIYRE
                     LGATLSYNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGI
                     KASFSSTLSGHLGVGYSNGADVESPWNAVAGV"
BASE COUNT          133 a          181 c          116 g          147 t
ORIGIN      
        1 tcacaccagc caccgcgttc cacggggatt ccacatcggc accgttgcta taccccaccc
       61 cgagatgccc gcttaacgta ctgctgaatg aggctttaat acctgcctgg tatattccac
      121 gtctgcccga caaatcattg acgaaattac cgtcattatt caccttcacc cggttatcat
      181 cgacaaattc tttgcgcaca gccgccttca gccacggctc aacttccata ccgttcccca
      241 gacgcatgtt gtaactcagc gttgccccca gttcacgata tatactgcgg gtatcgactg
      301 atttcgattt catgccattg gataaatgat attcggggtt atcagcggtg aaccccgtta
      361 acgatgcata cggcgtcagg ttccagttac catcggtaaa tcgcatcccg gtttcaatgt
      421 gaccgcccag cccgttgctg tggtaactgc cattggcggc tccaccgctg ctcattttac
      481 cggctacgtt actttcaaaa cggttcagct tcacgacacc gtccagatag aaaccacttt
      541 catgttccca actggcatag ccgcccagag aataact
//
LOCUS       DAZKFU020000714          575 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZKFU020000714 DAZKFU020000000
VERSION     DAZKFU020000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.724"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..323
                     /locus_tag="ACHOQQ_RS26655"
                     /old_locus_tag="pgaptmp_005331"
     CDS             <1..323
                     /locus_tag="ACHOQQ_RS26655"
                     /old_locus_tag="pgaptmp_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4473429.1"
                     /translation="TQMGYYHYISRLIPFGHPDEPYAPIFTDSVVCIMNLVLYDDYIT
                     VLSRARLQEARFKEILCSIPVKYQLPVSQYGLIYPRKRLMTAPAQIMIEYFQQVIQRY
                     DWNG"
BASE COUNT          178 a          123 c          116 g          158 t
ORIGIN      
        1 aaactcaaat gggatactat cattacatat cccgtcttat tccctttgga catccggatg
       61 aaccatatgc tccaatattt accgattcgg tagtttgtat tatgaatctt gttctctatg
      121 atgactatat aacggttctc tccagagcaa ggttacagga agcaagattc aaagaaatat
      181 tgtgttcaat tcctgttaag tatcaactac ccgttagcca gtacgggctt atttatccac
      241 gaaaaaggct aatgacggca ccagcacaga taatgattga atattttcaa caagtgatac
      301 aacgatatga ctggaatgga tgagcaatac ccggaatgat ggtgtacacc gggataacaa
      361 tttattcgga tgtgccaatc ccacggttgt aatgggcaat ataattgtta gccgattcgc
      421 taatggcagg gaaaagcacg gcataaatat aagcccacac acatgtaacg tactgcagat
      481 actcgcttgg cagagttgtt acttcttggg aggcagtacc agccatcaga tattcccgtt
      541 ccatgccaaa acctgagtta cccggatacc attac
//
LOCUS       DAZKFU020000715          574 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZKFU020000715 DAZKFU020000000
VERSION     DAZKFU020000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.725"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..>574
                     /locus_tag="ACHOQQ_RS26660"
                     /old_locus_tag="pgaptmp_005332"
     CDS             23..>574
                     /locus_tag="ACHOQQ_RS26660"
                     /old_locus_tag="pgaptmp_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4473430.1"
                     /translation="MKYMPKLQQIKFFNEIVLCGSLRAAARKMNISQPALSRTIKELE
                     NVLGCNLLIRTRDGIVLTEAGKIFSVHSRMILEEIETAALEVAKVNIDNTGKVAFGIS
                     SLFGVTILNNVLDNFRMKYPKKRVMIREGQLSSLIPFLREGVLDFAMGTLTKDMPVGD
                     FIVVELFNAPFTIVARKHHPLRNC"
BASE COUNT          195 a           92 c          123 g          164 t
ORIGIN      
        1 cagaaaatta tcaggcgaaa aaatgaaata tatgcctaaa cttcaacaaa ttaaattttt
       61 caatgaaata gtcctgtgcg gaagtttaag agctgctgcc cgtaaaatga atatatctca
      121 gccagcatta agcagaacga taaaagaact ggagaatgta ttaggatgta accttctgat
      181 tcgaacaagg gatggtatcg ttttaacaga agctggaaag atattctcgg tccattcacg
      241 tatgattctt gaagagattg aaactgcagc tcttgaagtt gcgaaagtaa atatagataa
      301 cacaggaaaa gtagcttttg gaatttcttc gctgtttggc gtaacaatac tgaataatgt
      361 acttgataac ttccggatga aatatccgaa aaagcgtgtg atgataagag agggacagtt
      421 gtcctccctt atccctttcc tgagagaagg tgttctggat tttgcaatgg gtacattaac
      481 aaaagacatg ccagttggag attttattgt ggttgaattg tttaacgcac cgttcactat
      541 agtggcaaga aaacaccatc ctttaaggaa ctgt
//
LOCUS       DAZKFU020000716          573 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZKFU020000716 DAZKFU020000000
VERSION     DAZKFU020000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.726"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            167..>573
                     /locus_tag="ACHOQQ_RS26665"
                     /old_locus_tag="pgaptmp_005333"
     CDS             167..>573
                     /locus_tag="ACHOQQ_RS26665"
                     /old_locus_tag="pgaptmp_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473431.1"
                     /translation="MGCNKKLNKFLLFVSCMLLSFVTYSQDSSNPQFSDYLVPVSNGP
                     FEKNIHFNKEQENYSQHWKNAVQEELKKPVNFAGHFRIYTAFGGHGKECLRDNWVCGW
                     VIDKLSGEVVATLPSDNNGSYNYADVSDNGSPVG"
BASE COUNT          185 a           90 c          131 g          167 t
ORIGIN      
        1 atctgcggta attgatgccc caagagaacg aaaaatggta aaaagaaata caggttagta
       61 cctagggtgg cagaagttga gggggagtcg tactattctg gtgatggatt gaataaagct
      121 cacctattac cagatacgat gattaaagct cttgaggatt ctaaaaatgg gatgcaataa
      181 aaaattaaac aaatttttgc tttttgtgtc atgcatgctc ttgtcgtttg taacgtactc
      241 acaagacagt tctaatcctc agttttctga ctacctggtt cctgtaagca atggaccatt
      301 tgaaaagaat attcacttta ataaagagca ggaaaattac tcgcaacact ggaagaatgc
      361 agtccaagag gaactaaaaa agcctgttaa ttttgcaggg cactttagaa tctatacagc
      421 ctttggcggt catggtaaag aatgccttcg tgataattgg gtatgtggtt gggttattga
      481 taaactatca ggggaagttg tagcaacact tccatcagat aataatggaa gttataacta
      541 tgctgatgtg tctgataatg gtagccctgt tgg
//
LOCUS       DAZKFU020000717          570 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZKFU020000717 DAZKFU020000000
VERSION     DAZKFU020000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.727"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..509
                     /locus_tag="ACHOQQ_RS26670"
                     /old_locus_tag="pgaptmp_005334"
     CDS             42..509
                     /locus_tag="ACHOQQ_RS26670"
                     /old_locus_tag="pgaptmp_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473432.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGVLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
BASE COUNT          173 a          112 c          155 g          130 t
ORIGIN      
        1 gaatgagcac tactgtcatt cacatcaaag gggaatgaga tatggctatt gcagagagaa
       61 cgggtatctc ctcgcgcggc gatacttacg aaatctggtt gggagcaaac tgttatcact
      121 accgctctga tggttatgca gtgcttctga aagaaggcag gaatcgtgct tatccatgtc
      181 gggtgacgag tgaaacgatt aaatctgcgc tggcagcaat tgttgaattg caacagaaat
      241 taactgcggc agagaagaaa aaggagtgtg agaaccacgg ggttcttttg ccagctgaga
      301 tgttaattcg ctgtctggaa gatcaaacag ggggacggtg gatccgtgca catgaaccag
      361 taatgctgat gaagcgatta atcagtaacc tgaaaaaagc cagaccagaa tgtaccgttc
      421 ctgatgaagc gctggcatat ctccggcgta actgtctggt gaatatgggc gatatacccg
      481 ttaccagcag acggatgtct gacgtataac aaaaaggaaa agtgtaatga cattacccgt
      541 agacagcctg attgcgtaca cgctggaaaa
//
LOCUS       DAZKFU020000718          564 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZKFU020000718 DAZKFU020000000
VERSION     DAZKFU020000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.728"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..183
                     /locus_tag="ACHOQQ_RS26675"
                     /old_locus_tag="pgaptmp_005335"
     CDS             <1..183
                     /locus_tag="ACHOQQ_RS26675"
                     /old_locus_tag="pgaptmp_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HGU4473433.1"
                     /translation="TVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRAL
                     QRSVLPDDDEGDENDE"
     gene            176..>564
                     /locus_tag="ACHOQQ_RS26680"
                     /old_locus_tag="pgaptmp_005336"
     CDS             176..>564
                     /locus_tag="ACHOQQ_RS26680"
                     /old_locus_tag="pgaptmp_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HGU4473434.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGGRY
                     RRCVTFYGFSERPQVAAYAFDVLCRQL"
BASE COUNT          110 a          140 c          190 g          124 t
ORIGIN      
        1 acagtcctcc ccgtgagtct gaaagacctg cttacagggg gcagtgcacc ggaagcgtgg
       61 ccactgctgg atgacgacca tgccgtgaag ctgctgcgtc ttctgagtgt gctgcgccgc
      121 ctgcgagccc tgcagcgcag tgtattaccc gacgacgatg agggggatga gaatgatgag
      181 tgatatgtcg catcaggaac gtctgatcag gcgggtcagg aaactgctgg agctgagccg
      241 taataacagc aatgcccatg aggccgggct ggcgcttgca agggcgcaac agctgatggc
      301 gaaacacggt atcacggagc aggatgccgg actttcttcg gtgcagacct cttcatcaca
      361 gggggcacct tctgatgctg aaaaagttcc ggcgtggatg tttcgtctgg tcatggtggt
      421 gacacagaca tttggctgcc gggcgtatta cgactggcgg gcggtgcccg gaggccggta
      481 tcgccggtgt gtgacgtttt acggtttcag tgaacgtcct caggtggcgg cttatgcgtt
      541 tgatgttctg tgccgtcagc taag
//
LOCUS       DAZKFU020000719          563 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZKFU020000719 DAZKFU020000000
VERSION     DAZKFU020000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.729"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..107
                     /locus_tag="ACHOQQ_RS26685"
                     /old_locus_tag="pgaptmp_005337"
     CDS             <1..107
                     /locus_tag="ACHOQQ_RS26685"
                     /old_locus_tag="pgaptmp_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HGU4473435.1"
                     /translation="SWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
BASE COUNT          169 a          124 c          147 g          123 t
ORIGIN      
        1 tgtcctggcc gacgtcggtg tatccgaacc tgctgatatg gccggcagcc atgctgctgg
       61 gggtggccat caccgtgacc atggcatcct tcaagaaata tttataatcc acagaggccg
      121 gacaccctga tgcaaaactt gttttgtggc agggtgtcgg cagacaagcc acgcccttac
      181 ggtcagtgcg cgtcagtgtc agagtaacga cagcgccgga atgacaacaa tgttgacgaa
      241 aataatgaac agagtgatgg ccctgatgac gtcatagatc cacggggggc aacgaggttc
      301 ttcataaatg aaaaaataca caacacaaac ccaggtcaga aggaacaggg cccagaacgg
      361 aagggcagta cggagaaatt ccatgctgat gtgtttcctc tttaaattgt taatgcggtg
      421 ccattaacgg gaaacagtag tgatgccgtg ttttaataaa caggaataaa tcattcaggc
      481 tggaataaat ttatttgaac agaatctgga acagcgagat gcagaaactg gctatgctga
      541 cagcccccag aacatagttt att
//
LOCUS       DAZKFU020000720          561 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZKFU020000720 DAZKFU020000000
VERSION     DAZKFU020000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.730"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>561
                     /locus_tag="ACHOQQ_RS26690"
                     /old_locus_tag="pgaptmp_005338"
     CDS             <1..>561
                     /locus_tag="ACHOQQ_RS26690"
                     /old_locus_tag="pgaptmp_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HGU4473436.1"
                     /translation="LKKQFNFKLIEHEWENIQHIICSLSRKTTIQSTVVKKLSNTKRS
                     NKTLAALREYDRVIKCIYVLDYADNKTLRQFVQQALNRGEAYHQLRRAIASVNGNQFR
                     GGNDYQIDQWNDCARLIANCVIYYNSAILSRLVEKFEKENNKKAIDVLANLSPVAWRH
                     ILLGGNYSFEDQVAITNLDSLLEGVDP"
BASE COUNT          209 a           91 c          110 g          151 t
ORIGIN      
        1 acttaaaaaa caatttaact ttaagctcat tgaacatgaa tgggaaaaca tccaacatat
       61 tatttgttct ttaagcagaa aaacaaccat tcaaagtacg gttgttaaga agttatctaa
      121 tacaaagcgt agcaacaaga cattagcagc acttcgggag tatgaccgag ttataaaatg
      181 catatatgtc ctggattatg ctgacaataa aaccttaagg caatttgtgc aacaagcact
      241 gaacagaggt gaagcttatc atcaactaag aagagcaata gcatcagtaa atggaaatca
      301 atttcgcggt ggtaatgact accaaataga tcaatggaac gactgtgcga ggttaatagc
      361 taattgtgta atctattata attcagcaat attatccagg ctggtcgaga aatttgaaaa
      421 agaaaataat aaaaaagcga tagatgttct ggctaactta tccccagtag catggagaca
      481 cattctcctt ggagggaatt attcttttga agatcaagtt gcgatcacaa atttggacag
      541 tcttttggag ggagtagacc c
//
LOCUS       DAZKFU020000721          561 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.731, whole genome shotgun sequence.
ACCESSION   DAZKFU020000721 DAZKFU020000000
VERSION     DAZKFU020000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.731"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>561)
                     /locus_tag="ACHOQQ_RS26695"
                     /old_locus_tag="pgaptmp_005339"
     CDS             complement(<1..>561)
                     /locus_tag="ACHOQQ_RS26695"
                     /old_locus_tag="pgaptmp_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HGU4473437.1"
                     /translation="GICYWLFCTKFSCKVRTSVKVSHYIPEVVVSTYTAPGGNPWREM
                     SAVSSTAGGPENAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIG
                     GMVPGYSCESPVTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWG
                     NVYPRSGFVTQTDDDKAAAVVAQRVA"
BASE COUNT          124 a          167 c          140 g          130 t
ORIGIN      
        1 tcagccaccc gctgggccac caccgcagca gctttatcat catctgtctg ggtcacgaac
       61 ccggagcggg gatacacatt gccccacata ttctcttctt cctggctgcc tatttcgcgt
      121 ttaccgggga tcagggcctc cgggtacagt gactccggta gtcctgtacg ccaggcaata
      181 ctgtcaagcg tactgagaaa ataaggaaca aaaggtgtca ccggtgactc acaggaatat
      241 cccggcacca tccctccgat aagcgtggtg gccgggtgcc ctgttgcatc ggcatattta
      301 aaacggattg acgaacgacg ttccccctgt accttcatgt ctgcggggtt ccctcctcct
      361 gctgacagac cggcaatacc tttgcttatg gcattctccg gaccaccggc tgtactgctg
      421 accgccgaca tttcacgcca gggatttccg ccaggtgcgg tataggtgga aacaaccact
      481 tccgggatat aatgggaaac tttcactgat gttctgacct tgcaactgaa tttggtgcag
      541 aacagccagt agcaaatccc g
//
LOCUS       DAZKFU020000722          558 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZKFU020000722 DAZKFU020000000
VERSION     DAZKFU020000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.732"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..176
                     /locus_tag="ACHOQQ_RS26700"
                     /old_locus_tag="pgaptmp_005340"
     CDS             <1..176
                     /locus_tag="ACHOQQ_RS26700"
                     /old_locus_tag="pgaptmp_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473438.1"
                     /translation="GAECWYDVVDNGTTDLRQGVVRIRYKYTPVPPLEDLELHQTFTD
                     EFFAGAFASLGGE"
     gene            176..>558
                     /locus_tag="ACHOQQ_RS26705"
                     /old_locus_tag="pgaptmp_005341"
     CDS             176..>558
                     /locus_tag="ACHOQQ_RS26705"
                     /old_locus_tag="pgaptmp_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HGU4473439.1"
                     /translation="MAVPNKLRVFTVYIDGENKLGRVTSFTPPKLTRKTESFRGAGMP
                     GSVSVDFGLDDGALDLSFAVGGADAHLFSRYAGSLDEVRLRFSGEYYSDADIRYVDIE
                     VRGRITEIDMGEAKQGEDTEHSYSM"
BASE COUNT          116 a          130 c          172 g          140 t
ORIGIN      
        1 tcggggcaga gtgctggtat gacgtggtgg ataacggcac aacggatctg cgtcaggggg
       61 ttgtgcgcat tcgctacaag tatacgccgg tgccaccgct tgaagatctt gagcttcacc
      121 agacgtttac cgatgaattc tttgcgggtg catttgcatc actgggaggt gagtaatggc
      181 ggtaccaaac aaacttcgcg ttttcacggt gtacatcgat ggcgaaaaca agctggggcg
      241 ggtgacgtcc ttcacgccgc ctaaactgac ccgaaaaacg gagtccttcc gtggtgccgg
      301 gatgcccggc agtgtttccg tcgactttgg tcttgatgat ggtgcactgg atctctcttt
      361 tgcggtaggc ggcgcagatg cccacctttt ctcccgttat gccgggtcac ttgatgaagt
      421 ccgtcttcgc ttttccggtg agtactattc ggatgcggat atcagatatg tggatattga
      481 ggtccgcggg cgcatcactg aaatcgatat gggggaggcg aagcagggtg aagacactga
      541 gcacagttat tcgatgaa
//
LOCUS       DAZKFU020000723          557 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZKFU020000723 DAZKFU020000000
VERSION     DAZKFU020000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.733"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(243..>557)
                     /locus_tag="ACHOQQ_RS26710"
                     /old_locus_tag="pgaptmp_005342"
     CDS             complement(243..>557)
                     /locus_tag="ACHOQQ_RS26710"
                     /old_locus_tag="pgaptmp_005342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473440.1"
                     /translation="QLILSIYHVICSVTSKINIKNSAKFNIRSTSRLTYDIISQLHLP
                     QNGYFMERSNFLVAIIMLILRSLAIIPGWFVSLGHEIVTFLFIMEIWGKAGNDFFIYI
                     RW"
BASE COUNT          165 a           92 c          125 g          175 t
ORIGIN      
        1 gcaagctaac tcagtgggcg tggtgaaaat aagtgtttac ccgccatcag gctgagcata
       61 attctcatca tgaaatatgt ttcctggttt gtggcttgta actggtcact tctgaagtcg
      121 atctggagag gcttgttgat gttggtgttt tcaggatgat gtttcagata gtttgtttgc
      181 cgtatcgccc ggcgaatggc tgtgattgag gaaggtttaa gtcgtagtga ccaaagctat
      241 atttaccaac gaatgtagat gaaaaaatcg tttcctgctt tcccccatat ctccatgata
      301 aaaaggaatg taacaatctc atggcctaaa ctgacgaacc agccaggaat aatcgctagg
      361 gacctaagaa ttagcatgat aatagccact aagaaattac tgcgttccat gaaatagcca
      421 ttttgtggca aatggagttg actaataatg tcatatgtga gacggctagt tgaacgaata
      481 ttaaattttg ctgaattttt tatgttgatt ttacttgtta cagaacatat cacatgatat
      541 atagataaga ttagttg
//
LOCUS       DAZKFU020000724          553 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.734, whole genome shotgun sequence.
ACCESSION   DAZKFU020000724 DAZKFU020000000
VERSION     DAZKFU020000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.734"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>553)
                     /locus_tag="ACHOQQ_RS26715"
                     /old_locus_tag="pgaptmp_005343"
     CDS             complement(<1..>553)
                     /locus_tag="ACHOQQ_RS26715"
                     /old_locus_tag="pgaptmp_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4473441.1"
                     /translation="NKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFSAGGHIASTFV
                     NQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPGSRKQLLGDNSTSENIIQ
                     YSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSV
                     QWATDLPVGGWIKLFMDWMYSHGF"
BASE COUNT          183 a          123 c           93 g          154 t
ORIGIN      
        1 aaatccatga gaatacatcc agtccataaa cagttttatc catcctccaa caggaagatc
       61 tgtggcccac tgaacactaa agccatgccc tcctttctca tagagatgca tttcagcaga
      121 aacgcctttc tctattaggc cacggtacat gagcagactg ttttcaattg acacaagatc
      181 atcatcacaa caatggagta aaaacacagg aggaaaacat gatgtgacat taagatccgc
      241 actatattgt attatatttt ctgaggtact attgtccccc agtaattgct tcctggagcc
      301 tggatgtgtc acatcatctt tcattgatat tacaggataa attaacccga caaaatcggg
      361 aatagcgaaa atctcttgtt ctttactgtc agaagaacgt ttgaaatcac aaatctgatt
      421 cacaaacgtt gaggcgatat gccccccagc agaaaatcct aatacaccaa tacgatgacg
      481 ctgtagatgc catttatcag cattcttacg aatataaagc attgcagata ttgcatcaat
      541 aaaagttttg ttt
//
LOCUS       DAZKFU020000725          552 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.735, whole genome shotgun sequence.
ACCESSION   DAZKFU020000725 DAZKFU020000000
VERSION     DAZKFU020000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.735"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>552)
                     /locus_tag="ACHOQQ_RS26720"
                     /old_locus_tag="pgaptmp_005344"
     CDS             complement(<1..>552)
                     /locus_tag="ACHOQQ_RS26720"
                     /old_locus_tag="pgaptmp_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU4473442.1"
                     /translation="EGWEDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVYCISPAK
                     FHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQI
                     RTDEKSNEITAIPGLLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGR
                     LNKAFEEKFPLKELNNPEHDSYA"
BASE COUNT          145 a          127 c           98 g          182 t
ORIGIN      
        1 attgcgtaac tgtcatgctc tggattattt aattctttca gtggaaattt ttcctcaaag
       61 gctttattaa gccgcccctg atttcctttt acagcgaata aataatcacc gccctgtttt
      121 tgtatcttct ctgcgatatc tttctggcaa cccatagcat cagttgtgat aatttttcct
      181 ttaatatcca gcatgttaag aagtccaggg atagctgtaa tctcattgga tttctcatcc
      241 gtcctgatct gtccgatgac cagactgtgc attgttgaga acgcactaat gacatgaatc
      301 gctcccttgc ggcgactctt gtcataagaa tgccggagtg tttttccatc aattgcgatg
      361 acgtctttat catctgaaga atggcagtca cgcatccagt taataaagca ctcgtgaaat
      421 tttgcgggac tgatacagta tacaactctg gcaatggtat cgtgaacagg aatgccattt
      481 tcaaaatcac catattgctt caaaaaatcg agatgtgttt ccccaaaatc ctctatatct
      541 tcccaacctt ct
//
LOCUS       DAZKFU020000726          552 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.736, whole genome shotgun sequence.
ACCESSION   DAZKFU020000726 DAZKFU020000000
VERSION     DAZKFU020000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.736"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..503)
                     /locus_tag="ACHOQQ_RS26725"
                     /old_locus_tag="pgaptmp_005345"
     CDS             complement(<1..503)
                     /locus_tag="ACHOQQ_RS26725"
                     /old_locus_tag="pgaptmp_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
                     /protein_id="HGU4473443.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAK"
BASE COUNT          212 a          115 c           98 g          127 t
ORIGIN      
        1 tttttggcat ctttataagc aaattgacaa gcggaaaata aacttcccga aatgacaatc
       61 agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt tattattaca
      121 gccaaaaaaa gaagggataa aaaggcaaaa gcacatagat aagggctata cattcccagc
      181 attttggcaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc atcattgaaa
      241 gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc agtcagtaac
      301 acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt aacaaccagg
      361 atggtcagaa cgaagaacag caatcgctga accatatgcg ctccggaaga ggcgactaag
      421 ttcgtaatac caaaaacaat aacactaaga tacaacaacc aatgagattt aaaacttcta
      481 gcaaatttgt tgaatactgt catccagaaa tccaagttca caaccaatat aaacaattat
      541 caacaatgtg ca
//
LOCUS       DAZKFU020000727          551 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.737, whole genome shotgun sequence.
ACCESSION   DAZKFU020000727 DAZKFU020000000
VERSION     DAZKFU020000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.737"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>551)
                     /locus_tag="ACHOQQ_RS26730"
                     /old_locus_tag="pgaptmp_005346"
     CDS             complement(<1..>551)
                     /locus_tag="ACHOQQ_RS26730"
                     /old_locus_tag="pgaptmp_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473444.1"
                     /translation="QLLNELDEQAKRQAVMNELKAKPRSELLEEYRRLSQKEGRTETE
                     EQQFQAIREVIRPQQEVTPEAQSQPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASR
                     PAERIEKVRPDNRYFTDEKSAMGSDVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSE
                     DVATETINTLADGERIADADPME"
BASE COUNT           96 a          168 c          128 g          159 t
ORIGIN      
        1 tgctccatcg ggtcagcatc agcaatacgc tctccgtcag ccagggtgtt aatggtttcc
       61 gttgccacat cttcagagaa cgcaggatta tccatttcaa cggcatactg attctcgcct
      121 ttcttcacta cggacggttt caggccggtg gcggcggcat tacggaaaac atcactcccc
      181 atggcgcttt tctcatccgt gaaataacgg ttgtccgggc gcaccttttc aatgcgttcc
      241 gctggtctgg acgcaccatt cccgttaatt tcaatgcgga cttcattcgg gtcccggaac
      301 cgcaccgtcg ggtaaatgct cccgttacca tcctccgcat tttcaggctg tgactgtgct
      361 tccggcgtca cttcctgttg tgggcgaatg acttctcgta ttgcctgaaa ctgttgttct
      421 tcagtctcgg tgcgcccctc tttctggctg agtctgcggt attcctcaag cagttcagaa
      481 cgcggcttcg ccttcagctc attcatcaca gcctgtcgct tcgcctgttc gtccagttca
      541 ttcaacaact g
//
LOCUS       DAZKFU020000728          547 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.738, whole genome shotgun sequence.
ACCESSION   DAZKFU020000728 DAZKFU020000000
VERSION     DAZKFU020000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.738"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>547
                     /gene="eilA"
                     /locus_tag="ACHOQQ_RS26735"
                     /old_locus_tag="pgaptmp_005347"
     CDS             <1..>547
                     /gene="eilA"
                     /locus_tag="ACHOQQ_RS26735"
                     /old_locus_tag="pgaptmp_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HGU4473445.1"
                     /translation="IILLLNADGEIVSKEEIIDKVWSASVASDESLTRCIYALRKLLH
                     ENKQCKYIETVYGRGYRFTVPIVIVTDNEPVKSTTTLAVFPFRTEGSINVLKLHYELV
                     QGLSKYAFCGLDILPASVTNEASDFSTIHQFINKTGPEYYIMGQVVHYGKNWRLFIEL
                     IHARTHKLIDHQSIDFNPDNPL"
BASE COUNT          157 a          105 c          106 g          179 t
ORIGIN      
        1 tatcattcta ttacttaatg ctgacggtga aattgttagt aaagaagaga ttatcgataa
       61 ggtctggagc gcgagtgttg ccagtgatga atctttaaca cggtgtattt atgcattgcg
      121 taaacttttg catgagaata aacagtgtaa gtatatcgaa acggtctatg gtcgtggata
      181 ccgttttact gtccccattg tgattgtaac cgataatgaa ccggtaaagt cgaccaccac
      241 tcttgccgtt tttccctttc gtactgaagg ttcgatcaat gttctaaaac tccattacga
      301 actggtccag ggcctttcaa aatatgcttt ttgtggttta gatattttac ccgcttcggt
      361 aacgaatgaa gccagtgatt tctccactat ccatcagttt atcaataaaa caggcccaga
      421 atattatatt atgggtcagg tcgttcacta tggtaaaaac tggcgtttat ttattgaatt
      481 aatccatgcc agaacgcata aattgattga tcatcaaagt attgatttca accccgacaa
      541 tcctctt
//
LOCUS       DAZKFU020000729          546 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.739, whole genome shotgun sequence.
ACCESSION   DAZKFU020000729 DAZKFU020000000
VERSION     DAZKFU020000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.739"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>546)
                     /gene="lpfA2"
                     /locus_tag="ACHOQQ_RS26740"
                     /old_locus_tag="pgaptmp_005348"
     CDS             complement(<1..>546)
                     /gene="lpfA2"
                     /locus_tag="ACHOQQ_RS26740"
                     /old_locus_tag="pgaptmp_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HGU4473446.1"
                     /translation="LALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPETKDQVVDLG
                     KVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNLAIGTPLDNS
                     NDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSASTPISNGNAS
                     MKFMARYIATETTIDPGTANA"
BASE COUNT          151 a          133 c          123 g          139 t
ORIGIN      
        1 cggcgttagc tgtaccaggg tcaatagtcg tttccgtagc aatataacga gccatgaact
       61 tcatggatgc attaccatta gaaatcggag ttgatgcaga attttcatat aacttgacct
      121 gagtgttatc tgcacggtta tataaacgaa tagctacgcc tttcgctgca ctaacggcac
      181 ccgcaccagt ataatcccca ctgttatcac tcgggctaat accagcggca gcatcgttag
      241 agttatcaag cggggtaccg attgccaggt tgccattacc atgagcatct tcattaccat
      301 cgaaacgaat tgcggcggac ttaaaggttt ccgggcattg agtcagatcg atagaaaaag
      361 ccgtcggggc agccacatca tcgacgccac taaaggctgt acggtttact ttgcctaagt
      421 caacgacctg atctttagtt tcaggagtga cttcgcaagt actgtcaata atttcaccac
      481 ggaaatggac tgttccatca gccgcctgta ggtgacctgc cataaacaca gaagttaacg
      541 ctaatg
//
LOCUS       DAZKFU020000730          545 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.740, whole genome shotgun sequence.
ACCESSION   DAZKFU020000730 DAZKFU020000000
VERSION     DAZKFU020000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.740"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>545
                     /gene="waaH"
                     /locus_tag="ACHOQQ_RS26745"
                     /old_locus_tag="pgaptmp_005349"
     CDS             <1..>545
                     /gene="waaH"
                     /locus_tag="ACHOQQ_RS26745"
                     /old_locus_tag="pgaptmp_005349"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGU4473447.1"
                     /translation="DFRTCMESLITQTWTALEIIIINDGSTDNSVEIAKHYAENYPHV
                     RLLHQANAGASVARNHGIEVATGKYVAFVDADDEVYPTMYETLMTMALEDDLDVAQCN
                     ADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSRRWTHVVWMGVYRRDVIVKN
                     NIKFIAGLHHQDIVWTTEFMF"
BASE COUNT          136 a          120 c          140 g          149 t
ORIGIN      
        1 gatttccgca cttgtatgga atctttaatt acgcaaacct ggactgctct ggaaatcatt
       61 attattaacg atggttcaac ggataattct gttgaaatag caaagcatta cgcagaaaac
      121 tatccgcacg ttcgtttgtt gcatcaggcg aatgctggcg catcggtggc gcgtaatcat
      181 gggattgaag tggcaacggg caaatatgtc gcttttgtcg atgctgacga tgaagtctat
      241 cccaccatgt acgaaacgct gatgaccatg gcgttagagg acgacctcga cgtggcgcag
      301 tgcaacgctg actggtgttt tcgtgaaacg ggagaaacct ggcaatccat ccccagcgat
      361 cgccttcgct caaccggtgt attaaccggc ccggactggc tgcggatggg gctttcttcg
      421 cgccgttgga ctcacgttgt ctggatgggg gtttatcgcc gcgatgttat tgttaaaaat
      481 aacattaaat ttattgccgg attacatcat caggatattg tctggacaac agaattcatg
      541 tttaa
//
LOCUS       DAZKFU020000731          544 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.741, whole genome shotgun sequence.
ACCESSION   DAZKFU020000731 DAZKFU020000000
VERSION     DAZKFU020000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.741"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(320..>544)
                     /locus_tag="ACHOQQ_RS26750"
                     /old_locus_tag="pgaptmp_005350"
     CDS             complement(320..>544)
                     /locus_tag="ACHOQQ_RS26750"
                     /old_locus_tag="pgaptmp_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4473448.1"
                     /translation="NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQI
                     SLLAKEKPVEGSPLFLLQMCTEQLVVSGKI"
BASE COUNT          155 a          119 c          113 g          157 t
ORIGIN      
        1 acaaacagat attgataact tccctgacaa gttattaggt atcccacttg aagattgaga
       61 atggctccag agtaaactgt gtactgttcg ccggagtgtc attactctca acaatcggat
      121 ttgacagtga gtcgggcgct tcataccggt ttctacctgg agtcattgtc acttctccca
      181 actggataaa tatatcgcca attcacaaag tgggccaaaa tcaggtgaaa acgaattatt
      241 acgcctggta aaggctatag tacaactaag tgtggcgaat caggcagtct tgcatttgtc
      301 atcaatctca tgtcttacat caaatttttc cactcaccac tagttgttcc gtacacattt
      361 gtagtaaaaa cagtggactg ccttctaccg gcttttcttt agccagcaac gaaatctgtc
      421 gatagaaggt tggttcgaga ggaacggaac acaattcatc tgcaatggtt ttcagcgtta
      481 actctggtaa taaggcgatg cctaatccct gtcgaataaa actgatcgct gtatccgggt
      541 gatt
//
LOCUS       DAZKFU020000732          542 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.742, whole genome shotgun sequence.
ACCESSION   DAZKFU020000732 DAZKFU020000000
VERSION     DAZKFU020000732.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.742"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(371..>542)
                     /locus_tag="ACHOQQ_RS26755"
                     /old_locus_tag="pgaptmp_005351"
     CDS             complement(371..>542)
                     /locus_tag="ACHOQQ_RS26755"
                     /old_locus_tag="pgaptmp_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473449.1"
                     /translation="VAKTITAVGSTIDSTEKEISLQAEKQGKSYKILGAFFKNRVYMI
                     AKLTPVSKNDAS"
BASE COUNT          160 a          102 c          100 g          180 t
ORIGIN      
        1 taaaatatgc atccttttca agctgttcag cggcataaga aaacgtatta aatatgaatt
       61 cgacgattta accatttctg tctgattggt aatcatctgt gattaatgct ggtctattga
      121 ttacgccagc attaaccgct tagggatgta ttcttcctgt tgatatacag ccctcgaaaa
      181 agagagaaaa taaaactgtg tccaatatta ctctaccact ccagaccgaa gcatcggtag
      241 aattagcaga acgactggta caatagattg ggatagcgtc atttgtcagg tattccattg
      301 cctgacaaat gacaattttg agtcaataga cgtgcttctt gatcatgtct taaaaattat
      361 accatgagcc ttatgaagca tcatttttac tgactggtgt taattttgct atcatataaa
      421 ctctgttctt aaaaaacgcg cccagaattt tatatgattt cccttgtttt tctgcctgta
      481 aagaaatttc tttttcagtg ctgtcaatgg ttgaaccgac ggcagtaatc gttttggcga
      541 cg
//
LOCUS       DAZKFU020000733          541 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.743, whole genome shotgun sequence.
ACCESSION   DAZKFU020000733 DAZKFU020000000
VERSION     DAZKFU020000733.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.743"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(329..>541)
                     /locus_tag="ACHOQQ_RS26760"
                     /old_locus_tag="pgaptmp_005352"
     CDS             complement(329..>541)
                     /locus_tag="ACHOQQ_RS26760"
                     /old_locus_tag="pgaptmp_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGU4473450.1"
                     /translation="VNLSVESGPSCPLCNGPTVKRKGKSGFFWGCSRYPDCRGIAGEQ
                     GNSRGRKRKINFSHKQKSGDVGDKLN"
BASE COUNT          130 a          153 c          128 g          130 t
ORIGIN      
        1 gtagccaccc gaaggcgctc ctctcaggcc acttgtggag cattggtggt cgccggagcg
       61 acgttatgtg cccggacaga ctgaaatcac tgatggaaaa agtcaacagc taaccaaaaa
      121 caaaaaagca ccggcaagcc ggtgcttcag ggggatacca aatcccggtg ttgattctca
      181 tcttctccgg agagagcgac tcccggcacg gttttcacgg agtgcatgtg ccttgtaacc
      241 ccgccgtgtg cataacgggt taccggacct ctcagggctg tcagccccac tgtggtcaac
      301 agggtgatac cagaactcgc ctgcaatttt aattcagttt gtctccgaca tcaccagatt
      361 tttgtttatg agaaaaatta atttttcttt tcctgcccct gctgtttccc tgctcaccag
      421 caattcccct gcagtcggga taacgggagc atccccagaa aaaaccgctt tttcccttcc
      481 gtttcaccgt cggaccgtta cagagtggac atgaaggacc tgattccact gacagattaa
      541 c
//
LOCUS       DAZKFU020000734          540 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.744, whole genome shotgun sequence.
ACCESSION   DAZKFU020000734 DAZKFU020000000
VERSION     DAZKFU020000734.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.744"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..456
                     /locus_tag="ACHOQQ_RS26765"
                     /old_locus_tag="pgaptmp_005353"
                     /pseudo
     CDS             56..456
                     /locus_tag="ACHOQQ_RS26765"
                     /old_locus_tag="pgaptmp_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          143 a          127 c          130 g          140 t
ORIGIN      
        1 cggatcgtgg atggcagaaa acgttcagga tcaggtatcc attttaaacc agataatgag
       61 taagtttacc ccatccatgc cccacgcagt gagatcggat gtcattaaaa aagtcaggtc
      121 agcaggtcag tatgggaagg cgaacaactc gcaggaatgg gtgcgctgaa gttgctggat
      181 gataaacatg gcgaactgaa atcaatgcgt accgcgccaa attatttacg tcatggtgtc
      241 gccggtcgga ttttacgcca cattttgcag gtcgcccatg acagatgcct tcatcgcctg
      301 agtttagaaa cgggtacaca ggctggattt acggcctgcc atcaacttta tttgaagcat
      361 ggtttcgctg attgcgaacc gtttgctgat tatcgacttg atccacacag tcgattttta
      421 tcattgacgc tatgcgaaaa taatgaattg ccatgagcca gacgcagcac attcttgcat
      481 tcgacgtgct gcgcctttat ttatcaccag caggaaacgc cttgtccata gacgcccctt
//
LOCUS       DAZKFU020000735          539 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.745, whole genome shotgun sequence.
ACCESSION   DAZKFU020000735 DAZKFU020000000
VERSION     DAZKFU020000735.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.745"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..66
                     /locus_tag="ACHOQQ_RS26770"
                     /old_locus_tag="pgaptmp_005354"
     CDS             <1..66
                     /locus_tag="ACHOQQ_RS26770"
                     /old_locus_tag="pgaptmp_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473451.1"
                     /translation="SFFKNVDFIHESNSYTVGMGV"
BASE COUNT          162 a          106 c          131 g          140 t
ORIGIN      
        1 tcgtttttta aaaatgtcga tttcattcat gaatctaact cgtataccgt agggatggga
       61 gtctgaagag taaaactcat taggattatc agaattcggc caatgcattg caaaaatgaa
      121 atacaaagag agatgtgtgc aggcgcttag ccgcgagagg catggcgaag ccatacagaa
      181 agcaccagca ttcagaactc ctgtgaaagc ggcatagtgc cgctgctcag gagttttgtt
      241 atgacccgta tcgtgctgag cgttgccacc ctatgatgcg attaaaaaaa ctcaccgtcg
      301 agccgatgat tgacgtcaat gctatcggat gatgccgggg gtgttccgga aggaattcct
      361 tgtttcccga actgttacca acaaaattat aacgatgagc aagtaaagac cggttggtgg
      421 cagtactgac cggtctgtga tggaagatta atttcacctt aaatcctggt gctgttagga
      481 tgctgacatt tttcgggtac accattcaga ttatgcaaat aaagatccaa tgaaaaaat
//
LOCUS       DAZKFU020000736          539 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.746, whole genome shotgun sequence.
ACCESSION   DAZKFU020000736 DAZKFU020000000
VERSION     DAZKFU020000736.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.746"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>539)
                     /locus_tag="ACHOQQ_RS26775"
                     /old_locus_tag="pgaptmp_005355"
     CDS             complement(<1..>539)
                     /locus_tag="ACHOQQ_RS26775"
                     /old_locus_tag="pgaptmp_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473452.1"
                     /translation="LQKSYCPCLDYSGTESDEAAVKRYLREYLAELGFNREDFRENGK
                     MYELGKYQGTIRKGYGKRKSLMKQYIKACKNEYKKDYREKKLDKSELDRILNNIDKFY
                     YAFYSLSILLDMQRKVKILDSIAYYLRVLIREGLWVHGLYGYAARYLYDFNIFDTTPY
                     ARALLERFHEFESGPKGAL"
BASE COUNT          150 a          128 c           80 g          181 t
ORIGIN      
        1 ttaatgctcc tttggggcca ctctcaaact catgaaaccg ttcaagtaat gctcttgcat
       61 aaggcgttgt gtcaaaaata ttaaaatcat aaagatagcg tgcagcgtaa ccatacaacc
      121 catgtaccca aagcccttct cttatcagca ctctgagata atatgcaata ctgtcaagta
      181 tctttacctt ccgttgcata tcaagcaaaa tacttaatga ataaaatgca taatagaact
      241 tgtcaatgtt gttgagtatt ctgtccagct cactcttatc aagcttcttc tctctgtaat
      301 ccttcttgta ttcattctta caggccttta tatattgttt cataaggctc ttccttttac
      361 cgtacccttt tcgaatagtt ccctggtatt ttcccagctc atacattttt ccgttttccc
      421 gaaaatcttc tctgttaaat cccagttcag caagatactc ccggagataa cgtttaacgg
      481 cagcttcatc gctttctgta ccagagtaat ccaggcaggg acaatatgat ttttgcaaa
//
LOCUS       DAZKFU020000737          533 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.747, whole genome shotgun sequence.
ACCESSION   DAZKFU020000737 DAZKFU020000000
VERSION     DAZKFU020000737.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.747"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>533
                     /locus_tag="ACHOQQ_RS26780"
                     /old_locus_tag="pgaptmp_005356"
     CDS             <1..>533
                     /locus_tag="ACHOQQ_RS26780"
                     /old_locus_tag="pgaptmp_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4473453.1"
                     /translation="DALESGTIMTASCPRWLSLDDKRTAFIPDPDRVKTIELIFKLRM
                     ERRSLNAIAKYLNDHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISE
                     IAGYYPRVISDDLFYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAV
                     SGSLHGYYVCPMRRLHR"
BASE COUNT          163 a           86 c          129 g          155 t
ORIGIN      
        1 gatgcactgg agtcaggcac gattatgacg gcgtcttgtc cgagatggct ctcattggat
       61 gacaaaagaa cagcttttat tccagacccc gacagggtga aaacgattga gctaattttt
      121 aaactcagga tggaaaggcg atcattgaat gcaatagcca agtatttaaa tgatcatgct
      181 gtaaaaaatt tctcaggaaa agaaagtgca tgggggcctt ctgtaattga aaaattatta
      241 gcgaataaag ctctgatagg tatatgcgta ccttcatatc gtgcaagagg taaaggaata
      301 agtgaaatcg ctggctatta tcccagagtc atatcagatg atttgtttta cgctgtgcag
      361 gaaattcggt tggcaccttt tggtattagc aatagtagca aaaatcctat gttgataaat
      421 ctacttcgaa cagttatgaa gtgcgaggct tgtggtaata ccatgattgt tcatgcggta
      481 tctggaagtt tgcatggcta ttatgtttgt ccgatgagaa gactgcatcg atg
//
LOCUS       DAZKFU020000738          527 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.748, whole genome shotgun sequence.
ACCESSION   DAZKFU020000738 DAZKFU020000000
VERSION     DAZKFU020000738.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.748"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          157 a          110 c          106 g          154 t
ORIGIN      
        1 atcgacgaaa aaaccacttg atttgacagg aacacattca aaaaaatttc ttttttcatt
       61 tcaggagcga ttttcatctg ttacttgaag tatctgtgag attaagtgac tccccctaac
      121 gtcgtactaa atagtaatta ctggccatac cgggagcagt attcggcgct ccccacatgc
      181 agcagaataa ccgcatcagc gaagatgtat tgtattttta ctatcacaat gtgtacggac
      241 ggtttatgga aaagaatgaa cattagatac gcgatgcggc ggcaggttac acggttatca
      301 ctacgatagc caatactggt tggtgcatta ccgccgtgaa caataggacg ccgtactgct
      361 ggaaatttct tacctttatg actccccgcg ccgccgtatc gggaaacggg aagcaatcac
      421 agggaaagat catcagaagt tcactttttg tactaaataa ttcgcatttt atgtttaaaa
      481 attgagatat tcctcattac ctgaagctgt tttttattgc ttataca
//
LOCUS       DAZKFU020000739          524 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.749, whole genome shotgun sequence.
ACCESSION   DAZKFU020000739 DAZKFU020000000
VERSION     DAZKFU020000739.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.749"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..429)
                     /locus_tag="ACHOQQ_RS26785"
                     /old_locus_tag="pgaptmp_005357"
     CDS             complement(34..429)
                     /locus_tag="ACHOQQ_RS26785"
                     /old_locus_tag="pgaptmp_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4473454.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAAIPALAGMITTM
                     VTQGYEYHRDDDMALWSSADLTYSITYEM"
     gene            complement(426..>524)
                     /locus_tag="ACHOQQ_RS26790"
                     /old_locus_tag="pgaptmp_005358"
     CDS             complement(426..>524)
                     /locus_tag="ACHOQQ_RS26790"
                     /old_locus_tag="pgaptmp_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473455.1"
                     /translation="AFKQNIERIRRERLPEELAYALKQQLRIAIKR"
BASE COUNT          120 a          154 c          119 g          131 t
ORIGIN      
        1 ggcgccaggg gatttggtgt tgccatatca tcctcacatc tcgtatgtaa tggaataagt
       61 cagatctgca gagctccaca atgccatatc gtcatcacga tgatactcat agccctgcgt
      121 aaccatcgtg gtaatcatgc ctgccagtgc agggatcgcc gccatcgccg ggtaaatccg
      181 gctttccatc cactgatcaa gctctgaatc cggtacctgt gccggtaaaa acacctcaat
      241 atgcagtgtg gcccgccagg tatcagcatc cagctcttca ccggtatact ctgcatccgt
      301 cagataaacc gcgaccgccg gaaaatccgc ctcctcaaaa acagcggggc gaccatcaaa
      361 cagcgtcgcc ccggtgtcat gcttctccag tgcatccagt acggctgcac ggatatcagt
      421 atgtttcatc gttttatcgc aatcctcagt tgttgtttca gcgcgtatgc cagttcttct
      481 ggcaggcgtt cacgccggat acgttcaata ttctgtttaa atgc
//
LOCUS       DAZKFU020000740          524 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.750, whole genome shotgun sequence.
ACCESSION   DAZKFU020000740 DAZKFU020000000
VERSION     DAZKFU020000740.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.750"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            242..>524
                     /locus_tag="ACHOQQ_RS26795"
                     /old_locus_tag="pgaptmp_005359"
     CDS             242..>524
                     /locus_tag="ACHOQQ_RS26795"
                     /old_locus_tag="pgaptmp_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGU4473456.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEE"
BASE COUNT          162 a          102 c          115 g          145 t
ORIGIN      
        1 ttcgcccttt gattagcaac tctggttttc tttcttagct gaacaatctg gttatagcgt
       61 gcggactgac cgttaatttt ctccatctta ctggataaca caccatgagc tgctaccttt
      121 aaaacacctg aatgtcaaca ggttaactcg tgccatatcg ttataatccc tttgatatta
      181 gatgcaaatt aaggtcatat atagccttat cgataacatg gttaatttta aggataagaa
      241 tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg tatcagcgcc
      301 aacaccaagt tcgatgaatg aaagcacggc gaagggaatc tttaaatatt taaaagaact
      361 gggagtaccc gcgagtgccg ctgatattac ggcgcgagct gaccaggaag gctggaaccc
      421 tgggttcaca gaaaaaatgg ttggatgggc aaaaaaaatg gagacaggtg aacgctctgt
      481 gattaaaaat cctgagtact tttcaacata tatgcaggaa gaac
//
LOCUS       DAZKFU020000741          517 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.751, whole genome shotgun sequence.
ACCESSION   DAZKFU020000741 DAZKFU020000000
VERSION     DAZKFU020000741.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.751"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..503
                     /locus_tag="ACHOQQ_RS26800"
                     /old_locus_tag="pgaptmp_005360"
     CDS             <1..503
                     /locus_tag="ACHOQQ_RS26800"
                     /old_locus_tag="pgaptmp_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4473457.1"
                     /translation="CISQPKKTTLRDKAMVNYAFDYLSSPGSLPFTTAATELSAIHGH
                     STSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDT
                     RKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAERLL
                     AGLPAH"
BASE COUNT          157 a          122 c          115 g          123 t
ORIGIN      
        1 cctgtattag ccaacccaag aaaacgacgt tgcgtgataa agccatggtg aattatgcct
       61 ttgattattt aagctcaccg ggcagtcttc cattcaccac ggcagccacg gagctttccg
      121 cgattcatgg tcactcaacg tcgcaatatc gccttggaga attttatctt catggtagcg
      181 acggtaaacc actggattat acacaggcga gatactggta tgagcaatca gcggaacagg
      241 aaaatccacg cgcgcaaagt aaactggggt ggatctacct caaaggtctg ggggtcaaac
      301 ccgacacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg
      361 ctcaatatac tttaggtttg atctacagaa atggctcagg tattaatgtt aaccattatg
      421 aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac
      481 ttgccgggct tcccgcacat taacgtcgac tgtcacc
//
LOCUS       DAZKFU020000742          516 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.752, whole genome shotgun sequence.
ACCESSION   DAZKFU020000742 DAZKFU020000000
VERSION     DAZKFU020000742.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.752"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>516)
                     /locus_tag="ACHOQQ_RS26805"
                     /old_locus_tag="pgaptmp_005361"
     CDS             complement(<1..>516)
                     /locus_tag="ACHOQQ_RS26805"
                     /old_locus_tag="pgaptmp_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HGU4473458.1"
                     /translation="LPTYLIDFLIGLNLTLAILVFLGSFYVDRILSFSSFPSILLITT
                     LFRLALAISTSRLILLEADAGEIITSFGEFVIGDSLVVGFVIFSIVTIVQFIVITKGS
                     ERVAEVAARFSLDGMPGKQMSIDADLRAGIIDADLAKERRSVLERESQLYGSFDGAMK
                     FIKGDAIANII"
BASE COUNT          184 a          117 c           90 g          125 t
ORIGIN      
        1 taataatgtt tgcaatagca tcacctttga taaatttcat cgcaccatca aaggaaccat
       61 acagctgact ctctcgttct aatacgctac gacgttcttt tgctaaatct gcatcaatga
      121 tcccagcgcg tagatcggca tcaatgctca tttgtttacc tggcataccg tcaagcgaaa
      181 agcgggcagc aacttcagcc acacgttccg aacctttagt aatcacgata aactgaacaa
      241 tagtaacaat ggagaaaata acaaaaccaa ctaccaggct atctccaata acgaactcac
      301 caaaactcgt tataatttct ccagcatccg cctcaagaag tataagtcga ctggtactga
      361 tcgccagcgc caaacgaaat aacgttgtga taaggaggat tgatggaaat gaagaaaagc
      421 ttaagatcct atcaacatag aatgatccta aaaatacaag tattgccaat gttaagttaa
      481 gcccaatcaa aaagtcgatt aaataggtgg gcaatg
//
LOCUS       DAZKFU020000743          513 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.753, whole genome shotgun sequence.
ACCESSION   DAZKFU020000743 DAZKFU020000000
VERSION     DAZKFU020000743.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.753"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>513)
                     /locus_tag="ACHOQQ_RS26810"
                     /old_locus_tag="pgaptmp_005362"
     CDS             complement(<1..>513)
                     /locus_tag="ACHOQQ_RS26810"
                     /old_locus_tag="pgaptmp_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU4473459.1"
                     /translation="CILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRTEI
                     STPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAGFV
                     NTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSSYL
                     ALMEFSGNAMT"
BASE COUNT          163 a          110 c          111 g          129 t
ORIGIN      
        1 ggtcatggca tttccactaa actccattaa cgccagatat gatgaaacca gtgagtgacg
       61 actgatttgc attccggaac gttccagcgc tgcgacacgt tgcagagtgg tataactgct
      121 gtccgttgtc atggaaacag ttgtcacacc gggcactgat atatgtgcaa aatcagaaaa
      181 tctgtagaaa gtatttgttg ccgtattaac gaatccggct acatataaat tattttgctc
      241 aacaatcaga cgaagatggt caaaacgcgc ctgatagaca tcaagccctc gtatatccac
      301 agcaaaataa ctgcccggtg gggtgtggtt aataacagac accgatgtgg tcccctgaga
      361 tatatgctca agaggagtcg atatttctgt ccgtatacta tttaacgaag agacataact
      421 ttgttgagtc gaaaagtcta tcgtaaattc ccgggaatag gataccgaag aaaagcccag
      481 taacaggcac agtacccatt ttaacaatat aca
//
LOCUS       DAZKFU020000744          509 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.754, whole genome shotgun sequence.
ACCESSION   DAZKFU020000744 DAZKFU020000000
VERSION     DAZKFU020000744.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.754"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>509
                     /locus_tag="ACHOQQ_RS26815"
                     /old_locus_tag="pgaptmp_005363"
     CDS             <1..>509
                     /locus_tag="ACHOQQ_RS26815"
                     /old_locus_tag="pgaptmp_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HGU4473460.1"
                     /translation="MMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEKLRTP
                     PEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAVGENS
                     ESLRAREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAGLQPV
                     RPEFDVGPL"
BASE COUNT          115 a          126 c          176 g           92 t
ORIGIN      
        1 atgatgccgg tgatgaccga ttttgcagaa agcctctggt tcaccccacc ggtatctgat
       61 ccggaaaacg gcgtgtggtg gctggacggg cttccgcatg ccgctgttgt ggtggagaaa
      121 ctgcgcacac caccggagcc gggaaccatc acaggggaac aggcccgggg cgaaaagaca
      181 gtgaatgccc tgatggacac ctttccggag ggaacggtgc tgtgtatgac aattgtggtc
      241 cagccacagg acacactgga agagcgcttc acgaggttgt cgaaaaatgc ggtcggggag
      301 aacagtgaat ccctgcgtgc ccgggaggat gtggcgaccg tgaagaaata tctgggaaac
      361 cggcacaagc tctaccgtgc cgggatcacg tttctgctgc gggcagagga tatggagagt
      421 ctgaagcgca gacgggttga actgacgacg gtgctgctgg gcgcgggctt acagccagtg
      481 cgcccggagt ttgatgtcgg accgctgaa
//
LOCUS       DAZKFU020000745          509 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.755, whole genome shotgun sequence.
ACCESSION   DAZKFU020000745 DAZKFU020000000
VERSION     DAZKFU020000745.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.755"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          151 a           78 c          103 g          177 t
ORIGIN      
        1 tggttgatta tgtaatatca ccggctgatg atattaaaaa cgtcatgatg ttgtatggaa
       61 ataatgctga aaacaagaac agacgcagac tgatctacaa cgcattaacc atcatttgtg
      121 gtgatgatac taaaaacaac cagtcagacc ctagatgata ttatcggaac gtgtttttgg
      181 catccgatgt ttgtatatcc ctgctattgt tgctagtcgg ctagcaacaa tagcagaggg
      241 ggcatgcatg tctgaaaaaa taaaaaatcg caacacattg atttttatct tgtttgcatt
      301 ttgcaaatac tatctctcgt ttttgtgatt agtgtcatgt tttatcgtct cgcggataac
      361 cgtgaggagt gaacagtaac cctgattata tgatgaattg ttcctgtatt gtatgtgtga
      421 aatatgcttc tggtgatatg aaaacatatc cataacgtgg tgtttcacgc tgtattttaa
      481 aattttttat tgggattgat attttattt
//
LOCUS       DAZKFU020000746          505 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.756, whole genome shotgun sequence.
ACCESSION   DAZKFU020000746 DAZKFU020000000
VERSION     DAZKFU020000746.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.756"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            219..>505
                     /locus_tag="ACHOQQ_RS26820"
                     /old_locus_tag="pgaptmp_005364"
     CDS             219..>505
                     /locus_tag="ACHOQQ_RS26820"
                     /old_locus_tag="pgaptmp_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU4473461.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRD"
BASE COUNT          161 a           85 c          102 g          157 t
ORIGIN      
        1 atgaagtaat ttaaattaac tatcaaaaat cgctcatttc tgcttattga tagaaagatt
       61 gatcgccagt ctgtttagag cggtgcagca gcaaactttc ataaccaggt taatcctgag
      121 tttgcagtac gaaaagttaa aaatgagcga tttctgtaag attccccatt ttgccaggct
      181 tatgattcgc agatcattca agtgcaaagg agcgatgtat ggattgttca aaatgcaatg
      241 gttatgcgac gatgttatta aatatggtgc agggttctga cccggttaat ttactggaat
      301 tacatggttt ccttgaacat ttcgcatatt atgtttcatt tggcaaattt aatgctggcc
      361 accagcgcta taatgcattt aaaaaattcg tcagtgagat ttcggagatc agcgcaaacg
      421 atatcaatat gacgattaaa accgggcaat ccagacatga aaatgttatc tctataaata
      481 tgaatgatgc aattcctcgt gatga
//
LOCUS       DAZKFU020000747          505 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.757, whole genome shotgun sequence.
ACCESSION   DAZKFU020000747 DAZKFU020000000
VERSION     DAZKFU020000747.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.757"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..>505
                     /locus_tag="ACHOQQ_RS26825"
                     /old_locus_tag="pgaptmp_005365"
     CDS             87..>505
                     /locus_tag="ACHOQQ_RS26825"
                     /old_locus_tag="pgaptmp_005365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473462.1"
                     /translation="MGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIVLDGYSVSL
                     GNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRRITNLAQGK
                     DDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSG"
BASE COUNT          158 a          111 c          123 g          113 t
ORIGIN      
        1 gagaaaaaaa caatacaaca acacgattct ccggaaatat ttacggcagt gaaaatactg
       61 tcacatcaga gtcaaacagt ctggttgtgg gtaacagtaa ttccgtggag ggagggtata
      121 acaacattgt tattggtaat tcctccacgg tcaaagcttc cggtttgatc agaaaagtgg
      181 gcgaagatat tgtgctggat ggttacagcg tcagcctcgg taatgccacc agaattaacg
      241 gtgattcatc tgttgccata ggtctgacat cgtccgtaac cgggaacgac tccgtggcac
      301 tgggcagtca ttccagtgct aacggaaaca atatcgtatc cgtcggctct gacacactga
      361 aacgccgcat cacaaatctg gcacagggga aagatgacca tgatgcggta aatctgatgc
      421 agatgaccag tgctgttaac cgggaagctg gagaaagaat aaaatcaaca aacaacctgc
      481 agaatcagct taataccctc tctgg
//
LOCUS       DAZKFU020000748          504 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.758, whole genome shotgun sequence.
ACCESSION   DAZKFU020000748 DAZKFU020000000
VERSION     DAZKFU020000748.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.758"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..473)
                     /locus_tag="ACHOQQ_RS26830"
                     /old_locus_tag="pgaptmp_005366"
     CDS             complement(<1..473)
                     /locus_tag="ACHOQQ_RS26830"
                     /old_locus_tag="pgaptmp_005366"
                     /inference="COORDINATES: protein motif:HMM:NF025079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HGU4473463.1"
                     /translation="MEPKYIFDELLAFLGQRRIALPGYSTVQNLVSDALNFERKRTIN
                     ILSKRIYPATAQKITSIIHDDGILNSIRGYNYSTRDFSASEIENELRTHNVISSIYYE
                     IKDIINSLGISQGNMEYYATIVKHSTTFRLKRHPKWQGILYLCCYLYFRYREIND"
BASE COUNT          149 a           88 c           82 g          185 t
ORIGIN      
        1 ttatcattta tctctctgta tctgaagtac aagtagcagc acaaataaag tattccttgc
       61 catttagggt gtcgcttaag tctgaatgtt gtactgtgtt ttacgatagt tgcatagtac
      121 tccatgtttc cctgggatat acctaatgaa ttaattatat cttttatttc atagtaaata
      181 ctacttatca cattgtgagt tcttaattca ttttctattt ctgatgctga gaagtctctc
      241 gtgctataat tatatcccct gatggaattt aatattccat catcatggat gatggatgta
      301 atcttttgtg cagtggcagg ataaattctc tttgacaaaa tattaattgt ccttttcctt
      361 tcaaagttta aagcatctga aacgaggttc tggacagtac tgtatccagg aagagctata
      421 cgccgctgtc ctaaaaaagc aagtaattca tcaaaaatat attttggttc catacatata
      481 gtagcaacat cattcagacg ttca
//
LOCUS       DAZKFU020000749          499 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.759, whole genome shotgun sequence.
ACCESSION   DAZKFU020000749 DAZKFU020000000
VERSION     DAZKFU020000749.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.759"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>499
                     /locus_tag="ACHOQQ_RS26835"
                     /old_locus_tag="pgaptmp_005367"
     CDS             <1..>499
                     /locus_tag="ACHOQQ_RS26835"
                     /old_locus_tag="pgaptmp_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473464.1"
                     /translation="SCVALSACSAQEIAELNRQISDGAFAVTKVLNVGVNQADDDLKY
                     LSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNNGHANGKWAAAA
                     ISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYITYASPAPGHLKD
                     KFIEP"
BASE COUNT          152 a           88 c          141 g          118 t
ORIGIN      
        1 cttcgtgtgt tgcgctgagt gcctgttctg cacaggaaat tgcagagctg aacaggcaga
       61 taagtgacgg tgcatttgct gtgacaaaag tactgaacgt aggtgtaaac caggcagatg
      121 atgatctcaa atatctgtct caggcacccc gggcaaaaag agagaaacgt gatcatgtca
      181 tagaagtccc tgttgatgtg gatacagctg cgggaagact gaaacggtac tataagtttt
      241 attcaaatga agaagtcgat gcgattcgta ataacggaca tgcgaacggg aagtgggccg
      301 cagcagcaat aagtcagaac gggcatatgt gggatgccat gcccggtagt tattataaaa
      361 tgggcagtga ctgggatggt ggcgagtttg atgatcatct gacgcttgaa cttgaaaaaa
      421 atggcaaagg aagtcggata tatataacgt atgcatctcc ggcaccgggg catctgaaag
      481 ataaatttat tgagccatt
//
LOCUS       DAZKFU020000750          494 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.760, whole genome shotgun sequence.
ACCESSION   DAZKFU020000750 DAZKFU020000000
VERSION     DAZKFU020000750.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.760"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>494
                     /locus_tag="ACHOQQ_RS26840"
                     /old_locus_tag="pgaptmp_005368"
     CDS             <1..>494
                     /locus_tag="ACHOQQ_RS26840"
                     /old_locus_tag="pgaptmp_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4473465.1"
                     /translation="LLPFIPANAGILLVPCCRGASAFTTGADGTYSESAGASENSLRW
                     GVGKPLYQDLVSRTKAALAKNPKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQFR
                     TELAGQASQSTGGSASAVPWICGDTTYFWKQRHAEGYASVYGGYKGKESQNIFFVPLM
                     TDEN"
BASE COUNT          114 a          107 c          170 g          103 t
ORIGIN      
        1 aactgctgcc gtttataccg gcgaatgcgg gcattctgct ggttccgtgc tgccgtggtg
       61 cttcggcatt cacaacgggg gcagacggca catacagcga atcagccggt gcatcggaaa
      121 attcactgcg ctggggtgtg ggtaagccgc tgtatcagga tttggtgagc cggactaaag
      181 ccgcactggc gaagaacccg aaaaaccgtc tgcttgcggt ggtgtggatg cagggagaag
      241 gtgatgcggc agtggggacg catgcgcagc atccggggct gtttagtgcg atggtgaatc
      301 agttcagaac ggagcttgcc ggtcaggcat cacagagcac aggaggcagc gcatcagcgg
      361 taccgtggat ttgcggggac acaacgtatt tctggaagca gcgtcatgcg gaagggtatg
      421 catcagtata cggagggtat aagggtaagg agtcgcagaa cattttcttt gtaccgttaa
      481 tgacggatga gaat
//
LOCUS       DAZKFU020000751          492 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.761, whole genome shotgun sequence.
ACCESSION   DAZKFU020000751 DAZKFU020000000
VERSION     DAZKFU020000751.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.761"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>492
                     /locus_tag="ACHOQQ_RS26845"
                     /old_locus_tag="pgaptmp_005369"
     CDS             <1..>492
                     /locus_tag="ACHOQQ_RS26845"
                     /old_locus_tag="pgaptmp_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4473466.1"
                     /translation="RMQPGSETEPGVETQSSVLKMLSSDSELSEKQGQKILSSEIEPS
                     EKSEGYHRVRKPNHYVRNITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELA
                     QGVLESLSGMLGSHSLQNPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRA
                     TAQS"
BASE COUNT          143 a          106 c          150 g           93 t
ORIGIN      
        1 cagaatgcag ccgggttcgg aaaccgaacc gggggtagaa acacagagtt cggtattgaa
       61 aatgctgagt tcggattccg aactcagtga aaaacagggg caaaaaatac tgagttcgga
      121 aatcgaacca tcagaaaaat cagaaggtta tcacagagtt cggaaaccga accattacgt
      181 acgtaatatc acacacagtg tgattaaaaa cacgtacgta ttgcctgaag cgctgacagg
      241 gcaaatcagg gaggaggagg ccattatgct gaccagtcag ttgcaggcgc tgccggaaga
      301 actggcgcag ggggttctgg aaagtctgtc agggatgctg ggcagccact cgctgcagaa
      361 tcctgtgggc tggttgcttg cagtgctgaa gcgagcccgg gagggaaatt tttatccgcc
      421 gaagcagaaa caacgtgttg ttgtgccagc ctccgtactg gcaggagaaa gggggcgagc
      481 tactgcgcaa tc
//
LOCUS       DAZKFU020000752          490 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.762, whole genome shotgun sequence.
ACCESSION   DAZKFU020000752 DAZKFU020000000
VERSION     DAZKFU020000752.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.762"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..424
                     /gene="ydeI"
                     /locus_tag="ACHOQQ_RS26850"
                     /old_locus_tag="pgaptmp_005370"
     CDS             32..424
                     /gene="ydeI"
                     /locus_tag="ACHOQQ_RS26850"
                     /old_locus_tag="pgaptmp_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGU4473467.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          136 a          102 c          124 g          128 t
ORIGIN      
        1 aagtcaaacc aaacgcaatt aaggagttag tatgaaattt caggccattg tattagcaag
       61 ttttcttgtc atgccttatg cgttggcaga tgaccagggc ggtttaaaac aggatgcagc
      121 gccaccgccg cctcatgcaa tagaagatgg ctatcgcgga accgatgatg caaaaaaaat
      181 gaccgttgat ttcgcaaaaa ccatgcacga tggcgcttcg gtttcactac gcggtaattt
      241 gatttctcac aaaggagagg accgttacgt ttttcgcgat aagagcggtg aaattaatgt
      301 tgttattcct gcggccgttt ttgatggacg agaagttcag ccggaccaga tgatcaacat
      361 tagcggcagt ctggataaga aaagcgcgcc ggccgtcgtt cgggtcactc atttgcagaa
      421 ataaaaataa attagcccga tggcctgaac tactgggcca ttagggtttg ggttatgctt
      481 tttgtcatcc
//
LOCUS       DAZKFU020000753          490 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.763, whole genome shotgun sequence.
ACCESSION   DAZKFU020000753 DAZKFU020000000
VERSION     DAZKFU020000753.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.763"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>490
                     /locus_tag="ACHOQQ_RS26855"
                     /old_locus_tag="pgaptmp_005371"
     CDS             <1..>490
                     /locus_tag="ACHOQQ_RS26855"
                     /old_locus_tag="pgaptmp_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473468.1"
                     /translation="SKFNILDMLDNLTMRQQVVMEEFLRVFTSMYKASAMSEAIMSNV
                     FEQLVQYQRKETERATAASSVGVIIGSGVGAAGAAASVVANYRSHPEKMVTLTPDQEH
                     TINRTDDDVQRASKREVREQQASLKQIDEKIKNTTKDTSLNEEQKEEALKSYDKEKET
                     TLE"
BASE COUNT          182 a           88 c          107 g          113 t
ORIGIN      
        1 tctaaattta atatccttga tatgctagat aatttgacca tgcgacaaca ggttgtaatg
       61 gaggaatttt taagggtatt cacatcaatg tataaagcct ctgctatgtc tgaggctatt
      121 atgagtaatg tttttgaaca acttgtccag tatcagagaa aagaaaccga acgcgccaca
      181 gcagcaagtt cggtaggtgt tattatcggg tcaggagttg gcgcagccgg ggccgcggca
      241 tctgtcgtag caaattacag aagccatccc gaaaagatgg taacccttac acctgaccaa
      301 gagcatacta tcaatagaac tgatgatgat gtacagcgcg cgtcaaaaag ggaagttcga
      361 gagcaacaag cttctctcaa acaaatagat gaaaaaataa aaaatacaac gaaagatacg
      421 agtcttaatg aagaacaaaa agaagaggcg ctgaaatctt atgataaaga aaaagaaact
      481 actctagaaa
//
LOCUS       DAZKFU020000754          482 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.764, whole genome shotgun sequence.
ACCESSION   DAZKFU020000754 DAZKFU020000000
VERSION     DAZKFU020000754.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.764"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..>482
                     /locus_tag="ACHOQQ_RS26860"
                     /old_locus_tag="pgaptmp_005372"
     CDS             34..>482
                     /locus_tag="ACHOQQ_RS26860"
                     /old_locus_tag="pgaptmp_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473469.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQVRIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVV"
BASE COUNT          126 a          108 c          151 g           97 t
ORIGIN      
        1 gttaaccggc gacgataaac gcagggtgaa attatggcga taaaagggct tgatcaggcg
       61 attgataatc tgagccgggt tcgtaaaaac gccattcctg ctgcttcagc aatggccatt
      121 aaccgcgtgg ccacaacggc aataaatcag tctgcgtcac aggttgcccg tgagacaaag
      181 gtacgccgga aactggtaaa ggaaagggcc aggctgaaaa gggccacggt aaaaaatccg
      241 caggtcagaa tcaaagttaa ccggggggat ttgcccgtaa tcaggctggg taatgcgcgg
      301 gttgttctgt cccgacgcag gcgacgtaaa aaggggcagc gttcatccct gaaaggtggc
      361 ggcagtgtgc ttgtggtggg taaccgtcgc attcccggcg cgtttattca gcaactgaaa
      421 aatgggcggt ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg
      481 gt
//
LOCUS       DAZKFU020000755          482 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.765, whole genome shotgun sequence.
ACCESSION   DAZKFU020000755 DAZKFU020000000
VERSION     DAZKFU020000755.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.765"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..>482
                     /locus_tag="ACHOQQ_RS26865"
                     /old_locus_tag="pgaptmp_005373"
     CDS             34..>482
                     /locus_tag="ACHOQQ_RS26865"
                     /old_locus_tag="pgaptmp_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473470.1"
                     /translation="MVIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQVRIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVV"
BASE COUNT          128 a          106 c          150 g           98 t
ORIGIN      
        1 gttaaccggc gacgataaac gcagggtgaa attatggtga taaaaggact tgatcaggcg
       61 attgacaatc tgagccgggt tcgtaaaaac gccattcctg ctgcttcagc aatggccatt
      121 aaccgtgtgg ccacaacggc aataaatcag tcggcgtcac aggttgcccg tgagacaaag
      181 gtacgccgga aactggtaaa ggaaagagcc aggctgaaaa gggccacggt taaaaatccg
      241 caggtcagaa tcaaagttaa ccggggggat ttgcccgtaa tcaggctggg taatgcgcgg
      301 gttgttctgt cccgacgaag gcgacgtaaa aaggggcagc gttcatccct gaaaggtggc
      361 ggcagtgtgc ttgtggtggg taaccgtcgc attcccggcg cgtttattca gcaactgaaa
      421 aatgggcggt ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg
      481 gt
//
LOCUS       DAZKFU020000756          480 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.766, whole genome shotgun sequence.
ACCESSION   DAZKFU020000756 DAZKFU020000000
VERSION     DAZKFU020000756.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.766"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>480
                     /locus_tag="ACHOQQ_RS26870"
                     /old_locus_tag="pgaptmp_005374"
     CDS             <1..>480
                     /locus_tag="ACHOQQ_RS26870"
                     /old_locus_tag="pgaptmp_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HGU4473471.1"
                     /translation="FTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLTSVK
                     DAQGRETRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRV
                     ISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYD"
BASE COUNT          114 a          143 c          150 g           73 t
ORIGIN      
        1 gttcaccgac tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac
       61 ggcggtccac cgcgaggaag gcatcagcct ttaccgccgc tatgacaacc gtggccggtt
      121 aacctcggtg aaagacgcac agggccgtga aacgcggtat gaatacaacg ccgcaggcga
      181 cctgactgcc gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg
      241 aaaggcggtc agcaccacgc agggcgggct gacgcgcagt atggagtacg atgctgccgg
      301 acgtgtcatc agcctgacca acgagaacgg cagtcacagc gacttcagct acgatgcgct
      361 ggaccgcctg gtacagcagg gcggctttga cgggcggacg cagcgttatc attatgacct
      421 gaccggaaaa ctcacacaga gtgaggatga gggacttgtc accctctggc actacgacga
//
LOCUS       DAZKFU020000757          477 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.767, whole genome shotgun sequence.
ACCESSION   DAZKFU020000757 DAZKFU020000000
VERSION     DAZKFU020000757.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.767"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>477
                     /locus_tag="ACHOQQ_RS26875"
                     /old_locus_tag="pgaptmp_005375"
     CDS             <1..>477
                     /locus_tag="ACHOQQ_RS26875"
                     /old_locus_tag="pgaptmp_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HGU4473472.1"
                     /translation="KSGSGTLTVSNTTLTQKAVNLNEGTLTLNDSTVTTDVIAQRGTA
                     LKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAGQIHFTSTRTGKFV
                     PATLKVKNLNGQNGTISLRVRPDMAQSNADRLVIDGGRATGKTILNLVNAGNSASG"
BASE COUNT          126 a          148 c          127 g           76 t
ORIGIN      
        1 aaaaatcagg aagtggcaca ctcactgtca gcaacaccac actcacccag aaagccgtca
       61 acctgaatga aggcacgctg acgctgaacg acagtaccgt caccacggat gtcattgctc
      121 agcgcggtac agccctgaag ctgaccggca gcactgtgct gaacggtgcc attgacccca
      181 cgaatgtcac tctcgcctcc ggtgccacct ggaatatccc tgataacgcc acggtgcagt
      241 cggtggtgga tgacctcagc catgccggac agattcattt cacctccacc cgcacaggga
      301 agttcgtacc ggcaaccctg aaagtgaaaa acctgaacgg gcagaatggc accatcagcc
      361 tgcgtgtacg cccggatatg gcacagagca atgctgacag actggtcatt gacggcggca
      421 gggcaaccgg aaaaaccatc ctgaacctgg tgaacgccgg caacagtgcg tcggggc
//
LOCUS       DAZKFU020000758          477 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.768, whole genome shotgun sequence.
ACCESSION   DAZKFU020000758 DAZKFU020000000
VERSION     DAZKFU020000758.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.768"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..477
                     /locus_tag="ACHOQQ_RS26880"
                     /old_locus_tag="pgaptmp_005376"
                     /pseudo
     CDS             14..477
                     /locus_tag="ACHOQQ_RS26880"
                     /old_locus_tag="pgaptmp_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150682.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          175 a           79 c           91 g          132 t
ORIGIN      
        1 caaaggagat tgtgtgacaa tagtaagaga ttctgttagt ggtgaaattg ttcatagtca
       61 tgatcttcgg gaaatggatg ttctttatat acaaaaaacc acttttgaat gtccgtatga
      121 caaatgtagg atacctgcta ctccatgtag cttcaaagaa ataaatttaa atcaatcata
      181 ctttcgatat aaagagcctc gtaggccggg atgtaagatt catgatccta agagtaaaat
      241 aagtataact caaagaataa taaaaagcat aattcaccac cggcactggc tatatatctt
      301 ctgaaacttg atgtacctga aggaaattac gactcttctg gcagaagaat taataaacag
      361 gcgcaagatg atgaaaaaga attggggggc aaagtacacc ctgtgtcatc ttcaagcatc
      421 aaaccggtag tagactacta cattaatgga gataatctct atgaaccatt atcaatt
//
LOCUS       DAZKFU020000759          472 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.769, whole genome shotgun sequence.
ACCESSION   DAZKFU020000759 DAZKFU020000000
VERSION     DAZKFU020000759.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.769"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            28..>472
                     /locus_tag="ACHOQQ_RS26885"
                     /old_locus_tag="pgaptmp_005377"
     CDS             28..>472
                     /locus_tag="ACHOQQ_RS26885"
                     /old_locus_tag="pgaptmp_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HGU4473473.1"
                     /translation="MRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAAK
                     ANFAVAVGRYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADRG
                     AGIEAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKIISAYNSQGT"
BASE COUNT          149 a           87 c          106 g          130 t
ORIGIN      
        1 actatataaa agagatggtg aattcaaatg cgtaaaatca gtgtcagtta tatatgcaaa
       61 tcattatttc tctactgctc agcttcttta cctgtcttat ctgcaaatgg tattcctgat
      121 aaaatgatcg tggaacttgg tgataacagt gcggcaaaag ctaatttcgc tgttgctgtt
      181 ggtcgatatt cacaggcaaa tgcagaaaaa agtatttctt taggtgcatc agcaagagct
      241 attgaaaata actctaccgc gttaggagca aacgcaacaa cattgaaacc attctctgtc
      301 gctctgggcg caggaagtgt tgctgatcgt ggcgctggta ttgaagcata tatgccagag
      361 acaaagcgct ggaatccagg ggatgtagta aaatccagga tgtctgacaa gcagcttaac
      421 aggcttatgg aaatcgataa gataatatcc gcttataatt cacaagggac gc
//
LOCUS       DAZKFU020000760          466 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.770, whole genome shotgun sequence.
ACCESSION   DAZKFU020000760 DAZKFU020000000
VERSION     DAZKFU020000760.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.770"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>466)
                     /locus_tag="ACHOQQ_RS26890"
                     /old_locus_tag="pgaptmp_005378"
     CDS             complement(<1..>466)
                     /locus_tag="ACHOQQ_RS26890"
                     /old_locus_tag="pgaptmp_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU4473474.1"
                     /translation="DYSRRYAINENIAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTE
                     EESFETALDVEHAIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYND
                     ICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDATNLINLKTLLNDG"
BASE COUNT          149 a           96 c           70 g          151 t
ORIGIN      
        1 accatcattg agtagtgttt taagattgat taaattagtt gcatcttcac gtaccatcgt
       61 ggcaacagga acaggcaatg acatgaaaac ttcttcattc accatcaagt gaatcatgca
      121 ttgcgttccc tttgtcatat ccacacttac gctacaaata tcgttgtaat gcgaagagtg
      181 catttcagga aacagattaa acacggtatc ttgaaaggca ctaaatcgtg ttaataaatc
      241 ctgataatga acaggttttg tttcaaaatt atcatcagca aatccaagca ctattgcatg
      301 ctctacatct aacgcagttt caaatgactc ttcttctgta aaatcattat ttaaaacctc
      361 acctgacgta ttatcactgt cataaaaagt gctcatcaat gcgatattgt ttgttacagc
      421 gtcttttgca atattctcat taattgcata tcgcctgcta taatca
//
LOCUS       DAZKFU020000761          466 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.771, whole genome shotgun sequence.
ACCESSION   DAZKFU020000761 DAZKFU020000000
VERSION     DAZKFU020000761.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.771"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            64..464
                     /locus_tag="ACHOQQ_RS26895"
                     /old_locus_tag="pgaptmp_005379"
                     /pseudo
     CDS             64..464
                     /locus_tag="ACHOQQ_RS26895"
                     /old_locus_tag="pgaptmp_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          126 a          100 c          118 g          122 t
ORIGIN      
        1 cgcgtatacg gatcgtggat ggcagaaaac gttcaggatc aggtatccat tttaaaccag
       61 ataatgagta agtttgcccc atccatgccc cacgcggtga gatcggatgt cattaaaaaa
      121 gtcaggtcag caggtcagta tgggaaggcg aacaactcgc aggaatgggt gcgctgaagt
      181 tgctggatga taaacatggc gagctgaaat caatgcgtac cgcgccaaat tatttacgtc
      241 ggggtgtcgc cagtctgatt ttacgccaca ttttgcaggt cgcccatgac agatgccttc
      301 atcgcctgag cttagaaacg ggtacacagg ctggatttac ggcctgccat caactttatt
      361 tgaaacatgg attcgttgat tgcgaaccgt ttgctgatta tcgatttgat ccacacagtc
      421 gatttttatc attgacgtta tgcgaaaata atgaattgcc atgagc
//
LOCUS       DAZKFU020000762          465 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.772, whole genome shotgun sequence.
ACCESSION   DAZKFU020000762 DAZKFU020000000
VERSION     DAZKFU020000762.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.772"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>465
                     /locus_tag="ACHOQQ_RS26900"
                     /old_locus_tag="pgaptmp_005380"
     CDS             <1..>465
                     /locus_tag="ACHOQQ_RS26900"
                     /old_locus_tag="pgaptmp_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4473475.1"
                     /translation="NEENPHHLHQPYRLPGQQYDEESGLYYNRNRYYNPLLGRYITQD
                     PIGLAGGWSLYAYPLNPVNGIDPLGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKR
                     LNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNMFGMYGRK"
BASE COUNT          136 a           78 c          131 g          120 t
ORIGIN      
        1 aatgaggaga acccgcatca cctgcaccag ccgtaccggc tgccggggca gcagtatgat
       61 gaggagtcgg ggctgtatta taaccggaac cggtactaca atccgttgct gggacgatat
      121 attactcagg acccgattgg gctggcgggg ggatggagtc tgtatgcgta tccgctgaat
      181 ccggtgaatg gtattgatcc attagggtta agtcctgcag atgtagcgtt aatgcgaaaa
      241 aaagaacaac taaaccatca aagagcatgg gatatattat ctgatactta tgatgatatg
      301 aagagattaa atttaggtgg gactgatcaa tttttccatt gtatggcatt ttgtcgagtg
      361 tctaaattaa atgacgctgg tgttagccga tcagcgaaag ggctgggtta tgaaaaagag
      421 attagagatt acgggttaaa tatgttcggt atgtacggca gaaaa
//
LOCUS       DAZKFU020000763          464 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.773, whole genome shotgun sequence.
ACCESSION   DAZKFU020000763 DAZKFU020000000
VERSION     DAZKFU020000763.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.773"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..148)
                     /locus_tag="ACHOQQ_RS26905"
                     /old_locus_tag="pgaptmp_005381"
     CDS             complement(<1..148)
                     /locus_tag="ACHOQQ_RS26905"
                     /old_locus_tag="pgaptmp_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473476.1"
                     /translation="MKKLLVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATN"
     gene            complement(234..419)
                     /locus_tag="ACHOQQ_RS26910"
                     /old_locus_tag="pgaptmp_005382"
     CDS             complement(234..419)
                     /locus_tag="ACHOQQ_RS26910"
                     /old_locus_tag="pgaptmp_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4473477.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPATVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          112 a          125 c          110 g          117 t
ORIGIN      
        1 cattcgtggc attcacctga aaggtccggg agtgtaattg cgtacattta ccactgaacg
       61 aaccttcaac aagaacacga ccacgctgca aaatacggaa cggaattgtt ccctgaaaag
      121 gttctacggt taccagtaat ttcttcatgc attctccgga taacaaaaat actagttaat
      181 acactgagtg cggatatatt ccttcgcccc ttccagctgc ttctgcattg tcatcaaccg
      241 ttctctgagg atgaaataat cccgttcagc ggtgtctgcc agtcgggggc cggttgcatt
      301 atccacgccg gaggtgctgg tggcttcacg cacggtaccg tggcaggtgg cgttgatccg
      361 caggcgctta cgaccagcgg caacatcagc acgcagagtt tcattttcag ctctcgcatc
      421 ggctaattcc ctcgagtatt ttgcatcgag cgcagcaaca tcga
//
LOCUS       DAZKFU020000764          464 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.774, whole genome shotgun sequence.
ACCESSION   DAZKFU020000764 DAZKFU020000000
VERSION     DAZKFU020000764.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.774"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>464
                     /locus_tag="ACHOQQ_RS26915"
                     /old_locus_tag="pgaptmp_005383"
     CDS             <1..>464
                     /locus_tag="ACHOQQ_RS26915"
                     /old_locus_tag="pgaptmp_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HGU4473478.1"
                     /translation="CSQALTLNLRGGYRSASHAYETLIQVSQGWNNGWWASFESNTWN
                     TIHNNENELFALNYNKIDVNKTYKIDDKWALIPGIQTQWMSAGSQYNPYLKISYQATP
                     DLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKINDSWLFAWQTTV"
BASE COUNT          150 a           82 c          106 g          126 t
ORIGIN      
        1 gtgctctcag gcattaactc ttaatctacg tgggggatac agaagcgcca gtcacgctta
       61 tgaaacgttg attcaggtta gtcaggggtg gaataatggt tggtgggcaa gttttgagtc
      121 aaacacctgg aatacaattc acaacaatga aaatgaattg tttgctctca attataataa
      181 aatagatgta aataaaacat ataaaataga tgacaaatgg gctctaatcc cagggattca
      241 gacacaatgg atgtcagcag gaagtcagta taacccttat ctgaaaattt cgtatcaggc
      301 aacgccagat cttaatctgg ccttacgcta tcgctatgac tggaaagctt tccgacagac
      361 ggatttagat ggcaacaggg cgcgtaatga tcagcatcag tttgatggtt atgtgacata
      421 caaaattaat gattcatggt tgtttgcatg gcagaccacg gtat
//
LOCUS       DAZKFU020000765          461 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.775, whole genome shotgun sequence.
ACCESSION   DAZKFU020000765 DAZKFU020000000
VERSION     DAZKFU020000765.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.775"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>461)
                     /locus_tag="ACHOQQ_RS26920"
                     /old_locus_tag="pgaptmp_005384"
     rRNA            complement(<1..>461)
                     /locus_tag="ACHOQQ_RS26920"
                     /old_locus_tag="pgaptmp_005384"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           84 a          148 c           99 g          130 t
ORIGIN      
        1 ccttctcccg aagttacggc accattttgc ctagttcctt cacccgagtt ctctcaagcg
       61 ccttggtatt ctctacctga ccacctgtgt cggtttgggg tacgatttga tgttacctga
      121 tgcttagagg cttttcctgg aagcagggca tttgttgctt cagcaccgta gtgcctcgtc
      181 atcacgcctc agccttgatt ttccggattt gcctggaaaa ccagcctaca cgcttaaacc
      241 gggacaaccg tcgcccggcc aacatagcct tctccgtccc cccttcgcag taacaccaag
      301 tacaggaata ttaacctgtt tcccatcgac tacgcctttc ggcctcgcct taggggtcga
      361 ctcaccctgc cccgattaac gttggacagg aacccttggt cttccggcga gcgggctttt
      421 cacccgcttt atcgttactt atgtcagcat tcgcacttct g
//
LOCUS       DAZKFU020000766          461 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.776, whole genome shotgun sequence.
ACCESSION   DAZKFU020000766 DAZKFU020000000
VERSION     DAZKFU020000766.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.776"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>461)
                     /locus_tag="ACHOQQ_RS26925"
                     /old_locus_tag="pgaptmp_005385"
     CDS             complement(<1..>461)
                     /locus_tag="ACHOQQ_RS26925"
                     /old_locus_tag="pgaptmp_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HGU4473479.1"
                     /translation="MFTQPGSFEAELFNREGKLWPEADVTLIDLGHLAREGYEAQMAL
                     TMVSMTNMINNIAERDQYLGRDILFVVDEAHIVTVNPLLSPYMTKVVKMWRKLGAWLW
                     LATQNLKDYPDVSEKMLNMAEWWVCLTMPPEEVNNIARFKSLTEEQKAVLLS"
BASE COUNT          105 a          149 c           98 g          109 t
ORIGIN      
        1 gacagcagta ccgccttctg ctcctccgtc agggatttaa aacgggcaat attgttcact
       61 tcctccggcg gcatggtcag gcacacccac cactctgcca tattcagcat cttctccgag
      121 acatccggat aatccttcag gttctgcgtg gccagccaca gccaggcccc cagcttgcgc
      181 cacattttca ccactttggt catatacggg gacaaaagcg ggtttaccgt cacgatatgc
      241 gcctcatcca ccacaaacag aatatcccgt cccagatact gatcgcgctc ggcaatgttg
      301 ttaatcatat tggtcattga caccatggtc agtgccatct gggcttcata accttcacgg
      361 gcaagatgcc ccagatcaat cagggtcaca tccgcttccg gccacagttt tccttcccgg
      421 ttaaacagct ccgcttcaaa actgcccggc tgggtgaaca t
//
LOCUS       DAZKFU020000767          460 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.777, whole genome shotgun sequence.
ACCESSION   DAZKFU020000767 DAZKFU020000000
VERSION     DAZKFU020000767.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.777"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..114)
                     /locus_tag="ACHOQQ_RS26930"
                     /old_locus_tag="pgaptmp_005386"
     CDS             complement(<1..114)
                     /locus_tag="ACHOQQ_RS26930"
                     /old_locus_tag="pgaptmp_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473480.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVN"
     gene            132..323
                     /gene="yfgG"
                     /locus_tag="ACHOQQ_RS26935"
                     /old_locus_tag="pgaptmp_005387"
     CDS             132..323
                     /gene="yfgG"
                     /locus_tag="ACHOQQ_RS26935"
                     /old_locus_tag="pgaptmp_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HGU4473481.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          116 a          116 c           83 g          145 t
ORIGIN      
        1 attaacttcc ataacattaa aatatgtatc cactgacgct tttttacata acgaagaatt
       61 gaccattttg tcctgttgtg ccttaatgta agtaccgtcc acagcgtggg acatacttca
      121 aggaaccttt tgtgagtcag gcaaccagta tgcgaaaacg acaccgattt aacagtcgca
      181 tgacccgtat cgtactgctc atcagcttta tcttcttctt tggccgtttt atctactcgt
      241 ccgtcggtgc ctggcagcac catcagagca aaaaagaagc tcagcaatcc acactctccg
      301 tcgaatcacc ggtacaacgt tagcggttac cttctccact ttcacagaac ataacggcgc
      361 ttcgctgtcg gatgcttttg ctctttggaa ttatcaaagc ggcagatatt ctttcatctt
      421 aaattttacg tctttgtcct gactgatgtt tatcctgttt
//
LOCUS       DAZKFU020000768          459 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.778, whole genome shotgun sequence.
ACCESSION   DAZKFU020000768 DAZKFU020000000
VERSION     DAZKFU020000768.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.778"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>459)
                     /locus_tag="ACHOQQ_RS26940"
                     /old_locus_tag="pgaptmp_005388"
     CDS             complement(<1..>459)
                     /locus_tag="ACHOQQ_RS26940"
                     /old_locus_tag="pgaptmp_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4473482.1"
                     /translation="QYRTTRDVLLQPLHLADARLHTESDGRSAIRLRFECPEKVDWSK
                     AGIDKVAIFLNAEAPVSAALHLAMTRRVHAMYARHAGTYTGRHQFDGWCRPMGFDDND
                     CLWKKADTAFSGYQLLLEYFSFRPKFMFVELRGLDTIGLTAASTWFEIDIV"
BASE COUNT          109 a          126 c          128 g           96 t
ORIGIN      
        1 cacaatgtcg atttcaaacc aggtactggc cgcagtgagc ccgatggtgt cgaggccgcg
       61 cagttcaaca aacataaact tcggacggaa gctgaaatac tccagcagca actggtaacc
      121 actgaaggct gtatcggctt tcttccacag gcagtcattg tcgtcaaagc ccatgggtct
      181 gcaccagccg tcaaactgat gacgtccggt atatgtcccg gcatgacgcg catacatggc
      241 atgtacacgc cgggtcattg ccagatgcag agcagcgctt accggcgcct ctgcattaag
      301 aaaaatggcg actttgtcga tcccggcttt actccagtcc actttttcag ggcattcgaa
      361 gcgcaggcgg atggcagagc ggccatcgga ttcagtatgg agacgggcat ctgcaaggtg
      421 cagcggttgt agcaacacat cgcgggtcgt ccggtactg
//
LOCUS       DAZKFU020000769          456 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.779, whole genome shotgun sequence.
ACCESSION   DAZKFU020000769 DAZKFU020000000
VERSION     DAZKFU020000769.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.779"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(143..280)
                     /locus_tag="ACHOQQ_RS26945"
                     /old_locus_tag="pgaptmp_005389"
     CDS             complement(143..280)
                     /locus_tag="ACHOQQ_RS26945"
                     /old_locus_tag="pgaptmp_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HGU4473483.1"
                     /translation="MEDPVADIAVIFNWPPAEIFAMTPAEVVAWRERAAARSGSHNHD
                     E"
BASE COUNT           99 a          135 c          125 g           97 t
ORIGIN      
        1 ggcttcactc tgccgttcaa gcgagcggat ggagtcctga gtttcacgga gggaatcaga
       61 aagcgcgccc acggtttcgc gggcgcgcct gagaggttgt gtcagggtgt ctgccgccgc
      121 cagagcaacc ctgatatgca gattattcat catgattgtg acttccgctt ctggcagccg
      181 cccgctcacg ccaggcgacc acctctgcag gcgtcattgc aaatatctct gcgggcggcc
      241 agttaaaaat aacagcaata tccgcaacgg gatcttcaat gcagtcaaag caaagacagg
      301 ttacggctct gccgtgttct ccggtgccgg tgcattcgac cccgagggtg cgaaaaaatt
      361 aaccgccgcc gagctcagcg ccgtaaaatc cgccacattc atggcataca tttcggcctc
      421 cttgagtttt ggctctgtca cacgcgcaag ccaggt
//
LOCUS       DAZKFU020000770          455 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.780, whole genome shotgun sequence.
ACCESSION   DAZKFU020000770 DAZKFU020000000
VERSION     DAZKFU020000770.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.780"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..360
                     /gene="ssb"
                     /locus_tag="ACHOQQ_RS26950"
                     /old_locus_tag="pgaptmp_005390"
     CDS             <1..360
                     /gene="ssb"
                     /locus_tag="ACHOQQ_RS26950"
                     /old_locus_tag="pgaptmp_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4473484.1"
                     /translation="IAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYSTEVVVSVNGT
                     LQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGMPPMDFDDDI
                     PFMGRGYGVERRIIHVI"
BASE COUNT          114 a          104 c          130 g          107 t
ORIGIN      
        1 attgccggtg aataccttcg taaaggttca caggtgtata ttgaaggtca gttaagaacc
       61 cggaagtggt tcgatcagca gaatggtgtt gatcgttatt ccacggaagt tgttgtgagt
      121 gtcaatggta ccctgcagat gctggggtct ccgcgtcagc agcagggtgg tggaaatgtt
      181 tcatctgctc ctgctcaggg aggatgggga caaccaccac aaaatgcaca acaatcaacg
      241 ggaaaaaacg gtcaggcttc ctcaggaagc gggggaatgc cgccgatgga ctttgacgat
      301 gatatcccct ttatggggcg tgggtatggc gttgaacgac ggattatcca tgtcatctga
      361 tgttaaccac ggggcttacc gccccacttt taattcaagg aggcggttgt gaatgaaaac
      421 acacccacta cccccacgcc acatgatgcg gcgtt
//
LOCUS       DAZKFU020000771          454 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.781, whole genome shotgun sequence.
ACCESSION   DAZKFU020000771 DAZKFU020000000
VERSION     DAZKFU020000771.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.781"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>454
                     /locus_tag="ACHOQQ_RS26955"
                     /old_locus_tag="pgaptmp_005391"
     CDS             <1..>454
                     /locus_tag="ACHOQQ_RS26955"
                     /old_locus_tag="pgaptmp_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473485.1"
                     /translation="FFALDGFTQKVIERIQRQLDVCEDRDIYLKVAPWFKGKARIKVK
                     GFWLGNNITFLGLSVTGVSLPAGYTINYDRDNSNLVDEKAEQGSRQAWSNVPVRHIKN
                     PDEPFSVDNAFSPDSGASTLEVEASVFEFLDDGCVLSPLRRKKAKYTSA"
BASE COUNT          138 a           76 c          105 g          135 t
ORIGIN      
        1 tttctttgcc cttgatgggt ttacccagaa agtgattgaa agaatacaac gtcaattaga
       61 tgtatgtgaa gatcgtgaca tatacctcaa agtagctccc tggtttaaag gaaaggccag
      121 aattaaagtc aaaggtttct ggctgggaaa taatattacc ttcctggggc tttctgtaac
      181 aggtgtttct ttgccagcag gatatactat aaattatgac agagataaca gtaatctggt
      241 tgatgaaaaa gctgaacaag ggagcaggca agcctggagt aatgttcccg ttcggcatat
      301 aaaaaatccg gatgaacctt tcagtgttga taacgctttt tcgcctgatt ctggagcgtc
      361 gactcttgaa gttgaagcgt cagtttttga gtttcttgac gatggctgtg tattgagtcc
      421 tttaagaaga aaaaaagcaa aatatacatc tgct
//
LOCUS       DAZKFU020000772          453 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.782, whole genome shotgun sequence.
ACCESSION   DAZKFU020000772 DAZKFU020000000
VERSION     DAZKFU020000772.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.782"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>453)
                     /gene="eilA"
                     /locus_tag="ACHOQQ_RS26960"
                     /old_locus_tag="pgaptmp_005392"
     CDS             complement(<1..>453)
                     /gene="eilA"
                     /locus_tag="ACHOQQ_RS26960"
                     /old_locus_tag="pgaptmp_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HGU4473486.1"
                     /translation="AVMYMNGRMEMYRYTPESLKRALVIFRDCVSTQPQNTLPYCCLA
                     ECHISLALLGISEQKQAITTAVTSIEKALEINPSNSQALGLLGLISGLKDEHSVSNVL
                     FKQAHLLKPNSPDVYYYQSLLYFLNGDLARAFNLIEKSIALEPNKMGI"
BASE COUNT          130 a           86 c           86 g          151 t
ORIGIN      
        1 ttatgcccat tttattgggt tcaagagcaa tgcttttttc tattaaatta aatgctcttg
       61 caaggtcgcc gttcaaaaaa tatagcagcg actgataata ataaacgtct ggcgaattag
      121 gtttgagtaa atgtgcttgt ttaaatagca cattagacac tgaatgttca tcttttagcc
      181 cactgattaa acccagtaaa cccaatgcct gagaatttga agggttaatt tccagagcct
      241 tttcaatgga ggttactgcc gtagttattg cttgtttttg ttcactgatt ccaaggagtg
      301 ctaatgatat atggcattcc gccaggcaac agtagggcaa ggtattttgc ggttgcgtac
      361 tcacacaatc tctaaatatt actaatgctc gtttcaaact ttctggtgta taacggtaca
      421 tttccattct gccattcata tacataacag cag
//
LOCUS       DAZKFU020000773          451 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.783, whole genome shotgun sequence.
ACCESSION   DAZKFU020000773 DAZKFU020000000
VERSION     DAZKFU020000773.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.783"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..>451
                     /locus_tag="ACHOQQ_RS26965"
                     /old_locus_tag="pgaptmp_005393"
     CDS             10..>451
                     /locus_tag="ACHOQQ_RS26965"
                     /old_locus_tag="pgaptmp_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4473487.1"
                     /translation="MLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREGEYLPSER
                     ELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGIVKDFLST
                     ADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSL"
BASE COUNT          150 a           94 c           98 g          109 t
ORIGIN      
        1 aaaaaaaccg tgttacccgg gctaacaacg accagaaagc ccattgttac aaaaaaactt
       61 tcagaaatcg ttgccgaaga gcttgaaaga atgatccgcc aggaggagat aagggaaggt
      121 gaatacctgc cttctgaacg tgaacttatg gcattctttg gcgttggaag accatctgtc
      181 agagaggctt tatcatctct cagacataaa gggctaatcc ggtataacaa gggagaaaaa
      241 gccagtgtct gccgtccatc accagaaacc atcattaatg ggttatcagg cattgttaaa
      301 gattttttat ccacagctga tggcatcaag gattttgaaa gactacgcat cttttttgaa
      361 accaaccttg taagatatgc agcaaaaaat gcaacgcctg aggatattca caggcttgag
      421 aaggtgctca gtatccatca taactcactg a
//
LOCUS       DAZKFU020000774          451 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.784, whole genome shotgun sequence.
ACCESSION   DAZKFU020000774 DAZKFU020000000
VERSION     DAZKFU020000774.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.784"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..201
                     /locus_tag="ACHOQQ_RS26970"
                     /old_locus_tag="pgaptmp_005394"
     CDS             <1..201
                     /locus_tag="ACHOQQ_RS26970"
                     /old_locus_tag="pgaptmp_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473488.1"
                     /translation="VKGGMNLNGLPSSTNVIDLRGKDMGTYIDANGACWAPGTPSIIM
                     YPGGSGPSYSMSSSTSSANSGS"
BASE COUNT          139 a           86 c           89 g          137 t
ORIGIN      
        1 gtgaaaggag ggatgaatct taatggatta cctagttcta ctaatgtaat agatctacga
       61 ggaaaagata tgggaacata tattgatgct aatggagcat gctgggctcc gggtactcca
      121 tccatcatca tgtatccggg gggaagtgga ccttcttata gtatgagtag ttccacatcc
      181 agtgcaaaca gcggcagtta aataaatacc ctccggcatg gccggagggt atttatgatg
      241 tgcctgtaag actctacttc tgtcgtctct ttacaaaggc atatgatctg tcattttgta
      301 ccagacatct taacgagagc cttttcatat attacttaca tacgtgaaat ctacagtata
      361 aagcaaaaat tttattaaac ctgttttata tttatcatca atacaacaga tttaattccg
      421 gaaagtcact catcatccct tgaatgaacg t
//
LOCUS       DAZKFU020000775          449 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.785, whole genome shotgun sequence.
ACCESSION   DAZKFU020000775 DAZKFU020000000
VERSION     DAZKFU020000775.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.785"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>449
                     /locus_tag="ACHOQQ_RS26975"
                     /old_locus_tag="pgaptmp_005395"
     CDS             <1..>449
                     /locus_tag="ACHOQQ_RS26975"
                     /old_locus_tag="pgaptmp_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4473489.1"
                     /translation="EGIVITTVRSRGSRKSRFTLTFEGIPYSETVCWRPALLSRPVIS
                     GSLPARIESHEKNDIYAHLDDQGRYRVKLDFDRNSTERGYAYLWLRLAKPCAGDTYGF
                     HSPLIDGTEVAIVFDGGDPDRPYIAYALHDSEHPDHVTSDNHTRNVWR"
BASE COUNT           93 a          122 c          131 g          103 t
ORIGIN      
        1 gaaggcatcg ttatcaccac ggtgcgttcc cgcggttcac gcaaaagtcg ctttacgctg
       61 acgtttgagg ggatccccta cagtgaaacg gtctgctggc gtccggcact gctcagtcgc
      121 ccggtgatat ccggctcgct tccggcgcgg attgagagtc acgagaagaa cgatatctac
      181 gctcatctgg acgatcaggg gcgttaccgg gtgaagctgg actttgaccg caacagtaca
      241 gaacggggtt acgcttatct gtggttgcgt ctggctaaac cctgtgccgg tgatacgtac
      301 ggtttccact caccgcttat cgacggaacg gaagtggcca ttgtatttga cggcggagat
      361 ccggatcgtc cctacatcgc ttatgccctg catgattcag agcatccgga tcatgtcacc
      421 agtgacaatc atacgcggaa cgtgtggcg
//
LOCUS       DAZKFU020000776          449 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.786, whole genome shotgun sequence.
ACCESSION   DAZKFU020000776 DAZKFU020000000
VERSION     DAZKFU020000776.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.786"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          145 a           80 c           74 g          150 t
ORIGIN      
        1 tcgaataaaa caacaaggtt gataagttac gattaattta aatcttatta tttgattaac
       61 aaaacatcag cattactgca tcttattctc tatatctttt acgccgggat agattcttta
      121 tggtcattac gggcaggtac catttcatta tattaccatt tacattcact cttgtaaccc
      181 ttaagagtta tataaaaggc aatagttctt gctcatggta atactgagat cggattaacc
      241 gtgaggttag ctatatagag agagctcttt ggctttgcga caattagctt aagcagccac
      301 attatgtttg gcttaccgtt gaaaggtaga aagaagagca aaatggttca caaggtttca
      361 ttcccatcta atagcaatta cccctataca tcatccctta acgtactctg ggcttcatga
      421 aaatgttatt caaaattaaa gattgtaat
//
LOCUS       DAZKFU020000777          448 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.787, whole genome shotgun sequence.
ACCESSION   DAZKFU020000777 DAZKFU020000000
VERSION     DAZKFU020000777.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.787"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..426)
                     /locus_tag="ACHOQQ_RS26980"
                     /old_locus_tag="pgaptmp_005396"
     CDS             complement(<1..426)
                     /locus_tag="ACHOQQ_RS26980"
                     /old_locus_tag="pgaptmp_005396"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HGU4473490.1"
                     /translation="MFLASLIQPNEDIRILRLRAFLRMIRIGEGTIQEDGYRTMFTGV
                     KFTDFSKHPNIRHEANGVVSTAAGAYQFLYGTWRNLQRRYSFPDFSPSNQDLGCIALI
                     AGRKALDVVMQGKISEAIHLCRIEWASLPGSPHGQPTANK"
BASE COUNT          134 a           97 c           82 g          135 t
ORIGIN      
        1 tttatttgca gttggttgac catgcggtga accgggtaaa ctggcccact caatacgaca
       61 taaatgtatg gcttcactaa tcttaccctg cattactaca tctaatgctt ttcgacctgc
      121 aatgagcgct atacaaccga gatcttgatt gctcggactg aaatctggaa atgaatatct
      181 acgttgcaaa tttctccatg ttccgtataa aaattgataa gctccagcag cagtcgaaac
      241 aacaccatta gcttcatgcc tgatattggg gtgtttacta aaatctgtaa attttacacc
      301 agtaaacatc gttctgtatc cgtcttcctg aattgtccct tcaccgatgc ggatcattcg
      361 tagaaatgca cgtaaacgaa gaattctaat atcttcatta ggttgaatta gggaggctaa
      421 aaacattatt ggatgcatat gccacaac
//
LOCUS       DAZKFU020000778          444 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.788, whole genome shotgun sequence.
ACCESSION   DAZKFU020000778 DAZKFU020000000
VERSION     DAZKFU020000778.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.788"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>444
                     /locus_tag="ACHOQQ_RS26985"
                     /old_locus_tag="pgaptmp_005397"
     CDS             <1..>444
                     /locus_tag="ACHOQQ_RS26985"
                     /old_locus_tag="pgaptmp_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008807102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HGU4473491.1"
                     /translation="FAITDNTVGTRIVRIVTDRPTQNVVTQLFSEQKTFFEERYTEGV
                     EFSGGYITSGDEFFVIPDFDDVIAVLDAINNPTSIPPWEPEEISAFNIIALFSGYPEE
                     DGKPVTALIQSFDKRQVIDNRRTILQKMFQASNTFCQSTEHGIVI"
BASE COUNT          147 a           79 c           84 g          134 t
ORIGIN      
        1 tatttgcaat aacagataat acagttggca cacgtattgt cagaattgtt actgacagac
       61 cgactcaaaa tgtagtcaca cagttattca gtgaacaaaa aacatttttt gaagaacgct
      121 acacagaagg tgtggaattt tcaggtggat acatcactag tggagatgag ttttttgtca
      181 ttcctgactt tgatgatgtt atcgctgtat tagatgccat taataatccc acatctatcc
      241 ctccatggga gccggaagaa atatcagcct tcaatataat cgctcttttt tcaggctatc
      301 ctgaagagga tggaaaacct gtaactgcat taattcaaag cttcgataaa agacaagtta
      361 ttgataacag aaggacaatt ttacaaaaaa tgtttcaggc aagcaatact ttttgccagt
      421 ctactgagca tggtatagtc attg
//
LOCUS       DAZKFU020000779          443 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.789, whole genome shotgun sequence.
ACCESSION   DAZKFU020000779 DAZKFU020000000
VERSION     DAZKFU020000779.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.789"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>443
                     /locus_tag="ACHOQQ_RS26990"
                     /old_locus_tag="pgaptmp_005398"
     CDS             <1..>443
                     /locus_tag="ACHOQQ_RS26990"
                     /old_locus_tag="pgaptmp_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4473492.1"
                     /translation="LYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSR
                     HSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGE
                     RGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWR"
BASE COUNT          107 a          110 c          139 g           87 t
ORIGIN      
        1 gttgtacgcc atagaggcgg atataagggg aatgccggca gagcagcggc ttgctgaacg
       61 tcagcgaaaa acgaaaccgc tgttgaaatc cctggaaagc tggttgcgtg aaaagatgaa
      121 gaccctgtcg cgacactcag agttggcgaa ggcgttcgcg tacgcactta accagtggcc
      181 ggcactgacg tactatgcga acgatggctg ggtggaaatc gacaacaaca tcgctgaaaa
      241 tgccctgcgg gcggtcagtc tgggtcgtaa aaacttcctg ttcttcggct ctgaccatgg
      301 tggtgagcgg ggagcgctac tgtacagcct gatcgggacg tgcaaactga atgacgtgga
      361 tccagaaagc taccttcgcc atgtgcttgg cgtcatagca gactggccgg tcaaccgggt
      421 cagcgaactg cttccgtggc gca
//
LOCUS       DAZKFU020000780          440 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.790, whole genome shotgun sequence.
ACCESSION   DAZKFU020000780 DAZKFU020000000
VERSION     DAZKFU020000780.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.790"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..131)
                     /locus_tag="ACHOQQ_RS26995"
                     /old_locus_tag="pgaptmp_005399"
     CDS             complement(<1..131)
                     /locus_tag="ACHOQQ_RS26995"
                     /old_locus_tag="pgaptmp_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HGU4473493.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQK"
     gene            177..>440
                     /locus_tag="ACHOQQ_RS27000"
                     /old_locus_tag="pgaptmp_005400"
     CDS             177..>440
                     /locus_tag="ACHOQQ_RS27000"
                     /old_locus_tag="pgaptmp_005400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473494.1"
                     /translation="MRITDNKKTNQAEYTFNAAYFLIAVATGNTAHFLTSVATGNTAH
                     FLISVATGNTAHFLISVATGNTAHFLTSVATGNTAHFLITVATG"
BASE COUNT          101 a          123 c           80 g          136 t
ORIGIN      
        1 tccttctgat tattcctgcg tccctcccag agacggatcg catatcgggt atgaagggca
       61 atagtcagcg atgactgcag agcaccaact tgttctgatt ttctgacttc tccggttttc
      121 tcctggggca ttgtgtttac tccgaatgat ttcatgacca gaagatagtc cgcaggttgc
      181 gtattaccga caacaaaaaa acaaatcagg cagaatatac atttaacgcc gcttattttt
      241 taatcgctgt tgccactggc aacaccgctc actttttaac ctctgttgcc actggcaaca
      301 ccgctcactt tttaatctct gttgccactg gcaacaccgc tcatttttta atctctgttg
      361 ccactggcaa caccgctcac tttttaacct ctgttgccac tggcaacacc gctcattttt
      421 taatcactgt tgccactggc
//
LOCUS       DAZKFU020000781          438 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.791, whole genome shotgun sequence.
ACCESSION   DAZKFU020000781 DAZKFU020000000
VERSION     DAZKFU020000781.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.791"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            37..>438
                     /locus_tag="ACHOQQ_RS27005"
                     /old_locus_tag="pgaptmp_005401"
     CDS             37..>438
                     /locus_tag="ACHOQQ_RS27005"
                     /old_locus_tag="pgaptmp_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HGU4473495.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNP"
BASE COUNT          171 a           86 c           78 g          103 t
ORIGIN      
        1 tactttcagc cgttggattg gtatataata aaagccatgc tctggactga aaacgatgcg
       61 gaaaatactt ctcagtggaa tggttatcca ttacaaattg gtcgttttcg gaaagataaa
      121 gcgatgccag ctttaatctc tggagaaaaa tctacagccc ttgttacccc accacaatgg
      181 agaaacaaag cattcaatgg gttaaaagat ccagaaagaa attactgggc aaaagaacaa
      241 ataactggct ctccagaaga gaatatcaaa gcagcaatta cttacttaat gatgaaactt
      301 tctaatacaa aggaagaatc aactatcgat caatatgact caactctata tagcgccatt
      361 gtccaaaaag gcgatttggc tgacaacatc agaaaagaga gaaaaacgac catacctaac
      421 ttaacgaaaa acaatcct
//
LOCUS       DAZKFU020000782          435 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.792, whole genome shotgun sequence.
ACCESSION   DAZKFU020000782 DAZKFU020000000
VERSION     DAZKFU020000782.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.792"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>435)
                     /locus_tag="ACHOQQ_RS27010"
                     /old_locus_tag="pgaptmp_005402"
     CDS             complement(<1..>435)
                     /locus_tag="ACHOQQ_RS27010"
                     /old_locus_tag="pgaptmp_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU4473496.1"
                     /translation="DMLRARVTDAFGNALAGQTVSVMADNGAAVASTMTTKPDGTVEI
                     SVTSQTAGISVVTASINSSSQSRDVTFIADIRTAQIADLVVIKDGSVADGAMANTLWV
                     RVTDAFGNALAGQTVSVLADNGATTAPTVTTQPDGTVEISVT"
BASE COUNT           69 a          157 c          115 g           94 t
ORIGIN      
        1 ctggtgacac tgatttccac cgtgccgtcc ggctgcgtgg tgaccgtcgg agctgtcgtg
       61 gcaccgttgt ccgccaacac gctgaccgtc tgcccggcaa gcgcattacc gaacgcatcc
      121 gtgaccctca cccacagcgt gttcgccatt gcgccgtctg ccactgagcc gtccttaatg
      181 accaccaggt ccgcgatctg cgccgtcctg atatcggcga taaacgtcac atcccggctt
      241 tgactgctgc tgttgatgct ggcggtgacc acactgattc ctgccgtctg gctggtgaca
      301 ctgatctcca ccgtgccgtc cggctttgtg gtcatcgtcg aagcgaccgc tgcaccgttg
      361 tccgccatca cgctgaccgt ctgcccggca agcgcattac caaacgcatc ggtgaccctc
      421 gcccgcagca tatcc
//
LOCUS       DAZKFU020000783          433 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.793, whole genome shotgun sequence.
ACCESSION   DAZKFU020000783 DAZKFU020000000
VERSION     DAZKFU020000783.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.793"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..288)
                     /locus_tag="ACHOQQ_RS27015"
                     /old_locus_tag="pgaptmp_005403"
                     /pseudo
     CDS             complement(1..288)
                     /locus_tag="ACHOQQ_RS27015"
                     /old_locus_tag="pgaptmp_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT          133 a           88 c           79 g          133 t
ORIGIN      
        1 caggccaagc gtatgctggt tgatcaaata atatccccca agcgcagcaa tattaatatt
       61 cacctgaaat gggtcgatat ctggcttaaa aatattgtgt ttcttccctt catccaataa
      121 ggattccatt aactgcaagt gcgcatgggt aatttctaag agacgctgtg attttgcata
      181 atgcacacct ttactttgat tttcactatg cacaattttt aaaaaccagg gattagcaat
      241 ataataatcc catgtaaaat caataatttt ctcaatggct ttttgtggct ggctaacctg
      301 ctgattaacg aatacaccga taccgacagc gtagaataat tacataccgc acaacaagaa
      361 tgtacggtgt aaggcgtgga agtcattttc tccccgggtg catcgttata tccataatga
      421 gcgatttttg ata
//
LOCUS       DAZKFU020000784          432 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.794, whole genome shotgun sequence.
ACCESSION   DAZKFU020000784 DAZKFU020000000
VERSION     DAZKFU020000784.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.794"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>432)
                     /locus_tag="ACHOQQ_RS27020"
                     /old_locus_tag="pgaptmp_005404"
     CDS             complement(<1..>432)
                     /locus_tag="ACHOQQ_RS27020"
                     /old_locus_tag="pgaptmp_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU4473497.1"
                     /translation="AGVSAVTASINNSSLSRDVTFIADVRTAQIADLVVIKDGVVADG
                     AMANMLRVRVTDANGNTLGGQTVSVTADNSAMVASTVITGPDGTVELSVTSQTAGTST
                     VTASINNSSLSRNVTFIADIRTAQIADLVVIKDDSVADGAMA"
BASE COUNT           68 a          141 c          119 g          104 t
ORIGIN      
        1 cgccattgca ccgtctgcca ctgagtcgtc cttaatgacc accaggtccg cgatctgtgc
       61 cgtcctgata tcggcgataa atgtcacgtt ccggctcagg ctgctgttgt tgatgctggc
      121 ggtgactgta ctggttccgg cggtctggct ggtgactgag agctccaccg tgccgtccgg
      181 ccctgtgatg acagtcgaag cgaccattgc actgttgtct gccgtcacgc tgaccgtctg
      241 tccgccaagc gtattaccgt tagcatcggt gaccctcacc cgcagcatgt tcgccattgc
      301 gccgtctgcc actacgccgt ccttaatgac caccagatca gcgatctgcg ccgtcctgac
      361 atcggcgata aatgtcacgt cccggctcag gctgctgttg ttgatgctgg cggtgaccgc
      421 actgactccc gc
//
LOCUS       DAZKFU020000785          431 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.795, whole genome shotgun sequence.
ACCESSION   DAZKFU020000785 DAZKFU020000000
VERSION     DAZKFU020000785.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.795"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>431
                     /locus_tag="ACHOQQ_RS27025"
                     /old_locus_tag="pgaptmp_005405"
     CDS             <1..>431
                     /locus_tag="ACHOQQ_RS27025"
                     /old_locus_tag="pgaptmp_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473498.1"
                     /translation="DFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQPIN
                     STFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESISSTP
                     LTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIIT"
BASE COUNT          158 a          101 c           72 g          100 t
ORIGIN      
        1 ttgactttac cccaggtatg ataaatacat atcacggcga tatttataac cgcacaacag
       61 ataccgacaa tgttaaaaca ccagacacac cgatgtggcc ttgtgataat cgggaagaac
      121 aacaacccat taattccaca ttctctggag aaggatacaa ccctgaacaa tatgatttag
      181 cgcggcaaca cctcccacaa atttatgcgt gccgcacgaa caccaaatat acagatgcag
      241 actactcaaa agttgtggct caacttgtaa gtcttattac taacattgaa tcgataagtt
      301 cgacaccact tacgcggcag gcacaggaaa tacttaatca aattaataac attagatacg
      361 aaaagaacaa aagtgctgaa tgtcgaatca ttgttgtcgc taatcctaaa cccgacaagg
      421 caatcataac t
//
LOCUS       DAZKFU020000786          429 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.796, whole genome shotgun sequence.
ACCESSION   DAZKFU020000786 DAZKFU020000000
VERSION     DAZKFU020000786.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.796"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(105..>429)
                     /locus_tag="ACHOQQ_RS27030"
                     /old_locus_tag="pgaptmp_005406"
     CDS             complement(105..>429)
                     /locus_tag="ACHOQQ_RS27030"
                     /old_locus_tag="pgaptmp_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HGU4473499.1"
                     /translation="SPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGL
                     ALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTL
                     LEKRN"
BASE COUNT          126 a          110 c          108 g           85 t
ORIGIN      
        1 tgcgccagtg actatatgtg gcttaacaca tcaaggagcg caacaggaag atgaatcaat
       61 atatttaaaa aaggcctgac attgcgccag gccttctgcg ttaattaatt acgtttttcc
      121 agcagggtcc ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag
      181 aaccacagtt gttctaatgc ccagccgccc tggaagatgg caaccgcggt gctgcgcgcc
      241 gggttaacag aagtgttagt caccggaata ctgattaagt gaatcagggt taaggccaga
      301 ccaatagcga tcggcgcaaa acctgccgga gcgaatttgt cggttgcgcc gtggatcacc
      361 aacaggaaac ctgcactcaa taccagttca actaccagcg cggaaagcat ggaataaccg
      421 cctggtgaa
//
LOCUS       DAZKFU020000787          427 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.797, whole genome shotgun sequence.
ACCESSION   DAZKFU020000787 DAZKFU020000000
VERSION     DAZKFU020000787.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.797"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(67..181)
                     /locus_tag="ACHOQQ_RS27035"
                     /old_locus_tag="pgaptmp_005407"
                     /pseudo
     CDS             complement(67..181)
                     /locus_tag="ACHOQQ_RS27035"
                     /old_locus_tag="pgaptmp_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          112 a           84 c          105 g          126 t
ORIGIN      
        1 attcatcatc tttgccaaag aaaaatgttc agaaaataat ccatggaaaa ttgtccggag
       61 cgcttactat tttaatggat tgttagtctt tgcatgagca agcggactga tacatttctc
      121 tttgttctca ttcagaaaat ctcatcagtc ggcgttcaga ccggtgttgt gctttatgga
      181 tgcgttacgc tcctgatgac gtcatttgac gttcaacaac atcacggggc cgcacgacat
      241 ttcacgtcag ttagtgctat cgctcaggaa caaattttcc cgaattggga tatgcccgca
      301 aattgctggt gatgagggga gaatctggtt gagttcggga gaattgattt ggaggcagaa
      361 cgcttaaatc gtggcgtcct gaaacgaaaa acggacctcc gtggaggtcc gtttatatga
      421 atttggt
//
LOCUS       DAZKFU020000788          427 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.798, whole genome shotgun sequence.
ACCESSION   DAZKFU020000788 DAZKFU020000000
VERSION     DAZKFU020000788.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.798"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(191..>427)
                     /locus_tag="ACHOQQ_RS27040"
                     /old_locus_tag="pgaptmp_005408"
     CDS             complement(191..>427)
                     /locus_tag="ACHOQQ_RS27040"
                     /old_locus_tag="pgaptmp_005408"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4473500.1"
                     /translation="ASVSSSVTSLRRPMFLKFPIPVPPSEEQARIVEILDKFDTLTNS
                     ITEGLPREIELRQKQYEYYRDLLFSFPKPETVSN"
BASE COUNT          106 a           73 c          123 g          125 t
ORIGIN      
        1 tcagccttga tgtacttatc aagaacgata aagttactgg tttcagcaat ggtgtgtgtc
       61 tgaagaatca tagcgcatcc tttgtgccgt ctggcggggc cggaagggag ttaagggtga
      121 cttccggcgt gtaaaaatgg tttatatact gaccggatgt taaggtggcc cggtcggtag
      181 caattgtcaa ttagttactg acggtttcag gtttcgggaa actgaacagt aaatcacgat
      241 agtactcgta ttgtttctgg cgcaactcga tttcacgcgg gagaccttcg gtgatggagt
      301 tggtcagggt gtcgaattta tcgagtattt cgacaatgcg agcttgttct tctgaaggtg
      361 gtacaggtat tgggaatttt aaaaacattg gtcgacgaag tgaggtaacc gatgaactta
      421 ctgatgc
//
LOCUS       DAZKFU020000789          425 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.799, whole genome shotgun sequence.
ACCESSION   DAZKFU020000789 DAZKFU020000000
VERSION     DAZKFU020000789.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.799"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>425)
                     /locus_tag="ACHOQQ_RS27045"
                     /old_locus_tag="pgaptmp_005409"
     CDS             complement(<1..>425)
                     /locus_tag="ACHOQQ_RS27045"
                     /old_locus_tag="pgaptmp_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4473501.1"
                     /translation="QRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEAL
                     MRWQREDGKNISPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAA
                     EHLAHPDFVADVLHIKNEISDKFNIVLEITERNLVEDTD"
BASE COUNT          123 a           97 c           72 g          133 t
ORIGIN      
        1 atcagtatct tcaaccagat tgcgttcggt aatctcaaga acaatgttaa atttatcaga
       61 tatttcattt ttaatatgta aaacatcagc aacaaaatca ggatgtgcaa gatgctccgc
      121 ggcaatattt atcccaaggt gaaacggctt tttcactttc cagctttgta catcctgaat
      181 aatcagttca aagagatgct tagttaacgg tataattaaa ttttcctctt ctgcagcgcg
      241 gataaaaact accggagata tatttttacc atcttcgcgt tgccaacgca ttaacgcctc
      301 aacccccaga cacgctttgg ttgtcgtgtc acatactggt tgataatgca cagaaaattc
      361 tccggatgac attcctttct taatttcttc tgccagggac attttccgag atatccagcg
      421 ctgcc
//
LOCUS       DAZKFU020000790          425 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.800, whole genome shotgun sequence.
ACCESSION   DAZKFU020000790 DAZKFU020000000
VERSION     DAZKFU020000790.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.800"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..207
                     /locus_tag="ACHOQQ_RS27050"
                     /old_locus_tag="pgaptmp_005410"
     CDS             <1..207
                     /locus_tag="ACHOQQ_RS27050"
                     /old_locus_tag="pgaptmp_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4473502.1"
                     /translation="APKARTPTAASTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVG
                     SRVVSQEISTADEGDGGQVVVIGR"
BASE COUNT          100 a           93 c          142 g           90 t
ORIGIN      
        1 gcaccgaaag ccaggacgcc cacggcagcc agtacagata acggcaaaca gaacacgtat
       61 ttctcgtcac tggataacat ggttgcccag ggcaatgttc tgcctgttct gtacggtgaa
      121 atgcgtgtgg ggtctcgtgt ggtttctcag gagatcagca cggcagatga aggggacggt
      181 ggtcaggttg tggtgattgg ccgctgatgc aaaatgtttt atgtgaaacc gcctgcgggc
      241 ggttttgtcg tttatggagc gtgacgaatg ggtaaaggca gcagtaaggg gcataccccg
      301 cgcgaagcga aggacaacct gaagtccacg cagctgctga gtgtgatcga tgccatcagc
      361 gaagggccgg ttgaaggtcc ggtggatgga ttaaaaagcg tgctgctgaa cagtacgccg
      421 gtgct
//
LOCUS       DAZKFU020000791          424 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.801, whole genome shotgun sequence.
ACCESSION   DAZKFU020000791 DAZKFU020000000
VERSION     DAZKFU020000791.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.801"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          149 a           81 c           74 g          120 t
ORIGIN      
        1 tagatgttat gcagtacgca caaaaaaaac aggctggaat tgcttgctga tttataatct
       61 ttatctaaat tactattggc gctttggata ataacataag catatattac ctcctagcaa
      121 taatagagat aacctcatat caaggtgtca ctggtaaata atcggataaa agacagtatc
      181 ggtcggcgca tagagcttct tctatttcac cagaagtgca cagcaggaac taccaatcac
      241 acggttagtg tcatgggtgg aggatgcggc ccgttctttc cctataacag ggttacgcag
      301 tttcaatcag tacatgcaga aaaaccccta attcacatca ctgcagaaag catttaatag
      361 aaatatacat cgtttaagaa tttcaattaa atcaagatta ttcttaaaaa tcagataatt
      421 caca
//
LOCUS       DAZKFU020000792          422 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.802, whole genome shotgun sequence.
ACCESSION   DAZKFU020000792 DAZKFU020000000
VERSION     DAZKFU020000792.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.802"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>422
                     /locus_tag="ACHOQQ_RS27055"
                     /old_locus_tag="pgaptmp_005411"
     CDS             <1..>422
                     /locus_tag="ACHOQQ_RS27055"
                     /old_locus_tag="pgaptmp_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4473503.1"
                     /translation="LEQLSDKPESLSMRITATNGQLPRRALESTLLDRVVKTGKVPVR
                     VLNLTAPTMPLYPPANDRFHWRVMSHLGSNFLSMMDNPEVLRGTLALYDWTDDEMNRR
                     RLEAIVAVKHTLIRRFEKGFMLRGVDIEVTLNMDNFAG"
BASE COUNT           92 a          120 c          125 g           85 t
ORIGIN      
        1 ctggagcagt tgtctgataa accggagtcc ctctcgatgc gaatcaccgc gaccaacggg
       61 cagcttccgc gcagggcgct ggaaagtacg ctcctggaca gggtggtcaa aaccggcaaa
      121 gtccccgtca gagtcctgaa cctgaccgca ccgacgatgc cactgtatcc cccggcaaat
      181 gaccggtttc actggcgggt gatgagtcac ctcggttcaa actttctcag catgatggac
      241 aatccggagg tgctgcgggg gacgctggcg ctctatgact ggaccgacga tgaaatgaac
      301 cgccgccggc ttgaagccat cgttgccgtg aagcacaccc tgatccgccg ttttgaaaag
      361 ggctttatgc tccgtggcgt ggacattgag gtcacgctga atatggataa ttttgccggc
      421 ga
//
LOCUS       DAZKFU020000793          422 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.803, whole genome shotgun sequence.
ACCESSION   DAZKFU020000793 DAZKFU020000000
VERSION     DAZKFU020000793.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.803"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>422)
                     /locus_tag="ACHOQQ_RS27060"
                     /old_locus_tag="pgaptmp_005412"
     CDS             complement(<1..>422)
                     /locus_tag="ACHOQQ_RS27060"
                     /old_locus_tag="pgaptmp_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473504.1"
                     /translation="IINYFEQLQNARGYVTRCYMLLAVFIYLIRRNKLHKALRFYDES
                     QKYELPFGYLPGAFSVLRIALEIKVNREKIKHGSLFELLDYVKAYQDAFMDLRVVTDP
                     AYNEDEIQYDANNFTLMRVIKMYNSMLANISTKSDIQPP"
BASE COUNT          137 a           88 c           71 g          126 t
ORIGIN      
        1 ggaggctgaa tatctgactt tgtactaata tttgcaagca tcgaattata cattttaatg
       61 acacgcatca gagtaaaatt attcgcatca tattgtattt catcttcatt atacgcagga
      121 tccgtcacga cccttaagtc cataaatgca tcctgataag cttttacata atcaagtaat
      181 tcgaacagtg aaccatgctt tattttctcc ctgtttactt tgatctccag cgctatcctg
      241 agaactgaaa aagcgccggg aagataccca aaaggtaact cgtatttttg ggattcatca
      301 taaaaccgaa gagctttgtg aagtttgttc cgccgaatca gataaataaa aacggccagt
      361 aacatgtagc acctggtcac ataaccacgg gcattctgca actgttcaaa ataattaata
      421 at
//
LOCUS       DAZKFU020000794          420 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.804, whole genome shotgun sequence.
ACCESSION   DAZKFU020000794 DAZKFU020000000
VERSION     DAZKFU020000794.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.804"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..365)
                     /locus_tag="ACHOQQ_RS27065"
                     /old_locus_tag="pgaptmp_005413"
     CDS             complement(<1..365)
                     /locus_tag="ACHOQQ_RS27065"
                     /old_locus_tag="pgaptmp_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473505.1"
                     /translation="MAELSDFLPYVRRQISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELL"
BASE COUNT          112 a          124 c          101 g           83 t
ORIGIN      
        1 aggagttcat ccggtatctg gctgcgtccg gctttcggag cgacggcata aagcacgctc
       61 acctcaccgg gagaacaggc aaatcgcagg gagcgtccgg agctgatatc cacatcccga
      121 ccgacaaaaa gctcatgatt atcgtcagag atacggatga tatgaacgca ctcctcatca
      181 tctttgtcat acggaagcac gatttctttt cctgctactg gtacaacagt aacctcccga
      241 cggcacacca gcgactgacg gctgaatgcc acggcagcca ttgacagagc atccgtcatc
      301 ataatgttca gtggaccgct gatctgacga cggacatacg gtaaaaaatc actcagttcc
      361 gccatgctgt tcagtctccg caacacgacg gcgaaatgcc tcacgcaccc ggatacggaa
//
LOCUS       DAZKFU020000795          420 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.805, whole genome shotgun sequence.
ACCESSION   DAZKFU020000795 DAZKFU020000000
VERSION     DAZKFU020000795.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.805"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>420)
                     /locus_tag="ACHOQQ_RS27070"
                     /old_locus_tag="pgaptmp_005414"
     CDS             complement(<1..>420)
                     /locus_tag="ACHOQQ_RS27070"
                     /old_locus_tag="pgaptmp_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473506.1"
                     /translation="YNNGYPTTYGNIIHLKGMTAVGEGELLIGWSGTSGAHAPAFIRS
                     RRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPK
                     LAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIK"
BASE COUNT           99 a          113 c          110 g           98 t
ORIGIN      
        1 atttaatgcc gtcctgttcc tgagacaata cggcccgacc actggcaggt ttgcccttaa
       61 tcgtccagcc acgcatatca gggatcacgc ctgacggata agcggctgca agtttcgggt
      121 atgcagattt gtcaaaagtc tgcccctgca tcagggcata accagacgga acggtatctg
      181 atggccacgg gattggtgca ccgactggat aaaactctgc aggaggatga gccgaggtgt
      241 aaagctgcgc ccacggcgac cagtttgcgt cggtcgtatc ccgtcgtgaa cgaataaatg
      301 ccggagcatg agcaccgctt gtaccactcc agccgatgag taactcacct tcgccaacgg
      361 ctgtcatccc tttcaggtga atgatatttc catacgttgt tggatatccg ttgttataca
//
LOCUS       DAZKFU020000796          419 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.806, whole genome shotgun sequence.
ACCESSION   DAZKFU020000796 DAZKFU020000000
VERSION     DAZKFU020000796.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.806"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..98)
                     /locus_tag="ACHOQQ_RS27075"
                     /old_locus_tag="pgaptmp_005415"
     CDS             complement(<1..98)
                     /locus_tag="ACHOQQ_RS27075"
                     /old_locus_tag="pgaptmp_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473507.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTV"
     gene            complement(354..>419)
                     /locus_tag="ACHOQQ_RS27080"
                     /old_locus_tag="pgaptmp_005416"
     CDS             complement(354..>419)
                     /locus_tag="ACHOQQ_RS27080"
                     /old_locus_tag="pgaptmp_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HGU4473508.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          124 a           86 c           84 g          125 t
ORIGIN      
        1 accgtccaat aatcaatatt tctattgtga ttgttaacca ctgcgtgcat tggtgggatt
       61 gaagggtgaa aaatatgtgc aattgggaag cctttcattg atgcctgcct tgtcatcatg
      121 acaaacaata atagatgctt attttcctcg cagggttctt tcgcactatc aaaaaacgct
      181 catttacccg gcgttacagt aaacgaagca acattctgtg gcctacgccg atcaaaagct
      241 ctgtgaaaat ttcccttgtt gtctttgtct gaccttatcg cggcgatcag caattattcg
      301 ttttgtgatc ctcaacacaa aatgctattc gtcgcctatg aagggagaag aatttatgga
      361 aaacgattgc attcacaaaa tgacgtcctg tcagaagaga aaaagttgac gttaatcaa
//
LOCUS       DAZKFU020000797          413 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.807, whole genome shotgun sequence.
ACCESSION   DAZKFU020000797 DAZKFU020000000
VERSION     DAZKFU020000797.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.807"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..366
                     /locus_tag="ACHOQQ_RS27085"
                     /old_locus_tag="pgaptmp_005417"
                     /pseudo
     CDS             1..366
                     /locus_tag="ACHOQQ_RS27085"
                     /old_locus_tag="pgaptmp_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          138 a           88 c           85 g          102 t
ORIGIN      
        1 catctctcaa cgctattaca tctctttttt gaacgtgagg atatttgcca aaagcttaat
       61 attattgacc aaaacaatat caccggttgg gaagtgtggt tccaggtaga gtttgctaat
      121 atgctctgct caacagacca tgaatggtgg cgtgaacaag cactttcctg tgatatgcgc
      181 aaaaaacctg agcgcccgac actacgcaca gatttcttac tacgtaaaaa aggctgggca
      241 caagatagct atatcacgct tgaaataaaa caaaacagag acgcgaccag ttgtgttaaa
      301 aatatgatcg cagacttaga aaaaagcgca aaaataaaac gttccgaact cgatttacgc
      361 agtttctgaa ccttgggtat cacccctatg atagacaggg aaaggctgga ctc
//
LOCUS       DAZKFU020000798          409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.808, whole genome shotgun sequence.
ACCESSION   DAZKFU020000798 DAZKFU020000000
VERSION     DAZKFU020000798.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.808"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>409)
                     /locus_tag="ACHOQQ_RS27090"
                     /old_locus_tag="pgaptmp_005418"
     CDS             complement(<1..>409)
                     /locus_tag="ACHOQQ_RS27090"
                     /old_locus_tag="pgaptmp_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HGU4473509.1"
                     /translation="ESGLGRPQVTALLALRQDAERVWGQFAVAADTDAEFDRVFGESC
                     RKFNSPELWSLEMFRDELIGDLLQALPHPSLDYDRWLLELDPKERNRRHHFGEPEPVV
                     VPPSVRGSSTVQPGAPVSGIRSEVPECQPDTDTE"
BASE COUNT           89 a          135 c          103 g           82 t
ORIGIN      
        1 cctctgtatc cgtatccggt tgacactccg gtacttcaga ccggatcccg gataccggtg
       61 ccccgggttg taccgtactg ctgccacgga ctgaaggagg caccaccacc ggttccggct
      121 caccaaaatg atgacgacgg ttccgctctt tcggatccag ttccagcaac caccggtcat
      181 agtccaggga gggatgtggc agggcctgca acaaatctcc gataagctca tcgcggaaca
      241 tctccagtga ccacagttcc ggcgaattaa acttccggca gctttccccg aacacgcggt
      301 caaattccgc atccgtatcg gctgcaacag caaactgccc ccatacacgt tctgcgtcct
      361 gacgaagtgc gaggagtgct gtaacctgtg gacgccccag tcctgactc
//
LOCUS       DAZKFU020000799          409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.809, whole genome shotgun sequence.
ACCESSION   DAZKFU020000799 DAZKFU020000000
VERSION     DAZKFU020000799.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.809"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..403
                     /locus_tag="ACHOQQ_RS27095"
                     /old_locus_tag="pgaptmp_005419"
     CDS             <1..403
                     /locus_tag="ACHOQQ_RS27095"
                     /old_locus_tag="pgaptmp_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HGU4473510.1"
                     /translation="YINNWNLFPLICLLSGCHFYRERFAERGFFYKVPAVLRDYLSAI
                     PVEINEKARYKPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHIQLPL
                     PLASTLLERITFYAKKNRDELDKISCKWCCD"
BASE COUNT          131 a           72 c           73 g          133 t
ORIGIN      
        1 ctacataaac aattggaatt tatttccatt gatctgttta ctcagtggtt gtcactttta
       61 tcgagaaagg tttgcagaac gcggattttt ttataaggtt cctgctgtgc taagggatta
      121 tttatcagct ataccggtgg aaataaatga aaaggcacga tacaaacccg gcatcgctaa
      181 ctatcacaat atcatcacat gtggtttttc aacgttgtta ccctatataa ggcaacaacc
      241 attggcaatg cagcaacgat ttaatttatt atttcctgat tttgttgacc acatacaatt
      301 gccgttacca cttgcgtcaa ctcttttaga gagaattaca ttttatgcga aaaaaaatag
      361 agatgagctt gataaaatct cctgcaaatg gtgttgtgat taaaagaaa
//
LOCUS       DAZKFU020000800          409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.810, whole genome shotgun sequence.
ACCESSION   DAZKFU020000800 DAZKFU020000000
VERSION     DAZKFU020000800.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.810"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>409)
                     /locus_tag="ACHOQQ_RS27100"
                     /old_locus_tag="pgaptmp_005420"
     CDS             complement(<1..>409)
                     /locus_tag="ACHOQQ_RS27100"
                     /old_locus_tag="pgaptmp_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGU4473511.1"
                     /translation="VLVIGLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTY
                     KLQQTNSSYRYANGTQGSALDLILENPVIGYGYGNVAYKDVYNKRVVDYPEWTFRQSI
                     GPHNFALFIWFGTGLLGLVSLMMLYCAILKECIK"
BASE COUNT          136 a           84 c           62 g          127 t
ORIGIN      
        1 ttttatacac tctttcaata ttgcacagta tagcatcata agacttacca gccctaataa
       61 accagtgcca aaccagatga atagcgcaaa attatgtggc cctattgatt gtctaaaggt
      121 ccattctgga taatcaacga cacgtttatt atagacatct ttatatgcaa cgttaccgta
      181 accataacca ataacaggat tttctaatat tagatcgagt gcgctgcctt gagtaccatt
      241 tgcatagcga taagaactat tagtttgttg aagtttatac gttagctttg cggtcatctc
      301 cttatgtgtg aaaataaccg acaatgcaat gatggaaacc attactccta ctaatagtaa
      361 cttccattgt ttaaacatca gaatccatat taatccaatg actaacacg
//
LOCUS       DAZKFU020000801          408 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.811, whole genome shotgun sequence.
ACCESSION   DAZKFU020000801 DAZKFU020000000
VERSION     DAZKFU020000801.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.811"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..>408
                     /locus_tag="ACHOQQ_RS27105"
                     /old_locus_tag="pgaptmp_005421"
     CDS             10..>408
                     /locus_tag="ACHOQQ_RS27105"
                     /old_locus_tag="pgaptmp_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HGU4473512.1"
                     /translation="MVDNVTVGGVCAQSPSFMPDLDGGKNKAQLIIDDIVAYLKNPSV
                     YSLEKEGPLNHFVNNCSEIEPWVLIGGGLIIIVSTSEQDPEGLIVTVGDIEGKCATQI
                     PVPPALTTSLKDIFIRPHTNAKYESFTKEQI"
BASE COUNT          125 a           69 c           89 g          125 t
ORIGIN      
        1 ggaaagaaaa tggttgataa cgtaactgtt ggcggtgtat gtgcacaatc tccttctttc
       61 atgcctgatt tggatggagg aaaaaataaa gctcaattga tcattgatga tatagtggcg
      121 tatcttaaaa atccttcagt ttattcgctt gaaaaagaag gtccgttaaa tcattttgtt
      181 aataattgta gcgagattga accatgggtt ttaatcggtg gtggacttat tattattgtc
      241 tccacatctg agcaagaccc tgaaggtctg attgtaactg tgggggatat tgaaggaaag
      301 tgtgctacac agattcccgt accacctgcg cttacaacat ccctcaaaga tatttttatt
      361 cgccctcata cgaacgcaaa gtatgagagt tttacaaaag agcagata
//
LOCUS       DAZKFU020000802          401 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.812, whole genome shotgun sequence.
ACCESSION   DAZKFU020000802 DAZKFU020000000
VERSION     DAZKFU020000802.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.812"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..197)
                     /locus_tag="ACHOQQ_RS27110"
                     /old_locus_tag="pgaptmp_005422"
     CDS             complement(<1..197)
                     /locus_tag="ACHOQQ_RS27110"
                     /old_locus_tag="pgaptmp_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HGU4473513.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKVHLPEADCLVEPFVGGASV
                     FMNTSYKRYILGDSNGALINF"
     gene            complement(222..>401)
                     /locus_tag="ACHOQQ_RS27115"
                     /old_locus_tag="pgaptmp_005423"
     CDS             complement(222..>401)
                     /locus_tag="ACHOQQ_RS27115"
                     /old_locus_tag="pgaptmp_005423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473514.1"
                     /translation="VLLPPPERGKKSPRIAAFIPQRCAEFFTHKAPQLPGDKNRFSLA
                     ITQQEQREVMGFINA"
BASE COUNT           92 a          104 c           98 g          107 t
ORIGIN      
        1 cagaaattaa tcagcgcacc attggaatca cccaggatgt agcgtttgta actggtgttc
       61 atgaataccg atgcgccacc cacgaacggt tcaaccaggc agtcagcttc tggcagatgc
      121 accttcagtg tgttcatgat gcggcttttg ccacccaccc atttcagcgg tgtgccggat
      181 aacagttctt tgctcataac gcggcctctt tgttataggt ttcatgcgtt aataaatccc
      241 atgacttccc gttgttcttg ctgagtaatc gccagcgaaa accgattctt atcaccaggt
      301 agttgtgggg ctttatgcgt gaaaaattcc gcacaccgtt gcggaataaa cgcagcgatg
      361 cgcgggcttt tctttccacg ttcagggggg ggcagcaaca c
//
LOCUS       DAZKFU020000803          399 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.813, whole genome shotgun sequence.
ACCESSION   DAZKFU020000803 DAZKFU020000000
VERSION     DAZKFU020000803.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.813"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          117 a           76 c           99 g          107 t
ORIGIN      
        1 gaaaaaaatt ttacttccgc atcatgcggc gaatgtggga attgcccagg cggcggggga
       61 taggggctgc agacagttat ccactattcc tgtggataac catgtgtatt agagttagaa
      121 aacacgaggc aagcgagaga atacgcggct tgcacgcgaa ttggcgttaa agacggctca
      181 aagaaatttc ttttattttt taactggtta gataattgca atggcagtca ctaacgaggc
      241 atctcttgcc ataaaactgt caccactcat cttgacaaat gttaaaaaag ccgttgcttt
      301 ggggataacc cggtaaggcc ggagttttat ctcgccacag agtaaatttt gctcatgatt
      361 gacagcggag tttacgctgt atcagaaata ttatggtga
//
LOCUS       DAZKFU020000804          397 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.814, whole genome shotgun sequence.
ACCESSION   DAZKFU020000804 DAZKFU020000000
VERSION     DAZKFU020000804.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.814"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>397)
                     /locus_tag="ACHOQQ_RS27120"
                     /old_locus_tag="pgaptmp_005424"
     CDS             complement(<1..>397)
                     /locus_tag="ACHOQQ_RS27120"
                     /old_locus_tag="pgaptmp_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HGU4473515.1"
                     /translation="GSYLFMQSFVQDCTIQGGYCPGSSILLSHFYNCTIAKLDAHGMD
                     FLANAFTKSSDNEARAQDQGTVFNYCNLNSINMKCSLGNASSDEYKFNPEEYFSDYIE
                     RRSRTLKLDDNFSNCVGNIEKAVKTSFSKQ"
BASE COUNT          134 a           92 c           55 g          116 t
ORIGIN      
        1 ctgcttgcta aatgaagttt taacagcctt ctcgatattc cctacgcagt tagaaaaatt
       61 atcatccaac ttaagagttc tcgatcttct ttcaatgtaa tcagaaaaat actcttctgg
      121 gttaaactta tattcatcgg aactcgcatt accaagacta cacttcatat taatactatt
      181 caaattacaa taattaaaca cagttccctg atcctgcgcc ctggcttcgt tatcactaga
      241 cttagtgaat gcgtttgcca aaaaatccat cccatgagca tcaagtttcg ctatcgtaca
      301 gttataaaaa tgactcagaa gtatactcga accaggacaa tatcccccct gaattgtgca
      361 atcctgaaca aaagactgca taaagagata agaacct
//
LOCUS       DAZKFU020000805          396 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.815, whole genome shotgun sequence.
ACCESSION   DAZKFU020000805 DAZKFU020000000
VERSION     DAZKFU020000805.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.815"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>396
                     /gene="espX1"
                     /locus_tag="ACHOQQ_RS27125"
                     /old_locus_tag="pgaptmp_005425"
     CDS             <1..>396
                     /gene="espX1"
                     /locus_tag="ACHOQQ_RS27125"
                     /old_locus_tag="pgaptmp_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HGU4473516.1"
                     /translation="FGCVLSNISCFESDLSNTIFSNGEINNLFIKKSNIFGTSFTNTM
                     IKNLRCEDIMPGRWTTQLVNKHLGFRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDN
                     VKLNNDVYKELKKFMHDYDKTSPEMRKYL"
BASE COUNT          147 a           74 c           62 g          113 t
ORIGIN      
        1 attttggttg tgtattatcc aatatatcat gttttgaaag cgacttatca aacaccatct
       61 tttcaaatgg agaaataaac aacctcttca ttaaaaaaag caacatattt ggaacatcgt
      121 tcactaatac catgataaaa aatttgcgct gtgaagatat tatgcctggc cgatggacca
      181 ctcaacttgt caataagcac ttaggtttca gatacacagg agtattcaaa accctggcaa
      241 gtattgatga caaaccgtcc cgtttcgaaa ttttgatacc attggtacaa actttagttc
      301 gcgacaatgt aaagctaaat aatgatgtgt ataaagaact gaaaaaattt atgcacgatt
      361 acgacaaaac atccccagaa atgagaaaat accttc
//
LOCUS       DAZKFU020000806          394 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.816, whole genome shotgun sequence.
ACCESSION   DAZKFU020000806 DAZKFU020000000
VERSION     DAZKFU020000806.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.816"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>394)
                     /locus_tag="ACHOQQ_RS27130"
                     /old_locus_tag="pgaptmp_005426"
     CDS             complement(<1..>394)
                     /locus_tag="ACHOQQ_RS27130"
                     /old_locus_tag="pgaptmp_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4473517.1"
                     /translation="KGALPPEQLNRCDMNTLQLEPTTFVDPDLRQFACDVLWSMKTTD
                     GRDGYIYTLTEHQSKADKYMALRMMRYVLAVIHRHLKAHACAPVVIPVLFYHGVPSPY
                     PYSMNWPDCLDDPAFGRELYGEGRPPRVI"
BASE COUNT           94 a          114 c           96 g           90 t
ORIGIN      
        1 caatcaccct gggtggcctg ccttctccat aaagctcccg accaaacgcc ggatcatcca
       61 gacagtcagg ccagttcatg ctgtatggat aaggactggg tacaccatgg taaaaaagca
      121 ccggtatcac aacgggagca cacgcatgtg ctttcaggtg gcgatgtatc accgccagaa
      181 catagcgcat cattcgcagg gccatatact tatccgcctt gctctggtgc tctgtcagcg
      241 tataaatgta tccatccctg ccatctgtgg ttttcatact ccacagaaca tcacaggcaa
      301 actggcgaag atccggatcc acaaacgtgg tcggctccag ttgcagggtg ttcatatcgc
      361 accggttcag ttgttccggg ggcaacgcgc cttt
//
LOCUS       DAZKFU020000807          394 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.817, whole genome shotgun sequence.
ACCESSION   DAZKFU020000807 DAZKFU020000000
VERSION     DAZKFU020000807.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.817"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>394)
                     /locus_tag="ACHOQQ_RS27135"
                     /old_locus_tag="pgaptmp_005427"
     CDS             complement(<1..>394)
                     /locus_tag="ACHOQQ_RS27135"
                     /old_locus_tag="pgaptmp_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGU4473518.1"
                     /translation="MAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFNYM
                     VKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRLEGIEKGRLEGIEKGR
                     QEEREKALQENYASARRMQQMGISSEKIK"
BASE COUNT           89 a          117 c           74 g          114 t
ORIGIN      
        1 tttgattttc tctgacgata tacccatctg ctgcatgcga cgggccgatg cgtaattctc
       61 ctgaagagcc ttttctctct cctcctggcg gcccttctcg ataccttcca ggcggccctt
      121 ctcaatacct tccaggcggc ccttctcgat accagccagt tccagtcgtt cagcgatagt
      181 cattagatca tcctcatgtc ttggcagacg ttctgccatc gcccgtataa attcaggtga
      241 agctgaatca cattctttca ccatatagtt aaacaatgtc accacctgag tgtgtgtttc
      301 ctgtatctga atcaactgtg acaaaaagtc aagatgaacc atcagatcca ctttcttctg
      361 ccggagcttc atcataagca tcagcgcagc catc
//
LOCUS       DAZKFU020000808          392 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.818, whole genome shotgun sequence.
ACCESSION   DAZKFU020000808 DAZKFU020000000
VERSION     DAZKFU020000808.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.818"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..180
                     /gene="ibsC"
                     /locus_tag="ACHOQQ_RS27140"
                     /old_locus_tag="pgaptmp_005428"
     CDS             121..180
                     /gene="ibsC"
                     /locus_tag="ACHOQQ_RS27140"
                     /old_locus_tag="pgaptmp_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGU4473519.1"
                     /translation="MMRLVIILIVLLLISFSAY"
BASE COUNT          108 a           84 c           93 g          107 t
ORIGIN      
        1 aaaaaattta aaggtagatc aatgcgttgt gcttatttaa agagagttta cgatggcagg
       61 gcagcatggg gctgtaacgg taaagccctc accgaagcga gggcttgaag gagaagggtt
      121 atgatgcgac ttgtcatcat actgattgta ctgttactca taagtttcag cgcttattaa
      181 cagtcagtca caggggagga gcaatcctcc cttaccctta ctcactaaat taggtcaaag
      241 aatcaacgat gtcaatcagg gcgatgcggg atgtatcgcc ctgaaccgat catttgatag
      301 acacttcatc gcccacgttt actgccgtaa ttaatccctt atccctgcaa tgtgtgtctg
      361 tcttacatga cgttttacga gtgcgctcga ag
//
LOCUS       DAZKFU020000809          392 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.819, whole genome shotgun sequence.
ACCESSION   DAZKFU020000809 DAZKFU020000000
VERSION     DAZKFU020000809.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.819"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..>392
                     /locus_tag="ACHOQQ_RS27145"
                     /old_locus_tag="pgaptmp_005429"
     CDS             51..>392
                     /locus_tag="ACHOQQ_RS27145"
                     /old_locus_tag="pgaptmp_005429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HGU4473520.1"
                     /translation="MIFAEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVAS
                     PEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDW
                     KKVGSLPEGLAY"
BASE COUNT          111 a           70 c          108 g          103 t
ORIGIN      
        1 agtggaagaa cctgggaacg actccatttc cgggaactgc tggttcatcg gtgatttttg
       61 cagaacagca aatttatata ctaggagggg agagaaaacc ggggcttcgt tctgttagat
      121 cctggacggg ggagctttcg catgatcgaa ttaaatggtc tgaacttcca ccagtagctt
      181 cacccgaggg ggtgtcagga gcatatgcct ctgttattga tggaaatatc tttctggccg
      241 gtggtgcata tttcccgggg gcggcagaaa agtatagcaa tggtgaatac tggtctcata
      301 aaggactgga taaagcctat tcaaaagaga tatatcagct aataaaaaat gactggaaaa
      361 aagttggttc tttgccggaa ggacttgctt at
//
LOCUS       DAZKFU020000810          392 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.820, whole genome shotgun sequence.
ACCESSION   DAZKFU020000810 DAZKFU020000000
VERSION     DAZKFU020000810.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.820"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..262
                     /locus_tag="ACHOQQ_RS27150"
                     /old_locus_tag="pgaptmp_005430"
     CDS             <1..262
                     /locus_tag="ACHOQQ_RS27150"
                     /old_locus_tag="pgaptmp_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473521.1"
                     /translation="SESFSSPSGLDNQGNIFVGHAKYVNEMDFTINSFSQKMYVAMVK
                     NVDNEVKLQCARYITTGSVDEQNSISDKIAFTFYGAATTADL"
BASE COUNT          119 a           58 c           81 g          134 t
ORIGIN      
        1 ttccgaaagt tttagttcac ccagtggttt ggacaaccag ggaaatattt ttgtgggtca
       61 tgctaaatat gttaatgaaa tggattttac tataaatagt tttagccaaa agatgtatgt
      121 agcgatggtg aaaaacgttg ataatgaagt taagcttcag tgcgctcgtt atattactac
      181 cggtagtgtc gacgaacaaa actccatcag cgataaaatc gcttttacat tttacggtgc
      241 cgcaacaact gctgatttat gatttttgcc tgataaatcg cgtttatcat taagttgatc
      301 gcgtattctt aaccgtctgt gtgtaaacag tctttttaaa aaaatgagcg atttttgata
      361 gtgaataagt agtgttagtg atgcaattgc at
//
LOCUS       DAZKFU020000811          391 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.821, whole genome shotgun sequence.
ACCESSION   DAZKFU020000811 DAZKFU020000000
VERSION     DAZKFU020000811.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.821"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            89..>391
                     /locus_tag="ACHOQQ_RS27155"
                     /old_locus_tag="pgaptmp_005431"
     CDS             89..>391
                     /locus_tag="ACHOQQ_RS27155"
                     /old_locus_tag="pgaptmp_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADH-specific enoyl-ACP
                     reductase/trans-2-enoyl-CoA reductase"
                     /protein_id="HGU4473522.1"
                     /translation="MIIKPVIKGVVSKSAHPEGCRHAIMEQIKYCQSHLQQINNGPKR
                     VLILGASSGFGLAARISLTFGGAKCDSIGVSFEKGPSEKSIGTAGWYNNIFFKEFAE"
BASE COUNT          120 a           67 c           83 g          121 t
ORIGIN      
        1 acaaatcaaa atgatgaggt tgtaatcgaa ggcattgcag tagtcatggc tccaaaatct
       61 taatttattt aactatcaag gaccttatat gattataaaa cctgtaatta aaggtgttgt
      121 ctcgaaaagt gctcatccag agggatgtcg tcatgcgata atggaacaga ttaagtattg
      181 ccagtcccat cttcagcaaa ttaataatgg cccgaaacga gttctgattc tcggtgcatc
      241 ttcaggattt ggtcttgctg caagaatttc tctcactttt ggcggcgcaa agtgcgattc
      301 tatcggagtt tcgtttgaaa aaggtccctc tgagaaatct ataggaactg caggatggta
      361 taataatata ttctttaagg aatttgctga a
//
LOCUS       DAZKFU020000812          389 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.822, whole genome shotgun sequence.
ACCESSION   DAZKFU020000812 DAZKFU020000000
VERSION     DAZKFU020000812.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.822"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            95..>389
                     /locus_tag="ACHOQQ_RS27160"
                     /old_locus_tag="pgaptmp_005432"
     CDS             95..>389
                     /locus_tag="ACHOQQ_RS27160"
                     /old_locus_tag="pgaptmp_005432"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4473523.1"
                     /translation="MDGIRIEWKTLGEVAVIGTGNHDTQDAIEHGKYIFYARGREPLK
                     LNVFDFDETAIITAGDGAGVGKVFHYAKGKYALHQRAYRIVPNAFMNPRFVYHY"
BASE COUNT          122 a           57 c           98 g          112 t
ORIGIN      
        1 aacatgcgta aaaaacaata caactactat cgagataagt tgttgaattt caaagatatt
       61 gcgggggggg ggtaattaca tccttccgga cgccatggat ggtattcgga ttgagtggaa
      121 gactttgggg gaagtggctg ttataggaac aggtaaccat gatacgcaag atgctataga
      181 acatggcaaa tatatttttt atgcacgtgg acgtgaacca ttaaagttaa atgtatttga
      241 ttttgatgaa acagcaatca ttacggctgg agatggtgcc ggggtgggga aggtatttca
      301 ttacgctaaa ggtaaatatg ctttgcatca gagagcatat cgtattgtcc caaatgcatt
      361 tatgaatccg cgatttgtat atcactata
//
LOCUS       DAZKFU020000813          389 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.823, whole genome shotgun sequence.
ACCESSION   DAZKFU020000813 DAZKFU020000000
VERSION     DAZKFU020000813.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.823"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            116..>389
                     /locus_tag="ACHOQQ_RS27165"
                     /old_locus_tag="pgaptmp_005433"
     CDS             116..>389
                     /locus_tag="ACHOQQ_RS27165"
                     /old_locus_tag="pgaptmp_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4473524.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRKLKPNTEPVNILLSNNFIRINKNITLSDLTHFLISNLHTQNVYS"
BASE COUNT          128 a           74 c           55 g          132 t
ORIGIN      
        1 acaatttaag gtataaaccc tattccctca atgttaagca aatataacaa ttactcttat
       61 tttaactttt acacgtaata tttaccctaa atgattttag agtacaggtt ggcatatgca
      121 agtcttttcc agtgatgtct acttcactgt aggcactaat gcattattag catcccaaaa
      181 agaatattac agcgatttag ttgcgttggt tgatcttggg cattcgttcg ttgttattga
      241 tgagcatcaa catcgaaagc tcaaaccaaa tacagaacct gtaaacatcc ttttaagcaa
      301 caatttcatc aggatcaata agaatattac gttatctgat ttaacgcatt tcctgatttc
      361 gaatcttcat acacaaaatg tgtattcca
//
LOCUS       DAZKFU020000814          389 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.824, whole genome shotgun sequence.
ACCESSION   DAZKFU020000814 DAZKFU020000000
VERSION     DAZKFU020000814.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.824"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..254
                     /locus_tag="ACHOQQ_RS27170"
                     /old_locus_tag="pgaptmp_005434"
     CDS             <1..254
                     /locus_tag="ACHOQQ_RS27170"
                     /old_locus_tag="pgaptmp_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4473525.1"
                     /translation="QLVAEELVEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSV
                     QRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVNGYSR"
     gene            238..>389
                     /locus_tag="ACHOQQ_RS27175"
                     /old_locus_tag="pgaptmp_005435"
     CDS             238..>389
                     /locus_tag="ACHOQQ_RS27175"
                     /old_locus_tag="pgaptmp_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGU4473526.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDA"
BASE COUNT           99 a           83 c          130 g           77 t
ORIGIN      
        1 agcaactggt ggcggaagag ctggtggaga agtgggaaaa aggaaagatg cgtctgctgt
       61 gggataacaa aaagcggcgt aacgaagcgc tggactgcct ggtgtatgcc tacgcggcat
      121 tacgtgtgtc cgtgcaacgc tggcagcttg atctggctgt actggcaaaa tcccgggaag
      181 aagagacgac ccggccaacc ctgaaagaac tggcagcgaa gctgtccgga ggagtgaatg
      241 gttacagtcg ctgaactgca ggcgctgcgt caggcgcgcc ttgatttatt aaccggtaaa
      301 cgggtggtgt ctgtccagaa agatggtcgc agaattgaat atacggcggc ttctctggat
      361 gagcttaacc gtgcgatcaa tgatgcgga
//
LOCUS       DAZKFU020000815          389 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.825, whole genome shotgun sequence.
ACCESSION   DAZKFU020000815 DAZKFU020000000
VERSION     DAZKFU020000815.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.825"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..389
                     /locus_tag="ACHOQQ_RS27180"
                     /old_locus_tag="pgaptmp_005436"
                     /pseudo
     CDS             1..389
                     /locus_tag="ACHOQQ_RS27180"
                     /old_locus_tag="pgaptmp_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          108 a           86 c          111 g           84 t
ORIGIN      
        1 caaagaagcg gtggagtatg gtcagtcgcg aaaatggata gcgcatgcgc ccttgttgcc
       61 aacggacaag gaataaccct ggtaagccgt attctgggcg tgtcgcgtgc gcaactggca
      121 caacggatga aaacatctgg taataagccg aataaacgtc gtcgcagatg tgatgaggct
      181 gcggatgcgg aagtgttagc acgtatcctt gatatcatca gcgatatgcc gacatatggc
      241 tatcgtcgtg tctgggccat tctcagacga cagtcccgta acaaaggagt gccgttcgtg
      301 aatgcaaagc gcgtttatcg gataatgagc gaaaataacc tgttgttgtt acatgacaaa
      361 ccttcacggc cgcagcggga acataaagg
//
LOCUS       DAZKFU020000816          388 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.826, whole genome shotgun sequence.
ACCESSION   DAZKFU020000816 DAZKFU020000000
VERSION     DAZKFU020000816.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.826"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..>388)
                     /locus_tag="ACHOQQ_RS27185"
                     /old_locus_tag="pgaptmp_005437"
     CDS             complement(87..>388)
                     /locus_tag="ACHOQQ_RS27185"
                     /old_locus_tag="pgaptmp_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473527.1"
                     /translation="NELKGSWISGPTLTSGSSWNNACEENGVTFSLSEDNFSIDPVLG
                     YTGHAAPWLAITLADAAFEQRGAQVIAAQPTADKDDVWVAVITKEEVRKESPGNG"
BASE COUNT           97 a           98 c          101 g           92 t
ORIGIN      
        1 gaaaggcata aaaaagaaat gccacgatga ccgtcagaaa aaaggcgaca gtcacaacag
       61 agccgatgag tgttttaact tttaccttag ccattcccgg gtgattcctt gcgaacttct
      121 tctttagtga tgacggcaac ccagacgtcg tctttgtcag ctgtgggctg ggcggcgata
      181 acctgcgcac ctcgttgttc aaaggcagca tcagcaagcg tgatggcaag ccatggagca
      241 gcatggccgg tatagcccag cacgggatcg atactgaaat tgtcttctga aaggctgaat
      301 gtcactccgt tctcttcgca ggcattattc cagctgctgc ccgacgtcaa tgtgggaccg
      361 gagatccagc ttcctttaag ctcattgg
//
LOCUS       DAZKFU020000817          387 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.827, whole genome shotgun sequence.
ACCESSION   DAZKFU020000817 DAZKFU020000000
VERSION     DAZKFU020000817.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.827"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     repeat_region   22..355
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=22..50
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT           81 a          108 c          126 g           72 t
ORIGIN      
        1 taataatagg ttatgtttag agagttcccc gcgccagcgg ggataaaccg ggcgcttcgg
       61 tgtcgatctg ggcgttgatg gcgagttccc cgcgccagcg gggataaacc gatgtcggct
      121 gcaatctgcc gcgccgggcg tgtgagttcc ccgcgccagc ggggataaac cggctggata
      181 cagtctggca gggttctgtg atgagagttc cccgcgccag cggggataaa ccgatgatgc
      241 agccgtctca ggacgacctt cgcacgagtt ccccgcgcca gcggggataa accgaacggc
      301 tacggcactg aggtgacaaa ccagatgagt tccccgcgcc agcggggata aaccggtttc
      361 tggcatcgga aaacattgca tatcgtc
//
LOCUS       DAZKFU020000818          382 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.829, whole genome shotgun sequence.
ACCESSION   DAZKFU020000818 DAZKFU020000000
VERSION     DAZKFU020000818.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.829"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>382
                     /locus_tag="ACHOQQ_RS27190"
                     /old_locus_tag="pgaptmp_005438"
     CDS             <1..>382
                     /locus_tag="ACHOQQ_RS27190"
                     /old_locus_tag="pgaptmp_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HGU4473528.1"
                     /translation="QELLNHLDQQQANDVLAVLQRHNINAEKKDQGKTGFSIFVEPTD
                     FASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKARLYSAIEQRLEQSLKIMDG
                     IVSSRVHVSYDVDTGDSGKTALPIH"
BASE COUNT          118 a           72 c           79 g          113 t
ORIGIN      
        1 caggaattat tgaaccatct tgatcaacag caagctaatg atgtccttgc tgttttacaa
       61 agacataata ttaatgctga aaagaaggat caaggcaaaa caggattttc aatatttgtc
      121 gaaccaacgg attttgcttc tgcggtggat tggctaaaaa tctacaatct cccgggtaag
      181 cctgatattc aaatatcgca gatgttccct gcagatgcac tcgtttcatc tccacgagcg
      241 gaaaaagcaa ggctatattc tgctattgag cagcggttag agcaatctct caaaattatg
      301 gatggcattg tttcttcgag agtacatgtt agttacgatg ttgatactgg cgatagtggt
      361 aaaactgctt taccaattca ta
//
LOCUS       DAZKFU020000819          380 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.830, whole genome shotgun sequence.
ACCESSION   DAZKFU020000819 DAZKFU020000000
VERSION     DAZKFU020000819.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.830"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>380
                     /locus_tag="ACHOQQ_RS27195"
                     /old_locus_tag="pgaptmp_005439"
     CDS             <1..>380
                     /locus_tag="ACHOQQ_RS27195"
                     /old_locus_tag="pgaptmp_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4473529.1"
                     /translation="SQYLTSVILNNLPERPFNIRMVRETADSTTDQLQNRTLWSSYTE
                     IIDVKQCYPNTAIVGLQVDAEQFGGQQLTVNYHIRGRIIQVPSNYDPEKRTYSGIWDG
                     SLKPAYSNNPAWCLWDMLTHPRYG"
BASE COUNT           91 a          109 c          111 g           69 t
ORIGIN      
        1 cctcgcagta cctgacgtcg gtgattctga ataatctccc tgagcgcccc tttaacatcc
       61 ggatggtcag ggagacggcg gacagcacca cggaccagct gcagaacaga acgctgtggt
      121 cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcc attgtggggc
      181 tgcaggtgga tgcggagcag tttggtggcc agcagctgac ggtgaactac catatccgtg
      241 gtcgcatcat tcaggtgccg tcaaactatg acccggaaaa acgcacctac agcggtatct
      301 gggacgggag tctgaaaccg gcatacagca ataacccggc ctggtgcctg tgggacatgc
      361 tgacccaccc gcgctacggg
//
LOCUS       DAZKFU020000820          380 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.831, whole genome shotgun sequence.
ACCESSION   DAZKFU020000820 DAZKFU020000000
VERSION     DAZKFU020000820.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.831"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..237
                     /locus_tag="ACHOQQ_RS27200"
                     /old_locus_tag="pgaptmp_005440"
     CDS             <1..237
                     /locus_tag="ACHOQQ_RS27200"
                     /old_locus_tag="pgaptmp_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU4473530.1"
                     /translation="RTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPYVTIDVSSKS
                     HPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
BASE COUNT           95 a           88 c           97 g          100 t
ORIGIN      
        1 cgcacagtag tgttccatga cacgagtgtg gatgagtact ttaaaatagg ttctaccatc
       61 aaaacagatc gcgagattga actggatggt gtgacgtatc cgtatgtgac gattgacgtt
      121 tcctccaaat cgcatccgtt ctataccggt aaactcagaa cggtggcatc agaaggcaat
      181 gttgcccgct ttactcagcg ttttggtcgt ttcgttggga ctcagaaggg agaatgatga
      241 gagtgagagt cttaacaggc tccatgcgtg gtggatttgc tcctatattt ccagacatct
      301 ggtatcactt aacccattac tgcccgccgc cgcagatatt cccgtggaga gcgataaccc
      361 agcgcactat gtggatgcca
//
LOCUS       DAZKFU020000821          378 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.832, whole genome shotgun sequence.
ACCESSION   DAZKFU020000821 DAZKFU020000000
VERSION     DAZKFU020000821.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.832"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>378
                     /locus_tag="ACHOQQ_RS27205"
                     /old_locus_tag="pgaptmp_005441"
     CDS             <1..>378
                     /locus_tag="ACHOQQ_RS27205"
                     /old_locus_tag="pgaptmp_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4473531.1"
                     /translation="EGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYG
                     LNVVSGDITDITTPDGRETKFYYNDGNLVTAVVYPDGLESRRAYDERDRLVTETSRSG
                     ETVRYRYDDAHSELPATTTDATG"
BASE COUNT           94 a           93 c          146 g           45 t
ORIGIN      
        1 cagaaggcgg ggccgggctg aagcgggtgg tgaaaaaaga actggcggac ggcagcgtca
       61 cgcacagcgg gtatgacgcg gcaggaaggc tcacggcgca gacggacgcg gcgggacggc
      121 ggacagagta cggtctgaat gtggtgtcag gcgacatcac ggacatcacc acaccggacg
      181 ggcgggagac gaaattttac tataacgacg ggaacctggt gacggcggtg gtgtacccgg
      241 acgggctgga gagccgccgg gcgtatgatg agcgggacag gctggtaacg gagacatcgc
      301 gcagcgggga gacagtacgc taccgctatg atgatgcgca cagtgaatta ccggccacaa
      361 caacagatgc gacgggca
//
LOCUS       DAZKFU020000822          375 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.833, whole genome shotgun sequence.
ACCESSION   DAZKFU020000822 DAZKFU020000000
VERSION     DAZKFU020000822.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.833"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>375
                     /locus_tag="ACHOQQ_RS27210"
                     /old_locus_tag="pgaptmp_005442"
     CDS             <1..>375
                     /locus_tag="ACHOQQ_RS27210"
                     /old_locus_tag="pgaptmp_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4473532.1"
                     /translation="YGKPSLIMVDYLQLIRAPELENRTQEIAEISRSLKALGKELGCP
                     VLALSQLNRQVEQRADKRPNNGDLRDSGALEQDADLIMFIYRDEVYNPDSLAKGEAEI
                     IVSKQRQGPTGTVRVRFDGRYT"
BASE COUNT           84 a           99 c          115 g           77 t
ORIGIN      
        1 tgtacggtaa accctccctg attatggtgg attatctgca actgattcgt gcgccggagc
       61 tggaaaaccg cacacaggaa attgctgaaa tttcccgttc cctgaaagcg ctggggaagg
      121 agctgggctg cccggtgctg gccctctccc agcttaaccg gcaggtggag cagcgtgctg
      181 ataaacgccc caataacggt gacctgcgtg attccggggc gctggagcag gatgctgacc
      241 tgattatgtt tatttaccgc gacgaggtct ataaccccga ctcactggca aagggggagg
      301 cagaaatcat tgtcagcaag cagcgtcagg ggcccacggg tacggtacgg gtcaggttcg
      361 acggtcgtta cacac
//
LOCUS       DAZKFU020000823          373 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.834, whole genome shotgun sequence.
ACCESSION   DAZKFU020000823 DAZKFU020000000
VERSION     DAZKFU020000823.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.834"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(69..>373)
                     /locus_tag="ACHOQQ_RS27215"
                     /old_locus_tag="pgaptmp_005443"
     CDS             complement(69..>373)
                     /locus_tag="ACHOQQ_RS27215"
                     /old_locus_tag="pgaptmp_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473533.1"
                     /translation="ASKGLNFSHSLAAINDMIWSTSVQYGPTTGLISRALKGKNLETL
                     TDADVITIVQDYKINHVEDLFPSSPTWWDDLKTRALTEKKDLLELNELNLELKNED"
BASE COUNT          119 a           71 c           63 g          120 t
ORIGIN      
        1 aggaattgca aacgtgtggt tctttcgcaa aagagataat cggcaagaaa ataagcaatg
       61 caaataattt aatcttcatt ctttaactcc agattcaatt catttaattc caataaatct
      121 ttcttttctg ttagcgctcg tgtctttaaa tcatcccacc aagtcgggga tgatggaaaa
      181 agatcttcaa catgatttat tttgtagtcc tgaacaatag ttatcacatc tgcatctgtt
      241 aatgtttcta aattctttcc tttcaacgct ctcgaaatta aaccagtcgt agggccatat
      301 tgaacggaag tcgaccaaat catatcattg attgcggcaa gagaatgaga gaaatttagc
      361 cctttggatg cca
//
LOCUS       DAZKFU020000824          371 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.835, whole genome shotgun sequence.
ACCESSION   DAZKFU020000824 DAZKFU020000000
VERSION     DAZKFU020000824.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.835"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..285
                     /locus_tag="ACHOQQ_RS27220"
                     /old_locus_tag="pgaptmp_005444"
     CDS             <1..285
                     /locus_tag="ACHOQQ_RS27220"
                     /old_locus_tag="pgaptmp_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4473534.1"
                     /translation="RSNTMAPTPVYADYLNNTTDFRPDPEDIFKCGQTLECGDPRKNI
                     RQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPR"
BASE COUNT          116 a           65 c           77 g          113 t
ORIGIN      
        1 agaagcaata ctatggcacc cacacctgta tatgctgatt atttaaataa cactactgat
       61 ttcagaccag atcctgaaga tatcttcaaa tgtggtcaga cgctcgaatg tggcgacccc
      121 cgcaaaaaca ttagacaaaa atatggccat catgggttag gggtaaatca gaatcaacaa
      181 tttgataatg gtgttatggg gctaaattcc aggatggcag tgtttgatga aaataaaaaa
      241 ataataggtt cttcctcagg gcttagttgt tttcaacctc gttaaaactc catgagtgct
      301 gtggtatgtt ttttaatcct gccagtattc cataggtttt cttaatcgat tgtagaggta
      361 tgtactgatg a
//
LOCUS       DAZKFU020000825          371 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.836, whole genome shotgun sequence.
ACCESSION   DAZKFU020000825 DAZKFU020000000
VERSION     DAZKFU020000825.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.836"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>371)
                     /locus_tag="ACHOQQ_RS27225"
                     /old_locus_tag="pgaptmp_005445"
     CDS             complement(<1..>371)
                     /locus_tag="ACHOQQ_RS27225"
                     /old_locus_tag="pgaptmp_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HGU4473535.1"
                     /translation="LVATLFLLGWLTEKAFDFAGKSGLYFQWGQKKAESITPVTNTIP
                     GEEDDDEPVFSEDAVSEHLRFRYGRRWQRKVRLLLVMGDPDVVRKAAPGLCHDLWQEG
                     DGYVLIYGGDAQSLPDEIFLS"
BASE COUNT           86 a          119 c           92 g           74 t
ORIGIN      
        1 tttgaaagga agatctcgtc cgggagtgac tgcgcgtctc cgccataaat cagcacgtag
       61 ccgtcgcctt cctgccagag gtcatggcaa aggcccgggg ctgctttccg gaccacatcc
      121 ggatccccca tcaccagcag gagtcgcacc ttacgctgcc agcgacgccc atagcgaaag
      181 cgcaaatgtt ctgaaacagc atcttcgcta aaaaccggct catcgtcgtc ttcttcaccg
      241 ggaatggtgt tcgtcaccgg agtgatactc tcagcctttt tctgccccca ctggaaatac
      301 agcccggatt taccggcgaa gtcgaaagcc ttctccgtca gccagccgag taaaaacagc
      361 gtggcaacca g
//
LOCUS       DAZKFU020000826          370 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.837, whole genome shotgun sequence.
ACCESSION   DAZKFU020000826 DAZKFU020000000
VERSION     DAZKFU020000826.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.837"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>370)
                     /locus_tag="ACHOQQ_RS27230"
                     /old_locus_tag="pgaptmp_005446"
     CDS             complement(<1..>370)
                     /locus_tag="ACHOQQ_RS27230"
                     /old_locus_tag="pgaptmp_005446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473536.1"
                     /translation="AGCLPAYVAPGSAQFINAIPIPVFNTTIYSGLVIGIVGTAVWLL
                     FAVWYMSAKIKNAKIKGEGYGTEVSNDCNLETGKVTLAFIPILIVIIGNAFLTYFFQI
                     DEVVDYYRDFGGVQGTWAILI"
BASE COUNT          130 a           79 c           49 g          112 t
ORIGIN      
        1 aaattaaaat tgcccatgtt ccctgaacac ctccaaaatc tctgtaataa tcaaccactt
       61 cgtcaatctg gaaaaaatat gttaaaaatg catttcctat aataactatc agtataggta
      121 taaatgccaa agtaactttt ccagtctcaa gattacagtc attacttact tctgtaccat
      181 atccttctcc ttttatctta gcatttttaa tttttgcaga catataccat acagcgaaca
      241 ataaccatac agcagtgcca acgataccaa ttactaaacc tgaatatatg gttgtattga
      301 acacaggaat tggtattgca tttataaact gggcagaacc aggagctaca tatgctggta
      361 agcaaccagc
//
LOCUS       DAZKFU020000827          366 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.838, whole genome shotgun sequence.
ACCESSION   DAZKFU020000827 DAZKFU020000000
VERSION     DAZKFU020000827.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.838"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            174..>366
                     /locus_tag="ACHOQQ_RS27235"
                     /old_locus_tag="pgaptmp_005447"
     CDS             174..>366
                     /locus_tag="ACHOQQ_RS27235"
                     /old_locus_tag="pgaptmp_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU4473537.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVD"
BASE COUNT          118 a           64 c           89 g           95 t
ORIGIN      
        1 ggcgataact aaaccatttt tgagtaacaa atgaaacaac tgcaacgctc ttattatttc
       61 tggaaaacgt ctcgcaaaaa caaaacaaca tgcaaaaatc cgctttcgtt aattaatttt
      121 ccggcgtaat tttcgcacat acattaatga gtaatatatg taagagggtg gcaatggcga
      181 tgaatacggt ttttcttcat ttatcagaag aggcaattaa acggctgaac aagcttcggg
      241 gctggcgtaa ggtttcacga tctgcaattt tacgcgaagc ggtagagcaa tatctggaac
      301 gtcagcaatt cccggtgcgt aaagcaaagg gaggcaggca aaagggggag gtagttggtg
      361 ttgatg
//
LOCUS       DAZKFU020000828          365 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.839, whole genome shotgun sequence.
ACCESSION   DAZKFU020000828 DAZKFU020000000
VERSION     DAZKFU020000828.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.839"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>365)
                     /locus_tag="ACHOQQ_RS27240"
                     /old_locus_tag="pgaptmp_005448"
     CDS             complement(<1..>365)
                     /locus_tag="ACHOQQ_RS27240"
                     /old_locus_tag="pgaptmp_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4473538.1"
                     /translation="LQPYLVINKGAVTPCTFLGDQTGDRFSNICGDQFIIDLLKCIMS
                     INDNVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDSKYKIMANHHG
                     ITFDDQRKRYIIGPITVSI"
BASE COUNT          111 a           72 c           63 g          119 t
ORIGIN      
        1 aatagagacc gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
       61 atgatgattt gccattattt tgtatttgga gtcatagaaa catacaggat aatcttttat
      121 cccagcgtag gtatcctcat caagtggttt catacgcgtg aagttctgca tatagttacc
      181 attgcaattc gtttcatgat ttccagctaa tacatgaacg ttatcattga tgctcattat
      241 gcattttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
      301 ttggtcaccc aaaaacgtac atggcgtcac tgcgccttta ttaataacta aataaggctg
      361 aagac
//
LOCUS       DAZKFU020000829          365 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.840, whole genome shotgun sequence.
ACCESSION   DAZKFU020000829 DAZKFU020000000
VERSION     DAZKFU020000829.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.840"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>365)
                     /locus_tag="ACHOQQ_RS27245"
                     /old_locus_tag="pgaptmp_005449"
     CDS             complement(<1..>365)
                     /locus_tag="ACHOQQ_RS27245"
                     /old_locus_tag="pgaptmp_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4473539.1"
                     /translation="TGVACSVCPGGMTSGNPVNPLLGAKVLPGETDLALPGPLPFILS
                     RTYSSYRTKTPAPVGIFGPGWKAPSDIRLQLRDDALVLNDNGGRSIHFEPLLPGEAVY
                     SRSESMWLVRGGKAAQPDG"
BASE COUNT           65 a          127 c          112 g           61 t
ORIGIN      
        1 tggccgtccg gctgtgctgc cttaccaccg cgcaccagcc acattgactc gctgcggctg
       61 tacaccgcct cacccggcag cagcggctca aagtgaatgc tccgtccgcc gttgtcgttg
      121 agtaccagtg catcatcgcg tagctgtaag cggatatcag aaggcgcttt ccagccgggg
      181 ccaaaaatcc ccaccggtgc aggcgtcttc gtccggtagc tgctgtaggt gcgggagaga
      241 atgaacggca gcgggccggg cagcgcaagg tccgtctcgc cgggcagcac cttcgccccc
      301 agcagcggat ttaccgggtt gcccgaggtc atcccgcccg gacacaccga gcacgccacg
      361 ccagt
//
LOCUS       DAZKFU020000830          362 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.841, whole genome shotgun sequence.
ACCESSION   DAZKFU020000830 DAZKFU020000000
VERSION     DAZKFU020000830.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.841"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>362)
                     /locus_tag="ACHOQQ_RS27250"
                     /old_locus_tag="pgaptmp_005450"
     CDS             complement(<1..>362)
                     /locus_tag="ACHOQQ_RS27250"
                     /old_locus_tag="pgaptmp_005450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HGU4473540.1"
                     /translation="GVIKVLPVFTLIQTTIFSIPGVALFPAIIGTAILSGIVGSTSGG
                     VGLVMVTFGQDLLELSQAQNISPGLMHRIIVFSASTLDTLPHSGFIITLLGVCGLSHK
                     QSYKELFIVTCVFPAFAV"
BASE COUNT          136 a           75 c           63 g           88 t
ORIGIN      
        1 aaccgcaaat gcgggaaaca cacaagtaac aataaaaagc tctttataac tttgcttatg
       61 agataagcca caaactccta gcaatgtgat aataaagcca gaatgaggaa gcgtatccag
      121 cgttgatgca ctaaaaacaa taatacgatg cattaaacct ggcgaaatat tctgagcttg
      181 agatagttct aataagtctt gaccaaacgt taccataact aatcccaccc ctcctgatgt
      241 tgagcccact attcccgaaa gaattgctgt acctataatt gcaggaaata atgctactcc
      301 aggaatagaa aaaattgttg tttgaataag ggtaaacaca ggaaggactt taattacccc
      361 ac
//
LOCUS       DAZKFU020000831          361 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.842, whole genome shotgun sequence.
ACCESSION   DAZKFU020000831 DAZKFU020000000
VERSION     DAZKFU020000831.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.842"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..315
                     /locus_tag="ACHOQQ_RS27255"
                     /old_locus_tag="pgaptmp_005451"
     CDS             <1..315
                     /locus_tag="ACHOQQ_RS27255"
                     /old_locus_tag="pgaptmp_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HGU4473541.1"
                     /translation="TGALTGDTGGRLALFVKAKECGTCDARLASVLATGRQVDIYLVD
                     SQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRFGEGKMPVVLQQGADGWRVA
                     AF"
BASE COUNT           68 a           84 c          129 g           80 t
ORIGIN      
        1 acaggagcac ttaccggaga tacgggggga cggctggcgc tgtttgtgaa ggcgaaggaa
       61 tgtggcacct gtgatgcccg tcttgcctcc gtactggcca cgggacggca ggttgatatc
      121 taccttgtcg acagtcaggg aaaggatgag attctgcgtc agtgggcgca tgcgcacagc
      181 atacctgtgg agaaagttcg cagccgtcat atcaccctga atcatgatgc cggacgctgg
      241 ctgcgttttg gtgaaggaaa aatgccggtg gtactgcagc agggggctga cggatggcgc
      301 gtggcggcat tctgacggtt ctgctgatgg tggcctgcag tggtgtctgt cgtgctgccg
      361 t
//
LOCUS       DAZKFU020000832          361 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.843, whole genome shotgun sequence.
ACCESSION   DAZKFU020000832 DAZKFU020000000
VERSION     DAZKFU020000832.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.843"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS27260"
                     /old_locus_tag="pgaptmp_005452"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS27260"
                     /old_locus_tag="pgaptmp_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4473542.1"
                     /translation="MKIYRPLWSEGVLLSPQQFQQQTEWESFRSAGISALASPFPWGV
                     EKVELNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADHDAVTVV
                     IALPHMQPGVINVEQ"
BASE COUNT           93 a          105 c           89 g           74 t
ORIGIN      
        1 tcctgctcaa cattaatcac gccaggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgtggt cagccagctg accggcatct aactctcgcg gtgccggagg caaatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 agcccggatg acagaagact gtcattaagc tcaacctttt ccactcccca gggaaacggg
      241 gatgccagcg cagaaatacc ggcactgcga aaagactccc attcagtctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggtaaatctt catttcccga
      361 c
//
LOCUS       DAZKFU020000833          361 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.844, whole genome shotgun sequence.
ACCESSION   DAZKFU020000833 DAZKFU020000000
VERSION     DAZKFU020000833.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.844"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS27265"
                     /old_locus_tag="pgaptmp_005453"
     CDS             complement(<1..353)
                     /gene="tssK"
                     /locus_tag="ACHOQQ_RS27265"
                     /old_locus_tag="pgaptmp_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4473543.1"
                     /translation="MKIFRPLWSEGVLLSPQQFQQQAEWESFRSAGISALASPFPWGV
                     EQVEFNDSLLSSGLIQITQLRLWLEDGTLIDAQRSDLPPAPRELDAGQLADRDAVTVV
                     IALPHMQPGVANVEQ"
BASE COUNT           92 a          108 c           93 g           68 t
ORIGIN      
        1 tcctgctcaa cgttggcaac gcctggctgc atgtggggaa gtgcaataac gacagttacc
       61 gcatcgcggt cagccagctg accggcatct aactcacgtg gcgccggagg cagatcgctc
      121 ctttgagcat caataagtgt tccgtcttcc agccagaggc gtaactgcgt aatctggatc
      181 aacccggacg acagaagact gtcattaaac tcaacctgtt ccactcccca gggaaacggg
      241 gatgccagcg cagagatacc ggcactgcga aaagactccc attcagcctg ctgctgaaac
      301 tgctgagggg agagcaaaac accttcgctc cacagcggac ggaaaatctt catttcccga
      361 c
//
LOCUS       DAZKFU020000834          356 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.845, whole genome shotgun sequence.
ACCESSION   DAZKFU020000834 DAZKFU020000000
VERSION     DAZKFU020000834.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.845"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..314
                     /locus_tag="ACHOQQ_RS27270"
                     /old_locus_tag="pgaptmp_005454"
     CDS             <1..314
                     /locus_tag="ACHOQQ_RS27270"
                     /old_locus_tag="pgaptmp_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4473544.1"
                     /translation="CMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPFHRCMRG
                     TSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARAKYGLPE
                     I"
BASE COUNT           92 a           93 c           91 g           80 t
ORIGIN      
        1 catgcatgac gtgtggtgcc tgttgtgcat ttttccgcgt ctctttttac tgggcagaag
       61 ctgacgatgc tggcggaaag gttccggtca gtctcactga gcaaatatcc ccctttcatc
      121 gctgtatgcg cggcaccagt cagaaaaatc cccgatgtgt cgctctggcg ggaactccgg
      181 gtaaaaacgc atattgctct gtttacaaaa accgtccgtc cacatgccgg gaattcgcaa
      241 tgtccggaga aaatggcatc gcgaatgaag catgcaaccg cgcccgggca aaatacggac
      301 ttccggaaat ctgaaaaatt actcttatca gagaagagca caggaacacg atcggc
//
LOCUS       DAZKFU020000835          356 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.846, whole genome shotgun sequence.
ACCESSION   DAZKFU020000835 DAZKFU020000000
VERSION     DAZKFU020000835.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.846"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>356
                     /locus_tag="ACHOQQ_RS27275"
                     /old_locus_tag="pgaptmp_005455"
     CDS             <1..>356
                     /locus_tag="ACHOQQ_RS27275"
                     /old_locus_tag="pgaptmp_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HGU4473545.1"
                     /translation="RDDENKLVAKGDYGNPDAMVHDIRSSVLDEWHKDAPDLVVLMGR
                     DLFNTLRLPMINAISTTNPNTELVAGQLIVASRTVGGLQTYLAPYFPKDAMLITSLKN
                     LSIYFQKGSLRRFIRE"
BASE COUNT           82 a          101 c           94 g           79 t
ORIGIN      
        1 cgcgatgatg aaaacaaact ggtggcaaaa ggtgactatg gtaacccgga tgccatggtg
       61 cacgacatcc gctcatccgt gctggatgaa tggcataagg acgcaccgga tctggtcgta
      121 ctgatggggc gggatctgtt caatacactg cgtcttccca tgattaacgc catcagcacc
      181 accaacccga acactgagct tgttgccggg cagctgattg tggcgtcccg taccgtcggt
      241 ggtcttcaga cgtatcttgc gccgtacttc ccgaaagacg ccatgctcat cacgtcgctg
      301 aaaaacctgt cgatctactt ccagaaaggc tcactgcgcc gtttcattcg cgagga
//
LOCUS       DAZKFU020000836          356 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.847, whole genome shotgun sequence.
ACCESSION   DAZKFU020000836 DAZKFU020000000
VERSION     DAZKFU020000836.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.847"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..>356
                     /locus_tag="ACHOQQ_RS27280"
                     /old_locus_tag="pgaptmp_005456"
     CDS             111..>356
                     /locus_tag="ACHOQQ_RS27280"
                     /old_locus_tag="pgaptmp_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HGU4473546.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRS"
BASE COUNT           77 a           97 c          113 g           69 t
ORIGIN      
        1 tggcgggtgc tgaagaaaga attctcagtc ctgaacacag acctgatgcg cccgggttac
       61 ggcgaagggc ggtagcgtgc ctgacggtct cctgacagtg agtgaataac atggaaaatc
      121 cggcagccct gttacgtcgt cttaatcatt actgtgcccg tgcactggaa ggcgcagcct
      181 ccctttgcca gacccgggcc catgcagaaa tcaccccgga gcactggtta ctgaaactgc
      241 tggagcaggg ggaaggagac cttaccgtgc tgggccggcg ttatgactgg gatatggacg
      301 cgatatggca gtcgctgctc ggctggctgg ataaccagcc ccgtagcgta cgcagc
//
LOCUS       DAZKFU020000837          355 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.848, whole genome shotgun sequence.
ACCESSION   DAZKFU020000837 DAZKFU020000000
VERSION     DAZKFU020000837.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.848"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..346)
                     /gene="dinI"
                     /locus_tag="ACHOQQ_RS27285"
                     /old_locus_tag="pgaptmp_005457"
     CDS             complement(101..346)
                     /gene="dinI"
                     /locus_tag="ACHOQQ_RS27285"
                     /old_locus_tag="pgaptmp_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HGU4473547.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT          102 a           82 c           78 g           93 t
ORIGIN      
        1 tgtgcaatta ttttgaggaa gtgtagaaat tttgtactca aaattcgtaa gtaaaataaa
       61 aaagccgggg cgacccggca aaaacaaaaa tcactgcata ttattcgctg acaaaccagt
      121 catcggcgct ttcccacgtt tcctggagaa tttcgctaat gcgctgttta tcttcttttg
      181 tcgcgccaat aaccgataaa ttattcgctg cggcgtaacg taccgatacg tggccttcat
      241 tatcaggaaa cgcatactga atacggcggg aaagttcgcc agccagggcg tcaatagccc
      301 cagctggcaa tggagaagtt ttcgctatgg tgacttcaat tcgcataata gcccc
//
LOCUS       DAZKFU020000838          355 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.849, whole genome shotgun sequence.
ACCESSION   DAZKFU020000838 DAZKFU020000000
VERSION     DAZKFU020000838.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.849"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(107..>355)
                     /locus_tag="ACHOQQ_RS27290"
                     /old_locus_tag="pgaptmp_005458"
     CDS             complement(107..>355)
                     /locus_tag="ACHOQQ_RS27290"
                     /old_locus_tag="pgaptmp_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tox-REase-5 domain-containing protein"
                     /protein_id="HGU4473548.1"
                     /translation="KDKLCQFWEAKARYDQFFRADGSKKPWWTGNLSAINQASRHQAV
                     ATVNQPLKVVWIFMQPVSCEYFRKMFKDYKDIITRWQP"
BASE COUNT           98 a           79 c           54 g          124 t
ORIGIN      
        1 tttttaacaa ctacggtact tctgtatagt tcattcaaac aatcgtctat cgttatgtct
       61 ctttctttca aagaatgtat atttattgac gtgttaaata attcaatcac ggttgccacc
      121 tcgtaataat atccttgtaa tctttaaaca ttttcctgaa atactcacaa cttacaggtt
      181 gcataaatat ccagacaact ttcaatggct gattgactgt tgcaaccgcc tgatgtcggg
      241 atgcttgatt aattgcgctt agattacctg tccaccaggg tttctttgaa ccatcagccc
      301 tgaaaaattg gtcataccgc gctttagctt cccagaactg acacagctta tcttt
//
LOCUS       DAZKFU020000839          354 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.850, whole genome shotgun sequence.
ACCESSION   DAZKFU020000839 DAZKFU020000000
VERSION     DAZKFU020000839.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.850"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..283)
                     /locus_tag="ACHOQQ_RS27295"
                     /old_locus_tag="pgaptmp_005459"
     CDS             complement(77..283)
                     /locus_tag="ACHOQQ_RS27295"
                     /old_locus_tag="pgaptmp_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGU4473549.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT           98 a           91 c           75 g           90 t
ORIGIN      
        1 cctctgaaca tcttcctgat acaaaaaacc gccagcgttt ccgctggcgg ttttcaatgt
       61 attcagaact cagcaatcaa taaaaatcac cggttgcctt ttcggcctga tccatccaca
      121 ccggtttatc gctggttttc gaccagacgc gatgcaggta gctgtaaaag cgggcgcggt
      181 ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca taagtacggc
      241 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      301 gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaat
//
LOCUS       DAZKFU020000840          352 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.851, whole genome shotgun sequence.
ACCESSION   DAZKFU020000840 DAZKFU020000000
VERSION     DAZKFU020000840.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.851"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>352
                     /locus_tag="ACHOQQ_RS27300"
                     /old_locus_tag="pgaptmp_005460"
     CDS             <1..>352
                     /locus_tag="ACHOQQ_RS27300"
                     /old_locus_tag="pgaptmp_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HGU4473550.1"
                     /translation="QPVDGIIQVMSISALPTDGERDALLRYRQKADHQLGWQAPVWLW
                     LTDNAIGARQDAKTAATGVIFGPEGTVKGADEAFSTLALHLQKAGMAQILNEPADFGL
                     LQLSLRLRQELKASL"
BASE COUNT           81 a           99 c          108 g           64 t
ORIGIN      
        1 caaccggttg atggcatcat tcaggtgatg agcatttcag ccctcccgac ggatggcgaa
       61 cgggatgccc tcctgcgtta ccgccagaaa gccgaccatc agctgggctg gcaggcaccg
      121 gtctggctgt ggctgactga caacgctatc ggtgcccggc aggacgcgaa aacggccgca
      181 acaggggtca tcttcggacc ggaaggaacc gtgaaaggtg cggacgaagc gttttccaca
      241 ctggcgttac acctgcaaaa agcaggaatg gcgcagatac tgaatgaacc tgctgatttc
      301 ggtctccttc agttgtcact cagactgcgg caggagctga aagccagcct gg
//
LOCUS       DAZKFU020000841          352 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.852, whole genome shotgun sequence.
ACCESSION   DAZKFU020000841 DAZKFU020000000
VERSION     DAZKFU020000841.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.852"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>352
                     /locus_tag="ACHOQQ_RS27305"
                     /old_locus_tag="pgaptmp_005461"
     CDS             <1..>352
                     /locus_tag="ACHOQQ_RS27305"
                     /old_locus_tag="pgaptmp_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HGU4473551.1"
                     /translation="QPVDGIIQVMSISALLTDGERDALLRYRQKADHQLGWQAPVWLW
                     LTDNATGARQDAKAAATGVIFGPEGTVNGADEAFSTLALHLQKAGMAQILNEPADFGL
                     LQLSLRLRQELKASL"
BASE COUNT           79 a           97 c          108 g           68 t
ORIGIN      
        1 caaccggttg atggcatcat tcaggtgatg agtatttcag ccctcctgac ggatggcgaa
       61 cgggatgccc tcctgcgtta ccgtcagaaa gccgaccatc agttgggctg gcaggcacca
      121 gtctggctgt ggctgactga caacgctacc ggtgcccggc aggacgcgaa agcggccgca
      181 acaggggtca tcttcggacc ggaaggtacc gtgaacggtg cggacgaagc gttttccaca
      241 ctggcgttac acctgcaaaa agcaggaatg gcgcagatac tgaatgaacc tgctgatttc
      301 ggtctccttc agttgtcact cagactgcgg caggagctga aagccagcct gg
//
LOCUS       DAZKFU020000842          352 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.853, whole genome shotgun sequence.
ACCESSION   DAZKFU020000842 DAZKFU020000000
VERSION     DAZKFU020000842.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.853"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(65..>352)
                     /locus_tag="ACHOQQ_RS27310"
                     /old_locus_tag="pgaptmp_005462"
     CDS             complement(65..>352)
                     /locus_tag="ACHOQQ_RS27310"
                     /old_locus_tag="pgaptmp_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4473552.1"
                     /translation="RISEIVGFLYKNNISDTNTLYFSSPEALRFLWGVVDSPVYDIRH
                     LTERCSANDISHLYRAFMSVEKLTLSGRQVNVLMMLLYGSNGTEGARYEVA"
BASE COUNT           96 a           90 c           68 g           98 t
ORIGIN      
        1 tttttctgct cattattagc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcatat cgtgccccct ctgttccgtt actgccataa agcagcatca
      121 tcagtacatt tacctgccgg cctgacaggg tcagcttctc caccgacatg aatgcgcggt
      181 acagatgact gatatcgttt gctgaacatc gctcagtaag atgtctgata tcataaaccg
      241 gactgtcaac cactccccag agaaaacgga gcgcttccgg agaagaaaaa taaagggtgt
      301 tcgtatcact tatattattc ttatacagaa aaccgacaat ttcagatatc cg
//
LOCUS       DAZKFU020000843          351 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.854, whole genome shotgun sequence.
ACCESSION   DAZKFU020000843 DAZKFU020000000
VERSION     DAZKFU020000843.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.854"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..70)
                     /locus_tag="ACHOQQ_RS27315"
                     /old_locus_tag="pgaptmp_005463"
     CDS             complement(<1..70)
                     /locus_tag="ACHOQQ_RS27315"
                     /old_locus_tag="pgaptmp_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473553.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIF"
     gene            complement(137..>351)
                     /locus_tag="ACHOQQ_RS27320"
                     /old_locus_tag="pgaptmp_005464"
     CDS             complement(137..>351)
                     /locus_tag="ACHOQQ_RS27320"
                     /old_locus_tag="pgaptmp_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473554.1"
                     /translation="SLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSPII
                     PLNWSKASMTLMLKEMSNLINDEGIK"
BASE COUNT           92 a           79 c           65 g          115 t
ORIGIN      
        1 agaagataaa tattaaagtc gatgtcacaa ccagacctat caggtaagct aacagtaatt
       61 tattgatcac ttttacctcc cctttcgatt tctctttgag cggtcctttt aatccaggac
      121 cgcctcatta tgaaaactat ttaattccct catcattgat aaggttggac atctctttca
      181 acatgagagt cattgaagct ttcgaccaat ttagcggaat gatagggctt ggcgcttcat
      241 gcaaagttcc cgacttgtgg atatagttat agctttcggg aagtgccatt tctggtacgc
      301 tcctgccatt tgcaccaatc atattctcgc ctgttatttg ggccaatgat c
//
LOCUS       DAZKFU020000844          350 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.855, whole genome shotgun sequence.
ACCESSION   DAZKFU020000844 DAZKFU020000000
VERSION     DAZKFU020000844.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.855"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>350)
                     /locus_tag="ACHOQQ_RS27325"
                     /old_locus_tag="pgaptmp_005465"
     CDS             complement(<1..>350)
                     /locus_tag="ACHOQQ_RS27325"
                     /old_locus_tag="pgaptmp_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU4473555.1"
                     /translation="AELLDKLTKITDARLCKGFSDWASSVKEGASNDVKENVDRALLR
                     MFKCVELHNNELDLSYLFWGSVPPLPEWIEMLYLSDNQLDSVQVPESCKELGLDSNNL
                     TEFPQVPDGITLIS"
BASE COUNT           98 a           76 c           70 g          106 t
ORIGIN      
        1 ggagatcagg gtaattccat caggtacttg tggaaattct gtaaggttat tggaatcgag
       61 tcctaattct ttgcatgatt cgggcacctg tactgagtca agttgattat cactaaggta
      121 aagcatttca atccactctg gaagaggcgg tacagaaccc caaaataggt atgataagtc
      181 aagttcatta ttgtggagct caacacattt aaacattctc agcaatgccc tatccacatt
      241 ttctttgacg tcattcgatg cgccttcttt gactgaacta gcccagtcac tgaaaccttt
      301 gcaaagcctg gcatctgtaa ttttggttaa cttgtccagc aactctgcaa
//
LOCUS       DAZKFU020000845          349 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.856, whole genome shotgun sequence.
ACCESSION   DAZKFU020000845 DAZKFU020000000
VERSION     DAZKFU020000845.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.856"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>349
                     /locus_tag="ACHOQQ_RS27330"
                     /old_locus_tag="pgaptmp_005466"
     CDS             <1..>349
                     /locus_tag="ACHOQQ_RS27330"
                     /old_locus_tag="pgaptmp_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HGU4473556.1"
                     /translation="PEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLK
                     RASEVVIATDADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQT
                     ALLSEAGKGRARAD"
BASE COUNT           82 a           73 c          115 g           79 t
ORIGIN      
        1 ccggaagctt acggtgaaca gttcggttcc ccatggcgtt ctgatgtgtt gccgctgata
       61 ccggaacaat ggcagatggt ggtgaagccg aaggtgaaaa aacaacttat tgtgatccgg
      121 gggctgttaa aacgcgcttc tgaagtggtt attgccacag atgccgatcg tgaaggagaa
      181 gtgatagcca gagagttgct ggagtactgt cagtacagag ggcgtgtcag gcgtctgtgg
      241 ttatctgcac tggatgaggc cagtatcagg aaggcgctga ataatattct cccgggcgaa
      301 cagactgcgc ttctcagtga agccggtaag gggcgtgccc gggctgact
//
LOCUS       DAZKFU020000846          348 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.857, whole genome shotgun sequence.
ACCESSION   DAZKFU020000846 DAZKFU020000000
VERSION     DAZKFU020000846.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.857"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..224
                     /locus_tag="ACHOQQ_RS27335"
                     /old_locus_tag="pgaptmp_005467"
     CDS             <1..224
                     /locus_tag="ACHOQQ_RS27335"
                     /old_locus_tag="pgaptmp_005467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473557.1"
                     /translation="ASRRNVAGGKYPRTLVMNGCFYISCFLMNCVSVVNIHGGKFSGQ
                     KNPPVSSGSTASCFWWKWYGYEVLLLCMP"
BASE COUNT           91 a           64 c           83 g          110 t
ORIGIN      
        1 ccgccagtcg caggaatgtc gctggcggta aatacccacg aacccttgta atgaatggat
       61 gtttttacat cagttgtttt ttaatgaatt gcgtttctgt tgtcaacatt catggtggga
      121 agttttcagg acaaaaaaat ccaccagtaa gctcagggag tactgcgtca tgcttctggt
      181 ggaaatggta cggctatgag gttttattgt tatgcatgcc ataatttttc tacacaaaag
      241 caattctgaa tgtcaatacg ttttataagg aattgttgtg cacacagagt aaatacacgg
      301 ggctgtcagt gtaacactgg cggccttttt catgttgtga gcttccgg
//
LOCUS       DAZKFU020000847          347 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.858, whole genome shotgun sequence.
ACCESSION   DAZKFU020000847 DAZKFU020000000
VERSION     DAZKFU020000847.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.858"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..213
                     /gene="yhhH"
                     /locus_tag="ACHOQQ_RS27340"
                     /old_locus_tag="pgaptmp_005468"
     CDS             <1..213
                     /gene="yhhH"
                     /locus_tag="ACHOQQ_RS27340"
                     /old_locus_tag="pgaptmp_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HGU4473558.1"
                     /translation="KAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHS
                     DQIAGGVFIIEIGKNDGRILNFGHGK"
BASE COUNT          129 a           50 c           86 g           82 t
ORIGIN      
        1 aaagctatag cattggaatt agctgagata tatgttaaga atcgttatgg acaggatgcc
       61 gcagaagaag aaaaaccata cgaaatcact gagttaacaa caagttgggt tgttgaaggt
      121 accattcact cagaccaaat tgctggtggg gtttttatta tagaaatagg caaaaatgat
      181 gggagaattc tgaattttgg ccacggaaaa taagtatgta aggataatca aacatcgtgt
      241 gcgctgatgg cagagtaggt ggaggactcc agacagtcaa acgatagaaa aagatagcct
      301 ttatggaggt tcctgcaatg tcaaatacat accagaaaag aaaggca
//
LOCUS       DAZKFU020000848          347 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.859, whole genome shotgun sequence.
ACCESSION   DAZKFU020000848 DAZKFU020000000
VERSION     DAZKFU020000848.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.859"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>347
                     /locus_tag="ACHOQQ_RS27345"
                     /old_locus_tag="pgaptmp_005469"
     CDS             <1..>347
                     /locus_tag="ACHOQQ_RS27345"
                     /old_locus_tag="pgaptmp_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473559.1"
                     /translation="EKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYGVL
                     MLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVLTA
                     SWKEQLENLPEML"
BASE COUNT           88 a           79 c          115 g           65 t
ORIGIN      
        1 gtgagaaaca gccgcagata ccggagggtg cgtctggtat gtcgccggag cgaagaaaaa
       61 gtcgtttccc cggcgtgttg atactgtctg tcactgtggg gctggtcatg ggggcagtgg
      121 tatatggcgt actgatgctt atgaccggtc ttgacagccg gatcacgacc ctggaaggcc
      181 agagaagaga aatgctttct gcgtcagaac tcgcgcccct gagaatgaaa ctgacagaga
      241 cggagaagca actgaaggat atggaaaagc gcctggcagt cctggagcag cagcccgtgc
      301 tgacagcatc ctggaaggaa caactggaga atctgccgga gatgctg
//
LOCUS       DAZKFU020000849          346 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.860, whole genome shotgun sequence.
ACCESSION   DAZKFU020000849 DAZKFU020000000
VERSION     DAZKFU020000849.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.860"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>346
                     /locus_tag="ACHOQQ_RS27350"
                     /old_locus_tag="pgaptmp_005470"
     CDS             <1..>346
                     /locus_tag="ACHOQQ_RS27350"
                     /old_locus_tag="pgaptmp_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT protein"
                     /protein_id="HGU4473560.1"
                     /translation="YNIIYELPSHLLLKEYAYTKVSEKIDSTFELQIRSILSEGWHEV
                     EHDLRYKAQYDWDGHDTESRKLNGIYATLETSEWTMIKIFDELAYKHYKNKNWNGMLR
                     QKLRLRLGTQELH"
BASE COUNT          131 a           52 c           69 g           94 t
ORIGIN      
        1 tacaatataa tatatgaact accttctcac ttacttctaa aagaatacgc atatacaaag
       61 gtttctgaaa aaatagattc aaccttcgag ttacaaataa gatctatctt atccgaagga
      121 tggcatgaag tcgagcatga cctacgatat aaggcgcaat atgactggga tggtcatgac
      181 actgagagca ggaagttgaa tggtatatat gcaacgttag aaacgtctga atggacaatg
      241 atcaagattt ttgatgagtt agcatataaa cattataaaa ataaaaactg gaatggtatg
      301 cttcggcaga aattacgatt aaggttaggt acgcaagagt tacatc
//
LOCUS       DAZKFU020000850          346 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.861, whole genome shotgun sequence.
ACCESSION   DAZKFU020000850 DAZKFU020000000
VERSION     DAZKFU020000850.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.861"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            173..346
                     /locus_tag="ACHOQQ_RS27355"
                     /old_locus_tag="pgaptmp_005471"
                     /pseudo
     CDS             173..346
                     /locus_tag="ACHOQQ_RS27355"
                     /old_locus_tag="pgaptmp_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          108 a           74 c           92 g           72 t
ORIGIN      
        1 tcaggtataa acacagctgg attgagaata aattcaaaga aaaaataatt gaataaccat
       61 gttatgtata gacattttag atggaacgaa ccagaggaag gcatgcctcc gacttgggtt
      121 atatgggcac ggagttttag tgtagtgaac tcaacgacgc aaaccgaata acctgccgct
      181 ggcgcttatc agcacggaag gggcaacaga atggtgcgca gaatacgatg agtggggcaa
      241 cctgctgaat gaagagaacc cgcatcagct gcagcagctt atccgcctgc cggggcagca
      301 gtatgatgag gagtccggcc tgtattacaa ccgccaccgc tattat
//
LOCUS       DAZKFU020000851          345 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.862, whole genome shotgun sequence.
ACCESSION   DAZKFU020000851 DAZKFU020000000
VERSION     DAZKFU020000851.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.862"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..299
                     /gene="iraP"
                     /locus_tag="ACHOQQ_RS27360"
                     /old_locus_tag="pgaptmp_005472"
     CDS             39..299
                     /gene="iraP"
                     /locus_tag="ACHOQQ_RS27360"
                     /old_locus_tag="pgaptmp_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGU4473561.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          109 a           65 c           85 g           86 t
ORIGIN      
        1 tgttaacttc tccatacttt ggataaggaa atacagacat gaaaaatctc attgctgagt
       61 tgttatttaa gcttgcccaa aaagaagaag agtcgaaaga actgtgtgcg caggtagaag
      121 ctttggagat tatcgtcact gcaatgcttc gcaatatggc gcaaaatgac caacaacggt
      181 tgattgatca ggtagagggg gcgctgtacg aggtaaagcc cgatgccagc attcctgacg
      241 acgatacgga gctgctgcgc gattacgtaa agaagttatt gaagcatcct cgtcagtaaa
      301 aagttaatct tttcaacaga tgtcataaag ttgtcacggc cgaga
//
LOCUS       DAZKFU020000852          342 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.863, whole genome shotgun sequence.
ACCESSION   DAZKFU020000852 DAZKFU020000000
VERSION     DAZKFU020000852.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.863"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            109..>342
                     /locus_tag="ACHOQQ_RS27365"
                     /old_locus_tag="pgaptmp_005473"
     CDS             109..>342
                     /locus_tag="ACHOQQ_RS27365"
                     /old_locus_tag="pgaptmp_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HGU4473562.1"
                     /translation="MARLSLSREGRDSLERYMIQQASLAGITTERLGKTFSVDPSIQQ
                     KMENAIKESAGLLQEINFIGVDDQEGEKVLIDTS"
BASE COUNT           98 a           82 c           91 g           71 t
ORIGIN      
        1 actgaaagcg caactgagaa accaggatgc cgatccacag cagcgtttta cttccgctgg
       61 cggcactgac cagacactgg ctgaattctg acaggagaag agtaatttat ggcccgttta
      121 tccctttccc gtgaaggacg tgacagtctg gaacgctata tgattcagca ggcatcgctg
      181 gctggtatca caacagagcg cctgggtaaa accttcagcg tggatccatc cattcagcag
      241 aagatggaga acgccatcaa ggaaagcgcc ggactgttac aggaaatcaa ttttatcggt
      301 gtggatgacc aggaaggtga aaaagtcctg attgacacca gc
//
LOCUS       DAZKFU020000853          340 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.864, whole genome shotgun sequence.
ACCESSION   DAZKFU020000853 DAZKFU020000000
VERSION     DAZKFU020000853.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.864"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..193)
                     /locus_tag="ACHOQQ_RS27370"
                     /old_locus_tag="pgaptmp_005474"
     CDS             complement(<1..193)
                     /locus_tag="ACHOQQ_RS27370"
                     /old_locus_tag="pgaptmp_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473563.1"
                     /translation="MNLKKIFFSAVTVSVLCTLTGCDYIEEGKPESSLLKQQEEHNNK
                     IELIEKQQAQLKSQLETIQK"
BASE COUNT           88 a           58 c           60 g          134 t
ORIGIN      
        1 gtttttgaat agtttccagt tgactcttta gctgtgcttg ttgtttttca attagctcaa
       61 ttttattgtt atgttcttct tgttgtttca gtaaactact ttctggcttg ccttcttcaa
      121 tgtagtcaca tcccgttaat gtgcagagaa ctgaaacagt gacagcactg aaaaaaatct
      181 tcttcaagtt catgtaaaaa tccttattgc ctccgaccag cgatcaatgc cggtagagtt
      241 gtccattgta ttattggtga tctatgttgc tttacattaa tgacagtgat gggtgtcgat
      301 taaatattgt tttaagcatt catcataaaa atgtatcatt
//
LOCUS       DAZKFU020000854          337 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.865, whole genome shotgun sequence.
ACCESSION   DAZKFU020000854 DAZKFU020000000
VERSION     DAZKFU020000854.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.865"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>337
                     /locus_tag="ACHOQQ_RS27375"
                     /old_locus_tag="pgaptmp_005475"
     CDS             <1..>337
                     /locus_tag="ACHOQQ_RS27375"
                     /old_locus_tag="pgaptmp_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HGU4473564.1"
                     /translation="ASRETVSRVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPV
                     TCRDAEILDPRYLDMVADACMSRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARI
                     GSAQTPGQMA"
BASE COUNT           83 a           77 c          113 g           64 t
ORIGIN      
        1 gcctccaggg agactgtcag ccgggtgctg ctgatgctgc ggatcacggg ctggctgagt
       61 ctgtgcaaac gggtgcgtga tgagaaaggg cgggtacggg gcaatattta tgcccagcac
      121 gatgaaccgg tgacgtgccg ggatgctgaa atactggatc cacgttatct ggatatggtg
      181 gcagatgcct gtatgagccg gaaccggacc ataagtcaga cggcccggga tattctgtgc
      241 gaaataaaaa atgatccggg aatgcgtcat tatcacagcc atatcagtca actggaagcc
      301 cggataggga gtgcccagac accgggacag atggcgg
//
LOCUS       DAZKFU020000855          333 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.866, whole genome shotgun sequence.
ACCESSION   DAZKFU020000855 DAZKFU020000000
VERSION     DAZKFU020000855.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.866"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            173..333
                     /locus_tag="ACHOQQ_RS27380"
                     /old_locus_tag="pgaptmp_005476"
                     /pseudo
     CDS             173..333
                     /locus_tag="ACHOQQ_RS27380"
                     /old_locus_tag="pgaptmp_005476"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          104 a           58 c           74 g           97 t
ORIGIN      
        1 ctgggagtta gtaataaaaa tggttataca aaataaaaac tccgttggaa catcgtatat
       61 gaattaaaaa cttcgagttg ttttagttga gtaggtacta tgcatcatat ccgtgtagta
      121 cctccataac gggtagtatg atagacctat ttagcaagtt tgtagtcctg acccgatagg
      181 gctaaatgga ggtatcaacc tctatgctta tgcgccgaat ccgctgggtt ggatcgatcc
      241 gcttggatta aatacggaac ctacgttacc cccaggaact attattaaag atggtggagt
      301 aacaatagag cattattatc atggcagtga tca
//
LOCUS       DAZKFU020000856          332 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.867, whole genome shotgun sequence.
ACCESSION   DAZKFU020000856 DAZKFU020000000
VERSION     DAZKFU020000856.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.867"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>332
                     /locus_tag="ACHOQQ_RS27385"
                     /old_locus_tag="pgaptmp_005477"
     CDS             <1..>332
                     /locus_tag="ACHOQQ_RS27385"
                     /old_locus_tag="pgaptmp_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HGU4473565.1"
                     /translation="TGVCLPPFADAHQLVEQGETLQSVDEHSLPDLDEDEGDEEEEKR
                     DILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMATHTTYREGRQMLPVDLQS
                     ALWEISRDT"
BASE COUNT           80 a           72 c          120 g           60 t
ORIGIN      
        1 acgggggtgt gtcttcctcc atttgcggat gctcatcaac tggtggagca gggagagacg
       61 ctgcagagtg tggatgagca ttcactgccc gatcttgatg aagatgaggg ggatgaagag
      121 gaagagaaac gcgacattct gggggagatg gaaatctctg cccgcatgat gatcaccgga
      181 ggggatccga aagaagaagc agcgctcaaa cgtgcggaca gggcgatgat ccgtgaggca
      241 ctgctgatgg ccacgcacac cacgtaccgg gaggggcgtc agatgctgcc ggtggatctg
      301 cagagtgcgt tgtgggaaat ttcgcgggac ac
//
LOCUS       DAZKFU020000857          332 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.868, whole genome shotgun sequence.
ACCESSION   DAZKFU020000857 DAZKFU020000000
VERSION     DAZKFU020000857.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.868"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..216)
                     /gene="ynfN"
                     /locus_tag="ACHOQQ_RS27390"
                     /old_locus_tag="pgaptmp_005478"
     CDS             complement(<1..216)
                     /gene="ynfN"
                     /locus_tag="ACHOQQ_RS27390"
                     /old_locus_tag="pgaptmp_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HGU4473566.1"
                     /translation="MKKIIVFFNSEPAMVVPVMTGVNTIMREYPNGETTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAM"
     gene            complement(248..286)
                     /locus_tag="ACHOQQ_RS27395"
                     /old_locus_tag="pgaptmp_005479"
                     /pseudo
     CDS             complement(248..286)
                     /locus_tag="ACHOQQ_RS27395"
                     /old_locus_tag="pgaptmp_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           94 a           71 c           61 g          106 t
ORIGIN      
        1 cattgctgct tccagaattt cttctgaagt tacatgtcga tccgctgcta cataaatgac
       61 tttatgatct ccggtcagag atggaaaccc tgcagccatt acagtgaggt gtgttgtttc
      121 gccatttgga tattcacgca tgatggtgtt aactccggtc atcactggca ctaccattgc
      181 tggttcagag ttaaaaaaaa ctattatttt tttcataatg ttaccgtaat atgtgagtat
      241 ccatcgacta gacactaagc aaaaaagctc ccgaaggagc cttcattttc actttcttaa
      301 atctaacgac agacggctaa catttaagta tt
//
LOCUS       DAZKFU020000858          331 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.869, whole genome shotgun sequence.
ACCESSION   DAZKFU020000858 DAZKFU020000000
VERSION     DAZKFU020000858.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.869"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(11..>331)
                     /locus_tag="ACHOQQ_RS27400"
                     /old_locus_tag="pgaptmp_005480"
     CDS             complement(11..>331)
                     /locus_tag="ACHOQQ_RS27400"
                     /old_locus_tag="pgaptmp_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4473567.1"
                     /translation="NYSGRASRREFWWYYLFNLIFLGVLFFIGYTLDNTGISYGFYIP
                     CVYVLATIIPSITLKVRRLHDIGLSAWYLFVWCIPYIGGFILFVMYCIPSEKGDNKYG
                     AYSI"
BASE COUNT          125 a           71 c           52 g           83 t
ORIGIN      
        1 ctggcgggga ttaaatacta tatgcaccgt atttattatc ccctttttct gacgggatac
       61 agtacataac gaacaatata aagccaccaa tatacggaat gcaccagaca aacaagtacc
      121 aggcacttaa cccaatatca tggagtctgc ggactttcaa tgtaatggat ggaataatcg
      181 tagctaatac gtaaacgcaa ggtatataaa acccataact aatgccagta ttatctaatg
      241 tatatccgat aaaaaaaaga actcccaaga atattaaatt aaacagataa taccaccaga
      301 actctcttcg actggctcgc cctgaataat t
//
LOCUS       DAZKFU020000859          330 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.870, whole genome shotgun sequence.
ACCESSION   DAZKFU020000859 DAZKFU020000000
VERSION     DAZKFU020000859.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.870"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>330
                     /locus_tag="ACHOQQ_RS27405"
                     /old_locus_tag="pgaptmp_005481"
     CDS             <1..>330
                     /locus_tag="ACHOQQ_RS27405"
                     /old_locus_tag="pgaptmp_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473568.1"
                     /translation="ILHGCPAESIELIKNAKRTGQWSYEMTDKNGQNIEVSYIFTDTI
                     DDKSDGKLCLKICNSCNQNDSSTHEYKIDHQQLKNHIRQLEEWALLEKFYDNNAYYSK
                     RTKQLEKK"
BASE COUNT          126 a           54 c           61 g           89 t
ORIGIN      
        1 attcttcatg gttgtcctgc agagtccatc gaactcatta agaatgcgaa acgaacgggg
       61 caatggtcat atgagatgac tgataagaac gggcagaata ttgaagtgtc ctatattttc
      121 actgatacca tagatgataa atctgatgga aaattatgct taaaaatctg taactcctgc
      181 aaccaaaatg attctagtac acatgaatat aaaatagatc atcaacagtt gaaaaatcac
      241 ataaggcaac ttgaggagtg ggcccttctt gaaaagtttt atgataacaa cgcttattat
      301 tctaaacgta caaaacaatt agagaagaaa
//
LOCUS       DAZKFU020000860          330 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.871, whole genome shotgun sequence.
ACCESSION   DAZKFU020000860 DAZKFU020000000
VERSION     DAZKFU020000860.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.871"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          110 a           70 c           60 g           90 t
ORIGIN      
        1 tataacataa caataagata cattctcagc tagatcattt caacagaatc cccgagccca
       61 agggtaatgt ggcatcgcag cctctcatca aatggaagac atgtgattgc acatttatca
      121 tacaagacgt atgataacac tgtgttattt atcctgctgc tgagggtata gatatgaata
      181 acgaatctga aaaagcaatg tggcgttgta agcctgtgga taatccatgg accttcactt
      241 ccctgctgaa aaaagcagtc tgtgccgttt taccagattt cacacctgct caagataaac
      301 cccaaaatat gttttcacta agtaatgaaa
//
LOCUS       DAZKFU020000861          328 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.872, whole genome shotgun sequence.
ACCESSION   DAZKFU020000861 DAZKFU020000000
VERSION     DAZKFU020000861.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.872"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>328
                     /locus_tag="ACHOQQ_RS27410"
                     /old_locus_tag="pgaptmp_005482"
     CDS             <1..>328
                     /locus_tag="ACHOQQ_RS27410"
                     /old_locus_tag="pgaptmp_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4473569.1"
                     /translation="IWLCVLTALGISALNNRDLIRQVSTDLQRWNAVPKGHYRPEAES
                     LAALKQDALLLEQWQRQGVPLRYSLGYYPGQRLWLALQQAIDTRVPPPSPEPEPVPEI
                     VRLDSLA"
BASE COUNT           65 a           94 c           97 g           72 t
ORIGIN      
        1 tgatatggct gtgtgtcctg actgcgctgg ggatttctgc gcttaacaat cgtgatctga
       61 tccgccaggt aagcaccgat ttacagcgct ggaatgctgt cccgaagggg cactaccgtc
      121 ctgaggcgga atcgctggcc gcactgaaac aggatgcgct tctgcttgag cagtggcagc
      181 gtcagggagt gcctttacgt tacagcctgg gctattaccc gggacaacgt ctgtggctgg
      241 ccctgcagca ggctatcgat acccgggttc caccaccatc ccctgaacca gaaccggtgc
      301 cggaaattgt acgactcgat agcctggc
//
LOCUS       DAZKFU020000862          326 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.873, whole genome shotgun sequence.
ACCESSION   DAZKFU020000862 DAZKFU020000000
VERSION     DAZKFU020000862.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.873"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           89 a           98 c           66 g           73 t
ORIGIN      
        1 gcctggtttg attattgtcg cagttatttt atatcgcttt cccatactgg ctctcatctc
       61 aacaacaaaa tcaccggctg agccatcact ccggtaatgc atgcgaaaaa aagtgagcgt
      121 ctcagcaatg gctcatgcgg tgattctgta cggtacccgc acaaaatacg ggcaccaaca
      181 gcgtcgcaga atacaacgaa ggcatcaggg cagcacctct ctcccgctat ttttcagagt
      241 tctttaccct cctcctgaca cgggagtaca accctgacag catccgggcc agtacattcg
      301 caagcaccca tgaaaacaca gccaca
//
LOCUS       DAZKFU020000863          325 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.874, whole genome shotgun sequence.
ACCESSION   DAZKFU020000863 DAZKFU020000000
VERSION     DAZKFU020000863.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.874"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          115 a           65 c           65 g           80 t
ORIGIN      
        1 cagtctccat taacgaataa agagaaaatg gagactttta tattcccaaa agagagaata
       61 ggattgtagc gaattttaac caaatagaca aacacggctc agcatatact ggtgatatgg
      121 gaagaggatg attgcgagat taccctatcc taatttacag aataagagat tatatacaga
      181 taaaaaagcc tcatactcat cacgtactgt ttttgtatga cgcaacaata tcagcgaacg
      241 agccattttc cgccagccac aaagcctgac gcatctacct gaattgacgg atgagcaaac
      301 tggctggact ctgtagtccg gaatt
//
LOCUS       DAZKFU020000864          322 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.875, whole genome shotgun sequence.
ACCESSION   DAZKFU020000864 DAZKFU020000000
VERSION     DAZKFU020000864.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.875"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            66..278
                     /gene="cspI"
                     /locus_tag="ACHOQQ_RS27415"
                     /old_locus_tag="pgaptmp_005483"
     CDS             66..278
                     /gene="cspI"
                     /locus_tag="ACHOQQ_RS27415"
                     /old_locus_tag="pgaptmp_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HGU4473570.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSVIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT           95 a           58 c           68 g          101 t
ORIGIN      
        1 ttctctctga tatttttttt cggaccattc tgcccaaggg ctaacttctt caaaaggtaa
       61 taatgatgtc taacaaaatg actggtttag tgaaatggtt taaccctgaa aaaggttttg
      121 gtttcatcac gccgaaagat ggtagcaaag atgtgtttgt ccatttctca gttattcaga
      181 gcaacgattt caaaacgtta actgagaatc aggaagtcga atttggtatt gagaacggac
      241 ctaaaggccc cgcggctgtt catgtagtgg cactttgagg tagaaaatat tacaaaccat
      301 attcacttta gatgcccgtg tt
//
LOCUS       DAZKFU020000865          314 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.876, whole genome shotgun sequence.
ACCESSION   DAZKFU020000865 DAZKFU020000000
VERSION     DAZKFU020000865.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.876"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(102..>314)
                     /locus_tag="ACHOQQ_RS27420"
                     /old_locus_tag="pgaptmp_005484"
     CDS             complement(102..>314)
                     /locus_tag="ACHOQQ_RS27420"
                     /old_locus_tag="pgaptmp_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4473571.1"
                     /translation="ISLLFPVINDNTWLQETFGGAFSMWIFVVFNLVCYVFISRYVPE
                     TKGIPLTQIEQLAGNKLREIQGKAMM"
BASE COUNT          106 a           70 c           50 g           88 t
ORIGIN      
        1 atttattatc tccttttgtt acctatatta acacaaggaa aatcccggta agatgctcaa
       61 cattaccggg cattactgct gattgtgaca caatcagact attacatcat agctttaccc
      121 tgaatttcac gcagtttatt tccggcaagc tgctcaattt gtgttagcgg gatccccttt
      181 gtttcaggaa catagcgaga aataaaaaca taacaaacca gattaaagac aacaaaaatc
      241 cacatagaaa acgctccgcc aaaggtttcc tgcagccagg tattatcatt tattaccgga
      301 aacaacagtg atat
//
LOCUS       DAZKFU020000866          312 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.877, whole genome shotgun sequence.
ACCESSION   DAZKFU020000866 DAZKFU020000000
VERSION     DAZKFU020000866.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.877"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(124..>312)
                     /locus_tag="ACHOQQ_RS27425"
                     /old_locus_tag="pgaptmp_005485"
     CDS             complement(124..>312)
                     /locus_tag="ACHOQQ_RS27425"
                     /old_locus_tag="pgaptmp_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473572.1"
                     /translation="NNSISVTPVINGQTQAAFNLAKCPAAGLDNDKLLITDITSNQDT
                     YPVLIEYVTVDINNSNAA"
BASE COUNT           65 a           80 c           70 g           97 t
ORIGIN      
        1 cataacgatg tctggcgttt taaaaaaaat gaataacaat aataaacact ctccataatt
       61 cctcttgcgc tcccgtcagg tatcttctcc tctaagtctt gcctgccggg agcctctttt
      121 ttcttatgcg gcgttgctgt tattgatatc caccgtcaca tactcaatca gcacggggta
      181 ggtgtcctgg ttactggtga tatccgtgat cagcagtttg tcgttatcca gtcctgccgc
      241 cgggcatttc gccagattaa aggccgcctg ggtctgaccg ttgatgaccg gtgtcacgct
      301 gatgctgtta tt
//
LOCUS       DAZKFU020000867          311 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.878, whole genome shotgun sequence.
ACCESSION   DAZKFU020000867 DAZKFU020000000
VERSION     DAZKFU020000867.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.878"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>311
                     /locus_tag="ACHOQQ_RS27430"
                     /old_locus_tag="pgaptmp_005486"
     CDS             <1..>311
                     /locus_tag="ACHOQQ_RS27430"
                     /old_locus_tag="pgaptmp_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU4473573.1"
                     /translation="LGFIPLSYYKNRDYACFFSANSTQKPALYDTPDATANSRINARL
                     PYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNNWIRGLVTEMTDPGDELQASHPL
                     R"
BASE COUNT           67 a           90 c           79 g           75 t
ORIGIN      
        1 ctgggcttta ttccgctctc ttactacaag aaccgggatt atgcctgctt cttctccgct
       61 aactccaccc agaaaccggc actgtacgat acgccggatg caacggcgaa cagccgtatt
      121 aatgcccgtc ttccctatat cttcctgctg tcccgcattg cgcattacct gaagatgatt
      181 cagcgggaaa atatcggtac caccaaagat cgccgtctgc ttgagctgga gctgaataac
      241 tggatccggg gtctggtgac ggagatgacc gatccgggtg atgagttgca ggcctcgcat
      301 ccgctgcgtg a
//
LOCUS       DAZKFU020000868          311 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.879, whole genome shotgun sequence.
ACCESSION   DAZKFU020000868 DAZKFU020000000
VERSION     DAZKFU020000868.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.879"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>311)
                     /locus_tag="ACHOQQ_RS27435"
                     /old_locus_tag="pgaptmp_005487"
     CDS             complement(<1..>311)
                     /locus_tag="ACHOQQ_RS27435"
                     /old_locus_tag="pgaptmp_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGU4473574.1"
                     /translation="HLPDKDEMRGVPPADALRAVQLLWNEMSNPGGTFPVTHDTYLKL
                     FQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVVLVGDCYQQIYRFRGANNAL
                     S"
BASE COUNT           75 a           98 c           78 g           60 t
ORIGIN      
        1 tgggaaagcg cattattcgc accccgaaag cggtaaatct gttgataaca atctccaaca
       61 aggaccaccc gacacttctg ccccagaacc agagcagtgg tcaccggatt cgcatcctgc
      121 gcctcatcaa acaggatggt gtcccatcgg tgagacaggt ctgcaccgga caactggaag
      181 agtttcagat aagtatcgtg cgtcaccggg aacgtccccc cggggttgct catttcgttc
      241 cagagcaact gcaccgcccg aagcgcatct gctggcggaa caccccgcat ctcatcctta
      301 tccggcaggt g
//
LOCUS       DAZKFU020000869          308 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.880, whole genome shotgun sequence.
ACCESSION   DAZKFU020000869 DAZKFU020000000
VERSION     DAZKFU020000869.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.880"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..>308
                     /locus_tag="ACHOQQ_RS27440"
                     /old_locus_tag="pgaptmp_005488"
     CDS             24..>308
                     /locus_tag="ACHOQQ_RS27440"
                     /old_locus_tag="pgaptmp_005488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473575.1"
                     /translation="MSLADKNEPERLYMESKGIVRDMWNAMVYLREAYSERVKSLDWY
                     TPENSFLNTQEPELIAIPEFNESEAENDKISRSIINWPYLDAVNKKDIRGF"
BASE COUNT          109 a           43 c           68 g           88 t
ORIGIN      
        1 acaatagcat aagcacagga tatatgtcat tagctgataa aaatgaaccc gaaagattat
       61 atatggagtc aaaaggaatt gttcgtgaca tgtggaatgc tatggtttat ttgagggaag
      121 cttattctga gagggttaag tctctggact ggtatacacc agaaaactca ttcttaaata
      181 cgcaagaacc tgaacttatt gcaattccag agttcaatga aagcgaggcg gaaaatgata
      241 aaatttccag gtcgattatt aactggcctt atcttgatgc tgtgaataag aaagatataa
      301 gaggtttt
//
LOCUS       DAZKFU020000870          308 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.881, whole genome shotgun sequence.
ACCESSION   DAZKFU020000870 DAZKFU020000000
VERSION     DAZKFU020000870.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.881"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>308
                     /locus_tag="ACHOQQ_RS27445"
                     /old_locus_tag="pgaptmp_005489"
     CDS             <1..>308
                     /locus_tag="ACHOQQ_RS27445"
                     /old_locus_tag="pgaptmp_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4473576.1"
                     /translation="FLISYRPNWRWLGMRETAAKSFVDEVEAAWSEYAEGMSGEIDVE
                     GKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQLFRTRFKAVSPKRVDTPGHGMGNR
                     "
BASE COUNT           68 a           65 c          108 g           67 t
ORIGIN      
        1 tttctgatca gctaccgtcc gaactggcgc tggctgggga tgcgggagac tgcggcaaaa
       61 agttttgtcg atgaggtgga ggcggcctgg tcggaatacg ccgaagggat gtctggcgag
      121 atcgacgtgg aaggaaaacg cacgttcacg gaatttatcc gtgaaggtgt gggcgttcat
      181 gcgtttaacg gcgaaatctt tgtgcagccg gtctgggata cggagagcac gcagctgttt
      241 cgtacacgtt ttaaagccgt gagtccgaaa cgggtggaca cgccaggaca cggtatgggg
      301 aaccgttt
//
LOCUS       DAZKFU020000871          305 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.882, whole genome shotgun sequence.
ACCESSION   DAZKFU020000871 DAZKFU020000000
VERSION     DAZKFU020000871.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.882"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>305)
                     /locus_tag="ACHOQQ_RS27450"
                     /old_locus_tag="pgaptmp_005490"
     CDS             complement(<1..>305)
                     /locus_tag="ACHOQQ_RS27450"
                     /old_locus_tag="pgaptmp_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4473577.1"
                     /translation="LGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATNLNAMYHKKQ
                     NAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGAADKLQL"
BASE COUNT           62 a           94 c           74 g           75 t
ORIGIN      
        1 cagttgcagt ttgtccgccg cccctgatgc cggagatttc accagtgtga acggtgtacc
       61 ccgtttctca tccagtaccg gcgtcacctg aatgctgttg tttccggcaa actcaaaagc
      121 cagtgtgtgc catccgttat caaagacccc gaacgtatcc agcttcgcat tctgcttctt
      181 gtggtacatc gcgttcaggt tcgtcgcatc cgtctgcagg aagaaggaca tcagcatgtc
      241 gttgccttcc tctgacagcg tcactccctc cggcagggac gacaactgcc agtaaatacc
      301 caggg
//
LOCUS       DAZKFU020000872          305 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.883, whole genome shotgun sequence.
ACCESSION   DAZKFU020000872 DAZKFU020000000
VERSION     DAZKFU020000872.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.883"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            58..>305
                     /locus_tag="ACHOQQ_RS27455"
                     /old_locus_tag="pgaptmp_005491"
     CDS             58..>305
                     /locus_tag="ACHOQQ_RS27455"
                     /old_locus_tag="pgaptmp_005491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473578.1"
                     /translation="MARLSRVAYSSTQDRKKEQIAKIGEILPEGLGFPKIINLSYSAF
                     DSFQIPGCTFKEKKQLRQDILDGKGRYIYCGVRDVGAE"
BASE COUNT          100 a           46 c           74 g           85 t
ORIGIN      
        1 ctaccatgta gaatgtccgt aattgtaggc cggaacggtt gtgggaagag tacaacattg
       61 gcaagacttt ctagggttgc ttattcttca actcaggaca gaaaaaaaga acagatagcc
      121 aaaattggtg aaattcttcc agagggatta ggttttccga aaataatcaa cctttcatac
      181 agtgcttttg atagttttca aatacccggg tgtactttta aagaaaagaa acagctaagg
      241 caagacatac ttgatgggaa aggtaggtat atatattgcg gagtcaggga tgttggtgct
      301 gaatt
//
LOCUS       DAZKFU020000873          302 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.884, whole genome shotgun sequence.
ACCESSION   DAZKFU020000873 DAZKFU020000000
VERSION     DAZKFU020000873.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.884"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           90 a           64 c           54 g           94 t
ORIGIN      
        1 tcaatccatt ttacccagag tcataaaata gagtgtctgt tgatctcatt ttcatattcc
       61 tttcagactg aagtgcgttt tcatcacccg atgagaaaga actgaagtga tgtttgccat
      121 agattgcaag atgccctgtc tcattgtcta ttgtggtgca cctttatacc tgttatacca
      181 gatcaaaaat cacgcaatcc atacaacaaa accagatttg caattcgtgt cacaaaatat
      241 gtcgatcttt ttctaagaag aagatgccat gtgaagccag acgaacactt gcggtggtct
      301 tt
//
LOCUS       DAZKFU020000874          300 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.885, whole genome shotgun sequence.
ACCESSION   DAZKFU020000874 DAZKFU020000000
VERSION     DAZKFU020000874.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.885"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..200
                     /gene="ymgF"
                     /locus_tag="ACHOQQ_RS27460"
                     /old_locus_tag="pgaptmp_005492"
     CDS             <1..200
                     /gene="ymgF"
                     /locus_tag="ACHOQQ_RS27460"
                     /old_locus_tag="pgaptmp_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HGU4473579.1"
                     /translation="DYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTINLLNALV
                     WLAINLVNRLRERLVSHRDQQ"
BASE COUNT           76 a           62 c           69 g           93 t
ORIGIN      
        1 tggattattt cactctctat atcatctttt ccattgcatt tatgctgatc actctcctgg
       61 tcatccttat tgcaaaaccc agtaccgggc tgggagaagt gcttgtgacg ataaatttgc
      121 ttaatgccct tgtttggctg gcgatcaatc tggttaatcg attaagggaa agactcgtca
      181 gccacaggga tcagcaataa tctttcagtt tctcactgtc agtatgcggc tgaatggggt
      241 gctgacagtg aacgcctgga tcattgaagg aaaggaatta ttgcgcaaat agttgtcaac
//
LOCUS       DAZKFU020000875          297 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.886, whole genome shotgun sequence.
ACCESSION   DAZKFU020000875 DAZKFU020000000
VERSION     DAZKFU020000875.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.886"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..>297
                     /locus_tag="ACHOQQ_RS27465"
                     /old_locus_tag="pgaptmp_005493"
     CDS             14..>297
                     /locus_tag="ACHOQQ_RS27465"
                     /old_locus_tag="pgaptmp_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4473580.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINVVCDNGY
                     SLRVVDKSNQQSTSTPPPFTALAGIRCSTAHITAKDNAWLYSLSHQTSDTG"
BASE COUNT           77 a           82 c           75 g           63 t
ORIGIN      
        1 atctggaggc gaaatgatga aactggccct gacactggaa gccgacagcg ttaacgtaca
       61 ggcactgaac atggggcgca ttgtcgttga cattgatggt gttaatctcg ctgaactgat
      121 taacgtggtc tgcgataacg gttattcact ccgcgtggtc gataaatcca accaacagtc
      181 aaccagtaca ccaccaccgt ttacggccct tgctggcata cgctgcagta ccgcgcatat
      241 cacggcaaag gacaacgcct ggctgtactc gctgtcacat cagaccagtg acaccgg
//
LOCUS       DAZKFU020000876          297 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.887, whole genome shotgun sequence.
ACCESSION   DAZKFU020000876 DAZKFU020000000
VERSION     DAZKFU020000876.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.887"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>297
                     /locus_tag="ACHOQQ_RS27470"
                     /old_locus_tag="pgaptmp_005494"
     CDS             <1..>297
                     /locus_tag="ACHOQQ_RS27470"
                     /old_locus_tag="pgaptmp_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4473581.1"
                     /translation="AVTESYKPIWNWKISQYNVSDIKFEKIIKPQFADRTYFSNCSPV
                     DPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLL"
BASE COUNT           90 a           60 c           58 g           89 t
ORIGIN      
        1 tgcagttact gagtcgtaca aacccatctg gaactggaaa atatcgcaat acaatgtatc
       61 tgatattaaa ttcgaaaaaa ttataaaacc ccagttcgct gacagaacct atttctccaa
      121 ttgttcacct gttgatccca caagtaccag accaacctat tttggtgata ccgatggctc
      181 agttggtgca gttttgtacg cgctttttgc aacgggacat ttgggaatca tggctgaagg
      241 agaaaatttc cttagccagt tgcttaatat agaagatgaa gttctcaatg tattgct
//
LOCUS       DAZKFU020000877          296 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.888, whole genome shotgun sequence.
ACCESSION   DAZKFU020000877 DAZKFU020000000
VERSION     DAZKFU020000877.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.888"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>296
                     /locus_tag="ACHOQQ_RS27475"
                     /old_locus_tag="pgaptmp_005495"
     CDS             <1..>296
                     /locus_tag="ACHOQQ_RS27475"
                     /old_locus_tag="pgaptmp_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HGU4473582.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILT"
BASE COUNT           71 a           83 c           85 g           57 t
ORIGIN      
        1 atggcacata aaccactgga tctcggggcg gctattatgc agcccggtcg tcagaccact
       61 ggtgccggca atgtggtggc aatgggggaa acgcccatgg ttctgacgct ggaccagtta
      121 cgtccgaatc cggataaccc gcgcaccacg cgcaatccgc gttatgacga tatcaaaaat
      181 tccattcatg cccgcgggct ggatacggtg cccaaagtca cccgtatgcc ggacagtgag
      241 ccggatgtgt atatcttcag tgacggggga aacacccgtt accagatact gacgga
//
LOCUS       DAZKFU020000878          295 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.889, whole genome shotgun sequence.
ACCESSION   DAZKFU020000878 DAZKFU020000000
VERSION     DAZKFU020000878.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.889"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..204)
                     /gene="ycgZ"
                     /locus_tag="ACHOQQ_RS27480"
                     /old_locus_tag="pgaptmp_005496"
     CDS             complement(<1..204)
                     /gene="ycgZ"
                     /locus_tag="ACHOQQ_RS27480"
                     /old_locus_tag="pgaptmp_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HGU4473583.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKH"
BASE COUNT           86 a           61 c           56 g           92 t
ORIGIN      
        1 atgtttctgt tcctcttccc ctgtcgcttg ttccagtcgg caaagaagtt tggcacaaag
       61 cgactttcga ctcagattac gtccatcttt caaaatctca gtcacaattt ccccgagagt
      121 ttcctgctga gtaggcaagt tagcttttgc aaagtaacga gtgattgctc ccgcagaatc
      181 taaagtcact gaattttgat gcatgctacg cctctgttaa aaatgttaat caatgttaag
      241 tgaggtaaca atagcaaact tgtacgaaat atgtgtacag aatgataaat taatt
//
LOCUS       DAZKFU020000879          294 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.890, whole genome shotgun sequence.
ACCESSION   DAZKFU020000879 DAZKFU020000000
VERSION     DAZKFU020000879.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.890"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>294)
                     /locus_tag="ACHOQQ_RS27485"
                     /old_locus_tag="pgaptmp_005497"
     CDS             complement(<1..>294)
                     /locus_tag="ACHOQQ_RS27485"
                     /old_locus_tag="pgaptmp_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4473584.1"
                     /translation="FDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFDDPESLGF
                     AGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCYLWL"
BASE COUNT           75 a          110 c           66 g           43 t
ORIGIN      
        1 agccagagat aacagctgcc cccgtcatcc ggagaaacac gatccaccca gaacatctcg
       61 ccgttaatgg tcagcgtgtc accacgccgc acagcacgca ccgtatccgt ccgcacaaat
      121 aatgacgggc tgcttccttc aatacggacc ccgccaccgg caaaacccag cgactccgga
      181 tcgtcaaaaa ccccctgaac ttcgccgcca cgctgtgcac cggaggtgaa ctgcgcacgg
      241 atccccatca cttcaacgat cgtactgtcc accccggcga gggcagcatc aaag
//
LOCUS       DAZKFU020000880          292 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.891, whole genome shotgun sequence.
ACCESSION   DAZKFU020000880 DAZKFU020000000
VERSION     DAZKFU020000880.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.891"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..>292
                     /locus_tag="ACHOQQ_RS27490"
                     /old_locus_tag="pgaptmp_005498"
     CDS             48..>292
                     /locus_tag="ACHOQQ_RS27490"
                     /old_locus_tag="pgaptmp_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473585.1"
                     /translation="MMRFSLFTDTKKRVSSPVSQHDGEAVSPDMKERVAPQVRKPLSR
                     PGKMTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLL"
BASE COUNT           73 a           67 c           91 g           61 t
ORIGIN      
        1 tacagccgga cgcagtatgc catgccgggt gaacgaacgg aggcgttgtg atgcgatttt
       61 ccctgtttac agacacaaaa aaacgggttt cttccccggt gtcgcagcat gacggagaag
      121 cggtatctcc ggatatgaaa gaaagagtgg cccctcaggt acgaaagcca ctgtcaaggc
      181 cggggaagat gacccgacag gatgagacga aggtttacca tgcctgtccg tcggtgattg
      241 attttctgcc atgggtggaa tatctggacg aagagcagtg cctgctgctg ga
//
LOCUS       DAZKFU020000881          291 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.892, whole genome shotgun sequence.
ACCESSION   DAZKFU020000881 DAZKFU020000000
VERSION     DAZKFU020000881.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.892"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..138)
                     /locus_tag="ACHOQQ_RS27495"
                     /old_locus_tag="pgaptmp_005499"
     CDS             complement(<1..138)
                     /locus_tag="ACHOQQ_RS27495"
                     /old_locus_tag="pgaptmp_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4473586.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YE"
BASE COUNT           79 a           79 c           64 g           69 t
ORIGIN      
        1 ttcgtaggcc agagttacct gcaccactgt atttcccctg tgaatattca gctcacataa
       61 ccgttccctg aatatcagtg aaaaacacaa caccgtggcg cacacggcga gcaacccgat
      121 aagggcatat ttcgtcataa actttgacct ctctgcaagg aagaggctac aatcagtagt
      181 gttcaagtac ttcatgcagg cctcacgagt taatggatta acaagtgggg tcttcgcatt
      241 tctggtacct gcgtcttgcc tgaaccagaa aggctcagac acccgccgcg a
//
LOCUS       DAZKFU020000882          290 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.893, whole genome shotgun sequence.
ACCESSION   DAZKFU020000882 DAZKFU020000000
VERSION     DAZKFU020000882.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.893"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           88 a           61 c           54 g           87 t
ORIGIN      
        1 agctttgaga catctctatg agtctcaaaa cagttaaaaa ataaaaatta tttttacccg
       61 gtcactttgt aaaaaaaagt gaccgggttt tttgatttct gtgtttcgtg cgcttaatcg
      121 agtctcacgc ttcagtgagc aaattgcccc tccccaacag acatcacgat tcacaaggtc
      181 aaaaattctg ttccatgaag taccgtacaa agataaagag aaaaatgcct tatgcgggat
      241 gtcgctgtcg cgtctttatc gggaaactat acgtaattgc cactttgtct
//
LOCUS       DAZKFU020000883          290 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.894, whole genome shotgun sequence.
ACCESSION   DAZKFU020000883 DAZKFU020000000
VERSION     DAZKFU020000883.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.894"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>290
                     /locus_tag="ACHOQQ_RS27500"
                     /old_locus_tag="pgaptmp_005500"
     CDS             <1..>290
                     /locus_tag="ACHOQQ_RS27500"
                     /old_locus_tag="pgaptmp_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473587.1"
                     /translation="LGDTPLVDFTRDRLHRETLRRFGRYELTTAYTPVGQLQRQHLNS
                     LQYDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYAT"
BASE COUNT           66 a          107 c           72 g           45 t
ORIGIN      
        1 ctcggcgaca caccgctggt ggatttcacc cgcgaccgcc tgcaccggga aacgctgcgc
       61 agattcggcc gttatgaact caccaccgct tatacccctg tcgggcagtt acagcgtcag
      121 cacctgaaca gcctgcagta tgaccgcgat tacacctgga acgacaacgg cgaactcatc
      181 cgcatcagca gcccgcgcca gacccggagt tacagctaca gcaccaccgg caggctgacc
      241 ggcgttcaca ccaccgcagc gaatctggat atccgcatcc cgtatgccac
//
LOCUS       DAZKFU020000884          290 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.895, whole genome shotgun sequence.
ACCESSION   DAZKFU020000884 DAZKFU020000000
VERSION     DAZKFU020000884.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.895"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>290
                     /locus_tag="ACHOQQ_RS27505"
                     /old_locus_tag="pgaptmp_005501"
     CDS             <1..>290
                     /locus_tag="ACHOQQ_RS27505"
                     /old_locus_tag="pgaptmp_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473588.1"
                     /translation="LGDTPLVDFTRDRLHRETLRSFGHYELTTAYTPAGQLQSQHLNS
                     LQYDRDYTWNDNGELIRISSPRHTRSYSYSNSGRLTGVHTTTSTLDIRIPYAT"
BASE COUNT           67 a          110 c           67 g           46 t
ORIGIN      
        1 ctcggcgaca caccgctggt ggatttcacc cgcgaccgcc tgcaccggga aacgctgcgc
       61 agcttcggcc attatgaact caccaccgct tatacccctg ccgggcagtt acagagccag
      121 cacctgaaca gcctgcagta tgaccgcgat tacacctgga acgacaacgg cgaactcatc
      181 cgcatcagca gcccgcgcca tacccggagt tacagctaca gcaactccgg caggctgacc
      241 ggcgttcaca ccaccacctc aactctggat atccgcatcc cgtatgccac
//
LOCUS       DAZKFU020000885          289 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.896, whole genome shotgun sequence.
ACCESSION   DAZKFU020000885 DAZKFU020000000
VERSION     DAZKFU020000885.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.896"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>289)
                     /locus_tag="ACHOQQ_RS27510"
                     /old_locus_tag="pgaptmp_005502"
     CDS             complement(<1..>289)
                     /locus_tag="ACHOQQ_RS27510"
                     /old_locus_tag="pgaptmp_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473589.1"
                     /translation="IATYDESIATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFP
                     RDGADIFPDGWLENMTRRAERVFTSAKRQCGMLSASQAALFSQQTRQCASEQ"
BASE COUNT           74 a           78 c           73 g           64 t
ORIGIN      
        1 ttgctcactg gcacactgac gtgtttgttg agaaaaaagc gctgcctgac tggctgagag
       61 cattccacac tgacgtttcg cactcgtaaa aacacgttca gcccgacggg tcatattttc
      121 cagccacccg tcggggaaaa tgtcagcacc atcacgggga aacaaaatcg ctttccattc
      181 agggtatgag cgcggaatac ctccggaagc attccggctc agacagcgac tcacagtgca
      241 ggcagcacgg aatgcatatg ttgcgatgct ctcatcatat gttgcgatg
//
LOCUS       DAZKFU020000886          284 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.897, whole genome shotgun sequence.
ACCESSION   DAZKFU020000886 DAZKFU020000000
VERSION     DAZKFU020000886.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.897"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>284
                     /locus_tag="ACHOQQ_RS27515"
                     /old_locus_tag="pgaptmp_005503"
     CDS             <1..>284
                     /locus_tag="ACHOQQ_RS27515"
                     /old_locus_tag="pgaptmp_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4473590.1"
                     /translation="MPQLTATEAAAQENQRVMGILTCQEAKGREQLATMLAGQQGMSV
                     EQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPDMTV"
BASE COUNT           71 a           72 c          102 g           39 t
ORIGIN      
        1 atgccgcaat taactgcaac ggaagccgcc gcgcaggaga accagcgagt gatggggatc
       61 ctgacgtgtc aggaagcgaa aggacgtgaa cagcttgcca cgatgctggc aggacaacag
      121 ggcatgagcg ttgaacaggc ccgggcgatt ctggccgcgg cagcaccgca gcagccggtg
      181 gcatccgcgc agagtgaagc cgatcgcatt atggcgtgtg aagaagctaa aggtcgtgaa
      241 caactggcgg caacgctggc ggcgatgccg gatatgacgg tgga
//
LOCUS       DAZKFU020000887          281 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.898, whole genome shotgun sequence.
ACCESSION   DAZKFU020000887 DAZKFU020000000
VERSION     DAZKFU020000887.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.898"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>281
                     /locus_tag="ACHOQQ_RS27520"
                     /old_locus_tag="pgaptmp_005504"
     CDS             <1..>281
                     /locus_tag="ACHOQQ_RS27520"
                     /old_locus_tag="pgaptmp_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HGU4473591.1"
                     /translation="IARPEGLFRDTLVTGQPWRGQQRRTRMVIYRWIGKNNHDPMPPV
                     AMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDRDIL"
BASE COUNT           53 a           69 c          106 g           53 t
ORIGIN      
        1 attgcccgac cggaaggtct gttcagggat acgctggtca caggtcagcc atggcgtggt
       61 cagcagcggc gcacgaggat ggtgatttac cggtggatcg ggaaaaacaa ccacgatccg
      121 atgccgccgg tggcaatgct gaaccaggtc tgttcgcggg tcgtgggggc gctgggtggc
      181 gcaggcgtcc gctgtacacg gatgaacggg cagcaggtgc atggctggct gctgaggctg
      241 ttcaatcccc ggccggaatg ggtggacagg gatattctgt a
//
LOCUS       DAZKFU020000888          281 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.899, whole genome shotgun sequence.
ACCESSION   DAZKFU020000888 DAZKFU020000000
VERSION     DAZKFU020000888.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.899"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>281
                     /locus_tag="ACHOQQ_RS27525"
                     /old_locus_tag="pgaptmp_005505"
     CDS             <1..>281
                     /locus_tag="ACHOQQ_RS27525"
                     /old_locus_tag="pgaptmp_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4473592.1"
                     /translation="LRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQIINDELYL
                     DGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT           83 a           68 c           59 g           71 t
ORIGIN      
        1 ttaaggtcgg aactactcga ttcacgtttt ggtgcgaagt ctatttccac tatcgcagaa
       61 tcaaaacgtt ttccgctgca cgaaatgcgc gacgatgtcg catttcagat tatcaatgac
      121 gaattatatc ttgatggcaa cgctcgtcag aacctggcca ctttctgcca gacctgggac
      181 gacgaaaatg tccacaagtt gatggattta tccattaaca aaaactggat cgacaaagaa
      241 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa a
//
LOCUS       DAZKFU020000889          278 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.900, whole genome shotgun sequence.
ACCESSION   DAZKFU020000889 DAZKFU020000000
VERSION     DAZKFU020000889.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.900"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>278
                     /locus_tag="ACHOQQ_RS27530"
                     /old_locus_tag="pgaptmp_005506"
     CDS             <1..>278
                     /locus_tag="ACHOQQ_RS27530"
                     /old_locus_tag="pgaptmp_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HGU4473593.1"
                     /translation="PEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDN
                     LSLFIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGK"
BASE COUNT           67 a           69 c           68 g           74 t
ORIGIN      
        1 cctgaattca aaaaaatcat gatcaacgat gtactgcctc cgacacatga ccgtcacttt
       61 tttggcggtg atgcgacaag ctttgagcag attgaagcgg cagatatttt ttctattggc
      121 ctggtggaca atctctccct gttcattgac gaaatggcgc atccgttaca gccggttcgt
      181 ctgtccggtg atgaacttca cggagaagat ccatattacg tcctgtacgt cacgccgcgt
      241 cagtggaatg actggtacac ctcgacgtcc ggtaagga
//
LOCUS       DAZKFU020000890          277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.901, whole genome shotgun sequence.
ACCESSION   DAZKFU020000890 DAZKFU020000000
VERSION     DAZKFU020000890.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.901"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>277)
                     /locus_tag="ACHOQQ_RS27535"
                     /old_locus_tag="pgaptmp_005507"
     CDS             complement(<1..>277)
                     /locus_tag="ACHOQQ_RS27535"
                     /old_locus_tag="pgaptmp_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HGU4473594.1"
                     /translation="RIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRR
                     HPVSWLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHV"
BASE COUNT           64 a           86 c           79 g           48 t
ORIGIN      
        1 ccacatgcac atcagacgga ttcaggaaat tatcccggtg gagccgggca agatgccgtc
       61 tgaccacggc ggtataacgt gcggccggcg caccacctgc cgggtggtga taacacccgg
      121 ccacctgcag ccagctgacc ggatggcgac gccggcattc ctgcagaatg accgctgccg
      181 tattcagact ggtgtaagga tcaaatgccg cccatgtgga cgggaaacga tgtccattcc
      241 agcccagatt gacctgtgct atcccgacat caatacg
//
LOCUS       DAZKFU020000891          277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.902, whole genome shotgun sequence.
ACCESSION   DAZKFU020000891 DAZKFU020000000
VERSION     DAZKFU020000891.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.902"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>277
                     /locus_tag="ACHOQQ_RS27540"
                     /old_locus_tag="pgaptmp_005508"
     CDS             <1..>277
                     /locus_tag="ACHOQQ_RS27540"
                     /old_locus_tag="pgaptmp_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4473595.1"
                     /translation="EQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVSR
                     RIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPR"
BASE COUNT           72 a           84 c           77 g           44 t
ORIGIN      
        1 ccgaacagga agcgctgatc cacgccctgc aggaaaagct gagcaaccgg gagcgcgaaa
       61 tagaccatct gcaggcgcag ctggataaac tccgccggat gaacttcggc agtcgttccg
      121 aaaaagtctc ccgccgtatc gcacaaatgg aagccgatct gaaccggctt cagaaagaga
      181 gcgatacgct gactggtagg gtgtatgacc cggcagtaca gcgtccgttg cgtcagaccc
      241 gcacccgtaa gccgttccct gaatcactac cccgtga
//
LOCUS       DAZKFU020000892          275 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.903, whole genome shotgun sequence.
ACCESSION   DAZKFU020000892 DAZKFU020000000
VERSION     DAZKFU020000892.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.903"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>275
                     /locus_tag="ACHOQQ_RS27545"
                     /old_locus_tag="pgaptmp_005509"
     CDS             <1..>275
                     /locus_tag="ACHOQQ_RS27545"
                     /old_locus_tag="pgaptmp_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473596.1"
                     /translation="VLLSGLMQGTAAWRLRGVMFSPELTVAGAVPNTCPDTPAWKAII
                     DDCDAVSGRKLGFNWLKMLRLILLSLIVLWGAGTLLSLLVNRTQIYQ"
BASE COUNT           50 a           69 c           80 g           76 t
ORIGIN      
        1 tgttttgctc agtggactga tgcaggggac tgctgcctgg cgtttacgcg gtgtcatgtt
       61 cagtcctgaa ctgaccgttg ccggagcggt accaaacaca tgccccgaca cacctgcctg
      121 gaaagctatt attgatgact gtgatgccgt cagcggcagg aagctgggct ttaactggct
      181 gaagatgctg cgattgattc tgctttccct gattgtcctg tggggggccg gtacactgct
      241 gtctctgctc gttaaccgta ctcagattta tcagg
//
LOCUS       DAZKFU020000893          272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.904, whole genome shotgun sequence.
ACCESSION   DAZKFU020000893 DAZKFU020000000
VERSION     DAZKFU020000893.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.904"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>272)
                     /locus_tag="ACHOQQ_RS27550"
                     /old_locus_tag="pgaptmp_005510"
     CDS             complement(<1..>272)
                     /locus_tag="ACHOQQ_RS27550"
                     /old_locus_tag="pgaptmp_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGU4473597.1"
                     /translation="HREVMLIWPDFIAYNPNSGQNEAFPASAYACGLRAKIDHEQGWH
                     KSLSNVAVNNVLGISVPVFWSLQAEDSDAETLNDKEITTLIKRNGFR"
BASE COUNT           66 a           75 c           68 g           63 t
ORIGIN      
        1 cggaagccgt tacgtttaat gagtgtggtg atctccttgt cattgagggt ttccgcgtca
       61 ctgtcctctg cctggagcga ccagaacacc ggcacagaaa tacccaggac attattcact
      121 gccacattgg acagggattt atgccagccc tgctcgtggt caatcttcgc acgcaacccg
      181 cacgcatacg cagatgcagg aaaagcctca ttctggccac tgttagggtt gtacgcgata
      241 aaatcaggcc agatcagcat aacttcacgg tg
//
LOCUS       DAZKFU020000894          272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.905, whole genome shotgun sequence.
ACCESSION   DAZKFU020000894 DAZKFU020000000
VERSION     DAZKFU020000894.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.905"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>272)
                     /locus_tag="ACHOQQ_RS27555"
                     /old_locus_tag="pgaptmp_005511"
     CDS             complement(<1..>272)
                     /locus_tag="ACHOQQ_RS27555"
                     /old_locus_tag="pgaptmp_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HGU4473598.1"
                     /translation="SEVIQRLIQERISVRNMRLVMEALALWSPREKDIITLVEHVRGA
                     LGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDAS"
BASE COUNT           76 a           68 c           53 g           75 t
ORIGIN      
        1 ggatgcatcg agattaagaa aagtaccgcc agcggtaggc ctgacaccat ctctgattct
       61 gtcctctatt tcaggcgaaa tcacaattgc tttaatttct ccagaatatg aaaacttatg
      121 gcaaatgtag cgacctaatg cacctcgtac atgctcgacg agtgtgatta tatctttctc
      181 tcgtgggctc cataatgcta aggcctccat aactaaccgc atattgcgca ctgaaattcg
      241 ctcctggatc agacgctgaa ttacctctga aa
//
LOCUS       DAZKFU020000895          271 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.906, whole genome shotgun sequence.
ACCESSION   DAZKFU020000895 DAZKFU020000000
VERSION     DAZKFU020000895.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.906"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..>271
                     /locus_tag="ACHOQQ_RS27560"
                     /old_locus_tag="pgaptmp_005512"
     CDS             33..>271
                     /locus_tag="ACHOQQ_RS27560"
                     /old_locus_tag="pgaptmp_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4473599.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSH"
BASE COUNT           90 a           50 c           50 g           81 t
ORIGIN      
        1 gaccttccat accagttaat taaggatgat ttatgaaaaa gtatattatt cccttcgttg
       61 cgacaacgtt gatgtttagc gcaacaacct tcgccaagga tgtcgatcaa ggaacactaa
      121 ccattagagg agaagttgtt gatacaacgt gttactttgt taatggtgat tcgacaacag
      181 atatcgttgt cgaagatgtt gtaaccagtg caatgaatca acttatcaat aatgaaatct
      241 atactttaaa aaccggttca acctctcatg a
//
LOCUS       DAZKFU020000896          270 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.907, whole genome shotgun sequence.
ACCESSION   DAZKFU020000896 DAZKFU020000000
VERSION     DAZKFU020000896.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.907"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(26..253)
                     /gene="mchB"
                     /locus_tag="ACHOQQ_RS27565"
                     /old_locus_tag="pgaptmp_005513"
     CDS             complement(26..253)
                     /gene="mchB"
                     /locus_tag="ACHOQQ_RS27565"
                     /old_locus_tag="pgaptmp_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HGU4473600.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
BASE COUNT           83 a           78 c           51 g           58 t
ORIGIN      
        1 gcctgtaaaa taaataaaaa tataattagc taccgccacc agcagaagaa ctggcactac
       61 cacttcccac ggtcgagcca attgctgttg tcaaaccggc agatacggct ccaactacaa
      121 ccccaagtgg accaccaggt attccggcga gagctccaac aatagctgca gcacctgcag
      181 cattagctga agtagctggc gcacctcccg ccccggaaat atatcttaac tgtgattctg
      241 ttatttctcg catataaact gcatataaac
//
LOCUS       DAZKFU020000897          270 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.908, whole genome shotgun sequence.
ACCESSION   DAZKFU020000897 DAZKFU020000000
VERSION     DAZKFU020000897.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.908"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            50..>270
                     /locus_tag="ACHOQQ_RS27570"
                     /old_locus_tag="pgaptmp_005514"
     CDS             50..>270
                     /locus_tag="ACHOQQ_RS27570"
                     /old_locus_tag="pgaptmp_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4473601.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSV"
BASE COUNT           74 a           53 c           80 g           63 t
ORIGIN      
        1 tggcgatggc gatgggctcc ctgggatgca ggtttcacag gagaacgtca tgagcagttc
       61 gtttcagaat gagatcccga aggcaagggt gaatctgaaa ttagacctac atacaggtgg
      121 tgggcagaag aaagtggagc ttccgctcaa actgctggtt gccggtgatt tcagtaacgg
      181 acaggaagag cgcccgctgt cagagcgcat aaaaatggat gttaataaaa ataactttaa
      241 tgctgttctg gctgatatct cgccgtcagt
//
LOCUS       DAZKFU020000898          269 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.909, whole genome shotgun sequence.
ACCESSION   DAZKFU020000898 DAZKFU020000000
VERSION     DAZKFU020000898.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.909"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>269
                     /locus_tag="ACHOQQ_RS27575"
                     /old_locus_tag="pgaptmp_005515"
     CDS             <1..>269
                     /locus_tag="ACHOQQ_RS27575"
                     /old_locus_tag="pgaptmp_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HGU4473602.1"
                     /translation="VALADICARLNGRAPKSVCWMARRFGLKRLGYWSAEEDEILRQY
                     YPSEGVKVSRRLSGRTENAVKLRARELGVKFTGNGRLRLWTAEEW"
BASE COUNT           64 a           56 c           92 g           57 t
ORIGIN      
        1 gcgttgcgct tgctgatatt tgtgcccgtc tgaacggacg agcgccgaaa tccgtgtgct
       61 ggatggcccg caggtttggt ctgaaacgcc tgggttactg gagtgcagaa gaagatgaaa
      121 tcctgcggca atattatcct tcagaaggtg tgaaagtgag ccgacgcctt tccggacgta
      181 cagaaaatgc agtgaaactg agagcgcggg agctgggtgt aaagttcacg ggaaacgggc
      241 gattacgtct ctggacggca gaggagtgg
//
LOCUS       DAZKFU020000899          268 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.910, whole genome shotgun sequence.
ACCESSION   DAZKFU020000899 DAZKFU020000000
VERSION     DAZKFU020000899.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.910"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..146)
                     /locus_tag="ACHOQQ_RS27580"
                     /old_locus_tag="pgaptmp_005516"
                     /pseudo
     CDS             complement(1..146)
                     /locus_tag="ACHOQQ_RS27580"
                     /old_locus_tag="pgaptmp_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           63 a           70 c           61 g           74 t
ORIGIN      
        1 tggtacgtca gtttaagccg gatcccgtcg gtgtgactga cacctgtgat ccggtactgg
       61 ctgtcgcgct caaacgtgat gcggttgctg gaggtgtcac tcatcccgcc cagcatatac
      121 atccggtcgt cggggttgtg tgccaggtgt catatcaagt tggccttacg gaatttcaac
      181 tgcctccttg ccattaatca atatatcaat tgatgaagat aaattatccc catacttcca
      241 aagataatca tccaagccgc catatatc
//
LOCUS       DAZKFU020000900          266 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.911, whole genome shotgun sequence.
ACCESSION   DAZKFU020000900 DAZKFU020000000
VERSION     DAZKFU020000900.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.911"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>266
                     /locus_tag="ACHOQQ_RS27585"
                     /old_locus_tag="pgaptmp_005517"
     CDS             <1..>266
                     /locus_tag="ACHOQQ_RS27585"
                     /old_locus_tag="pgaptmp_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU4473603.1"
                     /translation="RAAQQKRIRSQQSPDGNRWPARKKRVFRVQAGVVFIWNGTQVRR
                     LKNWRTTTGRYGPMITGYDTERRGIRSFHKADIEEYLEIDTRRRT"
BASE COUNT           73 a           77 c           75 g           41 t
ORIGIN      
        1 cgcgccgcgc aacaaaagcg tatacgttcg caacaatcac cggacggtaa ccggtggccc
       61 gcccgtaaaa aacgtgtatt ccgtgtccag gcaggtgtgg tgttcatctg gaacggcact
      121 caggtgagac gactgaaaaa ctggcggaca acaaccggcc ggtacggccc catgatcacc
      181 ggttacgaca cggagcgccg gggaatacgc tcgttccaca aagccgatat tgaagaatac
      241 ctggaaattg acacccgcag gcgaac
//
LOCUS       DAZKFU020000901          265 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.912, whole genome shotgun sequence.
ACCESSION   DAZKFU020000901 DAZKFU020000000
VERSION     DAZKFU020000901.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.912"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>265
                     /locus_tag="ACHOQQ_RS27590"
                     /old_locus_tag="pgaptmp_005518"
     CDS             <1..>265
                     /locus_tag="ACHOQQ_RS27590"
                     /old_locus_tag="pgaptmp_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HGU4473604.1"
                     /translation="GSYMEGAEGNVFVLRKTYPNEIAAQRAAAARWQQLQRGAAEFSI
                     TLARGRAELYPEMHGTVTGFKTEIDNQDWLIAKVEHNIDGSGFT"
BASE COUNT           75 a           67 c           77 g           46 t
ORIGIN      
        1 aaggcagcta catggagggg gcagaaggta acgtgttcgt tctgagaaaa acctacccaa
       61 atgaaattgc tgcacagcgc gcagctgccg ccaggtggca gcaattacag cgcggtgcgg
      121 ctgaattctc catcacgctg gcccggggca gagcggagct ttaccctgaa atgcatggca
      181 cggtaaccgg ctttaaaacc gagatagaca atcaggactg gctcattgca aaagtggaac
      241 acaatatcga cggaagcggc ttcac
//
LOCUS       DAZKFU020000902          265 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.913, whole genome shotgun sequence.
ACCESSION   DAZKFU020000902 DAZKFU020000000
VERSION     DAZKFU020000902.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.913"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>265
                     /locus_tag="ACHOQQ_RS27595"
                     /old_locus_tag="pgaptmp_005519"
     CDS             <1..>265
                     /locus_tag="ACHOQQ_RS27595"
                     /old_locus_tag="pgaptmp_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HGU4473605.1"
                     /translation="DQRWCSDGFEFGCDDGAKLRVTFALDCCDREAIDWAASTGGYDK
                     ATVQDVMLGAIEKRFGDKGAERPIQWLTDNGSAYTAHETRQFAR"
BASE COUNT           68 a           57 c           84 g           56 t
ORIGIN      
        1 gaccagcgct ggtgttccga tggctttgag ttcggttgtg atgatggcgc aaaactgcgt
       61 gtcacgttcg cactggactg ttgcgatcgt gaagccatag actgggccgc aagcacggga
      121 ggttatgaca aagcaacggt gcaggatgtc atgttaggag caatagaaaa acgctttggc
      181 gataaaggag cggaacggcc aatccagtgg ctgactgata acggttcggc ttatacagct
      241 catgaaacac ggcagttcgc cagag
//
LOCUS       DAZKFU020000903          262 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.915, whole genome shotgun sequence.
ACCESSION   DAZKFU020000903 DAZKFU020000000
VERSION     DAZKFU020000903.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.915"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>262)
                     /locus_tag="ACHOQQ_RS27600"
                     /old_locus_tag="pgaptmp_005520"
     CDS             complement(<1..>262)
                     /locus_tag="ACHOQQ_RS27600"
                     /old_locus_tag="pgaptmp_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4473606.1"
                     /translation="SFFRRLSRECGFPVSPHRFRHTLATRLMKYPERNLHLVKSILGH
                     RSLTTTMGYVDVCMESAAQTLEDALSLYTDERTVSKICKGGN"
BASE COUNT           70 a           67 c           54 g           71 t
ORIGIN      
        1 ttattccccc ctttgcaaat cttgctcact gtgcgctcat ctgtatacaa actcagggca
       61 tcttccagtg tctgcgctgc agactccata cagacatcaa cataccccat ggtcgttgta
      121 agactcctgt gtcccagtat ggatttgacc agatgtaaat tacgttcagg atatttcatc
      181 agtctggttg ccagcgtatg tctgaaacga tgtggtgaaa caggaaaacc acattctcgc
      241 gaaagtcgcc ggaaaaatga ac
//
LOCUS       DAZKFU020000904          260 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.916, whole genome shotgun sequence.
ACCESSION   DAZKFU020000904 DAZKFU020000000
VERSION     DAZKFU020000904.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.916"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>260)
                     /locus_tag="ACHOQQ_RS27605"
                     /old_locus_tag="pgaptmp_005521"
     CDS             complement(<1..>260)
                     /locus_tag="ACHOQQ_RS27605"
                     /old_locus_tag="pgaptmp_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HGU4473607.1"
                     /translation="PYTDNRWNETYYRSTMIIGLAPEGKVRVWLGDRGNPVVPQLDAK
                     ITTVSGDKMNMCKGVTKHPDGYVYYGDTPDFIKGKIYPYGNW"
BASE COUNT           68 a           66 c           47 g           79 t
ORIGIN      
        1 caccagttgc cgtaggggta aattttccct ttgataaaat ctggtgtatc accgtaataa
       61 acatatccat ctggatgttt agtaaccccc ttacacatat tcattttatc acccgatact
      121 gtagtaattt tagcatccaa ctgaggtacc accggattac cgcgatctcc cagccagact
      181 cttaccttcc cttcaggtgc cagaccaata atcatcgttg aacgataata ggtttcgttc
      241 cagcggttgt ctgtgtaagg
//
LOCUS       DAZKFU020000905          259 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.917, whole genome shotgun sequence.
ACCESSION   DAZKFU020000905 DAZKFU020000000
VERSION     DAZKFU020000905.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.917"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>259)
                     /locus_tag="ACHOQQ_RS27610"
                     /old_locus_tag="pgaptmp_005522"
     CDS             complement(<1..>259)
                     /locus_tag="ACHOQQ_RS27610"
                     /old_locus_tag="pgaptmp_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HGU4473608.1"
                     /translation="YLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMI
                     ALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFV"
BASE COUNT          116 a           55 c           32 g           56 t
ORIGIN      
        1 ttaacaaagt aaatcaaaca actcagaaaa gccaaactaa gtatacagtt tgaaaattgc
       61 ctacggtcat aaatgaaata caactgcata aaaataaatg caacagtatc gataaaaaat
      121 aaagcaatca ttaccatcag ataggttgat cgtggaacaa cttcctcaac gcctggctga
      181 ttaaaccaac tattcaccaa aaccaaagca agcccaaaca ataagctcac taagacagta
      241 ctactaattc tcaaatagt
//
LOCUS       DAZKFU020000906          258 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.918, whole genome shotgun sequence.
ACCESSION   DAZKFU020000906 DAZKFU020000000
VERSION     DAZKFU020000906.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.918"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>258)
                     /locus_tag="ACHOQQ_RS27615"
                     /old_locus_tag="pgaptmp_005523"
     CDS             complement(<1..>258)
                     /locus_tag="ACHOQQ_RS27615"
                     /old_locus_tag="pgaptmp_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473609.1"
                     /translation="DALASKLATHAFFDRSNQGENKIEFVNEFVFGNYIANSIIANDS
                     DDWLANDERFVEPAVQAYLPRDSHDRELLWTRLSGMREFLSS"
BASE COUNT           81 a           56 c           38 g           83 t
ORIGIN      
        1 cgaactcaga aattcacgca ttccacttaa tcgtgtccat agtaattcac gatcatgact
       61 atctctcggt aaatatgctt gaacagctgg ttcaacaaag cgctcatcat tagctagcca
      121 atcatcagag tcattggcaa ttatactatt agcgatataa ttgccaaata cgaattcatt
      181 gacaaattct attttattct caccttgatt gcttctgtca aaaaaagcat gtgttgctaa
      241 tttacttgct agagcatc
//
LOCUS       DAZKFU020000907          256 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.919, whole genome shotgun sequence.
ACCESSION   DAZKFU020000907 DAZKFU020000000
VERSION     DAZKFU020000907.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.919"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>256)
                     /locus_tag="ACHOQQ_RS27620"
                     /old_locus_tag="pgaptmp_005524"
     CDS             complement(<1..>256)
                     /locus_tag="ACHOQQ_RS27620"
                     /old_locus_tag="pgaptmp_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473610.1"
                     /translation="FNTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAYLSRCLAS
                     IHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQR"
BASE COUNT           69 a           40 c           59 g           88 t
ORIGIN      
        1 ctcgttgttg accaaaaaag atatttatct tgatttcatt tgtactacgg caatcaatat
       61 gtagtaattt ttgatcgctg agattttctg tttcataaat gtttttggcg atagtcggat
      121 gtattgatgc cagacatctg ctcagataag ccagatcctt tagggaacac cccgctgagt
      181 agaggatgtc agagaatttt tgtgtgtcaa ctgttggctc tgaagcgtat gttgcattga
      241 caggaagagt attgaa
//
LOCUS       DAZKFU020000908          253 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.920, whole genome shotgun sequence.
ACCESSION   DAZKFU020000908 DAZKFU020000000
VERSION     DAZKFU020000908.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.920"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>253
                     /locus_tag="ACHOQQ_RS27625"
                     /old_locus_tag="pgaptmp_005525"
     CDS             <1..>253
                     /locus_tag="ACHOQQ_RS27625"
                     /old_locus_tag="pgaptmp_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473611.1"
                     /translation="AFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYG
                     WTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRP"
BASE COUNT           48 a           79 c           80 g           46 t
ORIGIN      
        1 cgcttttcct gataccctgc cgggttacac cgaatatggc cgggacaacg gcatccgtct
       61 gtctgccgtg tggctgacgc acgacccgga atacccggag aatttacctg ccgcgccgct
      121 ggtgcgctat ggctggacgc cacgcggcga actggcggcg gtgtatgacc gcagcaatac
      181 acaggtgcgc agctttactt acgatgataa ataccggggc cggatggtgg cgcaccgtca
      241 cacgggccga ccg
//
LOCUS       DAZKFU020000909          252 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.921, whole genome shotgun sequence.
ACCESSION   DAZKFU020000909 DAZKFU020000000
VERSION     DAZKFU020000909.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.921"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>252
                     /locus_tag="ACHOQQ_RS27630"
                     /old_locus_tag="pgaptmp_005526"
     CDS             <1..>252
                     /locus_tag="ACHOQQ_RS27630"
                     /old_locus_tag="pgaptmp_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473612.1"
                     /translation="DLLKSLNNIRQNATIYVHAYHYTDDEKCVMVPRISLPSLKLTPL
                     QSEIIIRQLLNGVNYIRHDGDVQGYEEKNVATFTIQEEG"
BASE COUNT           91 a           49 c           50 g           62 t
ORIGIN      
        1 tagacctgct gaaaagcctt aacaacatca ggcaaaatgc caccatatat gttcacgcat
       61 accactatac tgatgatgaa aaatgcgtta tggtgccacg gatatcttta ccatcactga
      121 aattaactcc gttgcaatca gaaataatta ttcgacaact tcttaatgga gtcaattata
      181 taaggcacga cggggatgtt cagggatatg aagaaaaaaa tgtggcaact ttcacaatac
      241 aggaggaggg ac
//
LOCUS       DAZKFU020000910          248 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.922, whole genome shotgun sequence.
ACCESSION   DAZKFU020000910 DAZKFU020000000
VERSION     DAZKFU020000910.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.922"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>248
                     /locus_tag="ACHOQQ_RS27635"
                     /old_locus_tag="pgaptmp_005527"
     CDS             <1..>248
                     /locus_tag="ACHOQQ_RS27635"
                     /old_locus_tag="pgaptmp_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HGU4473613.1"
                     /translation="RPEHHGDYYQPNNSGATTAHHYPVTSAGSLSIRMGAWGFCQQEY
                     TAWNPPRKFIRTVTGNFTGNGPWSEWKEVAILPLTQTG"
BASE COUNT           67 a           76 c           62 g           43 t
ORIGIN      
        1 agacctgaac atcatggcga ctactatcag cccaacaaca gcggagccac cactgcgcat
       61 cactacccgg taacatcagc cggatcatta tccatcagga tgggcgcatg gggattctgt
      121 cagcaggaat acaccgcctg gaatcctcca cgaaaattta tccggacagt gacaggtaac
      181 ttcaccggca acgggccgtg gtctgaatgg aaggaggttg ccatactccc cctcacgcag
      241 accggcga
//
LOCUS       DAZKFU020000911          248 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.923, whole genome shotgun sequence.
ACCESSION   DAZKFU020000911 DAZKFU020000000
VERSION     DAZKFU020000911.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.923"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(63..>248)
                     /locus_tag="ACHOQQ_RS27640"
                     /old_locus_tag="pgaptmp_005528"
     CDS             complement(63..>248)
                     /locus_tag="ACHOQQ_RS27640"
                     /old_locus_tag="pgaptmp_005528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473614.1"
                     /translation="SPVCVRGSMATSFHSDHGPLPVKLPVTVRINFRGGFQAVYSCWQ
                     NPHAPILMDNDPADVTG"
BASE COUNT           71 a           76 c           59 g           42 t
ORIGIN      
        1 agacctgaac atcatggtga ctactatcag cccaacaaca acggcgccac tacagcacat
       61 cactacccgg taacatcagc cggatcatta tccatcagga tgggcgcatg gggattctgc
      121 cagcaggaat acaccgcctg gaatccacca cgaaaattta tccggacagt gacaggtaac
      181 ttcaccggca acgggccatg gtctgaatgg aaggaggttg ccatactccc cctcacgcag
      241 accggcga
//
LOCUS       DAZKFU020000912          247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.924, whole genome shotgun sequence.
ACCESSION   DAZKFU020000912 DAZKFU020000000
VERSION     DAZKFU020000912.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.924"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>247)
                     /locus_tag="ACHOQQ_RS27645"
                     /old_locus_tag="pgaptmp_005529"
     CDS             complement(<1..>247)
                     /locus_tag="ACHOQQ_RS27645"
                     /old_locus_tag="pgaptmp_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004173387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473615.1"
                     /translation="ASLGQEWSGFGQSLFVQPLDLAWRQVLVPAAGSLNARWQTTIVD
                     QWNKAFAGRYPFKATGSDASLPLLAQFLRSDSGRITT"
BASE COUNT           56 a           82 c           66 g           43 t
ORIGIN      
        1 aacgtcgtga tccgacccga gtcgctacgc aggaactgtg ccagtaacgg cagggaggca
       61 tcactgccgg tggctttaaa cgggtaccgc cccgcaaaag ccttattcca ctgatcgact
      121 atggttgtct gccagcgggc attgaggctg cccgcagccg ggacaagcac ctgccgccag
      181 gccagatcca gcggttgcac aaacaacgac tgaccaaatc cactccattc ctgccccaga
      241 cttgctg
//
LOCUS       DAZKFU020000913          247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.925, whole genome shotgun sequence.
ACCESSION   DAZKFU020000913 DAZKFU020000000
VERSION     DAZKFU020000913.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.925"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>247)
                     /locus_tag="ACHOQQ_RS27650"
                     /old_locus_tag="pgaptmp_005530"
     CDS             complement(<1..>247)
                     /locus_tag="ACHOQQ_RS27650"
                     /old_locus_tag="pgaptmp_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HGU4473616.1"
                     /translation="ELLPPGEQSMLEIWQGKGSGGHSRHMTAARDTLRRPLTMPEQQA
                     DAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLAS"
BASE COUNT           43 a           81 c           65 g           58 t
ORIGIN      
        1 gaagccagat gcggaaaaac atacatcacc atgtcaggat tgggcagccg cggaaactgc
       61 tgactgatct cactttcctg tgtccggctg tagcttatgg ctccgtgcgc atctgcctgc
      121 tgctccggca ttgtcagcgg acgccgcagg gtgtccctgg ccgctgtcat gtgccgcgaa
      181 tgtcctccgc ttcctttccc ctgccagatc tccagcatac tctgttcacc cggaggcaga
      241 agctcct
//
LOCUS       DAZKFU020000914          246 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.926, whole genome shotgun sequence.
ACCESSION   DAZKFU020000914 DAZKFU020000000
VERSION     DAZKFU020000914.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.926"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           82 a           51 c           45 g           68 t
ORIGIN      
        1 tcattatcca ttccatccag acaaaacctg cctgtgtata aaaaataaga accatgtcac
       61 aaaaacattg cattccggca agttatgaca gaattactga aaaccgttat ctgtagttat
      121 tgtagtttct gatatatgct catccacccg gaaatacgac atggtcctga ctggcgttca
      181 gttcaaagtg taatttatta ttcacacact gagactgaaa aacggatggc gtggatgagt
      241 acacag
//
LOCUS       DAZKFU020000915          244 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.927, whole genome shotgun sequence.
ACCESSION   DAZKFU020000915 DAZKFU020000000
VERSION     DAZKFU020000915.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.927"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..89
                     /locus_tag="ACHOQQ_RS27655"
                     /old_locus_tag="pgaptmp_005531"
     CDS             <1..89
                     /locus_tag="ACHOQQ_RS27655"
                     /old_locus_tag="pgaptmp_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473617.1"
                     /translation="YMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(55..177)
                     /locus_tag="ACHOQQ_RS27660"
                     /old_locus_tag="pgaptmp_005532"
     CDS             complement(55..177)
                     /locus_tag="ACHOQQ_RS27660"
                     /old_locus_tag="pgaptmp_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473618.1"
                     /translation="MTTHPTGIYVGCFVKVIRRRLRLELRGRVINYSPFVLQHP"
BASE COUNT           72 a           57 c           47 g           68 t
ORIGIN      
        1 cttatatgaa cgccatggta gagttagcta agaacagagt accgctttat cacattatgg
       61 atgttgcaaa acaaatggag aataattaat aacacgccct cttagctcca gtcttagcct
      121 acgtcttatg accttaacaa agcacccgac ataaattcct gtcgggtgtg tcgtcatcat
      181 catcttacct tgcgaaaatc ctggcatacg tggtatgcgt cagggcatta gcaataccct
      241 tgat
//
LOCUS       DAZKFU020000916          244 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.928, whole genome shotgun sequence.
ACCESSION   DAZKFU020000916 DAZKFU020000000
VERSION     DAZKFU020000916.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.928"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           85 a           54 c           65 g           40 t
ORIGIN      
        1 gcaggctttt aaccggcaca taccggcaga agagcagtta cgtcgttatg atgaagcccg
       61 taaccagaat ggcagtgaac agcagcaaaa acaggctgaa atggcgctga atcagttgat
      121 taatcgttat cagatggaga acgcaggcaa accgaagagc cagccataat ggctgagata
      181 aaacatggaa atggaaaata cccccggaag agcgcgcaca ccatggacaa tcgaagatgt
      241 tcgc
//
LOCUS       DAZKFU020000917          243 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.929, whole genome shotgun sequence.
ACCESSION   DAZKFU020000917 DAZKFU020000000
VERSION     DAZKFU020000917.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.929"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>243)
                     /locus_tag="ACHOQQ_RS27665"
                     /old_locus_tag="pgaptmp_005533"
     CDS             complement(<1..>243)
                     /locus_tag="ACHOQQ_RS27665"
                     /old_locus_tag="pgaptmp_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4473619.1"
                     /translation="LSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGN
                     MDDEDWLRVSSAVGMALGDGGMGSLGERIIIDDCCEQ"
BASE COUNT           57 a           86 c           49 g           51 t
ORIGIN      
        1 ctgctcacag cagtcatcaa ttattatgcg ctcacccaga gagcccattc ctccgtcacc
       61 aagcgccata ccgaccgcag atgacaccct cagccagtcc tcatcatcca tgttgccact
      121 gcgcaaatga ttcagttcca cacgccccag catggaaagc atgcgcagaa tcagttgctc
      181 cttcggcatc tcaatgctga ataccagaac gctctcttcc gttcgccgca gtgcggcgct
      241 gag
//
LOCUS       DAZKFU020000918          242 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.930, whole genome shotgun sequence.
ACCESSION   DAZKFU020000918 DAZKFU020000000
VERSION     DAZKFU020000918.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.930"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>242
                     /locus_tag="ACHOQQ_RS27670"
                     /old_locus_tag="pgaptmp_005534"
     CDS             <1..>242
                     /locus_tag="ACHOQQ_RS27670"
                     /old_locus_tag="pgaptmp_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473620.1"
                     /translation="NCTINGTLKAENIIGDLVKCAGVAFPVDGSYLANGTRTLTVYDD
                     HSFDRQIIIPPIIYVGSKQESRTSNDIWTECFLHVD"
BASE COUNT           65 a           50 c           66 g           61 t
ORIGIN      
        1 aactgtacga taaatggcac gctgaaagcg gagaacatta ttggtgatct tgtgaaatgt
       61 gcaggggtgg cttttccggt ggatggtagt taccttgcga acggtacacg aacgctgacg
      121 gtgtatgacg atcacagctt tgaccggcag attataatcc cgccgataat ctatgtcggg
      181 tcaaaacagg aatcccgtac cagtaatgac atctggacag agtgcttcct gcatgttgat
      241 ca
//
LOCUS       DAZKFU020000919          242 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.931, whole genome shotgun sequence.
ACCESSION   DAZKFU020000919 DAZKFU020000000
VERSION     DAZKFU020000919.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.931"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>242
                     /locus_tag="ACHOQQ_RS27675"
                     /old_locus_tag="pgaptmp_005535"
     CDS             <1..>242
                     /locus_tag="ACHOQQ_RS27675"
                     /old_locus_tag="pgaptmp_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473621.1"
                     /translation="DSAMAWQLLGFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERV
                     LAARAEAENSFVVSVNGQEVDLSKLTSARLATLCEA"
BASE COUNT           58 a           47 c           85 g           52 t
ORIGIN      
        1 gacagtgcga tggcctggca actgctgggg tttccggatg tctgggttcg tcatgaagag
       61 cttgatgatg ttctgaaaaa gcaacaacag aatgagcagt tgcggcaggc acagcaggcg
      121 caggaaagag tgcttgctgc gcgggcagaa gcggagaaca gttttgttgt ttctgttaac
      181 gggcaggagg tggatttaag taagctcacc tcagcacggc tggcgacgct gtgtgaggca
      241 ga
//
LOCUS       DAZKFU020000920          242 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.932, whole genome shotgun sequence.
ACCESSION   DAZKFU020000920 DAZKFU020000000
VERSION     DAZKFU020000920.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.932"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>242
                     /locus_tag="ACHOQQ_RS27680"
                     /old_locus_tag="pgaptmp_005536"
     CDS             <1..>242
                     /locus_tag="ACHOQQ_RS27680"
                     /old_locus_tag="pgaptmp_005536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4473622.1"
                     /translation="LQAVDYGAAIINSSIDKSLLTLNLSPLKKDEIAVSVKVLDMNAI
                     FQGGHGTAGDLYKTTFYGPTQYYLLKKPKFGSVLMG"
BASE COUNT           76 a           40 c           61 g           65 t
ORIGIN      
        1 ggttacaggc tgttgattat ggggctgcaa ttattaacag cagcatagac aagtcattat
       61 tgactttaaa tctgtcacca ttgaaaaaag atgaaatagc tgtaagcgtg aaggtgttag
      121 atatgaacgc catctttcag gggggacacg ggacagccgg agatttgtat aaaacaactt
      181 tttacggccc gacacagtat tatctgctga aaaaacctaa atttggcagt gtgctgatgg
      241 gg
//
LOCUS       DAZKFU020000921          242 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.933, whole genome shotgun sequence.
ACCESSION   DAZKFU020000921 DAZKFU020000000
VERSION     DAZKFU020000921.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.933"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..188
                     /locus_tag="ACHOQQ_RS27685"
                     /old_locus_tag="pgaptmp_005537"
                     /pseudo
     CDS             1..188
                     /locus_tag="ACHOQQ_RS27685"
                     /old_locus_tag="pgaptmp_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           69 a           52 c           57 g           64 t
ORIGIN      
        1 tttgtctggt tcataaattc atcttctttg ttgagaagat taactgccta ggtgaaatta
       61 tcctctatga ccgggacgct gcaggccacc tggcagaagt catcacgcag gaagacaagt
      121 cgctgtatcc ctttgaaaaa tccggcaggc taatgggcat cttcagccca ggcggcacat
      181 cacagcgaat ttgaaggcaa atgcgaaaat tagtgatctt gaagttcata cagtattggt
      241 aa
//
LOCUS       DAZKFU020000922          241 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.934, whole genome shotgun sequence.
ACCESSION   DAZKFU020000922 DAZKFU020000000
VERSION     DAZKFU020000922.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.934"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          101 a           46 c           43 g           51 t
ORIGIN      
        1 aatgggcaat agttaatgct gcaccacttg cattcattaa tgttccaaaa acagcaccaa
       61 caagaaatat agggaagtat gcggatataa aactcacaac cgagtccata aatggaccgg
      121 tccatgaata aaaaacagga atatcacttc ccataaagac agccagcatt gccatcaaag
      181 gggatactat cagaactgga aaaccacgat atatgagata tattagtaaa ataagtgaaa
      241 t
//
LOCUS       DAZKFU020000923          240 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.denovo.935, whole genome shotgun sequence.
ACCESSION   DAZKFU020000923 DAZKFU020000000
VERSION     DAZKFU020000923.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.denovo.935"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..90)
                     /locus_tag="ACHOQQ_RS27690"
                     /old_locus_tag="pgaptmp_005538"
     CDS             complement(<1..90)
                     /locus_tag="ACHOQQ_RS27690"
                     /old_locus_tag="pgaptmp_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4473623.1"
                     /translation="MVSDTLPDGNHDRPWWGLPCTVTPCFGARL"
     gene            complement(163..>240)
                     /locus_tag="ACHOQQ_RS27695"
                     /old_locus_tag="pgaptmp_005539"
     CDS             complement(163..>240)
                     /locus_tag="ACHOQQ_RS27695"
                     /old_locus_tag="pgaptmp_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HGU4473624.1"
                     /translation="VLAVFAERRKDSFGPYVRLMSVTLN"
BASE COUNT           59 a           67 c           72 g           42 t
ORIGIN      
        1 cagacgggcg ccgaaacagg gcgtcacggt gcagggcagc ccccaccagg ggcggtcgtg
       61 attgccgtcg ggaagtgtgt ctgacacgat aaaatcctca cagaaaatcg gttaagaatg
      121 gtccggtggt ggcgataatt ctgctcatca gaatgccctg actcagttca gggtgacgct
      181 catcagccgg acatacgggc caaaactgtc cttacggcgt tcagcaaaca cggccagcac
//
LOCUS       DAZKFU020000924        17606 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.guided.084, whole genome shotgun sequence.
ACCESSION   DAZKFU020000924 DAZKFU020000000
VERSION     DAZKFU020000924.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.guided.084"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHOQQ_RS27700"
                     /old_locus_tag="pgaptmp_005540"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHOQQ_RS27700"
                     /old_locus_tag="pgaptmp_005540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU4473625.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHOQQ_RS27705"
                     /old_locus_tag="pgaptmp_005541"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHOQQ_RS27705"
                     /old_locus_tag="pgaptmp_005541"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU4473626.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHOQQ_RS27710"
                     /old_locus_tag="pgaptmp_005542"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHOQQ_RS27710"
                     /old_locus_tag="pgaptmp_005542"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU4473627.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHOQQ_RS27715"
                     /old_locus_tag="pgaptmp_005543"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHOQQ_RS27715"
                     /old_locus_tag="pgaptmp_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU4473628.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAGLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHOQQ_RS27720"
                     /old_locus_tag="pgaptmp_005544"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHOQQ_RS27720"
                     /old_locus_tag="pgaptmp_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU4473629.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHOQQ_RS27725"
                     /old_locus_tag="pgaptmp_005545"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHOQQ_RS27725"
                     /old_locus_tag="pgaptmp_005545"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU4473630.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="ACHOQQ_RS27730"
                     /old_locus_tag="pgaptmp_005546"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="ACHOQQ_RS27730"
                     /old_locus_tag="pgaptmp_005546"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU4473631.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="ACHOQQ_RS27735"
                     /old_locus_tag="pgaptmp_005547"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="ACHOQQ_RS27735"
                     /old_locus_tag="pgaptmp_005547"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4473632.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="ACHOQQ_RS27740"
                     /old_locus_tag="pgaptmp_005548"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="ACHOQQ_RS27740"
                     /old_locus_tag="pgaptmp_005548"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4473633.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="ACHOQQ_RS27745"
                     /old_locus_tag="pgaptmp_005549"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="ACHOQQ_RS27745"
                     /old_locus_tag="pgaptmp_005549"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4473634.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12901..14034
                     /locus_tag="ACHOQQ_RS27750"
                     /old_locus_tag="pgaptmp_005550"
     CDS             12901..14034
                     /locus_tag="ACHOQQ_RS27750"
                     /old_locus_tag="pgaptmp_005550"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4473635.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14123..14656
                     /gene="blc"
                     /locus_tag="ACHOQQ_RS27755"
                     /old_locus_tag="pgaptmp_005551"
     CDS             14123..14656
                     /gene="blc"
                     /locus_tag="ACHOQQ_RS27755"
                     /old_locus_tag="pgaptmp_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4473636.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="ACHOQQ_RS27760"
                     /old_locus_tag="pgaptmp_005552"
     CDS             complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="ACHOQQ_RS27760"
                     /old_locus_tag="pgaptmp_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4473637.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15146..15292)
                     /gene="ecnB"
                     /locus_tag="ACHOQQ_RS27765"
                     /old_locus_tag="pgaptmp_005553"
     CDS             complement(15146..15292)
                     /gene="ecnB"
                     /locus_tag="ACHOQQ_RS27765"
                     /old_locus_tag="pgaptmp_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4473638.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15403..15528)
                     /gene="ecnA"
                     /locus_tag="ACHOQQ_RS27770"
                     /old_locus_tag="pgaptmp_005554"
     CDS             complement(15403..15528)
                     /gene="ecnA"
                     /locus_tag="ACHOQQ_RS27770"
                     /old_locus_tag="pgaptmp_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4473639.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15580..16146)
                     /gene="efp"
                     /locus_tag="ACHOQQ_RS27775"
                     /old_locus_tag="pgaptmp_005555"
     CDS             complement(15580..16146)
                     /gene="efp"
                     /locus_tag="ACHOQQ_RS27775"
                     /old_locus_tag="pgaptmp_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4473640.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16188..17216
                     /gene="epmB"
                     /locus_tag="ACHOQQ_RS27780"
                     /old_locus_tag="pgaptmp_005556"
     CDS             16188..17216
                     /gene="epmB"
                     /locus_tag="ACHOQQ_RS27780"
                     /old_locus_tag="pgaptmp_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4473641.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVITSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4378 a         4612 c         4543 g         4073 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 ggcgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccgacgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg atcctggccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtgaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc actcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgaacagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggcttggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggtgcgac gattgtaggc agcattgaga ctgtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcgc
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aacttggctc taccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaacca gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccaga agtcatagag gcactccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagttattga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggga tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaacga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttgcgc tctgagctgg
     3661 ctgaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttgcgtaat caattgaaca
     3721 gccaacgcca gcttgaggca gagcgggcgc tggaaagtac cgaattacta gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa tctgctcggt gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacactac tgctggaact gaccaagctg aaagtctcta
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctattttacc cgttttctcg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccactga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgcga attttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatacc gctgtatctc aacaaagagg gcagcggcga caatattacc aaccatatgt
     5041 tgtggaacat gatgattggc gcgccactgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc acaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcatgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcacgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat tagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtgga aagtctggag ccgattaccc
     5521 tcggtgcggg gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaatc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtaccggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 caatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttactggt agcgtgacaa
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggctggcttc catgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac gctgacgtct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccggct tcaaccacag gttgaatgat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag tacgacaacc actgtcgcaa tcattgccga catacgcgtt
     6721 ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagga tgaagacagt aaccaacccg
     6901 cactgcatcc acatggcgat agacggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaacg ctttcggcgt cccctggatg atggctttca gcggtgaata gcaaagcgta
     7021 aagaacgcac cggtataagc gaggaacatc gaaagtccgg taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcgt tacccagcgt caccccgagg
     7141 ctcttcatca gcacataggt aatattgcca aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacgcc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatag caaataccac aaacgatagc
     7381 attgccagac cggactgata acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccccc caccgcagta atgcgggcaa ttttattaat cccctttgaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtgctcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagataccg
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaatcc aaatacggaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatctttt ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataaaac
     8041 gcgcccactg aactgcactg cccaacattg agcgggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttagcgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttatcacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcacccagcg
     8281 ccaacatcac cagacgatca acgcctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcttc aatcagattc tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttacc aatatttggt tctacgccaa aggtaaacag caattgtagc aacgtgtcgc
     8641 ggtcttcttc ggtatcagca acattgctca aatccagttt cgctgccact tcccgcagtt
     8701 gcgttttgtc ggcagagagc gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactcaag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaaac tgcggcacag ctggaatacc ggcccgcaac
     8941 cggcaaccag caggcgtttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaataa aacaacggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta
     9481 ccctgaagta cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccaaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtgggggt gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgtac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggtctgcca ggttccggta tcctgatgac
    10261 cgaaggttgc cgtggcgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagtct ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgccg cgtggcgatg tggtttatct cgacctgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 gctgcacggt gcaaaccgtc tgggctccaa ctccctggcg gaactggtgg tcttcggccg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggcgaaga tccgcgacga aatgggcctg gctatggaag aaggttgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggctg agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ctccagcgtg ttcaacaccg acctgctcta
    11041 caccattgaa ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcaccg agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctacc gccagctaaa cgcgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagctacc gctggtcctg ccgtatggcg atttgtggtt
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggcg aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctactgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttattg tgatcattaa cctgatcact
    12301 ctggcggcag ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtagttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg ctggttggta ttctgctgcc
    12601 gctggggctg tttccgggtg atgcactgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aatccacgta cctgcgggca aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtgtcgtta caatctaacg
    12841 catcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagaccct
    12901 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atcccgggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccgcaacaaa atactggcct gaacttaccg ctaaacagtg gaacgggatc
    13261 acgctattac atctcgcaac ctacaccgct ggcggcctgc cattgcaggt gccggatgag
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggcgcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg caaactcgtg tcttccagcc actcaaactc
    13501 aaccatacgt ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc
    13561 gaaggtaagg cagtgcatgt ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag
    13621 tcgaccattg aagatatggc ctgctgggta cgaagcaata tgaatccccg tgatatcaac
    13681 gacaaaacac ttcagcaagg gatacaactg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gcctgggctg ggaaatgctg gactggccgg taaatcctga cagcatcatt
    13801 aacggcagtg acaataaaat tgcactggca gcacgccccg taaaagcgat tacgcctcca
    13861 actcctgcag tacgcgcatc atgggtacat aaaacggggg cgaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctgca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctcc gcttttcctt gctgtcatct acacttagaa
    14101 aaaaaccagt aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc
    14161 atttctggtc gttgcctgca gttctcctac gccgccgcgt ggcgtgaccg tagtgaataa
    14221 tttcgacgcc aaacgttatc tcggtacctg gtatgagatt gcccgttttg atcaccgctt
    14281 tgagcgtgga ctggaaaaag tcaccgcaac atacagcctg cgtgatgacg gcggcctgaa
    14341 tgtcattaat aaaggctata accctgacag aggaatgtgg cagcagagtg aagggaaagc
    14401 gtactttacc ggcgcaccaa ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta
    14461 tggcggttat aacgttattg cactcgatcg ggaataccgc catgcgctgg tttgcggccc
    14521 ggaccgcgac tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca
    14581 ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc agtaaattta tttgggtaca
    14641 gcagcccggc agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc
    14701 cagactcgcc aggcgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatggc
    14761 cgcgccgact gcgccaatac ccgtccacac ggcataagcc gtccctactg gtaacgattt
    14821 catcgcccag gcaagtagcg ccatactgac aatcatcgcc gtcacggtaa taacactcgg
    14881 cgtcaaacga ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag
    14941 accagcaata actaagataa tccaggacat atcaggctcc ggagaattgg ggccgtcccc
    15001 ggtgaaaaga cgcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc
    15061 aggtggcagg ggaatttcaa ctattacctt tgtgactatc gcagagaaaa caaacggcac
    15121 gacacagctg tcgtaccgtt attgcttatt gctgcgcttt cgttgctgca ccagaaatcg
    15181 cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt ggtgttgcag gcagttaata
    15241 ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt cttcttcacc ataacgtctt
    15301 ccttttaggt aatgttattt atgtttgcct atagcaaatt aataatagac aacatcgcca
    15361 aaaatgatgg aaatctgatg atttttcgga agagaagaaa aattagctgg cagcgcggga
    15421 gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg cgagcggtgt tacagcccgt
    15481 gagcagcgtg ctggcaagca aaacaagaac gataaggcgt ttcatcatct ctgacctgaa
    15541 taagtgatgg tgcagcctgc aggccgcacc acaaccgcat tacttcacgc gagagacgta
    15601 ttcaccagag cgggtatcca ctttgatgac ttcgccgatt tgtacgaaca gcggaacttt
    15661 aaccacagcg ccagtagaca gggtagccgg tttgccgcca gtacctgcgg tatcaccttt
    15721 caggcccgga tcggtatcaa cgatttccag ttcaacgaag ttcggcggag taacggagat
    15781 cggctgacca ttccacagag ttacgataca ctctgcctga tccagcagcc atttcgcgtt
    15841 gtcgccaatt gcttttgcgt cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg
    15901 ccagaactca ccgtcgttgt acaggtaagt caggttcata tcgacaacat cagcgccttc
    15961 agcggaatca gtagatttga aggttttttc tacgcgagta ccggtcagca gacgacgcag
    16021 tttaacgcga gcaaatgcct ggcctttacc cggttttacg aattcactcg cttcaaccgc
    16081 gtaaggttcg ccgtctaaca tgattttaag accagcacga aaatcgttgc tatagtacgt
    16141 tgccataagg ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg gcgcatattg
    16201 taaccctaaa taccccatcc agagaagatt ggttaacgca acttgccgat gttgtgaccg
    16261 atcctgatga acttctgcgt cttttgaata tagacgcgga cgaaaaactg ttggccggac
    16321 gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc atttatcgat cgcatggaga
    16381 aaggcaatcc ggacgatccg cttttgcgtc aggtaattac ctcgcaagat gagtttgcca
    16441 tcgcgcccgg attctccacc gacccgctgg aagaacagca cagcgtagtg cctggtttgt
    16501 tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc
    16561 gttattgctt ccgtcgccac ttcccttatg cagaaaatca gggcaacaag cgtaactggc
    16621 aaactgcact tgagtatgtt gctgcgcatc cggaactgga cgagatgatt ttctccggcg
    16681 gcgatccgct gatggcgaaa gatcacgagc tggactggtt gctcacacaa ctggaagcca
    16741 tcccgcatat caaacgtctg cggattcaca gtcgtctgcc gattgtgatc ccggcacgca
    16801 tcacagatgc gctggttgaa cgcttttcac attctacgct gcaaatcttg ctggtgaatc
    16861 acatcaacca tgccaatgag atagatgaaa cattccgtca ggcgatggct aagttgcgcc
    16921 gggtaggcgt tactttgctg aaccagagcg ttctgttacg tgatgtgaac gataacgcac
    16981 aaacgctggc aaacctgagt aatgcgttgt tcgatgccgg cgtgatgccg tattacctgc
    17041 atgtgctcga taaagtacag ggtgcggcgc attttatggt gagtgatgac gaagcacggc
    17101 agattatgcg tgagttgctg acactggtgt cgggatatct ggtgccgaaa ctggcgcgag
    17161 aaattggcgg cgaacccagc aaaacgccgc tggatctcca gctacgccag cagtaactga
    17221 ttaaacaggg aagtcaaaca ttaaaattgc ctgatgcgct acgcttatca ggcctacatc
    17281 gtgcattgaa attgaatgga tttgcaatga actgtaggcc ggataaggcg tttacgccgc
    17341 atccggcaag agcaacaaac gcaagtacag caacctgtca ttaaacaggg aagtttaatc
    17401 tttgcccgtt gcataacctt taattattca tatgcattgc tgttgttact ctttatataa
    17461 ctaacaccaa taattaacct ttcttttctg tttatataga gtgagtacgg ctatttatgt
    17521 tattttaaca actattacta ccaagcccat ttatctggct tataaattaa aattaattat
    17581 ttaattgaga aatggttagg gagaac
//
LOCUS       DAZKFU020000925        10038 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.guided.177, whole genome shotgun sequence.
ACCESSION   DAZKFU020000925 DAZKFU020000000
VERSION     DAZKFU020000925.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.guided.177"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..368
                     /locus_tag="ACHOQQ_RS27785"
                     /old_locus_tag="pgaptmp_005557"
     CDS             <1..368
                     /locus_tag="ACHOQQ_RS27785"
                     /old_locus_tag="pgaptmp_005557"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4473642.1"
                     /translation="SQHYSPQQIAAWAQIDESRWKEKLAKSQVRVAVINAQPVGFISR
                     IEHYIDMLFVDPEYTRRGVASALLKPWIKSESELTVDASITAKPFFERYGFQTVKQQR
                     VECRGAWFTNFYMRYKPQH"
     gene            559..767
                     /locus_tag="ACHOQQ_RS27790"
                     /old_locus_tag="pgaptmp_005558"
                     /pseudo
     CDS             559..767
                     /locus_tag="ACHOQQ_RS27790"
                     /old_locus_tag="pgaptmp_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            787..1095
                     /locus_tag="ACHOQQ_RS27795"
                     /old_locus_tag="pgaptmp_005559"
                     /pseudo
     CDS             787..1095
                     /locus_tag="ACHOQQ_RS27795"
                     /old_locus_tag="pgaptmp_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(1152..2609)
                     /gene="pepD"
                     /locus_tag="ACHOQQ_RS27800"
                     /old_locus_tag="pgaptmp_005560"
     CDS             complement(1152..2609)
                     /gene="pepD"
                     /locus_tag="ACHOQQ_RS27800"
                     /old_locus_tag="pgaptmp_005560"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4473643.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2870..3328
                     /gene="gpt"
                     /locus_tag="ACHOQQ_RS27805"
                     /old_locus_tag="pgaptmp_005561"
     CDS             2870..3328
                     /gene="gpt"
                     /locus_tag="ACHOQQ_RS27805"
                     /old_locus_tag="pgaptmp_005561"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4473644.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3420..4664
                     /gene="frsA"
                     /locus_tag="ACHOQQ_RS27810"
                     /old_locus_tag="pgaptmp_005562"
     CDS             3420..4664
                     /gene="frsA"
                     /locus_tag="ACHOQQ_RS27810"
                     /old_locus_tag="pgaptmp_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4473645.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4722..5123
                     /gene="crl"
                     /locus_tag="ACHOQQ_RS27815"
                     /old_locus_tag="pgaptmp_005563"
     CDS             4722..5123
                     /gene="crl"
                     /locus_tag="ACHOQQ_RS27815"
                     /old_locus_tag="pgaptmp_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4473646.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5162..6217)
                     /gene="phoE"
                     /locus_tag="ACHOQQ_RS27820"
                     /old_locus_tag="pgaptmp_005564"
     CDS             complement(5162..6217)
                     /gene="phoE"
                     /locus_tag="ACHOQQ_RS27820"
                     /old_locus_tag="pgaptmp_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU4473647.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6505..7608
                     /gene="proB"
                     /locus_tag="ACHOQQ_RS27825"
                     /old_locus_tag="pgaptmp_005565"
     CDS             6505..7608
                     /gene="proB"
                     /locus_tag="ACHOQQ_RS27825"
                     /old_locus_tag="pgaptmp_005565"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGU4473648.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7620..8873
                     /gene="proA"
                     /locus_tag="ACHOQQ_RS27830"
                     /old_locus_tag="pgaptmp_005566"
     CDS             7620..8873
                     /gene="proA"
                     /locus_tag="ACHOQQ_RS27830"
                     /old_locus_tag="pgaptmp_005566"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGU4473649.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLDKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRV"
     gene            8988..9063
                     /locus_tag="ACHOQQ_RS27835"
                     /old_locus_tag="pgaptmp_005567"
     tRNA            8988..9063
                     /locus_tag="ACHOQQ_RS27835"
                     /old_locus_tag="pgaptmp_005567"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9021..9023,aa:Thr,seq:cgt)
     gene            complement(9190..9597)
                     /locus_tag="ACHOQQ_RS27840"
                     /old_locus_tag="pgaptmp_005568"
     CDS             complement(9190..9597)
                     /locus_tag="ACHOQQ_RS27840"
                     /old_locus_tag="pgaptmp_005568"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4473650.1"
                     /translation="MVVASPSYLAANSTPVHPRELVNHKCIGWRQSPDVAPYRWPFEE
                     NRRAFDLAIEPQITTNDLRLMLRLALAGGGITIATQETFRPYIESGRLVSLLDDFLPQ
                     FPGFYLYFPQRRNIAPKLRALIDHVKEWRQQLA"
     gene            9603..9869
                     /locus_tag="ACHOQQ_RS27845"
                     /old_locus_tag="pgaptmp_005569"
                     /pseudo
     CDS             9603..9869
                     /locus_tag="ACHOQQ_RS27845"
                     /old_locus_tag="pgaptmp_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT         2487 a         2477 c         2561 g         2513 t
ORIGIN      
        1 ccagtcagca ttattcacca caacaaattg ctgcctgggc gcagattgac gaatctcgct
       61 ggaaggagaa actcgcgaaa tcacaagtgc gggttgcagt cattaatgca caaccggttg
      121 gttttatttc ccgcattgaa cattatatcg atatgttatt tgttgaccct gaatacaccc
      181 gccgtggggt tgccagtgct ttgttaaaac cttggattaa gtctgaatcc gaacttacgg
      241 tggacgccag cataaccgca aaaccctttt ttgaacgtta tggttttcag acagttaagc
      301 agcagcgcgt tgaatgccgg ggagcgtggt ttactaattt ttatatgcga tataaaccgc
      361 aacattaaat ccagcttgca atgaaaataa cgcccgcctg gtatgtgcgc gttttctgat
      421 tccacaaact gcaaggaggt aaatcatgac aaatccttta tcaatgactc tttgcagacc
      481 tttccaggat taattctttt tttcttgccc tggattcgtc tgccatttcc tgatttttat
      541 tcataaatgg aatggtacat gggcaaatat attcgtccct tatccgatgc ggtatttacc
      601 atcgcatctg atgacctgtg gatcgagagt ttagcgatcc aacaattaca caccacggca
      661 aatttaccca acatgcagcg cgtagtgggg atgccagatt tacacacccc ggacgcggct
      721 acccgattgg cgcagcgttc ttctctgttg gtcgttttta cccggcaaga cgtcgcggta
      781 acggtgctgg aaaccgaagc cgggcgttat cctgacacgc tgcgttcgtc ggggccgggc
      841 ggtcaaaatg tcaataaaac cgacttggcg gtacgcgcca cgcatctggc atccggtatt
      901 agcgtgaagg ttcagtcaga gcgcagccag catgctaaca aacggctggc gcgattgctg
      961 attgcctgga ggctggagca gcagcaacag gaaaatagcg cggcgctgaa atcacagcgg
     1021 cgaatgttcc atcaccagat tgaacgtggc aacccgcgac gaacgtttac agggatggct
     1081 tttatcgaag gataatgaag gatgaaactg ccggaaggcg attaaacgcc atccggcagc
     1141 aaatcaaata attacttcgc cggaatttct ttcagcagtt cagtcagcag tgtccagtaa
     1201 tgacctacgc tttcgatgtg aacttgctca tccggagagt gtggaccggt gattgttggc
     1261 ccgatagaaa ccatgtccat ttccggatac ggttttttga acagaccaca ttccaggccc
     1321 gcgtggataa tctggatgtt cggcgtcttg ttgaacagac gctgataggt ttcacgtacc
     1381 agatgcatca ccggagagtt tgcgtccggc tgccagccag gatatgcacc tttcgcttcg
     1441 gttttcgcgc cagccagttt acccagcgaa tccagcatgc tcaccacgta gtctttaccg
     1501 ctgtcgatca gagaacggat cagacagtga atttctacat tattgtcagt catggtcacc
     1561 acaccgacgt tcagggaggt ttcgaccaca ccttttgcca cgtcggaatt gcggatcaca
     1621 ccgttcgggg tggcgttcag cagacgaata aaggtatcgc gagatttcgc aatcagggca
     1681 gctttatcgt tcgcaacaga gtccagcaac aaggccagat ttttctcttt ctctgccagc
     1741 tcgtttttca ggatctcctg ataggtattc accagagatt tcagggcgtc gactttatca
     1801 gccgcgacag caatggtcgc aaaggcttca cgcgggatgg cgttacgcag tgtgccgccg
     1861 ttgaaatcaa taagacgcag atccagttct tctgcatgac ccgccaggaa gcgcaccagc
     1921 agtttgttgg cattaccgag gccaacgtgg atttcaccgc cggagtgacc gcctttcaga
     1981 ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa cctcttcacg atctaaatgc
     2041 aggttggagg tgaagtcgat accccccgcg caacccatgt agatttcacc ttcttcttcg
     2101 gagtcggtgt taatcagaat atcagcctgc aaccagttgc tctgtaagcc gaacgcaccg
     2161 tccataccgg cttcttcggt catggtcagc agcacttcca gcgggccgtg aaccacgttt
     2221 tcgtcagcca gaaccgccag cgcagaggcc ataccaatgc cgttatccgc acccagcgtg
     2281 gtgccgcgcg ctttaaccca ttcgccatca atataaggct ggataggatc tttcgtgaag
     2341 tcatgcacgg tgtcgttatt tttctgcggc accatatcga ggtgggcctg caagacgacc
     2401 ggtttacgat tttccatacc tgctgtagca ggtttacgaa tcaagatatt acctacctga
     2461 tcgcgttcga catggaaacc tttctctttt gcccaaccaa caatgtattc agcgagttgc
     2521 tcttcatgat aggacgggtg aggaatagaa cagattttgg caaaaatatc ccacagcggc
     2581 tgtggagata attgagacag ttcagacacg ttaagtctcc ttgtcgatca cccgcaaaac
     2641 agtattgcag gtcacagggt tagcagaaaa tgttgtcaac acaagacagg cttgcgagat
     2701 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
     2761 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
     2821 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
     2881 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
     2941 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgg taccgggtgc
     3001 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     3061 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     3121 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
     3181 tccaaaagcg cactttgtca ccatctttgc aaaaccggct ggtcgtccgc tggttgatga
     3241 ttatgttgtt gatatcccgc aagatacctg gatcgaacag ccgtgggata tgggcgtcgt
     3301 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     3361 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
     3421 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     3481 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
     3541 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     3601 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
     3661 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
     3721 gggccaccca ggcgatggtg tggcaacaaa aagcctgtgc ggaagaagat ccgcaactca
     3781 gtggtcgtca ctggctgcat gcggctacgt tgtacaacat tgccgcctat cctcatctga
     3841 aaggagatga cctggccgag caagcgcagg ctttgtcaaa ccgcgcctat gaagaggccg
     3901 ctcagcgtct gccgggcacg atgcggcaga tggagtttac cgtacccggc ggtgcgccca
     3961 tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg
     4021 gtggtctgga tgcgatgcag acggactatt acagcctgta tgaacgttat tttgcgccgc
     4081 gcggcattgc gatgctgact attgatatgc cgtcggtggg cttttcatca aaatggaagc
     4141 tcacccagga ctccagcctg ttgcatcagc acgtcttaaa ggcgctgcct aacgtaccgt
     4201 gggtggatca cactcgcgtc gcggcctttg gtttccgttt cggcgctaac gttgccgtgc
     4261 gtctggcata tcttgaatcg ccgcgtctga aagcggttgc ctgtcttggt ccggtagttc
     4321 ataccctgtt gagtgatttt aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc
     4381 tggcgagtcg tttggggatg catgatgctt ccgatgaagc gttgcgcgtg gagctgaatc
     4441 gctattcatt aaaagtgcaa ggattgctgg gacgtcgctg cccaacgcca atgttatcag
     4501 gctactggaa gaacgatccg ttcagcccgg aagaggactc acgcttaatc acctcatcat
     4561 ctgctgacgg taaattatta gagatcccat ttaacccggt gtatcggaat tttgacaaag
     4621 gtcttcagga aatcaccgac tggatcgaaa aacgcttgtg ttaaaaattt gctaaatttt
     4681 gccaatttgg taaaacagtt gcatcacaac aggagatagc aatgacgtta ccgagtggac
     4741 acccgaagag cagattgatc aaaaaattta ccgcactagg cccgtatatt cgtgaaggta
     4801 agtgcgaaga taatcgattc tttttcgatt gtctggctgt atgcgtcaac gtgaaaccgg
     4861 caccggaagt gcgtgagttc tggggctggt ggatggagct tgaagcgcag gaatcccgtt
     4921 ttacctacag ttaccagttt ggtctgttcg ataaagcagg cgactggaag agcgttccgg
     4981 taaaagacac tgaagtggtt gaacgactgg agcacaccct gcgtgagttt cacgagaaac
     5041 tgcgtgaact gctgacgacg ctgaatctga agctggaacc ggcggatgat tttcgtgacg
     5101 agccggtgaa gttaacggcg tgagtgaaat gtgccggatg catcacatcc ggcaataatc
     5161 attaaaactg ataggtcatg ccaaccgcga caatatcatc attattaata ttcaatttgt
     5221 tatcgctatc cagttggttg attttataat caacaaacgc tgacatattt ttgttgaaat
     5281 aatatgtagc cccgacatcg atataattga ccagatcttc atcaccgata ccttcaatat
     5341 ctttcccttt cgataagaca taacccagcg atggacgcag accaaaatca aactggtatt
     5401 gagcgaccgc ttcaaagttc tgtgtcttat tggcaaagcc gccagttatt ggcgtcattt
     5461 tgcgtgtttc agaataaaaa gttgccagat aaatattatt ggcatcgtat ttcagacctg
     5521 ttgcccacgc ttctgcacgc ttgcctgtgc cacggctttg caggttctgc tcgttggtgc
     5581 gatctgagtt ggtataggcc ccactaatgg cgaaatcgct gccgccaaag tcatatgtca
     5641 atgacgtgcc gaagccatcg ccgttttgct ttttaacgtc gcggttttcg tttttccctt
     5701 gatattgcag ggttaagttc aggccatcga taacgccgaa gaagtcggtg ttccgatacg
     5761 tcgccagacc gctggcgcgt ttggtcataa agttgtcggt ctgcgccgag gagtcgccac
     5821 caaattccgg gaacatatcg gtccaggctt ccacgtcata caacgcgccc aggttacgac
     5881 cataatcgaa agaacccaaa tctttatatt tcaatccggc aaaagcgaga cgcgtttttt
     5941 gctgtgcagt atcactctct gctttattac cggcaaactc tgcttcccaa cgaccataac
     6001 cagtcagttg atcgttaatt tgtgtttcgc ctttaaaacc aaaacggata taactctggt
     6061 cgccatcttt actgtcgtta tcactcatat aatgcatggc tttaactttg ccatagacat
     6121 ccagtttatt accgtctttg ttatatattt ctgcggcctg tacggatgca gatgccacaa
     6181 tgcccatcac cactaatgcc agagtgctct ttttcatttt cattcctgat tttaattaac
     6241 gcgcgaatat tctgcgggag agccccgttg aaaacaggaa agtttttaac ctgagattgt
     6301 taaagatata ttacagatta atgatattct taaaatgtgg taatttagta aatctgtaat
     6361 aaaagcgtaa acaactgccg ctacgcttgc tgatcccgcg caacaaaaca ccatgctttg
     6421 ctcgcagatg gttggcaacc gacgacagtc ctgctaaaac gttcgtttga tatcattttt
     6481 cctaaaattg aatggcagag aatcatgagt gacagccaga cgctggtggt aaaactcggc
     6541 accagtgtgc taacaggcgg atcgcgccgt ctgaaccgtg cccatatcgt tgaacttgtt
     6601 cgccagtgcg cgcagttaca tgccgccggg catcggattg ttattgtgac gtcgggcgcg
     6661 atcgccgccg gacgtgagca cctgggttac ccggaactgc cagcgactat cgcctcgaaa
     6721 caactgctgg cggcggtagg gcagagtcga ctgattcaac tgtgggaaca gctgttttcg
     6781 atttatggca ttcacgtcgg gcaaatgctg ctgactcgtg ctgatatgga agaccgtgaa
     6841 cgcttcctga acgcccgcga caccctgcgt gcgttgctcg ataacaatat cgttccggta
     6901 atcaatgaga acgatgctgt cgctacggca gagattaaag tcggcgataa cgacaacctt
     6961 tctgcactgg cggcgattct ggcgggtgcc gataaactgt tgttactgac cgatcaaaaa
     7021 ggtttgtata ccgctgaccc gcgcagtaat ccgcaggcag aattgataaa agatgtttac
     7081 ggtattgatg acgcactgcg cgcgattgcc ggtgacagcg tttcaggcct cggaactggc
     7141 ggcatgagta ccaaattgca ggccgcggac gtggcttgcc gtgcgggtat cgacaccatt
     7201 attgccgcgg gcagcaagcc gggcgtcatt ggtgatgtga tggaaggcat ttccgtcggt
     7261 acgctgttcc atgcccaggc gactccgctt gaaaaccgta aacgctggat tttcggtgcg
     7321 ccgcctgcgg gtgaaatcac ggtagatgaa ggggcaaccg ccgccattct tgaacgcggc
     7381 agctccctgt tgccgaaagg cattaaaagc gtgactggca acttctcgcg tggtgaagtc
     7441 atccgcattt gtaacctcga aggtcgcgat atcgcccacg gcgtcagtcg ttacaacagc
     7501 gatgcattac gccgtattgc cggacaccac tcgcaagaaa ttgatgcaat actgggatat
     7561 gaatacggcc cggttgccgt tcaccgtgat gacatgatta cccgttaagg agcaggctga
     7621 tgctggaaca aatgggcatt gccgcgaagc aagcctcgta taaattagcg caactctcca
     7681 gccgcgaaaa aaatcgcgtg ctggataaaa tcgccgatga actggaagca caaagcgaaa
     7741 tcatcctcaa cgctaacgcc caggatgttg ctgacgcgcg tgccaatggc ctcagcgaag
     7801 cgatgcttga ccgtctggca ctgacgcccg cacggctgaa aggcattgcc gatgatgtgc
     7861 gccaggtgtg caacctcgcc gatccggtgg ggcaggtaat cgatggcggc gtactggaca
     7921 gcggcctgcg tcttgagcgt cgtcgcgtac cgctgggggt tattggcgtg atttatgaag
     7981 cgcgcccgaa cgtgacggtt gatgtcgctt cgctgtgcct gaaaacaggt aacgcggtga
     8041 tcctgcgcgg tggcaaagaa acctgtcgca ctaacgctgc aacggtggcg gtgattcagg
     8101 acgccctgaa atcctgtagc ttaccggctg gtgccgtgca ggcgattgat aatcctgacc
     8161 gtgcgctggt cagtgaaatg ctgcgtatgg ataaatacat cgacatgctg atcccgcgcg
     8221 gcggggctgg tttgcataaa ctgtgccgcg agcagtcgac gatcccggtg atcacaggtg
     8281 gtataggcgt atgccatatt tacgttgatg aaagtgcaga gatcgctgaa gcattaaaag
     8341 tgatcgtcaa cgcgaaaact cagcgtccga gcacatgtaa tacggtagaa acgttgctgg
     8401 tgaataaaaa catcgccgat agcttcctgc ccgcattaag caaacaaatg gcggaaagcg
     8461 gcgtgacatt acacgcagat gcagctgcgc tgacacagtt gcaggcaggc cccgcgaagg
     8521 tggtggcggt taaagccgaa gagtatgacg atgagtttct gtcattagat ttgaacgtca
     8581 aaatcgtcag cgatcttgac gatgccatcg cccatattcg tgaacacggc acacaacact
     8641 ccgatgcgat cctgacccgc gatatgcgca acgcccagcg ttttgttaac gaagtggatt
     8701 cgtccgctgt ttacgttaac gcctctacgc gttttaccga cggcggccag tttggactgg
     8761 gtgcggaagt ggcggtaagc acacaaaaac tccacgcgcg tggcccaatg gggctggaag
     8821 cactgaccac ttacaagtgg atcggcattg gtgattacac cattcgtgtg taaataaaac
     8881 cgggtgatgc aaaagtagcc atttgattca caaggccatt gacgcatcgc ccggttagtt
     8941 ttaaccttgt ccaccgtgat tcacgttcgt gaacatgtcc tttcagggcc gatatagctc
     9001 agttggtaga gcagcgcatt cgtaatgcga aggtcgtagg ttcgactcct attatcggca
     9061 ccatttaaat caagtagtta ccccatattc aaatacacca cgtttcctcc tgtgccgtat
     9121 ttatgccatt gtaaccctgg caattaatca aaatactgtt ctgacatcag gcaatgcagg
     9181 tgcagacatt taagccaatt gctgccgcca ttccttgacg tggtcaatca gggcgcggag
     9241 ctttggtgca atattgcgac gctgtgggaa atacagatag aagcccggaa attgtggaag
     9301 aaagtcatca agcagagata caagcctacc gctttcaata tatggcctga aagtttcctg
     9361 agtggcaatt gttattcctc cgccggcaag agccagcctc aacatcagac gcagatcatt
     9421 agtcgtaatc tgcggttcaa tcgcaaggtc gaaagctctc ctgttttctt caaatggcca
     9481 gcgataaggc gcaacatccg gggattgacg ccagccgata cacttatgat tcacaagttc
     9541 acgcggatga acaggtgtgc tgttggctgc gagataggag ggagaggcaa cgaccatttc
     9601 acgctgcctg atttgcagta cgggctattt ctgatggctt acggcaggaa agctcgaaaa
     9661 tccgtgtgac ctgcgtaaat cctggcgttg tcgaaagtga actggcttca accattaccc
     9721 atgtggaaac catgaaggca atggacgcct accgttctgt tgcacttcaa cctgcggata
     9781 ttgcccgtgc ggtaaggcat attattgaat ctcccgaaag cgttgataca accgaaatta
     9841 ccatcagacc cactgcatct gcaaactgaa gaataacttt tacattcatg ttttattaaa
     9901 taggtcagaa ggaagattta tatatccctg tgtttttcat agcaagatgc attatgtatt
     9961 gctcaccccg aaggagatga aggtttaatt acagaaggag tattttctct tggggtaaat
    10021 atgactcatg tttaatac
//
LOCUS       DAZKFU020000926         1277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.guided.547, whole genome shotgun sequence.
ACCESSION   DAZKFU020000926 DAZKFU020000000
VERSION     DAZKFU020000926.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.guided.547"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27850"
                     /old_locus_tag="pgaptmp_005570"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27850"
                     /old_locus_tag="pgaptmp_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4473651.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27855"
                     /old_locus_tag="pgaptmp_005571"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27855"
                     /old_locus_tag="pgaptmp_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4473652.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          365 a          252 c          302 g          358 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattat tgagcaaaat aatttatatg tggccggatt tgttaatacg gcaacaaata
      361 ctttctacag attttcagat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttctgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggaga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcacgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
LOCUS       DAZKFU020000927         1277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.guided.548, whole genome shotgun sequence.
ACCESSION   DAZKFU020000927 DAZKFU020000000
VERSION     DAZKFU020000927.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.guided.548"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27860"
                     /old_locus_tag="pgaptmp_005572"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27860"
                     /old_locus_tag="pgaptmp_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4473653.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27865"
                     /old_locus_tag="pgaptmp_005573"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27865"
                     /old_locus_tag="pgaptmp_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4473654.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          364 a          252 c          303 g          358 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattat tgagcaaaat aatttatatg tggccggatt tgttaatacg gcaacaaata
      361 ctttctacag attttcagat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttctgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggaga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcgcgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
LOCUS       DAZKFU020000928         1277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.guided.549, whole genome shotgun sequence.
ACCESSION   DAZKFU020000928 DAZKFU020000000
VERSION     DAZKFU020000928.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.guided.549"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27870"
                     /old_locus_tag="pgaptmp_005574"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27870"
                     /old_locus_tag="pgaptmp_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4473655.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27875"
                     /old_locus_tag="pgaptmp_005575"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27875"
                     /old_locus_tag="pgaptmp_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4473656.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          364 a          252 c          302 g          359 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattat tgagcaaaat aatttatatg tggccggatt tgttaatacg gcaacaaata
      361 ctttctacag attttcagat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttttgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggcga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcacgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
LOCUS       DAZKFU020000929         1277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.guided.550, whole genome shotgun sequence.
ACCESSION   DAZKFU020000929 DAZKFU020000000
VERSION     DAZKFU020000929.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.guided.550"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27880"
                     /old_locus_tag="pgaptmp_005576"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27880"
                     /old_locus_tag="pgaptmp_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4473657.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27885"
                     /old_locus_tag="pgaptmp_005577"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27885"
                     /old_locus_tag="pgaptmp_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4473658.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          363 a          252 c          303 g          359 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattat tgagcaaaat aatttatatg tggccggatt tgttaatacg gcaacaaata
      361 ctttctacag attttcagat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttttgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggcga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcgcgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
LOCUS       DAZKFU020000930         1277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.guided.551, whole genome shotgun sequence.
ACCESSION   DAZKFU020000930 DAZKFU020000000
VERSION     DAZKFU020000930.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.guided.551"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27890"
                     /old_locus_tag="pgaptmp_005578"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27890"
                     /old_locus_tag="pgaptmp_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4473659.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27895"
                     /old_locus_tag="pgaptmp_005579"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27895"
                     /old_locus_tag="pgaptmp_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4473660.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          364 a          253 c          302 g          358 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattgt tgagcaaaat aatttatatg tagccggatt cgttaatacg gcaacaaata
      361 ctttctacag attttctgat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttctgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggaga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcacgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
LOCUS       DAZKFU020000931         1277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.guided.552, whole genome shotgun sequence.
ACCESSION   DAZKFU020000931 DAZKFU020000000
VERSION     DAZKFU020000931.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.guided.552"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27900"
                     /old_locus_tag="pgaptmp_005580"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27900"
                     /old_locus_tag="pgaptmp_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4473661.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27905"
                     /old_locus_tag="pgaptmp_005581"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27905"
                     /old_locus_tag="pgaptmp_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4473662.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          363 a          253 c          303 g          358 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattgt tgagcaaaat aatttatatg tagccggatt cgttaatacg gcaacaaata
      361 ctttctacag attttctgat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttctgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggaga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcgcgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
LOCUS       DAZKFU020000932         1277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.guided.553, whole genome shotgun sequence.
ACCESSION   DAZKFU020000932 DAZKFU020000000
VERSION     DAZKFU020000932.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.guided.553"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27910"
                     /old_locus_tag="pgaptmp_005582"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27910"
                     /old_locus_tag="pgaptmp_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4473663.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27915"
                     /old_locus_tag="pgaptmp_005583"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27915"
                     /old_locus_tag="pgaptmp_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4473664.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          363 a          253 c          302 g          359 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattgt tgagcaaaat aatttatatg tagccggatt cgttaatacg gcaacaaata
      361 ctttctacag attttctgat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttttgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggcga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcacgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
LOCUS       DAZKFU020000933         1277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2211S48511
            SAMN43283554-rid25107233.guided.554, whole genome shotgun sequence.
ACCESSION   DAZKFU020000933 DAZKFU020000000
VERSION     DAZKFU020000933.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283554
            Sequence Read Archive: SRR30325528
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351790.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351790.2
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:32:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,585
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,348
            CDSs (with protein)               :: 5,348
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 66 of 151
            Pseudo Genes (incomplete)         :: 98 of 151
            Pseudo Genes (internal stop)      :: 31 of 151
            Pseudo Genes (multiple problems)  :: 35 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283554-rid25107233.guided.554"
                     /serotype="O 27:H30"
                     /isolate="2211S48511"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-10"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27920"
                     /old_locus_tag="pgaptmp_005584"
     CDS             33..992
                     /gene="stxA2b"
                     /locus_tag="ACHOQQ_RS27920"
                     /old_locus_tag="pgaptmp_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4473665.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27925"
                     /old_locus_tag="pgaptmp_005585"
     CDS             1005..1268
                     /gene="stxB2b"
                     /locus_tag="ACHOQQ_RS27925"
                     /old_locus_tag="pgaptmp_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4473666.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          362 a          253 c          303 g          359 t
ORIGIN      
        1 cgtattactt cagccaaaag gaatacctgt atatgaagtg tatattgtta aaatgggtac
       61 tgtgcctgtt actgggcttt tcttcggtat cctattcccg ggaatttacg atagactttt
      121 cgactcaaca aagttatgtc tcttcgttaa atagtatacg gacagaaata tcgactcctc
      181 ttgagcatat atctcagggg accacatcgg tgtctgttat taaccacacc ccaccgggca
      241 gttattttgc tgtggatata cgagggcttg atgtctatca ggcgcgtttt gaccatcttc
      301 gtctgattgt tgagcaaaat aatttatatg tagccggatt cgttaatacg gcaacaaata
      361 ctttctacag attttctgat tttgcacata tatcagtgcc cggtgtgaca actgtttcca
      421 tgacaacgga cagcagttat accactctgc aacgtgtcgc agcgctggaa cgttccggaa
      481 tgcaaatcag tcgtcactca ctggtttcat catatctggc gttaatggag tttagtggaa
      541 atgccatgac cagagatgca tccagagcag ttttgcgttt tgtcactgtc acagcagaag
      601 ccttacggtt caggcaaata cagagagaat ttcgtctggc actgtctgaa actgctcctg
      661 tttatacgat gacaccggaa gaagtggacc tcacactgaa ctgggggcga atcagcaatg
      721 tgcttccgga gtttcgggga gagggtggtg tcagagtggg gcgaatatcc tttaataata
      781 tatcagcgat actgggcaca gtggcggtta tactgaattg ccatcatcag ggggcgcgtt
      841 ccgttcgcgc cgtgaatgaa gagatacaac cagaatgtca gataactggc gacaggccag
      901 ttataaggat aaacaatact ttatgggaaa gtaataccgc agctgctttt ctgaatcgca
      961 gggctcactc tttaaataca tccggagaat aacaggagtt aaatatgaag aagatatttg
     1021 tagcggcttt atttgctttt gtttctgtta atgcaatggc agctgattgt gcaaaaggta
     1081 aaattgagtt ctctaagtat aatgagaatg atacattcac agtaaaagtg gccgggaaag
     1141 agtactggac taaccgctgg aatctgcaac cgctactgca aagcgcacag ttaacaggaa
     1201 tgacggtaac aatcaaatca aatacctgtg cgtcaggttc aggatttgct gaagtgcagt
     1261 ttaattaata tcagaag
//
